LOCUS       DAZKLD010000001        80608 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.001, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000001 DAZKLD010000000
VERSION     DAZKLD010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 80608)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 80608)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..80608
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.001"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..273
                     /locus_tag="ACG8KR_000001"
     CDS             <1..273
                     /locus_tag="ACG8KR_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0199413.1"
                     /translation="IYFNYLKEIKPILIKLNDEKNELLLNTKFQLINSDGSSSDMLFY
                     EFDGENYHCFNRKDNSISMYADIKREVKKILKVEEQYYKEIEKRNQ"
     gene            442..1767
                     /locus_tag="ACG8KR_000002"
     CDS             442..1767
                     /locus_tag="ACG8KR_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0199414.1"
                     /translation="MENIEETFLKVLSPYISDSILRFQEQEKNYKFGFDIKNYNDSLH
                     VVFQYNKTGKIGIKEVHIFQIFLAKVNIEASKSKLNYTTLLDTNALFFSKKLLPYLHS
                     YEWALRKLIYLLAPAYFSDNWAEESIPNEIISSIKRKQIQAIGKFDSKNLLQWIDLYD
                     FEEYLFGENYILVTQENEENIVRYKEISQDQLLKLFQDNSHTISDSYSLWSEVFSKYM
                     DVKLEEIQTDMSLIREGRNIVSHNKEIEMALYQKLIKKLKRYTNQLEKAFQKILAGDI
                     AGEDLSDMSDDFEGYIGNYFSNIDLAGVTKLSESLSAMKVLNTDSMKKSMESIGQTLS
                     VQQEIMQNFDTEGIRRATEGINQSLQSIKILNTDSMKRSIESIGQTLSALSAQRAIMQ
                     NFDTKGIRSATEGINQSLQSMKIVNTDSMKRSMESIGKISSTNQVLKSE"
     gene            1867..2685
                     /locus_tag="ACG8KR_000003"
     CDS             1867..2685
                     /locus_tag="ACG8KR_000003"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0199415.1"
                     /translation="MVQGLFGGGTSYEGQSISDIKEDVNNWKIYTENVKSQIVEKKEA
                     LIKNGFWNKIPWNFQQTINESIFCLGTFLSDFQIVESAFEENFISEREIKLLRKIGKN
                     SVQFNHSYGKYFKEEYEWREYGNPEFEIVESIYQHGRDFFVTLQDASNAAGRLEDYMS
                     NNSIYNKVNIFGNSYNTNIQQGHNNNMIVSIDDMSELLSKLDLLLENLNDSLPEEEVE
                     EAQEYIETIKDEAIKEKPKKTMLRFAVDSLKKLKWSTEFLASVATIAQLVAPLL"
     gene            complement(3124..4269)
                     /locus_tag="ACG8KR_000004"
     CDS             complement(3124..4269)
                     /locus_tag="ACG8KR_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFQ0199416.1"
                     /translation="MATFKQYETKKGKFWLYEAYLGMNKMTGKPDKVRRRGYKTKKEA
                     QLALSRLQVDYDKNGIKKANNETFKEVYDLWLETYKTTVREATFMKTEIKFRKWILPK
                     YGNLRVNEVTVKKAQQIVNSWAETTDQYKVLHSTSKRIFSYAINLGIIETNPLDNVLM
                     PKRTTTKHKEKVKVYSKEQLITLFDYLNSKPETYRNDFDKTLLRFLFYSGVRISEALA
                     LNWSDIDFQENTVTINKTLSQSKNGYKISDPKTDCSAGVLPLDERTISILKKWQINQR
                     KYMLTLGVTDPTMIFCGIYKELITHHAIYARLITICEKAMVPFLGVHVARHTHASMLL
                     DAGATMVEVQTRLRHTKISQTIDCYGHLAKETKEKTVEKLVQHLNID"
     gene            complement(4330..4977)
                     /locus_tag="ACG8KR_000005"
     CDS             complement(4330..4977)
                     /locus_tag="ACG8KR_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFQ0199417.1"
                     /translation="MNGGENVARKKYPEAVETGQRIRLARIEKGLSMEQLGGKLSPPA
                     SKGAVSNWENGYNLPNNTRLKQLSKVLDVSTTYLLNGSYTLRDMHMMPEIKRNVLEKQ
                     LKKTIEKNNSIIDSNLSNAINNIEEDNLTVSQKIYLTNAIKYLKTGSRIEFPDGSTPI
                     AYLAALLNTLLRYDSLINDSEKNTDEKKEYQEFYENDLLKEFTALVTELNKHYSK"
     gene            5171..5464
                     /locus_tag="ACG8KR_000006"
     CDS             5171..5464
                     /locus_tag="ACG8KR_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0199418.1"
                     /translation="MELKIDKLTLDDPAFKIIDDRVVQQVEQTQGTLLSLLEKKYQFP
                     KYMNKAQAAKYMNVSYNTMMQKYVPNGLKLIIVDGVVRISQDECDRFMEEYQK"
     gene            5508..5819
                     /locus_tag="ACG8KR_000007"
     CDS             5508..5819
                     /locus_tag="ACG8KR_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0199419.1"
                     /translation="MESTQNKAIEKVLSTILTEDTAAELANLEGKALEETFECLYEQM
                     DYQKLLPQEPTVSGVLRGLNDLVQAEFKERLSIEEYQEILYQQVELLASLLGLELEDV
                     E"
     gene            5816..6440
                     /locus_tag="ACG8KR_000008"
                     /pseudo
     CDS             5816..6440
                     /locus_tag="ACG8KR_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350005.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage repressor protein"
     gene            6581..6739
                     /locus_tag="ACG8KR_000009"
     CDS             6581..6739
                     /locus_tag="ACG8KR_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0199420.1"
                     /translation="MKRNLIIGSILIGGIFGLLPLIAKIAIFAGLALYLFTAYDEYDY
                     QLRTGGHK"
     gene            6740..7102
                     /locus_tag="ACG8KR_000010"
     CDS             6740..7102
                     /locus_tag="ACG8KR_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0199421.1"
                     /translation="MYKTKLLNQLDSLELEEINQGIAELENNIGKTYFGNSFNEKLTV
                     LYVLKKHAEHKIICREINELKNQILTAWLNITDMQEARVKTFNTWVKYQNQLKGAEFV
                     RDGLKYELEQLKLMEVSE"
     gene            7139..7987
                     /locus_tag="ACG8KR_000011"
     CDS             7139..7987
                     /locus_tag="ACG8KR_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA primase/polymerase"
                     /protein_id="HFQ0199422.1"
                     /translation="MANNYQAATELQKSGWSIYPLSPGTKIPIKGSSGWNDATNDSNQ
                     INEWWTEDATRNIGLMLEDHQMVVVDLDQHSETRNGVENYKKLASMYDPFPSTYTEIT
                     PGKGIHFFFKIPDGVIIQQETGAFADIFGRDDKGKSLSGIDIITKGVPIAPTMIISRS
                     VGRAYEPIENVSLNNIAPAPDWLVNLLLKKPQEIKTQYRPVAKTGTAKKLDMIVQGAG
                     EGSRNDHLTKLCGWLLWHGVDNQTLAELIYTANAYNEPPLKDKEVNQIIRSMIKKDLR
                     GKAHGS"
     gene            7977..9452
                     /locus_tag="ACG8KR_000012"
     CDS             7977..9452
                     /locus_tag="ACG8KR_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VapE domain-containing protein"
                     /protein_id="HFQ0199423.1"
                     /translation="MAHKHKLDSILDFPEASEREDNIIELKTWMSRLRCNKDDQIKSN
                     SVVNAELILTNDSNLAGTIAYNEFSGYIHLLKDSPWINRTAGEWEDSFEDALTAYIEE
                     NYNVVFDDNKIHKAVVNVARKNVFNPVKERIEKVKWDQQPRLETLFIDLLGVDDNLYT
                     REVTKRWIVGSVARIYKPGIKFEIVPVLDGPQGIGKSTVPALLYTDDFFTDSLDSLGE
                     KKDDYMQLQGNVIIELGELSSMNKTKIEQIKNFISAKIDKIRPPYGRNVIAWARQCVF
                     IGTSNDGQYLKDDTGNRRFYPLPCKNKPKMNPFKTKDEYFLQVLAEAKVLFDKGQRIY
                     FSPDEDKEVLEIAKDYQEDAKMENPIKEAITKYLEMEIPYKWEEAPAWARRSYYQNYP
                     DSKCNEKNLQHFYNGATMQQEFYLVDSVLTADILEAVFDKQAKDLLNGRSDAETKKIA
                     LIISSIPGWERKQLPKRNKRRGFYNKSNAYENKNRAGKYKK"
     gene            9718..10125
                     /locus_tag="ACG8KR_000013"
     CDS             9718..10125
                     /locus_tag="ACG8KR_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3206 domain-containing protein"
                     /protein_id="HFQ0199424.1"
                     /translation="MLNIISTNKAPNFQYTDEMDRFLMNTLAFSVGLVTEDYSTFDPE
                     VLKIMEEEPDWLQESVAWCQSLVVGSLVDSGNYDDTGELMDEFNCLLNLYDRARQREL
                     TSNEDNLFLNIHDKFLALLLTDDELITNLLEVE"
     gene            10128..10343
                     /locus_tag="ACG8KR_000014"
     CDS             10128..10343
                     /locus_tag="ACG8KR_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0199425.1"
                     /translation="MHMESIRYFDGEKHIEYSNTQEDVDFISFEEDKITKVNFKDGTC
                     LKIVSPYIEYKSKWEQDDSDERDRFGW"
     gene            10347..10727
                     /locus_tag="ACG8KR_000015"
     CDS             10347..10727
                     /locus_tag="ACG8KR_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFQ0199426.1"
                     /translation="MAVKKQCNHAGCKTLIDYRQKYCGKHKAKQTAIKREERKQSEGK
                     YFQFYQSRTWRKASYLYRLNHPVCEDCLEEGLIRKADVVDHKIELKDDWNRRLDETNF
                     RSLCHAHHNSKTANERQRREKSTL"
     gene            10861..11016
                     /locus_tag="ACG8KR_000016"
     CDS             10861..11016
                     /locus_tag="ACG8KR_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2292 domain-containing protein"
                     /protein_id="HFQ0199427.1"
                     /translation="MKVGDLLKNTIVITDKTGKQRTIELPHFGQVTIQMQNGKIIYID
                     KLDKEKF"
     gene            11085..11558
                     /locus_tag="ACG8KR_000017"
     CDS             11085..11558
                     /locus_tag="ACG8KR_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HFQ0199428.1"
                     /translation="MGHPKILHDTKGNISSEEKAIRVDAREELFKQQPLINITPPDWM
                     AASARSEWNRIVPTLKKDYPLSEADYGSLVAYCLAFARMKTAEAEIRKSGTFITCENG
                     VKKANPAVRVQSQAMSDLKKQATSLGMTLESRSKLALNKVKNDEPEDPFKELMNS"
     gene            11555..13249
                     /locus_tag="ACG8KR_000018"
     CDS             11555..13249
                     /locus_tag="ACG8KR_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase TerL endonuclease subunit"
                     /protein_id="HFQ0199429.1"
                     /translation="MNDYIEKVLSGKLIAPKKIIQACERHISDLERSKSDSFPYVFDE
                     EQATKAIKFIELLPSTDGKAIKMLGFQKFILGSLYGWRTKEGNYRRFNRAFTSMSRKN
                     GKTYIASGMAANALIMEQEPAEARQVLFVSNALKQAKLGYDMLSNSLRNVVKSSKFLR
                     PQLKIMNSKIQHLPSNSFAMALASETSTLDGFAPTTAILDEWHEAKTRKTYNVIKSGM
                     TQQKNGLLCVISTAGLDLNVPMYEEYLLLDRVLKGEEQADRYFIAIWELDDPEEIHDQ
                     EKWIKANPIFESEEIKKVMIPTIQDDVNLALKQNNLNSVLVKNFNLWRQASEDSYMIA
                     EDWQATEVEPQDITGKPVYIGVDLSKTDDLTSVSWIVPLDNGELYCDSHSFVATKYGL
                     QDKEKRDGLPYRELEKAGECSITQLESGIVDYDQVFQFIQDLIQENDLECMGICYDPY
                     NANSLISKAEKANYPMLEVRQGTITLNVPTRTFREQVYEGNVIHNKNTILTHAVNNAI
                     LKTDNNGIQINKSKNSNKIDPIAALINAYVFAMDYFTTTEGAKADNEFYTSEEFSF"
     gene            13215..13400
                     /locus_tag="ACG8KR_000019"
     CDS             13215..13400
                     /locus_tag="ACG8KR_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0199430.1"
                     /translation="MNFIQVKNFLSNYIHTVLLLLGLVCVLVAITLLTNVYYGLLALG
                     IVLIGVAVMLNTEQKGG"
     gene            13404..14579
                     /locus_tag="ACG8KR_000020"
     CDS             13404..14579
                     /locus_tag="ACG8KR_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFQ0199431.1"
                     /translation="MAFFKARQNTTGDPFLQHVVSIQSDDYTTSFTSVRALRNSDVFA
                     AVRIIASDIASSPIQLVKNNMPQADNELVKLLNEKPNSEMDGWHLKFALAVNMLLNGN
                     SFAEIKRNGEKVEEIHLLPNSSVTVTQLDNGTLSYQIGDKKRRVKSSDILHFKYFTQD
                     GLTGLPPLYALRDELKIQQAGNRTLHNFFTRGVSGSGILKVHKSDLDGSAKNAIREKF
                     EEANGSSSGDNALRTIILDETMDYKTLEINTDVLKLINSNDWNTKQIAKAFGVPIERL
                     GVENEHSSTVQSNLQYIQSTLIHYFNVFVSELDTKLETNIRFNSDQLLETDPETKVKN
                     VLDQVKGSLLTINEGRSKMGLPPMDGGDRLLASLNFTYLDTLEKYQLKEQEGVTPVE"
     gene            14572..16095
                     /locus_tag="ACG8KR_000021"
     CDS             14572..16095
                     /locus_tag="ACG8KR_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFQ0199432.1"
                     /translation="MNNEEEKEKRLTEEAELTADSPKVEKENEKQPTDGKTISGYALK
                     FGQPSKDLGGFVEVITPEALKEVDLSNVFLLYGHDYSKPLASVKAGTLKLNVDDTGLH
                     FEAELTDTTYSNDVYENISKGVVDSMSFGFVLGLDSFDKKEDGTIERSIDKIKALNEI
                     SVVTVPAYDSSNVQVNKRSYESFMSNNQAKQTNNSLESTSKAQKESNNMEKTLIDNEK
                     TELRGYEEYIRSQGEVRDGVTTVNAAAVVPEEVIGEVFDLKRSNYNLAQYATVKTVSN
                     GQGKYPVATNQQAVLATKAELAEIGDIDAEMFTSVDYKVETRAGKIALSNEVVEDSAV
                     NIVQEVKDQLAKLVENTDNKHIMDLLKTFTKKTAATLDDLKQLYNVALDPALNKMVIL
                     NQSGYYHLDTLKDSDGRYILQPDVTAPSGKSLFGMPVVLIADTLFANPKAGTFPMIMG
                     DIAQSIFVARRNQVTTQWEKFDYYSQGLAVIVRNDYKKIDQNASVYIEFTPVVTPKV"
     gene            16151..16435
                     /locus_tag="ACG8KR_000022"
     CDS             16151..16435
                     /locus_tag="ACG8KR_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HFQ0199433.1"
                     /translation="MVILDSIKKSMRIDHTIDDDFIQQLIDTAGEYIKSAIDSSATDK
                     DMDNYQQFDLAVSLLTQHWYLNRQEASSERIPVTVQALVQQMRGAYYANH"
     gene            16422..16757
                     /locus_tag="ACG8KR_000023"
     CDS             16422..16757
                     /locus_tag="ACG8KR_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFQ0199434.1"
                     /translation="MPIIKNVNELTERVKFKKTKRVKDEDGQMVDSEETVFECWANVR
                     SQMLKDVLASVGTILEGTLTFIIRYDQDYELTNDMKVAWKNKSYKIISINEGTAFKDY
                     TTIIAKQIS"
     gene            17052..17678
                     /locus_tag="ACG8KR_000024"
     CDS             17052..17678
                     /locus_tag="ACG8KR_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0199435.1"
                     /translation="MDKAEFTNFYWKYYLHLENEFINTTDFVMLDENNFKTFSIEYQK
                     LLLAIGSECEIIFKELCGFDSSSNKSITDFKAVIKSSELMCLDNGVRVMNSLTLTSLK
                     PFGDGWPEDTPDWWKIYNRVKHGRSSNYKEANLENVLYALASLYLLEEYLHKKVILEG
                     EVDFISPESNLFTLSWERKHSSMQKVTFEKVDSNYVAPLIDFKEQEEI"
     gene            18420..19256
                     /locus_tag="ACG8KR_000025"
     CDS             18420..19256
                     /locus_tag="ACG8KR_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HFQ0199436.1"
                     /translation="MIGLIYFVVIVLANTVGAISGMGGGVLIKPILDLIGAHSVAGIS
                     FYSTVAVFTMSIVSTVRQVSSGKSLNWQIVGWVSSGAVVGGVVGNIVFEVFLQLFENE
                     KHVQLIQIFLTVLTLVFAFFYTKHHQPKFHLTSWTWYLICGGVLGFLASFLGIGGGPV
                     NVSLLMLMFALPIKEATLYSLSTIFFSQLAKLVTIALTSSFMRFDLSMLFYVIPAAVI
                     GGLWGARFSRVLSPKKVTFIFQVIVLVVLLVNCYNAFVILQTLGESRLTQVKITETYC
                     SK"
     gene            19321..19755
                     /locus_tag="ACG8KR_000026"
     CDS             19321..19755
                     /locus_tag="ACG8KR_000026"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0199437.1"
                     /translation="MLREMRLEDAEALREINADQLGYDIPLTVTVRQMKKLLKEPEKN
                     FFLVYEDPTSKKAAGYVQAESYESIFSDPMFNIMALAVSKYAEKRGIGKALMTGVEEE
                     AKKRGITTIRLNSAEYRVEAHQFYEHIGYHSDKMQKRFLKIL"
     gene            complement(19823..21145)
                     /locus_tag="ACG8KR_000027"
     CDS             complement(19823..21145)
                     /locus_tag="ACG8KR_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HFQ0199438.1"
                     /translation="MAQKTTTATAVEEKKKEGFWSTKITGVSLPLYLIMVLVLIIAMA
                     LGKLPTNMIGPIAVLVIFGNLFHFIGTKIPIVKSYLGGGSVFAIFASAAIATFGILPE
                     YVVKSTENFVSNMGFWDFYIAALITGSILGMNRNLLMKASVRFIPVALISMVVSFFAV
                     GLVGMLIGNGFANSVLYISLPAMAGGVGAGAVPLSTIYANVLGTDASSIISRLIPATA
                     MTNVLAIIGAALVARAGQSMPKFNGNGKLMEKGDLGDGKPRTVKPSIPQLGVGLMVSL
                     TFFILGQIGNYFVPKVHAYAFMIIIVVICKIANLLPEYYEDAAIMFNNLIVKNLTAAV
                     LAGIGIALLNLNVLASALTWQFVVLCLTSVIAISVVSGFVGRLFGLYPIESTITAGLC
                     NNSMGGTGNVAVLSAANRMELIAFAQMGNRLGGAIVLIISGFLMQLLS"
     gene            complement(21299..22939)
                     /locus_tag="ACG8KR_000028"
     CDS             complement(21299..22939)
                     /locus_tag="ACG8KR_000028"
                     /EC_number="4.1.1.101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043883 - malolactic enzyme activity
                     [Evidence IEA];
                     GO_process: GO:0043464 - malolactic fermentation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malolactic enzyme"
                     /protein_id="HFQ0199439.1"
                     /translation="MLTGLNLLNNPFLNKGTAFTKEERAKYGITGMLPSTVQTLEQQS
                     VQAYGQYLSKQTDLEKRIFLMNLFNTNRTLFYKLMGEHLVEFMPVVYDPVVADSIEQY
                     NEIFLKPQDAAFLSIDEPENIKASLKNAADGRDIRLIVVTDAEGILGMGDWGVNGVDI
                     AIGKLMVYTAAAGINPAQVLPVSIDAGTNNEELLNNPLYLGNRHARVEGETYYEFIDQ
                     FVQSATELFPELLLHWEDFGRGNAAAILEKYEDQITTFNDDIQGTGIVVLAGVLGGLN
                     ISGEQLKDQIILTFGAGTAGVGIANILLDEMIRQGVPEEEARQHFYQVDKQGLLFEDT
                     EGLTPGQIPFARKRSDFTNADELTNLEAVVKAIHPTIMIGTSTQPGAFTEEIVKEMAA
                     HTPRPIIMPLSNPTKLAEAKAKDLIEWTDGKALVGTGIPAADVEYNGVTYQIGQANNA
                     LMYPGLGLGLIASTATRVNAEIISQASRALGGIVDVTKPGAAILPPVAKITEFSQTIA
                     ETVAKSVVAQNLNREEITDIKEAVESAKWVPEYKSLED"
     gene            23269..23739
                     /locus_tag="ACG8KR_000029"
     CDS             23269..23739
                     /locus_tag="ACG8KR_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4809 family protein"
                     /protein_id="HFQ0199440.1"
                     /translation="MKEVQLIRKSELSEGGCNACGVVEATSYTLKLDSNQAIISELTV
                     GGLVDSLALAEGFTGEDIYEMFSEVRQLKKGEKCIEVHHESPNVRFKRGDNEMIFKNH
                     VSDHTELYEIVNQILSGIFELGPYEFKEENGNPKLNEEWQETIETQRNNPHLFQ"
     gene            24368..25471
                     /locus_tag="ACG8KR_000030"
     CDS             24368..25471
                     /locus_tag="ACG8KR_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HFQ0199441.1"
                     /translation="MEKTYQAGTNEGIVDFINMEDLEIAASQVIPAGGYGYISSGAGD
                     LFTYQENERAFNHRLIIPHVLRDVELPDTTTHFDEEMLTAPIIMAPVAAHGLAHVKAE
                     KASAKGVADFGTIYTASSYASCTLEEIREAGGEKAPQWFQFYMSKDNGINLDILEVAK
                     RNGAKAIVLTADATVGGNRETDRRNGFTFPLPMPIVQAYQSGVGQTMDAVYKSSKQKL
                     SPKDVEFIAAHSDLPVYVKGVQSEEDVYRSLESGAGGIWVSNHGGRQLDGGPAAFDSL
                     QYVAEAVDKRVPIVFDSGVRRGQHVFKAIASGADLVAIGRPVIYGLSLGGSTGVHQVF
                     DFFKTELEMVMQLAGTQTVEDIKKIKLRENRFI"
     gene            25755..26435
                     /locus_tag="ACG8KR_000031"
     CDS             25755..26435
                     /locus_tag="ACG8KR_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HFQ0199442.1"
                     /translation="MKKNMIGTLLLSTLLLGGLATPAFAEGQATSKGDITFTEPTNTV
                     EPLNPTDPSKPVEPADPENPATGQTGSLTLDVVPELPFGTHEIESGTKTYQVDASKND
                     TPYLQVSDRRGVGADGQAQGWNVTVSVSDFVNGSQVLQGAELDFGTSTVKSTSDNEST
                     GPTSQTVTGLSKASAATPIFTAAKDQGLGTWLSVYDPANITLKVPKAAAGTFTADLTW
                     NLVAGPVA"
     gene            26525..27559
                     /locus_tag="ACG8KR_000032"
     CDS             26525..27559
                     /locus_tag="ACG8KR_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HFQ0199443.1"
                     /translation="MNQSKRLFVSFFSILSLFFIFPSISQAKDSAGDFGIKPVFPENQ
                     IDKAIGYFDLLVAPEQNQTLEVIISNSSDEERTFEVSVNPAVTSDGGTIDYSQKNPTL
                     DETLPFDVRDVLLIAKKEINVSAHAETTIPIEVKIPAKSFKGRVLAGIHVSPKEEAET
                     ENATEGAQIKNRIAYNLAVVLQESQETIEPDLKLLSGDLDEVNAKPTVQLRFQNPQPR
                     IISNLIFTSKIFYENQLYIENTSNEFLVAPNSNFHLNLDLAGDKAKAGDYRAEIIAKS
                     GDSNEWRFTQNFTIKKEKAQKVNENSVFAVQEQSFPWLYAGLVAAVLVLWLILLFLWV
                     HKKKKKGETT"
     gene            27573..27908
                     /locus_tag="ACG8KR_000033"
     CDS             27573..27908
                     /locus_tag="ACG8KR_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0199444.1"
                     /translation="MKTSILLGGIIASLLFFSWQEPVQADTKSSGTITFYDLSPVPHP
                     SGSVNGASKQQTQLPIYKESNQHGVFPETNEEKIRFLYLIIGIELWLLAWLLLWRRKE
                     KEDEHVATK"
     gene            27892..30372
                     /locus_tag="ACG8KR_000034"
     CDS             27892..30372
                     /locus_tag="ACG8KR_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0199445.1"
                     /translation="MLLQNKFSFFRSFFFTSLFVLSIFIFSPSAEAVGATPPAISYDP
                     YTYENIVPPNGGRSAVNGGKLVQLTGANQLPKLTATYLNDGSNDPWKGGTVRFHWESH
                     VKLTNVLDLVNITAQGFAFVAIINLPEHVSSSDVLKAIDWNNAYLYIAGAPIKVTSGS
                     LLSAGSHTLRFGMGSWDTNTLLADIIAIITRGEFNVDDIPLQFDVTANVAKMTENGDK
                     YDTSPNKILTKGKLQPSMSKKAVFSVDFYDSNNIIQDRMFNSILAPAAGRFYPKLTGG
                     NLNPGAYAFSASAEINTWNSYISTSDSTGKYNTFADTTEYVDGNNQNLPGSTNSRTLT
                     MGLTEGRFDQLPFSRFDRVVNYFTGKNVTQGAILTHTPASTSGLNQTANIVYSGKDAQ
                     GGLLSPVALKVLEQYKLDGTVLPTNLTADSQWGKLLSSASYQVTANWSATELKKGSIH
                     YRLYSKGTQKLATGWTDQLFQTIQSNNGGVNTATSTLPALASGQYYFDYRLVDDVLTN
                     MYPSFAYKWQSEQAGITTLPQITVANFPSVSADSQLKNLSRQPETGDPLSALSGDTIQ
                     ENVTFTLTKSGDAITDKKITISLPENTTYINGSLKLNGTTIADTGIQQGVSVPADLLS
                     KIGDTIHLTYNYQLNTVDTSVQSVSILTKAAVLSSNITLADGAKLPNPVVQTSAKTIL
                     VPKQELTLVNVPDDFTFGNDLPKPLKTSYYEAKGDFSFDVRDTRLPSTSPWQLTGTLT
                     SLFKNDQGQELSGTKLYFNHSGSKQLIQQGQNTLIYESDGTAKGEVLVDFPDTDGLLL
                     EVNSSTNAQPGATYQGMVTWELTAGPTS"
     gene            30428..31987
                     /locus_tag="ACG8KR_000035"
     CDS             30428..31987
                     /locus_tag="ACG8KR_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0199446.1"
                     /translation="MRRKLLLLKSEMLGGVDLELFSLLEKNEQRQIKLFQLLLEEKGE
                     ISVNNVLTRLSVDRATLKEDLFYLQANLENIKQLEIVIQEGNILLIRHGNIAAVDVYY
                     HCYLRYSTKYQILLYLLENGSFDRQKLVMELNISSATLSRRVKELNDSLNEFHLKIKS
                     GRLTGPESQICYFYFQLFWFGRPYLENKKEYSDLSGNTFLDLLQKEFSFTITEEGRLK
                     FEIWLSIMKKRMRCTKKSKPDYSNLPVGLFQTTQFLLDLHTFLTRYFFQYAFPWNQNE
                     TLVFYLFMISNFTLDTHNSHVQEFLISEKYKDDVVAQLNRTFKQSIEEYFSFYVFPEE
                     ISQKIALTVMQNHAHFAYFRGWMNIFGQEGLKQRLTEVEDKQFLVLCERLVRQTFAIV
                     DFTEVDEENARFELFARYASILRTLIKQLELRLQIAYDLPYERLMADIVKEIIQEQLL
                     NTIKYDLVEYEKEKQYDIILTSQLKEYPSQKSAKVFVFTSNEYNYDFPYLNQFLKECY
                     LEKLNLRMNKT"
     gene            31984..33348
                     /locus_tag="ACG8KR_000036"
     CDS             31984..33348
                     /locus_tag="ACG8KR_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HFQ0199447.1"
                     /translation="MKIVIIGASHAGITAALNLRKLQPKAEVLLIDEDHKDGLGYVSN
                     GINLYLKGKIRSLAEVAHNMRTLQESGAKLITEWRVTELDPDKHQLILASKEGKNETI
                     TYDKLIVATGSSPVTLYKQIEAENVYTYKNLVQSKQVLAALKEAKEVVIFGAGYIGLE
                     LADALRNKGYIIHLVDYMPNVLSRYFDKDMINSFQNQLQTKQINFYPNEFLIDWKKSE
                     EKVVSVQLLSQAIKADMVIFSAQTRPNTTLLKEKVALYEDETVIVNEYLQTSDPDIYA
                     IGDIVPVSFDKNKRHLFLPLVTRAVHMARAVALTLSGQPTAYDLRQKITAAVIIDYFL
                     GTVGLTEDEAPFLEQNTGSCSGEFDLFPQYDEENKTVNAKLIYHPDTLEIIGGQLISQ
                     EFLLSDLNLLADIVKHKTTIPQLAVEDFGFLSEHTSRFHYLNELAFKVLAKRANSNRV
                     DKRL"
     gene            33519..34589
                     /locus_tag="ACG8KR_000037"
     CDS             33519..34589
                     /locus_tag="ACG8KR_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0199448.1"
                     /translation="MFLALNEIMHSKLRYALVAGVMFLIAYLVFFLTGLAYGLAQDNR
                     TAVDKWEADSIVLSKDANSNLGMSMITKKIAEEVEGGKVAYLAQTPGVVTSKDSTEEG
                     KINVSFFGIDKNQFIMPNLVEGKAFDNDDEAVGDISLKEEYGLAVGDTVKLSGSDKTF
                     KLTGFTDHAKFNVSPVLYTTINAYQEIRFEKEDTSENARINAIVVRGKISNLPEDLEQ
                     IKISKFINELPGYNAQVLTFGFMIGFLIVIAAIVIGIFIYVLTMQKINIFGVMKAQGI
                     TGGFIARSVVAQTFILSFVGILLGLLGTVGTSLVLPDAVPFQSNWLFFGVISLLMLVV
                     AVLGALFSVRTIVKIDPLKAIG"
     gene            34604..35272
                     /locus_tag="ACG8KR_000038"
     CDS             34604..35272
                     /locus_tag="ACG8KR_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0199449.1"
                     /translation="MKAIEFKEVEKSFQDGDQVVKALKETTMSIDKGEFVAIIGPSGS
                     GKSTFLTLAGGLQTPSSGSVFINDQEFSNQKESERVKLRFKEIGFILQASNLVPFLTV
                     KKQLLLVDKIKHEHRQKEAKELFEQLGVDKLINKYPEELSGGERQRVAIARALYNDPS
                     IILADEPTASLDSEKAREVVDILAKESKDKNKATIMVTHDTRLIDKCDRIFMMEDGVL
                     KEKE"
     gene            35292..35870
                     /locus_tag="ACG8KR_000039"
     CDS             35292..35870
                     /locus_tag="ACG8KR_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFQ0199450.1"
                     /translation="MVHRYNKDTQQKILKEATDLFMSKGYLGTSTREIAQKVGITQPN
                     LYHYFGDKEKLYTAVLENHLKDVGKALREIVQTSETGFQVTLTKMAQFLIETHLVDLF
                     LMLHDLESNLSKETRDHLFFLWKKNYREPFEEIFSANQSVLRNGISKEIAARHFFLVL
                     APYITRSKRPVEEPLNVAQLIDLYLNGVLGEA"
     gene            36273..37184
                     /locus_tag="ACG8KR_000040"
     CDS             36273..37184
                     /locus_tag="ACG8KR_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFQ0199451.1"
                     /translation="MKKIIGAFVFAIIIIIICFQIDVFGQKKIVGQTVSSTNSTVTKT
                     KESFDDTEQETLIKIGKNDRTLYTDDSLTTPLATINGGELAEFLSETKNAYQIKTNDG
                     YTGYLALEDGTKVTKNIQTKPEELSDAVIVLDPGHGGNDTGALSNDEQTEEKDITLST
                     AIKVKKALEDAGATVYLTHTTDELVQLGDICDYSEEKKADVFISLHADSTEYANEATG
                     ITTYYYYGQEETLAQTIADSFTDLPLDSRGISTGNYQVLRENLQPSILIEMGYMNNDS
                     DLKELVTDSYQQQIAASLTQALTTYFQ"
     gene            complement(37440..38357)
                     /gene="rnhC"
                     /locus_tag="ACG8KR_000041"
     CDS             complement(37440..38357)
                     /gene="rnhC"
                     /locus_tag="ACG8KR_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HFQ0199452.1"
                     /translation="MSNVIIKATNDQLSQMKAYYRSYLLNKQIPYTSFAAKKDGTTIT
                     AYTSGKVMFQGTNAAIEADKWGEAQDKQAKPAKTASSLPKNISQLSVLGSDEVGNGSY
                     FGPLTVCAAYVKRDQLAELRKLGVRDSKELKDSQIIQLADILKKTIPYKLLVLSPEKY
                     NEIQPKYNAVRMKVALHNQAIHLLLKKIEPEKPEAILIDQFTPEANFKKYARLEKNQL
                     QEKLYFVTKGEQYHLAVAAASIISRASFLEELHKASAEAGITLPSGAGTKSDQVAAKL
                     LEKGGMPMLEKYAKLHFANTEKALKLLKK"
     gene            38559..38996
                     /gene="zapA"
                     /locus_tag="ACG8KR_000042"
     CDS             38559..38996
                     /gene="zapA"
                     /locus_tag="ACG8KR_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HFQ0199453.1"
                     /translation="MAHEKTRYKAVIANQTYTIIGRETKHHMDIVTKLINEQLAELKQ
                     LSPQMDNEQAAILMAVNALSDQLKKQERILELEEETAELKKKMIKFTELENRVKRIEA
                     IENEAREVLKENGQADHTIKNHVEAQQILNEKRKGQIKQKSSN"
     gene            39054..39601
                     /locus_tag="ACG8KR_000043"
                     /pseudo
     CDS             39054..39601
                     /locus_tag="ACG8KR_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629130.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0009403 - toxin biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
     gene            39716..42076
                     /locus_tag="ACG8KR_000044"
     CDS             39716..42076
                     /locus_tag="ACG8KR_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HFQ0199454.1"
                     /translation="MNNRILETLEFEKVKQMVRQFVVTAQGKEELAELVPVSEKQTIT
                     NWLQETEDGLKVQRLRGGIPIPKLENIRPHMKRIEIGADLNGVELAQVSRVLSTTSEL
                     NRFIDDLSDSEIEFARLYMWADQLVTIPVLSRRLKEAIDEDGRVTDDASPELKSIRQN
                     IRRSEQAVREQLDGIVRGKNAKYLSDAIITMRNDRYVIPVKQEYRGVFGGVVHDQSAS
                     GQTLFIEPKQVVDLNNRLRQYQIAERNEIQRILSELSAELVPHRQEIIHNAYVIGKMD
                     LMNAKARFGKEVKAIVPGISEDNHVVLKQARHPLIDQEKVVSNDITIGKDYQAIVITG
                     PNTGGKTITLKTLGLLQLMGQAGLPIPAGEESQIGIFEEVFADIGDEQSIEQSLSTFS
                     SHMTNTVDILSKVNEKSLVLFDELGAGTDPQEGAALAIAILDDLGKKSAYVMATTHYP
                     ELKVYGYNRANTINASMEFDVDTLSPTYRLLIGVPGRSNAFEISSRLGLDTEVIDEAK
                     QLMNDESQDLNEMITDLENRRKMAETEYLEMRHFVSEAQELHDDLKEAYSYFFEEREK
                     EMEKAKKKANEVVSEAEEKAEKIIADIRKMQQQIGQGNVKEHQLIDAKTQLANLHQEE
                     TLKKNKVLKKAKEQKTLKPGDEVLVTTYGQRGTLLRKNGNQWQVEIGILKMNVSEDEL
                     TPVAPQKEPTQRVIHAVRSESSSHVPNQLDLRGKRYEEALSEVDQYLDSAILAGYPQV
                     TIVHGKGTGALRKGITDYLKNHRSVKSFEFAPANQGGNGATIVKFK"
     gene            42163..42477
                     /gene="trxA"
                     /locus_tag="ACG8KR_000045"
     CDS             42163..42477
                     /gene="trxA"
                     /locus_tag="ACG8KR_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HFQ0199455.1"
                     /translation="MSQVITDKTFNEETDKGLVLIDFWATWCGPCRMQAPILDQLEQE
                     YDEEEFRIAKMDVDENPETPQQFGIMSIPTLMLKKDGQVVEKAVGVHSKEQLRQMIDQ
                     YL"
     gene            42659..44437
                     /gene="uvrC"
                     /locus_tag="ACG8KR_000046"
     CDS             42659..44437
                     /gene="uvrC"
                     /locus_tag="ACG8KR_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HFQ0199456.1"
                     /translation="MNERIKNKLALLPDQPGCYLMKDKNGTIIYVGKAKILKNRVRSY
                     FTGSHNTKTERLVSEIEDFEYIVTESNIEALLLEINLIKKNDPKYNIMLKDDKTYPFL
                     KITNEKYPRLVITRKVLKDKALYFGPYPDVGAANETKKILDRLFPLRKCKPSQTKEPC
                     LYYHLGQCLCPYYFDVDPKVYTKIVDEVKKFLNGGHEKIESEIHQKMEKAAENMEFEK
                     AAEYRDQIRAIETIMTRQKMTNTDLLDRDVFGYAIDKGWMCVQVFFVRQGKLIERDVS
                     MFPFYNEAEEDFLTYLGQFYQENEHFIPKEVLIPDTIDKPSVEALLATKVLQPKRGEK
                     KKLVELANKNASVALNERFDLIARKQERTIGAVERLGNAMNIPTPIRIESFDNSNIMG
                     TNPVAAMVVFIDGKPARKEYRKYKIKTVNGPDDYASMREVIYRRYSRVIREGLPLPDL
                     ILIDGGKGQVDVAKDVLENQLGIDIPIAGMAKNDKHKTNELLFGIDLAVVPLERNSPE
                     FFLLQRIQDEVHRFAITFHRQLRSKNSFASRLDEIEGLGPKRKKMLLKEFKSLKNITA
                     ATTEELQEIGLPKNVAQNVFDKLHQE"
     gene            44582..45481
                     /locus_tag="ACG8KR_000047"
     CDS             44582..45481
                     /locus_tag="ACG8KR_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0199457.1"
                     /translation="MLEVKDLVKTFGNYTAVDHVSFQIPDGKIMGLIGQNGAGKTTTF
                     RLILDFLTQDQGEVLWNGHHLGEKDYDVIGYLPEERGLYPKVTIQDQLLYFAELRGKS
                     RKEIEPKIDFWMEKFQVKGKKTDKVKSLSKGNQQKVQLIATLIHEPKLIILDEPFSGL
                     DPVNAELLKDGILELKKHGSCVIFSSHNMDNVEKICDHLIMLRNGKMVLNGKVHEIRE
                     SFGRTKLFLESGLSQQEVEEIEGVKKVILHEDNTLEITLEDPEAGKEIFTRATQFGYI
                     PMFNQQPPTLEEIFKLKAGEIDA"
     gene            45474..46736
                     /locus_tag="ACG8KR_000048"
     CDS             45474..46736
                     /locus_tag="ACG8KR_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0199458.1"
                     /translation="MRKFWIITKDVYLKNVKSISFLIMILVPFIMLGIIYVAGNFAQQ
                     NSDVNKIGVLADNEQISQQMSQVKTDDFEFKAVDSEEKAQKELSEEKIDAYLVVSTEN
                     DKINGMLYSENSLGQSTQLLIQQQLTGLQSMLRASQLGISPEEVASLSQQASLTRQKV
                     NFDSKGKMTVGEDNSTVQYVVSYVATIVLFIIILTYAQIIAQEIASEKGTRIMEVILS
                     STTAQKHFYGKLTGVLLVALTQMALYGIIFGIGYSQFKDMEMVKNILDGISLDNLIGP
                     FLWYSLLFMIFGILIFSVLAALCGSLVNKAEDTAKAILPVTYLSLGGYMLGLILGASD
                     PNNIVIRITSYIPFLSSYIMPVRLANETVGMGGALISLLVLVAVTFLLMILSANMYKS
                     NVLVYSEGGLWSSLKQSISIMRNERKKA"
     gene            46899..48797
                     /locus_tag="ACG8KR_000049"
     CDS             46899..48797
                     /locus_tag="ACG8KR_000049"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HFQ0199459.1"
                     /translation="MDRYLDLLSEKFPTTEDVITEIINLEAILQLPKGTELFLSDIHG
                     EFPAFDHILRIGSGNLKEKIKDLFSEQMTETEISQFTIFTAYPEYVLTTDWYKKQDKS
                     QLIHRLIDLLRFTTVKYTRSKVRKGLPKEYSYIIEELLYIDDRINGKKDYVKKIIEQL
                     VLMKEEEKFLTKLAQTIQKSVIDHLHIVGDIFDRGTQAAKVMDRIMDFSSVDIQWGNH
                     DILWIGAYCGSEACLLTLLRIAARYNYLFELEKEYGLNLRPLFSFAHQTYQKNPVFTP
                     KNRKDLSEREQEELEKIHQALSILQFKSEHQLLNRRPEFKMDDRKLLEKINYEESTIV
                     LEGQLYELKGTCFQTIQPDDPKKFLEEEQKVIDSLMYSFQHSLRMQKHMTFLIEKGGM
                     YLTNNQHVLFHGCIPVDEKGQLLAFELDQSYRGKELLGFFEKQITESAKDLTANEDLA
                     TDLIWYAWSGPFSPLFGKSKMATFERYFLTEPHTHVEKSNPYYHLRDEEWFSEKILAE
                     FDAKEEGSAIINGHTPVKVKKGESPIKANGKAFVIDGGLSKAYQKTTGIAGYSLLCNS
                     YGFQIVTHYPFLPIEQQFAKKSDQTNVKKVIEQQLPRKLNRNTTKGMELQQQITQLQR
                     LLDYRKDD"
     gene            complement(49096..49815)
                     /locus_tag="ACG8KR_000050"
     CDS             complement(49096..49815)
                     /locus_tag="ACG8KR_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HFQ0199460.1"
                     /translation="MKPIIGIAGNQLIRATDTFQGNQVSYTPQGFVDAVLDAQGLPLI
                     LPVMSPDSAPQLIEKIDKLILAGGQDVSPQLYMEDPHPKLTETNIQRDQFEQALILEA
                     LKQRKPIFAVCRGLQLLNVVLEGTLYQDLSLYPKWSVKHEQQPTAPQFATHEVKIVSD
                     SLLSDLLPDSYFVNSYHHQAVKDLSPLLKAIAFSNDGLIEAVQSKDNMHKILGVQWHP
                     ELSHRVDSSEQRLFDFFVNEF"
     misc_feature    50005..50052
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF03071"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="sodium ion sensor (DUF1646 type); this
                     cis-regulatory element may regulate processes involved in
                     with the transportation of sodium ions; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF03071"
     gene            50152..50484
                     /locus_tag="ACG8KR_000051"
     CDS             50152..50484
                     /locus_tag="ACG8KR_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0199461.1"
                     /translation="MKQVLEALEQIRKAEEENEKRARTLRQQLKTYAEEKEQELEIIQ
                     TNSRQQLKQLVEEKEQFEKQELEKEKMKLKAEADKVQATLKDQYQKHQSQAIGAIIER
                     VKETYGRH"
     gene            50471..52474
                     /locus_tag="ACG8KR_000052"
     CDS             50471..52474
                     /locus_tag="ACG8KR_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HFQ0199462.1"
                     /translation="MAVTKMEKVTLISDKRNQERILQAVQGIQNVEIRDLYQNRTNNQ
                     WVEKYFPDTTIIDKEAKLSALGNRLKDIQEAIQFIQHHGDSRQKKLHLKRQELSLQDL
                     EANYSEENFMNKLEEVLDLKEKWEQLTEKRDRLEEEEEWLLHWQQLDIAPSMYASKTT
                     IFEMGTVSAANFESFKEELSKLDDVYMEEIDYTPKQVYLSYVALKKSVAAIEEIAGKY
                     GFNKETYPYEQNPKEKLQETKQQLSIVHEQQKKITTALGQCSGYIQEFEWVEEVTLAV
                     AEREKVKERFIHATYLIVLQGWVDAEEKQNLMHVLNETVSEEDIYVSFETPNSEEIQQ
                     EVPTKLKNHPLIEPFELLTEMYSLPKYEEVDPTPWMTPFYLVFFGMMVADVGYGLLML
                     IGTILAQKLFVLPRGMKRFVKFFEILSVPSIIWGLIYSSFFGQALPKELFGIRLPFPI
                     LSTTDDVNTILILSVIFGLIQILVGLFVAAKENIRRKDYLSAVSDGFAWQGILIGIVI
                     ALIGVMLIKNTAFVYLGAGLSIIAALCILIVPIIQSPSKVKGAAKGAYNLYGLTGYIG
                     DLVSYTRLMALGISGGSIGAAFNMLVAFMPPAARFSVGILLIIALHALNMFLTLLSAY
                     VHGARLQYVEFFGKFYTGGGRAFDPLKTAEKYVNINHKKKNNK"
     gene            52493..52963
                     /locus_tag="ACG8KR_000053"
     CDS             52493..52963
                     /locus_tag="ACG8KR_000053"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HFQ0199463.1"
                     /translation="MMDYLISQNGGMVFAVLAMATATIFSGIGSAKGVGMTGEAAAAL
                     TTSQPEKFGQALILQLLPGTQGLYGFVIAFLIFINLGNDMSVVQGLNFLGASLPIAFT
                     GLFSGIAQGKVAAAGIQILAKKPEHATKGIIFAAMVETYAILGFVISFLLVLNA"
     gene            52984..53568
                     /locus_tag="ACG8KR_000054"
     CDS             52984..53568
                     /locus_tag="ACG8KR_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit E"
                     /protein_id="HFQ0199464.1"
                     /translation="MNAIEKIISQMNEAAEQERAALEQEERMKIDQNFEQKRTQIETE
                     HQKQKEKQIELLEKKYRQLRNRQQVEVRQENLNAKQEFLRRLFADAIAEMENWDESEQ
                     IQFIKNSLYSLPLTGKVTFIAGEKSAAYLSQTLLDEWNNELPFMMVLSDETVADQAGF
                     LINDQGVQYNFLFSSLVQDIQGTMSFEIANQLFE"
     gene            53583..54569
                     /locus_tag="ACG8KR_000055"
     CDS             53583..54569
                     /locus_tag="ACG8KR_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046961 - proton-transporting ATPase
                     activity, rotational mechanism [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HFQ0199465.1"
                     /translation="MEYHELNPLIRGRELELLSNETFERMIQTNSVEALGEILKSTIY
                     SPYIYDGYEYDFEQNLLKEQSRLFRWLKESAPEPEIVWIYTMRYTFHNLKVLTKAEMT
                     NQNLDHLYIDDGFYSLETLKDAIHTQVSNELPESIMDSIREVRDYFEESTILQGIDVI
                     YDRQFLTEQRRLADKLGYQELLTEVIAFIDLTNITTMARGILQKRTYGFMTTVLSSSG
                     SIEKEVFLNFVDKDMEDYTNFLLETDYADFLRPVIREGNLDLVKLEQLKDDYLSSLYQ
                     EAQTQAFGPLPLLAFLNAKEVESKNLRLLVIGKRSHFEPETIRKRVRQVYDA"
     gene            54559..54870
                     /locus_tag="ACG8KR_000056"
     CDS             54559..54870
                     /locus_tag="ACG8KR_000056"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HFQ0199466.1"
                     /translation="MTHKIGVVGDKDSVLPFKLFGFDVRYGTTKQTVRQAIEEMAKNE
                     YGVIYVTEQCAALSPETIERYKEQMIPAIVLIPNHQGSLGIGLEEIQKNVEKAVGQNI
                     L"
     gene            54892..56673
                     /locus_tag="ACG8KR_000057"
     CDS             54892..56673
                     /locus_tag="ACG8KR_000057"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HFQ0199467.1"
                     /translation="MQIGKIIKVSGPLVMAENMSDASIQDMCLVGDLGVIGEIIEMRG
                     DVASIQVYEETSGIGPGEPVRSTGEALSVELGPGIISQMFDGIQRPLDTFMEITQSNF
                     LGRGVQLPALDHEKKWWFEPTVEAGETVSAGDVIGIVEETKVIKHKIMVPNGIKGTIK
                     TIEKGSFTIDETVCVIETENGDKNLTMLQKWPVRRSRPIKEKLNPDAPMITGQRVIDT
                     FFPVTKGGAAAVPGPFGAGKTVVQHQIAKWADVDMVVYVGCGERGNEMTDVLNEFPEL
                     IDPNTGESLMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYDVAIMADSTSRW
                     AEALREMSGRLEEMPGDEGYPAYLGSRLAEYYERSGRVIALGSEGREGSITAISAVSP
                     SGGDISEPVTQNTLRVVKVFWGLDSSLAQKRHFPSIDWIQSYSLYSTEVGKYMDHILQ
                     ADWASMVTEGMRILQEEQQLNEIVRLVGIDSLSDNDRLTLEVAKSIREDYLQQNAFDD
                     VDTFTSREKQFKMLKMILTFGEEGRRALSLGAYLSEIMDGTVSIRERIGRSKYIPETE
                     LEKLDQIIGEIKQTMQTIVSEGGMSND"
     gene            56666..58042
                     /locus_tag="ACG8KR_000058"
     CDS             56666..58042
                     /locus_tag="ACG8KR_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HFQ0199468.1"
                     /translation="MIKEYRTIGEVVGPLMAVEKVAGVKYEELIEVRMQNGEIRRGQV
                     LEVQEDKAMVQIFEGTSGINLRDSSVRFLGHPLELGVSEDMIGRVFDGLGRPKDNGPE
                     ILPEKHLDINGEVINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHKEL
                     AAQIARQATVLDSSDDFAVVFAAIGITFEEAEFFMEDFRQTGAIDRSVMFMNLANDPA
                     IERIATPRMALTAAEYLAYEKGMHVLVIMTDMTNYAEALREISAARREVPGRRGYPGY
                     LYTNLATLFERAGRIRGLKGSVTQIPILTMPEDDKTHPIPDLTGYITEGQIILTRELY
                     KSGIQPPIDVLPSLSRLKDKGTGPGKTREDHAATMNQLFAAYAQGKQAKELAVVLGES
                     ALSDVDKIYAKFAERFEKEYVNQGFYTNRSITETLDLGWELLSMLPRTELKRIKDDML
                     DKYLPEGK"
     gene            58049..58684
                     /locus_tag="ACG8KR_000059"
     CDS             58049..58684
                     /locus_tag="ACG8KR_000059"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HFQ0199469.1"
                     /translation="MARLNVNPTRMELTRLKKQLTTATRGHKLLKDKQDELMRQFILL
                     IRKNNQLRQEMEESMTEAMSDFVLANASITEPFIEELFVLPAENVDLSIVDKNIMSVK
                     VPVMNFEYDEQLTDTPLEYGYLNSSAELDRSIDRFTTLLPKLLELTEIEKTCQLMASE
                     IEKTRRRVNALEYMTIPQLEETIYYIRMKLEENERAEVTRLIKVKNMGTTD"
     gene            58714..60069
                     /locus_tag="ACG8KR_000060"
     CDS             58714..60069
                     /locus_tag="ACG8KR_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HFQ0199470.1"
                     /translation="MIKQSKQIKKRLPAVQLLALGFFLLILVGGGLLTLPFFSNSGHG
                     TNFIDALFTATSAVCVTGLTTLNTAEHWNSAGQFLIMLLIEIGGLGFMMVPILCFALI
                     KKKVSLSTRIILKEALNLEEVSGVMKLMLYILKLAFVIQLIGTVALSFVFVPEFGWLK
                     GIWYSLFHAISSFCNAGFDLLGDSLANHQQNVYLIMVVSALIISGGLGFIVWRDLLEY
                     HQKKKMTIHSKIALTVTGTLLLGGFVVFFFTERNGATLVEGNMFQRLANTFFMSVTPR
                     TAGYYSVDYLHMSHAGLILTMFLMYIGGTSGSTAGGLKTTTLGVLLIQMRSMLKGRTR
                     AEAFGRTIRQGAVLRALTLFFVTLTLCVVAIMILSVTETIPKTSGIEYIAFEVFSAFG
                     TVGLTMGLTPDLTAIGKLIIIALMYIGRVGVLTVVFSLMTKAQRTQSKYKYPEESVMI
                     G"
     gene            complement(60261..61655)
                     /locus_tag="ACG8KR_000061"
     CDS             complement(60261..61655)
                     /locus_tag="ACG8KR_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFQ0199471.1"
                     /translation="MPINSYDNYPLTWRPDKQKLKRPYYRSLIQQLEQDILKNKLQKN
                     TQLPSQRELADYLDLNFTTVGKAYKYGIEKGLLYTNIGSGTFISPNVFESITISTNEV
                     TEHIIDFGLVSSFEECNKFVVPFIQSTSNNPNLVGLLNYQDPKGTNFQLTTAANWLKT
                     QGVIPSLESLSIVSGVQNGLAITLSALFSPGDRIATDRYTYANFIELAQLYHLEIVPI
                     DFDEQGMRPDILIQECRKKKIHGVFLMPSCNNPIGFQISHKRREQLAEVIEKEGLWVI
                     EDDIHSFLTTYYQHTIIPPFQNLLPNQTIYLAGMTKFICSGLRVAYLVVPVHVQSKIE
                     QAIFNINVKTSGLDVEVITQILHSNEANRILETKLSLTKRANQLFDSLFKVEKPTNRV
                     PYYRILPVDPEIDPKIIENDFLANGIRLYHSRRFTTRAQPDAFVRISLSSNNLEILAS
                     GLSIVKEKIKKYEL"
     gene            61747..62718
                     /locus_tag="ACG8KR_000062"
     CDS             61747..62718
                     /locus_tag="ACG8KR_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HFQ0199472.1"
                     /translation="MKIINFEEVVASFSFEEAIQVMKKCFFDYESGKISQNPRLVEIL
                     PDGKGKNMFAMMPAYLGENRYFGTKVITAFPDNHQYHLPSHLGEIFLFDAQNGLPKAL
                     INANAVTWIRTAAVSALATDYLANPHAETLALIGAGQQALSHLTAISTIRPITTVFVY
                     DILEERRNTFIAKAKKQYPKITFINAISVEDAVRPAEIICTLTSSKEAFLQKEWVRED
                     AHINAVGTFTPDTREISTSLMETSQIYVDDYSAALTESGDLLIAMAEGDLEESCIVGT
                     LGELVSEKKTFYKGESRPTIFDAVGLAIEDLCCAEYIYNKLEKGALK"
     gene            62715..63497
                     /locus_tag="ACG8KR_000063"
     CDS             62715..63497
                     /locus_tag="ACG8KR_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HFQ0199473.1"
                     /translation="MNLDYYVVDAFADEVFKGNPAAVYVLEEWLPEGTMQKIAIENNL
                     SETAFTVKKNQEFELRWFTPDREIDLCGHATLATAFVLFNYYKIPDETIKFSTQSGNL
                     FVTRIKDYYYMDFPSIMPKKVPILSEYEEAIGAKIKEAYLARDLFFVLEDEKTVAKLQ
                     PDFTAIKNFELGIGVIVTAESVQKDFVSRTFFPKLTINEDPVCGSAHSNLIPYWAERM
                     NKTKLSAYQMSSRGGDLECELKEDRVIIGGKAVLFSKGTSFI"
     gene            complement(63666..64037)
                     /locus_tag="ACG8KR_000064"
     CDS             complement(63666..64037)
                     /locus_tag="ACG8KR_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFQ0199474.1"
                     /translation="MKMAHTCVRVKDLEASIEFYQKAFGFEESRRRDFPENKFTLVYL
                     TLPGDDYELELTYNYDHGAYDLGDGYGHIAIAADDLEKLHEQHKAAGLEITDLKGLPG
                     TAPSYYFVIDPDGYKIEVIRG"
     gene            64171..64614
                     /locus_tag="ACG8KR_000065"
     CDS             64171..64614
                     /locus_tag="ACG8KR_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HFQ0199475.1"
                     /translation="MDKPIFGEKKQFADYQTRYAAYIVIERDQQIAVIEAPNGAFFLP
                     GGEIEGEETKEEAIVRELIEEMGIVAEISSYLGEADEYFYSNYRATYYYNPGYFFVAD
                     SWKRIGEPTEKTNKIWWVSPQEALVKLKRGSHRWAVERWIEKNKN"
     gene            complement(64657..65466)
                     /gene="yidA"
                     /locus_tag="ACG8KR_000066"
     CDS             complement(64657..65466)
                     /gene="yidA"
                     /locus_tag="ACG8KR_000066"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HFQ0199476.1"
                     /translation="MSIKLVAIDIDGTLLNEKREVTKEVKEAIAAAVAKGVSIVLCTG
                     RPLPGVQEQLNELNLFQDNDYVITYNGALVQQTKSGKIIARHGLTHEDFLEIEVMARR
                     VGSHLHSIDDKAIYTPNRDISAYTIHEASLVKMPLKYRTPEEMTPDMNIVKMMMIDEP
                     EILDAAIARLPQTFRDKYTTVKSAPYYFEVLNKEASKGAAVANLAQHLGIDQDEIMAI
                     GDNENDLSMIEYAGLGVAMGNAVSLVKEAANVITSSNDEHGVAEAIKKYVL"
     gene            complement(65485..66852)
                     /locus_tag="ACG8KR_000067"
     CDS             complement(65485..66852)
                     /locus_tag="ACG8KR_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFQ0199477.1"
                     /translation="MTTPYKYQMLPMEKVFRDPVHNYIHVQHQVILDLINSKEVQRLR
                     RIKQLGTSSFTFHGAEHSRFTHSLGVYEISRRICDIFSRNFSKEKIGNGGWDDQERLV
                     TLCAALLHDVGHGPYSHTFEHIFHTNHEAITVEIITSPETEVYQILNRVEEGFPEKVA
                     SVIQKTYPNPQVVQMISSQIDADRMDYLLRDAYFTGTEYGTFDLTRILRVIRPYEGGI
                     AFSMSGMHAVEDYIVSRYQMYVQVYFHPVSRGMEVILEHLLHRAHELYQEDASSFELH
                     SQLLLPFLDGSFTLEEYLKLDDGVLGTYFTQWSESADPILNDLAKRFLNRKPLKSATF
                     SGNRDSKLVQELTLLVEKVGYNPVYYTAVNSSYDLPYDFYRPEQGRHRTQIEILRNDG
                     TLIELSQVSQLVAALAGQEQGDERFFFPKEMVDPSLRDHYDLFDETYQEFASHIRNGA
                     LIEIN"
     gene            complement(66836..67666)
                     /locus_tag="ACG8KR_000068"
     CDS             complement(66836..67666)
                     /locus_tag="ACG8KR_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HFQ0199478.1"
                     /translation="MNPAIRLDQHIYQKNDFSPFALTDILTEYSKEHQQIFLHFWQYD
                     CTVILGMKDVRTPFLSDGIQTLKDAGYAPVIRNSGGLGIVSDEGILNISLIFPQSDTK
                     KITIDDAYEQMLLLTKYAFPKAPIQAFEITHSYCPGTYDLSIHGKKFAGIAQRRIKNG
                     ISVMMYLSVNGNQEKRGQVMKRFYQSALKEDFGTNGYPAVDPATMATLEELLDQPLFV
                     KEVKEAFAKAFEKLYSASDLQDSTDWLKKEIQLEQWNNQIQRMEQRNNIKELHYDNPL
                     "
     gene            67829..68266
                     /locus_tag="ACG8KR_000069"
     CDS             67829..68266
                     /locus_tag="ACG8KR_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HFQ0199479.1"
                     /translation="MELSQGTPISIKLRTKVKQQGEVQDFYFDLKGQMVTIGDTLYIR
                     YKEIQEETGDEIPVTIKLVPDGHVQLIRAGEMRMRLKFGYKERLETSYRTPYGMIQIA
                     TFTKELHVSLKDRPTGGKVRIDYDLFMGPEKIGEYYLTLDFTA"
     gene            68342..68962
                     /gene="rpoE"
                     /locus_tag="ACG8KR_000070"
     CDS             68342..68962
                     /gene="rpoE"
                     /locus_tag="ACG8KR_000070"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HFQ0199480.1"
                     /translation="MEISVFEGANKSELSMIEVAHAILEQRGDVMDFSDLANQIQNYL
                     GKSDSEIRDSLAQFYTDLNIDGSFISLGDNRWGLRSWYAIDSIDEEVNHGMDEEDEDT
                     PRRRKRKKVNAFINDDEDAIDYNDDDPEDTDLTEEDDDDLFDDDDDEDEEIAAYNSDL
                     QEIGADDPTDDEEDLPGNIEEDLSIIDEDDDDEDYEEEEFSDSEKE"
     gene            69151..69657
                     /locus_tag="ACG8KR_000071"
     CDS             69151..69657
                     /locus_tag="ACG8KR_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HFQ0199481.1"
                     /translation="MIFTGYKKKRQIEKYRHLLNGLIETDYQKMFRITMNYVHDKEEA
                     LDVMQDSFHKALNYFEKEKEIEHFSAWFYRILIRTALDSWRRQKRTPVDLFEIQDIPQ
                     QLPLETSVAELHMILDKIDSPEKEILILYFFEGFRLKEIATILDMNENTVKTKMYRSL
                     NALRQILE"
     gene            69674..70573
                     /locus_tag="ACG8KR_000072"
     CDS             69674..70573
                     /locus_tag="ACG8KR_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsiV family protein"
                     /protein_id="HFQ0199482.1"
                     /translation="MYSQKENLKKLKKTYKEQAIPKGLKEEIRQRFIIEEQSFIKAKR
                     RKRRLQGFGVSLAIVMITTSSLLFNSQVRSFAEDLPILGSVIELILGERFTDRSEKID
                     IQVPKINTQNEKENKTIHGLNQKYFREGQADFEKAEKEYGNFETDHYQVLGDYQKIVD
                     DNRFLVIERQITQTAADSHVEKRYDTIDKKNSVQLSLPLLFKDDTYLSVLTKEVKRQM
                     AEQVKEDPSKYYWTEQDIQEGTIEKPTLVTPTRSFYLNKEHQLVLTFSQYEIAPGYMG
                     TPEFVIPKSVTKTILASEDYLDH"
     gene            70660..71544
                     /locus_tag="ACG8KR_000073"
     CDS             70660..71544
                     /locus_tag="ACG8KR_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFQ0199483.1"
                     /translation="MKRNYLVISIIALSVLLFFEITSVASLITEVKGQTHSSEHSSQS
                     FKQSEQSTIKSQSTETANVKEAIQESAEKVQSQSSQSTQDSTKTSRDPAVKSVAISFD
                     DGPGATTTPQLLRILKEKNVHATFFVLGENTAQHPEIVKQTAEAGHEIGNYTYDHQNL
                     ATLSAQSMTEEVTKADTEIKKVTGKTPTFVRPPYGSVTNVGATIIQRPIIEWSVDSED
                     WKTRNPDLILQKIQATVYDGAIILFHDIYPETIRAVPQVIDYLKEQGYRITTVGDLLG
                     HPTAVENYYGRNDHRPVQ"
     gene            complement(71736..71807)
                     /locus_tag="ACG8KR_000074"
     tRNA            complement(71736..71807)
                     /locus_tag="ACG8KR_000074"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(71772..71774),aa:Arg,seq:ccg)
     gene            71867..73201
                     /gene="rpoN"
                     /locus_tag="ACG8KR_000075"
     CDS             71867..73201
                     /gene="rpoN"
                     /locus_tag="ACG8KR_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HFQ0199484.1"
                     /translation="MKFEQNFSQKQQQTQKLAMTQQLQQSIQVLQYNSEELLAFVENQ
                     AMENPLVEVVEPEWQPDYIKASSSSYEGEETNYLNQIPDTKGSLFDSLIEQVHLNYRD
                     TFLRKIVLYLVEYIDLNGFLTISLEEAGKEIGATPIQMLDALTLIQQLEPAGVGARSL
                     QECLMLQTERDDYAPELAYIVLEECFEELVERKWKEIAQRFSVDLHAVQQIFDYLQTL
                     SPSPGRIFDRSSELYIIPDVRVLVDDDKNVQVISNRKNQPNIRFQESYFKQMSQQADK
                     ETENYLKERKQEFEWLKKTILQRGDTILRVAQVIVSRQKAFFIDKERPIKPLTLKEVA
                     TEIDVHESTVSRAVNGKYLETDFGVFELKKFFTTRIPTNSTEQTEDLSADTAKKKLQE
                     LVDQEDKNKPLSDQKLVELLKKDEIAISRRTVAKYRDLLGIPSSSKRKRYDN"
     gene            73350..74441
                     /locus_tag="ACG8KR_000076"
     CDS             73350..74441
                     /locus_tag="ACG8KR_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="central glycolytic genes regulator"
                     /protein_id="HFQ0199485.1"
                     /translation="MGRFKSTLDVKCPTKEIAMLDEIKMIESVAPDIIEVLQERYKIL
                     RNIYWMQPIGRRSLSESMGITERVLRTETDLLKNLNLIDTSKSGMTLTTKGEEVYQSL
                     ENFMDQLLGTHQTEQQLAEYFGNQRCIVVSGNSEEQTKVADAFGEALSEALDRLLPEG
                     ENIIAVMGGTTMAVVAEQLSNLENKKRHNLFVPARGGIGEAITVQANSVSARMAAKAN
                     GNHRALYVPEQLSLATYNSLLNEPSIQEVLNLISEANCVIHSIGRALHMAARRKMTEK
                     ELVMLKQANAVAESFGYFFNEKGEVVYKVPRIGIELQDLEKVPIIMAIAGGKSKAKAI
                     RAYMKNAPKQTWLITDEAAANEILKGVTL"
     gene            74482..75483
                     /gene="gap"
                     /locus_tag="ACG8KR_000077"
     CDS             74482..75483
                     /gene="gap"
                     /locus_tag="ACG8KR_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFQ0199486.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIQDVDGIEVVAINDLTDAKMLAHL
                     LKYDTTQGRFNGTVEVHEGSFNVNGKEVKVLANRNPEELPWGELGVDIVLECTGFFTS
                     KSAAEKHLTAGAKRVVISAPGGNDVPTIVYNTNHETLTGKETVISGASCTTNCLAPMA
                     KTLNDKFGVVEGLMTTIHAYTGDQMTLDGPHPKGDFRRARAAAANIVPNSTGAAKAIG
                     LVIPELNGKLDGAAQRVPVPTGSLTELVTVLEKEVTVDEINAAMKEASNESYGYNTDE
                     IVSSDIVGMTYGSLFDATQTKVMTVGDKQLVKTVAWYDNEMSYTAQLVRTLEYFANL"
     gene            75593..76783
                     /gene="pgk"
                     /locus_tag="ACG8KR_000078"
     CDS             75593..76783
                     /gene="pgk"
                     /locus_tag="ACG8KR_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005228679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HFQ0199487.1"
                     /translation="MAKKTVKDIDLKDKKVLVRVDFNVPLKDGVITDDTRIKAALPTI
                     NYVLEQGGKAILFSHLGRVKTEEDKEGKSLAPVAKRLGELLGKEVTFVPETRGEQLEE
                     AIRNMNDGDVVVFENTRFEDIDGKKESKNDTELGKYWASLGDVFVNDAFGTAHRAHAS
                     NVGIASTGIPTVAGFLMEKEIKFIGEAVEEPKRPMIAILGGAKVSDKIGVIENLIPKA
                     DKILIGGGMTYTFYEAKGIKIGNSLVEADKVELAKELIEKAGDKLVLPIDNVCAPEFS
                     NDVETQVIEGDIPDGLMALDIGPASVKLFADTLQGAKTVVWNGPMGVFEMSNFAKGTI
                     GVCEAIANLEGATTIIGGGDSAAAAEQLGFADKFTHISTGGGASLELLEGKELPGLAA
                     INDK"
     gene            76887..77642
                     /gene="tpiA"
                     /locus_tag="ACG8KR_000079"
     CDS             76887..77642
                     /gene="tpiA"
                     /locus_tag="ACG8KR_000079"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HFQ0199488.1"
                     /translation="MRKPIIAGNWKMNKTASEAKEFAEAVKNKIPSNDKVDSVIGSPA
                     LFLQELMTSAEGTELKISAQNCYWENSGAFTGETSPAALADLGVHYVIIGHSERREYF
                     HETDEDINKKAKAIFANGMTPILCCGESLETYEAGKTAEWIEGQITAGLADLSAEQVS
                     NMVIAYEPIWAIGTGKSADAQIADEICGVVRQTVEKLYGKEVSEAVRIQYGGSVKPEN
                     IAEYMAKENVDGALVGGASLQADSFLSLLDAVK"
     gene            77758..79056
                     /gene="eno"
                     /locus_tag="ACG8KR_000080"
     CDS             77758..79056
                     /gene="eno"
                     /locus_tag="ACG8KR_000080"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000015 - phosphopyruvate hydratase
                     complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HFQ0199489.1"
                     /translation="MSIITDVYAREILDSRGNPTIEVEVYTESGAFGRGMVPSGASTG
                     EYEAVELRDGDKARYGGKGVTKAVDNVNNIIAEAIIGYDVRDQMAIDKAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYHYLGGFNTKVLPTPMMNIINGGSHADNSI
                     DFQEFMIMPVGAPTFKEALRMGAEVFHALASILKARGLATSVGDEGGFAPNLGSNEEG
                     FEVIIEAIEKAGYVPGKDVVLAMDAASSEFYDKEKGVYVLADSGEGEKTTDEMIKFYE
                     ELVSKYPIISIEDGLDENDWDGFKKLTDVLGDKVQLVGDDLFVTNTQKLSEGIEKGIA
                     NSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTISDIAVATNAGQIKT
                     GSLSRTDRIAKYNQLLRIEDQLGEVAEYKGLKSFYNLKNK"
     gene            79455..80594
                     /locus_tag="ACG8KR_000081"
     CDS             79455..80594
                     /locus_tag="ACG8KR_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HFQ0199490.1"
                     /translation="MKKEEKIAILQEIIRIKSVNGNEGEVAAYLNKLLAKHDITGEIV
                     SYRDGRDNLIARYQKGQSGKVLGLSGHMDVVAAGDESSWTYAPFAAEIHGNRLYGRGA
                     TDMKSGLAAMVIAMIELKESGKPFNGTVKLLATVGEEVGELGGEQLTKAGYVDDLDAL
                     IIGEPTNYSLMYTHMGSINYTVTSHGKEAHSSMPDQGYNAINHLNEFITKANAEMNHL
                     AETIENPVLGKTIHNVTLISGGNQVNSIPSHAQLQGNIRSIPEYPNDKIIALLQSIVN
                     ELNQETDYHLELMIDYNKIPVKADPDSPLIHCIQQQFSQPLPLVGAAATTDAAEFTKA
                     NHSFDFVVFGPGVVTLPHQVDEYVEIDNYLDMIEKYQGIILSYLA"
BASE COUNT        27076 a        13724 c        16814 g        22994 t
ORIGIN      
        1 atatatttca attatttaaa agagatcaaa cctattttaa taaagttgaa tgacgagaaa
       61 aatgaattac tactcaatac caaatttcag ttaattaatt cagatggaag ctcaagtgat
      121 atgctttttt atgagtttga tggtgagaat tatcactgct tcaaccgaaa ggacaacagt
      181 atatctatgt atgctgacat aaaaagagaa gttaaaaaaa tattaaaagt agaagaacaa
      241 tactataaag aaatagaaaa aagaaaccag taattaacac agttatcaag gtaaagaaaa
      301 agttctcttt ctgtttgcta gttgtggctt atgtagcaaa tatcaagtta ataggaaaat
      361 taaaattgtt gaaaggaaag aagttgatga catattagta agcaagttgt tgaatattta
      421 tattatccta aaaaaggaag aatggaaaat atcgaagaaa cctttctgaa agtgttgtca
      481 ccatatatta gtgattctat cttaagattt caggagcagg aaaaaaatta taaatttggc
      541 tttgacataa agaactataa tgattcgtta cacgtagtat ttcaatataa taagactggt
      601 aagataggaa taaaagaggt acatatattt caaattttcc tagctaaggt caatattgaa
      661 gcaagtaaat caaaattaaa ctacactacc cttttagata caaatgcgtt gtttttttcg
      721 aaaaaactat taccgtattt gcatagttac gaatgggcat taagaaaatt aatctattta
      781 ttagctccgg catatttttc tgataactgg gcagaggaat caattccaaa tgaaatcatt
      841 tcgtcaataa aaagaaaaca aatacaagca ataggaaagt ttgactcaaa gaatctttta
      901 caatggatag atttatatga ttttgaagaa tatctctttg gagaaaatta tattttagtt
      961 acacaagaaa atgaagagaa tattgtaagg tataaagaaa tatctcagga tcaattattg
     1021 aagttatttc aagataacag tcacactata tctgattcat attctttgtg gagtgaagta
     1081 tttagtaaat atatggatgt taagctagaa gaaattcaaa cagacatgag tttaatcaga
     1141 gaaggtagga atattgtaag ccataacaaa gaaatagaaa tggctttata ccaaaaatta
     1201 ataaagaaat tgaagagata tacaaatcaa ctagagaaag cttttcaaaa aatattggct
     1261 ggagatattg ctggtgagga ccttagtgat atgtcagatg attttgaagg atatattgga
     1321 aattatttta gtaatatcga tttagcaggt gttactaaat tgtcagagtc tctatcagca
     1381 atgaaagtat taaatacaga ttccatgaaa aagtcgatgg agtcaatagg tcaaacatta
     1441 tcagtccagc aagaaattat gcagaacttt gatactgaag gtatcagaag ggcaacagag
     1501 ggaataaatc aatctctaca atcaataaaa atattaaata cagattccat gaagaggtcg
     1561 atagagtcaa taggtcaaac attatcagct ttatcagctc agcgagcaat catgcaaaac
     1621 tttgatacta aaggtatcag aagtgcaaca gagggaataa atcaatctct acaatcaatg
     1681 aaaatagtaa atacagattc catgaaacga tcgatggaat caattggtaa aatttcatca
     1741 acaaaccaag tattgaagtc agagtaaata aaacaggaac gatggcaatc atgttaccac
     1801 acgaaatgct atcatttgta gcaagtattg gatttagagt aattattata gagaggatga
     1861 gataaaatgg ttcaaggact ttttggtgga gggacttctt atgaaggtca atcgatttcg
     1921 gacattaagg aagacgtcaa taattggaag atatatacag aaaatgtaaa atcacagatt
     1981 gttgaaaaaa aagaggcttt aattaagaat ggtttttgga acaaaatacc atggaatttt
     2041 caacaaacta ttaatgaatc aatcttctgt ttaggaacat ttcttagtga ttttcagata
     2101 gttgaatcag cttttgagga aaattttatt tctgagagag agataaaact tctaaggaaa
     2161 attggtaaaa attctgtgca atttaaccat agttacggaa aatactttaa ggaagaatac
     2221 gaatggcgcg aatatggaaa tccagaattt gaaatcgttg aatcaattta ccaacatggg
     2281 agagattttt ttgtgacgtt acaagatgca agtaatgcag caggacgact ggaggactat
     2341 atgagcaaca attcaatata taataaagta aatatatttg gaaatagtta caacactaat
     2401 atacagcaag gccacaataa taatatgatt gtttctattg atgatatgtc ggaattatta
     2461 tcaaagcttg atttgctttt ggagaatttg aatgattcct tacctgaaga agaggtagaa
     2521 gaggctcagg agtatatcga aaccattaaa gatgaagcta ttaaagaaaa gcctaaaaaa
     2581 actatgctga ggtttgcggt agatagtcta aaaaaactta aatggagtac agaattctta
     2641 gcttctgttg caacaatagc tcaacttgtt gctccactgc tataagtgga gaatgaggtg
     2701 ttcttcaatt aaccgacttg ccaagttgtg gtaatggttt gagataatga aagctttgta
     2761 ttctatagag attgatgtat ctgaatgaat gcttgttttt ttggatatag ctgattaatc
     2821 agtggtgtta cattatttat aagtcgtttg attaactagt tcttacgatt tcttatcaaa
     2881 agcacgccac gcaacttgaa tacgtttttg aatacgacta aaaaaccact taaggtgaat
     2941 tagacttaat aaagtgatta gttctattca aaaatcgttg aaataccaac tatcgtcaat
     3001 tatctttaat gagcttaact tcatggcgga agaagaagaa tagactataa agctaggaga
     3061 aaccttgata tgatagggtt tctccttttt ttatttttta ctgtatgcca tattgtatgc
     3121 catctagtca atatttaagt gttgtactaa tttttcaaca gtcttttctt tcgtttcttt
     3181 tgccaagtga ccgtaacaat cgatcgtctg actaattttt gtatgccgta atctagtttg
     3241 aacttctacc atagtagctc cagcgtccaa gagcattgaa gcatgagtgt gtcttgctac
     3301 atgcacaccg agaaatggaa ccatagcttt ttcacaaata gtaatcaatc ttgcataaat
     3361 agcgtggtgt gtgattagtt ctttatagat accacaaaaa atcatagtcg gatcagttac
     3421 accaagtgtc agcatatatt tccgctgatt aatctgccat ttttttaata tagaaattgt
     3481 tctttcatct agtggaagaa ccccagcact acaatcagtt ttaggatcac tgattttata
     3541 gccatttttg gattggctta gtgttttatt gatggtgacc gtattttctt gaaaatcaat
     3601 gtctgaccaa ttcaatgcaa gggcttcact gatcctaacc ccgctataaa agagaaagcg
     3661 tagtaacgtt ttatcaaagt cgtttcgata agtttcaggt ttgctgttta ggtaatcaaa
     3721 caaggtgatc agttgttctt tactgtaaac tttgaccttt tctttatgct tagtggtggt
     3781 tcttttaggc attaatacgt tgtctagtgg attagtctca ataatcccaa gattgatagc
     3841 gtagctaaaa atacgcttag aagttgaatg aagtaccttg tattggtcag tagtttcagc
     3901 ccagctattt acaatttgtt gggctttttt taccgtgact tcattcacac gaagatttcc
     3961 atattttgga agaatccact ttctaaactt gatttcagtt ttcatgaagg tggcttctcg
     4021 gacagtggtt ttatatgttt ctagccacaa gtcataaact tctttaaacg tttcgttgtt
     4081 cgctttttta ataccgtttt tatcgtagtc cacttgcaag cgtgacaagg caagctgggc
     4141 ttcttttttt gttttgtaac ctcgtctacg aaccttatca ggtttgccag tcattttgtt
     4201 cattccaaga taggcttcat agagccaaaa tttacctttt ttagtttcat attgcttgaa
     4261 tgttgccata atagttttcc ctccatagtg tagcaagccg tgggggcggt tggaggaggc
     4321 agtttattat tattttgaat agtgtttatt tagctcagta acaagagctg tgaactcttt
     4381 tagaagatca ttttcataaa attcttgata ctcttttttc tcatctgtat ttttttctga
     4441 atcattaatt aatgagtcat atcgtaaaag cgtatttaga agagctgcga gataagcaat
     4501 tggggtactc ccgtcaggaa attcaattcg agatcctgtt ttaagatatt ttattgcgtt
     4561 tgttaaataa atcttctgtg agacagtcag gttatcttct tcgatgttat tgatagcatt
     4621 agaaagatta gaatcaatta tagagttatt tttttcaata gtttttttca attgcttttc
     4681 aagcacattt cgcttaattt cgggcatcat atgcatatct cttaatgtat aagatccgtt
     4741 taatagatag gtagtggata catctaatac ctttgaaagt tgtttaagcc tggtgttatt
     4801 gggaagatta tagccatttt cccaattact aacggcacct ttactggccg gtggggataa
     4861 tttacctcca agttgttcca ttgagagtcc cttttcaatt ctagccagtc taattctttg
     4921 tcctgtctcc actgcttcgg ggtatttttt tctagccaca ttttcaccac cattcatata
     4981 ttttctctac tatatcacga tttaaatatg tgtgaaacat aaaagttcaa aaaagtatgt
     5041 taaaattctt gcaaatgaaa aggtatgatg gtatattgag tttataagag gtatagaaat
     5101 gtattcaaga gcctcttgca tttttttaaa atctttgtat agaaatgtat gctaacgagg
     5161 ggggataaat atggaattaa aaattgacaa actcacgctt gatgatccag cttttaaaat
     5221 tattgacgat cgagttgttc aacaagtaga gcaaactcaa ggaacgttac tttcattatt
     5281 agaaaaaaag taccagtttc caaaatatat gaacaaggca caagctgcta agtatatgaa
     5341 cgtttcatac aatactatga tgcagaaata tgttcctaac ggtttaaagt tgattatagt
     5401 tgatggagtc gtaagaattt cacaagatga atgtgatcgc tttatggaag aataccaaaa
     5461 ataaacgcaa gccgtggggg caaagggatt aataggagga aataataatg gaatcgacac
     5521 aaaataaagc aatcgaaaaa gtattatcaa caattcttac agaagacaca gcggcagaat
     5581 tagcaaactt ggaaggcaaa gcgctagaag aaacgtttga atgtttgtac gaacaaatgg
     5641 actatcaaaa attgctacca caagaaccaa cggtaagcgg agtattgcgt ggcttgaacg
     5701 atttagtaca agcagagttt aaagaacgac tttctattga agagtatcaa gaaattttgt
     5761 atcagcaagt tgagttgtta gcgagcttgc tagggcttga actggaggac gttgaatgaa
     5821 aactgaaatt tggaacggac acattataag atttgtcgat attaatgatg aatggtgggc
     5881 agtggcgaaa gatgttgcgg aagcgttagg tcttaaacag gttactagag ccattcactc
     5941 tttgcctaaa gatggggtta ctactagtaa ggtcatcgat tcattgggca gaacacaaga
     6001 tgtaaacatc atcaacgaaa aaaaatattt accgcatggc attcaaaagc cgtaaaaaag
     6061 aagccgaggc atttcaagac tggattttcg acatcatcaa agaactacgc cagtccacag
     6121 gacttgaagg ctttcaagta ttccgcatgc tagacaaaga acaccaaaaa gaagcaatga
     6181 ctaaattaag ccacgctatt actgaaccaa aaccagtaga ctacattaaa gcgaatgtga
     6241 tagccaataa agctgtatca actatttatg gtcattccaa aatggtaaaa aagaaagata
     6301 tgactcctga aatgttggtt gatcgtgaac ctattttaga tgagacagta gaattaatga
     6361 ctgttaaaga aaaatatggg ttgcagtttt cagtgagtga gaaaatttat aatcgttctg
     6421 ctgaattgca gacaacttag gagcattaac atgatcgaga acaaagaaaa agccttatcc
     6481 ataacagtct tggcggacag gataaaggca acttaattgg gataaaaccc gatattcttt
     6541 gttccgatta taccatatta aaaaatcgga gggaaccatt atgaaacgaa acttaattat
     6601 aggaagcatt ttaatcggcg gaattttcgg cttattacca ttaattgcaa aaatagctat
     6661 ctttgcagga ttagcgttat atctcttcac tgcttatgac gagtacgact atcaattaag
     6721 aactggaggg cataagtaga tgtacaaaac aaaactatta aaccaactcg atagccttga
     6781 attagaggaa attaatcaag ggatcgctga actagaaaat aatatcggga aaacctactt
     6841 tggaaattca ttcaatgaaa aactaacagt tttgtatgta ctcaaaaaac atgcagaaca
     6901 caaaataatt tgtcgagaaa tcaacgagct aaaaaatcag attttaaccg catggttaaa
     6961 cattactgat atgcaagaag caagggtgaa aacatttaat acatgggtaa agtatcagaa
     7021 tcaattaaaa ggtgctgaat ttgttcggga tgggttgaaa tacgaacttg agcagttgaa
     7081 gctcatggag gtgtctgaat gatttatacc agtattttga aaaaggagga gaggtttatt
     7141 ggcaaataat tatcaagcag caacggaatt acaaaagtcg ggatggtcaa tttatccctt
     7201 atctcctgga actaaaattc caataaaagg ctctagtggt tggaatgatg caactaacga
     7261 ttctaatcaa attaatgaat ggtggacgga agatgcaact agaaatattg ggttaatgct
     7321 tgaagatcat caaatggtgg tagttgacct agatcaacat tctgaaacac gaaacggcgt
     7381 tgagaattat aaaaaacttg cttcaatgta tgatccattt ccttctactt atacggaaat
     7441 cacaccaggt aaaggaatcc atttcttctt taaaattcct gatggtgtaa ttatccaaca
     7501 agaaacaggt gcatttgctg atatcttcgg gcgtgatgat aaaggtaaaa gtttaagcgg
     7561 gattgatatt atcactaaag gagttccaat agcgccaact atgattattt caagaagtgt
     7621 tgggagagca tatgaaccta ttgaaaacgt atctctaaat aatattgcac cagctcccga
     7681 ttggctagtg aacctactgc taaaaaaacc gcaagaaata aagactcaat accgacccgt
     7741 agcaaaaaca gggacagcaa aaaaattaga tatgattgtt caaggcgctg gcgaaggtag
     7801 tagaaacgat catttaacta agttatgcgg ttggttacta tggcacggtg tagataatca
     7861 aactttagca gaattgattt atacagcaaa tgcgtacaat gaaccaccgc taaaagataa
     7921 agaagtgaat caaattattc gatcgatgat taaaaaagat ttgaggggga aagctcatgg
     7981 ctcataagca taaattagat agtattttag actttcccga agccagcgag cgtgaagaca
     8041 atatcattga attgaaaacc tggatgtcac gattaagatg caataaagac gatcagatta
     8101 aaagtaacag tgttgtgaat gcagaattga ttttaaccaa tgatagcaat ttagctggaa
     8161 caattgctta taacgaattt agcggataca ttcacctatt aaaagattct ccttggatca
     8221 atcgtaccgc tggagaatgg gaagacagtt ttgaggatgc cttaacggca tatattgaag
     8281 aaaactacaa tgtagttttt gatgacaaca aaatacacaa agcagtagta aatgtggcta
     8341 gaaagaatgt tttcaaccca gtaaaagaac gtatcgaaaa agttaaatgg gatcaacagc
     8401 ctagactgga aacactgttt attgaccttt taggggtaga cgacaacctt tacacacgag
     8461 aagtaacaaa acgttggata gtcggatcag tcgcacgtat ctataaacca ggaataaagt
     8521 ttgagatcgt tccagttttg gatggaccgc aagggattgg taagagtacc gttccagcat
     8581 tactttacac ggatgatttt ttcaccgatt cattagattc gctaggtgaa aagaaagatg
     8641 attatatgca gcttcaaggc aatgtaatta ttgaacttgg agaattatct tcaatgaaca
     8701 aaactaaaat tgaacagata aaaaacttca tcagtgccaa gatcgataaa attagacctc
     8761 cttatggtcg gaatgtcata gcttgggcaa ggcaatgtgt atttattgga acgtcgaatg
     8821 atgggcagta tttgaaagat gatacaggaa accgccgttt ttatcctttg ccttgtaaga
     8881 ataagccaaa gatgaatccg tttaaaacaa aggatgaata ttttcttcaa gtgttagctg
     8941 aagcaaaggt gctttttgat aaaggacaac gaatctattt tagtcccgat gaagataaag
     9001 aagtcttaga aattgcaaaa gactatcaag aggatgccaa gatggagaat ccaattaaag
     9061 aagcaatcac taaatatttg gaaatggaaa tcccgtataa atgggaagaa gctccagcgt
     9121 gggcgagacg ttcttattat cagaactatc ctgacagtaa atgtaatgag aaaaatttac
     9181 agcatttcta taatggagct actatgcaac aagagtttta tttagttgat agcgtactca
     9241 ccgctgatat tttggaagct gtttttgata aacaagcaaa ggatttattg aatggtcgat
     9301 cagatgcaga aactaaaaag attgccttga ttattagcag tattcctggt tgggaacgga
     9361 agcaactgcc aaaaagaaat aaacgacgag gattttacaa taagtcaaat gcctatgaaa
     9421 ataaaaatcg agctggaaag tacaaaaagt aacgggtagt aacgggtcag taacacctag
     9481 cccgttacta gtttaccctt actgccccaa gggatacagc aatttggtaa cggggtaaca
     9541 ccttatttcc ttaaaacttt tgaaatagtt agctaatatg ctggggaata atgttactgg
     9601 attttacccg ttacctgtta ccaaaacgct agaacccttg tccccgttgg gattgagtgg
     9661 taacgggtac ctgttacgta ggtgttacca cgtgttacga atcaaggagg aaagtaaatg
     9721 ctcaatatta tttcaacaaa caaagcaccg aactttcaat atacagatga aatggatcgg
     9781 tttctaatga acacgctggc atttagtgtg ggattggtaa cagaagatta ttccacattt
     9841 gatccagaag tattaaagat aatggaagaa gaacctgatt ggctgcagga gtcagtagcg
     9901 tggtgtcaat cgctagtagt gggatcattg gtagatagtg gcaactatga tgatacaggt
     9961 gagctaatgg atgaatttaa ttgtctgctt aacctatatg atcgagcgag acaacgagag
    10021 cttacatcga atgaggataa tttgttttta aacatccatg ataagttctt ggctttgcta
    10081 ctaacagatg atgaattgat aactaattta ttggaggtag aataattatg catatggaaa
    10141 gtattagata cttcgatggt gagaaacaca ttgagtattc aaacactcaa gaagatgtag
    10201 actttattag ttttgaggaa gataaaatca ctaaagttaa ttttaaagac ggaacgtgct
    10261 tgaagatagt ttcgccttat attgaataca aatcaaaatg ggaacaagat gattctgacg
    10321 aacgagatcg ctttggttgg taatctatgg cagttaaaaa acaatgtaat catgctggat
    10381 gtaaaacatt gattgattat aggcagaagt attgtgggaa gcataaagca aagcaaacgg
    10441 caattaagcg tgaagaaaga aagcaaagcg aaggtaaata ttttcaattc tatcaaagta
    10501 gaacatggcg aaaggcttca tacttgtatc ggttaaatca tccagtatgt gaggattgcc
    10561 tagaagaagg cttgataagg aaagctgatg ttgtagatca caaaattgaa ctaaaagatg
    10621 attggaatag acggttagat gaaactaatt ttcgttcttt atgccatgca caccataatt
    10681 ccaagacagc caatgaaaga caaagacgag aaaagagtac cctttgagtt aagggggcat
    10741 agtgtgaaac ttactgacaa tcgatgccta ctcattttgg tacaaataac cgttgtgaaa
    10801 aggcataagg gtaattacaa ataacgatta cacttgtaat cacaagtgaa aaagtatata
    10861 atgaaagtag gagatttatt gaaaaataca attgttatta ctgataaaac agggaaacag
    10921 cgcaccattg agttacctca ttttggtcaa gtgaccattc aaatgcagaa cggaaaaatc
    10981 atttatatag ataaattaga taaagaaaaa ttctgatcga aaaacgaagg aatgtgagct
    11041 taattgctta cgttcctttt tcttttgtct gaaaggagga caacatggga caccctaaaa
    11101 tattacacga tacaaaaggc aatatatcaa gtgaagaaaa agcaatccgt gtggacgcta
    11161 gagaagaatt gttcaagcag caaccattaa taaatatcac gccccctgac tggatggcag
    11221 cgagcgctag aagtgaatgg aatcgtatcg taccaacatt aaaaaaagat tatccattga
    11281 gtgaagcaga ttatggttca ttggtagcct attgtttagc ctttgctcga atgaaaacag
    11341 ccgaagccga gataagaaaa tcaggtacgt ttatcacatg cgaaaacgga gtaaagaaag
    11401 ccaatccagc ggttcgggtt caatcacaag ctatgagtga tttgaaaaaa caagccacct
    11461 cactaggtat gaccttagaa tcacgatcaa aactagcttt gaacaaggtt aaaaatgatg
    11521 aacccgaaga cccgttcaaa gagttgatga attcatgaat gattacattg agaaagtcct
    11581 atcaggtaag ctgattgcac ccaagaaaat tatccaagcg tgtgagcgtc atataagcga
    11641 tttggagcgt tctaagtcag atagcttccc ttatgtgttc gatgaagaac aagccaccaa
    11701 agcgattaaa ttcattgagt tgctaccatc tacggacggt aaagcaatca agatgttagg
    11761 atttcaaaaa ttcattctag gcagtcttta tggctggcgt actaaagaag ggaattacag
    11821 gcgattcaat cgagcgttta ccagtatgag ccgtaagaat gggaaaacgt atatcgcaag
    11881 tggcatggct gccaatgcgt tgattatgga acaagaacca gcagaagcaa ggcaagtatt
    11941 gtttgtaagt aacgctttga aacaagctaa attgggctat gatatgctgt ctaattcact
    12001 tagaaacgtg gtcaagtcta gtaagttttt aagaccacaa ctgaaaatta tgaactctaa
    12061 gattcagcac ttgccctcta attcgttcgc tatggcactg gctagtgaaa ccagcacgct
    12121 agatgggttt gcaccaacaa ccgcaattct tgacgagtgg cacgaagcaa aaactcgtaa
    12181 aacgtacaac gtcattaagt caggaatgac ccaacaaaag aacggcttat tgtgtgttat
    12241 tagtaccgct gggcttgatt taaacgttcc tatgtacgag gaatacttat tgttagatcg
    12301 tgtgctaaaa ggcgaagaac aagccgacag gtactttata gcgatatggg aattggacga
    12361 tcccgaagaa attcacgatc aagagaaatg gatcaaagcc aatccgattt ttgaaagtga
    12421 agaaatcaaa aaagtaatga ttccaaccat tcaagatgat gtgaaccttg ctttgaaaca
    12481 aaataacctt aattctgtat tggtgaaaaa cttcaattta tggagacaag cgagtgagga
    12541 cagctatatg attgctgaag actggcaggc aaccgaagta gaaccacaag atattacagg
    12601 caagcccgtt tatatcggag tggatttatc taaaacagat gatttaacta gcgtttcatg
    12661 gatcgtaccg ctagataacg gcgaacttta ttgtgattca catagctttg tagccaccaa
    12721 atatgggctt caagacaaag aaaagcgcga cggtttgcct tatcgagaac tagaaaaagc
    12781 tggtgagtgt tccattactc aattagaaag tggaatcgtg gactatgacc aagtatttca
    12841 gtttattcaa gatttgattc aagaaaatga tttggaatgt atggggattt gttatgaccc
    12901 gtataacgct aattcgctta tcagtaaagc tgaaaaagcc aactacccaa tgttagaagt
    12961 aagacaagga acgattactc taaacgttcc gacccgaact tttagagaac aagtttatga
    13021 aggcaacgtt attcacaata aaaatacgat tctcacccat gcagtgaaca acgctatttt
    13081 aaaaacggat aacaacggca ttcagattaa taaatcaaag aacagtaaca aaattgatcc
    13141 aatagctgca ttaatcaatg cctatgtgtt tgcaatggat tacttcacca caacggaagg
    13201 agcgaaagca gacaatgaat tttatacaag tgaagaattt tctttctaat tacattcata
    13261 ccgttctttt acttcttgga ttggtgtgtg tgttggttgc tatcacctta ctaacaaatg
    13321 tctactatgg cttgttagcg ctgggcatag tgcttattgg ggtagcggtc atgctaaata
    13381 cagaacagaa gggaggttaa aagatggcat tttttaaagc gagacaaaat acaacgggag
    13441 atcctttcct acaacatgtg gtatcaatcc aatcggatga ttacaccacc agctttacaa
    13501 gcgttcgtgc attaagaaat agtgatgtgt ttgcagccgt tcgaattatt gctagtgata
    13561 ttgcttcaag tccgattcaa ttggtcaaaa acaatatgcc acaagctgat aatgaactgg
    13621 tgaagttact aaacgagaaa cctaattcag aaatggatgg ttggcattta aagtttgctt
    13681 tggcggtcaa tatgttgcta aacggtaata gctttgcaga gattaaacgc aacggtgaaa
    13741 aggtagaaga aattcactta ttacctaact caagtgtaac ggttactcaa ttagataatg
    13801 gtacgttgtc ttatcagatt ggtgataaaa aaagacgtgt gaagtctagc gatattttgc
    13861 acttcaaata tttcacccaa gatggtttaa caggattacc accactttat gcgttacgtg
    13921 atgaactgaa aattcaacaa gctggcaacc gcacattgca caatttcttt actcgtggtg
    13981 tcagtgggtc aggtattttg aaggttcata agtcagattt agacggatca gcaaagaacg
    14041 caattcgtga aaagtttgaa gaagccaacg gttcaagtag tggagataat gctcttagaa
    14101 cgatcattct tgatgaaaca atggactaca aaacattaga aataaacacc gatgttttga
    14161 agcttatcaa ttccaatgat tggaacacaa aacaaattgc caaggcgttt ggtgtaccga
    14221 ttgagcgttt aggcgttgaa aatgaacatt ctagcaccgt tcaaagtaat ctccagtata
    14281 tccaaagtac gctgattcat tactttaatg tgtttgtgag tgaattagat acgaaacttg
    14341 aaaccaatat ccgctttaat tccgatcagt tactagagac tgatcctgaa acaaaagtaa
    14401 aaaatgtatt ggatcaggtc aaagggtcac ttctcacgat taatgagggg cggtcgaaaa
    14461 tggggctacc ccccatggat ggtggcgatc gtttactagc aagtttgaac tttacgtatt
    14521 tagatacgtt ggagaaatat caattaaaag aacaggaagg agttacacca gttgaataat
    14581 gaagaagaaa aggaaaaacg gctgacagaa gaagctgagc taacagccga ttctcccaaa
    14641 gtagagaaag aaaatgaaaa acaaccaaca gacggcaaaa ctatttcagg ctatgcgttg
    14701 aaattcgggc aaccgtcaaa agatttaggc ggctttgtag aagtgattac acccgaagca
    14761 ttaaaagagg tggatttatc aaatgtgttc ttattatatg gacacgatta ttcaaagcca
    14821 ctagctagtg tcaaagcagg tacgttgaaa ttgaatgtcg atgatacagg attacatttt
    14881 gaagcagagc taaccgacac gacctattca aatgatgtgt atgagaatat ctcaaaaggg
    14941 gttgttgatt ctatgtcctt tgggttcgtg cttggattag attcattcga caaaaaagaa
    15001 gatggcacaa ttgaacgatc aatagataaa atcaaagcac ttaatgaaat tagtgtcgtg
    15061 accgttcccg cttatgattc atcaaatgtc caagtaaata agcgttctta cgaatcgttt
    15121 atgagtaaca accaagcaaa acaaacaaac aatagcttag aatccacttc taaagcacaa
    15181 aaggagagta ataacatgga aaaaacttta atcgataacg aaaaaacaga attgcgtggg
    15241 tatgaagaat atatccgttc acaaggcgaa gtgcgtgatg gagtcactac tgtaaatgca
    15301 gcggcagttg ttcccgaaga agtaatcggt gaagtctttg atttgaaacg ttcaaattat
    15361 aacttagctc aatatgcaac agtaaaaaca gtatcaaatg gacaaggtaa atatccagta
    15421 gcaactaacc aacaagcagt gttagcaaca aaagctgaac ttgctgaaat tggtgatatt
    15481 gacgctgaaa tgtttacttc agttgattat aaagtagaaa ctcgtgctgg taagattgcc
    15541 ttatcaaatg aggttgtgga agattcagca gtgaatattg tacaagaggt caaagatcag
    15601 ttagcgaaat tggtagaaaa cactgacaat aagcatatca tggatttatt aaaaacattc
    15661 actaagaaaa cggctgctac gttggacgat ttgaaacaac tatacaatgt ggcattagac
    15721 ccagcattaa ataaaatggt aattctaaac caaagcggat attaccacct agatacattg
    15781 aaagattcag atggacgtta cattttacaa cccgatgtga cagcacctag tggtaaatca
    15841 ttattcggta tgccagtagt attgattgca gatacattgt ttgccaatcc taaagctgga
    15901 acattcccta tgattatggg ggatattgca caatctatct ttgttgctcg tagaaatcaa
    15961 gtaacgactc aatgggaaaa attcgattac tactcacaag gacttgcagt gatcgttcgc
    16021 aacgattata agaaaattga tcaaaatgct tcagtgtata ttgagtttac gccagttgta
    16081 acaccaaaag tatagaaaaa tattgggcgg cggtttatcc cactgccttt ttttcattag
    16141 gagagataaa atggtaattt tggatagcat aaaaaaaagt atgcggatcg atcacactat
    16201 tgatgatgac tttattcaac aattgattga tacagcaggc gaatatataa aaagtgctat
    16261 tgatagtagc gcaacggata aagatatgga taattatcag caatttgatt tggcggtgtc
    16321 attacttact caacattggt atttgaaccg tcaagaagcc agcagcgaac ggataccagt
    16381 aacggtacaa gcattagtac aacaaatgag aggtgcttat tatgccaatc attaagaatg
    16441 taaatgaatt gactgagaga gtcaagttta aaaagacaaa acgggtaaaa gatgaagatg
    16501 ggcagatggt ggatagtgaa gaaactgtat ttgaatgttg ggctaatgtg cgttcacaaa
    16561 tgctaaaaga cgtgcttgct agtgtaggta ccattcttga aggaacgttg acgtttatta
    16621 ttcgatacga tcaagattat gaactaacaa acgatatgaa agtagcttgg aaaaacaaga
    16681 gttataaaat tatttcaatc aatgaaggaa cggcgtttaa agattataca acgatcatag
    16741 ctaaacagat ttcttagaat gattacactt gtaaacgttg tgggtattta gtataattaa
    16801 ggtagtaaat gaagggttag ctgcctgaat tttgcaagac ttagctagtc gaaatctatt
    16861 gttggactga aaattgtagg tgtggttgca aacatgctgg actaagaaag atgatagatg
    16921 atgaacgttc aattttaata caggcatgct aggactgatt ggatggtttt ggcaagctga
    16981 ttgtgtagaa atcaaagaac acgtctgttg tggacgtgtt ttttttgtta taattcaaaa
    17041 gaggtgattt aatggacaaa gcagagttta caaattttta ttggaagtat tatctacatt
    17101 tagaaaatga gtttattaat accacagatt ttgtgatgct cgatgagaat aatttcaaaa
    17161 cattctctat tgaatatcaa aaactactac tagcgattgg ttcggagtgt gagattattt
    17221 tcaaagaact ttgcggattt gatagcagta gcaataaaag cataactgat tttaaagctg
    17281 ttattaaatc tagcgaatta atgtgtttgg ataatggtgt tagggttatg aattctttaa
    17341 cattgacttc actaaaacct tttggagatg ggtggcctga agatacacca gattggtgga
    17401 aaatatataa cagagtaaaa catggtcgtt cttcaaatta taaggaagca aatttggaaa
    17461 atgtgttgta tgctttagca agtctatatt tattagagga atatttgcat aagaaagtaa
    17521 tactagaagg tgaagtagac tttataagtc cagagtcaaa tctatttacg ttatcttggg
    17581 aaagaaaaca ttcgtcgatg caaaaagtga cgtttgaaaa agtggattca aattatgtag
    17641 ctccactcat tgattttaaa gaacaagaag aaatttaaaa acccccacgg cttattgaag
    17701 tttttgtatg ccatattgta tgccatttgg tttaaatcgc ttgaaaaata gtgatagata
    17761 actctaattc atttagtcat aaacgctgtt ttgataacct ttatagttct ttgatatggg
    17821 tttctcattc atggcggaag aagaagaata gaacaaaaga atgaattaat aagatataaa
    17881 aaaagggatt gtgacaaacg tcttgtcaca atcccttttt ccaaataaac gatgttcaat
    17941 cagctgcccc tcggcattcg ctcgaaaaac ggcgaaatat gaagaactat tttgccattt
    18001 gtttctctaa taatcaggtc aaaacgttga cttcacagtc tcttccttaa acggtgttct
    18061 cctgccaact cctcggtcac aagtcacaat attcaacaaa catgagaagc tgttttttga
    18121 aattgttcct tgtgactcgg agctgaacgg caggctcacg acctctttct taaacggtgt
    18181 tcaaaagctt gcatctgcgg taataagttc aaccaaacga aaattatgag gagctatttt
    18241 tgtttggctg ttcttattac ttgatgcagg acagcttttt cacaacctct tttcgtttag
    18301 cactaaattt ccttaaaaag tagaaaagct ttcgaagcct cgtttgtttc ttttactgta
    18361 taataaaaga aaagagtaac aatcgctaaa agatataaac aagagaaagg aaatacaaga
    18421 tgattggtct gatttatttt gtggtgatcg ttctggcgaa cacagttggc gctatttccg
    18481 ggatgggtgg aggcgtgttg atcaaaccga ttcttgatct tatcggcgcc cattctgttg
    18541 cagggatttc tttttactca acagttgcgg tattcacgat gtccattgtt tcgactgtgc
    18601 gacaagtatc aagcgggaaa tcactgaatt ggcagattgt cggttgggta tctagtggag
    18661 ctgttgttgg aggagtagtc ggcaacattg tattcgaagt ttttttacag ttatttgaaa
    18721 acgagaagca tgtgcagctg atccaaattt ttttgacggt attgaccttg gtttttgcgt
    18781 tcttctacac caagcatcat cagccaaaat ttcatttgac gtcatggact tggtacctta
    18841 tatgtggggg ggttctgggc tttttagcta gttttttagg tattggtggt ggaccagtca
    18901 atgtttcttt gttgatgctg atgtttgcat tgccaataaa agaagcaaca ctatattctc
    18961 tatctacgat tttcttttct caactggcaa aattggtaac gattgcttta acaagtagct
    19021 ttatgcgctt tgatttatct atgttgtttt atgttatccc cgctgctgtt attggaggtt
    19081 tgtggggagc aagatttagc agggttttgt ctccaaaaaa agtgaccttt atttttcaag
    19141 taattgtcct cgtggttctt ttggtcaatt gttacaatgc ctttgtcatc ttacaaacat
    19201 taggagaatc tagactaacg caagtcaaaa tcactgaaac atattgttct aaatgaaaaa
    19261 aagagtatac tatgataaaa aaattcattt taaaaaatca gaaattggct ggtgatttgg
    19321 atgttacggg aaatgcgttt agaagatgca gaagcactac gtgaaatcaa tgcggatcaa
    19381 ttagggtatg atataccatt aaccgttacg gtgagacaaa tgaagaaact gctgaaggag
    19441 ccagagaaaa attttttctt ggtttatgaa gatcctactt ctaaaaaagc agctggctat
    19501 gtacaagcgg agtcctatga atcgattttt agcgatccaa tgttcaatat tatggcactt
    19561 gctgtgtcta aatatgctga aaaaagaggc atcggaaagg ctttgatgac tggcgtagaa
    19621 gaagaagcga aaaaacgagg aataacgaca ataaggctta attctgcaga atacagagta
    19681 gaagcacacc aattttatga acacatcggt tatcattccg ataaaatgca aaaaagattt
    19741 ttgaaaatcc tgtaaatctt tatcaaaaaa atacccgatc accaagccga tacttgatga
    19801 tcaggtattt ttgttttttt gactaagaaa gtaattgcat caagaaacca gagatgatca
    19861 aaacgatggc accaccaagt cgattgccca tttgagcaaa agcaataagt tccatccgat
    19921 tagctgcaga taagacggct acatttcctg ttccgcccat cgagttgttg cataagccag
    19981 cagtaatagt tgattcaatt ggatataaac caaacaaacg accgacaaat cctgaaacaa
    20041 ctgaaatcgc gataacactt gtcagacata agacaacaaa ttgccatgtc aaagcagaag
    20101 ctaatacatt cagattcagc aacgcaatac cgataccggc aagaacagct gctgtcaagt
    20161 ttttgacaat caagttattg aacatgatag ctgcatcttc ataatactct ggtaataggt
    20221 tggcgatttt acagatgaca acgataataa tcataaatgc gtaagcatgg actttaggaa
    20281 caaaatagtt accaatttgt cctaagatga agaaagtcaa tgataccatc aaaccaacac
    20341 ctaactgtgg aatggaaggt ttcacagttc gaggttttcc atcgccaaga tcgccttttt
    20401 ccattaattt accattgccg ttgaattttg gcattgattg tccagcacga gcaactaatg
    20461 ctgcaccgat gatagctaag acatttgtca ttgctgtcgc aggaatcagg cgggaaatga
    20521 tagaagaagc atccgtacct aaaacatttg cataaatcgt tgataaagga acggctccgg
    20581 ctccaacacc accagccatc gcaggtaatg agatgtataa aacggaattt gcaaaaccat
    20641 ttccgataag catgcccacg agtcctacgg caaaaaagga gacgaccatt gaaataagtg
    20701 caacgggaat aaaacgaaca gaagctttca tcaatagatt acggttcata ccaaggatgc
    20761 taccagtaat caaggcagca atataaaagt cccaaaagcc catattgctg acaaagtttt
    20821 ctgtggattt cactacatat tcaggtaaga tcccaaatgt agcaatcgca gcagaagcga
    20881 agatcgcaaa aacggaacca ccacccaaat aacttttgac gatagggatt tttgtcccga
    20941 taaaatgaaa taaattaccg aaaataacca aaactgcgat tggtccaatc atattcgtcg
    21001 gtaatttccc taaagccatt gcaatgatca ggacaaggac catgatcaaa taaagtggca
    21061 aacttacgcc ggtgattttt gtggaccaaa aaccttcttt tttcttttcc tcaactgcag
    21121 ttgcagttgt tgttttttgt gccattttta cagatccttt cttattctgt atcatatgaa
    21181 aaaagccaaa gaaaagcgac aaaagagcag gagttggctg gccgctgttt tgccgcagtc
    21241 gcttttttct ttttagacgt catacaaagt ttctgtatgt tcttttacta taacgatctt
    21301 aatcttctaa agatttatat tcaggaaccc atttagcaga ttcaactgct tctttgatat
    21361 ccgtgatttc ttcacgattc aagttttgtg cgacaactga ttttgcgaca gtttcagcaa
    21421 tcgtttgaga aaattcagtg atttttgcta ctggaggcaa gatagctgct cctggttttg
    21481 ttacatcaac gattccgcct aatgctcgac tagcttgaga aatgatttca gcatttactc
    21541 gtgtagctgt tgaagcaatc aaaccaagtc ctaaacctgg gtacatcaaa gcattatttg
    21601 cttgaccgat ttggtaagtt acaccattat attcaacatc agcagcaggg atacctgttc
    21661 caactaatgc ttttccgtcc gtccattcta ttaaatcttt tgcttttgct tctgccaatt
    21721 ttgttggatt agacaaaggc atgatgatcg gacgtggtgt atgagcagcc atttctttca
    21781 cgatttcttc agtgaatgct ccaggttggg tagaagtacc aatcatgatt gtcggatgga
    21841 tcgcttttac aactgcttct aagtttgtca attcatcagc attagtgaaa tctgagcgtt
    21901 tgcgagcgaa tgggatttga ccaggagtca atccttctgt atcttcaaac aataaacctt
    21961 gtttatctac ttgatagaaa tgttggcgtg cttcttcttc aggtacacct tgacggatca
    22021 tctcatctaa aaggatgttt gcgataccca caccagcagt accagcacca aatgtcaaaa
    22081 ttatttgatc tttcaattgt tcaccggata tattcaatcc gcctaataca cctgctagga
    22141 caacaattcc tgtaccttgg atatcgtcgt tgaaagtagt gatttgatct tcgtattttt
    22201 ctaagatcgc tgcagcattt cctcgtccaa aatcttccca atgcaataac aattctggga
    22261 ataattcggt agctgattga acgaattgat cgatgaattc atagtaagtt tcgccttcta
    22321 cacgagcatg acgattgcct aagtataagg gattattcaa taattcttca ttattagtac
    22381 cagcgtcgat cgatacaggc aaaacttgtg ctggattaat accagcagca gcagtataga
    22441 ccatcaattt gccgatagcg atgtcaacac cgttcacacc ccagtcgccc atacctaaga
    22501 ttccttctgc atcagtcaca acaatcaaac gaatgtctcg tccatctgcc gcgttcttca
    22561 aacttgcttt gatgttttct ggttcgtcga ttgaaagaaa tgcagcatct tgcggtttca
    22621 agaaaatttc gttgtattgt tcgattgaat ccgcaacgac aggatcataa acgacaggca
    22681 tgaattcaac taaatgttcg cccatcaatt tataaaacaa tgtccgattc gtattgaata
    22741 gattcattaa gaagatacgt ttttccaaat ctgtttgttt gcttaagtat tgaccataag
    22801 cttgaacaga ttgttgttcc agtgtttgga cagtacttgg aagcatacca gtgattccat
    22861 attttgcccg ttcttctttt gtaaaagctg ttcctttatt tagaaaagga ttatttaata
    22921 agtttaaccc tgtaagcatg aatgattcct cctaagtttt aatacaaacg aagtatagaa
    22981 gtcagatgat gatatagtca aaaagagcag gtatgataaa ttttatttat atgtaatata
    23041 gccaaaagaa atcaaaatag cagtatttgt tctttttata tcgacaatcc ttgcttgaat
    23101 actatgcgta tctcttatat gctagagaaa gaaaagtaga agaataaggg atagatggaa
    23161 tggataaata ggatttctga ggaaaaaagc acatgatagc gttgatagga gtcacctatg
    23221 tcaggtgata aaaatacata agtagaaaat taagaggagg aagagaaaat gaaagaagtt
    23281 caattgatta gaaaaagcga actatcagaa ggcggttgca atgcatgtgg cgtagtagaa
    23341 gcgacaagct atacattaaa attagattcc aatcaagcaa taatctctga actgacagta
    23401 ggcggtcttg tggacagtct ggcgcttgct gaaggtttta ctggagaaga tatttatgag
    23461 atgttttcag aagtccgtca gttaaaaaaa ggagaaaaat gcatagaggt tcatcatgag
    23521 agcccgaatg tgaggttcaa acgtggtgat aatgagatga tcttcaagaa tcatgtgtct
    23581 gatcacactg aactatatga aatcgtgaat caaatactgt ctgggatttt tgaattagga
    23641 ccttatgagt tcaaggaaga aaacggtaat ccaaaattaa atgaagaatg gcaagaaacg
    23701 attgaaacac aaagaaacaa tccacactta tttcaatagt cttccacagt atttcgtaaa
    23761 aatgttgatg aaaaagaaac accgtgaatc agaggtcgtg aaaaacctgc cctgcatcaa
    23821 gtaataagaa cagccaaaca aaaatagctc ctcctatttt tcgtttggtt gggcttatta
    23881 ccgcagatgc aagtttttga acaccgttta aggaagagac tgtgaagtca acgttctgac
    23941 ctgagtatta gagagacaaa tggcaaaata gttcctcata ttttgccatt ttttgagcta
    24001 ataccgaggg tcagctgatt gaacaccgtt ttatcgagag gttgtgaaaa agtcgcttag
    24061 tttcgagtag taagaaaaat tttgtgaaaa tggctttcca catttttgca taaatttgac
    24121 ttaataccga gaaaatggct tttgaacacc gtttattcaa aaaaagggac tttgacacta
    24181 cttttgtcaa agtcccatcg gttttttatc tatttttatt acaatcattt cttccagagt
    24241 agtgacatct ggaatggctg aaatgtaaac agattatttg cctgtatcaa tgtcgaagag
    24301 taatataaaa agtgtaaacg ctatcatatt gttcatcatt caaattttat agagagaagg
    24361 cattgagatg gaaaaaacat atcaagcagg tacaaatgaa ggaatagtag attttatcaa
    24421 tatggaagat ttagaaatcg cggcttcaca agtgattcca gctggtggtt atggttacat
    24481 cagcagtggt gctggcgact tatttactta tcaagaaaat gaacgggcat tcaatcatcg
    24541 tttgattatt cctcatgtat taagagatgt agaattaccc gatacaacta cacattttga
    24601 cgaagaaatg ctgactgcac caatcatcat ggcaccagtt gctgctcatg gattagctca
    24661 tgtgaaggcg gagaaagctt ctgcaaaagg tgtagcagat tttgggacga tctatacagc
    24721 aagttcttac gcttcttgta ctttagaaga gataagagaa gcaggcgggg agaaagcacc
    24781 gcaatggttt caattttata tgagcaaaga taatggaatc aatttggata ttcttgaagt
    24841 ggccaagcgt aatggagcaa aagcaatcgt attgacagcg gatgcaacag ttggcggaaa
    24901 ccgtgaaaca gaccgcagaa acgggtttac cttcccgctg ccaatgccga tcgttcaggc
    24961 ttatcaatca ggagtaggcc aaacgatgga tgctgtatat aaatcttcca aacaaaaact
    25021 tagtccaaaa gatgtagaat ttatcgctgc tcattctgat ttgccagtct acgtgaaagg
    25081 tgttcaatca gaagaagatg tctatcgatc attagaatct ggtgctggtg gaatttgggt
    25141 atctaaccat ggtggccgtc aattagatgg ggggcctgct gcgtttgatt cgttgcaata
    25201 tgtggcagaa gcagtagata aacgtgtacc aatcgtgttt gacagcggtg tacgacgtgg
    25261 acaacatgtt ttcaaagcaa tcgcatcagg tgccgatctt gttgctattg gcagacccgt
    25321 tatttatgga ttgtctttag gaggaagtac aggagtccat caagtgtttg atttctttaa
    25381 aacagaattg gaaatggtca tgcaattagc tggaacacaa acagtagaag atatcaaaaa
    25441 aataaaattg cgtgagaatc gttttattta agaaataaat gacgaaaaaa ataggagtgc
    25501 ggcagatgat gccgtactcc tatttttata gttcaaggat gtcactaata gaataatttg
    25561 aaacgaaaaa ataaaattga aatatttttt catacttaat aaaaaaggaa gaatattcat
    25621 gtaaacgatt ttattgtttt tttctctttt atacaaaaag ggtcataatt tcaagtaagc
    25681 ggatgaattc atgaggattt tttgcatact gtctctacta tacttaggtc actaagaaaa
    25741 aggagtgatt tattatgaaa aagaacatga ttgggacatt attactttca acacttcttc
    25801 taggcggctt ggcaacacca gcttttgctg agggtcaagc gacttcaaaa ggagatatta
    25861 cctttactga accaacaaat accgttgagc ctcttaaccc tactgatcct tcaaaaccag
    25921 tagagccagc agatccggaa aatccagcaa ctggccagac aggttcttta acattggatg
    25981 tagttcctga attgccattt ggtacacacg agattgaaag cggaacaaaa acgtatcaag
    26041 tagacgcttc aaaaaacgat acaccttatc tccaagtatc agatcgtcga ggtgtaggtg
    26101 cagatggcca agcacaaggg tggaacgtga cagtttcggt ttcagatttt gtaaacggtt
    26161 cacaagtttt gcaaggcgct gaattagatt ttggaacatc cactgttaaa agtacgtcag
    26221 ataacgaatc cactggacca actagtcaaa ctgtaactgg gttgagtaaa gcaagtgcag
    26281 cgacaccaat atttacagca gcaaaagatc aaggtctggg aacatggctt tctgtttatg
    26341 atccagcaaa tatcacactg aaagtgccga aagcagcagc tggaacattc acggctgatt
    26401 tgacttggaa cctcgtggct ggcccagtag catagagtat gaaataaatt caacaaactg
    26461 cggtgaaaat actataggtg aaggattttt taccgcagtt gttgttgaaa ggatgtggtg
    26521 gacaatgaat caaagtaaac gactgtttgt ttcgtttttt tctattttaa gtctattttt
    26581 catttttccg tcaatcagcc aagcaaagga ttcagcagga gactttggaa tcaaaccggt
    26641 atttccagag aaccaaatag ataaagcaat cggttatttt gatttattag tggcaccgga
    26701 acaaaatcag acactagaag taattatcag caattcttca gatgaagaac gaacatttga
    26761 ggtctctgtc aatccggcgg taacgagtga tggtggaacg atcgattata gtcaaaaaaa
    26821 cccaacgtta gatgagacat tgccgtttga tgtccgtgac gttttgctca ttgcgaaaaa
    26881 ggaaatcaat gtatcagcac atgcagaaac aactatacct atagaagtga aaatcccagc
    26941 aaaaagcttt aagggacgtg tattagcggg tattcatgtt tcacctaaag aagaagctga
    27001 aacagaaaat gctacagaag gagcacaaat caaaaatcgt attgcttata atctcgcagt
    27061 cgttttacaa gaaagtcaag agacgataga accggatctg aaacttttat caggagattt
    27121 ggacgaggtc aacgcaaagc caactgttca gcttcgtttc caaaatcctc agcctaggat
    27181 tatcagcaat ttgatattta cgagcaagat cttttatgaa aatcagcttt atattgaaaa
    27241 tacgtctaat gaatttttag ttgcaccaaa ttcgaatttt cacttgaatc ttgatttagc
    27301 tggtgataaa gcaaaagccg gcgattaccg cgcagaaatc atagcaaaat caggcgacag
    27361 taatgaatgg cgatttacac aaaactttac gatcaaaaaa gaaaaagcac aaaaagtaaa
    27421 tgaaaattcc gtttttgcag tacaagaaca atctttccca tggctttatg caggattggt
    27481 tgctgcagtt ctagtgttat ggttgatttt gctgtttttg tgggtccaca agaaaaagaa
    27541 aaaaggagaa acaacatgat gaggtgattt cgttgaaaac atcgatctta ttgggaggga
    27601 ttattgcaag tttgctgttc ttcagctggc aagagccagt ccaagcagat accaaaagtt
    27661 caggtacgat tacattttat gatctaagtc cagttccgca tccaagtggt tcagtcaatg
    27721 gcgcatcaaa acagcagaca cagctaccga tttataaaga atctaatcaa catggcgtat
    27781 tcccggagac gaatgaagaa aagatccgtt ttctttattt gatcattggt atagaattat
    27841 ggcttcttgc gtggttgttg ttatggagaa gaaaggaaaa ggaggatgaa catgttgcta
    27901 caaaataaat tttcattttt tcgctccttc ttttttactt cgctttttgt tttaagtata
    27961 tttatctttt caccttcggc agaagctgtt ggagcaacgc ctcctgccat ttcttatgat
    28021 ccatacacgt atgaaaatat cgtacctcca aatggtggac gtagtgcagt caatggcggg
    28081 aagcttgtcc aactaactgg ggctaaccaa ttgcctaaat tgactgctac ttatttgaac
    28141 gatggttcaa atgatccttg gaaaggtggc acggtccgtt ttcattggga aagccatgtg
    28201 aaactaacaa atgtacttga tttggtgaat atcactgcac aaggatttgc atttgtggcg
    28261 atcatcaatt tgccggaaca tgtatcaagc agtgatgtat taaaagcaat tgactggaac
    28321 aacgcctact tgtatattgc cggtgccccc atcaaggtta ctagcggtag tttattatca
    28381 gcaggaagcc ataccttgcg ttttgggatg gggtcgtggg atacaaatac tctattggca
    28441 gatattattg ctattattac gcgaggcgaa ttcaacgtgg acgatatccc gcttcaattt
    28501 gatgttacag ctaatgtcgc gaagatgaca gaaaacggag ataaatatga tacgagtcca
    28561 aacaaaattt tgaccaaggg taaattacaa ccttctatgt ctaaaaaggc cgttttttct
    28621 gttgattttt atgattcgaa taatattatc caagatcgta tgttcaattc gattttagct
    28681 ccagctgcag gtagatttta tcctaaatta actggcggaa acttgaatcc aggagcatat
    28741 gcattttctg caagtgcaga gataaatacg tggaatagtt acatctcaac aagtgattca
    28801 acaggaaaat ataatacatt tgctgatacc acagaatacg tggatggaaa taaccagaac
    28861 cttccaggct ctaccaatag tcggacattg acgatggggc tgactgaagg acgttttgat
    28921 cagttgccat ttagtcgctt tgatcgcgta gtcaactatt ttacaggaaa aaacgttaca
    28981 caaggagcaa tactgactca tactccagca agcacaagtg gattgaatca gacagccaat
    29041 atcgtgtaca gtgggaaaga tgcgcaaggc ggattgcttt ctcctgttgc tttaaaggta
    29101 ttggagcagt acaagctaga cggaacggtt ttaccaacaa acttaacagc cgatagtcag
    29161 tggggaaagc tcctttcttc tgcatcgtac caagtcacgg ccaattggtc agcaactgaa
    29221 ctaaaaaagg gttcgatcca ttacaggctt tattcgaaag gaacccaaaa attagcgaca
    29281 ggatggacag atcaactttt ccaaacgatc caatcaaata acgggggtgt aaatacagct
    29341 acttctacac tacctgcatt ggcttcaggg caatattatt ttgattatcg cttggtggac
    29401 gatgtactta ctaacatgta tccgtctttt gcgtacaaat ggcagtctga acaagctgga
    29461 atcaccactc ttcctcaaat aacagtggct aattttccat ctgtgagtgc agatagtcag
    29521 ctaaaaaacc ttagcagaca acctgagaca ggagatcccc tgtctgctct atcaggagat
    29581 acgattcaag aaaatgtgac attcacgtta acaaaatctg gggatgcgat aaccgacaaa
    29641 aaaatcacga tctcacttcc tgaaaatact acttatatca atggttcctt aaaattaaat
    29701 ggtacaacta tcgcagatac cggcattcaa caaggagttt ctgtcccagc agatctattg
    29761 agcaaaatag gagataccat tcatcttact tacaactatc aattaaatac tgtagatact
    29821 tctgtacaat ccgtcagcat actgacaaag gcagctgtac tttccagcaa tattacatta
    29881 gcagatggtg ctaagcttcc taacccagtt gtccaaacat ctgctaaaac aattcttgtc
    29941 cccaaacaag aattaacatt agttaatgta ccagatgatt ttacatttgg taacgatctc
    30001 cccaaaccgt tgaagacaag ttattatgaa gcaaaaggcg atttttcttt tgatgtgcgg
    30061 gatactcggc ttccaagcac gtctccttgg caactgacag ggacattgac ttctttattt
    30121 aagaatgatc aaggtcaaga attaagcgga acaaaattat acttcaatca ttctggatca
    30181 aaacaattga ttcagcaagg tcaaaacacg ttgatttatg agagtgatgg aacagctaaa
    30241 ggagaggttc tcgttgactt tcccgatacc gatggtttac tcttagaggt aaatagcagt
    30301 acgaatgctc aacctggcgc aacgtatcaa ggcatggtca catgggaact gacagctggg
    30361 ccaaccagct aagtgattag aatttgtatg gatctgtgat aactgtcaca gatcctcttt
    30421 ttcaaggatg agaaggaaac ttctgctgtt aaaatcagaa atgcttggag gagtggactt
    30481 ggaacttttc agtttgcttg aaaaaaatga acaacggcaa atcaaacttt tccaactttt
    30541 attagaggaa aaaggagaga tttccgttaa taacgtactt acccgcttat cagttgatcg
    30601 ggctaccttg aaagaagatc tgttttattt acaagctaac ttggaaaata tcaaacaatt
    30661 ggaaattgtt atccaagaag ggaatatttt attaatcaga catggaaata tagcagcagt
    30721 cgatgtttat tatcattgct acttgcgcta ttctacaaaa tatcagatac ttctttatct
    30781 gttggaaaat ggctcatttg atcggcagaa acttgtcatg gaacttaata tcagttcggc
    30841 tactttgtct cgcagagtga aggaattaaa tgattcttta aatgagtttc atctgaaaat
    30901 aaaaagcggg cggctgaccg gtccagaaag tcaaatttgt tatttttatt tccaattatt
    30961 ttggtttgga cgtccttatc ttgaaaacaa aaaagaatac agtgatttat caggaaacac
    31021 ttttttagat ctgctgcaaa aagagtttag ttttactatt acagaagaag gacgattgaa
    31081 atttgaaata tggctttcca ttatgaagaa gcgaatgcgc tgtacgaaaa aatccaaacc
    31141 agattatagc aatcttcctg ttggattgtt tcaaacgaca caatttttat tggacctcca
    31201 tacatttttg acccgttact tctttcaata tgcctttcct tggaatcaaa atgaaacgtt
    31261 ggttttttat cttttcatga tcagtaactt tacattagat acacataact cgcatgtcca
    31321 ggagtttttg ataagcgaaa aatacaaaga cgacgtagta gctcaattga atcgaacgtt
    31381 taaacaatcg atagaagaat atttctcttt ttatgttttt ccagaagaga tttcgcaaaa
    31441 gattgcgttg acagtgatgc aaaatcacgc tcattttgcc tattttagag gatggatgaa
    31501 tatttttggc caagaaggat taaaacagcg gttaactgaa gtagaagata aacaattcct
    31561 ggttttatgt gaaagattgg tcagacaaac atttgctatt gttgatttta ctgaagtaga
    31621 cgaggaaaat gcccgctttg aattatttgc tcgctatgca tctattttgc ggacgctgat
    31681 caagcagtta gaattacgtc tgcaaattgc ttatgatctt ccttatgaaa ggctaatggc
    31741 ggatatcgtg aaagaaatca tccaggaaca attgctgaat acgatcaaat atgatttagt
    31801 cgaatacgag aaagaaaagc agtatgatat cattttgact agccaattga aagagtaccc
    31861 gtcacagaag tccgctaaag tttttgtatt cactagcaac gaatataatt atgatttccc
    31921 ctatttgaac caatttttaa aagaatgtta tttagaaaaa ttgaatttaa ggatgaataa
    31981 aacatgaaaa tcgtgatcat tggtgcttcc catgcaggca tcacagctgc cctaaatcta
    32041 agaaaacttc aaccgaaagc agaagtttta ctgatcgatg aagatcataa agatggacta
    32101 ggctatgttt caaatggaat caacctttat ttaaaaggaa agatacgtag tttagctgaa
    32161 gttgcccata acatgcggac tcttcaagaa agtggggcaa aactgatcac cgaatggaga
    32221 gtaactgaac ttgatccaga caaacatcag ctcatcctcg catccaaaga aggaaaaaac
    32281 gagaccataa cctatgataa attaatcgta gcaacaggtt cctctcctgt caccttgtat
    32341 aaacaaattg aagctgaaaa tgtctacacc tataaaaatc tagtgcaatc taagcaagta
    32401 cttgccgctt taaaagaagc aaaagaagta gtgatcttcg gtgctggtta tattggattg
    32461 gaattagcag atgctttgag aaataaagga tacattattc atctagttga ttatatgccg
    32521 aatgttttat caagatattt cgataaggat atgatcaaca gttttcaaaa tcagctgcaa
    32581 actaagcaaa tcaattttta tccaaatgaa ttcttgatcg attggaaaaa atcagaagag
    32641 aaagtagtat ccgtacaact tctatctcaa gcaataaagg cagatatggt cattttttct
    32701 gcccaaactc gtcctaatac gacattgtta aaagaaaaag tggctttgta tgaggatgag
    32761 actgtgatag tcaatgaata tctgcagaca agtgatccgg acatctatgc gattggagat
    32821 atcgtcccag tcagttttga caaaaataaa cggcatctct ttttaccttt agttactcga
    32881 gctgtacata tggcaagagc agtagcgcta actttgagtg gtcagccaac cgcttacgac
    32941 ctcagacaaa agataacagc agcagtcatt atagactact ttttaggaac tgtagggctg
    33001 acagaagatg aagcgccatt cttggaacaa aataccggtt cgtgttcagg agaatttgat
    33061 ctatttccac aatacgatga agaaaacaaa acagtaaatg caaagctgat ctaccatccg
    33121 gatacattag aaataattgg cggacagctg ataagccaag aattcttatt atcagatttg
    33181 aacctgcttg cggatatcgt gaaacacaaa actacgattc cacagctagc agttgaggat
    33241 tttggatttc tgtctgaaca cacctctcgt tttcactacc tcaatgagtt agcttttaaa
    33301 gtactagcta agagagcaaa tagcaataga gtagataaaa gactgtgaga aatatcacgg
    33361 tcttttattg tgtggggata taataaatat taatcaatcg agaataaaaa attcatataa
    33421 tgaaatagcg gaagaatttt ttgagcataa tattcgccaa aaatttatca agtgataaaa
    33481 ttgttgtata aaatgattat ttcaggagga ggagttaaat gtttttagca ttaaatgaaa
    33541 ttatgcattc aaagttacgt tatgcgttag ttgcaggggt gatgtttttg atcgcctacc
    33601 tggtattttt tttgactggg ttagcttatg gcttagctca agataatcga acagcagtcg
    33661 acaagtggga ggcagattcg attgtgcttt cgaaagatgc aaattcaaat ctagggatgt
    33721 ccatgattac taagaagata gcagaagaag tcgaaggtgg gaaagtggcg tatcttgccc
    33781 agacacctgg agtcgtgact agtaaagaca gtacagaaga aggaaaaatc aatgttagct
    33841 tcttcggtat cgataaaaat caattcatca tgcccaatct tgttgaagga aaagcttttg
    33901 ataacgatga tgaggcagta ggcgatatta gtttgaaaga agaatatggt ctagccgtag
    33961 gagatacagt taagttatct ggtagtgaca agacgtttaa actaactggc tttacggatc
    34021 atgccaaatt caatgtctca ccagttctct atacaaccat caatgcttac caagagatcc
    34081 gcttcgaaaa agaagataca agtgaaaatg cacggattaa cgcaatagtt gtcagaggta
    34141 aaatcagtaa tttacctgaa gatttagaac agatcaaaat ttctaaattc atcaatgaat
    34201 tacctggata taatgcgcaa gtattgactt ttggatttat gatcggattt ttgatcgtta
    34261 ttgcggctat tgtgatcggg attttcattt atgtactgac gatgcaaaag attaatatct
    34321 ttggtgtaat gaaagcacaa gggatcacag gaggatttat tgcgcgttct gtagtggcac
    34381 aaacattcat tttatcattt gttggtattc ttcttggtct attaggaaca gtgggaacat
    34441 cccttgttct acctgatgcg gttccattcc aaagcaattg gctgtttttt ggcgtcatta
    34501 gcttgttgat gctggtagta gctgtgttag gcgcactatt ttctgttcgg acaatcgtaa
    34561 aaatcgatcc gttgaaggca attggataag aaggaggaag agcatgaaag caatagaatt
    34621 taaagaagta gaaaaaagtt tccaagatgg cgaccaagta gttaaagcat tgaaagaaac
    34681 aaccatgagt atcgataaag gggaatttgt tgcaatcatc ggtccttctg gttcaggtaa
    34741 aagtactttt ttgactttgg caggcggatt gcagacgcct agcagtggct ctgtatttat
    34801 caatgatcaa gaattcagta atcaaaaaga aagtgaacgc gttaaactac gttttaaaga
    34861 aattggcttt attttgcaag cttccaatct ggttcctttt ctgacagtaa aaaagcagtt
    34921 attattagta gataagatca aacatgaaca tcgtcaaaaa gaagcaaaag aattatttga
    34981 gcagcttggc gtggataagt tgatcaacaa gtatccagaa gaactatctg gtggagaacg
    35041 gcaaagggtc gcaatcgctc gtgcattgta caatgatccg tccattattc tagcagatga
    35101 accaactgct agtttggatt cagaaaaggc acgagaagta gtcgatatct tggcaaaaga
    35161 atcaaaagac aaaaataaag caacgatcat ggttactcac gatacacggc tgattgacaa
    35221 atgcgataga atatttatga tggaagatgg tgtattgaaa gaaaaagaat aaccaaaaag
    35281 caggtggaaa aatggtccat cgatacaaca aagatacaca gcaaaaaatt ttgaaagaag
    35341 cgactgactt gtttatgtcc aagggatatc tcggcacttc aacaagagag atcgcacaaa
    35401 aagttgggat cacccaaccc aatctctatc attatttcgg tgataaagaa aaactatata
    35461 cggcagtttt agaaaaccac ttaaaagatg ttggaaaagc tttgagagaa atcgttcaaa
    35521 caagcgaaac gggatttcaa gtaaccttga caaaaatggc gcagtttttg attgagacac
    35581 atctggtcga tttgtttctg atgcttcacg atcttgaaag caatctttca aaggaaacaa
    35641 gagatcatct tttctttcta tggaagaaaa attatcgtga accttttgaa gaaatctttt
    35701 cagcaaacca atctgtgcta agaaatggga tttcaaaaga aatcgcagca aggcattttt
    35761 tcttagtgtt agcaccttac atcactcggt cgaaaagacc tgtggaagaa ccgctaaatg
    35821 tggcgcaatt gatcgattta tatttgaatg gtgtattagg agaagcgtaa agctgttata
    35881 tagggagaaa aatagttagg tgaagaagca agatatgacg aagatcgtta tatcttgttt
    35941 tttttatata ggtagttttt cataaataca aatttgttat tctctcatga ttatcagggt
    36001 atatttttat ttattatgtc ggtgttccag gaagtgtttt aatttaataa cgaagaaatt
    36061 aattgtttgt agaatgaagg aaactattta ttgggtagat gaatcaccac gaatagtcaa
    36121 aaggagattt caggatatgc caaaaatgaa aaaaatcatt ttagcagtaa tgttattttc
    36181 tacggttata tcttttacag cccctttttt gcgtacttaa gctgatttta agttttgacc
    36241 cttagtctgg atctacgagg tgagagtcaa gaatgaaaaa aataattgga gcttttgtat
    36301 ttgcaatcat tatcattatt atctgttttc aaatagatgt ttttggtcaa aaaaagatag
    36361 ttggacaaac ggtcagcagt acgaacagta cggttacaaa gacaaaggaa tcatttgatg
    36421 atacagaaca agagacatta atcaaaatcg gaaaaaatga ccgtacacta tatacagatg
    36481 actcgctaac taccccactt gcaactataa acggcggaga gctggcggag tttctttctg
    36541 aaacaaaaaa tgcttatcag atcaagacaa atgatggcta tactggatat ttagccctcg
    36601 aagatggaac caaagtcaca aagaatatac aaacaaaacc ggaagagcta tctgatgcag
    36661 tgattgtatt agatcctggg catggcggaa atgatactgg ggcattaagc aatgatgaac
    36721 aaacagaaga aaaagatata acattaagta cagccataaa agtaaaaaag gcgctagagg
    36781 atgctggagc aacggtttat ttgacccata ctacagatga attagtacag cttggagata
    36841 tatgcgacta cagtgaagaa aaaaaggcag atgtctttat cagtttacac gcagactcta
    36901 cggaatatgc gaatgaagca actggaatca cgacatatta ttactatgga caagaggaaa
    36961 cattagcaca gacaattgct gactccttca ctgatctgcc tctggattca agaggaatct
    37021 ccactgggaa ctatcaagtt cttcgagaaa atcttcaacc ttctatttta atcgagatgg
    37081 gttatatgaa taatgatagt gatttaaagg aattagtaac agacagttat cagcagcaga
    37141 ttgctgcaag tttgacacaa gcattgacca cttattttca gtaataaata agcccagcca
    37201 aacaaaaata gctcctcata tttttcgttt ggccgggctt attatcgcag atgcaagctt
    37261 ttgaacaccg tttaaggaag aggttgtgag cctgccgttt aactccgcgt cacaaggagc
    37321 aatttcaaaa aacagcttct catgtttgat gactattgtg acttgtgacc gaggagttgg
    37381 catgagaaca ccgtttattc ggaacaaggg actgtgacat gacttttgtc acagttccat
    37441 tattttttta gcagcttcaa agctttttct gtattggcaa aatgaagttt ggcatatttt
    37501 tcaagcatag gcatgccgcc tttctccaat agttttgccg ctacttggtc agatttcgtt
    37561 ccagctcctg atggaagggt gattccagct tcagctgatg ctttgtgtaa ctcttccaaa
    37621 aagctggcac gactgatgat agaagcagca gcaacagcta aatgatattg ttctccttta
    37681 gtaacgaagt acagtttttc ttgtaattga tttttttcta aacgcgcata ttttttaaaa
    37741 tttgcttccg gcgtaaactg atcgatcaaa atcgcttctg gtttttctgg ctcgattttt
    37801 tttagcagca aatggatcgc ttgattgtgc aaagcgactt tcatccgaac ggcattgtat
    37861 tttggttgga tctcattgta tttttctgga gataagacaa gcagtttata ggggatggtt
    37921 ttttttaaga tgtcagcaag ctggatgatt tggctatctt ttaactcttt tgaatctcta
    37981 acaccaagtt tacgtaattc tgcaagctga tcacgtttca cataagcagc acatactgtt
    38041 agcggaccaa aatagctgcc atttccgacc tcatcactac ctaaaacgga taattgactg
    38101 atattttttg gcaaagaaga ggctgttttt gctggcttgg cttgtttatc ctgtgcctcc
    38161 ccccatttat cagcttctat tgctgcattc gtaccttgaa acatcacttt tccggatgta
    38221 taagcggtaa tggtcgtccc gtcttttttt gcagcaaagc tagtataagg aatctgttta
    38281 tttaacaaat aagagcgata gtatgctttc atttgactaa gttggtcgtt tgttgctttg
    38341 ataatgacgt tgctcataaa attctcctcg tttttataca taaaactata ccgcaactca
    38401 cgacttttcg ttttctaaga gattgcgaga catagcattt ttttaaagtt tatcatataa
    38461 tagttaagta tgtgaaaaat atttgtggag aacggtgaaa gaaccaactt catggtacac
    38521 taaataaaac tttcatcaaa taggaggaaa tgattcgtat ggcgcacgaa aaaacacgct
    38581 acaaagcagt gattgccaac caaacatata ctattatcgg acgggaaacc aagcatcata
    38641 tggatatcgt aacgaagctg atcaatgaac agttagcaga attgaaacaa ttatcaccac
    38701 aaatggataa tgaacaagca gcaatcttga tggctgtcaa tgcgctttcg gatcaattga
    38761 agaaacaaga aagaatcttg gagctggaag aagaaacagc agaattaaag aaaaaaatga
    38821 tcaaatttac agagcttgaa aatcgtgtga aacgcattga agcaatcgag aacgaagctc
    38881 gggaagtatt gaaagaaaat ggtcaagcag atcataccat taaaaatcat gtggaagcac
    38941 aacagatttt aaacgagaaa cgaaaaggac aaattaaaca aaaatcttct aattaagatc
    39001 cttttatcaa aataagaaaa atttaaaaaa gttagaagag aaaggaacgt agcatgctga
    39061 gtttgctgat tttattttta ttgctaattg cgtttttttc tggagcacgt cgcgggtttg
    39121 ctttgcaaat catattcgcg attggatatg tattgtcatt tattgctgcc cagcattttt
    39181 acaaaccatt agcaagtcat ttggaattat acattccata cccagctgtc accccaacat
    39241 caaagttagc attttttgat caggtatttg cttttcacct agatgaagct ttttatgcag
    39301 gaactgcttt tctgattatt ttgctaattg gctggctgct tacccgattt gtgggggttt
    39361 ttgtccatgg attgacgtat gtgccgatct tgagacaggt agactggatt gcaggaggca
    39421 tcctgtcact aatcatggct tacgttacta tatttttgat tttgcagtta cttgcctttg
    39481 ttccattaga tgtggtccaa aatcaattcg gcgagcatag tttcgctcgt tttatcgttg
    39541 agagaacgcc atttcttacc aacaagatgt atgagctttg gtaacgaata ttttgaatta
    39601 aggaaaatag tgctggaaaa tatttcgttt tttgtacaga gtagtttatt taagaaggct
    39661 gcgtagcgta tgtccggcac agctcttttt tcatagtgaa agataaggtg ataaaatgaa
    39721 taaccgaatc cttgaaacat tggaatttga aaaagtcaaa cagatggtca gacaatttgt
    39781 agtgactgca caaggaaaag aagaactggc agaattggta ccagtgagtg agaaacaaac
    39841 gatcacaaac tggcttcaag aaactgagga tggattgaaa gtgcaacgtc tgcgaggcgg
    39901 gatccctata cctaaacttg aaaatatccg tccccatatg aaacggatcg agatcggagc
    39961 agacttgaat ggagtggagc ttgcacaagt ttccagagta ttgtctacga caagcgaatt
    40021 gaatcgtttc attgatgatc tttctgacag tgaaatcgaa tttgcacgtt tgtatatgtg
    40081 ggcagatcag ttagtcacga tccctgtttt gagccgccgt ttaaaggaag caatcgatga
    40141 agacggacgc gtgactgatg acgcttctcc tgagttaaag agcataagac agaacattcg
    40201 gcgaagtgaa caagctgttc gtgaacagtt agatgggatc gtccgtggga aaaatgcaaa
    40261 gtatttaagt gatgcgatca tcaccatgcg aaatgaccgc tacgttatcc cagtcaaaca
    40321 agaataccgc ggtgtatttg gcggtgtagt ccacgatcaa agtgcttcag gacagacttt
    40381 atttattgaa ccaaaacaag tggtggattt gaataatcgt ttgcgtcaat accaaatcgc
    40441 tgaacgaaat gagattcaac gtattttaag tgaactttct gctgaacttg ttccccatcg
    40501 tcaagaaatc atccacaatg cttatgtgat tggtaaaatg gatctgatga atgcaaaagc
    40561 acgatttggt aaagaagtaa aagccatcgt gccgggaatc agtgaagaca atcatgtcgt
    40621 cttgaaacaa gcaagacatc cactgatcga tcaggaaaaa gtagtttcta atgacatcac
    40681 aatcggaaaa gactatcaag ccatcgtcat tacaggacct aacacaggtg ggaaaacgat
    40741 cacgttgaag actttaggtc tgttacaact gatggggcaa gcaggattac cgattcctgc
    40801 aggtgaagaa agtcaaatcg gtatttttga ggaagtattt gcggatatag gagacgaaca
    40861 gtcgattgag caaagtctat cgaccttttc ttctcatatg acgaacactg tagatatttt
    40921 atcaaaagtc aatgaaaaaa gtctggtatt atttgacgag ctcggtgcag gaacagatcc
    40981 gcaagaaggt gctgctctag caatcgcaat cttagatgat cttggtaaaa agtctgctta
    41041 tgtgatggca acaacgcatt atcctgaatt gaaagtttat ggatacaatc gggcaaatac
    41101 gattaatgcc agcatggagt tcgatgtgga tacactaagt ccaacgtatc gtttgctgat
    41161 tggcgttcct ggacgaagca atgcgtttga aatctctagc cgattaggtc ttgatacaga
    41221 agttattgat gaagcgaagc agttgatgaa tgatgagagc caagatttaa acgaaatgat
    41281 cacagattta gagaatcgcc ggaaaatggc agagaccgaa tatctggaaa tgcgtcattt
    41341 tgtttctgag gcacaagaac ttcacgatga tttgaaagaa gcttacagct atttctttga
    41401 agaacgtgaa aaagaaatgg aaaaagcaaa gaaaaaagca aacgaagtcg tgtcagaggc
    41461 ggaagaaaaa gcagaaaaaa tcattgctga tattcgtaaa atgcaacagc agattgggca
    41521 aggaaatgtg aaagagcatc agttgatcga tgcaaagacc caattagcaa atctacatca
    41581 agaagaaact ttgaagaaaa ataaagtatt gaagaaggcg aaagaacaaa aaacgttgaa
    41641 accgggagac gaagtgttgg ttacgactta cggccaaaga gggacgcttt tacgtaaaaa
    41701 cgggaatcag tggcaagtag aaatcggtat attaaaaatg aatgtgtccg aagacgagct
    41761 gacgcctgtt gctcctcaaa aagagccgac acaacgagtt atccatgctg tacgatctga
    41821 atctagtagt cacgtgccga atcaattgga ccttcgagga aaacgatacg aagaagcgct
    41881 aagtgaggta gatcaatatt tggattctgc cattctagct ggttatcctc aagtgactat
    41941 tgtccacgga aaaggaacag gcgcacttcg caagggaatt actgattatc ttaaaaacca
    42001 tcggagcgtc aaaagctttg agtttgcacc ggcaaaccaa ggcggaaatg gtgcgacaat
    42061 tgttaaattt aagtaaagct tactaaaggt aagcagatgg ttcgctatcc aagtatagaa
    42121 atgctatact aatcatagat tttaaaaatg gaggtctttg atatgtcaca agtaattaca
    42181 gataagacat ttaatgaaga aacagataaa ggacttgtat tgattgattt ttgggcaact
    42241 tggtgtggtc catgtcgtat gcaagcaccg attttggatc agttggaaca agaatatgat
    42301 gaagaagaat tcagaattgc taaaatggat gtggatgaaa atccagaaac tcctcaacaa
    42361 ttcggtatca tgagcattcc aacattaatg ttgaaaaaag atggtcaagt agtagaaaaa
    42421 gctgttggtg ttcattcaaa agaacaacta cgtcaaatga tcgatcaata tttataaaaa
    42481 cttacagcaa aaataagctg agggtgggac tgaatccagt tccgtcctcg gtttttttgc
    42541 ggttgagtta aggttattca aattaatatt ttttgagtat aattacgttg tcaggctgga
    42601 ttgttttttt gacagacatg ctataataac tttaattgaa tatgaagggg agtaccagat
    42661 gaacgaacga atcaaaaaca aattagcttt acttcctgat cagcccggct gttacttgat
    42721 gaaagataaa aatggaacga tcatttatgt cggcaaagcc aaaatattaa aaaatcgtgt
    42781 ccgctcatat tttactggca gccataatac gaaaacggaa cggcttgtta gtgaaatcga
    42841 agactttgaa tacatcgtta cagaatcgaa tatcgaagca ttgctcttag agatcaatct
    42901 gataaagaaa aatgatccta aatacaacat tatgttaaag gatgataaaa cctatccttt
    42961 ccttaagata acgaatgaaa aatatccccg tttggtgatt actcgtaaag tactaaaaga
    43021 caaagcactg tattttggac catatcccga tgtcggtgcg gcgaatgaga caaagaagat
    43081 cctcgatcga ttatttcctc tgagaaaatg caaacctagt cagacgaagg agccttgtct
    43141 ttactatcat ttgggacaat gtctgtgtcc ttattatttc gacgtagatc cgaaagtcta
    43201 tacgaaaatc gttgatgaag tgaaaaagtt tttaaacgga ggacacgaaa aaatcgagtc
    43261 agagatccat cagaaaatgg aaaaagctgc tgaaaacatg gagtttgaaa aagctgcaga
    43321 ataccgagac cagatccgtg ccattgagac aatcatgact cgccaaaaaa tgacaaatac
    43381 tgatttactg gatcgagatg tatttggtta tgcgattgac aaaggatgga tgtgtgtcca
    43441 agtgtttttc gtaagacaag gaaagctaat tgaacgagat gtttcaatgt ttcctttcta
    43501 taacgaagca gaagaagatt tcttgactta cctcggtcag ttttatcagg aaaatgagca
    43561 tttcattcct aaagaagtct tgattcctga tacgatcgac aagcccagtg tagaagcttt
    43621 gttagctacg aaagtgctgc aaccaaaacg aggagaaaag aaaaaattag ttgaattggc
    43681 gaataagaat gcctcagttg ctttgaatga acggtttgat ctgattgcca gaaaacagga
    43741 acggacgatc ggagcagttg aacgtttagg aaacgcgatg aatatcccga ccccaatacg
    43801 tatcgagtct tttgataact ctaatatcat ggggacgaat cctgtggctg cgatggtcgt
    43861 attcattgat ggaaaaccgg caagaaaaga atatcggaaa tataaaataa aaacagtcaa
    43921 cggaccggat gattatgcat caatgagaga agtcatctat cgcagatatt cacgagtgat
    43981 tcgcgaaggg ttgcctttac ctgatttgat cttgatcgat ggaggaaaag gacaagtaga
    44041 tgtggcaaaa gatgtcttag aaaatcaatt aggaatcgat attcctattg ctggcatggc
    44101 aaaaaacgat aaacataaaa caaatgagtt gctattcggt atagatttgg ctgtagtacc
    44161 gcttgagcga aattcaccag agtttttctt actccagcga atccaagatg aagtccatcg
    44221 attcgcaatc actttccatc ggcagctacg cagtaaaaac agttttgctt cccgattaga
    44281 tgaaattgaa gggttaggac caaaacgaaa aaaaatgctg cttaaagagt tcaaatcgtt
    44341 gaaaaatatc acagcagcca cgacagaaga attacaagaa atcggactgc cgaaaaatgt
    44401 cgcacaaaac gtatttgata aattacatca ggaatgagga aagggcactt tacaaagaaa
    44461 gtaaagtgtc ctatcatttt atgtacaaca aaaagagcgt ttacgttttg ttggtaaata
    44521 ataaatcgct tacgtctggt gtctaaggat atatgtgatt tcttgaagaa ggagtgaaga
    44581 aatgttagaa gtcaaggatc ttgttaaaac ttttgggaac tacacagctg tcgatcatgt
    44641 ctctttccaa atccccgatg gaaaaatcat ggggctaatt ggccaaaacg gggctgggaa
    44701 gacaacgact tttcgtttga tcttggattt tttgacacaa gaccaaggag aagtgttatg
    44761 gaatggccat catcttgggg agaaagatta tgatgtcatc ggttatcttc cggaggaacg
    44821 gggactttat ccgaaagtaa cgattcaaga ccaactgctt tattttgcag aacttagagg
    44881 gaaatcaaga aaagagattg aaccaaaaat cgatttttgg atggaaaaat ttcaggtaaa
    44941 agggaaaaag acggataaag taaaatcttt gtctaaagga aatcagcaaa aagtccagct
    45001 gatcgccacg ttaatccatg aacctaaatt gatcattttg gatgagccat tcagcgggtt
    45061 agatccggta aatgctgaat tgctaaaaga tggaatattg gaattgaaaa aacatggttc
    45121 atgtgtcatt ttttctagcc acaatatgga taatgtagaa aaaatctgcg atcatttgat
    45181 catgttgcga aacggaaaaa tggttttgaa tggaaaagta catgagattc gtgaatcatt
    45241 tggccgtacg aagttattct tggaatctgg tttaagccaa caggaagtag aagaaatcga
    45301 aggcgtgaaa aaagtgatcc ttcatgaaga caatacgtta gagattacct tagaagatcc
    45361 tgaagctggg aaagaaatct ttactcgtgc gactcagttc ggctacattc ctatgtttaa
    45421 ccagcaaccg ccaactttgg aagagatttt caaattgaaa gcaggtgaaa tcgatgcgta
    45481 agttttggat tattaccaaa gatgtctatt taaaaaatgt taagtcgatt tctttcttga
    45541 tcatgatatt agtccctttt atcatgctgg gtatcattta tgtagcagga aactttgctc
    45601 aacaaaatag tgatgtcaat aaaatcggcg tattggctga taatgagcaa atcagtcagc
    45661 aaatgagcca agtcaaaaca gatgattttg agttcaaagc agttgattct gaagaaaaag
    45721 cacaaaaaga gctttcggaa gaaaaaatcg atgcgtattt agtagtctca actgagaatg
    45781 ataagataaa tgggatgctt tatagcgaaa attcattagg acaaagtaca caactactga
    45841 tacaacaaca actgactggc ttgcaatcga tgcttcgagc aagtcaatta ggcatttcac
    45901 cagaagaagt agctagcttg agccaacaag caagtttgac tcgacaaaaa gtcaattttg
    45961 attcaaaagg aaaaatgaca gttggagaag ataactcgac agtccaatat gtcgtaagtt
    46021 atgtagcaac gattgtcttg ttcattatta ttttgacgta tgcgcagatc attgcacaag
    46081 agatcgcttc tgaaaaaggc acgcggatca tggaagtgat cctgtccagt acgactgcac
    46141 aaaagcattt ttacggaaaa ctaacaggtg ttttacttgt tgcattaacg caaatggcat
    46201 tatacggtat tatttttggt ataggataca gtcaattcaa agatatggaa atggtcaaaa
    46261 atatattaga cggtatctca ttggataact tgattggacc attcttgtgg tattctcttc
    46321 tattcatgat ctttggcatt ttgatttttt ctgttttagc agctttatgt ggctctttgg
    46381 tcaacaaagc agaagataca gcaaaagcaa ttttgccagt gacttatcta tctctaggcg
    46441 gatatatgct tggattgata ctaggagctt cagatccaaa caatatcgtt attcggatca
    46501 cttcgtatat tccattctta tcttcatata ttatgccggt tcgcttagca aatgagacag
    46561 tcggtatggg tggagcattg atttccttgt tggtacttgt agccgtaaca ttcttattga
    46621 tgatcttgtc tgcaaacatg tacaaatcga atgtactcgt gtatagcgaa ggcggattat
    46681 ggagttcatt gaaacaatcg atttcaatta tgagaaatga acgaaaaaaa gcataaaata
    46741 tagaaaaata tagaaggaag ctggacattt attgttccca gcttcttttt tgtatagacc
    46801 tatttctatt tttttattat attggatata tatattcatt gaaaatagag cataacaact
    46861 ttataatgtt gagtatacca atttttggag gcgttttaat ggaccgctat ttagatttac
    46921 tttcagagaa gttccctaca acggaagatg tcatcacgga aatcatcaat ctcgaagcaa
    46981 ttttgcagct tccaaaagga acagagttat ttttgagcga tatacatggc gaatttcctg
    47041 cttttgatca tattctgcgt atcggttcag gaaatcttaa agaaaagatc aaagaccttt
    47101 tctctgaaca aatgacggaa acggagatca gccaattcac tatttttacg gcttatccag
    47161 aatatgtgtt gacgactgat tggtacaaaa aacaagataa atcccagctg atccatcgtt
    47221 tgatcgatct tttacggttt acgacagtca aatacactcg atcaaaagtt cgaaaaggat
    47281 tgccgaaaga atatagctat atcattgagg aactcttgta tatcgatgat cgtatcaacg
    47341 ggaaaaaaga ctatgtaaaa aaaatcattg aacaacttgt cttgatgaag gaagaagaaa
    47401 aatttttaac aaaacttgcg cagacgatcc aaaaatctgt catcgatcat ttgcatatcg
    47461 tgggtgatat ttttgatcga ggaactcaag cagccaaagt aatggatcga atcatggatt
    47521 tttcctctgt agatatccag tgggggaatc atgatatttt gtggattggt gcgtattgcg
    47581 gctctgaggc atgtttattg acgcttttac ggattgccgc acgctacaac tatttatttg
    47641 aattagagaa agaatatgga ttaaatttac gtccattatt ttcttttgcc catcaaacct
    47701 atcagaagaa tccagtattt acccctaaaa acaggaaaga tctatctgaa agagagcaag
    47761 aagaattaga aaagatccat caagcacttt ctatcctcca attcaaatca gaacatcagt
    47821 tgttgaatcg acgacctgaa tttaaaatgg acgatcgtaa attgttggaa aaaatcaact
    47881 atgaggaatc aacgatcgtt ttagaaggtc agctctatga gttgaaaggg acttgttttc
    47941 aaacgatcca gccagatgat cctaaaaaat tcttggaaga ggaacaaaaa gtgatcgata
    48001 gtttgatgta ttcattccag cactcgttaa gaatgcaaaa acacatgacg tttttgatcg
    48061 aaaaaggggg aatgtatctc acgaacaatc agcatgtact gttccatggc tgtatacctg
    48121 tagatgaaaa ggggcagctc ttagcatttg aattagacca atcttatcgc ggaaaagaac
    48181 tgctgggatt ttttgaaaaa cagataactg aaagcgcaaa agatttgact gcaaatgaag
    48241 atttggcgac cgatttgatt tggtatgcgt ggagcgggcc attttcgcct ttattcggca
    48301 agagtaaaat ggcaactttt gagcgctatt ttctcacaga gcctcatacg cacgtcgaaa
    48361 aaagcaatcc ctattatcac ttgcgagatg aagagtggtt cagtgaaaaa attttggcag
    48421 aattcgatgc aaaagaagaa ggttcagcaa tcatcaatgg acacacgcca gtaaaagtaa
    48481 aaaaaggaga gtcaccgatc aaagcaaacg gaaaagcatt tgttattgac ggaggtctat
    48541 ccaaagctta tcaaaaaaca accgggattg ctggctactc attactatgc aattcttacg
    48601 gtttccaaat cgtgacacat tatccgttct tgcctattga acaacaattt gcaaaaaaat
    48661 ctgatcaaac gaatgtaaaa aaagtgatcg agcagcagct cccacgcaaa cttaatcgga
    48721 atacgacaaa aggaatggaa ttacagcaac agattactca actccaaagg ctgttggatt
    48781 atcgaaagga cgattgagaa aaatgctagc caacattgct caatagacta aaaagcgact
    48841 gcaacaaaag gcgtgtcgca gtctccttcg gaattagctc aaaaaacggt aaaatattag
    48901 aagctatttt tctgtttatt tctctaatac tcaggtcagg gcgctgattt cacgacctct
    48961 tccttaaacg gtgttctcct gccaactcct cggtcacaag tcacaatatc caacaaacat
    49021 gagaagctgt tttttgaaat tgctccttgt gactcagagt tgaacggcag gctcacgacc
    49081 taatcagaaa aacgattaaa attcattcac aaagaaatcg aataaacgct gttcagatga
    49141 atctacacga tgagaaagtt ccggatgcca ttggacacca aggattttgt gcatattatc
    49201 tttactttgg actgcttcaa tgagtccatc gttggaaaag gcgatggctt ttaacaaagg
    49261 agaaagatct ttgactgcct gatgatgata ggaattcaca aagtatgaat cagggagaag
    49321 gtcacttaat aaactgtccg aaacgatttt gacttcgtga gttgcaaact gcggggcagt
    49381 gggctgttgt tcgtgtttta cggaccattt tggatataat gaaagatctt gataaagtgt
    49441 tccctccaac acgacattca atagttgaag tcctcggcag actgcaaaaa tcggttttct
    49501 ttgtttcaat gcttccaaga tcaatgcctg ttcgaattgg tctcgttgaa tatttgtttc
    49561 cgtcagtttt ggatgagggt cttccatgta aagttgcgga ctaacatcct gaccgcctgc
    49621 aagaatgagt ttgtcgattt tctcaattag ttgaggcgct gaatctggag acatgactgg
    49681 cagaataaga ggtaatcctt gagcatctaa aactgcatcc acaaatccct gcggtgtata
    49741 gcttacttga tttccttgga aagtatctgt cgctcgaatc agttgattcc cggcaatgcc
    49801 gatgattggt ttcattttta ttccccctaa agtagactaa ttgtaacaag gatttaaaaa
    49861 aatttaaact atgtaagact tgcccctata cttatctgtt cttgctgtgt tactatctgt
    49921 aaagagaatt ttcgtgtgaa attcatgacg atagttgaaa atattgctta atttgttata
    49981 ataaaaagta agcagaaata aaaaggttgg gcgtaagctt caaggatcta gttcttcaag
    50041 gggtgagaag gacattttat tgaaggggcc tgcctatact aagttatctt tagaggtaca
    50101 tactttcaat gaaaaaagaa agcatgtacc tcttttttaa atcgaaagga agtgaagcaa
    50161 gtgttagaag ctttggaaca aatccgaaaa gcagaagaag aaaatgagaa gcgtgctcga
    50221 accctacggc agcagttgaa gacctatgca gaagaaaaag agcaagaatt ggagataatc
    50281 caaacgaaca gtcggcaaca gctgaaacag ttggtagaag aaaaagaaca attcgaaaag
    50341 caagaattag aaaaagaaaa aatgaaattg aaagcagaag cagacaaggt gcaagcaact
    50401 ttgaaagacc aatatcaaaa gcatcagagt caagcaatcg gtgcaatcat tgaaagggtg
    50461 aaagaaactt atggccgtca ctaagatgga aaaagtgacc ctcatttcag ataaaagaaa
    50521 tcaggagagg attttacaag cagtacaagg aatccaaaat gttgaaatcc gggatctgta
    50581 tcaaaataga acaaacaacc agtgggtaga aaaatatttt ccagatacca cgattattga
    50641 taaagaagca aaattaagcg ctttaggcaa tcggctgaaa gacatccaag aagctatcca
    50701 attcatacaa caccacggcg atagccgtca aaagaaactc catttgaaac gtcaagaact
    50761 ttccttacaa gacttggaag caaattattc cgaagaaaat ttcatgaata aattagaaga
    50821 agtccttgat ttaaaagaaa agtgggaaca gctaactgaa aaacgtgatc gtttagaaga
    50881 agaggaagaa tggcttcttc attggcaaca gctggatatt gctccaagta tgtatgcaag
    50941 taagacgacc atttttgaga tggggactgt gagtgcagca aattttgaaa gtttcaagga
    51001 agaactaagt aagcttgatg acgtttatat ggaagaaata gattatacgc caaaacaagt
    51061 ttatctttcg tatgttgctt taaaaaaatc agttgctgct atagaagaaa ttgctgggaa
    51121 atacgggttc aataaagaaa cgtatcctta tgaacaaaat cctaaagaaa aattgcaaga
    51181 aaccaaacag caattatcca ttgttcacga gcagcaaaaa aagataacga cagcgttagg
    51241 tcagtgcagc ggctatatcc aagaatttga atgggtagaa gaagtcacac ttgccgttgc
    51301 agaacgggaa aaagtaaaag aacggtttat ccatgccact tatctgatcg ttttgcaagg
    51361 ttgggtagat gcagaagaaa aacagaattt gatgcatgtg ttaaacgaaa ctgtatcaga
    51421 agaagatata tacgttagtt tcgaaacacc aaactcagaa gagattcagc aggaagtccc
    51481 tactaaattg aaaaaccatc cgcttatcga accttttgaa ttgctgactg aaatgtacag
    51541 tctgccaaaa tatgaagaag tagatccaac gccttggatg acaccatttt atcttgtctt
    51601 cttcgggatg atggtcgcag atgttggtta cggattgctg atgctcattg ggacaatact
    51661 agctcaaaaa ctatttgttt taccccgcgg gatgaaacgt ttcgtaaaat tctttgagat
    51721 tctctcggta ccatcgatca tctggggatt gatctacagt tcatttttcg gacaggcatt
    51781 accaaaagaa ctatttggta tccgcttgcc tttcccaatc ctatctacga cggatgacgt
    51841 caatacgatt ttgattttat ccgtgatttt cggtctgata cagatcttgg ttggattatt
    51901 cgtggcagca aaggaaaata tcaggaggaa agattacttg agtgctgtca gtgatggttt
    51961 tgcctggcaa gggatcctga ttgggatcgt tattgcattg attggtgtca tgttgataaa
    52021 aaatacagca tttgtttatc ttggcgcggg gctatcgatc attgctgctt tatgtatctt
    52081 aatcgtgccg attatccaat cgccgtctaa agtcaagggt gctgccaaag gagcgtataa
    52141 tctttacgga ttgactggtt atatcggtga tttagtcagt tatacacgtt tgatggcact
    52201 aggtatttca ggcggaagta ttggcgcggc attcaatatg ctagttgctt tcatgccgcc
    52261 agccgctcga ttcagtgtag ggatcctttt gatcatcgct ttacacgcac tgaatatgtt
    52321 tttgacactg ttaagtgcct atgtccatgg tgcacgattg cagtatgtcg aattttttgg
    52381 aaaattttat acaggaggcg gacgtgcctt tgacccgctg aaaacggcgg aaaaatacgt
    52441 caacatcaat cataagaaaa aaaacaataa ataattggag gatttttata caatgatgga
    52501 ttacttaatt agtcaaaatg gtggaatggt atttgcagta ttagcgatgg caacagcgac
    52561 gattttttca gggattggtt cagctaaagg tgttggaatg acaggtgagg cagcagcagc
    52621 gttaacaaca agtcaacccg aaaaattcgg acaggctttg attctgcaat tgcttccagg
    52681 tacgcaaggg ttgtatggat tcgttatcgc ctttttgatt tttatcaact taggaaatga
    52741 tatgtcagtt gtacaaggat tgaactttct aggcgcttct ctgccgattg cttttacagg
    52801 tttgttttca ggtatcgccc aagggaaagt tgcagcagca ggtatccaaa ttttagcgaa
    52861 aaaacctgaa catgctacaa aaggaatcat ttttgctgcg atggttgaaa catatgcgat
    52921 tttaggtttc gttatttcct tcctattagt attgaatgcc taagcataaa ggaggaaaag
    52981 cagatgaacg ccattgaaaa aatcatctct cagatgaatg aagcagcaga gcaagaaaga
    53041 gcggcgttag agcaagaaga gcgaatgaag atcgatcaaa actttgaaca aaaacgtacg
    53101 caaatcgaaa cagaacatca aaaacaaaaa gaaaaacaaa tcgaattact tgaaaaaaaa
    53161 taccgtcagc ttaggaatcg ccaacaagta gaagtacgac aagaaaattt gaatgcgaag
    53221 caggaatttt tacgacgatt gtttgcagat gcaatagctg aaatggagaa ttgggatgaa
    53281 tctgaacaaa tccagtttat caaaaattca ttgtattcat tacctttgac aggcaaagta
    53341 acattcattg ctggagaaaa atcagcagct tatctttctc agacattgct ggatgaatgg
    53401 aacaatgaac ttccctttat gatggtttta agtgatgaaa ctgttgctga tcaagcgggt
    53461 tttctgataa acgatcaagg agttcagtat aattttcttt tcagcagcct tgtacaagat
    53521 atccaaggaa cgatgagttt tgagattgcc aatcaattat tcgaataagg agggatcagt
    53581 gtatggaata ccacgaacta aatcccttga ttcgcggaag agagcttgaa cttttgtcca
    53641 atgagacatt tgaacggatg attcaaacaa attctgtcga agcacttggt gaaatattga
    53701 aatccacgat ttacagtcca tatatttatg acggatatga atatgacttc gaacagaatc
    53761 ttttaaagga gcagagccgt ctatttcgct ggttaaagga atcagcaccg gagcctgaaa
    53821 tcgtttggat ctatacgatg cgctatactt tccataatct gaaggttttg acaaaagccg
    53881 agatgacaaa tcaaaatctg gaccatttgt atatcgacga tggattttat tcattagaaa
    53941 ccttgaagga tgccattcat acgcaagttt caaatgaact ccctgaaagc attatggaca
    54001 gtatacgaga agtacgtgat tattttgaag agtctaccat tcttcaggga attgacgtga
    54061 tttatgatcg acagtttttg acagagcagc gacggcttgc cgataagcta ggatatcaag
    54121 aattgctgac agaagtcatt gcttttattg acttgaccaa tatcacaacg atggctcgag
    54181 gaatattgca gaaaagaaca tatgggttca tgacgacggt actttccagc tcgggaagta
    54241 tcgaaaaaga agtatttttg aactttgtag ataaagatat ggaagattac acgaattttc
    54301 tattagagac agattatgct gattttctgc gccctgtcat ccgtgaggga aatcttgatc
    54361 tcgtaaagct tgaacagttg aaagatgatt atctaagttc attgtatcaa gaagcacaaa
    54421 cccaggcttt cggaccactg ccactactag cctttttgaa cgcaaaagag gtagaaagca
    54481 aaaaccttcg tttactggtt atcggcaaaa gaagccattt cgaaccagaa acaatcagaa
    54541 agcgggtgag acaagtctat gacgcataag atcggtgtag ttggcgacaa agattctgtt
    54601 ttgccattca aattgtttgg ctttgatgtt cgttatggta caaccaagca aaccgttcgt
    54661 caggcaattg aagaaatggc aaagaatgag tatggcgtga tttatgtcac agagcaatgt
    54721 gcagcattga gcccagaaac gatcgaacgt tacaaggaac aaatgatacc tgctatcgta
    54781 ttgattccca atcatcaagg atcattaggg atagggctgg aagaaattca aaaaaacgta
    54841 gaaaaagcag taggtcaaaa tatcttatag tatgaaaagg agatagaaat tttgcaaatt
    54901 ggaaaaatca taaaagtctc cggacctctt gtaatggctg agaacatgtc tgatgccagt
    54961 atccaagaca tgtgtctagt gggagatcta ggagttatcg gagaaattat cgagatgcgt
    55021 ggcgatgtgg cgtctattca agtatatgaa gagacttctg gaattggtcc aggcgaaccg
    55081 gttcgctcta ctggtgaagc tttatctgtt gaattaggac cagggatcat ttcacaaatg
    55141 tttgacggaa tccaacggcc attagatacc tttatggaaa tcacacaaag taatttctta
    55201 gggcgcggag ttcaattgcc cgcattggac catgagaaaa aatggtggtt cgaaccaaca
    55261 gtagaagccg gagaaacggt gagtgctgga gatgttatcg ggatcgtaga agaaacaaaa
    55321 gttataaaac ataaaatcat ggtgccaaat gggataaaag gaacgatcaa aacaatcgaa
    55381 aaaggttcat tcactattga tgaaacagta tgtgtcattg aaaccgaaaa tggtgacaaa
    55441 aatttgacga tgcttcaaaa atggccagta agacgtagtc ggccaatcaa agaaaaatta
    55501 aacccggatg cacccatgat cactggacag cgagtgattg atacattctt cccagtgaca
    55561 aaaggaggag cagcagctgt acctggtcct tttggtgcag ggaaaacagt cgttcagcat
    55621 caaattgcaa aatgggccga tgtggacatg gttgtctatg tgggatgtgg cgagcgtgga
    55681 aatgagatga cggatgtttt aaatgaattc cctgaattga ttgaccctaa tacaggagaa
    55741 tcgttgatgg aacgtacggt attgattgct aatacatcaa acatgccagt agcagctcga
    55801 gaagcttcta tctatacggg aatcacaatt gcggaatatt tccgtgacat gggttatgac
    55861 gttgctatca tggcagactc cacttcacga tgggcagaag cacttcgtga gatgagcgga
    55921 cgtttggaag aaatgcctgg tgatgaaggt tatccagctt atcttggttc acggcttgcg
    55981 gaatactatg aacgatctgg aagagtcatt gcattaggat cagaaggtcg cgaaggaagt
    56041 ataaccgcaa tcagtgctgt atccccgtct ggaggggata tttcggaacc agtcacacaa
    56101 aatacattgc gggtcgtaaa agtattctgg ggtcttgatt ccagtttagc ccaaaaaaga
    56161 cattttcctt cgatcgattg gatacaaagc tattctcttt attcaacaga agtagggaag
    56221 tatatggatc atatccttca agctgactgg gcatccatgg tcaccgaggg catgcgtatt
    56281 ttgcaggaag aacagcagct gaatgaaatc gtgcgtttgg tagggattga ttccctttca
    56341 gataatgacc gtctgacatt agaagtggca aaatccattc gtgaagatta tttgcagcaa
    56401 aatgcttttg atgatgtcga tacattcact tcaagagaga aacaattcaa aatgttgaag
    56461 atgatcttga cctttggtga agaaggacgc cgtgcgttgt cgttaggtgc ttatttaagt
    56521 gagatcatgg acggtaccgt atcgattcgt gaaaggatcg gacgaagcaa atatatcccg
    56581 gaaacagaat tagaaaaact agatcaaatt attggtgaaa tcaagcaaac gatgcaaacg
    56641 atcgtttcag aaggaggcat gagcaatgat taaagaatat cgtacgatcg gtgaagtcgt
    56701 cggtccattg atggcggttg aaaaagttgc tggtgtcaaa tatgaagaat taatcgaagt
    56761 ccgtatgcaa aatggcgaga ttcgtcgtgg acaagtattg gaagtccaag aagataaagc
    56821 aatggtccaa atattcgaag gaacaagtgg tatcaattta cgtgactctt ctgtacgatt
    56881 tttaggccat cctttagaac taggtgtatc ggaagacatg atcggtcgag tattcgatgg
    56941 tttgggacga ccgaaagata acggaccaga gatcctgcct gaaaagcatt tggatattaa
    57001 cggagaagta atcaatccag tagctcggga ttatccagat gagtttatcc aaacaggtat
    57061 ctctgcaatc gatcatttga atacattagt tcgtgggcaa aaattaccag tattttctgg
    57121 ctctggtctt cctcataaag aattagctgc tcagatcgcg cgacaagcaa cggtactcga
    57181 ttcatcagat gatttcgccg tagtctttgc tgctattgga atcacttttg aagaagctga
    57241 atttttcatg gaagattttc gtcaaacggg cgcaatcgac cgttctgtca tgtttatgaa
    57301 tttagcaaac gaccctgcaa tcgaacgtat cgctactcct cgtatggctt tgactgctgc
    57361 agaatatctt gcttatgaaa agggaatgca tgttttggtt atcatgacag acatgaccaa
    57421 ctatgcagag gcattgcgag agatttctgc agcacggcga gaagttccag gaagaagagg
    57481 atatcctggg tatctttaca cgaacttagc tactttattc gaacgagcag gaagaatccg
    57541 cggactaaaa ggatcggtta ctcaaattcc gattctaaca atgccagaag acgataaaac
    57601 tcatccaatc cctgacttga ctggttatat tacagaagga cagatcatct tgactagaga
    57661 gctttacaag agtgggattc agccaccaat tgatgtactc ccgtctcttt cgcggttaaa
    57721 agataaggga actggacccg gaaagacaag agaagaccat gcagctacga tgaatcagct
    57781 ttttgcagcc tatgcacaag gaaaacaggc aaaagaatta gccgtagttt taggtgaatc
    57841 tgctttatct gatgtagaca agatctatgc gaagtttgct gagcggtttg aaaaagaata
    57901 tgtcaatcaa ggattctaca cgaaccgttc gattactgaa acacttgatc tagggtggga
    57961 actgctatcg atgttgccaa gaaccgaatt aaaacgaatc aaagacgaca tgctggacaa
    58021 atatctgcca gaagggaagt gagttctgat ggcacgattg aatgtgaatc cgacccgcat
    58081 ggaattgact cgattgaaaa aacagttgac aactgccaca agagggcata aattattaaa
    58141 agataagcaa gatgaactga tgcgtcaatt catcctgttg attcgaaaga acaaccagct
    58201 gcgtcaagaa atggaagagt ctatgacaga agcaatgtct gattttgtat tggcaaatgc
    58261 ttcgatcacg gaacctttca tcgaagaatt atttgttttg ccggcggaaa acgttgattt
    58321 gtctatcgta gacaaaaata tcatgagtgt aaaagtacct gtgatgaatt ttgaatatga
    58381 tgaacagctg acagacacac ctttggaata cgggtatttg aattccagtg ccgaactgga
    58441 tcggtcaatc gatcggttta ctaccctgct tccaaaactt ttggaactga cagaaatcga
    58501 aaaaacttgt caactgatgg cgagtgaaat cgaaaagaca cgtcggcgag tcaatgcatt
    58561 agaatatatg acgatccctc aattagaaga aacgatctat tatattcgta tgaagctgga
    58621 agaaaacgag cgcgcagaag taacaaggtt gatcaaagta aaaaatatgg gtaccactga
    58681 ctaagtggta tcttttttta aaagaggaaa acgatgatta aacaaagcaa acaaataaag
    58741 aaacgtttgc cggctgttca gcttctcgca ttaggatttt ttctcctgat tttagttggc
    58801 ggcggcctac ttaccctacc attttttagt aatagtgggc atgggacaaa ttttatcgat
    58861 gcgcttttca ctgcaacttc tgctgtctgt gtaacgggat tgacaacatt gaatacagca
    58921 gagcattgga acagtgctgg tcaatttttg atcatgcttt tgattgagat cggcggtctt
    58981 ggttttatga tggttccgat tctatgcttt gcattgataa agaagaaagt cagtctcagc
    59041 acacgtatta tattaaaaga agcgctgaat ctagaagaag tatcaggtgt gatgaagctc
    59101 atgctttaca tcctaaaatt ggcctttgtt atccaattga ttggtacggt tgctttatct
    59161 tttgtatttg tgcctgaatt tggctggttg aaaggtatct ggtacagttt gttccatgca
    59221 atctctagtt tttgtaatgc agggtttgat ttgttaggag acagtttagc caatcaccag
    59281 caaaatgttt atttgatcat ggtggtatct gctttgatca tttctggtgg gttaggcttc
    59341 atcgtatggc gtgatttact ggaatatcat cagaaaaaga agatgacgat tcattcaaaa
    59401 atcgcattaa ccgttacagg taccctttta ttaggcggat tcgttgtgtt ctttttcaca
    59461 gagcgcaatg gggcaacgct agtggaaggg aatatgtttc aacgattggc aaacaccttt
    59521 tttatgagtg tgacacctag aacggctgga tattattcag ttgattacct ccatatgagc
    59581 catgcaggtc tgattttaac gatgtttttg atgtacatcg gcggaacatc tgggtcgacg
    59641 gccggcggat tgaaaacaac tacgttaggt gtgttgctga tccagatgcg ttcgatgttg
    59701 aaaggacgta cgagagcgga agcatttggc cgtaccatcc gacaaggcgc agtgttgcgg
    59761 gcacttactt tattttttgt tacgttgacg ttgtgtgtag tcgccatcat gattttatcg
    59821 gtgactgaaa cgatacctaa aacttctggc atcgaatata ttgcttttga ggtcttctct
    59881 gcgtttggta cagtaggtct tacgatggga ttgacgccag acttgactgc aatagggaaa
    59941 ttgatcatta tagcgcttat gtacattgga cgagttggtg tattgactgt ggtcttctct
    60001 ctgatgacaa aagcacaaag aacacaatca aaatacaaat atccagaaga aagtgtcatg
    60061 attggataat ttcatcttct gacagaaacc tagtgattta gtaaaataag aaaataaaat
    60121 aatttttctt gcttcttgga tactttaagt gcaaaatgtt cgtttatttt ttgaggcact
    60181 acccgttgtt gaacctcggg tagtgcctct tttttattga aaggaatcaa taagatttga
    60241 ttcctttatt tatcaatagg ttataattca tattttttta ttttttcttt tacgatactt
    60301 agtccagaag ctaaaatttc aaggttatta gaggataggg aaattcgaac gaatgcatct
    60361 ggttgcgcac gtgtagtaaa gcgtctggaa tgataaagtc ggataccgtt agcaagaaag
    60421 tcattctcaa ttatcttagg gtcaatctca ggatcaactg gaagaattcg atagtaaggg
    60481 acacgattcg ttggtttttc tactttgaaa agagaatcaa aaagctgatt ggctcttttt
    60541 gtaagactta atttagtttc taagatccta tttgcttcat ttgaatggag gatttgagtg
    60601 atgacttcca catctaatcc agaagttttc acatttatat taaaaattgc ttgttctatc
    60661 ttgctttgga catgtactgg aactacaaga taagcaactc gcaatccaga acaaataaat
    60721 tttgtcattc cagctaagta gattgtttgg ttaggtaata aattttggaa aggaggaatg
    60781 atcgtatgtt gatagtaagt tgttaaaaat gaatggatat catcttcaat aacccataga
    60841 ccttcttttt caatcacttc ggctaattgc tctcgtcttt tgtgagagat ttgaaaccct
    60901 ataggattat tacaagaagg cataagaaag acaccatgaa tcttcttttt tctacattcc
    60961 tgaatcaata tatcaggtcg cattccttgc tcgtcaaaat caattggaac gatctctaaa
    61021 tgatataact gagccaattc aatgaaatta gcgtatgtat atcggtctgt tgctattcta
    61081 tcacctggag agaaaagtgc agaaagtgtg atagccaatc cattttgaac accggaaaca
    61141 atagatagag attctaagga cggaatgact ccttgagttt ttaaccaatt tgctgcagta
    61201 gtcaattgaa aatttgtgcc tttgggatct tggtaattaa gcaaaccaac aagatttggg
    61261 ttattactag ttgattgaat aaacgggaca acgaatttat tacattcttc aaaagaacta
    61321 accagaccaa aatcaatgat atgttcggtt acttcattgg tagagatggt gatggactca
    61381 aagacattgg gagaaataaa tgtgccactt ccaatattcg tatacagcaa acctttctca
    61441 attccatatt tataagcctt gccaacggta gtaaaattta aatctaaata atcagctagc
    61501 tctctttggg agggtaactg agtgtttttc tgtaatttat tctttaaaat atcttgttct
    61561 aattgttgaa tcaacgatcg ataataagga cgcttcaatt tttgtttgtc tggtcgccaa
    61621 gttaacgggt aattgtcata agaattaata ggcattttct ccactcctta aaattgtata
    61681 ccatacaatt ttaccattga ttgtattgat tttagaagtt aatcttttgt gaggagggaa
    61741 ttgtctatga aaattattaa ttttgaggaa gtagtagctt cttttagttt tgaagaagcg
    61801 attcaagtaa tgaaaaaatg tttttttgat tatgaaagtg gtaaaatcag ccaaaatcct
    61861 cgattggtgg aaattttacc tgacggaaaa ggcaaaaata tgtttgcgat gatgcctgct
    61921 tatttggggg agaacagata ttttggcaca aaagtcatta ccgcttttcc ggataatcat
    61981 cagtatcatt taccttctca tttaggagag attttcttgt ttgatgctca aaacggacta
    62041 cccaaagcat tgataaacgc aaatgcagtc acctggattc gaacagctgc tgtatctgcg
    62101 ctggcaacag attacttagc aaatccgcat gcagaaacac ttgctttaat tggagctggt
    62161 caacaagcat tgtcgcattt gactgcgatt tctaccattc gtcccatcac tacagtgttt
    62221 gtttacgata ttttagaaga aagacgaaat acttttatcg ctaaagctaa aaaacagtat
    62281 cctaaaataa cgtttataaa tgcaatatca gtagaagatg cagtacgtcc agcggaaatc
    62341 atttgtacgt tgacctctag taaagaggca tttcttcaaa aagagtgggt aagggaagac
    62401 gcacatatca atgctgtggg cacatttact cctgatacaa gagagataag tacatcgttg
    62461 atggaaactt ctcaaatcta tgtagatgat tactcagctg ccttaacaga aagcggggac
    62521 ctgcttatag ccatggcaga aggagattta gaggaatctt gtattgttgg tacattaggc
    62581 gaattagttt ctgagaaaaa aacattttat aaaggagaat cgaggccgac gattttcgac
    62641 gcagttggtc tagctattga agatctttgt tgtgcagaat atatttataa caaattagag
    62701 aaaggggctt taaaatgaac ttagattatt acgtagttga tgcatttgca gatgaggtgt
    62761 tcaaaggaaa tccagcagcg gtttatgtgc tagaagaatg gctgccagaa ggtacgatgc
    62821 aaaaaattgc cattgaaaac aatttatctg aaacagcatt tacagtgaag aaaaaccaag
    62881 aatttgagct tagatggttt acgccagatc gagaaatcga tctttgtggt cacgcgacat
    62941 tagcaacagc atttgtttta tttaactatt acaaaatacc tgatgaaacg attaaatttt
    63001 ctactcaaag cggtaatcta tttgtgacaa gaattaaaga ttattattac atggattttc
    63061 ctagtattat gccgaaaaaa gtgccgatat tgtctgaata tgaagaagcg attggggcta
    63121 agattaaaga agcctactta gcacgtgatt tatttttcgt attagaagat gaaaaaacgg
    63181 tggctaaatt gcagccagat tttacagcta tcaaaaattt tgaattagga ataggagtca
    63241 tcgtgacagc agaaagtgtg caaaaagact ttgtttctag aaccttcttt ccaaaattaa
    63301 caatcaatga agatccagta tgtggatcag cacattctaa tcttattcct tattgggcag
    63361 aaagaatgaa taaaacaaaa ttgtcagctt atcaaatgtc atcaaggggc ggggatctag
    63421 aatgtgaatt aaaagaagat cgtgtgataa taggaggaaa agcagttttg ttttctaaag
    63481 gtacctcgtt tatttgattt ctttcaaatg aattaaaaat aaattggtgg agagtggcaa
    63541 ccttatagct acaatggttg ttaaaaaaag acgcaaactt tttcaattta atgaaaagtt
    63601 tgcgtctcat tttaattatt ggtgtttgtt ttttattcaa tcaagagtaa aatgccccat
    63661 actaactatc ctcggatgac ttcgatttta taaccatcag ggtcaatgac aaagtagtaa
    63721 gaaggcgcag tgccaggtaa tccttttaag tcagtaatct caagaccggc agctttatgt
    63781 tgctcgtgta atttttctag atcatcagct gctatagcga tatgaccata gccgtctcct
    63841 agatcatacg caccatgatc atagttgtaa gtcaactcta attcatagtc atccccagga
    63901 agtgtcaaat aaacaagtgt aaatttgttt tctggaaaat cacggcggcg gctttcttca
    63961 aaaccaaatg ccttttgata aaattcgatt gatgcttcaa gatctttcac tcgaacacat
    64021 gtgtgagcca ttttcataga ttcatctctc cttttccttt tactcaatca taacaaaaag
    64081 aagtaaggaa atagttgatt tgtgcttgtt catgagaaat ttctcatgta aaatggatag
    64141 aaaataaaac tgttaaggtg gaaactattc atggataaac caatttttgg tgaaaaaaag
    64201 caatttgctg attatcaaac ccgttatgca gcatatattg tcatagaaag agatcagcag
    64261 attgcagtaa tcgaggctcc aaatggtgct ttttttcttc ctggcgggga aattgaagga
    64321 gaagagacga aagaagaagc gattgtccgt gagctgatag aagaaatggg aattgtggca
    64381 gaaatttctt cttatctggg agaagctgat gagtattttt actcgaatta tcgtgcaact
    64441 tattattata atccaggata tttctttgtt gctgattctt ggaagcgtat aggagaacca
    64501 actgaaaaga ccaataaaat ttggtgggtg agccctcaag aagcgcttgt gaaattgaaa
    64561 cgtggtagcc accgatgggc agtcgagaga tggatcgaaa aaaataaaaa ctgaaagaaa
    64621 gcgagtagat cagctttctc tcagtttttt tatttattac agtacatatt ttttgattgc
    64681 ttcagctaca ccgtgctcgt cgtttgatga agtgatgaca tttgctgctt ctttgacaag
    64741 agaaacggca tttcccattg ctactcctag accagcatat tcaatcatgg ataagtcgtt
    64801 ctcgttatct ccgatagcca tgatttcatc ttgatcaata ccaaggtgct gtgcgagatt
    64861 cgcaactgcc gcacctttac ttgcttcttt atttagtact tcgaagtaat agggcgcact
    64921 tttgaccgtt gtgtatttat cacggaaagt ttgcggtaaa cgcgcaattg ctgcatctaa
    64981 aatttctggt tcgtcgatca tcatcatttt gacgatgttc atgtctggtg tcatttcttc
    65041 aggtgttcga tattttagcg gcatcttgac taaacttgct tcgtggatcg tataagcgct
    65101 gatatctcga ttaggcgtgt agatcgcttt gtcatcgatc gaatggagat gactgccgac
    65161 acggcgtgcc atcacttcga tctctaggaa atcttcatgt gtcagtccgt gacgggcgat
    65221 gattttacct gatttcgttt gttgtacgag tgccccgtta taagtaataa cgtaatcatt
    65281 atcttgaaaa aggttcaact cgtttaactg ttcctggaca cctggtaagg gacggccagt
    65341 gcataaaacg atcgaaacac cttttgcaac agcagcggca atcgcttctt ttacttcttt
    65401 tgttacttct cttttctcat ttaataaagt accatcgatg tcgatagcga ctagtttgat
    65461 agacataatt tcctccaata tattttaatt tatttcaatc aatgcgccat tacggatatg
    65521 gctggcaaat tcttgataag tttcgtcaaa taaatcataa tgatcacgca aggacggatc
    65581 aaccatttct tttggaaaga aaaatcgctc atctccttgt tcttgtcccg ctaatgcagc
    65641 aacaagctga ctaacctgtg ataattctat caaagttccg tcatttcgca aaatctcgat
    65701 ttgtgtgcga tgtctgcctt gttctgggcg ataaaaatca taaggtagat cgtagctaga
    65761 attcacagca gtgtaataaa caggattata tccgaccttt tctactaaca aagtgagttc
    65821 ttggactaat tttgaatccc ggttgccaga aaaagtagca gatttcagtg gtttacgatt
    65881 caaaaaacgt ttagctaaat catttaaaat aggatcagca gattcagacc attgagtaaa
    65941 gtaagtgccc aagaccccat catcaagttt gaggtattct tctaaagtaa agcttccgtc
    66001 aagaaatggc agaagtaatt gagaatggag ttcaaaactg ctggcatctt cttgatacaa
    66061 ctcgtgtgcg cgatgaagca gatgctccaa aattacttcc ataccacggg aaacaggatg
    66121 gaagtacact tgcacataca tttgatatcg gctaacgata taatcttcca ctgcatgcat
    66181 gccactcatg gaaaaagcga tcccgccttc ataaggacga atcacacgta atattcgggt
    66241 aagatcaaat gttccatatt ctgttccagt gaaataagca tcccgaagca aatagtccat
    66301 tcgatctgca tcgatctgac tagaaatcat ttggacaact tgtgggttgg ggtaagtttt
    66361 ttggatcaca ctagctacct tttcaggaaa accttcttct actcggttaa gaatctgata
    66421 cacttctgtt tctggagaag tgatgatctc tacagtgatg gcttcatgat tcgtatggaa
    66481 gatatgttca aatgtatgag agtaaggacc atgaccgaca tcatgcaaca gtgccgcaca
    66541 taaagtcacc agccgttcct gatcatccca gcctccgttg cctatttttt cttttgaaaa
    66601 atttcgagaa aagatatcgc agattcgtct tgaaatctca taaacgccta atgaatgagt
    66661 aaaacggcta tgttctgctc cgtgaaaagt gaaagaagaa gtgcctaatt gtttaatgcg
    66721 tcgtagacgt tggacttctt tcgaattgat caaatctaag attacttgat gctggacatg
    66781 gatatagtta tggactggat cacgaaaaac tttttccata ggcagcattt gatatttata
    66841 aggggttgtc ataatgtagc tcctttatgt tattacgttg ttccattctc tggatttggt
    66901 tgttccattg ctccagctgg atttcttttt ttagccagtc ggtagaatcc tgtaaatcac
    66961 ttgcagaata cagcttttca aaagcttttg caaatgcttc tttgacttct tttacaaaaa
    67021 ggggttggtc aagcagttct tcaagtgttg ccattgtagc aggatccact gcaggatagc
    67081 catttgtacc aaagtcttct tttaaagcag attgataaaa tcgcttcatg acttgcccgc
    67141 gtttttcctg attaccgttg acacttaagt acatcataac agaaatccca tttttgatcc
    67201 gacgctgagc gataccggca aattttttgc catggatgct taaatcataa gttccaggac
    67261 aataagaatg ggtgatttcg aatgcttgta tcggtgcctt tggaaaagcg tatttagtca
    67321 ataggagcat ttgctcgtaa gcgtcatcta tggttatttt ttttgtgtca ctttggggga
    67381 aaatcaaaga aatattcaaa attccttcat ctgatacgat ccctaatcca cctgaatttc
    67441 tgatcactgg agcgtaacct gcatctttca gtgtttggat cccgtcactt agaaaaggag
    67501 ttcgaacatc cttcatacct aagatgactg tgcagtcata ttgccagaaa tgtaaaaaga
    67561 tttgctggtg ttctttagaa tattccgtta atatatctgt taatgcaaaa ggggaaaagt
    67621 cgtttttttg ataaatgtgc tgatctagtc gaatcgcagg gttcattcaa ccggctcctt
    67681 tgtataaatt catattttct acaatattat actctaaaat caaagagttg ttttggaaaa
    67741 aaggttttct gtaaaaatgg acaagttggt tgacaaaaca aaagaaacta ggaacaatgt
    67801 agctatgaga agtaaaggag aatgaataat ggaattatca caaggaacac cgatttcaat
    67861 caaattgcgt acaaaagtaa aacagcaagg ggaagtgcaa gatttttatt ttgatttgaa
    67921 agggcaaatg gtgacaattg gcgatacgct gtacatacgt tataaagaga ttcaggagga
    67981 gacgggcgat gagattcctg taacgatcaa attagttcca gatggtcatg tacagctgat
    68041 ccgtgcagga gaaatgcgga tgcgtttgaa atttggctat aaagaacggt tagaaacatc
    68101 ctatcgaaca ccatacggta tgatccagat tgctactttt acgaaagaac tgcatgttag
    68161 tttgaaagac cgtcccacag gagggaaggt tcgaatcgat tatgatctgt tcatgggacc
    68221 ggaaaaaatc ggcgaatatt atttgacctt ggattttaca gcgtaaatat aaaagaaaag
    68281 tttgattttt tgtgtctgat tttgtatgat aaggagtaga ctgtgaaagg acgtgtcctg
    68341 attggaaatt agtgtatttg aaggtgcaaa caaaagcgaa ttatcaatga tcgaagtagc
    68401 acacgcgatc ttagaacaac gtggcgatgt catggatttt tctgatttag ctaaccagat
    68461 ccaaaactat cttgggaaat cagacagtga aattcgtgat tcactcgcac aattttatac
    68521 tgacttgaat attgacggca gctttatctc attaggggat aatcgttggg gcttgcgttc
    68581 atggtatgct atcgattcga tcgatgaaga agtaaaccat ggaatggacg aagaggatga
    68641 agatactcca cgtcgccgta aacgcaagaa agtcaacgcc tttatcaatg atgatgaaga
    68701 cgcgatcgat tacaatgacg atgacccaga agatacagat ctgacagaag aagatgacga
    68761 tgatctgttt gatgacgatg atgatgaaga tgaagaaatc gctgcttata attctgactt
    68821 gcaagaaatc ggagcagatg acccgacaga tgatgaagaa gacttgccag gaaatatcga
    68881 agaagattta agtatcatag acgaagacga tgatgatgaa gattatgaag aggaagaatt
    68941 ttctgactct gaaaaagaat aaacattatt ttcttacgag aggttgtgac agagacgatc
    69001 agcctatcag aataaggagg tgtgacacga tttttgtcac accttctttt ttgtatataa
    69061 aaatcccaag taaatacata aaaaataaaa aaattgaaac ttttagcgca ccttgtacgt
    69121 catattaagt gaggcagaaa ggagtggaga atgattttta ctggatataa gaaaaaacgg
    69181 cagatcgaaa aataccgtca cctgctgaat ggactcatcg aaactgatta tcaaaaaatg
    69241 ttccgtatca cgatgaatta cgtacatgat aaagaagagg ccttagatgt gatgcaagat
    69301 agttttcata aagccctgaa ctattttgaa aaagaaaaag agatcgagca ttttagtgca
    69361 tggttttatc ggatattgat ccgcacggca ttagatagtt ggcgcagaca aaaaaggacg
    69421 cctgtggact tatttgaaat tcaagatatc ccccaacagt tacctttaga aacttccgtt
    69481 gcagagctcc atatgatttt agataaaata gacagtccgg aaaaagagat tctgatcctc
    69541 tacttttttg aaggatttcg tttgaaagaa attgcgacta tcttggatat gaatgaaaac
    69601 acagttaaaa cgaaaatgta ccgctcattg aatgctttac gtcaaatcct agaatgaagg
    69661 agagaagagt gccatgtatt cccaaaaaga aaatttaaaa aaactaaaga aaacttataa
    69721 agaacaagca attcccaaag ggcttaaaga ggagatccgc cagcgtttta ttatcgaaga
    69781 gcaatcattt ataaaagcaa agcggagaaa gagacgtctc caagggttcg gagtatcttt
    69841 ggctatcgta atgattacaa caagtagcct tctttttaat agtcaagttc gaagctttgc
    69901 agaagattta cccatcttag gatcagtcat tgaattgatc ttaggcgaac gctttactga
    69961 tcgatctgaa aaaatcgata ttcaggtacc gaagatcaat acacaaaatg agaaagaaaa
    70021 taaaacaatt catggattaa atcaaaaata ctttcgcgaa ggacaagcag attttgaaaa
    70081 agctgaaaaa gaatatggaa attttgaaac ggatcattac caagttttgg gagattatca
    70141 gaaaatcgta gacgataacc ggtttctcgt gattgaaagg cagatcaccc aaacagcagc
    70201 agatagccat gttgaaaaaa gatacgacac aatcgacaag aaaaacagtg tccagctctc
    70261 acttccactt ttattcaaag atgacaccta tctatctgtt ttgacaaaag aagtcaaacg
    70321 gcagatggcc gaacaagtca aggaagatcc atctaagtac tattggactg agcaagatat
    70381 tcaagaaggg acgatagaaa aaccaacact tgttacgcct acacgaagtt tttatttgaa
    70441 caaagagcat caattagttt tgactttttc tcaatacgaa attgctcctg gttatatggg
    70501 tacaccagaa tttgtcattc ctaagtccgt gacaaaaacg attttggcat cggaagatta
    70561 tttggatcat tagacgagaa gtagttgcga ctagcagttt caattttcaa gtaagtacaa
    70621 gtaaataata agaagagaaa aagagaaaga agagatatag tgaaaagaaa ttatttagtt
    70681 atcagtatta ttgctttatc cgtgcttttg tttttcgaaa ttacttcggt ggcctccttg
    70741 atcaccgaag taaaaggaca aacacactct tcggagcatt cgtcacaatc tttcaaacaa
    70801 tcagaacaat caaccattaa aagccaatca actgaaacag ctaacgtcaa agaagccatt
    70861 caagaatcag cggaaaaggt acaaagtcag tcatcacaat caacacaaga cagtacaaaa
    70921 acgagccgag atccggctgt caaaagcgtc gcaatcagct ttgatgatgg accaggagcg
    70981 accacaacac cgcagcttct gcgaatctta aaagaaaaaa acgtgcatgc cacttttttc
    71041 gtcttaggag aaaatacggc gcagcatcct gaaatcgtga agcagacagc tgaagcaggg
    71101 cacgagattg gcaattacac atacgatcat cagaatctgg ccactctttc tgcccagtcg
    71161 atgacggagg aagtgacgaa agcagacact gagatcaaaa aagtcactgg caaaaccccc
    71221 acttttgttc gtccgcccta tggttctgtc acgaatgtag gagcaacgat catccagcga
    71281 ccgatcatcg aatggtctgt tgattcagaa gattggaaga caagaaatcc tgatttgatt
    71341 ttgcagaaga tccaagcaac agtatacgat ggggcaatta ttttattcca tgatatctat
    71401 cctgaaacga ttcgtgccgt tcctcaagtg atcgattatc tgaaagagca agggtatcgc
    71461 atcacgacag ttggggattt attagggcat ccaacggctg tcgagaacta ttatggcaga
    71521 aacgatcatc ggccagttca gtaagtgaga caagtatctt taataaataa cacattttaa
    71581 taagtagttg aacacataac taccttactt gcaaaggctt aacaatatct atacaggtac
    71641 aacaaaaaac tgtccaccaa ttttctttgg cggacagttt tcacttaaaa ataaagaagc
    71701 aactattcgt aaattatgct aaaatagcag tagaacgcgt cctgagggaa tcgaaccccc
    71761 gtctcaagaa ccggaatctt acgtgatatc cactacacta aggacgcaag cactgggata
    71821 attgtaacta aacagataaa aaattacaag gggattggac aaaaaaatga aatttgagca
    71881 aaatttttca caaaaacagc agcagacaca gaaactagcc atgacgcaac agttgcaaca
    71941 gtccatccaa gttttacaat ataacagtga ggagctgttg gcattcgttg agaatcaagc
    72001 aatggaaaat ccattggtgg aagttgttga gccggaatgg cagccagatt acataaaagc
    72061 ttcttcatct tcttatgaag gagaagagac aaattaccta aatcagattc ctgatacaaa
    72121 agggtcttta tttgattcat tgatcgaaca agtccatttg aattaccgag atactttttt
    72181 gaggaaaatc gttctttatc ttgtagaata catcgatttg aacggatttt tgacgattag
    72241 tttagaagaa gccggaaaag agattggagc tacgcctatc caaatgctcg atgcgttgac
    72301 tctgattcag caattagaac cagctggagt aggtgcacgt agtttgcaag aatgtctcat
    72361 gcttcaaaca gaacgagatg attatgcgcc ggaattagct tatatcgtgt tggaagaatg
    72421 ttttgaggaa ctggtggagc gaaaatggaa agaaatcgca caacgttttt ctgttgatct
    72481 acatgctgtt cagcagatat tcgactattt acaaacgttg agtccaagcc ctggccggat
    72541 tttcgaccgt agcagcgaat tgtacattat tcctgatgtc cgggtattag tggacgacga
    72601 taaaaatgta caagttatct caaaccgaaa aaatcagcca aatatacgct ttcaagagag
    72661 ttacttcaaa caaatgtcac aacaagcaga taaagaaact gaaaattatt taaaagaacg
    72721 gaaacaggaa ttcgaatggt tgaagaaaac gatcctacaa cgtggggata caattttgcg
    72781 ggttgctcaa gtcattgttt cccggcaaaa agctttcttt atcgacaagg aacgtccaat
    72841 caagcctctt acgttaaagg aagttgcaac tgagatagat gtgcatgaat ccacggtcag
    72901 tcgtgcagtc aacgggaaat atttggaaac agatttcggt gtatttgaat taaaaaaatt
    72961 tttcactaca cgaattccta cgaatagtac agaacagaca gaagaccttt cagcagatac
    73021 agcgaagaaa aagctgcagg aattagtcga tcaagaagac aaaaacaagc cgttgtcgga
    73081 ccagaaattg gttgaattgc tgaaaaaaga tgagatagcg atctcaagac gaacagtagc
    73141 aaaatatcgt gatttattag gaatcccgag ttcatcaaaa agaaaacgtt atgataacta
    73201 atcagtcaaa agactagaaa aagtatttca ctttttctag tctttttagt tgaaaaacga
    73261 gagcgaaact gttagactta cagtcgtgag gttgagattt ccctttattg atgtgaaatt
    73321 cgaatagtta atttcttttt ttatcgtata tgggtcgttt taagtctaca ttggacgtaa
    73381 agtgtccaac taaggagatc gctatgctgg atgaaataaa aatgattgaa tctgttgcac
    73441 cagatattat cgaagtgttg caagaacgct ataaaatttt acgaaatatc tattggatgc
    73501 agccgattgg gcgtcgaagt ttatcggaaa gtatggggat cacagaacga gtcttacgaa
    73561 cagaaacaga ccttttgaaa aatctgaatt tgatcgacac ctcaaaaagc ggtatgacgc
    73621 tgacaactaa gggcgaagaa gtttatcaaa gccttgaaaa cttcatggat cagcttttag
    73681 gtacgcatca aaccgaacaa cagttggcag aatattttgg taaccaacgc tgtatcgttg
    73741 tttcaggaaa cagcgaagag cagaccaaag tggctgatgc attcggcgaa gcgctgagcg
    73801 aagccttaga ccgactactt ccagaaggcg agaatatcat cgctgtgatg ggaggaacta
    73861 ctatggctgt ggtagccgaa caattgtcta atctggaaaa taagaaacgg cataacctgt
    73921 ttgtccctgc aagaggcggg attggtgaag cgataactgt acaggcaaat tctgtcagtg
    73981 caagaatggc tgctaaagca aacggtaacc accgcgcact ttatgttccg gaacaattaa
    74041 gtctggcaac ttacaattct ttactcaatg agccatctat ccaagaagtc ttaaacttga
    74101 ttagtgaagc gaattgtgtt attcatagta ttgggcgtgc attacacatg gcagcacgtc
    74161 gtaagatgac tgaaaaagaa ttagtcatgc tgaaacaagc aaatgctgtt gcggaatctt
    74221 ttggctactt tttcaatgaa aaaggtgaag tcgtttataa ggtccctaga atcggcatag
    74281 aattgcaaga tttggaaaaa gtcccgatca tcatggcaat cgcgggcgga aaatcaaaag
    74341 ctaaagcaat tcgagcttat atgaaaaatg caccaaaaca aacgtggctc atcactgatg
    74401 aagctgctgc aaatgagatt ttaaaagggg taacccttta aaataaatat ttttttgatt
    74461 ttcataagga ggaaatctta aatgacagtt aaagtaggta ttaatggttt tggacgtatt
    74521 ggacgtctag cattccgtcg tatccaagat gtagatggaa tcgaagtagt agcaatcaac
    74581 gacttgacag atgctaaaat gttggcacac ttgttaaaat acgacacaac tcaaggacgt
    74641 ttcaacggaa cagttgaagt tcatgaaggt tcattcaacg taaacggaaa agaagttaaa
    74701 gtattagcta accgcaaccc agaagaatta ccatggggcg aactaggcgt agatatcgtt
    74761 ctagaatgta ctggtttctt cacttctaaa tcagcagctg aaaaacattt aacagctggt
    74821 gctaaacgcg ttgttatctc tgctcctggc ggaaacgatg ttccaacaat cgtttataac
    74881 acaaaccacg aaacattaac tggtaaagaa acagttatct caggtgcttc atgtactact
    74941 aactgtttag ctcctatggc taaaacattg aacgacaaat ttggtgttgt tgaaggatta
    75001 atgacaacta tccacgctta cacaggtgac caaatgactc tagacggacc tcatcctaaa
    75061 ggtgacttcc gccgtgcacg cgctgctgct gcaaacatcg ttcctaactc aacaggtgct
    75121 gctaaagcta tcggtttagt aattcccgaa ttgaacggta aattagacgg agctgctcaa
    75181 cgtgttcctg taccaacagg ttcattaaca gaattagtaa cagttcttga aaaagaagta
    75241 actgttgacg aaatcaatgc agcaatgaaa gaagcttcaa acgaatctta cggatacaac
    75301 acagacgaaa tcgtttcttc agatatcgtt ggtatgactt acggttcatt atttgatgct
    75361 acacaaacta aagtaatgac agttggcgac aaacaattag ttaaaactgt tgcttggtac
    75421 gacaacgaaa tgtcatacac tgcacaatta gtacgtactt tagaatactt cgctaactta
    75481 taagatagac aaacgtacaa gtgtaaatta tagacatgta catttaaaag cggggaagca
    75541 atcgcgcttc ttccgctttt tttgataata agtttattca ggaggaaata aaatggctaa
    75601 gaaaacagtg aaagatatcg atttaaaaga caaaaaagtt cttgtccgtg ttgacttcaa
    75661 cgttcctttg aaagacggcg tgatcactga cgacactcgt atcaaagcag cattgccaac
    75721 aatcaactac gttctagaac aaggcggaaa agcaatcctt ttctctcacc ttggacgtgt
    75781 aaaaacagaa gaagataaag aaggaaaatc tttagctcca gttgcaaaac gtctaggcga
    75841 attattaggt aaagaagtaa cattcgtacc tgaaactcgc ggagaacaat tagaagaagc
    75901 aatccgcaac atgaatgacg gcgacgttgt cgtattcgaa aatacacgat ttgaagatat
    75961 tgacggtaaa aaagaaagca aaaatgatac agaactaggt aaatactggg cttcattagg
    76021 cgatgtattt gttaacgatg ctttcggtac agctcaccgt gcacatgctt ctaacgtagg
    76081 tatcgcttca actggtatcc caacagttgc tggattcttg atggaaaaag aaatcaagtt
    76141 catcggtgaa gcagtagaag aaccaaaacg accaatgatt gcaatccttg gtggcgcaaa
    76201 agtttctgat aaaatcggcg taatcgaaaa cttgattcct aaagcagata aaatcttgat
    76261 cggtggcgga atgacttaca cattctacga agcaaaagga atcaagatcg gtaactctct
    76321 agtagaagct gataaagtgg aactagcaaa agaattaatc gaaaaagcag gcgacaaact
    76381 tgtcttgcca atcgacaatg tctgtgcacc agaattttca aatgatgttg aaacacaagt
    76441 aatcgaagga gacattcctg atggtttgat ggctttagac atcggcccag catcagtaaa
    76501 attatttgct gatacattac aaggtgcaaa aacagttgtt tggaacggac ctatgggtgt
    76561 atttgaaatg agtaactttg ctaaaggtac aatcggtgtt tgtgaagcaa tcgctaactt
    76621 agaaggcgca acaacaatca tcggtggcgg agactcagcc gcagcagcag aacaattagg
    76681 atttgctgac aaattcacac acatctcaac aggtggcggc gcaagcttgg aattgcttga
    76741 aggtaaagaa ttaccaggac tagcagcaat caacgataaa taaacgacca agtttgctca
    76801 attctgacga gtaatcgaac aaaaggtgaa gggagttttc atgactccct agccattttt
    76861 caataaatta aggagtgtgt tcctcaatgc gtaaacctat tatcgcaggt aactggaaaa
    76921 tgaacaaaac tgcatcagaa gcaaaagaat ttgctgaagc agtaaaaaac aaaatccctt
    76981 caaatgacaa agttgattca gtcatcggat ctcctgcatt attcttacaa gaattaatga
    77041 catcagctga aggtacagaa ctaaaaatct cagcacaaaa ctgctactgg gaaaactcag
    77101 gtgcgttcac tggtgaaact tcaccagcag ctcttgctga tctaggtgtt cattatgtga
    77161 tcatcggaca ttcagaacgt cgtgaatatt tccatgaaac agacgaagac atcaataaaa
    77221 aagcgaaagc tatctttgca aatggtatga caccaatcct atgctgcggc gaatctttag
    77281 aaacctatga agcaggcaaa actgctgaat ggatcgaagg acaaattaca gctggtttag
    77341 ctgatctttc agctgaacag gtgtcaaaca tggttattgc ttatgaacca atctgggcaa
    77401 tcggtactgg taaatcagct gacgcacaaa tcgctgacga aatttgtgga gttgtacgtc
    77461 aaacagttga aaaactttat ggaaaagaag tatcagaagc agttcgtatc caatacggcg
    77521 gttctgtaaa accagaaaat attgctgaat acatggcaaa agaaaatgtt gacggtgctt
    77581 tagtcggtgg cgcaagcttg caagcagatt cattcttgtc attgttagac gctgtaaaat
    77641 aattgttgaa aatttgcgaa aaatgcaaga aatgtttgca ctaatgagca aaattaacta
    77701 aaatcgtata tgagggaaat cccttaggaa aaaacaaact caaaggagag acaaaacatg
    77761 tcaattatta ctgatgttta cgctcgcgag atcttagact cacgtggtaa cccaacaatc
    77821 gaagtagaag tatacacaga aagcggagct tttggccgcg gaatggttcc ttcaggtgct
    77881 tcaactggtg aatacgaagc agttgaatta cgtgacggag acaaagctcg ttatggcggt
    77941 aaaggtgtaa ctaaagctgt cgacaacgta aacaacatca ttgctgaagc aatcattggc
    78001 tacgatgttc gtgaccaaat ggctatcgac aaagctatga tcgctttaga cggaactcct
    78061 aacaaaggta aattaggtgc gaacgctatt ttaggtgttt ctatcgctgt tgcacgtgca
    78121 gctgctgact acctagaagt acctttatac cactacctag gcggattcaa cacaaaagta
    78181 ttgccaacac caatgatgaa catcatcaac ggcggatcac atgctgataa ctcaatcgac
    78241 ttccaagaat tcatgatcat gcctgttggc gctccaacat tcaaagaagc tctacgtatg
    78301 ggtgctgaag tattccacgc attagcttca atcttgaaag ctcgcggatt agcaacttct
    78361 gttggtgacg aaggtggatt tgctccaaac cttggttcaa acgaagaagg ttttgaagta
    78421 atcatcgaag caatcgaaaa agctggctat gtacctggta aagacgttgt tcttgctatg
    78481 gatgctgctt cttcagaatt ctacgacaaa gaaaaaggtg tttacgtact agctgactca
    78541 ggcgaaggcg aaaaaacaac tgacgaaatg atcaaattct acgaagaatt agtttctaaa
    78601 tatccaatca tctcaatcga agatggatta gacgaaaacg actgggacgg attcaagaaa
    78661 ttaactgacg tattaggcga caaagttcaa ttagttggtg acgacttgtt cgtaacaaac
    78721 actcaaaaat tatctgaagg tatcgaaaaa ggaattgcta actcaatcct tatcaaagtg
    78781 aaccaaatcg gtacattaac agaaactttc gaagctatcg aaatggctaa agaagctggc
    78841 tacacagcag ttgtatctca ccgttctggt gaaacagaag attcaacaat ctctgatatc
    78901 gctgttgcaa caaacgctgg ccaaatcaaa actggttctc tatcacgtac tgaccgtatt
    78961 gctaaataca accaattact acgtatcgaa gaccaacttg gtgaagttgc agaatacaaa
    79021 ggtttgaaat ctttctacaa cttaaaaaac aaataattct tttctaatga agatacatga
    79081 tcgactattg aaagatagtc gagtatgatc acttagttaa gtaatcgaac tctctacttg
    79141 ctgaaatatc agcaggtaga gagttttttt gcaactagct tctgtatatg atgtttacaa
    79201 agaatggtac aagaaaagat catccttgaa aaaggaaagc taacaataac tagcatcata
    79261 taagagcttt taaggataga acagtagaat aggaatctat gggcaagtcc tctcgtataa
    79321 ataaaatagg agggaagtag cagcatattt ttcataaagt ggctctcttt cttcattttt
    79381 actttggttg ctcctatttg aaaattcgag cacactcaaa tacaataaaa agaaatggca
    79441 agggagagga ttagatgaaa aaagaagaaa aaatcgcgat tttacaagag attatccgaa
    79501 ttaaatcagt gaatggaaac gaaggtgaag tagcagccta tttgaataag cttttggcaa
    79561 agcatgacat aacaggagag attgtttctt acagagatgg tagagataat ttgattgctc
    79621 gttaccaaaa gggccaatca ggaaaagtgt taggcctatc tggacatatg gatgtcgtgg
    79681 cggctggtga tgaatcgtct tggacctatg caccctttgc agcagaaatc catgggaacc
    79741 gtttgtacgg acgtggtgct acagatatga agagtggctt ggcagcaatg gtgattgcca
    79801 tgatcgaact gaaagagtca ggaaaaccat ttaacggaac agtcaaatta ttagccactg
    79861 tcggagaaga agtgggtgaa ctaggtgggg aacagctcac gaaagcaggc tatgtagatg
    79921 acttagatgc cttgatcatc ggagagccaa ccaattacag tttgatgtat acgcacatgg
    79981 gatcaatcaa ctatacagtc acctcgcacg gaaaagaagc gcatagctct atgccagacc
    80041 aaggttataa tgcaatcaat cacctaaatg aattcattac gaaagcaaat gctgaaatga
    80101 atcacctcgc tgaaacaatc gagaatcctg tcctaggaaa aacaatccat aatgttacat
    80161 tgatcagtgg cgggaatcaa gtcaacagca tcccaagcca tgcgcagctc caagggaaca
    80221 ttcgatcgat tccagaatac ccaaatgata aaatcattgc attgctgcaa tcaatcgtca
    80281 atgaattgaa tcaagagaca gactatcatt tggaattgat gatcgattac aacaaaatac
    80341 ctgtcaaagc tgatccagat tctcctttga ttcattgcat tcagcaacaa ttcagccagc
    80401 cattaccttt agttggagcg gcagcaacaa cggatgcagc cgaatttacg aaagctaacc
    80461 attccttcga ctttgtcgtc tttgggcctg gtgtcgttac tttgcctcat caagtagatg
    80521 agtatgttga aatcgataat tatttggata tgatcgaaaa atatcaagga attattttat
    80581 cctacttagc gtaagatttt tttaataa
//
LOCUS       DAZKLD010000002        69148 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.002, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000002 DAZKLD010000000
VERSION     DAZKLD010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 69148)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 69148)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..69148
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.002"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            87..257
                     /locus_tag="ACG8KR_000082"
     CDS             87..257
                     /locus_tag="ACG8KR_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0199491.1"
                     /translation="MKWKEKVDEAAEKLYDLVKSEKYNIEVNIPKKGGKAVQVKSKRP
                     TNHTKKWMAKNR"
     gene            477..1184
                     /locus_tag="ACG8KR_000083"
     CDS             477..1184
                     /locus_tag="ACG8KR_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFQ0199492.1"
                     /translation="MSNQLPVYIQIHDQLKNEIEQGIWKVGDRLPSERELAVKFNVSR
                     MTLRQAIQNLADEGILERKIGSGTYVAREKVQEKMSGATSFTEIMESQNRVPSSRTIS
                     YFLTSPSSSEMEKLNLSKDDTILRMERIRFADDVPICFEVASVPQKLIDGYSKAEITE
                     SLYRTLEEKGGKKIGPANQTITAVLASEKIADYLEVKRGDAVLRLRQISYFEDGTPFE
                     YVRTQYVGNRFEFYLEK"
     gene            complement(1244..1888)
                     /locus_tag="ACG8KR_000084"
     CDS             complement(1244..1888)
                     /locus_tag="ACG8KR_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HFQ0199493.1"
                     /translation="MIERYRTIKENGQSEIEIKKSRFICSLRRIESEDQAKSFIQEIK
                     KEHWKANHNCSAFIIGEKNEIQRSSDDGEPSGTAGVPMLEVLKKNQLINVAAVVTRYF
                     GGTKLGTGGLIRAYTHAVSQALSDIGIVEGTLQQEISVMITYPQLGKLQNYLELQHFF
                     IKEIQYTDQVSVICAVDEKSADHFKESIIELLNGQVQFEEGPVSYHEQLVTDHI"
     gene            2015..2302
                     /locus_tag="ACG8KR_000085"
     CDS             2015..2302
                     /locus_tag="ACG8KR_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0199494.1"
                     /translation="MQERMKKYDAITHYLKNNGGSQVTLTFTQFDELLFPSNGLPKTA
                     RESTDWWANDYKHPEKGAYGWINAGYEVVVINLDKEYVVFNKLVKSSWLFD"
     gene            2410..2730
                     /locus_tag="ACG8KR_000086"
     CDS             2410..2730
                     /locus_tag="ACG8KR_000086"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFQ0199495.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEAKGIDSDIFAVSASDA
                     DNNLANKDVDVLLLGPQVRFMKADFEKRLEPKGIPLDVINMADYGMMNGEKVLDQAIT
                     LIGE"
     gene            2811..4124
                     /locus_tag="ACG8KR_000087"
     CDS             2811..4124
                     /locus_tag="ACG8KR_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HFQ0199496.1"
                     /translation="MNHFWGRRLLTRDIKAIDCEEVNLPNYEKIAAVERLGNWILCHR
                     CGMRTSVIDGQLADYGYFCTHCLYLGRCDTQQDLYLFDQPEAKPREVVFFWTGQLTEK
                     QTEIAKRILCHSEKRHHLIWAVTGAGKTEMLYPILVETLKAGGRVAICTPRVDVCNEL
                     FLRYRYVFPEEKITLLHGNTATAYTFTSFVICTIHQLYRFYRSFDLIVIDEIDAFPYN
                     GDAGLAHAVQTAIKPDGRFIYLSATPDKKLLKEIKQTFDIHKLALRFHRRLLPEPVLV
                     FMNNWRQKCKNKKKIKSLVRIIHQLIKENHILLFCPSITMMHRLKATLQEALPRYEIT
                     DVSSRDNQRAEKVQKMREAKYDILLTTMILERGVTFERISVIVLGADHPVFTKSSLVQ
                     IAGRADRKGPYTNSQVYFFYEEKTAAIRKACQEIKEMNEEGKKML"
     gene            4121..4840
                     /locus_tag="ACG8KR_000088"
     CDS             4121..4840
                     /locus_tag="ACG8KR_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HFQ0199497.1"
                     /translation="MRCSCCSKDIVQNLTFSEIIGVSKYRLPQTCSVCRAQFHLLETR
                     GCSGCQKPIHFNLESTKKKVKSIPVYCSDCQEWKKKYPTYDFQHRAFFVYDENMKAWL
                     NQYKFMGDIRLAGTFAYKWTQIKKEYPTYIYCPIPLSEKRRTERGFNQVSEMLKAARI
                     PFQMLLKREKHLLAQAKNTRKERLEMPQPFELAVTFSKIQNKKILIIDDVYTTGQTLF
                     HAADCLQVAQPKIIRTISLAR"
     gene            4968..5519
                     /gene="raiA"
                     /locus_tag="ACG8KR_000089"
     CDS             4968..5519
                     /gene="raiA"
                     /locus_tag="ACG8KR_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HFQ0199498.1"
                     /translation="MFRYNVRGENIEVTEAIRDYVEKKVGKLERYFSDAPDATAHVNL
                     KVYTEKTAKVEVTIPLPYLVLRAEETSPDLYASIDLVVDKLERQIRKFKTKINRKSRE
                     TGMNTADATALFNEEDENENDSELDIVRTKRLSLKPMDSEEAVLQMNMLGHNFFIFED
                     AETNGTSIVYRRKDGKYGLIETD"
     gene            5741..8275
                     /gene="secA"
                     /locus_tag="ACG8KR_000090"
     CDS             5741..8275
                     /gene="secA"
                     /locus_tag="ACG8KR_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HFQ0199499.1"
                     /translation="MANLIRSLIENDKKELKRLDSIAKKVEAYADQMAALTDEQLQAK
                     TAEFKGRYQKGETLDQLLPEAFAVVREAAKRVLGLYPYHVQLMGGIVLHDGNIPEMRT
                     GEGKTLTATMPVYLNALTGEGVHVVTVNEYLATRDSTEMGELYNFLGLTVGLNINSKS
                     SDEKREAYNCDITYSTNNELGFDYLRDNMVVYRHQMVQRPLNYAIVDEVDSILIDEAR
                     TPLIISGQAEKSTALYTRADNFVKRLKEEEDYKIDIQSKTISLTEAGIEKAEETFGLD
                     NLYDIENTALTHHLDQALRANYIMLLDIDYVVQDGKVLIVDQFTGRIMDGRRYSDGLH
                     QAIEAKEGVEIEDETKTMATITFQNYFRMYKKLAGMTGTAKTEEEEFREIYNIQVYQI
                     PTNRPVIRDDRPDLLYPTLQSKFHAVVQDIKDRYRKGQPVLVGTVAVETSELLSDMLN
                     QERIPHEVLNAKNHFKEAEIIMNAGQKGAVTIATNMAGRGTDIKLGLGVRELGGLAVI
                     GTERHESRRIDNQLRGRAGRQGDPGMSQFYLSLEDDLMKRFGSERIKAFLDRMKIGDE
                     DAVIQSKMLTKQVEAAQKRVEGNNYDTRKNVLQYDDVMREQREVIYKQRQEVIMEEKS
                     LSKPLMNMVKRTISRVVDAHTQLEKENWNLESIVDFAGSTLVHEDSISLTDIEGKTSQ
                     EIKEYLNERAQEVFNTKAAQLNGPEQLLEFEKVVILRVVDSKWTDHIDAMDQLRQSIG
                     LRAYGQNNPLVEYQTEGYKMFEDMVGAIEYEVTRIFMKAEIRQNVQREQVSQGTASHS
                     EDGDAKENSNTSAKKQPIHVNKVGRNDPCPCGSGKKYKNCHGKDAN"
     gene            8402..9503
                     /gene="prfB"
                     /locus_tag="ACG8KR_000091"
     CDS             join(8402..8473,8475..9503)
                     /gene="prfB"
                     /locus_tag="ACG8KR_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_139124933.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HFQ0199500.1"
                     /translation="MEISEIRNLLTEMQEKVTSFRRSLDLDQLEEDIAEAEAKMAEPG
                     FWDDSEAAQKVISENNANKETYDQFHSLAEELEEIELMVEMLQEEPDAVMQEEAEARI
                     NALDEKMNTYELSMLLDGPYDRNNAIVELHPGAGGTESQDWGSMLLRMYTRWAEQHGY
                     QVETLDYQAGDEAGIKSVTLLIKGHNAFGYLKSEKGVHRLVRISPFDSAKRRHTSFCS
                     VDVMPELDETIDIEINPDDLKIDTYRASGAGGQHINKTESAVRITHLPTGTVVASQAQ
                     RSQLKNREQAMGMLKAKLYQLEVEKKEQEAAALRGEQKEIGWGSQIRSYVFHPYSMVK
                     DHRTNVETGNVQAVMDGDLDLFIDGYLKMHLQ"
     gene            9608..10294
                     /gene="ftsE"
                     /locus_tag="ACG8KR_000092"
     CDS             9608..10294
                     /gene="ftsE"
                     /locus_tag="ACG8KR_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HFQ0199501.1"
                     /translation="MIEMKDVMKKYSNGTTAIRNLSIVIEQGEFVYVVGPSGAGKSTF
                     IKLMYREEKASKGTLNVAGHDLIAIKNREVPYLRREIGVVFQDYKLLPRKTVYENVAY
                     AMQVIGRKPRDIKKRVMEVLDLVGLKHKVRVFPSELSGGEQQRVSIARAIVNTPKVLI
                     ADEPTGNLDPENSWEIMKLLDRINAQGTTVVMATHNSTIVNTIRHRVIAIENGRIIRD
                     QAEGEYGYDD"
     gene            10287..11171
                     /gene="ftsX"
                     /locus_tag="ACG8KR_000093"
     CDS             10287..11171
                     /gene="ftsX"
                     /locus_tag="ACG8KR_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HFQ0199502.1"
                     /translation="MIRTFFRHLLESIKSLKRNGWMTVASVSAVTITLTLVGVFMAVI
                     MNATKLAQDIEGNVDVSVFVDIGTKDEEMKKLEKELNDLDHVKKVEFSSKEQELKKIQ
                     NEMGDSWKLFEGDNNPLYDVYVVSATDPSYTKQVAEKAADLKNVSRSDYGGASSDRIF
                     QIAGAVRTWGLVAAALLLFVAMFLISNTIRITILSRQREIQIMRLVGAKNGYIRWPFF
                     LEGGWIGLIGAILPILIITFGYAWFYQLINPSLLRSHYSLIHPENIVWKINLLMVGIG
                     VIIGSLGSIISMRRFLKF"
     gene            11323..12771
                     /gene="cls"
                     /locus_tag="ACG8KR_000094"
     CDS             11323..12771
                     /gene="cls"
                     /locus_tag="ACG8KR_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HFQ0199503.1"
                     /translation="MKIIVDNFFTILLIMNILLSFIIVFRERKETAQTWAWLLVLMFI
                     PVVGFILYIFLGRGISKEKIFDLKIQGKIGKNLEIEEDKQALMRGLYPHPPTGQVDVK
                     QLIYMLTVFESTLYTTKNEITLFTDGREKFDALIEDIKQAEDHIHFQYYIYRSDTLGE
                     EVREALTEAARRGVKVRVLLDAWGSTQVSSSFFQNLKKAGGEVAFFFPLFVPYINPRI
                     NYRNHRKIVVIDGKIGYTGGFNVGNEYLGLVKKFGYWRDNHLRIYGEAVYTLQNRFLM
                     DWNSQHTKEVGYSPEFFPTIHSTGDMAVQIVTSGPDTEHEQIKMTYLKMISLAKREIL
                     IQTPYYIPDGSIHEALKLALLSGVKVHIQIPNKPDHLLVYWATYSFAAELIEYGARIE
                     TYENGFIHAKTMIIDGGIVSVGSANIDVRSFRLDFEVNTLIYDERMASRVRKAFFEDS
                     KISTHLTKEVYENRGMMIKMKEGLARLISPLL"
     gene            13108..13965
                     /locus_tag="ACG8KR_000095"
     CDS             13108..13965
                     /locus_tag="ACG8KR_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HFQ0199504.1"
                     /translation="MKKLKLFLPIIGLAILLSGCSKWIDRGESITAVGSSALQPLVET
                     VAETYQAENPGKFINVQGGGSGTGLSQVQSGAVDIGNSDLFAEEKSGIDASALVDHRV
                     AVVGITPIVNKDIGVKDISMENLKKLFTGEIKNWSEVGGKDQPVVILNRASGSGTRST
                     FEKWVLDGETAVQAQEQDSSGMVRQIVADTPGAISYVAFSYVSDDVDTLNVNGVEPTD
                     ENVTTNKWTIWAYEHMYTRDHPKKLTKEFLDYILSDEVQDNIVGELGYIPVSHMKVER
                     DWQGNIVKE"
     gene            14080..15000
                     /gene="pstC"
                     /locus_tag="ACG8KR_000096"
     CDS             14080..15000
                     /gene="pstC"
                     /locus_tag="ACG8KR_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HFQ0199505.1"
                     /translation="MEDVKKVLLTKSKRSKMEQRGKFISFLCIALIVLVVLSIFYFVA
                     SKGLATFFTDKINVFDFLFGTDWNPSQIGANGKPMVGALPMITGSFIVTFLSAIVATP
                     FAIGAAVFMTEISPKNGTKILQPVIELLVGIPSVVYGFIGLSVIVPFVRSIFGGTGFG
                     ILAGTFVLFVMILPTVTTMTVDALKAVPRHYREASLALGATRWQTIYKVVLRAAVPGI
                     LTAVVFGMARAFGEALAIQMVIGNAALMPTGLTTPASTLTSILTMGIGNTIMGTVENN
                     VLWSLALILLLMSLFFNIVIRMIGKKGELK"
     gene            15000..15884
                     /gene="pstA"
                     /locus_tag="ACG8KR_000097"
     CDS             15000..15884
                     /gene="pstA"
                     /locus_tag="ACG8KR_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010764435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HFQ0199506.1"
                     /translation="MNAKRSDKIATGVLYVVSGIIVLILAALLLYILVRGIPHISWSF
                     LTEPSKAYQEGGGIGIQLFNSIYLLLITMIISFPISLGAGIYLSEYAGKNWLTDVIRT
                     SIEILSSLPSVVVGLFGFLVFVIQFQYGFSIISGALALTVFNLPLLTRTVEESLQAVH
                     YTQREAGLALGLSRWETVIKVVVPEATPGILTGVILSSGRIFGEAAALIYTAGQSAPA
                     LDFTNWNPLSVSSPISIFRQAETLAVHIWKINTEGTMPDGVSVSAGASAVLIIAVLLF
                     NFGARAIGKRLYRKMTSA"
     gene            15899..16705
                     /gene="pstB"
                     /locus_tag="ACG8KR_000098"
     CDS             15899..16705
                     /gene="pstB"
                     /locus_tag="ACG8KR_000098"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002408906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HFQ0199507.1"
                     /translation="MKKYNWNETNVITMPKDKEVALYTNDLHVWYGDNEAIKGVDLEF
                     EKNKITALIGPSGCGKSTYLRSLNRMNDGIANTKITGKIMYKDVDINAPQVDVYEMRK
                     RIGMVFQRPNPFSKSIYDNITFALKQHGEKNKKKLDEIVETSLKQAALWDQVKDSLDK
                     SALALSGGQQQRLCIARAIAMKPDILLLDEPASALDPISTGTVEETLVNLKEDYTIII
                     VTHNMQQAARISDYTAFFYLGKVMEYDKTNKIFTRPKIQATEDYVSGHFG"
     gene            16721..17479
                     /gene="pstB"
                     /locus_tag="ACG8KR_000099"
     CDS             16721..17479
                     /gene="pstB"
                     /locus_tag="ACG8KR_000099"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HFQ0199508.1"
                     /translation="MTKEIISSKDLHLYYGKKEALKGIDLSFNQGEITAMIGPSGCGK
                     STYLRSLNRMNDLIPNVTITGSVLYKDKDIYGPKTDVVELRKEIGMVFQQPNPFPFSI
                     YENVIYGLKLKGEKDKKVLDQVVEESLKAASVWEDVKDKLHKSALSLSGGQQQRVCIA
                     RVLAVNPEIILLDEPTSALDPVSTGKIESMLLELRERYTMIIVTHNMSQASRISDKTA
                     FFLDGHLIEYNDTKKIFMNPEKQETEDYISGRFG"
     gene            17492..18169
                     /gene="phoU"
                     /locus_tag="ACG8KR_000100"
     CDS             17492..18169
                     /gene="phoU"
                     /locus_tag="ACG8KR_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0030643 - intracellular phosphate ion
                     homeostasis [Evidence IEA];
                     GO_process: GO:0045936 - negative regulation of phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HFQ0199509.1"
                     /translation="MLRTQFEEELLNLHNQFYEMGMMVSSAVHKSVRAYIKHDKILAQ
                     EVIENDININNMETRLEKKSFEMIALQQPVTTDLRMIITVMKASSDLERMADHAVSVA
                     KSTIRVKGQTRIPEIEKEISDMSDYVKKMVDNVLVAYVKTDEKDARTIANMDQRVNDY
                     FNRIYNATIKNMQENPETVISGTDYLNVASYLERIGDYVTNICEWIVYLATGKITELN
                     TNHNEIF"
     gene            18733..19425
                     /locus_tag="ACG8KR_000101"
     CDS             18733..19425
                     /locus_tag="ACG8KR_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HFQ0199510.1"
                     /translation="MLLIGGKQGVFALGSLIINIVLLLLLLLLFTKTEGISLLNIAIL
                     FVVIGIIVSILALDGWNRSSIIKISAAVTSVFLAFFICLLTMNHWKDQGLRYEELNYL
                     TRPYRSVFLSSVLIGTAGGTLDTVITVIATLEELTKQQPKISAKQLWLSGRQVGRDVI
                     GSMANILLFVYISGAIPSLLLYLGNQWSFKETVERHLSLELLRVIAGSLGIVLSIPIA
                     VLFFLAVRRLKK"
     gene            19422..20189
                     /locus_tag="ACG8KR_000102"
     CDS             19422..20189
                     /locus_tag="ACG8KR_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HFQ0199511.1"
                     /translation="MNVLIILVGIFAISVLFVGGTQGMYILLGLFINLGIFFLLLFGY
                     HQKWPILVLSIIGFLLIAVVILFFINGYNLKMRAAFASILIFLFCFLLLIPITDFLAI
                     QGFTSIELEELSGLDKTLAIDFRLLTRSLLLISLSGAVLDASVAISSGTFEVYQANPQ
                     MSFNQLRHASFAIAKKILASTVMTLLFAFMGSSLALILWFIDLDISFQQIINEKSFVL
                     EYTMAILTTLAALLVLPLTCVTTAYLFTKKKEHLKME"
     gene            20289..21857
                     /gene="liaX"
                     /locus_tag="ACG8KR_000103"
     CDS             20289..21857
                     /gene="liaX"
                     /locus_tag="ACG8KR_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="daptomycin-sensing surface protein LiaX"
                     /protein_id="HFQ0199512.1"
                     /translation="MKERERILDLVKKGILSTEEGLDLLESMATEKDEKQIKKEADKV
                     TASHKEKDQASQLIDELENGEEKISEPVDPKEKERQDQENLEKILDELATEANKTSAR
                     LDEVNAQFADNKAARNEKQEALMQLNTKEELGELTEEELAQRQTLEAEIKELEATGDT
                     LLEEQLKLEAELKDIRKNQWSEKKETFTDKFELPDDWKDQATDTLNQVGEKMSEAGSQ
                     LGKFLKKTFQTVSETVNDNMEWKDVSLRVPGIATTKFEHEFYYEAPAASILDIKAANG
                     NVTLKTWDSDDVKVEAKIKLYGKMGAEPFEAFSERSQIEVNEDHISFQIPNKRVRADL
                     VFYLPKRVYDHAAIKLLNGNIMIETLEAKDIYTKSTNGNIIVNQLTATMLEVEGVNGN
                     IDIRNGNILDSIIETVNGTVTFGATPENLSVSLVNGDVRLTIKEDNLKKVEASSVNGN
                     VKVALPDSIGLEGHAKTSLGSINSRLSNYEVVREKKERTNQMLQFRRVSDDEIAQVQL
                     STTTGSIYLKDTDK"
     gene            22033..22341
                     /locus_tag="ACG8KR_000104"
     CDS             22033..22341
                     /locus_tag="ACG8KR_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HFQ0199513.1"
                     /translation="MMKKKLTKSNKNVVLTGTLAGIGEYIGIDPTVIRVLYVFLSLVA
                     FGSPIILYILLALIIPSARSARTNYGHNNAYYEKNNYREQTKKRKEAEKIDDDEWSDF
                     "
     gene            22341..22691
                     /locus_tag="ACG8KR_000105"
     CDS             22341..22691
                     /locus_tag="ACG8KR_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HFQ0199514.1"
                     /translation="MSYFQRILVNTLTFISLAVLFPAMVHVSSIWTAVLASFVLSVLN
                     MFVKPILTILSLPFTLVTLGLFTFVINAAILKMTSFFVGAEHFSFSSFGAALFVAILM
                     SLVNVIVSEHNSRK"
     gene            22857..23795
                     /gene="hprK"
                     /locus_tag="ACG8KR_000106"
     CDS             22857..23795
                     /gene="hprK"
                     /locus_tag="ACG8KR_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006109 - regulation of carbohydrate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HFQ0199515.1"
                     /translation="MSEVVKVSQLVKKLSLEIVTGDEKSLDRVIVTGDISRPGLELTG
                     YFNYYSHNRLQLFGSKEISFAERMIPEERLIIMRRLCSEDMPAFIISRGLPAPEEMIQ
                     AANENGIAVLRSPISTSRLLGELSSYLDGRLAPRTSVHGVLVDVYGLGVLIQGDSGIG
                     KSETALELIKRGHRLIADDRVDVYQQDELTVIGEPPKILEHLIEIRGIGIIDVMNLFG
                     ASAVRGSMQVQLAVYLEAWAKDKKYDRLGSEDTTVEIAEVGIPQIKIPVKTGRNVAII
                     IEVAAMNFRARTMGFDATKTFEERLSRLIEENSGND"
     gene            23810..24640
                     /gene="lgt"
                     /locus_tag="ACG8KR_000107"
     CDS             23810..24640
                     /gene="lgt"
                     /locus_tag="ACG8KR_000107"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HFQ0199516.1"
                     /translation="MLAQINRVAFELVGIPIYWYALIIVSGIIIAMWLSSREAVRVGL
                     KADDVTDFMLWGLPISIIGARLYYVLFDLPQYIADPIQIFNIRSGGLAIYGGLIAGAI
                     TLYFFTKYHFISLWTFLDIAAPSVLLAQAIGRWGNFMNHEAYGPDTTRSFLESLHLPQ
                     FIIDNMYIEGIYRQPTFLYESLWSLIGFILLLVLRKKPHLLKKGEVLLCYLMWYSFGR
                     FFIEGLRTDSLYFLGVIRISQLLSAVLFVGTLILFIWRRKKGSLPDYDRSLGKNQQII
                     "
     gene            24684..25709
                     /locus_tag="ACG8KR_000108"
     CDS             24684..25709
                     /locus_tag="ACG8KR_000108"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046168 - glycerol-3-phosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HFQ0199517.1"
                     /translation="MNYRVAVLGPGSWGTALAQVLAENGHDVRIWGHRASQVEEINTQ
                     HTNQRYLPDHHLPDSIRAFTDMEDAVKDADAVLFVIPTKAIRSVAKELVAKLNTKPVI
                     IHASKGLEQGTHKRISEILLEEIPTEKRKAVAVLSGPSHAEEVAKHDITTITAASTNV
                     KAAKYVQELFMNHYFRIYTNPDVIGVEMGAALKNIIAIGAGAIHGLGFGDNAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLVVTCTSVHSRNWRAGNLLGQGQPLEEVLA
                     NMGMVVEGVNTTQAAMELAQMMEIEMPITQTIYEVLYEQKEIKTAAKEIMLRDGKKEN
                     EFSLTIF"
     gene            25724..26662
                     /gene="galU"
                     /locus_tag="ACG8KR_000109"
     CDS             25724..26662
                     /gene="galU"
                     /locus_tag="ACG8KR_000109"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFQ0199518.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILIVTGKAKRPIEDHFDSNIELETNLSEKGKTELLKLVEETTDVNLHFIRQ
                     SHPKGLGHAVLQARAFIGNEPFVVMLGDDIMEDEVPLTQQLIDDYERTHASTIAVMQV
                     PHDETSKYGIINPGEVLEDGLYNVKNFVEKPDPSKAPSDLAIIGRYLLTPEIFDVLSE
                     QQPGAGNEIQLTDAIDTLNKTQRVFARKFTGKRYDVGDKFGFMKTSIEYGLTHPEVGA
                     PLREYIMSLGKTLEAEGSKINHNPKGITEIKEEKKK"
     gene            26787..27236
                     /locus_tag="ACG8KR_000110"
     CDS             26787..27236
                     /locus_tag="ACG8KR_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003126235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HFQ0199519.1"
                     /translation="MSGGEIAGLIAAIAFAILVVFIVRVLLQVSKTLEKVDQTVAEAN
                     TTIEIVTQDVDLLSRQVEGLLVKSNELLLDINGKVATIDPLFTAVADLSESVSELNHS
                     SKNIVTKVGGIGKTTAKATVAGKVGGTAMKFLKTKNILKRQEENKNG"
     gene            27229..27843
                     /locus_tag="ACG8KR_000111"
     CDS             27229..27843
                     /locus_tag="ACG8KR_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HFQ0199520.1"
                     /translation="MAKKGGFLLGAVVGGTAAAIAALLLAPKSGKELRDELAAQADDL
                     MDMASDYTDMAKEKSSELTGIAKEKAQDLSQQAGDLAGNVKQKVSESAEEAGSESDDI
                     LLNLKEQSADLSNRFKKTAEDLKKPADELGSIAKDTSEDIMIDVKDAADKAGETVNEG
                     MSEAKPVADDTKEMLKMAKEDAKEDAKEAKKEMKDTMTDSTTNH"
     gene            complement(27906..29009)
                     /locus_tag="ACG8KR_000112"
     CDS             complement(27906..29009)
                     /locus_tag="ACG8KR_000112"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HFQ0199521.1"
                     /translation="MNNVKINELRQWMAQHQLELAYISDPGHIAYFSGYESDPHERVL
                     ALFIPLEQDPFLFTPALEVEDAQNSSWAYDVRGYLDSENPWEIIGKELRARYGQPGNV
                     GIEKNALTVDRFDAISDILTGTDSYTDLTSIIQRLQLIKTVEEKDKLIEAGKWADVAF
                     EIGFKTVKEGVTEQAIIAEIEYQLKKQGVSQMSFDTLVLAGANAASPHGTPGSTKVSP
                     NELVLFDLGVIWNGYCSDATRTVAYQKPTEFQEKIYNITLEAQLAAQEAVRPGVTAGE
                     LDQIARNVINSYGYGEYFNHRLGHGIGTTVHEFPSLVEGNDLVIEEGMCFSLEPGIYI
                     PEKVGVRIEDCVYVTSDGCVPFTTTPKELLVLE"
     gene            29219..30220
                     /gene="ccpA"
                     /locus_tag="ACG8KR_000113"
     CDS             29219..30220
                     /gene="ccpA"
                     /locus_tag="ACG8KR_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001228 - DNA-binding transcription
                     activator activity, RNA polymerase II-specific [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HFQ0199522.1"
                     /translation="MEKQTITIYDVAREANVSMATVSRVVNGNPNVKPATRKKVLEVI
                     DRLDYRPNAVARGLASKKTTTVGVIIPDVSNMFFASLARGIDDVATMYKYNIILANSD
                     GNDQKEVNVLNNLLAKQVDGVIFMGHHITDEIRGEFSRSKTPVVLAGSIDPDEQVGSV
                     NIDYVSATKDAVNKLAKSGNKKIAFVSGALIDPINGQNRLKGYKEALSENNLPYSEGL
                     IFEAQYNFKDGLSLVDRIHNSGATAVYVSDDELAIGILDGLLDRGVKVPEEFEIITSN
                     NSLLTEVARPRLTSITQPLYDLGAVSMRLLTKMMNKEEVEEKTIILPYGIEEKGSTK"
     gene            30574..30969
                     /locus_tag="ACG8KR_000114"
     CDS             30574..30969
                     /locus_tag="ACG8KR_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0199523.1"
                     /translation="MPKKRRKTIVIILTILTIGIGGKFYMDKREAQKQQDLLAVEKQS
                     VKVLKNTFADIKEVKIEQFEKNDMTGIYEALITMTNTEGKAVYFDYSFTKESNEIDGY
                     GVENEEVQKEGITVDEIIVIYTDGSEEKV"
     gene            30966..32222
                     /locus_tag="ACG8KR_000115"
     CDS             30966..32222
                     /locus_tag="ACG8KR_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipase"
                     /protein_id="HFQ0199524.1"
                     /translation="MTITDKQYKGISNKVYWLDPKHPQYSKSYKEGAIKNFGETKFQI
                     LKIKNSFDGMQAMAVAPIKNGKVDISQVVIAFAGTNLSDWKDLETDARSVMNLYGNRP
                     GASFSEKATTNKILSVSGLKTAGQINSAIAFANEVKKEYGEAAISTTGHSLGGFLALY
                     VAAEKQWKNVGFNGPDPYDLLSPEAKKWVEENPGMLTNYRNHADFIGNFGGNGTGAEV
                     RISMETAYLNLLASHSLTSWKFDENGKLIIPENENNVKARKQQKENQATQLFSYQLQE
                     LNNIRKKLRLSGGRLTVNEQIYLDDTQARLVVEKLSVKMKSAMEEVIRINQRGIFELE
                     KKWQEGLRQARVVAPSLTEQEIIEALASVGATKENLVDSAKEVYLLRINKARRMGEAF
                     DTLAKEIQDKINEIVARDRELAQQFN"
     gene            32234..32557
                     /locus_tag="ACG8KR_000116"
     CDS             32234..32557
                     /locus_tag="ACG8KR_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0199525.1"
                     /translation="MKLEELERDYRSERRQLEAQEDELIHFKNQGLRLMEQEEQRLHH
                     VLKNLALDNEPLFQARRSYSNYQEHYQQALQVERRKLYQQQDELERSYRQKIRQITME
                     KSSYE"
     gene            32550..32816
                     /locus_tag="ACG8KR_000117"
     CDS             32550..32816
                     /locus_tag="ACG8KR_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0199526.1"
                     /translation="MNEFLMQLLKELEKNLHRSNVRSVGMGISQQTWAEKRLTDQNHQ
                     MTAEEATQEFSNRHAHLKESVKGQFAEKLTELFQRQEKKLKQFR"
     gene            complement(32979..35324)
                     /locus_tag="ACG8KR_000118"
     CDS             complement(32979..35324)
                     /locus_tag="ACG8KR_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HFQ0199527.1"
                     /translation="MPRKNTRKKQNRRKKEKWFVPKIIFRVFQSLTVFITVLLLLFAA
                     LGIGIGAGYFAYLVEDTQLPTKKELQTELGNITETSKIVYADNTEISKIQTDLMRTTI
                     SSDKISPLLKTAIISTEDEYFDKHQGYVPKAVLRALVSEATGIGSSGGSTLTQQLVKQ
                     QILTDETTFKRKANEIVLSAQVEKHFSKDEIITTYLNVSPFGRNNKGQNIAGVQEAAR
                     GIFGVDAKDVTLPQAAYIAGLPQSPITYSPYTNTGALKEDLSAGLARKDFVLFSMYRE
                     GQITKEQYEEAKAYDLTKDFLPQQIAEQTDREFLYYTVMNEATRIIAQQLAEKDNADM
                     SDSSVSEAYYQKAQQTIQNKGYTIHSTIDKDIYAALQTGVENYGYMLDDGAGSQVETG
                     NVLMDNRTGRIYGFVGGRNYSQNQNNHAFDTERQAGSSIKPVLVYGPAIDMGLVGSES
                     RVSDYATTWQEGKNAGEKIVNATNEGSNTFQTVRESLEWSNNIPAYHLYQDVLNSGGS
                     KQYAYEHYLAKMNYPANDNWGVESAPLGTVDVTTLQQTNGFQALANGGVYEEGYIIDS
                     ITDNAGNVIYKHESNSVRIYSEATASIMNDMMRSVINEKITTPFKDVISSLNGNLGNA
                     DWVGKTGSTNEYRDSWLIVSTPSITISSWAGHDDNTGMDSQARIRSANYLANLINQAY
                     QANPEIFGTDEKFDLSSDVMKKKVSGFTGQTAGKVTVNKKSIQTPGKTVESLWAKNGP
                     KKSAFKFGVGGTDENYTDYWNTASAYARANPETKESDDKDD"
     gene            35603..36505
                     /locus_tag="ACG8KR_000119"
     CDS             35603..36505
                     /locus_tag="ACG8KR_000119"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HFQ0199528.1"
                     /translation="MLLGSHVSMSGKKMLLGAAEEAASYGSSTFMIYTGAPQNTRRKA
                     IEEMNIDAGKAFMAENGLTNIVVHAPYIINLGNTIKPEMFPFAVQFLREEIVRAEALG
                     ATQITLHPGAHVGAGAEAGIARIIEGLNEVLTKEQTAQIALETMAGKGTEIGRTFEEL
                     AQIIDGVKLNEKLSVTFDTCHTNDAGYNIKEDFSGVMEEFDKVIGLDRLKVLHINDSK
                     NPQGSHKDRHANIGFGTIGFDTLNKIVHDPMFDNISKILETPYVGKDKKKAYAPYGKE
                     ISMFKAQAFDPEVFPELLAEEVTL"
     gene            36716..38491
                     /locus_tag="ACG8KR_000120"
     CDS             36716..38491
                     /locus_tag="ACG8KR_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HFQ0199529.1"
                     /translation="MLNILNPLIDQLKKKHTYFILGIAVLFTIIRLYLYSKAIYGTDM
                     SATYDDQLLIHYAQNILDGKWLGEYTTTTLSKGISYSLFLVLAHKLHLSYAVLFGMFS
                     ILASILVALAFKPIVKNKYLLLSIYFYFLFSPISFTHEYSTRTYRNALVIPTVEIIIA
                     CLLAIYYRKYNSSKKLLPWFLGLAVSFPFFWFIREDSIWLLPFTVVALGISFVQILLG
                     QDFSFKEAKKLKERPFLLSKQEWLKLSLFLLPLVGFSIVYQWIEYKNETTYGIAAIND
                     RSSGEFGRLMKQLIRIDDETNLNKENSEIWVSHAALNKALDASPTFHTVSTRIDELYT
                     THAWTQGGKVKELPGDIIFWALRDVVSESGYYQNNAKETNDFWKKVNQELEEAYQKGT
                     IKKKKEFYLMGSGDGKTMNDLPIVFDFLKSGYAYAWRYKDFHQGGNYSFGTQEQVEQA
                     QELLNLPLMDNWIDSSDPRTPDFQLTKTAKVANVMIKIYQVLAIPVLIASIAGILLMI
                     LGTVFAKADRAYYSSSLIVVIGLILTQLVFLFGVSWFCSYAPEKKDYFLSVYTGAGVP
                     LTQFIAVTSMLVFSKLNLKKALSSK"
     gene            complement(38800..40386)
                     /locus_tag="ACG8KR_000121"
     CDS             complement(38800..40386)
                     /locus_tag="ACG8KR_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HFQ0199530.1"
                     /translation="MNNQSNKQFHKHRLIEHTNGLSLAEVNGTVDVPKGKSFFKTLLA
                     YSGPGALVAVGYMDPGNWSTSITGGQNFQYLLMSVILMSSLIAMLLQYMAAKLGIVTQ
                     MDLAQAIRARTSKTLGVILWILTELAIMATDIAEVIGAAIALYLLFDIPLVIAVFITV
                     LDVFVLLLLTKVGFRKIEALVVCLIFVILFVFVYQVGLSNPDWHSLFAGFIPNSHTFS
                     DSPVIGGETPLTGALGIIGATVMPHNLYLHSAVSQTRKIDHQDEEDVAQAVRFSTWDS
                     NIQLSMAFLVNALLLIMGVAVFKTGSVQDPSFFGLYEALSDPSAMSNGILIAVAKSGI
                     LSTLFAVALLASGQNSTITGTLTGQVIMEGFIHMRIPIWLRRLVTRLLSVIPVLICVL
                     FTSTKGTIEEHTALNNLMNESQVFLAFALPFSMIPLLMMTNSQAEMGSRFKNSFVVKL
                     LGWFSVISLTYLNLRGLPGQIESFFGEQATSAQLALADQIAYVIIVLVLLLLAWTIGD
                     LYKGNKKYEQRLQETIEEIE"
     gene            40637..40861
                     /locus_tag="ACG8KR_000122"
     CDS             40637..40861
                     /locus_tag="ACG8KR_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HFQ0199531.1"
                     /translation="MVSTGIVVLIAIIALLIGAVGGFFLARKYMKDYLEKNPPVNEDM
                     LRSMMMSMGQKPSEKKIRQMMQQMKNQGKK"
     gene            41003..42754
                     /locus_tag="ACG8KR_000123"
     CDS             41003..42754
                     /locus_tag="ACG8KR_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HFQ0199532.1"
                     /translation="MSIFRKLGWFFRQEKKHYLIGIFSLFAVALVQLVPPKVIGIIID
                     EIADKNLSIKIISWWILLLILAAFLQYLFRYIWRTNIWGSAARLEKELRQQLFKHFTE
                     MDNLFYQKYRTGDLMAHATNDLNAIQNVAGAGILTFADSVITGGTTIIAMILFIDWRL
                     TLIALIPLPLLAVTSRVLGSKLHDAFRDSQEAFSSINDKTQESITGIKVIKTFGQEKE
                     DLADFEEKIEDAIIKNKRVNFLDSLFDPFITLIIGLSYVATIILGGKYVLEGTISIGQ
                     MVSFISYIGMLVWPMFAIGRLFNVLERGNASYDRVNELLHEKTHIVEQLGAVQTPAKG
                     ELNVAISSFNYPKEESPALEQIKFAIQEGETLGIVGKTGAGKTTIIKLLMREYDDYQG
                     KIQFGGRSIKEYSLNALLGSIGYVPQDHFLFSMTIRDNIRFSNPKLSEEEVEHAASLA
                     FINNEIKELPKGYDTLVGERGVSLSGGQKQRISIARALIKNPELLILDDALSAVDAKT
                     EEAILHNLKEIRKEKTTIIAAHRLSSVMHAKEIIVVDQGKIIERGTHHELLALGGWYE
                     RMWEKQQLEAKIEGGEA"
     gene            42754..44538
                     /locus_tag="ACG8KR_000124"
     CDS             42754..44538
                     /locus_tag="ACG8KR_000124"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0199533.1"
                     /translation="MDENYQSEWTKSIPLKEQKKIIKRLMKFAKPFRRTFIVAILFAF
                     ALSVINVLLPRIIQTFMDDHLAKQSATTQVILFFAGVYLFGVIVKSIIWFFQWYLYSM
                     ASLKTYQYVRVRLFEKLHTLGMRYFDQTPAGSTVSRVTNDTETLFEFWYVFLMVLTGI
                     FAVVSSFVAMFQINGEIALWTLVFLPILGIVIWYYQKFSSRIYRNMREKLSQLNTKLN
                     ESISGMRIIQQFRQEARLAKEFEQVNNEYLDTRYAMIKTNSILLSPIINFLYALAIAL
                     ALTLFGIDALHSPVEVGLIYAFTTYVQGFFNPMSQMMDFLSTFTDGMVAGSRILKIMD
                     TEEYAPQQNPGADQAITSGKIEFRNVSFSYDGEHNVLNNISFVANPGETVALVGHTGS
                     GKSSIINVLMRFYEFYEGQILIDDHDIREYPMEELRKKMGLVLQDAFLFYGDIAGNIR
                     MMNPAITDEQIRHAAEFVQANKFIDELPKGYHSKVIEHGASYSSGQRQLITFARTIVT
                     DPKILVLDEATANIDTETEGLIQEGLAKMRQGRTTIAIAHRLSTIRDAELILVLDKGH
                     IVERGNHETLLAQNGLYADMYKLQSSGV"
     gene            44873..45724
                     /gene="licT"
                     /locus_tag="ACG8KR_000125"
     CDS             44873..45724
                     /gene="licT"
                     /locus_tag="ACG8KR_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator LicT"
                     /protein_id="HFQ0199534.1"
                     /translation="MLIEKVLNNNVVITRDERNQEMIVMGKGLAFKKKVGDPIFDSCI
                     DKKFQLTNDGLSQQFQELAQAIPLEYLEISCEIIEYTKEALQVQLNDSVYIALTDHLY
                     TAIERHKMGIFVPNVLLYDIKQLFPKEYAIGKKTVKKISDKYHIKLSEDEAGFIALHL
                     VNAQGIQSQQNNTYLFTITIQDILNIVRYYYKITFDEDSVHFFRFVTHLRFFLTRVLD
                     GEKSEVGEVSEELFQVIQKKYKDTFGCVEKIADFLAETFNYQMSTDEKMYLTIHTARL
                     VHENRCK"
     gene            45821..47707
                     /locus_tag="ACG8KR_000126"
     CDS             45821..47707
                     /locus_tag="ACG8KR_000126"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFQ0199535.1"
                     /translation="MGKYQELARKIVENVGGKENINGLTHCITRLRFKLKNEEKANDE
                     ILKNMDGIVTVMRAGGQYQVVIGNHVPVVFEEVIKAGNLTFDEAVSTEKMRPFDMLID
                     GISGCFQPFLAILAAGGMIRGLTAFLVFLGAFDRGSGTFVMFDNIGDSVFQFMPVIIG
                     LTAARKFKVNEFVGMLLGAALMNPSLSLEALSGAAEAPLTTIFSGTIFEAPIYQTVFG
                     IPWIARNYASSVIPIIFIVLLASQIQKPIKKLVPEMIANFFVPFFTVLITMPLGFLLV
                     GPVFTFATDILMAGFETLLALSPVIYGAIVGFFWQILVMFGLHWAIVPMGLMQFSVNG
                     WQNIMTPVAVVSFGQTAALTALYFKLRNPKDKAIAIPAIVSGIVGITEPAIYGFTLPR
                     KKIFIFTCVGGALGGAYSGLMNLTSWNQGGLGIFTIPNYIRPDGDLTDVINVLIGIAI
                     AMVVSFALTFFFWKEEAGETDDRQKRSGKEIVKTPIQGQIAPLKAAKDAAFAQGTLGR
                     GILIYPEKGEVRAPFDGTVMTLFPTKHAIGMVSETGLELLIHVGLDTVQLEGKYFESL
                     VQQGERVKQGDLLLRFDKKKIEKEGYSLETPVIVTNYMDYLDILENQGETVGPDDALI
                     TALT"
     gene            complement(47748..48650)
                     /locus_tag="ACG8KR_000127"
     CDS             complement(47748..48650)
                     /locus_tag="ACG8KR_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFQ0199536.1"
                     /translation="MLLREKIKEISFSPSENEVIQFLLSDSPALGEMTIQEIAERCYV
                     HPSTLIRVAKKMGFDGWVSFRNAFLAETAYLEGNFQDVDANLPFSTTDGIMTIANKMA
                     SLEQMTIQDTLSLIHHDQLQKAKQLMLQAKKIVIFASNSNTLISQDFMLKMKRIKMDV
                     TIVTTFGESFYEAYNCTSDMCGILISYTGENNVILRTAEILKQNHVPILALTSIGDST
                     LSSMSTAALRLTTRERLYSKIGNFTINTSICYLLDVLYSCVFAENYQQNLVHLISVGQ
                     KFDKRPISSAIMKEPFNFENKEVD"
     gene            48880..50283
                     /locus_tag="ACG8KR_000128"
     CDS             48880..50283
                     /locus_tag="ACG8KR_000128"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008706 - 6-phospho-beta-glucosidase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFQ0199537.1"
                     /translation="MAKGIKIATIGGGSSYTPELMEGFIKRYEELPIREIWLVDVEEG
                     KEKLEIVSQMSQRMWDASPYEVKIYATLDREEALKDADFVTTQFRVGQLDARIKDERI
                     PSYYGMIGQETNGAGGIFKAFRTVPVVLAIVEDMKRLCPNAWLINFANPSGMVTEAVI
                     RYGKWDKVIGLCNVPVMATMTEPAMLGKKKEELIYKFAGLNHFHWHKVADNKGNDRTA
                     DLIDRLYDEDNGLPKNIFDIPFFKEQLKQMNMIPCGYHRYYYREEEMLEHALEEYQTI
                     GTRAQQVKETEAELFELYKDPELDHKPEQLQKRGGAYYSDAACETIASIYGNKETQIV
                     VSTKNDGAVPDLPADCVVEVTAYVGAQGARNVAFGSLPAAERGWLQVMKNMELLTIEA
                     AVTGDYGTALQAFTINPLIRSGKTAKRIMDELFIAHQQYLPQFKETISRLKAEGIEVQ
                     DELARELSSNTHVMEEV"
     gene            50283..51716
                     /locus_tag="ACG8KR_000129"
     CDS             50283..51716
                     /locus_tag="ACG8KR_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFQ0199538.1"
                     /translation="MGFRKDFLWGGATAANQCEGGYDEGGRGLANVDVVPIGEDRFPV
                     ITGKKKMFEFDKDHFYPAKIAIDMYHHYKEDIALFAEMGFKTYRMSIAWSRIFPMGDE
                     NEPNESGLKFYEDVFKECQKYGIEPLVTITHFDCPMHLIKEYGAWRSRELVGFYENLC
                     RVIFNRYKGLVKYWLTFNEINMILHAPFMGAGLYFEEGENEEQIKYQAAHHELVASAI
                     ATKIAHEVDPENKVGCMLAAGAYYPYTCNPEDVWASRKSDRENYFFIDVQSRGEYPAY
                     ALKEMERNGIQIKMEPGDAALLKEHTVDFISFSYYSSRVSTTDNELLEQTAGNIFASV
                     KNPYLEASEWGWQIDPLGLRITMNDIYDRYQKPLFIVENGLGAVDTPDENGYVEDDYR
                     IDYLAAHIQAMKDTVELDGVDLLGYTTWGCIDLVSAGTGEMKKRYGFIYVDRDNEGNG
                     TLARSKKKSFNWYKKVIDSNGENLSND"
     gene            51861..52787
                     /gene="trxB"
                     /locus_tag="ACG8KR_000130"
     CDS             51861..52787
                     /gene="trxB"
                     /locus_tag="ACG8KR_000130"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HFQ0199539.1"
                     /translation="MYDVIVIGAGPAGMTAALYASRSNLSVLMIEQGAPGGQMNNTAE
                     IENYPGFDSIMGPDLAYKMYEGVSRFGTENAYGIVQDIKDHGSHKEVICEDKTYEGKT
                     VIIATGCIHRKLGVPGEEEFAGRGVSYCAVCDGAFFRNKRLVVIGGGDSAVEEAIYLT
                     QFASEVVIVHRRDELRAQKIIQDRAFANEKISFMWDTVVDEIVGNEMVVTGVKARNVK
                     TGNEFDLAADGVFIYVGLDPLTAPFREAGLTNEAGWIPTDNEMKTNIPGVFAIGDVRE
                     KDLRQITTAVGDGGIAGQQVYKYLESLEETTA"
     gene            53001..54728
                     /locus_tag="ACG8KR_000131"
     CDS             53001..54728
                     /locus_tag="ACG8KR_000131"
                     /EC_number="5.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HFQ0199540.1"
                     /translation="MSWEQVYEQWVHEENLEENLKKQLIDLGEDPEKLEDAFYAPLEF
                     GTAGMRGILGPGINRMNIYTVRQATEGLARFMDTQGEETKRRGVAIAYDSRHQSPEFA
                     MEAAKTLAHHNIPSYVFESLRPTPELSFAVRHLKTFAGIMVTASHNPAAYNGYKVYGE
                     DGGQMPPADADALTKYVREVSNPLKVEVLSDDEAEHSGLITIIGEEVDAAYLEEIKAV
                     TIDHELVETMGKDLKLVYTPLHGTGKMLGERALKQAGFEQFVLVPEQAVADPDFTTVK
                     SPNPEEHSAFEYAMRLGEKEGADLLIATDPDADRLGAAVRLPNGSYEVLTGNQIGALF
                     AQYILEAHQSRGTLPENAAVLKSIVSSELPTAIAEHYGATMFNVLTGFKFIAEKIQQF
                     EEDHSHTFMFGFEESYGYLVKPFVRDKDAIQALLLLAEVAAYYKKQGKTLYDGLQTIF
                     ETYGYYAEKTISVTMSGQEGSAKISALMEKFREEAPTEFAGAKVIQTEDFKELTRTDR
                     EGNVEKLTTPPSDVLKYTLEENGWIAIRPSGTEPKIKFYIGVKGNSSEDAAEKVAAYE
                     AIIKKMTEE"
     gene            54744..55094
                     /locus_tag="ACG8KR_000132"
     CDS             54744..55094
                     /locus_tag="ACG8KR_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HFQ0199541.1"
                     /translation="MEKVVNFRSQSFFKLEVNGLPDTQKTKVEKVSQLFKMLSDPTRL
                     KILLYLKDGEQNVTAITQAVEMEQSAVSHQLRLLRENHVVKSHREGKAILYSLDDSHV
                     LDILNQTLKHVEHQ"
     gene            complement(55188..56048)
                     /locus_tag="ACG8KR_000133"
     CDS             complement(55188..56048)
                     /locus_tag="ACG8KR_000133"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, water dikinase regulatory protein"
                     /protein_id="HFQ0199542.1"
                     /translation="MSSDSTEIVTFFVISDSAGETATKLAQATMAQYPTVEFNLFRRT
                     FVTDEKTLMQALQDALKEKALVLHTLINQELIEMTRSFCEEHQLFSFDVMTPPISEIE
                     RLTGIKPIRQPGALHLLNENYFKRIKAMEFAVKYDDGKDPRGFLEADVVLLGVSRTSK
                     TPLSLFLANKNLKVANLPLIPQAHIPKQLWEIDPKKIVGLTNNPDILNNIRKERMRSY
                     GLNPDTAYSDIEKIRAELDFANDLYEKLGCVVINVASLSIEETASLILNALDLEDHSY
                     YGTETTEETD"
     gene            56140..56568
                     /locus_tag="ACG8KR_000134"
     CDS             56140..56568
                     /locus_tag="ACG8KR_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HFQ0199543.1"
                     /translation="MKKQTLIEQSLETLKNNGFKYTKKREALLTYLVKRNRYVSAKEV
                     FDYMNQEFKGVSYDTIYRNLHDFAEIDLLEETDLNGEMKFRFHCCQGEIGHHHHFICT
                     VCGTTKEIHMCPMNYFEEQLGGCTIEGHRFEIFGRCENCQ"
     gene            56730..56906
                     /gene="rpsU"
                     /locus_tag="ACG8KR_000135"
     CDS             56730..56906
                     /gene="rpsU"
                     /locus_tag="ACG8KR_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002884573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HFQ0199544.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVSKAGTLQESRKREFYEKPSVR
                     RKKKSEAARKRKKF"
     gene            56933..57379
                     /locus_tag="ACG8KR_000136"
     CDS             56933..57379
                     /locus_tag="ACG8KR_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HFQ0199545.1"
                     /translation="MSLLSRLNDDMKTAMKAKDKESLQVIRMIKSSIQNEQIKEGHDL
                     TEEEELTVLSREMKQRRDSLHEFEEAGRDDLAEKVKSEIVIVEKYMPEQLSDEEIRQL
                     VQEAITQTGASSMKEFGKVMGAIMPKVKGKADGNQVNAIVKELLQS"
     gene            complement(57535..58914)
                     /gene="brnQ"
                     /locus_tag="ACG8KR_000137"
     CDS             complement(57535..58914)
                     /gene="brnQ"
                     /locus_tag="ACG8KR_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HFQ0199546.1"
                     /translation="MEETKTYSWKQLLMVSSLIFGMFFGSGNLIFPVHLGQMAGANWV
                     SAGVGFAISGALFPLLAILAVVVTKSDGLYDLAKPVGKWYAALFLILVHLTIGPFFGT
                     PRTAATAFEMAAKPFLPEKYNSLGMFLFSALFFLLAYFLTVHPTRLVKYVGKYLNSLF
                     LALLAIIFVVSLFHPMGNLNQSAADTYQTNALTNGVLEGYNTVDAVALLALSVTFVHA
                     VRDLGFKDKKVTELTAKAGSLSVALEVMIYFGLVLLGAMSLNKMSLSENGGTALSQIT
                     NFYLGNFGASFLGVMVTLGVFTTAMGLVVSFAQDFHKLFPKVSYMTWLRLTTFVSFVV
                     ANAGLDNIIQWSLPVLMLLYPLSLALILLSLTAKFFQKTPFVYQVTMLFAAVPAVLDM
                     LANSPALVSQQRVVASMLEFYHHHVPFAALGLGWMVPTLLGYAGSLLFYYAYRLSGYK
                     QEANELPEE"
     gene            59620..60591
                     /locus_tag="ACG8KR_000138"
     CDS             59620..60591
                     /locus_tag="ACG8KR_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HFQ0199547.1"
                     /translation="MTEESSSLEIRLTAADDVSMLLGAHDKHIKLIEETTETTIHTRG
                     EIIQIIGEQSQISLAASVIRTLQELIKRGIHVSTPDVVTALKMGQKGTLDYFVEMYEE
                     EIVKDRNGKPIRVKNSGQKKYVESVAKHDIVFGVGPAGTGKTFLAVVLAIAALKKGEV
                     QKIILTRPAVEAGENLGFLPGDLKEKVDPYLRPVYDALYQIFGLDHTNRLMERGVIEI
                     APLAYMRGRTLDDAFVILDEAQNTTVAQMKMFLTRLGYQSKMIVNGDTSQIDLPRGTT
                     SGLVHAERTLKQIKKIDFVNFEASDVVRHPVVAEIIKAYEKADLHQE"
     gene            60615..62807
                     /locus_tag="ACG8KR_000139"
     CDS             60615..62807
                     /locus_tag="ACG8KR_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing metalloprotein"
                     /protein_id="HFQ0199548.1"
                     /translation="MLNKTLQKLQKKLGKAALPFLLFLFFIIVCGVTFSSVKQKANDF
                     REGQVAEESIRANKTIENTEETEQRRKLAAEAVTPEYTYQKDLADDQNNRIKQLFELI
                     DKTNDTINKSYNDKVDKAKEDENIPKPTTEERIATLKKNFENVNAENVAFYQKLPNNF
                     YSTIFEMTETEIHTVRDESLKLIDEQMSKQVRESELEAFKQEAEDQIQYLNVTTEQQQ
                     MIRYLVDQGIVVNDVLNEKKTEELKQSAREAVQPVMIFQGEIIVREGNQIDASAMKKL
                     ELLGLTNQTTSIFPLIAMVLAVLLQIGVLIYNSLQFQNAGERTKYVLFYVTAMSVSVV
                     LMKFFQLFQTEQAAYIPLFYPAAFAPLVLSFFLNRRAGIMAGIFQAVSALFIFYDSIG
                     TNFLTVILMSYVFSGLMATIVRRKRISEQGLSAAMWVIIFPVMMDVILIIYQGMSFGD
                     ATTWLTLVCGFTGALFSFLLTIGLHPYIELMVNDDSVIVLNELSNPNHPLLKQLLEEA
                     PGTYHHSMMVANLSANAVAEIGGRSLLTRVACYYHDIGKIKHASFFVENLPSGAENPH
                     NFLLPEDSKHIIFGHVTDGAKILEDYNMPQMVIDICRQHHGTTLMRYFYVKAKERNPE
                     VTEEQFRYPGPKPQSREAGIVNIADSCEAAVRAMDHPTSEKIESFVHNLIEERISDGQ
                     LDDSGLTLKEIRKVEKSLISGLSSTFHSRIKYPKMKSEVEKIKEEQEE"
     gene            62824..63300
                     /gene="ybeY"
                     /locus_tag="ACG8KR_000140"
     CDS             62824..63300
                     /gene="ybeY"
                     /locus_tag="ACG8KR_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HFQ0199549.1"
                     /translation="MDITFMDETNQVSEEKMKEIDDLLQFAASYLELPEETEMSVTFM
                     DNAAIQVINRDYRGKDAPTDVISFALEEEGEDEIPVIFEDGENPLPRVLGDIMISTER
                     AAEQAEAYGHSYDRELGFLAVHGFLHINGYDHMTPEDEKEMFGLQKEILDAYGLKR"
     gene            63284..63679
                     /locus_tag="ACG8KR_000141"
     CDS             63284..63679
                     /locus_tag="ACG8KR_000141"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFQ0199550.1"
                     /translation="MDLKDKNVEKNKHFLMSVEFAVQGVKTVFEEERNMRKHVAFGIL
                     ALFMGVIFQLDRIEWLWLLLAVFLVWIVEIINTVFENVVDMFTDFHFHPIGKKIKDMA
                     AGAVLVTACFSVIVGLILFVPKIYHLLFN"
     gene            63694..64593
                     /gene="era"
                     /locus_tag="ACG8KR_000142"
     CDS             63694..64593
                     /gene="era"
                     /locus_tag="ACG8KR_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HFQ0199551.1"
                     /translation="MPEHKSGFVAIVGRPNVGKSTLLNRIVGQKIAIMSDKAQTTRNK
                     IQGVYTTPEAQLIFIDTPGIHKPKHRLGDFMVEAAYSALKEVDAVLFMISADQKRGRG
                     DDFIIERLKNVHSPVYLIINKIDKIHPDELLGIIEDYSTQMPFAQVIPISATEGNNVE
                     RLMDVLVSEMPEGPQYFPDDQVTDHPEYFIVSELIREKVLFLTRDEVPHSVAVVVDSM
                     KRNENDKIHIQATIIVERDSQKGIIIGKGGKMLKQVGTKARKDIENLLGDKVFLELWV
                     KVQKDWRDKKVYLQDFGYRKDEY"
     gene            64736..65548
                     /gene="recO"
                     /locus_tag="ACG8KR_000143"
     CDS             64736..65548
                     /gene="recO"
                     /locus_tag="ACG8KR_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HFQ0199552.1"
                     /translation="MSQLAESKGLILSSRNYKEKDKLVKIFTESSGKMMFYIKGIHRQ
                     NQPLAPALLPFTEAVYIGNFRSEGLSFLSSVKDVHAFRHIQEDIFLSGYGTYLLNLVD
                     AAIEDHQYDPHLFQFTQQALQLMDQGDDAEIITNIFEVQILQRFGIAPIWTHCVVCGE
                     TKGKFDYSSKYGGVICERHWSMDDHRYHADPRAVYFVRMFSAISYDKISGIHLKEETK
                     QAIRELIDQLYDEYVGIHLKSKKFIDQMKSWAHVLKTSEKDMSEKDLPKKEE"
     gene            65934..66851
                     /gene="glyQ"
                     /locus_tag="ACG8KR_000144"
     CDS             65934..66851
                     /gene="glyQ"
                     /locus_tag="ACG8KR_000144"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HFQ0199553.1"
                     /translation="MSKKLTVQDMILTLQKFWSSNGCMLMQAYDTEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPENIQELYLESLRL
                     LGIDPLEHDIRFVEDNWENPSMGCAGVGWEVWLDGMEITQFTYFQQVGGLPCKPVTSE
                     ITYGLERLASYIQEVESVYDLEWADGVKYGEIFKQPEYEHSKYSFEVSDQDLLLSNFD
                     RFEKEAKRCIDENLVHPAYDYILKCSHTFNLLDARGAVSVTERAGYLSRIRNMARAVA
                     KIFVAEREKLGFPLLNKNTANPENVKEAE"
     gene            66853..68928
                     /gene="glyS"
                     /locus_tag="ACG8KR_000145"
     CDS             66853..68928
                     /gene="glyS"
                     /locus_tag="ACG8KR_000145"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HFQ0199554.1"
                     /translation="MAKDLLLEIGLEEMPAHVVTPSMKQLEEKTAKFLDENQLTYDTI
                     ETFSTPRRLAIKVTNIPERQADSEEEVKGPAKKIALDADGNWTKAAEGFVRGQGLTTD
                     AITFRELKGVEYVYVTKYTHGKESKEVLTGLADVIKSLTFPVTMHWAEYDFEYIRPIH
                     WIVALLDDEVIPFEILDVKTGRASRGHRFLGEDVTFAHAKEYEEKLNEQFVIASPQKR
                     QEMIVTQANELAEKNHWVIDLDEDLLEEVNNLVEYPTVFSGDFEEKYLSVPEEVLVTS
                     MKEHQRYFEVRNDQGMLLPHFISARNGDRVKLENVVRGNQKVLTARLEDAEFFYNEDK
                     KASIDQYVEKLKTVTFHEKIGSIYEKMQRVQVIARIIGEQVGLSEEELMDLQRAAEIY
                     KFDLVTNMVGEFPELQGIMGEKYALLKGEKPAVATAIREHYMPISSEGELPQSTVGSV
                     LAIADKLDSIFSFFAVGMIPSGSNDPYALRRQAYGVVRIIESKKWAFPLSVLQETISD
                     VISKDTDRFGIGLSEGQQQVIDFIKGRLRQLLTTKNIRHDVIDAVLNAEQKDLTKVFA
                     AAQLFKQHLADEDFKPSMEALTRVVNLAKKAQLEKQSEVDPELFENDAEKELHKAVEA
                     MKASFADQTIHENYDQLVALRPLIENYFDQTMVMADEPAVRKNRLTQLSQIAAMALSL
                     ASLDQIITK"
BASE COUNT        22639 a        12281 c        14834 g        19394 t
ORIGIN      
        1 ttttttttgc tttgctacct tgttccttga agattctgct gatttaagat agagttaagt
       61 taactacaag aattggaggg atccacatga aatggaaaga aaaagtagac gaagcagctg
      121 aaaagttata tgatttggtc aaatcagaaa aatacaatat tgaagtgaat attccgaaaa
      181 aaggcggaaa agcggtccag gtaaaatcaa aacgcccaac gaatcataca aaaaaatgga
      241 tggctaaaaa ccgctaataa taacaaaaga tccataagtc ggcattttca tcgattttag
      301 ggtcttttgt tattttctag gttgaacata tctaaaaaat tgtttctgac aataggagta
      361 ggaagaacga tgtgtgtcta aatcctctgt tgatggatag aaacctttat ctttttcagt
      421 tgtgaatcag gtatatacca gttataatag aagaaacgaa aggacgggtg tacgttatgt
      481 cgaatcaatt acctgtatat atacagattc atgaccaatt aaaaaatgaa atcgaacaag
      541 gtatatggaa agtcggagac cgtcttcctt cagaaagaga attagcagtg aaattcaatg
      601 tcagccgtat gacgttgaga caagcaattc aaaacttggc agatgaagga atcttagaaa
      661 gaaagatcgg ttcgggtaca tatgttgcgc gagaaaaagt acaagaaaaa atgtctggtg
      721 caacaagttt tacagaaatt atggaatcgc aaaatcgagt accgtctagt cggacaatct
      781 cttatttctt gacttcgcca agctcaagtg aaatggaaaa actaaacttg agtaaagatg
      841 acacgatctt gcgaatggaa cgtattcgtt tcgcagatga tgtccctatt tgttttgaag
      901 ttgccagtgt gccacaaaag ctgatcgatg gctacagcaa agcagagatt actgaatcgt
      961 tgtatcgcac gctagaagaa aaaggcggga aaaagatcgg tccagcgaat caaacgatta
     1021 ctgctgtgtt ggcttctgag aaaattgctg actatttaga ggtaaaacga ggagatgcag
     1081 ttcttcgttt gcgccagatt tcttattttg aagacggtac gccgtttgag tatgtgcgta
     1141 cgcaatacgt aggaaatcgt tttgagtttt atttggaaaa ataaaaaaca ggtgatccct
     1201 gattccttac ttagaaataa aagagaatca gaatcacctg tttttatata tgatcagtta
     1261 ccagttgctc gtgataagaa acaggaccct cttcaaattg gacttgtccg ttcagcagct
     1321 caatgatgga ttctttaaag tggtcagcac ttttttcatc tacagcacag atgacgctaa
     1381 cttgatcagt atattggatt tcttttataa aaaaatgttg gagttcaaga taattctgta
     1441 attttcctag ttgcggatac gtgatcatca cagagatctc ctgttgcaag gtaccttcta
     1501 cgattccaat gtcagacaat gcttgagaga cagcatgagt gtatgctcgt attaatccgc
     1561 ctgtgccgag tttcgttcct ccaaaatatc gagtgactac tgcagctaca ttgatcagtt
     1621 gatttttttt gaggacttcc aacatcggta cgccagccgt tccacttggt tctccatcat
     1681 cgctgcttcg ctggatttca tttttttctc caatgatgaa ggcgctgcag ttatggttcg
     1741 ctttccaatg ttcttttttt atttcttgaa tgaaactttt tgcttggtcc tctgattcta
     1801 ttcgtctaag agaacagata aagcgtgatt ttttgatctc aatctcactt tgtccatttt
     1861 cttttattgt acgatagcgc tcgatcatct tgtcacctct ttttttcatt ttctttattc
     1921 agtataaata aagttctagg ttgtttcaac actgtaacgg ttgcgcaatc gctgtagtat
     1981 gcgataataa agacaaaggt aggtggatct ggggatgcag gaacgaatga aaaagtacga
     2041 tgcaattacg cattatttga agaacaatgg cggctctcaa gtaacgttga cctttaccca
     2101 atttgacgaa ctgctgtttc ccagtaacgg acttcccaaa acagcaaggg aaagtacaga
     2161 ttggtgggca aatgattata aacaccctga aaaaggtgcc tatggctgga taaatgcagg
     2221 ctatgaagta gttgtcatca atttggacaa agaatatgtc gtgttcaata aattagtgaa
     2281 atctagttgg ctatttgatt aaataaaccg cgtttagaaa acgttgacaa atttctttct
     2341 ttttatataa tagagtaaca gacgcgaaga aaacgtttgc gtagtttgtt ttaaaaagga
     2401 gagaaaagta tggctaaaaa aacaatcatg cttgtatgtt cggctggtat gagcacaagt
     2461 ttactagtaa caaaaatgca aaaagctgct gaagcaaaag gaatcgactc ggatattttc
     2521 gctgtgtccg cttctgacgc agataacaat cttgcaaata aagatgtaga tgtactgtta
     2581 ttaggacctc aagttcgctt tatgaaagct gattttgaaa aacgtctgga accaaaaggc
     2641 attcctttag acgtcatcaa tatggctgat tatggtatga tgaatggcga aaaagtgtta
     2701 gatcaagcaa tcacgctcat aggtgaataa atagaacagt ataactatcg aacaagcgat
     2761 ccaaaaaata gatcgcttgt tttttcgtat atttttttag ggcgaataat atgaatcatt
     2821 tttggggtag aaggcttctg acgagagata ttaaagcaat agactgtgag gaggtaaatt
     2881 taccaaatta tgagaagatc gcagcagtgg aacgtttggg caactggatt ctttgtcatc
     2941 gctgtggaat gaggacgtcc gtcattgatg gacagcttgc agattacggt tatttttgta
     3001 cacattgtct atatttaggg cgatgcgaca ctcaacaaga tttatatttg tttgatcagc
     3061 ctgaagcaaa acctcgagaa gtcgtctttt tttggacagg acagcttact gaaaaacaaa
     3121 cggaaatcgc aaagagaata ctttgccatt cagaaaaaag acatcatctt atttgggctg
     3181 taacaggtgc agggaaaaca gagatgcttt atcctatttt agtggaaact ttaaaagctg
     3241 gaggaagagt agcgatttgt acaccgcgag tcgatgtttg taatgaattg tttttacgct
     3301 atcgttatgt ttttccagaa gaaaaaatca cgttgctcca tgggaataca gcaacggctt
     3361 atacctttac ttcttttgtt atctgtacga ttcatcagct ataccgtttt tatcggtcct
     3421 ttgatttaat agtgatcgat gagatcgatg cgtttcctta taacggggat gcaggattag
     3481 ctcatgctgt gcaaacagcg attaaaccag acggacgatt catctactta agtgccacac
     3541 ctgataaaaa gttactaaag gaaatcaagc agacgtttga catccacaaa cttgctttgc
     3601 gctttcatcg gcgtttactt cctgaacccg tcttggtttt catgaataat tggcgacaaa
     3661 aatgcaagaa caagaagaag ataaaatctt tggtgcggat cattcatcaa ttaattaaag
     3721 aaaaccatat ccttttattt tgtccaagta tcacgatgat gcatcgtttg aaagccacac
     3781 ttcaagaggc tcttccgaga tatgagatca cagatgtttc ttcaagagac aatcagcggg
     3841 cggaaaaagt ccagaaaatg cgagaagcaa aatatgacat actgttaacc acgatgatcc
     3901 ttgaacgtgg agtcacattt gaaagaatat ccgttatcgt tttaggagcg gatcatccgg
     3961 tttttactaa atcttctttg gtacaaatcg caggaagagc cgatcgtaaa gggccttata
     4021 cgaacagtca agtatacttt ttctatgaag aaaaaactgc agctattcga aaagcttgtc
     4081 aggaaatcaa agaaatgaat gaagaaggga agaagatgct gtgaggtgca gttgctgcag
     4141 taaagatatc gtccaaaatt taacattcag cgagattatt ggtgtttcga aatacagatt
     4201 acctcagact tgttcagttt gtcgcgcaca atttcattta ttggaaacaa gaggctgttc
     4261 tggctgtcaa aaaccaatcc atttcaatct agagagtacg aaaaagaaag taaagtctat
     4321 tcccgtttac tgcagtgact gtcaagaatg gaagaagaaa tatccgacct atgattttca
     4381 gcaccgtgcc ttttttgtgt acgatgaaaa tatgaaggcc tggctcaatc aatacaagtt
     4441 tatgggagat atccgactag caggcacctt tgcatataaa tggacgcaga taaaaaaaga
     4501 atacccgaca tacatctatt gtccaatacc tttatctgaa aaaaggcgaa cagaaagagg
     4561 attcaatcaa gtaagcgaga tgttgaaagc agcaagaatt ccttttcaga tgctactcaa
     4621 aagagagaag catctgctgg ctcaagcaaa aaacacgcga aaggaaagat tggagatgcc
     4681 tcaacctttt gaacttgcag tcaccttttc taagatacaa aataaaaaaa tattgattat
     4741 tgatgatgtc tatacaacag gacagactct tttccacgca gcagattgtt tgcaggtcgc
     4801 acagccgaaa atcatccgaa ccatttcttt agcaagatga attagatgtg gaaatttgat
     4861 agcgatttaa tttgcaaata acctagagtt cgatataata gaattaagaa gagggaaaga
     4921 gtggtccttc tccttcttga ttgtggtaga cgaaaggggt aatttttatg tttagatata
     4981 atgtacgcgg cgaaaatatc gaagtaactg aagcgattcg cgactacgtt gagaaaaaag
     5041 taggcaaact agaacgctat ttcagtgacg cacccgatgc aacggcgcat gtgaacttaa
     5101 aagtttatac agaaaaaaca gcaaaagtgg aagtaacgat cccattacct tatcttgtat
     5161 tacgtgccga agaaacttct ccagatttat acgcaagtat cgatttggtt gttgacaaat
     5221 tggaacgcca aatccgcaaa ttcaaaacaa aaattaatcg taaatcccgt gaaacgggca
     5281 tgaatactgc agatgctact gctttattca atgaagagga cgaaaacgaa aatgattctg
     5341 aattagatat cgtacgtacg aaacgccttt cattgaaacc aatggatagt gaagaagcag
     5401 tcttacaaat gaacatgctg ggacataact tctttatttt tgaagatgca gaaacaaatg
     5461 gtacaagtat cgtttaccgc cgtaaagatg gaaaatacgg actgattgaa acagactgat
     5521 cagccttact taaataacaa cgaaaacttg cagcagctac tgacagctgc cgcaagtttt
     5581 tttgagtttc ctcctctttt agatgaaata tgaataaatt atgaagttct tactttaata
     5641 cgcttaagat atttcaatta tctataacta atgataaaat gaaaagtaaa gtctacatag
     5701 tctggataat acacagaagg aaaaggagat tacacaacgc atggccaatt tgattcgttc
     5761 acttatcgaa aacgataaga aagaactaaa acgtttagat agcattgcaa aaaaagttga
     5821 agcatacgct gaccaaatgg cagcattaac tgatgaacaa ttacaggcaa aaactgctga
     5881 attcaagggc agataccaaa aaggagaaac attagatcaa ttacttccgg aagcttttgc
     5941 agtcgtccga gaagcagcaa aacgtgtatt ggggttatat ccataccacg tacaattaat
     6001 gggtgggatc gtgcttcacg atgggaatat ccctgaaatg cgtactggtg aaggtaaaac
     6061 cttgactgca acgatgccgg tgtatctgaa tgcgttgact ggcgaagggg tccacgtagt
     6121 taccgtcaat gaatatttag caacccgtga ctcgacggaa atgggcgagc tttataattt
     6181 cttaggattg acagttgggt tgaatatcaa ctcaaaatct tcggatgaaa aacgcgaggc
     6241 ttataattgc gatatcactt atagcacgaa taatgaatta ggatttgact atttgagaga
     6301 caacatggtc gtttaccgtc atcaaatggt acaacgtccg ctgaattatg ccattgtcga
     6361 tgaagttgac tcgatcttga ttgatgaagc gagaacgccg ctgatcattt cagggcaagc
     6421 agaaaaatca acagctttat atacaagagc agacaacttc gtgaaacgtt tgaaagaaga
     6481 agaagattat aaaatcgata tacaatcaaa aacaatcagt ttaactgaag cagggatcga
     6541 aaaagctgaa gaaacattcg gtttggacaa cttatatgac attgaaaata ctgcattgac
     6601 ccatcacttg gatcaagcat tgagagccaa ctacatcatg cttttagaca tcgattatgt
     6661 tgttcaagat ggaaaagtat tgattgttga ccaatttacg ggtcggatca tggatggacg
     6721 ccgttattca gatggtcttc atcaagcgat cgaagctaaa gaaggcgtag agatcgaaga
     6781 cgaaacgaaa acgatggcga cgatcacttt ccagaactat ttccgtatgt acaagaaact
     6841 tgcggggatg actggtacag ctaagacaga agaagaagag ttcagagaga tttataatat
     6901 tcaagtatac cagattccga caaaccgtcc ggtcatccgt gacgatcgac cagatttgct
     6961 ttacccaaca ttacaaagca agttccatgc tgtcgtacaa gatatcaaag atcgctaccg
     7021 caaaggacag cctgtcttag tcggaactgt tgcagttgaa acatctgagt tattatcaga
     7081 tatgctgaat caagaacgta ttcctcatga agtactgaat gcgaaaaatc actttaaaga
     7141 agcagaaatc atcatgaatg cgggtcaaaa aggtgcagtg acgattgcta ccaacatggc
     7201 tggtcgggga acagatatca agctagggct tggcgtaaga gaacttggtg gcctagcagt
     7261 tatcgggaca gaacgacatg aatctcgtcg tatcgataac cagcttcgag gacgtgcagg
     7321 acgtcaagga gaccctggta tgtcacaatt ctacttgtct ttagaagatg atttgatgaa
     7381 acgattcggt tcagaacgaa tcaaagcttt cttagatcga atgaagatcg gtgacgaaga
     7441 tgctgtgatc caaagcaaga tgctgacgaa acaagtagaa gctgcacaaa aacgcgtaga
     7501 aggaaacaac tacgacacac gtaagaacgt cttgcaatac gatgacgtga tgcgtgagca
     7561 acgtgaagta atctataaac agcgtcaaga agtgatcatg gaagaaaaaa gcttatctaa
     7621 accactgatg aatatggtca aacggacaat cagtcgtgtg gtagatgcgc atacgcaatt
     7681 agaaaaagaa aattggaatt tggaaagtat cgtcgatttt gctggcagca cgcttgtcca
     7741 tgaagacagt atttccctta cagatatcga aggaaaaacc tctcaagaaa tcaaagaata
     7801 tctaaacgaa cgagcacaag aagtattcaa taccaaagca gctcaattga acggaccaga
     7861 gcaactgctt gaatttgaaa aagtcgtgat cttacgtgtc gtggactcta aatggacaga
     7921 tcatattgat gcaatggacc aattacgtca gtcaatcggc ttgcgtgcct atggtcaaaa
     7981 caatccatta gtcgaatacc aaacagaagg ttataagatg ttcgaagata tggtaggtgc
     8041 cattgaatat gaagtcactc gtatcttcat gaaagctgag atccgtcaaa acgtacaacg
     8101 tgagcaagtt tctcaaggaa cagcttctca ctcagaagat ggagatgcca aagaaaacag
     8161 taatactagc gcgaaaaaac aaccaatcca tgtgaacaag gttggtagaa atgatccttg
     8221 tccttgtgga agcgggaaaa aatataagaa ctgtcacgga aaagatgcaa actaatttct
     8281 gacagcagga ttggtgagac aaattctttt ttaaagaatt tgtctcacct tctttctgtt
     8341 atttgacgat tctgttagac tagatataat aaaaaaagta tagtgaaaga aggaaaaaaa
     8401 catggaaata tccgaaattc gaaatctcct gacagaaatg caggaaaagg tcacaagctt
     8461 caggaggtct ctttgactta gaccagctgg aagaagacat tgcggaagca gaagcaaaaa
     8521 tggctgaacc tggtttttgg gatgatagcg aagctgctca aaaagtgatc agtgaaaaca
     8581 atgcaaataa agaaacttat gatcaattcc attctcttgc tgaagaactg gaagaaatcg
     8641 aattgatggt ggagatgttg caggaagaac ctgatgcagt aatgcaagaa gaagcagaag
     8701 cacgtatcaa tgcgctagat gaaaaaatga atacgtatga attatctatg ttgttagatg
     8761 gtccttatga tcgaaacaac gccatcgtcg aattgcatcc aggggctggc ggaacggagt
     8821 cccaagactg gggcagcatg cttttacgca tgtatacacg ctgggcagag cagcatgggt
     8881 atcaagtaga gacactagat tatcaagcag gcgatgaagc aggaatcaag agtgtgaccc
     8941 tactgatcaa aggacacaac gcatttggct atctgaaatc agaaaaaggt gttcatcgcc
     9001 ttgtacgtat ctcgcctttt gactcagcaa aacgtcgtca tacttctttt tgttcggtag
     9061 atgtgatgcc agagcttgat gaaacgatcg atatagaaat caatccagat gatttgaaga
     9121 tcgacacata tcgggcgagt ggtgcaggtg gtcagcacat caataagaca gaatccgctg
     9181 tacgaatcac tcacttgcct acaggaacag tagtagcgag tcaggcacaa cgttcacaat
     9241 tgaaaaaccg ggaacaagcg atggggatgc tgaaagctaa gctttaccaa ttagaagtgg
     9301 aaaagaaaga acaagaagca gctgcactga gaggagaaca aaaagaaatc ggctggggat
     9361 cacagatccg ttcttatgtt ttccatccat attctatggt aaaagaccat cggacaaacg
     9421 tagagacagg aaatgtccaa gctgttatgg acggagatct tgatttattt attgacggct
     9481 atttgaaaat gcatctgcaa taagagccct ttgttaaatt gaaataaaat tgtaagattt
     9541 tgcaagcaag ttgaaacatt gccatgctat aatagctaaa gactgaaaaa gatatggaga
     9601 gaacgccatg attgaaatga aggatgtaat gaagaagtac tcaaacggta ccactgctat
     9661 ccgcaatctc tcgattgtca tcgaacaagg agagtttgtg tatgttgttg gtccgtctgg
     9721 agctggtaaa tctacattca tcaaattgat gtatcgagaa gaaaaagctt caaaaggaac
     9781 tttgaacgtc gcaggtcatg atctaatagc catcaaaaac cgcgaagttc cttatctacg
     9841 cagagaaatc ggcgtggtgt tccaagacta taaattatta ccaagaaaaa cagtatatga
     9901 aaatgttgcg tacgccatgc aagttatcgg cagaaaacct cgggatatca aaaaacgagt
     9961 aatggaagtg ctggatcttg ttggtttgaa acacaaagtc cgagtctttc ctagcgaatt
    10021 atctggtggg gaacaacaac gtgtttctat tgcccgagca atcgtaaata cacctaaagt
    10081 acttatcgcg gatgaaccga cagggaatct agatccggaa aactcatggg aaatcatgaa
    10141 actattagat cggatcaatg cacaaggaac aactgtcgtg atggctacac ataacagtac
    10201 gatcgtaaat acgattcgac accgagtaat tgctatcgaa aacggccgca ttattcgaga
    10261 ccaagcggaa ggggaatatg gatacgatga ttagaacctt tttccgccat ttattagaaa
    10321 gtatcaagag tttgaaacgt aacggctgga tgacagtagc ttcggtcagt gcggtaacga
    10381 tcacgttgac tttagttggt gtgttcatgg cggtcatcat gaacgcaacc aaactagcac
    10441 aagatataga aggaaacgtg gatgtatctg tgttcgtaga cattggtaca aaagacgaag
    10501 agatgaaaaa attagaaaaa gaattaaatg acttagatca tgtcaaaaaa gtcgaatttt
    10561 caagcaaaga acaggaattg aagaaaatcc aaaacgaaat gggcgattct tggaaattgt
    10621 ttgaagggga taataacccg ttatatgatg tatacgtagt aagtgctacc gatccttctt
    10681 atacaaaaca agtagcagaa aaagcggcag atttgaagaa tgtttcccgt tctgattacg
    10741 gtggggcttc ctcagatcgg attttccaaa ttgctggtgc agttcgaaca tggggattag
    10801 ttgcggcggc attgctgctg tttgtagcaa tgtttctcat ttctaacacg attcggatca
    10861 cgattttatc tagacagcgt gaaattcaaa tcatgcgttt agtaggggca aaaaatggat
    10921 atatccgctg gccatttttc cttgaaggtg gttggatcgg cttaatcggt gccattcttc
    10981 cgatactgat cattacattt ggttacgcat ggttttacca attgatcaat ccgtcattat
    11041 tacgatctca ttattcattg atccatccag aaaatatcgt ttggaaaatc aatcttttga
    11101 tggtcggtat tggtgtgatc attggttcac tcggctcgat catctctatg agacgtttct
    11161 tgaaattcta gtgtcacaag ataaatataa tcagaaagtt ggacgatttc gttccagctt
    11221 tcttttttta tcagctaggt ggagtaaatg gggcggcttt tttctcttta ctttaaaaaa
    11281 caacttgggt attcttaagt tggtagaaaa agaggtgaag ccgtgaagat tatcgttgac
    11341 aattttttta cgatcctttt gattatgaat attttgcttt cctttattat cgtttttcga
    11401 gaaagaaagg aaactgcgca aacatgggca tggctgctcg ttttgatgtt cattccagtt
    11461 gtaggattta ttttatatat ttttttagga agagggattt ccaaagagaa aatctttgat
    11521 ttgaagatcc aagggaaaat cggtaagaat ctagaaatcg aagaagataa acaagcattg
    11581 atgcgagggc tttatcctca tccgcctact ggacaagtag atgtgaaaca attgatctat
    11641 atgcttactg tatttgaaag tacgctttat acgactaaaa atgaaatcac tttatttaca
    11701 gatggtcgtg aaaaattcga tgcgctgatc gaggatatca aacaggcaga agaccatatc
    11761 catttccaat attatattta ccgcagtgat acattaggtg aagaagttcg agaggcattg
    11821 acagaggcag caagacgcgg agtaaaagtc agagtccttc ttgatgcatg gggttccaca
    11881 caagtttctt ctagtttttt tcagaatttg aaaaaagcag gtggagaagt cgcatttttc
    11941 tttcctctat tcgttccgta tatcaatcct cgaatcaatt atcgcaatca tcgtaaaatc
    12001 gtagtgattg atgggaaaat cggatataca ggtgggttca atgttggaaa cgaatatcta
    12061 ggattggtaa agaaatttgg ttattggcga gacaatcatc tgcgtatcta tggagaagcg
    12121 gtttacacct tgcaaaaccg ttttttgatg gactggaatt ctcagcatac aaaagaagta
    12181 ggatattcac cggaattttt cccgaccatt cattctacag gtgatatggc tgttcaaatc
    12241 gtgacaagcg gaccagatac cgaacatgag caaatcaaaa tgacatattt gaaaatgatt
    12301 tcgttagcca aacgggagat attgatccaa acgccgtatt acataccgga tggatcgatt
    12361 catgaggcat tgaagttggc tcttctctca ggcgtaaaag tccatatcca aatacctaac
    12421 aaaccagatc atcttctcgt ttactgggca acgtattctt tcgctgcaga gctgatcgag
    12481 tatggggcaa gaatcgaaac atatgaaaat ggatttatcc atgcaaagac gatgattatc
    12541 gatggcggca tcgtttccgt tggttcagct aatatcgacg ttcgctcatt tagattggat
    12601 tttgaagtca atacactgat ctatgatgaa cgaatggctt ctcgcgtaag aaaagcattc
    12661 tttgaagatt caaagatttc gacgcatctc acaaaagaag tgtatgaaaa ccgagggatg
    12721 atgatcaaga tgaaagaagg attagcaaga ctgatttcgc cgctccttta aaatacaaaa
    12781 aggacaggag agccatcaat agatggaaat ctcgcgtcct tttttctcgg ggccatttct
    12841 gagagtacgt cagaaatgga gaaagcgtct cgcatgagcg aaattaactt tggggtatct
    12901 cttgtttttt ggggaaagca agaaataagc taatatatgc tattaaaaga gacacatttc
    12961 aaagccagta tagcatagaa acttttaatg agatagcttt ttttgcaatt atgagaattt
    13021 attttgttat ttaatgattt tgattcgttt tcatcaagta atgaaaatga tagaataaac
    13081 atactttagg attaaaggag atttctaatg aaaaaactaa aattattctt accaataata
    13141 ggattagcta ttcttttatc aggctgttca aagtggattg atcgaggaga atccattact
    13201 gcagttggct cttctgcctt acagccttta gtagaaacgg ttgctgaaac ctaccaggca
    13261 gaaaatcctg gaaaattcat caatgtacaa gggggcggaa gcggaactgg gttgtctcaa
    13321 gtacagtcag gagcagtcga tatcggaaat tctgatttgt tcgcggaaga aaaatcaggg
    13381 attgatgctt cggcattagt cgatcatcga gtagcagttg ttggaatcac tcctattgtg
    13441 aacaaggata ttggcgtaaa agatatcagt atggaaaacc tgaaaaaatt atttacgggc
    13501 gagatcaaaa actggagtga agtcggtggg aaagatcagc cagtagttat attgaaccgt
    13561 gcatcaggaa gcgggacccg tagtacgttt gaaaaatggg tattggacgg agaaacagct
    13621 gttcaggcac aagaacaaga ttcaagcgga atggtgcgcc aaatcgttgc ggacacaccc
    13681 ggagcaatca gctatgttgc tttttcttac gtttccgatg atgtggatac gttgaacgtt
    13741 aacggtgtgg aaccaacaga tgaaaatgtg acaacgaaca aatggacgat ttgggcatat
    13801 gagcatatgt acacaagaga ccatccaaaa aaactgacaa aagaattttt agactatatc
    13861 ttatctgatg aagtacaaga taatattgtc ggagagctag gctatatccc tgtttcacat
    13921 atgaaagttg aacgtgattg gcaaggaaac attgttaaag agtaatcttt acacaaaatt
    13981 tacaaactta gctaaacttt tacatgttat taaaaccaaa ttgatgttca atttgtacac
    14041 tgagtgagta tgttaaacct gattaagggg gagtcatctg tggaagatgt gaaaaaagta
    14101 ttattaacaa aatcaaaacg ttcaaaaatg gaacagcgcg gaaaattcat tagttttttg
    14161 tgtatcgcgc tgatcgtgtt ggttgtatta tctatttttt attttgtcgc tagtaaagga
    14221 ttggccacct tttttactga taagatcaat gtatttgatt ttctgtttgg tacagattgg
    14281 aatccaagcc aaataggagc aaatgggaaa cccatggtag gggcattgcc tatgatcaca
    14341 ggttcattta ttgtgacctt tttatctgct attgttgcca caccatttgc tattggagca
    14401 gcagtgttca tgacggagat ttctcctaaa aacggaacga agatcttgca accggtcatc
    14461 gagctacttg taggtattcc gtctgtcgtt tacggattta tcggtttatc tgttatcgtg
    14521 ccttttgtca gaagtatctt tggcgggaca gggttcggta tcttagctgg tacatttgtg
    14581 ttattcgtta tgatcttacc gactgtcaca acgatgactg tggatgcatt aaaggcagtg
    14641 ccgcgacact atcgggaagc ttcattagcg ctaggtgcca caagatggca aacaatctac
    14701 aaagtggtgc tgcgagcagc agtgcccgga attttaacag cagtcgtttt tggtatggct
    14761 cgtgcgtttg gcgaagcctt agctatccaa atggttattg gtaatgcggc attaatgcca
    14821 acaggactta ctactcctgc ttctacattg acgtccatct tgacaatggg aatcggaaat
    14881 acaatcatgg gaacagttga aaataatgtg ttatggtcat tagcgctgat cttgctattg
    14941 atgtcattat tctttaatat cgtgatccgt atgattggga agaaaggaga gctgaaataa
    15001 tgaacgcaaa acgctcagat aaaattgcta caggtgttct atatgtggtt tcagggatca
    15061 tcgtattgat cttagcagct ttattacttt atatcttagt tcgaggaata ccgcacattt
    15121 catggtcatt cttgactgaa ccttccaaag cctaccaaga aggcggtggg atcgggattc
    15181 aattatttaa ctcgatttat ttgcttttga ttaccatgat catcagtttt cctatctctt
    15241 taggtgcggg tatctacctt tcggaatatg ccggaaaaaa ctggctgaca gacgtgatcc
    15301 gtacgtctat cgaaatcttg agctctttgc cttctgttgt tgtcggttta ttcggtttct
    15361 tggttttcgt tattcaattc cagtatggtt tctcaatcat ttctggtgca ttagctttga
    15421 ccgtattcaa cttgcctctt ttgactcgaa cggtcgagga atctttacaa gcggtccatt
    15481 acacacaacg agaagctgga ttggcgttag gtttgtcacg ctgggaaaca gtgataaaag
    15541 tggttgttcc agaagcgaca ccaggaattt tgactggtgt gatcctaagt tcaggaagaa
    15601 tttttggtga agctgccgca ttgatttata cagctggtca aagcgcgcca gcattagatt
    15661 ttacgaactg gaatccatta agtgtttcta gtccgatcag cattttccgc caagctgaaa
    15721 cactggctgt ccatatttgg aaaatcaaca cagaaggtac gatgcctgat ggtgtttctg
    15781 tttcggctgg cgcttctgcc gtgttgatca ttgctgtttt actgttcaac ttcggcgctc
    15841 gtgcgattgg caaacgatta taccgaaaaa tgacttcagc ctaaggagaa gaagtaaaat
    15901 gaaaaaatat aattggaatg aaacaaatgt cattaccatg ccaaaagata aggaagttgc
    15961 attatatacg aatgatctgc atgtttggta tggcgacaat gaagcaatca agggagtgga
    16021 tctagaattc gaaaaaaata agatcactgc attgattggt ccctctggct gcggaaaatc
    16081 cacgtatctt cgttctttga atcgtatgaa tgatgggatt gctaatacga aaattactgg
    16141 aaagattatg tataaagacg tggatatcaa tgcgcctcaa gtggatgttt atgaaatgcg
    16201 taaacggatc ggcatggtgt tccaacgccc caaccctttt agcaaatcga tttatgacaa
    16261 tataactttt gctttgaaac agcatggtga aaaaaacaaa aagaaattag atgaaatcgt
    16321 tgaaacaagc ttgaaacaag cagctttgtg ggatcaagta aaagatagct tggataaaag
    16381 tgccttggct ttatctggcg gacaacagca acgtttgtgt atcgctcgtg caatcgcgat
    16441 gaaacccgat atcttgctgt tagacgagcc ggcaagtgcc ttggatccta tttcgacagg
    16501 aacggttgaa gaaacacttg taaacttgaa agaagactac acgatcatta tcgtgacgca
    16561 taatatgcaa caagcagctc gtatcagcga ctatactgcc tttttctatc taggaaaagt
    16621 aatggaatat gacaaaacaa acaagatatt taccagacca aaaattcagg caaccgaaga
    16681 ttatgtgtcc ggtcattttg gttaagacgg aggcctcaac atgacaaaag aaattatctc
    16741 atccaaagat cttcatctgt attatggaaa gaaagaagca ctaaagggaa ttgatctaag
    16801 tttcaatcaa ggtgaaatca cagcgatgat cggtccttct ggttgcggga aatctaccta
    16861 tctacgttcg ttgaatcgaa tgaacgatct gattccaaat gtaacgatca ccggaagtgt
    16921 tttatataaa gacaaagaca tctatggtcc gaaaacagat gttgttgaac taagaaaaga
    16981 aatcggcatg gtgttccaac agccaaatcc atttcctttt tctatttatg aaaatgtcat
    17041 ctatggattg aagttaaaag gtgaaaaaga taaaaaagtc cttgatcaag tagtggaaga
    17101 aagtctgaag gcagcgtccg tttgggagga tgtgaaagat aaactgcata aaagtgcctt
    17161 gtctttatcc ggtgggcagc agcagcgtgt atgtatcgca cgtgttttgg cagtgaatcc
    17221 cgaaatcatc ttattggacg aaccaacaag tgcacttgac cctgtttcta ctgggaaaat
    17281 cgaaagtatg ctgttagaat taagagagcg gtatacgatg atcatcgtga cccataatat
    17341 gtctcaagct tcacgtattt cagataagac ggcattcttt ttagatggtc acttgatcga
    17401 atacaacgat acgaagaaga tattcatgaa tccagaaaaa caagagacag aagattatat
    17461 ttcaggtcga tttggataag gaggaaacgg aatgctacgt acacaatttg aagaagaatt
    17521 gctgaatctc cataatcagt tctacgaaat ggggatgatg gtgagcagcg ctgtgcacaa
    17581 gtcagttcgt gcatacatca aacacgataa aatcttagct caagaagtta tcgaaaacga
    17641 tataaatatc aataatatgg aaacgcgttt ggagaagaaa agttttgaaa tgattgcatt
    17701 acaacagcca gttacaactg acttgcgtat gattatcacg gtcatgaaag ctagctctga
    17761 tttagaacgt atggccgatc atgcggtgtc cgttgctaaa tccacaatcc gcgtaaaagg
    17821 acagacgaga ataccggaaa tcgaaaaaga gatctccgat atgtccgatt acgtgaaaaa
    17881 aatggtggac aacgtcctag ttgcgtatgt caaaacagac gaaaaagatg cgcgtacaat
    17941 cgccaatatg gatcaaagag tcaatgacta tttcaatcga atctataatg caaccatcaa
    18001 aaacatgcaa gaaaatcctg aaacagtcat cagtggcaca gactatttga atgttgccag
    18061 ctatctagaa cgaatcggag actatgtgac aaacatctgt gaatggatcg tctacttggc
    18121 tacagggaaa atcactgaat taaatacgaa ccacaatgag attttctaaa ctgaagttgt
    18181 gattctgctg tttatttagg aaaagaggtt gtgaaatcag cgttttgtcc tgagtattag
    18241 agaaacaaac ggtaaaatag ttcctcatat tttgccgttt tttgagctaa taccgaaggt
    18301 cagctgattg aacaccgttt attctggaaa agggtttgtg acaaaacatg tcacaaaccc
    18361 ttttcattat tccaatagaa aggataaaga atgcaaaaaa agattagcat catcatcact
    18421 ctatttttta tttgtatact tgcttcatgg tttattcagc gtcaagctgc tctatatgac
    18481 gtacctgtca tcgagatcga aagagtgaga atagaagagg atcaacaatt gattacagga
    18541 agattgatga atacggacca aaaaggagaa gtgatttctt taacggctcc ttatcaaaaa
    18601 aatcaaatcg aaacagcaaa tataacaaca agacaaatct atcttgtcca aatgcatgga
    18661 gatgaatggc aactagtagc aaaaaaaaat gatggctggc tttttttctt tacaagtgtc
    18721 ttcattggca ccatgttgct tattggtgga aaacagggcg tatttgcttt aggaagtctc
    18781 atcatcaaca tcgttttact tttacttttg ttgctcctct ttaccaaaac agaaggtatt
    18841 tctctcttaa atatagcaat cttatttgtc gttataggga taatcgttag tatattggcg
    18901 ttagacggat ggaatagaag cagtataatc aaaatttcgg ctgcagttac aagtgttttt
    18961 ttagcatttt ttatttgtct tttgacaatg aatcattgga aagatcaagg actgcgctat
    19021 gaagaactga attatctgac acgaccgtat cgttctgttt ttctttccag cgtgctgatt
    19081 gggacagctg gagggacact cgataccgtg atcacagtca ttgcgacatt agaagaattg
    19141 accaagcaac aaccgaaaat atcagcgaaa cagttatggc tttcaggaag gcaagtgggc
    19201 cgagatgtga ttggttcgat ggctaatatc cttttattcg tctatatcag cggagcgatt
    19261 ccatcgcttc tgttatattt aggaaatcaa tggtctttca aagaaacggt agaacgacat
    19321 ttatcattag aacttttacg cgtcattgct ggcagcttag gaatagtatt atcaatcccc
    19381 atcgcggtcc tcttttttct cgctgtaagg aggttaaaaa aatgaatgta cttattatac
    19441 ttgttgggat ttttgcaatc agtgtgctat ttgtcggtgg tacgcaaggt atgtatattc
    19501 ttttaggatt attcattaat ttaggaatct ttttcctatt attattcggc tatcatcaga
    19561 agtggccgat attggtattg tcgattatag ggttcctatt gatcgctgtg gttatcttat
    19621 tttttatcaa tggttataat ctgaaaatgc gagccgcttt tgccagtata ctgatctttt
    19681 tgttttgttt cctactattg attcctatca ctgatttttt agcgatccaa ggctttactt
    19741 cgatagaact agaagaacta tctggcttag ataagacgtt agcaattgat tttagactac
    19801 ttacgaggtc gttgcttctg atcagtctta gcggagctgt tttagatgcg agtgtagcga
    19861 tttctagtgg aacattcgaa gtatatcagg caaatccgca aatgtctttt aaccaattgc
    19921 gccatgctag ctttgccatt gctaaaaaaa tattggcatc aacagtgatg acgcttcttt
    19981 ttgcgtttat ggggagttct ttagcgttga ttctttggtt tattgactta gacatttctt
    20041 ttcagcaaat aatcaatgaa aaatcatttg tcttggaata tacgatggct attcttacca
    20101 cactggccgc actacttgtc ctgcccttaa cttgcgtgac tacagcatat ctctttacaa
    20161 aaaagaaaga acatctcaag atggagtaaa agttacaact taagtcctat gacattttta
    20221 ctgaatcagt gcataatatg gatgtaagga aaagcttgca aacaattttg cttaaggagg
    20281 caatttccat gaaagaaaga gaacgtattt tagacttagt gaaaaaagga atcctttcga
    20341 ctgaagaagg cttagattta ttagaaagca tggcaactga aaaagacgaa aaacaaatca
    20401 aaaaagaagc agacaaggta actgctagtc ataaagaaaa agatcaggca agtcaattga
    20461 tcgatgaact tgaaaacggt gaagaaaaaa tttctgagcc agttgatcca aaagaaaaag
    20521 aaagacaaga tcaagaaaac ttagaaaaga tcttagatga actagcaact gaagcgaata
    20581 aaacttcggc gcgtctagac gaagtaaatg ctcaattcgc tgacaataag gcagcacgta
    20641 atgagaaaca ggaagcattg atgcagctga atacaaaaga agaattaggc gagttgacag
    20701 aagaagaatt agcacagcgt caaacgttag aagcagaaat caaagagtta gaagcaaccg
    20761 gcgatacact attagaagaa caattgaagc tggaagcaga attaaaagat atccgcaaga
    20821 atcaatggtc tgagaaaaaa gaaacattta cggataagtt tgaattgcca gatgattgga
    20881 aagatcaagc gacagatacg ctgaatcaag taggcgaaaa aatgtcagaa gctggatcac
    20941 agttaggcaa attcttgaag aaaacattcc aaactgtttc agaaacagta aatgataaca
    21001 tggaatggaa agatgtcagc ttacgcgtcc ctggaattgc gacaacgaaa tttgaacacg
    21061 aattttatta tgaagctcct gcagcaagta tccttgatat caaggcagca aacggaaacg
    21121 tgacgttaaa aacgtgggac agtgatgatg tgaaagtgga agccaagatc aaactttacg
    21181 gaaaaatggg tgctgaacca tttgaagcat tctctgaacg gagccagatc gaggtcaatg
    21241 aagatcatat ctccttccaa attccaaata agcgtgtgcg tgcagacctt gttttctact
    21301 tgcctaaacg agtatacgac catgcagcta tcaaacttct aaatggcaac atcatgattg
    21361 agacattgga agctaaagat atttatacaa aatctacgaa tgggaacatc atcgtgaacc
    21421 aattaactgc aacgatgctg gaagtagaag gtgtaaacgg caatatcgat attcgaaacg
    21481 ggaacatctt agattctatt atagaaacag taaacggtac agtgacattt ggtgccacac
    21541 cagaaaacct ttctgtgtct ttagtaaatg gtgatgtacg tcttacgatt aaagaagata
    21601 atttgaaaaa agtagaagca agttcagtga acgggaatgt caaagttgct ttaccagata
    21661 gcatcggttt ggaaggacac gctaaaacaa gtcttggaag cattaacagc cgtctgtcca
    21721 attacgaagt agttcgtgag aaaaaagaac ggacaaacca aatgttgcaa ttccgtcgtg
    21781 tttcagatga tgagatcgca caagtgcagc tttcaacgac aacaggaagt atctatttaa
    21841 aagatacaga taaataaaga ggttgtgaaa gagatgttca gccttgagca ataagaagcg
    21901 attttttaaa atagcttccc ctattttaaa aaaatgtgcc ttattgccga taggctatct
    21961 cttgaacacc gattaagaag aaggttgtca caacgatttt cttcatttaa aatagtagaa
    22021 cagaggtgag acatgatgaa aaaaaagttg acaaaatcca ataaaaatgt tgttttgaca
    22081 ggtactttag cagggattgg ggaatatatc gggattgatc ccactgttat ccgtgtgctt
    22141 tatgtattct taagcttagt tgcttttgga agtccaatca ttttatacat tttattagct
    22201 ttgattatcc catctgcgcg ttctgcacga acaaactatg gacataacaa tgcatattat
    22261 gaaaaaaaca attatcgtga acaaacaaaa aaacgcaaag aagcggaaaa aatcgatgat
    22321 gacgaatgga gtgactttta atgtcgtact ttcagcgtat tttggttaat acgttaacat
    22381 tcatatcgtt ggctgttctt tttcctgcga tggtccatgt gagcagtatt tggacggccg
    22441 tccttgccag cttcgtatta tctgtgttga acatgtttgt caaaccgatt ttgacgatcc
    22501 tgtccctgcc ctttacatta gtcacattgg gattgtttac gtttgttatc aatgcagcga
    22561 ttcttaaaat gacttccttt tttgtaggag cagaacattt tagtttttct tcatttggtg
    22621 cagcgttatt cgtagctatc ttgatgtcac ttgtcaatgt aattgttagt gaacataaca
    22681 gcagaaaata aaaaaagctg tgccgagatc tcggcgcagc ttttttgttt agaataaaga
    22741 atgcgttttc tattacattg gtaattaaat aaaaacgttt tttttaattg gtagtacttt
    22801 tggctagaca atgataaaat gatagagaac actctaatag aaatgaggta gcatgaatgt
    22861 cagaagttgt aaaagttagc caattagtaa aaaaattatc tttggaaatc gtcacaggcg
    22921 acgagaaaag tttagaccgc gtcatcgtga ctggggacat ctcacgccca ggactagagt
    22981 tgaccggtta ttttaattat tattcccata atcgtttaca gctatttggc agtaaagaaa
    23041 tcagttttgc ggaacggatg atcccagaag agcgtttgat cattatgaga agattatgta
    23101 gtgaagatat gcccgcattt attatttcaa gaggattgcc ggctccggaa gaaatgatcc
    23161 aagcagccaa tgaaaatggg atagctgtgc tgcgttcgcc tatctcaacg tcccggctgc
    23221 taggtgaatt atctagttat ttagatggac gcttagcccc tcgaacaagt gtccacggtg
    23281 tgttagtcga tgtctacgga ttaggtgtac taattcaagg ggacagtggg atagggaaaa
    23341 gtgaaacggc gttggaactg attaagcgcg gacatcgatt gatcgcagac gatcgagtag
    23401 atgtctatca gcaagatgaa ctgacggtaa tcggagaacc tcctaaaatc ctggaacatc
    23461 tgattgagat tcgagggatc ggtatcatag atgtaatgaa tcttttcggt gcgagtgcgg
    23521 tacgaggctc tatgcaagtc cagttagctg tttatctaga agcatgggcg aaagataaga
    23581 aatacgaccg tctgggctct gaagacacga ccgttgaaat cgcggaagtg ggcattccac
    23641 aaataaaaat ccctgtcaaa acaggacgaa acgttgcaat catcattgaa gtagccgcaa
    23701 tgaatttccg cgcacgtaca atgggctttg acgcaacgaa aacatttgaa gaaagattaa
    23761 gtcgcttgat tgaagaaaac tcaggcaatg actagcaagg agtgacaata tgttagcaca
    23821 aatcaatcga gttgcttttg agcttgtagg aattcctatc tactggtatg cattgattat
    23881 cgtatcagga atcattattg ccatgtggct cagcagcaga gaagctgttc gggtaggatt
    23941 aaaagcagat gacgtgacag attttatgtt gtggggattg ccgatttcca ttattggtgc
    24001 ccgcttgtat tatgttttat tcgatcttcc ccaatatata gctgatccaa tccagatatt
    24061 caatatccgt tcaggaggat tagcgattta cgggggattg attgcaggtg caatcacact
    24121 gtactttttt accaaatatc attttatttc tttgtggacc tttttagata tcgctgcacc
    24181 aagcgtatta ttggcgcagg caatcggacg atggggaaat ttcatgaacc acgaagcgta
    24241 tggtccagac acgacacgaa gtttcttaga aagtctgcat ttaccgcaat tcatcattga
    24301 taacatgtat atcgaaggaa tctatcgaca accaaccttt ctttatgaat cactttggag
    24361 tttgattgga tttattctgc ttttggtttt gcggaagaaa ccacatcttt tgaaaaaagg
    24421 agaagtcctt ttatgttatc taatgtggta tagtttcgga cgtttcttta tcgaagggtt
    24481 acgcacagac agcctttact ttttaggtgt gatccgcatt tctcaattat tgtctgctgt
    24541 tttatttgtc ggcacattga ttctgtttat ttggcgtaga aaaaaaggaa gtttaccgga
    24601 ttatgaccgc tcattaggaa aaaatcaaca aattatttga ttttcagagc gttaaaaatg
    24661 aaaaaaattt caggagtggt aaaatgaatt atagagtagc ggttttagga ccaggctctt
    24721 gggggactgc tttagcacaa gtcctcgctg aaaacggaca tgacgttcgg atttggggac
    24781 atcgtgcttc acaagtagaa gaaatcaaca cccaacacac caatcaacgt tatttaccag
    24841 atcatcatct acccgattcc atccgagcat tcaccgatat ggaagatgca gtaaaagatg
    24901 cagatgcggt actattcgtt atcccgacga aagcgatacg ttctgttgca aaagaactag
    24961 ttgccaaatt gaatacgaag ccagtgatca tccacgcaag taagggatta gaacagggaa
    25021 cgcataaaag aatctctgaa atcttgttag aggaaatccc gacagaaaaa agaaaagctg
    25081 ttgctgttct ttctgggcca agccatgcgg aagaagtagc aaagcatgac attacgacga
    25141 tcacagcggc aagcacaaat gtaaaagcag caaaatatgt acaagagctg ttcatgaacc
    25201 attattttcg aatctatacc aaccctgatg tgattggtgt agagatggga gcagctttga
    25261 aaaatattat tgccattgga gctggtgcta ttcacggctt agggtttggc gataatgcaa
    25321 aagcggcaat catgacgcgc ggattagctg agatcagccg tttaggtgta gcaatgggtg
    25381 ccaacccgct gacctttatt ggcttaagcg gagtaggtga tctagtcgtt acttgcacaa
    25441 gtgttcattc cagaaactgg cgcgcaggca acttgttagg acaaggtcag cctctagaag
    25501 aggtgctagc caatatggga atggttgttg aaggtgtgaa tactactcaa gcagcgatgg
    25561 aactagccca aatgatggaa atcgaaatgc ccatcactca aacaatctat gaagtgctgt
    25621 atgaacaaaa agaaatcaaa acagccgcaa aagaaatcat gttacgtgat gggaaaaaag
    25681 aaaatgaatt ctcattaaca attttttgaa ggagcgaatg tatatgaaag taaaaaaagc
    25741 agttattcca gcagcaggat taggaacaag atttttacca gcaacaaagg cgatggcaaa
    25801 ggaaatgttg ccaatcgtgg acaaaccaac gatccaattc atcgttgaag aagcgttgaa
    25861 atcaggaatc gaagacatct tgatcgttac aggaaaagct aaacgaccaa tcgaagatca
    25921 ctttgattca aatatcgaat tagaaacaaa cctaagtgaa aaaggaaaaa ccgaattatt
    25981 gaaattagta gaagaaacga cagatgtcaa tttgcatttc atccgtcaat ctcatccaaa
    26041 ggggttagga cacgccgttc ttcaagcaag agcctttatt ggaaatgaac cgtttgtcgt
    26101 tatgctaggc gatgatatca tggaagacga agtaccattg acacagcaat tgatcgatga
    26161 ctatgagcgt acacacgctt ctacgattgc cgttatgcaa gttcctcacg atgaaacttc
    26221 aaaatacgga atcatcaacc caggggaagt actggaagat gggttataca atgtcaaaaa
    26281 ctttgttgaa aaaccagatc caagcaaagc accaagtgat ttagctatta ttgggcgtta
    26341 cttattgact ccagaaatct ttgatgtatt atcagaacaa caaccaggcg ctggaaatga
    26401 gatccaacta actgacgcaa tcgatacgtt gaataaaaca caacgcgtat ttgcccgtaa
    26461 attcacaggt aaacgttatg acgttggtga caaatttggt tttatgaaaa caagtattga
    26521 atatggtctg acacatccag aagtaggggc acctttgcgt gaatacatca tgtctttagg
    26581 taaaacatta gaagcagaag gaagcaagat caatcataat ccaaaaggaa taacagaaat
    26641 caaagaagaa aagaaaaaat aatctttttt tctaacgagc ttcagctgct aattgaataa
    26701 aaagtagttg aagctcgttt ttatttgttt aaaaaacacg aaaatgctat ccttatagaa
    26761 aagagagaca agaagggagt ttgatcatgt caggaggaga aattgcagga ttgatcgcag
    26821 caatcgcctt tgcgatactt gtcgttttca tcgttcgcgt attgttgcaa gtttccaaaa
    26881 ctttagaaaa agtagaccag actgtcgcag aagcaaatac gacaattgag atcgtcacac
    26941 aagacgtaga tcttttatct agacaagtag aaggcttgct agtcaaaagt aatgagctgc
    27001 tcttggatat caatggaaaa gtcgcaacga tcgatccatt gttcacagca gttgctgatt
    27061 taagtgaaag tgtatcggaa cttaatcatt ctagtaaaaa catcgtgacg aaagtcggcg
    27121 gaatcggaaa aacgacagct aaagcgactg ttgccggaaa agtcggtgga acagcgatga
    27181 aatttttaaa aacaaaaaac atactgaaac gacaggagga aaataagaat ggctaaaaaa
    27241 ggtggatttt tattaggtgc agtagttgga ggtacagccg cagcaatcgc cgcattattg
    27301 ttagcaccca aatctggtaa agaacttcgg gatgaattgg ctgctcaagc agatgatttg
    27361 atggacatgg cttcagatta cacagatatg gcaaaagaaa aatcatctga gttaacaggt
    27421 attgcaaaag aaaaagcgca agatctttct caacaagcag gggatctagc aggcaatgtt
    27481 aagcagaagg taagtgaatc agcagaggaa gcaggaagcg aatcagatga tatcttgtta
    27541 aacctcaaag aacaatcagc tgatttatct aatcgtttca aaaaaacagc agaagatctg
    27601 aaaaagccag cagacgaatt aggtagtatt gctaaagata cttcagaaga tatcatgatc
    27661 gatgtaaaag atgctgcaga taaagcagga gagacggtca atgaaggaat gtcagaagca
    27721 aaaccagttg cagatgacac aaaagaaatg ttgaaaatgg caaaagaaga tgcaaaagaa
    27781 gatgcaaaag aagctaaaaa agaaatgaaa gatacaatga cagattcgac taccaatcac
    27841 taagattact catttctaga ataaaaaagg tgagagcgga ccagctctca ccttttttat
    27901 tttcattact ctaaaacaag taattctttt ggtgtagtcg tgaatggcac acaaccatca
    27961 cttgtcacat acacacaatc ttcgatacgt acccctactt tttcaggaat atagattcct
    28021 ggttcaagag agaagcacat cccttcttca atcactaagt cgtttccttc tacaagagaa
    28081 gggaattcgt ggacggttgt tccgattccg tgacctaaac ggtgattgaa atattcgccg
    28141 tagccatagc tgttgatcac gtttcgtgcg atttgatcaa gttcacctgc tgtcacacct
    28201 gggcgaactg cctcttgtgc ggcaagctgt gcttcaagag tgatgttata gattttttct
    28261 tggaattcag ttggtttttg ataagcaact gtacgagtag catcactgca atagccattc
    28321 caaataacgc caagatcgaa taacactagt tcatttggtg aaaccttcgt gcttcctggt
    28381 gtaccatgag ggcttgcagc atttgctcct gcaaggacta atgtgtcgaa agacatttgt
    28441 gaaacacctt gtttttttaa ttggtattcg atttctgcga taattgcttg ttcagttacg
    28501 ccttctttta ctgttttgaa gccaatttca aaggcaacat cggcccattt accagcttcg
    28561 atcagtttgt ctttttcttc taccgttttg atcagttgca gacgctggat aatagaagtt
    28621 agatcggtgt aagaatctgt acctgtcaag atatcagaga tagcatcgaa gcgatcgaca
    28681 gtcagagcat tcttttcaat acctacatta ccaggttgcc catagcgtgc gcgaagctct
    28741 tttccgatga tctcccaagg attttcgctg tctaagtagc cacgtacatc gtacgcccag
    28801 ctgctgtttt gagcatcttc aacttccaga gcaggagtga aaaggaaagg atcttgttcc
    28861 agagggataa aaagagctag tacacgctcg tgaggatcac tttcatatcc ggaaaagtaa
    28921 gcgatgtggc cagggtcgct gatataagcc aattctaatt ggtgttgtgc catccattga
    28981 cgtagttcat tgatttttac gttattcatt tggcaaccta ctttctaaat taattttgta
    29041 ttcaataatt gtaccactga ttgattaaaa gaccaacgtt cttaaaactg aaaaaagtaa
    29101 aaattttcat gaaaacagag ataaaacggt tgcaattgtt gagattattt gatatcctat
    29161 tttagaaatg taagacgtat tttcagaaaa catataaaat atgaatagga gaacacaaat
    29221 ggaaaagcaa acaatcacta tctatgatgt agcgagagag gcaaatgttt cgatggctac
    29281 tgtttcacgt gtcgtaaatg gcaatccaaa cgtcaaacca gcaacaagaa aaaaagtctt
    29341 ggaagttatc gatcgcttgg attatcgtcc aaatgcagtc gcacgtggct tggcaagtaa
    29401 gaaaacaaca acagttgggg taatcattcc tgatgtcagc aatatgttct ttgcctcttt
    29461 agcacgtggt atcgacgatg tggcaacaat gtacaaatac aatatcatct tagcaaactc
    29521 agatggcaac gatcaaaaag aagtcaatgt attgaataat ttattggcta agcaagtgga
    29581 cggcgtgatc tttatgggtc accatatcac agacgaaatc cgtggagaat tttcacgttc
    29641 aaaaacgcct gttgtactag ctggttccat tgatccagat gaacaagtag gtagcgtaaa
    29701 cattgattat gtatcagcaa caaaagatgc agtaaataaa ttagcaaaaa gcggcaataa
    29761 aaaaatcgct tttgtcagcg gtgcgctgat tgatcctatc aatggtcaaa accgtctaaa
    29821 aggctataaa gaagcattaa gcgaaaacaa tctaccatat agcgaaggat tgatttttga
    29881 ggcacaatac aactttaaag atggtctgtc tctagttgac cggatccata atagcggtgc
    29941 aacagctgtc tatgtttcag acgatgaatt agccattggt atcttggatg gtttattaga
    30001 ccgcggcgta aaagtaccag aagaattcga aatcatcaca agcaacaatt ctttactgac
    30061 agaagtagct cgtcctcgtt tgacaagtat cacacaacca ttatacgatt taggagcggt
    30121 atccatgcgc ctattgacga aaatgatgaa taaagaagaa gtcgaagaaa aaacgattat
    30181 cttgccttat ggaatcgaag aaaaaggatc aactaaataa taaaagatca atcatttaaa
    30241 ggttgttccc aaagtggtca acttctggaa caacctttat acgagaaaag gggttatgac
    30301 aaaattttgt cataacccct ttttttgtga ctttaaaaaa aagagagaat ggcggaaaca
    30361 aaccttacgt ccatcgtata cgacacaatc tgtgcgtaga tgctacattt tgttcactac
    30421 tatcaaaatt actttaaaat gaaaacgtga taaaaaagaa agtagaaaag tagaaaagta
    30481 actctaaaaa atccaaaaaa atccaggctc gattgcgtac ttatcttaaa ggagaaacaa
    30541 tccatcatac ataacccgaa aggagacttc tatttgccta agaaaagacg aaagaccata
    30601 gtcatcattt tgaccattct tactatagga atcggaggaa aattttacat ggacaaaaga
    30661 gaagcgcaaa aacagcaaga tttactcgca gtagaaaaac agtccgtcaa agttctgaaa
    30721 aatacctttg cggatatcaa agaagtgaaa atcgaacaat ttgagaaaaa cgacatgaca
    30781 ggaatctatg aagcattgat aacgatgacc aatacagaag gaaaagccgt ttattttgat
    30841 tatagcttta caaaagaaag caatgagata gatggctatg gtgttgaaaa tgaagaagta
    30901 caaaaagaag ggattacggt tgatgagatt atagttatct atacagatgg gagtgaggaa
    30961 aaagtatgac aattactgat aagcagtata aaggaataag taataaagta tattggctag
    31021 atccaaaaca tcctcaatat agtaaaagtt acaaagaagg cgccattaaa aactttggag
    31081 aaacaaaatt ccaaatccta aaaatcaaaa actccttcga cggcatgcag gcgatggctg
    31141 tagcgccaat caagaacggt aaagtggata tttctcaagt agtgattgct tttgcaggaa
    31201 cgaacttaag cgattggaaa gatttggaaa cggatgcaag aagtgttatg aatctctatg
    31261 ggaatagacc aggagcaagt ttttctgaaa aagcaacaac aaacaagatt ttatccgtta
    31321 gtggattgaa aacagcgggg caaatcaatt cagcgattgc atttgctaat gaagtaaaaa
    31381 aggaatatgg agaagctgct atttctacta ccggccattc attaggtgga ttcttagcat
    31441 tatatgtagc agcagagaaa caatggaaaa atgtcggctt caacgggccg gacccgtatg
    31501 atctcttatc accagaagca aaaaaatggg tggaagaaaa tcccggtatg ctcaccaatt
    31561 accgaaatca cgccgatttt atcggtaatt ttggtggtaa tggcacaggt gctgaagttc
    31621 ggatcagtat ggaaacagca tacttgaatc tgcttgcttc ccacagttta acctcttgga
    31681 agtttgatga aaatggaaag ctgattattc ctgaaaatga aaacaatgtt aaagctcgaa
    31741 aacaacagaa agaaaaccaa gcgacccagt tgttttccta tcagttgcaa gaactgaaca
    31801 acatacgtaa aaaactacga ttaagtggcg gccggctgac agttaatgaa caaatttact
    31861 tagatgacac acaagctagg ttagtggtgg aaaagttatc ggtaaaaatg aaaagcgcta
    31921 tggaagaagt tatccgcatc aaccaaagag gaatttttga gctagagaaa aaatggcaag
    31981 aaggcttgcg gcaagcacgt gttgtcgccc cttcgttgac tgaacaggaa atcatagaag
    32041 cattagctag tgtgggcgcc acaaaagaga acttagtcga ttctgcgaaa gaagtctatc
    32101 tgctacgaat caataaggca agacgtatgg gggaggcctt tgatacattg gcgaaggaga
    32161 tccaagacaa aatcaatgaa atcgtggcac gagatcgaga attagcacag caattcaatt
    32221 aaaggagaag tagatgaaac tagaagaatt ggaaagagat tatcgaagtg aacgaagaca
    32281 attagaagct caagaagatg aattgatcca ctttaagaat caaggattac gattgatgga
    32341 gcaagaggag cagcgactgc atcatgtatt aaagaactta gctttagata atgagcctct
    32401 gtttcaagca agacgaagtt attcgaatta tcaggagcat tatcaacaag ccctacaagt
    32461 agaacgacga aaactctatc agcagcaaga cgaactagaa cgttcctatc gtcaaaaaat
    32521 cagacaaata actatggaga aatcatctta tgaatgaatt tttaatgcaa ttactaaaag
    32581 aattagagaa aaatctacat cgatcaaatg tacgttcagt tggtatgggg atatctcagc
    32641 aaacttgggc agaaaaacga ttgacagatc aaaaccacca aatgactgca gaagaagcta
    32701 ctcaagaatt ttctaaccga catgcacact taaaagaatc agtaaaaggg caatttgcag
    32761 aaaaactaac cgaattattc caaagacaag aaaagaaatt aaaacagttt cgttagtaaa
    32821 gttatataga aaaattttgt gaaaatggtt tttcacattt ttacataaat ttgtcttaat
    32881 atcgtagatg taagcttttg aacaccattt attcggaaaa agggagctgt gacaagattt
    32941 ttgtcgcagc tccctttaat tctgattaat ctattagatc aatcatcttt atcgtctgat
    33001 tctttggtct ctggattcgc tctagcataa gctgaagcgg tgttccagta atctgtatag
    33061 ttttcatcag taccgccaac gccgaattta aaggcgcttt tcttcggacc attttttgcc
    33121 caaagtgatt ccactgtttt tcctggcgtt tggatagatt ttttattgac agtaactttt
    33181 ccagctgttt gtcctgtgaa cccagatact tttttcttca tcacatcgga tgataagtca
    33241 aatttctcat cagtgccgaa aatctcaggg tttgcttggt aagcttgatt gattagattg
    33301 gcaagataat tagccgaacg tattcttgct tggctgtcca ttccagtatt gtcatcgtgt
    33361 cctgcccaac tactgatagt gatagatgga gttgagacga tcagccatga atctcggtat
    33421 tcattggtag aaccagtttt tccgacccaa tctgcatttc ctagatttcc gtttaagctt
    33481 gagatgacat ctttgaatgg tgtagtgatt ttttcattga tcacactacg catcatgtca
    33541 ttcatgatcg atgccgttgc ttcggaataa atacggacag aattactttc atgtttataa
    33601 atcacattac ctgcattatc tgtgatggaa tcaatgatat agccttcttc gtagactcca
    33661 ccatttgcta aggcctggaa tccatttgtc tgttgaagcg tcgttacatc tactgtccct
    33721 aaaggagccg attcaacgcc ccaattatca tttgctgggt agttcatctt cgccaagtag
    33781 tgctcgtaag cgtattgttt cgatccaccg gaattcaaca catcttgata taagtgataa
    33841 gctgggatat tgttggacca ttccagtgac tcacggacgg tttggaaggt gttcgaccct
    33901 tcattggtcg cattgacgat tttctcacca gcattttttc cttcttgcca agtcgttgca
    33961 taatctgata cacgggattc tgatccgaca agtcccatat cgattgcagg accgtaaaca
    34021 agaaccggtt tgatggatga accagcttga cgttctgtat caaaggcgtg gttattttga
    34081 ttctggctgt aattccgacc acccacgaag ccatagatac gtccggttct gttatccatc
    34141 aatacatttc cagtctccac ttggctacca gcaccatcat ctaacatgta gccataattt
    34201 tctacgccgg tttgcaatgc agcatagata tctttatcaa tcgtcgaatg gatcgtatat
    34261 cctttgtttt ggatcgtttg ctgtgctttt tgataataag cttcactgac cgatgaatct
    34321 gacatatcag cattatcttt ttctgccaat tgttgtgcaa taatccttgt tgcttcgttc
    34381 atcacggtat aatacaagaa ttctctgtca gtctgttccg cgatttgttg tgggagaaag
    34441 tcttttgtaa gatcataggc tttcgcttct tcgtattgtt ctttcgtaat ttgtccttca
    34501 cggtacatac tgaataaaac aaaatctttt ctggcaagac cggcagaaag atcttctttt
    34561 agcgcgcctg tattcgtgta tggactatac gtgatagggc tttgaggcaa accagcgata
    34621 taagcagctt gcggcagagt cacatccttt gcatctacac caaagattcc tctagcagct
    34681 tcctggactc cagcaatatt ttgcccctta ttattccgac caaatggtga gacattcaaa
    34741 tacgtagtaa tgatctcatc ttttgaaaag tgtttttcta cttgtgcaga aagaacgatc
    34801 tcgtttgctt tacgtttgaa tgttgtttca tcggttagga tctgttgttt gactaattgc
    34861 tgtgtcaaag tggatcctcc agacgagccg atgccagtcg cttctgaaac tagtgctcgt
    34921 aaaacagctt tagggacata accttggtgt ttatcgaagt attcatcttc tgtactgata
    34981 atcgcggttt tcaataaagg cgaaatctta tcagatgaaa tagtcgtccg catcagatct
    35041 gtctggattt ttgatatctc tgtattatct gcataaacaa tctttgacgt ttccgtaatg
    35101 tttcctagtt ctgtctgcag ttctttttta gtcggcaatt gtgtgtcttc gacaagatag
    35161 gcaaagtagc cagcacctat tccgatacct agagctgcga acaacaaaag aagaacagta
    35221 ataaaaacag tcaatgattg aaacactcta aaaataatct taggaacaaa ccatttttct
    35281 ttttttcttc tattctgttt ttttcgagta tttttgcgtg gcaaaagggt cacctcaact
    35341 aatggtttta tttcgctaat tatagcaaat aataggacaa aaaaacagac aaggctgttt
    35401 ttgacaggaa tttctttttc gaatgagata aaacgtgagg aaatgatctt ttttatgaaa
    35461 gagttcttaa gcttttcttt agggaaagtt tcagttacta tggagaacag ggcaaagaaa
    35521 aacaaactat tttctttgta catcatctta tcaatataca ggaattgttc gaaacaattg
    35581 cctttcaagg aggaatccat ccatgttatt aggctcacac gtcagcatga gcggaaagaa
    35641 aatgctgtta ggcgcggcag aagaagcagc cagttacgga tcttcgacct ttatgatcta
    35701 tacaggcgct cctcaaaata ctagaagaaa agctatcgaa gagatgaata ttgatgccgg
    35761 aaaagcattt atggcagaaa acggactaac aaatatcgtc gttcatgcgc cttatatcat
    35821 taatttggga aatacgatca aaccagaaat gtttccattt gctgtacaat ttctgagaga
    35881 agagattgtc cgtgcggaag ctttaggtgc tactcaaatc acccttcacc caggcgctca
    35941 tgtaggagca ggggcagaag ctgggattgc tagaattatt gaggggttga atgaagtttt
    36001 gacaaaagaa cagacggcac agatcgcttt ggagacgatg gcagggaaag gtacggaaat
    36061 cggtcgaaca tttgaggaat tagcgcaaat catcgatgga gtaaaattga atgaaaaatt
    36121 atccgttact tttgatacat gtcatacgaa tgatgccgga tataatataa aagaagattt
    36181 ttcaggtgta atggaagagt ttgataaggt catcggatta gatcggctta aagtactgca
    36241 tatcaatgat tctaaaaatc ctcaaggttc tcataaagat cggcatgcca atatcggttt
    36301 tggaacaatt ggatttgaca cattgaacaa aatcgtccat gatccaatgt ttgacaacat
    36361 ttcgaaaata ttagaaaccc catatgtcgg taaagataaa aagaaagctt acgcaccata
    36421 tggaaaagaa atttccatgt tcaaagcgca agcattcgat cccgaagtgt tccctgagtt
    36481 attagcagaa gaagtcacac tttaagtttt ctacctgaca gcaacaaaaa aggattgggc
    36541 agtctgcctt gtcctttttt gtttaagaaa caaaggagtg ctttttgcat tcgttttcgc
    36601 catgctcctt ttgccccctc tttttaaatt cagacaaaca gggtatactg atagaaaagt
    36661 aaaaaacgaa aagtttcaca aacaagtagc aaaacgtgat tgaaaggacg aaaagatgct
    36721 gaacatttta aaccccttga tcgaccaact aaaaaagaaa catacatact ttattcttgg
    36781 catagccgta ttatttacaa ttattcggtt gtatttatat agtaaagcaa tctatggaac
    36841 agatatgtcg gcgacctatg atgatcagct gctgattcac tatgctcaaa atattttgga
    36901 tggtaaatgg ctaggggagt acacgactac gacactttca aaagggattt cctacagctt
    36961 gtttttagta cttgcacata aactgcatct ttcttatgca gttttattcg gcatgttcag
    37021 tattcttgcc agtatattgg ttgcactagc gtttaaacca atcgtgaaga acaagtatct
    37081 tcttttaagt atctattttt attttttgtt ttctccgatt agttttacac atgaatattc
    37141 cactcgaaca tatcgtaatg ctctcgtgat cccgactgtt gagatcatta tcgcttgttt
    37201 actagctatt tattaccgca aatataattc cagcaaaaaa ttgctgccgt ggtttttagg
    37261 gctagctgtt tcttttcctt tcttttggtt cattcgcgaa gattccatct ggcttcttcc
    37321 ttttactgta gtcgcgttag gcatcagttt cgttcagatc ttgctaggac aagacttttc
    37381 tttcaaagaa gcaaagaagt taaaagaacg accatttctc ctttcgaaac aagaatggct
    37441 gaaactttca ttgtttttac tccctctagt cggtttctcc atcgtctatc agtggatcga
    37501 atataaaaac gagacaacat atggtattgc agccatcaat gaccgttcga gtggcgaatt
    37561 tggacgtttg atgaaacagc tgatccgaat agatgacgaa acaaacttaa ataaagaaaa
    37621 cagtgagata tgggtctcgc atgctgcatt aaataaagct ttagacgcat ctcctacatt
    37681 tcatactgtt tctactcgta ttgacgaatt gtatacgact catgcgtgga cccaaggcgg
    37741 aaaagtaaaa gaattaccag gagatatcat tttttgggca ttgcgagatg tggtaagcga
    37801 atctggttat taccaaaaca atgcaaaaga aaccaatgat ttttggaaaa aagtcaatca
    37861 agaattagaa gaagcctatc aaaagggaac tatcaaaaag aaaaaagaat tctatctgat
    37921 gggatcaggg gatggaaaga cgatgaatga tctgccaatc gtatttgatt ttctgaaatc
    37981 aggatatgct tatgcatggc gatacaaaga tttccatcaa ggggggaatt atagttttgg
    38041 gacgcaagaa caagtcgaac aggcacaaga attattgaat cttccactaa tggataattg
    38101 gatcgatagc agtgatccga gaacacctga tttccagttg acaaaaacag caaaagtcgc
    38161 gaatgtcatg ataaaaattt accaagttct ggccattccc gtgctgattg cttcaatagc
    38221 agggatatta cttatgatac taggaaccgt atttgcaaaa gctgatcgtg cctattatag
    38281 ttcctctttg attgtagtca tcggattaat actgacacaa ttggtttttc ttttcggtgt
    38341 ctcgtggttt tgttcttatg cgccagaaaa aaaggattat tttttaagtg tctacactgg
    38401 cgcaggcgtg ccattaacgc aattcatcgc agtaacaagc atgctagttt ttagtaaatt
    38461 gaatctgaaa aaagctttat cttccaaata aaaagggaac tgtgacaaaa gtcttgtcgc
    38521 agttcctttt tccgaataaa cggtgctcaa aagcttgcat ctgcggtaat aagctcaacc
    38581 aaacgtaaaa tatgaggaac tatttttgtt tggctgttct tattacttga tgcagggcag
    38641 ctttttcaca acctctgata cacggtgttc cagaagtaac ttcttcggaa ataagccgaa
    38701 atttcacaaa aatttgaaaa gcaattttct taaattcctt cttatttctt gaagctgccc
    38761 acttctgtca caacctcctt tgtgtaaaag aatcagtttc tattcgattt cttctatcgt
    38821 ttcttgcaaa cgctgttcat attttttgtt tcctttatat aaatcaccaa tcgtccatgc
    38881 caacaggaga aggacaagaa caatgattac ataggcgatt tgatcagcca atgctagttg
    38941 agctgaggtg gcttgctcgc cgaagaaact ttcgatttga cctggtaatc ctcgtaagtt
    39001 caagtaagtc agactaatca cagagaacca gccaagaagt tttacaacaa atgaattttt
    39061 gaatcggctc cccatctccg cttggctatt ggtcatcatc agtaaaggaa tcatcgagaa
    39121 tggcaatgca aaagccagaa aaacttggga ttcattcatc aagttattca aagcggtatg
    39181 ttcttcgatc gttccttttg tacttgtaaa caaaacacaa atcaatacag gaatcacaga
    39241 aagcaatcgt gtgactagtc ttcttagcca aatcgggata cgcatatgga tgaatccttc
    39301 catgatgact tgaccggtca acgtaccagt aatagtagag ttctggccag aagcaagaag
    39361 tgctacagca aataaagtag acaagatacc agattttgct acagcaatca agataccatt
    39421 gctcatagct gatggatcag ataaagcttc atacagacca aaaaaggaag gatcttggac
    39481 agaaccggtt ttgaatacag cgacacccat gatcaatagc agtgcattga ctaaaaaagc
    39541 cattgacaac tggatattag agtcccaagt agaaaagcgt accgcttgtg ctacatcttc
    39601 ttcgtcttgg tgatcgatct tccgagtttg agaaactgct gaatgaagat ataagttatg
    39661 cggcatgact gttgcaccaa taatccctaa cgctccagtc agcggagttt caccacctat
    39721 gacaggtgag tctgagaatg tatgactgtt agggataaag ccagcaaata aactgtgcca
    39781 atcagggtta gacaagccaa cttgatagac gaaaacgaat aaaatgacga aaatcaagca
    39841 gacaaccaaa gcttcgattt tacgaaaccc gacttttgtc agcaataaaa ggacgaaaac
    39901 atctaacaca gtgataaata cggcaattac caatggaata tcaaaaagta agtagagggc
    39961 gattgctgca ccaatgactt ctgcgatgtc tgttgccata atggctaatt cagtcaagat
    40021 ccataaaatg acacccaatg tcttacttgt ccgtgctcgg attgcttggg ccaaatccat
    40081 ttgggtcacg attcctaatt ttgctgccat atactgtaaa agcatagcaa ttagactaga
    40141 catcaaaata actgacatca ataaatattg gaaattttgt ccaccagtga tcgaagtgga
    40201 ccagttacct ggatccatat aaccaactgc tactaatgca cctggtccag agtaagcaag
    40261 aagtgtttta aaaaaacttt ttccctttgg tacatccact gttccattta cttcagctaa
    40321 agataaacca tttgtatgtt caataagtcg atgcttatga aattgtttgt tggattgatt
    40381 attcaagaaa aaaccacctt tcctatttat ctacgattac attctacacc tcagaaagaa
    40441 aaaattaaag aaaaagattc ggtatgccta attttttttc gtggcgtgtc ttgctggtta
    40501 atgaaagaaa ttttgttctt ctctaaaaaa accactattt tttgcatgaa actaaagaat
    40561 acgcctttct tttttcaata aactttagta aaataaatca ttgagagcaa ttcggaaata
    40621 gaaaggagag atttatatgg tatcaacagg tattgtcgta cttattgcta ttattgcttt
    40681 gctaatcggc gcagtaggtg gctttttcct agcgcgtaaa tatatgaaag actatttaga
    40741 aaaaaatcct cccgttaatg aggacatgtt gcgatcaatg atgatgtcaa tggggcaaaa
    40801 accatctgag aagaaaattc gccagatgat gcagcaaatg aagaaccaag ggaaaaaata
    40861 ggttctgacc caaagacttt ttgcgggctg attttttcag tccgtttttt atttcgtaat
    40921 ttgttagact attcagacta ttcagcaaaa aagacaagga cagacaatcc tatgctcttg
    40981 ataaagacaa aggaggagta aaatgtcgat ttttagaaaa ttaggttggt tttttcgtca
    41041 ggaaaagaaa cattatttaa ttgggatttt ctccctattc gcagtggcct tggtacagtt
    41101 agttcctcca aaagtgattg ggatcatcat tgatgagatc gccgataaga atttatcaat
    41161 aaagatcatt tcttggtgga tcttactatt gatccttgca gctttcttac aatatctttt
    41221 tcgttatatc tggcggacga atatttgggg aagtgcagct cggttggaga aagaacttcg
    41281 gcagcagcta ttcaaacatt ttacagaaat ggacaatctc ttttaccaaa aatatcggac
    41341 tggagatttg atggctcatg caacgaatga tttgaatgca atccagaatg tagcaggagc
    41401 tggaatcttg acctttgcag attcagtgat tacgggcgga acaacgatca ttgcgatgat
    41461 tttgtttatt gattggcgct taacactgat tgcgctgatt cctctgcctt tgctagccgt
    41521 tacttcacga gtgctggggt caaaactcca cgatgctttt cgtgattcgc aagaagcttt
    41581 ttctagtatc aatgataaga cacaagaaag tatcactggg atcaaagtca ttaaaacatt
    41641 tggacaagaa aaagaagatc tcgcggattt tgaagaaaag attgaagatg caattattaa
    41701 aaacaaacga gtcaattttt tagactcctt atttgatcca ttcattacct tgattatcgg
    41761 tctgtcttat gttgcaacaa ttattttagg tggaaagtat gtcttggaag gcacgatctc
    41821 gattgggcag atggtctcat ttattagtta tatcggtatg cttgtttggc cgatgtttgc
    41881 gattggccga ttgttcaacg tattggaaag aggaaatgct tcttatgatc gcgttaatga
    41941 acttttgcat gaaaagacgc atatcgtgga acaactgggt gcagtgcaga ccccggcaaa
    42001 aggcgagctg aatgtagcta tttcctcttt taactatccg aaagaagaat caccagcatt
    42061 agaacaaatc aaatttgcga tccaagaagg agaaacatta ggtattgttg ggaaaactgg
    42121 tgcgggaaaa acgacaatca tcaaattact gatgcgtgaa tacgatgatt atcaagggaa
    42181 aatccaattt ggcggacgtt cgatcaaaga atattcattg aatgctttat taggatcaat
    42241 cggttatgtg cctcaggatc acttcctgtt ttcgatgacc attcgagata atatccgttt
    42301 ttctaatcca aaactttcag aagaagaagt agagcatgcg gcttctttag catttatcaa
    42361 caatgaaatc aaggaattgc caaaaggtta tgatacctta gttggagaac gtggagtctc
    42421 tttatctggc ggacagaaac aaaggatttc gattgctcga gcattgatca aaaatcctga
    42481 attgctgatc ttagatgatg cactttctgc agtagatgcc aaaacagaag aagctatctt
    42541 gcataattta aaagaaataa gaaaagagaa aacaaccatc attgcagctc accgtttaag
    42601 cagcgtgatg catgcaaaag aaatcattgt tgtcgatcaa gggaaaatca ttgaacgagg
    42661 aacccaccac gagctgcttg cattaggtgg ctggtatgaa cgaatgtggg aaaaacagca
    42721 attagaagcg aagatagaag gaggcgaggc ctgatggatg aaaattatca atcggaatgg
    42781 acaaaatcga ttccacttaa agaacagaag aagatcatca aacgactgat gaagttcgcc
    42841 aaacctttta gaagaacgtt catcgttgct atcttatttg cgtttgccct gtctgtgatc
    42901 aatgtcctat tgccaaggat catccaaact tttatggatg atcatctggc taaacagtca
    42961 gctacgacac aagttatttt gttttttgct ggtgtgtact tgtttggagt catagtcaaa
    43021 agtatcattt ggtttttcca atggtatttg tattccatgg cttcattgaa gacctatcag
    43081 tatgttcggg taagattatt tgaaaagctg catacactag gtatgcgtta ttttgaccag
    43141 acaccggcag gatcgaccgt ttctcgagtg acgaatgaca cagaaacgct atttgaattt
    43201 tggtatgtct ttttgatggt attgactggg atctttgcag tcgtttcttc tttcgttgcc
    43261 atgtttcaaa tcaatggaga aattgccttg tggactttgg tgttcttgcc gattttagga
    43321 atcgtcatct ggtattacca gaaattcagt tctcgtattt accgaaatat gcgggaaaaa
    43381 ttaagtcagc tgaacacgaa attgaatgaa tcgatttccg gcatgcgtat tatccagcag
    43441 tttcgccaag aagctcgttt agctaaagaa tttgaacaag tgaacaacga gtatttagac
    43501 acgcgctatg cgatgatcaa aacgaattcg attctgttaa gtccgatcat caattttctc
    43561 tatgcattgg cgattgcgtt ggcgttgact ttgtttggga tcgatgcgtt gcattctccc
    43621 gtagaagttg gactgatcta tgcatttacg acctatgtcc agggattttt taatccaatg
    43681 tctcaaatga tggacttttt gagcaccttt acagatggga tggttgccgg tagtcgtatt
    43741 ttgaaaatca tggatacaga agaatatgca ccgcaacaaa atcctggagc agatcaagcg
    43801 attacttcag gtaagatcga gtttcgcaat gtaagctttt cttatgatgg agagcacaat
    43861 gtattgaaca atatctcttt tgtcgctaat ccgggtgaga ccgttgcatt agtggggcac
    43921 accggaagcg gaaaaagctc gatcatcaac gtattgatgc ggttttacga attttatgaa
    43981 ggccaaatct tgattgatga tcatgacatt cgagaatatc caatggaaga gttacgtaag
    44041 aaaatgggat tggtcttgca agatgccttc ttgttttatg gagatattgc aggaaatatc
    44101 cggatgatga atccagctat cacagacgaa cagattcgcc atgcagctga gtttgttcaa
    44161 gcaaacaagt tcatcgatga attaccaaaa ggatatcact caaaagtgat cgaacacggt
    44221 gccagctatt ctagtggaca acgacagctg atcacttttg cgcgaacaat cgtgacagat
    44281 cctaaaatac tagtattgga tgaagcaaca gcaaatatcg atactgaaac cgaaggattg
    44341 atccaagaag gattggcaaa aatgcgacaa ggacggacaa cgattgcgat tgcccatcgg
    44401 ctgtccacga ttcgggatgc agaattgatt ttagttttag acaaaggtca tatagtagag
    44461 cgtgggaatc atgaaacact tctagcgcaa aatggtttgt atgcagatat gtacaaactg
    44521 caaagcagtg gtgtgtaaaa agaggttgtg acagtagcat agtagcattt tgtcctaagc
    44581 gctagagtga aaataaaaca gagcttccca gattcttcat ctgtgagcgt tagtggcgta
    44641 gtcacccgat ttaaaaaaga cgacgacagc tagtcaaaag tgcctaatag ccactaagaa
    44701 agcgttgaca aaagcgtttg ctttcgataa tataaagaaa acgatacaac ttggatagtg
    44761 attgcctgat gcaagctaaa cctgatgagt ctcccatggt tccatggacg gctttttggg
    44821 tttttctttt tgttttgaat acagccctat atatcatgga aaggagcaaa acatgctgat
    44881 tgagaaagta ctgaataaca acgtcgtaat aaccagggat gaaagaaatc aggaaatgat
    44941 cgtgatggga aaaggtttgg cattcaaaaa gaaagtgggt gacccgattt ttgactcatg
    45001 catcgacaaa aaatttcagt tgacaaatga cgggctttca caacagttcc aagaattggc
    45061 acaagcgatt cctcttgagt acttagaaat ttcttgtgaa atcattgaat atacaaaaga
    45121 ggctctacaa gtccagttga atgattctgt gtatattgca ttgacggatc atttgtatac
    45181 ggctatcgaa cgtcacaaaa tgggaatatt tgtaccaaat gtattgctgt atgatatcaa
    45241 acaactattt cctaaagagt atgccatcgg gaaaaaaaca gtgaagaaaa tctcggataa
    45301 ataccacatc aagttatcag aagatgaagc cggatttatc gcattgcatt tagtcaatgc
    45361 acaaggcatt caaagccaac aaaacaatac ctacctgttt actataacaa tacaggatat
    45421 tctaaatatc gtccgctatt actataagat tacatttgat gaagattcag tccatttttt
    45481 tcgattcgtc acacacttac gtttttttct gacacgcgtc ctagatggag aaaaatcaga
    45541 agtgggagaa gtttctgagg aattgtttca ggttattcaa aaaaaatata aggatacgtt
    45601 tggttgcgtt gagaaaatag cagacttttt agccgagacg ttcaactacc agatgtccac
    45661 ggacgaaaaa atgtatttaa cgatccatac tgcgcgttta gtgcatgaga atcgttgtaa
    45721 ataaataaaa aaaggaggat aaattaacaa gaacgtatga aataagggta gtgacattca
    45781 gttggctaaa ccttccacta atatattgga gggaatagaa atgggaaaat accaagaatt
    45841 agcgagaaaa atcgtagaaa atgtcggtgg gaaggaaaat atcaatggtt tgacacattg
    45901 tatcacaagg cttcgtttca aattaaaaaa cgaagaaaaa gcaaatgatg agatcttgaa
    45961 aaatatggac ggtattgtaa cggtaatgcg agcgggaggg cagtatcaag ttgtcatcgg
    46021 gaatcatgtg ccagtcgtgt ttgaagaggt tataaaagca ggaaacctta cttttgatga
    46081 ggctgtttca acagaaaaaa tgagaccgtt tgatatgctt attgatggaa tatcgggctg
    46141 tttccaacca ttccttgcta tcttagctgc tggcgggatg attcgtggac tgacagcatt
    46201 ccttgtcttc ttaggcgcgt ttgaccgcgg ttctggcaca tttgttatgt ttgataatat
    46261 cggtgattct gtattccaat ttatgcctgt tattatcggt ttaacagctg cccgtaaatt
    46321 taaagtaaat gaattcgtag ggatgttgct tggcgcagca ttaatgaatc ctagcttgtc
    46381 gctggaagct ttgagtggtg cagcagaagc accactaacg accatatttt ctggcactat
    46441 ttttgaagca cctatttatc aaacagtatt cggcattccc tggattgcgc gtaactacgc
    46501 ttcttctgtc attcccatta tctttattgt gttgttagct agccaaattc agaaaccaat
    46561 taaaaagtta gtacctgaga tgatcgcgaa tttcttcgtt ccattcttca ccgttcttat
    46621 tacaatgcct ttaggttttt tactcgttgg accagtattc acattcgcaa ctgatatttt
    46681 aatggctgga tttgaaacac ttttagcact ttcgccagtt atttatggag caatcgtagg
    46741 cttcttctgg caaattctgg tgatgtttgg tctccattgg gcaatcgtac caatgggatt
    46801 gatgcaattt tctgtgaatg gttggcaaaa tattatgaca cctgttgcag tagtgtcttt
    46861 tggacaaact gcagcactta ccgcactcta ctttaagctt agaaatccaa aagacaaagc
    46921 aattgcaata ccagccattg tttctggtat tgtggggatt acagagccag ctatttatgg
    46981 gtttacatta ccacgtaaaa aaatatttat ttttacttgt gtcggtggtg cgttaggggg
    47041 cgcgtatagt gggttaatga atttaacttc ttggaatcaa ggtggactag gcatctttac
    47101 aattcccaac tatattagac cagatggtga tttaacagat gtgattaatg tattgattgg
    47161 gatcgccatt gcgatggtgg tatcctttgc gctgactttt ttcttttgga aggaggaagc
    47221 aggtgaaaca gatgacagac agaaaagatc aggtaaagaa attgtaaaaa caccgataca
    47281 aggacaaatc gctccactga aagcagcaaa ggatgcagct tttgcacaag gaacactagg
    47341 tcgaggaatc ttgatctacc ctgaaaaagg agaagtacgt gccccttttg acggaacagt
    47401 catgaccctg ttccccacga aacatgcaat cggaatggtt tctgaaacag gtctggaatt
    47461 gctgattcat gtcggattgg atactgttca gttggaaggg aaatattttg aaagcttggt
    47521 gcagcaagga gaaagagtga aacaaggcga tctgttgctc cgcttcgaca aaaagaaaat
    47581 cgaaaaggaa ggatacagtt tagaaacacc tgtaatcgtc accaattaca tggattattt
    47641 ggatattttg gaaaatcaag gggagacggt cgggccagac gatgcactta tcaccgcact
    47701 gacctgaacg gttaggtgaa agaatggaat agacttaact attcttttta gtccacttct
    47761 ttattttcaa aattgaaagg ctctttcatg atggctgagc tgatagggcg tttatcaaat
    47821 ttttgtccta cagaaattag atggactaaa ttctgttgat aattttctgc aaaaacacaa
    47881 ctatataaca cgtctagaag ataacaaatc gatgtattga tcgtaaaatt accaattttt
    47941 gaatacagtc gttcgcgtgt cgtaagtcgc aaagcggctg tactcattga ggatagcgtt
    48001 gaatcaccga tactggtcaa cgctaaaatt ggaacatgat tttgtttcaa tatttctgcg
    48061 gtacgaagga tcacattgtt ttcacccgta taagaaatca gaatcccgca catatcactc
    48121 gtgcaattgt acgcttcata gaacgattca ccaaaagtgg tgacaatcgt gacatccatt
    48181 ttgatgcgct tcattttcag catgaaatct tgagaaatca aagtattcga gttactggcg
    48241 aaaatcacga ttttttttgc ttgaagcatt agttgtttgg ctttttgcag ttggtcatga
    48301 tgaatgagag aaagtgtatc ttgaatcgtc atctgttcta aagaagccat tttatttgcg
    48361 atcgtcataa taccgtctgt ggtggaaaaa ggaagattcg catctacgtc ttgaaaattc
    48421 ccttcaagat aggctgtttc agctaaaaaa gcattacgaa aactgaccca gccatcaaaa
    48481 cccatttttt ttgctacacg gatgagtgtg gacgggtgga cataacatcg ctcagcaatc
    48541 tcttggatgg tcatttcgcc aagtgctgga gaatcgctta agagaaactg aatcacttca
    48601 ttttctgaag gagaaaagga gatttctttg attttttcac gtaataacat gtagatacct
    48661 tcttctagtt tttttacgta aattataact tttttgaaaa tgatttgtaa acggatttat
    48721 gttatatagc ttaataaagt cattttttga caagatattt acaaaaatcc agtagaaaac
    48781 actaactttt gtaaatgtta ctggaaagaa acgaatttct attgtttgtt ttgtttacaa
    48841 aaaagataat aaaagtgtaa aaagcaaagg agtgagaaca tggcaaaagg aatcaaaatc
    48901 gctacgattg gtggaggcag cagttacacc cccgagttga tggaaggatt tatcaaaaga
    48961 tatgaggaat taccgatacg agaaatctgg ctcgtagatg tagaagaagg aaaagaaaag
    49021 cttgagatcg tcagtcagat gtctcagcgt atgtgggatg cttctccgta tgaagtcaag
    49081 atctatgcaa ctttggatcg ggaagaagca ttgaaagatg cggattttgt tacgactcag
    49141 tttcgtgtcg gtcaattaga tgcacgaatc aaagacgagc ggatccctag ttattatggg
    49201 atgattgggc aagaaacaaa tggtgctggc gggatattca aagcttttcg taccgttcca
    49261 gtggttttgg caatcgttga agacatgaag cgtttatgtc caaatgcttg gctgatcaat
    49321 ttcgcgaacc caagcgggat ggtgacagaa gcagttatca gatatggcaa atgggataaa
    49381 gtgatcggat tatgcaatgt gccagtaatg gcgacgatga cagaaccagc aatgcttggc
    49441 aagaaaaaag aagagctgat ctacaagttt gccggtctaa atcacttcca ttggcacaaa
    49501 gtagcagata ataaaggaaa tgaccgcaca gcagatttga ttgatcgttt atacgatgaa
    49561 gacaatggat tgccgaaaaa tatctttgac atcccgtttt tcaaagaaca attaaaacaa
    49621 atgaacatga tcccttgtgg ctaccaccgc tattactacc gtgaagaaga aatgttggaa
    49681 catgctttag aagaatatca aacgattggt acaagagcgc aacaagtgaa agaaacagaa
    49741 gcagagctgt ttgaattata caaagatcca gaattggatc ataaacctga acaattgcaa
    49801 aaacgaggtg gtgcttatta ttcagatgct gcctgtgaaa cgattgcttc gatctatgga
    49861 aataaagaaa cacagatcgt ggtgtctaca aagaatgatg gagctgttcc tgatctgccg
    49921 gcagattgtg tagtagaagt cactgcctat gttggcgcac aaggcgcacg aaatgtagcg
    49981 tttggttccc ttcctgctgc tgaaagaggc tggttgcaag taatgaagaa tatggaattg
    50041 ctgacgatcg aagcagccgt gactggtgat tatggaacag ctttacaagc gtttacgatc
    50101 aatccgctta tacgatcagg aaagacagca aaacggatca tggatgagtt gtttattgca
    50161 catcagcagt atttaccgca attcaaggaa acaatcagcc ggttaaaagc agaaggaatc
    50221 gaagtccaag atgaactggc aagagaattg tcaagcaata cacatgtaat ggaggaagta
    50281 tgatgggatt cagaaaagat tttttatggg gcggtgctac agcagccaat caatgcgagg
    50341 gtggttatga tgaaggcgga cgtgggttag cgaacgtaga tgttgtgcca atcggagaag
    50401 accgttttcc tgtcattact ggaaaaaaga aaatgtttga atttgataaa gatcattttt
    50461 atccagcaaa aattgcgatc gatatgtatc atcattataa ggaagatatt gctttgtttg
    50521 cagaaatggg gttcaagact tatcggatgt ccattgcttg gagtcgtatt tttccaatgg
    50581 gcgatgaaaa tgagccaaat gaatctggtt tgaaatttta tgaggatgtc tttaaagaat
    50641 gtcaaaagta cgggatcgag ccgcttgtga cgattacaca ttttgattgt ccgatgcatt
    50701 tgatcaaaga atatggtgcg tggcgttcta gagaactcgt tggtttttat gaaaatctgt
    50761 gccgagtcat cttcaatcgc tataaaggac ttgtcaaata ttggttgaca ttcaatgaga
    50821 tcaatatgat ccttcatgca ccattcatgg gcgcagggct gtattttgaa gaaggagaaa
    50881 atgaagaaca aatcaaatat caagcggcac atcatgagtt ggttgcaagt gccatcgcaa
    50941 cgaagattgc tcatgaagta gatccggaga acaaagtagg ctgtatgctg gcagcaggag
    51001 catattatcc atacacttgc aatcctgaag atgtttgggc ttctagaaaa tcagatcgag
    51061 aaaattattt cttcattgat gtccagtcac gaggagaata tcccgcttat gcattgaaag
    51121 aaatggaacg aaatggcatt cagatcaaaa tggaacctgg ggatgccgca ttgctgaaag
    51181 aacatactgt tgattttatc tccttttctt attattcttc tcgagtttcg actactgaca
    51241 acgaactctt ggaacagact gcaggaaata tctttgcttc tgtcaaaaat ccatatctgg
    51301 aagcaagtga atggggttgg caaattgatc cattaggctt gagaataacg atgaatgata
    51361 tttatgatcg ttatcaaaaa ccactattta ttgtagaaaa cggcttaggt gcagtcgata
    51421 cgcccgatga gaatggttat gtggaagatg actatcgaat cgactatctg gcagcacata
    51481 tccaggcaat gaaagataca gtagaattag atggagtcga tctattaggc tatacgactt
    51541 ggggatgtat tgatttagta tcagctggta caggtgagat gaagaaaagg tacggattca
    51601 tttatgtcga tcgtgacaac gaaggaaatg gcacattagc ccgttcgaag aagaaatcat
    51661 tcaactggta taaaaaagta attgattcaa atggagaaaa tctttcgaat gattgatcaa
    51721 tgatttgtaa aagtctattg tttttcagcg gatttttcga gtataataaa gctattcgag
    51781 aaacgcaagc agaacaatag actttttttc gcattggctg taaagtatgc ttacttgatt
    51841 aagtaaagga gaatctacat atgtatgatg taatcgttat tggcgcaggt cctgctggga
    51901 tgactgctgc tttatatgct tcacgttcaa acttatccgt attgatgatc gaacaaggcg
    51961 cccctggcgg ccaaatgaac aatacggcag aaatcgaaaa ctatcctggt ttcgattcaa
    52021 tcatgggtcc tgatctagct tataagatgt atgaaggtgt ttcaagattt gggacagaaa
    52081 atgcttatgg aatcgtgcaa gatatcaagg atcacggcag ccacaaagaa gttatttgtg
    52141 aagataaaac ttacgaagga aaaacagtca ttatcgcaac aggctgtatc caccgtaaat
    52201 taggcgtacc tggcgaggaa gaatttgctg gacgtggcgt ttcttactgt gcagtttgtg
    52261 atggtgcgtt tttccgtaac aaacgtctag tagtgatcgg aggaggagac tcagcagtag
    52321 aagaagccat ctatttgaca caatttgctt ctgaagtagt catcgtccac cgtagagatg
    52381 aattacgtgc acaaaagatc atccaagacc gtgcatttgc gaatgaaaaa atttccttta
    52441 tgtgggatac agtcgttgac gaaatcgttg ggaatgaaat ggtagtgaca ggtgtgaaag
    52501 cacgtaatgt caaaaccggc aatgagtttg atttggcagc agacggagta ttcatctatg
    52561 ttggactgga tccattaact gcaccattta gagaagcagg gttgacgaac gaagctggct
    52621 ggattccaac agacaacgaa atgaagacga atataccagg cgtttttgcc atcggagatg
    52681 ttcgtgaaaa agatttgcga caaatcacta ctgctgttgg tgatggtggg atcgctggac
    52741 aacaagtgta taaatacttg gaatctttag aagaaacaac tgcataagtt taaaaagagt
    52801 ggaagaacgg tttcaattgt tcttttactc ttttttttcg cgtgaaatct actgaatccg
    52861 atttatgcga atacttgcaa ccgtgcttga taaaagaatc ctttcttgat ttaagcgttt
    52921 tttagacctc gtactttcat atctaataag tgaatgctat acttattaag aaccatattt
    52981 cagaaagagg tgttattttc atgtcttggg aacaagtgta tgaacaatgg gtacatgaag
    53041 aaaatttaga agaaaactta aaaaaacaat taattgatct gggtgaagat cctgaaaaac
    53101 ttgaagatgc tttttatgca ccgttggaat tcggtactgc aggcatgcgg ggaatcttag
    53161 gacctgggat caatcgaatg aacatctata cggtaagaca agcaacagaa ggacttgcac
    53221 gtttcatgga tacacaaggt gaagaaacaa aacgccgagg tgtcgctatc gcttatgact
    53281 cacgccatca atcaccagaa tttgccatgg aagcagccaa aacattagca catcacaata
    53341 tcccttctta tgtattcgaa agtttacgtc caacaccaga gctttctttt gcagtccgtc
    53401 atttgaagac gtttgcggga atcatggtaa ctgcatccca caatccagca gcttacaacg
    53461 gatataaagt ctacggagaa gatgggggac aaatgcctcc tgcagacgca gatgcattga
    53521 cgaaatatgt ccgtgaagtc agcaatccgt taaaagttga agtattatct gacgacgaag
    53581 ctgaacatag cggattgatc acgatcatcg gagaagaagt ggatgccgct tatctagaag
    53641 aaatcaaagc agtaacgatt gatcatgaat tggtggaaac aatgggtaaa gatttgaaac
    53701 ttgtgtacac accacttcac ggaacaggaa aaatgttggg cgaacgtgcg ttgaaacaag
    53761 ctggattcga acaattcgta ctagttccag aacaagctgt cgcagacccg gatttcacta
    53821 cagtcaaatc accgaatcca gaagaacact ctgctttcga gtatgcgatg cgacttggcg
    53881 agaaagaagg cgctgactta ctgattgcaa cagatcctga tgcagaccgt ttaggcgcag
    53941 ccgttcgttt gccaaatggc tcctatgaag tattgacagg gaaccaaatc ggagccctgt
    54001 ttgctcaata tattttggaa gcacaccaat cgcgaggcac tttacctgaa aatgcagcgg
    54061 tattgaaatc aatcgtttct agcgaattgc ctacagcaat tgcggaacac tatggggcta
    54121 ccatgttcaa tgtattgaca ggattcaaat tcatcgctga aaaaattcaa caatttgaag
    54181 aagatcattc ccatactttt atgttcggat tcgaagaaag ttacggttat ttagttaaac
    54241 ctttcgttcg cgacaaagac gcgattcaag cattgctctt attagcagaa gtagcagctt
    54301 actataaaaa acaaggcaaa acgttgtatg acggcttgca aacgattttt gaaacatatg
    54361 gctactacgc agaaaaaacg atttctgtga caatgagcgg acaagaaggt tctgcaaaaa
    54421 tctctgcttt gatggaaaaa ttccgtgaag aagcaccgac agaatttgcc ggagcaaaag
    54481 tcatccaaac agaagacttc aaagagttga cgagaacaga ccgtgaagga aacgtagaaa
    54541 aactaacgac accgccatct gacgtattga agtatacgct ggaagaaaat ggctggatcg
    54601 ctatccgtcc ttcaggaact gagccgaaaa tcaaatttta tatcggtgta aaaggaaact
    54661 cttcagaaga cgcagctgaa aaagtagccg cttatgaagc gattatcaaa aaaatgacag
    54721 aagaataaaa aaacttgaga ctgatggaaa aagtggtaaa ctttagaagt cagtcttttt
    54781 tcaagttgga ggtgaatgga ttgccagaca cgcaaaaaac aaaagtagag aaagtcagcc
    54841 aactattcaa aatgttgagt gaccccactc gtctcaaaat tctgctgtat ctgaaagacg
    54901 gggaacaaaa tgtgacagca atcacccaag cagtagaaat ggaacaatct gctgtatcgc
    54961 atcaactgcg tttattaaga gaaaaccatg tcgtcaagtc ccatcgtgag ggaaaagcga
    55021 ttttgtatag tcttgatgat tctcacgtac tggatatttt gaaccagacg ctcaaacatg
    55081 tggaacatca ataacgatag gtgcaaatag agacaaactg gactgtatca acgaatcttc
    55141 ttcgctgaca cagtccagtt tttttaagaa aaagacaaga aaaactgtta gtctgtttct
    55201 tcggtggttt cagttccata ataactatga tcttctaggt ctaaagcatt caaaataagt
    55261 gaagctgttt cttcgataga taaagaagcg acattgatta ctacgcagcc caatttttca
    55321 taaagatcat tcgcaaaatc gagttcagct cgtatttttt cgatatcgga ataagcagta
    55381 tcaggattta atccgtaaga gcgcatccgt tctttacgga tattgtttaa aatatctggg
    55441 ttattcgtca gtcccacgat ttttttagga tcgatctccc aaagctgttt gggaatatgt
    55501 gcttgtggga tcaaaggtag attggcaacc tttaaatttt tgttagctaa aaacaagctc
    55561 aaaggcgttt tggaagttct ggatacgcct agcaaaacga catctgcttc tagaaaccca
    55621 cgaggatctt tcccgtcatc gtattttaca gcaaattcca ttgctttgat ccgtttgaaa
    55681 tagttctcat tcaataaatg aagggcgcca ggttgccgaa tgggcttgat accagttaag
    55741 cgctcgattt ctgatatcgg cggagtcatc acatcaaaac taaataattg atgttcttca
    55801 caaaaagaac gcgtcatctc gatcaattct tgattgatca atgtgtgcag gacaagggct
    55861 ttttctttta gtgcatcttg cagtgcctgc atcaatgttt tctcatctgt cacaaatgtt
    55921 ctgcggaata aattgaactc aacggtagga tattgggcca tagtggcttg cgctaatttt
    55981 gtcgcagttt ctccagcaga gtcagagatg acaaaaaaag taacgatttc tgtagaatcg
    56041 gaagacatgg ataaaacctc acttgcattt tttttatgtt tattatacca tactctagaa
    56101 tgtgtacttc gtaatcattt tgatttggag gaataagtca tgaaaaaaca gacattgatt
    56161 gaacaatcac tagaaacatt gaaaaataac ggttttaaat atactaaaaa aagagaagca
    56221 ttgttaactt atttagtgaa gcgcaatcgc tacgtatccg ctaaagaagt ttttgactat
    56281 atgaatcagg aattcaaagg agtaagttac gatacgattt atcgaaatct ccatgatttt
    56341 gcagaaatcg atttactgga agaaaccgat ttaaatggag agatgaagtt tcgttttcat
    56401 tgctgtcaag gggaaatcgg ccatcaccat cactttatct gtacggtgtg cggaacaacg
    56461 aaagaaatcc atatgtgtcc aatgaattat ttcgaagaac agctcggtgg atgtacgatc
    56521 gaagggcata gattcgagat tttcggaaga tgtgaaaatt gtcaataagg atttaaataa
    56581 aataacattt gataatttta aaaaaaagca agatttcctt gacggtcaaa acagcattag
    56641 atataatatt tacaggacag tcttgttttt tgagatatat ttcaataaca attacggtta
    56701 ctttttaagc tcggagggag ggaattaaca tgtcaaaaac agtcgttcgc aaaaacgaat
    56761 cacttgacga tgctcttcgt cgcttcaaac gttccgtgtc aaaagcgggt actttacaag
    56821 aatctcgtaa acgtgaattc tacgaaaaac caagtgtaag acgcaagaaa aaatctgaag
    56881 cagctagaaa acgcaaaaaa ttctagttct gtgatgaatt ggagttggta gtgtgtcact
    56941 actaagtaga ttaaacgatg acatgaaaac agcgatgaaa gcgaaagata aggaatcttt
    57001 acaagtcatc cgaatgatca agtcatctat acaaaacgag cagattaaag aaggtcacga
    57061 cttgacagaa gaagaagaat taacggtgtt gtctcgcgaa atgaaacagc gacgtgattc
    57121 tttgcatgag tttgaagaag caggacgtga cgatttagct gaaaaagtaa aaagtgaaat
    57181 tgtgatagtc gaaaaataca tgccggaaca actttccgac gaagaaattc gacaacttgt
    57241 tcaagaagca atcacacaaa ctggtgcttc atcaatgaaa gaatttggca aagtcatggg
    57301 tgcgatcatg cctaaagtca aaggaaaagc agatggaaac caagtaaatg ccattgtgaa
    57361 agaactatta caatcttaat tttatcattt aaactgcgga caatcattgt ccgcagtttt
    57421 tttgtgcgcg taaaaaagag aacaaaaaaa tagctagacg aaagaatagg attctttcat
    57481 ctagctggtt ttttcctgtt taatatttct tatgttaaat tgtgtttgat ttggttattc
    57541 ttctggtagt tcatttgctt cttgtttata ccctgatagg cgataggcgt aataaaacag
    57601 taggctgcca gcgtaaccaa gtaacgttgg gaccatccag ccaagtccta atgcagcaaa
    57661 aggaacgtga tgatgataaa attcgagcat gcttgctaca actctttgtt gactcactaa
    57721 agcaggcgaa tttgccagca tatccaatac ggcaggaaca gcagcaaaca acatcgttac
    57781 ttgatagaca aatggtgtct tttggaaaaa ttttgctgtt aaggaaagca agatcaacgc
    57841 aagcgacaat ggatacaaaa gcatcaatac tggtaaagac cattggatga tattgtctaa
    57901 accagcattt gcaacgacaa aagagacgaa agtcgttaac cgcagccatg tcatataact
    57961 gactttaggg aacagtttat ggaaatcttg cgcaaaagaa acaacgagtc ccatagcggt
    58021 agtgaagaca cctaatgtga ccatcactcc taagaagcta gcgccgaagt tacctaaata
    58081 aaaattagtg atctgagaaa gtgcagttcc gccattttct gataaagaca tcttattcaa
    58141 actcatcgca ccgagaagaa caagaccaaa atagatcatg acttctagcg caacgctcag
    58201 gctaccagct tttgctgtta attctgtgac ttttttatct ttgaaaccaa ggtcacggac
    58261 tgcatggaca aaagtgacac ttaaggcaag caaggctact gcatcaaccg tgttataacc
    58321 ttctaggaca ccatttgtta aagcgtttgt ttggtaagta tctgcagcag attgatttag
    58381 atttcccatt gggtggaata aagaaacgac aaaaatgatt gctaacaatg caagaaacaa
    58441 ggagtttaga tactttccga cgtatttgac caaccgtgtg ggatggacag taaggaaata
    58501 agcaagtaaa aagaaaagag cagagaataa aaacatacct aatgaattat atttttcagg
    58561 caaaaatggt ttagcagcca tttcaaaagc agtagcagct gtccgtggtg taccgaaaaa
    58621 tggtccgatc gtcaaatgaa caaggattaa gaacaaggca gcataccatt ttccgacagg
    58681 tttagctaag tcatacagac catcactttt tgtcactact acagctaaga tagctagtaa
    58741 tgggaaaaga gcgcccgaga tggcaaatcc tacgccagca ctgacccagt ttgcgccagc
    58801 catttgacct aagtggacag gaaagattag attacctgac ccgaagaaca tcccgaagat
    58861 aagtgaactg accatcaata gttgtttcca tgaataagtt tttgtttctt ccataaattt
    58921 ttcctccaat aattatcatt ttgattgacg atataaacgt tgcagcaatt agtaatgcca
    58981 ctaatatcag attccatgaa aaccaccacc ttaaaatata aaaaaagccc ttttccactt
    59041 gggaaaaggg ctctagattc tagaacggta ccaccttttt tcgcttaaaa aagcttcttc
    59101 attcacggac gctttttgat aaagttatcc gctagcctga taatggaggc agccacaatg
    59161 atctacgttt tcgacattga ctcggaaatg atgttcgtct attcgattta ttgtcatttc
    59221 tcacttacat gactcactgg gaataaatcg ttgaatagcc tactgttttc ctcgacgctt
    59281 tgctgtatgt ttatttgttg acgtaataat acacctgttt tttttgaaat gcaagaatat
    59341 tttaactatt ttgttttctt tttttgaaac aagaaaaata cctagagaat acctgctgaa
    59401 tgaaacggaa tattttgttc tccactttca tataacttac attctcagct tctttttatc
    59461 aaaacgagac ctttagcgaa tggaaacggc tattttttaa cgtttcttgg tacgtatggt
    59521 taaacgactc ttttacaacc ttttcttatt tgctatatag tttcttctga gtatggtaaa
    59581 ataggaaaga atgaatcgaa aaaaagggga tgtctatttt tgacggaaga gtctagttct
    59641 ttagagatta gattgacagc tgctgatgat gtcagcatgc ttttaggtgc acacgacaag
    59701 catataaaac tgatcgagga aacaacggaa acaaccatcc atacacgtgg agaaatcatt
    59761 cagatcattg gtgagcaatc ccagatttcc ttggctgcct cagtcattcg gacattacag
    59821 gaattgatca aacgaggaat ccacgtaagt acgccagatg ttgtgacggc attgaagatg
    59881 gggcaaaaag gtacattaga ttattttgtt gagatgtatg aggaagagat cgtcaaagac
    59941 cgtaatggaa aacctatacg ggtaaaaaac agcggtcaaa agaaatacgt tgaatcggta
    60001 gccaaacatg acatcgtatt tggtgtaggg ccagccggaa cagggaaaac gtttcttgcc
    60061 gtagtattag caattgctgc cctaaaaaaa ggcgaagtgc aaaaaatcat tttgacgcgg
    60121 cccgctgtgg aggcagggga aaacctaggc tttctacccg gggatctgaa agaaaaagtg
    60181 gatccttatc tgcggcctgt ttatgatgct ttataccaaa tttttggatt ggatcatacg
    60241 aaccgattga tggaacgagg cgtgatcgaa atcgcacctt tagcttacat gcgggggcgg
    60301 acattggatg atgcattcgt tatcttggac gaagcacaaa acacgacagt cgctcagatg
    60361 aaaatgttct tgacccgtct cggttatcaa tcaaaaatga ttgtcaatgg ggatacaagt
    60421 cagatagact tgcctcgtgg gacgacaagt ggtcttgttc atgcagaacg aacattaaag
    60481 caaatcaaaa aaatcgattt tgttaatttt gaagcaagtg acgttgttcg gcaccctgta
    60541 gttgccgaaa tcatcaaagc ttatgaaaag gcggatcttc atcaagaata agctttgctt
    60601 aggaggataa gcacatgttg aataaaacac tgcagaaact acaaaaaaaa ctcggaaagg
    60661 cagcattgcc tttccttctg ttcttgtttt ttatcattgt ctgcggcgtt acatttagta
    60721 gtgtcaaaca aaaggccaat gatttcagag aaggccaagt agcagaagag agtatccgtg
    60781 cgaataaaac gattgagaac acggaagaaa ccgaacaaag aagaaaactg gcagcagaag
    60841 cagtgactcc agaatatact tatcaaaaag atctggcaga tgaccagaac aatcggatca
    60901 agcaattatt cgaattgatc gataagacga atgataccat caacaaatct tataatgaca
    60961 aggtagataa ggcgaaggaa gatgaaaata tcccgaaacc tactacagaa gagcggattg
    61021 ctacactgaa gaaaaatttt gaaaacgtga atgcagaaaa cgttgcgttt taccaaaaac
    61081 tgccgaacaa tttttattcg acgatatttg aaatgactga aacggaaatt cacacggtta
    61141 gggatgaaag tttgaagctc attgacgaac agatgagtaa acaagttcgt gaaagcgaat
    61201 tggaagcttt taaacaagaa gcagaagatc agattcaata tttgaatgtc acaacagagc
    61261 agcaacaaat gattcgctac ttagtggatc aaggcattgt agttaatgat gtattgaatg
    61321 agaaaaagac agaagaatta aaacaatctg cacgagaagc tgtacaacca gtgatgatct
    61381 tccaaggaga aattattgtt cgcgaaggaa atcaaattga tgcttcagcg atgaaaaaat
    61441 tggaattact aggattgacg aatcaaacga cttctatttt cccactcatt gccatggttt
    61501 tagctgtttt gcttcaaatc ggtgtactga tttataattc acttcaattc caaaatgcag
    61561 gagaaagaac aaaatacgta cttttctacg tgacggcaat gtcagtaagc gtcgtactaa
    61621 tgaaattttt ccaactcttc caaacagaac aagctgctta tattccgctg ttttaccccg
    61681 cggcttttgc tccacttgtt ttaagctttt tcttgaatcg aagagcaggg atcatggctg
    61741 gtatttttca agcagtatcc gcacttttca ttttttatga ttcgattggc acgaactttc
    61801 tgacagttat tttgatgtcc tatgtatttt ctggtctcat ggcaacgatc gtcagacgca
    61861 aacggatctc agaacaaggg ttatccgctg ctatgtgggt cattattttt cctgtgatga
    61921 tggatgtcat tttgattatc taccaaggaa tgagctttgg ggatgcaacg acttggttaa
    61981 cgctagtatg cggattcaca ggtgcactgt tctcattctt gctgacgata ggtctgcacc
    62041 cctatattga attgatggtc aatgatgata gtgtgatcgt attaaatgaa ctcagcaatc
    62101 ctaatcatcc tttattgaaa cagttgctgg aagaagcacc aggcacgtat caccatagca
    62161 tgatggtagc caatctaagt gctaatgctg tagcagaaat cggtggacgt tctttactaa
    62221 ctcgagtagc ttgctattat catgatatcg gcaagatcaa acacgccagc tttttcgttg
    62281 agaacttgcc ttcaggagcg gaaaatccgc acaatttctt attgccagaa gacagtaaac
    62341 atattatctt tggacatgtg acagatggtg ccaaaatatt ggaagattat aatatgccgc
    62401 aaatggtgat cgatatttgc agacagcatc atggaaccac gctgatgcgt tatttctacg
    62461 ttaaggcaaa ggaacgcaac ccggaagtca cggaagagca attccgttat cctggaccaa
    62521 aacctcaaag ccgtgaagca ggaatcgtca atatagctga cagttgtgaa gcggcagtac
    62581 gagcaatgga ccatccaacg agcgaaaaaa tcgaaagttt tgtccataat cttattgaag
    62641 agcgtatttc cgatggacaa ttggacgaca gtggattgac gttaaaagaa atccgtaaag
    62701 tagaaaaatc attgatcagc ggtttaagca gtaccttcca ttctcggatc aaatatccta
    62761 aaatgaaatc ggaagttgag aagataaaag aagaacaaga agaatagaaa gaagcgatgt
    62821 ataatggata ttactttcat ggatgaaaca aaccaagtct cagaagagaa aatgaaagaa
    62881 atcgacgact tgctgcaatt tgcagcatca tatttagagt tgccagaaga aacagaaatg
    62941 tctgttactt tcatggacaa tgcagcgatc caagtaatca atcgtgatta ccgtggaaaa
    63001 gatgcaccga cagacgtcat cagttttgcc ttggaagaag agggcgaaga cgaaatccca
    63061 gtgatttttg aagatggaga gaatccattg cctcgtgtac tgggagacat catgatctct
    63121 actgaacgtg ctgcagagca agcagaagct tacggccatt cgtatgatcg ggaacttggc
    63181 tttttagcgg ttcatggatt cttgcatatc aacgggtatg accatatgac accagaggat
    63241 gagaaagaaa tgtttggttt acagaaagag atcttagatg cctatggact taaaagataa
    63301 aaacgtagag aaaaacaaac atttcctgat gtccgtggaa tttgccgtcc aaggagtcaa
    63361 aaccgttttt gaggaagaac gcaacatgag gaaacatgtt gcgttcggaa tcctcgcttt
    63421 gtttatgggc gtgattttcc agcttgatcg gatagaatgg ttatggctgc ttttagctgt
    63481 ttttctcgta tggatagtag agatcatcaa tactgttttt gaaaatgtcg tggacatgtt
    63541 tacggatttt cattttcatc cgattgggaa aaaaatcaaa gatatggcag ctggagcagt
    63601 tttagtgacc gcttgttttt ccgtcatcgt tgggttgatt ttatttgtcc ctaaaatata
    63661 tcatttgttg tttaattaaa aaggagaacg tatatgccag aacataaatc agggtttgtc
    63721 gcaatcgtcg gccgcccgaa tgtaggaaaa tccacattat tgaatcgaat cgtgggtcaa
    63781 aaaattgcca tcatgagtga taaggcgcag acaactagaa ataaaatcca aggagtatat
    63841 actacaccag aagctcagtt gatttttatt gacacaccag gaatccataa accaaagcat
    63901 cgtttaggag attttatggt agaagcagct tacagtgcgt tgaaagaagt ggacgcggtc
    63961 ttatttatga tcagcgctga tcaaaaacgc ggtcggggag acgattttat tattgaacgt
    64021 ctaaaaaacg tgcattctcc tgtttatctg attatcaata aaatcgataa aatccatcca
    64081 gacgagttgt tgggaatcat cgaagattat tcaacgcaga tgccgtttgc acaagtgatt
    64141 ccaatttctg ctacagaagg aaacaacgtc gaacgtttga tggatgtatt agtcagtgaa
    64201 atgccagagg gcccacaata ttttccagat gatcaagtga ctgatcatcc tgaatacttc
    64261 atcgtttctg aattgatccg tgagaaagtc ttgtttttaa cacgagacga agtgcctcat
    64321 tctgtcgcag tcgttgttga ttcgatgaag cgaaacgaga atgataaaat ccatatccag
    64381 gctacgatta ttgtcgaaag agatagccaa aaagggatca ttatcggcaa aggcggaaaa
    64441 atgctgaagc aagtaggaac gaaagcgaga aaagatatcg aaaatctttt aggagacaaa
    64501 gtctttctag aactttgggt caaagttcaa aaagactggc gagacaaaaa agtctatctg
    64561 caagacttcg gctaccgaaa agacgaatat tgatattctg agcttcttgt aagttaggta
    64621 tagaaataaa ctggataaaa atccgcgtgt tgagaaaagc tgcggaagat cgcaacataa
    64681 atgatctgga tcggcagaat ctgcttttcc agatttattt tttaggaggg agaatatgag
    64741 ccagcttgca gaatcaaaag gcttgatttt atctagccgt aattacaaag aaaaagataa
    64801 attagtcaaa atctttacgg aatcttctgg caaaatgatg ttttatatca aagggatcca
    64861 tcggcaaaat cagcccttag ctccggcttt gctccctttt actgaagcag tttatatcgg
    64921 gaattttcga agtgaaggtt tgtcattttt aagcagtgtc aaagatgtcc acgcgtttcg
    64981 acacatccaa gaagacatct ttttgagcgg ctatggcaca tatttattga atctagtaga
    65041 tgcggcaatc gaagatcatc agtacgaccc gcatttgttc cagtttacgc aacaagcact
    65101 gcaactgatg gaccaaggag atgatgcaga aatcatcacg aatatttttg aagtccaaat
    65161 actgcaacgc ttcggaattg caccaatctg gacacactgc gtcgtatgtg gggaaacaaa
    65221 aggaaaattt gactattctt ccaaatatgg tggtgtgata tgcgaaagac actggtcgat
    65281 ggatgatcac cgttatcatg cagatcctcg agctgtttat ttcgttcgga tgttttcagc
    65341 tatttcttat gataaaatca gtggaatcca tctgaaagaa gaaaccaaac aagcaatccg
    65401 tgaactcatc gaccagctgt atgatgaata tgtcggtatc catttgaaaa gcaaaaagtt
    65461 cattgatcaa atgaagtctt gggcacacgt attaaagacg tcagagaaag acatgtctga
    65521 aaaagatcta cctaaaaaag aagaatagta aatgaaacat acaacaaaaa atcaaaacca
    65581 tttgacaaac acagggagtt ccgtctatta ttaaccgtag atcaaataga tcgttcactg
    65641 agaggatcta ttatagaaac aagaaaactg caaaatgaat acgtacattg accaagatca
    65701 gtaaaaaaca cgtggtttag cgagttcggg agagtgggag ccggacccaa acgatttttg
    65761 aagaggcact tgcgagtacg ataaatgaag ctgccgaatc tttattcgga agtagggtgg
    65821 aaccgcgaat tattcgtccc tatgtctgta atcagacata gggatttttt gtcgttttct
    65881 ttcctgtatt cttatttgac agcaacgctt taaaattgaa aggggcaaaa cagatgagta
    65941 aaaaattgac cgtccaagat atgattttaa cattacaaaa attttggtca tcaaacggct
    66001 gcatgctgat gcaagcgtat gacacagaaa aaggagcagg tacgatgagc ccatatacct
    66061 ttttacgggc aatcggacca gaaccatgga atgctgctta cgtagagcca tctcgccgac
    66121 cagctgatgg acgttatggc gaaaacccaa accgtttgta tcagcatcat caattccaag
    66181 tcgtgatgaa accttcacct gagaatatcc aagagcttta tttagaaagc ttgcgccttt
    66241 taggaatcga tccactagaa cacgacatcc gttttgttga agacaactgg gagaacccat
    66301 ccatgggctg tgccggtgtg ggttgggaag tatggcttga cggaatggaa atcacacaat
    66361 tcacgtactt ccaacaagtt ggtggactgc cttgtaaacc agtaacttct gagatcactt
    66421 atggattaga acgtcttgct tcttatatcc aagaagttga aagtgtctat gatttagaat
    66481 gggctgatgg cgtgaaatat ggcgagatct tcaaacaacc agaatatgaa cattctaaat
    66541 attcttttga agtaagcgac caagacttgc tactaagcaa ctttgatcgt tttgaaaaag
    66601 aagctaaacg ctgtatcgat gaaaatcttg ttcatccggc gtatgattat atcttgaaat
    66661 gtagccatac attcaactta ttagacgccc gcggtgcagt atccgtaaca gagcgtgccg
    66721 gttatctttc tcgcatccgc aacatggctc gtgctgtagc caaaatcttt gtggcagaac
    66781 gcgaaaaatt aggattccct ttgttgaata aaaacacagc aaatccagaa aacgtgaagg
    66841 aggctgaata agatggcaaa agacttatta cttgaaatcg ggctggaaga aatgcctgct
    66901 catgttgtca caccaagcat gaaacaatta gaagaaaaaa cagcaaaatt tttagatgaa
    66961 aaccaattga cttatgatac gatcgaaaca ttttccacac cgcgccgttt agcaatcaaa
    67021 gtaacaaaca ttccagaacg acaagcggac agtgaagaag aagtcaaagg accagccaaa
    67081 aaaattgctt tggacgcaga tggaaactgg acgaaagcag cggaaggatt tgtccgcgga
    67141 caaggtctga cgacagacgc tatcactttt agagaattaa aaggtgtgga atatgtctat
    67201 gtaacgaaat acactcatgg taaggaatcc aaagaagtat tgacaggttt agcagatgtc
    67261 attaaaagcc tgactttccc tgtaacgatg cattgggcag aatacgattt tgaatatatc
    67321 cgccctattc actggatcgt ggcattgtta gatgacgaag taatcccatt cgaaatcttg
    67381 gatgtcaaaa caggcagagc ctctcgaggt catcgtttct taggcgaaga tgtgacattt
    67441 gcgcatgcaa aagaatatga agaaaaattg aatgaacaat tcgtcattgc gtctccgcaa
    67501 aaaagacaag aaatgatcgt tacacaagcc aatgaattag ccgaaaaaaa tcattgggtt
    67561 attgatctag acgaagattt actagaagaa gtcaacaatt tagtcgaata tccaactgtc
    67621 ttttctggcg acttcgaaga aaaatatctt tctgttcctg aagaagtact agtcacttct
    67681 atgaaagaac atcaacgtta tttcgaggta cggaatgatc aaggtatgct gcttcctcat
    67741 tttatttctg ctcgtaatgg tgatcgggta aaattggaaa atgtggttcg aggcaatcaa
    67801 aaagtattga cggctcgttt agaagatgcg gaattctttt ataatgaaga caagaaagct
    67861 tcaatcgacc aatacgttga aaaactaaaa actgttactt tccatgaaaa aatcggtagt
    67921 atctatgaga aaatgcaacg ggtacaagtc attgcccgta tcattggcga acaagtagga
    67981 ctatctgagg aagaattgat ggatctgcaa cgagctgctg aaatctataa atttgatcta
    68041 gtaaccaaca tggtagggga atttcctgaa ctgcaaggga tcatgggcga aaaatatgct
    68101 ctgctgaaag gcgagaaacc tgctgtagcg acagcgattc gtgagcatta catgcctata
    68161 tccagtgaag gagagctacc tcagtctaca gtaggttctg ttttagcgat tgcagataaa
    68221 ttagatagca tcttttcttt ctttgctgtt gggatgatcc caagcggttc aaacgatcct
    68281 tatgccttac gtcgccaagc atacggtgtc gttcggatca tcgaatcgaa aaaatgggcc
    68341 tttcctttgt ctgttctgca agaaacgatt agcgatgtta tcagtaaaga tacggatcgt
    68401 ttcggcattg gtttatcaga agggcaacaa caagtgatcg actttatcaa aggccgtctg
    68461 cgccaattat tgacaacgaa gaatattcgt catgatgtca tcgatgctgt tttgaatgcg
    68521 gaacaaaaag atctgacaaa agtatttgca gcagcacaat tgttcaaaca acatcttgca
    68581 gatgaagact tcaaaccttc aatggaagct ttgacacgtg tcgtgaactt agcgaaaaaa
    68641 gcgcagcttg aaaaacaatc agaagtagat cctgagctgt tcgaaaacga cgcagagaaa
    68701 gaattacaca aagcagtaga agcaatgaaa gcaagttttg ctgaccaaac gatccacgaa
    68761 aactacgacc agcttgtcgc tcttcgtcca ctgatcgaaa attattttga tcaaacgatg
    68821 gtcatggctg atgaaccagc agtacgtaag aaccgactaa cacaacttag tcaaatagct
    68881 gccatggctt tgtcattagc aagcttggat caaatcatca caaaatagtc ttttgtttga
    68941 aaaacagaga gcatgcacga aacgtgctgt gtctctgttt ttttgttata aatataaata
    69001 gctgttatta attctatttt aataattttt gttattttat tctttgttcg aattttcaaa
    69061 atgctcatga aaagagagga aataaaaatg aaaacgatta atcatgatct tgttactgtt
    69121 ttatttactt ttaaattagg aatttccg
//
LOCUS       DAZKLD010000003        51772 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.003, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000003 DAZKLD010000000
VERSION     DAZKLD010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 51772)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51772)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51772
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.003"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(185..310)
                     /locus_tag="ACG8KR_000146"
     CDS             complement(185..310)
                     /locus_tag="ACG8KR_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HFQ0199555.1"
                     /translation="MQKNGEKCGMTKEVVIRKVRFLNNQYYDSVKYGILWEELAD"
     gene            complement(444..1337)
                     /locus_tag="ACG8KR_000147"
     CDS             complement(444..1337)
                     /locus_tag="ACG8KR_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HFQ0199556.1"
                     /translation="MNYYSKNKENFYQKLTDDPLFSSLTDYLYEHREQETILRELKKE
                     FSQNKFSHFLDLLIDAGLIKREERRYHLNFPIFDPKDYLQQATSAAETIAEQLKRLSV
                     DEQKLAMGEVIWAYCFEDERKEAYFYGVRNSRETELLRTTAGNEKYRFITLSSIEHFP
                     LTLANYFFVQKNQLPVTKAFKELAELIGDVNESYFFDQIEVIVDRIRKNKYKNRRPSI
                     FHQSLLVTNTIKEEESFTLELPIVEKNNFEIELPTLDPSLTMEEMAFLKRQIFSELSK
                     KFIPHAFSYIKEYGTVLVSKT"
     gene            complement(1519..2445)
                     /locus_tag="ACG8KR_000148"
     CDS             complement(1519..2445)
                     /locus_tag="ACG8KR_000148"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HFQ0199557.1"
                     /translation="MSKILVFGHQNPDTDAIGAAIAFSHLQNELGKETEAVALGTPNE
                     ETQYALDHFQLSAPRVVESAKAENADVMLVDHNEFQQSISDISEVNILAVVDHHRIAN
                     FETANPLYYRAEPVGCTSTIILKLYKENQVTIPKEIAGIMLSAIISDTLLFKSPTCTP
                     EDEAAAKELAAIAEVDLNTYGLEMLKAGTNLSDKSAAVLLDLDAKSFPMGSSNVRIAQ
                     VNTVDLNEVMDRQAELEAAMEDEMKANGYDLFVLVVTNILDSDSELLVAGKGKEKVEE
                     AFQTTLTNNRALLKGVVSRKKQVVPQLTAVFS"
     gene            complement(2530..3291)
                     /gene="pflA"
                     /locus_tag="ACG8KR_000149"
     CDS             complement(2530..3291)
                     /gene="pflA"
                     /locus_tag="ACG8KR_000149"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate-lyase-activating protein"
                     /protein_id="HFQ0199558.1"
                     /translation="MEEKTIGYVHSIETFGSVDGPGLRFVVFMQGCRMRCQFCHNPDT
                     WNIGGGKEYTADELLDKAERFRPYWGDKGGITVSGGEPLLQIDFLIELFKKAKERKMH
                     TTLDTCGKPFTYEEPFFSRFQELMKYTDLLLFDIKHIDNEEHKKLTHWDNENILEMAQ
                     YLSKINKPVWIRHVLVPERSDYDEYLIRLDNFIQTLSNVDRVEILPYHTMGKYKWETL
                     GLKYPLEGIEPPTKERVENAKRLLHTEEYTKYLTR"
     gene            complement(3535..5766)
                     /gene="pflB"
                     /locus_tag="ACG8KR_000150"
     CDS             complement(3535..5766)
                     /gene="pflB"
                     /locus_tag="ACG8KR_000150"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008861 - formate C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HFQ0199559.1"
                     /translation="MEQWNGFKGTKWKEGVDTRDFIQNNYTEYRGDDSFLEGPTEATE
                     KLWDELQKLNDIQFERNGVYDMDTEIVSTITSHDAGYLDKDLEKIVGLQTDKPLKQAF
                     MPFGGINMANNALKSNGYTPNDELTHIFTDWRKTHNQGVFDAYTAEMRAARKNKIITG
                     LPDAYGRGRIIGDYRRVALYGIDYLMEQKKKDHDMTGYHTMSEEVIRLREEITEQYRA
                     LAQMKEMAAKYGYDISKPAANAKEAVQWLYFGYLAAIKSQNGAAMSIGRISAFLDIYI
                     QRDLDNGVITEKEAQELIDHLVMKLRMVKFARTPEYNQLFSGFPIWATLSIAGMGLDG
                     RSLVTKNDFRLLHTLTNMGPSPEPNLTVLYSEHLPEGFRTYASRIAIESSSIQFENDN
                     QLREQWGSDDCAIACCVSGTVVGKDMQFFGARANLAKTLLYAINGGVDEVTGAQVGPE
                     YRPITSEILDFEEVKHNFMNMMDWLAELYVNTLNIIHYMHDKYSYEAAQLALMDSDLQ
                     RTFATGIAGISHAADSLSAIKYAKVKAIRNEKGIAVDFEVDGDFPKYGNDDDRADELV
                     SWILEYFMGQIKRHKTYRNSKPTTSLLTITSNVVYGKNTGNTPDGREAGKPLAPGANP
                     SYNAEQSGLLASLNSTAKMNYGCALDGISNTQTINPSGLGKEQDTRIENLRNVLDGYF
                     SKGAYHLNVNVFSNDLLRDAMENPMKYPNLTIRVSGYAVKFRDLTREQQLDVLMRTSH
                     DRM"
     gene            complement(6043..8493)
                     /gene="parC"
                     /locus_tag="ACG8KR_000151"
     CDS             complement(6043..8493)
                     /gene="parC"
                     /locus_tag="ACG8KR_000151"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HFQ0199560.1"
                     /translation="MENRQEVQELTLEEVMGDRFGRYSKYIIQERALPDIRDGLKPVQ
                     RRILFAMNKDGNTYDKGFRKSAKSVGNIMGNYHPHGDISIYEAMVRMSQDWKLREVLI
                     EMHGNNGSMDGDPPAAMRYTEARLSKLSGEMLADIEKETVDLVWNFDDTEKEPTVLPA
                     RYPNLLVNGSTGISAGYATEIPTHNLAEVIDGTIYMIDHPQASLEKLMEYIPGPDFPT
                     GGILQGKDEIKKAYETGRGKVILRSKTKIEAMKGNKQQIVITEIPYEVNKATLVKKMD
                     EIRLNKKIDGIAEVRDESDRTGLQIVVELKKDANAQGILNYLFKNTELQINYNFNMVA
                     IDHMTPHQVGLKDILRSYIEHRKQVITKRSQFDLAKAQKRQHIVEGLMKALSILDEVI
                     ATIRESKDKKDAKKNLVDVFQFTEEQAEAIVTLQLYRLTNTDITELQKESESLIAQIT
                     ELNKILSNDKELFSVMKKELREVKKNYSSARLTTIEDEIEEIKIDTQVLVAQEDVIVS
                     VTREGYVKRTSLRSYSASKPEEIGMREGDYLLYSGELSTLDHVLLITNKANVIYRPVH
                     ELPDLKWKDAGEHISQTISNLSVDESILAVFPYQKIQAEKTFVFISKNGLIKQTRMTE
                     FEPWRTYKSRPLSCMKLKAEEDELIAVYLKEDQPGLDVFLVTHQGMGLRYPLTDVPVV
                     GAKAAGVKSMNLKDGDRIINGLLVLAEGDTPIVIVTQRGAVKRMLAQEISQTSRAKRG
                     VTVMRELKKNPHRIIYMSEGRSKEITLMNQKGQQLTIDPTDFPIGDRTSNGSFAMDEK
                     KGGEVIEVIDAPEIFIES"
     gene            complement(8505..10550)
                     /gene="parE"
                     /locus_tag="ACG8KR_000152"
     CDS             complement(8505..10550)
                     /gene="parE"
                     /locus_tag="ACG8KR_000152"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HFQ0199561.1"
                     /translation="MAKKVNNEYNDSSIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVY
                     EIVDNAVDEALSGYGSEIDVTIHEDNSITVADSGRGMPVGMHASGIPTVEVIFTVLHA
                     GGKFGQGGYKTSGGLHGVGASVVNALSKWLIVTIVRDGVEYQQKFKNGGKPDGTLKKI
                     GKTKKANGTTVHFLPDDTIFSTTKFSYEILAERLRESAFLLKGVKISLSDLRGEEPVK
                     EIFHYEEGIKEFVDYLNEEKDTLTPVVYFSGEKEGIEVEVAYQYNDGYSENVLSFVNN
                     VRTKDGGTHEAGMKAAMTKSYNEYARKVGLLKERDKNLEGSDFREGLAAVLSIRVPEN
                     LLQFEGQTKEKLGTPVARTVVDNVISEQMGFYLQENSEMSQMLVRKAIKAREAREAAR
                     KAREESRNGKKRKKGESLLSGKLTPAQSRNPKKNELYLVEGDSAGGSAKQGRDRKFQA
                     ILPLRGKVINTEKAKMQDILKNEEINTMIYTIGAGVGPEFSIEDCNYDKVIIMTDADT
                     DGAHIQVLLLTFFYRYMKPLIEAGKVYIALPPLYKVSKGQGKKQVIEYAWTDDELAAM
                     IKKVGKGYMLQRYKGLGEMNAEQLWETTMDPTSRTLIRVRIDDAAQAERRVTTLMGDK
                     VEPRRKWIENHVQFTLEEDGSILDKKEDTEISPSVSNDLLDEERADKNENNQLFEVE"
     gene            complement(10716..11150)
                     /locus_tag="ACG8KR_000153"
     CDS             complement(10716..11150)
                     /locus_tag="ACG8KR_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HFQ0199562.1"
                     /translation="MSVKNPTQETIFNYLEEAKNIAVVGLSNKPERTSYMVAQVLQDN
                     GYKIIPVNPLLAGEEILGEKVYANLTEIDDPIDIVDVFRRSEFLPEIAGDFLKTDAKV
                     FWAQLGIENKEAAEMLRKAGRTDIVMDRCIKIELGKLKENQK"
     gene            11277..11915
                     /gene="plsY"
                     /locus_tag="ACG8KR_000154"
     CDS             11277..11915
                     /gene="plsY"
                     /locus_tag="ACG8KR_000154"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HFQ0199563.1"
                     /translation="MKLILLLILAYLLGSIPSGVWIGKLFFKKDIRQHGSGNTGTTNT
                     FRVLGKTAGIVVLLMDILKGTLATCLPMIFHLSGINPLWFGVCAILGHTFPIFAGFKG
                     GKAVATSAGMLLGFNPVFFVYSCIIFIVSLFCTSMVSLSSMISAVLITLSTIVLPYVA
                     PVILAKPNWLLTIIAFLVSSFIFYRHKDNIKRIRNHTESRIPFGLNSSKKKS"
     gene            complement(12047..12922)
                     /locus_tag="ACG8KR_000155"
     CDS             complement(12047..12922)
                     /locus_tag="ACG8KR_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HFQ0199564.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNRELFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADTVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
     gene            complement(13004..13795)
                     /gene="codY"
                     /locus_tag="ACG8KR_000156"
     CDS             complement(13004..13795)
                     /gene="codY"
                     /locus_tag="ACG8KR_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HFQ0199565.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            complement(13813..15213)
                     /gene="hslU"
                     /locus_tag="ACG8KR_000157"
     CDS             complement(13813..15213)
                     /gene="hslU"
                     /locus_tag="ACG8KR_000157"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002409948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HFQ0199566.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALVKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            complement(15227..15775)
                     /gene="hslV"
                     /locus_tag="ACG8KR_000158"
     CDS             complement(15227..15775)
                     /gene="hslV"
                     /locus_tag="ACG8KR_000158"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HslVU peptidase proteolytic subunit"
                     /protein_id="HFQ0199567.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            complement(15812..16717)
                     /gene="xerC"
                     /locus_tag="ACG8KR_000159"
     CDS             complement(15812..16717)
                     /gene="xerC"
                     /locus_tag="ACG8KR_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HFQ0199568.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
     gene            complement(16915..18291)
                     /gene="trmFO"
                     /locus_tag="ACG8KR_000160"
     CDS             complement(16915..18291)
                     /gene="trmFO"
                     /locus_tag="ACG8KR_000160"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0047151 - tRNA
                     (uracil(54)-C5)-methyltransferase activity,
                     5,10-methylenetetrahydrofolate-dependent [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HFQ0199569.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLNSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
     gene            complement(18260..20338)
                     /gene="topA"
                     /locus_tag="ACG8KR_000161"
     CDS             complement(18260..20338)
                     /gene="topA"
                     /locus_tag="ACG8KR_000161"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HFQ0199570.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHGHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDVVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            complement(20460..21317)
                     /gene="dprA"
                     /locus_tag="ACG8KR_000162"
     CDS             complement(20460..21317)
                     /gene="dprA"
                     /locus_tag="ACG8KR_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HFQ0199571.1"
                     /translation="MYQIEENLLKLAYTKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITKHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVIEAGYVIVSGCAKGIDTYGHQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHCFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            complement(21373..22140)
                     /locus_tag="ACG8KR_000163"
     CDS             complement(21373..22140)
                     /locus_tag="ACG8KR_000163"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HFQ0199572.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDARSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            complement(22133..22993)
                     /gene="ylqF"
                     /locus_tag="ACG8KR_000164"
     CDS             complement(22133..22993)
                     /gene="ylqF"
                     /locus_tag="ACG8KR_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HFQ0199573.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
     gene            complement(23087..24046)
                     /locus_tag="ACG8KR_000165"
     CDS             complement(23087..24046)
                     /locus_tag="ACG8KR_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFQ0199574.1"
                     /translation="MTYTHLTTDELVMIESYFKINQSVAKTAHFLNRSRQTIHKVYLF
                     FKQGKSALEYYQQYKKNKSNCGRRPLVLPEEQSEYIQRKVVQGWTPDVIVGRAAFPIR
                     CSARTIYRMFKKGLFNPSDLPMKGKRKPNGHQERRGKQAFRRSIHEREKDYSQFSNEF
                     GHLEGDTIVGLKHKSAVITLVERLSKVIITLKPCGRQAIDIENKLNHWFESVPKNLFK
                     SITFDCGKEFSNWKQISNANDIAIYFADPGTPSQRGLNENSNGLLRRDGLLKSMDFNS
                     VDESFIQSVASKRNNIPRKSLNYRTPLEVFLSYVSIDDLSNLI"
     gene            24318..24716
                     /locus_tag="ACG8KR_000166"
     CDS             24318..24716
                     /locus_tag="ACG8KR_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFQ0199575.1"
                     /translation="MNSMVFVNFPVADVNRSVAFYEKLGFKKNEEFSTEEAGSMVWDD
                     TFWIMLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQIAKENGGTFHE
                     VDMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
     gene            24953..25495
                     /locus_tag="ACG8KR_000167"
     CDS             24953..25495
                     /locus_tag="ACG8KR_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HFQ0199576.1"
                     /translation="MNTLIAKDVFRLPEMNARLLSTSALLLALDVILYKIAIGPAYFQ
                     ITFGFLSMALMGYLYGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLF
                     LYHKKINLPSVIAAQLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQI
                     MVVYSFIKTLEARKVFKRAV"
     gene            complement(25578..26129)
                     /gene="lepB"
                     /locus_tag="ACG8KR_000168"
     CDS             complement(25578..26129)
                     /gene="lepB"
                     /locus_tag="ACG8KR_000168"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFQ0199577.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(26281..26508)
                     /locus_tag="ACG8KR_000169"
     CDS             complement(26281..26508)
                     /locus_tag="ACG8KR_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HFQ0199578.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            complement(26505..27023)
                     /gene="msrA"
                     /locus_tag="ACG8KR_000170"
     CDS             complement(26505..27023)
                     /gene="msrA"
                     /locus_tag="ACG8KR_000170"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFQ0199579.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            complement(27043..27756)
                     /locus_tag="ACG8KR_000171"
     CDS             complement(27043..27756)
                     /locus_tag="ACG8KR_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HFQ0199580.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            complement(27759..28655)
                     /locus_tag="ACG8KR_000172"
     CDS             complement(27759..28655)
                     /locus_tag="ACG8KR_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HFQ0199581.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            complement(28813..29655)
                     /locus_tag="ACG8KR_000173"
     CDS             complement(28813..29655)
                     /locus_tag="ACG8KR_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFQ0199582.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            29787..30440
                     /locus_tag="ACG8KR_000174"
     CDS             29787..30440
                     /locus_tag="ACG8KR_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HFQ0199583.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            complement(30503..31030)
                     /locus_tag="ACG8KR_000175"
     CDS             complement(30503..31030)
                     /locus_tag="ACG8KR_000175"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HFQ0199584.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(31050..31997)
                     /locus_tag="ACG8KR_000176"
     CDS             complement(31050..31997)
                     /locus_tag="ACG8KR_000176"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HFQ0199585.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            complement(32009..33892)
                     /locus_tag="ACG8KR_000177"
     CDS             complement(32009..33892)
                     /locus_tag="ACG8KR_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFQ0199586.1"
                     /translation="MKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKTS
                     LLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVRA
                     YEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIST
                     LSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDRY
                     FLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMRA
                     GVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRINDQ
                     TLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQE
                     NEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEKR
                     RLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVAD
                     KLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKEW
                     ETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSEW
                     VED"
     gene            complement(34055..36133)
                     /locus_tag="ACG8KR_000178"
     CDS             complement(34055..36133)
                     /locus_tag="ACG8KR_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HFQ0199587.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKTSPIK"
     gene            complement(36146..36421)
                     /locus_tag="ACG8KR_000179"
     CDS             complement(36146..36421)
                     /locus_tag="ACG8KR_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0199588.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            complement(36783..37238)
                     /locus_tag="ACG8KR_000180"
     CDS             complement(36783..37238)
                     /locus_tag="ACG8KR_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0199589.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
     gene            complement(37392..38600)
                     /locus_tag="ACG8KR_000181"
     CDS             complement(37392..38600)
                     /locus_tag="ACG8KR_000181"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001680 - tRNA 3'-terminal CCA addition
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HFQ0199590.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNERAAGIH
                     AFIETECYICCPGLKEEGEALLRFAELPSVPLKEESQAWALLISQLDLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LDFLSKEKLI"
     gene            complement(38712..39044)
                     /locus_tag="ACG8KR_000182"
     CDS             complement(38712..39044)
                     /locus_tag="ACG8KR_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HFQ0199591.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            39192..40067
                     /locus_tag="ACG8KR_000183"
     CDS             39192..40067
                     /locus_tag="ACG8KR_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFQ0199592.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSSELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
     gene            complement(40149..40730)
                     /locus_tag="ACG8KR_000184"
     CDS             complement(40149..40730)
                     /locus_tag="ACG8KR_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ReoY family proteolytic degradation factor"
                     /protein_id="HFQ0199593.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
     gene            complement(40736..41995)
                     /locus_tag="ACG8KR_000185"
     CDS             complement(40736..41995)
                     /locus_tag="ACG8KR_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFQ0199594.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIVPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELY
                     HLASENAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQME
                     ETANPYAEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRA
                     QELLTYYLTLEPGDMEALSLLDDLTER"
     gene            complement(41997..43043)
                     /locus_tag="ACG8KR_000186"
     CDS             complement(41997..43043)
                     /locus_tag="ACG8KR_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0199595.1"
                     /translation="MELDYQVEDALTKVFYGETLVSQLRELSIDQPILFLTNQRYYDL
                     FADKINPLFANQANNDWYICANTQCNHLTELKNLLDFVKRYPENQSMFIIGFGNEGIM
                     DLAGFFQKHTTLDSSLWMIPVSIRSLSRALTPQRTILQLPNRVVLQTINLPERIVYDQ
                     TISERQVDGKQIDFLTFICCGMICDHRFLQNLYKNYPNKKQLTTRPFAGMIEEMIYFY
                     QLEAENLSNYGKLFEQAFYLTENGHLLSASMKKFLGMMFHFVWNLQAYPFDFQLKNFF
                     VWLDLLGYPIHWLEQISKMEYLEKVLFLAEKSKKILVLEKIGIIDGYQHPNEKELLRT
                     MTVFQQIVSEIRGN"
     gene            complement(43278..43553)
                     /locus_tag="ACG8KR_000187"
     CDS             complement(43278..43553)
                     /locus_tag="ACG8KR_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HFQ0199596.1"
                     /translation="MANKAELIEKVASATDLTKKDATAAVDAVFSTIQDALANGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGEEIEIPASKVPAFKPGKALKDAVK"
     gene            complement(43796..45106)
                     /gene="der"
                     /locus_tag="ACG8KR_000188"
     CDS             complement(43796..45106)
                     /gene="der"
                     /locus_tag="ACG8KR_000188"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HFQ0199597.1"
                     /translation="MANPTIAIVGRPNVGKSTIFNRIAGERISIVEDTPGVTRDRIYA
                     KGEWLGREFSVIDTGGIDLGDEPFMDQIKHQAEIAIEEADVIICVVSGREGVTDADEM
                     VAKILYRSNKPVILAVNKVDNPEMRNDIYEFYSLGLGDPYPVSGSHGLGIGDILDEAV
                     KYFSEETEEEDDDTIKFSLIGRPNVGKSSLINAILGEERVIVSDIEGTTRDAIDTHFV
                     SESGQKFLMIDTAGMRKRGKVYENTEKYSVMRAMRAIDRSDIVLMVLNAEEGIREQDK
                     RVAGYAHEAGRGIIIVVNKWDLVKKETNTMRDFEQEIRDEFRYLDYAPIVFVSAVTKQ
                     RLERLPEMIEQVSMNQNLRISSAVLNDIIMDAVAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYERFLENQIRKAFTFEGTSIRIIPRRRK"
     gene            complement(45288..46517)
                     /gene="rpsA"
                     /locus_tag="ACG8KR_000189"
     CDS             complement(45288..46517)
                     /gene="rpsA"
                     /locus_tag="ACG8KR_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HFQ0199598.1"
                     /translation="MTEFDQNQENNQSMEDAMKSVQEVKVGDVVKGEVLVIEDKQVIV
                     GIEGAGVEGVVPAKELSTLPVEDISEVVKVGDVLDLVVISTIGKDKENGSYLLSKRRL
                     DAKKVWEDIERDFQEGKVIEAPVTNVVKGGLVVDVGVRGFVPASMVEDHFVADFSDYK
                     GKTLSFKIIEIEPSENRLILSHKAVVENEREEKKKEILSTIHEGDVLSGRVARLTDFG
                     AFVDLGGIDGLVHVSEISHSHVAKPSDVLAVNDDVNVKVLSINPEQERVSLSIKDTQP
                     GPWSEIDTKAPVGTVLDGTVKRLTSFGAFVEVFPGVEGLVHISQISHKHIATPHEVLH
                     EGDEVKVKVLEIHPEEHRIALSIKALEEKPASEEEPKNVESYELPEENTGFTMGDILG
                     DALKEEVEEKATDDSEK"
     gene            complement(46652..47332)
                     /gene="cmk"
                     /locus_tag="ACG8KR_000190"
     CDS             complement(46652..47332)
                     /gene="cmk"
                     /locus_tag="ACG8KR_000190"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - (d)CMP kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HFQ0199599.1"
                     /translation="MTQISIAIDGPASSGKSTVAKILAKELNYIYTDTGAMYRAVTYL
                     AITNNVSFSDEEGLVSLIKKYPISFSQQEDGQHVFIASKDVTLEIRQPDVTKAVSEVS
                     AHGKVREELVAVQRKIGERGGVVMDGRDIGTAVLPNAEVKIFLVASVSERAERRFKEN
                     QEKGIPTDLETLTKEIQERDHYDSTRDVSPLKQAEDAVRIDTTGKSIPEVVAAIKAVV
                     LKKGYLLF"
     gene            complement(47381..48016)
                     /locus_tag="ACG8KR_000191"
     CDS             complement(47381..48016)
                     /locus_tag="ACG8KR_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAG1386/EF1546 family surface-associated
                     protein"
                     /protein_id="HFQ0199600.1"
                     /translation="MSRKDRHQSTENNETQEPWEQPIYDTDDEVSSRSSQRQQKKGNT
                     LFLSLFLILLVLCIAIPAGAYFWIRNGSSNNATAATSSETSTSVVESSTSTTKEESTE
                     TSTVESSTIAEESTVSQDTGVTSESSIAAETTPTSSSEATYQAGDSSSNAAETTPSSS
                     AAAGSTTTVQAGEGPKQVAERAGITTDQLFELNGLDPNNFMLYPGQELRIK"
     gene            complement(48082..49485)
                     /locus_tag="ACG8KR_000192"
     CDS             complement(48082..49485)
                     /locus_tag="ACG8KR_000192"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043138 - 3'-5' DNA helicase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HFQ0199601.1"
                     /translation="MLINELKKYFGFDSFRPGQEEIIQALLDGEDTLAILPTGTGKSL
                     CYQLTGYLMEGLVIIVSPLLSLMEDQVTQLQKRGEKRVAAFNSLLSQSERRYVLRHLS
                     QYKFLFLSPEMLTNPSLLEHLKKQNIALYVVDEAHCVSQWGVDFRPEYQQLGKIRKLL
                     GNPVTLALTATATDLVARDIRHVLFDRPPKEIRQSVNRQNISLFVRKTQQKEQELEHF
                     MERAQGAAIIYCATKKEVERLYHLFRERFTVGYYHGGLDAAQRRQLQQQFVKNQLQFL
                     IATNAFGMGIDKSDIRYVVHYDLPDSLENYVQEIGRAGRDQEESAAILFYQSGDERIH
                     YFFNQLSREQRQSFELYLEYATKQAPFDELQKKWMELIQQSEKPENWVERLKRQEKEK
                     EFRLQQMLRYINEENCRRKFILAYFGEKLSEKPQNCCDIDGAKAELLPKRKLFSKTEQ
                     LHWETILLNLFKKNMEH"
     gene            complement(49472..50503)
                     /locus_tag="ACG8KR_000193"
     CDS             complement(49472..50503)
                     /locus_tag="ACG8KR_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0199602.1"
                     /translation="MIVFILSLFSSGHKLRISSLYQLLVGKRTTSVLIYGFTHELLFI
                     HNSFPELKQDKFYQILQKIAQQGWIEINENEAKLTPAGADMLSEHQIEHTGLRFDRYG
                     RTGETSWRLIKFAVQVISNLATGIQDYLPAETSPFYTFQLKKWLSESQLPREILIDTA
                     YESLVQLFSEIPEEAADFLANQLSGNERTGLLPYQLAKNNDESEVYLQQSRCIHLLLA
                     QIEKRPDSLWHALIDSLLQQNFNQSMMITKQMFMNGQTIDQIMAIRHLKRGTVTDHLI
                     EWALFFDDFPYEWILSAETIERLEPNKDSVREWRFSEWNVDGQLDYGEFRLYQIYLLR
                     KEAIQNVNK"
     gene            50534..50755
                     /locus_tag="ACG8KR_000194"
     CDS             50534..50755
                     /locus_tag="ACG8KR_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HFQ0199603.1"
                     /translation="MSLLCKLVPERCIACGLCQIEAPDIFDYDEEGIVLFAQEPEAHQ
                     QFVMPEEETYVKKAYQKCPVRAILLENKI"
     gene            complement(50920..51657)
                     /locus_tag="ACG8KR_000195"
     CDS             complement(50920..51657)
                     /locus_tag="ACG8KR_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HFQ0199604.1"
                     /translation="MKIFRRILIGLVIILGLLGGAGYYYIQQNTYEPSSEAQVLVKEG
                     KNKEDYLFFEGEKKNHGKSPLLIFYPGALVEPESYSIWAQDLAKNGFSVAIVKMPLDL
                     AVMGGNKADKVKEDFPNSDYVIGGHSLGGVMASRYVNKNHSDKHLKGVFFMASYPDKK
                     GTLTESAVPVLSITATKDKVLNQEAYQEAKQYLPERTEYVSINGGNHGGFGSYGEQKG
                     DGEAEISNADQQTVIVNTLENWLKKLK"
BASE COUNT        14074 a        10892 c         8864 g        17942 t
ORIGIN      
        1 tttatgtggc aaagaaaaaa caaacaaaat aaaaagcatt ttgaagcctg actattacga
       61 cttcataaat gcttttttag ttttaaacac ttcaatctaa aagaataacc caagattgga
      121 taaattatta atgactcgcc attcgtttct tagtgattct ggattatcaa atggacttcc
      181 taatctaatc agctagttct tcccataaaa tgccatactt gactgaatca taatattgat
      241 tatttaaaaa tctaactttt cttatcacta cttcctttgt cattccacat ttctcaccat
      301 ttttttgcat tcctttgttt ccagaccatg tagtgaatct aataggtggt agataggcaa
      361 aatactcaaa taattctttt aaccataaag gaagagaaag gctcctcctg caagagtcag
      421 tataattttt gcttactgct ctcttaggtt ttactcacta aaactgttcc atattctttg
      481 atataagaaa atgcatgcgg tataaatttc ttgctcagct cagaaaagat ttgtcgttta
      541 agaaaggcca tttcctccat tgtcaatgaa ggatccaatg ttggtaattc aatctcaaaa
      601 ttgttttttt cgacaatagg taattctaat gtgaatgatt cttcttcctt tatagtattc
      661 gtcacaagta gagactgatg gaaaatacta ggccggcgat ttttatattt attcttacgt
      721 atcctgtcaa cgataacttc tatttgatca aaaaagtacg attcatttac gtctccaata
      781 agttcggcta attctttgaa tgccttcgtt acaggaagct gattcttctg tacaaaaaag
      841 taattagcca aagtaagcgg gaagtgttct atagaggaaa gtgtgataaa acggtacttc
      901 tcatttcctg cagttgtcct cagcaattct gtctctcggc tgtttcttac tccataaaaa
      961 tacgcttctt tccgttcatc ttcgaaacaa tacgcccaaa taacttctcc catcgctaat
     1021 ttttgttcat caacagagag tctcttcaat tgttcagcta tggtttcagc agccgaagtg
     1081 gcttgttgca agtaatcttt aggatcaaat attggaaagt tcaaatgata tcttcgttct
     1141 tccctcttga ttaatcctgc atcgatcaat aaatcaagaa aatgggaaaa tttattttgc
     1201 gaaaattctt ttttcaattc tcttagaatc gtttcttgtt ctcgatgttc atatagataa
     1261 tcggtcaatg aagaaaaaag cggatcatcg gtcagttttt gataaaagtt ttccttattt
     1321 tttgaataat agttcatata tgttctcttt tctatccgtt ttattttact taaaaggctt
     1381 ttacgtaaaa aggaggaaga acaagagtca taattgactg ttattctcct ccatttcttc
     1441 ggcctttagt atgttctatg tttcttttta tatcaatcgc ttatccattc attttgaatg
     1501 cttcatacta ccatttgatt agctaaatac agccgtcaat tgaggtacga cttgtttttt
     1561 acgtgaaacg acacctttaa gcaaagcacg gttgttggtc aaagttgttt ggaaagcttc
     1621 ttctactttt tcttttcctt taccagcaac aagtaattct gaatcactgt ctaagatgtt
     1681 tgtgacaact aatacgaaca gatcgtagcc atttgctttc atttcatctt ccattgctgc
     1741 ttctagctct gcttgtcgat ccatcacttc attaaggtcc actgtattga cttgagcgat
     1801 acgaacgttg gaactgccca ttgggaaact ttttgcatcc aaatcaagaa gaaccgcagc
     1861 tgatttatca cttagatttg ttcctgcttt caacatttca agtccataag tgtttagatc
     1921 gacttcagcg attgctgcaa gttcttttgc agctgcttca tcttcaggcg tacatgttgg
     1981 tgatttgaac aacaatgtat ctgaaatgat agctgagagc atgattcctg cgatttcttt
     2041 aggaattgtt acttggtttt ctttatatag ttttaaaata atcgtacttg tacatccaac
     2101 tggttctgca cgatagtaca atggattagc tgtttcaaag tttgcaatac gatggtggtc
     2161 tactacagct aaaatattta cttcgctgat atctgaaata ctttgttgga attcattatg
     2221 atccacaagc atgacatctg cattttctgc tttagctgac tctactactc gtggtgcaga
     2281 tagctgaaag tggtctaatg cgtattgtgt ttcttcatta ggtgtaccta gagcaactgc
     2341 ttctgtttct ttacctaatt cattttgcaa gtgtgaaaac gcaatggctg ccccgattgc
     2401 atctgtatct ggattttggt gaccaaaaac taaaattttt gacatgtcat tcgctccttt
     2461 gtgatttctc ctatttaata atagcaaaaa aacggaaaga agcaatactt atcttctttc
     2521 cgatgcgacc tatcgcgtta aatattttgt atactcttct gtatgaagca atcgtttagc
     2581 attttcgaca cgttcttttg taggtggttc gatcccttct aatggatatt tcaaaccaag
     2641 tgtttcccac ttgtattttc ccatcgtatg ataaggaaga atctctactc tgtctacatt
     2701 tgacaaggtt tgaataaagt tgtctagtcg gatcaaatat tcatcataat cacttcgctc
     2761 aggaactagt acatggcgaa tccaaaccgg tttgttgatt tttgacaaat attgagccat
     2821 ttctaaaata ttttcattgt cccaatgcgt tagtttttta tgctcttcat tatcgatgtg
     2881 tttgatgtca aataacagta aatctgtata cttcatcaat tcttgaaaac gtgaaaagaa
     2941 aggttcttca tacgtaaaag gttttccgca tgtatccaat gtagtatgca tcttccgctc
     3001 ttttgctttt ttaaatagtt cgatcaaaaa atcgatctgc aatagaggtt ctccaccact
     3061 aacagtaata ccacctttat ctccccagta aggtctgaag cgttctgctt tatctaaaag
     3121 ttcatctgct gtgtattctt tccctccgcc aatattccaa gtatctggat tgtgacaaaa
     3181 ttggcaacgc atgcggcatc cttgcataaa cacgacaaag cgcagtccag gtccgtctac
     3241 tgaaccgaaa gtttcgatag aatgaacata gccgattgtt ttttcttcca tttaagttca
     3301 catcttttct ttgatttcat tttaagtaca agttagctgt cgaagcgaag ctttctgaac
     3361 aaaatttttc gcaattgccg cttcgttatg tgtaaaattt acagggacgg aacatacaag
     3421 tcctgcccct gtaaatcgtt tctttcaaat aagcatactg attattcgat tttgatgcga
     3481 tggctattca acgtcctttc gacgctaagt cgaaaaactt ttttcacgaa ctttttacat
     3541 tctgtcgtgt gaagttctca tcaagacatc taattgttgt tctcttgtca agtcacggaa
     3601 tttaactgca taaccagata cccggatcgt caagttcgga tatttcattg gattttccat
     3661 tgcatcacgc aataaatcat tactgaatac attgacattc aaatgataag ctccttttga
     3721 gaagtatcca tctaagacat tacgcaagtt ttcgattctt gtatcttgtt cttttccaag
     3781 acctgaagga ttgattgttt gggtatttga aattccatcc aatgcacagc cgtaattcat
     3841 tttagctgtt gagttcaggc tagctaataa cccactttgt tccgcattgt aacttggatt
     3901 tgctcctggc gcaagtggtt ttccagcttc acggccatca ggagtatttc ctgtattttt
     3961 accgtaaaca acatttgaag taatcgtcaa taatgaagtt gttggttttg agttgcggta
     4021 agttttatga cgtttgattt gtcccatgaa gtattccaag atccatgaaa ctaattcatc
     4081 tgcacggtca tcatcatttc cgtatttagg gaagtcgcca tccacttcaa aatcaactgc
     4141 tattcctttt tcattacgga ttgcttttac ttttgcatat ttgatcgctg acaagctgtc
     4201 tgccgcatga gagatcccag caatacctgt agcaaatgtg cgttgcaagt cagaatccat
     4261 caaagccagc tgagctgctt cataagaata tttatcatgc atataatgaa tgatgttcaa
     4321 cgtattgaca tatagttctg ctaaccaatc catcatattc atgaagttat gtttcacttc
     4381 ttcaaagtct agtatttctg atgtgattgg acgatattct ggtccaactt gggcgccagt
     4441 cacttcatcg actccgccat tgatggcata taatagtgtt ttagccaagt tagcacgagc
     4501 gccaaagaat tgcatgtctt ttccgacaac tgttcctgat acacagcaag caatcgcaca
     4561 atcatcagaa ccccattgtt cacgaagctg attatcattt tcgaattgaa tcgaagaact
     4621 ttcgatagcg attctgctag catatgttct gaatccttct ggaaggtgtt cagaatacaa
     4681 aacagtcaag tttggttctg gtgaaggtcc catatttgtt aatgtatgaa gcagacggaa
     4741 atcattcttt gtaactaatg aacgaccatc taatcccata cctgcaatgg ataaagtagc
     4801 ccagataggg aatcctgaga ataattggtt atattcaggt gtacgcgcga atttgaccat
     4861 acgtaatttc attactaagt ggtcgatcaa ttcttgtgct tctttttctg tgattactcc
     4921 attatccaaa tcacgttgga tatagatatc aaggaatgct gagatacgtc cgattgacat
     4981 cgcagcacca ttttgtgatt tgattgctgc taaatatccg aagtatagcc attggacagc
     5041 ttcttttgca tttgcagctg gtttagaaat atcatagcca tatttcgcag ccatttcttt
     5101 catttgcgct aatgcacgat attgttcagt gatttcttca cgtaaacgaa tcacttcttc
     5161 actcattgta tgataaccag tcatatcatg atcttttttc ttttgttcca tcaaatagtc
     5221 gattccatac aatgccacac gacgatagtc accgatgata cgtccgcgac cgtaagcgtc
     5281 tggaagccct gtaatgattt tgtttttacg agcagcacgc atttcagcag tataagcgtc
     5341 aaatacacct tgattatgtg ttttgcgcca gtcagtgaaa atatgtgtca attcatcgtt
     5401 tggtgtatag ccattgcttt ttagcgcatt gttagccata ttgattccgc cgaacggcat
     5461 gaatgcttgt ttcaaaggtt tgtctgtctg tagaccgacg attttttcca aatctttatc
     5521 caaatagcct gcatcgtgtg aagtaatagt tgatacgatt tctgtgtcca tatcatagac
     5581 accgttacgc tcgaattgga tatcatttaa cttttgcaat tcatcccata atttttctgt
     5641 tgcttctgtt gggccttcca aaaagctgtc atcaccgcga tattcagtgt agttattttg
     5701 aataaagtct ctagtgtcta cgccttcttt ccatttggta cctttaaatc cattccattg
     5761 ctccattgta tggacctcct aaatttctgt tttatgtaac agtttttacg tgattagtat
     5821 aacacagcac cctatctttg caagggtttt cttgtttttt atttaacaat ttttatgttt
     5881 tatttacata tatttaaatc aacgttgaca taccaacatt taagcattgt gaacactttc
     5941 atcttttttt gcaaacgtat aaggatttat taaaaatgaa tgaaaaagag acgccacctg
     6001 tattaataca gatggcgtct cttcattaaa atactacaac agttacgact cgatgaaaat
     6061 ctcaggagca tcgataactt cgatgacttc tccacctttt ttctcatcca tcgcaaatga
     6121 tccattcgat gttcgatcac ctatagggaa atcagttggg tcaatcgtta actgctgccc
     6181 tttttgattc atcaaggtta tttcttttga tcttccttcc gacatataga taatacggtg
     6241 cggatttttt ttcagctcac gcatgaccgt cactccccgc ttcgcacgtg aagtttggct
     6301 gatttcttga gcaagcatgc gtttaacagc tcctcgctga gtaacgatta cgatcggcgt
     6361 atcaccttct gctaacacaa gcagcccgtt gataatccta tctccatctt tcaaattcat
     6421 agacttcact ccagctgcct ttgctccaac aacgggaaca tctgttaaag gatagcgcaa
     6481 acccattcct tgatgcgtta ctaagaaaac atctaagcct ggctgatctt ctttcagata
     6541 cacagcaatc agttcatctt cttccgcctt tagcttcata cagctgagcg gacggctctt
     6601 ataggtgcgc catggttcga actctgtcat tcgtgtttgc ttgatcaatc cattcttgct
     6661 gatgaataca aatgttttct ctgcttgaat tttttgatat gggaaaacag ctaaaatcga
     6721 ttcgtctaca gacagattgg agattgtttg agaaatgtgc tcaccagcat ctttccattt
     6781 caaatcgggt agttcatgga cagggcggta gatcacattt gctttatttg tgatcagaag
     6841 aacatggtcc aatgtactca attcacctga gtatagcaag tagtcgcctt ctcgcatccc
     6901 gatttcttct ggtttggaag cactataaga acgaaggctt gttcgcttca catagccttc
     6961 tcgcgtcaca ctgacaatta cgtcttcttg tgccactaag acttgcgtat caattttgat
     7021 ttcttcgatt tcgtcttcga ttgtcgttag acgagcggaa gaataatttt ttttcacttc
     7081 tcgcaattct tttttcataa cagagaaaag ctctttgtca tttgaaagga ttttattcaa
     7141 ctctgtgatt tgcgcaatca agctttcgga ttccttttga agctctgtga tatccgtgtt
     7201 cgtcaaacgg tataattgca atgtgacaat tgcttctgct tgttcttctg taaattgaaa
     7261 gacgtcgact aaattctttt tggcgtcttt tttgtcttta ctttcacgaa tcgttgcgat
     7321 tacttcatcc agaatcgata aagctttcat caatccttct acgatatgct gtcttttttg
     7381 agctttggct aaatcgaatt gactacgttt tgttatgact tgctttcgat gctcgatata
     7441 acttcttaaa atgtctttca gtccaacttg atgcggagtc atatggtcga tagcgaccat
     7501 attgaaattg taattgatct gtagctcagt gtttttgaac aaataattca aaattccttg
     7561 agcattggcg tcttttttca attcgacgac aatttgcaga cctgttctat cactttcatc
     7621 tcgtacttca gctattccat cgattttctt attcaaacgg atctcatcca tttttttcac
     7681 caaggtcgct ttatttactt catatgggat ttctgtgatc acgatttgct gtttattacc
     7741 cttcattgcc tctatctttg ttttcgaacg aagaataact tttcctcgtc ctgtttcata
     7801 agcttttttg atttcatctt ttccttgcaa gatccctcct gttggaaagt caggtccagg
     7861 aatatattcc atcaattttt caaggctcgc ttgtggatga tcgatcatat agattgtgcc
     7921 atcgatcact tcagctaagt tatgagtcgg gatttctgtt gcatatccag cagatatacc
     7981 tgtcgatcca ttgacgagaa ggtttgggta tcgagctgga aggactgttg gttctttttc
     8041 tgtatcatca aaattccaca cgagatcgac tgtttctttc tcgatatctg ctagcatctc
     8101 tccacttaac ttggataatc tggcttcagt ataacgcatc gcagcaggtg gatcaccgtc
     8161 catactcccg ttattcccgt gcatttcgat cagtacttcc cgcaacttcc agtcttggct
     8221 catgcggacc atcgcttcat aaatactgat gtcaccatga ggatgataat tccccatgat
     8281 atttccgaca gattttgctg atttacggaa tcctttgtca tacgtattgc catctttgtt
     8341 catggcaaat aatatccggc gttgcactgg cttcaatcca tcacggatat caggcagtgc
     8401 acgttcttgg ataatatatt tggaataacg tccgaagcgg tcacccatca cttcttctaa
     8461 agttaattct tgtacttctt gtcgattttc catcaggtga cctcctattc tacttcaaat
     8521 aactgattgt tttcattttt atctgctcgt tcttcatcta ataaatcatt agaaactgaa
     8581 ggagagattt cagtatcctc ttttttatct aagatactgc catcttcttc gagcgtaaat
     8641 tgcacgtgat tttcgatcca ttttcttcga ggttctactt tgtcacccat cagtgtggtt
     8701 actcgacgtt cagcttgcgc agcatcatcg atacggacgc ggatcaacgt acgggaagtc
     8761 ggatccatcg ttgtttccca caattgttcc gcattcatct ctccaagtcc tttatatcgc
     8821 tgcagcatat atcctttgcc gactttttta atcattgctg ccaattcgtc atctgtccac
     8881 gcatattcaa tgacttgctt ctttccttgt cctttggata ctttatataa aggcggtaac
     8941 gcgatatata ctttcccagc ttcaatcagc ggtttcatat aacgataaaa gaaagtaagc
     9001 agtaatactt gaatatgggc gccgtctgta tccgcatcgg tcatgatgat tactttgtcg
     9061 taattacagt cttcgataga aaactcagga ccgactcctg caccaatcgt atagatcatc
     9121 gtattgattt cttcattttt caagatatct tgcattttag ctttttcggt attgatgact
     9181 tttcctcgta aaggtagaat tgcttggaac tttctgtctc ttccttgttt agcagatccg
     9241 ccagcagaat ccccttcgac taaatacaat tcatttttct tagggtttcg ggattgtgcg
     9301 ggtgtcaatt ttcctgaaag aagagattcg ccttttttac gcttcttccc atttcggctt
     9361 tcttctctgg ctttacgtgc tgcttctctc gcttctctgg ctttgattgc tttacggacc
     9421 aacatctggc tcatttcgct attttcctgc aaataaaagc ccatttgttc actgatgacg
     9481 ttatctacta cggtacgagc aactggcgta ccaagttttt cttttgtttg cccttcaaat
     9541 tgcagaagat tttcaggtac acggatagaa agtacagcag ctaacccttc tctgaaatca
     9601 ctaccttcca gattcttgtc ccgttctttc agcaaaccaa cttttctcgc atattcatta
     9661 taagatttcg tcatcgccgc tttcatacca gcttcatggg ttcccccatc ttttgtccga
     9721 acgttattga caaaagataa aacattttct gaataaccgt cattgtattg ataagcgacc
     9781 tctacttcga taccttcttt ttctcctgaa aaataaacaa ctggtgtcaa tgtatctttt
     9841 tcttcattca gataatcaac aaattctttg attccttctt cgtaatgaaa aatttccttg
     9901 actggttctt ctccacgaag atcagaaaga gagattttaa cgccttttag aagaaaggca
     9961 gattctctta accgttcggc taaaatttca tatgaaaatt tcgttgtact gaatattgta
    10021 tcatctggta aaaaatggac agtcgtcccg tttgcttttt ttgtttttcc tatctttttc
    10081 aaagtaccgt caggtttacc gccgttttta aatttctgct gatactctac gccatctcta
    10141 acgatcgtta caattagcca tttcgataag gcatttacca cactggctcc tacaccgtgc
    10201 agtccaccag aagttttgta ccctccttga ccaaactttc cgccagcatg cagcacagta
    10261 aaaatcactt caactgttgg tattccggaa gcatgcattc ctacaggcat cccacgtccg
    10321 ctatctgcaa cagttatact attgtcttca tggatggtca catcaatctc actgccataa
    10381 cctgataatg cttcgtcaac tgcattatcg acgatttcat agactaaatg atgcaagcct
    10441 cggctgtcag tggatccaat gtacatccct ggtctttttc taactgcctc taatccttcc
    10501 aatacttgga tggacgagtc attgtattcg ttgtttactt tttttgccaa ggcaaacgct
    10561 ccttcaataa tactttgcgg caaaaccgca ccacgattat catactagaa aaggtcgtga
    10621 aaaaaaagag ggtagacgag aaatcaattt taattatctc ctaaccgctt tttcgacatt
    10681 cccctctatc gatcatctct tctttgtggt catttttatt tttggttttc tttcaactta
    10741 ccaagttcaa tcttaataca acgatccatt acgatatctg tacgtcctgc ttttcttagc
    10801 atctcagctg cttctttgtt ttcaatccct aattgtgccc agaacacttt agcatcagtt
    10861 ttcaaaaaat cacctgctat ttcaggtaaa aattcactcc gtctaaatac atctacgata
    10921 tcgattggat catcgatttc tgtcaaattc gcatagactt tctcgcctag aatttcttca
    10981 ccagccaata atggatttac tggtatgatt ttatatccat tgtcttgcaa tacctgtgca
    11041 accatgtaac ttgttcgttc tggtttattg ctcaagccta cgacggcaat attttttgct
    11101 tcttccaaat agttgaaaat cgtttcttgt gttggatttt ttacagacat ttctgccacc
    11161 ccttgctttt ctttttattc catctattat acttgtatct ttcatttttt caattgttcc
    11221 tgttacctat tcatgctaaa ataaaggata gttttcaact tgaaagaggg taaaaaatga
    11281 aattgatctt attacttatt ttagcatatc ttttaggttc tattccttcc ggtgtctgga
    11341 ttggcaaatt gtttttcaaa aaagatatcc gacaacacgg gagcggtaat actggaacga
    11401 ccaatacatt tcgtgtatta ggcaaaacag ccgggattgt tgtcctgctt atggatattt
    11461 taaaaggtac attggctact tgtttgccga tgatcttcca cctttctggg attaaccctc
    11521 tttggtttgg ggtatgcgcc attcttggcc atacatttcc gatttttgct gggttcaaag
    11581 gaggaaaagc tgttgcgaca agtgcaggaa tgcttttagg atttaatcct gtattttttg
    11641 tttattcttg tatcatcttt atcgttagtt tattttgtac aagtatggtg agtttgagca
    11701 gtatgatcag tgcagtattg atcactttat ctacgatcgt ccttccttat gtagctcctg
    11761 tgattttggc taagcccaat tggttactga cgatcattgc ctttttagtt tcttccttta
    11821 tcttttatcg gcataaagac aatatcaagc gtatcagaaa ccatactgaa agtcgaatcc
    11881 cgtttggttt gaatagcagc aagaaaaaat cctgatactt tttcattcca aagagataaa
    11941 acgggagtgt gacaaatcga tgtcacactc ccttattact tacaggatag cttttttaca
    12001 caccttggta aacggtgttc cctgctaact tctcggtcac aacctcttac ttcactgtga
    12061 tggaatattt tgtcttgaag atttcatggg cacctagttg attgatccct actttttcca
    12121 tcaagtttcc actactgttc accgtgtcag cgattccgca ccacggttcg atacaaacga
    12181 aaggcgcttc ttttggataa ggagaccaga tccctacata aggcatatct ttatagctta
    12241 aagttacgct atgggaagat ttttcacttc gaatggcgta cgcatttaga ccccttgttt
    12301 caaaaaccaa ggcatcttgc tcaaaaagtt cacgattcaa agccagattc gtatttgtct
    12361 gacctaacgt cttttgatcc atatcgatga atgggccttt caatggtagg cgtaatcttg
    12421 atttcaatgg agaaaatgcc agatagaaat cttcaaatga caatccttct tcaagaggta
    12481 cattgaaagc aggatgtcca ccgatagaaa agaacatttc tttttcaccg gtatttgcca
    12541 cttgataatg aaccgtgatg ccgtctcctc ctaattcata gctgatcact aattcaaaat
    12601 caaatggata cttttctaat gtctccggcg tactccttaa gatgaaacta gccatttctt
    12661 gtccatgttc aatgacttca aaatccatat cccgagcaaa tccgtgttgc cccatttgat
    12721 atgttttccc ttgataaaca tactgatcat cttttaaacg tccaacaaaa gggaataaaa
    12781 ccggtgcatg tctgccccaa tacgctggat caccttgcca gatatactct aaaccatgtt
    12841 cttttgattt cagactgatc aattccgcac cgtgttcagc gatttcggcg atcagctggt
    12901 cattttgaat aataacagtc attgccattc ctccaacttc gacttacttt ttaaaatggg
    12961 aaggctggaa caaacatttc tatctgcccc agtcccctta cttctaataa ctgtgttttg
    13021 ctaactcgtc tttaaaacgg gaattcaata cttttaaata agttcccttc atgcctaacg
    13081 aacgagattc aatgattcca gctgattcca attttcgtaa agcattgacg atcacagaac
    13141 gagtgatgcc gatactatcg gcaatattag aagcagtcag acgaccttca tctccatcca
    13201 atgcttcaaa gatagcttgt acagccttca attcgctata agataatgtg ttgattgcca
    13261 tttggacagc cgttgcactc cgtacatctg cttcgatgct tcgagacttc tgataaagga
    13321 tctgcatacc gacaaccgta gctccatatt ctgctaagac tagatcgtca tctgcaaacg
    13381 attgttccat acgagataag ataatcgtac ccaaacgttc gccagctcca aagatcggaa
    13441 cgacagtcgt cagaccaaat ggatatttct ctcttgactc taccggaaat gcagtaatat
    13501 cactagtaat tgggatatta gcttcagttt tcatcaactg tgccactgta tcagtataag
    13561 attcaggaaa ttgtttctca acaaacattg ttttgattcg ttcattgttc acatcgtgac
    13621 gttcattata acctagtaat actccgtcac ttcctatgat ataggtgttg ctgtcaagaa
    13681 taccacctag tgtgacagcc attttatcgt aaggcaaatc tgcttgcatg tcaaaagtat
    13741 tcttttgctg caaaagtgtg tttatcttgc gggttttcgt tagcaagtct gtcattgtcg
    13801 tcctccgatt ttttataaaa tatagcgact taaatcttca tttgccgcaa ttgagccaag
    13861 tttgtcatcc acatatgctt ctgtgatcgt tatttctccc atttgcatat ctggtgcttc
    13921 aaataataga tcttctagta gtttttccaa aatggtatgc agtctgcgtg cgccgatatt
    13981 atctgtgtca cggttgacat cgaaagcgat ttgagcgatc cgttcaattg cttcttttgt
    14041 aaacgtaact gacacatttt ctgtaccgat caacgcgacg tattgtttta ctaaagcatt
    14101 atttggttcg gtcagaatct tcacaaaatc ttcagctgtc aaatcatcta attctacccg
    14161 tatcgggaag cgtccttgaa gttctggaat tagatcactt ggtttagaca aatggaaagc
    14221 tcctgaagca atgaatagga cgtgatctgt ttggatgatc ccgtattttg tgtttacttg
    14281 tgaaccttcg acaattggca atatgtctct ttggacgcct tctctggata cttctccaga
    14341 ttgctgtgat ttagaagtga tcttatcgat ttcatcgata aaaataattc cactttcttc
    14401 cgctaatttg attgcctcac tatggatatc agcatccttg actaattttg ctgattcttc
    14461 cttgatcaat aactctaaag cttctttgac tgttaccgtc cgttccactt ttttcttcgg
    14521 tgtcaaagca cctagagttt catttaggtc gatccccatt tgctccaatc cattgttcat
    14581 cattggtgca ggtttctttt ggtcttcaac ttcaatggtt acttcacgac tatccagtaa
    14641 acccttttgt agttgttcca agactgtacg gcgattgatt cgaatatctt ctgtcacttc
    14701 ttcttgcggt tcagcggtat tttgctgact agcattaaaa agattcatca tttgttcaaa
    14761 ttgattagtg gattgtttct gttctttttt gataccaggt accaagattt ttaccaaacg
    14821 tttttctgca ttttttttag cacttgtgta tacacggctg tactgctctt tttctacgat
    14881 ttggatcgca ttttctacta aatcccgaac cattgattct acatctcttc cgacataacc
    14941 gacttcagta aattttgttg cttcgacttt gacaaatggt gcattgacga tttttgccaa
    15001 acgtcgtgcg atttctgttt taccgacccc tgtaggtccg atcatcagca tattttttgg
    15061 tgtaatatct tgttgcattt tttcatctaa ctgcaaacgg cgatagcggt tacgcaaagc
    15121 aacggcaaca gatttttttg ctgcgtgctg tcctacgata tattcatcta attcacttac
    15181 gatttgtctt ggtgtttgtt ttaattcata catatctact cacctcttac agttcttcta
    15241 cgataatatt atgattagta aagatacaga tatctgcggc aatatttaac gcattttctg
    15301 caatttcttt cgcactcatg tcgttttgag catagttttt cattgctcgg gctgcagcca
    15361 acgcaaaatt ccctcctgat ccgatagcta agataccatc atctggtgtg ataacttctc
    15421 ctgtacctga aacaagaagc atttctttat cattcattac gatcagcatc gcttcaagtt
    15481 tttgcataga ttgctgtgtc cgccattctt gagctaattc gacagctgcg cgttgtagat
    15541 tcccgttata ttcgtttagt ttgccttcaa atttttcttc taaagtaaac gcatctgcaa
    15601 cacttccggc gaaacctaca acaacttctc cattgtagat acgtcgtact ttctttgcag
    15661 tgcctttcat gacaacagat tcacccattg ttacttggcc atctccagcc atagcaaatt
    15721 tcccgtcttt ttcgacggca cagatagtgg ttgaatgaaa tggtgattcg accatgtttg
    15781 tttcctccta aatttgttct tttccttatt tttacgctct tggatggaaa gaacgatagt
    15841 ttttttgtaa actttctttt gttacatggg catagatctg ggtagtcgat aaattggcat
    15901 gtcctagcaa ttcttggact gtccgcaaat ctgccccatt atttaataaa tgagtagcaa
    15961 atgtatggcg cagcatgtga ggatggatat cactatccaa actgcttttt ttgatcattt
    16021 gattcaagac atattcaatt ccagtaggcg tgattggttt tccatgatga ttcacaaaaa
    16081 cgatatcatg ctcttcgtga tattttgtca tcaatacctg gcggccttcc ctgaaatatt
    16141 cttgcagagc atcttgagca aaagaaccta gaggagcata tctctctttg ttccctttac
    16201 catggatcaa catgacctct gaatcaaaat caatgtctga taccgacaaa ctgctacact
    16261 cacttaaacg gatgcctgac ccgtaaagga cttccagtaa agcgcggttc ctcaaatcca
    16321 aaggactatc tcctgctacg ctgtcaaaaa gtgcttgcat ttcattttca taaaaaaaac
    16381 gtggcagtct caaatttttc tttttgagat gaacgtaaga aaatggattt tcttttatca
    16441 cttcttcttt gagaagatac tgataaaacg aacgtaggct ggctatcttg cgactgatag
    16501 aatttctgct atagctagga cgatgatcag ctaatgcact caaatagaca cgaaccgtaa
    16561 aatattccgc cttcagcaga tcagcgtctc cagattcctc caaaaaatgt ttgaaatgga
    16621 gaatgtcttc ttcatatgct tctttcgttt tttctgaata tcctctttcg acaatcaaat
    16681 aacgcaaaaa tttttgttcc cacgtggctt ctgtcatttt ctcaccttcc ttttgtccca
    16741 gagaataatt tagcacagcg tttataaaaa gacaaataaa ttgtcagaat ttaaccaata
    16801 tttctaagtt tgttcacaat tggcgcaatt cctcacattt tgtttgagaa attcttaaga
    16861 aaaacgcaag aaaaagagcg agactatccg tctcaccctc ttcatggagt ttccttactc
    16921 atttgggatt gcagtttttt ctaattctgt ttttattgcc tcattagctt ttaaagctcg
    16981 ttgtgcaatt gcttcatatc ttgctttttt gtctcgaatg cgctctggca gttcaggaaa
    17041 cagaccatag ttggcattca tcggttggaa atgttttcct tccgcatgag taatgtagta
    17101 agccatactt ccaatagctg tttcttgtgg aaactcgact ggttccattt cttttgctaa
    17161 gcgtgcggcg ttgattccag caagtagtcc gctcgctgca ctttcaacat agccttctac
    17221 accagtcatc tgacctgcaa agaacagatc ttcgcgtttc gttgattggt aagtcggttt
    17281 cagcaattct ggcgaattca taaagctgtt tcggtgcatc acaccatagc gaacgaattc
    17341 agcattttct aacccaggaa tcatccggaa aacacgtttt tgctcgcccc atttcaagtg
    17401 agtctgaaag cccacgatgt tgtataaaga agctgcagca ttgtcttgac gcagctggat
    17461 aacagcataa ggacgtttgc cagttttagg atcttccaat ccaactggtt tcataggtcc
    17521 gaaaagcatc gttttgaatc ctcgctgtgc cattacttca attggcatac atccttcaaa
    17581 atatttttct ttctcaaaag atttaagttc tgccacttcg gcattgatta gtgcctcatg
    17641 gaaagctttg aattcctctt ctgtcatcgg acagttcaag taagctgctt ctcctttgtc
    17701 atagcgggac ttcaaatata ctttatccat atcgatcgta gctttgtcta cgattggtgc
    17761 agctgcatca tagaaataaa aaccatctga accattgaat gctttgatgc tttctgctaa
    17821 tggatgagaa gtcaaaggtc ctgttgctac tacggtgatt ccttccggta tttcagtaat
    17881 ttcttcgttt atcactgtta ctaatggatg ctctttgact tttcgggtaa ttgtttcaga
    17941 aaaagaatcg cggtcaacag ctaaggcacc accagctggc actgctgttt catctgccga
    18001 agtgattact accgaattca atcggcgcat ttcttctttc aataacccta ctgcattagc
    18061 caaactgttt cctcttaatg aattggaaca tactagttca gcaaaattac ttgtgtgatg
    18121 cgcctcagtc gatttgactg gacgcatttc atataaacgg acagggactc ctgcttgtgc
    18181 gatctgccaa gcagcttcac ttccggcaag ccctgctcca acaacattta ccattttcaa
    18241 tgaaagaacc tcttttctat tatttttgga tgtcttcttc gtaatctcca tttacacaga
    18301 ctacttgttt accgcctttg actttcttct cgaccagata tccgtcacat tttggacaat
    18361 ttctgcccac tggtttatcc caagaagtga attcacattc tgggaaacgg gaacaaccat
    18421 agaatagtct gtttttctta gattttcgtt caatgacttg tccttcatga catactgggc
    18481 aagtcacacc gatttctttt acgattggtt tcgtattccg acaatcagga aaattactgc
    18541 aagcataaaa cttcccatat ttccctaatt ttatcaccat cggatgtcca cagacatcac
    18601 aatcgaatcc tgctggttca tctttgattt gaattttttc gattttttct tccgcattcg
    18661 tcagttcttt ttcaaacggt ttatagaaac gatcaaccac tttgacccac gcttccgtgc
    18721 cttcttcaat tttatccaaa tctccttcca tttctgctgt gaaatggata tcgacgattt
    18781 gagggaagaa ttcacagatt aatgaattga caatttctcc tagttcagtc ggctcaaatc
    18841 ttttttgtgt caattttaca tagtagcgtc tttggatcgt atcaagtgtt ggtgcataag
    18901 tggaaggtcg ccctaccccg ttttcttcta gtgcacgaat caacgtagcc tcactaaaac
    18961 gtgctggtgg ttgagtaaaa tgttgttttg gttcaatatc aacagattgg actacatcgc
    19021 cttcttcaag ttctggaaga atattttctt tctcttcttt gccgtcatct ctaccttcca
    19081 catacacttg catgaatcct ttgaatttga tttttgaacc gttcgcaatg aagatgacac
    19141 cattttgttc taatgtcact ttcattgtat ctaaaatagc tggtgtcatc tgactagcca
    19201 caaaacgtga ccagatcaat gtataaagtt tcaattgatc tttatctaaa tattgtttga
    19261 gttcatctgg tgtacgaagc acactcgttg gacgcacagc ttcatgggca tcttgtgcgc
    19321 cttgagcatt tttgatcttt cggtgaccat gtctagaaaa ttcttttcca tacgtatctt
    19381 caataaatgc tgcggcctct gcttttgctg tatcggcaat ccgggtagaa tcagtacgca
    19441 tgtatgtaat caagccgact gttccttgtt tacccaaagt gattccttca tataattgct
    19501 gtgctaccat catggttttt cttgttcgaa agtttaactt acgggcagct tcttgttgca
    19561 ggctacttgt agtgaaagga ttcgcagggt ttcttttgcg ctctttcttt tctacttttt
    19621 taacttcgaa gtctgttcca tcaattctag cagtgacttc ttttacactt tgtgcatctg
    19681 gtaatttctt tttcttaccg tcgattcccc agaaatttgc tttgaatttc ttgcgagctt
    19741 ttttaaaatt accgtcgatg ctccaatatt cttctggaac aaattctcgg atctcttttt
    19801 cacggtcaat aataattttt aaagcgacag attggacacg tcctgcactt aaccctttct
    19861 tgaccttacg ccagagaata ggactgatcg aataaccaac gattctgtct aaggtacgac
    19921 gagcttgctg tgcatctact aaatctacat cgatcgttct tggttcctta aaagctgctt
    19981 tgactgcgtc ttttgtgatt tcattgaaga ccacccgatt tttttctttc agatccaacc
    20041 caagtaggta cgctaaatgc caagcaatcg cttctccttc tctatccggg tcacttgcca
    20101 agtaaacttt ttgagctttt tttgctgcgg ctttcaggct tttgatgaca tcgccttttc
    20161 cgcgtataga aatataatgg ggttcataat tgttttcaaa atcgataccc attttacttt
    20221 tcggtaaatc tcttatatgg ccaacactgg ccattacttt atagtttcgt ccaagatatt
    20281 tctcaattgt cttggctttt gcaggtgatt ctacaatcac taaatattta tacgccatta
    20341 ctgaatggac tcctttccat tgtcgtgttc ctactattaa aagaggattc atacaaatcg
    20401 tagctaatca tatccattca gaaaacgttt gtcaagaaaa gcaaatgaaa agtatggtgt
    20461 tattctttaa attctgccaa gatatcttca cccgaaaccg tgcaaactgc accgtcttgg
    20521 atcaaatgat gacatccggt agaatgttct gttaaaatat ttcctggaac agaaaaaact
    20581 gttcttccaa gatccaatgc ttgctgtgct gttatcagag agccgctttt tttcttggct
    20641 tcaaagacac atgttccttt agacaaagct gcgataatcc gatttctcat tggaaaacaa
    20701 tgctttttcg ggccggattg aggaggatat tcactcaaaa gaagatgatc atttgcaatc
    20761 acctcttgca agtcacgatt ttcagcagga tagacttgct ctatccctgt tccaatgact
    20821 gcgattgtcc gtccaccatg agagattgct gcttggtgac cgtatgtatc tatccctttc
    20881 gcgcagccac tgacgatcac atatccagct tctatcactg gttttagtaa ataataaact
    20941 gtttttttgg ctaaagcaga agtgtgtctt ccaccaacaa cgcttaacat gttacaagaa
    21001 agtaaagata gatcaccatt ataaaacagt acaattggcg gataggtcaa gtgaagcagc
    21061 tgagggggat atttgggatc aaagcaagtg ataaacgact gtttcgattg gattttgccc
    21121 cagttagcgt gaatttcctg ccaacttttg cgaaacagct cccggtgttt ggttatttta
    21181 cctatcttaa tgatgtcttc aaatccgatt tctttataag gatactgcat catccagtta
    21241 gcaagtgccc attttccttg actactgatt ccttttgtat aagcaagttt caataaattt
    21301 tcttctattt gatacatcat aaaaccgcct ttcgtctctc tttctaataa gatacgtaaa
    21361 agcggctgtt ttttattgta aatatttttt tacaggtgca aaacttttac gatggattgg
    21421 cgtaattcct tgtcgttcaa gtccttctaa gtgttccttt gttccataac ctgcattatg
    21481 cgagaaacca tatccaggat aaagcacgtc atattctttc atcaattggt ctcgatatac
    21541 tttagcaatg atacttgcag cagcaatcga cacagatcgt gcatcccctt tgattatttt
    21601 ttcctgctga ataggcaaat ccaaggtcat cgcatcgatc aacaaatagc ttggtacaat
    21661 ggacaatttt tctactgctt ggatcatcgc taatttactt gcttcataaa tattcacatc
    21721 atcgattgtc tgctgatcaa cgatcccgat cccgattgcc agggcttttt cttgaacttg
    21781 ttgataaaga agttctcgct ttttctctga taattgtttg gagtcattca gccctaatat
    21841 gatctcattt ttcggcaaga taactgcagc tgccacgaca ggtccagcta atggtcctct
    21901 tcctacttca tcaatgccgg caatccattg gtgaccttgt gcgtaccctt gcttttcaaa
    21961 gcaaaacatt tcttcatatt tttccttaat taatgcttcc tttttcaatc gtcgttcaaa
    22021 agaagccagt gcttgctgca cgcctttgcg tggatcattt cttaatgatt ctagtgtagg
    22081 atcatctatt ctctgtatgc ttttcaatcg ttctttgata ctttggatgg attcagtcat
    22141 cacttttttg ctccatttct tcgcaacgat ccagtgtgta tcgtccaagt tttccttgac
    22201 gaatatcatg cacgactaat tcgctgccac gatcatagtc ttctttatac ccacgttttg
    22261 ctgtaatatc catcagtaat tccggtgcca atttttcttc ttgctcgatt gataaaccat
    22321 aacgatcgct gattcttcct gaataatatc gggcaaaaaa gtctaatccg taaatcgcta
    22381 aatcatctaa atggacaagc tggtctttga tcgcgcctgt cagcgctagt tttttgccga
    22441 tttcttcatc ttcaaatttg ggccataaaa tccctggtgt gtctaacaat tccaactcat
    22501 tacctaaacg aagccactgc tgaccttttg ttacgccagg cttatttcct gtctgtgcaa
    22561 ttttcttgcc tactagtcga ttcatcaacg tagatttccc aacatttgga atacctaaga
    22621 ccattgcccg aatagcgcga ggcttgatcc cccgagcagc atctcttgcc cgtttttctt
    22681 tcaatgcttc tttagccgca ggtactagac ctttaattcc tttactttct tgtgccgtga
    22741 tagctaatgc aagatggcct tgtttacgaa aatattcttg ccacagcttt gtctgttgtg
    22801 gatctgccag atccgcttta ttcaataaaa caaggcgagg tttttgctga atgatctgat
    22861 ctagcattgg attacgagaa gataaaggta atcgtgcgtc taccaattca aatacgatgt
    22921 ccacatattt cattttttca gaaacttctc gtcgggcttt tgccatatgt cccggaaacc
    22981 attggatggt cattgttctc tccattcctt tcataccgga atattccccg gctttacttg
    23041 tttttttctt tttcaatata aattcacttt tttattatct taattgtcaa attaagttag
    23101 acaaatcatc aatacttacg taactcaaaa atacttccaa aggtgttcga taattcagtg
    23161 attttctagg aatattattt cgtttcgatg cgacagattg aataaaagat tcatctactg
    23221 aattgaaatc catagatttc aataaaccat ctctacgtaa caatccgtta gaattctcgt
    23281 ttaggcctct ttgagacggc gttcctggat cagcgaaata aatggcaata tcattggcat
    23341 tactgatctg tttccaattt gaaaattcct ttccacaatc aaaagtgatg gatttgaata
    23401 gatttttcgg tacagattca aaccaatgat ttaatttgtt ttcaatatca atcgcttgtc
    23461 taccacacgg tttcaatgtg atgataactt ttgataatcg ctcaactaag gtaattacag
    23521 cacttttatg tttcaaacct acgatagtgt caccttcaag gtgaccaaac tcatttgaga
    23581 attggctata atctttttca cgttcatgaa tagagcgacg gaaagcttgt tttccacgtc
    23641 tttcttgatg tccatttggt ttacgcttgc ctttcatcgg taagtcagaa ggattaaata
    23701 gccccttttt gaacatacga taaatggtac gagcagaaca acgaatagga aacgctgcac
    23761 gaccaacaat cacatcgggt gtccatcctt gaacaacctt tctttgaata tattctgatt
    23821 gttcctcagg taaaacaagc gggcgtctac cacagtttga tttgtttttc ttatactgtt
    23881 gatagtactc taaggctgat tttccttgct tgaagaacag gtatacttta tggatcgttt
    23941 gtcttgaacg attcaagaaa tgcgcagttt tcgcaacaga ttgatttatt ttgaaataag
    24001 actctatcat tacaagttca tccgttgtaa gatgggtata ggtcatttgt actcactcct
    24061 tataattctt tggacggaac tattttgagt gtagcacaaa tgactttttt agttgtctag
    24121 cttaatttta caatcggcgt tataaagaaa ttccatctat tagttaagtg aaagtgaacc
    24181 agtcctcatt ctatcacatc ttcacttttt tgatgatgca aatttttatt ttggtttcta
    24241 tatatgaatc taaatttttg acagcgtttc ctaaataaga gatgatagac gaacaaaaca
    24301 gaaaggagac gaaacaaatg aatagtatgg tttttgttaa ttttcctgtt gctgatgtca
    24361 atcgttccgt tgctttttat gaaaagctgg gttttaaaaa aaatgaagaa ttttctactg
    24421 aagaagctgg ttcaatggta tgggatgaca ctttttggat aatgcttctc tctcatgatt
    24481 tttataaaaa atttataaaa gataaagaaa tagctgataa taaaaaaaca agtggtgctt
    24541 tgatttcgtt tagtgttgaa aatgcggatg aagtaaaaaa atttgctcaa attgcaaaag
    24601 aaaatggtgg tactttccac gaagtagata tgggaatgcc tgaagaagaa atgttttcat
    24661 tagaagtcca agatctagac ggaaatacat tagaacccgt atggatgaag atgtaagcag
    24721 ataactaatc tgacgttttt tataacaaaa aaacaaaggc ggatgatacg ataagtattc
    24781 tccgcctcta ttttttttat tcgtacaatc tcttttttac agtttataaa agcagtcttc
    24841 tcaccctttt tttaattgtt agatgccagt gtatgtaaat caccaatgtg tcatgacaca
    24901 ttggtgattt tatggtatcc ttttattatt aactgacaaa ggagagaata ttttgaacac
    24961 tttgattgca aaagacgttt ttagattgcc cgaaatgaat gcaagattgt tgtcgacctc
    25021 tgctctttta ttagcccttg atgtcatctt atacaagata gcgattggtc ctgcttattt
    25081 tcaaatcaca ttcggatttt taagtatggc gcttatgggt tatctatatg gaccgatctg
    25141 ggctggtttg cttgaaatgt tgtcgaacac tttaaatttc acgatatttg gttcaggaac
    25201 gtttcaagct gcttttctta tcacggcttt ccttggtgga tttattaatg gcttattttt
    25261 atatcataaa aagataaacc tgccttccgt catcgctgcc caattattga ttatgatccc
    25321 agtcagcctt tttatcaatt cttggctgat gtctgttttg tttggtgcag atttcaaagt
    25381 aatttttcta ggtagattca tcaggaatat gatccaaatc cctattcaaa taatggttgt
    25441 ttattcattt atcaaaactt tagaggctag aaaagtgttt aaacgagctg tttgatttta
    25501 cgtactaagg tagtaaactg attttggatt aaacaataaa gcgtgcagtc tcaacagact
    25561 gcacgcttta ttgtttctca ctggatccat tttatttcat caagtggata atagacaaat
    25621 cttgctttac ctagaatatc agttttattg atcgtgccaa acgaacgact atcttttgaa
    25681 actcttcgat tatctccaag tacaaaataa ttatcttccg gaagcttgga ttgtcccgtt
    25741 agctcttgta gtgtaaagtc cgtcgtatag gatgcatttg catgatctga tttcaggttt
    25801 ttagttagat aaggttcctt aactactttc ccattgatct tcaattgatc attttggtag
    25861 ctgacatttt ctcctggaag accgattact cgtttgatat aaatcgtgcc atctgctaat
    25921 tgaaaaacaa caatatcaaa tcgtctgatc tccgaaaatt tttccatcac aaccatatca
    25981 ccttgcttca acacgttttc cattgaattg ccttccactg gtaccgggat gaataaaaat
    26041 ccccgcaaca tgaacgcaag gaatacagaa acaagtaagt atttgaatac aagccagaag
    26101 cgattaatat agcgttgttt ttttgtcaat ctctatcaat ccatccctct ttttttcatt
    26161 ctttctttat tataagaaaa atatcaaaaa aatgcgacta gatagctgaa ataaaaataa
    26221 ttctttcaat taggctattg gacaaaaaac ttttttgatc ataaagtaca aaactatctc
    26281 ttatatgtca tgattatttt ctaaatattt ttcccatgct tcgtcaaaga tatccatgga
    26341 cggcaaatag tcgacattca gttccagata attagaaatt tcttcgtagc tagagctgtg
    26401 cttaggaaat tggatgtctt taccggcttc tgtagcgaaa acagataaag catcatgtgt
    26461 gccaccgcct cgcagtgtca ttaaataatg aaaaaagctt cgattcaccc ttcgttcctc
    26521 caattttctt cgataaaggc agcccggcgt gcatggtttc ttttaaaatt ttcaggatgc
    26581 tttttataat actcttgatg gtcttcttct gctagataaa atggttgggc tggttcgatt
    26641 tttgtaacga ttggctgttc gaaacgtcca ctttcagcta actgtttctt acttttttca
    26701 gcaatgatcc gctgttcttc tgtcaaataa aagatcaccg ggcgataatt gtcaccgcga
    26761 tcctcgaatt gtccgaaagc gtcagttgga tcagtttgct gccagtaaag ttctactaat
    26821 tgttcatatg aaatcaatgt tggatcatag ataatctcca ctgcttctgt atgtccagtc
    26881 gtttggcttt ttacttgttc ataagtcgga tctggtacat gccctccagt atatcctgac
    26941 atcactgtat ggataccagg aagcgtgtcg aaaggctgga tcatacacca gaagcagcct
    27001 cctgcaaata ttgctcgttc cattatgatt ccccctctaa tactactcat ccgatgattt
    27061 tttattttct tttgtcttgc tttcagtact gctggtgcta gttttgttgg tatttgagtt
    27121 gtttgaagca tcagaggtat cttttggcaa atatagacta aaccgaatgt catcatttac
    27181 taagtcgatt ttatctgctt taatatacat cccgttttgc attcggaatt gatccaacct
    27241 aagcatgata gtcagatcat ctgtattgac ttcgatccaa tctggtaatt tatagtttct
    27301 cttgatcata ttcatcacat ctttgattgg caaactcaat gttccgatag aaagacttct
    27361 agcttttagt tggacatttc cgttttccat cacatacgga tcaaaatata aatagaaatc
    27421 gataggaaaa cctagtactt cgaaagtacc attgagtagt gcttcatttt ccaaataaaa
    27481 tttatattca atatcactat ctttttgata ctcactaagg aaaaagctga tgatttgatt
    27541 gactttttct ttatttgaat tgatcgttaa gacaggatct ccttgtcttt ccgtaagagc
    27601 tggtatttct gggagttctg gttcccgatt agcaaaaata cggctgccta aaaatacggt
    27661 cgaaccaagt actaccccaa gaaggataag gaaggcgaat ttccagtaat ttgttttctt
    27721 tgttttgttt gaagatacgt cagatcttgt tttcatgttt attgatcatc ctccttctct
    27781 aaccattggt tttgtgtctc gatcagctga tctctcactg catttgccat caattgataa
    27841 cctaaattat ttggatggaa atgatcttct tcatagagca cattattttt gatgccattt
    27901 agattttctg tcgtgctagt tgtttgatct gttccttctt ctgtgacacc tacttcctga
    27961 ttcaatcctt gataaagcaa atcattgatt gggataaagt agcagttcgg catttttttt
    28021 gtcaccgttt ctgttccttt gttccaattg ttaacaattg tctgcatgtc agtgatctca
    28081 ggaaagttca aataaaatgg attgtagatg cctaaaatgt aaataggtgc ggttttattg
    28141 agggatcgaa attcttccaa tagttctgtg acattttcct gatagttttt cagtggcttt
    28201 ttaaaggaat tgaccgttaa cccgaataaa ttattttgga taactttcat caaatcatta
    28261 ccgccaacag tcagtgtgat aaaatcagct gttttgatat tgtttcgaat ttcttctttt
    28321 gtttttactc gctttaagat ctggtcactt cgttcaccat ttacaccata gttttctgtt
    28381 tcaaccgcgg tcaaattata acggtctttt agatcatttg cgacaagggg aacgaatcct
    28441 ccttgcttgg tttgatctcc tactccttca gtcagcgaat ctccgatagc tatgtagtga
    28501 agtttttctt ttttatttgg atcttctctc acagtagtta ctggtttcat tagtgggcca
    28561 gctttcggta tagaagcagc taaacctgca tagatgatga tcgcaattaa taatgggaac
    28621 aaaacgagga tcaaatgtcg tttcgttaat ttcatttttt cacctcttgt attcgattgg
    28681 ttctaatgtt atcaaaaatt ttatcaaaaa ggtagtcttt atgaaaaaag aaaacaatta
    28741 ttttcctaat tttataaaga aaaagagaat aagctgcaag cctttcaggc tgagggctta
    28801 tcctcttttc gactaatcta cataatacat gattgcaaac gcgtctttac cagtatgcgt
    28861 agcgataacc ggattcgtat gcagtacagg gatatgcatg tccgggaata attcttgcaa
    28921 accctctttg aattcttgcg ccaattcttt tgcatccgca tgtgaaatac cgatttgtcg
    28981 cacatttggc aattctttca gttcattttt caaattatta aaccatttat cgaacgtttt
    29041 ctttcctcgc cccttagtta cagggattaa ttcagaatgg gcgaaattca tcaccacgcg
    29101 tatgttcaaa acactagaca gcaaaccagt tgttcggctg attcttccac cttttaccaa
    29161 gttatctagt gtggatattc cgatgtataa ttttgtattg tcttgtactt ttttgacttc
    29221 ttcaaggatt ttctctactg ttttaccttc ttttgctaat tcagctgctc gaattacctg
    29281 aaaagataat ccttggtcag tggtatcact gtcgataacc gttactttcg tttttgttag
    29341 attactggct tgtcttgctg cttctactgt tccactcaag ccttttgtca tatggatcga
    29401 aatcacttca ctgccgtctt ctcctagctc atcatacatc gagatgaatt ctccaattgg
    29461 tggttggctt gttttgggca gattttttgc tttcgccatc atttccataa atctttcgcc
    29521 gtctagttgg tcatcatcgg gatatactgt tccgtcgatc atgatggata gcggcatgac
    29581 acgaatgcct aaagcatcgc gtacattttt ttccatcgta catgaagaat ctgttacgat
    29641 ttttacattt gtcattattt tctctctctc tttctttaac aagatgcctt attcatgtta
    29701 aactacatgt aagacaattt ctatttttag tataacagac aaataatgat ttttcattaa
    29761 ttacacctag taaaggagcc gttttcatgg aaaaaacgaa atttaatcga acgtatgaaa
    29821 tattaaatga agtgtttaat gcagtcaccc atggaattgg gactggattg agtatcgcgg
    29881 gactagtggt tcttttagta aaaggtgccc acttgcattc ctccctccat attgtctctt
    29941 atgctatcta tggatcgatg atgattcttt tatttttaac gtctactttg tttcattcat
    30001 tgatttttac aaaagcaaaa aaggtgttcc aagtattcga tcatagttcg atttttcttt
    30061 tgattgcagg aagttatact cctttttgtt tgctgagtat ccgaggatgg ctaggttgga
    30121 ctttatttgt attaatctgg ttgtttgcga ttcttggcat tatatataaa gcgttgacgt
    30181 tgcataaacg ggagagagta tcaaaagtat ctacgatcat ctatattttt atggggtggc
    30241 tctgtgtcgt ggctgctgtt cctctttatc actctcttgg aatgactggt gttattttga
    30301 tggtagcagg cgggctttct tacacgatag gcgcattctt ttatagcttg aattctatcc
    30361 gctatatgca tgttgtttgg catttgtttg ttatgttagg tgcaggtttt atgtacttct
    30421 ctgttttatt ctttacttaa taaaaaaaat aaagctatgg atgggacagc tctaaataac
    30481 agctagtcta tccatagctt ttttatatcg tcaattcgcc tgtcccgctt tttacaggat
    30541 tctcattacg ctgatacgtt tcaaattgat gcggataacg atttttttca tccatttctc
    30601 cttgactagt attgatcagt gaccaatcag accagtcaac aggtggaaaa taagcatctc
    30661 catcgaatac atgatggatg actgtacggt ataatatcgt acaaaatggc agaaattctt
    30721 gataaacagc tgaccctcca gcgatgaaaa agataccgtc tgtttctttt tccagagcaa
    30781 gcacgtcttc cacactatgc atgacatgca cgccttctgc tttataggaa gaatctctcg
    30841 tcaaaacaat cgtctgacga ttcggcaacg gacggctacc catcccttcg aatgtttttc
    30901 gtcccatcac tagtgtattt gcttctgtca tttgtttgaa gaatttcaga tcatttggca
    30961 aacgccaagg aagacggtct tcttttccaa tcaatccgtt ttcatcctgt gcccaaattg
    31021 cagctaacat tttctcatcc tttctgattc taaacagcga taggagcttt gatcgccgga
    31081 tgtgggtcat atccttctaa ttggatatct tccatctcaa aatcaaatac tgactgcttt
    31141 tcctgattca aaactaaagt cggaaatgcg cgtatttctc ggctcaattg ttctttcatt
    31201 tgttcgatat gattgatata aagatgagca tctcctaatg tgtgaacaaa ttcgccaacc
    31261 gctaaacctg tttcatgtgc aattaggtga gtcagcaacg cataacttgc aatgttgaac
    31321 ggaacgccta gaaacacatc cccactgcgt tgatacaatt ggcagcttag ccgtccctct
    31381 tgaacataaa attgaaacat cgtatgacaa ggaggcaaag ccatgctagg aacatcttcc
    31441 ggattccatg cagaaacgat caatcttcta gaatcaggag tctttttgat tgcttcgatc
    31501 acttgtttga tttgatcgat aaagccaccg tcttttgttt cccagtgacg ccattgatag
    31561 ccgtagatgt gtccaagatc gccgtatgtt tcagcaaagg aatcatcggt taggatcttc
    31621 tcgcaaaaaa gcttgctctg ctttttatat tcttcattga attcaggatc tgtcaaacat
    31681 ctatggccaa aatcagtcat atctggtcca ttatagtctg gacttttgat gtagcgttca
    31741 aatgcccatt catcccaaat atggttttta ttttgtaata gatagcggat attcgtatct
    31801 ccttttaaaa accaaagcaa ttcgcttttg atcaatccaa aaggtactcg tttcgttgtt
    31861 aacaaaggaa accctttttg cagatcaaac cgcatttggt atccgaataa gcttaatgtg
    31921 cctgtgcctg ttctgtcttc tttacgatgc ccttcttcca acaattttct tcctaagtca
    31981 agatacgctt gttccatcct ttttcctcct aatcttctac ccattcactc aagtattccc
    32041 accgtgccat tttttcttca agttgtgctt ctgtttcaga tacgtcgttt tgcaattctt
    32101 gtaatcgagt gaaattatct ccttgatgat tcatctcttc ttggagcaat gagatttttt
    32161 cttcaagttc tgcaatctca tcttcaatcg tttcccattc tttttgttcc atgtaagata
    32221 atttcttctt ttcagcaggt tccttgctct gtatttttgg tttcgctttt tcagcaggct
    32281 gctgggtctc tttttgcttc agtaaataac tggacatgtt tccgtaataa agttcttgtt
    32341 ttccgtttcc ttggaatact aataacttat ctgctacctt gtccaagaaa taacggtcat
    32401 gggaaacagt gatcacagca cctggaaaac ttctaaaata atcttccaaa atcgtcaatg
    32461 ttgctatgtc caaatcattg gtcggttcat ccaataataa aacattaggt tgctgaatca
    32521 gcagtttcaa caaaaataac cggcgttttt ctccgcctga aagtttgcct atcagtgtcc
    32581 catgcataaa tcttggaaat aaaaatcgtt caagtaattc tgctactccg atttgactgc
    32641 catctgctgt ttttgcttct tcagcagctt cttgtaagta agcaatcatc cgtttatctg
    32701 gtgccatttc ttcattttct tgtgtataat acgcaagatg aaccgtttca ccgatagaca
    32761 ttgtaccgct atccagggga attctgccag ctagaatatt aagcaatgtc gattttcccg
    32821 caccattttt accagtaata cctagtcttt cccgagattg aatcaataaa tctaaatgtt
    32881 ctagcagtgt ttggtcattt atacggtaac tgccatcttt gatttccagt acttttttcc
    32941 ctaaacgttg tgtcgctagg ttcagctcta aatcatcttc ttggtttact tggtacaagt
    33001 tctcctttag atcttcgaaa cggttgatcc gtgcttgctg cttggtgcca cgtgcctgta
    33061 cgccagccct catccaagct aattcctgtt tataaagccg cttgcgcttt tcttcttgtt
    33121 ctacttctac acgatcacgt tctgcttttt ctaagacgta agcttcataa tttcctttat
    33181 actcgtataa ttttccaaat gaaagttcga agattcggtt tgctactcta tctagaaaat
    33241 aacgatcgtg ggtaaccatc aatagtgcgc cacggtattg tttcaaatag ttttccaacc
    33301 actcgatcgc ttcataatct aaatggtttg ttggttcgtc tagtaatagt aaatcgggtt
    33361 catcaatcaa cacttgcgcc agactaactc gttttttctg accacctgat aaagtactga
    33421 tttttttatc taattcactg attcctaatt tttgcaaaat aatctttgcg ttggtatctg
    33481 tagtccaagc atcttcttta ttcatttgtt cttctgcttt agcataacgt ttttgcacat
    33541 cagggtcttc tccgttttcc cctagttcga tcaatgcttc ctcatatgct cgaacagttt
    33601 ggatcaaggg gctgtttcct tgaaagactg cttggagaac agttaattca tcggaaaatt
    33661 cagctgcttg agaaagataa ccaatacggt aatcagttgg ataaaacact gtttgccgat
    33721 caccgtcgct gctatcgata ccggcaataa tatttaagag acttgtttta cccgtcccgt
    33781 tcgtcccaat caacccgata cggtcttttt cgtggaccaa aaaagaaatc tggtcaaaca
    33841 aatctttttc accataagtt tttttgaggt cagttacttt taattctttc aacatgttcc
    33901 ctccttatct tggatattgt atcataaaaa tagtgaactt caggaaaatt tttttggtat
    33961 ttgttctttt tgtgttctca aaaaaagaac aggaccgatt cgatcctgtt ctgatatgtt
    34021 ttgtttttta taaaacttga aaatttttac ctttttattt tattggactc gttttttttg
    34081 actttgctcg aaacgcaaat cggaaagaaa agttatcaga tggtttgttc atcgtataag
    34141 attctaacaa attgatattc ctgcgatagc tgttcgcaga aaacgtcgtg attttttcag
    34201 caacttcgta ttcatcaatg atttttcttt caagataata acctcttagc atttttttcg
    34261 taaacagact atcttgctgg atcatccacg aagaaatcaa attccccgag ttcattttta
    34321 ttccttcaag tttgcgttgc tggataaaaa attgaaccat cgtttcatca taaatatcag
    34381 tcaagttatc taaactttca ataatcaact gagtattttt ttgatatgtc tctctgattt
    34441 cttggatatc ttcatcacta ataaatgtct gacgatcagc atatcttctt tgccaaaaca
    34501 ttttcggatg gagaacgatt cgcattaacc tacggacaac aatgaaccaa aagccgataa
    34561 aagacaaaat ctggcttgtg atagaatgtt cgaatcgaga aagaaaacgg gagtaaaaac
    34621 gataaccatt agaagaaagc ttgcccgaac gataactttc atccaatcca tcccgttcga
    34681 tcgacaaaat cagtgcttgt atttcttgta cttcttgctt ttcatcatcg gttaaatctt
    34741 ccaagtacag atcacgtatc cgttcttgat aattttctat cactgcttca gtggctaaaa
    34801 attcttttgt attacgatca atttccttta tttcttttcg caaaacttca acgacctctt
    34861 ctaaaatcat caaagcattc aaatccgtgc ttttcgcttc ctctgtttcc gtcagcatag
    34921 gcaagacaat gatccctatg accaaagtca ctaagatgac acccgccgtc agaaacaaaa
    34981 gcaaagaccg ctggtaaaac atcatattat tgattgaaaa tggcaaaata aaaatcgtag
    35041 ccaaactgac tgttcccttt acaccgccaa atgttaagat caagatctcg ttcaactgtt
    35101 ttttgaactt tttactgcca tctttgaata cataaaaaag actgatagaa atgaatctga
    35161 tcacaaaaag catgacagag attaatacga taatcgctaa taaaagaccg tttggatata
    35221 aaccgtctcc ccatactggc gagaacactt gcgtcaattc gattcctaaa aatataaata
    35281 ctaatgcatt cagtgtaaaa gcaatagttg tccatgtact gtgggacaag ctcgacaatt
    35341 ctgcatcaaa cacagagatt ttcctaaaac cagaagcttg gagaatacct gctgccactg
    35401 cagcaatgat tccagataca tcaaatgcct ctgccaatac ataggctaga aaaggaagca
    35461 aaagttcgat cagcagatac cctgtgacat cttgtgctga tgctttttcg atcaaatgaa
    35521 ttattttttg tttgaaccag acaagcaaaa agccgatcaa agctccccca atactagaaa
    35581 ctactagact catccccgcg tttactgctg aaaaagatcc ggtgatcagc gcaccaagag
    35641 caaactggaa ggcagtaaca ccagatgcat cattcaaaag accttctcct tccaaaatat
    35701 gcatcgcctt tttagggata ttcactcgtc cagaaagaga actgactgct accgcatctg
    35761 tcggacctaa cgctgctcca aaagcaaagc aggcagcaag tgggacactt ggcaacagga
    35821 atgataacgt cgcacctacc ccgacaaggg tcaaaatgac tcctccaaat gccaagaaca
    35881 aaattgtccc gaaatttttg agaatcgaag aaatatctgc tttttctcct tctctaaata
    35941 acaatggtgc aataatcata actaagaaca attcaggttc aaaatcgatt gattgtcccc
    36001 actctgtcag acctaatacc actccaagga aaatttggat cagtggagct ggaatcattg
    36061 gcaaaatgcg gctagctatg ttcgataagg tgatcgttac tgcaaataca ataactaact
    36121 cagcaaattc catcgtccat cccctttatt ttttattttg tgtaagcatt tttaaaaatt
    36181 gttctttttt ctttaaaatt ttggtttcga ctacttcaac ctgtgtttgg ttcccctcta
    36241 attcacactg tacacgtttt gactccaaac gaagcagatc atgtttcaga cgttccaact
    36301 gacgatacat caatatattt cttttacgca acttacaaag tatttgacgg cggtcatcca
    36361 tcgaaagggc gccatagcga tcatgcaaca gttttttttg ttgttcaaaa ctctctctca
    36421 ttttccagat ctcctataca caaacttcca tcattatagc aaagttcttt ttggatgaaa
    36481 aatatttcat tgtttcatcg tttattgcta gttagaaatt tccataaatt tttcaacgcc
    36541 tcattctatt ccaaatagta tcagatatca agtaaaactt aagaaagata aaagtttctg
    36601 acaaaaggat gtttactttc ccattcctgc ctttcttgtt cttttatcac aacaaaaaaa
    36661 tggggctgcg acaaaaatct tgtcacagtc ccttattctg aataaacggt gtggaagaga
    36721 cagcctatca gtagtaataa gacacatctc tttcacagcc tcatttttgc gtaaaaatcg
    36781 atttattcga aaaattcccc gtatacatct tctacacgtt gatctggatt gatcacgata
    36841 tacaagttac ctgttttttc gctgattttt gttgattgat attgattgtc taatccttct
    36901 gttcctttat ctagatatgg gaagtacaca agatcaggtg tgccatttcg gtaaaggatt
    36961 tccattcgct cgatatcttc gtatttcaat gcgcgattaa acatgcccat ttccagtccg
    37021 cctaggttga tatcatgtgt cgcaactgcg tccccttcat ggaagatttc gattttaaat
    37081 ccagcacacg gatggatttc aacaaattca ctgccattga ttcgtccaaa acttgttgtg
    37141 atttgtttga tccaaagatc gccgatatga cggcggttga tcgtccaggt ttctccattt
    37201 ctaaaataaa attttagcgc tgtcatttct ttactcatta aaaagactcc tttatgatgt
    37261 aatttattct tgtactacct cagtttagca caaatttatg taagtggtca catcttatgt
    37321 tttttcttct aatgacgtat ctgcccactt taagtagcca acaaagctga gcagatacag
    37381 tctagtgttt tttaaatcaa tttttctttc gataaaaaat ccaaaacctg ttttttatca
    37441 ttttttattc tttgttctac gacggctttt tcagctaatt gcaaagcttc actaacccac
    37501 ttaccaggtt tttttccagt ttcttctagc aagattttac catctactgc taattcgctt
    37561 aatgacttga taggtaaacg atcataggat tctcctacgg catccatctg aggtgtttcg
    37621 tcaaaataga tcatcaattc ttctacttgc agtgcatgtt ttttgcctaa gaagtacaat
    37681 tcgaatggtt ccaaagctcc agtttctcgt tgtcgtagtc cttggacaag tgcttgaacg
    37741 tctttaatca tttggttaga acacttccac gatttcaaaa aggaacgaat ctcattttct
    37801 tttagatcca gttggctgat caataatgcc catgcttggc tctcttcttt caatgggacg
    37861 gatggtaatt cagcaaaacg taaaagtgct tcgccttctt ctttcaaacc gggacagcaa
    37921 atgtagcact ctgtttcaat aaatgcatgg attcccgcgg cccgttcatt tcccaagagc
    37981 atcttgatga attccacatt tatacgttct acagatattt tacctaatag ctcgtggttt
    38041 tctttgatgg cgtagaaggt ttcttcctct aatttgaacc ctagttgact gacaaaacgc
    38101 aacccgcgca tcattcgtaa ggcatcttca tggaaccgct catgaggatt gcctacagct
    38161 cgcaagatct gttcttctag atcttgcaga ccatcgaaca aatcgatgat ttctccatct
    38221 tctttcaaag caaacgcatt gatcgtaaaa tctcttcttt tcaaatcttc tgccaacgaa
    38281 cgaacgaatt cgacatgatc tggtctacgg tagtcctgat aagttgattc cgtccgaaat
    38341 gtggtgatct catattgttc atcattttct aataccaaca cagttccatg ttcgatccca
    38401 atatctatcg tttttggaaa aagctgtttg atttcttctg gaaatgcgct tgttgcgata
    38461 tccacatcat ggattggctg gttcagcaag atatcacgaa cacttccacc tacaaaataa
    38521 gcttcatatc ctgcattctc taattttttc agaactggaa ttgccttttg gtattccaga
    38581 ggtaattgtt ttaatttcat ttttccaacc tttcctgcgt gtttcttgtc ctgagataaa
    38641 gactcagttg catggtgact gtttgacaag tccgtttcag agtcttcaag gccaggacca
    38701 cttcatcttg tttactcgtc cttacgctca aagcgattat ggtctctttg attgaatttt
    38761 tccatgtttt tttcaaaaac ttcagttaag tcgatattca atgaattagc catgatcatc
    38821 gtaacgaata aaacatcacc aagttcttct gctaccgagt taactggttc agaagccttt
    38881 ttgctttttt ctccataagt gtggttgact tctcttgcca attcacctac ttcttcagtt
    38941 aatcgagcca tctgagctaa tggagagaaa taccccacct tgaattgctg gatatattca
    39001 tccacttcct gctgcatact gtataaagaa cgctttttat ccatcttttt ccctccaaaa
    39061 ctatcttacc aaaactttaa cgcaaatcct agataagagc aattgagaaa atcgaatttc
    39121 catgctacat ttaggaagga atttctaaat ggaaattctg tgaaaataca taattgaaag
    39181 ctggaattca tatggtcaaa cgaaaaacag taaaagaagt tctttttatc attataggaa
    39241 caagtattta tgcatttggt cttgtttact tgaacatcgc taatcattta gcagaagggg
    39301 gagtctcagg gatcaccttg atcttaagag ctttatttgg tattgatcca gcttatacga
    39361 ctttattaat caacatccca ttgattttac taggaggaaa gatcctcggt aaacgttcct
    39421 tggcctatac tgttctaggt acactttctt tgtcattctt tttatggatc tggcaaaggg
    39481 ttccactaga aatcaattta cagcatgatc tactgatcgt ttctctactt gcgggtttag
    39541 ttgcaggtgt aggaagcggg atcgtctacc gcatgggtgg aacaacaggc ggcagtgata
    39601 ttatcgctcg gattttagaa caaaattacg gcatcactat ggggcgttcc ctattggcat
    39661 ttgatatcat cgtcttactt gcttcattaa cctatatcga cttgaaacgt atgatgtata
    39721 cgttgattgt ttcgtatgtt ttcagtcgtg tgattgattc gatcttagat ggcggatatt
    39781 ctgcaaaagg tctacttgtc gtctccaaca aaagcgaaga gattgcacca ctactgatga
    39841 ccggtctgca aagaggtgtg actttccttt ctggagaagg cgcattctct ggtaattcaa
    39901 aaaaaatcat ctatatggtc gttagctcta gtgaattaac tgaagctaag aggatcgtcc
    39961 acgagattga cgaaaaagct ttcctttcaa tcatcaatgt gcatgaagtg gaagggcaag
    40021 gctttactta tttgaagcca cagacaagat tactgaaaaa actataagaa aaaagacaaa
    40081 gataggctat gaggaaagat tccacatcca gctgaccttt gtctttttta gtactatact
    40141 atttttaact aattgttatg ttcacttacc cggttacttt tcttttgtgt ctgcaaaagg
    40201 atctttcggt taacttcatc agataatttc aaaaaagcat ccctatctcc atcttctagt
    40261 gcttgatcga tttgagcata aagcattttt agttttgcct cttcttcttt ttcttgaaaa
    40321 tatcgttcaa ttaattcagt atcttcttca ctgactgaag aatcccaaga agaaaatgga
    40381 ttgtcttcta agatagatag atattctctt gtctgccaag cattttcgaa tatacattcc
    40441 acatataaag gctttttcca attcaaacgg atttcatgaa aaatctggtc tgtgtccgta
    40501 aattcttttc cctgcaaaaa caatttcatg ggttcccctt caacagatag atcactgatc
    40561 tgcagtcctc gatccgcaaa attcgcccct tcaacaaagt ggacattgct taaaatcgct
    40621 tcatgattgg caagataatt caagatccaa aaaacttctc ttcggctaaa agatacatga
    40681 ttgaccatcc acgttaaaaa attcttcttt tcccgtacat caattagcat ttttctcacc
    40741 tctctgttag gtcatctaat aatgacagtg cctccatatc tcctggttca agtgttaaat
    40801 aataagtcaa taattcttgc gctcttttca gctgcccttc ttctcttaag aaaaaggcat
    40861 actctttcaa gaaatctggt tcatgtgata gttcatgata agcctgttca tagtgtacag
    40921 ctgcaacggt aaagtcttct aattcattat aagcatgggc taggttccat tctgcataag
    40981 gattagcagt ttcttccatc tgattgatca cttgtatcac atcttcatat ctttcatcat
    41041 ccaagtaaag attacttaaa gtaagtaatg tttcatcttg tttgtctcct acttctaaag
    41101 ctttcagtaa aagttcttct gctcgctgtc tatcatgcag acggaaagca ttttccgagg
    41161 ccaggtgata aagttcgact tggtaaggat tttctttgat tccttcttct aacactgttt
    41221 gtgcttcttc aatcatttct tcttcttgaa gagcttgtcc tagatataga tataacgatt
    41281 gataatgcgg attaacttct cgaagttctt ccagatagtt gattgcctgc tgattttctt
    41341 tcaattgaag attcgtaaaa gctagctgga acagtcgatc atctgtcctt tcttctttca
    41401 gtgcttcttc taaaggtacg atagctgctt cgaattctcc ttgcatactt aaggattgtc
    41461 ctaaacgttc ctgcatcgag attcctgaaa tttcatttat cccgcttgcc aacaatgttt
    41521 cataaaccac tgctgcctct ttgaaacgat caactgaaaa atacaattca gctagcgcaa
    41581 actggatcaa tggttcatca ggaagcaacc cagctgcttc tttcaatttt gcttcactca
    41641 cttcagggat tccgataact tggtacaaat cagctatcgc caataagctt tgcggatagt
    41701 aatcactttg cttatccact ttttccaggt attcaaaagc taagtcgatc tcattatctt
    41761 cgattgcaat ttctgctaac gggatgttca gtccgtcatt ttctgggtat tggttgatca
    41821 gttgttcaaa aatctgtttt gcttcgatca aaaagccaat agccagcaat tcttcgccta
    41881 gttcagctaa tatatctgct gggtcttctt ttaatgcttt ttctaaaaag atttgtgctt
    41941 gtgacaagtc ttcatctgat aaagcttgca acattttttc gctatttgta ttcatttcaa
    42001 ttccctctta tctcactgac gatttgttga aataccgtca ttgttctcaa taattctttt
    42061 tcgtttggat gttggtatcc gtctattata cctatttttt ctaaaacaag tatttttttg
    42121 cttttttctg ccaaaaaaag aactttttcc aaatactcca tctttgaaat ctgttccagc
    42181 cagtggattg gatagccaag caagtccagc cacacaaaaa agttttttag ttggaaatca
    42241 aaaggatatg cttggagatt ccacacgaaa tgaaacatca ttcctaaaaa ttttttcata
    42301 ctagcagaaa gtagatggcc attttcggtc agataaaaag cttgttcaaa cagtttacca
    42361 taatttgaca ggttttctgc ctctaattga taaaaataga tcatttcttc gatcatcccg
    42421 gcaaacggcc gagtcgttaa ttgcttttta tttggatagt ttttatacag attttgcaaa
    42481 aaacgatgat cacaaatcat tccgcaacag ataaaagtca aaaagtcaat ctgctttccg
    42541 tccacctgcc gctcgctgat tgtttgatca taaacgatgc gttctggcag attgatggtt
    42601 tgcagcacta cacgatttgg tagctgcaaa atcgtccgct gcggtgttaa tgctcggctt
    42661 agagaacgga tcgatacagg gatcatccat agtgatgaat caagggttgt atgtttttga
    42721 aaaaaacctg ccagatccat gatcccttca tttccaaatc cgatgataaa catggattga
    42781 ttttcgggat agcgtttgac gaaatcaagc aggttcttta attctgttaa atgattgcat
    42841 tgagtgttgg cacaaatata ccagtcattg tttgcttgat ttgcaaataa cgggttgatc
    42901 ttatctgcaa acaaatcata atagcgctga ttcgttaaaa ataagattgg ctgatcaatg
    42961 gaaagttctc ttagctgact gactagcgtt tctccgtaaa acactttcgt caacgcatct
    43021 tctacttgat aatcaagttc catacgcatc cctcctcttc ttatttttat tttaccatta
    43081 gtaagcaaat tctagaaaag ataagagcaa agctcacctc ataacttgca aataaataaa
    43141 aattaaataa aatacatgtg ttttctatgt atgacatacg gattcggaat ctctgtctct
    43201 tatttattcc ttttctgcta ggaaataaaa agaaaagcac ttgtagccct agagccacaa
    43261 gcgctttttc aactgtctta tttaacagcg tcttttaatg ctttacctgg tttgaatgca
    43321 ggtactttgc tagctggaat ttcgatttct tcaccagttt gtgggttacg acctttgcga
    43381 gctgcgcgtt cgcgaacttc aaagttacca aaaccgatta attgaacttt ttcgccgtta
    43441 gctaaagcat cttgaatagt tgaaaataca gcatctactg ctgcagttgc atctttttta
    43501 gttaaatcag tagctgaagc aactttttcg attaattctg ctttgtttgc catggataga
    43561 ttcacctcct ccacaagaga tatgatagaa atatcattta gttaaaactt tctgagtggt
    43621 ccagaagttt aaaaagaaat attgattaac ttcaatattc tgttatcacg atatcatatg
    43681 aaccgcttaa tagcaaggtt tttaccaatt ttcacatgct ttttttagtc atagaaacgt
    43741 aacaaacgaa actgtctttt tcttttgaaa aaaaaacgtc gatatggtaa aatgcctatt
    43801 ttctcctacg agggataatt cggatggacg tcccttcaaa agtaaatgct tttctgattt
    43861 gattttctaa gaaacgttca taagaaaaat gcatcaattc ctcttcattg acaaaaacga
    43921 caaatgtagg tggtttgaca gcgacttgtg tagcataaaa aatcttcagg cgttttcctt
    43981 tatctgttgg tgttggattg attgctacag catccataat gatatcattc aacacagccg
    44041 aagagatacg aagattctgg ttcatactta cttgttcgat catttcaggc agacgttcca
    44101 accgttgttt agtcactgca gaaacaaaaa cgattggagc gtaatcaaga taccggaatt
    44161 catcacgaat ctcttgttcg aaatcacgca ttgtattggt ttctttcttt acaagatccc
    44221 atttattcac gacgataata attccacgac cagcttcatg ggcgtaaccc gctactcgct
    44281 tgtcttgttc ccgaatccct tcttcagcat tcagtaccat caaaacgata tcagaacggt
    44341 caatggcacg cattgcacgc atcacgctgt atttttcagt attttcgtat acttttccgc
    44401 gtttacgcat accagctgta tctatcatta agaatttctg accactttca gaaacgaagt
    44461 gggtgtcgat tgcgtctcgg gtcgttcctt caatatccga aacaattaca cgttcttctc
    44521 ctaaaatcgc attgatcaaa gaggatttac caacatttgg gcgaccaatc aaactaaact
    44581 taatcgtgtc gtcgtcctct tcttcagtct cttcagagaa atacttcact gcttcatcta
    44641 aaatatcacc aatccctagt ccatggcttc ctgaaacagg ataaggatcg cctagcccta
    44701 aggaataaaa ttcatagata tcgtttctca tttctggatt atctacttta ttgactgcta
    44761 aaatcactgg tttattactt ctgtataaaa ttttcgcaac catctcatct gcgtctgtca
    44821 cgccttcgcg tccgctgact acacaaatga ttacatctgc ttcttcaatc gcgatttctg
    44881 cttgatgctt gatctgatcc ataaaaggtt catcgccaag atcaattccg cctgtgtcga
    44941 tcacgctgaa ttctcgacct aaccattcac cttttgcata aatacgatca cgagttactc
    45001 caggagtatc ttcgacaatc gagatacgct caccagcgat gcggttaaaa atcgtagact
    45061 taccgacgtt cgggcggcca acgattgcaa ttgttggatt tgccatatta tttcctcctc
    45121 acaagtttcc aatctcactt agtttaacga atgcattgcc aactttcaag catagattgt
    45181 caattaaacg aagtattttt attccttatt gaaatactaa tcgcttttca aaagaaaaac
    45241 tgagacaaaa gcagctgctt tcatctcagt cacagataca aaatcaatta tttttcagaa
    45301 tcatcggttg ctttttcttc tacttcttct ttcaacgcat cacctaaaat gtctcccata
    45361 gtaaatccag tattttcttc tggtaattca taagactcaa cattttttgg ttcttcttct
    45421 gaagctggtt tttcttctaa agctttgata ctcaatgcaa tacgatgttc ttctggatgg
    45481 atctctaata ctttcacttt cacttcgtca ccttcatgaa gcacttcatg tggtgtcgca
    45541 atatgtttat gtgaaatttg agagatatgg actagccctt ctactccagg gaacacttca
    45601 acaaaggcac cgaagcttgt caaacgttta actgttccgt ctaatactgt gccaactgga
    45661 gcttttgtat cgatttctga ccaaggtcca ggctgtgtgt ctttgattga caaggaaaca
    45721 cgttcttgtt caggattgat agacaacact ttgacattta catcatcatt gacagccaat
    45781 acatcacttg gttttgctac atgactatgt gaaatctctg agacatggac tagaccgtca
    45841 attccgccta ggtcgacaaa tgcgccaaaa tcagtaagac gtgcaacacg tccactgaga
    45901 acatctccct catggatagt tgaaagaatt tcttttttct tttcttctct ttcgttttcc
    45961 actaccgctt tatgcgataa gatcaaacga ttttctgatg gttcgatttc gatgatttta
    46021 aagctcaatg ttttgccttt atagtctgaa aagtctgcaa caaaatgatc ttccaccatt
    46081 gatgcaggga caaaaccacg tacaccgacg tcaacgacta aaccgccttt gacaacattt
    46141 gtaacaggtg cttcgatgac cttgccctct tggaaatctc tttcgatatc ttcccaaact
    46201 tttttagcat ctaaacggcg ttttgaaagt agatagctgc cgttttcttt atctttgccg
    46261 atcgtactga taacgactaa atctaataca tcaccgactt ttactacttc gctgatatct
    46321 tctaccggca aagttgataa ttcttttgcc ggaacgactc cttcaactcc agccccttcg
    46381 attccgacaa tcacttgttt gtcttcaatg actaaaactt cgcccttaac aacatcgcca
    46441 acttttactt cttgtacgct tttcattgcg tcttccattg actggttgtt ctcttgattt
    46501 tgatcaaatt ctgtcataac acttgtcctc ctaaccaaca ttctgccgta agtacggtac
    46561 tgtttttagt ttaattgatt attcaaaaaa aataaagcga tttcgttcgt tttccttgaa
    46621 aaaacaattg tttttcattt tttttcttaa tttaaaaaag aagataacct tttttcaaaa
    46681 caacagcttt tattgctgca acgacttctg gaatcgattt gccagtcgta tcgatacgaa
    46741 cagcatcctc tgcttgcttc agcggagaga cgtcccttgt tgaatcataa tggtctcttt
    46801 cctgtatctc tttcgtcagt gtttcaagat cagtcggaat tcctttttct tggttctctt
    46861 tgaaacgtct ttctgcacgc tcacttacgc ttgctactaa aaagattttc acttctgcat
    46921 tcggtaatac agctgtcccg atgtctcttc cgtccatgac aacgccaccg cgttccccta
    46981 tttttcgttg gacagcaact aattcttctc tgacttttcc atgtgcagag acttcagaaa
    47041 cagcttttgt gacatctggt tgacggattt ctaaagtcac atcttttgat gctatgaata
    47101 catgctgtcc atcttcttgt tgcgaaaaag agataggata ctttttgatc agcgaaacta
    47161 gcccttcttc atctgaaaaa gatacattgt tcgtaatagc taaataagtt actgcgcgat
    47221 acatggcacc ggtatctgta taaatataat tcaattcttt tgctaatatc ttggcaacgg
    47281 tacttttccc agatgaagca ggcccgtcga ttgcaatact gatttgtgtc atgatataac
    47341 tcctttttct cagaaaaaaa gccgcaaagc gcggctatta ttatttaatt cttaattctt
    47401 gaccagggta aagcataaag ttgtttggat ctaagccatt caattcaaac aattgatcag
    47461 tggtgatccc tgcacgctca gccacttgtt ttggaccttc gcctgcttga acagtcgttg
    47521 ttgaaccggc tgcagctgat gaagaaggtg ttgtttctgc tgcattgctg cttgaatccc
    47581 ctgcttgata agttgcctca cttgaggaag tcggtgttgt ctctgctgca atactgcttt
    47641 ctgacgttac acctgtatct tgagacacag tagattcttc cgcgattgta ctagattcta
    47701 ctgttgaagt ctcagttgac tcctcttttg ttgtacttgt agaactttct actactgatg
    47761 tcgaagtttc agaagaagtt gctgctgttg cattgttgct tgatccattt ctgatccaga
    47821 aataagcacc tgctggaata gcgatacata agaccagtaa aatcaaaaat agcgaaagaa
    47881 ataatgtatt tcctttcttt tgttgtctct gagaacttct tgatgaaact tcatcatctg
    47941 tatcgtagat tggttgttcc catggttctt gtgtttcgtt attttctgta gattgatgtc
    48001 tatcttttct gctcactgtt ttacctccta aactctatcg tgtgtattat accataggaa
    48061 aaagtgtctt gtcctaaaaa atcaatgttc catgtttttt ttaaataaat ttagtaaaat
    48121 cgtttcccaa tgaagttgtt cagtcttaga aaaaagcttc ctctttggca gaagttctgc
    48181 ttttgctcca tcaatatcac aacaattctg cggcttttcg gagagttttt cgccaaaata
    48241 agctaagatg aattttctgc ggcagttttc ttcattaata tagcgaagca tctgctgcag
    48301 gcggaattct ttttcttttt cctgacgttt gagccgttcc acccaatttt ctggtttttc
    48361 agactgctgg atcagctcca tccatttttt ttgcagctca tcaaagggag cttgctttgt
    48421 ggcatattcc aaataaagct caaaactttg tcgctgttct ctagaaagtt gattaaagaa
    48481 ataatggata cgttcatccc cactttgata aaaaagaatt gctgcacttt cttcctgatc
    48541 tctgcctgct cgaccgattt cttgtacata gttttctaga ctgtctggca aatcataatg
    48601 gaccacataa cggatatcag atttatcgat ccccatacca aatgcattcg tagcaatgag
    48661 aaactgcagc tgatttttta caaactgctg ttgaagttgt cttctttgag ctgcatcaag
    48721 accaccgtga tagtatccaa ctgtaaaacg ttccctgaat aaatgataca atcgttcgac
    48781 ttcttttttt gttgcacaat aaatgattgc cgctccttgt gctctttcca tgaagtgttc
    48841 aagttcttgt tctttttgct gcgttttccg aacaaaaagc gagatatttt gccgattgac
    48901 tgattgtctg atctcttttg gtggtcgatc aaatagtaca tgacgtatat ctcttgctac
    48961 taaatctgtt gctgtcgccg ttagtgccag tgtgaccggg tttcctaaaa gctttcggat
    49021 ttttcctaac tgttgatatt ctggacggaa atcaactccc cattgagaca cgcaatgagc
    49081 ctcatctact acatataacg caatattttg ttttttcaga tgctctaaaa gagaaggatt
    49141 agtcaacatt tcgggactca aaaacaaaaa tttatattgc gatagatgac ggaggacgta
    49201 tcttctttca gattgcgaaa gtaaactatt gaaagcagct actcgtttct cccctcgttt
    49261 ttgtaattgc gtcacttgat cttccattag agaaagcagc ggtgacacga tgatgaccag
    49321 accttccatt aaataccccg tcaattgata gcaaaggctt tttcccgttc ctgtaggcaa
    49381 aatagcgaga gtatcttctc catcaagcaa cgcctgaata atttcttctt ggccgggacg
    49441 aaatgaatca aatccaaaat atttttttaa ttcatttatt aacattctgt atcgcttcct
    49501 ttctcaatag atagatttga tacaaacgaa attctccata atctaattgt ccatctacat
    49561 tccattcgct aaagcgccac tctcgcacgg aatccttatt tggttccaaa cgttcgattg
    49621 tttctgcaga gagaatccac tcgtatggaa aatcatcaaa aaacaaggcc cactcaataa
    49681 gatgatctgt tactgttcct ctttttaaat gacggattgc catgatctgg tcaattgtct
    49741 gaccattcat aaacatctgc ttggtgatca tcatgctttg attgaaattt tgctgtaata
    49801 atgaatcgat caaagcgtgc caaagcgaat ctggccgttt ttcgatttgt gctaacaaaa
    49861 ggtggataca tctgctttgt tgcagataaa cctctgactc atcattgttt tttgccagct
    49921 gataaggaag cagccctgtt cgctcatttc cagataattg gttagctaaa aaatctgctg
    49981 cttcttctgg tatctccgaa aacagctgca ccaaactctc atacgcagta tcaattaaaa
    50041 tttcacgtgg caattggctt tcactaagcc atttttttag ttggaaagta taaaaaggac
    50101 ttgtctctgc tggaagatag tcttgaatac ccgttgccag attagaaatt acttgaacag
    50161 caaatttaat gagccgccaa ctagtttccc ctgttctgcc atagcggtca aaacgcaaac
    50221 cagtatgttc tatttgatgt tcggacaaca tatcagcccc tgcaggcgtt agctttgctt
    50281 cattttcatt tatttcgatc caaccttgct gcgcaatttt ttgcaaaatt tgataaaact
    50341 tatcctgctt caattctgga aaactgttat ggataaacaa aagttcgtgg gtaaatccat
    50401 aaataagtac tgaagtcgtt cgttttccca caaggagttg gtaaagagaa ctaatccgca
    50461 gcttgtgtcc tgatgaaaat agcgataaaa taaaaacaat catattattc tccttaagtt
    50521 aggatggtta gaaatgtcac tactatgtaa acttgtacct gaacgctgta ttgcatgcgg
    50581 tctttgtcaa atcgaagcgc cagatatttt cgattatgat gaagaaggaa tcgttttatt
    50641 tgcccaagaa ccagaggctc atcaacagtt cgtgatgccg gaagaagaaa catatgtaaa
    50701 aaaagcgtat caaaaatgtc cagttcgagc gattttatta gaaaataaaa tataaatata
    50761 aacgaaactt taaaaacgta cactaagttc tctaaacttg aaaataagcc gggatctgtt
    50821 tctcttgttc cgtgaaatcc cgacttattt tgaaagattc atttgctatc tatagttaat
    50881 tgactagttt tttcacggac cctataggac ccatttatat tatttcaact ttttcagcca
    50941 gttttccaat gtattgacga tcacagtttg ctggtctgcg ttagaaattt ctgcctctcc
    51001 atctcctttt tgctcaccat agctgccaaa acctccatga tttccgccat tgattgatac
    51061 atactctgtt ctttccggta aatactgctt agcttcttga taagcttcct gattcaatac
    51121 tttatctttt gtcgccgtga tcgataaaac aggaacagct gattctgtca atgttccttt
    51181 tttgtcggga taacttgcca tgaagaatac cccttttaaa tgtttgtctg aatgattctt
    51241 attgacataa cgacttgcca ttacgccacc taatgaatgg ccaccgatca cataatcgga
    51301 atttggaaaa tcttctttga ccttatccgc tttgtttccg cccatcactg ctagatcaag
    51361 tggcattttg acaatagcga ctgaaaagcc gttttttgct aaatcttgcg cccaaatact
    51421 ataactttct ggctctacga gagcaccagg ataaaaaata agtaatggag actttccatg
    51481 attctttttc tctccttcaa aaaataagta atcttctttg ttttttcctt cttttaccaa
    51541 cacttgcgct tctgacgatg gttcgtacgt attttgctgt atatagtaat aaccggctcc
    51601 accgagtaat cctaaaatga tgactaagcc aattagtatt cgtctaaata ttttcacttt
    51661 ctcccacctt ttctatctga gtgacccctt tttcttaatc aagtatgatt ataccttaaa
    51721 aaaataagag agaaatgcaa actttccagc atcaaataaa aaaagacgga at
//
LOCUS       DAZKLD010000004        51032 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.004, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000004 DAZKLD010000000
VERSION     DAZKLD010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 51032)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51032)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51032
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.004"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            330..1175
                     /locus_tag="ACG8KR_000196"
     CDS             330..1175
                     /locus_tag="ACG8KR_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFQ0199605.1"
                     /translation="MDEMKLIGIDLDGTLLNSDQSISQKNARTMKHLPEDCFPFICSG
                     REVEDINNILKKSGLRIPAVGLNGSVGFDGEKKIFESSFDKNDVKKIDSVLSKFPTKI
                     YTNLGSYESQNYKNKMKKIFHEVGSEFSIKELNYELEYEKSICSIPYNNIEEIIEKDA
                     ISIYKFFIFIPNRQLKANIFNHLKKMLNVSVTESAPVNIEIVPQNVSKGVVFDRLESI
                     YNLKKPLRIAIGDSLNDLSMFESADISFAMGNSHQEIMKKADYVTVSNDKDGVSQAFE
                     RILTI"
     gene            1456..2676
                     /locus_tag="ACG8KR_000197"
     CDS             1456..2676
                     /locus_tag="ACG8KR_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HFQ0199606.1"
                     /translation="MVKFIHAADLHMDRSFEGLTTLDKTVQEKLLKTNLTVLSNIIEQ
                     AIINNVDFVLLAGDNFHQNRPSLKIQKHFSEQMKRLEKNHIPVFIIFGNHDFYQKERY
                     WFSFPKNVHLFTSETVETKKITIKSGETVSLSGFSYRQPWIQKDKVMEFPSRELTDYH
                     IGLYHGEPGVSQKGNYAPFQPSKMQEKGYDYWALGHIHVPTVLNEKQTIVYPGAPQGH
                     TKKEQASTSILLVELSKGSCQIHPIKVAEVYWKTKEISLRKARTTKEVIVHIRQQLSK
                     VEDGFSLIEIKLKDYDHLNTDVLERIQSGELLDYLLEEFSDRINDFFIWRISLIENTF
                     YTKTPLAASAKLMEQLFQYYQTPQDFQQILNEVYSHQEAARILSELPEYQEETLEKAK
                     QLLNQDFLFEEDQE"
     gene            2673..5393
                     /locus_tag="ACG8KR_000198"
     CDS             2673..5393
                     /locus_tag="ACG8KR_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFQ0199607.1"
                     /translation="MKILRIEIAAFGKWRQKSFDFYSGNQLIYGGNEAGKSTIYQFIQ
                     AILFGFPSKGRKKKDYTPKDGSAYGGKIWLKHPVYGEFAVERYKQQNRGKSKVWLGDQ
                     VGSDELLEKLIAPLTKELFQQVFTFQQEQLMELDHLREKELHDALISLGITGSSQVFQ
                     KRQEYYQKAQQLFKKKGQKLLINQKLNEWKELQEKIHQKQEQEAYFGKLIQQEQEYES
                     QQQQLLAQLKEANKQFSLARQQELNFSLYEEWQSLKKNSTTGLPDEQLLLDLESFSKR
                     YQQTNERLEELEATLEKHSGMDQQSARYYFYLEQEQELKDLQQLQFTAEQLNEEWQRL
                     QIETRETNSELKILEQRWHWQKNPLPEEFLYEEEWKHLLAEERRIADLESQISVRLQI
                     AKEQQEMIEAEVNDLEETYPALLQNQSEIAPVSSSKPYMLMAGGAIIIAGIFVPMPLK
                     VILLLIGAGMIGYFFYKRQPKIKTKNDFSEIKGMWQEKLGQLDLALAQLAEAEEKQKR
                     TGEEKQILQQEVYRFADHRHLGKMDTLKLMKEYGQEVIDYQKLYGVFERKHKREQEIQ
                     EKLRQIDQKFEFVKEWLPIQDKTISEKMVLLSRFIQEMEQMKFARSYQQNTLLKQEIN
                     QLKKKQKVWIQEYSEQLTQAGLSYPSEVPVFLQKAKQAEQQMKRYNELSEMLTPLFPE
                     ELTKEKLSVRLRQLKEKQSQLQQEEKEASENRQRLQLQVEELQRDGTLDELYQQASRQ
                     KAKLQELLEEWGALEIAGTFLGDLSTEMSERQLPQLLRAAGDYLGILTKGNYQKILLK
                     NDTLTLTDGYTEFPIYELSTGTKDQLIMAMRFAYLSLEANRSICPIIIDDGWLHYDHQ
                     RKYQLAELLAKFGEKYQIICFSSDKEMVSYYHEMNQIVKQLEGANNEKNS"
     gene            5377..6321
                     /locus_tag="ACG8KR_000199"
     CDS             5377..6321
                     /locus_tag="ACG8KR_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFQ0199608.1"
                     /translation="MKKIRDLVIDEQFEQFVLIKSADVRIAKNGKKFIAFTFQDTSGT
                     IDGKYWDASEEEINKFTAGTVVFLNGKREVYQGNPQVKILHMRLTRVDEPGEPSLYME
                     RAPLKKEEMEEEINQLLFEITNAHWNRIVRYLLNKYQKQFFEFPAAKRNHHAFASGLA
                     YHTLTMLHLGKSIADEYPELNRALLYAGIILHDLGKVIELSGPMSTEYTLAGNLIGHL
                     VLIDEEITKACLALKISEADEDVLVLRHMVLAHHGLLEYGSPVRPRIMEAEILHQIDN
                     IDASMQMMLTSIRQTEPGKYTDRIFGMDNRSFYVPKDL"
     gene            complement(6390..7400)
                     /locus_tag="ACG8KR_000200"
     CDS             complement(6390..7400)
                     /locus_tag="ACG8KR_000200"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFQ0199609.1"
                     /translation="MKKKSIILAATSALAVLTLAACSGDTNKDIATMKGGTITVSDFY
                     DEAKLESSNQSLVQRMIIYKVFNNKYGDKVTDKQVDAEYDKQAKSLGDTFESQLEAAG
                     YTKDTYKEYIRNNLAFEAGLKAHVDITDDDLKTAWKSFHPEVEAQIIKLSSEDEAKDV
                     KKSADDGDDFSKLAKDKSTDTETKEDGGKVKFDSTTTTIPAEVKEAAFKLKDGEISDV
                     ITTTNPTSYATEYYVVKMVKNQNKGNDMDKYKDQLKDIATETKLSDNAFTTKVIGEEL
                     KDANVKIKDDAFENVLSAFTTTSSSTKDSSETTASTKSSDTKSTDSTKESSTEETTDS
                     SK"
     gene            complement(7580..7924)
                     /locus_tag="ACG8KR_000201"
     CDS             complement(7580..7924)
                     /locus_tag="ACG8KR_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HFQ0199610.1"
                     /translation="MIKNFFKGLLFGAAAGTVGGLLLAPRSGNETRKKLIEELEEATT
                     LTNDLNNSLNHFKSALIETKETAESVIPAFQEAIKKDIEDFKFQAEPRITQINEQVEK
                     LKADLPEYPEQI"
     gene            complement(7917..8351)
                     /locus_tag="ACG8KR_000202"
     CDS             complement(7917..8351)
                     /locus_tag="ACG8KR_000202"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HFQ0199611.1"
                     /translation="MENCIFCKIINQEIPSYKIYEDDKVYAFLDISQATKGHTLVVPK
                     RHVADIFEYDPELAGEVFSRVPKIAQALEKAFPEMKGLNILNNNRELAYQSVFHSHIH
                     LVPRYSKEDDFSIHFVNHQDSYGSEELKAIQETIVKQVSCDD"
     gene            8489..9226
                     /locus_tag="ACG8KR_000203"
     CDS             8489..9226
                     /locus_tag="ACG8KR_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0199612.1"
                     /translation="MSLIIDHVTGGYGHLPVLKDISFDVQPGEIVGLIGLNGAGKSTT
                     IKHIIGLLEASKGTIEINHLSLKKNTEEYRKQIGYIPETPSLYEELTLREHIEVTAMA
                     YDIPMDIAMKRAEKLLETFRLSNRLDWFPANFSKGMKQKVMILCAFLIEPSLYIIDEP
                     FLGLDPLAINALLELMVEMKKEGAGILMSTHILATAEKYCDRFVVLHNGEIRADGSLE
                     MLRKEFQLPDSSLDEIYIALTKEQAHE"
     gene            9219..10430
                     /locus_tag="ACG8KR_000204"
     CDS             9219..10430
                     /locus_tag="ACG8KR_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0199613.1"
                     /translation="MSSFFQKRLARHQKKMMKYMRYVLNDHFVLVCLFLIGGVGLYYS
                     NWLKTLQPPFAIGGLIISVFWMLCLFVGKMATFAEAADIVFLLPKEKEMQTYLERAFR
                     YSCIFPLIFLILACGLAMPLVVVSTEQSFSMFFVYLIILWSLKFSHLQIKRAELFRLD
                     QQVIRYWKLGWFFSSLIFLFLSFYWLIWAGIAGALVQASLYYWFLWKQQEKGLDWEKM
                     VHVEEHRLHQIYQFINLFTDVPEITAKIKRRRYLDPILTHIKRESKNTYLYLFARRLA
                     RGSDFSGLYFRLVIIGGLLIAGVVDFYFSLIIGFLFLYLIGFQLLPLANQFRYMVLVQ
                     LYPIHKNQKKQAIQYLLTWVLIIASVIFGLIAAVVLDGQQRWVPLAAYLIVTMLFVKL
                     YVPTRLKKMSD"
     gene            10634..11416
                     /locus_tag="ACG8KR_000205"
     CDS             10634..11416
                     /locus_tag="ACG8KR_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HFQ0199614.1"
                     /translation="MMEFQLDQEWRLRPIKGDTGKTYIGMRDEDKVFIKRNTTPMLAA
                     LSREGITPKLVWTKRTGNGDTLTAQEWLEGRLLTPNEIGQRNDVIDVLYHLHHSNSLK
                     TMLTRIGGRVMTPEKMLQKYEKELPEELQKNQFLQIVHRFLREQIPSIDKENLSVVHG
                     DVNHRNWLVCKNYLYLVDWDSIMFADPAVDIATILGNYVPLSSWNQWLISYGIRPTDE
                     MLEKFHWYAAMNLLQEITRYCKREDHRRMNEEILQLKRIFSG"
     gene            11486..12148
                     /gene="trmB"
                     /locus_tag="ACG8KR_000206"
     CDS             11486..12148
                     /gene="trmB"
                     /locus_tag="ACG8KR_000206"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HFQ0199615.1"
                     /translation="MRVRNRPGAAEMLAAHPNFVISDPTLWKGKWNELFENDHPIHIE
                     IGMGKGQFITGMAKAHPEINYIGVEMQVSVVSIALDKLIEQPLPNLKLLHVDGSALTE
                     YFADSEVDQIYLNFSDPWPKKRHEKRRLTYKTFLAVDEQILRPNGEIHFKTDNQGLFE
                     YSLASFSQYGMILKQVWLDLHQSQFEGNIMTEYEEKFSSKGQRIYRVEAQFQDKTKKK
                     SL"
     gene            complement(12246..14000)
                     /gene="ade"
                     /locus_tag="ACG8KR_000207"
     CDS             complement(12246..14000)
                     /gene="ade"
                     /locus_tag="ACG8KR_000207"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000034 - adenine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006146 - adenine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HFQ0199616.1"
                     /translation="METTLRKYIEVASKKKPADLVIKHAKIVNVFTKEIMEKDVAICE
                     GMIVGIGEYEGKSVHNANGQYLVPGFIDGHVHIESSLLSPSEFSKVSLLHGVTTVVTD
                     PHEIGNIAGSTGLEFMIEDARQTLMNIFFMLPSCVPVTPFETNGANLNAKKLAPFLQK
                     PEVLGLAEVMNYQAVANNETDILEKIQLMHQHKKKIDGHAAGIGMEELNVYPAAGIRT
                     DHEATTAKEAKERLDLGMYLMVREGTVAKDLASLLPAITPENSRRCFFVTDDKLINDL
                     VKEGSIDHIVRQAIALGIDPLQAIQMATLNAAECFDLKTLGAIAPGYQADFFLTDDLT
                     KLPIHEVFYKGTLVVQSGQLQDHLFADHSNSYTCCLPKLNAQPLNPHSLELLLSSSQA
                     HVIEIIPNSLVTKDLVEKVDRQGERFIPSIEKDQLKIAVIERHHQTGNIGLGIVKGFQ
                     LKQGAIATTIAHDSHNLVVVGTNDEDMLYAANLLIQKGGGMTVVNKQQEIACVSLPIG
                     GIMTQEPFSIVYEQLNELITKAHQLGAAKTFDPFLTLSFLTLSVIPELKITDKGLFSF
                     SRFQLIPPCGKTDEINTF"
     gene            14132..15061
                     /gene="comGA"
                     /locus_tag="ACG8KR_000208"
     CDS             14132..15061
                     /gene="comGA"
                     /locus_tag="ACG8KR_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus ATPase ComGA"
                     /protein_id="HFQ0199617.1"
                     /translation="MEIKRLSDQMISESFAKHAQDVYILPASEVFYIYFRYGKKRTFW
                     KKVSSADGQQLISRFKYLGQMDIGEKRKAQLGAFTYSLEKQEVRLRLSSVGDYLQRES
                     LVIRLLYGTFETPHRCFSSRDFATILQKTVRRGLYLFSGPVGSGKTSLMYRLALEEKL
                     QVITIEDPVEIEEPFFLQLQINPKIAQTYDTLLRLALRHRPDLLIIGEIRDESTAHAA
                     VRAALTGHRVFATVHARNLEGTLVRIHELTKKKEELSECLAGVIYQELLEDANHHPSV
                     LWSYQFMEEESVKKSWEESYEEAQENIWQSELF"
     gene            15018..16067
                     /gene="comGB"
                     /locus_tag="ACG8KR_000209"
     CDS             15018..16067
                     /gene="comGB"
                     /locus_tag="ACG8KR_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus assembly protein ComGB"
                     /protein_id="HFQ0199618.1"
                     /translation="MKKLRKIFGSQNYSKGLTRSQQNQFAQTMGELLLSGFSLQESLY
                     LLLTIRSFPPVILANIQHQLKEGQAFSEVLSQIGYGAEQLVQIELAERHGNLPTTLLE
                     IAKQFRLVEGFRNDLKKLLAYPMLLLTFLLGILFSMRLFVLPQLIGSGMVMQEHWGIQ
                     LIQVFHWYVLLFILAVTLLALLVRYRLMKLSPVDQSEWISRQLLIGPIYSLYQSAYLS
                     LEIGKLFHEGFELRQIIVCLKETKQESLIQHLASKLIQGLEAGMSLAEQFQNYRFLSE
                     EFSKIVLQGEAKGSLGKELIFYSEWTRKQLFKRLHHWMHFIQPIIFLGVAALILLVYA
                     AVLLPVYGSIEEVLP"
     gene            16064..16342
                     /gene="comGC"
                     /locus_tag="ACG8KR_000210"
     CDS             16064..16342
                     /gene="comGC"
                     /locus_tag="ACG8KR_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus major pilin ComGC"
                     /protein_id="HFQ0199619.1"
                     /translation="MKKRKISYYSGFTLIEMLIVLLIISVLVLLFVPNLSRYRNHVDQ
                     ESREAIIQLVDTQKELYALQNNGRVPTVEELLNEGYIKREHAEIYQRP"
     gene            16317..16787
                     /gene="comGD"
                     /locus_tag="ACG8KR_000211"
     CDS             16317..16787
                     /gene="comGD"
                     /locus_tag="ACG8KR_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGD"
                     /protein_id="HFQ0199620.1"
                     /translation="MQKSISGLEGKVLASGYTLFESLLLILLLTTILSFPVLAFSAWK
                     NELAVYQFFGQFEKRIYSTQKIAIVNQIQTGFYWNSEENQIIFHVPNPDKINWEVLDI
                     PDEITLKRHASITFAARTGNESSLKAYQFYWEEKERTITYQFQIGSGRYTKRIE"
     gene            16759..17106
                     /locus_tag="ACG8KR_000212"
     CDS             16759..17106
                     /locus_tag="ACG8KR_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0199621.1"
                     /translation="MVAIQKELNKTGFVLLESLTALMIASVCVYLVSFGQLFLLQAEK
                     KSGEELLFIRMIYEDIKTQRMYHKRPERREEMIPYDIQIVDNNGVIEKVFVNMEKEAI
                     SIEKTSNSSIYAD"
     gene            17069..17494
                     /gene="comGF"
                     /locus_tag="ACG8KR_000213"
     CDS             17069..17494
                     /gene="comGF"
                     /locus_tag="ACG8KR_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGF"
                     /protein_id="HFQ0199622.1"
                     /translation="MKKQVIPAFTLIECLIGLMILSSLLLTFNLLIQQTIQVSEFLQR
                     KDQKEWLIFLAQLEEELKSSHNVSVKNQQLHYTIEDKQYIIERYQAMIRKRRTSGGHQ
                     PMLTSITELQLLEKEHTISFYVHFENGEDGYAIWTKNDQ"
     gene            17466..17825
                     /gene="comGG"
                     /locus_tag="ACG8KR_000214"
     CDS             17466..17825
                     /gene="comGG"
                     /locus_tag="ACG8KR_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGG"
                     /protein_id="HFQ0199623.1"
                     /translation="MPYGQKTISRKCYKGSILFSVLLLFLLCTFLLMAALESYRLSMD
                     LNIRIKNYYIAKIMKQMALSEQSTLAESSEGVFYYTTGSVTYQWVQQSLFLEVEVSPF
                     IFRFTEEVKNDTDTSTE"
     gene            17997..19004
                     /locus_tag="ACG8KR_000215"
     CDS             17997..19004
                     /locus_tag="ACG8KR_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFQ0199624.1"
                     /translation="MLPEKMEQGFNQNLEAIQLLQNALGTSFLEAYVENAENLIDDYQ
                     VRVVDGVPTKETTQRITALYEELKKLSFEPEEWRRLSQLLLLKGSQTEHLQANHQLTP
                     DSIGFLFVFLIEQLYTNKKAPVKILDIAAGMGNLLLTVLLNLSNAGYQTEGIGVDIDD
                     TLLAVAASTSDLTQANVQYFHQDGLQELLIDPVDFAISDLPIGYYPNDEKAKEFLTST
                     EEGHSYAHHLLLEQSMKYVKPDGFGLFLMPSGFLETDQSEEIKKWFKEEGYLQGMIQL
                     PDELFRNKQSQKSILILQKKGPQAQQVKEVLLVKLASLKEPEKVTEFFNEFKNWKSSA
                     L"
     gene            19029..20213
                     /locus_tag="ACG8KR_000216"
     CDS             19029..20213
                     /locus_tag="ACG8KR_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate/propionate family kinase"
                     /protein_id="HFQ0199625.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEETVVAKGIVERIGLQDSIFTI
                     KYGDNQKFEQIMDIENHEVAVKMLLDQLIELQILSSYDEITGVGHRVVHGGETYGDSV
                     VIDEKVMDRIAELAEFAPLHNPANLMGIKAFKKILPDILSVAVFDTSFHSTMPKQNYL
                     YSLPVSYYEDFKVRKYGFHGTSHRYVSERAAKLLNKPIEELKIITCHLGNGVSITAVD
                     GGKSMDTSMGFTPLAGVTMGTRSGDIDPAVLPYLMEKLEIDIDEMINVLNKKSGLLGL
                     TGLSSDMRDLEKNYEKEHIRLAYDIFVDRIRKYIGGYVTTMNGVDAIVFTAGIGENDG
                     HVRSEIIKGMSWFGCEIDPALNELRGEEVDISTKESKVRVLVIPTDEELMIARDVERL
                     RG"
     gene            complement(20650..22233)
                     /gene="abc-f"
                     /locus_tag="ACG8KR_000217"
     CDS             complement(20650..22233)
                     /gene="abc-f"
                     /locus_tag="ACG8KR_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protection-like ABC-F family protein"
                     /protein_id="HFQ0199626.1"
                     /translation="MIIQAISIKKTIGGNPLFEDLSCQIEAGEKIALVGINGTGKSTF
                     LQILTGREGVDSGVISRKKGLKIGTVEQELTVSEATVNHYLLHSAAEIQDLKQQMSRY
                     EALMTEPDTNLEKCLARYGELQHRFEELGGYVLEDRIAAILQGLGIPHRQEARLTELS
                     GGERVKVALAKILAADADLLLLDEPTNHLDLNSIRWLENYLQNTKKSVLVVSHDRQFL
                     DQVTTKTWELEEGALIEYPGNYSRFRVLKEARLAELTKNYELQQKEVQRLKVMIRRFR
                     QWAHEGDNESFFKKAKELERRLAKLTLVKPPPPPKNRLQSLSNGGKSGKEVFIIQNLH
                     QQYADQVLFKDSSFAVYRGDHLAIIGDNGAGKSTLLKLLLGEEKPYGGTIKLGSSLQI
                     GYLPQQLQFADPDSRLLAYAITLTGNEEVARRQLAKSGFYQSDVGKRIKDLSGGEKIR
                     LALLKLFLEKINVLILDEPTNHLDSYAREEIEEMLQDYQGTLLAVSHDRYFLQQHFQE
                     ALVIDQEQISRQPLGQLID"
     gene            complement(22338..22466)
                     /locus_tag="ACG8KR_000218"
     CDS             complement(22338..22466)
                     /locus_tag="ACG8KR_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0199627.1"
                     /translation="MMRMTKITSNENYQRYHIAFSHAIGFTVAVDGTSLSKTVEFT"
     gene            complement(22811..22954)
                     /locus_tag="ACG8KR_000219"
     CDS             complement(22811..22954)
                     /locus_tag="ACG8KR_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HFQ0199628.1"
                     /translation="MEVSDESDTKKYEVSMADTDFIAKLKFYFGFGYREKASATFGKS
                     FKN"
     gene            complement(23177..23656)
                     /locus_tag="ACG8KR_000220"
     CDS             complement(23177..23656)
                     /locus_tag="ACG8KR_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFQ0199629.1"
                     /translation="MLQQYKKIMVAVDGSDEAELAFKKAVNVAIRNNGELLLAHVIDT
                     RSFQTVSSFDGMLAEQATEMAKQTLADYESNAKKAGLNNVTSVVEYGSPKQIIAREIP
                     EDNQVDLIMLGATGLNAVERLFIGSVSEYVIRNAACDVLVVRTDLENQLPAKDHTKA"
     gene            complement(23729..24013)
                     /locus_tag="ACG8KR_000221"
     CDS             complement(23729..24013)
                     /locus_tag="ACG8KR_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0199630.1"
                     /translation="MIRILMIIATLVLLFVSYYLFNKQDIFFVLINKNDKNQGFLQFY
                     GAAYAVLGVMGILTAFFNQRFIALVFLLIVIIVSATFSIRFAKKIAEPKQ"
     gene            complement(24091..24285)
                     /gene="ssrS"
                     /locus_tag="ACG8KR_000222"
     ncRNA           complement(24091..24285)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="ACG8KR_000222"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(24357..25643)
                     /locus_tag="ACG8KR_000223"
     CDS             complement(24357..25643)
                     /locus_tag="ACG8KR_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HFQ0199631.1"
                     /translation="MQQPLAYRMRPRTIDEVVGQQHLVGEGKIIRRMVDARMLSSMIL
                     YGPPGTGKTSIASAIAGSTNYAFRMLNAATDSKKDLQVVAEEAKMSGTVILLLDEVHR
                     LDKTKQDFLLPHLESGRIILIGATTENPYITINPAIRSRTQIFEVKPLTEQDIQLAVE
                     HALRDKERGLGQQAIQLDEDALLHLSRATNGDLRSALNGLELATLSTPSDKEGRIHLT
                     LSIIEECVQRKALTHDKNGDAHYDVISAFQKSIRGSDVDAALHYLARLVEAGDLASIC
                     RRLMVIGYEDIGLGNPAAAARTVNAVLAAEKLGLPEARIPLADVVVDLCLSPKSNSAY
                     MALDAALADIREGKAGDVPDHLRDSHYKGAKSLNRGVGYQYPHHFDQAWVNQQYLPDK
                     LKNAQYYQPKDTGKYEQALGQQYYRIKEWKKHPPKN"
     gene            25781..26452
                     /locus_tag="ACG8KR_000224"
     CDS             25781..26452
                     /locus_tag="ACG8KR_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HFQ0199632.1"
                     /translation="MTDKQKIDEFFWNKTTPEVARDLLGMYLEHDTPEGRLAGYIVDA
                     EAYLGPEDEAAHSYGLRRTPRVRAMYEKPGTIYLYTMHTHRILNIITQPEGIPQGVMI
                     RAIEPAAMIDQMSKNRGGKTGPDISNGPGKLVEALAIPQELYGQSIADSSLRLVFEKK
                     KTPKKINALPRIGIPNKGVWTEKPLRFVVSGNPYISLQRKNQIEKDWGWRKENEKEGS
                     INIFR"
     gene            26418..26906
                     /locus_tag="ACG8KR_000225"
     CDS             26418..26906
                     /locus_tag="ACG8KR_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HFQ0199633.1"
                     /translation="MKKKDLLTYLDEIIEKQVTDYDVALDWDTKNHTIEIVVRLFAEN
                     KAQFEIDDAEGIVSQEPIIEFEDGVLLFNPQKSVFDERNYLAVIPYEGKKGLAKSVAD
                     SLVEYLNEVLAQGQSDLLDFLDEDDDEAVFELHWDNQQLAELVEVKQQNEADTYLPYP
                     SY"
     gene            26919..27866
                     /gene="prmA"
                     /locus_tag="ACG8KR_000226"
     CDS             26919..27866
                     /gene="prmA"
                     /locus_tag="ACG8KR_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HFQ0199634.1"
                     /translation="MKWNEVKVETESEAVEAVANILMEAGASGVAIEDSLDVEHFQSD
                     QYGEILDKNDFTSLKEGALVMAYFPETTFLPEILPFIKDRITQLPEFGLAIGKNKVTV
                     SEVEESNWATAWKKYYHPVRITRMLTVAPSWENYQTEDPLEKIITLDPGMAFGTGTHP
                     TTSLTLTALETVLRGGETLLDVGTGSGVLSIAAKYLGAKDVYAFDLDEVAVRSAKENM
                     DMNEIAKDVHVSANDLLTGIEIESDVIVANILADIILLMIPDAWRLLKQTGTLIVSGI
                     IEEKKQMVLDAMLEQGFIVDQIFQQKDWYAIILKKPEVE"
     gene            27867..28631
                     /locus_tag="ACG8KR_000227"
     CDS             27867..28631
                     /locus_tag="ACG8KR_000227"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HFQ0199635.1"
                     /translation="MQRYFLKESYESKDYFVLEGENYHHIVRVMRMEPEDEVFLAFND
                     QIAIVAEITEITEEAVYLKEIKKEENQKELPSSITIASGYPKGEKLDWIVQKSTELGA
                     QAFLGFPASASVVKWDAKKRMKRQQRLEKIAQEAAEQSHRQIVPSVELLETKQDLFER
                     LDDYDVVLVAYEESAKQGEKSNAAKILSETKPDSRILAVFGPEGGLSRDEVDYFVEHK
                     GILCGLGPRILRTETAPLYLLSAASYQWELMNPSFS"
     gene            28864..31077
                     /locus_tag="ACG8KR_000228"
     CDS             28864..31077
                     /locus_tag="ACG8KR_000228"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HFQ0199636.1"
                     /translation="MTKEEILTGPGVIKLVSQYMGPEHVAFVEKACEYATAAHDGQFR
                     KSGEPYIIHPIQVAGILADLKMDPHTVATGFLHDVVEDTEITLEDLREEFGDDVAMLV
                     DGVTKLGKIKYKSHEEQLAENHRKMLLAMAQDLRVIMVKLADRLHNMRTLKHLREDKQ
                     RRIAQETLEIYAPLAHRLGISRIKWELEDTALRYLNPKQYYRIVHLMQTKREEREKYV
                     SGTVEDIRIATEELGIFAEIYGRPKHIYSIYRKMKDQKKQFNEIYDLLAIRVIVDSIK
                     DCYAVLGAIHTKWKPMPGRFKDYIAMPKANMYQSLHTTVIGPAGNPVEIQIRTQEMHE
                     IAEFGVAAHWAYKEGKNEKVEPDGMTKQLSWFHEILELQDESYDASEFMEGVKGDIFS
                     DKVYVFTPKGDVTELPKGSGPLDFAYSIHTDIGNKTTGAKVNGKMVQLDYKLKNGDII
                     EIMTSPNSFGPSRDWLKLVATSKARNKIKRFFKAQDREENVIKGHESVVKCITDLGFT
                     PKDILTKNKLQEALDRFNYQTEDDLYAAVGYGEVSPLTMANRLTEKERKEQKIEQQKQ
                     EAEEIMNQPKKEPDKMKVRHEGGVVIQGVENLLIRISRCCNPIPGDDIVGYITKGRGI
                     SIHRRDCPNVQPDKPNVAERLIEVEWEDTSNTRKEYDADLEIYGYNRSGLLNDVLQTV
                     NALTKNLNSVEARTNKDKMATIHLTVGIQNLSHLKSIVDKIKAVPDVYSVRRTNG"
     gene            31088..31534
                     /gene="dtd"
                     /locus_tag="ACG8KR_000229"
     CDS             31088..31534
                     /gene="dtd"
                     /locus_tag="ACG8KR_000229"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HFQ0199637.1"
                     /translation="MRAVVQRVSKAAVSVDQQEIGKIGYGLLVLLGIHETDTQKDVDY
                     LVKKIAQMRIFEDEQGKMNISVEDVGGQILSISQFTLFADTKKGNRPSFVEAARPETA
                     IPLYESFNEGIRSRGITVATGQFGADMSVSLVNDGPVTIIIDSKNK"
     gene            31705..32964
                     /locus_tag="ACG8KR_000230"
     CDS             31705..32964
                     /locus_tag="ACG8KR_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HFQ0199638.1"
                     /translation="MKEVPVVDIKITDLKKLYQKGYNMLEELRHEADAPVVKAKIFNK
                     EAITIYGSSAAKVFYDPRNFKRKGAMPKLVLKTLFGQGGVQTLDGAAHHHRKNIFMDL
                     MTPERMEDYHRILDKNLTQALEAQHGQFELFDLSKMVFFTSICEWAGINLSAISKDEV
                     EKLAEYQISMISGTFTSPIDHIKGVENRKKSEKWAQGLIEEARQNPVAGKENVALYAF
                     ANATDLDGQLLPLEVAAVELLNIIRPTVALTVWAALMGHALFSRPDLYQQLKNDFSTL
                     QDPFIQEMRRYYPFFPMLPAISLKEVEVDGYRIPEGSWVILDLYGTDHDERTVEAPDS
                     FMIKRYVGKAKDISYKEEYEMIAQGGGNFRQMHRCAGEWITLHSLRVFSDQLVNKFEF
                     SVPEQDWTIPFNQFPTYPNSRALLYKN"
     gene            complement(33053..34000)
                     /locus_tag="ACG8KR_000231"
     CDS             complement(33053..34000)
                     /locus_tag="ACG8KR_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFQ0199639.1"
                     /translation="MAEIRIGHSQVYAEQLGLGANAVGGHNLFDGLEDETGKQVVRTA
                     LNSGINLIDTAYAYGNGRSEELIGEVLKEKEYDRSRVVIATKAAHVPNKGRTFDNSPE
                     FLKQSVEDALKRLQTDYIDIFYIHFPDESTPKNESVATLHELKEAGKIRAVGVSNFTL
                     EQLKEANADGYVDVVEDKYSLIHRQAEKELFPYLEKNKISFVPYFPLASGLLTGKYEL
                     GEEKQFGEGDPRKRNPDFQGERFREILTAVDVLRPIAKRYQATPAQLVLAWYMKNPRV
                     SVVIPGAKRPEQVSDNVQALDLHLSNEDYQTIDEAFRGF"
     gene            34394..35686
                     /gene="hisS"
                     /locus_tag="ACG8KR_000232"
     CDS             34394..35686
                     /gene="hisS"
                     /locus_tag="ACG8KR_000232"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HFQ0199640.1"
                     /translation="MSFQRPKGTNDILPGESEKWQFVEETARLLFRDYQYNEMRTPIF
                     EHYEVISRSVGDTTDIVSKEMYDFYDKGERHVTLRPEGTAPIVRSFVEHKLFGPEYAK
                     PFKTFYMGPMFRYERPQKGRLRQFHQIGVEAFGSENPATDVESMVMALDFFKQLGITQ
                     IRLVINSLGDKETRQAYRQALIDYLLPFESELSEDSKRRLHENPLRVLDSKDKRDQKF
                     VAEAPSILDYLSEPAQKHFDTVVSMLDALAVPYEIDSNMVRGLDYYTHTIFEIMSDAP
                     KMGAQATICAGGRYDNLVEELGGPSTPGFGFAMGIERLLLTMEAEEVVIPAFNELDAY
                     VVALGDETNIEALKVVQAIRNFGFSADRDFMNRKAKAQFKTADKANAKLVLVIGEDEL
                     ASQTINVKAMASRKEKSFPLEEIYEHFDEVYDEMTLFE"
     gene            35701..37470
                     /gene="aspS"
                     /locus_tag="ACG8KR_000233"
     CDS             35701..37470
                     /gene="aspS"
                     /locus_tag="ACG8KR_000233"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HFQ0199641.1"
                     /translation="MAQRTIYCGLVSEQQLEQTVTLKGWVQKRRDLGGVIFIDLRDRE
                     GIVQIVFNPEINKEAWEIADKCRSEYVIEVTGEVRRRGENAVNPKMATGEFEVIASEI
                     VILNQAKTPPFQIEDDQAISDEIRLKYRYLDLRRPKMTKNIKLRNATTKAIRHYLDDN
                     DFLDIETPYLGKSTPEGARDYLVPSRVHAGHFYALPQSPQLFKQLLMNAGFDRYYQIV
                     RCFRDEDLRGDRQPEFTQVDIETTFLTAEEIQTYTEGLIAKVMKEVRGIEVTLPFPRM
                     TYDEAMARYGSDKPDTRFDMELIDLSDTVKEVEFKVFQMALENGGVVKALNAKGAADR
                     YSRKDMDQLGQYVGQFGAKGLAWLKVEEDGLKGPIAKFMGEATEAIIKATDAKPGDLL
                     MFGADKSEIVAAALGAIRTRLGKELGLIDESKFNFLWVTDWPQFEFSEEEGRYVSAHH
                     PFTMPKEEDIPLLAEDPAKVYAEAYDIVLNGYELGGGSIRIHTRELQEKVLETLGFTK
                     EQMEEQFGFLLTALDYGFPPHGGIALGLDRFAMLLAGEDNIREVIAFPKNGKAADVMS
                     DAPSMVSPLQLFELNIDVTNIEE"
     gene            37579..38454
                     /locus_tag="ACG8KR_000234"
     CDS             37579..38454
                     /locus_tag="ACG8KR_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFQ0199642.1"
                     /translation="MKKTFENWAYHDYTTKLSVSIVYALIASVALNFFYQPGHIYSSG
                     ITGLAQILTTLSKSLIGFEVPVSTTLYLLNIPLFVLAWLKIGKKFTIFTFITVTLTSL
                     CIHILPETVLSTDPIICALFGGAVMGLGIGLALRNGLSSGGLDIVSITIRKKTGKSIG
                     SISIYFNALIVFVAGILFGWQYMFYSTLSIFVSGKVTDVVYTKQKKMQVMIVTKHPEK
                     VVNEIQNCMRRGITIINEAEGAYKHDKQTVLMTIVTRYELPLLREAMKASDPQAFVSI
                     AENVQILGRFYEDGI"
     gene            38720..40603
                     /locus_tag="ACG8KR_000235"
     CDS             38720..40603
                     /locus_tag="ACG8KR_000235"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFQ0199643.1"
                     /translation="MEKQKRLKNSRYITGFDGLRSLAVVGVIFYHLLPTSMKGGYLGV
                     PIFFVVSGYLITDLLRQEWEQNGKINIWQFYVRRMKRLYPALVFMLVTASAYITLFQR
                     GLLNNLRGVVVSSLLYVNNWWQINNGLSYFDRFANESPFTHIWSLAVEGQNYLVWPIL
                     FVLLMVFVRKRKWIFYTVLGTSFLSAILMMILYTPGGDPTRVYYGTDTRLFSIWLGSA
                     LAFVWPSTRLKKNIPKQAKNILNIAGGISLLALILCFFFLDDHYNFIYYGGMLLVSLL
                     CVVLVAVTAHPGASLNRWLTNPVFTWIGKRSYGIYLYQFPVMIFYEAKVTDIADHVLL
                     HTLVEITLILIISELSYRYVERPLARFDYGRTFQVMKGWFSKPIISKSKPWVVPGTLL
                     VAIALVGFVTAPKNAVTADQKQLQAKIAESKKLAEETRNNSENTTSSVDQAILDKYEL
                     SKAQGNKAQKLELTAFGDSVMLDAATDLQELYPKVVVDGDVGRQLYASVPYIEKLKEQ
                     DLLKDTVLIGLGTNGSFSEAQFDSVMDAIGDRKVYWVNVRVPTKRWQNDVNAMLEQMT
                     KKYDNMTLIDWYSYSNDHSDWFYDDQVHPNPEGMTHYIHLVSEALLGGSSSTNSSETN
                     VTE"
     gene            complement(40678..40962)
                     /locus_tag="ACG8KR_000236"
     CDS             complement(40678..40962)
                     /locus_tag="ACG8KR_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFQ0199644.1"
                     /translation="MANEHYFYVLFCKDQTFYGGYTTDLSRRLEEHNNGTGAKYTRLQ
                     KRRPVKMIHAEVFSSRSEATKAEAAFKKLTRKQKERYLQTHSTVLLPESI"
     gene            complement(40952..41686)
                     /locus_tag="ACG8KR_000237"
     CDS             complement(40952..41686)
                     /locus_tag="ACG8KR_000237"
                     /EC_number="2.1.1.223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016430 - tRNA
                     (adenine-N6)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HFQ0199645.1"
                     /translation="MLHEDERIDQLYAEDIKIIQSSKVFSFSLDAVLLAHFSRVPKKG
                     KIVDLCAGNGAVGLFLSKRTQAAIDAIELQPRLADMARRSIALNHLNEQMTVHTIDLK
                     DSLSVVRHNSCDLVVCNPPYFKGLPTNKTNPNKHLAIARHEIHTTLEEVIDISSKLLK
                     TNGRFTMVHRPDRFLEIIQVMEDYRIAPKRIQFVYPKPGKEANILLVEGIKEGKLDGF
                     KVAPPLITYNMDGEYTAEIREMLYGK"
     gene            41815..42438
                     /locus_tag="ACG8KR_000238"
     CDS             41815..42438
                     /locus_tag="ACG8KR_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HFQ0199646.1"
                     /translation="MFYRFMRGVVRVLLALVNGNARYENKEVLPQDENYILVAPHRTW
                     WDPLYLAVAAAPKEFSFMAKEELFKNPVLRFILTRSNAFPVKRENPGPSVIKTPVKIL
                     KSTNLSLIMFPSGTRHATELKGGMALISRMSKAKIVPAVYQGPLTLGDLFKRQRVTVR
                     FGEPIDLSDIKKTDKEGMAEIERRTQAAFDKLDKEVNPDFKYEVKKK"
     gene            42630..43751
                     /locus_tag="ACG8KR_000239"
     CDS             42630..43751
                     /locus_tag="ACG8KR_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HFQ0199647.1"
                     /translation="MRFLHTADWHIGKKLQGYDLIEDQRHVLSSILDIAKEEKVEAIV
                     IAGDLYDRSVPAVDAVELYNELMVDWNLKEKIPIFAISGNHDSSTRLSAGTPWFSYSN
                     YFLRTQLAEAFEPVEFKNVQFFFLPYFEPIAARLYFEDDNIRTIKQAVAKVIDKMQEL
                     FDSEKKQVLISHFFVMGSVKSDSETKIEVGGLDSIPGELLTPFDYVALGHLHNQAALR
                     QDNARYSGSPLKFSLSEINQKKGVWIVDLTTGLELNFREIEPLHDIEEITASFKELLD
                     PAFYQSVNRENYIYIYLTDRTIIPNMMNQLRQIYPRILGIERANGRAEQTKRKRKVLE
                     MKDPQKLAEAFFSEMTEEQLTANQEKWLGETLQEIMEME"
     gene            43758..46886
                     /locus_tag="ACG8KR_000240"
     CDS             43758..46886
                     /locus_tag="ACG8KR_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFQ0199648.1"
                     /translation="MKPKKLQLKNFGPFINETVDFSRLTEAPLFLISGKTGAGKTTIF
                     DGITFALFGETSGRLRSGKEMRSLFATPDEETSVTFSFEHQQMSYEIERKPEQVLAKR
                     KGNGTKKQAAKVTLTIFDGKGKEIKQITKRTEADQLIRELMNLDAKQFSQIVLLPQGE
                     FRNFLVSSSSDKETVLRHLFGTQFFQQFNERLKEKAKQQQQKLDHLEQELHLLQKRFV
                     LVKEEEPAAGFEAVLAQWTNHQAILEKQITEEKEALTELQEKQKQLENSYYRFESLKK
                     SYEEKTILINKQKELAAQKDEIEEKKRWIGYYEFTQRLVEPIQRLQEIKVEKIELSQK
                     SSEQTKKLAMTTQNYEEWQKNEANRVRRQTKIKEAAQKLQNKQMMVPIVESLRSKKQD
                     AEQLTISLDKEELNAEKLYQNLAIYQERTRILQENLLDQEKLQEDRLAFGQLKYLDEK
                     QQEIEKQKAKDTGSLAQIREELKKATEEITKNSQLLKEQTEQAEQLKSQWAKQQIARL
                     QLFLRPGEPCLVCGSTEHPIQQLKHEQPNKEDILKVEQQLAEAESAVERTQQIIAKSE
                     AHENNLQQQLSDLEQKIMATNIQLAEQEEHLQQALKKAFPEITEQKTKTTLVVIEQSL
                     DRRKSKMANARIELEEIRQQAEKLEQQLAVYKQQQTEKNNQLQQLQGEIKSLEQQLQD
                     TDSNCLEEEIRRLEKQLEEDSNQLAVEQKAGVQLLQDLHTLRTQLDLQHEQIEKIDEK
                     IEKEQERIAKKLESQSYFSTIEEICAFAQQQAQYKENQRTVTSYHEEQLVTADRLRQL
                     ENIQLTETFPDTEEISQQLEEIRRMIEIKQEKIYGLQEQKISNQKIYDECLSIYHTSQ
                     TKLDELTQLQQLSQTFNGENPKKTSLERYVLQVYLQEVLQVANDHLQRLTKNRYQFEL
                     ADTIGSYRGKTGLEINIYDDQAGMSRSAHTLSGGESFIAALSLALALADVIQTQAGGV
                     AIDALFIDEGFGSLDEEALEMAMEALETIESEGRMIGIISHVRELKERVLQQIRIDTE
                     GSGQSKIRYQLG"
     gene            46905..50099
                     /locus_tag="ACG8KR_000241"
     CDS             46905..50099
                     /locus_tag="ACG8KR_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2 helicase associated domain-containing
                     protein"
                     /protein_id="HFQ0199649.1"
                     /translation="MKWSIPEKVIARGRQYLQEGRVLSVVPDQEENVWHAEVLGSELY
                     MVDLDGTAKEIDYCQCPYWEEHKYCKHTVAVELYLRSKNLSRIMKKDQPMLEPVKATS
                     EGEIIAKGFSRLKTQNNPQAVQPLIIEYQVETIETNQYHPELSILALYLRIGSLASPK
                     KTYIVKNIYDFLQAYTEKETYTVNKQYQFRLDKRAFQKEDQKILTHLAGSAQTHQLLG
                     TNGLQVKGKLDKRYLLLPVEQARFLLEQMNQTTRLHLQINEQNDQGIQFSDQNKPLSF
                     KVEKFGEDYLLQALNDFDFFFMHYQWGVIQGTFYSLTKYQQTVYQTWKQLIRRLEKPE
                     VVFKKKELPSLFKEIIPLLSEIGDVAIAPAVEEEVADIPVEFIFFFRKAKGKIQARVD
                     FVYGEVIYSTDPKHEVSTEQAFQVLRDTVEEKRVLDLFRMYRYQKNETGYERPLPSGE
                     ELYAFFRTELSVFRQFGEVRLGRKLRELYLDAHKHQPKIEVTETGSWLDIRFDVTGIE
                     EQEIDHVLQSLLRNDAFYTLENGQVLSFDSEEFQQTSQVLQQLRESIRTEEGTIHVPK
                     NQGLIIQNQLEKSNATFSESFQTMVQDLIHPERYQAQLPKGLNATMRDYQKQGFRWLK
                     MLGHYQFGGILADEMGLGKTLQTIAFLLSEKEERKSFSALIVAPASLIYNWQAEVRKF
                     APSLSIQVINGNKKEREELLAKDTDIRVTSYASLRQDLANYQSQKIDYLILDEAQMVK
                     NSSTKTAQALRELAVPQRFALSGTPIENNLEELWSLFATIMPGFFPTKTKFRELAAEE
                     IAQMIRPFILRRDKQTVLKDLPEKTEMNLYSALTEEQKTVYLAYLRQMREEITSMDSE
                     AFKKNRIGILAGLTRLRQICCDPRLFIEDYQGGSGKLEQVKDLLVAAKENKRRVLLFS
                     QFTGMLTILQEELAELGISTFYLRGSTKPQDRLSMVDAFNAGEKDVFLISLKAGGTGL
                     NLTGADTVILYDLWWNPAIEEQAAGRAHRIGQKNVVEVWRMISEGTIEERMDSLQQEK
                     RELFQKVIQGNEEQLTKLTEEDIRMILSVGEIEE"
     gene            complement(50245..50952)
                     /locus_tag="ACG8KR_000242"
     CDS             complement(50245..50952)
                     /locus_tag="ACG8KR_000242"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFQ0199650.1"
                     /translation="MIKSVVFDVDDTMYDQQQPFRNAVKRVVPLVSDADMHPLYIRFR
                     HHSDENFPKVMAGDWTLEYMRAHRISQSLKDLDYPHITEEDGLLFQKIYEEELDNICL
                     HEEVKKTLDFLKEKNVPLGIITNGPTDHQTKKLKQLQLNNWIPSRNMLISQATGFQKP
                     EKEIFQLAEKEFHMLPEETLYVGDNYDNDVLGAKSANWQALWFNHRERKIEQTPICDI
                     EISSFDQLLETMKTIFV"
BASE COUNT        16698 a         8928 c        10613 g        14793 t
ORIGIN      
        1 aaggcaaaaa gatgaactag aaatagttca tctttttttg tttaaatctt ttgctaagtt
       61 gaactaatag agtcttttat gaatatcgaa gttagttttt cacctaactt cgttttttat
      121 agttggttat tataaaatag taatatagct gatttggatt caattcatat ttatttataa
      181 atcaaaaata cagagaatag tatataaatg tcctttccta ttcttaaaat gatgtttgta
      241 cttacttaaa ttaggtataa ttagtatatg ttttgtgaga aatttagact tacaataagc
      301 agtgaattta taatcaacgt ataggagaca tggatgaaat gaaactaatt ggtatagatt
      361 tagacggtac tcttttgaat tctgaccaga gcattagtca aaaaaatgca agaacaatga
      421 aacatttacc ggaagactgt tttccattca tttgttctgg aagagaagta gaagacatta
      481 ataatattct caaaaaatct ggtttaagga taccagcagt agggttaaat ggttctgttg
      541 gatttgatgg agaaaaaaag atatttgagt cttccttcga taaaaatgat gtaaaaaaaa
      601 ttgattctgt cctatcgaaa tttccaacca aaatatatac aaatctcgga agttatgaat
      661 ctcagaatta taaaaataaa atgaaaaaaa tttttcacga ggtagggagt gaattttcta
      721 tcaaagagct aaactatgag cttgagtatg aaaaatctat ttgttcaatc ccttataata
      781 atattgagga aattatagaa aaggatgcta tcagcattta caaatttttt atttttattc
      841 ctaatcgtca attgaaagct aatatattta accatttgaa aaagatgcta aatgtgtctg
      901 ttactgaatc ggcacctgta aatattgaaa tcgtacctca aaatgtatct aaaggtgtgg
      961 tgtttgatcg tttggaatca atctacaatt taaagaaacc tttgagaatc gcgatcggag
     1021 atagtttaaa tgacttatct atgtttgaaa gtgcagatat tagttttgca atgggaaata
     1081 gtcatcaaga aatcatgaaa aaagctgatt atgtcactgt ttcaaatgat aaagacggag
     1141 tatctcaagc atttgaaaga attcttacca tataaactaa ttaaggtagt atagaacata
     1201 gaattctaaa tacggaaaaa tggagcataa attccagtac aaatagtact tcttcagtgt
     1261 caaacgaagc gaatataaca tagatattta ggattgtgac aaaaatcatg tcacaatctt
     1321 tttttcgaat aaacttattt tttgaagtcg gccatttatc gtaatttctt tgcataatac
     1381 gaacaaattt tcggttattg atgaaaaaaa acattgaaaa tggtatgata aaagaaaaaa
     1441 aggaggaaat ttttaatggt gaaatttatc catgcggcag atttacatat ggatcggtca
     1501 tttgaaggac tgacaactct tgataaaact gtacaggaaa aactattgaa gacgaatctg
     1561 accgttttat caaatatcat tgaacaggca ataataaata acgttgattt tgttttactt
     1621 gcaggagata attttcatca aaatcgtcct tcattaaaaa tacagaaaca tttttcagaa
     1681 cagatgaaac ggttagaaaa gaaccatata cctgtattta taatattcgg aaatcatgat
     1741 ttctatcaaa aagagcggta ttggttctcc tttcctaaaa atgtccattt atttacatcc
     1801 gaaacggtag agacaaaaaa aataacgata aaaagtggag aaactgtctc tctcagtgga
     1861 tttagttatc gtcaaccgtg gattcaaaaa gacaaagtga tggaatttcc ttctagagaa
     1921 ctcacagatt accatattgg gttgtaccac ggggaacctg gcgtctctca aaaaggaaac
     1981 tatgctcctt tccaaccctc aaagatgcaa gaaaagggat acgattattg ggctttaggg
     2041 catatccatg tgccaacggt cttaaatgaa aagcaaacaa ttgtttatcc aggtgcgcct
     2101 caaggtcata ctaaaaagga acaagcttct acatctattc tactagttga attaagtaaa
     2161 ggaagttgcc aaatacatcc gattaaagta gcagaggtct attggaagac aaaggaaata
     2221 tcattgagga aagcaagaac gacaaaagag gtcattgttc acattcgtca gcagctttca
     2281 aaagtagagg atggatttag tttaattgag ataaaattaa aagattatga ccatctcaat
     2341 acagatgttt tggaacggat acaatcagga gagctattag actatttgtt agaggaattc
     2401 tcagatagga taaatgattt ctttatttgg cgtatttctc tcatagagaa tactttttat
     2461 acgaagaccc cacttgctgc ttctgctaaa ttgatggaac agttatttca atactatcag
     2521 acacctcaag atttccaaca aattttaaat gaagtatata gtcatcaaga agcggctaga
     2581 atattgagcg aactgccgga atatcaagaa gaaacacttg aaaaagccaa gcagctgctt
     2641 aatcaagatt ttttatttga ggaggatcag gaatgaagat tttaaggatc gagattgctg
     2701 cttttggaaa atggcgtcaa aaaagttttg acttttattc aggcaatcag ctgatctatg
     2761 ggggaaacga agcaggaaaa tcaacgattt atcaatttat tcaagcaata ttatttggtt
     2821 tcccttcaaa aggccgtaaa aaaaaggatt atacaccaaa agacggctca gcctatggcg
     2881 gcaaaatatg gcttaaacat cctgtatatg gtgaatttgc tgtcgagcgc tacaagcagc
     2941 aaaatcgcgg aaaatcgaag gtgtggctgg gggatcaagt gggaagtgat gagttactag
     3001 aaaaactgat cgcacctttg acgaaagagt tattccagca ggtatttact ttccagcaag
     3061 agcagttgat ggaactagat catttgagag aaaaagagct acatgatgcg ctgatttctt
     3121 taggcatcac gggcagttct caagtattcc aaaagcggca agaatattat cagaaagcac
     3181 aacaattatt caagaaaaaa ggccaaaaat tactaatcaa tcaaaaatta aatgaatgga
     3241 aagagctgca agaaaaaatt catcaaaaac aagaacagga agcttatttt ggcaagttga
     3301 tccaacaaga acaggaatac gagtcgcaac agcagcagtt attggcacaa ttaaaagagg
     3361 cgaacaaaca attttcatta gccagacagc aagaacttaa tttttcttta tatgaagaat
     3421 ggcaatctct caaaaaaaac agcactacag gtcttcctga tgagcaactt cttttagatc
     3481 tggagtcttt ctccaaacgg tatcagcaaa caaatgagcg tttggaagaa ttagaagcaa
     3541 cactggaaaa gcatagcggg atggatcagc agtcagccag atattatttt tatttagagc
     3601 aggagcagga actaaaagat ctccagcaac ttcaatttac agcggaacaa ttaaacgaag
     3661 aatggcaaag attacaaatt gagactagag aaacaaattc agaattgaag attttggaac
     3721 aaaggtggca ttggcagaaa aatccgttac cggaagaatt tttatatgaa gaagaatgga
     3781 agcatcttct ggctgaagaa agaaggattg ctgatctaga aagccagatt tctgtgcgtc
     3841 tccaaatagc taaagaacag caagagatga ttgaagcaga agtgaatgac ttggaagaga
     3901 cctatccagc attattacaa aatcagtcag agatagcacc agtctcttca tctaaaccgt
     3961 atatgctgat ggctggcgga gcaataatca ttgcaggtat atttgtacca atgcccttga
     4021 aagtgatcct tttactaatt ggtgcaggca tgattggcta tttcttctac aaaaggcaac
     4081 caaaaattaa aactaaaaat gatttctctg agataaaagg aatgtggcaa gaaaaactag
     4141 gacaacttga tctagcctta gctcaattag ctgaagcaga agaaaaacaa aagagaactg
     4201 gagaagaaaa acagattctt cagcaagaag tatatcgttt tgcggatcat cggcacttag
     4261 gaaagatgga tacgctcaaa ctcatgaaag aatatggaca ggaagtcata gattatcaga
     4321 aattatatgg cgtatttgag cggaaacata aaagagaaca ggaaatacaa gaaaaactac
     4381 gacaaatcga ccaaaaattt gagtttgtta aagaatggtt accgatacaa gataagacaa
     4441 tttcagaaaa aatggtattg ctttcccgtt ttatacaaga gatggaacag atgaaatttg
     4501 ccagaagcta tcagcaaaat actttattga agcaagaaat caatcaattg aaaaagaaac
     4561 aaaaagtatg gatacaagaa tacagcgaac aattgactca agcaggctta tcttatcctt
     4621 cggaagtacc tgtcttttta caaaaagcca aacaagccga acagcagatg aaaagataca
     4681 atgaattatc ggaaatgctt actccactat ttcctgaaga gctgacaaaa gagaaattat
     4741 ctgtaagact gcgccaactg aaagaaaagc aatcccaact ccagcaagaa gaaaaagaag
     4801 caagtgaaaa tcggcaacga ctccaacttc aagtagaaga gttgcaaaga gacggcacat
     4861 tggacgaact ttatcaacaa gctagccggc aaaaagcaaa attacaggaa ttactggagg
     4921 aatggggagc acttgaaatc gccgggacat tcttaggtga tctttcaaca gagatgagtg
     4981 aaagacaatt accccagttg ttaagagcag caggtgatta tttaggtatt ttaacaaaag
     5041 gaaattatca gaagatatta ctgaaaaatg atacactgac tttaacagac ggatatacag
     5101 aatttcccat ctatgagtta tctacaggta cgaaagatca gctgattatg gcgatgagat
     5161 ttgcttacct ctcattagaa gctaaccgtt cgatatgccc gatcatcatt gatgatggct
     5221 ggcttcatta tgatcatcag cgaaagtatc aattagctga acttttagct aaatttggag
     5281 aaaagtatca aataatttgt ttttcttctg ataaagaaat ggtaagctat taccatgaga
     5341 tgaaccaaat cgtaaaacaa ttagaaggag caaataatga aaaaaattcg tgatttagta
     5401 atcgatgagc agtttgaaca atttgtcctg atcaaaagtg cagatgtaag aatcgctaaa
     5461 aacggaaaaa aatttattgc ttttactttc caagatactt caggtactat cgatggtaaa
     5521 tactgggatg cttcggagga ggaaatcaat aaatttacag ccggcacagt cgtttttcta
     5581 aacggtaaaa gagaggttta tcaaggaaac cctcaagtaa agattcttca tatgcgctta
     5641 acaagggtag atgagccagg agagcctagt ttatacatgg aaagagcccc tttgaagaaa
     5701 gaagaaatgg aagaagaaat caatcaattg ttgtttgaga tcaccaatgc gcattggaat
     5761 cgtattgttc gctatctact taataaatat caaaaacaat tttttgagtt tccggcagca
     5821 aaaagaaacc atcatgcatt tgccagtgga ttagcttatc atactttgac aatgcttcac
     5881 ttagggaaat caatcgccga tgaatatcct gagctgaatc gagcattgtt gtatgctggt
     5941 atcattttgc atgatttagg aaaggtgatt gaactttccg gtcctatgtc gacagaatat
     6001 acgcttgcag gaaatttgat cggtcatctg gtattgattg acgaagaaat aactaaagcc
     6061 tgtcttgcgt tgaaaatcag cgaagctgac gaagatgttc tcgtattacg tcatatggtt
     6121 ttagcacacc atggcttatt agaatatggc tcacctgtac gtccaagaat aatggaagca
     6181 gaaatattac atcaaatcga caatatcgat gcttcgatgc agatgatgtt gacttcgatt
     6241 cgtcaaacag aaccaggaaa atacaccgat cggattttcg gtatggacaa ccgaagtttc
     6301 tatgtaccaa aagatttata gcaaaaattt atttttttca ttcaaaacaa aaaaagcgag
     6361 acataagtct tcgctttttt tgttttcttt tattttgatg aatcagttgt ttcttcagtt
     6421 gaagattctt ttgttgaatc agttgatttt gtgtcagaac tctttgttga agctgtagtt
     6481 tcacttgagt ctttcgtact tgatgaagtt gttgtgaatg cactcaacac attttcaaag
     6541 gcatcatctt tgatttttac atttgcatct tttaattctt caccgattac tttagttgtg
     6601 aatgcattgt cacttagttt agtttcagta gcgatatctt ttagttgatc tttgtattta
     6661 tccatgtcat tgcctttatt ttggtttttc accattttga caacgtagta ttctgtcgca
     6721 taagaagtag gatttgttgt agtaataaca tcggagatct caccgtcttt caatttgaaa
     6781 gctgcttctt ttacttctgc aggaatagtt gttgtcgttg agtcgaattt aactttaccg
     6841 ccatcttctt ttgtctctgt atcagttgat ttgtctttgg ctaatttaga gaaatcatct
     6901 ccatcatccg ctgatttttt cacatctttt gcttcatctt cacttgataa tttgatgatt
     6961 tgtgcttcta cttctggatg gaaagatttc caagcagttt tcaaatcgtc atctgtaata
     7021 tctacgtgag ctttcaagcc agcttcaaaa gctaaattgt tacgaatata ttctttataa
     7081 gtatctttcg tatatccagc agcttctaat tgtgattcaa aagtatcacc taatgatttc
     7141 gcttgtttgt cgtattctgc atccacttgc ttatcggtta ctttatcgcc atatttgtta
     7201 ttgaatactt tataaatgat catccgttgt actaaggatt ggttcgatga ttcaagtttt
     7261 gcttcatcat agaaatcaga tacagtgatc gttccgcctt tcatcgttgc gatatcttta
     7321 ttagtatcgc ctgaacaagc tgctagagtt aaaacagcca acgcactagt agctgccaaa
     7381 ataattgatt tttttttcat taaaatttgc actcctgtaa ttagttatta tttttatata
     7441 ttaactatac catatcccat ttcctttaaa aacgttgtcg tggtagtttc aaacaaaatt
     7501 caaaatttca tcacattcct aggaaatacg caaacttttc tcacttatat agttaaatct
     7561 gttgagaaaa taatcattat tatatttgtt caggatactc tggcagatca gcttttagtt
     7621 tttctacttg ttcattgatt tgtgtgatac gtggttcggc ttggaattta aaatcttcga
     7681 tatctttttt gattgcttct tggaaagcag gaatcacaga ttcagctgtt tcttttgttt
     7741 cgatcaatgc gcttttaaaa tgattcaggc tgttatttaa atcatttgtc agagttgttg
     7801 cttcttccaa ttcttcgatc agtttctttc ttgtttcatt cccgctcctg ggcgccaata
     7861 acagacctcc gacagtgcca gcagcagctc caaataacag tcctttgaaa aagtttttaa
     7921 tcatcgcagg acacctgctt tacgattgtt tcttgaatag cttttagttc ttccgatcca
     7981 taactatctt gatgattgac aaagtgaata gaaaaatcat cctctttgct gtagcgaggg
     8041 actaaatgga tatgtgaatg aaaaacagat tgataggcta gttcacggtt gttattcaag
     8101 atattaagac ctttcatttc tggaaaagct ttttccaatg cttgagcaat cttaggcaca
     8161 cgcgaaaaaa cctctcctgc aagttcggga tcatattcaa aaatatctgc tacatgacgt
     8221 ttaggcacaa ctaatgtgtg tcctttcgtt gcttgagaga tgtctaaaaa agcatagacc
     8281 ttgtcgtctt catatatttt atagcttggg atttcttgat tgatgatttt gcaaaaaata
     8341 cagttttcca tataaaacat cctttctata aagaataaat acttcagctt aactttacca
     8401 taagttaaag ctattgtaag gaaaaaaaca aaataccgtc attttcttcg tggtataata
     8461 aataaaaata aatggcggag gaaaaagaat gagtttaatt atcgatcatg tcacaggggg
     8521 gtacggacat ctccctgtgt taaaagatat cagtttcgat gtacagcctg gagagatagt
     8581 agggttgatt ggactgaacg gagcagggaa aagtacgacc atcaagcaca ttatcggtct
     8641 gctggaagct tcaaaaggaa cgatagaaat caatcatcta tccttaaaga aaaatacgga
     8701 agaatatcgc aaacaaattg ggtatattcc agagactcct tctttatatg aagaattgac
     8761 attgagagaa catatcgaag tcactgcgat ggcctacgat atacctatgg atatagcaat
     8821 gaaaagagca gaaaagttgt tggagacttt tcggttatct aatcgtcttg actggttccc
     8881 agctaatttt tcaaagggaa tgaaacagaa agtgatgatc ttatgcgcct ttttgatcga
     8941 gccttcttta tatatcatcg acgaaccatt tttaggcttg gatccactag ctatcaatgc
     9001 attgcttgaa ttgatggtag aaatgaaaaa agaaggagcc ggtattttga tgtctacgca
     9061 tattttagct actgcagaaa aatattgtga tcgttttgtg gtcttacata acggagagat
     9121 acgagcagac ggttctcttg agatgttgag aaaagaattt cagctgccgg attcttcttt
     9181 ggacgaaatc tatattgctt taacaaagga gcaggcacat gagtagtttt tttcaaaaac
     9241 gtttagcaag acatcaaaag aaaatgatga aatatatgcg ttatgtattg aacgatcatt
     9301 ttgttttagt ttgtcttttc ttgattggcg gtgtgggatt atattattct aattggttga
     9361 aaaccctgca gccaccgttt gcaattggag ggctcataat ctctgtattt tggatgctct
     9421 gtttattcgt gggtaagatg gctacttttg cagaggcagc agatattgtt tttttacttc
     9481 ctaaagaaaa agaaatgcaa acctatttgg aacgagcttt tcgctattct tgcatttttc
     9541 cgttgatctt tttgatattg gcatgcggat tggccatgcc attagtggtg gttagtacgg
     9601 agcaatcctt ttcgatgttt ttcgtctatc tgatcatatt atggagtttg aaattcagtc
     9661 atctgcaaat caagagagca gaattgtttc gactagatca acaagttatt cgttattgga
     9721 aattaggctg gtttttttct tctctgatct ttttgtttct atcattttat tggctaatat
     9781 gggcaggaat agcaggtgca ttagtacagg caagtctata ttactggttt ttgtggaagc
     9841 aacaagaaaa aggattggat tgggagaaaa tggtgcatgt agaagaacat aggttgcatc
     9901 agatctatca atttatcaat ttattcacag atgttcctga aattacggca aagatcaaaa
     9961 gaagacgcta tttggatcct atattgactc atattaaaag agaatcaaaa aacacttatc
    10021 tttacttgtt tgcaagaaga ttggcaagag gttctgattt tagtggtctt tatttccgat
    10081 tagtaattat aggtgggtta ttgattgccg gagtagtcga cttttatttt agtctgatta
    10141 tcggtttctt atttttatat ttgatcggtt tccaactgtt acctttagcg aaccagttcc
    10201 gctatatggt tctcgttcag ctttatccaa tccataagaa tcagaagaag caggcgattc
    10261 agtatctact cacatgggta ttaattatcg cgtcggttat ttttggtttg attgctgcag
    10321 tcgtattaga tgggcagcag cgatgggttc cgctagcggc ttatttgatt gttactatgc
    10381 tctttgttaa actatatgta ccaactcgtt tgaagaagat gtcagactga aaacaaagct
    10441 gtgtagggta aaaatgagaa tgaaaaatag agcgttgaca gtaagaatgc tgttcgttct
    10501 attttttttg agaaaagctg gttttttgaa tggcaattaa atgaaaaaga acgtatatgt
    10561 gcttgacgtg tagtgttcag gcgactagaa taataagtac tgcaaaacaa agagtaagag
    10621 gacgggagaa tgcatgatgg agtttcagtt agaccaagag tggcggttac gtcccatcaa
    10681 aggcgacaca ggaaaaacat acataggcat gcgtgatgag gacaaagtct ttattaaacg
    10741 aaacacgact ccgatgttgg ctgctctttc aagggaagga attacaccaa aacttgtttg
    10801 gacaaaacga acggggaatg gcgatacttt gacagcacaa gagtggctgg aaggtcgctt
    10861 gctgactcct aatgagattg gtcaaagaaa cgacgtgatt gatgttttgt accatttaca
    10921 tcattcaaat tctcttaaaa ccatgctgac ccgtatcggt ggtcgtgtca tgacgccgga
    10981 gaagatgctg caaaaatatg aaaaagaatt accggaagaa ctccagaaaa atcaatttct
    11041 gcaaattgtc catcgttttc ttcgtgagca gatcccgtca atagataagg aaaacttatc
    11101 agtagtacac ggagatgtaa accatcgtaa ttggttagta tgtaaaaatt atttatatct
    11161 agtcgattgg gattcaatca tgtttgcaga ccctgcggtg gatattgcaa cgattttagg
    11221 aaattatgtg ccactgtcta gctggaatca gtggctgatc agttatggca tacgcccgac
    11281 tgacgaaatg cttgaaaaat ttcattggta tgcggccatg aatttgctcc aagaaatcac
    11341 cagatattgc aaacgtgaag atcatcgacg gatgaacgaa gaaattttac aattaaaacg
    11401 aattttcagt ggctgaggtt tggcccaaac tctgcggaga tttggccaga cctttttaat
    11461 tagaggaaag gaatggagat aaataatgcg tgtccgaaat cgtccgggag cagcggaaat
    11521 gttagcggct cacccgaatt ttgtaattag tgatccaaca ttgtggaagg gaaaatggaa
    11581 tgaattgttt gaaaacgatc atccaatcca cattgagatt ggtatgggga aaggccagtt
    11641 catcactgga atggcaaaag cccatcctga aatcaattat attggagtag aaatgcaggt
    11701 cagtgttgtt tcgattgcac tagacaagtt gatcgaacaa ccgcttccta acctgaaact
    11761 tctacatgtg gatggatcag cgttgacaga gtatttcgct gacagcgaag tagatcaaat
    11821 ctatctgaat ttttccgatc catggccaaa aaaacgtcat gaaaaacggc gattgacata
    11881 taagactttt cttgctgtag atgaacagat tcttcgtcca aatggagaaa tccatttcaa
    11941 aacagataat caaggattgt ttgagtattc tctggcaagt ttttctcaat atggcatgat
    12001 cctcaaacag gtatggttag atttacatca aagtcaattt gaaggcaata ttatgacgga
    12061 gtatgaagaa aaattttctt caaaaggtca acgtatttat cgagtagaag ctcaatttca
    12121 agataagacc aaaaagaaaa gtctgtgaaa ctaactttct gctttcataa aaacattaat
    12181 atccataaat aaatcccaga aaatgaacat tacagatgta atgtattttc tgggatttat
    12241 ttgatttaaa atgtatttat ttcatcggtt tttccacaag gtggaatcaa ttggaagcga
    12301 gaaaatgaaa ataatccttt gtcggtgatc ttcaattcgg gtatgacaga taatgttaag
    12361 aaagaaagtg tcaaaaaagg atcaaaggtt ttagctgctc ctagttgatg tgctttagtt
    12421 attaattcat tcaactgttc atagacgatc gaaaaaggtt cctgtgtcat gattccgcct
    12481 ataggaagag aaacacaagc tatttcctgc tgtttgttga caacggtcat cccgccacct
    12541 ttttgaatca aaagattagc agcatacagc atatcctcgt cgtttgtccc aacgacaact
    12601 agattatgcg agtcatgggc aatcgttgtc gctatcgctc cttgtttcag ctgaaatcct
    12661 ttcactatgc ctaatccaat gtttcccgtt tgatggtgac gctcgatcac cgcaatcttt
    12721 aattggtctt tttcgattga aggaatgaat cgttcgcctt gtcggtccac tttttctacc
    12781 aaatctttag tcactagact attcgggata atttcgatca catgggcttg tgaagaagaa
    12841 agaagcagct ctaacgaatg tggatttaat ggttgagcgt ttagttttgg cagacaacac
    12901 gtatatgaat ttgaatgatc agcaaataaa tggtcttgga gttgcccaga ttgaacaact
    12961 aatgttccct tatagaaaac ttcatgaatt ggcagcttgg taagatcgtc tgttaagaag
    13021 aaatcagctt gatacccagg ggcaatcgca cctagtgttt tcaaatcaaa acattctgct
    13081 gcattcaatg ttgccatctg aatggcttgt aatggatcga ttccaagtgc aatagcttga
    13141 cggacgatat gatcgattga tccttctttt actaaatcat tgatcagttt atcatcggtg
    13201 acaaagaagc atcttcttga attttcaggt gtgatcgccg gtagtagcga tgccagatct
    13261 tttgcaacgg tcccttctcg aaccattaaa tacataccaa gatccaaacg ttctttagct
    13321 tcttttgcag ttgtagcttc atgatccgtc cgaatacctg ctgcaggata aacatttaac
    13381 tcttccatgc cgataccagc tgcatgcccg tcaatttttt tcttatgctg gtgcatcaat
    13441 tggatctttt ctaggatgtc tgtttcatta tttgctactg cttgatagtt catgacttca
    13501 gctaagccta aaacttcggg tttttgtaaa aaaggggcta atttttttgc atttaaattt
    13561 gcaccattgg tctcaaaagg ggttacaggt acacaagaag gtagcataaa aaagatattc
    13621 atgagtgttt gtcttgcatc ttctatcata aactcaagac ctgtactccc agcaatgttt
    13681 cctatttcat ggggatctgt cactacagta gtaactccat gaagcaagga tacttttgaa
    13741 aattcgcttg gtgataaaag tgagctttcg atatgcacat ggccatcgat gaatcctggc
    13801 actagatatt gcccattagc attatgtacg ctttttcctt cgtattcacc gattcctacg
    13861 atcattcctt cgcagattgc tacatctttt tccatgattt ctttcgtaaa gacgttgacg
    13921 atcttagcat gcttgatcac taggtcagca ggcttttttt tacttgctac ttcgatatat
    13981 ttccttaatg ttgtttccaa tggagtgcct cccaagataa atttttataa gtataacata
    14041 aattcggttt ttgccaatat ttttttgtaa attcatttta tttgaaacaa aaagatcaaa
    14101 aagacgtatg ttttttgtgg aggggagaat aatggaaata aaacgattat cggatcaaat
    14161 gataagtgaa agttttgcca aacatgccca agacgtatat atattgccag cttcagaagt
    14221 attttacatc tattttcgct atggaaaaaa acgaacattc tggaagaaag tctcttcggc
    14281 agatggacag caactgatct caagatttaa gtatttggga cagatggata tcggagaaaa
    14341 aagaaaagct cagttaggtg catttactta ttctttagaa aaacaggaag ttcgtctgcg
    14401 gttgtcttct gtaggagatt accttcaacg agagagttta gtgattcggc tattatacgg
    14461 tacttttgaa acaccccatc gttgctttag ttctcgagat tttgcaacta ttttacagaa
    14521 aaccgtcaga cgtggattat accttttcag cggaccagtc ggatcaggca agacttctct
    14581 aatgtatcgg ttggctttgg aagaaaaatt gcaagtgatt actatcgaag atcctgtaga
    14641 gatagaagaa ccgttttttc tgcagctaca gatcaatccg aaaatagcac agacttatga
    14701 tacacttttg agactcgcat tacggcatcg tccagacttg ctaatcattg gcgagataag
    14761 agatgaatca actgcacatg ctgcggtaag agcagcacta acagggcacc gggtttttgc
    14821 aaccgtacat gcacgcaatc ttgaaggtac attggtcaga atccatgaac tgacaaagaa
    14881 aaaagaagag ctatctgaat gtttagctgg tgtcatttat caagaacttt tggaagatgc
    14941 caatcaccat ccttctgttt tatggagtta tcaatttatg gaggaagaat cagtaaaaaa
    15001 atcatgggag gaaagttatg aagaagctca ggaaaatatt tggcagtcag aactattcta
    15061 aaggattaac aagaagccag caaaaccagt ttgcacaaac catgggagaa ctgcttttat
    15121 ctggttttag tctgcaagaa tccctttatt tattactcac gatccgttcg tttccacctg
    15181 ttattttggc aaatatccaa catcagctaa aggaagggca agcgtttagt gaggtacttt
    15241 cgcaaatagg atatggagca gagcaactgg tacagatcga gttagctgaa cgtcatggga
    15301 atctgccgac aactttgttg gaaattgcca aacagtttcg gcttgttgaa ggatttcgca
    15361 atgatttgaa aaagctgctt gcatatccga tgctattgct cacctttttg ctaggtatct
    15421 tgtttagtat gcggttgttc gttttgcctc agcttattgg ttcaggaatg gtcatgcaag
    15481 agcattgggg tattcagctg atacaagtct ttcattggta tgttctacta ttcatcctag
    15541 cggttactct attggcttta ctagtaaggt atcgtctgat gaagttgtct cctgttgatc
    15601 agagtgaatg gataagcaga caattattga ttggaccgat atattccttg tatcaatcgg
    15661 cttatttgtc actagagatt ggaaagcttt ttcatgaagg tttcgaatta aggcaaatca
    15721 tagtctgctt aaaggaaacg aaacaagaaa gtctgattca gcatcttgcc tctaagttga
    15781 ttcaaggatt ggaggcaggt atgtcattgg ctgagcagtt ccagaattat cgttttctat
    15841 cagaagaatt cagtaaaatt gttttacagg gagaggcaaa gggaagttta ggtaaagaac
    15901 tgatttttta cagcgaatgg acaagaaaac agcttttcaa acgtcttcat cactggatgc
    15961 attttattca gccaatcatt tttttgggcg tagcggcttt gattttatta gtttatgccg
    16021 ctgttttatt acctgtttac ggaagcatag aggaggtttt gccatgaaaa agagaaaaat
    16081 atcttattat agcggtttta cattgatcga aatgctaatc gtcttactga tcatcagtgt
    16141 attagtgttg ttgttcgtcc ctaatttgtc gcgttatagg aatcatgtag accaagaaag
    16201 tcgtgaagcg atcatccagt tggtagacac acaaaaagag ctctatgcgc ttcaaaataa
    16261 tggaagagtt cctactgtgg aggaattgtt gaatgaaggc tatatcaaac gagaacatgc
    16321 agaaatctat cagcggcctt gaaggtaagg ttttagcatc aggatatacg ttgtttgaat
    16381 cactcttact gattttatta cttacaacga tcctttcttt tccagttctc gcattttctg
    16441 catggaagaa tgaattggca gtctatcaat ttttcggcca atttgaaaaa cgaatctatt
    16501 ctacgcaaaa aatcgctatt gtgaatcaga tccaaacagg cttttattgg aatagtgaag
    16561 agaatcaaat catttttcat gttccgaatc cagataaaat aaattgggaa gtattagata
    16621 ttcctgatga gattaccttg aaacgccatg cttctattac ttttgctgct agaaccggga
    16681 atgaaagttc actaaaagct tatcaatttt attgggaaga aaaagagcgg acgattacgt
    16741 atcaattcca gatagggagt ggtcgctata caaaaagaat tgaataaaac aggatttgtt
    16801 ttgttggagt cccttacagc gcttatgatc gcttctgtct gtgtctactt agtgagcttt
    16861 ggccagctgt ttttgcttca ggcagaaaag aaaagtgggg aggaactgct atttattcga
    16921 atgatttatg aagatatcaa gacgcagcgg atgtatcaca aaagaccaga gcgaagggaa
    16981 gaaatgatcc catacgatat ccaaattgtc gataataatg gagtaatcga aaaagtgttt
    17041 gtcaatatgg agaaagaagc gatcagtatt gaaaaaacaa gtaattccag catttacgct
    17101 gattgaatgt ctaattggac tgatgatatt gagcagtcta ttgctcacat tcaatctttt
    17161 gattcagcaa accatccaag taagtgaatt tttgcagcgt aaagatcaaa aagagtggct
    17221 aatattttta gcacaattag aagaagaact caagagcagt cacaatgtct ctgtgaaaaa
    17281 tcagcaactg cactacacga tcgaggataa acagtatatc atcgaaagat atcaggcaat
    17341 gatacgtaaa agaagaactt ccggcggaca tcaacctatg ttaacaagta tcactgagct
    17401 tcaattattg gaaaaagaac acacaatttc tttttatgta cactttgaaa atggagaaga
    17461 tggatatgcc atatggacaa aaaacgatca gtagaaaatg ttataagggc tctattttat
    17521 tttctgtctt gcttttattt cttttatgta cgtttttatt gatggcagca ttagaaagct
    17581 accgtttgtc aatggatctt aatatacgaa tcaaaaatta ttatatagca aaaataatga
    17641 agcagatggc tttatcggaa cagtctacgc ttgccgaaag ttcggaagga gttttttact
    17701 acacaactgg atctgtaaca tatcagtggg tacaacagtc cctattctta gaggtggaag
    17761 tctcaccgtt tatctttcgg ttcacagaag aagtaaaaaa tgatacagat actagtacag
    17821 aatgatggaa tgttcgtgaa aacgccctct tttctgttat aacactgttt ttaataaaaa
    17881 aatgtttgcc gtttcacaaa taaaaaacag tgaaaacgat tgaaattttg tcaattgttc
    17941 gatagaatta aacaggtgaa aaaattgtta taatatatct agaaatgagg tgcaccatgt
    18001 taccagaaaa aatggaacaa gggtttaatc aaaacctgga agctattcag cttttacaga
    18061 atgcattggg aacttccttt ttagaagcct atgtcgaaaa tgcagaaaat ttaatagatg
    18121 attatcaagt acgtgtggtg gatggagtgc cgacaaaaga gacaacgcaa cggatcacag
    18181 ctctttatga agagttgaag aaactttctt tcgagccaga agaatggcgc agactgtctc
    18241 aattactttt attgaagggc agccagacag aacatttgca ggcgaaccat caactcactc
    18301 ctgatagtat cggtttttta tttgtttttc ttattgaaca actatacaca aataaaaaag
    18361 caccggttaa gattctggat attgcggcag gaatgggcaa cttgttatta acagttcttt
    18421 taaacctatc gaatgcgggg taccaaacag aaggaattgg tgtggatatt gatgatacat
    18481 tgttggctgt cgcagcttct acaagtgatt tgacacaagc aaatgttcaa tactttcatc
    18541 aagacgggtt gcaggaatta ttaatagatc ctgtcgattt tgcaatcagc gatttgccaa
    18601 tcggttatta tccgaatgac gaaaaagcaa aagagttttt aacaagtacc gaagaaggcc
    18661 atagttatgc acatcattta ttgctagagc aatcaatgaa atatgtgaag ccggatggtt
    18721 ttggtttgtt tttgatgccg agtggatttt tagaaacgga tcaaagtgaa gaaatcaaaa
    18781 aatggtttaa agaagaaggt tatcttcaag gaatgattca attgccagat gaattgttcc
    18841 gtaataaaca atcgcaaaaa agtattttga tcttacagaa aaaaggacca caagcacaac
    18901 aagtaaaaga agtcttgctt gtgaaacttg cttcattaaa agaaccagag aaggtgacgg
    18961 aatttttcaa cgagtttaaa aactggaaat catctgctct gtaatcattc aacgagaagg
    19021 agagtcatat gtctaaaaca attgcaatca atgctgggag ttcaagtttg aaatggcaat
    19081 tatatcaaat gccagaagaa accgtagttg cgaaagggat cgtcgaacgt atcggtttac
    19141 aagattctat ttttaccatc aagtacggcg ataatcaaaa attcgaacaa atcatggata
    19201 tcgaaaacca cgaagtagca gtaaaaatgc tgttagatca gttgattgaa ctgcaaattt
    19261 taagctctta tgatgaaatc accggagtag ggcaccgtgt tgttcacgga ggagaaactt
    19321 atggggattc agtagtgatc gatgagaaag taatggaccg cattgctgaa ttagcagaat
    19381 ttgctccttt gcataatcct gcaaacttaa tggggatcaa agcgtttaaa aagattttac
    19441 ctgacatttt gagtgtggct gtattcgata catctttcca ctcaacaatg ccaaagcaaa
    19501 attatttata tagcttaccg gtgtcttatt atgaagattt caaagtaaga aaatatggct
    19561 tccatggcac aagtcatcgt tatgtgtcgg aacgtgcggc aaaattattg aacaaaccga
    19621 tcgaagaact aaaaatcatc acttgccact taggaaatgg tgtatcgatc acagcagtcg
    19681 atggaggtaa atccatggat acttctatgg gattcacacc attagctggg gtgacaatgg
    19741 gtacacgttc aggtgatatc gatccagctg ttctcccata tttaatggaa aaattagaga
    19801 ttgatattga tgaaatgatc aatgttttga acaagaaatc tggtctgcta ggcctaacag
    19861 gattgtctag tgatatgcgt gatcttgaaa agaactatga aaaagaacat attcgtttag
    19921 cttatgacat tttcgtggac cgcatccgaa aatacatcgg cggttatgtg acaacgatga
    19981 atggtgttga tgcaattgtc tttactgctg gtatcggcga gaatgacgga catgtacgta
    20041 gtgaaatcat caaaggtatg tcttggtttg gttgtgaaat cgatccagcg ttgaatgaat
    20101 tgcgtggtga agaagtagat atctctacaa aagaatcaaa agtaagagta ttagtgatcc
    20161 caacagatga ggaactaatg attgcccgtg atgtggagcg tctgcgcggc taagccaaag
    20221 cattcgttag gataaaagat gaaacgcatg caagagggaa ttcttgcatg tgtttttttt
    20281 gcggagaaga ccgcaaaatg gtaatttaac ttcaatttcc cttctttttt acaggatctt
    20341 cttagggaat tttgcttgga aaagtggtca aaagggaaaa gactggcatt ttatttattg
    20401 attttcagga aaatcccaat attttatttt cattcatctt tataagggac attacatatg
    20461 taaaaagaaa aaacattttt agatcagcct cgtgggctgt cgactttatt cttcgcttta
    20521 tgtttagcta gaaaaccgat ccgaaacatt cattgatcga ataataaaaa cttgattttc
    20581 cacctgtact ttgcctgaat cgtgaaagca caggtatgaa aatcaagttt tttgttgcga
    20641 aaaaacgtcc tagtcaatca gttggccgag cggctggcgg ctgatctgct cctgatcaat
    20701 aacgagtgct tcttggaagt gttgctgcaa aaagtagcgg tcatggctga ctgctaggag
    20761 cgttccttga tagtcttgca gcatttcttc aatttcttct cgagcatagc tgtcgaggtg
    20821 attggttggt tcatccaaaa tcaggacgtt gattttttct aaaaagagtt tcagcaacgc
    20881 caaacggatt ttttcaccgc cggataaatc tttgatccgt ttacccacat cgctttgata
    20941 aaagccactt ttggctaatt gccgtcgagc gacttcttcg tttccagtca atgtgatggc
    21001 gtaagccagc aaacggctat cgggatcggc aaactgcagc tgctgcggca aataaccgat
    21061 ttgcaagcta ctacctaatt tgatggtgcc accgtaaggc ttttcctcac cgagtaataa
    21121 tttgagtaag gttgattttc ctgcaccatt gtcgccgatg atggctaaat gatctccacg
    21181 atagactgca aaactgctgt ctttgaatag tacttgatca gcgtactgct ggtgcaagtt
    21241 ttgaatgatg aaaacttctt tgccagattt tccaccatta gacaagcttt gcagccgatt
    21301 tttcggggga ggtggcggtt tgacgagtgt cagtttcgcc aggcggcgtt ccagttcttt
    21361 ggcttttttg aagaaggact cattgtctcc ttcatgtgcc cactgacgaa aacgacgaat
    21421 catgactttc aaccgctgga cttctttttg ctgcagttcg taattttttg tcagctcagc
    21481 caagcgagct tctttcaaga ctcgaaaacg gctgtagttt ccgggatact caatcaatgc
    21541 accttcttcc agttcccatg tttttgtggt gacttgatcg agaaattgcc gatcatgaga
    21601 aacgaccaga accgattttt ttgtattctg taaatagttt tccaaccagc gaatactgtt
    21661 aagatctaaa tgattggtcg gttcatcgag taaaagtaaa tcggcatcgg ctgctaaaat
    21721 tttggctaaa gcgactttaa ctcgttcgcc tccgctaagt tctgttaacc gtgcttcctg
    21781 acggtgtgga atccctaagc cttgcaagat cgctgcaatc cggtcttcta atacgtaacc
    21841 gcctagttct tcaaagcgat gctgcagctc accgtaacgg gccagacact tctctaagtt
    21901 tgtatctggt tctgtcatca atgcttcgta gcgactcatt tgctgtttga ggtcctgtat
    21961 ctcagcggca gaatggagca agtagtggtt gacggtcgct tcggaaactg taagttcttg
    22021 ttcgaccgtc ccgatcttta agcctttttt gcgactgata acaccgctgt cgacaccttc
    22081 tctgccggtc aagatttgta aaaaggttga tttgccagtt ccgttgatac cgactaaggc
    22141 aattttttca ccagcttcaa tttgacagga taaatcctca aataatggat tcccgccgat
    22201 tgttttttta atagaaattg cttgtatgat catgatctct ctccttgaga ccactcaaaa
    22261 aggccatggt tatccatggc ctgtgttatt taaactcaga ttatcatgag aaaaatacct
    22321 tgagatggcc attgatttta cgtgaattca actgttttgg acaaacttgt cccgtcaaca
    22381 gcaactgtaa aaccaattgc atggctaaat gcgatatggt atcgttggta attttcatta
    22441 cttgttattt tagtcatgcg catcatagta ttttccctcc ttcgtgtagt tgtcttttcc
    22501 agtgtacctg aaagtgtcag aaagttcaac tagcgctgac atttacttaa tatttatcga
    22561 tgtgtaattc tgtcagagag ctttttttat ctgactgagg gtaatgtctc gtggaccaca
    22621 tcattgtatt ctgagctaaa ccagcgttca ggcgaaatca tatggtgttt gataaaaaac
    22681 tttgtctaca tgacttcctt ttgccatttg cttaataccg ttgcattaaa cagctcttct
    22741 ctttggaaat ctcaccaaca tcgcgttcag gagtatcgtt gtggttgcaa tggcaagcgg
    22801 tagcctttca tcaattcttg aatgatttac cgaatgtagc acttgccttc tcgcggtacc
    22861 cgaacccgaa ataaaatttt agtttggcga taaaatcggt atccgccatc gatacttcat
    22921 attttttcgt atcagattca tcggaaactt ccacattgac gaaccaataa gctttggcat
    22981 gcttaggtcg ttttaaacca caccagggcg actaatcgcc ctggtgttca ctttttttta
    23041 ttttacttga actcattgac tgttttccct ttattttaaa cattataaag accgatgagt
    23101 gcttgccgcg tcttttgttt ttatcacggt tttttgtgca aaaaaacctg ccgatagttg
    23161 gcaggtcgtc tacgtgttaa gctttggtgt ggtctttagc tggcaattgg ttttctaaat
    23221 cagttcggac aacaagcaca tcgcaagctg cgtttctaat aacatattca gaaacagaac
    23281 caataaataa acgttctaca gcattcaatc ctgtggctcc taacataatc aaatctactt
    23341 gattatcttc tgggatttct ctggcaataa tttgttttgg tgagccatat tctacgacag
    23401 aggttacatt attcaagcca gctttcttcg cattgctttc ataatctgcc aatgtttgct
    23461 ttgccatttc agtagcttgt tctgcaagca ttccatcaaa ggatgaaacg gtttggaaag
    23521 aacgtgtgtc aatgacatgt gctaatagaa gttctccgtt atttcggata gctacattga
    23581 ccgccttttt aaaagctaat tcagcttcat cagaaccatc tacggcaacc atgatttttt
    23641 tgtattgttg taacataagc attcactcct caagtcaatc gtattgtact cttttattgt
    23701 agaacacttt agggaaaaag agaattattt actgctttgg ttcagcaatt ttttttgcaa
    23761 atcggatact gaaggtggca gaaacaataa ttacgatcag taaaaagacc aaggcaataa
    23821 agcgttgatt gaaaaaagct gttaaaatac ccattacgcc taaaacagca tatgcagctc
    23881 cgtaaaattg taggaatcct tgatttttat catttttgtt gatcaataca aaaaagatat
    23941 cctgtttatt aaataaataa tagctaacga ataataatac taaagtggcg ataatcatta
    24001 aaatcctaat catgaacaaa tcctccaaaa taagcaacaa atagaaaaag caatttgcca
    24061 agagcaaatt gctttattct ttaaaagatg gtaatctccg ttaatgccgt tgtttgtcct
    24121 gtagtattga tcccgcgaat tcaagcaggt gggtcccatg gtcgtagctt cgaactacca
    24181 aatgataagg catgttacca gcagtgacac aagacgggtc ccaagatatc aataaaaatg
    24241 ttcggtcaac acacaaagag gacaattcga acatcacaga tttcccacct ctatagtagc
    24301 atatgaattt aaaaagagca atgagagagc tcgcagaaac agaaccgttt tcttgtctag
    24361 ttttttggag gatgcttttt ccactctttt atacggtaat actgttgacc cagagcctgt
    24421 tcatatttcc ctgtatcttt tggctgataa tactgagcat tcttcagttt atctggtaaa
    24481 tattgctgat tgacccaagc ctgatcaaaa tgatgaggat actgataacc cacgccacgg
    24541 ttgagtgatt tcgcaccttt gtaatggcta tctctcaaat gatcaggaac atctccagct
    24601 ttgccctctc gaatatcagc aagtgctgca tctaatgcca tgtaggcaga attggatttt
    24661 ggcgacaagc aaagatcgac gacgacgtct gctagtggga tacgtgcctc aggcaatccc
    24721 aatttttcag cagctaatac agcgttcact gtacgagccg cagcagcagg attacctaaa
    24781 cctatatctt cataaccgat aaccatcaga cgccggcaaa tacttgccag atcacctgcc
    24841 tcaactaaac gggcgagata atgaagtgca gcatccacat cacttccacg aatcgatttt
    24901 tgaaaagccg aaataacgtc ataatgagca tctccatttt tgtcatgggt aagagctttt
    24961 ctttggacac attcttcaat gatcgacagt gtaagatgaa tccttccttc tttatctgaa
    25021 ggagtcgata gtgtagccag ttccagccca ttcaatgcac tccgcaaatc gccgttcgtt
    25081 gcacgcgaaa ggtgaagaag agcatcttcg tctaactgga ttgcttgttg tccaaggccg
    25141 cgttctttat cccttaaagc atgttcgact gccaactgga tgtcttgttc tgttaatggt
    25201 ttgacttcaa agatctgtgt acggctgcgg atcgctggat tgatggtaat ataaggattc
    25261 tcagtagtcg ctccgatcaa gatgattcgg ccactttcta aatgaggaag cagaaaatcc
    25321 tgttttgttt tatctaatcg gtgtacttca tccaataaga ggatcactgt accactcatt
    25381 tttgcttctt ccgctacaac ttgtagatcc ttttttgaat ctgtagcggc gtttagcatg
    25441 cgaaatgcat aattagtaga gccggcgatc gcacttgcga tactcgtttt tccagtacca
    25501 ggaggtccat atagaatcat ggaagagagc atgcgagcat cgaccatgcg gcgtatgatt
    25561 tttccttccc cgactagatg ctgctgacca acgacttcat cgatcgttcg aggtctcatg
    25621 cgataagcca atggctgttg cataggattt tcctccttct tgtttttacg tcccttaatt
    25681 atatcatatt tagcaagatg cctagaaaga acagccttct gttgttttgt tttcatagct
    25741 tatccaacta atattgtgta tgataaagta ggtgagaaaa atgacggata aacaaaagat
    25801 agatgaattt ttttggaata aaacgacgcc agaagtggca cgggatttat tggggatgta
    25861 tttggagcat gacacaccag aaggaagatt agctggatat attgtagacg cggaagcata
    25921 cctaggtcca gaagacgagg ccgcccatag ctatggttta agaagaacac cgcgtgtgcg
    25981 ggcaatgtat gaaaaaccag gtacgatcta cctttacacg atgcatactc atcggatttt
    26041 gaacatcatt actcagccag aagggattcc tcaaggagta atgatacgtg caatcgaacc
    26101 cgccgcaatg attgatcaaa tgagtaaaaa tcgtggcgga aaaactggtc cagatatcag
    26161 caacggtccg ggaaaattag tggaagcatt agcaattcct caagaacttt atggtcagtc
    26221 tatagcagat agttcgttac gcttagtttt tgagaaaaaa aagacaccaa aaaagatcaa
    26281 tgctttgcct agaatcggta tcccaaacaa aggtgtatgg acagaaaagc cgttgcgttt
    26341 tgttgtgtcg ggaaatcctt atatctcttt acaaagaaaa aaccaaatcg agaaagattg
    26401 gggttggagg aaagaaaatg aaaaagaagg atctattaac atatttagat gaaataattg
    26461 aaaaacaagt gacagactat gatgtagctt tagattggga tacaaaaaat catacgatcg
    26521 agatcgttgt tcgcttgttt gcagaaaata aagcacaatt tgagattgat gatgcagaag
    26581 ggatcgtttc gcaagaacca attatcgaat ttgaagacgg cgtacttctt tttaatcctc
    26641 agaaatctgt ttttgatgaa cgcaattatc tagcagtaat cccttatgaa ggaaaaaaag
    26701 ggttggcaaa atcagtagct gattctttag tggaatatct aaatgaagta ttagctcaag
    26761 ggcaaagcga tttgcttgat tttttggatg aagacgatga cgaagctgta ttcgagcttc
    26821 actgggataa tcagcaacta gcagaattag tagaagtaaa acagcaaaat gaagcagata
    26881 cttatttacc gtacccaagt tattaaggag tgacagatat gaaatggaat gaagtaaaag
    26941 tagaaactga aagtgaagca gtcgaagcag ttgctaatat tttgatggag gcaggggcga
    27001 gtggtgtcgc gattgaagat tctttagatg ttgaacattt tcagtcggat caatatggtg
    27061 agattttaga taaaaatgat tttacatcgc tgaaagaagg cgcactagtc atggcttatt
    27121 ttccggaaac gactttctta ccggaaatct tgccttttat caaagatcgt atcacacaat
    27181 tacctgagtt tggtctagct attggaaaaa ataaagtaac agttagtgaa gtagaggaaa
    27241 gcaactgggc aacagcctgg aagaaatact atcatccggt gcgtattacg cgaatgctga
    27301 cggttgcgcc aagctgggaa aactaccaga cagaagatcc tctagaaaaa attatcacac
    27361 tagatcctgg tatggcattt ggcacaggca ctcatcccac gacttccttg acattgacag
    27421 cactagaaac agtcttgcgt ggtggtgaaa cattgctaga tgtgggaact gggtcgggcg
    27481 tgctaagtat tgcagcaaaa tatcttggtg caaaagatgt ctatgccttt gatttagacg
    27541 aagtagccgt tcgttcggcg aaagaaaata tggatatgaa tgaaatagcc aaagatgtcc
    27601 atgtatcagc taacgatctt ttgacaggaa tcgagattga aagtgatgta atcgttgcga
    27661 acattttagc agatatcatc ttattgatga tcccagatgc ttggcgttta ctgaagcaaa
    27721 caggcacgct gatcgtttca ggaatcattg aagagaagaa gcaaatggtt ctggatgcca
    27781 tgttagaaca agggtttatc gtggatcaaa ttttccagca aaaagactgg tatgcaatta
    27841 ttttgaaaaa accagaggtg gaatagatgc agcggtattt tttaaaagaa tcatatgaat
    27901 caaaagacta ttttgtttta gaaggggaaa actatcacca cattgtacgg gttatgagga
    27961 tggaacccga ggatgaagtt tttttggcat tcaatgatca aatagccatt gttgcagaaa
    28021 tcacggagat tactgaagag gctgtttatc tgaaagaaat taaaaaagaa gaaaatcaaa
    28081 aggaactgcc aagttcgatt accatagcga gtggctatcc taaaggggaa aagctggatt
    28141 ggatcgttca gaaatccact gaattaggag cgcaagcttt tcttggtttc ccggcatcag
    28201 cctctgtcgt taaatgggat gcaaaaaaac gtatgaaacg acaacaacgg cttgaaaaga
    28261 ttgctcaaga ggcagcggaa caatcccatc ggcagatcgt gccatcagta gagttattgg
    28321 aaacgaaaca agatttattt gagcggctag atgattatga cgtggtcttg gttgcttatg
    28381 aggaatcagc aaagcaaggc gaaaaatcaa atgcagccaa aattcttagt gaaacaaaac
    28441 cagatagtcg aatcttagct gtctttggac cagaaggtgg attatcccgc gatgaagtcg
    28501 attattttgt agaacacaaa gggatacttt gtggattagg accaagaatc cttcgtacag
    28561 aaacagcacc tctttattta ttaagtgctg ctagctatca atgggaatta atgaatcctt
    28621 ctttcagcta acgttcttca ttaataaaag agtatcgaca gaaagaattg aaaaaatagt
    28681 caacaatttg tataatagaa acaattattt atacaatgga tacgaaaaat cgtttgaaaa
    28741 agcttttaaa aagcgaacat ttccgtaaaa aaacgtataa ctgcacggat tttgggacaa
    28801 ttatcaaaca gatatttgtc ccgattcccg ttttaccgaa aaaagaaagg gggatcgatt
    28861 cctgtgacaa aagaagaaat tttaacggga cccggtgtca taaagctcgt tagtcaatac
    28921 atgggtcccg aacatgtagc atttgttgag aaagcatgcg aatacgcgac tgctgcacat
    28981 gacggacagt ttagaaaatc aggcgaaccc tacattattc atcctatcca agtcgcaggt
    29041 atattagcag atttaaaaat ggatccccat acagtggcta caggcttctt acatgatgtt
    29101 gttgaagata cagaaatcac tttagaagat ctgagagaag aatttggcga tgacgttgct
    29161 atgttagtgg acggcgtaac caaattaggg aaaataaaat ataaatccca cgaagagcag
    29221 ctagcagaaa accaccgaaa gatgctgcta gcaatggctc aagatttacg agttatcatg
    29281 gtcaaattag ctgatagact acataacatg cgtacgttga agcacctgcg agaagataag
    29341 caaagaagga tcgctcagga aactttagaa atctatgcgc ctcttgcaca tcgtctaggg
    29401 atcagccgga tcaaatggga attagaagat acagcacttc gttatctaaa tccaaaacag
    29461 tattaccgta tcgtccactt gatgcagacg aagagagaag aacgtgaaaa atacgtaagt
    29521 ggtactgttg aagatattcg aatagcgaca gaagagctgg ggatttttgc agaaatctat
    29581 ggacggccaa aacacattta ttcaatctat cgtaaaatga aagatcagaa aaagcagttc
    29641 aacgaaattt atgacttgct agctattcga gtgatcgtag attcaatcaa ggattgttat
    29701 gctgtattag gagcaatcca cacaaaatgg aaaccaatgc ccggcagatt taaggattat
    29761 attgccatgc caaaagctaa tatgtaccaa tctttgcata ctaccgtgat cggtccggca
    29821 ggaaatccgg tagaaattca aatccgaaca caggaaatgc atgaaatcgc tgaattcggg
    29881 gttgctgcac actgggccta taaagaagga aaaaatgaaa aagtagaacc agatggtatg
    29941 acgaaacaat taagctggtt ccatgagata ctcgaacttc aagacgaaag ctatgatgct
    30001 tctgaattta tggaaggcgt aaaaggagat atctttagtg ataaagtcta cgtcttcaca
    30061 ccaaaaggag acgttactga gttaccaaaa ggatccggac cattggactt tgcatacagt
    30121 atccatacag atatcggtaa caaaaccact ggtgcaaaag taaatggcaa aatggtgcag
    30181 cttgattaca aattgaaaaa cggagatatc attgagatca tgacttctcc aaattcattt
    30241 ggcccaagtc gcgactggtt gaaattagtt gctactagca aagcaagaaa taagatcaaa
    30301 cgtttcttca aagcccaaga tcgagaagaa aatgtgatca aaggccacga atccgtggtc
    30361 aaatgtatta cagatctagg atttacgcct aaagatattt tgacgaagaa caaactgcaa
    30421 gaagcactcg atcgttttaa ttatcaaaca gaagatgatc tctatgcagc tgtagggtat
    30481 ggagaagtta gccccttgac gatggccaat cgtctgactg aaaaagaacg taaagaacaa
    30541 aaaatcgagc agcaaaagca agaagcagaa gaaatcatga atcagccgaa aaaagaacct
    30601 gacaaaatga aagtacgtca tgaaggtggc gttgtcattc aaggggtaga aaacttacta
    30661 attcgtatca gtcgctgctg taatccgatt cctggtgatg atatcgttgg ttatatcact
    30721 aaaggcagag ggatatccat tcatcgtcga gattgtccga atgttcagcc tgacaaacca
    30781 aatgtagcag aacgtttgat tgaagtcgaa tgggaagata catcgaatac acgaaaagag
    30841 tatgatgcag atttggaaat ttacggctat aatcgttcag gcttattgaa tgatgtactt
    30901 caaacagtca atgcgctaac gaaaaatctc aacagcgttg aagcacggac gaataaagat
    30961 aaaatggcga cgatccattt gacggttggt atccagaatt tatcccacct aaagagtatc
    31021 gtggataaaa tcaaagcagt acctgatgtc tacagtgtac gccggacgaa tggataggag
    31081 gacagatatg agagcagttg ttcaacgggt aagtaaagct gctgtgtcag tcgatcagca
    31141 agaaattgga aaaattggct acggcctact tgttttatta ggcattcatg aaaccgatac
    31201 gcaaaaagat gtggactatc tggtaaaaaa aattgcacaa atgcgtattt ttgaagatga
    31261 acaaggaaaa atgaatatta gtgtagaaga tgtgggagga caaattttaa gtatctctca
    31321 attcacactg tttgcggata cgaaaaaagg aaatcgacca agctttgtgg aagccgcacg
    31381 accagaaaca gctattcctt tatacgaatc attcaatgaa ggaatcagaa gtcgcggcat
    31441 cacagtcgct accggacaat tcggagcaga tatgtcggtt tctctcgtca atgatggacc
    31501 tgtaacgatc attattgatt ctaaaaacaa ataaagtaat ctggttttca ctagtaaaaa
    31561 ctatgaaaca cgtcgcttaa gttagactga taaactctaa cttaagtggc ttttttgtct
    31621 gcaaaaagcg cttcttttgt cagcagtcgg aaaaaaagtt aagcttaaat tatacaagtt
    31681 attttttata ggaggttgct gaagatgaaa gaagtaccag tagtagatat caaaatcaca
    31741 gatttaaaaa aattgtatca aaaaggatac aacatgttgg aggagttacg ccatgaagcg
    31801 gacgctcccg tcgtcaaagc aaagattttc aataaagaag ctatcacgat ctacggatca
    31861 tcagcagcaa aagtatttta tgatccaagg aatttcaaac gaaaaggtgc tatgccaaaa
    31921 ttagtattga agacactttt tggtcaaggt ggagtacaga ctttggatgg agctgctcat
    31981 caccatcgga aaaatatttt tatggatcta atgacaccgg aaagaatgga agattatcat
    32041 cgtatcttag ataagaatct gacacaagca cttgaagcac agcatggtca atttgaacta
    32101 tttgatttaa gtaagatggt tttctttaca tctatttgcg agtgggcagg tatcaattta
    32161 tcagcgattt ctaaagatga ggtcgaaaaa ttagcagaat atcaaatctc tatgatcagc
    32221 ggaacgttta cttctccgat tgatcacata aaaggtgtcg aaaatcgcaa aaaatcagaa
    32281 aaatgggcgc aaggcttgat tgaagaagct cgacaaaatc ctgtggctgg aaaagaaaat
    32341 gtggcattat atgcttttgc caatgccaca gatttagatg gtcagctttt acctctggaa
    32401 gtggcagctg ttgaattatt gaacatcatc cgtccaacgg ttgctctaac tgtgtgggcg
    32461 gcattgatgg gacatgcact tttcagtaga ccagatttgt atcagcaatt aaaaaatgac
    32521 ttttcgacgt tacaagatcc gtttattcaa gaaatgagac gctattatcc tttcttccca
    32581 atgctacctg cgatttcgct gaaagaagta gaagtagatg gttatcgtat accagaaggc
    32641 agttgggtta ttttagattt atatggaaca gaccatgatg aacgaacagt tgaagcgcca
    32701 gattccttta tgatcaaacg gtatgtaggg aaagcaaaag atatttccta taaagaagaa
    32761 tatgagatga ttgcacaagg cgggggaaat tttcgacaaa tgcatcgctg tgcaggagaa
    32821 tggatcactc ttcatagtct acgtgtgttt tccgatcagc tagtaaataa gtttgagttc
    32881 tctgtacctg aacaagattg gacgattcca ttcaatcagt ttccgactta tccgaatagc
    32941 cgagcgctac tttacaagaa ttagattagc ataaacttat cgaaaaaaag gaagatcaaa
    33001 cagtgctgat cttccttttt tcatattact ataaattgat aaggtcaatt gatcaaaatc
    33061 cacgaaatgc ttcatcaatg gtttgataat cttcattgga taaatgcaaa tctaaagctt
    33121 ggacattgtc agatacttgc tctggacgct tagctccagg tataactaca gacactcgtg
    33181 gattcttcat gtaccaagct aaaacaagtt gcgcaggagt ggcttggtat cttttagcaa
    33241 tggggcgaag tacatcgaca gcggttaaaa tttctctgaa acgttctcct tggaaatctg
    33301 gatttctttt tcgaggatct ccttcaccaa attgtttttc ctcaccaagt tcatattttc
    33361 ctgtcagcaa accggaagca agaggaaaat agggaacaaa actgattttg tttttttcaa
    33421 gataagggaa taattctttt tcagcctgcc gatggatcaa actgtattta tcttctacta
    33481 catcgacata tccatctgca ttcgcttctt tcaattgttc taacgtgaag tttgacacac
    33541 cgactgctcg gatttttcct gcttctttta attcgtgcaa tgtagcaact gattcatttt
    33601 ttggtgtgct ttcatccgga aagtggatat aaaaaatgtc gatgtaatca gtttgcagac
    33661 gttttagtgc atcttccacc gattgtttga gaaactcagg agaattatca aatgttcgcc
    33721 ctttatttgg tacatgagca gcttttgtag cgatgacgac acgtgagcga tcatattctt
    33781 tttccttcag tacctcgcca atcagttctt cggaacggcc gtttccgtaa gcataagcgg
    33841 tatcgatcaa attgatcccg ctattcaaag ctgtacgaac tacttgtttt cctgtctcat
    33901 cttccaaacc gtcaaaaaga ttatgtcctc caacggcatt tgctcctaat ccaagctgtt
    33961 ctgcgtagac ttgtgaatgt ccgattctta tttctgccat agctgattcc tcctttttgt
    34021 ctagtctaaa acaaaatacc aaaaagctga actaaaacgt ttttgtgagg ggaaatagtc
    34081 agatatctct tgactttgag actggttttt agtaggttat aagtaatatc gaatgtcaat
    34141 gaaagagcga gtaagttttt gaaactttac aagagaggat catcgaggct gaaaatgatc
    34201 taaagtttca ttagcctaaa gacactctgg agatggacga gtgaaaagcc gtcccgcgta
    34261 cgagcgttat tcgtaagagg aaagagaaat ctttcaaaat taggtggtac cgtgtatgaa
    34321 tttcattcac ccttgttcat gatgaacaag ggtgattttt ttatttacat agctaagaaa
    34381 gggagagaaa actatgagtt tccaaagacc aaaaggaaca aatgatattt tgccaggcga
    34441 atcagaaaaa tggcagtttg tcgaagaaac agcacgttta ttattcagag attatcagta
    34501 taatgaaatg cgtacaccga tttttgaaca ctatgaagtg atatcccgaa gtgtagggga
    34561 tacaacggat atcgtttcaa aagagatgta tgatttttat gacaaagggg aacgccatgt
    34621 gacgcttcga ccagaaggta cagcaccaat cgttcgttct tttgttgagc ataaattgtt
    34681 cggcccagag tatgcgaaac cgttcaaaac tttttatatg ggaccaatgt ttcgctacga
    34741 acgccctcaa aaaggacggt tacgtcaatt ccatcaaatc ggagtagaag catttggatc
    34801 agaaaaccct gcaactgatg tggagagcat ggtgatggca cttgatttct tcaaacagtt
    34861 ggggatcacg caaatccggc tagtcatcaa ttcattaggt gataaagaaa cgcgtcaagc
    34921 ctatcgtcaa gcattgatcg actatctttt accttttgaa tcagagctga gtgaagactc
    34981 taaacgccgc cttcatgaaa atccattacg cgtattggac agtaaagaca aacgagatca
    35041 aaaatttgtg gcagaagctc catctatctt agattatcta agcgagcctg ctcaaaaaca
    35101 ttttgatacc gttgtttcca tgctggatgc attggctgtt ccttatgaaa tcgacagtaa
    35161 tatggttcgc ggactagatt attacacaca tacgattttt gaaatcatga gtgacgcacc
    35221 aaaaatgggg gcacaagcaa cgatttgcgc aggcggacgt tatgacaatc tagtggaaga
    35281 actaggtggt ccttcaacac ctggcttcgg atttgcaatg ggtatcgagc gattactctt
    35341 gactatggag gcagaggaag tagttatccc tgcatttaat gagttggatg cttacgtggt
    35401 cgcactcgga gacgaaacaa atatcgaagc attgaaagtg gtccaagcga ttcgaaactt
    35461 tggtttttca gctgaccgcg acttcatgaa ccgtaaagca aaagcgcaat ttaagacagc
    35521 agataaagcg aatgccaaat tagtattggt gatcggtgaa gatgagcttg ctagtcaaac
    35581 gattaatgtc aaagcaatgg caagccgaaa agaaaaaagt tttccattag aagagatata
    35641 tgaacatttt gatgaagtct atgatgaaat gactttattc gagtaggagg aaaaaaataa
    35701 atggcacaaa gaacgatcta ttgcggatta gtttccgaac aacaattaga acaaacagtt
    35761 acattaaaag gatgggtaca aaaaagacga gaccttgggg gagtaatctt tatcgaccta
    35821 cgtgatcgtg aaggaatcgt tcaaatcgtc tttaatcctg aaatcaacaa agaagcatgg
    35881 gaaatcgcgg ataaatgtcg cagtgaatac gtgatcgaag tgactgggga agttagaaga
    35941 cgcggagaaa atgcagtaaa tcctaaaatg gccactggtg aattcgaagt gattgcaagt
    36001 gaaatcgtga ttttgaatca agcaaaaacc ccgccatttc aaattgaaga tgatcaagca
    36061 atcagtgacg aaatacgttt gaaatatcgt tatttggatc tgcgtcgtcc aaaaatgact
    36121 aaaaacatca aattaagaaa tgcgacaaca aaagcgattc gtcattattt agatgataat
    36181 gactttctcg atatcgaaac accttaccta gggaaatcta caccagaagg cgcaagagac
    36241 tacctagttc cttcacgtgt ccatgctgga catttctatg cgttgccgca atctcctcaa
    36301 ttgtttaaac aattgctgat gaatgctgga ttcgaccgtt attatcaaat cgttcgttgt
    36361 ttccgcgatg aagatttacg tggagaccgt cagcctgaat ttacgcaagt agatattgaa
    36421 acaacgttct taacagcaga agaaatccaa acttacacag agggattgat tgcgaaggta
    36481 atgaaagaag ttcgcggtat cgaagtcaca ttgccatttc ctcgtatgac ctatgatgaa
    36541 gcaatggctc gctacggcag cgacaaaccg gatacacgtt ttgatatgga attgatcgat
    36601 ctaagcgata cagtcaaaga agtagaattc aaagtcttcc aaatggctct tgaaaacggc
    36661 ggtgtcgtga aagcattgaa tgctaaaggc gctgcagatc gctactcacg aaaagacatg
    36721 gatcagttag gtcaatatgt aggacaattc ggagctaagg ggcttgcatg gctgaaagta
    36781 gaagaagacg gattgaaagg tccaattgct aaattcatgg gagaagcaac agaagcaatc
    36841 attaaagcaa cagatgcaaa accaggtgac ttgctgatgt ttggcgcaga taaaagtgaa
    36901 atcgtcgctg ctgcattagg tgctatccgg acacgtttag gaaaagaatt aggactaatt
    36961 gatgaatcga aattcaattt cttatgggta actgattggc cacaatttga attttcagaa
    37021 gaagaaggtc gatatgtctc tgctcaccat ccattcacga tgccaaaaga agaagatatc
    37081 ccgttattgg ctgaagaccc tgcaaaagtc tatgcagaag cttatgatat cgtcttgaac
    37141 ggttatgaac taggcggagg ttctatccgt attcatactc gtgagcttca agaaaaagta
    37201 ctagaaactc taggctttac aaaagaacaa atggaagaac aatttggttt cttgttaaca
    37261 gctttagatt acggattccc tccacatggc ggtattgcat taggattgga ccgttttgca
    37321 atgttattag ccggagaaga taatattcgt gaagtcattg cctttcctaa aaacggaaaa
    37381 gcagcagatg tgatgagtga tgcaccaagt atggtttcgc ctttacaatt gttcgaattg
    37441 aatattgatg taacgaatat agaagaatag ccgataaaaa ggtaaaaagg atttgtcgaa
    37501 tacgcttctt ttgacaacct tgatttaatt gaaaaacact ttataatgaa gccataacat
    37561 ataaagcggg gcttttccat gaaaaaaaca ttcgaaaatt gggcttatca tgattacacg
    37621 accaagcttt cggtatctat cgtttatgcg ttgatcgcat cagtcgcatt gaatttcttt
    37681 tatcagccag gacatattta ttcaagtggt attactggtc tagctcagat tttgacgact
    37741 ttgtcaaaat cactgatagg attcgaagta ccggtatcaa caactttgta tttactgaat
    37801 atacctttgt ttgtattggc gtggttaaaa atcgggaaga agtttacaat tttcactttt
    37861 atcacagtca ctttgacctc cctttgtatc catattttac cggaaacagt cctatctacc
    37921 gatccaatca tctgtgcgtt gtttgggggg gctgtgatgg gactaggtat tgggctagcg
    37981 ttgagaaacg gcctatcatc gggcggtcta gatatcgtga gtatcacgat tcgtaaaaaa
    38041 acaggaaagt cgattggttc tatttcgatc tacttcaatg cattgatcgt ttttgttgcc
    38101 ggaattctat ttggctggca atatatgttt tacagtacgt tgtctatttt cgtcagtgga
    38161 aaagtaactg atgtcgttta tactaaacaa aagaagatgc aggtaatgat cgtaacgaaa
    38221 catccagaaa aagtagtaaa tgaaatccaa aactgtatgc gtagagggat cacgatcatc
    38281 aatgaagcag aaggtgctta taagcatgat aagcagacag tattgatgac gatcgttaca
    38341 cgatacgaac tgcctctact tagagaagca atgaaagctt ctgatccaca agcttttgta
    38401 agtattgcag aaaacgtcca gatacttggt cgtttctatg aggatggcat ttagatcgag
    38461 tttgacaaaa attcatgaaa tactgctgta gacagacgtt cgtttgtctg cagttttttt
    38521 ctaccatagt agaatcagca aagaaaaact gcttttttat ttgaaatttt cttaaagttg
    38581 aaaaaaagca tgtttatttt tttaaattta tgggatgata gaaaaaaata gtagagaaag
    38641 gacacgattc tacttgctat tttcacttaa tacccgtata ttacgaatga aaaaagttta
    38701 tgaaaagttg gaatattgaa tggaaaaaca aaaaaggtta aaaaacagcc gctacatcac
    38761 tgggtttgat gggctccgtt cattagctgt ggtgggtgtt atcttctatc accttctgcc
    38821 aactagtatg aaaggcggct atttgggcgt tccgattttt ttcgtcgtat ctggttattt
    38881 gatcacggat ctgctgcggc aggaatggga acaaaatgga aaaatcaata tttggcaatt
    38941 ttatgtcaga agaatgaaga gactctatcc ggcacttgtt tttatgcttg tgacagcttc
    39001 ggcttatatc actttgtttc agcgagggct gttaaacaat cttcgaggag ttgtggtcag
    39061 cagcttactg tatgtcaaca actggtggca aatcaataac ggtttatctt atttcgatcg
    39121 atttgctaat gagtcaccgt ttacgcatat ctggtcttta gcagtagaag gtcaaaacta
    39181 tttggtttgg ccgatcttgt ttgtattgtt gatggtattt gttcgtaaaa gaaaatggat
    39241 cttttatact gtgttaggta catccttcct ctccgccatc ttgatgatga tcttgtatac
    39301 accaggaggc gatccaaccc gtgtttatta cggtaccgac actcgtctgt tttctatatg
    39361 gttgggaagt gcgcttgcgt ttgtttggcc aagtacgcgt ttaaagaaaa acatcccgaa
    39421 acaagcgaaa aatattctga atatcgctgg aggaatctcg cttttggcac tgattctttg
    39481 cttcttcttt ttagatgatc attacaattt catctattac ggaggaatgc tcctagtgag
    39541 tctgttatgc gtagtgctag ttgcagtgac agcacacccc ggagcaagtt tgaatcgatg
    39601 gctgacaaat ccagtattta cttggattgg caaacgaagc tatggcattt atctgtatca
    39661 gtttccagtt atgatttttt atgaagcaaa ggtgactgat attgccgatc atgttttgtt
    39721 gcatacttta gtggaaatca cattgattct aatcatcagt gaactttctt atcgatatgt
    39781 ggagcgtccg ctagcacgtt ttgattatgg tcgtacattc caagtgatga aaggatggtt
    39841 cagcaaaccg atcatcagta agagcaaacc atgggttgtt ccaggcacat tacttgtagc
    39901 aattgcactc gttggtttcg ttacggcacc gaaaaacgct gtaacagccg atcaaaaaca
    39961 actgcaggca aaaatcgctg aaagtaaaaa attagcagaa gaaaccagaa acaattcaga
    40021 aaacaccact agttcggttg accaagcgat ccttgataag tacgagttgt ccaaggcaca
    40081 agggaataag gcacaaaaat tagaactaac ggcatttggt gattcagtta tgttggatgc
    40141 tgcaacggat ctacaagaac tttatccaaa agtagtggta gatggagatg tcggacgaca
    40201 attatatgcc agtgtaccgt acattgaaaa attaaaggaa caagatttgt tgaaagacac
    40261 tgttctgatc ggactaggaa cgaatggctc attctcggaa gctcagtttg atagtgtgat
    40321 ggatgcaatc ggtgatcgaa aagtatactg ggtcaatgtc cgagtaccga cgaagcgctg
    40381 gcaaaatgac gtgaatgcga tgcttgaaca gatgacgaaa aaatacgaca atatgacgct
    40441 gattgattgg tacagctaca gtaatgacca tagtgactgg ttttatgatg atcaagtcca
    40501 tccaaatcct gaagggatga cacattacat tcacttagtc agcgaagcct tgctaggagg
    40561 aagttcaagt acgaacagca gtgagacaaa cgtgacagaa tgatagctac aaaaaacttc
    40621 aggcaaaaac atttgttttt gcctgaagtt tttttatatt taccgtgttt ttaacattca
    40681 tatagattca ggaagcaaaa cggttgagtg tgtctgtaaa taacgttctt tttgttttcg
    40741 ggtgagtttt ttgaatgcag cttctgcttt tgttgcttcg cttcttgaag aaaatacttc
    40801 tgcatggatc atcttaacag gtcttctttt ttgaagacga gtatatttag caccagtacc
    40861 attgttatgt tcctctaatc gtctagaaag atcggttgta tatccaccat aaaatgtctg
    40921 atctttacag aataatacgt aaaaataatg ttcatttgcc atagagcatc tcacgaatct
    40981 ctgcagtgta ttctccatcc atattgtaag tgatcaatgg tggagcgact ttgaatccgt
    41041 ctaatttgcc ttctttgatt ccttcaacta acaaaatatt tgcctctttt ccaggttttg
    41101 ggtaaacaaa ttggatcctt ttaggtgcaa ttcgatagtc ttccatgacc tgaatgattt
    41161 ctaagaaccg atcaggacga tggaccatag taaagcgtcc atttgttttt agtagcttac
    41221 ttgaaatatc gatcacttct tctaacgtcg tatgaatctc atgacgggcg attgccaggt
    41281 gtttattagg gttcgtttta tttgttggca gccctttgaa ataaggtgga ttacagacga
    41341 ctaagtcaca agaattatgt cgaacgacag ataagctgtc tttcagatcg atggtgtgga
    41401 cagtcatttg ttcatttaaa tggttaagcg cgatactacg tcttgccatg tctgctagac
    41461 gaggttgaag ttcgatagcg tcgatagccg cctgggttcg tttacttaaa aaaagtccaa
    41521 ctgcgccatt tcctgcacaa aggtcaacaa tcttcccttt tttaggtaca cgggaaaagt
    41581 gagcaagcag cacagcatct aaagaaaaag aaaacacttt ggaactttgg ataatcttga
    41641 tatcttctgc gtataattgg tcgattcgtt cgtcttcgtg aagcatggat tccctccaaa
    41701 atgaaaaaaa tagtcgtctg tattatacta tttttattaa aaagaaccaa ggtggcttgt
    41761 caaacctcta ttttttatgc atactatgac cataagaagg gaaaggaaga gactatgttt
    41821 tatcgattta tgcgcggggt agttcgagta ctattagcct tagttaatgg aaatgcccgc
    41881 tatgagaata aagaagtttt accgcaagat gagaattaca tactagttgc tccccatcgt
    41941 acttggtggg acccattata tctggcggta gctgcagcac ccaaggaatt cagctttatg
    42001 gcaaaagaag aactgttcaa aaatccagtt ctgcgattta tattaactcg atccaatgct
    42061 tttccagtta aaagagaaaa tccaggacca agcgtcatta aaacacctgt aaagattttg
    42121 aaatcgacaa atttgagttt gatcatgttt cccagtggga caagacatgc tacagaactt
    42181 aaaggcggaa tggcattgat ttccagaatg tctaaagcga aaatcgtgcc agctgtatat
    42241 caaggtccgt tgacacttgg ggatctgttc aagcgtcaga gagtgacagt gcgtttcggc
    42301 gaaccaatcg acttatcaga tatcaagaaa acggataaag aaggtatggc ggagatcgaa
    42361 cgacgaacac aagcagcttt tgacaaattg gacaaagaag tgaaccctga tttcaaatat
    42421 gaagtcaaaa aaaaataaaa tgataagttt atgcgtgggc ggtcttttga atctactgtc
    42481 ctgttcagtt cagacttttt caatgaaaac gagggagaga cgtcaagtct gccctctttt
    42541 tgcgttccaa tatgtaaaat agaagacagg tgtcatgtaa tggatcaaag tgttataata
    42601 attagaagga cattttttga ggtgattgaa tgcgtttttt acatacagcc gattggcata
    42661 tcggaaaaaa actacaaggt tatgacttga ttgaagacca gcgtcatgta ctatcttcta
    42721 tactagatat cgcgaaagaa gaaaaagtag aggcaatcgt gattgccgga gacttatatg
    42781 ataggagtgt cccagctgtt gatgccgtag aattatacaa tgaattgatg gtcgattgga
    42841 atttaaagga aaagatcccg atttttgcta tatcaggaaa tcacgatagc agtacgagac
    42901 tttcagcggg tacgccatgg ttcagttatt cgaactattt tttgcgaaca cagctagcag
    42961 aagcatttga accagtcgag tttaaaaacg tacaattttt ctttcttcct tactttgaac
    43021 caattgcagc gagattatat tttgaagatg acaatatccg aacgatcaag caagcggtag
    43081 ccaaggtgat agataaaatg caggagctat ttgattcaga aaaaaaacaa gtattgatta
    43141 gccatttttt tgtaatggga agtgtaaaat ctgattcgga aacgaaaatc gaagtaggcg
    43201 ggttggattc gatccctggt gaactactta caccgtttga ttatgtagca ctaggacatt
    43261 tgcataatca agctgctttg agacaggata atgctcgcta tagcggttcc ccattgaaat
    43321 tctctttatc tgagatcaac caaaaaaaag gcgtatggat cgttgacctg acaacaggcc
    43381 tggagttgaa ttttagggag atcgaacccc tgcatgatat tgaagaaata acagcgagtt
    43441 tcaaagaatt gctggatcct gccttttatc agtccgtcaa tcgggagaat tacatctata
    43501 tctatctcac tgatagaacg atcatcccga atatgatgaa ccagcttcgg caaatatatc
    43561 ctcgtatttt ggggattgaa cgggcaaacg gtcgagcaga acaaacaaag cgcaaacgaa
    43621 aagtactaga aatgaaagat cctcaaaaat tagcagaagc attttttagt gaaatgacgg
    43681 aagagcagct aactgcaaac caagaaaaat ggctcggaga aacgttgcaa gaaatcatgg
    43741 aaatggaatg atcaagaatg aaaccgaaaa aattacaact taaaaatttt ggtcctttta
    43801 tcaatgagac cgtagatttt tcacgtttga cagaagcacc gctttttttg atcagcggga
    43861 agacaggagc tggcaaaacg acgatttttg atgggatcac cttcgcactt tttggtgaaa
    43921 cttccggaag acttcgctct ggaaaagaga tgcgttcttt gtttgctaca ccagacgaag
    43981 aaacaagcgt gactttttct tttgaacatc agcagatgag ctatgaaatc gaacgaaaac
    44041 cagaacaagt tttagcaaaa cgaaaaggaa acggaacaaa aaaacaggca gccaaagtaa
    44101 cgttgaccat ttttgatggg aaaggaaaag aaataaaaca aatcacaaaa cgtacagaag
    44161 cagatcagtt gatcagagaa ctgatgaatc tcgatgcgaa acagttttct caaatcgttt
    44221 tactacccca aggagaattc cgtaattttt tggtttcatc aagcagtgat aaagagactg
    44281 ttttacgtca tttattcggt actcaatttt tccaacagtt caatgagcga ttaaaagaaa
    44341 aagcaaaaca acagcaacaa aaattggatc atttagagca agagctgcat cttctgcaaa
    44401 aaaggtttgt tttagtcaaa gaagaggaac cagcagcagg gtttgaagcc gttttagccc
    44461 aatggacaaa ccatcaggca atactcgaaa aacaaatcac ggaagaaaaa gaagctctaa
    44521 cggaacttca ggaaaaacaa aagcaactag aaaattccta ctatcgtttt gaaagcttga
    44581 aaaaaagtta tgaagaaaaa acgatattaa taaacaagca aaaggaatta gctgctcaaa
    44641 aagacgaaat agaggaaaag aaacgatgga ttgggtatta tgaatttacg caacgattag
    44701 tcgaaccaat ccagcgccta caagaaataa aagttgaaaa aatagaactg tcacaaaaaa
    44761 gtagtgagca aacaaagaaa ctagcgatga ctacgcagaa ttatgaagag tggcaaaaaa
    44821 atgaagcaaa ccgcgtacgc cgacaaacaa aaatcaaaga agctgctcaa aaactgcaaa
    44881 acaaacagat gatggtgcca attgtcgaat ccttacgtag taaaaaacaa gacgcagaac
    44941 aattaacaat ttcattggat aaagaagaat tgaatgccga gaagctgtat caaaatctcg
    45001 ctatctatca ggaaagaact cgaattcttc aagaaaattt actggatcag gaaaaactgc
    45061 aagaagatcg tcttgctttt ggacagttaa aatatctaga tgaaaagcag caagaaatag
    45121 aaaaacaaaa agcaaaagac actgggtcgc tcgcacagat aagagaagaa ctcaaaaaag
    45181 caactgaaga aataacaaaa aatagtcagc tattgaaaga acagacagaa caagcagaac
    45241 agctgaaaag ccaatgggca aagcagcaaa ttgctcgtct tcagcttttt ttgcgtccag
    45301 gggagccatg tcttgtttgt ggatcaacgg aacaccccat acaacaatta aaacacgaac
    45361 agccaaataa ggaagatatc ttaaaagtag aacaacaact ggcagaagct gagtctgctg
    45421 tagaaagaac tcagcaaatc attgccaaaa gtgaagcaca tgaaaataat cttcaacaac
    45481 aattatcaga tttagaacaa aaaatcatgg caacgaatat acaattggct gaacaagaag
    45541 aacacttgca acaggctttg aaaaaggcat ttcctgagat aacagaacaa aaaacaaaaa
    45601 caactcttgt agtaatcgaa caatctttag atagacgaaa aagcaagatg gccaatgcaa
    45661 gaatagagct ggaagaaata agacaacaag cagaaaagct cgaacaacaa ttagctgtct
    45721 acaaacagca gcagacagaa aaaaataatc agctccagca actgcaagga gaaatcaaaa
    45781 gtttagaaca acaattgcaa gatacggata gtaactgttt agaagaggag atacgacgac
    45841 ttgaaaaaca gttagaagaa gactcgaatc aactagctgt tgagcagaaa gcaggagtgc
    45901 aattgttgca agatcttcat acgcttcgga cacagcttga tcttcaacat gaacaaatag
    45961 aaaaaataga tgaaaaaata gaaaaggaac aagaaagaat agctaaaaag ttagaaagcc
    46021 agtcgtattt ttcaacaatt gaagaaatct gtgcatttgc acaacagcaa gcacaatata
    46081 aagaaaacca acgtacagtt acttcttatc atgaagagca attagtgaca gctgaccggc
    46141 tgcgtcaatt agaaaatatc cagctaactg agacattccc agatacagaa gagatcagcc
    46201 aacagttaga agaaataaga cgtatgatcg aaataaagca agaaaagata tacggtttac
    46261 aagaacaaaa aattagtaat caaaaaatct acgatgaatg tctttcgatt tatcacacaa
    46321 gccagacgaa attagatgag cttacgcaat tacagcagct ttcgcaaaca ttcaacggag
    46381 aaaatcctaa gaagactagt ttggaacggt atgtgcttca ggtttatctt caagaagtgt
    46441 tacaagtagc aaatgatcat ctccaacgtt taacgaaaaa tcgttatcaa tttgagctag
    46501 cggataccat tggcagttat cgagggaaaa ccggattaga aatcaatatc tatgatgatc
    46561 aggcaggtat gtctcgaagt gcgcataccc tgtctggcgg tgagagtttt attgcagcac
    46621 tttctttagc attagctctt gccgatgtga tccagacaca agctggtggt gtagcaatcg
    46681 atgcattgtt tattgatgaa ggatttgggt cattagatga agaagctttg gaaatggcaa
    46741 tggaagcttt agaaacgatc gaaagtgaag gaagaatgat cggcatcatt agtcatgtaa
    46801 gagaattgaa agaacgagta ttacaacaaa taagaattga tacagaaggt agcggacaaa
    46861 gcaaaatacg ctatcaactt ggataaaaag ggagattggt aactatgaaa tggagtattc
    46921 cagaaaaagt cattgcacgt ggacgtcaat acttgcaaga aggccgagtg ttatcggttg
    46981 tccctgacca agaagaaaac gtgtggcatg cagaagtctt gggcagtgaa ttgtatatgg
    47041 ttgatttgga tggtacagca aaagagatag attactgcca atgtccctat tgggaggaac
    47101 ataaatattg taagcataca gttgctgtgg aactttattt acggtcgaaa aatctatctc
    47161 gtatcatgaa aaaagaccag ccaatgctag aaccggtaaa agcaacaagt gagggagaaa
    47221 tcattgcaaa aggtttttct cgtctaaaaa cacaaaacaa tcctcaagcg gtgcaacctt
    47281 tgattattga ataccaagta gaaacgattg agactaatca gtatcaccca gagctttcta
    47341 ttttggcact ttacttgcgt atcggttcat tggcatcacc gaaaaaaaca tatatcgtaa
    47401 aaaacatcta tgatttttta caagcttaca cagaaaaaga aacttacact gttaataaac
    47461 agtaccagtt tcgtctagat aaacgggcgt ttcaaaaaga agatcaaaaa atactcacgc
    47521 atcttgcagg aagtgctcag acgcatcaat tattaggtac gaacggctta caagtcaaag
    47581 gaaaactaga taaaagatat ctgcttctcc cagtagaaca agcccgcttc ctgttagagc
    47641 agatgaatca gactacacgt ttgcatctgc agataaatga acaaaacgat caaggaatac
    47701 aattttccga tcaaaacaaa cctttatcat tcaaagtgga aaaatttgga gaagattatt
    47761 tgctgcaagc attgaatgat tttgattttt tctttatgca ttatcagtgg ggagttatcc
    47821 aagggacatt ttattctctg acgaaatatc aacaaacggt ttatcaaact tggaaacaat
    47881 tgattcgccg attggagaaa ccagaagtag tgttcaagaa aaaagaatta ccgtctttgt
    47941 ttaaagagat tatccctctg ctgtcagaga tcggcgacgt agctattgcc cctgcagtag
    48001 aagaggaagt agcagatatc cccgtcgaat ttattttctt tttccgaaaa gcaaaaggaa
    48061 aaatccaagc tcgagtcgat ttcgtctatg gcgaagtcat ctattcgact gacccgaagc
    48121 atgaggtctc gacagaacag gcttttcagg tgttacgtga cacagtagaa gagaaacgag
    48181 tacttgatct attcagaatg tatcgctacc agaaaaatga aactggttat gaacgtccat
    48241 tgccaagcgg ggaagaacta tatgcctttt tccgcacaga actatctgtc ttccgtcagt
    48301 ttggtgaagt cagacttgga agaaaattgc gagagttgta tttagatgcg cataaacatc
    48361 aaccgaaaat cgaagtaacc gagaccggtt catggctcga tattcgattc gatgtgacag
    48421 ggatagagga gcaagaaatc gatcatgtcc tgcaaagctt gctgagaaat gatgcttttt
    48481 atacattaga aaatggtcag gttctttctt ttgattcaga agaatttcaa caaacaagcc
    48541 aagtgttgca acaattacgg gaatcgatac gtacagaaga aggaacgatc catgtaccta
    48601 aaaatcaagg attgatcatt caaaatcaat tggaaaaaag caatgctact tttagtgaat
    48661 cgttccaaac gatggtacaa gatctcattc atccagaaag ataccaggca cagcttccca
    48721 aaggattgaa tgcaacgatg agagattatc aaaagcaagg ttttcgatgg ttgaagatgt
    48781 taggtcatta tcagtttggc ggtattttag ccgatgaaat gggacttggg aaaacactac
    48841 agacaattgc ctttttactt tcggaaaaag aagaacggaa aagcttctct gctttgatcg
    48901 tagcgcctgc gagcttaatt tacaactggc aggcagaggt gagaaaattc gccccttctt
    48961 taagtatcca agtaatcaat gggaataaaa aagaacgtga agaattactg gctaaagaca
    49021 cagatatccg agtgacttct tatgccagct taagacagga tctagctaat tatcagtcgc
    49081 aaaaaattga ttatttgatt cttgatgagg cacaaatggt caaaaatagc agtacgaaaa
    49141 ctgcacaagc attgagagaa ttagctgtac ctcagcggtt tgcactgagt gggacaccaa
    49201 tcgagaataa tttagaagaa ttgtggtcat tgtttgcaac gattatgcca ggatttttcc
    49261 cgactaaaac aaaattcagg gaacttgccg cagaagaaat tgcgcaaatg attcgaccgt
    49321 ttattttacg gcgagataaa caaactgtgt tgaaagactt gccggaaaaa acagagatga
    49381 atctatattc tgcacttaca gaagaacaaa aaactgttta tttagcttac ttgcgtcaaa
    49441 tgcgtgaaga aatcacatca atggattctg aagcattcaa gaaaaaccgg atcgggatac
    49501 ttgctggtct gacaagactg cgacaaattt gctgcgatcc tcgtctgttt attgaagatt
    49561 accaaggcgg ttccggcaag ctcgaacaag taaaagatct actggtagct gccaaagaga
    49621 acaaacgaag agttttgctg ttttctcaat ttacagggat gctgactatc ttgcaagaag
    49681 agttagccga gctagggatc tcgacatttt atttaagagg cagtacgaaa ccacaagatc
    49741 ggttaagtat ggtagacgca tttaatgctg gcgaaaaaga tgtttttctg atttccttaa
    49801 aggctggggg aactggattg aatctcacgg gagcagatac ggtcatcttg tatgaccttt
    49861 ggtggaatcc tgccattgaa gaacaagctg ctggacgtgc acatcggatc ggacaaaaaa
    49921 atgtggtaga agtgtggcgt atgatctctg aaggaacgat tgaggaacgc atggattcac
    49981 tccagcaaga aaaacgcgaa ctgttccaaa aagtgatcca aggtaatgaa gaacagttga
    50041 cgaaactgac tgaagaagac atccgcatga ttttaagtgt aggagaaatc gaggagtaag
    50101 agactcgaaa aagcgttcag taatgggtaa gaaagccttt ctaaagaagc gattgtttat
    50161 ttgatttaaa aaatagcaag gaggctggga ttccatttta tcccagcttc cttgctatca
    50221 tttttttatt tctattttta aaggttatac aaagattgtc ttcattgtct ctaacagctg
    50281 atcaaaagaa gaaatctcga tatcgcaaat tggagtctgt tcaattttgc gttctcgatg
    50341 gttgaaccaa agcgcttgcc agttagcact ttttgcacct agaacatcat tgtcgtaatt
    50401 atcgccaacg tataaggtct cttctggaag catatgaaat tctttctcag ctagctgaaa
    50461 aatctctttt tctggttttt ggaaacctgt tgcttgagaa atgagcatat tgcgggaagg
    50521 aatccaattg ttcaattgaa gttgcttcag tttttttgtc tgatggtcag tcggcccatt
    50581 tgtgataatt cctaatggga cattcttttc cttcaagaaa tcgagggttt ttttgacctc
    50641 ctcatgcaag cagatattat caagttcttc ctcgtaaatt ttttgaaata agaggccatc
    50701 ttcttccgta atatgagggt agtctaaatc ttttagtgat tggctgatcc gatgtgctcg
    50761 catatattct agtgtccaat ctcctgccat tacttttggg aagttttcat cactatgatg
    50821 gcggaaccgg atatatagtg gatgcatatc tgcatctgaa acaagcggaa cgactctttt
    50881 gaccgcgttt cgaaatggct gctgctgatc atacatcgta tcatcaacat caaacacaac
    50941 tgatttaatc aaaattttac acgcctttcg gttttcattg attttttcac aaatccttga
    51001 ctattgtaac agaaaaaatt gtctaaaaaa gc
//
LOCUS       DAZKLD010000005        47233 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.005, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000005 DAZKLD010000000
VERSION     DAZKLD010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 47233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47233
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.005"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            218..2362
                     /locus_tag="ACG8KR_000243"
     CDS             218..2362
                     /locus_tag="ACG8KR_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFQ0199651.1"
                     /translation="MKNKKTIRLLAATWLIAPSVLSTNTVFAVDNQIADQTVTTDTTT
                     SSSATSETTDSFTEDPTGTTNSTTGSTEPTEIDPADPSENEKPAETYSLTIAPSLIGK
                     IQLTASTADSPEQQPLDITESGTVEGLLAETKVTYTITPAEGEQIASVAVNGAKEVNY
                     DAKTFTVGTNNLSITAEFKAGPDTSTEKPTDPSEEVPSTPDPETPNKPDVGNNNNNNG
                     SNNSNNNNNNSNSGNSSNQTNPSGSNNNGNTQREPNHPNNGSSNGGTNNASSRPNTAN
                     QSGIENPSSASSDFVVKSPIDAVLPTNTTNVQQAIVREAFKHLGKPYVWGAKGPNTFD
                     CSGLTYYVYMKATGHYIGGWTGEQQYAGTQIPVSQAQPGDLVFWGPSSGVTHHVGIYI
                     GNGQFIHAPQPGDKVRVTSISDFTPDFAVRVNLAGLPAASGSLTGGSSILDGLDGSFH
                     FSQNQTTDQFLKKIADDAQEIGQKEGIYASVMMAQAILESGSGNSLLSSEPNHNLFGI
                     KGSYKGSSVTFNTLEQDSSGQSYQIRAQFRKYPSYKESLEDYADLIKNGLTGNPDFYK
                     PTWKSETKDYKEATKYLEGRYATDRQYSQKLNAIIEAYDLTKYDEPKKEAATTEEANA
                     EILNMSKRFDVPIKWTTDGVPITGLEFSTIKRVNNASSIFRVASIWDLWNNFTARQIP
                     ETTTRTVKAATVPVLTLLSLDKSFWLDLPLKK"
     gene            complement(2414..3454)
                     /locus_tag="ACG8KR_000244"
     CDS             complement(2414..3454)
                     /locus_tag="ACG8KR_000244"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HFQ0199652.1"
                     /translation="MEINEQNFGENYHLITITNQNGTSLSVSDLGARVVHFQTIIDNE
                     KRELILGFDEAKEYLEKDPYIGASIGRMAGRITNGTFKLDDETFEVKTDPDTGHCLHG
                     GAPSFEAKQWHYTVINGENEASVIFYQTSPDMENGFPGTLDVEVRYTLTNDDTWRVTV
                     QGISDRKTVFNPTNHVYFNLTGDPAQSIDEHILWLNSRLFATLNKDTTPTGEVVSVSG
                     TAFDFQRPKKLGEVFASDFRQSMIVGGIDHPFLLKKQSGLLNAAELTSPDEKVSIQVK
                     TDASGIVIFTANFGEDGPEMRGNKLVNHGGITFETQELPGAERLESFGDVTLEPNQVR
                     EFVTEYKINIKK"
     gene            complement(3731..5533)
                     /locus_tag="ACG8KR_000245"
     CDS             complement(3731..5533)
                     /locus_tag="ACG8KR_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0199653.1"
                     /translation="MLWKLSLTGIKSRLRDYLVLFSGLVMASAIFYMFESMASNEAFL
                     KSNTIVAATVIIFRFGSVLLGIITFVYILYANSFLMTMRQKDYAMFMMLGAKGRKIAQ
                     MIFSETFLVGIAATLVGSAVGVGLTSIVHRLLVDQLDLNISHFTPFSIHGLLITVLFF
                     AVLFLLAAVVNAFSIVKKPILALIRADQTPTRIKQHKFLFFLEVVLGIAFLGCGYYMM
                     KHVGTYQVFGIGVALVTIVLGTYFIFHSVIIFFLSLLKKSEQISLKKLNNFTLSQLNF
                     RIREYTQMLSMVAMLFALALGALTVGLGFKNEIADLTENNSGYDLVMNNPRAEDQQKI
                     KELSPTLSASYTQKEDAGTIYYNASEFAAQPLIMIKHEDGTPPKITKEKVPAKKMNEL
                     TVQEELRSYELPEQQEKEIKVVSQSEFDQLNLPQSTLQLVQVKDFQANLKPIETLVAQ
                     NKMNNPSLQKVEYSNQKYEMYEVYNGLFSGFEFMGFFLGLAFLTMLASCLMFKILSGS
                     KSDIARYEMLEKIGTRRGLLKQSIRREVGVLFLAPGILGVVHVLFGLQMFGLFMQNPY
                     KDIWVPFTIFFVLYFIYYVLTTWLYTGIVLKKRL"
     gene            complement(5547..6299)
                     /locus_tag="ACG8KR_000246"
     CDS             complement(5547..6299)
                     /locus_tag="ACG8KR_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0199654.1"
                     /translation="MNKIVEVQNVTKVYGKNNEKKTQALSGISFDVEKGEFIGIMGAS
                     GSGKSTLLNILSTLDKPTDGHIRINQKDVTTLKGNQLADFRANEIGFIFQDFNLLESL
                     TAQENIAVPLSLQGVRPKEIKQRVQKIAERLSISHILESYPSEISGGQKQRVAAARAL
                     ITQPTILLGDEPTGALDSKSARDLLDTMDELNTKDHVSILLVTHDPFSASYCQRILFI
                     KDGGIHQEVKRGDQSRDSFYREILSILGNLEQ"
     gene            complement(6308..6670)
                     /locus_tag="ACG8KR_000247"
     CDS             complement(6308..6670)
                     /locus_tag="ACG8KR_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFQ0199655.1"
                     /translation="MKKLLIGLLVFIVVVLAGVKIADYVVMGGEEYYVQITTDGTKTV
                     SKSDSGQETVNYRYILPGYTKEGKEKELDFKGQLTRPLRKGAYLKITWNKNKGVTSYE
                     EVEKKDVPKTALEKLIKG"
     gene            complement(6846..7718)
                     /locus_tag="ACG8KR_000248"
     CDS             complement(6846..7718)
                     /locus_tag="ACG8KR_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFQ0199656.1"
                     /translation="MREKRAFYINGYAGIVGLVVLALVGLFFFYLGMWQAKVWALFLS
                     IFLWLITLLLLSSATVVSPNQAKVILFFGQYLGTIRENGFFLTIPLAQKMTVSLKVRN
                     FNSSVLKVNDLDGNPIEISAVVVFKVVDTAKALFDVAYYQDFVEIQSETAIRHIASQY
                     PYDTFNENDLTLRGNTTAVSDELQKELQERLAVAGVEVIETRLNHLAYATEIASAMLQ
                     RQQAKAILSARQTIVEGAVTITQMALEQIEDGQEINFTDDRKVQLINNLLVSIITDKG
                     TQPVINTGDMKDRK"
     gene            complement(7769..8257)
                     /locus_tag="ACG8KR_000249"
     CDS             complement(7769..8257)
                     /locus_tag="ACG8KR_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HFQ0199657.1"
                     /translation="MKVEEGQREKLKTEMERLHTYITQLSQTFYDPDKEKVMVNYPNN
                     SEGRQLEQVYHEVFKHLLTVKKELDYYSLPIIDTGILKYDGKKERFIFKSVREDLPLS
                     AGMDLEVLVEDYFTEEKHWVRTKLDYLPQAAGGTQASGWYITEDKELELEGVMARIRK
                     KN"
     gene            complement(8395..9186)
                     /locus_tag="ACG8KR_000250"
     CDS             complement(8395..9186)
                     /locus_tag="ACG8KR_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HFQ0199658.1"
                     /translation="MLTGGLLAGATYTLGAQKSLTWDNGPRLIDMVTETKKIKDANKI
                     VIHAENQTVSVIRGLDFEIKTTYDKTEKPEIKEKNGVLTVNAVKKSTGAIISLEDTSA
                     QITIVVPADHELSELTIEGNNSAIDINEVSIKKVNTKTHDGWVSFNEFTGGTITAKDE
                     LGSLRLDEANVDNLIVTGENSNFHLEDSLISKEGKVILNGGNLSLHEMENSGYTIQTS
                     GESYIEKDYQPITNTLLEQGQKKLKITAKDTSINITTDQEDNETD"
     gene            complement(9241..9849)
                     /locus_tag="ACG8KR_000251"
     CDS             complement(9241..9849)
                     /locus_tag="ACG8KR_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HFQ0199659.1"
                     /translation="MNEYLNEVVDHLQEIPEDDRFDLIQYYEEYFLDSGKTLDQIIDE
                     YGTPKQFALKLKISYFSEEEQAENELVKNPKRQIRLIWLIIIGLFASPVLIPLALGFI
                     LVIGGLLVALLALIFSIYVLCISILGVGLFMIISGISVFGQSILSGLFFIGVGLFATG
                     SAVFISPLLLKGTKGLFYVMMNFVKWMGRRFITKRDLHSAGV"
     gene            complement(9842..10159)
                     /locus_tag="ACG8KR_000252"
     CDS             complement(9842..10159)
                     /locus_tag="ACG8KR_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFQ0199660.1"
                     /translation="MHIQIPTVLLDGTVLAALSKEDMYGYALTKIVQESLSVSESTLY
                     PVLRRLKKEGYLETYDEPFEGRNRRYYSLTPMGSRHLEEIIDEWIVFRDSLDRLLEES
                     TNE"
     gene            complement(10315..11043)
                     /locus_tag="ACG8KR_000253"
     CDS             complement(10315..11043)
                     /locus_tag="ACG8KR_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HFQ0199661.1"
                     /translation="MPTGVLINVGSVLFGGLIGGLVGNKLSEHFKAQLTMVFGVCSMG
                     MGIYSIAPMKNMPAVIFALVIGTAIGLIVHLGNGINKGAALMQVPISKIFPSEKLGMT
                     HDEFISTLVTVIVLFCASGTGIYGSLDSGMTGDSTILISKSVLDFFTAAIFACNLGYV
                     VSVIAIPQFIIFYILFLLAKFIYPLTTPDMILDFKACGGFLMVATGFRMINVKMFPVA
                     DMIPAMIVIMPLSWMWTNWIMPLL"
     gene            11202..12083
                     /locus_tag="ACG8KR_000254"
     CDS             11202..12083
                     /locus_tag="ACG8KR_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFQ0199662.1"
                     /translation="MDIHKFEVFLDLAETMNFTKTADRQFTTQGNISKQILSLETELD
                     VKLFERAHRKIELTEAGSLLLPYVKNVVEQYHAMQEVLETYTKDNNLSLNILTIPTMI
                     NYKGFSKITEFLKRHPEFTVQLKEVESVELTYNDANEHSDTIHFARSFQAPPSSIEWL
                     PTETDDFVAVLPKNHPLASKKKLDLSELKKENFLLLGPTTNLYQPVLDLCREAGFEPK
                     ISYKGARIDLIINMIANDMGIAIVMEKTVKNLLKENTVILPISPTTESYLAFTRKVGE
                     HSLASDTFWEYLNQEEN"
     gene            complement(12320..13363)
                     /locus_tag="ACG8KR_000255"
     CDS             complement(12320..13363)
                     /locus_tag="ACG8KR_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFQ0199663.1"
                     /translation="MKKKLFSQLWIYFASIVFISILVTLLCFLGFIFLLSRHSISPAE
                     SAGKPTLYPLFVFAGFSMVVGTGISIFVGRRILHPISALGTNMSLVATGDFSIRMDEQ
                     QKVAEVQQLYKDFNVMVQELNSIETLRNDFVSSVSHEFKTPLATIQGYVQLLQAPNLS
                     DEERQIFLYRIIESITQLSQLTENILKLNKLENQRIQLEKKEYRLDEQIREVIVFFQP
                     KWEKEQLELDIELAAVNYTGNEEFLYQVWLNIMDNAIKYNQINGQIHIKLFETATEIV
                     LEVTDSGVGMNEETRDRMFEKFYQGDTSRQISGNGLGLSLVKKILELHDGRIDYSSIE
                     GVGTTAMIRLSKQ"
     gene            complement(13366..14034)
                     /locus_tag="ACG8KR_000256"
     CDS             complement(13366..14034)
                     /locus_tag="ACG8KR_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFQ0199664.1"
                     /translation="MNRILVVEDDPQLQLLYRSVLERAGFSVITALNGEDALKKLEAT
                     RIELIITDIMMPKMDGYDLLDSLRSTRFDTPVLMITAKADFEDKKRGFKLGADDYMTK
                     PIDVNEMILRVEALLRRSKINYSSSLIIGQTKLELDAYTVLQDQHSLILPQKEFLLLY
                     KLLSYPNKIFTRQQLMDDIWGLDTNTEERTIDVHIKRLRTRFEKTEDFQIITVRGLGY
                     KAVI"
     gene            14176..16512
                     /locus_tag="ACG8KR_000257"
     CDS             14176..16512
                     /locus_tag="ACG8KR_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HFQ0199665.1"
                     /translation="MLQLKDIKKYYKVGETTTKALDGVSVAFRQKEFVAILGPSGSGK
                     TTMLNVIGGLDNYDSGDMVINGKSTKDFKDSDWDAYRNNSIGFIFQSYNLIGHLGIIE
                     NVELGMTLSGVSKDEKRKRAEDALHRVGLTNHMHKKPNQLSGGQMQRVAIARALANDP
                     DILLCDEPTGALDTETSIQIMELIQELSKEKLVIMVTHNPELAHQYADRIIEFSDGKI
                     LTDSHPHIERPKDDQFNLRRTKMSFWTALKLSFNNIRTKKGRTFLTSFASSIGIIGIA
                     IVLSLSSGFQKQIDNTQAETMAKFPITISKVTTNQTRDDAGLGASKADYPDSKTITAK
                     VSEEDKAQHTNKIDQTYVDYVTDIDPNLSNNIGFTRTTGINLLRDVNGKVQPVSFSNQ
                     NPDTESLSLSSTMSAMTGVGVSSFPTQLDTSKENFLKDNYSLLAGSYPASATDVVLIV
                     DGNNNTNINALKNLGFDVKEDEKLDFDDIVGTTFKLVNNNTYYTKLPTGNFIPNTDYD
                     AMYQNASDELKISGILRVKSSSTMNLLSPGIAYSDQLTTQIVNENKESEIVKAQRDSG
                     VNVLTTEKVDENAKQTLLSYLGGDSLPSSIMIYPNNFEDKEKILDYLDDYNKGKSDED
                     KIIYTDLAGTMTELTGGLMDAITYVLIAFAGISLVTSMIMISIITYTSVIERTKEIGV
                     LKALGARKKDITRVFDAETCILGISSGILGVFIAWLATFPINSILYNMTDLKNVAQLN
                     PVHAIILVIVSTILTMLGGHLPARMAAKKDAAIALRAE"
     gene            complement(16627..16938)
                     /locus_tag="ACG8KR_000258"
     CDS             complement(16627..16938)
                     /locus_tag="ACG8KR_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0199666.1"
                     /translation="MKKMEESFPKAVKVENIANILKVTFENGEVKYVKSHWTEEITDA
                     LQFGKKGRGKRKNLLALSRNMWIGTEVTIEADGTVFINGKDRYTPEELWYKGKKSIPE
                     L"
     gene            17119..18594
                     /locus_tag="ACG8KR_000259"
     CDS             17119..18594
                     /locus_tag="ACG8KR_000259"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HFQ0199667.1"
                     /translation="MTDQPYDLRKVIEELKTLPGQYHETDIEIDPEAELSGVYRYIGA
                     GGTVKRPTQEGPAMTFNNIKGFPGTRVNIGTMASRKRVGEILHHDYRTLGRFLKDAVE
                     NPIKPVKVDKADAPAQEVVHLATDADFDIRNILPAPTNTPEDAGPYITMGVVLGSDPE
                     KTMSDVTIHRMVLEDKDTIGMYIMPGGRHIGHFQKQFEALNKPMPITINIGLDPAIAI
                     GTTFEPPTTPLGYNELWCAGALRNEPIQIVDGIAVDEVGIARAEFIIEAEIMPNETMQ
                     EDINTNSGKAMPEFPGYTGDANPAVQVVKVKAVTHRKDNPIMQTTIGPSEEHVSMAGI
                     PTEASILALVDKAIPGKVVNVYNPPAGGGKLMTIMQIRKENEADEGIQRQAAILALSA
                     FKELKTVILVDEDVDIFDMNDVVWTFNTRFQGDKDIIVIPGMRNHPLDPSELPEYNPG
                     SIRFRGISAKTIFDGTVPFDMKEHFVRAKFQEVEDWEKYLK"
     gene            18641..19225
                     /locus_tag="ACG8KR_000260"
     CDS             18641..19225
                     /locus_tag="ACG8KR_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HFQ0199668.1"
                     /translation="MEQLAQRKKKIVIGVSGASGTIYAINLVKKLKEYPMIETHGIIS
                     PWAKQNLKLESDLSLTEFESLFDYHYSNKDLGAAVASGSFLTDGMIIVPASMKTVAGI
                     SVGLGDNLIARAADVTMKEQRKLVIVPRETPLNAIHLENLTKLARLGVQIIPPIPAFY
                     NQPQTIQDLVNHHTMKLLDQFEIVNEHSKRWQGD"
     gene            19227..19610
                     /locus_tag="ACG8KR_000261"
     CDS             19227..19610
                     /locus_tag="ACG8KR_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid decarboxylase"
                     /protein_id="HFQ0199669.1"
                     /translation="MEKETIVEVTKLGFTIQAAVKVIGKDLLITLTGGDTPHIGTVTT
                     FSRDTEMQTVRFPSHDGRFHKDDVLAEKIAVIIKTAVPGSCTITSGIHVDHISNAQIN
                     ASFPMSEELGYKILDWLEKIDFHIK"
     gene            complement(19927..20211)
                     /locus_tag="ACG8KR_000262"
     CDS             complement(19927..20211)
                     /locus_tag="ACG8KR_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HFQ0199670.1"
                     /translation="MREIYSFESKIYASEVGKGGAYIIFPYDIRKEFGAGRVKVQATF
                     DNVPYEGSIVNMGVKNPDGSVCYILGLRKDIRKNIGKEIGDIVAVTVKQK"
     gene            complement(20563..21273)
                     /locus_tag="ACG8KR_000263"
     CDS             complement(20563..21273)
                     /locus_tag="ACG8KR_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HFQ0199671.1"
                     /translation="MIIGVLIVSLLAFALTNVFAKKTWQTFLSLIFGLIFVASLSLIV
                     ANLSNHFGMEKVTETKTEKIVSSADSQGADMLLYKALGNGKEKVYLYRTNEKQEKPKA
                     TGTDNETNKVEKTDGDAEKVTKTTYWEYKNDMYKFWFNIADNNHEYDSRVNTFKIPET
                     WVELSTDQAAKLAELVKKQQSTMESEAKAYVQDGMVKAMTENPKMSKAEQEQRTKALA
                     AEFQQQAFAKLVKEAKGE"
     gene            complement(21270..22742)
                     /locus_tag="ACG8KR_000264"
     CDS             complement(21270..22742)
                     /locus_tag="ACG8KR_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008269079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HFQ0199672.1"
                     /translation="MSNNQPVDIYGKPYNRTLLVVVLLIGTFCTVLNQTLLTTAFPTL
                     MKAFDISASDVQWLTTGFLLVNGIMIPITAWLINKFSSRNLYLSAMTIFLIGTITCFT
                     APNFSTLLIGRLIQACGVGVSMPLLQNIMLSIFPPEKRGSAMGMSGIVIGLAPALGPT
                     LSGYIIDNYHWRDLFGMVLPIVVLVLVLAFFLMKSVIPLSNPSIDILSAILSTIGFGS
                     LLYGFSSVGDDGWGSLKVIGFLVVGVIFIALFSWRQLHLEHPFLELRVFKSSTFTIAA
                     VLAGVTNMAMVGAEMVVPLYIQNIRGESAFHSGLMLLPGALIMGAMMPVTGAIFDKYG
                     AKRLAITGMFILTAATAPFAFLTKTTPVLYIVILYAIRMFGISMVMMPVTTSGMNALP
                     NNLISHGTAVNNTFRQIASSIGTAILISVLTNVTKANIPSKSVLDNTPLAYKDQAINA
                     TLSGYHAAFIVAIIFGILGFIIAFLLKKKEPVLMKAGDNA"
     gene            22880..23353
                     /locus_tag="ACG8KR_000265"
     CDS             22880..23353
                     /locus_tag="ACG8KR_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFQ0199673.1"
                     /translation="MVGASLKRLLENDQLVVGISELSDIVGVSPRQLRYWEQKGFIRS
                     IEADANCPRKYRLPTVIKVELIKKYLDDGFTLSKAAEKAEIRQKKMHHVRKVFSKAIK
                     DIELIDERYTILQIGNFKEENAKMYIIHDNETDELTYKLLPVDEKADYEQLLKEL"
     gene            complement(23506..24057)
                     /locus_tag="ACG8KR_000266"
     CDS             complement(23506..24057)
                     /locus_tag="ACG8KR_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFQ0199674.1"
                     /translation="MARKNTLRYILLGLLSKKKMTGYELNQSFKNEIGEFWQAKHSQI
                     YPELAKMEEQGIIQHQVEITGEKLEKKVYELTEEGKEQLAEWIHTPTNELPVNRDEFV
                     LKLYFVKDVNDPALVEIIQQQRDLHEEKRLHLLERKKTIFPTEKEKHENYGHYLILAH
                     AINRETEYANWLTDVLEESNRKK"
     gene            24162..24692
                     /locus_tag="ACG8KR_000267"
     CDS             24162..24692
                     /locus_tag="ACG8KR_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenolic acid decarboxylase"
                     /protein_id="HFQ0199675.1"
                     /translation="MKEFKELEDFIGTHFIYTYDNGWEYEWYAKNDHTVDYRIHGGMV
                     KGRWVKDQEADIVKLTDGVFKITWTEPTGTDVALDFMPNEKKLHGMIFFPKWVEEHPE
                     ITVTFQNEHIAEMEAAREKYETYPKLLVPEFATITYMGDAGIDNEDVISEEPYDGMPD
                     DIRSGKYFDENYKRVK"
     gene            complement(25175..25618)
                     /locus_tag="ACG8KR_000268"
     CDS             complement(25175..25618)
                     /locus_tag="ACG8KR_000268"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0199676.1"
                     /translation="MIRTTTKLSAKELESVLSIWLHANLDSHEFIPSTYWENQVESVR
                     KALPDAVIYLAEIDQEIIGFAGVIDQFIAGIFVKREYRQQRIGTQLMKKIKETNKTLT
                     LTVYQKNQSAIHFYLSQGFTIQTQQKDEAVNEEELVMKWNQNADF"
     gene            complement(25634..26794)
                     /locus_tag="ACG8KR_000269"
     CDS             complement(25634..26794)
                     /locus_tag="ACG8KR_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFQ0199677.1"
                     /translation="MKQSKQKQSRRLLFRLFLLICIVVGLLIALYPFYVDSLNSFIDQ
                     KRIEQVQKRTAAEIEVQRKKMEEKNQRLTDQGFNPGTDPFNEKNRNESTTSSQLEEWL
                     IGSINIPKIQLNISLYDRLNGRILENGAGVLQGTSFPLGGNSTHSVISAHSGLPNRRL
                     FTELDRLENGDTFILTVLGEKLAYQVKNIQVVLPDDTSVLTISEGEDLVTLLTCTPYM
                     INTHRLLVTGHRIPYNESVKKEEGKGNQERTMRQWFILAGTIIAVVILLVFIGRLIYQ
                     YRLSKKVLDFSFIISDSSGKPVNGSSFILKHKKKTLTRNGVPFSVQSDHHGKVKLDQL
                     PGGTYRIVSADDPKVAVSFGIRKVKQEKIYFFEGRKLVKELQKNGFYFKINE"
     gene            27301..29001
                     /locus_tag="ACG8KR_000270"
     CDS             27301..29001
                     /locus_tag="ACG8KR_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0199678.1"
                     /translation="MMNLSKVCKGVRQLKKYGPFLAIFFIALAFLMPHLFTHKLILGA
                     DAPFHYNRFYDAAQQIKYGDFHYFPSLYGFQQSGRIVNAVYGPFFAYLQGLIVLLAGS
                     WFHYQILSNFILYLISGFSMLILLRYVNVRNWVSVPVAVLFMSTYSIQYWSINQGFTS
                     WGTCFYPLCMIPIADFVLHKKFPVIKVAVSVGLMTQIHLLSSIMLALMYIPFYLYYFF
                     NQTEKGKALLQLIKSVLLYFVLTANIWAGLAIVYSGNKILPPFVNREMSQKAIDLDGS
                     YWLHYPKAFPILILSGFLLFIFFQQKNTLFQKIVLYTSLVFFFLSTSLIPWTTLIEKN
                     VPFISLIQFPFRFFVPFTLLLLLYLALSLNQWSEQKWFRLLSIFGILIFSAQTIQNLY
                     QHLEKWENETFVSRHTYLFDTPEEARKTFFSKDMSTSLFVFQKTTPDYLPIYKETKDN
                     KYDRYNEEILENETTFIKTHPGGLLTIKWKNTGDKTVHIPVIVYDRTVLQQNGKTLTD
                     YEVTDIGTPIVKQQKGINELTLHYQTPIYFYFILSLTLIGWFTLLCLFIYHRYKLLRA
                     "
     gene            complement(29103..29936)
                     /locus_tag="ACG8KR_000271"
     CDS             complement(29103..29936)
                     /locus_tag="ACG8KR_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HFQ0199679.1"
                     /translation="MKFMTLNTHSWLEPEPEKKLQELADKILLEDYEVIALQEINQLL
                     ESEEVEPAKLMKFCPVKNQVPIRKDNFAYRLVQLLKEHGKEYFWSWEMSHIGYDKYEE
                     GNALLTKKTLESQVLTVSQFQKKEDYQTRKILIGKTKIDDQDIFVSSCHFSWWTDKKS
                     GFYFEWKNLENYFLETRVPLFFLGDFNNPVDSQGYYTVRESCLLLQDSYVVANEKGKA
                     ATVEKKIDGWEQNTEKLRIDFIFVPEGMQVKKYQRIFDGIDSPIISDHYGVEIELDVN
                     E"
     gene            complement(30240..32405)
                     /locus_tag="ACG8KR_000272"
     CDS             complement(30240..32405)
                     /locus_tag="ACG8KR_000272"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIBC"
                     /protein_id="HFQ0199680.1"
                     /translation="MKKIFSFEFWQKFGKALMVVVAVMPAAGLMISIGKSIPLINPDW
                     TPLVTTGGVIENIGWAIIGNLHILFALAIGGSWAKERAGGAFAAGISFILINRITGAI
                     FGVTSDMLANEDAFTHTLFGTKIMVKGFFTSVLEAPALNMGVFVGIIAGFVGAMAYNK
                     YYNYRKLPDALSFFNGKRFVPFVVILWSTIVALILAVVWPNVQAGINNFGLWIAESQE
                     SAPILAPFLFGTLERLLLPFGLHHMLTIPINYTQLGGSYEILSGAQAGTQVFGQDPLW
                     LAWATDLVNLKGAGDMSQYEFVLHNWTPARFKVGQMIGSSGILMGMAFAMYRNVDPDK
                     KARYKSMYFSAALAVFLTGVTEPLEFMFMFAAVPLYVIYSVIQGAAFAMADILPLRVH
                     SFGNIELLTRTPLAIKAGLGGDLINFVLCVIAFGVVTYFLANFLIKKFNFATPGRNGN
                     YDNDSEETVVSNSGTGTADQQVVQIIHLLGGKENIKDVDACMTRLRVSVNDREKVGTE
                     EAWKRAGAMGLIVKDNGVQAVYGPKADVLKSDIEDLLQSGAEIPEPEMVSTGNGQAGS
                     KQYLGIEQELVSAASGEVIPLSEVKDPVFSQKMMGDGFAVIPTSREVVAPIAGKVTSI
                     FPSKHAIGLETKDGIEVLIHMGIDTVQMNQPAFEIAVKEGQEVGTGTKLAEMDLDVIQ
                     NEEKDPTIMIVFTNDKVEEVVIKQLGTVTAGKVIGSIKM"
     gene            32665..34959
                     /locus_tag="ACG8KR_000273"
     CDS             32665..34959
                     /locus_tag="ACG8KR_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HFQ0199681.1"
                     /translation="MKQIKRLFAIDPWKISTTQLDKKNLRLQESLTSIGNGYMGMRGN
                     FEETYSGDHHQGTYLAGVWYPDKTRVGWWKNGYPEYFGKVINALNFIAIDISINDQLI
                     DLASLEPEDFYWELDMKNGVLSRTFTITTSTNKVRISFERFLSIVKKESAYIYLTIEM
                     LEGSGTVKLVSKLDGNVQNEDSNYDEHFWNEIDRGTIEEIGYLTTKTIPNNFGIEQFT
                     VTAAMRHFVNGKSVIPDYHEDTLALHGCLSAELSQGETWSVEKEIIVLTSRDIPEAQQ
                     AEKAVSELTNQTNNYIQAKEEQTAAWAERWKLADVVIEGDEEAQQGIRFNLFQLFSTY
                     YGEDERLNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAKPEVTKNLLKYRHNQLPQA
                     IHNAQQQGLKGALYPMVTFTGVECHNEWEITFEEIHRNGAIAYAIYNYTNYTGDTEYL
                     KNEGLEVLAEIARFWADRVHFSKRANKYMIHGVTGPNEYENNINNNWYTNTIAAWVLR
                     YTRENYLTYQKEAAITISEEELAKWKDIAENMYFPVDEELGIFVQHDTFLDKDLMPVS
                     DIPTSDLPLNQHWSWDKILRSCFIKQADVLQGIYFFNDTFTKEEKQKNFDFYEPMTVH
                     ESSLSPSIHSILAAELGMESKAVEMYQRTARLDLDNYNNDTEDGLHITSMTGSWLSIV
                     QGFAQMKTAHDQLRFAPFLPKTWKAYSFHINYRGRLLFISVTDEQVTFELLEGSPLEI
                     YVYEQSYSLADRLEITLRKEEANV"
     gene            34952..35617
                     /gene="pgmB"
                     /locus_tag="ACG8KR_000274"
     CDS             34952..35617
                     /gene="pgmB"
                     /locus_tag="ACG8KR_000274"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HFQ0199682.1"
                     /translation="MFKGVLFDLDGVITDTAEFHYRAWRKLGQEIGITIDHTFNEQLK
                     GVSREDSLSLLLAYGGKEHSFSKEEFAELAKRKNDYYLEMIQTIEPKDVFPGVVPLLD
                     SLKEEKIKIALASASKNGPFLLEKMGLTPYFDAIADPAEATNGKPAPDIFLLAAKAVG
                     LKAEECIGIEDAQAGIQAILSSGAQPIGVGRAEDLGEEIPLVPDTTFLTIDYLKEKWH
                     DHG"
     gene            35610..36617
                     /locus_tag="ACG8KR_000275"
     CDS             35610..36617
                     /locus_tag="ACG8KR_000275"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HFQ0199683.1"
                     /translation="MDNPEISVVPVPETEIKKITLKNDYLEVVLLNYGARLHQIFAPD
                     KEGKSENVLLSYDRFEDVLTDKSFFGATVGPVAGRIRDASWGEHRLEKNCGSHHIHGG
                     TKGWSFQFWEVEVFKTPSSIGVVFYLKDEFSTYPGPITATITYRLTKNELEMTTSASS
                     LVETICNPTNHAYFNLSGNGTRDIYEHQLTVFLDGVLELDQEKLPTGNWTKKEKLPID
                     FRKSPTLQEILACYPDGLDDVFLLHHPRLSKTSLQLFEKHSGRQMTIATSNKSMVLFS
                     TTGFEADFSVNGKQMHSNYGLAIEPQEIPDIVHFPKFGSINLHPGQERISQTIYRFSA
                     Q"
     gene            36639..37658
                     /locus_tag="ACG8KR_000276"
     CDS             36639..37658
                     /locus_tag="ACG8KR_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0199684.1"
                     /translation="MAITVKDVAKKAGVATSTVSRVINDHPSISETTKKKVRKIMDEL
                     GYVPNMTARNLGKRISSAIGVILPPLDSKERIGNPFYLEIMEAINEEARNYDMTTAIA
                     TAKNFELLLENVQRMHLQKQVDGFILVYSDSQDTVIDYLYQNHIPFTLIGQPYHHENE
                     IVYVDNDNQLLGKQATEFLIQNGHKNILFVTNTTHESLYFERYFGYQKAMMMAGLPAL
                     SSVVLEHPEDYLSFEKTLKENEATALVVIDDIFALRTMQLAQMYGYVIPETLSVISFN
                     NSIFSTLTHPYLTSIDIDISELGRVAMQKLRELIHEELSSGVRLVIPHRLIKRETVVP
                     LENPY"
     gene            complement(37939..39306)
                     /gene="nhaC"
                     /locus_tag="ACG8KR_000277"
     CDS             complement(37939..39306)
                     /gene="nhaC"
                     /locus_tag="ACG8KR_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HFQ0199685.1"
                     /translation="MNKNISLKESIAVLIVLLALLGVLIIGFQLSPHIPILAAFMCLL
                     FYGRFKKFTWDEIHDGIIKGITPGIIPILIFLLIGVLVASWILSGTIPTIMVYGFKLV
                     SVRFFLPTAFLVCAIVGVTVGSSFTTISTIGIAFLGIGHLLGFNDAMTTGAVVSGAFL
                     GNNCSPLSDTTNLAAGIGGVNLFEHIAGMRYTDFPAFVLSLIFYTILGHNSHSADLAS
                     INEMIQNMKASFWISPWTLIPLVILFGGAIKKIPAIPTLLAGSTAALVLSFIHEKSLT
                     VGKAADILMNGYVANTPDPKLDELLSRGGIMSMLGSASLILLALALGGLLIKYLIVDT
                     IVQELKEKMDRPSRLVGFTALSCIGINLIVGEQYLSIILPGETFKRSFDDVGLSKTYL
                     TRTLADSGATVNSLVPWGVSGTFIMGTMKVSALHYLPFAFFSILAPVFTILGGFLLNR
                     RRVKN"
     gene            complement(39415..40836)
                     /gene="tyrP"
                     /locus_tag="ACG8KR_000278"
     CDS             complement(39415..40836)
                     /gene="tyrP"
                     /locus_tag="ACG8KR_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070908 - tyrosine:tyramine antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-tyramine antiporter"
                     /protein_id="HFQ0199686.1"
                     /translation="MSKSEVSKMGLGTFIGLTMALCATVRSIPTLAAVGWTLIFYSIF
                     AVLFFAGPISMVSGELSTMLPQEGGPQLWVKTALGSKWGFVVAWLLWVQMFPGMVMVA
                     STLGPLLGNTFGNVELGNNHWFVLGCILVIYWIITILNLKFDMAKVGGNIGVWLGVYI
                     PVVIMFVLGVLAAFKVGLVSNGYLGDFSWSKAFPDLEHIDSLKYLAGITFIFVGIEMS
                     SVYMPRLKDATKNYTKGVFIALIGLVLLNVINAMLVANVVPDGKMELANITQPILIDC
                     QILGLPEVIGNIFSFMVFIGVLLQLSAWVTGPSKTIIQVAREGFLPPKFGFHKENKYG
                     VSRNVVLTQSIVISLFALLYGVMDDVSAVFLTLTNATTVIYCIVYILIAVSLLKMRKK
                     HPEFERPYRIGKNGNGLAWVVSCMLIFSIIVVVFATLGTATLSDALLVAAITVVMFVI
                     PLIINHFKKDSWGIEVEKSLEEK"
     gene            complement(41079..42956)
                     /gene="tdc"
                     /locus_tag="ACG8KR_000279"
     CDS             complement(41079..42956)
                     /gene="tdc"
                     /locus_tag="ACG8KR_000279"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004837 - tyrosine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HFQ0199687.1"
                     /translation="MSESLSKDLNLNALFIGDKAENGQIYKALLNELVDEHLGWRQNY
                     MPQDMPIITPEEKSSASFEHTVNKTKDVLSEISARMRTHSVPWHNAGRYWGHMNSETL
                     MPSLLAYNFAMLWNGNNVAYESSPATSQMEEEVGMEFAKLMSYKDGWGHIVADGSLAN
                     LEGLWYARNIKSLPLAMKEVTPELVAGKSDWELMNLSTEEIMNLLDSVPEKIDEIKAH
                     SARSGKHLEKLGKWLVPQTKHYSWLKAADIIGIGLDQVIPVPVDHNYRMDINELEKIV
                     RGLAAEKTPILGVVGVVGSTEEGAIDGIDKIVALRRVLEKDGIYFYLHVDAAYGGYGR
                     AIFLDEDNNFIPFEDLKDVHYKYNVFTENKDYILEEVHSAYKAIEEAESVTIDPHKMG
                     YVPYSAGGIVIKDIRMRDVISYFATYVFEKGADIPALLGAYILEGSKAGATAASVWAA
                     HHVLPLNVTGYGKLMGASIEGAHRFYNFLNDLSFKVGDKEIEVHPLTYPDFNMVDYVF
                     KEKGNDDLVAMNKLNHDVYDYSSYVKGSIYGNEFLTSHTDFAIPDYGNSPLQFVNQLG
                     FSDEEWNRAGKVTVLRASVMTPYMNKEEHFEEYAEKIKAALQEKLEKIYADQLLASEA
                     K"
     gene            complement(43247..44503)
                     /gene="tyrS"
                     /locus_tag="ACG8KR_000280"
     CDS             complement(43247..44503)
                     /gene="tyrS"
                     /locus_tag="ACG8KR_000280"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HFQ0199688.1"
                     /translation="MNIIDELTWRDAINQQTNEERLRELVEEKSISLYCGVDPTGDSM
                     HIGHLIPFMMMKRFQLAGHHPYILIGGGTGTIGDPSGRKTERVLQTMEQVQHNVDALS
                     NQMRKLFGKDANITFVNNYDWLSKISLLEFLRDYGKNFNINTMLAKDIVASRLEVGIS
                     FTEFTYQILQSIDFLHLHKTYDVQLQIGGADQWGNITAGLDLIRKLEGPEAEAFGLTI
                     PLMLKADGTKFGKTAGGAVWLDPKKTSPFEFYQFWLNQDDRDVVKYLKFFTFLSQEEI
                     EDLAKKVETEPEKREAQRRLAEEVTRFVHSEEDLKEAQKITQALFSGNIKELNAEEIA
                     QGFGKMPNVEISSTPENIVELLVSTKIEPSKRQAREDVSNGAISINGDRVTDLNFVIN
                     PSDEFDGKFVVIRKGKKNYFLAKVID"
     misc_binding    complement(44615..44820)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            45083..46384
                     /locus_tag="ACG8KR_000281"
     CDS             45083..46384
                     /locus_tag="ACG8KR_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFQ0199689.1"
                     /translation="MEKKQKIILGATSALTIVILILSLFFVIRSSTKKEPIIPEKKIT
                     EKAKEPTDDKQQLVTQAKNLAQSYDYDQAIALLEKDSKNGSTETQQLLQTLKKEKEQL
                     IDWSDPTQISHVFFHSLIVDPQKAFHSQQAQGYKDYMVTVEEFNRSIEQLYQNDFVLV
                     NLNDLIQKDEQGNLSFNGLALPEGKKPLVLSQDDVSYYEYMDNSGFPSRLVVSNNNEI
                     KNVYKEKDKEEIGDFDMVPLIDAFVKKHPDFSYRGAKGTLALTGYNGVLGYRTSKSQY
                     GDNEKTKKEIKEAKKVAEQLKKDGWSFASHTWGHLNMTQASLEDVKKDNELWQQEVAP
                     ILGKTNILIYPFGADISDWQPYSAENQKFAYLKSQGFDIFCNVDASTPAWGQLGTDFY
                     RNARINIDGIRFQSDLDGKNPILDPFINVHDVYDKEARESA"
     gene            complement(46453..47052)
                     /locus_tag="ACG8KR_000282"
     CDS             complement(46453..47052)
                     /locus_tag="ACG8KR_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFQ0199690.1"
                     /translation="MNGFEKRAQEKKKEILQAAFDLMNTDIGTAGVTMDKVAENAQVS
                     KATIFKYFGSKEGLIYEVFFAFMNDLGQEAKALIAENLPFEETLIAMSENKIRYLNRV
                     NKQFYIDLMTYYTKKEKKEFSDAMDAYTQESFSIMLDLFHRGRKEGKVDLKYSDEFLI
                     LYFQAMVEGISKPEIYAKLLPYTAEWTEVLIKGIAPKKK"
BASE COUNT        14506 a         9735 c         8381 g        14611 t
ORIGIN      
        1 cttgtttatc cccaaacaaa ggtggtctat gcaataaaat gaacgtcaaa gaaaaacgaa
       61 tgaatgctcg ttgcttattt acaaattgtt ttatttagaa tacacagaca gatttatgaa
      121 aagaaaaagc ttttcgacac gattatctgt tccaaaaaat gtctaatgaa tgctgaataa
      181 gacagaaaat caatgaaagg aaaaggacta gtttattatg aaaaataaaa aaacgatacg
      241 tttgctagct gctacttggc tgattgctcc gtctgttttg tcgaccaata ccgtatttgc
      301 cgtagataat caaatagctg atcaaacagt cacaacagat acgactacat ccagctcagc
      361 tactagtgaa acaacggatt cttttacaga agacccaact ggaacaacta actcaacaac
      421 aggatcgacc gagccaactg aaattgatcc ggctgatcca tcagaaaatg aaaagccagc
      481 cgagacatat tcccttacaa ttgctccttc tttgattggt aagatccaac tgactgcttc
      541 gacagcggat tcccctgaac agcaaccttt ggatattacg gaaagtggaa cggtagaagg
      601 tttattagcg gaaacgaagg taacttacac tattacgcca gctgaaggtg aacaaattgc
      661 ttcagtcgct gttaatggtg ccaaagaagt aaattatgat gcaaagacct ttacagtagg
      721 tacgaacaat ttatctatta ctgcagaatt taaagctgga ccggatactt caacagagaa
      781 accaactgat cctagtgaag aagtccctag tacaccagat ccggaaacgc caaacaaacc
      841 agatgtgggt aataacaaca ataataatgg ttcaaataat tcaaacaata ataacaataa
      901 ttcgaacagc gggaattctt cgaatcaaac aaacccatct ggatcgaata acaatggaaa
      961 tactcagaga gaaccaaacc atccaaacaa tggttcatcg aatggcggaa cgaacaatgc
     1021 ttcatctcgt ccaaatacag caaatcaaag cggtatagag aatccatctt ctgcttcttc
     1081 tgattttgtt gtaaaatcac caattgatgc tgtattgcct acaaatacaa cgaatgtcca
     1141 acaagcaatc gttcgagaag cattcaagca tcttggaaaa ccatatgtat ggggagcaaa
     1201 aggtccaaat acttttgact gttctggttt gacttattac gtatatatga aagcaaccgg
     1261 tcattatatc ggtggctgga caggcgaaca gcaatatgca ggtacgcaga ttccagtaag
     1321 tcaggcccaa cctggcgacc tcgtcttctg gggaccttct tccggtgtta ctcaccatgt
     1381 aggtatctat attgggaacg gacaatttat ccatgcgcct caaccaggag ataaagttcg
     1441 agtaacttct atttcagatt tcacgcctga ttttgctgtc cgagtaaact tagcaggttt
     1501 acctgctgct tcaggttcgc tgacaggcgg ctcatctatt ttagatggat tagatggctc
     1561 gttccatttc agtcaaaacc aaacaactga tcagttcttg aaaaagattg cagatgatgc
     1621 acaggaaatc gggcaaaaag aaggaatcta cgcatcagtc atgatggctc aagctatcct
     1681 tgaaagtgga tctggaaaca gcttattatc aagcgaacca aatcataacc tttttggtat
     1741 caaaggatca tataaaggaa gcagcgtgac cttcaataca cttgaacaag atagttccgg
     1801 acaaagctac caaatccgcg cacaattccg taaatatcct tcttacaagg aaagtttgga
     1861 agactatgcc gacttgatca aaaatggact gactggcaat cctgatttct acaaaccaac
     1921 ttggaagtcg gaaacaaaag actacaaaga agcaaccaaa tatttggaag gacgttatgc
     1981 tacagatcgc caatattcac aaaaattgaa cgcgatcatc gaagcgtacg acctgacaaa
     2041 atatgatgaa ccgaaaaaag aagcagcaac aactgaagaa gcaaacgctg aaatcctcaa
     2101 tatgtctaag cgattcgatg tgccaatcaa atggacaaca gatggcgtgc caatcacagg
     2161 attagaattt tcaacgataa aacgcgtcaa caacgcttct tccatcttcc gtgtcgcttc
     2221 aatctgggat ttgtggaaca attttacggc aagacagata ccagaaacaa ctactcgtac
     2281 agttaaagct gcaactgttc ctgtactaac cttgttaagt ttagacaaat cattctggct
     2341 tgatctgcct ttaaagaaat agaataaatt aaaaagggga tgtgacactg tcacactccc
     2401 tttttaattt attctatttc tttatattta ttttgtattc cgttacaaac tcgcgtactt
     2461 ggttaggttc cagagtgaca tctccaaaac tttcaagtcg ctccgctcct ggcagttctt
     2521 gagtctcaaa tgtgataccg ccatgattta ctaatttatt cccgcgcatt tccggtccat
     2581 cttctccaaa atttgctgta aaaatcacga ttcctgaagc atctgttttt acttggatcg
     2641 atactttttc atctgggctt gtcaattccg cagcatttag taaaccagat tgttttttca
     2701 acaaaaatgg atgatcgatg ccgcccacta tcattgattg gcgaaaatca ctggcaaaga
     2761 cctcacctaa ttttttagga cgttggaagt caaatgctgt gccacttact gaaactactt
     2821 ctcctgtagg cgttgtatct ttattgagcg tagcaaataa tcggctattc aaccaaagta
     2881 tatgttcgtc gattgactgt gctggatcac cggttaaatt gaaatagaca tgatttgtag
     2941 gattgaatac tgttttgcgg tcactgatcc cttggaccgt cactcgccat gtatcatcgt
     3001 tcgtcagcgt ataccgtact tctacatcta aagttccagg aaatccgttt tccatatctg
     3061 ggctagtctg atagaaaatg accgatgctt cgttttcacc attgatgact gtataatgcc
     3121 actgcttggc ttcaaagcta ggtgcaccac catgtaaaca atgaccagtg tccgggtctg
     3181 tttttacttc gaacgtttca tcatctaatt tgaatgtgcc attcgtaatc cgtccagcca
     3241 tacgtccgat tgaagcaccg atataagggt ctttttctag atattctttt gcttcatcaa
     3301 aacctaagat caactctcgt ttttcattat cgattattgt ttgaaaatga acaacccgtg
     3361 ccccaagatc actgacagac aaagatgtcc cattttgatt cgtgatcgta atcaagtgat
     3421 agttttcgcc aaaattttgt tcattgattt ccatgttcct tgcaccctct tttctttcat
     3481 ttgcactgat tccagtatac atggaaaccg ttttcttaac agcttattcc gtgagtaacg
     3541 agaaaaaatt tactatattc taataaaaga ccgtgaaaaa aaggttgtga caaaaatatt
     3601 gtcacaacct ctttttccga acaaacggtg ttcaaaagct tgcatctgcg gtaataagcc
     3661 caaccaaacg aaaaatatga ggagctattt ttgcttggct gttcttatta cttgatgcag
     3721 gacagctttt tcacaatctc tttttcaata cgattccagt atataaccat gtcgtcagta
     3781 catagtagat gaaatacaat acgaagaata tcgtaaacgg aacccaaata tctttgtatg
     3841 gattttgcat gaatagacca aacatttgta atccgaataa gacatggact acacctaaga
     3901 ttcctggtgc taagaataac acgcctactt ctctacggat cgattgttta agcaacccgc
     3961 gtcgtgttcc tatcttttca agcatttcat aacgagcaat gtcactcttg cttcctgaca
     4021 agatcttgaa catcaaacag ctagcaagca tggtcaagaa agctaaacct aggaagaatc
     4081 ccataaattc aaaaccagag aatagaccat tatacacttc atacatttca tatttctggt
     4141 tgctgtactc tactttttgc aaagatggat tgttcatttt attttgagca acaagtgttt
     4201 cgattggttt caggtttgct tggaagtctt ttacctgaac tagctgtaaa gtggattgtg
     4261 gcaagttcag ttgatcaaat tctgattgac tgactacctt gatttctttt tcttgctgtt
     4321 ccggcaattc atagcttcga agttcctctt gtacagtcag ttcattcatt tttttagcag
     4381 gaactttttc tttcgtgatc ttcggcggtg ttccatcttc atgttttatc atgatcaatg
     4441 gttgagccgc aaattcactt gcattatagt aaatcgttcc cgcatcttct ttttgagtat
     4501 aagaagcaga aagtgttggt gaaagttctt tgattttttg ctgatcttct gcccgaggat
     4561 tattcatcac tagatcatat ccagaattgt tttctgtcaa gtctgcaatt tcatttttaa
     4621 aacctaatcc taccgtcaaa gcgcccagtg ccaaggcaaa cagcatagcg accattgaga
     4681 gcatttgtgt atattcacga atacgaaagt ttaactgtga caatgtgaaa ttgttcaatt
     4741 ttttcaatga aatctgttct gatttcttta gtaatgataa aaagaaaatg atcacagaat
     4801 ggaaaatgaa ataagttcct aatacgatcg taactaaggc tacccctata ccaaagactt
     4861 gataggtccc cacgtgtttc atcatatagt aaccgcagcc tagaaaagca attcccagta
     4921 ctacttcaag gaaaaacaag aatttatgct gcttgattct tgttggtgtc tgatccgcac
     4981 gaatcaatgc taaaatcggt tttttgacaa tcgaaaaagc attgacaaca gcagctaata
     5041 aaaacaaaac agcaaaaaac aaaacagtga tcaacaaccc gtgaatacta aaaggtgtaa
     5101 aatgcgaaat attgagatct aactgatcca ctaataatcg gtggacgata cttgtcaatc
     5161 ctacaccgac agctgaaccc actaaagtag ctgcgatccc tactaagaat gtttcagaaa
     5221 aaatcatctg tgcgattttt ctgccttttg ctcccagcat cataaacatt gcatagtctt
     5281 tttgccgcat cgtcatcaaa aaggaatttg catataaaat ataaacgaat gtgatgatcc
     5341 ctagtaaaac agaaccgaat cggaaaatga taactgtagc cgcaacgatc gtattacttt
     5401 tcaaaaaagc ttcgttgctt gccattgatt caaacatgta aaaaatagca gatgccataa
     5461 ctaatcctga gaataacacc aagtagtctc ttaaacggct cttgatccca gtaagtgata
     5521 atttccaaag catagtcaat ctccccctac tgttccaagt ttccaagaat agatagtatt
     5581 tctcgataga aggaatcacg gctttgatct ccacgtttca cttcttgatg gataccgcca
     5641 tccttgatga ataaaatacg ttggcaataa cttgcagaaa acggatcatg cgtcacaaga
     5701 agaatcgata catgatcttt tgtattcaat tcgtccatcg tatctaaaag atcccgggca
     5761 cttttggaat ccagcgcacc tgtaggctca tcccctaata gaatcgttgg ttgtgtgatc
     5821 aatgcacgtg cggcggcgac ccgttgtttt tgtccaccgg aaatctcaga aggatagctt
     5881 tccaaaatat gagagatgga taaacgttct gcaatttttt ggacacgttg cttgatttct
     5941 tttggtctga cgccttgtaa agaaagagga acagcgatat tttcttgtgc tgtcaagctt
     6001 tctaataaat tgaaatcttg gaaaataaag ccaatctcat tcgcacgaaa atctgctagt
     6061 tgatttcctt ttaatgtggt cacatctttt tgattgattc ggatatgccc atctgttggt
     6121 ttatctaatg tggataaaat attcaaaagt gtcgacttac cagaaccaga agcgcccatg
     6181 atcccaataa attcgccttt ttctacatcg aagctgatgc cgcttaatgc ttgcgtcttt
     6241 ttctcattgt tttttccgta tacttttgtt acattctgta cttcgactat tttgttcatt
     6301 tctttcccta ccctttaatt agtttttcca gtgctgtttt cggcacgtct tttttttcga
     6361 cttcttcata cgaagtgaca cctttatttt tgttccaggt gatttttaaa tatgctcctt
     6421 tacgaagcgg tcgagtcaat tgtcccttga agtccagttc tttttcctta ccctctttcg
     6481 tatagccagg caggatatat ctatagttga ctgtttcttg tccagagtct gattttgaaa
     6541 ctgtttttgt accatctgtc gtaatttgta catagtattc ttctccaccc ataactacgt
     6601 aatctgcgat tttcacccct gcaagtacca caacgatgaa aacaagtaga ccgatcagaa
     6661 gttttttcac tttttcccct tctctcttac tttatatact gattatagaa aaaagcgaac
     6721 aattctgcct tcgaatcaac tatcattctt ctttcaattt tgtcattttc tttaaaacaa
     6781 ctaaaaaaag agttcaagac aataatccgt cctaaactcc ttcttcctat cttacaaaaa
     6841 gtatgtcact tacggtcttt catgtcgcct gtattgataa caggttgcgt tcctttgtct
     6901 gtaatgattg agacaagtaa attattgatc aattggactt tccgatcatc ggtgaaattg
     6961 atttcttgtc catcttcgat ttgctctaag gccatttgag tgattgtgac agctccttca
     7021 acaatggttt gtcgagcaga caaaatggct tttgcttgtt gacgttggag catggcacta
     7081 gcgatttctg ttgcataggc taaatggttc aagcgtgttt cgattacttc gacacctgct
     7141 actgctaaac gctcttgcag ctctttttgc aactcgtcag acactgccgt cgtgtttcct
     7201 cgaagcgtca aatcattctc attgaatgta tcatacgggt attggctagc gatatgccga
     7261 atggctgtct cactttggat ttctacaaaa tcttgataat acgctacatc gaataatgcc
     7321 tttgctgtat caacgacttt aaagactact actgctgaga tttcgatggg gtttccatcc
     7381 aaatcattga cttttaaaac agaactattg aaattccgaa ctttcaacga aacagtcatt
     7441 ttttgtgcca aaggtatcgt aaggaaaaag ccattttctc gaatcgtacc taaatattgt
     7501 ccaaaaaaca aaattacttt tgcttgatta ggactgacta cagttgccga acttaacaac
     7561 aataacgtga tcaaccaaag aaaaatactt aaaaaaagtg cccaaacttt cgcttgccac
     7621 atacctaaat agaagaaaaa caatccaact aaagctaata cgaccaatcc gacaatccct
     7681 gcataaccat tgatataaaa tgctcttttt tctctcatgc tacttcctgc gctcctttat
     7741 ccttcaataa atatctaatg ttactttttt aatttttttt tcgaatacgc gccattacac
     7801 cttctagttc taactctttg tcttcagtga tgtaccaacc gcttgcctgt gtccctccgg
     7861 ctgcctgcgg taaataatcc aattttgttc gaacccagtg cttttcttct gtaaaatagt
     7921 cttctaccaa gacctctaga tccatccctg ctgaaagagg caaatcttcc cgaacagatt
     7981 taaagataaa tcgttccttc ttcccatcat acttcaatat tcctgtatcg ataatcggta
     8041 aagaataata atccagctct ttcttcaccg tcaataaatg tttgaaaact tcatggtaca
     8101 cttgctctaa ttggcgacct tctgaattgt tcgggtaatt aaccatcact ttttctttgt
     8161 ctggatcata gaaggtttgg cttagttgag tgatataagt atgtaatctt tccatttctg
     8221 tttttaactt ttctctttga ccttcttcta ccttcatata ctctttcacc ctgttttctt
     8281 tttagatatt ttctttctta ttttactata gttatgtgcg ttaagcgatg aaaaacagca
     8341 aagattggaa ggatctttgc tgttttttct taaaatatcc agttgagtac aggatcaatc
     8401 tgtttcattg tcctcttgat ccgtcgtaat attgatcgat gtgtctttag cagtgatctt
     8461 tagctttttc tgtccttgtt ctaacaaagt atttgttatt ggctggtagt ctttttcaat
     8521 ataagattca ccagacgttt ggatagtata gccactgttt tccatctcat gcaaagataa
     8581 attgccaccg tttaggatga cttttccttc tttgctgatc aaactgtctt ctagatggaa
     8641 gttgctgttt tcacctgtga cgatcaagtt atccacattg gcctcatcta atcgaagtga
     8701 gcctaattca tctttcgcag ttattgttcc tcctgtaaat tcattgaacg atacccagcc
     8761 atcatgtgtc ttagtattca cttttttgat tgatacttcg ttgatatcaa ttgcactatt
     8821 attcccttcg attgtcaatt cagataattc atgatctgca gggacaacaa tagtgatttg
     8881 tgcacttgtg tcttctaaac taatgattgc acctgttgat tttttgactg cattcacagt
     8941 caacacgcca tttttctcct ttatttctgg cttttctgtc ttatcataag tcgtttttat
     9001 ctcaaaatcc aatccgcgta tgaccgaaac tgtttgattt tctgcatgga tgacgatttt
     9061 gtttgcatcc ttgatttttt ttgtctcagt aaccatatcg atcaaccgag gaccattatc
     9121 ccaagtcaga ctcttttgag cgcctaatgt ataagttgca cctgctagga ggccaccagt
     9181 aagcatcaga ccaataccgg taatcaaaaa atgtttcaat ctcaatttat gtgtcctcct
     9241 tcaaacgccc gctgaatgca gatctctttt tgtaataaaa cgtcgtccca tccatttaac
     9301 aaagttcatc atgacataaa aaagcccttt tgttcctttc agcaacagtg gcgaaataaa
     9361 tactgcgctt cctgtggcga acagaccgac accgataaag aataatccac tcagaatcga
     9421 ttgtccaaac acactgattc ctgagatgat catgaatagg ccaacaccta agatactgat
     9481 acataaaaca tagatactaa aaatgagcgc tagtaaagca accaatagtc ccccaataac
     9541 caatataaac cccagtgcta gtggaatgag gacaggagaa gcaaataaac cgataatgat
     9601 taaccaaatc aggcgaattt gtctttttgg atttttcact aactcgtttt ctgcttgttc
     9661 ttcttctgaa aagtaactga tttttaattt taaggcaaac tgctttggtg taccatattc
     9721 atcaatgatc tgatctaatg tttttccaga atctaaaaaa tattcctcat aatactggat
     9781 caaatcaaaa cgatcgtctt caggaatttc ttgaagatga tccacaactt cattcaaata
     9841 ttcattcatt tgtgctctcc tccaataacc ggtctaaaga atctcgaaaa acaatccatt
     9901 catcaataat ttcttctaaa tgacgacttc ccattggtgt caacgagtaa tatcgacggt
     9961 ttctgccttc aaaaggttca tcataggtct caagatatcc ttcttttttc aatcttctca
    10021 aaaccggata tagtgtagat tcactgacag agagactttc ttgtacgatt tttgttaagg
    10081 catacccata catatcttcc ttgcttaatg ctgctagcac agtaccatca agaagaacag
    10141 ttggaatctg gatgtgcatt acgtcacctt ctattctcaa gtgtaatatt ttatttcaat
    10201 ctatattata caacatataa tatagtttca aaatcagttt gtcaaataca aaaaaactga
    10261 ctaaaagatt tgatcctttt tagtcagcaa aaaatggctt ctctttgatt tttattataa
    10321 cagtggcata atccaatttg tccacatcca gctcagtggc atgataacga tcattgcagg
    10381 tatcatgtca gctactggga acattttcac gttgatcatt ctaaaaccgg tagcgaccat
    10441 caagaatccg ccgcaagctt taaaatctaa gatcatgtct ggtgtagtca atggataaat
    10501 aaattttgcc aataagaaca aaatataaaa aataatgaat tggggaattg ctatcacaga
    10561 aacgacatac cctaaattac acgcaaaaat agctgctgtg aaaaaatcaa ggacagattt
    10621 agaaatcaaa atcgtactgt ctcctgtcat cccagaatct aaacttccat agatccccgt
    10681 accacttgca caaaatagaa caatgaccgt cactaatgtg ctgataaatt catcgtgggt
    10741 cataccaagc ttttcactag ggaagatttt agaaataggt acttgcatca gcgcagcccc
    10801 cttatttatc ccattaccta aatgaacaat caaaccaatc gctgttccta tcactaaagc
    10861 aaaaataaca gccggcatat ttttcatcgg cgcaatggaa tagattccca tccccatcga
    10921 acacacacca aaaaccatcg tcagctgtgc tttaaaatgt tcacttaatt tatttcctac
    10981 taatccgcca attagtccgc caaacaaaac ggaacctaca ttgatcaaaa ctccagttgg
    11041 catcaaaaaa ctcctctctt tttctacgaa gcgaaaaaaa tcagaaaaat ttctgtaatt
    11101 attgctttcg ctgcaagaaa gattgtaaca gacagagaag attacaccaa ttcatttttt
    11161 gtgttaaagt attctttatt ggaataagta ggaggaatat gatggatatc cataaattcg
    11221 aagtttttct tgatttagcc gaaacaatga atttcaccaa aactgctgat cgacaattca
    11281 ccacccaagg aaatatttct aaacagattc tttcgttaga aacagaatta gacgtcaaat
    11341 tattcgaacg cgcccataga aaaatcgaat tgaccgaagc tggaagctta ttgctcccat
    11401 atgtaaaaaa tgtcgtagaa caataccatg ctatgcaaga agtgcttgaa acctatacga
    11461 aagacaacaa tctttcatta aatatcttaa cgataccgac aatgatcaat tataaaggtt
    11521 tttccaaaat caccgagttt ttgaaacgcc atcctgaatt tacagtccag ttgaaagaag
    11581 tggaaagtgt cgaattaact tacaatgacg ccaatgaaca tagtgacacg atccactttg
    11641 ctagaagttt ccaggcccca ccaagttcga ttgagtggtt gcctactgaa acagacgatt
    11701 ttgtagcagt tctacctaaa aatcatcctt tagctagtaa aaagaaattg gatctaagcg
    11761 aactgaaaaa ggagaacttt ttgctccttg gccccactac gaacttgtat caacctgtac
    11821 tagatctgtg tcgcgaagct ggatttgaac caaaaatctc ttataaagga gcaagaatcg
    11881 acttgatcat caatatgatt gcaaatgaca tggggattgc tattgttatg gaaaaaacag
    11941 tcaagaatct attaaaagaa aatactgtga ttctccctat ttctccaaca acagagagtt
    12001 atcttgcatt tactcgtaaa gtcggtgagc attctttggc ttcagataca ttttgggagt
    12061 atttaaatca agaagaaaac taagccctac attttctgtc catctatcca cccaaaaaaa
    12121 gagagcgacg catgactttc gctctctttc ttgcatcatg tccttgtttc aatgacacct
    12181 ctaacatttc tcgtagttca ataagaaagc agcgattttt atgccaaaag aaccgtaaga
    12241 acatttccct aatcacaaaa aaacgactaa aaacccctaa aaaccttcaa tagaattttt
    12301 aggggtttta ttctttttcc tattgtttac ttaaccgtat cattgctgtc gttcctactc
    12361 cttcgatact gctataatca atacgtccat catgcagttc tagaattttt ttaaccagtg
    12421 aaagtcctag cccattccca gaaatttgac gacttgtatc tccttgataa aatttttcaa
    12481 acattcgatc tctggtctct tcattcattc ctacacctga atcggtcact tccagtacaa
    12541 tctctgtggc tgtctcaaat agtttgatat gaatctgtcc attgatttga ttgtacttta
    12601 tggcgttatc catgatattc agccacactt ggtacaaaaa ctcttcattg cctgtgtaat
    12661 ttactgcagc aagttcgata tccaattcta gctgttcttt ctcccattta ggctgaaaaa
    12721 aaacgattac ttctctgatt tgttcatcta atcgatactc tttcttttcc agttggatac
    12781 gctggttttc cagtttgttc aattttaaga tgttctctgt taattgtgaa agctgcgtga
    12841 tactttcgat aatccgatat aaaaatattt ggcgttcttc gtcagagaga tttggggctt
    12901 gtaacaattg aacatatcct tggattgttg ctagtggtgt tttgaattca tgggatacac
    12961 tggacacaaa gtcattcctc agtgtctcga tactattgag ttcttggacc ataacgttga
    13021 aatctttata cagttgctgt acttctgcta ctttctgctg ttcatccatt cgaatcgaaa
    13081 aatctcccgt tgctaccaga ctcatatttg tccctagcgc actgattgga tgcaggatac
    13141 gccgcccaac aaaaatcgat attcctgtac ccacgaccat actgaacccc gcaaagacga
    13201 ataacgggta taacgttggt tttccagccg attcagcagg cgagatagaa tgtcgagaca
    13261 ataagaaaat aaaaccgaga aaacacagca gtgtcactaa gatcgaaatg aagacaatac
    13321 tagcaaaata gatccataat tgtgaaaata gctttttttt catctttaaa taaccgcctt
    13381 atagcctagt ccccgtactg tgatgatttg aaaatcttcc gtcttttcaa aacgggtcct
    13441 taaacgtttg atatgtacat cgatcgttcg ttcttctgta ttcgtatcta atccccaaat
    13501 atcatccatc aactgttggc gagtgaagat cttgttggga taagacagta atttatacag
    13561 aagcagaaat tctttttgag gcagaatcaa cgaatgctga tcttgtaaca ctgtataagc
    13621 atctagctct aattttgttt gtccgattat caagctactg ctgtaattga tctttgaacg
    13681 tcgaagcaat gcttccacac gtaggatcat ctcattgaca tcgatgggtt ttgtcatata
    13741 atcatctgct cctaatttga acccgcgttt cttatcctca aaatcggcct ttgccgtaat
    13801 cattagaaca ggtgtgtcaa atctggtgct acgtaatgag tctagcaagt catagccatc
    13861 cattttcggc atcatgatat ctgtaatgat taattctata cgtgtcgctt ctagtttttt
    13921 cagcgcatcc tctccattca atgcggtgat gactgaaaag cccgctcgtt ccaacacaga
    13981 acggtaaagc aactgtaatt gcggatcatc ttctactact aaaatacgat tcattgatct
    14041 ccctctgttt tttgaaaaat ttctagattt tttcttgtag ttcatcttca gttaacattt
    14101 atttcgtata ctacgatcat tgttctgata tacttattag tataccgaat atttcaatac
    14161 gggagaaggg tgattatgct tcaattaaaa gatataaaaa aatattataa agtcggcgaa
    14221 accaccacca aagcattgga tggtgtgtct gtcgctttca gacaaaaaga attcgtagcg
    14281 attttaggtc ctagtggttc tggtaaaacg actatgctga atgtgattgg tggactagat
    14341 aattatgatt ccggtgatat ggtaatcaac ggaaagtcta ctaaagactt taaggacagt
    14401 gactgggatg cctaccgaaa caactctatc ggtttcattt ttcaaagtta caacttgatt
    14461 ggtcatctag gcattattga aaacgttgaa cttgggatga cattaagtgg tgtttctaaa
    14521 gacgaaaaac gaaaacgggc agaagatgca ttgcaccgag tcggcttgac aaatcatatg
    14581 cataaaaagc caaatcagtt atctggtgga caaatgcagc gagtggccat cgctcgagca
    14641 ctagccaatg acccagatat ccttttgtgc gatgaaccaa ccggagcgtt agatacagaa
    14701 acaagtatcc agatcatgga attgatccaa gagttatcga aagaaaaatt agtgatcatg
    14761 gtcacacata atcctgaact ggcccatcaa tacgcagatc gaatcattga attttctgat
    14821 ggtaagatct taacagattc ccatcctcac atcgaacgac caaaagatga tcaatttaac
    14881 ttacgcagaa caaagatgag tttttggaca gcgttaaagc tttccttcaa caatatccga
    14941 acaaaaaaag gacgtacttt cttgacctcc tttgcttcca gtattgggat catcggaatc
    15001 gcgatcgttc tgtccctttc ttctggattc caaaaacaaa tcgataacac gcaagctgaa
    15061 acgatggcga aatttccgat tactatttct aaggtaacga ctaaccagac tcgtgacgat
    15121 gccggattgg gtgcaagcaa agcggattat ccagacagca aaacgattac tgcaaaagtc
    15181 agcgaagaag ataaagcaca gcacacgaat aagattgatc aaacatacgt cgattacgta
    15241 acagacatcg atcctaactt aagtaataat atcggattta cccgtactac aggaatcaat
    15301 cttttacgtg atgtaaatgg caaagtacag cctgtcagct tctcgaatca aaatcctgat
    15361 acggaatctc tttctttatc tagtacgatg tcagcaatga caggtgtggg agtgtcaagt
    15421 ttccctacac agcttgacac tagcaaagaa aactttttaa aagacaatta ttctttactt
    15481 gccggaagtt atccagcctc agcaacagat gtcgtgctga tcgtagacgg caataataat
    15541 actaatatca acgcattgaa gaatcttgga tttgatgtga aagaagacga aaaacttgat
    15601 tttgatgata tcgttggtac aacattcaaa ttagtaaaca acaatactta ctatacaaaa
    15661 cttccaactg ggaattttat ccctaataca gattatgatg cgatgtatca gaatgcttct
    15721 gatgaattga agatctcggg gatcctacgc gtgaaatctt cttctacaat gaatctctta
    15781 tctccaggaa tcgcttatag cgatcaatta acgactcaaa tcgtcaacga aaacaaagaa
    15841 tcagaaatcg tcaaagctca aagagacagt ggtgtaaatg tcttaactac tgaaaaagtt
    15901 gacgaaaatg ccaaacaaac attgcttagt tatttaggtg gagacagctt gccttcaagt
    15961 atcatgattt atccgaacaa ttttgaagac aaagaaaaga tcttggatta tctagatgat
    16021 tacaataaag gcaaatcaga tgaagataaa atcatttata ctgatttagc tggaacgatg
    16081 actgaactga ctggcggatt gatggatgct atcacttatg tccttattgc ttttgcaggg
    16141 atctctttag taacaagtat gatcatgatc agtatcatta cttatacatc tgtgattgaa
    16201 cggacaaaag aaatcggtgt actaaaagct ttaggtgcac gtaaaaaaga catcactcgc
    16261 gtatttgacg cagaaacgtg tattttaggt atttcttctg ggatattagg tgtgtttatt
    16321 gcatggctag ctacgttccc tatcaatagc atcttataca acatgacgga tctaaaaaat
    16381 gttgctcaac taaatcctgt ccatgcgatt attctagtga tcgtctctac tatattgacg
    16441 atgcttggcg gtcatcttcc tgcacgaatg gcagcgaaaa aagacgccgc tatcgctttg
    16501 cgtgcagaat aaggttgtga caaccacatt tagatttgag tcataagagc gctgaaatag
    16561 tagaaaacag caagaaacga ggaagtaata tgttccttgt ttcgcgctgt tttttttaat
    16621 tattttttat aattctggta tacttttctt tcctttgtac cacaactctt ctggcgtata
    16681 cctgtctttt ccatttatga atactgttcc atctgcttca atcgttactt cagtcccaat
    16741 ccacatatta cgagaaagtg cgagaagatt ttttcttttt cctcttcctt tttttccaaa
    16801 ttgcaaggca tcggttatct cttctgtcca atgagatttc acgtatttta cttctccgtt
    16861 ttcaaatgta actttcaaaa tattcgctat attttccact tttactgctt taggaaatga
    16921 ctcctccatt tttttcactt tccttcctcc aacactaagt atgcgctttc ttttagtgat
    16981 aagtatatca tagtttattc tattatcaaa aaaacgcatc cattccattg tagaatactt
    17041 taccgaacaa gttgaattgt tttcctcttt ttcacctttt actatagata ggtaaagaca
    17101 actaaggagg aatctctcat gacagatcaa ccgtatgatt taagaaaagt catcgaagaa
    17161 ctaaaaacac ttccaggtca atatcacgaa acagatattg aaatcgatcc tgaagcagaa
    17221 ttatctggtg tttaccgtta tatcggtgct ggcggtacag taaaacgtcc gactcaagaa
    17281 ggaccagcta tgactttcaa caatatcaaa ggtttccccg gcacacgtgt gaacatcgga
    17341 acaatggcta gtcgtaaacg tgtaggtgaa attttgcacc atgattatcg aacacttggt
    17401 cgtttcttga aagacgctgt agaaaatcca atcaaacctg tgaaagtaga caaagcagac
    17461 gctccagcac aagaagtcgt tcatctagct accgatgcag acttcgatat tcgcaacatt
    17521 ttgcctgcac ctacaaatac tcctgaagat gctggtccat acatcacaat gggtgtagtc
    17581 ttaggatcag atccagaaaa aacaatgagc gatgtaacga tccatcgtat ggttctagaa
    17641 gataaagata cgatcggtat gtacatcatg cctggcggac gccacattgg tcacttccaa
    17701 aaacaattcg aagccttgaa taaaccaatg cctatcacta tcaatattgg tttagatcca
    17761 gcaattgcca ttggcacgac tttcgaacca cctaccactc cacttggcta caatgaacta
    17821 tggtgtgcag gagctttgcg taacgaacct attcaaatcg ttgatggtat agcagtcgat
    17881 gaagtgggta ttgctcgtgc tgaattcatc attgaagcag aaatcatgcc aaatgaaaca
    17941 atgcaagaag atatcaacac gaactcagga aaagcaatgc cagaatttcc tggctatact
    18001 ggggatgcta atccagctgt acaagtagtg aaagtcaaag cagttactca ccgaaaagac
    18061 aatccaatca tgcaaacaac gattggtccc agtgaagaac acgtatctat ggccggtatt
    18121 cctacagaag caagtattct tgccttggtc gataaagcta ttccaggaaa agtagtaaat
    18181 gtctacaatc ctcctgcagg tggtggtaaa ttgatgacca tcatgcaaat ccgtaaagaa
    18241 aacgaagcgg atgaaggtat tcagcgtcaa gcagctattc ttgctttatc agccttcaaa
    18301 gaattgaaga cagtcatttt agtcgatgaa gatgttgata ttttcgacat gaacgatgta
    18361 gtttggactt tcaacactcg tttccaagga gataaagata tcatcgtgat cccaggaatg
    18421 aggaaccatc cattggaccc atcagaatta ccagaatata atcctggctc tattcgtttc
    18481 cgtgggataa gtgcaaaaac tatctttgac ggtacagttc ctttcgatat gaaagaacat
    18541 tttgtacgtg caaaattcca agaagtagaa gattgggaaa aatatttgaa ataatgaatg
    18601 atctgcaact gttttgacat cataaaaagg agtgcggaaa atggaacaat tggcacaacg
    18661 gaagaaaaaa atcgtgattg gtgtaagtgg cgcttcaggc accatttatg ccatcaacct
    18721 agtgaagaaa ctaaaagaat atccgatgat agaaacacac ggaatcatca gtccatgggc
    18781 aaaacagaac ttaaagctgg aatccgactt atcactaact gaattcgaaa gcctgtttga
    18841 ttaccattat tcaaacaaag atttaggtgc tgctgtcgcc agcggatcat ttttgaccga
    18901 tggaatgatc atcgtaccgg caagtatgaa aacagtcgca ggtatctctg ttggtttagg
    18961 cgacaacctg attgcacgtg ctgctgatgt aacaatgaag gagcaacgaa agttagtcat
    19021 tgttcctcgt gaaacgccat taaatgccat tcacttggaa aacttgacta aattagcacg
    19081 actaggtgtg caaatcatac caccaattcc tgctttttat aatcagcctc aaacgatcca
    19141 agatcttgtt aatcaccata caatgaaact tttggatcaa tttgaaattg taaatgagca
    19201 ttcaaaacgt tggcagggtg attaatatgg aaaaagaaac gatagttgaa gtaaccaaac
    19261 taggtttcac gatccaagct gctgttaaag taatcggtaa agacttattg atcactttaa
    19321 caggcggaga tacaccacat atcggaacag tcactacatt ttctcgtgat acagagatgc
    19381 aaactgtacg tttccctagc cacgatggtc ggttccataa agacgatgtc ttagccgaaa
    19441 aaatagctgt gatcatcaaa actgccgttc cgggaagttg tacgatcact tccggtatcc
    19501 atgttgacca tatctccaat gcacaaatca acgcctcttt tccgatgtca gaagagttag
    19561 gttacaagat tttagattgg ttggaaaaaa tagatttcca tatcaaataa ccagtttatt
    19621 ataaagatgg tgaaaaacca ttatgaaaga agaggttgtg aaatcagcgt tatgacctga
    19681 gtatgagaga aaaaaatgga aaaatagctt ctcatatttt gccgtttttg agctaatacc
    19741 gagggacagc tgattgaacg ccgtttatca agaggttgtg ataaagccgc ttcgtttcga
    19801 gtagtaagaa aaattttgta aacatgggtt tccacatttt tgcataaatt tgacttacta
    19861 ccgagaaact ggcttttgaa caccgtttat caagaggttg tgacacgtct tttgtcacaa
    19921 cctcttttat ttttgcttca ctgtaactgc tacaatatca ccgatttctt ttccgatgtt
    19981 tttacgaata tcttttcgta gtccaaggat gtagcagact gaaccatctg gatttttcac
    20041 gcccatattc acgatactgc cttcatacgg cacattgtca aaagttgctt ggactttaac
    20101 tcttccagca ccgaattctt ttcttatatc atacggaaaa ataatatatg ccccgccctt
    20161 gccgacttcg gaagcataga tttttgattc aaaagaataa atttccctca ttttatttct
    20221 cctatttttt acatctagta tagcatagca aaaaaaagaa ggaactgtga caaaagtctt
    20281 gtcgcagttc cttttttcga ataaacggtg ttctcctgcc aactcctcgg tcacaagtca
    20341 caatagtcaa caaacatgag aagctgtttt ttgaaattgc tccttgtgac tcagagttaa
    20401 acggcaggct cacaacctct tccttaaacg gtgttcaaaa gcttgcatct acggcactaa
    20461 gcccaatcag atgaaaaata tggagtgcta tttttatctg attgttctta ctgcttgatg
    20521 cagagcagct ttttcacaac ctcctacatg gtgttcaaaa tattattcgc cttttgcttc
    20581 tttcactaat tttgcgaatg cttgttgttg gaattcagca gctaaggctt tcgtacgttg
    20641 ttcttgctct gctttgctca tttttggatt ttcagtcatt gcttttacca taccgtcttg
    20701 aacataagct tttgcttcac tttccatcgt actttgctgt ttcttcacta attcagctaa
    20761 cttggctgct tggtccgtac ttaattctac ccatgtctct gggattttga atgtattcac
    20821 tcggctgtca tattcgtgat tgttatcagc aatattgaac cagaatttat acatgtcgtt
    20881 tttgtattcc caataagtag ttttcgtcac tttttcagca tcgccgtctg tcttttctac
    20941 tttatttgtt tcgttatccg taccagttgc ttttggtttt tcttgttttt catttgttcg
    21001 gtaaagatac actttttctt ttccattccc taaagcttta tacaaaagca tatctgctcc
    21061 ttgggaatca gcactggaaa caattttttc tgtttttgtt tccgttactt tttccattcc
    21121 aaagtgattg gacaaattag cgacgatcaa acttagtgaa gcgacgaaga ttagtccgaa
    21181 aatcaaagat aaaaaggttt gccatgtttt tttagcgaac acgttcgtta aagcaaaagc
    21241 aagcaagctg acaatcaaaa ctccaataat catgcattat cccccgcttt cattagaaca
    21301 ggttcttttt tcttcaataa aaaggcaata ataaagccaa gaatcccaaa gatgatcgct
    21361 acgataaacg cggcatggta acctgacagt gtggcattga ttgcctgatc tttataagct
    21421 aatggcgtat tatccaatac agatttgctt gggatatttg cttttgtcac attcgtcaac
    21481 acactgatca aaatagctgt accaatcgag cttgcgattt gacggaatgt attatttacc
    21541 gctgtcccat gactgatcaa gttatttggc aatgcattca taccagaagt cgttaccggc
    21601 atcatgacca tggagatccc aaacatacgg atcgcataaa ggatcacgat atataaaact
    21661 ggagtcgttt ttgtcaagaa agcaaatggt gcagttgccg ctgtcaaaat gaacatccct
    21721 gtgatggcta aacgtttcgc gccgtattta tcgaaaatcg cacccgtcac aggcatcata
    21781 gcccccatga tcaatgcacc cggcaacaac atcaagccag aatggaatgc tgattctcca
    21841 cgaatatttt gaatatacaa tggtacgacc atttctgccc ctaccatcgc catatttgtt
    21901 acaccagcta aaacagctgc aatcgtgaat gtagatgatt tgaatacacg aagttccaag
    21961 aatggatgtt ccaaatgtaa ttgacgccat gagaacaagg cgatgaaaat cacaccaaca
    22021 actaagaaac caattacttt caagcttccc cagccgtcat ctcctacact agaaaatccg
    22081 taaagtaaac taccgaatcc aatcgttgat aaaatagcgg ataaaatatc aatgctagga
    22141 tttgacaatg gaataacact tttcattagg aagaatgcta aaaccaagac aagaacaacg
    22201 attggcagta ccataccaaa caagtcacgc caatggtagt tatcaatgat ataaccagat
    22261 aatgtagggc ctaaagcagg agcaagtcca ataacgatcc ctgacatccc cattgctgat
    22321 cctctttttt ctggcgggaa aatagatagc atgatatttt gcaataatgg catcgatact
    22381 cccacaccgc aagcttggat caaacgaccg attagtaatg tactaaaatt aggtgctgta
    22441 aagcaagtaa tcgttccgat caaaaagatc gtcatggcag aaagatataa atttcttgaa
    22501 ctgaatttat tgatcagcca tgctgttatt gggatcatga tcccgttgac tagcaagaaa
    22561 ccagtcgtta accattgcac atcagacgct gaaatatcga aagctttcat caatgtagga
    22621 aaagctgtcg tcaataaggt ctgattcaaa acggtacaaa atgtaccaat taataagaca
    22681 acaactaaaa gtgtccgatt atatggtttt ccgtaaatat ctacgggttg gttgttactc
    22741 atatttctcc ttctttctga attttttctg aaactactag aatactttag tcctaaattc
    22801 gctatttgtc aacctacttg acacataagt taatacggat ataattaagg gaagcaagag
    22861 gacaaaagga gtgacaaaaa tggttggggc ttcccttaaa cgtttgttgg aaaatgatca
    22921 attggttgtt ggcatcagtg aattaagtga tatcgtaggt gtgtctccac gtcaattaag
    22981 gtattgggaa caaaagggat tcattcgttc gatcgaagct gatgcgaatt gtcctagaaa
    23041 atatcgttta cctacagtca taaaagtaga actgatcaaa aaatatttag atgacggatt
    23101 tactttgtca aaagctgcag aaaaggcaga aatccgccaa aagaaaatgc accatgtccg
    23161 taaagttttt tcaaaagcaa tcaaagatat cgaattgatc gacgaacgct atacgatttt
    23221 acaaatcggt aacttcaaag aagagaatgc taagatgtac atcattcatg acaacgagac
    23281 agacgaatta acttacaagc tccttcctgt cgatgaaaaa gctgactacg aacagttgtt
    23341 aaaagaactc taaacgccac taatctccta agtaccaagc tttttaataa gtagatgcta
    23401 cataaatact tgaaaaaagg cctgtttgaa cataagaata tccgaacggt gatctgcgtt
    23461 tctttcatat agaaaggaat tctccatcac tgttcggata tttttttatt tttttcggtt
    23521 actttcttct aatacatctg ttagccaatt tgcatattct gtttcccgat tgatcgcatg
    23581 cgctaaaatc aggtagtgtc cgtaattttc atgtttttct ttttcagtag gaaagatcgt
    23641 tttttttcgc tctaatagat gaagtcgttt ttcttcatga aggtcacgct gctgctgaat
    23701 aatttctact aatgccggat cattcacgtc tttgacgaaa tacaatttca gaacaaattc
    23761 gtctcgatta accggtagtt cattggtggg tgtatgaatc cactctgcca attgttcctt
    23821 cccttcttct gttaattcat aaactttctt ttccaatttt tcacctgtga tctctacttg
    23881 atgctgaatg attccttgct cttccatttt tgcaagttca ggataaatct gactatgttt
    23941 tgcttgccaa aattctccta tttcattttt gaacgattga ttcaattcat atcctgtcat
    24001 cttcttctta cttaataacc ctaacaaaat atatcgaagt gtattttttc ttgccatcag
    24061 ttttccctcc ttgacaaatc tttaaatcca ttctatcata aatgagtaat cgaaaacatg
    24121 tagtttatta catgttttaa tttataagga gatgcttaaa catgaaagaa tttaaagaat
    24181 tggaagattt tattggaact cactttatct atacgtatga caatggttgg gaatatgaat
    24241 ggtatgccaa aaatgaccat actgttgatt accgtatcca tggcggaatg gtcaaaggcc
    24301 gttgggtaaa agaccaagaa gctgatatcg ttaaattgac ggacggcgta ttcaaaatca
    24361 cttggacaga gccaacaggg acagacgtgg cattggactt tatgccaaat gaaaagaaac
    24421 ttcacggaat gatctttttc cctaaatggg ttgaagaaca tccagaaatc actgttactt
    24481 tccaaaacga acatatcgca gaaatggaag cagctcgtga aaaatatgaa acttatccaa
    24541 aattacttgt tcctgaattt gctacgatca cttatatggg tgatgcaggt atagacaatg
    24601 aagatgttat tagcgaagaa ccatacgatg gaatgccaga tgatatccgt agcggaaaat
    24661 acttcgatga aaattataaa cgagtaaaat aaggttgtga aagagaggtt gtgaaatcag
    24721 cgttctgacc tgagtattag agaaagaaac ggaaaaatag ttcctcatgt tttgccgttt
    24781 tttgagctaa taccgagggt cagctgattg aacaccgttt atcaagaggt tgtgaaatca
    24841 gcgttatgct ttgagtaata agaacgctag ttagaaaata gcttttcata ttttctaggc
    24901 tagtgggctt attaccgaga agcagccgtt tgaacaccgt ttatcaagag gttgtgaaat
    24961 cagcgttttg acctgagtat tagagaaaca aatggaaaaa tagttcctca tattttgccg
    25021 ttttttgagc taataccgag ggtcagctga ttgaacaccg tttatcaaag attgtaacaa
    25081 ctgcatattc acacagttaa attagtgact tcaaaatgaa ataatgctcc cagcaaaaaa
    25141 ttaggctggg agcattattt cgttattctg ttcatcaaaa atcagcgttt tgattccatt
    25201 tcatcacaag ttcctcctca tttactgctt catctttttg ctgtgtttga attgtaaaac
    25261 cttgtgaaag atagaaatga atagctgatt gatttttttg atagactgtc aaagtcagtg
    25321 ttttattggt ttcttttatt tttttcatta gttgcgtacc gattctttgt tgccggtatt
    25381 cccttttcac aaagattcct gcgataaact gatcaatcac cccagcaaaa ccaataattt
    25441 cttgatctat ttctgctaag taaatcactg catctggtaa agctttccta acagattcta
    25501 cttgattctc ccaataagtt gatggaatga attcatgaga atccaagtta gcatgcagcc
    25561 aaatcgatag aacagattcc agttctttgg cagacaattt tgttgttgtg cggatcattt
    25621 tttctctcct tacttattca ttgattttaa aataaaaacc atttttttga agctctttta
    25681 caagttttcg cccctcaaaa aagtatatct tctcttgttt tacttttcta ataccaaaag
    25741 aaacagcaac tttaggatca tcagctgaaa cgattcgata agttccacca ggcaattgat
    25801 ccagcttgac tttcccatga tggtcgcttt gtactgaaaa cggcacacca tttctagtaa
    25861 gtgttttttt cttatgtttc aaaatgaatg aactaccatt taccggtttc cctgacgaat
    25921 cactaataat aaatgaaaaa tcaagcactt ttttacttaa tctatactga tagattagtc
    25981 gtccgataaa cactaacagt atgactacag ctattatcgt tcctgcaaga ataaaccatt
    26041 gccgcatggt tctttcctga tttccttttc cttcttcttt ttttaccgat tcgttataag
    26101 gaatccgatg tcctgtcact agcaaacgat gcgtgttgat catatacggc gtacatgtaa
    26161 gcaaggtaac taagtcttct ccttctgaaa tcgttagaac agacgtgtca tctggcagta
    26221 ccacttgtat atttttaact tgataagcca atttctcccc caaaacagtc aaaataaacg
    26281 tgtctccgtt ttctaaacga tctaattcgg taaataaccg tcgattaggt aaaccgctgt
    26341 gagcagagat cactgaatgc gtcgaatttc caccaagcgg gaaagaggtt ccttgcaaga
    26401 cgcctgctcc attttcaagg atcctaccat tgagccgatc ataaagtgaa atatttaatt
    26461 ggatcttagg tatattgata cttccgatca gccattcctc taattggctg gatgttgtac
    26521 tttcatttct gttcttttca ttgaaaggat cagtaccagg attgaatcct tggtcagtta
    26581 atcgttggtt tttctcctcc attttctttc gctgcacttc aatttcagct gcggtccgtt
    26641 tttgtacttg ttcgatacgt ttttgatcaa taaaactatt taaagaatcc acataaaatg
    26701 gatataaagc gatcagtaac cctacgacga tacatattaa taaaaacaaa cgaaacaaaa
    26761 gtcgacgaga ctgtttttgc ttactctgct tcacatgtgt cctcccttgc aagttttttt
    26821 gttctttctt ttattatatg ggttcaggaa aaaaatagaa aatgaaagcc gatgaggttg
    26881 tgaaaaagct gtcctgcatc aagtaataag tacagcccaa aaaaatagct actcatattt
    26941 tttgttgggt tgggcttatt accgcagatg caagcttttg aacaccgttt aaggaagagg
    27001 ttgtgaaaac tgcgttatgc tttgagtaat aagagcgcta gtcagaaaat agcttctcat
    27061 attttctagg ctagtgagct tattaccgag aagcagctga ttgaacaccg tttaccgaga
    27121 ggtcatgaaa aagctacttt gtttcgagta ttaagaaaaa ttctgtttca aaacaaggag
    27181 atgcaacaaa acttatgtcg catcctctct ccttagaact ttcacttatg tttttcttgt
    27241 aaaattcata aatattttca tgaattttct aatttcttgc ctacctctcg cataggatat
    27301 atgatgaatt tatctaaagt ttgcaaagga gttcgacaat tgaaaaaata tggcccattt
    27361 ttagcaattt ttttcattgc tttggcattt ctcatgcctc atttattcac acataaacta
    27421 attttgggtg ctgatgcccc attccattac aatcggttct atgatgccgc gcaacaaatt
    27481 aaatatgggg atttccatta ttttccttcc ctttatggat tccagcaaag tggcaggatc
    27541 gtgaatgccg tttacggccc atttttcgct tatttacaag gattgatcgt gttgcttgca
    27601 ggtagctggt ttcattatca gattctttca aatttcattc tttatcttat ttcaggattt
    27661 tctatgctga ttttactgag atatgtcaat gttcgaaatt gggtaagtgt accagtagcc
    27721 gttttattca tgagtacgta ttctattcaa tactggtcta tcaaccaagg attcactagc
    27781 tgggggactt gtttttatcc tttatgtatg atccctattg ctgatttcgt ccttcataaa
    27841 aagttccctg ttatcaaagt cgctgtctca gtgggactta tgacacagat ccatttactg
    27901 tcttcgatca tgttagcttt gatgtatatc cctttttatc tttattactt ttttaaccag
    27961 actgaaaaag gaaaagcact gcttcaactg attaagtcgg tcttgcttta ttttgttctg
    28021 acagcaaata tttgggcagg attagctatt gtttacagtg ggaataagat tcttcctccc
    28081 tttgttaaca gagaaatgag tcaaaaggcg atagatctgg atggcagtta ttggctccat
    28141 taccctaagg cctttcctat attgatatta tcaggttttt tgctttttat tttctttcaa
    28201 caaaagaaca ccttattcca aaaaatcgtg ttgtacacaa gtcttgtttt cttttttctt
    28261 tctactagct tgattccatg gacgacattg atcgaaaaaa atgtgccttt tattagtttg
    28321 atccagtttc catttcgatt tttcgtgcca tttacgctat tgcttctact ttaccttgcc
    28381 ctttcactga accagtggtc tgaacaaaag tggttccgtc ttttaagtat atttggcatt
    28441 cttatttttt cagcacagac gatccagaac ttataccagc atctggaaaa gtgggaaaat
    28501 gaaacatttg tcagtcgaca tacttatcta ttcgatacgc cggaagaagc aaggaaaaca
    28561 ttcttcagca aagatatgtc taccagcttg ttcgttttcc aaaaaaccac acctgactat
    28621 ctgccaatct ataaggagac aaaagacaat aaatacgacc ggtataacga agaaatacta
    28681 gaaaacgaaa caacttttat aaaaacacat ccaggcggtc ttttgacaat caagtggaaa
    28741 aacactgggg acaaaactgt tcatatccct gttatcgtct acgatcggac tgtgctacag
    28801 caaaatggaa aaacattgac tgattatgaa gtaacagata tcgggacccc aatcgtgaag
    28861 cagcaaaaag gaatcaacga gctcacgctt cattatcaaa cgccaatcta tttctatttc
    28921 attctttctc ttactttgat tggctggttt accttgctat gtttatttat ttatcataga
    28981 tacaagcttt tacgtgctta ggcaaaaggg ataaatacat ccgatcaaaa aactctccaa
    29041 acggtataat cgtttggaga gttttatttt aatcagtttt tttatgttat atgttatatt
    29101 ttttattcgt tcacatctaa ttcgatttcc accccgtagt gatcactgat gattggacta
    29161 tcgatcccat caaaaatccg ttgatatttt ttaacctgca ttccttcagg gacgaaaata
    29221 aaatctatac gcaatttttc tgtattttgt tcccaaccat ctattttctt ttccacagta
    29281 gctgcttttc ctttttcatt cgcgacaaca tacgaatctt gaagaagaag gcaactttct
    29341 cttacagtat aataaccttg agagtctact ggattattga aatcacctag gaaaaaaagt
    29401 ggtacgcgag tttccaaaaa gtagttttcc aagtttttcc actcaaaata aaagccactt
    29461 tttttatctg tccaccaaga aaagtggcag ctcgagacaa aaatgtcctg atcatctatc
    29521 tttgttttcc caatcaagat cttgcgtgtc tgataatctt ctttcttttg aaattgggag
    29581 acggtaagga cctgactttc caatgttttt ttcgtaagca gtgcatttcc ttcttcatat
    29641 ttatcataac cgatgtggct catttcccaa ctccaaaaat actcttttcc gtgttccttt
    29701 aaaagctgaa ctaaacggta ggcaaaatta tctttacgga taggtacttg attctttacc
    29761 ggacagaact tcattagctt cgcaggttct acttcttcag attccagtaa ttggttgatt
    29821 tcttgtaaag caatcacttc ataatcttct aacaggatct tatctgctaa ttcttgcaat
    29881 tttttctctg gttctggctc aagccagcta tgcgtgttga gtgtcatgaa tttcattggt
    29941 tttctccttg tcttcatttc gaaacaaaag ggttgtgaca aaatttttgt cacaacctct
    30001 ccagcaaata gtgttctcct gctaactcct cagtaataag ctcaatggac caggagctat
    30061 tttttgtttg gctgttttta tcacttggtg cagggcagct ttttcacgac ctctccacta
    30121 aacagtattc aaactgctgc ttctcggtaa taagcccaac caaacgaaaa ataggaggag
    30181 ctatttttgt ttggctgttc ttatgacttg atgcaggaca gctttttcac aacctctcct
    30241 tacattttaa tggagccgat cactttacca gctgtcactg tacctaattg cttgatcact
    30301 acttcttcta ctttatcgtt tgtaaatacg atcatgatcg ttggatcttt ttcttcgttt
    30361 tggatcacat caagatccat ctcagctaat ttcgttcctg ttcctacttc ttggccttct
    30421 ttgacagcaa tctcaaaagc tggctgattc atttggactg tatcgattcc catatggatc
    30481 aaaacttcga taccatcttt cgtttccagc ccaattgcat gtttgctagg gaaaatactt
    30541 gtcacttttc cagcgatcgg cgcaacaact tctcttgatg ttggaataac agcaaagccg
    30601 tcacccatca ttttttgtga gaataccgga tcttttactt cagataaagg aatcacttct
    30661 ccagaagctg cggaaactag ttcttgttct atacctaagt attgcttact acctgcttgt
    30721 ccatttcctg tagaaaccat ttctggctct ggaatctctg ctcctgattg cagcagatct
    30781 tcgatatctg atttcaagac atctgctttt ggtccataaa ctgcttggac tccattgtct
    30841 ttgacgatca atcccatcgc ccctgctcgt ttccaggctt cttcagtccc tactttttct
    30901 cgatcattta cacttacgcg taaccgagtc atacaagcat cgacatcttt gatattttct
    30961 tttcctccca gcaaatgaat gatctggacc acttgctgat ctgctgttcc tgttccgctg
    31021 ttagatacaa cggtttcttc actgtcattg tcgtaatttc cgttacgtcc cggtgtagca
    31081 aagttgaatt ttttgatcaa gaaatttgct aagaaatacg taacaacacc aaaagcgatc
    31141 acacacaaca cgaagttgat aagatctcca cctagacccg ctttgatagc taacggtgtc
    31201 cgagtcagca attcgatatt cccaaatgaa tggacccgca atggcaagat atctgccatc
    31261 gcaaatgcag ccccttggat cactgaataa atgacatata atggcacagc agcaaacata
    31321 aacatgaact ccaatggttc tgttacacct gttagaaaga cagccaacgc tgctgaaaaa
    31381 tacattgatt tatatcttgc tttcttatca ggatcgacat tacgatacat cgcaaaagcc
    31441 attcccatca agatacctga agaaccaatc atttgtccta ctttgaaacg agccggcgtc
    31501 cagttatgca acacaaactc atattgcgac atatcccctg cacctttaag attcactaaa
    31561 tcagtggccc aagccaacca taacggatct tgcccaaata cttgtgtccc cgcctgtgcg
    31621 cctgataaaa tctcatacga tccgccaagc tgtgtatagt tgataggaat cgtcagcata
    31681 tgatgcaatc caaatggcaa tagtaaacgt tctaatgttc caaataaaaa cggtgctaga
    31741 atcggtgcgc tttcttgtga ttctgcaatc cataaaccaa aattattgat tccagcctgt
    31801 acgtttggcc acaccacagc aagaatcaat gccacgattg ttgaccataa gataacaaca
    31861 aacggaacaa agcgctttcc attgaaaaat gacaaagcat ccggcaattt tctataatta
    31921 tagtacttat tataagccat tgccccgaca aatccagcga tgatccctac gaatactccc
    31981 atattcaaag ctggtgcttc caatacactt gtaaaaaatc ctttaaccat gatcttcgta
    32041 ccgaacagag tgtgggtaaa tgcatcttca ttcgcaagca tgtcacttgt gacaccaaag
    32101 atagccccag tgatccgatt gattaaaata aatgagatcc cagctgcaaa tgctccgcct
    32161 gctctttctt ttgcccaact tcctcctatt gctaaagcaa ataaaatatg aagattacca
    32221 ataattgccc aaccaatatt ttcaattact ccgcccgttg tgacaagcgg cgtccaatca
    32281 ggattgatca agggaattga tttccctata ctgatcatca acccagctgc cggcataact
    32341 gctaccacaa ccattaaagc ctttccaaat ttttgccaaa actcaaaact aaatatcttt
    32401 ttcattccgt ttagctcctt tacgcaatcg gtttcgttaa acgtattata gcgtgcgttt
    32461 tcttttttgt aaatgcttta ttttttattt ttctgtctat accattccat aaaacctata
    32521 atacgtgtgt tcccttctat aattttctcc taaaaattta tttttttaag cttttttcta
    32581 tgaaatattt tcttttgtct attgacttgt gcaatcggtt gcgtttatta tgaacgtata
    32641 aaacagctca ttaggaggag aaaaatgaaa cagatcaaac gactatttgc tattgatcca
    32701 tggaaaatca gcacgacaca attagataaa aaaaatcttc gactgcaaga atctttaaca
    32761 agtattggaa acggttatat ggggatgcgc gggaactttg aagaaactta ctcaggggac
    32821 caccatcaag gtacttattt ggcgggtgtc tggtatcccg ataagacacg tgtcggctgg
    32881 tggaaaaatg gttatccaga atactttgga aaagtgatca acgccttgaa ctttatcgcg
    32941 atagatattt cgatcaacga tcagctgatc gacttggctt ctttggaacc agaagacttt
    33001 tactgggaat tagatatgaa aaatggtgta ttatctcgta cattcacaat cactacttct
    33061 actaataaag tacgcatttc cttcgaacgt tttctgagca ttgtcaaaaa agaatcagct
    33121 tatatctatt taactataga aatgcttgaa ggtagcggaa cagtcaaact cgtatcaaaa
    33181 ttggatggaa atgttcaaaa cgaagacagc aactatgatg aacatttttg gaatgagatt
    33241 gatcgtggaa ctatagaaga aatcggctat ttgacgacta aaactattcc aaacaacttt
    33301 gggattgaac aatttactgt tactgcagca atgcgccatt tcgttaatgg aaaatcagtc
    33361 atccctgact atcacgaaga tactttagca ttacatggtt gcttgtctgc agaattatct
    33421 caaggtgaga catggtcagt ggaaaaagaa attatcgttc ttactagtag agatattcca
    33481 gaagcgcagc aagcagaaaa agctgtctca gaattaacga accagacaaa caattatatc
    33541 caagcaaaag aagagcaaac agcggcttgg gcagaacgat ggaagcttgc cgatgtggtc
    33601 atcgaaggag acgaagaagc acaacaaggt attcgattca atttgttcca actattctct
    33661 acttactacg gcgaagatga acgattgaat atcggcccca aaggattcac aggagaaaaa
    33721 tacggcggag ccacttactg ggatacagaa gcgtatgctg tccctcttta tcttgcttta
    33781 gccaaacctg aagtaactaa gaatctgctg aaatatcggc ataatcaact gccgcaagca
    33841 atccacaacg ctcagcaaca aggattaaaa ggcgcgcttt atcctatggt caccttcact
    33901 ggtgtcgaat gccataacga gtgggaaatc acattcgagg agattcaccg aaatggcgcg
    33961 attgcctatg caatctacaa ttatacgaat tacaccggcg atacggaata tttgaaaaat
    34021 gaaggactgg aagttttagc agaaatcgca cgcttctggg cagaccgtgt ccatttttca
    34081 aaacgagcaa ataaatacat gatccacggt gtgaccggcc caaacgaata tgagaacaat
    34141 atcaataaca attggtatac aaatacgatc gcagcttggg tcttgcgtta cacgcgagaa
    34201 aattatttaa cttatcaaaa agaagctgct atcactatca gcgaagaaga actagccaaa
    34261 tggaaagata tcgcagagaa catgtacttc ccagttgacg aagaattagg aatctttgtc
    34321 cagcatgaca cattcttaga taaagatctc atgcctgtat ccgatattcc cacaagcgat
    34381 cttcctctga atcagcattg gtcttgggac aaaatcctta gatcttgctt catcaagcag
    34441 gcagatgtac tacaggggat ttatttcttc aatgatactt ttacaaaaga agaaaagcaa
    34501 aaaaactttg atttctatga accaatgact gtccatgagt cttccctttc gccaagtatc
    34561 cattcgattt tagcagcaga attaggaatg gaaagcaaag ctgtagaaat gtaccagcga
    34621 acagctcgct tggatttgga taattacaat aacgacacag aagacggctt gcacatcact
    34681 tccatgactg gtagctggtt gtcgattgtc caaggatttg ctcaaatgaa aactgcccat
    34741 gatcagttga gattcgcacc atttctgcca aaaacttgga aagcttattc tttccatatc
    34801 aattatcgcg gacgtttatt gttcatctca gtcacagatg aacaagttac tttcgagtta
    34861 ttagaaggtt ctcctctaga aatttacgtc tatgaacagt cttattcttt agcggatcgt
    34921 ctagaaataa cgctaagaaa ggaggaagca aatgtttaaa ggggtattgt ttgatttaga
    34981 cggtgtcatt accgatactg ctgaattcca ttatcgtgct tggcgaaagc ttggtcagga
    35041 aatcggcatt acgatcgacc acacattcaa cgaacagcta aaaggcgtca gccgagaaga
    35101 ctcactctct ttgcttttag cttatggcgg aaaagaacac tctttttcaa aggaagaatt
    35161 tgcagaatta gcgaaaagaa aaaacgatta ttatttagag atgatccaga cgatcgaacc
    35221 aaaagatgtc ttccccgggg tcgtgccttt actggattct ttaaaagaag aaaaaatcaa
    35281 gattgctctt gcttctgcaa gtaaaaacgg gccattccta cttgaaaaaa tgggtcttac
    35341 accttacttt gatgctattg ccgacccggc agaagcaaca aacggaaaac ctgcgccaga
    35401 tatcttcctc cttgcagcaa aagctgtcgg actaaaagca gaagaatgta tcgggatcga
    35461 agacgcgcaa gccggtatcc aagccatcct ctcaagtggc gctcaaccaa ttggcgtggg
    35521 acgtgctgaa gaccttggag aggagatccc gcttgtccct gatacgactt ttcttacgat
    35581 cgattacttg aaagaaaagt ggcatgacca tggataatcc agagatctcc gtcgtaccag
    35641 ttccagagac tgaaataaag aaaatcacat tgaagaacga ctacttagaa gtcgttcttc
    35701 tcaattacgg tgcccgcctg caccaaatct tcgcaccaga caaagaagga aaatcagaaa
    35761 atgttttatt gtcttacgat cgtttcgaag atgttctaac agacaaaagc ttttttggag
    35821 caactgtcgg acctgtagcc ggaaggatcc gggatgcttc ttggggtgag catagattgg
    35881 agaaaaactg cggatcacac catatccacg gtggcacgaa aggctggtct ttccaatttt
    35941 gggaagtaga agttttcaaa actccatcat caataggtgt ggtcttttac ttaaaagatg
    36001 aattttctac ctatccagga ccaatcactg ctacgatcac ttaccgttta acaaaaaacg
    36061 aattagagat gacaacctct gctagtagcc ttgttgaaac catctgcaat ccgaccaacc
    36121 atgcctactt caacctttca ggaaatggaa caagagacat ctatgaacat cagttaacgg
    36181 tctttttaga tggtgtgctt gaattagatc aagaaaaact gcctactgga aattggacaa
    36241 aaaaagaaaa actgccaatc gactttagaa aatccccaac actgcaagag atccttgcat
    36301 gttatccaga tggactagat gatgtatttc ttttgcatca tcctaggctt tccaaaactt
    36361 ccttacaatt attcgagaag cattctggtc gccaaatgac tatagcaact tctaataaaa
    36421 gtatggtatt attttctacg actggattcg aagctgattt ctctgtcaat ggaaaacaga
    36481 tgcattcaaa ctacggatta gcaatcgaac cgcaagaaat ccctgatatc gttcatttcc
    36541 ccaaatttgg atcgatcaac ctccatcctg gtcaagaacg gatcagtcag acgatttatc
    36601 ggttctctgc acaataacaa gagaaaggtg tttttatcat ggctattacc gtaaaagatg
    36661 tggcgaaaaa agccggtgtt gccacctcga ccgtctctcg tgtcatcaac gatcatccta
    36721 gtatttccga gacaacaaag aagaaagtcc gaaaaattat ggatgaacta ggttatgttc
    36781 ctaacatgac tgcccgcaat ctaggaaaac gcatttccag tgccatcggc gtaatcttac
    36841 cccctcttga ttcaaaagag cggatcggta acccatttta tcttgagatc atggaagcaa
    36901 tcaacgaaga agcgcggaat tatgatatga ctacagcaat cgctactgct aaaaattttg
    36961 aattgctgtt agagaacgtc cagcgtatgc atttacaaaa acaagtagat ggttttattc
    37021 tagtgtattc agatagtcaa gatactgtca ttgattattt ataccaaaac cacatccctt
    37081 tcactttgat cggacagcct tatcatcatg agaacgaaat cgtttatgta gataatgata
    37141 accagcttct aggtaaacaa gcaacagaat ttttgatcca gaatggacat aagaatatcc
    37201 tgtttgtgac aaacacgact catgaaagtt tgtatttcga acgatatttc ggttatcaaa
    37261 aagcaatgat gatggctggt cttcctgcgt tatccagtgt agtactagag catccggagg
    37321 attatctgtc ctttgagaaa acgttgaaag aaaacgaagc cacagcattg gtggtcattg
    37381 acgatatctt cgcccttcgc acgatgcagt tggcgcaaat gtatggctat gtcattccag
    37441 agacactctc cgtcattagc ttcaacaact cgatcttctc aacattaaca cacccttacc
    37501 ttacaagtat cgatatcgat atctccgaat taggacgtgt cgctatgcaa aagctgcgtg
    37561 aattgatcca tgaagaattg agcagtggcg tacgtttggt catccctcat cgcttgatca
    37621 aaagagagac agtcgttcct ttagaaaacc catactaaat agctgactca ctaaatacgc
    37681 tgaccattta tcctatggtc agctttttat gtaaaagaag atactacttc taacacattg
    37741 tttatctgta aaaaagagcg tgggacaaaa gtaaagaaca cttttgtctc acgctctaaa
    37801 cacgtataaa cggcggaagc agaagcaact ccttcggaaa taagctgaaa ttcacaaaaa
    37861 tttaaaaagc aattttcttg agtttcttct tatttcttgg agttaaacgc ttctgtccca
    37921 gcctcttttt tccttccttc aattttttac acgtcgacga ttcaataaaa aaccgcctaa
    37981 tattgtaaag acaggtgcaa gaatcgagaa aaaggcgaat ggcaagtaat gcaacgcgct
    38041 aactttcatc gttcccataa tgaatgtgcc gctgactccc cacggtacga gagaattgac
    38101 ggtcgcacct gaatccgcta gtgttcgtgt caaataggtt tttgataagc caacgtcatc
    38161 aaaagaacgc ttgaatgttt ctcctggaag aatgatggac agatactgtt ctccaacgat
    38221 cagattgatt ccgatacagc tcaaggcagt aaacccgact aaacgagaag gtcggtccat
    38281 cttttctttt aattcttgaa cgatggtatc aacgatcaga tatttgatca gtaacccacc
    38341 taatgccaat gcaagtaaaa tcaaactagc agaacctaac atactcatga tcccgcctcg
    38401 agaaagcagt tcatctagct ttggatcagg tgtatttgcg acataaccat tcatcaagat
    38461 gtctgctgct tttccgaccg tcaaactttt ctcatggata aaagaaagaa cgagagcagc
    38521 tgtcgatccc gctagtaatg tagggatagc cggaattttt ttgattgccc ccccaaataa
    38581 aataactagt ggaatcaatg tccaaggaga gatccaaaag ctcgctttca tgttttggat
    38641 catttcattg atactagcta gatcggctga atgactgtta tgtcctaata tcgtataaaa
    38701 aatcaacgaa aggacgaatg ctgggaaatc cgtataacgc ataccagcga tatgttcgaa
    38761 tagattaact cctccaatac ccgctgccaa gttggtcgta tctgataaag gcgagcagtt
    38821 attccctaaa aaggcaccgg aaacgactgc gcctgtagtc attgcatcat tgaatccaag
    38881 taaatgaccg atccccaaaa aagcaattcc aatcgtcgaa atcgttgtaa atgaactgcc
    38941 gacagtcact ccgacaattg cacaaacaag aaatgctgtc ggtaaaaaga aacgcacaga
    39001 aacaagttta aatccataaa ccataatcgt agggatggtt ccagataaaa tccatgaagc
    39061 aactaagaca ccaatcaaaa gaaaaatcag gattggaatg atccctggag tgattccttt
    39121 gatgatcccg tcatggatct catcccatgt gaacttttta aatctgccat aaaacaacaa
    39181 acacatgaaa gctgcaagga tcgggatatg tggcgagagc tggaatccga tgatcagtac
    39241 tcctaggagt gccaacaaca cgatcaatac agcgatactt tcttttaaag aaatattttt
    39301 attcatcgtc gatctcctat ttcaaatgaa aaggccctgc catgaaaaaa ctattcactt
    39361 ggcaggacct cctactaaat cagcgacaaa acgtcggctg aaagacttat ttttttattt
    39421 ttcttctaaa cttttttcta cctcaatccc ccaagaatct ttcttgaagt gattgatgat
    39481 caatggaata acgaacatga ccactgtgat agctgctact aataatgcat cactcaatgt
    39541 cgctgtacct aatgtagcaa atacgacaac gatgatagag aagatcagca tgcagctgac
    39601 tacccaagct aatccgtttc cgtttttacc aattcggtaa ggacgttcga attctggatg
    39661 tttcttacgc attttaagca atgaaacagc aatcaaaatg tacacgatac aataaataac
    39721 agttgtagca tttgtaagtg tcaagaatac agcacttacg tcatccatta caccataaag
    39781 caaagcaaat aatgaaataa cgattgattg tgttagaacg acatttctag atacgccgta
    39841 tttgttttct ttatggaatc cgaattttgg tggaaggaat ccttcacgag ctacttggat
    39901 gattgttttt gatggtccag taacccaagc tgataattgt aaaagcacac caataaatac
    39961 catgaagctg aagatatttc cgatcacttc tggtaagcct aggatttgac agtcaatcaa
    40021 gataggctgc gtgatatttg ctaattccat ttttccatct ggtacgacat ttgctacaag
    40081 catcgcattg atgacattca ataaaactaa tccgatcaaa gcgataaata cgccttttgt
    40141 ataatttttt gttgcgtctt tcaaacgagg catgtaaaca gaactcattt cgatcccaac
    40201 aaaaataaat gtgataccag ctaagtattt caatgaatca atatgttcca agtcagggaa
    40261 agcttttgac catgaaaagt cacctagata gccgtttgag actagtccga ctttgaaggc
    40321 tgccaaaaca cctaaaacga acatgatgac tactgggata tataccccta gccatacacc
    40381 gatgttcccg ccgacttttg ccatatcgaa tttcaagttc aagatcgtga tgatccagta
    40441 gataactaaa atacaaccta aaacgaacca gtgattattg ccaagttcta cattaccaaa
    40501 tgtgtttcct aacaatggtc ctaaagtaga agcgaccatt accatacctg ggaacatttg
    40561 cacccataat agccatgcaa cgacaaatcc ccatttgctg cccagtgctg tttttaccca
    40621 taactgagga ccgccttctt gaggaagcat cgtggaaagc tcaccggaaa ccattgatat
    40681 cggaccggca aaaaacaaaa ctgcaaaaat tgaataaaaa atcaatgtcc aaccgactgc
    40741 ggctaacgta ggaatactcc gaaccgttgc acacagtgcc attgtcagac cgataaaggt
    40801 cccaagaccc attttactta cttcagattt actcaccgtt gacactctcc ttgatttttt
    40861 ggataaaata acggtgtctt tgaagtagtt tcagcaataa acgaaacaat tcagcgaata
    40921 tttgagggga ttctttgagt ttattgcttg aagggtttct tcaaagacaa cctcaaaaca
    40981 aagggatcga agagcttagg tctccccttg ctcttcagat ccaaacatgc gtcagaaaca
    41041 gttagtattc aagctgtttt ctaacatgat taccttgctt attttgcttc gcttgccaat
    41101 aattggtctg cgtagatttt ttccaatttt tcttgaagag ctgctttgat tttttcagca
    41161 tattcttcaa agtgttcttc tttgttcatg tatggtgtca ttacgctggc acgcaataca
    41221 gttacttttc cggcacggtt ccattcttca tctgagaatc ctagttggtt cacaaattgt
    41281 aatgggctgt tgccatagtc tgggatagca aagtcagtat gtgaagttaa gaactcatta
    41341 ccgtaaatgc ttcctttcac gtatgaagaa taatcatata cgtcatggtt caatttattc
    41401 attgcaacta agtcatcgtt gcctttttct ttgaatacat agtctaccat attgaaatct
    41461 ggataagtta atggatgaac ttcgatttct ttgtctccta ctttaaatga caagtcattc
    41521 aagaagttgt agaaacgatg tgcaccttcg attgatgcac ccattaattt accatatcct
    41581 gtgacattca aaggtaagac atggtgtgct gcccaaacgc tggctgctgt tgcccctgct
    41641 tttgaacctt ccaagatata agccccaagt aatgctggga tatctgcgcc tttttcaaat
    41701 acatatgtcg caaagtaaga gataacgtct ctcatgcgga tatctttgat gacgatacca
    41761 ccggcagaat atggaacata gcccatttta tgtgggtcaa tcgttacaga ttctgcttct
    41821 tcgattgctt tatatgcact gtgtacttct tctaagatat aatctttgtt ttctgtaaag
    41881 acattgtatt tgtaatgaac atcttttaaa tcttcaaatg gaatgaagtt gttgtcttcg
    41941 tctaagaaaa tcgcacggcc gtatccgcca taagcagcat ctacgtgtaa gtagaagtaa
    42001 ataccgtctt tttctaatac gcgacgcaat gcaacgattt tatcgatacc gtcgattgct
    42061 ccttcttctg ttgatcctac aacaccgact acacctagga ttggtgtttt ttcagctgct
    42121 agaccacgaa cgattttttc aagttcgttg atgtccatgc gatagttgtg gtcaactggc
    42181 actggaatca cttggtctaa accgataccg atgatgtcgg cagcttttag ccatgaatag
    42241 tgtttagttt gtggaaccaa ccattttcct aatttttcta aatgttttcc gctacgtgct
    42301 gagtgagctt tgatttcgtc gattttttct gggacgctgt ctaacagatt catgatttct
    42361 tctgttgaca agttcattaa ttcccaatcg ctttttccag caactagttc aggtgtcact
    42421 tctttcattg caagcggcaa tgatttgatg ttgcgggcat accaaagtcc ttctaagttt
    42481 gctaaagaac catcagcaac gatgtggccc cagccatctt tatagctcat taattttgca
    42541 aattccattc ctacttcttc ttccatttgg cttgttgcag gagaagattc ataggcaacg
    42601 ttattaccat tccatagcat agcaaaattg tacgctagta aagatggcat caatgtttca
    42661 gagttcatat gaccccagta acgtccggca ttatgccaag gaacggaatg ggtacgcatc
    42721 cgcgctgaaa tttctgatag aacatccttt gttttattca cagtgtgttc aaaactagca
    42781 ctgctttttt cttctggcgt aataataggc atgtcttgag gcatgtagtt ttgacgccag
    42841 cctaaatgtt catccaccaa ttcattcaac aaagctttat agatttggcc gttctcagct
    42901 ttgtccccaa tgaatagggc atttaggttt agatctttcg acaatgattc actcatagtg
    42961 aaaccctcct aaaaaaatat ttaaattttg tcgagcccta cattacacct gttcttctat
    43021 tctgtaaact aagttttggc ctgattttta atcatatttt aatatgtatc aattatccga
    43081 attgttcaca cttttgaaat aattaaaaaa aacgagggaa tttacaaatg taaactccct
    43141 cgcaaagatt gtattaacga caaccagcgt tctattttag gatgggtttc aaaattggaa
    43201 tgttgtaata gtatttccct ttttcaaggt aaaaacttac taaattctaa tcaattactt
    43261 tggccaaaaa gtaatttttc ttaccttttc gaataaccac aaacttacca tcgaattcat
    43321 cggatggatt tatgacaaaa tttaaatcgg taacgcggtc accgttaata cttatagctc
    43381 cgtttgaaac atcttcacgt gcttgacgtt tagatggttc aatttttgtg gaaacaagca
    43441 gttccacgat attttcaggt gtgcttgaaa tttcaacgtt cggcatttta ccgaatcctt
    43501 gcgcgatttc ttccgcattt agttctttaa tatttccaga gaacaatgct tgcgttattt
    43561 tttgcgcttc tttcaagtct tcttcactat gcacaaatct tgtaacttct tctgccaaac
    43621 ggcgttgtgc ttcacgtttc tcaggttctg tctctacttt tttcgctaag tcttcgattt
    43681 cttcttgaga caagaaagta aagaatttca agtatttcac tacatcgcga tcatcttgat
    43741 tcaaccagaa ttggtagaac tcaaatggtg aagttttctt cggatcaagc caaacggcac
    43801 cgcctgcagt ttttccgaat ttcgttccgt cagccttcag catcaatggg atcgttaaac
    43861 cgaacgcttc tgcttctggt ccttctaatt ttcggattag gtctaatcca gctgtgatat
    43921 ttccccattg gtctgctcca ccgatttgca attgcacatc gtatgtttta tgcagatgca
    43981 aaaagtcgat agattgcagg atttggtaag taaactctgt aaatgagatt cctacttcca
    44041 aacggctggc aacgatatct tttgctaaca tcgtgttgat gttgaagttt ttaccgtagt
    44101 ctctcaaaaa ttcaagtaaa gagatttttg acaaccagtc gtagttgttc acaaatgtga
    44161 tatttgcatc tttaccaaac aattttctca tttgattgga aagtgcgtcc acgttatgtt
    44221 gcacttgttc catcgtttgc aacacacgtt ctgttttgcg gccgcttggg tcgccgatcg
    44281 ttcctgttcc gccgccaatc agaatatatg gatgatggcc tgccaattgg aatcttttca
    44341 tcatcataaa tggaatcaaa tgtccgatat gcatcgaatc accagttgga tcaacgccgc
    44401 aataaagtga aatgctcttt tcttcaacga gctcgcgaag tctttcttcg tttgtttgct
    44461 gattaatggc gtcgcgccaa gttaattcat caataatatt cattttactg acctccgtgt
    44521 agttttttta tgaaagcagg aacctgctag catatgtaat taattatttc ttcaggatag
    44581 gataggaaat aaaaaaagtc ccttggcata taagccaagg gacgaaatct cgcggtacca
    44641 cccaatttgt atgtctagat atccaacata cctctcgaac atttaacgct gtcgcgtggc
    44701 aatcactgcc aaagactcct gaaatgtaat tcgctttaaa gcatgtactg gtttgcagcg
    44761 accaccagct ttctgaaaca ttggctttaa caactacttc gtttcgatct tcgtcttgtt
    44821 attattaata tgtgtctatc atagagcgca actttttctt tgtcaacaga aaaaggatat
    44881 tttttttaaa ttcctcattt tttctttttt gaatccgctc taattccgaa cattttattt
    44941 tcaattattg ttaaaagaaa taactttttc tgtatttttt ctgcaattta tcgagaagtc
    45001 actttacttt gcctcttatt ctttcttcgt tacaataagg aatgggacaa gtaaaaaaat
    45061 gaaaaagcaa gggacgaggt atatggaaaa aaagcaaaaa attattttag gagccacttc
    45121 tgctttaact atcgttattt taattctaag tttgtttttt gtcatccgat catctacaaa
    45181 aaaagaacca atcattcctg aaaaaaaaat aacagaaaaa gcaaaagaac cgacagatga
    45241 taagcaacaa ctagttactc aagcaaaaaa tctggctcaa agctacgatt atgaccaagc
    45301 gattgcgtta ttggaaaaag atagtaagaa cggatctact gaaacacaac agcttcttca
    45361 aacactaaaa aaggagaaag aacaactgat cgactggtct gatcctactc agatctctca
    45421 tgtctttttc cacagcttga tcgtggaccc gcaaaaagcc ttccactctc aacaggcaca
    45481 aggatacaaa gattatatgg tgactgttga agaattcaac cgttcgatcg aacaacttta
    45541 tcaaaatgat tttgttttag ttaatttgaa tgatttgatc caaaaagacg aacaaggaaa
    45601 tctttccttt aacgggcttg cgctacctga aggaaaaaaa ccattggttc tttcacaaga
    45661 tgatgtcagc tattatgaat atatggataa ttccggtttt cctagccgat tggtcgtatc
    45721 aaataacaat gaaatcaaaa atgtctataa agagaaagat aaagaagaaa tcggtgactt
    45781 tgacatggtt cctctgatcg atgcctttgt caagaaacat cctgattttt cttatcgagg
    45841 cgcaaaaggt accttggccc tgacaggtta caacggagtt ttaggttatc gaacatcaaa
    45901 atctcaatat ggagacaatg aaaaaacaaa aaaagagatt aaagaagcta aaaaagtggc
    45961 ggaacagttg aagaaagacg gctggagctt tgcttcccat acatggggtc acttgaatat
    46021 gactcaagct tcactagaag atgtaaaaaa agacaatgaa ttgtggcagc aagaagttgc
    46081 acctatccta ggaaaaacaa atattctgat ttatccattt ggtgcagaca tcagcgactg
    46141 gcagccttat tcagcagaaa atcaaaaatt tgcttacttg aagagtcaag gtttcgatat
    46201 tttctgtaat gtcgatgctt ctacaccagc ttggggacaa ttaggtacgg atttctatcg
    46261 aaatgctcgg atcaatatcg atggcattcg ttttcaatct gatttagacg gaaaaaatcc
    46321 tattcttgat ccattcatca atgtccatga tgtctatgac aaagaagcac gcgaatctgc
    46381 ttgattttac gtcatttatg aaccaaaaaa gccgaactga cattttatcg tcagtttcgg
    46441 cttttttatg ctttatttct tcttcggtgc gattcccttg atcaggactt ctgtccattc
    46501 tgctgtatat ggaagcagtt ttgcgtatat ctccggctta gaaattcctt ctaccattgc
    46561 ttgaaaatag aggatcaaga actcatccga atatttcaaa tccacttttc cttctttccg
    46621 ccctcgatgg aaaagatcaa gcatgatact gaaactttct tgcgtatacg catccatcgc
    46681 gtcagaaaat tctttttttt ctttttttgt atagtatgtc atcaaatcga tataaaattg
    46741 tttgttcact cgattgagat aacggatttt gttttcactc atggcaatca gtgtttcttc
    46801 aaatggaaga ttttccgcaa tcaacgcttt cgcttcttgc cctaaatcat tcataaatgc
    46861 aaaaaatact tcataaatca gcccttcctt gctgccaaaa tatttaaaaa tcgtcgcttt
    46921 acttacctga gcgttctcag ctaccttatc cattgtcact ccagcggttc ctatgtctgt
    46981 gttcatcaag tcaaaagctg cttgtaaaat ctctttcttt ttctcttgtg ctctcttttc
    47041 aaaaccgttc atttgtagag cctcccgtaa ttttaaactt ttataatata tatagttcaa
    47101 atttattctg aggtttatta taactatcta ttgagaaagt atcaagctag caattccttg
    47161 tttatgaatt cattgacaga aatttataaa aagaatatta tgtactaaaa taataaaaaa
    47221 agttttataa aaa
//
LOCUS       DAZKLD010000006        46973 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.006, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000006 DAZKLD010000000
VERSION     DAZKLD010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 46973)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46973)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46973
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.006"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(138..518)
                     /gene="yajC"
                     /locus_tag="ACG8KR_000283"
     CDS             complement(138..518)
                     /gene="yajC"
                     /locus_tag="ACG8KR_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HFQ0199691.1"
                     /translation="MGTLPMLVMFIALLAMWFFMSRSQKKQQQERQNLLDSMKPGDSV
                     VTIGGLHGVVSEIDNDKRTVVLDCEGIFLEYDRAAIKTVKPGTVVTNDATTTVETTEE
                     PAETVEVKTETTETLEETEEKEEK"
     gene            complement(615..1760)
                     /gene="tgt"
                     /locus_tag="ACG8KR_000284"
     CDS             complement(615..1760)
                     /gene="tgt"
                     /locus_tag="ACG8KR_000284"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HFQ0199692.1"
                     /translation="MTQPAIRYRLIKKEKHTGARLGELITPHGTFPTPMFMPVGTLAT
                     VKTMSPEELKEMGAGVILSNTYHLWLRPGEDLVEEAGGLHKFMNWDQPILTDSGGFQV
                     FSLSDMRNIEEEGVHFKNHLNGSRMFLSPEKAINIQNKLGSDIMMSFDECPPFDESYD
                     YVKKSVERTSRWAERGLKAHANPDRQGLFGIIQGAGFEDLRRQSAKDLVGMDFPGYSI
                     GGLSVGEPKSEMNRVLEFTTPLIPENKPRYLMGVGAADSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKYAHDFRPIDEKCDCYTCKNYTRAYIRHLIKCDETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREAFFEEYGFNKENAKSF"
     gene            1978..2676
                     /locus_tag="ACG8KR_000285"
     CDS             1978..2676
                     /locus_tag="ACG8KR_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HFQ0199693.1"
                     /translation="MQSSAELKRQAKESLKGRWGQAVLLNLIPTLIGIATILLIMIPI
                     ILFATTIYDGSASVSEATTSGGGSSGGGGGIISSIISALFMSGISWTYLDILRGQKDQ
                     IEPFKDAFRGFKGLFIGGVISLAVLISIFTTLWGLLLVIPGIIKSYSYSQSYFIYYDI
                     VQETGQTPKVLDTITASRKLMDGYKGQLFWLDLSFIGWHILALATAGIGYLWLSPYIS
                     ATKAAFYEQLPKDI"
     gene            complement(2844..5483)
                     /locus_tag="ACG8KR_000286"
     CDS             complement(2844..5483)
                     /locus_tag="ACG8KR_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFQ0199694.1"
                     /translation="MEEYKQTIEEVKKVTEADPDTGLSSQEVDERRKKQGLNKFDEAP
                     KESMIKKFFRSLSDFTTIILLVAAVISFYTAFATEHGDLFEGLLIIAIVVINSVLAIV
                     QEGNAEKALESLQDMNKQTATVIRDGKVTTVESEQLVVGDILVLESGDAISADARLIE
                     ASQLRVEESALTGESEAVEKDAAFIAEEDESLGDQLNMVFKGCTVAAGRGKAIVTAIG
                     MATEMGKIADLLNENTMQKTPLQVRLNQLGKRISMIALAAAALVFVIGELQGEPLLEM
                     FMTSISLAVAAVPETLTVIVTLTLAYGVQKMARKHAIIRQLPAVETLGTANVICSDKT
                     GTLTQNEMRVRRVWSKEDEVTNIEDSMTNSAMEILKMAALCTDVTVEQEDDDLVIKGN
                     PTEVAIVRAVEENYHTKAELEEKYPRVNELPFDSERKMMTTVHQMGEKYISITKGAFD
                     VLAPRFSSGDVEQAAIVNDRFGKRALRVIAVGYATYDEEPQDISSEALEKDLRLIGLI
                     GMIDPPRPESKGAIKRAKKAGIKTVMITGDHVVTASAIAKELGILNDPSEALSGSELH
                     QLSDEELDARVKALSVYARVTPEDKIRIVKSWQRTGAVVAMTGDGVNDAPALKASNVG
                     CAMGITGTDVAQSAADMILTDDNFATIVDAVSQGRSVYQNIRKAINFLLSCNISEIFI
                     VLIAMLLGWGAPFTAVQLLFVNVVADGLPGFALGREPAEHGIMDQPPIPKNEGIFARG
                     LLQKIAINAGVFTIVTLFGYYLGSYVDTISPWVDASQHVGQTVAFLILAYSSILHVFN
                     VRSSQSIFKVNLATNKALVEMALLALAITTAVALLPFTQELFGLVHISLNHWFLVGIL
                     SIVPIAVNELIKFHRLPEAEEEE"
     gene            complement(6102..7133)
                     /gene="queA"
                     /locus_tag="ACG8KR_000287"
     CDS             complement(6102..7133)
                     /gene="queA"
                     /locus_tag="ACG8KR_000287"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HFQ0199695.1"
                     /translation="MLTTEDFDFDLPEELIAQTPLKDRDHSRLLVLDRKTGEMEDKHF
                     HEIIEELQPGDALVMNNTRVLPARLYGEKPDTGAHLEVLLLTNTQGDTWETLIKPAKR
                     AKVGTRISFGDGRLQAVVKEELEHGGRIIDFEYEGIFLEILESLGEMPLPPYIKERLE
                     DPDRYQTVYAKENGSAAAPTAGLHFTKELLDEIEAKGVELVYLTLHVGLGTFRPVSVD
                     KIEEHHMHSEFYRLTEEAAERLNQVRKNGGKIVAVGTTSIRTLETIGTKFNGEIKADS
                     GWTDIFITPGYEFKVVEAFSTNFHLPKSTLVMLVSAFAGRELTLSAYQHAIEERYRFF
                     SFGDAMFVK"
     gene            7326..8501
                     /locus_tag="ACG8KR_000288"
     CDS             7326..8501
                     /locus_tag="ACG8KR_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Members of the family are the ATP-binding subunit
                     of ABC transporters for substrates such as betaine,
                     L-proline or other amino acids, choline, carnitine, etc.
                     The substrate specificity is best determined from the
                     substrate-binding subunit, rather than this subunit, as it
                     interacts with the permease subunit and not with substrate
                     directly; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="betaine/proline/choline family ABC transporter
                     ATP-binding protein"
                     /protein_id="HFQ0199696.1"
                     /translation="MIEFQNVSKIYKGGKVAVEDVNLSFEKGEFICFIGTSGSGKTTT
                     MRMINRMTDPTQGKILIDGKSIQEIDPVELRRQIGYVIQSIGLMPHMTIRENITLVQK
                     LLKVSQEERNKTAEKMIDLVELPREMLDRYPHELSGGQQQRIGVVRALAANQDIILMD
                     EPFGALDPITRDSLQDLVKDLQERLGKTIVFVTHDMDEAIKLASRIAIMSEGRVIQFD
                     TPENILRHPANDFVEELIGEDRLLQAKPNATRVGEVMLNTAITITPEKSLQEAIKLMR
                     EKRVDTLLVTDNSNVLKGFIDVETLNKKRGKVSSVGDILNKHVFYVKESAYLRDTLQR
                     ILKRGLKYVPVVDEQNKVVGILTRASLVDIVYDVIWGEDPATTDVAESATPEESKEV"
     gene            8505..9146
                     /locus_tag="ACG8KR_000289"
     CDS             8505..9146
                     /locus_tag="ACG8KR_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0199697.1"
                     /translation="MQQFFHQHGSEILSKGFEHLYISGLALIIGIIIAVPLGVLLTRF
                     PKAATIVIGLTSALQTIPSLALLALMIPLFGVGKLPAIIALFIYSLLPILRNTYIGMK
                     NVDWNYRDVAKGMGMTEFQSIFSVELPIAMPTIMAGIRLAAVYVIAWATLASYIGAGG
                     LGDLIFSGLNNYQPDLILAGTIPVTILALLADWLLGLLEHRLTPIALREENEE"
     gene            9143..10060
                     /locus_tag="ACG8KR_000290"
     CDS             9143..10060
                     /locus_tag="ACG8KR_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HFQ0199698.1"
                     /translation="MKRILKMTMVLFSLLILASCSFPGLASNTDEDTISITGGITSEA
                     QILASLVAGMVEHYTDKNTAIINNLATTTINHQAMMNGDASISAARYTGTDLTTTLNL
                     PPEKDPKKAFATVKDEFEKRYGQTWFPSYSFENTYVFLVRKDTAQKYHLSKVSDLKNV
                     ADELVAGVDTSWINRKGDGYDGFQETYGFSFNSILPMQIGLVYDAVEAGKMDIVLGYS
                     TDGRIASYDLVMLEDDRQFFPPYDAAPVVDNKILADTPHLKEALNKLGHTISTEKMQE
                     LNYEADNNLVEPSVVAQRFLQENHYFEGK"
     gene            10057..10728
                     /locus_tag="ACG8KR_000291"
     CDS             10057..10728
                     /locus_tag="ACG8KR_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0199699.1"
                     /translation="MKQLDKMNVFQQFFYYFQENGSYIFAQFIRHFLISIYGVLFAAV
                     VGIPVGIMISRRRKLANWVIRLANIIQTIPSLAMISILIIGLGLGVNVVIVTVFLYSL
                     LPIIKNTYTGMIQVDKNILDVGKGMGMTARQRLFMVELPLSVSVIMAGIRNALVVAIG
                     ITAIGAFVGAGGLGDIIIRGTNATEGTSIILAGALPTALMAIITDWLLGILERRLDPA
                     SRTSR"
     gene            complement(11269..11757)
                     /locus_tag="ACG8KR_000292"
     CDS             complement(11269..11757)
                     /locus_tag="ACG8KR_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosamidase"
                     /protein_id="HFQ0199700.1"
                     /translation="MTRKISKIGASLLVLAVATTIDYRQSEAKTIGNVVYRETAKSML
                     KKAMDNQPESSYWFPEDSLAWSFGKDTDTKYNTSVVPLAERVSKATLPNMNVTQTEEV
                     KVVALSAMNSSINGNASRGIDIFYANVFSYWQYIDQLVYGGALLVKDHRIAKSSCDGC
                     SP"
     gene            complement(11958..12224)
                     /locus_tag="ACG8KR_000293"
     CDS             complement(11958..12224)
                     /locus_tag="ACG8KR_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HFQ0199701.1"
                     /translation="MSNYTVAIKNSAKVDLRKIKQTNLKKQFEEVIQTLKEDPYMPTQ
                     SFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEIYSAWTHYE"
     gene            complement(12224..12487)
                     /locus_tag="ACG8KR_000294"
     CDS             complement(12224..12487)
                     /locus_tag="ACG8KR_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFQ0199702.1"
                     /translation="MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIG
                     LEDWRAIQETLFLEQTGTLDKVRDREKDDSSFTNIDDIDWEAL"
     gene            13008..13148
                     /locus_tag="ACG8KR_000295"
                     /pseudo
     CDS             13008..13148
                     /locus_tag="ACG8KR_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
     gene            complement(13290..15113)
                     /locus_tag="ACG8KR_000296"
     CDS             complement(13290..15113)
                     /locus_tag="ACG8KR_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFQ0199703.1"
                     /translation="MDYLKRLLVGKPLKSAENDEHKLTRFAALALLSSDALSSIAYGT
                     EQIVVVLVALSAAAIWYSLPIAAFVIILLISLTLSYRQIIHAYPHGGGAYVVSSENLG
                     KNAGLISGGSLLIDYMLTVAVSVSAGAEAITSAVPALYGHQVAISVTIVLLLMMLNLR
                     GLRESASFLLFPVYTFILVISLLIVVGLYNIVTGAVPLQATALPGAAVPGVSIALILR
                     AFSSGSSSLTGVEAISNAVPFFKKPRAKNAAATLTMMALILGFFFVGITFINYWYGIV
                     PEKEVTVLSQIGKAVFGHGILYYVLQFATALILAVAANTGFSAFPVLAYNLAKDKFMP
                     HMYQDRGDRLGYSNGIITLALGSIVLLFIFHGSTERLIPLYSIGVFIPFALSQTGMVV
                     KWKKEGKRWLSKSIANITGAFISYAIIAILFVYRLGDIWPFFIIMPIVMFIFYKIHDH
                     YKKVAEQLRLENDAKLHDYDGNTVLVLVGNVTRVNIGALNYARSIGDYVVAMHVSLDE
                     DVEKEKEIEAEFKKHFPDVRFSIVHSSYRSIENPIIRYVDIVSKNAAKQNYTTTVLIP
                     QFVPNRRWQNILHNQTSLRLRLRLSWRENIVVSTYSYHLKK"
     gene            complement(15711..18416)
                     /locus_tag="ACG8KR_000297"
     CDS             complement(15711..18416)
                     /locus_tag="ACG8KR_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip family protein"
                     /protein_id="HFQ0199704.1"
                     /translation="MQSIKNTLRLYLLDWKRIFKNPIATLLIIAIMIIPSLYAWFNIK
                     ALWDPYGNTGELPIAVYSADKPAEFQGKEVAIGEQVIESLHKNKQLGWQFVDSKEQLE
                     DGVRSGKYYAGIYLPKDFSEDLLSFTSGDIKKPKIEYTVNEKINAIAPKITDKGASSI
                     QSQITNEFIKTASSTLLKVFNEIGYDIDTNLVSINKVKDMILSTDENLDTIDGYTKQV
                     LELQSQLPEIKEKLNKANEFVDYIPKVDEMGEKVVALNDKMPELKDQAKIILDLQEKI
                     PEIQNAGKQLAEIDGDFASIQETMNQGIDEAKQGLTIIQQVQDILPDVKNLENQASDL
                     ANQTKSGASQLKDAIPSITNSVDVTLKSIGQVATTTGSLIDTVRTAIEDGKLTDDEKK
                     NISNVLNGFIDGISQQQNAIDKLVEFLTKLQTSAGNTKLQPIIDQLNQLKPLLSNLQN
                     RLSALNTAVQNEDTKAIDELLDQIDAVVNNINGIIAGINTGQISSTVTSLLDQVIDTI
                     GTAQGALSKADQIDFDSLLSSTKATVTNAVAILEKYQKEMPAIGQEIHDANTMLNGNM
                     DTIVNGINEGADLYNNELPIIQQKLGLAADFIQNDWPALKNEITGTLTTVNEKMPEVE
                     TAVNAAADLINNDWPNIKAGIQKAADAIRKGEQEVDLGEVIKLLKLDATKESDFFTQP
                     VELQTNALYPIANNGSASTPFYTALCLWVGAVLLSSVATTEYYLGKKDRNNFTKREQF
                     VARMLTFLTMAIAQSLIVTLGNIFLLGVDVQNPVYSVSFAVLIALAFMMIVYVLAALF
                     DNVGKGIAIIILVLSISGGGGNYPIQVSGKFFQMINPFLPFTHAVNLLRESAGGIYWP
                     NATKAIWIMIGLFVVFGIVGTAIYPFIEEKMTKFKEAAHESHIFH"
     gene            complement(18722..19669)
                     /locus_tag="ACG8KR_000298"
     CDS             complement(18722..19669)
                     /locus_tag="ACG8KR_000298"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HFQ0199705.1"
                     /translation="MRRFAINGYGEAQDVFEEIDAHPREVGSGHVRVEIKAFSVNPYD
                     VALRLGEMKEIRTLKFPYVPGNDGAGIVTEIGSDVTTVHVGDRVAVHAVGGTYGEEVV
                     LPSAKVAKIPDKMSWEEAAGMVTPGITAYNLINHLTEIQPTDTVMILGASGAVGSSLI
                     QLLHEKGIRILTSASSKNEEKVKKLGASAFAAYDKTNPGLQFADQADLVIDATKGSIK
                     GETGIQIMKPGGRYVALNDLPDLDLRQKKEGFYESFVPRKEYLDAEAFAGIIKAYQKG
                     AFHVFISMNLSASLKHVIQAHQLVEGHPPAGKIILSFEK"
     gene            19859..20128
                     /gene="rpsN"
                     /locus_tag="ACG8KR_000299"
     CDS             19859..20128
                     /gene="rpsN"
                     /locus_tag="ACG8KR_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HFQ0199706.1"
                     /translation="MAKKSKIAKAKKQQETIERYAALRQELKAQKDYEGLRKLPLDAR
                     PERLKNRDLIDGRPRGYMRKFGMSRIKFRELAHKGLIPGVKKASW"
     gene            complement(20304..20666)
                     /locus_tag="ACG8KR_000300"
     CDS             complement(20304..20666)
                     /locus_tag="ACG8KR_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFQ0199707.1"
                     /translation="MVKRGDIYFADLSPVVGSEQGGTRPVLVIQNNLGNHFSPTIIVA
                     AITAKMAKPKLPTHIGIKATGTGIERDSVILLEQIRTIDKSRLKEKVCHLDRPIMAEV
                     DRALKISVGIEALTPVTK"
     gene            complement(20677..21798)
                     /gene="alr"
                     /locus_tag="ACG8KR_000301"
     CDS             complement(20677..21798)
                     /gene="alr"
                     /locus_tag="ACG8KR_000301"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HFQ0199708.1"
                     /translation="MVVAWHRPTKAVIHKKAITENVANEVARLPQGKELFAVVKANGY
                     GHGAIETAEAAVAGGASGFCVSNLDEGVELREAGFTQPILILNMVPYDALTVAVAHDL
                     SVTAGTREWLQAAAAVLEKSKLETPLSIHLKADTGMGRIGFCTPEEVKEAAAFIKESR
                     VLEWEGLFTHFSTADQADDTYWNLQKERFIEVLKKLPELPRYVHVSNSATALWHDETI
                     GNMIRYGVAMYGLNPSGHALPEVYPLQPALELVSELIQVKKLPAGEGIGYGETYITPE
                     AEWIGTIPIGYADGWPRKMQGFSLLVEGNYCETIGRVCMDQLMIRLPQEFPVGTKVTL
                     IGKNADKEITMQDIADQLGTIHYEVACGLGQRIPREYQE"
     gene            complement(21814..22164)
                     /gene="acpS"
                     /locus_tag="ACG8KR_000302"
     CDS             complement(21814..22164)
                     /gene="acpS"
                     /locus_tag="ACG8KR_000302"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HFQ0199709.1"
                     /translation="MIKGIGIDAVELPRITRLIEEKPKFIARILTSDEMKLFQSLPFH
                     RQVEFLGGRYACKEAFSKAWGTGIGKVTFQDVEILKNENGQPVVTRSPHEGNVWVSIT
                     HTNETAFAQIILED"
     gene            complement(22294..23805)
                     /locus_tag="ACG8KR_000303"
     CDS             complement(22294..23805)
                     /locus_tag="ACG8KR_000303"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFQ0199710.1"
                     /translation="MKFKELELSPELLKSVERAGFEEATPIQAETIPLALAGKDVIGQ
                     AQTGTGKTAAFGLPMLEKIDPDRHELQGLVIAPTRELAIQTQEELYRLGRDKKVRVQA
                     VYGGADIGRQIRGLKDRPHIVVGTPGRMLDHINRHTLKLGTVQTLVLDEADEMLNMGF
                     LEDIEKIISQVPDQRQTLLFSATMPPAIKNIGVKFMKNPHHVKIKAKEMTADLIDQYY
                     VRAKEYEKFDIMTRLFDVQTPELTIVFGRTKRRVDELARGLEARGYRAEGIHGDLSQQ
                     KRMSVLRSFKSGHLDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KGGMSVTFVTPNEMDYLHVIENLTKKRMTTLRPPTEKEAFKGQLGAAVEQIETKLAEN
                     GLDKYLQTADKLLEEYSAQDLVALLLKTTAKDPADAVPVKITPERPLPMQKKGYNKNG
                     KRGGGNNRNRRDGGNYRGNKSKGGYSKNNNSQKDGGKRHNDKKRGFVIRSNND"
     gene            complement(24127..25479)
                     /gene="murF"
                     /locus_tag="ACG8KR_000304"
     CDS             complement(24127..25479)
                     /gene="murF"
                     /locus_tag="ACG8KR_000304"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HFQ0199711.1"
                     /translation="MNLTIQEIAQAVGSECSALTKITNVEFDSRKITEGSLFVPLAGT
                     RDGHEFIQQAITNGASASFWSLPVEQAPKNFPVIPVADPLKAMQQLATYYLEKVGAEV
                     VAITGSNGKTTTKDLTASVLSEKYKTYKTQGNYNNNIGLPYTILHMPQDTEKIVLEMG
                     MDHANEITELSLMAQPKAAAITMIGEAHIENLGSREGIAKAKMEITDGLVSDGLLVVP
                     ADEPLLTPLTATLVQTVTTFGIGNGDVHAEVTAKGKAQTDFVVEGETFTIPLPGSYNV
                     TNALIAYTIGRFFGLSIPEIRQGLAHVSITQNRTEWLTAGNGAAILSDVYNANPTAMG
                     LVLDTFKNLPTKGRRIAVLADMLELGKESLNMHAQMSEHIDPEKFAMIFLYGEQMHAL
                     KEELNQKYPQLPVFYFEKEKNKLIDAIKQNLEATDSIMLKGSNSMGLFEVVEQLQQMK
                     "
     gene            complement(25632..26708)
                     /locus_tag="ACG8KR_000305"
     CDS             complement(25632..26708)
                     /locus_tag="ACG8KR_000305"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HFQ0199712.1"
                     /translation="MKITLLYGGRSAEHEVSILSAFSVLNAIYYNYYQVQLVFITKEG
                     QWVKGPLLTEKPASKDVLHLSWDPSGQTEEGFTGKVINPGEIKEEGAIVFPVLHGPNG
                     EDGTIQGFLETLNMPYVGAGVLTSACAMDKIMTKYILQAAGVPQVPYVPVLKNQWKEN
                     PKKVFDQCEGSLLYPMFVKPANMGSSVGITKAENREELQNALATAYQYDSRAIVEQGI
                     EAREIEVAVLGNEDVRTTLPGEVVKDVAFYDYEAKYINNKIEMQIPAEVPEEVYQKAQ
                     EYAKLAYTMLGGSGLSRCDFFLTNKNELFLNELNSMPGFTEFSMYPLLWENMGLKYGD
                     LIEELIQLGMNRYHQRQSFFEKNE"
     gene            26904..28649
                     /locus_tag="ACG8KR_000306"
     CDS             26904..28649
                     /locus_tag="ACG8KR_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HFQ0199713.1"
                     /translation="MAQTNPRSRLAEIIRVFINYNVVPNFVQQKNPEQVKKAFEELGP
                     TFIKIGQMLSVREDLLSSAFTQTFKTLQDSVPSDTFSTVKKTIETELSLSLSDIFDDF
                     SKSPFASASMGQAHRAKLKNGDAVVVKIQHPNIAEEIRLDLQLFERAIPLIKYIPETS
                     VVDLKGVLQEVKRSLINEMDFLKESQNGEQFYQKNNGWKEIRSPKIYDAFCSKKVIVM
                     EEMSGKNLNHLMNAENKTETFITGIQNKQLKQEVAKLLVENFMKQVFDDGFFHADPHP
                     GNLLFHVLTKEEQTQASRKTETVHEKEFGSFAFRASTSAEDPVAPYTINYIDFGMMGH
                     LSAGLRQKLTQAVLALYTKDAYRIEKAVLRLCQQEGSFDESRFHQELTSFLEQYYDSP
                     IDEINLQEVFTHVVTICHQNNLQFDRDITLLLKAFGTLEGVIRVLDPEVSLMEVASPF
                     AQHYFLTHLDVEDTLKQSGLDLLEGMKAAPKIPQQLHHLLEMWTSGQGKVNLELKKQD
                     KLLSRIESMINRLVFGMILAALIVGSSLLVQAAPVENAEVVSLLGIFTYAIAAFVIIF
                     LAIDALIQMYKKRKK"
     gene            complement(28929..29366)
                     /locus_tag="ACG8KR_000307"
     CDS             complement(28929..29366)
                     /locus_tag="ACG8KR_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HFQ0199714.1"
                     /translation="MRLFIDGDGSPVKEDVIKLGESYGLDVVIVTSVDHYTTKEYANH
                     VQIVYVDRGADSADYKIVGMVKKNDFLITQDYGLASLVLPKGVRVFHHSGKEFDEANI
                     GTLLEQRYHSGKLRKAGIRTKGPKPFTEKDHMSFRQALKKAID"
     gene            complement(29350..30723)
                     /locus_tag="ACG8KR_000308"
     CDS             complement(29350..30723)
                     /locus_tag="ACG8KR_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0199715.1"
                     /translation="MFEKLKRFGRNFTQTTPFILSNTVIFIPYLLFLGLTDSHNWLTI
                     LPFTLFYAFRMTGLFLVNSLRFGLDSYTLLMIALLIGGAGSLLGILGVFYFPLFYFSS
                     VLLGLSAAWLVPANTTVNFHEKTQGFINMDGKKYLFAAIWLFLLYQAIELPAPTQIAA
                     SFTIYTLFYVMAYHTVSHYPRYELDFKDIKRNLVATRELVLFLIFFGLLFLLRNARLL
                     FDERLFDLAIYGFLLLFILFVFVLGHQKKEWKLPSWLNLFTFLNGMLGNFLFLFSSFY
                     VGILYGLEKLSIFMYLPYAAGLIAAKIVGPWILKRLTWSPLAMQLLGLSSSLLVLLIP
                     NGFAIGVFLLSFWHVVTGSWLNKEYYQTDTAIPMDRRIVVKYTTQNKGSVIHQFLLMT
                     LLFVLAKEMGEPIHLLLITLNHESVPIESVQLLILAKWINVGLLLSGLVTVYHLWKKA
                     KKNEAIH"
     gene            complement(31045..31533)
                     /locus_tag="ACG8KR_000309"
     CDS             complement(31045..31533)
                     /locus_tag="ACG8KR_000309"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0199716.1"
                     /translation="MEKIKVTENELDELMAVIQEVWPEVFVPIIGQKQVDYMLKTYQS
                     KKQIQKELTEGVSYFLLKSEEKTVGYTAYEEINGKIYLSKLYLLNKVRGQGLTSSVFR
                     WYEELAAQTKEREIFLRVNQGNHQAIEVYKHEGFEITEELISDIGQGFQMVDYKMKKA
                     LA"
     gene            complement(31555..32157)
                     /locus_tag="ACG8KR_000310"
     CDS             complement(31555..32157)
                     /locus_tag="ACG8KR_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFQ0199717.1"
                     /translation="MSKKGSVLQKCLQIMPIIGLFFFFVLILYAYHHGIFRSSTSLQA
                     FIQQFGKYAVMIFVLLQVIQVIIPILPGGISSVAGMLMFGNGFGLLYSCIGLVIGEAI
                     GFLLVRYYGVAFVQLILSPKKYQKFDELLTRKTKDIKKVLVVTLLLPFAPDDLICLVA
                     GLTKLSFREFMQIVIFLKPWSVGVYSMIMLFLFHQAQGHL"
     gene            complement(32226..33565)
                     /locus_tag="ACG8KR_000311"
     CDS             complement(join(32226..33124,33124..33565))
                     /locus_tag="ACG8KR_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEnfa3 family transposase"
                     /protein_id="HFQ0199718.1"
                     /translation="MRKYTFVFKKKVVSDYLNNEGGYKYLAHKYQINRTLVRHWVRIY
                     NYHGWEGLVGGGKSYTTKFKLDVIEYMETNGLSIQETAKKFNIGSNRTLSKWIEQYEE
                     GGASSLESQKRGRKISMNSKLNIPKKLKDESLEEEVIRLRAENAYFKKVRNLDSRTRF
                     EQEEIKVKTILQLKKEFKLNLLLSIAQLAKSTYYYWVKKLDKPDKYSKIKQEITAIVK
                     ESRNSYGYRRVTLALKMKGYTINHKTVRKLMSQMGLTCQIRIKRYKSYKGTVGKIAKN
                     VLKRNFSVDTPNKKWVTDVTEFKIKGRKIYLSPILDLFNGEIISYSISTSPTYKLIEE
                     MLQQAIKKKGTEGSLILHSDQGWQYQMPQYQKKLKENNIIQSMSRKGNCLDNSVIENF
                     FGVLKSEFFYREKFRSIEIFQSKLNEYIRWYNNKRIKLKLNGLSPVEYRKQSIK"
     gene            complement(33637..34335)
                     /locus_tag="ACG8KR_000312"
                     /pseudo
     CDS             complement(33637..34335)
                     /locus_tag="ACG8KR_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
     gene            34414..34590
                     /locus_tag="ACG8KR_000313"
                     /pseudo
     CDS             34414..34590
                     /locus_tag="ACG8KR_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348541.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(34766..35335)
                     /locus_tag="ACG8KR_000314"
     CDS             complement(34766..35335)
                     /locus_tag="ACG8KR_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFQ0199719.1"
                     /translation="MELKKLEVPTSSITEVKRSPMDVFAQARDAGTGVYIFNREKVAG
                     VMLTQEQYKTLLQELDVLRQTVKVERKEPKEINEEPINKTASVVSNAEGMEELAQTLQ
                     HSLITGVTITAKNLDERMVALGFITKKTGFGGVVDMLSELMETGKINYQLRRKPQNRT
                     IIAEIIGEQNSQSQLMDKIIIKKIFLHEL"
     gene            complement(35409..36698)
                     /locus_tag="ACG8KR_000315"
     CDS             complement(35409..36698)
                     /locus_tag="ACG8KR_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0199720.1"
                     /translation="MRWQKLLSKNAQNLFSLINLMKDLIWRKADLAEELKIDVKTVDR
                     HLKRLAESDCPIQLILHKKTVQLVYDQGYSEPYLLFVFLIQSPPFCLLKNLLLNEPYE
                     QKYDRSTQERLRKWFGEYHLSFSYKKAQIFGSERKVRYLLFYFLKDFPHFFADDGEQS
                     FSEMFIQLLKQRKDPAEIEILGDSRTLFFELFPELLFREEQILPIKEKNYLAILRLSC
                     NQITEKRWKRIEETQDYRKLTIVTEKLDSLFWLNSEFESESKSKLIKVLYQEWLRYVI
                     GIHDRLIEEVRYYQETLEKFPNSKDKLYQFRLAIQQALAHPAVEWGMLLLKTLQERGY
                     LDVHPPEVKIVFFFRYEHQEARRLAGVLNKTLRHRKRISIHVCTRNKPPRYADLVITD
                     HFFPLDHNEDQKTYLYHPSFGIERLLTDINYWLGKNR"
     gene            37025..37900
                     /locus_tag="ACG8KR_000316"
     CDS             37025..37900
                     /locus_tag="ACG8KR_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010758089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFQ0199721.1"
                     /translation="MGKRSTIQLMIVALLAGGHVLFEDIPGVGKTLLVKALAKAVQGT
                     FSRVQCTPDLLPSDILGFSVYNSQSKEFEFRPGPIFTTILLADEINRTTPRTQSALLE
                     AMAENHATIDNNTYPLDNHFFVLATQNPIEYEGTYPLPEAQLDRFLFRLQIGYPSFDD
                     ELLLLLDKFEKVLDNLNEVLNSYEIEEIKTNVANVFVSPEVASYALQLVSATRSHAAI
                     QLGISPRGSLSFIQAAKAYALIHGRNYVTPKDLQDLVPYVFSHRLIFYDRSLKEDEKK
                     QIIHTLVAQIPIPVR"
     gene            37914..38237
                     /locus_tag="ACG8KR_000317"
     CDS             37914..38237
                     /locus_tag="ACG8KR_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0199722.1"
                     /translation="MEKIKDYFINFVLFLVYFIIVLFALVFNNSLGWFLLAFFTLYVS
                     WSLISLSSFLKKLSVTCTLQPLVYRKKSMQVIFSIAKKRTFSLPLPRLQIGFPAIFAE
                     KNKRF"
     gene            38234..38986
                     /locus_tag="ACG8KR_000318"
     CDS             38234..38986
                     /locus_tag="ACG8KR_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HFQ0199723.1"
                     /translation="MILTNKPQEVQLLWQPKERGCHETLTVIFTAYDSLGLFRKSAKR
                     EISFPVTVLPAFCKIQAEKLQRVLEKKQQLNAYQRGLDFREHRAYRPGDSFNRIDWKL
                     SAHTQEWLYREYDYQEEECSPLFCFWGSPSPKFEPLLDLYFSLWHLRKEKQPELLILG
                     NRAFHGKDPGHTYFASLEAGDEKAFFAHLKKYAKKQIVLFVPENSPEVSEAIETLSKD
                     RTAYLVYFVEKQLMVQEKDKICSLPGGEWIDD"
     gene            38976..41147
                     /locus_tag="ACG8KR_000319"
                     /pseudo
     CDS             38976..41147
                     /locus_tag="ACG8KR_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343611.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
     gene            complement(41259..43430)
                     /gene="copB"
                     /locus_tag="ACG8KR_000320"
     CDS             complement(41259..43430)
                     /gene="copB"
                     /locus_tag="ACG8KR_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper/silver-translocating P-type ATPase CopB"
                     /protein_id="HFQ0199724.1"
                     /translation="MNGNDMEKKHEHKKEVVNKESTESHEKQMEMKHDHSQMDHSMHM
                     GHNHGDIANSKQMDHSMHMDHEHGGMDHSMHMGNFKQKFWLSLILAIPIILFSPMMGM
                     EFPFQVTFPGSDWLVLILATILFIYGGQPFLSGAKMELKQKSPAMMTLIAMGITVAYI
                     YSVYSFIANLLNPHTHVMDFFWELATLIVIMLLGHWIEMNAVSNASNALQKLAELLPE
                     SVKRLTKDGKEETVSLKEVNEGDRLIVRSGDKMPTDGVILKGETIVDESAVTGESRGI
                     KKQANDKVIGGSINGDGTIEIEVTGTGENGYLAKVMEMVRKAQGEKSKLESLSDKVAK
                     WLFYVALIVGILAFIAWLFLTDLPNALERMVTVFIIACPHALGLAIPLVVARSTSIAA
                     KNGLLLKNRNALEQANDLDVIMLDKTGTLTEGKFTVTGVEVLDDAFNKNEILQYLGAL
                     EANANHPLAVGIMNYLKEHEIKPYQAENLKNLSGVGLEATVKKQQVKIVNEKEVERLG
                     LNVEQALLKPYQEQGNTISFLILENHLAAIVALGDVVKTEAKEFIRTLKERKITPVML
                     TGDNKNAAQAVADYLGIEEYYGGLLPDDKEAVVQKYLDQGKKVIMVGDGINDAPSLAR
                     ASIGMAIGAGTDIAIDSADVVLTNSDPKDILHFLDLAKQTRKKMIQNLWWGAGYNILA
                     IPLAAGILAPIGIILSPAVGAVLMSLSTVVVALNALTLKIK"
     gene            complement(43450..45636)
                     /locus_tag="ACG8KR_000321"
     CDS             complement(43450..45636)
                     /locus_tag="ACG8KR_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFQ0199725.1"
                     /translation="MATAETKMDTFVITGMTCANCSARIEKELNGQPGVVNATVNLAT
                     EKASVKYEGTTTEKLIQSVENIGYGAILYDEAHKQKIAEEKQAYLKKMLFDLILSTVL
                     TLPLMLSMIAMMLGSHAAIVHFFHFPIVQLVLSAPVQFYVGARFYKGAYHAIKTKAPN
                     MDVLVAIGTSAAFALSIYNGFFRGHPQDLYFESSSMIITLILLGKYLEHTAKTKTGNA
                     IKQLMSLQTKTAQVIRNGKEETLAIEEVVVGDQLVIRPGEQIPADGRIISGSSAIDES
                     MLTGENLPVEKNPDDTLFGGTINTNGLLHMEVTQVGKQTVLAQIIQMVEDAQGSKAPI
                     QKIADRISGIFVPIVLVIAFITLIATGLITGDWQLALIHSVSVLVIACPCALGLATPT
                     AIMVGTGVGARNGILIKGGEALEAAAHLDSIVLDKTGTITEGKPKVTDLVGSKEVLSI
                     FYTLEQASEHPLGKAIVEYGKLQEAATYDMIDFTAHPGAGISGTINGVRYFAGTRKRL
                     IELNLSFDEYQEHALELEQQGKTVMFLADEKQVIGWIAVADQIKLEAKQAIKQLQNKG
                     LDVFMLTGDNKLAAETIGKQVGIDPKHIFAEVLPEDKAAYVEKLQKDGKKVGMAGDGI
                     NDAPALALADVGMAMGSGTDIAMETADVTLMNSSLASIAQTIELSRLTLRKIKQNLFW
                     AFVYNTIGIPFAALGFLNPIIAGGAMAFSSVSVLLNSLSLNRHMKK"
     gene            complement(45636..45845)
                     /gene="copZ"
                     /locus_tag="ACG8KR_000322"
     CDS             complement(45636..45845)
                     /gene="copZ"
                     /locus_tag="ACG8KR_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HFQ0199726.1"
                     /translation="MKQQFSIEGMSCNHCAARVEETVSALDGVQKVKVNLKKANGTVK
                     FDETKVQSEKICQAINGLGYKAEVI"
     gene            complement(45858..46298)
                     /locus_tag="ACG8KR_000323"
     CDS             complement(45858..46298)
                     /locus_tag="ACG8KR_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HFQ0199727.1"
                     /translation="MEKVKTPVKISDSEWEIMRVIWTLGQTTAQEITQILADSMDWKP
                     ATIKTLLGRLVKKEVIWTEQEGKKFIYHPAVSEMETVRSATENLFSHICAKRIGETIA
                     DLVEEATLTQEDINEILKQLEQKQPVDTIECNCIPGQCECKQHQ"
     gene            complement(46372..46911)
                     /locus_tag="ACG8KR_000324"
     CDS             complement(46372..46911)
                     /locus_tag="ACG8KR_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topology modulation protein"
                     /protein_id="HFQ0199728.1"
                     /translation="MKIIVIGQSGSGKSTLARKIKEITDFPLLPLDLLWHTTDYSIQA
                     KKWFLQEQQSFMVSNPSWIVEGNYTSTLEERIKEADKIIWLKVPRYLAIYRVVWRSLK
                     RKINKKSRPDMPESFSEKLNREYLEFLSFIWHFEENNEPKIQQLIQVANARNKLIILK
                     TRREKQEFLTKLKIESKNN"
BASE COUNT        13341 a         9935 c         8505 g        15192 t
ORIGIN      
        1 ttattattat ttatatgaca cacccgatta ttcaatattg agcaatatat cccatgctct
       61 tatctctttc ttttttgaat aaaaaatgag gtaggacatc tttctgccct acctcgtctt
      121 taaataagct ttgatactta tttttcttcc ttctcttctg tctcttccag cgtttctgtt
      181 gtttcagttt ttacttcaac tgtctcagct ggttcttctg ttgtttcaac agtagtcgtt
      241 gcatcatttg ttacgacagt ccctggtttg accgttttga ttgctgcacg atcgtactca
      301 aggaagatcc cttcgcagtc taaaacaact gtccgtttgt cattgtcgat ttctgaaaca
      361 actccatgta atccgccgat cgttacgact gaatcacctg gtttcatgct atctaacaag
      421 ttttgacgtt cttgctgttg tttcttttgc gaacgtgaca taaaaaacca cattgctaat
      481 agcgcgataa acatcacaag cattggtaat gttcccattt ttttcacctc actaattctg
      541 tatctacaaa aaacacttta gcagaaaaat gctgataaca ctagtttttt acttaatttt
      601 taaaagattt tcctttagaa gctttttgcg ttttctttat tgaacccgta ttcttcaaaa
      661 aaggcttctc taaattcaag caaattatca tccatgattg cttgacgtac ttgtttcatt
      721 agattcaaca agaagtataa attgtggtaa gaagtcaaac gaatcccaaa tgtttcatca
      781 catttgatca aatggcggat ataggcacgt gtatagtttt tacatgtata acaatcacat
      841 ttctcatcaa ttggacggaa atcatgggca tatttcgcat ttttcactac tagacggcct
      901 tgagaagtca tacatgtgcc atttcgagcg attcgagtag gcaagacaca gtcaaacatg
      961 tctactccac ggataacacc atcgatcaat gaatcagcag caccgacacc catcaagtaa
     1021 cgtggtttgt tttcggggat caacggtgta gtgaattcta atacacgatt catttctgat
     1081 ttaggttccc cgacagataa cccgccgatt gagtaaccag ggaaatccat cccgactaga
     1141 tctttcgcgc tttgacgacg aagatcttca aaccctgctc cttggatgat tccaaataat
     1201 ccttgacggt ctggattagc atgagctttc aatcctcgtt ctgcccatcg agacgtacgt
     1261 tctactgatt ttttcacgta gtcgtaactt tcatcgaatg gtggacattc gtcaaaactc
     1321 atcatgatat cagagcccaa tttattttgg atgttgattg ctttttctgg tgataaaaac
     1381 atacgcgaac cgttcaagtg atttttgaaa tgcacgcctt cttcttcgat attccgcata
     1441 tcacttaatg aaaaaacttg aaagccgcct gaatctgtta aaatcggttg atcccagttc
     1501 ataaatttat gcagtccgcc agcttcttct actaagtctt caccagggcg tagccataaa
     1561 tgatatgtat tacttaaaat aacgccagca cccatttctt tcaactcttc tggggacatt
     1621 gtttttactg tagctaatgt tcctactggc atgaacattg gtgtggggaa agttccgtgt
     1681 ggcgtgatca attcccctaa acgcgcgcca gtatgttttt ctttcttgat taaacgatag
     1741 cgaatggcag gttgcgtcat tcatttccct actttctata taaaatatct tgacaactcc
     1801 caccatcata atcgtttcaa gtgctttttg caagttccct cggcttcctt ctactttatt
     1861 taatattctt ttttttatcc tttttttatg aataacaaaa aaaagaaaaa cattatgaca
     1921 gaaatatttt ttgatacatg gtatattggt gttattaaag aaaaggaggg aaaaatcatg
     1981 caatcttctg cagaattaaa acgccaagca aaagagagtc taaaaggacg ttggggacaa
     2041 gctgttttac taaatctaat ccctacattg attggtattg ctactattct tcttattatg
     2101 attcctatta tcttgtttgc caccaccata tatgatggat cagcgagcgt ctcagaagcg
     2161 acaacttcag gaggcggtag ttcaggaggc ggtggcggaa ttatttccag catcattagc
     2221 gctttgttca tgagcggtat ttcttggact tatcttgata ttttacgtgg acaaaaagat
     2281 cagatcgaac cattcaaaga tgcatttcgt ggattcaaag gtttgtttat cggcggagtg
     2341 atcagcttag ctgtgctgat ctcgatcttt acaacattat ggggtttgct attagttatt
     2401 cctggaatca ttaaaagcta ttcttattct caaagctatt ttatttacta cgatatcgtc
     2461 caagaaacag gacaaacacc taaagtactg gatacgatca ctgcaagtcg aaaattgatg
     2521 gatggttata aaggacaact tttctggtta gatctttcat ttatcggctg gcatatctta
     2581 gcattagcaa ctgctggtat tggttatcta tggctcagcc catacatttc tgcaacaaaa
     2641 gcagcatttt atgaacaatt gccaaaagat atttaaaaga gagtgtgact tgtgaccgag
     2701 ggggtggcag gagaacaccg tttatcgaga ggcagtaaaa gaaacattca gcccattagc
     2761 aaaaaggagg tgtgacaaca ttcatgtcac acctcctttt tgtatagttc cagtaaaaag
     2821 aaaatcaact tagaaacagt taatcattcc tcttcttctg cttccggtaa tcgatggaac
     2881 ttgatcagtt cattcaccgc gattggtacg atagataaaa tccctaccaa gaaccagtga
     2941 ttcaaactga tgtgtactag gccgaatagt tcttgcgtaa atggcagcaa agcaacagcc
     3001 gttgtaatag ctaatgccag taacgccatt tctaccaaag ctttattggt cgctaaattg
     3061 actttgaaga tcgattggct gctgcgtaca ttgaacacat gcagaatcga tgaataagct
     3121 aagatcaaga atgcaacagt ttgaccgaca tgctgacttg cgtctaccca tgggctgata
     3181 gtatcgacat aactacctaa ataataacca aacaaggtta cgatggtgaa gacccctgca
     3241 tttatggcaa ttttttgcaa taaacctcga gcaaagatcc cttcattttt cggaatgggt
     3301 ggctgatcca tgatgccgtg ttccgctggc tctcttccta aagcaaatcc tggaagtcca
     3361 tcagcaacta cattgacaaa cagtagttgc acagctgtaa atggtgctcc ccatcctaac
     3421 agcatcgcaa tcaaaacaat aaagatctcc gaaatattac agcttagcaa aaagttgata
     3481 gctttacgaa tattctgata aaccgaacgt ccttgactga cagcgtctac gatcgtcgca
     3541 aagttgtcat ccgtcaaaat catatctgcg gcactttgcg ccacatctgt tcctgtgatc
     3601 cccatggcac acccaacgtt acttgctttc aacgctggtg catcattcac accatcccca
     3661 gtcatcgcta cgactgcacc cgtacgttgc caagatttga cgatccggat cttgtcttct
     3721 ggcgttaccc gagcatagac agagagagct ttgacacgcg catctaattc ttcgtctgat
     3781 aattgatgca gttctgatcc tgaaagtgct tctgacggat cattcaagat acctaattct
     3841 ttcgctatgg cactggcagt caccacatga tctccagtga tcatgactgt tttgatgcca
     3901 gcctttttcg ctcgcttgat tgctccttta ctttctggac gtggtggatc gatcattccg
     3961 atcagaccaa tcaaacgtaa gtctttttcc agtgcttcag aagaaatatc ttgcggttct
     4021 tcgtcataag tagcataacc aacagcaatc actctcagtg cccgtttacc aaagcgatca
     4081 ttaacgatcg ccgcttgttc tacatcgcct gaactaaagc gcggagcaag aacatcgaat
     4141 gctcctttgg tgattgagat gtatttttcg cccatttggt gaactgttgt catcattttt
     4201 cgttcagaat caaaaggaag ctcgtttact cgtggatatt tttcttctaa ttctgccttt
     4261 gtatgataat tctcttcaac agcccttacg atggctactt ctgttggatt acctttgata
     4321 acaaggtcgt catcttcttg ctcgaccgtc acatctgtac atagtgcagc cattttcaat
     4381 atttccatcg cgctgttcgt cattgaatct tcgatattcg ttacttcatc ttctttgctc
     4441 catacccggc ggacgcgcat ttcattttgt gtcagcgttc ctgttttgtc tgagcagata
     4501 acattagcgg ttcctagcgt ttcaactgct ggcaactgtc ggatgatcgc atgtttcctc
     4561 gccatcttct gcacaccgta tgcaagtgtc aacgtaacaa taacagtcaa ggtttccggt
     4621 acagctgcaa cagccaagga aatcgaagtc atgaacatct caaggagtgg ttctccttgc
     4681 agttcaccaa tcacaaatac taaagccgca gccgctaaag cgatcatact gattcgtttg
     4741 cctagctgat tcaatcggac ctgtaaaggt gttttttgca tagtattttc gttcaaaaga
     4801 tccgcgatct tacccatttc tgtagccatg ccaatggcag tgacgatcgc tttcccacga
     4861 cctgcagcaa ccgtacatcc tttaaatacc atattaagtt ggtcacctaa tgactcatct
     4921 tcttcagcaa taaatgctgc atctttttca actgcttcac tttctccagt caacgccgat
     4981 tcttctacac gtaattgtga tgcttcgatc aaacgggcat cggcagaaat agcatcaccg
     5041 gactctaaaa caagaatatc acccactacc agttgctctg actcaacagt ggtcactttg
     5101 ccgtctcgta tgacagttgc cgtttgttta ttcatatctt gcaaggattc cagtgctttt
     5161 tctgcgtttc cttcttggac aatcgccaat accgagttga tcacaacgat agcgatgatc
     5221 agcagtcctt caaataaatc cccatgttct gtagcaaatg cggtataaaa ggaaatcacc
     5281 gctgctacaa gaaggatgat cgttgtaaaa tcggacaagc ttcgaaagaa ttttttgatc
     5341 atcgattctt tcggcgcctc atcgaattta ttcaatccct gcttttttcg ccgttcatcc
     5401 acttcttgtg aagacaaccc agtatctgga tctgcttctg ttaccttctt tacttcttct
     5461 attgtctgct tgtattcttc catcctattc ctcctttaaa atcttcgtac aaaaaaaggt
     5521 acttatttta ctataacatc ctttttttat ttacttcaaa taaagggctt atcgattatt
     5581 tgttaagaaa gcgtaaaatt taaagtgctt atatttgcaa acgctaccaa aggatgctat
     5641 tatatagatg taaggaagga ggataccatc aaatagacag aagaatttct cgatagaaca
     5701 gtgtaagcaa acaaagttgt tagaagtaca ggtttcaccc agacatgcgt gtagagtaca
     5761 aacctataaa tagtgaatgc gtatggatgt ttggatcacg aagtatccga aacaagaagt
     5821 aatgcgatac gtaacaattg aataacgtaa acgtctgaaa attgaatagg aacaagagat
     5881 gtttcaaatg ttgaagatgt aacgaaaaca aaatttttgt ttaacatgaa gaaattcaac
     5941 accgtgcttt gcatgtatga tggaaagtta caatcatttc tgtttatttg aatccttccg
     6001 aggtacaaga aagccgagct tatcgagagc tcggcttttt ctttgtcata aaatctttgt
     6061 aaagcttttt tgttattcaa aactagcggc ttttcataac cttatttcac gaacatcgca
     6121 tctccaaaac taaagaaacg atagcgctct tcaatcgcat gctggtaagc agataaagtc
     6181 aattctcttc ctgcaaaggc actaactaac atgactaaag tcgattttgg caaatggaaa
     6241 ttagttgaga aagcttctac cactttgaat tcatagccag gcgtaatgaa aatatcagtc
     6301 catccgctgt ctgctttgat ttctccatta aatttcgttc caatcgtttc taatgttcga
     6361 attgaagttg ttccgacagc cacgattttc ccgccatttt tacgtacttg gttcaatcgc
     6421 tcagccgctt cttccgtcaa acggtagaat tcactatgca tatggtgttc ttcaatttta
     6481 tctacgctga caggtcggaa tgtacctaac cctacatgta gtgtcagata gactaattca
     6541 acaccctttg cttcgatctc atcaagtaat tctttcgtaa aatgcaatcc agctgttggc
     6601 gctgcagcag aaccattttc tttcgcatag acagtttgat agcgatctgg gtcttccaga
     6661 cgttctttga tatatggagg caatggcatt tctcccaatg attcaagaat ctctagaaaa
     6721 attccttcat actcaaagtc aatgatccgt ccgccgtgtt ccaattcttc tttcactact
     6781 gcttgcaatc tgccatcacc aaaagagatt cttgtcccaa ctttggcacg tttcgcaggt
     6841 ttgatcaatg tctcccacgt atctccttgt gtattcgtta gaagaaggac ttctaaatgc
     6901 gcaccagtat ctggtttttc tccatataaa cgagcaggca gaactcgtgt attattcata
     6961 actagcgcat caccaggctg taactcttca atgatttcat ggaaatgttt atcttccatc
     7021 tctccagttt tccgatccaa aacaagtaaa cgagaatgat cgcggtcttt taatggtgtt
     7081 tgtgcgatta attcttccgg caagtcaaaa tcaaaatctt ctgttgttaa catatcgtca
     7141 ctcactttct atcatgtgct tcatcattct atcatgtttt tcgttctgtt ggcaaaatca
     7201 gcactttttc acttctattt tttgttttcc taaataaaaa aactacccta tttgtatttt
     7261 ttcattgttt taggcatact agtaatagat agttcttcat tgaaaataga aaaagaggtg
     7321 gcaccatgat tgaatttcaa aatgtgtcaa aaatttataa aggtggaaaa gtcgctgtag
     7381 aggacgtcaa tctttcattt gaaaagggtg agttcatttg ttttatcggt acgagcggaa
     7441 gcggaaaaac gacgacgatg cggatgatca accgaatgac tgatccaaca caagggaaaa
     7501 ttttgattga tggaaaatcc atccaagaaa ttgatccagt ggaattgcga cgtcagatcg
     7561 gctatgttat ccaaagtatt ggattgatgc ctcacatgac gatccgtgaa aatatcactc
     7621 ttgtccaaaa attgttgaaa gtcagtcaag aagaacggaa taaaacagct gaaaaaatga
     7681 ttgatcttgt ggaattgccc cgtgagatgt tagatcgcta cccacatgaa ttatctggtg
     7741 gtcagcagca gcgtattgga gtcgttcgtg cccttgcagc gaatcaagat atcatcttga
     7801 tggacgagcc attcggtgcg cttgatccaa tcactcgtga ttccttgcaa gaccttgtca
     7861 aagatttaca agagcgtttg ggaaaaacga tcgtatttgt aacccatgac atggatgaag
     7921 cgattaaact agcaagccgt atcgccatca tgagtgaagg ccgtgtgatc caatttgata
     7981 caccagaaaa tattttacgt catcctgcaa acgatttcgt agaagagttg attggagaag
     8041 atcgcctact acaagccaag ccaaatgcga caagagtcgg cgaagttatg ctaaatacag
     8101 caatcacgat tacaccggaa aaatcattac aagaagcaat caaactgatg agggaaaaac
     8161 gtgtagatac gttgttagtt accgataatt cgaatgtatt aaaaggattt atcgatgtag
     8221 aaacgttgaa taaaaaacga ggaaaagtat ccagtgtcgg cgacattttg aataaacatg
     8281 ttttttacgt aaaagaatcc gcctatcttc gtgatacctt gcaacgcatt ttaaaacgcg
     8341 ggctgaaata cgtgccggtc gttgatgaac agaacaaagt cgtaggtatc ctgactcgtg
     8401 catcattagt ggatatcgtc tacgatgtga tttggggaga agatcccgca actactgatg
     8461 ttgcagagtc agctactcct gaagaatcta aggaggtctg acctatgcag cagttttttc
     8521 atcagcacgg ttcagaaatt ctctcaaaag gttttgaaca tctttatatt tcaggacttg
     8581 ctttgatcat tggtatcatc attgctgtac cgcttggtgt cttactgaca cgttttccaa
     8641 aagctgccac gattgtgatt ggcttaacca gtgccttgca gacaatccct tctcttgctt
     8701 tattggcatt gatgatcccg ctatttggcg taggtaaatt acctgcgatt atcgcactct
     8761 ttatttattc attattgccg attttacgta atacgtacat tgggatgaaa aatgtcgatt
     8821 ggaattatcg ggatgtggca aaaggcatgg ggatgactga gttccaatcc attttttccg
     8881 ttgaattgcc cattgctatg ccaacgatca tggctggtat tcgtttagct gccgtttacg
     8941 tgattgcatg ggctacactt gcctcttata tcggcgctgg tggtttaggc gatttgattt
     9001 ttagtggact gaataattac caaccagatc tcattttggc aggtacgatc cctgtaacaa
     9061 ttttagcact attggctgat tggcttctag gtctcttgga acaccggctg acaccgatcg
     9121 cacttagaga ggagaatgaa gaatgaaacg tatactaaaa atgaccatgg ttcttttctc
     9181 tcttcttata cttgcgagtt gttccttccc tggtcttgcc agcaatacag atgaagacac
     9241 catttcgatc acaggtggaa tcacttcaga agcacagatc ttagcaagtc tagttgccgg
     9301 aatggttgaa cattatacag ataaaaatac cgcaatcatc aacaatctgg ctactacaac
     9361 tatcaaccat caagcgatga tgaacggaga tgcttcaatt tcagcggctc gctacacagg
     9421 aacagattta acgactacat tgaaccttcc tcctgaaaaa gatccgaaaa aagcgtttgc
     9481 taccgtgaaa gatgaatttg agaaaagata cggtcaaacg tggttcccgt cctatagttt
     9541 tgaaaatacg tatgtctttc ttgttcgaaa agacaccgca caaaaatacc atctatctaa
     9601 agtcagcgat ctgaaaaatg tagcggatga attagtagct ggtgtagata cttcttggat
     9661 caatcgtaaa ggagacggtt atgacggctt ccaagaaact tatggcttct ccttcaattc
     9721 gatattacct atgcagattg gacttgttta tgacgcagtc gaagcaggaa aaatggatat
     9781 tgttcttggc tactctacgg atggacggat cgccagttat gatctagtca tgttggaaga
     9841 tgatcgtcaa ttcttcccac catatgatgc tgctccagtt gtagacaaca agatattagc
     9901 tgatacgcct catttaaaag aagccttaaa taagttaggt cataccatca gtacggaaaa
     9961 aatgcaggaa ttgaactacg aagcagataa taatctagtg gaaccatcag tagtagcaca
    10021 acgtttctta caagaaaacc actattttga agggaagtga agcaattgga taaaatgaat
    10081 gtctttcaac aatttttcta ttatttccag gaaaatggct cctatatctt tgcacaattt
    10141 atccgtcact tcctgatttc tatctatggt gtcctctttg cagcagttgt cgggattccg
    10201 gttgggatca tgatttctag gagacgtaaa ttagcaaact gggtgattcg tttggcgaat
    10261 atcatccaga cgatcccttc tctagccatg atctccattt taatcattgg attaggcctg
    10321 ggagtcaatg tcgtgattgt tactgtattt ttgtattccc tcttaccgat catcaagaat
    10381 acgtataccg ggatgatcca agtagacaaa aacatccttg atgtcggcaa aggcatgggg
    10441 atgactgcta gacaacgact attcatggta gagttaccac tctctgtttc tgttatcatg
    10501 gcaggcataa gaaacgcgtt agttgttgcc atcgggataa ccgcgatcgg tgcattcgtc
    10561 ggtgctggtg gtcttggaga tatcattatc cgaggaacca atgcaacaga gggcacatcg
    10621 attattttag caggtgcatt gccaactgca ttaatggcga tcatcacgga ctggctttta
    10681 ggtattttgg aaagacggct agatccagcc agccgtacat cacgctgatg ttattgtaaa
    10741 aaacaaaagc ttctgcgacc atttgttggt cgcaaaggct tttttagttc tgtcattttt
    10801 aaggcaataa tccactctga ttcttccctc cttatgggca aagagttgct tttcttagct
    10861 atttatccat tccgtatact ccaacttcat actgccaacg ggttcatatc tgcatccacc
    10921 gactaagttt ttttcttatc cgctaaggca ttttgccaat ccattttccc atcactcgtc
    10981 atggaattca taccacatga tctctaaata ctcgtctgct tgttctttga attgggcatc
    11041 aattcttaca tcctttggcg tggctttcat ttaagccttt tccatcagtc ctcgattgtt
    11101 cgatgccttc agctgcatca tcaaaactgg tgactgccgt atcggtaact tcgatcatct
    11161 tttatacaat cgggaacttg ccatcctcat ccttggctaa aaacatatct agccattcca
    11221 gcttctcgcc atgggaggtt tgcggttcct aaaaccggta cacgattctt atggactgca
    11281 tccgtcgcaa ctagacttgg cgatacgatg atccttcacc agaagagcac ccccgtaaac
    11341 caattgatcg atatattgcc aataagaaaa gacattggcg taaaagatat cgatccctcg
    11401 tgatgcattg ccgttgatac tgctattcat ggccgaaagc gccacgactt taacttcttc
    11461 cgtttgagtg acattcatgt tcggcaatgt cgctttggat acccgctctg ctaaaggaac
    11521 gacactggta ttgtatttcg tatctgtatc ttttccaaaa gaccatgcca acgaatcctc
    11581 tgggaaccaa taagacgact ccggttgatt gtccattgct tttttcaaca tacttttggc
    11641 tgtttctcga taaacgacat ttccaattgt cttcgcctca ctttggcgat aatcgatggt
    11701 cgttgccact gccaacacta acaacgatgc tccaatttta ctgattttcc ttgtcacact
    11761 tcttcctcct tgttttacat aaacccgtca acattccatt cctaaactca aacaatgacc
    11821 cttcaaaggt ggagatatag gaacaaatta aagaaatcat gcaataaatc caagaaggtt
    11881 ggttttagaa caaaaaaaga gtctcctttt tggggagact ctttgttaac tatctaaaaa
    11941 ggttggctct tctcacttca ctcataatgt gtccaggcag aataaatttc tacaacttta
    12001 ttttcctcat caactttgta aaccactcga tgctgtctat tcagcctgcg tgagtatctg
    12061 ccttcatgtg taggtctcag cttttcaaaa gactgcgtgg gcatatatgg atcttccttc
    12121 aatgtctgaa tcacttcttc aaattgtttc tttaaattcg tttgcttgat ttttcttaaa
    12181 tccactttcg cagagttttt gattgcgact gtatagttac tcatcaaaga gcttcccaat
    12241 caatatcatc gatattcgta aagctactgt cgtctttttc gcggtctcgt actttatcta
    12301 aagtacctgt ttgctcaaga aaaagggttt cttgaatcgc tctccagtct tccagcccaa
    12361 tcaatacagc attgtttcca ctacgatcac taataatctc gatttcagta tgattttcat
    12421 ttacctcttt caataactga taaaaatttt tacgagcagt gctaggattc atcgttgtat
    12481 tagccattgt ctctcctcct ttttctgata taattatagc gtacttgatt aagtacgtca
    12541 aaaaaataca ataaagcact taaattcagc aaacttttcc tcttttttac atagagaaaa
    12601 agaccccttt ttagggagga tctttgtcca cgctcacttc ttactccttt acgactagcg
    12661 gtagccaaac gtaaagtaat tggtaacaac gtatcatctt tagcaaaggt cgaatccaaa
    12721 gcctgtgagt ctatgcaaca acaccaatac acgaaggttc ctcgttgcag tgtgacttga
    12781 ttgatatctt gaataacctg aggatacgca cgaaagattt gtaacggtta ttcaaaactg
    12841 attttggccg tcaagtcttc tactgcctca aaaatgattg cctctttcgt ctcctgtcat
    12901 tcatgttcac tatcgatttg atacttgctt agaccgttgg gtttgtgaat caccaattaa
    12961 aaatggcact gagttatggt aacaaaaaat ggtcacttga cgaggctgag attcctactt
    13021 atgaagacac atggcactta agagtcgagg taaaggaaga agctgaaaat ataggaggct
    13081 gtacattatt tggcaaagga ttcggaaatt atgatcaaga catcaaaatc aaaatgtttt
    13141 acagctaaat aggaagaata ataacatttc atacgctgtt cccatcttta atggcttata
    13201 gagggcacct ttagctgaga ataagacaaa acagaccctc tagcttcaat taagagctag
    13261 agggtctgtt tttgatttta aaacttggtt tatttcttca aatggtagct ataggtgctg
    13321 acaacgatat tttcacgcca agacaatcgc aatcttaacc gcaaacttgt ttggttatgg
    13381 agaatattct gccagcgtct gtttggtaca aattgaggaa tcaacacagt agttgtatag
    13441 ttttgtttcg ctgcattttt acttacgata tctacataac ggataattgg attttcaatc
    13501 gagcgataag aagagtggac gattgaaaaa cggacatctg ggaaatgctt tttaaattct
    13561 gcttcgattt ctttttcttt ttcgacatct tcgtctagcg aaacatgcat cgcaacgaca
    13621 taatcaccga tcgaacgtgc gtaattcaac gcaccgatat ttacccgtgt cacattcccg
    13681 actaatacaa gaacggtatt cccgtcataa tcatgtagtt ttgcatcatt ttctaaacga
    13741 agctgttctg ctactttttt ataatgatca tggatcttat aaaaaatgaa cataacgatt
    13801 ggcataatga tgaagaaagg ccaaatgtcc cctaaccgat agacaaacaa aatcgcaatg
    13861 attgcatatg agatgaacgc acccgtaata ttggcaatcg atttgcttaa ccatcttttt
    13921 ccttcttttt tccatttgac gaccatccct gtttgagata aagcaaatgg aatgaacacg
    13981 ccgatagagt ataacggaat caagcgttct gttgagccat ggaaaataaa cagcaatacg
    14041 atcgaaccta gcgctagtgt gataatccca ttggaatagc ctagtcgatc cccacgatct
    14101 tgatacatgt gaggcatgaa tttatctttt gccagattgt atgcaagaac tggaaaagca
    14161 gaaaaaccgg tgttagcagc aactgctaaa atcagcgctg tagcaaattg aaggacgtaa
    14221 tacaaaatac catgtccaaa gacagcttta ccaatttgag acaaaacagt gacttctttt
    14281 tcaggaacga tcccatacca atagttgata aatgtgatcc ccacgaagaa gaaacctaag
    14341 atcaatgcca tcatcgtcaa tgtagcagcg gcattttttg ctcttggctt cttaaagaat
    14401 ggtacagcat tactaatcgc ctccacccca gttaaagagg aagaaccaga agaaaatgct
    14461 cgtaaaatca aagcgataga aacacccggc acagccgctc ctggaagtgc agtagcttgc
    14521 aatggcacag cacctgtcac gatattatat aaaccaacaa cgatcagtaa cgagatcaca
    14581 aggataaatg tgtacaccgg aaataatagg aacgatgcag attccctcag cccacgaaga
    14641 ttcagcatca tcagtagcaa aacgattgtc acagaaatag cgacttgatg accgtagagt
    14701 gctggcaccg ctgatgtgat cgcttcagca cctgctgaga cagatactgc tactgtcaac
    14761 atgtagtcga tcaataaaga gccaccggaa atcaagccag cattctttcc aagattctca
    14821 ctgctgacga catatgctcc cccgccatga ggataagcat ggatgatttg acgatatgat
    14881 agcgttaatg aaatcaataa aataataaca aaagcggcaa tgggtagcga ataccagatg
    14941 gctgcagcag acaaagcgac taaaacgaca acgatttgtt ccgtaccata tgcaatagat
    15001 gataacgcat cagacgacaa taatgccaaa gcagcaaatc gggttaattt gtgctcatca
    15061 ttttcagcag attttaaagg cttacctact aacaaccttt ttaagtaatc caatttctaa
    15121 actcctttac acacaatttt acttcaagtt ttatagtcga aaatttatag taaacaagat
    15181 gttatagtac gctcactgac cttaaaagtc aataaacttt aagaaaataa ttggaaaaaa
    15241 acagaaaaat atcaagcgct ttttcaaacg ctttaataca gcaaaaatct aacatttttt
    15301 tatgggcttt ttctttgaat tctataataa aaggatagaa cttgacttag aaaatcaagt
    15361 tctatccttt ttgatatctt aacttttgga agagagatgt aaggctgctt tctacccatc
    15421 attcttacta ctcgaagcat agcgcagttg tcacaacctc ttgataaacg gtgtttaaaa
    15481 gcttgcatct gcggtaataa gcccaaccaa attaaaaaca taagaagctg tttttatttg
    15541 gtcgttctta ttacttgatg cagagcagct tttttacaac ctcttggtac acggtgttca
    15601 atcagctgac cctcggtatt agctcaaaaa aacggcaaaa tatgaggaac tatttttcca
    15661 tttttttctc taatactcag gtcaaaacac tgatttcaca acctcttttt ttaatgaaaa
    15721 atatggctct catgcgccgc ttctttgaat ttcgtcattt tttcttcgat gaacggatag
    15781 attgccgttc ctacgattcc aaagacaaca aacaaaccga tcatgatcca gattgctttc
    15841 gtagcatttg gccaataaat tcctcctgca gattcccgta acaagtttac cgcatgagta
    15901 aatggcaaga acggattgat catttggaag aatttccctg agacttggat cggatagttc
    15961 ccgcctccgc cagagatcga cagtactaag atgataatag cgatcccttt accgacatta
    16021 tcgaataatg cagccaagac atagacgatc atcatgaacg ctaaggcgat caagacagca
    16081 aatgacacgc tgtagaccgg attttggaca tctactccta gtaagaaaat attccctaat
    16141 gtaacgatca agctttgcgc aatcgccatt gtcaaaaatg tcagcatacg agcaacgaat
    16201 tgttctcgtt tcgtaaaatt gtttcggtct ttctttccta aatagtattc tgtcgtagca
    16261 acacttgaaa gaagaacagc gccgacccac agacatagtg cagtataaaa cggggtgcta
    16321 gctgaaccgt tatttgcgat tggataaagc gcattggttt gtagctcaac aggttgtgta
    16381 aagaagtcac tttcttttgt tgcgtctagt ttcaataatt tgatgacttc gcctagatcg
    16441 acttcttgtt cgccttttcg gatcgcgtct gctgcttttt ggataccagc tttgatattt
    16501 ggccagtcat tgttgatcaa gtccgcagca gcgttcacag ctgtttcgac ttctggcatt
    16561 ttttcattta ctgttgtcaa tgtaccggtg atttcatttt ttagtgctgg ccaatcattt
    16621 tggataaaat cagcggccaa tcctaatttt tgctgaatga tcggtaattc attgttgtat
    16681 aggtctgccc cttcattgat gccattgacg atggtatcca tatttccatt cagcattgta
    16741 ttggcatcat ggatttcttg tccaattgca ggcatttctt tttgatattt ttctaaaatt
    16801 gccactgcat tcgttacagt tgctttcgtt gagctgagaa gcgaatcaaa gtcgatctgg
    16861 tccgctttgc tcagtgcgcc ttgtgcagtc ccaatcgtat cgatgacttg gtctagtaga
    16921 gaagtgacag tggaactgat ttgacctgta ttgataccag caatgatgcc attgatatta
    16981 ttaacaacgg catcaatttg atctagcaat tcatcaatag ccttcgtgtc ttcattttga
    17041 acagctgtgt tcaaagcaga taggcggttt tgcagattgc ttagcaaggg ttttaattga
    17101 tttagctgat caataattgg ctgtaatttc gtatttccag cacttgtttg caatttagtc
    17161 aggaactcga ccagtttatc gattgcattt tgttgttgag atattccgtc aataaagccg
    17221 ttcaatacat tgctgatgtt tttcttttca tcatcagtca gcttaccatc ttcaatagct
    17281 gttcttacag tatctattaa cgaacctgtc gttgttgcca cttgtccaat tgacttcaat
    17341 gtaacatcta cactgtttgt gatgcttggt atcgcatctt tcagctgaga ggcacctgat
    17401 tttgtttggt ttgctagatc acttgcctga ttttctaaat ttttcacatc tggcaagata
    17461 tcttgtactt gttggatgat cgtcaagcct tgttttgctt catcgattcc ttggttcatt
    17521 gtctcttgga tcgaagcaaa atcgccgtcg atctctgcta gttgcttacc ggcattttgg
    17581 atctctggga ttttttcttg caaatcaagg atgatttttg cttgatcttt caactcaggc
    17641 attttgtcat tcaatgcaac gactttttca cccatctcat ctactttagg gatgtaatcc
    17701 acgaattcat ttgctttatt cagtttttcc ttgatttcag gtagttgact ttgtaattcg
    17761 aggacttgtt ttgtgtagcc gtcgatcgtg tccaaatttt catctgttga taaaatcata
    17821 tctttaactt tattgatact tactaggttc gtatcgatgt cgtaacctat ctcattgaat
    17881 actttcaata aagtcgaact ggctgttttg ataaattcat ttgtgatttg cgattggatc
    17941 gaacttgctc ctttatcggt gattttcggt gcaatcgcat tgattttttc atttaccgta
    18001 tattcgattt ttggtttctt gatatcacca ctagtaaaac tcagcagatc ttctgagaaa
    18061 tcctttggca agtaaatacc tgcgtaatac tttccggaac ggactccgtc ttctaattgt
    18121 tcttttgaat caacgaattg ccatcctaat tgtttatttt tatgaagcga ttcgatgact
    18181 tgctccccaa tggccacttc tttaccttga aactctgctg gcttgtctgc actgtaaaca
    18241 gcaattggca gctctccagt atttccatat ggatcccata atgctttgat gttaaaccac
    18301 gcatatagcg aagggatgat catgatcgcg atgatcagaa gtgttgcaat cgggtttttg
    18361 aaaatccgtt tccagtcaag caaatataat cgaagcgtat ttttgattga ctgcatagtt
    18421 ctcccccttc ttcttttttc agctgaatta ctttaccatg tatttcatac ttttttcaat
    18481 cggctttttt caaaaaagtc gaatttttgg acatatcacg gcaattgtct cattgtaatc
    18541 tcttacatat tgagaaataa ttaaggatta cagcctttac tttatcatga aataaaaggg
    18601 aaagataatt ttatgccaaa gtttttatct attatacgta tacgcaaaaa agctaaaaca
    18661 ttttttgtct ttgcccctac aaaaatgttt cagctttttt cgttttttat tcctgtattt
    18721 cttatttttc gaatgacagg atgattttcc cagcaggagg atgtccttca actagctgat
    18781 gtgcttggat gacatgctta agtgaagcag acagattcat tgaaataaaa acatgaaagg
    18841 ctcctttttg gtaggctttg attatgccag caaaggcttc tgcatctaga tattcctttc
    18901 ttggaacaaa cgattcataa aagccttcct ttttttgccg caaatctaga tctgggaggt
    18961 catttaatgc aacatacctg ccgcctggtt tcatgatctg gatgccggtt tctcctttga
    19021 tacttccttt cgtcgcatcg attacaaggt ctgcctgatc agcaaattgc agacctggat
    19081 ttgttttatc ataagcagca aatgctgaag ctcctaattt ttttaccttt tcctcatttt
    19141 tactggatgc actggttaag attcggattc ccttttcatg gagaagctgg atcagacttg
    19201 acccgaccgc acctgatgct cctagtatca ttacagtatc tgtcggctgg atttccgtca
    19261 aatggttgat tagattataa gccgtaattc ctggcgtgac cattccggct gcttcttccc
    19321 aactcatttt atctggaatt tttgctactt ttgctgatgg caacacgact tcttctccgt
    19381 aagtcccgcc gactgcatgg acagctactc gatcacctac atgaacagtt gtgacatctg
    19441 aaccaatctc tgtcacaata ccagcgccat cattacctgg tacataaggg aatttcaatg
    19501 ttcgtatctc tttcatttct cctaaacgca aggcaacatc ataaggattc acactgaatg
    19561 cttttatttc tacacgaaca tgtccagaac cgacttctct gggatgagca tcgatttctt
    19621 caaaaacatc ttgtgcttct ccgtaaccgt taattgcaaa tcttcgcatg gtcaccatct
    19681 ccctttttct ttaagtttac gtttatgcct ttattttgac aaaaaaaatg tcttttcgtt
    19741 ttggcgcatg attcaaaaca aaatagttgt cttttttcgc gtaacgtgtt aaattattta
    19801 tctaaaagcg taaagattac gatttacgct aataaaaaat aattggagga atttgttgat
    19861 ggctaaaaaa tcaaagattg ccaaagcaaa aaaacagcaa gaaacgattg aacgctatgc
    19921 tgctttacgc caggaattaa aagcgcaaaa agattacgaa ggcttgcgta aacttccttt
    19981 agatgctcgt ccagaacgat tgaaaaatcg tgatttaatc gatggacgcc ctcgcggata
    20041 catgcgtaaa ttcgggatgt cccgtataaa attccgtgaa ttagcacata aaggtttgat
    20101 tccaggagta aaaaaagcca gctggtaatc caagtctggc tgtaatgtaa aggaggaggg
    20161 cgcatggacc actccttgga tcatcaaaaa agatacgttc gcttcttcta gcagaaatgc
    20221 tggtactaga agcagacgta tcttttttta cgcacagctg gtcctttttt atatgtaaag
    20281 gctgcatggt tacttcgcta tatttattta gtaacaggag tcagtgcttc gatacccaca
    20341 ctaattttca atgctcggtc aacttctgcc ataatgggtc gatccaaatg gcagaccttt
    20401 tctttcaatc ttgatttatc gatcgttctg atctgctcca acaggataac ggagtctctt
    20461 tctattcctg ttccagtagc tttgatccca atgtgagtcg gaagcttagg tttagccatt
    20521 tttgccgtta tggccgcaac aatgatcgtc gggctaaaat ggttacctag attattttga
    20581 ataaccaaaa ctgggcgtgt tccgccttgc tccgatccca cgactggaga aagatcagca
    20641 aaataaatat ctcctctttt taccatagga tcctccttat tcttgatatt ctcttggtat
    20701 ccgctgtcct aatccgcaag ccacttcata gtggatcgta cctaattgat ctgctatatc
    20761 ctgcatagtg atttctttgt ctgcattttt gccgataagc gtcacttttg ttccgacagg
    20821 aaattcttga ggcaatcgga tcatcagttg atccatacat acacgcccaa tcgtttcaca
    20881 gtagttccct tctaccaata gagaaaaacc ctgcatcttt cgtggccagc catctgcata
    20941 accgatcggt atcgttccga tccactctgc ttcaggagtg atatatgttt ctccatagcc
    21001 gattccttca cctgctggga gttttttgac ttgaatcaat tcagaaacca gctcaagtgc
    21061 tggctgcaat ggataaactt ccggtagtgc gtgcccagag ggattaagtc cgtacattgc
    21121 tactccataa cgaatcatat tgccgattgt ttcatcatgc cataaagccg ttgcactatt
    21181 gctgacatgt acatatcgag gaagttcggg aagttttttc agcacttcta taaagcgttc
    21241 tttttgcaga ttccaatatg tatcatctgc ctgatctgct gtagaaaaat gggtaaataa
    21301 accttcccat tctaatacac gactttcttt gatgaatgca gctgcctctt ttacttcttc
    21361 aggtgtacaa aatccgatac gacccatccc agtgtctgct tttagatgaa tcgaaagagg
    21421 cgtctctagc ttacttttct ctaaaacagc agctgctgct tgcaaccatt cccgtgtacc
    21481 tgcagttact gataaatcat gagcaacagc tacagtcagt gcgtcgtaag gaaccatatt
    21541 caagataagg atgggctgag taaatccagc ttctctcaac tcgactcctt catctaaatt
    21601 tgaaacacaa aagcccgaag ctccacctgc aactgctgcc tcagctgttt caatagctcc
    21661 atgaccgtat ccgttcgctt ttactactgc aaacaattct tttccttgcg gaagtcttgc
    21721 aacttcattt gctacatttt cagttatagc ttttttatgt atcactgctt tcgttgggcg
    21781 gtgccaagcg acaaccatcg tttttccttc tttctagtct tccaaaataa tttgtgcaaa
    21841 agctgtttca tttgtatgag taatactgac ccatacattg ccttcatgag gtgaacgcgt
    21901 cacaacaggc tgaccatttt cattttttaa aatctcgaca tcttgaaagg taacttttcc
    21961 gatcccagtt ccccaagctt tagagaaagc ctctttgcaa gcgtaacgcc cgcctaaaaa
    22021 ttctacttgg cgatgaaaag gcaaggactg gaatagtttc atttcatcag aggtcaagat
    22081 tcgtgcaatg aattttggtt tttcttcgat cagccgagtg atcctaggaa gttcgactgc
    22141 atcgatccca attcctttta tcatatgctt ttctcttttc aataaattta gaacactctc
    22201 attctaccaa attataacca ttttgccaca aataagtcca caaataacaa aaatgaaagt
    22261 ttctcttaaa aaagagaaac tttcatttta tgtttaatcg ttatttgaac ggattacaaa
    22321 tccgcgtttt ttatcattgt gacgtttacc gccatctttt tggctgttat tatttttgct
    22381 ataaccgcct tttgatttgt ttccacgata attaccgccg tcacgacgat tacggttgtt
    22441 tccgccacca cgtttgccat ttttattgta tccttttttc tgcattggca atgggcgttc
    22501 tggtgtgatc ttcactggca ctgcatcagc tggatctttt gctgtcgttt ttagcaaaag
    22561 tgcaactaaa tcttgtgcag aatattcttc taataattta tctgctgttt gcaggtattt
    22621 atctagacca ttttctgcaa gttttgtttc gatttgttca actgcagcac ctaattgacc
    22681 tttgaatgct tctttttcgg ttggtggacg taaagtcgtc atccgttttt tcgtcaagtt
    22741 ttcaatcaca tgcaagtagt ccatttcatt tggtgtaacg aaagtcacag acataccgcc
    22801 ttttccggca cggcctgtac gtccgatacg gtgaacgtag ctttctggat cttgagggat
    22861 atcatagttg tacacatgag tgacacctga aatatctaat ccacgtgcag caacgtctgt
    22921 cgctacaagg atatctaaat gaccgctctt aaatgaacgc aatacgctca ttcgtttttg
    22981 ttgagacaaa tcgccatgga ttccttctgc acggtaaccg cgtgcttcta atccgcgtgc
    23041 caattcgtct acacggcgtt ttgtacgtcc gaagacgatt gttaattcag gtgtttgaac
    23101 atcaaacaag cgagtcatga tatcgaattt ttcgtattct tttgcgcgga catagtattg
    23161 atcgatcaag tcagctgtca tttctttcgc tttgatcttc acatgatgtg gatttttcat
    23221 gaatttaacg ccaatattct tgatagctgg cggcatcgtt gctgagaaaa gtaatgtttg
    23281 tctttgatca ggaacttgtg aaatgatttt ttcgatatct tccaagaagc ccatattcaa
    23341 catttcatct gcttcatcca aaactaatgt ttgaacggtt ccaagtttca atgtgtggcg
    23401 gttaatgtgg tcaagcatac gtcctggtgt acccacaacg atatgtggac gatctttcaa
    23461 gccacggatc tgacggccaa tatctgctcc gccgtaaacg gcttgtacac gtactttttt
    23521 gtcgcgccct aaacgatata attcttcttg tgtttggata gctaattcac gtgttggtgc
    23581 gataactaaa ccttgtaatt cgtgacgatc tggatcgatt ttttctaaca ttggtaatcc
    23641 aaatgcagct gttttccctg tacctgtctg tgcttgtcca ataacgtctt ttccagctaa
    23701 tgctagtgga atcgtttctg cttggatagg cgttgcttct tcaaaaccag cgcgttcgac
    23761 tgatttcaac aattctggtg ataattctag ttctttaaat ttcaaatgaa tcctcctata
    23821 tggttgttaa ctgaggatgt agcttttttg ctaccccgtc gatttgttcg gtcaaagtgt
    23881 attgtttctt tttgacagtg gccacgcacg tttttatcca ttcttagccc ctgtggcacc
    23941 accgaaaaat cactttgcga agtttaggca ttagaaaagc caaacgggtc gcgttcagct
    24001 ttatcattat tatgacatct ggcttcaaca agttgtttct atggcaaaac cagtccaatt
    24061 tctcttcatc tttcggcaat aggttattct aacatagttt ttcgttttct gcaagtttac
    24121 agatttttat ttcatttgct gaagttgttc taccacttca aataagccca tgctgttgct
    24181 gccttttagc ataatactat ctgttgcttc aagattttgt ttgatagcat caatcaattt
    24241 attcttttct ttttcaaaat agaacacagg tagttggggg tatttttggt tcaattcttc
    24301 ttttagtgca tgcatttgtt ccccataaag gaaaatcatg gcgaatttct caggatcgat
    24361 atgttcactc atttgcgcat gcatatttaa agactctttc cctaattcca gcatatctgc
    24421 taacacagcg attctgcgtc cttttgttgg cagattttta aaagtatcta ataccagtcc
    24481 catggctgtt ggatttgcat tgtagacatc gctcaaaata gctgcaccat ttcctgccgt
    24541 tagccattcg gttctgtttt gagtgataga aacatgggcg agcccttggc gtatctcagg
    24601 tatggacagt ccaaagaagc gcccgatcgt atacgcaatc aaagcattcg ttacattata
    24661 acttccaggc agcgggatcg taaatgtttc tccttctact acaaaatcgg tttgtgcttt
    24721 tccttttgct gtcacttccg catgaacatc gccatttcca ataccgaatg ttgtgactgt
    24781 ttgtactaat gttgccgtta aaggtgtcag caacggttca tctgcgggca caacgagtaa
    24841 tccgtctgat acaagtccgt cagtgatttc cattttggct ttagcgattc cttctcgcga
    24901 cccaagattt tctatgtgtg cttcaccgat cattgtgatt gctgcagctt ttggttgagc
    24961 cataagtgaa agttccgtga tttcattggc gtgatccatg cccatttcca aaacgatctt
    25021 ttctgtatct tggggcatgt gcaaaatcgt gtaaggaaga ccaatattat tgttgtaatt
    25081 tccttgcgtc ttatatgttt tgtatttttc agacaagaca gacgcggtta gatctttcgt
    25141 ggtggtttta ccattgcttc ctgtaattgc aacgacttct gcgcctactt tttccaagta
    25201 ataggttgcc aattgctgca tggctttcaa aggatctgct acaggaataa ctggaaaatt
    25261 ttttggagct tgttcaacag gcaagctcca aaatgaagca gatgctccgt ttgtgatcgc
    25321 ttgctggata aattcatgtc catctcgcgt accagcaaga ggaacgaata aacttccttc
    25381 ggttatcttc cggctgtcaa attcgacatt ggtaatcttt gtcagtgcag agcattcgct
    25441 gcccacggct tgtgcaattt cttgtattgt tagattcatt gagatctctc ctaaaaaagg
    25501 ttgtgaaaaa agcgtttggc ctaaacggcg ggttcgcatg actctcactt ccaagtaaat
    25561 ttctctattc gtatttagaa gctagaatga acgcggttac gcaatcactc cagcctcttc
    25621 tttatttctc tttattcatt tttttcaaaa aaagattgac gctgatggta tcgattcatt
    25681 cctaactgga tcagttcttc aatcaaatca ccgtatttca agcccatatt ttcccataag
    25741 agtgggtaca tactgaactc cgtaaatcct ggcatagagt ttaattcatt caggaataat
    25801 tcatttttat ttgtcaaaaa gaaatcgcac cggctcaatc cgcttccacc taacatcgtg
    25861 taagctaact tcgcgtactc ttgcgctttt tgataaactt cttctggcac ttcggctgga
    25921 atctgcattt cgattttatt attgatatat tttgcttcat aatcatagaa tgctacgtct
    25981 tttacgactt caccaggcaa agtcgtccga acgtcttcat ttcctaatac agcaacttcg
    26041 atttcgcgcg cttcaattcc ttgttcaacg attgctcgag aatcatactg ataggctgtt
    26101 gctaaagcat tttgcagctc ttctcggttt tctgcttttg taatgccgac actagaaccc
    26161 atattcgccg gtttgacaaa catcggataa agcaaagaac cttcacattg atcaaatact
    26221 tttttaggat tttctttcca ttgattctta agtactggta cataaggaac ttgcggcaca
    26281 ccagcagctt gtaaaatata cttggtcatg attttatcca tggcacatgc actggtcaat
    26341 acgcctgcgc cgacataagg catattcaat gtctctaaga agccttggat cgttccatct
    26401 tccccgtttg gcccatgtaa aactggaaaa acgatggctc cttcttcttt gatttcgccc
    26461 ggattgatca cttttcctgt aaagccttcc tctgtctgtc cacttgggtc ccatgaaaga
    26521 tgcaagacat ctttgctggc aggtttttct gttagtaatg gacctttgac ccattgtcct
    26581 tcttttgtaa taaatacgag ttgaacttgg taataattat aataaatggc atttaaaact
    26641 gaaaatgcgg aaagaatgga cacttcatgc tctgcgctgc gtccgccata tagtaaagta
    26701 atcttcaaaa gattgtcctc ctgtattcta aatcgttcag tgatttactc tcatatttta
    26761 gcacaaaaca aaaataaacg aataacttat ttataaaaaa acgagccagc ttctaaaaga
    26821 acgagcgtca tttatttttt gtcagctaga ttatttgtcc cgttttttat aaaaagttat
    26881 gataaagaaa aggagggatg atgatggcgc aaacaaatcc acgcagccgc ttggcagaaa
    26941 tcattcgagt gttcatcaat tacaatgtag tgccaaactt tgtacagcaa aaaaatcccg
    27001 aacaggtaaa aaaagccttt gaagaactag gccccacctt catcaagata ggacagatgc
    27061 tttctgttcg agaagatttg ctttcttctg ccttcactca aacattcaaa acattacaag
    27121 atagtgtacc tagcgacaca ttttcaacgg tcaaaaagac gatcgaaaca gaactttctc
    27181 tttcattatc agatatattc gatgattttt caaaatcgcc ttttgcctct gcttctatgg
    27241 gccaggctca tcgtgccaag ctgaagaacg gtgacgcggt agtcgttaag atccaacacc
    27301 ctaacatcgc cgaagagatc cgacttgatc ttcaactatt tgaacgagcg atccctttga
    27361 tcaaatacat tcctgaaact agcgtagttg atctaaaagg ggtactccaa gaagtaaaac
    27421 gttccttgat caatgaaatg gacttcttaa aagaaagtca aaatggcgaa caattctatc
    27481 aaaagaacaa tggctggaaa gagatccgca gccccaagat ttatgacgct ttttgttcga
    27541 aaaaagtgat cgtgatggaa gaaatgtctg gtaaaaactt gaatcattta atgaacgccg
    27601 aaaacaaaac tgaaactttt atcactggaa ttcaaaacaa gcagctgaaa caagaagttg
    27661 caaagctttt agttgagaac tttatgaaac aggtgtttga tgatggtttt ttccatgctg
    27721 atcctcatcc agggaatctg ctattccatg tactaacaaa agaagaacag acacaagcta
    27781 gccgaaaaac tgaaacagtc catgaaaaag agttcggttc ctttgctttt cgagcctcca
    27841 cttctgcaga agaccctgtt gcgccatata ccatcaacta catcgatttt ggtatgatgg
    27901 ggcatctttc tgcaggactt aggcaaaaac tcacacaagc cgttttagcg ctgtatacga
    27961 aagatgctta tcgaatcgaa aaagcggtcc ttcgactttg tcagcaagaa ggctcgtttg
    28021 atgaaagtcg gttccaccaa gaactgacaa gttttcttga acagtattat gattctccta
    28081 ttgatgaaat caacttgcaa gaagtattca cacatgtcgt gacgatttgt catcaaaata
    28141 atctgcagtt cgatcgtgat atcacgcttt tgctgaaagc atttgggaca ttagaagggg
    28201 tgatccgcgt cctcgatcct gaagtttccc tcatggaagt cgcttcgcct tttgctcaac
    28261 attattttct cactcatctg gatgtagaag atacattgaa acaaagcgga ttagacttat
    28321 tggaagggat gaaggctgcg cctaagatcc ctcaacagct ccaccatcta ttagaaatgt
    28381 ggacttcggg acaaggaaaa gtcaatttgg aattgaaaaa gcaagacaaa ttattgagca
    28441 ggatcgaaag tatgatcaat cggcttgttt tcggtatgat tttagctgca ttgatcgtag
    28501 ggtcctccct gcttgtccaa gcagcacctg tagaaaacgc agaggtcgtt tccttattag
    28561 ggatatttac gtatgctatt gccgcattcg tcattatttt tttggcaatc gatgcattga
    28621 tccaaatgta taagaagagg aaaaagtaga aaaaggttgt gacagaaaga atcagcttca
    28681 agaaataaga aggggtttta cataaattgt ttctcaaatt tttgtgaaaa ttcggcttat
    28741 ttttcgaaga ggctacttct gaaacaccga gaataaaaaa tgtgaaaaag ctgttctgca
    28801 cccagtaata agaacaatca aaaagggaac atggaacaca tttatcctgt ttccctgttc
    28861 cctttttgat gtattcgagc ataaataaaa taccttcgtt acctactatc ttctgtactt
    28921 ggtaaacctt agtcaattgc cttttttaac gcttgacgaa aagacatatg atctttctcc
    28981 gtaaagggtt ttggaccctt tgtccttatg ccagcttttc ttaactttcc actatgataa
    29041 cgttgttcta gaagcgtgcc gatattcgct tcgtcaaatt cttttccaga gtgatggaac
    29101 actcgcacac ccttcggcaa tacaagtgaa gctaaaccat aatcttgcgt gatcaaaaag
    29161 tcattcttct taaccatacc gacgatttta taatccgcac tatcagctcc gcggtctacg
    29221 tagacgattt ggacatgatt agcgtattct ttggtcgtat aatggtccac actggtcacg
    29281 ataaccacgt caagaccata ggattctcct aattttatga catcttcttt tactggagaa
    29341 ccatctccat caatgaatag cctcattctt tttcgccttc ttccataaat gataaacagt
    29401 tactaatccg ctcagtaaaa gaccaacatt gatccatttt gccaagataa gcagttgtac
    29461 actttctatc ggcactgatt catggttcag cgtgatcaat aatagatgga ttggctctcc
    29521 catttctttt gctaaaacaa acaaaagcgt catcaataaa aactggtgga tcacactccc
    29581 tttattttga gtagtatact tgacaacgat ccggcgatcc attggaatag ctgtatcagt
    29641 ttgataatat tccttattta gccagctgcc tgttactacg tgccagaaac taagcagaaa
    29701 gacacctatc gcaaaaccat ttggaatcaa gaggaccaat aaagaagaac tcagtccaag
    29761 caattgcata gccaatggag accatgttaa acgtttcaaa atccatggtc cgactatttt
    29821 tgctgcaatc aagcctgctg cataaggcaa atacataaaa atcgaaagtt tttctaatcc
    29881 gtacagtatg ccaacataaa aactgctaaa taaaaaaagg aaattcccta gcattccatt
    29941 tagaaaagta aacagattta gccagcttgg caacttccat tccttcttct ggtgtcctaa
    30001 aacaaacacg aacaagatga acagcagcaa gaaaccataa atagcaagat caaaaagcct
    30061 ttcatcgaat aacaatcggg catttctcaa aagaaacaat aagccaaaga atatcaaaaa
    30121 taaaacaagc tctcgcgtag ctaccagatt tcttttgatg tctttgaaat ccagttcgta
    30181 acgcggataa tgactaaccg tatggtaggc catgacgtaa aaaagtgtat aaatagtaaa
    30241 gcttgctgca atctgagtcg gtgcaggcaa ctcaatcgct tgatacagga gaaatagcca
    30301 gatagctgca aataagtact ttttcccatc catattgata aaaccttgcg tcttttcgtg
    30361 aaaattgacc gtggtattag caggtacaag ccatgctgca ctcagcccta gcaaaacact
    30421 tgaaaaataa aataaaggaa aataaaatac gcctaatatt ccaagcaatg aaccagcacc
    30481 accaatcagc agagcgatca tcagcaacgt gtaactatct agaccaaatc gtagactgtt
    30541 gactaaaaat agtccagtca tccgaaaggc ataaaacagc gtaaatggca aaatcgtcag
    30601 ccaattatgc gagtctgtta gtcccaaaaa taaaagataa ggaataaaaa taaccgtgtt
    30661 acttaagata aagggcgttg tttgtgtaaa attacgccca aatcgtttga gtttttcgaa
    30721 catgacaatg gctccttaaa cagtcttata actaatctgc tcttcgattt tagcattaaa
    30781 ggggaaataa accaatttta cagaatatat tcttgtaaaa aaaaatacag aaagaagcat
    30841 aagcttcttt ctgcattttt acggtacttg attactcaag cagacaagcc cttctccaca
    30901 aaagtcttgc ccgacacccc tgcatccggc aggtgataaa gatagaaatc aattatcctg
    30961 tgcctccaaa atcaattagg agctctgtaa accagagtcc ttctcgaccc gtcgcataaa
    31021 tttactttaa cacagttcct atgtttatgc gagagctttt ttcattttat aatcgaccat
    31081 ttgaaatcct tgaccgatat ctgaaataag ttcttctgta atctcaaatc cttcatgctt
    31141 ataaacctcg attgcttgat gattcccctg attcactcgc aaaaagatct ctctttcctt
    31201 tgtttgcgca gcaagttctt cataccagcg aaaaactgaa gaagtcaatc cttgaccacg
    31261 tactttattt agcaaatata atttacttaa ataaattttt ccatttatct cttcataagc
    31321 agtgtaaccg acagtctttt cttcgctttt taataaaaag taggacacac cttctgtcag
    31381 ttctttttga atctgttttt ttgactgata agttttcagc atgtaatcca cttgtttttg
    31441 accaatgatc ggcacaaaaa cttccggcca tacttcttgt atgactgcca tcagctcatc
    31501 cagttcattt tctgttactt ttattttttc catacaccca cgtccttttt tacattataa
    31561 atgaccttgc gcttgatgga ataaaaagag catgatcatg ctatacacac ccaccgacca
    31621 cggtttcaag aaaatcacaa tctgcataaa ttccctaaaa gataatttcg tcaaaccagc
    31681 tactagacag atcaagtcgt ctggcgcaaa gggaagcagc aaggtaacaa ctagtacttt
    31741 tttaatatct ttcgtttttc ttgtcagcaa ttcatcaaat ttctgatact tttttggcga
    31801 taaaataagc tggacaaagg ctacgccata gtaacggaca agcaaaaatc cgattgcttc
    31861 cccaatcact aggccaatac aactatataa aagcccaaat ccattgccaa acatcagcat
    31921 gcccgctact gaggagatcc ctcccggcaa aataggaatg atcacttgga tcacttgcaa
    31981 cagaacaaat atcatcactg catacttgcc aaattgctgg ataaacgctt gcaaagacgt
    32041 gctcgatcga aaaatcccat gatgataagc ataaagaatc aacacaaaga aaaaaaacag
    32101 accaataatg ggcatgatct gaagacattt ttgtaaaact gatccttttt tagacatcta
    32161 tattccccac atcttttttc atttattaaa actgcctcct tatgaactga accccaaaag
    32221 ttgaactatt taatggactg tttccgatat tctactggag atagtccatt taactttagt
    32281 tttatccttt tgttattata ccacctaata tactcattta atttactttg aaatatctca
    32341 attgatcgga atttttctcg ataaaaaaac tctgatttaa gcacaccaaa aaaattttct
    32401 attacagaat tatctaagca atttcctttt ctagacatgc tttgaataat gttattctct
    32461 tttaattttt tttgatattg tggcatctga tactgccatc cttgatctga atgtagaatc
    32521 agtgaaccct cagttccctt ttttttaatt gcttgttgaa gcatttcttc aatcagttta
    32581 tatgttggac tagttgatat actataacta attatttctc cgttaaataa atcaagtata
    32641 ggagatagat agatttttct tccttttatc ttgaattcag tgacgtctgt cacccatttt
    32701 ttattgggtg tatctactga aaaatttcgt tttaacacat tcttggcaat ttttcctact
    32761 gttcctttat aagatttata tcgctttatt cggatttgac aggtaagtcc catttgggac
    32821 attaattttc taactgtttt atgattgatt gtatatcctt tcatctttaa cgctaaagtg
    32881 actctacgat aaccataaga gtttcttgat tcttttacaa tcgctgtaat ttcttgcttt
    32941 atcttgctat atttatctgg cttatctaat ttttttaccc agtaataata agttgacttc
    33001 gctaattgtg caattgaaag taatagattc aatttaaatt cttttttgag ctgaaggatt
    33061 gttttaacct tgatttcttc ttgctcaaat cttgttcttg aatcaaggtt tctaactttt
    33121 ttaaatatgc gttctctgct cttaaacgaa taacttcttc ttcaagagac tcatctttaa
    33181 gttttttagg aatgtttagc ttggaattca tactaatttt tctgcccctt ttttggctct
    33241 caagtgaaga agcaccgcct tcttcatatt gctctatcca tttacttaga gttctatttg
    33301 aaccgatatt aaatttttta gcagtttctt ggatagaaag accatttgtt tccatatatt
    33361 ctataacatc aagtttaaat tttgtagtgt agcttttgcc acctccaacc aagccttccc
    33421 aaccatgata gttataaatc cttacccaat gtctaaccag tgtacgattt atttgatatt
    33481 tatgtgcaag atatttgtag ccgccttcgt tatttaaata gtctgaaact acttttttct
    33541 taaaaacaaa tgtatatttc cgcaaaaaaa gcaccccttt taattagatt tctagtctaa
    33601 cttttggggt gcacatcaca aagagggggg gattttttat cgttgtggaa tatttaaata
    33661 ccaacgcttg tcatgaaaat cttgtgctcc acccttagta tttccttctg gatcgtttgt
    33721 cgcacgcatc atgacgtata ctttcttatt agggaaatca cgcatattga aagatacatg
    33781 atagccaaca tttcctaaag tattataagc ttgatttacg tctggtctat aaatcccatc
    33841 agctcttact ctagctagtt cttttccagt attgtagtcc ataatgaaaa tatactcgta
    33901 tttataatta gcaatgtgcc atccagctac atgcaagttt gcgttttcga tttctccgaa
    33961 ctgatcaatg tgggcgtaat ttgttccatc tgtcagcgta gaatttgcag cacctgctcg
    34021 agttggatca attactggtt tatcctccga agtagttgga ttatcatccg taaaaccatg
    34081 agccaaatca taggctagtt tttctttact tacacccatt tcagaaagat aaccgtaagg
    34141 atctgtatga tcgccccaaa tattttgtgt tacccataaa tgcgatttga ttcctggttg
    34201 gttataagga gtgtctaatg ttaatggaat accatatttc attgctgaat ctcttgccaa
    34261 ttcaacgtat actttatagt tcttttcaaa agtttcttta tcatgtgtgt gttgtaactc
    34321 gatttgcaca ggacttttgg ctcacgaggt tgtcctactc tacatcttgc tgatagatat
    34381 gctgaaagga aaacaccaac attatgtttt gctttttcag ctagaacgag acaagcagga
    34441 atacctttag aagatatcaa agatttttta ggtcataaag atgtttccac tacacaagtc
    34501 tatgcccata tttctcccga agtgaaaaaa agatcaatga atcaactaga aaactatata
    34561 gaagagcaaa taaaaaagca ctcaaattga gtgctttttt aagctcacta tcactaaaac
    34621 tatcactaat tttatttata agcgaacatg tttctatcac tgatttttta aatctagcga
    34681 aaaaccctta tcttaaaggc gttcgttcaa ttctttggca aggtcttcaa agcctggctt
    34741 gcctagaagt gcgaacatgg ttttcttata gctcatgtag aaaaattttc ttgatgatga
    34801 ttttatccat caattgagat tgactgtttt gctctcctat gatttcagct atgattgtcc
    34861 gattttgcgg ttttcttctt aactggtagt tgattttccc agtttccatc aactcgctaa
    34921 gcatatccac gactccgcca aatccggtct ttttcgtaat aaaacctagt gctaccattc
    34981 gttcatctag attttttgct gtaatcgtca caccagtaat gagcgagtgc tgtaatgttt
    35041 gggccagctc ttccatccct tcagcattgg atacgacgga agctgttttg ttaataggtt
    35101 cttcgtttat ttcttttggt tctttcctct ccactttcac tgtttgacga aggacatcta
    35161 gttcttgcaa gagcgttttg tattgttctt gggtcagcat cacgccagct actttttcac
    35221 gattaaatat atatacgcca gttcccgcgt ctcgcgcttg agcaaataca tccatcggcg
    35281 atcgtttcac ttctgtgatc gaagatgtgg gaacttctaa tttctttaat tccaatggat
    35341 ttcctccttt actaatcgct ttttattata gcacagactg acttgttttg atcttttttc
    35401 caaaggtttt atcggttttt gcctaaccag taattgatat cggtaagaag tctttcgata
    35461 ccaaatgaag gatggtacag atacgttttt tgatcttcgt tatggtctag tggaaaaaaa
    35521 tgatcggtga tcactagatc tgcataccga ggcggcttat ttcgagtaca tacatgtata
    35581 gagatccgtt ttcgatgtct tagcgttttg ttcagtaccc cagcaagtct tcttgcttct
    35641 tggtgttcat agcgaaaaaa aaagacgatt ttcacttcag gagggtggac atctaaatac
    35701 cctcgttcct ggagtgtttt cagtaaaagc atcccccact ctactgcggg gtgtgcaagt
    35761 gcttgttgga tcgctagccg aaattgatat agtttatctt tggaattggg aaatttttct
    35821 aatgtttcct gataataacg aacttcttct atcagccggt catggatacc aattacgtaa
    35881 cgaagccatt cttggtaaag tacttttatc agctttgatt tgctttcgct ctcaaattcg
    35941 ctattcagcc aaaacagcga atcaagcttt tcagtcacaa tcgtcaattt ccgatagtct
    36001 tgtgtctctt ctatccgttt ccaccgcttt tcagtaatct ggttacagct caagcgcaaa
    36061 attgctagat aatttttttc ttttattggc aaaatctgct cctctctaaa tagcaattca
    36121 ggaaacaatt caaagaatag tgttcgactg tctcctaaaa tctcgatttc tgccggatcc
    36181 tttctttgct tcaataattg gatgaacatc tcagaaaagg actgctctcc atcatctgca
    36241 aaaaaatgcg gaaaatcttt caaaaagtaa aacagcagat atcttacttt tctttctgaa
    36301 ccaaagatct gtgctttttt ataagaaaaa gaaaggtggt actcgccaaa ccactttctc
    36361 aatcgctctt gcgtagaacg atcgtacttc tgctcgtaag gttcatttaa tagcagattt
    36421 ttcagtaaac aaaacggggg tgattggatc agaaaaacaa acaagagata aggttcgcta
    36481 tatccttgat cataaactaa ctgaacggtt ttcttatgaa gaatcagctg aatgggacag
    36541 tcgctttccg ccaaacgttt cagatgccga tccactgttt taacatcgat tttcagttct
    36601 tcagctaaat cagcttttcg ccagatcaaa tctttcatta gattgattag tgaaaataaa
    36661 ttctgtgcat tttttgataa tagtttttgc catctcatcg atcatttctc acctgatttc
    36721 taaacatatt gtttgatcaa aatgagttca ctcctaaaaa gataaaaaac tccctttcta
    36781 tttatttaaa tacgcaaatc ttcctcatta ttttttattt tactcaattt aatcgtattc
    36841 tcatttcaaa acgaatgtac tttaaaaaaa cataataaga tcactttgct tgtaggatgt
    36901 attcccttcc attacaatga aataaatagc gattttatga gaaggtgatg tgaaaatgga
    36961 agaaaaaata gaagaatcca aacaaaaaat cgatcagttg atataagaat tagaaaaagc
    37021 ggtcatcggc aaacgttcaa ctatccaatt gatgatcgtg gccctattag caggtggtca
    37081 tgtcttattc gaagatattc ccggtgtagg taaaacgtta ctggtcaaag cactagcaaa
    37141 agctgtacag ggaaccttca gcagagtcca atgtactcct gatctgctgc ctagtgatat
    37201 tcttggtttt tccgtataca attcacaatc aaaagagttt gaatttcgtc cgggaccgat
    37261 ctttaccacg atcttgttgg cagatgagat caatcgtacg actcctcgta ctcagtcagc
    37321 acttttagaa gcaatggccg agaatcatgc aacgatcgac aacaatacat accctctaga
    37381 caatcatttt tttgtcttag ctacgcaaaa tccaatcgag tacgaaggaa catatccttt
    37441 gccggaagca cagctcgatc gttttttatt ccgactacaa atcggttatc cttcttttga
    37501 tgacgaactt ttgctacttt tggataaatt cgaaaaagtg ttggataact taaatgaagt
    37561 attgaacagt tatgaaatag aagaaataaa aacaaatgtg gcgaatgtct ttgtcagtcc
    37621 cgaagtggca tcttatgcct tgcagttagt gtcagcaaca agaagtcatg ctgccatcca
    37681 gcttggcatc agtccacgag gaagcctttc ctttattcag gcagcaaaag cttatgcttt
    37741 aatccatggg agaaattacg tcacgcccaa agatttgcag gatttagttc cttacgtgtt
    37801 ttcccatcgc ctgatttttt acgatcgttc attgaaggaa gacgaaaaga aacaaatcat
    37861 tcatactctt gttgcacaaa ttcctattcc tgtgaggtag attgaggtag acaatggaaa
    37921 aaatcaaaga ttatttcatt aattttgtgt tatttctggt atatttcatc attgtgcttt
    37981 ttgctcttgt attcaacaat tctttaggct ggttcttgtt ggcctttttt actctttatg
    38041 tatcatggag cttgatttca ttaagcagct ttctaaagaa actttcggtt acttgtacat
    38101 tgcagccact cgtttatcgc aaaaaaagta tgcaggttat tttttccata gcaaaaaaga
    38161 gaactttttc tttacctctc cctcgtttac aaatcggctt cccagcaatc tttgcagaaa
    38221 aaaacaagag attttgattc ttacaaataa accgcaagaa gtccaattgc tttggcagcc
    38281 aaaagaaaga ggatgccatg aaacattaac tgtcattttt acagcctatg atagtctagg
    38341 acttttcaga aaaagtgcaa aaagagagat ctcttttcct gtgactgttc tgcctgcttt
    38401 ttgtaagatt caagcagaaa aattacagag agttctggaa aaaaaacagc agctgaacgc
    38461 gtaccaaaga ggtcttgatt ttcgggaaca tcgcgcttat cgtccaggtg attctttcaa
    38521 ccgaatcgat tggaaattgt ctgctcatac tcaagagtgg ttataccgtg aatacgatta
    38581 tcaagaagaa gaatgttctc ctcttttttg cttttggggt tcgcccagtc caaaatttga
    38641 acctctgcta gacttgtatt tttctttatg gcatttgaga aaagaaaagc agccggaact
    38701 tctgatatta ggcaaccgcg cctttcatgg caaagacccc ggtcatacct attttgcttc
    38761 tttggaagct ggcgatgaga aagctttttt cgcacacttg aaaaaatatg caaaaaaaca
    38821 aatcgtcctc tttgttcctg agaattctcc tgaagtctca gaagctattg aaacattgtc
    38881 aaaagatcga acggcttatc ttgtttattt cgtagaaaaa caactcatgg tccaagaaaa
    38941 agacaagata tgttcattgc caggaggtga atggattgac gattaaacgg gaaaaatggc
    39001 tgcgtggcgt ttactccttt gtgatactaa gtgtaggtgc tccgatattt gtagtagcta
    39061 atcaattgca aaacagctac cttcttatcc taactattgg tgtcctatgc cttattgtct
    39121 gctgtattcc taaaaaatgg gtacgtttgc tgctttcttt gccagtaatg ataggttgtt
    39181 tgtttttata ttttccttcg aagacatttt ccatattatg gatcttacag tttttttcgt
    39241 cagttacaga ggaattcttc catctatccc aaggtaaact ttattatttg ccagaaaaga
    39301 cagctttttt tctcataatg ttgctgatct atttatttat tacattgacc gtggattacg
    39361 actattggta cgcacccttt ctttctttga tggcttactt catgatgctt accgtttttc
    39421 ggaaaaaaga tttactgtac gaaatgattg cggtagtaac tgttttgttc gtgtatctgg
    39481 cagcaaggca atttttctcc atccggttgt tgtcgggaaa cactcgattc cagtcattga
    39541 ctacagttct gcttatttgt ttcttaactt ttagcgctat cttgccttta tatcttccga
    39601 aagtacacca atctctcgag gaaacgagtg aaccgatccg agaatatctt aatgatgaag
    39661 ggctttatga cacactccat gagtatcaat taaccggaag tgcaagaaca ggcttcagtg
    39721 agaatgatga acagcttggt ggacctttat atgataatgg cgaagttgtt tttacagcga
    39781 accaaaaagg aaatggctat tggaagatcg aaaataaaaa tcattacacc ggttctggat
    39841 ggatagaaga tcaggacgca cccatccaac caatccaaga attccctgtt gacatcacga
    39901 gctaccaaac cgttatagaa gacaaagaaa acattacatt gaccctaaaa aaacaagctt
    39961 tcttacctta tccttatgga aatactacct tgactgattt ttcattagct agtggacaaa
    40021 cgctttacta tttgcctgaa agccagcgat tcattcctgt cccgaatgaa tcgacacgtt
    40081 ctgcaacttt acaaacagaa aggttagttt ttacgagaga agaattaaaa agcagtcagt
    40141 taaaagctga tgatttagaa aatcagcaga caaatcttca attgccaaaa agtttgccta
    40201 aacgtattga ggacttggcg gtcgaattga ccaaaaatga agcaacttta attcgtaaag
    40261 tcgaagctgt ctaacagtat ttaaaaacaa gcggaaattt ccgctattcc aaaacggata
    40321 ccgggcatac tccgaaagaa cacgattatg tggattattt cctctttgat tcaaaagtcg
    40381 gctattgtga caacttttca acttctatgg tcgtccttct tcgaacactt ggtatcccag
    40441 ctcgctggac aaaaggtttt acaaatgggc tcagaacagg cacagataat caagggaatg
    40501 aggaatacag cattctcaat agtcatgctc attcttggcc agaggtatac tttcctggat
    40561 ttggctggct tccatttgaa ccaacccctt catttgccaa tccaatgggt tcacaaccag
    40621 ttgctgaaac aaccgaaacc gtccagcctg ctatcgaaca accaaatgaa agcgaaaata
    40681 tggcagaaac tatttccagt gagagatctg aggtcattcc atcagaagaa acaacactca
    40741 caccagctta taaaatcgtc ttattcagcc ttggagtgtg tttatccgtc ttgttgttct
    40801 ttctattgtt attcagaaaa tctctgcgct ggacaagtta tcttatctat cttcattttt
    40861 ttgcttctac taatacgatt gcatatcaaa aacttcttaa ccctttggaa acattggtac
    40921 cgcgcaaacc cgcagaatct ttgcaggaat atagcctgcg aatagaacaa gaattcaagc
    40981 tagatctgcg tttcattcga ttaactactc aatatgaagc agaaatatat ggacgacaag
    41041 cagatccggt aaaattcgat aaaaaaacct atttagaaat aacgcgtata ataagaaaaa
    41101 cggtatacaa aaaaattctg acaaaaggga gccgcttcaa aaaataagat aaaatttctg
    41161 gagaatctac ttctggaatg ccgagaacca gctgattgag tatcgattac tcgaaaaaaa
    41221 ggttgtgaca cgttttttgt cacaaccttt tttcattatt atttgatttt caatgtcaaa
    41281 gcatttagag ctacaactac cgtacttaac gacatcaaga cagcaccaac tgctggactt
    41341 aatatgatac caattggcgc aagaattccg gctgctagcg gaattgcaag aatattgtaa
    41401 cctgcccccc accagaggtt ctggatcatt ttctttcgcg tttgcttggc tagatcaaga
    41461 aaatgcaaaa tatcttttgg atcgctgttt gtcaatacga catccgcaga atcgattgcg
    41521 atatctgtcc ctgcaccgat tgccatcccg attgaagcac gggctaaact tggcgcatca
    41581 ttgatcccat cacccaccat gattactttt ttcccttggt caaggtattt ctggacaaca
    41641 gcttctttat catctggtaa taaaccgcca taatattctt cgatccctaa ataatctgct
    41701 actgcttggg cagcgttttt attgtcacca gtaagcatga caggagtgat ttttctttct
    41761 ttcaatgttc ggataaattc ttttgcttct gtttttacta cgtcacctaa agcaacgatt
    41821 gcagctagat gattttctaa aatcaggaag ctgatggtat ttccttgttc ttggtaaggt
    41881 ttcaataatg cttgttctac gtttaatccg agacgctcaa cttctttttc attaactatt
    41941 ttcacttgtt gttttttgac tgttgcctct agcccgactc ctgaaagatt ttttagattt
    42001 tcagcttgat aaggcttgat ttcatgttct ttcaaataat tcataatgcc aacagccaat
    42061 ggatgattgg cattcgcttc caaggctcct agatattgaa ggatttcatt tttattgaac
    42121 gcatcatcaa gtacttcgac acccgttaca gtaaattttc cttctgttag tgttcctgtt
    42181 ttgtccagca tgatcacatc tagatcattg gcttgctcca gtgcattgcg gtttttcaaa
    42241 agtaatccgt ttttagctgc tattgatgtg gagcgagcaa caactaatgg gatagctaaa
    42301 cctagagcat gaggacaggc gatgataaag actgttacca tacgttctaa tgcattagga
    42361 agatctgtca agaacaacca ggcgatgaat gccaggatac ccacaattaa tgcgacataa
    42421 aacagccatt ttgccacttt gtcagataat gactctagct tggatttttc tccttgtgct
    42481 tttcgcacca tttccataac cttcgctaga taaccatttt caccagttcc agttacttcg
    42541 atttcgatcg ttccatcccc attgatagaa ccgccaatca ctttgtcatt ggcttgtttt
    42601 ttgattcctc ttgattcgcc agtcacagct gattcgtcaa caattgtttc acctttcaag
    42661 attacaccat ctgttggcat tttatcacca gaccggacga tcaaacgatc accttcgttt
    42721 acttctttca atgaaactgt ctcttctttt ccatctttcg tcaagcgttt cactgattct
    42781 ggcagtaatt ctgctagttt ttgtaaagca ttgctggcat tcgacacagc attcatttca
    42841 atccagtgac ctagtagcat aataacgatc aaagtagcca attcccagaa gaaatccatg
    42901 acgtgtgtat gtggattgag cagatttgcg ataaacgaat agacactata aatatatgca
    42961 actgtaatcc ccatcgcgat cagtgtcatc atagcaggac ttttttgttt caattccatc
    43021 ttagctccac ttaaaaatgg ctgaccgcca tagataaata gtatagttgc taagatcaat
    43081 acgagccaat cagaaccagg aaacgttact tgaaatggaa attccattcc catcattggt
    43141 gaaaaaagaa tgattgggat agctaggata agcgatagcc aaaatttttg tttgaaattc
    43201 cccatgtgca tggaatgatc catgccgccg tgttcgtgat ccatgtgcat cgaatggtcc
    43261 atttgtttgc tgtttgctat atctccatgg ttatgtccca tgtgcatgga atgatccatc
    43321 tgactatggt catgtttcat ctccatctgt ttttcatgag attctgtact ttctttgttt
    43381 actacttctt ttttatgctc atgttttttc tccatatcgt tcccattcat ttttactcca
    43441 tcccttctct tactttttca tgtgtcgatt taagctcagg gagttcaaca atacactgac
    43501 ggaactaaac gccatcgctc ctcctgcaat gattggattc aaaaagccta gtgcggcaaa
    43561 aggaatacca atcgtattat agacaaaagc ccagaaaaga ttttgtttga tttttctcaa
    43621 tgtcaaccta gacaactcga ttgtttgtgc aatggatgcc aaactgctat tcatcagcgt
    43681 cacatcggct gtttccatcg caatgtccgt tccacttccc attgccatac cgacatcagc
    43741 taatgctaag gccggtgcat cattgattcc atcgcctgcc ataccgactt ttttgccatc
    43801 tttctgaagt ttttctacgt aggctgcttt atcctctgga agtacttcag caaagatatg
    43861 ctttggatca ataccaactt gtttaccaat cgtttcggct gccagtttgt tgtctcctgt
    43921 cagcataaaa acatctaatc ctttattctg gagctgcttg attgcttgtt tggcttctag
    43981 tttgatttga tcagccacgg caatccaccc gatcacttgc ttttcatcag ctaaaaacat
    44041 gactgttttt ccctgctgtt ctaattctaa cgcgtgttct tggtattcat caaatgatag
    44101 gttcaattca attaatcgtt tacgtgtacc cgcaaaatag cgtactccat taatcgtacc
    44161 gctgatccca gcacctgggt gggctgtaaa atcaatcata tcgtatgttg ctgcttcttg
    44221 cagttttccg tattccacga ttgctttacc tagtggatgt tcagaagctt gttctaaagt
    44281 ataaaaaata gataggactt cttttgaacc gactagatct gtaactttgg gctttccttc
    44341 tgtaatcgtt ccagttttat ccaaaacaat gctgtctaag tgagcagctg cttctaatgc
    44401 ttctccgcct ttaattaaaa ttccatttcg tgcaccaaca cccgttccta ccataatagc
    44461 tgttggggtc gctaaaccta atgcacatgg acacgcaatg actaatacag agacactatg
    44521 gatcaatgcg agttgccaat caccagtgat caaacctgta gctatgagcg tgataaacgc
    44581 gatgactaat acgatcggaa caaaaatccc tgagatccga tctgcgattt tttggattgg
    44641 ggctttgctc ccttgagcat cttccaccat ttgaatgatt tgcgctaata ctgtctgctt
    44701 gccgacttgt gtgacttcca tgtgtagcag accattcgta ttgattgtcc cgccaaaaag
    44761 cgtgtcatcc ggattctttt ctacaggaag attttcacca gtaagcatac tttcatctat
    44821 cgcactgctt cctgaaataa ttcgaccatc tgctggaatc tgctcaccag gacggatgac
    44881 tagttgatca ccaacgacta cttcttcaat cgctaacgtt tcttctttcc cgtttcttat
    44941 cacttgggcg gtttttgttt gaagagacat caattgtttg attgcattac ctgttttcgt
    45001 ttttgctgta tgttccaaat atttgccgag caaaatcagc gtgatgatca tactgctgct
    45061 ttcaaaataa agatcttggg ggtgtccacg gaaaaatccg ttgtaaatgc tcaatgcaaa
    45121 ggcagccgat gttccaattg ctaccaatac atccatattt ggtgctttcg tcttgattgc
    45181 atggtacgca cctttataaa aacgagcacc gacataaaat tggacaggag cagacagcac
    45241 taactggacg attggaaaat gaaaaaagtg aacaatcgcc gcatggcttc ctaacatcat
    45301 agcaatcatt gacaacatca acggcaaagt caaaaccgta ctcaatataa gatcgaatag
    45361 catttttttc aaatacgctt gcttttcttc tgcaattttt tgtttgtggg cttcatcgta
    45421 caaaattgca ccataaccga tgttttctac gctttggatt aatttttcag tggtcgtccc
    45481 ttcatatttg acacttgctt tttcagtggc caagttgact gtggcattga ccacaccagg
    45541 ttgtccgttc agctcttttt cgatcctagc agaacaattt gcacaagtca tacctgtaat
    45601 gacgaacgta tccattttgg tttctgctgt tgccatcaaa tcacctctgc tttataaccc
    45661 aatccattga ttgcttgaca aatcttttcg gattgaactt ttgtttcatc aaacttgacc
    45721 gtaccgtttg cttttttcaa atttactttc actttttgca cgccgtcaag tgcgcttact
    45781 gtttcttcta ctcttgctgc acaatgattg cagctcatac cttcgattga aaattgttgt
    45841 ttcatttttt cttcctccta ttgatgttgt ttacattcac attgacctgg gatacagtta
    45901 cattcgatcg tatctactgg ttgcttttgt tccagttgtt tcaaaatctc attgatatct
    45961 tcttgagtca aagtggcttc ttctactaga tccgcaatcg tctctccaat cctttttgca
    46021 cagatatggg aaaaaagatt ttccgtcgca ctgcgaactg tttccatctc cgagacagct
    46081 ggatgataaa tgaatttttt tccttcttgt tctgtccaga tcacttcttt ttttaccaaa
    46141 cgtcccagca aagttttgat cgttgctggt ttccaatcca tcgaatcagc taagatctgc
    46201 gtgatttctt gggctgtcgt ctgtccaagt gtccaaataa cacgcatgat ctcccactct
    46261 gaatcactga tctttactgg cgttttgact ttttccattt atttcacacc tttcgtttac
    46321 aaatgtaatc attagcttat gaatacagat tacgcttgta aacaaaaact gtcaattatt
    46381 tttgctctct attttcaatt tagttaaaaa ttcttgtttc tctcgtcttg ttttaaggat
    46441 gatcagttta tttcttgcat tagctacttg gatcagctgc tgtattttcg gttcattgtt
    46501 ttcttcaaaa tgccagataa aagataaaaa ttctaagtat tccctgttta gtttctctga
    46561 aaatgattca ggcatatctg gccgagattt tttgtttatc tttcttttta gagaacgcca
    46621 aactactcgg tagatggcga gataacgcgg cactttaagc caaatgatct tatctgcttc
    46681 tttaatcctt tcctctaatg tagaagtata atttccttca acaatccatg acggattaga
    46741 taccataaac gattgctgtt cttgcaaaaa ccactttttt gcctgaatac tgtagtctgt
    46801 tgtatgccat aaaaggtcta gcggcagtag cggaaagtcg gtgatttctt tgatcttcct
    46861 tgcgagggtc gattttcccg acccactttg tccaatcacg atgatcttca ttcttctccc
    46921 tcacttctct ttggaatttg gttcgatcat accacttata acgccgcaaa aaa
//
LOCUS       DAZKLD010000007        42461 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.007, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000007 DAZKLD010000000
VERSION     DAZKLD010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 42461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42461
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.007"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            175..1167
                     /locus_tag="ACG8KR_000325"
     CDS             175..1167
                     /locus_tag="ACG8KR_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0199729.1"
                     /translation="MVTIKDVAKAAGVSHTTVSRALNNSSLIKNETKQKIRQLAKEMN
                     YSPNYSAKGLVNQKKYLIGLFFSSLHKGTSSNFLADSIAGIHSVLDTNYSLSVESIDV
                     VDLTEINLQRYDGLIVMSQSDDDQPFIDYLKEKEFPFVVVNRHINDPAVINVTADDAA
                     GVMQAVDYGIGCGHTKIAYIGGKKNFRSTKEREKGVLKSLKKASIPVNPAYFLTGDYS
                     LESGLQNMEYLLSLSDLPTLVFCGNDDIAIGALRTVHLAGLRVPQNISLIGFDDSPVV
                     SYLNPPLTTVRKPLKEMCQEGTKLLLDLIEGRTIEQSKIELSTLLILRESINYL"
     gene            1227..2648
                     /locus_tag="ACG8KR_000326"
     CDS             1227..2648
                     /locus_tag="ACG8KR_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HFQ0199730.1"
                     /translation="MKKLTKELVKKNEAPVKVLQFGEGNFMRGFIDWQIQQLNNQQLF
                     KGNVAIIQPLEQGLGNMMNQQDNLYTVILQGLSNGEIVDTSEIITVVEQMINPYDEWD
                     AYLSLAENDDLAYIVSNTTEAGIQFVETDNLTDTPPQSLPGKLTAFLYRRFKLNKAGF
                     TIIPCELIDRNGERLKEIILRYAEKWDLEPAFVEWLEKENVFCCSLVDRIVPGYPRED
                     AGVFNQRHNYEDQLMVKAEPFMLWVIEGPKELENQLPFKQAGLNVIITDDMTPYRERK
                     VHLLNGPHTAMVPLALLAELETVEEVMKDPLFSAYVDQLFNLELIPMLSLPKDELAIY
                     ADQIKERFLNPFAHHKLEAISLNSVSKFSTRLLPVFKKYIEEQNQVPPLITVSLAALL
                     LMYRGDQVKPHDDEKTISEFTDAWSDNGTAIPRLLQNAALWGEDLSQIPNVTDTVQEI
                     AAKIDQKGVRSVLQEMKGTMVHA"
     gene            2641..4140
                     /locus_tag="ACG8KR_000327"
     CDS             2641..4140
                     /locus_tag="ACG8KR_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HFQ0199731.1"
                     /translation="MLETLQPTMKLNRRDSVVVALTPIPKQTQLTIDQQTITTLEDIP
                     QGHKIALGDLKAGDNVIKYGYPIGHVTTDVTAGQWLHTHNVKTNLSGELDYRYEKDVH
                     PSHYLFENRTFQGYLRKNGKVGIRNDLFLVPTVGCVNGIAELIVKQFKEKHPDLGQFD
                     HITILKHPYGCSQLGKDHQNTREILADAVNHPNAGGVLVFGLGCENNTVPEFKKILGE
                     YDEERVKFLVAQDVYDEIEQGVALLEELLTAAENDQRISVPLSKLNVGLKCGGSDGLS
                     GITANPLLGAFSDYLIAQGGSTILTEVPEMFGAEQVLMARAENEEVFDSIVDLINDFK
                     QYFLSYGEPVYENPSPGNKAGGITTLEDKSLGCTQKSGSSPVVDVLQYGEKIRKPGLS
                     LLQAPGNDLVAASALASSDCQLVLFTTGRGTPFGSYVPTVKVSTNTTLFERKGHWMDF
                     NAGVLLEQPMEVILESFVQKIIAVASGEETKNEQNDVREIAIFKNGVTL"
     gene            4179..5573
                     /gene="uxaC"
                     /locus_tag="ACG8KR_000328"
     CDS             4179..5573
                     /gene="uxaC"
                     /locus_tag="ACG8KR_000328"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HFQ0199732.1"
                     /translation="MFLSDDFLLKDDWAKKLYHSYAKNMPIIDYHCHLDPKEIYENNN
                     FSNLTEAWLGGDHYKWRLMRACGVPEEKITGNASDFEKFLAWCQTVPKIIGNPLYSWS
                     HLELKRFFGIDLLINEENAEKIWTQANEILAAPDFRRREIAKKSNVEVICTTDDPADD
                     LHYHQLLAHEEPDLKVLPSFRPDKALNIEKEGFNAWVTLLEEAADQTITTYQELIEVL
                     GQRIEYFHQHGCRVSDHALDILRYKEADETVLEEIFQKARANKLLSSDEIDAYRTETL
                     IHLTHFYHSHNWTMQLHIHAYRNCNTQMLEKLGPDTGYDGMNDLSLTIPLQKLLNRAE
                     ETDQLPKTILYSLNPNDYPAILALMGCFQKEQNGKLQLGSGWWYNDTRAGMRDQLTQF
                     ADGSALGNFVGMLTDSRSFLSYTRHEYFRRVLCEFLGEMVMRGEAPEDEQLLGSLVQA
                     ISYTNAKNYFGFFA"
     gene            5732..6415
                     /locus_tag="ACG8KR_000329"
     CDS             5732..6415
                     /locus_tag="ACG8KR_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HFQ0199733.1"
                     /translation="MPTIHIAGDSTAAEKDENKRPETGWGEKISAYFSPEIQINNQAK
                     NGRSTKSFIQEGRLARLASQFQPNDFLFVQFGHNDQKIQEERGTLPFGSYLKNLQIFF
                     ECAKKANVQMVLLTSVTRRDYLENGTLNPDILGDYPKAMRAFAEKHHIPLLDVFSRSQ
                     ELFQTFSKEETKKFYLHLIPDTCKNYPEGLKDNTHFSPEGADKVARIIVELIKESRLP
                     LANYLQKGV"
     gene            6417..7253
                     /locus_tag="ACG8KR_000330"
     CDS             6417..7253
                     /locus_tag="ACG8KR_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFQ0199734.1"
                     /translation="MFPLNLGIRAHDLDVRNREEIVQKVSQLHLSHIQFAPQKAFPEQ
                     LKLTDMTLGSAAYFGEYFQKNNIELSILGCYINLSSKNQEVRAQALATFKQQIALCSA
                     YQAKMIATETGSVSVGYTKENFTEEAYQIARNSIIELVAHAENFGVTVAVEAGINHPI
                     YNYQLAKRLIDEVASPNLKIILDCANLMHKENHADQEKVIRGALDNLHGYITALHLKD
                     YIMENGKIRMVPVGKGCLDFRPVLHYIKYERPLMYATLEATPEIYVPEAIEHLQFIYQ
                     DC"
     gene            7264..9018
                     /locus_tag="ACG8KR_000331"
     CDS             7264..9018
                     /locus_tag="ACG8KR_000331"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0199735.1"
                     /translation="MEERIQKSYQKYHANAFQTLLHLYQGNYWRFAMSGLFFIIKHLP
                     SWLMPIAIANVINAASSKDSSQLQAIYFNAFLMACLIGQNILTNYFHVKYHSTSIRQV
                     EAGLRATLIKKIQELSIPYQKELQSGRIQSKIIRDVEAIQTLSSQVFVSGLNIAVNLF
                     VALGITLYKSPVVFMFFLLTVPLAAFVTLFFRKRIRQSNSDFRQEMEETSAKVIEVIE
                     LAPIARAHGLEKHETTRLNKHFTQIYEKGFHLDILQSVFGSVSWASFQIFQLICLIFT
                     GILALQGRIQPGDVVMYQTYFTTIVNSVSGMITLIPTISKGMESITSIGELLIAGDVE
                     TYHGKKKIQHLDGNFRFEDVSFLYPDSLKPIIDHFSLEVKAGETIAFVGPSGSGKSTL
                     LNLLIGFIQPTSGKIFLDDQPLDELDMRSVRNYLAVVPQTTLLFSASIKENITYGLKN
                     VSKERLDEVIEAAQLSSLIDQLPDGLDTLVGEHGNKLSGGQKQRISIARALIRQPKII
                     LLDEATSALDNQSEKKIQQALNYLTETPTTFIVAHRLSTIKEADKIVVIDEGKIVEIG
                     TYEKLLAQKGYFYRLKNE"
     gene            complement(9294..9635)
                     /locus_tag="ACG8KR_000332"
     CDS             complement(9294..9635)
                     /locus_tag="ACG8KR_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HFQ0199736.1"
                     /translation="MSNIYDSANQIEREIRELPEFKALKEAYEKVQANEEAHKLFKDF
                     QAMQIELQEKQMSGQEFSDEDAMKAQEMAVKIQSEEVINDLMQKEQGFSTIINDLNRI
                     IMTPVRDLYSE"
     gene            complement(9703..11874)
                     /locus_tag="ACG8KR_000333"
     CDS             complement(9703..11874)
                     /locus_tag="ACG8KR_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HFQ0199737.1"
                     /translation="MDFQSILEKIKAALISFWRWIRPYLGQFHRWRKRIWKKYHVNKL
                     ILLIGLICVLVTSIYLFILAKQANVETLKSGLSQSTVIYDKKNEEAGTLYAQKGTYVE
                     LNAISPYIQDAVISTEDRNFYEHHGFDIKGIARAAVRMVLSGGTSGGGGSTITQQLAK
                     NAYLTLDQTFDRKAKELFLAIEIEKKYSKEEILTMYLNNAYFGNGVWGVQDASRKYFG
                     VDATNVTLSEAATLAGMLKGPGIYNPIDHPKNANDRKNTVLSVMEENGKITKEQEQAE
                     SQTDISAYLNDTYDNSDSGYRYPYYFDAVIDEAVNEYGLDEEDVMNKGYKIYTALDQD
                     YQKQLEATYQNSALFPANASDGAMVQSASVALDPNTGGVQALVGRRGDHVFRGFSFAT
                     QMRRSPGSTIKPLSVYTPALEAGYKPSSILKDEPQSYYDAHNFDGTYQGEVPMYQAVA
                     QSLNLPTVWLLHEIGLQKGYDKAEEFGLPLAKSDKYYGLALGGLEKGVSPLIMAGAYS
                     AFANDGQMYTPHLITKIVDSTGAVIVDNTNKKPKQVISKETADEMTSMLLGTFSNGTA
                     AAANPAGYTIAGKTGTTETNFDATKANDQWMIGYTPDVVISTWMGYEESSKLHYLEGT
                     SGNVVGKVFKSAAEGILPYTSKTGFSVADAYATGGQVVAADQVGNTGNSNGESGNWQD
                     NLNQYGEQAKEGLKNFGDMVKEGVQGIGEAAKDLWRKYQGE"
     gene            12085..12942
                     /locus_tag="ACG8KR_000334"
     CDS             12085..12942
                     /locus_tag="ACG8KR_000334"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFQ0199738.1"
                     /translation="MKITITLPMNHPETTIRDLLEKEWLVPRKVRHFLRIRKNVWINQ
                     QPALFHHEVKGNDQITLLFEETDYNYQEVQLGDAKHIRVLYEDEHLIIVDKPAGMKTH
                     PNEPTENSTLLNHLAAYLDTKQQRPYVVHRLDKETSGAVLFAKNPFVLPILGRMLEQK
                     MIYRRYQAIVWGEIAKDMTITNKIGRDRHDRRKRVVDDKNGQSALTHVTVNKVLSEQT
                     QVYCVLETGRTHQIRVHLAHKGHPIVGDPLYQNRTAERLMLHALELHMIHPFTQEKIV
                     GKALPGLWE"
     gene            complement(13013..13870)
                     /locus_tag="ACG8KR_000335"
     CDS             complement(13013..13870)
                     /locus_tag="ACG8KR_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFQ0199739.1"
                     /translation="MYGGIEAGGTKFVCAVSDGGEILEKLSIPTTTPEETLDQVFEFF
                     DRYELKGIGIGSFGPIGIDKTKDNYGYVLATPKKGWAQFDFLGTIKERYNIPAAWTTD
                     VNAAAYGELKQGAAQGKNSCIYLTVGTGIGAGVVINEEIFSGIAHPEMGHIWVKRHPD
                     DHYEGTCPYHKDCLEGLAAGPSIEARTGIKGQDLPQDHPVWAIQAYYIAQALINYTLT
                     LAPEKIILGGGVMNQDHLLQKIRQQFVELMGGYMETPQVSEYIVRWGLPNESGIIGSL
                     LLAEHEHQK"
     gene            complement(13855..16557)
                     /locus_tag="ACG8KR_000336"
     CDS             complement(13855..16557)
                     /locus_tag="ACG8KR_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HFQ0199740.1"
                     /translation="MKKKVYIISHSHWDREWYMAYEQHHMRLIELIDDLLELFEVDPS
                     FNSFHLDGQTIILDDYLQVRPEKRQAIQQAINEGKLRIGPFYILQDDFLISSESNVRN
                     MLIGMEESRKWGTPVMLGYFPDTFGNMGQTPQLMKQAGISAAAFGRGVKPIGFDNQVL
                     EAENYSSQYSEMWWKGPDQTAIFGLLFANWYSNGNEIPVEKEAALAFWKQKLADAEQY
                     ASTNHLLMMNGVDHQPVQKDISKAIHLANELFPDYEFIHSNFTDYLEAVQKDVPEDLG
                     SVEGELTSQETDGWYTLANTASARVYLKQWNTKVQRQLENITEPLATMAYEVSGNYPH
                     DQLDYAWKTLMQNHPHDSICGCSVDSVHREMIPRFEKADEVGKYLAQDSLEQLTAAID
                     TTGFPKDSFPFVIVNTAGMDKTGEAEITIELERKRFAEGIPEQLYQELENLPKRKYHV
                     ETKSGATIPAILSEETVQFGYDLPKDRFRVPYMARMIKVTLPLENMPAFSWETFALVE
                     GEAKAEEKETMIHQSGRIIENGPLHLTIEKNGTITMEDRKNKRKLNNLHIFEDIGDIG
                     NEYIFKQPFCDQPILSSNKENSEVKVLVDTPEIAKISILQEMEIPVSADERLEKEQQM
                     VVEFRYRKAERSKEKRILQIKTIMTIRKDSKKIDFETTLDNQMKDHRLRVLFPTKLHV
                     EHHEADSIFEVVKRPNHVSKSWENPTNPQHQQAFVNIHDEEYGVTVGNFGLNEYEVTE
                     DGQIAVTLLRSVGELGDWGYFPTPEAQCLGEHRFNYSIELHGPEEKFSTYLHAYAAQI
                     PFSTQQIKHHEGTLISKQQYLTIKSETFAITALKRSKFSDKVVVRGFNMSSHLEKLEI
                     TKDNGKTVILNLLEEPTKQAVVPIIQPYEIRTIGFEEENECMAE"
     gene            complement(16570..17859)
                     /locus_tag="ACG8KR_000337"
     CDS             complement(16570..17859)
                     /locus_tag="ACG8KR_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HFQ0199741.1"
                     /translation="MAYKDIPKSVQKFMEDITQKCGDIHADWAKNFTAAFANTLLTTV
                     KKQNDGTTFLLTGDIPAMWLRDSTAQVRPYLVIAKEDEELADMISGLVKKQFFYINID
                     PYANAFNEEANGAGHQTDHTKMNDWIWERKYEIDSLCYPIQLAYLLYKNTGKTDQFNQ
                     SFIEGIKKILDVFKTEQDHEHSPYTFVRDTTRKEDTLINDGRGAKTAFTGMTWSGFRP
                     SDDTCRYGYLVPSNMFAVVVLGYVEEIFQTILDDKEIVQQTQKLKKEIQEGIQNHGRT
                     VNQKGQTIYAYEVDGMGNATVMDDSNVPNLIAAPYLGYCSEQEEDYLQTRRTLLSKEN
                     PYFYEGKYAKGIGSSHTPEKYVWPIALAMEGMTTSDKSEKERILNLLVATDAGTHLMH
                     EGFDVNNPENYTREWFSWANMMFCELVMDYFDIRVEK"
     gene            18009..19055
                     /locus_tag="ACG8KR_000338"
     CDS             18009..19055
                     /locus_tag="ACG8KR_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFQ0199742.1"
                     /translation="MNQPLYQTIFNDLQTAILTGELPVDSQLPTEKELSRTYKVSRIT
                     SKRALTELEQLGLIYRVRGKGSFVKSASPEQILASPTKTRRILFLLPYVADLSVGDFS
                     KGLNPVMQDKQIDVMMTTLDFLQNKTAKELINEFDGLIYYALDTEQHLDLLFELSLKE
                     FPVVILDKKIYELPFPTVLSDNFQGGALAASYLIEQGHSKIAYLFGTQTHPQSVRQRY
                     LGYLEALDKAGLTFHTSLDDKSATLDELLSYITKYEVTAFVCENDVVAIQTMNLLRQH
                     GYHIPEDFSIIGFDDIQAAALVDPPLTTIAQDFEQLGKLAGDALVTWLETKDRPSDSK
                     FPVSFIKRQSTKEK"
     gene            19057..21210
                     /locus_tag="ACG8KR_000339"
     CDS             19057..21210
                     /locus_tag="ACG8KR_000339"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HFQ0199743.1"
                     /translation="MNIGQIDTRHATANQPNFSNGNCQPYTGVPFGMNYFAPQTTDQN
                     GSWWFHPDDRVFQGYRLTHQPSPWMGDFSHMLFTPINGKLQENTLFHAQSSYRPEESI
                     FCPTHLSICQLRYGICSTLIPSMYGGILSIDYSKNDSGLLLSFPGRHQLFVIDPYTVE
                     GKITHFAGSEDPDFSFYFILRFEYPLTIDELAVSNKNSDSIPLYFEQTKKQTIRFGTS
                     FISSEQAAYNLELEQNQSKETYLAHSQEQWQSYFDRIQLTHHDQQQLRTFYHNLYRVF
                     LFPQTFYEIDPSGQKIHYDTTSRSIKPGVLYTNNGFWDTYKTVYPLYSLIAVEKYEEM
                     LEGFLNSYRESGYLPKWLSPDERGLMPGTLIDAVIADAAVKDIRRDLMPEFLTAMEKG
                     ATVQSKHPNYGRQGTEDYLTYGYVPNHYHESVNHTLDYAYSDFCISQVAKVLEEEEMT
                     KKYQQQSLNYQNIFDYESGFMRSKDKDGKFREPFSANRWGQDYAEGSAWQSSFAVYHD
                     FSGLIGKYGGSERYKEKLIELCNQPPTFDPGGYGFEIHEMSEMAAIEFGQLAISNQPS
                     FHYPYLFSYIGQPEMAQPLLKQLMTQTFDDSPMGYPGDEDNGSMAAWYIFNSLGFYPV
                     TPGTGEYVIGMPLIDKAVLLLSNGKELTIEATPNRPQQQFIHKIKRMDKEYTKLYFTH
                     QDLLKGGTISYQLGIIPHPKKYQAKDLPFSLSNYN"
     gene            complement(21683..23134)
                     /locus_tag="ACG8KR_000340"
     CDS             complement(21683..23134)
                     /locus_tag="ACG8KR_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFQ0199744.1"
                     /translation="MSVYQVLLVDDEYMILNGLKKIIDWQSLGFQIVATAENAKEGLA
                     VLEQRQIDLVVTDVTMPEINGLEFIEAAQKERHNFEFMILSGYQEFDYLKGGMQLGAV
                     NYLMKPVNKFELVESLKKIKTRLDQQNEQKNQQEIYQELLFSQWLNEELDEISEEEII
                     GQISEKQHRIVLIQLARTHDTLINRWLQERQQIFYYQRNYGDRVLITLLLEADEVDGF
                     CCFVQENFPDQEWLISIGEETDSLDKIPETFQQAKDNLQLHQFYGDKDEHVFYAVQAE
                     TKDQTIDFSSFRRVLQNKQLEVAQKMITDFFEQFQLAAMMPEDIRYSSFLLFMEIQRE
                     LIDLEDEEYLQGIEKIHQAKTVQELQQLLLSFIKKHQRQKKYSDNVEKVIVILHQHYQ
                     EPLTLKEVSENLHLNVMYLGQLFKKETKKSFSAYLNHLRMEKAKQLLLHSNQNINEIA
                     SEIGYNNTTYFSKLFKKIVGRSPKEYRENSGES"
     gene            complement(23131..24855)
                     /locus_tag="ACG8KR_000341"
     CDS             complement(23131..24855)
                     /locus_tag="ACG8KR_000341"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFQ0199745.1"
                     /translation="MNKFRILFSKGTLLNRLLRKYAVIMIGVTMTATVIFSIHTWEQN
                     QKQAENMTSDAVQSTSRMLNDKTTLSRIIKNQLVGNSEKIENVTTYLTKPIDQYLMYV
                     YEQQNSTDELVSFPNQIKDLYANYEELSAIYIVLNQLPEYYESSRENKGGEIKKGTPK
                     LLDAFYIKLPITQGGAESGSIFIGFQKKELDMILENLTSFNGLSLYMISGTTNRLYTF
                     HDNKIAKETFERQEMIITESLKKNSQLPIDDLEKNNFVQHQELSDDYRILAVLDKKSV
                     KIETGKNLAPLIIGIIILDNILLIFLYKTFKRYSQQVSIVMQAMDQTAAGNLETRIDI
                     TNTEYELKELSIGINEMLDSINQFVEDIYKLEIKQQDAHMRALQAQINPHFLYNTLEY
                     IRMYAISEGSEELADVVYAFSALLRNNTNQEKTITLKEELDFCEKYVYLYQMRYPNRV
                     AYHFMIDPDLEKIEVPKFVIQPLVENYFKHGIDFTRFDNALSVKVLQEGKRVRIIIKD
                     NGKGMTEKRLKQIEEKLSHPKVELHGSIGLQNVNERLRASFGSSYYMSLENNETGGLT
                     VSITFKEG"
     gene            complement(24848..25462)
                     /locus_tag="ACG8KR_000342"
     CDS             complement(24848..25462)
                     /locus_tag="ACG8KR_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HFQ0199746.1"
                     /translation="MLGKVLEELFIRIWVIIKLTLYFWIYTFAGGIIFGLGAAWKTVN
                     ELFYLYGFEYKEITIKRGWNIYKRNFLRGNLLFSLFLSGTALLSYNLYLSVQIKGLIF
                     LAIDFILLFALLCLFATYQYSMILDSEYTISVPNLLKLSFISVFSSFSSFLKIIIGSG
                     IILGVTWQFKGLILFGVIGLLTVWNGTMTTHWREELDKQLESYE"
     gene            complement(25807..27264)
                     /locus_tag="ACG8KR_000343"
     CDS             complement(25807..27264)
                     /locus_tag="ACG8KR_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFQ0199747.1"
                     /translation="MKLWKKLAFTGVSALLLGTLAACGGSGSKDQAEASNSDTLQMYQ
                     IGDKPDNFDQLMEVANKRIEKEIGVKVNINYIGWGDYEKKMNVIISSGENYDIAFANN
                     YVSNAQKGAFADLTELAPKYAEKAYNDLDEAYIKGNLVNGKLYAFPVNGNVFAQQVLT
                     FNKALLDKYDLSIDGIQSYADAEKVLKEFHEKEPNTAAFAIGQGFKVQGNFDYPLGNT
                     LPFAIDLDGDDSKIINQYDNETYIGLLRTMHSWYKQGLIPSDAATSNKDYPLEGNTWL
                     MRGETQGPYDYGDTILTNAAKQELVSKAITVPLKSTAQAQMANFVVSNTSKNKEKSVE
                     LLGLLNSDPELLNGLVWGIEGEAWEKVDGSDDKIKLLDGYQPNTHMSAWNTGNNKILY
                     TQESITDDMIAKRDQSIADAETSPILGFSFNTDSVKTELSNISNVMNQYLDGLNTGTV
                     DPDETLPKLKDALDKAGYDKVLKEMQKQYDEFRQE"
     gene            complement(27305..28225)
                     /locus_tag="ACG8KR_000344"
     CDS             complement(27305..28225)
                     /locus_tag="ACG8KR_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0199748.1"
                     /translation="MKKKKVERVQVRSFNKTANFFSNIMIALFAISCILPFIFVIAIS
                     FTDESILSTHGYSFWPEKFSIFGYTYLFDQMQDKIFQALFVTVLVTVLGTLINSTATS
                     LYAYAISRSNFPFRRFFTVFCLITMLFSPGMVANYLVMTNLLQLKDTIWALILPMAVS
                     PFNIIVMRTFFKRSVSDSIIESARIDGASELRIFVQIVLPLAVPGIATISLFAALGYW
                     NDWFNALLYIQDDKLVPLQYLLMKIQSNIQYLTQNSGAGSQLTGGLASVPGESARMAI
                     VVISTLPIAISYPFFQKHFVKGLTIGGVKE"
     gene            complement(28237..29184)
                     /locus_tag="ACG8KR_000345"
     CDS             complement(28237..29184)
                     /locus_tag="ACG8KR_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0199749.1"
                     /translation="MKKRKGFNHFFMNLWRYKALVLMAVPGMLWMIFFFYIPVMANVV
                     AFKNFHISADGFLASLRESPWVGWENFKFLFSSDQAFLITKNTILYNVAFILLNLLIS
                     VVFAIIMSELRNKRLVKVYQTMSLLPYFLSWVIISYFVYAFLSPDKGIFNQWITGHGG
                     EAVNWYNEPKYWPFILVFIGTWKGIGYNSIIYFASVMGIDPTYYEAAMVDGASKWQQI
                     KHVTIPQLIPLMTILTILAVGNIFRADFGLFYNIPRNSGALYDVTQVLDTYIYNGLTS
                     TGDFGMTAAAGLYQSVVGFVLLMITNTIARRFDSDSALF"
     gene            complement(29663..30382)
                     /locus_tag="ACG8KR_000346"
     CDS             complement(29663..30382)
                     /locus_tag="ACG8KR_000346"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HFQ0199750.1"
                     /translation="MRLDKFLADMGLGSRKEVKGLIKKGTIEVNGQVVKSDKAQVNEF
                     EDAVTYLGEIITYQKDFYYILHKPAGVISATQDNHDQTVIDLLEDQDYREDLFPVGRL
                     DKDTEGLLILTNDGVFAHQLLSPKKHVEKEYLAEVSGIMTEEDIRLFAEGLIIDGDEQ
                     TLPAKLQIDQVDQETETSIIRLILHEGKFHQVKRMVKAVGKEVLYLKRIRMGKFWLPK
                     DLNKGQYRPMTKEELEQVREK"
     gene            complement(30431..32077)
                     /locus_tag="ACG8KR_000347"
     CDS             complement(30431..32077)
                     /locus_tag="ACG8KR_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HFQ0199751.1"
                     /translation="MDNQRKPSLDQLTPQEKMARGSTWMTASNIISRLLGAVYIIPWY
                     AWMGENAKAANGLFNMGYNIYALFLLISTAGIPAAIAKQTARYNSLNEYGTSRRLFIR
                     ALQMMGGLGLLFALFMYIASPWLAHASGGGEELIPTMRSLSIAVLVFPCMSVIRGYLQ
                     GNQEMMPYALSQIVEQVARIFYMLLATFIIMKVMDGDYVTAVTQSTFAAFIGMLASIL
                     VLLYFLKKHQAYTSAFIHYSENKINIATKELLLDTVKEAIPFIIVGSGVTIFKLVDQF
                     TFMKIMSASTEYSNAQLLDLFSIFSANPDKLTMVVIALATSIASTGLPLITEAVTLKD
                     RVGLAKLTSGNLQLFSFFMFPATFGVMLLAYPLNTLFYTPDSLGSNVLIQASFVGLFL
                     GLYMLVSNMLQGMFENKAAINYLVIGFLVKLLLQYPAIRLFEVYGPLLATMIGFAVSC
                     TLILKRIHEVARFKPSFVWRRTLLIFILTLIMLLASWITKMIFGAFLNEDSKFQSLLL
                     ILVVAGIGALVYGYLALKIRLAERLLGPGIARLRHKLKIK"
     gene            32256..32846
                     /locus_tag="ACG8KR_000348"
     CDS             32256..32846
                     /locus_tag="ACG8KR_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFQ0199752.1"
                     /translation="MTQEIKKLPVIFLGLFFITVGLNWFLLPHDIASAGVGAIGHLVE
                     TAFSIQRSVTVWSINLMMLLLAAVLLGKSVFFKTVAGSLLFPVVLEIVPKVEMISSHF
                     FSLISGSLLFSLGVYTLYTVGASNGGITIPPIIFHKFFRLPMPQGLLLTNSLIVFLNY
                     YVFGLLETLFVLLSITVSSLFMKILIRLSPVSRKFE"
     gene            32938..34443
                     /locus_tag="ACG8KR_000349"
     CDS             32938..34443
                     /locus_tag="ACG8KR_000349"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HFQ0199753.1"
                     /translation="MSFSLEDIRHLLLKENLLKEFVSTRGWHLDVPEKAEFLQLSYDS
                     RKADASTLFFCKGMNFKEEYLDSAIEQGIQYYVSEQPYENSAVGIIVTDIRKAMALLA
                     MAFYDYPQNKLKVIGFTGTKGKTTAAYFTKAILDHTTNKKTALLSTMNTTLDGKTYFK
                     SHLTTPESLDLYRMMSEAVENGMTHLVMEVSSQAYKTQRVYGLTLDVGIFLNISPDHI
                     SPIEHPTFDDYFYCKRQLILNSKTVVLNHESDYFDLLRETADLFQIPTITYGRSEESN
                     YQVIRSDKGTHGFTLSSHEDRLAICDTAYDILLAGGFNQENAASAIIAAALTGASSQD
                     AQDGLKEARVPGRMDQLIQSNGAHVYVDYAHNYLSLKTLLKFAKNEHPDGRVIVVLGS
                     PGNKAISRRHDFGKVLSETADIAFLTADDPAFEDPKKIAEEINEAITNPDLIVHYEMD
                     RPEAIRRALAESAPQDSVVIAGKGVDPYQKINGVDEPYEGDYAIVKRLIEE"
     gene            complement(34527..35879)
                     /gene="celB"
                     /locus_tag="ACG8KR_000350"
     CDS             complement(34527..35879)
                     /gene="celB"
                     /locus_tag="ACG8KR_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HFQ0199754.1"
                     /translation="MFDFLQKYLMGPMGKIAQLKIVRAVMAAGMASIPFTIVGSMFLV
                     LNILPLPFPFLEGFFNATFFKVSDLYMIVNTMTMGILSIYFAIVFAYELTSIERDEQK
                     LNVNPLTGALLSVFAFFMCIPELIMTDGKISLIASINETETVVNGIRMGSFVERLGTS
                     GIFTAIIMSIIAVELYCMCVKRNWVIKMPDVVPPGVSRSFTALIPTFVIAFVVMIING
                     LLVALGTDIFKMIAIPFGFVTQLTNTWLGVMVIYFLIHALWIVGIHGANIITSFLTPI
                     VLANMAANAQGANYPLAGEFNNSYVTVGGSGATLGLIIFIAFLAKSDQLKVLGKASLV
                     PGLFNINEPIIFGMPIVYNPYLAVPFFLAPMASATLAYFAIRLEIVKPMLAQMPWPSP
                     VGIGAFVGSGGDWKAAVLAVICAIIAFIIWFPFIKFYDNKLLNEEREKAAELASEKLK
                     "
     gene            complement(35879..36397)
                     /locus_tag="ACG8KR_000351"
     CDS             complement(35879..36397)
                     /locus_tag="ACG8KR_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0199755.1"
                     /translation="MQDTVKRKIELEKKQLSLKNMYFNRYLIVRYLTAFFFFMNMQWM
                     ILLLSAKSLGSSLPMILILAILPAVGEQVKLYRKHQTNVPWTKWYFLFQSVFNLLVIP
                     VLFTSGFTFLYPFMANNNGGQLFVFILVVSGIFASAFIQYRLKKISLNQDQQYIRIKQ
                     YEKALYLGKENN"
     gene            complement(36413..36739)
                     /locus_tag="ACG8KR_000352"
     CDS             complement(36413..36739)
                     /locus_tag="ACG8KR_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HFQ0199756.1"
                     /translation="MAEKINSETLQVTSFDIIFHSGNARTLIHEAFRSMRKEMFEEVE
                     EKLRQANEEILHAHQSQTQLLKEYASGTKIEMEIIMVHAQDHLMTTMTLLEVAQEMNY
                     LYQKNK"
     gene            complement(36752..38731)
                     /locus_tag="ACG8KR_000353"
     CDS             complement(36752..38731)
                     /locus_tag="ACG8KR_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFQ0199757.1"
                     /translation="MKQKEKLLLRYLIDHKKEYVTSQRLASELLLSDRTIRNYLQRIK
                     ELVEKNGGKIIAKPGYGYQLHILQRLTFDLFLSRQEIVPGYAREPQGFYESEDRQKYI
                     LNQLLLEDAILFMDDLAEELYISRSSLTKDMQEIKERLIPYSLKIVSKHGQGTWIEGE
                     ERNRRHFIMDTFFGNHYGNSLKEYLGQSRFFPDISFEELVIIILDETREAKLKVSDFI
                     IQNLTLHLALGIKRLREGFEIKELGIEQEVSKRVEYQVAQRIVQRIEVIAGVSFPEEE
                     IAYLTLHLMAKSNHKENQNDQELSKELAKVLKRLSQEFHFSLVEDLQLFNGLLDHLSP
                     MMIRLSRGIRIENPLTEEIKKENPKVFDAVKRHFSNMPALKNYTINEDEWAYLALHLM
                     AALEKERAAHKLHALIICATGYGSAQLLKNRVVSEFGKNITVVSVKGYYEINETTLKG
                     IDLIISSIDLSTMFFKIPVLHVSIFLNEDDVRKIRKVVGESIPYHSSDRPIPAFSLQQ
                     KKQIYTEQLSEKFFKTYTYEPKKEEILHDLLQKLSINEEEGYIREMRKQIQQRENMGQ
                     IIFSDTVAVPHPAVPVGIETKVAVALIPSGMQWDQYQEIHFVFLVSPSYVENEGITVV
                     TKAIVRLVDQLKVQQEILAEPIFENFSSKFIKLIE"
     gene            complement(38752..39066)
                     /locus_tag="ACG8KR_000354"
     CDS             complement(38752..39066)
                     /locus_tag="ACG8KR_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HFQ0199758.1"
                     /translation="MKKALIICAAGMSSSMMASKTTDFFKQKREEVLVDAVSATEGDN
                     MIKNSDFDLFLISPQTTMFLDKFKKLGESVGKPVVSIPFQAYVPIPTGIQKLAEVIEE
                     NI"
     gene            39234..39527
                     /locus_tag="ACG8KR_000355"
     CDS             39234..39527
                     /locus_tag="ACG8KR_000355"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase family
                     protein"
                     /protein_id="HFQ0199759.1"
                     /translation="MSITVNILYTSTNGQARKFAEEMVERNIVKAIRAEEGNEKYDYY
                     FPLEDPESLLLIDRWKDEEAIEKHHKSEMMAQINELRKKYKLKMKVERFTDLP"
     gene            complement(39605..40717)
                     /locus_tag="ACG8KR_000356"
     CDS             complement(39605..40717)
                     /locus_tag="ACG8KR_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFQ0199760.1"
                     /translation="MTVKNKSKKKGRQQVDFYQSLQLDPFILKQKIREAASKKEKCMY
                     ICSLFLRSLFIVLFAICFIIIITTLFESKHKPYAVVLFCMLMSIRFVDFGYKISHSII
                     GLAIVMFSLLVAPYVQLIKWSVMGMLIHFILLSSILMATASDPKMGNASLYGFSYLFI
                     VYSLPKDSLNKNFFTQTSSLLFLFFCWFSVLLYRKHREKNKDKSLFKEIFLKGMYSQE
                     KIWMLIYAFGISLLIVAGEYVPFQRLMWAGFAFSSIVSSYGLMSIGFKERAVDRIIGS
                     LIGCVLFIGISQFIPFNWVGILGGLALGVCSTYRYKTVFNSFGALAITASLFGVPGAV
                     TIRIFENILGVCLGIMYIGVTEILIRKIREKHGLNH"
     gene            40870..41718
                     /locus_tag="ACG8KR_000357"
                     /pseudo
     CDS             40870..41718
                     /locus_tag="ACG8KR_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(42071..>42461)
                     /locus_tag="ACG8KR_000358"
     CDS             complement(42071..>42461)
                     /locus_tag="ACG8KR_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFQ0199761.1"
                     /translation="LQGAVFELQDANGTVLQSGLTTGADGKLAIDGLAPGAYQLVETQ
                     APTGYELDATPVTFKIEKEQEVAIFLTKENRKVADSSDIRGNNPTSSGNKHLPKTGET
                     IISQFILSILGVLLVFISIKFRKKRNI"
BASE COUNT        12816 a         8554 c         7627 g        13464 t
ORIGIN      
        1 tcatctaatt aaatgagcat tccccgcttt caagaatagc ataattaaaa ataaaaatcc
       61 aagtaatttt taacgtgtgc aatcttttca tatcaaagat ctattgactt atcgtagtta
      121 tcgtaatatg atgaatgcgt taacaaaaag tgaaaaagta aggaaaatga tggaatggta
      181 acgatcaaag atgtcgctaa agcagctggc gtttctcata cgacagtttc tcgtgctttg
      241 aataacagca gtttaataaa aaatgaaaca aaacaaaaaa tacgacaact agctaaggaa
      301 atgaattatt cgccaaacta tagtgcaaaa ggtttagtga atcaaaaaaa atatctaatc
      361 ggactatttt tctcaagttt gcataaagga acttcatcaa atttcctagc agattccatt
      421 gcaggcatcc attcagtatt agacacaaat tacagtttat ctgttgaaag tatcgatgtt
      481 gttgatttaa cagaaatcaa cctgcaacgc tacgatggac tgatcgttat gagtcaaagt
      541 gatgatgatc agcctttcat cgactatctc aaagaaaaag aatttccttt cgttgttgtc
      601 aatcgtcaca tcaatgatcc agcggttatc aacgtcactg ccgatgatgc ggccggtgtt
      661 atgcaggcag tagattacgg tattggatgc ggacatacga agatcgctta tattggcgga
      721 aagaaaaatt ttcgttcgac aaaagaacgg gaaaaagggg tattgaaaag tctaaaaaaa
      781 gcgagcatcc ctgtcaatcc agcctatttt ttaacaggag attacagctt agaaagcggc
      841 ctccaaaaca tggaatacct attatcactt tctgacctgc ctactctggt tttttgcggc
      901 aacgacgaca tagcgattgg tgcattgaga actgtacatt tagctggcct ccgtgttccc
      961 cagaacattt ctctgatcgg gttcgatgat tctccagtcg tctcctatct caatccgccg
     1021 cttaccaccg tacgtaagcc tttgaaagaa atgtgtcaag aaggaacaaa gcttttacta
     1081 gatttgatcg aaggcaggac gatcgaacaa tctaaaattg agctctccac cttgttgatc
     1141 cttcgtgagt cgatcaatta cttgtaaact acaagtaatt ttatataaaa atgttaacgt
     1201 gtgcataaca cagaaaggaa gtttatatga aaaaactgac aaaagaatta gtgaaaaaaa
     1261 acgaagcacc tgtaaaagtt cttcagtttg gtgaaggcaa ttttatgcgt ggatttattg
     1321 attggcagat tcagcagctg aataaccaac aattgtttaa aggtaatgtt gcaatcattc
     1381 aacctttaga acaaggtctt ggaaatatga tgaaccagca agataattta tatacggtca
     1441 ttttacaagg actcagcaat ggcgaaatcg ttgatacttc cgagattatc accgtagtag
     1501 aacaaatgat caatccttat gatgaatggg atgcttatct atctctagca gaaaatgacg
     1561 acttggccta tatcgtctcc aatacaacag aggcaggaat ccaattcgtg gagactgata
     1621 atttaacaga tactccccca cagagcctcc cgggcaaact gacagccttt ttataccgac
     1681 gtttcaaatt aaacaaagct ggctttacca tcattccttg cgaactgatt gacagaaacg
     1741 gcgaacgtct aaaagaaatc attttacgct acgcagaaaa atgggattta gaacctgctt
     1801 ttgttgagtg gttagaaaaa gaaaatgttt tctgttgtag tttagttgat cgcatcgtcc
     1861 caggttatcc tcgcgaagat gcaggtgttt tcaatcaacg gcataactat gaagatcaat
     1921 tgatggtcaa agcagaacca ttcatgcttt gggtgatcga agggcctaaa gaattggaga
     1981 atcaactacc attcaaacag gctggcttga atgtcatcat cacagatgat atgactcctt
     2041 atcgtgaacg taaggtgcat ttgcttaatg gaccgcatac agcgatggtc cctcttgctt
     2101 tgttggcgga attggaaact gtagaagaag taatgaaaga tcctttattc tccgcatatg
     2161 ttgatcagtt attcaatcta gaattgattc ctatgctttc tttacctaaa gatgaattag
     2221 ccatttatgc tgaccaaatc aaagaacgtt tcttgaatcc ttttgcccat cacaaattag
     2281 aagcgatttc attaaatagt gtttcaaagt tcagcacccg tctgcttcca gtgttcaaga
     2341 agtatatcga agaacaaaat caggtaccac cgttaatcac tgtttcacta gctgcgctac
     2401 ttcttatgta tcgaggcgat caagtaaagc cacatgatga tgaaaagacg ataagcgaat
     2461 ttactgatgc ttggtctgat aatggaacag ctattcctcg attattgcaa aacgctgcgc
     2521 tttggggaga agatttgtct caaataccga atgtgacaga tacagttcaa gaaatagctg
     2581 caaagatcga tcaaaaaggt gtccggtcag tattacaaga aatgaaagga acgatggtcc
     2641 atgcttgaaa ccctgcaacc aacaatgaaa ttaaatcgac gagacagtgt ggtcgtcgca
     2701 ttaaccccta ttccaaaaca aacacaactg acgattgatc agcaaacgat caccactctt
     2761 gaagatatcc cgcaaggtca taagattgca ttgggtgatt tgaaagctgg agacaacgtg
     2821 atcaaatatg gctatccaat cggtcatgtg acgacagacg taactgccgg tcaatggctg
     2881 cacactcata atgtaaaaac caatctttct ggtgaattgg attatcgata tgaaaaagat
     2941 gtccatccaa gtcattacct ttttgagaac cgaacatttc aaggttactt gcgaaaaaat
     3001 ggaaaagtag ggattcgtaa tgatttgttt ttagtaccta ccgttgggtg tgtgaatggc
     3061 attgctgaac tgatcgtcaa acagttcaag gaaaaacatc cagatctggg acagtttgac
     3121 catattacga ttttaaagca tccttatggc tgttcgcagc ttggaaaaga tcatcaaaat
     3181 actcgagaaa ttttagcgga cgcagtaaat catcccaatg ccggaggagt tttagtattt
     3241 ggattaggtt gtgagaacaa tacagttcct gaattcaaaa aaattttagg tgaatatgat
     3301 gaagaacgag tgaaatttct agtggctcaa gatgtttacg atgagatcga acaaggtgtg
     3361 gctctattag aagagctttt aacagcggcg gaaaatgacc agcgcatatc agtcccgctc
     3421 tctaagttga atgtcggttt gaaatgcggc ggttctgatg gtctatcggg aataactgct
     3481 aatcctttac ttggtgcatt ttctgactac ttgattgctc aaggaggcag cacgatctta
     3541 acagaagtgc cagaaatgtt tggcgcagaa caagtattga tggcacgagc ggaaaatgaa
     3601 gaagtttttg actcaatcgt tgacttgatc aatgacttca aacagtattt cttgtcttac
     3661 ggggaaccag tttacgaaaa cccatctcct ggtaataaag caggtgggat cactacatta
     3721 gaagataagt ctttaggctg cacgcaaaaa tcaggaagct ctcctgtcgt tgatgtgcta
     3781 caatatggcg aaaaaatcag aaaaccaggg cttagtttat tgcaagctcc cgggaatgat
     3841 cttgtagctg cctctgctct tgcttcatcc gattgccagt tggtcttgtt taccacagga
     3901 agagggactc cgtttggctc atatgtccca acggtcaagg tatctacgaa tacaactctt
     3961 tttgaacgca aaggtcattg gatggatttt aatgcaggcg tcttattaga acaaccaatg
     4021 gaagtaatct tagaatcgtt tgttcaaaaa atcatcgctg tagctagtgg agaagaaacg
     4081 aaaaatgaac aaaatgacgt tcgagaaatt gccattttta aaaacggagt aacactttga
     4141 ttttcataaa acagtataaa aagaaagaag gaagatttat gtttttatct gatgattttt
     4201 tattaaaaga cgattgggcg aaaaagcttt accattccta cgcaaaaaac atgcccatta
     4261 ttgattatca ctgtcactta gatccaaaag aaatttacga aaacaacaat ttcagcaatc
     4321 tgacagaagc ttggttagga ggcgatcatt acaaatggcg cttgatgcga gcttgcggcg
     4381 tgcctgaaga aaaaatcaca ggaaatgctt ctgattttga aaaattttta gcttggtgtc
     4441 aaaccgtacc aaaaattatc ggaaatccgc tttacagttg gtcacatctt gaattgaagc
     4501 gtttcttcgg tattgacttg ttgataaatg aagaaaatgc agagaaaatt tggacacaag
     4561 caaatgaaat tttggcagct cctgattttc gccgtcggga aatcgcaaaa aaatcgaatg
     4621 tagaagtgat ttgtacaaca gatgatcccg cagacgactt gcactatcat cagttgcttg
     4681 ctcatgaaga accagatttg aaggttctac cttcattccg tccagataaa gcactcaata
     4741 ttgaaaaaga aggatttaat gcatgggtca cactactgga agaagcagct gaccaaacga
     4801 tcaccaccta tcaagaactg atagaagtct tagggcagcg gatagaatac ttccatcaac
     4861 atggatgtcg tgtttccgac cacgctttag acatcttacg ctataaagaa gcggatgaaa
     4921 cagtattaga agagattttt caaaaagcac gagctaacaa actattatct tcagatgaaa
     4981 tcgatgctta tcgtacagaa acactgatac atttgaccca cttctatcat tctcataatt
     5041 ggacgatgca gctgcacatc catgcttatc gtaactgtaa tacacaaatg ttggaaaaat
     5101 tagggcccga taccggctat gatggaatga atgatctttc tttgactatt cctctgcaaa
     5161 aattattgaa ccgtgcagaa gaaacggatc agttgccaaa gacgatcctt tattcattga
     5221 atccgaatga ctatcctgcg attttagctt tgatgggctg tttccaaaaa gaacagaatg
     5281 gaaaactgca gttaggttct ggatggtggt acaacgatac tcgagctggt atgcgcgatc
     5341 aattgacaca atttgctgat ggtagtgctt tggggaattt tgtcggcatg ctgacagatt
     5401 caagaagttt cctgtcctac actagacatg aatatttccg tcgtgttctt tgtgaatttt
     5461 taggcgaaat ggtcatgcga ggagaagcac ctgaagatga acaactttta ggaagtctag
     5521 ttcaagcgat tagttataca aatgctaaaa attactttgg cttttttgcc taattgatta
     5581 atagtaacag taacgaatca aaaacaggat agtgaaataa cggaatccgt ttatccacgt
     5641 tttagagaag aagacggacc aaatttgctc cgtcttcttc tcttgctgtt ctgcttcatt
     5701 gcctataaac ctttatgaaa gaagagataa catgcctact atccatattg ccggtgattc
     5761 tactgcggca gaaaaagatg aaaacaagcg tcccgagaca ggttggggag aaaaaatctc
     5821 tgcttatttt tcacccgaga tccagatcaa taaccaagca aagaatggac gcagcaccaa
     5881 atcatttatc caggaaggaa ggttagcccg tctagcttca cagtttcagc cgaatgattt
     5941 cttatttgta caatttggac ataacgacca gaaaatccaa gaagaaagag gtacgcttcc
     6001 cttcggtagc tatttaaaaa atctgcagat attttttgaa tgcgcaaaaa aagcgaacgt
     6061 ccaaatggtc ttactgacat ctgtgacgcg acgagattac ttagaaaacg gaacattgaa
     6121 tccagatata cttggagact atccaaaagc gatgcgtgca tttgctgaga aacaccatat
     6181 tcctctttta gatgtgttct caagatcaca agaattgttc cagaccttct caaaagaaga
     6241 gacaaaaaaa ttctacctgc atcttatacc tgatacttgt aaaaattacc cagaaggact
     6301 aaaagacaat acccacttta gtccagaagg cgctgataaa gttgcccgaa taattgttga
     6361 attgataaaa gaatcccgtc tgcctcttgc taattattta caaaaaggag tttgagatgt
     6421 ttccattaaa tttaggcatc cgtgcccacg atttagatgt tcgaaatcgt gaagaaatcg
     6481 ttcaaaaagt atcacagctt cacttgtccc atatccaatt tgcaccacaa aaagcatttc
     6541 ctgaacaact taaattaact gacatgacac ttgggagtgc tgcctatttt ggtgaatact
     6601 tccaaaaaaa caatatcgaa ctttccattt taggatgcta tatcaaccta tccagcaaaa
     6661 atcaagaagt tcgtgcacaa gcattggcaa ctttcaaaca gcaaattgct ctgtgctctg
     6721 cctatcaagc caagatgatt gctacggaaa ccggtagtgt ctctgtcggt tacacaaaag
     6781 aaaattttac tgaagaagct tatcagatcg ccagaaattc tatcattgaa ttagtcgctc
     6841 atgcagaaaa ttttggcgta actgttgctg ttgaagcagg gatcaatcat ccaatctata
     6901 actatcagtt agctaaacgt ttaatcgatg aagtagcttc accaaattta aaaatcattt
     6961 tagattgtgc gaatttgatg cataaagaaa atcatgctga tcaggaaaaa gtgatccgag
     7021 gtgccttgga caatttacat ggttatatta cagcgcttca tctcaaagac tatatcatgg
     7081 aaaatggaaa gatccgcatg gtacctgttg gcaagggctg tttagatttc cgcccggtac
     7141 tgcattatat caaatatgag cgtcctctaa tgtacgccac tcttgaagct actcctgaga
     7201 tttatgttcc tgaagcaata gaacacttgc agtttattta ccaagattgt taaggagaag
     7261 attatggagg aacgaataca gaaaagttat caaaaatatc atgcaaatgc ttttcagaca
     7321 ttgctgcatc tttatcaggg aaattattgg cgttttgcca tgtcaggact cttttttatt
     7381 atcaaacatc ttccatcttg gttaatgccg atcgctattg ccaatgtaat caatgcagct
     7441 tcatcgaaag attcttctca gttgcaggcc atttatttca acgctttttt gatggcttgt
     7501 ctgattggtc aaaatatatt gacgaattat tttcatgtaa aataccatag cacttcgatc
     7561 cgtcaagtag aagctggact aagagcgacc cttatcaaga aaatccaaga actatctatt
     7621 ccttatcaaa aagaattgca atctggacga atccagtcaa agatcatccg tgatgtagaa
     7681 gctatccaaa cgttgtcctc tcaagtattt gtcagcggat taaatatcgc tgtcaattta
     7741 ttcgtagctt taggtatcac gctttataaa agtcctgttg ttttcatgtt tttcttattg
     7801 actgttcctc tcgccgcatt cgttactctc tttttccgta aaaggatcag acagtccaac
     7861 tcagacttca ggcaagaaat ggaagagact tctgcaaaag tgatcgaagt gatcgaatta
     7921 gcaccgattg ctcgagctca tgggttagaa aaacacgaaa caactcgctt aaataagcat
     7981 tttactcaaa tctatgaaaa aggattccac ttagatatcc tgcaatctgt tttcggatca
     8041 gtcagctggg ctagttttca gatatttcag ctgatttgtt tgattttcac tgggatatta
     8101 gctttacaag ggcgcatcca acccggcgat gtcgtgatgt atcagacgta tttcacgacg
     8161 atcgtcaatt ccgtttctgg aatgatcacg ctgattccta cgatttcaaa aggcatggaa
     8221 tctatcactt ctattggcga actgttgatt gctggcgatg tagaaactta tcacggtaag
     8281 aaaaaaatcc aacatttgga tggaaatttt cgttttgagg atgtttcttt tctttatccg
     8341 gactcactca aaccgattat cgatcatttt tctctagagg tgaaagctgg agaaacaatt
     8401 gcttttgttg ggccatctgg ctctggaaaa tctacactcc ttaacttatt gattggattt
     8461 atccagccga cttcaggaaa aatttttcta gatgatcagc ctttggatga actggatatg
     8521 cgaagtgtgc gaaattattt agccgttgtt cctcaaacca ctttgttgtt ctccgcttcg
     8581 atcaaagaga acatcactta tggcttgaaa aacgtatcaa aagaacgttt agatgaagtc
     8641 atcgaagctg cgcaactttc ttcactgatt gaccagcttc ctgatggtct ggatacctta
     8701 gtaggcgaac atggcaacaa gctgtctggt ggccaaaagc aacgtatctc cattgcacga
     8761 gcgttgatac gtcagccaaa aatcattttg ctagatgaag caacctcagc tttagataac
     8821 cagtcagaga aaaaaatcca gcaagccctg aattacctga cagaaacacc cactactttt
     8881 attgtggctc atcggctttc aacgatcaaa gaggcagata aaatcgttgt gatcgatgaa
     8941 ggaaaaatcg ttgaaatagg tacttacgaa aaactgttag ctcaaaaagg ctatttctac
     9001 cgcttgaaaa atgaatgaca aaaaatagga gctatgacaa gacttttgtc gtagctccta
     9061 tttctgaata aacggtgttc tcctgccaac tcctcggtca caagtcacaa tagtcatcaa
     9121 acatgagaag ctgttttttg aaattgttcc ttgtaactca gagttaaacg gtaggctcac
     9181 gacctcttgg taaatggtgt tcaagagaca gcctatcggc aataagaagc aattttgtca
     9241 tcgctcaagg ccgaacgtct ctttcacacc ctctgttgtt tcataaaata gttttattcg
     9301 ctgtaaaggt cgcgaactgg tgtcatgatg atacggttca agtcattgat gattgtactg
     9361 aagccttgct ctttttgcat caagtcattg attacttctt ctgattggat tttcacagcc
     9421 atttcttgtg ctttcattgc atcttcgtcg ctgaactctt gtccactcat ttgtttttct
     9481 tgtaattcga tttgcatcgc ttggaagtct ttgaacagtt tgtgtgcttc ttcgtttgct
     9541 tgtactttct cgtacgcttc tttcaacgct ttgaattctg gtaattcacg gatctcacgt
     9601 tcgatttgat tcgcgctatc gtagatgttt gacatgttat gccctctttt cttttagtag
     9661 ttactctcta ttgtaacatg gggttattcg ttttcaaagg ggctactctc cttgatattt
     9721 ccgccagagg tctttagccg cttcaccaat tccctgaacg ccttctttca ccatatcgcc
     9781 gaagtttttc agcccttctt ttgcttgttc accatattga ttcaagttat cttgccaatt
     9841 tccagactct ccattagaat ttcctgtgtt tccaacttga tctgcagcta ccacttgtcc
     9901 tcctgtggca taggcatctg ctacactaaa tcctgttttt gaagtatacg gaaggatgcc
     9961 ttctgccgct gatttgaaga ctttcccgac aacatttcca cttgtccctt ccaaatagtg
    10021 cagcttgctg gattcttcgt aacccatcca tgtcgaaatc actacgtctg gcgtgtaacc
    10081 gatcatccat tggtcatttg cttttgtggc atcgaaatta gtttctgtgg ttcctgtttt
    10141 ccctgctatc gtataacctg caggattcgc agcagccgct gttccatttg aaaatgtacc
    10201 aagaagcatg ctggtcattt catcggctgt ttcctttgat atgacctgtt ttggtttttt
    10261 gtttgtgtta tcgacgatca ccgctccagt cgaatcaacg attttcgtga taagatgcgg
    10321 cgtatacatc tgaccatcat tggcaaatgc actgtaagca cccgccatga tcaatggaga
    10381 tactcctttt tccagtccac caagggctaa gccataatac ttatcagatt ttgctaaagg
    10441 caggccgaat tcttctgctt tatcatatcc tttttgtaaa ccgatctcat gcaaaagcca
    10501 tacagtaggc aaattcaaac tttgtgcaac agcttgatac atcggtactt ctccttggta
    10561 tgtcccgtca aaattatgcg catcgtaata actttgcggt tcgtctttca agatactgga
    10621 aggcttgtat ccagcttcca gtgctggtgt atatacgctt aatggtttga tcgttgatcc
    10681 tggtgaacga cgcatctgtg tagcaaagct gaatcctcta aagacatgat ctcctcttcg
    10741 tcctactagt gcttgtacac cacctgtatt cggatctaaa gctactgaag cagattggac
    10801 catggcacca tctgaagcat ttgctgggaa taatgcactg ttttgataag tagcttctaa
    10861 ttgcttttga taatcctgat ctaaagctgt atagatttta tagcctttat tcatgacatc
    10921 ttcttcatca agaccatact cgttaactgc ttcatcgatc actgcatcaa aataataagg
    10981 ataacgataa ccagaatctg aattatcgta tgtgtcatta agatacgcgc tgatatctgt
    11041 ctggctttct gcttgttctt gttcttttgt gatttttccg ttttcttcca ttacacttag
    11101 tactgtgttt ttccgatcat tcgcattttt tgggtgatcg atcggattat aaataccagg
    11161 acctttcagc attcctgcca aagtagcagc ctcagacagc gtaacattcg ttgcatctac
    11221 tccaaaatac tttctggaag cgtcttggac accccatacc ccgttgccaa aataggcatt
    11281 gttaagatac atggtcaaga tttcttcttt gctgtacttc ttttcgatct cgattgccaa
    11341 aaataattcc ttcgcttttc gatcaaacgt ttggtctaat gtcagatacg catttttggc
    11401 aagctgctga gtgatcgtac ttcctccgcc ccctgatgtt ccgccagaca agaccattcg
    11461 gactgctgca cgggcaatcc ctttgatatc aaacccgtga tgctcgtaaa aattcctatc
    11521 ttctgtcgag attactgcat cttggatata gggagaaatc gcgtttaatt ctacatatgt
    11581 tcctttttgg gcgtacagtg tgccagcttc ttcgtttttc ttatcataaa tgactgtcga
    11641 ttggcttaat cctgatttta acgtttcgac atttgcttgt tttgctaaaa taaagaggta
    11701 gatacttgtc actaagacac agatcaaacc aatcagcaaa atcaatttgt tcacatgata
    11761 ttttttccag atgcgctttc gccatcgatg gaattgtccg agatatggtc tgatccaacg
    11821 ccaaaaactg atcaaagcag cttttatctt ttctaaaatg ctttgaaaat ccatttgctt
    11881 cttctcctgt ctatttgatt caatcgtctt attttaccat agtgaaagag tgttttcttc
    11941 ctgcttaagt tgtagttcaa tatgtttttt tacgttagtg caagtagagt aacataagaa
    12001 tgaacagaaa aatgtaagtt ttttcttaaa tatgttttaa aaatggtaca attagtctgc
    12061 gattgcactg aaaggagtac acttgtgaaa atcacgatca ctttacctat gaaccatcct
    12121 gaaacaacaa ttcgcgactt gcttgaaaag gaatggttag ttcctagaaa ggttcgacac
    12181 tttttgcgta tacgtaaaaa cgtttggatc aatcaacaac ctgctttgtt ccatcatgaa
    12241 gtaaagggca atgaccagat cacattgtta ttcgaagaaa cagattataa ttatcaagaa
    12301 gttcaattag gagatgcgaa gcacatccgt gttttatatg aagatgagca tttgattata
    12361 gtggataagc ctgcaggaat gaagactcat ccaaatgaac cgacagaaaa cagtacctta
    12421 ctcaatcatt tagctgctta tctcgacact aaacagcaac gaccttacgt tgtccaccgc
    12481 ttggacaaag aaacaagcgg tgccgtttta tttgcaaaaa atccatttgt cttgccgata
    12541 cttggaagaa tgttggaaca aaaaatgatc tatcggagat atcaagccat cgtatgggga
    12601 gaaattgcca aagatatgac catcaccaat aaaattggac gagatcgcca tgatcggaga
    12661 aaaagggtcg tagatgataa aaatggtcag agcgccttga cacatgtcac tgtcaacaaa
    12721 gtactttctg aacaaacaca agtctattgt gttttggaaa ccggacgtac acaccagatc
    12781 agagttcatt tagctcataa gggacatccg atcgttggag accctctcta ccaaaataga
    12841 acagctgaac gactgatgct ccatgcatta gaactgcaca tgatccatcc atttactcaa
    12901 gaaaaaatag tcggtaaagc tttacctgga ttatgggaat agaaataaaa aagaacagag
    12961 tcttatcagc agataatgat aagactctgt tctttttgtg taatttgtct gcttactttt
    13021 gatgttcgtg ttctgctaac agcaaactac cgataatccc actttcattc ggcagtcccc
    13081 aacgaacgat atattcactt acttgaggtg tttccatata accacccatc aattcaacaa
    13141 attgctgacg gattttttga aggagatgat cctgattcat taccccaccg cctaaaataa
    13201 tcttttcagg tgctaacgtc aacgtatagt tgatcagtgc ttgagcaata taataagcct
    13261 gaatagccca aacaggatga tcttgcggca gatcttgccc tttgatccct gtgcgtgcct
    13321 caatagacgg tcctgctgct aatccttcta aacaatcttt atgataagga caagtacctt
    13381 cataatggtc atctggatgg cgtttcaccc aaatatgtcc catttcagga tgtgcgatcc
    13441 ctgaaaaaat ttcttcattg atcacgacac ctgcaccgat tcctgttccc acggtaaggt
    13501 aaatacaact attttttcct tgtgctgctc cttgcttcaa ttccccatat gcagcagcat
    13561 tgacatccgt tgtccaagca gcaggaatat tgtagcgttc cttgattgtg ccaagaaaat
    13621 cgaattgtgc ccaccctttt ttgggagttg ccaaaacgta tccgtaatta tcttttgttt
    13681 tgtcgatacc aatcgggccg aatgagccaa tcccgattcc cttcaattca taacgatcaa
    13741 aaaattcaaa tacctgatcc aatgtttctt ctggtgttgt ggtagggata cttaattttt
    13801 ctaaaatttc tccaccatct gaaaccgcac acacaaattt tgttccgcct gcttctattc
    13861 cgccatacat tcgttttcct cctcgaatcc gattgtccga atctcataag gttggattat
    13921 tgggacaacc gcttgcttcg ttggttcttc aagcagattc aatatgacag tttttccatt
    13981 gtctttagtt atttccaatt tttctaaatg actagacata ttgaaacctc ttactactac
    14041 tttatcacta aacttgctgc gtttcaaagc tgtaattgca aacgtttcac tttttatcgt
    14101 taaatattgt tgttttgaaa tcaatgttcc ttcatgatgt ttgatttgtt gagtggaaaa
    14161 aggaatctgt gctgcgtatg catgcaaata agtagagaat ttttcttctg gtccatgtag
    14221 ttcaatgctg taattgaacc gatgctcacc tagacactgt gcttctggtg ttggaaaata
    14281 tccccaatcg cctagttctc ccacactgcg aagtagcgtc actgcgattt gtccgtcttc
    14341 tgttacttca tattcattca aaccaaagtt tccgactgtt acaccgtact cttcatcatg
    14401 aatattgaca aatgcttgct gatgttgcgg attcgtgggg ttttcccaac ttttagacac
    14461 atggtttggg cgcttcacca cttcaaaaat actatctgct tcgtgatgtt ccacatgtaa
    14521 ctttgtagga aacaagacac gtaagcgatg atctttcatc tgattgtcga gagtcgtttc
    14581 aaagtcgatt tttttactat cttttctgat tgtcatgatt gtttttattt gaaggattcg
    14641 tttttctttg gatcgttctg cttttcgata acgaaattcc acaaccatct gctgttcttt
    14701 ttctaaacgc tcgtctgcag aaacagggat ttccatttcc tgtagaatac ttatttttgc
    14761 tatttccggt gtatctacta atacttttac ttcagagttt tctttgttac tggataggat
    14821 tggctgatca cagaaaggtt gtttaaagat atactcattt cctatatcgc cgatatcttc
    14881 aaaaatatgc agattattca actttcgttt gtttttgcga tcttccattg taatggtacc
    14941 atttttttca atcgtcaaat gtagagggcc gttttcgatg attcgtccac tctgatgaat
    15001 cattgtttcc ttttcttctg ctttcgcttc cccttctaca agcgcgaatg tttcccaaga
    15061 aaatgctggc atgttttcta atggtaaagt cactttgatc attcttgcca tatatggcac
    15121 acggaaacgg tctttcggca aatcataacc aaattggaca gtttcttcag ataaaatagc
    15181 tggtatagtg gccccacttt ttgtttctac atgatactta cgtttaggca aattttctaa
    15241 ctcttgatat agttgttcag gtattccttc agcgaatctt tttctttcta attcgatggt
    15301 tatttctgct tcaccagttt tatccattcc tgcagtattt acaatgacaa atggaaagct
    15361 gtcttttggg aatccggtcg tatcgattgc tgcggtcagc tgttctaagg aatcttgtgc
    15421 aagatacttt ccaacttcat ctgctttttc aaaacgaggt atcatttccc gatggacaga
    15481 atcgactgaa caaccgcaaa tactgtcgtg aggatgattt tgcattaatg tcttccaagc
    15541 ataatctaat tgatcatggg gatagtttcc actaacttca taagccatcg tcgcaagtgg
    15601 ttctgtaata ttttctagct gtcgctggac cttggtattc cattgtttta agtaaacccg
    15661 agcagatgct gtattggcta aagtatacca gccatccgtt tcttgacttg tcaattctcc
    15721 ttctactgag cctaagtctt ctggcacatc tttttgaact gcttccagat agtcagtaaa
    15781 attagaatga ataaactcgt aatctggaaa cagttcattt gcgagatgga ttgcttttga
    15841 tatatctttt tgtaccggtt gatgatcaac accattcatc atcaataagt ggttcgtaga
    15901 ggcatattgt tctgcatccg ctaatttttg cttccaaaag gcaagagctg cttctttttc
    15961 tactggaatc tcattcccgt tgctatacca attggcaaat agcagaccaa agattgctgt
    16021 ttgatcaggt cctttccacc acatctctga gtattgggaa gaatagtttt ctgcttccaa
    16081 aacttgatta tcaaagccaa ttggttttac accacggcca aacgctgctg ctgaaatacc
    16141 agcttgtttc attaattgag gcgtctgtcc catatttcca aatgtatcgg gaaaatagcc
    16201 taacatgaca ggggttcccc atttacggct ttcttccatg ccaatcagca tatttctcac
    16261 gttcgattcc gagctgataa gaaaatcatc ttgcaagata taaaatggtc caattctcaa
    16321 ttttccttca ttgattgctt gttggattgc ctgtcttttt tctggcctta cttgaagata
    16381 gtcatctaaa ataatcgttt gcccatccaa atgaaaactg ttgaaggacg gatcaacttc
    16441 aaatagctct agtagatcgt ctatcaattc aatcagtcgc atgtgatgct gttcgtaagc
    16501 catgtaccat tcacgatccc agtggctatg agaaatgata tatactttct ttttcattta
    16561 cgatacctcc tacttttcta ctctaatatc aaagtaatcc atgactaatt cgcaaaacat
    16621 catatttgcc caagaaaacc attctctcgt ataattttca ggattgttca catcgaatcc
    16681 ttcgtgcata aggtgtgttc ctgcgtcagt tgctacaagt aaattcaata tccgctcttt
    16741 ttcagatttg tctgacgttg tcattccttc catagctaat gcaatcggcc aaacgtattt
    16801 ttctggcgta tgggaggatc caattccttt tgcgtacttt ccttcataga aatatggatt
    16861 ttctttactc aacaatgttc ttcttgtttg cagataatct tcctcttgtt cagaacaata
    16921 tcccaagtaa ggtgcggcga tcagattggg cacattactg tcatccatga ctgtggcgtt
    16981 tcccattcca tctacttcat atgcataaat cgtttgccct ttttgattaa ccgttcgtcc
    17041 gtgattctga atcccctctt ggatctcttt cttcaatttc tgtgtttgct gtacaatttc
    17101 tttatcatcg agtatcgttt gaaaaatttc ttccacataa cctaacacga ccaccgcaaa
    17161 catattggat ggtaccagat atccataacg gcaagtatca tcgctaggac ggaaaccaga
    17221 ccatgtcatt cctgtaaaag ctgtttttgc acctcgcccg tcattgatca gcgtatcttc
    17281 ttttcttgtc gtgtctcgta caaatgtata aggagagtgt tcatgatctt gctctgtttt
    17341 gaacacgtcc aagatttttt tgataccttc aataaaagat tgattgaatt gatcagtttt
    17401 tcctgtattt ttgtaaagaa gataggccaa ctgtattggg tagcataaag aatcaatttc
    17461 atattttctt tcccagatcc aatcgttcat ttttgtatga tctgtttggt ggccagcacc
    17521 gttagcttct tcgttgaagg cattcgcata tggatcgata ttgatataaa aaaattgctt
    17581 tttgactaac ccgctgatca tgtcagctaa ttcctcatct tcttttgcaa tcactaaata
    17641 aggacgaact tgtgcagtag agtctcttaa ccacatcgct ggaatgtctc ccgtcaataa
    17701 aaaggtggtt ccgtcatttt gctttttgac tgttgtcaac agagtattgg caaatgctgc
    17761 tgtaaaattc tttgcccagt ccgcatggat atccccacat ttttgtgtga tatcttccat
    17821 aaatttctgc accgattttg gtatatcttt ataagccact aataaaacct tctttctatt
    17881 tgatatatct ttaaagatta tacttcatac agccaattat gtctacgctt tcaaaaaata
    17941 tctttttatt tactttttca tccattatgg tattatttga atatgatata tcattaagag
    18001 gtgtttatat gaatcaacca ttgtaccaga caatatttaa tgatttgcaa acggctattt
    18061 taactggtga attacctgta gacagccaac taccaactga aaaagaactt tcacgtacct
    18121 ataaagtaag cagaatcact tccaaacgtg cattgactga attagagcaa ttaggactta
    18181 tttatcgcgt tcgcgggaaa ggcagtttcg ttaaatccgc ttctccggaa cagattcttg
    18241 cttctcctac aaaaacacga cggattctgt ttcttcttcc atatgtcgct gatttgtctg
    18301 ttggtgattt tagcaaggga ttgaaccctg tgatgcaaga taaacagatt gatgtcatga
    18361 tgacgacgct tgactttcta caaaataaaa cagcaaaaga gctaatcaat gaatttgacg
    18421 gattgattta ttatgctctt gatacagaac aacatcttga cttacttttt gagctttcat
    18481 tgaaagaatt ccctgtcgtc atattagata aaaaaatcta tgagttacct tttcctaccg
    18541 ttctttccga taatttccaa ggcggtgcgc ttgctgcctc ttatttgatc gaacagggac
    18601 attcaaaaat cgcttatttg tttggtacgc agactcatcc acaatccgtt cgacagcgtt
    18661 accttggtta ccttgaagca ttggacaaag ctggtttgac tttccacact tcactggatg
    18721 acaaaagcgc gacattagat gaattgcttt cttatatcac taagtatgaa gtaactgctt
    18781 ttgtttgtga aaacgatgtt gtcgctatcc agaccatgaa tctcttgaga cagcatggtt
    18841 atcatatccc tgaagatttt tcaattatcg gatttgatga tatccaagca gcagctcttg
    18901 ttgacccgcc actgacaacc atcgcacaag attttgaaca gttaggtaag cttgcgggag
    18961 atgctttggt cacttggtta gagacaaaag atcgaccatc cgatagtaaa tttccagttt
    19021 cattcatcaa aagacaatca acaaaggaga aataaaatga atatcggtca aatagatact
    19081 agacatgcaa cagcgaatca gccaaatttc tctaatggga actgccagcc ttatactggc
    19141 gttccttttg ggatgaatta ttttgcaccg caaactacgg atcaaaacgg cagctggtgg
    19201 tttcatcctg atgaccgcgt ttttcaaggc taccgtctta cccatcaacc aagtccttgg
    19261 atgggtgatt tcagtcatat gctgtttacg ccgatcaatg gaaaactaca ggagaacacc
    19321 cttttccatg ctcaaagttc ttaccgccca gaagaaagta tattttgccc gactcatctg
    19381 tcgatctgtc aattacgtta tggcatctgt tctacattga tacctagcat gtatggcgga
    19441 atcttatcta ttgattacag caaaaatgac agcggtttac ttttatcctt tccgggacgc
    19501 catcagctct ttgttataga tccttacaca gtggaaggaa aaatcacaca ttttgctggt
    19561 tcagaagatc ctgatttttc tttttatttt atattgagat ttgaatatcc tttaacaatt
    19621 gatgagttag ctgtttccaa caaaaatagc gactcgatcc cgttgtattt tgaacagaca
    19681 aaaaaacaaa ctatccgatt tgggacatct tttatcagct cggaacaagc agcttataac
    19741 ctggaactag aacaaaacca aagcaaagag acttaccttg ctcacagtca agaacaatgg
    19801 cagtcctatt ttgatcgtat acagctcaca caccatgatc aacaacaatt gcggacgttt
    19861 tatcataatt tatatcgggt gtttttattt ccacaaacat tttatgaaat tgatcctagt
    19921 gggcaaaaaa tccactatga tacgactagt cgatctataa aacctggtgt tctctataca
    19981 aataatgggt tttgggatac gtataaaacg gtttatcctc tttattcttt gattgctgta
    20041 gaaaaatacg aagaaatgct ggaaggtttt ttgaacagct atagagaaag tggctatttg
    20101 ccaaaatggc tgtctcctga tgaacgtgga cttatgcctg ggacattgat cgatgcggtc
    20161 attgcagatg ctgctgtaaa agatatccga cgagacttga tgcctgaatt tctaacggca
    20221 atggaaaaag gtgctacggt tcaaagcaaa catccaaatt atggacgcca aggaacagaa
    20281 gattacttga catatggcta tgttcctaat cactaccacg aatcagtcaa tcacacatta
    20341 gactatgctt atagcgattt ttgtattagc caagttgcca aggtactaga agaagaggaa
    20401 atgacgaaga aatatcagca gcaatcattg aactatcaaa atatctttga ctatgagtca
    20461 ggatttatgc gttcaaaaga taaagatggc aagttcagag aaccattttc agctaatcgt
    20521 tggggacaag attatgcaga aggaagtgct tggcaaagca gctttgctgt ttatcacgat
    20581 ttttcaggac tgatcggaaa atatggtggc agtgagagat ataaagaaaa actcattgag
    20641 ctgtgcaatc agcccccaac gtttgaccca ggaggatatg gttttgagat ccacgaaatg
    20701 agtgagatgg ccgcgataga atttggacaa ttagctattt caaaccagcc aagttttcac
    20761 tatccttact tgttcagtta tataggacaa cctgaaatgg cacagccact gcttaaacag
    20821 ttgatgactc agaccttcga cgattctcca atgggttacc ctggagatga ggacaatgga
    20881 agtatggccg cttggtatat cttcaatagt ctgggatttt atcctgtcac gcctggaacc
    20941 ggtgaatatg ttatcggtat gccattaatt gacaaggctg ttttgctttt gtcaaatgga
    21001 aaggaattga cgattgaagc aacgcctaat cggccgcagc aacaatttat ccataaaatc
    21061 aaacggatgg acaaagaata tacgaaatta tattttaccc atcaagattt gctgaaaggc
    21121 ggcacgatct cttaccaatt agggatcatc cctcacccta agaaatatca agcaaaagat
    21181 ttgccttttt ccttatcaaa ctataattaa caagaggttg tgaaaaagct gtcctgcatc
    21241 aagtaataag aacagccaag caaaaataga tcctcatatt tttcgtttgg ttggtcttat
    21301 taccgcagat gcaagctttt gaacaccgtt tattgagagg ttgtgaaatc agcgttctga
    21361 cctgagtatt agagagacaa atggcaaaat agttcctcat attttgccat tttttgagct
    21421 aataccgagg gtcagctgat tgaacaccgt ttagtggaga ggtcgtgaaa aagctgttta
    21481 gtttcgagta ttaagaaaaa ttttgtgaaa atggctttcc acatttttgc ataaatttgg
    21541 cttaataccg agaaactggc ttttgaactc cgtttatcga gaggtcatga aaaagctgcc
    21601 ttgcctcaag taataagaac agcccaaaat tagatttcta gacaattttt tataagttat
    21661 ttcattacat tggctttttc ttttatgatt ctccactgtt ttctcggtac tctttagggg
    21721 atctgccgac gattttcttg aataatttag agaaatacgt ggtgttgttg tagccaatct
    21781 cacttgctat ctcattgatg ttttgattac tgtgcaaaag cagttgtttc gctttttcca
    21841 tacgtaaatg gttcaaataa gctgaaaaac tttttttcgt ttcttttttg aacagctgtc
    21901 ctagatacat gacatttaaa tgcaggtttt ctgacacttc tttgagtgta agtggttctt
    21961 gataatgctg gtgcaggatt acgattactt tttccacatt gtcactgtac tttttctgtc
    22021 tctgatgctt tttgatgaat gacagaagta gctgctgaag ttcttgcacc gtttttgcct
    22081 gatggatttt ttcgattcct tgtaaatatt cctcatcttc cagatcaatc aattcccgtt
    22141 gaatctccat aaacagtaaa aaactggaat atcgtatatc ttccggcatc atggctgcta
    22201 actgaaactg ttcaaaaaaa tcagtgatca ttttttgagc gacttccaat tgtttatttt
    22261 gcagcacgcg tctgaaactt gaaaaatcga tggtttgatc ttttgtttct gcttgtactg
    22321 cgtaaaaaac atgctcgtct ttatctccat aaaattgatg caactgtaga ttgtctttcg
    22381 cttgttggaa tgtttcaggg attttgtcca aagaatctgt ctcttccccg atactgatca
    22441 gccattcctg atcaggaaag ttttcttgaa caaaacaaca gaagccatca acttcatctg
    22501 cctccaacaa caacgtgatc aatacacggt ctccgtaatt tcgttgatag taaaagattt
    22561 gttgccgttc ttgcaaccat ctattgataa gagtatcatg agttcttgcc agttgaatca
    22621 atactatcct atgctgcttc tcacttattt gtcctattat ttcttcttca cttatttcat
    22681 ccaattcttc attcaaccat tgactgaata acagttcttg atagatttct tgttgatttt
    22741 tttgttcatt ttgctgatct aaccgtgttt tgattttctt gaggctttca accaattcga
    22801 acttattcac gggtttcatc aaataattca cggcgcctaa ttgcatacct cctttcaaat
    22861 aatcgaattc ttggtatcct gataaaatca taaattcaaa attatgacgt tctttttgag
    22921 ccgcttcaat aaattccagt ccatttattt ctggcattgt cacatccgtt actactaaat
    22981 caatctgtcg ctgttcaagt acagctagtc cttctttggc attctctgct gttgctacta
    23041 tttgaaagcc taaactttgc caatcaatta tcttttttaa cccattcaaa atcatgtatt
    23101 catcatctac caataacact tgatacacgc tcatccctcc ttgaatgtaa tagaaacggt
    23161 cagtccacct gtctcattat tctcaagcga catataataa ctgctgccga agctggcacg
    23221 taaacgttca ttcacgtttt gcagaccgat agaaccgtga agttccactt taggatgaga
    23281 cagtttttct tcaatctgtt ttagccgttt ttcagtcatc ccttttccat tatctttgat
    23341 gatgatcctt acccgtttgc cttcctgcaa gactttgaca cttagcgcat tatcgaatcg
    23401 agtaaaatca ataccgtgtt taaaataatt ttcaactaat ggctggatca caaatttagg
    23461 tacttcgatc ttttccaaat caggatcgat cataaaatga taggctactc gattaggata
    23521 acgcatttga tacaaataga catatttttc gcaaaagtcc agttcttctt ttaaagtgat
    23581 cgttttctct tgattcgtat tgtttcgaag caaagcggaa aacgcataca caacatctgc
    23641 taattcttca cttccttcgc tgattgcata cattcggata tattctaatg tattatataa
    23701 aaagtgtgga ttgatctgtg cttgaagcgc ccgcatatgt gcgtcttgct gtttgatttc
    23761 taatttgtag atgtcttcta caaactgatt gatgctatcc aacatttcgt tgataccgat
    23821 tgaaagttcc ttcagttcat attccgtgtt tgttatgtcg atccgcgtct ctaaattccc
    23881 tgctgctgtt tgatccatgg cctgcatgac gatagatact tgctgtgaat atcgtttgaa
    23941 tgttttataa agaaagataa gcagtatatt gtctaaaata atgataccaa tgatcagcgg
    24001 ggcaagattt tttcccgttt cgatttttac acttttttta tctagtacag ctaatatacg
    24061 ataatcatca gacagttctt ggtgttgaac aaaattgttt ttttccagat cgtcaatcgg
    24121 caattgtgaa tttttcttca gactctctgt gatgatcatt tcttgtcttt caaacgtttc
    24181 tttagctatt ttattatcat ggaatgtgta tagtcgattt gtcgttccag aaatcatgta
    24241 taaagagagt ccattgaatg acgttagatt ttctaaaatc atatctagtt ctttcttttg
    24301 aaaaccaata aaaatacttc ctgactcagc tcctccttgt gtgataggca acttgatata
    24361 aaaggcgtct agtaattttg gtgtcccctt ttttatttcc ccacctttgt tttctctaga
    24421 agattcataa tactccggta gttgattcaa cacgatatat atcgcactta gttcttcata
    24481 atttgcgtag agatctttga tttgatttgg gaaagaaact aattcatcgg tactgttttg
    24541 ctgttcgtat acatacatca gatactgatc tatgggtttt gttaagtagg tcgtcacatt
    24601 ttcgatcttt tcggaattcc ctacaagctg gtttttgatg atcctactca aggttgtttt
    24661 atcattcagc attctgcttg tgctttgaac ggcatcactt gtcatgtttt ctgcctgttt
    24721 ttgattttgt tcccaagtat ggatactaaa aataacggta gcagtcatcg ttacaccgat
    24781 catgataacc gcgtatttcc gcaacagacg atttagtaag gttccttttg aaaatagaat
    24841 ccgaaactta ttcataactt tccaactgtt tatctaattc ttcacgccaa tgtgttgtca
    24901 tcgtaccatt ccaaaccgtc aaaagaccaa tgacaccaaa caggatcaat cctttgaatt
    24961 gccaagtcac gcctaaaatt ataccgctac caataattat ttttagaaaa gacgaaaagc
    25021 tggaaaaaac cgaaataaaa cttagcttta gcagattagg aacagagatc gtatattcac
    25081 tatctaaaat catactgtat tgataagtag caaataggca gagcaaggca aaaagtaaaa
    25141 taaaatcaat agctaaaaaa atgagccctt tgatttgaac agataaatat aaattgtaac
    25201 tcaataaagc agtaccagat aaaaataaac taaatagcag attgcctctt aaaaaatttc
    25261 ttttatatat attccagcct ctttttattg tgatttcttt atactcgaat ccgtacaaat
    25321 aaaatagttc attgactgtt ttccatgccg cacctaatcc aaagatgatc ccgccggcaa
    25381 atgtatatat ccagaaatac aaggttagtt ttataatgac ccagatccgg ataaataact
    25441 cttccaaaac cttccctaac atatcttctc ctccaaacat taagtttata ctttctcatt
    25501 atacaaaaaa aggttgggca atcagcacct tgacctgatt attagagaaa caaacggaaa
    25561 aatagttcct catattttgt cgttttgtga gcgaataccg agggttagct gattgaacac
    25621 cgtttaccaa gagtttgtga aatagccgct tagtttcgag tagtaagaaa aattttgtga
    25681 atatggcttt ccacattttt acatatattt agcttaatac cgagaaactg gcttttgaac
    25741 accgtttatt cggaacaaga ggttgtgaca cgtcttttgt cacaacccct ttgtctttat
    25801 tcattattat tcttgtcgga actcatcata ttgtttttgc atttctttca ataccttatc
    25861 atagccagcc ttgtccaaag cgtctttcaa tttcggtaat gtttcatctg gatcaacagt
    25921 ccctgtattc aatccatcta aatattgatt catgacatta gaaatattac ttaattctgt
    25981 tttgacactg tcagtgttga agctgaatcc taaaatcggt gaagtttctg catcagcaat
    26041 cgattgatca cgtttggcaa tcatgtcatc cgtaatagac tcttgtgtat atagaatctt
    26101 gttgtttcct gtattccaag cagacatatg tgtatttggc tgataaccat ctagcaattt
    26161 gattttatca tctgaaccat cgactttttc ccatgcttct ccctcgatcc cccaaacaag
    26221 cccattcaat aattctggat cactgttaag cagccctaga agttctactg atttttcttt
    26281 attttttgaa gtatttgata cgacaaaatt tgccatttgt gcttgagctg tagattttag
    26341 tggaacagta attgcttttg agacaagttc ttgttttgct gcatttgtca aaattgtatc
    26401 gccataatca tacggtcctt gtgtttctcc acgcattagc caagtatttc cttctagtgg
    26461 ataatcctta ttgctcgttg cagcgtcact tggaatgagt ccttgtttgt accagctatg
    26521 cattgtacgt aacagtccga tatacgtttc attatcgtat tggttgatga ttttcgaatc
    26581 atctccatcc agatcaatag caaatggtaa cgtattccct aaaggataat caaagttccc
    26641 ttggactttg aagccttgtc caatggcaaa agctgccgtg tttggttctt tttcatggaa
    26701 ttccttcaag actttttctg catcagcgta agattggatt ccatcaattg aaagatcgta
    26761 tttatctagt aaagctttgt taaaagtaag tacttgttga gcaaagacat ttccattgac
    26821 tgggaaagca tataattttc cattgaccag atttccttta atatatgctt catctaaatc
    26881 attataagct ttttcagcgt acttaggcgc taattcagtc aaatcagcaa aggctccttt
    26941 ttgggcattg gaaacatagt tgttcgcaaa ggcgatatca taattttccc cggaagaaat
    27001 gatgacgttc attttctttt cgtaatcgcc ccagccaatg taattgatat tgacttttac
    27061 accgatctct ttttcgattc gtttgtttgc tacttccatc aattgatcga aattatctgg
    27121 tttatctccg atttgataca tctgcaaagt gtccgaattg ctagcttctg cctggtcttt
    27181 agaaccagaa cctccgcatg ctgctaatgt tcccaacaac aacgctgaaa cacctgtaaa
    27241 ggctagtttc ttccataact tcatttttct tcctcctaat tttatgattt atatttttgt
    27301 ttctttattc ttttactccg ccgattgtta gacctttgac aaaatgcttc tggaaaaatg
    27361 gatagctgat cgcaataggc aacgtcgaga tgactacgat cgccattctg gcagattctc
    27421 ctggaactga ggctaatccg cctgtcaatt ggcttcctgc tcctgaattt tgtgtcagat
    27481 attgaatatt actttggatt ttcatcaaaa gatactgtag tggaactagc ttatcatctt
    27541 ggatgtatag taaagcattg aaccagtcat tccagtaacc caatgcggca aataaactga
    27601 ttgtcgcaat acctggaaca gctaacggta aaacgatctg aacaaatatt cgcagttcgc
    27661 tagctccatc gatccgtgcc gattcaataa tcgaatcaga gaccgaacgt ttaaagaatg
    27721 tgcgcatcac aataatattg aagggactga cagccattgg caagatcaat gcccaaatcg
    27781 tatcttttaa ctgaagaaga tttgtcataa caagataatt ggccaccatc cctggtgaga
    27841 acaacatcgt aatcaaacaa aaaacggtaa aaaaacgacg gaatggaaag tttgaccgtg
    27901 aaatcgcata agcatataaa gaagtagccg tgctattgat cagtgtacct aaaacagtaa
    27961 caagtacagt gacgaaaagc gcttggaaaa ttttatcctg catctggtcg aacaagtacg
    28021 tataaccaaa tatactgaac ttttctggcc aaaaactgta cccgtgagtc gacaatatgg
    28081 actcatcggt aaaagagatg gcgataacaa atataaatgg caaaatacag gaaatagcaa
    28141 agagtgcaat cattatatta gagaaaaaat tagccgtctt attgaaagag cggacttgga
    28201 ctctctccac tttctttttt ttcatttttt tcctccttag aataatgcag aatcgctatc
    28261 aaaacgtcgg gcgatcgtat ttgtgatcat caacaagaca aagccgacaa ctgattggta
    28321 aagtcctgca gcagccgtca taccaaaatc tcctgtggat gtcaggccat tgtagatata
    28381 cgtatccaaa acttgagtca catcatataa tgcaccggaa ttcctaggaa tgttataaaa
    28441 caggccaaaa tcagctcgga aaatgtttcc tacggctaaa atcgttaaaa tcgtcattag
    28501 tggaataagt tgtggtatcg tcacatgttt gatctgctgc catttgctag ctccatcaac
    28561 catagcagct tcataataag tcggatcgat tcccataaca gatgcaaagt aaataatgct
    28621 gttataacca atccctttcc atgtaccaat gaacaccaag ataaacggcc aatatttcgg
    28681 ttcattatac caattgacag cttctcctcc atgtccagtg atccactgat tgaatattcc
    28741 tttgtcagga cttaaaaaag cgtaaacaaa gtaactgata atgacccaag atagaaaata
    28801 aggtagtaag gacatcgttt gataaacttt aacgagtcgt ttatttctca actcgctcat
    28861 aatgattgca aatacgactg agatcagcag attcaaaagg ataaatgcta cattatataa
    28921 aattgtattt ttagtgatga gaaatgcttg atccgaagaa aataaaaatt tgaaattttc
    28981 ccaacccacc caaggacttt ccctcaagct agccaagaac ccatctgctg aaatatgaaa
    29041 atttttgaat gctacgacat tcgccattac aggtatgtaa aagaaaaaaa tcatccaaag
    29101 catacccggt acagccatca ggacaagtgc tttataacgc catagattca taaaaaaatg
    29161 attgaacccc tttcgttttt tcatctaaaa acccctttcg attcattcgc aacgtaatta
    29221 tagcgctttc aataatgaga ataaataaac aaagtatagg gaaaatgaaa caaagtatag
    29281 gagaggttgt gaaatcagcg ttttggcctg agtattagag aaaaaaacgg gaaaatagtt
    29341 cctcatattt tgccgttttt tgagctaata ccgagggtca gctgattgaa caccgtttat
    29401 cagaggttgt gaattcagcg ttttggcctg agtattaggg agatcgtgtc cttactatct
    29461 aactctgagt cacaaggaac aatggggact gtaacaaaag tcttgtcaca gtcccttatt
    29521 ccgaacaaac ggtgttccag aagtagcttc tacggaaata agccgaaatt tcacaaaaaa
    29581 gccatccgaa agtcagacgg acaagtctgc ttttcagatg gcgctacaac tactctgctt
    29641 attcttttag ctatccattc aattattttt ctctcacttg ttctagctct tctttggtca
    29701 tgggtcgata ttgcccttta ttcaaatctt taggcagcca aaattttccc atacggattc
    29761 tttttaaata taagacttct tttcctactg ctttgaccat tcgcttaact tggtgaaact
    29821 ttccttcatg caaaatcaga cggataattg aagtttctgt ctcttgatct acttgatcaa
    29881 tttggagttt tgccggcaat gtctgctcat cgccatcaat aatcagtcct tctgcaaata
    29941 aacggatatc ttcttctgtc attataccgc taacctcagc tagatattct ttctccacat
    30001 gttttttagg tgatagtaac tgatgggcaa acaccccgtc attcgttaaa atcaagaggc
    30061 cctctgtgtc tttatctaat cggccgactg gaaaaagatc ttcccgataa tcctggtctt
    30121 ccaataaatc gattacggtt tgatcatgat tatcctgagt agcagaaatg actcctgcag
    30181 gtttatgcaa aatgtagtaa aaatcttttt gatacgtaat gatttctcct aaataagtaa
    30241 ccgcatcttc gaattcattt acttgcgctt tatcgctttt caccacttgt ccattgactt
    30301 cgatcgttcc ttttttgatt aatcctttga cttctttgcg gctgccaagt cccatatctg
    30361 ctaaaaactt atctaaacgc atgcgtttcc cccatttcaa aagagcatca atctagctct
    30421 tctgattcac ttattttatt tttaatttat gtcgtaaacg cgctattcct ggtcctagta
    30481 gtctttctgc caagcggatt ttcaaagcta aataaccata cactaaagcc cctatacctg
    30541 ctactacaag aatcaacaat agagactgga atttgctatc ttcgtttaaa aaagcaccaa
    30601 aaatcatctt tgtgatccac gaagccaaaa gcataatcaa ggtaagaata aagatcagca
    30661 atgtcctgcg ccagacaaaa cttggtttaa agcgggctac ttcatggatt ctctttaaga
    30721 tcaaggtaca agacacagca aatccgatca ttgtcgccaa taaagggccg tatacttcaa
    30781 ataaacgaat ggctgggtat tgaagaagga gtttcactag aaacccaata accaaataat
    30841 taattgccgc cttgttttca aacattcctt gcagcatgtt ggaaactagc atatatagtc
    30901 ctaggaagag tcctacaaaa cttgcttgga tcagtacatt acttcctaga gaatccggtg
    30961 tataaaataa tgtatttagc ggataggcaa gtaacataac accaaatgtc gccggaaaca
    31021 taaaaaatga aaacaactgt aaatttccgc ttgtcaattt tgccagacct actcgatctt
    31081 tcaacgtaac agcttctgta atcaacggta acccagtaga cgcgatcgat gttgccaatg
    31141 caataactac catcgtcaac ttatctggat tggcactaaa aatcgaaaaa agatccagta
    31201 actgtgcatt ggaatactcg gtggatgcag acatgatctt cataaaagtg aactgatcga
    31261 ctaatttgaa gatcgttacc cctgaaccca caataataaa cggaatcgct tctttaacgg
    31321 tatctaacaa caattccttc gttgcaatat ttattttgtt ttcgctgtag tgaataaacg
    31381 ccgatgtata cgcttgatgt ttcttcaaaa aatacaaaag gacaagaata cttgctagca
    31441 taccgataaa tgccgcgaaa gttgattgcg tgaccgccgt cacatagtca ccatccatga
    31501 ctttcatgat gatgaatgta gccaatagca tataaaaaat acgtgccacc tgttctacaa
    31561 tctgcgacag cgcataaggc atcatttctt gattgccttg caaataccct cgtatcacac
    31621 tcatacaagg aaaaaccaat acagcgatac tcaaagaacg catcgttgga atcagttctt
    31681 ctccgccacc agaagcatga gctagccaag gagaggcgat gtacatgaac aaagcaaaca
    31741 acaggcctaa cccgcccatc atctgtaatg cacggataaa taatcgtcgt gaagtgccat
    31801 attcattcaa tgaattatag cgtgcagttt gttttgcaat cgcagcaggt attcctgctg
    31861 tagagatcag tagaaacaat gcatatatat tgtatcccat attgaataat ccattggcag
    31921 ccttagcatt ttcgcccatc caagcatacc agggaatgat atagacagcg cctagcaatc
    31981 ttgaaatgat attactggcg gtcatccaag tagagcctcg tgccattttt tcttgcggtg
    32041 ttaattgatc taatgatggt tttctttgat tatccatcgg tcagattcca actttctaca
    32101 aaacattact atcattttaa ttggtaaaac agaaactggc aatcgatttt atataacttt
    32161 tataagaaca attgtttttt tgaattcggt ttcgtttttt tggtaaagta aagaaatata
    32221 cagcctttta tggctaacat ttataggaga tatcaatgac acaagaaata aaaaaactac
    32281 ccgttatatt tttaggtctt ttctttatca cagttggatt gaattggttc ttgctccctc
    32341 acgatatagc ttccgccgga gtgggcgcca tcggacattt agttgaaacg gccttttcta
    32401 tccaacgctc agttactgtt tggagcatca atcttatgat gctgcttcta gccgctgtcc
    32461 tattaggaaa atcagtattt tttaagactg tagcgggaag tctgcttttt cctgttgttt
    32521 tagagattgt tccaaaagtg gaaatgatct cctcacattt cttttcactg atttcaggaa
    32581 gtcttttgtt cagtcttggg gtttacacat tgtacacagt cggggcctca aatggcggta
    32641 taacgatacc gccgattatt ttccacaaat tttttcggct tccaatgccg caaggacttt
    32701 tacttaccaa tagtcttatc gtctttttga attattatgt ttttggattg ttggagacac
    32761 tattcgttct cctatctatt acagtcagtt cattgtttat gaaaatactc atccgcttgt
    32821 cccctgtgtc aagaaaattt gaataaagct ggattttgtc atagcggaaa caaacgatat
    32881 attaaaaaga gtgtgctata ataaaccaag aaattgaata aagcgaggga tcaatcaatg
    32941 agcttctctt tagaagacat ccgtcattta ctattaaaag aaaatctatt aaaagaattt
    33001 gtttcaacaa gaggctggca tcttgatgta ccagaaaaag ctgaatttct tcagttaagt
    33061 tatgattcaa gaaaagctga tgcttctact ttgtttttct gtaaaggaat gaatttcaaa
    33121 gaagaatatt tggattcagc aatcgaacag ggtattcaat attatgtatc agaacaacca
    33181 tacgaaaatt cagcagtagg aatcatcgta acggatatcc gtaaagcaat ggcgcttctt
    33241 gctatggctt tttacgacta tccgcaaaat aaattgaaag tgatcggatt cactggaaca
    33301 aaaggtaaaa caactgctgc gtactttaca aaagcaatct tggatcacac aacaaataaa
    33361 aaaacagcat tgctttctac tatgaataca acgttagatg gcaaaacata tttcaaatcc
    33421 catttgacaa cgcctgagtc attagattta tatcgtatga tgtcagaagc agttgaaaat
    33481 ggtatgactc atctagtgat ggaagtttct tcacaagctt acaaaacaca acgcgtatac
    33541 ggcttgactt tagatgtcgg aatcttcttg aatatctcgc ctgaccatat cagtccgatc
    33601 gaacatccga catttgatga ctatttctat tgcaagcgac aattgatctt gaattcaaaa
    33661 acagttgttt taaatcatga aagtgactat tttgatttac ttagggaaac agccgatcta
    33721 ttccagattc caacgatcac ttatggtcgt tcggaagaaa gcaattatca agtgatccgt
    33781 tcagataaag gtactcacgg ttttacttta tcttctcatg aagatcgctt agctatctgc
    33841 gatactgctt atgatatttt gctggctgga ggattcaacc aagaaaatgc agccagtgcg
    33901 atcattgccg cagcactaac aggtgcctcc agtcaagatg cacaagacgg attaaaagaa
    33961 gctcgtgtac caggccgaat ggatcaattg atccaatcca atggagcgca tgtctatgtg
    34021 gactatgcac ataattatct aagcttgaaa acattgctga aatttgccaa aaatgaacat
    34081 ccagatggcc gtgtaattgt tgtattaggc agcccaggaa acaaagcaat ctcccgtcga
    34141 catgattttg gaaaagtgct atcagaaaca gctgacatcg cctttttgac ggcagatgac
    34201 ccagcatttg aagatccgaa aaaaatcgcg gaagaaatca atgaagcaat tactaatcca
    34261 gatttgatcg ttcattatga aatggatcgt cctgaagcga ttcgacgcgc tttagcagaa
    34321 agcgctccgc aagattcagt cgttattgcg ggaaaaggcg tggatcccta tcaaaaaatc
    34381 aatggtgtag atgaacctta tgaaggggat tatgcaatcg tgaagcggtt gattgaagaa
    34441 taatggtatt tatatactag tggtttccca tttctaagaa aaaaagggac tgtgattttt
    34501 tgtcacagtt ccttttttct tagaaatcat tttaactttt cactagctaa ttctgctgcc
    34561 ttttctcttt cttcatttaa taatttatta tcatagaatt tgataaatgg gaaccagata
    34621 atgaatgcga tgattgcaca aatgactgct aacacagcag ctttccaatc accaccacta
    34681 ccaacaaatg ctccgatccc gactggtgaa ggccacggca tctgtgcaag catcggtttg
    34741 acgatttcca gccgaatggc aaaataagcg agtgtagcag aagccatcgg agctaagaaa
    34801 aacggaaccg ctaaataggg attgtagacg attggcatcc cgaaaatgat tggttcattg
    34861 atattgaata atcctggtac aagtgaggct tttcctaaca ctttcagctg atcagatttc
    34921 gcgaggaatg caataaagat gatcaatcct aaagtcgcac ctgatccacc aacggttaca
    34981 taagaattgt tgaattctcc tgccaacgga taatttgcgc cttgtgcgtt agctgccata
    35041 ttagctaata cgatcggggt caagaaagaa gtgatgatat tcgctccatg gatccctacg
    35101 atccacaatg catggatcaa gaaataaatc accatgacac caagccatgt attcgtcaac
    35161 tgcgtaacaa acccaaatgg gatagcaatc attttgaaga tatccgttcc tagtgcaact
    35221 aataatccat tgatgatcat caccacaaac gcaatgacaa atgttgggat cagagcagtg
    35281 aaagaacgag aaactcctgg cggaacaaca tctggcattt ttatgaccca atttctcttg
    35341 acacacatac aatacaattc gaccgcaata atcgacataa taatagctgt aaagattcct
    35401 gaagttccta aacgttcgac aaaagagccc atcctaatac catttactac agtttctgtc
    35461 tcattgatag aagcaatcaa gctgatttta ccatcagtca tgatcaattc agggatacac
    35521 ataaagaatg caaaaacaga tagtaatgcc cccgtgagcg gattaacatt taatttttgt
    35581 tcatctcgtt caattgacgt taattcataa gcaaacacaa tggcaaagta aatagataaa
    35641 atccccatcg tcattgtatt gacgatcatg tacaagtcac taactttgaa aaaagttgca
    35701 ttaaaaaatc cttctaaaaa tgggaacggt aatggcaaaa tatttaaaac caaaaacatc
    35761 gagcctacaa tcgtgaaagg aatagaagcc atccccgctg ccatgacagc ccgaacgatt
    35821 ttaagttgcg caattttccc cataggtccc atcaggtatt tctgcaaaaa atcaaacatt
    35881 agttattctc ctttcctaaa tataacgctt tttcatattg tttaatacgg atatactgtt
    35941 ggtcttggtt gagtgaaatt ttctttaaac gatactgtat aaatgcactg gcaaaaatac
    36001 cgcttacaac aagaatgaaa acaaataact gtcctccatt gttatttgcc ataaaaggat
    36061 acaagaatgt aaatcctgag gtaaacaaca ctggaatcac taataaatta aagacactct
    36121 ggaacaaaaa ataccacttt gtccatggaa cgttcgtttg gtgttttcga taaagcttta
    36181 cttgttctcc tactgcaggc aaaatagcta aaatcagaat catcggtaaa ctacttccta
    36241 aacttttagc tgatagaagc agaatcatcc attgcatgtt catgaaaaag aaaaaggcag
    36301 tcaggtaacg tacgattaga taacgattga aatacatgtt tttcaaacta agttgctttt
    36361 tttctaattc gattttcctt tttactgtat cctgcacaat gatccttcct ttctatttat
    36421 ttttttgata caaataattc atttcctgtg caacttccaa taatgtcatc gtcgtcatca
    36481 gatgatcttg tgcatggacc atgataattt ccatttctat ttttgtcccg ctggcatatt
    36541 ctttcagaag ttgagtttgt gactgatgcg catgaagaat ctcttcattt gcttgtcgca
    36601 acttctcttc tacttcttca aacatctctt tacgcatcga gcgaaatgct tcatgtatca
    36661 gtgtccgtgc atttccgcta tggaaaatga tatcaaaaga agtcacttgt agtgtttcac
    36721 tgtttatttt ttcagccatc tgttttcctc cttattcgat cagtttgata aatttactgc
    36781 tgaaattctc aaaaataggt tcagctaaga tttcctgctg tacttttagc tggtcaacta
    36841 acctaacgat tgctttagtc acgaccgtaa ttccttcatt ttcgacataa gaaggcgaaa
    36901 ctaaaaaaac aaaatgaatt tcttgatatt gatcccactg catcccagaa ggaatcaatg
    36961 caacggctac ctttgtttcg attccaactg gaacagcggg atgtgggact gccactgtat
    37021 cactgaatat gatttgtccc atattttcac gttgctggat ctgctttcgc atttctctta
    37081 tatatccttc ttcttcattt atgctcagct tttgcagcaa atcatgaaga atttcttcct
    37141 ttttcggttc gtacgtataa gtcttgaaaa atttttcaga caactgttcg gtgtaaatct
    37201 gctttttttg ctgtagagaa aatgctggta tcggtctatc tgaggaatga tagggtatag
    37261 attctccaac tacctttcgt attttacgaa catcatcttc attcaaaaaa atactgacat
    37321 gtaaaactgg aattttgaaa aacattgtcg aaagatcaat agaagaaata atcaggtcga
    37381 ttccttttaa agttgtttca ttgatctcat aatagccttt tacacttacc actgtaatat
    37441 ttttaccgaa ttcactgact acgcgattct ttaatagttg ggcactgccg taacccgttg
    37501 cacagataat caatgcatgt agtttatgcg cagctctttc tttttccaat gccgccatga
    37561 gatgcaaagc caaataggcc cattcatctt cattgatggt atagtttttc aaagccggca
    37621 tgttgctgaa atgccttttt actgcgtcaa agactttggg attttctttc tttatctcct
    37681 cagtcaatgg gttctcaatg cgaatacctc ggctcaatcg aatcatcatc ggtgacaaat
    37741 gatcgagtag tccattgaaa agctggagat cctctactaa tgaaaaatga aattcctgag
    37801 acaatctttt cagaactttc gctaattcct ttgatagttc ctgatcattt tgattttctt
    37861 tgtgattcga cttagccatg agatgtaatg tcaaataagc aatttcttct tcgggaaacg
    37921 atacacctgc gattacctca atacgttgaa cgatccgctg cgccacttga tactctactc
    37981 gcttagaaac ttcttgttcg atgcctaatt ccttgatttc aaacccttct cggagccgtt
    38041 tgatccccaa agccaagtgt aaagtcagat tctgtatgat aaaatccgaa acttttaact
    38101 tagcttctct tgtttcatcg agaatgataa tgactagctc ttcaaaactg atgtctggga
    38161 aaaaacggct ttgccctaaa tattctttca atgaatttcc ataatggttt ccaaaaaaag
    38221 tatccatgat aaaatgccgt cgatttcttt cttccccttc gatccatgtc ccttgaccat
    38281 gcttagaaac gatttttagt gaatatggaa tcaatcgctc cttgatttct tgcatgtcct
    38341 ttgtcaaact ggaacgactg atataaagct cttctgccaa atcatccata aaaagaatgg
    38401 catcttccaa aagcaattgg ttcaatatat acttttgtcg atcttcggac tcataaaatc
    38461 cttgcggttc tctagcatat ccaggtacta tttcttgccg actcaaaaac aagtcaaatg
    38521 tcagtctttg taaaatatgc agttggtagc cataacctgg tttagcaatg atcttacctc
    38581 cgtttttttc tactaactct ttgatccgct gcaagtaatt acggattgtc cgatcagata
    38641 ataacaattc agaagctaaa cgttgactgg tgacatactc cttcttgtga tcaatcaaat
    38701 atctcaacag caatttttcc ttctgtttca ttatcatcca ctcctattcc atcatatgtt
    38761 ctcttcgatc acctctgcta acttctggat gcctgtaggg attgggacat acgcttggaa
    38821 agggatacta acaacaggct ttccaacaga ttctcctaat tttttgaatt tatccaaaaa
    38881 catcgtcgtt tgcgggctga tcagaaataa atcaaaatca ctattcttaa tcatattatc
    38941 gccttctgta gctgaaactg catctacgag tacctcttct cttttttgct taaagaaatc
    39001 cgttgtcttt gaggccatca tcgaggatga cattcctgcc gcacaaataa tcaatgcttt
    39061 tttcataaat caatcactcc ttttattttt ataaaccaag catatcttgt aagcgttttc
    39121 tattcaaaat atatatttcc gtagcattgc ggaaaaaatg tgtatcatat cttgaataat
    39181 aatttctggc accgtgatat actagtgata gaattttttc aggaggggat ttagtgagta
    39241 tcacagtgaa tattttgtat acaagtacaa atggacaagc cagaaaattt gcagaagaaa
    39301 tggtcgaaag aaacattgtc aaagcgattc gtgcagaaga aggaaacgag aaatatgact
    39361 attactttcc actagaagat ccagaatctc ttttactgat cgaccgttgg aaagatgaag
    39421 aagcaatcga aaagcatcac aaatccgaaa tgatggctca aataaacgaa ctccgtaaaa
    39481 agtataaatt aaaaatgaaa gtcgaacgtt tcactgattt accgtaaaaa gatacacatg
    39541 gaatgatagc aatttatcat ccatgtgtat cttcactttt tccttatttt ttattcgatc
    39601 tcatctaatg attcaaaccg tgtttttctc ttatttttcg gataagtatt tcagtcaccc
    39661 caatatacat gattcctaaa cataccccaa ggatattctc aaaaatgcgg atcgttactg
    39721 ctcctggaac cccgaataaa ctggcagtga ttgccaaagc accaaaagaa ttaaaaactg
    39781 ttttatatct ataggtagaa caaacaccta aagctagacc acctaatata ccgacccagt
    39841 taaaaggaat aaattggctg ataccaataa ataaaacaca tccaatcaat gagccaatga
    39901 ttcggtctac tgctcgttct ttgaaaccaa tcgacatcaa tccatagcta gatacaatag
    39961 aagaaaatgc aaaccctgcc cacatcaatc gctgaaaagg gacatactct cctgcaacga
    40021 tcaaaagact aatgccaaat gcatagatga gcatccaaat tttttcctgc gaatacatgc
    40081 ctttcaaaaa aatctcttta aacaagcttt tgtctttgtt cttttctcga tgttttctgt
    40141 aaaggagaac tgaaaaccaa caaaaaaaca aaaataataa gctacttgtt tgtgtaaaga
    40201 aattcttgtt caacgaatct ttaggtaacg aataaacaat aaagagatat gaaaagccgt
    40261 acaaagaagc attccccatt ttgggatcac tagcggttgc cattaatatg ctgcttagta
    40321 aaataaaatg aatcaacatg cccataactg accatttgat cagctggaca tacggtgcta
    40381 ctaatagtga gaacatgacg attgctaaac ctatgatcga atgggaaatc ttataaccaa
    40441 agtcaacaaa acgaatactc atcaacatac agaataaaac cacagcgtat ggtttatgct
    40501 tcgattcaaa taatgttgta attataatga taaaacaaat ggcaaacaat acgatgaata
    40561 atgatcgtaa aaacaatgaa caaatataca tgcacttttc ttttttagaa gctgcttctc
    40621 gtattttttg ttttaaaata aacggatcta gttgtagaga ctgataaaaa tccacctgtt
    40681 gtcgtccttt ctttttactt ttatttttta cagtcatcta tgacaggttg taaatactat
    40741 ggaacatcat atggcgttgt cattttcttg tcaactattt ttcctattca tgtttttata
    40801 aaaaaacata tcgttttctt gtatgattaa atcaccacaa aataaacaac tgagagaacg
    40861 atatgcttta tgaatggttc tatcaaaaaa attctgagat caatagacaa aaatttgatg
    40921 attacagagg tctcttacga aaccttacag aagaaaaaaa cattgatcgt tgctgcagtt
    40981 ctcttccctg ctccatgtgt ctgtagaaat tgtggttctg ctgtggtaga tggaaatagg
    41041 aaattaatgg tggtgaaaaa caagaagaag gaaaccattg tccactttga ataatataat
    41101 catatgccaa tggttatacg cctaaaaaag cagcgctata cctgtaaaaa ctgtcgaagc
    41161 cattggaacg ctcaaagtta ttttatccgt ccaaggcact cgatttcaaa tcatgtcaga
    41221 cataaaatca cttctttact gactgaaaaa gtgtcgttat tttttatttc gaaaagttgt
    41281 taggtatctt tgacaaccgt tattcgcaca ttgaaagagt tgagaagcta tttaccaaag
    41341 tcttccaaaa gaattctgtc ttgagtgttg agggttgtga atttcgttct cacgtttccg
    41401 ttgaggataa actgagtttt atttccgcgg atggcgagac agaaaaatta atcgagaaaa
    41461 tttacctaca caaaaaattc ctcgattaac aaactatttc cttagctgca ctaatcctaa
    41521 agaaatagaa ttcttagtta cagacatgaa cgccacctac tttcagctca ccaaacatgt
    41581 tctaccaaat gcgaaattgg tgattgatcg attacttggt ttttccacgc ctttcaagga
    41641 ggcgtaacct ttattcacga gttggttgaa gctttccgag acaaaagacc cgacttgttt
    41701 ttctccttat tgacaaagag ccattaaaac tagagaaaaa attacaactg atcgctgatt
    41761 atttgtagaa agaaaatcaa agtattaccc aataaacttg agtcattagg caaaaaacac
    41821 tgcgaaactc aagaatgatt ttcgcagtgt ttttgtatat tttttattaa ctagtattac
    41881 tacaaaggtt tatttttcac ttctcaacgc agcacaaaat tgtctttact tatttactgg
    41941 ttgggttcaa ccttctctac tccgatacta atatttgttt gtatacccct tctaaaatcg
    42001 gtagtttcca ttcaattgat gggtactagt tgattttaaa aactatgctt attcaatttt
    42061 ttattttagc tcatatattt cgtttctttc taaattttat actgatgaaa acaagtagca
    42121 cacccaatat tgacaatata aactggctaa tgatggtttc tccagttttt ggaagatgct
    42181 tatttccact gcttgttgga ttgtttcctc ttatatctga gctatcagca acttttctat
    42241 tctcttttgt taaaaagatc gccacttctt gttctttttc gatcttgaaa gttactggtg
    42301 tcgcatccaa ttcatacccc gttggcgctt gagtttctac cagctgatat gctcctggtg
    42361 ctaagccatc tatcgccagt tttccatctg ctcctgtggt tagacctgac tgcagaacgg
    42421 ttccattggc atcttgtaac tcaaaaacag ctccttgcag g
//
LOCUS       DAZKLD010000008        40756 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.008, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000008 DAZKLD010000000
VERSION     DAZKLD010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 40756)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40756)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40756
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.008"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            129..1270
                     /locus_tag="ACG8KR_000359"
                     /pseudo
     CDS             129..1270
                     /locus_tag="ACG8KR_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318369.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
     gene            complement(1396..2475)
                     /locus_tag="ACG8KR_000360"
     CDS             complement(1396..2475)
                     /locus_tag="ACG8KR_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFQ0199762.1"
                     /translation="MNNVFIFCYLEVVDLTVYQRFLANEKIRRLTVLLFVVFVLFLVR
                     SMITTILLTFIFTYLMIHLVQIIQRFVRIPTGVLAIMIYTLIVYLIYLAFTKYIPMLI
                     HQTFDMIQSVINFYEHQPKDADPVLQYIHTYIERNDFFEQLQNGASIALGYLQDIGKM
                     AVAFAMSFILSFFFMIEKKKTIVFSRLFLKSEFSWFFQDIYYFADKFVNTFGLVLEAQ
                     FVVALLNTLLTTIALAVFGFHQLLSLAIMIFILSLIPVAGVIISCIPLSFIAYSQGGI
                     RDVVYILITILIVHLIESYVLNPKLMSSKTELPIFYTFVVLLVSERFFGVWGLIVGIP
                     IFTFFLDVLKVKKIPNHLPKETDIP"
     gene            complement(2578..3018)
                     /locus_tag="ACG8KR_000361"
     CDS             complement(2578..3018)
                     /locus_tag="ACG8KR_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFQ0199763.1"
                     /translation="MEDLYLVNQVCFPLYAASKEIIRHYTPLLKPLNLTYTQYLVMLV
                     LWEKKKTSVKELGNDLCLDSGTLTPLLKKLAEKNYINRQRETGDERIVNVTLTAQGAA
                     LQKQAEGIPQKIKSYLPLEQEELDTLQHLTRKMLDNFHQDQKKG"
     gene            complement(3075..3485)
                     /locus_tag="ACG8KR_000362"
     CDS             complement(3075..3485)
                     /locus_tag="ACG8KR_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HFQ0199764.1"
                     /translation="MKKMYSTKMINTGGRSGEVHNPDRTFELNIAAPGKRVENATNPE
                     QLFAAGFSACFNSALELIKQQKNISGASTVSAQVSLYSESETSFVLGVEIEGSIEGLS
                     LEETQELLEAAHKVCPYSKATAGNIEVTLKAVSE"
     gene            complement(3665..4099)
                     /locus_tag="ACG8KR_000363"
     CDS             complement(3665..4099)
                     /locus_tag="ACG8KR_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFQ0199765.1"
                     /translation="MHKGTEIDFVLNDSLKALELYQTIFDEIQLIEATDYPRGNNEVI
                     FTLYGTRFHMLDENTSLGLTAPTAEHPNTIWFNIIVSDIEDTHNKALRNGCVEIQEMT
                     EMMDFGVTNSMFRDPFGYLWMLHQVHREVIFEERKQLFEKYM"
     gene            complement(4199..5047)
                     /locus_tag="ACG8KR_000364"
     CDS             complement(4199..5047)
                     /locus_tag="ACG8KR_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFQ0199766.1"
                     /translation="MKETRQFIWTYIKIIFALFILGVAINMFLGPHHIAAGGVSGLGI
                     LLEAALGFDRAIVILVLNIVMLVLALLFLGKKPFFKVLFGSLVFPLIIAIVPETMITS
                     DRLLSVIFGSAIFALGVAILYKNNSSSGGTTIPPLIFKKYFHLNTSIGLFFTDAFVVS
                     LNLFIFGIEEFLYAILSIAITSIVMTYIETGINRKKSVMVMSEHYLEDIRLRLSAEIG
                     RGLTLLEAKGGYNRKSKEVLLIVVTDHEFSRMKPLIEEIDPSAFVIVSSVAEVMGRGF
                     TYHPIE"
     gene            5436..7202
                     /locus_tag="ACG8KR_000365"
     CDS             5436..7202
                     /locus_tag="ACG8KR_000365"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HFQ0199767.1"
                     /translation="MDTAAIYHRPESEYAFLYTNTRFRIRLRTRKNDIKKVYVLCGDP
                     YTITTEKWYQKQRPMKKCLSTNVHDYWEIEVTSETSRLQYAFHVVGEDNTDCFYGDQG
                     IFPYRENVITEPNFYFRIPYFHQIDRFTIPEWVKETVWYQIFPERFANGDKANDPEHT
                     LPWGSKDPDREDFFGGDLQGVMDHLDYLTDLGINGIYFCPIFKATSNHKYDTIDYYEV
                     DPAFGDKKLLKNLIDEAHKRGIRIMFDAVFNHMGVHSPQWQDVLKNGEKSIYKDWFHI
                     RFFPVDSYQMTDLPETAENIPYDTFAFTPFMPKLNTANPEVQKYLLDIATYWIKEFDI
                     DGWRLDVANEVDHHFWKKFREAVTEIKPDIYILGEIWHSSQAWLQGDEFHAVMNYAFT
                     DSIKDYFAKKKITASQMVSGMNHQQMLYRDQVNEGTFNLLDSHDTARILTLCQGNKEL
                     MKSVMAFMFLQKGSPCIYYGTEIGMTGEDDPDCRKCMIWEKEEQDLELFGFIKELVSL
                     RKQLSKIISEGSTQWLIVNDREDKLYFTRKLEGQIIYVYFNQSKEPWVVEQENEVILS
                     QNCQLLEDGKAEIRQYGFLIMC"
     gene            7304..8563
                     /locus_tag="ACG8KR_000366"
     CDS             7304..8563
                     /locus_tag="ACG8KR_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFQ0199768.1"
                     /translation="MKKRSVKQLGLGLISLGILGGLAACGNGNSSAEGEADEGKTLTV
                     SVDSGYKDYINEIKNTFEKENDVKIKLVEKDMFDQLESLALDGPAGKGPDVMMAAYDR
                     IGALGQQGHLAEVKLGNEAAYDETDKAQVTYDGKIYGEPAVIETLVLYYNKDLVDTAP
                     STFKDLENLSKDSRFAFESEAGKNTGFLAKWTDFYYSYGLIAGYGGYVFGDDGTNPSD
                     IGLNNAGAVEGISYATDWFKNVWPKGMQDIKSAGDFASQQFMSNKTAAIIDGPWQAQT
                     YKENNINYGVAKIPTLNNGQPYQPFGGGKGWVVSNYAKNKDLSQKWLDYVTNQENQEK
                     FFEMVNEIPANQQARETAKAKNDELTTAVIEQYETAQAMPNIPEMGEVWTGAENLMFD
                     AASGSKTPKESADEAVKTISEAIEQKY"
     gene            8710..10011
                     /locus_tag="ACG8KR_000367"
     CDS             8710..10011
                     /locus_tag="ACG8KR_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0199769.1"
                     /translation="MQQDKTSNINEKKITLCSLIPGMGQFVNGQAFKGIVFFAIFVLF
                     IGQMLLGGFHALVGLVTLGSIPMEDHSLFILIEGTLQFIITIIFLIFYGLNLLDAYRV
                     AKMRQEGKKINRTMKEVIHNIGDEGFPYLLTLPAYLLMIFTIIFPVLVTLFTAFTNYD
                     FNHIPPASLIDWVGAKNFFSIFFLSSYRNTFFAVFSWTVIWTLCATTLQITLGILTAV
                     VAHQKFIKFKRIFGIIFLLPWAVPAFITIMSFSNIFNDSVGAINTQVIPFINQLPFVD
                     ISAISWKTDPNWTKVAIILVQGWLGFPYIYVMVTGILQSISEDLYEAAKIDGANAWQR
                     FSNITLPAVFLVAAPTFITQYTGNFNNFSMIYLFNNGGPGSVGGGAGSTDILISWIYK
                     LTTGTSPQYSMASAITLIISVIVICVSLLVFKKTNAFNMGD"
     gene            10014..10865
                     /locus_tag="ACG8KR_000368"
     CDS             10014..10865
                     /locus_tag="ACG8KR_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HFQ0199770.1"
                     /translation="MKKFHSSARFQKRLSHFFTYFFLLILSVIIIYPLLITASTAFKS
                     GNIAAFSLDLSSDWTLGNFTRLFSETLYGTWYKNTLIISVLTMIVQVTVVTLAGYTYS
                     RYRFIGRKSSLMFFLVIQMVPTMAALTAFYVMAFLLEALDKYWFLTMIYIGGGIPMNT
                     WLMKGYFDTVPIDLDESAKLDGAGHFRIFFQIVLPLVKPMLAVQALWAFMGPFGDFML
                     AKFLLRTPENQTVAVGLQTFISDVTNQRVSLFAAGAILIALPICLLFFFLQKNFVTGL
                     TSGGTKG"
     gene            10878..11693
                     /locus_tag="ACG8KR_000369"
     CDS             10878..11693
                     /locus_tag="ACG8KR_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrose utilization protein MalA"
                     /protein_id="HFQ0199771.1"
                     /translation="MQTFPLNYFSALRTPTQVFAGRKLLSWPKFFLIFVFLVSLMVMP
                     VTLFYANQIQAIPLEQFLSVHSLIDEQGTQKFSELELSETGLQASQQTIAVTPEILVG
                     VSLSEKQQSDHGTFIDFEKEQWVIQQKDKSGIRRYTMNYSPSFQPDSVRTPEDFQRFL
                     EREFYASNRPTIILSYSLSLGLVLFVMTSLILFGASFFLWMTRKSQLSSIHTFKESAN
                     LMLNVMGIGSMAAAVVGFIHFDFILMLSVQTTITVLLLLWVFVKTRFKDKRVE"
     gene            11844..13010
                     /locus_tag="ACG8KR_000370"
     CDS             11844..13010
                     /locus_tag="ACG8KR_000370"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFQ0199772.1"
                     /translation="MLEKTLYNQLFSRSFSLPVEVNYWDGTTKQYGDTDTPPQIKINI
                     HEEIPMKEITSNASLALGEAYMDHRIEIEGSIQALINDAYQQANSFLRGKDYLKWLPK
                     KEKHTKQKNKEDIHAHYDLGNDFYKLWLDPTLTYSCAYFNTPEDTLEQAQINKVHHIL
                     DKLFIKEGETLLDIGCGWGTLMFTATKEYNVKATGITLSEEQYDLITKRIKEEHLEDK
                     CRVLLMDYRELKGETFDHITSVGMFEHVGSENLEGYFKVVKDLLKPKGTALIHGISRQ
                     QGGATNAWINKYIFPGGYVPGVTELVDNITKNDLQLIDLESLRRDYQLTLEHWTKNFH
                     QVQDQVTKEMGDSFYRMWDLYLQACAASFESSNIDVIQYLLVQPGNNDIPMHRA"
     gene            13240..13887
                     /locus_tag="ACG8KR_000371"
     CDS             13240..13887
                     /locus_tag="ACG8KR_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HFQ0199773.1"
                     /translation="MKKTVIYTSLFAALFLAACQSNTNQGTSGNTSSSSEQRSSQVSD
                     SSTKASTSSSSQTKSSSEASTQSDIKVSAARAIELFQDKYPDTAITSLELDSDWGSYF
                     YKIEGVDDQKEYEARIDAQNEKMETENPETLDRDEQNGQKKKEDGLDVDKLITIEEAA
                     KIAVDKVGSGTATDWDLDKDLGTTYWEVKVKDGNQSTSVKINSQTGEVLSTEQDD"
     gene            13971..14915
                     /locus_tag="ACG8KR_000372"
     CDS             13971..14915
                     /locus_tag="ACG8KR_000372"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HFQ0199774.1"
                     /translation="MSLETTFANHIFANPLMNASGVHCMTTQELDELAHSEAGAFITK
                     SCTINERKGNPEPRYFDVPLGSINSMGLPNLGFSYYLEYALAYEKVQENQNQPLFFSI
                     AGMSVQENLEMLEKIEKSGFNGITELNLSCPNVPGKPQLAYDFEATYETLKEVFSIFS
                     KPLGIKLPPYFDFAHFDQMADILNQFPLTYVNAINSVGNGLYIDTEQEAVVIKPKEGF
                     GGIGGEYIKPTALANVRAFYTRLKPEIQIIGTGGIRTGQDAFEHLLCGASMLQIGTEL
                     HKEGPEIFSRIIKELTQIMSEKGYTSIDEFKGKLRTIS"
     gene            15050..16501
                     /locus_tag="ACG8KR_000373"
     CDS             15050..16501
                     /locus_tag="ACG8KR_000373"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HFQ0199775.1"
                     /translation="MNDATELAKELKEKEFSFQELVTDIQKKIKKQNHELNAFVALEP
                     FESNSHELLIPQSEKGPLAGIPFPLKMLGQEKKGWLATSGSRLFETHRASHTSNYVRQ
                     AEAIGLVPFGQTNAPEFGFKNITDPVIYGPARNPWNLECTPGGSSGGAAAAVSSGIVP
                     LAGASDGGGSIRIPASFCGLIGLKPSRGTMPVGPYAWRGWQGAAIDFGLTVSMRDTEA
                     LFYGMRSIHSGAPYQVSPVEWQTHPRKKRLKIAFCVDSPIHSQISEEAVLAVKKAVDF
                     LGKEGHDLTEIPYPLDGRKLITSYYQMNGAETAAMIRSIETGIQRAVRKEELEPISWT
                     LFQYGQKLSAATYIDSLHVWDEAAIVMENLHQEYDLFLSPTTAFTAPKISEDLQSDAI
                     RAQMEHISDCSEVEGLDVVSSMFEESLKITPYTQLANLTGQPAISLPTHLTKENMPLG
                     IQFMAARGREDLLFQIGYLFEANHQFHLPNPWK"
     gene            16522..17358
                     /locus_tag="ACG8KR_000374"
     CDS             16522..17358
                     /locus_tag="ACG8KR_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HFQ0199776.1"
                     /translation="MKKKRLFALITSAFFILSACGTAAANQEDNSWSNVEKKKELVVA
                     TSGTLFPASYYNDQNELVGYDVDLAKEVAKRLNVKISFKEYNVDGQITSVSKRESDFA
                     ANDFGITKEREKKFSLSETFKYSFDSMIVRKKDDSGIHSLEDLKGKKAAGEPNTSYMR
                     LAEKHGAKLVTYDNATNDQYLSDVANGRTDVILNDYYLQKMAVSALPDIPVKILENVY
                     FNENESGFLLKEGNDALKKKIDGVLKEMKEDGTTKKISEKYFQTDVSVKPKETMAESV
                     SE"
     gene            17358..18017
                     /locus_tag="ACG8KR_000375"
     CDS             17358..18017
                     /locus_tag="ACG8KR_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFQ0199777.1"
                     /translation="MYFPLIDFSLMWTSLPFVLQGLVYTLGIGFVSFLLGNLVGLLLT
                     VLGLLDWLPLNVFIRFYLSFFRGIPALVLLFLLYFGLPYQLSALTASVICFTITSSAF
                     IGEIYRGSLAGVSSGQWDAGLASGFSFFGVLKYIVLPQAFRISVPALSNVAMDLVKGT
                     SLAATITVPEIFQKAKIIGGRTFDYFSLYILVALIYWVICIGIEKVQQYLEKRSMHQS
                     I"
     gene            18143..18832
                     /locus_tag="ACG8KR_000376"
     CDS             18143..18832
                     /locus_tag="ACG8KR_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0199778.1"
                     /translation="MKTPLTIEIESSLYQYCIEQGGLVVEEVTMPDDQGIVDTLSCLT
                     KFDGSREWRCYELKVTKADFRSTAKLSFVGHYNYFVLPSKLYEEVKTEIPTGIGVLVY
                     RPYTVEEEMPASGTFLIAKKAAKKELAVPEEALTNRFMASLFREVRKAKQMERGVKYF
                     PTDQLYKELKRRSENQDPFSNERYYERFLEEIASQAINELQEEVIALQHDYEYLRQQQ
                     QIRRLPTEPLE"
     gene            18838..19794
                     /locus_tag="ACG8KR_000377"
     CDS             18838..19794
                     /locus_tag="ACG8KR_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HFQ0199779.1"
                     /translation="MYYPYLRGKQFDLLALKEALSRGLLSNKIQPVIEPVRDSATLKN
                     VIELFQKKHHRIAVIQNPQVGQFKLFDQHVHSWTVKENSSVVPAQILTPENLSEVLDS
                     PPAFLVFDGQHYPKDTEVWKQLAGVDSKFLIPDTSRFRIWLPENKIVIRDSFQTRKHV
                     ESYADKTDDFFSDDYLFFHADGYIGFSDFTIEGSRYFDKGFPSRALAIHLTYIDAYGN
                     IRVKHFVSDSNDSAKDQALKFLEAGDKMKKWIMRHHHQLLITSGMIELLALYQQQKFP
                     GLGVLKKWSLMHHLELISHLLDHPKDWLKYYSKVQELYEVIQ"
     gene            19838..20350
                     /locus_tag="ACG8KR_000378"
     CDS             19838..20350
                     /locus_tag="ACG8KR_000378"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0199780.1"
                     /translation="MYIRQTRKEEVEEVVEIINAAADFIASQGSPQWQNGQKPTKETI
                     LNDIERKESYVLIVEDQIAGTAALVSSVDPVYTKIDGSWKESTGPYLSIHRVALAEST
                     RGKKLAKPFLEHLLTIAASKNIQDIRIDTYPANEPMEKTIYSAGFTYQGMIEFPFEHG
                     ERKAYQIIIN"
     gene            complement(20606..21154)
                     /locus_tag="ACG8KR_000379"
     CDS             complement(20606..21154)
                     /locus_tag="ACG8KR_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HFQ0199781.1"
                     /translation="MNYCYVFIGPSGSGKTSLAEAIFQPEQKIITYTNRSPRPGEKDQ
                     EDYYFVSDNTFNQMIKGNEFAEWDQYAQHKYGSSKAEIKKKLTVGNCYAVLTANGFWH
                     LYEYFGTVVRPIFITISKETLQSRLKKRGDSSEQISARLAVYVEDLAQLKRLKAIPSL
                     LVVSNDGAFQEAADKIKKGLLI"
     gene            complement(21243..22424)
                     /locus_tag="ACG8KR_000380"
     CDS             complement(21243..22424)
                     /locus_tag="ACG8KR_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside transporter C-terminal
                     domain-containing protein"
                     /protein_id="HFQ0199782.1"
                     /translation="MSYLLGIIGLFVTLGLAFLISSNKKAIKLKPLIVMFVLQLVLGF
                     VLLRTTFGTAVVSVLAKMFDHLLAFAGQGVDFVFAGVANKGSAPFFLSVLMPIVFISA
                     IIGILRYIKILPLFMKAVGLGLSKINGMGKLESYNGVASAILGQSEVFISIKKELPFL
                     SEKRLFTMSVSAMSTVSMSIVGSYMALIDSKYVITALVLNLFGGYILASIVNPYVLEE
                     KEDELIIEENKEQTFFQMLGEYILDGFHVAITVAAMLIGFVALIAMINAIFHGIFGIT
                     FQEILGYIFAPLAFISGIPWKEAVDAGSIMATKLVTNEFVAMTELANGNFHFTERTTA
                     IISVFLVSFANFSSIGIISGAMKGLNEEKGNLVAKHGLKILLTATLVSFLSAIVTGLL
                     V"
     gene            complement(22660..24168)
                     /locus_tag="ACG8KR_000381"
     CDS             complement(22660..24168)
                     /locus_tag="ACG8KR_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HFQ0199783.1"
                     /translation="MKKIGFDSEKYIEEQSAYILERVNHYDKLYLEFGGKLVDDKHAK
                     RVLPGFEEDAKIKLLQKLKDQAEILICVYAGDIERNKIRGDYGITYDMDILRLIDELR
                     GYGLLINSVVITRYSGQPATKVFINKLERRNIKVYKHSEIEDYPVNVEKIVSEDGFGK
                     NEYIETTKPIVVVTAPGPGSGKLATSLNQLYHESQKGHAAGYSKFETFPVWNVPLKHP
                     LNIAYEAATVDLKDVNMIDSFHFDAYNKVAVNYNRDVETFPVIKRIIEKITGKESVYQ
                     SPTDMGVNRVGFGIVDDTVVQEASKQEIIRRYFQTECDFKKGLIDEEAVNRIKLIMEE
                     VGLRPEDRNVVVPAHDYADKIQAQSPTKDPSAVIAIELPDEVIITGRTSSLMDASAAV
                     ILNAVKHLAHIADDIPLLSPLVLETIQGLKSKTLHSSLDALSINEVLIALSISAVTNP
                     IAQVAYDKLAELEGAQAHSTVMVNKNDEQTLKKLGIDITCAPVYPSENLYYQ"
     gene            24327..24518
                     /locus_tag="ACG8KR_000382"
     CDS             24327..24518
                     /locus_tag="ACG8KR_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0199784.1"
                     /translation="MKKRQKKKNAYKHYIRSIFTGYERMLEDPELEQLTFTYLNEETQ
                     LTRDEHQRIHFTTRDLPSK"
     gene            24721..25638
                     /locus_tag="ACG8KR_000383"
     CDS             24721..25638
                     /locus_tag="ACG8KR_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coiled-coil domain-containing protein"
                     /protein_id="HFQ0199785.1"
                     /translation="MKEPDTNANYEPDELEETNADQDTGQESSSFYDIKEKASMNLSQ
                     LLEDLQAFEKAVREEDIPEIYRLYNGRLNEELKRSSNENHEIDQLLMRKIHDRFLQEF
                     PFMEQVENISPTMSYYKIGTYYHDRPTVGIDASLPEIFVLPKIDEEWEKYSQPESTDY
                     DKKINELDAKVITAQTEIERLDEQIKEINRQMTDLNDNKGFLNRKKIEEEIQELEKKK
                     QVLSNEKLGWLPYIETPETIQQQKEALKQEARADQLRAAIVEKEQRQIRRYFGSKEGF
                     GQAIHEFLMNYLGNENPNNQSNEGGSEYE"
     gene            25631..26704
                     /locus_tag="ACG8KR_000384"
     CDS             25631..26704
                     /locus_tag="ACG8KR_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0199786.1"
                     /translation="MNEHDEAEWTKNEEQTEDHEETNTSLEEQTSNPLDDTEEIPMLE
                     ETKIHEKEKVTPTNTNTAENTHAYEITENPGYLEGKEQDHQDIVSLIETAEAELADLR
                     MRKDLIESDFPQLLESYDRFILNEEPISTIGKTSLIEYFTYELLKPLNDIGLERSNSA
                     YDTWKTRHFSINYTLNGQNELVFSFVLPTIDQRYQVESMSLLNVSPETMEINVQDDRV
                     LSLIRHWSVDRVFSAGQITIFNHKLNQLLAHARTLGFTVNQTLLDNTKPLRLNLQSEF
                     ELTNEVLDDIFIVAMKNPSYDMEKINETTYQVRLDKGQSLTITTNEKDQTVLSISSGD
                     YPRSVIDFFINYEFLVPLMVRKS"
     gene            26852..27370
                     /locus_tag="ACG8KR_000385"
     CDS             26852..27370
                     /locus_tag="ACG8KR_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0199787.1"
                     /translation="MSTNKIAQLSLLSAACVAGRIAFQFIPNFQPMTAIFLFIVLYLS
                     LKDALIVMSLSIAISSFYFGVGPWVIGQWISYTVVLSLFSIVCLRKTVQRNLWLKGVC
                     FFLAGMVYGIGMTVFDTLLYRLPQPWIYYLQGVSFDLMHSIGNVVFFLVFLPVVKRFY
                     NHSGGKNEKNNL"
     gene            27351..27743
                     /locus_tag="ACG8KR_000386"
     CDS             27351..27743
                     /locus_tag="ACG8KR_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HFQ0199788.1"
                     /translation="MKKIIYSLLAMSVSILILTSCSTTYTQSASNQKEEVKTSQKATI
                     TLQENGQNFSEKEVFFKKGDDLLSILKRNFEVKEDNGFITSLEGHSQDEKNQLYWMFT
                     VDGKSATTGAKEIKLNDGQSVIFNLSKL"
     gene            27819..28754
                     /locus_tag="ACG8KR_000387"
     CDS             27819..28754
                     /locus_tag="ACG8KR_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFQ0199789.1"
                     /translation="MENFKKSGMFSVIAALGANILVAISKFVGYAISGSAAMLNESIH
                     SIVDCSNQILLLFGDKQANKGQSELHQFGEGRAKYFFSTIVAMMLFFGGGALGVMEAF
                     EKLTHPSHEVGNPIIVIAILLFGLVIEGSSLRVAMKEIKELNTEKLSTMRFLRESRHS
                     EILIIFTEDLCAVIGLVLALAGTILTYATGIAAFDAISGLLIGFLLMGAAIFLAKEFY
                     SLIIGESVTASDLSKIKLAFERPEISRLIDIKTVHLSPTEILVAAKADIVGNKENEAY
                     SVINDIEKNMRQSLPDKKMYIYIETDKYDPNYSRK"
     gene            28887..29666
                     /locus_tag="ACG8KR_000388"
     CDS             28887..29666
                     /locus_tag="ACG8KR_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HFQ0199790.1"
                     /translation="MNKKKSAPWLQALHAVMPLCISYIPVGLACGVLLQKVGFDPLLS
                     GLLSILVFSGGAQFLVASMLTTQASFATTLLMVFFLELRYTLLGASLAGFMKKEKRTF
                     LAVFSQSLNDENYAVNYLKFSTDPSWNKRKALYVNWFSMTAWAGSNMIGNFFGSVIRV
                     DADLVHFALTAMFIFMFIMQMKSALLIFTGLFSGVLGVVFMVLFQNTFGLIAATVIAS
                     FAGFMLEKAFKKEKAKKLRDRGKDVNEPLFNFPEQEANHHD"
     gene            29659..29976
                     /locus_tag="ACG8KR_000389"
     CDS             29659..29976
                     /locus_tag="ACG8KR_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HFQ0199791.1"
                     /translation="MTKLYLLLLVACLFIVAYIPRLFPMLYFTHRKVPSWFSDWMKYV
                     PVALFAALAFKDVFITHEHLDIAWNIKIAAMILVAGVAYKTRSMALSVLTGLASVFLL
                     SML"
     gene            30220..31830
                     /locus_tag="ACG8KR_000390"
     CDS             30220..31830
                     /locus_tag="ACG8KR_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFQ0199792.1"
                     /translation="MNKKLMQGTFWLTFANLLCKVLGVVYLIPWLSMMGNNQDGMLAT
                     TLYNVGYLPYGLFLMLGTVGFPNAIAKKVAVATKEGDNAACRLIFRSTINIMFVIGIA
                     SAALMYLFAPLLAGISPIANVNNGILAIRSLCPSLIAIPILSAMRGYFQGKNDLRPYG
                     TSLIIEQAVRVIVILAGTYYLRVLTDGTILEAVLISTVASFFGGLAAIIHMYIVGRRK
                     DFFELKDFLISSRYFERENRSASVSIIRETLPFIFVGSVITIVQMIDQVTMKPLLHFF
                     RPEIADQQLEFLFSRASVNPNKLTLILISMVGTVAISSLPILSTMKKKDRIQIEKTVG
                     DSFSIALLILLPSLTGMSLLAGPLYTLFFGYDPESVGYFQMALLASLFFSLFTILSTM
                     LQSLNHHRFAIRLTVEAIILKVIFQVIGLGLVGGYGMSLSNGLAFGIVFVRGYHFMCK
                     EYRIAPLAKISNFFLKTFRSTLIMLAVCFAVFFLLNQRLSMESKSYAMIYCVAVGSIG
                     GLVFLFSQFGKNGFRMLKNVKHHSRSKE"
     gene            31987..32835
                     /locus_tag="ACG8KR_000391"
     CDS             31987..32835
                     /locus_tag="ACG8KR_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFQ0199793.1"
                     /translation="MKLAVVTDSSAYLPERIKNHPDLFVIPIPLIMDGKIYNEGIDIE
                     ADEYYGLLKNSKDFPTTSQPVVGEVLGLYEELKEKGYDTILSIHLSSGISGFVNTLFA
                     MKDDISGVTVIPYDSKITSMPMGHMVEAALDLNDEGQSLDEILAHIDRIRDNTYAYLI
                     VDDLNNLVRGGRLTNGAALIGSLLKIKPILTFSEGKIVLFEKIRSSKKAFARAEEIIG
                     RRNNEIQPPVKLYVIHANNLEVAIAEKEKLQKKYPNAEIEIGHFGPVIGTHLGEKAIG
                     IAISAQ"
     gene            33066..33545
                     /locus_tag="ACG8KR_000392"
     CDS             33066..33545
                     /locus_tag="ACG8KR_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6530 family protein"
                     /protein_id="HFQ0199794.1"
                     /translation="MEIPTNLKHKPVIVVEDYDKVDGRNALNTDAKGLSLGLAQWNDR
                     GKVDISGKVWRHTGEKWSRQSEELPITRILDLAILAAQGSLYFQDAYRYEKFYDPENP
                     VVDIIGLQGNRMTVEVDTKNPKIDEDIILYYDTLQKDGELIGERFRILKRLLDELGY"
     gene            33578..35806
                     /locus_tag="ACG8KR_000393"
     CDS             33578..35806
                     /locus_tag="ACG8KR_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFQ0199795.1"
                     /translation="MENFSLTENRTFSRLEKQMIWKKPKSHRTSAEEWRIASEIKGNW
                     QDPEMKISNVLLEGDAGSGKTQLAKALSADLQLPYTKVTCFADMDKSDVFGALLPVVD
                     SNKEQDQELLEAIYQTDTLEAVLTLIQNHYGIDPFSAKEKLAELVQRIEADYPDAIHY
                     SYYPSEIVRAIEKGYLLEIQEPTVIRDASVLVALNSALEPNGMLNLPTGIIRRHPDCV
                     IVITTNRNYQGNRPLNESLRDRMQHAEKMDLPALEVMAERAMAKTMIQKQELLLKMAE
                     IIRLLDETAKANAIKGVAGMRSYFYWANTYKQGQDLLQSIYPKVLYKLSTDSDELAIL
                     EQALSDSGLLLELQDILRKEKWGHPQSEHTKGRTISAEEANERNITENAEEILRLDEK
                     KSLSAEEPNEGEEKQEETIEKNEIPPQMQKERSSDIEGKNQKEQQTGDDSQEMSASDM
                     EAHDKEIKKQLNKEARQIMKETIHEKEGLIVHRPAFQGKTPEARELHMQVMPIVESLS
                     RQILELLDNEQTETYQKGKYEGQRFNASKVAYGDLRNFDKKNPPHEQPSLAVAVRIDE
                     SGSMIRDDRIEAAKKAAIAIAEFSKKVDIPLLIYGDTADRSSREKTSLFSYKEFEDGF
                     QWLNEKLVTMKPRQNNRDGAALRLAAEKLNRQSATTKLLLNISDGQPKALPDYTGKKA
                     KEDIQSVLKEYERQGILFVSAAIGQDKEEIKSIYGESRFVDITDLNEFPKQMIQLIAR
                     YL"
     gene            35935..37578
                     /locus_tag="ACG8KR_000394"
     CDS             35935..37578
                     /locus_tag="ACG8KR_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HFQ0199796.1"
                     /translation="MIQEQKWWKEAVGYQIYPASFKDSNGDGQGDINGIREKLNYLKD
                     LGIGFIWINPIYQSPFVDNGYDVSDYQDIEERFGTMEDFDLLLKEAHELGIKVIMDLV
                     INHSSDQHQWFEESKKSKESPYRDYYIWVDGVDGKEPNNWTSIFGGSAWEYSHETGQY
                     YLHVFAKEQPDLNWESEKLKEELFNMIRWWLDKGIDGFRLDAISHVKKDEYSVKATEN
                     PFSPFQNVSGIEEHLTDLKHVFEEYDIMTVGEASGVTAEEGPQWVGKDGYFDMIFEFD
                     HIHIWQQEKEGQLDVLKLKLALSAWQTSLDGIGWNALYMENHDVPRAVSVFGDTRPDF
                     WAMSAKAIAMMYFFLQGTPFIYQGQEIGMTNMPFESIDQVDAVDSKRLYKRLLAEGKT
                     REEALDIIRETTRDNSRTPMQWTSEQYAGFSTHEPWLITNPNTKTINVEQQEYEPESV
                     LQFYKNMIRIRQTNKGLIYGSYKEYLHEHPQLYVYERYLEDEQYLIMVNLTESLADYE
                     LPKEADQSWTLLLSNSSSGEFEAKGILAPYEARLYKTNK"
     gene            complement(37651..38196)
                     /locus_tag="ACG8KR_000395"
     CDS             complement(37651..38196)
                     /locus_tag="ACG8KR_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HFQ0199797.1"
                     /translation="MKLSEKELKDQLTEIVNDILAEAHLKKGDLFVLGCSTSEVVGGH
                     IGKNSSAEVGQWIIRTLKELLDPKEIALAVQGCEHLNRALVVERTVAEAKNFEIVSVV
                     PALHAGGACSVAAFDQFNDPVEVEHVVGQAGIDIGDTSIGMHIKHVQVPVRPRLKTLG
                     QAHVTALRSRPKYIGGPRANY"
     gene            complement(38219..38704)
                     /locus_tag="ACG8KR_000396"
     CDS             complement(38219..38704)
                     /locus_tag="ACG8KR_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFQ0199798.1"
                     /translation="MKTKQLTIYAMLIALTVALSLTILIPVPATNGFVTLCEAGIYTA
                     AFLYGPTGGLVVGASSGLLIDLISGYPQWAVFSLVIHGLQGYVAGSFSKSSTRMWGLG
                     LLLGSLVMIIGYGIAGWILYDWPAGVASIPGNIFQNLIGMGLAFPITASLQRIQARKQ
                     Y"
     gene            complement(38694..39506)
                     /locus_tag="ACG8KR_000397"
     CDS             complement(38694..39506)
                     /locus_tag="ACG8KR_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HFQ0199799.1"
                     /translation="MKRILTIGGSDPFAGGGIQTDLKTFENFGLFGLSALTSIGALDI
                     NERFMLESISLELLKRQLVSIDKMTSLDGIKIGLLNSSEAIMVVRDFIKTKQNIPIIL
                     DPVLAFKETEAVINQLYISQLIEELFPLVTMITPNLAEAALLSGQALPSSLTEMIHLA
                     KELTASGTKNIVIKGGTRMAGDEAVDLLYSIDHYEVFSHKKIDTTTVNGAGCAFSSAI
                     TANLVLGADFTKAVAESKKFVAHCISNGVLMNDHTGSVWSGGIQKGGMRNEN"
     gene            complement(39645..>40756)
                     /locus_tag="ACG8KR_000398"
     CDS             complement(39645..>40756)
                     /locus_tag="ACG8KR_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HFQ0199800.1"
                     /translation="PILYLMEQPALSVSQSFLDKNEISYMTHTSGTTGIPKLICHSAH
                     SMGWRTKWQKTVFTKISEKKLIAFHISPVHSRFNIGISSLMSMGFPMMPLANAQSSKV
                     VHMLEAHRPIALETHPNNFVQWRFTAKEHPEAFSGIRYYHSTFDAINNQTMKTFLRTS
                     MAQDAVFLQVYGQSECGPMILKAHTLESLKTSDARDMGVGLEDMTSARITDSVGNVLP
                     ENTDGHIQLLSKGRALTYYKEDARFQENVYGDWWDSGDYGFMDKHGHLFLKDRQVDLI
                     ETINSTLAIEDFLLDSLDFLAEVVIVRDKNHSPQPIIALAPDKEMDWNRWWEQVHELP
                     RLNVPIIRDFDAIPRTATMKVQRLQIEKELKSQKS"
BASE COUNT        13535 a         7386 c         7968 g        11867 t
ORIGIN      
        1 ttaaattgtt aaaacttgaa aatattactg tttttttcat aaatttagac aaaaaaacag
       61 taaacatata ggtagaatct ctactatctt atttcttgca tacaaaaaaa tcaaggagtg
      121 agagagttat gagatattca accatcttaa tatcaacatt tgcagcctta agcttatcgc
      181 ttactataaa tcagacagtc cacacagagg agacagaaac agccaaatgg acagaaacca
      241 agtgggccaa cgtcaatcga attacctttc aggatgaatt ttcatccttc aattcaggag
      301 tcgcaaaatt aagaaaagga caaacagcga ccatcaaagc aaaaattgac tattcgggag
      361 acttagcacc tatactacaa tcagctgtta catttgagaa tgttgatccc actttgtcga
      421 ttggctatga cgagaacact ttgactatcc aacacaagca agcgatattt accttcaccg
      481 ttacattaaa tcaggacttg ttaaaaccag cgctttttac tgtcaaggta tcagatggac
      541 taccgaacga tcatcattta acgccgtata gtcagcggca gacaattgag caagattcag
      601 ccatcgactc aggaggagat cttgttgaag aaccaacaga taaaccagaa aatgaaaata
      661 agccagaagt accgcctaca gaaaatccag acggagaaca gaaaccggaa atagaacctg
      721 gagaagaacc cgatacagaa actcaaccag agccagacaa taaatcaaaa cctgaaataa
      781 cccctggaga aaaaccagat gtaactccag aaccagatac ggattctgga aaccaaacag
      841 taccggaaac aaatccagac acagataatg aaactgaaaa tccagaaaaa ccagaggtag
      901 atccagaaga aaaaccagat gtgactccag aaccagatac ggacgcaaga gaccaaggaa
      961 taccagaaaa aataaacaaa aaaacaattc aagaggatgg aaaaaaagag agtaaaaaat
     1021 caaatctagc aattctaaaa ataaatgaag agcagctgaa taaaaaatca cgaatatttg
     1081 atagcgccca atcagctgaa accttaaaaa gtagcaagga tacaaccttt gctgtcctga
     1141 aacgaaaaat aaacagctac caaaatccgg agagtcacaa aacaaagtta tactgtggag
     1201 cggaatcatt ctattaagta tagcaacaat gctttctgca aaaagattta aacaaaacag
     1261 atctctctaa catttgaata atgtctgtga acgcattttt caaataaaaa gaaatatcca
     1321 gaaaatgacc ccaaaatacc agattgaaat ctgatatttg gggtcgttca tactttatct
     1381 ttcttcatat ttattttaag ggatatctgt ttcttttgga agatggttcg ggatcttttt
     1441 cactttcaat acatctaaaa agaaggtaaa gatcggaatg ccgacaatca agccccaaac
     1501 gccaaagaac cgttcgctaa caagtaagac cacaaatgta taaaaaatcg gcagttctgt
     1561 tttgctggac atcaacttcg gattcagcac ataagactca attagatgga caatcaaaat
     1621 cgtaatcagg atataaacaa catctcggat gcctccttgg gaataagcta tgaaagatag
     1681 aggaatacaa gaaatgataa cacctgctac tgggatcaga cttaaaatga aaatcatgat
     1741 cgctaaactc agcagctgat gaaagccaaa tacagccaag gcgattgtag tgagcaacgt
     1801 atttaaaaga gcgacaacaa attgagcttc taataccaaa ccaaatgtat tgacaaactt
     1861 atcggcaaaa tagtaaatat cttgaaagaa ccaagaaaat tcacttttta aaaacaatct
     1921 tgagaataca attgtttttt tcttttcaat cataaaaaag aaacttaaga tgaatgacat
     1981 agcaaacgcc actgccattt ttcctatatc ctgtaaatag ccaagcgcaa tcgaagcgcc
     2041 attttgaagc tgttcaaaaa aatcgtttcg ctcaatataa gtatggatat attgtaaaac
     2101 aggatctgca tcttttggct gatgctcgta aaaattaata acggactgga tcatgtcaaa
     2161 tgtttgatga ataagcatcg ggatatactt tgtgaatgcc agatagataa ggtaaacgat
     2221 cagtgtataa atcatgatag ctaaaacacc tgtagggatt cggacaaatc gctgaatgat
     2281 ttgaacaaga tgaatcatca aataagtaaa gataaatgtg agcaaaatgg ttgtaatcat
     2341 acttctaact aagaaaagaa caaaaacgac aaataataaa acagtgagac ggcgtatttt
     2401 ttcattcgcc aaaaaacgct gataaacggt caaatcgaca acctccaaat aacaaaaaat
     2461 aaacacgtta ttcattatat gatggaatat gaaaataaga aagagcaaaa agaaaaaccg
     2521 ctaaatgacg ttatcttatg agttggtttt cctcataaaa tcgttcatct agcagtttca
     2581 gccctttttc tggtcttgat gaaaattatc tagcattttt cgggtcagat gttgcagtgt
     2641 atccaattct tcctgttcaa gtggaagata agacttgatc ttttgtggaa taccttctgc
     2701 ttgtttttgc aacgcagcac cttgagcagt cagcgtaaca tttacgattc tttcatcacc
     2761 cgtttcacgc tgacgattaa tataattttt ttctgctaac ttttttagta agggagtcag
     2821 cgtaccagaa tccagacaca aatcatttcc tagctctttc actgaagtct ttttcttttc
     2881 ccataaaact aacatcacaa gatactgggt ataggtaaga ttcagcggct tgaggagggg
     2941 agtgtagtgt ctaatgattt ctttagaggc ggcatataaa ggaaaacata cttgattaac
     3001 taaatataga tcttccattt ttccacctcc gctgttttac tatagtgtat catagcggat
     3061 ttacacaaaa aagtctattc actaactgct ttcagagtaa cttctatatt tccagctgta
     3121 gcttttgaat acggacacac tttatgcgct gcctctaata attcttgcgt ttcttccaga
     3181 gaaagacctt cgatagatcc ttcaatctct acacctaaga caaagctcgt ttcactttct
     3241 gaataaaggg aaacttgtgc actgacagta gaagccccag agatattttt ttgctgtttg
     3301 atcaattcta atgcactgtt aaaacatgca ctgaaaccag ctgcaaacaa ttgttcaggg
     3361 ttggttgcat tttcgacgcg tttaccagga gctgcaatat tcaattcgaa tgtacgatcg
     3421 ggattatgca cttctccaga gcgtccgcct gtgttgatca ttttcgtaga atacattttt
     3481 ttcataaaat taccaccttt aattatattt tatacaattg aattttacac aatataattg
     3541 tggaaagcaa agaaatggat tggcataaga taaataagag aaagcacatg aaacagacgc
     3601 gctttctcga atagcaaaca tatacaaaaa atatcagaga caaaaaataa tctcatcata
     3661 aacattacat atatttctca aaaagttgtt ttctttcttc aaaaattact tcacgatgga
     3721 cctgatgaag catccataaa taaccaaaag gatctctgaa cattgagtta gtcacaccaa
     3781 agtccatcat ttctgtcatt tcttgaatct ctacacagcc atttcgtaat gctttattat
     3841 gtgtgtcctc gatatcagag acgatgatat tgaaccagat cgtatttgga tgttcagcag
     3901 taggagcagt caaaccaaga gacgtgtttt catccagcat atggaatctc gtaccataca
     3961 aggtaaaaat gacttcatta ttgcctctag ggtaatctgt tgcctcaatc agttgtattt
     4021 catcaaagat tgtttgatac agttctagtg ctttcaaact atcatttaat acaaaatcga
     4081 tttccgttcc tttatgcata aagatctcct ctccttttca gtcgaacagt tactagattc
     4141 ttttattata ccagctaaag aaaacgcatt caaaaaaata gcggatttac ttaccaactc
     4201 attcgattgg atgataagta aatccgcgtc ccattacttc tgctacgctg ctgacaatga
     4261 caaaggcact tggatcgatt tcttctatca atggtttcat tcgggaaaat tcatgatcag
     4321 tcacaacgat caataaaacc tcttttgact ttcgattata cccgcctttt gcctctagca
     4381 gggtaaggcc acgtccaatc tcagcactta agcgaaggcg gatgtcttcc aaataatgtt
     4441 cactcatgac cataacagat ttctttcgat tgatacctgt ttcgatataa gtcatgacaa
     4501 ttgaagtgat tgcaatagat aaaatagcat aaagaaactc ttctattcca aaaataaaca
     4561 gattaagaga aacaacgaat gcgtctgtaa aaaataagcc aatcgaagta ttcaagtgga
     4621 aatatttttt aaaaatgagg ggagggatcg tcgtaccgcc gctagaagag ttgtttttat
     4681 ataaaatcgc aactcctaaa gcaaaaatcg cacttccaaa aatgacagac aacaaccgat
     4741 ctgaagtaat cattgtttct ggaactattg caatgattaa aggaaaaacc aagcttccga
     4801 aaagtacttt aaaaaagggt tttttaccta aaaatagaag agctagtacc aacataacga
     4861 tattcaacac taaaataact atagctcggt caaagcctaa agcagcttcc agtaatattc
     4921 ctagaccgct gacacctcca gcagcaatat gatgaggccc taaaaacata ttgatagcga
     4981 ctcctaatat gaagagggca aaaataatct taatgtatgt ccatataaat tgtctcgttt
     5041 ctttcattta ttcacttcct taattcatta ataatactct tatcttatta taacttactt
     5101 ttcaccttaa gttaacttta attaataaaa aaattaggct agtctaaatt attatgtttc
     5161 attcttctag aatatacttc tacgccaaaa actgttaaaa aacttttact ttagaaaatt
     5221 ggagaaattg atcgcttaag ataaattcag gacactaaat agtacaggaa gtagactaaa
     5281 acttttaaaa gttttaagtc tatctatttg gcaagtttaa aaaataaaaa ttcggtatca
     5341 aaaaactgtt accgataaca tttttgaaag cgttgtcttt tgcttctcta tcacttatag
     5401 tgatgacgtc atattaaaac acaaggagga aatcaatgga tacagctgca atttatcatc
     5461 gaccggaaag tgaatatgca tttctatata cgaatacaag atttcgtatt cggcttcgta
     5521 ctaggaaaaa tgatatcaaa aaagtatatg tgctatgtgg agatccatat accataacaa
     5581 ctgaaaaatg gtatcaaaaa caacgaccaa tgaaaaaatg tctaagtacc aatgttcatg
     5641 attattggga aattgaagtg acgagcgaaa caagtcgctt acagtatgcg ttccatgttg
     5701 ttggggaaga taacacggac tgtttttacg gtgatcaagg aatttttcct tatcgagaaa
     5761 atgtgattac tgaaccaaat ttttattttc gaatacctta ttttcatcaa atcgatcgat
     5821 tcactattcc agaatgggta aaagaaacag tatggtatca aatttttccc gaaagatttg
     5881 caaacgggga caaagcaaat gaccccgaac acactttacc ttggggaagt aaagatccag
     5941 accgagaaga ctttttcggg ggagatttgc aaggtgtgat ggatcatttg gattacttga
     6001 cggaccttgg catcaatggc atctattttt gtcccatatt taaagccaca tccaatcata
     6061 aatacgatac gattgattat tatgaggtgg atcccgcttt tggcgataaa aaacttttaa
     6121 aaaacttgat tgatgaagca cataaaagag ggatacgtat catgttcgat gctgtattca
     6181 atcatatggg tgtccattca ccacagtggc aagacgttct caaaaacgga gaaaaatcga
     6241 tctataaaga ctggttccac atccgttttt tcccagtaga ttcttatcag atgactgact
     6301 taccagaaac tgctgaaaat atcccatatg atacttttgc ttttactcca ttcatgccga
     6361 aactaaatac agcaaatcca gaagtgcaaa aatatttgct ggatatcgca acttattgga
     6421 taaaagaatt cgatattgat ggatggcgtc ttgatgttgc taatgaagtc gatcatcatt
     6481 tttggaaaaa attcagagaa gcagttacag aaatcaaacc agacatctat atccttggag
     6541 agatttggca ttcctctcaa gcttggctgc aaggggatga attccatgca gtgatgaatt
     6601 atgcatttac agattcgatt aaagattatt ttgcaaaaaa gaaaatcaca gcttcacaaa
     6661 tggtcagtgg aatgaatcat cagcaaatgc tttacagaga tcaggtgaac gaaggaacat
     6721 tcaacttatt agattctcat gatactgcca ggatcttgac cctttgccaa ggtaacaaag
     6781 aattgatgaa gtctgtgatg gcctttatgt ttcttcaaaa agggtctcct tgtatctatt
     6841 acgggacaga aatagggatg actggtgaag atgacccgga ttgccgcaaa tgtatgatat
     6901 gggagaagga ggaacaagat ctagagctat tcggttttat aaaagaactt gtttctctaa
     6961 ggaaacagtt gtctaagatt atttctgaag gttctactca gtggctaata gtaaatgatc
     7021 gtgaagataa gctctatttc acacggaaac tagagggaca gatcatctat gtttacttta
     7081 atcagtcaaa agaaccttgg gtagtagaac aggaaaatga ggtgattctt agtcaaaatt
     7141 gtcagttgtt agaagacggg aaagcagaga tacgacaata cggatttttg atcatgtgtt
     7201 gaaaactgtt accggtaaca acaagaaagc gtttgcaaat gaagatttgt gcccattata
     7261 ataagggtgt aacagcacgt tgaatacaaa ggaggaaatg aagatgaaaa aaagaagtgt
     7321 gaaacagttg ggtttagggt taatctcttt aggaatctta ggcggattag cggcttgtgg
     7381 caatggtaac tcttcagcag aaggggaagc cgatgaagga aaaacgttaa cagtttccgt
     7441 tgattctgga tataaagatt atataaacga aataaaaaac actttcgaaa aagaaaacga
     7501 tgtgaaaatc aagctagtag aaaaggacat gttcgatcag ttggaatcat tagcgttaga
     7561 tgggcctgct ggaaagggac ctgacgttat gatggcagca tatgaccgga tcggtgcgtt
     7621 aggacagcaa ggacatctgg cggaagtaaa actgggaaat gaagcagctt acgatgaaac
     7681 agacaaagcc caagtgactt atgatggaaa aatctacgga gaacctgcag taatcgagac
     7741 ccttgttttg tactataaca aagatttagt cgatacagca ccatcaacgt ttaaagattt
     7801 ggagaatcta tccaaagaca gtcgttttgc ttttgagtca gaagcaggaa aaaatacagg
     7861 atttttagct aaatggactg acttttatta ttcctatggt ttaattgcag gttacggtgg
     7921 ctatgtattt ggtgatgatg gcacaaatcc ttcagatatc ggtttgaata atgcaggtgc
     7981 tgtagaagga atctcttatg caacagattg gttcaaaaat gtttggccaa agggaatgca
     8041 agacataaaa agtgcaggag acttcgccag ccagcaattc atgtccaata aaactgcagc
     8101 aatcattgac ggtccatggc aagcgcaaac atacaaagaa aacaatatca attatggtgt
     8161 agccaaaatt cctactctaa ataatggaca gccttatcaa ccgtttggcg gaggaaaagg
     8221 atgggtagtc agcaattatg ctaaaaacaa agacctctct caaaaatggc ttgattatgt
     8281 aacgaatcag gaaaatcaag aaaaattctt tgaaatggta aatgaaatcc ctgcaaacca
     8341 acaagctagg gaaacagcaa aggctaaaaa cgatgaatta acaacagctg tgattgaaca
     8401 atatgaaaca gctcaagcga tgccaaacat cccagaaatg ggcgaagttt ggacaggtgc
     8461 agaaaacttg atgttcgatg cagcatcagg ttcaaaaaca ccaaaagaat ccgcagatga
     8521 agcagtaaaa acaatcagtg aagccataga acaaaaatac tgattaaaat gataaccgtc
     8581 catttttata aaaaatggaa caggtgcttc agataaaagt ggcaagagcg agatggaaaa
     8641 ttatcttgct cttgttgcca ttatcttaaa agcagtttgc ggtttagcaa gtttagaggg
     8701 ggcattcaga tgcagcaaga taaaacaagc aatatcaacg aaaaaaaaat cacgctttgt
     8761 tcacttattc cagggatggg gcagtttgta aatggccaag cttttaaagg tatcgttttt
     8821 ttcgccatat tcgtcctatt cattggtcag atgcttctgg gcggatttca tgcattggta
     8881 ggattggtta ctttgggaag tataccaatg gaagatcatt cattatttat tttgattgaa
     8941 ggcactttgc aattcatcat tacaatcatt ttcttgattt tttacggttt gaatctttta
     9001 gacgcttatc gagtagcgaa gatgcgtcaa gagggcaaaa aaatcaatcg gacgatgaag
     9061 gaagtcatac ataatatagg tgatgaaggt tttccttatc tattgacact tcccgcttat
     9121 cttctcatga tttttacgat cattttccca gtcctagtta cattgttcac tgcatttact
     9181 aattatgact tcaatcatat tccaccagcc agtttaattg attgggtagg ggctaaaaac
     9241 ttttttagta tcttcttttt aagctcatat cgtaatactt tttttgcagt cttttcgtgg
     9301 accgtgattt ggactttatg tgcaacaact ttgcaaatca cccttggtat tctcacagcg
     9361 gttgttgctc accagaaatt tattaaattc aaacgtattt tcggtatcat tttcttatta
     9421 ccatgggcgg tcccagcttt tattactatc atgagttttt cgaatatatt caacgatagc
     9481 gtaggtgcca tcaatactca agtgattcct ttcataaacc agcttccttt tgtagatatc
     9541 agtgcaatct cttggaaaac ggacccgaac tggacgaaag tagcaattat cttagtgcag
     9601 ggctggttag gatttcccta tatctatgtg atggttacag gaatcttaca atcgatttca
     9661 gaagacttat atgaagcagc aaaaatcgac ggagcaaatg catggcagcg cttttcaaac
     9721 atcacgcttc ctgctgtctt cctagtggca gcgccaacat ttattacaca gtatacgggg
     9781 aatttcaata atttctccat gatctatctg tttaacaatg gcggtcccgg aagcgtaggc
     9841 ggaggagctg gttctactga tattcttatt tcctggatct ataaacttac gactggaact
     9901 tctccgcagt attctatggc ttcagcaatc acgttgatta tctccgtcat cgttatctgt
     9961 gtgtcattgc tggtatttaa aaagacaaat gcgttcaata tgggggatta acaatgaaaa
    10021 aatttcattc gagtgctcga ttccaaaaac gactttctca cttttttact tacttttttc
    10081 ttttaatact gtcagtcatt atcatctatc cgcttttgat cacagcaagt actgccttca
    10141 agtcaggaaa tattgcagct tttagtttag atctttcaag tgactggact ttaggaaact
    10201 ttactcggct attcagtgaa acactttacg ggacttggta taaaaataca ttgatcattt
    10261 ctgtcctaac aatgatcgta caagtgactg tagtaacttt ggcgggctat acttatagtc
    10321 gctatcgttt tatcggccgc aaatcaagtt tgatgttctt tttagtgatt cagatggtac
    10381 cgactatggc agcattgact gccttttatg tcatggcttt cttattagaa gcgctcgata
    10441 agtactggtt cttgacaatg atctatatag gcggtggaat tccaatgaat acttggctga
    10501 tgaaaggata ctttgacact gtaccaatag atttagatga atctgctaaa cttgatgggg
    10561 cagggcattt caggattttt ttccagattg tattaccgct tgtcaaaccg atgctcgccg
    10621 ttcaagcatt atgggctttt atgggacctt ttggggattt catgttagct aagttcctgc
    10681 ttcgaacacc tgaaaatcag actgttgctg tcggcttaca aacatttatt tctgatgtga
    10741 caaaccaaag agtctctctt tttgctgctg gggcaatttt gattgcacta ccgatttgtc
    10801 tattattctt ctttttacaa aagaactttg ttaccggcct gacatctggc ggaacaaaag
    10861 gataggagca gagtatcatg caaacatttc cgttaaacta tttttccgca ttacgtacgc
    10921 caactcaagt gtttgcggga cgcaaactat tatcatggcc taaatttttc ttgatatttg
    10981 tatttttagt ttccttgatg gtcatgcctg tgactctttt ttatgccaat cagatccaag
    11041 cgattccatt agaacaattt ctatccgttc attcgctgat cgatgaacaa ggaacccaaa
    11101 aattttctga acttgaatta tctgaaactg ggttacaagc atcgcaacaa acgatagctg
    11161 tgacccctga aattttggta ggtgtgtctt tatctgagaa acaacaatcc gatcatggaa
    11221 cttttattga ttttgagaag gaacaatggg tgatccaaca aaaggataaa tctggtatac
    11281 gccgctatac gatgaattat agcccatctt tccaaccaga ttctgtacgt acgcccgaag
    11341 atttccaacg atttttagaa agagaatttt atgccagtaa tcgtccaaca atcattttat
    11401 cttatagttt aagtcttggt cttgtcctat ttgtcatgac gagtctcatt ttattcgggg
    11461 cttccttttt cttgtggatg acaagaaaaa gccaactttc atccattcat acattcaaag
    11521 agagtgcaaa tttgatgctg aatgttatgg gaataggttc catggcagca gcagttgtag
    11581 ggtttatcca ctttgacttt atcttgatgc taagtgtcca aacaacgata actgtcttgc
    11641 tgcttttatg ggtttttgtc aaaacaagat tcaaagataa gcgtgtcgaa tgacaagttt
    11701 aagcgattta tgcgacaatg aatgtgaaaa aaatgaaaaa taaagtagtt tttgaaaaat
    11761 ttctgaaaaa attaaacttc ttagttgatt cttttctttt ttagtattaa cgtaataggg
    11821 tagttttttt aaggaggaaa atcatgttag agaaaacttt atacaatcaa ttattttcac
    11881 gttctttctc gctccctgtc gaagtaaatt attgggacgg tacaacgaaa caatacggag
    11941 atacggacac tcctccccaa atcaaaatca atatccatga agaaatccca atgaaagaga
    12001 ttacaagtaa cgcatctttg gcactaggag aagcctatat ggatcatcga atcgagatcg
    12061 agggaagtat ccaagcattg atcaacgatg cttatcagca agcaaacagc tttttacgag
    12121 gaaaagatta tctaaaatgg ctgccaaaga aagaaaaaca tacgaaacaa aaaaacaaag
    12181 aagatatcca tgcgcattat gacttaggta acgactttta taaactttgg ctagatccaa
    12241 ctttgactta ttcttgtgct tatttcaata cacctgaaga tacgttggaa caagcacaaa
    12301 tcaataaagt ccatcatatc ttagacaaac tctttatcaa agaaggcgaa acgttgcttg
    12361 atatcggttg tggctgggga acattgatgt tcactgctac caaagagtat aatgtcaagg
    12421 caactgggat cacactaagt gaagaacaat acgatttgat taccaaaaga ataaaagaag
    12481 aacatttaga agataaatgc cgtgtattat taatggatta ccgcgaacta aaaggcgaaa
    12541 cgtttgatca tatcacaagt gtaggtatgt tcgaacatgt aggttctgaa aatcttgaag
    12601 gttatttcaa agtggtaaaa gatttattga aaccaaaagg aacagctttg atccatggaa
    12661 tcagccgaca acaaggcggt gcgacaaatg cttggatcaa taaatatatt ttccctggcg
    12721 gttatgtccc tggtgttact gaattagttg ataatattac aaaaaacgat ttgcaactga
    12781 ttgatttgga aagcttacgt cgtgactacc aattgacatt agaacattgg acaaagaatt
    12841 ttcatcaagt ccaagatcaa gtcacgaaag aaatgggtga ttccttctat cgaatgtggg
    12901 atctatattt acaagcttgt gcagcttcat ttgaatcaag taatatcgat gtgattcaat
    12961 atttgcttgt tcaacctgga aacaatgaca tcccaatgca tcgtgcataa ttgaggcaaa
    13021 agtactgttt aaagactatt taaaaagaag ctaagtttta tgatgctcca gaagtgaact
    13081 tttttgtcta acttctagga ttgtctctaa agcttagttt ctttttcttt atattttcat
    13141 aataaaagtt tggttcttta atttttaaac atgacagaaa attttttctt cgttatactt
    13201 gtaatagaat actattttat aacaaagggg atcgaatcaa tgaaaaaaac tgttatttat
    13261 actagtttat ttgcagcttt gtttttagct gcttgtcaat caaataccaa tcaaggcaca
    13321 tctgggaata cgtcttccag tagcgaacag cgctcttcac aggtatcgga ctcctcaaca
    13381 aaagcaagca catcttcgtc tagccagaca aaaagttcta gtgaagcaag tactcagtca
    13441 gatatcaaag tgtctgcagc tcgtgcgatc gaattgttcc aagataaata cccagatact
    13501 gcaattacta gtttggaatt agatagtgat tggggatctt acttctacaa gatagaaggt
    13561 gtcgatgatc aaaaagaata cgaagcaaga attgatgctc aaaatgaaaa aatggagaca
    13621 gagaatccag aaactctaga tcgagatgaa caaaatggtc aaaagaaaaa agaagatggt
    13681 ctggatgttg ataaattgat cacgattgag gaagcagcaa aaatcgccgt cgacaaagta
    13741 ggaagcggta cggcaacaga ttgggatttg gataaagatt taggtactac ttattgggaa
    13801 gtcaaagtaa aagacggcaa tcaaagtaca tcagtcaaga tcaatagcca aacaggcgaa
    13861 gtactgtcaa ctgaacaaga tgattaagaa tttcttcgga aaacaagagt tgatttttct
    13921 tttagtcatg atacactaga caaaataact tgtttgaagg agcgatttac ttgagtttag
    13981 aaacgacctt tgccaatcat atctttgcca atcctttgat gaatgcatct ggtgtccact
    14041 gcatgactac ccaggaatta gatgaactgg ctcattcaga agcaggagca tttatcacta
    14101 agagctgtac gatcaatgaa cgaaaaggaa atcccgaacc acgctatttt gatgttcctt
    14161 tagggagtat caattctatg ggacttccaa acttaggttt ttcttattat ctagagtatg
    14221 ctcttgcgta tgaaaaagtg caggaaaacc aaaatcaacc gttatttttt tccattgccg
    14281 ggatgagtgt ccaggaaaac ttggaaatgc ttgagaaaat agaaaaaagt gggtttaatg
    14341 ggatcactga attaaattta tcttgtccca atgtgcctgg aaaaccacaa ctagcttacg
    14401 attttgaggc cacttatgaa acactgaaag aagtattcag catcttttct aagccgctag
    14461 gaatcaaatt gcctccttat tttgattttg ctcattttga tcagatggct gatatcttga
    14521 atcagtttcc attgacctat gtcaatgcta tcaacagtgt gggcaatggt ttgtatatcg
    14581 atacagaaca agaagcagta gtcatcaaac caaaagaagg gttcggcggc attggcggag
    14641 agtatatcaa gccgactgct ttggcgaatg ttcgcgcttt ttatacacgt ttgaaaccag
    14701 aaatccaaat cattggtaca ggtgggattc ggacaggaca agatgcattt gaacatctgc
    14761 tatgtggcgc aagcatgtta cagattggta ccgagcttca taaagaaggg ccggaaattt
    14821 tttctagaat cataaaagaa ttaacgcaga ttatgtcaga aaaaggatat acgtcaattg
    14881 acgaatttaa aggcaaatta cgcaccattt catagcaaag agacgaaggt catgattgat
    14941 cttcgtcttt ttttataaaa aacactcgtc tgttcattcc tgtgcaagcg tactcattgt
    15001 atgttagacg aacttgtggg aaaataacag aggcaaaaag gaggataata tgaatgatgc
    15061 aacagaatta gcaaaagagc ttaaagaaaa agaatttagt tttcaagaac tagttacgga
    15121 tatacagaaa aaaataaaga agcaaaatca tgaattgaat gcgtttgtcg cgcttgaacc
    15181 attcgagagt aattcccacg agctattgat tccccagtca gaaaaagggc cattggctgg
    15241 tatcccattt cctttaaaaa tgctcggtca agaaaaaaag ggatggctag ctacttcagg
    15301 atctcgttta tttgaaacac accgagcttc tcatacatct aattatgtgc ggcaagcaga
    15361 agcgataggt ctagttcctt ttggacaaac caatgcgcca gagtttggtt tcaaaaatat
    15421 tacagaccca gtcatctatg gcccagcaag aaacccttgg aaccttgaat gtacaccggg
    15481 cggatcaagt ggaggagctg ctgctgcagt ttcatcagga atcgttcctt tagctggtgc
    15541 tagtgatggc ggtggttcta ttagaatacc cgcttcgttt tgcggattga ttgggctgaa
    15601 accttcgcga gggacgatgc cagttggtcc atatgcttgg agaggatggc aaggagctgc
    15661 tattgatttc ggcttgacag tttctatgag agacacggag gctttatttt atgggatgag
    15721 aagtatccat tccggtgcgc catatcaagt atcgccagtt gagtggcaaa cacatccaag
    15781 aaaaaaacgg ttgaagatag ctttttgtgt tgattcaccg atccactcac agattagtga
    15841 agaggcagta cttgctgtaa aaaaagctgt agatttcctt ggaaaagaag ggcatgattt
    15901 gacagagatt ccttacccac tagatggtcg taaactgatc acgtcttatt atcagatgaa
    15961 tggcgcagaa acagcagcaa tgatccgctc gattgaaaca ggcatccagc gagctgtcag
    16021 aaaagaagaa cttgaaccca tttcttggac tttgttccaa tatggtcaga agctctctgc
    16081 cgctacttac atcgattctc tgcatgtatg ggatgaagca gcaattgtca tggaaaacct
    16141 acatcaagaa tacgatctat tcctttcacc aacgactgca tttaccgccc ctaaaatcag
    16201 cgaagatttg caaagtgatg cgataagagc acagatggaa cacatcagtg attgttctga
    16261 agtagaaggt ttagacgttg tttcttccat gtttgaggag agcttgaaga tcacacctta
    16321 tacacagttg gctaatctga ctggacaacc agcaatcagc ttgcctactc atttgacaaa
    16381 agaaaacatg cctctaggaa tccaatttat ggctgccaga ggaagagaag atttgctatt
    16441 tcaaatcggc tatctatttg aagcaaatca tcagttccat ttaccaaatc catggaaata
    16501 gcagaaacaa ggagaagaag aatgaagaaa aaaagattgt ttgctctaat aactagtgcc
    16561 ttttttattt taagcgcttg cggtacagct gccgctaatc aggaagataa cagctggagt
    16621 aatgtagaaa aaaagaaaga attagtagta gcgacttctg gcacactgtt tcccgcatct
    16681 tattataatg atcaaaatga attagttggt tatgatgtag atttggcaaa agaggtggct
    16741 aaacggttaa atgtcaaaat ctcctttaaa gaatacaacg tggatggaca aatcacttct
    16801 gtttcaaaaa gagaatcaga ttttgctgcg aacgattttg gtatcacaaa agaaagagaa
    16861 aaaaaattct ccctttcaga gactttcaaa tattcatttg acagtatgat cgtacgaaaa
    16921 aaagatgatt caggaatcca ctcacttgaa gatcttaaag ggaaaaaagc agctggcgaa
    16981 cccaatacta gctatatgcg tttagcggaa aaacacggag ctaaattagt cacatatgac
    17041 aatgccacga atgatcagta tttaagcgat gtagcaaatg gtcgaacaga tgttattcta
    17101 aatgattatt atctgcagaa aatggccgta tctgctttac cagatatccc tgtaaaaata
    17161 ctggagaacg tttatttcaa tgaaaacgaa agtggttttt tattgaaaga aggaaatgat
    17221 gctctgaaaa agaaaatcga tggcgtatta aaagagatga aagaagatgg gacgacaaag
    17281 aaaatatctg aaaaatattt tcaaactgat gtttcagtca aaccgaaaga aacgatggct
    17341 gagtcggtga gtgagtgatg tactttcctc taattgattt ttccctcatg tggacttcac
    17401 ttcctttcgt actgcaaggc ttggtataca cattagggat tggcttcgtg tcgtttcttt
    17461 taggaaatct ggtagggttg cttttgacag ttttaggtct tttggattgg ctgcctctga
    17521 atgtatttat tcgtttttat ttatcgttct ttagaggcat tcccgcacta gtcctgctgt
    17581 ttctgttata ttttgggtta ccttatcaat taagtgcctt aacggcttca gtcatctgct
    17641 ttacgattac cagcagtgca tttattggtg aaatctatcg aggatcacta gcaggcgttt
    17701 cttcaggtca gtgggacgct ggtttagctt ctggtttttc tttctttggg gtattgaaat
    17761 atatcgtttt gccgcaagct tttcgaatct cagtaccggc actcagtaac gtagctatgg
    17821 atctagtgaa aggtacttcg ttagcagcaa caatcacagt gccagaaata tttcaaaaag
    17881 caaagatcat aggcggtcgg acattcgatt atttttctct ctatatttta gttgcactga
    17941 tttattgggt catttgtatc gggatcgaaa aagtgcaaca gtatttagaa aaacgttcta
    18001 tgcatcagtc aatctgaaaa atagaaatgg tgctagcgtg gctggcacca tttctatttt
    18061 ttgtttcttg tatattctgc ctagtatttt aaaaccattt catgtaaaat gtaaatcgaa
    18121 aatatgttcg gaggtagaaa acatgaagac cccgctaacc atcgaaatag aatcctcgtt
    18181 ataccaatat tgcatcgagc aaggcggctt ggttgtagaa gaagtcacga tgcctgatga
    18241 ccaaggaatt gtagatacgc ttagctgctt gacaaaattt gatggaagcc gagaatggcg
    18301 atgctatgag ctgaaagtga caaaagcaga ttttcgctct acagccaaac tgtcatttgt
    18361 tggacattat aattattttg ttttaccaag taaactatat gaagaagtaa aaactgaaat
    18421 ccctacagga ataggtgttt tagtgtatcg tccgtacacg gttgaagagg agatgcctgc
    18481 ctctggtaca tttctgattg cgaaaaaagc agcaaagaaa gagcttgctg taccggaaga
    18541 agctttgaca aaccgcttca tggcttcttt attcagggaa gtaagaaaag caaagcaaat
    18601 ggagcgaggc gtaaaatatt ttccaacaga tcaattgtac aaagaattga aaagacgttc
    18661 agaaaatcaa gatccatttt ctaacgaacg atattatgaa cggtttttag aggaaatagc
    18721 tagtcaggca atcaatgagt tgcaagaaga agttatcgcg cttcagcatg attatgaata
    18781 tttgaggcaa caacagcaga tccgtcggtt gcctacagag ccattggagt gaaaaatatg
    18841 tactatcctt atttaagagg aaaacaattt gacctattag cattaaaaga agcactgtcg
    18901 cgaggattgc tctccaataa aatccaacca gtgatcgaac cagtacgtga ttccgctacg
    18961 cttaaaaatg tgatcgaatt atttcaaaaa aagcaccatc gaatcgctgt gatccaaaat
    19021 ccccaagtag gacaattcaa attattcgat cagcatgtgc acagttggac agtcaaagaa
    19081 aatagctctg tcgtccctgc acagatctta acgccagaaa atctcagtga agtactggat
    19141 tcacctccag cgtttcttgt tttcgatgga cagcactacc caaaagatac agaggtgtgg
    19201 aaacagttgg caggagttga ttcgaaattt ttgataccag atactagtcg ctttcgcatc
    19261 tggctgcccg aaaacaaaat tgtgatccgt gactcttttc aaacgagaaa gcatgttgaa
    19321 agttatgcag ataaaacaga tgactttttt agtgatgact atcttttttt ccacgcagac
    19381 ggatatattg gattttctga tttcacgatc gaaggtagcc gttatttcga taaagggttc
    19441 cctagccgag cgcttgctat ccatctaacg tatattgatg cttacggcaa tatacgcgtg
    19501 aaacattttg tctctgacag taacgactct gctaaagacc aagcattgaa gtttcttgaa
    19561 gcaggagata aaatgaaaaa atggatcatg cgtcaccatc atcaactctt gattacttca
    19621 ggaatgattg aattattggc tctttaccag caacaaaaat tccctggact gggagtgttg
    19681 aaaaagtggt cacttatgca tcatttggaa cttattagtc acctgctaga tcatccaaaa
    19741 gattggctta aatattattc aaaagttcag gaactatatg aagtgatcca atgattagat
    19801 aatgtaaatg aaaaaggaat agaaaagagg aaactggatg tatattcggc aaacaaggaa
    19861 agaagaagta gaagaagtgg tagaaatcat taatgccgca gcggatttca ttgcttcgca
    19921 aggctctcct caatggcaaa acgggcagaa accaacaaaa gaaacgattt tgaacgatat
    19981 tgaaagaaaa gaaagctatg tattaatagt ggaagatcaa attgctggaa cagctgcatt
    20041 agtctcaagc gtggatcccg tttacacaaa aatagatggt agttggaaag aatcaactgg
    20101 tccttattta tcgattcatc gtgtcgcttt agcagaaagt actcgaggaa aaaaactagc
    20161 aaaaccgttc ttagaacatt tgttgacgat tgcagccagc aaaaatatcc aagatatccg
    20221 tatcgatact tatccagcta atgaaccgat ggaaaagacc atctattctg ctggattcac
    20281 ttatcaaggg atgattgaat ttccttttga acacggcgaa agaaaagcat atcaaataat
    20341 catcaattga ctataaaatc atctgagcaa agataaagac attaacacta ctgaaaaaaa
    20401 caatcatatg atagccaaga ggttgtaaca gaagtggcca gcttcaaaaa acaagaaaga
    20461 atttccgaaa atgcttctca aatttttgtg gaattttagc gtattttacg aaatagctgc
    20521 tttctgaaac tcccttcatc gagaggtcgt gacatagctt ttgtcacgac ctctcggctt
    20581 atcctacgag tattattcga tacatttata tcaataatcc tttttttatt ttatctgcag
    20641 cttcttggaa cgctccatcg tttgacacga ctaaaagtga ggggatagct ttcaaacgtt
    20701 tcaactgtgc taaatcttct acataaaccg ccagtctggc tgaaatctgt tcagatgaat
    20761 ctccacgttt tttcaggcgc gattgcaatg tttctttaga gatcgtaata aagataggac
    20821 ggactaccgt cccgaaatac tcatataaat gccaaaaacc atttgctgtc aaaacggcgt
    20881 aacaatttcc tactgtcagt ttctttttaa tttctgcttt actggaacca tatttatgtt
    20941 gagcatattg gtcccactct gcaaactcat ttcccttgat catctgattg aatgtattgt
    21001 cagaaacaaa ataataatcc tcttgatctt tttccccagg tcttggtgat cgattcgtgt
    21061 aagtaatgat cttttgttcc ggctgaaaaa tagcttctgc gagtgaagtt tttccagaac
    21121 cagaaggacc gataaataca tagcaataat tcattgtcat ttctcctttg atgattgaac
    21181 aagtgaaaaa gctttggaaa atatttccaa agcttttaaa agtaactttg cttttttgta
    21241 tattatacca gcaagccagt cacaatagca cttaaaaagc tgactaaagt cgctgttagc
    21301 aaaattttca atccgtgttt tgcgacaaga tttccttttt cttcatttaa tcccttcatc
    21361 gcaccagaaa tgatcccgat agaagagaag ttggcaaatg aaacgaggaa gacagatata
    21421 attgctgttg tacgctcagt aaaatggaaa ttaccatttg ctaattctgt catagcaaca
    21481 aattcatttg taaccagttt agtcgccatg atactaccgg catccactgc ttctttccat
    21541 gggattccgc tgatgaaggc aagaggggca aagatgtaac ctaggatctc ttggaaagtg
    21601 atgccgaata tcccgtggaa gatagcattg atcatggcaa tcaaagcaac aaaaccgata
    21661 agcatagcag caaccgtgat cgccacatgg aaaccatcaa gaatgtattc tcccaacatt
    21721 tggaaaaatg tctgttcttt gttttcttca atgatcagct catcttcttt ttcttctaat
    21781 acatagggat ttacaattga agccagaata taaccgccaa ataagttcaa aacaagggca
    21841 gtgatcacat atttagaatc gatcagtgcc atataagatc cgacgatcga catagaaact
    21901 gtagacatag ccgatacact catggtgaac aaacgtttct cagataggaa gggtaattcc
    21961 ttttttatgg aaataaaaac ttccgattgt cctaaaatag cagaagctac tccattataa
    22021 gattccaatt tgcccatccc gttgatttta cttaatccta gaccaacagc tttcataaat
    22081 aatggaagaa tcttgatata gcgtagaata ccgataatag cagaaataaa tacaattggc
    22141 atcaatacgg aaagaaagaa aggtgcactt cctttatttg ctacaccggc aaaaacaaag
    22201 tcaacacctt gcccagcaaa agcaagaaga tgatcaaaca tttttgccaa tacagaaaca
    22261 accgctgtac caaaagttgt acgtaataat acgaaaccaa gaacaagctg taaaacgaac
    22321 atcacaatta gtggtttcaa tttgattgct tttttgttac tgctgatcaa aaaagcaagc
    22381 cctaatgtga caaataaacc aataatacct aataaataac tcaaataatc actcctattc
    22441 caaattccgt tttattttac ctgttttgca aataaaatac taatctttag tgaaattttc
    22501 attacttttt tagagtgaaa cgcattcatt ttccattaga aaataaagcg aacaaaataa
    22561 aaggtttaaa aagtgtaacg tcttagtaaa gtctataaaa aaacgaaact atcgattaag
    22621 ctaatagttt cgttttcaac atttataagt aaactttttc tattggtaat atagattttc
    22681 ggaagggtag acaggtgcgc aggtaatatc gattcctagt tttttcaatg tttgttcatc
    22741 atttttattg accatcacag tagagtgggc ttgtgctccc tcaagttcag ctagtttatc
    22801 ataagcaact tgagcgattg gattcgttac tgcactgatt gataaagcga tcaagacttc
    22861 attgatgctt agtgcatcca aagaggaatg aagtgtcttt gatttcaatc cttgtattgt
    22921 ttcaagaact aatggtgaca ataaaggaat gtcatctgcg atatgggcta aatgttttac
    22981 tgcatttaag ataactgccg ctgacgcatc catcaatgaa cttgtacgcc cggtaatgat
    23041 gacttcatct ggtaattcga ttgcaatcac agcagaagga tcttttgtag gactttgcgc
    23101 ttgaatctta tctgcatagt catgggcagg gactaccacg tttcggtctt caggacgtaa
    23161 accgacttct tccataatca gtttgattcg attaactgct tcttcgtcga tcaatccttt
    23221 tttgaagtcg cattctgttt ggaaataacg acggatgatt tcttgtttag aggcttcttg
    23281 gacaacggta tcatccacga tcccaaatcc tacacgattc acacccatgt cagtaggaga
    23341 ttgataaaca gattctttac cagtaatttt ttcaataata cgtttgatta caggaaatgt
    23401 ctcaacatcc cgattatagt tcacagctac tttattgtag gcatcaaagt ggaaggaatc
    23461 gatcatattg acatctttta gatcgactgt ggctgcttca taagcaatgt tcagcggatg
    23521 tttcaacgga acattccata ctgggaaagt ttcgaacttg gagtaaccag ctgcatgtcc
    23581 tttttggctt tcatgataca gttggttcaa ggaagtagcg agctttccac ttcctgggcc
    23641 aggagctgtc actacaacga ttggtttagt tgtttcaata tactcatttt ttccaaaacc
    23701 atcttctgag acgatttttt ctacgttgac tggatagtct tcgatttctg agtgtttata
    23761 gaccttgatg tttcgtcttt ctagtttatt gataaagact ttcgtggcag gctgtccact
    23821 atagcgagtg ataacgacac tgttgattaa taagccataa ccacgtaatt catcgattaa
    23881 ccgcaagata tccatatcat aagtaatccc ataatcacca cggatcttat tacgttcaat
    23941 atctccagca tagacgcaaa tcagtatttc agcttgatct ttcaattttt gcagcaattt
    24001 gattttcgca tcttcttcaa atcctggaag tacacgtttc gcatgcttgt cgtcaactag
    24061 cttcccacca aattctaaat ataatttgtc atagtggttc actcgttcca aaatatacgc
    24121 tgactgctct tcaatatatt tttctgaatc aaaaccaatt tttttcacta ggttccctcc
    24181 ttaagtacta aagcattttt tcattttttt gaggattcgt caactggaag agcgaaattc
    24241 ctcaaaaaaa caaagaaaat ggtttaaacc aacttgtcta gatagttatt cattggtatg
    24301 atagtttttg ataaggaagg tgctacatga aaaaaagaca gaaaaagaaa aacgcatata
    24361 aacactatat ccgttcgatt tttaccggtt atgaaagaat gcttgaagat cccgaattgg
    24421 aacaattgac gtttacttat ctaaacgaag aaacgcagct aactcgtgat gagcatcagc
    24481 gcattcattt cacaacaaga gatctgccta gtaaataaaa aataagataa aggtcatcgg
    24541 attcgattct gtcctatcag atggaaagac tagttgatcg aaaaaaaggc tgtaccaaat
    24601 tttggtgcag cctgttttat ggagcattgc ccgctctttt ttataacaat tttttacaga
    24661 aaatggtact attgaagtat taataagtga aaaacagatg agtaagagga ggcaagtagt
    24721 atgaaagagc cagatacaaa tgcaaattat gagccagacg aactagagga gacgaatgcg
    24781 gatcaggata caggacaaga atcgtcttct ttttacgata tcaaagaaaa agcaagcatg
    24841 aatctttccc aattattaga agacttacag gcttttgaaa aagctgtccg cgaagaagat
    24901 atccctgaga tctaccgttt atacaatggc cgattgaatg aagaattaaa aagaagctca
    24961 aatgaaaatc acgagatcga tcaattgctg atgcgtaaga tccatgatcg ttttttacag
    25021 gaatttcctt ttatggaaca agtagaaaac atctcgccaa cgatgagcta ttacaaaatc
    25081 ggcacttatt accatgatcg gcccacggtc ggtattgatg caagtctgcc agaaattttt
    25141 gttttgccta agatcgatga agagtgggag aaatattccc aaccggagtc aacggactat
    25201 gacaaaaaaa tcaatgagct agacgcaaag gtgatcactg ctcagacaga aattgaacgt
    25261 ttagatgaac aaataaaaga aatcaatcga caaatgactg atttgaacga caataaagga
    25321 ttcttgaatc gtaaaaaaat cgaggaagaa attcaagaat tagagaagaa gaagcaagta
    25381 ctatctaatg aaaaattagg ctggcttcct tatattgaga cgcctgaaac aatccagcag
    25441 caaaaagaag cattgaagca agaagcaaga gcagaccagc ttcgtgcagc catcgtagaa
    25501 aaagaacaaa ggcaaatcag acgctatttc ggtagcaaag agggattcgg tcaagcaatc
    25561 catgaattct tgatgaatta tcttggcaat gagaatccta ataatcaatc taatgaagga
    25621 ggttccgagt atgaatgaac acgatgaagc agaatggaca aaaaatgaag aacaaacaga
    25681 agatcatgaa gaaacgaaca cttcattgga agaacaaaca agtaatcctc tggatgacac
    25741 agaagaaatc cctatgctag aggaaacaaa aatacacgaa aaagaaaaag taactccgac
    25801 gaatacgaat actgcagaaa acacccatgc ttacgaaata actgaaaatc ccgggtattt
    25861 agaaggaaaa gagcaagatc atcaagatat cgtcagtttg attgaaacag cggaagcaga
    25921 attagcggat ttacgtatga gaaaagattt gatcgaatca gattttccac aattattaga
    25981 atcatatgat cgttttatat tgaatgaaga accaatctcc acgatcggaa agacaagcct
    26041 gatcgaatac ttcacgtatg aattactgaa accgttgaat gatattggtc tggaacggtc
    26101 aaattcagct tatgacacat ggaaaacaag acatttttcg atcaattata cgttgaatgg
    26161 acaaaacgaa ttagtctttt cctttgtatt accgaccatc gatcaacgat accaagtgga
    26221 atcgatgagt ctcctaaacg tatcaccaga gacaatggaa atcaatgtcc aagatgaccg
    26281 ggtgctttct ttgatccgac actggtcagt ggatcgagta ttctctgcag ggcagatcac
    26341 gatattcaat cacaaattga atcaattatt agcccatgca agaacacttg gttttaccgt
    26401 taatcagaca ttactagaca atacgaagcc acttcgtttg aatcttcaga gcgaatttga
    26461 attgacgaat gaagtattgg atgatatttt tatcgtggca atgaaaaatc catcctacga
    26521 tatggaaaag atcaacgaaa ctacttatca agtacgatta gataaaggac agtctttaac
    26581 gataacaaca aacgaaaaag atcaaacagt cttgtcgatc tcatctggag actacccacg
    26641 atctgtcatt gatttcttta tcaattacga attcttggtc ccattaatgg tgagaaagtc
    26701 ctgattggat aagttggatt accagatgtc attcagataa ttctgttcca aaggacttgc
    26761 ggcagttatc cagaataatt gtcacaagtc ttttctgtat gtactaaatg gacgtgaata
    26821 acttgctgat gaaggaggga tttcattagg aatgtccaca aataaaatag cacagcttag
    26881 cttattgtct gctgcttgtg tagcaggtag gatcgctttt caatttattc cgaatttcca
    26941 gccaatgact gctatttttt tatttatagt actttactta agcttaaaag atgcgttaat
    27001 tgtcatgagt ctttctattg cgatcagcag tttttatttt ggtgttggac catgggtcat
    27061 cggccaatgg atcagctata ctgttgttct gagtttgttt tcaatcgtct gcttgagaaa
    27121 aacagtgcaa aggaatctat ggttaaaggg agtctgtttt tttctggcgg gcatggttta
    27181 cggaataggt atgaccgttt ttgatacgct gttatatcgt ttgcctcaac cttggatcta
    27241 ttaccttcaa ggggtgtcgt ttgatttgat gcacagtata ggcaatgttg tgttctttct
    27301 tgtttttctg ccagtcgtca aacgttttta caatcattca ggaggaaaaa atgaaaaaaa
    27361 taatttatag tttgcttgca atgagtgtca gtatactcat tttgacaagt tgcagtacaa
    27421 cctatacaca atcggcttcc aaccaaaagg aggaagtaaa aacatcgcaa aaagcaacaa
    27481 tcacgttaca agaaaatggt caaaactttt cggaaaaaga agtgttcttc aaaaaaggcg
    27541 atgatttact gagtattttg aaacgaaatt ttgaagtcaa agaagataac ggcttcatca
    27601 cttcgcttga agggcactca caggatgaga aaaatcaact ctattggatg tttactgtgg
    27661 acgggaaaag tgctactact ggtgccaaag agatcaaatt gaatgatgga caatcggtca
    27721 ttttcaattt gtctaaacta taagtttcat aaaaaactgt taaagggcga atattgccat
    27781 aattcagtga gttcagttga tgaaagaagg aaaataacat ggaaaatttt aaaaaaagcg
    27841 gaatgttttc cgtaattgct gctctagggg ctaatattct agttgctatc agcaaatttg
    27901 tcggctatgc aatcagtggc agtgccgcca tgctaaacga aagcatccac agtatcgttg
    27961 attgcagcaa tcagattttg ctgttattcg gtgataaaca agccaataaa ggacagagcg
    28021 agttgcatca atttggtgaa ggacgagcaa aatacttttt cagtacaatc gtcgcaatga
    28081 tgcttttctt cggcggtggg gcacttgggg tgatggaagc ttttgaaaag ttgacgcatc
    28141 catcccatga agtgggtaat ccgatcattg ttatagcaat actgttattt ggtttggtaa
    28201 tcgaaggaag ttcgttacgc gtggcaatga aagaaatcaa agaattgaat actgaaaaac
    28261 tttccacgat gcgctttttg cgcgaaagtc gacacagtga aatactgatt atttttacgg
    28321 aagacttgtg tgctgtaatc ggtttggtct tggctttagc aggaacaata ttgacttatg
    28381 caaccgggat tgctgcattt gatgcgatca gcggattgct gatcggtttc ttgctgatgg
    28441 gagccgccat tttcctagca aaagaatttt atagtctgat catcggtgaa agcgtgacag
    28501 caagcgattt atcaaaaatc aagttagctt ttgaacgtcc tgagatcagt cggctgatcg
    28561 atataaaaac agttcattta agtccaacgg agattttagt tgcagcaaaa gcagatatcg
    28621 ttggtaacaa agagaatgaa gcttactcgg tcatcaatga tattgaaaaa aatatgcgtc
    28681 agtctcttcc tgacaagaag atgtatatat acattgaaac agataaatat gacccaaatt
    28741 attctcgtaa ataaaacgtt tattcatgaa ttgaagcaaa aatgagaaaa atataatctt
    28801 gtttttaaat aaaacggcta caatttagcg aatttatgtt acaatgagtc cgctgtttat
    28861 ttgttgtaat taaaggagat attttaatga acaaaaagaa aagtgcgcca tggcttcaag
    28921 ctttgcatgc agtgatgcca ctttgtataa gctatatacc agtaggatta gcttgcggag
    28981 tattgttgca aaaagttgga tttgatcctc tcttatcagg actgttatcg attctggtct
    29041 tttccggtgg cgcgcagttt ttagttgcat ctatgcttac gacgcaagca tcatttgcaa
    29101 cgacattatt gatggttttc tttttagaat tacgctatac attattagga gcaagtcttg
    29161 ccggttttat gaaaaaagaa aagcggacgt ttttggctgt cttttctcaa tcactgaatg
    29221 atgaaaacta tgcggtgaac tacttaaagt tttcaactga cccttcctgg aataaaagaa
    29281 aggctttata tgtcaattgg ttctcaatga ctgcatgggc cggtagcaat atgatcggta
    29341 attttttcgg ctcggtgatt cgagtagatg ctgaccttgt ccattttgct ttgacggcca
    29401 tgttcatttt catgtttatc atgcagatga aaagcgcact actgatcttt acaggtttat
    29461 tctcaggcgt attaggggtc gtattcatgg tattattcca aaatactttc ggattgattg
    29521 cagcaacagt cattgcttca tttgccggat ttatgctgga gaaggcattc aaaaaagaaa
    29581 aagctaagaa actaagagac cgcggtaaag atgtcaatga gccgctcttc aacttcccgg
    29641 aacaggaggc caatcaccat gactaaacta tatttgctac ttttagttgc ttgtctgttc
    29701 atcgtggcat atatcccgcg cttgttcccg atgctttatt ttactcatcg aaaagtgcca
    29761 tcctggttca gtgactggat gaaatatgta ccggtggcat tatttgcggc gttagctttt
    29821 aaagatgttt tcattactca tgaacatctg gatattgctt ggaatatcaa aatagctgca
    29881 atgattctag tagcaggcgt ggcttacaaa acacgttcga tggcgttgtc agttttgaca
    29941 ggacttgcgt ctgtcttttt gctgtctatg ctttaaactg ttttatttta ttaaaaacag
    30001 agattgcgac ataagttgtt caaactgatc tatccgaact gcatttgcaa cagtcaattc
    30061 tggatgcaag tttattcgta gggataaaaa agattagtgt tggcttatga tgcaagctct
    30121 tttttatttt aggaattaga gagggatttg tccttctatg attttttgct ataataggga
    30181 agatgtttat tttatgaaag aatagttgag ggaatttaaa tgaataaaaa attgatgcaa
    30241 ggaacatttt ggctaacctt tgctaatctt ctatgtaaag ttttaggggt agtctatttg
    30301 attccctggc taagtatgat ggggaacaat caagatggta tgcttgctac gacattgtat
    30361 aatgttggct atcttcctta tggattgttc ctgatgcttg gtacagtcgg atttccaaat
    30421 gcgattgcaa aaaaagtagc tgtagccaca aaagaaggag ataacgctgc ttgtcggctg
    30481 atttttagaa gtacgatcaa tatcatgttt gtcatcggta tcgcctctgc tgcactgatg
    30541 tatttatttg ctcctttact agccggaatc agtccgattg ctaacgttaa taatgggata
    30601 ctagctatca gaagtttatg cccatctttg attgctatcc caattcttag tgcaatgaga
    30661 ggatatttcc aaggaaaaaa tgatctgcgg ccttatggaa cttctttgat tatcgaacag
    30721 gctgtgcgag tgatcgttat cttggctggt acttattact tgcgcgtatt gactgacgga
    30781 accatattag aagctgtatt gatcagtaca gtcgcttctt tcttcggtgg gttagctgcg
    30841 attatccata tgtatatcgt cggacgcaga aaagacttct tcgaacttaa agattttttg
    30901 atttccagcc gttatttcga aagagaaaac cgatctgcct ctgtctctat cattcgggaa
    30961 acactaccgt ttatttttgt cggctcagtc attactatcg ttcaaatgat cgaccaagta
    31021 acgatgaaac cactgcttca ctttttccgt ccagagatcg cagatcagca actggaattc
    31081 ttattcagcc gtgcttctgt caatccaaat aaattgacat tgatcctgat ctccatggta
    31141 ggaacagtag ctataagcag cttgccaata ttaagcacga tgaagaaaaa agatcggata
    31201 caaatcgaaa aaacagtagg agatagtttt tcgattgcgt tattgattct tcttccttca
    31261 ttgactggaa tgtctttatt agctggacca ctatacacat tgttttttgg ctatgatcct
    31321 gaaagtgtcg gatatttcca aatggctttg ctagcttctt tgtttttctc tttattcacg
    31381 atcctatcta cgatgcttca atctttgaat caccatcgtt ttgcgatccg attaactgta
    31441 gaagcaatta tcctgaaagt catcttccaa gtaatcggat taggattagt tggcggatac
    31501 gggatgagct tatcaaatgg cttagcattc ggtattgtct ttgttcgcgg ctatcacttt
    31561 atgtgcaaag aataccgaat agcaccttta gcaaagatca gcaatttttt cttaaaaact
    31621 ttccgctcca ccctgattat gcttgcagta tgttttgccg ttttcttttt gctgaatcag
    31681 cgattgtcaa tggaatcaaa aagctatgca atgatttatt gtgtggctgt aggaagtatt
    31741 ggcggcttag tatttttatt ttctcaattc ggaaaaaatg ggtttcgaat gttgaaaaat
    31801 gttaaacatc atagcagaag caaagaatag gttaactaat cgttttttgc tgtcacttga
    31861 aaaacggcaa ctcgtatgat tacgcttgtc gtttttctat tttccaattt tagtttcata
    31921 tctaatctta cttatgatat agttataagg tctggaaaaa ttctttgaaa cgaggaaatt
    31981 aaacacatga aacttgcagt agttacagat agttcggcat atctgccgga acgtatcaag
    32041 aaccatcccg acttattcgt tattccaatc cctttgatta tggatgggaa aatttataac
    32101 gaaggaatcg atatcgaagc ggatgaatat tatggacttt taaaaaacag taaagatttt
    32161 cctactactt cacaacctgt cgtaggggaa gttttgggtt tatatgagga attgaaagag
    32221 aaagggtatg acacgatttt aagtatccat ctttcttcag ggatttctgg ctttgtcaac
    32281 acattatttg cgatgaaaga tgacatcagc ggagtaacgg tcattcctta tgactctaag
    32341 atcactagta tgccgatggg acacatggta gaagctgcct tagacttaaa tgatgaaggt
    32401 cagtcccttg atgaaatact tgcacatatc gatcgaatcc gagataatac ctacgcttat
    32461 ctgattgtag atgatttgaa caatttagta cgaggaggac gtttgacaaa tggtgcagcg
    32521 ctgattggca gcttattaaa aatcaaaccg atcctgacat tttctgaagg gaaaattgtt
    32581 ttattcgaaa aaatccgttc aagtaaaaaa gcatttgctc gagctgaaga aattattggt
    32641 agaagaaata acgaaataca accgccagtc aaactttacg tgatacatgc aaataattta
    32701 gaagtagcga ttgcggagaa agagaaattg cagaaaaaat atccaaatgc agaaatcgaa
    32761 attggtcatt tcggaccagt cattggtaca catttaggtg aaaaagccat cggcattgcc
    32821 atttcagcgc aataattcct tttattcaaa aaacatacca aaaatattcc aacatcatct
    32881 tggactttct acatgttact aatgagcgtg taaaagaacg agatggtgtt tttatttcta
    32941 tcaatgaagc tctttttctt tgcaatcctc acttcaatcc tctataatgt cgcatatagt
    33001 ttagtaaatg agtaaactac taatttactt atttactaaa aataaaagga tgaggagtta
    33061 aaataatgga aataccaaca aatttaaaac ataaaccagt aatcgttgta gaagactatg
    33121 ataaagttga tggaagaaat gcattaaata cggatgcaaa agggctttca ctgggccttg
    33181 ctcagtggaa tgatcgtgga aaagtcgata tctcaggcaa ggtatggcga catactggag
    33241 aaaaatggtc acgacagtcg gaagaattac caatcacacg tattttagat ttagcgatct
    33301 tagctgcaca aggaagttta tacttccaag atgcttatcg ttatgaaaaa ttttatgatc
    33361 cagaaaatcc agtcgtagac atcattggat tacaagggaa ccgaatgacg gtagaagtag
    33421 ataccaaaaa tccaaagatc gatgaagaca tcattttgta ttacgataca ctacaaaaag
    33481 acggagaact gattggggaa cgattccgta ttttaaaacg tcttttagat gagctaggat
    33541 attaatagac agagatgatc aaaaggggaa gagaagcatg gagaatttct cactaacaga
    33601 aaaccgaacc ttttcacggc tagaaaagca aatgatctgg aagaaaccga aaagccatcg
    33661 gacaagtgca gaagaatggc ggatcgcctc cgaaatcaaa gggaattggc aagatcctga
    33721 aatgaaaatc agtaacgtac ttttggaagg agatgccggg tcggggaaga cacaattagc
    33781 caaagcacta tctgctgatc tgcaacttcc ctatacaaaa gtgacctgtt ttgcagatat
    33841 ggacaaatca gatgttttcg gagccttatt acctgtagta gattctaata aggaacaaga
    33901 ccaagaatta ctagaagcta tttaccaaac agatacactt gaagctgtac tgacactgat
    33961 ccagaaccat tacggcattg atcctttttc tgctaaagaa aaattagcag agctagttca
    34021 aaggatcgaa gctgattatc cagatgcaat ccattacagc tattaccctt ctgaaatcgt
    34081 tcgagcgatt gaaaaaggat atttattaga gattcaagag ccgactgtca tccgagatgc
    34141 ctctgttctt gtggcactta actcagcgct cgaaccaaat gggatgttga atttaccgac
    34201 aggaatcatt cgtcgccatc cagattgcgt cattgtgatc actacaaatc gaaattacca
    34261 aggaaatcgc ccattaaatg aatctttaag agatcggatg caacatgcgg aaaaaatgga
    34321 tcttcctgcc ttagaagtca tggcagaacg tgcgatggcg aaaactatga tccaaaaaca
    34381 agaacttctc ttaaagatgg cagagattat ccgtttgttg gatgaaactg caaaagctaa
    34441 cgccatcaaa ggtgtagcag gaatgcgttc ttatttttat tgggcaaata cctataagca
    34501 aggacaagat ttactccagt cgatttatcc aaaagttttg tataaattat ccactgattc
    34561 agatgaattg gcaattcttg aacaagcttt gtctgacagt ggattgctac tggaacttca
    34621 ggacatattg agaaaagaaa aatggggtca tcctcaatct gagcatacaa aaggaagaac
    34681 cattagcgcc gaagaagcaa atgaacgaaa catcactgaa aatgctgaag aaattctgcg
    34741 cttagatgaa aagaaatcgt tgtctgctga agaaccaaat gagggagaag agaaacaaga
    34801 agaaacaata gaaaaaaacg agattcctcc tcaaatgcaa aaagaacgca gtagcgatat
    34861 cgaaggaaaa aatcaaaaag aacaacaaac aggagatgat tctcaagaaa tgtctgcttc
    34921 tgacatggaa gcacatgata aggaaatcaa gaagcaactg aataaagaag cgcgacaaat
    34981 catgaaggaa acgattcatg aaaaagaagg tctcatcgtt caccgaccag ctttccaagg
    35041 aaaaacgccg gaagcacgtg agctacacat gcaagtaatg ccaatagtag aatcacttag
    35101 ccggcaaatt ttagagttac tggataatga acaaacagag acgtaccaaa aaggaaaata
    35161 cgaaggtcag cggttcaatg caagcaaagt agcatatggt gatttgagaa attttgacaa
    35221 aaagaacccg ccacatgagc aaccttcttt agcggtagct gtacggatcg atgaatctgg
    35281 gtcaatgata cgggatgatc ggatcgaagc ggcaaaaaaa gcagcgattg ccattgccga
    35341 attttctaag aaagtcgata tcccattgtt gatttacgga gatacagcag accgttcgtc
    35401 aagagaaaag acttctttgt tttcttataa agaatttgaa gacggatttc aatggttaaa
    35461 tgaaaaatta gtcactatga aaccaaggca aaacaatcgt gatggtgctg cacttcgttt
    35521 agcagcagaa aaattaaaca gacaatccgc aacaaccaaa ctgttgctta atattagtga
    35581 cggtcagcca aaagcgttgc cggattatac aggcaaaaaa gcaaaagaag atatccagtc
    35641 tgttttgaaa gaatatgaac gtcaaggaat cctatttgtc tccgcagcta ttggccaaga
    35701 caaagaagaa atcaagtcga tctacggtga aagtcgtttt gtagatatta ctgacttgaa
    35761 tgaatttccc aagcaaatga ttcaactgat tgcccgctat ctatgataaa ataaagctca
    35821 acgaacgcac ttagcagatt cgttgagctt ttcgtttcta gttaccggta acaaggatac
    35881 ttctaaaata gaatcggtta taatcaagag ggaccgttaa aggagtgaaa cgatatgata
    35941 caagaacaaa aatggtggaa agaagcagta ggctatcaaa tctatcctgc tagtttcaaa
    36001 gatagcaatg gcgatggtca aggagacatc aatgggatcc gtgaaaaact taattatttg
    36061 aaagatcttg gtatcggctt tatctggatc aatccgatct atcaatctcc atttgtcgat
    36121 aatggttatg acgtaagtga ctatcaagat atagaagaga gattcggaac gatggaagat
    36181 tttgatttat tgctaaaaga agcacatgaa ttagggatca aagtgattat ggatcttgtc
    36241 atcaaccata gttcggatca gcatcaatgg tttgaagaat cgaaaaaatc taaagaaagc
    36301 ccgtatcgcg attattacat ttgggtcgac ggggtagatg gcaaagaacc gaataattgg
    36361 acatcgattt tcggcggttc tgcttgggag tattctcatg aaactggaca gtattatctg
    36421 catgtttttg caaaagaaca accagactta aattgggaaa gcgagaaatt gaaagaagaa
    36481 ctattcaata tgatccgatg gtggttggat aaagggatag atggatttcg tttagatgcg
    36541 atttctcatg taaaaaaaga tgaatattca gtaaaagcca cagagaaccc tttctcaccc
    36601 tttcaaaatg tctcaggaat cgaagaacat ttgaccgatt tgaaacatgt ttttgaagag
    36661 tacgatatca tgactgtcgg agaagcaagt ggtgtcactg cagaagaagg accacaatgg
    36721 gtagggaaag acggttattt cgatatgata ttcgaatttg atcatatcca tatctggcag
    36781 caagaaaaag aagggcaatt agatgtcctt aaattgaaac ttgcattaag cgcttggcaa
    36841 acttcattgg atggtatcgg atggaacgct ttatatatgg aaaaccatga tgttcctcgt
    36901 gccgtttcag tgtttggaga tactcgtccc gatttttggg ctatgtctgc aaaagcaatc
    36961 gctatgatgt atttcttttt gcaaggtacg ccatttattt atcaaggaca ggaaatcgga
    37021 atgacgaata tgccctttga atcgatcgat caagttgatg cagtagattc gaaaagactt
    37081 tataaaaggc tgcttgcgga aggtaagaca agagaagaag cattggatat cattcgtgaa
    37141 acaacgagag acaacagcag gacacccatg caatggactt cggaacaata tgctggtttt
    37201 tctacacatg aaccttggct gatcactaat ccaaatacca aaacgatcaa tgtagaacaa
    37261 caagaatatg aaccagagtc ggtcctgcaa ttctataaga acatgatccg cattagacaa
    37321 accaataaag gtctgatata cggttcttat aaagaatatc ttcatgaaca tccacaactt
    37381 tatgtctatg aaagatattt agaagatgaa caatatctta ttatggtaaa cttaacagaa
    37441 tcacttgctg actatgaact tccaaaagaa gcagatcagt cctggacatt actcttgtca
    37501 aattcctcta gcggagaatt tgaagctaaa ggcatactag caccttatga agctcggtta
    37561 tataaaacaa acaagtaaaa cttcctcgtt aaagttcatt agttggactt tgcgaggaag
    37621 tttttcagat tgctctgtca attactcatt tcaataattc gctctaggac caccgatata
    37681 tttaggacga gagcgaaggg ctgtcacgtg agcttgtcca agggttttca aacgaggtct
    37741 gacaggtact tggacatgtt tgatatgcat accgattgat gtgtcaccga tatcgatacc
    37801 ggcttgtcca actacgtgtt ccacttcgac aggatcattg aactggtcaa aagcagcaac
    37861 agaacaagca cctccagcat gtagtgcagg aacaacagag acaatttcga aattttttgc
    37921 ttctgccact gttcgttcta ctactaatgc tcggttcaaa tgttcacaac cttggacagc
    37981 tagcgcaatt tctttaggat ccaacaactc ttttaacgta cggatgatcc attgaccaac
    38041 ttccgcactg gaattttttc caatatgtcc tccaacgact tcgcttgtgc tgcatcctaa
    38101 aacaaaaaga tcaccttttt tcaagtgtgc ctcagccaat atgtcattaa ctatttcagt
    38161 caattggtct tttaattctt tttctgacaa cttcattttc taaaaccttc tttttcaatt
    38221 aatattgctt ccgtgcttga atacgttgaa gacttgcagt tataggaaag gcaagtccca
    38281 ttccaatcag attttggaag atgtttcctg gtatagaagc cactccagcc ggccaatcat
    38341 ataaaatcca accggcgata ccatagccga tgatcataac taagcttcct aataaaagcc
    38401 ctaatcccca catacgagta gaagatttgc tgaaagaacc agcaacatat ccttgtaacc
    38461 cgtggatcac taatgaaaaa actgcccact gcggatatcc tgaaatcaaa tcgatcaata
    38521 gaccactgga agcaccaaca actaatccac ccgttggacc ataaagaaaa gcagcggtat
    38581 agatcccggc ctcgcataat gtcacaaatc cattcgtagc cggtacaggg atcaaaattg
    38641 ttaatgaaag ggcaactgtc aacgcaatca gcatagcata gatcgttaat tgcttagttt
    38701 tcatttctca ttcctccttt ttgaataccg ccagaccaga cacttccggt atgatcgttc
    38761 atcagcacgc cattagaaat acaatgggca acaaattttt tactttctgc tactgctttg
    38821 gtaaaatctg cccctaacac aagattcgct gttattgcag aagaaaaggc acaaccggcc
    38881 ccgtttactg ttgtagtgtc gattttttta tgagaaaata cctcataatg atcaattgaa
    38941 tagagaagat ccacagcttc atctccagcc atccttgtgc cgcctttgat cacaatgttt
    39001 tttgttcctg aagcagtgag ttctttagcg agatggatca tttctgttaa cgaagaggga
    39061 agagcttgac cagaaagtaa agcagcttct gccaaattag gcgtaatcat cgtaacaagt
    39121 ggaaaaagtt cttcgatgag ctgcgaaata tacaattgat tgatcacagc ttcagtttct
    39181 ttgaatgcca aaacagggtc taaaatgatt gggatatttt gttttgtttt tatgaagtct
    39241 cgaacaacca tgattgcttc acttgaattc aataacccaa ttttgatacc atctaatgaa
    39301 gtcattttat caatcgaaac taactgtcgt ttaagcaatt ccaaagatat agattccagc
    39361 ataaatcgct cattaatatc tagtgcccct atagaagtca aggcgcttag tccgaataga
    39421 ccaaaattct caaaagtttt taaatctgtt tggattcctc caccagcaaa tggatcagaa
    39481 ccgccaattg tcaagattct tttcataaca ttttcctcca tgaataagag tataacgaaa
    39541 ttttcaaaaa agaaaaacag ccaattggca tatttttatc tcatccaatt taaaaaaaca
    39601 aaaagaaaag aaagaccaaa atggatcttt ctttccttaa atagttagga cttttgtgat
    39661 tttaattctt tctcgatttg aagccgttgc actttcattg tagctgttcg cgggattgca
    39721 tcaaaatcac ggatgatagg gacgttcaag cgaggaagtt catggacttg ctcccaccat
    39781 cgattccaat ccatttcttt atcaggagct aaagcaataa tcggttgtgg agaatgattt
    39841 ttatccctta caatcactac ttctgccaaa aaatctaacg aatctaataa aaagtcttca
    39901 atagctaatg tactattgat cgtttcgatc aaatccactt gccgatcttt caaaaatagg
    39961 tggccatgct tatccataaa accgtaatct ccgctatccc accaatcgcc ataaacattt
    40021 tcctggaacc gtgcatcttc cttgtaataa gtcaaagccc gtcctttaga cagtaattgt
    40081 atatgaccat ctgtgttttc aggtaggaca ttcccaactg aatctgtaat ccgagcgctt
    40141 gtcatgtctt ccagtccaac gcccatatct cttgcatcac ttgttttcaa actctctagt
    40201 gtatgagcct taagaatcat tggtccgcat tcactttggc catatacttg aagaaatacc
    40261 gcatcctgtg ccatcgacgt tcgcaaaaat gtcttcattg tctggttgtt tatagcatca
    40321 aaagtggagt gatagtatcg gatacccgaa aaagcttctg gatgctcctt agcagtaaaa
    40381 cgccactgaa caaagttatt cggatgggtc tctagtgcaa ttggccggtg agcttccaac
    40441 atatgaacaa cttttgaact ttgtgcgttt gctaaaggca tcataggaaa tcccatggac
    40501 atcaaagaag agataccaat attgaatctg gaatgcactg gagagatgtg aaaagcgatt
    40561 aatttctttt ccgaaatttt tgtgaaaact gttttttgcc atttcgtccg ccatcccata
    40621 gaatgagcag aatgacaaat aagtttaggg ataccagttg taccagaagt atgggtcata
    40681 taagaaattt catttttgtc taaaaatgat tgagaaacag acaaagcagg ttgttccatt
    40741 aaataaagaa taggaa
//
LOCUS       DAZKLD010000009        36421 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.009, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000009 DAZKLD010000000
VERSION     DAZKLD010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 36421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36421
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.009"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            25..696
                     /locus_tag="ACG8KR_000399"
     CDS             25..696
                     /locus_tag="ACG8KR_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFQ0199801.1"
                     /translation="MNFLVVLKDTLIERSTFLYVQLQENKTLLHFSPEFHLEGLTLGE
                     IYQAEGLSAVLHFFKVELELPVKDYIELSLESWDRAVVELFPEGLMIDTNDGKIHLTA
                     AELQKQMRYQIDDENSFSIFRRQQKILKSFLKVISKKRNLIKTTQLLKKYPGLLHTSI
                     QFPQLITMIHRFIRMQQLPSKKLFLPLTDTFRVVNREDKKKVIVIDFTRNRNVLHQVA
                     MEKAN"
     gene            755..1195
                     /locus_tag="ACG8KR_000400"
     CDS             755..1195
                     /locus_tag="ACG8KR_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0199802.1"
                     /translation="MMQTWLERLIRFQQNYHKLMQGRYAKFDQLNRGLLIAALVFSLL
                     SRWLPYRIGQLLFIVFFGWMIFRFLSKKIYPRLNENQRYLKYLDRVKEKYHQIKNKKQ
                     VKMDRKSYTFFECPNCHQKQRAPKGKGRIRVTCKTCGIKFETNV"
     gene            1207..1875
                     /locus_tag="ACG8KR_000401"
     CDS             1207..1875
                     /locus_tag="ACG8KR_000401"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HFQ0199803.1"
                     /translation="MKLVLLRHGESEANFENYWTGWLDVALTEKGKEQAAVAGQKMLE
                     AGLQFDAVYTSVLKRAILTGQVALEEMGQLWLPIIKTWRLNERHYGDLVGKNKDKMKE
                     IYGKDQVKTWRRGYTEVPPLTAENRFDRRYDHLDPHLIPYGESLEMTVNRIIPLWQDH
                     LAPKLKDRQDLLVVGHGNSIRALTKYLEDVPEDQMDTIDIPNAQPIQYTLADDLTILD
                     KKIL"
     gene            1939..2562
                     /locus_tag="ACG8KR_000402"
     CDS             1939..2562
                     /locus_tag="ACG8KR_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HFQ0199804.1"
                     /translation="MKNYIWDFDGTLFDTYPAMVDGAWQALKDFGISMDKKEIYFKMK
                     KYSTSYLINESNLNAREFNELFHRYEKESTEVSRPFPETKQVLEMLKDNGGRHFILTH
                     RLTESTWGLLKEHRLAHLIEEVVGIDQDFPRKPDPASLNYLIDTFHLERTDTMMIGDR
                     RLDIEAGKNAGVATCLYDIDHFLGEIPADYVVGNLNEILTLQSIIKK"
     gene            2618..3004
                     /locus_tag="ACG8KR_000403"
     CDS             2618..3004
                     /locus_tag="ACG8KR_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HFQ0199805.1"
                     /translation="MEIRRQKEVVEAFHYDMRLPNQEVETDLKVGFSPLKETIENYPK
                     EHSIVGARLEFRLVFDTYVLSGSVSQINHILNRKIEGQNDVTQEEVDELVAPLFDIVR
                     RMSYEVTEIALDQPGVKLNFQSEKQE"
     gene            3217..4608
                     /locus_tag="ACG8KR_000404"
     CDS             3217..4608
                     /locus_tag="ACG8KR_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HFQ0199806.1"
                     /translation="MTTLITVLVVLVFAALLYVFYRMQKRHTKFSTRVFTALGVGIVF
                     GGLLQLIFGVDDKVVTQSMEWIGIVGQGYVSFLQMLVMPLVFISIIGAFTKMKVSEKL
                     GKISATVLTSLLGTTAIAALIGIGSTMLFGLQGAKFTQGAAETARISELATRQETVEN
                     LSIPQQILSFIPKNVFADLAGSRATSTIGVVIFAAFVGIAYLGVKRKAPKEAAFFSDL
                     VDSLYKIIMRIVTLVLRLTPYGVFALMTNVLATSDFAAIVNLGKFVVASYVALLAVLI
                     VHSLILIGAKVNPINYFKKAFPVLSFAFTSRSSAGALPLNIETQTKALGVDDATANFS
                     ASFGLSIGQNGCAGVYPAMLATIVAPAAGVNVFSLEFILMLVAIVTISSFGVAGVGGG
                     ATFASLIVLGAMNLPVAIVGLVISVEPLIDMARTAVNVSGSMVAGIVTSARIKDLDRD
                     VINDDSKVIDVNA"
     gene            5219..5587
                     /locus_tag="ACG8KR_000405"
     CDS             5219..5587
                     /locus_tag="ACG8KR_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HFQ0199807.1"
                     /translation="MFTNEIKIMLYVDDVEKNATFWRAVGFAEKDRQEMDGTLIIEVG
                     VEDSQTSFLLYDRAFVESHSPGTATSAPSLMFYSKDIFGLYKKMQTQSVTLGDMVQLG
                     EEYVFNFADPDGNYFAVTGK"
     gene            5767..6708
                     /locus_tag="ACG8KR_000406"
     CDS             5767..6708
                     /locus_tag="ACG8KR_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HFQ0199808.1"
                     /translation="MDYQVLLYYNYTKIEEPEEFAKNHLAFCKSLDLKGRILVAEEGI
                     NGTVSGTVENTTAYMKAMKADPRFADTMFKIDPSEGHAFKKMFVRYRPELVSLKLEED
                     IDPNQVTGAYLSPQEFKEAILDEQTVVIDARNDYEYDLGHFRGAVRPDIRSFRELPDW
                     IREHKEEFMEKRVVTYCTGGIRCEKFSGWLVREGFKDVGQLHGGIATYGKDPEVQGEL
                     WDGKMYVFDERIAVDINQVDKQIVGRDWFDGTPCERYINCSNPECNRQILASIENEEK
                     HLGACSIECAKHPRNRYVQDHGISPEEAELRFTALIK"
     gene            6777..7508
                     /locus_tag="ACG8KR_000407"
     CDS             6777..7508
                     /locus_tag="ACG8KR_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFQ0199809.1"
                     /translation="MKSLIYAIGDVHGKHELFEKLIHYYDPKIHQLVLLGDLNDRGPR
                     TKECFLLGKQLVEETGAIYLRGNHEEYFLQFLQSPEDWYAPYMRNGGKETFESLIHPG
                     ASEEYSPTEISMMIQSRYSELIKFLVQRPLYYEWENYLFVHAGVDLTKKDWRKTSLRD
                     FIWIREPFHEGKNNTGKTIVFGHTITPMLHGDMQTTDLWIQDHKIGMDGGAVFGGSLH
                     GVVFDKEGILQDIEYQNNEGPWHGI"
     gene            7652..9832
                     /locus_tag="ACG8KR_000408"
     CDS             7652..9832
                     /locus_tag="ACG8KR_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HFQ0199810.1"
                     /translation="MTENLNQTIIQLVQKELSDYRPKQLTTVLNLLNEGNTVPFIARY
                     RKEMTGSLDEVQIREIEERYAYLENLEKRKTEVIRLIDEQGKLTPELEAEITQAVKMQ
                     QVEDLYRPYKQKRRTKATIAKEKGLEPLAKWLMQLTDGEVQSEAEKYIDKEKEVSSAE
                     EALHGAHEIIAEQVSDNAKFRTWIRSYTYNKGMYVSNVKDEQADEKGVYEMYYDFAEP
                     VHKMVSHRILATNRGEKEDILKVFFQVDEAAILAYLDRQIVKNPASPSSSFVREAYQD
                     SYKRFIQPAIERELRNELTEKADEQAIAIFGENLRNLLLQPPLKGKVVLGFDPAYRTG
                     CKLAVVDATGKVLAIEVIYPHKPAAQAKREAAGPAFIQLINKYQVDMVAIGNGTASRE
                     SELFVAEQLKSADHKTYYAIVNEAGASVYSASEIARKEFPHLQVEERSAVSIARRLQD
                     PLAELVKIDPKAVGVGQYQHDVSQKRLAEQLDFVVETAVNQVGVDVNTASPQLLQHIS
                     GLNKTTAQNIVIYREENGEFTARTQLKKVPRLGPKAYEQAIGFLRVPGGKNILDNTGI
                     HPESYSIAKEILTSVQLSEKELGTEEAVEKLTRLSVEKLAESLSVGEETLADILAGLT
                     QPGRDMRDEMPAPLLRTDVLSMEDLKPGMELTGTVRNVIDFGAFVDIGVKQDGLVHIS
                     KLSKKFVKHPTDVVSVGDIVTVWIEQVDTKKGRISLTMLSPYEE"
     gene            9852..10334
                     /locus_tag="ACG8KR_000409"
     CDS             9852..10334
                     /locus_tag="ACG8KR_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HFQ0199811.1"
                     /translation="MTQEELQQLVEEISLKSFQLPFKHQAMFNPRLRTTGGRYHLNDH
                     HLDFNAKLFEESDEETIAGIIKHELCHYHLHLAGRGYQHKDMDFKYLLKKTGGLRYAP
                     ALTKKQIKMEYYRCQDCSATICRKRRIDVRKYRCGRCHGEIKWIETKREDFNEAQKIR
                     "
     gene            10350..11111
                     /locus_tag="ACG8KR_000410"
     CDS             10350..11111
                     /locus_tag="ACG8KR_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HFQ0199812.1"
                     /translation="MEKSEQRNLKDWNQLLNFLAAPEEGNFEELTAPTLILAGNCLPI
                     LADKAAQMYLNGQVDQLFLVGGVGHATRILYENFEKQGFHFEDGMSESEICRQYLKEV
                     YDLPDKAFLIESKSTNSGENATFSLEILHSLGAVPEKILLMNDPTLQRRTRATFEKVW
                     QNEQTIFANAVPFVPEILHFSEEIIFTAKELNHQWPKEYFHALVLGEMERLHDDENGY
                     GPKGKDFIPHIDISEEVWSSYTRIKESIKTDFSRT"
     gene            11242..12576
                     /gene="murC"
                     /locus_tag="ACG8KR_000411"
     CDS             11242..12576
                     /gene="murC"
                     /locus_tag="ACG8KR_000411"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HFQ0199813.1"
                     /translation="MENQNKLYHFVGIKGSGMSSLALVLHEQGLNVQGSDIEKYFFTQ
                     RDLEKANITILPFNADNVKPGMTIIAGNAFPDSHEEIQRAKELGLEVIRYHDFIGHFI
                     QNYTSIAVTGSHGKTSTTGLLSHVLSGVRPTSYLIGDGTGHGDPQAEFFSFEACEYRR
                     HFLAYSPDYAIMTNIDFDHPDYYTSIDDVFTAFQTMAGQVKKAIFAYGDDAYLRKLKA
                     NVPIYYYGVTENDDIQARNIERTTSGSAFDVYHGDEFVGHFTVPAFGKHNILNALGVI
                     AVAYFEKLDLKEVAEEMLTFPGVKRRFSEKIVADMTVVDDYAHHPAEIKATIDGARQK
                     YPDKEIIAVFQPHTFTRTIALMDEFAEALDLADKVYLCDIFGSAREEQGNVKIEDLGA
                     KIKKGGEVIKENNVSPLLDYHDAVVIFMGAGDVQKFEQAYEKLLSSTTKNVL"
     gene            12883..13368
                     /locus_tag="ACG8KR_000412"
     CDS             12883..13368
                     /locus_tag="ACG8KR_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HFQ0199814.1"
                     /translation="MNIGKPRKLGKTINEIEEGDSLSLTESIEDNQLLLYLGLTNDAN
                     PLYIQHDFAQKTEYQQPIVPSVMLMGIITSAISKHLPGPGSHVVNFSVNFIEPVFHYE
                     TLTFQFEVIKVDKMKDVVTISVEAENTNEDRVLDAVVMVQPPQVPELEIAIESEGELN
                     E"
     gene            13361..14044
                     /locus_tag="ACG8KR_000413"
     CDS             13361..14044
                     /locus_tag="ACG8KR_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HFQ0199815.1"
                     /translation="MNNPSTSMSGINRFYAKVYGFLGMGLALSAVTSYLALWVFPLQL
                     ATFINNFPLGFVGLWIVELILVMVLGVKAQKNPSLAIGGFIAYSLLNGIILAVTLAYY
                     DIGTVTQAFLTATLMFMGMSVVGVTTKRDLSGVGRAGQAALIGVIIALVLNIFILRSS
                     VVELFISLLLVVIFAGITAYDNQSIRNIYVQTNGTAHSGIAVFMALKLYLDFINLFLA
                     FLRIFGRNN"
     gene            14170..16815
                     /gene="polA"
                     /locus_tag="ACG8KR_000414"
     CDS             14170..16815
                     /gene="polA"
                     /locus_tag="ACG8KR_000414"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HFQ0199816.1"
                     /translation="MTKNKLLLVDGNSVAFRAFFALHNSLERFKNRNGLHTNAIYAFN
                     TMFENVMQKEQPTHVLVAFDAGKTTFRTEMYAEYKGGRSKTPGEFKEQMPYIRDLLTG
                     LGVQYYELPNYEADDIIGTLAEKVDKDQFDVVVLSGDRDLTQLASKEVKVDITVKGVS
                     DIESYTPEHVAEKYDGLTPKQIIDMKGLAGDASDNIPGVTKIGEKTAIKLLKQYGSVE
                     GIYEHIDEMKQSKMKENLINDKEQAFLSKKLATINTSAPVDVSIDSLKYEGKNLDKLV
                     PFYKEMDFNQFLSKLNIVEEEVKMDDILFEVVHEFKEEMFTTDMALYVEMMGDNYHTE
                     EIVGVAWGTEKKIYVTNDLSVFENRAFHSWITDPTRLKKVYDAKRTYVALNRYTGKTK
                     GIDFDVLLGAYLLDTNEKSTDIEGIAAHYGYNDIQSDEAVYGKGAKKGLPEEEELFFA
                     HLARKVAAINALTPKLSQELAEKNQEDLFRKMELPLSIILAEMEISGIKVDATRLQEM
                     KGEFSARLREIEQKIYEEAGEEFNLNSPKQLGVILFEKMGLPVIKKTKTGYSTAVDVL
                     EQLREQAPIVEDILTYRQIAKIQSTYVEGLLKVIQSDGKVHTRYVQTLTQTGRLSSVD
                     PNLQNIPIRLDEGRKIRQAFVPREKDWLIYSSDYSQIELRVLAHISNDEHLKAAFLEG
                     QDIHASTAMRVFGIEKAEDVTPNMRRQAKAVNFGIVYGISDYGLSQNLGISRKAAQQY
                     IDTYFEKYPGVKEYMERIVREAKDQGYVETLYHRRRYLPDINSRNYNIRSFAERTAIN
                     TPIQGSAADILKIAMIELDKRLKETGLQATMLLQVHDELVFEVPEKELESLDKLVKEV
                     MEQAVSLHVPLITDSSWGKTWYEAK"
     gene            16860..17696
                     /gene="mutM"
                     /locus_tag="ACG8KR_000415"
     CDS             16860..17696
                     /gene="mutM"
                     /locus_tag="ACG8KR_000415"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HFQ0199817.1"
                     /translation="MPELPEVETVRKGLERLVVGKTIQKVQVLWPKIIEQPETPIFEA
                     SLVGETIQSIGRRGKFLIFHLDHCELISHLRMEGKYQFTKENTPIDKHTHVLFFFEDG
                     SQLRYNDVRKFGRMTIVEKGASATYRGIMKLGPEPLPDSFLLADFANGLKKSHKAIKP
                     LLLDQRLVTGLGNIYVDEALWEAKIHPEQPANTLRPKEVEQLRLSIIDVLDRAIEAGG
                     TTIRSYLNALGESGGFQVALHVYQQTGKPCIRCGTPIVKTKVAQRGTHYCPNCQRLKI
                     AK"
     gene            17660..18289
                     /gene="coaE"
                     /locus_tag="ACG8KR_000416"
     CDS             17660..18289
                     /gene="coaE"
                     /locus_tag="ACG8KR_000416"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340744.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HFQ0199818.1"
                     /translation="MSELSKAKNRKVGFVLGLTGGIATGKSTAAKVFQSHGFPLIDGD
                     VIARETVEPGTLALRKIVSVFGQEVLQPDGQLDRGKLGMIVFPSKEKRQKLDQLLDPF
                     IRKAIKDQIASLSSNHPLVIVDIPLLYEGHYDHYMDAVAVVYTTPETQLQRLMKRNQL
                     TLEQAQQRISSQLPIEEKKQRADILFDNNGTKEILVGQIENWLFANRFL"
     gene            18341..18673
                     /locus_tag="ACG8KR_000417"
     CDS             18341..18673
                     /locus_tag="ACG8KR_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HFQ0199819.1"
                     /translation="MGSLKNQKDKVVGNAKEKIGEMLNKENLIDEGRTQSETAEEKEK
                     QYQARIDEIEDQQAEEEAKRQDLTQERKNPTPDRSKTPLPESDHIPDEQKIEEQRMKH
                     YTGIEEKL"
     gene            18985..19476
                     /gene="nrdR"
                     /locus_tag="ACG8KR_000418"
     CDS             18985..19476
                     /gene="nrdR"
                     /locus_tag="ACG8KR_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HFQ0199820.1"
                     /translation="MRCPRCQHNNSRVIDSRQADDGRAIRRRRECENCSFRFTTFERI
                     EAAPLLVIKKNGEREEFNRDKILRGLIRSAEKRPVAMEQMEQIVNHVENRVRSLGENE
                     VSTNLIGEYVMEDLVDLDEIAYIRFASVYRQFKDMSVFLKELQDIVDKAKENNKENNK
                     ENN"
     gene            19499..20872
                     /locus_tag="ACG8KR_000419"
     CDS             19499..20872
                     /locus_tag="ACG8KR_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HFQ0199821.1"
                     /translation="MENAWKELQPKNIYQGRKAAPLREDSKNALLYLYQPLIGGEALS
                     IYFTLLSEISQESGKGPEGMHADLLSSLGCGIPQFYDARKKLEGIGLLDVYFKEDSDL
                     GPRFIYELLEPLIPKAFFKDSVLAFLLLEKVGERRFQKLTQFFEPKRHTAEGYQKITK
                     KFLDVYRFNEAVYAANQENIESLNKSFEESESVALLEADSTFDWQFLNDWLAKKHIAS
                     LDDPIIKQVDMYHQLYGFDELTLGELISQSYDFSEQKVSEKELQKAILAMQRRPVKQT
                     KETVAVSENSNEPSSNEHLTNAALQLIKEAQSIPPMKYLEAIKSEKGGYVSKQENWLL
                     QDLVSQSGLSSSVINVLLNYVLVIKNNASLNASYVNTVANEWAQKKIATAEEAIQHIR
                     QISNQAKQSKQKKQTNYQTGKRNVRREKLPDWVNQPKDEKQISSEKKAEIDRRFKEYL
                     AKKEGDI"
     gene            20872..21798
                     /gene="dnaI"
                     /locus_tag="ACG8KR_000420"
     CDS             20872..21798
                     /gene="dnaI"
                     /locus_tag="ACG8KR_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HFQ0199822.1"
                     /translation="MEDVGKELKRIISKRNYQEKYAEMIAEVLNDADVQAFLSAHQEE
                     LTQADIEKSYAKLYEFVQEKRKYQKNDPTMIAPGYEPKLTLNFHYVDVTYVPTEALLA
                     RQRQEEIRSRVKAMDMPKDIQSASFENFERTDGRGYASLEALDFVEKYTADPKGYHKG
                     LYLVGSFGIGKTYLLGATARELAIAGFTTTLVHFPTFAVEMKQAIGKDQVAEKLDAVK
                     RSPILMIDDIGADAMSSWIRDEVFGVILQYRMQEQLPTFFSSNFTMDELEQHLSVTQR
                     GDEEPLKAKRIMERIRYLTKEIEMTGRNRRNG"
     gene            21927..22367
                     /locus_tag="ACG8KR_000421"
     CDS             21927..22367
                     /locus_tag="ACG8KR_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFQ0199823.1"
                     /translation="MRAIFSSISVVGLFFLLAGCGSADSNTGSVDSIDAGQTVKTSST
                     KTERQSIFVGTIESITQVEAEEETLQLKLVDVMAKEDPANMGTSFSNDGVILNATAEK
                     ISVDINELKIGDKVQFTLVEMPIMTMSIPPQVPGDSIIDVSLNK"
     gene            22511..23212
                     /locus_tag="ACG8KR_000422"
     CDS             22511..23212
                     /locus_tag="ACG8KR_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine(22)-N(1))-methyltransferase TrmK"
                     /protein_id="HFQ0199824.1"
                     /translation="MNHLDLSQRLAAVGDFVPESARLADIGSDHAYLPVALILRGKIE
                     YAVAGEVVQGPYESARKQVKKDGLEDRITVRLANGLDAIQPQDGITAVTIAGMGGSLI
                     RDILEAGKLNQRLSGQERLILQPNIGERTLRSWLQENQYKIQSEKILEENKKIYEIIV
                     AEKEQKPVTYNEMELMFGPFLLEEKSEIFKSKWTRELQQRKAIREQLKQAANQEARQK
                     QIEQEIQMIEEVLKP"
     gene            23209..24330
                     /locus_tag="ACG8KR_000423"
     CDS             23209..24330
                     /locus_tag="ACG8KR_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HFQ0199825.1"
                     /translation="MTISGRTFIQKFESYCPQWLAEEGDPVGLHIGTLDKPIQRVMMT
                     LDVRPEVVEEAIKKKIDLLIAKHPPIFRPVKRLVTDQPQEKMYADLLKHDIAVYAAHT
                     NMDIIWDGLNDWFCELLGIEVESYLVKTHEIHYKKLAVYVPVDHAQKMREVLAAAGAG
                     TQGDYTGTSFTSIGHGRFTPEAGAQPAIGKVGKTEQVQEAKVEVILPETIEKQVIQAM
                     RSAHPYEEPAYDLFAIDEPVEMFGLGRVGELPQEISIEAFVEQVKEAFQLDGLRIVQP
                     KNAKSSVKRIAICGGSGEKFYPQAIAQRADVYITGDIYYHTAHDMQSAGLIAIDPGHY
                     IESLCKQRFIEKFESWKQEENWDINFFVSETNTNPFQFN"
     gene            24345..25577
                     /gene="pepT"
                     /locus_tag="ACG8KR_000424"
     CDS             24345..25577
                     /gene="pepT"
                     /locus_tag="ACG8KR_000424"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HFQ0199826.1"
                     /translation="MYENLLPRFLRYVKTETRSDATSKTTPSTQTQVAFAQELKKELE
                     ELGLADVFYNEDNGFVMGTLPSNVDHEVRSIGFISHMDTADFNAVGVNPQIIENYDGE
                     STIVLDAEERYTLNTKDFPNLKNYEGHTLITTDGSTLLGADDKSGIAEIMTAMEILIN
                     NPSIPHGTIKVAFGPDEEIGVGADKFDVEQFDVDFAYTVDGGPLGELQYETFSAAQAN
                     ITIQGKNVHPGTAKDTMINALQLAIDFHNQLPADEVPEKTEGYEGFFHLMALDGNPEE
                     ASMSYIIRDHDREKFEARKAQITHIQEELNQRFDQERIKVDMYDQYYNMKEIIEKDMS
                     IVELAKQAMIELGISPVIEPVRGGTDGSKISYMGIPTPNLFAGGENMHGRFEFVSLQV
                     MEKATDVIVKIAELNTKS"
     gene            25709..26617
                     /locus_tag="ACG8KR_000425"
     CDS             25709..26617
                     /locus_tag="ACG8KR_000425"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFQ0199827.1"
                     /translation="MEKLLVFYNKSSGKDEGEKLARWFQDYVKEKQPELYVELAETGP
                     SIDDAELVKKAEAIEADTLVIIGGDGTIHHIVQAFQKTLSQYHVGLLPGGTVNNLARV
                     LEIPLKKEEAADVILEGNVRKIDYGQVNDEVIISTLTIGILADTAVWISQEEKQRYGS
                     WIFIKKFFKLLLKKKKYHLDLETDEEHWKGKSQLLTVTMSNSVGGFTNFDDSATLDDG
                     KFHVTIVPSLDIFRFIFYLPRIMRGKFYTIPGITYFTAKRMKIQAQEKSVGTRTDGDT
                     TDDLPVELSVVSKGLTVYSPKKEEKA"
     gene            26652..26924
                     /locus_tag="ACG8KR_000426"
     CDS             26652..26924
                     /locus_tag="ACG8KR_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HFQ0199828.1"
                     /translation="MDVLLHNPLWFYLFIVNFYLFCLMGYDKHQARTSGWRVPENNLI
                     FVSLLGGGLGGLISRKVFHHKTRKKKFTIGFAAGVLVDLILIYFFH"
     gene            26935..27981
                     /locus_tag="ACG8KR_000427"
     CDS             26935..27981
                     /locus_tag="ACG8KR_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhN family protein"
                     /protein_id="HFQ0199829.1"
                     /translation="MSSKGTKTKIILNLLLLIVIFAIIYYLINQSFADIFAELLSTSV
                     VVLAAAVILGTVYQIAEGRSIKEIAEPFAPNFTTKDGFLTSSYIAFYRIVSFGTGTLI
                     SEIYFYNKKGMKLSQGAGVTALHMIMYKLAVITYALAGLVIQFSLFYSNGPSMIWFII
                     AGILLTIVIVAFLLAMSVSLNVQIFFVSLSNKLFKSERLRKWVDTCNQQIYSLRETVQ
                     TIIQDRTALLRIYLWNMLKLVFWYVLPYLILIENHPKIDFLLTISFISFAVVLSGVIP
                     TPAGIGSFEFVYLLLFRPVVGTVDAVSSLLLYRFSSFILPFLYGFVYVIAERRRVIKE
                     EIIEVKKENKENQG"
     gene            28232..30841
                     /gene="clpB"
                     /locus_tag="ACG8KR_000428"
     CDS             28232..30841
                     /gene="clpB"
                     /locus_tag="ACG8KR_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HFQ0199830.1"
                     /translation="MNIEKMTTTLQEAIAEAQQIAVTRKHQDIDIAHVWKIFLQSNHF
                     GRNFYTDAGLDVESFEHEIDRLLDEYPVVSGGNVQYGQNLSQNFFRLLNEADQIRESF
                     GDEFLSTEVVILALMKLKNYPLTVYLNKNGLSEKELRKNIEEMRGGERVTSQNQEEQY
                     KALEKYGVDLVQQVKSGKMDPIIGRDEEIRDVIRILSRKTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVRKDVPENLKDKTIFSLDMGALIAGAKFRGEFEERLKAVLKEVKKSDGRIL
                     LFIDEIHNIVGAGKTEGSMDAGNLLKPMLARGELHLIGATTLDEYRQYMEKDKALERR
                     FQKVLVKEPTVEDTISILRGLKERFEIHHGVNIHDNALVAAATLSDRYITDRFLPDKA
                     IDLIDEASATIRVEMNSMPTELDQVTRRLMQLEIEEAALKKESDDASKKRLKNLQEEL
                     AELREEANAMKMQWETEKEEVNSVSAKRAEIDKAKHELEDAENNYDLERAAVLRHGTI
                     PQLEKELKELEAKAKDSEIKMVQESVTENEIAQVVGRLTGIPVTKLVEGEREKLIKLN
                     ETLHKRVIGQDEAVDAVSDAVIRSRAGLQDPNRPLGSFLFLGPTGVGKTELAKALAEN
                     LFDSEDHMVRIDMSEYMEKHAVSRLVGAPPGYVGYEEGGQLTEAVRRNPYTIVLLDEI
                     EKAHPDVFNILLQVLDDGRLTDSKGRVVDFKNTVLIMTSNIGSQLLLEGVTADGTIPE
                     AVAEQVNTLLRGNFKPEFLNRIDDTILFTPLSLDNVKGIVDKMVAQLAQRLEHQEILL
                     TISDEAKTWIAENAYEPAYGARPLKRFITKEVETPLAKEIVAGHVMPKSKVTITLLDG
                     QLHFKTEELEEIV"
     gene            complement(30992..31678)
                     /locus_tag="ACG8KR_000429"
     CDS             complement(30992..31678)
                     /locus_tag="ACG8KR_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0199831.1"
                     /translation="MEGLSVIKEIKKVQIALLAFGVFVVCYNFYEFITQKYSTSQGIT
                     FIVESLLGIALIFMPQVILKVFKLKIPAAIVLFYWFFLFISVFLGTGMHLISIISFWD
                     KILHAVSPMVLTALGYGLIGYLMKDAEISKTSPWLFLLFGFAFAGLCGVFWEFWEFLC
                     DQFLGMNLQRFAASDGTLFVGRAALMDTMGDLLTNTIGAALMGLFAWSQSKKDERYFE
                     SYKLEKVKNT"
     gene            complement(31656..32150)
                     /locus_tag="ACG8KR_000430"
     CDS             complement(31656..32150)
                     /locus_tag="ACG8KR_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside 2-deoxyribosyltransferase"
                     /protein_id="HFQ0199832.1"
                     /translation="MKIYFAAPMFAKSDLLYNQQLVKEIRELSSELSIYLPQENEAIN
                     DKTAYADSRMIALADTEKVLASDLMIALLDGLTIDAGVASEIGVAYAKGIPVIGLYTD
                     TRQQGGTHPKKIAALQETAENQFHYLNLYTIGLIKLNGKVVSSEIELLSEVKRFLDGG
                     TFSD"
     gene            complement(32170..33390)
                     /locus_tag="ACG8KR_000431"
     CDS             complement(32170..33390)
                     /locus_tag="ACG8KR_000431"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0199833.1"
                     /translation="MDQKDFRENLELKPVTQEHIDQFNELLSYVFQVTEADIEESGFE
                     NKRAFIRSKKPILEVSKVFGWFHENKLISQIAIYPCEVNIHGARYKMGGVTGVGTYPE
                     YANHGLMQDLIIVALDNMRKNKQWISYLYPYSIPYYRRKGWEIMSDKLSFKIRDTQLP
                     KQIPVSGMVERLPVDHPDVFSVYDKFAQQNHGALFRSDFHWEEYWRFENEDERTAAVY
                     YDSNHEPRGVLFYWVAEEIFHVKEMFYLDQEARNGLWNFISAHFSMIYWVHGDIYKNE
                     PLAFLIEDSQIKEQIEPYFMARIVDVKEFLQRFPFVGTADAFHFIIEDPVAPWNNGVF
                     ALTWDEQGQVRVLNEPIGKSVRLDIQTLTCLMMNYRRASYLARIERLETDEETLKSLE
                     RIIPNMEAYFSDYF"
     gene            33537..35372
                     /gene="lepA"
                     /locus_tag="ACG8KR_000432"
     CDS             33537..35372
                     /gene="lepA"
                     /locus_tag="ACG8KR_000432"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HFQ0199834.1"
                     /translation="MDIKKMKERQEQIRNFSIIAHIDHGKSTLADRILEMTHTVTSRE
                     MQDQLLDSMDLERERGITIKLNAVELNYTAKNGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAVLVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRQEIEDVI
                     GIDASEAVLASAKSGIGIEDILEQIVEYVPAPSGDLDAPLKALIFDSIYDSYRGVVLN
                     VRITDGMVKPGDKIKLMSNGKTFDVTEVGVFSPKAVARDFLMVGDVGYITASIKTVQD
                     TRVGDTVTLADNPAAEALPGYRKMNPMVYCGLYPIDTSRYNDLREALEKLQLNDAALQ
                     FEPETSQALGFGFRCGFLGLLHMDVVQERLEREFNLELITTAPSVIYHVNKTDGTTAT
                     VDNPADFPEPVTIQDVEEPFVKAQIMVPNDFVGAVMELSQRKRGEFITMDYLDDYRVN
                     VVYNIPLSEIVFDFFDKLKSSTKGYASLDYEMSGYQKSKLVKMDILLNGEKVDALSFI
                     VHRDFAYERGKAIVEKLKKLIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKEGKKRMKQIGSVEVPQEAFMAVLKMDEQDSVK"
     gene            35568..>36421
                     /locus_tag="ACG8KR_000433"
     CDS             35568..>36421
                     /locus_tag="ACG8KR_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0199835.1"
                     /translation="MVDNSSGRVLKSLRKEKKLSQKKLADLAGISQSTLVKYEKGSRK
                     IPKDVDNTLSKILNIETLIKDEEDKIEILIGKLIAYRDMNKLLNKELADNIGISEVLL
                     SYVLNRKRNPSKEMQKKIDIFLLSNEKEILKEINRDSEIFSLSKDDKIVMGKRIREVR
                     KNREETLEKFGKNFTIYTGKNVISRWEKGINIPDIEKLMNIAYLGKVTVPYLMYGEDY
                     KNILPKDERVSDFKKINSFSMGLRMRKIRKDYYLEREEFGKLFSPSISKWSIDRYENG
                     RDIPNTNR"
BASE COUNT        12272 a         6349 c         7802 g         9998 t
ORIGIN      
        1 aatgatgaaa aaagggggag aaaaatgaat tttttagttg ttttaaaaga tactctgatt
       61 gagcgatcta catttctata tgttcaattg caggaaaata aaacattgct gcatttttca
      121 ccagagttcc atctagaggg gctaacatta ggagaaatct atcaagcaga agggctgtcg
      181 gctgttttgc acttttttaa ggtagaattg gaattaccag taaaagatta tatcgagctt
      241 tctcttgaat cttgggatcg agcagtggtt gaactgtttc cagaaggttt aatgatcgat
      301 acaaacgatg ggaagatcca tctgactgca gcagaactgc aaaaacaaat gcgttatcaa
      361 atagatgacg aaaattcttt ttctattttt agacgtcagc aaaagattct aaaaagcttt
      421 ttaaaagtga tcagtaaaaa acggaatctg atcaaaacga cacagctgtt gaaaaaatat
      481 ccaggtctgc ttcacacgtc tattcaattt cctcaattga ttacgatgat ccatcgtttt
      541 atacgtatgc aacagctacc atctaagaag ctgtttcttc cattgacgga cacatttcga
      601 gtagtcaaca gagaagataa aaagaaagtg attgtgattg actttaccag aaatcggaat
      661 gtccttcacc aagttgcaat ggaaaaggcc aattaaggct ttttcttttt ggattttttt
      721 gtcataataa atattagatg atggaggaga taatatgatg caaacatggt tagaaaggct
      781 aatacgattt cagcagaact atcacaaact tatgcaggga agatatgcga aatttgatca
      841 gttgaatcgc ggtctattga tcgctgcgct tgtctttagt ttgctaagtc gttggttgcc
      901 ctatagaatc ggacaattgt tgttcatcgt ctttttcgga tggatgatat ttcgtttcct
      961 atcaaaaaaa atctacccca gactaaatga gaatcaacgt tatttgaaat atcttgatag
     1021 agtcaaagaa aaatatcatc agataaaaaa caaaaagcaa gtcaaaatgg atcgaaaaag
     1081 ttatactttt tttgagtgcc cgaattgtca tcaaaagcag cgtgcaccta aaggaaaagg
     1141 acgaatacgt gtgacatgca aaacatgtgg tataaaattc gaaacgaatg tttaagggag
     1201 aaaagaatga aattagtatt attacgtcat ggcgaaagcg aagcaaattt tgaaaattac
     1261 tggacaggat ggctagatgt cgccttaaca gaaaaaggaa aagaacaagc agcagttgcc
     1321 ggtcaaaaaa tgcttgaagc cggacttcaa ttcgatgccg tttatacctc tgttttgaaa
     1381 cgtgcgattc ttaccggaca agtcgcacta gaagaaatgg gtcagttatg gcttcctata
     1441 ataaaaacat ggcgtctaaa tgaacggcat tatggggatc ttgtgggtaa aaacaaagat
     1501 aagatgaaag aaatatacgg aaaagatcaa gtaaaaacat ggcgcagagg atatacggaa
     1561 gttccgccgt taaccgcaga aaatagattt gaccgcagat atgatcactt ggatccgcat
     1621 ttgatcccat acggagaaag tctggaaatg acagtgaatc ggatcattcc cttatggcaa
     1681 gatcatctag caccaaaact aaaagatcgg caagatttac tagttgtggg tcatggtaat
     1741 agcatccgag cactgacaaa atatttagaa gatgttcctg aagatcaaat ggatacgatc
     1801 gatatcccaa atgcacaacc aatccagtat acattagcag atgatttgac aattctggac
     1861 aaaaaaatac tttaaagaac acctttatct aaaagtgaga gatagaaaaa agaagcaaag
     1921 gaatggaaca aaaggaacat gaaaaattat atatgggact ttgatggtac attgtttgat
     1981 acgtatcccg caatggtaga tggtgcatgg caagcactaa aagattttgg tatcagtatg
     2041 gacaaaaaag aaatctactt caaaatgaaa aagtattcta ctagttattt gatcaatgaa
     2101 tcaaacttga atgccagaga gtttaatgaa ttatttcacc gatatgaaaa agaaagtacg
     2161 gaagtttccc gtccatttcc agaaacgaaa caagtactgg aaatgctaaa agacaacggc
     2221 ggtcgtcatt tcatcctgac ccaccgtttg acagaatcaa catggggcct gttaaaagaa
     2281 catcgattgg cacatctgat tgaggaagta gtagggatcg atcaggactt tcctcgtaaa
     2341 cctgatccag catctttaaa ttatttgatc gatacttttc atttagaaag aacagacacg
     2401 atgatgatcg gcgatcgtcg tttagatatc gaagcaggga aaaatgcagg agtcgctact
     2461 tgtctttacg acatcgatca ttttctgggt gagatcccgg cagattatgt ggtaggaaat
     2521 ctgaatgaaa tccttacctt gcagagcatc attaaaaagt agtatcccct ttcaaaggag
     2581 atatgttata attttcataa aggagtgata aaattatatg gaaatcagaa gacaaaaaga
     2641 agttgtagaa gcatttcatt atgatatgag attgccgaac caagaagtag aaacagattt
     2701 gaaagtaggc ttctcgcctt taaaagaaac gatcgaaaac tatccgaaag aacatagcat
     2761 cgtaggagca cgcttagagt tccgtttagt gtttgataca tatgttttat ctggaagtgt
     2821 gagtcagatc aatcatatcc tcaatcggaa aattgaaggt caaaatgatg tcacacaaga
     2881 agaagtggac gagctagtag caccgttatt cgatatcgta cgccgaatgt cttatgaggt
     2941 gacagagatc gccttagatc agcctggggt aaaattgaat tttcaatcag aaaaacaaga
     3001 atagacagta aaaacatacc tgcgatagat taaagctatc acaggtatgt ttttttatta
     3061 ctaattgtaa gcaaaaagaa tgtcatttcg ataaaaatcg tctaaaatat aaaagtcact
     3121 gaaaaaaatt ttttcaggag gatggttgaa gaatcattct cgggaacctt tacttccttc
     3181 gatggacttg tcattaataa tatgaggagg agaaaaatga caacactcat cacagtttta
     3241 gtggttctag tttttgcggc attattatat gtgttctatc gtatgcaaaa gcgccataca
     3301 aaattttcaa cacgcgtctt tacagcatta ggagttggta tcgtctttgg cggtctgctt
     3361 caattgatct ttggtgtaga cgacaaagtc gttactcaat caatggaatg gatcgggatt
     3421 gtaggtcaag gatatgtatc attcttgcaa atgctagtta tgcctttagt gtttatttcg
     3481 atcattggtg cattcacaaa aatgaaagtt tctgaaaagc tagggaaaat tagtgcgact
     3541 gtattgactt ctttacttgg aacaacagct attgcagcat tgatcggaat tggttcaaca
     3601 atgctgttcg gactccaagg agctaaattc acacaaggag ctgcagaaac agcacgaatc
     3661 tctgaactgg caacacgtca ggaaacagtt gaaaacttat cgattccaca gcagatttta
     3721 tcctttatcc ctaaaaacgt ttttgctgat ttagcaggta gtcgtgcaac aagtacgatt
     3781 ggagtcgtta ttttcgcggc atttgtcgga atcgcttatc taggcgtgaa aagaaaagcg
     3841 ccaaaagaag cagcattctt ttcagattta gtcgacagtc tgtataaaat cattatgcgg
     3901 atcgtgacgc ttgttttgcg tttgacacct tatggagtat ttgctttgat gacaaacgtt
     3961 ttggcaacaa gtgattttgc agctatcgtc aatttaggta aatttgtcgt tgcttcttac
     4021 gtagcattgc ttgctgtttt gattgtccac tcactgattt tgattggtgc aaaagtcaat
     4081 ccaatcaact atttcaaaaa agcctttccg gtattgagct ttgcgtttac ttcacgttca
     4141 agtgctggag ctttgccatt gaatatcgag actcagacaa aagctttagg agtagatgat
     4201 gcaacagcta atttctcagc aagttttggt ttgtctattg gacaaaacgg ctgtgctggc
     4261 gtttatccag ctatgttggc aacgatcgtc gcaccagcag ctggagtgaa tgtctttagt
     4321 ttggaattta ttttgatgct tgtggcaatt gtaacaatca gttcttttgg tgtagctgga
     4381 gtcggtgggg gcgcaacatt tgcttcactg atcgttttag gggcaatgaa cttgccagta
     4441 gcaatcgttg ggttagttat ctctgttgaa ccattaatcg atatggcacg tacagcagta
     4501 aacgtcagcg gtagcatggt agctggtatc gtgacaagtg cacgcatcaa agatttagat
     4561 cgcgatgtca tcaatgatga ttctaaagtg atcgatgtga atgcatagtc aaaaagaggt
     4621 tgtgatagaa gtggtcagct ttaagaaata agaagaagtt tcctaaaaat agtttctcaa
     4681 atttttgtga aatttcggct tatttccgag aagctacttc tgaaacaccg tgaataagag
     4741 gttgtgaaaa agctgccctg catcaagtaa taagaacagc caaacaaaaa tagttcctca
     4801 tattttacgt ttggttgagc ttattaccgc agatgcaagc ttttgaacac cgtttaagaa
     4861 agaggtcgtg agcctgccgt ttcactctga gtcacaaaga gcaatttcaa aaaatagctt
     4921 ctcatgtttg ttgaatattg tgtcttgtga ccgaggagtt gtcaggagaa caccgtttac
     4981 cagaggttgt gacttgtttt tagtcacgac ctcttttttt atttattcca tataaataca
     5041 cattatttat tccgaaaagt gaaagcgcaa ataacaaaaa caaccaactt atttattatt
     5101 aaagaaaagc gtatttaagt aggacgattc tgttgagagc tttcgtagat tttcccattt
     5161 tcctatggta aactgacaaa gttgagaatg ttagaacaga tcgtttagga ggaaacagat
     5221 gtttacgaat gaaataaaaa tcatgcttta tgtggacgat gtagaaaaaa atgcaacgtt
     5281 ttggagagca gttggctttg cggaaaaaga tcgtcaagaa atggatggga cgcttataat
     5341 tgaagtagga gttgaggaca gtcaaacctc atttctgtta tatgatcgag catttgttga
     5401 gtcccattca cctggcacag ctactagtgc cccatctttg atgttttaca gtaaagatat
     5461 ctttggacta tataaaaaaa tgcagacgca atcagtcaca ttaggtgata tggtacagct
     5521 tggagaagaa tatgtcttta actttgctga tcccgatggc aactactttg cggttacagg
     5581 taaataatag aaataaagac tttgtttttc tgtgcgaaac aggaaagcga agtcttttta
     5641 attcaaataa taatattatg taaacaaaaa actttccatg cgtaattata cacaagcaaa
     5701 taaatcgtca aatgcgaacg ccttctttat actgtttagg aacgaaaaga aggaaggaag
     5761 aaatacatgg attatcaagt actgctttat tacaattata caaaaatcga agaaccagag
     5821 gaatttgcca aaaatcattt agccttttgc aaatctttgg atcttaaagg tcgtattcta
     5881 gtagctgaag aaggaataaa tggtactgtg tctggaaccg ttgaaaacac gactgcctat
     5941 atgaaagcaa tgaaagctga tcctcgattt gctgatacga tgttcaagat cgatccaagc
     6001 gaaggacatg cctttaagaa gatgtttgta agataccgtc cagagcttgt aagtttaaag
     6061 ctagaagagg acattgatcc aaaccaagtg acaggtgcct atctttcacc gcaagaattt
     6121 aaagaagcta ttttagatga acagacagtg gtgattgatg cacggaatga ttatgaatat
     6181 gacttgggac attttcgagg ggctgtacgc ccagatatac gcagttttcg agagcttcca
     6241 gactggatcc gcgagcacaa agaagaattt atggaaaaac gggtagtgac ctactgtact
     6301 ggtggcattc gttgcgagaa gttttctggt tggcttgtcc gtgaaggatt caaagatgtc
     6361 ggacagctgc atggcggtat tgctacttat ggtaaggatc ctgaagtcca aggagaatta
     6421 tgggacggca agatgtatgt gtttgatgaa cggatcgctg tcgatatcaa ccaagtagat
     6481 aaacaaatcg tcggtcgcga ctggtttgac ggaacaccat gtgaacgata tatcaattgt
     6541 agcaatcctg agtgcaatcg tcagattctt gcttccattg aaaatgaaga aaaacattta
     6601 ggagcatgta gcatagagtg tgcgaaacat ccgcgcaatc gctatgttca agaccatgga
     6661 atctctccag aagaagcaga actgcggttt acagctttaa ttaaatagaa aacaatagtt
     6721 ttgattgtcg accatttcca atatgcgcta tactagcgtt gaggatggtg atccacatga
     6781 aatcattgat ttatgctatt ggggatgtcc atggcaaaca tgagcttttt gaaaaactta
     6841 ttcactatta tgatccaaag atccatcagc ttgttttatt aggggatctc aatgatcgag
     6901 gtcctcgtac aaaagaatgt tttttattag gaaagcaatt agtcgaagaa acaggagcca
     6961 tttacctccg gggaaaccat gaagaatatt ttttgcaatt tctgcaatca ccggaggact
     7021 ggtatgcgcc ttatatgaga aatggtggaa aagaaacgtt tgaaagcctg attcacccag
     7081 gggcatcgga ggaatactcc ccaactgaaa tctcgatgat gatccaatcg agatattcag
     7141 aactgattaa atttttagtc cagcgcccct tgtattatga atgggaaaat tatctttttg
     7201 tccatgccgg tgtggatttg acaaagaaag actggcgtaa gacaagtcta agagacttta
     7261 tttggataag ggaaccattt catgaaggga aaaacaatac aggtaagaca attgtatttg
     7321 gacatacgat tacaccaatg ctccatggag atatgcagac aacagacctc tggattcagg
     7381 atcataaaat cggaatggac ggtggggctg tattcggagg gtcattgcat ggggttgttt
     7441 ttgacaagga aggtattctt caagatattg aatatcagaa caacgaagga ccttggcatg
     7501 gaatctgact aatggaaaga atgaaggagc tgtgactttg ttgcggctct ttttattttt
     7561 atagagaata ttgtttcttt cagtagttta acccaggata aaaagtatga tagaataaag
     7621 aagcaatttt tagcagaaaa gaggcatttt aatgacggaa aatttgaacc agacgatcat
     7681 tcagcttgta caaaaagaac tttcagacta tcgtcctaag cagttgacta ctgtacttaa
     7741 tttgttaaat gaaggaaaca ctgttccttt tatcgcgcgt taccgaaaag aaatgactgg
     7801 aagtcttgat gaagtccaga ttcgtgaaat cgaagagcgg tatgcctatc ttgaaaactt
     7861 agaaaaacgt aaaactgaag tgattcggct aatcgatgaa caagggaaac tgactcccga
     7921 gttagaagca gaaatcaccc aagccgtgaa aatgcaacaa gtggaagatt tgtatcgtcc
     7981 atacaaacaa aaacgacgga caaaagcaac gattgctaaa gaaaaaggac ttgaaccact
     8041 cgcgaagtgg ctgatgcagc ttacagatgg agaggtgcaa tctgaagcag aaaaatatat
     8101 tgacaaagaa aaagaagtct cttctgccga agaagcgttg cacggtgctc atgagattat
     8161 tgctgaacaa gtcagcgata atgcaaaatt tcggacttgg attcggtctt atacgtataa
     8221 caaaggtatg tatgtcagca atgtcaagga tgagcaagca gatgaaaaag gcgtatatga
     8281 aatgtattat gactttgcag aacccgtcca taaaatggtt tctcatcgaa tattagctac
     8341 taatcgtgga gaaaaagaag atattctgaa agtattcttt caagtagatg aagcagcaat
     8401 tttagcttac ttggatcgcc aaatagtcaa aaatcctgcc tctccttctt cttcattcgt
     8461 cagagaagcc tatcaggata gttataaacg ctttattcag ccagcaatcg agcgagaact
     8521 gcgcaatgaa ttgactgaaa aggcagatga gcaagcaatc gctattttcg gcgaaaacct
     8581 ccgtaatctt ttgttgcaac cgccactaaa aggtaaagtt gtcctgggat ttgatccggc
     8641 ttatcgtact ggatgtaaac tagcagtcgt agatgcgaca gggaaagttt tagcaatcga
     8701 agttatttat cctcataaac ctgcagcgca agcaaaaaga gaagctgctg gaccagcttt
     8761 tatccaatta attaataaat atcaagtaga tatggtagct atcggaaatg gtacagccag
     8821 tcgggaatct gaactttttg tggctgagca gttgaaatcc gccgaccata aaacctatta
     8881 cgctatcgtc aacgaagcag gcgcttctgt ttactccgct agcgaaattg cacgaaaaga
     8941 atttccgcat ctacaagttg aagaaagaag tgctgtttct attgcccgtc gcctgcaaga
     9001 tcctttagct gaattagtca aaatcgatcc taaagctgtc ggagtaggcc agtaccaaca
     9061 tgacgtttct caaaaacgtt tggctgaaca attggatttt gttgtggaaa cagcggttaa
     9121 ccaagtaggt gtggatgtga atacagcaag cccgcagctc ttgcagcata tatcaggatt
     9181 gaataaaaca actgcccaaa acattgtcat ttatcgtgaa gaaaacggag aattcaccgc
     9241 acgaactcaa ttgaaaaaag tgccacgctt aggaccaaaa gcatatgaac aagcaatcgg
     9301 attcttgcga gtccctggtg ggaaaaacat tttggacaac acaggtatcc atcccgaaag
     9361 ttattctatt gcaaaagaaa tattaacttc agtccaactt tcagaaaaag agcttggtac
     9421 agaagaagca gtagaaaaat tgacacgatt atccgtagag aaactggctg aaagcctatc
     9481 agtcggagaa gaaacgttag ccgatatttt agctgggctg acacaacctg gacgagatat
     9541 gcgtgatgag atgccagcgc ccttattacg tacagatgta ttgagtatgg aagacttgaa
     9601 accagggatg gaattgactg gaacggtccg taatgtcatt gacttcggcg catttgttga
     9661 tatcggtgtg aagcaagacg gacttgtcca tatatcgaaa ctcagcaaaa aattcgtcaa
     9721 gcatcctaca gatgtcgtgt cagtaggaga tatcgtaaca gtttggatag aacaagtcga
     9781 tacgaaaaaa ggcagaatca gtttgacgat gctttcccct tatgaagagt aacaatgagg
     9841 agtaacaaaa attgacacaa gaagaactgc agcagttagt agaagagata tcattaaaaa
     9901 gcttccagct tcctttcaag caccaagcaa tgttcaatcc acgcttacgt acaactggcg
     9961 ggcgctatca tttaaacgat catcatttag attttaatgc gaagctattt gaagagtcag
    10021 acgaagagac gattgcagga atcatcaaac atgaactttg ccattatcat cttcatctag
    10081 ctggaagagg gtatcagcat aaagatatgg atttcaagta tcttttgaaa aaaacaggag
    10141 ggttgcgcta tgcaccagcc ttgacgaaga aacagataaa gatggagtat tatcgatgcc
    10201 aagattgttc tgccacgatc tgccgcaaga gaagaatcga tgtacgcaaa tatcgttgtg
    10261 gaagatgtca tggagaaatc aaatggattg agacaaaaag agaagacttt aatgaagcac
    10321 aaaaaatcag atgaaaggat agaaggcctt tggaaaaaag tgagcagagg aatctaaaag
    10381 actggaatca gctattaaat tttttagccg cacctgaaga gggaaatttt gaggaactga
    10441 cggcacctac acttatttta gctggaaact gtcttcctat cttagctgat aaagcagcac
    10501 aaatgtattt aaatggccaa gtagatcaat tgtttttggt cggaggggtg gggcatgcga
    10561 ctagaatcct ttatgagaat tttgaaaagc aaggatttca ttttgaagat ggcatgagtg
    10621 aaagtgaaat ctgtcgtcag tatctaaaag aagtctatga tttgcccgat aaggcttttc
    10681 tgatagaatc gaagtctaca aattctggag agaacgccac tttttcactg gagattcttc
    10741 attccttagg agctgtacca gaaaaaatct tattgatgaa tgatccgaca ctgcaacgca
    10801 ggactcgagc aacttttgag aaagtatggc aaaatgaaca aactattttc gcgaatgctg
    10861 tgccatttgt gccggaaatc cttcactttt ctgaagagat catctttaca gcaaaagaac
    10921 tgaatcatca atggcctaag gaatatttcc atgcgctagt attaggagaa atggaacgtc
    10981 tccatgacga tgagaacggc tatggtccga aaggaaaaga ttttattcct cacattgata
    11041 tatcagagga agtttggtct tcttacacca gaattaaaga gtcaataaaa acggattttt
    11101 ctcgtacttg accattttag caagcgaatc ctcctttcca aataaatttg gttgaaattt
    11161 tcaatcgttt ataagataat gaggagcgtg ttgaatgtaa tagctaaaag aatggacgct
    11221 agataagaga agaggattca aatggaaaat caaaataaat tgtatcattt tgttggtatc
    11281 aaaggttcag gaatgagctc cttggctctt gttttacatg aacaaggtct aaatgtccaa
    11341 ggatcagaca ttgaaaaata tttctttaca caaagagatt tagaaaaagc aaatatcacg
    11401 attctgccat ttaacgcaga taatgtaaaa ccagggatga cgatcattgc aggaaatgca
    11461 tttcctgatt cacatgaaga aatccagcga gcaaaagaat taggtttgga agtgattcgc
    11521 tatcacgact ttattggtca ttttatccaa aattatacga gtatcgctgt aacaggctct
    11581 cacggaaaaa caagtacgac tggactgctc tctcatgtat tgagtggtgt ccgtccaaca
    11641 agttatctaa ttggagatgg aacaggacat ggtgatccgc aagcggaatt cttttcattt
    11701 gaggcctgtg aatatcgccg tcatttcctt gcctattcac cagactatgc aatcatgacc
    11761 aatatcgatt ttgatcatcc ggattattat acaagtatcg acgatgtatt tacagctttc
    11821 caaacaatgg ctggacaagt gaaaaaagct atttttgcgt atggtgacga tgcttattta
    11881 cgcaaattaa aagcaaatgt gccgatttat tattatggtg tcacagaaaa cgatgatatc
    11941 caagcacgaa acatcgaacg cacaacttct ggttctgctt ttgatgtcta tcatggggat
    12001 gaatttgttg ggcacttcac tgtaccagca ttcggtaaac acaatatctt aaatgcttta
    12061 ggtgttattg cagtagctta tttcgaaaaa ctggatctga aagaagttgc agaagaaatg
    12121 ctgactttcc caggagtaaa acgtcgattc agtgaaaaga tcgtagcaga catgaccgta
    12181 gtcgatgatt atgcgcatca ccctgcagag atcaaagcaa cgattgatgg cgcacgccaa
    12241 aaatatccag ataaagaaat aattgcagtt ttccaacccc atacgtttac gcgtacgatt
    12301 gcattgatgg atgaatttgc agaagcactt gatttagctg ataaagttta cttatgcgat
    12361 atttttggtt cggctcggga agaacaagga aacgttaaga ttgaagacct tggagcgaaa
    12421 atcaaaaaag gtggagaagt catcaaagaa aataatgtat ctccattgtt agattatcat
    12481 gatgcagtag taatcttcat gggtgctgga gatgttcaaa aatttgaaca agcgtatgaa
    12541 aaacttttaa gcagcacgac gaaaaatgta ctgtaataca attactgcag tgtccttata
    12601 aaaatagaaa gagggggagt ggcaaaaatc ttgccaagcc cccttttttg caacttatga
    12661 taaacggtgt tcaaaagctt gcatctgcgg taataagccc aatcaaatca aaaacatgag
    12721 aagctgtttt ttcttggctg ttcttattat ttgatgcagg acagcttttt cacgacctct
    12781 gtaaactata ggaatctgtt aaatcaaggg agcagaactt gccttttacg ctacttttgg
    12841 tacaataatg tatgattaaa aataaactgg agggtttcta tcttgaacat aggtaagccg
    12901 agaaaactag gaaaaacaat aaatgaaatc gaggaaggcg actcgttatc cttaacagaa
    12961 tctattgaag ataaccagct tttgctgtat ttgggattga cgaatgatgc aaatcctttg
    13021 tatatccaac atgactttgc acaaaaaaca gaatatcaac agccaatcgt tccatcagtg
    13081 atgctgatgg ggatcatcac aagtgcaatt tcaaagcatc ttcctggacc aggatcacat
    13141 gttgtcaatt tttctgtcaa ttttattgaa ccggttttcc attatgaaac actgactttt
    13201 cagtttgagg tcataaaagt agataagatg aaggatgtgg tgacgatttc tgtagaagcg
    13261 gagaatacga acgaagatcg tgtgttggat gctgtcgtta tggtccaacc gccacaagtt
    13321 cctgaattgg aaatagcaat cgaaagtgaa ggtgaattga atgaataatc caagtacatc
    13381 aatgtcaggt atcaatcgtt tttatgcaaa agtttatgga tttttaggta tgggacttgc
    13441 ccttagtgca gtcacttctt acttggctct ttgggtattt cctttgcaat tagcaacttt
    13501 tatcaataat tttccactag gctttgtggg tctgtggatc gttgaactga ttttagtaat
    13561 ggttctaggc gtaaaagcac aaaagaatcc gagcttagca atcggtggat ttattgccta
    13621 ctctcttttg aatggcataa tattagcggt tactcttgca tactacgata ttggaacagt
    13681 aacgcaagct ttcttgacag ctactttgat gttcatgggg atgtctgttg tcggagtaac
    13741 aacaaaaaga gatcttagcg gggttggaag agcaggacag gcagctttaa tcggagtgat
    13801 cattgcacta gtcttgaaca tcttcatttt gagaagtagc gtagttgagc tgttcatctc
    13861 cctactgtta gtcgtcatct ttgcagggat tacagcttat gataaccaaa gcatccgtaa
    13921 tatctatgtc cagacaaacg gaacagcaca tagtggaatt gctgtattca tggcattgaa
    13981 gctgtatttg gactttatca acttgttctt ggcgtttctg agaatctttg gacggaacaa
    14041 ctaaaaaaga aaaaaattca tcagttgggg ctataccgaa ttatttggta tggtcccgct
    14101 ttttcttttc gcattccgca ggattctttg ttagaatgaa taagaacttt acagtaagga
    14161 gttaagaaga tgacaaaaaa taaattatta ctcgttgatg ggaacagtgt ggcttttcgc
    14221 gcattctttg cgcttcacaa ttctcttgaa cgtttcaaaa atagaaacgg tcttcataca
    14281 aatgcaatct atgcattcaa tacgatgttt gaaaatgtta tgcaaaaaga gcaaccgact
    14341 catgttctgg ttgcgttcga tgcaggaaaa acgacttttc ggacagaaat gtatgcggag
    14401 tacaaaggtg gaagatccaa aacacctggc gaattcaaag aacaaatgcc atatatacgt
    14461 gatttgttga caggactagg ggtacagtat tacgaattgc ccaattatga agcagatgat
    14521 attatcggta ctttggcaga aaaagtagac aaagatcaat ttgatgtcgt cgtgctttca
    14581 ggtgatcggg atttgactca attagctagt aaagaagtaa aagtggatat cactgtaaaa
    14641 ggtgtcagcg atatcgaaag ctatacacca gaacatgtag cggaaaaata tgatgggttg
    14701 acgccaaaac agattattga catgaaggga ctagccggag atgcctctga caatatcccc
    14761 ggtgtgacta aaatcggtga aaaaacagct atcaagctat tgaaacaata tggctcagtc
    14821 gaaggaattt atgaacatat cgatgaaatg aagcaaagca aaatgaaaga aaatctgata
    14881 aatgacaaag agcaggcttt cttatcgaaa aaattggcga ccatcaatac ttcagcgcct
    14941 gtggatgtct ctatcgactc attgaagtat gaagggaaaa atctcgataa actcgttccg
    15001 ttttataaag aaatggattt caatcagttc ctttctaaat tgaatatcgt agaagaagaa
    15061 gtaaaaatgg atgatatttt gtttgaagtc gttcatgaat tcaaagagga aatgtttact
    15121 acggatatgg cgttgtacgt ggaaatgatg ggcgacaatt atcacacaga ggaaatcgtc
    15181 ggagtagcgt gggggacaga aaagaaaatt tatgtcacga acgatctatc tgtattcgaa
    15241 aaccgagcgt tccattcttg gatcactgat ccgactcggc tgaaaaaagt atatgatgcg
    15301 aaacgaactt atgttgcttt aaaccgctat actggaaaaa cgaaaggaat cgattttgat
    15361 gtcttgctag gtgcatacct tctcgatacg aatgaaaaaa gcacagatat cgaaggaata
    15421 gctgctcatt acggatacaa cgatatccaa tcagatgaag ctgtatacgg caaaggggcg
    15481 aaaaaaggtt taccagaaga agaggaactc ttttttgctc atttagcgcg aaaagtagct
    15541 gcaatcaatg cgttgacacc aaaattgagc caagaactgg cagaaaaaaa ccaagaagat
    15601 cttttccgga aaatggaact gccattgtct atcattttgg cagagatgga aataagcggg
    15661 atcaaagtag atgcgactcg cttacaagaa atgaagggcg agttctctgc tcgcttaaga
    15721 gagatcgaac aaaaaatcta cgaagaagcc ggcgaagaat tcaatttgaa ttcaccaaag
    15781 caattaggag tgatcttatt tgaaaaaatg ggattacccg ttatcaaaaa aacaaaaact
    15841 ggatattcca cagccgtaga tgtactggaa cagcttcgtg aacaagcacc aatcgtagag
    15901 gatatcctca cctatcgtca aattgcaaaa attcaatcta cctatgtcga gggactgctg
    15961 aaagtgatcc aatcagatgg aaaagtgcat acccgttatg tccagacatt gacccaaaca
    16021 ggacgattaa gctctgttga tcctaatttg cagaacatcc cgattcgatt agatgaaggg
    16081 agaaagatac gtcaggcatt tgttcctaga gaaaaagact ggctgattta ttcttctgac
    16141 tattcacaaa tcgagttacg cgtacttgcg catatctcaa atgatgaaca tttgaaagct
    16201 gccttcctag aaggtcaaga tatccatgcc agcacagcga tgcgtgtatt tggaatcgaa
    16261 aaggcagagg atgtcacacc aaatatgcgt aggcaggcaa aagcagtgaa ttttggtatc
    16321 gtgtatggca tctctgatta cggactttca caaaatctag gaatcagccg aaaagcagca
    16381 cagcaataca tcgatacgta ctttgaaaaa tacccaggtg tcaaagaata tatggaaaga
    16441 atcgtacgcg aagcaaaaga ccaaggctac gtcgaaacgc tgtatcaccg ccgtcgatat
    16501 ctgccagaca tcaattcaag aaattacaat atccgttctt ttgcggaacg aacagctatc
    16561 aatacaccaa tccaaggaag tgctgctgat attttgaaaa tcgcgatgat cgaattggat
    16621 aaacgattaa aagaaacagg tcttcaagct acgatgctgt tacaagttca tgatgaactt
    16681 gtcttcgagg taccggaaaa agaacttgaa tcattagata aattggtgaa ggaagttatg
    16741 gaacaagctg tttctcttca tgtaccactg atcactgaca gcagttgggg aaaaacgtgg
    16801 tacgaagcaa aataaacatt gtttaagaca gagcaatata atgaaggtga aaagaaaaaa
    16861 tgccagaatt accagaagta gaaaccgttc gtaaaggatt ggaacggtta gtagtaggaa
    16921 aaacgattca aaaagttcag gtcttgtggc ccaagatcat cgagcaacca gagacaccca
    16981 tctttgaggc ttctcttgtt ggcgaaacca tccaatccat tggacgtaga gggaagtttt
    17041 taatttttca tctcgatcat tgtgaactga tctcacactt gcgaatggaa ggaaaatatc
    17101 aatttacgaa agaaaataca ccaatcgata aacatacgca tgtacttttc ttttttgagg
    17161 atggaagcca acttcgctac aacgatgtac gaaaatttgg tcgtatgaca atcgtcgaaa
    17221 aaggagcaag tgcaacttac cgaggaatca tgaaattggg acctgagcct ttgccagata
    17281 gcttcttatt agcagatttc gcaaatggac tgaaaaagtc ccacaaagca atcaagccat
    17341 tgctgctaga ccaacgtcta gtgactggtc taggtaatat ttatgtagat gaagcattat
    17401 gggaagcgaa gatccatcca gaacaaccgg cgaacacact gcgccccaaa gaggtagaac
    17461 aattacgtct atcgatcatt gatgttttgg atcgtgcaat agaagccgga ggtaccacga
    17521 ttcgcagtta tttgaatgca ttaggagaat caggcggatt tcaagtagca ctgcatgtct
    17581 atcagcaaac tggcaaacca tgtatacgtt gtggaacgcc gatcgtgaaa acaaaagtgg
    17641 cacaaagggg gacgcactat tgtccgaatt gtcaaaggct aaaaatcgca aagtaggatt
    17701 tgttttaggt ttgacgggtg gaatagctac cggtaaaagt acggcagcca aagtctttca
    17761 gtctcatgga tttcctttga tcgatggcga tgtgattgcc agagaaactg ttgagcctgg
    17821 aacgcttgct ctgaggaaaa ttgtatcagt gtttggccaa gaggtattgc agcctgatgg
    17881 gcaactagat cgagggaaac ttgggatgat tgtttttcca tcaaaggaaa aacggcaaaa
    17941 acttgatcag ctattagatc cgtttatccg aaaagcgata aaagatcaaa tcgcctctct
    18001 aagttccaat catcctttag taattgtaga tatcccattg ctctacgaag gtcattatga
    18061 ccactatatg gatgcagtag ctgtcgttta tactacgcca gaaactcagc ttcagcgttt
    18121 gatgaaacgt aatcaactga cgctagaaca agcgcagcag agaattagta gtcaacttcc
    18181 aatcgaagaa aaaaagcagc gtgcggatat tttgttcgac aacaatggaa caaaagaaat
    18241 acttgtggga caaatcgaaa attggctttt tgctaatcgc tttctttgag tatctgtgtc
    18301 aattttgcta tcctgagtat atagaaagtg agggatagcg atgggatcat taaaaaatca
    18361 aaaggataaa gtcgtaggta acgcaaaaga aaaaatcggc gagatgctca ataaagaaaa
    18421 tctaatcgat gaaggtcgta cgcaatcaga aacagcagaa gaaaaagaaa agcagtacca
    18481 agcaagaatc gatgaaatag aagaccagca ggcagaagaa gaagcgaagc ggcaagattt
    18541 gactcaagaa cgaaaaaatc cgacacctga tcgtagtaaa acaccgctcc cagaaagtga
    18601 ccacattccc gatgagcaaa aaatcgagga acagcgaatg aaacactata caggaatcga
    18661 agagaagtta taaaatagta aaaaatcgag atgtgacttg aagcagatga gaaggtgttc
    18721 atctgcttca agttttttgt gctagatgac taaatcagct tttgtgtaag ttatttctca
    18781 gtaatcaagg tgatcagtat tggaaaattg aaatttagta aggacagggg acacaattac
    18841 gcatgaaagt agaaaaaatt gttcttatat ccgtgttttt tgggtaggac agtcgcgttt
    18901 gtgatttgtt tcgtgttata atacaactag ttgtaaagaa agacacatcc tttatataaa
    18961 atgcgatgca agaggtgaga caaaatgaga tgtcccagat gtcaacataa taattcacga
    19021 gtaatcgaca gccgtcaagc agatgatgga cgtgcgatcc gtcgccgtcg cgaatgtgag
    19081 aattgttcgt tccgattcac tacatttgaa cgaattgaag ctgctccgtt attagtaatc
    19141 aagaaaaacg gggaacgcga agagttcaat cgcgataaaa ttttaagagg actcatccgt
    19201 tcagcagaaa aaagacctgt agctatggaa caaatggaac aaatcgtcaa ccatgtcgaa
    19261 aaccgcgtac gcagtctcgg agaaaacgaa gtatcgacga atctaattgg tgaatatgtg
    19321 atggaagatt tagttgattt agacgaaatc gcttatattc gttttgcaag tgtttatcgc
    19381 cagttcaaag atatgtctgt tttcttaaaa gagttgcaag atatcgtgga taaagcaaaa
    19441 gaaaataata aagagaataa taaagaaaac aattaagaaa caaaaaggag gcatttcctt
    19501 ggaaaatgct tggaaagagc ttcaaccaaa aaatatttac caaggaagaa aagcggctcc
    19561 gctgagagaa gacagcaaga acgccttgct gtatctttat cagccactaa tcggcggaga
    19621 agccttgagt atctatttca ccctgttatc agaaatctct caagaatctg gcaaaggtcc
    19681 tgaagggatg catgcagatc tgcttagtag tctaggctgc ggtatcccgc aattttatga
    19741 tgcaagaaaa aagctagaag gaatcggctt actggatgtt tattttaaag aagatagtga
    19801 tctgggacct cgttttattt atgagctgtt agaaccactc attcctaaag catttttcaa
    19861 agactctgtt ctggcttttc ttcttctgga aaaagttggt gaacgacgat tccagaagct
    19921 tacccaattc tttgaaccta aaaggcatac agcagaggga tatcaaaaaa tcacaaaaaa
    19981 atttttagat gtttatcgct tcaatgaagc agtgtacgct gctaatcaag aaaacatcga
    20041 atcgctgaat aaatcttttg aagaatctga atcagtagca ttacttgaag cagattcgac
    20101 attcgattgg cagtttttga atgattggct ggcaaaaaaa catattgcaa gtcttgatga
    20161 tccaatcatc aaacaagtcg acatgtatca tcagctttac ggtttcgatg aattgacact
    20221 tggcgaactg atcagtcaat cttatgactt tagtgaacaa aaagtatctg aaaaagaact
    20281 gcaaaaagcc attttggcaa tgcagagacg tccggtgaag caaacaaaag aaacagttgc
    20341 ggtttctgaa aacagtaatg aaccctcgtc aaacgaacat ttgacaaacg cagcgcttca
    20401 gctgataaaa gaagcacaga gcattccacc gatgaaatat ctggaagcaa tcaagtcaga
    20461 aaaaggcgga tatgtttcta aacaagaaaa ctggttactg caagatcttg tttctcaatc
    20521 aggtctttcc agtagcgtga tcaatgtttt gctgaattat gtactagtga tcaagaataa
    20581 tgctagctta aatgccagtt atgtaaatac agtggcaaat gaatgggcac aaaagaaaat
    20641 cgccacagca gaagaagcga tccagcacat tcggcaaata agtaatcaag caaaacaatc
    20701 aaaacaaaag aagcaaacaa attatcaaac tggcaagcga aatgttcgac gggaaaagct
    20761 tcctgattgg gtcaaccagc caaaagatga aaaacagatc agcagcgaga aaaaagcaga
    20821 gattgaccgc cgttttaaag aatacctggc aaaaaaggag ggtgatatct agtggaagac
    20881 gtaggcaaag aattgaagag gatcatcagc aaacgcaatt atcaagaaaa gtatgctgaa
    20941 atgatcgctg aagtcttaaa tgatgcggat gttcaagctt ttctaagcgc acaccaagaa
    21001 gaattgacac aagcggatat tgaaaaaagt tacgctaaat tgtatgaatt cgtgcaagaa
    21061 aagcgtaaat atcaaaaaaa cgatccaacg atgatcgctc ccggctatga gccgaagctg
    21121 acgttgaatt ttcattatgt ggatgtcact tatgtaccaa cggaagcatt gttagcacgg
    21181 caaagacaag aagaaatcag aagccgagta aaagcaatgg atatgccaaa agatattcaa
    21241 tcggcttctt ttgaaaactt tgaacgtaca gatggacgag gatatgcgag cttagaagcc
    21301 ctagattttg tagagaaata tactgctgat ccaaaagggt atcataaagg actgtatcta
    21361 gtaggaagtt ttggtattgg aaaaacatat ttattaggag caacagcgag agaattagcg
    21421 attgctggat tcaccacgac gctagtccat tttccgacat ttgccgttga gatgaaacag
    21481 gcaatcggaa aagatcaagt ggcagaaaag ttagatgcag tgaaacgttc acctattttg
    21541 atgatcgatg acattggtgc agatgcgatg agtagttgga tacgagacga agtattcggt
    21601 gttatccttc aatatcggat gcaggaacag ttaccaacct tcttctcatc gaattttaca
    21661 atggatgaat tggaacagca tttgtccgtc actcaacgtg gagatgaaga gccgctaaaa
    21721 gcgaaacgga taatggaacg catacgttat ttgacaaaag aaatcgaaat gactggtcgc
    21781 aaccgacgca atggttgaac agttgacaag tccaactgac atttgaccta gtagtaaaag
    21841 caggtcagcg aatcattata tggacagaaa tgaccggaga caatcaccgc aacaagtaat
    21901 gcagaaactt aaacggagga ttttttatga gggctatttt ttctagtata tctgtagttg
    21961 gtctattttt tcttttagca ggctgcggta gcgcagatag caacacaggt tctgttgatt
    22021 cgattgatgc gggtcaaaca gtaaaaacaa gcagtacgaa aacggagaga caaagcatat
    22081 ttgtcggaac gattgaaagt atcactcaag tagaagcaga agaagaaacg cttcagttga
    22141 aactcgtcga tgtcatggcc aaagaagatc cagcgaatat gggaacgtca ttcagcaatg
    22201 acggagtgat cttaaatgct actgctgaaa aaataagcgt ggacataaat gaattgaaaa
    22261 tcggtgacaa agtccaattt acattagttg agatgccgat catgacgatg tctatcccac
    22321 cgcaagttcc tggagattcg attattgatg tttcattgaa caaataacca ataacatttt
    22381 ttcaaaaata agaaaatgtt gttttataga acgaaaaggt atatttttta aggagatatg
    22441 ggaatttttt tattcctagt ctccttattt tatttggaaa atgctataat tatctagaag
    22501 gagtgaagct atgaaccatt tagatttatc tcagcgctta gccgcagtgg gtgactttgt
    22561 ccctgaatcc gctcgtttag cagacattgg atcagatcac gcttacttac ccgttgcatt
    22621 gatattaaga ggaaaaatcg aatatgcagt tgctggtgaa gtcgttcaag gtccttatga
    22681 atcagcacgt aagcaagtca aaaaagatgg attagaagac cgtatcaccg tacgccttgc
    22741 aaatggtcta gatgctatcc agccacaaga tggtattaca gctgtcacga ttgcgggtat
    22801 gggaggttca ctgatccgag atatcctaga agctggcaag ttgaatcaac gcttaagcgg
    22861 acaagaacgt ttgatccttc agccaaacat cggagagcgt acattacgat cttggctgca
    22921 agaaaatcag tataaaatcc aatcagaaaa aatacttgaa gaaaataaaa aaatatatga
    22981 aatcattgta gccgaaaaag aacaaaaacc agttacttac aatgaaatgg agctgatgtt
    23041 cgggccattt ctgcttgaag agaaaagtga gatcttcaaa tcaaaatgga caagagagct
    23101 tcaacaaaga aaagctatcc gtgaacagct caaacaagca gctaatcaag aggcacgtca
    23161 aaaacaaatc gagcaggaaa tccaaatgat tgaggaggtt ttaaagccat gacaatcagc
    23221 ggacgcactt tcatccaaaa gtttgaaagc tattgtcctc agtggctagc tgaagaagga
    23281 gacccagtcg gactgcatat cggaacattg gataagccaa tccaacgagt aatgatgaca
    23341 ttagacgttc gtccagaagt ggtcgaagaa gcaatcaaga aaaaaatcga tttgctgatt
    23401 gctaaacatc ctccaatctt tcgtcctgta aaacggctgg ttactgatca gccacaagaa
    23461 aaaatgtatg cagatttgtt gaaacatgat attgccgtct atgcagcaca cacgaacatg
    23521 gatattattt gggatggact gaatgactgg ttttgtgaac ttctcggtat tgaagtggag
    23581 agttatttag taaagactca tgagattcat tataaaaaac tggccgttta tgtacctgtg
    23641 gatcatgcac agaaaatgcg tgaagtatta gctgcagctg gtgctggtac acaaggtgat
    23701 tataccggta caagttttac ctcgattgga cacggtcgat ttactccgga agctggtgca
    23761 caaccagcga ttgggaaagt agggaagaca gaacaagttc aagaggcaaa agttgaggtt
    23821 attttgccgg aaactatcga aaaacaagtg attcaagcca tgcggtcagc tcatccttat
    23881 gaggaacctg cttatgatct tttcgctata gatgaaccag tcgaaatgtt tgggttagga
    23941 cgagtaggtg aattgccgca agaaatttct atagaggcat ttgtagaaca agtaaaagaa
    24001 gcgttccaac tagatggact gagaatagtc cagccaaaaa atgcaaaatc ctctgtgaag
    24061 cgcattgcga tttgcggtgg aagcggtgaa aaattttatc cgcaagcaat cgcccaaaga
    24121 gcagatgtct atatcacggg agatatctat tatcatacgg ctcatgatat gcaaagcgct
    24181 gggttgattg caattgatcc cggtcattat attgaatcgt tatgcaagca acgatttatc
    24241 gagaaatttg aatcttggaa acaagaagaa aattgggata ttaacttttt cgtttctgaa
    24301 acaaatacaa acccatttca atttaattaa aaggagagaa tactatgtac gaaaatcttt
    24361 tgccacgttt tttacgatat gtcaaaacag aaacacgctc cgatgcaaca agcaaaacga
    24421 ctccatcgac tcagacacaa gttgcttttg cgcaagaatt gaaaaaagaa ttagaagagt
    24481 taggcttagc tgatgttttc tataatgaag ataacggatt cgttatggga acattaccaa
    24541 gcaatgttga tcatgaagtg cgttccattg gatttatttc ccacatggat acagcagatt
    24601 ttaatgcagt aggagtcaat ccacaaatca ttgaaaacta tgatggcgag tctacgatcg
    24661 tactagatgc ggaagaacgc tatacattga atacaaaaga ttttccaaat ttgaaaaatt
    24721 atgaaggcca cacattgatc acaacagatg gcagtacatt actgggagca gatgacaaat
    24781 cagggattgc ggaaatcatg actgcaatgg agatcttgat caataatcct tctatccctc
    24841 acggtaccat caaagtggct tttggtcctg atgaagaaat cggtgtcgga gcggataaat
    24901 tcgatgttga gcaattcgat gtagattttg cttatacagt cgatggtggt cctttaggtg
    24961 agttgcagta tgaaacgttt agtgctgctc aagcaaatat tacgatccaa gggaaaaatg
    25021 tacatccagg gacggcaaaa gatacaatga tcaatgctct acagttagca atcgattttc
    25081 ataatcagct tccagccgat gaagttccag aaaaaacgga aggatacgaa ggtttcttcc
    25141 atttgatggc attggatggg aatcctgaag aagcttctat gagttatatc attcgcgatc
    25201 atgatcgtga aaaattcgaa gcacgcaaag cacaaatcac acatatccaa gaagagttga
    25261 atcagcgatt tgatcaagaa cgaatcaaag tagatatgta tgatcaatat tacaacatga
    25321 aagaaatcat tgaaaaagat atgagcatcg tggaattagc gaaacaagca atgatcgagc
    25381 ttggtatctc tccagtgatc gaaccagttc gtggaggaac agatggttca aaaatctctt
    25441 acatgggtat tccgacgcca aatctatttg ccggaggaga aaatatgcac ggacgattcg
    25501 aatttgtttc tttacaagta atggaaaaag caacagacgt gattgtaaaa attgcagaat
    25561 tgaacacaaa gagttaaaat cgaaaaagaa cagcttatga caaggcgttc gtcccaatga
    25621 tggaatgtca tctgtctaag gctaaagggc aggacaatgg aattggttgt cccgccctct
    25681 tttttgaaga gtaaatagga ggtaaagaat ggaaaagctg ctagtttttt ataacaaaag
    25741 ttcagggaaa gatgagggtg agaaattagc acgctggttt caagattatg taaaagaaaa
    25801 gcagccagaa ttatatgtag aactagctga aactggccca tcgattgatg atgcggaatt
    25861 agtcaagaaa gcagaagcca tagaagctga tactttagta attataggtg gagacggcac
    25921 gattcatcat atcgtccaag cgttccaaaa aacattatct caataccatg tcggattact
    25981 tccaggtgga actgtcaaca accttgcacg tgttcttgag attcccctca aaaaagaaga
    26041 agctgctgac gtgatcctgg aaggcaatgt acggaaaata gactatggtc aagtcaatga
    26101 tgaagtcatt atctctacac tgacaatcgg aatcttagca gatacagcag tttggatcag
    26161 tcaagaagaa aaacagcgtt acggctcgtg gatctttatc aagaagtttt ttaagttatt
    26221 gctgaagaag aaaaaatatc atttggattt agaaacagac gaggagcatt ggaaaggaaa
    26281 atcccaactg ttgacagtca cgatgtctaa ctccgtgggc ggttttacaa attttgacga
    26341 ctcagcgacc ctagatgatg gaaaatttca tgtaacgatc gtaccttcct tagatatttt
    26401 ccgatttatt ttttatttgc ctagaatcat gagaggcaaa ttctatacga tccccggcat
    26461 tacttatttc acagccaaac gcatgaaaat ccaagcgcag gaaaaatctg tgggcactcg
    26521 aacggatgga gacacgacag atgatcttcc agttgaatta tctgttgtat caaaaggatt
    26581 aacagtttat tcaccaaaga aagaagaaaa agcttagaaa tagtagttaa cagaaagttg
    26641 gaagaagagt aatggatgta ttgttacaca atcctttgtg gttttacctt ttcattgtga
    26701 atttttactt attttgtctg atggggtatg ataaacatca ggctagaact agcggctggc
    26761 gtgttcctga gaataatttg atatttgtca gcctgttagg cggtggtttg ggaggattga
    26821 tctctcgaaa agttttccat cataaaactc ggaaaaagaa gtttactata ggattcgctg
    26881 caggtgtact tgttgatttg attctcattt attttttcca ttaaggagca ggagatgtcg
    26941 tctaaaggaa cgaaaactaa aatcattctg aatctgcttt tgctgattgt gatttttgcc
    27001 attatttatt atttaatcaa tcaatcattt gcagatatct ttgccgaatt gctgtctacc
    27061 agtgttgttg ttttggcagc ggctgttata ttagggacag tttaccaaat cgctgaaggg
    27121 cgctcaatca aagaaattgc tgaacctttc gcgccgaatt ttacaacgaa agatgggttc
    27181 ttgacctcta gctatattgc gttttaccga atcgtttcgt ttggtacagg aacgctgatc
    27241 tcggagattt atttttataa caaaaaagga atgaagctat cccagggagc aggggtgaca
    27301 gcactccata tgattatgta caaactggca gtgatcactt atgcattagc tggcttggtc
    27361 atccaattct ctctttttta ttctaatggt ccatcaatga tctggtttat catcgctggt
    27421 atccttttga cgatcgtgat tgttgccttt ttattggcga tgtccgtcag cttgaatgtt
    27481 caaatatttt ttgtcagctt atcgaataag ttatttaaaa gtgagcgttt gcggaaatgg
    27541 gtggatacat gcaatcagca gatctactct ttaagggaaa cggtccaaac cattatccaa
    27601 gacaggactg cgctattgcg gatttatcta tggaatatgt taaaacttgt attttggtat
    27661 gtacttcctt atttgattct gattgaaaac catcctaaaa tagattttct actgaccatt
    27721 tcgtttatca gctttgcagt agtgctttca ggggttattc ctacgcctgc tggtattggt
    27781 tcctttgaat ttgtctattt gttattattc agaccagttg ttggaacagt tgacgcagtt
    27841 tcttcgcttt tactgtatcg tttcagttcc tttatcttgc catttctcta tggatttgtg
    27901 tatgtgattg ctgaacggcg aagagtgatc aaagaagaaa taatagaagt aaaaaaagag
    27961 aataaagaaa atcaagggta ggtcttttat aagatctacc ctttttatca aatcaaagaa
    28021 aaacgttatg gataagataa ttttacgttt gtttggctct ttttttatat acatacaatc
    28081 aattttgttt ttttaagatt tatataagtc gagaaaaaaa gtcaaaaaac tatgtgtata
    28141 ttttgaccaa aattgaccaa agcgatataa tagtttttgt aaagatcaaa gaaataagtg
    28201 atcttacctt taattaggag ggtatacctg tatgaatatt gagaaaatga ctacgacgct
    28261 tcaagaagcg attgctgaag ctcaacagat tgcggtcact cgaaaacatc aagatatcga
    28321 cattgcccat gtatggaaaa tctttttaca atctaatcat tttggtcgaa atttctatac
    28381 ggatgccgga ttggatgtcg aatcttttga acatgaaatt gatcgtctac tggatgagta
    28441 tccagttgtt tctggtggaa acgtccaata tggacaaaac ctcagccaga atttctttcg
    28501 attattgaat gaagcagacc aaatacgtga aagcttcggc gacgagtttc tttcaacgga
    28561 agtcgttatt ttagcgttga tgaaattaaa aaattatccg ttaactgttt atttgaataa
    28621 aaacggatta tctgaaaaag aattgcgtaa aaacatcgaa gagatgagag gaggagaaag
    28681 agtgacctca caaaaccaag aagaacaata caaagcatta gaaaaatacg gcgttgattt
    28741 agtacagcaa gtcaaaagcg gaaaaatgga tccaatcatc ggtcgtgatg aagaaatccg
    28801 tgatgtcatc cgtattcttt cacgaaaaac aaaaaataat cctgttctga taggagaacc
    28861 cggtgtaggg aaaacggcaa tcgttgaagg gcttgctcaa cgtatcgtac gcaaagatgt
    28921 gcctgaaaat ttaaaagata aaacgatttt ttcattagat atgggtgcat tgatcgctgg
    28981 agcaaaattc cgtggtgaat ttgaagaaag actaaaagca gtcttgaaag aagtcaaaaa
    29041 aagtgatgga cgtatcctct tgttcatcga tgaaatccac aatatcgtag gggcaggaaa
    29101 aacagaagga agtatggatg ccggaaatct gttgaaacca atgcttgctc gcggtgaatt
    29161 acatttgatc ggtgccacaa ctttagatga gtaccgtcaa tatatggaaa aagacaaagc
    29221 gttggaacgt cgctttcaaa aggtattagt caaagaacca acagtggaag atacgatcag
    29281 tattctgcgt ggattaaaag aacgttttga gatccaccat ggtgttaaca tccatgacaa
    29341 cgcattagta gcagcagcta ctttatctga ccgttatatt actgatcggt tcttaccgga
    29401 caaagcaatc gatttgatcg atgaagccag tgcaacgatt cgggtagaaa tgaactctat
    29461 gccaacggaa ttagatcaag ttactagacg tttgatgcag ttggaaatcg aagaagctgc
    29521 actgaaaaaa gaatcagatg atgcaagtaa aaaacgcttg aaaaatttgc aagaagaatt
    29581 agctgaactt cgtgaagaag caaatgcgat gaagatgcag tgggaaacag aaaaagaaga
    29641 agtcaattct gtttcagcaa aacgtgctga aatcgataaa gcaaaacacg aattagaaga
    29701 tgcagaaaat aactatgatt tagaacgcgc tgctgtattg cgtcatggta cgatacctca
    29761 attagaaaaa gaattgaagg aattagaagc taaagcaaaa gacagcgaga ttaagatggt
    29821 tcaagagtcc gttaccgaaa atgagattgc tcaagtcgtt ggcagattaa cgggtatccc
    29881 cgtaacgaaa cttgtagaag gtgaacgaga aaaactaatt aaattgaatg aaacacttca
    29941 taaacgagtg attggtcaag atgaagcagt tgatgcggtc agtgacgcgg taattcgttc
    30001 acgggctgga ttgcaagacc caaatcgtcc tctaggttct ttccttttcc taggaccaac
    30061 tggtgtcgga aaaaccgaat tagcgaaagc gttagctgag aacttgtttg actcagaaga
    30121 tcatatggta cggatcgata tgagtgaata tatggaaaaa catgctgtgt ctcgtctagt
    30181 cggagctcct ccaggctatg taggttacga agaaggtggt caattaacgg aagctgtcag
    30241 acgaaatcct tatacaatcg tcttgttgga tgaaatcgaa aaagcgcatc ccgatgtctt
    30301 caatatcttg ttacaagtcc tagatgacgg acgcttgact gattcaaaag ggcgagtagt
    30361 agatttcaaa aatacggttc tgatcatgac cagcaatatc ggttcacagt tactgctgga
    30421 aggcgtgact gctgatggaa cgattccaga agcagtggct gaacaagtca acacgctatt
    30481 acgtggaaac ttcaaaccag aattcttgaa ccgaatcgac gacacgattt tatttacacc
    30541 attgagccta gacaacgtga aaggaatcgt tgacaaaatg gtggcgcaat tggcacaacg
    30601 tctggaacat caagaaatcc tgctgacaat cagtgatgaa gccaaaacgt ggattgctga
    30661 aaatgcttat gaaccagcat atggtgcaag accattgaaa cgatttatca ctaaggaagt
    30721 agaaacacca ctagctaaag aaatcgttgc aggacatgtt atgcctaaat caaaagtaac
    30781 gattacttta cttgatggac aacttcattt caaaacagaa gagctagaag aaatcgtcta
    30841 atcttccatt gagactataa aagaaaaaag gggtctgaca caactagtgt cagacctcct
    30901 tttttcaaat aaacagtgtt ccagaagtag tttcttcgaa aaataagccg aaatttcttg
    30961 aagctgcccg cttctgtcac aacctctttt attaggtatt tttcactttt tctaatttat
    31021 aactttcaaa gtaacgctca tcttttttac tttgagacca tgcaaacaat cccattaagg
    31081 cagcacctat cgtatttgtc agcaagtcgc ccatcgtatc catcaatgct gctcgtccga
    31141 caaacagtgt gccatcagaa gcagcaaaac gttgaagatt catgcctaag aactggtcgc
    31201 ataagaattc ccagaactcc caaaacacgc cgcaaagtcc agcaaacgca aagccgaata
    31261 agaggaacaa ccaaggactg gtttttgata tctcagcatc tttcatcaag taaccaatca
    31321 atccatagcc aagagctgtc aggaccattg gactcaccgc atgtaagatt ttgtcccaaa
    31381 aagagatgat actgatcaaa tgcatccctg tgcctaaaaa gacagagatg aacaagaaaa
    31441 accagtaaaa caaaacgatt gctgctggta ttttaagttt gaaaactttc aaaatgactt
    31501 gtggcatgaa aatcaatgca atccctaata aagattccac gataaatgtg attccttgag
    31561 aagtcgaata tttttgtgtg ataaattcat aaaaattata gcagacaacg aatacaccaa
    31621 aagcaagtaa agctatttgc acctttttga tttctttaat cactgaaagt ccctccatct
    31681 aaaaatcttt ttacttctga aagcaactca atttctgaag aaaccacttt tccgttcaat
    31741 ttgatcaaac ctattgtata taagttcagg tagtgaaatt gattttcagc tgtttcttgt
    31801 agtgcagcga tttttttcgg gtgggttccg ccttgctgtc gagtatcagt atataagccg
    31861 atcactggga tgcctttcgc ataagccaca ccgatttccg atgctacccc ggcatcgatc
    31921 gtcagcccat ctagtaaagc aatcattaaa tcactagcca acactttttc tgtgtcagca
    31981 agagcaatca ttcgactgtc tgcataagca gttttgtcat tgattgcttc attttcttga
    32041 ggaagataga tcgaaagctc agaagagagc tctctgattt ctttaactaa ctgttggtta
    32101 tataacaaat cacttttagc aaacattggt gcggcaaaat aaattttcat ctaaacatca
    32161 tcctttttat caaaaataat cgctaaagta agcttccatg tttggaatga tacgctctag
    32221 tgacttcagt gtttcttcat ctgtttctaa ccgttcgatt cttgccaaat aagaagcacg
    32281 gcgataattc atcatcaagc aagtcaatgt ttggatatcc agtcgaacgg attttcctat
    32341 aggttcattt aagacacgga cttgtccctg ttcatcccat gttaaagcga agacaccatt
    32401 attccaagga gcgacaggat cttcaatgat gaaatgaaaa gcatcagctg ttccaacaaa
    32461 cggaaaccgt tgaaggaatt cttttacatc aacgatccga gccataaaat atggttcgat
    32521 ctgctctttg atctgactat cttcaatcaa aaatgctaaa ggctcatttt tataaatatc
    32581 tccatgtacc cagtagatca tggaaaaatg tgcggaaata aagttccata agccatttcg
    32641 agcttcttga tcaagataga acatttcttt tacgtgaaat atttcttcag ctacccagta
    32701 gaacagaaca cctcttggtt catggttgct atcgtagtag acagcggctg tgcgctcatc
    32761 ttcattttcg aaacgccaat actcttccca atgaaaatca ctgcggaata aagcaccatg
    32821 gttttgttgg gcaaatttgt cataaacgga aaatacgtct ggatgatcaa ctggtaatcg
    32881 ctcaaccata ccacttactg ggatttgttt aggtagctgt gtatctctga ttttaaagga
    32941 aagtttatca gacatgattt cccatccttt acgccgatag taggggatac tgtaaggata
    33001 caaataagag atccattgct tattcttgcg catattatcc aatgcaacga taattagatc
    33061 ttgcatcaat ccatggttag catattctgg ataagttcca actccagtca caccacccat
    33121 tttataacgt gcaccgtgaa tattgacttc acaaggatag atagcgattt gagagatcag
    33181 tttattttcg tggaaccatc cgaacacttt cgatacttcc aaaatcggtt ttttggaacg
    33241 gataaaagct cgtttattct caaacccgct ttcctcgatg tctgcttcag tcacttggaa
    33301 cacataggaa agcaattcat tgaattgatc gatatgttct tgagtgaccg gttttaattc
    33361 aagattttct ctaaaatctt tttgatccat tttttcatct ccatttatta gcttgataac
    33421 agtatagcaa aattctacaa aaaaaacttc tatagctgac taattaaaaa ctctttaaat
    33481 tactgctttt cgttggtata atggaaagtg ctgattatat gaaaagagga gacgctatgg
    33541 atataaagaa aatgaaagaa cgccaagagc agattcgtaa tttttcgatc atcgctcata
    33601 tcgaccatgg gaaatctaca ttggccgatc gtattttaga aatgacgcac actgtgacgt
    33661 ctcgcgaaat gcaagatcag ctgttagact cgatggattt ggaacgtgaa cgtggaatca
    33721 cgattaaatt aaatgctgta gaactcaatt acacagcgaa aaatggagaa acatatatct
    33781 ttcacttgat tgacacccca gggcacgtcg acttcaccta tgaagtctct cgaagccttg
    33841 ctgcctgtga gggtgccgtt ttagtggtcg atgcagcaca aggaatcgaa gcacaaacat
    33901 tggccaatgt ttatttagcg ttagacaatg atttggagat cctgccagtc atcaataaaa
    33961 tcgacctacc cgcagcagat cccgaacgtg tccgtcaaga aatcgaagac gtaatcggaa
    34021 tcgatgctag tgaagctgta ttagccagtg ccaaatcagg gattgggatt gaagatattc
    34081 tcgagcagat cgtggaatat gttcctgcac caagtggaga cttagatgca ccgctaaaag
    34141 ctttgatctt tgactctatt tatgatagtt accgcggtgt tgttctaaat gtccggatca
    34201 cggacggtat ggtcaaaccc ggagacaaaa tcaaactgat gagtaatggc aaaacatttg
    34261 atgttacaga ggtcggggtc ttctcgccaa aagcagttgc tagagacttt ttgatggtcg
    34321 gtgatgtcgg ttatatcaca gcaagtatca aaactgtcca agatacacga gtaggggata
    34381 cagtaacgtt agcagacaat cctgccgctg aagctttacc aggttaccga aaaatgaatc
    34441 cgatggttta ttgtggtctt tatccaattg atacttctcg ttacaacgat ttgcgggaag
    34501 ctttagaaaa actgcaattg aatgatgctg ctttacaatt cgaaccagaa acctctcaag
    34561 cattaggttt tgggttccgc tgtggattcc tcggacttct tcatatggat gtcgtccaag
    34621 aacgcttgga acgggaattc aatctagaat tgatcacgac tgcaccatcc gttatttacc
    34681 atgttaataa aacggatggt acgactgcta cagtagataa cccggcagat tttcctgaac
    34741 ctgtaacgat tcaggatgta gaagaaccat tcgtcaaagc acagatcatg gtcccaaatg
    34801 attttgtcgg tgcagtaatg gaactttctc aacgaaaacg gggagaattc attacgatgg
    34861 attatttaga tgattaccga gtaaatgtcg tctataatat tccgctttct gaaattgtct
    34921 ttgacttttt cgacaaattg aaatcaagca cgaaaggcta tgcctcattg gattatgaaa
    34981 tgtctggtta tcaaaaaagc aagttagtga agatggacat cttgctgaat ggagaaaaag
    35041 tcgatgcatt aagctttatc gttcaccgag actttgccta tgaacgtgga aaagcaatcg
    35101 ttgaaaaact gaaaaaacta atcccacgcc aacaatttga ggtgccaatc caagcagcta
    35161 ttggacaaaa aatcgttgcc cgttcagaca tcaaagcctt gcgtaaaaac gtcttggcta
    35221 aatgttatgg tggagatgtt tctcgtaaac gtaaactctt agagaaacaa aaagaaggga
    35281 agaagcggat gaaacaaatc ggctccgtcg aagttccgca agaagccttc atggccgttc
    35341 tgaaaatgga tgagcaagat agtgtgaaat aacttataat caatcgaaaa acctattaaa
    35401 taaacctttt tgagtagatg caacactgag gattatctgt ataaaagatt ttaaactgtc
    35461 atttggttat cacggcgata ctaaaattaa caaaattgga atatttttct tgaagatttt
    35521 tagctgtcag tatatgatga aagcatctca taggaggtgc ttttttcatg gttgataatt
    35581 caagtggaag ggttcttaaa tctctaagaa aagagaaaaa actatctcaa aaaaagttag
    35641 ctgatttagc aggtatttca caaagtactt tagttaaata tgaaaaaggg tcaagaaaaa
    35701 tccctaaaga cgtagataat actctaagca agattctgaa tatagaaact ctaataaaag
    35761 atgaggaaga taagattgag attctcatcg gtaaactaat tgcatacaga gatatgaata
    35821 agttacttaa caaagagtta gctgataata taggaattag tgaagtatta ctcagttatg
    35881 tgttaaatag aaagagaaat ccgtcaaaag aaatgcaaaa aaaaatcgat atttttctat
    35941 taagtaatga aaaagaaatt ttgaaggaga ttaatcgtga tagtgagata tttagtttat
    36001 caaaagacga taaaattgtt atgggaaaaa gaatacgaga agtccgaaag aaccgggaag
    36061 agacgcttga aaaatttggt aaaaacttta ccatctatac aggcaaaaat gttataagta
    36121 gatgggaaaa aggtattaat attccagata ttgaaaaact aatgaatatt gcctatcttg
    36181 gtaaggtcac tgttccttac cttatgtacg gagaagatta taagaatata cttccaaaag
    36241 atgaaagagt tagcgatttt aaaaagataa attcttttag tatgggatta aggatgagga
    36301 aaattaggaa agattattat ctagaaaggg aggagtttgg aaagcttttt tctccttcaa
    36361 tttcaaaatg gtccatagat aggtatgaga atggtagaga tatacccaat actaatcgaa
    36421 t
//
LOCUS       DAZKLD010000010        36326 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.010, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000010 DAZKLD010000000
VERSION     DAZKLD010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 36326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36326
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.010"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            182..1198
                     /locus_tag="ACG8KR_000434"
     CDS             182..1198
                     /locus_tag="ACG8KR_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0199836.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            1339..2640
                     /locus_tag="ACG8KR_000435"
     CDS             1339..2640
                     /locus_tag="ACG8KR_000435"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016154 - pyrimidine-nucleoside
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0006213 - pyrimidine nucleoside metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HFQ0199837.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            2686..3348
                     /gene="deoC"
                     /locus_tag="ACG8KR_000436"
     CDS             2686..3348
                     /gene="deoC"
                     /locus_tag="ACG8KR_000436"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HFQ0199838.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            3369..3767
                     /locus_tag="ACG8KR_000437"
     CDS             3369..3767
                     /locus_tag="ACG8KR_000437"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HFQ0199839.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            3833..4915
                     /locus_tag="ACG8KR_000438"
     CDS             3833..4915
                     /locus_tag="ACG8KR_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HFQ0199840.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            5127..6689
                     /locus_tag="ACG8KR_000439"
     CDS             5127..6689
                     /locus_tag="ACG8KR_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0199841.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            6682..7815
                     /locus_tag="ACG8KR_000440"
     CDS             6682..7815
                     /locus_tag="ACG8KR_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0199842.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            7815..8771
                     /locus_tag="ACG8KR_000441"
     CDS             7815..8771
                     /locus_tag="ACG8KR_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0199843.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            8870..10036
                     /gene="deoB"
                     /locus_tag="ACG8KR_000442"
     CDS             8870..10036
                     /gene="deoB"
                     /locus_tag="ACG8KR_000442"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HFQ0199844.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            10060..10878
                     /locus_tag="ACG8KR_000443"
     CDS             10060..10878
                     /locus_tag="ACG8KR_000443"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HFQ0199845.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            10895..11602
                     /gene="deoD"
                     /locus_tag="ACG8KR_000444"
     CDS             10895..11602
                     /gene="deoD"
                     /locus_tag="ACG8KR_000444"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HFQ0199846.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
     gene            complement(11900..12586)
                     /gene="gpmA"
                     /locus_tag="ACG8KR_000445"
     CDS             complement(11900..12586)
                     /gene="gpmA"
                     /locus_tag="ACG8KR_000445"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HFQ0199847.1"
                     /translation="MPKLVFSRHGLSEWNALNQFTGWADVNLAPEGIEEAKEGGRKIK
                     EAGIEFDVAYTSVLTRAIKTCNLILEYSDQLWVPQIKSWRLNERHYGKLQGLNKKETA
                     EKYGDEQVHIWRRSYDTLPPLMEETDEGSAANDRRYAMLDKRDIPGGENLKVTLERAL
                     PFWQDEIAPALLDNKTVLVAAHGNSLRALAKHIEGISDEDIMDLEIPTGQPLVYELND
                     DLTVAKKYYL"
     gene            complement(12758..13339)
                     /locus_tag="ACG8KR_000446"
     CDS             complement(12758..13339)
                     /locus_tag="ACG8KR_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFQ0199848.1"
                     /translation="MKIIGYARTTINDVDLTTQVDQLSDFGCQEIFHETYEVEQNDST
                     AVHLESVIADMQQGDALVICQLHRLGKSTRQLTELTQLFKDRGLHLISLDEGIDTREM
                     GKIYFQLMENLANMECALIKERTLVGLDEARKKGKIGGRPKIDAKTIKKIRRLYYEKK
                     ETIQFISSKCGVSVGTCYKYINLPEKEIEQLCI"
     gene            complement(13472..14155)
                     /gene="rpiA"
                     /locus_tag="ACG8KR_000447"
     CDS             complement(13472..14155)
                     /gene="rpiA"
                     /locus_tag="ACG8KR_000447"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HFQ0199849.1"
                     /translation="MNLKQLAGIEAAKFVKDGMIVGLGTGSTAYYMVEEIGRRMREEG
                     LRITGVTTSNATKEQAEKLGIPLKSIDEVPVVDLTIDGADEISADFQGIKGGGAALLF
                     EKIVATYSKETIWIVDSSKLVDKLGKFPLPVEVIPYGSQQLLHIFEEKRFQPVLRTDE
                     NGEVLTTDGGHYIIDLHLEVIEQPESLATYLDELVGVVEHGLFLNMVSKVVVGSEKGV
                     EILDAIRRK"
     gene            14474..14887
                     /gene="rpsL"
                     /locus_tag="ACG8KR_000448"
     CDS             14474..14887
                     /gene="rpsL"
                     /locus_tag="ACG8KR_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HFQ0199850.1"
                     /translation="MPTINQLVRKPRKSKVEKSNSPALNKGYNSFKKTQTNVNSPQKR
                     GVATRVGTMTPKKPNSALRKYARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQSRSKYGTKKPKA"
     gene            14971..15441
                     /gene="rpsG"
                     /locus_tag="ACG8KR_000449"
     CDS             14971..15441
                     /gene="rpsG"
                     /locus_tag="ACG8KR_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HFQ0199851.1"
                     /translation="MPRKGPVAKRDVLPDPIYNSKLVTRLINRVMVDGKRGIAANIIY
                     NAFDLIKESTGNDPLEVFEQAMKNVQPVLEVKARRVGGSNYQVPVEVRPERRVTLGLR
                     WVVNYARLRGEHTMEERLAKEIMDAANNTGASVKKREDTHKMADANRAFAHYRW"
     gene            15535..17622
                     /gene="fusA"
                     /locus_tag="ACG8KR_000450"
     CDS             15535..17622
                     /gene="fusA"
                     /locus_tag="ACG8KR_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HFQ0199852.1"
                     /translation="MTREFSLQNTRNIGIMAHIDAGKTTATERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWKGHRINIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGAEDDFTGIIDLVTMKAEMYTNDLGTEIEETEIPEEYRELAEEWREKLVEAV
                     AETDEELTLKYLEGEEITEAELKEGIRRATVNVEFYPVLCGSAFKNKGVQLLLDAVLD
                     YLPSPLDIPAIKGIDPKTDEEVERPADDSAPFSALAFKVMTDPFVGRLTFFRVYSGVL
                     QSGSYVQNATKGKRERVGRILQMHANSRSEISEVYAGDIAAAVGLKDTTTGDTLCDEK
                     SLVILESMEFPEPVIQVAIEPKSKADQDKMGVALQKLSEEDPTFRAETNVETGETIIA
                     GMGELHLDIIVDRMRREFKVEANVGAPQVSYRETFRAGTQAEGKFVRQSGGKGQYGHV
                     WIEFTPNEEGAGFEFENAIVGGVVPREYIPAVETGLKDAMENGVLAGYPLVDIKAKLY
                     DGSYHDVDSNETAFRVAASMALRAAAKKANPVILEPIMAVEVVIPEDYLGDVMGHVTA
                     RRGRVEGMEARAGGQQVVRAMVPLAEMFGYATTLRSATQGRGTFTMTFDHYEDVPKSV
                     QEEIIKKNGGKAE"
     gene            17794..18981
                     /gene="tuf"
                     /locus_tag="ACG8KR_000451"
     CDS             17794..18981
                     /gene="tuf"
                     /locus_tag="ACG8KR_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HFQ0199853.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLSKKNGGQA
                     MAYDQIDGAPEERERGITISTAHVEYETDTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKVDMVDDEELLELVEMEVRDLLTE
                     YEFPGDDVPVVAGSALKALEGDASYEEKILELMAAVDEYIPTPERDNDKPFMMPVEDV
                     FSITGRGTVATGRVERGQVRVGDEVEVVGIAEETSKTTVTGVEMFRKLLDYAEAGDNI
                     GALLRGVAREDIQRGQVLAKPGTITPHTKFSAEVYVLTKEEGGRHTPFFTNYRPQFYF
                     RTTDVTGVVELPEGTEMVMPGDNVTMEVELIHPIAIENGTKFSIREGGRTVGAGVVTE
                     IKA"
     gene            19388..19696
                     /gene="rpsJ"
                     /locus_tag="ACG8KR_000452"
     CDS             19388..19696
                     /gene="rpsJ"
                     /locus_tag="ACG8KR_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HFQ0199854.1"
                     /translation="MAKQKIRIRLKAYEHRILDQSADKIVETAKRTGASVSGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTPKTVDALMKLDLPSGVNIEIKL
                     "
     gene            19727..20356
                     /gene="rplC"
                     /locus_tag="ACG8KR_000453"
     CDS             19727..20356
                     /gene="rplC"
                     /locus_tag="ACG8KR_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HFQ0199855.1"
                     /translation="MTKGILGKKVGMTQIFTESGELIPVTVVEATPNVVLQVKTMETD
                     GYEAIQVGYQDMREVLSNKPAKGHVAKANTAPKRFIREFKNVELGEYEVGKEITVDVF
                     QAGDIIDVTGTTKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRL
                     AGRMGGNRVTIQNLEVVRVDAERNVILIKGNVPGAKKSLITIKSAVKAK"
     gene            20384..21007
                     /gene="rplD"
                     /locus_tag="ACG8KR_000454"
     CDS             20384..21007
                     /gene="rplD"
                     /locus_tag="ACG8KR_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HFQ0199856.1"
                     /translation="MPNVALFKQDGSQNGEITLNEEIFGIEPNESVVYDAIVMQRASL
                     RQGTHAVKNRSAVRGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYSYKLP
                     KKVRRLAMKSVLSEKVAENNLVAIEGLNFDAPKTKEFKQVLANLSIDSKVLVVLEKGN
                     DFAALSARNLPNVSVVTSDNVSVLDVVSNTKVLATQTALTQIEEVLA"
     gene            21007..21297
                     /gene="rplW"
                     /locus_tag="ACG8KR_000455"
     CDS             21007..21297
                     /gene="rplW"
                     /locus_tag="ACG8KR_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HFQ0199857.1"
                     /translation="MNLLDVIKRPVITEKSMLAMDDKKYTFEVDTRANKTLVKQAVEA
                     AFDVKVKNVNIVNVRAKFKRMGKYAGYTKKRRKAIVTLTEDSKEIQLFEAAE"
     gene            21336..22169
                     /gene="rplB"
                     /locus_tag="ACG8KR_000456"
     CDS             21336..22169
                     /gene="rplB"
                     /locus_tag="ACG8KR_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HFQ0199858.1"
                     /translation="MAIKKYKPTSNGRRNMTGSDFAEITTSTPEKSLLQPLKNHAGRN
                     NNGRITVRHQGGGHKRQYRVIDFKRNKDNVVATVQTIEYDPNRSANIALVHYEDGVKA
                     YILAPKGLEVGMKLVSGPEADIKVGNALPLENIPVGTVIHNIEMKPGKGGQLIRSAGT
                     SAQVLGKEGKYVLIRLNSGEVRMILAACRATIGSVGNEQHELINIGKAGRSRWMRKRP
                     TVRGSVMNPNDHPHGGGEGKAPIGRKAPVSPWGQPALGYKTRNKKAKSDKLIVRRRKN
                     K"
     gene            22212..22490
                     /gene="rpsS"
                     /locus_tag="ACG8KR_000457"
     CDS             22212..22490
                     /gene="rpsS"
                     /locus_tag="ACG8KR_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HFQ0199859.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQAGVEKKKVIKTWSRRSTVFPSF
                     VGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYRGHAADDKKTKR"
     gene            22513..22860
                     /gene="rplV"
                     /locus_tag="ACG8KR_000458"
     CDS             22513..22860
                     /gene="rplV"
                     /locus_tag="ACG8KR_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HFQ0199860.1"
                     /translation="MAEQITSAKATAKTVRTSPRKARLVIDLIRGKSVADAISILKFT
                     PNKSAGIIEKVLMSAVANAENNFDLDVENLVVSEAFVNEGPTMKRFRPRAKGSASPIN
                     KRTSHITVVVSEK"
     gene            22874..23530
                     /gene="rpsC"
                     /locus_tag="ACG8KR_000459"
     CDS             22874..23530
                     /gene="rpsC"
                     /locus_tag="ACG8KR_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HFQ0199861.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYAEFLHEDLRIRKFIST
                     KLADAAVSTVEIERATGRVNISIHTAKPGMVIGKGGSEVESLRKELNKLTGKRIHINI
                     VEIKKPDLDAKLVGEGIARQLENRVAFRRAQKQAIQRSMRAGAQGIKTQVSGRLNGAD
                     IARSEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEILPTKKNTEKGGK
                     "
     gene            23533..23967
                     /gene="rplP"
                     /locus_tag="ACG8KR_000460"
     CDS             23533..23967
                     /gene="rplP"
                     /locus_tag="ACG8KR_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011375443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HFQ0199862.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQAVDSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKI
                     MFEIAGVPEEVAREALRLASHKLPMKTKIVKREEMGGESNEG"
     gene            23957..24145
                     /gene="rpmC"
                     /locus_tag="ACG8KR_000461"
     CDS             23957..24145
                     /gene="rpmC"
                     /locus_tag="ACG8KR_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HFQ0199863.1"
                     /translation="MKVKEIRELTTAEMLDQEKQLKEELFNLRFQLATGQLENTARIK
                     EVRKSIARIKTVLREQAK"
     gene            24170..24436
                     /gene="rpsQ"
                     /locus_tag="ACG8KR_000462"
     CDS             24170..24436
                     /gene="rpsQ"
                     /locus_tag="ACG8KR_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HFQ0199864.1"
                     /translation="MTEGRNQRKVYQGRVVSDKMDKTITVAVETKKNHPIYGKRMNYS
                     KKYKVHDENNTAKVGDIVRIMETRPLSATKRFRLLEVVEEAVII"
     gene            24494..24862
                     /gene="rplN"
                     /locus_tag="ACG8KR_000463"
     CDS             24494..24862
                     /gene="rplN"
                     /locus_tag="ACG8KR_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HFQ0199865.1"
                     /translation="MIQAESRLKVADNSGAREILTIKVLGGSGRKTANIGDVIVATVK
                     QATPGGVVKKGDVVKAVIVRTKSGARRTDGSYIKFDENAAVIIRDDKSPRGTRIFGPV
                     ARELRENNFMKIVSLAPEVL"
     gene            24899..25207
                     /gene="rplX"
                     /locus_tag="ACG8KR_000464"
     CDS             24899..25207
                     /gene="rplX"
                     /locus_tag="ACG8KR_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HFQ0199866.1"
                     /translation="MFVKKGDKVKVITGKDKNKEGVVLAAFPKKDKVIVEGVNVVKKH
                     QKPSQAAPQGGIVEMEAPIHVSNVMVVDSTGVAGRVGYKEVDGKKVRVSKKTGEVLDK
                     "
     gene            25235..25774
                     /gene="rplE"
                     /locus_tag="ACG8KR_000465"
     CDS             25235..25774
                     /gene="rplE"
                     /locus_tag="ACG8KR_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HFQ0199867.1"
                     /translation="MNRLKEKYLKEVTPSLMEKFNYSSVMQTPKVEKIVINMGVGDAV
                     SNAKNLDKAVEELTLITGQKPMITKAKKSIAGFRLREGMPIGAKVTLRGERMYEFLDK
                     LVSVSLPRVRDFHGVSKKAFDGRGNYTLGIKEQLIFPEVDYDLVDKVRGMDIVIVTTA
                     NSDEESRELLTQLGMPFQK"
     gene            25793..25978
                     /locus_tag="ACG8KR_000466"
     CDS             25793..25978
                     /locus_tag="ACG8KR_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HFQ0199868.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCERCGRPHSVYRKFHLCRICF
                     RELAYKGQIPGVKKASW"
     gene            26014..26412
                     /gene="rpsH"
                     /locus_tag="ACG8KR_000467"
     CDS             26014..26412
                     /gene="rpsH"
                     /locus_tag="ACG8KR_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010742763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HFQ0199869.1"
                     /translation="MVMTDPIADFLTRIRNANMVKHESLEVPASKIKRDIAEILKREG
                     FVRDVEYIEDDKQGVIRVFLKFGKNGERVITNLKRISKPGLRAYVKADEVPKVLNGLG
                     IAIISTSEGVVTDKEAREKNIGGEVIAYVW"
     gene            26444..26980
                     /gene="rplF"
                     /locus_tag="ACG8KR_000468"
     CDS             26444..26980
                     /gene="rplF"
                     /locus_tag="ACG8KR_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HFQ0199870.1"
                     /translation="MSRIGNKVVVIPEGVTVTQDGNNVTVKGPKGELTRTFSADIKMN
                     IEGNEVTFTRPNDSKEMKTIHGTTRANFNNMVVGVSEGFQKALELIGVGYRAQLQGSK
                     LVLNVGYSHPVEITPPAGVTVEVPANTQVIVKGANKEEVGELAANIRGTRPPEPYKGK
                     GIRYVGEYVRRKEGKTGK"
     gene            27141..27497
                     /gene="rplR"
                     /locus_tag="ACG8KR_000469"
     CDS             27141..27497
                     /gene="rplR"
                     /locus_tag="ACG8KR_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014125626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HFQ0199871.1"
                     /translation="MITKPDKNKTRQKRHRRVRNNISGTAERPRLNVFRSNKNIYAQI
                     IDDVAGVTLASASTLDKEISGGTKTEAAQAVGKLVAERAAEKGIKVVVFDRGGYLYHG
                     RVQALAEAARENGLEF"
     gene            27518..28018
                     /gene="rpsE"
                     /locus_tag="ACG8KR_000470"
     CDS             27518..28018
                     /gene="rpsE"
                     /locus_tag="ACG8KR_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HFQ0199872.1"
                     /translation="MVYIDPKHLELEDRVVAINRVTKVVKGGRRLRFAALVVVGDKNG
                     HVGFGTGKAQEVPEAIRKAIEDAKKNLVEVPMVGSTIPHEVIGAFSGGRILMKPAVEG
                     SGVAAGGPVRAVLELAGVADITSKSLGSNTPINVVRATVEGLKQLKRAEEVAELRGKS
                     VEELIG"
     gene            28033..28212
                     /gene="rpmD"
                     /locus_tag="ACG8KR_000471"
     CDS             28033..28212
                     /gene="rpmD"
                     /locus_tag="ACG8KR_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HFQ0199873.1"
                     /translation="MAELKVTLKRSIIGRPQNQRDTVKALGLTKINSSVVKPSNEAIK
                     GMINTVSHLVDVEEI"
     gene            28256..28696
                     /gene="rplO"
                     /locus_tag="ACG8KR_000472"
     CDS             28256..28696
                     /gene="rplO"
                     /locus_tag="ACG8KR_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005592004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HFQ0199874.1"
                     /translation="MKLHELKPAEGSRQVRNRVGRGTSSGNGKTAGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNVNRKDYAVINLDVLNRFEDGAEVTPVALVEAGIV
                     KNEKAGIKVLANGELNKKLTVKAAKFSKAAQEAIEAAGGSIEVI"
     gene            28696..29991
                     /gene="secY"
                     /locus_tag="ACG8KR_000473"
     CDS             28696..29991
                     /gene="secY"
                     /locus_tag="ACG8KR_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HFQ0199875.1"
                     /translation="MFRLLKDAFKVKDIRSKILFTVFVLFVFRLGAHITVPGVNAASL
                     QNLSSLPFFNMLNLVSGSAMQNFSIFSMGVSPYITASIVIQLLQMDIVPRFVEWSKQG
                     EVGRKKLNQATRYLTLVLAFIQSVGITAGFNQYAQLGFVNNPNVATYVTIGLILTAGT
                     MFVTWLGEQITEKGIGNGVSMIIFAGIISRLPESIREIYEDYFINIDPSDIWKSAIFV
                     AILLVAVLLIVTFVTFFQQAERKIPIQYTKRVAGAPTSSYLPLKVNAAGVIPVIFASS
                     FIATPNAVLQALSGRFGSENWYKVMMEIFNYNTAPGATIYTILIVAFTFFYAFVQVNP
                     EKLAENLQKQGSYIPSVRPGKGTEEYVSRLLMRLSTVGAVFLGLVALLPIIAQMVWDL
                     PQSIGLGGTSLLIVIGVALETAKQLEGLMLKRKYVGFIE"
     gene            30053..30700
                     /locus_tag="ACG8KR_000474"
     CDS             30053..30700
                     /locus_tag="ACG8KR_000474"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HFQ0199876.1"
                     /translation="MNLILMGLPGAGKGTQAEKIIDAYGIPHISTGDMFRAAMQNETA
                     LGLEAKSYMDKGALVPDEVTNGIVKERLAEPDTEKGFLLDGFPRTLDQAKALDAMLKD
                     LNKKIDAVIDIHVGEEILVERLAGRFICSNCGATYHKIFNPTKVEDTCDRCGGHEFYQ
                     REDDKPETVKNRLAINIKNSEPILAYYKEQGLLNTIDGAREIDAVFADVQKIIEK"
     gene            30891..31109
                     /gene="infA"
                     /locus_tag="ACG8KR_000475"
     CDS             30891..31109
                     /gene="infA"
                     /locus_tag="ACG8KR_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HFQ0199877.1"
                     /translation="MAKEDMIEVEGTVVETLPNAMFKVELENGHQVLATVSGKIRMHY
                     IRILPGDKVTVELSPYDLNRGRITYRFK"
     gene            31142..31258
                     /gene="rpmJ"
                     /locus_tag="ACG8KR_000476"
     CDS             31142..31258
                     /gene="rpmJ"
                     /locus_tag="ACG8KR_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HFQ0199878.1"
                     /translation="MKVRPSVKPMCEHCKVIRRKGRVMVICPANPKHKQRQG"
     gene            31276..31641
                     /gene="rpsM"
                     /locus_tag="ACG8KR_000477"
     CDS             31276..31641
                     /gene="rpsM"
                     /locus_tag="ACG8KR_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HFQ0199879.1"
                     /translation="MARIAGVDIPRDKRVVVSLTYIYGIGNTTAKKILADAGVSEDVR
                     VRELTNEQTDAIRAEVDKLKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGPARAIAGKKK"
     gene            31669..32058
                     /gene="rpsK"
                     /locus_tag="ACG8KR_000478"
     CDS             31669..32058
                     /gene="rpsK"
                     /locus_tag="ACG8KR_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HFQ0199880.1"
                     /translation="MAAKKVNRKRRVKKNIEAGIAHIHSTFNNTIIMITDTHGNALAW
                     SSAGSLGFKGSKKSTPFAAQMAAETATKAAMEHGLKTVEVTVKGPGSGREAAIRSLQA
                     TGLEVTAIRDVTPVPHNGARPPKRRRV"
     gene            32138..33076
                     /locus_tag="ACG8KR_000479"
     CDS             32138..33076
                     /locus_tag="ACG8KR_000479"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010774253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HFQ0199881.1"
                     /translation="MIEFEKPRIAKIDEEKDYGKFIVEPLERGYGTTLGNSLRRILLS
                     SLPGAAITNIQIDGVLHEFSTIKGVREDVTQIILNIKGLALKMYGQEEKTLEIDITGP
                     ATVTAGDIIVDSDVEILNKDMYICSVSEGATFHARLTVKPGRGYVQADENKKEDMPIG
                     VLPVDSIYTPVRRVNYQVENTRVGHREDFDKLTMEIWTDGSIEPLEAMSLSAKIMTEH
                     LDIFVNLTDEAKNAEIMVEKEETQKEKMLEMTIEELDLSVRSYNCLKRAGINTVQELT
                     NKSEPEMIKVRNLGRKSLEEVKAKLHDLGLGLRKED"
     gene            33104..33484
                     /gene="rplQ"
                     /locus_tag="ACG8KR_000480"
     CDS             33104..33484
                     /gene="rplQ"
                     /locus_tag="ACG8KR_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HFQ0199882.1"
                     /translation="MSYRKLGRTSSQRKAMLRDLTTDLLINERIVTTEARAKEVRSTA
                     EKMITLGKRGDLHARRQAAAFVRNEVASVREENEDIVIESALQKLFNDIAPRYAERQG
                     GYTRILKTEPRRGDGAPMVVLELV"
     gene            33756..34595
                     /locus_tag="ACG8KR_000481"
     CDS             33756..34595
                     /locus_tag="ACG8KR_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HFQ0199883.1"
                     /translation="MEPIIELEKINYKYQPDDLRPALKDVSFTIDKGEWIAIIGHNGS
                     GKSTLAKTINGLLLPESGIVKVGNQILDEENIWTIRQMVGMVFQNPDNQFVGSTVEDD
                     VAFGLENQGIPREEMLVRVKDALEKVRMAEFASREPARLSGGQKQRVAIAGVVALRPD
                     IIILDEATSMLDPEGREEVISTIKKIKEESQLTVISITHDIDEAANANRILVMRQGEL
                     VREGTPKEIFSAGPELIDLGLDLPFPEKLKSALKERGVDVPSEYMTEERMVDWLWTSV
                     LNK"
     gene            34571..35440
                     /locus_tag="ACG8KR_000482"
     CDS             34571..35440
                     /locus_tag="ACG8KR_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HFQ0199884.1"
                     /translation="MDIRFEQVDFTYQPNTPFEQRALFDINMTIKENSYTALVGHTGS
                     GKSTLLQHLNALVKPTSGTVHIGERDIQPDTDNKNLKPIRKKVGIVFQFPEAQLFEET
                     VAKDIAFGPKNFGVSEEEALVLAKETLEQVGLDESYLERSPFELSGGQMRRVAIAGVL
                     AMRPEVLVLDEPTAGLDPQGRKEMMEMFWRLHKEQQITIVLVTHLMDDVANFADYVYV
                     LEKGRIVNSGEPQEVFQNIEWLKEKQLGVPTATEFAEELMAKGMNFATLPLTAEELAD
                     AIVHYAGGNAHDE"
     gene            35430..36227
                     /locus_tag="ACG8KR_000483"
     CDS             35430..36227
                     /locus_tag="ACG8KR_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HFQ0199885.1"
                     /translation="MMNKLIFGRYMPGDSLIHRLDPRAKLLASFYFIGIIFLANNWET
                     YLLLAFFTLLSVFLSKVDLKFFIRGIRPLIWLILFTVALQVLFTSGGETYWSWGVFHI
                     TEFGVMNGLFIFCRFVLIIFMSTLLTLTTPPLELSDAIEYLLRPLKAVRFPVHEISLM
                     LSIALRFVPTLMDETEKIMNAQRARGVDFGEGNLVQKMKAVIPLLIPLFVSSFNRAED
                     LATAMEARGYKGGEGRTKYRVLHWHNRDTMVIIAFVLLTVLLIFLRG"
BASE COUNT        11667 a         6650 c         8024 g         9985 t
ORIGIN      
        1 taggaacaga gtcgttgttt tttacgataa aataaataag atacagaact tgtatagaag
       61 gctgccaagt cggtagataa aggaggtttt tatcaaaaaa catactttcc ttttttctct
      121 attcttttct tgggggtttt ggtacactga tagggaattt agatagaagg gagccaatag
      181 gatgaagctg accatcagag atatcgcaga aatggcaggc gtatcagtaa caactgtctc
      241 acagattttg aataacaaag gaagcagatt tagtgataag acaagaaaaa aagtgttgtc
      301 gatcgtgaat gaacatcatt ataagcccga ttatttcgct tcaaatgtga tcaatcgaca
      361 ttcaaagacc atcggaatga tcgtccctga tgtcactgat ttcttcttct ccaaactgat
      421 cgaaggtgta gagagctatt tgaatccttt gggctatgtg atcatgttat gcaattctcg
      481 tcatagccaa gaaaatgaga tcaagtattt acaagagctt tcccaccgct cagtggacgg
      541 aatcttgctg gccacaccaa atattttacc tgagaaatat gcattagaca gcggttttta
      601 tcaaaaaatg ccaatcattt taatcgatcg cggattgaat cgtcgggata acggacggct
      661 tatcgtaaag gaatatgagg gcgcctatca agctgtttcg ctgtttattg aaaatggcca
      721 tacgaagatc ggcatgctga aggaaaccac tggatactat caattagaag aacgtttcaa
      781 cggatatcgt catgcgctga aagatcatgg cctgcctttt aatgggaaat ttgttgaaca
      841 tggagagttg actgtccaag gtggatatac tgcatcaaaa aaattactca aacataaaga
      901 tatcacggct attttttgcg ggaatgatgc catggcgatc ggatgctatc aggcgatcga
      961 tgaattgggc aaaaaaatcc cagaagatat ctcagtcata ggattcgatg gattaaagct
     1021 gtcagaatac atgattccgc aattgacgac agtgcagcag ccaagttttg atattggatt
     1081 ttatgctgca cgtttcctga tcgatacgat cgaatttcct caaagaaaag ttccgaacaa
     1141 agtgttcgaa acaaaattaa ttattcgtga gagcgtaaaa gtattgacaa agggctaact
     1201 cggcggtata ttgcttatgt aagcatttac aaaggctagt tagtgctttt tttattttta
     1261 gtaaaccagt ttactaaagt ggtttacctt tcctaaaaat aatgtataat accgaatgaa
     1321 gacaaggagg atcaggcaat gcgaatggtg gatctgattg aaaaaaagag ggatggaaac
     1381 gagctgtcga aagaggaaat cgagtacatc gtcacgaact atacgaatgg aaagattcca
     1441 gattatcaag tcagtgcatt attgatggca attttttatc aagacatgac aaatgaagaa
     1501 atcacgaact taacattggc gattgctaat tctggtgatg tcatcgattt gagttctctt
     1561 gaaggaataa aggtagataa gcattcgaca ggtggcgttg gtgacacgac cacattgatt
     1621 ttagcccccc tagtagctag tgtcggagtg acagtggcta aaatgtctgg acgcggtcta
     1681 ggttacacag gtggtacact agacaagcta gaagcaattc ccggatttca gatcgagctt
     1741 tcggatgaag aatttgtacg cattgttaat gaaagtaaag tagcggtaat tggtcaatca
     1801 ggaaacttgg caccggcgga caaaaaattg tatgccttgc gtgacgtcac agcgaccgtt
     1861 gattctcttc cgctgattgc cagttcgatt atgagtaaaa aaatcgcggc aggagcagat
     1921 gcaatcgttt tggatgtgac cactggagac ggtgctttca tgaagaacat cgaagatgca
     1981 agacgtctgg caaagaccat gacgagcatt gggaaactgg ctaaccgaga aacagtggcg
     2041 gttatctcag atatgtcaga acctcttggc gaagctatcg gaaatagtct agaagttgtc
     2101 gaggcaatcg aaacattaca aggaaatggt ccagaagatc tagtagaaat gtgctacgcc
     2161 ttaggaagcc aaatggttgt tcttgctggc aaagctaaga caaccgatga agcaagaacg
     2221 ttgcttcaag aagctctgga atcaggtaaa gcgttagcaa aattcaaaga aatgatccaa
     2281 aatcaagggg gagacccagc gatcgtggaa catccagaac gaattttgac tgcacgctac
     2341 acaatggagc ttcctgctaa acaaagcgga gttgtgtcaa aaatcgtggc aaatgaatta
     2401 ggaattgctg caatgatgct aggtgctggg cgtaaaacca aagaagaaga tattgatcac
     2461 gctgttggat tgaaactgca taaaaaaatt ggtgacactg tgacaaaagg tgagtcacta
     2521 ttgacgatct atagtaacga cgaagaaatc acttctgtca ttgaattatt atataaaaat
     2581 attgaaattg gtgaatcggc aatgaagcca acgttgattc atgacatcat cacagaataa
     2641 aggaaaaaag cggactttac cgttttaaag aaaaggggaa aagaaatgga actaaaccga
     2701 atgatcgacc atactatttt gaaagcagac gcatctaaag aagctgtgat gcaaattatc
     2761 gaagaagcaa aaaaatacca tttttattct gtctgcatca atccagcatg ggttgcgcta
     2821 gcgaaagaac aattacaagg tgagccagta gccgtatgta cagtaatcgg gtttcctcta
     2881 ggagcaaaca cacccgagac aaaagcattt gaaacaacag atgccatcaa taacggtgca
     2941 gatgaagtcg atatggtcat caatatcggt gctttgaaat caaaggatga tcaacaagtc
     3001 caaaaagata tcgaagcagt tgttgaagca gcaaaagatc gtgcgcttgt caaagttatt
     3061 attgaaacat cgttattaga tagagaagaa atcatccgag cttgcgaaat agcaaaagct
     3121 gcaggcgctg attttgtcaa aacatcaact ggtttctcaa caggcggagc aaaggtagag
     3181 gatgtccgtt tgatgcgtga aactgtagga ccagaaatgg gtgtcaaagc ttccggcggt
     3241 atccataacg aagaagaagc aatggcaatg atcgaagctg gtgctacaag aatcggaaca
     3301 agcgcaggag tagcaatcgt atctggttca actggtgaag gctattaatt ttttcaaagg
     3361 agaagctcat ggcagaactc aaacaagaat ggatcaacac agctattgaa gcgttagata
     3421 aagcctatgt tccttattcc cattttccgg taggcgcttg tctcgtaacg gaatcaggca
     3481 agatttatca aggcatcaac attgagaatg catcatttgg attaacgaat tgtgccgagc
     3541 gcacagcttt tttcaaagca gtatctgaag gagaaaaaac atttacgcat ctagtcgtag
     3601 cggggcacac ccccgatcct atctctcctt gtggtgcatg tcgacaagta atggcagaat
     3661 tctgttcccc tgatatgccg gttacgctag taggagacaa tggtgtgacg aaggcaacga
     3721 ccgttcgtgc actgctgcct tatgcattta ctgaaaaaga tctataactc atgttcattc
     3781 agtcagctga ctgttgaaaa ataaaacaat tattttagga ggctttacag aaatgaaaaa
     3841 agcaaaatta tttggtttag gtgctgtcgc actagcagca gggttatttt taggtgcatg
     3901 cggcaacaac ggctctactg attcatccgg tggcaaaagc tcatcagaca caacaactgc
     3961 agcattgatc acagacacag gtggtgtaga cgaccgttca ttcaaccagt cagcttggga
     4021 aggactagaa aaatggggga aagatcaagg actttcacga ggaaatgatg gcttccaata
     4081 tttccaatct tcaaatgaat cagattacat cccaaatatt gaccaagcat tgaatgctgg
     4141 attcaaaacg atttttggta ttggttataa actaaaacca gcgatccaag aacaagcaac
     4201 aaacaataca ggtacgaact ttgttattat cgatgatgtc atcgatggat tagataacgt
     4261 tgtatctgca actttcaaag acaacgaagc ttcttatctt gcaggtgttg cagcagctta
     4321 tacgactgaa acaaacgtag tcggctttat cggcggtgta aaaggtgaag taatcgatcg
     4381 ttttgatgct ggattcaaag ccggtgttga tgcaggtgcg aaagaattag gaaaagaaat
     4441 caaagtattg aatcaatatg ctggtgactt ctctgctcca gataaaggac gttcaattgc
     4501 tcaaggtatg tatgcacaaa acgcagatat catcttccat gcttcaggcg gtacaggtaa
     4561 cggtgtattc caagaagcaa aatcattgaa cgaatcaggc gacaaaaaag tttgggttat
     4621 cggtgttgac cgcgaccaat cagacgaagg cgaatacaca ttaaacggcg aaaagaaaaa
     4681 cttcacattg acatctacac tgaaagcagt tggaacagta gtagaagatt tggcacaaaa
     4741 atcagcggac ggtaaattcc ctggcggcga acacactgtt tatggattga aagaagacgg
     4801 tgttggtcta acagaaggac aactttctga cgaagcgaaa aaagcagttg atgaagcaaa
     4861 agaaaaaatc atttcaggtg acgtaaaagt tccagaaact cctgaagaaa actaaaacca
     4921 tttctatagc cttgttagac acatgtttga caaggctatg gtttgattag aacaagaatt
     4981 gttcatttat ccaagctgcc ttgtgacagt ttggataagg gaacaactcc ctgaagtaat
     5041 gtttgtttca aaagaagaac gctcactaag cgtgaatctt agtcaatcgt tgccctagat
     5101 agaaatcgtt aggatgtgaa aaaaaggtgg ttcaagaaaa tattgtcata gaaatgcaga
     5161 atattaccaa agaatttgga acattcaagg caaatgacaa catcaattta caagtaaagg
     5221 caggagaaat tcatgcgttg cttggagaaa acggtgctgg caaatctaca ttgatgaacg
     5281 tgctttccgg attattagag ccgacatcag ggaaaatttt gatgcgtggg aaagaagtaa
     5341 agatcacaag tccgaccaaa gccaaccaat tagggattgg gatggtccat cagcacttta
     5401 tgcttgttga tgcctttact gtaacagaaa acatcgtgtt gggaagcgag cctagtcgcg
     5461 cagggatgct tgaccataaa aaagcacgaa aagagatcca aaaagtttct gaacagtatg
     5521 gattgtcagt caacccagat gcttacgttc gtgatatttc agttggaatg gaacaacggg
     5581 tagaaatttt aaaaacactt taccgaggag cagatgtact gatttttgat gagccgacag
     5641 ctgtattgac ccctcaagaa attgatgaat taatcgtgat catgaaggaa ttagtcaaag
     5701 aaggcaagtc gatcattttg attacgcata agttagatga aatcaaagca gtagctgacc
     5761 gctgtacagt tatccgccgt ggaaaaggaa tcggtacagt caacgttaaa gacgtaacct
     5821 cacaacaatt agccgatatg atggttggaa gaacggtttc attcaaaacg atgaaaaaag
     5881 aagcgaagcc tcaagaagtc gttttgtcta ttgaaaatct agtggtgaaa gaaaatcgtg
     5941 gattagaagc cgtgaaaaac ttgaacttag aggttcgtgc tggcgaagta cttggtatcg
     6001 ctggaatcga tggaaacggg cagtcggagt tgatccaagc tttgactggt ttgcgaaagg
     6061 cagaaagcgg acatatcaag ctaaaagggg aagacatcac taataaaaaa ccccgaaaaa
     6121 tcactgaaca tggtgtagga cacgtgccag aagaccgtca taaatacggg ttagtcctag
     6181 atatgacatt atctgaaaat atcgcactac aaacctatca tcaaaaacct tacagtaaaa
     6241 acggcatgct gaattattca gtaatgaatg aacatgccag agaattgatc gaagaatatg
     6301 atgttcgaac aacgaatgaa cttgttcccg caaaagcttt atcaggcgga aatcagcaaa
     6361 aagcaattat cgctcgtgaa gtcgaccgag atcctgatct gttgattgtt gcaaatccaa
     6421 ctcgtggact ggatgtaggt gcgatcgaat ttattcataa acgtctgatc gaacaaaggg
     6481 acaaatacaa agcagtgtta ctgatcagtt tcgaattaga agaaatttta aatgtttcgg
     6541 atcgtattgc tgttatccat gaaggagaaa ttgtcggtat cgttgatccg aaagaaacat
     6601 ctgaaaatga attaggtcta ttgatggcgg gctatacctt agaagaagca cgaaaagagt
     6661 tagaaaaggg ggctggcgca catgaatgat cgcagcgaaa aattccgtaa tttattagtg
     6721 cctgtacttt cagttgtttt aggattgatc ttaggcgcga tattgatggc tgcttttggt
     6781 tttcagccca tccaaggtta ttcttctatg ctagatgcag cccttggttc gcaaagaggg
     6841 ataggtgaaa cgttaaggca agcaacgccg ttgattttta cagcattagg tttttctgta
     6901 gctaactcag ctgggttttt caatattggg ctttctggac aagcactttg cgggtggatc
     6961 gtcagtgtct gggtggcact tgcgtttcca gatctgccaa aagttgtttt gcttccgtta
     7021 tgcgtgatct taggtgcctt agctggtgcg atggcagcgg ctgttccagg tttacttcga
     7081 gcattttttg gaacaagtga agtgatcgta acaatcatga tgaactatat cttattatac
     7141 gtaagcacat ttgctttaca tgaagtgatg cctgaaagct atcgctcaag tttggactct
     7201 tccaatatga tcagtcaaaa tgcctcactg cggttaccat ggttaacaca agcttttggc
     7261 ggctctcgcg taaactcagg attattttta gcaatcatcg cgcttatttt cgtttggctg
     7321 ctgatgaaga aaacgacttt aggatttgaa atccgttctg ttggattgaa tccttttgct
     7381 tctgagtatg caggaatgag tagtaaacgt acgatcgttt tgtcgatggt catttctggc
     7441 gctttggcag ggcttggcgg tgtggttgaa ggattaggaa cgtatcaaaa cttctttgtt
     7501 caaacgactt cgctatctat tggatttgac ggaatggctg tttccttgct tggttctgga
     7561 tcagcaatcg gtattctttt atcagcgatc ttgttcagta ttttgaagat cggtggactt
     7621 ggcatgcaga caggagcagg tgttccgttt gagatcgtca atgtatcgat tgctttgatt
     7681 atcttctttg tcgggatcaa cttcctgatt cgtttcgtca tggctaaagt cttccaaggg
     7741 aaaaaacaag aagagatcgt tgcgacaata gaatcgaaac caacgaatca aaaccatgaa
     7801 gggggcgaac tataatgtcg actatcgaat taattgcctt gatccttact tctacactgg
     7861 tttattcaac accgctaatt ctaacgtcac taggcggaac tttttctgaa cgaagcggga
     7921 tcgtaaacgt agggctagag ggaatcatgg tcatgggtgc ttttagttcc atcgttttca
     7981 acttgacgat gagtccatca cttggttcta tgacaccatg gatcgctatt ttagtgggtg
     8041 gcgtcgtagg tatcctattt tctctgattc acgcagtagc aacagtcagt ttgcgtgctg
     8101 accatattat cagcgggaca gtaattaatc tgatggcacc tgctttaggt gttttcttga
     8161 tcaaagcatg gtatggtaaa ggtcaaactg ataacatcac gcagaatctg gggtattttt
     8221 ctttcccagg attagcagat attccggtca ttggaccaat tttctttaaa aatacattct
     8281 tacctgctta ttgtgctatt ttgattgctg tcctagcttg ggtgatcatc ttcaagacga
     8341 aattcggctt gcgcctccgt tcagttggtg aaaatcctca agctgccgac acattaggta
     8401 tcaatgtata tgctatgcgt tattcaggcg tactgatttc tggcttgtta ggcggtatgg
     8461 gcggtgctgt ttttgctcag tcgatttccg gtaacttttc agctggtacg atcgttggac
     8521 aagggttcat ctccatggct gctatgatct ttggtaagtg gaatccttta ggggcaatgg
     8581 gtgcctcatt gttctttggt tttgctcaaa gcttgagtat cattggcgca caattaccag
     8641 ttatttcttc gattccgcca gtattgctgc aaattgctcc atatttgctg acaatcattg
     8701 tgctggttgt tttcttaggg aaagcttctg gtccaaaagc aaacgggaag aactatatca
     8761 aatcaaaata atcttttagc gaataaaaag ggtgggttac gggaagtgtt tttcgtctcg
     8821 ccctttttgt tttaaaggca ataaaaataa aaagaaagaa ggaataatca tgtttaaacg
     8881 cgtacatctg atcgtcatgg attcagtagg aattggagaa gcaccagatg ccgataagtt
     8941 cggtgatgtt ggttctgata cattaggaca cattgcgaaa gaagcaggat tgaacgttcc
     9001 acatttagag cagcttggtt taggaacgat ccgtcctctt gaaggtgtgg aatcagtgaa
     9061 agatcatcaa ggctatgcga cgaaattaga agaagtttct gtagggaaag atacgatgac
     9121 aggccactgg gaaatcatgg gattgaatat caaaactcca tttcgtgtgt ttcctaatgg
     9181 attccctgat gatctgatca aacaaatcga agaattttct ggccgtaaag tcgtatgtaa
     9241 caaaccttat agcggaacag ctgttattga cgattacggt gaacaccaaa tgaaaacagg
     9301 cgatttaatt gtatatactt ctgccgatcc tgtccttcag atcgcggcac atgaagaaat
     9361 cattccttta gaagaattat atcggatttg ccaattcgtt cgtgatataa caaaagatga
     9421 tccgtatatg attggccgaa tcattgcacg tccgtacgtg ggtgaaccag gagacttcac
     9481 acgaacaagc aatcgccatg attatgcgtt gaatccattc ggacacacag tccttgattc
     9541 tttgaaagaa gcaggaaagg acgtgattgc tgttggaaag atcaacgata ttttcaacgg
     9601 acaaggaatc actgaatctg tccgcacgaa aagcaatatg gatggcgttg accagctgct
     9661 gaaagttatg aaaaacgatt tttcaggaat cagctttacc aatttagtag attttgatgc
     9721 attatacggt catcgtcggg atgtagtcgg ctatgcgcat gcgattgaag aatttgactt
     9781 acgaattcca gaattgcttg aagcgatgga agaagacgac ttgctaatga ttacagccga
     9841 tcacggaaac gatccgacgt ttactggtac cgatcatacg cgagaatatg taccgttatt
     9901 ggtttacagt aaaaaaatga aagaacaagg ggcattgcct caaggatatt attcagatat
     9961 ttcagcaacg attgctgaga actttagtgt ttcttcgact gaaaatggcg aaagcttctt
    10021 gaaattatta aaataatctt tttaaatagg agaaagcaaa tgaccaagtt aagcgagatg
    10081 ttacaagaaa cgacccagtt tatccttgat aaaggagtaa aagaaattga ttttggtttg
    10141 attctaggtt caggattagg tgaattagca gatgaaatcg aagatgctgt aatgatttct
    10201 tatgatgaga tcccgttctt tcctgtttcg acagtagttg ggcatgcagg aaagctagtt
    10261 tatgggtctc tggcaggcaa gaaagtgtta gccatgcaag ggagattcca ctactatgaa
    10321 ggtcattcca tgcaaacagt gacctatcca gtacgtgtga tggccggact aggggcccat
    10381 tcagtcattg tgactaatgc atgtggtggt gtcaacgaat catttgtacc aggagatttg
    10441 atgctgatta ccgatcatat caattttact ggtgaaaatc ctttgatcgg tccgaatgaa
    10501 gatgcaatgg gccctagatt tccagatatg agtcaagctt atacgcctga ctatcaagca
    10561 acagcaaaag cagcagcaga aaaactaggt ttggatctta aagaaggcgt atatatgggc
    10621 tattctggac caacttatga aacgcctgcg gagatccgaa tgtctcggac aataggagcc
    10681 gatgcagtag ggatgtcgac tgttccagaa gtcatcgtag cagctcacag cggattaaaa
    10741 gtgttaggaa tctcttgtat taccaactta gcagcaggta tgcaagcgaa tctgagccat
    10801 gaagaagttg tagaaacaac tcagagagtc aaacaatctt ttaaagcatt ggttaaagaa
    10861 acattgacat tattgtaata ggaggaacaa taaaatgagt gtgcatattg aagcgaaaga
    10921 aggcgagatc gccgataaga ttttacttcc gggagatcct ttgcgagcta aatatattgc
    10981 ggagactttt ttggaagatc cagtttgtta caatcaggta cgcggtatgt taggctttac
    11041 cggcaaatat aaaggagaac gtatttctgt tcaaggaaca ggaatgggaa tgccatcagc
    11101 caccatctat gcccatgaat tgatccagtc gtatggtgta aagaaattga ttcgagtagg
    11161 tacatgcggt gcattgtcaa aagacgtcca tgttcgtgat ttagtactag cacaaggtgc
    11221 agcgaccagt tcttcgatga tcgaaaaaaa tttccaagcc tttcattttc ctccaatcag
    11281 tgatttcaat ctattactga aagcatatga gatagctaaa gaaaagggct atacggttca
    11341 tgtaggaaat gttctttcgg aagattcttt ctacaaagat gatttgacag agacgttcca
    11401 attagctgaa cttggcgttt taggagtgga aatggaagca gcggctcttt attacttagg
    11461 cgcgaaatac catgtgcaga cattaagttt gatgaccgtt agcgaccatt tgatcaccgg
    11521 tgaagaaaca acagctgcag aacgtcaatc tacgttcaat gaaatgatcg aagtaggatt
    11581 ggaaacagcg atagcagaat aagaaggaaa tgaaaaagct gccctgcatt aagtaataag
    11641 aacatccaag caaaaatagc tcctcatgtt tttgacttgg ctggacttat tattgcaaat
    11701 gtaagctttt gaacaccgtt taccaagagg ttgtgaatct gccgtttaac tatgagtcac
    11761 aaggaacaat ttcaaaaaac agcttttcat gtttgatgaa tattgtgact tgtgaccgag
    11821 aagttggcag gagaaaaccg tttatacgaa aaaagggact gtgacaaatc gtgtcacagt
    11881 ccctttttta tagtgttttt tataggtagt attttttagc aactgttaag tcatcattta
    11941 attcataaac aagtggttga cctgttggaa tttcaagatc catgatgtct tcgtcagaga
    12001 taccttcgat atgtttagct agggcgcgta atgagttacc atgtgcagct actaaaacag
    12061 ttttgttgtc tagtaatgct ggagcaattt catcttgcca gaatggcaaa gcgcgttcta
    12121 atgtcacttt caagttttct ccaccaggaa tatcgcgttt gtctaacatt gcgtaacgac
    12181 ggtcatttgc tgcagaacct tcgtcagttt cttccattaa tggaggaaga gtatcgtaag
    12241 aacgacgcca gatgtgcact tgttcgtcgc cgtatttttc agctgtttct tttttgttca
    12301 aaccttgtaa tttaccatag tgacgttcgt ttaagcgcca tgatttgatt tgtggtaccc
    12361 aaagttgatc tgaatattct aagatcaagt tacatgtttt gattgcacga gttaaaacag
    12421 aagtgtaagc aacatcaaat tcgattccag cttctttgat tttgcgtccg ccttcttttg
    12481 cttcttcgat tccttctggt gctagattta catcagccca accagtaaat tggttcaatg
    12541 cattccattc actaagaccg tgacgagaaa aaactaattt tggcatgttt agctctcctt
    12601 ttaagatagg tttatatttc tgaaattttc agttctgttt tattgtacaa ttttttggaa
    12661 gagatttcca tcgaaaaatt agataagatt ctatgagaaa acaagtagag aatataaaaa
    12721 aacgaacagt gcccacgcaa ctgttcgttt ttaggtatta gatgcataat tgctcgattt
    12781 ctttttcagg taaattgata tatttataac atgttccaac tgaaacaccg cattttgaag
    12841 aaataaattg gatcgtttct tttttttcat aatagagacg tcggattttt ttgatcgttt
    12901 ttgcatcgat ttttggtctt ccgccgattt ttcctttttt acgagcttca tctaatccaa
    12961 ctaatgtacg ttctttgatc aatgcgcact ccatgtttgc taggttttcc attaattgga
    13021 aatagatttt tcccatttcg cgcgtatcga ttccttcatc aagactaatc aagtgcagcc
    13081 cgcgatcttt gaataattgc gtgagttctg ttaattgccg cgtagatttg cctaatcgat
    13141 gaagttggca aatgactagt gcatcgcctt gttgcatgtc agcgatcact gactccaaat
    13201 gaacagcagt agaatcattt tgttccactt catatgtctc atggaaaatt tcttgacaac
    13261 caaagtcaga taattgatca acctgagttg ttaaatcaac gtcatttatg gttgtgcgtg
    13321 cataaccaat aattttcatg gtgattcctt tctagcagtg attttctaac tggatataac
    13381 agtaaaaatg tagcataaag tacgtcaaaa agatagtttt ttcgcaaatt ttccgctgtc
    13441 tttttttatt ggatgatcaa gcagtttttc tttatttacg acggattgcg tcaagaatct
    13501 caacgccttt ttctgagccg acaactactt tagaaaccat gtttaggaaa agcccatgtt
    13561 ctactacccc aacaagttca tctaaatagg tagctaagct ttctggctgt tcgatgactt
    13621 ctagatgcag atcaatgatg taatgaccac catctgtggt taagacttct ccgttttcgt
    13681 ctgtacgcaa gacgggttgg aatctttttt cttcaaaaat atgaagtagc tgttgggagc
    13741 cataagggat cacttcaact gggagtggga acttcccaag tttatcgact aatttagaag
    13801 aatcaacgat ccaaatcgtt tcttttgagt acgtagcgac tattttctcg aaaagcaaag
    13861 cagcgccgcc gcctttgatc ccttgaaaat cagcactgat ttcatcagca ccgtctattg
    13921 tcaaatcgac tacggggact tcatcaatac tctttaacgg tattcctaat ttttccgctt
    13981 gttcttttgt cgcatttgaa gtagtaactc ctgtgatacg taaaccttct tcacgcatac
    14041 ggcgaccaat ctcttctacc atataatagg cggttgaacc ggtcccgaga cctacgatca
    14101 tcccgtcctt tacgaatttc gcggcttcaa tgccagctaa ttgttttaaa ttcatctttg
    14161 cgtccacctt tttttagttt gagcatcgtg ctttcctcac tattttagcg tgatttacgg
    14221 taaaaagata ccattctttt ttattccaat aaagcttttt caaaaaaaat tgcatctctt
    14281 gttgacatgt tttttcattc gtgatattct aacaaaggtg ctttatctac aggtttctgt
    14341 gaagaaacgt gctgactgtt tatcaaagca tatttgataa ggatgcagac attgcattta
    14401 ttttttatca caaaaaaaga accacctgga tgtgtggaac tagacgcaaa tcgtgggaag
    14461 gaggaaacaa ggaatgccta caattaacca attagtacgt aaacctcgta aatcaaaggt
    14521 ggaaaaatct aattcacctg ctttgaacaa aggatataac agctttaaga aaactcaaac
    14581 taacgtaaac tcacctcaaa aacgtggggt agcaactcgt gtgggtacaa tgacacctaa
    14641 aaaaccgaac tcagctttac gtaaatatgc acgtgttcgt ttgtcaaact taattgaagt
    14701 tactgcttat atcccaggga ttggtcataa cttacaagaa cacagtgttg tattattacg
    14761 cggtggtcgt gtaaaagact taccaggggt acgttaccat atcgttcgcg gtgctttaga
    14821 tactgctggt gttaacgacc gtaaacaaag ccgttctaaa tacggaacta aaaaacctaa
    14881 agcttaattt taattattaa acaaatgaaa cttgcttcta ctatatagaa gtaattacta
    14941 tgaacaatat ttcggaagga ggagttacgg atgccacgta aaggtcctgt tgcaaaacgt
    15001 gatgttttac cagatcctat ttataactca aaattagtaa ctcgcttgat caaccgtgta
    15061 atggttgacg gaaaacgcgg gattgcagct aacattatct ataatgcctt tgacttgatc
    15121 aaagaatcta caggtaacga tccattagaa gtgtttgaac aagcaatgaa aaacgttcaa
    15181 cctgttcttg aagttaaagc acgtcgtgtt gggggttcaa actaccaagt accagttgaa
    15241 gttcgccctg aacgccgcgt tactctaggt cttcgttggg tagtaaacta cgctcgcttg
    15301 cgcggtgaac acacaatgga agaacgctta gcaaaagaaa tcatggatgc tgcaaacaac
    15361 acaggtgctt ctgttaaaaa acgtgaagac actcataaaa tggctgatgc caaccgtgcg
    15421 tttgctcact atcgctggta agattctctc tgtttggtcg gcttttataa gtcgacctca
    15481 gatagtgtcc ttatttttag caactagata gtaaagagag gagaaacaag gagaatgacc
    15541 agagaatttt ctttacaaaa cactcgtaat atcggtatca tggcccacat cgatgctggt
    15601 aaaacgactg ctactgaacg tatcttgtac tatactggta aaatccataa aattggtgaa
    15661 actcatgaag gagcttcaca aatggactgg atggaacaag aacaagaacg tggtattacg
    15721 atcacttctg ctgctactac agcacaatgg aagggtcacc gaatcaacat catcgacaca
    15781 ccaggacacg tagacttcac agttgaagtt gaacgttcat tgcgtgtact tgatggtgct
    15841 gtaactgttc ttgatgcaca atcaggtgtt gaacctcaaa ctgaaacagt ttggcgtcaa
    15901 gcaacaactt atggtgtacc tcgtatcgta tttgcaaaca aaatggacaa gatcggtgct
    15961 gacttcttat attcagtatc aactttacat gaccgtttgc aagcaaacgc tcacccaatc
    16021 caattaccaa ttggtgcgga agacgacttc acaggaatta tcgaccttgt aacaatgaag
    16081 gctgaaatgt atacaaacga tcttggaaca gagatcgaag aaacagaaat tcctgaagaa
    16141 taccgcgaat tagctgaaga atggcgcgaa aaattggttg aagctgtagc tgaaactgat
    16201 gaagaactta ctttgaaata cttagaaggt gaagaaatca ctgaagctga gttaaaagaa
    16261 ggtatccgcc gcgcaacagt taacgtagaa ttctacccag tactatgtgg ttcagcattt
    16321 aagaacaaag gggttcaatt actattggac gctgttcttg attacttgcc atcaccgtta
    16381 gatatcccag ctatcaaagg gattgaccct aaaactgatg aagaagtaga acgtcctgca
    16441 gacgacagcg ctcctttctc agctttagca tttaaagtta tgactgaccc attcgtaggt
    16501 cgtttaactt tcttccgtgt gtactctggt gttttacaat caggttcata cgtacaaaac
    16561 gcaacaaaag gcaaacgtga acgtgttggt cgtatcctac agatgcatgc caactctcgt
    16621 tcagaaatct ctgaagttta cgctggagat atcgctgctg ctgttggttt gaaagacaca
    16681 actactgggg acacactatg tgatgaaaag agtctagtta ttcttgaatc catggaattc
    16741 ccagaaccag ttatccaagt cgctattgaa cctaaatcaa aagctgacca agataaaatg
    16801 ggtgtggctt tacaaaaact atctgaagaa gatcctactt tccgtgctga aacaaacgtg
    16861 gaaacaggcg aaacaatcat cgctggtatg ggtgaacttc acttagatat catcgttgac
    16921 cgtatgcgtc gtgaattcaa agttgaagct aacgtaggtg cgcctcaagt atcttaccgt
    16981 gaaactttcc gtgctggcac tcaagccgaa ggtaagtttg tacgtcagtc tggtggtaaa
    17041 ggtcaatacg gtcacgtatg gattgaattt acaccaaatg aagaaggtgc tggtttcgaa
    17101 tttgaaaatg cgatcgtcgg tggggttgtt cctcgtgaat acattccagc tgttgaaact
    17161 ggtttgaaag atgctatgga aaatggtgtc ttagctggct atccattagt agatatcaaa
    17221 gctaaacttt acgatggttc ataccatgat gtcgattcaa atgaaactgc cttccgtgtt
    17281 gctgcttcta tggcattacg tgcagcagct aagaaagcta atccagtaat ccttgaacca
    17341 atcatggctg tagaagttgt tatccctgaa gattatttag gtgatgttat gggtcacgta
    17401 actgctcgtc gtggacgtgt tgaaggtatg gaagctcgcg ctggcggcca acaagttgtt
    17461 cgtgcgatgg ttccacttgc tgaaatgttt ggttatgcta caactcttcg ttctgctact
    17521 caaggtcgtg gtacatttac aatgacattt gaccactacg aagatgtacc aaaatcagta
    17581 caagaagaaa tcattaagaa aaacggcgga aaagctgaat aatttcttga aaccggatag
    17641 tcctgcaaag agattatcca aagagactat atttttaata tagtttcgtg taaaataatt
    17701 atcgatgata tgggcatgtg gtgtaccttg tgtacatcat atacctatca gacaaataaa
    17761 aaatatattg tatttttagg aggacatttt aaaatggcaa aagaaaaatt tgaccgttct
    17821 aaaccacacg ttaacattgg tactatcgga cacgttgacc atggtaaaac tacattaaca
    17881 gctgcaatca caactgtact atctaagaaa aacggcggcc aagctatggc ttacgatcaa
    17941 atcgatggtg ctccagaaga acgcgaacgc ggaatcacaa tctcaacagc tcacgttgag
    18001 tatgaaacag atacacgcca ctacgctcac gttgactgcc caggacacgc ggattacgtt
    18061 aaaaacatga tcactggtgc tgctcaaatg gacggagcta tcttggtagt ttctgctgct
    18121 gacggcccaa tgcctcaaac tcgtgaacac atcctattgt ctcgtcaagt tggtgttcct
    18181 tacatcgttg tattcttgaa caaagtagac atggttgatg acgaagaatt actagaatta
    18241 gttgaaatgg aagttcgtga cctattaaca gaatacgaat tccctggtga cgatgttcct
    18301 gtagttgctg gatcagcttt gaaagctcta gaaggcgacg cttcatacga agaaaaaatt
    18361 cttgaattaa tggctgcagt tgacgaatac atcccaactc cagaacgtga caacgacaaa
    18421 ccattcatga tgccagttga agacgtgttc tcaattactg gacgtggtac tgttgctaca
    18481 ggtcgtgttg aacgtggaca agttcgcgtt ggtgacgaag ttgaagttgt tggtattgct
    18541 gaagaaactt caaaaacaac agttactggt gttgaaatgt tccgtaaatt gttagactac
    18601 gctgaagctg gagacaacat tggtgcttta ctacgtggtg ttgcacgtga agacatccaa
    18661 cgtggacaag ttttagctaa accaggtaca atcacacctc atacaaaatt ctctgcagaa
    18721 gtatacgtgt tgacaaaaga agaaggtgga cgtcatactc cattcttcac taactaccgt
    18781 ccacaattct acttccgtac aactgacgta acaggtgttg ttgaattacc agaaggaact
    18841 gaaatggtta tgcctggcga caacgtaaca atggaagttg aattaatcca cccaatcgct
    18901 atcgaaaacg gtactaaatt ctcaatccgt gaaggcggac gtacagttgg tgccggtgtc
    18961 gttacagaaa tcaaagctta attcacttaa gttttaaagt aaagcaacat ttattcggac
    19021 gtaatcgctc ggaacgaagg tcttaggatc tttgttccga gcttttttat ttattgcagg
    19081 atcaggaaaa aagacaggag tattataaat agaagaaaca cgaaataaaa gagtcagtat
    19141 tttctaaatg aaactttatg aaaacaaaat gttttttaca agaaaaagcc ttgctttgtg
    19201 agtttatata tattataatg acaaaggtgc gtttacttta gtgaattgca cagacgtaat
    19261 cagaatgatt tcgtcggcta agaaacgaga ggttgcgaca cgcccggacg ctttgccacg
    19321 aacgagcgtg acggaaattt tcgtggagct atgtctactt ttcaaaatag gcgaaggagg
    19381 gaacaagatg gcaaaacaaa aaattcgtat ccgtttaaaa gcgtatgaac accgtatttt
    19441 agatcaatca gcggataaaa tcgtggaaac tgcaaaaaga actggagcta gcgtgtctgg
    19501 tccaattcca ttaccaactg aacgcagtct ttacacaatc atccgtgcga ctcataaata
    19561 caaagattca cgcgaacaat tcgaaatgcg tacacacaaa cgtcttatcg acattgtgaa
    19621 cccaacacca aaaactgttg atgctttgat gaagctagac ttaccatctg gtgtaaacat
    19681 cgaaatcaaa ttataaaact aaaacaaaaa taatggaggt gtactcatga ccaaaggaat
    19741 cttagggaaa aaagtgggaa tgacacaaat cttcactgaa tctggtgaat taattccagt
    19801 aactgttgta gaagctactc caaacgtagt actacaagtg aaaacaatgg aaacggatgg
    19861 ctacgaagct attcaagttg gttaccaaga tatgcgtgaa gttttatcaa acaaacctgc
    19921 gaaaggtcat gttgcaaaag caaacacggc tcctaagcgc ttcattcgtg aattcaaaaa
    19981 tgttgagcta ggagaatatg aagtaggaaa agaaattaca gtagatgttt tccaagcagg
    20041 agacattatt gatgtaacag gtactacgaa aggtaaagga ttccaagggg ttatcaaacg
    20101 ccacggacaa agccgcggac caatggctca cggttctcgt taccatcgtc gtcctgggtc
    20161 aatgggtcca gttgcaccta accgtgtatt taaaaacaaa cgtcttgctg gccgtatggg
    20221 tggcaaccgc gtaactatcc aaaacttgga agttgtacgt gtcgatgcag aaagaaatgt
    20281 tatcttgatc aaaggtaatg ttcctggagc taaaaaatct ttaattacta tcaaatcagc
    20341 tgtgaaagct aaataattgt aggaagaaag gaggaactaa ggaatgccga atgtagcatt
    20401 attcaaacaa gatggaagcc aaaacggtga aatcacactt aacgaagaaa tcttcggaat
    20461 tgaacctaat gaaagtgttg tttacgatgc aatcgtaatg caacgtgcgt cattaagaca
    20521 aggaacacac gcggttaaaa accgtagcgc tgttcgcggc ggcggacgta aaccatggcg
    20581 tcaaaaagga acaggtcgtg cacgtcaagg atctatccgc tcaccacaat ggcgtggagg
    20641 tggcgtagtc tttggaccaa caccacgttc ttacagctac aaacttccta aaaaagttcg
    20701 tcgtttagca atgaaatctg tattgtcaga aaaagttgct gaaaacaact tggtagcaat
    20761 cgaaggcttg aacttcgacg caccaaaaac aaaagaattc aaacaagttc ttgctaactt
    20821 atctatcgac tctaaagtat tggttgttct agaaaaaggt aacgactttg ctgctttatc
    20881 agcacgtaac ttaccaaacg tttctgtagt aacttctgat aatgtgagtg tgctagacgt
    20941 tgtatcaaat acaaaagtgt tagcaacaca aactgctctt actcaaattg aggaggtgct
    21001 tgcataatga acttactaga cgtaatcaaa cgcccagtga tcactgaaaa atcaatgctt
    21061 gccatggatg acaagaaata cacttttgaa gtggacactc gcgcaaacaa aactttagta
    21121 aaacaagcgg tagaagctgc ttttgacgtt aaagtcaaaa acgtgaacat cgtgaacgta
    21181 cgtgcgaaat tcaaacgtat gggcaaatat gcaggatata caaaaaaacg tcgcaaagcg
    21241 atcgttacat tgactgaaga ttcaaaagaa atccaattat tcgaagctgc tgaataagca
    21301 ctaatagata actcattaaa ttaggaggga aaaacgtggc gattaaaaag tacaaaccta
    21361 cctcaaatgg tcgtcgtaac atgacaggtt ctgattttgc tgaaatcaca acatcaacac
    21421 ctgaaaaatc attgttacaa ccattgaaaa accatgctgg acgtaacaac aacggccgca
    21481 tcactgtacg tcatcaaggt ggcggacaca aacgccaata tcgcgtgatc gacttcaaac
    21541 gtaacaaaga taacgttgtt gcaactgttc aaacgatcga atatgatcca aaccgttcag
    21601 ctaacatcgc gttagtacac tatgaagatg gagtgaaagc ttacatctta gcacctaaag
    21661 gactagaagt gggtatgaaa cttgtttcag gtccagaagc agatatcaaa gtcggaaatg
    21721 ctcttccatt agaaaacatt ccagttggta ctgttatcca caacattgaa atgaaacctg
    21781 gtaaaggtgg acaattgatc cgttctgctg gtacaagtgc tcaagtactt ggtaaagaag
    21841 gcaaatacgt attgatccgc ttgaactctg gtgaagttcg tatgatctta gctgcatgcc
    21901 gtgctacaat cggttctgta ggtaacgaac aacacgaatt aatcaacatc ggtaaagctg
    21961 gacgctctcg ttggatgcgt aaacgcccaa ctgtacgtgg tagcgtaatg aaccctaatg
    22021 atcacccaca cggtggtggt gaaggtaaag ctcctatcgg acgtaaagca ccagtttctc
    22081 cttggggtca accagctctt ggttacaaaa ctcgtaacaa aaaagctaaa tcagacaaac
    22141 ttattgttcg tcgtcgcaaa aataaataat tactttgacc atgtgtcgca cattttgaga
    22201 ggaggttcac catgggtcgt agtttaaaga aaggaccttt tgtcgatgag catttaatga
    22261 aaaaagttga agctcaagca ggggttgaaa agaaaaaagt tatcaaaact tggtcacgcc
    22321 gttcaacagt tttcccaagt tttgttggat ataccatcgc agtttatgat ggacgtaaac
    22381 atgtaccagt ttacatccaa gaagacatgg taggacacaa attaggtgaa tttgcaccta
    22441 caagaactta tcgtggacat gctgctgacg ataagaaaac gaaacgctaa tttgagggga
    22501 ggataaatcc aaatggcaga acaaatcaca tcagctaaag caactgcaaa aacagttcgc
    22561 acttcacctc gaaaagcccg tttagtaatt gaccttatca gaggtaaaag tgtcgcagat
    22621 gcaatttcaa tcttgaaatt cacaccaaac aaatctgctg gaatcattga aaaagtattg
    22681 atgtcagcag ttgcgaatgc agaaaataac tttgacttag atgttgaaaa cttggtagta
    22741 agcgaagctt ttgttaacga aggacctaca atgaaacgtt tccgtcctcg tgcaaaagga
    22801 tcagcttctc caatcaacaa acgtacaagt catatcacgg tagtcgtatc agaaaaataa
    22861 ggagggataa tcagtgggtc aaaaagtaca tccaattgga atgcgtgtag gcatcatccg
    22921 cgactgggat gctaaatggt atgctgaaaa agagtatgct gagttcttac acgaagattt
    22981 aagaatccgt aaatttattt caactaaact tgctgatgct gctgtatcaa cagttgaaat
    23041 cgaacgcgca acaggtcgcg taaacatttc aattcacaca gctaaaccag gtatggttat
    23101 tggtaaaggc ggttctgaag tcgaaagcct aagaaaagaa ttaaacaaat taactggtaa
    23161 aagaatccat atcaatatcg tggaaatcaa aaaaccagat ctagatgcaa aattagtagg
    23221 tgaaggaatc gcacgtcaat tagaaaaccg tgttgctttc cgtcgcgctc aaaaacaagc
    23281 catccaacgc tcaatgcgtg ctggcgctca agggatcaaa actcaagtat ctggtcgtct
    23341 aaacggagca gatatcgctc gttcagaagg atactcagaa ggaactgttc ctcttcatac
    23401 tttacgtgcg gacattgatt acgcatggga agaagcagat acaacatacg gaaaactagg
    23461 agttaaagtg tggatctatc gtggagaaat tcttccaact aaaaaaaaca ctgagaaagg
    23521 agggaaataa tcatgttagt acctaaacgt gtaaaacacc gtcgtgaatt ccgcggaaaa
    23581 atgcgcgggg aagctaaagg cggaaaagaa gtagcattcg gtgaatacgg tttgcaagct
    23641 gttgattcac attggatcac aaaccgccaa atcgaagctg ctcgtatcgc aatgactcgt
    23701 tacatgaaac gtggtgggaa agtatggatt aaaattttcc ctcacaaatc ttatactgcc
    23761 aaagcaattg gggtacgtat gggttctggt aaaggggcac ctgaaggatg ggttgcacca
    23821 gtaaaacgtg gtaaaatcat gtttgaaatc gcaggcgttc ctgaagaagt agctcgtgaa
    23881 gcgttacgtc tagcttctca caaattacca atgaaaacta agatcgtaaa acgtgaggaa
    23941 atgggtggtg aatcgaatga aggttaaaga aatcagagaa ttaaccactg ccgaaatgct
    24001 tgatcaagaa aaacaattaa aagaagaatt gtttaatctt agattccaat tagcaacagg
    24061 tcaattagaa aacactgcac gtattaaaga agtacgtaaa tcgattgcac gcatcaaaac
    24121 tgtgttgcgt gaacaagcta agtaattatg gaaggaggcc attaagcgta tgactgaagg
    24181 aagaaatcaa cgcaaagttt atcaaggtcg cgtggtttca gacaaaatgg ataaaacaat
    24241 taccgttgct gtcgaaacaa agaaaaacca ccctatctat ggcaaacgca tgaactattc
    24301 taagaaatac aaagtgcatg atgaaaacaa cacagcaaaa gttggcgaca tcgtgagaat
    24361 tatggaaact cgtccattat cagctacaaa acgtttccgt ttgttggaag tagtcgaaga
    24421 agcagttatt atctaataac acgagatttt cctatataga ttgaatgcaa aatctgaaag
    24481 gaggatacac aacgtgatcc aagcagaaag tcgtttaaaa gtcgctgata actcaggcgc
    24541 acgtgaaatt ttgacgatca aagtccttgg tggatctggt cgtaaaactg ctaacatcgg
    24601 tgatgtgatc gttgctacgg ttaaacaagc aacgccaggt ggggttgtca aaaaaggtga
    24661 cgtcgttaaa gccgttatcg ttcgaacaaa atcaggagct cgtcgtacag acggttcata
    24721 tattaaattt gatgaaaatg ctgcagtgat tattcgtgat gataaaagcc ctcgtggaac
    24781 acgtatcttt gggccagttg cacgtgaact acgtgaaaac aacttcatga agatcgtttc
    24841 actagcacca gaagttttat aatcttgaga cacttatcaa aggaggtgcg aaacagttat
    24901 gtttgttaaa aaaggcgata aagttaaagt tatcactgga aaagacaaaa acaaagaagg
    24961 cgttgtacta gcagcgttcc cgaaaaaaga taaagttatt gtcgaaggtg taaatgtcgt
    25021 taaaaaacac caaaaacctt ctcaagctgc tcctcaaggt ggaattgttg agatggaagc
    25081 gccaattcat gtttctaacg tgatggtggt tgactctact ggtgtagctg gccgagttgg
    25141 ctacaaagaa gtagacggca aaaaagtccg tgtttctaaa aaaaccggtg aagttttaga
    25201 taaataaaag tttaaggaag gagggtctta ctgaatgaac cgcctaaaag aaaaatatct
    25261 taaagaagta actccatcat tgatggaaaa atttaactat agctctgtta tgcaaacacc
    25321 taaagttgaa aaaatcgtca tcaacatggg tgttggtgat gcggtatcaa acgctaaaaa
    25381 cttagataaa gcagttgaag aattaacttt gatcactggt caaaaaccaa tgattacaaa
    25441 agctaaaaaa tcaatcgctg gattccgttt acgtgaagga atgccaattg gtgcaaaagt
    25501 tacattgcgc ggagaaagaa tgtacgaatt cttagacaaa ttggtttctg tttcactacc
    25561 acgtgtacgt gacttccatg gtgtaagtaa aaaagccttt gacggtcgtg gaaactatac
    25621 tttaggtatt aaagaacaat tgatcttccc agaagtagat tatgatttag tagataaagt
    25681 acgtggtatg gacatcgtta ttgtaacaac agcaaactct gatgaagaat ctcgtgagtt
    25741 gttgacacaa ttaggcatgc cattccaaaa ataattaaag gaggcgaact acgtggctaa
    25801 aaaatcaatg attgctaaaa acaaacgtcc tgcaaaattt tcaacacaag cttatactcg
    25861 ttgcgaacgt tgcggacgtc cacattcagt ttatcgtaag ttccatcttt gccgtatttg
    25921 cttccgcgaa cttgcctata aaggtcaaat tcccggcgtg aagaaagcta gctggtaaaa
    25981 agtaaattcg cataagaagg aggtaaaaga tcaatggtca tgacagatcc aattgcagat
    26041 tttctaactc gtatccgtaa cgcaaacatg gttaaacatg agtctttaga agtacctgca
    26101 tcaaaaatta aacgtgacat cgctgaaatc ttgaaacgtg aaggtttcgt tcgtgatgtt
    26161 gaatacatcg aagatgacaa acaaggcgtg atccgtgttt tcctaaaatt tggtaaaaac
    26221 ggcgaacgtg ttatcacaaa cttaaaacgt atctctaaac caggtttgcg tgcttatgtc
    26281 aaagctgacg aagtaccaaa agtattgaac ggtttaggta tcgcaatcat ttcgacttct
    26341 gaaggtgttg tcactgataa agaagctcgt gaaaaaaata tcggcggcga agttatcgct
    26401 tacgtatggt aatcaagtag aatctacaag gaggtgtctt taagtgagcc gtatcggaaa
    26461 taaagtcgtt gttatccctg aaggagtaac agttactcaa gacggaaaca acgttacagt
    26521 taaaggacca aaaggtgaat tgactcgtac tttttcagca gatatcaaaa tgaatatcga
    26581 aggaaacgaa gtaactttca ctcgtccaaa cgacagtaaa gaaatgaaaa caattcatgg
    26641 aacaactcgt gcgaacttca acaacatggt tgtaggtgtc agcgaaggtt tccaaaaagc
    26701 gttagaacta atcggggttg ggtaccgtgc acaattacaa ggatcaaaac ttgtattgaa
    26761 cgttggttac tcacatccag tagaaatcac accaccagct ggagtaactg ttgaagtacc
    26821 agctaacaca caagtaattg ttaaaggtgc gaacaaagaa gaagttggcg aattagctgc
    26881 aaacattcgt ggaactcgtc ctccagaacc ttataaaggt aaaggtattc gttatgttgg
    26941 cgaatatgta cgccgtaaag aaggtaaaac tggtaaataa tcgtttagaa aaattgcata
    27001 gaagccagca gcaggaaaaa ggcatttggc tgaagtttac cttcaaaaaa atacattttt
    27061 acttgtgccg cagcttctat ttttcgaaac gtatttactg cttgaaatag tagtacaata
    27121 aaaataaaga ggtgacaatt gtgattacaa aaccagataa gaacaaaaca cgtcaaaaga
    27181 gacatcgtcg tgtacgtaac aatatctctg gtactgctga gcgcccacgc ttgaacgttt
    27241 ttcgttctaa caaaaacatc tacgctcaaa ttattgatga cgtagcgggt gtaacgctag
    27301 caagtgcctc taccttggat aaagaaattt caggtggaac aaaaacagaa gcagcacaag
    27361 ctgtcggtaa actagttgct gaacgtgcag ctgaaaaagg cattaaagta gtagtctttg
    27421 accgtggtgg atacctttac catggccgtg tgcaagcttt agctgaagct gctcgcgaaa
    27481 atggactaga attttagaag aaggaggaat accattcatg gtttatatcg atccaaaaca
    27541 tttggaatta gaagaccgcg ttgttgcaat taaccgtgta acaaaagttg ttaaaggtgg
    27601 acgtcgtcta cgttttgctg ctttagttgt tgtcggtgac aaaaacggac acgtaggatt
    27661 tggtacaggt aaagctcaag aagttcctga agcaatccgt aaagcaattg aagatgcgaa
    27721 gaaaaactta gttgaagtac ctatggttgg ttcaacaatc ccacacgaag taatcggtgc
    27781 ttttagtggt ggtagaatcc taatgaaacc tgctgtagaa ggttctgggg ttgctgctgg
    27841 tggaccagtt cgtgccgtat tagaattagc tggggtagca gatatcactt ctaaatcttt
    27901 aggctcaaat acaccaatca acgttgttcg cgcaacagtt gaaggtttga aacaattgaa
    27961 acgtgcagaa gaagttgcag aacttcgcgg caaatcagtt gaagaattaa tcggataagg
    28021 aggacaacaa taatggctga attaaaagtt actttaaaac gcagtattat cggacgtcct
    28081 caaaaccaac gcgatacagt taaagcgtta ggtcttacaa agattaacag ttcagtggtt
    28141 aagccttcta atgaagcaat taaaggcatg atcaatactg tttctcacct agtggacgtt
    28201 gaagaaattt aatttagaaa aatcataact agaacattaa ggaggtgcca aacctatgaa
    28261 acttcatgaa ttaaaacctg ctgaaggttc acgccaagta cgtaaccgtg ttggtcgtgg
    28321 tacttcatct ggtaacggaa aaactgccgg acgcggacaa aaaggacaaa aagctcgttc
    28381 aggcggcggt gtgcgtttag gatttgaagg gggacaaaca ccattgttcc gtcgtttacc
    28441 aaaacgagga ttcacaaacg tgaaccgtaa agactatgca gtcattaatc ttgatgtatt
    28501 gaaccgcttt gaagacggag ctgaagtaac tcctgttgct ttagttgaag ctggaatcgt
    28561 gaaaaacgaa aaagctggaa tcaaagtatt agctaatggg gaactaaaca agaaattaac
    28621 tgtgaaagca gcaaaattct ctaaagcagc tcaagaagca attgaagcgg ctggtggatc
    28681 aatcgaggtg atctaatgtt tagactatta aaagatgctt ttaaagtaaa agacattaga
    28741 tcgaaaatcc tatttactgt atttgtgttg tttgtatttc gcctaggtgc acatattact
    28801 gtaccaggcg taaatgctgc ttcattgcaa aacctttctt ctttgccatt tttcaacatg
    28861 ttgaacttgg taagtggtag tgcgatgcaa aacttttcca ttttctcaat gggggtttca
    28921 ccatacatta ctgcttctat cgtcattcag cttcttcaaa tggacatcgt gcctcgattt
    28981 gttgaatggt cgaaacaagg ggaagttggt cgtaaaaaac tgaatcaagc gactcgatat
    29041 ctgacattag tattggcatt tatccaatct gtcggtatca ctgctggatt caatcaatac
    29101 gctcaattag gtttcgtgaa taatcctaac gtagcaactt acgtaacgat cggtttgatt
    29161 ctaacagccg gtacgatgtt tgttacttgg ctaggagaac agatcacaga aaaaggtatc
    29221 ggaaatggcg tatcaatgat catttttgca ggtatcattt ctagattgcc tgaaagtatc
    29281 agagaaattt atgaagatta ctttataaat attgatcctt cagacatatg gaaatctgct
    29341 atttttgtgg ccattttact agtagcggtc ttgttgatcg tgacattcgt aacatttttc
    29401 caacaagctg aacgcaagat cccgatccaa tatacgaaac gcgtagctgg cgcgcctaca
    29461 agcagctatc tgccgttgaa agtaaatgca gctggagtta ttccagttat ctttgctagt
    29521 tcgtttattg ctacgccaaa tgctgtttta caagcattga gcggtagatt cggttctgaa
    29581 aactggtaca aagtgatgat ggagatcttt aactataata cagcacctgg tgctacgatt
    29641 tatacgattc tgatcgttgc cttcactttc ttctacgcct ttgttcaagt aaaccctgag
    29701 aaattagcgg aaaacttgca aaagcaagga agttatatac caagcgtacg ccctggtaag
    29761 gggactgaag agtatgtctc aagattactg atgcgtttaa gtacagttgg tgcagtcttc
    29821 cttggtttag tggcattgct tccaatcatt gctcaaatgg tttgggatct tccacaatca
    29881 atcggattag gcggtacaag cttactgatc gttatcggtg tagctttaga aactgcgaaa
    29941 cagttagaag gcttaatgct gaaacgtaaa tatgttggat tcattgagta agaaagcgtg
    30001 ttgaggtttc ccctcaacac acgttctcac acatttatta ggagggaaaa caatgaacct
    30061 cattttaatg gggctgccag gcgcaggtaa aggaacacaa gctgaaaaaa tcattgatgc
    30121 ttatggtatt ccgcacatct caacaggaga tatgttccgc gcagcgatgc aaaacgaaac
    30181 agctcttggc ctggaagcaa agtcttatat ggataaaggt gcattagttc ctgatgaagt
    30241 aacaaatgga atcgtgaaag agcgactagc tgaaccagat acagagaaag gcttcttatt
    30301 agatggcttt ccacgtacgc tagatcaagc aaaagctctg gacgcaatgc taaaagattt
    30361 gaataaaaaa attgatgctg tcatcgatat ccatgttggt gaagaaatct tggtcgaacg
    30421 tctagccggc cgatttatct gcagcaattg tggagcaact taccataaaa ttttcaaccc
    30481 tacaaaagtt gaagacacat gtgatcgttg cggtgggcat gaattctatc aaagagaaga
    30541 tgataaacct gagacggtta aaaatcgtct ggctatcaac atcaaaaata gtgaaccaat
    30601 cttggcttat tataaagaac aaggtttgct aaacactatt gatggtgctc gtgaaatcga
    30661 tgctgtattt gcagatgtac aaaaaatcat tgaaaaatag cacgattgcc cgtagccgac
    30721 ttgcattatg cagttactct tgtcaagttc aataaattat gatagaatat gtcagtccga
    30781 cttctagaga atcctctaga tgatccattt tgagaatttt gaggtgggaa ctgcagtttc
    30841 cgccatttta tcgtgtgaaa atgcgaaaca agtacaagga ggtactgtgc gtggcaaaag
    30901 aagatatgat tgaagtcgaa ggtacagtcg tcgaaacttt gccgaatgca atgtttaaag
    30961 ttgaactaga aaacggacac caagttcttg ctactgtttc aggtaaaatt cgtatgcatt
    31021 acatccgtat cttacctggt gacaaagtaa cggttgaatt gtctccatac gatcttaacc
    31081 gtggccgtat tacttatcgc tttaaataat tgtactccgt aaaactacag gaggtattat
    31141 catgaaagta agaccatcag taaaaccaat gtgcgaacat tgtaaagtaa ttcgtcgtaa
    31201 aggacgcgtt atggtgattt gcccagcaaa tccaaaacat aaacaacgtc aaggataatt
    31261 ggaggtgcta taaatatggc tcgtattgca ggagtagata ttcctcgtga taaacgtgta
    31321 gtagtttcac ttacttatat ctatggtatt ggtaacacta ctgctaaaaa gatcttagct
    31381 gatgctggtg tatctgaaga tgttcgcgtt cgtgaattaa cgaatgaaca aacagatgct
    31441 atccgtgcag aagtagataa attaaaagtt gaaggggatc ttcgtcgtga agtgaacttg
    31501 aacatcaaac gtttgatgga aatcggttct taccgtggaa tccgtcatcg ccgtggattg
    31561 cctgttcgtg gacaaaacac gaaaaacaat gcacgtactc gtaaaggccc agctcgcgct
    31621 atcgctggca agaaaaaata attcttaagt gaaggaggtt aaaacttcat ggcagcaaaa
    31681 aaagtgaatc gtaaacgccg tgtgaagaag aatatagaag caggtattgc acatatccat
    31741 tctacattca ataacactat catcatgatc actgatactc acggaaatgc attagcgtgg
    31801 tcatcagctg gttcattagg tttcaaagga agcaaaaaat caactccttt tgccgctcaa
    31861 atggcagcag aaactgctac aaaagcagca atggaacacg gattaaagac tgttgaagta
    31921 acagttaaag gacctggttc tggacgtgaa gcagcaattc gttcattaca agctacaggt
    31981 ttagaagtga ctgcaattcg tgacgtgact ccagttcctc ataatggtgc ccgccctcca
    32041 aaacgccgtc gtgtttaatg agtgccgctc attttgagtt ttatcagtaa cgtcattgaa
    32101 caagacactt ttcgttttga aaggggtaaa gataagaatg attgagttcg aaaaaccaag
    32161 aatcgcaaaa attgatgaag aaaaagatta tggcaagttc atcgttgaac ctcttgaaag
    32221 agggtatggg actactttag ggaattccct acgtcgtatt ttattatctt ctttgccagg
    32281 tgctgccatc actaatattc aaatcgatgg tgtactgcat gaattctcta ccatcaaagg
    32341 tgttagagaa gacgtcactc aaatcatctt gaacattaaa ggcttagcgc tgaaaatgta
    32401 tggccaagaa gaaaaaaccc ttgaaatcga tattaccgga cctgctacag taactgctgg
    32461 cgatattatc gttgatagtg atgtagaaat tcttaacaaa gatatgtaca tctgtagtgt
    32521 ctctgaagga gctacgttcc atgctcgctt aacagtgaaa cctggacgtg gttatgttca
    32581 agcagatgaa aataaaaaag aagatatgcc aattggcgtt cttccagtcg attcaatcta
    32641 cacacctgtt cgtcgtgtga actatcaagt tgagaacaca cgtgttggac accgtgaaga
    32701 ttttgataaa ttgacgatgg agatttggac agatggttca attgaaccgt tggaagcaat
    32761 gagtttgtct gcgaaaatca tgactgaaca tttggatatt tttgtgaact taactgatga
    32821 agcaaaaaat gctgaaatca tggtcgaaaa agaagaaaca caaaaagaaa aaatgttgga
    32881 aatgacgatc gaagagttag acttgtctgt tcgctcttac aattgtttga aacgtgcagg
    32941 aatcaatact gtacaagaac ttacaaataa atctgagcct gaaatgatca aagtccgtaa
    33001 cttaggacgt aaatcattag aagaagttaa agcaaaattg cacgacttag gtttaggttt
    33061 acgtaaagaa gattaaaacc ttttacgaag gagggaaacc aacgtgagtt atcgtaaact
    33121 aggacgcaca tctagccaac gtaaagcgat gttgcgtgat ttaacaactg atttattaat
    33181 caacgaacgt attgtgacta ctgaagctcg tgcgaaagaa gttcgctcaa ctgcagaaaa
    33241 aatgatcact ttaggtaaac gcggagattt gcatgcacgt cgtcaagctg cggctttcgt
    33301 acgtaatgaa gttgccagcg tgcgtgaaga aaatgaggac atcgtcattg aatcagcttt
    33361 acaaaaactg ttcaacgata ttgcacctcg ttacgcagaa cgtcaaggcg gatacacacg
    33421 cattttgaag acagaaccaa gacgcggtga tggtgcacca atggttgtct tagaacttgt
    33481 ctaaaatcac actaataaca tcactttata gatgattctt ttagagtgtt atgatgttgg
    33541 aagatatttt tccgagtcta gctcagcgct gctcctcaag cgaatatcgc ttggggagta
    33601 agcactcatc ataaagtgtt tttttatacc ttcaattacc tgtcatgaca gatatgtttg
    33661 aaggcataaa aaaacatttt aagcgagaaa taattgaaaa ttttgtcttt ttttgatagt
    33721 gttagtagga actttaggat gaagaaggag taatcatgga gccaattatt gaattagaaa
    33781 agataaatta taaataccag ccagatgatc ttcgccccgc attgaaagat gtctccttta
    33841 caattgataa aggtgagtgg atcgctatta ttggacacaa tggttcagga aaatcgacac
    33901 ttgccaaaac gatcaatgga ttgcttctgc cagaatcagg gatcgtgaaa gtgggaaatc
    33961 aaatattgga tgaagaaaat atttggacga tccgacagat ggttggaatg gtttttcaaa
    34021 atccagacaa tcagtttgtt ggttcgacag tagaagatga cgttgctttt ggtcttgaaa
    34081 atcaagggat tcccagagaa gaaatgctag tgcgtgtgaa agatgcactt gagaaagtta
    34141 gaatggctga gtttgcttca cgcgagcctg ctcgtttatc aggagggcaa aaacaacgtg
    34201 ttgccattgc cggcgtggtt gccttacgac ctgatatcat tattttagat gaagcaacca
    34261 gtatgctaga tccagaaggt cgtgaagaag tgatctctac gatcaagaag atcaaagaag
    34321 aaagtcaatt gaccgttata tctatcactc atgatattga tgaagcagct aatgccaatc
    34381 ggatactagt gatgagacaa ggcgaacttg tccgtgaagg tacaccaaaa gaaatttttt
    34441 ctgctggtcc tgaactgatc gacttaggtc tagatctacc atttcctgaa aaactgaaga
    34501 gcgcactgaa agaacgtggg gttgatgtgc ccagtgaata tatgacagaa gaaaggatgg
    34561 tggattggtt atggacatcc gttttgaaca agtagacttc acttatcagc cgaatacacc
    34621 atttgaacaa agagccttat ttgacatcaa tatgacgatc aaagaaaaca gttatactgc
    34681 attagtaggg cataccggaa gcgggaaatc aaccttactt cagcatttga atgcgcttgt
    34741 taagccaaca agcggtacag tccatattgg agaacgagat attcagccgg atacggataa
    34801 taagaattta aagcccatcc gaaaaaaagt tggcatcgtc tttcagtttc cagaagcaca
    34861 gctattcgaa gaaacggtag caaaagacat tgctttcggt cctaaaaact ttggtgtcag
    34921 cgaagaagaa gcactagtcc tagcaaaaga aacattagaa caagttgggc tggatgaaag
    34981 ctatttggaa cgttcgccat ttgaactttc aggagggcaa atgcgtagag tagcaatcgc
    35041 tggtgtgctt gccatgaggc cggaagtact tgtattggat gaaccaacgg caggacttga
    35101 tccccagggt agaaaagaaa tgatggagat gttttggcgt cttcataagg aacagcagat
    35161 cactattgta ttagttacgc atttaatgga tgatgtagcc aattttgcag attatgtcta
    35221 tgtgttggaa aagggacgaa tcgttaatag cggggagccg caagaggtct ttcaaaacat
    35281 tgaatggcta aaagaaaaac aactaggcgt acctactgcc accgagtttg cagaagaatt
    35341 aatggctaaa gggatgaact ttgctacctt gcctttgaca gctgaagaat tagcagacgc
    35401 tatcgtccat tatgctggag gaaatgccca tgatgaataa attgatattt ggtcgttata
    35461 tgcctggaga ttccttgatc catcgattgg atccccgagc taaattatta gccagttttt
    35521 attttatcgg aatcattttt ttagcaaata attgggaaac gtaccttttg ttggcatttt
    35581 tcacattact ttctgtcttc ttgtcaaaag tagatttgaa attttttatc cgaggcattc
    35641 gcccattgat ctggctaatc ctcttcactg tagctttgca ggtcttgttt acaagtggcg
    35701 gagaaactta ttggagttgg ggtgttttcc atatcactga atttggtgtg atgaacggcc
    35761 tctttatttt ctgtcgattc gtgttgatca tttttatgtc gactctactc acgcttacta
    35821 caccgccttt ggaattatcg gatgcgattg agtacttgct tcgtccattg aaagccgtac
    35881 ggttcccagt ccacgaaatt tcattgatgc tgtcgattgc tcttcgtttt gtgcctacat
    35941 tgatggacga aacagaaaag atcatgaacg ctcagcgcgc tcgcggtgtt gattttggtg
    36001 aaggaaacct agttcagaaa atgaaagcag tcattccttt gctgattcct ttatttgtca
    36061 gcagcttcaa ccgtgcggaa gatcttgcga ctgctatgga agcaagaggc tacaaaggtg
    36121 gggaaggaag aacgaaatat cgtgtattgc attggcataa tagagacacg atggttatca
    36181 ttgcttttgt attattaact gtgctactga tctttttgag aggataaacg ttcagtgggc
    36241 cgctatctat tcttttcttg ctgatctgtt tgcaagaaag gatgcgagca gtgtatgaca
    36301 agaaatatta gggtctgtgg cagact
//
LOCUS       DAZKLD010000011        34287 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.011, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000011 DAZKLD010000000
VERSION     DAZKLD010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34287
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.011"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            159..1385
                     /locus_tag="ACG8KR_000484"
     CDS             159..1385
                     /locus_tag="ACG8KR_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFQ0199886.1"
                     /translation="MPTKLSNGKYKTNLRYPKRFKEITGIASEKYQKTFPNRQLAIKA
                     ENDMKKKIEKVLREENANSLELKGKITFKKFYESKWLPRYELGQTIRSNRPPSDITIS
                     NTKDIFRLHILPMFGEYAMNYLNINTEIISDELTKKSKEYANIKIIKGYVRSMFDIAE
                     ILNYIEFNRTTKIIQSITAPKKNALEEKRIQEGKQALSSKELTNWIEAVNDDFNNHLL
                     TFHDYTLFMITLYLGDRKSETYALQWKYIDFEKQTVRLKHTLDKYQRKKFTKGRKDTV
                     IQVPEVVMTLLSEWKSVQADQLLKLKIKQTLDQYLFTYTKPSGEVNCPVHADYLNYRI
                     NSIKRRHPDLVHLSPHKLRHTYATIARQGGADMNQISNALTHSDISTTKIYVNTPDIV
                     DKAVFEAFQRGLNKCD"
     gene            complement(1473..1865)
                     /gene="rpsI"
                     /locus_tag="ACG8KR_000485"
     CDS             complement(1473..1865)
                     /gene="rpsI"
                     /locus_tag="ACG8KR_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HFQ0199887.1"
                     /translation="MAQVQYIGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     REVINQPFAVTETKGAYDVFVNVNGGGYAGQSGAIRHGIARALLEVDPDFRLALKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(1881..2309)
                     /gene="rplM"
                     /locus_tag="ACG8KR_000486"
     CDS             complement(1881..2309)
                     /gene="rplM"
                     /locus_tag="ACG8KR_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003083327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HFQ0199888.1"
                     /translation="MAKPGEVERKWYVVDATDVPLGRLSTVVASVLRGKNKPTFTPHL
                     DTGDFVIVINADKVKLTGKKATDKIYYRHSNYPGGLKSITAGELRAKNSRRLIETSVK
                     GMLPKNTLGRKQFTKLNVYGGAEHPHAAQQPEVLDITNLI"
     gene            complement(2843..3310)
                     /locus_tag="ACG8KR_000487"
     CDS             complement(2843..3310)
                     /locus_tag="ACG8KR_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HFQ0199889.1"
                     /translation="MKFQQTKEVLNQLVADLSQMSVVVHQTHWYMRGPGFLTLHPMMD
                     EFMDDLNAQLDEVSERLITLDGSPYSTLREFADNTKIPDEIGRWDRTIPERLETLVAG
                     YRYLADLYQKGIEVSGEEGDDPTQDIFIEFKTATEKRIWMIQAHLGKAPEIDA"
     gene            complement(3430..4968)
                     /locus_tag="ACG8KR_000488"
     CDS             complement(3430..4968)
                     /locus_tag="ACG8KR_000488"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046316 - gluconokinase activity [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0046177 - D-gluconate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HFQ0199890.1"
                     /translation="MNVSIGLDIGTTQTKAVAFTDEAQEAASAYFRYPLIQETQGMAE
                     QDQELIFQGVCTVIKEVIKQLPSDTIIQFVSFSSAMHGLILLDEEKTPLSRMITWADN
                     RAADEAEKLKYTVLGKEFYRQTGTPIHPMTPLNKIKWLQNSQPELFKKTAYFVGIKDY
                     IFYRLFGELISDYGTASGTGYFDIHEFQWAEEVLDYLNIRIEQLPQVFPSTYQVTGLP
                     SKTAAELGLPNDISFVLGGADGPLSNLGLGAFGDTIAALTVGTSGALRFITTQPQFHP
                     EMETFCYVLDQKHWVVGGATSNGAGIFDWAGHTFLQEVVQSAKEKGENPYDALLSAIA
                     FVPAGANGLIFHPYLLGERAPLWDADAFGSFIGLQRQHTEKEMMKAILEGICLNLYRI
                     LKGVCQENQLTEIRATGGFAQSPIFRQMMADVLGYPLVFPTVTEASALGAVILGWRSI
                     GKIQSISEAQELIELKEQVGVQETDHKKYQKIYPLFVSTQKHLAQTYQQFAQLREELA
                     DSEK"
     gene            5048..5734
                     /locus_tag="ACG8KR_000489"
     CDS             5048..5734
                     /locus_tag="ACG8KR_000489"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HFQ0199891.1"
                     /translation="MLLYFIIGCCIGSFLCLTAQRIPLGHSIIYPRSHCVKCCRSLSW
                     YELIPILSIIVQRFRCRYCRCRLPFYYLLAEALCGGLFAWFFTFSSARNFSTFIWMLS
                     ALLFSLMDLFYFMVHSHTLFCSWAILWIFWLQAGVFQWQSVLLTFIVGGVCLRYGQSY
                     LGAGDTLLMLSWSGGLSLEELLQILFLASLFGISVFSVYFICHKKKLEKLPFIPFLSS
                     ALLIVLHLRY"
     gene            complement(5935..6462)
                     /gene="msrA"
                     /locus_tag="ACG8KR_000490"
     CDS             complement(5935..6462)
                     /gene="msrA"
                     /locus_tag="ACG8KR_000490"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFQ0199892.1"
                     /translation="MTKKAVFAGGCFWCMVKPFEEQPGIISVTSGYTGGHVPNPTYEQ
                     VTTGTTGHTEAVEIEYDPEVISYDQLVEIYWQQTDPTDALGQFADRGDSYRPVIYYND
                     EEERNIAERSKQALQDSGRFDKPIVTKIEPRSEFYPAEDYHQDYYKKNKLHYNLYREG
                     SGRAGFLRRHWGVKK"
     gene            complement(6546..7223)
                     /gene="pgmB"
                     /locus_tag="ACG8KR_000491"
     CDS             complement(6546..7223)
                     /gene="pgmB"
                     /locus_tag="ACG8KR_000491"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HFQ0199893.1"
                     /translation="MKIKAALFDLDGVLVDTARYHYEAWLVLANQLSIPFTEKENEQL
                     KGISRTESLERLLSFGKMEQKFSEKEKSAFAEQKNNLYLQAIQKMDETSVLPGAIAVL
                     EYLKKTNIKIGLGSASKNARLILEKTNLTSYFDVLIDGTQVSKAKPDPEVFLKGAQQL
                     NVPPNACLVIEDSEAGCQAALAGNMHVLGIGENINLPSAEYVIPDLTVFDQVRSFWHL
                     SEAVSRI"
     gene            complement(7220..8350)
                     /locus_tag="ACG8KR_000492"
     CDS             complement(7220..8350)
                     /locus_tag="ACG8KR_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HFQ0199894.1"
                     /translation="MRILIAPDSFKESLTALEAAQAIQSGFQEVFVQAEYDLLPIGDG
                     GEGTVSSLADSLRLKRQTAVVTNAFGKNKEIQYASDGQTAIFEMAEIVGLKDVPLNQQ
                     QPLRLSTRGVGELIRQLLQQGVRHIIIGVGGSSTNDGGIGMAEGLGYAFYDQDNQKVE
                     ALGENLGKIATVDKTNVPIELIKAKITVITDVTNLLCGKEGASFVFGPQKGLKENELA
                     VADQAMHDFYMRFAPQVIEASGSSAGGGMGAGLLAFASGKIVSGIDYVLDLLEFDKRV
                     QQADLVIVGEGRLDGQSLKGKAPVGIARRVPEGVPVIAICGSVGKGSEKAADVGISAV
                     FPILPAAVPLQEALQQATDNLHRTAKNIASLLQVVHFQGGNR"
     gene            complement(8350..10533)
                     /locus_tag="ACG8KR_000493"
     CDS             complement(8350..10533)
                     /locus_tag="ACG8KR_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 65 protein"
                     /protein_id="HFQ0199895.1"
                     /translation="MKVEEKQFDLEYINKYASQMTIGNGFFGIRGSQEEDYRNQVRGM
                     FAAGVYNRPLGAESAELVNLPDVIRWEIKIDGEHFSLETMKVLAYERALDLEMGELKR
                     YALIETAQGKKVEVITCRVADQNNLHQAALKVVIKPLTEDCQVDVKTGIDGQQTNFGT
                     QQLVEKELRVFGDDLISASYQTTESKIDIGIAAKFNKAGVFQAKNRRITASFSEKIRK
                     NTSFTLEKIIVLSTSLQQTDPFKKSIAQAQKSVSYTEILEQSMSYWTDFWHQHRITIQ
                     GDSSFDQLAMDFAAYHLQIMTPRNDPSVSVGAKGLTGEGYKGHVFWDTEIFILPFFLY
                     QFPEVAKNLLLYRYDRLEGARQKARDYHYQGALFPWESAHTGQEETPKFAALNIKTGT
                     RQEVASAAAEHHISADLAYAVQEYVQATEDQSFLETKGKTLIHETAAFWLSRAVKVDD
                     HLEIHRVIGPDEYTEYIDNNAYTNYLAHYNVALAVKYQAGDPLFLKECQNFLDKLYLP
                     VPNKEQLIPQDDTYLSKPVIDLTTYKERPGSQSILLDYARSEVNELQVSKQADLVMLL
                     YLLPEYFSQPIVSKNLHYYENKTIHDSSLSKAIHAIEACRIQEMEWAYHLFQEACLID
                     LGPDPHSSDDGIHAASLGALWLAVIFGFSGITVTNGTLSISPRLPSAWEKISFPFIWK
                     NHRLLFVLTKKQIVIKKETKETLPIFVNGDQYQLEKELILERRGK"
     gene            complement(10530..11555)
                     /locus_tag="ACG8KR_000494"
     CDS             complement(10530..11555)
                     /locus_tag="ACG8KR_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFQ0199896.1"
                     /translation="MKKVAIIGAGQVAEKVHAAYYQKRSDVLELAAVVDPVSQRAEDF
                     CRRTGFSHAYTSVTQMLNEISPDIVSVCTPNRFHFEHVLAALEANASVMCEKPPAMTA
                     DQARKMAETADKNGQILAYGFQHRFSDEATMIKNHLDVLGDIYYVEANALRRSGVPGW
                     GTFIDKELQGGGPLIDYGIHMLDTAMYITGFPKVKSVRAYSFQKIGTKKNSGKFGKWD
                     PSKFTVEDAIFGTLELENGKIIQLNTSFALNIREDKLNVAFCGDQAGANLYPGELFED
                     VDGKLVSLQKTTPVNDPHELSMAHFVNRALGDQTQMIADGWQGYVIQNLVENLYRSAE
                     TGEVITL"
     gene            complement(11600..12427)
                     /locus_tag="ACG8KR_000495"
     CDS             complement(11600..12427)
                     /locus_tag="ACG8KR_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFQ0199897.1"
                     /translation="MKFGTQDQAFFPRDILEKFRFIKDCGFEEYEIDGKLLIENIDTV
                     KQAIKETGIPVVTACNGYDGWIGDFIEERRLNGVRQIAEILRALSEVGGKGIVIPAAW
                     GMFTYRLPPMVSPRSKEGDYKAVSESLRYLDKVAEETGTVIYLEPLNRYQDHMINLLA
                     DARHYIDSNQLKHVQIIADFYHMNIEEDDISKALTTQKEYVGHIHLADNHRYQPGSGA
                     IDFTRHFQTLLQNEYEGYMVYECRLRGENEVKIYQESLQYLKNCLETARHLNDLEKE"
     gene            complement(12424..13491)
                     /locus_tag="ACG8KR_000496"
     CDS             complement(12424..13491)
                     /locus_tag="ACG8KR_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HFQ0199898.1"
                     /translation="MKKLVALEPRIAGFKDYEDREIEKNEVRIRVIFATPKPGTEIVD
                     FRGLSPFIDEEFSEEWRLFLPREEGSQRGVVFGEFQLGNMIVGTIEETGSLVTEYQKG
                     ELVCTYGPIRETLITNGVDNYKLRKLDRLDQWKNAVCYDPAQFALGGIREAHVRAGDA
                     VAIFGLGAIGQIAIQLAKKAGATTIVGIDPIAQRRELAEKYGADYTLDPMKADVGFRL
                     KELTDKRGVDSVIETSGSAAALQASLRGIGYGGIIAYVAFAKPFPAGFNLGREAHFNN
                     AKIVFARASSEPNPDYPRWNRKRIEDTCWELLMNGYLDCEEIIDPVVPFAESAEAYMR
                     YVDQHPEESIKMGVQVRGGEQ"
     gene            complement(13517..14353)
                     /locus_tag="ACG8KR_000497"
     CDS             complement(13517..14353)
                     /locus_tag="ACG8KR_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0199899.1"
                     /translation="MKNKKSRKILFYATILLLLAVNLFPFLIMLMTSFKDSQEAIATT
                     QTIFPKEWTFEHYQDIFNAEIFPFIMYFKNSLVISLIASVTAVVIGISGAYALSKLRF
                     KARSKINASFYTVYMFSGILIVVPLFKIISGLGLYDTNTALIITMVVQTLPTAIFMLK
                     SYFDTIPTDIEEAAMIDGLSRIQIIIRIIIPLAISGIISVFVYCFMVSWNDYLFASIF
                     LSTSEKFTLPIGLNTLFSTPDYIWGRMMAASLVTALPVIIMYALSERFIKGDLVEGGV
                     KG"
     gene            complement(14350..15219)
                     /locus_tag="ACG8KR_000498"
     CDS             complement(14350..15219)
                     /locus_tag="ACG8KR_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0199900.1"
                     /translation="MNRPRKHSDLKLAMGLLAPSMLLLAVLVLIPMLSNIQISFFDMP
                     LNPNSDAVFIGLSNYLEILSDSEFYRSLGLTVAYTFLVVAGSTALGLAVAILLNRKFR
                     FRKTVRSLILLSYVTPSISLIFAWKYMFNNSYGIVNDLLVDRLQLFNEAPLWFDQPVA
                     SFFMVVLFAIWRYYPYAFISFLAILQTVDKTLYEAAEMDGANAWQKFKIVTLPAIKPV
                     LATVIVLRSIWMFYMFTDVYLLTNKVNILGVYLYQTAFAFNDLGKAAAISILLFIIIA
                     AVVLLSRKKVDLK"
     gene            complement(15289..16572)
                     /locus_tag="ACG8KR_000499"
     CDS             complement(15289..16572)
                     /locus_tag="ACG8KR_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFQ0199901.1"
                     /translation="MKKIIVTTCLLATGFLLTACGESQSSNESQQTTIQFMHSSVEQE
                     RLSVINELVADFEKENPDIKIKQVPVEESAFNTKIVTLARSGKLPEVMEVSQDFAKVM
                     DKDELIDQTAVQNVIEEAGEDNYYDGAKNLVRSEDGKSYIAAPISGWVQGIWYDKEKL
                     SEAGFSEPENWDDILKIAQHFTDKENKQYGIAMPTAEGTMSEQAFSQFALSNGANVLD
                     DEGEVTIDTEKMREALSFYQNLSQYTMPGSNDVTEIKDAFMNGTAPMAIYSTYILPSV
                     YEAGNSENIGFAIPTQKDQAVYGTVSGLTISSGLDEKQKEAAEAFTAFLSQPKNMEKW
                     VLMSPGGAQPVNKQVVELDGYKENEVIKSFGELPSEIASAFDEVQVFGLVGEKNFTKM
                     GDITSSGVIGKAVNQVTVGNEDVDQALADAQKNTK"
     gene            complement(16586..18271)
                     /locus_tag="ACG8KR_000500"
     CDS             complement(16586..18271)
                     /locus_tag="ACG8KR_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HFQ0199902.1"
                     /translation="MYTKLLQAIYRDSDLSEKTASMIEQKVSVYQQKKGQAGKLHWDE
                     EDIFLITYGDQFFEEQQPTLRTFKKFYQKYLADTFRVVHFLPFFPYTSDDGFSVVNYQ
                     AINDRLGTWEDIEEMRSETRLMFDFVCNHMSAESTWFQEYLNLNDTYKDFFIDLPLDT
                     DTSSVTRPRTSPLLTTFIDKNGKEKHVWTTFSEDQVDLNFKNPEVLLKMIDILLFYLE
                     KGASFIRLDAVGFLWKELGTSCIHLPETHKIIQLFRAIADEAAPGTVLITETNVPHKD
                     NISYFGNGENEAQMVYQFSLPPLTLQAIRSGNTSKLKEWLKTIDFASKKTTFFNFLAS
                     HDGIGLNPVRGILPEKTILELVEQLKKEGTRVNYKKNPDGTTSPYEINATYLDALSSP
                     TDTTEWRSKRFLAAHSFLVTLPGVPAIYIQSVLGSRNDYKGVEKTGENRKINRQKFLL
                     ADIEKELQTDPLRTAVYGELTQMLSIRKKEKAFHPNSLIYLLDSADSFISFIREAKEE
                     KVLVIHNFSNQSAGYLLPKGDYLDLFSGQIIHCSQERKLIFQPYEYMWLKIIK"
     gene            18546..19571
                     /locus_tag="ACG8KR_000501"
     CDS             18546..19571
                     /locus_tag="ACG8KR_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0199903.1"
                     /translation="MKPTIYDIAKKANVSKSTVSRVLNNQPNISDKAKASVLQAIKEL
                     NYEPSKIARGLSGNGFDAILALSHRTSHTTYGNPFFSDVIQSISAVSEQNNFDLILQT
                     AKTPEEELEKCAKKIQEKMIRGIIILSSTMDETLLFELDQYNIPIVVIGKVGNDYTNI
                     YSVDTDNFQDSYDLVETLIKNGHRHIGCLHAPTEVYVSNDRLNGYRQCLFDHHLDIRN
                     EWIIDGGFRLEDSMAAMNDLMKLRERPTAIFSTDALKSLSIYKVAEQSGKQIPDDLSI
                     AGFSDPLLSSLFSPQLTRIDIPTNQLGIKATELLFQLIHHQYPEEKRIILSTEITLTD
                     SIQQQEM"
     gene            complement(20166..23819)
                     /gene="rpoC"
                     /locus_tag="ACG8KR_000502"
     CDS             complement(20166..23819)
                     /gene="rpoC"
                     /locus_tag="ACG8KR_000502"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HFQ0199904.1"
                     /translation="MIDVNKFESMQIGLASPEKIRSWSYGEVKKPETINYRTLKPERE
                     GLFCERIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVTRSKVRRERMGHIELAAPVS
                     HIWYFKGIPSRMGLVLDMSPRALEEIIYFASYVVIEPGNTNLEKKQLLTEREYREKRE
                     QYGQEFQAAMGAEAIKQLLDNVDLDAEAAELKEELKSAQGQKRTRAIRRLDILEAFRA
                     SGNQPSWMVMDVIPVIPPDLRPMVQLEGGRFATSDLNDLYRRVINRNNRLKRLLDLNA
                     PGIIVQNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRFRQNLLGK
                     RVDYSGRSVIVVGPFLKMYQCGLPKEMAIELFKPFVMRELVQRELASNIKNAKRKIER
                     QEDEVWDVLEDVIKEHPVLLNRAPTLQRLGIQAFEPVLVQGRAIRLHPLVCEAYNADF
                     DGDQMAVHVPLNEEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYYLTMEEEG
                     REGEGMVFRDQNEAVIAWRNGYVHLHSRIGVNPNSLGEKPFTEWQKERTMITTVGKII
                     FNEIMPPEFPYLNEPTDFNLTVQTPDKYFVEAGTDIPAHIKEQELVLPFKKKNLGNII
                     AEVFKRFKVTETSKMLDRMKDLGYKHSTHAGITVGIADISVLNEKQEIIENAHKQVET
                     ITKQFRRGLITDDERYERVIAVWNAAKDSIQQKLMEGLEAKNPIFMMSDSGARGNISN
                     FTQLAGMRGLMAAPNGRIMELPIISNFREGLSVLEMFISTHGARKGMTDTALKTADSG
                     YLTRRLVDVAQDVIIREEDCGTDRGLEIQAIREGNEIIESLEDRLVGRYTRKEVRHPE
                     TNELIIAGNQLISEDIAKQIVDAGVETVTIRSVFTCNTKHGVCKHCYGRNLATGSDVE
                     VGEAVGTIAAQSIGEPGTQLTMRTFHTGGVAGDDITQGLPRVQEIFEARNPKGQAVIT
                     EVTGDVIDISEDPATRTKEVTIKGKTDTRTYTVPYTARMKVAEGDMIHRGAPLTEGSI
                     DPKQLLQVRDVLSVENYLLREVQRVYRMQGVEIGDKHIEVMVRQMLRKVRVMDPGDTD
                     ILPGTLLDIADFKDQNYNTLVSGGVPATSRPVLLGITKASLETNSFLSAASFQETTRV
                     LTDAAIRGKKDPLLGLKENVIIGKIIPAGTGMQRYRDMEPKEVGVASENVYSISDIEA
                     QMAAEDAMNNQTK"
     gene            complement(23898..27515)
                     /gene="rpoB"
                     /locus_tag="ACG8KR_000503"
     CDS             complement(23898..27515)
                     /gene="rpoB"
                     /locus_tag="ACG8KR_000503"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFQ0199905.1"
                     /translation="MAGHVVKYGKHRERRSFARISEVLELPNLIEIQTDSYQWFLDEG
                     LREMFEDILPIDDFNGNLSLEFVDYELKEPKYTVAEARAHDANYSAPLHVTLRLTNRE
                     TGEIKAQEVFFGDFPLMTEQGTFIINGAERVIVSQLVRSPGVYFHGKVDKNGKEGFGS
                     TVIPNRGAWLEMETDAKDISYVRIDRTRKIPLTVLVRALGFGSDDTIFEIFGDSETLR
                     NTVEKDLHKNASDSRTEEGLKDVYERLRPGEPKTADSSRNLLNARFFDPKRYDLANVG
                     RYKVNKKLDLKTRLLNLTLAETLVDPETGEIIVEKGTVLTHQVMETLAPFIDNGLNSV
                     TYYPSEDGVVTDPMTVQVIKVFSPRDPEREVNVIGNGYPEAAVKTVRPADIIASMSYF
                     LNLMEGIGNVDDIDHLGNRRIRSVGELLQNQFRIGLARMERVVRERMSIQDTETLTPQ
                     QLINIRPVVASIKEFFGSSQLSQFMDQTNPLGELTHKRRLSALGPGGLTRDRAGYEVR
                     DVHYSHYGRMCPIETPEGPNIGLINSLSSYAKVNKFGFIETPYRRVDRETGRVTDQID
                     YLTADIEDHYIVAQANSPLNEDGTFAQDVVMARAQSENLEVSIDKVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPWVGTGMEYKSAHDSGAALLCKH
                     DGVVEYVDASEIRVRRDNGALDKYDVTKFRRSNSGTSYNQRPIVHLGEKVEKGDTLAD
                     GPSMEQGEMALGQNVLVGFMTWEGYNYEDAIIMSRRLVKDDVYTSIHIEEYESEARDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVQDGDLLVGKVTPKGVTELSAEERL
                     LHAIFGEKAREVRDTSLRVPHGGGGIVHDVKIFTREAGDELSPGVNMLVRVYIVQKRK
                     IHEGDKMAGRHGNKGVVSRIMPEEDMPFLPDGTPIDIMLNPLGVPSRMNIGQVLELHL
                     GMAARQLGIHVATPVFDGASDEDVWETVREAGMASDAKTVLYDGRTGEPFDGRVSVGV
                     MYMIKLAHMVDDKLHARSIGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTL
                     QEILTYKSDDVVGRVKTYEAIVKGEPIPKPGVPESFRVLVKELQSLGLDMRVLDIEET
                     EIELRDMDDEDDDLITVDALTKFAEQQTAKELEKKAAEQVEDEKDDVIQNFETAEDNL
                     D"
     gene            28243..29211
                     /locus_tag="ACG8KR_000504"
     CDS             28243..29211
                     /locus_tag="ACG8KR_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFQ0199906.1"
                     /translation="MKKNIIIGLGAALLLALGGCAQKDNSAEASDGQKTIGVLQLVQH
                     NSLDAAYKGFKEGLAEEGYKEGDNLTIDYQNAQNNQDNLKSMSEKLVKEDPDLLLGIA
                     TPAAQSLLNETTTIPMTVTAVTDLEQAHLVTSNEKPGGNVTGTTDMVPIEKQIGLLLN
                     IVPDAKTIGIMYNNGEANSKIQADLAEKELKKAGVKVKVLTANSTNDVQQVTKSLAKD
                     VDGIYIPTDNTFASAASVVGEVAKETKTPVVAGAVDQVKDGGLATVGIDYESLGKQTG
                     IMAAKILDGKNPSELPVETANDLELYVNKDMAKALGINPDSIKEAE"
     gene            29229..30134
                     /locus_tag="ACG8KR_000505"
     CDS             29229..30134
                     /locus_tag="ACG8KR_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0199907.1"
                     /translation="MTNLLSLFLSSASQGVLWALLAIGVYVTFRILDIADLTAEGSFP
                     LGAGIAAVSITNGYHPLTACLLGFLGGAAAGLVSGLLHTKLKIPALLAGIITMTGLYS
                     VTSRVMGAPNISLLGQPTIFHWVESFGITKDNAVLIVGLLFALIVVCLLVLFLRTETG
                     LAIRATGDNLAMSEANGINTDNMKIIGYMISNGCIALSGSLLAQNNGFADLNSGIGTI
                     VIGLASIIIAEVLLRNQPLFLRLLTVVLGAVIYRFILALVFELNVEPSDSKLASAFVL
                     VICLSFPQIQQKFHLPQKKKGGSVK"
     gene            30131..30937
                     /locus_tag="ACG8KR_000506"
     CDS             30131..30937
                     /locus_tag="ACG8KR_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0199908.1"
                     /translation="MNDVLTLENLHHTFEKGTINENHVLKGINLSLDPGDFVTIIGGN
                     GAGKSTLLNSIAGTFSVDQGQILLNGKNITKKSVVERSKSISRVFQDPKLGTAVRLTV
                     EENLALAMKRGKKRGFFRGVKPQDRTFFKDQLVRLNLGLENRLSSEIGLLSGGQRQAI
                     TLLMATLQRPELILLDEHTAALDPATSKTVMELTEKLITEQNLTAFMVTHDMEDAIRY
                     GSRLIMLHQGRIVVDIQQEEKQQLTVPQLLALFKQNSGTALKDDQVLLSS"
     gene            31094..32062
                     /locus_tag="ACG8KR_000507"
     CDS             31094..32062
                     /locus_tag="ACG8KR_000507"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HFQ0199909.1"
                     /translation="MTTKDKVLQLLKKTPDFLSGEKLAQQLEVSRTSIWKAIKELEKA
                     GYRFEHGPTGYRYLPSDVLDAKEIQQELRSFIPDLKVQTMLTSESTMKDVKLAAAQGD
                     SVSKLFVADTQEQARGRFGRPFFAEPGRGIYMSLLLHPDKRFDELPQYTIIAASACVK
                     AIESVTGRSASIKWVNDIYMDGKKVCGILSEAVSDMESGRISHIIIGIGLNFSVPQNH
                     FPADLQEKAISVFPDGQASVTRNQLISKIWQELFTYLSHPEKALDFYKEKSFVLGKTV
                     SFNQKGTTYVGLAKEIGDHGELIVQTNEQKMSLSSGEISLTSIRTQ"
     gene            complement(32379..32903)
                     /locus_tag="ACG8KR_000508"
     CDS             complement(32379..32903)
                     /locus_tag="ACG8KR_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF664 domain-containing protein"
                     /protein_id="HFQ0199910.1"
                     /translation="MDYLDMLCESVDRAYDRFERLFEGVSIEEANRFPVEDRAPQIKS
                     MTWLAWHTARELDFQIADLAGTSPIWHSQDWESRFPMTIADNEQDWKHSLEQAKQIQA
                     NQIEDLLGYLRGAADFAISYLRRLEPSELDEVIDENWTPPVTRGMRLVSIIDDAAMHS
                     GQVIYARRLLGLRD"
     gene            33190..>34287
                     /gene="scm"
                     /locus_tag="ACG8KR_000509"
     CDS             33190..>34287
                     /gene="scm"
                     /locus_tag="ACG8KR_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HFQ0199911.1"
                     /translation="MKKRASLLVLVALLFNIFSYGVAVSAETYIPKDPGNIYNDLTGN
                     YGLLGIASQFHVFAKNKTTVNAHTDGNIATHELDAKNNFGTELHEGLLNQEINYIQKT
                     DSFANSSGIPTGSTRMNKFVVGSNVDVALQDGHPTVNGSRLDHLTPDDFYKDRAGHEY
                     IDFEQEFAKLNVAANDLATITPAKTYTAADFPDMNNRTIDLTDLSDTGFLLVNIDAEV
                     LTMNTPLQIINPNDQVVVFNVINSSSALNVQSPIKYNDRSNHETEDFSDANISWNFGN
                     EMTDLTISAPFQGTILAPNATIRITQNQDGTIIGTNVILDAATNRWDPNEIFISDNGT
                     DTTDTSDSTDTSTTSDSSDSSTSSDSTDTTST"
BASE COUNT         9786 a         7279 c         6420 g        10802 t
ORIGIN      
        1 aatttcccag taataagagt tggtggtgta gttcgctttg acgtagaaga tattaaagac
       61 tttatgaata catttaaaat aacttgaaat tttcttctcg ccgcatatac taaaattagt
      121 gaaatatact gccttacttt ttttaaggag tgaggatttt gcctacaaag ctttcaaatg
      181 ggaaatataa aactaatctg agatatccaa aaaggtttaa agaaataaca ggaattgcat
      241 cagaaaagta tcaaaaaaca tttccaaata ggcaattggc gattaaagcc gaaaatgata
      301 tgaagaagaa aatagaaaaa gttttgagag aggagaatgc aaactcatta gaattaaaag
      361 gaaaaataac gtttaaaaaa ttttacgaat ctaaatggct tcctagatat gaattaggtc
      421 aaacaattag atcaaacagg cctcctagtg atataaccat tagtaataca aaagatatat
      481 ttcggttgca tattttacct atgtttggtg aatacgcaat gaattatcta aatatcaata
      541 ctgagataat atcagatgaa ttaaccaaaa aatcgaaaga atatgcaaat ataaaaatca
      601 taaaaggata tgttcgttcc atgttcgata ttgcagaaat actaaattat atcgaattta
      661 atcgaactac taaaattatt caaagtatca ctgcacctaa aaaaaatgct ttagaagaaa
      721 aaagaataca agaaggtaag caagcactat catcaaaaga acttactaat tggatagaag
      781 cagtaaatga tgattttaat aatcatttac ttacttttca tgattacaca ctttttatga
      841 ttaccctgta tcttggtgat agaaaaagtg agacttacgc tttgcaatgg aaatatattg
      901 attttgaaaa acaaacggtc cgtcttaaac atactttaga taaatatcaa agaaaaaagt
      961 ttacaaaagg tcgaaaagat accgtcattc aagtaccaga agttgtaatg acattgctaa
     1021 gtgaatggaa atcggtacaa gctgatcaat tgttgaaatt aaaaattaag caaacgttag
     1081 atcagtatct ctttacttat actaaaccat ctggagaagt aaattgtcct gttcatgccg
     1141 attacctaaa ctatcgaatc aattcaatta aaagaaggca tcctgactta gttcatttat
     1201 ctccacacaa actccgtcat acttatgcaa ctatagctag acaaggtgga gcagatatga
     1261 atcaaatttc aaatgcttta acacactcag atatttctac aacaaagata tatgtgaaca
     1321 caccagatat agttgataag gctgtttttg aagcttttca gagaggattg aataagtgtg
     1381 attgatatgt gttcttaaat gtgttcttaa aaacaaaaga agcactttcc aaataattga
     1441 aaagtgccag aaacgttgat atataaagga tattaacgtt ttgagaattg tgaagcttta
     1501 cgtgctttct taagacctgg ttttttacgt tcgaccatac gtgcgtcacg agtaagtaag
     1561 ccagcacgtt taagagctaa acggaagtca ggatcaactt ctagcaatgc acgagcgata
     1621 ccatgacgga tagctcctga ttgtccagcg tatcctccgc cgtttacgtt aacaaatacg
     1681 tcatatgcgc cttttgtttc agtaacagcg aaaggttgat tgattacttc acgcaagtca
     1741 gcatgtggaa tgtattcttc aacatctttt ttatttacag taattttgcc agttcctggt
     1801 actagacgta cgcgagcaac tgcgttttta cgacggcctg tgccgatata ttgaacttgt
     1861 gccaatgaaa ttccctccta ttagattaag tttgtgatgt ctaaaacttc tggttgttgt
     1921 gctgcatgtg gatgttctgc tccaccgtat acgttcaatt ttgtaaattg tttgcggcct
     1981 aaagtgtttt taggaagcat accttttaca gaagtttcga tcaaacgacg agaattttta
     2041 gcacgcaatt cgccagcagt gattgatttc aatcctcctg ggtagttgct gtgacggtaa
     2101 taaattttgt cagtcgcttt tttacctgtt aatttcactt tgtctgcatt gatgacgatg
     2161 acgaaatcgc cagtatccaa atgaggtgtg aaagttggtt tatttttacc acgtagtaca
     2221 gatgcaacaa ctgttgaaag acgtcccaat ggaacatcag ttgcgtctac tacataccat
     2281 ttacgttcta cttcgcctgg tttggccata tatgttgtac gcacgttatt ttcctccaat
     2341 ttaagttctg atgtttgaca tacacttcga gtttccgggg ctctttgtgg ggcaaacaat
     2401 accatttaac atattacccg ctaatctagt caatgtcaac gtttttctgc ttgattgaaa
     2461 aatttatatg aaactttact tcttttcttt cttttatata aaagggtcgt ttcctgtcaa
     2521 aatgaaaaac aaaaagaggc tgtgacaaaa gtcttgtcac aaccccttat tttgaataaa
     2581 cggtgttctc ctgccaactc ctcggtcaca agtcacaata tccataaaac ataagaagct
     2641 gttttttaaa attgtttctt gtgactcgga gctgaacggc aggcttacaa cctcttggta
     2701 aacggtgttc aatcagctga ccctcggtat tagctcaaaa aacggcaaaa tatgaggaac
     2761 tattttgacg tttgtttctc taatactcag gtcaaaacgc tgatttcaca acctcttttt
     2821 cggtcaagtt caacaacagt ttttacgcgt cgatctctgg cgctttgccc aaatgagctt
     2881 ggatcatcca gatacgtttt tctgtggctg ttttgaattc gataaagata tcttgtgttg
     2941 gatcgtcgcc ttcttctcca gaaacttcga ttcctttttg gtaaagatct gctagataac
     3001 gatagcctgc aacaagtgtt tccaaacgtt ctggaatagt acgatcccag cggccaattt
     3061 catcagggat ttttgtgtta tctgcaaatt cacgcaatgt agaataaggt gaaccgtcta
     3121 atgtgatcaa gcgttcggat acttcatcta actgagcgtt caaatcatcc atgaactcat
     3181 ccatcattgg gtgcaatgta aggaaacctg gtcctctcat gtaccaatgt gtttggtgaa
     3241 caacaacaga catttggctt agatctgcaa ctaattgatt taatacttct ttggtttgtt
     3301 ggaatttcat ctttcttgcc tcctaacatt ttcttacact taaaataata gtagactttc
     3361 aatgaaaagt acaacaaaat catttacctg agaacgattt tcagttcgct tttttatccg
     3421 taaagccaat catttttcag aatcagctaa ttcttctcgc agttgagcaa attgctgata
     3481 agtctgtgct aaatgctttt gtgtcgaaac aaataatgga tagattttct gatatttttt
     3541 atgatcagtt tcttgtactc caacttgctc tttcaattct atcaattcct gtgcttcact
     3601 gatactttgg atctttccga tgcttctcca gcctaaaatc acagctccta atgcggatgc
     3661 ttctgtgaca gtagggaaaa caagcgggta acccagtaca tcagccatca tctgacgaaa
     3721 aatcggagat tgagcaaacc cgcctgtagc acggatctct gtgagctgat tttcctggca
     3781 aactcctttt aaaatccgat aaaggttgag acagatccct tctagaatcg ccttcatcat
     3841 ttctttttct gtatgttgcc gctgtaaacc gataaaactg ccaaacgcat ctgcgtccca
     3901 aagcggtgct cgctcaccaa gtaaataggg atgaaaaatc agaccattgg cacccgctgg
     3961 cacaaaagcg atagccgaaa gcagtgcatc atatggattt tctccttttt ctttagcaga
     4021 ttgaacgact tcctgtaaaa atgtatgccc tgcccaatca aagatgccag caccattgct
     4081 agtcgctcct cccactaccc aatgtttctg atccagaaca taacaaaacg tctccatttc
     4141 tggatggaac tgaggctgag tagtaataaa acgaagtgct ccgctcgttc ctacggtcaa
     4201 agcggcgatc gtatcaccaa aagcacctaa ccctagatta gaaagcggcc cgtctgcccc
     4261 tcccaaaaca aatgatatat catttggcaa acctagttct gctgctgttt ttgatggtag
     4321 acctgttact tgataggtgg aaggaaagac ttgcggcaat tgctctattc gaatgttcaa
     4381 gtagtcaagc acttcttctg cccattggaa ttcatggata tcaaaatatc cagttcctga
     4441 agctgttccg tagtcgctga tcagttcgcc aaacaatcta taaaagatat agtctttgat
     4501 accgacaaaa taagcagttt ttttgaataa ttcaggttgg ctgttttgca gccacttgat
     4561 tttgttcaat ggtgtcattg gatgaatcgg cgtcccagtc tgccgataaa attctttgcc
     4621 aagcacggta tatttcagtt tttctgcttc gtccgctgct cggttatctg cccaagtgat
     4681 cattcgagat aacggtgttt tctcttcatc caacaatatc aggccatgca tcgcagagga
     4741 gaaggagaca aattgaatga ttgtgtctga aggcaactgt ttaattactt cttttataac
     4801 ggtgcagaca ccttgaaaga tcaattcctg atcttgttct gccatccctt gtgtttcttg
     4861 gatcaaaggg tagcgaaaat aagcggatgc tgcttcttgg gcttcatctg tgaaagcaac
     4921 tgctttggtt tgtgtggttc cgatatctaa tccgattgaa acgttcattt tttcacctca
     4981 aattttatgt atgtacaaaa atttagttta agcttgcgta tcttggtttt gaaaaggggg
     5041 agatggcgtg ttactttatt ttatcattgg ttgctgtatc ggatcatttt tatgtttaac
     5101 cgcccagcgg attccgttag gtcactctat tatttatcct agatctcatt gtgtcaaatg
     5161 ctgtcgttcg ctttcttggt atgaattgat tccgattctc tcgatcatcg tccaacgatt
     5221 tcgctgccgc tattgcaggt gtcggttgcc cttctactat ttgttggcag aagccttatg
     5281 tggtggtctt tttgcctggt tctttacttt ttcttccgca agaaattttt cgacgttcat
     5341 atggatgctt tcggctttgc tattttcgtt gatggattta ttttacttca tggtccattc
     5401 tcacacgcta ttctgttctt gggcaatttt atggattttt tggttgcagg ccggcgtctt
     5461 ccaatggcaa agtgtgctgt taacgtttat cgtaggcggt gtgtgccttc gctatggtca
     5521 atcctatctc ggagctggcg atactctgtt gatgctttct tggagtggtg gcttgtctct
     5581 agaagagttg cttcagatct tgtttcttgc tagtctgttt ggaatatccg ttttttctgt
     5641 ttactttatc tgtcataaaa aaaagttgga aaagctgcct ttcattcctt ttttgagcag
     5701 tgcgttgctg attgtccttc atcttaggta ttgaaaaaag gttgtgacaa acgacttgtc
     5761 acaacctctg atatatagtg ttctcttacc aaatccttgt gacttggagc taggcgacag
     5821 gttcacaacc tctccactaa atggtgttca atcagctgac cctcagtatt agctcaaaat
     5881 gctggtttta caacctctga tatacggtgt tccataagta gctttttcgg cttcttattt
     5941 cttgacgccc caatgtcgac gaaggaaacc tgcacgtcca gaaccttctc gataaagatt
     6001 gtaatggagc ttgtttttct tgtaataatc ctgatggtaa tcttctgcag gataaaattc
     6061 gcttcttggt tcgatttttg tcacgattgg cttatcgaat cgcccgctgt cttgcaatgc
     6121 ttgttttgaa cgctctgcaa tatttcgttc ttcttcatca ttatagtaga tcactggacg
     6181 ataagaatca ccgcgatcag cgaactgtcc taatgcgtca gtcggatcgg tttgctgcca
     6241 atagatttcc accaactgat cataagaaat aacttctgga tcatattcga tctctactgc
     6301 ttctgtatgc cctgttgtgc ctgttgttac ttgttcataa gttggatttg gtacatgccc
     6361 gccagtataa ccggatgtca cagaaatgat ccctggctgc tcttcaaatg gtttgaccat
     6421 acaccaaaag catccgcctg cgaaaactgc tttttttgtc atataaaatc cccctttttt
     6481 ctcatcatac cttgatttcc aaaaggagga taacgttttg cttgtgacag aaaaaacgga
     6541 aactattata tacgtgatac ggcttctgat aaatgccaaa acgaacgtac ttgatcaaaa
     6601 acggttaaat caggaatcac atattcagca gaaggaagat ttatgttttc acctattccc
     6661 aatacatgca tgttaccagc caaagctgct tgacaaccag cttctgaatc ttcaatgacc
     6721 aagcatgcat ttggcggtac attcaattgc tgtgctcctt tgagaaaaac ttctggatca
     6781 ggttttgctt ttgatacttg cgtgccgtca ataaggacat caaaataact tgtcaaattc
     6841 gttttttcta aaatgaggcg tgcatttttg cttgctgatc ccaatccgat cttgatgttt
     6901 gtttttttca agtactcaag aacagctatt gcaccaggca aaacggatgt ttcatccatt
     6961 ttttgaatcg cttgaagata tagattgttt ttttgctcag caaaggctga tttctctttt
     7021 tctgagaatt tctgttccat ttttccaaat gacagcaatc gctctagtga ctcggttctg
     7081 gatattcctt tcagttgctc gttttctttc tcagtgaatg gaatggacag ctggttagct
     7141 aaaaccagcc acgcttcgta atgataacga gctgtgtcta caagaacgcc atctaaatcg
     7201 aatagtgctg ctttgatttt catcgattac ctccttgaaa atgaacgact tgaagtaaag
     7261 atgcgatgtt tttagctgtg cgatgaagat tatcagtggc ttgctgtaaa gcttcttgta
     7321 aaggcacagc tgcaggaagg atcggaaaaa cggccgaaat gccaacatct gctgcttttt
     7381 cgcttccttt accaacactt ccgcaaatag ctatcacagg aacgccctct ggtactcttc
     7441 tagcaatccc aactggcgct tttcctttta gactttgtcc atctagacgt ccttcgccta
     7501 cgattacgag gtctgcttgc tgtacccgtt tatcgaactc cagtaaatcc aatacgtagt
     7561 cgatgccaga gactattttc cctgaagcaa atgctaacag tcctgcaccc attccaccac
     7621 cagcactact gcccgatgcc tcgattactt gaggggcaaa tctcatataa aaatcatgca
     7681 tggcttggtc agccactgct aattcatttt ctttcaagcc tttttgtggt ccgaaaacaa
     7741 aagaagcgcc ttcttttccg cataaaagat tagtaacatc agtaatgaca gtgattttgg
     7801 ctttaatcag ctcaataggc acgttcgttt tatcaactgt agcaatttta cctagatttt
     7861 ctcccagtgc ttctactttt tgattatctt gatcgtaaaa agcataacct agaccttctg
     7921 ccatgccgat gccgccatcg ttggtactac ttccgccaac gccgatgatg atgtgtcgaa
     7981 caccttgttg caacaattgt cgtatcagtt ctcctactcc tcgcgtggat aacctaagtg
     8041 gctgttgttg gtttagtggg acatctttca atccgacgat ttctgccatt tcaaaaatcg
     8101 cagtttgacc gtctgaagcg tactggattt ctttgttttt gccaaatgca ttagtaacga
     8161 ctgctgtttg ccgcttcaac cttaaactgt ctgctaatga tgaaaccgtt ccttctcctc
     8221 catcgcctat aggtagcaga tcatattcag cctgaacaaa aacttcttga aaacctgatt
     8281 gaatggcttg tgctgcctct aaggctgtca gactttcttt gaaagaatct ggcgcaatca
     8341 gaatacgcat tattttcccc tccgttccaa gatcagttct ttttccagct ggtattggtc
     8401 tccattgaca aaaataggta atgtttcttt cgtctctttt tttattacta tttgtttttt
     8461 tgtcaaaaca aatagcaacc tgtgattttt ccaaataaaa ggaaaagaaa ttttttccca
     8521 tgctgaagga agtcttggag aaatagaaag agttccattc gttactgtaa tacctgaaaa
     8581 accaaatata actgctagcc atagtgctcc taatgaagca gcatgaattc cgtcatctga
     8641 tgaatgcgga tctggtccta aatcaatcag gcacgcttct tggaataaat gatacgccca
     8701 ttccatttct tggatacggc aagcctctat ggcgtgaatc gctttactca acgatgagtc
     8761 gtggattgtt ttgttttcat agtaatgcaa attcttagaa acgattggtt gagaaaaata
     8821 ctcaggtaat aagtataaca gcatgacaag atctgcttgc ttgctgactt ggagttcatt
     8881 tacttcgcta cgggcataat caagtaaaat agactggctg ccaggacgtt ctttgtaagt
     8941 ggtcaaatca ataactggct tactgagata cgtgtcatct tgcggaatca gctgttcttt
     9001 gttaggcact ggcaaatata acttatctaa aaaattttga cattctttca aaaaaagcgg
     9061 atcgccagct tgatatttaa cagctaaggc cacattataa tgcgccagat agttcgtata
     9121 tgcattattg tcgatatatt ctgtgtattc atctggacca atgactctgt gtatctctaa
     9181 atgatcatcg acttttacag ctctgctcag ccaaaaagct gctgtttcat gaatgagcgt
     9241 ttttcccttt gtttctaaaa acgattgatc ctctgttgct tggacatact cttgaacggc
     9301 ataagcaagg tctgcactga tatgatgttc tgcggcagct gaagcaactt cttgtcttgt
     9361 tccggttttg atattcaatg cagcaaattt gggtgtctct tcctgaccag tgtgtgcgct
     9421 ttcccatggg aaaagtgcac cttgatagtg ataatctctt gctttttgcc gtgcgccttc
     9481 taatcgatca taacggtaca gcagcaaatt ctttgctact tcaggaaact gatataaaaa
     9541 gaaaggcagg ataaaaattt ccgtatccca gaaaacatgc cctttataac cttcgccagt
     9601 tagtcccttc gcccctacac taacacttgg atcattgcgg ggagtcataa tctgtaaatg
     9661 gtaagcagcg aagtccatcg ctaactgatc gaaagaagaa tcgccttgga tcgttatccg
     9721 atgctgatgc caaaaatctg tccaataaga catcgactgc tctaaaattt ctgtataact
     9781 aacgcttttc tgggcttggg caatactttt cttaaacggg tctgtttgct gaagagaggt
     9841 agagagcacg atgatcttct ccagcgtaaa ggacgtattt tttctgatct tttctgaaaa
     9901 ggaagcggtg atccggcgat tttttgcttg gaatacacct gctttattga atttagctgc
     9961 aatcccaata tcaattttac tttctgttgt ctggtaggat gcactgatca aatcgtcacc
    10021 aaatactcgc agttcttttt caaccaactg ctgtgtacca aaattggttt gctgtccatc
    10081 gatacccgtt ttcacatcta cttggcagtc ttcggtcaac ggcttgatga ccactttcag
    10141 ggctgcctga tggagattat tttgatcagc aacacgacaa gtaatgactt ccactttttt
    10201 tccttgagca gtttcaatga gcgcatagcg tttaagctct cccatttcta aatctagtgc
    10261 ccgctcgtat gcaagaactt tcatcgtctc aagagaaaaa tgttcaccgt cgatttttat
    10321 ttcccatctg atgacatctg ggagattcac taactcggct gattctgctc ctaaaggtcg
    10381 gttatataca ccagctgcaa acattcccct tacttgattc cgatagtctt cctcctggct
    10441 tcctctgata ccaaaaaatc catttccaat ggtcatctga ctcgcatact tattgatata
    10501 ctctaaatca aactgttttt cttctacttt cataaggtaa taacttctcc tgtttctgcc
    10561 gaacgatata aattttccac cagattctgg atgacatatc cttgccagcc atctgcaatc
    10621 atctgagtct ggtcaccgag cgcccgattc acaaaatgag ccatacttag ttcatgtggg
    10681 tcattaactg gcgtggtctt ttgtaaggaa accagctttc catctacatc ttcgaatagt
    10741 tcgcctggat acagattagc acctgcctga tcgccacaaa aagccacatt cagtttgtct
    10801 tctctgatat tcaaagcaaa cgaagtattc agttggatga tcttgccgtt ttctaattcc
    10861 agtgtgccaa aaattgcatc ttctactgtg aatttgcttg gatcccattt cccgaatttc
    10921 cctgaatttt ttttcgtacc aattttttgg aagctgtacg cccgaacgct ttttacttta
    10981 ggaaatccag taatatacat cgctgtatca agcatgtgaa tgccataatc gatcaaaggc
    11041 ccgcctcctt gaagttcttt gtcgataaac gttccccagc ctggaacacc agaccgacga
    11101 agcgcattcg cttctacgta ataaatatcc ccgagaacat caagatgatt cttgatcatt
    11161 gttgcttcgt cagaaaaacg atgctggaat ccataagcca aaatctgccc gtttttatcg
    11221 gctgtctctg ccatctttct tgcttgatca gctgtcatag ctggtggttt ctcgcacatc
    11281 actgaagcat tagcttcgag ggcagccaat acatgttcga agtgaaagcg attgggtgta
    11341 cagacactca cgatatcggg agaaatctca ttcagcattt gtgtaactga tgtatacgca
    11401 tgagaaaatc ctgtacgtcg gcaaaagtct tctgctcgct ggctgactgg atcaaccaca
    11461 gcggccaatt ctaagacatc tgatcttttt tgataataag cagcatgtac tttttctgct
    11521 acttgtccag cgcctatgat tgctactttc ttcatttatt tctccctcct ttgcttattt
    11581 acttattgtt attctttctc tattctttct ctaaatcatt taaatgacgc gctgtttcca
    11641 gacagttttt caagtattgc aatgattcct gataaatttt cacttcattt tctcctcgca
    11701 aacgacattc atataccata taaccttcat actcattttg aagtagcgtt tgaaaatggc
    11761 gagtaaaatc aattgcgcca gatcctggtt ggtagcgatg attatctgct aaatggatat
    11821 gacccacata ctctttttgc gtagtcaatg cttttgaaat atcatcttct tcaatattca
    11881 tatgataaaa atcagcgatg atctgcacat gtttcaattg attagagtca atgtaatgac
    11941 gcgcatccgc taacaaattg atcatatggt cttggtatcg gttcaaaggc tccaaataaa
    12001 taaccgtacc tgtttcttcg gctactttat ctaaataacg aagtgattcg ctgactgctt
    12061 tatagtctcc ttctttgctt cggggtgaaa ccatcggcgg taatcgataa gtgaacattc
    12121 cccaagctgc tgggataacg attcctttgc ctccaacctc gcttagtgca cgcaaaatct
    12181 cagcaatttg acgtacacca ttaagacgcc gttcttcgat aaagtcaccg atccacccat
    12241 cgtagccgtt acaagctgtc actactggga tccctgtttc tttgattgcc tgtttgactg
    12301 tgtcgatatt ttctattaac aacttaccgt cgatttcgta ttcttcaaat ccacagtctt
    12361 tgataaatct aaatttttct aaaatgtctc ttgggaaaaa tgcttgatct tgcgtaccga
    12421 atttcattgt tctcctcctc ggacttgaac gcccattttg atactttctt ctgggtgctg
    12481 gtctacataa cgcatgtaag cttctgcgct ttctgcaaat ggcacaactg gatcaatgat
    12541 ttcttcacaa tccaagtaac cgttcatcaa taattcccaa catgtgtcct cgattcgttt
    12601 acggttccat cttgggtaat ctgggtttgg ttcactagat gctcgagcaa agacgatttt
    12661 tgcattattg aaatgtgctt cgcgaccaag attaaaccca gctggaaaag gtttagcaaa
    12721 ggcaacataa gcaatgatcc cgccatagcc gatccctctt aaagaagctt gtaatgcggc
    12781 agcactgcca ctcgtttcaa taactgaatc tactccacgt ttatctgtca attcctttag
    12841 acgaaaaccg acatctgctt tcataggatc tagcgtgtaa tcggcaccgt atttttctgc
    12901 taattctcta cgctgcgcta tgggatcgat cccaacgatg gtagtagcac ctgctttttt
    12961 agctaattga atcgcaattt gcccgatagc gcctaaaccg aagattgcta cagcatcacc
    13021 tgctcgaaca tgtgcttccc ggataccacc taaagcgaat tgagcaggat cgtaacatac
    13081 tgcatttttc cattgatcca accgatcaag tttacgtagt ttataattat caacaccgtt
    13141 tgtaatcagc gtttcacgaa tgggtccata agtgcagact aactctcctt tttgatactc
    13201 tgtcaccaaa ctgcccgttt cttcgatcgt accgacaatc atatttccta attgaaattc
    13261 gccaaatacc acaccgcgtt ggcttccttc ttctcttggc aagaataggc gccattcttc
    13321 tgaaaattct tcatcgataa atggacttaa tcctctaaag tcgacaattt ctgttcctgg
    13381 ttttggagta gcgaaaatta ctcggatacg tacttcgttc ttttcgatct cacggtcttc
    13441 gtaatctttg aatccggcta ttcttggttc taatgcaact aattttttca tcttttcttt
    13501 cctctttctt ttctttttat ccttttacgc cgccttcgac taaatcacct ttaataaatc
    13561 gttcagataa cgcatacata atgatgactg gtaaagcggt caccaatgaa gcggccatca
    13621 ttcgtcccca aatgtagtct ggtgtactga ataatgtgtt caaaccgatt ggtaaagtaa
    13681 atttttcgga ggtcgacagg aaaatcgaag caaataaata atcattccac gaaaccataa
    13741 agcaataaac gaagacagaa atgattccag aaatagccaa tggtataatg atccgaataa
    13801 tgatctggat ccggctaaga ccatcgatca ttgctgcttc ttcaatatca gtcggaatcg
    13861 tgtcaaaata actttttagc atgaatatcg cagtaggaag tgtttgaacg accatcgtga
    13921 taatcagtgc tgtattggta tcatacaaac ctagaccgga aatgatttta aataatggaa
    13981 caacgatcaa aataccggaa aacatataaa cggtgtaaaa gctagcattg attttcgagc
    14041 gagctttgaa gcgtagtttt gacaaagcgt atgccccgct gatcccaatc actactgcag
    14101 ttacggaggc tatcaaggag atcactaaac tatttttgaa atacatgatg aatggaaaaa
    14161 tctcagcatt gaaaatatcc tgataatgtt caaaagtcca ttctttcgga aaaatagttt
    14221 gtgtcgttgc aattgcttct tgtgaatcct tgaatgatgt catcagcatg atcaaaaatg
    14281 gaaacaaatt gacagctaat agcaacaaaa tcgtcgcgta aaataagatt ttcctgctct
    14341 ttttattttt catttcaagt ccaccttctt tcgtgagagc aagacgaccg ctgcaataat
    14401 gatgaacaat agaatcgata tagctgctgc ttttccaaga tcattgaacg caaaggctgt
    14461 ctgatacaaa taaacgccta agatattgac tttattcgtc agtagataga catctgtaaa
    14521 catatagaac atccagatag atcgtaaaac aatcacagtt gctagtactg gtttgattgc
    14581 cggcagtgtc acgatcttga acttctgcca agcatttgca ccgtccattt cagctgcttc
    14641 ataaagcgtt ttgtcgactg tttgcaaaat agccaaaaat gaaataaacg catacggata
    14701 gtatctccag atagcaaaca aaacgaccat aaagaagcta gcaactggct ggtcgaacca
    14761 cagcggtgct tcgttgaaaa gctgcaacct atctactaac agatcattga cgattccata
    14821 actattattg aacatatatt tccaagcaaa aatcaaagaa atagatggcg taacataact
    14881 taataaaatc agtgaacgca ccgttttcct aaatctgaac ttgcggttaa gcaaaatagc
    14941 tacggctaat cctaaagctg tactgcctgc tacaactaag aatgtgtaag caactgttaa
    15001 acctaaagaa cgataaaatt cactatcact taaaatttca agataattgc ttaatccgat
    15061 aaaaacggcg tctgagttag gattcagcgg catatcgaaa aaactgatct gaatattgga
    15121 aagcattgga atcagcacta aaacagctaa taaaagcatg cttggtgcta aaagtcccat
    15181 ggctaatttt aagtcagaat gtttccttgg acgattcatt gaaacctccc cctgataatc
    15241 tgccaatagc tatctacttt tttcgaacac cattggcaga acttcttgtt atttcgtatt
    15301 tttttgtgcg tcagctaaag cttgatcgac atcttcgttt ccgacagtta cttggttcac
    15361 tgcttttccg atcacacctg agcttgtgat atctcccatt ttcgtgaaat tcttttcccc
    15421 gactagtcca aagacttgta cttcatcaaa agcactagca atctcagatg gcagctcgcc
    15481 aaaagacttg atgacttcat tttctttgta accatccagt tcgactactt gtttattcac
    15541 tggttgagca ccaccaggag acatcaagac ccacttttcc atgtttttcg gttgagataa
    15601 gaaagctgtg aatgcttcag cagcctcttt ttgtttctcg tctagaccag aagaaatcgt
    15661 caatccagat actgtgccat agacagcttg atctttttgt gttgggatag caaaaccgat
    15721 attttctgag ttccccgctt cataaacaga aggcaaaatg taagttgaat agattgccat
    15781 tggtgctgtt ccattcatga aagcatcttt gatttctgtc acatcattag agcctggcat
    15841 cgtgtattgg gataagtttt gataaaacga caatgcttct ctcatctttt ccgtatcgat
    15901 cgttacctcg ccttcatcat ccaaaacatt tgctccattt gacaaagcaa attgagagaa
    15961 cgcttgctca ctcattgttc cttcagcagt aggcatagca attccatatt gtttattctc
    16021 tttatctgtg aaatgttgag caatttttaa aatatcatcc caattttctg gttctgaaaa
    16081 accagcttct gataattttt ctttgtcata ccaaatacct tgaacccagc cgctaattgg
    16141 tgcagcaata tagctttttc catcttctga acgaacaagg tttttggctc cgtcataata
    16201 attatcttct ccagcttcct cgataacgtt ttggactgcc gtctgatcaa tcagttcatc
    16261 tttatccatc actttagcaa aatcctgact gacttccatt acttctggta atttaccgga
    16321 acgagctaac gtaacgattt ttgtattgaa cgctgattct tctaccggca cttgcttgat
    16381 tttgatgtct ggattttctt tttcaaaatc agcaaccagt tcattgatca cacttaatcg
    16441 ttcctgttcg acagaagagt gcatgaattg aatcgttgtc tgttgtgatt cgttagagct
    16501 ttgactttca ccacaagcag taagaagaaa tccagtagct agcagacagg tagtaacaat
    16561 aatttttttc atttttttcc acctcttatt ttattatttt cagccacata tattcatacg
    16621 gctggaatat caactttcgt tcttgactgc aatggataat ttgtccagaa aacaggtcta
    16681 ggtaatctcc ttttggtaaa agataacctg ctgattgatt ggaaaaatta tggataacta
    16741 agactttctc ttccttcgct tcgcgaataa aactgataaa agaatctgct gagtccagta
    16801 agtaaatcaa actattcgga tggaaggctt tttctttttt tcgaatagat agcatctggg
    16861 tcaattcacc atatactgct gttcttaatg gatctgtctg caactctttc tcaatatctg
    16921 ctaacaaaaa tttttgccga ttgatttttc gattctctcc tgttttttct acgcctttgt
    16981 aatcatttcg acttcccagc acactttgaa tataaattgc cggcacgcct ggtaacgtta
    17041 ccaaaaatga atgagcagct aaaaagcgtt tgcttctcca ttccgtcgta tctgttggag
    17101 aacttaacgc atctagatac gtagcgttta tctcatatgg actggtcgtt ccatctgggt
    17161 ttttcttgta gtttactcgg gtaccctctt tcttcagctg ttccactaat tcaaggatcg
    17221 ttttttccgg cagaatacct cgtaccggat tcaatccgat accgtcatgg gaggctaaaa
    17281 aattaaaaaa agttgttttt ttcgatgcga aatcgatggt ttttaaccat tctttcaact
    17341 tacttgtatt acccgaacga atcgcttgaa gggtcaaagg tggtagcgaa aactgataga
    17401 ccatctgtgc ttcattctcg ccattcccaa aataactgat attatctttg tgtggcacat
    17461 ttgtctcagt gatcaatacc gttcctggag ctgcttcatc agcgattgca cggaataatt
    17521 ggatgatttt atgcgtttct ggcaggtgta tacaagaagt tcctagttct ttccataaaa
    17581 atcccacagc gtccaagcga ataaatgaag cgcctttttc taagtaaaac aaaagaatat
    17641 cgatcatttt caataaaact tctggatttt taaaattcaa gtctacttga tcttcgctaa
    17701 aagtcgtcca tacgtgtttt tctttcccgt ttttgtcaat gaacgttgta agtaacgggc
    17761 tggtccttgg tctagtcacg ctgcttgtat ctgtatccag tggcaaatca ataaaaaaat
    17821 ctttgtacgt atcattcaag tttaaatatt cctgaaacca agtactttcg gcagacatat
    17881 gattgcagac aaagtcgaac attaaccgtg tttcgcttct catttcttct atatcttccc
    17941 atgtgcccaa acggtcgttg attgcctgat aatttacaac ggagaaacca tcatcagatg
    18001 tatatgggaa aaatggcaag aaatgtacaa ctctaaatgt atctgccaaa tacttttgat
    18061 aaaatttctt aaatgtacgc aaagttggtt gctgctcttc aaaaaattga tccccataag
    18121 taatcaagaa aatatcttct tcgtcccaat gcagtttgcc tgcctggcct ttcttctgct
    18181 gataaacact gactttttgt tcaatcatag aggcggtttt ctcagacaaa tctgagtcgc
    18241 gataaatcgc ttgtaaaagt tttgtataca tcggattccc ctttcttttt tgggagcgtt
    18301 cccaacgatt tgatcatatt atggaaaacg cttactgtca atagcatttt tttaaataat
    18361 tttgatatac acaaaaatac aaataagaaa gcttatgtat caataattaa tagcttctta
    18421 aaaaaatatg gtctctcatg atcatgcaag ctattcctaa attttcaaaa tcatagacaa
    18481 aaaagaatgt ctattgtact atggaattat tctaaaacgc tcccaaaaag gaggtgcata
    18541 taaaattgaa acctactatt tatgacattg cgaaaaaagc taatgtctca aaatcaactg
    18601 tctccagagt attaaataac cagccaaata tctctgataa agcaaaagca agtgtacttc
    18661 aagcaatcaa agaattgaat tatgaaccga gcaaaattgc aagaggactg tcaggaaatg
    18721 gttttgacgc gattctagca ttatctcatc gtacttctca tacgacctat ggcaatcctt
    18781 ttttttcaga tgttattcaa tccatttctg ccgtttctga acaaaataac ttcgacttga
    18841 ttctacaaac ggcaaaaaca ccagaagaag agctagaaaa atgcgcgaaa aagatccaag
    18901 aaaaaatgat ccgaggaatt attattttaa gctctacgat ggatgaaaca cttctttttg
    18961 aattggatca atacaatatt cctattgtgg taatcggaaa agtcggaaac gactacacga
    19021 acatctactc agtggacacg gataatttcc aggatagtta cgatttggtg gaaacactga
    19081 tcaagaatgg acaccgtcat atcggctgtc tccatgctcc gacagaagtt tatgtatcca
    19141 atgatcgtct caatggttac cgtcaatgct tatttgatca ccacctagat atccgtaatg
    19201 aatggattat agacggcggc tttcgcttag aagatagcat ggcagcaatg aatgatttaa
    19261 tgaagctacg agaaagacca actgcgattt tttctaccga tgcattaaaa tcattgagta
    19321 tttacaaagt ggcagaacaa tctggaaagc agatacctga tgatctttcg atcgcaggat
    19381 tcagtgatcc cttattgtcc tccctatttt cgccacagtt gactcggata gacattccga
    19441 caaatcagtt aggaatcaaa gccacagaac ttttattcca actaatccac caccaatacc
    19501 ctgaagaaaa aagaatcatt ctttcaaccg agattactct cacagattcg atccaacaac
    19561 aagaaatgta gaacagaatt ataaaagaac agaaacactg aggataagtc agttactctg
    19621 ttcttttttc aatcactcat ccctcgttac cccctcccgg tattttagcg aatttttctt
    19681 attttccatc tgttttagat agctgtggat aactataatg tttgtgttaa actatttttt
    19741 ccacagggaa gaaaaaacta tttttaacca cgttgtgtat aagtttttat agttcgtttt
    19801 tttaaacaca tttaccctta tttcaatcca tgttatttgg ggatacttat ctctaaaaca
    19861 taaatagaat aacagacatg tctactggtt ttgttttatt cgaggaatta ttcaataata
    19921 gctagcaaaa tattaaaaat aaccgctcta tcttcccatc aatttatttt gcattcaaaa
    19981 aatgatctat ctttactttt gttgattgac ttctcttgga agatgatcgt tccgatacct
    20041 taattggata cgaaccgatg ttgttaagat cctttcccac ttttcttcta gttagacaaa
    20101 ttattttaga caaaccaaaa aagaggttgt gacaataatt tgtcacaacc tcttttttaa
    20161 aatatctatt ttgtttgatt gttcattgcg tcttctgcag ccatttgcgc ttcaatatct
    20221 gaaatactgt agacattttc gcttgctaca ccgacttctt ttggttccat gtcacgataa
    20281 cgttgcatac cagtaccagc tgggatgatc ttacctataa taacattttc tttcaaacca
    20341 agaagaggat ctttcttacc gcggatcgca gcatccgtca agacacgagt tgtttcttgg
    20401 aatgatgcag cagataagaa actatttgtt tctaatgatg cttttgtgat acctagtaag
    20461 acaggtcgag aagttgcagg aacgcctcct gagactagcg tattgtagtt ttgatcttta
    20521 aagtctgcga tgtctaataa tgtacctggt aagatatctg tatcacctgg atccattaca
    20581 cgaactttac gcagcatttg acgaaccatt acttcgatat gcttgtcgcc gatttctacc
    20641 ccttgcatac ggtaaacacg ttgtacttca cgaagcaagt agttttcaac agataataca
    20701 tcgcgaactt gcaacaattg ttttggatcg attgatcctt ctgtcaatgg tgcaccacgg
    20761 tggatcatat cgccttctgc tactttcata cgtgctgtat aaggaacagt atacgtacgt
    20821 gtatctgttt ttcctttgat tgttacttct ttggtacgtg ttgcaggatc ttctgagata
    20881 tcgatgacat cccctgttac ttctgtgatc actgcttgcc ctttcggatt acgtgcttca
    20941 aagatttctt ggacacgagg aagaccttga gtgatatcgt ctccggcaac cccacctgta
    21001 tggaatgtac gcatcgtcaa ctgagtacca ggttcaccga ttgattgagc ggcgatcgta
    21061 ccaactgctt ctccgacttc aacgtcagag ccagttgcaa ggttgcgtcc gtaacaatgt
    21121 ttgcagacac catgttttgt attacatgtg aagacagaac ggattgtcac tgtttcgaca
    21181 cctgcatcaa cgatttgttt tgcgatgtct tcggaaatca attggtttcc tgcaatgatc
    21241 aattcatttg tttcaggatg acgtacttct ttacgggtgt aacgtcccac taaacgatct
    21301 tctaatgatt cgatgatttc atttccctcg cggatggctt ggatttcaag acctcggtca
    21361 gtaccgcaat cttcttcacg gatgattaca tcttgggcca cgtcaaccag acgacgagtc
    21421 aagtaacctg aatcggcagt cttcaaggct gtatcggtca tccctttacg agccccgtga
    21481 gttgagataa acatttccaa gacggatagt ccttcacgga agttcgaaat gattggcaat
    21541 tccatgattc gaccattagg agcggccatc aaaccacgca ttccggcaag ctgtgtaaag
    21601 ttggaaatgt taccacgagc tccagaatca gacatcatga agattggatt tttcgcttcc
    21661 aaaccttcca tcagtttttg ttggatgctg tcttttgcag cattccaaac agcaatgaca
    21721 cgttcataac gttcgtcatc tgtgatcaga ccacgacgga attgttttgt aatagtttca
    21781 acttgtttat gggcattttc aatgatttct tgtttttcat tcagtacact gatgtcagcg
    21841 atccctactg tgataccggc atgtgtcgaa tgtttgtacc ctaagtcttt catacggtca
    21901 agcatcttag aagtttctgt tactttgaat cgtttgaaga cttctgcgat gatgtttcct
    21961 aagtttttct tcttgaacgg taatactaat tcttgctctt tgatatgtgc aggaatatca
    22021 gtaccagctt caacaaagta tttgtccggt gtttggacgg tcaagttgaa gtctgttggt
    22081 tcattcagat aagggaattc aggaggcatg atctcgttga agatgatctt accaactgtt
    22141 gtgatcatcg tacgttcttt ttgccattca gtaaatggtt tttcacctaa tgagttaggg
    22201 ttcacaccga tacgtgaatg taaatgaaca tatccgttgc gccatgcgat aacggcttcg
    22261 ttttgatctc tgaagaccat tccttcgcct tcgcgtcctt cttcttccat tgtcaagtag
    22321 tagttaccta gaaccatatc ttgggaagga gtaacaactg gtttaccatc tttagggttc
    22381 aagatgtttt gagcagctaa catcaacata cgtgcttctg cttgtgcttc ttcattcaat
    22441 ggaacgtgga cagccatttg gtctccatcg aaatcggcat tataagcttc acatacaagt
    22501 ggatgcaaac ggattgcgcg tccttgtact aaaactggtt cgaatgcttg gataccaagt
    22561 cgttgaagcg taggtgcccg gttcagcaat actgggtgtt ccttgattac gtcttctaaa
    22621 acatcccata cttcgtcttc ttgacgttcg attttacgtt tcgcattttt gatgtttgaa
    22681 gctagttcgc gttgaacaag ctcacgcatc acgaatggtt tgaataattc aatggccatt
    22741 tcttttggaa gtccacattg gtacatcttc aagaaaggtc caacaacgat aacggaacga
    22801 cctgagtagt cgacacgttt acctagcaag ttttgacgga aacgaccttg tttccctttc
    22861 aacatatgag aaagagattt caatgggcgg ttacctggtc cagtaactgg acgtccgcga
    22921 cgaccgttat cgatcaatgc atctactgct tcttgcagca tacgtttttc attttgaacg
    22981 atgattccag gtgcattcaa gtctaacaaa cgtttcaaac ggttattacg gttgattaca
    23041 cgacggtaaa gatcattcaa gtcacttgtt gcaaaacgtc caccttctaa ttgaaccatt
    23101 gggcgtaaat ctggtggaat aacagggata acgtccataa ccatccagct tggttgattt
    23161 cctgaagcgc ggaatgcttc taaaatatcc aaacggcgga ttgcacgtgt acgtttttgt
    23221 ccttgtgcag atttcaattc ttctttcaat tcggctgctt ctgcatctag atcaacatta
    23281 tcaagtaatt gtttaatcgc ttctgcaccc atagcagctt ggaattcttg tccatattgt
    23341 tcacgttttt cgcgatattc gcgttctgtc aacaattgtt ttttctctaa attcgtattt
    23401 cctggttcga tgactacata tgaagcaaaa taaatgattt cttccaaagc gcgtgggctc
    23461 atgtctaata caagacccat acgtgaagga atccctttaa agtaccagat atgtgaaaca
    23521 ggagctgcta attcaatatg tcccatgcgt tcacggcgaa ctttcgaacg agtaacttcc
    23581 actccgcatc gatcacaaac gattccttta taacggatac gtttgtattt tccgcaggca
    23641 cattcccagt cttttgtagg accaaaaatc cgctcacaga acaacccttc gcgttcaggt
    23701 tttaaggtac gatagttaat cgtctcaggt ttttttactt caccgtaaga ccagcttctg
    23761 attttttcgg gagaagccaa acctatttgc atactttcga atttatttac atcgatcaaa
    23821 aggggttccc tccatttcat ttaatggcaa accgcaagac ggctgatctt cgaccgcctt
    23881 gcagctcata gaccggatta gtctaaatta tcttctgcgg tttcaaagtt ttggatgaca
    23941 tcgtcttttt catcttccac ttgttcagca gctttctttt ctaattcttt ggcagtttgc
    24001 tgttcggcaa attttgtcaa agcatcaaca gtgatcaaat cgtcgtcttc atcgtccatg
    24061 tcgcgaagtt cgatctctgt ttcttcgata tccaagacac gcatgtccag acctagtgat
    24121 tgcaattctt tcaccaatac gcggaatgat tccggtacac ctggttttgg aattggttca
    24181 cccttgacga ttgcttcgta tgttttcaca cgtccgacta cgtcatctga tttgtatgtc
    24241 aagatttctt gtaaagtata agccgcaccg taagcttcca gtgcccatac ttccatttca
    24301 ccaaaacgtt gtccaccaaa ttgagcttta cctcccaatg gttgttgtgt aaccaatgag
    24361 taaggtccga ttgaacgtgc atgcaatttg tcatcgacca tgtgagcaag tttgatcatg
    24421 tacatcacac cgacagacac acgtccatca aatggttcac ctgtacgtcc gtcgtacaga
    24481 acagttttcg catcgctggc cataccggct tcacgaactg tttcccatac gtcttcatca
    24541 cttgcaccat caaatactgg cgttgctacg tggataccta actgacgtgc agccataccc
    24601 aagtgtaatt ccaatacttg cccgatgttc atacgtgaag gcacccctaa aggattcagc
    24661 atgatatcga ttggtgttcc atcaggtagg aatggcatat cttcttccgg cataatacgg
    24721 gatacaaccc ctttatttcc gtgacgaccg gccattttat ccccttcatg gattttacgt
    24781 ttttgaacga tataaacacg aactaacatg tttacgcctg gtgacaattc atctccagct
    24841 tcacgagtaa agattttcac atcgtgaacg ataccgccgc cgccatgtgg tacacgtaat
    24901 gatgtatcgc ggacttcacg agctttttcg ccaaagatcg catgcaatag acgttcttct
    24961 gcagataatt ctgttacccc ttttggcgtg actttcccta caagcaaatc gccatcttgg
    25021 acttctgcac caatacggat gatacccatt tcgtctaaat cttttaatgc gtcttccccg
    25081 acattaggga tttcgcgtgt gatttcttca ggtcctaatt ttgtatcgcg ggcttctgat
    25141 tcatattctt caatatggat agaagtgtat acgtcatctt ttaccaaacg acggctcatg
    25201 atgatcgcat cttcatagtt gtacccttcc caagtcatga aaccaactaa aacgttttgg
    25261 cctagagcca tttcgccttg ttccatagat ggtccgtctg ccagagtatc ccctttttcg
    25321 actttctcac ccaaatgaac gattggacgt tggttgtagc ttgttccgga gtttgaacga
    25381 cggaattttg ttacatcgta tttatctaat gcaccgttgt cgcggcgtac gcggatttca
    25441 gaagcatcaa cgtattcaac gacaccgtca tgtttacata acaaagcagc tcctgagtca
    25501 tgcgctgatt tgtattccat accagtacca acccatggtg cttgaggatt gatcaacggc
    25561 actgcttgac gctgcatgtt ggcacccatc aaggcacggt tggagtcatc gttttccaag
    25621 aaaggaatac atgctgtcgc aacggcaact acttgtttag gtgatacgtc catgtagtct
    25681 actttatcaa tagatacttc taagttttca ctttgagcac gagccataac aacgtcttga
    25741 gcaaatgttc catcttcatt caatgggctg tttgcttggg ctacgatgta atggtcttcg
    25801 atatcagctg tcaagtagtc gatttggtca gtaacacgac ctgtttcacg gtctacacga
    25861 cgataaggtg tttcgataaa tccgaattta tttactttcg cataactgga caaactattg
    25921 atcaacccga tgtttgggcc ttcaggagtt tcgatcggac acatacggcc atagtgggag
    25981 tagtgaacgt cacgaacttc ataaccggcg cggtcacgag tcaatccgcc aggtcctaag
    26041 gctgaaagac gacgtttgtg tgtcaattcg ccaagagggt ttgtttgatc catgaactgt
    26101 gacaactgag aagaaccaaa gaattctttg atacttgcca caactggacg gatattgatc
    26161 aattgttgag gtgtcaatgt ttcagtatct tggatcgaca ttctttcacg aaccacacgt
    26221 tccatacgtg ctagcccgat acggaattgg ttttgtaaaa gttcaccgac tgaacggata
    26281 cgacgatttc ctaagtggtc gatatcatcg acattgccga taccttccat caagttcaag
    26341 aaatagctca ttgaagcaat gatgtcggct ggacgaaccg tttttacagc tgcttctgga
    26401 tagccgtttc cgatcacgtt tacttcacgt tcagggtctc ttggtgaaaa tactttgatg
    26461 acttggacag tcattggatc tgtgaccaca ccgtcttctg atggatagta agtcactgaa
    26521 tttaggccat tatcaatgaa aggagctagt gtttccatga cttggtgagt cagaactgtt
    26581 cctttttcaa cgatgatctc gcctgtttct ggatcaacta atgtttcagc taatgtcaga
    26641 ttcaatagac gtgttttcaa atctaatttt ttgtttactt tgtaacgacc aacgtttgct
    26701 aagtcgtaac gttttggatc gaagaaacga gcattcagca agttacgtga gctgtctgct
    26761 gtttttggtt cgcctggacg caaacgttcg taaacgtctt tcaaaccttc ttctgttctt
    26821 gagtcgcttg cgtttttatg caagtctttt tcgactgtat tgcgtaatgt ttcgctgtcg
    26881 ccgaagattt caaagatcgt atcatcggaa ccaaatccta acgcacgaac taatacggtt
    26941 aaaggaatct tacgtgtacg gtcgatacgt acataagaga tgtcttttgc atcagtttcc
    27001 atttctagcc aagctccacg gttagggata acagtagaac cgaatccttc ttttccgttt
    27061 ttgtcgactt ttccatggaa atagacacct ggtgaacgga ctaactgaga aacgattact
    27121 cgctctgccc cgttgatgat gaatgttcct tgttctgtca ttaatgggaa atcaccgaag
    27181 aatacttctt gtgctttgat ttcgccagtc tcgcggtttg tcaaacgcaa tgttacatgt
    27241 aatggtgcag agtagtttgc atcatgcgca cgagcttctg ctactgtata ctttggttct
    27301 ttcaattcat aatctacaaa ttccaaagat agattgccat tgaagtcatc gattggcaaa
    27361 atgtcttcaa acatttctct aagtccctca tctaagaacc attggtaaga gtctgtttga
    27421 atttcgatca aatttggtaa ttccaacact tcactgattc ttgcgaaact tctacgttcg
    27481 cgatgttttc cgtattttac tacgtgtcca gccaagctct tcacccctct aaaatgtcat
    27541 tcaaaaagac tacccgatca attgttacaa taatatttca aatattattt ttaggtaaca
    27601 acagacattt tttgcggctt ttaggcaaaa aaaaaccaaa aatagaacat tctcttgaaa
    27661 ccttacttcg tgagcttcta cttttgtttc cttatattaa agccgaaacg gacaatattt
    27721 cgtttctgcc ttttcttcat cagatagttt ttgcaacttc atatactaca cttattttct
    27781 ataaaagtca acacttcctg gcgtttcttc tgctttttcg caacattttc ttaatgtttt
    27841 caattttatg ttttcaatta tctgacaatt cttaattgac agaccttttt atctcggtta
    27901 tactttagaa aattcatttt gtggaggaac ttctatgcga accaacatca accaaatcgc
    27961 accaacacaa ctgaataact tttactttag ctactttaga taggtttgta ttcatcatga
    28021 tggatacaaa cgttaaacga cagtttgtat ctatgatggc taaagaactt tgtattgcag
    28081 caaaaagcca cgtagacaaa atgctaagtg gctgagcagt gaaaatacaa agtaaaagta
    28141 tcaagcattc tactttttca atttattttc cgacttttca atccatttgg acattttgtc
    28201 tgaatggatt ttttgtttcc caaaattaag gaggaaaaag aaatgaaaaa aaatataatc
    28261 atcggattag gcgctgccct gctactagct ttaggaggtt gcgcacaaaa agacaattcc
    28321 gcagaggcat cagatggaca aaaaacaatt ggcgtattgc aacttgtaca acataactcg
    28381 ttagatgctg cctataaagg attcaaagaa ggattagcag aagaagggta taaagaaggc
    28441 gataatctaa caatcgatta tcaaaatgcc caaaacaatc aagacaacct gaaaagtatg
    28501 agtgagaaac tcgtcaaaga agatcctgat ttgctacttg gaatcgccac tccagcagca
    28561 caatctttat taaatgaaac aaccactatc ccgatgactg tcacagcggt cacagacttg
    28621 gaacaagccc atttagtcac atccaatgaa aaacctggcg gcaatgtgac tggcacgaca
    28681 gatatggtgc caatcgaaaa acaaatcggc ttactgttaa atatcgtgcc agacgccaaa
    28741 acaatcggca tcatgtataa caacggtgaa gcaaactcta agatccaagc tgatttggca
    28801 gaaaaagaat tgaaaaaagc gggcgtcaaa gtcaaagtac tgacagcaaa ttccacaaat
    28861 gatgtccaac aagtgacgaa aagtctagct aaagatgtag atggaatcta tattcctact
    28921 gataacacgt tcgcaagtgc ggcaagtgtt gtcggtgaag tcgcaaaaga aacaaaaaca
    28981 cctgtcgttg ctggcgcagt tgatcaagtc aaagacggcg gtctagcaac agtaggaatc
    29041 gactacgaaa gcttgggtaa acaaacaggg atcatggcag caaaaatctt agacggcaag
    29101 aatccaagtg aacttcctgt cgaaacagcg aatgatttgg aactgtatgt caataaagac
    29161 atggccaaag cattaggcat caatccagac agcatcaaag aagctgaata aaacacagga
    29221 ggattctcat gacaaatttg ctctctcttt tcttatcatc tgcatcgcaa ggggtcttat
    29281 gggccctact tgcgattggt gtatacgtca ctttccgtat cttggacatt gccgacttga
    29341 ctgctgaagg aagctttcct ttaggtgcag ggatcgcggc agtaagtatt acaaatggct
    29401 atcatccatt gacagcttgt cttctaggct ttttaggggg tgcggcagca gggcttgttt
    29461 ccggactgct tcacacaaaa ttgaaaattc ccgcccttct tgcagggatc atcaccatga
    29521 ctggtttata ttctgtcaca tcccgtgtga tgggtgcgcc gaacatctct ttattgggtc
    29581 agccaacgat ctttcattgg gtcgagtcat tcggcataac aaaagacaac gctgtactga
    29641 tcgtcggtct gctttttgct ttgatcgttg tatgtttact tgttttgttc ttacgtacag
    29701 aaacaggact tgctattcgt gcaactggcg ataatctggc aatgagcgaa gcaaacggga
    29761 tcaacacaga taatatgaag atcatcggtt atatgatttc taatggatgc attgctcttt
    29821 ctggctcctt gcttgcgcaa aacaacggct ttgctgattt aaattctggt attggaacga
    29881 tcgtaattgg acttgcttct attatcatcg cagaagtttt gttacgtaac cagccactat
    29941 ttctccgtct cttgactgtc gttttaggtg cggtcattta tcgttttatt ttagcgttag
    30001 tcttcgaact gaatgttgaa ccaagtgatt cgaaacttgc ttctgctttc gtgctagtga
    30061 tctgtctttc tttcccgcag atccagcaaa aattccattt acctcaaaaa aagaaaggcg
    30121 gtagtgtcaa atgaatgacg tcttaacatt ggaaaatctc catcatacct ttgaaaaagg
    30181 aacgatcaac gaaaatcatg tcctgaaagg cattaatctt tccttagatc ccggcgactt
    30241 cgtgacgatc atcggtggaa atggggctgg taaatctact ttgctgaaca gcatcgccgg
    30301 tacattttct gtcgatcagg gtcaaatttt attgaatgga aaaaatatca cgaaaaaatc
    30361 tgttgtcgaa cgttcgaaat ccatcagtcg cgtattccaa gatcctaaac tagggacagc
    30421 cgttcgttta acggtagaag aaaatcttgc tcttgctatg aaacgaggaa aaaaacgcgg
    30481 cttcttccgt ggtgtcaaac cacaagaccg aacatttttc aaagaccagc tggttcggct
    30541 aaatttgggg ttggaaaatc gtttatcctc agaaatcggt ttgctgtctg gaggacaacg
    30601 tcaagctatt acgttattga tggccacatt acagcgtcca gaactgatct tattagatga
    30661 acacactgca gcgcttgacc ctgctacttc caaaacggtg atggaattga cagaaaagct
    30721 gatcactgag cagaatctca ctgcttttat ggtcactcat gacatggaag acgcgatccg
    30781 ctatgggagt cgactgatca tgctgcatca aggaaggatc gtcgtagata tccagcaaga
    30841 ggaaaaacag cagctcacag tgccccaatt attagcgttg ttcaaacaaa acagcggcac
    30901 ggcattaaaa gacgatcaag tacttttatc ttcataatca gaaagaggat gggacaaaaa
    30961 atgaacgccc accctcttct tttttctctt tccttctttc gtttttgctt tatgtatcaa
    31021 ccgttgtttc ttcgaaaact attttgttct ctccttcttc ctgatagaat ggatgggaaa
    31081 ggagcttgat gccatgacaa cgaaagacaa ggtacttcaa ttattgaaaa agacaccaga
    31141 ttttctttcc ggagaaaagt tggcacagca actggaagtt tcccggacaa gtatttggaa
    31201 agccatcaaa gaattggaaa aggcaggcta tcgttttgag cacgggccta cagggtatcg
    31261 ttacctgccc tctgacgtac tagatgcaaa agaaatccag caagagctac ggtcgtttat
    31321 tcctgatttg aaagtacaaa cgatgttgac ttctgaatct acgatgaaag atgtcaaact
    31381 ggcagcagct caaggagatt ctgtttcgaa gttatttgtt gctgatacac aagaacaagc
    31441 tcgtggacgt tttggacggc cttttttcgc tgaacctgga cgaggaatct acatgagtct
    31501 cttgctccat ccagacaaac ggtttgatga attgcctcaa tatacgatca tcgcagccag
    31561 tgcctgtgtg aaagccattg aatctgtaac cggtcgttct gcttcgatca aatgggtcaa
    31621 tgatatctac atggatggaa aaaaagtctg tgggatttta tctgaagcag tcagtgatat
    31681 ggaaagcggc aggatcagtc atatcatcat tgggatcggg cttaattttt ctgttcctca
    31741 aaatcatttt ccagctgatc tccaagaaaa agccatctct gtctttcccg acggacaagc
    31801 tagtgtcaca aggaatcagc taatcagcaa aatctggcaa gaattgttca cttatctttc
    31861 tcacccagaa aaagcgttgg acttctataa agaaaaatca ttcgtattgg gaaaaaccgt
    31921 gtcgttcaat caaaaaggaa cgacttatgt cggtcttgca aaagaaatcg gtgatcatgg
    31981 cgaactgatc gtccaaacaa atgaacagaa gatgagtttg tcttctggcg aaatcagtct
    32041 cacatctatc cgtacgcaat aattatcaga tttcacaaaa ggacacctga tctcatacag
    32101 tgtccttttt tctgaattct cttcaaaaga ctctccttat tctatcagct gacagtttct
    32161 ctatcctcta gaaaaaggta ttctaaaaat atctataaag catggtacac tttctttaaa
    32221 tgaatcaatg caaaaaacaa gagagtcatc agtttacacg atcaaaagag atagcaggcc
    32281 gcttcctttt tcaatcttat agatagacca tgggtaacga taaaaacaaa aaagaacttg
    32341 cgacttattt tgccgtaagt tcttttttag ctagtgcctt aatctcttaa tcccaacaaa
    32401 cgtctcgcat agatcacctg accagagtgc atagcagcat catcaataat agaaacgaga
    32461 cgcattcctc gtgtgactgg tggtgtccag ttctcgtcaa ttacttcatc tagctcggaa
    32521 ggttccagcc ttcttaagta agaaatagca aaatcagcag ctcctcgcaa gtatcctagc
    32581 agatcttcta tttgattagc ttggatctgc tttgcttgtt ccagactatg cttccaatct
    32641 tgttcgttat ctgctatcgt catcggaaac cggctttccc agtcttgact atgccaaatt
    32701 ggagaagttc ctgctaaatc agcaatctgg aaatctaatt ctcgagcggt atgccaagcc
    32761 agccaagtca ttgatttgat ctgcggggct ctatcttcca ctggaaaacg attggcctcc
    32821 tcaatagaga cgccttcaaa caaacgttca aaccgatcgt atgcccgatc cacactctcg
    32881 catagcatat ctaaataatc catttatttt cctccttttt atttatccta cttacttttt
    32941 cctaattcta ggatacctac ttatatgtta ctacacaccc ttatttttta cactttaaca
    33001 gtcaccatca tcaatagttt ggagattgaa agaaaaagat agtgtcgaac tatcaattga
    33061 acaagaagca acaatatgaa cgtttcatta atattcttta aataaacaat gatttttcaa
    33121 acgaacagtt taagactagg agaaaaagag tataatagga aaatgtttga aaggaaagga
    33181 aggggaaaaa tgaaaaaaag ggcaagttta ctagtcctag ttgctttgtt gttcaatatc
    33241 ttttcgtatg gtgtggccgt ttcagcggaa acatacattc ctaaagaccc tgggaatatt
    33301 tataatgatc taactggtaa ctatggcttg ttaggaattg ctagtcaatt ccacgtattt
    33361 gctaaaaata agacaactgt gaatgcacat acagacggaa atatagcaac acatgaactt
    33421 gatgcaaaaa ataattttgg tacagagctt catgaaggtt tattgaacca agaaataaat
    33481 tatatacaaa aaactgattc tttcgctaat tcttcaggca tacctacagg tagcacaaga
    33541 atgaataaat ttgtcgttgg cagtaacgtg gatgtagcac ttcaagatgg tcatcctaca
    33601 gtcaatggat ccaggcttga tcacttaact ccagatgatt tttataaaga tcgtgctggg
    33661 catgaatata ttgatttcga gcaggaattt gctaaattga atgtagcagc aaatgatctt
    33721 gcaacgatca ctccagctaa aacatatact gctgcagatt ttccagacat gaacaatcgg
    33781 acaatcgatt taactgattt atcagatacc gggtttttat tggtaaatat cgatgcagaa
    33841 gtgttaacga tgaatacgcc acttcaaatc attaatccaa atgatcaagt agttgtattt
    33901 aatgtcatca acagttcttc tgcattgaac gtacagtcac caatcaaata caatgatcgt
    33961 tcaaatcatg aaacagaaga tttttcggat gcgaatatct cttggaattt tggaaatgag
    34021 atgactgatt tgacaatcag tgctcctttt caaggaacaa tccttgcgcc taatgctact
    34081 atccgcatta ctcaaaacca agacggaacg attataggaa caaatgtgat tttagatgca
    34141 gctaccaatc gttgggatcc taacgaaata tttatcagcg acaatggtac agatacgaca
    34201 gacacaagcg acagtacaga tacatctacc acaagtgata gctcagattc atcaacttca
    34261 agcgacagta cagatactac ctctacc
//
LOCUS       DAZKLD010000012        33264 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.012, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000012 DAZKLD010000000
VERSION     DAZKLD010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 33264)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33264)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33264
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.012"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            52..2649
                     /gene="adhE"
                     /locus_tag="ACG8KR_000510"
                     /gene_synonym="adhC"
     CDS             52..2649
                     /gene="adhE"
                     /locus_tag="ACG8KR_000510"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006066 - alcohol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HFQ0199912.1"
                     /translation="MAKVIEKEKTKTIDVQAMIDDLAEKANAALKEMESFDQEKVDHI
                     VHEMAMAALDQHMPLAKMAVEETGRGVYEDKAIKNMYASEYIWHNIKHDKTVGVINED
                     VQKGLIEIAEPVGVVCGVTPTTNPTSTTIFKSMIALKTRNPIVFAFHPSAQKSSAEAA
                     RVVRDAAIAAGAPENCIQWIEHPSIEATSMLMNHPGIAIVLATGGAGMVKSAYSTGKP
                     ALGVGPGNVPAYIEKTAKIKRAVNDLIVSKTFDNGMICASEQAVIVDKEIYAAVKAEF
                     QAHQVYIVKPDELQKLEDAVMNEGKYAVNPSIVGHSAMEIAKLAGISVPKGTKMLIAE
                     LEGVGPDYPLSREKLSPVLAMIKANNTDHAFDLCEGMLNLGGLGHTAVIHSENEELHV
                     KFGLRMKACRILVNTPSAEGGIGDIYNEMIPSLTLGCGSYGKNSVSRNVSAVNLINVK
                     TVAKRRNNMQWFKLPPKIFFEKNSLLYLEKMENVERVMIVCDPGMVQFGYCDTVREVL
                     ARRKNDVKIEVFSDVEPNPSTNTVYAGTKLMADFKPDTVIALGGGSAMDAAKGMWMFY
                     EHPDTSFFGAKQKFLDIRKRTYKIDKPEKTQFVCIPTTSGTGSEVTPFAVITDSETHV
                     KYPLADYALTPDVAIVDPQFVMSVPASVTADTGMDVLTHAIESYVSVMASDYTRGLSL
                     QAIKLVFDYLEKSVKTPDMESREKMHNASTMAGMAFANAFLGICHSVAHKIGGEYGIP
                     HGRTNAILLPHIIRYNAKDPQKHAMFPKYDYFRADTDYADIAKFLGLKGETTEELVEA
                     LATAVYELGKSVGINMSLKAQGVTQETLDTTVDRMAELAYEDQCTTANPKEPLISELK
                     QIIIDAYNG"
     gene            2878..3984
                     /locus_tag="ACG8KR_000511"
     CDS             2878..3984
                     /locus_tag="ACG8KR_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFQ0199913.1"
                     /translation="MIIKEEIFVPKELVKEKIDLLVENLTTIKDDNGEFLLDFDGLKV
                     DDKSWTVWNWPQGVGLYGIYKNYQMTKNPRAYQVVNEWFEDRMEEGAPPKNVNTMAPL
                     LTMAYLYEDTKDSRYLPYLEQWAEWVMNDMPRTNEDGLQHATYGPENKNQLWDDTLMM
                     TVLPLAKIGKLLNRPEYLEEARYQFMIHIKYLMDKRTGLWYHGWTFEGNHNYAEAFWA
                     RGNCWLTIAIPEIIEILELSKEDALRKLLIETLEAQVRALKTYQSESGLWHTLLDDPS
                     SYVESSATAGFAYGILKAVHKRYLPQEYKEVAYRAIQGLLEQIDEKGEVQNVSIGTGM
                     GDSLDFYRNIGITAMPYGQSLTVLCLTELLVSYC"
     gene            4001..5029
                     /locus_tag="ACG8KR_000512"
     CDS             4001..5029
                     /locus_tag="ACG8KR_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HFQ0199914.1"
                     /translation="MKKTSLLFAASSLSLGLLGLSGCGNNSSVATVADADVNEIVTLR
                     FAYASNSQPVIDAMNEFGRLVKEKTDGSVQIQYFPDGQLGGERELIELTQSGAVDFTK
                     VSASALESFSKDYSIFSVPYLFNDEEHFFKVMDNEEIMEPIYQSTKNLGFTGLTYYDS
                     GQRSFYMVDGPIHTPEDLKGKKIRVMQSETAIKMVELLGGSPVPMGSDEVYTSLQSNL
                     INGSENNEFVLYTAGHGGVAKYYSYDEHTRVPDIIIMNDAIKEQLTDEQEKAIEEAAK
                     ESTVFEIEAFAKAIEEEKALAQEEHGVQFNDVDNTPFREAVAPLHDIFKNDPTYKDLY
                     QKIQEQGA"
     gene            5035..5514
                     /locus_tag="ACG8KR_000513"
     CDS             5035..5514
                     /locus_tag="ACG8KR_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HFQ0199915.1"
                     /translation="MKMILNKVLEILGAVLLVGMVAVVLWQVFSRTVLGNPNTVTEEL
                     VRFGLVWFAMLSSAYVVGQKAHLAVTVLSEKLTGKKSKILEVVVQSLFLVFAAMIMVY
                     GGWNAVTLTMGQISPSLSIPMGYVYLSVPVSGVLIIIYSLINLIDTLSNKPAIQEKI"
     gene            5530..6825
                     /locus_tag="ACG8KR_000514"
     CDS             5530..6825
                     /locus_tag="ACG8KR_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HFQ0199916.1"
                     /translation="MAIEAGLVLFFVFVVLLVLGMPIAISIAFSSMMTLLLVIPFDVS
                     AFSSAQKMVGSINSFSLIAIPFFVLSGIIMNNGGIAKKLVDFAMLFVGRVPGALAYTN
                     VLGNALFGSISSSAIAASTAIGGVLIPQQVEEGYDRKFATAVNIASAPTGMVIPPSTA
                     FIMYSLVAGGASISALFMGGYLVGALWALGIMLVAFIVAKKNKYPVVEKGQMAHAGKI
                     IKEAIPSLLLIVIIIGGILTGIFTAVEASAIAVIYSLLIAMFYYKTVKLKDIPNMLFQ
                     SVAMSGTIMFLLATSSMMSFAMAFTGIPQAISSLIMGVTDNKFIILLLVNLVLLLVGM
                     FMDVAPAILIFTPIFLPIVTSVGVDPVHYGLFSIMNLCVGSITPPVGTGLYVGASVGG
                     VKAEAMLKPLLPFYGVILVVLFLITYFPQIVMWLPNMLS"
     gene            6836..7660
                     /locus_tag="ACG8KR_000515"
     CDS             6836..7660
                     /locus_tag="ACG8KR_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFQ0199917.1"
                     /translation="MLNITIRGHDLSEVQTIEDLAEKTKEQGIHTLQLALGLSFPEIP
                     SGANEINSGMGNYVKRVLEKQEVTVGILSCYINMIHPDLTIRQELLTKFEQYLRYASS
                     FGASMVASETGCVLPEIQYTEENFTDEAFAEAVSVIRRLVKAGEKYQMMVGIEPGLNH
                     PVYSLARVEQLIQAVDSDYLGIILDPTNLITSSDYQEQVQLVEEAFERFGEKICAVHL
                     KDFRVEQEKIVPVNLGDGVIEYTKIKEIIMKNRPYLYVVLEETKDDGIRYGRSLLE"
     gene            7825..8724
                     /locus_tag="ACG8KR_000516"
     CDS             7825..8724
                     /locus_tag="ACG8KR_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0199918.1"
                     /translation="MLQKKISGEQVNTMVSDQYEIYHVKDVVSQNKTIYHHHDFYEIH
                     ATLKGEATFYLNGRQFTIKAGSVLLIHSNDLHRIVRQSSDVFERVYIFVTPSFLESRS
                     TPWSNLSACFQPMGEKRSRVLQIEQEVLKQKLSFIDHPPDKKIYGEDIKYEQALVEYL
                     IFLNQVVQKEESAQEQNAVVPNERVEQMLQYISKNLSHPLSLEEMEKQFFVSKYHITR
                     EFKKYTGFTFHQYVMKKKLIYAKHLLREYRSSSAVYSKSGFSSYPHFLKAFKREFGIT
                     PKEFLKRDTQKQLVHYEHFEDEQ"
     gene            complement(8850..9719)
                     /locus_tag="ACG8KR_000517"
     CDS             complement(8850..9719)
                     /locus_tag="ACG8KR_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFQ0199919.1"
                     /translation="MDYWEKANHSWSEDSKRVILTPTQRSRNLFFYIQEIGYFKAPIP
                     YYTERAHLPSYLIKFTLSGKGSLTYNDNQQQLKAGDLFFIDCQEYQRYQTISEEPWEM
                     DWIHLNGQNVQSFYQEFTKDGHNTFHSTGDPSENKIHHLITELIHMQQGTNARTDFQS
                     SVAIHELLNELILQKYQLDFEEEDIPAYVYKLKQYLDEHFKETITLDQLEQQFLLNKY
                     QLSKDFSKFIGSPPIDYLISKKLSYAKDLLRYTEFTVRMISEEIGIENFAYFSRLFKA
                     KTGLSPRDFRKFS"
     gene            9872..11332
                     /gene="rhaB"
                     /locus_tag="ACG8KR_000518"
     CDS             9872..11332
                     /gene="rhaB"
                     /locus_tag="ACG8KR_000518"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008993 - rhamnulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HFQ0199920.1"
                     /translation="MNPTFVAIDIGASSGRLMKSTRDAKGSLILEEVHRFKNGFRNKD
                     GHDRWKIDYLIKEILIGFEKLKQTGIDRLFAGIDTWAVDYCLIDEQGDRINDPIAYRD
                     KRTNGAVEQFSEIYSLDKLYEQTGIQIQPFNTLFQLFAEEKENLAKAHKLLLIPDYLG
                     YVFTGNMVTEKTNASTMQLLNAGTKEWEIELLEKIGVSKELFPPLTEPGTILGDLQKE
                     QFSKYDLPDVTFITVASHDTASAVLGTPGQGERWGYISSGTWSLLGIETKIANVSRNA
                     FSENYTNEWGAHNTVRFLKNIMGMWLIQEVARMQEYEYSYAELAELAAKEKPLQQVME
                     INDPRFLNPPNMIEEIQQYCFETEQPVPVSSGAIARCVYDNLALCYAQELIKVEHLTG
                     TKLEKLHIVGGGSNNRLLNQLTADYAGIIVEAGPSEATAIGNVAMQMLSTGQFSNIEQ
                     ARNVIKQSFPCETFYPDENKQNTMEWYVNKIKGVIV"
     gene            11329..12585
                     /gene="rhaA"
                     /locus_tag="ACG8KR_000519"
     CDS             11329..12585
                     /gene="rhaA"
                     /locus_tag="ACG8KR_000519"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008740 - L-rhamnose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HFQ0199921.1"
                     /translation="MNTIERNYEQAKEKYATIGVDTDAVLEKIQDIKISMHCWQGDDV
                     KGFLTPDGELTGGIMATGNFPGAARTPEELRQDLEKAYSLIPGKHKLNLHAIYLDTEE
                     SVDLNEIEPKHFEKWVEWAKKEEIGLDFNPTFFSHPMMKDGFTLAHPDKEVREYWIEH
                     GKRSRKIAEYFGKETGQTSINNFWVPDGMKDNPIDRFTPRERLMESLDEIFTEELDER
                     YVQDAVESKLFGLGAEAYTVGSHEFYMGYGLTRDKLVCLDAGHFHPTEVISNKLSSLA
                     LFSKGIMLHVSRPVRWDSDHVVLMDDELQEIAKELVRNELLEKTNIGLDFFDATINRI
                     AAWVIGTRNTQKALLKAMLEPVERLKEMELAFDFTSRMAYTEELKDFPYADVWNYFCY
                     KNQVPVGLDWLEEVQEYQKDVLELRK"
     gene            12599..13444
                     /gene="rhaD"
                     /locus_tag="ACG8KR_000520"
     CDS             12599..13444
                     /gene="rhaD"
                     /locus_tag="ACG8KR_000520"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008994 - rhamnulose-1-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HFQ0199922.1"
                     /translation="MKKIDILQAPYVKEMIETTSNLYRLGWDERNGGNISYLLKEEEI
                     TPFLDPEKVIRRISMIFDASRLAGCYFIVTGSGRYFKNVASEPAENLGIVRVAEDGKT
                     LELLWGLENECLPTSELPSHFMSHIARLEVDPDNRIVMHCHASHLLAMSFTHELDERS
                     FSRTLWQMCTECLVVFPEGISIIPWMVPGTNEIGEATAEKMKETRLVLWPQHGIYGSG
                     KDMDEVFGLIETAEKAAEVYTYVKAQGPILQTITDENLWHLADAFGVTPKAGYLEEVH
                     TKAGV"
     gene            13446..13757
                     /gene="rhaM"
                     /locus_tag="ACG8KR_000521"
     CDS             13446..13757
                     /gene="rhaM"
                     /locus_tag="ACG8KR_000521"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0062192 - L-rhamnose mutarotase activity
                     [Evidence IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HFQ0199923.1"
                     /translation="MVKKAFCMKVYPEKQEEYKKRHDELWPEMRTALREHGVIDYSIY
                     LDPSTSLLFGYLVIEDEKLWEKMANTSINQKWWKYMEDIMETNPDASPVSWELMPVFE
                     L"
     gene            13839..14990
                     /gene="fucO"
                     /locus_tag="ACG8KR_000522"
     CDS             13839..14990
                     /gene="fucO"
                     /locus_tag="ACG8KR_000522"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008912 - lactaldehyde reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HFQ0199924.1"
                     /translation="MSNRMILNETSYFGKGAIESIVPEFQQRGFGKAAVITDKGLIEH
                     GIATKVTELLDKAAIAYALYDGIVPNPTIQNVKDGVAFVKEAEADCLIAIGGGSPIDT
                     AKAIGIILTNPEFSDVISLEGVADTKNPCLPILAIPTTSGTAAEVTINYVITDEVNHR
                     KFVCVDPHDIPIVAFIDSDMMMGMPKKLAASTGMDAMTHAIEGFITKGAWEMTDMLHL
                     KAIEIIGHSLEASVDGDQNGREKMALGQYIAGMGFSNVGLGLVHGMAHPLSAWYNIPH
                     GVACAALLPTVMKYNKEYTGEKYREIALVLGIKGAAEMSLEDVREAACGEIDRLSKAV
                     GIPETISELGVKEADIPAIAEDALRDVCTPGNPRETTVEEIIALYQSLM"
     gene            15117..15875
                     /locus_tag="ACG8KR_000523"
     CDS             15117..15875
                     /locus_tag="ACG8KR_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HFQ0199925.1"
                     /translation="MENKKPYGTVLIKASKILDYLAENQDVSLQTIAKGVGMTSSTVL
                     KILDTLLMIGYVNKNSEKNYRLGAKLIRYANKNIEQIDLVELTLPFLEQLQQKIDETI
                     HLGVLDNNEILYVNKLDPKHQTIRMSSKIGITRPLYNSAMGKAVLAEFSEEQYTDYLE
                     KHPLIPYTEYTITNALRLKKEIKTVKETHIAFDDEEIERDIFCIGASIVKNEQIIGAF
                     SVSMPKYRLTDETKEQIISALKQTKSEIEQRIQK"
     gene            15975..16619
                     /locus_tag="ACG8KR_000524"
     CDS             15975..16619
                     /locus_tag="ACG8KR_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HFQ0199926.1"
                     /translation="MKKIDILSRLKNAGVIAVVRGKSKEEALNACHAIIKGGLTGIEL
                     TFTVPQADQVIKELLSFYKDQPEIVIGAGTVLDAVTARLAILAGAEYIVSPSFDQETA
                     EMCNLYQIPYLPGCMTITEIKTALKSGVDIVKLFPGSAYGPSIISAFKAPMPQVNIMP
                     TGGVSLDNMKEWFDAGVVTVGVGGNLLAPAATGDFDKVTEVAQQYAAKMKEIKR"
     gene            16626..17633
                     /locus_tag="ACG8KR_000525"
     CDS             16626..17633
                     /locus_tag="ACG8KR_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HFQ0199927.1"
                     /translation="MGKVVTLGEIMLRLSTEQGTRIDSSERFAAHYGGGEANVAISLA
                     NYGHEAIFATKVPDNALGKAVKKHLQCYGVDTKQVLSGGSRLGTYYMETGSGERAASV
                     IYDRAGSSFAEMDHLEWSLEELFQGIDIFHVSGITPALSKKWQKMTKQVIEAAKKAGC
                     LISFDINYRGKLWTQQEASEVIHQLLPLVDICSAGKMDALYLLGIEKAPEAEEQPLIY
                     YYQKMQEKFPNIQVFYSTKRTVHSASENELKGTLWMNHEYVESQVHRITPIVDRVGGG
                     DAFAGGVLHGILSNMSPQEIIDFATAASALKHTIHGDCNQFSQEEVAQFLACGSGKII
                     R"
     gene            17649..18491
                     /gene="kduI"
                     /locus_tag="ACG8KR_000526"
     CDS             17649..18491
                     /gene="kduI"
                     /locus_tag="ACG8KR_000526"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008697 -
                     4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0045490 - pectin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HFQ0199928.1"
                     /translation="MQEMETRYTHSPEDIRHYSTEQLRKEFLVEKVFEPGKISLTYTH
                     NDRMIFGGVTPTEKELEITLDKELGVDYFLERRELGVINIGGPGFIEIDGQKEEMKKQ
                     DGYYIGKETRHVVFSSQDAENPAKFYISSVPAHHKYPNVKISIDQVKPMETGEGLTLN
                     ERKIYQYIHPNVCESCQLQMGYTILEPGSAWNTMPCHTHERRMEAYVYFDYANEDTRV
                     FHMMGKPDETKHLVVDNEQAIISPSWSIHSGVGTSNYSFIWAMCGENITYTDMDMVQM
                     DQLK"
     gene            18511..19314
                     /locus_tag="ACG8KR_000527"
     CDS             18511..19314
                     /locus_tag="ACG8KR_000527"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HFQ0199929.1"
                     /translation="MEFSMDMFRLDGKVALVTGAVYGIGFEIARSLATAGAKIVFNNL
                     NQESVDEGIAHYKEAGIDAKGYVCDVTDEEAVQAMVQQIKEDVGSVDILVNNAGIIKR
                     TPMIEMDAADFRQVIDVDLNAPFIVSKAVIPDMIEKGGGKIINICSMMSELGRETVSA
                     YAAAKGGLKMLTKNIASEYGQYNIQCNGIGPGYIATPQTAPLRETQENGERHPFDQFI
                     VGRTPAARWGEPIDLAGPSVFLASSASDFVNGHVLYVDGGILAYIGKQP"
     gene            complement(19563..20627)
                     /gene="fni"
                     /locus_tag="ACG8KR_000528"
     CDS             complement(19563..20627)
                     /gene="fni"
                     /locus_tag="ACG8KR_000528"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HFQ0199930.1"
                     /translation="MNRKDEHVSLAKAFHDKQKNEFDFVRIIHNPLPQIAVSDVDLST
                     QAVGFTLSSPFYINAMTGGSEKTKKINQDLAIVAREADLMIATGSVSAALKVPSLADT
                     YTIMRQEYPHGKIIANIGAGTSVERAKEAIRLFHADALQIHLNAPQELVMPEGDRDFT
                     NWKVLIQETQTAIDVPLIVKEVGFGMTRETLNDLAALGVHTVDISGRSGTSFTQIENA
                     RRSKRELSYLADWGQSTVSSLLEANEADTSMEILASGGIRNAYDIFKALCLGANAVGT
                     SGTVLTHLMNHGVEETIILMKQWQEELRLLYTMVGATNTAALHQQSLIFSGPVKDWCE
                     ARGIDLVKYGRRTEKSVGQK"
     gene            complement(20624..21709)
                     /locus_tag="ACG8KR_000529"
     CDS             complement(20624..21709)
                     /locus_tag="ACG8KR_000529"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004631 - phosphomevalonate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0016126 - sterol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HFQ0199931.1"
                     /translation="MIEVSAPGKLYIAGEYAVVETGHPAVIAAVDQFVTVTVESARKV
                     GSIQSAQYSGMPVRWTRRNGELVLDIRENPFHYILAAIRLTEKYAQEKNILLSFYDLK
                     VTSELDSSNGRKYGLGSSGAVTVATVKALNVFYALNLSQLEIFKIAALANLAVQDNGS
                     CGDIAASCYGGWIAFSTFDHPWLQEQETQHSISELLALDWPGLSIEPLIAPEDLRLLI
                     GWTGSPASTSDLVDQVHRSREDKMVAYQLFLKNSTECVNEMIKGFKENNVTLIQQMIR
                     KNRQLLHDLSAITGVVIETPALNKLCNLAEQYEGAAKSSGAGGGDCGIVIVDQKSGIL
                     PLMSAWEKAEITPLPLHVYSDQRKENR"
     gene            complement(21722..22699)
                     /gene="mvaD"
                     /locus_tag="ACG8KR_000530"
     CDS             complement(21722..22699)
                     /gene="mvaD"
                     /locus_tag="ACG8KR_000530"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0004163 - diphosphomevalonate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HFQ0199932.1"
                     /translation="MFKGKARAYTNIALIKYWGKKNEELILPMNNSLSLTLDAFYTET
                     EVIFSDSYMVDEFYLDGTLQDEKATKKVSQFLDLFRKEAGLSLKASVISQNFVPTAAG
                     LASSASGLAALAGACNTALKLGLDDLSLSRFARRGSGSACRSIFGGFVEWEKGHDDLS
                     SYAKPVPSDSFEDDLAMVFVLINDQKKEVSSRNGMRRTVETSSFYQGWLDSVEGDLYQ
                     LKQAIKTKDFQLLGETMERNGLKMHGTTLAAQPPFTYWSPDSLKAMDAVRQLRKQGIP
                     CYFTMDAGPNVKVLVENSHLSEVQETFTKLFSKEQVITAHAGPGIAIIE"
     gene            complement(22692..23636)
                     /gene="mvk"
                     /locus_tag="ACG8KR_000531"
     CDS             complement(22692..23636)
                     /gene="mvk"
                     /locus_tag="ACG8KR_000531"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004496 - mevalonate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016126 - sterol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HFQ0199933.1"
                     /translation="MANYGQGESSGKIILMGEHAVVYGEPAIAFPFYATKVTAFLEEL
                     DAMDDQLVSSYYSGNLAEAPHALKNIKKLFIHLKKQHDIQKNLQLTIESTIPAERGMG
                     SSAAVATAVTRAFYDYLAFPLSREILLENVQLSEKIAHGNPSGIDAAATSSLQPIYFT
                     KGHPFDYFSLNIDAFLIVADTGIKGQTREAVKDVAHLFETQPHETGQMIQKLGYLTKQ
                     AKQAIIENSPETLAQTMDESQSLLEKLTISNDFLNLLIQTAKDTGALGAKLTGGGRGG
                     CMIALAQTKTKAQEISQALEDAGAAETWIQGLGVHTYV"
     gene            23952..24611
                     /locus_tag="ACG8KR_000532"
     CDS             23952..24611
                     /locus_tag="ACG8KR_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HFQ0199934.1"
                     /translation="MSVIVLAGTIGAGKSSLTEMMAEHFDSQAFYESIDDNEVLPLFY
                     ANPEQYAFLLQIYFLNKRFASIKQAMKDDNNVLDRSIYEDSLLFHLNADLGRATETEV
                     RVYDELLENMMEELPYAAHKKHPDLLVHIRVSFDTMLERIEKRGRSYEQLSFDPSLYD
                     YYKELNRRYDQWYEEYKESPKIQIDGDRYNFVEDPQAKEEVLKMIEEKLAEIRQTKVA
                     I"
     gene            24696..25601
                     /locus_tag="ACG8KR_000533"
     CDS             24696..25601
                     /locus_tag="ACG8KR_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HFQ0199935.1"
                     /translation="MREKHYFNQDRNFWINVQQNDVLAVKNLKEEYGISDEMLTYSLD
                     KNERARVEYDSFDEALLLVSNVPHQQKVDNHYETSPIAFILKEDGLFTFTTPNTEYVI
                     RLIRSLLERMPDMSVYSLLFRTLFLISDSFFPLIEEVNSERQRLNLKLREKTTNKNLL
                     QLSDLEIGLVYLVTGTKQNVVLLEQIKALAIYRKLSEKEKEELDDALIEARQAVEMTN
                     LASQILDQLSGTYNNLLNNNLNDTMKFLTVWSLILTVPTIVTGFFGMNLQLPFTHSVF
                     GWGIALIISLVLSIWMLIALWRRIR"
     gene            complement(25603..26235)
                     /locus_tag="ACG8KR_000534"
     CDS             complement(25603..26235)
                     /locus_tag="ACG8KR_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFQ0199936.1"
                     /translation="MKKIEPIPPSLPLLTKGEFYLEDEEIFSGLLINGGDQSYLDAKN
                     MVLRESHIQKLTMQKTVLRHFECSNVIFEKCDFSNLEWIGGSFHQVVFHQCKLTGTNF
                     AESYLRDCTFTDCIASMASFSSTNLKAVSFDHCQLEDSEFYEVTWKNLFLSDNQLTGS
                     NWFRTSLKGLDFTTNFFTAIALSQDSLAGLIVNQEQALVIAQALGLQIKD"
     misc_feature    26386..26511
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            26555..27079
                     /gene="infC"
                     /locus_tag="ACG8KR_000535"
     CDS             26555..27079
                     /gene="infC"
                     /locus_tag="ACG8KR_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HFQ0199937.1"
                     /translation="MTIAKDMMVNDGIRARELRLIGSDGEQLGVKTKAEALNIAEQAN
                     LDLVLVAPGAKPPVARIMDYGKYRFEQQKKDREARKKQKVINVKEVRLSPTIDLNDFN
                     TKLRNARKFLEKGDKVKASIRFKGRAITHKEIGQKVLNRLAEETADIATVEQKAKMDG
                     RSMFLTLAPKNDSK"
     gene            27151..27351
                     /gene="rpmI"
                     /locus_tag="ACG8KR_000536"
     CDS             27151..27351
                     /gene="rpmI"
                     /locus_tag="ACG8KR_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HFQ0199938.1"
                     /translation="MPKQKTHRGSAKRFKRTGKGGLKRFRAFTSHRFHGKTKKQRRQL
                     RKASMVSSGDFKRIRQQLAKMK"
     gene            27404..27763
                     /gene="rplT"
                     /locus_tag="ACG8KR_000537"
     CDS             27404..27763
                     /gene="rplT"
                     /locus_tag="ACG8KR_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HFQ0199939.1"
                     /translation="MARVKGGTVTRKRRKRVLKLAKGYYGSKHTLFKTAKEQVMNSYN
                     YAYRDRRQKKRDFRKLWIARINAAARMNGLSYSKLMHGLKVAEIDINRKMLADLAVND
                     AAAFTALADQAKEALAK"
     gene            28015..29352
                     /locus_tag="ACG8KR_000538"
     CDS             28015..29352
                     /locus_tag="ACG8KR_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HFQ0199940.1"
                     /translation="MSDIIIGILLVISFFFMVWYCVKGYNLMVGFAIMATVWMGLALV
                     GNTFSPNPAMEGQGVIDVLTHIYTTGPAEYAKSILVNVFFGAFFGRVLVDSGIAATLI
                     RKVVELGGDKPRITMSLLCIVTAVIFMSMTGIGPVISIAVIVLPILMSLGISVPVALF
                     SFMGSIMAGIFANIVNFKQYQTIYAGFNPAAESYTYNDYFQIGMIGMIVSLVVVLTVA
                     NISMNKKKRYAMAANVPAEGGDAPMISWLAVLLPVLGVVLLDLPIILGFILAGIWALL
                     FTGKLRGGYKEICRQFAKLFTDGAVDVAPMVGFLMTLAMFNNSAAYASPYFSAIFGDW
                     IPQSPLVLAIVFAILTPLGFFRGPMNLVGSGSAILAVVLAVNPTMSPAFLFPLFAITT
                     IAPQHLDITQSWVAWGLGYTKVTSREYMKKSIPTGWIIGAILCLITFLLYGNA"
     gene            29400..31529
                     /locus_tag="ACG8KR_000539"
     CDS             29400..31529
                     /locus_tag="ACG8KR_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HFQ0199941.1"
                     /translation="MKRFVRMGIDVGGTHTKAVAIDNATHEIIGKSSVKTTHDDVRGV
                     AAGVVQSFQNCLLENNISPEDVVFVAHSTTQATNALIEGDVAKVGVIGMAKGGLEGFL
                     AKRQTRLNDIDLGNKKKIEIVNAFLPVKHLNVDRVSETISSLERERAEVLVSSMAFGV
                     DNGEPERVVYEAASVKSIPTTMASDITKLYGLTRRTRTAAINASILPKMLDTATSTED
                     SVREAGVNVSLMIMRGDGGVMEINEMKKRPVLTMLSGPAASVMGSLMYLRASNGVYFE
                     VGGTTTNIGVIKNGRPAIDYSIVGGHPTYISSLDVRVLGVAGGSMVRANQSGIIDVGP
                     RSAHIAGLDYAVFTETEKIKGPKVEFFSPKEGDPADYVKVVMEDGEEVTITNTCAANV
                     LGLVQEEHFSYGNVPSARKAIQALADYCHTTVEDIAEQIMEKSYAKIEPVILELADKY
                     HLEKDQISLVGVGGGAASLITYFSNKMGVKYSIPENAEVISSIGVALAMVRDVVERII
                     PSPSKEDIRSLKNEAMNKAIESGATPESIEVHVEIDPQTSKVTAIATGSTEVKATDLT
                     KEITTEEALELAAEDMRLNKNEVCLLENTPFFYVCGEQNRSKNAGSLRIIDQKGFIKV
                     QRGHASCMKTTAANYMTAVEQLWEDMAVYQTELIARPEFYLCLGARVSDFTATDLEQL
                     QLLMDLEVSTMEPEEEVIVVAGNIKQT"
     gene            31504..32193
                     /locus_tag="ACG8KR_000540"
     CDS             31504..32193
                     /locus_tag="ACG8KR_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0199942.1"
                     /translation="MPEISNKHKLEELLNELQQIPEEIWGFYQFRRDLFWKKIPLSKQ
                     KILIQQSIDCGIETACSIKKKYPFADVGEICEQMAVPIVSCESEQINERITFATYAED
                     EGIRLMTEPLEKLKCSGLASISKETAQALIIGHELFHHIEASVKGIYTQSEKIVLWRL
                     PFYTHQSNIRALSEIAAMSFSKEMNQSDFSPYVLEAVLLWPYNEIRSQGILEEIKKIE
                     KRCTEYDLDYK"
     gene            complement(32601..>33264)
                     /locus_tag="ACG8KR_000541"
     CDS             complement(32601..>33264)
                     /locus_tag="ACG8KR_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFQ0199943.1"
                     /translation="QAYESIRKQIIYSELAPGSKISDKVLEEQLAIGRTPIREALIQL
                     RNQELVKTIPQSGTYVSKIDIRSASLARYTREKLEKPILQECSAKMTKQDQAKLEKIL
                     EQTDQAVIAQNKKAFFDLDTDFHRTCYEIAGKREIWDWLESYSTHLNRFRWLRLTISE
                     LDWGRVLDEHQTMLQSMIDHNFDEVGFLCTMHLHMIIEEQEYVIHNYPDYFEDIQDRP
                     IK"
BASE COUNT        10502 a         6017 c         7298 g         9447 t
ORIGIN      
        1 tatacatgtg aattatttaa caaataacca tgacccatta ggaggataat gatggctaaa
       61 gtaatagaaa aagaaaaaac gaagacaatt gacgtacaag caatgattga tgatttggca
      121 gaaaaagcaa atgcagcatt gaaagaaatg gaaagctttg atcaagaaaa agtagaccat
      181 atcgtgcatg aaatggcaat ggcagctttg gatcaacata tgccattagc gaagatggct
      241 gtagaggaaa caggccgcgg tgtttacgaa gataaagcaa tcaaaaatat gtatgcgtcc
      301 gaatatattt ggcataatat caaacatgat aaaacagttg gtgtgatcaa tgaagatgtc
      361 caaaaagggt tgattgaaat tgctgaacca gtcggtgtgg tttgcggagt aactccaaca
      421 acgaatccga cttccacaac gattttcaaa tcaatgatcg cgttgaaaac aagaaatcca
      481 atcgtttttg ctttccatcc aagtgcccaa aaatcttctg cagaagcagc acgagtggta
      541 cgtgatgctg ctatcgctgc cggagctcct gaaaattgta tccaatggat cgaacatcct
      601 tcgatcgaag cgacttctat gctaatgaac catccaggta tcgcgatcgt cttagcgaca
      661 ggtggagctg gaatggtcaa gtctgcttac tctacaggaa aaccagcttt aggtgtaggt
      721 ccaggtaacg taccagctta tattgaaaaa acagcaaaaa ttaaacgtgc agtgaacgat
      781 ttgatcgttt cgaaaacatt tgataatgga atgatttgtg cttctgaaca agctgtgatc
      841 gtggataaag aaatctatgc agcagtaaaa gcagaattcc aagcacacca agtttatatc
      901 gtaaaaccag atgaattgca aaaattagaa gatgcagtga tgaacgaagg aaaatatgcg
      961 gtcaatccat caatcgttgg acattctgcg atggaaatcg caaaattggc aggaatcagt
     1021 gtaccaaaag gaacgaaaat gctgattgct gaattggaag gcgtaggccc agattatccg
     1081 ctatcaagag aaaaactttc tccagttctt gccatgatca aagctaacaa cacggaccat
     1141 gcgtttgatt tgtgtgaagg tatgttgaat cttggaggat taggacatac agcagttatc
     1201 cattcagaaa acgaagagtt gcatgtcaaa ttcggtcttc gaatgaaagc ttgtcgtatt
     1261 ttagtgaata caccatctgc agaaggcggc atcggtgata tctataacga aatgattccg
     1321 tcattgactt tgggatgcgg ttcttatggt aagaactctg tttctcgtaa cgtctctgct
     1381 gtaaacttaa tcaatgtcaa aactgtagcg aaacggagaa ataacatgca atggttcaaa
     1441 ttaccgccaa aaatcttctt tgaaaagaac tctctattgt acttagagaa aatggaaaac
     1501 gttgaacgtg tcatgatcgt ttgtgaccct ggtatggtcc aattcggtta ctgcgataca
     1561 gttcgtgaag tattggcacg acgtaaaaat gatgtgaaga tcgaagtctt ctcagatgtt
     1621 gaaccgaacc catcgacaaa taccgtatac gcaggaacga aactgatggc agacttcaag
     1681 ccagatacag taatcgctct tggaggcggt tctgcaatgg acgcagctaa gggaatgtgg
     1741 atgttctatg aacacccaga cacatctttc tttggtgcaa aacaaaaatt cttggatatc
     1801 cgtaaacgga catataagat cgacaaacca gaaaaaacac aattcgtatg tatcccgaca
     1861 acatcaggga ctggttctga agtaacgcca tttgcggtca tcactgacag tgaaacacat
     1921 gtgaaatatc cgttagcaga ttatgcattg acgccagatg tcgcaatcgt cgatcctcaa
     1981 ttcgtcatgt cagttccagc atctgtcaca gcagataccg ggatggatgt gttaacacat
     2041 gcaatcgaat cctatgtttc agtcatggct tcagattata cacgtggatt gagcctacaa
     2101 gcaatcaagt tagtctttga ctatttagaa aaatcagtaa aaacacctga tatggaatca
     2161 cgtgaaaaaa tgcacaacgc ttctacaatg gctggtatgg cgtttgctaa cgcattccta
     2221 ggtatttgtc actcagtcgc acataaaatc ggcggggaat acgggatccc tcatggacgg
     2281 acaaatgcga ttcttttacc gcatattatc cgctataatg caaaagatcc acaaaaacat
     2341 gcgatgttcc ctaaatatga ctatttccgt gcagatacag attacgcaga tatcgcaaaa
     2401 ttcttaggtc tgaaaggcga aacaacagag gaattagtcg aagctttagc aacggctgtt
     2461 tatgaattag gaaaatctgt cggcatcaac atgagtctaa aagctcaagg agtcacacaa
     2521 gaaacactag atacaaccgt agaccgtatg gctgaattag cttacgaaga ccaatgtacg
     2581 acagctaatc cgaaagaacc attgatctct gaattgaaac agatcatcat tgatgcttac
     2641 aacggataag caaaagaaag cgtagaaaaa agataagaaa atagatgaaa aagaacttgg
     2701 aaaacgttgt ttctccaagt tcttttttta atttccaagt aactgaaagt tacttttgtt
     2761 atttaacatt taggtcaatt ttagacagtt tttgttcaaa aagcgatatt tatagtggac
     2821 ggtattactg ctatattaag gatataaaat gaaagcgttt aaaaaggtgg cgtaaagatg
     2881 attattaaag aagagatttt tgtacctaaa gaattagtga aagaaaaaat tgacctgctc
     2941 gttgaaaacc tgacaacgat caaagacgat aatggtgaat ttttgttgga ctttgatgga
     3001 ctgaaagtag atgacaaaag ctggacagtg tggaattggc cacaaggtgt cggtctatat
     3061 ggtatttata aaaattacca gatgacaaaa aatcctcgcg cttatcaagt ggtcaatgaa
     3121 tggtttgagg atcgtatgga agaaggcgct ccgcccaaaa acgtcaatac aatggcaccg
     3181 ctattgacta tggcttactt gtatgaagac acgaaagatt cacgctattt gccttattta
     3241 gaacaatggg cagaatgggt catgaatgac atgcctcgga caaatgaaga cgggttacag
     3301 catgccacat atggaccgga aaataagaat cagttgtggg acgatacatt gatgatgaca
     3361 gtgcttcctt tggcaaaaat cgggaaactg ttgaatcgcc cagaatattt agaagaagct
     3421 cgttaccagt ttatgatcca tatcaaatac ttgatggata aacgaacagg attgtggtat
     3481 cacggctgga cgtttgaagg aaatcataac tatgccgaag cattttgggc tcgtggaaac
     3541 tgctggttga cgattgctat tccagagatc attgaaatct tagaattatc gaaagaagat
     3601 gcattgcgca aattattgat cgaaacactt gaagcacaag tgcgtgcatt aaaaacttat
     3661 cagtctgaaa gcggtttatg gcatacgttg ttggatgatc cttcttctta cgtagaatct
     3721 tctgcaactg caggatttgc ttacggtatt ttgaaagcgg tccataaacg ttatttacca
     3781 caagaatata aagaagtcgc ttatcgtgct atccaaggat tgcttgaaca aatcgatgaa
     3841 aaaggagaag ttcaaaacgt ttcaatcggg acaggaatgg gggacagttt agatttttat
     3901 cggaatatcg gaatcacagc aatgccttat ggacagtcgt taacggtgct ttgtttgaca
     3961 gaattgctcg tctcttattg ttaaaagaaa ggaaacccat atgaaaaaaa catcactgct
     4021 gtttgcagca agttcgctta gcctaggtct gctaggtcta agcggctgcg gaaataattc
     4081 tagtgtggca actgtcgcag atgcagatgt aaatgagata gttaccttgc gctttgctta
     4141 tgcaagcaac agccagccag taattgatgc aatgaatgaa tttgggcgtt tagtaaaaga
     4201 aaaaacagat ggttccgtac agatccagta tttcccagat ggacaactgg gtggagaacg
     4261 tgaactgatc gaattgacac agtcaggagc agtagatttc acaaaagtaa gtgcctcagc
     4321 gttggaaagt ttctctaaag attattcgat tttttcagtt ccttatctat tcaatgatga
     4381 agaacatttc tttaaggtga tggataatga agaaataatg gagccaatct accaatcgac
     4441 caaaaattta ggatttaccg gattgaccta ttatgattct ggtcagcgca gcttttatat
     4501 ggtagacggt ccgatccata cgccggaaga tttgaaggga aagaagatcc gtgtgatgca
     4561 aagcgaaacc gctatcaaaa tggtagagct tttaggcggt tcgcccgtac ctatgggaag
     4621 tgatgaagtg tacacgtctc ttcaatccaa tttgatcaac ggatcagaaa acaatgaatt
     4681 tgtcttgtat acagctggac atggtggtgt ggctaaatac tattcctatg atgagcatac
     4741 acgtgtgcca gatatcatca tcatgaatga tgccatcaaa gaacagctaa ctgatgagca
     4801 ggaaaaagcc attgaagaag cagcgaaaga atcaacggtt tttgaaattg aagcttttgc
     4861 aaaagcaatt gaagaagaaa aagctttagc acaagaagaa catggagtcc aattcaatga
     4921 tgtagataat acaccgtttc gagaggcagt tgccccactt catgatatat tcaaaaacga
     4981 tcctacctac aaagaccttt atcaaaagat ccaagagcaa ggagcgtagc tgtgatgaaa
     5041 atgattttaa acaaggtatt agagatcctg ggagctgttt tgcttgttgg gatggtcgct
     5101 gttgttttat ggcaagtatt ttccagaacg gttttaggaa atccaaatac ggtcacggaa
     5161 gagcttgttc gattcggtct tgtctggttt gccatgttgt cttccgcgta tgttgtggga
     5221 caaaaagcac atttagccgt aacggtttta agtgaaaaat taactggcaa aaaaagtaaa
     5281 attttagaag ttgttgttca aagtttattt ctggtatttg ctgcgatgat catggtgtat
     5341 ggcggatgga atgctgtcac attgacaatg gggcagatct cgccttcttt aagtattcct
     5401 atgggatatg tttatctgtc agtacctgta tcgggggtac tcatcattat ctatagcctg
     5461 atcaatctaa tcgatacatt aagcaacaag ccagcaattc aagaaaaaat ataaaaatgg
     5521 aggaataaaa tggcaataga agccggatta gtgttatttt ttgtatttgt tgtgttgctt
     5581 gtattaggga tgccgattgc tataagtatt gcattctcat cgatgatgac tttattgtta
     5641 gttataccgt ttgatgtttc tgcttttagt tcagctcaaa aaatggtggg aagtatcaat
     5701 agtttttcgc tgatcgctat tccgtttttc gttctctcgg gcattatcat gaataatgga
     5761 gggatagcta agaaactagt ggattttgcg atgttgttcg tcgggcgcgt gccgggggct
     5821 ttggcttata cgaatgtgtt agggaatgct ttatttggtt ccatctctag ctcggccatt
     5881 gccgcttcta ccgcgattgg cggagtgttg atccctcagc aagtggaaga aggatatgac
     5941 cggaagtttg cgacagcggt caatatcgcg tcagctccga caggtatggt cattcctcca
     6001 agtactgctt ttatcatgta ttcacttgtt gctggtggtg cttcgatctc tgctttgttt
     6061 atgggaggtt atctcgtagg cgctttgtgg gcgctaggaa tcatgctagt agcattcatc
     6121 gttgctaaaa agaataaata ccctgttgtc gaaaaaggac agatggctca tgctggaaaa
     6181 ataataaaag aggcgattcc tagccttcta ttgattgtca tcattatcgg tggtatcttg
     6241 acggggatat ttacagcagt agaagcttca gcgatagctg tcatttactc cctgttgatt
     6301 gctatgtttt actataaaac ggtaaaacta aaagatatac ctaatatgtt gtttcaatca
     6361 gtagcaatgt cagggacgat catgttctta ttggctactt cttccatgat gtctttcgcc
     6421 atggcgttta ccggtattcc tcaggcaatt agttcgttga ttatgggcgt aacggataat
     6481 aaatttatta ttctgttatt agtcaatctt gtgttattac tggtgggtat gtttatggat
     6541 gtggcgcctg ctatcttgat ttttacacca atatttttac caatcgtcac aagtgtaggg
     6601 gttgatcctg tacattacgg gttgttttca atcatgaatc tgtgtgtagg ctccatcacg
     6661 ccgccagtag gtacaggact atatgtcggt gcaagtgtag gaggcgtaaa agcagaggca
     6721 atgctgaaac cgcttcttcc tttttatggg gttatcttag tcgtcttgtt tttgattact
     6781 tacttcccgc aaatcgtcat gtggctaccc aatatgctga gttaggagaa tagttatgtt
     6841 gaacattaca attagaggac atgatctttc cgaagtccaa acgatagaag atcttgcaga
     6901 aaaaacaaaa gaacaaggga ttcatacact gcagctcgcc ttaggtcttt cgtttcctga
     6961 aataccttct ggcgcaaatg agatcaattc tgggatggga aattatgtca aacgagtttt
     7021 agaaaagcaa gaagtgaccg taggaatttt aagttgctat atcaatatga ttcatccaga
     7081 tttaacgata cgacaagagt tgctgacaaa gttcgagcag tatcttcgct atgcttcttc
     7141 ctttggtgcg tcgatggttg cttcggaaac tggatgtgtt ttgccggaaa tccaatatac
     7201 ggaagaaaat ttcacagatg aagcatttgc tgaagctgtg tccgttatcc gtcgcttggt
     7261 aaaagctgga gaaaaatacc aaatgatggt ggggatagaa ccaggtttga atcatccggt
     7321 gtattcttta gcaagggtag agcagctgat ccaagcagta gattcggact atctaggaat
     7381 cattttagat ccgaccaatt tgatcacaag tagtgattat caagaacaag tccaattggt
     7441 agaagaagct tttgaacgtt ttggtgaaaa gatatgtgcg gttcatttga aagattttcg
     7501 tgtcgaacag gaaaaaatcg tgccggtaaa tttgggagac ggtgtgatcg agtacacaaa
     7561 aatcaaagaa atcatcatga agaatcgtcc gtacctgtat gtggttcttg aagaaacaaa
     7621 agatgatggg attcgttatg gaagaagtct tcttgaataa tgtaaagaag acgttggtta
     7681 actaatattt agaatggagg gggcggggct agaagatgcc acgtctcttc ttttttataa
     7741 gagtatataa aagcagctct ttttgattac aaaaaacgtg gtataataaa attgtgcata
     7801 agtgaacaaa aggaggtgcg gcacatgctc caaaagaaaa tcagcggtga acaagtcaat
     7861 acgatggttt ctgatcagta tgaaatctat catgtcaaag atgtcgtcag ccaaaataaa
     7921 acgatctatc atcatcatga tttctatgag atccatgcta cattaaaggg agaagcgact
     7981 ttttatttga acggacgaca atttacgatc aaagcaggaa gtgtgctgct catccattcc
     8041 aatgatttgc atcgtatcgt ccgccaatct tcagatgtat tcgagcgtgt ctatatcttt
     8101 gtcacccctt cttttctaga aagtcgttcc acgccatggt ctaatctatc ggcttgtttt
     8161 caaccaatgg gagagaagag gagtcgtgta ttgcaaatag aacaggaagt attgaagcaa
     8221 aaattatcat ttatcgacca tcctcctgat aaaaaaattt acggtgaaga cataaaatat
     8281 gaacaagctc tggtagaata cttgattttt ctcaatcaag ttgtacaaaa agaagagagt
     8341 gcgcaagaac agaatgctgt agttcctaat gagcgtgtgg aacaaatgtt acaatatatc
     8401 tcaaaaaatt tatctcatcc gctttcttta gaggaaatgg aaaagcagtt ttttgtcagc
     8461 aaataccata tcacacgaga atttaaaaag tacactggat ttacgtttca tcagtatgta
     8521 atgaagaaaa agctgatcta tgccaagcac ttgttgcgag aataccgcag ctctagtgct
     8581 gtatatagta aaagtggttt ttcttcttat ccgcattttc tgaaagcatt caagagagag
     8641 ttcggcataa cgcctaaaga atttttgaaa cgtgatacac agaaacagtt ggttcattat
     8701 gaacattttg aagatgaaca atgagaatga tcactaaaaa ataaccgcag tagctgctgt
     8761 tgtttacata gaagcagaaa ctgcggttat tttattttta tctatatatt tcaaaatatt
     8821 ttgaaaaaat gacgtacata ttatatgaat cagctgaatt tacgaaagtc tctcggcgat
     8881 agtcctgttt ttgctttgaa caggcggcta aagtaagcaa aattctcaat ccctatttct
     8941 tcggaaatca ttcgaaccgt aaattctgta taccgaagaa ggtctttagc gtaagaaagt
     9001 tttttgctga tcaaataatc gattggtgga gaaccgataa atttggaaaa atctttgctc
     9061 agctgatatt tattcaataa gaattgttgt tctaattgat caagtgtaat cgtttctttg
     9121 aaatgttcgt ctaagtattg cttcagtttg taaacgtagg cgggaatatc ctcttcttcg
     9181 aaatctagct gatacttttg caaaatcagt tcattcagca actcgtggat agctacagag
     9241 gactggaaat cggtacgggc attcgtgcct tgttgcatgt ggatcaattc tgtgatcaaa
     9301 tggtggatct tgttttctga tggatctcct gttgaatgga aggtgttatg gccgtctttt
     9361 gtgaactcct gataaaatga ttggacgttc tgaccattca aatggatcca atccatttcc
     9421 catggttctt cgctgatcgt ttgataacgc tgatattctt ggcagtcaat aaaaaataag
     9481 tctccagctt ttagctgttg ctgattatca ttatatgtaa gcgaaccttt gccagacaaa
     9541 gtaaacttta tcaagtaaga aggtaaatgt gctcgttcag tatagtaagg gattggtgct
     9601 ttgaagtaac cgatttcttg gatataaaaa aataaattcc tgcttctttg tgtaggcgtc
     9661 aagatcactc tttttgagtc ttccgaccat gagtggttgg ctttttccca gtaatccatg
     9721 taaacactcc caacttgtca agataatgca attatatacc aaaatagtgt gaagcaaaat
     9781 ataagattct gtattatgat agcattgtca ataaatgata acgctttatt aataaagtgc
     9841 taatcaacaa taaagtaaaa gaggtggaga aatgaatcca acatttgttg ccatcgatat
     9901 cggtgcatca agcggacgac taatgaaaag tacgagggat gcaaaaggtt ctttgatctt
     9961 ggaagaagta caccggttca aaaacggctt tcggaacaaa gacggtcacg atcgctggaa
    10021 gatcgattat cttatcaaag agattttgat tggatttgaa aaattaaagc agacagggat
    10081 tgatcgcctt tttgcgggga tcgatacatg ggcagtcgat tactgtctga ttgatgaaca
    10141 aggggatcgg ataaatgatc cgattgctta tcgggacaag cgtaccaatg gagcagtaga
    10201 acagttttct gaaatctatt ctctagataa gttgtatgag cagacaggga ttcagatcca
    10261 gcctttcaat accttgttcc aattgtttgc tgaagaaaaa gaaaatttgg caaaagcaca
    10321 taaattgtta ctgatacctg attatttagg gtatgtcttt actggtaaca tggtgacgga
    10381 gaaaactaat gcatctacta tgcagctgct gaatgcaggg acaaaagaat gggaaataga
    10441 acttcttgaa aaaatcggtg tctcgaagga attatttcca ccattgacag agccgggaac
    10501 gatactagga gatctgcaaa aagaacaatt ttcaaaatat gatttgccag atgtgacctt
    10561 tatcacagta gcaagtcatg atacggcgtc agctgtatta ggaacgccag gtcaaggaga
    10621 acgttggggt tatatcagca gtgggacatg gtcgctttta gggattgaaa caaagatcgc
    10681 caatgtttct cgcaatgctt tttctgaaaa ttatacgaat gaatggggtg cccataacac
    10741 ggtgcgtttt ttgaaaaaca tcatggggat gtggttgatc caggaagttg cccgaatgca
    10801 agagtacgag tacagttatg cggaattggc agagcttgct gcaaaagaaa aaccgctgca
    10861 gcaagtgatg gaaatcaatg accctcgatt tttgaatccc ccaaatatga tagaagagat
    10921 tcagcaatac tgttttgaaa cagagcagcc agtccctgtc tcatcgggag ctatcgctcg
    10981 ttgtgtgtat gataatcttg ctctttgtta cgcacaagag ctgatcaaag tagaacactt
    11041 aactggaacg aaattggaaa agctgcacat cgtagggggc ggatcgaata atcgtctact
    11101 gaatcaattg acagcagatt atgccggaat cattgtagaa gcaggaccta gtgaagccac
    11161 agcaatcggg aatgttgcca tgcaaatgct cagtacaggt cagttttcga atatagaaca
    11221 agcaagaaat gtaatcaaac agtcgtttcc atgtgaaact ttttacccag atgaaaataa
    11281 gcaaaacacg atggaatggt acgtgaataa aatcaaagga gtaatcgtat gaacacgatt
    11341 gaaagaaatt acgaacaagc aaaagaaaaa tatgcaacta tcggtgtaga tacagatgct
    11401 gtactagaaa agatacagga catcaaaatt tctatgcatt gctggcaagg agatgatgtc
    11461 aaaggtttct tgacaccgga tggcgaattg accggtggaa tcatggcgac agggaatttt
    11521 ccaggagccg cacgaacacc tgaagagctt agacaagatc tggagaaggc ttattcgttg
    11581 ataccgggca aacacaaatt gaaccttcat gctatctatt tggatacaga agaatctgtc
    11641 gatctaaatg aaattgagcc gaaacatttt gaaaaatggg tagagtgggc aaaaaaagaa
    11701 gaaatcggtc ttgacttcaa tccgactttc ttttcgcatc caatgatgaa agacggcttt
    11761 acattggctc atccagataa agaagttcgt gaatactgga tcgaacacgg gaaaagaagc
    11821 cgcaaaatcg cggagtactt tgggaaagaa actgggcaaa caagtatcaa taacttttgg
    11881 gtacctgatg ggatgaaaga taatccgatt gatcgtttta ccccacggga acgtttaatg
    11941 gaatcactcg atgaaatttt tacagaagag ttagatgaac ggtatgtaca agatgcagta
    12001 gaaagcaaac tatttggttt aggcgctgaa gcttatacag tcggttccca tgaattttat
    12061 atgggttacg ggttgactcg tgacaaactt gtttgtctag atgctggaca ttttcatcca
    12121 acagaagtga tttccaataa attatcttca ttagctttat tcagcaaagg aatcatgttg
    12181 catgtttccc gtccagtgcg ttgggattca gaccatgtgg ttttgatgga tgatgaattg
    12241 caggaaatcg ctaaagaatt agtccgtaac gaattgttgg aaaaaacaaa tatcggtctg
    12301 gatttctttg atgcaacgat caaccgtatc gctgcttggg tcatcggtac gagaaataca
    12361 caaaaagcac tgctgaaagc tatgctggaa ccagttgagc gattgaaaga aatggagctg
    12421 gcttttgact ttacttcaag aatggcttat acggaagaat tgaaagattt cccgtatgcc
    12481 gatgtatgga attatttctg ttacaaaaat caagttccag ttggtttgga ttggctggaa
    12541 gaagtccagg aatatcaaaa agacgtgttg gaattaagaa aataggagtg tggctatatt
    12601 gaaaaaaata gatattttac aagcgcctta tgtgaaagaa atgatcgaaa cgacgtcgaa
    12661 tctttatcgc ttgggatggg atgaaagaaa tggcgggaat atttcttatt tactaaaaga
    12721 agaagagatc acaccgttct tggatcctga aaaagtgatc cgccgcatct cgatgatttt
    12781 tgatgccagc cgtttagcag gatgctattt tattgtaact ggttcaggaa gatattttaa
    12841 aaatgtagcg agcgaacctg ccgagaatct tggaatcgtt cgagttgctg aagatggcaa
    12901 aacactggaa cttttgtggg ggctggaaaa cgaatgcctt cctacaagcg agttgccaag
    12961 tcatttcatg agtcatatcg cccgtttaga agtagaccca gataatcgga tcgttatgca
    13021 ttgccatgct agtcatctat tagcgatgag ttttacgcat gaattagacg aacgttcctt
    13081 tagtcgtacg ctatggcaaa tgtgtacgga atgcttagtt gtttttccag aaggaatttc
    13141 catcattccg tggatggttc cgggaacaaa tgagattggg gaagcaacag ctgaaaaaat
    13201 gaaagaaact cgattggttt tatggccaca acatggaatc tacgggtcag gaaaagacat
    13261 ggatgaagtc tttggtttga tcgagacagc agaaaaggca gcagaagtct atacgtacgt
    13321 caaagcgcaa ggaccaatct tgcaaacaat cacggacgaa aacctgtggc atttagcaga
    13381 tgcgtttggt gtcactccaa aagcaggtta tttggaggaa gttcatacga aagcaggtgt
    13441 ctgacatggt gaaaaaagct ttttgcatga aagtttaccc agaaaaacaa gaagaataca
    13501 aaaaaagaca tgatgaactt tggccagaaa tgcgtacagc acttcgtgaa cacggggtca
    13561 tcgactattc gatctatctt gatccgagta caagtttgtt atttggttac ttagtgatcg
    13621 aagatgaaaa gttatgggaa aaaatggcaa atacatctat caatcaaaaa tggtggaagt
    13681 acatggaaga tatcatggag acaaatccag atgcctctcc agtcagttgg gaattgatgc
    13741 cagtctttga actgtagcaa caagcaactg ggcaaccggt tgcttggggt tctttcgttt
    13801 ttattgtgaa aaataataca aattaggagg aatagaaaat gagtaatcga atgattttaa
    13861 atgaaacatc gtattttgga aaaggagcga ttgagtcgat cgtccctgaa tttcagcagc
    13921 gaggattcgg caaagcagct gtcattactg acaaaggatt gatcgagcac ggtatagcaa
    13981 caaaagtaac agaacttcta gataaagcag caatcgctta tgctttatac gacgggatcg
    14041 tacctaatcc aacgatccaa aatgtaaaag atggagtggc ttttgttaaa gaggcagagg
    14101 cagactgttt gatcgcaatc ggtggaggtt cgccaatcga tacggcaaaa gccattggga
    14161 ttattttgac gaatccagag ttttcagacg tcattagttt agaaggtgtt gctgatacga
    14221 aaaatccatg tttacctata ttagctatac caacgacttc tggaacagca gcagaagtaa
    14281 cgatcaacta cgtcatcaca gatgaagtaa atcaccgaaa atttgtttgt gttgatcctc
    14341 atgatatccc aatcgtcgcg ttcatcgaca gcgatatgat gatgggaatg ccgaaaaaat
    14401 tggctgcatc aacaggtatg gatgctatga cgcatgctat tgaagggttt atcacaaaag
    14461 gcgcttggga aatgactgat atgctgcatt taaaagcaat cgaaatcatc ggccattctt
    14521 tggaagcttc tgtagatggc gatcaaaatg ggcgagaaaa aatggcatta ggacagtata
    14581 ttgcgggaat gggcttctca aatgtcggtt taggactggt acacgggatg gctcacccgt
    14641 taagtgcatg gtacaacatt cctcatggcg tagcgtgtgc agcacttttg ccaactgtca
    14701 tgaaatataa caaagaatat actggagaaa agtaccgtga gatcgctctt gttctaggga
    14761 ttaaaggagc agctgaaatg tctttagaag atgtaagaga agcggcttgt ggtgagatcg
    14821 atcggttgag caaagcggtt ggaatcccag aaaccatttc tgaattaggt gtaaaagaag
    14881 cagatattcc agcaattgca gaagatgctt tgcgagatgt atgcacacca ggaaatccaa
    14941 gagaaacgac agtagaagaa atcattgctt tatatcaaag tttgatgtaa aaacgaagcg
    15001 gatcttcaac attaaattgc ctgtaaagag aaatggtagt ttatatctgg atatctcttt
    15061 taaattgatg gtatttccgc tataattttt cttataaaga aataggagaa tattatatgg
    15121 aaaataaaaa gccttatgga actgtattga tcaaagcctc taaaatacta gactatttag
    15181 cggaaaacca agatgtctct ttgcagacga tcgcaaaggg tgtgggaatg acctcttcta
    15241 cagtcttgaa aatactagat actttgttga tgatcggcta cgtaaataaa aattcagaga
    15301 aaaactatcg tttaggtgca aagcttattc gctacgcgaa taaaaacatt gaacaaatcg
    15361 atttggtaga gttgacttta ccgtttttag aacagctgca gcaaaagatc gatgagacta
    15421 tccacttagg ggtattggac aacaatgaga tcttgtacgt gaacaaacta gatccaaaac
    15481 atcaaacgat ccgcatgtct tcaaaaatcg gaatcacccg accattatac aattctgcga
    15541 tgggcaaagc cgtattagca gaattcagtg aagagcagta cactgattat cttgaaaagc
    15601 atccgttgat cccttacaca gaatatacga tcaccaatgc attacggttg aaaaaggaaa
    15661 tcaaaacggt aaaagaaacc cacatcgcat ttgacgatga agaaatcgaa cgtgatattt
    15721 tttgtatagg agcctcaatc gtgaaaaacg aacaaatcat tggtgcattt agtgtaagta
    15781 tgccgaaata tcgtttaaca gatgagacaa aagaacaaat aatcagtgcc ctcaagcaaa
    15841 caaaaagcga aatcgagcaa agaattcaaa aataatgaaa aaagcgtttg caatttttct
    15901 tttctgccgt ataatatggg tatagatttc caagttataa aataaaattt ctaaatagga
    15961 aataggtgga gaagatgaaa aaaattgata ttttatctcg attgaaaaac gccggagtga
    16021 ttgcggtggt acgcggaaaa agcaaagaag aagctttgaa tgcttgccac gcaatcatca
    16081 aaggtggtct aactggtatt gagttgactt ttacagtccc tcaagcagat caagtaatca
    16141 aagaactttt gtcattctac aaagaccaac cagaaatagt gattggcgca ggcacagtat
    16201 tagacgctgt cacagctcgc ttggctatct tagctggcgc agagtatatt gttagccctt
    16261 cctttgatca agaaacggca gagatgtgca atctgtatca aattccttac ttaccaggat
    16321 gtatgactat taccgagatc aaaacagcgc tgaaaagcgg ggtagatatc gtcaaactat
    16381 ttcctggaag cgcttatgga ccaagtatca tttctgcctt caaggcgccg atgcctcaag
    16441 tcaatatcat gccaactggc ggtgtgagtc tggacaatat gaaagagtgg ttcgatgctg
    16501 gtgtcgtaac agtcggagta ggtgggaacc ttttagcacc cgcagctaca ggagattttg
    16561 acaaagtaac ggaagtcgcg caacagtatg cagcaaaaat gaaagaaatc aagaggtagc
    16621 tgcttatggg aaaagtagtg acactcggag aaatcatgtt gcgattgtcc acagagcagg
    16681 gaacccgtat cgattcaagt gaacgattcg ctgcccatta tggcggaggg gaagcaaacg
    16741 tagcaatttc tttagctaat tacggacatg aagcgatttt tgctacaaaa gtacctgata
    16801 acgcattggg aaaagcagtc aaaaagcact tgcaatgtta tggagtagat acgaaacagg
    16861 tattatctgg tggatcaaga ctaggtactt attatatgga aactggtagt ggcgaacgcg
    16921 ccgcctcagt gatctatgat cgtgccggat caagttttgc tgaaatggat catttggaat
    16981 ggtcgctaga ggagttattc caaggaatag atatatttca tgtttcagga atcaccccag
    17041 ctttgtctaa aaaatggcaa aaaatgacga aacaggtgat agaagctgca aaaaaagctg
    17101 gctgcctgat cagcttcgat atcaactatc gtggaaaatt atggacacag caagaagcca
    17161 gcgaagtaat ccatcagctt ttacctttag tagatatctg ctcagctgga aaaatggatg
    17221 ctctttattt gttaggtatc gagaaagcgc cagaagcaga agaacaacct ctaatttatt
    17281 attatcaaaa aatgcaggaa aagttcccga atattcaggt gttttattcg accaagcgta
    17341 ccgttcattc agcaagtgaa aatgaattga aggggacatt gtggatgaac catgaatatg
    17401 tggaatccca agtccataga atcaccccaa tcgtggatcg agtaggtggc ggagatgctt
    17461 ttgcaggtgg cgtgctccat gggattttat ccaatatgtc tccgcaagaa atcattgatt
    17521 ttgctacggc agcttctgcc ttgaagcata cgatccatgg ggactgcaac caatttagtc
    17581 aagaagaagt cgcacaattt ttagcatgcg gctctggaaa aatcattcga tagaaggagt
    17641 agaagaaaat gcaagaaatg gaaacacgtt atacacacag tccagaagat atccgtcatt
    17701 attcaacgga acaattgaga aaagaatttt tagtagaaaa ggtatttgaa ccagggaaaa
    17761 tcagtttgac ttatacgcac aacgatcgaa tgatctttgg tggagtgaca cctactgaaa
    17821 aagaattaga aatcacatta gacaaagaac taggtgttga ttacttctta gagcgtcgtg
    17881 aactaggtgt gatcaatatc ggtggaccag gattcatcga gatcgatggg caaaaagaag
    17941 aaatgaaaaa acaagacgga tactatatcg gaaaagaaac acgacatgtt gtattttctt
    18001 ctcaagatgc tgaaaaccca gctaaattct atattagttc tgttccagct catcataaat
    18061 atccaaatgt aaaaatcagt atcgaccaag taaaaccaat ggagactggt gaaggcctga
    18121 cactgaatga acgtaaaatc taccaataca tccatccaaa tgtatgcgaa agctgtcaat
    18181 tacaaatggg ttatacgatt ttagaaccag gaagcgcttg gaatacgatg ccttgtcata
    18241 cacatgaacg tcgtatggaa gcttatgtct actttgatta tgccaatgaa gatacacgtg
    18301 tgttccacat gatgggcaaa ccagatgaaa caaaacattt agttgtagac aatgaacaag
    18361 ccattatttc gccaagctgg tcgatccatt caggtgtagg tacaagcaat tactcattca
    18421 tctgggcaat gtgcggagaa aatatcacct atacagacat ggatatggtg caaatggatc
    18481 aattaaaata agcataaggg agagaaaatg atggaattta gtatggatat gtttcgttta
    18541 gatggaaaag tagcattagt caccggtgct gtctatggta ttggatttga gattgcacgc
    18601 tctttagcta cagcaggagc aaaaatcgta tttaataact tgaatcaaga atcagttgat
    18661 gaaggtatag cgcattataa agaagcaggc atcgatgcaa aaggttatgt ctgcgatgtg
    18721 accgatgaag aagcggttca agcgatggtt caacagatca aagaagatgt aggttcagtt
    18781 gatattctag tcaacaatgc agggatcatc aaacggacac cgatgatcga aatggatgcg
    18841 gcagacttcc gccaagtaat cgacgtggat ttaaatgccc cattcatcgt ttcaaaagca
    18901 gttattccgg atatgatcga aaaaggcggc ggtaaaatca tcaatatctg ctccatgatg
    18961 agtgaattag gacgcgaaac ggtcagtgct tatgctgcgg caaaaggcgg attaaaaatg
    19021 ctgacgaaaa acatcgcttc tgaatacggt caatacaata tccaatgtaa tggaattgga
    19081 ccaggttata ttgctacacc gcaaactgct ccattacgcg aaacacaaga aaacggtgaa
    19141 cgtcatccat tcgatcaatt tatcgttgga cgtacgccag cagcacgctg gggcgagcca
    19201 atcgatttgg ctggaccatc tgtcttcttg gcttctagcg catctgattt cgttaacggc
    19261 catgtcttgt atgtggacgg aggcatactt gcctatatcg gaaaacaacc ttaaaagagc
    19321 tttagtacac tgtttagtgg agaggttgtg aaatcagcgg attgaacgaa taccgaagga
    19381 cagctgatta aacatcattt attcgaaaaa agaggctggg acagaagcat ttaactccaa
    19441 gaaataagaa ggaattcacg aaaattgctt ttcaaatttt tgtgaatttc agcttatttc
    19501 cgaaggagtt gcttttactt ccgccgtttg tacgtgttta gagcgtgaga caaaagtgtt
    19561 ctttactttt gtcccacgct cttttctgtg cgtcttccat acttcactaa gtcgatccct
    19621 cttgcttcgc accaatcctt cactggtccg gaaaagatca gagattgttg gtgtagggcc
    19681 gcggtattcg ttgcgccgac catagtataa agcagacgaa gctcttcttg ccattgcttc
    19741 atcagtataa tagtttcttc tacgccgtga ttcatcagat gcgtcagtac ggttcctgaa
    19801 gtacccacgg catttgcgcc gagacaaagg gctttaaata tgtcataggc attgcggata
    19861 cctccggagg ctaggatttc catagaagtg tctgcttcat ttgcttcaag caatgaagag
    19921 actgtcgatt gcccccagtc agctaaataa ctcagttccc gcttggagcg acgggcgttt
    19981 tcgatctggg tgaagcttgt tccgcttcga ccgctgatat caactgtatg aactcctaaa
    20041 gcagctagat cattgagcgt ttctcttgtc atgccaaaac cgacttcttt tacgatgagg
    20101 gggacgtcaa tggctgtttg agtttcttga ataagtactt tccaattggt aaaatcacgg
    20161 tctccctcag gcataaccaa ctcttgaggt gcattcaaat gaatctgtaa ggcatctgcg
    20221 tgaaacaatc ggatagcttc tttggctctt tcgacagaag tccctgcacc gatgttggca
    20281 atgattttcc cgtgaggata ttcttgtcgc ataatcgtat aagtatcggc taaagaaggg
    20341 actttcaagg cagcgctgac tgaaccagtg gcaatcatca aatctgcttc tcttgcgacg
    20401 attgcaagat cttggttgat tttttttgtt ttttcgcttc cgccagtcat cgcattgata
    20461 taaaatgggc tgcttagtgt aaacccaact gcctgtgtac ttagatcgac atcagaaact
    20521 gctatttgcg gcaaagggtt atggatgatc cgtacgaagt caaattcgtt tttttgttta
    20581 tcatgaaagg ctttagctag tgaaacatgt tcgtcttttc gattcatcgg ttttcctttc
    20641 tttgatcgct atagacatgt aacggcagtg gagtgatttc tgctttttcc catgcactca
    20701 ttaaaggaag aatgccagat ttctggtcaa caattacgat tccgcaatcg cccccacctg
    20761 caccagaaga ttttgcggct ccttcatact gttcagctaa attacacaat ttgttcaaag
    20821 caggcgtttc gatgacgacc ccagtgattg cagataaatc atgcagtaat tgtcggtttt
    20881 ttcgaatcat ctgttggatc aacgttacat tattttcttt aaaccctttg atcatttcgt
    20941 tgacacattc tgtactattt tttaagaaaa gctgataagc caccatttta tcttctctcg
    21001 aacggtgaac ttgatcgacc aaatcagaag tagaggcagg gctacccgtc caaccaatca
    21061 ataaacgtaa atcttcagga gcaatcaatg gctcaatgga tagacctggc caatccaggg
    21121 caagtaactc actgatagaa tgctgagttt cttgttcttg gagccaagga tggtcgaagg
    21181 ttgagaaagc gatccagcca ccataacagc tagcagcgat gtcgccgcag gaaccattat
    21241 cttgaactgc taaattggct agtgctgcaa tcttgaaaat ctccaactga gataaattca
    21301 acgcgtaaaa aacattcaag gctttgaccg ttgcaacggt cacggcaccg ctagaaccca
    21361 atccatattt ccggccattt gaactgtcta actcacttgt tactttcaga tcataaaagg
    21421 ataaaagaat gtttttttct tgtgcatact tttcagtcaa gcgaatagca gcaaggatat
    21481 aatgaaaagg attttcccga atatctaaaa ctaattctcc gttgcgtctt gtccaacgta
    21541 caggcatccc actatattgt gcagattgga tacttccgac ttttcgtgca gattctacag
    21601 tgactgtcac gaattgatcg actgcagcga taactgctgg atggcctgtt tcaacaactg
    21661 catattctcc ggcaatatag agtttgcctg gtgcagatac ttcaatcata aaaatctccc
    21721 tttattcaat aatcgcaatt cctggaccag catgtgcagt aattacttgt tctttgctaa
    21781 ataatttggt aaatgtttct tgtacctcag ataaatggct gttttccact aaaactttga
    21841 cattagggcc agcatccatc gtaaagtagc atggaatgcc ttgctttctc aattgtcgca
    21901 ccgcatccat cgcttttaag ctgtcaggag accagtaagt gaatggaggc tgggcagcta
    21961 aagtcgttcc atgcattttt agtccgtttc tttccatcgt ttctccgaga agttggaaat
    22021 cttttgtttt gattgcttgt ttcaattgat atagatcccc ttcaacggaa tctaaccagc
    22081 cttgataaaa actggatgtt tcgactgtcc gacgcatccc atttctgctg gacacttctt
    22141 ttttctggtc gttgatcaaa acgaaaacca ttgctaaatc gtcttcgaaa gaatcggaag
    22201 ggactggctt agcgtaagaa cttaagtcgt catggccttt ttcccattcg acgaaaccac
    22261 cgaaaatact tcggcaagct gaaccagacc cgcgtcgagc aaatcttgaa agagagagat
    22321 catctaatcc aagcttaaga gcagtattgc aagcgcctgc taaagcagct agcccgctgg
    22381 cagaggaggc taatccagct gcggtaggaa cgaaattctg actgattact gaagctttta
    22441 gtgagaggcc ggcttcttta cgaaaaaggt caagaaactg actgactttt tttgttgctt
    22501 tttcgtcttg caaggtgcca tctagataaa attcatctac catatagcta tcagaaaaga
    22561 tgacttctgt ctctgtataa aatgcatcca atgttaacga aaggctgttg ttcattggaa
    22621 ggatgagttc ttcatttttc ttaccccaat atttgattag agcaatgttg gtatatgcgc
    22681 gtgctttgcc tttaaacata ggtatgtact cctaatcctt gtatccaagt ttcagcagca
    22741 cccgcatctt caagtgcttg actgatttct tgggcttttg tttttgtttg tgctaatgca
    22801 atcatacagc caccgcgtcc accgccagtt aatttggcac ccaatgcccc ggtatctttt
    22861 gctgtttgga tcaatagatt aagaaaatca ttgctgattg tcagcttttc cagaagtgat
    22921 tgtgattcat ccattgtctg tgccaacgtt tctggtgaat tttcaatgat tgcttgtttt
    22981 gcttgcttcg tcaagtatcc taatttttgg atcatttgtc cagtttcatg aggctgggtt
    23041 tcaaagaggt gagcgacatc tttgacggct tctcttgttt gtcctttgat tcctgtatca
    23101 gcgacaatca aaaaagcatc gatgttcaaa gaaaagtagt cgaaaggatg cccttttgta
    23161 aaataaatcg gctgcaagct gctagtagcg gctgcatcga ttccactagg attaccatgg
    23221 gcgatttttt ccgaaagctg gacattttct aatagtattt cacgagacaa aggaaatgct
    23281 aagtaatcat aaaaagcacg agtgactgct gtggcgactg cagcgcttga tcccattcca
    23341 cgttcagcag gaatcgtgct ttcaatggtc agttgcaagt ttttttggat gtcatgctgt
    23401 ttttttaagt gaatgaataa ttttttgata ttttttaatg catgaggagc ttcggctaaa
    23461 tttcctgaat agtaggaaga aaccagttga tcgtccattg catccagctc ttcaaggaat
    23521 gcggtgactt ttgttgcata gaaaggaaag gcaatcgctg gttctccata aacaaccgcg
    23581 tgctcgccca tcaatatgat ctttccgctt gattctcctt ggccatagtt tgccataatc
    23641 taactccttc tcacgtaaaa gtgattttta agcaaaaaat aaagcgccca aaacgagttg
    23701 cgctgcttca cgaatttttt tgtccactaa gggtctcaaa aaacattcct cctttatttt
    23761 actaaaacca tctcagaagt aaaactaaga atacgttttc taaagaaaaa acactttttt
    23821 cttaagaatt ttttgacttt ttttcttctt tgactagaaa aaaatacaat tgacactact
    23881 agatattggg ggtatattta aaaaggttgg agttaaacac aatatttagt gatgaagtga
    23941 gctggaggta aatgagcgtg atcgttttag caggtacaat tggtgccgga aaatcaagtt
    24001 taacagaaat gatggctgaa cactttgata gtcaggcatt ttatgaatcg attgatgata
    24061 atgaagtctt gcccttattt tatgcaaacc ctgaacagta tgcttttttg ctgcaaattt
    24121 attttttgaa taaacgtttt gcaagcatca aacaagcaat gaaagatgac aataatgtat
    24181 tggaccgttc gatttatgaa gattcactgc ttttccatct aaatgcagat ctaggaagag
    24241 caacagaaac agaagttcgt gtgtatgatg aactgctgga aaacatgatg gaagaacttc
    24301 cctatgctgc ccataaaaaa catccggatt tattagttca tattcgtgta tcttttgata
    24361 cgatgttgga gcgtatcgaa aaacgtggtc gttcgtatga acaattgtcc tttgatccaa
    24421 gtctatacga ttattacaaa gaattgaaca gacgttacga tcaatggtat gaagaataca
    24481 aagaaagtcc aaaaatccaa atcgatggag accgctataa ttttgtggaa gatcctcaag
    24541 caaaagaaga agtcttgaaa atgatcgaag aaaaattagc agagattcgc caaaccaaag
    24601 tagccatttg atttacagtt gctcaaataa agggtaaacc tttatttgag cgactttttt
    24661 ctttatactg gaaacaaatg tcaggaggat agagtatgcg ggaaaaacac tattttaatc
    24721 aagaccgtaa tttttggatc aatgtacagc aaaacgatgt gctagctgtt aaaaacttaa
    24781 aagaagaata tggcattagt gacgagatgc tgacttattc tctagataaa aatgaacggg
    24841 cgcgcgtaga gtacgattct tttgatgaag cgttactgct tgtatcaaat gttccgcacc
    24901 agcaaaaagt cgataatcat tatgaaacta gtcctatcgc ttttatttta aaagaagacg
    24961 gtttgtttac atttaccacg ccgaataccg agtacgtgat tcgactaatt cgatcgcttt
    25021 tggaacgaat gccagacatg agtgtataca gtttattgtt tcgtacattg tttttgattt
    25081 ctgattcctt ttttccttta atcgaggaag tgaatagtga acgtcagcgg cttaatctga
    25141 aattaagaga aaaaacaacg aacaagaatc tgctgcaact ttcagattta gagatcggtt
    25201 tggtttactt agttacagga acaaaacaaa atgtagtact tttagaacaa atcaaggcat
    25261 tggctattta tcgcaagttg tcagaaaagg aaaaagaaga gttggacgat gcgctgatcg
    25321 aagcacgaca agcagttgaa atgacgaact tagcttcgca gattttagac cagctctctg
    25381 gcacttataa taatttgctg aataacaact tgaatgacac gatgaaattt ttgacggtct
    25441 ggtcgctgat cttgaccgtt ccaactattg tgacaggatt cttcggaatg aatctccagt
    25501 taccctttac ccattccgtt ttcggatggg gaatcgcctt gatcatcagt ttggtcttat
    25561 ctatttggat gcttattgca ctgtggcggc ggattcgttg atctagtctt tgatctgcaa
    25621 accaagggct tgggcgatca caagtgcttg ctcttggttg acgattagtc ccgccaaaga
    25681 atcttgagat aaagcgattg ctgtgaagaa attagtcgta aagtctaatc ccttcagaga
    25741 agttcgaaac cagttcgaac cggtcaactg attgtcagac aaaaaaaggt ttttccaagt
    25801 gacctcataa aattcgctgt cttctaattg acaatgatcg aaggagacag ctttcaagtt
    25861 tgtactacta aaggaagcca tcgaagcaat acaatcagtg aaagtacagt ctcgaagata
    25921 actttcggca aaattcgtac ctgttaattt acattgatga aacacgactt ggtggaaact
    25981 gccaccaatc cactccagat tggaaaaatc acatttttca aaaatgacat tactgcattc
    26041 aaaatgacgt aaaactgttt tttgcatcgt aagtttttgg atatgactct ctcttaatac
    26101 catatttttg gcatcgagat agctttggtc acccccgttg atcagaagac cagagaagat
    26161 ctcctcatct tctaaataaa actctccctt tgtaagaaga ggaagagaag gtggaatagg
    26221 ttcgattttt ttcatgtggg ttcacctcgt ttttctttag tatagcaaag aagcgggaat
    26281 cactgctaga aagtctgcta atctaatcat tttcagaaaa ggaagacaaa ctgtatgaaa
    26341 aacattgaca aaaagattag tttttgctat actatgatgg ttgagaaata aaagcagaag
    26401 cacccgcttc tcgccttagt tgataatgtc actgggctag atagttttca ctggttctat
    26461 taggtgaagt acgcgcgggt tctgtaagag aactcgtttt tttattgccg tttattcggt
    26521 aagcttttct ttctctcata atatttggag gtgaatgacc atagcaaagg atatgatggt
    26581 taacgacggc attcgtgcac gagagttacg attgatcggt tcagatggtg agcaattagg
    26641 agttaagaca aaagcagaag cattgaatat tgcagaacaa gcaaacttgg atcttgtgtt
    26701 agttgcccct ggtgcgaaac caccagttgc gcgaatcatg gactacggta aataccgttt
    26761 cgaacaacaa aagaaagacc gcgaagcgcg taaaaaacaa aaagtgatca atgtaaaaga
    26821 agttcgctta agtcctacga tcgacttgaa tgacttcaat acaaaacttc gtaatgcacg
    26881 taagttcttg gaaaagggcg ataaagtgaa agcttctatc cgtttcaaag gccgtgccat
    26941 tacccataaa gaaattggtc aaaaagtctt gaatcgctta gctgaagaaa ctgcggatat
    27001 tgcaacagtg gaacaaaaag cgaaaatgga cggacgcagc atgttcttga cgctggcacc
    27061 gaaaaacgac agtaagtaat ttgaatgact aactggctgt tggacagccg atagtcggat
    27121 aacgaaagat tttgaggagg aaatatagtc atgccaaaac aaaaaacaca ccgcggatca
    27181 gcaaaacgtt tcaaacgtac tggtaaaggc ggattgaaac gcttccgcgc gtttacaagt
    27241 caccgtttcc acggcaaaac aaaaaaacaa cgccgtcaat tgcgtaaagc aagcatggtt
    27301 tctagtggcg acttcaaacg tattcgccaa caactagcaa aaatgaaata agaagtacaa
    27361 ataacagaat ctgaagattt atttttatta ggaggaatta tacatggcac gtgttaaagg
    27421 tggaacagta actcgtaaac gtcgtaaaag agtgctaaaa ttagcaaaag gttactacgg
    27481 ttcaaaacac actttattta aaacagcaaa agaacaagta atgaattcat acaactacgc
    27541 atatcgcgat cgtcgtcaaa agaaacgcga cttccgtaaa ttgtggattg ctcgtatcaa
    27601 cgcagcagct cgtatgaatg gcttgagcta ctcaaaatta atgcacggtt tgaaagttgc
    27661 tgaaatcgac atcaaccgta aaatgttagc tgacttggct gttaacgatg cagcagcatt
    27721 tactgcattg gctgaccaag caaaagaagc tttggctaaa taagctaaac aattataaag
    27781 aggaaagaag ataacgaaaa gttatgttct ttcctctttt tttatttaca atcctccatc
    27841 aaatgaaaca ggacaataac gtataagtct aaaatagcat aattaaggtt ttttaattga
    27901 aaactgaaca tttatggtca ccctttaatg aaagcgctaa ctacataagt tagaatgagt
    27961 ttgactgata tattagtttg cttatcagaa aaacaaaatg gacggaggaa taacatgagt
    28021 gatattatta ttggtatttt gttagttatc tcattctttt tcatggtctg gtactgtgta
    28081 aaaggctaca acttgatggt cggctttgcc atcatggcta ctgtatggat gggattagcg
    28141 ctagtcggaa acacgttttc accaaacccg gcgatggaag ggcaaggagt aattgatgtg
    28201 ttgacgcata tctatacgac ggggccagct gagtacgcca aatcgatctt ggtaaatgtc
    28261 ttctttggtg cctttttcgg gcgtgttcta gtagatagcg gaatcgcagc taccctgatt
    28321 cgaaaggtag ttgaacttgg aggggacaag ccaagaatca cgatgagctt actatgtatt
    28381 gtaaccgctg ttatattcat gtcgatgaca ggaatcggac cagtgatttc tattgcagtt
    28441 attgtgttgc caatcttgat gtcattaggt atttcagtac cggttgcact gttctcattt
    28501 atgggctcga ttatggctgg aatctttgct aatatcgtca atttcaaaca atatcaaacg
    28561 atttatgctg gtttcaatcc agcggctgaa agctatactt ataatgacta tttccaaatt
    28621 ggtatgattg ggatgattgt aagcttagtt gtggtactaa cggtcgctaa tatatcaatg
    28681 aacaaaaaga aacgctatgc aatggcagca aacgtaccag ctgaaggtgg cgacgcacca
    28741 atgatctcat ggttagccgt attattacca gttttagggg tcgttctttt agatcttcca
    28801 atcatcttag gttttattct agcaggtatc tgggcattgc tgtttactgg aaaattgcgc
    28861 ggaggttata aagaaatctg tcgtcagttt gcgaaattat ttacagatgg cgcagtagat
    28921 gttgcgccaa tggttggttt cttgatgacc ttagcgatgt tcaataattc agctgcgtat
    28981 gcttctcctt atttttcagc tatttttgga gattggatac cgcaatcacc attagtcttg
    29041 gcaatcgtat ttgctatatt gacaccacta ggatttttcc gcggtccaat gaatctagtc
    29101 ggttcaggct cagctatttt agctgtcgta ttagctgtta atccaacgat gtcgccagcc
    29161 tttttgttcc cgttatttgc aatcacaacg attgctcctc agcatttaga tattacacaa
    29221 tcttgggttg cttggggatt aggttataca aaagtaactt ctcgagaata tatgaagaaa
    29281 tccatcccaa ctggttggat catcggagcg atcttatgcc tgatcacgtt cttattgtac
    29341 gggaacgcct agtaaacaaa acgtcgataa aaacgattag atgaatggag gaaaagaaaa
    29401 tgaaacgttt tgtgcgaatg ggaattgatg ttggcggtac acatacgaaa gcagtcgcaa
    29461 tcgataatgc gacgcacgaa atcatcggaa aatcttcagt gaaaacaacc cacgatgatg
    29521 tccgtggagt agccgcaggt gtcgttcagt cttttcaaaa ttgtttgctg gaaaataata
    29581 tcagtccaga agatgttgtg tttgttgcac attctaccac acaagcgact aatgcactga
    29641 ttgaaggaga cgtagcaaaa gtcggcgtaa tcgggatggc aaaaggtgga ttagaaggtt
    29701 ttttagcaaa acgacaaact agattgaacg atatcgattt agggaataag aaaaaaatag
    29761 agattgtcaa tgcattctta cctgtcaaac acttaaatgt agaccgtgtt tcagaaacga
    29821 tttcttcttt agaaagagaa agagcggagg tcttggtttc ttctatggca tttggagtag
    29881 ataatgggga accagaacga gttgtttatg aagcagcatc ggtaaaatcg atccctacaa
    29941 cgatggcttc tgacatcaca aaattatatg gattgacaag aagaactcgg acagcagcaa
    30001 tcaatgcatc tattttacca aaaatgctag atacggcaac ctctacagaa gattccgtac
    30061 gagaagcagg ggtcaatgtt tctttgatga ttatgcgcgg agacggcgga gtaatggaaa
    30121 tcaatgaaat gaaaaaacga cctgttttaa ccatgctttc cggtccagca gcatcagtca
    30181 tgggctcttt gatgtacttg agagcgtcta atggtgttta ttttgaagta ggcggaacaa
    30241 caaccaatat cggggtcatt aaaaatggac gtccagcaat cgattattca attgtaggtg
    30301 gccacccaac atatatctct tcattagatg ttcgggttct aggtgtggca ggcggttcga
    30361 tggtacgggc taatcagtca ggaatcatcg atgtcggtcc acgttctgca catattgccg
    30421 gtctagatta tgcagtattt acggagacag aaaaaatcaa aggtcctaag gttgaatttt
    30481 tctctcctaa agaaggagac cctgctgatt acgtcaaggt agtgatggaa gacggagaag
    30541 aagtgactat tacaaataca tgtgcagcca atgtccttgg attagtgcaa gaagaacatt
    30601 tttcttatgg aaatgtccca tctgcacgaa aagccatcca agcattagct gattattgtc
    30661 atacgacggt tgaagacatc gcagaacaga tcatggaaaa atcctatgct aagattgaac
    30721 cggtgatttt agaattggca gacaagtatc acttggagaa agatcagatt tctcttgtag
    30781 gtgtaggggg cggcgctgcc tcattgatta cttactttag taataaaatg ggtgtgaaat
    30841 attcgattcc tgaaaacgcc gaagtgattt cttctattgg tgtagcactt gcaatggtcc
    30901 gagatgttgt agaacgtatc atcccttcgc caagtaaaga agatatacga tcgttgaaaa
    30961 atgaagcgat gaataaagcg attgaatcag gcgctacacc agaatcgatc gaagtccacg
    31021 tagaaatcga tccacagact tcaaaagtaa cggctatcgc tactggttct acggaagtga
    31081 aagcaaccga tctaaccaag gagatcacaa cagaagaagc actagagctt gctgcagagg
    31141 atatgcgatt aaataaaaat gaagtttgct tattggaaaa tacacctttc ttttatgtat
    31201 gcggcgaaca gaatcgttca aaaaatgctg gaagtcttcg gatcatcgat caaaaaggat
    31261 ttatcaaggt ccaacgtgga catgcttcat gtatgaaaac aactgcagcc aattatatga
    31321 cagctgtcga acaactttgg gaagatatgg cagtttatca aactgaattg attgcccgac
    31381 cagaatttta tctctgctta ggagcaagag tctctgattt tacagcgacg gatctggaac
    31441 aactacagct tttgatggat ctggaagtat ctactatgga accggaggaa gaagtgatcg
    31501 tcgttgccgg aaatatcaaa caaacataag ttagaagagt tactaaacga attacaacag
    31561 atccccgaag aaatttgggg attctaccag tttcggcgag atttattttg gaaaaagatt
    31621 cctttgtcca agcagaaaat tcttatacaa caatcaatcg attgtggtat cgaaacggca
    31681 tgtagcatca aaaaaaagta cccttttgcc gatgtaggag aaatttgtga acaaatggct
    31741 gttccaatcg tttcctgtga atcagagcaa ataaacgaac gaatcacttt tgcaacttat
    31801 gcagaagatg aagggattcg attgatgacg gagccgttag aaaaattaaa atgttcagga
    31861 ctagcttcga tctctaaaga aacagcgcaa gctttgatta ttggacatga actatttcac
    31921 cacatcgaag cttctgttaa gggaatttat acgcaaagcg aaaaaattgt gctgtggcgg
    31981 ttgccttttt atacacatca atcaaacatt cgtgcattga gcgagatagc agcgatgagt
    32041 ttttcaaaag agatgaatca aagcgatttt tcgccttatg tattagaggc agtcttactt
    32101 tggccatata atgaaattcg tagccaaggg atcttagaag aaataaaaaa aatagaaaaa
    32161 agatgtacgg agtatgattt agattacaaa taaagagact gtgacaaaag tcgtgtcaca
    32221 gtcctttttt tccgaataaa cgatgttctc ctgtcaactc ctcggtcaca agtcaaaata
    32281 ttcaacaaac atgagaagct gttttttgaa attgttcctt gtgactcgga gatgaacggc
    32341 aggctcacga cctcttggaa aacggtgttc aaaagcttgc atctgcggta ataagtccag
    32401 ccaagttaaa aacataagaa gctattttta cttggctgtt cttattactt gatgcagggc
    32461 agctttttca caacctctca ataaacggtg tgcaatcagc tgaccctcgg tattagctca
    32521 aaaaaaacgg caaaatatga ggaactattt tgccgtttct ttctctaata ctcaggtcaa
    32581 aacgctgatt ccaaaacctc ttatttgatg ggtctgtcct gaatatcttc aaaataatcg
    32641 gggtaattat gaatcacgta ttcttgctcc tcgataatca tatgtaaatg catcgtgcaa
    32701 agaaatccga cttcatcaaa gttatgatca atcattgatt gaagcattgt ctgatgttca
    32761 tctaatactc gaccccagtc taattctgaa atcgtcaaac gtaaccatct gaaacggttc
    32821 aagtgcgtac tataactttc tagccaatcc caaatttctc gttttccagc aatttcataa
    32881 caagttcggt gaaaatcagt atccaaatca aaaaaagctt ttttattttg agcgattact
    32941 gcctgatcgg tttgttctag tattttttct agtttagctt gatcttgctt ggtcattttc
    33001 gcactgcatt cttgcaggat cggtttttcc aacttttccc gcgtataacg ggctaatgaa
    33061 gcagatcgga tatcgatttt tgaaacataa gtgccggact gtggaatggt ttttacaagc
    33121 tcttgattgc gcagttggat cagtgcttct cgaatcggtg tacgcccgat agctagttgt
    33181 tcttctaaca ctttatcgga gattttactt ccaggtgcta actcggaata aatgatttgt
    33241 ttacgtatag attcataagc ttgc
//
LOCUS       DAZKLD010000013        32810 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.013, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000013 DAZKLD010000000
VERSION     DAZKLD010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32810)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32810)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32810
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.013"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(68..970)
                     /locus_tag="ACG8KR_000542"
     CDS             complement(68..970)
                     /locus_tag="ACG8KR_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFQ0199944.1"
                     /translation="MNIQQMKYVVAVANNGSFREAAKKMFVSQPSLSAGIKELETELG
                     ITLFTRTNRGAYLTEEGQDFLNRAEKILVQLDTLETHYLTSEKRDNFSIAAQHYDFLG
                     PLTAQLLEEFKDEIKQFRIMETTTARVIEEVKEQHSEIGIIYMNEHNQAGIRRYLQQG
                     ELSGRSIGKFKTHIFLRQGHPLAEHAEITKEELLSYPQVRFTQDGNNFPYFYEDLIEI
                     PDQETVIYTSDRGTLMNIVLETDAYASGSGIVIGGIREHLRLIPLAEGELNEFYIIYS
                     AKRPLSEIAQRFIKELTRLLDQQS"
     gene            complement(967..1659)
                     /locus_tag="ACG8KR_000543"
     CDS             complement(967..1659)
                     /locus_tag="ACG8KR_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HFQ0199945.1"
                     /translation="MDVEWGYSGDKGPENWASLCDWFARGAEFPLQSPIHLTKGEETK
                     IEGDTLSFHYQKQRFTDKEFKNTIHLVPFDQLSYVEFKKERYYLTDIHFHLPSEHIIN
                     GKQEDIEFHFVHMNSKKENLVVGVMYQLMVQEGWLYNQENGESWNLEKHEHWVNPDVF
                     FPEQKSHFHYIGSLTTPPTSGPIQWFVMDHVGKLNQEFLLPFDGQYARPNNRPIQPLN
                     GREIYYFEEFEQ"
     gene            complement(1702..2343)
                     /locus_tag="ACG8KR_000544"
     CDS             complement(1702..2343)
                     /locus_tag="ACG8KR_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbR-like 5'-deoxynucleotidase"
                     /protein_id="HFQ0199946.1"
                     /translation="MGLNEFILGLNNLETITRAPGFFKYTEHTVAAHSYRVASIAQVL
                     GDIEEANGQPIDWKLLYEKSLNHDYTERFIGDIKTPVKYASKELRGMLQTVEEKMTDE
                     FINQEIPAEFQEVYRRRLFEGKDETVEGELLAISDKVDLLYESFDEIIKNNPEKVYKE
                     MFIEAVTTIKSFEHRPSVQYFFKEIFPELLNQEFYGKEAFLETVSTIVKKEEK"
     gene            complement(2381..2695)
                     /locus_tag="ACG8KR_000545"
     CDS             complement(2381..2695)
                     /locus_tag="ACG8KR_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0199947.1"
                     /translation="MADLSALDEDREIRFVSPESSIGIAKIQKLIARSIFSSGMKRNF
                     YRDQFLIFLKFIFLRLVSRKKRIISSLFSIIYRFSLFFGKGAVSKMVKSGNVANISYL
                     MI"
     gene            complement(2683..3312)
                     /gene="pyrE"
                     /locus_tag="ACG8KR_000546"
     CDS             complement(2683..3312)
                     /gene="pyrE"
                     /locus_tag="ACG8KR_000546"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HFQ0199948.1"
                     /translation="MNVEQSIAKDLLEIEAVFLNPSNPFTWASGIKSPIYCDNRITMS
                     YPKVRKEIAKGLASKIKEAYPEVQVIAGTATAGIPHAAWVAEILDLPMVYIRSKAKDH
                     GKGNQIEGRIFEGQKMVVIEDLISTGGSVLEAAEAAKREGADILGVAAIFTYELPKGK
                     ANFEKAEIPLMTLTNYSVLIEAALEDRYIDEQELTLLKEWKKDPENWQI"
     gene            complement(3319..4035)
                     /gene="pyrF"
                     /locus_tag="ACG8KR_000547"
     CDS             complement(3319..4035)
                     /gene="pyrF"
                     /locus_tag="ACG8KR_000547"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HFQ0199949.1"
                     /translation="MNQLDQRPIIALDFSTRQEVEDFLRFFPKEEKLFVKIGMELFYQ
                     EGPEIVRYLKEAGHNVFLDLKLHDIPNTVEKAMRGLAKLGVDLTCVHAAGGIRMMEAA
                     MRGLEEGTPEGGKRPLLLAITQLTSTSEEEMHADQLIEVSLEKSVIHYASCAKKAGLD
                     GVVSSAWEVEAIKEMAGDEFVCLTPGIRPEGTVAGDQTRVVTPSQAKKIGSTFIVIGR
                     PITQSTNPYKAYQTIQTEWS"
     gene            complement(4032..4964)
                     /locus_tag="ACG8KR_000548"
     CDS             complement(4032..4964)
                     /locus_tag="ACG8KR_000548"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HFQ0199950.1"
                     /translation="MNENPLAVKLPGLELKNPIMPASGCFGFGKEYGKYYDLNQLGSI
                     MVKATTPNARFGNPTPRVAETPSGMLNAIGLQNPGLDVVMHKLLPELEEYNELPIIAN
                     VAGACEEDYVEVCSKIGEAPNVKAIELNISCPNVKHGGIAFGTDSDVAFQLTQAVKKV
                     SSVPVYVKLSPNVTDIVPIAQAIEAGGADGFTMINTLLGMRIDLKTRKPILANQTGGL
                     SGPAIKPVAIRLIHQVASISSLPIIGMGGVQTVDDVLEMFMAGASAVAIGTANFTDPY
                     ICPKLIEELPVRMSELGIESLERLIKEVREERER"
     gene            complement(4961..5737)
                     /locus_tag="ACG8KR_000549"
     CDS             complement(4961..5737)
                     /locus_tag="ACG8KR_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HFQ0199951.1"
                     /translation="MKQEMMEIVQQKQLAPKIFEMTLKGDLVKEMQQPGQFLHILVPR
                     ADLLLRRPISLNHIDHETNTCRIIYRVEGEGTRVFSTLSAGDHLDVMGPLGNGFDLSN
                     LHTGDEVFIIGGGIGVPPLYELSKQLVEKGIKPTHFLGFATHEVVYYEKEFNRLGETR
                     IATDDGTYGMHGNVGNLLLGTKTEPTAVFACGSNGLLKTVEQLFSSHQNVQLSLESRM
                     ACGIGACYACVCHTPEDETGTKSVKVCDEGPIFKIGEVII"
     gene            complement(5791..8976)
                     /gene="carB"
                     /locus_tag="ACG8KR_000550"
     CDS             complement(5791..8976)
                     /gene="carB"
                     /locus_tag="ACG8KR_000550"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HFQ0199952.1"
                     /translation="MPKRTDIKKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYEV
                     VLVNSNPATIMTDKEVADQVYIEPITLEFVSRILRKERPDALLPTLGGQTGLNMAMEL
                     AASGILEELNIELLGTKLSAIDQAEDRDLFKQLMKELNQPIPESEIVNTVEEAVDFAN
                     KIGYPVIVRPAFTLGGTGGGMCENEEELRIVSENGLSLSPATQCLIERSIAGFKEIEY
                     EVMRDSADNAIVVCNMENFDPVGIHTGDSIVFAPSQTLSDHEYQMLRDASLSIIRALK
                     IEGGCNVQLALDPHSFNYYVIEVNPRVSRSSALASKATGYPIAKLAAKIALGLTLDEM
                     KNPVTGTTFAEFEPALDYVVAKIPRWPFDKFENGERLLGTQMKATGEVMAIGRNIEES
                     LLKAVRSLEIGTHHIELPELADITDDDLIQKVVRAQDDRLFYVAEAIRRGYTIEELAD
                     LTKIDLFFLDKLLHIVEIEQQLKQQPLDDKILKTAKQNGFTDKKVAELWEINEEAVRQ
                     MRKENDLIPVYKMVDTCAAEFESKTPYFYSSYEYENESSRSEKPSILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQQAGYEAIIMNSNPETVSTDFSISDKLYFEPLTFEDVMNVIEL
                     EQPIGVIVQFGGQTAINLAEPLTAAGVKILGTTIEDLDRAENRDLFEQALKELDIPQP
                     LGDTATSKEEAVVIASRIGYPVLVRPSYVLGGRAMEIVENQRDLEDYMEHAVKASPEH
                     PVLVDRYLLGGECEVDAICDGETVLIPGIMEHIERAGVHSGDSMAVYPPQTLSESIKK
                     TIEDYTIRLALGLNCVGMMNIQFVIHENEVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKAILGEKLKTLGYENGLYPETKEVHVKAPVFSFTKLQKVDTYLGPEMKSTGEVMGSD
                     RQLDKALYKAFEASGLHLPEYGAVLFTIADETKEEALELAKRFTEIGYSLIGTKHTAA
                     YFEKNGLVVETVAKISEEAAEKNVVDLIRDGKTQVVVNTIDKDRGNASKDGFIIRREA
                     VEHGIPLFTSLDTADAIVRVMESRAFSTNPI"
     gene            complement(8981..10057)
                     /locus_tag="ACG8KR_000551"
     CDS             complement(8981..10057)
                     /locus_tag="ACG8KR_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl phosphate synthase small subunit"
                     /protein_id="HFQ0199953.1"
                     /translation="MERLLILEDGTVFEGKAFGADASVFGEIVFTTSMTGYQETITDQ
                     SFNGQIITFTYPMVGNYGVNRDDYESISPTCKGVVVKEHARVASNWRNQMTLDSFLKQ
                     KGIPGISGIDTRALTRKIREVGTMKASIVDAGDSFEHAFDQLKATVLATNQVQQVSTN
                     KPYPSPGTGRKVIVIDFGLKHSILRELSKRNCNLTVLPYNTDAETILALSPDGVMLTN
                     GPGDPKDVPEAIEMIKKIQGKVPIFGICLGHQLFALANGADTYKMKFGHRGLNHPVRE
                     VATGRIDFTSQNHGYAVDEATIDPEKLIVTHVEVNDGTVEGVKHRDYPAFSVQFHPDA
                     APGPHDALHLFDEFMEMMDAGKEQ"
     gene            complement(10062..11342)
                     /locus_tag="ACG8KR_000552"
     CDS             complement(10062..11342)
                     /locus_tag="ACG8KR_000552"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HFQ0199954.1"
                     /translation="MKTLIKNGQINTRKNMTTPAEIWIEDGRIKAIGTGFSEAEFDEV
                     FDAKGQLITPGLVDVHVHLREPGFTYKETIEAGTRSAARGGFTTVCAMPNLNPVPDTA
                     EKLRQVYDIIRKDAVVKVLQYAPITENLRSEKLVDQEALIEEGAFAFTNDGVGVQTAG
                     TMYLAMKEAAKNNKALVAHTEDESLLFGGVMHAGKKAEELGLPGILSVTESSQIARDL
                     LLAEATGVHYHVCHVSTKESVRVIRDAKKAGIHVTAEVSPHHLILIDEDIPEDFGFWK
                     MNPPLRGREDREALIEGLLDGTIDCIATDHAPHGLEEKSQSFMKSPFGIVGSETAFQL
                     IYTHFVETGRFTLEQVINWLAVKPAEIFGLNAGTLTVGAPADVAVFDITQTCTIDKED
                     FLSKGENTPFIGWKVKGETQMTFVNGKLVWQKGE"
     gene            complement(11361..12287)
                     /locus_tag="ACG8KR_000553"
     CDS             complement(11361..12287)
                     /locus_tag="ACG8KR_000553"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HFQ0199955.1"
                     /translation="MIIKSERISLKHLLTVEALTDQEVMGLIRRGQEFKQGANWTPQK
                     RQYFATNLFFENSTRTHKSFDVAEKKLGLEVIEFEASTSSVQKGETLYDTVLTMSALG
                     VDVAVIRHGDENYYDELIQSKTIQCSIINGGDGSGQHPTQCLLDLMTIYEEFGHFEGL
                     KVAIVGDITHSRVAKSNMQMLKRLGAQVFFSGPREWYDDEFEVYGHYMPLDELLDQVD
                     VMMMLRVQHERHDGKESFSKEGYHQEHGLTVERAKKLQKHAIIMHPAPVNRDVELADS
                     LVEGLQSRIVQQMSNGVFVRMAILEAVLNGKA"
     gene            complement(12300..13583)
                     /locus_tag="ACG8KR_000554"
     CDS             complement(12300..13583)
                     /locus_tag="ACG8KR_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute carrier family 23 protein"
                     /protein_id="HFQ0199956.1"
                     /translation="MSEKKGFHNDDVVLDIHDRPTPGHWVGLSLQHLFTMFGATVLVP
                     ILVGIDPGIALVSSGLGTLVYLFTTKGKIPAYLGSSFAFIAAMQLLMKTDGYPAIAQG
                     AVTTGLVYLIVALIIKKIGSAWLDKILPPIVVGPVIMVIGLGLAANAATNAMYNQGTY
                     DFKYIAVALITLGLTIFFNMWLRGFLGLIPILLGIISGYIVALLFGIVDTKPIVDAAW
                     FAVPNFEIPFVQYEPKLYLGAITTMAPIAFVTMTEHIGHLMVLNKLTKRNFFEEPGLH
                     KTLTGDGLAQIVAAFVGGPPVTSYGENIGVLAITRVHSVFVIGGAAVFAVILGFVGKL
                     SALILSIPGPVISGISFILFGVIAASGMKILIENKIDFDKKKNLLIASVILVVGIGGL
                     VFEVGTFTLSAMALATVLGIILNLILPETSRSEEN"
     gene            complement(13597..14136)
                     /gene="pyrR"
                     /locus_tag="ACG8KR_000555"
     CDS             complement(13597..14136)
                     /gene="pyrR"
                     /locus_tag="ACG8KR_000555"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HFQ0199957.1"
                     /translation="MQAKEVVDQVTMKRALTRITYEIIERNHSIQDIVLVGIKTRGIY
                     IASRIAERLKQLEDIDIPVGELDITLYRDDKKENPEEPELHSSDIPVSLEGKEVILID
                     DVLYTGRTIRAAMDAVMDFGRPRKISLAVLVDRGHRELPIRADYVGKNIPTSRAEEIL
                     VEMQELDGQDRIMILKEED"
     gene            complement(14371..15282)
                     /locus_tag="ACG8KR_000556"
     CDS             complement(14371..15282)
                     /locus_tag="ACG8KR_000556"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFQ0199958.1"
                     /translation="MTELKTTIHHESGRIDKVLTNLFTDYSRSQIQLWLKNGAVSVNG
                     QPVKANYKVKQGDEITVLIPEPETLDVVPENIPLTIVYEDEDVAVINKPQGMVVHPSA
                     GHTHGTMVNALMYHIKDLSTINGVIRPGIVHRIDKDTSGLLMVAKNDQAHESLAQQLK
                     DKTSLRKYVALVHGEIPHEKGTINAPIGRSKENRKMQAIREDGKSAVTHFTVLERFEG
                     FTLVELKLETGRTHQIRVHMKYIGYPLAGDPVYGPKKTLKGNGQFLHAQMLGFTHPTT
                     NEKMIFEAPLPEVFEKTLEKLRKNIAF"
     gene            complement(15269..15742)
                     /gene="lspA"
                     /locus_tag="ACG8KR_000557"
     CDS             complement(15269..15742)
                     /gene="lspA"
                     /locus_tag="ACG8KR_000557"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010765038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HFQ0199959.1"
                     /translation="MLIIYWIFSALLVGVDQLVKWWITDNLALGETKNLIPGILSLNH
                     IRNTGAAWSMLEGKMWFFTIVTLIAVVVILTLMIKNREKGNRWFMIGLSLILAGAIGN
                     FIDRIRLGYVVDMFQTDFMSFPIFNVADVTLVCGVICMLIYIILDEKDQRKNDRT"
     gene            complement(15747..16445)
                     /locus_tag="ACG8KR_000558"
     CDS             complement(15747..16445)
                     /locus_tag="ACG8KR_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HFQ0199960.1"
                     /translation="MANSDLFLISFNRIEKWMRDEMGNARNMGFTELVRRLAQRKQLM
                     IRKYEDDLLQLAQLRNAIVHDRIAVDFVIAEPNDWTVKRIQLIEQELIQPETVLPRFA
                     KRVTGFEQDLPLLELLKIVAEKRYSQFPLYNKGKFVALITLRNIGFWLAKESQKGPVD
                     LTNKRALDLIIQNGKYTNYHFVPAKTHIYEVEAMFREQGTLEAVLITKDGNPDGNLLG
                     IVRPRDIYKQIEKD"
     gene            complement(16432..16818)
                     /locus_tag="ACG8KR_000559"
     CDS             complement(16432..16818)
                     /locus_tag="ACG8KR_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0199961.1"
                     /translation="MKTTFIRKTGFYGMGSSIQLRINNEKRSVLSHEQKITLDLEVPF
                     TMQVTFYWLKSPVYTVKEAKPAYVITMNYLILQIYPFLFLATGLGAVFLQNLFFSFFA
                     VIVLIAFFFYIKNRAYLIKEENYGKL"
     gene            complement(16965..18635)
                     /locus_tag="ACG8KR_000560"
     CDS             complement(16965..18635)
                     /locus_tag="ACG8KR_000560"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HFQ0199962.1"
                     /translation="MKNDLQISQETELEPIVEVASKLDLKEDDLELYGKYKAKINFSG
                     INRSKDNAEGHLILVTSINPTPAGEGKSTITVGLGDALNKINKKSVIALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELNIDARR
                     VIWKRVVDLNDRELRKVIVGLGGPIQGVPREDGFDITVASEIMAILCLAADLEDLKAR
                     LARIVVGYTFDRQPVTAGDLKAEGALALLLKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSILATKTALRLADYVVTEAGFGADLGGEKFLDIKVPNLKKAPDAVVIVATVRAL
                     KMHGGMKKDELKNENLDALKVGFANLKRHIRNMEQYQLPVIVAINEFVTDTDNELTLL
                     EHLCENQGILAKRASVWADGAEGGVDLAKAVVRLIDRKEANYKPLYRLEETIQEKTET
                     IVKKIYGGNGVVFSKKAQKQIEEFTKNGWDKLPICMAKTQYSFSDDPSLLGAPEDFTI
                     TIREIIPKLGAGFLVALTGDVMTMPGLPKKPAALNMDVTNDGEVLGLF"
     gene            18818..19051
                     /locus_tag="ACG8KR_000561"
     CDS             18818..19051
                     /locus_tag="ACG8KR_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFQ0199963.1"
                     /translation="MTTGIVKWFDNKKGYGFISYDDTEEIFVHFTAIEEEGFKTLEEN
                     QVVEFEIIEGNRGTQAAHVKKGNVQTTDTSMHE"
     gene            complement(19150..19581)
                     /locus_tag="ACG8KR_000562"
     CDS             complement(19150..19581)
                     /locus_tag="ACG8KR_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HFQ0199964.1"
                     /translation="MKKNIRWQPELAHTIIYWSLTLIILFFSLILSLENTGPYWKSNF
                     VLGIFLVFLFLGLRRSFILKSNSVIIRYAAFWKDREISVGQIRSLTPTNTGVQMMLQD
                     EKAPSVFMMRKKDQVVLFDHFEKNGPKSESIPYPYTKKRKS"
     gene            19660..20073
                     /locus_tag="ACG8KR_000563"
     CDS             19660..20073
                     /locus_tag="ACG8KR_000563"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HFQ0199965.1"
                     /translation="MLKVYIDASTKGNPGPSGGGILIVYQGKQEQLSIPLSIGTNHQA
                     EFEVFLKTLEFLKKNDLQNETIFCFSDSKTLVSTVDKNATKNELFLPYLQRIQELLSE
                     FSLLILQWLPENKNKGADNLARQALHKQLKEKKTL"
     gene            complement(20111..21259)
                     /locus_tag="ACG8KR_000564"
     CDS             complement(20111..21259)
                     /locus_tag="ACG8KR_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFQ0199966.1"
                     /translation="MAKKQEEKKKQIPQQRTKKGVLAGILGLVTNLILFVSKLMIGLF
                     SGSVSIMADAVNSLSDTASSILTLIGFKIAAKPADQEHPYGHERFEYISGLFVSIIIT
                     YVGFQFLESSAEKIIHPENVSLTPIVFFVLIISIFLKFAQGKMYQVIAQSIDSETLRA
                     TATDSYNDVFTTLAVLLSAAIEWVTGWRIDGYIGFALALYILYSGLTMLRNFVYELLG
                     SRPTPAEIEEMEEHLNACDDILGYHDLLVHNYGPNKRFASVHIEVDESMNLNVAHKII
                     DLIEKDFKKSLDVELVCHLDPMPVDNEKYYNILRQLKQILLETGTGLNIHDFRVIHQG
                     KTLQFDVVVPEKFQFVDTELERILRSQIEEKIGDYQLEITFDHNYLLY"
     gene            complement(21381..21977)
                     /locus_tag="ACG8KR_000565"
     CDS             complement(21381..21977)
                     /locus_tag="ACG8KR_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFQ0199967.1"
                     /translation="MNSRNKTFRLVLRAILLAIIIVQAMVPWLGFIPLGFISLTIIHI
                     TVIIAAVVLGPKDGMVIGLFWGIATIVRAYAMPTTPFDTLVFTNPIISVVPRVLVGLV
                     AGLVFHWIYQKNKSITISSAFAGVLGSLVNTVLVLGFMGLFYTGATADAYGVDSALLF
                     KTLAGVAAINGIPEAIGAGIITPLLAKALFAATPLKPE"
     gene            complement(22177..24819)
                     /gene="alaS"
                     /locus_tag="ACG8KR_000566"
     CDS             complement(22177..24819)
                     /gene="alaS"
                     /locus_tag="ACG8KR_000566"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HFQ0199968.1"
                     /translation="MKQLSSAQVRQMFLDFFQSKGHTIEPSASLIPVNDPTLLWINSG
                     VATLKKYFDGSVVPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFKN
                     EAIHWAWEFLTDPKWMAFDPEKLYVTVYPKDTEAKRIWHEEVGLPLDHIIDVEDNFWD
                     IGAGPCGPDTEIFYDRGEAYNDLAEDDPENYPGGENERYLEIWNLVFSEFNHKPDNTF
                     EPLPHKNIDTGMGLERMVSIVQDASTNFETDLFMPIIHAVEKLSKQVKYGENPVTDIS
                     YKVIADHIRALSFAIGDGALPSNEGRGYVLRRLLRRAVMHGKKLGIEQAFLYRLVPVV
                     GEIMVSYYPEVLQQKEFIEKVIRTEEERFNETINEGLEILNQVIQEVKNENGTTLAGK
                     DIFKLYDTYGFPVELTEEVAEDEGLKVDHAGFEKEMDAQRERARSARSTEKSMGVQSA
                     LLTDIKVESKFIGYTEQESDSQLLVIVKGESLLNEVAEGEAQMIFDQTPFYAEMGGQV
                     ADQGWIMNEVGEIVAVVTDVQKAPNGQFMHTVEVKAPMSEGQTYHLLIDQSMRNRIIK
                     NHTATHLLHKALKEVLGEHANQAGSLVAPGHLRFDFTHFGQITSDELAEMERIVNEKI
                     WEAIPVETIETDIDTAKNMGAMALFGEKYGHDVRVVNIGGWSIELCGGTHVANTENIG
                     IFKIVSESGIGAGVRRIEAVTSKEAYELLEEEEKQLKQVATIVKSPQLKEVVSKAEQL
                     QQQVRELQKENEQLASKLANQQAGDVFKDPETINGKTIIAAQVKVKDMNQLRQLADQW
                     KQKNISDILVLANEQEGKVSLLAAMTKESNEAGLKAGDLIKAIAPKVGGGGGGRPDMA
                     QAGGKNPAGIPDALKAAKEWVAQQ"
     gene            25207..25638
                     /locus_tag="ACG8KR_000567"
     CDS             25207..25638
                     /locus_tag="ACG8KR_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0199969.1"
                     /translation="MKKIVGADEWIRAASCFLRYQVFVLEQGIPAELEFDGKDIPQQT
                     YYLFLDQKRPVATLRYEKKTPTCFKPERFCVAKEYRGKGLGGQLLEAAEKRAVSEGCQ
                     TSYVVAEMTAVDFYEKQGYHTCTEPFIEDEVLCIGMKKSLA"
     gene            complement(25683..27032)
                     /locus_tag="ACG8KR_000568"
     CDS             complement(25683..27032)
                     /locus_tag="ACG8KR_000568"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFQ0199970.1"
                     /translation="MNNFDIFRFKPFINEALTAKGFKQPTEVQERLIPLIAKGKNIIG
                     QSQTGTGKTHTFLLPLFEKINAEKEEVQVVITAPSRELAAQIYEAATQIAKFSTPEIR
                     VSNFVGGTDKQRQLNRLHHQQPQVVVGTPGRILDMMNEQALKIHTAFAFVVDEADMTL
                     DMGFLNEVDQIASRLPEKLQFLVFSATIPEKLRPFLRKYMENPIIEEIKPKAVISEDI
                     ENWLISTKGKDENQMIYQLLTIGHPYLAIVFANTKQHVDEIADYLKNQGLKVAKIHGD
                     ITPRERKRVMRQVQNLEYQYVVATDLAARGIDIEGVSHVINAEIPEDLDFFIHRVGRT
                     GRNGLKGTAITLYSPNDEQAIDAIEKLGVSFEPKEIKNGEIVKTYDRNRRAKREKSKD
                     VLDPTLIGLVKKKKKKIKPGYKKKIDWAIAEKNKKERKIERRQQSRTARKNKKNSHK"
     gene            complement(27102..28265)
                     /locus_tag="ACG8KR_000569"
     CDS             complement(27102..28265)
                     /locus_tag="ACG8KR_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFQ0199971.1"
                     /translation="MASYIEPIRFALIVFPFLALAISSVFFIYEYRKYGTFLVTRAVI
                     LYSFVFYLLCAYFLVILPLPAKETVAQMTGPKMELHLFASWQHFRDNTVLVLSEPSTY
                     LPAMKQSVFLEPAFNVLLLIPFGIYLRYYFRLSFMKTMIASFCLSLFFELTQLSGLYF
                     IYPRPYRLFDVNDLVTNTLGGIIGFVVTPLFTFMLPTRRRMDELSYEKGKTVSLTRRL
                     VAYLIDWTFLSLINLVAAIFFKIAFDSGAVTSHIWWLLLEVGVYFMIVPFITNGWTLG
                     KKIVRIRLVEEDRERISLKALMIRYGYLYFLFYGLSWLGQLLQQIMNSTTNTVLQMSA
                     VFISLGVLLLQGIFILNILLSFLRKKRILFYEKASHTYTVSTIGQTKDDDSRN"
     gene            28394..28717
                     /locus_tag="ACG8KR_000570"
     CDS             28394..28717
                     /locus_tag="ACG8KR_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0199972.1"
                     /translation="MLNMYFVFGVPIFLLFLYATIAYVRKRTTIHYLGFILLIISGFM
                     LVFNLQTWQQALLEMDKMTPHALSKVLGYPVYLIWLPIFISGCLVLLNIYRGVRRIVQ
                     LRKSK"
     gene            complement(28900..30249)
                     /locus_tag="ACG8KR_000571"
     CDS             complement(28900..30249)
                     /locus_tag="ACG8KR_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFQ0199973.1"
                     /translation="MEEYQATPVKEVKIENELKRTMGFFTALSTVMGTVIGAGVFFKA
                     ASVAEVTGSASLHMFSWFLGGMISVCAGLTGAELAAAIPETGGMIKYIERIYGNTAAF
                     LLGWAQVVIYFPANVAALSIIFGTQFVNLFGLSQSMIVPVAVTAAVSILLINFLGSKA
                     GGAFQSITLVCKLIPLFVIVIFGLFRQEGVDFQLFPIQAGENLSFFSALGAGLLATMF
                     AYDGWIHVGNISGELKKPAKDLPKAISLGIIGIMIVYLLVNAVFLRTASIDGVAGNSN
                     AASDVAKMIFGGFGGRLVTVGILISVYGTINGYTLTGMRLPYVMAKENNLPFSKLFAK
                     LHDKTKVPVAAGILELVIAIGMMMIGGFDTLTDMLIFVIWIFYTMVFVGVILLRKKEP
                     DLFRPYKVPMYPFIPLVAIIGGTFIVSSTLITQTFLASMGIVLTLAGIPIYLYLKRR"
     gene            complement(30318..31271)
                     /locus_tag="ACG8KR_000572"
     CDS             complement(30318..31271)
                     /locus_tag="ACG8KR_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HFQ0199974.1"
                     /translation="MKKVLVTGCLGQIGSELITRLRQELGSENVIATDIRMPEVIEKN
                     DIFEILDVMDYEKMRSIAEQYQVDTLIHLAALLSAVAEAKPQLAWNLNVTGLVNALEV
                     AREFQLKFFTPSSIGAFGPNTPKDHTPQDTLQRPTTMYGVTKVAGELLCDYYYVKYGV
                     DTRGVRFPGLISYATLPGGGTTDYAVDIYYSAIKEGKYTSFIDQGTSMDMMYMPDAID
                     AIIKLMNADPDRLVHRNAFNISAMSFDPEEIKASIQKIIPDFEMDYDVDPVRQSIAES
                     WPNSLDVSCAQEEWGFSPKYDLDVMTKDMLEKLKEKLVETV"
     gene            complement(31373..32563)
                     /locus_tag="ACG8KR_000573"
     CDS             complement(31373..32563)
                     /locus_tag="ACG8KR_000573"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HFQ0199975.1"
                     /translation="MSSKELTMYLEKELDQLKEKGLYNTIDILESENGACIIVDGKKM
                     INLASNNYLGFANREELKKACIEATETYGVGAGAVRTINGSLKIHQQLEEKIAEFKGT
                     EAAIAFQSGFNCNMGAISAVMTKEDAILSDELNHASIIDGCRLSGAKIIRIKHQDMKD
                     LEKKAKEAIESKKYKKIMYITDGVFSMDGDIARLPEIIPIVEKYGLITYVDDAHGSGV
                     TGKGAGTVKHFGLSDKIDMQMGTLSKAIGVVGGYVAGSKTLIDWLKARSRPFLFSTSL
                     TPGAAAAALASITLMQEHPELVEKVWENANYFKEELKKVGYNIGMSETPITPVILGDE
                     KVTQTFSKKLIEHGIYAKPIVYPTVPLGTGRIRNMPTAEHTREMLDEAVAVYQKIGKE
                     MEII"
BASE COUNT         9188 a         7223 c         5746 g        10653 t
ORIGIN      
        1 aaattctgtc acaacccctc ttttttgatc aacggtgttc aataaacagc gttctcatct
       61 gcttttctta gctctgctgg tccagtaacc gagtaagctc tttgataaat ctttgagcga
      121 tttcactgag cggacgtttc gccgaataaa tgatataaaa ttcatttagt tcgccttctg
      181 ccaaagggat taaccgcaag tgctcgcgaa tccctccaat cacaatacca gatcctgaag
      241 cgtaagcatc agtttcaaga acgatattca tcaaagtacc tcgatcactc gtataaatca
      301 ctgtttcttg atcagggatc tcgataaggt cttcataaaa atacgggaaa ttatttccat
      361 cctgtgtaaa acgaacttga ggataagata acaattcttc tttggtgatc tctgcgtgtt
      421 cagctaatgg atgtccttgc cttaaaaaaa tatgcgtttt gaattttcca atgctgcggc
      481 cacttagctc tccttgttgt aaatatcttc tgataccagc ttgattatgc tcattcatat
      541 aaataatgcc gatctcactg tgctgctctt tgacttcttc gatcactctc gctgttgtcg
      601 tctccatgat ccgaaattgt ttgatctcgt ctttgaattc ctcgagtagc tgtgcggtca
      661 aaggacctaa gaaatcataa tgctgggcag cgatcgaaaa attgtctctc ttttcactcg
      721 ttagatagtg agtttctaat gtatcaagct ggaccaatat tttctcggca cgatttaaga
      781 agtcttgccc ttcttctgtt aaatatgccc ctcgattcgt tcgagtaaac agcgtgatcc
      841 ctaactcggt ttctaattct ttgataccag ctgaaagact tggctgggag acaaacattt
      901 tctttgccgc ttctctgaaa ctaccattgt ttgctacagc gaccacgtat ttcatctgct
      961 gaatattcat tgctcaaact cctcgaaata ataaatttct cttccattca agggctggat
     1021 tggtcggttg ttcggtcgag catactgacc atcgaagggg agaagaaact cttggttcag
     1081 ttttcctaca tgatccatta caaaccattg gattggcccg ctggtaggcg gagttgtcaa
     1141 tgagccaata taatggaaat ggcttttttg ctccggaaag aacacatccg gatttaccca
     1201 atgctcatgt ttttctaagt tccaactttc cccattttct tgattataca gccaaccttc
     1261 ctgtaccatt agctggtaca tgacacccac aactagattc tcttttttgc tgttcatatg
     1321 aacaaaatga aattcaatat cttcctgttt tccgttgatg atatgttcag aaggcaagtg
     1381 gaaatgaata tctgtcaaat aatagcgttc tttcttgaac tctacataac tgagttggtc
     1441 aaaaggtact aaatgaatcg tgtttttgaa ttccttgtca gtaaaccgtt gcttttgata
     1501 atgaaaggaa agcgtgtctc cttctatttt ggtttcttcc cccttggtca aatgaatagg
     1561 cgactgcaat ggaaattccg cacctctcgc aaaccaatca cataaactcg cccaattttc
     1621 cggacctttg tctccagaat agccccattc gacatccata ttctttttca actatacttc
     1681 cccctgcatc tcatatcatt tttatttttc ttctttcttt acaatcgttg agacggtttc
     1741 taaaaacgcc tcttttccat aaaattcctg gttcaatagt tccggaaata tctctttaaa
     1801 gaaatactga acagaaggcc gatgttcaaa gcttttaatc gtagtcactg cctcaatgaa
     1861 catctcctta taaacttttt ccgggttatt tttgatgatt tcatcaaaag attcataaag
     1921 aaggtcaacc ttatctgaaa tggctaatag ttctccttct acggtttcat cttttccttc
     1981 gaacaaccga cggcgataaa cttcttggaa ctcagctgga atctcttggt tgatgaattc
     2041 gtccgtcatc ttttcttcaa ctgtttgcag catccctctt aactctttgc ttgcgtattt
     2101 caccggcgtt ttgatatcac caataaaacg ctctgtgtag tcatgattca atgatttttc
     2161 atagagcagt ttccaatcga tcggttgtcc attcgcttct tcaatgtctc ctagaacttg
     2221 cgcaatagat gctacacgat acgaatgtgc tgcaactgta tgctctgtat atttaaaaaa
     2281 tccaggtgcc cttgtgatcg tctctaaatt gttcaatcct aaaataaatt cattcaaccc
     2341 cataaaagtt acctcccttt gagatttctt tttccgaata tcatatcatt aaataactga
     2401 tattcgcaac atttccgctc tttaccattt ttgaaacagc tcccttacca aaaaatagag
     2461 aaaatctata tataatagaa aaaagagaag agattattct cttctttctg ctaaccagtc
     2521 ttagaaaaat gaattttaaa aaaatcaaaa actgatcacg atagaagttt cttttcatac
     2581 ctgatgaaaa gatgcttctc gcaatcagtt tttggatttt tgctattcca attgatgatt
     2641 ctggagacac aaatcggatt tctctatctt catcaagcgc gcttaaatct gccaattttc
     2701 aggatctttt ttccattctt ttagcagagt taattcttgc tcatcgatat aacgatcttc
     2761 taaagctgct tcaatcaata cagaataatt ggtcaacgtc ataagaggga tttcggcttt
     2821 ttcgaaattt gcttttcctt ttggcaactc atacgtaaag atagccgcaa caccaaggat
     2881 atccgcacct tctctttttg ctgcttcagc tgcttccaag acacttccgc ctgtagaaat
     2941 caaatcctcg atcacaacca tcttctgacc ttcaaatata cgtccttcga tttggttacc
     3001 tttcccatga tctttcgcct tacttcgaat atagaccatt ggcaaatcca gtatttccgc
     3061 tacccatgcg gcatgaggga ttcctgctgt tgcagttcca gcaatcactt gtacttcagg
     3121 gtaagcttct ttgattttgc ttgctaatcc ttttgcgatc tcttttcgga cttttggata
     3181 gctcatcgtg attcggttat cacagtaaat tgggcttttg attccacttg cccatgtaaa
     3241 aggattactt ggatttaaaa atactgcttc aatttctaat aagtcttttg cgatactttg
     3301 ttcaacattc attttaggtt agctccattc tgtttgaatc gtttgatatg ctttataagg
     3361 attagttgac tgagtgattg gtcgacctat aacgataaaa gttgagccga tcttttttgc
     3421 ctgagaaggt gtgactactc ttgtctgatc tcctgcgact gttccttctg gtcttatacc
     3481 cggagttaag caaacaaatt cgtctcctgc catttctttg atcgcttcta cttcccaagc
     3541 agaagaaacc acaccatcta atcctgcttt tttggcacag ctagcataat ggatcacact
     3601 tttttctaaa gaaacttcga ttagttgatc ggcatgcatt tcttcttcac ttgtagaagt
     3661 aagctgagtg atagctagaa gtagcggacg ttttccacct tctggagtcc cctcttctaa
     3721 tcctcgcatt gccgcctcca tcatacgaat accgccagcg gcatgaacac aggtgagatc
     3781 aacaccaagc tttgccaatc cccgcattgc tttttcgacc gtattcggaa tatcgtgaag
     3841 tttcagatct aaaaatacat tatgaccagc ctcttttaga taacgtacga tttcaggtcc
     3901 ttcttggtag aacaattcca ttccgatttt cacaaacaat ttttcttctt tcgggaaaaa
     3961 tcgaagaaag tcttctacct cttgcctagt tgaaaaatct aatgcaataa ttggtcgttg
     4021 gtctagttga ttcatcgttc acgttcctct ctcacttctt tgattaatct ttccagcgat
     4081 tcgatgccta gttcgctcat tcgcacaggg agctcttcaa ttagtttagg acagatatac
     4141 ggatcggtga aattcgcggt ccctatagct acagcacttg ctccagccat aaacatctca
     4201 agaacatcat ccactgtctg cacgccgccc atcccaatga ttggaaggct ggatatactt
     4261 gctacttggt gaatcaaacg aatagcgaca ggcttgattg ctggtccgga gagtccacct
     4321 gtttgattgg ctaaaatcgg ttttcttgtc ttaaggtcaa tccgcatacc aagaagagta
     4381 ttgatcatcg taaacccatc tgcccctcca gcttcgattg cctgagcgat tggaacgatg
     4441 tctgttacat ttggggacaa tttgacatag acaggaacag aagaaacttt ctttactgct
     4501 tgtgttaatt gaaaagcaac atcagaatct gttccaaaag caattccgcc atgtttgaca
     4561 tttgggcaag aaatgttcag ctcgattgct ttcacatttg gtgcttcgcc aattttgcta
     4621 caaacttcta catagtcttc ttcacaagca ccagcaacat ttgcgatgat gggtaactca
     4681 ttgtactcct ctaattctgg aagaagttta tgcataacaa cgtctagacc aggattttgc
     4741 aaaccaattg cattcaacat gccagatggt gtttccgcca ctcttggcgt gggattgcca
     4801 aagcgggcat ttggagtcgt tgccttgacc atgatcgatc caagctgatt caaatcatag
     4861 tattttccgt attctttgcc aaaaccgaaa cagccgcttg ccggcatgat aggatttttt
     4921 aattctaggc caggaagttt gaccgctaaa ggattctcat tcatataatt acctctccta
     4981 ttttaaaaat cggtccttcg tcacaaactt tcacactttt tgttcctgtt tcatcttccg
     5041 gcgtatgaca aacgcaagca tagcaagccc ctatcccaca tgccatgcgg gattctaatg
     5101 ataactggac attttgatgc gaagaaaaca gctgttccac tgtcttcaat aacccgtttg
     5161 aaccacatgc aaaaacagct gtaggttccg tcttcgttcc taacaataaa ttaccgacat
     5221 ttccgtgcat accgtatgtt ccatcgtcag tagcgattct tgtttctcct agacgattaa
     5281 attctttttc ataataaacg acttcatgtg tggcaaaacc taaaaaatgc gttggcttaa
     5341 ttcctttttc gactaactgt tttgataact catatagagg agggacaccg attcctccac
     5401 cgattataaa tacttcgtct cctgtatgaa gattggacaa atcaaaacca ttgcctaaag
     5461 gacccatgac atctaaatgg tctccggcag aaagagtaga gaagactcta gtcccctcac
     5521 cttccacccg ataaatgata cggcaagtat ttgtttcgtg gtcgatatga ttcaagctaa
     5581 tcggtcggcg taatagcaag tctgcacgcg gcacgagaat atgcaagaac tgtcctggtt
     5641 gctgcatttc ttttactaga tcacctttca atgtcatttc aaaaatcttt ggtgccagct
     5701 gtttttgctg aacgatttcc atcatttctt gtttcatctg tcccctcctg caacggtttt
     5761 tcgtcttttt gtcttaacat gtgctcgccg ttaaataggg ttagttgaaa aagcacggga
     5821 ttccatcact cgaacgatcg catctgctgt gtctaaagaa gtaaacaatg gaataccatg
     5881 ttcaactgct tctcttcgga tgatgaaacc gtctttagaa gcatttcccc gatctttatc
     5941 aatcgtattg acaaccactt gtgttttacc atctcttatt aagtcgacta catttttttc
     6001 tgctgcttcc tcagaaattt tcgctactgt ttcaacgacc agaccatttt tttcgaaata
     6061 agcagctgta tgtttcgttc ctatcagact atagccaatt tctgtaaaac gtttagctaa
     6121 ttctagtgct tcttcttttg tttcgtccgc aatcgtaaat aatacggctc catattcagg
     6181 caagtgtaat ccagaagctt cgaatgcctt gtacaaagct ttgtctaatt gtctgtcaga
     6241 tcccattact tctccagtcg atttcatttc aggccctaag taagtatcaa ctttctgcaa
     6301 tttcgtaaaa gaaaagacag gtgctttaac atgaacttct tttgtttctg gataaagtcc
     6361 gttttcataa ccaagtgtct ttagtttctc tcccaaaata gctttcgtcg caatttgtgc
     6421 cattggaatt cctgtcacct tacttaagaa tggaacagtc cgacttgctc gaggattgac
     6481 ttcgatcaca taaacctcat tttcatggat gacaaactgg atattcatca tcccgacaca
     6541 attcaaacct aatgctaagc ggatcgtgta atcttcgatc gttttcttga tcgattcaga
     6601 taatgtttga ggtggataca cagccattga atcccctgaa tggacaccag cacgctcaat
     6661 atgctccatg atccctggga tgagtactgt ttcaccgtca caaatcgcat ccacttcgca
     6721 ttcaccacca agcaagtaac ggtccactag tactggatgt tctggagatg ctttcacagc
     6781 atgttccatg taatcttcta aatctcgctg gttttcaacg atttccatcg cacgtccgcc
     6841 taatacatag cttggacgaa caagaactgg ataaccaatt ctcgaagcga taaccacggc
     6901 ttcttcttta cttgtagcag tatcccctaa aggttgagga atatccaact ctttcagcgc
     6961 ttgctcgaat aaatcacgat tttccgcacg atccaagtct tcgatcgttg tccctaagat
     7021 ttttacacca gcagcagtca aaggttcggc taagttgatt gctgtctgac caccaaattg
     7081 aacgattact cctatcggct gttctagctc gatcacattc atcacatctt cgaaggtcaa
     7141 tggctcaaaa tataatttat ccgaaataga aaagtcagtc gaaactgttt caggattgct
     7201 gttcatgatg attgcttcgt atcctgcctg ctggattgct ttgacagaat gcacagtcgc
     7261 atagtcaaat tcaacccctt gtccgatacg gataggacca gatcccagta ctagaattga
     7321 gggcttttct gatcgtgaac tttcattttc atattcatag gaactataaa aataaggtgt
     7381 ttttgattcg aattccgctg cacaagtgtc aaccatctta tatacaggaa tcaaatcatt
     7441 ttcttttctc atctggcgaa cggcttcttc gtttatttcc cacagctccg ccactttttt
     7501 gtctgtaaaa ccgttttgtt tcgctgtttt caatatcttg tcatccaacg gctgctgttt
     7561 caactgctgt tcgatttcta cgatatgcaa cagtttatct aaaaagaata aatcgatttt
     7621 tgtcaaatcc gctaattctt ctatggtgta ccctcttcta atagcttcag ctacataaaa
     7681 caggcgatca tcttgtgctc gaaccacttt ttggattagg tcatcgtccg tgatgtctgc
     7741 caactcagga agttcgatat ggtgtgtacc gatttctaaa gaacgcacag ctttcaataa
     7801 agactcttcg atattccgac cgatagccat cacttcacca gttgctttca tctgcgtgcc
     7861 taaaagccgt tctccgtttt caaacttatc aaacggccag cgaggaattt tagctacaac
     7921 ataatctaat gcaggttcaa attctgcaaa agtcgttcct gtaacaggat ttttcatttc
     7981 gtctaatgtt aatccaagcg ctattttagc agctagctta gcgatcggat agccagtcgc
     8041 cttacttgct aacgcagatg aacgagaaac acgcggattg acttcaatca cataataatt
     8101 aaaactatgt ggatcaagag cgagttggac attacagcct ccctcgatct taagtgctcg
     8161 aataatcgaa agagaagcat cacgaagcat ttgatattca tggtcagaca atgtttgact
     8221 aggggcaaat acgatagaat cacctgtatg gatacccacc gggtcaaaat tttccatatt
     8281 gcagacaacg attgcattat cagccgaatc tctcatcact tcatattcaa tttctttaaa
     8341 acctgcaata cttcgttcaa tcaaacattg ggtagcgggg gaaagactta atccattttc
     8401 tgatacgata cgtaattctt cttcattttc acacatacct ccaccagttc cgcctaaagt
     8461 aaaggctgga cgaacgatga caggatagcc gattttattt gcaaaatcta cagcttcttc
     8521 tactgtattg acgatttcag actcggggat tggctgattc aattctttca tcaactgttt
     8581 gaatagatca cgatcttctg cttgatcgat cgcagaaagc tttgttccta gaagttctat
     8641 gttcaattct tctaaaatac ctgatgctgc cagctccatt gccatattga ggcctgtctg
     8701 tccgcctaaa gttggaagga gagcatctgg acgctctttt cgaagaatcc ttgatacaaa
     8761 ttcaagtgtg atcggttcga tatatacttg atctgccact tctttatccg tcatgatcgt
     8821 cgcaggatta gaatttacta aaaccacttc atatccttct tcttttaagg caaggcaggc
     8881 ttgtgttcct gcgtagtcaa attctgcggc ttgaccaatg atgatcggac cagatccaat
     8941 caccataatt ttcttgatat ctgtacgttt cggcatctgt ttactgctcc tttcctgcgt
     9001 ccatcatttc cataaattca tcaaataaat gtagtgcatc atgcggacca ggggcagcat
     9061 ctggatgaaa ctgcacactg aaagcgggat aatctctgtg tttgactcct tctactgttc
     9121 cgtcatttac ttctacatga gttacgatga gtttttctgg atcaatcgtg gcttcatcga
     9181 ctgcatagcc atgattttga gaagtaaaat caattcttcc ggtcgctact tctcgtaccg
     9241 ggtgatttaa tcctcgatgt ccgaatttca ttttataggt atctgcaccg ttggctaaag
     9301 caaataactg gtgaccgaga caaataccaa aaatcggtac ttttccttgg atttttttga
     9361 tcatttcgat tgcttctggc acatcttttg gatcaccagg tccattcgtc agcatgactc
     9421 cgtctggtga tagcgctaaa atcgtctcgg catctgtgtt atagggaagt actgtgaggt
     9481 tacagtttct tttactcaat tctcgtaaaa tactatgttt caagccgaag tcaatgacaa
     9541 tgactttccg acctgttcct ggacttggat aaggtttgtt agttgagact tgctgcactt
     9601 gattggttgc tagaacggtt gctttcagct gatcaaatgc atgttcgaag gaatctccag
     9661 catcaactat actagctttc atcgttccga cttcacggat tttgcgagta agggcgcgcg
     9721 tatcgatacc tgatattcca ggaattcctt tttgtttcaa aaatgaatct agcgtcattt
     9781 ggtttcgcca gtttgaagct acacgtgcat gttcttttac tactactcct ttgcatgttg
     9841 gagaaatcga ttcataatca tctctattta ccccataatt acccaccata ggataggtaa
     9901 atgtaatgat ttgaccgtta aaactttgat cagtgatagt ttcttgataa ccagtcatac
     9961 ttgttgtaaa aacgatttct ccgaagacac tagcatctgc accaaaagcc tttccttcga
    10021 aaactgttcc atcttctaat ataagcaaac gttccatact tttattctcc cttctgccac
    10081 acaagttttc cgttgacaaa agtcatttgt gtttcgcctt ttactttcca gccgataaaa
    10141 ggtgtattct ccccttttga taaaaagtct tctttatcga tcgtgcaagt ttgtgtaata
    10201 tcaaaaactg ctacatcagc gggggcacca accgttagcg ttcctgcatt caaaccaaaa
    10261 atctcagccg gtttaactgc caaccaattg atcacttgtt ccaacgtgaa tcgaccagtt
    10321 tctacaaaat gagtatagat cagttgaaac gctgtttcgc ttcctacgat accaaatggt
    10381 gatttcatga aactttggct tttttcttct aatccatggg gggcatggtc tgtagcgatg
    10441 caatcgatcg taccgtcaag caagccttcg atcaaagctt ctctgtcctc ccgtcctctt
    10501 agcggcgggt tcattttcca aaagccgaaa tcttcgggaa tatcttcgtc gatcaaaata
    10561 agatgatggg gtgaaacttc tgctgttacg tggattcctg ctttttttgc gtctctgatt
    10621 acacggacac tttctttagt agaaacatgg caaacatggt aatgaacacc agttgcttca
    10681 gctagcaaca gatctctagc aatttgagaa gactcggtta cgcttaagat cccgggaaga
    10741 ccaagttctt ctgccttctt gccagcgtgc ataacgccgc caaataataa agattcatct
    10801 tctgtatggg ctactagggc tttattgttt tttgcagctt ctttcatagc cagatacatc
    10861 gtcccagctg tttggacacc tacaccgtca tttgtgaaag caaaagcacc ttcttcaatc
    10921 agtgcttctt gatcaactaa tttctcactt cgcagatttt ctgtgattgg cgcatactgc
    10981 aataccttca caactgcatc ttttctaatg atgtcatata cttgtctcag cttttctgcc
    11041 gtatcaggta caggatttaa attcggcatc gcgcatacag tagtgaaacc gccacgagca
    11101 gctgagcgag tcccagcttc gatcgtctcc ttgtatgtaa atcctggctc tctcaaatga
    11161 acatggacgt ccactaaacc aggtgtgatc aattgtccct ttgcatcaaa cacttcatca
    11221 aattcggctt ctgagaatcc tgtaccgatt gctttgattc gcccatcttc tatccaaatt
    11281 tctgctggtg tcgtcatatt ttttcgtgta ttgatctgac cgtttttaat gagtgttttc
    11341 attttttcat cctcctataa ttacgctttt ccatttagca ctgcttctaa gattgccatt
    11401 ctgacgaata ctccattgct catttgctga acgatccgcg actgtaatcc ttccactaat
    11461 gaatcagcta attctacatc acggttaact ggtgctgggt gcataatgat tgcatgtttt
    11521 tgcaacttct tcgcacgctc aactgttaac ccatgttcct gatggtagcc ttcttttgag
    11581 aaactttctt ttccatcatg gcgttcgtgt tgcacccgaa gcatcatcat gacatcaact
    11641 tgatctagta gttcatccaa tggcatataa tgtccgtaca cttcgaattc atcgtcgtac
    11701 cattctctag gacctgagaa gaatacttgt gcgcctagac gttttaacat ctgcatattg
    11761 gatttagcta cgcgagagtg agtgatatca cctacgatgg caactttcag accttcaaaa
    11821 tgaccaaatt cttcataaat ggtcatcaaa tcaagcaagc attgggttgg atgctgtccg
    11881 cttccgtctc ctccattgat aatcgaacac tggattgttt tactttgtat cagttcgtca
    11941 taatagttct catcaccatg acggataacc gctacgtcta cgcctaatgc tgacatcgtc
    12001 agtactgtat cgtatagtgt ttcccctttt tggacagagc ttgtgcttgc ttcaaattca
    12061 atcacttcta gccctagttt tttctccgct acatcgaagc ttttatgtgt gcgagtacta
    12121 ttttcgaaaa agaggtttgt agcaaagtac tggcgctttt gcggagtcca attagcacct
    12181 tgcttgaatt cctgccctct gcggatcaag cccataactt cttgatcagt aagtgcctca
    12241 acggtcaata aatgctttaa gctgatacgt tctgatttga tgatcataat gtttcctcct
    12301 tagttttctt cgcttcgtga agtttctggt aagataagat ttaaaatgat gccaagtaca
    12361 gttgctaatg ccattgccga caatgtaaat gtgcctactt caaagacgag tccgccaatt
    12421 cctactacta agataactga agcaatcaac agatttttct ttttatcaaa atcgattttg
    12481 ttctcgatta aaatcttcat tccacttgcg gcaattactc caaataagat aaaactgatt
    12541 cctgagatga ctggtccagg gatacttaaa atcaaagcgc ttaattttcc tacgaatcct
    12601 aaaatgactg caaatactgc agctccaccg atcacaaaca cgctatggac acgtgtgata
    12661 gccagaacac caatgttttc accgtaactt gttacaggag gacctccgac aaaggctgcc
    12721 acgatttgtg ctaatccatc acctgtcaac gtcttatgaa gacccggttc ttcaaagaaa
    12781 ttacgtttag ttagtttatt caataccata agatggccga tatgctctgt catcgtcaca
    12841 aaagcgatgg gtgccattgt tgtgattgct ccaagatata gctttggttc atattgtaca
    12901 aagggaatct caaagttagg aacagcaaac caggcagcgt ctacgattgg cttagtatcc
    12961 acgattccaa acagcaaggc tacgatgtat ccgctaataa tacctagtaa aatcggaatc
    13021 aatccgagaa aacctcttag ccacatgttg aagaaaattg ttaatcctaa tgtgatcagc
    13081 gctacagcaa tatacttgaa atcatacgtg ccctgattat acatagcatt tgttgctgca
    13141 tttgccgcaa gtcccaaccc gataaccatg atgactggac cgacgacgat tggaggcaag
    13201 attttatcca gccatgctga accgatcttt ttgatgatca gcgcaacaat caaatagact
    13261 aatccagttg tgactgctcc ttgtgcaatc gctggataac catctgtttt catcaaaagc
    13321 tgcatcgctg caatgaaagc gaagctgctt cctaagtacg caggtatctt tcctttagta
    13381 gtaaataagt acaccaatgt acctagtcct gaactgacta gcgcaatacc tggatcgata
    13441 cctactaaga tggggactaa cacggttgct ccgaacatcg tgaacaagtg ttgtaaactt
    13501 aatcctaccc aatgtcctgg ggttgggcga tcgtggatat ctaacaccac gtcatcatta
    13561 tgaaatcctt ttttctccga catgtttctc tctcctctaa tcttcctctt tcaaaatcat
    13621 gatgcgatct tgtccatcaa gctcttgcat ctctactaaa atctcttccg cccgagatgt
    13681 tggaatgttc ttcccaacat aatctgcacg gattggcaat tctcgatgcc cacgatcgac
    13741 caggactgct aatgaaattt ttcttggtct gccaaaatcc attactgcgt ccattgctgc
    13801 tcgaatcgtt cgtcctgtat acaaaacatc atcaatcaaa ataacttctt ttccttctaa
    13861 ggaaactggg atgtcagaag aatgaagttc cggttcttct ggattttctt ttttgtcatc
    13921 tcggtataaa gtgatatcca gttctcctac aggaatatcg atatcttcta gctgcttcag
    13981 gcgttcggca attcttgatg caatgtagat tcctcgtgtt ttgataccta ccaatacgat
    14041 atcttggata ctatggtttc tctcgataat ctcataagta attcgagtca atgctcgctt
    14101 catggttacc tgatctacta cttcttttgc ttgcattttc attcccccta cttttttgca
    14161 taaaaaaact cctgccctaa gaggacagga ggaatgttta gactgagcgt atactgatac
    14221 actgatagct atactatgtc aatgagcaat ctacttatgg cgcaccatac gtatccttgc
    14281 tcccaccctt cttagcctca cgggactaaa cttaaaggaa ttctattaag ttttatgctt
    14341 tcgttgttaa ttactatacc gttatctaaa ttaaaatgca atgttttttc ttaatttttc
    14401 taaagttttt tcaaaaactt ctggaagagg tgcttcaaag atcatctttt cgtttgtagt
    14461 cgggtgcgta aaacctaaca tttgcgcatg gagaaactga ccattgcctt tcaaagtttt
    14521 cttcggtccg tatactggat ctccagctaa cggataaccg atatacttca tatgcacacg
    14581 gatctggtgt gttcttccgg tttctagttt taattcaacc agagtaaatc cttcgaaacg
    14641 ttccaataca gtaaaatgtg tgacagccga cttcccatct tctcttatcg cctgcatttt
    14701 ccggttttcc ttcgaacggc cgattggcgc attgatggtt cctttttcgt gaggaatctc
    14761 tccatggact aaagcaacat atttgcgcaa agaggtctta tcttttaatt gctgagctaa
    14821 tgattcgtgt gcctggtcat ttttggcaac cattagcagt ccggaagtat ctttgtcgat
    14881 tcggtgaaca atacctggac gaataacgcc gttgatcgta gaaagatcct tgatatggta
    14941 catcagcgca ttgaccattg tgccatgcgt atgacctgct gaaggatgaa cgaccattcc
    15001 ttgaggtttg tttatcaccg ctacatcttc atcttcgtaa acgatcgtca acgggatatt
    15061 ttctgggacc acatctaatg tttctggttc ggggatcaag acagtaatct cgtctccttg
    15121 ttttactttg taatttgctt tgactggctg tccattgaca gaaaccgctc catttttcag
    15181 ccacagctga atttgcgaac gactgtaatc ggtgaataaa ttagtcaata ctttatcgat
    15241 cctaccagat tcatggtgaa tcgtcgtttt aagttctgtc attttttctt tgatcctttt
    15301 catctaaaat aatataaatt aacatacaaa tcactccaca tacaagtgtc acatctgcta
    15361 cgttaaaaat cggaaagctc ataaaatctg tctggaacat atctactaca tagccaagac
    15421 gaatacggtc gataaaattg ccaatcgcac cagctagaat caaactcaat ccaatcatga
    15481 accagcgatt acctttttca cgatttttaa tcatcaatgt gagaatcaca acgactgcaa
    15541 tgagtgtcac tatagtaaag aaccacatct tcccttctag catactccat gcggctccag
    15601 tgtttcgaat atgattcagt gacaaaatac ctggtataag atttttcgtc tctccaagcg
    15661 ctagattatc agtgatccac catttgacca actgatccac tcctactagc aaagcgctga
    15721 atatccaata aataattaac aaaaaatcaa tccttttcta tttgtttata aatatcacga
    15781 ggtcgtacga tcccgagcag attaccatcg ggattcccat ctttagtgat aagtacggct
    15841 tccaaagtcc cttgctcacg aaacatcgct tcaacctcgt aaatatgtgt tttagctgga
    15901 acaaaatgat aatttgtgta ctttccgttt tggatgatca aatccaacgc tcgtttattt
    15961 gttaaatcaa ccggaccttt ttggctttct tttgctagcc aaaacccgat attacgtaaa
    16021 gtaatcaatg ctacaaattt tcctttattg tataacggaa attgtgaata ccttttttct
    16081 gctactatct ttaacagttc aagtaacgga agatcttgtt caaaacctgt tacacgtttt
    16141 gcaaatcgag gcagcacggt ttcaggctga atcagttctt gttcgatcag ctggattcgc
    16201 ttgactgtcc aatcatttgg ctctgcaatg acaaaatcga ctgcgatccg atcatggacg
    16261 atcgcattcc ttaactgagc aagctgtaaa agatcatctt cgtattttct gatcattaat
    16321 tgttttcttt gagctaggcg tctgacaagt tcggtaaatc ccatatttcg tgcatttccc
    16381 atctcgtctc tcatccattt ttcgattcga ttgaaactga ttaaaaaaag atcagagttt
    16441 gccataattt tcctccttga tcaaatatgc ccggtttttt atatagaaaa agaacgcaat
    16501 caacacaata actgcaaaaa aactgaaaaa taaattttgt aggaaaacgg cccccaaccc
    16561 agttgctaga aataaaaacg gatagatttg aagaatcaag taattcattg tgatcacata
    16621 agcaggcttt gcttctttca ctgtataaac ggggcttttt agccaataaa aggtgacctg
    16681 catcgtaaag ggcacttcca gatcaagcgt tatcttttgt tcatgactga gaacactgcg
    16741 tttttcattg ttgattctta gctgaatcga gctacccata ccgtaaaaac cggtttttct
    16801 tataaatgtc gttttcatca taccctccta ttatgcttta gtatagctca aaatacaata
    16861 aagaaaaaga atgatcctag tgtaaacaaa attaattccc tatcgtctaa aaaaagaagc
    16921 tgtagtcaaa atacagctcc ttcgatggta gattataatc aagcttaaaa caatcccaac
    16981 acttctccgt catttgtcac gtccatattc aatgcagcag gttttttagg caaaccaggc
    17041 atcgtcataa catctccagt caaggctact aaaaatcctg cccctaattt tgggatgatt
    17101 tcgcggatcg tgatggtgaa atcttccggt gcacctagca gtgaaggatc atcagaaaat
    17161 gaatactgtg tttttgccat acaaatcggc agtttgtccc atccattctt cgtaaactct
    17221 tctatttgtt tttgcgcttt tttgctgaaa acaacaccat ttcctccgta aattttcttg
    17281 acgattgttt ctgtcttttc ttgtatcgtt tcttctaggc ggtataatgg cttgtaatta
    17341 gcttcttttc tatcaatcaa tcgtactaca gcttttgcta aatcaacgcc gccttctgct
    17401 ccatctgccc atacactcgc tctcttggca agaatcccct gattttcaca caagtgctcc
    17461 aacagtgtga gttcattgtc tgtatctgtt acgaattcat tgattgcgac gatcacaggt
    17521 agctgatatt gttccatatt acgtatatgg cgctttaaat ttgcaaaacc tactttcaaa
    17581 gcatcaagat tctcattttt aagttcatct tttttcattc cgccatgcat tttcaatgca
    17641 cggacagtgg cgacaatcac cacagcatcc ggcgcttttt tcaagttagg tactttgata
    17701 tctaaaaatt tctctccgcc aagatctgca ccaaatcctg cctctgtgac aacgtaatct
    17761 gctaatctca atgctgtctt ggtagctaaa atactattgc agccatgagc gatattggca
    17821 aacggtcccc cgtggacaaa agctggtgtt ccgtaaattg tttgtacaag attcggcttg
    17881 attgcatctt tcagcagtaa tgccagagcg ccttctgctt tcaagtctcc agcagtcaca
    17941 ggctgacggt caaatgtata gccgacaacg atccttgcaa gacgagcctt taaatcttca
    18001 aggtctgccg caagacacaa gatagccatg atttcgctgg caactgtgat atcaaaccca
    18061 tcttctcttg gaacaccttg gattggccca cccaagccaa caattacttt acgcagttca
    18121 cgatcattga gatcgactac ccgtttccaa atcactcggc gggcatcgat gttcaattca
    18181 tttccttgat gaatatgatt atctaaaaga gcagataatg cattattagc tgttgtgata
    18241 gcatgcatgt caccagtaaa atgcaagttg atatcttcca taggcaatac ctgtgcatat
    18301 cctccgcctg cagcaccacc tttgattccc atcacaggac caagtgaggg ttctcgcaat
    18361 gcaataacag attttttatt gattttattg agcgcgtctc ctaaccctac agtgatcgtt
    18421 gactttcctt cgccagctgg tgttggattg atggaagtaa ctaagatcaa atgcccctca
    18481 gcgttatcct ttgatcgatt gattccagaa aaattgattt tcgccttata tttcccatac
    18541 agttccaaat cgtcttcctt aagatctaat ttactagcca cttcaacaat cggttctagc
    18601 tctgtttctt gtgaaatttg cagatcattc ttcatttgat tacccctttt cacgaacttt
    18661 attcaacaaa atatcttctt tgttaagata tgccaattat accttttttt attcagaaaa
    18721 aaaacataaa attacaggtt tttaatttat ttgacgtact tataaatact ctttataatg
    18781 aaggctatga tcagacttta gaaaggtcgt gagtgctttg accacaggaa ttgtaaaatg
    18841 gtttgacaac aaaaaaggat acggctttat cagttatgat gacacagaag aaatttttgt
    18901 acattttact gcgattgaag aagagggatt caaaactttg gaagaaaatc aagtggtcga
    18961 atttgagatc atcgaaggga atcgaggaac acaagctgcc catgtcaaga aaggcaacgt
    19021 tcagactacc gatactagta tgcatgaata gataaacaaa tagagtgaga cgctaaacgg
    19081 gatgaaccgt acgtctcact cttttctttt cctgtaaaaa ttcgtttttt taggttctga
    19141 tttttaaaat tagctttttc tttttttcgt ataaggataa ggaattgatt ctgatttggg
    19201 tccgtttttt tcaaaatgat caaaaaggac tacttgatct ttttttcgca tcatgaaaac
    19261 agatggggct ttctcatctt gcaacatcat ttgcacccca gtattagttg gcgtcaaaga
    19321 tcgaatctgt ccgacagata tttctcggtc tttccaaaaa gcagcataac gtataatcac
    19381 agagtttgat tttaaaataa aagaacgccg caaacctaga aatagaaaaa caagaaaaat
    19441 acctaaaaca aaattgcttt tccaataagg acctgtgttt tctaaagaaa gaatcagact
    19501 aaaaaacaga atgatcaagg tcaatgacca atagatgatc gtatgtgcca gttcaggctg
    19561 ccatcgaata ttttttttca ttctctaacc tctcaccgat tcagtaatga tataatctta
    19621 ccataacatt agtttgaaca aaaaggagaa tctcttttta tgttaaaagt atacattgat
    19681 gcctctacaa aaggcaaccc tggtcctagt ggaggtggaa tcttgattgt ctatcaaggt
    19741 aaacaagaac agctatctat cccgctttct atagggacaa accatcaagc agagttcgaa
    19801 gtctttttaa aaactttaga atttctaaag aaaaacgact tacagaatga aacgattttc
    19861 tgtttttcag acagcaaaac actagtttct acggtcgaca aaaacgcaac gaagaacgag
    19921 ctcttccttc cctaccttca aagaatccaa gaacttctat ccgaattttc tttattgatc
    19981 ctgcaatggt tgcccgaaaa caaaaataaa ggagcagata atcttgcacg tcaggctctc
    20041 cataaacaat taaaagaaaa aaagactctt taaattaaaa actttaagga gtctttttat
    20101 agtttctcat ttaatataaa agataattgt gatcaaacgt aatttccaat tggtaatctc
    20161 cgattttttc ttctatttga ctacgtaaga ttctttctag ttctgtatca acaaactgga
    20221 atttctcagg gaccactaca tcaaattgca gcgtttttcc ttgatgaatt actctaaaat
    20281 catgaatatt aagccctgta cctgtttcaa gaagaatttg ctttaactgc cgcaagatat
    20341 tatagtattt ttcgttatca acaggcattg gatccaagtg acagacaagt tccacatcca
    20401 aagacttttt aaagtctttt tctatcagat caataatctt atgagccaca ttcaaattca
    20461 ttgattcatc tacttcgatg tggactgatg caaagcgttt attgggtcca tagttatgca
    20521 ccaataaatc atgatagcca agtatatcat cgcaagcatt taaatgctct tccatttctt
    20581 caatctctgc tggcgttggg cgacttccta atagttcgta tacaaaattt cgaagcatcg
    20641 tcaatccact ataaaggata tataatgcta aagcaaaacc tatatatcca tcaatacgcc
    20701 atccagttac ccattcgatt gctgctgaaa gcaatacagc cagtgtagta aagacatcat
    20761 tataactatc tgttgctgta gctctcaatg tttcagaatc aatggattgc gcaataacct
    20821 gatacatttt gccttgtgca aatttcaaaa agatggaaat gattaggaca aaaaagacga
    20881 tgggtgttaa agagacattt tctggatgga tgattttttc agcagaagac tctaaaaatt
    20941 gaaatcctac ataagtaatg atgatcgaaa caaataaccc actgatatat tcgaagcgtt
    21001 cgtgtccata aggatgttcc tgatctgctg gctttgcagc aattttaaat ccgatcaaag
    21061 tcaatatgga tgaagcagta tctgatagac tgttcactgc atcagccata atggagacac
    21121 ttcctgaaaa aagcccgatc attagcttag agacaaacaa gatcagattc gtaaccaatc
    21181 ctaagatacc cgctagtact cctttttttg tccgctgttg cggtatttgt ttcttttttt
    21241 cttcttgttt cttagccaag ttttttaccc cttctagttt gcttgttgct caggtaaaaa
    21301 gatggagagg acgattgttt tcctctcctc tcttcctttt tctatctatc gtgcgtccta
    21361 ttactattaa aagatggtca ttattctggt ttcaacggtg ttgctgcaaa taacgctttt
    21421 gctagtaatg gtgttatgat tcccgcacct attgcttctg gtatcccatt gattgctgca
    21481 acccctgcta aggttttaaa taaaagagca gagtctacac cataggcatc tgccgttgca
    21541 cctgtataaa ataatcccat gaatcctaac actaagactg tattgaccaa tgaacccagt
    21601 acccctgcaa aggctgagct gatcgttatt gatttatttt tttgataaat ccagtggaaa
    21661 accaagcctg ctactaagcc aacaagtact ctgggaacta ccgagataat cggattcgtg
    21721 aacactaatg tatcaaatgg cgttgttggc atggcatagg cacgaacaat tgtagcgatt
    21781 ccccaaaaca agccaatcac catgccatct tttgggccta atactactgc agcaataatt
    21841 actgtgatat ggataattgt taaactaatg aatcctaatg gaataaaccc cagccaagga
    21901 accatcgctt gtacgatgat gattgctaac aagatcgcac gtaagactag tcgaaaggtc
    21961 ttatttctac tattcatttt ttcacccctt tttatttata ctaatcgtaa aaatttctgt
    22021 cactttcgtc aactaaagac catccgaatc cgaccatttg attagatgcg ccatccaatt
    22081 gaaaaaagta tgagggcaag acttgtttgt cttcgccccc ataatcatgt ttcgttcaat
    22141 gtttgaactt ttgtttttct gtcaaacgta aacgccctat tgttgcgcaa cccattcttt
    22201 cgcagcttta agtgcatctg gaataccagc aggatttttc ccgccagctt gtgccatgtc
    22261 tggacgtcca ccaccgccac cgcctacttt cggtgcgatt gctttaatca agtcgccagc
    22321 ttttagtcca gcttcatttg attctttcgt cattgccgct agcagactga cttttccttc
    22381 ttgttcgttt gctaaaacaa gaatatctga aatatttttt tgtttccatt ggtcagctaa
    22441 ttgacgcagc tgattcatat ctttcacttt tacttgtgct gcaatgattg tttttccatt
    22501 gatcgtttct ggatctttga atacatcacc agcttgttga ttcgctaact tgctagctaa
    22561 ttgttcattt tccttttgta gctctcttac ttgctgttgc aattgttccg cttttgaaac
    22621 aacttctttc aattgaggtg atttaacaat tgttgctact tgttttagtt gtttttcttc
    22681 ttcttcaagt agttcgtacg cttctttact tgttacagcc tcgattcgtc gcacgcctgc
    22741 accaattcct gattcagaaa cgattttgaa gattccgatg ttttctgtat ttgctacatg
    22801 tgtgcctccg cacagttcga ttgaccagcc gccaatattt actactcgaa cgtcatgtcc
    22861 gtatttttca ccaaacaaag ccattgcacc catgtttttg gctgtatcga tatctgtttc
    22921 aattgtttca actgggatag cttcccagat tttttcgttg acgattcttt ccatttcagc
    22981 taattcatct gaagtgattt gaccaaagtg ggtaaagtcg aatcgtaaat gtccaggagc
    23041 gactaaagaa ccagcttgat ttgcatgctc gccaagtact tctttcaatg ccttgtgcag
    23101 tagatgggtt gctgtatggt ttttgatgat ccgattgcgc atcgattgat cgatcaatag
    23161 atgataggtt tgtccttcac tcattggtgc ttttacttct acggtgtgca taaattgacc
    23221 attaggcgct ttttgaacat ctgtaacaac tgctacgatt tcgcctactt cattcatgat
    23281 ccatccttga tccgctactt gcccacccat ttctgcatag aaaggtgttt gatcaaagat
    23341 catttgagct tcaccttctg cgacttcatt caaaagagac tcacctttaa cgatgaccaa
    23401 taattgacta tctgattctt gttctgtgta tccaatgaat ttactttcta ctttgatatc
    23461 tgtcaataaa gcagattgta cccccattga tttttcagta ctgcgggcag agcgagcacg
    23521 ttcgcgttgt gcatccattt ctttttcaaa accagcatga tcaactttca gtccttcatc
    23581 ttctgccacc tcttccgtta attctaccgg gaagccatat gtgtcgtata gtttgaagat
    23641 gtcttttcct gctagagtcg tcccgttttc gttttttact tcttggatca cttgatttaa
    23701 gatttccaaa ccttcattga ttgtttcatt gaaacgttct tcttctgtac ggataacttt
    23761 ttcaataaat tctttttgtt gaagtacttc aggataataa cttaccatga tttccccaac
    23821 aacagggact aaacgatata agaatgcttg ttcaatacct aattttttcc catgcatgac
    23881 agcacgacga agcaaacggc gtaagacata ccctctccct tcattcgatg gcaatgcacc
    23941 gtcgccaata gcaaatgaaa gggcgcgaat atgatctgcg attactttgt aagaaatatc
    24001 tgttactggg ttttcaccat attttacttg tttgcttagc ttttctacgg cgtggataat
    24061 aggcataaac aagtctgtct caaagttcgt tgacgcatct tgaacgattg agaccatgcg
    24121 ttccaatccc atccccgtat caatgttttt atgaggaagt ggttcaaatg tgttatcagg
    24181 cttatggttg aattctgaaa atactaagtt ccagatttct aaataacgtt cattttctcc
    24241 tcctggatag ttttcaggat cgtcttctgc taaatcgtta taagcttctc cacggtcata
    24301 gaaaatttcc gtatccggac cacatgggcc tgcaccgata tcccagaagt tgtcttctac
    24361 atcaataatg tgatctagtg gcaggccaac ttcttcatgc cagatgcgtt ttgcttctgt
    24421 atcttttgga taaactgtta cataaagttt ttctggatcg aatgccatcc attttggatc
    24481 agtcaaaaat tcccatgccc agtggatggc ttcatttttg aaatagtcac caatcgaaaa
    24541 attacccagc atctcaaaca tcgtatggtg ccgtgctgtt ttaccgacat tttcgatatc
    24601 atttgttcga atagattttt gggcatttgt gattcttgga ttttctggca caactgaacc
    24661 atcaaaatat tttttcaatg ttgcaacacc agagttgatc cacaataagg ttggatcgtt
    24721 cacggggatc aaagacgcac taggttcaat cgtatgtcct ttagattgga agaaatcaag
    24781 aaacatttgt cgaacttgtg cactacttaa ttgtttcatg tcattacctg ctttctataa
    24841 attaaataca aaaaaacacc atcacagcca aggacgaaaa ctcgcggtac caccttgatt
    24901 gcaatgatgt gtaatcatta cctcttaaac tcgataacgc tgagctgcgt cggtatttct
    24961 accaaaatag aaaaagggag caatttgatt agtgactatt cttttttcag ccaagaaaga
    25021 attttctgtg agtcttaaaa aacaaatcat atccttttcg tacttaatta gtatacgcat
    25081 tctattgatc actgtcaata tctcaatttt tattttcatt tccttttggt catactccac
    25141 gatttttggt acgataagaa taaaggaatc aatacatctc tgctattcat gacaaggagg
    25201 aatatgatga aaaaaatagt tggagcggat gaatggatac gcgcagctag ctgctttttg
    25261 agatatcaag tttttgtatt agaacaaggg attcctgcag aactagaatt cgacggaaaa
    25321 gatatcccgc aacaaaccta ttatctattt cttgatcaaa aaagaccagt agctactttg
    25381 cgttatgaaa agaaaacacc tacttgcttc aagccggaac gattttgcgt agcaaaagaa
    25441 taccgaggaa aagggttagg cggtcagttg cttgaagctg ctgaaaaacg ggcggtttca
    25501 gaaggttgtc agactagtta tgtagtagct gaaatgacgg ctgtagattt ttatgaaaaa
    25561 caagggtatc atacttgcac agaacctttt attgaagatg aagtactttg tatcggaatg
    25621 aaaaaaagtc tagcttaaaa acaagagatt gtgacacgcc ttttgtcaca acctcttgtt
    25681 ttttatttat gtgaattttt cttattttta cgagcagtac ggctttgctg tctgcgttcg
    25741 attttgcgtt cttttttatt tttttctgca attgcccaat cgattttttt cttataacca
    25801 ggcttgatct ttttcttttt cttcttcaca agaccaatca gcgtgggatc taatacatct
    25861 tttgattttt ctcgtttggc acgtctatta cgatcgtatg ttttgacgat ttccccgttt
    25921 ttgatttctt ttggttcaaa tgagacgccc aatttttcta tcgcatcaat ggcttgctca
    25981 tcatttggcg aatataacgt aatggccgtt ccttttaagc cattacgtcc agtacgtccg
    26041 acacgatgga taaagaagtc tagatcttct ggaatttctg cattaataac atgagagaca
    26101 ccttcgatat cgatccctct agcagctaaa tcagtcgcta caacatattg atactccaga
    26161 ttctgtactt gtcgcatcac tcgttttctc tctcttgggg tgatgtctcc atgaatcttt
    26221 gctactttca agccttggtt tttcaagtaa tcggcaattt catccacatg ctgttttgta
    26281 ttggcaaata cgattgctaa ataaggatga ccaattgtca gcagttgata aatcatttga
    26341 ttctcatctt taccttttgt tgaaatcagc caattttcaa tgtcttcaga aataactgct
    26401 tttggtttga tttcttcaat gattggattt tccatgtatt ttcgtaagaa tggacgaagt
    26461 ttttctggaa tagtggcaga aaaaactaaa aactgcagct tttctggtaa tcgacttgca
    26521 atttggtcta cttcatttaa aaaacccata tccaatgtca tatccgcttc atcgacaaca
    26581 aatgcaaatg cggtgtgaat cttcagcgct tgttcattca tcatgtctaa tattctacca
    26641 ggcgtgccta ctacaacctg tggctgttgg tgatgcagac gatttaattg acgctgtttg
    26701 tctgtaccgc cgacaaaatt cgagacacgg atctccggtg tagaaaattt agcgatttgt
    26761 gtagctgctt catagatctg tgcagctagt tccctacttg gagctgtaat aacaacttgt
    26821 acttcctctt tttctgcatt gattttttca aacaaaggca acaaaaacgt atgtgttttt
    26881 cctgtaccag tttgagattg cccaatgata ttttttcctt ttgcaattaa aggaatcaaa
    26941 cgttcctgga cctctgtggg ctgtttgaag ccctttgcgg ttaacgcttc attgataaac
    27001 ggtttgaagc ggaatatatc aaaattattc attcgtgcac ctcttctctt taaattatca
    27061 cgactttggc attataacac agacaaagcg tttttgctca gctaatttcg agaatcatca
    27121 tcctttgttt ggccaatcgt gctaacagta tatgtgtgac tagctttttc ataaaacaga
    27181 atccgttttt ttctcaaaaa tgatagcaag atatttaaaa taaaaatacc ttgtagtaac
    27241 aagacaccga gagaaatgaa tactgcactc atctgcaaca ccgtattcgt tgtcgaattc
    27301 atgatttgct gaagcaactg ccccaaccag cttaaaccat aaaaaagaaa gtacagatac
    27361 ccgtagcgga tcatcaatgc ttttaaagaa attcgttcgc ggtcttcttc caccaaacgg
    27421 atacgcacaa tttttttgcc aagtgtccat ccattggtta taaacggtac aatcataaag
    27481 tataccccta cttctaataa aagccaccag atatgagaag taactgcccc tgaatcgaac
    27541 gcaattttaa agaaaatcgc cgctaccaaa ttaataagag ataaaaatgt ccagtcgatc
    27601 aaataagcga ccaagcgtcg agtcaatgag acagtctttc ctttttcata agaaagttca
    27661 tccattcgtc ttcttgtagg aagcataaat gtaaataacg gcgtaacgac aaaaccaatg
    27721 attccaccca atgtatttgt cactaaatca ttcacatcaa ataagcggta tggtcttggg
    27781 tagataaaat aaagtccact tagctgcgtc aattcaaaaa atagagaaag gcagaaacta
    27841 gcaatcatcg ttttcataaa agaaagccgg aaataatatc ttaaataaat gccaaatggt
    27901 atgagtaata aaacattaaa agctggttcc aaaaatacac tctgcttcat tgccggcagg
    27961 taggtactag gttcagacaa gaccaatacc gtgttgtctc tgaaatgttg ccatgaagca
    28021 aataaatgca gctccatctt tggccctgtc atttgggcaa ccgtctcctt tgccggaagt
    28081 ggtaagatca ccaaaaaata cgcacacaat aaataaaaaa cgaaagaata caaaataact
    28141 gcccttgtta ctagaaatgt tccatacttt cgatattcat aaataaaaaa aacagatgag
    28201 atcgctaagg ctaaaaatgg aaaaacaatc agcgcaaaac gaatcggttc gatataactt
    28261 gccatatgac tcctccttga ttctttgtat ctctcttatc ttagcagtcc tttgctgttt
    28321 caagcaagta acgaggctat tatttctcat ttctttcaaa gtaaagtaag ataaacacaa
    28381 agggggtgtc gagatgctga atatgtactt tgtttttggc gtacctattt ttttactgtt
    28441 cctttatgct acgatcgctt atgtaagaaa aagaactaca attcattatt taggatttat
    28501 tctcctgatt atttcgggtt tcatgctagt cttcaatctt cagacttggc agcaagcact
    28561 tcttgaaatg gataaaatga ctccacatgc tttgtctaaa gtgcttggct accctgtcta
    28621 tctgatttgg cttcccattt tcatttctgg ttgcttggtt ttattaaata tttatcgcgg
    28681 cgtacgccga atagttcagt tacgcaaatc aaagtaaaag acagaaatcg gttgagcccg
    28741 atttctgtct tttactttgc tatttcatcg tagacaaaac gacatttacg tgatcaaaat
    28801 taacgagtac ttttgacatt tcttccacgt actcctcatt taaataaaat attttgtttg
    28861 gcagagaagt gcagttctaa taaaattcga atgttttagc tatctacgtt ttaaatagag
    28921 ataaatcggg ataccagcaa gtgtcaatac tatccccatc gaagctaaaa aggtctgagt
    28981 gataagcgtg cttgatacga taaaagtccc tccaatgatt gctactagtg gaataaacgg
    29041 atacattgga actttgtatg gtcgaaacaa atcaggttct tttttacgaa gaagaatgac
    29101 accaacaaag accatcgtat aaaaaatcca aatgacaaag atcagcatat ccgttagtgt
    29161 atcaaaacca ccgatcatca tcatgccaat agcgatgact agctctaaga tacctgcggc
    29221 tacaggaact ttagttttat catgaagttt agcaaagagc ttgctaaaag gcaggttgtt
    29281 ttcttttgcc ataacataag ggagacgcat tccagtaagg gtatagccat tgatggttcc
    29341 ataaacagaa atcaagatac caactgtgac cagtcttcca ccgaagccgc caaaaatcat
    29401 ttttgcaacg tcacttgccg cattgctgtt tcctgcgact ccgtcgatag atgcggttct
    29461 taagaacacc gcatttacta ataaataaac gatcatgatt ccgatgattc ctaaagaaat
    29521 tgcttttggc aaatctttcg ccggcttttt caattctccc gaaatatttc ctacgtgtat
    29581 ccaaccgtcg tatgcgaaca tcgttgctag caagcccgct cccaaggctg agaaaaatga
    29641 aagattttct cctgcttgga tcggaaacag ttggaagtct actccttctt gtcggaacaa
    29701 accgaaaatg acgatcacaa acaaaggaat aagcttgcag acaagcgtaa tcgactgaaa
    29761 agcccctcca gctttagaac ctaaaaaatt gatcaacagg atcgatacag cagctgtaac
    29821 agctacggga acaatcattg actgggaaag accaaataga ttgacgaact gtgtaccgaa
    29881 aatgatcgac aaggctgcta catttgctgg aaaataaatc acgacttgcg cccagcccaa
    29941 taaaaaagct gctgtatttc cgtaaatccg ttcgatatat ttaatcatcc ctcctgtttc
    30001 tggaatagca gctgccagtt cagctcctgt tagtcctgca cagacagaaa tcataccacc
    30061 gagaaaccaa gaaaacatat gcaaactggc ggatcctgta acttctgcta cgcttgcggc
    30121 tttaaaaaat acccctgcac cgatcactgt gcccataaca gtcgataaag cagtaaaaaa
    30181 tcccatcgta cgttttaatt cattttctat ttttacttct tttactggcg tggcttgata
    30241 ttcttccatc ccttgtcccc tttcatacca tagccaatcc caaaagtata aaaaactttt
    30301 tggatgtggt tcttgtttta taccgtctct accaattttt ctttcagttt ttccaacata
    30361 tcctttgtca tcacatctaa gtcgtatttc ggcgaaaagc cccattcctc ttgcgcacaa
    30421 gaaacatcta gtgaatttgg ccaagattct gctattgact gacgtacagg atccacatca
    30481 taatccattt caaaatctgg tatgattttt tgaatagatg ctttgatctc ttccgggtca
    30541 aaagacatcg ccgatatatt gaaggcattt cgatgaacca atcgatcagg atctgcattc
    30601 atgagtttta taatggcatc gatcgcatct ggcatataca tcatatccat tgatgtacct
    30661 tgatcaatga aactagtgta ctttccttct ttgattgctg aataataaat atcgactgcg
    30721 tagtctgtcg taccgcctcc tggcaaggtt gcataagaaa tcaatccagg gaaacggaca
    30781 ccacgagtat ctacgccata tttcacataa taataatcac atagtagttc tccagctacc
    30841 ttcgttaccc catacatggt tgtaggacgc tgaagagtat cttgtggggt atgatctttt
    30901 ggagtatttg gaccaaacgc gccgattgaa cttggcgtaa aaaatttcag ctggaattct
    30961 ctagcgactt ccaatgcatt gaccagccca gtcacgttca aattccaggc taattgtggt
    31021 tttgcttctg caacagctga caaaagagca gccaaatgaa tcaatgtgtc cacttgatat
    31081 tgttctgcta tgctgcgcat tttttcgtaa tccattacat ctaggatctc aaagatatcg
    31141 tttttctcaa tgacttcagg catgcgtata tctgtcgcaa tgacattttc actgcctagt
    31201 tcttgacgta gccgtgtgat caactctgaa ccaatttgtc ctaaacatcc tgtcaccaat
    31261 acttttttca tgatgatcct ccctgatctt ttttactact atcttcagat gattaacaat
    31321 atatgcgtga acatgttctc taattttaaa tagacgaaaa aactaaattt agttaaataa
    31381 tttccatttc ttttcctatt ttttgataaa ctgctactgc ttcatcaagc atttccctcg
    31441 tatgttccgc agtcggcata tttctgatcc tgcctgtccc aagaggcaca gttggataaa
    31501 cgattggttt agcataaatc ccatgttcga tcagcttctt agagaaagtc tgagtcactt
    31561 tttcgtctcc caaaatgact ggagtaatag gtgtttcaga cataccgatg ttatagccta
    31621 cttttttcaa ttcttcttta aaataattag cattttccca aactttttcg acaagttccg
    31681 gatgttcttg catcaaagta atcgaagcca atgcagctgc agctgcacct ggagttagcg
    31741 aagtagaaaa taggaatggc cgacttctag ctttcaacca gtcgattagt gttttcgatc
    31801 cagctacata acccccaaca acacctatag ctttcgataa cgtgcccatt tgcatatcga
    31861 ttttgtcgct taatccaaaa tgcttgacgg ttcctgcacc ttttccagtt acgcctgagc
    31921 cgtgcgcatc atcgacgtag gtgatcagtc catatttttc aacaatcgga ataatttccg
    31981 gcaatcgtgc aatgtctcca tccatggaga aaacaccatc tgtgatatac atgatttttt
    32041 tatatttctt actttctatc gcttcctttg cttttttctc aaggtctttc atatcttgat
    32101 gtttaattcg gatgattttt gctccggata agcggcaacc gtcaataata gatgcatggt
    32161 ttaattcatc cgataagata gcatcttctt ttgtcattac tgcagagata gcgcccatat
    32221 tgcagttgaa tccagattga aaggcgattg ctgcttctgt ccctttgaat tctgcgattt
    32281 tttcttctaa ttgctgatgg attttcagcg aaccattgat cgttcggact gctcctgctc
    32341 ctacaccata agtttcggtt gcttcaatac atgctttttt caattcttcg cgattggcaa
    32401 atccaagata attgttggat gctagattga tcatcttttt tccgtctacg atgatacaag
    32461 caccattttc actttctagt atgtcgatcg tattgtataa ccccttttct ttcagttgat
    32521 ccaattcttt ctctaaatac attgttagtt ctttggaaga cattgtctcc acctccatat
    32581 tcatctgtct tgcagaacat accaaacata tatcgtgtca ttcatctaca aagcaagtat
    32641 gctatatttt tgataacgct tacactgatt atgagaatta tttaaccaca ttttttattt
    32701 acagtatctt tttactttca aatgacttat atagtatgaa caattacctt ttagataatg
    32761 atctatttta ctctacctgg gcagataaat acaacacaac cattgataga
//
LOCUS       DAZKLD010000014        31321 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.014, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000014 DAZKLD010000000
VERSION     DAZKLD010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31321
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.014"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            271..1140
                     /locus_tag="ACG8KR_000574"
     CDS             271..1140
                     /locus_tag="ACG8KR_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFQ0199976.1"
                     /translation="MKDFQLGAATYLKSVAIRVKDRDKMIDFYKNVVGFDLKREENEL
                     AILGTEEAGSEMLLLEESPRANDHMGVIKKLNRFSLIIPSTEEMADILCRIRKMNYPI
                     KEALEDHGRMGILLVDPEENELEIYHEENKNDQREIPETMDQEALLRKSEEKFSKLST
                     GSYFEKIHLNVTNLTKERQFLNHVLGLKVHNEEEGIHVLNEGDFHVGLTEASGGAIDL
                     PTDEVIGLDFLKFMIDTESMEGLIAHLQQLSVDFFLDKKHTVLTIYDAIGIEWWFVSE
                     PKEKNKTDIRCDA"
     gene            1349..2374
                     /locus_tag="ACG8KR_000575"
     CDS             1349..2374
                     /locus_tag="ACG8KR_000575"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HFQ0199977.1"
                     /translation="MYPHTKQLILKKMEEGFYPGVAVAFIKENQVETFTAGFAQVVPE
                     KQLMTEDLLFDAASLTKVVATTSLVLRLLEDGKIELDQPLHKYLPEFENQTITLRHLL
                     THTSDIKTWIPNRDKLSQEELIAAYLKLEPGEQLGKEVKYTDAGTILLGLMLEEIYQE
                     TAVEIFQEQVLEPLGMMNSLFLPFPSKKIVPTEQLPSGEVLRGQTHDPKARVLAEHAG
                     NAGLFIDLKDSITFVQMYLNKGKIRNGRFLEEETIDSLLQDQTPKKNGYRSLGWDLKK
                     DVEDGHAILFHTGYTGTFFLIDIKTQEALIFLSNRVHPVDHRAEYLICRDEIVENFLK
                     EKAVLPK"
     gene            2413..3363
                     /gene="manA"
                     /locus_tag="ACG8KR_000576"
     CDS             2413..3363
                     /gene="manA"
                     /locus_tag="ACG8KR_000576"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HFQ0199978.1"
                     /translation="MQEPMFLKPVFQEKIWGGSRLRSVFGFDIPNDKIGEDWAISAHP
                     HGVSVVENGEFKGKRLDELWSEHKELFGHPSEPVFPLLIKILDAEDDLSVQVHPDDAY
                     GMKHEGELGKTECWYIIDAEPGAEIIYGHHAKTREELAEMIKDGRWDDLLKKVPVKKG
                     DFFYVPSGTIHAIGKGIMILETQQSSDTTYRVYDYDRKDAQGQTRELHIQQSIDVTTV
                     PAKTPELQIKEVRKGNSSIVTYLETEFFNVYEWDIKGITSFKKQAPYTLMTVIEGAGD
                     LVVDGKTYPLEKGTSCIIPNGVSEWAVQGELAIIASEPGK"
     gene            3457..5160
                     /locus_tag="ACG8KR_000577"
     CDS             3457..5160
                     /locus_tag="ACG8KR_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFQ0199979.1"
                     /translation="MEYKWKANNSSRVSMKQLRKAGHHLNEMIMPNLSLFIAWGILSL
                     ATKGVSGDLHTTLEEVCHWMIQLLLPILISYSGGKSVGGQKGAVAGAIASLGLIARSN
                     APQIVGAMIIGPLAGIVYEQFVSRFHVKFKAGYEMLVSNLLVGLTGSVICIIGIVFIE
                     PVLNSVHLLLASVVSWLVTMNLLPLVSLILEPLKVLFFNNAINHGVLTPLGIEFSQTV
                     GYSSLFLMEANPGPGLGILLSILCFAEKQEKVNASGALMIHAIGGIHEIYFPFVLLRP
                     YLFLAVMAGGASGTLIFQWLDTGLAAPVSPGSLLVIFANAPYPQLAGVSIGIAISTFV
                     TFICAAILLKLPNKEKENKTVKERGEILEPSIIHSVIFACDSGMGSSAMGAALLKKKL
                     AAEKIDLSVGYSSIYELNNQVNQLVIVQHELAGLAREKVPQSQIYVLDHFLETDILLS
                     RIKEAIQFPKREEKKEIADITEQKTSPANIVFLYKNNRRGSQTMGMTILSQLAKKAKK
                     QFVISKESIEKIKIQEETIYIAQRELAVTEQLEEKIPRLVLVEHWVATNEYEKLVKGE
                     I"
     gene            5162..7195
                     /locus_tag="ACG8KR_000578"
     CDS             5162..7195
                     /locus_tag="ACG8KR_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HFQ0199980.1"
                     /translation="MYLSPREKQLLAEFLSSPSPVSIQKMMNLLKVSKRTIYRELDNL
                     EVSLKSVNASLKKAARGSFVIEASEEVLEQLKENTDGKSNDLSTAKRQHAILAELLLT
                     TEPLSLAHFMESYRISNTTFYADIKQLEESIRQLPLEIIRNQGYEIEGPEKYRRLLTA
                     NVLELEINEYELFHSISFDASLNYFFRFVDPQHLSLARKVIGEELIQKKTNLSDRKLE
                     HLVLMLTLTMERVTKNHLLTNETYNSLANKELLQTAKRLFSRIASETKQLYPVNEIVF
                     FAGLLNDFTNSFDQNFFEETFDTQLAYLVKQLIQEVSEETNVHFYEDETLYKMLLTHL
                     SGVFSRAVLQEETLTNPILEKIMVQYEEVANAIRKAVERIFQEKKLSEEEIAYMVLHF
                     ANSLERSPKESNISIAGFSPSGLASTSMLELRLKRYFPFIQQIHFYRIADLKKIDLQE
                     EYDVVISTSILPGYGGKYILVSPLLLEDEVKQLKEAFSHIEKKKRQKITYNSNNEWMI
                     EDTYEETMHFMDQINQLLGRFFIQELDNPETISETIRSLFPYFPEGLVTDEEVVHRRL
                     LKRYEQAPIGIPHTNMGLFHTSSSLISKPIFCIFNLKHPLTIEGMDRQPVDLSRMLVM
                     LAPSPIQEKDGILLGKISGAIIMNDLNTEIFRSGNKEIVYQLLAKILIEEIKK"
     gene            7206..7646
                     /locus_tag="ACG8KR_000579"
     CDS             7206..7646
                     /locus_tag="ACG8KR_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFQ0199981.1"
                     /translation="MIQLPAEKILLDQSYQNWKEALTEIGQWMTQQGGIKPAYIQSMI
                     ERQEKASVYIGNFVALPHGTDKDEEVVEEGIYLVQVPDGVDFGTKEEPKIATLLFVVA
                     LKKEHQLAVLQDLAFFCSDIDQVMALSDAETIEEVQMIFEHGHI"
     gene            complement(7846..8973)
                     /locus_tag="ACG8KR_000580"
     CDS             complement(7846..8973)
                     /locus_tag="ACG8KR_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0199982.1"
                     /translation="MSLLTFERNLLLKNKVNFLFPLLLVVLFAFPLFFDHKLAYTEFD
                     ELNHNYEEMQRLIETLKEDENEKEFVESLEKSNKLIEAILHAKNTGNVQQMVEATYHY
                     EKDILDRLISGQRQGIPIIEQQKRVELLRYMKEHQIQRYSIFDLPAHLSLANYYENIF
                     SGMISSFLILCITALFLSSIISYEKRKQVISLVNLLPDSMVKKHSIRFTIYYGAAMLS
                     LVMPFLIVSILVIIKNGLGDFRYPVGTIIGQEIRILPMYEYLFQSFLFLLLWVLFLST
                     ISFLLSALFEHSLVNLLGTLLCLFLAEYRLFSSIGWIESISHYLPTSYVDFQNVIIGG
                     DIFSPLASEQVTFMNGILTLGIWSIVLLFIGMGTIYIKKSY"
     gene            complement(8976..9653)
                     /locus_tag="ACG8KR_000581"
     CDS             complement(8976..9653)
                     /locus_tag="ACG8KR_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFQ0199983.1"
                     /translation="MTLLIKNMNMKRGKKRVIRQLNLLVESGEVAALIAPNGFGKTTL
                     LNGIAQLLPTTYDQLSLHNISPTAYTDYRRSFFFLESTNQLSDYLSAVDYLTLIKYYW
                     QSKVECQTVFQRLKMTEYLTLPIGKMSLGMKQHVLLAAYLISDSSVMLFDEPLNGLDP
                     GSIDILNSLIRQWQKEGKTILLSSHAIANIQSICSKACFLKNGEIVTESSNMEEILAI
                     YRKLYEV"
     gene            9917..11683
                     /locus_tag="ACG8KR_000582"
     CDS             9917..11683
                     /locus_tag="ACG8KR_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol-specific transporter subunit IIBC"
                     /protein_id="HFQ0199984.1"
                     /translation="MERTIPKNNISLKARVQKLGSTLSSMVMPNIGALIAWGVLTALF
                     IPDGYLPNEAFATMVSPMLTYLIPLLIGYTGGKVIAGDRGSVVGAIATMGVIVGTDIP
                     MMLGAMIMGPLGGYAIKKFDQLFQKRIKSGFEMLVNNFSAGLIGFGLALLGFSAIGPV
                     VDALTQAMAKGVEIILSAHLIPLTSIFIEPAKILFLNNAINHGILTPLGTEQVAEAGK
                     SILFLLEANPGPGLGVLLAFTLFGKGAAKSSAPGAMIIHFLGGIHEIYFPYVMMKPLL
                     FLSVIGGGMSGSFIFQLMDAGLRAPASPGSIIAILAMTPFNSYLPVILGVIVAAAVSF
                     GISAVILKADAKDTNEDFEKSVQETKAAKQASKGAQGIPQGATFSGVQKIIFACDAGM
                     GSSAMGASILRKKVQEADLPQTVTNQAISQLTDDSNTLIVTQVELQERAKQKAPSARF
                     VSVENFLNSPRYDEIIQELSSKQKTSVSEIENEPAEEFLSANIKQIKEILLVHDDRSG
                     SATMGMTVLKDILDKNHLTIPLRKVNIQDLKDEPDSLIVAKESLAEKVQMAAPQSIHL
                     SVNSMVNPQKYESIAQYLKQTA"
     gene            11698..12135
                     /locus_tag="ACG8KR_000583"
     CDS             11698..12135
                     /locus_tag="ACG8KR_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFQ0199985.1"
                     /translation="MQSLPFDMIELNRSFKTKEEAIRYCGQMLVEAGCVDEEYIDAMV
                     DRDKMLSVYMGNFIAIPHGTDEAKKYVKKSGICVVQVPDGVDFGTEEEEKIATVLFGI
                     AGVGDEHLQLVQQIALYCSDMDNVVQLADALSKEEVTENLAIA"
     gene            12148..13299
                     /locus_tag="ACG8KR_000584"
     CDS             12148..13299
                     /locus_tag="ACG8KR_000584"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HFQ0199986.1"
                     /translation="MRAVHFGAGNIGRGFIGEVLAANGFEITFVDVNHTIIDALNERK
                     EYEIELADESKEKITVKNVKGINNQTNPEKVIQEIAEADLVTTAIGPNILPFIAELIA
                     KGIQEREKEGSTTSLDIIACENMIGGSAFLEKEVYKYLPETSFTDKYIGFPNAAVDRI
                     VPLQHHEDPLFVQVEPFKEWVIDDSQRKNKKIQLKGVLYVDDLEPYIERKLFSVNTGH
                     ATVAYTGALLGYQTIDEAMQDALVVAQLKSVLQETGSLLIAKWGFDAEQHHAYIEKII
                     HRFQNKNISDAITRVARTPLRKLGYQERFTRPVRELQEHNLTCPHLTATMGIIFNYYD
                     PEDEQSRQLHEMKIHENLEQLIQEVTGINDPKTIGNIKQNVNRYAKQVA"
     gene            complement(13361..14059)
                     /locus_tag="ACG8KR_000585"
     CDS             complement(13361..14059)
                     /locus_tag="ACG8KR_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HFQ0199987.1"
                     /translation="MANYELNIAHLYGNLMNTYGDNGNLLMLKYIAEKMGVSCHTEIV
                     SIHEPFDADKYDLVFFGGGQDFEQLIISKDIQKKKDSLINYIENDGVMLAICGGYQLL
                     GHYYMGANGEKIQGISALDHYTLSQENNRFIGDIVIHNEEFNETYYGFENHNGRTFLG
                     EGERPLGKVVQGQGNNGEDQSEGVIYRNVFGSYFHGPILARNENLAARLIRLALEQRY
                     GKELDLPYLEGQTA"
     gene            complement(14052..15404)
                     /locus_tag="ACG8KR_000586"
     CDS             complement(14052..15404)
                     /locus_tag="ACG8KR_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurT ligase domain-containing protein"
                     /protein_id="HFQ0199988.1"
                     /translation="MGIRSHFAIAVGKTSQWALKTFFKGGSSLPGKLALKVDPHILDS
                     LAKDYQVVVVTGTNGKTLTTALTVNILRQQFDEVLTNPTGANMVQGIVSTFLQAKPKK
                     GQKKFAVLEIDEASLSKVTEYMKPELFLFTNIFRDQMDRYGEIYTTYKMIVDGAAKSP
                     NATIISNGDSPIFNSIETVNPRKYYGFDHEEDREQMAHYNTDGVLCPNCHHILHYKMI
                     TYANLGKYYCPNCDFKRPELDYRLTDMVRMDNVSADFVIDGHEYGIEVGGMYNVYNAL
                     AATAVAEYFNVDPDKIKQGLGYDEKVFGRQEVININGKKCTLVLVKNPVGINQVIDMI
                     GLAPYPFSLVALLNANYADGIDVSWIWDGNFENFADMDIPAVISGGDRHTDMTLRLKV
                     AGIPENKLTETKELPEVIEKIKELPTEHVYILATYTAVLQLRKELAAQGFIKGGMNRG
                     "
     gene            complement(15627..17027)
                     /locus_tag="ACG8KR_000587"
     CDS             complement(15627..17027)
                     /locus_tag="ACG8KR_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HFQ0199989.1"
                     /translation="MHVDLFFRRLQRRGQKFAANHFSSIQIIVFYYILMTVLSLVLFY
                     MPIFREPDSHVSFVDMLFMAISTVSVTGLSTFDINSVFNDNGIILLEILFQVGGLGIM
                     MISTAFVIFSKRRITLKQRQLIMTDMNQPRLSGIVRLIRITFAILIWFQLLFGTFFSI
                     YFYYRGYFDRWRDAVFYGFYQAISAVTNSGFDVTGDSIKPFAHDYFFLILIMFLIFVG
                     GIGFPVLMECREWLLYKRSNAKLPFRFSLFTKLAVLAFVILFVSGTVLIYLLEKDHLF
                     QDSNMSVKWINSMFYSITTRNAGLQIHDLGDFQITTLIIFSLLMFIGCSPSSVGGGIR
                     TTTVAIIGLYLYSFLKSEDNINIFGRRIDQDDVRKSVVVFMLSLGMCFFCIVFLSATE
                     EQTLISIIIEVTSAFGTTGLSLGITGDLSVVGKITIATLMFIGRIGMLYTLMLFVPKE
                     TRDLGYEYPSEKIIIG"
     gene            17188..17925
                     /locus_tag="ACG8KR_000588"
     CDS             17188..17925
                     /locus_tag="ACG8KR_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HFQ0199990.1"
                     /translation="MINAQQIVEKMKNQKINYDTVLKKMIMQWEKEEVKPKILIHTCC
                     APCSTHTLEFMSQYAEITLFFSNSNIHPKSEYLRRLHEQKRFVDQFNEKTDHHVALIV
                     DEYRPNEFTKMVLTNHLENEKEGGARCSACFNMRLDLVAQKAQELGYDYFGSALTISP
                     KKNAALINQIGMDIQKIYGTQYLPSDFKKNKGYERSLQMCKEYDIYRQCYCGCVFAAK
                     QQGCDLKEINREAKAYLKTSTVVFEEN"
     gene            18050..18529
                     /locus_tag="ACG8KR_000589"
     CDS             18050..18529
                     /locus_tag="ACG8KR_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A-agglutinin attachment subunit precursor"
                     /protein_id="HFQ0199991.1"
                     /translation="MRKVAPILKPIAVLTISTIVATTTGIVNSSSYRAPSSSSSTVEE
                     TTPTTSVSTSEPTTTESLNETTTTSTWDDTTTSSSYDETYTTDSSSETSNTVPDGTPS
                     DSTNPSTSPDTSASSETIPSSTTVDDNTQNDDAARTAQQNQDSGNTQTYNQQAGDSQ"
     gene            18526..19293
                     /locus_tag="ACG8KR_000590"
     CDS             18526..19293
                     /locus_tag="ACG8KR_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6681 family protein"
                     /protein_id="HFQ0199992.1"
                     /translation="MTFLLDLINGVHKFLGYLDISPKYLNRGYTILSVIPTLYLLRIV
                     YGLWQNQNYLQFFLYGLAFLVLVYFTVLNVFYYFLDKNTKADVTQLFVKYLPDEAFNI
                     QTEESIDNGNSIDKVNTQEVMVSYDEDYQLKLAENMRYLIGNGEIKTNELGRMDGFLV
                     DRNTLYPYYYVKKTGEKEYVLNIGRSYSDMEKVGTIRLDSTEDTLEPVGLFIVGGDFV
                     KDGLRYHEPYRLKLIAKKAQSQEEPVVYSRSQRRRHS"
     gene            19523..20092
                     /gene="lepB"
                     /locus_tag="ACG8KR_000591"
     CDS             19523..20092
                     /gene="lepB"
                     /locus_tag="ACG8KR_000591"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFQ0199993.1"
                     /translation="MKEFLKNWGILILVLAAILLARVYVFTPVTVNGHSMDPTLSDGQ
                     RLISSKISNYERMDIITTKEPGDEERMIVKRIIGMPGDTVKMENDQLTINGKKYDEPY
                     LDEFKKEFSEDKLQGEYAYSSGFQAQAESSSTFTSDFEYTVPKGKYLVLGDNRLISKD
                     SRMFGLVDKDMIQGKVVFRYWPLSEIKIM"
     gene            complement(20196..20798)
                     /locus_tag="ACG8KR_000592"
     CDS             complement(20196..20798)
                     /locus_tag="ACG8KR_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HFQ0199994.1"
                     /translation="MTKNIFDSIANHYDSPDRQALAKIIREELTTYLPRDSHQKVLLD
                     YGGGTGLVSLPLAERFKELIIADASETMLKMAEEKIQAADLKNVRTIHADASVEFPAV
                     QANLILLSLVLLHIPDTENILTKLYEILAPGGQLIIVDFDKNEQISHPKVHNGFSQEE
                     LNNRLEKTGFVSATSHTFHRGEKLFMNKNASLFLSISQKA"
     gene            20904..21134
                     /locus_tag="ACG8KR_000593"
     CDS             20904..21134
                     /locus_tag="ACG8KR_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0199995.1"
                     /translation="MPISFVKDREEKGKCVREILLDLPEWFGLPESTEKYIEESSKLP
                     LWCEKRKEEYLGFITLSQTSEDTAEIYSIVWE"
     gene            21125..21373
                     /locus_tag="ACG8KR_000594"
     CDS             21125..21373
                     /locus_tag="ACG8KR_000594"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0199996.1"
                     /translation="MGIKKDYHHQGLGTKLFKEAEGYAAKHYKYLQVKTVDEGHYSIY
                     DQTICFYESLGFSRLEVFPNLWDEWNPCLVLVKKLEQK"
     gene            21479..22063
                     /locus_tag="ACG8KR_000595"
     CDS             21479..22063
                     /locus_tag="ACG8KR_000595"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HFQ0199997.1"
                     /translation="MAQLFFKYGAMNSGKTIEILKVAHNYEEQNKPVVLMTSGIDTRD
                     GLGVVSSRIGLKRNAMPIFEETDVFDVIQKMESKPYCVLIDEAQFLKKKHVIAFTRIV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLYADKIEEMKTICWFCHKKAIMNLHYIDGN
                     PVYEGDQVQIGGNEAYYPVCRKHYFHPSMITEGE"
     gene            22066..23139
                     /gene="prfA"
                     /locus_tag="ACG8KR_000596"
     CDS             22066..23139
                     /gene="prfA"
                     /locus_tag="ACG8KR_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HFQ0199998.1"
                     /translation="MYDQLQAIEDRYEELGELLSDPEVISDTKRFMQLSKEEANTRET
                     VEVYRRYKEVVQGITDTEELLGEKLDDEMAELAKEELSELKKEKEVLEEKIKILLLPK
                     DPNDDKNIIMEIRGAAGGDEAALFAGDLFNMYQKYAEAQGWKTEVMEANVTGIGGYKE
                     VIMMITGDNVYSKLKYESGAHRVQRVPSTESQGRIHTSTATVVVMPEAEEVEIDIEDK
                     DIRTDIYHASGAGGQHVNKTASAVRLTHLPTGIVVAMQDERSQIKNREKAMKILRARV
                     YDKIQQEAQSEYDANRKSAVGTGDRSERIRTYNFPQNRVTDHRIGLTIQKLDQILAGK
                     LDEIVDALIMYDQTSKLEAMQNG"
     gene            23132..23971
                     /gene="prmC"
                     /locus_tag="ACG8KR_000597"
     CDS             23132..23971
                     /gene="prmC"
                     /locus_tag="ACG8KR_000597"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFQ0199999.1"
                     /translation="MGKMTYREVLSRASSFLEDHGKEAYSIQFLFLERKQWKKLDWLL
                     HMNEEISEEEQRLIETDLRLLLADHPPQYLLGYADFYDHRLKVTEATLIPRPETEELV
                     EWCLDETPGVPLEVIDIGTGTGAIAISLKAARKNWHISAVDLSEEALEVAKENAQKEG
                     TKISFYHGDTLGPVMDQQFDVIISNPPYISRNEWELMDESVRSFEPKMALFAENDGLA
                     IYEKIAVEAPSVLKSNGKIFLEIGFRQGEAVKKIFQQAFPDKKVAIKKDLFGNERMIQ
                     VYG"
     gene            23964..24143
                     /locus_tag="ACG8KR_000598"
     CDS             23964..24143
                     /locus_tag="ACG8KR_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0200000.1"
                     /translation="MVEVVDEKFFICFFIIKKHLFDKDVNDFLVYQQVYPLLTNRLST
                     NSGKLEDKLRQNKNV"
     gene            24165..25190
                     /locus_tag="ACG8KR_000599"
     CDS             24165..25190
                     /locus_tag="ACG8KR_000599"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HFQ0200001.1"
                     /translation="METKRYDETELQEAAEALKEGELVAFPTETVYGLGANALLPNAV
                     KKVFSVKGRPQDNPLIVHVASFEQVKEYVDNFHPAAEKIVKNFWPGPLTLIFKIKKDT
                     LPSVVTGGLSTAAFRIPDNKKTLEVIELSGVPLVGPSANTSGKPSPTTADHVYHDLQG
                     KITGIIDDGATRIGVESTVLDLSDPTAMPMILRPGAVTKEQIEAVIESPVAIDQHLVK
                     ENETPKAPGMKYKHYSPDTRVLMVRDGDWSTAVQWAKNKKIRAGVIASPEIADQVRTD
                     TAAVYMYNDNSVEAAAKGLFAGLRGLDEPTLGLDLIFVQVYPETGLGNAYMNRLKKAA
                     GQNYFEK"
     gene            25365..26609
                     /gene="glyA"
                     /locus_tag="ACG8KR_000600"
     CDS             25365..26609
                     /gene="glyA"
                     /locus_tag="ACG8KR_000600"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HFQ0200002.1"
                     /translation="MVDYKTFDPDLWAAIAKEEERQEHNLELIASENFVSEAVMAAQG
                     SILTNKYAEGYPGHRYYGGCEFVDIVENLAIDRAKELFGAKFANVQPHSGSQANTAAY
                     LALVEPGDTILGMDLSAGGHLTHGSPVNFSGKTYHFVAYGVDPTTEVIDYNVVRILAR
                     KHQPKLIVAGASAYGRTIDFAKFREIADEVGAKLMVDMAHIAGLVAAGLHPNPVPYAD
                     ITTTTTHKTLRGPRGGMILTNDEALAKKINSAVFPGIQGGPLEHVIAGKAVAFKEALD
                     PAFKEYSEQIIANAKAMVKVFNQAIGTRVISGATDNHLMLIDVRELGINGKEAESILD
                     SVNITVNKNSIPFETLSPFKTSGIRIGTPAITTRGFKEEDAVKVAELVVKALQAKDDN
                     AQLDEVKTGVRELTEKFPLHKK"
     gene            26762..27709
                     /locus_tag="ACG8KR_000601"
     CDS             26762..27709
                     /locus_tag="ACG8KR_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase fold domain-containing
                     protein"
                     /protein_id="HFQ0200003.1"
                     /translation="MTDRISLEKEAVAFSDANAPHPRIYELAPEEGRVLLEEVQESPV
                     NKYSVDIEDLMVDTGEWGKLNIRLVRPEGNMDKLPVIFYIHGAGWVFGSAQTHDKLIR
                     ELAVRTNSVVVFPEYSRSPEAKYPTAIEQNYAVLQQLNKLAKSKNLDLENLIVAGDSV
                     GGNMATVMTILTKERKGLPISKQLLYYPVTNADFDTDSYHEFGENYFLTREGMMWFWD
                     QYTTNEEERAEITASPLRANINELNDLPSAMILTGEADVLRDEGEAYARKLRDAGVPV
                     TQVRFQGIIHDFVMVNAMDQTKATRAAMALSTSWIKKKE"
     gene            27843..28472
                     /gene="upp"
                     /locus_tag="ACG8KR_000602"
     CDS             27843..28472
                     /gene="upp"
                     /locus_tag="ACG8KR_000602"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HFQ0200004.1"
                     /translation="MGKFQVIDHPLIQHKLTIIRDKNCGTKVFREVVDEIAMLMAYEV
                     SRDMPLEDIVIETPITETTQKTLSGKKVAIIPILRAGIGMVDGILELIPAAKVGHIGL
                     YRDEETLEPHEYFVKLPEDIDARQLFVVDPMLATGGSAIMAIDALKERGASNIKFVCL
                     VAAPEGVKALQEAHPDIDIYTASLDEKLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(28611..28853)
                     /locus_tag="ACG8KR_000603"
     CDS             complement(28611..28853)
                     /locus_tag="ACG8KR_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0200005.1"
                     /translation="MKMATFGKFDTTIVPSEIGEKFKINTHVMFTIHGKSQTGTISKQ
                     LKNSAVVEIDETAENANLMTQSKGVVIVNYKELKKI"
     gene            28974..29046
                     /locus_tag="ACG8KR_000604"
     tRNA            28974..29046
                     /locus_tag="ACG8KR_000604"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:29007..29009,aa:Thr,seq:cgt)
     gene            complement(29165..29803)
                     /locus_tag="ACG8KR_000605"
     CDS             complement(29165..29803)
                     /locus_tag="ACG8KR_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family glutamic-type intramembrane
                     protease"
                     /protein_id="HFQ0200006.1"
                     /translation="MNNILGKKIELKKGILGTIVVVLGLLGLLFAPSIISIFSLTITG
                     FIAFTLVFGKKDTKKMFSKPVAPVKNIAKYFFINVVISAAVSVFLQQILKWGLTGNPI
                     NEAFSPLLFIILPIMILGEELFSVYFLAIFSSKFSIPVASFLSAIIFGFIHYSTYDNG
                     NILHTVAHILLIQGVARLLFNQAAIKSNSIITSWAVHVIFDFTAILLVVLFS"
     gene            complement(29926..30558)
                     /locus_tag="ACG8KR_000606"
     CDS             complement(29926..30558)
                     /locus_tag="ACG8KR_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFQ0200007.1"
                     /translation="MIFPKVNEASLNKGSFFHIYENEDFELYNILSQKEVIENQIICH
                     PIIYKSIFKYAFFASNDFQRMEITDCIFENCDFSGCILTKSMLHRVKFVNCKLTGTKL
                     MESYIGNTLFENCKMDYVNLSGSNIKESTFEHSVLSSADFVDCSLTKIMFSTNDLENA
                     SFFDTDLKNIDLSSNSFEMIEVRSEHLKNCTLNQLQALGFARKFLQIKVV"
     gene            30909..31208
                     /locus_tag="ACG8KR_000607"
     CDS             30909..31208
                     /locus_tag="ACG8KR_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFQ0200008.1"
                     /translation="MSKSEEIENMVLKLYDLVLNPEIKEKERILLIDAKTGLEKGQYY
                     PKVINNLERSLRPLAIRGELSKPVSPFYMEISTIGKFEKELGRGMASAPITFGHL"
BASE COUNT        10682 a         5473 c         6310 g         8856 t
ORIGIN      
        1 ccttaaacgg tgttcaaaag cttgcatctg cggtaataag cccaaccaaa cgaaaaatag
       61 gaggagctat ttttgtttgg ctgttcttat tacttgatgc agaacagctt tttcacaacc
      121 tctttttata agagttttta ctcattaatt tacaaaaaac aagcaaaatg cgaattttct
      181 cacatttttt ttaacaaaat caaagttatt gcttgaaaga agaagaatcc atcgttaaaa
      241 taaagcttga cgtagagggg agcaagaaat atgaaagatt ttcaattggg ggctgccacc
      301 tatttaaaaa gtgtagccat tcgggtgaaa gaccgagata aaatgattga tttttacaag
      361 aacgttgttg gctttgattt gaaaagagaa gaaaacgaat tagccatttt aggaacagaa
      421 gaagctggaa gtgaaatgtt gctgctggaa gaaagtccta gagcgaatga tcacatgggg
      481 gtgatcaaaa agctgaaccg tttttcattg attatcccta gcacagaaga aatggcagat
      541 atcctttgtc gtatccgtaa aatgaattat ccaatcaaag aagctttaga agatcatgga
      601 cgtatgggga ttttgctcgt agacccagaa gagaatgaat tggaaattta ccatgaagag
      661 aataagaacg accaaagaga aattccagaa acaatggatc aagaagcatt attaagaaaa
      721 tctgaagaaa aattcagtaa actatcaaca ggttcttatt ttgaaaaaat ccatttaaat
      781 gttactaact taacaaaaga acgccaattt cttaatcatg tacttggttt gaaggtacat
      841 aatgaagaag agggcatcca tgtattgaat gaaggagatt tccatgttgg tttgacagaa
      901 gctagcggag gcgcaattga tttgcctaca gatgaagtga ttggacttga ttttctgaaa
      961 ttcatgatcg atactgaaag catggaagga ctgattgctc acttgcagca actatcagtt
     1021 gatttcttct tagacaaaaa gcacacagta ctcacaatct atgatgctat aggaatcgaa
     1081 tggtggttcg tcagtgagcc aaaagaaaag aataaaactg acattcgatg cgacgcttaa
     1141 ctttttcttt cttaacgatt gtcgcatgtc tcgtcatttt atgaacggtc atactatcgc
     1201 ttattctaga gatagaaaga gaaaatagta taccggggga aactgcgcgg tttttcgcgc
     1261 agtttttttg tgtaaaaaag gttgattcct catagattaa gctttaagct tggactgtaa
     1321 gaatctgata gaaaagcagg agaaaaagat gtatccacac acgaaacagc ttattttgaa
     1381 aaagatggaa gaaggatttt atccaggtgt agcagtcgcc ttcataaaag aaaatcaagt
     1441 agaaacattt acagccggct ttgcccaagt cgttcctgaa aagcagctga tgacagaaga
     1501 tctgttgttt gatgcagcct cactgacgaa agtcgttgca accacttcat tagttttacg
     1561 tctattagaa gatggaaaga tagaactcga tcaaccgctt cataaatatc ttcctgaatt
     1621 tgaaaatcag acaatcactt tgcgtcattt actgacacat acttctgata tcaaaacatg
     1681 gataccaaac agagataagc tatctcagga agaattgatc gccgcttatt tgaagctaga
     1741 accaggtgag caactaggaa aagaagtaaa atatacagat gccggtacga ttcttcttgg
     1801 gttgatgcta gaggaaattt accaagaaac tgcagtggaa atctttcaag aacaagtatt
     1861 ggaaccatta ggaatgatga acagcttatt tcttcctttt ccttccaaaa aaatcgttcc
     1921 cacagaacaa ctgccttcgg gtgaggtttt gcgtggacaa actcatgatc cgaaagcaag
     1981 agttttggct gaacatgcag gtaacgcggg tctctttatt gatttgaaag acagcattac
     2041 ttttgtccaa atgtatttga acaagggaaa aatccgtaat ggtcgttttt tagaagaaga
     2101 gacgattgat tctttactgc aagatcaaac accgaaaaaa aatggctatc gttctttagg
     2161 atgggactta aaaaaggatg tagaagacgg tcatgcgatt ctttttcata ctggttatac
     2221 aggtacgttt ttcttgattg atataaaaac acaagaagcg ttgatatttt tgtcaaatcg
     2281 cgtacatcct gtcgatcatc gtgcggagta tctcatatgt cgagatgaaa ttgttgaaaa
     2341 ctttttgaaa gagaaagcgg tattaccgaa ataatgctat aatagctatg aaaaatgaga
     2401 ggagtttgaa ttatgcagga acctatgttt ttaaaaccag tttttcaaga aaaaatttgg
     2461 ggaggaagtc gtttacgttc agtctttggt tttgatatcc ctaacgataa aatcggagaa
     2521 gattgggcga tcagtgcgca tcctcatggt gtcagtgtag tagaaaatgg cgaattcaaa
     2581 gggaaaagat tagatgaact atggtcagaa cacaaggaat tgtttggcca tccttcagaa
     2641 ccagtgtttc cattattgat caagatctta gacgcagaag atgatctatc tgtacaagta
     2701 catccagatg atgcctatgg gatgaaacat gaaggtgaat taggaaaaac tgaatgctgg
     2761 tatatcatcg atgctgaacc tggtgcagaa atcatctatg gtcatcatgc aaagacaaga
     2821 gaagaactag ctgagatgat caaagatggg cgctgggatg accttttgaa gaaagtacca
     2881 gttaaaaaag gtgatttctt ttatgtacca agcggaacga tccatgctat tggtaaagga
     2941 atcatgattt tagaaacaca gcaaagttct gacacgactt accgcgtgta tgactacgac
     3001 agaaaagatg cgcaaggaca aacaagagag ctgcatatcc aacaatcgat cgatgtaaca
     3061 actgtccctg cgaaaacacc agaattacaa atcaaagaag tccgaaaagg gaattcatcg
     3121 atcgtcactt atttagaaac tgagtttttc aatgtatatg agtgggatat taaaggaatc
     3181 acatctttca aaaaacaagc cccttataca ttgatgacgg taatcgaagg agcaggagac
     3241 cttgtcgttg acggaaaaac atatccatta gaaaaaggaa caagctgtat tattccaaat
     3301 ggcgtttctg agtgggcggt ccaaggtgaa ctagcaatca ttgcgtctga accaggcaaa
     3361 tagttcccag atagctgaaa gcagcagacg gtttttcgtt tgctgctttt tctacataaa
     3421 atccgtccat caataaatag aagtctggtg aaaaagatgg aatacaaatg gaaagcaaac
     3481 aacagcagtc gcgtgtctat gaaacaactg agaaaagctg gacatcactt aaatgaaatg
     3541 atcatgccaa atctaagtct gtttatcgct tggggaatct tgtcattagc aacaaaaggt
     3601 gtgtctggcg atttgcacac cacattagaa gaggtatgcc attggatgat ccagctgctt
     3661 ttacccatct tgatcagtta ttcaggcggg aaatcggtag ggggacaaaa aggggcagtc
     3721 gccggtgcaa ttgcttcttt agggttgatc gctcgttcca atgctccaca aatcgtagga
     3781 gcgatgatta tcgggccgct tgccggcatc gtatatgaac aatttgtttc tagattccat
     3841 gtgaaattca aggctggata tgaaatgctt gtcagcaacc tactagtagg actgactggt
     3901 agcgttattt gtataattgg aatcgttttt attgaaccag ttttaaatag tgttcacttg
     3961 ttgctcgcct ccgttgtgtc ttggctagtc acaatgaatc tgttaccact tgtcagtctg
     4021 atcttagaac ctctaaaagt actatttttc aataatgcaa tcaatcatgg tgtattgaca
     4081 cctttaggaa tcgaattctc acaaacagtc gggtactctt ccttattttt gatggaggca
     4141 aatcctggac ctggtttagg gatcttactt tctatccttt gttttgcaga aaaacaggaa
     4201 aaagtaaatg cgagtggagc attgatgatc catgcgattg gcgggattca cgaaatctat
     4261 tttccatttg tcttgctgcg accgtatcta tttttagctg taatggctgg tggggcaagc
     4321 ggcacgttga tatttcaatg gttagatacg ggattagctg cacctgtatc gcctgggtca
     4381 ctactcgtga tttttgccaa tgcgccttat ccacaactag ctggggtaag tatagggata
     4441 gcaatcagta catttgtgac cttcatttgt gcagcgatac tattgaaatt accaaataaa
     4501 gaaaaagaga acaaaacggt caaagaaagg ggagaaatct tggaaccatc tatcattcat
     4561 tccgtcattt ttgcttgcga ctcaggtatg ggatcaagtg ctatgggagc cgccctgctg
     4621 aaaaagaaat tagcagcaga aaaaatcgat ctttctgtcg gatacagctc tatctatgaa
     4681 ttgaataacc aagtaaatca gctggttatc gtgcaacatg aactagcggg tcttgcaaga
     4741 gaaaaagtac ctcaaagtca aatctatgta ctggatcact ttttggaaac agatatcttg
     4801 ctgtcacgaa taaaagaagc gatccaattt ccaaagcggg aagaaaaaaa agagatagca
     4861 gatatcacag aacaaaaaac gtcgcctgct aatattgtat ttttgtataa aaacaacaga
     4921 cgcggctcac aaactatggg catgacgatt ttaagtcaat tagcaaaaaa agcgaaaaaa
     4981 cagtttgtta tctcaaaaga atcaatagaa aaaatcaaaa tccaagaaga aacgatttat
     5041 atcgcacaaa gagaactagc tgtaactgaa cagctagaag aaaagatccc tcggctagtt
     5101 ctagttgaac attgggtagc tacgaatgaa tacgaaaaac tcgtgaaggg agaaatataa
     5161 gatgtattta tcgcccagag aaaaacaatt attagcagaa tttctcagta gcccgtcacc
     5221 tgtatctatc caaaaaatga tgaatttatt gaaggtcagt aaacggacta tttaccgcga
     5281 attagataat cttgaagtct cattgaaatc ggtaaatgct tctttgaaaa aagcagctag
     5341 aggctcattt gtcattgagg catcagaaga agtattagag caattaaaag aaaatacaga
     5401 tgggaaaagt aatgacttat ccactgctaa acgacaacac gcgattttag ccgagttgtt
     5461 actgacgaca gagccgttga gtcttgctca tttcatggaa tcatatcgca tcagtaatac
     5521 cactttttat gcagatatca aacagttgga agaaagtatt cgccaactgc ctttggaaat
     5581 cattcggaac cagggatacg agatcgaagg accggaaaaa taccgccggc ttctgacagc
     5641 gaatgtatta gaattagaaa tcaatgaata tgaacttttt catagtattt ctttcgatgc
     5701 atcactgaat tatttttttc gattcgttga tccgcaacat ctatctcttg cacgcaaagt
     5761 aatcggggaa gaattgatcc aaaaaaagac caatctaagt gatagaaagc ttgaacattt
     5821 ggttttgatg ctgacattga cgatggagcg tgtgacgaaa aatcatttac tgacaaatga
     5881 gacatataat agtctggcaa acaaagaatt actacagaca gctaaacgat tgttttcacg
     5941 gatcgcttct gagacaaaac aactttatcc agtaaatgag atcgtctttt ttgcaggatt
     6001 attaaatgac ttcacgaact catttgatca gaatttcttt gaagagacgt tcgatacaca
     6061 gcttgcttat ttagtaaaac aactgattca agaagtaagt gaagagacga atgtccattt
     6121 ttatgaagat gaaactttgt acaaaatgct gttgactcat ttatctggtg tgttttcaag
     6181 agccgtttta caagaagaga cactgaccaa tccgatttta gaaaaaatca tggttcaata
     6241 cgaggaagtt gcgaatgcaa tccgtaaagc agtagagcgg atttttcaag aaaaaaaatt
     6301 atccgaagaa gagatcgctt atatggtcct tcattttgcg aattcattgg aacgaagtcc
     6361 gaaagaaagc aatatcagta ttgcaggatt ttcgccaagt ggattagctt ctactagcat
     6421 gttggaacta cgattgaaaa ggtattttcc cttcatccaa caaattcatt tctatcggat
     6481 tgctgattta aaaaaaatcg atttacaaga agaatatgat gttgttattt ctacatctat
     6541 tttaccagga tacggtggaa aatatatttt agtctccccg ctactgctag aagatgaagt
     6601 caagcagcta aaagaagctt tttcgcatat tgagaagaaa aaaaggcaaa aaattacgta
     6661 taacagcaat aacgagtgga tgatagaaga tacttatgag gaaacaatgc atttcatgga
     6721 tcaaatcaat caattactcg gtcgtttttt tatccaagaa ctggataatc ctgagacgat
     6781 ttccgaaacg attcgcagct tatttcctta ttttcctgaa ggactagtaa cggatgaaga
     6841 ggtggttcat cgccggctgc ttaagcgata tgaacaagca cctatcggta ttcctcatac
     6901 aaatatgggg ttattccata catccagctc attgatttct aaaccgatct tttgtatttt
     6961 caatttgaaa catccgttga ccattgaagg gatggataga cagccagtcg atttgtcccg
     7021 tatgttagtt atgctggcgc cttcgccaat ccaagaaaaa gacgggattc ttttaggaaa
     7081 gatcagtgga gcaatcatca tgaatgactt gaacaccgag atcttccgct caggaaataa
     7141 agaaatcgtt tatcagttgt tagcgaaaat cctgattgaa gaaataaaga aatgaaggtg
     7201 aaaagatgat acagctacca gcagaaaaaa tattgctcga tcaatcctat caaaattgga
     7261 aagaagcgtt gaccgagatc ggccagtgga tgacccaaca agggggaatc aaaccggcat
     7321 atatccaatc aatgatcgaa agacaagaaa aagcgagtgt ctatatcggg aatttcgtag
     7381 ccttgccaca tgggactgac aaagatgaag aagtagtaga agaaggaatc tatttagtcc
     7441 aagtgcctga tggtgtggac tttggaacaa aagaggaacc aaaaatcgcg acactcttat
     7501 ttgtagtggc tttaaaaaaa gaacatcagt tagcggtgct ccaagattta gcctttttct
     7561 gttcagacat cgaccaagta atggcgctca gtgatgcgga aaccattgaa gaagtccaaa
     7621 tgatttttga acatggacat atataaccaa tgttcacagt aaaaaaagct gccttgcatc
     7681 aagtaataac ttcaaccaag ataaaaataa gaggagctat ttttgttggg aggttcttat
     7741 tactgcggat gcaagctttt gaaaactatt tattctagaa aaccgtttat ttgaaatagg
     7801 ggacggtgac aaggtttttg tcaccgcccc tattattttt gtgttttaat agctcttttt
     7861 tatatagata gtgcccatac ctatgaataa taaaacgata ctccatatac caagtgtcaa
     7921 gataccattc atgaaagtaa cctgttctga agctagtggc gagaaaatat ctcctcctat
     7981 gatcacattt tgaaaatcaa cataagaagt tggcaagtaa tgactgattg attctatcca
     8041 tccaatcgaa gaaaacaagc gatactctgc taagaaaaga caaagaagtg tacctaaaag
     8101 attgactaga gaatgttcaa ataaagcaga tagaagaaaa ctgattgtac ttaaaaagag
     8161 cacccacaac aacaggaaca gaaagctctg gaaaagatac tcatacatcg gcaggattcg
     8221 tatctcctga ccaatgatcg ttcctacagg ataacggaag tctcccaaac catttttgat
     8281 tattactagt atagagacga tcaaaaatgg catcacgaga cttagcatag ctgcaccata
     8341 ataaatggta aaacggatgc tatgtttctt taccatacta tccggtaata gattgactag
     8401 agagattacc tgctttcttt tttcataaga gatgatggaa gaaagaaaca aggcggttat
     8461 acataaaatc aaaaaactag aaatcattcc tgaaaaaata ttttcgtagt aatttgctaa
     8521 agagagatgc gctggcaagt caaaaataga atatctttgg atctgatgtt ctttcatata
     8581 acggagcaac tcgacacgtt tttgttgttc aatgattgga attccttgtc tttgaccaga
     8641 aatcagtcga tccagtatat ctttttcata atgataagtt gcttcgacca tttgctggac
     8701 attccctgtg ttttttgcat gtaagattgc ttcgatcagt ttgttgcttt tctctaaact
     8761 ttccacaaat tctttctcat tctcatcttc tttcaacgtt tcaatcaaac gttgcatctc
     8821 ttcatagtta tgattcagtt catcgaattc tgtataagca agtttatgat caaaaaaaag
     8881 tgggaatgca aagagcacga ccaacaatag aggaaataaa aagtttactt tgttttttag
     8941 taagagattg cgttcaaatg taagtaaaga catagttaca cctcatataa tttacgataa
     9001 atagctaaaa tctcttccat atttgaggat tctgtgacga tttctccatt ttttagaaaa
     9061 catgctttag aacaaatcga ttggatattc gcgatagcgt gggaagaaag aagaatcgtc
     9121 ttcccctctt tctgccattg tctgatgagg ctattcaaga tatcaatact tcctgggtct
     9181 aatccgttca acggttcatc gaataacatg acagatgaat cactgatcaa gtaagcagca
     9241 agcaagacat gttgtttcat tcctagagac atttttccaa tcggcaaagt aaggtactca
     9301 gtcatcttca atcgttggaa aacagtttgg cattctactt tgctctgcca atagtatttg
     9361 atcaaagtca aataatcaac agccgataga tagtcggata actgattcgt tgattctaga
     9421 aaaaagaaag accggcgata atctgtataa gcagtaggag agatgttgtg aagggacaat
     9481 tgatcataag ttgttggcag cagttgggca atgccattca gcaaagttgt tttgccaaat
     9541 ccgttcgggg caatcaaagc agctacttct cctgattcta ctaaaagatt caattgtcga
     9601 atcacacgtt ttttaccacg tttcatgttc atgtttttga tcagtaatgt catagatagg
     9661 cctccaatat aaaatttaat aacataatag aatggaaaac atctaaatta aatgctaaca
     9721 aaagttattg tttgcatatt ataaaaaaat taaaataaca gttttattta aaattgaagt
     9781 tttctgatac cgaaatcttt ttattttgct gacatactca aacctttggc acaaagtttc
     9841 gtgccaaagt acgactattt ctctttttag taagcggata caattagagt atcaaaagga
     9901 gagagggaga gacaaaatgg aacggacgat tcccaaaaac aacatttcgc taaaagcgag
     9961 ggtacaaaaa ttaggcagta ctttatcaag catggtcatg ccaaatattg gagcactgat
    10021 tgcctggggt gtattaacgg ctctatttat cccagatggt tatttaccaa atgaggcttt
    10081 cgctaccatg gtaagcccaa tgctaactta tctgattcct ttattgatcg gttatactgg
    10141 cggaaaagta attgctggtg accgcggatc agttgttggg gcaattgcaa cgatgggtgt
    10201 gattgtcgga acagatattc caatgatgct aggcgcgatg atcatgggac ctcttggtgg
    10261 atatgccatc aagaaatttg atcagctatt ccaaaaacgg atcaaatccg gttttgaaat
    10321 gctagtcaat aatttttcag caggtcttat cggttttgga ttagcgttat taggtttttc
    10381 tgcgattggt ccagttgtcg atgcattgac tcaagcaatg gcaaaaggtg tagagatcat
    10441 tttatctgcc catttgattc cattgacaag tatttttatt gaaccagcaa aaatcttatt
    10501 tttgaataat gcaatcaatc atgggatttt gacaccattg ggaactgaac aagtagcaga
    10561 ggctggaaaa tctattctat tcttgcttga agcaaatcct ggtccaggac ttggcgtatt
    10621 acttgctttt actttgtttg gcaaaggagc agcaaaatct tcagcaccag gagccatgat
    10681 cattcacttt ttaggcggga tccatgaaat ctattttcct tacgttatga tgaaaccgtt
    10741 attattctta tcagttatag gcggaggaat gtcaggaagt ttcattttcc aattgatgga
    10801 tgctggacta agagcgccag cttctccagg gtcgatcatt gcgattctag cgatgacacc
    10861 gttcaattct taccttcctg ttattttggg cgtgattgtt gccgcagctg tttcgtttgg
    10921 tatttctgca gtaattttga aagcggatgc gaaagataca aatgaagact tcgaaaaatc
    10981 tgttcaagag acaaaggcag ctaagcaagc ttccaaaggt gcccaaggta ttcctcaagg
    11041 agctacattc tctggcgtac aaaaaatcat ttttgcttgt gatgcaggaa tgggatcaag
    11101 tgcgatgggc gcttcgatct tgagaaaaaa agtacaggaa gcagacttgc ctcaaacggt
    11161 gaccaatcaa gcaatcagcc aactgacaga tgactcgaat acattgatcg ttactcaggt
    11221 cgaactacaa gaaagagcaa aacaaaaagc accgagcgcg cgttttgtct cagtagaaaa
    11281 ctttttgaat tcacctcgat atgatgaaat cattcaagaa ttaagcagca agcagaaaac
    11341 atcagtttct gaaattgaga atgagccagc agaagagttt ctttcagcaa acatcaaaca
    11401 aatcaaagag attctacttg tccacgatga tcgttccgga tcagcaacaa tggggatgac
    11461 cgtgctcaaa gatatattag acaagaacca tttaactatc ccgttaagaa aagtcaatat
    11521 ccaagattta aaagatgaac ctgattcact gatcgtagcc aaagaaagtt tagcggaaaa
    11581 agtacagatg gctgcacctc aatccattca tttatcagtc aacagcatgg tcaatccgca
    11641 aaaatacgaa tcgattgcac aatatttgaa acagacagct taagggagga aaacagcatg
    11701 caaagtttgc cattcgacat gatcgagctc aatcgatctt ttaaaaccaa agaagaagcg
    11761 atccgctatt gcggtcagat gcttgtagaa gcagggtgcg ttgatgagga atatatcgat
    11821 gccatggtag atagagacaa aatgttatct gtttatatgg ggaattttat cgcgattcct
    11881 catggaacag atgaagcaaa aaaatatgtg aaaaaatccg gcatctgtgt cgttcaagta
    11941 ccagatggtg tagattttgg taccgaagaa gaagaaaaga ttgcgactgt tttgttcggt
    12001 attgcaggag tcggagatga gcatttgcaa cttgtacagc aaatcgcact ttattgcagt
    12061 gatatggaca atgttgtcca gctagcagac gcgctcagca aagaagaagt aacggaaaat
    12121 ctagcaattg cttaaggaga gagaaaaatg agagcagtac attttggtgc aggaaatatc
    12181 ggtcgaggat ttattggaga ggttttggca gctaatggat ttgagattac tttcgtagat
    12241 gtgaatcaca cgatcattga tgctttaaat gaaagaaaag aatacgaaat cgaattagca
    12301 gatgaatcaa aagaaaaaat cactgtaaaa aatgtcaaag gaatcaacaa tcaaacaaat
    12361 ccagaaaaag taatccaaga aatcgctgaa gcagatttag ttactaccgc cattggcccg
    12421 aatatcttac cttttattgc tgagttgatt gcaaaaggta tccaagaaag agaaaaggaa
    12481 ggaagcacaa catctttaga tatcattgcc tgtgaaaata tgattggcgg ttcagcattt
    12541 ctagaaaaag aagtgtataa atatcttccg gaaacatcat ttactgacaa atatatcggc
    12601 tttccaaatg cagcagtcga tcgaatcgtc ccattacaac accatgaaga tcctttattc
    12661 gtccaagtgg aacctttcaa agaatgggtc attgacgata gccaaagaaa aaataaaaaa
    12721 atccaattaa aaggggtttt atatgtagat gatctagaac catatatcga acgcaaacta
    12781 tttagtgtca atactggtca tgcgactgtc gcttatacag gtgccttgtt aggttaccag
    12841 accattgatg aagcaatgca agatgcgtta gttgtggcac aattaaaatc agttttgcag
    12901 gaaacaggaa gtttgctgat tgccaaatgg gggtttgatg cagagcagca tcatgcgtat
    12961 attgaaaaaa tcattcatcg cttccaaaac aaaaatatct ctgatgcgat cacacgtgtc
    13021 gctcggacac cattacgtaa gttaggttat caagaaagat ttacacgtcc agtgagagaa
    13081 ctacaagaac ataacttgac ttgcccacat ttgacggcaa caatggggat tatttttaat
    13141 tactatgatc ctgaagatga acaaagccgt caattgcatg aaatgaagat ccatgaaaac
    13201 ttggaacaat tgattcaaga agtcacagga atcaatgatc cgaaaacaat cgggaatatc
    13261 aagcaaaacg tgaaccgtta tgccaagcaa gtcgcttaaa ataggatacg aaaaagggga
    13321 atgtgacgaa atttttgtca cattcccctt tttcgaataa ctaggctgtt tgtccttcaa
    13381 gatagggcag atccaattct ttaccgtaac gttgttctaa agctaggcgg atcaaacgag
    13441 ctgccaggtt ctcatttctt gcaaggatag gtccatggaa ataagaaccg aagacatttc
    13501 ggtaaatgac accttcagat tggtcttcac cattatttcc ttgaccttgt accactttcc
    13561 ccaaaggtcg ttcaccttca cctaagaaag tccgaccatt gtggttttca aatccataat
    13621 aagtttcatt gaattcttcg ttatggataa cgatatcacc gataaaccgg ttattttcct
    13681 gacttaacgt gtagtgatcc agtgcactga ttccttggat cttttctcca ttagctccca
    13741 tgtagtagtg acctagcaat tggtagccac cacaaatggc taacatgacg ccatcatttt
    13801 cgatatagtt gatcaaagaa tctttctttt tctggatgtc ttttgaaatg attaattgct
    13861 caaagtcttg accaccgcca aagaaaacca agtcatattt atcagcatca aaaggttcat
    13921 gtatactaac gatctccgta tggcaagaaa cccccatttt ttctgctata tatttcaaca
    13981 tcaaaaggtt tccgttatca ccatatgtgt tcatcaaatt tccataaagg tgggcgatat
    14041 tgagttcata attagccacg gttcattcct cctttgataa atccttgagc agctagttct
    14101 ttacgaagct gcaatacagc agtgtaagta gctaaaatat agacatgctc agttggcaat
    14161 tctttgattt tttcgattac ttctggtaat tcttttgttt ctgtcaattt attttctgga
    14221 atcccggcaa cttttagacg taaagtcata tctgtatgac ggtctcctcc agaaataaca
    14281 gctgggatat ccatatcggc aaagttttca aaattaccat cccaaatcca gctgacatcg
    14341 attccgtctg cgtaattggc atttaataaa gccactaaag aaaatggata aggagctaga
    14401 ccaatcatat cgattacttg attgatacct acaggatttt tgacaagtac taatgtacat
    14461 tttttcccat tgatattgat gacttcttgt cggccgaata ctttctcgtc atatcctaaa
    14521 ccttgtttga ttttatcagg atcgacattg aagtactctg ccactgcagt agctgctaaa
    14581 gcattgtaga cattatacat accgccaact tcgattccgt attcatgacc atcgatcacg
    14641 aaatctgctg acacattgtc catccgtacc atatcagtta atcgataatc caattcagga
    14701 cgtttgaagt cacaatttgg acagtaatat ttccctaagt ttgcataagt gatcatttta
    14761 taatgcaaga tgtgatgaca atttggacat aagacaccgt ctgtattgta atgagccatt
    14821 tgttcacgat cttcttcatg gtcaaagcca taatattttc taggattgac tgtttcgata
    14881 gaattgaaga ttggtgaatc accgttactg ataatcgttg catttggtga ctttgcggca
    14941 ccgtctacaa tcattttata ggtcgtatag atttctccat accgatccat ttgatcacgg
    15001 aaaatatttg taaataagaa cagttctggt ttcatatatt ctgtgacttt gctcaaactt
    15061 gcttcatcta tttctaggac agcaaacttt ttctgtcctt tttttggttt tgcctgcaag
    15121 aaagtcgata caattccctg taccatattc gcaccagtag ggttggttag aacttcatcg
    15181 aattgctggc gcaaaatatt gactgttaaa gcggtagtca atgttttacc gtttgttccg
    15241 gtgacaacga cgacttggta gtcttttgcc aaactatcta gtatatgtgg atcgactttc
    15301 agcgctagtt tacctggtaa acttgatccg cctttaaaaa aagtttttaa tgcccattgc
    15361 gacgtttttc caactgcaat cgcaaaatga ctgcgtattc ccattatgta acctccaaaa
    15421 acgatttgct gacgaataaa ttcaactaat cataccatat gttgaaaaaa aggttaaatt
    15481 gcttttctta ttcttaagaa aaggttacgg ctgaaagtgc atgaaatcgg acaaaatcgc
    15541 atgatttaac aaaaaaacag cttgaaacaa aatgattgtt tcaagctgct gtgattttat
    15601 tattccctct tacctatatt cccattttaa ccgataataa ttttctcgct tggatattca
    15661 tagcctagat ctcgtgtttc ttttggcacg aataacatca atgtatatag cattccgatc
    15721 cgcccaataa acatcagtgt agcaatcgtg attttgccca caacagacaa gtctccggta
    15781 atccctaaag ataaaccagt tgttccaaac gcagaagtta cttcgatgat gatcgagatc
    15841 agcgtttgtt cttctgtagc agataaaaaa acgatacaaa agaaacacat accaagagaa
    15901 agcataaaga caacgactga ttttcgcaca tcatcttgat cgattctccg tccaaaaata
    15961 ttgatgttat cttcactttt gaggaaggaa tatagataca aaccgatgat tgctacagta
    16021 gttgttcgaa tcccgccgcc tacagaacta ggactgcatc cgataaacat cagtaaagaa
    16081 aaaatgatca gtgttgtgat ttgaaaatca cccaaatcgt gaatctgcag accagcatta
    16141 cgagtcgtaa tcgaataaaa catcgaattg atccatttga cactcatatt ggaatcttgg
    16201 aataaatggt ccttttccaa tagataaatc aagacagttc ccgaaacaaa caagataaca
    16261 aaagctaaca cagctaattt ggtaaacaag gaaaaacgaa acggaagctt cgcattggaa
    16321 cgtttgtata atagccattc ccggcactcc atcaatacag gaaaaccaat tcctccgaca
    16381 aaaatcaaaa acatgatcag gatcaagaaa aaatagtcat gtgcaaaggg cttgatggaa
    16441 tccccagtga cgtcaaaccc agagttcgtg acagccgata tcgcctgata aaaaccgtaa
    16501 aaaacggcat cacgccaacg atcaaaatag cctctgtaat aaaaataaat agaaaagaat
    16561 gtcccaaaaa gtaattgaaa ccagattaaa atagcaaaag tgatccgaat cagtcggacg
    16621 ataccagaca aacgcggttg attcatatcc gtcatgatca gctgtcgctg tttcagtgtg
    16681 atccgtcgtt tagaaaaaat gacaaaagca gtagaaatca tcatgatccc caaaccacca
    16741 acttgaaata atatttccag cagtataata ccgttatcat tgaacacaga attgatatca
    16801 aaagtcgata gtccagtcac actcactgta ctgattgcca tgaacagcat gtccacaaaa
    16861 gaaacatggc tatctggttc gcgaaaaatc ggcatataaa atagaacgag tgataagacc
    16921 gtcatcaata tataataaaa aacgatgatt tggatcgatg agaaatgatt ggcagcaaac
    16981 ttctgtcctc gccgctgcag ccttcgaaag aataaatcaa catgcacagt actatcctcc
    17041 tgacaatagt cgtttctacc agtatacaag gggaattagg aatactcaaa gaaattatca
    17101 aacagataac aatacagctt tcgattaaaa tagaaatggc ttataataag gaaaatccta
    17161 gaaaaaagaa ggaggaggaa taacaaattg attaatgctc aacaaatcgt agaaaaaatg
    17221 aaaaatcaaa aaataaatta tgatacagtt ctgaaaaaaa tgatcatgca atgggagaaa
    17281 gaagaagtaa agcctaagat attaatacat acatgctgtg ctccttgcag tacgcacaca
    17341 ttggaattca tgtctcaata tgcagagatc acgttatttt tttcaaattc gaatattcat
    17401 cctaaaagtg agtatttgcg aagattacat gaacaaaaac gatttgtaga ccaatttaat
    17461 gagaaaactg accatcatgt tgcgctaatc gttgatgaat accgtccaaa tgaattcacc
    17521 aaaatggttt taacaaacca tcttgaaaac gaaaaagaag gtggtgctcg ctgcagtgca
    17581 tgcttcaata tgcgattaga tcttgttgcg caaaaagcgc aagaactagg atacgattac
    17641 tttggaagtg ccctgaccat ctcgccaaag aaaaatgcag ctttgatcaa tcagatcgga
    17701 atggatatcc aaaagattta cgggacacaa tacttgccaa gtgatttcaa gaaaaacaaa
    17761 ggctatgaac gctctttgca aatgtgcaaa gagtatgata tttatcgcca atgttattgt
    17821 ggttgcgtgt tcgctgccaa gcagcaagga tgcgatctga aagaaatcaa tcgggaagca
    17881 aaagcttact taaaaacatc aaccgtagtt tttgaggaaa attaaagaaa tctttcggaa
    17941 aaacgtaaga aaaaaataaa gaatttatgg tagaaataag gtagactgta taatttgtcc
    18001 gtagtaaagt aaggaaacaa gaataaattt caatgaagga agagataaga tgcgaaaagt
    18061 agcacctatt ttgaagccaa tcgctgtatt aacaatctcg acgattgtcg cgactactac
    18121 aggtattgtg aatagttcca gctaccgtgc accaagttca agttctagta ctgtagaaga
    18181 aacaacacca actacttcag tctcgacatc tgaaccaaca acaacggaaa gtttgaatga
    18241 aaccactacg acaagtacat gggacgatac aacaactagt tcgtcgtatg atgagacata
    18301 tacaactgac agctcaagcg aaacaagtaa tacggtacca gatggcactc catcggattc
    18361 aaccaatcca agtacaagtc cggatacaag tgctagctcc gaaacgatac caagcagtac
    18421 gactgtagat gataacacgc aaaatgatga tgcagcacgt acagcacagc aaaaccaaga
    18481 ttcaggaaat acccaaacgt ataaccagca agcaggtgat tctcaatgac atttttacta
    18541 gaccttatca acggggtgca taaattcctt ggctatttag acatcagtcc taaatatctt
    18601 aaccgtggat atacgatttt aagtgtgatt cccacgcttt atttattgag aattgtttat
    18661 ggactttggc aaaaccaaaa ctatcttcaa tttttcttat atggacttgc atttctagtg
    18721 cttgtttatt ttactgtttt aaatgtattt tattactttt tagataaaaa cacgaaagcc
    18781 gatgtcactc agctttttgt gaagtattta cctgatgaag cgttcaacat ccagacagaa
    18841 gaatcgatag ataatggaaa ttcgatcgat aaagtgaata cacaagaggt catggtttct
    18901 tatgacgaag attatcaatt gaaattagcc gagaatatgc ggtatttgat tggaaatggt
    18961 gagatcaaaa caaatgaact cggtcgcatg gatggatttc tggtcgatag aaatacattg
    19021 tatccttatt actatgtcaa gaaaacagga gagaaagagt atgttctaaa tattggacga
    19081 agctactcgg acatggaaaa agtagggacc attcgacttg actcaacaga agacacattg
    19141 gaacctgtcg gattgttcat cgtcggcggt gattttgtca aagacgggtt acgctatcat
    19201 gagccttatc gtctgaaatt gattgcaaaa aaggcacagt cgcaggaaga accagtggtt
    19261 tatagtcgta gccaaagaag aagacattca taatgtgatg aaacgccgtg tatcagtggt
    19321 tgcgataaaa acgtttattc ggaataaggg ggatgtgaca cgtcttttgt cacgttcctt
    19381 ttttatataa cgagaaagat aagacgaaca catcacttat tctttccttt catttataaa
    19441 cattgtagaa taaagaaagt acaacgaagt attgtgaaaa aacgtaataa ataacgagga
    19501 aaatacaagt aggaggtaag ctgtgaaaga atttttgaaa aattggggga ttttgatcct
    19561 tgtattagcg gctattttat tagctagagt gtacgtgttt acaccagtta ctgtaaacgg
    19621 gcattcgatg gaccctacat taagtgatgg acaacgattg atttcatcta agatctcaaa
    19681 ctatgaacga atggatatta ttactacaaa ggaaccgggg gatgaagaac ggatgattgt
    19741 taaacggatc atcggtatgc ctggagatac agtcaagatg gaaaatgacc agttgaccat
    19801 taatggcaaa aagtatgatg aaccttatct ggatgagttc aaaaaagaat tttcagaaga
    19861 taagttacaa ggagaatatg cttatagctc aggattccaa gcacaagcag aaagttcatc
    19921 cacctttacg agtgactttg aatatactgt gccaaaagga aagtatcttg ttctaggcga
    19981 taaccgcttg atttcaaaag acagccgaat gtttgggtta gtcgacaaag atatgatcca
    20041 aggaaaagtt gttttccgtt attggcctct ttcagaaatt aaaatcatgt gatgaaaaaa
    20101 taaaaagaag ctgtgacatg ttttttgtca cagcttcttt ttccgaaaat tccttcttat
    20161 ttcttggagt tgctcacttt ttttgcaatc tcattttaag ctttttggct gatggataag
    20221 aaaagcgaag cgtttttgtt cataaatagc ttttccccac gatggaaggt atgactagta
    20281 gcagagacaa agcccgtttt ttccaatcga ttattcaatt cttcttggct aaagccatta
    20341 tggactttcg gatgactaat ttgttcattc ttgtcaaagt ctacgatgat caattgaccg
    20401 ccaggagcta aaatttcata aagttttgta agaatattct ccgtatcagg aatgtgtaaa
    20461 agtacaagtg atagaaggat gagattagct tggacagcag gaaattcgac agaagcatca
    20521 gcgtggatcg ttcggacatt tttcagatct gctgcttgga tcttttcttc tgccattttc
    20581 agcatagttt ctgaagcatc ggcaatgatc agttctttga atctttccgc aagcggtaaa
    20641 ctaacaagcc cagttccgcc tccgtaatcc agcaggactt tttggtggct gtcgcgcggt
    20701 agataagtag ttagttcttc gcgaataatc ttggctaatg cttgacggtc aggggaatca
    20761 taatggttag cgatggaatc aaaaatattt tttgtcatca aatagctcct tacgtaaaac
    20821 tttgaaattt taacttatta tagcacgtga aaagaaagaa agcatgctat actcaattca
    20881 aaaagggaga atctagaaag aatatgccga tatcgtttgt aaaagataga gaggaaaaag
    20941 gaaagtgtgt ccgagaaata ttactagact tgcctgagtg gtttgggctt cctgaatcaa
    21001 cggaaaaata tatcgaggaa tcaagtaaac tacctttatg gtgtgaaaaa agaaaagaag
    21061 aatatttagg ctttatcact ctttcacaga caagtgaaga tactgccgaa atctattcta
    21121 ttgtatggga ataaaaaaag actatcatca ccaaggatta ggaacaaaac tttttaaaga
    21181 agcagaaggt tacgcagcaa aacattacaa atatttgcaa gtgaagactg tagacgaggg
    21241 gcactactct atatatgatc aaactatttg tttctacgaa tcattaggat tttcaaggtt
    21301 ggaagttttt ccaaatttat gggatgaatg gaacccatgt ttagttcttg tcaaaaaatt
    21361 ggaacaaaag tgagcaatag atttaatact aatctgtgct tgatgaaaat tctcactgta
    21421 aaaaatgctg aaaatcaagt ataataccat aggaacaaac tggaaggtga aaaagagcat
    21481 ggcacaacta ttttttaagt atggcgcaat gaatagcggc aagacgatcg agatcctaaa
    21541 agtcgcacat aattatgaag aacaaaataa gccagttgtc ttaatgacaa gcggtattga
    21601 tacaagagat ggtttaggcg ttgtttcaag ccgaatcgga ctgaaacgta atgcgatgcc
    21661 gatatttgaa gaaacagacg tatttgacgt aattcagaaa atggaaagca aaccctattg
    21721 tgtgctgatc gatgaagcgc aattcttaaa aaagaaacat gtcattgctt ttacgagaat
    21781 tgtggacgaa ttggatattc cggttatggc atttggtttg aaaaatgatt ttcgaaacga
    21841 gttatttgaa ggatcaaaat atttattgct ttatgcagac aaaatcgaag aaatgaaaac
    21901 aatctgttgg ttctgccaca aaaaagcaat catgaattta cattatattg atggaaaccc
    21961 tgtttatgaa ggcgatcaag tgcaaattgg cggaaatgaa gcttattacc ccgtttgtcg
    22021 aaaacattac tttcacccat caatgattac agaaggagaa taagcatgta cgatcaatta
    22081 caagcaattg aagaccgtta tgaagagctt ggagaacttt taagtgaccc agaagtgatt
    22141 tctgatacaa agcgttttat gcagctttca aaagaagaag caaacacgcg cgaaacagtc
    22201 gaagtttatc gtcgttataa agaagtagta caaggaatca ccgatacaga agaactgctt
    22261 ggtgaaaaac tggatgatga aatggctgaa ctagcaaaag aagaattatc tgaattaaaa
    22321 aaagaaaaag aagtcttaga agaaaaaatc aagatcttgc ttttaccaaa agatccaaat
    22381 gacgataaaa atatcatcat ggaaatccgt ggagcagcag gcggagacga agcagcatta
    22441 ttcgctggcg atcttttcaa tatgtaccaa aagtatgctg aagcacaagg ctggaaaaca
    22501 gaagtgatgg aagctaacgt taccggaatc ggtggatata aagaagtgat catgatgatc
    22561 accggagaca acgtatattc gaaattaaaa tatgaaagtg gtgcgcaccg cgtccagcgt
    22621 gttccttcaa ctgaatcgca agggcgtatc catacttcca ccgcaacagt tgtcgtgatg
    22681 ccagaagctg aagaagtaga gatcgatatt gaagacaaag atatccgtac agatatctat
    22741 catgcttcag gtgctggcgg acagcacgtc aataaaacag catctgctgt acgtttgact
    22801 cacttaccaa cagggatcgt tgttgctatg caagatgaac gttctcaaat caagaaccgt
    22861 gaaaaagcaa tgaaaatctt gcgtgctcgt gtgtacgata agatccagca agaagcacaa
    22921 agcgagtatg atgccaatcg aaaatcagca gtcggaactg gggatcgttc agaacggatc
    22981 cggacgtata atttcccgca aaatcgtgtg actgatcatc gtatcggatt aactatccaa
    23041 aaattagatc agattttagc agggaaatta gatgagatcg ttgatgcatt gatcatgtat
    23101 gatcaaacat caaaactgga agctatgcaa aatgggtaaa atgacctatc gagaagtcct
    23161 gtcacgggct tcttcttttt tagaggatca tgggaaagaa gcatatagta tccaattttt
    23221 atttttggaa cgaaaacaat ggaaaaaact tgattggctc cttcatatga acgaagagat
    23281 ttcagaagaa gaacagcggt tgatcgaaac cgatctgcga cttctgcttg cagatcatcc
    23341 acctcagtat ttgctaggat atgcagattt ttatgatcat cggttaaagg taactgaagc
    23401 aacactgatc cctcgaccag aaacagagga gttagtcgaa tggtgtttag acgagacgcc
    23461 tggtgtaccg ttagaagtga tagatattgg gacagggact ggagcaattg ctattagttt
    23521 gaaagcagca agaaaaaatt ggcatatatc tgctgtggat ctttcagaag aagcattaga
    23581 agtagcgaaa gaaaatgctc agaaagaagg aacgaaaatc agtttttatc atggagatac
    23641 attaggacca gtaatggatc agcaatttga tgtgattatt tctaatccgc cgtacatcag
    23701 tcggaatgaa tgggaactga tggatgaaag tgtccggtca ttcgaaccta aaatggccct
    23761 ttttgctgag aacgatggat tagctattta tgaaaaaata gcagttgaag caccaagcgt
    23821 actaaaatca aatggaaaga tttttttaga aatcggattt cgccaaggtg aagcagtaaa
    23881 aaaaatattt cagcaagcat ttccagataa aaaagtggcg ataaaaaagg atctttttgg
    23941 caatgaacga atgatccaag tttatggttg aagttgtgga tgaaaaattt tttatttgtt
    24001 tttttattat aaaaaaacac ttatttgaca aggatgtaaa cgactttttg gtttatcaac
    24061 aagtttatcc actattgaca aatagattat ccacaaacag tggaaaactt gaggataaac
    24121 tcagacaaaa taagaatgta tgaaaaaggg gttgtggata aactgtggaa acgaaacgat
    24181 atgatgaaac agaattgcaa gaggcagccg aagcactgaa agaaggagaa cttgttgctt
    24241 ttcctaccga aacagtatac ggattgggcg ccaacgctct tttaccaaac gctgtcaaga
    24301 aagtgttttc tgtaaaaggg agaccgcaag ataatccatt aatcgtccat gtcgcctctt
    24361 ttgaacaagt aaaagagtat gtggacaact ttcatccggc agcagagaag attgtgaaga
    24421 acttctggcc tggaccatta actttgatat ttaagatcaa aaaagacact ttgccctcag
    24481 ttgtgacggg aggattatcc acagctgcat ttcgtatacc ggataacaaa aaaacactag
    24541 aagtaatcga actttccggt gttccgcttg tcggtccaag tgcaaatacg tcgggaaaac
    24601 caagtccaac gactgcagat catgtatatc atgatctaca aggaaaaatt accgggatta
    24661 ttgatgatgg agctacgcga attggtgtag aatccactgt tcttgattta agtgatccaa
    24721 cagccatgcc tatgatttta cgaccaggtg cagtaacgaa agaacaaatc gaagcagtca
    24781 tcgaatctcc tgttgctatt gatcagcatc ttgtaaaaga aaatgaaaca ccaaaagccc
    24841 ctgggatgaa atacaaacac tactcacccg atacacgtgt tctgatggtc agagatggcg
    24901 attggtcaac tgcagttcaa tgggcaaaaa ataagaagat ccgtgcaggt gtcatagcaa
    24961 gccccgaaat tgcggatcaa gtgcggacag atactgcagc tgtttatatg tataacgaca
    25021 actcagtaga agcggcggct aaaggattgt ttgcaggtct aagaggatta gatgaaccaa
    25081 cacttggatt agatttgatt tttgtacaag tgtatccaga aactggattg gggaatgctt
    25141 acatgaatcg cttgaaaaaa gcagcaggac aaaattattt tgaaaaatga tcaattggtt
    25201 attgtcgcat tcttttagta tgatacaatg ctatcaagta aatcttctat cagtgaacga
    25261 aaaactaagc gtaactaatc gtaatagaag agatacgaat atttggcaat ttgtttttga
    25321 tgggaaaaat aaaattgtag gaaaataaaa aggagctgtt gtttgtggta gattacaaaa
    25381 cgtttgaccc tgatctttgg gctgcgatcg ccaaagaaga agaaagacaa gaacataatt
    25441 tggagctgat tgcctctgaa aactttgtct ccgaagcagt gatggctgca caaggaagta
    25501 tcttgacaaa taaatacgca gaaggttatc cagggcatcg ctattatggt gggtgtgaat
    25561 ttgtcgatat cgttgaaaat ttagcgattg accgtgcaaa agaattattc ggagcgaaat
    25621 ttgcgaacgt ccagcctcac tccggctctc aagccaacac ggctgcttat ctcgctttgg
    25681 tagaaccagg ggacacgatc ttaggaatgg atctttccgc aggcggacat ctaacacatg
    25741 gatcaccagt caacttcagt ggcaaaacct atcactttgt tgcttacggt gttgatccga
    25801 caacagaagt tattgattat aatgtggtac gtattttagc tagaaaacat caaccaaaat
    25861 tgatcgtagc aggtgccagt gcttacggac gaacgatcga ctttgcgaaa ttcagagaaa
    25921 tcgcagatga agtaggcgca aaactgatgg tagatatggc acatatcgca ggattagtag
    25981 cagcaggact tcatcctaat ccagttcctt atgcagatat cacaacgact accactcata
    26041 aaacattgcg tggccctcga ggcggcatga ttttgacaaa cgatgaagca ttagcaaaaa
    26101 aaataaatag tgcggtattt cctggtattc aaggcggccc tctagagcat gtgattgcgg
    26161 gtaaagcagt agcattcaaa gaagcattag atcctgcatt taaagaatat agtgaacaaa
    26221 tcatcgccaa tgcaaaagca atggtcaaag ttttcaatca agcaattgga accagagtca
    26281 tttcaggggc gacagataat catctgatgt tgatcgatgt tcgcgagtta ggtataaatg
    26341 gaaaagaagc agagagcatt ttggacagtg tgaatatcac tgtaaataaa aactcgattc
    26401 cttttgaaac actaagtcca ttcaaaacaa gcggtattcg aatcggcacg ccagcaatca
    26461 ctactcgcgg gttcaaagaa gaagatgctg taaaagtggc tgaactagtt gtcaaagcat
    26521 tacaagcaaa agatgataac gctcaactag acgaagtcaa aacaggtgta cgcgaattaa
    26581 ctgaaaaatt tccgttgcat aaaaaataaa aatttaaatt ccattagtaa acagaaaagt
    26641 tcaatgttgg gcttttctgt ttattttttg ggaaaacgga ttctttgaca atatcggtga
    26701 tgctctctag tatatgaggt gtaattttag tcttcacact aaatctcagg gaggaaaagt
    26761 catgactgat cgtatctcgt tagaaaagga agcagtagca ttcagtgatg caaatgcgcc
    26821 tcatccgcgt atctatgaac tagcaccgga agaaggaaga gttttactag aagaagtaca
    26881 agaatcacct gtgaacaaat attccgtcga tattgaagac ttgatggtgg atactggcga
    26941 atggggaaaa ctcaatattc gtctcgtacg accagaaggg aatatggata aattaccagt
    27001 tattttttat attcatggag caggctgggt ttttggcagt gctcaaaccc atgataaatt
    27061 gatcagagaa cttgcggttc gtacgaattc cgtcgttgtc tttccagaat atagtcgatc
    27121 accagaagca aaatatccaa cagcaattga acaaaattat gctgtgttgc aacagctgaa
    27181 taaattagcc aagtcaaaaa atctggatct cgagaatcta atcgttgcag gagattcagt
    27241 aggtggtaac atggctacag tcatgacgat cttaacaaaa gaacgaaagg gcctcccaat
    27301 atccaagcaa ttgctgtatt atccagtcac aaacgctgat tttgatacag attcctatca
    27361 tgaattcgga gaaaactatt ttttgacaag agaaggaatg atgtggtttt gggaccaata
    27421 tacgacgaat gaagaagagc gtgctgaaat cacagcttcg cctttgagag caaatataaa
    27481 tgaactaaat gatcttcctt cagcaatgat tttaacggga gaagcggatg tattaagaga
    27541 tgaaggtgag gcttatgcgc gcaaacttcg tgatgcaggg gttcctgtta cacaagtacg
    27601 cttccaagga atcatccatg attttgtcat ggtaaatgct atggatcaga caaaggcaac
    27661 gcgtgcagca atggcattat ctacttcttg gatcaagaaa aaagaataag ctttgcttgt
    27721 ccgcaggaaa acttcttttc aaaaacggat tttcttagga aaaatggcga gtttgactag
    27781 tcaatcactt gattttgttt tacaattaaa aagaaacgac agaacaaagg agagcttaaa
    27841 aaatgggcaa atttcaagtg atcgatcatc cattgattca acacaaacta actattattc
    27901 gggacaaaaa ctgcggaaca aaggtttttc gtgaagtagt cgatgaaatc gctatgttga
    27961 tggcatatga agtatcacgg gatatgccat tggaagacat cgtgatcgaa acaccgatca
    28021 ctgaaacaac acaaaaaacg ttgtctggta aaaaagtagc gattattcca atcttgcgcg
    28081 caggtattgg aatggttgac ggtatcttgg aattgattcc agctgctaaa gtgggacata
    28141 tcggtcttta tcgagatgaa gaaacattgg aaccacatga atatttcgtc aaacttccag
    28201 aagacattga tgcacgtcag ttgtttgtag tagatccaat gttggcaaca ggcggatcag
    28261 caatcatggc aatcgatgca ttgaaagaac gtggcgcgtc gaacattaaa tttgtttgtt
    28321 tggtagcagc acctgaggga gtaaaagctt tgcaagaagc gcatcctgat attgacatct
    28381 atacagcttc tctagatgaa aaattgaatg aacatggtta tatcgttcct ggactagggg
    28441 atgcgggtga ccgtttgttt ggtacaaaat aagtaaatac gaaaaagggt gtgacacaac
    28501 ttttgtcaca tccttttttg gtcattttta acgtttgatg cagaacagct ttttcacagc
    28561 tttcgtttgc atataaaaaa accatcccaa aaatgcagag atggtcaaat tcaaatcttt
    28621 tttagttctt tgtagttaac gattactaca cccttacttt gcgtcatcaa gttcgcattt
    28681 tctgcagttt catcaatctc aacaacagcg gaatttttca attgctttga aatcgttcca
    28741 gtttgcgact tgccgtgaat tgtgaacatt acgtgagtat tgatcttgaa tttttcacca
    28801 atttccgatg gtacgatagt tgtatcaaat tttccaaaag ttgccatttt cataaaacct
    28861 gcctttcaca tcaattatac cacgttttat gaaaaatatc atgaaattaa actaattttc
    28921 catcggactc tttacaatca gccaaataga gtatataatt tcttttggat aaggccagct
    28981 tagctcagtt ggtagagcaa cgcactcgta acgcgtaggt cgtaggttcg atccctatag
    29041 ctggcattaa aagagaagac agatatatca aggattttta atccattgat acatctgtct
    29101 ttttgtttaa aggaaataaa gtatgctttc ttagcaatag agcatgtatt atttctataa
    29161 aaatttagga gaataaaacg actagcaata tagctgtgaa atcaaatatc acatgaactg
    29221 cccaacttgt aatgatcgag ttgcttttaa tggcagcttg gttgaataac aatcttgcca
    29281 ccccttgtat caaaaggatg tgtgctacag tgtgtaaaat atttccatta tcataagtac
    29341 tgtaatggat aaatccaaag atgattgctg acaagaaact agcgacagga atgctgaact
    29401 tactagaaaa aatagcgaga aaatacactg agaacaattc ttctccaagt atcatgatag
    29461 gcaaaataat aaataataac gggctgaaag cctcatttat tggattgcct gttaaacccc
    29521 atttcaaaat ttgttgcaaa aataccgaga ctgcggcact gattactaca ttgataaaaa
    29581 aatatttggc tatatttttg acaggagcga caggtttcga gaacattttt ttcgtatctt
    29641 ttttaccaaa aacaagtgta aatgctataa atccagtgat agttaaagaa aaaatgctaa
    29701 taatggatgg cgcaaatagc aaccctaaca aaccgagtac aacgacaata gtgcccaata
    29761 tccctttttt aagttctatt ttttttccta aaatgttatt catgataata actcctacta
    29821 ttcagttttt gaagatagag tcagtataca tgaagagtta aaataggcaa tgttttttta
    29881 tggatatttg tattgctaaa aatttatctt atatattcat tttgattata ctacttttat
    29941 ttgtaaaaat tttctagcaa aaccaagtgc ttgcaactga ttaagcgtac aattttttag
    30001 atgttccgag cgtacttcaa tcatttcaaa ggagttgcta cttaaatcga tattttttaa
    30061 atcagtatca aaaaagctag cattttctaa atcattagta gaaaacatta ttttagtgag
    30121 ggaacagtct acaaaatctg cgcttgataa gacagagtgc tcaaaggtac tttcttttat
    30181 gtttgaacca gataaattta catagtccat tttacaattc tcgaataacg tgttcccaat
    30241 ataactttcc atcaatttag tacctgttaa tttacaattg acaaatttca cgcgatgcaa
    30301 catgcttttt gtcaatatac agcctgaaaa atcacagttt tcgaatatac agtcggtaat
    30361 ctccattctt tgaaaatcat tggaagcaaa aaatgcatac ttgaaaatag atttgtaaat
    30421 aattggatgg cagattattt ggttttcgat gacctctttt tgtgaaagga tattatacag
    30481 ttcaaaatct tcgttttcat agatatgaaa aaaggatcct ttattcaagc ttgcttcatt
    30541 aactttagga aaaatcatat ttaattacct ccattttcat aaagtcatat taacaaaagc
    30601 taaaccaact tacattagca gaaaatgaga taggttttcg cctttttcga taacgatagt
    30661 gtcgctatct cttgaaacgt tcttagaatt gccctgagag acacaaatta ggcgtaattc
    30721 taataaaaat cttagaataa aaatttttac tgatttaatt tagaatgttt ttaaaataaa
    30781 aatcgttgcc ataacagtgt attgaaaggt tttaacaatc cagtaatgat ttaaaaaaat
    30841 ctagatgtag cgtatacttt gtttatagaa tgtagtggga agaaatatat ttttgggagg
    30901 aaatcgtgat gtctaaaagt gaagaaatag aaaacatggt actaaaatta tatgatctcg
    30961 ttttaaatcc agagatcaaa gaaaaggaac gtatactttt gatagacgca aagacaggct
    31021 tagaaaaagg tcagtattat cctaaagtca taaataattt ggaacgtagt ttacgtccgt
    31081 tagcaatcag gggagaattg tctaaaccag tatcaccgtt ttatatggag atatcaacaa
    31141 taggaaaatt tgaaaaagaa ttaggtcgag gaatggcttc ggcaccgatt acttttgggc
    31201 atttataaaa acgagttatt catctcaatg aattaaaaag tatagtcaat ctcataatgc
    31261 aggctaggtt catttatttg aacagagtct gtattttttt attctaaata aagaaacaga
    31321 t
//
LOCUS       DAZKLD010000015        30001 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.015, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000015 DAZKLD010000000
VERSION     DAZKLD010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30001
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.015"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            171..509
                     /locus_tag="ACG8KR_000608"
     CDS             171..509
                     /locus_tag="ACG8KR_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0200009.1"
                     /translation="MPDYKQKAGYSFCSDKFQLGKFTKLAGEEVSTVRIKECPLQIET
                     LVQRISTRQDFAIVEREIVAIFVEEGILHDPTHINVDKWQPLIYKFREYTTANQSLTL
                     NFRFQEFKES"
     gene            complement(714..1276)
                     /locus_tag="ACG8KR_000609"
                     /pseudo
     CDS             complement(714..1276)
                     /locus_tag="ACG8KR_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349929.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - obsolete protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
     gene            complement(2008..2589)
                     /locus_tag="ACG8KR_000610"
     CDS             complement(2008..2589)
                     /locus_tag="ACG8KR_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFQ0200010.1"
                     /translation="MKTKEKILEATKDLIYKKGYHETSIRDILAASDTGKGQFYYYFS
                     SKKEVCLAVIHAHMDVWQTELFEDILNAGKQPEEALEEMLDWIFADHETQITYHGCPV
                     GNLIIELSTVDEEYRLLLLELVNQWVKYTKQNILALSEEKLSDEEADRQAKALIAEIQ
                     GSILLLKVTQDLSLLRQNFQLIKERLLTSQKVL"
     gene            complement(2772..3398)
                     /locus_tag="ACG8KR_000611"
     CDS             complement(2772..3398)
                     /locus_tag="ACG8KR_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HFQ0200011.1"
                     /translation="MFNNKNTAVVLTDPQVEFLKPAGGGFGLTKDILEKYNTIENMIE
                     MVKQAKEKGYKLFISPHYFYPHDHKWQFGGAGEHMMLEQQMFARKSQYESVEEGSGAD
                     FIEELKPYLDENTIISSPHKIFGPESNDLALQLRKNGIDTVILGGMNGNLCSDSHMRE
                     LVESGFNVVVATDAIGAPGQEAMDAALTNFGFIADQVMTTEEVLEQLS"
     gene            complement(3420..4751)
                     /locus_tag="ACG8KR_000612"
     CDS             complement(3420..4751)
                     /locus_tag="ACG8KR_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016668 - oxidoreductase activity, acting
                     on a sulfur group of donors, NAD(P) as acceptor [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HFQ0200012.1"
                     /translation="MMEKYQNIVIGFGKGGKTLAKALASKGESVLVVEKSTRMYGGTC
                     INIGCIPSKSLIFNGERGIDFTEAVARKEKLTGMLRAKNYHMISDEATGTVMDGTARF
                     LSNHQIEVTNNGEKVIVEGERIFINTGSEPIILPIKGLNTSRYLIDSTQAMDQEQLPE
                     KLVIIGAGYIGLEFASMFNEYGSKVVVLDAHSEFLPREDEDIAQMILEDMTNAGIEFH
                     LGVSVDQVADQESTAAVTFTEDGQEVTIQASKVLAATGRKPSTASLGLENTDVKLTDR
                     GAIAVDEYLRTDADNIWAIGDVKGGLQFTYISLDDYRIIMDQLKGENKRTTNNRKAVP
                     YSVFITPTLSNVGLTEKQAKAQGVNYKLFKYMTSGVPKAQVLEDPKGVFKVLVDPETD
                     LILGASIYAEDSHEVINLISLAMNGKLPYTLLRDQIYSHPTMSEALNDVLK"
     gene            complement(4906..5307)
                     /locus_tag="ACG8KR_000613"
     CDS             complement(4906..5307)
                     /locus_tag="ACG8KR_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HFQ0200013.1"
                     /translation="MKKAYETAMINHGGRNGEVEAPNGSMHMKITPPGIHAEGTNPEQ
                     LFAAGYASCFNGAVQHMIKEAGMTAESTVKARVSLYNLDDGGYQIGVVLEVHIDGISE
                     EEAQKIAEDAHEFCPYSKATRGNIDVEVTIV"
     gene            complement(5393..5599)
                     /locus_tag="ACG8KR_000614"
     CDS             complement(5393..5599)
                     /locus_tag="ACG8KR_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0200014.1"
                     /translation="MVTNMVIFLTVLVYGVNFLVYMYLKINHIKDYVEKIAIYFGTNM
                     TLLFTSSIFLFFGKIVEDGILVFE"
     gene            complement(5694..7196)
                     /locus_tag="ACG8KR_000615"
     CDS             complement(5694..7196)
                     /locus_tag="ACG8KR_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HFQ0200015.1"
                     /translation="MKDMKALQNGSDIRGIALPVEGYEVNLTTDMVKKIGWGLANWIK
                     KKKKSTDPNKKWTIGIGHDSRISGPALKEALIQSLKEQGIHVIDFELATTPAMFMSTQ
                     FEQFSCDAAVMLTASHLPYYYNGIKFFTSEGGAEKEDIAFILSQQQKIKSEQTGSVEK
                     ADLLTPYAQDLVTKIKKGIGTLESKPLNGWKIIVDAGNGAGGFFAEKVLNSLGADSTG
                     SQFLEPDGRFPNHIPNPDNKEAMESIKQAVLANEADLGIIFDTDVDRSAVVSASGTVI
                     NRNNLIAVLSTIVLKEHPGTRIVTNSPTSSHLQRFIEEKGGIQVRYISGYRNVINKAL
                     ELNRLGKDTQLAIETSGHAAFKENYFLDDGAYVIAKILMLLPELKRKGITLDEQIKEL
                     KQPVEVQEIRLKLFSDEPREQGNKILEEMKRLLEEEKGMNFDPENEEGIRYDLEEPYG
                     NGWFLIRLSLHEPLLVLQIENDEFGKNKLALVKLAKLFGHFSDLESLTAE"
     gene            complement(7214..7669)
                     /gene="argR"
                     /locus_tag="ACG8KR_000616"
     CDS             complement(7214..7669)
                     /gene="argR"
                     /locus_tag="ACG8KR_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HFQ0200016.1"
                     /translation="MKKKQRQALIRQIITEQPIGTQEELLARLHEAGADVTQATISRD
                     IREMKLVKSQNENKIVRYTLFNQPSVSLNEERLRTAIRREVLRIQSVQFMVIVLTERN
                     GADVVTNWLDEVAYPEVVGTMAGVDTFIIICRSEEEAQRFAEKLEKMRE"
     gene            complement(7767..8384)
                     /locus_tag="ACG8KR_000617"
     CDS             complement(7767..8384)
                     /locus_tag="ACG8KR_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFQ0200017.1"
                     /translation="MEKIEWRKDKSYSMKKSPQILEVPKKSFFSIRGIGDPNQEDFKR
                     RVACLYAVSYAIRMSPKKNWLIPDYQMYTVYPLEGQWGLQEKYLHEPVMKKEHFSYQL
                     MIRQPDFVTEAIAQEAIQRSSKLPEDLKEQVVFKKMEEGLCAQILHVGPYAEEPATFE
                     KLEAFIAKEGYERASKEHKEIYISDPRKSAPEKMKTILRVKIEKG"
     gene            8605..9978
                     /locus_tag="ACG8KR_000618"
     CDS             8605..9978
                     /locus_tag="ACG8KR_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFQ0200018.1"
                     /translation="MTKQKQLLLVPAVIATGMMSFAGVLIETAMNVTFPTLIRQFDLT
                     TAQVQWVTTIYLLMISIIVPLSTYLNRNFSLKKLFLASNLLFLAGIMIDFFSPTFMVL
                     LFGRLLQGAATGIALPLMFHIILTFAPLNRRGAMMGVGTLTTAIAPAIGPTYGGIMTS
                     HFTWNHIFLFLVPVLFLSLIIGWFAIPEMAVQKSGYLDWISVLGICLLFSGSLTFLST
                     LGTVSSWIALIIGGIGWFLFYYRSKKVEHPLIRLSILSNKTFRLFLIGFLVYQFLLLG
                     VSFVLPNFLQIVQGSSPFVAGLAMLPGAAVGALLSPFSGKMLDQYGPKKPIFAGLILS
                     LIGWLALFLLIGHASVGVLIACHVFYMIGIGLSYSNMMTTGMNALNEELQGDGNAMFN
                     TLQQFSGAVATSLVAVIINYVQHHTSHNYEVSTTLGSKAALGVLLLLLLVSFARFAYY
                     LFFAKKA"
     gene            10053..11165
                     /locus_tag="ACG8KR_000619"
     CDS             10053..11165
                     /locus_tag="ACG8KR_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFQ0200019.1"
                     /translation="MEKIAIIGGGIIGMTLASYLDTSKFNVALFDEGTGQATKASAGI
                     ISPWLSKRRNKQWYRLAKDGAAFFPKLIKDFQLDQTIYAQSGTLLLRPEKDLAELAVL
                     AEKRKEEAPEIGNIEWLSPEQTSQALPLLKPAVSLRISGGGRLDGKAYLEHLGQRLAQ
                     KQMEQIQAKVTLHKKSDSWLIKGPFGEYTADRVILTPGPALKPLLQELGYAADIRPQK
                     GQLLAFQTSYKMSQDWPVAMLDGEADFIPFMDGKILLGATHENDQGWDLAPTSEAYKG
                     LTKSTEQFLTDPDQIKNWPYEFRVGTRAYTSDFSPFFGVLPDDPSLLAASGLGSSGLT
                     TGPYIGYLLANFLNTGHWPTEDYQKDISTYITKNPS"
     gene            complement(11237..12874)
                     /locus_tag="ACG8KR_000620"
     CDS             complement(11237..12874)
                     /locus_tag="ACG8KR_000620"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HFQ0200020.1"
                     /translation="MSTKESLELNEITSGNPLLSSIRSIVETTFYGNNVHEVFDRKTA
                     YQLAKGSPGTIITDLTISHAEELDLPADVRTLVFNDGSIVGRTASARRIFEDLDKEQS
                     KYEKILREAVYQSRKRQFYHTKVIVGLSEEFSVQSHLLIPEGFENNLYSYLLNFQPAN
                     SKILETYQKSVSFDEGDLYIFCDPEWTHETFPNGLILFDALHNTAAVLGLRYFGELKK
                     ATLTLAWALGNRNGFIACHGGMKQYASNDQTYTMAVFGLSGSGKSTITLAKHTEGHSV
                     SILHDDAFVISRKNGFATALEPSYFDKTQDYPSDDPAISSFLTCQNVGVTCTSKGEKV
                     LVTGDIRNGNGRTVKSRYATPDRVDHLSEKIDAVFWIMKDDSLPPIIKINDPVLAASF
                     GATLATKRSDAENITTKRQLDELVIEPFANPFRVYPLREDYQAFRELFLKQQTACYVL
                     NTGYFDDKKIKPETTLSIVERIVQQTAEFKSFGPFEDIHYLPIPDFPVSFEDTSYKNL
                     VKQRLDIRLRFIEAQIGKDLLPDEAKESLENLIQKLN"
     gene            complement(13042..13650)
                     /locus_tag="ACG8KR_000621"
     CDS             complement(13042..13650)
                     /locus_tag="ACG8KR_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFQ0200021.1"
                     /translation="MNSFKKIVLGTTFAAGATAMFVGTTNAHADEVYTVKSGDSLSKI
                     SQKFAGDNSMIDAIAEKNSIANINRIYVGEQLTIPTSNDSSATTENKTTENTASTTET
                     ATQEHTYVAPVETVEVAPAAPAAATAPTSSSAKEWIAQKESGGSYTATNGRYIGRYQL
                     DASYLNGDYSAANQERVAEQYVTSRYGSWDAAKTFWLANGWY"
     gene            complement(14034..15185)
                     /gene="queG"
                     /locus_tag="ACG8KR_000622"
     CDS             complement(14034..15185)
                     /gene="queG"
                     /locus_tag="ACG8KR_000622"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HFQ0200022.1"
                     /translation="MTLKEEIIQESKRLGIDKIGFTTAEPFDSLKESLEEQKAAGHTS
                     GFEHPNIDERLYPEMTFDQPKSIISIALAYPTRIHEEVPRDEKRGQFARASWGIDYHD
                     ILRDRLNRLIEFIQTRAEKWQKEEEWRLAPQVDTGELIDVAVAQRAGLGFIGRNGLLI
                     TEEFGSFVYLGEIVTNIVFEPDKPGEFGCGDCMRCVTACPTKALLGDGRMNAQRCLSY
                     QTQTKGMMPEEYRKKMRNVIYGCDICQLVCPYNQGKDFHFHEEMEPKVDEIYPKLKPM
                     LSMSNKDFKQQFGHLAGSWRGKKPLQRNALIALANLGDRTALPEISECLTDVRPVIRG
                     TAAWAIGRLGTKEPEKWLDVLDEILAKETEEVVINELEHAQKLLRRKLK"
     gene            complement(15204..15956)
                     /locus_tag="ACG8KR_000623"
     CDS             complement(15204..15956)
                     /locus_tag="ACG8KR_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0200023.1"
                     /translation="MNFTRKDKYTIKEEVFRLISQQENTDKDQDLQFLEAELAKLFIA
                     NQNETTEPDEELYIGKWLFDEFQPLDENTGIGYFVFLSPVGFTPGVLVKQNDEITVKA
                     WPALFSVIKAEGVIKKIQSAYYKSQFIANSKLMNMFYNRLVEIARPGKIPQTFTYTQQ
                     KNLYEEVLYSRDLSEFSPWEEVRLHELLSTKPVSLNFFNALDNKRKLISTNYWEVFAA
                     RKKWMEEKKIADQQIQFVQLKKEKLYYGLYSR"
     gene            complement(16097..17047)
                     /locus_tag="ACG8KR_000624"
     CDS             complement(16097..17047)
                     /locus_tag="ACG8KR_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HFQ0200024.1"
                     /translation="MKTKKSLFLILAVSFLVLAGCGKQASDQADEGSKEKLSVVATNS
                     ILADMAKEVGTDQIDIHSIVPVGTDPHEYEVLPEDIKKASDADVILYNGLNLETGNSW
                     FDNLMETAKKEEGKDYFAVSKNVEPLYLTSGEEHTKADPHAWLDLSNGIKYVEEIARI
                     FSEKDAENATLYKKNAEAYVEKLKELDTQAKESFASIEENKKLLVTSEGAFKYFSRAY
                     DLPAAYIWEINTESQGTPDQMKAIIDQIRAKEVPVLFVETSVDSRSMERVAKETGLKI
                     YDKLFTDSIAKEGEQGDSYYQMMKWNIETIHEGLSQTKES"
     gene            complement(17048..17887)
                     /locus_tag="ACG8KR_000625"
     CDS             complement(17048..17887)
                     /locus_tag="ACG8KR_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFQ0200025.1"
                     /translation="MIQSFIDGLMNYQFLQYALVTSMLVGLASGVIGSFIILRGMSLM
                     GDAISHAVLPGVAISYMFGFSYIFGATAFGMLTAAVIGFVTQRSRLKNDTAIGIVFSS
                     FFALGIILISYAQSATDLYHILFGNVLAVRESDLLLTALVSGIVLIFVFFFYKELKIT
                     SFDPTMAKAYGLNTSLIHYLLMFFLTLVAVVSLQTVGTILVIAMLITPAATAYLLTNH
                     LLKMIITAAGIGMLSAVVGVFFSYSYNWPSGATIVLACTAFFILAFLFSPTKGILFQK
                     GSR"
     gene            complement(17884..18630)
                     /locus_tag="ACG8KR_000626"
     CDS             complement(17884..18630)
                     /locus_tag="ACG8KR_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HFQ0200026.1"
                     /translation="MERTAIVIQDLSAAYQGKTVLKTINLTINPQKITGIIGPNGAGK
                     STFMKSLLELIPATTGKVTFSGQSVNSVRKKIAYVEQRSELDLSFPIDVLGVVLLGTY
                     PSLRIGQRPGKKEKERARQALKKVGMEEYAKRQISELSGGQLQRVFIARALAQGAEWI
                     FLDEPFVGIDALSERKIFDILQELKNSGKTILIVHHDLHKVEEYFDEVILLNKQLIAS
                     GPVQEVFTSKNLQLAYGEIIRHLVKGGEKK"
     gene            18868..20238
                     /locus_tag="ACG8KR_000627"
     CDS             18868..20238
                     /locus_tag="ACG8KR_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFQ0200027.1"
                     /translation="MEEEKLTRSLSARHIQMIALGGTIGVGLFMGASSTIRWTGPSVM
                     GAYAIAGFFLYLIMRALGEMLYVDPSTGSFANYASEYIHPVAGYLTAWSNIFQYIVVG
                     ISEVIAVGSYMNYWWPDLPAIIPGIIVVLFLMLANLISVKAFGELEFWFSIIKVITII
                     LMIIAGLGVILFGFGNHGQAVGISNLWKNGGFFTGGVKGFFFALSIVVASYQGIELIG
                     MTAGEAENPKKTIIEAVQSTIGRILIFYIGAIFIIVSIYPWNQLSEIGSPFVQTFSKV
                     GITFAAGLINFVVITAALSGCNSGIFSASRMVYTLAINGKMSKKFLKLSRHGAPFYPV
                     VAISGGILIGLILNYLLPMFINQSENIFVFVYSSSILPGMVPWVVILISEIRFKKKHA
                     DKMHDHPFKMPLSPYTNYVTLAFLAIVLIFMFINPETRISLIIGLVFLLYMTIHYFVR
                     EKRTGK"
     gene            complement(20293..21189)
                     /locus_tag="ACG8KR_000628"
     CDS             complement(20293..21189)
                     /locus_tag="ACG8KR_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFQ0200028.1"
                     /translation="MYDENEWGENVRMTRRRQQKQAARKRMIMIGAAVAVFLIIAGGV
                     AGLYFHGKNSSEAKSTTITETTTKKTETKQTSESSTKTKETQTSEEKTANGLPEQLQA
                     IVNDYSKKIEEKTPALIEEYQTEIQGNQEGIAGLSAVANQKARELQAISDEGIRKLRA
                     AYQAAENKDGVDLDTLINQLSANYTNHVAKISDIYLQTSASLQAESTSTQDTTSSDSE
                     TAESVEASEMQARTTQDTTDSSETNTNSATSQAATTVVLEGEGPNQIAERTGVPVETI
                     LSLNGMSMENYFLTPGETLKLN"
     gene            complement(21303..21473)
                     /locus_tag="ACG8KR_000629"
     CDS             complement(21303..21473)
                     /locus_tag="ACG8KR_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0200029.1"
                     /translation="MTNDWLIQQLDELSEKSEYQDRALLYEIKKLMAEIDERTMQLQG
                     EIDGQSWNHLNW"
     gene            complement(21470..22147)
                     /locus_tag="ACG8KR_000630"
     CDS             complement(21470..22147)
                     /locus_tag="ACG8KR_000630"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008753 - NADPH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HFQ0200030.1"
                     /translation="MRTLIIISHPTIEESLNQQFFKEASAGLPATWHHLESSYPDGKI
                     DIETELQLLAQHDRIIFQFPFYWYSSPAHLKIWQDNVLERAAHVLKGKELGIVLTTGV
                     AEKEYQAGGKEEYTISEFLRPYQRIANKFHMTYLPPFVLAQFMYLSQEKRWEKLIAYQ
                     QYLSLEGKPSLTQRIDWFIQRVQENQKMQEEESEKQAYIVEALTEAKEQIEDLTFTLQ
                     EMKGTSL"
     gene            complement(22238..23143)
                     /locus_tag="ACG8KR_000631"
     CDS             complement(22238..23143)
                     /locus_tag="ACG8KR_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family ligand binding domain-containing
                     protein"
                     /protein_id="HFQ0200031.1"
                     /translation="MEQSIFLKKEHQQVEKELYFDFCGFSKTMPFHSFGPAIRNDYIL
                     HVVMEGKGSYHVKNQQFQLKKGDLFLIRPGDSTFYLSDGEDPWMYCWLSFGGSAAEEI
                     IRQSLFREDQYTMVSSDIQKYIDIILECMKDTEETLENELQLTALTYRFLSVLLKDGG
                     KVSGGVQKVHSSLAVETVKYIEAHYAEKLSVEEIAQHLAVNRSHLSRVFKNHMGTSIK
                     EYLIGVRINRGAFLLSLTDESVESIAYQVGFNSLVVFSRMFKKSTGETATNYRKRMKN
                     ENFKNIALDTLKKQLEKQTAVSWTT"
     gene            23318..24742
                     /locus_tag="ACG8KR_000632"
     CDS             23318..24742
                     /locus_tag="ACG8KR_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015486 -
                     glycoside-pentoside-hexuronide:cation symporter activity
                     [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide (GPH):cation
                     symporter"
                     /protein_id="HFQ0200032.1"
                     /translation="MKSKMIQRVSYALGAFGHDTFYVTLSTYFMVFVTSQMFAGTDKA
                     TNAKMIATVTSLVVGIRLVEIIFDPLIGGIIDNTRTKYGKFKPWLVIGGVVSSVLLVA
                     IFTNFFGLATHNTTLFIILFAIVFILLDSFYSFKDIAFWSMIPALSTETKEREKFATF
                     ARFGSSLGANGTTLVVVPIVTFFTYLVTHKHIEGASGWFWFGVIAAVISGGTAIITAL
                     GTKEVDTAIRQQNEKAGIKDVFKAVAQNDQLMWLALAYIAYAIANAATTAVLFYFFKY
                     VLGQPTEFWIVGLVAALLGLVAVPLFPMLTRVISRRYVYLGGIAMMISSYFFFTIAGS
                     SLPIVIIGLVLFYFPQQLIFLSALMTITDSVEYGQWKNGIRNEAVTLSIRPLLDKIAG
                     ALSNGIVGFVAIASGMTGNATAADITAHGVTTFKAYAFYAPAALMIFSAIIFAWKITL
                     TEKKHAQIVEKLENKLAPADTMED"
     gene            24788..25819
                     /locus_tag="ACG8KR_000633"
     CDS             24788..25819
                     /locus_tag="ACG8KR_000633"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HFQ0200033.1"
                     /translation="MKITEKDFGQGYHLITLENKNKLALSISDLGARIVSLKSNDREL
                     VLGFDTAEEYIEKDPYIGASIGRTAGRIENGRFSLNGKTYQLATDPKTGHNLHGGAPG
                     FELKKWSYVILNGENEASVIFTTTSPDGEHGFPGTMDVEIRYTLTKDNIWRVTSRGTS
                     DQDTLFNPTNHVYFNLTGDASQSIDQHELWLNSEAYAPLRTDSIPIGVKENAAGSAFD
                     FQIPKKLASVFASDLDQKNLVDGIDHPFFLKETGLGKEAARLTSPDKRIQVDIATDAS
                     SVVIFTANFGTETPEMRNRKLAHHGGITFETQTAPGAERFSAFGSIHLKAGSVFETVT
                     EFKIKTRKE"
     gene            25826..27013
                     /locus_tag="ACG8KR_000634"
     CDS             25826..27013
                     /locus_tag="ACG8KR_000634"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046835 - carbohydrate phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HFQ0200034.1"
                     /translation="MEIKRSLNTKFAETFGPKKTTQYFSPGRINLIGEHTDYNGGHVF
                     PASITYGTQGVAAPRDDNKILVYSTNFDDIGVISFTLDELEYDKKANWANYVKGMVLK
                     LKEAGHSIDHGFELLVEGTIPNGAGLSSSASLELLVGVVLEDLFDLKTDRLELVQTGK
                     KVENEFIGVNSGIMDQFAIGFGELDHAILLDTNTLKYEMVPVKLDGYAIVIMNTNKRR
                     ELADSKYNERRSECEEALARLQKTLPISSLGDLDEEEFESTIDQIGDDTLIRRARHAV
                     SENQRTLKAKAELEAGNLEAFGQLLNDSHHSLRYDYEVTGIELDTLVDAAQKQEGVLG
                     ARMTGAGFGGCAIALVKEENIPAFKNNVYDEYMKVVGYAPEFYVAHIGNGTTKIDPEA
                     VHV"
     gene            27169..28551
                     /locus_tag="ACG8KR_000635"
     CDS             27169..28551
                     /locus_tag="ACG8KR_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HFQ0200035.1"
                     /translation="MWANQNPVFVYEKEPSRDILCIDCKSFYASVECVEKDLDPLTTK
                     LVVMSYPSDSTETRGSGLILASSPAAKKAYGITNISRARDLPFPYPSDLYIVAPRMAY
                     YMEKNREINSIYKKYADEANHHVYSVDESFLDVTNSMALFGKTNAYDMAKLIHKDVFE
                     QTGIYTTIGIGDNPLLAKLALDNGSKDGKQMFAEWRYEDVPEKLWKISDITDFWGIGK
                     RTAKRLKQMGIHSVYDLAHADYYHLKSKLGVIGTQLYAHAWGIDRSFLGQEYTPKSKS
                     IGNSQILDRDYTRREEIEIVIKEMADQVGTRLRRSAAKTQVVSLWVGYSLGYLDPNGK
                     TGFHQQMKIEATNASRDLADALLTIFHQHYHYQDIRRVGVNCSDLVYATGLQLNLFED
                     PEKQINEAKVDFVVDTIRKKYGFQSIIHAHSLLQGGRAIARSSLVGGHAGGMSGLEGG
                     KDETDSKKIH"
     gene            28588..28908
                     /locus_tag="ACG8KR_000636"
     CDS             28588..28908
                     /locus_tag="ACG8KR_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0200036.1"
                     /translation="MGDCFCDGRTSQRYQKNEEEARKQPLPKQQMTLQEIDSILTEAF
                     LSRKAVIIQLNLKDEFGRLLDEVEGHFIGEAYEDYFMIDHQPILWEDVRYIELKETKK
                     WFVT"
     gene            complement(29108..29776)
                     /locus_tag="ACG8KR_000637"
     CDS             complement(29108..29776)
                     /locus_tag="ACG8KR_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HFQ0200037.1"
                     /translation="MIFSITMKLVFGLIGLLLVVRLLGKKSMSEITPFDLVYTLVLGG
                     ILEESTYDDNVHVGHVLFAIALWAVMIYGIERIVQKNEKVNRWVKGEPSVLIKDGVIN
                     MTELTNNHIEMEQLRAILRQQECFFLENAKHVILENAGQMSVLKKSDEDKALSILLVD
                     EGQIQHKVLQSNQLTEAWLMENLKKEGYADVKQLIYVEWSEEKGLYILNKEDVMHESY
                     RIDG"
BASE COUNT         8412 a         6144 c         5476 g         9969 t
ORIGIN      
        1 aaataaattg tagccagttc attttttctt taactaaaaa aataatccaa gaaacattga
       61 acctatggat ccaaaagcta cccaaatact atagctaacc ccccgctacc ttcaattttg
      121 cagataaaac gctttgggag aaaatttcaa agacaacagg ccatcctatc atgcccgatt
      181 ataaacaaaa agctggctat tccttttgct ctgacaaatt tcaattggga aagttcacga
      241 aactagcagg tgaagaagtt tcaactgttc ggatcaaaga atgcccgctt caaatagaaa
      301 cattggttca acgaatttca actagacaag actttgctat tgtagaacgt gagatcgtag
      361 ctatttttgt agaagaaggg attttacatg atccaactca tatcaatgtg gataaatggc
      421 aacctcttat ctataaattc agagaatata caactgccaa ccagtcgtta actttgaatt
      481 ttagattcca agaattcaaa gagtcataat gataaacatc gttaggtata aataaaaaag
      541 actcgcagca cgagtaaaaa acaaatagaa gctatacatt agaaaggtcg actgtgaccc
      601 tatagatgca gtatatgcag cttatgagca tttcttctgt tctctttgtt tttgggatct
      661 gtgctgaagt ctttttcgtt ttttgccgct ctttctttga aaaatcgatt atgttatacc
      721 ttctccatta ctaattcagg catctgctga gtaatgattt ttcgcgcaaa agaaatatct
      781 gttacccaag gttcgatttg ttcttcagtc aagacaagcg gcattcggtc atggattggt
      841 gcgactgtgc ggttaggttc agtagtcatt atgattgatt ctgccatttt ttcatcctga
      901 tggctacgat aaaaaccgcc aatatacact attttttcct tatttgaaaa cataaatttg
      961 tctttttctt cattccactc attgggaaaa cacaacgatt ttgcaaaaat ggttgatgaa
     1021 aggtcttttt ttcttctact gtctctgcac gggcattaat catcaattgt ccttttttaa
     1081 atccggtaaa tccccacttc atgatttccg gagtgaggat gttttcttcc tcttttctta
     1141 acgtcacaac aagatttgac ggaaaaactt ctccagctgc taattctgct gcctgcttcg
     1201 gttgtttcgt ctgggcttct cgataataat tctgaaggag ctctgactct agatcaaaaa
     1261 aataacgacc acacatccta atccctccca atcttttttt ctatcataca ctgcaacaag
     1321 aagttcgcac caacaaaacg ctttcaattt ttgtagaact aaaaaagttg tgacaagtga
     1381 caaactctcc agtgaatggt attctcctgc caactcctcg gtcacaagtc acaatattca
     1441 acaaacatga gaagctgctt tttgaaattg ttccttgtga ctcggagtga aacggcaggc
     1501 ttacgacctc tgggtaaacg gtgttcaaaa gccagtttct cggtattaag ccaaatttat
     1561 gcaaaaatgt agaaagctat tttcacaaaa tttttcttaa tactcgaaac taagcgactt
     1621 tttcacaacc tcttccttaa acggtgttca aaagcttgca tctgcggtaa taagcccaac
     1681 caaacgaaaa atatgagcag ctatttttat ttggctgttc ttattacttg atgcagggca
     1741 gctttttcac aacctctcca ctaaacggtg ttcaatcagc taaccttcgg tattagctca
     1801 aaaaacggca aaatatgagg aactattttg ccgtttgttt ctctaatact caggtcaaag
     1861 cgctgatttc acaacctcta atacacggtg ttccagaagt agcttcttcg aaaaataagc
     1921 cgaaatttca caaaaatttg agaagcaatt ttcgtaaatt ccttcttatt tcttgaagct
     1981 gaccacttct gtcacaacct ctaattatta aagaaccttt tgcgaagtta acaatctttc
     2041 tttgatcaat tgaaaattct gcctaagtag tgatagatcc tgtgtgactt tcaacaataa
     2101 gatacttccc tggatttcag caatcaacgc tttggcttga cggtctgctt cttcatcgga
     2161 cagtttttct tcagaaagtg ccaggatatt ttgtttcgta tacttgaccc attgattgac
     2221 taattctagt agcaacagac ggtattcttc atccactgta ctcaactcaa taatcaaatt
     2281 acctacagga cagccatgat aagtgatctg tgtctcatga tcagcgaaaa tccagtccaa
     2341 catttcttct aatgcttctt ctggctgttt tccagcattt aagatgtctt cgaacaattc
     2401 tgtctgccat acgtccatat gcgcatggat caccgctaga caaacttctt tttttgaaga
     2461 aaagtaataa taaaattgac cttttcctgt atcagaagct gctagaatat ctcggattga
     2521 cgtttcatga tagccttttt tataaataag atctttggtc gcttccaaga tcttttcctt
     2581 tgttttcaat gatattctcc tttttcattt ctcatagtcc tatattatac agtaaattca
     2641 tcattttttc ttcttaatcg tttaaaaaag actgtgccaa attatctgac acagccctca
     2701 ctataaaact gttctcatat agctagatgt tcattgattc aagaactgcg aggacaaaag
     2761 caattcctat cttatgatag ttgctctaat acttcctctg ttgtcatcac ttgatctgca
     2821 ataaagccaa agttcgtcaa cgctgcatcc attgcttctt ggccaggagc accaattgca
     2881 tctgtcgcta ccacaacatt gaatccactc tctaccaatt cacgcatatg ggaatcgcta
     2941 cacaagtttc cgttcattcc accaagaatt actgtgtcga ttccattttt tctcaattgg
     3001 agcgccaaat cattgctttc tggtccaaag attttatgag gagagctgat gatcgtattt
     3061 tcatccaaat aaggtttcaa ttcttcgata aaatcggctc ctgaaccttc ttccactgat
     3121 tcatattgac tttttctagc aaacatctgc tgctcaagca tcatatgttc tcctgcaccg
     3181 ccaaattgcc atttatggtc atgtgggtaa aaataatgag gagaaataaa gagtttgtat
     3241 cctttttctt ttgcttgttt taccatttca atcatatttt cgatcgtatt gtatttttct
     3301 aagatatctt ttgtcaaacc aaagccaccg cctgctggtt tcaagaactc gacttgcggg
     3361 tcagtgagaa ctactgctgt atttttatta ttgaacatgg tggattctcc attcgttatt
     3421 tattttaata catcatttag agcttcactc attgtcggat gagaatagat ttgatctctc
     3481 aataacgtat aaggaagttt tccattcata gctagcgaaa tcaagttgat gacttcatga
     3541 gaatcttccg catagatact tgcccctaga atcaagtcag tttcagggtc gactaaaact
     3601 ttgaatacac ctttaggatc ttctaatact tgtgctttag ggacaccgct tgtcatatac
     3661 ttgaataatt tgtaattgac gccttgtgct ttggcttgtt tttctgttaa acctacattg
     3721 gataaagtcg gagtgataaa tacactgtaa ggcacagctt tccggttatt cgttgttcgt
     3781 ttattctcgc ccttcaactg atccataatg attcgataat catctagaga gatataagtg
     3841 aactgcaatc cgcctttgac gtctccaatc gcccaaatgt tgtctgcatc tgtacgaagg
     3901 tattcatcca cagcgattgc tccgcgatct gttagtttaa catctgtatt ttctaatcca
     3961 agtgatgctg tacttggttt tcttccagtt gcagccaaaa cttttgacgc ttggattgtt
     4021 acttcttggc catcttctgt aaaggtgacg gcagctgttg attcttggtc tgctacttga
     4081 tctacagata cacctagatg gaattcgatt cccgcatttg tcatgtcttc taaaatcatt
     4141 tgcgcgatat cttcatcttc acgtggaaga aattcagaat gggcatcaag aacgacaact
     4201 ttagatccgt attcattgaa cattgatgca aattccaagc caatatatcc agccccgatg
     4261 atcactaatt tttccggtaa ttgttcctga tccatcgctt gcgtagaatc aatcaagtag
     4321 cgacttgtat tcaatccttt gattggtaaa atgattggtt cagaaccagt attgatgaaa
     4381 atccgttcgc cttcaacaat cactttttca ccattattcg tcacttcgat ttgatggtta
     4441 gaaagaaaac gtgctgtacc gtccatcact gtcccagttg cctcatcgct gatcatatga
     4501 taatttttgg cgcgaagcat gcccgtcaat ttttctttcc gagcaacagc ctctgtaaag
     4561 tcgattcctc tttctccatt gaaaatcaat gatttagaag gaatacagcc gatgttgata
     4621 catgttccgc catacatacg tgttgacttt tcaacgacta aaaccgattc accttttgaa
     4681 gctaacgcct tcgctaaagt cttaccgccc tttccaaaac cgatcacaat attttgatat
     4741 ttttccatca tttttccctc gcttcttttt tacctaccga ttggtacaaa aatatgataa
     4801 ccgataattg tctattctcc aaatcatctg ctttatttgt caaaaaaaac cagacaaaaa
     4861 gcggtccttt cacgttttta gtctggttcg atttccttta ttcttctaaa caatcgttac
     4921 ttctacatcg atatttcctc tagttgcttt ggagtaaggg cagaattcat gcgcatcttc
     4981 tgcaattttt tgggcttctt cttcgctgat gccatcgata tgcacttcta atactacacc
     5041 gatctgatag ccgccatcat ctaaattata aagagagaca cgtgctttta ctgtggactc
     5101 tgctgtcatt cctgcttctt tgatcatatg ttgtaccgcc ccattgaaac aagaagcata
     5161 acctgctgca aataattgtt ctggatttgt tccttctgca tggattcctg gaggagtgat
     5221 tttcatatgc atcgagccat ttggtgcctc gacttctcca ttgcgcccgc catggttgat
     5281 cattgctgtt tcgtatgctt ttttcattgt actgctcctt tcgattgacc atttgctact
     5341 tggctataat tatagtgtat gacaagtaag gcgctttcta caaaaattct gcttattcga
     5401 ataccagaat gccgtcttct actatttttc caaaaaacaa aaaaatacta ctcgtgaaca
     5461 acagcgtcat atttgttcca aaataaatag caatcttttc tacataatct tttatatgat
     5521 tgattttaag atacatatat accagaaagt tgaccccata aaccaatact gttaaaaaaa
     5581 taaccatatt cgttaccatt ttttcctcct tttgctggtc tccctaccag cactcgattt
     5641 aaccatacca ctttttttgc cgtccaagca actagccttt caaaaagact ggtttattct
     5701 gctgttaatg attcaagatc agaaaaatgt ccgaataatt ttgctaattt tactaacgcc
     5761 aacttgttct ttccgaattc atcattttct atctgtaata ctagtaacgg ctcatgtaag
     5821 cttaaacgga tcaaaaacca gccgtttccg tatggctctt ctagatcata tcgaatacct
     5881 tcttcatttt ccgggtcgaa gttcatccct ttttcttctt ccaacagtcg cttcatttct
     5941 tctaatatct tgtttccttg ttcgcgtggt tcatcgctga acaattttaa tcggatttct
     6001 tgtacttcta cgggctgttt caactctttg atttgttcat ctaatgtgat accttttctt
     6061 tttaattctg gcaaaagcat caggatcttt gcgatgacat atgcaccatc atccagaaaa
     6121 tagttttcct tgaatgcagc gtgtccgctt gtttcgatgg ctagctgtgt atctttccca
     6181 agacggttaa gttctaatgc tttattgatt acatttcgat agccggaaat ataacgaact
     6241 tggatgcctc ctttttcttc aatgaatctt tgtagatgag aagaagtagg ggaattcgtc
     6301 acgattcttg ttcctggatg ctcttttaat acgattgtac ttaatactgc gatcaaatta
     6361 tttcgattga tcactgttcc agaagcactg acaactgcag aacgatccac atctgtatcg
     6421 aaaatgatcc caagatctgc ttcatttgct aaaacagcct gcttgatact ttccatcgct
     6481 tctttattat caggatttgg gatatgatta ggaaatcggc catctggttc caaaaattgg
     6541 gaacctgttg aatccgcacc taagctattc aatacttttt cagcaaagaa tccaccagca
     6601 ccatttccgg catcaacaat gattttccaa ccatttagtg gtttgctttc tagtgttcca
     6661 atcccctttt taatttttgt gactaaatct tgcgcataag gtgtcaaaag gtctgctttt
     6721 tctacgcttc cagtttgttc gctttttatt ttttgctgct gtgataggat aaaagcaata
     6781 tcttcttttt ctgctcctcc ttctgaggta aagaatttta ttccattata ataataggga
     6841 agatggctag cagtcagcat aaccgcagca tcgcacgaga actgttcaaa ttgagtggac
     6901 ataaacatag caggcgtagt agctaattcg aaatcgatga catgtatccc ttgttctttc
     6961 agactttgga tcaacgcctc tttcagtgct ggtccgctta tcctactgtc atgaccaatc
     7021 ccaatcgtcc attttttatt tggatcggtt gatttcttct ttttctttat ccagtttgct
     7081 agtccccagc cgatcttttt taccatgtct gtcgtcaagt ttacttcata tccttcaact
     7141 ggtaatgcga ttcctcgaat atccgatcca ttttgcagcg ctttcatatc tttcatataa
     7201 aagtcctcct catttattcc ctcatttttt ccagcttttc tgcaaatcgc tgtgcttctt
     7261 cttctgagcg gcaaataata atgaatgtat ctacaccggc catcgttccg acaacttcag
     7321 ggtaagccac ttcatctagc caattcgtta ccacatcggc accatttcgt tcagtcaata
     7381 cgataaccat gaactgaaca gactggatac gcaaaacctc gcgtctaatt gctgtacgca
     7441 aacgttcttc atttaaagaa acagatggct ggttaaataa tgtatagcgt actattttgt
     7501 tttcattttg actttttact aacttcattt ctcgaatgtc tcgagaaatc gttgcttgag
     7561 tcacatcggc gcctgcttca tgcagccgcg caagcagctc ttcctgtgtc ccaataggtt
     7621 gttctgtaat gatctggcgg atcagtgctt gtcgctgttt ctttttcata ttagacacct
     7681 ctcaatgttg attatagcaa aagataagta atatttcttc atattttggt attttttact
     7741 tttatttttt gttttcattc aggttgctaa cctttttcta ttttaacacg caaaatcgtc
     7801 ttcatttttt caggtgctga ttttcttgga tcgctgatgt aaatctcttt gtgttcttta
     7861 gaagctcttt catagccttc ttttgcgatg aatgcttcta atttctcaaa cgttgctggt
     7921 tcttcggcat agggcccgac gtgtaagatt tgggcacaaa gaccttcttc catctttttg
     7981 aatactactt gttctttcaa gtcttccgga agtttgctag aacgctgaat ggcttcttgt
     8041 gctatcgctt ctgtaacaaa gtcaggctgt cggatcatca attgataaga aaaatgttct
     8101 tttttcatca ctggttcatg caaatacttt tcttgcagtc cccattgtcc ttccagtgga
     8161 tagacagtat acatctgata atcaggaatc agccaatttt ttttaggcga catgcgtatt
     8221 gcatagctga ctgcatatag acaggctact cttcgtttaa agtcttcctg attcgggtcg
     8281 ccgatccctc tgatgctaaa gaatgatttt ttaggaacct ctagtatttg cgggcttttt
     8341 ttcatactat agctcttgtc tttacgccat tcaatttttt ccatttttct tcctccgcga
     8401 aattccattt agttatttat tatcatacct ctttttgagt gacttggcta atcgtgttga
     8461 cactattttt cagtttgtgc tagaataagt gaagtaaata aatcgtacag ataacacgtt
     8521 tcggcgtgtc gagagagagg cggtttctat ttttataata gaaatcactc tcttttttta
     8581 cgaaattttt tagaggtgat gtaaatgaca aaacaaaaac agctgctttt agtacctgca
     8641 gtaattgcaa cagggatgat gtcttttgcg ggcgtattga tcgagacggc catgaatgta
     8701 actttcccaa ctcttatcag acagtttgat ttaacgactg cacaagtcca atgggtaaca
     8761 acgatttatc ttttgatgat ttcgatcatt gttccgctgt ctacttattt gaatcgcaat
     8821 tttagtctca aaaaactctt tttagcttca aatctgcttt tcttggcagg tataatgatc
     8881 gatttcttct ctcctacctt catggtccta ttgtttggtc gattgctcca aggtgcagcg
     8941 acaggtatcg ctcttccgtt gatgttccat attatcttga catttgcccc cctcaatcga
     9001 cgaggcgcga tgatgggtgt cggtactttg acaacggcca ttgctccagc aattggcccc
     9061 acttatggag gcattatgac ttcacatttt acatggaatc atatcttcct gtttcttgtg
     9121 cccgttttgt ttctttcttt gattatcggc tggtttgcta ttcccgagat ggcagtacaa
     9181 aaaagtggtt atttggattg gataagcgtt ttaggaattt gtcttttgtt tagtggaagt
     9241 ctaacatttt tgagtacgtt gggaacagtt tccagctgga ttgctttaat catcggcggt
     9301 attggctggt tcctttttta ctatcgaagt aaaaaagtgg agcatccact gatccgactg
     9361 tctattcttt caaataaaac attccgcttg tttttgattg gcttcttagt ttatcagttt
     9421 ttattacttg gtgtatcttt tgtcttgccg aatttccttc agatcgttca aggaagtagt
     9481 ccttttgtcg ctggattagc gatgcttccc ggcgcagcgg ttggtgcttt gctttctcct
     9541 ttttccggga aaatgctgga tcaatatggt ccgaagaagc caatttttgc tgggctgatt
     9601 ctttctttga ttggttggct agcattattc ctactcatag gacacgcttc agtgggtgta
     9661 ttgattgctt gtcatgtgtt ttatatgatc ggtatcggat tatcttacag caatatgatg
     9721 accaccggaa tgaatgcact gaatgaagaa ctacaaggag acgggaacgc aatgttcaat
     9781 accttgcagc agttttctgg cgctgtggct acttcattag tagcggtcat tatcaattac
     9841 gtccagcatc atacttccca taactatgaa gtgtctacta cattaggatc aaaagcagca
     9901 ttaggcgtct tgttgctgtt gcttctcgta agctttgctc gattcgccta ttatctgttc
     9961 tttgctaaaa aagcgtagat tccatatacg gcaggtgttg catctgccgt ttttttgtgt
    10021 gaaaatggtt gtttgtaaaa ggaggctttt ttatggaaaa aattgccatt atcggtggcg
    10081 ggatcattgg tatgacccta gccagttact tagatacatc aaaattcaat gttgctttat
    10141 ttgatgaagg gacaggacag gcaactaaag ctagcgctgg gattatctcc ccttggttgt
    10201 cgaaacgcag aaacaaacaa tggtatcgtc tggctaaaga cggcgctgct tttttcccta
    10261 aacttataaa agatttccaa cttgatcaaa cgatttacgc acaatcaggg acacttttgc
    10321 ttcgacctga aaaagattta gctgaattgg ctgttcttgc cgagaaacga aaagaagaag
    10381 cgccagaaat tggaaacatc gaatggcttt cacctgaaca gacatctcaa gcacttccgt
    10441 tattgaaacc agcagtttca ctgcgtatca gtggtggtgg tcgtctggat ggaaaagcat
    10501 atttagaaca tcttggtcag cgtttagcac aaaaacaaat ggagcagatc caggcaaaag
    10561 tcactttgca taagaagagc gattcttggc tcatcaaggg accgtttgga gaatacacag
    10621 ctgatcgtgt aattttgaca cctggcccgg cacttaagcc tttattacaa gaattaggct
    10681 atgctgcaga tatacgtccg caaaaagggc agctcttagc atttcagaca tcttataaga
    10741 tgagtcaaga ctggcctgtt gctatgcttg acggagaagc tgatttcatt cctttcatgg
    10801 atggaaagat ccttttaggt gctacccatg aaaacgatca aggctgggat ttggctccta
    10861 ctagcgaagc ttataaggga ttgactaaga gtacagaaca gttcttgact gatccagatc
    10921 agataaaaaa ctggccatac gagttccgtg taggtacccg tgcttatacg tcagattttt
    10981 ccccattttt cggcgtactg ccagatgatc caagcttact ggctgctagc ggtctaggct
    11041 cttctggctt gaccacaggt ccttatatcg gctatctatt agccaacttc ttaaatactg
    11101 gtcattggcc tacggaagat tatcaaaagg acatctccac ctatattacg aaaaatccat
    11161 catagaaaaa cagctttttc aagtaaggat cccgagcgat attgcgcatg gattacttct
    11221 tgaaaaagct gttattttag ttcaatttct gaatcaaatt ctccaaactt tcttttgctt
    11281 catctggcaa gagatctttt cctatctgag cttcaatgaa tcgtaaacga atatcaagtc
    11341 gttgtttcac cagattttta taggacgtat cctcaaaaga aacaggaaaa tctggtatag
    11401 gtaaatagtg gatgtcttca aatggaccaa acgacttgaa ttctgccgtt tgttgcacga
    11461 ttctttcaac gattgacaat gtcgtttcag gtttgatttt tttatcatcg aaatatccag
    11521 tattcaggac atagcaggct gtttgctgtt tcaaaaacaa ctctcgaaaa gcttgatagt
    11581 cttccctaag aggatagaca cggaaaggat tagcaaatgg ttcaatgact agctcatcta
    11641 gctgtctttt tgtagtgata ttttccgcgt ctgatcgttt tgttgctaat gttgcaccaa
    11701 aagacgctgc taaaactggg tcattgatct tgataattgg cggaagcgaa tcatctttca
    11761 tgatccaaaa aacagcatcg atcttttcgc taagatgatc gactcggtcg ggtgtcgcat
    11821 aacgtgattt gactgtccgc ccatttccgt ttcgtatatc tcctgttact aggacttttt
    11881 ctcctttact tgtacatgtc acgcccacat tctgacaagt aaggaaagaa gaaatggccg
    11941 gatcatcaga cggatagtct tgtgttttat caaagtaaga aggttccaag gcagtagcaa
    12001 aaccattttt tcgagaaata acgaaagcat cgtcatgcaa gatcgaaaca gagtgacctt
    12061 ccgtatgttt tgctaaggta atcgttgatt ttccagaacc ggacaaccca aatacagcca
    12121 tcgtataggt ctggtcatta ctagcatatt gtttcatccc accgtggcag gcaataaagc
    12181 cattgcgatt tcccaatgcc caagcaagcg tcaaagtcgc tttttttaat tctccaaagt
    12241 aacgtaatcc tagcacagcc gctgtattat ggagagcatc aaacaaaatg agtccatttg
    12301 gaaatgtttc atgtgtccat tcgggatcac aaaagatata aagatctccc tcatcaaagg
    12361 aaacagactt ttgataagtc tctaagatct tcgaattcgc tggctgaaaa ttcagcaaat
    12421 aagaatacaa attgttctca aatccttctg gaatcagcaa atgactttgc acagaaaatt
    12481 cttcagatag gccaacgatt acttttgtat ggtaaaactg gcgcttgcga ctttgataca
    12541 cagcttctct taaaattttc tcgtatttgc tttgttcctt gtctaagtcc tcaaagatcc
    12601 gccgagcact tgctgttctt cccacaatgc ttccatcatt gaatactagt gtcctaacat
    12661 cagctggtaa atctagttct tcagcatgtg agattgttaa atctgtgatg atcgttcctg
    12721 gactcccttt tgctagttga taagctgtct tcctatcaaa aacttcatga acattattac
    12781 cgtaaaatgt cgtttctaca atcgatcgaa tactggataa cagtggattt ccacttgtga
    12841 tttcgtttaa ttcgagactc tcttttgtac tcatggctcc tcctcattcc ctaaacaaaa
    12901 aaacgttttc tttattatac attattcgag tttttatttg tactgctgcg ctgtgggaag
    12961 caaacgagtt atttttcaca caaaaaaacc gagaacttga ataaacaagt ttctcggttt
    13021 catcattttc cttttatcgg cttagtacca gccgtttgct aaccagaatg ttttagcagc
    13081 atcccatgaa ccataacgag atgttacata ttgttctgct acacgttctt gattggcagc
    13141 tgaatagtcg ccgtttaagt atgaagcatc taattgataa cggccgatgt aacgtccgtt
    13201 agtagctgtg tatgaaccgc ctgattcttt ttgtgcgatc cattcttttg cagagcttga
    13261 agttggcgct gttgcagcag ctggggcagc aggagctact tcaactgttt caacaggtgc
    13321 aacgtatgta tgttcttgcg ttgctgtttc agttgtagat gctgtatttt ctgttgtttt
    13381 gttttctgtt gttgctgaag aatcatttga agttgggatt gttaattgtt caccaacata
    13441 aatgcggttg atgtttgcga tcgaattttt ttcagcaatt gcatcgatca ttgagttgtc
    13501 tcctgcaaat ttttgtgaga tcttagataa tgaatcacct gattttactg tataaacttc
    13561 atcagcgtga gcatttgtag ttcctacaaa cattgcagta gcaccagcag caaaagttgt
    13621 acctaaaaca attttcttaa atgaattcat attaaactcc tttttttctc cgttttttat
    13681 tctttcgacg ctattaactt tatcatttag aaatatttca taggtaaaag aaacatgaca
    13741 tttaatgaca aaataacgaa ttatttaata tttgtaacat ttaaacaaat atttttttga
    13801 tacaaaacca taaaagcctt gttgtataag caaagtaatc gtttttctat gtttttttat
    13861 ttttgaatga aaaacgcaga ttctgtaaca ttcactgcaa taatccataa aaaaaagcaa
    13921 aaaaattttt gatttttatc aaattctctt aaaaaaatgt aaaaaataca aaaaaaccag
    13981 taattgttac aacaattact ggacaaaaac tttgaataat gttaactatt tttttatttt
    14041 aactttctcc gcaataattt ctgggcatgt tccaattcgt ttataacgac ttcttctgtt
    14101 tctttagcta atatttcatc taatacatca agccattttt ccggttcttt tgttcctaga
    14161 cgtccaatag cccaagctgc tgttcctctg atgacagggc ggacatctgt taaacattct
    14221 gaaatctcag gcaatgccgt tcgatcacct agattagcta aagcaatcaa tgcgtttctc
    14281 tgcaaaggtt tcttcccacg ccatgaacca gctaaatgcc caaattgctg tttgaagtct
    14341 ttattagaca tcgaaagcat tggctttaat tttggatata tttcgtctac ttttggttcc
    14401 atctcttcat gaaaatgaaa atcttttcct tgattgtaag ggcagactaa ctggcagata
    14461 tcacatccat aaatgacatt tcgcattttt ttacgatatt cttccggcat catccctttt
    14521 gtctgagttt gataagacaa acatcgctgg gcattcattc ttccgtcacc aagcaatgct
    14581 tttgtcggac aagcagtcac gcagcgcata cagtcaccgc aaccaaattc tcctggttta
    14641 tcaggctcaa atacaatatt agtaacgatc tcaccaagat agacaaatga accgaactct
    14701 tcagtgatca acaatccgtt tcgtccaata aatcctaaac cagctcgctg tgctacagcg
    14761 acatcgatca gttctccagt atctacttgc ggagctagac gccattcctc ttctttttgc
    14821 catttttctg ctcttgtttg gatgaattcg atcaatcggt tcaaacggtc tctgagtata
    14881 tcatggtaat cgatccccca agaagcacga gcaaactgcc cgcgtttttc atccctaggc
    14941 acttcttcat ggatcctagt aggatacgca agtgcaatgg aaatgatcga tttcggctga
    15001 tcaaatgtca tttctggata aagacgctca tcgatatttg gatgttcaaa gccagaagta
    15061 tggccagctg ctttttgttc ttccaacgat tcttttaatg aatcaaaagg ttccgccgtg
    15121 gtgaacccga ttttgtcaat tcctaatcgt ttgctttctt gtatgatttc ttcttttaat
    15181 gtcatggtct tcctcgcttg ctgttaacgg ctgtataacc cataatatag tttttctttt
    15241 ttcagctgga caaactggat ctgctgatcg gcaatcttct tttcttccat ccattttttt
    15301 cttgcagcaa atacttccca ataatttgtt gaaattaatt ttcttttgtt atctaatgca
    15361 ttaaaaaaat tcaatgaaac tggttttgta cttaacagtt catggagcct tacctcttcc
    15421 catggcgaaa actcagacag atcgcgggaa tacaatactt cttcatagag atttttttgc
    15481 tgtgtgtacg tgaatgtttg cgggattttc ccaggcctag caatttctac tagacgatta
    15541 taaaacatat tcattaattt gctgttggca ataaattgac ttttataata ggccgactgg
    15601 atctttttga ttactccttc tgctttgatg actgaaaata atgcaggcca tgctttgacg
    15661 gttatttcgt cgttttgttt aactaatacg ccaggtgtga agccgactgg agaaaggaac
    15721 acaaaataac cgataccggt attttcatct aaaggctgaa attcatcaaa caaccatttg
    15781 cctatgtaca gctcttcatc tggttctgtt gtctcatttt gatttgctat gaataattta
    15841 gctaattctg cttcaagaaa ttgaaggtct tgatccttgt cggtattttc ctgctggctg
    15901 atcaatcgaa agacttcttc tttaatcgta tatttatctt tacgtgtaaa attcatagcc
    15961 acaccttcct tccttactct aactataacg gaatttcttc aaaaaaggta tctgtacgac
    16021 gaaaaacagc ataatttttt ttcatatctc ttataaaaaa tgcaaggtct gctcctcaag
    16081 ctagaccttg cacttttcag ctttcttttg tttgtgacaa accttcatgg atcgtttcaa
    16141 tattccattt catcatctga tagtaagaat ctccttgttc tccttctttt gctatggaat
    16201 cagtgaacag tttatcatag attttcaaac ccgtttcttt tgctacccgt tccatgcttc
    16261 ttgagtctac actggtttcc acgaataaaa ccggtacttc ttttgctctt atctgatcaa
    16321 tgatcgcttt catttgatca ggcgtacctt gactttctgt attgatctcc catatataag
    16381 ctgctggcaa atcatatgct cgtgaaaaat acttgaatgc cccttcactt gttactaata
    16441 attttttgtt ctcttcgata gaagcaaaac tttccttcgc ttgtgtatct aattctttta
    16501 gtttttccac atatgcttct gcattttttt tatagagtgt cgcattttct gcatcttttt
    16561 cagagaatat acgtgcgatt tcctccacat attttattcc gttagatagg tctagccaag
    16621 catggggatc tgcttttgta tgttcttcac cgctagttaa ataaagaggt tctacatttt
    16681 tgctaactgc aaaataatct tttccttctt cttttttagc cgtttccatc aagttatcga
    16741 accagctgtt acctgtttca agattcaaac cgttgtataa aataacatct gcatcgcttg
    16801 cctttttgat gtcttctggt agtacttcat attcatgcgg atctgttccg actggtacga
    16861 tactgtggat atctatttga tctgttccga cctcttttgc catgtccgcc aagatcgaat
    16921 tggtagccac gactgacaac ttttctttac ttccttcatc cgcttggtca gaagcctgct
    16981 ttccgcaacc ggctaatacc aagaaactaa ctgccaagat taaaaagagt gatttttttg
    17041 ttttcatcta tctgcttcct ttctgaaata aaattccttt tgttggagaa aataaaaaag
    17101 caaggataaa aaatgcggta catgctaaca cgatcgtagc tcctgatggc caattgtaac
    17161 tataactgaa aaacacaccg acaactgcac ttagcatacc gattcctgca gctgtaatga
    17221 tcattttcag taaatggttc gttagcaagt aagccgtggc ggctggtgtg atcaacatgg
    17281 caatcaccaa gattgttcct actgtctgta aactgactac agccactaat gtcaaaaaga
    17341 acatcaaaag ataatggatc agcgatgtgt tcagcccata agcttttgcc attgtcggat
    17401 cgaatgatgt gatcttcaat tctttataaa agaaaaagac aaatatcaag acgattccac
    17461 taacgagtgc tgtcagcaac aaatcgcttt ccctaacagc taagacattc ccaaataaaa
    17521 tatgatacaa atctgtcgca ctctgggcat atgaaatcaa gataatccct aatgcaaaaa
    17581 aggagctaaa gacaattcca atcgctgtat catttttcaa tcggctccgc tgagtcacga
    17641 aaccaatcac tgctgctgtt agcatcccaa aagcagtggc tccaaagatg taactaaaac
    17701 caaacatata agaaatggct acacctggaa gaactgcgtg agagatagcg tctcccatca
    17761 aagacatccc tcgtaaaata ataaaagaac cgatgactcc agaagcaagt cctacaagca
    17821 tggaagtgac caaagcgtat tgcaggaatt gataattcat taatccatca ataaaggatt
    17881 ggatcatttt ttctctcctc cttttactaa atgtctgatg atctcaccat aggccaattg
    17941 aaggttcttc gatgtaaaga cttcttgtac tggaccggaa gcgatcagct gtttatttaa
    18001 aagaataacc tcatcgaaat attcttctac tttatgaaga tcatgatgga cgatcaaaat
    18061 cgtttttcct gaattcttca attcctgcaa gatgtcaaag atctttcgtt cacttaacgc
    18121 atcaatccct acgaatggtt catctaaaaa gatccattct gctccttggg ctagagctct
    18181 tgcaataaaa actctctgaa gctgtccacc cgatagttcg ctgatctgtc ttttggcata
    18241 ttcttccatc cctacttttt tcaaagcttg tcttgcacgt tctttttctt ttttcccagg
    18301 tctttgtcca attcgtaaag atggatatgt tcctaaaagt acaacgccta atacatcaat
    18361 tggaaaggaa agatccaatt cacttcgctg ttccacgtag gctatttttt tacggactga
    18421 gtttaccgac tgtccagaaa aggtgacttt accagtagta gctggtatca actctagcaa
    18481 agatttcata aatgttgatt ttcctgcacc gtttggtccg ataataccgg taattttttg
    18541 tggatttatc gtcaagttga tagttttcaa aacagttttc ccttgataag cggctgaaag
    18601 gtcttgaatt acaatggccg tacgttccaa atcttacact cctttttagg tttacctaac
    18661 tttttattta aatatagttt aactaaaatg gaaagcattt tcaagtagaa tgatgaaaaa
    18721 gtttttttcg aattttcctc gcattttatg aaaattttct gtagaatcac atcaagagtt
    18781 caataaggct aacctaataa tgaaaaacat gtgttgaaac gtttgattat tttttcttgt
    18841 tgactcaaca acgaaaggag tttttgtatg gaagaagaaa agttaactcg aagtcttagt
    18901 gcccgtcata tccaaatgat cgcactaggt ggaacgatcg gtgtcggtct gtttatgggt
    18961 gcctcctcaa ccatacgttg gacagggccc tcagtgatgg gagcctatgc gattgcagga
    19021 ttctttttgt atttgattat gcgtgcttta ggcgaaatgc tttacgttga tccttcgact
    19081 ggttcttttg caaattacgc gagcgagtac atccatcctg tggctggcta cttgaccgct
    19141 tggagtaata tttttcaata tattgtcgta ggaataagtg aagtgattgc tgtaggatct
    19201 tatatgaatt attggtggcc tgatttgccg gcaattattc ccggaatcat tgtcgtcttg
    19261 tttcttatgc tggcaaatct tatttctgtc aaggcgtttg gcgaattaga attttggttt
    19321 tcgatcatca aagtcataac gatcattttg atgatcattg caggtcttgg ggtaatctta
    19381 tttggatttg gtaatcatgg tcaggcagta gggatttcaa atctttggaa aaatggtgga
    19441 ttcttcactg gaggcgtcaa aggatttttc tttgcattat cgatcgttgt tgcttcctat
    19501 caaggaatcg aactgatcgg aatgactgct ggtgaagcag agaatcctaa aaagacgatc
    19561 attgaagctg tccagtccac tatcggtcga atcttgattt tttatattgg tgctattttt
    19621 atcattgtga gtatctatcc gtggaatcaa ttgagtgaga ttggttcacc ttttgtacaa
    19681 actttttcta aagtcggcat tacatttgct gcaggactga ttaattttgt cgtgattaca
    19741 gcggcattat caggttgcaa ttcagggatt ttcagcgcaa gtcgtatggt ctatacactt
    19801 gccataaatg gaaagatgtc taaaaaattt ctgaaactat ctcgtcacgg tgcgcctttt
    19861 tatcctgtcg tagcaatctc aggtggaatc ttaattggtc tgatcttaaa ttatcttcta
    19921 ccaatgttca tcaaccagtc agaaaatatt ttcgttttcg tatacagctc aagtattttg
    19981 cctggtatgg ttccttgggt agtgattttg atcagcgaga ttcgcttcaa aaagaagcac
    20041 gcagataaaa tgcatgatca tccttttaaa atgccactat ctccttatac gaattatgtg
    20101 acacttgctt ttttagctat cgtattgatt ttcatgttta tcaatcctga aacgagaatc
    20161 tcattgatta ttggactcgt tttcttgctt tacatgacga tccattactt tgtacgcgaa
    20221 aaaagaaccg gaaaataaaa aaagaacgaa gcttcacctt agtggaagct tcgttctttt
    20281 ttattgcttt tatcaattca attttagcgt ctcaccggga gtcaggaaat aattctccat
    20341 gctcatccca ttcagcgaca agatcgtttc tactggtacg ccagttcgtt cggctatttg
    20401 atttggacct tctccttcca aaactacggt tgtcgcagcc tgtgaagtcg cgctgttggt
    20461 attagtttca gaggaatctg ttgtgtcttg tgttgttcga gcttgcattt cagaagcttc
    20521 taccgactct gctgtctcag aatcagaaga tgttgtgtct tgtgtgcttg ttgactctgc
    20581 ttgaagagag gcgctagtct gcaaataaat atcggaaatc ttagcgacat gattcgtata
    20641 attagccgat aattgattga tcaaagtatc taagtctaca ccgtctttat tttctgctgc
    20701 ctgatacgct gcacgtaatt ttctgatccc ttcgtcggag attgcttgaa gctcacgtgc
    20761 tttttggtta gcaactgctg ataaaccagc tattccctct tgatttccct gaatttctgt
    20821 ctggtattct tcaatcaatg caggcgtctt ttcttcgatc tttttcgaat aatcatttac
    20881 gattgcttga agttgctctg gcaaaccatt tgctgttttt tcttcagaag tttgtgtctc
    20941 ttttgtttta gttgatgact cacttgtttg tttcgtctct gttttctttg tcgttgtttc
    21001 cgttatggtc gtacttttcg cttctgacga attttttcca tgaaaatata gacccgcaac
    21061 tcctcctgct atgatcaaaa acacggcaac tgctgcccca atcatgatca tccgcttacg
    21121 agctgcctgt ttttgctgtc ttcttcgggt catccgaaca ttttctcccc actcattttc
    21181 atcatacatt tttttcacca ccaattttta tgtataatag cgttatcaaa cgcttcatat
    21241 tcttattgta gcaaataatt gtcgctgtgc acatttttat ctttgttatt tataagaaaa
    21301 tttcaccagt tgagatgatt ccaagattgt ccgtcgattt ctccttgcag ctgcattgtt
    21361 cgttcgtcta tctcagccat cagtttcttt atttcatata agagcgcacg gtcttgatat
    21421 tctgattttt cgcttaattc atctaattgt tggatcagcc aatcatttgt cataatgaag
    21481 ttcctttcat ttcttgcagt gtaaaggtca agtcttcgat ctgctctttt gcctcagtca
    21541 gtgcttctac gatatatgct tgtttttcag attcttcttc ctgcattttc tgattttcct
    21601 gtactctttg aataaaccag tcaatccgct gagtcagcga tggtttgcct tccaatgaga
    21661 gatactgctg ataagcaatt agcttctccc aacgtttttc ctgggataaa tacataaatt
    21721 gtgctaaaac gaatggcgga agataggtca tatggaattt atttgcgatt cgctgatacg
    21781 gcctcaaaaa ttcggaaatc gtatattctt cttttcctcc agcttggtat tctttttccg
    21841 ccacacctgt tgtcaatacg attcctagtt ctttcccttt taatacatgt gctgctcgtt
    21901 ccaagacatt atcttgccaa atttttaaat gagcaggaga actataccag taaaacggaa
    21961 actggaaaat gatccgatca tgctgggcca agagctgtag ttcagtctct atatctattt
    22021 ttccgtctgg ataacttgat tccaaatgat gccaagtagc tggtaaacca gcagaagctt
    22081 ctttgaaaaa ttgctgattt aaagattctt cgatcgttgg atggctaatg atgataagtg
    22141 tacgcatccc ttttcctcct ttttagtctt cgctttacag tgtagcataa ccgccacaag
    22201 tttcttttgc cgatgcttgt cattttaacc agactaatca ggttgtccaa gaaactgcgg
    22261 tttgtttttc caactgtttt ttcaaggtat ccaaagcaat attcttaaaa ttttcgtttt
    22321 tcatacgttt acgatagttc gtagccgttt ctccggtact ctttttgaac attcggctaa
    22381 atacgactag actattgaac cctacttgat aggcaatact ttccacggat tcatccgtca
    22441 gagaaagaag aaacgcccct cgattgatcc gtaccccgat caaatattcc ttgatactgg
    22501 tacccatatg atttttgaat actcgcgaca aatggctccg attgaccgct aaatgctggg
    22561 cgatttcctc aacagacaat ttttcagcat aatgtgcctc gatgtatttg actgtttcaa
    22621 cagctaaaga ggaatgaacc ttttgtacac caccgctgac ctttccgcca tcttttaaaa
    22681 ggactgataa aaaacggtag gttaatgctg ttaactgtaa ttcattttcc aaggtttctt
    22741 ccgtatcttt catgcattct aaaataatgt cgatatattt ttgtatgtca gaagagacca
    22801 tcgtgtattg gtcttctctg aaaagcgact gccgaatgat ttcttctgca gcactcccac
    22861 caaatgaaag ccagcaatac atccacggat cttctccatc tgataagtaa aacgtcgagt
    22921 ctcctggacg aatcaagaac agatcacctt ttttcaattg gaattgttga tttttcacat
    22981 gataagatcc tttgccttcc atcacgacat gcaggatgta atcattgcga atagcaggac
    23041 caaaagaatg aaacggcatc gttttagaga aaccacaaaa atcaaaatac aattcttttt
    23101 ctacttgttg atgttccttt tttaaaaaaa tcgattgctc cacgtctatc cctccttact
    23161 cctttagtat aatgcaacaa aaagttaaat aaaagtcaca gaaatatatt aaaaaggaag
    23221 cgatttcttg ttaagtttaa gtcattaaaa gatctttgtt attcgttgta accggtttac
    23281 ttagtattat atattgaaca ggagaggaat acagattatg aaaagcaaaa tgatccaacg
    23341 tgtgtcctac gctttaggcg cttttggaca cgacacattc tatgttacat taagcactta
    23401 ttttatggta ttcgtgacaa gccagatgtt cgcaggaaca gataaggcaa cgaatgcaaa
    23461 aatgattgct acagttacaa gcttagttgt aggtatccgc ttagttgaaa ttatttttga
    23521 tcctttgatt ggtggcatca tcgataatac tcgtacaaaa tacggtaaat tcaaaccatg
    23581 gttagttatc ggcggcgttg ttagttccgt actcttggta gcaatcttta ccaatttctt
    23641 tggcttagct actcataata cgactttatt tatcatatta tttgcaatcg ttttcatctt
    23701 attggatagt ttttattcat ttaaggatat cgctttttgg tcaatgattc cagctttgag
    23761 tacggaaaca aaggaacgtg aaaaatttgc tacgtttgct cgtttcggct cttctctagg
    23821 tgcaaatggt actactctag tcgttgtccc tatcgttact ttctttacct atcttgttac
    23881 tcataaacat attgaaggcg cttcaggctg gttctggttc ggtgtgattg ctgctgttat
    23941 ttcaggcggt actgcgatta tcactgcctt gggcacaaaa gaagtcgata ctgcgatccg
    24001 tcagcaaaat gaaaaagctg ggatcaaaga tgtgttcaaa gcagttgctc aaaatgatca
    24061 gttgatgtgg cttgcccttg cttatatcgc ttatgcgatt gccaatgcag caacaactgc
    24121 cgtattattc tacttcttca aatatgtact tggccaacca actgaatttt ggatcgttgg
    24181 tctagttgct gccttacttg gtttagtagc tgttccacta ttcccaatgc ttacacgcgt
    24241 catctctcgt cgctacgttt accttggagg gatcgcaatg atgatttctt cttacttttt
    24301 ctttacgatc gcaggttcaa gtttgccaat cgttatcatc gggctagtat tgttctactt
    24361 ccctcaacaa ttgattttct tatccgcttt gatgacgatc acagactctg ttgaatatgg
    24421 tcaatggaaa aatggtatcc gtaacgaagc tgttactcta tcaattcgcc ctttactaga
    24481 taaaatcgct ggtgctttgt ctaacgggat cgtagggttt gtcgctatcg cttctgggat
    24541 gactggtaat gctactgctg ctgatatcac tgctcatggc gtaacgactt tcaaagctta
    24601 tgcattctat gctcctgctg ctttgatgat tttctccgct attatttttg cttggaagat
    24661 tacattgaca gagaaaaaac atgcacaaat cgttgagaaa ttggaaaaca aattggctcc
    24721 agctgataca atggaagatt aacttcacga tgcagtagtt ccttcccatt aagaaaggat
    24781 cttgaaaatg aagattacag aaaaagattt tggccaaggt tatcacttga taaccttgga
    24841 gaacaaaaac aagctagcac tttctatctc tgatttaggt gctagaatcg tcagtttaaa
    24901 atcaaatgat cgagaattag ttttgggatt tgacaccgct gaagaatata tcgaaaaaga
    24961 tccgtatatc ggtgcttcta tcggtcgcac cgcagggaga atcgaaaatg gccgcttttc
    25021 tctcaatgga aaaacttacc aattagccac agatccaaaa actggacata atctgcatgg
    25081 tggtgctcct ggatttgaat taaaaaaatg gtcttatgtc attttgaatg gcgaaaatga
    25141 agcttctgtc atttttacta ctacaagtcc cgacggagaa catggttttc caggtacaat
    25201 ggatgtggaa atccgctata ctttgacaaa agataatatc tggcgagtga cctctcgcgg
    25261 caccagtgac caagatactt tattcaatcc gacaaatcat gtttacttca acttgacggg
    25321 agatgcttct cagtcaatcg atcaacatga gctttggctc aatagcgaag cttatgctcc
    25381 tttgcgaaca gatagtattc ctataggggt aaaagaaaat gctgcaggaa gtgctttcga
    25441 ttttcaaata cctaaaaaat tagcgtccgt ttttgctagt gatttggatc aaaaaaatct
    25501 tgttgacgga atcgatcatc catttttctt aaaagaaacc ggcttgggta aagaagccgc
    25561 tcgcttgact agccccgata aaagaatcca agtagatatt gctacagatg cttctagtgt
    25621 ggttattttc actgcaaatt tcggcacaga aacaccagaa atgcgcaacc gaaaactggc
    25681 acatcacggt ggcatcacat ttgagactca aacagctcca ggtgcagagc gcttctcagc
    25741 atttggttcg attcatttaa aagccggatc agtatttgaa acagtaacag aattcaaaat
    25801 caaaacaaga aaggaataac tcatcatgga aatcaaaaga agcttaaaca caaaatttgc
    25861 cgaaacattc ggacctaaaa aaactacaca atacttctca ccaggacgca tcaatttgat
    25921 tggtgaacac acagattaca atggaggcca tgtcttcccc gcttccatca cttatggaac
    25981 gcaaggtgta gcagcacctc gagacgataa taaaatatta gtttattcca caaactttga
    26041 cgatatcggt gtcattagtt ttactttaga tgagctagaa tatgataaaa aagcaaactg
    26101 ggcaaattac gtaaaaggaa tggtccttaa attaaaagaa gctggacact caatcgatca
    26161 tgggtttgag ttattagtgg aaggaacaat ccctaatggt gcaggacttt ccagtagcgc
    26221 ttcattagaa ttattagtag gtgttgtatt agaagatctg tttgatttaa aaacagatcg
    26281 cttggaacta gtccaaacag gtaaaaaagt agaaaatgaa ttcatcggtg tcaattcagg
    26341 tatcatggat cagtttgcca tcggcttcgg tgagctagat catgcaatct tattagatac
    26401 aaatacgctg aaatacgaaa tggttccagt gaaactagat ggttatgcaa tcgtcatcat
    26461 gaatacgaat aagcggcggg aattggcaga ttcaaaatac aatgaacgcc gtagcgaatg
    26521 cgaagaagcc ttggcacgtt tgcaaaaaac tttgcctatc tcttctttag gagacttaga
    26581 tgaagaagaa tttgaatcaa ctattgacca aatcggcgat gatacattga tacgcagagc
    26641 tcgccatgcc gtttctgaaa atcagcggac tttgaaggca aaagctgaat tagaagctgg
    26701 gaacttagaa gcatttggtc aactgttaaa tgattctcac cactctcttc gctatgatta
    26761 tgaagtaact gggatcgaat tagatacact agtagatgct gctcaaaaac aagaaggtgt
    26821 cttaggtgca cgaatgacag gagctggatt tggtggctgt gcgattgctc ttgtcaaaga
    26881 agaaaatata cctgccttca aaaacaatgt ctatgatgaa tacatgaaag ttgtcggcta
    26941 tgctccggaa ttttatgttg cacacattgg aaatgggaca acaaagatcg atccagaagc
    27001 tgttcatgtt tgataaaaaa atcaacgatc tcagctaaaa acaaatagat aaaaataacc
    27061 tctcagcaat ttccaaatgt gagccttttg ctcgtatttg aaaattgctg ttttttattg
    27121 acacggaaag agaacgtgtg ttctaattgt gttgaagagg tgattgttat gtgggcgaat
    27181 caaaatccgg tatttgttta tgaaaaagaa ccttcaagag atattttatg tattgactgc
    27241 aaatcttttt acgcctccgt agagtgcgtt gaaaaggatc ttgatcccct tacaactaaa
    27301 ttagtagtca tgtcctatcc gtccgattcg acagaaacac ggggtagcgg attaatcttg
    27361 gcttccagtc ctgcagcaaa aaaagcttac ggcatcacca atatcagccg tgccagagat
    27421 cttcccttcc cctatccaag tgatttatat attgttgccc caaggatggc ctattatatg
    27481 gaaaagaaca gagaaatcaa ttctatctat aaaaaatatg cagacgaagc gaaccatcat
    27541 gtctattccg tcgatgaaag cttccttgat gtaacaaata gcatggcact ctttggtaaa
    27601 acaaatgcgt acgacatggc taaactgatc cataaagatg tgtttgagca gaccggaatc
    27661 tacacgacta taggaattgg cgataacccg ctacttgcta aactagcctt agacaatggc
    27721 tcaaaagacg gaaagcaaat gtttgctgaa tggcgttatg aagatgtacc agaaaagtta
    27781 tggaaaatct cagatatcac tgacttttgg ggaatcggca aacgaacagc aaaacgtctg
    27841 aaacaaatgg gtatacattc tgtttatgat ttagctcacg cggattatta tcacttgaaa
    27901 agcaagctag gggtcatcgg tactcagctt tatgcccatg cttggggcat cgatcgaagt
    27961 tttttgggac aagaatatac accaaaatca aaaagtatcg gaaatagtca aatcctagat
    28021 agagattata caagacgaga agaaatcgag atcgttatca aagaaatggc cgaccaagtc
    28081 ggcactcgct tgagacgatc tgcagcaaaa acacaagtag tcagcttatg ggtaggatac
    28141 tctttaggct atcttgatcc aaatggcaag accgggtttc accagcaaat gaaaatcgaa
    28201 gcgacaaatg ctagcagaga tcttgcagat gctttattga ccattttcca ccagcattac
    28261 cactaccagg atattcggcg tgtaggcgtg aactgttccg acctcgtata cgcgacaggt
    28321 ttacagctta acctttttga agatcctgaa aagcagataa atgaagcgaa agttgatttt
    28381 gttgtcgata cgattagaaa aaaatacggc tttcaatcaa tcatccatgc gcatagtctc
    28441 ttgcaaggag gacgcgccat cgcacgtagt tctctcgttg gcgggcatgc aggtggtatg
    28501 agtggattag aaggtggaaa agatgagacg gacagcaaaa aaattcattg actataacga
    28561 gtatcgagat cggccatttg gattaaaatg ggggactgct tttgcgatgg aagaactagt
    28621 caacggtatc aaaaaaatga agaagaagca cgcaaacagc ctcttcctaa acaacaaatg
    28681 acattgcaag aaatcgatag tatactgaca gaagcctttt tatctcgtaa agcagtaatc
    28741 attcagctca atttaaaaga tgaatttgga agactcttgg atgaggtcga agggcacttc
    28801 attggtgaag cctatgaaga ttactttatg attgaccatc agccgatatt gtgggaagac
    28861 gtacggtata ttgagttaaa ggaaacaaaa aaatggtttg tgacatgaga ggttgtgaca
    28921 gaagtgatca acttcaaaaa ataagaaagg atttttcgaa aattgcttct taaatttttg
    28981 tggaattttg gcttattttt cgaagaagca acttctggaa caacgtatat tagatatcag
    29041 aggttgtgaa aaagtcgctt agttttgagt aacaagccaa ggcgctgatt tcacaacctc
    29101 tgtctatcta tccatctatt cggtaagatt catgcatgac gtcttcttta tttagtatgt
    29161 agagcccttt ttcttctgac cattcaacat aaatcagctg ttttacatct gcatagcctt
    29221 ctttcttcaa gttctccata agccatgctt cagtcaattg attggactga agcactttat
    29281 gctgaatttg cccttcatct acaagcagga tcgataaagc tttgtcttca tctgattttt
    29341 tcaatacact catttgaccg gcattctcta ggataacatg tttggcattt tcaagaaaaa
    29401 agcattcttg ctgtcttaag attgctcgca actgctccat ctcgatatgg ttatttgtca
    29461 actctgtcat gttgatgacg ccgtctttga ttagaacaga tggctcgcct ttgacccaac
    29521 gattgacctt ttcatttttt tggacgatac gttcgatacc ataaatcatt actgcccaaa
    29581 gcgcaatggc aaataggaca tgccctacat ggacattgtc atcataggtt gattcttcta
    29641 agatcccacc taaaacaagc gtatagacta aatcaaacgg tgtgatttct gacatggatt
    29701 tcttcccaag caaacgtacc actaataata aaccgatcaa cccgaacacc agtttcattg
    29761 taattgaaaa aatcattttt tctcccccta actatctgtt aattaaatta tatatgcttt
    29821 ctcacgaaca cgcatggaat atacaaggaa gaaatgtatc cttttactaa aaaaaggact
    29881 gtgacactgt agtgaccccc aaaagttaga cttttttgga gtggagaaat ccactccttt
    29941 tttttatgct ctaaaatgat atttttacac aaccgttacg gttgttttac gaaattggac
    30001 g
//
LOCUS       DAZKLD010000016        29111 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.016, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000016 DAZKLD010000000
VERSION     DAZKLD010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29111)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29111)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29111
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.016"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1031)
                     /locus_tag="ACG8KR_000638"
     CDS             complement(<1..1031)
                     /locus_tag="ACG8KR_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFQ0200038.1"
                     /translation="MKSNEKTPLTTSLILLLSITCGVVVANMYYIQPIGTKVAASLSV
                     STSAVGILTMLTQLGYALGLLFLVPLGDVVDRPKLIIRMVALSAVSLLAAFFAPSFTL
                     FACASFLIGLLSIVPQIIIPYGAVLAGPKARGKVMGTLLSGLLVGILLSRTVSGIVAS
                     LFSWRMIYLFALCAVGILTLLLYWKMPRSVQQSSTAVSYLDSLKSLPHLVASQRLLRE
                     AAVSGFFMFGTFSIFWSTLIFYISSPAYNWGTFEAGILAIFGLSGAVAAPIVGRLADS
                     YSERKIVYMGLVMQTISFIALLVGGSHLAILLLAIILLDVGNQFGQVANQTRVQGLGE
                     AASNRNNTV"
     gene            complement(1113..1802)
                     /locus_tag="ACG8KR_000639"
     CDS             complement(1113..1802)
                     /locus_tag="ACG8KR_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFQ0200039.1"
                     /translation="MKKKIIRLMLISLGIILILVVGYRLYLEYQTDIQLFLNPRASKA
                     ELLAHIRSHGTFASILLIVLTAVMCAVPGIPTSVVGVIAGLSFGPLTGAIINILGNAL
                     GNITAIFLMHRLKFLDKKTETNHWVQAIRQMKHPKIGVMLGYMIPVIPSSMVNFAADA
                     MKLPLQQIILSIVIGVIPSSVLYACGGEALFHGYNKTAVTLVASVTVLVGLVVFIYKD
                     RKKKQGKQKHK"
     gene            complement(1908..2276)
                     /gene="rplL"
                     /locus_tag="ACG8KR_000640"
     CDS             complement(1908..2276)
                     /gene="rplL"
                     /locus_tag="ACG8KR_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HFQ0200040.1"
                     /translation="MALNIENIVAELKEATILELNDLVKAIEEEFGVSAAAPVAVAAA
                     GGAAAAEEQTEFTVELTAAGDQKVKVIKAVREATGLGLKEAKAVVDGAPAPVKEGVSK
                     EEAEELKAKLEEVGASVTVK"
     gene            complement(2329..2832)
                     /gene="rplJ"
                     /locus_tag="ACG8KR_000641"
     CDS             complement(2329..2832)
                     /gene="rplJ"
                     /locus_tag="ACG8KR_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HFQ0200041.1"
                     /translation="MSEAIIAKKAAIVDEVSEKFTAAASVVVVDYRGLTVDEVTRLRK
                     QLRDANVEMKVIKNSILSRAAKKAGLEGMDEVFTGPTAVAFSNEDVVAPAKIMDEFAK
                     EAKALEIKGGIIEGKVSSLEEITALAKLPNREGLLSMLLSVLQAPVRNVAYAVKAVAD
                     KEDGDAA"
     misc_feature    complement(3036..3163)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            complement(3242..3931)
                     /gene="rplA"
                     /locus_tag="ACG8KR_000642"
     CDS             complement(3242..3931)
                     /gene="rplA"
                     /locus_tag="ACG8KR_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HFQ0200042.1"
                     /translation="MAKKSKKMQDALKKVDSSKVYPVAEAVALAKETNIAKFDATVEV
                     AYRLNVDPKKADQQIRGAVVLPNGTGKTQTVLVFAKGDKAKEAEAAGADFVGDDDMVQ
                     KIQNGWFDFDVVVATPDMMATVGRLGRVLGPKGLMPNPKTGTVTMDVTKAVNEVKAGK
                     VTYRVDKAGNIHVPIGKVSFDDEKLVENFAAIHDVLLKAKPSAAKGQYMKNVTITTTF
                     GPGIHVDQASF"
     gene            complement(4035..4457)
                     /gene="rplK"
                     /locus_tag="ACG8KR_000643"
     CDS             complement(4035..4457)
                     /gene="rplK"
                     /locus_tag="ACG8KR_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HFQ0200043.1"
                     /translation="MAKKVEKIVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGLIIPVVISVYEDRSFTFVTKTPPAAVLLKKAAKIEKGSGEPNKNKVAKVSS
                     DQVREIAELKMEDLNAANVEAAMRMVEGTARSMGITVE"
     gene            4800..5468
                     /gene="sdaAB"
                     /locus_tag="ACG8KR_000644"
     CDS             4800..5468
                     /gene="sdaAB"
                     /locus_tag="ACG8KR_000644"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HFQ0200044.1"
                     /translation="MKDLRYRSVFDIIGPVMIGPSSSHTAGAARIGKIIRSIFGEQPD
                     SVDIYLYESFAKTYRGHGTDIALVGGLLGMEPDDERLADSLKLAYEAGMEVLFVPKSE
                     KAEHPNSVKMLLKKGTRKLSVTGISIGGGNIQISELNGFKISLSMGTPTLIVVHQDVP
                     GMIAKVTSILSETNTNIGTMTVTRESKGENAIMIIEVDDPQVEETVKRLKQLPNIDSV
                     NYFD"
     gene            5481..6353
                     /gene="sdaAA"
                     /locus_tag="ACG8KR_000645"
     CDS             5481..6353
                     /gene="sdaAA"
                     /locus_tag="ACG8KR_000645"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HFQ0200045.1"
                     /translation="MFLSIEELVDQAKAYPSVAELMIAVEMEMSGRSRSHIIEIMEKN
                     LSVMEQSINEGKEGVTSVTGITGGDAKKLDDYLNEADFLSGETILKAVRNAVAVNEVN
                     AKMGLICATPTAGSAGVVPGVLMAVRDRLDLTHEQQLEFLFTAGAFGLVIANNASISG
                     AEGGCQAEVGSASAMAAAALVCAKNGTPHQAAQAVAITLKNMMGLICDPVAGLVEVPC
                     VKRNALGSSQAFISADMALAGIESVIPPDEVVAAMYQVGRQMPSIFKETAEGGLAVTP
                     TAKRLAEEILNKRA"
     gene            complement(6444..7544)
                     /locus_tag="ACG8KR_000646"
                     /pseudo
     CDS             complement(6444..7544)
                     /locus_tag="ACG8KR_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324221.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
     gene            complement(7636..8523)
                     /locus_tag="ACG8KR_000647"
     CDS             complement(7636..8523)
                     /locus_tag="ACG8KR_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFQ0200046.1"
                     /translation="MTEPIKNLCKRATAAALVLISLFALSGCQTTKINTTEVSDKEKT
                     AESSKVIEKTTIPTLFFHGYSGTENSFKGMLRRLEKNQQAVKELVLTVGTDGQIQATG
                     DLSGKADNPMVQVLFEDNKNNEWNQAEWIKTCLLYLKENYGIDKVNIVGHSMGGVSAL
                     RYLGTYGQDTSLPQVQTLTAIGAPFNDFVDDSAQSLTDELAKGPAVVSNRYQDYQQMI
                     GNIPITTRFFLIAGQLDETDLSDGTVPLNSALAVYALLKQRGNEIEEKVVTGENASHS
                     MLHENQEVDQLVSRFIWKE"
     gene            complement(8600..9145)
                     /gene="nusG"
                     /locus_tag="ACG8KR_000648"
     CDS             complement(8600..9145)
                     /gene="nusG"
                     /locus_tag="ACG8KR_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HFQ0200047.1"
                     /translation="MESFEKNWYVLHTYSGYENKVKANIESRAQSMKMADFIFRVVVP
                     EEKETEVKNGKEKEIVHKTFPGYVLVEMIMTDASWYVVRNTPGVTGFVGSHGAGSKPA
                     PLLPEEVNHILRSIGMSLRQNDLEVEVGEVVKIIEGAFAGLEGQVTEVDGEKEKLKVN
                     IDMFGRETSTELDFDQVDKID"
     gene            complement(9246..9416)
                     /gene="secE"
                     /locus_tag="ACG8KR_000649"
     CDS             complement(9246..9416)
                     /gene="secE"
                     /locus_tag="ACG8KR_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HFQ0200048.1"
                     /translation="MKFLKSVKDEIKQVTWPSRKQLRKDTLVVIETSIIFGLMFYVMD
                     TAIQSLFGWILK"
     gene            complement(9440..9592)
                     /gene="rpmG"
                     /locus_tag="ACG8KR_000650"
     CDS             complement(9440..9592)
                     /gene="rpmG"
                     /locus_tag="ACG8KR_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFQ0200049.1"
                     /translation="MAKKKAALACSVCGSRNYTKSVSEGKSGERLEINKFCKYCNQYT
                     LHKETK"
     gene            9754..10398
                     /gene="cbpA"
                     /locus_tag="ACG8KR_000651"
     CDS             9754..10398
                     /gene="cbpA"
                     /locus_tag="ACG8KR_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-AMP binding protein CbpA"
                     /protein_id="HFQ0200050.1"
                     /translation="MLLKTLVYKKKDLTTVTETSTLEEALKILETSGYRCVPILDSSG
                     NIFRGNIYKMHIYRHKANGGDMTLPVTHLLKNATKFIYLDTSFFKVFFTIKELPYIAV
                     LDSENHFYGILTHSTLLNMLSQSWSVEQGSYVLTIASTGKQGDLAAIAKIISKHSSIA
                     SCITLDIEKDEYIRRTLITLPANTEAKVCETIVNHLEKKNYKVVEIENLQNQEA"
     gene            complement(10454..10957)
                     /locus_tag="ACG8KR_000652"
     CDS             complement(10454..10957)
                     /locus_tag="ACG8KR_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HFQ0200051.1"
                     /translation="MQKTKSRQRLSVIVVNGIIMALYLALTILVAPVSSGPIQFRISE
                     SLNHLVVFNRKLLWGVLGGVIVYNAFFGFGIMDVVFGGGQTLLSLGLTALLQNKVKNV
                     KMRLVLNTIFFTISMGLIAYMLVPTGGQAFWTTYGTLALSEFIVMAVSAPLMYYLDRA
                     LDFEKRV"
     regulatory      complement(10970..11016)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            11554..12519
                     /locus_tag="ACG8KR_000653"
     CDS             11554..12519
                     /locus_tag="ACG8KR_000653"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFQ0200052.1"
                     /translation="MKFHYHILINPSAGSGNGYKVAERILPVLKNKHIDYTLHYSEYK
                     GHDAEIAETLAKETLIPWIEEEQKTIDTFPLLIIVGGDGTLHQVLDTFYQMEVEFPVA
                     YIPAGSGNDFARGAGLPKNPKKGLQLILAAQSPEKVHIMAYEEKISEKKGIAVNNFGI
                     GLDAAIVHATNHSSTKKRLNKYNLGSFSYLFSILRALFTQKGFPILVDAGGKQLSFSN
                     AFLCTATKHPFFGGGIAIVPTADASKPVIDFVVVERINFFKILWLILLLIQKKQLKSK
                     YFHHYTTNRLRIVSTTPQHGQEDGEEMEKRPFDITITTTKQLFWC"
     gene            complement(12618..13790)
                     /locus_tag="ACG8KR_000654"
     CDS             complement(12618..13790)
                     /locus_tag="ACG8KR_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0200053.1"
                     /translation="MTLYKTIWTLLKANKSNLLIGVIITVVATFFFAQNIDVENTSLQ
                     DAKIALISEEESPVVDGLRKYLNDKETVVTLDGTSQKEIDDALYFNRVNIILHLPADF
                     TEQVEVGKMATIKIQSRPDAFSKTVVTQQVNTFLQTLILFQEEDKTIDAAMEQTQMAL
                     SVTGEVELSAGYSQRMKKLLTGTTFNFLSYGLFLSIFSGFSVINLAFNRKEISKRNQS
                     APITKRNLNRKVTFSLISYSLLLFSGFLLLMVLLVIHTSWDITVWYHILNTFLFLLPI
                     ISFSACITSLVKNSETSGGIKDIFITGSCFIGGVFVPAEYLPEMVSKIAAFTPTYWFV
                     QNNNLISETLSYNQTFAESFWFNGCVLVAFAAVFSMIQFILGKERNYRWQPNWAKN"
     gene            complement(13777..14922)
                     /locus_tag="ACG8KR_000655"
     CDS             complement(13777..14922)
                     /locus_tag="ACG8KR_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0200054.1"
                     /translation="MFWHFYKYRTKILLRNKSMLFWTLAFPILLGLMFMAAFGEIDQG
                     GNLETINTGIVIKADQTALSENFTTVLEQVEVNDKPLFEVQELSEVEANEKLNADDLA
                     GFYLIDGSSVSLHVGQAGMSQTLMKNVMDQFLQRIGIISSKVAEVENVDLAPIMTVFE
                     QEVSFQEVHAGRNMSFKSFYFFTLIGMAILYGTMWGVRNVQDQQANQSSNGIRLSLIP
                     RKQSLVSIANLAASFTIVLTEIYIMLAVFRFIYQVDFGERWQWLMIVTALGSLCAILL
                     GTLVGNLIPKMNLVQKDSILISVTIAMSFFAGMMGSEQVKYWIDLHIPIVGQLNLVNL
                     ISESLYKLYFYTDLASFYTNLLWLIGFIFLFALGNAWMERGVRYDAL"
     gene            complement(14936..15868)
                     /locus_tag="ACG8KR_000656"
     CDS             complement(14936..15868)
                     /locus_tag="ACG8KR_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0200055.1"
                     /translation="MIIEIDKLVKRYGDFVALNHFDLQVQEGEVLGLLGPNGSGKSTA
                     INCMLSLLNYDKGTIKLFGTTMTPENYAVKAMIGVVPQDVAVFDELSVQENIDYFCGL
                     YVSEKEKRKALIEEVIQLVGLEKYVSFRPGELSGGLKRRLNIACGIAHKPRLIFLDEP
                     TVAVDPQSRNKILESIKELNRQGATIVYTTHYMEEVEILCDQIVIMDQGQVIAKGTKE
                     SLKDMVRTADRLTIDIPLLPENVLSAIQRTIASDGIAYKKGQLQVTTTELNTVLLPIL
                     TILQEEKISYGKIATKEASLNDVFLEITGKDLRD"
     gene            15985..17064
                     /locus_tag="ACG8KR_000657"
     CDS             15985..17064
                     /locus_tag="ACG8KR_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HFQ0200056.1"
                     /translation="MHKSRLLNETSFLLVISLLLLVSQGFVAMQVIYLLIAVLFTGVS
                     YLLPTKPNWIFQVILFLLCAFLPDLVLFLPATMRVIAPEESHLDYLFLLGFLLISTVG
                     NHPPIKVAFVVILLFFSFYLRKKDQRTTDLEKKYIVSKDTHWEKQQELSEQNKALALS
                     QETQLALQIVEERNRIARDIHDNVGHLLSSAILQIGALEMINQEPKLKEPLENLSATV
                     HTGMDRIRQSVHDLHETSISFQHSLEFLIADFPSSVTIEGNLFEGLNETQQNCFLMVI
                     KEALTNSIKHSHAKTVVLHFRTLPAFYRLQITNDGTSPQKNSAPGIGLKTMRQRVQEQ
                     KGQVHIFQENQQFQLTIILPREGHQ"
     gene            17061..17690
                     /locus_tag="ACG8KR_000658"
     CDS             17061..17690
                     /locus_tag="ACG8KR_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFQ0200057.1"
                     /translation="MITVVIIDDDPFVTKSLQTILESTKEIRVLGIGHCAKDALVLYE
                     THRPDVLLTDIRMPKQTGIDAAKEILAKFPKAILLLLTTFKDEEYIREAFSIGVKGYL
                     IKQNLQAIIPSVKSAYNGQAVFGNEIIETFTQMMKNKPTIDSHAYASFSERELAIIKE
                     VAAGKNNKEIADALYLSDGTVRNYISQLLEKLDLRDRTQLAIYFYQHLQ"
     gene            complement(17800..18027)
                     /locus_tag="ACG8KR_000659"
     CDS             complement(17800..18027)
                     /locus_tag="ACG8KR_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFQ0200058.1"
                     /translation="MAKETEDKLAKLAKESGNDFKKLDEMEKEFKLNESPDVERDDRE
                     ESKLEKTLDKAPIFPPNTPNNPTNPGSPANP"
     gene            complement(18130..20718)
                     /locus_tag="ACG8KR_000660"
     CDS             complement(18130..20718)
                     /locus_tag="ACG8KR_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0043139 - 5'-3' DNA helicase activity
                     [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HFQ0200059.1"
                     /translation="MQPEEMPYFVGTVAAIFFQNPSNFYKVLLVHISETTADYREKEI
                     VVTGSFGDIQENETYRFYGELVDHPKYGRQLKLERYEQEKPTTANGIVAYLSSEKFPG
                     IGKKTAEKIVELLGEDAIEKIIADPSVLKQISGLTKPKREMLAETIRLNHGMDQVIMG
                     LNRYGFGSQLAFSIYQAYKNESLDIIEENPYQLVEDIEGIGFKRADNLAEQIGIAADS
                     PRRIRAAILHQIFQHAVQTGDTYVPAETLLQQTIRILETSRPIEIQPDQVATVIIQLV
                     EEGKIQQEETNLYENSLYFSEWGIGNSIQRLLSRKKEIHYPEKEVQKNIRRIEKIFGI
                     QYGPSQEQAIAEAIRSPLFILTGGPGTGKTTVINGIVNLFAELNGLELDPSKYTQEIF
                     PILLAAPTGRAAKRMNETTGLPASTIHRLLGLNGREKTPSVSTKELEGGLLIVDEMSM
                     VDTWLANTLFKAIPTNMQVILVGDKDQLPSVGPGQVLHDLLEIEQIPKMELNQIYRQG
                     DGSSIIPLAHEIKEGRLPKDFTKNQKDRSYFRSDAYQIEPLISKIVEKAKNKGFRAQD
                     IQVLAPMYRGPAGIDALNKMMQEIFNPGKPGKKEVHWNDTVYRIGDKVLQLVNTPELN
                     VFNGDMGEITGIILAKDSEDKVDELVIQFDANEVTYKRNEWNKITLSYCCSIHKSQGS
                     EFKMVILPMVHQYQRMLQRNLLYTAVTRSKELLILLGEEQAYVTCVANESASRMTTLK
                     SRIMDNDKMNITLRTKLAAYEDSLVGNDPFEDGNYTKEPRVVKEKLPVEDDSGTNETK
                     NQVMETVEPDLFSSFVGEEKKEEVEPAVSKDFVLTAEVIDNQLVDPMIGMENSSPYDF
                     IPAKNA"
     gene            complement(20738..21397)
                     /locus_tag="ACG8KR_000661"
     CDS             complement(20738..21397)
                     /locus_tag="ACG8KR_000661"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFQ0200060.1"
                     /translation="MELYFTRHGKTQWNQERRFQGSNGDSPLLPQSYEEIKAFGKKVK
                     YVPFKAIYSSTAKRARDTAEGINKELARPVEIFYTDKLRELGLGTLEGQSIDEMYQKY
                     PDNLPNLRNHLDLYDPSPFHGETIESAITRIETVVADAVARHKDGPLLFVGHGASLTA
                     AIQWMTGKELSELREMGGLFNSSLTILETEEPCRLLPYELKVWNQTDFLGTETAPEPL
                     L"
     gene            complement(21446..21949)
                     /gene="trmL"
                     /locus_tag="ACG8KR_000662"
     CDS             complement(21446..21949)
                     /gene="trmL"
                     /locus_tag="ACG8KR_000662"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HFQ0200061.1"
                     /translation="MNHIVLFEPLIPANTGNIARTCAATNTPLHLIEPLGFSTDDKHL
                     KRAGLDYWNDVNITYHSNLEAFLTFLGNRKLHLISKFAHKVYSDENFADGEEHFFLFG
                     KETTGLPETFMRENEEKCLRIPMNDEHVRSLNLSNTAAMIVYEALRQQGFPNLELTHH
                     YENDKLD"
     gene            complement(22170..23036)
                     /locus_tag="ACG8KR_000663"
     CDS             complement(22170..23036)
                     /locus_tag="ACG8KR_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HFQ0200062.1"
                     /translation="MLKKIDRGRQFLAENHQLFRCPVCQEEVKKSDNGLICKNNHRFD
                     LSKKGTLYFLSHAVKTEYDQGMFVPRRRMIQSGMYQPVIETIAQHLPEEVSLLDVGCG
                     EGSFLNELSKLKKIQNAVGFDISKEGVYAATEQSAEAFWCVADLTNLPFADQTFNAVL
                     NIFSPSHYQEFQRVLSEDGEVLKVVPQENYLKELRQAFYPNQPEKQSYSNERVVAKFA
                     EAMKLTERQRVTYEFEIPEENQKDLLAMSPLEWQVEERIKADLYKNPLKKITIDIELL
                     KGTKRKRFSSSF"
     gene            23145..23786
                     /locus_tag="ACG8KR_000664"
     CDS             23145..23786
                     /locus_tag="ACG8KR_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HFQ0200063.1"
                     /translation="MIKEFCAENYTSIPLAIARGADRIELCDNLAVGGTTPSTGVIEE
                     TLSYAGEKDIPVMTIIRPRGGDFVYNDIELKIMHTDLIEAKKLGTDGVVFGCLTPSGW
                     LDEEALELLIETAEGLQITFHMAFDSIPEDRQFEAIDWLVEHGVDRILTHGGPADKTI
                     VENFPRLKELIAYTDGRLIILPGGGVTAENAAHVAKELNVSEVHGTKIVDLQL"
     gene            complement(23848..25209)
                     /gene="mgtE"
                     /locus_tag="ACG8KR_000665"
     CDS             complement(23848..25209)
                     /gene="mgtE"
                     /locus_tag="ACG8KR_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HFQ0200064.1"
                     /translation="MDENQELEERFAQLTQDLRENNIQAFRNSFLEMHIYEQGQFYQS
                     LTEENRQLIYSYLSPKELADMFDVIEEDNEHMKEYLDEMRPSYAADMLSEMYTDNAVD
                     LLNTLDKKQIAKYLSLMSADDASEIKELLHYEDETAGAIMTTEFVSIVANQTVRSAMY
                     VLKNEADVAETIYYIYVVNQEGQLVGVISLRDLIVNDDDAMISDLMSERVLSVHVGDD
                     QEDVAQTFRDYDFLALPVTDYDDHLLGIVTVDDIIDVIDDEAASDYSGLAGVNVEEIN
                     ENPVKAASRRLPWLITLLFLGMSTASLISHYEDLVSEASILAVFISLITGTAGNAGTQ
                     SLAVAVRRLAISDEKDHGFVRLVLAEVLTGVVTGAVTGITIFLIVGFWKHNFILGFVI
                     GMAMLFAITVANLAGSLIPMLMDRLGFDPAVASGPFITTLSDLTSVLIYFNIASLFMG
                     YFM"
     gene            complement(25227..26126)
                     /locus_tag="ACG8KR_000666"
     CDS             complement(25227..26126)
                     /locus_tag="ACG8KR_000666"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFQ0200065.1"
                     /translation="MQFTWDYEKDHSQQVKYFLKEKGISKGLLAKIKFQGGQIKVNDQ
                     VENVLFSLAKDDKVTIVIPAEGEHETVLLDETPIDIVYEDEHVLVVNKPAGVSSIPAQ
                     YHPNGTMANRVKAYYKKQGYENQVIHVVTRLDRDTSGLMLFAKHGFAHAKLDMQLREK
                     KFVKKYQALVSGRLSDLSAHGHIDLPIARDYSSLLKRMVSDEGRSAQTEYWIEKRLTE
                     IALVDIQLHTGRTHQIRVHFSAIGCALLGDDMYGGRMDLGIKRQALHCYDLRFYHPFT
                     EELLEFKQPLAADMEAVVRRFEE"
     gene            complement(26128..26925)
                     /locus_tag="ACG8KR_000667"
     CDS             complement(26128..26925)
                     /locus_tag="ACG8KR_000667"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HFQ0200066.1"
                     /translation="MKVAIVHNKEAKTIEVTERLNALLEQAGIQRDDHEPELVISVGG
                     DGTLLSAFHHYSHCLNDVRFLGVHTGHLGFYTDWRDYELEELVDSLRTNREQSVSYPL
                     LDVRISYLDETPDQHFLALNESTIKRANRTMVADVYIKNELFESFRGDGLTISTPTGS
                     TAYNKSVGGAVIHPSINAFQLAEIASLNNRVFRTLGSPIVIAHDEWLEIKLENTEDYL
                     VTVDQLNVAKANIRSIYYRIAEERIHFASYRHMHFWHRVKDAFISED"
     gene            complement(26903..27607)
                     /locus_tag="ACG8KR_000668"
     CDS             complement(26903..27607)
                     /locus_tag="ACG8KR_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HFQ0200067.1"
                     /translation="MERDWEAFLAPYEQTVSELKVKLRGIRKQFREQNRHVPIEFVTG
                     RVKPVDSIVTKSQLRHIPMGRLEEEMSDIAGLRIMCQFVEDIHQVVEIIRKRKDMKVL
                     QERDYITNKKASGYRSYHLVIEYPVQLISGEKKILAEIQIRTLAMNFWATIEHSLNYK
                     YQGVFPEEMSERLQRAAEAAYQLDEEMSSIREEIQEAQRLFSHGRGKSDDVYYRTLLK
                     KESEREEAGDESSDRS"
     gene            27738..28313
                     /locus_tag="ACG8KR_000669"
     CDS             27738..28313
                     /locus_tag="ACG8KR_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HFQ0200068.1"
                     /translation="MTQMLEIEYKSMLTKQEYEIFIAHYQLTDKDFRVQTNIYFDTVD
                     EQLKKQNCGLRIRFVGHQAEYTLKTPAEKGRLETTDTFITEEAEAFIFEKRLPRSGAV
                     FQKLSDLNIDPASLIQTGKLTTKRAEFPIEEGLLAIDESWGDNLHDFELELEVGDASV
                     GKIAFINFLKRFSLPYRPAKNKIQRMLEAKN"
     gene            28431..29108
                     /locus_tag="ACG8KR_000670"
     CDS             28431..29108
                     /locus_tag="ACG8KR_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP adapter SpxH family protein"
                     /protein_id="HFQ0200069.1"
                     /translation="MIEIYLFVNPLGEYCFEMEQQLLQFIEEEYGTPSKEKMQFRFLP
                     LVNLQTIGDVMQQKGISQNNLEERNHLFSMTYSAALDCKAAQFQGKKKGRRFLIKLQE
                     AVGCNDIPYSQELAESIFEEIGGDLNMFKEDRQSDFVKEVFFSDQKIAREMGITKHPS
                     AVVYNYACDRDFGVLVEVKGTSSLWRITELCKTTDNSYQVFNGEHLHTNDSQFHSRTS
                     HLHLLSN"
BASE COUNT         8567 a         6169 c         5232 g         9143 t
ORIGIN      
        1 aatacagtat tatttcggtt gcttgctgct tcacctagcc cttgtactcg tgtttgattg
       61 gcgacttgac caaattgatt cccgacgtct aataaaataa tagctaaaag caaaatggcc
      121 aaatggcttc cgccaaccaa taaagcaata aagcttattg tttgcataac aagtcccata
      181 tacacgattt ttctttcaga ataagaatct gccagtcgtc ccacgatagg tgcagcaact
      241 gctccagaca aaccaaaaat cgctaatatc cctgcttcga atgttcccca attgtaggcg
      301 ggactgctga tataaaaaat caacgtagac caaaagatcg aaaatgttcc aaacatgaaa
      361 aatccactga ctgccgcttc tcttaataga cgttgagaag caactaaatg cggcaaactt
      421 ttaagtgaat caaggtatga aacagctgta ctactttgct ggacagaacg cggcattttc
      481 cagtaaagca gaagtgtaag aattccgact gcacataaag caaataaata gatcatccgc
      541 caagaaaaaa gactggcaac aataccagat accgtccgag aaagcaaaat ccccacgagt
      601 aaaccgctaa gcaatgtacc catgaccttc cctcttgctt ttggtcccgc tagtaccgca
      661 ccgtaaggaa tgatgatttg cggcacgatc gaaagtaatc ctattaaaaa agaagcgcat
      721 gcaaataacg taaacgaagg cgcaaaaaaa gcggccaaca gagagacggc agataacgct
      781 accattcgaa tgatcaattt tggacgatcc accacgtccc ctaaaggaac taaaaacagt
      841 aaccctaacg catagcctaa ttgggtcaac atcgtcaata tcccgactgc acttgtgcta
      901 acagataaac tagctgctac ttttgttcca atcggctgga tgtaatacat attggcaaca
      961 acaacgccac aagtgattga taatagtaaa ataagcgaag ttgttaaagg tgttttttca
     1021 ttactcttca ttgatttccc ccatttccta tcttagttta caacttatgg aaaagattgg
     1081 aaatagaaaa atacaactat tcgccacact tattatttgt gcttttgttt tccttgtttt
     1141 ttcttgcgat ctttataaat gaagacaact aaccctacta gcacagtaac acttgcaacc
     1201 aacgtgactg cagttttgtt atatccgtga aacagagctt ctccaccaca cgcatacaac
     1261 acagacgaag ggatgactcc aatcacgatc gacaatatga tttgctgtag aggcaatttc
     1321 atcgcatctg cagcaaaatt gaccatagaa gatggaataa ccggtatcat atagcctaac
     1381 attacaccta tttttggatg tttcatttgt cggattgctt gcacccaatg attcgtttcg
     1441 gttttcttat ctaaaaattt taagcgatgc ataaggaaaa ttgcagtgat gttaccaaga
     1501 gcattaccca aaatgttgat gatcgcacct gtcagtggtc caaagctcaa accagcaatg
     1561 actccaacta ctgaagtagg aatacctgga acagcacaca tcaccgctgt caatacgatc
     1621 aagagtatcg atgcaaaagt cccatgcgaa cgaatatgtg cgagtagctc tgccttactc
     1681 gctctcggat ttaaaaataa ttggatgtct gtctgatatt ctaaatacaa ccgatagccc
     1741 actaccagta tcagaatgat tcccaacgag atcaacatca atcggattat tttctttttc
     1801 atatttcctc ctgaaaaaaa tctatattca ttaaataata gtttaacata aaaacaaaaa
     1861 agcaccgaaa tccaacatag gatctcagtg cttttaaata gtaaaaatta tttaacagtt
     1921 acagatgcgc caacttcttc aagtttagct ttcaattctt cagcttcttc tttagatacg
     1981 ccttctttaa ctggtgcagg agcgccatca actacagctt tagcttcttt caagcctaaa
     2041 ccagttgctt cacgaactgc tttgataact ttaacttttt ggtctccagc tgcagttaat
     2101 tctacagtga attctgtttg ttcttcagca gcagcagcgc caccagcagc agctacagct
     2161 acaggagcag cagcagatac gccaaattct tcttcaatag ctttaactag gtcgttaagt
     2221 tctaggatag ttgcttcttt taactcagca acgatgtttt caatgttcaa tgccattata
     2281 atttcctccg tttaattgtt cttttttatt taatgcttta tgcggcaatt aagctgcatc
     2341 tccatcttct ttgtctgcca ctgctttgac agcgtaagcc acgttgcgga ctggcgcttg
     2401 tagtacagat agcaacatag atagaagtcc ttcgcgattt ggcaattttg ccaatgctgt
     2461 gatttcttcc aatgaagaga ctttaccttc gatgataccg cctttgattt ctaatgcttt
     2521 tgcttcttta gcaaattcgt ccatgatttt cgctggtgcg actacatctt cgttactgaa
     2581 tgcaacagct gtaggtccag tgaatacttc gtccatgcct tcaaggccag cttttttagc
     2641 tgcacgtgaa agaatagaat ttttgataac tttcatttct acgttagcat cacgaagttg
     2701 tttacgtaaa cgtgttactt catcaactgt taatccgcgg tagtcaacga ctactactga
     2761 tgcagctgct gtaaattttt cgcttacttc atcaacgatt gctgcttttt ttgcgataat
     2821 tgcttcactc atttttgagt ttcacctcct gaattttgat ggaagagttt tttgcactga
     2881 aaaggcaagt cacttttata gttactttgc tcaaccagta ttattcatca cctattcact
     2941 tcgttcatag tgattctact cgctttgctc gaaaggaaac tcctttcgct ttgctcaacc
     3001 agtattgttc atcatctctt cacttcgttc atagtgattc acaataaaaa actctatgcc
     3061 accgcagaca tagagggact attgaatata ttcaataaac gtcctcggta ggaaattaag
     3121 gcttgatgcc acctactgtc ttcggtacaa gttaattatt aacctcaact agcttagcat
     3181 gctaaactag ttgagtcaag aattattttt cagttggatc aatcaacatt ctaatattta
     3241 attagaaaga agcttggtca acatggatac caggtccaaa tgttgttgtg atcgttacat
     3301 ttttcatgta ttgaccttta gctgctgatg gtttagcttt tagcaataca tcatggattg
     3361 ctgcaaagtt ttcaactaat ttttcatcgt caaatgaaac ttttccgatt ggtacatgga
     3421 tatttccagc tttgtccaca cggtaagtta ctttaccagc tttcacttcg tttactgctt
     3481 ttgttacgtc cattgtaact gtaccagttt tagggtttgg cattaagcct tttggtccta
     3541 atactcgtcc tagacgacct acagtagcca tcatatcagg tgttgcaaca actacatcaa
     3601 agtcaaacca tccgttttgg attttttgaa ccatgtcgtc gtcacctacg aaatctgcac
     3661 cagctgcttc agcttctttt gctttgtctc ctttagcaaa aactaaaaca gtttgtgttt
     3721 taccagttcc gtttggtaat acaacagcac cacggatttg ttggtctgct tttttagggt
     3781 ctacgttcaa acggtatgca acttcaactg ttgcatcaaa ttttgcaatg tttgtttctt
     3841 ttgctaaagc tactgcttcg gcaacaggat aaactttact tgaatcaact ttttttaatg
     3901 catcttgcat ttttttgctc tttttagcca ttttggttcc tccttgattg tggttataac
     3961 ggttgtacct cccacgtgtt ctatatcttt caatatagag ccaactgaga agctgctgtc
     4021 ttagggtgta tagattattc tacagtgatc cccatgcttc gtgcagttcc ttcaaccatg
     4081 cgcattgctg cttcaacgtt agctgcgttt aggtcttcca tttttaattc agcgatttct
     4141 cttacttgat cgctagaaac tttcgcaact ttatttttgt ttggttcgcc tgaacctttt
     4201 tcgattttag ctgctttttt caatagaaca gcagctggcg gtgtttttgt aacgaatgtg
     4261 aatgaacggt cttcgtatac agaaataaca accggaatga tcaaacctgc ttgatcagct
     4321 gtacgagcgt tgaattcttt tgtgaatccc atgatattga tacccgcttg acctagtgca
     4381 ggacctactg gtggagctgg agttgcttta cctgcaggaa tttgcaattt aacgattttt
     4441 tctactttct ttgccacgag acatacctcc ttaagtccgt gatgtggtta attggagatg
     4501 catatttctc ctcccacgtt ttatctgatc tgaaaaaatc cagacaaata atgtgcaaaa
     4561 caatgcacac tggtacagta tagcaaattt atttgttttt tcaagatttt tttctgctat
     4621 ttttacattt tgcttgaata aacgctaaat ttattctaaa aaaacaacaa tttttcgttc
     4681 tcatttcttc ttttatgtta cacttgcgct agcatggttc aacagatgaa agcaaacaat
     4741 aacacggttt tttagaccct tgtacaaaga ttcctacata acaaaatgag gtggagaaaa
     4801 tgaaagattt aaggtataga agtgtctttg atattattgg acctgtgatg attggtccaa
     4861 gtagctctca cactgccgga gctgctagaa tcgggaaaat catacgtagt atatttggcg
     4921 aacagcctga ttctgtagat atttatctat atgaatcatt tgcaaaaacc tatcgggggc
     4981 acggaacaga tattgcatta gttggcggcc tgttagggat ggaacccgat gatgaacgtt
     5041 tagctgattc tttgaaattg gcatatgaag ccggtatgga agtcttgttt gttcctaaga
     5101 gtgaaaaagc cgaacatcca aactctgtaa agatgcttct aaaaaaagga acacgaaagc
     5161 tttcagtcac tggcatttcg atcggcggtg gcaatatcca gatttctgaa ttgaatggat
     5221 tcaaaatttc tttaagcatg ggtactccta ccttgatcgt tgtccaccaa gatgttccag
     5281 gtatgattgc taaagtgact agtatacttt ctgagaccaa tacgaatatc ggcactatga
     5341 cagtgacacg ggaatcaaaa ggtgaaaatg ccattatgat aattgaagta gatgatcctc
     5401 aagtagaaga aacggtaaaa agactcaaac agctgccaaa tatcgacagc gtaaattatt
     5461 tcgattaagg agctaaaaaa atgtttttat cgattgaaga attagtagat caagcaaaag
     5521 cttatccaag tgtagcagaa ttgatgattg cagttgaaat ggaaatgagt ggccgttccc
     5581 gtagtcacat catcgagatc atggaaaaaa acctctcagt aatggaacaa tcaatcaacg
     5641 agggaaaaga aggtgttacc tccgtcacag gaattaccgg tggtgatgca aaaaaattag
     5701 atgattattt aaatgaggca gactttttaa gtggagaaac aatcttaaaa gctgtacgca
     5761 atgctgtagc tgttaatgaa gtcaatgcaa aaatggggtt gatttgtgct acaccgactg
     5821 ccggaagcgc gggtgttgtt cctggtgtgc tgatggctgt ccgagatcgg cttgatttga
     5881 cacatgaaca gcaattggaa ttcttattta ccgctggcgc tttcgggtta gtcatcgcaa
     5941 acaacgcttc tatcagtggt gcagaagggg gatgtcaagc agaagttggt tctgcaagtg
     6001 caatggcagc agcagctttg gtttgtgcaa aaaatgggac tcctcatcaa gcagcacaag
     6061 cagtagccat tacattgaaa aatatgatgg gactgatttg cgatcccgtt gcaggcttag
     6121 ttgaagtccc gtgtgtcaaa cgtaatgctt taggctcttc tcaagctttt atctctgctg
     6181 atatggcatt agcgggtatc gaaagtgtca ttccgccgga tgaagtcgtt gctgcaatgt
     6241 accaagtcgg acgtcaaatg ccgtcgattt tcaaagaaac agcagaaggt ggcttagctg
     6301 ttacgccaac tgctaaacga cttgcggaag agattttaaa taagagggcg tgataaagct
     6361 ccttaatttc gagtattaag agattagtta tcttcataac aaaaaacgaa gagaggacta
     6421 attgcccttc tcttcgtttt ttattaatcc atcaatttcg tattttttac ttcatcgatc
     6481 gtttttgttc ctgcaagttg catcgtgatc gacaattctt tgttcaggtg ttcaaaaaca
     6541 gatgttactc cttctgcccc acctagattc aacccataaa tcactggtcg gccaatcgca
     6601 actaagtcag caccacttgc cagagctttg aagacatgct ctcctcgacg gacaccagag
     6661 tcgaaaatca ctggtacacg tttgttgact acttcggcaa tttttggcaa tacttcaaag
     6721 gacgcaggac ctccatctaa ttgacgtcca ccatgattgg atacccagat accatctgcc
     6781 cctgcagaga tagcgattac tgcatcttct ggcgattgta ttccttttac gataactgga
     6841 agatgggtga tttctttgat tgttttgatg tcatctggtg tcaagccttg ttttgcagct
     6901 gcataaattt ctgcgattcc ttttccttcg ccattaccac ttgcactttg ttcgctatac
     6961 gcagctaagt taggcattgg taatggaaat tggaattgat tgatgacgtc ttcttcacgg
     7021 taaccaccga gcgttgaatc tgccgttaaa atgatagctt tagcccctgc ttctactgct
     7081 ttgttcaaaa tgaactcatt gaatctgtcg tctttactca tatataattg gaaaaactgc
     7141 ggtgcgcctg gcgctgcatc ggctgcgtct ttgattgtcg tatttgcata agtgctgatg
     7201 gaaaagattg atccagcagc agctactcct ttagctgtat cagcttcccc tttttcatgt
     7261 gccaatcctt gggccgctga tggcgcttgg ataatcggtg tatctaattc aattccgaaa
     7321 acagatgtat gcaaatcggc tgagtcgatt ccgcgtaata tccgtggcat gattttcttt
     7381 gtattaaaag aagtcgtatt ttctttcatc gtccactcat cttcagaacc accgcgaata
     7441 tagccaaaag ctcctgcttc catgcgctct tttacacgac cttctagtta ggcaatgttt
     7501 acaatatcga ttggatgttc ttcattactt gtatgataag tcatagtaga tccttctttc
     7561 atcttcttga ttacatataa atcatactaa acttacaaaa agtaagcaaa catttactct
     7621 cagatacttt tatcgctatt ctttccaaat aaatcgactt acaagctgat ctacttcttg
     7681 gttttcatga agcatactat gagaggcatt ttcacctgtc actacttttt cctctatttc
     7741 atttccgcgt tgcttcaaca acgcatagac cgctaatgca ctgtttaacg gtactgttcc
     7801 atcactcaag tctgtttcat ctagttgacc agcaatcaag aaaaatcgag tggttatagg
     7861 aatattaccg atcatttgct ggtaatcttg atagcggtta gaaacgacag caggcccctt
     7921 tgctaactca tctgtcagag actgcgcact atcatcgaca aaatcattaa aaggcgcgcc
     7981 aattgctgtt aatgtctgaa cttgtggcaa tgacgtatcc tgtccatacg ttcctaaata
     8041 gcgtagtgca ctaactcctc ccatggaatg ccccactatg ttgactttat ctatgccgta
     8101 gttttctttc agatacaaaa gacaagtttt gatccattct gcttgattcc actcgttatt
     8161 tttattatct tcaaacaata cttggaccat cggattgtct gcttttccag acaaatctcc
     8221 tgttgcttgt atttgtccat ctgttcctac tgttaaaacc aattctttta ctgcttgctg
     8281 atttttttcc aaacgccgca acatcccttt aaaggaattt tctgtcccgc tgtagccgtg
     8341 gaaaaaaagt gttggaatag tggttttttc tatcacttta ctggattctg ccgttttttc
     8401 tttatcagaa acttcagtag tatttatctt cgtcgtctgg caaccactta acgcaaacaa
     8461 actgataagg actaaagctg ctgcagttgc acgtttgcat aaatttttga ttggttctgt
     8521 catacacctc ttcctttcca tatagaaaaa agaaagactt gaaaggccaa tcatatgatc
     8581 gacatttcaa gtctctttat taatcgattt tatctacttg atcgaaatca agttctgtac
     8641 tggtttcacg tccaaacata tcgatgttga ctttcaattt ttctttttct ccgtcaactt
     8701 ctgttacttg tccttctaat ccagcaaatg cgccttcgat gattttcacg acttcgccaa
     8761 cttctacttc tagatcattt tgtcgcaggc tcataccgat agaacgcaag atatgattga
     8821 cttcttctgg aagtaatggt gctggtttgc ttccggctcc gtgtgaacca acgaatcctg
     8881 tcacaccagg tgtgttgcgg acaacatacc atgaagcgtc tgtcatgatc atttcaacaa
     8941 gtacgtatcc tgggaatgtt ttatgaacga tttctttttc tttcccattt ttgacttcag
     9001 tttctttttc ttcaggaaca acgacacgga aaataaagtc ggccattttc atactttgcg
     9061 cacgagattc gatatttgct tttactttgt tttcatatcc tgaatatgta tgcaagacat
     9121 accagttttt ttcaaaagat tccattgaag gctcctcatt tcattgtaaa ataaaaaaac
     9181 ctcagtacac ccgaagtttt tctttcgctt cattatagca ctaaaagtac gttgtggcta
     9241 gcgagttatt ttaaaatcca gccaaataag ctttggatgg ctgtatccat tacgtaaaac
     9301 atcaatccga aaataataga agtttcgatg actactaacg tatctttacg gagttgtttt
     9361 ctgcttggcc aagttacttg tttgatttca tcttttacgc tttttagaaa tttcatgaga
     9421 caacctccct tgctactgtt tatttcgttt ctttatgtaa tgtatattga ttacaatatt
     9481 tacaaaactt attgatttcc aaacgttcgc cgctttttcc ttcactgact gattttgtgt
     9541 agttgcgaga cccacaaacg gaacaagcga gtgctgcttt tttctttgcc atttacttct
     9601 gcccccttgc tgagaatcag acttatactt cataacagta tcacgtaact ttttacttgt
     9661 caatttgtga aataaactat ctattgtttt ggataaaatt atgatatgat taatttgaaa
     9721 ttaagctaaa atgagacaat tggaggtata gcgatgctat taaaaacact tgtatataag
     9781 aaaaaagatc tgacaaccgt tacagagact agtacattag aagaagcatt aaaaatttta
     9841 gaaacttccg gataccgttg tgtgccgatc ttagattctt caggtaatat tttccgtgga
     9901 aacatttaca aaatgcatat ctatcgtcat aaagcaaatg gcggtgatat gacgcttcct
     9961 gtcactcatc ttttgaaaaa cgctacaaag ttcatttacc tggatacttc tttcttcaaa
    10021 gtattcttta cgatcaaaga gcttccttat attgctgtcc ttgacagtga aaatcatttt
    10081 tatggtattt tgacacatag tacgttgctt aatatgctct ctcaatcttg gagcgtagaa
    10141 caaggaagct atgtactgac aattgcttcc acaggaaagc aaggagattt ggctgctatt
    10201 gcaaaaatca tttcaaaaca cagcagcatt gccagctgta ttactcttga tatcgaaaaa
    10261 gacgaataca ttcgacgtac attgatcacg ctaccggcta acactgaagc gaaagtatgt
    10321 gaaacgatcg tcaaccactt agagaaaaag aattataaag tggtagaaat tgaaaatttg
    10381 caaaaccaag aagcataatc aaaaagcgaa aaaagaaaac agctaatctg ctttcttttt
    10441 tcgcttttta taattagaca cgcttttcaa aatcaagcgc gcgatctaaa taatacatca
    10501 atggtgctga aacagccatg acgataaact cacttaatgc aagcgtgcca taggttgtcc
    10561 aaaatgcttg gccacctgtt ggaactaaca tataagcaat cagtcccatg ctgatggtaa
    10621 agaagatcgt gttcaatact aaacgcatct ttacattttt cactttgttt tgcaataaag
    10681 ctgtcaggcc taaagaaagc aatgtttggc cgccaccaaa aaccacatcc atgataccaa
    10741 aaccaaagaa ggcattatag acgatgaccc cgcctaaaac tccccaaagc aatttacggt
    10801 taaagacaac taaatgattc aaactttctg aaatacgaaa ctggatcggc cctgaggata
    10861 caggcgcaac taaaatagtt aacgctagat aaagagccat gatgatgcca tttacaacga
    10921 ttacggataa ccgttgtctg cttttcgttt tttgcatttt ctctcctctt ttctcctagt
    10981 ttttttaacg cgggatggtt gatgaaccgc ggttatgtga aacaaacaat aattataaca
    11041 gacttttcat ttatttcaat ccgtcttatc tgcttttaaa aaagcaaaag cttaaacaat
    11101 gtaaacgttt gctaaaaatc gtgggatatg gtataaagta agtgtaaggg gtcataagaa
    11161 ttcccagtaa agcagacgaa gtaggatctg caagaacaga attgttgtga aacttaaata
    11221 aataaaaaag gatgtgggaa tttgagcaac ctcttactag agaacggtct agcaagcagc
    11281 tgaagatcta agtggacatg ccgaaccttg gaacttctcc ttacgtaagt aatggatcat
    11341 gcgcttgcta ggcttttttg tgtttttcgg tcttcggacc tttttcattt tcctagactt
    11401 ctgctataat aaccaagaat taattttgta acagattttt ttgaaaaaac aagtttcttt
    11461 cattcaaaaa atagaaaaag cttgctaatt attggttaac cagcaaacag gagtattaaa
    11521 atatcttgtt ttattgaata gcaggaggaa atcatgaaat tccattatca tatattgatc
    11581 aatccatcgg caggcagcgg aaatggctat aaagtagcag aaagaatttt accggtatta
    11641 aaaaataagc atattgacta tacacttcat tatagtgaat acaaaggaca cgatgctgag
    11701 attgctgaaa ctttggcaaa agaaacattg attccttgga tagaagaaga acagaaaaca
    11761 atcgatactt tccccctttt gatcatcgta ggaggtgacg gcaccttaca tcaggtactt
    11821 gatactttct atcagatgga agtagaattc ccagttgctt atattccagc aggttctgga
    11881 aatgattttg ctcgaggggc aggccttcca aaaaatccca aaaaaggact tcagctgatt
    11941 ttagcagctc aatcaccaga aaaagtccac ataatggctt atgaagaaaa aataagtgaa
    12001 aaaaaaggaa tagctgttaa taatttcggt ataggattag atgcagctat cgtccatgcg
    12061 acaaatcatt cttccacaaa aaagcgattg aacaaataca acttaggatc attttcgtat
    12121 ttattctcta ttctccgtgc tttattcact caaaaaggat ttccaattct ggtagatgca
    12181 ggaggaaaac aattaagttt ttcaaacgca tttttatgta cagcaacgaa gcatcctttt
    12241 tttggcggag gaatcgcgat tgttccaact gctgatgcgt ctaaaccagt cattgatttt
    12301 gtcgtggtcg aacgaatcaa tttctttaaa atcttgtggc tcattctttt acttatacag
    12361 aaaaaacaat tgaaatcgaa atatttccat cactacacaa caaacagatt acgtatcgtt
    12421 tcaacaacac cgcaacatgg tcaagaagat ggagaagaaa tggaaaaacg accttttgat
    12481 atcacaataa ccacaactaa acagttgttt tggtgttaat aaaagaggtt ttgtcagaag
    12541 ctacatctgg aacaccgttt attcggaaaa aaggactgtg acacgacttt tgtcacagtc
    12601 ctttttttac tatgcagtta attctttgcc caattgggtt gccatcgata gtttctttct
    12661 ttacccaaaa taaattggat cattgaaaac accgcagcaa aagcaactaa gacacaacca
    12721 ttgaaccaaa aagattctgc aaatgtttga ttgtaactga gtgtttcact gatcaagtta
    12781 ttattttgaa caaaccaata tgttggtgtg aatgccgcta ttttgctaac catttctggc
    12841 aagtattctg ctggtacaaa caccccaccg ataaagcaac tgcctgtgat aaaaatatct
    12901 ttgatcccac cgctggtttc tgagtttttt accaatgaag tgatacaagc cgaaaagcta
    12961 atgatcggca ataaaaacaa gaaagtattt aaaatatgat accataccgt tatatcccac
    13021 gaggtgtgga tcactaacag aaccattaga agtaagaagc cactgaacaa cagcagcgag
    13081 taactaatca aactaaaggt tactttgcga ttcaagtttc gtttggtaat gggcgcactc
    13141 tggtttcgtt tactgatctc tttgcgatta aacgctagat tgatgacgct gaaaccgcta
    13201 aaaatggata gaaacaaccc ataagacaag aaattaaagg ttgtgcccgt caacagcttt
    13261 ttcatccgct gactatatcc cgcacttagc tcaacttctc cagtaaccga gagtgccatt
    13321 tgtgtctgtt ccatagctgc atcgattgtt ttatcttctt cttgaaacag aatcagtgtt
    13381 tgcaaaaagg tgttcacttg ctgtgtaaca actgttttag aaaaggcatc tgggcgactc
    13441 tgtatcttga tcgtcgccat ctttcctact tcgacttgtt cggtaaagtc tgcaggcaga
    13501 tgtaagataa tgtttacacg attaaaataa agagcatcat cgatttcttt ttgtgaggtt
    13561 ccatccaatg taacgactgt ttccttgtca ttcaaatatt tacgaaggcc atcgactaca
    13621 ggcgactctt cttcactaat caaggcgatt ttcgcatctt gcagagaggt attttcaaca
    13681 tcaatatttt gcgcaaaaaa gaatgtggca acaacagtga taattacacc tatcagtaaa
    13741 ttcgatttat ttgctttaag caatgtccaa atcgttttat aaagcgtcat aacgcacccc
    13801 tctttccatc caagcatttc ctaacgcaaa aaggaaaata aatccgatca accatagaag
    13861 attggtataa aaactagcca aatcagtgta gaaataaagt ttatacaaac tttcgctgat
    13921 caagttgacg agatttaatt gaccaacaat cggaatatga agatcaatcc aatacttcac
    13981 ttgttccgac cccatcatac cagcaaaaaa gctcatcgca atcgtcactg aaatcaagat
    14041 gctatccttt tgcaccagat tcatttttgg tatcaaatta ccaaccaatg tacctaacaa
    14101 aatcgcacac aacgacccca gagcggtaac aatcatcaac cattgccaac gttcaccaaa
    14161 gtctacttga tagataaaac gaaagacagc taacataata taaatctctg tcaaaacaat
    14221 cgtaaagctt gctgccaaat tagcgattga aactaacgat tgctttctag gaatcaagga
    14281 caagcgaata ccatttgacg attgatttgc ttgctgatcc tgtacattgc ggacccccca
    14341 catcgtcccg tagagaatcg ccattccaat cagagtgaag aaataaaaac ttttgaaact
    14401 catattacga cctgcatgaa cttcttgaaa agatacttct tgttcaaaaa cagtcatgat
    14461 gggcgcgaga tcaacgttct ccacttctgc cactttactc gaaattatac caatacgctg
    14521 taaaaattga tccatgacgt ttttcatcaa cgtctgactc attcctgctt gacccacatg
    14581 gagcgaaaca gatgaaccat caatgagata aaaaccagcc aaatcatctg catttagctt
    14641 ttcattcgct tctacttccg atagttcctg tacttcaaat aatggtttat cattgacttc
    14701 gacttgttct aagactgtgg taaaattttc cgaaagcgct gtttgatccg ctttgattac
    14761 aattccagta ttaatcgttt caagattgcc accttgatca atctcgccaa aggccgccat
    14821 aaacatcaat cctaaaagaa ttggaaaggc caatgtccaa aacaacatgg atttatttcg
    14881 cagcagtatt tttgtgcgat atttataaaa atgccaaaac attatttccc ctcctctagt
    14941 ctctcaaatc cttgccagta atctctaaaa agacatcatt caaggacgct tctttggttg
    15001 ctatttttcc gtaactgatc ttttcttctt gtaagattgt aagaatcggc aacaatactg
    15061 tatttagttc agtagttgtt acttggagct gacctttttt ataagcgatt ccatcagaag
    15121 ctattgttcg ctgaatcgct gacaacacat tttctgggag taatgggatg tcgatcgtca
    15181 aacgatccgc cgtccgtacc atatctttta gagattcttt ggttccttta gcaatcacct
    15241 gtccttgatc catgatcacg atctggtcgc aaagaatttc tacttcttcc atgtaatgtg
    15301 ttgtatagac aatggttgct ccttgtcgat tcagctcctt gatactttct aaaattttat
    15361 tccgactttg ggggtctaca gctactgtcg gctcatctaa aaaaattaac cgcggcttgt
    15421 gggcaatccc acatgcgatg ttcaaccgac gtttcaagcc accactaagt tctcctggac
    15481 gaaacgaaac atatttttct aatcccacta attgaatcac ctcttcaatc aaggctttcc
    15541 ttttctcttt ttcggaaaca tataaaccgc aaaaataatc gatattttct tgcacactta
    15601 gttcgtcaaa gaccgcaacg tcttgaggaa cgaccccaat cattgccttg acagcataat
    15661 tttcaggagt cattgttgtc ccgaacaatt taatggttcc tttgtcataa tttaacaaag
    15721 aaagcataca gttgatggca gtcgatttcc cactgccgtt tggacctaac agacctaaga
    15781 cttctccttc ttgtacttgc agatcaaaat gattcaatgc tacaaaatct ccatagcgtt
    15841 tgactaattt atcaatttcg ataatcatca tgaaaaactc cttccaaatt tcttgttgat
    15901 ctgactataa taaagtgcaa gaaaagaaac tagtgacgat tggctaaacc acacatgaca
    15961 tttgtcacaa tgaaggagtc tgctatgcat aaatctcggc tactcaacga aactagcttt
    16021 ctactggtta tctcattgct tttgctggta agtcaaggat tcgttgctat gcaagttatc
    16081 tatttattga tcgccgtttt attcactggt gtcagttatt tactgcctac aaagccaaat
    16141 tggattttcc aagttattct atttctttta tgtgcctttt tacctgatct tgtcctgttt
    16201 ctgccagcga cgatgcgagt gatcgcccct gaagaaagcc accttgatta cttgtttttg
    16261 ttaggttttt tgttgatttc gacggttggt aatcatcccc ccatcaaggt cgcctttgtc
    16321 gtaattttgc tgtttttttc cttttactta cgaaaaaaag atcaacgaac gaccgatctt
    16381 gagaaaaagt acattgtctc caaagatact cattgggaaa aacaacaaga gttatcggaa
    16441 caaaataaag cattggccct gtcacaagaa acacaattag ccttgcaaat tgtggaagaa
    16501 cggaaccgaa ttgcccgaga catccatgac aatgtcggtc atctgctgtc aagtgcgatt
    16561 ttacaaattg gtgcgcttga aatgatcaat caagaaccaa aactaaaaga accattagaa
    16621 aatttaagtg ccaccgttca cactgggatg gatcggattc gtcaaagtgt ccatgacctc
    16681 catgagacat cgatctcttt tcaacactcc ttagagtttc tgatcgctga ttttccttct
    16741 tctgttacga ttgaaggaaa cttgtttgaa ggattgaatg aaacgcagca aaactgcttc
    16801 ttaatggtca tcaaagaagc cttaaccaac agtatcaaac atagtcatgc caaaaccgtt
    16861 gttttacatt ttcgaacgtt gccggccttt tatcgcttgc aaatcacgaa tgatggcacc
    16921 agcccacaaa aaaattctgc tcccggtatt ggtttgaaaa cgatgcgtca acgtgttcaa
    16981 gaacaaaagg gacaagtcca tattttccaa gaaaaccagc aatttcagtt aacgatcatt
    17041 ttaccaaggg agggacacca atgataacag tagtcattat tgatgatgat ccatttgtga
    17101 cgaaatcgtt acaaaccatt ttagaaagca cgaaagagat ccgtgtgtta ggcattggtc
    17161 attgtgccaa ggatgcgcta gtcttatacg aaacccatcg tcccgatgtt ttgctgacgg
    17221 acatccgcat gcctaaacaa acgggcatcg atgctgccaa agaaattttg gctaaatttc
    17281 ccaaagccat ccttttgctt ttgacgacat tcaaagatga agaatacatc cgcgaagcat
    17341 tttccatcgg tgtcaaaggg tatttaatca aacagaactt gcaagcgatt attccttctg
    17401 tcaaatccgc ttataatgga caggccgtat ttggtaatga aatcatcgag actttcactc
    17461 aaatgatgaa gaataaaccg acgattgata gtcatgctta tgcttctttt agtgaacgtg
    17521 agcttgcaat catcaaagaa gtagctgccg ggaaaaacaa taaagaaatt gcagatgcct
    17581 tgtacttgag tgacggcact gttcgcaatt acatcagcca attactagaa aagctagatt
    17641 tacgggatcg cacgcaattg gctatctatt tttatcagca tttgcaataa gtcttatcta
    17701 acacaaaaaa acctgaatga tgctccttcg catcattcag gtttttttca catcttctat
    17761 tcaattgtcc cgcaaaccat ttcctcacga ttgaaagaat caaggattcg cagggctgcc
    17821 aggattcgtt ggattgttcg gcgtgttagg cggaaaaata ggcgccttgt ctaacgtttt
    17881 ttctagtttt gattcttcac gatcgtctcg ttctacatca ggactttcat tcaatttgaa
    17941 ttctttttcc atctcatcta actttttaaa gtcattgcca gattcttttg ctaatttcgc
    18001 taatttatct tcggtttctt tagccataaa gtaacgcccc ccttttgatt tcagcttagc
    18061 atatcacact gaaaaaacaa aagaaaacgc taaccaacga tcatttttag ctatttctca
    18121 tctttaaaat caagcatttt ttgccggtat aaaatcgtag ggagaactgt tttccatacc
    18181 aatcatcgga tctactaact ggttatcgat cacttctgct gtaagaacga aatctttaga
    18241 aacagcaggt tctacctctt ctttcttttc ttctccaaca aatgaagaaa acagatctgg
    18301 ttccactgtt tccattactt ggtttttcgt ttcattcgtc ccactgtcat cttctacagg
    18361 tagcttttct tttacaaccc gtggctcttt tgtatagttc ccatcttcaa atggatcatt
    18421 tccgaccaaa gaatcttcat aagctgctag tttggtccgg agagtgatat tcattttatc
    18481 attatccatg atccttgatt tcaacgtagt catccggctt gctgattcat tcgctacaca
    18541 agtgacatag gcttgctctt cccctaacaa aatcaacagt tccttactgc gagtcactgc
    18601 cgtgtagagc aaattacgct gaagcatccg ctgatattga tggaccatgg gcaaaataac
    18661 catcttgaat tcactgcctt gtgatttatg gattgagcaa caataagaaa gagtgatttt
    18721 attccattcg tttcttttat aagtgacctc atttgcatca aactggataa ctaattcatc
    18781 cactttatct tcagaatctt ttgccaagat gatacccgtg atttctccca tatctccatt
    18841 gaatacattc aattcgggag tattgactaa ttggaggact ttgtctccta tccgataaac
    18901 ggtgtcgttc caatgtactt ccttttttcc aggtttccct ggattgaata tctcctgcat
    18961 cattttattt aacgcatcga tccctgcagg tccccgatac attggtgcta atacttggat
    19021 atcttgtgca cggaatccct tatttttcgc tttttccaca attttagaga tcaagggttc
    19081 aatttgataa gcatcacttc ggaaatagga acgatctttt tgattctttg tgaaatcttt
    19141 tggcagacgt ccttctttga tctcatgggc aagaggaata atactagaac catcaccttg
    19201 ccgataaatc tgattgagtt ccattttggg aatttgttcg atttccaaaa gatcatggag
    19261 tacttgacca ggaccgacag agggaagctg atctttgtct cctactaaga taacttgcat
    19321 attcgtcgga atcgctttga ataatgtgtt agccagccat gtatcgacca tagacatctc
    19381 atcaacaatc aaaagtccgc cttctaattc tttcgtactg actgaaggtg ttttttcccg
    19441 tccattcagc cccagaagac gatggatcgt actggcaggc agacctgtcg tttcgttcat
    19501 tcgtttagct gctcggccag ttggtgctgc aagtagaata ggaaaaattt cttgggtata
    19561 ttttgaagga tccaattcca gtccgttcaa ctctgcaaaa agattgacga ttccgttgat
    19621 cacagtagtt ttccccgtac ctggtcctcc tgttaaaata aatagagggg aacggattgc
    19681 ttcagcgata gcttgttctt gcgaaggtcc atactgtatg ccgaatattt tttcgatacg
    19741 ccgaatattc ttctgcactt ctttttcagg ataatggatt tcttttttac gactgagaag
    19801 gcgctggatc gaattaccga tcccccattc agaaaagtac aaactgtttt cgtaaagatt
    19861 agtctcttct tgttgtatct ttccttcttc aacaagttgg ataattacag tagcaacctg
    19921 atcaggctgg atttcaattg ggcgacttgt ttctaaaata cggatcgttt gctgaagcaa
    19981 tgtctccgcc ggtacgtatg tatctcctgt ttgaactgca tgctgaaaaa tctgatgcaa
    20041 gatagctgct cgaattcttc tcggtgaatc tgctgcaata ccgatttgtt cggctagatt
    20101 atctgcacgt ttgaacccga ttccttctat gtcttcgact agctggtacg gattttcttc
    20161 gatgatatcc aaagactcgt tcttataggc ctggtagata ctgaaagcta actgactacc
    20221 gaacccgtaa cgattcagtc ccatgatcac ttgatccatc ccgtggttta gccgaatcgt
    20281 ctcagcaagc atttctcttt ttggcttcgt caaaccggag atttgtttta ataccgaagg
    20341 atcagcaatg attttttcga tagcatcttc tcccagcaat tctacaattt tttctgctgt
    20401 tttcttcccg attcctggga atttttcgct ggaaagatac gctacgatcc catttgcagt
    20461 tgttggcttt tcttgctcat atctttcgag tttaagctgc cttccgtatt taggatgatc
    20521 cactaattct ccataaaaac gataagtctc attttcttga atatcgccga aacttccggt
    20581 gactacaatc tctttttcac ggtaatcagc tgtggtttca ctgatgtgta ccaaaagcac
    20641 tttgtaaaaa ttactaggat tttgaaaaaa aatcgcggca actgtaccaa cgaaataagg
    20701 catttcttct ggctgcatcg gaagccgcct ccttcttcta tagtaaaggt tctggtgctg
    20761 tttcagttcc taaaaaatct gtttggttcc atactttcag ttcatatggc aataatctgc
    20821 aaggctcttc agtctctaaa atcgtcaaac tactattgaa caatccaccc atttcacgca
    20881 actcacttag ctcttttcct gtcatccatt gaatggctgc agttaaagaa gctccatgtc
    20941 ctacgaataa aagtgggcca tctttatgtc gggcaaccgc atctgctaca acagtttcga
    21001 tacgtgtgat tgccgattca atcgtttctc cgtgaaaagg agaaggatca tacagatcta
    21061 aatgattacg caaattcggc agattatccg gatatttctg gtacatttca tcgatcgact
    21121 gaccttctaa agtacccagc cctagttctc ttagcttatc tgtataaaaa atctctaccg
    21181 gacgagcaag ctctttgttg ataccttctg ctgtatcacg tgctcgcttt gctgtactgg
    21241 aataaattgc tttaaaagga acatatttta cctttttccc aaatgctttg atttcttcgt
    21301 aactttgtgg caaaagcgga gaatcgccat tgctcccttg aaaacgtctt tcctgattcc
    21361 attgtgtctt cccatgtcgg gtaaaatata gttccatacg cagcaaactc ctgtctttct
    21421 tttattttac tctataataa actgtctaat ccagcttgtc attttcatag tgatgcgtca
    21481 attctaaatt agggaatcct tgctggcgaa gtgcttcata cactatcatt gcagctgtat
    21541 tcgataaatt caatgagcgg acatgctcat cattcatagg gatgcgtaaa cacttttctt
    21601 cgttttcacg cataaatgtt tctggcaaac cggttgtttc ttttccaaat aaaaagaagt
    21661 gctcttctcc atcagcaaaa ttttcgtcac tatatacttt atgggcaaat tttgagatca
    21721 aatgtagttt acgattacct aaaaatgtta aaaaagcttc taaattactg tggtaagtaa
    21781 tatttacatc attccaataa tccaaccctg cccgttttag atgtttgtca tcagttgaaa
    21841 aacctagtgg ctcgattaaa tgaagcggag tattcgtggc tgcacacgta cgggcaatat
    21901 ttccagtgtt tgcagggatt aatggttcaa ataaaacgat atgattcatc aggatcattc
    21961 ctctcatatt tatgttcgtt tctattttct gacaatttga tttttgcata aaaaaaaacg
    22021 tatcaactcg gtgtcagtca ctgcgagtcg ttacgttagt gaagctcttc tcactaactt
    22081 ttatcagaac aggaacctga taaaaaccaa tgaaaaaaca acttgttgct aatataacac
    22141 cctcaacttt tttttgcaac ctctactcat taaaaactgc tagaaaaacg ttttctctta
    22201 gttcctttta ataattctat gtcaattgta atcttcttca aaggattctt atataagtct
    22261 gctttgattc gctcttctac ctgccattct aaaggtgaca tggccaaaag atctttttga
    22321 ttttcctctg gtatctcaaa ttcataggtt acacgctgac gttcagttaa tttcattgct
    22381 tcagcaaact tggctactac acgttcattg gaataactct gtttttcagg ttgattcgga
    22441 taaaaagcct gacgtagttc ctttaaataa ttttcttgtg gcaccacctt caacacttct
    22501 ccatcttctg agagtacacg ttggaattct tggtaatgag aaggcgaaaa gatatttaaa
    22561 accgcgttga atgtttgatc agcaaacggc aaattggtca aatcagctac acaccaaaat
    22621 gcttcggcag attgttctgt agctgcgtag actccttctt ttgaaatatc aaatccaaca
    22681 gcattttgaa tctttttcaa ttttgaaagt tcattcaaga agctaccttc tccgcagcca
    22741 acatctaata gcgagacttc ttcaggtaaa tgctgcgcaa tagtctcaat aacgggctga
    22801 tacatgccag attggatcat tctacgcctt ggaacgaaca ttccttgatc gtattctgtt
    22861 ttaaccgcat gggataaaaa ataaagtgtt ccttttttgg aaaggtcaaa acggtgatta
    22921 tttttgcata tcaagccatt atcagacttt tttacttctt cttggcaaac cggacagcgg
    22981 aacagctgat gattttctgc caaaaactgg cgtcctcggt caattttttt taacatagtt
    23041 ccccctcatt tctgttttat cttaccgtta gatgctcact ttggcaactg ctaaaaatat
    23101 gatacaatgc ctagaagaaa gtgaaagata aaggagaaga ttagatgatc aaagaatttt
    23161 gcgctgaaaa ctacacatct attcctctag caattgctag aggagccgat cggatcgaat
    23221 tgtgtgacaa cttagcggtc ggtggcacaa ccccgagtac tggtgtgatc gaagaaacgt
    23281 taagttatgc tggggaaaaa gatatccccg taatgacgat tattcgtcct agagggggcg
    23341 acttcgtcta taacgatatc gaattgaaaa tcatgcatac tgatttgatt gaagcaaaga
    23401 aattagggac ggatggcgta gtatttggtt gtcttactcc ttcaggctgg ctagacgaag
    23461 aggccttaga actattgatc gaaacagcag aagggttgca gatcaccttc catatggctt
    23521 ttgacagtat tcctgaagat cggcaattcg aagcaatcga ttggctggta gaacatggtg
    23581 ttgaccgtat cttgactcat ggcggtcctg ctgacaaaac tattgtagaa aatttcccac
    23641 ggcttaaaga attgattgcc tatacagatg gccgcttaat cattttgcct ggtggtggtg
    23701 tcactgcaga gaatgcagca catgtagcta aagaattgaa cgtaagcgaa gtacacggaa
    23761 ccaaaatcgt ggatcttcag ttataatctt ataaacaaaa aaacatttgc aacaggcata
    23821 atgcttattg caaatgtttt tgttttttta cataaaatag cccataaata aagaagcaat
    23881 attgaaataa atcagtacac tcgtcaaatc gctcaaagtc gtgataaagg gaccgcttgc
    23941 tactgctgga tcaaatccta gacgatccat tagcatcggg attaaactac ctgcaagatt
    24001 cgccactgtg attgcaaata gcatagccat ccctatgaca aaccccaaga taaaattatg
    24061 tttccaaaaa cctacgatta gaaaaatcgt gatccccgta acagctcctg tgacaacgcc
    24121 ggtcaagact tcagccaaaa caagtcgaac aaatccatga tctttctcat cagaaatcgc
    24181 aagtcgacga acagccacag ctagcgactg ggttccagca ttaccggcag ttcctgtaat
    24241 cagcgaaatg aagactgcta aaatactcgc ttcacttacc aagtcttcat agtgactgat
    24301 taaagaagcg gtcgacatac ctaaaaacaa cagagtgatc agccaaggca aacgtcgaga
    24361 cgctgccttt actggatttt cattaatttc ttccacattg accccggcaa gtcctgaata
    24421 atcgctagca gcttcatcat caataacgtc gatgatatca tcgactgtga cgattcctaa
    24481 caaatgatca tcataatcgg taacaggaag agctaagaag tcataatctc taaatgtttg
    24541 tgccacatct tcttggtcgt cgcctacatg aactgaaagc actcgttcgc tcatcagatc
    24601 agaaatcatt gcgtcgtcat cattcacgat taggtcgcgc agagaaataa cccctactag
    24661 ctgaccttct tgattaacga catagatgta atagattgtt tcagctacat ctgcttcgtt
    24721 cttcagcacg tacatcgcag agcgaacagt ttgattggct acgatcgaaa caaattcagt
    24781 tgtcatgatc gcgccagccg tttcatcctc gtaatggaga agttccttga tttcactggc
    24841 atcgtctgcc gacatcaaac ttaaatattt agcaatttgc tttttatcca gcgtattaag
    24901 caagtcaaca gcattatctg tatacatctc agaaagcata tcagctgcat agctaggacg
    24961 catttcatct aggtattctt tcatatgttc gttgtcttct tcaatcacat cgaacatatc
    25021 cgctaattct ttaggcgaca gataggaata gatcagctgc ctgttctctt ctgtaagaga
    25081 ttggtagaat tgcccctgct catagatatg catctctaaa aaactgtttc tgaatgcctg
    25141 aatattattt tcccttaaat cttgagtcaa ctgggcaaat ctttcctcta attcttgatt
    25201 ttcatccaaa ccaatctcac ctcgccttat tcttcaaacc gtctcacaac tgcctccatg
    25261 tctgcggcta aaggctgctt gaactccagc aactcttctg taaatggatg gtaaaaacgc
    25321 agatcataac aatgaagggc ttgtcttttt attcccagat ccattcgccc gccatacata
    25381 tcatcaccga gcagggcaca gccaattgcg gaaaaatgaa ctcttatttg atgtgtcctt
    25441 cctgtatgca gctggatatc aactaacgcg atttctgtta gtcgcttctc tatccagtac
    25501 tcagtctgtg ccgaacgtcc ttcatcagag accattcgct tcaatagcga agaataatct
    25561 cgggcgatcg gcaaatcgat atgtccatgt gccgataaat ctgaaagcct tccactgact
    25621 aaggcctgat attttttcac aaacttcttt tcacgaagtt gcatatctaa ttttgcgtgc
    25681 gcaaatccat gctttgcaaa aagcatcaag ccagatgtat cccgatctag tcgcgtcacc
    25741 acatggatta cttggttttc gtaaccttgt tttttataat aagcctttac acgattagcc
    25801 attgtcccat ttggatggta ttgggcagga atagacgaaa cgcctgctgg tttatttact
    25861 accagaacgt gttcatcttc atacacgata tcgatcggtg tttcatccaa aagtacggtt
    25921 tcatgttctc cttcagcagg aatcacaatg gttaccttat catccttagc tagagaaaac
    25981 aagacatttt ctacttgatc attaactttg atttgaccgc cttgaaattt tattttcgct
    26041 aacagaccct ttgagatccc tttttctttt aaaaaatatt ttacttgctg agaatgatct
    26101 ttctcataat cccatgtaaa ctgcatatca atcctcacta ataaaggcat ctttcacacg
    26161 atgccaaaaa tgcatatgtc gataagaagc aaaatggatc cgttcttctg ctattcgata
    26221 ataaattgag cggatatttg cttttgcgac attcaattga tcaaccgtta caagataatc
    26281 ttctgtattt tccaatttta tttctagcca ttcatcatgg gcaatcacga tcggagaacc
    26341 caaagtacga aacactcgat tattcagtga agcaatctct gccaactgaa aggcattgat
    26401 actaggatgg atcacagcac cgccgacact tttattatat gctgtcgaac cagtaggcgt
    26461 agaaatcgtc aatccatctc ctctaaaact ttcaaacagc tcgtttttga tgtatacatc
    26521 ggctaccatc gtacgattgg cccgtttaat cgttgattca ttcagtgcta aaaaatgctg
    26581 gtccggtgtt tcatccaaat aactgatccg aacatccagc aagggataac taacactttg
    26641 ttcccggttc gttcgtaagc tgtctactag ttcttccagc tcgtaatccc gccaatccgt
    26701 gtaaaaccct aaatgacctg tatgcacgcc caagaaacgc acatcattca gacaatgact
    26761 gtagtgatgg aacgccgaca ataatgtgcc gtctccgccg actgagatga ccaattctgg
    26821 ctcatgatca tctcgttgaa tccctgcttg ttctaaaagt gcgttcaaac gttctgtcac
    26881 ttctatagtc tttgcttctt tattatgaac gatcgctact ttcatcccct gcctcctctc
    26941 tttcgctttc tttcttcaaa agcgttcgat agtagacatc atctgatttt cctcttccat
    27001 gggagaataa ccgctgtgct tcttggattt cttcacggat actggacatc tcttcatcca
    27061 actgataggc agcttctgcc gcacgctgaa gtcgttcact catttcttct ggaaaaacgc
    27121 cttgatattt ataattcagc gaatgctcaa tcgttgccca gaaattcatc gcaagagtcc
    27181 gaatctggat ttccgccaaa attttcttct caccgctgat cagctgtaca ggatactcga
    27241 tcaccagatg ataagaacgg tagccacttg cttttttatt tgtgatgtaa tctcgttctt
    27301 ggagaacttt catgtctttt cttttacgga tgatctcaac aacttgatgg atatcttcta
    27361 caaactgaca catgatcctt aacccggcaa tatcagacat ttcttcttct aaacgtccca
    27421 ttggaatatg ccgtaattgt gattttgtta caatactatc gactggcttg actctcccag
    27481 tgacaaattc aatcgggaca tgtcgatttt gttcacggaa ctgtttgcgg atcccccgca
    27541 gtttcacttt caattctgat acagtttgtt catatggtgc taaaaatgct tcccaatctc
    27601 gttccatagc tgatacctct acttcattaa cattcattgt ttattctacc atatatgcaa
    27661 agttttttcg tactcttgat ttatgcattg accaaaaaaa gaaaattagg caaaatagac
    27721 aaaaaggagt cgatcaaatg acacaaatgc ttgaaatcga atataaatct atgctgacaa
    27781 agcaagagta cgagatattc atcgctcact atcaattaac agataaagat ttccgtgtac
    27841 agactaatat ttatttcgat accgttgatg aacagctgaa gaaacaaaat tgtggcctac
    27901 ggatccgttt cgttggtcat caagctgaat atacattaaa aacacctgct gaaaaagggc
    27961 gtctcgaaac aacggatacc tttataacag aagaagcaga agcatttatc ttcgaaaaac
    28021 gtctgcctcg ttcaggcgca gtttttcaaa aactttctga tttgaatatc gatcctgctt
    28081 cattaatcca aaccggaaag cttacaacaa aacgagctga attccctatt gaagaaggac
    28141 ttttagctat tgatgaaagc tggggagaca atctacacga ttttgaactg gaactagagg
    28201 taggggatgc atctgtggga aaaattgctt ttataaattt tttaaaacgt ttttctttac
    28261 cttatcgtcc tgctaagaac aagatccaac gtatgcttga agctaaaaat tagaaacctt
    28321 tcattttcac aaggcacttg agttcatttt tttacttttt tctgttaagt tataggatgt
    28381 gaaagaatct agtcactagt tgtatagaat ctattttggg ggagattcat atgatcgaaa
    28441 tctatttatt tgttaatcct ttaggggaat actgtttcga aatggaacaa cagttattac
    28501 aatttatcga agaagagtac gggacaccat caaaagaaaa gatgcaattt cgctttcttc
    28561 ctttggtcaa tttgcagaca attggggatg tcatgcaaca aaaaggaatt tctcagaaca
    28621 atcttgagga acgtaatcac ttattctcaa tgacttattc tgcagcattg gactgcaaag
    28681 cggcgcagtt tcaaggaaag aaaaaaggcc gccgtttttt gatcaaattg caagaagcag
    28741 tcggctgtaa tgacatacca tattctcaag aacttgctga atccatattt gaagaaatcg
    28801 gcggcgattt gaatatgttt aaagaagatc gtcagtctga cttcgttaaa gaagtattct
    28861 tttctgatca aaaaattgct cgggaaatgg gcatcacaaa acacccttct gcagtcgttt
    28921 acaattatgc ctgcgaccgt gattttggtg ttttagtaga agtaaaaggt acaagtagcc
    28981 tttggagaat cacagagctg tgtaaaacaa cagataactc atatcaagtt tttaatggtg
    29041 aacatttaca tacaaatgat tctcagttcc attcaagaac tagccattta cacttgcttt
    29101 ctaattaaaa a
//
LOCUS       DAZKLD010000017        28250 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.017, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000017 DAZKLD010000000
VERSION     DAZKLD010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28250)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28250)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28250
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.017"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            83..1423
                     /locus_tag="ACG8KR_000671"
     CDS             83..1423
                     /locus_tag="ACG8KR_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFQ0200070.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGIILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHAADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFAAYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLIIFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLTLRKGTLW
                     DKGISFFALAVLWLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            1618..2061
                     /locus_tag="ACG8KR_000672"
     CDS             1618..2061
                     /locus_tag="ACG8KR_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HFQ0200071.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINAMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILTSKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(2323..3741)
                     /gene="nhaC"
                     /locus_tag="ACG8KR_000673"
     CDS             complement(2323..3741)
                     /gene="nhaC"
                     /locus_tag="ACG8KR_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HFQ0200072.1"
                     /translation="MEKKGTPSFLEATLVLMVIVLGIAIGVVGLKVSPNITILAAIGM
                     VMLYAVIKKYPTDWLHEGIINGIKPGIIPIFIFILVGSLIAVWIQAGIIPTLMVIGFK
                     LISVQWFVPSVFVVCAIVGSAVGSAFTVMSTIGIAFFGIGTTLGLNPALVVGAIVSGA
                     VFGDKMSPLSESTNLAAAIVDADLFKHIKHMMWSTVPAFVVSFFLFMILGHTSKAASL
                     SSIREVTDILEANFTISIWSLIPLFLMLLCAWKKVPAILTILLNIAVAVGMIFIQNPQ
                     TSLTKLAGVIESGFVSNTGNQQIDSLLSRGGIESMMPTVSLIILTLSLGGLLIEFGLI
                     STVMERVSRKMTNTPKLIFTTLMTSIGVNIFIGEQFLSVILPGNAFKETYEKAGFDPT
                     VLGRTLEDGGTVINYLVPWGIAGSFVASTFGIPTLTYLPFVFFSLLSPVFSMVSAFTG
                     WGISKKFVKPQEAVSKTAQLEQ"
     gene            complement(4145..5368)
                     /locus_tag="ACG8KR_000674"
     CDS             complement(4145..5368)
                     /locus_tag="ACG8KR_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFQ0200073.1"
                     /translation="MTTTLSTNQLKDLIQYRLPEYQALALAIHDHPEVSNYEFYSSAA
                     LIKQLEKEGFWVEKEVAGHRTGFDARYISNKPGPTIAFLAEFDALPGIGHACGHNLFG
                     TYSVLAASIVKQFIDEIGGEIRVYGTPGEEGGENGSAKGSFVREGFFEDVDAALCVHP
                     AFRYGKTTESLANDPVDIKFYGVASHAAAAPEKGVNALEALIQVFNGINALRLQLPKD
                     VNIHGIITDGGVAANVIPDYAAGRFYLRAGNRQTLDKVYEKVENIVRGAALSTGTTFE
                     FGLFQNAVDDVIVTPSFDDLFFQHAKEAGVPDEEIETEQRTSLGSSDVGNVSQVIPTI
                     QPTVAISETYIAGHTEEFKAAAKSEKGLASIAIAAELLANTALDLLREPKLLEKIKQE
                     HQEIVARKKQEGATF"
     gene            complement(5400..6239)
                     /locus_tag="ACG8KR_000675"
     CDS             complement(5400..6239)
                     /locus_tag="ACG8KR_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HFQ0200074.1"
                     /translation="MKKIIRFAVITFVAIIWLAGCSQGKAATTEKKETTVKLGIIGED
                     TDVWDDVKDRLKKENIHLEYVKFTDYNQPNAALADGSIDLNSFQHQFFLDNYNEEHGT
                     DLVSIGNTVNAPLGIYSNTLKDVADIKDGAKVAIPNDVTNGGRALLLLQTAGLIKVDK
                     AKGQAPTVSDITENKKNLDISELDASQTARALSDVDISVINSGVAVDAGFNPASDAIF
                     LEPVDEHARPYVNIIVARKEDEDNKTYQKIVDAYQTEETAKVIEKTSKGSSVPAWETF
                     GRK"
     gene            complement(6256..6936)
                     /locus_tag="ACG8KR_000676"
     CDS             complement(6256..6936)
                     /locus_tag="ACG8KR_000676"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease"
                     /protein_id="HFQ0200075.1"
                     /translation="MTEFFKTYLPNVYLIPDEFIEATKQTLYMSFWTAFIGGIIGIIL
                     GVTLVVTRPNGLLANRLLFEILDKLINIIRSIPFIILLSLLALTTRFLVGTTIGAKAA
                     LVPLIFGVVPFFARQIENALLEVDPGVIEAAEAMGTSPIGIILRVYLLEGLPGIIRVS
                     ALTIINVIGLTTMAGAVGAGGLGNLAISRGYNRFQTDVTIVATLLILVLVFLSQFISN
                     QLIKKVTH"
     gene            complement(6938..8011)
                     /locus_tag="ACG8KR_000677"
     CDS             complement(6938..8011)
                     /locus_tag="ACG8KR_000677"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HFQ0200076.1"
                     /translation="MIELRDISVCFKQKEKEIQAVDSVDLTIEKGDVYGIVGYSGAGK
                     STLVRVMNLLQKPTAGEVIVNQTNLSQLSPKELRKERKAIGMIFQHFNLMESRTIFDN
                     VDFSLKYSGKSKQERRQKVNELLELVGLEEKASAYPKQLSGGQKQRVAIARALANEPK
                     VLLCDEATSALDPKTTLQILALLKKLNRQLGLTIVLITHEMQVVKEICNKVAVMEAGK
                     IIEKGSSIQIFSHPEEELTKDFIRTATHLDQALETISAHAAFADQIANKWLVELSYIG
                     NQTNEPLIAHLYSKYQVTANILYGNVELLQETPLGSLIVTLAGETKQRQKALDYLIKS
                     GVKVTILQKNELDNRIKVIEGGN"
     misc_binding    complement(8070..8308)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     misc_binding    complement(8351..8584)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(8661..9332)
                     /locus_tag="ACG8KR_000678"
     CDS             complement(8661..9332)
                     /locus_tag="ACG8KR_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFQ0200077.1"
                     /translation="MKGAIFDLDGLLVDTERTYRDGWLWGFQQFDIHIPKEIVDAWGG
                     KNWKQSFDILVQAAGSPEKVQEVRAKREEYFYQQLTSGAIQLKPYAKEILTDLKERHM
                     KLGIATTTVTKRATDILDHFQLADYFDTFTFGDEVSENKPSPVPYLTALERTGLEALS
                     AFAVEDSLVGATAASRAGLGVVLIPDSSFVRNYSVEEKEKLNLLAEGKDLRTVIEMLD
                     KKTEK"
     gene            complement(9420..12068)
                     /gene="mgtA"
                     /locus_tag="ACG8KR_000679"
     CDS             complement(9420..12068)
                     /gene="mgtA"
                     /locus_tag="ACG8KR_000679"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015444 - P-type magnesium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HFQ0200078.1"
                     /translation="MEKKKDTQKIYAAFAGKTKQELFELFHASPNGLSEEQIERSRET
                     YGENAISYGKKTPFIIEILKAYITPFTLVLIALGIISFITEYVLAAPGDKDLFGVAII
                     FTMVIVSGTMTLIQSVRSNQAAEKLKSLVKVTAAVKRNGEYTEIPMEEIVCGDLVRLS
                     AGDMIPADMRLLSAKDLFISQAALTGESYPVEKHAKVCEDDQQSETSYENLVFMGSNV
                     ISGSAEGIIVSVGNDTLFGHVAETLSEKPIQSSFELGIHKTSMLLIKFMSLMAPAVIV
                     INGLTKGDWLEAFLFGLSVAVGLTPEMLPMIVTTNLVKGASVMAKKGTVIKNLNAIQN
                     FGAIDVLCTDKTGTLTQDKIILEYHMDCEGNEDDRVLRHAYLNSYYQTGLKNLLDVAI
                     IDEAAETLDTDTINYQKVDEIPFDFERRRMSVVVADPAGKTQMITKGAIEEMLNISKF
                     IDLGGTVAPLTKERKEAVLAKVQSLNEDGLRVIGVAQKTNPSVVGEFSVKDESDMVLI
                     GYLAFLDPPKETTKQALKALKEHGVAVKVLTGDNELVTRSVCRQVGLEINELITGEKI
                     SEMNDHELAQVAEEHEVFVKLNPQQKARLTTALRKNGHTVGFLGDGINDAPAMKVADI
                     GISVDTAVDIAKESADVILLEKDLTILERGLLSGRETFGNIMKYIKATASSNFGNMFS
                     VLIASTFLPFLPMLPLQLLFLNLIYDVSCISLPWDRMDKEYLDEPKKWNASSIGKFMV
                     YFGPTSSIFDITTYLLMYFVICPAVVGGSFHTLDSSQQVIFIALFHAGWFVESLWTQT
                     LVLHALRTPKIPFIQSNATFAMLVITTLGIIVGSVLPFTGFGAELGLMPLPGNYWLWF
                     FITVVAYLALVTAVKKIYIRRFQELL"
     regulatory      complement(12411..12585)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00380"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="The M-box (ykoK) riboswitch appears to function as
                     a genetic 'off' switch that is important for maintaining
                     magnesium homeostasis in bacteria; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="magnesium"
                     /db_xref="RFAM:RF00380"
     gene            12758..13195
                     /gene="ndk"
                     /locus_tag="ACG8KR_000680"
     CDS             12758..13195
                     /gene="ndk"
                     /locus_tag="ACG8KR_000680"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HFQ0200079.1"
                     /translation="MERTLVIIKPDGVRRHLVGSIIQRFEAKGLAIAEMKFATMTPEL
                     AKEHYQHLTERSFFDELIDYMTSGPVVYLVLVGEEVIDIVRKMVGATKGADAVPGTIR
                     GDYALPGTENIIHASDSRDAATKEIARFFPPFDTNKPSEKAAK"
     gene            complement(13217..14083)
                     /locus_tag="ACG8KR_000681"
     CDS             complement(13217..14083)
                     /locus_tag="ACG8KR_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0200080.1"
                     /translation="MYDGELIKKLRVNKKLTQTQLAEGICSKTSLVGIETNSVKKVSF
                     GTLKSFLERMNMTLAEYEWIRNQLDEPKKLKKKRRMLDRIQEDSFDPYKEIANNRRHY
                     KKTEDLYYLVLNLHMSKKTEVSETEFEFLRYECRIIEEYLMQVPEYGQFEVSILADFP
                     WIFSDRFIENNYLKIKKRMRRIAQLNNHEQYLFTFLTNLTLFYIENGRLKKARSVNED
                     MKRSLAHKERSTVIYETLMADYYQRLISAALGEEKSENHQEFFAVIEYTLGKNQRNSL
                     EKKLLAIAKPSS"
     gene            complement(14317..16395)
                     /locus_tag="ACG8KR_000682"
     CDS             complement(14317..16395)
                     /locus_tag="ACG8KR_000682"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFQ0200081.1"
                     /translation="MTQLKKLCQNRLAIVLTAVFFFWLKTIIAYYADFSLGVEGTIQY
                     FILWINPIATTLLFFGLSLYIKKPKPALAILLIIDILNTLLLYLNIIFYREFTDFITV
                     KSVLGFSKVSQGLSGSSFSLMKPHDVIYWLDIAVFIGLLVWLKVKKIPIKSNPVSKPM
                     AIAVTCLSGLIFSGNLALSEANRPQLLQRTFDRSYIVKYLGIDAYTIYDGIKTGMTSS
                     VRAHASSNGIDEVLDYTKKHYAEPNPETFGIAKGKNVIVLHLESFQQFLINMKVDGQE
                     VTPFLNSIFQNQATISFDNFFHEVGQGKTSDAENMLETGTFGLPQGSLFTELGSDNVF
                     QAAPATLGQTEGYTSAVFHGNVASFWNRDHVYKNLGYDNFFDRSYFDESDETLGYGIL
                     DKDLFRESAQYLEHLQQPFYTKFLSVTNHTPYYTDDKNFDFPSLNTGNSTVDNYVRTA
                     HYLDQSLEQFFTYLKKSGIYQNSIFVIYGDHFGISNTDNKDLASALGKDPDTWDEFDN
                     AQMQRVPLMIHMPGYTKGTVNHEYGGEIDVLPTLLHLLGIDDKDYLHFGTDLLSSQHD
                     QVVAFRNGNFVTPKYTVLGGKAYNNQTGEIIDTKAAGIDEEISKDQEKVKTALSLSDK
                     LNQENLLRFYVPNGFETIDPKKYDYKNEAERNEAIEKQKGEHSTSVFSKNGNKTTTIL
                     YPVTGTNAEQ"
     gene            16888..18105
                     /locus_tag="ACG8KR_000683"
     CDS             16888..18105
                     /locus_tag="ACG8KR_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFQ0200082.1"
                     /translation="MPYILALDVSMGHSYSVLYKDDTCLFEDEIEHTQRGFHALLEEI
                     QHLPERPLIVFEATGIYSRPVEKFFQDHHFSYCLLNPLEAKKQMEENTLRSWKTDKSD
                     AHRLAQTHLKNQRQPKEVQSNFYLEMRDLARFYQEIEKKITRLRMDLHNCLQLTFPEL
                     EQFFSNRLTPYALTLIRLFPHPDFVLASTRTKIKNKLINETREKISANRAEQKADQII
                     HYAQCAYPAVEKDSIHCQKTIYYAELLQDLLEQKKALATQMIKKAEASPCFLLYQTFP
                     GIGALTAALLLGELGDITRFKTHKQLNAFVGIDIRRYHSGKYTGQDRINKRGNPKARK
                     IIFFTIRNMIRQQRAAPNHIVDYYYKLKKQPIPKKDKVATVACMNKLLKCMHAMVRAH
                     TEYDYAYAVSVDH"
     gene            complement(18564..19187)
                     /locus_tag="ACG8KR_000684"
     CDS             complement(18564..19187)
                     /locus_tag="ACG8KR_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0200083.1"
                     /translation="MRKKRPFLLFWLFGFFLLLILSAAFSGKMTADLAQKETEKIVAS
                     KESVDNFKKEFSTYWWEEYKLTANYEKGELDIYLPKGKEENQFINIDDVLSAISLRQY
                     QFKDKHLVVLLLTHDGDKVARLKNGKVVTAYSEKVDKKVVADRLAEWKKTNEQVKKTT
                     ASSQKNESTELSPSDSGSGQTITGNEENQASIITNESSEGQYQTYGQ"
     gene            complement(19343..20740)
                     /locus_tag="ACG8KR_000685"
     CDS             complement(19343..20740)
                     /locus_tag="ACG8KR_000685"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFQ0200084.1"
                     /translation="MKRYQFPKNFWWGSAASGPQTEGRISGDGKGENIFDYWYKNEPQ
                     KFFDQVGPEKTSQVYTKYQEDVQLMKQTGHNSFRTSIQWSRLIPDESGTINEEAVRFY
                     HHYFDELIENGIEPFVNLYHFDMPMYLQEKGGWLNRETVEAYEKYAKSCFQLFGSKVK
                     KWFTHNEPIVPVEAGYLYRWHYPEESDMKKAIQVGYHEALASALAIKAYHEMDQSGVI
                     GIILNLTPSYPRDESNPEDVKAARIADAFFNRSFLDPAVKGEFPSELIERIKELDLMP
                     EIHPDDLTIIKENTVDLLGINYYQPRRIKAKETPIDTKNGPMPEDYFDNYEMPGRKMN
                     PYRGWEIYEKGIYDILINVRDHYGNIDCFISENGMGVENEARFIKEDGMIHDDYRIEF
                     VQSHLKYVHQAIQEGANCLGYHMWTCMDNWSWTNAYKNRYGFISVDLAQDGKRTIKKS
                     GHWFKDVSDRNGFEA"
     gene            complement(20762..21733)
                     /locus_tag="ACG8KR_000686"
     CDS             complement(20762..21733)
                     /locus_tag="ACG8KR_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0200085.1"
                     /translation="MASIRDVAKMAGVSVGTVSRYLNGQQLKEKNMEKISEAISALDY
                     KENIIAKGLKNNRSFSIGLLINGISNRFGSEVVSGIERVAEENGYSLLLSGFADQPEK
                     IEQKIEYLMKHAIDGLIVFLSEEEWSGFEMLTKMSIPVVALNCPGGPAGIDTIVVNDR
                     ESVAKVIAHHGGQGHKTIGFIAAAQTDYVARERLAGAKEAVQKDPSIHLEIFTGDYSR
                     KSGYYGAKALLEKGATAIFVSNYNMSIGAIECFNQEGVRIGKDIAFSHYDYLDKNDLS
                     ILPKITITPPTDKIGERAAEQLLKRIKKEEEENEKVIVMENIIHGIN"
     gene            complement(21740..24040)
                     /locus_tag="ACG8KR_000687"
     CDS             complement(21740..24040)
                     /locus_tag="ACG8KR_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HFQ0200086.1"
                     /translation="MYDYPYQNPKLTIRQRVEDLLSRMNIAEKVGQVNQHLYGWECYE
                     KNGDKIELTEKLKEHVKWGGGLGALYGLFRSDPWSKTDYKNGIPAKESWKVANLVQEY
                     VLQHSRWKIPVLLVEECPHGHQGLDGISYPTNIGRGNTFNVALLEQSSRLMAKELAAK
                     GVHLALVSTLDLAKDPRWGRTEECFGEDPILSARFSEAVVRGFQGEIISEKVSFLDQT
                     VEEINKKKDQIGVVLKHCIAQGEALGGHNSGTVTIGRREFSDIYGPLLKSTRNAVGVM
                     AAYNDIDGIPCHINRALFEQTLRKEIGYQGIVMADGVALDRLSDVFTDKKTAAAYALA
                     AGIDLSLWDETYTKIAEAIDNQVVDEKLLDQAVRRVLSVKFLLGLFEQPFANDPKEYW
                     DHLMEESEHLNLETAKESITLLKNDGILPLDQQVKNIAVIGPNAHDVYHLLGDYSAPQ
                     TEDRLKKKIVQEIKTEFPQSKVSYAQGCEVRNQEDQEEKLLEAVTLAQNSDVIIAVLG
                     GSSARNFDMEFLRNGAVSSKGINMDSGENVDVASLSLGGYQEQLIEQLYQLGKPIITI
                     LVQGRPYDLMKIESFTNAIATAWFPGQEGGKAIAQMISGRNNPSGRLSLSYPRNSRQL
                     PVYYYQRAASKQENYYDLSGSPFYPFGHGLSYTTFEYSQMEVKTDIDKVIISIKVKNT
                     GSVSGKTSTLVYVRLIDGVVIQRTKLLKAFRKDELRSQEERLLVFELTSEDFSYMDID
                     DKRHYAKKAVIMVEQQEKEIKCNWQE"
     gene            complement(24042..25367)
                     /locus_tag="ACG8KR_000688"
     CDS             complement(24042..25367)
                     /locus_tag="ACG8KR_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFQ0200087.1"
                     /translation="MKVVDRLSEKMLPIVSKMSSQRHLLAIRDSFIVTMPLVMAASVM
                     VLLNALIFSNATVQRILDLSPLAELASIVNNGTMSILAIIVCYNIGWNLATHYIQTGV
                     IDDPSFSPVHAGILSTAVMFILMSLSSTVTLMDGNTAEATGVFLQSLTSSSGLATAMI
                     AALVSTELFIKLAKMKSFKIRMPEGVPPAVAASFNSLVPEIVVILFFSVVVFTLNHTT
                     GLNVPQLIELAIQTPLKAFVLSVPGILFLQFFSDFLWVFGMHGSSILAPIRQAPLLQS
                     IQENMDAFSVGKEIPNIITEPFTNAFGLIGGGGCILPLVIAILWASKRQEQRSIAKFG
                     LTTCLFNITEPIMFGLPVVMNPIYMIPCAIIPSINLVIAYAATSLGIISKTVAAAPWI
                     TPPVIQSFIATGGDIRAAVLTVILIILDVFLFLPFVLAANKAKLAEGGY"
     gene            25598..26023
                     /locus_tag="ACG8KR_000689"
     CDS             25598..26023
                     /locus_tag="ACG8KR_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0200088.1"
                     /translation="MKKLIVLFILSAGLLTLTGCDLFGGSDQSSDETTDSSVLKAEKA
                     DITNSVYTLKVRKDMDLSAGQLTFTDKKMEWTRTYQTQGADSSESSEEKTVLTDIKIT
                     TKDDEYIISGKENKKEATVTFTKIGNYRLKDKDGNIYSL"
     gene            complement(26521..27222)
                     /locus_tag="ACG8KR_000690"
     CDS             complement(26521..27222)
                     /locus_tag="ACG8KR_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HFQ0200089.1"
                     /translation="MSVILISGTIGAGKSSLTDMLAKEIDSKPFYENVEDNEVLPLFY
                     SNPEQYAFLLQIFFLNKRFLAMKNALVNDDNVLDRSIYEDSLLFHLNADLGRVTDIEV
                     QQYDSLLDTMLNELDDVAPKKRPDLMVHIKVSLDTMLERIKKRGRDYEQLESDETLYT
                     YYETLNTRYNQWYEDFDVCPKIQVDGDTFDFVENPEDAKKVIRQIQEKLAELEGKPAG
                     KYFTDNQHAKGQELG"
     gene            complement(27238..27678)
                     /locus_tag="ACG8KR_000691"
     CDS             complement(27238..27678)
                     /locus_tag="ACG8KR_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside 2-deoxyribosyltransferase"
                     /protein_id="HFQ0200090.1"
                     /translation="MSQIYLAGPFFSEEQIDRVSRIEKALEENKTITSFYSPRHHQES
                     NYELFSAGWAQEVYEKDMEELTNAEFVLAILDFEHQTIDPGTAYELGVATMLKKPMII
                     VQEETVPTNLMITQSLHTYLKSDQAVREYDFEKLPVETYVGEYL"
     gene            complement(27678..>28250)
                     /locus_tag="ACG8KR_000692"
     CDS             complement(27678..>28250)
                     /locus_tag="ACG8KR_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009691 - cytokinin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HFQ0200091.1"
                     /translation="KLTVFCGSRFGNKESYKFIAQTLGKYMAQEEIELVYGGSYSGIM
                     GVISKTVLENNGKVTGIYPNGLFETELPGKDVTTFIPTETIDERKVLLFEKGDAVLVF
                     PGGLGTLEEFSQLLSWIAIGLTPDKPIGILNIGGYYSGLQQLLETFAKEDFMDKKWLD
                     QVIFSNNPLELVNELRAVSIKNQLLLKEAK"
BASE COUNT         8186 a         5964 c         4872 g         9228 t
ORIGIN      
        1 tcattattat tattactatt tattcttact atttattctt actatgcatt ttgattgtga
       61 atttaaaaga aagaggttat ttatggaaga taaaaaattg aaaaaacaac ttagctcccg
      121 acatatcacc atgctagctt taggtggagc gattggtgca ggtctattta aaggaagtgg
      181 cgaagcgatt ggaatcgccg gcccttctgt attgatcgct tttttgattg gcggcataat
      241 tttatatatc gtgatgaaag gtctgggaaa aattgtttta agtggtggtg atacgcacca
      301 cggattatct ggattgattc gtccatatct aggtgctcat gctgccgatt ttaccgattg
      361 ggtctactgg tccatgtgga ttatcaatat cattgctgaa gctgttgcag ctgcttcctt
      421 tttacagttg tggttccccc acatcccagc ttgggtattt gtttttatgt tggctgtttt
      481 aactactatc atcaatcttt attcggtacg attattcgca gagacagaat attggctggc
      541 ctttgctaaa atcagtgtca ttatcctatt gatcattttt gctgcttatc ttgtcggtca
      601 gcaaatgttg ggtactggtg ttttccctac ccttcaacaa ttaactgatc atggtggttt
      661 tactccccac ggtatgaagg gaatcatcag ttctttgctt gtagttatct attcttacgg
      721 cggttctgag ttgattgcta tcacagtaag cgaagcagat gatcctaaac gtgcgattcc
      781 taaagcaatc aaaggcgtca tgggaagaat catttccttt tacattattc ctttattttt
      841 gcttttgatt atttttcctt ggaatacttt agcaggcacg aacgttagtc cttttgtgat
      901 ggtatttgaa aaaatgaata ttccatttgc ggcagatatc gtcaatttcg tgattgttct
      961 tgctctgttc tcgtctatca attcaggtgt atatgcttct tcgagaattt tgttcttccg
     1021 cttgaaagat cggaaaggtt cctctagaaa actagctgtt ttaaacaagc atcaagtacc
     1081 gcaacgtgct gtgctgtttt gtaccagtgt tctttatcta ggtgtggctc tttcttattt
     1141 tgtcggtgac aaattatttg gctatctggc aggttctctt tcctatacag tattattgat
     1201 ctggatcatt atcagtgctg cgtcctttgt actgacacta aggaaaggaa cgttatggga
     1261 caaaggaatc agcttttttg cattagcagt cttatggttg attttccttg gtatcctatt
     1321 cacgaactcc attggggtaa ctgtattgac cggtttgctt tacctgttca tttatttcag
     1381 ttatcaaaaa aagaatgatg ctttcgtctt gacgaatgaa tgaaaccaat agagaatttt
     1441 attctattta tattgaatat ctggagctat tgaaaaaaag gagctgcgac aaaaattgtt
     1501 gcagttcttt tttccgaata aacggtgttc aaaaaccatc tgtgcagatt aattgattgt
     1561 tctccacgct catgacatga tagaagaaag gaatgaagaa aaaggagttt gttttaaatg
     1621 aaaaagtatt ggaagttttt gttgatgatt ggtgtttcga cggtagtcat gtttggtatg
     1681 atgtatttga atgtttatgc gcttgatcat atcttcttca gtcgcacacg tgtattcatg
     1741 gcgttgatga tgggtgcagt catggcagtc atcatgttgt tgttcatgtg gaaaatgtat
     1801 gaaaacaaaa agataaatgc tatgatttta gccgctagtc ttcttgtttt tgccggttcc
     1861 ttatttatgg tacgaagcca aacaactgtc aacgatacgg cgtggatgaa ggcgatgatc
     1921 cctcatcact ctattgctat cttgacaagt aaacgagctg atttaaaaga tcctgaagta
     1981 aaaaaattag cggaagaaat cattcaggca caagaagaag aaatcgacaa aatgaaaaaa
     2041 atgatcaaag aaatggaatg accattatcc atccaaataa aaaaagagga tgttccagaa
     2101 gtgatcaact tcaagaaata agaagaagct acttctggaa caccgtgaat cagaggttgt
     2161 gaaaaagctg ccctgcatca agtaataaga acagccaaac aaaaatagct tctcatattt
     2221 ttcgtttggt taggcttatt accgcaaatg caagcttttg aacaccgttt attcgtaaaa
     2281 gggagtgtga caaaagtctt gtcacactcc ctttttacgt ttttattgtt ctaattgcgc
     2341 tgtcttgctt actgcttctt gcggtttcac aaatttcttt gagatccccc aacctgtaaa
     2401 ggcactgacc atcgaaaaca caggtgaaag caaactgaaa aacacgaatg gcaagtaagt
     2461 gagtgtagga atgccaaatg tacttgctac gaaacttccg gcgattcccc aaggcactaa
     2521 gtagttgatt actgttccac catcttctag tgttctccct aatacagttg gatcaaatcc
     2581 tgctttttcg taagtttctt taaatgcatt accaggcagg ataacagata gaaattgttc
     2641 tccaataaag atatttactc cgatgcttgt catcaatgtt gtaaaaatca attttggggt
     2701 gttcgtcatc tttcttgata ctcgttccat aacggttgag atcaacccaa attcgatcaa
     2761 caaaccacct aatgacaatg ttagaataat caatgacaca gttggcatca tgctttcgat
     2821 tcctccacgg ctaagtagtg aatcgatttg ttggtttcca gtatttgaaa caaatcccga
     2881 ttcgatgacc cctgccaact tcgttaagga agtttgtggg ttttgaataa agatcattcc
     2941 tactgctaca gcaatattca acaaaatcgt caaaattgcc ggtacctttt tccatgcaca
     3001 caagagcatt aaaaatagag gaatcagtga ccagatcgaa atagtaaagt ttgcttctaa
     3061 aatatcagtc acttcacgaa tagatgaaag acttgcggct ttacttgtgt gtcctaaaat
     3121 catgaataaa aagaaagaaa caacaaatgc cggaacagtt gaccacatca tatgcttgat
     3181 gtgtttgaac aaatcggcat caacaatagc tgctgctaaa tttgttgatt cggataatgg
     3241 agacatcttg tctccaaaaa cggcacctga aacaatggct ccaacaacaa gggctggatt
     3301 tagtcctagt gttgtcccaa taccgaaaaa tgcaatccca atcgttgaca taacagtaaa
     3361 ggcactccct acagcgctac ctacgattgc acaaacaaca aatacagaag ggacgaacca
     3421 ctggacacta atcaatttaa atccaatgac catgagggtt ggaataattc ctgcttgtat
     3481 ccatacagca atcaatgaac cgactaaaat aaaaataaag attggaatga tccccggttt
     3541 gatgccattg atgatccctt cgtgcagcca atctgtcggg tattttttta tcactgcgta
     3601 caacatgacc attccgatag ctgccaaaat cgtgatatta ggtgaaacct ttaatccgac
     3661 aacgccaatc gcaatgccta aaacaatgac catcaataca agtgttgctt ctaaaaaact
     3721 tggtgttcct tttttttcca tgataatctc tcctttttta gttgttctta tgttctaaaa
     3781 aagttaagat atcggattac tccgccacaa ttcatccgcg attcctcctt tataaaaata
     3841 aaaagacctg ctttcaacta aggacgaatg actccgcggt accaccttaa ttaaaaacag
     3901 atctgttttt cactttcaaa cgattattca aatgtatgga ctttccatgt aattcgtcaa
     3961 ttaggcttga tcggttcaca gcgaccaccg actttctgaa caaaacacct aaccactact
     4021 gcgtgaaaag ttgacttgta tgaagtttac cttccataag atcatttgtc aatgaaaaag
     4081 aaaatatttt ttcatttttc tgaaaattat ttttataact ttcgcgcaca actttatctt
     4141 tttactagaa agtagcgccc tcttgttttt ttcttgctac tatttcttga tgttcttgtt
     4201 tgattttttc taatagcttg ggttctctca ataaatctag agctgtattg gcaagtaatt
     4261 ccgctgcaat cgcaatagaa gcaagtcctt tttcactttt agcagcagct ttgaattctt
     4321 ctgtatgtcc ggcaatatat gtctcagaga ttgctacagt aggctgtatc gttggaataa
     4381 cttggctaac atttcctaca tcagaagaac ctagacttgt tctttgttcc gtttctattt
     4441 cttcatcagg aacccctgct tcttttgcat gttggaagaa taagtcatcg aaagaaggcg
     4501 ttacgatgac atcatcgact gcattttgga acaatccaaa ctcaaaagtc gtacccgtgc
     4561 ttaatgcagc acctcgtacg atattttcca ctttttcata tactttgtct aatgtttgtc
     4621 gatttcctgc acgtaaatag aaacgtccag cagcgtagtc tggtatgaca ttggccgcca
     4681 cgcctccatc tgtaatgatt ccatggatat tgacatcttt tggcaattgc aagcgtagag
     4741 cgttgattcc gttaaacact tggatcaagg cttccaaagc atttactcct ttttcaggtg
     4801 ctgctgctgc atgagaagcg acaccgtaaa atttgatatc gactggatca tttgctaaag
     4861 attctgtcgt tttcccataa cgaaaagctg gatggacaca taatgctgca tcgacatcct
     4921 caaaaaaacc ttcccgaacg aagcttccct tagctgatcc attttcgcct ccttcttccc
     4981 ccggagtacc atacacacga atttctccgc caatctcatc aatgaattgt ttcactatgc
     5041 tggctgctaa tacggagtaa gtaccaaata aattatgtcc gcatgcatga ccaatacctg
     5101 gtaaagcatc aaattctgcc aaaaaggcaa ttgtcggacc tggtttattg gaaatataac
     5161 gtgcatcaaa gcctgttcgg tgcccagcta cttctttttc tacccagaac ccttcttttt
     5221 ccaattgttt gattaaagct gctgaggaat aaaattcata attacttact tcaggatggt
     5281 cgtggatagc tagtgcaagt gcttgatatt ctggtaaacg gtattgaatc agatccttca
     5341 attgatttgt tgagagtgtg gttgtcataa aaatttcctc ctatgctagt cgttttttct
     5401 tattttctcc cgaatgtttc ccacgcaggt acgcttgaac cttttgatgt tttttcgatt
     5461 actttggctg tttcttctgt ttgataggca tctactattt tttgatacgt tttattgtct
     5521 tcatcttctt ttcttgcaac gatgatattg acataaggac gtgcatgctc atcgactggt
     5581 tctaagaaga ttgcatcaga agcgggatta aatccagcat ctacagctac accgctattg
     5641 atgactgaaa tatctacatc tgacaaagca cgtgctgttt gggaagcatc taactcagaa
     5701 atgtctagat tttttttatt ctcggtaata tcagaaacgg taggcgcttg gccttttgcc
     5761 ttgtccactt tgatcaatcc agcagtttgc aataacagaa gagctctgcc gccattcgtg
     5821 acatcattag ggattgcaac ttttgctcca tctttgatat cagccacatc ttttaacgta
     5881 ttggaataaa ttcccaacgg tgcattaacc gtattcccaa tcgatactaa atcagttccg
     5941 tgttcttcat tgtaattgtc taaaaagaat tgatgttgga aggaattcaa atcgatcgat
     6001 ccatctgcta aagccgcatt tggctgattg taatctgtaa atttcacata ttccaaatgg
     6061 atgttttcct ttttcaaacg gtctttcaca tcgtcccata catctgtatc ttccccaatg
     6121 atccctagtt ttacggtcgt ttcttttttt tcagttgtag ctgcttttcc ttggctgcat
     6181 cctgccaacc aaataattgc tacaaatgta atgactgcaa aacgaataat ttttttcatt
     6241 ttttctcctc cattttcaat gtgtgacttt tttaatcagt tgattactga taaattgact
     6301 caaaaaaact aagaccagaa tcaataatgt agcgacaatc gttacatctg tttggaatcg
     6361 attatatcct ctcgatatag caagattccc taatcctccg gcacctactg ctccagccat
     6421 ggtagtcagt ccgatcacat tgatgatcgt caacgctgag acccgaatga taccaggtaa
     6481 cccttctaac aaataaacac gtaaaatgat gccgattggg ctcgtgccca tcgcttctgc
     6541 tgcttcgatc accccaggat caacttctag cagtgcattc tcgatttgac gggcaaaaaa
     6601 cgggacaacg ccaaaaatca aagggactaa agctgctttg gcaccaatcg tcgttccaac
     6661 taagaaacga gtagtcaaag ctaaaagaga aagcaagata atgaaaggga tggaacgaat
     6721 gatattgatc aacttatcaa gtatttcgaa cagcagcctg ttagctagta atccatttgg
     6781 acgtgtcact actaacgtca cacctaagat gattccgatg atcccaccaa tgaatgccgt
     6841 ccaaaaagac atatatagtg tttgctttgt cgcttcgatg aattcatccg gaatcaaata
     6901 gacattcggc aaatatgttt taaaaaattc agtcatttta gtttcctcct tcgatgactt
     6961 tgatacggtt atctaattca tttttttgta aaatagttac ttttacacca gatttaatca
     7021 aataatccaa tgctttttgc cgctgtttgg tttctccagc taaagtaacg atcaaacttc
     7081 ctaagggtgt ttcttgtaat agctctacat taccgtataa gatattcgct gttacttggt
     7141 acttgctgta aagatgagcg attagaggtt cgttcgtctg gttgccgata taacttaatt
     7201 ccaccaacca tttatttgca atctgatcgg caaaagcagc atgggcactg attgtttcca
     7261 atgcttggtc aaggtgtgtc gctgtccgaa taaaatcttt agtcaattct tcttcaggat
     7321 gactgaaaat ctggatgctg ctaccttttt caatgatttt ccccgcttcc ataaccgcta
     7381 ccttgttgca gatttctttg acaacttgca tctcatgagt gatcaggacg attgttagac
     7441 ctagctgacg atttagtttt tttaacagtg caaggatctg aagggtcgtt ttcggatcaa
     7501 gtgcactagt tgcttcatcg cataacaaca ctttaggttc atttgccaga gctcgtgcga
     7561 ttgccactcg ttgtttttgc ccccccgaca gttgtttagg atacgcagat gctttttctt
     7621 ccagaccgac aagttccagt agttcattca ctttttgacg acgttcctgt tttgattttc
     7681 cagaatactt tagagaaaaa tctacgttgt caaaaatcgt tcgactttcc attagattga
     7741 aatgctgaaa gatcataccg atggcctttc tttcttttcg caattctttt ggagagagct
     7801 ggcttaaatt cgtctgattg acgatgactt ctcctgctgt tggtttctgc aacaaattca
     7861 tcacgcggac taacgtactc tttcctgcac ctgagtaacc gacgatccca taaacatccc
     7921 ctttttcaat cgttaaatct acagaatcta ctgcctgtat ctctttttct ttttgtttga
     7981 aacatacaga aatatctcgc aattcaatca ttaacattcc tccttgtcta taaaaaaagc
     8041 atcctcaaga aaatgaaaat agtcattttc ttgaggacgc ctttttgcgt gttaccacct
     8101 catgttcaag ctgtgttcac acacaactcc tcttcaagta ccatcatact ctagcaagat
     8161 atcggtcgca gccgtcaccg ccttacatct atgctcggga tgaattgctc gatggccatt
     8221 ttccaatagt cgttccttgc ttcttctcag ctaccgaagc tctctgtcaa aggttcttct
     8281 atcgtactta ccatttcaat gcaaataata ttcaaaaaaa aaatcctttt actaacataa
     8341 actatgtcag taaaaggacg acgtatcgtg gtaccacctt ttttcggaag tttccttccc
     8401 tcaagcccaa catctcaaaa agagagattg gcgttcgttt taacggtcga acatttccga
     8461 atttgcctac ttatcgacaa attattctcc aaggccattt tcagcttttc ttacttgact
     8521 cttcacacca gccagagcct ctctgcaaag tttccaagct tactttcctt ctcaacgaaa
     8581 tcaatatttt gttgttggga ataatctaca ggaggaaaac tgaaaagtca agcatgaaat
     8641 ccaaattatt ttttctatat ttatttttct gtttttttat ctaacatttc aataacagtt
     8701 cgaagatctt ttccttccgc aagaaggttc aatttttcct tctcttcgac gctataattt
     8761 cgaacaaaac tggaatcggg aatcaaaaca acgcctagtc ctgcacgtga agcggctgtc
     8821 gctcctacta aagaatcttc cactgcaaaa gctgaaagag cttctaatcc tgttcgttcc
     8881 aaagctgtca gataagggac tggtgaaggc ttgttctccg atacttcatc tccgaacgta
     8941 aacgtatcaa agtaatcggc taattgaaag tgatccaaga tatctgttgc tcttttcgta
     9001 acagttgtcg tagcaatccc tagtttcata tgacgttctt ttaagtctgt caatatttct
     9061 tttgcatagg gtttcaattg gatagctcca cttgtcagct gttgataaaa atattcttca
     9121 cgctttgctc ggacttcctg cactttttca ggactgcctg ctgcctgcac taaaatatca
     9181 aaactttgtt tccagttttt tccgccccaa gcgtcaacga tttctttcgg gatatgaata
     9241 tcaaattgct ggaatcccca aagccaacca tcccgatacg ttctttccgt atccactaac
     9301 aaaccatcta aatcaaatat tgcacctttc atctatacat taatcctttc caaaacaata
     9361 cacctagttt agcatgactg atccaaaatg aacagaagca tagagcccag ccgcatcact
     9421 tataagagtt cttggaatcg acggatatag attttcttca cagctgtcac aagcgccaga
     9481 taagcgacga ctgttataaa aaaccataac cagtaattgc cagggagcgg catcaagcct
     9541 aactcagcac caaatcctgt gaatggaagg actgaaccga caatgattcc taatgtagtg
     9601 ataaccagca tggcaaatgt tgcattgctt tggataaatg gtattttcgg tgtacgcaat
     9661 gcatggagta ctaatgtttg tgtccacagg gattcgacaa accatcctgc atggaataat
     9721 gcgataaaaa tcacttgttg tgagctatcc aatgtatgga aacttccacc aactacagca
     9781 ggacaaataa caaaatacat caacaagtaa gtagtgatat caaaaatcga acttgttgga
     9841 ccaaaataaa ccatgaattt tccaatacta gatgcattcc atttctttgg ctcatccaaa
     9901 tattctttat ccatccgatc ccatggtaag gaaatgcaag aaacgtcata gatcagattc
     9961 aaaaatagaa gttgcaaagg caacattggt aaaaatggta agaacgtact ggcaatcagt
    10021 acggaaaaca tattgccaaa attagaactt gctgttgcct tgatatattt catgatgttt
    10081 ccaaaagttt ctcttccaga aagaaggcct ctttccaaaa tcgtcaaatc tttttctaaa
    10141 aggatcacgt ctgctgattc ttttgcgata tccacagctg tatccacgga aataccgata
    10201 tcagccactt tcattgctgg agcatcattg ataccatctc ctaaaaatcc gacagtatgc
    10261 ccatttttac gtaaagctgt cgtcaatcgt gctttttgct gaggattcaa ttttacgaat
    10321 acttcgtgtt cttcagctac ttgcgctaat tcatgatcat tcatttccga gattttctca
    10381 ccagtaatca attcattgat ttctaggcct acttgacggc acacagaccg tgtgactaat
    10441 tcattgtccc cagtcagaac ttttacagct acaccatgtt cttttaaagc tttcaacgct
    10501 tgttttgttg tttcttttgg tggatcaagg aaagcaagat aaccaatcag aaccatgtct
    10561 gactcatctt tgactgaaaa ttctccaaca acgcttggat tggtcttttg tgcgacacca
    10621 atcactcgta agccgtcttc attaagtgat tgtacttttg caagcaccgc ttcttttcgt
    10681 tcttttgtta atggtgcgac agtaccacca agatcaataa atttagaaat gttcaacatt
    10741 tcttcaatcg cgccttttgt gatcatctgt gttttgccag ccggatcagc gactacaaca
    10801 ctcatacggc gacgttcaaa atcaaaagga atttcatcta ctttttgata attgattgta
    10861 tctgtatcta aagtttctgc tgcttcatca atgatagcca catctaacaa atttttcagc
    10921 cctgtttgat agtagctgtt tagataagca tggcgcaaaa cacgatcatc ttcatttcct
    10981 tcacaatcca tatgatattc aagaatgatt ttgtcttgtg tcaatgttcc cgttttatcc
    11041 gtacataaca catcgatcgc accaaaattc tggatagcat ttaaattttt gatgactgtt
    11101 ccttttttgg ccatcacact tgctcctttg accaaattcg tcgtaacgat cattggaagc
    11161 atttcaggcg ttaaaccaac tgccacagat aatccaaaaa gaaaagcttc taaccaatct
    11221 ccttttgtaa gcccattgat aacgataact gctggagcca tcaatgacat aaatttgatc
    11281 agcagcatgg atgttttgtg gatgcctaat tcaaaacttg attggatggg tttttcagat
    11341 agcgtttctg ctacatgccc aaatagtgtg tcatttccga cagaaacaat gattccttcc
    11401 gcgcttccac tgatgacatt gcttcccata aagacaagat tttcataact tgtttcactt
    11461 tgctgatcgt cttcacatac tttcgcatgc ttttctactg gatagctttc ccctgtcaat
    11521 gctgcttgag aaatgaatag atcctttgcg gacaataaac gcatatccgc tggtatcata
    11581 tctcctgctg aaagccgaac aagatctcca cagacgattt cttccatagg aatctctgta
    11641 tactcgccat ttcgtttgac agctgccgtt actttcacaa gtgatttcag tttttcagct
    11701 gcttgattag aacgcacaga ctggatcaac gtcatcgtcc cactgacgat aaccatagtg
    11761 aagataatgg ctacaccaaa gagatcttta tctcctggag cggctaatac gtattccgta
    11821 atgaatgaaa taattcctaa tgcaatcaac actaatgtaa agggtgtgat gtatgctttc
    11881 aatatttcta taatgaatgg tgtttttttg ccatatgaaa tcgcattttc accataagtt
    11941 tctcttgaac gctcgatttg ttcttctgac aatccatttg gtgatgcatg gaacaattca
    12001 aataattctt gcttcgtttt tccagcaaaa gctgcataga ttttttgtgt atctttcttt
    12061 ttttccatct cgatctctcc tttgtgtcca aaagacagct gttctcgctg tttttggtct
    12121 tttgctattc atcggatcga tagagaaaat gctgaaaggg gctgagaaca gatagataat
    12181 agccctggcc tttcctctaa atccttgttt ttcgaatgac tgtcatccat ttgttctcat
    12241 ctcctttact aagtaatttt aattcatgac ttccaatcat ttttcgaata gttattttcc
    12301 caactcgctc acctcccttt ttgttttttc acctatggga tttgcggcaa caaaaaaacc
    12361 tctcgccaaa tactcagcga aaggtttgaa aaagcaaatc aaaaaaagct tctcagatct
    12421 catcgttgag ttttgacact gtacaacgta aaaagcagat atctgctttt agctacctta
    12481 tgaaaagcct tatctcgaca attcctgttc tacccattgg cgtctctcga cgtttttggg
    12541 cagtagccta tgtttgtata ggagcctcac ctaacaaatc ctattcggtt ttccctcgtt
    12601 aatataacaa ctaaaaaaga ggatgtcaaa tcaattttcc atgtttttca tactttttta
    12661 aaatacacgc gttatatttc ctgtctcacg cagttttatt tgtctcattt tccacctccc
    12721 gctataatag aattatagta ggaaacgagg tgctgttatg gaacgaacac ttgttattat
    12781 caagccagac ggcgtaagac gccatttggt cggaagcatc atccaacgtt ttgaagcaaa
    12841 gggactggcc attgctgaaa tgaaatttgc aacgatgaca cctgaactag caaaagaaca
    12901 ttatcagcac ttgactgaac gtagtttttt tgatgagctt atcgattaca tgacttctgg
    12961 tccggtcgtt tatcttgtgc tggtcggcga ggaagtgatc gatatcgtcc gtaagatggt
    13021 cggagcaaca aaaggggcgg atgccgttcc aggtacgatc cgaggagatt atgcactgcc
    13081 ggggactgaa aacatcatcc atgcttctga ttcccgtgat gctgcaacaa aagagatcgc
    13141 ccgctttttc ccgccattcg atacaaacaa accgtctgaa aaagcggcga agtgatacaa
    13201 atcctaaact gttggtctaa ctgctgggtt tagcaatggc cagcagtttt ttctctaaag
    13261 aattcctttg attcttccct aatgtatatt cgatcactgc gaaaaactct tggtgatttt
    13321 ccgatttttc ttcccctaaa gcagcagaaa tcagtcgttg gtaataatcc gccatcaatg
    13381 tttcataaat cactgtactt ctttctttat gcgctaaact ccgtttcata tcttcattca
    13441 cgctacgtgc cttttttaag cgaccatttt ctatataaaa caaagtcaag ttcgtcaaaa
    13501 aagtaaagag atactgctcg tgattattca gttgcgcgat ccgtcgcatt ctttttttga
    13561 ttttcaaata attattctca ataaaacgat cactgaatat ccaaggaaaa tctgctagaa
    13621 tagatacttc aaattgccca tactctggta cttgcatcaa atattcttca atgatacgac
    13681 attcataacg caaaaattca aattccgtct cagatacttc tgtttttttg gacatgtgca
    13741 gattcaaaac caaatagtac agatcttctg ttttcttata gtgcctgcgg ttattagcaa
    13801 tttctttgta aggatcaaag ctgtcttctt gaatccgatc tagcattcgt ctttttttct
    13861 ttaatttttt tggctcatca agctgatttc gtatccattc atattcggca agtgtcatgt
    13921 tcattcgctc taaaaatgat ttcaatgttc caaacgatac ttttttcact gaatttgtct
    13981 cgatcccgac tagcgaagtt ttagaacaga ttccttccgc taattgggtc tgtgtcagct
    14041 ttttgttgac tcgaagcttc ttaatcaatt ctccatcata cataatagtt ctcctttttt
    14101 aattgaaatt aacccttggc ttttccagaa tgcttgccaa taagaggaat gattatttca
    14161 atttaaattt tccgcattca ataaatagat acgttaaaaa taaaaaatac acgaaaaaaa
    14221 taaaaaaatg tttttctcat aaaaaatacc aaccttcaag catgagactg ctgtctaatc
    14281 cctgaaggtt ggtttgattg tttattgatc atttttttac tgctccgcat tggttcctgt
    14341 tactggatat aggatagtcg ttgttttatt gccgtttttt gaaaagacac tggtcgaatg
    14401 ttcacctttt tgcttttcga ttgcttcatt acgttccgct tcgtttttat agtcatattt
    14461 tttcggatca atcgtctcaa atccgtttgg cacataaaac cgcaagagat tttcctgatt
    14521 cagcttatct gaaagactta acgcagtctt gactttttcc tgatctttgg agatttcctc
    14581 gtcgattcct gctgctttcg tatcgatgat ttctcctgtc tgattattgt aagcttttcc
    14641 tcctaggacg gtgtatttgg gcgtcacgaa gttcccattt cgaaaagcaa ctacttgatc
    14701 gtgctgagaa gataataagt ctgtaccaaa atgaagataa tctttatcat cgattcccaa
    14761 tagatgtagt aatgtcggca aaacatcgat ttctccacca tattcatgat tgactgtccc
    14821 tttagtgtaa ccaggcatat ggatcatcaa tggcactcgt tgcatctgtg cgttgtcaaa
    14881 ttcatcccat gtatctggat ctttaccaag agcacttgct aaatctttat tatctgtatt
    14941 cgagatccca aaatgatccc cgtaaatcac aaagattgaa ttttggtaaa taccagattt
    15001 tttcaaatac gtaaaaaatt gttccaatga ttgatccaga taatgggccg ttcgaacata
    15061 attatctact gtgctattgc cagtattcaa agatggaaag tcgaaattct tgtcatccgt
    15121 ataataaggc gtgtggttgg ttacggaaag aaatttcgtg taaaacggct gttgtagatg
    15181 ttcaagatat tgggcagatt ctctgaataa atctttatct aatatcccat aacctaacgt
    15241 ttcatcggat tcatcaaaat aagatcgatc aaagaaattg tcatacccta aatttttata
    15301 tacatgatct ctattccaga aactagctac attcccatgg aaaacagcac ttgtatagcc
    15361 ttctgtttga ccaagggtcg ctggtgctgc ttgaaagaca ttatctgacc caagttctgt
    15421 gaataatgag ccttgaggta aaccaaatgt gccagtttcc aacatgtttt cagcatcact
    15481 tgtctttccc tgtcctactt catgaaagaa attgtcaaaa ctgatcgttg cttgattctg
    15541 gaaaatacta tttaaaaacg gcgtgacttc ttgtccgtct accttcatat tgatcaaaaa
    15601 ctgttgaaaa ctttctaaat gaaggacaat cacatttttt cctttagcaa tcccaaatgt
    15661 ttcaggattt ggctctgcat aatgtttctt tgtatagtct aaaacttcat caattccatt
    15721 actgcttgca tgcgcgcgaa cactgctagt cattcctgtt ttgattccgt cataaatagt
    15781 gtacgcatcg attcctagat atttgacaat ataggaacgg tcaaatgtcc gctgtagcaa
    15841 ttggggacgg ttggcttcgc tcaacgctaa atttcctgaa aaaatcaatc ctgaaagaca
    15901 ggtaacagcg atagccatcg gtttgctgac agggttgctt ttgataggta ttttcttgac
    15961 cttcaaccaa accagtagcc caatgaacac agcgatatcc aaccaataga tgacatcatg
    16021 gggtttcatc aaagaaaagg agcttcctga caacccttgt gacactttcg aaaagcctaa
    16081 tacagatttg actgtaatga aatctgtgaa ttcccgataa aaaataatat tcaagtacaa
    16141 caaaagcgta tttaaaatat caatgatcaa aagaatggct aacgctggct ttggcttttt
    16201 aatatacaaa gaaaggccaa aaaacaataa ggtagtcgca atcgggttga tccacaagat
    16261 aaagtactgg atcgtacctt ccacacctaa tgaaaaatct gcatagtagg caatgatcgt
    16321 cttcagccag aaaaagaaaa cagctgtcaa aacgattgct aaacgatttt ggcacaattt
    16381 tttaagttgt gtcacgtata tcacttctct ctattcacat tttttcttat tttgctcctg
    16441 aaaaatgaaa atttcctaaa aaagttgtta tcttaagatt tttttattct ttctaaagat
    16501 tattccattc atttttcaca gcataaaatc gtttcggtgt aagcggttaa aatagtaagg
    16561 agataaaaat cttgtggtga ccgatttact ctatagtatt caaaagtagt ttctcggtat
    16621 tcgtacaaaa aaaacatact aagattagag gggaacatca ttgacgaatg atgttctcct
    16681 ctattttcta tttttagatt atagtgaaga gtgaaagaaa gttcgaacaa agccttgagg
    16741 actacgagtc cctatcaaaa accgaacttc tttactgtca ttttagaatc aggtttacgt
    16801 atgttggggc catgagcccg tgtataggaa attggaatcg aaatgaagca gtaaagttct
    16861 ttcaacactt aaaaaggagg aaaacaaatg ccttacattc ttgcattaga tgtttcaatg
    16921 ggacacagct acagtgtcct atacaaagac gacacctgct tatttgaaga cgagatcgaa
    16981 cacactcaaa gaggatttca tgcattactc gaagagatcc agcacttgcc tgaacgtcca
    17041 ttgattgttt ttgaagcaac gggtatttat tccagaccag tcgaaaagtt ctttcaagat
    17101 catcactttt cttactgttt gttaaacccg ttagaagcga aaaaacagat ggaagaaaac
    17161 accttacgca gttggaaaac agataaatcg gatgcccatc ggctcgcaca gactcatctt
    17221 aaaaatcaac gtcaaccaaa agaagtacaa agcaattttt acttagagat gcgcgattta
    17281 gcccgttttt atcaagaaat tgagaagaaa atcactcgac tgcgcatgga tttacacaac
    17341 tgccttcagt tgacttttcc agagcttgaa cagttctttt caaacaggct gaccccttac
    17401 gcattgacat tgatccgtct ttttccccat ccagattttg tattggcatc tactcggaca
    17461 aaaataaaaa ataaactgat caacgaaaca cgggaaaaaa tctccgcgaa tcgtgccgag
    17521 caaaaggcag atcaaatcat ccactatgca cagtgtgcct atccagctgt tgaaaaagac
    17581 agtattcact gtcaaaaaac gatctattac gccgaacttc tccaagattt actcgaacaa
    17641 aaaaaagcac tggcgacaca aatgatcaaa aaagccgaag cctctccttg ctttttgttg
    17701 taccaaacgt tccctgggat tggcgcactc acagccgctt tattgctcgg tgaattagga
    17761 gatatcacac gatttaaaac ccataaacaa ttgaatgcct ttgtcggaat cgacatccga
    17821 cggtatcatt caggtaagta tactggccaa gaccgcatca ataagcgtgg taatcccaaa
    17881 gcacggaaaa ttattttctt caccattcga aatatgattc gccagcaacg cgcagctcct
    17941 aatcatattg tcgattatta ctacaaatta aaaaagcaac ctatccctaa gaaagataag
    18001 gttgccacag ttgcttgtat gaacaagctt ctgaaatgta tgcacgctat ggttagggca
    18061 catacagagt acgactatgc gtacgcggtc tcagtggacc attaactaaa ccacttacag
    18121 tataccactt ctttgcccag atcgaaaatt ttttacgaac ggggctttat ttagtgtgcc
    18181 ttgattttgt cgctttcgtt tacgagctat ttttcttgca ccttttactt gactaatcgt
    18241 aggaaagagg ctgtgacaaa attcttgtcg cagctcccta tttcttgatg caggacagct
    18301 ttttcacaac ctctccacta aacggtgttc aatcagctga ccttcggtat tagctcaaaa
    18361 aacggcaaaa tatgaggagc tattttcccg tttttttctc taatactcag gtcaaaccgc
    18421 tgatttcaca acctctgatt cacggtgttc cagaagtagc ttcttcggaa ataagccgaa
    18481 attttacaaa aacttgagaa gcacttttcg taaatttctt cttatttctt gaagctgacc
    18541 acttctgtca caacttctgt ttttcattgt ccataggttt gatattgtcc ttctgatgac
    18601 tcattcgtaa tgattgaggc ttgattttct tcatttcccg ttatcgtttg tccactgcca
    18661 gaatctgaag ggcttagttc tgtcgactcg tttttttgag aagaagcagt tgttttcttg
    18721 acttgttcgt tggtcttttt ccattccgct agtcgatcgg ccaccacttt tttgtccact
    18781 ttttcagaat aagctgtaac cacttttcca tttttcaaac gtgctacctt gtcgccatca
    18841 tgagtcaaaa gcaaaacgac caaatgtttg tctttgaatt gatattgtct gagagaaatc
    18901 gcactaagta cgtcatcgat attgataaat tgattttctt cttttccttt tggcaaatag
    18961 atgtccaatt cacctttttc gtaattggca gttaatttat attcctccca ccaataagtt
    19021 gagaattctt ttttgaaatt atctactgat tctttgcttg ctacgatttt ttctgtttct
    19081 ttctgagcaa gatctgctgt catttttcct gaaaatgctg cggataaaat aagcagtaag
    19141 aaaaaaccga ataaccaaaa gagaagaaac ggtctttttt tcctcatgcc ttcctccact
    19201 cctatttaat tttgactatt ctattatata caaaaaatga aatagaaaga caaaaaagtt
    19261 gaaaaagatc gtaaataatt gaacttcttt ttcctgaagc ggtaagaacg ggaattttca
    19321 tacattctct cttggctatt ctttaagctt caaatccatt tctgtctgat acatctttga
    19381 accaatgacc cgatttcttg atcgttcttt ttccatcttg tgctaaatct actgaaataa
    19441 aaccgtaacg attcttatag gcattggtcc acgaccagtt atccatgcat gtccacatat
    19501 gataacctaa acaattggca ccttcttgaa tggcttgatg gacatatttc agatgagatt
    19561 gaacaaattc gatccgataa tcatcgtgga tcataccatc ttccttgata aatcgtgctt
    19621 cattttctac acccatgccg ttttcagaaa taaaacagtc gatattccca taatgatctc
    19681 gtacattgat caaaatatca tagattcctt tttcatagat ctcccatccg cgataaggat
    19741 tcattttacg accaggcatt tcataattgt cgaagtaatc ctcaggcata ggaccgtttt
    19801 tagtatcgat tggtgtttct tttgctttta ttcggcgtgg ttggtaataa ttaattccca
    19861 aaagatctac tgtgttttct ttaatgatcg tcaaatcatc tggatgaatc tccggcatta
    19921 gatcgagttc ttttattctc tcaatcaatt cactgggaaa ctcgcctttt acagctggat
    19981 caaggaatga acgattgaag aacgcatctg ctattcgtgc tgctttaaca tcttcgggat
    20041 ttgattcgtc tcttggataa ctcggtgtca aatttaagat gattccgatc acaccgcttt
    20101 gatccatttc atggtaagct ttgatggcta atgctgaagc caacgcttca tgataaccaa
    20161 cttggattgc ctttttcata tctgattctt ccggataatg ccatcgatac aaatagcctg
    20221 cctcgactgg aacgattggt tcattatggg taaaccattt ttttactttc gagccaaaca
    20281 attgaaaaca actttttgcg tatttttcat aggcttcaac cgtttctcgg ttcaaccatc
    20341 cgcctttttc ttgcagatac atcggcatat cgaaatgata cagattaaca aagggttcga
    20401 taccgttttc aatcaactca tcaaaatagt gatgatagaa acgaacggct tcttcgttga
    20461 ttgttccaga ttcatcaggt atcaaacggc tccattggat agaggttcga aatgaattat
    20521 gcccagtctg tttcatcaac tgtacatcct cttgatactt cgtataaact tgcgaggttt
    20581 tttcaggtcc tacttgatca aagaactttt gcggttcgtt tttgtaccaa taatcaaaaa
    20641 tattctctcc ttttccatcg cctgaaattc ttccttctgt ttgtggacca cttgctgcag
    20701 agccccacca aaaatttttt ggaaattgat atcttttcat caaagcacct ctttttcttt
    20761 tttagttgat tccatgtatg atattttcca tcacaatcac tttttcattt tcttcctctt
    20821 cttttttgat tcgtttcaac agctgttctg ctgctcgttc cccgatttta tctgttggtg
    20881 gcgtgatcgt gatttttgga aggatactca gatcgttttt atctaaataa tcatagtgac
    20941 tgaacgcaat atccttcccg attctgacac cttcttgatt gaaacattcg atggcaccaa
    21001 tcgacatgtt gtaattgctg acaaaaatag cagttgcccc tttttctaat aatgcttttg
    21061 ccccgtaata gccgcttttt ctagaatagt ctcctgtaaa gatctctaaa tggatagacg
    21121 gatccttttg taccgcttct ttggctccag ctaaacgttc tcttgctacg taatctgttt
    21181 gagcggcagc aataaatcct attgttttat gtccttgtcc gccatgatga gcaatcactt
    21241 ttgctacact ttctcgatca ttcactacta ttgtatcaat acctgctggc ccacccggac
    21301 aattcaatgc gacaactggg atactcattt ttgtcagcat ttcaaatcca gaccactctt
    21361 cttctgataa gaagacgatc aatccatcga tcgcatgctt catcaggtat tcgatttttt
    21421 gttcgatttt ttctggctga tcggcaaacc cgcttaataa taagctatac ccattttctt
    21481 cagctactct ttcgatccct gaaacaacct ccgaaccaaa tcggttagat atcccgttta
    21541 tcaacagtcc aatagagaaa cttcgattat tcttcaatcc tttagcaatg atgttttctt
    21601 tatagtccaa cgctgaaatc gcttctgata ttttttccat attcttttcc ttcagctgct
    21661 gaccgtttaa atagcgggaa actgttccca ctgaaacacc agccatctta gccacgtcac
    21721 ggatcgatgc catacatttt cactcctgcc agttacattt gatttctttt tcttgttgtt
    21781 cgaccatgat gaccgctttc tttgcataat gccgcttgtc atcaatatcc atataactaa
    21841 agtcttcaga agtgagttca aaaacaagca gtctttcctc ttgagaccgc aattcatctt
    21901 tacgaaaagc tttcaataat ttcgttcttt ggatgacaac gccgtcaatt aatcggacat
    21961 agacaagagt acttgtttta cctgaaacac ttcctgtatt tttaacttta attgatatta
    22021 ttactttgtc aatatccgtt ttgacttcca tctgactgta ttcaaatgtt gtatagctta
    22081 gaccatgtcc aaaaggataa aatggacttc cgcttaaatc ataataattc tcttgtttag
    22141 aagcagcacg ctgatagtaa taaacaggta gttgccgact atttctagga taagacaaac
    22201 tcaatcgtcc gcttgggtta tttcttccag aaatcatctg cgcaatggct tttccaccct
    22261 cctgtccagg aaaccatgct gttgcaatgg cattcgtaaa tgactcgatt ttcatcaaat
    22321 cgtagggtct tccttgcaca agtatcgtga tgattggctt gcctagttga tacaattgtt
    22381 caatcagctg ttcttggtat ccacctaaag aaagagaagc aacatctaca ttttctccag
    22441 aatccatgtt gatccctttc gatgaaactg ccccgttacg aagaaattcc atatcaaagt
    22501 tccgcgcact tgatccacct agaactgcaa tgatcacatc tgagttttga gcaagggtca
    22561 ccgcttctaa taatttttct tcctgatcct cctgatttct tacttcacat ccttgtgcat
    22621 aagaaacctt tgattgggga aactctgttt ttatctcttg tactatcttt tttttcaaac
    22681 ggtcttctgt ttgtggtgca gagtagtctc ccaacaagtg gtaaacatcg tgagcatttg
    22741 gacctataac agcaatgttt tttacttgtt gatccaacgg caatataccg tcatttttta
    22801 gtaaagtgat actttctttt gctgtctcta aatttaagtg ctctgattcc tccatgagat
    22861 gatcccaata ctcctttgga tcgtttgcaa atggctgttc aaataaacct aataaaaatt
    22921 tgacagataa aactcggcga actgcttgat ctagtagctt ttcatctacg acttgattat
    22981 caatggcttc tgcaatcttg gtataggtct catcccacaa actgagatcg attcctgccg
    23041 ctaatgcata tgctgcagca gtctttttat ctgtaaaaac atcggataaa cgatccaaag
    23101 ccacaccgtc tgccataacg attccttgat aaccgatctc ttttcttaat gtttgctcaa
    23161 aaagcgctcg attgatatga caaggtatcc cgtcgatgtc attataagca gccatgacac
    23221 ctactgcatt tcttgtactc ttcaataatg gtccgtaaat atcggagaac tctctgcgtc
    23281 caatcgttac tgtgcctgaa ttatgaccgc ctaaagcttc tccttgtgcg atacagtgct
    23341 ttaacacaac accgatctga tccttcttct tattgatttc ctctactgtc tgatcaagaa
    23401 aagaaacttt ttctgaaata atctctcctt ggaaccctcg aacgaccgct tcagaaaaac
    23461 gagctgagag aatcggatct tccccaaagc actcttctgt ccttccccaa cgaggatctt
    23521 ttgccagatc caaagtcgac accagcgcta aatgaacacc tttagcagcc agttcttttg
    23581 ccatcaatcg actcgattgt tctaataatg ctacattgaa agtatttcca cggccgatat
    23641 tcgttggata agaaatccca tccaagcctt gatgaccatg tggacattct tctacaagca
    23701 aaactggaat tttccatcgc gaatgctgta gtacatattc ttggactagg ttagccacct
    23761 tccagctttc ttttgcagga atcccattct tataatctgt tttagaccac ggatcagagc
    23821 gaaacagacc gtataaggca cccagaccgc ctccccattt aacatgttct tttaactttt
    23881 ctgttaattc gatcttgtct ccgttttttt cataacattc ccaaccatac aaatgctggt
    23941 ttacctgtcc tactttttca gcaatattca tcctcgaaag taaatcttct actctttggc
    24001 ggatggttaa ctttgggttt tgatacgggt aatcgtacac cttaatatcc tccttctgcc
    24061 aattttgcct tattagcggc taatacaaat ggcaggaata aaaatacatc tagaatgata
    24121 agtattactg ttaagactgc tgctcgtata tcgcctcctg tagcaataaa agattgtata
    24181 accggtggag tgatccaagg tgcagcagca actgttttac tgatgattcc aagacttgtg
    24241 gctgcgtagg caataaccaa gttaatagat ggtataatag cgcatggaat catatatatt
    24301 ggattcatga ctactggtaa tccaaacata attggttccg taatattaaa taaacaagta
    24361 gtcagaccaa atttagcgat tgatcgctgt tcttgacgtt tagaagccca caagatagca
    24421 ataactaacg gtaaaataca cccgccacct ccgatcaatc caaatgcatt ggtaaagggc
    24481 tccgtaataa tattaggaat ttcttttcct actgaaaaag catccatgtt ttcctgaata
    24541 gactgtaaca aaggtgcttg ccgtatagga gccaaaatac tcgaaccatg cataccaaac
    24601 acccataaga aatcagagaa aaactgtaaa aataagatgc ctgggacgga taaaacaaaa
    24661 gctttcaatg gtgtctgaat cgctaattca attaattgcg gaacattcaa accagtcgta
    24721 tggtttaaag taaaaacgac aactgaaaaa aataaaatga caactatttc tggtactaaa
    24781 gaattaaatg acgcagctac tgccggtgga actccttctg gcatcctgat tttaaaactc
    24841 ttcattttcg ctaatttgat aaataattct gtactgacaa gggcagcaat catcgccgtt
    24901 gctaagccag acgaagaagt cagactttgt aagaaaaccc ctgtagcttc tgctgtattt
    24961 ccgtccatca aagtgactgt acttgataaa gacatcaaaa taaacattac ggctgtactt
    25021 agaattcctg catgaacagg actaaatgat ggatcatcaa tcacgcctgt ttggatatag
    25081 tgggttgcca aattccagcc gatattgtaa cacacgataa tagctaaaat actcatcgta
    25141 ccattattca cgattgaagc aagttcagcc aatggactta gatcaagtat tctttggact
    25201 gttgcatttg aaaaaatcag agcattcagc aaaaccatga cagaagcagc cattactaac
    25261 ggcatagtaa cgataaaact gtctcgaatg gccagcaagt gtcgctggct actcatctta
    25321 gatacgattg gtagcatttt ttcagatagt ctgtctacga ctttcattat ttcatctcct
    25381 agattctaaa attgaaccgg ttcattttta atatagcact ttttttaaag attgcaacac
    25441 atttttttaa aacgctttct aaaggtggtg gattttttat gacttttgaa aaattattct
    25501 atttaatggt attttgtgaa ttttttctct tccatccgtt tgaagtttta ctttccaatg
    25561 gtataatttc tttatcctat caaatggggg aagtattttg aagaaattaa tcgtactatt
    25621 tattttgtct gcgggacttt tgacattgac tggctgtgat ttgtttggcg gctctgacca
    25681 atcctcggac gaaacaaccg attcttctgt cttaaaagcg gaaaaagcag atatcaccaa
    25741 cagtgtatat acgctaaaag tacgtaaaga tatggattta tccgcgggac agttgacatt
    25801 tacagataaa aaaatggaat ggacacgcac gtatcagaca caaggagctg attcttctga
    25861 atcatctgaa gaaaaaacag tgctgacaga tatcaagatt actaccaaag atgacgaata
    25921 tattatttca ggaaaagaaa ataaaaaaga agcaacagtc acatttacca aaatcggtaa
    25981 ttaccgtttg aaggataagg atggcaacat ctacagtcta tagtgccatc tgttggatct
    26041 tcttttgttt ctccccttct tttctgctat agtaaagaag caagatgact gtaaaggaga
    26101 cgaaatggat gatcgatagt cctgattatt gtacatgtaa taacaacaaa aatatccata
    26161 cagaatttaa tgatcaagga agtttgagta tatgcgacaa ttgccacaaa ctagtcaaag
    26221 attcttttaa atcgtttgaa tcttatcctg tcaaaaatta tgtaaaaagc aaaagaagat
    26281 aagcaaaatc aagcaaaact cactatggct gatttttatg aacttcaaac tcagcttctt
    26341 cctttgatca agcttcaaaa actggaatca gtaaaacaaa cagtggatca tttcagttca
    26401 caaatcaaag agtaacgttt tatttatttc acatctttga atataaaata accaaacgat
    26461 agaaagactt attctacatc acgtagtaat ttttctttcc cgtcgtttgg ttttttactt
    26521 ttagcctaat tcttgacctt tcgcatgctg attatctgta aaatattttc cagcaggctt
    26581 tccttccaat tcagctaatt tttcttggat ctgacgaatc accttttttg catcttctgg
    26641 attttctaca aaatcgaatg tatccccatc cacttgtatt ttaggacata catcaaaatc
    26701 ctcataccat tgattatagc gggtgttcag tgtttcgtaa taggtgtata acgtttcatc
    26761 gctttctagc tgttcatagt cacgcccgcg ttttttgata cgttctagca tcgtgtccaa
    26821 agaaactttg atatggacca tcaaatcagg acgttttttt ggagcaacat catctaactc
    26881 gtttagcatc gtatctaata agctatcgta ctgctgtact tcgatatccg ttacccgacc
    26941 taaatcagca tttagatgga acaacagact atcttcgtag atggagcggt ctagcacatt
    27001 gtcatcgttc actaaagcat ttttcattgc taaaaatcgc ttattcaaaa agaagatttg
    27061 caataaaaat gcgtactgct caggatttga ataaaaaagc ggcaatactt cgttatcttc
    27121 gacattttca taaaatggtt tagaatcaat ctcttttgca agcatatccg tcaaactgct
    27181 cttacctgca ccaatcgttc ctgaaatcaa aatcactgac atacaaccct tcctcttcta
    27241 taagtactcg cctacgtaag tttcaacagg caatttttca aaatcatatt cacgtacagc
    27301 ttggtctgat tttaaatacg tgtgaagact ttgtgtaatc atcaagttcg taggtaccgt
    27361 ctcttcttgt acgatgatca tcggtttttt caacatagtt gccacaccta attcgtaagc
    27421 cgttcctgga tcgattgttt gatgctcaaa gtcaaggata gccaaaacaa attctgcatt
    27481 tgtcaactct tccatatctt tttcgtatac ttcctgcgcc catccagcgc tgaatagttc
    27541 ataattgctt tcttggtgat gacgtggaga gtagaaactt gtgattgttt tgttttcctc
    27601 caacgctttt tcgatacgac ttactcgatc gatttgctcc tctgaaaaaa atggtcctgc
    27661 taaatatatt tgactcatta ttttgcctcc ttcaataata actgattttt aatagatact
    27721 gctcgtaatt cattgaccag ttccaatgga ttgttggaaa agatcacttg atccaaccat
    27781 tttttatcca taaaatcctc ttttgcaaaa gtttctaata attgttgcaa gccgctgtaa
    27841 taaccgccaa tattcaatat tccgattggc ttatcaggtg tcaggccgat cgctatccaa
    27901 gaaagaagtt gcgaaaattc ctccaatgta cctagacctc cagggaaaac gaggacagca
    27961 tctccttttt caaataataa tacctttctt tcatcaattg tctctgtagg aataaatgta
    28021 gtaacatctt tcccaggcag ctcagtttca aaaaggccat ttggatagat acctgtgact
    28081 tttccgttat tttccagtac tgttttacta atcactccca taatcccgga gtatgaacca
    28141 ccgtaaacca gttcgatttc ttcttgtgcc atatattttc ctaacgtttg cgcaataaat
    28201 ttgtaacttt ctttattgcc aaaacgggaa ccgcaaaaaa cagtaagttt
//
LOCUS       DAZKLD010000018        27806 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.018, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000018 DAZKLD010000000
VERSION     DAZKLD010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27806)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27806)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27806
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.018"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(328..2220)
                     /locus_tag="ACG8KR_000693"
     CDS             complement(328..2220)
                     /locus_tag="ACG8KR_000693"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0200092.1"
                     /translation="MSKTNTRPSHGPAGGPGHGGPGRNLGVKGEKPKNFWGTVRRLFG
                     YMSKRMVAIIAVFVLAIAAVIFQIQTPKVLGKATTEIFKGVMAGAQQMQQGQQVTKFP
                     IDFDKIAQIIATVIVMYLISAVFNFLQQFIMTRVSQRTVYELRHDLEAKMNRVPISYY
                     DTHTNGDIMSRAINDMDNIASTLQQNLTQFVTSAVTLIGVIVMMLSISWQLTLVALLM
                     IPLSLIIVMIVAPKSQVFFADQQKSLGLLNNQVEETYGGHTIVKTFNHTEKDQEVFEK
                     ENQNLYAAGWKAQFISAIIMPLMNFVKNLGYVFVAVLGGVKVANGNMTLGDVQAFLQY
                     TTQFSQPITQLASLMNTIQSTVASAERVFEVLDEEEMSNEKSGLPEEKDTPYKVRFEN
                     VQFGYTPDNLLMTDFNLDVKPGEMVAIVGPTGAGKTTLINLLERFYDVSGGSIRYDGV
                     DTRDLSRDELRAQFSMVLQDTWLFTGSIYDNIKYGNDDATDEQIIEAAKAAHVDDFVR
                     KLPEGYNTVLNEDASNISQGQRQLITIARAFLANPDVLILDEATSSVDTRTEILIQKA
                     MNRLLENRTSFVVAHRLSTIRDADNIIVMNHGSIVETGNHDTLMEKDGFYADLYNSQF
                     SEEEAS"
     gene            complement(2217..3944)
                     /locus_tag="ACG8KR_000694"
     CDS             complement(2217..3944)
                     /locus_tag="ACG8KR_000694"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0200093.1"
                     /translation="MLKIVKRISLTSAIAAAVFMVIQVLADLYLPTLTSNIIDKGVAQ
                     GDVDYIWHTGFVMIGFSLISILAAIANTFFATRESQKLGKQLRTDVYKKSESLTKDAF
                     DKYGTASLITRTTNDVTQIQMVTQMFLRMMINAPITLIGASILAYQKDHQLTKIFLVV
                     IPVMIILIGGIMYFAVPLFKSMQKKTDRLNLVFREGLTGVRVIRAFDKTRFEENRFDL
                     ANKDYTNTAIKVNTIVALMMPMMTLIMSGTNVAITWFGGHYIADMTLEVGNLIAFMTY
                     AMQILISFMMLSAIFIMVPRAQASADRINEVLDEKIGIHDPENPKTVSFAGKNATLAF
                     NHVNYRYHGAEKLALEDIDFQAKSGEIVAIIGGTGSGKTTLVNLIPRLYDIESGSIQI
                     NGTDISDMTQYNLRELMGFVPQKAVLFSGTIRDNMQYGKPDATDEMIWKALEIAQAKD
                     FVSEMEDGLDSHVEQGGGNFSGGQRQRLAIARALVKTADIYVFDDSFSALDFKTDANL
                     RQALKTNMKESITVLVAQRVSTVMDADMILVLDEGKLVGKGTHEELLATNETYQEIVH
                     SQLREEDLA"
     gene            complement(3960..4610)
                     /locus_tag="ACG8KR_000695"
     CDS             complement(3960..4610)
                     /locus_tag="ACG8KR_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HFQ0200094.1"
                     /translation="MPTQTFFHLPKEKQKRLIEAARIEFSRVPLKEASIANIVKLADI
                     PRGSFYQYFEDKEDLYFYYFETMRRDSSKDMLQLMKESDGDLFKGFESYFSKMIYEIL
                     EGENASFYRNLFMNMDYRASRKVAPHTSRQKTPHDPHKEHKQDAKELLELIDRTKLKV
                     KNDHELELLIQLVMNTVFTTIAHSYRLFSTSEDYSVEQAISEFNLKLSWLKNGVYK"
     gene            4814..5110
                     /locus_tag="ACG8KR_000696"
     CDS             4814..5110
                     /locus_tag="ACG8KR_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFQ0200095.1"
                     /translation="MAQSISVQDFNKLPLDADSIVLDTRNTLDYTKNHLSHSLSIPAA
                     ILPRQLKNLDPKKLYYILSYTGRRSEALADQLTANGFRAVYIIGGMEALQSSVA"
     gene            5302..5922
                     /locus_tag="ACG8KR_000697"
     CDS             5302..5922
                     /locus_tag="ACG8KR_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HFQ0200096.1"
                     /translation="MASVLVVKGHPLTAEESRTVKALTSFLTSYKENHPDDEVTVLEL
                     YRDDIPEIDEELLSGWEALRAGAEFTSLSESQQQKIARFNELTDQFLAADKIVIANAL
                     WNLNIPTKLKAWFDTVNVAGKTFRYTENGPEGLVTGKKALHIQSNGGVYNGQDFASQY
                     VKGILNFVGIDQVDQLFIEGIDYDPDRADELMQNALDKAAALGKSF"
     gene            complement(6014..7180)
                     /locus_tag="ACG8KR_000698"
     CDS             complement(6014..7180)
                     /locus_tag="ACG8KR_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFQ0200097.1"
                     /translation="MEGKNKKNRPVSWFWRWFLNNQVVTALLVVLLILLIVFLFTKVS
                     YLFAPIWQFLAIVGLPIILAGILYYLMNPVVDYFEKRKVPRLYSIIGLFVLVVALIAW
                     GSVVIIPKIQEQTVSFISNFPQYVDTIDNKLTEILRDPLFNQFREQLETAGDKFMNSA
                     GDMIQDISKSTVQSLGSFVGAVATILVALLTMPFILFYLLKDGKQLAPYFVKFLPTRM
                     QKPTLNVLSEMNSQVSSYIRGQLTVAFAVAIMFMIGFAVIGLEYAVTLGIIAGFLNLI
                     PYLGSFLAMIPAIFLAIVAGPFMIIKVLIVFAVEQTIEGRLISPLVLGNQLAIHPVTI
                     LLVLLTSGKLFGIIGVILGIPVYAAAKVVITHVFEWYTTISSLYEENPLKKQKE"
     gene            complement(7192..7545)
                     /locus_tag="ACG8KR_000699"
     CDS             complement(7192..7545)
                     /locus_tag="ACG8KR_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HFQ0200098.1"
                     /translation="MIQAKITEIGTEAINKEEPMLILFDKTATAALRKYSVIQEVSKD
                     EPFSLKAGGTIAFDDQVYTIDYVGPMANGNLKTVAHVSLIFDACPEEGQIANGIYLHP
                     HRLPAIGLGSQIFYN"
     gene            7673..8695
                     /locus_tag="ACG8KR_000700"
     CDS             7673..8695
                     /locus_tag="ACG8KR_000700"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HFQ0200099.1"
                     /translation="MIEKIILGTYTRRESKGIYTIDLDTEKEELANLSLAVEENSPTY
                     VAKSKAGNLYTVTSVDGLGGAGAYDKDFHFLNAVTESGAPLCYVAVDEARQLLYGANY
                     HKGEVNVYHILGDGELKAADSIFHQEATGPHKNQDHAHVHYTDLTPDQRLVVCDLGTD
                     RVYTYDVSENGKLNLVTTFTAEPGTGPRHLVFHPNGQFAYLFGELDSTVRVLSYNKET
                     GAFEELQKISTLPEEFDGENGGAAIRISNDGKFLYASNRGHNSIAVFAVFEDGSHIQN
                     KQIISTEGDFPRDFALDPSNNFVVCANQNTDNLTLYRRHPETGLLELLKKDVYVPECV
                     CVYFDR"
     gene            8714..8875
                     /locus_tag="ACG8KR_000701"
     CDS             8714..8875
                     /locus_tag="ACG8KR_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0200100.1"
                     /translation="MNQQDVLDRLREELNIPFFDGKIEDKEYSEEEYQKLKADLQNYF
                     EQYVQNVEN"
     gene            complement(9134..9568)
                     /locus_tag="ACG8KR_000702"
     CDS             complement(9134..9568)
                     /locus_tag="ACG8KR_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0200101.1"
                     /translation="MLPNIGYYVQSIDQIVKETEAIGEGMHPKYEVIRNAIDEGKTSE
                     LDKSTLEEIILLFEEGTKKYRAMLMKVSTLRPPAKAMGIHKNFEKAYLSYVAGCEEMI
                     QSIDLESGVDEDLFNTSEAKQDQATDQLTAAIQKMTNLLLKK"
     gene            complement(9678..11537)
                     /locus_tag="ACG8KR_000703"
     CDS             complement(9678..11537)
                     /locus_tag="ACG8KR_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFQ0200102.1"
                     /translation="MKRYISRLSTKQKKILKGTAAIISIFFLVVFSNLFLQWCQNDLS
                     VDLALKFAFSWHTEKFFLACLVLLIILIFLIALAGSVPLGSLTYVVAIGVLGFANYMK
                     MSYRQEPIYPDDLKMITEIGLLKDMTGTMLFTVILAAAGTVIGLFCWYMFRSLKKGRR
                     FQLIRLTTLLVAIGLLGYISNFNNPDNLLRKAYNKTALWIPYSQKMNYYNTGFIGGFL
                     YNLKVEPMDEPEGYSKEKIKEITEKYQKLADEKNKTVEEESPNIVFVMSESFSDPSRL
                     NGVEVSGEPLADYYEVADQTYSGNMLSQNYGGGTANIEFEALTGFSMALFNAQLTTPY
                     TMLVPKMDQLPSIVSALNAQSYQTTAIHPYNTSMYKREDVYQTLGFDQFISERTMTYT
                     DTIENNPYISDESAYKEVMTLLKEEKTPQFIHLVTMQTHMPYDGKYDNLDYPAKTSDG
                     SGTLDLENYLQDISYSSTALKQFTEELKNLSRRTLVVFWGDHLPGIYSDTIQAKNDKQ
                     TLHETQFLMFDSKGELEKQTTHDAITSPFYFAANLMEQTNQTTNGFYQLLLSLEQELP
                     AFERELYYQNGQWYKEAQFNRSQQEIYDEYQLIQYDIVAGKQYSLADGFFEHE"
     gene            11538..11678
                     /locus_tag="ACG8KR_000704"
     CDS             11538..11678
                     /locus_tag="ACG8KR_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoglycerate dehydrogenase"
                     /protein_id="HFQ0200103.1"
                     /translation="MLAPSFLSIKECCFSYFSCTKFESKERKEELMKSKYSLSLWPFW
                     FL"
     gene            complement(11655..12814)
                     /locus_tag="ACG8KR_000705"
                     /pseudo
     CDS             complement(11655..12814)
                     /locus_tag="ACG8KR_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332175.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
     gene            complement(13121..14545)
                     /gene="araA"
                     /locus_tag="ACG8KR_000706"
     CDS             complement(13121..14545)
                     /gene="araA"
                     /locus_tag="ACG8KR_000706"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008733 - L-arabinose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HFQ0200104.1"
                     /translation="MLNIGEKEFWFVVGSQHLYGEEALREVKKHAQEMVDELNENGQL
                     PYPIRLQELAVTADTITKIMKEVNYREEVAGVITWMHTFSPAKMWIRGTKLLQKPLLH
                     LATQYNESIPWKTIDMDFMNLNQSAHGDREYGFINARLNKQNKIVVGYWKRPEIQKEI
                     ADWMDVAVAYNESFGIKVARFGDNMRNVGVTEGDKVEAQIQFGWTVDYFGIGDLVQVI
                     DRVSDEEVDQLFEEYKELYTFDYGDYEEKTWEEHVKVQAQQEIGIRRFLEEGGYNAFT
                     TNFEDLYGMKQLPGLAVQRLMAEGYGFAGEGDWKTAAIDRLLKIMARGKDTGFMEDYT
                     YELASGQEAILESHMMEVDPTLAATKPRIVVSPLSMGDREDPARLVFDGKAGEGVVVS
                     MADFGTHYKLLINEVEAFEPTTEAPNLPVARVLWKTKPNFHEGVHSWIQAGGGHHTVV
                     SLNLTTDQIETWAKLVELETVVIR"
     gene            complement(14561..15259)
                     /locus_tag="ACG8KR_000707"
     CDS             complement(14561..15259)
                     /locus_tag="ACG8KR_000707"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019569 - L-arabinose catabolic process to
                     xylulose 5-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFQ0200105.1"
                     /translation="MLEQLKEEVYQANLDLPKHGLVKYTWGNVSAFDPETRYFVIKPS
                     GVSYEEMTADDMVVVDLDNHIIEGKLNPSSDTPTHAVLYRSFPQIGGIVHTHSTWATI
                     WAQAGLDVPAMGTTHADTFYGSVPCARFLTQQEIDSGYEYETGKVIIETFKERKIDPL
                     AIPGVLLHGHGPFTWGKDAQSAVMNAVVLDEVCKMNLFTRQLNSFSEELPQRILDKHY
                     LRKHGKNAYYGQNK"
     gene            complement(15216..16877)
                     /locus_tag="ACG8KR_000708"
     CDS             complement(15216..16877)
                     /locus_tag="ACG8KR_000708"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - D-xylulokinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0005998 - xylulose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HFQ0200106.1"
                     /translation="MKEVNAQSMLDVIDGRTSLGIEFGSTRIKAVLIDSSFTPIAAGS
                     YEWENKLENGIWTYSLEDIWKGLRVSYRKMAAEVFEIYGENLTTIGSIGFSAMMHGYL
                     AFDSKEQLLVPFRTWRNTITEEAERILTEKFQYNIPQRWSIAHLYQSILNKEEHVKEI
                     NFLTTLAGYVHWQLTGEKVLGVGDASGMFPIDPSTKTYHQEMKNIFGQLIEKAHVAID
                     LDHILPKVLVAGEEAGKLSESGAYLIDPTGNLRPGIPVCPPEGDAGTGMVATNSVAKG
                     TGNISAGTSAFAMIVLEKELENVHPEIDMVTTPSGELVGMVHTNNCSSDINAWVSIFE
                     EFTNSLGMEISRDKLFTVLFNKALEGDTDCGGLLSYGYFSGENITGVNEGRPLFVRTP
                     GSRFDLANFMRIHLASAFGAMRIGMDILKSENVQIDRLVGHGGIFKTPEVGQRILASA
                     MEAPVTVMDTAGEGGAWGIALLAAYMMDRHGKSLENFLAEDVFGQDQGITITPSAEEI
                     AGYQIFMRRYREGIDIEKTAIHQLQEKGVIENAGTTERRSIPSKS"
     gene            17095..18495
                     /locus_tag="ACG8KR_000709"
     CDS             17095..18495
                     /locus_tag="ACG8KR_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051119 - sugar transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HFQ0200107.1"
                     /translation="MKKEKKVSSSFIYFFGAFGGILFGYDIGVMTGALPFLQHDWGLA
                     GKASLIGWITSSVMLGAILGGSLSGLLSDKLGRRKMILLSALIFMAGSVLSASAPHNG
                     SYFLIAARILLGLAVGAASALVPAYMSEMAPARLRGRLSGINQVMIASGMLLSYVADY
                     LLKGLPETMAWRVMLGLAAVPALILFFGVLALPESPRFLMQSGRLEEAKRVLNYIRTP
                     KEAEQEFEQIQLNVKQEKTTGTSWHTLFLEKYRSLVIAGIGVAVFQQFQGANAIFYYI
                     PLIVEKATGHAASDALMWPIIQGIILVAGSLLFLVIADKFNRRTLLKIGGSVMGLSFI
                     LPAVLGTVLDAHTNSLLILLFLCIYVAFYSCTWAPLTWVIVGEIFPLAVRGRASGLAS
                     SFNWIGSFLVGLLFPVMTASMSQEIVFGIFGIICFLGVLFIQEIVPETRGKSLEEIEQ
                     SASKKTYPKRISIHHS"
     gene            complement(18747..19826)
                     /locus_tag="ACG8KR_000710"
     CDS             complement(18747..19826)
                     /locus_tag="ACG8KR_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFQ0200108.1"
                     /translation="MAKYQDIAEEIKQKILREEYEVNTTIPSEFKLQEIYDVSRHTIR
                     QAIAVLVNEGYLRKEKGSGTYVDDSYKKEKTPLNRTKTIGVITTYLSDYIFPSIIRGI
                     ENELRESGYSLLLASTNNDHELEKECLKKMIAYGVDGLIVEPTKSNQYNPNLALYVAL
                     REQGIPMVMINAAYEELNASYICLDDVKVGFMAAEYLIKKGHQRLLFITKIDDLQGKY
                     RMKGFISACEQYNVRFLTEDIITFTTETRETIAEKTVQRLKEEQALDGIICYNDQIAS
                     LMLDKLTENGYHIPDDLSIVGTDDSSLSHLGNKKLTTLTHPKEKLGQDAARWIVTTIE
                     KGQAQPNILYEPVLVERETVKENEK"
     gene            complement(19912..20871)
                     /locus_tag="ACG8KR_000711"
     CDS             complement(19912..20871)
                     /locus_tag="ACG8KR_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HFQ0200109.1"
                     /translation="MMKTLYNNPIVRQRADPWVYKHTDGYYYFTGSVPGYQVIELRRA
                     KSLNELEHAASLIVWQAHEEGPMSELIWAPEVHYINGKWYIYFAASNHKTIRDESHHH
                     RMFVLENQHIDPMNTDWIEKGQIKTKRETFSLDGTTFSSQGKLYYVWAQQDPEITGNS
                     NLYLSEMDNPWTLKGKQILLSIPEYEWEKRGFSVNEGPAVLTRNGKIFITYSGSATDE
                     NYAMGLLWADEHKNLLLKESWHKLSEPVFVSSEKNKQFGPGHNSFTVSEDGCFDTLIY
                     HARPEKNQKGDPLDNPNRHANAQCFTWNEQGFPEFGEPVPYAL"
     gene            complement(20883..22394)
                     /locus_tag="ACG8KR_000712"
     CDS             complement(20883..22394)
                     /locus_tag="ACG8KR_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046556 - alpha-L-arabinofuranosidase
                     activity [Evidence IEA];
                     GO_process: GO:0046373 - L-arabinose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-arabinofuranosidase C-terminal
                     domain-containing protein"
                     /protein_id="HFQ0200110.1"
                     /translation="MKVKIREDFTVGEISSRLFGSFVEHMGSVVYTGIYEPGHETADG
                     DGFRQDVLSYVKELELGVIRYPGGNFTSGYDWVDTVGRKEQRPKKIDLAWRGIEPNSF
                     GLHEFFKWISHVQAKPIFTVNLGTKGIDEAKNLVEYCNFPKGTFWSDLRIKNGQKKPF
                     GVKLWCLGNELDGPWQVGAKSAYEYGRLANEASKVMKLVDDSIETILVGSSTPRLPSY
                     PEWDRIALEEAYENVDYLALHNYIDKYDEEDLTKPPKNERDDTATYLAKAYRFDRQIE
                     EIVATCDYVKGYLRSEKTMYLAFDEWNVHAQPEKRHRDFEVGSPIDWCYFTMEDTLLF
                     GSLGLAILRHADRVKISCQSLLVNTIPLILTDAKGQAWRNPTYYVMQHLSKYAKGQVL
                     KQQIICPVYDCEKIKNVPYVDSVIVDNGNQLTIFLINRTNEQQKITIEHDFNLLDQGE
                     HYTLTSSDLLDKNTREEPEKIKPEINYFSIEKHDELNVGIEKYSWNVIRIKKM"
     gene            complement(22416..22979)
                     /locus_tag="ACG8KR_000713"
     CDS             complement(22416..22979)
                     /locus_tag="ACG8KR_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HFQ0200111.1"
                     /translation="MKIVETKLYGQLIVVSDYILLGILWVIVCLPVITIVPATCAVFY
                     VMAQWKGEGSGNIIYFFFQGIKQHFGKNLFLSLLTIIIYFLVNQLLEEQTIVLSISGY
                     VVLLIYSMFLLSWIYVISENKDITFLQIFEQSAWKVFFDFLRNILCCVLLLGFVLLIF
                     LFPPFIFIFAGGVWKLVSIILTIGNER"
     gene            complement(22985..23848)
                     /locus_tag="ACG8KR_000714"
     CDS             complement(22985..23848)
                     /locus_tag="ACG8KR_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0200112.1"
                     /translation="MAPESSITVTVNSRKAVSGKKIIRTISMLVILLVIAFPFLWLII
                     SSFKHEKDIISFPPRIFADSYTLDNYIKVWTTIPLLDYIKNTVIFAGGTVITSVFFDS
                     LAGYAFARMRFKGKSVLFYFVLLTMMIPFQVFMIPLFIEVNLLGMLDTYAGLIIPRMT
                     TAFGIFMMRSFFITLPDSLEEAARIDGLSEFNIFLKIMLPLSKPTLLSLGIFTLMNSW
                     NDLLYPLILTSSSKMRTLPAGLALFTGQNISFYGPVMAGTVISMLPLLVVYIFAQKYF
                     VQGTAMSGMKE"
     gene            complement(23862..24758)
                     /locus_tag="ACG8KR_000715"
     CDS             complement(23862..24758)
                     /locus_tag="ACG8KR_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0200113.1"
                     /translation="MNGFTKYWNRPDRAAYLFLTPSFLVLLLFTVIPLLGTFAISFTD
                     LNIFFTSRNFVSFENFTRIFSDERAVNSLIHSLYFTVLETPTQIIVGLLAAVALSKNT
                     RFNRFCRSTFYIPVICSLTSIAIIFSMLLDPNVGAIPYLFSQVGLPIPQFFRDPTLAM
                     PTVAIMTVWKNFGVTMTILLTAIQGISPSLYESAQMDGATNRQQFFNITLPQIVPSLG
                     FCILTNLIGSMQVFDQVYVATAGGPQFKTETAVQYIYSRGFTAPYELGYASALSSVLF
                     IIVATIAISTNVYMSRKEKQMK"
     gene            complement(24933..26222)
                     /locus_tag="ACG8KR_000716"
     CDS             complement(24933..26222)
                     /locus_tag="ACG8KR_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFQ0200114.1"
                     /translation="MKEEQKMKGKKWLIGSLLVAATGILGACGGGSSAESDSDKEVTL
                     TFWNGFTASDGEILQEIVNEFNKTNDKNIKIEMDIMTWANLNEKLPTAISSKKAPDFI
                     ALNYPDFAQYVENGAVQPLDDFWEYDGVDKSDFKETAIELGEVEGKQYFVPMQVQGMY
                     MYWNNDLFKQAGLDTEQPPTTWEELTEMAPKLTDSSKNVSGFVFNKDGTAPLYNWMLA
                     NGGKLVNDDYTKSEFASPENLKTLKAIQKMIHVDKTGPESISGAEMDNLMNAGQLAIE
                     INGPWLNNGLKANEIDYNVTTLPQGSDSDKKTAILDGVGYAIPASTDNNKKEAVYEFL
                     KYWNTTEIGKKWSIENGFPAYLDSVAKDEEVKNDPIVSELSKQMDYAEPYLPGFTQIS
                     AINNDIINPLIEKLLAGEDPETLMNDADDAINQLLQK"
     gene            complement(26194..27711)
                     /locus_tag="ACG8KR_000717"
     CDS             complement(26194..27711)
                     /locus_tag="ACG8KR_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HFQ0200115.1"
                     /translation="MEATMLISSVKRVGKVDPRLYSSFIEHMGRAVYEGIYQPDHPSA
                     NKSGFRQDVIHLVKELNIPMIRYPGGNFVSGFRWEDSIGPIANRPRRLDLAWRTIETN
                     EVGIHEFYNWCQHVGAEINMAVNLGTRGVDAARNLVEYCNFPKGTYWSDLRRSNGQES
                     PFGIKTWCLGNEMDGPWQIGHKTAEEYGRLAEETAKAMKLVDDSIEVVLCGSSNRQMP
                     TFGDWELTVLDQAYDQIDYLSLHQYYGNQKNDLSHYLARSLDMDRFISEVTAMCDTVK
                     AKKHSKKQINLSFDEWNIWYHSAEQDEQAAPWQIAPPLLEDNYNFEDALLLGCLLITL
                     LKHADRVKIACLAQLVNVIAPIRTETNGEAWRQTIYYPFMQAAVYGQGEVLSPQIRSA
                     NYATEDFEEVPYLESIAVINDKELVIFAVNRGEEEMNLTVHLSELSLEGVIDFSEMSG
                     FDTKATNLPKSEQVKPSHSTNVKAEDGKINCLLKPLSWNVVRCRLSDEGGTKNER"
BASE COUNT         8116 a         5859 c         4819 g         9012 t
ORIGIN      
        1 ttgaacaccg tttaccaaga ggttgtgagc ctgccgttta actccgagtc acaaggagca
       61 atttcaaaaa acagcttctc aggtttgttg actattgtga cttgtgaccg aggagttggc
      121 aggagaacac cgtttaagga agaggttgta aaatcagcgg tttgacctga gtattagaga
      181 tacaaaacgg aaaaatagct tctcatattt tgccgttttt tgagctaata ccgaaggaca
      241 gctgattgaa caccgtttat tcggaaaaag ggactgtgac acgacttttg tcacagcccc
      301 taaaaaaaat aataaatttg tactattcta tgaagcttct tcttcagaaa actgactgtt
      361 ataaagatct gcatagaatc cgtctttttc catcaatgta tcgtggtttc ctgtttctac
      421 gatcgaacca tgattcatca caataatgtt gtctgcatcg cgtattgtag acaagcggtg
      481 agcaacaacg aaacttgtcc ggttttccaa taggcggttc atcgcttttt gaatcagtat
      541 ttctgttcgt gtatctacac tagatgttgc ttcatcaagg atcaggacat ctggatttgc
      601 taagaatgcc cgcgcaattg tgattagctg acgctgccct tgcgaaatat tgcttgcatc
      661 ctcgtttaat acagtgttat atccttctgg caatttacga acaaaatcgt ctacgtgagc
      721 agctttagca gcctcaatga tttgttcgtc tgttgcatcg tcgttcccat atttgatatt
      781 gtcatagata cttcctgtaa acagccaagt atcttgcagt accattgaaa actgtgcgcg
      841 caattcatca cgtgaaagat cacgggtatc tacaccatcg taacgaatac tacctccaga
      901 aacatcgtag aaccgttcaa gaaggttgat cagtgtcgtc ttccctgcac cagtcggacc
      961 tacaatagcg accatctcac caggtttgac atccaagtta aaatcagtca ttagcaagtt
     1021 atctggtgtg tagccgaatt gaacattttc aaaacgaact ttgtatggtg tatctttttc
     1081 ttctggaagg ccagattttt cattggacat ctcttcttca tccaacactt caaatacccg
     1141 ttcggctgaa gcaactgttg attggatagt gttcatcaaa cttgcaagtt gagtgattgg
     1201 ttgagagaac tgtgttgtat attgcaagaa cgcttgaaca tcccctaatg tcatgttccc
     1261 atttgcaact tttactccgc ctagtacagc gacgaacaca tagcctagat ttttaacgaa
     1321 attcatcaac ggcatgatga ttgccgaaat aaattgagct ttccaaccag cggcatacag
     1381 attctgattt tctttttcaa atacttcctg gtctttttca gtatgattga atgttttaac
     1441 aatggtatga ccaccatatg tttcttctac ttggttattc aataaaccta aacttttttg
     1501 ctgatctgcg aaaaacactt gtgatttcgg cgctacgatc atcacgatga tcaagcttaa
     1561 agggatcatc aataacgcga ctaacgtcaa ttgccagctg attgaaagca tcatcacgat
     1621 gaccccaatc aatgtgactg cactagttac aaattgagtc aaattttgct gcagcgtgct
     1681 tgcgatgtta tccatatcat tgattgctcg agacatgata tccccatttg tatgtgtatc
     1741 ataataggaa atcggtacac gattcatttt ggcttccaaa tcatgacgta attcataaac
     1801 tgttcgttgg gaaacacgtg tcataataaa ttgctgtaaa aagttgaata cagcagaaat
     1861 caaatacatc acgattactg tcgcaatgat ctgcgcgatt ttatcaaagt ctatcggaaa
     1921 ttttgttact tgttggcctt gttgcatctg ttgagcgcca gccattacac ctttgaatat
     1981 ttctgtggtt gctttcccca agacctttgg tgtttggatt tggaagatca cggctgctat
     2041 tgctagtaca aatacagcga tgatcgcaac catacgttta gacatatagc caaacaatcg
     2101 gcgtaccgtt ccccagaaat ttttaggctt ttcacctttt acaccgagat ttctgccagg
     2161 tccaccatgt cctggaccgc ctgctggtcc atgagaggga cgtgtgtttg ttttactcat
     2221 gctaagtcct cctctcttag ttgtgaatga acgatttctt gataagtttc gtttgttgct
     2281 aatagttctt catgtgttcc tttgccaacc agttttcctt catctagcac aaggatcata
     2341 tctgcatcca ttactgtact tactcgttga gcaactaaaa cggtgatact ttctttcata
     2401 ttcgttttca atgcttggcg aagattggca tccgttttga aatccaacgc tgagaaggaa
     2461 tcatcaaaca catagatatc tgctgttttg actaatgccc gtgcaattgc cagacgctgt
     2521 ctttgtccac cagagaagtt tcctcctcct tgttccacat ggctatctaa gccatcttcc
     2581 atctctgaaa caaaatcttt agcttgtgcg atttctaatg ctttccaaat catttcatct
     2641 gttgcatccg gcttaccata ctgcatattg tctcggatcg ttccagagaa cagaactgct
     2701 ttttgaggaa caaagcccat taattctcgc aaattatatt gagtcatgtc actaatgtcc
     2761 gttccattta tttggatcga accagattcg atgtcatata atcgagggat caaattgact
     2821 aaggtcgttt tacctgaacc agtcccccct atgattgcga cgatctcgcc cgacttggct
     2881 tggaaatcaa tatcttccaa tgcaagtttt tctgctccat gatatcgata attgacatga
     2941 ttgaaggcca atgttgcgtt ttttccagca aaagaaactg ttttaggatt ttcagggtca
     3001 tggataccga ttttttcatc caacacttca ttaatacggt cagctgaagc ttgtgcacga
     3061 ggcaccatga taaaaatagc ggaaagcatc ataaagctga tcaagatctg catcgcataa
     3121 gtcataaatg caatcagatt accgacttcc aaagtcatat ctgctatgta gtgtccacca
     3181 aaccaagtga ttgctacgtt tgtaccgctc atgatcaatg tcatcattgg catcatcaat
     3241 gcgacgatcg tattaacttt gattgccgta tttgtataat ctttattcgc taaatcaaaa
     3301 cgattttctt caaagcgtgt cttatcgaat gctcgaatca ctctgacccc tgtcaatcct
     3361 tctcgaaata ccaggtttaa acggtcagtt ttcttttgca tacttttgaa taatggaaca
     3421 gcaaagtaca tgattccgcc gatcaaaata atcattactg ggatgacaac taagaagatt
     3481 tttgtcaact gatgatcttt ttgataagca agtatgctcg cgccaatcaa cgtgattggt
     3541 gcattgatca tcatacgtaa aaacatttgt gtaaccattt ggatttgagt cacatcattg
     3601 gtcgttcttg tgatcaaaga ggccgttcca tatttatcaa acgcatcttt tgtcagactc
     3661 tctgattttt tatagacatc cgtacgcaat tgcttaccca gtttctgtga ttcacgagta
     3721 gcaaaaaatg tatttgcgat agcagctaag atactgatca aagaaaagcc gatcatcaca
     3781 aaacctgtat gccaaatata atcgacatct ccttgcgcta cccctttatc aataatattg
     3841 gaggtaagtg tcggcaaata taaatctgcc agtacttgga tcaccatgaa aactgcagct
     3901 gcaattgctg aagtcaagga gatccgtttt acgattttta gcatctagtt cccttctttc
     3961 tatttatata ctccgttttt cagccaagaa agtttcaaat tgaattcact gattgcttgc
     4021 tctactgagt aatcttcaga tgttgaaaat aagcgatacg aatgcgcaat tgttgtaaat
     4081 acagtattca tcactaattg gatcagtaat tccaattcat gatcattttt tactttcagc
     4141 ttcgtccgat cgatcaattc aagcaactct tttgcatctt gtttgtgttc tttatgtgga
     4201 tcgtgaggcg ttttttggcg actcgtatga ggagcgactt ttcttgaggc acggtaatcc
     4261 atgttcataa acaagttacg ataaaaagaa gcattctcgc cttccaaaat ctcataaatc
     4321 attttagaaa aataggattc gaatcctttg aatagatcac catcggattc tttcattagt
     4381 tgtaacatat ctttagagct gtcacgtctc attgtctcaa agtaataaaa ataaagatcc
     4441 tctttatctt caaaatactg atagaaactg cctctaggga tatccgctaa tttgacgata
     4501 ttagcaatcg aagcttcttt taagggtact ctagaaaact cgattctcgc agcctcgatt
     4561 agtctttttt gtttttcttt aggcaaatgg aaaaatgtct gtgtcggcat atggattctc
     4621 ctttctttca agatgacaca ttgtcatatg acgctatgtc attataacac ctcctacctg
     4681 ttttgcaaga catttgcata gtttttttca ttatcatccg atacattaaa aaagtgataa
     4741 gaggaatcct tccatttgaa ttaatgagag ttgcccatta aaatagccac atactaaaca
     4801 aaggatgaga taaatggccc aatcaattag tgtccaagac ttcaataaat tgcctctcga
     4861 tgctgacagc atcgttttag atacaagaaa taccttagat tacacaaaaa accacttaag
     4921 ccactcgctt tctatccccg ctgctatctt gccaagacaa ttaaaaaatc tagatccaaa
     4981 gaaactttat tatattctaa gctatacagg caggcgatca gaagcattag ccgatcaact
     5041 tactgctaat ggttttcgtg ctgtttatat cattggtggg atggaagctc ttcagtcgag
     5101 tgttgcttga atattgcttg ttagattcaa aagaggctgt gacaaaaaat cgtgtcacag
     5161 cctcttttat accctgtctt tttttagtta tttattcttc aaccttatca atatgacttg
     5221 atagcctttt ctttttcgag taaactgatt ttgttagtca tagttataac aatcatagca
     5281 ataaaaggag aaggtaagca tatggcatct gttttagtag tcaaaggtca tcccctaact
     5341 gcagaagaat cgcgcacagt taaggcattg acgtcttttt taacaagtta taaagaaaat
     5401 catccagatg atgaagtgac tgttttggaa ctttatcgcg atgatattcc ggaaatcgat
     5461 gaagaattat tatcaggttg ggaagcatta cgtgctggtg cagaattcac ttccttatca
     5521 gaaagtcaac aacaaaaaat tgctcgtttc aatgaattga ctgatcagtt ccttgctgct
     5581 gataaaatcg ttatcgcaaa cgccttatgg aatctaaata tcccaacaaa attaaaagct
     5641 tggttcgata ccgtcaatgt agctggaaaa acgttccgct atacagaaaa cgggccagaa
     5701 ggattagtga caggtaaaaa agcactgcac atccaatcta atggcggtgt atacaacgga
     5761 caagactttg cctctcaata tgtaaaaggt attttaaact ttgtaggcat cgatcaagta
     5821 gatcagctat tcatcgaagg aatcgattat gaccctgacc gtgcagatga gttgatgcaa
     5881 aatgcattag ataaagctgc tgctttaggt aaatcatttt aaaaattgag ataaatgcat
     5941 acaaaagaga cagaggaaaa aatcttcaaa gcagcatttg tgccttggat attttcccat
     6001 ctgtctcttt ttgttattct ttctgctttt tcaacggatt ttcttcataa agagaagaga
     6061 ttgttgtgta ccattcaaaa acatgagtaa tcactacttt agctgctgca taaacaggaa
     6121 tccccaagat cacaccgatg attccgaaca atttcccaga agtcagcaga actaataaaa
     6181 tcgttactgg atggattgct aactgattcc ctagtactaa tggagagatc aagcgtcctt
     6241 ctattgtctg ctctactgca aaaacgatca gtactttgat gatcatgaat ggacctgcta
     6301 caatggctaa aaagatagcc ggaatcatcg ctaaaaatga tcctaagtaa gggatcaggt
     6361 ttaaaaaacc cgcaataatc cccaacgtga cagcatactc taaaccaatg accgcaaaac
     6421 caatcataaa catgattgcc acagcaaaag ccacagttag ttgtcctctt atataagaag
     6481 aaacttgact attcatttca ctcagcacat tcaacgtcgg tttttgcatg cgtgtcggta
     6541 aaaatttgac aaaataaggt gctaattgtt taccgtcttt caatagataa aataagataa
     6601 acggcatcgt cagcagagcc actaaaatag tcgctaccgc gccgacaaaa cttccaagac
     6661 tctgaactgt actcttagat atatcttgga tcatatcgcc tgccgaattc atgaacttat
     6721 cacctgctgt ttccaactgt tcgcgaaatt ggttgaacag cgggtcccgc aaaatctcgg
     6781 ttaatttatt atcaatcgta tctacatatt gagggaagtt actgatgaag ctcactgttt
     6841 gttcctgaat cttagggatg atcactacgc tcccccatgc gattagtgca acaaccaaaa
     6901 caaacagccc aatgatactg taaagccttg gtacttttcg cttttcaaag taatcaacta
     6961 ccggattcat caagtaatac aagattcctg ctaaaatgat aggcagacct acgattgcta
     7021 aaaattgcca gattggtgca aacaaatatg aaacttttgt aaataaaaag acgatcagca
     7081 atattaacaa tacaacaagt aaggctgtga ctacttgatt atttaaaaac cagcgccaaa
     7141 accacgatac cggtctgttc ttcttatttt ttccttccat aattcctcct gttagttata
     7201 aaaaatttgg cttcccaaac caatagcagg cagtctatgc ggatgaagat agattccatt
     7261 tgcaatttgt ccttcttctg gacaagcatc aaaaatcaat gatacgtgag ctactgtctt
     7321 taaattccca ttggccatcg gaccgacata atcgatcgta tatacctgat catcaaaagc
     7381 aattgttccg cctgctttca aagaaaaagg ttcgtctttt gatacttctt gaatcaccga
     7441 atattttcgt aaggcagctg ttgctgtttt atcgaacaaa atcagcatgg gttcttcctt
     7501 gtttattgct tctgtcccaa tttctgtaat ctttgcctga atcattgcac tctctccttt
     7561 aattgctttc aaaaacagta taacacattc tcattcttta gccgtgtgga ataattatgt
     7621 gatagaataa aaacgaaaac tatttcatta caattaggag gaatcagctc atatgatcga
     7681 aaagataatt ttaggaacct atacgcgtcg cgaaagtaaa ggaatctata cgatcgacct
     7741 tgatacagaa aaagaagaat tagccaacct ttcattagct gttgaagaaa atagccctac
     7801 ctatgtagca aaaagtaaag ctggcaatct ttacacagtt actagtgtgg atggattagg
     7861 tggtgccggt gcgtacgata aggattttca ttttttgaat gcggtaacag agtctggcgc
     7921 tcctttgtgt tatgtagcgg ttgatgaagc acgtcaatta ctatacgggg cgaactatca
     7981 taaaggtgaa gtaaatgtct accatatatt aggagatggc gaactgaaag cagcagattc
     8041 aatattccat caagaagcga caggtcctca taaaaaccaa gatcatgcgc atgtccacta
     8101 tacggatctg actcctgacc aacgattagt cgtatgtgat cttggtacag atcgtgtgta
     8161 tacttatgat gtgtccgaaa acggcaaatt gaatcttgta actaccttca ctgcagagcc
     8221 aggaacaggc cctcgccatc ttgtctttca tccaaatgga caattcgctt atttatttgg
     8281 tgaattagac agcacagtcc gcgtgctttc ttataataaa gaaacaggtg cttttgaaga
     8341 attacagaaa atatcgacct tgccagaaga attcgacggt gaaaatggag gggcagctat
     8401 ccgcatttca aatgatggca agttccttta tgcttctaac cgaggacata actcgattgc
     8461 tgtattcgca gtttttgaag atggttccca cattcaaaac aagcaaatca tctcaacaga
     8521 aggggatttt ccccgcgatt tcgcactaga tccaagcaat aattttgtcg tgtgtgccaa
     8581 tcagaacacc gataatctga cattatatcg ccgtcatcct gaaacaggat tactggaatt
     8641 actgaaaaaa gatgtctatg taccagaatg tgtctgtgtt tattttgatc gttagtaaga
     8701 aaaggagaat gaaatgaatc agcaagacgt attagacaga ctacgggaag aactaaatat
     8761 tcctttcttt gatgggaaaa tcgaagataa agagtatagc gaagaggaat atcagaaact
     8821 caaagccgat ttacagaact actttgaaca atatgtacag aatgtagaaa actaatcagc
     8881 tttccatact atctgaaaag aacatcagga caagactttt ccgtctatcc tgatgttctt
     8941 ttctacaaat aaaaaaccgc cgagatggtt agactcgacg gaaaaggagt atttactcga
     9001 ttttgagtaa gtacaaggtt gttgttggtt ataggtatgt cattatcata aaggattttg
     9061 atcactctat cataaaggta atattacatt tatattagat aactctagtt tttattgtta
     9121 tcaaagattt cttttatttt ttcaacaaca aattagtcat tttttggatg gcagctgtca
     9181 attggtctgt cgcttggtct tgttttgctt cagatgtatt gaatagatct tcatctaccc
     9241 ctgattcaag atcgatactt tggatcattt cttcacagcc tgctacatat gaaagataag
     9301 ctttttcaaa atttttatga atgcccattg cttttgctgg tggacgcaaa gtactgactt
     9361 tcataagcat tgcccgatat ttttttgttc cttcttcaaa taacaggatg atctcttcaa
     9421 gagtcgattt gtccagttca cttgtttttc cttcatcaat tgcgtttctg atcacttcat
     9481 atttcggatg cattccttct ccgattgctt ctgtttcctt gacgatttga tcgatggact
     9541 gcacataata gccaatattc ggtaacatgt tcgttttcct actttctcca ttgttgttat
     9601 tatttattct acttgttttt atcctcaaat gacaagtact tgaaaaaatt taatggtttc
     9661 tatacagctc gccattctta ttcgtgctca aagaaaccat ctgctaaact atattgtttc
     9721 cctgcgacaa tgtcatattg gatcaattga tattcgtcgt aaatctcttg ttgcgagcga
     9781 ttgaattgcg cttctttata ccactgtccg ttttgatagt acagttctcg ttcaaacgcc
     9841 ggaagttcct gttctaatga taaaagcagc tgataaaagc catttgttgt ctgattcgtc
     9901 tgttccatta gattcgctgc aaaataaaac ggactggtga ttgcatcgtg agtcgtttgc
     9961 ttttctaatt ctcctttact gtcgaacata agaaattgtg tttcatgcaa tgtttgtttg
    10021 tcattttttg cttggatcgt atctgaatag atccctggaa gatgatcccc ccaaaaaaca
    10081 actaaagtcc ttctagaaag attcttcaat tcttccgtga actgcttcag tgccgtgctg
    10141 ctataggaaa tatcttgcag ataattctct aagtcaagtg tcccacttcc atctgaagtt
    10201 tttgcaggat agtctagatt atcatacttt ccatcgtagg gcatatgtgt ctgcatcgtg
    10261 actaaatgaa tgaactgagg tgttttttct tccttgagaa gtgtcattac ttctttgtaa
    10321 gctgactcat cagaaatata tggattattc tctatcgtat ccgtataggt catcgtacgc
    10381 tcgctgatga actggtcaaa tcctaatgtc tgataaacat cctctctttt atacatcgaa
    10441 gtattgtatg gatggatcgc tgtcgtctgg tatgattgtg cgtttaacgc tgaaacgata
    10501 gaaggaagct gatccatttt tggtacaagc atcgtgtaag gggtggtgag ttgcgcatta
    10561 aataacgcca tagaaaaacc agtcaatgct tcaaattcga tatttgctgt tccaccgcca
    10621 tagttttgag aaagcatatt gccactatat gtttgatcag ctacttcata ataatctgcc
    10681 aatggctcac cagatacttc tacgccgttt aatcgactag gatctgagaa actttcactc
    10741 atcacgaaca cgatgttcgg cgactcttcc tctacagttt tatttttttc atcggctagc
    10801 ttctggtatt tttcagtgat ttctttgatc ttttctttcg aatatccttc aggttcatcc
    10861 attggttcta ccttcagatt gtacaaaaac ccaccgataa aaccagtgtt atagtagttc
    10921 attttctggc tgtaaggtat ccatagtgcc gttttattat atgcttttcg caacaaatta
    10981 tcgggattgt tgaaattact gatataccct agcagtccaa ttgccacaag cagtgtcgtt
    11041 aatcggatca attggaatcg ccttcctttt ttcaggcttc gaaacatgta ccagcaaaac
    11101 aatcctatca cggttcctgc ggcagctaag atcactgtaa acagcatagt gccggtcata
    11161 tctttcagca atccgatttc agtgatcatc ttcaaatcgt ctggataaat cggttcttgc
    11221 cgataactca ttttcatata atttgcaaaa cctaatacac cgatggctac gacatacgta
    11281 agggaaccta aagggacaga tcctgctagt gcaatcagaa aaataagaat aatcagcaag
    11341 acgagacagg ctaagaagaa tttttccgta tgccaagaaa aagcaaattt caacgcaagg
    11401 tctacggata gatcattttg acaccactgc aaaaataaat tactaaaaac aacaagaaaa
    11461 aagatactga tgattgctgc tgttcctttt aaaatctttt tctgtttggt cgaaagtctg
    11521 cttatatatc ttttcaaatg cttgctcctt catttctatc aataaaagaa tgttgcttct
    11581 cttattttag ttgtacgaaa ttcgaaagta aagaacggaa agaagaatta atgaaatcca
    11641 aatattctct ttctttatgg cctttctggt ttctttaata agcggacctt tcgtactcca
    11701 gaaatttttt ctagctgttg tttcacttga ttgagctgtt caaaatcagt actgtctatg
    11761 tcaatcaata tgtagacatg ctcattctta tggttacgcg atatatctgc gatcgctagc
    11821 tcgttctcat tcagcagaga aaatatctct gctaatatat tggttttctt ttgataaaat
    11881 atagtgaatc gaaacggcgc ttggaaaaac attcgtgcgg atggataatt gaccgcttct
    11941 ctaaccgtac cgaacaaaag ataatctttt aaggcttcaa ctgccaaacg tccgctgtct
    12001 gccaaagctt cctgagtcga tcccctagat gaggcaacat aagtatacgg tcattttcta
    12061 agaactcttc ttctggaaaa tcagtcaagt atttagccag atgttgtttt tccaatgctt
    12121 gcataacagc atatttatca acaatttcac ttcttcctac attgataagt acagcatctt
    12181 ttttcatttt ttctatattc gcctgatcga tcaatccttt tgtatcttct gtcaacggca
    12241 gcatgactac gatgtaatcc gaggtagaaa gaactgtttc taaatctgct tgccgaacat
    12301 aatccaactg ggcatcgcgg atagaataac ccaagacatc catccctaag ctatagcaac
    12361 ttagcgctac ttttgtgcct atcgctccaa gaccaagaag accgatggtt ttgccttgca
    12421 attctcgtcc cacataagca cttcttttgt tttctgcctg ttctaaaata ttaggacctg
    12481 ttagtgtttg taccatacga cttgcttcga ttatcggacg ggaagataag agcaaacagc
    12541 acaaaacaag ctcttttact gcattagcat ttacacctgg tgtattcatg acgatcgtcc
    12601 cgttttcaga agctttttca acgttgatcg tgttcacccc aacaccagct cttgaaattg
    12661 ctagcaaatc tggtgtaagc cattcatctt tcactttcgt actacgaata actattcctt
    12721 cggctggcaa atcatctatt attttgaaat attccccttg taacttttct atctcttgtt
    12781 ctggaatagc atcaatcaaa tgaacgtgat acataaaacc ccctccttac taacaagtat
    12841 ctcgaaattt ctgcaattag acaactgttt accatttgac ttcctagaag caaaaagagc
    12901 agttagagca gagtaggctg agtgataaaa ataaggctat gacaagttac tgccataagc
    12961 cttattttcg agagcaaaat attgagggtg agatatcttg ataaatggtg ttccgttagc
    13021 tgacattgat aataagacta aatttcaaaa aagttgaaaa gcaaatttcg tcaatctctt
    13081 cttatttctt aaaactaagt ggctttttca caacctcttt ttatctaata accactgttt
    13141 ccaactcaac taattttgcc cacgtttcaa tttgatctgt tgttagattt agcgagacaa
    13201 cggtatgatg accgccacct gcctgaatcc atgaatggac accttcatgg aaattcggtt
    13261 tagttttcca taacacacgt gcaacaggta aattaggtgc ttcagtcgtt ggttcaaagg
    13321 cttctacttc attgatcagc agtttataat gcgtaccgaa atctgccatc gatacgacta
    13381 ccccttctcc tgctttccca tcaaatacaa gacgcgccgg atcttctcta tctcccattg
    13441 ataaaggaga aacaacgatt cttggtttcg tcgcagcaag cgtcggatct acttccatca
    13501 tatgggattc caagattgct tcttggccac tagctagctc gtaagtataa tcttccatga
    13561 atcctgtatc tttccctcga gccatgattt tcagcaaccg atcaatcgct gcagttttcc
    13621 agtcgccttc cccagcaaaa ccgtatcctt ctgccatcaa acgttgtaca gctagtccag
    13681 gtagttgttt cattccatac aggtcttcaa aattagtcgt aaaagcgtta tagcctcctt
    13741 cttctaagaa acggcgaata ccgatttcct gctgagcttg tacttttaca tgttcctccc
    13801 atgtcttttc ttcataatca ccatagtcga aggtatacaa ttccttgtac tcttcaaata
    13861 attgatcgac ttcttcgtct gatacgcgat cgattacttg tacaagatct ccgataccaa
    13921 aataatcaac agtccaacca aattgaatct gtgcttccac tttgtctcct tctgtgacac
    13981 ccacgtttct catgttgtca ccaaaacgag ccacttttat tccaaaactt tcattatagg
    14041 ctactgcaac atccatccaa tcagcaatct ctttttggat ctcaggtcgt ttccagtagc
    14101 caacgacgat tttattttgt ttatttaatc gagcattgat gaacccatat tcgcggtcac
    14161 catgagcgga ttgatttaaa ttcatgaaat ccatatcgat cgttttccaa ggaatgcttt
    14221 cgttatattg cgtagctaaa tgaagcaatg gtttttgtag aagctttgtc ccgcggatcc
    14281 acatttttgc tggtgagaaa gtatgcatcc aagtgatcac acctgctact tcttctcgat
    14341 aattgacctc tttcatgatt tttgtgatcg tatcagcagt aactgctaat tcttgaaggc
    14401 ggattggata aggcaattgg ccattttcat tcagttcatc gaccatttct tgtgcatgtt
    14461 tctttacttc tcgcagtgct tcttccccat ataaatgctg tgatcctaca acaaaccaaa
    14521 attctttttc tccaatattc aacataatta gccactcctt ttatttgttt tgtccgtaat
    14581 acgcattttt tccgtgtttt cgtaaataat gtttatctaa aatccgttga ggcagctctt
    14641 ctgaaaatga attgagctgt ctggtaaata aattcatctt gcatacttca tctagtacga
    14701 ctgcgttcat taccgcactt tgcgcatctt tcccccaagt aaatggaccg tgtccgtgta
    14761 aaagtacccc tggaattgct aagggatcaa ttttgcgttc tttgaatgtt tctatgataa
    14821 cttttcctgt ttcatattcg tacccgctgt cgatttcctg ttgtgtcaaa aatcgagcac
    14881 aaggtaccga tccataaaaa gtatctgcat gcgtcgtccc catcgcagga acatccaatc
    14941 ctgcttgtgc ccagatcgtc gcccatgtcg agtgagtatg gacgattcct ccgatttgag
    15001 gaaatgaacg atataaaaca gcatgagtag gcgtatccga agagggattg agtttacctt
    15061 caatgatatg attgtccaag tccacaacga ccatgtcgtc tgctgtcatc tcttcataac
    15121 tgactccgct aggtttgata acgaaatatc gtgtttcagg atcaaaagcg ctcacatttc
    15181 cccatgtata tttgacgagt ccgtgtttag gcaagtcaag atttgcttgg tatacttctt
    15241 ctttcagttg ttccagcatt ttctatcact cctttttctt gcaattgatg aatcgctgtt
    15301 ttttcaatgt cgatcccctc acggtaacgt ctcataaata tctgatagcc tgctatttct
    15361 tctgctgaag gtgtgatggt gattccttgg tcttgtccaa acacgtcttc tgccaagaag
    15421 ttttctaaag acttcccatg tcgatccatc atataggctg ctaataacgc gattccccac
    15481 gctccacctt ctccggctgt gtccatcact gtaactggtg cctccatagc agatgctaga
    15541 atccgctgtc caacttctgg tgttttaaaa atccccccat gccccactaa acgatcaatt
    15601 tggacattct cagatttcaa tatatccatt ccgattctca ttgcaccaaa agcactggct
    15661 aaatggatcc gcatgaaatt cgctaaatca aaacggctac ctggtgtacg tacaaataat
    15721 ggccttcctt catttactcc tgtaatgttt tcaccggaaa aataaccata agaaagtaat
    15781 cccccgcaat ccgtatcgcc ttctagcgct ttgttgaata ataccgtgaa taatttatct
    15841 ctgcttatct ccatacccaa agaattggta aattcttcaa aaatactgac ccaagcattg
    15901 atatccgaag agcagttatt cgtatgaacc atgcctacta gttctcctga aggtgtagtg
    15961 accatgtcga tttccggatg gacattttct agttcttttt ctaatacgat catagcgaag
    16021 gcggaagtac cagccgaaat gttccctgtc cctttggcta cactgttcgt agcgaccata
    16081 ccggttcctg catccccttc tggcggacaa accgggattc ctggtctcaa atttcccgta
    16141 gggtcgatca gataagcccc tgattcgctt aacttgccag cttcttctcc tgctactaga
    16201 actttaggca aaatgtgatc caagtcaatc gctacgtgcg ctttttcgat taactgacca
    16261 aaaatatttt tcatttcttg atggtaggtt ttcgttgaag gatcgatagg aaacatgcct
    16321 gatgcatctc ctacgcctag tactttctca ccagtaagct gccaatggac ataacctgca
    16381 agcgttgtca aaaaattaat ttctttcaca tgttcttctt tattcaaaat agattgatac
    16441 aaatgagcga tactccatct ctgcggaata ttgtattgaa atttttccgt taatattcgt
    16501 tctgcttctt cagtgattgt atttcgccaa gtacgaaatg gtactagtaa ctgttccttg
    16561 ctatcaaatg ctagataacc gtgcatcatt gcactgaacc caatcgaccc gattgtcgta
    16621 agattctcgc catagatttc gaatacttct gctgccattt ttcggtaact gacgcgcagt
    16681 cctttccaaa tatcttctaa tgaataggtc cagataccat tttctagttt gttttcccac
    16741 tcataactac cagcggcaat aggagtaaaa gatgaatcaa tcaatacagc tttgatccta
    16801 gttgatccaa actcaatccc taaagatgta cgaccatcta tgacatctaa catactttgc
    16861 gcatttactt ccttcataat gatttaactc cctccttctc gtcaaacctg atcaaaccgt
    16921 ctttttagta gcgcttacaa tgattattgt atacttttgt acgtacatgt caagcagtta
    16981 tttacaagaa tacaataaaa aacacttgtt ttgtacgtac ataagttata aaatgaaatt
    17041 gcttacaaaa tctgatcaac cgccttctcg aaattctaga aggagagaat acttatgaaa
    17101 aaagaaaaaa aagtttcctc atcgtttatt tatttttttg gcgcatttgg cggcattcta
    17161 tttggatatg atattggtgt tatgaccggc gccttacctt ttttacagca tgattggggt
    17221 ttggcaggta aagcttcttt gataggatgg atcacgtctt cggtcatgtt aggtgcgatc
    17281 cttggcggct ctttatctgg tcttctttct gataagcttg gacgacgaaa aatgattctt
    17341 ctttctgcac ttatttttat ggctggttct gtcctttctg catctgcccc tcacaatggt
    17401 tcttattttc tgattgcagc aaggattctt ctaggtttag ctgttggcgc tgcatctgcc
    17461 ctagttcctg cttatatgtc tgaaatggct cctgctagac taaggggaag attgtcaggg
    17521 atcaatcaag tgatgattgc ttctggcatg ctcctttcct atgttgcaga ttatttgcta
    17581 aaagggttgc ctgaaacgat ggcttggcgc gtgatgttag ggctcgctgc tgtccccgcg
    17641 ctgattttat ttttcggtgt tttagctcta ccagagtcac cgcgttttct tatgcaatct
    17701 ggccgcttgg aagaggcaaa aagagtattg aactatattc ggacaccaaa agaagctgaa
    17761 caggaattcg aacaaatcca gctgaatgtc aaacaagaaa agacaaccgg aacttcttgg
    17821 catactttgt ttttagaaaa atatcgttcc ttagtcattg ccggtattgg tgtagcagta
    17881 ttccagcaat ttcaaggagc taatgctatt ttttactata ttccgctgat cgtagaaaaa
    17941 gcgactgggc atgcagcgag cgatgcattg atgtggccga tcatccaagg aatcatttta
    18001 gttgctggtt ctttgctctt tttggttatt gcagacaagt tcaatcggcg aactttattg
    18061 aaaataggcg gtagcgtgat gggactttct ttcatcctac cagctgtttt aggaactgta
    18121 ctggatgctc atacaaattc gttgttgatt ctcttgtttt tatgtattta tgtcgcattt
    18181 tattcttgca catgggctcc actgacatgg gtaatcgtag gcgaaatatt cccactagct
    18241 gtccgcgggc gagcatctgg cctagcctct tcttttaatt ggattggttc tttcctagtt
    18301 ggcttgcttt ttcctgtgat gactgcttct atgtctcaag aaatcgtttt cggcattttt
    18361 gggatcatct gctttttagg ggttttattt attcaagaga tcgtaccaga aactcgtggg
    18421 aaatctttag aggaaatcga acaaagtgca tctaaaaaaa cttatcctaa acgtatttct
    18481 atccatcatt cttaaagagg ttgtgacaga agtggtcagc ttcaagaaat aagaaggaac
    18541 cgcttagttt ccagtcacaa ggaacaattt caaaaaacag cttctcatgt ttgatgaata
    18601 ttgtgacttg tgaccagttg gtaggagaac accgtttagt ggagaggtgg tgaaagaaac
    18661 gttcagcctt gagcgatgac aaaagctctc acaaacattg cgacgagtaa ccgtaggagc
    18721 aataagaggc ggtaaaaacc cgtctcttat ttttcgtttt ctttcactgt ttcccgttca
    18781 actagtacag gttcatagag aatatttggc tgtgcctgtc ctttttcaat cgttgtgacg
    18841 atccaccgtg cggcatcttg tcccaacttt tcttttggat gcgtcaatgt tgtcaatttt
    18901 ttattcccta aatggcttaa agaagaatcg tctgttccta cgatcgatag gtcatctgga
    18961 atatgatagc cattttctgt caacttatct agcattaaac tagctatttg atcgttatag
    19021 caaatgatgc catctaaagc ttgttcttct ttcaaccgtt gtactgtttt ttcagcgatt
    19081 gtttctcgtg tttccgtggt gaaagtaatg atatcttccg tcaaaaaacg cacattatac
    19141 tgttcacacg cgctgataaa acctttcatc ctgtattttc cttgaagatc gtcgattttt
    19201 gtgatgaaca acagacgctg atgtcctttt tttatcaaat attctgctgc cataaaaccg
    19261 actttcacat catctaaaca aatataagag gcatttaact cttcataagc agcattgatc
    19321 atcaccatcg gaatgccttg ttctctcaaa gcaacataaa gtgctaagtt tgggttatac
    19381 tgattacttt tcgtaggctc aacgatcaaa ccatctaccc cataagcaat cattttcttc
    19441 aaacactctt tctccaattc gtgatcgtta ttcgtactgg ctaaaagcaa tgaataccca
    19501 gattctcgta attcattttc gattcctcgg ataatcgacg gaaaaatata atcagagaga
    19561 tatgtggtga tgacaccgat cgtttttgtt ctgttcaatg gtgttttttc tttcttatag
    19621 ctgtcatcta catatgtgcc cgaccctttt tctttacgca gatagccctc atttaccaac
    19681 acagcgattg cttgccgaat cgtatgtctg ctgacgtcat atatttcttg cagcttgaat
    19741 tctgagggaa tggttgtatt tacttcgtat tcctcacgaa ggatcttttg ttttatctct
    19801 tctgcaatat cttgatattt tgccatttgc cttgcccctt tttatttgta cgtacatata
    19861 tcataacgag tctgcaaggt ttattcaagc aaaaatcaag gtttcttgtg ctcataaggc
    19921 atacggaaca ggttcaccaa attctggaaa tccttgctca ttccacgtaa agcattgcgc
    19981 gttcgcgtgc cgattaggat tgtctaacgg atcccctttt tggttcttct ctggtcgagc
    20041 atgatagatc aaagtatcaa agcacccgtc ttctgaaacc gtaaacgagt tgtgaccagg
    20101 accaaattgc ttattttttt cagaagaaac gaatactggc tccgataatt tatgccatga
    20161 ttcttttaat aaaagattct tgtgctcatc agcccacagc aggcccatgg catagttttc
    20221 atctgttgca ctaccagaat acgtaatgaa tattttccca ttgcgagtaa ggactgctgg
    20281 accttcattt actgaaaaac cccgtttttc ccattcatac tcaggtatag aaaggaggat
    20341 ctgcttcccc tttaacgtcc aaggattatc catttcggat agataaagat tagaattccc
    20401 agtaatttca ggatcttgtt gggcccacac ataatatagc tttccttgag aagagaaagt
    20461 tgttccgtct aaactgaaag tttccctttt cgtttttatt tgtccctttt ctatccaatc
    20521 tgtattcatt ggatctatat gctgattttc aagtacgaac atgcggtgat gatgagattc
    20581 atcacgtata gttttatgat tgcttgcagc aaagtaaata taccattttc cattgatata
    20641 atggacttca ggtgcccaga tcaattcact catcggtcct tcttcatgag cctgccatac
    20701 aatcaggctt gcagcatgtt ctaattcatt cagtgacttt gcccttctta attcaatcac
    20761 ttggtagcct ggaaccgatc ctgtaaaata ataataacca tctgtatgtt tatataccca
    20821 aggatctgcc cgttgccgaa cgataggatt attataaagt gtcttcatca taacaaattt
    20881 cctcacattt tctttattct aattacattc caagagtatt tttctatccc tacatttaat
    20941 tcgtcatgtt tttctattga aaaatagttt atttcaggtt ttattttttc tggttcttca
    21001 cgtgtatttt tgtccaacag atcgctactg gttaatgtgt agtgctctcc ttgatctagc
    21061 aaattgaaat catgttcaat cgttatcttt tgttgttcat ttgtgcgatt gatcaagaaa
    21121 atagtcagct gattgccgtt atctacgatc acactgtcca cataaggaac attttttatc
    21181 ttttcacaat cataaacagg gcaaatgatt tgttgtttca atacttgacc tttagcatat
    21241 ttcgataagt gttgcatcac ataataagtt ggattcctcc atgcttgtcc tttagcatct
    21301 gtcagtatta aaggtattgt gtttactaac aatgattgac aactaatctt tacgcgatct
    21361 gcgtgtcgta aaatagccag ccctaatgaa ccaaataata atgtatcttc catagtaaag
    21421 taacaccaat cgatgggact accgacttca aaatccctat gtcttttttc cggttgggca
    21481 tgtacattcc attcatcaaa cgctagatac attgttttct cacttctcaa atatcctttt
    21541 acataatcac aagtagcaac gatttcttca atttgacgat cgaatcgata agcttttgca
    21601 aggtatgtcg ctgtatcatc acgctcattt ttaggaggtt tagtcaagtc ttcttcatca
    21661 tacttatcaa tatagttatg caaagctaaa taatctacat tctcataagc ttcttctaat
    21721 gcgatacgat cccattcagg gtacgatggt aaacgtggcg tagagcttcc aactaagatt
    21781 gtttcaattg aatcatccac taacttcatg acttttgagg cttcatttgc taacctacca
    21841 tattcataag cactcttagc ccccacttgc catggaccgt ctagctcatt ccctaaacac
    21901 cataatttaa cgccaaaagg ctttttttgc ccatttttta ttcttaaatc actccagaag
    21961 gttccttttg gaaaattaca atattctact aaattttttg cttcatcaat tccttttgtt
    22021 cctaaattca ccgtgaatat cggtttcgct tgtacatgac tgatccattt aaaaaattcg
    22081 tggagtccaa aagaatttgg ttctatacct cgccatgcta aatcaatttt ttttggtcgt
    22141 tgttctttcc tacctacagt atccacccaa tcataaccag atgtaaagtt tcctccagga
    22201 tagcgaatga cacctaattc taattccttt acataagaga ggacatcttg tctaaacccg
    22261 tcaccatcag ctgtttcatg gcccggttca taaatccctg tatagacgac acttcccata
    22321 tgctcaacaa atgagccaaa cagccgagaa ctgatctctc ctaccgtaaa atcctctctg
    22381 atttttactt tcacttcttc gtccaacagc tttccctacc tttcatttcc tatagtcaag
    22441 ataatagata caagtttcca tacgccacca gcaaagataa aaataaaagg agggaaaaga
    22501 aatatgagca gtacaaatcc taataacaat acgcaacaaa gaatatttct caaaaaatca
    22561 aaaaatactt tccatgcact ttgttcaaaa atttgtaaaa atgtgatatc tttgttttca
    22621 gaaatcacat atatccagga taaaagaaac atggaataaa taagtaaaac tacatatcca
    22681 ctaattgaaa gtacgattgt ctgttcttct aataattgat taacaagaaa ataaataatg
    22741 atcgttaaca gactaaggaa caagttctta ccaaaatgtt gctttattcc ttgaaagaaa
    22801 aaataaatga tatttcctga tccctcacct ttccattgcg ccattacata aaaaacagca
    22861 catgtagcag gcacgatagt aataacaggc aaacacacga ttacccagag aatacctaat
    22921 aagatgtaat cagaaaccac aatcaactgt ccataaagtt tagtctcaac tattttcatc
    22981 ttcctcactc tttcattcca gacatagctg ttccttgaac aaaatatttc tgtgcaaaaa
    23041 tataaaccac tagtaatggc agcattgaaa taaccgttcc agccattaca ggtccataaa
    23101 aagaaatgtt ctgccctgta aataaagcta accctgctgg taaagttctc atttttgaac
    23161 tacttgtcaa tatcaatgga taaagcaagt cattccaact attcatcagc gtgaaaatcc
    23221 ctaatgataa aagagtaggt ttgctcaatg gcaacatgat tttcaaaaaa atattaaact
    23281 cacttaatcc atcaatgcgt gctgcctctt ccaaagaatc tggtaaagta ataaaaaagg
    23341 aacgcatcat gaagatacca aatgcagttg tcatacgagg aatgatcaaa cctgcataag
    23401 tatcaagcat gccaagcaaa ttgacttcaa taaatagagg aatcatgaag acttggaatg
    23461 ggatcatcat tgtcagcaaa acgaaataga acaaaactga ttttcctttg aaacgcatac
    23521 gagcaaatgc ataccccgca agagaatcaa agaatacaga agtgatcact gtccctccag
    23581 caaaaatcac tgtatttttg atgtaatcta ataaagggat cgttgtccaa accttaatat
    23641 agttatctag ggtatagcta tctgcaaata ttctaggcgg aaatgagata atgtcttttt
    23701 cgtgtttaaa agaagaaata atcagccaca aaaaaggaaa cgcaataacc aacaatataa
    23761 ctagcatact aatcgttcga atgatttttt taccactaac tgcttttcga ctattcacag
    23821 taactgtaat cgagctttct ggtgccatac aggtttccct cctatttcat ttgtttttct
    23881 ttccgtgaca tgtacacatt agtggagata gctatagttg caacaatgat aaacaaaacc
    23941 gaagacagtg ctgatgcata gcccaattca taaggtgctg taaatcctcg actgtaaata
    24001 tattgaacag ctgtttctgt cttaaattgt gggccaccag cggttgcaac atatacttgg
    24061 tcaaacactt gcatcgaacc aatcagattc gttaaaatac aaaaacctaa ggagggaacg
    24121 atctgaggta gcgtaatgtt aaaaaactgc tgtcgatttg ttgcaccatc catttgagca
    24181 gattcataca aagagggaga gattccctga atagcagtca agagaatcgt catagtaaca
    24241 ccaaaattct tccaaacagt catgatggct accgtcggca tagcaagggt tggatcgcga
    24301 aaaaattgag ggatcggcaa gccgacttgt gaaaacaagt aaggaatcgc ccctacattc
    24361 ggatcaagca acatagaaaa aataatcgca attgacgtca atgaacaaat aactggaatg
    24421 taaaatgtcg aacgacaaaa acgattgaat ctggtatttt tagataatgc tactgctgct
    24481 aataaaccaa cgataatttg tgttggcgtt tcgagtacgg tgaaatataa agaatgaatc
    24541 aacgaattaa ctgctcgttc atcactaaat atacgtgtga aattttcaaa acttacaaaa
    24601 tttcttgatg taaagaaaat attcaaatct gtaaaactaa tagcaaatgt tcctaaaagc
    24661 ggtatgacag taaataataa taaaactaaa aaagatggag ttaaaaacaa atacgcagca
    24721 cgatcaggac gattccaata tttggtgaat ccgttcatat agtccacctc atctttcatt
    24781 ttgataaaat atacaatctt ggtttaaacg aatctgaata agatatccga acaatcgctg
    24841 tgatttttgc aataacacct atctgcaaat actaggtcga tcattcagat atctgtatct
    24901 tgccaacagt ctatcctgta taaagttctg tactattttt gcagtaattg attgatggca
    24961 tcatctgcat cgttcatgag tgtctcagga tcttctcctg ctaataattt ttcaatcagt
    25021 ggattgatga tatcgttatt gatcgcagag atttgggtaa aacctggtaa atatggctct
    25081 gcatagtcca tttgtttcga taattcagat acaataggat cattttttac ttcttcatct
    25141 tttgctactg aatccaagta ggcaggaaaa ccattttcga tactccattt tttaccaatt
    25201 tcagttgtat tccaatattt caaaaactca tagacagctt cttttttatt attatctgta
    25261 cttgccggaa tggcatatcc tacgccatct aaaatagctg tttttttgtc tgaatcactc
    25321 ccttgtggta aggtagtcac attataatca atctcgtttg ctttcaatcc gttatttaac
    25381 catggcccat tgatttcaat ggctagttga ccagcattca ttaaattgtc catttcggcg
    25441 ccagaaatcg attctggacc agtcttgtct acatggatca ttttttggat cgcttttaac
    25501 gttttaaggt tctcaggaga cgcaaattca gacttagtat aatcatcgtt cactaattta
    25561 ccgccatttg ctaacatcca gttatataac ggtgcagttc catccttatt gaagacaaaa
    25621 cctgatacat ttttactact atcagttaat tttggtgcca tttctgtcaa ttcttcccaa
    25681 gtagtaggtg gttgttcagt atctaatcca gcttgtttaa acaaatcatt attccaatac
    25741 atatacatgc cttgtacctg catggggaca aaatactgtt taccttctac ttcacctaat
    25801 tcaatagccg tttctttaaa atcagattta tctactccat catattccca gaaatcgtct
    25861 aatggttgta ccgctccgtt ttctacatac tgtgcaaaat ctggataatt taatgcaata
    25921 aagtcaggtg cttttttaga agaaatcgct gtaggaagtt tttcattcaa attggcccaa
    25981 gtcatgatat ccatttcaat tttgatgttt ttatcatttg ttttattaaa ttcattaacg
    26041 atttcttgca atatttcacc atcagatgca gtaaacccat tccaaaaagt caaagtcact
    26101 tctttatcag aatcagattc tgctgaactt ccgccaccgc aagctcctaa aatacctgtt
    26161 gcagctacca acaaagagcc aattaaccat tttttacctt tcattttttg ttcctccttc
    26221 atctgataaa cgacatctga ccacattcca agatagcggt ttcaataaac aattgatttt
    26281 cccatcttca gcttttacat ttgttgaatg acttggtttt acttgttcac ttttaggaag
    26341 attagttgct tttgtatcaa atccactcat ttcagaaaaa tcaatcactc cctctaatga
    26401 taattcagac aagtgaacag ttagattcat ttcttcttct ccacggttaa ctgcaaatat
    26461 gacaagttct ttgtcattta ttacggctat actttctaaa tacggcactt cttcaaaatc
    26521 ttccgtcgca tagtttgctg aacgaatttg tgggcttaac acctctcctt gtccatacac
    26581 agcagcttgc ataaatggat aatagatcgt ctgtctccaa gcttctccgt ttgtttctgt
    26641 cctgattggt gcaataacat ttaccaattg agcaagacaa gcaatcttta ctctatctgc
    26701 atgtttcaat aaagtgatta atagacagcc taatagtaat gcatcttcaa aattatagtt
    26761 atcttctagt aaaggaggag caatctgcca aggcgctgct tgctcatctt gttccgctga
    26821 atgataccaa atattccatt catcaaagga caggttgatc tgcttcttac tatgtttctt
    26881 cgccttgact gtatcgcaca tcgctgtcac ttcagaaatg aaccgatcca tgtctaagga
    26941 acgtgcaaga taatgagaaa gatcattttt ttggttgcca taatattgat gcagagacaa
    27001 ataatctatt tgatcgtacg cttgatcaag aactgttaac tcccaatcac caaatgtagg
    27061 catttgccga ttcgaacttc cgcataaaac cacctcaatt gaatcatcta cgagtttcat
    27121 cgccttagcc gtttcctctg ctaaccgtcc atattcttcc gctgttttat gtccaatctg
    27181 ccaaggaccg tccatttcat tccctaaaca ccatgtctta attccaaaag ggctctcttg
    27241 accattcgaa cgccttaaat cactccaata agttccttta ggaaaattac aatattcgac
    27301 taaattacga gctgcatcta ctccacgagt acccagattg acagccatat tgatttcggc
    27361 ccccacatgc tgacaccagt tataaaattc atgaatccca acttcattcg tttcaattgt
    27421 ccgccaagct aaatccaacc ttctaggtct atttgctatg ggcccaatac tgtcttccca
    27481 acgaaatcct gacacaaaat ttccaccagg atatctaatc atgggaatgt tcaattcttt
    27541 taccaagtga atgacgtctt ggcgaaatcc acttttatta gctgatggat gatctggctg
    27601 ataaatccct tcatatactg ctcgtcccat atgttcaata aatgaactgt acaaccgcgg
    27661 atcaactttg cctacacgct tgacactact aatcaacatt gtggcttcca taactgacct
    27721 cctttaattt atacatgaat aatacacctc gttgaaagcg ttgtcaataa tataaatgtt
    27781 aaacttactt attttttatc gatttt
//
LOCUS       DAZKLD010000019        27786 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.019, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000019 DAZKLD010000000
VERSION     DAZKLD010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27786)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27786)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27786
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.019"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..676)
                     /locus_tag="ACG8KR_000718"
     CDS             complement(<1..676)
                     /locus_tag="ACG8KR_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016611336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFQ0200116.1"
                     /translation="MKKIAKYMEIYLDIKKKIVNGEYKIGEKLPTGDELAVIYHTSKL
                     TVKKGVDLLVSEGVLRSRSGYGTEVVRTPIDNSRVLGPNEGLFSVVGEEHVDSEIHTF
                     SIELPSKKVADKLSISVKDYVYNIVRSRFIDCQPYSIEQTFMPLAVIPGLEPKHLKKS
                     VYAYITNELGLEIKTSHIRIKGDTANEFDAKILKIDPGEFMIEIDKVVALSSGKPFEY
                     SVSRHLY"
     gene            851..2275
                     /locus_tag="ACG8KR_000719"
     CDS             851..2275
                     /locus_tag="ACG8KR_000719"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFQ0200117.1"
                     /translation="MTTMKKLTFPDDFWWGSAWSAEQAEGKGETGKAETIWDRWFKEQ
                     PYRFYQRIDSSITTDHIHQYKEDIELMKKTGHNSFRVSISWARMFPNGGTGKVNPKAI
                     SFYRKLFSEMNNNGIKVFANLYHFDMPATLQDKGGWESREVVDAYVHYAETCFKEFGD
                     LVCHWFTFNEPLGPILGTYLEDFHYPNLIDFKRGAQAAFFTILAHAKAIQVFKELALP
                     SKIGVILNLSPTFPRSQNPADVKAAEIADLFYTRSFLDPMVKGNFSKKLILLLQEYNQ
                     LPEDYTKEDLVCIYENTAQILGLNYYEPRRVKSRSSMVDTSSPFLPEWFFEPHIMPGR
                     RMNEYRGWEIYEKGIYDLCMDIKNHYGNIEAFISENGMGVADEERFMDDTGQIIDDYR
                     IEYIKDHLAYLWKAINEGCNIKGYHLWTFIDCWSWINAYKNRYGLVSLDLTTQKRTIK
                     KSGEFYKQLSDTNGFRYDVDLLVE"
     gene            2574..3281
                     /locus_tag="ACG8KR_000720"
     CDS             2574..3281
                     /locus_tag="ACG8KR_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HFQ0200118.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLIYAGSAQFITVSMLASHSPLLSIVLSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVILV
                     VTLIGCGLGVWIKHAFF"
     gene            3268..3591
                     /locus_tag="ACG8KR_000721"
     CDS             3268..3591
                     /locus_tag="ACG8KR_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HFQ0200119.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRILPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLVGILSLS
                     LIRLL"
     gene            complement(3680..4717)
                     /locus_tag="ACG8KR_000722"
     CDS             complement(3680..4717)
                     /locus_tag="ACG8KR_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HFQ0200120.1"
                     /translation="MSEKELMKVVGFYEGLSIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTTRAG
                     SNQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKIL
                     VINGAGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELG
                     IDQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMF
                     AKTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHIL
                     GKVVVSGPFNG"
     gene            complement(5038..6021)
                     /locus_tag="ACG8KR_000723"
     CDS             complement(5038..6021)
                     /locus_tag="ACG8KR_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase"
                     /protein_id="HFQ0200121.1"
                     /translation="MTTNFWKELPKPFFVLAPMEDVTDVVFRHVVKHAAAPDVFFTEF
                     TNSDSFCHPDGKDSVRGRLTFTEDEQPMVAHIWGDKPEFFREMSIAMAEMGFQGIDIN
                     MGCPVPNVAERGKGSGLILRPEVAAQLIEAAKAGGLPVSVKTRIGYTEMSEMEEWISH
                     LLHQDIANLSVHLRTRKEMSKVDAHWEVIPKIIALRDQIAPQTLITINGDIPDRQTGE
                     ELAEKYGVDGIMIGRGIFKNPYAFEKEPREHTEKELIGLLRLQLDLQDHYSEIIPRSI
                     TGLHRFFKIYIKGFPGANDLRVQLMNTKSTDEVREILDTFEKEHPTLFSEQ"
     gene            complement(6436..7206)
                     /locus_tag="ACG8KR_000724"
     CDS             complement(6436..7206)
                     /locus_tag="ACG8KR_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0200122.1"
                     /translation="MFNLYFTALKSLAIKETNRYLRIWVQTLVPPVITTSLYFVIFGK
                     MIGGRIGDMGGFSYMEFIVPGLIMMSAITSSYANVSSSFFSQKFQKNIEEILVAPVPT
                     HVIIWGFVIGGVGRSILVGSLVTIISLFFVPLHVYSWFMVIITLLMTAILFSLAGLLN
                     GIFAQSYDDVSIVPTFVLQPLTYLGGVFYAISMLPPFWQAVSKVNPIVYMISGFRYGF
                     LGTIDVPVATSMIVLVLFIVVLYAVTWYLINKGRGLRS"
     gene            complement(7199..8134)
                     /locus_tag="ACG8KR_000725"
     CDS             complement(7199..8134)
                     /locus_tag="ACG8KR_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0200123.1"
                     /translation="MANALEINNLKKVYGSGVEALRGIDLSVKEGDFYALLGPNGAGK
                     STTIGIITSLVNKTSGKVNVFGYDLDTDLVRAKQQIGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVSRKEAMKRSEKYLKQSNLWEKRNVRARMLSGGMKRRLMIARALMHEPRLLIL
                     DEPTAGVDIELRREMWEFLRELNENGTTIILTTHYLEEAEMLCRNIGIIQSGELIENT
                     SMKELLSKLQFETFIFDLAPYETKPVIEGYACTFEDEQTIAVEVERNQGVNGIFDQLT
                     RQGIKVLSMRNKSNRLEELFLKITEENNHTEEKNV"
     gene            complement(8450..10099)
                     /locus_tag="ACG8KR_000726"
     CDS             complement(8450..10099)
                     /locus_tag="ACG8KR_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HFQ0200124.1"
                     /translation="MGQREFIVLIIIAAVILLVALDIFSRLKLKETVRSKWEKIPYQP
                     RFDKEESLKEAWLTEKKFRSWDSEIDDLTWYDLDMFEVFEGINSTYSSVGSEALYQRL
                     RSFDFGEDQQLEKLIAFYQENPQLREKIQYQFARLGKKDHNFAKQYLADGKSKKIGRV
                     GLFIFLGLLPLVGLGLLLLGQVLGIYLAIGGLVFNTVYYLVKKQTLETELNSMSYLVS
                     TISAANQLAKLPTPLQEELKQQTKPLRDIPKLGFSFRMKSGSEAELIFEYVNIMFMLP
                     FISYHFVLTKLEKHSTEAIKLWELLGELEVAAAILNYRTYMPITCRPEFTEGGVTAKD
                     IYHPLLKEAVVNPVNWQKNTLVTGSNASGKSTYVKSIAISCILAQTIQTAIAEQFTLQ
                     PGHVLTSMAIEDDLFEGDSYFVSEIKSIKRLLDQVASKERCYCFVDEILKGTNTIERI
                     ASSASVVRWLAEYPSLAFVATHDIELTEILKNYCENVHFEEQVTEGKGISFDFKLKEG
                     PSRTRNAIALLKVLDYPETLVETAKTEAALFDEKRQWHVLG"
     gene            complement(10216..12819)
                     /locus_tag="ACG8KR_000727"
     CDS             complement(10216..12819)
                     /locus_tag="ACG8KR_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HFQ0200125.1"
                     /translation="MVNAHIIQHTHWDREWYFTNEDAIVLSDQVFTEVLDELERNPKV
                     NFCLDGQTSIVDEYLEINPQKLKVIQRLMKTGQLFVGPWYAQTDALLVDAESILRNLM
                     IGISDTILKYGDPMMVGYLPDTFGFNANLPTLLHQVEIDNFMCWRGLNFETMVPSPYF
                     IWKGLGDKFVYAMNFPFGYMTGMMTLDAIKDIPEFAKEKLDEAVEMLHMQGKNKDILV
                     PSGVDQKSMILNFDQVVEKLNKYSKFHNIISDYPSYVEVIRNKKELPEYKGELRQPVY
                     SRVHRSIGSVRTKMKIENFKLEQKILKRVEPLMLIAERNGIHISKGLLRRLWKKILQN
                     QAHDSIGGCVSDNVAEDIFHRIKEANEIADGIENLIVKRMADALKLKSNEVLVFNTTT
                     ENYSGSKIVHVVADSKKIHFKGSPYNIIEKEVYFPERKNAYRMIATGFEYFDEPAYYE
                     LDIKINVNVPALGYTVVEFEKNNEKLETAIEIDKTEISNNKYKLSFKDGQLNLIVGDR
                     QYLDFVHLIDSANDGDTYDYSPLEGDTELSLKLETAKVYKDSLQETLVVYGKAQLPKD
                     LKDRLSEKPEMEEISYEISFSLGESQIVEGTLKIHNTVYSHRMRLAINVLDKSEKSIA
                     QIQNGFIENTPDVIPPNWSETMVEKPVNLEIFDKSVSVAENDHYFTFYADGLKEYERI
                     ENQLIITLFSTTGELGKPNLAWRPGRASGDTTNEGHVMMETPLAQEIGEYKVTFGFNV
                     EEGRFNEFKVAKQAEKRLEQSISYQKQKLNVFIHRLDNKIWPLQNELDVPRKFSLIEL
                     PEDLIVSSIYPSYFHEQGFVLRLANPTEQEKIVPEAILSLGTVVNALENKQELTTIPP
                     YDYLSILINER"
     gene            complement(12831..13880)
                     /locus_tag="ACG8KR_000728"
     CDS             complement(12831..13880)
                     /locus_tag="ACG8KR_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFQ0200126.1"
                     /translation="MEFKKQGREILNHFQSGVSYMIPLVVAAGLLTSIAVIFGGTGVW
                     DQTDTFWGVLRMIGQTGLQFIVPMISAYIAYSIADRPGLAPAFITGMMCQNLGMGFIG
                     GMVAGLACGYLANALKKVKVPMQVISLKSIMLIPFVTTLVVGIILWYVLATPIQAMTT
                     GLTNWLEGMSGANAALMSAILGGMMAFDMGGPINKIAYAFGVASFSSGSFGPSTAMLL
                     GIAIPPFGMFLATILNKKLYDESERDNAKTAMIMGLVGITEGTIPFAVKDPLRVIPSI
                     VVGTAVACGLNGFFGVTQETMLSTFMAIPFVTNIPLYLVSIVIGGVVTALMVNALKSI
                     KYKKEQQVNSVEEGK"
     gene            complement(13906..14220)
                     /locus_tag="ACG8KR_000729"
     CDS             complement(13906..14220)
                     /locus_tag="ACG8KR_000729"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_function: GO:0090563 - protein-phosphocysteine-sugar
                     phosphotransferase activity [Evidence IEA];
                     GO_function: GO:0090582 -
                     protein-phosphocysteine-D-fructose-phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HFQ0200127.1"
                     /translation="MTKIVAVTACIAGIAHTYMAKANLEKFAKKKGLDIKVETQGAMG
                     IENKLSISDVSEADVVIFAVDTNVSERGRFDGKPILTVGTSEVVKDGEETIDEAIALI
                     NK"
     gene            complement(14236..14682)
                     /locus_tag="ACG8KR_000730"
     CDS             complement(14236..14682)
                     /locus_tag="ACG8KR_000730"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose PTS transporter subunit IIA"
                     /protein_id="HFQ0200128.1"
                     /translation="MISEKININNIVIDLNASDKKSVVEKLSKLLETNGFVSDHKQFQ
                     KDVFDRENHMTTGIGKNIAIPHGKSLAVIESTVAVAKLKRSVEWGALDDQPVDLVFLL
                     AIKDTDKGTNHLRILAELSGRLMDDEFVKRIKDSRNKKELVKALSF"
     gene            complement(14683..16605)
                     /locus_tag="ACG8KR_000731"
     CDS             complement(14683..16605)
                     /locus_tag="ACG8KR_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFQ0200129.1"
                     /translation="MGIEFSERQMKILYFLIENEHTTYKELSEQLYVSTKTISKDILI
                     LKEIIQKMPDVQMLVQPKKGVSLIGKKETLRNLLSSHKINIADSPEDRLTYVFAKLLN
                     TNDSLKIQNLADELYVSRSTVESTMKDVRKRLNKHGMSLVSDRNGVRIKADEKSKRRL
                     MSEVVNYYWGGFTASNSKTDLLDLNINFIGEANNFLNKDILKKVAELLNRYMNKTQLK
                     FTDYEFQTLAIHLAIAIERIETSNFLSRSNNSEEEISKNTLILIKMIEKAFSIIVPID
                     EQAYVDIHITSIEKNAMNHDLDQTKLSYLDMGVELQELVRIKLNEFYPDEELINNLVL
                     HLNAAVKRLKLGVNIYNPYTDKIKYSFKRSFMISVDLLEEIEERFCIHFNEDEIAYVT
                     LHIQSLLDRYKPDKTKVILVCSSGYGTSKLLEQRINNGFADMVEIEDVLSINELQDRK
                     VTDELVISTIPIENTNFPVIVVSPLMLGADVRKVKQYLQLNEQTEDAFLNLLDKRFCF
                     FTHQIKDNQVNVLNIITKKLVKENYAKEGILEGAIKREKLSSTAMNDFAMPHIEVDFI
                     KKPVICVYVNPEGIDWDGTLVHSVFFFALNEKVKPELNKIYETFNEIASNHELLANLK
                     TSSSFEKLVYILKEGL"
     gene            complement(16817..17713)
                     /locus_tag="ACG8KR_000732"
     CDS             complement(16817..17713)
                     /locus_tag="ACG8KR_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFQ0200130.1"
                     /translation="MIKWLLLLVVIFMGSFIFFLQREIKRLTKKIATLSERQEYGGRL
                     SVEFREKNLMDLVDALNTWLDENEQKGQAFQEMEENIRLSIVGLSHDLRTPLTAINGY
                     VQLLEQVEDTEKREKYLTIIKQSVARLLSMTDQFYDLARIETKQKEMDLHTLSFSKEV
                     EEVFFSFYEQFSEKNIEVAFAEHSINTTVLADPLMLLRVLQNIIQNLLRYADGQAKVT
                     FSKEDPFIRLTVANPIKPGTQTSVEKVFQRFYTENTSRTNTESSGIGLYLSKKLVEGM
                     GGEMTARLDGGIFSISVKLRRV"
     gene            complement(17706..18386)
                     /locus_tag="ACG8KR_000733"
     CDS             complement(17706..18386)
                     /locus_tag="ACG8KR_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFQ0200131.1"
                     /translation="MVLLETILIIEDDEAVHSLLEEVLEQRYKLLDAYSGTEGKLLLS
                     TYPVDLILLDLMLPGLSGEALLAEIRQTSNVPIIVLSAKSDQRDKVSLLAAGADDYVT
                     KPFDIEELLLRIGIQLRHQTSVQVKDLSQRIYYKEILLDKESRDVNVNGVAVHLTGRE
                     FDLLKLFLEHPKKVFSRANLYETVWQEPYFDSEKTVNMHISNLRGKLAKAHAVYIHTV
                     WGVGFRFD"
     gene            complement(18387..19169)
                     /locus_tag="ACG8KR_000734"
     CDS             complement(18387..19169)
                     /locus_tag="ACG8KR_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFQ0200132.1"
                     /translation="MFNLLTAEKIKVWKSRKMWVSLGLMVVLPLFYSWNEWYMHTKYG
                     NELNQATDTVINGATGILMVEKMAAWILLAFAAFACFYIGEEFQNGTIRNTLSLGRNR
                     MTYYVSKLLVTLLITTLGVVLITGLAMLAYTLAFGFGEVEGIKNYGNYVLKVFPVLLL
                     LTLGTLSVPVALTFITRSTSVSLLLSFLYIMGTAFIPGVFAKIKGLEFLTEWFTETWL
                     MYTDFAQQATYSQAPKMILVSLVTIVVSSALGMFIFQKSDIK"
     gene            complement(19162..20079)
                     /locus_tag="ACG8KR_000735"
     CDS             complement(19162..20079)
                     /locus_tag="ACG8KR_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFQ0200133.1"
                     /translation="MDFGNETVLKATGITKKYGAAKALDKVSIEIKRGMIYGLIGENG
                     AGKSTFMRTIMGLISIDEGSIELFGTTDLQAARRRMGQSIETPALYPELTARDNLRIQ
                     AANGGVSDREIEDLLKMMRLENTGKKKAKNFSLGMRQRLAIANALITNPEFLILDEPT
                     NGMDPAGMAEMREIIQRLVKERGITVLLSSHLLDELSQIATHYGILHEGHLIKELSKE
                     ELAQESRQFIKIDTSATEQAVTVLDSLGYRDYFVQSSRVIQLFEGIDQVAAINQALVE
                     AKVPVDGIHLVGQKLEDYFLQLTGGNPHV"
     gene            complement(20243..20497)
                     /locus_tag="ACG8KR_000736"
     CDS             complement(20243..20497)
                     /locus_tag="ACG8KR_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0200134.1"
                     /translation="MSTITTITMVASPLGLALFGALFDWKTTQPRLRDSLIFLGGSLA
                     IVILITGLVKLLKLDLKEAKIFSEEEYRETTLVEKKESGN"
     gene            21354..24581
                     /gene="fss3"
                     /locus_tag="ACG8KR_000737"
     CDS             21354..24581
                     /gene="fss3"
                     /locus_tag="ACG8KR_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HFQ0200135.1"
                     /translation="MYKKQKKKLCVLTILSLAVQAIVGAIAPTITFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGFKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSDKAKLVSVLWKKVGTDADTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     EAKINQAIDDYQKKPTFHNSTAKTVLGQSTTVTDTNNLNLSEFDEVVENAANIDYRVN
                     GNQLIITPNANSKESGVLTLKKSAGTGTPVAYKKAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKESGDVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGTKVTIT
                     EKSVPAPYTIDTTPMTTTIKAGETIYVTSKNAREKGQIILDKSGVETGSDLWNDNYSL
                     AGNTFAIRKDSPTGEIVQEMTTDENGHAETPKEIANALELGTYYVTETKASHGFVNTF
                     KPVKVELKYANQTVAPVTSNVKGQNQEVTGETTLTKEDKDTGDKAQGKAVFEGAEYTL
                     FTAKDGKAVKWSEAFKPELAKGTKGSDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYSLDETKYPVSIKKVDDSEKNAVITRNVTAKEQVIRFGFDFFKFAGSAAGTTETGF
                     NDLTFKVSPLEGTNEITGAEDEATTAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKEDFVKSEYVFTITEQDQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLATWKDGNKELTSLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     DATKAAQEKDEKAKPVVIAETSATLANKEKTGTWKIQHKLTAEQVLNKTIVLFNYVYE
                     NKEAFEAGDKPVAKDVSLNNQAQTVSCTVEHHVSIQTKAHLENGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDGTNKEIWKSGKIDYEVNDKEFTKTVLAKKVDT
                     GKYPEGTKFTFAEINYDKDGNINGKHNEDLKEKSQTLTPKEVPTILSTPKQPETPTVP
                     SGSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
     gene            24694..25008
                     /locus_tag="ACG8KR_000738"
     CDS             24694..25008
                     /locus_tag="ACG8KR_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFQ0200136.1"
                     /translation="MELKFVVPDMAETFGKLSYAGEGEIITEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            25021..25395
                     /locus_tag="ACG8KR_000739"
     CDS             25021..25395
                     /locus_tag="ACG8KR_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFQ0200137.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTIGNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIVDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPNQGNPTNQNERKK"
     gene            25405..25740
                     /locus_tag="ACG8KR_000740"
     CDS             25405..25740
                     /locus_tag="ACG8KR_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HFQ0200138.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLVKALTSDEKMFLIIHQGLL
                     GENSDSEEDSDVLDWLGEEESFFIVVEVNENLCRQAESILEEIGVEMPDAIEGFLNQL
                     VETKQLPSS"
     gene            25823..27163
                     /locus_tag="ACG8KR_000741"
     CDS             25823..27163
                     /locus_tag="ACG8KR_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HFQ0200139.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQEFRQFDI
                     SKLSLSTIEIVWSIKDLVCLLGSLAFSGAMILLYIHFFLDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTRETISYFPKICYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDMIANRIGIDEVVAENGTLRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFILDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMARSKAMKEMSNYKTGENYAYLGLPPNFLIFDEYVAYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSELGYS
                     MMFGEVEKNFFMKHIKGRGYVDTGGSVISEFYTPLVPKGYDFLDSISKLEKMND"
     gene            27241..>27786
                     /locus_tag="ACG8KR_000742"
     CDS             27241..>27786
                     /locus_tag="ACG8KR_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HFQ0200140.1"
                     /translation="MESNDSYLEYAKANKDKFISYIIKGKSPEVGRDAVFMAGSPGAG
                     KSEVAIGLAENYDNHVIIDADYFRTQFPDYNGKNSSLFQKASSWLVEQAFRYVLDHGY
                     SFILDATFAILSAEKNISRALKKHFRVTIFYVYQDPKIAWKFTQEREIAEGRHVPKKA
                     FINAFFKSRENIAKVKSKHPDV"
BASE COUNT         8483 a         5466 c         4985 g         8852 t
ORIGIN      
        1 gatataaatg tctcgatacc gaatactcga aaggtttacc agacgataga gcaacaactt
       61 tgtctatctc aatcataaat tcacctggat ctatttttaa tatttttgcg tcaaattcat
      121 tcgcagtatc tcctttgata cgtatgtgcg aagtctttat ttccagaccc agttcatttg
      181 tgatataagc atatacagat ttctttaaat gttttggttc caagcctggt atgactgcta
      241 atggcatgaa tgtttgctca atcgagtatg gttgacaatc aataaaacgg cttcgtacaa
      301 tattatatac atagtctttt acactgatac ttaatttatc agctactttt ttcgatggaa
      361 gttcaattga gaaagtatgg atttctgaat ctacgtgttc ttcaccaacc acgctaaaca
      421 aaccttcgtt tggaccaaga actcgtgagt tatcaattgg tgttctgacg acttcagtcc
      481 catacccact tctacttcgc aaaactcctt cggaaacaag taagtctacg cctttcttta
      541 ctgttaactt gcttgtatga taaataactg ccaattcatc tcctgtagga agtttctcac
      601 caattttata ctctccattt acaatttttt tctttatatc taagtaaatt tccatatact
      661 tcgcaatttt tttcaatggt gaccctccaa tcaaaaacat atcttcatct atactttaac
      721 ataaataaac cgataaaatc cacatcaaaa ttatacacac aataaaaaag atatatatat
      781 ttaaacaaaa ctatttactt taagttgtaa gcggtgttat cattaagtgg aaacaggagg
      841 taaaaaaatt atgacaacta tgaaaaagct aacttttcca gatgattttt ggtggggttc
      901 cgcttggtca gctgagcaag cagaaggaaa aggagaaaca ggaaaagcag agactatctg
      961 ggatcgctgg tttaaagaac aaccttaccg gttttatcaa cgaatcgatt cctctattac
     1021 aaccgatcac atccatcaat ataaagaaga tatagaacta atgaaaaaaa ctgggcataa
     1081 ctcctttcgc gtttctattt cttgggcacg aatgtttcca aatggcggta ccggaaaagt
     1141 aaatcccaaa gccatttctt tctatcgaaa attattttcc gaaatgaata ataacggaat
     1201 caaagtattt gccaacttat atcattttga catgcctgct actcttcaag ataaaggagg
     1261 atgggaatca cgtgaagtag ttgatgctta tgttcattac gctgaaactt gttttaaaga
     1321 atttggtgac ttagtttgtc attggtttac cttcaatgaa ccacttgggc ctattttagg
     1381 tacttatctt gaagattttc attatccaaa cctcattgac tttaaacgcg gagcgcaagc
     1441 agcattcttc actatacttg cacacgcaaa agcaatacaa gtatttaaag aactagctct
     1501 tcctagtaaa ataggcgtta ttctaaattt gagtcctact tttcctcgta gtcaaaatcc
     1561 tgctgatgtg aaagcagcag aaatcgctga tttattttat acgcggagct ttttagatcc
     1621 tatggttaaa gggaattttt ctaaaaagct tatcctgtta ttacaagaat ataatcagct
     1681 tcctgaagat tatacgaaag aagatcttgt atgtatctac gaaaataccg ctcaaattct
     1741 tggactaaac tattacgagc cacgtcgcgt caaatctcgt tcttctatgg tggatacatc
     1801 tagtccattt ttaccagagt ggtttttcga accacatatc atgcctggac gacgaatgaa
     1861 cgaataccgg ggatgggaaa tttacgaaaa aggaatttat gatttgtgca tggatatcaa
     1921 gaaccattat ggaaacatcg aagcatttat ctcggagaac ggaatgggcg ttgcggatga
     1981 agaacgtttc atggatgata cagggcaaat aattgatgat tatcggattg agtatatcaa
     2041 agatcatctt gcttacttat ggaaggcaat aaatgaagga tgtaacatta aggggtatca
     2101 tctttggaca tttattgatt gctggtcatg gatcaacgcc tataaaaacc gatatgggct
     2161 ggtttcactc gaccttacta ctcaaaaaag aacaattaaa aaaagtggcg aattttacaa
     2221 gcaattaagt gatacaaatg gatttagata cgatgtagac ttgttagttg aataatattc
     2281 taaaattcca cttgaagtga acacattaga aaacaagaat aatgagaaat accaataaca
     2341 aatacttttt cttcatactt catttatact tgtcagtata agagactgtg acaaatgtag
     2401 tgatcctcag aagttaggtc tggaaatcta acttgagaag atttttttaa actacgtcca
     2461 gtctttttta tttgtttaaa attgaagttg acaagtcttc attaaaaatt tagaatattc
     2521 acaagtttga cgacaattat gaaaatttat gtcgtgctaa aggagttttg ttattggatg
     2581 aaaagttaga tataaaaact gcgatcaaag acacgttacc taccgttttt ggttatatcg
     2641 gtattggact tgcatttggt atcgttggga aagctgccgg gtttcatccg ctggtcgtca
     2701 cgttgatgtc cctgctgatc tatgctggtt ctgcccaatt tatcacggtc agcatgcttg
     2761 ctagccatag tccactgctc tccatcgttc tctcgacctt tctagtcaat tcccgaatga
     2821 ttctgatgag tatgacgatt gctccttatt tcaagaaaaa tcggctgctt caaaatctgc
     2881 tgattggcac attgctgaca gatgaaagtt tcgcactggg aatgaataaa ttgaactata
     2941 ctggacaaaa gttaaacttt cgttggatga atacagctaa ttggatttct tatttgacgt
     3001 gggtgggttc ttctcttgtt ggcgcacttt tagggaattt tattactgat ccgaaaaagt
     3061 ttggtttgga tttcgccatc gtcgctatgt ttatcgggtt actttatctt caagtcattt
     3121 ctgatagaaa cacctctaaa cgattacagt tgatcctgat tggtctcact ttgattctgg
     3181 tatatgtcgg actgattttt atcccaagta acttagtgat tttagttgta acactgattg
     3241 ggtgcggatt gggggtgtgg atcaaacatg ccttcttttg agtttacatt gctgactatt
     3301 ttaggttgta ctttagcaac ttggctttct aggatcctac catttgtctt actgaaaaaa
     3361 ttcgatttgc cgcaaccgtt gcttgagtat ttaagttttg tcccgatcgt cattatgtct
     3421 gctttatggt tcagcagtct atttacccaa aatatcggac atctgccgca gatcaatctc
     3481 gaaaatgctt tggcttcagt tcctacactt ttagctgcta ttctttcaaa aagcttattg
     3541 gtgatcgtct tggtaggaat tctttccctt tcgttgattc gcttattata aataaaaaaa
     3601 caagagctta tggcaaatta ctgtcatgcg ctcttgtttt tatcaaagtc aaataagaaa
     3661 aaaatcggcg tattcttgct tatccgttga acggaccact aacaacgact tttcctaata
     3721 tatgtcctgt ttctacgtct ttggttgctt gtttcaagtt cttcgcattc atcccatctg
     3781 aatagacctt gctcaatgta ctgcggataa tcccttcatt tgctaaagca gcaatgtgcg
     3841 ctaaagcttc tccttgcgtt tcgatttcat agttatagtc tgttttagcg aacatatact
     3901 cccagtcgaa gctgatagat tggtttttcc aaacacctat atctaacggc tctttgatcc
     3961 ctacgatcgt tcccacatgt ccaaatggtc ggacaaggtg tttgatttga tcaaagtagc
     4021 gggtaatatc gaataataca gcgacatact ctacttgatc gattcctaat tcacgaaaac
     4081 ttttacttaa gtcgtgatga taatcgattg gatagtctac acctgttttt ttcagccatt
     4141 caaaattctt cggacttgca gtcgcgtaga cttctaggcc agcccaatgc gctagttgat
     4201 tcaaaatcga accgacgccc ccagctccgt tgatcactaa gatttttttc ccgcggttgg
     4261 cattttcttc tggagtcaga ccaaattttt caaataacaa ttcataagcg gtcaatgagg
     4321 tcaacggcaa ggctgcggct tcttcatctg aaagattttt tggtgcaaga gcgacaatac
     4381 gttcgtcaac cagttggtat tcttgattgc ttcctgctct tgtcgttgta cctgcgtaaa
     4441 aaacgcgatc gccgacagaa aatttttgca cttcatcacc tacagcaaca actttcccga
     4501 ctccatcaaa ccctaagata cgtaaagcat ttcttatccc gttatcttgg cgaagtttcg
     4561 tatcgacagg attaacagat actgcgttga cttttaccag aagatcccgt gaactgggta
     4621 caggtatctg ttctttttca tcaataaagc tttttggatc atcgatcgac aacccttcat
     4681 aaaaaccgac taccttcatt aattcctttt ctgacatacg aattcctcct tagcttcatc
     4741 aagttggagc atccatacag ttttcatttc aatatatata gtatgctatt tgttccactt
     4801 ttattcatca taattattct cttacttaaa gtaaactatt tgcacttttt tggttaaaaa
     4861 agaggttgcg acaagacttt tgtcacaacc tcttttaaac ggtgttcaat caactgaccc
     4921 tcggtattcg ctcaaaaaac gacaaaatat gaggagctat ttttgtttgg ctgttcttat
     4981 tacttgatgc aggacagctt tttcacgacc tctaattcac ggtgttcaag caagaaatca
     5041 ttgttcactg aaaagcgttg gatgttcttt ttcaaaggtg tctaagatct ctcgtacttc
     5101 atcagtagat ttcgtattca tcaactgaac tcgcaagtca tttgctcctg gaaatccttt
     5161 gatatagatt ttaaagaaac gatgtagtcc tgtgatggaa cgcgggatga tttctgaata
     5221 atgatcttgc agatctagtt gcagacgaag tagtccgatc agttcttttt ctgtatgttc
     5281 cctcggttct ttttcaaatg cataaggatt cttgaaaatc ccccggccaa tcatgatgcc
     5341 atctacgcca tatttttcgg ccaattcttc tcctgtctgg cgatctggga tatctccgtt
     5401 gatcgtgatc agtgtttgcg gagcgatttg gtcacgtaaa gcaatgattt tagggatgac
     5461 ttcccaatgt gcatctactt tactcatctc tttacgcgta cgcaaatgga cagaaagatt
     5521 ggctatatct tgatgaagta ggtgactgat ccattcttcc atctcagaca tttccgtata
     5581 gccgatacgc gtcttgacgc tgacaggcaa accgccagcc ttagccgctt caatcaattg
     5641 tgccgctact tctgggcgaa ggatcaagcc actgcctttt ccacgttctg ctacattagg
     5701 gactgggcag cccatgttga tatcgattcc ttggaagccc atttctgcca tagcaatgct
     5761 catttctcta aaaaattctg gcttgtctcc ccaaatatgg gcaaccatcg gctgttcatc
     5821 ttctgtaaag gtcaatcttc ctcgtacact gtcttttcca tctggatgac agaaactgtc
     5881 tgagtttgta aactcagtaa agaagacatc cggtgctgct gcatgcttta cgacatggcg
     5941 aaatacaaca tctgttacat cttccattgg tgctaaaaca aaaaaaggct ttggcagctc
     6001 tttccaaaaa ttagtagtca tttatttgtt actctccatt ctatcaagca gtgaagtcgc
     6061 aatctttctc aaaaacattg tcattatact aggaaacaaa aaaagaagca aagtgaacag
     6121 cttaaaagat gtttctgatt gagcaaaaaa agactgtgac aaaaatctta tcacaattcc
     6181 ttttccggaa taaatggtgt tcaaaagctt gcatctgcgg taataagtcc aaccaaacga
     6241 aaaataggag gagctatttt tgtttggctg ttcttattac ttgatgcaga acagcttttt
     6301 cacaacctct ccactaaacg gtgttcaatc agctgaccct cggtattagc tcaacaaacg
     6361 acaaaatatg aggaactatt ttcccgtttg tttctctaat actcaggtca aaacgctgat
     6421 ttcacaacct cttttctaac ttctcaatcc tcttccttta ttgatcaaat accaagtaac
     6481 ggcgtacaaa acaacgatga atagcacaag tacgatcatc gatgttgcta ctggcacatc
     6541 gattgttcca aggaatccgt aacggaaacc ggagatcata taaacgatcg gattgacttt
     6601 cgaaacagct tgccaaaatg gcggcaacat agagatggca tagaaaacgc cacctagata
     6661 agttaacggc tgcaagacaa atgtaggcac gatcgataca tcgtcataag actgagcaaa
     6721 aatcccgttg agcagtcctg ctaacgaaaa caagattgcc gtcatcaata atgtaatgat
     6781 gaccatgaac catgaataca catgaagtgg tacaaagaac aaggaaatga tcgttacgag
     6841 agagccgacg agaatactgc gacccactcc gccaatcaca aagccccaaa tgatcacatg
     6901 cgtaggaaca ggcgctacca agatttcttc gatgtttttc tggaattttt gagagaagaa
     6961 ggacgaagaa acgttggcat acgaactagt gatagcagac atcataatca aaccaggcac
     7021 gatgaattcc atatatgaaa aaccacccat gtcaccgatc cgaccaccga tcatttttcc
     7081 gaaaataaca aagtacaagg atgtcgtgat cacaggcgga accagtgttt ggacccagat
     7141 acgcaaatag cggttcgttt ccttgatagc taaacttttt aatgcagtaa aataaagatt
     7201 aaacattttt ctcctccgta tgattatttt cttctgtgat tttcaaaaac agttcttcta
     7261 aacgattcga cttattcctc atagaaagaa ctttgattcc ttgtcttgtc agctgatcga
     7321 agattccgtt caccccttga tttcgttcaa cttcgacagc gattgtctgt tcatcttcaa
     7381 atgtacaagc gtatccttca atcacaggtt tggtctcata tggcgctaaa tcaaagataa
     7441 atgtttcaaa ttgcagtttg gatagaagct ctttcatact agtattctcg atcagttcac
     7501 ctgattggat gatcccgatg ttgcgacaaa gcatttcagc ttcttctaaa taatgcgtcg
     7561 tcaaaatgat cgttgtacca ttttcgttca attctcggag aaactcccac atctcgcgtc
     7621 tcaattcaat atcgactcct gctgttggtt catccaagat cagcaaacgt ggttcatgca
     7681 tcaatgccct agcaatcatc agtcttcgct tcattccccc ggaaagcata cgtgcacgga
     7741 catttctctt ttcccaaaga ttggactgct tcaaatattt ttcgctgcgt ttcatcgctt
     7801 ctttgcgaga gacaccgtag tagcctgctt gattcacgac gatttgctgg accgtttcaa
     7861 agggattgaa attgaactct tgcgggacta atccaatctg ttgctttgct cggactagat
     7921 ccgtatccag atcataaccg aagacattca cttttccaga tgttttattg actaaagaag
     7981 taatgattcc gatcgttgta gacttcccag ctccatttgg tcctaatagc gcatagaaat
     8041 ctccttcttt cacggaaaga tcgattcctc gcaatgcttc gaccccactt ccataaacct
     8101 ttttcaaatt atttatttca agcgcatttg ccatttattt cctatctcct tatagacatg
     8161 attctttatt gtatcatgga atacagcaga tgaaaaaaca acttggttcc agcgaaaaat
     8221 caggttgctg ggctcaggaa aatctcctaa tgaaagacat caatcggacg aagtcagaca
     8281 accttttcag aaaaaacaaa tcgaagataa aggattacta ttcatatgtg caacaaaagg
     8341 agcgtgagac aaaagtaaag aacacttttg tctcacgctc taaacacgta taaacggcgg
     8401 aagcaaaagc caagattttt gatccaataa atactttcga tcacttcgcc tatcccaata
     8461 catgccactg ccgcttttca tcaaataacg cggcttccgt ttttgcggtt tctactaaag
     8521 tctctgggta atccaaaact ttcagcaagg caatcgcatt tcgggtcctt gaaggtcctt
     8581 cttttaattt gaaatcaaaa ctgattcctt ttccttctgt tacttgttct tcaaagtgga
     8641 cattctcaca ataattcttt aaaatctctg tcaattcgat atcatgtgtg gcgacaaatg
     8701 ctaaagatgg atactccgca agccaccgaa cgactgaagc ggaagaagca atccgttcga
     8761 tcgtatttgt cccttttaaa atctcatcta cgaagcagta gcagcgttct ttggaagcca
     8821 cttggtccaa caaacgtttg atcgatttga tttctgaaac aaaataactg tcgccttcaa
     8881 ataaatcatc ttcgattgcc atggaagtca acacatgtcc aggttgcagg gtaaactgtt
     8941 ctgcaatcgc tgtttggatc gtctgtgcta gaatacagct gatggctata cttttgacat
     9001 acgtagattt tcctgatgca ttggaacccg tgactaatgt attcttctgc cagttgaccg
     9061 gattcactac cgcttctttt aacaatggat gatagatatc tttcgctgtc acaccgccct
     9121 cagtaaactc tggtcggcaa gtgatgggca tataagtacg gtaattcaat attgcagcgg
     9181 ctacttctaa ttctcccagc aattcccaca gcttgatggc ttcagttgaa tgcttttcta
     9241 atttagtgag aacgaaatga taggagataa atggcagcat gaacatgatg ttgacatatt
     9301 caaaaatcaa ctctgcttca ctcccactct tcatacgaaa tgaaaaacct aatttaggga
     9361 tgtctcttaa tggtttagtt tgctgtttca gctcttcttg aagtggtgtc ggcagttttg
     9421 ctagctgatt ggcggctgaa atcgttgaaa ccaaataact catgctattc agctctgttt
     9481 ctagtgtttg tttcttcact agataataga ccgtattgaa tactaaccca ccgattgcca
     9541 aatagattcc tagtacttgt cctaaaagaa gcaaacccaa acccaccagc ggaagcaagc
     9601 caagaaaaat aaataaacct acgcgtccaa tctttttact ttttccatcc gctagatatt
     9661 gttttgcaaa attatgatct ttttttccta aacgagcgaa ttgatattgg attttttctc
     9721 tcagttgagg attttcctga taaaaagcaa tcagtttttc taactgctga tcttcaccaa
     9781 aatcaaagct gcgtaaacgt tgatacaacg cttctgaacc tacactagaa taggtactgt
     9841 tgattccttc gaatacttcg aacatatcca ggtcatacca agtaagatca tcgatttcgc
     9901 tatcccatga acggaacttc ttctccgtga gccatgcttc cttcaagctt tcttctttat
     9961 caaatctcgg ttgataagga attttttccc atttgctacg aactgtttcc tttaatttca
    10021 aacgtgaaaa aatgtctaaa gcaactaata aaataacagc tgcaataata attaaaacga
    10081 taaattctct ttgacccata tccctctccg ttcctttatt tgttagcttt agtatagcac
    10141 tgttgcgcta tttgaaaaga gggtaaaaaa acacctttca aaataaaagg tgtttttttc
    10201 ttgtatatta catatttatc tctcatttat caaaattgac aaataatcat agggaggaat
    10261 tgttgttaat tcttgtttat tctccagtgc attgacaact gtcccaaggg aaaggatagc
    10321 ctcaggtact attttttctt gttctgttgg attagctaag cgaagaacaa atccttgttc
    10381 atgaaaataa gaaggataaa tcgatgatac aattaagtct tcaggtaatt caatcaatga
    10441 aaatttacga ggaacatcaa gttcattttg taatggccaa atcttgttat ctaacctgtg
    10501 aataaataca ttcagtttct gcttttgata agaaatagat tgttctaacc gtttttctgc
    10561 ctgttttgcc actttgaact cattaaatct tccttcttct acattaaatc caaaagtgac
    10621 tttatactca cctatctctt gagctagcgg tgtttccatc atgacatgcc cttcatttgt
    10681 cgtatctcct gaagctcgcc caggtcgcca agcaagatta ggttttccaa gttcccctgt
    10741 tgttgaaaat aaggttatga ttaattgatt ttctatacgt tcatactctt ttaaaccatc
    10801 tgcataaaac gtaaaataat gatcattttc tgccacagag acactcttat cgaatatttc
    10861 taaattaaca ggtttctcta ccatagtttc tgaccaattc ggaggaatga catcaggggt
    10921 attttcaata aatccgtttt gaatttgtgc aattgatttt tcagatttat ctaaaacatt
    10981 aattgctaag cgcatacgat gcgagtaaac agtattatga attttgagtg tgccttcaac
    11041 gatttgactt tctcccagac tgaacgatat ttcataagat atttcttcca tttcgggttt
    11101 ttcagataaa cgatctttta aatctttagg aagttgagct tttccgtaaa caactaatgt
    11161 ttcttgtaaa gaatctttat atactttagc tgtttctaat tttagagata gttctgtatc
    11221 tccttctaat ggtgaataat catacgtatc gccatcattt gctgaatcga tcaaatgaac
    11281 gaaatcaaga tattgtctat ctccaactat caaattcaat tgcccatctt taaaagaaag
    11341 tttatattta ttgttactta tctcagtttt gtctatctcg attgctgttt ctaatttttc
    11401 attatttttc tcaaattcaa caacagtgta tcctagtgct ggcacattta catttatttt
    11461 tatgtcaagt tcataataag ctggttcatc aaaatattcg aaacctgtag caatcatccg
    11521 ataagcattt ttccgttctg gaaaataaac ttctttttca atgatattat agggactacc
    11581 tttgaagtgt attttctttg aatcagctac tacatgcacg attttcgatc cactataatt
    11641 ctcagtcgtt gtgttgaaaa caaggacttc attcgatttt agctttaatg catcagccat
    11701 ccgttttaca ataaggtttt cgattccgtc agctatctca ttagcttctt tgattcgatg
    11761 gaaaatatct tccgcaacat tatctgaaac acagccacca atactatcat gagcctggtt
    11821 ttgcaaaatc tttttccata accgacgaag taggccttta ctgatatgga taccatttct
    11881 ttctgcaatc agcatgagcg gctcaactcg ttttagaatt ttttgttcca atttaaaatt
    11941 ttctattttc atttttgttc ttactgagcc aatagaacga tgaacacgag agtaaaccgg
    12001 ctgacgaagt tcacctttat attcaggtaa ttcttttttg tttctgataa cttctacata
    12061 agaaggataa tctgaaataa tattgtggaa tttactgtat ttattcaact tctctacgac
    12121 ttgatcaaaa tttaaaatca ttgacttttg atctacaccc gaaggtacta aaatatcttt
    12181 gttttttcct tgcatatgaa gcatttcaac tgcttcatcc aacttttctt ttgcaaattc
    12241 aggaatatct ttaattgcgt ctaaggtcat cattccagtc atataaccaa atggaaaatt
    12301 catggcataa acaaatttat ctcccaatcc tttccagata aaataaggag atggaaccat
    12361 cgtttcaaag tttaatcctc gccaacacat gaaattatcg atttctactt gatgcaaaag
    12421 tgtaggcaaa ttcgcattga acccaaacgt gtcaggcaaa taccctacca tcattggatc
    12481 tccatattta aggatcgtat cactgattcc aatcattaag tttcttaaaa ttgattcagc
    12541 atccactaat aaggcatctg tttgtgcata ccacggtccc acgaatagtt gtcctgtttt
    12601 catcagtcgt tggataacct taagcttttg cggattgatc tccaaatact catcgacgat
    12661 tgaagtttgt ccatctaaac aaaagtttac tttagggttc ctttccaatt catctagaac
    12721 ttcagtaaag acctgatcag acaatacgat ggcatcttca tttgtaaaat accattctct
    12781 gtcccagtgc gtgtgttgaa ttatatgtgc atttaccata tcagattctc ctactttcct
    12841 tcttctactg aatttacttg ttgttctttt ttatacttga tggattttag tgcattgacc
    12901 attaacgctg taaccactcc cccaataaca attgatacta aatataaagg tatattcgta
    12961 acaaaaggaa ttgccataaa ggttgaaagc attgtttctt gagtaacacc aaaaaatcca
    13021 tttagaccac atgctactgc agtgcccacc acgatgcttg gtattactcg taaaggatct
    13081 ttcacggcaa acgggatcgt cccttcagta atccccacaa ggcccataat catagctgtc
    13141 tttgcattat ctcgttccga ttcatcataa agttttttgt ttaaaattgt tgctaaaaac
    13201 attccaaatg gcggaatagc aattcctaat aacatggctg tactaggtcc aaagcttccg
    13261 cttgaaaaag aagcaacccc aaaagcatat gcgattttat tgataggtcc tcccatatca
    13321 aacgccatca ttcctcctaa aatagccgac atcaaagcag catttgctcc tgacatgcct
    13381 tctagccaat tagtcagtcc tgttgtcatt gcttgaatag gcgtagctag tacataccat
    13441 aatatgattc caacaactaa tgtagtaaca aaaggaatca gcataattga ttttagacta
    13501 ataacttgca tcggtacttt tacttttttc aatgcattag ctaaatagcc acaagctaat
    13561 ccagcaacca ttcctccaat aaatcccata ccaagatttt gacacatcat cccagtgata
    13621 aaagctggtg ctagaccggg acggtcagca attgaatatg cgatataggc tgaaatcatc
    13681 ggtacgataa actgtaatcc cgtctgacca atcattcgca ggactcccca gaatgtatcc
    13741 gtctggtccc aaacacctgt gccaccaaaa atgactgcaa tagaagttag taaccctgct
    13801 gctacgacaa gcgggatcat ataggaaacc ccactttgga aatgatttag aatttctctt
    13861 ccttgtttct tgaattccat tagtaactcc cccacgatat attttttatt tgttaattaa
    13921 tgcaattgct tcatcaatcg tctcttcacc atcttttact acctctgagg tccctactgt
    13981 taaaatcggt ttaccgtcaa agcgacctcg ttctgaaaca tttgtatcta ctgcaaatat
    14041 gacaacatct gcctcggaaa catccgaaat tgaaagtttg ttttctatcc ccatagcccc
    14101 ctgtgtttct actttaatat ctaacccttt cttcttcgca aacttttcta aattagcttt
    14161 tgccatataa gtatgggcaa tccctgcgat acatgctgtt actgctacta tttttgtcat
    14221 atgaaattcc tccttttaaa atgatagagc ttttaccaat tcttttttat ttctcgaatc
    14281 ttttattctt ttaacaaact cgtcatccat caatcttcca gataattctg ctaaaatacg
    14341 taaatgatta gtacctttat cagtgtcttt tattgctaac aaaaagacga gatcaactgg
    14401 ttgatcatct aatgcgcccc attcaacaga ccgctttaac ttggctacag ccactgttga
    14461 ttcaattact gctaggcttt tgccatgagg aatggcgata tttttcccaa ttcctgtggt
    14521 catatggttt tctctgtcaa atacgtcttt ttgaaattgt ttgtggtcag acacaaaccc
    14581 attagtctct aacaatttcg ataatttttc gactacactt tttttatcag atgcatttaa
    14641 atctatcact atattattta tattgatttt ttcactgatc atttataagc cttctttcaa
    14701 tatataaact aatttttcaa acgacgaact cgtttttaaa tttgctaata actcatgatt
    14761 cgaagcgatt tcattgaatg tttcataaat tttattcaat tcaggtttta ctttttcatt
    14821 aagtgcaaaa aagaaaacag aatgaacaag tgtcccatcc caatcaattc cttctggatt
    14881 aacgtagaca cagattactg gcttttttat aaaatctact tcgatatgcg gcatagcaaa
    14941 atcattcatc gctgttgaag atagtttttc tcttttgatt gctccctcta gtatcccttc
    15001 tttcgcgtaa ttttctttta ccaatttttt ggtaattata tttaaaacgt tcacttgatt
    15061 atcctttatt tggtgggtaa aaaagcaaaa tcgtttatct aataaattta gaaaagcatc
    15121 ttctgtttgt tcatttaact gtagatactg ttttactttt ctgacatctg cccctaacat
    15181 caaaggacta acaacaataa caggaaaatt tgtattttca atcggaatcg tgcttataac
    15241 aagttcatca gtgacttttc tatcttgtag ttcattgatg cttagtacat cttctatctc
    15301 aaccatatct gcaaaaccgt tatttattct ttgctctaat aattttgatg ttccatatcc
    15361 gctactacaa actaatatca ctttcgtttt gtcaggtttg taacgatcca aaagtgattg
    15421 aatatggagt gttacgtaag cgatttcatc ttcattaaaa tgtatacaga atctctcttc
    15481 gatttcctct aataaatcta cacttatcat aaatgacctt ttaaaagaat atttgatttt
    15541 gtccgtataa gggttataga tatttactcc caatttgagt cttttaactg ctgcattgag
    15601 atgtaatact agattattga tcaattcttc atcaggataa aattcattta atttaattct
    15661 tactaactct tgtagctcaa cacccatatc taaataggaa agcttcgttt gatctaagtc
    15721 gtgattcata gcattttttt caatcgaagt aatgtgaatg tcaacataag cttgttcatc
    15781 tattggcaca ataattgaaa atgctttttc aatcattttg attaaaataa gtgtattctt
    15841 tgagatttct tcttcagaat tattactacg tgataagaaa ttgcttgttt caatcctttc
    15901 tatcgcaata gcaaggtgga tagctaaagt ttgaaattca taatctgtaa atttcaattg
    15961 tgtcttattc atgtatcgat taagcaattc ggctactttt ttcaatatgt ccttatttaa
    16021 gaaattattc gcttcaccaa tgaaattgat attcaagtct aagagatctg ttttactgtt
    16081 agatgctgta aagccacccc aataatagtt cacaacttct gacatcaacc ttcttttact
    16141 tttttcatca gctttgatgc gcacgccatt tcgatctgaa actaaggaca tcccatgctt
    16201 gtttagtctt tttctaacat ctttcattgt gctttctacc gtcgacctgc tcacgtataa
    16261 ttcgtcagca agattttgaa tctttagaga atcattagta ttcaataatt tagcaaacac
    16321 ataagttaga cggtcctccg gagagtctgc gatattgatt ttatgactgg ataataaatt
    16381 tcgaagtgtt tctttttttc caataagcga aacgcctttt ttaggttgaa ccaacatttg
    16441 tacatctggc attttttgga ttatttcttt caaaattaaa atatctttgc taattgtttt
    16501 cgtagataca tataattgtt ctgataattc tttgtaagtt gtgtgctcat tttcaatcaa
    16561 aaagtatagt attttcattt gccgttcgct aaattcgatg cccattatct tcacctcctg
    16621 aatacagctt aagtgtacaa ttattatctc tattttcata cagaagaaag ttcttgatta
    16681 atgtagaagt ttctaatatt cttgattatt tcaaatcaat aaaaaagcct gcaatattta
    16741 tatcacaggc ttttttctct attaaatata tataagataa tacatttttc tactagttct
    16801 cactaatctt gtataattaa accctccgca acttgacgga gatggagaag atgcccccgt
    16861 ctaatctagc ggtcatctct cctcccattc cttccaccaa tttttttgac aggtaaagcc
    16921 cgattccgct agattccgta tttgtgcggc tggtattttc ggtataaaaa cgctggaaga
    16981 ctttttcaac agaggtttgc gttcctggtt tgataggatt ggccactgtt aatcgaatga
    17041 atggatcctc ttttgaaaat gtcacctttg cttgtccatc tgcataacgc aataaattct
    17101 gtatgatatt ttgcagcact cgcagcaaca tgagcggatc tgctaataca gttgtattta
    17161 tcgagtgttc agcgaacgct acctcgatat ttttctcaga aaattgttca taaaaagaga
    17221 aaaatacttc ttccacctct ttagaaaaag aaagcgtatg gagatccatt tctttttgct
    17281 tcgtctctat tcgagcaaga tcataaaact gatccgtcat actgagtaat cttgcaacag
    17341 attgtttgat gatcgttaaa tacttctccc gtttctccgt atcctctacc tgttccagta
    17401 attgtacata accgttaata gcagtcaaag gagttctgag atcatgagaa aggccgacga
    17461 tcgatagtct gatgttttct tccatttctt gaaatgcttg tcctttttgt tcattttcat
    17521 ccaaccatgt attcagtgca tctactaggt ccattagatt cttttctcga aattcgactg
    17581 aaagacgacc accatattct tgtcgttcag aaagagtggc gattttctta gttaatctct
    17641 tgatttccct ttgcaggaag aaaataaaac ttcccataaa gataacaacg agaagcaaca
    17701 gccatttaat caaatctaaa ccccactccc cacaccgtat gaatatacac agcatgtgcc
    17761 tttgctagct tccctcgaag attggaaata tgcatattca ctgttttctc actatcaaaa
    17821 tatggttctt gccaaactgt ttcataaaga tttgctcgcg aaaatacttt tttcggatgt
    17881 tctaaaaaaa gcttcagcag atcgaattct cttcctgtca aatggacagc cacaccattc
    17941 acatttacat ccctcgactc tttgtccagt aagatttcct tgtaatagat gcgttgcgac
    18001 agatctttta cttggacaga agtctgatga cgaagttgga tgccgattcg taacaaaagt
    18061 tcttcgatat caaatggttt cgtcacatag tcatccgctc ctgctgccaa caaggaaact
    18121 ttatctcttt gatcgctttt tgctgaaaga acgatgatcg gaacattcga tgtctgtcgg
    18181 atttctgcta acaatgcttc acctgaaagc cctggaagca tgagatctag aaggatcaaa
    18241 tcgacaggat atgtgcttaa caataatttt ccttccgtac ctgaataagc atcaagaagt
    18301 ttgtatctct gttcaagaac ctcctccagt aaggaatgaa cagcttcatc gtcttcgata
    18361 atcaaaatag tttccagcag gaccacctac ttaatgtcag atttttggaa aataaacatt
    18421 cctagagctg atgataccac gattgttact agactgacca gaatcatttt cggtgcttgc
    18481 gaataagttg cttgttgcgc aaagtccgta tacatgagcc acgtttccgt aaaccactcc
    18541 gttagaaatt ccaagccttt gatttttgcg aaaacaccgg gaatgaaggc cgttcccatg
    18601 atataaagaa aagaaagaag caaactgaca ctggtacttc tagtgatgaa tgtcaaagcc
    18661 actggaacag ataatgtccc taatgtcagc agaagcaata caggaaatac ttttagaaca
    18721 taatttccat agtttttgat tccctcaact tcaccaaatc cgaaagcaag tgtgtaagca
    18781 agcatggcta gtcccgtgat caaaaccacg ccaagtgttg tgatcaaaag cgtaacgaga
    18841 agtttcgaca cataataagt cattcgattc cgtcctaaag ataacgtatt gcgaatcgta
    18901 ccgttttgaa attcttcccc aatataaaag cacgcaaatg ctgcgaaagc cagaagaatc
    18961 catgcagcca ttttttctac cattaaaatc cctgttgcac cgttgatgac tgtatccgtt
    19021 gcttggttta attcattgcc gtattttgta tgcatatacc attcgttcca agaataaaaa
    19081 agcggaagta cgaccatcag tcctagtgag acccacattt tacggctctt ccatactttt
    19141 attttttcag cagttaatag attaaacatg tggattgcct ccagttaatt gtaagaaata
    19201 atcttccagt ttttgcccaa ctaggtgaat accatccaca ggaacttttg cttctaccaa
    19261 tgcttgattg atggctgcta cttggtcaat tccttcaaaa agctggataa cacggctaga
    19321 ttgtacaaaa taatccctat aaccaagaga gtcgagaacc gttactgctt gttccgtcgc
    19381 cgaagtgtca atttttataa attgacgaga ttcttgtgcc aattcttctt tcgataattc
    19441 tttgatcaag tgtccttcat gcaaaatacc atagtgagtg gcgatctgtg acaattcatc
    19501 tagcaagtgg ctagaaagca agacggtgat gccccgttct tttaccaaac gttggatgat
    19561 ttctcgcatt tctgccatcc ctgctggatc catcccattc gtcggttcat ccaaaatcaa
    19621 aaattctgga ttcgtgatca gtgcatttgc aatagctaaa cgctggcgca tacctaaaga
    19681 gaaattcttt gctttctttt tccctgtatt ttccaatcgc atcattttca gcaggtcttc
    19741 gatttctcta tccgacacac cgccgttcgc tgcttggata cgtaggttat ctcttgcagt
    19801 taattctgga taaagagcag gggtttcaat cgattgaccc atacgtcgtc ttgctgcctg
    19861 caaatccgtc gttccaaata attcgatcga accttcatca atcgagatca aacccataat
    19921 agttctcata aaggtagact tacccgctcc attttcccca atcaatccat agatcattcc
    19981 acgtttgatt tcgattgata ctttatctaa tgctttggca gcaccgtatt ttttggttat
    20041 accagttgct ttcaagaccg tttcatttcc aaaatccata cttatccttc tttcttattg
    20101 atgagtgaag tatagttgac gggtataaag aaagcagcca gaaatgtata aagaaagtgt
    20161 aaattttcca attgaatgtc aaaaaaaaca atcggacaat acgtgtcttt cctattatca
    20221 ggcagtttgt tatacgcttg tatcagtttc ctgattcttt cttttcgacc aaagtcgttt
    20281 ctctgtattc ctcttcactg aaaatttttg cttctttcag atcaagcttg agtaatttta
    20341 ccagaccggt tataaggata actatagcaa gactccctcc cagaaaaatt agactgtctc
    20401 gcagacgtgg ttgcgttgtc ttccaatcaa agagcgcacc aaacagtgct aatccaagtg
    20461 gactggccac catggtaatc gttgttatcg ttgacataag tcgtccttgt atatgcgttg
    20521 gaacttctcg ctgaagccag accatataaa aagcaaaaga aaacattaat ccttaaaaac
    20581 ggacttatga aaaataatac atgccgtttg ggatttacaa ttagctacca aataagaggt
    20641 tatgacaaaa gacttttcac aatcccttct tccgaataaa cggtgttctc ctgccaactc
    20701 ctcggtcaca agtcacaata tttatcaaac atgagaagca atttttgtaa attccttctt
    20761 atttcttgaa gctgaccact tctgtcacaa cctcagaagc ggataaataa tattgtttga
    20821 tgatacttta tctttttccc gtttgtttat agatcgaatg atattataga ccaagaatcc
    20881 tacctattaa catagctaaa aacacaaaaa aggcactttc caaattgtgg aaagtgccag
    20941 aaaagttagg attaaggtaa ctgcgctcag cattgcttcg cccagtacga ttctcgtaac
    21001 tcgtgaactc gaacgataat cgcaattaac gttttgagaa ctgtgaagct ttacgggctt
    21061 tcttaagacc tggttttttc ttcgtatcat tgaataagtt tgaaaaacct tgatttataa
    21121 aggttttacc gtaaatatat tttattatat ttgattaaat ttgaaatgtt tgtgttctaa
    21181 aatgtgttct aatttaaagg cagtatattt cgtattttag attaaatttt taggctcatt
    21241 cattctcaaa tccattctat tcagtcaata aaaattttaa aaagacttta taaacaaaga
    21301 tgttcaagcg agcatcttat ttttttgtgc ttaatttgaa aggagaacat ttcatgtata
    21361 aaaaacaaaa aaagaaatta tgtgtattaa ctattttgag tttggctgtt caagcaattg
    21421 ttggagcaat tgctcctacg attacttttg ctgatgaaat tacccatcca caaacggtaa
    21481 ccgtagaact agatttagca catcaatatg ctgttgaagg aacttttagt gatggtcgtc
    21541 caatgtcaga agtcactgtt ccgcattatg cagtgtacaa cggctttaaa caagatattt
    21601 tctgtattga accaggagtc ccgatttaca atgagttcac tcctggctat gagaaaaacc
    21661 cgttaccaga tatgtccgat aaagccaaat tagtttccgt tttatggaaa aaagtaggta
    21721 cagatgcaga tactcatatt gtggcacaga agatgatctg gcaagaagtt aatgggtata
    21781 cccttcactc aataaaacga tcagatggta gtgccgtaaa tatcgcagcc attgaggcaa
    21841 aaataaatca agccattgac gattatcaga agaaaccaac cttccataat agtacggcta
    21901 aaacagtatt agggcaatcg actactgtga cggatacgaa taatctgaat ttgtcagagt
    21961 tcgatgaggt tgtggaaaat gccgcaaaca ttgattatcg tgtgaatggt aatcagttga
    22021 taatcacccc aaatgccaat tcaaaagaaa gtggtgtgct gacgcttaag aaatctgctg
    22081 gtaccggaac acctgtcgcc tataaaaagg ctggccaaca gactttaatg gccggggcga
    22141 ttgataaacc taatacctac acaatcaaaa ttgatgtgga aacggagggt tctttaaaga
    22201 tcaaaaaagt cgataaagaa tcgggtgatg ttgtaccagg aacggttttc catttagact
    22261 ttggaaaaac tttacctgca aaagacgtga ctactgacaa agaaggcatt gctacattgg
    22321 atgagattcc tcatggtaca aaagtaacca ttaccgaaaa atcagtaccg gcgccttata
    22381 cgattgatac cacccccatg actaccacca ttaaagcagg cgaaaccatt tacgtgactt
    22441 caaaaaacgc acgggaaaaa gggcaaatca ttctggataa aagcggagtt gaaacaggga
    22501 gtgatctttg gaatgacaat tattccttag ctggtaatac gtttgccatt cgcaaagaca
    22561 gtccaaccgg tgaaatcgtt caagaaatga ccacggatga aaacggtcat gcggaaacac
    22621 caaaagagat tgctaatgcg ttggaattgg gaacctatta tgtgaccgaa actaaagcta
    22681 gtcatgggtt cgtgaatacg ttcaaacctg taaaagtcga attgaagtat gccaatcaaa
    22741 ccgtagcccc tgttaccagt aacgtaaaag ggcaaaatca agaagtcact ggcgaaacca
    22801 ctttgacgaa agaagacaaa gatactgggg ataaagcaca aggtaaggca gtctttgagg
    22861 gtgctgaata caccttgttt actgctaagg atggaaaagc tgttaaatgg agtgaggctt
    22921 ttaaaccaga gttagcgaag ggaacgaaag gttctgatga aacggtcact ttgactttag
    22981 acgaaaagaa ccaagttgcc gttaaacatt tagctatcaa cgagtattat tggcaagaaa
    23041 caaaagcgcc cgaaggatat agccttgatg aaacaaagta tcctgtatcc atcaaaaagg
    23101 tggatgatag cgaaaaaaat gccgtgatta cccgaaatgt tacggcaaaa gaacaagtga
    23161 ttcgctttgg ctttgatttc tttaaatttg ctggatcagc tgcgggcact accgaaactg
    23221 gcttcaatga tctgaccttt aaagtgtcgc cattggaagg gaccaatgaa atcacaggtg
    23281 ccgaagatga agcgaccaca gcttataacg agcaattagg gtttgatggt tatggcaagt
    23341 ttgaaaatct tccttatggg gattatttac ttgaagaagt agaagcccca gaaggttttc
    23401 aaaagattac gccattagaa atccgttcta cttttaagga aaacaaagaa gactttgtga
    23461 agagtgagta tgtctttacc attaccgaac aagaccaaaa acaaccaatt aaaacggtga
    23521 ccgttcctta cgagaagctg acgaacaaag cattttctgt tagtttgaac cgtttaatgc
    23581 tctatgattt gcctgaggaa gaagatagtt taacttctct tgcgacttgg aaagacggaa
    23641 acaaagaatt aacttctctg gattctaccg agctagttga taaattgagt tacaatctac
    23701 atgaaatcaa agaagactgg tatgtggtag cgcaagccat tgatgtggat gcgacaaaag
    23761 ctgcccaaga aaaagatgaa aaagccaaac cagtggtgat tgcagaaaca agcgcaacgt
    23821 tggcgaacaa agagaaaaca ggaacttgga aaattcagca taaattaacc gccgaacaag
    23881 ttttaaataa aactattgtc ttattcaact atgtgtatga aaataaggaa gcttttgaag
    23941 caggcgataa gccagtagcg aaggatgtta gcttgaataa ccaagcccaa actgtcagtt
    24001 gtacagtgga acaccatgtt tctatccaaa caaaggccca cctagaaaac ggttctcaga
    24061 cctttactca tggtgatgtg gtggatatgt ttgatgatgt gtcgattact catgatgtac
    24121 tggatggctc aaaagaagct ttcgaaacaa ttctgtatgc tttattaccg gatggtacaa
    24181 acaaagaaat ttggaaatct ggcaagattg attatgaagt aaatgataaa gaattcacca
    24241 aaactgtgct tgcgaaaaaa gtagataccg gaaaatatcc agaaggaacc aagtttactt
    24301 ttgcggaaat caattacgat aaagatggga atatcaatgg aaaacacaat gaagatttga
    24361 aagaaaaatc tcaaacctta acaccaaaag aagttccaac catactaagt acgccgaaac
    24421 aaccggaaac accaactgtt ccaagtggct ctcaagaatc tagtcccaca gtgaagacct
    24481 tccctcaaac cggggagaaa aattccaatg ttttactgtt cattggtttt actttgattt
    24541 ttgcgacggc gggctattat ttctggaatc gccggaacta aggtgatgag atgatacaag
    24601 taaatcctaa gaaccaacgt gcgcgaccgc ctcctaaaaa agtagtcgcg ctatttttgt
    24661 atccaaaact aacaatgaaa agaggaaaag aagatggaat tgaaatttgt cgtgccagat
    24721 atggctgaaa cctttggaaa gttaagttat gccggtgaag gagaaattat aacagaagga
    24781 tatggacgga atactacggt gattggtcgc agttatcatt tgtattccag taagcaacgg
    24841 gccgatgata ttgaggtggt agtagctgcg gaagccggtg aaaaggattt tgatcaagac
    24901 caaccgctaa aagccgtgaa tccccatttg gttgccaaag gctatgagat tgagaatcgt
    24961 gggtttactg attacgtgtt gtatgttgat gatttagtga aagcatagga ggaaaagaag
    25021 atgagattaa cagaaggtat tgtcgtagat tcaggtttaa catttgggaa gttgcgtttt
    25081 tccgcattac gccgagaagt acggaagcaa aatgaggatg gaacaattgg taatgaggta
    25141 aaagaacgaa cctataattt aaaatcctct gcacaagggc gaatgattca agttagtatc
    25201 ccagctaatg tacccttacg cgagtttgct tatgatgcag aggtagagtt ggtcaatcca
    25261 atcgtggata ccgttgccaa ttatgttttt cgagaaggaa ctaccgttaa ttggttcatt
    25321 aaggcggatg atttagtctt gaagcggcag ccgaatcaag gaaatcctac gaaccaaaat
    25381 gaaagaaaga aataggtgaa gattatggaa attaatgttg aacatatttt agattgtctc
    25441 gatcaatatg gaaaaggcga gctgactgag gaacaactcg ttaaagcatt aacctctgat
    25501 gaaaaaatgt ttctgattat acatcaaggt ttacttggag aaaatagcga cagtgaagaa
    25561 gactctgatg tactagactg gttaggagaa gaagagtctt tctttatagt tgtcgaagta
    25621 aatgaaaact tatgtcggca ggcagaatcc attttggaag aaattggtgt ggagatgccg
    25681 gatgctattg aaggcttttt aaatcaattg gttgaaacga aacaactccc ctcctcgtag
    25741 cagtaaatga ctagcataac tttttgaaat tgcctaatga aatcctattg ggctttttcg
    25801 attggaggtg gaaaccatca aaatgtacaa aggacatcgc atacgcgcag gagatcagca
    25861 cttggtttat cactttgttc ttggctggct actcgctctg ttcatcggtt ggatgagcgt
    25921 cttttatttt caagagttta gacaatttga tatttctaag ctatcacttt ctactattga
    25981 aatcgtttgg tctataaaag atttagtatg tctgcttgga agtctcgctt tttcaggagc
    26041 tatgatattg ctttatattc acttttttct tgatcattgg cgaagcttgt ggcatcgaca
    26101 aaagctagct cggatgattc tagagaacca ttggtatgaa gtgaaacaaa ctcaaagtga
    26161 aggctttttc aaagatttga atagtagccg aaccagagag actatcagtt acttccccaa
    26221 aatctgttat cgaatgaaag atggattact gtctattcgc gttcaaattt cacttgggaa
    26281 ataccaagat cagcttttaa aattggaaaa gaagttagaa agcggactgt attgtgagtt
    26341 agtagaaaag gaactcaaag actcctatgt ggagtatact ttactgtatg atatgatcgc
    26401 caatcgaatt gggatagacg aagtagtggc agaaaatggg actctgcgat tgatgaaaaa
    26461 tcaagtatgg gcttatgatt ctttacccca tatgttaatc gctggtggca cgggtggagg
    26521 gaagacctat ttcctcctca ccattatcga agcactattg aagtcagatg cagaattgtt
    26581 tatcctcgat cccaaaaatg cagatttagc tgatttaggt acggtgatgc ctcatgttta
    26641 ttcacaaaag gaagaaattt ctgcttgtgt ggaagatttc tatgaacgta tgatggctcg
    26701 tagcaaggca atgaaagaaa tgtctaacta caaaacagga gagaattatg cctatcttgg
    26761 acttccaccc aactttttaa tctttgatga atacgtggct tatatggaga tgttaacgac
    26821 gaaagaaagt gcggtgattt tgaataagct caaacaaatc gtgatgttgg gtcgtcaatc
    26881 tggtttcttt ctgattttgg cttgccaaag gccggatgcg aaatatttag gagacggaat
    26941 ccgcgatcaa ttcaactttc gcgtggcttt gggtcgtatg agtgaactcg gatattccat
    27001 gatgtttggg gaggtcgaaa aaaacttctt tatgaagcac atcaaaggtc gaggttatgt
    27061 ggatacaggt ggcagtgtga ttagtgagtt ttatacgcca cttgtaccaa aagggtatga
    27121 tttcttagat tcaattagta aattagaaaa gatgaatgat tgagtgatct tcaatactga
    27181 ctttatactt caagtaacgt ataatacttt tatatagtag gaagaaaggg gataattaat
    27241 atggaaagta atgattctta tttagagtat gcaaaagcaa ataaggataa gtttatttct
    27301 tatattatca aagggaaatc ccccgaagtt ggacgagacg cagtttttat ggcagggagt
    27361 cctggggctg gaaaatcaga ggttgccatt ggattagctg agaattatga taaccatgtg
    27421 attattgatg cggattactt tcgcacccag tttcctgatt ataatggaaa aaactctagt
    27481 ctctttcaaa aagcatcttc ttggttggta gagcaggcat ttagatatgt cttagatcat
    27541 ggctattctt ttattctgga tgcaactttt gcaatattaa gtgctgaaaa aaatatctcg
    27601 cgggcgttga aaaaacattt tcgagtgacg attttctatg tttatcaaga ccctaaaatc
    27661 gcttggaaat ttacacaaga acgtgaaatt gcagagggac gtcatgtgcc gaaaaaggca
    27721 tttattaacg cttttttcaa atcaagagag aacattgcaa aagtaaagag taaacatcca
    27781 gatgta
//
LOCUS       DAZKLD010000020        27463 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.020, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000020 DAZKLD010000000
VERSION     DAZKLD010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27463
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.020"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(28..972)
                     /locus_tag="ACG8KR_000743"
     CDS             complement(28..972)
                     /locus_tag="ACG8KR_000743"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004459 - L-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HFQ0200141.1"
                     /translation="MKKTSRKVVIVGTGFVGTSIAYAMINQGISNELVLIDVNQEKAE
                     GEALDLLDGMAWGDENVAVWSGGYEECKDADIVVITAGINQKPGQSRLDLVKTNASIM
                     RQIVKEIMGSGFDGIIVVASNPVDILTYIAWNESGLPTSRVIGTGTTLDTTRFRKEIA
                     LKLKVDPRSVHGYILGEHGDSEVAAWSHTTVGGKPVFEIVEKDHRIAQDELDVIADKV
                     RNAAYEIIDRKKATYYGIGMSTARIVKAILNNEQAVLPVSAYLTGEYDEKDIFTGVPS
                     IVDENGVREVVELSINEEEKAMFSKSTSALREVLNTVL"
     gene            complement(1268..2212)
                     /locus_tag="ACG8KR_000744"
     CDS             complement(1268..2212)
                     /locus_tag="ACG8KR_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFQ0200142.1"
                     /translation="MRNEESIETLQREQGMNINDLNLAYHLQTPPKIHRGDLFQQGNI
                     HISKHRRYAEVPEHTHDFVEINYMYSGTCLQIINGERIEMEESDVVIIDKEATQRIEY
                     TGQEDILINILLKEETLSTEILNHLASTNNLLSSFLINASKEKGEHTQFIHFRGGKTS
                     RLTHLIENMLLIYFDTYSHKIRSLNLYTSLLLIELTHLLEQQSFSSADGCAENEMLRV
                     LDYIDKHFTSCTLKETAAHFGYNPVYLSNKLKMYFDASFQELVLKKKFDFALELIQET
                     SFSLEEIANRIGYKQTTSLFKLFKKYSTCTPNEWRNKH"
     gene            2433..3647
                     /locus_tag="ACG8KR_000745"
     CDS             2433..3647
                     /locus_tag="ACG8KR_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFQ0200143.1"
                     /translation="MLMNKKMMKFSLLSVSLILTSAGAVTSAIPSMSATYATRSLSAV
                     ELLTTAPSFMVTIFVLISSFIARKIGQKQTVIIGLVIASICGILPLFSQSYSIVLASR
                     AGLGIGFGLINSLAVSMIAAFYHGDERASMIGFQSAFQGFGAALMTFVAGQLVQFGWQ
                     YTFLVYAISLPILVLFVLFVPTPETTELQSHGTAPTRQVIKTNPKMISYGIFLLLVIV
                     VYNAVSIKLATVLTTYSLGNETNAANILTIMQLASMAAGVCFGWLQTKAHRYMLTLSL
                     LLMGIGFVLIATVINLYVIGFGAILTGISFSLFIPYLFNQVSIQAPKYAESFNTSILL
                     VGANLGTFIAPYGLLFLSFASLGSKTLAVFVNGSILLLISALISLLVVHRTNRKTVIR
                     VDEEIKEQLTTK"
     gene            3669..5684
                     /locus_tag="ACG8KR_000746"
     CDS             3669..5684
                     /locus_tag="ACG8KR_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HFQ0200144.1"
                     /translation="MTNEKHKDRWLWYPGDFEIRHGLLQNFQREERGFDWPAYWYMDD
                     CHRNVKFKRYYFLDQPSMFKVTIQGVGYVEINGQKHPCGKWLTCPAGKAKIRIFVGHT
                     SGLPAMFIEGDEVKSDIGWTASNFIEEYPAGWSPLYVDIAKDPNQIYYQKECVHPVTE
                     TEIGGGILFDFGRAVNGCVELISLDDHIESITLCYGESDVEAMDIDYCYYKQIGMTRK
                     MKARKRAFRYLFIPEVSTGMVQLQVFHEFLSLSKQAAFSSDSSLLNQIWSVSEEAFKL
                     CSDLFFIDGIKRDRWIWSGDAYQSFMINQYLFFDEEINKRTILALRGQNEIKQHMNTI
                     VDYSILWLIGIENHYMMTKDYEFLRIVYPKMQSLMRYLMEQTNELGFIYGRAKDWIFI
                     DWSEMDKEGTLAAEQVLLLKAYHSMITCGDILAETYGENENYHTHLYQEKYQLLKQNL
                     FDYFWDEELGAFIDCYESGRRNVTRHANIFAVLFDFANKDQQQSILKNVLLNDTITQI
                     TTPYFKFYEQDALCKLGQTNIVYQTILDYWGGMLSHDAVTFWEEYDPTQTGNEKYSMY
                     GDPYGKSLCHAWGASPIYLLGRYFIGLYPTKPGYEEFMIQPNLDSFETLNCTLPIKDG
                     KISLEKTKDKIVITSDRTGGTVKMNGKSYPLLAKQPLVLTSYSKINA"
     gene            complement(5767..7140)
                     /locus_tag="ACG8KR_000747"
     CDS             complement(5767..7140)
                     /locus_tag="ACG8KR_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFQ0200145.1"
                     /translation="MDKLLNLFVSIVSQPSILVSLIALLGLVLQKKKFSEVIQGTIKT
                     FVGFLVLIGGAGLISNSLTPFASMFQTALHTQGVVPSNEAVVAIALQQYGTPTALIML
                     VGMIVNILLARFTRYKFIFLTGQAMLYVSCLTAVILVSAGFTANLWMILLGGVFEGTL
                     LTITPALCQPFMKNITGNNQVALGHTGNIGYASSGWIGKFFGNKDRSTEDIKIPKSFS
                     FLRDSTVSIMLLMSIVYVALALLAGPGFVERELSNGDNAVIFALIQAGTFTAGFVIVL
                     QGVRMVLAEIVPAFQGIAKKLVPDSKPALDVPIVFPYAPNAVLIGFFVSFFVGTLSMF
                     AMIGLQTTVIIPGVVGHFFCGAASGVFGNSTGGRRGAVLGSAFNSLLISWLPLFILPV
                     LGDLKIASSTFADTDYLVPGLVLGKLGEYGQIALTVGIIAFFLLVVLSSFLFAGKRTN
                     IVSKEQN"
     gene            complement(7166..7450)
                     /locus_tag="ACG8KR_000748"
     CDS             complement(7166..7450)
                     /locus_tag="ACG8KR_000748"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFQ0200146.1"
                     /translation="MKIFAVCQSGLGTSFMVEMNIKQALADLGADVDEFEVSHTDVGS
                     VSSDMADYFFIEHSLKDTLTSIPDEKLVPLQSIIDSEEVKEKVTKILSKE"
     gene            complement(7452..9467)
                     /locus_tag="ACG8KR_000749"
     CDS             complement(7452..9467)
                     /locus_tag="ACG8KR_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFQ0200147.1"
                     /translation="MNYKISYLLKTPREISLEKAAEIIEVNTSEAIKQLTPFAEYVDD
                     TRIRIKKFRAIDWAEKFLDITRFEAVYTEQERRDLLYLMVYSTATSVSVFHFQDFLQV
                     SKGTVLSDIKKIRNELEVEDCQLSYTRKTGFRLLGSEAILRREAKNRTARLMQSLAGR
                     FGLTYLTATLSIDYYAKVRDLLDKTISLSDLRFAPNRMDELAYYLSLTTIRIGKTSME
                     LVKDAELLASLAVTDISRQVLTGLGYSEHQNSEIIFFSLCLASIVEGDVQEPALDFLL
                     ECSSEVIHRMEFLMAVEFASFRELLMNVFYHLVPAYFRISYSFYLPNVMIDQIKHQYA
                     SIYEMTRKALLPLERRIGKSIPEEEIGFFTILFGGEIRKADAEERNRKIRAVIVCPSG
                     ISSSLILKSELQQLFPMIFFTETNSADRLHEVDETSYDIIFSTVPLTLTDKKKSLYVL
                     HPIMTDLEKNQLINQVQRDWLIPGFSMPSAQELLSALLPYIELKEGVDEEKLYQVLNR
                     KMNKLLEKKEDQRPMLSELLTEEMIQLSDQPKNWQEAITFAAQPLLSENKIEESYIKA
                     MIERVEQYGAFIHIGDHIALPHARPEDGVNEVGMSLLKLQQPVDLLDNPAHPISLFIC
                     LAAIDNEAHLRALANLTKLLSNKQNLQDLLHASTKEEILAIIRKGDE"
     gene            complement(9646..9951)
                     /locus_tag="ACG8KR_000750"
     CDS             complement(9646..9951)
                     /locus_tag="ACG8KR_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA processing protein"
                     /protein_id="HFQ0200148.1"
                     /translation="MTNYDGEKHKRLRGSMAPKTEEEVALAGTNSGETEDKEESLAGT
                     NSGETENKETALSGSNSVEEETKPNEPNHFEKDIEKAVEDVKEKFEELTSKNKHDHH"
     gene            complement(10040..11071)
                     /locus_tag="ACG8KR_000751"
     CDS             complement(10040..11071)
                     /locus_tag="ACG8KR_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFQ0200149.1"
                     /translation="MLTIAYLGNGKSTNRYHLPFSRKLTNKINIKKIFSPSEPVWKTF
                     EDIEYTDQLSDLWDDPEIQLVVITTPSQFHYEYGKMALEHGKNVLVEKPFAETSKEAE
                     ELFALAKEKGLFIQCYQNRRFDSDFLTVQKVIESGKLGDILEVEMHYDYFRPEVPENT
                     HEFSTANSYLYGHACHTVDQVLSYFGEPDDISYDVRQLLGKGRMNDYFDLDFFYGNMK
                     ISIKSSYFRIKERPSFVVYGKKGMFVKQTKDRQEEHLKMFYMPDHEDFGIDLPEHYGV
                     LTYIDEQGSYHEEKVISEVGDYSRVYEGIYETLVNGAEKVIKDEETILQMKILEEGIK
                     SIQESEGKI"
     gene            11278..12153
                     /locus_tag="ACG8KR_000752"
     CDS             11278..12153
                     /locus_tag="ACG8KR_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFQ0200150.1"
                     /translation="MLIEEKLTKQELFTTTEKRIVDYIRRNIEAAVYMTIEELAKATY
                     TSHSAIIRLCKKMGFSGFKEFRFELGREVHQLIAQFNQTIDPNFPFSSEDSTQQIAKK
                     MADLSIQSIKKAQLQIEHEDLDQIGKVLNKAQRIFLFAKGDSQITARKFQNKMVKLNK
                     FLIMAEEYSDSSWNAANLTSEDCAIFISYSGRIHHYERIMTYLKHVEAPTLLITGNQH
                     SEMAKQADMCLVISQEEYDFAKVATFSSQIAFDYVLNTLFSVIYSQNFEENIRNLKNK
                     QELMQKGLLKEYRKT"
     gene            complement(12305..13147)
                     /locus_tag="ACG8KR_000753"
     CDS             complement(12305..13147)
                     /locus_tag="ACG8KR_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFQ0200151.1"
                     /translation="MKPKKRKLLDLLMLFLLLSGIGILAYPFVSDALNNYLDQQIISH
                     YQQQAVKENEEVMAKIQENMTKKNQQLAKEGGNPGADPFTKKKEPAKKDRTYFEKHTI
                     GILTIPEINVHLPIFDETNTVLLEKGASLLEGTSYPTGGENTHAVISSHRGLPQAKLF
                     TDLPKLTLGDSFYIEINGRTLAYQVDQIKTVEPTDTKDLRIEKGQDFVTLLTCTPYMV
                     NSHRLLVRGHRVPYHAKEVQKEVQKVSENKRFFLYGLLAACILIFLLLIYLFRQSFKI
                     RNRS"
     gene            complement(13239..15116)
                     /locus_tag="ACG8KR_000754"
     CDS             complement(13239..15116)
                     /locus_tag="ACG8KR_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFQ0200152.1"
                     /translation="MKNHKKINVMLGVLFLILPLLTNSFGAKKVFAEETAAQVILHKK
                     KMTDLPDPLIQNSGKEMSEFDQYQGLADISFSVYNVTQEFYAQRDKGASVDAAKQAVQ
                     SLTPGTPVASGTTDADGNVTLSLPKKQNGKDAVYTIKEEPKDGVSAAANMVLAFPVYE
                     MIKQADGSYKYGTEELDTIHLYPKNTVGNDGTLKVTKIGTAENEALNGAEFIISKEEG
                     TPSVKKYIQSVTDGLYTWTTDQTKAKHFITGHSYDIGNNDFAEASIEKGQLIVNHLEV
                     GKYNLEEVKAPDNAEMIEKQTITPFEILANSQTPVEKTIKNDTSKVDKTTPQLNGKDV
                     AIGEKIQYEISVNIPLGIADKEGTQNKYTTFKLIDTHDAALTFDNDSSGTYAYALYDG
                     NKEIDPVNYSVTEQTDGFTVSVDPNYIPSLTPGGTLKFVYYMHLNEKADPTKGFSNQA
                     NVDNGHTNDQTPPSVDVVTGGKRFVKVDGDVTSDQTLAGAEFVVRDQDSDTAKYLSID
                     PSTKAVSWVSAKESATVFTTTSNGLIDVTGLKYGTYYLEETKAPEKYVPLTNRVAFTI
                     DEQSYVTAGQLISPEKIPNKHKGTLPSTGGKGIYVYIGAGVVLLLIAGLYFARRKHSQ
                     I"
     gene            complement(15113..16534)
                     /locus_tag="ACG8KR_000755"
     CDS             complement(15113..16534)
                     /locus_tag="ACG8KR_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFQ0200153.1"
                     /translation="MKKLGWLSMCLFLLLFKPAFTQVATETETEMVQITLHKLLFPNG
                     QLPKNHPNDGQEKALLQTYRGLNGVTFQVYDVTDSFYHLREKGKTVEEAQAEIAKNGA
                     SSGMFTAEATTTTLNNEDGIASFSLAAKDQEKRDKAYLFIESKVPEVVKEKAENMVVV
                     LPVHGQNNQKLSTIHLYPKNEENDYPDPPFEKVLEEPRNDFTIGEKITYSLHTTIPVN
                     ILDYQKFELSDSADEALTFLPNSLTISSNGEKLTEGFVIHKKPHGFDVLFSIPSLEKY
                     AGKKLTISYQMQLSSTAQANKEINNNGTLDFGFGVSTKKVSVYTGSKQFVKIETNKPD
                     KRLAGAVFLIKNKAGNYLQQTANGYKWTKNESDALHLISDKNGAFSISGLKTGSYRLK
                     EIEAPSGYILSETEIPFTISTFLSEDKEADSILKVVNKKENSRPFLPKTNETKNTLLG
                     VVGMVFASFAIWLFIKKRTGVKK"
     gene            complement(16537..19926)
                     /locus_tag="ACG8KR_000756"
                     /pseudo
     CDS             complement(16537..19926)
                     /locus_tag="ACG8KR_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331773.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
     gene            20183..21574
                     /locus_tag="ACG8KR_000757"
     CDS             20183..21574
                     /locus_tag="ACG8KR_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0200154.1"
                     /translation="MEKIIRILSISDKWMTKKELAKRIGSSESTFVRYIEEIKIRWGT
                     DVTIQSSQKLGYRLEHLNVPAYLNVLMEMSQSSTNSRLLNEIIVNPGKTIEYYCEKIS
                     ISRSSFTRKLKQCNQILESYLLKVIVDQGFQLTSLKSELQLRIFVTFFFLSYYGRRTL
                     PFPIEKKKIKEIMVKNNCQMNLFQEESPYEQSFFIMYFVVHLMRESQGTPFEWKAGDS
                     YVQLINIDKKDFLILKNLLGKLKYSSYIKVVNYFVNTIFSEFSEKEKNEISEQIRKNM
                     SYCSFSSADCKEKNEEYVVQLLTQIYFFSRIFPYDTTHLTIRATYFAQQMQHRQIRLY
                     AGFRAELQFYSTLLNQNLIYYLSSYIFWMLIGNPEIHHYTSDKKILIISDLSLRHSQY
                     IEEYISDILAVHKIHSETTAITEDQLSKYNLLEYDLIVTNQPILNAHVPHILIDDSVS
                     FANEEELIRLFEL"
     gene            complement(21647..22294)
                     /locus_tag="ACG8KR_000758"
     CDS             complement(21647..22294)
                     /locus_tag="ACG8KR_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFQ0200155.1"
                     /translation="MTMKGYPRIIRDKGEMWMPKETFFNLSNAKKQRIDDLLLEIFCS
                     QHISQVKVSEIVDKMGMSRGAFYKYFQDLEDAYLYTIKSSSHIVHQDILKFISENKQH
                     FFLGIEKYLEWCCDLDHESIYWQRLYLLTQRNDQQTTKRRELSADSPMNEKWLELLRI
                     NHFSIQSSEEAVSFLYFSMTLVMNTLTDLIANNWTKEELLKDFRYKSRWIAEGIQ"
     gene            complement(22302..22988)
                     /locus_tag="ACG8KR_000759"
     CDS             complement(22302..22988)
                     /locus_tag="ACG8KR_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0200156.1"
                     /translation="MKKILTMKKVTKTFGSGHAKVSALKAIDFYVKEGEFVSIIGPSG
                     SGKSTFLTISGGLQTPTSGEIVINGHSLTELPEKKRADLRFKEIGFILQSSNLIPFLT
                     VKEQFELVDQVTSKKEDSTKIDSLLQSLDIQSLKNSYPKDLSGGERQRVAIARALYNE
                     PSLILADEPTASLDTDHAYEVVKLLVKEAHEKQKATIMVTHDVRMIQWSDRVYRMEDG
                     YLTEEEKKKE"
     gene            complement(22994..24067)
                     /locus_tag="ACG8KR_000760"
     CDS             complement(22994..24067)
                     /locus_tag="ACG8KR_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0200157.1"
                     /translation="MFLAIKEIRYSKLRYGLIVGIMFLIAYVVFMLSGLANGLSEEFK
                     KAIDDWDAQEIVLSEDANQVFAASQLTRGDLKYIEGGEKAPIGLYSGAIKGKNKENVT
                     VFGTTKEAFLLPKLTKGHEFKTKNEIIISQNLADSGYKIGDTIKIGSYDDPLTIVGIF
                     PETYYTVSPVIYASLDTWTALKYGQQPFASEEEQPINGIVIKDSAKISKEKASKGLQL
                     LTIGTFIESIPGYSAQNMTLNAMIYFLFLVVAAVVGIFMYVMTLQKTAIFGVMKAQGI
                     KNFFIAKSLVAQSFIVGVVGVLLALLFAYLTSLILPSAMPFAVFWSQWLLYSGILIIV
                     AMLGGLFSIRTITKVDPITAIGG"
     gene            24289..24963
                     /locus_tag="ACG8KR_000761"
     CDS             24289..24963
                     /locus_tag="ACG8KR_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFQ0200158.1"
                     /translation="MIKLLVVEDDKVLSNNIKEILAAIGEITQVYDGSEGLFELESGV
                     YDLAILDLMMPEMNGYEVLAKIRKEKIQTPVLILTAKDSLEDKIEGFERGADDYLTKP
                     FYREELIMRVKALLKRSLGLFDENVLKYKDISCDLKSNEVLYKEQVLPIQGKEFELLV
                     YFLQNKGIILTKEQIFDRIWGFDSDTTITVIEVYMSNLRKHLRSTGLDKEIKTLRNVG
                     YILQRV"
     gene            24968..26308
                     /locus_tag="ACG8KR_000762"
     CDS             24968..26308
                     /locus_tag="ACG8KR_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HFQ0200159.1"
                     /translation="MNGDITRKQRSRFFATNVAAFAFIFFLLGFITLQILNQSAYRET
                     DLSLEKINFDSRTVQLEIQRYQQGDPFLNPTQTEQNRTNMEQPLMEPSNDRFNTQVIL
                     WSKNGEILNKAAIGGRFNQIKELKMDTKNLNTIQNIELNEDSGSSALSFRSITKKAET
                     NQDGVAYIQILENTNQIKNSLATFRTIMILCMIVFWILSIGISYYLSSLSMRPIILSW
                     RRQKEFVENASHELRTPLTIIQNSLEHLFTKPNHTIIEESESIAQALSETRRLTGLTS
                     DLLTIARNDSDQQLLSKQMINTQEYIEQLVKPFQEMAIIDGKEFILHNDATLEVPIDE
                     KKIHQVLVILLDNALKYTNPEDKIILDSSVNHKEWLIHVKNTGPSISEEDQARIFDRF
                     YREDQSRAKETGGYGLGLAIAKQVIEQHHGQISVSNYLPQGVDFKVQLPLKPKK"
     gene            complement(26457..27443)
                     /locus_tag="ACG8KR_000763"
     CDS             complement(26457..27443)
                     /locus_tag="ACG8KR_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HFQ0200160.1"
                     /translation="MEEKNLTVIGSYSTREEALSVIERLRNEGYERDDIVIYTTDEAA
                     SRLGFDGLSGIDVETDENRMDGEEDRSLWEKIKDTFSFDTYDSETATPENDPLYQYRG
                     DISDGKFVITVKGYRQPETTEDTMDTQDTVSPASTMGTGLETGTGTDPMDVGGTTGFQ
                     NTTDPLNMTPGTDPMDVPGETDTMGTSKTEDIDRKPNLDDDTIQLKEEKLDVNTHDVT
                     TGEVDIHKHVVNDTETVEVPVKREEIVIERKPVTDQSSQGTDEKLEDDTITIPIKEEQ
                     VDVSKHTVIREEVGIHKEEHEDVEKVTEDVSREELDIDTSGDVHIEDRNKKS"
BASE COUNT         7993 a         5208 c         4998 g         9264 t
ORIGIN      
        1 ggatttttag ttcatattca ttgtttctta taatacagtg ttcaatactt ctcttaaagc
       61 acttgttgat ttgctgaaca ttgctttttc ttcttcgttg attgataatt caacaacttc
      121 acgaacacca ttttcgtcaa cgattgatgg cacacctgta aagatatctt tttcgtcgta
      181 ttcaccagtt aaataagcag atacaggcaa tacagcttgt tcgttattca agatagcttt
      241 tacgatacgt gcagtactca taccaatacc atagtaagtt gcttttttac gatcaatgat
      301 ctcataagca gcattacgga ctttatcagc aatcacatct aattcatctt gggcaatacg
      361 atggtctttt tctacaattt caaacactgg tttaccaccg acagttgtat gtgaccatgc
      421 agcaacttct gaatcaccat gttcacctaa gatatagccg tggacgctgc gtgggtcaac
      481 ttttaatttc aatgcgattt ctttacggaa acgagttgta tccaatgttg ttccagtacc
      541 gataacacgt gatgttggca gaccagattc gttccaagcg atatatgtca aaatatctac
      601 tgggtttgaa gcaacaacga taataccgtc gaatcctgat cccatgattt cttttacgat
      661 ttggcgcatg attgacgcat ttgttttaac taaatctaaa cgagattgac caggtttttg
      721 gttgatacca gctgtgataa caacgatatc agcatcttta cattcttcat agccacctga
      781 ccatacagcc acattttcat cgccccaagc cataccatct aaaaggtcca atgcttcccc
      841 ttctgctttt tcttgattca catcgatcaa tactaattca ttagagattc cttggttgat
      901 cattgcataa gcgatacttg ttccaacaaa acctgtaccg acgattacta ctttacgact
      961 tgtttttttc atgaataaac agctcctttt cataactatt tgttactttc atgaaaagta
     1021 tagtcttgtt tattcatttt atcaacatgt taattataat agttgttatt ataatttttg
     1081 atggaaacga attcctgaat acgcataaca gataaatgac aaatgtttac acctatatcc
     1141 cctgataata caaccaaaag tttagaactg aaaataaatc caaaaaaatt cgctgcaacg
     1201 atataataag acagcaatcg cacctaaaac aattttcctt catatgcttt agtgaaagat
     1261 tttcttctta atgtttattt ctccattcat tgggagtaca tgttgaatac ttcttgaata
     1321 atttgaataa agaagtcgtt tgtttatatc cgatacgatt agcgatttct tccaacgaaa
     1381 aactcgtttc ttgaatcagt tccaacgcaa agtcgaattt tttctttaga accagttctt
     1441 gaaaggaagc atcaaaatac attttcaatt tattactaag atagaccgga ttgtagccaa
     1501 aatgagctgc tgtttctttt aacgtacaag aagtaaaatg cttatcgata taatctaata
     1561 ctctaagcat ctcattttct gcgcatccat ctgctgatga aaagctttgt tgttctaata
     1621 aatgagtcaa ttcgatcagt aatagtgatg tatagagatt taacgaacgg atcttatgac
     1681 tgtatgtatc aaaataaatc aacagcatat tttcaatcaa atgcgtcaaa cgacttgttt
     1741 ttccgccgcg aaaatgaatg aattgtgtat gttccccttt ttcttttgat gcatttatca
     1801 aaaaagaaga aagtagatta tttgtgcttg ccaaatgatt caatatctct gtagataagg
     1861 tttcttcttt taataagatg ttgattaaaa tatcctcttg ccctgtgtat tcgattcttt
     1921 gagtagcttc tttatcaatg atcaccacgt cactttcttc catctctatc ctttctccat
     1981 taataatttg taaacatgta ccgctataca tataattgat ttcaacaaaa tcatgtgtat
     2041 gctcgggaac ttccgcatat cgacggtgtt tactgatatg aatatttccc tgttgaaata
     2101 gatcccctcg gtggattttt ggcggtgtct gaagatggta agctaagttt aaatcattga
     2161 tattcattcc ttgttcacgc tgcagagttt cgattgattc ttcgttacgc aaaaaaaatg
     2221 tctaattcag ctagttccaa ttgactccct cctttttgta agcggtttct ttcgcttatt
     2281 agttcacgct gtactgctat tatatgcaaa gctatttttg gtttcataaa gactttctat
     2341 ggttgggaca tgaacttcct gtgattccgc tattgagaga ataaccactt catactatat
     2401 ttttgataag cgcttacaaa aatgaaagga acatgcttat gaataaaaag atgatgaaat
     2461 tttctctgct gtcagtctct cttattttga cttctgctgg tgcagtcacg tctgcaattc
     2521 ccagtatgtc agctacttat gccacgcgtt ctttatctgc tgttgaatta ttaacgactg
     2581 ctccctcttt tatggtcact atttttgttt tgattagtag ttttattgct aggaaaatcg
     2641 gacaaaaaca aactgtgatc atcggattag tgattgcatc gatttgtggt atcctccccc
     2701 tcttctcaca aagttacagt attgtcttag cttcaagagc tggactaggt attggctttg
     2761 gattgatcaa ttctttggca gtcagcatga ttgctgcctt ttatcacgga gatgaacgag
     2821 cctctatgat tggttttcaa agtgcatttc aaggatttgg tgcagcttta atgacatttg
     2881 ttgcaggaca gctagtacag ttcggttggc aatatacctt tttagtctac gccatcagct
     2941 tacctattct tgtcttgttt gttttgtttg ttcctacacc tgaaacaact gaacttcaat
     3001 cgcatggcac agccccaaca agacaagtaa tcaaaacgaa tcctaaaatg attagttatg
     3061 gtatttttct cttgctggtc atcgtggtat ataatgctgt atcgattaaa ttagccacag
     3121 tcctgacgac ttattctcta ggaaacgaaa ctaatgcagc gaatatctta acaatcatgc
     3181 aattggcaag tatggctgcc ggagtttgtt ttggctggct tcagacaaaa gcacatcgct
     3241 atatgcttac actttcttta ctactgatgg gaatcggctt tgtattgatt gccactgtga
     3301 taaacctgta tgtgattggg tttggtgcga tcctaacagg aatttctttt tcgttattca
     3361 ttccttactt attcaaccaa gtaagtatcc aagcgcccaa gtacgcggag agtttcaata
     3421 cttcaatctt attggttggc gctaatttag gtactttcat tgcaccatat ggtttgctct
     3481 ttctctcttt tgcttctctt gggagtaaaa cacttgctgt tttcgtcaat ggcagtatct
     3541 tgctgttgat tagcgcgctt attagccttc tagtagtaca caggacgaat agaaagacag
     3601 tgataagagt agatgaagaa ataaaagaac aattgacaac aaaataaatt tgatggaggt
     3661 cgtgtaaaat gacaaatgaa aaacataaag atagatggct ttggtatcct ggagactttg
     3721 agatccgcca tggactgcta caaaatttcc aacgggaaga acgaggtttt gactggccag
     3781 cgtactggta catggatgat tgccatcgga acgtcaagtt taagagatat tactttcttg
     3841 atcaaccttc catgtttaaa gtgacgatcc aaggagttgg gtatgtagaa attaatggac
     3901 aaaaacatcc atgtggaaaa tggctgactt gtccagctgg taaagcaaaa attcgtatct
     3961 ttgtaggaca tacatcaggg ctaccagcga tgtttatcga gggagacgaa gtaaaatcag
     4021 atattggctg gacagcaagt aattttattg aagaatatcc agctggctgg agtccgcttt
     4081 atgtcgatat cgcaaaagat ccaaatcaaa tctattatca aaaagaatgc gtccatccag
     4141 tcactgaaac ggagattggc ggaggaatct tattcgattt tgggcgagca gtgaatggct
     4201 gtgtagaact aatatctctg gatgaccata tcgaatctat cactctatgt tatggagagt
     4261 ccgatgtaga agctatggat atcgattatt gctactataa gcaaatagga atgacaagaa
     4321 aaatgaaagc tcgtaaacgc gcttttcgct atttgtttat accagaggtt tctactggaa
     4381 tggttcaact tcaagttttt catgaatttt tatctctttc gaaacaagct gctttttcaa
     4441 gcgattcttc tttactgaat cagatctggt cagtatcaga agaagccttc aagttatgta
     4501 gtgatttgtt ttttatcgac ggtatcaaac gagatcgctg gatttggtct ggagatgcgt
     4561 atcaatcatt catgatcaat caatatcttt tttttgacga ggaaatcaat aaacgaacga
     4621 tattagcatt aagaggacaa aatgaaatca agcagcatat gaatactatc gttgactatt
     4681 cgatcttgtg gttgattggt atcgaaaacc actatatgat gacgaaagac tatgaatttt
     4741 tacgcatcgt ctaccctaag atgcaatcat taatgagata cttgatggag caaacgaatg
     4801 aattagggtt tatttatggg agagcaaaag attggatatt tattgattgg tctgaaatgg
     4861 ataaagaagg aacgcttgct gcagaacaag tcttattact gaaagcctat cattccatga
     4921 tcacttgcgg agatatctta gctgaaacat atggagagaa cgaaaattat catactcatc
     4981 tctatcaaga aaaatatcaa ctattgaaac aaaacctatt tgactacttt tgggatgaag
     5041 aacttggcgc ttttatcgat tgctatgaat ctgggagaag aaatgtgacg cgtcatgcga
     5101 atattttcgc tgttttattc gattttgcta acaaagacca acagcaatcg atcttaaaga
     5161 atgtcttatt gaatgacact atcacacaga ttactactcc atatttcaaa ttctatgaac
     5221 aagatgcttt atgcaaactc ggacagacga atatcgtcta ccaaacgatt cttgattatt
     5281 ggggagggat gttatctcat gatgcggtaa ctttctggga agaatacgac cctactcaaa
     5341 caggaaatga aaagtacagt atgtatggag atccttatgg aaaaagctta tgccatgcat
     5401 ggggagcaag cccaatctat cttttaggac gctattttat cggtttgtat ccaacaaagc
     5461 caggttatga agaatttatg atacaaccta atctagattc atttgaaaca ctgaattgta
     5521 cgctacctat caaagatgga aagatttcgc ttgaaaaaac aaaagataag atcgtgatta
     5581 cttctgatag aacaggcgga acagtgaaaa tgaatggtaa aagttatcct cttctagcaa
     5641 aacagccact tgtcttaaca agctattcaa aaataaatgc ataaaacaaa aaactgcttg
     5701 cgacatatca gaagacttta aatcttcttc tgatattgtc caagcaattt ttattttgaa
     5761 gtttgcttaa ttctgttctt tagaaacaat gtttgttctt tttccggcaa aaagaaagct
     5821 agataatacg acaagtaaaa agaaagcgat gattccaact gtcaaggcta tttgaccgta
     5881 ttctcctagt ttcccaagta caagtcctgg tactagataa tcagtatcgg caaatgtaga
     5941 agaagcgatt ttaagatctc ctaatactgg aaggataaat aatggcagcc aactaatcaa
     6001 taagctattg aaagcagaac ctaataccgc ccctcttcta ccgccagtcg agttgccgaa
     6061 gacaccagaa gcagctccgc agaagaaatg tcctactacc ccaggaatga taactgtagt
     6121 ttgcaagcct atcatagcaa acatggatag tgttcctaca aagaagctaa caaaaaagcc
     6181 aattaataca gcattaggtg catatggaaa aacgatagga acatctaatg ctggtttaga
     6241 atcgggtacg agttttttag caatcccttg gaatgctgga acgatttctg ccaacaccat
     6301 tcgaacccct tgaaggacga taacaaaccc agctgtgaat gttccagctt ggatcagtgc
     6361 aaagattaca gcgttgtctc cattgcttag ttctctttca acaaatccag gaccagctaa
     6421 caaagcaagt gcgacatata cgatcgacat caagagcatg atcgatacag tagaatctcg
     6481 caaaaagctg aaacttttag gaatctttat gtcctctgta gaacggtctt tgtttccaaa
     6541 aaactttccg atccatccac tagaagcata accgatattt ccggtatgcc ctaacgcaac
     6601 ttgattgttt ccagtgatgt tcttcataaa cggttgacaa agagcaggcg taatagttaa
     6661 tagtgttcct tcgaagaccc cgcctaataa gatcatccac aaatttgcag taaaccccgc
     6721 gcttactaaa atgactgctg ttagacaaga aacataaagc attgcttgac ctgtaagaaa
     6781 aatgaattta tatcttgtga atctagcaag taaaatattc acgatcatcc ctactagcat
     6841 gatcaaagca gtaggtgtac cgtattgttg caaagcaatc gccactactg cttcattgct
     6901 aggaactact ccttgagtat gcagagctgt ttggaacatc gaagcaaaag gggttagtga
     6961 attactgatt aaaccagcac cgccgatcag tactaggaat ccgacaaatg ttttgatagt
     7021 tccttggatc acttctgaaa atttcttctt ctgaagaacg agaccaagca gtgcaatcaa
     7081 agaaacgagg atggagggct gactgacgat tgacacaaac aaattcaaca atttatccat
     7141 tgatagttcc tcccttgaat catttttatt cttttgacaa gatcttcgtt actttttctt
     7201 ttacttcttc gctgtcaatg atcgattgca aaggtacgag cttttcatca ggtatagaag
     7261 ttagcgtatc tttcaaagaa tgctcaatga agaaataatc agccatatcg ctagatacac
     7321 ttccaacatc cgtatgactt acttcaaact cgtccacatc tgctccaaga tctgcaagtg
     7381 cttgtttgat gttcatctct accataaaac ttgttcctaa tccactttga catactgcaa
     7441 aaattttcat atcattcgtc tccttttctt atgatcgcta atatttcttc ttttgttgat
     7501 gcgtgtaata aatcttgaag attttgttta ttagaaagta gctttgttaa atttgccaaa
     7561 gcccgaagat gtgcctcatt atcgatagca gctaaacaga tgaacaaact aatgggatgg
     7621 gctggattat caagcagatc cacaggttgt tgaagtttca gcaaagacat ccctacttcg
     7681 tttacgccat cttctggacg agcatgaggt aatgcgatgt gatcgccaat atggataaac
     7741 gcaccatact gctccacgcg ctcgatcata gctttgatat aactttcttc tattttattt
     7801 tctgataata gcggctgtgc ggcaaatgtg attgcttctt gccagttttt tggttgatcc
     7861 gataattgga tcatttcttc tgttaataat tcagatagca taggacgttg atcctccttc
     7921 ttttctaaaa gtttgttcat tttacgattt aatacttgat agagcttttc ttcgtctacg
     7981 ccttctttta attcgatata aggcagtagc gccgacaaca attcttgggc agaaggcatt
     8041 gaaaatcctg gtatcagcca atctctttgt acttgattga tcagctgatt tttttccaag
     8101 tcagtcatga ttggatgcag aacatagaga gactttttct tatcagtaag tgtgagaggt
     8161 acagtagaaa aaataatatc gtaagaagtt tcgtcaacct cgtgtaagcg gtcagctgaa
     8221 ttagtctctg taaaaaagat catcgggaat aattgctgaa gttcactttt taagatcaat
     8281 gaagagctaa tgccacttgg acagacgatc acggctctta tttttctatt tctttcttct
     8341 gcgtccgctt ttctgatctc tccgccaaat agtatagtga aaaaaccaat ctcttcttca
     8401 gggatgctct tccctattcg tctttctaac ggaagtaatg cttttcttgt catttcgtag
     8461 atggaagcat attgatgctt aatttgatcg atcatcacat taggtaaata aaaagaatag
     8521 gaaatacgaa aataggcagg gaccaagtga taaaagacat tcatcaataa ttcacgaaac
     8581 gaagcaaatt ctacggccat caggaattcc atgcgatgga tgacttctga agaacattcc
     8641 agcaaaaaat caagtgcagg ctcctggaca tctccttcaa cgatcgatgc taagcaaaga
     8701 gagaaaaata taatctccga attttgatgt tcggaatagc caaggccagt caatacttgt
     8761 cgactaatgt cagtgactgc aagagaagcc aacagttcag catcttttac cagttccatg
     8821 gatgtttttc caattcgaat ggttgtcaaa ctcaaataat aagctaattc atccattcga
     8881 tttggtgcga atcgaagatc tgatagtgaa atcgttttat ctagcaaatc tctaactttg
     8941 gcgtaataat caatggaaag ggttgcggtt aaatacgtga gcccaaatct tccagcaagt
     9001 gactgcatca aacgagcagt gcgatttttt gcttctcttc ttaatattgc ttctgaccct
     9061 agcagcctaa aaccagtttt cctagtatac gacagctgac aatcttccac ttctaattca
     9121 tttcttatct ttttgatatc tgacaggact gtccctttag aaacctgaag aaaatcttga
     9181 aaatgaaaaa cagaaacaga cgttgcagtc gaataaacca tcaaataaag aagatctctt
     9241 cgctcttgtt cggtatacac agcttcaaat cgagtaatat ccaaaaattt ttctgcccag
     9301 tcgattgctc ggaatttctt tatacggata cgggtatcat ccacatattc ggcaaaagga
     9361 gttaattgtt tgatcgcctc gcttgtattt acttcgatga tctctgctgc tttttctaga
     9421 ctgatttctc ttggtgtttt caggagataa ctaattttgt agttcacagc gctgttcacc
     9481 tcctacttat attctagtat ggtagcgctt ttaaatcgag gggtgtttgg tacaacttac
     9541 agttggattt tttttacgac tgtaccgaac gatttctatc gaatggatac agaaaaaaag
     9601 caggtgacca aatgtctcct gcttttatga attgatttcg tccgatcaat gatgatcatg
     9661 tttatttttt gaggtcagtt cttcaaattt ttctttgaca tcttcaaccg ctttttcgat
     9721 atctttttca aagtgattgg gctcattagg ctttgtttcc tcttctacag aatttgaacc
     9781 agataaagct gtttctttat tttctgtttc accagagttt gtgccagcta atgattcttc
     9841 tttgtcttct gtttcaccag aattagttcc tgctaaagcc acttcttctt ctgttttagg
     9901 ggccattgaa cctcttaatc gtttgtgttt ttcaccgtca taatttgtca tgttggttcc
     9961 tcctagtagt tctatacttt ttcatcgtac aataagacag tttatgcgtc aaaaaacaaa
    10021 cccgattctt acaaatatgt catatttttc cttctgattc ttgaatgctt ttgattcctt
    10081 cttccaaaat tttcatttgt aagattgttt cttcatcttt gatgactttt tctgcgccat
    10141 taactaatgt ctcgtaaatc ccttcgtaga cacggctgta atcaccaacc tcggaaatta
    10201 ctttttcttc atgataactc ccttgttcat ctatataggt caatacacca taatgttcgg
    10261 gcaaatcgat accaaagtct tcatgatcag gcatgtaaaa cattttcaga tgttcttctt
    10321 gtcgatcttt tgtttgtttc acgaacatcc ctttttttcc gtaaacgacg aaacttggac
    10381 gttctttgat gcggaaataa ctggatttta tcgaaatttt catatttcca tagaaaaaat
    10441 ccagatcgaa gtaatcattc attcgtcctt tccctaaaag ttgacgtaca tcatagctga
    10501 tatcatctgg ttcgccgaaa taagaaagaa cttgatctac tgtatgacaa gcatgtccat
    10561 ataaatagct gttcgcagta gaaaattcat gcgtattttc tggtacttcc ggtctgaaat
    10621 aatcataatg catttctact tctaaaatat cccctaattt tccgctttcg attacttttt
    10681 ggactgtcaa gaagtcagaa tcgaaccgtc tgttttggta acattggata aatagacctt
    10741 tttcttttgc taatgcaaat aactcttctg cttcctttga cgtttctgca aaaggtttct
    10801 ctactagtac attcttacca tgttcgagtg ccatttttcc atattcataa tgaaattgac
    10861 taggtgtagt gatgacaact aattggatct caggatcatc ccaaagatca gacagctgat
    10921 cagtatattc gatgtcttcg aatgttttcc acactggttc agatggggaa aaaatttttt
    10981 tgatattgat tttgttcgta agttttcttg aaaaaggcaa atgatagcga tttgtactct
    11041 ttccattgcc aagataagcg attgtaagca tgagtggtgc ctccttttat tgatgttttc
    11101 agtatatcct agaaaagcgc agaaagtatt tataatagct attctggtaa aaaagttcag
    11161 aaaaacactt gttatcattc ggtttggtga aattaatgtt caaagtaagg ataaaaatca
    11221 aaaagatagt agcggaacca agtgtttatc agtaagatag aatttggagg tcagatcatg
    11281 cttattgaag aaaaactaac aaaacaagag ttgtttacca cgacagagaa acggattgtc
    11341 gattacatcc gtaggaatat cgaagctgca gtctacatga cgattgaaga attagcaaaa
    11401 gctacgtata cttcccattc agctatcatt cgtttatgca aaaaaatggg attcagcgga
    11461 tttaaagaat tccgtttcga gctggggcgg gaagttcatc aattgattgc tcagttcaat
    11521 caaacgattg atcctaattt tcctttttca agtgaagata gcactcagca aattgcaaag
    11581 aaaatggcgg atttatcgat ccagtcaata aaaaaagcac aattgcagat tgaacatgaa
    11641 gatttagatc aaatcggtaa agtattgaac aaggcacagc gaatctttct ttttgcaaaa
    11701 ggtgattctc aaatcacggc aaggaaattc cagaataaaa tggtcaagtt gaataagttt
    11761 ttgatcatgg ctgaagaata cagtgattct tcttggaatg cagccaattt gactagcgag
    11821 gattgcgcca tttttattag ctacagcggt cgcatccatc attatgaacg aataatgacc
    11881 tatttgaagc atgtagaggc ccctaccttg ctgattaccg gaaatcagca ttcagaaatg
    11941 gccaaacagg ctgatatgtg tcttgttata tcgcaggagg aatatgattt tgctaaagta
    12001 gcgactttct cctctcaaat tgccttcgat tacgtattaa atacgttatt ctcagtgata
    12061 tattctcaaa attttgaaga gaatatccgc aacttgaaaa acaaacaaga attgatgcaa
    12121 aaaggccttc ttaaagaata tcggaaaact taagaaaaga tcctcattta gtaataagaa
    12181 agatagtgtt tgataggctc tctttgatat catcataaaa acaggctgaa gttttctttt
    12241 tcaaacctca gcctgtttgt ttacggaacg attcgaatag atatattata gaaatgagga
    12301 atagctaact tctgtttctt attttgaacg attgacgaaa aagatatata agaaggagga
    12361 aaatcagtat acatgcagct aacaatccat acaggaaaaa tcgtttgttc tcggatactt
    12421 tctgtacttc tttttgaact tcttttgcat gataaggaac ccggtgccct ctgacaagta
    12481 agcgatgact gttgaccata taaggtgtac aagttaggag tgtcacgaaa tcctgccctt
    12541 tttcgattct cagatctttt gtatcagtcg gttctaccgt cttgatctga tcgacttgat
    12601 aagcgagtgt ccgtccattg atttcgatat agaaagaatc acctaaagtc agtttcggta
    12661 agtctgtgaa caactttgct tgaggcagtc ctcgatggct ggaaataacg gcatgtgtat
    12721 tttctccccc cgtaggataa gacgttcctt ccaataatga ggcacctttt tctagcagca
    12781 cagtattcgt ttcatcaaaa attggcaaat ggacattgat ctcagggatc gtcaaaatac
    12841 cgatcgtatg tttctcaaaa taggttctat cctttttcgc aggttctttt ttcttagtaa
    12901 agggatctgc acctggattt ccaccttctt ttgctaattg ctggtttttc tttgtcatgt
    12961 tttcttggat tttagccatg acttcttcat tttctttgac cgcttgttgc tgataatgag
    13021 agatgatctg ctgatccaga taattattca gcgcgtcgct aacaaatgga taagctagta
    13081 tccctattcc tgagagaagc aagaaaagca tcagcagatc tagaagtttc cttttctttg
    13141 gtttcatttg tgttctcctt gtgatgactg tttgataata aaagaaaaag gagcatttgc
    13201 gtaatccgct gctccttttt ccaattcttg caaaaagact aaatctgact gtgcttgcgt
    13261 ctagcaaagt acagtccagc aatcagtaga aggactactc ctgcaccgat atacacatag
    13321 attcccttac cgcctgttga aggaagtgta cctttgtgtt tatttggtat tttttcagga
    13381 gaaatcaact gtcctgctgt tacataagat tgttcatcga tagtaaatgc tacacggttt
    13441 gttaatggaa catatttttc tggcgctttc gtttcttcca gatagtacgt gccatatttt
    13501 agacctgtca catcgattaa accgttactt gtggttgtaa aaaccgttgc tgattctttc
    13561 gccgataccc agctgacggc ttttgtggat gggtcgatcg ataaatattt cgctgtgtca
    13621 ctatcttgat cacgaacgac gaattctgct ccagcaagtg tttggtctga tgtaacgtca
    13681 ccatctactt taacaaatcg tttgccccca gtaacgacat cgactgacgg tggtgtttga
    13741 tcatttgtat gcccgttatc gacatttgct tggttagaaa atcctttggt tggatctgct
    13801 ttttcgttca aatgcatata gtaaacgaat ttcaatgtac cgccaggagt taatgaagga
    13861 atataattcg gatcaactga aaccgtgaat ccgtctgttt gctcagtgac agaataattt
    13921 actgggtcga tttctttatt tccatcatat aaggcataag catacgttcc tgaagaatca
    13981 ttatcaaatg ttaaagcagc gtcatgagta tcgataagtt tgaatgttgt gtacttgttt
    14041 tgcgttcctt ctttatcagc gatacctaat gggatattga cagaaatctc atattgaatt
    14101 ttttcaccga ttgcgacatc ttttccattc aattgaggtg ttgttttatc aactttagac
    14161 gtatcatttt tgatggtctt ttctactggt gtttggctat ttgccaggat ctcaaaaggc
    14221 gtgattgttt gcttttcaat catttccgca ttatcaggag cttttacttc ttctaaatta
    14281 tattttccaa cttctaaatg attaacgatc aactggcctt tttcaataga tgcctcggca
    14341 aagtcattgt tgccgatgtc ataagaatga ccagtaatga aatgtttggc tttggtttga
    14401 tcagttgtcc aagtgtacaa tccatctgtg acactttgga tgtatttttt gacgcttggt
    14461 gttccttctt ctttagaaat aataaattct gctccattta gtgcttcgtt ttcggcagta
    14521 ccgatttttg taactttcaa cgttccatca ttaccgactg tatttttagg gtagagatgg
    14581 atagtatcta gttcttctgt cccgtattta taagagccat ctgcttgttt gatcatctca
    14641 tatacaggga aagctaaaac catgtttgcg gcagctgaca ctccgtcttt tggttcttct
    14701 ttgatcgtgt agactgcatc tttcccattt tgttttttag gtaaagataa agtgacattt
    14761 ccatcagcat ctgtcgttcc tgaagcaact ggtgtaccag gagtcaaaga ctggactgct
    14821 tgttttgctg catccacgga cgctccttta tctcgttgcg cataaaattc ttgagtgacg
    14881 ttataaactg aaaatgaaat atcggctaat ccttggtatt gatcgaattc gctcatttct
    14941 ttcccgctgt tttggattaa aggatcgggt aaatcagtca ttttcttttt atgaaggatg
    15001 acttgagctg ctgtctcctc tgcaaacact ttttttgcgc cgaagctgtt tgtgagtaat
    15061 ggtaaaataa ggaaaaggac tcctaacata acgtttattt ttttatgatt tttcattttt
    15121 tcactcctgt tctttttttg ataaacaacc agattgcaaa gcttgcgaat accataccaa
    15181 caacgcctaa aagtgtattt ttcgtttcgt ttgtttttgg aagaaatgga cggctatttt
    15241 cttttttatt gactactttc aatatactgt ccgcctcttt atcctcagaa agaaaagttg
    15301 aaatggtaaa cggaatttct gtttcactta aaatataacc agaaggtgct tcgatctctt
    15361 ttaatcgata acttcctgtt ttcaacccgg aaattgaaaa agcgccattt ttatcggaaa
    15421 tcaggtgaag cgcatctgat tcgttctttg tccacttgta tccgttggct gtttgctgga
    15481 ggtaatttcc tgctttgttt ttaataagga atactgcgcc agctaatcgt ttatctggtt
    15541 tatttgtctc gattttgaca aattgcttac tccctgtata tacagagact ttctttgtac
    15601 tgacaccaaa accaaaatcc agtgttccgt tgttgttgat ttccttgttc gcctgtgctg
    15661 tactgcttag ctgcatctga taagaaatgg tcagtttttt tccagcatat ttttccaacg
    15721 aagggatcga aaataaaaca tcaaatccgt gaggtttctt gtgtatgaca aagccttctg
    15781 tcagcttttc tccattcgat gaaatcgtta aactattagg taaaaacgtt aatgcttcat
    15841 ccgcactatc tgacaattcg aacttttgat agtcaaggat atttacagga attgtcgtat
    15901 gcaaggaata agtgattttt tcaccaatcg taaaatcatt tctaggctct tctaatacct
    15961 tctcaaaagg tggatcaggg tagtcgtttt cttcattttt aggatacaaa tggatagttg
    16021 aaagtttttg attgttttgt ccatgtacag gaagaacaac taccatattc tctgcctttt
    16081 ctttgacgac ttctggtact ttggattcaa tgaaaagata cgctttatct cttttttctt
    16141 gatctttagc ggccagagaa aaagaagcga taccatcttc gttgttaaga gttgtagttg
    16201 ttgcttctgc ggtaaacata ccggaagacg caccgttttt tgcgatctct gcttgtgctt
    16261 cttctaccgt tttgcccttt tcccgtagat ggtaaaaaga atctgtgaca tcataaactt
    16321 ggaatgtgac accatttaat cctcgatacg tttgtaataa agctttttct tgtccgtcat
    16381 ttggatgatt tttcggcagt tgcccgtttg ggaaaagcaa tttgtgtaaa gtaatctgaa
    16441 ccatttctgt ttctgtttct gttgctacct gagtaaaagc tggtttaaat agtaacaaga
    16501 agagacacat actaagccaa ccaagttttt tcatatttag ctgccccctc tttctttttt
    16561 caaaaaaaga taccaaatag aaaacgcaca accaatcatc cctactagat agattcctaa
    16621 acggccaatt cctcccgttt caggtaatgg tacttttgcc tgattcgtga tgtctaaaga
    16681 aatctggttg tttttggctc ctggtgacag aacagattca tgatcttgtc catccacttg
    16741 aattgaccca tcttcgtgta taactatagt aactggctct tttagacctt ggtatccttc
    16801 tggtgtaaaa gtttcggtca gcgtataagt tccaggtttt aaattttcga atagaaaggt
    16861 atctgtttct tgtccatctt ttggcgattc taagtccatt tctggtccct gcagtctgaa
    16921 ttttgctcct tttaatgtct gtccgttgtc ttcttttttg tgtaccgtta gatcaaacgc
    16981 ttttagtcga ttttgatgag tgagcgtcca tacattattt gtcatctcgg tattttttcc
    17041 cgtgtaaatg atttttccat atttatcgat ttggaattca taatttcccg gcgaagtatc
    17101 atatccggca ggaccagctg tttcaacgac tcgatattta ccaggtgtga gtgaatcaaa
    17161 actggcaaga cctttttcat tcgttgtttg gctgtcaatt ggctgataag taccatttgc
    17221 ttcttttttc tgtagggaaa atgctgctcc tttaagattt atctctttgt ccgtcccttg
    17281 catcgcatac ttacgtactg ctacaggaac ttcaacaaaa ggattttcaa tagtcaactg
    17341 gatcgtatca tcggaagtag tgactgtttt tccatcaatg gttaccgtac catcagaagc
    17401 gatcttgatt tcccaagtag tctttttgct taatccatgc ccttctggag cttttgtttc
    17461 tgtcagcgta taggtcattt ctttttgcaa tttgacattt tctggaagag aataggttcc
    17521 gtcgccatga tctgttagta aagtatcaat agaatctcct gttaatttga aaacagcgcc
    17581 aataagatcc ttttcacctg tagaggaatt tttcgttatt ttcaagttta acggtgtgaa
    17641 ttgtttagta tttttccatg tcattgcatc tatttgttga gaatcgtatc ctggtacagg
    17701 taattctttg attgtttggt aactgaatgc ttgaccttga ttattgtatt gaggtagtga
    17761 taatatctct tgataacttt ctccgctatt tgcagataat ttgtcaatgt ctgcacgttc
    17821 ccacgtattt gttgtatctt tagctggttt aatgattcga atatatccgt ttttccaagc
    17881 tgcagcattt gtcgtatgtt cccgttgaat ctcaaaagta acttgatctg gacgatcagc
    17941 tagattgttg tcaaattctt cccataactt tttgatgtga agactgactc cgggagcttt
    18001 agcagatggt ataccgaatt cagctaattc attggtatca atgtttggct ggaaagttgt
    18061 cctgccgttc atttgatacc agaaatttgg atggaagtcc tcattttctg tttggattct
    18121 cacttggtaa tggatttgga tttcttggtc ttttcataaa tagatctgat tgctcttgat
    18181 ggtatttcct tctatggaaa tagatggagt gaccgttgta ggacttgtcc ccacactctt
    18241 gactgaaagt gtaccaggct gataaatgaa tggttctgcg attggatcat ttatttgtcc
    18301 attgcttaca gttgctgaga tctgtacagc ttttttggct aggtattcag agatatctgt
    18361 tgcatgatca gctgattcat agtaaagatc gcctttttca tctgatgaaa ccatttgtcg
    18421 catacgagac tctacttctg cttttgagag accagctgcc ggatcgcttt gaagttggat
    18481 gccaagacca tgtatttcga ttcctcgttc tttgagtgcc atcgcttctc cgatggttgc
    18541 gataaacgta ctgtcgattc ttctggatag attgttttgg tcaggtgcat catagattct
    18601 tgagattaga gaagtatttc ccggccgatc ttgcgtatta gaaaactgag ttccgtaata
    18661 attgctgctt gattgtgcgt gtacccgctg tactttatag gaaaatgttg gtacaccatc
    18721 cgtcagcaaa acgatcactt ttttatgtcc atttggaacg gatagcatgc ttcctgcatc
    18781 tcttagtgct ttttgtgtaa aagtaccacc atttgtccgt gaaggtgtaa tggattttac
    18841 ttgatttttc actgaatcaa atgaacccat ctgtactgca ccgttactgt agctatatcc
    18901 ttcgcttgag tagccgacat atcccatatt gattttgtct gtgataccgc tatctgctaa
    18961 agtatcgaca aaacgatcga caccaatctt tacttcaccg atccgattat tgtcgttcat
    19021 acttcctgac cagtctacga ccaatacgag atcaagaggc gtgatatctt tttgtacatt
    19081 tccacgagca ttcaaataaa catcaaacaa tccgggtgta cttgtttcct ttgcatactt
    19141 tcgtatcgca tagtctgctt gattaccggt tcctccatat tcgatatacg aatgggtccg
    19201 atcattagga tctccattcc aagaatttat accatcccat tgatttgttc cgtttttatt
    19261 gccttgatga tcaagaacat ttgtatttcc tgtaggttgc caactggctt taggataagt
    19321 gccctgatca tctgttgtat attctgtagg aatcgtttga ttagatggca cactcacagc
    19381 tggcaaggat aatgtaggca atgtaaatgg gctgacagaa ttttcgtttt cttttgatga
    19441 ttcttcttct atttcgtctg gctttggtac tgacacatct gtatctttct tattttctgt
    19501 ctcatcaata atgtcatatt ctttattatc gacgatcagc ttgtttgcct ggctgtggta
    19561 aagtgctgga tcgattggaa aactcagatc gatagtttgt ttagtgtttg atgagatttc
    19621 caacgtaatt ttcccgctat ttacctgata agcatagcca tctccttctt ttctttccac
    19681 atcacttaag taattttcga ctagaccaat ttctttttct atcttttcag cactggtgtt
    19741 attcgcattt agcttgatgt tcagtgcttt ttttccagac tgatccgaaa tgacttcagc
    19801 tgaaattgtt tctaaaactg ttggatcatc tgtagtgtct gctattgcag ataatggcac
    19861 aaagtttgat aaactcaata ttatcagcat gaaaataact ttcagtttct tccctgtggt
    19921 cgtcattttt cccctccttt ttcttttttt ctgttttgac gtgaatagtg aaaattatta
    19981 aaataaacag gcgttacata attttattta ttttaatatg ataattatta tacgaagggc
    20041 gctaaaggga atcacaccaa ttttcatacc aattggtttc atttatgaag ttgattcctt
    20101 cgattaatga gcgtttcatt gaaagaaaag ggttgaggga gaaaaacata tgatcaggtt
    20161 gttggaatga aactatagaa agatggaaaa aatcattcga attctgtcca tttctgacaa
    20221 gtggatgact aaaaaagaac ttgccaaacg aattggtagt tcagaatcta catttgtaag
    20281 gtatatcgaa gagatcaaaa tccgctgggg aacggatgta acgattcaat cttcacaaaa
    20341 attggggtat cgtcttgaac atttgaatgt accagcttat ttaaatgttc tgatggaaat
    20401 gtcgcaaagt tctacaaaca gtcgtttatt aaatgagatt attgtcaatc cgggtaaaac
    20461 gattgagtat tattgtgaaa aaatttccat cagccggtct tcttttacac gtaagttgaa
    20521 acaatgcaat caaattttag aaagctattt attaaaagtg atcgttgacc aaggttttca
    20581 gttaactagc cttaaaagtg agctccagtt gcgtatcttt gttacctttt tctttttaag
    20641 ttactacgga agacgcacgt tgccatttcc aatcgaaaaa aagaagatca aagaaattat
    20701 ggtcaagaat aattgtcaga tgaatttgtt tcaggaagaa agcccatatg agcaatcttt
    20761 ctttattatg tattttgttg tgcatctcat gagagaatcc caaggaactc catttgaatg
    20821 gaaagctgga gactcttatg tacagttgat aaatatcgat aaaaaagact tcttaatctt
    20881 gaaaaatctc ttaggaaaat tgaagtacag cagctatata aaagtagtga actattttgt
    20941 caatacgatt ttttcagaat tttcggagaa agaaaaaaat gaaatcagtg agcagatacg
    21001 aaaaaatatg tcctactgtt cgttttcatc cgctgattgc aaagaaaaaa acgaagaata
    21061 tgttgttcaa ttattaacgc aaatttattt tttctcccgt attttcccat acgatacgac
    21121 tcatttgacg atccgtgcaa cttactttgc acagcagatg caacaccgcc agatacgtct
    21181 atatgctgga tttcgtgcag aacttcaatt ttattccact ctactgaatc agaatctgat
    21241 ttattattta tctagctata tcttttggat gcttatcggt aatcctgaaa ttcaccatta
    21301 tacttctgat aaaaaaatac tgattatcag cgacttaagt cttcgacata gtcaatatat
    21361 tgaagaatat atcagtgata ttcttgctgt gcataaaatt cactctgaga caactgctat
    21421 tactgaagat cagctgtcga aatacaatct tctcgagtat gatctgatcg ttacgaatca
    21481 acccattttg aatgcccatg ttcctcatat attgattgat gatagtgtgt cattcgcaaa
    21541 tgaagaagaa ctcatccgct tattcgagtt atgaataatc aaaaaacagc tatgtataaa
    21601 ccaccctgat aaaaatttca ggacaaatac acatagctgt ttctttttat tgtattcctt
    21661 cagcaatcca acgagattta tagcgaaagt cttttaacaa ctcttctttt gtccaattat
    21721 ttgcaatgag atccgtcaaa gtgttcatta cgagggtcat ggaaaagtat aggaaactaa
    21781 ctgcttcttc cgacgattga atggagaaat gattgattct taataattcc agccattttt
    21841 cgttcattgg agaatcagcc gatagttctc tacgtttggt tgtctgctga tcatttcgct
    21901 gggttaacaa gtataacctt tgccaataga tactttcgtg atctaagtcg cagcaccact
    21961 ctaaatattt ttcgatcccc aaaaagaaat gttgtttgtt ttcgctaatg aattttaaga
    22021 tatcttgatg gactatgtga gaactgcttt tgatcgtata caaataagca tcttccaaat
    22081 cttgaaaata tttataaaaa gcaccgcgcg acatccccat cttgtcaacg atttctgaga
    22141 ctttgacttg gctgatgtgc tgtgagcaaa atatctccag taataggtca tcgattctct
    22201 gtttctttgc gttcgataaa ttgaaaaaag tttcttttgg catccacatc tctcctttat
    22261 ctctgattat acgtggatag cccttcattg tcatgaagga ttcattcctt ctttttctct
    22321 tcttctgtca agtacccatc ttccattcga taaacgcgat cactccattg aatcatacgg
    22381 acatcatgag taaccataat agtcgctttt tgtttctcat gagcttcttt aactagtaat
    22441 ttgacgactt catatgcatg atctgtatcc aaactagctg ttggttcgtc tgctaagatc
    22501 aagcttggct cattatataa agcgcgagca attgccactc tctgtctttc tccaccagaa
    22561 aggtctttag gataactatt tttaagtgac tggatatcta aagattgcag caaggaatcg
    22621 atttttgttg aatcttcttt ttttgaagta acttggtcaa ctagttcaaa ttgttccttt
    22681 accgttaaaa aaggaatcaa attagagctt tggagaataa atcctatttc tttaaagcgt
    22741 aaatctgctc gttttttttc tggaagctca gttagtgagt gaccgttgat cactatttct
    22801 ccggaagtcg gtgtttgcaa tcctcctgaa atggtaagga aagtactttt acctgaacca
    22861 gaaggaccaa tgatgctgac gaattctcct tcctttacat aaaaatcaat tgctttcaaa
    22921 gctgagacct ttgcatgacc agaaccaaat gttttggtaa cttttttcat cgttaatatt
    22981 tttttcattt ttattaacct cctattgctg taattgggtc tactttggtt atcgtacgta
    23041 tcgaaaacaa tccgcctagc attgcgacaa tgatcaaaat cccactatat agcaaccatt
    23101 gtgaccaaaa aacagcaaaa ggcatcgcgc ttggtaaaat cagactagtc aaataagcaa
    23161 acaaaagagc aagcagaacg cctaccaccc caacaataaa ggactgtgct actaaagatt
    23221 tagcgatgaa gaaatttttg atcccttgtg ctttcattac tccaaaaata gcagtcttct
    23281 gaagtgtcat cacgtacatg aaaataccga ccacggcagc aaccactaag aacaaaaagt
    23341 atatcatcgc atttaaggtc atattttggg cagaatagcc aggaatactt tcaatgaatg
    23401 tcccaatagt taataactgt agacccttcg atgctttttc tttcgaaatt ttagctgaat
    23461 cctttattac gatcccattg atcggctgtt cttcttcaga agcaaacggc tgttgtccat
    23521 acttcaaagc agtccacgta tctagggacg catagatgac gggagaaact gtgtaataag
    23581 tttcaggaaa gataccgaca atcgtcaaag gatcgtcgta actgccaatt ttgatcgtat
    23641 ctccaatttt atagcctgaa tcagccagat tttgagagat gatgatttca ttctttgttt
    23701 taaattcatg ccctttcgtg agttttggta ataaaaatgc ttctttcgtt gtgccgaaaa
    23761 ctgtaacatt ttctttgttt tttcctttga ttgctccgct gtacaatccg attggtgctt
    23821 tctctcctcc ttcaatgtat ttcagatctc ctcttgttaa ttgtgaagct gcaaatactt
    23881 gatttgcatc ctccgacaaa acaatctctt gtgcgtccca atcatcgatt gcttttttaa
    23941 actcttctga aagtccattc gctagtcctg acaacatgaa tacgacatac gcaatcaaga
    24001 acataatacc gacgatcaag ccatatcgta atttagaata gcgaatttct ttgatagcta
    24061 gaaacatttg tttttcctcc ctatgataga ttgatagtga caaaatgtca cttgttgaat
    24121 agtgtaactc taaagtgaca atttgtcact tgtgttgttt ttttcttatc aataaattgt
    24181 ctaagacatc tttcgataaa accttaacat gagtcataaa aaatttattt aagccttatt
    24241 tatgctttcc atgataaact aaagtgacca aatcaatgag gtgggaaaat gattaaacta
    24301 ttagtagtag aagacgataa agtattatcc aataatataa aagaaatact cgctgcaatc
    24361 ggcgagataa cacaagttta tgatggtagt gaaggactgt tcgagttgga aagtggagtt
    24421 tatgatcttg caatcttgga tttgatgatg ccggaaatga atggatatga agtgttagca
    24481 aaaatacgga aagaaaaaat acagactcca gtattgattt tgactgcaaa agatagtttg
    24541 gaagataaga ttgaaggatt tgaacgaggt gcagatgatt atctaacaaa acctttctat
    24601 cgagaagaac tgatcatgcg cgtgaaagca ttattgaaac gctcgctagg tctctttgat
    24661 gaaaatgttc tgaagtataa ggatatcagt tgtgatttaa aaagcaatga agtcttgtat
    24721 aaagaacaag ttttgcccat acaaggaaaa gagtttgaat tgctcgttta ttttttacaa
    24781 aataagggaa tcattttgac aaaagagcag atatttgacc ggatctgggg atttgattct
    24841 gatacgacga ttacagttat tgaagtttat atgagcaact tacgaaaaca tctgcgaagt
    24901 actggattgg ataaagaaat caaaacctta cgtaatgtcg gttatatact gcaaagggtg
    24961 tgatgcaatg aatggagata ttacacgaaa gcaacgtagt cgcttttttg cgacaaatgt
    25021 agcagcattt gcttttatct tttttctttt gggattcatt actttacaga tattaaacca
    25081 atctgcatac agagaaacag atctgtcatt agagaaaata aattttgatt ctcgaacagt
    25141 acaattagag atacaaagat atcagcaagg cgatcccttt ttaaacccga ctcaaacgga
    25201 acaaaacaga acaaacatgg aacagccatt aatggaacca tcaaatgacc gttttaacac
    25261 tcaagtgatc ttgtggtcta aaaatggtga gatattgaat aaagcagcta ttggtgggcg
    25321 tttcaaccaa atcaaagaat taaaaatgga tacgaaaaat ctgaatacca tccaaaatat
    25381 tgaattaaat gaagacagtg gctcatctgc cctctctttc cgttcgatta cgaaaaaagc
    25441 ggagacgaat caagatggcg ttgcctacat ccaaatttta gaaaatacaa atcaaatcaa
    25501 gaattcatta gcgacttttc gcacgattat gattttgtgt atgattgttt tttggattct
    25561 ctctattggt atcagttatt acttgtctag tctaagcatg cgccctatta ttctttcttg
    25621 gagaagacaa aaagaatttg ttgagaatgc ttctcatgaa ttgcgcacac cgttaacgat
    25681 tattcaaaat agtttggaac atctttttac caagcctaat catactatta ttgaagaatc
    25741 cgaaagcatc gctcaagcat tgtcagagac gaggcgcctc actggattga cttcggattt
    25801 gctgacaatc gctcggaatg attctgatca gcagctgctt tcaaagcaaa tgatcaatac
    25861 acaagaatat atcgaacaac ttgttaaacc atttcaagag atggccatca tagatggaaa
    25921 agagtttatt ctccataacg atgcaacatt agaagtacca attgatgaaa aaaaaatcca
    25981 tcaagttttg gttattttgc tggataatgc attaaaatat acgaatccag aagataagat
    26041 cattttggat tcatctgtta accataaaga atggctgatt cacgtaaaga atactggccc
    26101 ttctatcagt gaagaagatc aagcaaggat ttttgatcga ttttatcggg aagatcaatc
    26161 tcgggcgaaa gagactggcg gatacgggct tggcttggca attgcaaaac aggtaatcga
    26221 acagcatcat ggacagattt ctgtatccaa ttatcttcca caaggagtcg attttaaagt
    26281 ccaattgccg ttgaagccta aaaaataatg cttatcccta aaaaagggct gtgacaaaaa
    26341 tcttgtcaca gccctttttt gttgcgaata aacggtgttc cagaagtagc ttcttcggaa
    26401 ataagccgaa atttctttaa gctgaccact tctgtcacaa cctctttagc ttacgcttac
    26461 gattttttat tgcggtcttc aatgtgtaca tcaccgcttg tgtcaatatc caattcttct
    26521 cggctaacat cttctgtcac tttttcaaca tcttcatgtt cttctttatg aatcccaact
    26581 tcttcacgaa taactgtatg tttgcttaca tctacttgct cttctttgat tgggatagta
    26641 atcgtgtcat cttccaattt ttcgtctgtt ccctgtgaag attgatctgt tactggtttg
    26701 cgttcgatga cgatttcttc acgtttgacc ggtacttcta ccgtttcagt atcgttgacc
    26761 acatgtttgt ggatgtcgac ttctccagtt gtcacatcat gtgtatttac atctaatttt
    26821 tcttctttta gttgaattgt atcatcatct agatttggtt tgcgatcgat gtcttcagtc
    26881 ttgcttgtac ccattgtatc agtctcgcca ggtacgtcca tcggatcagt tcctggtgtc
    26941 atattcaatg gatccgttgt attttgaaag cctgtcgtgc cacctacgtc cattggatct
    27001 gttccagtgc cagtttctaa tccagtcccc attgtgcttg ctgggctaac agtatcttgt
    27061 gtatccattg tatcttctgt tgtttctggc tgacgatagc cttttactgt gatgacaaat
    27121 tttccatcag aaatatctcc tctatattga tataaaggat cattttctgg tgtagccgtc
    27181 tcagaatcat aggtatcaaa agaaaaagta tcttttattt tttcccataa agaacggtct
    27241 tcttcaccat ccatccgatt ttcatctgtt tcaacatcta taccagacaa tccgtcaaat
    27301 cctagtctag atgctgcttc atcagtagta taaataacga tatcatctcg ttcataccct
    27361 tcgttacgca accgttcaat tactgataat gcttcttccc tcgtactata gcttccgatg
    27421 accgttaaat ttttctcttc catgtaaatt cctcctagca aat
//
LOCUS       DAZKLD010000021        26493 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.021, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000021 DAZKLD010000000
VERSION     DAZKLD010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26493
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.021"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            230..2065
                     /gene="tdc"
                     /locus_tag="ACG8KR_000764"
     CDS             230..2065
                     /gene="tdc"
                     /locus_tag="ACG8KR_000764"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004837 - tyrosine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HFQ0200161.1"
                     /translation="MKDMDIKAVFIGDKAENGPVYKMLLNKMVDEHLGWRENYIPSDM
                     PAISEGDKLTPDYLATRDHMIEVLDEVSQRLRAGSIPWHSAGRYWGQMNAETLMPALL
                     AYNYAMLWNPNNVALESSMATSQMEAEVGQDFASLFNMTDGWGHIAADGSIANLEGLW
                     YARCIKSIPLAVKEVLPEKVKKMSEWELLNLSVEEILEMTESFTDEEMDEVKAASSRS
                     GKNIQRLGKWLVPQTKHYSWMKALDICGVGLDQMVAIPVQEDYRMDINALEKTIRELA
                     GQKIPILGVVAVVGTTEEGQVDSVDKIVQLRERLKDEGIYFYLHVDAAYGGYARSLFL
                     NEAGEFVPYASLAEFFEEHHVFHHCVTIDKEVYEGFRAISEADSVTIDPHKMGYVPYA
                     AGGIVIKHKNMRNIISYFAPYVFEKSVKAPDMLGAYILEGSKAGATAAAVWTAHRVLP
                     LNVTGYGQLIGASIEAAQRFREFLDHLTFTVKGKTIEVYPLNHPDFNMVNWVFKEQGC
                     TDLNAINELNEKMFDRSSYMDGDVYGERFITSHTTFTQEDYGDSPIRFVERMGLTKEE
                     WKKEQKITLLRAAIMTPYLNDDRIFNFYTKKIAKAMEKKLNEIIQ"
     gene            2370..3848
                     /locus_tag="ACG8KR_000765"
     CDS             2370..3848
                     /locus_tag="ACG8KR_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFQ0200162.1"
                     /translation="MKDTGSSKQITTMTFIGMTCALVASVRNIPDVAATSWTMIFYML
                     VAVLLYAFPISLISGEFAGMFPQKGGPELWVSNALGKKWGFVVSWLLWVQMFPGMVMV
                     ASALAPLFGNIIDNVPLGLNSKFTLVVILVVYWIITFLNLKFDMAKIGGKVGVWLGLY
                     VPLTMMLLLGFAAWIKVGIVPTGTLGSFSWDKLIPDTTTASSFVYFAPIMFIFTGIEM
                     SSVYITRLENPVKTYIRGVFAALIFIFFVNILNALVVANVVPKGQMELNNIAQSISIY
                     CQILGLPHLIVNLFSLLVFIGVAVQLSAWASGPAKTVTESARKGAYPPKFNFWKTNQF
                     DVSKSVILTQSVIISIFALFYLLIPGVNQAFLMLVNSTTVIYCIVYVIMGIAVLRLRY
                     THAKLNRPFRIGKKEKKSNLGVWTVVIVLFAAIGFSVGLTMKAGTWINLIAVTAISVI
                     LFVVPLCIEKIKKPSWEQEVAMLLEKEKGELDGKTGNETDSN"
     gene            3814..5145
                     /locus_tag="ACG8KR_000766"
     CDS             3814..5145
                     /locus_tag="ACG8KR_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFQ0200163.1"
                     /translation="MEKPAMKRTLTNRNIQLIALGGAIGTGLFLGSAKAIASAGPAVI
                     LVYLICGLAIYGLMRAMGDLFLANSTFNSISDFVDYYLGKKWAFFVNWTYWLCWLGAG
                     LAELTAIGLYIHFWFVHVPALISGGSALMILMAVNLVAVKWFGELESFFSIIKILGIF
                     LFILLGIGLSVSDFHTQSIGIKENFGQIDELLPFGFAGMVVSFPMVLFSFAGIEMIGL
                     TVGETSDPQKNLKKAINCLPWRILFFYIGTILSILFVVPWHCLDLKESPLVTMLQVIH
                     CQGGAAIVNLMILVASLSSANSAVYSTSRILYQTAKEKSLPHWFCALSKKGVPLHGIL
                     ITGMLFFAGLCLHFFVADSRLFFQLLAGMTTSCFLFVWSSILCAHVQFIKEKKRNTTQ
                     RYKDLALLVFFTSIAFSLLFESTTRFIPFLLVIWFVFLNFVYKRISNRNIS"
     gene            5259..7811
                     /locus_tag="ACG8KR_000767"
     CDS             5259..7811
                     /locus_tag="ACG8KR_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HFQ0200164.1"
                     /translation="MNWYRLTVEDVLKNYQTSTEGLSQEERQKRLTNQGYNEIKARQQ
                     TKRWKKIAKHFTDLLMIVLIIASFLKFASSEYIEGSIILFVVIVNGLVSYWQERKAEE
                     SLNGLKQLMGQEAIVLSNGMQEKIPAKELVKGDVIVLRAGDVVPADIRLIEAHDLLVE
                     ESILTGESEASEKSHAVLTEEESIGDQKNMGFSGTLVQSGSAVGVVVETGNTTEIGKI
                     NQALQSVEQQTTPLIKKINRLNKQIFQGILCLSLFLVIFTTFRYGMDWHILLSATIAL
                     VVSMVPEGLPAVLTMILSVGVHEMAKEKAIIKGLPSVETLGSMTVICSDKTGTLTKNE
                     MTVVDVAAKEEACVLSIMKNCQELKTKEEQKTENLSGNPTEVALIRHTETANLPLKKI
                     EAKIPFSSEYKYMATMHAEEKGAVIYVKGAPEVLFAKSTLSSAEQEAWSQTAAEFARK
                     GQRVLGFAYKKVDSKQELTHETLTQLTFAGIAGLIDPPKESAVKAVKECQQAGISVKM
                     ITGDHKDTAKAIAEQIGLKHTAKVLEGIDLDLMSDEELIQQVPIVDVFARTTPEHKLR
                     IVKALQKNGEIVGMTGDGVNDAPALKRSDVGIAMGIKGSEVSKQAADMVLGDDNFHTI
                     AKAVKEGRRILDNLQKTINFFLPTALAQGLILIWALMLNRPLPLSPVQILWVNMVTTI
                     TLSYALGFESPDPEIMKRPPRDPKQGILSGYHLFRIIYVSLLIMVPAYLLAMQFEGQS
                     LQQTILLQNIVLAQAVYMINCRELSKPSLNQGLLQNKALFLSLGILALLQGMVIFLPV
                     GQQLIGTVSLTMTQQLLIGANVILLFLVVEIEKGIMNKLFRKKEKTAREYSR"
     gene            complement(7818..9257)
                     /locus_tag="ACG8KR_000768"
     CDS             complement(7818..9257)
                     /locus_tag="ACG8KR_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0200165.1"
                     /translation="MYSLMKKIITEKDIRRHVSLVEQLLNHTKITVNELAEIIGTTER
                     TIFSDLQSIRSQLPEGWDIISDQAGISLQNQQNLLTNDLWEIFFKQSVSVELLKNLLF
                     TKKVAVPDFLADHGLSYGTLKRHVTKINQRLASYDLQIDLTKYTACILGKERAIRTFY
                     HRLLIPFTHNNYFFEDYSIHESHYFQFLRNLSQTELAVETEEIFGTCWFFINTIRIKA
                     NCRLDSSIHTNSTLSSLYDSALKKLYLTEGIYLKDTELSFASFCFLESWNYNNNYGQE
                     TARCLHHSPFLEVLETFVEELASELSLDQLKKTSLTDNLALLILKYHESPILIEALDR
                     QYHRFLETYDQQYAHLYQQKENLLNDLKKEIEINEPNYFLQLLSLLIQQTIFSIKPNL
                     FNVYFIFQGEPSWKAFLQQELKDYLGKRVCFLPIELTDLSTISFEKTDILISNFPLDS
                     LDIPIVYISSIPTKNELNRLTELTFKTFL"
     gene            9232..9414
                     /locus_tag="ACG8KR_000769"
     CDS             9232..9414
                     /locus_tag="ACG8KR_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0200166.1"
                     /translation="MIFFISEYINQTPFYSKNTEKINGGQVSTDFSIIVYFSGAFTDD
                     KNFIMNLKNDTTKIKK"
     gene            9603..9965
                     /locus_tag="ACG8KR_000770"
     CDS             9603..9965
                     /locus_tag="ACG8KR_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HFQ0200167.1"
                     /translation="MLTKKELIDHINETYNLSPEYIFKKFPDYCVFRHQKSKKWFGVL
                     MSVPEEKIYDDASDHRIINILDLKVDPELGGILRGKEGYFPAYHMNKEHWISIDLRKI
                     EKLDELADLIDGSFKSTQ"
     gene            complement(10152..11009)
                     /locus_tag="ACG8KR_000771"
     CDS             complement(10152..11009)
                     /locus_tag="ACG8KR_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFQ0200168.1"
                     /translation="MKLLHLQYFKKVFETKNVTQAAKELFISQPALSRAIKHLENELG
                     VPLFYHSGRNIETTVYAEEFYPYAVKTLDTLEEGIKLLSSVNEKIVTSVILYLEVASV
                     SIPNLVRIFMEKHPDIQLTIMQHHLPDDITDPVLYITSEMKPGMTNIPIMKEPVYVAI
                     PKKHPLAKKEHLQLEDIQRTPILMLSKSNAFRHTLDAALEAKSIELTISSTTDDPATL
                     RSIVKQGLAVSFFPKVSWSYDKNDPFVLREIKDLPLTRTIYLSSSFTEDHPLIKMIAK
                     TMREFYLGK"
     gene            11129..12040
                     /locus_tag="ACG8KR_000772"
     CDS             11129..12040
                     /locus_tag="ACG8KR_000772"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFQ0200169.1"
                     /translation="MSTGFLFPGQGSQKLGMLADIPEMYMERLENVTGYRLVDKEENY
                     QDTVFIQLALLTKAVFYLDEMEKNGIQPDIAAGHSIGAFCAAVACRSLRFENAAELVY
                     HRAVLMKEAYPSGYAMGVVVGVTRSEAEEIVAKTFDPDHPVYLSNENCPMQHTLSGSI
                     EGLSVTLAEAKRHQAQTAKLLKVPVPSHCLLMNDTVKKFKPYMQSVHLSDARCLYIKN
                     TDGRATQDAEEIRKDLLENLAFPVQWNQMMDLAKELGMDLTIEFPPGNTLTKLIHTKF
                     GVDKGIRTINLDQHGIDDATFLYQKWR"
     gene            12047..13693
                     /gene="mdcA"
                     /locus_tag="ACG8KR_000773"
     CDS             12047..13693
                     /gene="mdcA"
                     /locus_tag="ACG8KR_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015451 - decarboxylation-driven active
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit alpha"
                     /protein_id="HFQ0200170.1"
                     /translation="MEKPNRNWASKREKKAQRLEKISNLLNGKYVETEKIVEVMEKLI
                     APGDKVVLEGDNQKQASFLSESLEKVDAEKVNDLHLIMSSISRPEHLNIFEKGIASKI
                     DFSYAGAQSLRVAQMIEDGTMKLGDIHTYLELYGRLFIDLIPNVVLVAADKADKDGNL
                     YTGFNTEETPTIIEAAAFKDGIVIVQVNEVVDSLPRVDIPGDWVDVVVKADKPYQLEA
                     LFTRDPQNITELQILMAMMAIKGIYAEHGVQSLNHGIGFNTAAIELLLPTYGESLGLK
                     GKIAKNWVLNPHPTMIPAIESGWVESIHSFGGEVGMEKYIAARPDVFFVGKDGTMRSN
                     RTLSQAAGQYAIDMFIGSTLQLDYEGNSSTVTKGRLSGFGGAPNMGHNPGGRRHSTPA
                     WQSLRDNDDPLGKGQKLVVQMVETYGSNKKPVFVEELDALAVQKQAGLANAPVMIYSE
                     DTTHIVTEEGIAYLYKAKTKEERQAAIAAIGGVTPLGMTSTKESLDSLRAAGIVKLPE
                     DLGIKRSEAKRSLLAAQTIDDLIEWSDGLYEPPMKFRTWS"
     gene            13795..14094
                     /locus_tag="ACG8KR_000774"
     CDS             13795..14094
                     /locus_tag="ACG8KR_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit delta"
                     /protein_id="HFQ0200171.1"
                     /translation="MEQLAYKYPTKQPINKKIHVGVVGSGDLEILMFPTEKTESTVKI
                     CTGSDGFGEVWNNVLTRFFDRYPISADIVINDFGATPGVVYLRLTQAMEELSDEK"
     gene            14084..15760
                     /gene="mdcD"
                     /locus_tag="ACG8KR_000775"
     CDS             14084..15760
                     /gene="mdcD"
                     /locus_tag="ACG8KR_000775"
                     /EC_number="4.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-independent malonate decarboxylase
                     subunit beta"
                     /protein_id="HFQ0200172.1"
                     /translation="MKNSFVELNARERVNALLDNGRELIDPFDQIIAPSLPAQGIVPE
                     SDDGIVVSRGTINGKQAVVIAMEGKFQGGGIGEVSGAKIVAALEHVLEENKKGNEIFP
                     VIVLDTGGVRLQEANYGLLSISEIGNVIIALKQYVPVIGLIPGRVGSFGGMSITSALM
                     SYLIATKKARVGLNGPEVIEQEAGVREFDSSDKDLIWNTLGSRQRVESKIIDELVTDS
                     VEAIKAAVIAAMDERKDTHRSESSAFYLSLLDSLDLSKPMAIDDYNEAIANHQAKSID
                     LPEVAEGNEPAAASVGYDWFAALTGMKNPTSSISTVYYGKSSKFIEEAIVTSIVPDPK
                     NPMYRVRNGEVGLVEGFRMGQILDHVYEEDKDKKVKRPIIVVIDVPSQAYGYNEELIG
                     IHVALANSAAAYAKLRQAGHPVIGLIAGNAISGAFLAHGLQSSRLIALNNKEITVQAM
                     SKESAARVTKRSVAEIEAAAEKVPAIAYDINNYTKLGAIYHFLEDITDQAASEQNVSK
                     VIEAINEAIQDVRNTNDTMLTNRYTNETAKTFGRVETNKVRAKMDEEWDA"
     gene            15764..16357
                     /locus_tag="ACG8KR_000776"
     CDS             15764..16357
                     /locus_tag="ACG8KR_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase holo-ACP synthase"
                     /protein_id="HFQ0200173.1"
                     /translation="MIQAHDIVLFDKHEIEGFTLPEWVVLTEKMFGTVRRASTDHAEI
                     LPIGLRGPARNQRWSTEISKHAVKQVIHPWDIIEQESYRQEKIMDYPVYQQFHAARNI
                     LSGCKWGIGGSLGFELSTGIPAVKETSDFDLLLYANSPIELPIQVIQSHPAFFEQFDT
                     QVVTRQGGFSLKEYLREPEKKILLKTVEGPKLTKELW"
     gene            16381..17457
                     /locus_tag="ACG8KR_000777"
     CDS             16381..17457
                     /locus_tag="ACG8KR_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFQ0200174.1"
                     /translation="MFENKKIKINVSDFQKVSAVTAVMMQTILSFALANSHSATNTAF
                     LGTFYVIVKYTAPMFIFAIVYNMVKTSQHLSYWEFLKEKFFELVVPYLLWTTAYLWLF
                     PAVQQQTPYTDTASFLLKYVTGDGAPHLWYTVMMLQIQLFMPFFVWLGYKVFANKKYV
                     WPVLIGSTILYVAWYLFYQTQVLTGPYHDSWYLLDRFVASFMIYGVAAFVYHEKVYQY
                     LDRVRYLFLPVALVIAFFSVRSLLAHPGDLSFANAPYLNTIQSLYSLVIIFAVFIGAS
                     KMIVNDSPKLPLFKWLSVYAYRTYLANVFVFQVLLLLFKDSWLQLPSGVMILVAYLMT
                     ASCAFALSWLLHIIWVAIKKGFSK"
     gene            17482..18351
                     /gene="mdcB"
                     /locus_tag="ACG8KR_000778"
     CDS             17482..18351
                     /gene="mdcB"
                     /locus_tag="ACG8KR_000778"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046917 - triphosphoribosyl-dephospho-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0019361 -
                     2'-(5''-triphosphoribosyl)-3'-dephospho-CoA biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase MdcB"
                     /protein_id="HFQ0200175.1"
                     /translation="MINDERKKAVTQQLAALVEEALIAEVTLSPKPGLVDALDAGAHS
                     DMDYALFLKSAKTLTPFFEEMAQIAWHHAIDQELRERIAEIGRKAEKAMLIATNGINT
                     HKGAIWAMGLLISVIAQKISMEQPFSLSKILDDVSKLAAFPDTKYRHKKTTHGHRVKQ
                     KYGVNGAYEEAILGYPHIRLAIQAAKELDNDSYVQKQMHMLLVLMSSLDDTCVLHRSD
                     QKTLVEMQKLAHAASQSALPNRPFTALLHFCQENQISPGGSADLLSASLFMLKIEELW
                     TADTYICPMLLND"
     gene            18462..19226
                     /locus_tag="ACG8KR_000779"
     CDS             18462..19226
                     /locus_tag="ACG8KR_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HFQ0200176.1"
                     /translation="MKKINYLAIILLVGLCLFALPTQGFAVAGGHGGGTSSGRSSGRG
                     SFGGRSYGGSYFGSRSYGRDSYGYYGPGTGPSSYEQTGFLALAVLSVGGVSAFSIVKN
                     KRQKKRLKAEILSRMSGTAKEKKAQFLEIQKIFMKIQSAWEEETPEKARSCYTDKLFA
                     EHQKVLQKNKADGVRNHTKKITIQEVGNYRQIHENSFAIRIDFRCCDYSVDRKDGRLI
                     SGSKTQRQYFSQIWYFDFSEKEKRWQADFIQPIMLD"
     gene            19715..21808
                     /locus_tag="ACG8KR_000780"
     CDS             19715..21808
                     /locus_tag="ACG8KR_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFQ0200177.1"
                     /translation="MKTRTIEILHRIMTAQYPVKAKHLASEFNVSQRTIRQEVLEANI
                     WLSSKKLPEIRTIRNKGFLLKLEKEKQARLETEIKDVKDGFLNRRERIFDLVLAVAYS
                     RIPIHLNKKEEEFQISKSTLDEDMRRLRATLVSYGIEIVSYGKQGLVYKGSERSIRTM
                     IYDFINQNLGMTDFFMNQDAQLTPAQKIFLHYFPLVEIKKIQEIYSNKLLKQEDDIYK
                     KQILLFTLIWIQRIRKHEFISAINWKNTEIKDSSFSAFIDHMIEEFQLDNVPQVERNY
                     LRFTIETFNARDISNSIEWVQAQLLTIQLIQFVENETHVPFHLKEEALFENLYKHMAA
                     LIVRIKNHIQVMNPLKENIRTNYFPIYRAVTHFVPTIEEVIGGKILEDEIAFLVIHFS
                     TIASVIKRDLNYVYKSVVVCNHGMATGNLLAENLKEKFPQIEITAVLSSKEIELVDKL
                     DVNLVFSTFQLNYRNKPLLVVDPILTETNILVIEDFLKRNEELQRLEPNSHESTELFT
                     QIIDVMKESGGRVDRIIYGKLEKLFETNNLEINKREIQPMLKDILTDSNIMIKEKATT
                     WEESIELAARPLVKENIVEERYVEAMIRAVNDYGPYIVIGKHMALAHARPEDGVNKLG
                     VSVATIEQPIDFGNPEMDPVKIIFCLAAVDSYSHLNIMKELIELINNEEKLDRLIECT
                     NIEDFKQLLFSVVEK"
     gene            21833..22135
                     /locus_tag="ACG8KR_000781"
     CDS             21833..22135
                     /locus_tag="ACG8KR_000781"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFQ0200178.1"
                     /translation="MNKLNILFVCGAGLGSSFAAQMSAEDVLNKLGVEAKLDHCDISS
                     AVSMKPDVIITAENFRSQFEKFAVDPKTAMIYLKNIVSKNEIEEKITPVLQDKGVI"
     gene            22162..23451
                     /locus_tag="ACG8KR_000782"
     CDS             22162..23451
                     /locus_tag="ACG8KR_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFQ0200179.1"
                     /translation="MGVINFIIENILTQASITIALIAMLGLLLQKKSAGQVISGTLKT
                     LLGFQVLSAGSSIIVGSLTYFGKIFTEGFHMQGIIPSIESINGQAMNDLGLGRDIALT
                     FLAIFVFNIILARFTKWKYIFLTGQAILWMATMTTVFGYFAGLRGIVLILVGGFIGAC
                     FAIAMPAVAQPIIRKITGSNDIALGHFCTIGYLFEAGVAKLFGEKGENKKSIEDIKLP
                     THFEFLQDTYLSVMVVMVPLYIITVLFAGEPFASELSGDQNYIMFAFLQAIQFVVGVY
                     VLLAGVRLLLGEIVPAFRGIAMKLVPDAIPALDCPVFFPYSPNAVILGFITTTIGTII
                     AMFTLPMFGLAMILPGMLTNFFAGGTAGIFGNAVGGRRGAIIGGIAHGFFITLLPALL
                     VTIFNSMGFNNATATDVDTVAAALLYAWILSPVLKAF"
     gene            23470..23877
                     /locus_tag="ACG8KR_000783"
     CDS             23470..23877
                     /locus_tag="ACG8KR_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFQ0200180.1"
                     /translation="MFGFFKKKEKEPKKVLAKEEKALSPEATEKIQSEITQLKQEIST
                     TQDKHKLAKLYEQVGLKFSELYVNDQAIQYLEKSLENKQTIGDGYKKLMSLYNQKRAD
                     AARAGDDQGIDYYMGKMDEMRQIAKQVTIKGNK"
     gene            23900..24769
                     /locus_tag="ACG8KR_000784"
     CDS             23900..24769
                     /locus_tag="ACG8KR_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HFQ0200181.1"
                     /translation="MYTTLKEVTKTAEELNFTVGAFNTHNLEMLPAMLGAAKEMGSPI
                     IIQTSVDTAKYIGMHVLVNVMTAIAEEEMVDAVLHLDHARDFDDIKEAIDSGYTSVMY
                     DGSHLPFKENILKTKAVVEYAHAHGVSVEGELGTIGGTEEGIHVAENDKVYTRPEDAV
                     KFVEATGVDALAIAIGTNHGQFKSKTEVNIPLLKEIDAVVDVPLVIHGGTGVKEEDYP
                     ELINNGIRKFNVGTELLVNWTKVAKETFSETEINKSLRHNVIPANEAVKEIVKHKIGL
                     FMNLESPINLGNK"
     gene            24771..25391
                     /locus_tag="ACG8KR_000785"
     CDS             24771..25391
                     /locus_tag="ACG8KR_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFQ0200182.1"
                     /translation="MKKYFILLAGSPGTGKTYLMNKLRERFPDMYAITLDEIKEYYAE
                     SIGFNNLEERAEQERTKVYPFYYKALDLYMEAGKKVIVSEYPFSDKQKARLQELADRY
                     GYQVITIRLTADFEVLWQRRYKRDRESDRHLSYIMDHYHYGDVLEDRTKGTNHITKEA
                     FKKIIADRKYDKFQLGTLYEFDVTDYTQVDYTPLLDKLEKEINDNK"
     gene            25841..26446
                     /locus_tag="ACG8KR_000786"
     CDS             25841..26446
                     /locus_tag="ACG8KR_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HFQ0200183.1"
                     /translation="MNKKLAQYKRLLALSQAGLYYGKDVFDKERFEEIRTISLELISE
                     IGKETFEEIKALTTIGEGHPTPKVDVRAYIKKDGKVLLIEDKRTKEWSLPGGFAEIGL
                     SPEENVRKEVYEETGLTVETTQLRAVFDTNKQKDIPQLFQYYKLVFACAIHGEEAKFI
                     ENNETSNMGFFSIEELPKLSEKRTTKKQLLILENKNQIYCD"
BASE COUNT         8615 a         4525 c         5660 g         7693 t
ORIGIN      
        1 ctttttttag gttatctatt ttagaagact gatttattga aaaattgttc gcttttttgc
       61 gttgaattat cagaaaaatc cgactttatt tatttttgtt tgtaaaaatc tacgtggatt
      121 tgatgttata aaaaaaaaat tctcattttt ggcgctatag tgaagaggtg ttacagataa
      181 acacatgtat ctgcaagaca ttgaccaaca aaatatggag ggaagaaaaa tgaaagatat
      241 ggatatcaag gccgtcttta taggcgacaa agctgaaaat ggaccagttt ataaaatgtt
      301 gttgaacaag atggtagatg aacatcttgg atggagagaa aattatattc cttcagatat
      361 gcctgcaatt agcgaaggag ataagctcac tccagattat ctagccaccc gcgaccatat
      421 gatagaggtc ttggatgaag tcagtcagcg attacgcgca ggatcgatcc catggcattc
      481 agcaggccgt tactggggac aaatgaatgc tgaaacattg atgcctgctt tattagctta
      541 caactatgcg atgctgtgga acccaaataa tgttgcattg gaatcttcta tggcaacttc
      601 gcaaatggaa gcagaagtag gacaggattt tgcttcatta ttcaatatga cagacggatg
      661 gggacacatt gcagcagatg gttctattgc taatcttgaa ggattatggt acgcccgttg
      721 catcaaatcg attcctttag ctgtcaagga agtgctgcct gaaaaagtga aaaaaatgtc
      781 tgagtgggaa cttttgaatt tatctgttga agaaattttg gaaatgaccg aaagctttac
      841 ggacgaagaa atggatgagg tcaaagcagc ttcttcccgt agcggaaaaa atatccaaag
      901 attagggaaa tggctggtcc ctcaaacaaa acactattct tggatgaagg cgttagatat
      961 ttgcggagtc ggcttagatc agatggttgc tatcccagtt caagaggatt atcgaatgga
     1021 tattaatgct ttggaaaaaa caattcgtga attagctgga caaaaaatcc caattctagg
     1081 tgtggttgct gtcgtaggaa cgacagaaga aggtcaagta gacagtgtag ataaaatcgt
     1141 ccaattaaga gaaaggttaa aagatgaagg gatctatttc tatttacatg ttgatgccgc
     1201 atatggcggc tatgcgcgtt cactgtttct aaacgaagca ggagaatttg tgccgtatgc
     1261 ttccttagct gaattctttg aagaacatca tgttttccac cactgcgtaa cgattgacaa
     1321 agaggtatat gaagggttca gagctatttc agaagcggat tctgtcacga tagatcctca
     1381 taagatgggc tatgtccctt atgctgctgg aggaatcgta atcaaacata aaaatatgag
     1441 aaatatcatt tcttatttcg cgccatatgt gtttgaaaaa tcagtaaagg ctccagatat
     1501 gttaggagct tatattttgg aaggatcaaa agctggagca accgccgccg cagtctggac
     1561 agcacatcgt gtattgccat tgaatgtgac cggatatggt caattgattg gggcttcaat
     1621 cgaggcagct caaagattca gagaatttct cgatcatctg acttttactg ttaaaggaaa
     1681 aacaatcgaa gtctatccat tgaatcatcc tgattttaat atggtcaatt gggtattcaa
     1741 agaacaaggc tgtacagatt tgaacgctat caatgaatta aatgaaaaaa tgttcgatcg
     1801 gtcttcttat atggatggcg atgtttatgg tgaacggttt attacttctc atactacatt
     1861 tacacaagaa gattacggtg attctccgat tcgttttgtt gaaagaatgg gtctaacgaa
     1921 agaagaatgg aaaaaagaac agaaaatcac tctgctacgt gcggctatca tgactcctta
     1981 tttgaatgat gatcgaattt tcaattttta tacaaaaaaa atcgccaaag caatggaaaa
     2041 gaaactaaac gaaatcatcc aatagaactg atttttgtgt gaaagaataa caagagacat
     2101 atggagaaca gacaattatt tgcttcatca aaacaattgt atttccccct gtttttcata
     2161 tattcctcgc caagatattc ttttttataa aacgttctat aagttggatc gttcgtttca
     2221 tgtatcaaac ccagttcact aagatcgttc aatggatgtt tcggtgatac tgggttttgt
     2281 actgaatcga tgaaaccttg tcatttctgt tcttgaacaa aaataaaaga aaacaaaaac
     2341 atatgtcgct attgaaagaa ggaataaaga tgaaagatac tggttcatca aagcaaatca
     2401 cgaccatgac ctttatcgga atgacctgcg cccttgtagc aagtgtacga aatattccag
     2461 atgtagcagc aacgagctgg acgatgattt tttacatgct ggttgcagta ttattgtacg
     2521 cttttccaat ctcattgatc tctggtgaat ttgctggaat gtttccccaa aaaggtggtc
     2581 cagaactttg ggtatcgaat gctctaggaa aaaaatgggg gttcgttgtc tcatggctgt
     2641 tatgggtcca aatgtttcct gggatggtga tggtcgcttc agcactcgct ccgttgtttg
     2701 gtaatattat cgataatgtg ccgcttggtt tgaacagcaa atttacatta gttgtcattt
     2761 tagtcgttta ttggatcatc acttttttga atttgaaatt tgatatggca aaaatcggcg
     2821 gaaaagtcgg tgtttggtta ggattgtacg tgcctttgac gatgatgctc ttattagggt
     2881 ttgctgcgtg gatcaaagtt gggatcgtac caaccggaac attaggcagt ttttcatggg
     2941 acaaattaat accagatact acgactgcat cctcatttgt ttattttgcg ccgatcatgt
     3001 ttatttttac tggtatcgaa atgtcctctg tgtatatcac tcgattggaa aatccagtta
     3061 aaacgtatat tcgcggagta tttgccgcgt taatttttat atttttcgtc aatattttga
     3121 atgctttagt tgtagcaaat gtcgtaccaa aaggacaaat ggaattgaat aatatcgcgc
     3181 aatctatttc gatttattgc caaattcttg gtttgccaca tcttattgtg aatctgttta
     3241 gtttacttgt cttcattggt gtagcagtcc aattatctgc ttgggcctca ggaccagcaa
     3301 agacagtgac agaaagtgct agaaaaggtg cgtatccacc aaaattcaat ttttggaaga
     3361 cgaatcaatt tgacgtatcc aaatccgtta ttttgactca gtctgtcatt atctcaatct
     3421 ttgcactttt ttatttgctg attcctggcg tcaatcaagc atttttgatg ctcgtaaatt
     3481 caactactgt tatctactgt atcgtctatg tcatcatggg gatcgcagtt ttgagattgc
     3541 gttatacaca tgcaaagttg aatcgaccat ttagaattgg aaaaaaagag aaaaagagca
     3601 atcttggagt ttggactgtc gttattgttt tatttgcggc aatcggattt tctgtcggac
     3661 tgacgatgaa agctggaaca tggatcaatc taattgcagt cacagccatc agtgtcattt
     3721 tgtttgtagt accactctgt attgaaaaaa taaagaaacc ttcttgggaa caggaagtcg
     3781 ccatgctgct tgaaaaagaa aaaggtgaac ttgatggaaa aaccggcaat gaaacggact
     3841 ctaactaatc gaaatatcca gttaatcgca ttaggtggcg caattggaac aggattattt
     3901 ttaggttcag caaaggcaat tgcatcagca gggccagctg ttattctcgt ttatttgata
     3961 tgcggtttgg ctatttatgg tttgatgcgg gccatgggtg atttattttt agcaaatagt
     4021 acatttaact ctatcagtga tttcgttgac tattatttag gcaaaaagtg ggcatttttt
     4081 gtcaattgga cttattggct ttgctggctt ggagctggac ttgcggaatt aacagcaata
     4141 ggattgtata tccatttctg gtttgtacat gtaccagctt tgataagcgg aggtagcgca
     4201 ctaatgatct taatggcagt caaccttgtt gcagtcaagt ggtttggtga attggaatct
     4261 tttttctcaa tcatcaagat cttggggatt ttccttttta tcttattggg tataggattg
     4321 agtgtctcag attttcatac ccagtctatc ggaatcaaag aaaattttgg acagatcgat
     4381 gaattactac cgtttggctt tgctggaatg gtagtctcat ttcctatggt tttattcagt
     4441 tttgctggaa ttgaaatgat cggcttaacc gttggcgaga caagtgatcc gcaaaagaat
     4501 ttgaaaaaag caatcaactg tctgccttgg cgtattctct ttttctatat aggaacgatt
     4561 ttgtcgattt tatttgttgt tccatggcat tgtttagatt taaaagaaag tcctttagtg
     4621 actatgctgc aagtgatcca ttgccaaggc ggagcagcaa ttgtcaatct aatgattttg
     4681 gttgcttctt tgtcctcagc aaatagtgca gtctacagta ctagcaggat tttatatcaa
     4741 acagcaaaag aaaaaagttt gccccattgg ttttgtgcgc tttctaagaa aggcgtacct
     4801 ttacacggta ttttaattac aggaatgtta ttttttgccg gtctttgtct acattttttt
     4861 gtagctgata gtcgattgtt ttttcagctt ttggcaggca tgacaacgag ttgttttcta
     4921 tttgtgtggt cttcgatctt gtgtgcacat gttcaattta ttaaagaaaa gaaacggaac
     4981 actacacaaa gatataaaga tcttgctttg ttggtgtttt ttacttcaat tgctttcagc
     5041 ttgttattcg aaagcacgac acgctttatt ccttttttat tggttatctg gtttgtattc
     5101 ttaaattttg tatataaaag aataagcaat agaaatatca gttaggccca gatacatttt
     5161 gcaaaacgaa ttatttcaga cccccctgaa ataatcgata cgcctgaaaa aacttccctg
     5221 cgtataataa cagtatcaat taaacaggag gggataggat gaactggtac agattgacgg
     5281 tagaagatgt attgaaaaac tatcaaacat caacagaagg cttgtcgcaa gaagaacgac
     5341 agaaacgcct gactaatcaa ggatataatg aaataaaagc aaggcagcag acaaaacgat
     5401 ggaaaaaaat agcaaagcat tttactgatc tgctgatgat cgtgttgatc attgcctctt
     5461 ttttgaaatt tgcttctagt gaatatattg aaggaagtat tattttattc gtagtgatcg
     5521 tcaatggact cgttagttac tggcaagaaa gaaaagcgga agaatcattg aatggtttga
     5581 agcagctgat gggacaagaa gcgatagtac tatcaaacgg gatgcaagaa aaaatccctg
     5641 cgaaagaact ggtaaaagga gatgtcattg tcttacgtgc cggagatgtc gttccagcag
     5701 atatccgatt aatcgaagca catgatttat tagtggaaga atcgattttg acgggagaat
     5761 cagaagcatc tgaaaaaagt catgccgttt tgacagaaga agaatcgatc ggcgaccaga
     5821 aaaatatggg tttttcagga acgcttgtac aatctggttc tgcagtcggt gtcgttgtag
     5881 aaacaggaaa cacgacagaa atcggtaaaa tcaatcaagc attgcaatcg gtcgaacaac
     5941 aaacgacgcc tctgattaaa aaaatcaacc gattaaacaa acaaattttt caaggcattc
     6001 tttgtttgag cctattctta gttattttta cgacatttcg atatggtatg gattggcata
     6061 tccttctttc ggctacgatt gctttggtgg tgtcaatggt acccgaagga ttgccagctg
     6121 ttttgaccat gattctatcc gttggagtac atgaaatggc aaaagaaaag gcgatcatca
     6181 aaggacttcc ttcggtagaa acattaggtt ctatgacagt gatctgttca gataagacag
     6241 gaacattgac aaaaaatgaa atgacagttg tggatgtagc tgcaaaagaa gaagcctgcg
     6301 tgttgtccat tatgaaaaac tgtcaggaat taaaaacaaa agaagaacag aaaacagaaa
     6361 atttaagtgg caatccgaca gaagttgcgc tgattcgaca cacagaaaca gccaatctac
     6421 ctttgaaaaa aatagaagca aagattccgt tcagttctga atacaaatat atggcaacga
     6481 tgcatgcgga ggaaaaagga gcggtgattt acgtcaaagg tgcaccagaa gtattatttg
     6541 ctaaatcaac tctttcttct gcagaacaag aagcatggag tcagacagct gcagaatttg
     6601 cgagaaaagg tcagcgtgtt ttaggatttg cttataaaaa agtagattcg aaacaggaac
     6661 tcacgcatga gactttaacc caattaactt ttgcagggat cgccggattg atcgatccgc
     6721 caaaagaaag tgcagtcaaa gctgtaaagg aatgccaaca agcagggatc tcagtgaaaa
     6781 tgattacagg ggatcacaaa gacacagcaa aagcgattgc ggaacaaatt ggcttaaaac
     6841 atactgccaa agttttagaa gggatcgatt tggatcttat gtctgatgaa gagttgattc
     6901 agcaagtgcc aatagtcgat gtctttgctc gaacgacacc agaacataaa ctgcgaattg
     6961 ttaaagcttt gcagaaaaat ggagaaatcg tcggaatgac cggagacggc gtaaatgatg
     7021 cacctgcttt gaaaagatcc gatgtaggaa ttgctatggg aatcaaaggt agtgaagtaa
     7081 gcaaacaggc agctgatatg gttttaggag atgataattt ccatacgatt gcaaaagccg
     7141 taaaagaggg ccggcggatt ttggataatt tgcaaaaaac catcaatttc ttcttgccaa
     7201 ctgcattagc tcaaggattg atcttgattt gggcacttat gctgaatcgc ccgctacctt
     7261 tgtccccagt acagatttta tgggtcaata tggtaacgac aatcacttta tcttatgcac
     7321 tagggttcga gtcgccagat ccagaaatca tgaagcgccc accaagagat ccaaagcaag
     7381 gaatcctatc tggttatcat ttgtttcgga tcatctatgt ctccctactg atcatggttc
     7441 ctgcctattt attggctatg cagtttgaag ggcaaagctt acagcaaacc attcttctgc
     7501 aaaatatcgt cttagctcaa gctgtttaca tgatcaattg cagagagttg tccaagccgt
     7561 cattgaacca aggtcttttg caaaacaaag cgctttttct ttcattaggt atccttgctt
     7621 tattgcaagg gatggttatt tttctaccag tcggccagca gctgatcggt acagtaagtt
     7681 taacgatgac acaacagtta ttgattggag caaatgtgat tcttctattc ttagttgtag
     7741 aaatagaaaa agggatcatg aacaaactgt ttcgcaaaaa agaaaaaaca gctagagaat
     7801 acagtcgcta gccgttacta taaaaaagtt ttgaaagtga gttcagtcaa acgattgagc
     7861 tcatttttcg ttggaataga agaaatgtat acaataggga tatctagaga atctagaggg
     7921 aaattggaaa tcaagatatc tgttttttca aaagaaatag tggaaagatc ggttagttcg
     7981 ataggtaaga agcagacacg ttttcctaag taatctttta attcctgctg caagaacgcc
     8041 ttccatgaag gttctccttg gaaaataaaa tagacattga acaaattcgg tttgatagaa
     8101 aaaatcgttt gttggatcag tagagacaag agttggagaa agtaatttgg ttcattgatt
     8161 tctatttctt tttttagatc gttcagtaaa ttctctttct gttgatacaa gtgtgcatat
     8221 tgctgatcat atgtttctaa aaaacgatgg tattgacggt ccaatgcttc aatcaatatc
     8281 ggcgattcat gatattttaa tatgaggaga gccaagttat cagtcagaga tgtttttttt
     8341 agttgatcaa gagacagctc tgaagctagt tcctctacaa acgtttcgag gacctctaaa
     8401 aatggagaat gatgcaggca gcgagcggtt tcttgtccat agttattgtt gtaattccaa
     8461 ctttccagaa agcagaaaga agcaaaagaa agctcagtgt ctttaagata aataccttcc
     8521 gttaaataca attttttcaa tgcagagtca tacagggagg agagtgtgct gttggtatgg
     8581 atagaagaat ctaatcggca attcgctttt atacggatcg tattgataaa gaaccaacat
     8641 gttccgaaaa tctcttctgt ttcaaccgct agttcagttt gactgaggtt tcttaaaaat
     8701 tgaaaataat gggattcatg gatcgaataa tcctcgaaaa aatagttgtt atgagtgaaa
     8761 ggaatcagca atcgatgata aaaagtacga atcgcacgct cttttccaag aatacaagct
     8821 gtatatttcg tcaaatcgat ctgcaagtcg taacttgcta aacgctgatt gattttagtc
     8881 acatgtcttt tcaatgtccc ataagaaagc ccatggtcgg ctagaaaatc agggacagca
     8941 actttttttg taaaaagcag atttttcagc agttcgacac tgactgattg tttgaaaaaa
     9001 atctcccaaa ggtcatttgt taataaattt tgctggtttt gcaaactgat tcctgcttga
     9061 tcgctgatga tgtcccagcc ttcgggcagc tgagagcgaa tagattgaag gtcagaaaaa
     9121 atcgttcgtt cggttgtccc tatgatttca gctaattcat ttacggttat ctttgtatga
     9181 ttcagcaatt gttctactaa agaaacatga cgtcgaatat ctttttcggt aatgattttt
     9241 ttcattagtg aatacatcaa tcagactccc ttctattcta agaatacgga gaaaataaac
     9301 ggtggtcaag tttcaactga tttttcaatc attgtttatt tttctggagc atttactgat
     9361 gataagaatt tcatcatgaa cttgaaaaat gataccacta aaatcaaaaa ataggtgggc
     9421 aaacaataat aaacatttta atagtagatg gtagcaagac caacacaggc ggattgaaca
     9481 tttaagagtt gataaaaaag aaaaatatcg aagtatttga ttagcaacta cttgtattca
     9541 tttttctatt cttacgtggt atctttgaat aaagcgaatc aataagaaag agatggaagc
     9601 agatgctaac gaaaaaagaa ctaatcgatc acatcaatga aacatacaat ttgtctccag
     9661 aatatatttt taaaaaattt cctgattatt gcgtattcag acatcaaaaa agcaaaaaat
     9721 ggtttggggt cctgatgtct gttccagaag aaaaaatcta tgatgatgca agtgaccata
     9781 ggatcatcaa tattcttgat ttgaaggtcg atcctgaact tggcggaatt ttgcgaggaa
     9841 aagaggggta ttttccagcc tatcacatga ataaagaaca ttggatctct attgatctac
     9901 gtaaaataga aaagctagat gaactagcag atttgattga tggaagtttt aaatcaacgc
     9961 aatgaatgtc taggacaata gagaagaggt tgtgacagaa atgtttagct tcaagaaata
    10021 agaaggaatt tacgaaaatt gcttctcaaa tttttgggaa atttcggctt attttctaag
    10081 aaattacttc tggaacaccg tttgttcgaa aaaagagctg cgacaagaat tttgtcccag
    10141 ctcttttttt tttattttcc taaataaaat tcccgcatcg tttttgcaat cattttgatc
    10201 aaaggatgat cttctgtgaa gctagaagat aaataaattg ttcgtgtcaa gggcaaatct
    10261 ttgatttccc gcaagacgaa aggatcattt ttatcgtaag accaagaaac ttttgggaaa
    10321 aagctaacag ctaatccttg cttgacaata gaacgtaatg tcgcaggatc atctgtcgtc
    10381 gaactgatcg tcaattcgat acttttcgct tcaagtgctg catccagtgt atgacggaag
    10441 gcgttgctct ttgacagcat caagatgggg gtgcgttgga tatcttctaa ttgcagatgt
    10501 tcttttttag caagaggatg tttcttagga atggcgacat aaacaggttc tttcatgata
    10561 ggaatattgg tcatgccagg cttcatctcc gaagtgatgt acagtacagg atcggtaata
    10621 tcatctggta aatgatgttg catgatcgtt aattggatat caggatgctt ttccataaaa
    10681 attcggacca agttcggaat agaaacagag gctacctcta aatacaatat gacggaagta
    10741 acgatcttct cattgaccga agacaataat ttgattcctt cttctaatgt gtctaatgtc
    10801 ttgactgcat atggataaaa ttcttctgca taaacggtcg tctcaatgtt tctgccgcta
    10861 tggtaaaaca aagggacacc taattcgttt tccaaatgtt tgatcgcacg gctcaaggca
    10921 ggttgagaaa taaataattc tttggctgct tgggtaacat tttttgtttc gaatactttt
    10981 ttaaaatact gcagatgaag caatttcata attatccatc ccatccatac ctttttgtta
    11041 tggttttttc tttttttggc attttttatt atagcagaaa ttgactaaga tagcagtgga
    11101 aaaagtaagt gctgaggagg cgaggctcat gagtacaggt ttcttatttc ctggacaagg
    11161 cagtcagaag ttagggatgt tagcggatat ccctgaaatg tacatggaac gtttagaaaa
    11221 tgtgacgggg tatcggttag ttgataaaga agaaaactat caagatacgg tttttatcca
    11281 gctagcatta ctgacaaaag cagtatttta tttagatgaa atggaaaaaa acggtattca
    11341 gcctgatatt gcagcaggac attcgattgg tgctttttgt gcagctgttg cttgtcgatc
    11401 tttgagattt gagaatgcag cggaactagt ctatcaccgc gcagtcttaa tgaaagaagc
    11461 ttatccaagc ggttatgcaa tgggcgtagt agtaggtgtg acgagaagcg aagctgagga
    11521 aattgtagca aaaacatttg atcctgacca tccagtttat ctgtcaaatg aaaattgtcc
    11581 gatgcaacat acgttatctg ggagtatcga aggtttatcc gttacgttgg cagaagccaa
    11641 acggcaccaa gcgcaaacag cgaagctgct gaaagtccct gtgccatctc actgtctctt
    11701 gatgaatgat acggtcaaga aattcaagcc gtatatgcag tctgtccatc tatctgatgc
    11761 tagatgtttg tatataaaaa atacggatgg aagagccact caggacgcag aagagattcg
    11821 taaagatttg ttggaaaacc tagctttccc tgtgcagtgg aaccaaatga tggacttggc
    11881 aaaagaatta ggaatggatc tgacgattga atttcccccc ggcaatactt tgacaaagct
    11941 gattcataca aaatttggtg tagataaagg gatccgtacg atcaatctag accaacatgg
    12001 tattgatgac gccacgtttc tatatcaaaa atggagatga ctaattatgg aaaaaccaaa
    12061 ccgcaactgg gcttctaaac gcgaaaaaaa agcgcagcga ttggaaaaaa tcagcaactt
    12121 gctcaatggc aagtatgtag aaactgaaaa aatcgtagaa gtaatggaaa aactgattgc
    12181 tcccggcgac aaagttgtat tagaaggcga caatcaaaaa caagcaagtt ttctttcaga
    12241 aagtcttgaa aaagtagatg cagaaaaagt gaatgatctg catttgatca tgtctagtat
    12301 ctcacgacca gaacatttga atatttttga aaaaggaatc gcaagcaaga tcgatttctc
    12361 ttatgctggt gcacaaagct tgcgtgtcgc ccaaatgatt gaagacggca caatgaaatt
    12421 aggtgatatc catacttatt tggaacttta tggacgctta ttcatcgatt tgattccaaa
    12481 tgttgtttta gtagctgcag ataaagcaga taaagacggc aacttgtaca caggtttcaa
    12541 tacggaagaa acaccaacaa tcatcgaagc tgcagctttc aaagatggta tcgtgatcgt
    12601 tcaagttaac gaagttgtcg attctttacc acgtgtagat atccctggtg actgggtaga
    12661 cgttgtcgtg aaagcagata aaccttatca attagaagct ttattcacac gagatcctca
    12721 aaacatcaca gaattgcaaa ttctaatggc aatgatggca atcaaaggaa tctacgcaga
    12781 acacggcgta caatctttga atcacggtat tggattcaat acagcagcta tcgaattgtt
    12841 actgccaact tatggtgaat cactagggtt aaaagggaaa atcgcgaaga actgggtatt
    12901 gaacccacat ccaacgatga ttccagccat cgaatcaggt tgggttgaaa gcatccatag
    12961 ttttggtggc gaagttggta tggaaaaata tattgctgct cgcccagatg tcttttttgt
    13021 cggtaaagac ggcacgatgc gcagtaaccg aacattatca caagctgcag gacaatatgc
    13081 catcgatatg tttatcggtt caaccttgca attagattac gaaggaaact cttctaccgt
    13141 aacaaaagga cgtttatctg gttttggcgg tgcgcctaac atgggacata atcctggcgg
    13201 tcgtcgtcat tctacaccag catggcaatc acttcgcgac aatgatgatc cattagggaa
    13261 aggccaaaaa ttagttgttc aaatggttga aacatatggt tcaaataaaa aaccagtctt
    13321 tgtcgaagaa ttagatgcac tagctgttca aaaacaagca ggcttagcca atgcaccagt
    13381 gatgatttat agcgaagata ctacacatat cgtgacagaa gaaggaattg cttatttgta
    13441 caaagcaaaa acaaaagaag aacgacaagc agcaattgca gcaattggcg gtgttacacc
    13501 tcttggaatg actagcacga aagaatcact agatagcttg cgtgcagccg gcatcgtgaa
    13561 attaccagaa gatctaggca tcaaacgtag tgaagcgaaa cgttcattat tagctgcaca
    13621 aaccatcgat gatttgatcg aatggtcaga tggcctgtat gaaccaccaa tgaaattcag
    13681 aacttggtca taaaattggc gagaaaaacc ttttgtcgcc atttacccag atggaagaag
    13741 aaagacgaat aagcagaacg tgtatgctct gttttacaaa aaggggagag atccatggaa
    13801 caattagctt ataaatatcc aacgaaacag ccgatcaata agaaaatcca tgtaggagtg
    13861 gtcggttcag gagatttgga aatcttgatg tttccaacag aaaaaacaga atctactgta
    13921 aaaatttgta caggaagcga tggcttcggc gaagtatgga acaatgtatt aacgagattt
    13981 tttgatcgtt atccaatttc agcagatatc gtgatcaatg acttcggtgc tacaccagga
    14041 gttgtgtacc tacgattgac gcaagcaatg gaggaacttt cagatgaaaa atagttttgt
    14101 agaattaaac gccagagaac gagtgaacgc tctacttgac aatggacgtg aattgattga
    14161 tccattcgat caaatcatcg cgccaagttt accagcacaa ggaatcgtcc ctgaaagtga
    14221 tgacgggatc gttgtcagcc gtggcacgat caatggcaaa caagcagtag taatcgctat
    14281 ggaaggaaaa ttccaaggtg gcggtatcgg tgaagtttcc ggtgcaaaaa tcgttgctgc
    14341 acttgaacat gtgttagaag aaaataaaaa aggaaatgag atctttccag tcattgtact
    14401 agatacaggt ggtgttcgtt tgcaagaagc aaactacgga ctgttatcta tttcagaaat
    14461 cggaaatgta atcattgctc taaaacaata tgtaccagtc atcggtttga ttccaggacg
    14521 tgtaggatca tttggcggta tgtcgattac ttcagcatta atgagttatt taatcgcaac
    14581 aaaaaaagcg agagtcggtt tgaatggacc agaagttatt gaacaagaag caggcgttcg
    14641 agaatttgat tctagtgaca aagacttgat ttggaataca ttaggatctc gtcaacgggt
    14701 agaatcaaaa atcattgatg aattagtgac tgatagtgta gaagcaatca aagctgctgt
    14761 catcgcagca atggacgaac gaaaagacac gcatcgcagt gaaagctcag cattttattt
    14821 atcattactt gattctttag atctttcaaa accaatggcc attgacgatt acaatgaagc
    14881 aattgctaac catcaagcaa aatcaattga cttaccagaa gtagcagaag gaaatgaacc
    14941 agctgcagca agtgttggtt acgattggtt tgcggcacta actggcatga aaaatccaac
    15001 ttcttctatt agtactgtgt attatggtaa atcaagtaaa ttcatagaag aagccatcgt
    15061 gacatcaatc gtacctgatc ctaaaaaccc gatgtaccgt gtacgaaacg gtgaagttgg
    15121 tttagttgaa ggtttccgca tgggacaaat tttggatcac gtttacgaag aagataaaga
    15181 caaaaaagtg aaacggccaa tcatcgttgt gatcgatgta ccaagccaag catacggcta
    15241 taacgaagaa ttgatcggta tccatgtggc tttggctaat agtgctgctg cttatgcaaa
    15301 actccgtcaa gctggacatc cagttattgg attgattgca ggaaatgcta tttcaggtgc
    15361 atttttagca catggattac aatcaagccg cttgattgct ttaaacaata aagaaattac
    15421 cgtccaagcg atgtctaaag aaagtgctgc acgtgtgaca aaacgttcgg ttgctgaaat
    15481 cgaagcagct gctgaaaaag ttccagctat cgcttacgat atcaataatt atacaaaact
    15541 aggagcgatc tatcatttcc tagaagatat caccgaccaa gctgcttcag aacaaaatgt
    15601 cagcaaagtc atcgaagcaa tcaatgaagc cattcaagat gtccgcaata caaatgacac
    15661 gatgctgaca aatcgataca caaacgaaac agccaaaaca ttcggacgtg tcgaaacaaa
    15721 taaagtccgt gccaaaatgg acgaagaatg ggatgcataa aaaatgattc aagcacatga
    15781 tattgtcctt tttgataaac atgaaataga agggttcacc ttgcctgaat gggtcgttct
    15841 aactgaaaag atgttcggaa cagttcgtcg tgcgtcgacg gatcacgcag aaatactacc
    15901 gattggttta cgtggacctg cccgcaatca gagatggtca acagaaatct caaaacatgc
    15961 cgtcaaacaa gtcattcatc cgtgggacat catcgaacaa gaatcgtata gacaagagaa
    16021 aatcatggat tatcctgttt atcagcaatt tcatgctgct agaaacatat tatcaggatg
    16081 taaatgggga atcggcggca gcttaggatt tgaattatcg actgggattc cggcagtaaa
    16141 agaaaccagt gattttgatt tgctgctgta cgcaaattca ccgattgaat tgccaattca
    16201 ggtaattcaa tcgcatcctg ctttttttga gcaattcgat acccaagtag tgactcggca
    16261 aggcggtttt tcactaaaag agtatttgag agagccagag aaaaaaattc tattgaaaac
    16321 agtcgaagga cctaaattaa ccaaagaact ttggtaataa agaaaaagag agagaaagag
    16381 atgtttgaaa acaaaaaaat caaaatcaac gtaagtgact tccagaaagt atcagctgta
    16441 acggctgtta tgatgcaaac gatattgagc tttgctttgg caaacagcca cagcgcaaca
    16501 aatacagctt ttcttggtac cttttacgtc atcgttaaat acacagcacc aatgtttatt
    16561 tttgcaattg tttataatat ggtaaaaaca agtcagcatt tgtcttattg ggaatttttg
    16621 aaagaaaagt tttttgaact agtcgttccg tatcttctat ggacgacggc ttatctttgg
    16681 ctatttccag cagtccaaca acaaactccg tatacggata ctgcaagctt tttactgaaa
    16741 tatgtcacag gtgatggagc gcctcattta tggtataccg ttatgatgct gcagatccaa
    16801 ttgttcatgc cgttttttgt ttggctcggt tacaaagtat ttgctaataa aaaatatgta
    16861 tggccagtat tgattggttc aacgatttta tatgtcgcat ggtatctgtt ctatcaaaca
    16921 caagtattga ctgggcctta tcatgacagc tggtacttat tagatcggtt tgttgctagt
    16981 ttcatgattt atggggtagc cgcatttgtt tatcacgaaa aagtctatca atatttggac
    17041 agagtacgtt atcttttctt acctgttgcc ttagttatcg cgttcttttc tgtccgttca
    17101 ttgctcgcac atccaggaga cttgagcttt gcgaatgcac cttacttgaa tacgatccaa
    17161 agtttataca gtttagtcat catctttgct gtcttcatag gcgcttccaa aatgattgta
    17221 aacgattcgc caaaattgcc attgttcaaa tggttgtctg tttatgctta tcgtacgtat
    17281 ttagccaatg tatttgtttt ccaagtactg ttattattgt ttaaagatag ttggctgcag
    17341 ttaccttcag gagtcatgat tcttgtagct tatctaatga cggcaagttg tgcatttgct
    17401 ttaagctggt tacttcatat catttgggta gctatcaaaa aaggtttcag caagtaatag
    17461 taggaagaag gagtgagtgt catgataaat gacgaacgaa aaaaagcagt gactcagcaa
    17521 ctcgctgcgt tggtagagga agcattgatc gcagaagtaa cattaagtcc aaaacctgga
    17581 ttagtggatg cactggatgc gggtgcacat tccgatatgg actatgcttt atttcttaag
    17641 tctgctaaga cgctaactcc tttttttgaa gaaatggctc aaatagcatg gcatcatgct
    17701 attgatcagg agttaagaga aagaattgct gagattgggc gaaaagcgga aaaggcaatg
    17761 ctgattgcta caaatggcat caatacccat aaaggagcga tttgggcaat ggggctattg
    17821 atcagtgtca ttgcacaaaa aatcagtatg gaacagcctt tttctttatc gaaaatactt
    17881 gatgatgtca gcaagcttgc tgcttttcct gatacaaaat atcgtcataa aaaaaccact
    17941 catggtcata gggtgaagca aaaatatggc gtaaatggtg catatgaaga agcgatatta
    18001 gggtatccgc atatcagact tgcaattcaa gcagctaagg agctagataa tgattcatat
    18061 gtacagaaac aaatgcatat gttgttagtt ttgatgagtt cgctagatga cacatgcgta
    18121 ttgcatcgca gtgatcagaa aacgttagtg gaaatgcaaa aacttgctca tgctgctagt
    18181 caaagtgctt taccaaatcg tccatttaca gcactgttgc acttttgtca ggaaaaccaa
    18241 atctctcctg gaggaagcgc agatctcttg tctgcaagct tgttcatgct gaagatagag
    18301 gaattatgga ctgccgacac atacatttgt ccgatgttac tcaatgacta aataagacaa
    18361 aattgctaaa aaaacagttt atgcatctgg ttattgactc ttccttttag caaggctatc
    18421 ataaaaatag ttttttgtaa taaaagataa cgaggaagac aatgaaaaaa atcaattatt
    18481 tagcaatcat tttgttagtg ggcctatgtt tgtttgctct gccgacccaa ggattcgctg
    18541 tcgcgggtgg tcatggtgga ggtacatcta gcggtcgaag ttcgggcaga ggttcatttg
    18601 gtggtcgttc atatgggggc agttattttg gcagtcgttc ttacggcaga gattcttatg
    18661 ggtattatgg tccaggtaca ggtccttcta gttatgagca aacgggattt cttgcattag
    18721 ctgttttgtc agtaggaggg gtttctgctt tttctatcgt caagaataaa agacaaaaga
    18781 aaaggctgaa agcggagata ttgtcccgca tgtcaggaac ggctaaagaa aagaaagcac
    18841 agtttctgga aatccaaaag atattcatga agatccaatc cgcttgggaa gaagaaactc
    18901 ctgaaaaagc cagaagctgt tatacagaca agctgtttgc cgaacatcag aaagttctgc
    18961 aaaaaaacaa agcagacggt gtaagaaatc atactaaaaa aataactatt caggaagtag
    19021 gaaattatcg acagatccac gaaaatagtt ttgccatccg aattgatttc agatgctgtg
    19081 attattcagt cgatcgaaaa gatgggcgat tgatctcagg aagtaagaca caacgacagt
    19141 atttttctca gatatggtat tttgattttt ctgagaaaga aaaaagatgg caagccgatt
    19201 ttattcagcc gattatgctg gattgacagg aaatatttat gaacggagtt gtgacaaaag
    19261 ccttgtcgca gctccttttt ctgaataaac ggtgtgccag agatagccta tcggcaataa
    19321 ggcacgattt tgtaaaatat gggaagcgat ttttcaaaat cgcttcttat cgctcaaggc
    19381 tgaacatctc tgtcacaacc tctccactaa atggtgttta aaagcttgca tctgcgataa
    19441 taagaacaac caaacgaaaa ataggaagca ctattttagt ttagttgttc ttattacttg
    19501 atgcaggaca gctttttcac aacctctttt ttgtatagat gggcaaaatt tttgtaataa
    19561 taggcgtatg tctttttcgt tgaggagatg aaaaagcata tggcttattt tttgtttgct
    19621 ttgcttactt tctttccact tcaagtggca gaaatatcta ctttttatta tgtaagcgga
    19681 accatatact tgaaatcaga aggagatcat ttctatgaaa acaagaacaa ttgaaatact
    19741 tcacagaatc atgacagccc aatatccagt aaaagcaaaa catctagcaa gtgagttcaa
    19801 tgtcagtcag cggacgatcc gtcaggaagt tcttgaagcg aatatctggt tgagcagtaa
    19861 aaagcttccg gagatccgta caattcgcaa caaaggtttt ttgttgaagc tggaaaagga
    19921 aaagcaagct cgattagaaa ctgaaataaa agatgtgaaa gacggctttt tgaatcggcg
    19981 cgaacgtatt tttgatctag ttttagccgt tgcttacagc cgaatcccga tccatctaaa
    20041 caaaaaagaa gaagaatttc aaatctctaa aagtacatta gatgaagata tgagacggtt
    20101 acgagcaaca ctggtcagtt atgggataga gatcgtcagt tatggaaagc aaggactggt
    20161 ttataaaggt tcagaacgat ctatccgtac gatgatctat gattttatca atcagaatct
    20221 gggaatgacc gacttcttta tgaatcaaga tgcacaatta acaccggctc aaaaaatctt
    20281 tctccattat tttcctttgg tggaaatcaa aaaaatacag gagatctatt ccaataagtt
    20341 gttgaaacaa gaagatgata tttataaaaa acaaatcctg ctttttacat tgatctggat
    20401 ccagcgaatc aggaaacatg aatttatctc tgccattaat tggaaaaata cagaaataaa
    20461 agacagtagc ttttctgcat ttattgacca catgatcgag gaatttcaat tggacaatgt
    20521 cccgcaagta gaaagaaatt atcttcgttt cactattgag acgttcaatg ctagagatat
    20581 ctccaattca atcgagtggg tacaagctca attactaacg atccaattga tccaatttgt
    20641 agagaacgag acacacgtac ctttccatct aaaggaagaa gctttattcg aaaatctcta
    20701 taagcacatg gctgcactga ttgtaagaat caagaatcat atccaagtca tgaatccatt
    20761 gaaagaaaac atccgaacaa attatttccc aatctaccgc gcagtcactc attttgttcc
    20821 tacgatcgaa gaagtaattg gtggcaagat actggaggac gagatagctt ttcttgtcat
    20881 ccacttttcg acgatcgcca gtgtaatcaa gcgagacttg aattatgttt ataaatcagt
    20941 cgttgtctgt aaccatggga tggctactgg aaatctgtta gcagagaatc tgaaagaaaa
    21001 gtttcctcag atcgagatta ctgcagtttt aagttcaaaa gaaatcgaat tagtagataa
    21061 attggatgtc aatctggttt tcagcacctt tcaattgaat tatcgaaata agccgctttt
    21121 agttgtcgat ccgattttaa ctgagacgaa tatcttggtt atagaagact ttttgaaaag
    21181 aaatgaagaa ctacagcggt tagagcctaa cagtcatgaa tcgacggagt tattcactca
    21241 aataatagat gtcatgaaag aaagtggtgg ccgagtagat cgaatcatct atggcaagtt
    21301 agaaaaatta ttcgaaacaa acaatttaga aatcaacaaa agggagatcc agcctatgtt
    21361 aaaagatatt ttgacggaca gcaatatcat gatcaaagaa aaagccacta cttgggagga
    21421 atcgattgaa ctagctgcta gaccattggt taaagaaaac attgtcgaag agcgctatgt
    21481 cgaagcgatg attcgtgcag tgaatgatta cggaccgtat atcgtgattg gcaaacacat
    21541 ggcactagcc catgcccgac cagaagatgg ggtcaataaa ttaggagtca gcgtagctac
    21601 gattgagcaa ccaatcgatt tcggtaatcc tgaaatggat ccagtaaaga ttattttttg
    21661 tttggcagca gtagactcct actcgcatct gaacatcatg aaagaactaa ttgaattgat
    21721 caacaacgaa gagaagttgg atcgattgat tgaatgcaca aatatcgaag atttcaaaca
    21781 actgcttttt tcagtagttg agaaataata aatgatacgg gggagaacaa agatgaacaa
    21841 attgaacatt ttattcgtat gcggtgcagg tttaggaagt agttttgcag cacagatgtc
    21901 tgctgaggat gttttgaaca aattaggagt agaagccaaa ctggaccatt gtgatatttc
    21961 ttccgcagtt tccatgaaac cagatgtgat catcacagca gaaaattttc gttcgcagtt
    22021 cgaaaagttc gcagttgatc ctaaaacggc gatgatctat ttgaaaaata ttgtttcaaa
    22081 aaatgaaatt gaagaaaaaa tcaccccagt tctgcaagat aagggagtta tctaaataca
    22141 aaaagaaaag agggtaaaaa catgggcgtt atcaatttta tcatcgaaaa tattttgact
    22201 caggcatcga tcaccattgc attgattgct atgttgggtc tactactgca gaagaaatca
    22261 gcaggacaag tcatctctgg cacgttaaag acactcctag gatttcaagt gttgagtgcc
    22321 ggttcgagca tcatcgttgg aagtttaact tatttcggga aaatctttac tgaaggattt
    22381 catatgcaag ggatcatccc ttctattgaa tctatcaatg gtcaagcaat gaatgatttg
    22441 ggtttaggta gagatattgc actgacgttc ttagccattt ttgtattcaa tattattttg
    22501 gctcgtttca caaaatggaa gtacattttt ttaactggtc aagcaatttt atggatggca
    22561 acgatgacga cagtctttgg ctattttgcc ggtctgcgag ggatcgtatt gatcttggtc
    22621 ggtggtttta tcggagcttg tttcgccatt gctatgccgg cagtagcgca accgatcatt
    22681 cgtaaaatca ctggatcaaa tgatattgct ttaggtcatt tttgtacgat cgggtactta
    22741 tttgaagccg gtgttgctaa gctttttggt gagaaaggcg aaaataaaaa gtcgatcgaa
    22801 gatatcaagt tgccgacaca tttcgaattt ctgcaagata cttatttgtc tgtcatggtc
    22861 gtgatggtcc cgctatatat catcactgtt ttatttgcag gcgaaccatt cgcttctgaa
    22921 ttatcaggag atcaaaacta tatcatgttt gcctttttac aagccatcca attcgtagtg
    22981 ggtgtgtatg tattgctagc tggtgtacgt ctactgttag gtgaaatcgt gccggcattt
    23041 cgagggatag ctatgaaatt agttcctgat gcgatccctg ccttagattg tccagttttc
    23101 tttccttata gcccaaatgc tgtcatttta ggtttcatta cgacaacgat tggtacgatt
    23161 attgccatgt ttactttgcc catgttcggc ttagcgatga ttttacctgg tatgctaacc
    23221 aatttctttg ccggagggac agcagggata tttggcaacg cagttggcgg acgccgagga
    23281 gcgatcattg gcggtattgc tcatggtttc tttattacat tattgcccgc tttgctagta
    23341 acgatcttta attcgatggg attcaacaat gctacagcaa ccgatgtaga tacagtagcc
    23401 gcagcactct tatatgcctg gatcttaagc ccagttttga aagcatttta attgaaagta
    23461 gggaaaatga tgtttggatt ttttaagaaa aaagaaaaag aaccgaaaaa agttctggca
    23521 aaagaagaaa aagccctatc gccagaagcg accgaaaaga ttcaatcaga gattacacaa
    23581 ttgaaacaag agatttccac tacccaagat aagcataaat tagcgaaact ttatgaacaa
    23641 gttggactta aattttctga attgtatgtg aacgatcaag ccatccaata tttagaaaag
    23701 agtttggaaa ataaacagac gatcggagat gggtataaaa aactgatgag cctctataac
    23761 cagaagcgtg cagatgctgc tcgtgctggt gatgatcaag ggatcgatta ctacatgggc
    23821 aaaatggatg agatgcgaca aattgcaaaa caagtaacga tcaaaggaaa caaataaaaa
    23881 gataaggtgg caataaaaga tgtatacaac attgaaagaa gtaacaaaaa cagcagaaga
    23941 gttgaatttt acagttggag cttttaatac gcataaccta gaaatgcttc ctgctatgct
    24001 aggcgcagca aaagaaatgg gttcgccgat cattattcaa acaagtgtcg ataccgctaa
    24061 atatatcggc atgcatgtgt tagtcaatgt aatgacagct attgcagaag aagaaatggt
    24121 tgatgcagtc ttgcacttag accacgcccg agactttgat gatatcaaag aagcaatcga
    24181 cagcggatat acttctgtca tgtatgatgg gtcccatctg cctttcaaag aaaatatcct
    24241 gaagacaaaa gcagtagtag aatatgccca tgcgcacggt gtttccgtag aaggagaact
    24301 tggaacaatc ggcggtacag aagaaggaat ccatgtcgct gaaaatgaca aggtatatac
    24361 aagaccggaa gacgcagtga aatttgtaga agctacaggc gtggatgcat tagctattgc
    24421 tatcggtacg aatcatggac aattcaaatc aaaaacagaa gtaaacattc ccttgctgaa
    24481 agaaatcgat gcagtagttg atgtccccct agttatccat ggaggaacag gtgtgaaaga
    24541 agaagattat cctgaattga tcaataacgg gatccgcaaa ttcaatgttg gaacggagct
    24601 tttagtcaac tggacgaaag tagctaaaga aacctttagt gaaacagaaa tcaacaagtc
    24661 tcttcgccat aatgtgattc cagcaaatga agcagtaaaa gaaattgtca agcataagat
    24721 tggactgttt atgaacttgg aaagtccaat taacttagga aataaatgag atgaaaaagt
    24781 atttcattct tctggctggt agtcctggta caggaaaaac atatcttatg aataaactac
    24841 gggaacgatt tccagatatg tatgccatca cgctcgatga aatcaaggaa tactatgcag
    24901 aatccatcgg gtttaacaat ttggaagaac gggcggaaca agaaaggacg aaagtttatc
    24961 ctttctatta caaagcactt gatttgtata tggaagcagg aaagaaagtg attgtgtccg
    25021 agtatccttt tagtgataag caaaaagcac gactccaaga attagcagat agatacggat
    25081 atcaggtgat cacgattcgc ctaacagcag actttgaagt gttatggcaa cgtcggtaca
    25141 aacgtgatcg tgaatcagat cgtcatttga gctatatcat ggatcattat cactatggtg
    25201 acgtattgga agatcgaacg aaagggacca atcatattac gaaagaagca ttcaaaaaga
    25261 tcatcgccga ccgaaaatat gacaaattcc agttaggaac actatatgaa tttgatgtaa
    25321 cagactatac tcaagtggat tacactccac tattggataa attagaaaaa gaaatcaatg
    25381 acaataagta acaaagaaat ggcttaatat cgaaaaattg gcttttgaac gctgtttacc
    25441 gagaggtcgt gagcctgtcg ttcagctccg agtcacaagg agcaatttca aaaaaacagc
    25501 ttctcatgtt tgatgaatat tgtgacttgt gaccgaggag ttggtaggag aacaccgttt
    25561 agtggggagg ttgtgaaaaa gctgtcctgc ctcaattaat aagaacagcc agacaagaat
    25621 agctcgtcat atttttcgtt tggtggggct tattaccgca gatgcaagct tttgaacgcc
    25681 atttattcac agccccagag aagtcgaatg tcttttgttc tcgagttttt taacattctt
    25741 ttatttattg cagatttcat tatatttatt tgccgtaatt aaaccagtct tctatacttg
    25801 attacatagt catacatttg ctaaaaaaga agggaagaga atgaacaaga agttggctca
    25861 atacaaaaga ctactcgcac tttctcaagc tggtctatat tatggaaaag atgtatttga
    25921 taaagagcgt tttgaggaga tccggacgat ttcattggaa ctgatcagtg aaataggaaa
    25981 agaaactttc gaagaaataa aagcgctcac aacgattgga gaaggacatc caacaccaaa
    26041 agtagatgtt cgtgcctata tcaaaaaaga tggcaaggtc ctactaatcg aagataaacg
    26101 gacgaaagaa tggtcacttc ctggtggatt tgctgaaata ggattgtctc ctgaagaaaa
    26161 tgtgcgtaaa gaagtctatg aagaaacagg attaacagta gaaactactc agttgcgagc
    26221 agtttttgat acgaataaac aaaaagatat tccacagctt tttcagtatt ataaacttgt
    26281 ttttgcttgt gcgatccacg gtgaagaggc caagtttatt gagaataatg aaacttccaa
    26341 catgggattt tttagtatag aagagttgcc aaagctatca gaaaagcgaa caacgaagaa
    26401 acagttactt attttagaaa acaaaaacca gatctattgt gattgagctt tttcgtaaaa
    26461 gaagaaaaga atatacttta ctagttatta aaa
//
LOCUS       DAZKLD010000022        25761 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.022, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000022 DAZKLD010000000
VERSION     DAZKLD010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25761)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25761)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25761
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.022"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            346..474
                     /locus_tag="ACG8KR_000787"
                     /pseudo
     CDS             346..474
                     /locus_tag="ACG8KR_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 family protein"
     gene            588..827
                     /locus_tag="ACG8KR_000788"
     CDS             588..827
                     /locus_tag="ACG8KR_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0200184.1"
                     /translation="MARPVLEAMDMDTLTKGRYVQLLARGYSPKDVFKSLSKGTDMEK
                     ERLRKEFDYWRTHNGIKDLKPARPVIKRKKKITQD"
     gene            939..1133
                     /locus_tag="ACG8KR_000789"
     CDS             939..1133
                     /locus_tag="ACG8KR_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0200185.1"
                     /translation="MEEKLLKTMKQKHLKRLSVMQYINDMQITGKEKACLLGSMKNFE
                     QLRRTYVKTSSNCQLLLEVS"
     gene            1219..1890
                     /locus_tag="ACG8KR_000790"
     CDS             1219..1890
                     /locus_tag="ACG8KR_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HFQ0200186.1"
                     /translation="MRKWLFGFLGVALIVFCSVFGYVSYQKHEGEVFKQNIEKKMPVD
                     QINAHAKSYKEDATNVNNDMSLGQMLSIQQEAIEMGVNKQVFAQIQIPALGLALPIFK
                     GANQYTLSLGAATYFYEDAEMGKGNYVLAGHNMEMPGVLFSDIQKLSLGEVMDLVSND
                     GVYRYKVTRKFIVPEYFKLIDGVPEENSFLSLPKKGEKPLLTLFTCVYTSQGKERYVV
                     QGELQ"
     gene            1943..3919
                     /locus_tag="ACG8KR_000791"
     CDS             1943..3919
                     /locus_tag="ACG8KR_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFQ0200187.1"
                     /translation="MAINRYVKKAGMLIGMLGIIAGCFQGYRAYAEDVTQKTPPEKVN
                     ITVHKLMYDQGTQLNVDIDGIKNDGYTHDQYPTGVTKYNKADYGDVEFTLGNITDQVL
                     PREDSDLTNAKVDEIVQDVEDKGSNSEYVKNATNKITSAVDENGEITFTDQPAYVNSK
                     GNVYVVYESKSAAGLVTQKAKPMVVIAPMTDKTGSGFLKNIHLYPKNIVSKLSFELTK
                     FGDDGADQSKQTLLKGAKFELYKGEPGKGTKLGDLVSDDQGKLTATDLTLGKYYFVEV
                     PSEVVVGSDQEPTADQYLLGADARNDAHNKLTFEITNDGVTSDLKASYVNYKAPVIDK
                     TVTNGTGQEHSFQIGDAVNYQGTIHIPTDIAGGADGITVNGVKSETSPYSVFKWGDTA
                     GQGLSYVAAKANIKVTNKDGSVVLKENTDYKIQNSENGFVIDFIVNNGQVSDTVASLH
                     GQDLQMTYNMYVNDSAAVANPLTNSVDFVYNNNPFNQEEHHEKTKADVVTYGAKFLKV
                     DSGLFGTGIKATPLEGAEFAAKNAEGKYYGGLVDTDKDGVKEAVWVDDVANAAILKSD
                     KEGHFEITGLTEGEYSLEETKAPENYQKLTKEISFKVDKDSYKEENRITIKNNQKASV
                     PMTGSNGFQTYVLISCLLLGAGALSAVVYFKKKA"
     gene            3934..4686
                     /locus_tag="ACG8KR_000792"
     CDS             3934..4686
                     /locus_tag="ACG8KR_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFQ0200188.1"
                     /translation="MIIRKPKKKRIMGKWIIAFWLLSAVGVLLLMPAEASVAKYQQNQ
                     QIAAIDRTGTAAETDSSLDVAKIELGDPVGILTILSISLKLPIYDGTSDKILENGVGI
                     TEGTGDITGGNGKNPLIAGHSGLYKDNLFDDLPSVKKGEKFYIKVDGEQHAYQIDRIE
                     EVQKDELQRNFVTYLESNPNEDRVTLMTCTPKGINTHRFLVYGKRVTFTKSELKDEEN
                     KKQKLSWKWLLGSTVFLSVMIIGSLFVYKKKK"
     gene            4702..5457
                     /locus_tag="ACG8KR_000793"
     CDS             4702..5457
                     /locus_tag="ACG8KR_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin N-terminal domain-containing protein"
                     /protein_id="HFQ0200189.1"
                     /translation="MRNRKWLFVCAAALFSFTAYTQLTFADRQESTDSTEQTIQTSDS
                     STIQSSDETVDSSTVDSSEEPKIAEDDQIVKQTIHVQKIISSEQLDNQGHEKQEGVNG
                     AKFSVYDVSDILQKMDVKDLTTDQIESQLKNRVKKLSSDQLKLVSNEETKTIDQQTGV
                     FEFSVEVQANQKQAYYIVNESSPENISNSEDILLLTPVSDKNGEFLKDVWIYPKSEAS
                     QPKEEVKKIVSTGVKKNFFENCWDFVTHLFSRD"
     gene            5470..5730
                     /locus_tag="ACG8KR_000794"
     CDS             5470..5730
                     /locus_tag="ACG8KR_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0200190.1"
                     /translation="MKLIKLLLFNFSCLTAVFLYCGLLFSKLSILPSYQLYLMRILNI
                     PAASMPLDIMEKLIRNPLFTTINLIVIVGISLVIYKKEGMKG"
     gene            5727..7817
                     /locus_tag="ACG8KR_000795"
     CDS             5727..7817
                     /locus_tag="ACG8KR_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HFQ0200191.1"
                     /translation="MNFKHSWTRRLKYVFPIMMMLGTLFGLKTANVFAEKVIVDPSNP
                     IQNVKNNFIIPKDVPNAKTNITVNGASKVYKYHVDANKGGFTDDYVGLVEGNKNIRYP
                     SFQKEYGETNLVLEGVTTNTKVNIDYGKIGTYNGKKVNIKLVLSNIHLYSDTLPWNLL
                     DNNYTKTHFRDDGYKNTNGAMSKSKKRTVLWISDNLFSGIVYHSTQMNVQLVATYEDG
                     SPVQFSGDTFISFNSLNPAGGKSTDLKGEYAHYDKMNTTDWYVRKDTVLSEFKSFYNN
                     LNVVGGHPGGSSKLTQADNDFNNLHDKLGDPKFGQGTVSFKISEANPTFVIGSSNVQT
                     WFTLSSATIFSVVPDQPEKTGVDKNGNNVNDKMLQVGDTIQYRIKQKVNRLGVDLLAV
                     YDRFELIDNLPKEVNYVDAHVESGTNKKFDVSGEVTYDKTKHQVKYAAKADTLKNRMK
                     YNGETYELVINVKVNELANQNSVAKNQGTSIINKVEKDTNIITVYFPKIPVKEVQQNG
                     KDVNGRNDGKKGTPTAPLNAGSEVQYLVTQKWHTKGVDAASDHYKQFSIQDPIEARLT
                     YKEGSAQVIDKSKGKDITSEGTLTYDSNSRTLKWEASADFLSNNLLDGREIQLIFTAK
                     TPLQSEKNIDNQAVVAVDNVSNKTNVVTIGVDPNLPQVIVPKTGSTHLVTISAVSLLL
                     LVLATFSYAVLRYI"
     gene            7844..8308
                     /locus_tag="ACG8KR_000796"
     CDS             7844..8308
                     /locus_tag="ACG8KR_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0200192.1"
                     /translation="MKKLIIVVTSLIAMIVILVGCSNEKNKQTNQDNGVLKSGTMWKE
                     EVGGLVYNLKIIDETTWEYSESVWHPDPVQITVKRQKDYKGLERYKIVDSAGVREFIN
                     KSDSLFIVVPYEKNGVKKIIFLGSSKDEKRTKEKLIHDGSQSNKYKLQKTSE"
     gene            8305..8547
                     /locus_tag="ACG8KR_000797"
     CDS             8305..8547
                     /locus_tag="ACG8KR_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5415 family protein"
                     /protein_id="HFQ0200193.1"
                     /translation="MIASLFICPEGGITVAKAKTPFEKAASKILERRGVSETQWKQEV
                     LEKAQFDLLRGKDKDLEGYIYQREREKLVFDEIIKH"
     gene            8565..8867
                     /locus_tag="ACG8KR_000798"
     CDS             8565..8867
                     /locus_tag="ACG8KR_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0200194.1"
                     /translation="MFESITMNFLTQPKVVAAVDIFRALQTAATSLKNNLMIFAAAAA
                     VASIVIGGLFFLFGRRGAENGKMHVGYVLMGVGVILAAASIVVSLFGLFGQTPQGL"
     gene            8937..11000
                     /locus_tag="ACG8KR_000799"
     CDS             8937..11000
                     /locus_tag="ACG8KR_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0200195.1"
                     /translation="MYFTIAGNTKTDKELIKKATKEVGKRTSFLPKTIKYSVIETEEQ
                     LHVLGKVLEHESRSSVKLTLTLHDTEGKIEKAGTEYVFGPLDYQISEGFYPTLMDLIR
                     LDVFKETDETMSVEESYRNEKLLQVLEEELESDQRKVNQGSEVEVNEEVPWERPIFPK
                     KESKEKETVYVDEQADIPTQKSIFEEENDVMGQLEVDPFEETMEDEETAEVEVDPVEA
                     NEESTWTEGDSQEYIEPLISEEVEKNEIEIQKATVFDYILDQYPPEHEWMKEKNKLFL
                     NELYSKYQLPQTYQAFLESKEELISQGKGTLLEQLESVQSTNWHELAQEDLLATFEER
                     QQETDQEINEYATEQSSNWEKAKEELNKEEQRVIEEEVQKIKERYEKKRDNAYSLCVA
                     KINNFTQTNQEALSKEKENLLKERIKERKEEFYRGLRTDKLKISKELNEQLNKLYQTT
                     TEVLMEKYEKIQQELQVQIPKWKKEYEIQQQELKEQEEKQRQKEKEREQLELKKREQD
                     LAEQQMAIEKQRLNQEKEKEAAARKERQEQMDTLRQAQLNYLAQPQPTPSVAAVQTPM
                     NNQTNLTPSPQRSFNGWMVGCIASLSLLLGGGTVFAFNHINQSSQADSVATTTSIKEE
                     AQEQARKEYEQQLEELLNRTNQSTTESSMENSKESDSSKAKEGSSDSTVQSNTSESSE
                     SNEVK"
     gene            11000..13612
                     /locus_tag="ACG8KR_000800"
     CDS             11000..13612
                     /locus_tag="ACG8KR_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirD4-like conjugal transfer protein, CD1115
                     family"
                     /protein_id="HFQ0200196.1"
                     /translation="MRVRETTKRKDKRFNLEKNRYLSHFYRYTPSGEELPEKHDKRIS
                     KKIFYSGLVYLPLEYLVTRVPEVFNRLEIVKGSLQGINLFQLQEMPQVFHFDSAGFWN
                     PFTPTTSLGTRLAISTVCVVPAVLMGLKMDMWFRPLADGQKGDNRLMTLKEIQQIYPQ
                     IPETKTTFPGYGGIPITHYKKYWYIQTDTVNTCIVGTSRSGKGQIEVLATIDNLSRAE
                     KQSSMVVNDPKTELYVGSKDELEARGYDVYAFNITDPLQSMSDNPLALIVKYWKRGDI
                     DTATQLTNTFTNTIYYDANASDNKYFNENAQKAVNALIFALLEQADQTGDYSKLTPNN
                     LVELLSEIGGFNYYPDPDNEFKQLNALDEFMNQLPPGNIAKKQYGATKIGSDKAKGNI
                     LSTAITGLNPFTLTKIAKMTSQSTIDYKSVGFPKYLDLKLHESLLNQRVVVEFYHKGK
                     KFHEERVKVGYKGFCEINFDCHLVAGDQVCLKYDHQGKEYVAWYEFSQRVLKDEEGNI
                     VYRKKRGETHLPELEKEAVLTLDEEKSNLLVESIRMHYTDKPMAIFMLTPDYDPSNHI
                     IVSIFLSQLYKELSTQCVKTKGDKCHRRIHFILDEFGNMPPMDNMEGIMTVTAGRNML
                     WDLFIQSYQQLYAKYGEQNAAIIKENCQTHIYIMSTDDNTIEEFSKKVGNKTVEQENS
                     TINSLGMNTHINRNVDSDRILTPERISTFLEGETIVLAPLKRQDLKGHKIRPYPIFNH
                     GETNLPYAYQYIEDFNPSWDINDIDIHCTHADLDLKALQIDYDYFLPDEYARNLYHGL
                     HDQLEEKNEENMFYKIVQSLENETAKKLLIQFYEEQAADRLQEYVEKLIKNKEISEMI
                     GKRLLLESEKVVKE"
     gene            13609..16140
                     /locus_tag="ACG8KR_000801"
     CDS             13609..16140
                     /locus_tag="ACG8KR_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pLS20_p028 family conjugation system
                     transmembrane protein"
                     /protein_id="HFQ0200197.1"
                     /translation="MIIGGIFSGIADWWSGLFGEGDANPKTVTKVLQDYGNYLQYQDV
                     FSWIWNTFLWGLIKGLYNLNTLLEKTIYQSFDLKDLLNAAGVNALFQSLISKVLALFC
                     VVTLIYLGLKFFMSKHPPKVKNILVNLFMAMILIFGGSSLIDEGLNISKSFYGDVTAA
                     NKADKTGSPAFQIIQNNVIDVSTLLATDPNKVESLPTDKRNALTAKNFKTANINGIIT
                     PKQAKEMADKDKSGLSDLQKDRLRALQYRLELDDNGKEIPVQISDEGLAKYVYTSGYR
                     RYSSKPGIILAGESSLAVAYLFILFTIVTCIIELVFKKFYLVIAASTDFETGQRMKSA
                     IEDISQSFLLLAFTILELRIYTLMLSGIGDLHAAGKINGFLYVVALIVLTIALFKGSQ
                     SVTKIFGVDTSLKNGSNSLLSMFALGNIAKNVGSGAKNLASAGKSGLSHLNDIRKNGF
                     GHRKDVTPDNGEEQSDDKATTVPNSKKTVSEMFGKAKNGVSKAAEGLGYVNERGLKGT
                     AEDLTEKAKDTLEEKLDGTRAKELAEAVKNPSEALEKQKEKASEVKEGLKDAYGEGQV
                     NAAVKANTTTEKENQEGIVIPSVKDHQEALHSNENSIEEKNSIEERNSEEGKNGQLPK
                     TDPEQYRYKPNSDLENEKRISDLSKVNQEDKAENEGQLPKTDPEQYRYRSVNSENEKR
                     ISDLPKRNQEVQTDEDSIVLPNDNGIEVGTEKSTKIGLPKAQNLPMKDSRLNQFKEND
                     QSFVHQSTAGTVQMKPSRLPENGNLTTVSYNLSESGETGNASVQNVVTREAAPKPIQN
                     VHTGYKPANVAFTAPTKINVTRETKNRNNALKEQLLRDIKQDIKP"
     gene            16190..16522
                     /locus_tag="ACG8KR_000802"
     CDS             16190..16522
                     /locus_tag="ACG8KR_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0200198.1"
                     /translation="MDKYINMKNPPRFSGEWKFFAFFFLDLLTLGGCFFWAKMVNNLV
                     HLPLFFALLNYGLWIAIALVIVWRPYNNPGQRQIFAIIESIFFADKNHYHALDPNRNY
                     HGTYKEDE"
     gene            16523..17116
                     /locus_tag="ACG8KR_000803"
     CDS             16523..17116
                     /locus_tag="ACG8KR_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0200199.1"
                     /translation="MRVKGQNSVERKSILQFGKPEERSILDLFPYKSCTEEGVLITRE
                     DHFQRYYRVVSTDVEGLNEQEKLERMNQLTTVMRTYVPTIKLVSLTTETDLTEQIVQK
                     RQLLDKNRMEQSLGRNLQRLKKYEKKLVEEIQELKRAEQERPDLSFFFVIEGKTIKDL
                     QTRNRQLMRSSGILGLKPLYKKELIKILYRMNNMNDE"
     gene            17279..19303
                     /locus_tag="ACG8KR_000804"
     CDS             17279..19303
                     /locus_tag="ACG8KR_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirB4 family type IV secretion system protein"
                     /protein_id="HFQ0200200.1"
                     /translation="MLEAIKTAFSPQNDQKVLDPLKQLGNREIEILKSKGYDLDFLKQ
                     VMPQGGLNFDEEYVTYGNGYSRCVTVYHYTKNPTLFWLANLMNNPHTVATLDVSTDEK
                     QKVIDQINRALDELNDRSDNERKATDRNKSAEEYLELAEYARKLTSNGEISKQVKVRV
                     HVYGNTIQEVDERAQELIENLKGMDYRAVTYLHKTKRDWQSMFTMFDEQAEWFGNIKG
                     QSLPSLNIGGGFPFHHQTLKDPGGTQYGETMTGGPFVWNATYKNSVRLSTNTLLLGMM
                     GAGKSTVLKMIAEAHLAAGDFIWGFEKGKDFIPFLKEYNGIMVRLDGSDGMINPLEIF
                     ATRTYDEASSLYDDGSVKDLKINEAASYQTHLDKVVYQVQLVSPQLKGTMKAEFKTYL
                     NRFYEEYGTVPRGFTSSNSRSNTETQVTGKDPEAYPTFKEFLDFLGQLELPGASQEKK
                     NRKEEMESIVESLCETYGMIFDGHSTIRHLDQQQLVFFDIDSITGLDRGVQQCQMYMA
                     MTLIWNQALIQGRRQRRLIKEKQMEEQDFKRFLAMIDECHNAINPDFIETVNYVTNFQ
                     REMRKVEAMTVLATQSPQEMVPENMSSDNFSKLKKVFEFSSTKIFMRMDESILRHIEG
                     LVGKSMTNSELESIPQQNQGDAVINFGSKETIRVHFSPNKRQLKLFDGGK"
     gene            19303..20502
                     /locus_tag="ACG8KR_000805"
     CDS             19303..20502
                     /locus_tag="ACG8KR_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFQ0200201.1"
                     /translation="MENQNESLIKQYVKRRAKRRLFLWVFGTSAGLITILITVFVTLF
                     LILAAGSIDNSDSSSGGEAFTGEYSEGLPIYKEIKGRGPFSDEIAQYAVGAAVKYKLL
                     PSVILSQYGYESAFGTSASARNDLNYFGITWFDGCLFPKGTARGIGGIEGGWYMKFPN
                     SKAAFSYYGFMVATQSNFNACVGNKSPGASLLILGRGGYAAAGITEDSAYYKNCMSII
                     NTNKLMEYDEFAIKHWREGGGTGGTITGSWGNPFPGSSLDKNSFSGGQLFGKNPGGEF
                     RPNGFHDGLDFGSVDHPGSEIHAVHGGKVVYVGNPGISGLGACVIVINYDGLNMVYQE
                     FANSTGNSRVKVGDQVKVGQVIGIRDTAHLHLGFTRMDWRQAQGHAFIDDGTWIDPLP
                     FLNSSKK"
     gene            20518..21162
                     /locus_tag="ACG8KR_000806"
     CDS             20518..21162
                     /locus_tag="ACG8KR_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0200202.1"
                     /translation="MENKTLKIVLTAAISVSLIGNIALFWQLKEEQETNTYKIEEVAK
                     NASDKLKKNSSEAQAKIDKLNQTINEKNQEIDQLKQQDGTKAKEEVTEAQRKTAEEFG
                     QLAIDKSLSRNELTEKLKDVATQEVIDKLSPADDDHQHDDYGSYSFTLGDVQTYAQTS
                     SVKEEQNFVVFVDYTIGNPQFKDVKPQKIKGGLTVTEKQVDGQWKVTDFSYFTR"
     gene            21173..21979
                     /locus_tag="ACG8KR_000807"
     CDS             21173..21979
                     /locus_tag="ACG8KR_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0200203.1"
                     /translation="MSKKKGARQRFKKRKSYLRWLGIFMILGFLILASPIYTHKKVDI
                     PDEVSTNAFLNTKELTMVSKEKGANQQLVFEFAIDDEDQSLLKELANLNYRVEVKTAK
                     GDYQAIKAKVIKVSDDYFVVKLAHVPEEYIAMRLTIIPEKIDPKVDMQEPQDLIYYVH
                     EDKVKDQVKDEDYEQHAINYKVKGYKKEQKAAQKQMESFQATIDLNEELVKKLKDQLP
                     YQVADDQENTENKINSYQQEIETSKTQMKDQEEIIQKLQEKIDRMTKEKI"
     gene            22001..22231
                     /locus_tag="ACG8KR_000808"
     CDS             22001..22231
                     /locus_tag="ACG8KR_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0200204.1"
                     /translation="MKRTNLADKDTFAEQKVLIKAYLKVVKAKEICREVNLSNTTISE
                     LKNGRKNLDTLKFRSFEALYVAAKKYFTADNK"
     gene            22745..23053
                     /locus_tag="ACG8KR_000809"
     CDS             22745..23053
                     /locus_tag="ACG8KR_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0200205.1"
                     /translation="MEVRIRNVDEKIVREIDRRCDELSNRTGNKWSRNDYLKLLVEND
                     FDRPLMEYKQDKFDQLLNQLIDKFSFVQSHNTKVLEEYICQNNQIIELLIEQSRGGEV
                     "
     gene            23055..23477
                     /locus_tag="ACG8KR_000810"
     CDS             23055..23477
                     /locus_tag="ACG8KR_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0200206.1"
                     /translation="MAKVIDKHIYFYETHIDVLDEIIATHAEVKNYSEAVRFLCMNYD
                     SEVEKKVNSNQAKLNAMGKEISILTEMVSEFADLHLKDTGILIGTNSTVYQEAKERVE
                     GKIKANQTKKYSPKIKKTISESVTTKSEVFDPTRFLKR"
     gene            23497..25086
                     /gene="mobP2"
                     /locus_tag="ACG8KR_000811"
     CDS             23497..25086
                     /gene="mobP2"
                     /locus_tag="ACG8KR_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobP2 family relaxase"
                     /protein_id="HFQ0200207.1"
                     /translation="MSKSPAVILTCQFTLPNKKSFSTYIDYMTREKALEEKERRTPEE
                     EQELRLIKNALEDFYIPEGETFSESKTGKEFSETSNEAQSLMKHGINFDKLEEQDFTK
                     YLSYMTRHYALEQKKVLTHSEQKEYQRLNQAIKKYQTEPHKKASGNLPGVFSMASDEV
                     KGSDLKEIKQTFQRGQQKGSIIYQDVVSHDHSYLEKIGLYDPKTDTLDEEGLKAAGRK
                     MMETLFEKEQLNETGYWMATIHRNTKHIHIHFAVVEKKNTRKPYTEIENGVSYIVPKG
                     KRRQATLDEMKTNYVHELERFSYEKNRMGMEKRNLLTRKSDLRNTLTKVVKEPVRYDR
                     HAMSLLTEVYKNLPKKRSEWHYGDENRTKLSPYTREKLDELTQYVLTKEPGYQEYIQI
                     SKALKEEAKSLYGESKRESKDAEKNALFDLKKRTGNAILTELKKQDSQIKPLIERMDQ
                     MQSIYNERSFRTLKQLPTNEYKYSYEEFMKRRKQFQQRVVTQRSLRQLNRTINAHEEK
                     YHASKAYEEQQRRIQMEQERQ"
     gene            25098..25433
                     /locus_tag="ACG8KR_000812"
     CDS             25098..25433
                     /locus_tag="ACG8KR_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0200208.1"
                     /translation="MKQYLDQWKVIEGSLREERIEQLPDCLEKEHLFQIREMLINEQF
                     DPNQFLVVEYPATGVYCCNHVKGEKYFIIQEYEGKLAPYYTTWEMNEEGINNFPCKSI
                     EESISLTEC"
     gene            25574..>25761
                     /locus_tag="ACG8KR_000813"
     CDS             25574..>25761
                     /locus_tag="ACG8KR_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0200209.1"
                     /translation="MAYSTIGVVVEKSRDNLVFVTEIQTGRAFVVTDKAAKAYQSGDI
                     LTLNLTTKTFVDAAEDYP"
BASE COUNT         9601 a         4003 c         5302 g         6855 t
ORIGIN      
        1 agagattggc gcagcgaatc cttgataatg agctcggatg tgctaacgct agcgaggcag
       61 tctaacttta actgttagct cctgccgaag atcgtcacat ccgaggaggc tcattgtcaa
      121 ggacaatcgg aatagcatac ggaaagaagt tctgcacctt ataaaatttg tcaaaaaaac
      181 tgttgccatt ccatagatca aataaacaag aaccgtagga actacgggga tagcttggta
      241 aataagaggc acctctgtta gtaaagtaat acgctatcaa gtatgctctc ttcccaagaa
      301 gctcccactt caagcgtaag aaaccgttag gtttagctaa gtggtgagta gttcacaatg
      361 acgcttacga atgataatgg agacgaatta tctgttgaag atacttggtc atatttgagt
      421 gattatattg tcagcgttga aatcattgaa tttgtagcgg ataaagaaga ataaaaactg
      481 gaggaataaa ttttacagat aaaggcttag aagaagtcac gacgaataat aaaaaggtag
      541 tattacctta ggcattgagt aaggaattca tttaggagga aaattagatg gcgcgaccag
      601 ttttagaggc gatggatatg gatactttga caaaaggaag gtatgtacag ctgttagctc
      661 gtggttattc acctaaagat gtattcaaat ccctgtctaa aggaacagat atggaaaaag
      721 aaagattacg aaaagaattc gattattgga gaacccataa tggaatcaag gatttgaaac
      781 cagcacggcc agtaattaaa cgaaagaaaa agataaccca agactgaagt gtagagcgta
      841 tgtgatcata ggctttttta tttttgcaaa aaacaaggaa ggtaggaaaa ctcatgaatt
      901 atgaagaaat cgaaaacaga aaaaaagtgt cgaaataaat ggaagaaaag ttgctaaaaa
      961 cgatgaaaca aaaacacctt aaaagattat ctgtcatgca atatatcaat gatatgcaga
     1021 taacaggaaa agagaaagct tgtttgttag gttcaatgaa aaactttgaa cagttgagac
     1081 gaacttatgt gaaaacaagt tctaattgtc aactattgtt agaagtctca tgacattcat
     1141 taatagaatt catttttaaa actttttcta tggaaatgag aaaaagattt tttgttttgt
     1201 ctgaaagggg gtagctggat gagaaaatgg ttatttggtt ttttaggagt cgctttaatc
     1261 gtcttttgca gtgtgtttgg ctatgttagc taccaaaaac atgaaggaga ggtgtttaag
     1321 caaaatatag aaaaaaagat gcccgtagat caaatcaatg cacatgcaaa gagttataag
     1381 gaggatgcaa cgaacgtcaa taatgatatg tctttggggc aaatgttgtc catacaacaa
     1441 gaagcaatcg aaatgggcgt caataaacaa gtatttgcac aaattcagat tcctgcactt
     1501 ggtttagcat tgccaatttt taaaggggca aatcaataca ctttgagtct tggagcggct
     1561 acttattttt atgaagatgc tgaaatgggc aaaggaaact atgtcctagc aggtcataac
     1621 atggaaatgc caggtgtttt attttctgat attcaaaagt tgtcattagg ggaggtgatg
     1681 gacttagtta gtaacgatgg cgtctatcgt tacaaggtaa cgcgtaaatt tatcgtacct
     1741 gagtacttta aattaatcga tggcgttcct gaagagaact ctttcttgtc tttgccaaag
     1801 aaaggagaga aacccttatt aacgttgttt acttgtgttt atacgtcaca aggaaaagaa
     1861 agatacgtgg tacagggaga actacaataa caaggtttta atcaacgtga gtcgttgaaa
     1921 tatgaaatga ggagagtaaa aaatggcaat aaatcggtat gtaaaaaaag ctggaatgct
     1981 tattggaatg ttaggaatca ttgcaggctg ctttcaggga tatcgagcat atgcagaaga
     2041 tgtcacgcaa aaaacgccac cagagaaggt caatatcacg gttcataaat taatgtacga
     2101 tcaaggaacg caattgaatg ttgatattga cgggatcaaa aacgatggtt atacgcacga
     2161 tcaatatcct acaggtgtaa cgaaatacaa caaggcagat tatggtgatg ttgagtttac
     2221 gttaggaaat attacggatc aagtattacc gagagaagat tctgatctaa ccaacgcgaa
     2281 agtggacgag attgtacaag atgtagaaga caagggatct aacagtgagt acgtcaagaa
     2341 tgcaaccaac aaaattactt cagctgtaga tgaaaatggt gagatcactt ttactgatca
     2401 accagcctat gtcaattcca aagggaatgt atatgtggtt tatgaaagta aatccgctgc
     2461 tggattagtc acacaaaaag caaaaccaat ggttgtgatt gcgccaatga cagataaaac
     2521 aggatcagga tttttaaaaa atattcattt gtatcctaag aacattgttt ctaagttgag
     2581 ttttgaatta acgaaatttg gtgacgatgg cgcagaccaa agcaaacaaa cccttttaaa
     2641 aggggcaaaa tttgagttat acaagggcga acctggaaaa ggaacaaaat tgggtgatct
     2701 tgtatcggat gatcaaggaa agctgactgc gactgatttg acgttaggaa aatactattt
     2761 tgtggaagtt ccttcggaag ttgtagtggg atcagatcaa gaaccaacag cagatcaata
     2821 tttattaggc gcagacgctc gcaacgatgc gcacaataaa ttaacctttg aaattacgaa
     2881 tgatggcgtg acaagtgact taaaagcttc atacgtaaac tataaagcac cagtgatcga
     2941 taaaacagta acaaatggaa caggacaaga acattctttc caaattggtg acgcagtaaa
     3001 ttatcaagga acgattcata ttccaacaga cattgcaggt ggcgcagacg gtatcacagt
     3061 aaacggtgtg aaaagtgaaa cttctcctta cagtgtattt aaatggggag atacagcagg
     3121 acagggactt tcttatgtcg ctgctaaagc aaatatcaaa gtaacaaata aagatggatc
     3181 tgtggtatta aaagaaaata cagattataa aatccaaaat tcagaaaatg gctttgtgat
     3241 cgattttatc gtgaataatg gccaagtaag tgatactgta gcaagtttac acggacaaga
     3301 tcttcaaatg acttataaca tgtatgtaaa tgacagcgca gctgttgcca atccattaac
     3361 aaatagcgtg gatttcgttt ataacaacaa tccatttaat caggaagaac accacgaaaa
     3421 aacgaaagct gacgttgtga cttatggtgc aaaattctta aaagtcgata gcggactatt
     3481 tggaacagga atcaaagcaa caccgcttga aggcgcagaa tttgctgcta aaaacgcaga
     3541 aggaaaatat tatggtggat tagttgatac ggataaagac ggtgtcaaag aagcagtatg
     3601 ggtagacgat gtagcaaatg cagcgatcct aaaatcagat aaagaaggtc actttgaaat
     3661 cactggttta actgaaggtg aatattcact ggaagaaaca aaagcgcctg aaaactatca
     3721 aaaattaacg aaagagatct ctttcaaagt agacaaagat tcatacaaag aagaaaaccg
     3781 aatcacgatc aaaaacaatc aaaaagcttc cgtaccaatg accggtagca acggttttca
     3841 aacgtatgta ttaatctctt gcttgttgct aggagcaggg gcactttctg cagttgttta
     3901 ctttaagaag aaagcctaga aggatcaaaa ccaatgatta tcaggaagcc taagaaaaag
     3961 cgaatcatgg gtaaatggat cattgctttc tggttacttt cagctgtagg tgtcttactt
     4021 ttgatgcctg ctgaagcgag cgtggcgaaa taccaacaaa atcagcagat tgccgcaatc
     4081 gatcgaacag gaacggcagc agaaacggat tcttctttgg atgttgctaa gattgagtta
     4141 ggcgatcctg ttggtattct tacaattcta agtatttctt taaaactacc aatttatgac
     4201 ggaacgtccg acaaaatttt agaaaatggt gtaggtatta cggaaggaac tggtgatatt
     4261 acaggaggaa acggtaaaaa tcctttaatc gcaggtcata gtggacttta taaagataat
     4321 ctttttgatg atttaccttc agtaaaaaag ggagaaaagt tttatatcaa agttgatggt
     4381 gagcagcacg cttatcaaat cgatcggatt gaggaagtgc aaaaagacga actacaaaga
     4441 aactttgtca cttacttaga atcaaatccc aatgaagatc gagtaacgct aatgacatgt
     4501 acgcctaagg ggatcaacac acaccgtttt ctagtgtatg ggaagcgagt aacctttaca
     4561 aaatcagaat taaaagatga agaaaataag aaacaaaaat tatcttggaa atggctttta
     4621 ggaagtactg tttttctttc agtcatgatc attggttcgc tatttgttta taaaaagaaa
     4681 aaataaagga gggtaaccaa catgagaaat agaaaatggc tatttgtttg tgcagccgct
     4741 cttttttctt tcactgccta cacacagttg acgtttgcag acagacaaga aagcacggat
     4801 agcacagaac aaacaatcca aacgtcagat agcagcacga ttcaaagcag cgatgaaacc
     4861 gtcgattcgt caacagttga ttcttcagaa gaacctaaaa ttgctgaaga cgatcaaata
     4921 gtcaaacaga ccattcacgt tcaaaagatt atttcttcag aacaattaga caaccagggg
     4981 catgaaaaac aagaaggagt aaatggcgcg aagttttctg tttatgatgt atcggacatt
     5041 ttacaaaaga tggatgttaa ggatctcact actgaccaaa ttgaatccca attaaaaaat
     5101 cgagtgaaaa aactatccag tgatcaattg aagctagttt ctaatgaaga aacaaagaca
     5161 attgatcaac aaacaggtgt ctttgaattc tcggtagaag tacaggcaaa tcagaaacaa
     5221 gcttattata ttgtcaatga atcaagtcct gaaaacattt ctaatagcga ggatattttg
     5281 ctattaacac ctgtttctga taaaaacggt gaattcttaa aggatgtgtg gatctatcct
     5341 aaatcagaag cttcgcaacc taaagaagaa gtaaaaaaaa tcgtttctac tggcgtaaag
     5401 aaaaacttct ttgagaattg ttgggatttt gttactcact tgttttctag agattaagga
     5461 agaaataaaa tgaaactgat caaattatta ttattcaatt tttcttgttt aaccgcagtc
     5521 tttttgtact gcggtttatt attttctaaa ctatctattt tgccaagcta ccaactatac
     5581 ctaatgagga ttttaaacat accagctgct tccatgccgc tagatattat ggaaaagctc
     5641 attaggaatc cattatttac tacgatcaat ttgatcgtta ttgtcggaat ttcactagtc
     5701 atttataaaa aagaggggat gaagggatga attttaaaca ctcgtggaca agacggttaa
     5761 aatatgtttt tcccataatg atgatgttag gtacattgtt tgggttaaaa acagccaatg
     5821 tctttgctga aaaagttatt gtcgatccgt caaatccaat ccagaacgta aagaataatt
     5881 ttattattcc aaaagatgtt cctaatgcaa aaacaaatat tactgtaaac ggagcgtcaa
     5941 aagtttacaa atatcatgtt gatgccaaca aaggtggttt tacagacgac tacgtaggat
     6001 tagtggaggg aaacaaaaat atacgctatc cttctttcca aaaagaatac ggtgaaacaa
     6061 accttgtttt agaaggcgta acaaccaaca ccaaagtcaa tattgactat ggaaaaatcg
     6121 gtacgtataa cgggaaaaaa gtcaatatca agctagttct atctaatatt cacttatact
     6181 cggatacgtt gccgtggaat ctactagata acaactatac aaaaactcat tttcgagacg
     6241 atggctataa aaatacgaat ggtgcaatga gtaaatctaa aaaaagaaca gtactttgga
     6301 tctcagacaa tcttttctca ggaattgttt accattcaac acaaatgaat gtgcagctag
     6361 tagccacata tgaggacggt agccccgttc aattttcagg agatacattt atcagtttta
     6421 actctttgaa ccctgcagga ggaaaaagca cggatcttaa aggagaatat gcccactatg
     6481 acaaaatgaa tacgacagat tggtatgtca gaaaagatac ggttctttca gaatttaaat
     6541 ctttctacaa caatctgaac gtggtaggtg gccacccagg aggaagctct aagctgacac
     6601 aagcggataa tgatttcaat aatttgcatg ataaattagg agatccgaag tttggtcaag
     6661 gtactgtgtc ctttaaaata tctgaagcaa atccgacgtt tgtgattggt tcttctaatg
     6721 ttcaaacctg gtttacttta tcttctgcga caatctttag tgttgttcct gatcaacctg
     6781 aaaaaacagg ggttgataaa aatggaaata acgtgaacga taaaatgttg caagtaggag
     6841 acacgattca atatcgaatc aaacagaagg tgaatcgatt aggagtggat ttactagcgg
     6901 tttacgatcg atttgagtta atcgataatt taccaaaaga agtaaattac gtcgatgcac
     6961 acgttgaaag tggtacaaat aaaaaattcg atgtatctgg tgaagtgacc tatgacaaaa
     7021 caaaacacca ggtaaaatat gcagcgaaag ctgatacatt aaaaaatcga atgaagtata
     7081 acggcgaaac ttacgaatta gtgatcaatg taaaagtcaa tgaattggcc aatcaaaata
     7141 gtgtagccaa aaatcaggga acgtcgatca ttaataaagt agaaaaggat acaaatatca
     7201 ttaccgttta tttccctaaa attccagtga aagaagtaca acaaaatgga aaagatgtaa
     7261 acggacgaaa tgacggtaag aagggtacgc cgacagctcc tttaaacgca ggatctgaag
     7321 tacaatatct agtcacacaa aaatggcaca caaaaggcgt agacgctgcg tcagatcact
     7381 acaaacaatt tagtattcaa gatccaattg aagctcgttt gacatataaa gaaggttctg
     7441 cgcaagtaat agataagagt aaaggaaaag atattacttc tgaaggcaca ctaacctatg
     7501 atagcaattc aagaacgctg aagtgggaag cttccgctga cttcttaagt aacaatcttt
     7561 tagatggacg agaaattcag ctgatattta cagctaaaac tccattacaa tcagaaaaaa
     7621 atattgataa ccaagccgta gtagcagtag ataatgtttc taataaaaca aatgttgtga
     7681 caattggggt tgatcctaac ttgccacagg tcattgttcc taaaacaggt tctacacatt
     7741 tagtaacgat ttcagcggtc agcttactat tacttgtgtt agcaactttt agttatgcgg
     7801 ttctgagata catttaaaat aaaaaaacga aagaatgaat aaaatgaaaa agttaataat
     7861 agtagttact tccttgattg ccatgattgt tattttagtc ggatgtagta atgaaaaaaa
     7921 taaacaaact aatcaagata atggcgtatt gaaatcagga actatgtgga aagaagaagt
     7981 aggtggtctt gtttataatt tgaaaattat agacgagact acctgggaat actctgagag
     8041 tgtatggcat ccagatcctg tacaaataac agtcaagagg caaaaagact ataaaggttt
     8101 agaaagatac aaaatagtag attcagctgg tgttagggaa tttataaaca aatctgattc
     8161 tttatttatt gttgttccct atgagaagaa tggcgtaaaa aaaataattt ttcttggatc
     8221 tagcaaggat gaaaagcgaa cgaaagagaa attgattcac gatggatcac aatccaataa
     8281 atataagtta caaaagacta gcgagtgatt gctagtcttt ttatttgtcc tgaaggagga
     8341 atcacagtgg caaaagctaa aacgccattt gaaaaagccg cgtctaaaat cttagaacgc
     8401 cgcggtgtat ctgaaacaca gtggaaacaa gaagtccttg aaaaagcaca atttgaccta
     8461 ttacggggaa aagacaagga cttagaagga tatatctatc aacgtgaacg tgaaaagctt
     8521 gttttcgatg aaattattaa acactaaaag gagcgaacaa ttttatgttt gaatcaatta
     8581 caatgaattt tttaacacaa ccgaaagtag tagctgcggt tgatattttt agagcattac
     8641 aaactgccgc aactagttta aaaaacaatc tgatgatttt tgctgctgct gcggccgttg
     8701 cttccattgt tattgggggt cttttcttcc tatttggacg aagaggagct gaaaatggga
     8761 agatgcacgt aggctatgta ttgatggggg taggagttat tttagcagca gcttctattg
     8821 tggtttcgtt atttggattg tttggacaaa cacctcaagg attataagaa agtaaaaaag
     8881 taggagtaag aagtagtcga tactttttac tcctttttta tgaaaggaga atactcatgt
     8941 actttacgat tgcaggcaat accaaaacag ataaagagtt aataaaaaaa gcaacgaagg
     9001 aagtaggaaa gcgtacttct tttttaccga aaacaattaa atattctgtc attgaaacgg
     9061 aagaacagct acatgtgtta gggaaagtac tggaacacga aagtcgatct tctgttaagt
     9121 tgactctcac gttacatgac acagaaggaa aaatagagaa agctggtaca gaatacgttt
     9181 ttggcccact ggattatcaa atttcagaag gtttctatcc tacattgatg gatttaatcc
     9241 gccttgatgt gtttaaagaa acggatgaaa ctatgagtgt ggaagagtct tatcgaaacg
     9301 aaaaactttt acaagtttta gaagaagagt tagagagcga ccaaagaaaa gtgaatcaag
     9361 ggagtgaagt cgaagtaaat gaagaagtac catgggaacg accaatattt ccaaagaagg
     9421 agtcaaaaga aaaagagaca gtctatgttg acgaacaagc tgatattccg actcagaaat
     9481 cgatatttga ggaagagaac gatgtgatgg gtcaattgga ggtagatccc tttgaagaaa
     9541 caatggagga tgaagagacg gccgaagtag aagttgatcc agttgaagcg aatgaagagt
     9601 caacatggac tgaaggggat tctcaggaat atattgaacc acttatcagt gaagaagtag
     9661 aaaaaaacga aatagagata caaaaggcaa ctgtatttga ttatattttg gatcaatatc
     9721 cgccagagca tgaatggatg aaagaaaaaa acaaattatt tttaaatgag ctttattcga
     9781 aatatcaatt accccaaacc tatcaagcat ttttagagag taaagaagaa ttaatctcac
     9841 agggtaaagg cacactttta gaacagttag agtctgtcca atcaacaaat tggcatgagc
     9901 ttgcacaaga agatttatta gccacctttg aagaacgtca acaagaaaca gatcaagaaa
     9961 ttaatgagta tgcgacggaa cagtcgtcta attgggagaa agcaaaagag gaactaaata
    10021 aagaagaaca gcgagtgatt gaagaagaag tacaaaaaat taaagagcga tacgaaaaga
    10081 aacgcgacaa tgcgtattcc ttgtgtgtag caaagatcaa taattttact cagacgaatc
    10141 aagaagcgtt atcgaaagaa aaagaaaact tgttaaaaga acgtattaaa gaacgaaaag
    10201 aagaatttta ccgaggtttg cgtacagata agctaaagat tagcaaagaa ttaaatgaac
    10261 aactgaataa gctatatcaa acaacgactg aagttcttat ggaaaaatac gaaaagatcc
    10321 agcaagaatt acaagtgcaa attccaaaat ggaaaaaaga gtatgaaatt caacaacaag
    10381 aattgaaaga acaagaagaa aaacaacggc aaaaagaaaa agagcgcgaa caattagaac
    10441 taaaaaaacg tgaacaggat cttgcggaac aacaaatggc aatagaaaaa caacggttaa
    10501 accaagaaaa ggaaaaagaa gcagctgctc gtaaggaacg tcaagaacaa atggatacat
    10561 tgcgacaagc tcagttaaat tatctagcac aacctcaacc aacgccttca gtagctgctg
    10621 ttcaaacccc aatgaacaac caaacaaatc taactccttc accacagcgt tcctttaacg
    10681 gatggatggt gggatgtatt gcaagtttaa gcttgcttct aggtggagga acagtatttg
    10741 cttttaatca tatcaatcaa tcttcacagg ctgattctgt ggccacaacg acgtcaatca
    10801 aggaagaagc ccaagaacaa gcaagaaaag agtatgaaca acaattagaa gaattattga
    10861 atcgtaccaa tcagtcaaca acagagtcaa gcatggaaaa tagcaaagag agtgattcta
    10921 gtaaggctaa agaaggatca agcgattcaa ctgtacaatc aaacacttcc gaaagttcag
    10981 aatcaaatga ggtgaaataa tgcgtgttag agaaacaacg aagcgaaaag acaaacgatt
    11041 caatttagaa aagaataggt atctttctca tttctatcgc tatactccct caggagaaga
    11101 gctaccagaa aaacatgaca aacgcatttc gaaaaagatt ttttatagtg gacttgtata
    11161 cctcccttta gagtatttag taacaagagt accagaagtt ttcaaccgat tggaaattgt
    11221 taagggaagc ttacaaggaa taaatctgtt tcaattacag gaaatgccac aggtctttca
    11281 ttttgattca gctggttttt ggaatccatt tactcctaca actagtttgg gtacacgttt
    11341 agcaatttcg acagtttgtg tggttccagc tgtacttatg ggattgaaaa tggacatgtg
    11401 gtttcgtcct ctagcagatg gacaaaaagg ggataatcga ttaatgacct taaaagaaat
    11461 ccaacagatt tatccgcaaa tacctgaaac aaaaactact tttccaggtt atggaggtat
    11521 tccaatcaca cattacaaaa aatattggta cattcaaaca gatactgtta atacgtgtat
    11581 tgttggtacc tctcgatcag ggaaaggtca gattgaagtg ttagccacca ttgacaattt
    11641 atctcgtgca gaaaaacaaa gttcaatggt cgttaatgat ccaaaaacag aattatatgt
    11701 tggtagtaaa gatgaactag aagctagagg gtatgatgta tatgccttta atatcaccga
    11761 tccactgcaa tcaatgagtg ataatccatt ggcactcatc gtgaaatact ggaaacgagg
    11821 agacatagat actgcaacac agttaaccaa tactttcaca aatacgattt attatgatgc
    11881 caacgcttca gataacaaat actttaacga aaacgcgcag aaagctgtca atgcgcttat
    11941 ttttgcgttg ttagaacaag ctgatcaaac aggagattac tccaaattga caccgaataa
    12001 tctcgttgag cttctttctg agattggcgg ttttaattat tatccagatc cagacaatga
    12061 attcaaacag ttgaatgcct tagatgaatt tatgaatcag ttgccacctg gaaacattgc
    12121 aaaaaaacaa tatggggcaa cgaaaattgg atctgataaa gcgaagggaa atattttatc
    12181 aacggcaatc acgggattga atccatttac tctaacgaaa attgcaaaga tgacttctca
    12241 atcaacgatt gattataaat cggttgggtt tccaaaatac ttagatttga aattacatga
    12301 aagtctttta aatcaacgag tcgtagtaga attttatcat aagggaaaga aatttcatga
    12361 agaacgtgtc aaagtaggct ataaaggatt ttgtgaaatc aattttgatt gtcatttagt
    12421 tgcaggtgat caagtttgtt taaaatacga tcatcaagga aaagaatatg tggcttggta
    12481 tgaattttct caaagagtat tgaaagacga agagggtaat atcgtttatc ggaaaaaacg
    12541 aggagaaacc catttgccag aattagaaaa agaagccgtt ctaacattag atgaggagaa
    12601 aagtaactta ttagtagagt ctattcggat gcactatacc gataaaccaa tggctatttt
    12661 tatgcttaca cctgattatg atccatctaa ccatatcatt gttagtattt ttcttagtca
    12721 gttatataaa gaattatcta cgcaatgtgt taagactaaa ggagataaat gtcaccgcag
    12781 gattcatttt attttagatg aatttgggaa tatgccacca atggataata tggaaggaat
    12841 catgacggtc acagctggcc gaaacatgtt gtgggatctg tttattcaat cttatcagca
    12901 actttatgcg aagtatggag aacaaaacgc agcgattatc aaagaaaatt gtcagacaca
    12961 tatttatatt atgtcgacag atgataatac catcgaagaa ttttctaaaa aagtggggaa
    13021 taagacggtt gaacaagaga acagtaccat caattcatta ggtatgaata ctcatatcaa
    13081 tcgcaatgtt gattcagatc gtatcttaac tcctgaaaga atttctactt ttttagaagg
    13141 tgaaacgatt gtactcgctc ctttaaaacg acaagatttg aaaggtcata aaatacggcc
    13201 gtatcctatt tttaatcatg gggaaacgaa tcttccttat gcttatcaat atattgaaga
    13261 tttcaatcca tcgtgggata tcaatgatat agacatacat tgtacacatg cggacttgga
    13321 tcttaaggct ttacaaattg attatgatta tttcctacca gatgaatatg ctagaaatct
    13381 ctatcatgga ctgcatgatc aattagaaga aaaaaacgaa gaaaatatgt tttataaaat
    13441 cgttcaatct cttgagaatg agacggctaa aaaattgtta attcaatttt acgaagaaca
    13501 agcagcagat cgacttcaag aatatgttga aaagttgatc aaaaataaag aaatttctga
    13561 aatgattggg aaacggttgt tattggaatc tgaaaaggtg gtgaaagaat gatcattgga
    13621 ggaatttttt ctggcatagc cgactggtgg tcaggcttat tcggagaagg ggatgcaaac
    13681 cccaaaaccg taacgaaagt cttacaagat tatggtaatt atctgcagta tcaagatgta
    13741 tttagttgga tttggaacac ttttctgtgg ggattgatta aaggattata taatctcaat
    13801 actttactag aaaaaaccat ttatcaaagc tttgatttaa aagacttatt gaacgccgct
    13861 ggggtaaatg cactatttca aagtttgatt agtaaggtat tggctctttt ttgtgtcgtc
    13921 acattaattt atttaggctt gaaatttttt atgagtaaac acccaccaaa agtcaaaaat
    13981 atcttggtta atttatttat ggccatgatc ttgatttttg gtgggagttc tttaatcgat
    14041 gaagggttaa atatcagtaa aagcttttac ggagacgtca ctgcagcaaa taaagcagat
    14101 aaaacgggtt cacctgcttt tcaaattatt caaaataatg tgatcgatgt cagtacatta
    14161 ctagcaactg acccgaataa agtagaatcc ctgccaacag acaaaagaaa tgctttaaca
    14221 gcgaaaaatt ttaaaacagc gaatatcaat gggattatta ctcctaaaca agctaaagaa
    14281 atggcggata aagataagag tggtttatca gatttacaaa aggatcggtt aagagcgtta
    14341 caataccgat tggaactaga tgataatggg aaagaaattc ctgttcaaat tagtgacgaa
    14401 ggcttagcaa aatatgtcta tacttcaggg tatcgtcgat acagtagtaa accaggtatt
    14461 atcttagctg gtgaatcttc tttggcggtg gcctatttat ttatcctatt tacgatcgtt
    14521 acttgcatta ttgagctggt atttaaaaaa ttctatttag tgattgccgc gtcaacggac
    14581 tttgagacag gccaacggat gaaaagtgcg atagaagata tttctcaatc cttcttgtta
    14641 ttggcgttta cgattttaga attacgaatc tatacattaa tgctttcagg tatcggtgat
    14701 ttgcatgcgg caggaaagat caatggattc ttgtatgttg ttgctttaat tgtgctaact
    14761 attgcgctat tcaaaggctc tcaatcggtt acgaagattt tcggtgttga tacgagtctg
    14821 aaaaacggca gtaattctct tcttagcatg tttgccttag gaaatattgc gaaaaatgtg
    14881 ggtagcggag ctaaaaacct tgcaagtgct ggtaagagtg gtttaagcca tttaaatgat
    14941 attcgtaaaa atggatttgg ccatcgaaaa gatgttaccc ctgataacgg ggaagaacaa
    15001 tcagacgata aagctacaac tgtacctaat agtaagaaaa cagtttccga aatgttcggg
    15061 aaagcgaaaa atggtgtttc taaagccgca gaaggtcttg gttacgtcaa tgaacgtggg
    15121 ttaaaaggaa cagctgaaga tcttacagaa aaagccaaag acaccttaga agagaaatta
    15181 gacggcacga gagctaaaga attggctgaa gctgtgaaga atccatccga agcactagaa
    15241 aaacaaaaag aaaaagcttc agaagtcaaa gaaggactaa aagatgcgta tggtgaagga
    15301 caagttaatg cagcagtaaa agctaataca acaaccgaaa aagaaaatca agaaggtata
    15361 gtgattcctt cagtcaaaga tcaccaagaa gcgttgcact ctaatgaaaa ttcgattgaa
    15421 gagaaaaatt ccatagaaga aagaaattca gaagaaggaa aaaatggaca gttaccaaaa
    15481 actgatccag aacaatatcg ctataaacct aacagtgacc ttgaaaatga gaagcgaatt
    15541 tcagatttat caaaagtaaa tcaagaagac aaagcagaaa atgagggaca gttaccaaaa
    15601 actgatccag aacaatatcg gtatagatct gttaactccg aaaatgaaaa acgcatttca
    15661 gatttaccaa aacgaaatca agaagtacaa acggatgagg attccatcgt tctgccaaat
    15721 gataatggaa tcgaagttgg aactgaaaaa tcaacgaaaa ttggtttacc taaggcacaa
    15781 aatttaccaa tgaaagatag tcgtttgaat caattcaagg agaatgatca aagctttgtt
    15841 caccaatcaa ctgcaggtac tgttcaaatg aaaccaagtc gtttgcctga aaatggaaat
    15901 ctgacaactg tttcttacaa tttatctgaa agtggtgaga cagggaatgc gtccgttcaa
    15961 aatgttgtga caagagaagc tgcaccaaaa cctattcaaa acgttcatac aggttacaag
    16021 ccagcaaatg tggcatttac tgcaccaaca aaaataaatg tgacaagaga aactaaaaac
    16081 aggaacaatg cattgaaaga acaactattg agggatatca aacaggatat caaaccataa
    16141 aaggcctaca agttaggcct tttatttata tgaaaaggag aaatcaatta tggataaata
    16201 tataaacatg aaaaaccctc caagatttag tggagaatgg aagtttttcg cttttttctt
    16261 tttagatcta ttaacgcttg gtggctgctt tttttgggcg aaaatggtca ataatcttgt
    16321 ccacttacca ctcttttttg cactcttgaa ttatggctta tggattgcga tagcgttagt
    16381 gattgtttgg cgaccctata ataacccagg tcaacgacaa atttttgcga ttatcgagtc
    16441 tatttttttt gcggacaaga accattatca tgcgttagat cctaaccgaa attatcatgg
    16501 tacgtataag gaggatgaat aaatgcgagt caaaggacaa aattcagttg aaaggaaatc
    16561 cattttgcag tttggaaaac cagaagagcg ctctatcttg gacttatttc cgtataaatc
    16621 ctgtacagaa gagggagtct taattactag agaagatcat tttcaacgtt attatcgtgt
    16681 agtttctaca gacgtggaag gattaaatga acaagagaaa ctagaacgaa tgaatcagtt
    16741 gaccaccgtc atgcgtacgt atgtgccaac aattaagtta gttagtttga ccacagagac
    16801 ggatctaacg gaacaaatcg tgcaaaagcg gcaattatta gataaaaatc gtatggaaca
    16861 gtccttagga agaaatctac aacgattaaa aaaatatgaa aagaaattag tcgaagaaat
    16921 ccaggaattg aaacgagcag aacaagaacg tcctgactta agcttctttt tcgtcattga
    16981 aggaaagaca atcaaggatt tacaaaccag aaaccgtcag ttaatgcgat ctagtggcat
    17041 tttagggtta aaaccactct acaaaaaaga attgatcaag attctgtatc gaatgaacaa
    17101 tatgaatgat gagtaggaat ggagaagaga gaatgaaata tccgttgata tggttagaaa
    17161 aagatgttcc gaaggaagag accactagaa aatttcctca cactttaagc ctgggtaagt
    17221 tgggaaaagg gcaatctttt aaccaagtaa agaatcaaac aagaagaggt gactgtacat
    17281 gttagaagca attaaaacag ctttttcacc acaaaatgat caaaaagtac ttgatccatt
    17341 aaagcaacta ggaaatcggg aaatagaaat tttaaaatcc aaagggtatg atttagattt
    17401 tttaaaacaa gtgatgccac aaggtgggtt aaattttgat gaagaatatg tgacgtatgg
    17461 taatggttat tcacgatgtg tgacagttta tcactacaca aaaaatccta cgcttttttg
    17521 gttggccaat ctcatgaaca atcctcatac agtggccaca ctcgatgtta gcacagatga
    17581 aaagcaaaaa gtgattgatc aaatcaatcg tgccttagat gaattaaatg atcgatcaga
    17641 taatgaacga aaagcgactg accgaaataa aagcgcagaa gaatacctgg aactagcgga
    17701 atatgctcgt aaattgactt ctaatgggga aatttctaag caagtcaaag tgagagtaca
    17761 tgtttacgga aacacgattc aagaagtaga tgaacgagcg caggaactga tcgaaaatct
    17821 aaaagggatg gattatcgtg cggtgactta tctgcataaa accaaacgag attggcaatc
    17881 gatgtttacc atgttcgatg aacaagctga atggttcgga aatatcaaag ggcaatcact
    17941 accatcgtta aacattggcg gaggtttccc gttccaccac caaacgctaa aagatccagg
    18001 agggacacaa tatggggaaa ccatgacagg cggcccattt gtctggaatg cgacgtataa
    18061 aaatagtgta cggttgtcta ctaatacctt gcttctagga atgatgggag ctggtaaatc
    18121 gaccgttctt aaaatgatcg cggaagccca tttagctgca ggagacttta tttggggctt
    18181 tgaaaaggga aaagacttta ttcctttctt aaaggaatat aacggtatta tggtgcgttt
    18241 agacggatct gacggaatga tcaatccttt agagatattt gccacacgga cgtatgatga
    18301 agcatccagt ttatatgacg atggatcagt caaagatttg aagatcaatg aagcagcgtc
    18361 ctatcaaaca cacttagaca aagtggtcta tcaggtacag ttagtgagtc ctcagctaaa
    18421 aggaacgatg aaagcagaat ttaagaccta cttaaatcgt ttttatgaag agtatggaac
    18481 agtaccacgc ggctttactt cctcaaattc gcgttcaaat acggagacgc aagtcacagg
    18541 taaagatcca gaagcttatc cgacgttcaa agaattttta gactttttag gacaactgga
    18601 attaccaggt gcttcccaag agaaaaagaa tcgaaaagaa gaaatggaat ccatcgtaga
    18661 atccttatgt gagacgtatg gcatgatttt tgatgggcat agtacgattc gacaccttga
    18721 ccaacaacag ttggtctttt ttgacattga ctctattaca ggcttagatc gaggggtaca
    18781 acaatgtcaa atgtacatgg ctatgacgtt gatttggaat caggcattga ttcaaggaag
    18841 aagacaacga cgtttaatta aagaaaaaca aatggaagaa caagacttca aacgcttctt
    18901 ggcgatgatt gatgagtgcc ataatgcgat taatccagat tttattgaaa cggtaaatta
    18961 tgtcacaaac tttcaacgag aaatgcgtaa agtagaagca atgactgttt tagctacaca
    19021 atccccacaa gaaatggtgc cagaaaatat gtcttctgat aacttctcga aactgaagaa
    19081 agtctttgaa ttttcttcta cgaagatctt tatgcgtatg gatgaatcta ttttacgtca
    19141 tatagaagga ttagtgggta aatcaatgac gaactcagaa ttagaatcca ttcctcaaca
    19201 aaatcaagga gacgctgtga tcaattttgg ctcaaaagaa accattcgtg ttcattttag
    19261 tccgaacaaa cgtcaattaa aattgtttga tggaggaaaa taatggaaaa ccaaaatgaa
    19321 agtttaatca aacagtatgt taagcgtcgt gcaaagcggc gcttattttt gtgggtgttt
    19381 ggtacttctg caggtttgat tactatattg atcactgtgt ttgtgaccct ttttctaata
    19441 cttgcggcag gttctattga taattctgat tcaagtagtg gtggcgaagc ttttacagga
    19501 gaatattcgg aaggcttacc catttataaa gaaatcaaag gaagaggccc tttttctgat
    19561 gagattgcgc aatatgctgt aggtgcagca gtgaagtata aattattacc aagtgtgatc
    19621 ctttcgcaat acggttatga atcagcgttt ggtacgtcgg cgtccgcaag aaatgatctc
    19681 aattattttg ggattacgtg gtttgatggg tgtctgtttc ctaaaggaac tgcgcgtgga
    19741 attggtggca ttgaaggtgg ctggtacatg aagtttccca actcaaaagc tgccttctcc
    19801 tattacggct ttatggtagc cacacaatca aattttaatg cttgtgtcgg aaataaaagc
    19861 cctggcgcaa gcttattgat cttaggacga ggagggtatg ccgccgcagg aattactgaa
    19921 gacagtgcct attataaaaa ttgtatgtct attattaata caaataaatt gatggagtac
    19981 gatgagtttg cgattaaaca ctggagagaa ggcggcggaa ctggtggtac gattacagga
    20041 agctggggca atccattccc aggaagtagc ttagataaaa attcgttcag tggaggacaa
    20101 ctttttggga aaaatccagg aggagaattc cgacctaacg ggttccatga tggactagac
    20161 tttggttcag tcgatcatcc aggaagtgag attcatgcgg ttcatggcgg aaaagtcgtg
    20221 tacgttggca atcctggtat ttcaggtttg ggtgcgtgtg tgatcgtgat taattacgat
    20281 ggcttgaata tggtgtatca agaatttgca aatagtacag gaaattctcg agtgaaagta
    20341 ggcgatcaag tgaaagttgg tcaagtaatt gggatacgag atacagcaca cttgcattta
    20401 ggatttactc gaatggattg gcgtcaagca caaggacatg cattcattga tgatggtaca
    20461 tggatcgatc cgttaccatt tttgaatagc agcaaaaaat agatcaggag aaaaaatatg
    20521 gaaaataaaa ccttaaaaat cgtcttaaca gctgcaatta gcgttagttt aatcggcaat
    20581 attgcgttgt tttggcagct taaagaagaa caagagacaa atacctataa aattgaagaa
    20641 gtggctaaaa acgcgtctga taagctcaaa aaaaattctt ctgaggcaca ggcgaagatc
    20701 gacaagttaa atcaaaccat caatgagaaa aaccaagaaa tcgatcagct gaagcaacaa
    20761 gatggcacaa aagcaaaaga ggaagtaact gaagcacaaa gaaagacggc agaagagttc
    20821 ggtcaattgg ccattgataa atcattaagc agaaatgaat taacggaaaa actaaaagat
    20881 gtcgcaaccc aagaagtgat cgacaaatta agtcctgcag acgatgatca ccaacatgac
    20941 gactacggaa gttactcttt tacattaggt gacgtgcaaa cttacgctca aacaagcagt
    21001 gtaaaagagg aacaaaattt tgtcgtattt gttgattata caatcggcaa tcctcaattt
    21061 aaagatgtca aaccacaaaa aataaaaggt ggattaactg tcactgaaaa acaagtagat
    21121 ggtcaatgga aagtcacaga ctttagttat ttcactcgtt aggaggaaga aaatgagtaa
    21181 gaaaaaagga gcaagacaac gttttaaaaa acgaaaaagt tatcttagat ggctggggat
    21241 ctttatgatt ctcggctttt tgattcttgc tagtccgatt tacacacata aaaaggtaga
    21301 cataccagat gaagtaagca cgaacgcatt tttaaacacg aaggaactga cgatggtttc
    21361 aaaagaaaaa ggagcgaacc agcaattagt ttttgaattt gcgatcgacg atgaagatca
    21421 gagcttatta aaagaactag ctaatcttaa ttatcgtgta gaagtaaaga cagctaaagg
    21481 ggattatcaa gcaataaaag ccaaagtgat caaggtgtct gatgattatt ttgtagtgaa
    21541 gttagcacat gtaccagaag aatatatcgc catgcgttta accattattc ctgaaaaaat
    21601 tgatccaaaa gtggatatgc aagaaccaca agatctgatt tattacgttc acgaagataa
    21661 ggttaaagat caagtgaaag atgaagatta tgaacaacat gcgatcaatt acaaagtgaa
    21721 aggatacaaa aaagaacaaa aagcagcaca aaaacaaatg gaatccttcc aagcaaccat
    21781 tgatttgaat gaagaattag tgaagaagct aaaagatcaa ttgccttatc aagtcgcaga
    21841 tgatcaggaa aatactgaaa acaagattaa tagctaccag caagaaattg aaacatcgaa
    21901 aacgcaaatg aaagatcaag aagagatcat tcaaaaactt caagaaaaaa ttgatcgtat
    21961 gacaaaagaa aagatttaga aggacaggtg gagtgaaaaa atgaagcgaa caaacttggc
    22021 tgacaaagat actttcgctg agcagaaagt attgattaaa gcctatctca aagttgtaaa
    22081 agctaaagaa atatgtcgag aagtgaattt atctaataca acaatatctg agttgaagaa
    22141 tggtcgaaag aatttagata ctttaaaatt cagatcattt gaagctctat atgtagctgc
    22201 taaaaaatat tttacagctg ataacaaata aaaaacagga gtattttatg tgaaaaaagt
    22261 atataaaaaa tgcttatgaa tcagctatat caccttaacc atttgttaat gagatgggag
    22321 aaaacattaa ccacgattct aacgcaaaca tcaagcacat aaaacattta acactcctcc
    22381 tttctggctg aagatttcaa ccaaacagga agacagcgta tcaattatag catacacttt
    22441 ttcttaaatt catttttcat atgacaaaat atttccttct aatcgacaaa gttagaagga
    22501 ttttttattg tctaaataca caaataacct taatttataa agagcataag gaaacgtggg
    22561 aacacttttt aaaaaaagtg ttccaatagg ctgtgcctat tacgtttctc aaaaataatt
    22621 gtgggaacaa aatatttaaa aataggatag tgaaaggata gcaaaggata gtaaaaggat
    22681 agaagcagga tagcgaaaag gataggctcc ttttcgctat cctttttaaa ttggaggaat
    22741 taatatggaa gtaagaataa gaaatgtaga tgagaaaatc gtaagagaaa ttgatcgaag
    22801 atgtgacgaa ctaagtaacc gaacgggtaa taaatggagt cgtaacgatt atttaaagct
    22861 actcgttgaa aatgattttg atcgtccttt gatggaatat aaacaagata aatttgatca
    22921 attattaaat cagttaatag ataaattttc ttttgttcaa agtcataata caaaagtatt
    22981 agaagaatat atttgtcaga ataatcaaat tatcgaactt ttaattgaac aaagtagagg
    23041 aggagaagtt taacatggcc aaagttatag acaagcatat ttatttttat gaaacacata
    23101 tagatgttct agatgaaatt attgcaactc atgcagaggt taaaaactat agtgaagccg
    23161 ttcggtttct atgtatgaat tatgattcag aagtggagaa aaaagtaaat tccaatcaag
    23221 caaagttgaa tgcaatggga aaagaaattt ctattttaac cgaaatggtt tcagagtttg
    23281 cggatctcca tttgaaagat acagggattt taattggtac aaattcaact gtttaccaag
    23341 aagcaaaaga gcgtgtggaa ggcaagataa aagcaaatca aacgaagaaa tattctccta
    23401 agataaagaa aacaataagc gaatcagtga ctacaaaaag tgaagtattt gatccaacaa
    23461 ggttcttaaa acgataaaaa ttagaaggag aatgtgatga gtaaatcacc tgcagtcatt
    23521 ctcacttgtc aatttacctt gcccaataaa aaatcttttt caacatacat tgattatatg
    23581 acaagagaaa aagcgctgga agaaaaggaa cgacgtacgc ccgaggaaga acaggaactg
    23641 cgtctaatca agaatgcttt agaggatttt tatattcctg aaggagaaac tttttcggag
    23701 agtaagacgg gtaaagaatt cagcgaaacg tcgaatgaag cgcaatcgtt gatgaaacat
    23761 ggaatcaatt ttgacaagtt agaagaacaa gattttacca aatatctttc ttatatgaca
    23821 cgtcactatg cgttagaaca aaagaaagta ctgacgcata gtgaacaaaa agaataccaa
    23881 cgcttgaacc aagcaataaa aaaatatcaa acagagccac acaaaaaagc ttcaggaaat
    23941 cttcctggag ttttttctat ggcttcagac gaagtgaaag gaagtgatct gaaggagatc
    24001 aagcaaacct tccaaagagg acaacaaaaa ggctcaatca tttatcaaga tgttgtctct
    24061 catgatcatt cgtatcttga aaaaatagga ttgtacgatc ccaaaacaga tactttagat
    24121 gaagaaggtt taaaagcagc tggaagaaaa atgatggaga cattgttcga gaaagagcaa
    24181 ctgaatgaaa caggctactg gatggcaacg attcaccgaa ataccaaaca tatccatatt
    24241 cattttgcag ttgttgaaaa aaaaaatacg cgtaaaccat acactgagat tgaaaatggt
    24301 gtgagttata tcgtaccgaa agggaagaga aggcaagcaa cattagacga aatgaagact
    24361 aattatgtac atgagttaga acgtttttct tatgaaaaaa atcgaatggg tatggagaaa
    24421 cgaaatttat tgacacggaa aagtgatttg cgtaacacct taaccaaagt agtgaaagag
    24481 ccagtacgtt atgatcgaca cgcaatgagc ttattaactg aagtatataa aaacttacct
    24541 aaaaagcgtt cggaatggca ttacggggat gaaaatcgaa ccaaactttc accatacaca
    24601 agagagaaac tcgatgagtt aacccaatat gtgttaacaa aagaacctgg atatcaagaa
    24661 tatatacaga tttcaaaagc attgaaagaa gaagcaaaaa gtctttatgg ggaaagcaag
    24721 agagaatcaa aggatgcaga aaaaaatgct ctctttgatt taaaaaagcg cacaggaaat
    24781 gccattctaa cagaactgaa gaaacaggat agtcaaataa aacctttaat agaaaggatg
    24841 gatcagatgc aatctattta caatgaacga tcctttcgaa cacttaaaca gttgccaacg
    24901 aatgaataca aatatagtta cgaagaattc atgaagcgca ggaaacaatt ccaacaaaga
    24961 gttgtcacgc aacggagttt gcgtcaatta aatcgaacga ttaatgccca tgaagaaaaa
    25021 taccacgcaa gtaaagcata cgaagagcaa caacgaagaa ttcaaatgga acaagaaaga
    25081 cagtaggagg aatagcaatg aaacaatatt tagatcaatg gaaagttata gaaggatcat
    25141 taagagaaga acgaatcgaa caacttcccg attgtttaga aaaagagcat ttgtttcaga
    25201 taagagaaat gttgataaac gaacagtttg atccaaatca atttttagtc gttgagtacc
    25261 cagctactgg tgtgtactgt tgtaatcatg tgaagggtga aaaatatttt atcattcaag
    25321 aatatgaggg caagttagcg ccttattata ctacttggga aatgaatgaa gaggggatca
    25381 ataacttccc gtgtaaaagc attgaagagt ctatttcact cacagaatgt tagttgttaa
    25441 gtgaataaaa gaagtaaaag agtcctgaac gtgattgtcc aggactcttt tttttcactg
    25501 aattgttttc cgccaattca ttgagtggtg cgatttccgt ctacattata tcaaaagaaa
    25561 ggaggaagaa gaaatggctt attcaacgat tggagttgtt gttgaaaaaa gcagagacaa
    25621 tcttgttttt gtgacagaaa ttcaaacagg cagagctttt gtagtcacag acaaagcggc
    25681 aaaagcatat caaagtggcg atattttaac gttaaatctg actacgaaga cgttcgtcga
    25741 tgcggcagaa gattatccat t
//
LOCUS       DAZKLD010000023        25543 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.023, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000023 DAZKLD010000000
VERSION     DAZKLD010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25543)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25543)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25543
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.023"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1304
                     /locus_tag="ACG8KR_000814"
     CDS             <1..1304
                     /locus_tag="ACG8KR_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0200210.1"
                     /translation="TIQLTDLNEEEKKQEISDWAILTRLLIQPTFIRSFTQQADPYDL
                     RKLQEKIMLASVRDESWLVVGNDENECSFRLEDNQLLIKNVLSISVFKEKQFLIRDFI
                     QKKMIAHGVFAYMRAYSEFIYHNTKQISQRLLFEKKDEIEHLPKMKQQNGEVVVDCNQ
                     FPGYDLFYQGLCFTSCWEMYYSRYYHQIIPKPIFLDIQQVEKVKELENEVICIQLYRD
                     PFNWQKPNNQMFQSYFRDQLGFDHLAWDNGVGLLKPPFVEYIYTDHTIQSVQYQNAQM
                     QPVPKKNASFFVTKSYDIQQGDYKERRVRGTLNAQAYFPWVDENRSRMMCYKVIDPTV
                     ALDNGIEAYCYYIREYLEVEVTDEKYQEYLLSLRIYVPSESLSELPLKEIKHRLSDVT
                     FKRIKKKRRSIQFDVKKGEQHLRVQFLDYRELEQLITLQKI"
     gene            1315..1524
                     /locus_tag="ACG8KR_000815"
     CDS             1315..1524
                     /locus_tag="ACG8KR_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0200211.1"
                     /translation="MDKQLYYRIMVPASLLSVCLFFVPNFPIRSVIGFLVMFGGMGLY
                     YYLLHKERKPASVYMKKTRSENKKR"
     gene            1682..2434
                     /locus_tag="ACG8KR_000816"
     CDS             1682..2434
                     /locus_tag="ACG8KR_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFQ0200212.1"
                     /translation="MLTEERRQKILDLLEQQKIVKSQDLVNLFNASESTIRRDLQELE
                     NSGFLERVHGGAKKEQLLNFEQNMSEKSLKNVHEKQQIAKSAAETINDEDVIYLDAGS
                     TTLEMIPFLQGKQITVVTNSVQHAANLVDLELATIILGGTIKLATNAVLGSNAIEQLK
                     NFHFNKAFMGMNGAHLEQGFTTPDPEEAAVKRLAMKNSEETFVLIDHTKFNQLSFTSV
                     APLEAATIITERCPLEFVNEFREKTTIKEAIQ"
     gene            2431..3345
                     /gene="pfkB"
                     /locus_tag="ACG8KR_000817"
     CDS             2431..3345
                     /gene="pfkB"
                     /locus_tag="ACG8KR_000817"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HFQ0200213.1"
                     /translation="MIYTVTLNPSIDYIVRVDGLKLGDLNRMKEDFKLPGGKGINVSR
                     ILKRIDSQSTALGFLGGFTGEFISKWLEKEQIQSLFTTVKEDTRINIKLKSDTETEIN
                     GLGPSISDEEIDELKQALGTVGQGDIVVLSGSAPASLRKGFYQELIDIIREKGADFVI
                     DTTGDDLKNALVKKPLLIKPNNHELAELYNTTFQSVEEIIPYGKKLLAEGAQNVLISM
                     AGDGALLFTEQGTYKTNVLVRPLKNSVGAGDSMIAGFIGAYSRTKDPIEAFKWGVACG
                     SATAFSDDLAARELIDELLPEVEITKID"
     gene            3363..5294
                     /locus_tag="ACG8KR_000818"
     CDS             3363..5294
                     /locus_tag="ACG8KR_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-specific PTS transporter subunit EIIC"
                     /protein_id="HFQ0200214.1"
                     /translation="MNIKDLMVKEAMIMDLQATDKKGAIDEMVQKMFDAGRISDIETY
                     KEGILAREAQTSTGLGDGIAMPHAKNSAVKEATVLFAKSQKGVDYEALDGQPTYLFFM
                     IAAPEGANDTHLQALAALSRLLIDADFVGKLKEANTPEEVQDLFQTAEEQKEAEEKAE
                     QETQAAEASAHPERKFVVAVTACPTGIAHTYMAEDALKKKAKEMGVEIKVETNGSEGI
                     KNRLTAEDIARAEGVIVAADKKVEMNRFDGKELINRPVSDGIRKPEELINLALSGTAP
                     VFHGDGKESSSEEGNADGTIGQRIYKDLMNGVSHMLPFVIGGGIAIALSFMIDQFIGV
                     PHDQLSNLGNYNQAASWFNQIGQAAFGFMLPVLAGFIASSIGDRPGLIVGFAAGALAN
                     TGGAGFLGALIGGFLAGYVIVFLRKLFKNLPKSLDGIKTILFYPVFGLLITGFLMLLV
                     NVPMKAINDALNGFLTSMSGSNAALLGALLAGMMAADLGGPINKAAYVFGTATLATTV
                     ATGGSVVMASVMAGGMVPPLAIFVATRLFKNKFSKTDQDAGLTNIVMGLSFVTEGSIP
                     FAAADPIRAIPSFIIGSALTGGLVGAFGIKLLAPHGGIFVVFLLSHPVMYLVFIAIGA
                     VVSGVIYGLLRKAPAAAEA"
     gene            complement(5405..6964)
                     /locus_tag="ACG8KR_000819"
     CDS             complement(5405..6964)
                     /locus_tag="ACG8KR_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HFQ0200215.1"
                     /translation="MEKKGKIKKLDKTQIYFILKGIFVGLLTGIVVSLFRLSIEKLSD
                     LVRSIYEMSREQPIYLIGLAICCILAAFFVGYLVKNEPDIKGSGIPQVEGQLRGELSM
                     NWFSVLWKKFIGGVLSVGAGLFLGREGPSIQLGASIGQGAGQLFRSPSSEEKILISSG
                     ASSGLAAAFNAPIAGLLFVLEEIHHSFSPLVWLTSFASAITANFVSLYFFGLRPVLYL
                     GDLPSLPLKYYGSLVILGVLLGILGFIYQKVLLALPKFYNRLPLPAYFHGFVPFLLIL
                     PIGYLWPSTLGGGNQIVLAFGHASLPIIMIIGLFVLRFVFSMISYGGGLPGGIFLPIL
                     TLGALIGSFYGNIMVDFGMDPIYIKDFVVIAMAGYFTAIGKAPLTAIILVTEMVGSLN
                     HLMPLGLAALVAYIVNDLLGGNPIYESLLERLLGNTQTASLKGARTTFDFPVTAESSL
                     DGLMVRDFNWPKNMLLISIRRGNQEILTHGDTIMNVGDILVILTDEANLPRIKQEIEA
                     KSSITNLKQSF"
     gene            7119..9353
                     /gene="pcrA"
                     /locus_tag="ACG8KR_000820"
     CDS             7119..9353
                     /gene="pcrA"
                     /locus_tag="ACG8KR_000820"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HFQ0200216.1"
                     /translation="MSSKAELLNGMNPRQKEAVLHTDGPLLLMAGAGSGKTRVLTHRI
                     AYLIEEKEVNPWNILAITFTNKAAKEMKERVNAILASGGEDVWVSTFHSMCVRILRRD
                     VDFIGYNRNFTIIDSSEQLTLMKRILKELNIDPKKYDPRSILGTISQAKNSLQTPQDF
                     AKMQGSYYEEIAAKCYAAYQKELQYNQCMDFDDLIMNTIRLFEEHPDSLTYYQNKFHY
                     IHVDEYQDTNHAQYTLVNLLAGRFRNLCVVGDADQSIYGWRGADMQNILDFEKDYPDA
                     AVILLEQNYRSTKNILSAANQVIENNSNRKPKNLWTENKEGNKITYYRADNERDETRF
                     IVDRMQEEIRSNHRNYGDFAILYRTNAQSRVMEETLLKANIPYKMVGGHKFYDRKEIK
                     DILAYLNVLANPQDSISFERIVNSPKRGIGPGSIEKLRSFASLHEWPLLEAAQNVDLA
                     NIGGKAGQQLGAFGEMIQEVTQMIPYLTVTELTKEVLDRSGYLEDLKIQNTLEAQARI
                     ENLEEFLTVTQEFDKQFEQQNEEDADAPEEKLTVFLNDLALVSDIDNLEEDASQVTLM
                     TLHAAKGLEFPVVFLIGLEEGVFPLSRALMEESELEEERRLAYVGITRAEEALYLTNA
                     FSRTLYGRTQYNRPSRFVEEIDQELLEIEGMRPTPKKTPVFAKKTAYSYKQPETAVVP
                     SKSATGGENNSWKPGDKVKHKKWGLGTVVRVSGTSKDLELDVAFPSQGVKRLLAAFAP
                     IEKA"
     gene            9370..11406
                     /gene="ligA"
                     /locus_tag="ACG8KR_000821"
     CDS             9370..11406
                     /gene="ligA"
                     /locus_tag="ACG8KR_000821"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HFQ0200217.1"
                     /translation="MEHEMTIDEAAKRAEELRTRLNQWSREYYVEDKPTVEDYVYDKE
                     YAELVAIEEQYPDLITSDSPTQRVGGKVLEGFEKVTHDIPLYSLNDVFSKEELIAFDQ
                     RVQKAVGRVVDYCCELKIDGLSVSLRYEDGNFVRGATRGDGTVGENITENLKTVRSVP
                     IKLKEPMNIEVRGECFMPKRSFVQLNQDREAEGKDIFANPRNAAAGSLRQLDSKITAK
                     RNLDTFLYTVADFGPMQAKTQYDALEELEKIGFHTNREKRLCHSIDEVWSYIEEYHDK
                     RVDLPYEIDGIVIKVNEFSLQDQLGFTVKAPRWAAAYKFPPEEVETLIENIEWTVGRT
                     GVVTPTAIMTPVRVAGTTVSRASLHNGDYIKLKDIRLKDTVLIYKAGDIIPEVSQVVL
                     DKRPKDSEEYQLPTHCPVCGSELVHLDEEVALRCINPKCPAQMKEGLNHFVSRNAMNI
                     DGLGPRVLEQMYDKKLVADVADLYKLTEEELLTLDKIKEKSANNILTAIDNSKDNSVE
                     RLIFGLGIRHVGAKAAKILAEHFGDLETLSKSDYESIIALDTIGDIIADSVVTYFSNE
                     EVHELMNELKQAGVNFEYKGLRNAQLQEVESPFKEKTVVLTGKLTRFTREEAKETIEN
                     LGGKVTGSVSKKTDIVVAGEDAGSKLTKAQELGIEVWTEDQMADALAKSRSVEE"
     gene            11512..11817
                     /gene="gatC"
                     /locus_tag="ACG8KR_000822"
     CDS             11512..11817
                     /gene="gatC"
                     /locus_tag="ACG8KR_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA];
                     GO_process: GO:0006450 - regulation of translational
                     fidelity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HFQ0200218.1"
                     /translation="MAISEEQVKHVAKLAKLAFADEELASFTDQLGKIIDMVEMLEEV
                     DTEGVPFTSNVVETVNVMRQDRAQAGWNRDELLKNVPETEDGFIKVPAIIDNGEAGA"
     gene            11817..13289
                     /gene="gatA"
                     /locus_tag="ACG8KR_000823"
     CDS             11817..13289
                     /gene="gatA"
                     /locus_tag="ACG8KR_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HFQ0200219.1"
                     /translation="MTELHNRSLEELHHMLVSKEITVQDLTKATFERIKETEPEVEAF
                     ITLNEEKALEQAKALDEKGIDESNVLAGIPIGIKDNIVTKDILTTAASKILYNFEPIY
                     DATVMDKIYQADMIPVGKLNMDEFAMGGSTETSYFKKTKNAWDPTKVPGGSSGGSAAA
                     VAAGQIPVSLGSDTGGSIRQPAAFNGIVGMKPTYGRVSRFGLIAFASSLDQIGPMTRT
                     VKDNALALTAISGYDEKDGTSSGVSVPDFAEGLTGDIKGMKIALPKEYLGEGVAPGVK
                     EAVLKAAETFRSLGAVVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRSEN
                     VKSLDDVYVNSRSEGFGEEVKRRIMLGTFSLSAGYYDAYFKKAGQVRTLIKRDFEKVF
                     ADYDLIIGPSSPTVAFGIGENINDPITMYMSDILTIPVNLAGLPGMSVPAGLSEGLPV
                     GLQIIGNYFDEKTMYQAAYAFEQATEFHKQQPAILGGKDE"
     gene            13286..14716
                     /gene="gatB"
                     /locus_tag="ACG8KR_000824"
     CDS             13286..14716
                     /gene="gatB"
                     /locus_tag="ACG8KR_000824"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HFQ0200220.1"
                     /translation="MNFETVIGLEVHVELKTNSKIFSPAPAHFGAEPNSNTNVIDWGY
                     PGVLPVMNKAALEFGMKAALALNCEISKDTHFDRKNYFYPDNPKAYQISQFDQPIGHD
                     GWIEIEVEGKKKKIRIERVHLEEDAGKNMHGIGGYSYVDLNRQGTPLIEIVSEADMRS
                     PEEAYAYLEALRSIIQFTEVSDVKMEEGSMRCDANISLRPYGQEEFGTKAELKNLNSL
                     SFVKKGLAFEEKRQAKVLLSGGEIQQETRRFDETTNKTVLMRVKEGSSDYRYFPEPDI
                     PKFVIDDEWIQKVKASLPEMPASRRERYIREFGLPEYDAMVLTLTKEMSDFFEAVLEN
                     GADAKQASNWLMGEVSAYLNSEKLELAETKLTPENLAGMIRLIADGTISSKIAKKVFR
                     ELITNGGEAQEVVEKNGWVQLSDPNKLLPIINEILDNNQQSVDDFKNGKDRAVGFLVG
                     QIMKATKGQANPGVVNKLLKDELAKR"
     gene            14726..15781
                     /locus_tag="ACG8KR_000825"
     CDS             14726..15781
                     /locus_tag="ACG8KR_000825"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFQ0200221.1"
                     /translation="MKKARVIYNPTSGKELLKKNLADILQALEEAGFEASAYATTPEP
                     DSAKNEACRVAELGFDLVVAAGGDGTINEVVNGIAPLEHRPKMAIIPAGTTNDYARAL
                     KIPRDNIKAAAEVIKKHQTVKMDIGLSDNSYFINIAAGGYLTELTYEVPSELKSIFGY
                     LAYLAKGAEMLPRVKPIKMRLKYDEGEYIGNASMFFLGLTNSVGGFEQIAPDAKLDDG
                     KFSLIIVKTANIFEILHIAALMLNGGRHVDDPRIIYTKTSSLFVETEDDINRPVMINL
                     DGEYGGEAPMHFQNLHQHIEFFANTDQIPDEAITGTDEEELEVVSKEFVKEVERLTDE
                     DIDGDGKIADETPNQKN"
     gene            15892..17265
                     /gene="rlmD"
                     /locus_tag="ACG8KR_000826"
     CDS             15892..17265
                     /gene="rlmD"
                     /locus_tag="ACG8KR_000826"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HFQ0200222.1"
                     /translation="MTEQQIVKKNERYTVEIVDLSYEGMGVAKIDGYPLFIENALPGE
                     EVEILVVKAGNKFGYGKVETFITQSPDRQPVENNALLRIGIAPLAHLKYEQQLVFKQK
                     QVEHVMAKIAKMPEVPVLPTVGMAEPFGYRNKAQIPVRRIDGQLATGFYKKNSHDLVP
                     IEDFYIQDPVIDQAIKVVRDILQRFQVRGYNEEKNEGQLRHIIIRRGHYSHEMMVVLV
                     TRKEKFFKSKEIAEAIHEELPEVVSVIQNINEEKTNVILGDKEKVLYGRDYIEDQLLG
                     KTYRISAKSFYQVNTQQAEYLYQTAIEFAELDKKDIVIDAYSGIGTIGLSLADRVDKV
                     YGMEVIPEAIEDAQFNALNNKIENVHYEVGKAEKVMKKWQEKGIKPSVIVVDPPRKGL
                     DHHFIESAIEMSPERIVYISCNPATFARDAKLFAEAGYAVKKVQPVDLFPQTHHIELV
                     ASFVKGE"
     gene            17268..17396
                     /locus_tag="ACG8KR_000827"
     CDS             17268..17396
                     /locus_tag="ACG8KR_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HFQ0200223.1"
                     /translation="MDQKEKMKELIKKKQGGGRSKQMETPKNDRKNMRKGPKIFNK"
     gene            17772..17960
                     /locus_tag="ACG8KR_000828"
     CDS             17772..17960
                     /locus_tag="ACG8KR_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HFQ0200224.1"
                     /translation="MEEHQQVIDRIYEATRYLPLDRLCLSPQCGFASTEEENVLFEEQ
                     WKKFLLSKALQKKFGENH"
     gene            18038..19051
                     /locus_tag="ACG8KR_000829"
     CDS             18038..19051
                     /locus_tag="ACG8KR_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFQ0200225.1"
                     /translation="MKKKILKFIGWFFGIILGIAFAIFLAFQLSPRPGAFLINHMFSN
                     TVQITDKKTFDQAEKNVAKKTDLTYESKYKENTYDIYYPKNSKGPVPVLFWVHGGGFV
                     GGDKSGVKEFATKLVSDANIAVVAMNYELAHDSEYPNQVLQVNELIKDLLAEEKDDKL
                     LDMEQLFFGGDSAGSQIALQYAAIQTNDAYAKQMKIKQLLPKDSLKGTISYCGPVDLK
                     LTAQQHSDDRFMKFFVKTVAWSLIGTKDWKTSEQLKEASVADHVSKEFPPTYITDGNA
                     YSFQEQGQALENRLTELKVPVQSLFYTDTKKEITHEYQFDYTLKESQNCYQQTLDFVN
                     KYK"
     gene            complement(19118..20152)
                     /locus_tag="ACG8KR_000830"
     CDS             complement(19118..20152)
                     /locus_tag="ACG8KR_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0200226.1"
                     /translation="MVGIRDVARKANVSPATVSRVLNNDQTIHVAQTTRQRIVEAAAA
                     LDYDISTRKYIKKRMPSIGVISTIGKASEEQDIYYKHLRVGMEEEARRLHLGMNRIYN
                     LSDNPKQWEDLDQLGAVIVVGTVTKESITALLKQNMQIVVVDNPDIQQEVDMVYADLE
                     RMTKTVLELFLENGHKRIAYIGGYKVDMDEFGGKQISINEKRMRAYQHFMIDHHLEKY
                     MSYHLGGWTEKQGEVLATNLLENESPLPTAILVGSDPMAIGVYRALKQKNIQIGEEIV
                     VASFDNIETIANLSPGLTSVQINAKSIGQAAVRMALERIDGIRDEPIILTYPARLIVR
                     ESFIPRTKRY"
     gene            20267..22492
                     /locus_tag="ACG8KR_000831"
     CDS             20267..22492
                     /locus_tag="ACG8KR_000831"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HFQ0200227.1"
                     /translation="MDITHRKTDKNFILFDEKSRVFHLRNTFLSYLIKIEESNVLAHI
                     YFGKPVKQYKDNKNYPRRDRGFSGNVPLNPDRSLSKDTLPQEYSSHGSMDFRTPASII
                     QRKNGSDLLDLRYDSHYITDGKPEIEGLPQTYVMDKSEAQTLVISLKDRETAIYFDLF
                     YTIFTDRAVITRSVKIRNETGETIKLEKAASFQLDFAHTRRFDEVIALPGAHVNERQI
                     SRQSVLSGTKVFESRRGTSSHHMNNFIALVHHHTTENTGEAIGLQFVYSGNHSFELEK
                     DQINQLRVVGGINSHRFSWELNAGQSFQTPEMILSYSSQGLNKMSQIHHELLRERIAR
                     GRHQFAERPILVNNWEATYFDFNSEKIKAIIDEAKELGIEMFVLDDGWFGKRDADNSS
                     LGDWFEYEGKLTNGLREIADYAHSKGLKFGLWFEPEMISVDSELYRTHPDFLMQEPGR
                     MPSASRSQHVLDFTRLDVRQTIEKQMRKILDTIPLDYIKWDMNRSLSDVYSITLDPQR
                     QGEVAHRYMLGLYELLEHLTTDYPEILWEGCSGGGGRFDAGFIYYMPQSWTSDNTDAV
                     ERMKIQYGTSLAYPISSITAHVSAVPNHQTDRSTSLKTRGETAMSAVFGYELDLTKLS
                     PEEKKQVKDQIISYQTIRPVIQYGHYYRLASPFEENIAAWMFVSPKQDEAIVFLGRIL
                     ASAQPAFHEVYLMGLDDEALYQEQTSKRIFSGAELMTVGLYFPDFQGDFQTELLHFKK
                     L"
     gene            22510..24393
                     /locus_tag="ACG8KR_000832"
     CDS             22510..24393
                     /locus_tag="ACG8KR_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HFQ0200228.1"
                     /translation="MKANIKWLDDPQVFRVNQSNAHSDHVCYATREEVETTSSLKQSL
                     NGSWRFCYSPNPSVRPKTFYKKDFDACSFDEITVPSHIELANYDKIHYINTMYPWEGH
                     VFRRPPFTTSPEKEAEGIFSAAKDNPVGSYLTTFRLEEGLKGKQISIHFAGVEQAMFI
                     WLNGHFIGYAEDSFTPSEFDLTPYLEEENILAVEVYKRSTAAFLEDQDFFRFFGIFRD
                     VFLVARGQNHLEDLWIKPRLDDKCQNGKLEVDLSFEQVAKNSQLNVAILDPDGKEIYQ
                     QQYPLKEQMTIRIDGFENVALWGHFQPNLYKLELETIDEEKRVTEYIDYSFGFRKIEI
                     KDKIIYFNGKRMIINGVNRHEWHPKKGRAITIEDMEKDLAIIKNAKINGVRTSHYPNQ
                     ITWYYLCDQAGIHVMAETNLESHGSWQKMGAFEPSYNVPGAVPQWQAAVLDRAKTNFE
                     TLKNHPSILFWSLGNESYAEENIRQMNDYFKKVDPDRLVHYEGNFPNPAFEDSISDVK
                     SCMYAPPEEIIDYLENKAEKPFILCEYMHDMGNSLGGMDSYMTLLDRYEMYQGGYIWD
                     FKDQAIQVTDPITGREVMRYGGDFDDKPSDYEFSGNGIVFADGQEKPAMQEVRYYYEK
                     YSK"
     gene            24374..25348
                     /locus_tag="ACG8KR_000833"
     CDS             24374..25348
                     /locus_tag="ACG8KR_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HFQ0200229.1"
                     /translation="MKNTVNKSHMDTEKVAIVFGDCTLGVKSGNTHYIFSYTRGGLES
                     LNKNGKEWLYRETLPVFWRATTDNDRGSGFGFKSGMWLGAGLYPKVKEIEVLVDNKKI
                     DLPIAPVNNQYSSNEYAETVEIIFTLEINTVPKTQVTISYQIAGEGKMLVNMHYYGQK
                     GLPELPALGLRFIMPTKAKFFEYEGLSGETYPDRMAGGVEGIYKVAGLPVSPYLVPQE
                     CGMHMETKWVELTRNQTLDNTDKDQEDFSLRFEQQDTPFAFSALPFKAEELEQATHIE
                     ELPPERRTVLTIFAKVRGVGGINSWGADVESPYRISSEEDHEIGFIIQ"
BASE COUNT         8568 a         4643 c         5479 g         6853 t
ORIGIN      
        1 tgactatcca gctcacagac ttaaatgaag aagaaaaaaa acaggaaata agcgattggg
       61 ctattttgac acgtctgttg atccagccaa catttatccg ctctttcaca caacaagcgg
      121 atccgtatga tcttcgaaag cttcaagaaa aaataatgct agcatcggta agagacgaaa
      181 gttggttagt tgtaggaaat gacgagaacg aatgtagttt tcgcttggaa gataatcaat
      241 tattgataaa aaatgtattg tctatctctg ttttcaaaga aaagcaattt ttgattcgag
      301 acttcattca gaaaaaaatg attgctcatg gggtgttcgc ctatatgcga gcttatagcg
      361 agtttatcta tcacaataca aaacagatct ctcaacggct attgtttgag aaaaaggatg
      421 aaatcgaaca tttacctaaa atgaaacaac aaaacgggga agtagtagtc gattgcaacc
      481 aatttccagg atacgatctt ttttaccaag gtttatgttt cacttcgtgt tgggaaatgt
      541 attatagtcg ctattatcat caaatcatcc ctaaaccgat ttttctcgac atccaacaag
      601 tagaaaaagt gaaggaatta gaaaacgaag tcatatgtat ccagctttat cgagacccct
      661 ttaactggca aaagccaaac aatcaaatgt ttcaatccta ttttagggac cagcttgggt
      721 ttgatcatct tgcttgggac aatggtgtcg gtttattgaa accgccattc gttgaataca
      781 tttacactga ccatacgatc caatctgtcc agtatcagaa tgcacaaatg caacctgttc
      841 ctaaaaagaa tgcttcattt tttgtaacaa aaagttatga tattcaacaa ggagattata
      901 aagaaaggcg tgttcgcgga acactaaatg ctcaggcgta ttttccttgg gttgacgaga
      961 atcgctcgcg catgatgtgc tataaagtta tcgatccgac tgttgctttg gataatggaa
     1021 tcgaagctta ttgttattat attagggaat atctagaagt agaggtaaca gatgaaaaat
     1081 atcaagagta tcttttatcc ttaagaatct atgttccttc agaatccctg tcagaattac
     1141 cgttaaagga aatcaaacat agactctctg atgttacctt caaacgaata aagaaaaagc
     1201 ggcgaagtat ccaattcgat gtcaagaaag gtgagcagca tctgcgtgtg caatttttag
     1261 attaccgaga attagaacaa ttgattactt tacaaaaaat atgaggtgaa gaaaatggat
     1321 aaacaactct attatcgaat aatggttccg gcatcattat tatccgtctg tctctttttt
     1381 gttcctaatt ttcctattcg atcagtcatc ggttttttgg tcatgtttgg cgggatggga
     1441 ctttattatt atcttcttca taaggaaaga aaacctgctt ctgtttatat gaaaaaaaca
     1501 agaagcgaaa ataaaaaacg ataaaacggc ttgatgatag acaaaaaaat aaaaaaccag
     1561 tctttttgac tggtttttta ttttttttga ctgatttata atttttatga acgtttttga
     1621 ctgatttata ttgcgagtag aaaaaaaata tgctattatt tgtattgagg tgatcgtgaa
     1681 aatgcttaca gaggaacgta gacaaaaaat cctagacttg cttgaacagc agaaaatcgt
     1741 gaaatcacaa gatcttgtaa acctttttaa tgcttctgaa tcaacgataa gacgtgattt
     1801 acaagagctg gagaatagtg ggtttctaga aagagttcat ggcggagcta agaaagagca
     1861 attattgaat tttgagcaga atatgagtga aaaatcactc aaaaacgttc acgaaaaaca
     1921 gcaaatcgcc aaatcggcag ctgaaaccat taatgacgaa gatgtcattt atttagacgc
     1981 tggctctact acgttagaaa tgattccttt tctgcaagga aaacagatca cagtagtgac
     2041 aaattccgtt caacatgcgg caaatttagt tgatttggag cttgctacca ttattttagg
     2101 tggaacgata aagctggcaa ctaatgctgt tttaggatct aatgcgatag aacaactaaa
     2161 aaattttcat tttaataaag catttatggg aatgaacggt gcccatcttg aacaaggatt
     2221 cacgactccg gacccagaag aggcagctgt taaacgttta gccatgaaaa attccgaaga
     2281 gacatttgta ttgattgatc ataccaaatt caaccagttg tccttcacct ctgttgcacc
     2341 tttggaagca gccacgatca taaccgagcg ttgtccatta gaattcgtta atgaattccg
     2401 tgaaaagaca acaattaagg aggcaataca atgatttaca cagtaacact taatccatca
     2461 attgattata tcgtacgcgt agatgggtta aaactaggcg atttgaaccg aatgaaggaa
     2521 gacttcaagt tacctggagg aaaagggatc aatgtttccc gtattttgaa acggatcgat
     2581 tctcaatcta cagcactagg gtttttagga gggttcactg gcgaatttat ttcgaagtgg
     2641 cttgaaaaag aacaaatcca gtctttgttc acaaccgtaa aagaagatac aagaatcaat
     2701 atcaaactaa aatcagatac tgaaactgaa attaacggtt tgggcccatc tatttcagat
     2761 gaagaaatcg atgagttgaa gcaagcatta ggaacagtag gacaaggaga tatcgtcgta
     2821 ttgtcaggaa gcgcgccggc tagcctgcga aaaggatttt atcaagaact gattgatatc
     2881 attcgagaaa aaggtgcaga tttcgtgatt gatacaacag gtgatgacct aaaaaatgca
     2941 cttgttaaga aaccattgtt gatcaaacca aacaatcacg aattagctga gctttacaat
     3001 acaaccttcc aatctgtaga agaaatcatt ccgtatggta aaaaattgct agctgaaggg
     3061 gcacagaatg tgctgatctc aatggctgga gacggtgctt tgctcttcac ggaacaagga
     3121 acttataaaa cgaatgtatt agttcgccca ttgaaaaatt cagtaggtgc aggggattca
     3181 atgattgccg gctttattgg ggcttacagc cgtacgaaag atccaatcga agcctttaaa
     3241 tggggagttg cttgcggaag cgcaacagct ttttctgatg acttggcagc tcgcgaattg
     3301 attgatgagt tgctgccaga agttgaaatc acaaaaattg attagtttca aggaggataa
     3361 tcatgaatat caaggattta atggtcaaag aagcgatgat catggatttg caagcgactg
     3421 ataaaaaagg cgcaatcgat gaaatggtcc aaaaaatgtt tgatgccggc cggatttccg
     3481 atatcgaaac atataaagaa ggaattctag cccgcgaagc acaaacttct acaggtcttg
     3541 gtgatggaat cgccatgcct cacgcgaaaa acagtgcagt taaagaagca acagttttat
     3601 tcgcaaaaag ccaaaaagga gtagattatg aagccttaga cggacagcca acttatctgt
     3661 ttttcatgat cgcagctcca gaaggcgcaa acgacacaca tttgcaagca ttagcagcac
     3721 tatctcgatt gctgattgat gcagatttcg taggaaaatt aaaagaagca aatacaccag
     3781 aagaagtcca agacttgttc caaacagctg aagagcaaaa agaagcagaa gaaaaagcag
     3841 aacaagaaac acaagctgca gaagcttctg ctcatccaga aagaaaattc gttgttgccg
     3901 ttacagcttg tccaacaggg atcgctcaca cttacatggc agaagatgct ctgaagaaaa
     3961 aagcgaaaga aatgggtgtg gagatcaaag tcgaaacgaa cggttcagaa ggaatcaaaa
     4021 accgattgac tgctgaagac atcgcccgtg cagaaggtgt aatcgttgca gcagacaaaa
     4081 aagtagagat gaatcgcttt gacggaaaag aactgatcaa tcgtcctgtc agtgatggta
     4141 tccgtaaacc agaagagctg atcaatctag cccttagtgg tactgcacca gtattccatg
     4201 gtgatggaaa agaaagcagt tcagaagaag gaaatgcgga tggtactatc ggacaaagaa
     4261 tttacaaaga tttgatgaac ggcgtatcac acatgttacc tttcgttatt ggtggaggaa
     4321 tcgctattgc gctttctttc atgatcgacc aatttattgg cgtgccacat gatcaattat
     4381 caaatctagg gaattacaat caagctgcta gctggttcaa ccaaatcgga caagctgcat
     4441 ttggtttcat gctgccggta ttagcaggat ttattgcttc aagtatcggt gaccgccctg
     4501 gtttgatcgt tggatttgct gctggtgcat tagctaatac aggcggtgca ggattcttag
     4561 gtgcactgat tggtggattc ttagctggtt atgtcatcgt atttttacgt aaattattca
     4621 agaacttacc aaaatcattg gatggaatta aaacgatcct cttttatcca gtgtttggct
     4681 tactgattac tggtttcttg atgttgctgg tcaatgtccc tatgaaagca atcaatgatg
     4741 cattgaacgg tttcttgaca agtatgagtg gttcgaatgc tgctttactt ggtgcattat
     4801 tagctggaat gatggcggca gatttaggcg gtccaatcaa caaagccgct tatgtgtttg
     4861 gtacagcaac tttagctacg actgttgcaa ctggcggaag tgtcgtgatg gcttctgtta
     4921 tggctggagg aatggtacct ccattggcaa ttttcgttgc tacacgtttg tttaagaata
     4981 aattcagcaa aacagatcaa gatgctggtt tgacgaacat cgtgatggga ctatcgtttg
     5041 tcacggaagg atcgattcca tttgcagcag ctgacccgat tcgtgccatt ccaagtttta
     5101 ttattggttc tgctttaact ggcggattag tcggagcttt cgggatcaaa ttactggctc
     5161 ctcatggcgg gattttcgtt gtattcttac tgagtcaccc ggtcatgtat ttggtgttta
     5221 ttgctatcgg agcagtcgta tctggtgtga tttatggttt attgagaaaa gctcctgcag
     5281 cagcagaagc atagaaataa aaaagaagag gctggttttt cccagtctct tcttttttta
     5341 tagaatcata tagaatcaag tagtgtagat gaatagttgt tgtaaaatag tttttccatg
     5401 ttcgttaaaa tgattgcttc aaatttgtga tagaagattt agcctcgatt tcctgcttga
     5461 ttcttggcaa gtttgcttca tcagtcaaga taaccaaaat atctcctaca ttcatgatcg
     5521 tatcaccgtg agtcaagatt tcctgatttc ctctgcgtat agagattagc agcatatttt
     5581 taggccaatt gaagtctcgt accattaatc cgtctaaaga actctctgcc gttactggaa
     5641 aatcaaaagt tgtccttgca ccttttagtg aggcggtctg agtatttcca agtagacgtt
     5701 ccaataatga ttcgtaaatt ggattaccgc cgagaaggtc attgacgata taagcaacaa
     5761 gagcagccaa tcctaaaggc atcagatgat taagagagcc aaccatttct gtcactaaaa
     5821 taattgctgt cagcggtgct tttccaatcg ctgtgaaata accagccatg gcaataacga
     5881 caaaatcctt gatgtagatt ggatccatcc cgaaatcaac cataatgttt ccataaaagc
     5941 taccgattaa agcccccaat gtcaatatcg gaaggaaaat cccccctgga agaccaccac
     6001 cgtaagaaat catggaaaaa acgaatcgaa gaacaaacaa accgataatc ataatgattg
     6061 gcaaggaagc atgtccaaaa gctaaaacga tttgattgcc tccgcctaga gtactaggcc
     6121 ataaataacc aattggcaaa atcagcaaga aaggaacgaa cccgtgaaaa taagctggta
     6181 aaggcaatcg gttgtaaaat tttggcaaag ccagcaagac cttctgataa ataaaaccta
     6241 agatgcctag aagtactcct aagatcacta aggaaccata atatttcaac ggcaaagaag
     6301 gaaggtcacc tagatataaa acaggtctca agccgaaaaa gtaaagagag acgaagttag
     6361 cagtgattgc tgaagcaaat gatgtcagcc aaacaagtgg agaaaaggag tgatggattt
     6421 cttctaaaac aaagagtaat cctgcgatag gagcattgaa tgctgctgca agtccgctac
     6481 ttgcaccgct agaaattaaa atcttttctt ctgaagatgg actccgaaac aattgtccag
     6541 ctccttgtcc aatggatgcg ccaagctgta tagaaggtcc ttctctacct agaaaaaggc
     6601 ccgcaccaac tgaaagtaca ccgccaatga actttttcca taaaacagag aaccagttca
     6661 ttgataattc cccgcgaagc tgtccctcta cttgtggaat cccgcttccc ttgatatccg
     6721 gttcattttt gaccagataa cccacgaaaa atgctgcaag gatgcagcaa atagctaggc
     6781 caattaagta aatcggctgt tcccgactca tttcatagat acttcgaact aaatcgctta
     6841 gcttttcgat agataatcga aacaggctaa cgacgattcc tgtcagcagt ccaacaaata
     6901 ttccttttag aataaaatat atctgagtct tatctaattt cttgattttt cctttttttt
     6961 ccattcaaac gcctcgcttt cttttaattt agcgcgaaat caacgaattg acaaggaaaa
     7021 atccataaaa agaaacaaat gattttgaac tagctagttt aatgaaagcc aagaaaatgc
     7081 tataattaac aagattgata aaagaaagga tgttgttaat gtcatccaaa gctgaattat
     7141 taaacgggat gaacccccgt cagaaagaag ctgttcttca cactgacgga ccattactgc
     7201 taatggcagg tgcaggaagt ggaaagacga gagtgctgac acaccgcatt gcctatttga
     7261 ttgaggaaaa agaagtcaat ccttggaaca tcctagcgat tacttttacc aataaggctg
     7321 caaaagaaat gaaagaacgt gtcaatgcga ttctcgcatc tggaggcgaa gatgtctggg
     7381 tatcgacttt tcactcgatg tgtgtgcgta ttttaagaag agatgttgat tttatcgggt
     7441 ataaccggaa ctttacaatc atcgattctt ctgaacagct gaccttgatg aaacggatac
     7501 taaaagaact gaacattgat ccgaaaaaat atgatcctcg aagtatatta ggaacaatct
     7561 ctcaagcaaa aaacagcctg cagacccctc aggattttgc aaaaatgcaa ggcagttatt
     7621 acgaagagat tgcggcaaaa tgttatgctg cttaccaaaa agagcttcaa tataatcaat
     7681 gtatggactt tgacgacctg atcatgaata cgattcgatt atttgaagaa catcctgatt
     7741 cactcacata ttatcaaaac aaattccatt atattcatgt agatgagtac caagatacaa
     7801 atcacgcaca gtatacgcta gtcaatttat tggcaggtag atttaggaac ctgtgcgtcg
     7861 tgggagatgc agatcaaagt atttacggct ggcgcggtgc agatatgcag aacatcttgg
     7921 attttgaaaa agattatcca gatgctgcag taattttgct agaacaaaac tatcgctcca
     7981 cgaaaaacat ccttagcgca gcaaaccaag tcattgaaaa caatagcaat cgaaaaccaa
     8041 aaaacttgtg gacagaaaac aaagaaggaa acaagatcac ttactaccgt gcagataatg
     8101 aacgagacga aacccgtttt atcgtagatc ggatgcaaga agagattcga agcaatcatc
     8161 ggaattacgg cgactttgcc atcttgtacc ggacgaacgc acaatctcgt gtaatggaag
     8221 aaactctgtt gaaagcaaat attccttata aaatggttgg agggcacaag ttctacgatc
     8281 gtaaagaaat caaagatatt ttagcgtatt tgaatgtgct ggcaaatcct caagattcaa
     8341 tcagttttga gcgtatcgtt aattcgccca aacgaggcat cggaccagga tctatcgaaa
     8401 aactacgcag ttttgcttct cttcatgaat ggccactgct tgaagcagcg caaaatgtgg
     8461 atctggcaaa tatcggtgga aaagcaggac agcaattagg tgcatttgga gagatgatcc
     8521 aagaagtcac tcagatgatc ccatacttga cagtgacaga attgacgaaa gaggttttgg
     8581 atcgtagcgg atatctagaa gacttaaaaa tccaaaatac attagaagca caagcacgca
     8641 tcgaaaactt ggaggaattt ttaacggtca cacaagaatt cgacaaacaa ttcgaacagc
     8701 aaaatgaaga agacgctgat gcaccagaag aaaaactaac ggtcttctta aatgatctgg
     8761 cacttgtttc cgatattgac aatttagaag aagatgcttc ccaagtgaca ttgatgactt
     8821 tgcatgcagc caaaggatta gaattcccag ttgtattcct gattggtcta gaagaggggg
     8881 tttttccttt atctcgagca ttgatggaag aaagcgaact ggaagaagaa cgccgtctcg
     8941 catatgtggg gatcacgcga gcagaagaag cgttgtattt aacaaatgct ttctccagaa
     9001 cattatatgg acggacgcaa tataaccgac cttctcgttt tgtagaagaa atcgatcagg
     9061 agttactgga aatcgaaggc atgcgtccta cgcctaaaaa aacacctgta ttcgctaaaa
     9121 aaacagcata tagctataaa cagccagaga cagcggttgt tccctctaaa agtgctactg
     9181 gaggggaaaa taacagctgg aaacctggag ataaagtcaa acacaaaaaa tggggtctag
     9241 gaacagtcgt ccgagtaagc gggacgtcga aggatctaga actagatgtg gctttcccaa
     9301 gtcaaggagt aaaacgtctt ttggctgcct ttgcgccaat tgaaaaagct tagtagtgga
     9361 gggtaaatga tggaacatga aatgacgatt gacgaagctg ctaaacgagc agaagaactt
     9421 cgtacaagac tgaaccaatg gtctcgcgaa tactacgtag aagataaacc aacagtggaa
     9481 gactatgttt atgataaaga atacgcagaa ttagtagcaa ttgaggaaca atatcctgat
     9541 ttgatcacgt ctgattctcc cactcaacga gtaggcggaa aagtattaga aggttttgaa
     9601 aaagtcactc atgatattcc gctttacagt ttgaacgacg ttttcagcaa agaagagctg
     9661 atagcttttg atcaacgtgt acaaaaagca gtgggacgtg tagttgacta ttgctgtgaa
     9721 ctaaaaatag atggactttc cgtttctttg cgttatgaag atggaaattt cgtgagaggt
     9781 gcgactcgcg gggatggaac tgtaggagaa aacatcactg aaaacctaaa aactgtccgg
     9841 tcggtcccaa tcaaattaaa agaaccaatg aacatcgaag taagaggcga atgtttcatg
     9901 ccaaaacggt catttgtcca attgaaccaa gacagagaag cagagggaaa agatatcttt
     9961 gcgaatcctc ggaacgccgc agctggttct ttgcgtcagt tagattctaa aatcacagcg
    10021 aaaagaaatc tggacacttt tttatacaca gtagctgatt tcggtcctat gcaagcaaaa
    10081 acccagtatg atgcgctaga agagctagaa aaaattggat tccacacgaa tcgggaaaaa
    10141 cgtctatgtc attcgattga tgaagtgtgg tcatacattg aagaatatca tgacaaacgt
    10201 gtggatttgc cctatgaaat cgatggaatc gtcatcaaag tgaatgaatt ctcgctacaa
    10261 gatcaacttg gttttactgt caaagcaccg agatgggcag cagcttacaa gtttccgcca
    10321 gaagaagttg aaacgctaat cgaaaacatc gaatggacag tcggtcgaac aggggtagta
    10381 acgccaacag ctatcatgac accagtccga gtagcaggaa cgacagtaag ccgtgccagc
    10441 ctgcataacg gtgattatat taagttgaag gatatccgct tgaaagatac agttctgatt
    10501 tataaagcgg gagacatcat cccagaagtt tcgcaagtcg tgttagataa acgacctaaa
    10561 gacagcgaag aataccaatt gcctacacat tgtccagttt gtggaagtga acttgtccat
    10621 ttggatgaag aagtagcctt acggtgcatc aatcctaaat gccctgctca aatgaaagaa
    10681 ggtttgaacc actttgtttc aagaaatgcg atgaatatcg atgggttggg gccgcgcgta
    10741 ctagaacaga tgtatgataa aaaactagta gcagatgttg ctgatcttta caaattgaca
    10801 gaagaagagt tattgacgtt ggacaaaata aaagaaaaat cagcaaataa tattttgaca
    10861 gcaatcgaca acagcaaaga taattcggtc gagcggttga ttttcgggtt aggaatccga
    10921 catgtcggag cgaaagcagc aaaaatttta gcagaacatt tcggtgatct agaaacactg
    10981 agtaaaagtg actatgagtc gattattgcg cttgatacga tcggtgatat cattgccgac
    11041 agtgtagtca cttatttcag taacgaagaa gtccatgaat tgatgaatga attaaaacaa
    11101 gcaggcgtca atttcgaata caaagggctt cgaaatgccc aattgcaaga agtcgagtct
    11161 ccttttaaag aaaaaacagt agtccttact ggtaaactaa cacgatttac tcgtgaagaa
    11221 gcaaaagaga cgatcgaaaa tcttggcggg aaagtgactg gtagcgtatc gaaaaaaacg
    11281 gatatcgtgg tagcaggaga agatgctggc agcaagctga ccaaagcaca agaattagga
    11341 atcgaagttt ggacagaaga tcaaatggca gatgcattgg ctaaaagtcg ctcagtagaa
    11401 gaatgaaaaa tgagtttttt cagacaaatt tcaataaaca atttcagaca cggcactctt
    11461 gtgttaaaat agagagcaaa gtttttaact cgtttagaaa gaaggggtag tatggcgatt
    11521 agtgaagaac aagtgaaaca cgttgccaaa ttagcaaaac ttgcgttcgc agatgaagaa
    11581 cttgcaagtt ttaccgacca attaggtaaa atcatcgata tggttgaaat gctggaagaa
    11641 gtagatacag aaggtgttcc atttacgtcg aatgtggttg aaacagtaaa cgtgatgcga
    11701 caagatcggg cgcaagctgg atggaaccgc gatgaattat tgaaaaatgt gccggaaaca
    11761 gaagacggct ttatcaaagt acccgcaatc atcgataatg gggaggctgg cgcataatga
    11821 cagaattaca caatcgttct ctagaagaac tgcatcatat gcttgtatcg aaagaaatca
    11881 ctgtacaaga tttgacaaaa gcaacatttg aacgaatcaa agaaacagag cctgaggtcg
    11941 aagcattcat tacattgaac gaagaaaaag cattggaaca ggcaaaagct ttagatgaaa
    12001 aagggattga tgaaagcaac gtccttgctg gtattccaat tggtatcaag gataatatcg
    12061 taacaaaaga tatcttgaca accgctgcat ctaagatcct ttacaatttt gaaccaattt
    12121 atgatgcaac ggtcatggat aaaatctatc aagcagatat gatccctgtt ggtaagctaa
    12181 acatggatga atttgctatg ggtggaagta cagaaacttc ttatttcaag aaaacaaaaa
    12241 atgcttggga cccaacaaaa gtacctggtg gctcttctgg tggttctgca gcagcagttg
    12301 cagctggaca aattccagtt tctttaggta gtgatactgg gggaagtatc cgccaaccag
    12361 cagcttttaa cggcatcgta gggatgaaac ctacttatgg ccgtgtctca cgttttggtt
    12421 tgattgcctt tgcttcatct cttgaccaaa tcggaccaat gacccgtacg gtgaaagata
    12481 atgcattagc attgacagct atcagcggtt atgacgaaaa agacggtact tcttcaggcg
    12541 tttcagttcc tgattttgca gagggactga caggcgacat caaaggaatg aagattgctt
    12601 tacctaaaga atacttaggc gaaggtgtgg cccctggcgt aaaagaagct gtattgaaag
    12661 cagcagaaac attccgttca ttaggtgctg ttgtggaaga agtcagcttg cctcattcaa
    12721 aatacggtgt ggctgtttat tatatcattg cctcatcaga agcaagttcc aacttgcaac
    12781 gattcgatgg tatccgctac ggttatcgct cagaaaatgt gaaatcatta gatgatgtat
    12841 atgtcaactc acgttcagaa ggattcggtg aagaagtaaa acgccgtatc atgttaggta
    12901 cattctcctt aagtgctggt tattatgacg cttacttcaa aaaagcggga caagtccgta
    12961 cattgatcaa acgagatttt gaaaaagtct ttgcagatta tgacttgatc atcgggccat
    13021 cttcaccaac agttgcattc ggaatcggtg agaacattaa cgatccaatc acaatgtaca
    13081 tgagtgacat cttaacgatc cctgtcaact tggctggact tccaggtatg tctgtaccgg
    13141 caggtttatc agaaggatta ccagttggat tgcagatcat cggtaattat tttgatgaaa
    13201 aaacgatgta tcaagcggca tatgcgtttg aacaagcaac agaattccat aaacaacaac
    13261 cagctatttt aggagggaaa gacgaatgaa ttttgaaaca gtcattggac tagaagtcca
    13321 cgtcgagtta aaaaccaact caaaaatttt ctctccagcg ccagctcatt ttggtgcaga
    13381 accaaacagc aatacaaacg tgatcgactg gggttatcca ggcgtgctgc cagtaatgaa
    13441 taaagctgcg ctggaattcg ggatgaaagc agctttggca ttaaactgtg aaatctctaa
    13501 agatactcat tttgaccgga aaaattactt ttacccagat aatccaaaag catatcaaat
    13561 ttcccaattt gatcaaccaa tcggtcatga cggatggatc gaaatcgaag tagaaggtaa
    13621 aaagaaaaaa atccggatcg aacgtgtcca tttagaagaa gatgctggaa aaaacatgca
    13681 tggtattggc ggttattctt acgttgattt gaaccgacaa ggcacgcctt tgatcgaaat
    13741 cgtttctgaa gctgatatgc gttcaccaga agaagcatac gcttatctag aagcattgcg
    13801 atcaattatc caatttacag aagtcagcga tgtgaaaatg gaagaaggct ctatgcgctg
    13861 tgatgccaat atttctcttc gcccttatgg acaagaagag ttcggtacga aagcagaatt
    13921 gaaaaacttg aactctttat ctttcgtgaa aaaaggttta gcttttgaag aaaaacgtca
    13981 agcaaaagtt cttctttcag gaggagaaat ccaacaagaa acacgccgtt ttgacgaaac
    14041 aacgaataaa acagttttga tgcgtgtcaa agaagggtct agtgattatc gttacttccc
    14101 agagcctgat atcccgaaat tcgtgatcga tgacgaatgg attcaaaaag taaaagctag
    14161 tttgcctgag atgccagctt ctcgtcgcga acgctatatc cgtgagtttg gacttccaga
    14221 gtatgacgca atggtattga cacttacaaa agaaatgtct gatttttttg aagctgtttt
    14281 ggaaaatggt gcagatgcca agcaagcatc taactggttg atgggtgaag tttctgctta
    14341 cttgaacagt gagaaactgg aattagctga gacaaaattg acacctgaga acttagcagg
    14401 aatgattcgt ttgatcgccg atggaacgat cagctcaaaa atcgccaaaa aagtcttccg
    14461 tgaattgatc acaaatggcg gagaagccca agaagtagtt gaaaaaaatg gttgggtgca
    14521 attatctgat ccgaacaaac tactgccaat cattaatgaa attttggata acaaccaaca
    14581 atctgtcgat gatttcaaaa atggaaaaga tcgtgcggtt ggcttcctag tcggtcaaat
    14641 catgaaagca actaaaggac aagcaaatcc tggtgtcgtg aataagctac ttaaagatga
    14701 attagcgaaa cgatagaggc tgaaaatgaa aaaagcacgc gtaatttata accccacctc
    14761 gggaaaagaa ttattgaaaa aaaatttggc ggatatccta caggcattag aagaagcagg
    14821 atttgaagcc agcgcctatg caactacacc agaaccagat tcagccaaga atgaagcatg
    14881 tagagtcgca gaactaggtt ttgatctagt agtggcagct ggtggcgatg gcacaatcaa
    14941 cgaagtggtt aatggaatcg ctccgcttga acaccgtcct aaaatggcca tcatcccagc
    15001 tggtacaacg aacgactatg ctcgagcatt aaaaattcct agagataata ttaaagcagc
    15061 agcagaagtg ataaaaaaac accaaaccgt aaaaatggac atcggccttt ctgataacag
    15121 ttacttcatc aatatcgctg caggtggcta tttgacagaa ttgacttatg aagttccatc
    15181 agaactaaag agtatttttg gttatcttgc ttatcttgcg aaaggagcag agatgctccc
    15241 tcgagtaaag ccaatcaaaa tgcgcttgaa gtatgatgaa ggggaatata tcggaaatgc
    15301 ttcgatgttt ttcctagggc tgacgaactc tgttggagga ttcgaacaga tcgcgccaga
    15361 tgccaaattg gatgatggga agttttcttt aatcattgtg aagacagcaa atatctttga
    15421 gatcttgcat attgcagcac tcatgctgaa tggcggtcga catgtcgatg atccaagaat
    15481 aatctatacc aaaacaagca gtttgttcgt tgaaacagaa gatgatatca atcgaccagt
    15541 aatgatcaac ttagatggtg aatacggcgg ggaagcaccg atgcatttcc aaaatcttca
    15601 tcaacacatt gagttctttg ccaataccga tcagattcct gatgaagcaa tcacaggtac
    15661 agatgaagaa gaattagagg ttgtcagcaa agaattcgta aaagaagtcg aacgtttaac
    15721 ggatgaagat atcgacggtg acggaaaaat agcagacgaa acacctaatc aaaagaatta
    15781 atcaaaagcc ggtgctgaac gtagtaaggt cagtgccggt tttagttgtg taattagctg
    15841 atttatagta gagttcagta ggattgaaaa ataggcttaa aggagcaaaa tatgacagaa
    15901 cagcaaatag taaagaaaaa cgaacgatat acagtcgaaa tcgtcgactt gagttatgaa
    15961 ggaatggggg tagcaaaaat cgatggttat ccattattta tcgaaaatgc actccctgga
    16021 gaagaagtag aaatactagt agtaaaagca ggcaataagt ttggatatgg aaaagtcgaa
    16081 acatttatta cacaatcacc tgatcgtcag cctgtagaaa acaatgcatt acttcgcata
    16141 ggtatcgcac ctttagccca tttgaagtat gaacaacagc ttgtcttcaa acaaaaacaa
    16201 gtagagcatg taatggcgaa gattgctaag atgccagaag ttcctgtact tccaacggtc
    16261 ggaatggcag aaccattcgg ctaccgtaat aaagcacaga tccctgttcg ccgtatcgat
    16321 ggacagttag ccaccggttt ttacaaaaag aatagccatg atctagtccc tatcgaagat
    16381 ttttatatcc aagatcctgt catcgatcaa gcaatcaaag tagtcagaga tatcttgcaa
    16441 cggttccaag tacgaggata taatgaagaa aaaaatgaag gacagcttcg tcacatcatt
    16501 atccgtcgtg gtcattattc ccatgaaatg atggtcgttt tagttactcg taaagaaaaa
    16561 ttctttaaat ccaaggaaat tgctgaagct attcatgaag aattgccaga agtggtttcg
    16621 gtcatccaaa atatcaatga agaaaaaaca aatgtgattt taggagacaa agaaaaagtt
    16681 ttatatggca gagactatat cgaagatcag ctattaggaa aaacctatcg aatctcagca
    16741 aagtcatttt atcaagtaaa tactcaacaa gcagagtatc tgtaccaaac agccatcgag
    16801 tttgccgagt tagataaaaa agacattgtg atcgacgctt attcaggaat aggtacgatt
    16861 ggcttgtctt tagcagacag agtggataag gtgtatggga tggaagttat tccagaagca
    16921 attgaagatg cacagttcaa tgctttaaat aataaaatcg aaaatgttca ttatgaagtc
    16981 ggaaaagcag aaaaagttat gaaaaaatgg caagaaaaag ggatcaaacc atctgtgatc
    17041 gtagttgacc ctccacgaaa aggattagat catcatttta ttgaatcagc aatcgaaatg
    17101 tcaccagaac gaatcgtcta tatctcatgt aatccggcaa cttttgcaag agatgctaag
    17161 ttatttgcag aagcaggtta tgccgtgaag aaagttcagc ctgtagatct tttcccgcaa
    17221 acccatcata tcgaattagt cgcttcattc gttaaaggag aataaaaatg gaccaaaaag
    17281 aaaaaatgaa agaattgatc aaaaagaaac aaggtggagg ccgatccaaa caaatggaaa
    17341 cacctaagaa tgatcgtaaa aacatgagaa aaggccctaa aatctttaat aaatagacgt
    17401 gctaaagagg ctctttgtca ataaggactg atgagttgtg caaaatcaaa tctgagtcag
    17461 aatgaacgtc attctgtctc agatttttcg ttatttcaat ttgattaact gattgatcaa
    17521 aaagattttt attttcatat tctcaaatga ttcaacacta aatatacatg tttgacgcgg
    17581 aaattattat tcgattttgg cggcatcagg ggggatgacg acgtagcaga cccattattt
    17641 tactcaagaa aatgtccgtg cctattattt agaatacgat agtgaacgtg ctgggagttt
    17701 tgttctttta gagaaagtct ctgaagataa attagttgtc ttagattttg actgtaacga
    17761 aaaatggcga aatggaagag caccagcaag tgattgaccg gatctacgaa gcgacacgat
    17821 atctgccttt ggatcgtttg tgcttaagtc ctcaatgtgg atttgcttca acagaagaag
    17881 aaaatgtttt gtttgaagaa caatggaaaa aatttctttt gtcaaaagca ttgcagaaga
    17941 agtttgggga aaatcattaa atcatctagg agagcttgat aggaaataac actatggtat
    18001 aatcagaatg cttaatacta actactgagg gagccttatg aaaaagaaaa ttcttaaatt
    18061 tatcggatgg ttcttcggaa tcattcttgg gatcgcgttt gcaatctttc ttgccttcca
    18121 gctttccccc agacctggag cattcttgat caaccatatg ttttcaaata ctgttcaaat
    18181 cactgataaa aaaacgttcg atcaagcaga aaaaaacgtg gcgaaaaaaa cagatttgac
    18241 gtatgagtcc aagtataaag agaatactta tgacatctac tatccaaaaa acagcaaggg
    18301 gcctgttcct gttctatttt gggtacatgg tggaggattc gtcggcggag ataaatcggg
    18361 tgtcaaagaa tttgcgacaa aattagtttc agacgccaac atcgcagtgg ttgcgatgaa
    18421 ttatgaatta gcgcatgatt cagaataccc taatcaagtt cttcaagtga atgaactgat
    18481 caaagattta ttagctgaag aaaaagacga caaattgcta gacatggaac aattattttt
    18541 tggtggagat agtgcaggtt cacaaatcgc attgcaatat gcagctatac aaacgaatga
    18601 tgcgtatgct aaacaaatga aaatcaaaca gctacttcca aaagatagtc tgaaaggaac
    18661 catttcttat tgcggacctg ttgatttgaa gctaacagca cagcagcatt cggatgatcg
    18721 atttatgaag tttttcgtca aaaccgttgc gtggtcgttg attggaacaa aagattggaa
    18781 aacgagtgag caattgaaag aagctagtgt ggctgaccat gtttctaagg aatttccgcc
    18841 aacgtatata actgacggaa atgcatactc attccaggag caaggtcaag cattagaaaa
    18901 tcggttgaca gaattaaagg tacccgtgca atccttgttt tatacagata caaaaaaaga
    18961 gattacccat gaataccaat ttgattacac gctaaaagaa tcgcaaaatt gttaccagca
    19021 gacattggat tttgtgaata agtataagta agaggttgtg aaaaagccgt ttcgttttga
    19081 gtatgaagaa acgaagcggc ttttcacaac ctctctttca ataccttttt gttcttggga
    19141 taaaactttc cctaacaatc aatcgtgcag gataagtcaa aatgattggt tcgtcccgta
    19201 taccgtcaat tcgctccaat gccattcgaa cagctgcttg cccaattgat ttcgcattga
    19261 tttgaacact ggtcaagcca ggggagaggt ttgcgattgt ctcgatatta tcaaagcttg
    19321 ctacaacaat ctcttctcca atttggatat ttttttgttt caatgcccga tagacaccaa
    19381 ttgccattgg atcgctcccg acaagaatgg cagtaggaag aggggattca ttttctaata
    19441 gattagtcgc aagtacctca ccttgttttt ctgtccagcc acctagatga taagacatat
    19501 atttttctaa gtgatgatcg atcataaaat gctgataggc acgcatcctc ttttcattta
    19561 tagaaatctg cttcccaccg aattcatcca tatcgacttt atatcccccg atataggcta
    19621 ttcttttatg accattttct aagaacaatt ccagtacggt tttagtcatc cgttcaagat
    19681 ctgcatacac catatcgacc tcttgctgga tgtcagggtt atccacgacg actatttgca
    19741 tgttttgttt taacaaggcg gtaatcgatt ctttcgtaac agtcccaaca acgatcaccg
    19801 cacctaactg atctagatct tcccattgtt taggattatc agatagatta tagattcgat
    19861 tcatccctaa atgcagacgt cgagcttctt cttccatccc tactcgcaaa tgtttgtaat
    19921 agatatcctg ttcttcgctc gctttgccga tcgtagagat cacaccaatc gaaggcattc
    19981 tctttttgat atatttacga gtactgatgt cataatccaa cgcagcagct gcctctacta
    20041 tccgttgccg agtagtctga gccacatgga tcgtctgatc gttattcaac acacgggaaa
    20101 ctgtagcagg agagacattt gcctttcgtg cgacatctct aattccaacc atttttacac
    20161 actcctcaaa aaaataaaaa atttttattg acaaaattgt ttagtaaaca gtatacagga
    20221 attataaaaa aaagtaaacg gtttccgttt tgttgggagt gaaatgatgg atattacgca
    20281 tagaaaaaca gataaaaatt tcatcttatt tgatgaaaag agccgagtct tccatttacg
    20341 aaatacattt ctttcttatc tgataaagat agaagaaagc aatgtattgg cgcatattta
    20401 ttttggcaaa ccagtcaagc agtacaaaga caataaaaat tatccccgaa gagatcgtgg
    20461 attttcagga aatgttccct taaatcctga ccggtctctg tcgaaagata cactaccgca
    20521 agaatactct tctcatggca gcatggactt tcgaacgccc gcatcgatca ttcagcgcaa
    20581 gaatggatca gacttactgg atctgagata tgacagccat tatattacag atgggaagcc
    20641 agagatcgaa ggcttgcctc aaacttatgt gatggacaag tcagaggcac aaacgttggt
    20701 gatttcactt aaggatagag aaacagcgat ttatttcgat ttgttctata ccatatttac
    20761 tgatagagca gtgatcacac gatctgtaaa gataagaaat gaaaccggag aaacaatcaa
    20821 actagaaaaa gcggctagtt tccagctaga ctttgcccat actaggcgat ttgatgaagt
    20881 cattgctttg ccaggagcac atgtcaatga aagacagatt tcacggcaat ctgttttatc
    20941 tggaacaaaa gtatttgaaa gccgtcgagg aacgagcagt caccatatga ataactttat
    21001 tgctttagtt catcatcata cgacagaaaa tactggagaa gcgatcggac ttcaatttgt
    21061 ttattccggc aaccattctt tcgaacttga aaaagatcag atcaatcaat tgagagtcgt
    21121 cggtgggatc aacagccatc gtttttcgtg ggaattgaat gcaggccagt cttttcaaac
    21181 acctgaaatg atcctttctt actcaagtca gggattgaac aaaatgagtc agatccacca
    21241 tgagttgcta cgtgaaagaa tcgcaagagg tcgtcatcaa tttgctgaac ggccgattct
    21301 ggtcaataac tgggaagcca cttactttga cttcaatagt gaaaaaatca aggcgatcat
    21361 tgatgaagca aaggagctag gcatcgaaat gttcgtatta gatgatggct ggtttggaaa
    21421 aagagatgcg gacaattctt cattaggaga ctggttcgag tatgaaggca agttgacaaa
    21481 tggattacgt gaaatagcag attatgctca tagcaaagga ttgaaatttg gtttatggtt
    21541 tgaacctgaa atgatctcgg ttgattctga actgtatcga acacatcctg attttcttat
    21601 gcaggagcca ggtagaatgc cttctgcttc cagatcacaa catgtactgg attttacaag
    21661 gttagatgtt cggcagacga ttgagaaaca aatgagaaag attttagata cgattcctct
    21721 tgactacatc aaatgggata tgaaccgttc gctttcagat gtctattcga ttacccttga
    21781 cccacagaga caaggagaag tagcgcatcg ttatatgctc gggctttatg aactacttga
    21841 acatttgaca actgattatc cggaaatctt atgggaaggc tgttctggtg gaggtggacg
    21901 gtttgatgcc gggtttattt attatatgcc gcagtcatgg acaagtgata acacggatgc
    21961 agtggagcgt atgaaaatcc aatatggaac aagccttgct tatccgattt cgtccatcac
    22021 agctcatgta tcagctgtac caaatcatca gaccgacaga tctacttcgt tgaaaacaag
    22081 aggagaaaca gctatgagcg ctgtttttgg ttatgaactg gatttgacaa agctgtctcc
    22141 tgaagaaaaa aagcaagtaa aagaccaaat tatatcctat caaacgattc gtccggtgat
    22201 ccagtatggc cattattatc gtttggcgag tccttttgaa gaaaatattg ccgcatggat
    22261 gtttgtgtct cctaaacaag atgaagctat tgtgttttta gggaggatac tagcttcagc
    22321 tcaaccagcg ttccatgaag tatatctgat ggggttagat gatgaggcac tttatcagga
    22381 acagacctcg aagcggatat tttcgggggc cgaattgatg acagttggac tttacttccc
    22441 cgattttcaa ggtgatttcc aaacagaact gcttcatttc aaaaagttat gagagagaag
    22501 gaaaaaagta tgaaagcaaa tataaaatgg ttagatgatc cacaagtttt tagagtgaac
    22561 caatcaaatg cacatagcga tcatgtttgt tatgcaacaa gggaagaagt agaaaccaca
    22621 tcctccctta aacagtcttt aaatggatca tggcgttttt gttattcgcc aaatccttcg
    22681 gtacgaccaa aaacatttta taaaaaagat tttgatgcct gttcttttga tgaaatcact
    22741 gttcctagtc atattgaatt agcaaattat gacaagatcc attacatcaa tacgatgtat
    22801 ccgtgggagg gacatgtatt tcgccgtccc cctttcacga caagccctga gaaagaggcg
    22861 gaagggatct tttcagctgc taaagacaat ccggttggtt cgtatttgac gacttttcga
    22921 ttagaagaag gtctaaaggg aaaacaaatc tcgattcatt ttgctggtgt agaacaagca
    22981 atgtttattt ggttgaatgg tcattttatc gggtatgccg aagattcgtt cactccgtct
    23041 gaatttgatt taacacctta tttggaagaa gaaaatattc tagctgtgga ggtttacaaa
    23101 agaagtacag ctgcattctt ggaagatcag gactttttcc gattttttgg gatcttcaga
    23161 gatgtcttct tagttgctcg aggacaaaac catttagaag atctttggat caaacctcgc
    23221 ttagatgaca aatgtcaaaa tggcaaactt gaagtagatt tgtcttttga gcaagtagcc
    23281 aaaaacagtc agctgaatgt agcgatacta gatcctgatg ggaaggaaat ctatcaacag
    23341 caatatccgt taaaagagca aatgacgatt cgcatcgatg gatttgaaaa tgtggcactg
    23401 tgggggcatt ttcagcccaa tttatataag cttgaattgg agacgataga cgaagaaaaa
    23461 agagtaacgg agtatattga ctattccttc ggttttcgta aaatcgaaat caaagataaa
    23521 atcatctact tcaatggaaa acggatgatt atcaacgggg taaatcgaca tgagtggcat
    23581 ccgaaaaaag gacgcgccat cactatagaa gatatggaaa aagatttggc tatcataaaa
    23641 aatgcaaaga tcaacggcgt acggacatct cattacccca accagatcac ttggtattat
    23701 ttatgcgacc aagcaggaat ccatgtgatg gcggaaacga acctcgaatc ccatggctca
    23761 tggcaaaaaa tgggcgcatt tgagccttct tacaacgtac caggtgcagt acctcagtgg
    23821 caggcggctg ttcttgatcg agcaaaaacg aatttcgaaa cattaaaaaa ccatccaagt
    23881 attctttttt ggtctcttgg aaatgaatct tatgcggaag aaaacattcg acaaatgaac
    23941 gattacttca aaaaagtaga ccccgatcga cttgtccatt atgaagggaa ttttccaaat
    24001 ccagcattcg aagattcgat ttcagatgta aaaagctgca tgtatgcgcc accagaagag
    24061 atcattgact accttgaaaa taaagcggaa aaaccgttca tcttatgtga atatatgcat
    24121 gacatgggga attcgttagg tggaatggat tcttacatga cgttgcttga tcgttatgaa
    24181 atgtaccaag gaggctatat atgggatttt aaagatcagg cgatccaagt gactgatcca
    24241 atcacaggca gagaagtgat gcgctatggc ggtgactttg acgataaacc aagtgactat
    24301 gaattctcag ggaatgggat cgtttttgca gatggacaag aaaaacccgc catgcaggag
    24361 gtaagatatt attatgaaaa atacagtaaa taaaagtcat atggatacgg aaaaagttgc
    24421 aatcgtcttc ggcgactgta cattaggtgt caaatcgggg aatacgcatt atattttttc
    24481 ttatacaaga ggcggactgg aatcgctcaa taaaaacgga aaagaatggc tataccggga
    24541 aacacttcca gttttttggc gagcaacgac cgataatgac agaggtagcg gatttggatt
    24601 taaatccggt atgtggttag gagccggatt gtatccaaaa gtcaaagaaa tagaagtact
    24661 ggtggacaat aaaaagatcg atttgccgat tgctccagta aataatcaat attcaagtaa
    24721 tgaatacgct gaaacggttg aaatcatttt tacactggaa atcaacacag taccaaaaac
    24781 ccaagtgaca atttcctacc aaatcgctgg agagggtaaa atgcttgtca atatgcatta
    24841 ttatggacaa aaaggattac cggaattacc cgcattgggt ttaaggttca ttatgccaac
    24901 gaaagcaaaa ttttttgagt atgaaggcct atcaggggaa acatatccgg atcggatggc
    24961 tggtggtgtc gaaggtattt ataaagtggc aggactcccc gtttccccat accttgttcc
    25021 ccaagaatgc ggcatgcata tggaaacgaa atgggtagaa ctaacaagaa atcaaacgtt
    25081 ggataatacg gataaagatc aagaagattt ttcgttgcga tttgaacagc aggatacgcc
    25141 atttgctttt tccgctttac cattcaaagc agaagaactg gaacaggcaa cacatataga
    25201 agaactaccg ccagaaagac gaacggttct tacgattttt gcaaaagtaa gaggagtcgg
    25261 gggtatcaat agctggggag cggatgttga gtcaccttac cgaatcagca gcgaagagga
    25321 ccatgaaatc ggctttatta ttcagtagca atctttagat tattatttga aaaacggagt
    25381 ttacaagaca aagctgcttt attctatatg tcatgaacaa gagagtaaaa aagttctcag
    25441 atacttttgt tcatgactat tttcttcaat aaaaccaatt gttatttttc tgaaaaacaa
    25501 agttaactat cagaaaatta aaaaaagttt cataaaaatg att
//
LOCUS       DAZKLD010000024        25503 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.024, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000024 DAZKLD010000000
VERSION     DAZKLD010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25503)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25503)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25503
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.024"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            58..1383
                     /gene="ftsA"
                     /locus_tag="ACG8KR_000834"
     CDS             58..1383
                     /gene="ftsA"
                     /locus_tag="ACG8KR_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HFQ0200230.1"
                     /translation="MAKTGMYVGLDIGTTSVKVVVAEYIDSQMNIIGVGNAKSEGINR
                     GIIVDIDKTVQAIQRAVRQAEEKAGIQIKGVSVGLPANMLEVENCQGMIAVNGDSKEI
                     TDEDVRNVASAALVRSIPPERQIVSILPQDFTVDGFEGIKDPRGMIGVRLEMYGLLFT
                     GPKTIIHNIRKCVENAGLIVNEMVITPLALTESILSDGEKDFGTIVIDMGGGQTTTAV
                     MHDKQLKFTNLDQEGGEFVTKDISIVLNTSFNNAEALKINYGDAYPERTSPDEEFPVD
                     VIGQSEPVKVDERYLSEIISARMEQIFNKAKDALDQIEALELPGGVVLTGGAASLPGV
                     VDLAQEIFGVNVKLYVPNHMGLRNPVFTNVISIVDYSANLSEVYQLAKIAVTGETSAA
                     RQMVVEQETTNTYESYEAPEETIYDEPEPKESGENVKNKIKGFFTNIFD"
     gene            1406..2647
                     /gene="ftsZ"
                     /locus_tag="ACG8KR_000835"
     CDS             1406..2647
                     /gene="ftsZ"
                     /locus_tag="ACG8KR_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HFQ0200231.1"
                     /translation="MEFSIDNNINDGAVIKVIGVGGGGGNAVNRMIEENVKGVEFITA
                     NTDVQALKNSKAETVIQLGPKYTRGLGAGSQPEVGQKAAEESEQSLREALDGADMIFI
                     TAGMGGGTGTGAAPIVAGIAKELGALTVGVVTRPFTFEGPKRGRFAAEGIARLKENVD
                     TLLIISNNRLLEVVDKKTPMLEAFREADNVLRQGVQGISDLITAPGYVNLDFADVKTV
                     MENQGTALMGIGVASGEDRVVEATKKAISSPLLETSIDGAEQVLLNITGGLDMTLFEA
                     QDASDIVANAATGDVNIILGTSINEEMGDEIRVTVIATGIDESKKERKSSRPARQAQM
                     QSPAQKTVLDMDQAKPTSSEEENSFGDWDIRREQNVRPRVDDSNFDNIEKKEFDTFNR
                     EETKSHGDDELNTPPFFRRKK"
     gene            2664..3341
                     /locus_tag="ACG8KR_000836"
     CDS             2664..3341
                     /locus_tag="ACG8KR_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HFQ0200232.1"
                     /translation="MIADNLQKIKQEIQDSCALVHRDPKEVTLVAVTKSVESAQAEEL
                     VRAGVMNCAENRVDKLLEKKQDLFDFPEIKWHLIGNLQRRKVKLIVNEIDYFHALDSL
                     RLAEEIQKRLEKELSCFIEVNVSGEESKHGISPDELLPFVESLAACTKIKVVGLMTMA
                     PKEASDEQIHKVFGTLKELRDLVQAKGYAHAPCSELSMGMSQDFQIAIEEGATFIRVG
                     TALFKDE"
     gene            3355..3954
                     /locus_tag="ACG8KR_000837"
     CDS             3355..3954
                     /locus_tag="ACG8KR_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HFQ0200233.1"
                     /translation="MPLLNRGAIANFFGLADEEEYEYQDYPETTKKAVQPTKKNTTQN
                     QSTHSVSAANTHEKQKTATSRPVTHESIQPAEKKKVIELHQSPTKTVAKERTASRTST
                     QPKANLAGKITVMEPRAYSEAMAIAKRIIAGEATLVNFHLVEEKQARRIVDFLTGTVY
                     ALDGDIKRVGDEIFLCTPTGMEIDSSTAQSFADANLFDL"
     gene            4035..4256
                     /locus_tag="ACG8KR_000838"
     CDS             4035..4256
                     /locus_tag="ACG8KR_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HFQ0200234.1"
                     /translation="MVYALLSWFPGGYQSGLGRLLAKICEPYISLFDRLPLRIGPLDF
                     TILGAILILNLAVQGLAQIVIWITVHLVY"
     gene            4305..5087
                     /locus_tag="ACG8KR_000839"
     CDS             4305..5087
                     /locus_tag="ACG8KR_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HFQ0200235.1"
                     /translation="MDANVYQHFRADERTFIDSVGDWIEQVQGQYAPYLTEFLDPRQS
                     YILETLIRQSSDLRFMFYGGYEQAERKRCLIFPDYYEPVEEDFETALYEIHYPSKFAS
                     LSHGKILGTLVGTGIKRSYFGDIISDGDRWQVFIANEVDHFVAAQVTKIGKVAVRLEP
                     IKYTEMIMPKDSWTQERGTVSSMRLDSVISEIYNISRQRSKQLIEAGKVKVNWTENTR
                     PDFVLELLDIISVRGYGRVQIQELEGKTKKEKYRVLFGVLRK"
     gene            5108..5815
                     /locus_tag="ACG8KR_000840"
     CDS             5108..5815
                     /locus_tag="ACG8KR_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HFQ0200236.1"
                     /translation="MALTPLDIQNKTFPTKMRGYNQDEVDDFLDLVVRDYEELTQRNR
                     ELEKAVKHSEEKLEYFNELKDALNQSIIVAQDTADKVKTSASKESEVIVTSAQNKADE
                     LVANAEKRAHQLTTDAEEKARKILTDATEKARQLATETEDLKKKTRVFHQRISLMLES
                     QLEQVKSPEWDEILQPFSSYVTDSHEVIKEVLAKQLDNENDTDVNSDTGVIEAPMTAF
                     DTQAIDISIVPGENENE"
     gene            6077..8875
                     /gene="ileS"
                     /locus_tag="ACG8KR_000841"
     CDS             6077..8875
                     /gene="ileS"
                     /locus_tag="ACG8KR_000841"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HFQ0200237.1"
                     /translation="MKMKETLQLGKTKFPMRGNLPNREGEWQKEWEENRIYQKRQELN
                     EGKPSFVLHDGPPYANGNIHLGHSLNKISKDIIIRSKSMSGYRAPYVPGWDTHGLPIE
                     QVLANKGVKRKEMSMAEYLEKCREYALSQVDKQRADFKRLGVAGDWEHPYVTLDPSYE
                     AAQVRVFGKMAEKSYIYKGLKPIYWSPSSESSLAEAEIEYKDVKSPSIFVAFNVKDGK
                     DILDEDTAFVIWTTTPWTLPANQGIAVNPKFTYVLVEADGRKFVVAKDLLETVQSEIG
                     WENVTVLKEFAGQEMEYMTASHPFYERESLVILGDHVTLDAGTGLVHTAPGHGEDDYI
                     AGSKYKLSVVSPVDSKGLFTEEAPGFEGIFYDKANPMITDLLKEKGALLKLDFFTHSY
                     PHDWRTKKPVIYRATPQWFASIDKFRQNILDEVEKVEWVIPWGKTRLYNMIRDRGDWV
                     ISRQRAWGVPLPIFYAENGEAIITPETIEHVAKLFAEHGSIIWFEKEAKELLPEGFTH
                     PGSPNGEFTKEKDIMDVWFDSGSSHEAVLRQRPELSFPADMYLEGSDQYRGWFNSSIT
                     TSVAINGVAPYKSVISQGFALDGEGRKMSKSLGNIIAPEKVIKQFGADILRLWVSSVD
                     YEADVRVSMDILSQVAEVYRKIRNTMRFLLANTEDFDPKKDTVSYNDLRSVDKYMTVR
                     LNQVIKEIREEGYDKYNFMHIYRTVMNFLTVDLSSFYLDFAKDVVYIEAEDDYQRRCM
                     QTVFYQTAVALTKLLTPIIPHTAEEIWSFLKEEEEYVQLSEFPGYEEFANQEELMDTW
                     TAFMDFRDNVLKALEEARNSKLIGKSLEAKLTVYPKQQVREMLKALDADIAQLLIVSP
                     DYFEVMAADEQVPDNAMVFDDVAITVEKADGETCDRCRQVRKDVGVDEKLPHLCGRCA
                     KIVEDFYPEAVAEGFEEK"
     gene            complement(8924..10450)
                     /gene="zwf"
                     /locus_tag="ACG8KR_000842"
     CDS             complement(8924..10450)
                     /gene="zwf"
                     /locus_tag="ACG8KR_000842"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HFQ0200238.1"
                     /translation="MSNEKNVLFTIFGGTGDLAQRKLYPSLFRLYRKGNLGEHFAVIG
                     TARRPWSDEHYREVVKETIRALNPSDEEATTFASHFYYQSHDVNDSTHYQTLKELSDR
                     LDGHYQLNGNRVYYLAMAPQFFGTIVSHLKSQNIMTKNGFNRLIIEKPFGSDYQSAFE
                     LNQQIREVFPEQDIFRIDHYLGKEMIQNISAIRFANNIFEAQWNNRYIDNIQITFGEN
                     LGVEDRGGYYDHSGALKDMVQNHILQVVALLAMEPPVAFSEKEIRTEKIKALKAIRIY
                     NEEEVLENFVRGQYAQGELDGVQFKGYREEDKVDAQSETETFVAGKFTIDNFRWSGVP
                     FYVRTGKRLTEKGTRINIVFKQVPVNVFKTSVDEPCDDTTLPPNVLTIYIQPTEGFSL
                     SLNGKEVGQGFETEPIKLEFRNSAEMVENSPEAYEKLLLDALNGDGTNFSHWEEVAQS
                     WHIVDVIRKAWDQTQPDFPNYKAGSMGPKAAFELLEKDGFEWIWQPDNWYRERGKLND
                     "
     gene            complement(10465..11112)
                     /locus_tag="ACG8KR_000843"
     CDS             complement(10465..11112)
                     /locus_tag="ACG8KR_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HFQ0200239.1"
                     /translation="MTPNREDYLKIILELGGDTTKVNNKQIVSSLDVSAASVSEMISK
                     LVKEELVEHSPYQGVQLTASGLQKASSLIRKHRLWEVFLVEHLDYSWNEVHDDAEVLE
                     HVTSEHLADHLENYLNHPEHCPHGGSIPKKEEVVHEERRQTLESYPVGTKVRIARVLD
                     EKELLDYLVTIDLNINEEYEIVDIAAYEGPITIQNDRKKIPVSFKAASTIFVDKI"
     gene            complement(11296..11625)
                     /locus_tag="ACG8KR_000844"
     CDS             complement(11296..11625)
                     /locus_tag="ACG8KR_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4828 domain-containing protein"
                     /protein_id="HFQ0200240.1"
                     /translation="MIYFLGSLITVLSSFIYLKKKKQAEKSEGLSKYQGTWFFSDSQQ
                     KQQLQITSDYTFLINGQSLGTSVVEIDEYRLVVRDQYGFYLIFSYPEQTLYDESADTS
                     YKLLSEK"
     gene            11802..12530
                     /gene="sfsA"
                     /locus_tag="ACG8KR_000845"
     CDS             11802..12530
                     /gene="sfsA"
                     /locus_tag="ACG8KR_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HFQ0200241.1"
                     /translation="MKYQQVEIAFFIDRPNRFIAHCMKKDGEIIIAHVKNTGRGKEVL
                     IPGAEVAIVFAPGPKRKTAYDLIAVKKKGDWFNIDSQLPNRLAIDGILDGTILLPGLT
                     GPFTVYKREVTYLHSKFDIYLETVVGQKAFVEVKGMTLENKAIGAFPDAPTLRGLKHI
                     GELTYAAQDGYAAYVLFIAQFEHLHLATIHEEMQPALADMVRHAQQSGVQILAYNCQV
                     SADEVTVKQAIPFDVDIPFEDPIQ"
     gene            12546..13559
                     /locus_tag="ACG8KR_000846"
     CDS             12546..13559
                     /locus_tag="ACG8KR_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFQ0200242.1"
                     /translation="MINLGIIGTNWITHQFVQAALSTKKYELTAVYSRHLEKAQEFGS
                     HYEGDIEFATDLKTFFNITHMDTIYIASPNSLHFSQAKQAILAGKNVIVEKPAFSTPK
                     EMDEIIHLANEHNVYFFEAARNIHESGFKKIADYLPLKDEIIGANFSYMKYSSRYDQV
                     LDGEEPNIFSTHFSGGALMDLGVYLVYAAVAWFGMPKEVHYFPRKISTGVDGLGWGIL
                     RYENFDVSIQPGKIGDSYLPSEIYFDSGTLVMNGVNAIEQAQFHDRAHKEIIDLAVTA
                     AENPMADEAEDFANVMAHPKDPAWGLLYEEWIELARNVNQVIYDLRKNGGIKFDADNE
                     EDF"
     gene            13559..14836
                     /locus_tag="ACG8KR_000847"
     CDS             13559..14836
                     /locus_tag="ACG8KR_000847"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFQ0200243.1"
                     /translation="MNEFVQVFPENWQKKYEDKGFISPSLIQRAVFQPLSNKQSIVAV
                     SPTGSGKTLAYLWPLLLNVEPGEASALVIFASSQELAIQVADVAREWGKDKELKVQSL
                     VGGANVKRQIEGLKEKPEILIGTPGRILELMKAKKIKAHQVKTMVFDEADQLFDAGNS
                     QIIDQILHQAPTEYQLAFFSATADRSLESIEKITGKTFETIDVTAEDDSRKGLRHYFL
                     RVPIRKKDDYLRRLTHIEDFHGLIFFNQLTELGVMEEKLLYRGVPVGSLASDQNKLLR
                     KAALEQFKKKMVRALLTTDVAARGLDITGLPYVVNAEVPLSEEAYLHRSGRTGRMGNE
                     GIVITLVQDNNLRDLKRLLRPSNITLEEIFLYGGKLQTEQPTKEDAAPAVNKKYAKTF
                     SGKEQNPEYNKEKIRKKVKNKSKKERNKGARRK"
     gene            14899..17601
                     /locus_tag="ACG8KR_000848"
     CDS             14899..17601
                     /locus_tag="ACG8KR_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFQ0200244.1"
                     /translation="MKERQEEILQLLTENQQGLTASDVAERLTIDRSNASRYLSELYQ
                     AHHIVKTAGRPVVYSLPTEKSKSDEVHVDSSTQVTFETLVGENDSLKVSIQQAKAAIL
                     YPPRGLHTIIFGETGTGKSMFAECMYHFAIDSEMLSADAPFVSFNCADYAQNPQLLFG
                     HIFGIKKGAYTGAAQDSPGLIAKADGGILFLDEIHRLPPEGQEMLFTFIDKGIYRPLG
                     ESAQVHEASVQIIGATTESSESFLSTFNRRIPMAITLPNLASRSLDERYEIISLFIKQ
                     EANRLNQRIDIEKEAILAFMLYDAEGNIGQVKRDLKLVCAKSFLHYRTHHEKKLIIRK
                     EELSLQVQKGLLKIKEIPERLDRFMDSKSQYLTFEPGFADVVWSQDPERNMQVYNDIE
                     EKVTSLSETGVENIDLETLISKDVDAYFQTYVKELTKSSIPKDLLPDEIWRLTNQLYD
                     VAEKRLDRIYNEKARFAFALHLQSTLDRVREGHMIVHPDLNNVRKNFKREFQVAIDLS
                     TIIEEEEQVEIPFDEIGFISMFLSINLGESEPVKENKVDVLILMHGRQTASSMLEMAQ
                     ELLGTKVGKAFNMPLVMEVQTMYEEVLQYVKNYQEHLTNGLLLLTDMGSLNTFSDLIY
                     EETGIRTKAITMTSTMIVIEAIRMASVGRSLEDIYQNIQLSFESIVRDQFKAEKSETL
                     QRKKAVIVTCFTGEGVAAKLYQRIMPVINQNKVELIQMQFLERESFKKHIDALLEEYE
                     IKAIAGTVDVDYQNIPFFSAYDVFDDEKLNILKRIADDEIPTAKIVDSLAGSLSDVFS
                     IDELIRSLQKTVQQIQTDLSIIVEPGVDTGIVIHLAFMLDGLIKQESHRQFEKLSIFS
                     KIYRLEMDIVRANLMALERKYQVKIPEDDIAFLTQMFLENKIKK"
     gene            17753..18070
                     /locus_tag="ACG8KR_000849"
     CDS             17753..18070
                     /locus_tag="ACG8KR_000849"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFQ0200245.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEEKGMEADIFAVSASDA
                     DNNLESKNVDVLLLGPQVRFMKAQFEQKLAPKGIPLDVINMQDYGMMNGEKVLAQAEN
                     LMK"
     gene            18100..18420
                     /locus_tag="ACG8KR_000850"
     CDS             18100..18420
                     /locus_tag="ACG8KR_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFQ0200246.1"
                     /translation="MEDQQNLEAIMGLIMFGGNAKSDAMEAIAAAKIGDFELADRKIA
                     DAEESLIQAHHAQTDMLTQEAQGNHMQVTLLTVHSQDHLMTSIAFTDLAKEIIELYRR
                     VYSE"
     gene            18528..19988
                     /locus_tag="ACG8KR_000851"
     CDS             18528..19988
                     /locus_tag="ACG8KR_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFQ0200247.1"
                     /translation="MNGLSSWMEKYILPVAAKIGAQKHLVALRDAFIGTLPATMAGSV
                     AVMINAIIRDLPQQFNSTYADSLAADKGIFAVLNVIIGINGFVWNGTLAIAGLIFAFS
                     WGYNLARAYGVNDLAGGIVGLATLIQGISFGPALSAALDVNVPSNVADAINSSEIGAT
                     IADGTLSVGLWGWLKLDHLNGNAYFTVMIMGAISVIIFCKLMLANITIKMPDSVPPAV
                     SKAFAAILPATIALYVIAIINYIVGRVADGQLIIDLVQKYIAEPFLGLSQGLGAVLIV
                     TVFVQVFWFFGIHGPNVLAPVLEGIWGQAQLINIDIFQKGYNGKTGTPAVLDAIEDGK
                     AYMWVRGSFDAFAWFGGSGGTIVLLIAILIFSKRADYLTVAKLSIGPGIFNINEPVMF
                     GLPIVLNAIMFIPFLIAPVVATAIGWTATYLGLVAPVSQQVTWVVPPFLLSFLATGAD
                     WRAPIVTLVCMVVTFLIWAPFVISANKMDPAAGEQQ"
     gene            20056..20277
                     /locus_tag="ACG8KR_000852"
     CDS             20056..20277
                     /locus_tag="ACG8KR_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0200248.1"
                     /translation="MITIASAMQQDEIKELLEGYNEEGMTFTFKEKQGIKLFFETSAE
                     DPEVAAKKAKELIKAQPWGTVLYFQSTAV"
     gene            20308..20490
                     /locus_tag="ACG8KR_000853"
     CDS             20308..20490
                     /locus_tag="ACG8KR_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3188 domain-containing protein"
                     /protein_id="HFQ0200249.1"
                     /translation="MIKNGLFLCSIGSLIVLYSLNPHSQRYDLYSMVTGLFLTGLGIY
                     FFMKGKKREEAKEKNN"
     gene            20490..20903
                     /locus_tag="ACG8KR_000854"
     CDS             20490..20903
                     /locus_tag="ACG8KR_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HFQ0200250.1"
                     /translation="MEITKKLNIPASFFYDKVMDSVLFDVRKATGKSVTRKQLKNLEY
                     VKQFSQNNRARIKIEEVIENQSYKFRTSTTKNEFVVHYQIKPLDDSTCEVHYTEKMES
                     YGMLQKLNDMLLGTILMYFKRRRFKKMLDMIEATY"
     gene            21026..22207
                     /locus_tag="ACG8KR_000855"
     CDS             21026..22207
                     /locus_tag="ACG8KR_000855"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0200251.1"
                     /translation="MTNKVRELGVSSIEEMFDLATYAFNGADTPERRERFRTLAENSW
                     NYGFFDEEDRLTSQVMATPFPVNFYGQEYLMAGIGYVASYPEARGQGGINQIMEKILE
                     DCRDRKVSLSYLAPFSYPFYRRYGYEQSFDKISYQLNSRDVPFIKKKSGKIKRMDFED
                     AKAAIRRIYEQMPENQRAGLKREDWWYTYKFKQKGNYQFALHFDDNEEATGYIVYQLA
                     TSSFIIAEWGFLDHDAFCSLVRFVSSHNGAFETFEYSCGYGGNDQNYLLENPFASVMI
                     TPYMMARIVDISLFLENYPFKKRQAAFALQIEEDHYAKWNEGIVEVTIENGKNHLSKV
                     EQTKLPIVRGSIQHITQLLLGYRKVEELVFQERIQVTKEYEETLSSILPQQQPILNDY
                     F"
     gene            complement(22278..22442)
                     /locus_tag="ACG8KR_000856"
     CDS             complement(22278..22442)
                     /locus_tag="ACG8KR_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0200252.1"
                     /translation="MNDKPKEKVYAERLDSSKKNREKITKEAIQDHKKENPKTKENKK
                     FKFTKKEDLL"
     gene            22596..22669
                     /locus_tag="ACG8KR_000857"
     tRNA            22596..22669
                     /locus_tag="ACG8KR_000857"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:22630..22632,aa:Arg,seq:cct)
     gene            complement(22738..23877)
                     /locus_tag="ACG8KR_000858"
     CDS             complement(22738..23877)
                     /locus_tag="ACG8KR_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFQ0200253.1"
                     /translation="MARLVKRGNSWQYEISYKKDDGKYTKIRKSGFKTKGEAKDAANE
                     LEYNLNKGLKGDRKNLLLSDYFEDWMQLYKEGTVSPITYRKYEDTLMNIKKYMPAVLI
                     SDLDRVGYQRFLNKYAKDHVKSTVIKFNNHIRASLKDAVEEGLIPFDPTRKAVIKGKD
                     SLKPKEDKYLDYDQFKSLMKLVEENLSAQYSSPMLVLVAGATGMRFAELLGLTWEDID
                     FEDQIITINKTWNYKLNEWGKTKNETSNRKISIDKHTIDLLKKFKINQKELFENFEIK
                     NPHNFVFFNLKNGLVSSNAVSKYLRKKLKELGIEKQFTLHGLRHTHASILLYQGVNIL
                     SVSKRLGHSSLETTMSTYLHIVRELEDQDKEKINTVFDSLYKNDN"
     gene            24049..24183
                     /locus_tag="ACG8KR_000859"
     CDS             24049..24183
                     /locus_tag="ACG8KR_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0200254.1"
                     /translation="MIKVIFKRITELPEFRLFVDYWYVWFFIVALLIGLDVILERKLK
                     "
     gene            complement(24176..24811)
                     /locus_tag="ACG8KR_000860"
     CDS             complement(24176..24811)
                     /locus_tag="ACG8KR_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0200255.1"
                     /translation="MDNSNEKLTKKEYLELIVEGGIGAIPYIGGVVQSLYFGSKNEKR
                     FKRIEKFYKNLNESLENIRDQIPEDVFNLENKDQLIGIFEGINDEVEKSKAQNKIDMY
                     KNLYKNCLLRINNASWDNEEYYLLVLNQLTSIEIQLLACLMSRGNENFTGNISMSGYS
                     QELIDGSLNRLSDFGLLEKTISSIVLGDVGKQNMGYKISHLGIQFINSTLT"
     gene            complement(24924..>25503)
                     /locus_tag="ACG8KR_000861"
     CDS             complement(24924..>25503)
                     /locus_tag="ACG8KR_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3221 domain-containing protein"
                     /protein_id="HFQ0200256.1"
                     /translation="LIVRFIKKTNKKTPMILLIIGIIFTTVGFSLSSSNEANDNNKQE
                     SSSSDTQTTTSNSSNKSLTQFEEFLETNNHDWGAFLDSYYSITPATEQNTAFTKYISG
                     KTYTFEGTVIESMTTRIAIIADKEYDDKSWNDISATPKVSYVIFAKDLNNADTFTKGD
                     KVTFTGEISSRGSNIEKSYAQWDMINSKVSKK"
BASE COUNT         8249 a         4397 c         5264 g         7593 t
ORIGIN      
        1 aacggacgct agttgttcgc ttgtatatgg aatagtaagt aggagggaat cccattcatg
       61 gcaaaaacag gaatgtatgt aggccttgat attggaacga catctgttaa agttgtcgtg
      121 gctgaatata tcgacagcca aatgaatatt attggagtag gaaacgcaaa atcagaaggg
      181 attaaccgag gcattatcgt tgatatcgat aaaacggtcc aagcgataca acgagcagta
      241 cgacaagcag aagaaaaagc aggtatccag atcaaaggcg taagtgtcgg tttgccagca
      301 aacatgcttg aagtagaaaa ttgccaggga atgattgcag ttaacggaga ttcaaaggaa
      361 atcaccgatg aagatgtcag aaatgtggct tcagctgccc ttgttcgttc aatacctcct
      421 gaacgtcaaa tcgtgtcaat attgccacaa gattttactg tcgatgggtt cgagggaatc
      481 aaagatcctc gtggaatgat cggcgtaaga ttagaaatgt acggtctgtt gtttacagga
      541 cctaaaacga tcatccataa catacgtaaa tgcgtggaaa atgccggatt aatcgtcaac
      601 gaaatggtga ttactccttt agcgctgact gaatcaattc tttctgacgg agaaaaagat
      661 tttggtacga tcgtcattga tatgggtggc ggccaaacaa caacggctgt tatgcatgat
      721 aagcaattga agtttaccaa tcttgaccaa gaaggtggcg aatttgtcac taaagatatc
      781 tctattgtat taaatacgtc atttaacaat gcagaagcgt tgaaaataaa ctacggggat
      841 gcatacccag aacggacatc accagatgaa gaatttccag tagatgtgat tggtcagtca
      901 gaaccagtga aagtcgatga acgctacttg tcagaaatta tttcagctcg aatggaacag
      961 attttcaaca aagcaaaaga tgcattggac caaatcgaag cattagagtt gccaggtggc
     1021 gtcgtattga caggcggagc tgctagtctt cctggtgttg tggacttggc acaagaaatc
     1081 ttcggcgtaa atgtaaaatt gtatgttcca aatcacatgg ggctgagaaa cccggtattt
     1141 acaaatgtaa taagtatcgt ggattattca gcaaacttga gtgaagtgta tcaattggca
     1201 aaaattgctg ttacgggtga aacctcagca gctcgtcaaa tggtagttga acaagaaaca
     1261 acaaacacat atgagagcta tgaagcacct gaagaaacaa tctacgatga gccggaacca
     1321 aaagagtccg gagaaaatgt aaaaaataaa atcaagggtt tctttacaaa tatttttgat
     1381 taattcatcc tgggaggaat aagatatgga attctcaatt gataataata tcaatgacgg
     1441 cgcagtaatc aaagtcatcg gagtcggtgg tggcggtgga aatgctgtaa accgaatgat
     1501 tgaagaaaac gttaaaggtg ttgaattcat tacagccaac acagacgtgc aagcattaaa
     1561 aaattcaaaa gctgaaacag ttatccaatt aggccccaaa tatactcgtg gtttaggtgc
     1621 tggttctcag ccagaagttg gacaaaaagc ggcagaagaa agtgaacaat ccttacgcga
     1681 agctttagac ggtgcagata tgattttcat cactgcagga atgggtggag gaaccggtac
     1741 aggtgctgca ccaatcgttg caggtatcgc gaaagaattg ggcgcattga ctgttggtgt
     1801 cgtgacacgt ccattcactt ttgaaggacc gaaacgtggc cgttttgctg cagaaggtat
     1861 cgctcgtttg aaagaaaatg tggatacatt attaattatc tcaaataacc gtttattaga
     1921 agttgtagat aaaaagacac cgatgcttga agctttccgt gaagcagata atgttttacg
     1981 acaaggtgta caaggtatct ctgatttgat cacagcgcca ggttacgtta accttgactt
     2041 tgctgacgtg aaaacagtga tggaaaacca aggtacagca ttgatgggga tcggtgtggc
     2101 tagtggagaa gatcgcgtag tagaagcaac gaaaaaagca atctcctctc cattgcttga
     2161 aacatctatt gatggagctg aacaagtact tcttaacatc acaggcggtt tggatatgac
     2221 attgttcgaa gcacaagatg cttcagatat cgtagcaaat gcagcaactg gcgatgtaaa
     2281 tatcattcta ggtacatcta tcaatgaaga aatgggcgat gaaatccgtg tgacggtcat
     2341 cgcaacaggt atcgatgaat caaaaaagga acgcaagtct agccgaccag ctagacaagc
     2401 acaaatgcaa tcacctgctc aaaagactgt attagatatg gatcaagcta aaccaacttc
     2461 ttcagaagaa gaaaacagtt ttggcgattg ggatatcaga cgtgaacaaa atgttcgtcc
     2521 tagagttgat gattccaatt ttgacaatat cgaaaaaaaa gaatttgata cattcaaccg
     2581 cgaagaaaca aaatctcatg gcgatgatga attaaatacg ccaccgtttt tccgtcgtaa
     2641 aaaataaagg agaggaagag cacatgattg ctgacaactt gcagaaaatc aagcaagaaa
     2701 ttcaggattc gtgtgctctt gttcatcgtg atccgaaaga agttacatta gtagctgtaa
     2761 caaaatctgt tgaatccgct caagctgaag aattggttag agctggcgtt atgaattgtg
     2821 cagaaaaccg agtagataaa ttattagaaa agaaacaaga tttatttgat tttcctgaaa
     2881 taaagtggca tttaattggt aatttacagc gcagaaaggt aaaattgatt gtaaatgaaa
     2941 ttgattactt tcatgcgttg gacagtttaa gattagcaga agaaatccag aagcggttag
     3001 agaaagaatt atcctgtttt attgaagtaa atgtttctgg tgaggaatca aaacatggaa
     3061 tttcaccaga tgagctttta ccgtttgtgg aatctttagc tgcctgcaca aaaatcaagg
     3121 ttgttgggtt gatgactatg gcgcctaaag aagcttctga tgagcaaatc cataaagtgt
     3181 ttggtacgct gaaagaattg cgtgatcttg ttcaagcaaa aggttatgcc catgcaccat
     3241 gtagtgaatt aagtatgggg atgagtcaag actttcaaat tgcgattgaa gaaggtgcaa
     3301 catttatccg tgtaggaaca gcgctattca aagacgaata aaggaggaac aaaaatgccg
     3361 cttttaaaca gaggagcaat tgctaatttt ttcggattag ctgatgagga agagtacgaa
     3421 taccaagatt atcctgaaac aacaaaaaag gctgtacagc caactaaaaa aaatacaaca
     3481 cagaatcaat caactcattc tgtatctgct gcgaatactc atgaaaaaca aaaaacggcg
     3541 acttcacgtc ctgtaacgca tgaaagtatt cagccggctg aaaagaaaaa agtaattgaa
     3601 ctacatcaat cacctacaaa aactgtagcg aaagaaagaa cagcttcaag aacctctaca
     3661 caacctaaag caaatcttgc tggaaaaatc actgtaatgg aaccaagagc ttattctgaa
     3721 gctatggcga ttgctaaacg aatcattgct ggagaagcaa ccttagtgaa ttttcattta
     3781 gtagaagaaa aacaagcgag aagaatcgtt gattttctaa caggaacagt ttatgcacta
     3841 gatggagata tcaaacgtgt tggagatgag atttttcttt gtacaccgac tgggatggag
     3901 attgacagtt caaccgctca atcttttgca gatgcgaatt tgtttgatct gtaagaggag
     3961 gaactacgat ttatactatt gcactattac ttcatttact tcaacgggca gcctatttct
     4021 atagcattat cttgatggta tatgcattgc tctcttggtt tccaggcggg tatcaatcag
     4081 ggctaggacg attattagct aagatctgcg aaccgtatat cagtttgttc gatcgtctgc
     4141 ctttgaggat tggacccttg gatttcacaa ttttaggggc aatcctgatc ttgaacttag
     4201 ccgtacaagg tttagcacag atcgttatat ggataactgt ccatttagtt tattgattga
     4261 agcaacaaaa tctttattct cagaatagat aggaggggaa aattttggat gcaaatgtat
     4321 accagcactt tcgggcagat gaacgtacat ttatcgattc ggtaggcgat tggattgaac
     4381 aggttcaagg acaatatgca ccctatctga ccgaatttct tgacccaaga caatcctata
     4441 ttttagaaac actgattcga caaagcagtg atttgcgttt catgttttat ggcggatatg
     4501 aacaggcaga aagaaaaaga tgtttgattt tccccgatta ttacgaacct gtagaggaag
     4561 attttgaaac agcattgtat gaaatccatt atcctagcaa atttgccagc ctaagtcatg
     4621 ggaaaatcct tggaacttta gtgggaacgg ggatcaaacg ctcttacttt ggcgatatca
     4681 tctcggacgg tgatcgctgg caagtattta ttgcaaatga ggtggatcat tttgttgctg
     4741 cacaagtaac aaagattgga aaggttgctg tacgcttgga accaatcaaa tacacagaaa
     4801 tgattatgcc gaaagatagc tggacacaag aaagaggaac agttagttcc atgcgactag
     4861 acagtgtgat ctctgagatt tataatattt ccagacagcg gtccaagcag ctgatcgaag
     4921 ctggtaaagt aaaagtgaac tggacagaaa atacaagacc tgattttgta cttgaactat
     4981 tagatattat ttcagtcaga ggttatggca gagtccagat ccaagaactt gaggggaaga
     5041 cgaaaaaaga aaagtacagg gtactgttcg gtgtattacg taaataaaat aaaaaggtgg
     5101 tttactcatg gctttaactc cattagatat ccaaaataag acattcccga caaaaatgcg
     5161 gggatataat caagacgaag tagatgattt cttagattta gttgttcgtg actatgagga
     5221 attgactcaa agaaatcgag aattagaaaa agcagtcaaa cattctgaag aaaaacttga
     5281 gtacttcaat gaattaaaag atgctttgaa ccaatcaatc atcgtagctc aagatacggc
     5341 tgataaagta aaaacaagtg caagcaagga atccgaagtg atcgttactt ctgcacaaaa
     5401 caaagcagat gagttagttg cgaacgctga aaaacgtgca catcaattaa caactgatgc
     5461 agaagaaaaa gcaagaaaaa tcttgacaga tgcaacagaa aaagcaagac agcttgcgac
     5521 agaaactgaa gatcttaaga aaaaaacacg agtattccat caacgtatca gcttgatgct
     5581 tgagtctcaa ttagaacaag tgaaaagtcc ggaatgggat gaaattttac aaccattctc
     5641 cagctacgtg actgacagcc atgaagtgat caaagaagtt ttagcaaaac agcttgacaa
     5701 tgaaaatgat actgatgtaa actcagacac aggagtcata gaagcaccta tgacggcatt
     5761 tgatacacag gctattgata tctcgatcgt gcctggtgaa aatgaaaacg agtagaacag
     5821 aaaagcactt tgttattctt aaagcgagcc gatgagagtg gaagttcggt aagacactgc
     5881 atggtgaaag atcctctact tgagacctgc ttttccgaaa ttagagtaag aagcagcggc
     5941 tgacctcgtt attggtcaca gagagtttgc actagataga tgcaactaaa tttgggtggt
     6001 accacggcat gttcgtccct tgcggctgaa catgcttttt tgtgtccgga aaacaaataa
     6061 aggagtgtat ggttgaatga aaatgaaaga aaccttgcaa ttaggaaaaa caaaattccc
     6121 tatgcgtggt aatttgccaa accgagaagg cgagtggcaa aaagaatggg aagaaaatcg
     6181 aatctaccaa aaaagacaag agttgaacga aggaaaacct agctttgttc ttcatgatgg
     6241 acctccatat gccaatggta atatccattt aggacattca ttgaataaaa tcagtaaaga
     6301 cattatcatt cgttcaaaat caatgtctgg ctatcgtgca ccatatgttc caggatggga
     6361 tacacatgga ttgccgattg aacaagtatt ggctaataaa ggcgtgaaac gtaaagaaat
     6421 gtccatggca gaatacttgg aaaaatgtcg tgaatatgca ctttcccaag tagataagca
     6481 acgagcagat ttcaaacgcc taggagttgc aggagattgg gaacatcctt atgttacatt
     6541 agatccttct tatgaagctg cacaagtacg cgtatttggt aaaatggctg aaaaaagcta
     6601 tatttacaaa ggattgaaac caatttactg gtctccgtca agtgagtctt ctttagcgga
     6661 agctgaaatt gaatataaag atgtaaaatc tccatctatt tttgtagctt tcaatgtcaa
     6721 agacggcaaa gatattcttg atgaagatac agcttttgtc atttggacga caacaccatg
     6781 gacattgcca gcaaaccaag gtattgctgt aaatccaaaa tttacgtatg tattggtgga
     6841 agccgatggc cgtaagtttg tcgtagcgaa agatttgcta gaaacagtcc aatcagaaat
     6901 cggttgggaa aatgtaacag ttctaaaaga atttgctgga caagaaatgg aatatatgac
     6961 tgcaagtcat ccattctatg aacgtgaatc acttgtgatt ttaggcgatc atgttacttt
     7021 agacgctggt actggattag tccatactgc tccaggacat ggggaagatg actatatcgc
     7081 tggtagcaaa tataaactat ctgtagtgtc acctgtagac tcaaaaggat tgtttacaga
     7141 agaagcacct ggatttgaag gtatctttta tgacaaagca aatcctatga tcactgatct
     7201 attaaaagaa aaaggagctt tgctgaagct agatttcttt actcatagtt atcctcacga
     7261 ttggcggacg aaaaagcctg tgatctatcg ggcaactcca caatggtttg catctatcga
     7321 taaattccgt cagaatattt tggatgaagt agaaaaagta gagtgggtta tcccatgggg
     7381 taaaacacgt ttgtataata tgatccgtga ccgcggcgat tgggtaattt cccgtcagcg
     7441 tgcatgggga gtaccacttc cgattttcta tgctgaaaat ggagaagcaa tcatcacacc
     7501 agaaacaatc gaacatgtgg caaaattatt tgctgaacat ggatcaatta tctggtttga
     7561 aaaagaagcg aaagaacttc ttcctgaagg atttacccac ccaggttctc ctaatggcga
     7621 atttacaaaa gaaaaagata tcatggatgt ctggtttgat tctggttctt ctcatgaagc
     7681 tgttttacgt cagcgaccag aattatcatt cccagcagat atgtatttag aaggatcaga
     7741 ccaatatcgt ggctggttca actcaagtat cacgactagt gtggctatca atggtgtagc
     7801 accatacaaa tcagttattt ctcaaggatt tgctttagat ggtgaaggcc gtaagatgag
     7861 taaatcttta ggaaacatca ttgcaccaga aaaagtgatc aaacaatttg gtgcagatat
     7921 ccttcgctta tgggtatcta gtgtagatta tgaagcagat gttcgtgttt ctatggatat
     7981 cttaagtcaa gtggcagaag tatatcgtaa aatcagaaat acgatgcgtt tcttattagc
     8041 gaatacagaa gatttcgatc ctaaaaaaga tactgtttcg tacaatgatt tacgttctgt
     8101 agacaaatat atgaccgttc gattgaacca agtaatcaaa gaaatccgag aagaagggta
     8161 cgacaaatat aacttcatgc atatttaccg tacagttatg aacttcttga cggttgatct
     8221 atcgtcattc tacctggatt ttgctaaaga tgtagtttat attgaagcag aagatgatta
     8281 tcaacgtcgt tgcatgcaaa ctgttttcta tcaaacagct gtagctttga caaaattatt
     8341 gactcctatc attccgcata cagcagaaga aatctggtca ttcctgaaag aagaagagga
     8401 gtacgttcag ttaagcgagt ttcctggtta tgaagagttt gcaaaccaag aagaattgat
     8461 ggatacctgg actgctttca tggacttccg tgataatgtc ttaaaagcat tggaagaagc
     8521 aagaaacagc aaattgattg gtaaatcttt ggaagcaaaa ctaactgttt atccaaaaca
     8581 acaagttcgt gaaatgttaa aagctttgga tgcagatatt gcacaattat tgatcgtttc
     8641 accagactac tttgaagtta tggcagcaga tgaacaagtg ccagataacg ctatggtatt
     8701 tgatgatgtt gctattacag tggaaaaagc agacggggaa acttgcgatc gctgtcgcca
     8761 agttcgtaaa gatgtaggag tagatgaaaa acttcctcat ctatgcggtc gttgtgcaaa
     8821 aatcgttgaa gacttctatc ctgaagctgt agctgaagga ttcgaagaaa agtaaaaggg
     8881 aaaagggggt gtgacatgtt ctttgtcaca cccccttttg ttatcaatcg tttaattttc
     8941 cacgttcacg ataccagtta tctggttgcc agatccattc gaatccgtct ttttcaagta
     9001 gttcgaaagc tgctttaggt cccattgatc cagctttgta gttgggaaaa tccggttgtg
     9061 tttggtccca agcttttcga atgacatcca cgatatgcca agattgagcg acttcttccc
     9121 aatgagagaa gttcgttcca tcaccattca acgcatctaa tagtaatttt tcgtaagctt
     9181 ctgggctatt ttcaaccatt tctgcactat ttctaaattc caatttgatt ggttccgttt
     9241 caaatccttg ccctacttct ttcccattca aggataaaga aaatccttca gttggctgaa
     9301 tatagatcgt tagaacatta ggtggaagtg tcgtatcgtc acatggttca tcgacagatg
     9361 ttttgaatac attaacagga acttgtttga atacgatgtt gatccgagtc cctttttcag
     9421 tcaaacgttt gcctgtacgt acatagaaag gaacacctga ccaacggaag ttatcgatag
     9481 tgaatttgcc tgcgacgaat gtttcagtct ctgattgagc atccactttg tcttcttcgc
     9541 gatagccctt gaattgtaca ccatccaatt caccttgagc atattgcccg cgaacgaagt
     9601 tttctaacac ttcttcttcg ttataaatgc ggatagcttt caatgctttg atcttttctg
     9661 tacggatttc tttttcggaa aatgctactg gcggttccat tgcaagtaat gctacaacct
     9721 gtaaaatatg attttgcacc atgtctttaa gtgcgccact atgatcgtag tagcccccgc
     9781 gatcttctac accgaggttt tcgccaaaag taatttggat attgtcgata tagcgattgt
     9841 tccattgagc ctcaaaaata ttattggcaa aacggatagc tgagatgttt tggatcattt
     9901 cttttcctaa ataatgatca atcctgaaaa tatcttgttc tgggaatact tctctgattt
     9961 gctggttcaa ttcaaaagca gactggtaat cagaaccgaa cggtttttct atgatcaaac
    10021 gattgaagcc attttttgtc ataatatttt gtgatttcaa atgagaaacg atcgttccaa
    10081 aaaattgagg tgccatagcc aaataataga cacggttacc gtttaattga tagtgtccat
    10141 ctaatcggtc agacaattct tttaatgtct gataatgcgt tgaatcattt acatcatggg
    10201 attgatagta aaaatggctt gcaaaggtag ttgcttcttc atctgaagga ttcaatgcac
    10261 ggattgtttc tttgacaact tcacggtagt gctcatcgct ccagggacgg cgagcggttc
    10321 cgattacagc aaaatgctcg cctaaatttc cttttctgta aaggcggaac aaagacgggt
    10381 acaatttacg ctgggctagg tcacctgtac cgccgaatat cgtaaacaat acattttttt
    10441 cattagacat gtggatacac tcctttatat tttatcgaca aaaatcgtgc tggccgcctt
    10501 aaagctgacg gggattttct ttctatcatt ttggatagtg atcggtcctt cataagctgc
    10561 gatgtcaacg atctcatatt cttcgttgat attcaggtca atagtgacta agtaatccaa
    10621 caattctttt tcatcaagca ctcgtgcgat acgcactttt gtaccgacag gataggactc
    10681 caatgtttgt cggcgttctt catggacaac ttcctctttt ttaggaatcg atcctccatg
    10741 aggacaatgt tcaggatgat tcaaatagtt ttctaaatga tctgcaagat gttccgaagt
    10801 cacatgttcc aatacttcag catcatcatg gacttcattc cagctatagt ctaaatgttc
    10861 aactaaaaaa acttcccata agcgatgttt gcgaatcaga ctgctagctt tttgcagtcc
    10921 gcttgcagtc aattgtacac cttgataagg ggaatgttct acaagctctt ctttcacaag
    10981 tttagaaatc atttcactga cagatgcagc tgagacatct agactagata cgatttgttt
    11041 attgttgact ttcgttgtat ctccacctag ttctaaaatg attttcaaat aatcttctcg
    11101 atttggtgtc atattcgatc atcctttcat ggtaaaaaac catcattagt aagtgtaaca
    11161 aaaaaaacgt taattgacta tttttctaaa taaaaaacaa aggattttta gacgttttta
    11221 caacaaaaaa cgaaagatga tagagaagac tcattctttc gtttataaag gatatatata
    11281 tacattggga gggatctatt tttcagataa caacttatat gaagtgtctg cagattcgtc
    11341 ataaagagtt tgttcgggat aagaaaagat aagataaaaa ccatactgat cacgtacgac
    11401 taaacgatat tcatctattt ctaccacaga tgtgccaaga gactgaccgt tgatcaaaaa
    11461 cgtgtaatca gatgtaatct gtaattgttg tttttgctgg ctatcactaa agaaccatgt
    11521 accttgatac tttgagagtc cttctgactt ttccgcttgt tttttctttt tcaagtaaat
    11581 aaaacttgag agaacggtaa tgagtgaacc aagaaaatag atcatttttt ttatcaccaa
    11641 atcactcctt tatgagaaaa taatcgcata gacatattct ttcttcttta tttcattgta
    11701 ccgaataaaa gctaaaattt ctctaagact ttcgactggt ttttttactg tttttctaca
    11761 aattatgata ggatacatag taggaagaaa ggtgacataa tttgaagtat cagcaagtgg
    11821 aaatagcttt ttttattgac cgcccgaatc ggtttatagc acattgtatg aaaaaagacg
    11881 gagaaataat tattgcgcat gtcaaaaata caggtagagg aaaagaggtc ctgataccag
    11941 gcgctgaagt ggctattgta tttgctcctg gacctaaacg gaaaaccgcc tatgatctga
    12001 ttgctgtaaa aaagaagggt gactggttta atatcgatag tcaattgcct aatcggctgg
    12061 caattgatgg tattttagac ggcacgatcc ttttaccagg attaactggt ccatttacgg
    12121 tatacaagcg tgaagtaact tatcttcact ccaaatttga tatttattta gagacggtgg
    12181 tcggacaaaa agcatttgtt gaagtcaaag gtatgacact tgaaaataaa gcgattggtg
    12241 cttttcctga tgcgcccaca ctcagaggat tgaaacatat aggagaatta acgtatgccg
    12301 cacaagatgg ttatgccgct tatgtcttgt ttattgctca gttcgaacat cttcatctgg
    12361 caacgatcca tgaagaaatg caaccagcac tagctgatat ggttcgacat gcgcagcaaa
    12421 gcggagttca aatcctcgcc tataattgtc aagtatcggc agatgaggta actgtcaagc
    12481 aggccattcc atttgatgta gatatccctt ttgaagatcc tatacaatag aaagaaggta
    12541 aaataatgat caacttaggt attatcggaa caaattggat tactcatcaa tttgtccaag
    12601 cagctttaag cacaaaaaaa tatgaattga cagcggtata ttctagacat ttggaaaaag
    12661 cacaggaatt tggttctcat tatgaagggg atatcgaatt cgcaacagat ttgaagactt
    12721 ttttcaatat tactcatatg gacaccattt atattgcttc tccgaatagt cttcacttta
    12781 gtcaagctaa gcaagcaatt ttggcaggaa aaaatgtgat agttgaaaag ccagcttttt
    12841 cgacgccaaa agaaatggat gaaatcatcc atttggcaaa tgaacacaat gtgtattttt
    12901 ttgaagcagc aagaaatatc catgaatcag gttttaaaaa gattgccgat tacttaccat
    12961 taaaagacga gataatcggt gcaaatttca gctatatgaa atattcttcg cgctatgacc
    13021 aagtattaga tggagaagaa ccgaatatct tctcgactca tttctcaggt ggtgcattga
    13081 tggatttagg tgtttacctt gtatatgcag ctgttgcttg gtttggaatg ccaaaagaag
    13141 tccattattt cccaagaaag atttctactg gtgtagatgg tcttggatgg gggatcttac
    13201 gttatgagaa ttttgatgta tcgatccagc caggtaaaat cggtgattct tatttaccgt
    13261 cagagattta ttttgactca ggaacgctag tgatgaatgg agtcaatgca attgaacaag
    13321 cacaatttca tgatcgcgcg cataaagaaa tcattgattt ggcggtaaca gctgcagaaa
    13381 atccaatggc agatgaagca gaggattttg ccaatgtcat ggctcatccg aaagatccag
    13441 cttggggatt gttgtatgaa gagtggatcg agctagctag aaatgtgaac caagtgattt
    13501 atgatttaag aaaaaatggc ggcatcaaat tcgatgcaga taatgaggaa gatttttaat
    13561 gaacgaattt gtacaagtat ttcccgaaaa ttggcagaaa aaatatgagg ataaagggtt
    13621 tatctcacct tctcttattc aacgagcagt ttttcagcca ctaagtaata aacaaagtat
    13681 agtagcggtt tctcctacag gttcaggtaa aacactggct tatctttggc ctctactact
    13741 aaacgtggaa ccaggtgaag caagtgcgtt ggtcattttt gcttcttctc aagaactagc
    13801 tattcaagta gcagatgttg cacgtgaatg gggaaaagac aaagagttga aagtccaatc
    13861 tttggtgggc ggagccaacg tcaaacgtca gattgagggt ttgaaggaaa aaccagaaat
    13921 cttgattggt acaccaggaa gaatattgga attgatgaaa gcaaaaaaaa taaaagcaca
    13981 tcaagtgaaa acaatggtat ttgatgaagc agaccagcta tttgacgctg ggaacagcca
    14041 aatcattgat caaattcttc atcaagctcc aacagagtat cagctagctt ttttttcagc
    14101 gacagcagat cgttcccttg aaagcattga aaaaatcact gggaaaacgt ttgagacaat
    14161 tgacgtgaca gcagaggacg attctcgaaa aggactgaga cattactttt tacgtgtacc
    14221 gatacgaaag aaagatgact acttgagacg tctcacgcat atagaagatt ttcatggtct
    14281 gattttcttt aatcaactga ctgaattagg cgtaatggaa gaaaaattgc tgtatagagg
    14341 tgtaccggta ggaagtctgg cctctgatca gaacaagcta ctccgaaaag cagcgctaga
    14401 acaattcaaa aagaaaatgg tcagagcact tttgaccaca gatgtggcgg ctagaggttt
    14461 agatattaca ggtcttcctt atgtagtgaa tgcagaagtt ccgttaagtg aagaagctta
    14521 tttgcatcgt tcgggaagaa ctggtcgaat gggtaatgaa ggaatagtaa ttacactcgt
    14581 acaagataat aatctaagag atttaaaacg gcttttacgt ccatcaaata taacacttga
    14641 agagattttt ttatacggag gtaagctgca aactgagcag ccaaccaaag aagatgcagc
    14701 tcctgcagta aataaaaagt atgcgaaaac tttttctgga aaggagcaga acccagaata
    14761 taacaaagaa aaaataagaa aaaaagttaa aaataaatca aaaaaagaga gaaataaagg
    14821 agcaaggcgc aaataagcgc ctttttatgc tatgattatg aaaacgggaa caaaattggt
    14881 tgccagagga ggggttatgt gaaagaacgt caagaagaga tcttgcagct attgacagaa
    14941 aaccaacaag gattgacagc aagtgatgta gctgagcgct taactatcga tcgaagcaat
    15001 gcaagccgat atttaagtga actataccag gcccatcata ttgtaaaaac cgctggccga
    15061 ccagtcgttt actctttgcc aacggaaaag tcaaaatctg atgaagtgca tgtcgactct
    15121 tctacgcaag tgacttttga aacactcgtt ggagaaaatg attctttaaa agtcagcata
    15181 cagcaggcaa aagctgcgat tctttatcca ccgcgggggt tgcatacgat tatttttgga
    15241 gaaactggta cagggaagtc gatgtttgct gaatgtatgt atcattttgc aatcgattca
    15301 gagatgcttt ctgcagatgc gccatttgtt tctttcaatt gtgcggatta tgctcaaaat
    15361 ccgcaactgc tctttggaca catctttggg atcaaaaaag gggcttacac aggagcagct
    15421 caagatagtc ccggtctaat agccaaagca gacggcggta tattgttttt agatgagatc
    15481 caccgtttac cgccggaagg gcaggaaatg ctgtttactt tcattgataa aggtatttac
    15541 cgtccgcttg gagaaagtgc acaagtacat gaagcatctg ttcagatcat tggggcgacg
    15601 actgaatctt cagaaagttt cttatctacg ttcaatcgac ggattcctat ggcaatcacc
    15661 ttgccgaatc ttgcttcacg ttcactggat gagcgttatg aaatcatctc tctttttatc
    15721 aaacaagaag cgaatcgttt gaatcaacgt atcgatatcg aaaaagaagc gattcttgcc
    15781 ttcatgcttt atgatgcgga aggaaatatc ggtcaagtga aacgagactt gaaactagtc
    15841 tgtgcaaaat cttttttaca ttaccgtact catcatgaga aaaagctaat cattcgaaaa
    15901 gaagaacttt cccttcaagt acaaaaagga ttactgaaaa tcaaggaaat accggaacgt
    15961 ctcgaccgat tcatggacag caaaagtcag tatttgacat ttgaaccagg atttgctgat
    16021 gttgtctggt cacaagatcc agaacgaaac atgcaggtct ataacgatat tgaggaaaaa
    16081 gtcacttctc tttccgaaac cggtgtggag aacattgatc tagagaccct catttcaaaa
    16141 gatgtggatg cttatttcca gacatatgtc aaagaattga ctaaaagttc gattcccaaa
    16201 gatcttttgc cagatgaaat ctggcggtta accaatcaat tgtatgatgt ggctgaaaaa
    16261 cggttagacc gtatttataa tgaaaaagct cgttttgctt ttgctttgca tctgcaaagt
    16321 acattggatc gtgtgagaga aggtcatatg atcgtccacc cagatttgaa taacgtacgg
    16381 aaaaatttta agagggaatt ccaggtagca atcgatcttt caacgatcat tgaagaagaa
    16441 gaacaagttg agattccttt tgatgaaatc ggctttatta gtatgtttct ttctatcaat
    16501 ctaggagaaa gtgagcctgt taaagaaaac aaagtagatg ttctcatatt gatgcacggc
    16561 agacagactg cttcaagcat gttggagatg gcacaagaac tattaggaac aaaagtaggg
    16621 aaagctttta atatgccact ggttatggaa gttcaaacaa tgtatgaaga agtgttacaa
    16681 tatgtcaaaa actatcaaga gcatttgacg aatggcctac tcttgttaac agatatggga
    16741 tcattaaaca ccttcagtga tttgatttat gaagagactg gcattcggac gaaagctatc
    16801 acgatgacaa gtaccatgat tgtgatagaa gcgatacgga tggcaagtgt tggaagaagc
    16861 ttagaagata tttatcaaaa tatccagctt tcctttgaat cgatcgtacg tgatcagttc
    16921 aaagcggaaa agtctgaaac tttacagcgt aaaaaagccg taatcgtgac atgctttact
    16981 ggtgaaggag tagctgccaa attatatcag cgtattatgc cagtgattaa tcaaaataag
    17041 gtagaactga tccagatgca gtttttagaa cgtgaatcat tcaaaaaaca catcgatgcc
    17101 ttattggaag aatatgagat caaagcaatt gcgggaacag ttgatgttga ttatcaaaat
    17161 atcccatttt tttcagccta tgacgttttt gatgatgaaa aactaaatat tttaaagcgt
    17221 atagctgatg atgagatccc gacagctaaa attgtcgatt cacttgctgg atcattaagc
    17281 gatgtctttt ctattgatga actgatccgt tcattgcaaa agacagttca gcagatccag
    17341 accgatttgt cgattatcgt ggaacctgga gtagatactg gaatcgttat ccatttagcc
    17401 ttcatgttag acggattgat caaacaagaa agccatcggc aatttgagaa actgtctatt
    17461 ttctcaaaaa tttatcgttt ggaaatggat atcgttcggg caaacttgat ggcactagaa
    17521 cgaaaatatc aggtaaaaat tccagaagat gatatcgcat ttcttacaca aatgtttctt
    17581 gaaaataaaa taaaaaaatg aacacactaa tcatactgtt tacacactaa acatctgtgt
    17641 gcgcactata aaaaagagct aaaaagcatg aaaacgctat ttcagctctt ttttttattg
    17701 gcatgcttct tgcgttatat aaaagtgtaa aaaaataaaa ggagtgttaa aaatggcaaa
    17761 aaaaacaatc atgttagttt gttcagcagg gatgagtacg agtttattgg tcacaaaaat
    17821 gcaaaaagca gcagaagaaa aagggatgga agccgatatt tttgcggtct ctgcctcaga
    17881 tgcagataat aatttagaat ctaagaatgt cgatgtatta ttattaggtc ctcaagtacg
    17941 tttcatgaaa gctcaatttg aacaaaaact tgcgcctaaa ggtattccat tagatgtcat
    18001 caatatgcaa gactatggca tgatgaacgg agaaaaagtt cttgcacaag cagaaaatct
    18061 aatgaaataa atcaagtatg cagttggggg tataacaacg tggaagatca acaaaatcta
    18121 gaagccatca tggggttaat catgtttggc ggtaatgcaa agagcgatgc aatggaagcg
    18181 attgcggctg ctaaaatcgg tgattttgag ttagcggata ggaaaatcgc tgatgcagaa
    18241 gaatcactta tacaagcgca tcatgcacaa acagacatgt tgacacaaga agcacaagga
    18301 aatcatatgc aagtgacatt attgactgtt cacagtcaag atcacttaat gacatctatt
    18361 gcgtttactg atttagcaaa agaaatcatc gaactttatc gtcgcgtata cagcgaataa
    18421 atacaacttc ttttttgaac ggaagaagaa aaatttttga acggattttt tattttgtta
    18481 taaggagaac tctctttata taaaaaactt ataggaggaa gtaaaaaatg aacggactat
    18541 caagttggat ggagaaatac atcctgccgg ttgccgcaaa aatcggagcg caaaaacatt
    18601 tagttgcttt gcgtgatgct tttatcggca cactgcccgc aacaatggcg ggatcagtcg
    18661 ctgtaatgat caatgcaatt attcgtgatt taccgcaaca gtttaacagt acctatgctg
    18721 acagtttagc agcagacaaa ggaatctttg ccgtattgaa tgtcatcatc ggaatcaatg
    18781 gttttgtctg gaacggtaca ctagcaatcg ccggattgat ctttgccttt tcttgggggt
    18841 ataatcttgc ccgagcttat ggagtaaatg atctagctgg ggggatcgtt ggtttagcta
    18901 ccttgattca aggtatttcc tttggtccag cattatcagc agcattagat gtgaatgttc
    18961 cttcaaatgt cgcagatgca atcaatagtt ctgagattgg tgcaacgatt gcagatggca
    19021 cactgtcagt tggattatgg ggttggctaa aactagatca tttgaatgga aacgcttatt
    19081 ttacagttat gatcatggga gctatttcag tcattatttt ttgtaaattg atgcttgcca
    19141 acattacgat caaaatgcct gattcagtac caccagcagt atctaaagca tttgcagcca
    19201 ttttaccagc gacgatcgct ttatacgtca ttgctattat caattatatc gtaggtcgtg
    19261 tagctgatgg gcaactgatc attgatttgg tgcaaaaata tattgcagaa ccattcctag
    19321 gattatctca agggttggga gcagtattga tcgtcacagt atttgttcaa gtattctggt
    19381 tctttggtat tcatggacca aatgtattgg caccagtgct agagggaatc tggggacaag
    19441 cacaactaat caatattgat attttccaaa aaggttataa cggaaaaact ggtacaccag
    19501 ccgtgttaga tgcaatcgaa gatggaaaag cttatatgtg ggtccgcgga tcgttcgatg
    19561 cttttgcatg gtttggcgga tcaggcggaa cgatcgtctt actgatagct atcttgattt
    19621 tctctaaacg tgcagattat ctaacagtag ctaaattatc cattgggcct ggtatcttca
    19681 atatcaacga accagtgatg tttggtctgc caatcgtatt gaatgcaatc atgtttatcc
    19741 cattcttgat tgctccagta gtagcaacag caattggatg gacagctact tatcttggcc
    19801 tagtcgctcc ggtatcgcag caagtcacat gggtagtacc gccgttctta ttgtcattct
    19861 tagcaacagg agcggattgg cgggcaccga tcgtaacatt agtatgtatg gtcgtgactt
    19921 tcttgatttg ggcaccattt gtcatctctg caaacaaaat ggaccctgcc gcaggcgaac
    19981 aacaataaga tgaacgtgag gagcgggacg ctttaagttt cgttcctcat aatgaatttt
    20041 cgaggaggaa ctgaaatgat tacaattgcc agtgcaatgc aacaagatga aatcaaagaa
    20101 ttgttagaag gatacaatga agaaggaatg acttttacat ttaaagaaaa acaaggaatc
    20161 aagctgttct ttgaaacaag tgcagaagat ccggaagttg cggctaaaaa agcaaaggaa
    20221 ctaatcaaag cacagccttg gggaacagtc ctttattttc aatcaacagc tgtttagaaa
    20281 atggtttaaa aagataggat ggttgagatg ataaaaaacg gtttattttt atgtagtatt
    20341 ggttctttga tcgtgttgta tagtttgaat ccacattcgc aaaggtatga tttgtatagt
    20401 atggtcacag gtctttttct aacaggatta ggaatctatt tcttcatgaa ggggaaaaaa
    20461 agagaagaag caaaggagaa aaataactaa tggaaatcac gaaaaaatta aatattccag
    20521 catcgttttt ttacgataaa gttatggact ctgtcctttt cgatgtacgt aaagcgaccg
    20581 gcaaatcagt aactagaaaa cagctgaaaa atttggagta cgtcaaacaa ttctcgcaaa
    20641 ataaccgggc tcgcataaaa atcgaagaag tcattgaaaa ccagtcttat aagtttcgta
    20701 catcgacaac gaagaatgaa ttcgtcgttc attatcaaat caaaccattg gacgattcga
    20761 cctgtgaggt tcattacaca gaaaaaatgg agtcttatgg catgcttcaa aagttgaatg
    20821 atatgctttt aggaacaatc ttgatgtatt ttaaacggag acgtttcaaa aaaatgctag
    20881 atatgatcga agcaacgtat taaatcaatg aaacatggta ctatttagtt attgaaaaat
    20941 gaagtaagct agaaggaatt tttcatagga agatagatct ttcctgcgaa tcctcactag
    21001 cttttttgta ggaaggggca gcaaaatgac aaacaaagta agagagctag gtgtatcttc
    21061 gattgaggaa atgtttgatt tagccactta tgcatttaat ggggcagata cacctgaaag
    21121 gcgagaacgt tttcgtacac ttgcggagaa ttcttggaat tacggatttt ttgatgagga
    21181 ggatcggcta acaagtcaag tcatggcgac cccttttcca gtgaattttt acggacaaga
    21241 gtacctaatg gccggcattg gctatgttgc ttcttatcca gaagcaaggg gacaaggcgg
    21301 gatcaatcag atcatggaaa agatccttga agattgccgt gatcgtaaag taagcttatc
    21361 ttatctggcg ccattctcct atcccttcta tcggcgctat ggctatgaac aatcatttga
    21421 taaaatcagt tatcaactaa acagtcgtga tgtgccgttt atcaaaaaaa aatctggaaa
    21481 aataaagcgt atggattttg aagatgcaaa agctgcaatc agaagaattt atgaacagat
    21541 gccggaaaac caacgagctg gtctgaagcg cgaagactgg tggtatacgt ataaattcaa
    21601 gcaaaaaggc aattaccagt ttgctttgca ttttgatgat aatgaggaag caacgggata
    21661 tatcgtttat cagcttgcaa cgtcttcctt tattattgcg gagtggggat ttttggatca
    21721 tgatgctttt tgcagccttg ttcgcttcgt aagttctcac aatggtgctt ttgaaacatt
    21781 tgaatattca tgcggctacg gcggtaatga tcaaaattat ctattagaaa atccatttgc
    21841 ttctgtaatg atcacacctt atatgatggc gaggatcgta gatatttctt tgtttttgga
    21901 aaattatcca tttaaaaaga gacaagctgc atttgcccta caaatagaag aagatcatta
    21961 tgcgaaatgg aatgaaggaa tcgtggaagt aacaatcgaa aatggaaaaa atcatctctc
    22021 aaaagtagaa caaacaaaac tcccaatcgt tcgaggaagt atccaacata tcacgcagct
    22081 attattaggg tatcgtaaag tggaagagtt ggtattccaa gaaagaatac aggtgacaaa
    22141 agaatatgaa gaaacgttaa gctcaatctt gccacagcaa cagccaattc ttaatgacta
    22201 tttttgataa aaaacgggtg atgacaatat ttttgtcacc acccttttgt ttttcatttg
    22261 gcatttgaag gaaaatgtta aagtaaatcc tcttttttcg taaatttgaa ttttttgttt
    22321 tcttttgttt tagggttttc ttttttgtgg tcttggattg cttctttcgt aatcttttcc
    22381 cgattttttt tagatgaatc taagcgttct gcgtatactt tttcttttgg cttatcattc
    22441 attggaaagg acctccctac attatttcat tctacctcta gtttatcatc tgttaaggac
    22501 tagtggaaac gttttgccta acttataagc tgattttttt agcagataag cttgcaagta
    22561 aaaaacaaac aagctacaat tgatttattg aaattgttcc tgtagctcag taggatagag
    22621 caatcgcctc ctaagcgata ggtcgctggt tcaattccag tcaggaacgt ataaataacc
    22681 cctgtatcct ttgcggtaca ggggtttgtt ttgtatttgt gccaaaaatg tgccaaacta
    22741 gttatcattt ttatataaac tatcgaacac agtattgatt ttttctttat cctgatcttc
    22801 aagctctcga acaatatgaa gataagtaga cattgtagtt tctaaactgc tatgtcctaa
    22861 acgtttcgat acgctaagta tatttactcc ttgataaagt aaaatagatg catgtgtatg
    22921 ccttagtcca tgcaaagtaa attgcttttc aatccctaat tcttttaatt ttttgcgcaa
    22981 atatttgctg acggcgtttg atgaaactaa tccatttttt aagttgaaaa aaacaaaatt
    23041 atgagggttt ttgatttcaa aattctcgaa taattctttt tgattgattt taaacttttt
    23101 taagagatca atcgtatgtt tatcaatgga aattttccta tttgaagttt cgttttttgt
    23161 ttttccccat tcatttaatt tataattcca tgttttatta atagtgatga tttgatcttc
    23221 gaaatcgata tcttcccatg ttaatcctag aagttcagca aatcgcattc cagtggcacc
    23281 agcaactaac acgagcatag gagaagagta ctgcgcagaa aggttttctt ctacgagttt
    23341 cattaaagat ttaaattgat cataatctaa atatttatct tcttttggct tcaatgaatc
    23401 ttttcctttg attactgctt ttctagttgg atcaaacgga attaatcctt cttctacggc
    23461 atctttcaac gacgctctaa tatggttatt aaacttaata acggtagatt ttacatggtc
    23521 tttagcatac ttatttaaaa agcgttgata tcccactcta tctaaatcag aaatcaatac
    23581 cgctggcata tatttcttta tgttcattaa cgtatcttcg tattttctat aagtgatagg
    23641 agatactgtt ccttctttat aaagttgcat ccaatcctca aagtaatctg ataataatag
    23701 atttttgcga tcccctttaa ggcctttgtt caagttatat tctaattcgt tggcggcatc
    23761 ttttgcttct ccttttgtct taaatcctga ttttcttatc ttcgtgtatt ttccgtcatc
    23821 ttttttgtat gaaatttcgt attgccaact atttccacgt ttgactaatc ttgccataat
    23881 tgaatcagct ctctttctct gatacaatag gaactataaa gaagcccatt gtttgggttt
    23941 tattattttt tgcacatcct ttttgcttgc cggcgcaagg atgtgctatt tgtcgtttta
    24001 atggtataat tggatgtgta ttagatcatc caatcagagg tgtttattat gattaaagtg
    24061 atttttaaaa gaataactga acttccagag tttagattat ttgttgatta ttggtatgtc
    24121 tggtttttta ttgttgcatt gctgataggt ctagatgtca tattagaaag aaaacttaag
    24181 taagagttga gttaataaat tgaattccta aatgagatat tttgtagccc atattttgtt
    24241 tgccgacatc acctaatact atggagctaa tcgttttttc taacagacca aaatcagata
    24301 atctatttaa gctaccatct attagctctt gtgaatagcc agacatgctg atatttccag
    24361 tgaaattttc gtttccacga ctcattaagc atgctaacaa ttgaatctca atcgatgtta
    24421 attgattcag tacaagaaga taatactcct cgttatccca tgaagcgttg ttaattcgaa
    24481 gtaaacaatt cttataaaga tttttataca tatctatttt attttgtgcc tttgattttt
    24541 ctacctcgtc atttatccct tcgaatattc caatgagttg gtccttgttc tctaagttga
    24601 aaacatcttc tggaatttga tcgcgaatgt tttctaatga ttcgtttaaa tttttgtaga
    24661 atttctcgat gcgtttgaaa cgcttctcgt ttttagaccc aaaataaagt gactgcacaa
    24721 cgccaccaat ataaggaatt gctccaatac cgccttcaac tattaattca agatattctt
    24781 ttttggttag tttttcattg ctattatcca tagttacctc ctattttgtc atttccttta
    24841 caagcattgt gactcctgat ggcactcttt tgtataggtt ttattattgt gcacaatcta
    24901 actcttagtg gggcgtggtt catttatttt ttacttactt tactattaat catatcccat
    24961 tgagcatagg atttttctat gttcgatcct cttgaactaa tttctcctgt aaaagttact
    25021 ttatcgccct ttgtaaaggt atcagcattg tttaagtcct tcgcaaaaat tacatatgaa
    25081 actttaggtg ttgcagaaat atcattccaa cttttatcat cgtactcttt gtctgctata
    25141 atggctattc tagtagtcat tgattcgata acagttcctt caaaagtata tgttttacca
    25201 ctaatatatt tagtaaaagc tgtattttgc tcagtagctg gagtaataga ataataacta
    25261 tctaagaaag caccccagtc atgattattt gtctctaaaa attcttcaaa ttgagttagg
    25321 cttttattgc tgctgttaga tgtagtagtt tgagtatcag atgatgagct ttcctgtttg
    25381 ttattgtcat ttgcctcgtt tgatgaggaa agcgagaaac ctacggtagt aaaaataatc
    25441 ccgataataa ggagaatcat aggtgttttc ttatttgttt ttttgataaa tcttacaatt
    25501 aac
//
LOCUS       DAZKLD010000025        25416 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.025, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000025 DAZKLD010000000
VERSION     DAZKLD010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25416
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.025"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            141..2201
                     /locus_tag="ACG8KR_000862"
     CDS             141..2201
                     /locus_tag="ACG8KR_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterial Ig-like domain-containing protein"
                     /protein_id="HFQ0200257.1"
                     /translation="MCSKRRSKQLNKHACRLDKERSCSEKHYVKFMMRTMLISLSVGV
                     YMHVPVQATTQTIKVVRTSVSETTDSPITITHTFTDANGKQLLPALTSYNKVSYPPTI
                     NHYWLIPNKTLWTSETGSSVPLSIFSDMTDPTKGDQLKQITDILNHALTDIKDGGSAQ
                     VSYVYEKDYSQFERKKIDVLAGTKVSISDLVERLKNADGTQGDMSKVISNNGNIVDTT
                     NTGTSTISLSYFDPIALKTMTASAVIHVRADQTAIKGKDLHLRTGESYAPKDLVEYVT
                     KSDGTSGNKEEIKMNGTVDTTTPGEYPVVLTYIDSKTSNVAETTAVVYVEASAASSTV
                     ITHFIDSQTNKELQAETHMSSVNRIPDLRLPAEFSDRVPNIDLSTYEQNGKVTSISDW
                     MQANGLDRKSHWSEILEVLHSELMNNRKQSGGEITFTYVYIPDNRSLEGHDLIVHPNE
                     KINKEELIQSATNSLGKPVPLKDVSVTINEVPLEDGYLTKQTGDFTIMYSYHDPYSLQ
                     ELHAQSLLHVLPLNKQIDQKEQKRQEESLSEKKQQMAHAVSPTGKVKQTRSLELARIS
                     RKISESNKTIRQEAHLAPQVSGLENEILEPKIKQQSEKEFVAKQSEDQKESDDQKETK
                     KKRLSTIDSTDEHLQPQVPLPSPGTPAPGGGIPSSALGDFMRGMAGTWVYANRNEFDA
                     DDWF"
     gene            2284..2853
                     /locus_tag="ACG8KR_000863"
     CDS             2284..2853
                     /locus_tag="ACG8KR_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0200258.1"
                     /translation="MLDKTLPYAEIWMTRPLQQNVPITPLAEGFEFRFYQKEDESAWA
                     EIETSVGEFQTTDEALEYFQQNFAPYPEELERRMFFVETKKGEKVATCTAWRKKSREQ
                     VYPVFHWLAVKPDYQGKGLAKALTARVLQEFPTLHTDGPIYLHTQTWSHQAIALYKKM
                     GFTFIAENLDGTPNPDYEKVMQVLSKLDK"
     gene            complement(3028..3834)
                     /locus_tag="ACG8KR_000864"
     CDS             complement(3028..3834)
                     /locus_tag="ACG8KR_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0200259.1"
                     /translation="MNHLKTFFTNSTKTAGRITNGIYILLRIIVVIMLVDQVWNQHWG
                     NTMLLLLTLILFEIPYFLEKILKIQIPNMLEIILLSFIFSSTVLGELSDFYGYFSMWD
                     TFLHAFSGFLAGGVGFSLVYLLNKNTKTMNLTPLLLAVVSFCFSMTVGVMWEFVEFSA
                     DKLLQFDMQKDTIIQQINSVELSKDGNTVYHVPDITRTIIESQNADGKTDKTIIQNGY
                     LDVGIMDTMKDLFVNFIGAVVFSVFGYLYSRNDYQKFTFIKNFIPKKIKR"
     gene            3972..5486
                     /locus_tag="ACG8KR_000865"
     CDS             3972..5486
                     /locus_tag="ACG8KR_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HFQ0200260.1"
                     /translation="MKKLVAGLLTAVALLAMSACGADQKNTAQTSSSTKESAEVSSGA
                     SEQVYTDPKEMKESYDVVIIGAGGAGMAAAIEAKDAGLNPVILEKMPVAGGNTSKSSS
                     GMNASETKFQKEEGIKDSNDAFYEETLKGGGGTNDKEMLRYFVDHSADAVDWLDQNGI
                     KLDNLTITGGMSEKRTHRPSDGSAIGGYLVEGLLNNVHEREIPIFVNADVTDIKKNGE
                     TVNEVTVHVQGEEPKEVTGKAIVVTTGGFGASKEFIEKYRPDLADYITTNQEGSTGDG
                     ITMIEKLGGQTVDMDKIQIHPTVQQDEGVLIGEVVRGEGAILVDQEGKRFVNEMDTRD
                     KVSAAITALPEKSAYLIFDQGVRDRATAIEFYDQKGYVTTGKTMEELAEKIDLPKETL
                     AKTVDGWNTDVAEKKDAQFNRATAMEHDLSSPNYYAIKIAPGIHYTMGGVKINTNTEV
                     LDKENQPIRGLYAAGEVTGGLHGNNRIGGNSVAEIIIFGRQAGIQAARFASENE"
     gene            complement(5889..6872)
                     /locus_tag="ACG8KR_000866"
     CDS             complement(5889..6872)
                     /locus_tag="ACG8KR_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HFQ0200261.1"
                     /translation="MRAISIKHPGSVDVLEQIEVPVPKPKQGELLIKVHTAAVNRTDI
                     MKRESVSLKPPYPILGVEVAGEVVENKSDISTFTPGTKVYGLVNLGGYAEYAVMPADR
                     AILLPDTLDYVSAAGISEVFLTAYQTLYWLGKLQEGETVLIHAGASGVGTAAIQLARQ
                     LSHAKIIVTAGSEEKLAFCKELGADELINYKKQDFQEEVRKITEGRGVDVILDFIGAS
                     YWEKNLASIAVDGRWVLIGMLGGTIVPEIDLGVLIGKRVQLLGTLLTPRSDQYKAELA
                     QTFMQKAGPFLENGKIRPIIDRTFQFSAVKQAHEYMEANKNIGKIILKITE"
     gene            complement(6877..7524)
                     /locus_tag="ACG8KR_000867"
     CDS             complement(6877..7524)
                     /locus_tag="ACG8KR_000867"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding oxidoreductase"
                     /protein_id="HFQ0200262.1"
                     /translation="MKIFVVGANGQIGRHLIKDLAPSSHEIFAGVRDVASQTLVKKEN
                     VSYVSFDLTWSVEKMTEAFKGIDVLIFAAGSQGKNLLQVDLDGAIKTVIAAENAHVSR
                     YLMVSAVYADEPAKWPESMTDYYITKHYADEWLKRTNLDFVILQPVTLTNDEEVTSIQ
                     LTKPNEKASKTITRSTVAAVLAALVEETDISRTTLVLSEGSKELNTAFQEWAKEE"
     gene            complement(7609..8241)
                     /locus_tag="ACG8KR_000868"
     CDS             complement(7609..8241)
                     /locus_tag="ACG8KR_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFQ0200263.1"
                     /translation="MKNTKNFTLTAMFLAILILLAVTPLGFIPIGPINATTMHIPVII
                     ASIVLGPRLGAFLGGTFGLISMIRSTVIQTPLSFVFSPFIPVIGTEHGSWKALLIAFI
                     PRILIGVVPYFVYKGLRKLMKQKADSVSLFLAGLSGSLTNTILVMNMIYFLFQQDYAK
                     VIGTNLNAVYSAILAVIFTSGIPEGIVAGLATAAVCNVLLRYFKHPSLTN"
     gene            complement(8257..8808)
                     /gene="coaC"
                     /locus_tag="ACG8KR_000869"
     CDS             complement(8257..8808)
                     /gene="coaC"
                     /locus_tag="ACG8KR_000869"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HFQ0200264.1"
                     /translation="MKKILLGVTGSISAYKSADLTSQFVKLGAQVDVIMSKSSTHFIT
                     PLTLQSLSKRAVHTDVMQESNPQVINHIELAKQADLFLIAPATANIIGKLANGIADDL
                     LSTVAMALPVGTPKLIAPAMNTNMYQNPITQRNLETLRSVGYQEIEPRESLLACGDFG
                     KGALADIQTIIEQVMLTLNERNA"
     gene            complement(8805..9542)
                     /locus_tag="ACG8KR_000870"
     CDS             complement(8805..9542)
                     /locus_tag="ACG8KR_000870"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HFQ0200265.1"
                     /translation="MKILITAGGTTEKIDQVRAITNHSTGRLGQALADYLAANPDTTI
                     DYVTTKQALKPKRHSNITIYTIESALDLFLQLEELTKKEHYDAIIHSMAVSDFTPAFS
                     FSEEQLAKNLPTSSTQEELDNWFAENEQTDTTVSKISSNTEHLVLVLKKTPKIISYLR
                     EWQPKAKIIGFKLLVDVPKESLLAVAKNSLINNKTDFIFANDLTEIHGETHHGYLLSK
                     DGTVEEAQSKSEIAALITEKIRLEESK"
     gene            complement(9539..10528)
                     /locus_tag="ACG8KR_000871"
     CDS             complement(9539..10528)
                     /locus_tag="ACG8KR_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFQ0200266.1"
                     /translation="MNAFRSFQVIKEPVFHTAISDLPFTELAENEVLVKIAYSDVNYK
                     DALAMSESGQVIRTYPMTPGIDLSGTVVQSNNPRFSKEDLVLATGFGLGVTHPGGYSQ
                     YQKVPGDWLVPLPKNMTLRQAMILGTAGFTAMLCVNALIRQGMTSDKKVVVTGASGGV
                     GSTAIALLHKLGFQSIIAFSRKEESVTWLKSLGALQVVRPDEFLPETTKALGKQQIDY
                     VIDTVGGEQLSSLLPLISYNGAVALCGNAGGIKLNATVLPFILRNIQLIGIDSVNVPI
                     DQRLSLWQQMADLQIADELVVQEITLDQLPETASKLLAGTHQGRTLVNVGDHK"
     gene            10786..11328
                     /locus_tag="ACG8KR_000872"
     CDS             10786..11328
                     /locus_tag="ACG8KR_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HFQ0200267.1"
                     /translation="MEIGEKLRNLRIQKNLTQEELGERTDLSKGYISQLERDLSSPSM
                     ETFFSILEVLGVTPEEFFHQEAANLQVVYSKEDHTVYFDEENGYELEWLVADSNEKEM
                     EPVLLTFEGAGEYKTFEPSLSETFIFVLEGELELSLGETVYVAKAGQSIYYQATKQHQ
                     LRNHLKKRTKVLIVATQSYL"
     gene            11345..12430
                     /locus_tag="ACG8KR_000873"
     CDS             11345..12430
                     /locus_tag="ACG8KR_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0200268.1"
                     /translation="MGKPIISFDQVVKRYDDETVLKKVSFEIEQGKFYTLLGPSGCGK
                     TTILRIIAGFTEATEGDIYFEGKRLNDLPANKRQVNTVFQDYALFPHMNVFDNVAFGL
                     KIKKMSKSDIEKKVKDALRMVQLPGYEKREISEMSGGQRQRVAIARAIVNEPKVLLLD
                     EPLSALDLKLRTDMQYELRDLQQRLGITFIFVTHDQEEALAMSDEIFVMNKGKIVQSG
                     TPVDIYDEPINHFVADFVGESNIVDGVMIEDNLVEFVGKQFECVDGGMRLNEPVEVVL
                     RPEDLTITTPDKGKLVVTVDTQLFRGVHYEIICYDEQQNEWMVHSTKKAKEGSKVGLA
                     FEPEDIHVMRFNESEEEFDARLDSYEE"
     gene            12442..13248
                     /locus_tag="ACG8KR_000874"
     CDS             12442..13248
                     /locus_tag="ACG8KR_000874"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0200269.1"
                     /translation="MKTMRRIYSVPYILWLGLFVIAPVLMIIYQSFFDMNGQFTLNNY
                     LSYFTSGTSLSMTINSVWYAFLITFFTLIISYPTAYFLTKLKHKQLWLMLIILPTWVN
                     LLLKAYAFIGIFSVHGSVNHFFDFIGIGPKQILFTDFSFLFVATYIEIPFMIMPIFNA
                     LEEMNPSLISASRDLGANSFETFRRVIFPLSLNGVKSGVQAVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLVTQNWGMGSTIGVILIIAMFIVMLLTGEKKKGGRS"
     gene            13249..14076
                     /locus_tag="ACG8KR_000875"
     CDS             13249..14076
                     /locus_tag="ACG8KR_000875"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0200270.1"
                     /translation="MKKFKWSYLYLGIVFLFLYAPIFYLIFYSFNEGGTMNSFTGFTW
                     ENYEAVFEDTRLIIIVLNTFMLAFLSALIATIIGTFGAMGIYYTKKRRARTALLSMNN
                     ILLVSPDVIIGASFLIFFTLLGFGLGFQSVLLSHIAFSIPIVVLMVLPKLQEMNDSMV
                     DAARDLGANNFQVVKNIILPYLSPGIIAGYFMAFTYSLDDFAVTFFVTGNGFTTLSVE
                     IYSRARQGISLEINALSALVFLFSMILVIGYYFISKENRPKRLKKNRRVREGVARLR"
     gene            14073..15146
                     /locus_tag="ACG8KR_000876"
     CDS             14073..15146
                     /locus_tag="ACG8KR_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFQ0200271.1"
                     /translation="MKKLQSLFLGILIIIAVLLLGVRQLEKTSGMSGAKVLTIYNWGD
                     YIDPSLITKFEKEYGYKVNYETFDSNEAMFTKIQQGGTNYDIAIPSEYMIQKMIKEKL
                     VLPLDHSKIKGLNNIDPRFLDLDFDPDNQYSIPYFWGTLGIVYNDKFFDANQIKHWDD
                     LWKPELKDSLMLIDGAREVMGLSLNSLGYSLNSKNMAQLTEAANKLNHLTPNVKAIVA
                     DEIKMYMINEEASVAVTFSGEAADMMWENEHLHYVIPPEGSNLWFDNIVMPKTVKNKE
                     GAYDFINFMLEPENAAQNAEYIGYSTPNEEAKALLPKDISSDEQFYPSDDTISHLEVY
                     KDLGSKYLGIYNDLFLEFKMYRR"
     gene            15574..18270
                     /locus_tag="ACG8KR_000877"
     CDS             15574..18270
                     /locus_tag="ACG8KR_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFQ0200272.1"
                     /translation="MEYKYRIDILQPIVYYAFRHIKLEEKMKKSAKDRVFDELIRLTG
                     NMSSVTAEKISKNLGISRQNASHYLTRLVEDKKVEKLPGKPVLWKPLDEYAVVDNTEQ
                     INEAFHSVVGHDGSLREVIQKCIAAVKYPPNGLSVLINGATGVGKSFLATKIFEYAIH
                     EQIIEKDAPFAILNCADYADNPELLSATLFGYKKGAFTGAEKDTDGLLATANNGYLFL
                     DEVHRLSKENQEKLFLFIDTGNYRPVGENVNWHSAKVRFIFATTEKGENYLLDTFDRR
                     IQISVMLPTFEDRPIRERLELIQLFFQNEADVLQKDIIVSREALSLMLSHPFSGNIGK
                     LKNIIKISCADVYSRTQEEPLTIGKNEILIQLNMLESEVESLPIASLLIKKNQKIKTD
                     RIKPFDYDSILEKITQLINEGNFNENFSSIKNEVQHLVHHIDKDSFLSMSRKLMIQMF
                     RKVWVSVLGKTYGLTTSGPVAEIISRSYVISPSYKTDLTGINRTLAANLPRTTYLCNQ
                     FLEHLPPLSEKDENFLRLLIVVTLSDYVDESIELKGLLLAHGDSTASSIQAVVNQLCG
                     NYVFEALDMPVESNVSEIVESTRNFVKRQVNTDSLVLIVDMGSLNQIYTQIKNDLKGE
                     LLLLNNLTTSIALDIGLKMTMNAPFKQIAEDAADKYSINVQYFEGFSQSSNIIISCMS
                     GLGISDKLKDVFRHHLSDESIEVFTKDYRELRELIDQNDETYFDKTKLVITTSDLPRS
                     FSIPNINVYDILDGDGAANLKHILSSEMKPVEFNRLIQELIKFFSLEGIADRLNFLNP
                     TIVMSELETVISKYENYYHFTLNGRVKLNLYMHTALMMERLFLARTEKAEEPNYDMKP
                     DEDEFYYVSRSIFQSMEMKYNFKVNAYELSLLYELLGPYIQK"
     gene            18335..18835
                     /locus_tag="ACG8KR_000878"
     CDS             18335..18835
                     /locus_tag="ACG8KR_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFQ0200273.1"
                     /translation="MIKPMFLKVGTGIAYMKVNRKKGINHMKKILIATHGHLASGFLS
                     SIQLLTGKVQEITVINAYVDECDYEQELNVFVNQVNEQDQIFVFTDLFGGSVNQKITK
                     TFLEKEIAVTILSGFNLPILLEIVLATKELTKEDIQELVNKCQQEIKMTILDEVHVVN
                     EEEDFF"
     gene            18847..19341
                     /locus_tag="ACG8KR_000879"
     CDS             18847..19341
                     /locus_tag="ACG8KR_000879"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFQ0200274.1"
                     /translation="MIKAVRIDERLIHGQVAMTWTKSLNLTGLVVASDEAATNDIQKM
                     TLKMAAPSNVKVIIKTVDDAIHLLKDPRAKDMRLMVLVPNVKDAVKVAKAFPTEIELM
                     NVGNAGKMTPPSEDKKTLSKEVMLTTSEIQDLKELVELYPDTVFQPTPAMEKRSAISI
                     LSDF"
     gene            19363..20172
                     /locus_tag="ACG8KR_000880"
     CDS             19363..20172
                     /locus_tag="ACG8KR_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFQ0200275.1"
                     /translation="MYDAFVVALAVFIGVAGHEMFGMSMLSRPIVVAPLAGALMGDLQ
                     TGLIVGASLEAIFMGVVNIGISSTAEPSLAAGLATAFAIKMGGNVDAIIPIVFPLAVL
                     GLQLMNMIFSFVCGPMAAKFFKFAKSGDQKGIINLHFVMWFVHYALYALIPFFAVLFG
                     ADVVKSVLDNIPEVIMNGLTVAGNLLPAVGMAMLLRMLWSKNIAVWFFFGAVVMAYLE
                     LPLIAIAAVGTIIAIVIAQRDLQLKNISVHAVQSDAAVNATLDQEEEDFFQ"
     gene            20169..20990
                     /locus_tag="ACG8KR_000881"
     CDS             20169..20990
                     /locus_tag="ACG8KR_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFQ0200276.1"
                     /translation="MKLADGISKDEKKLVRKMFWRSMTMYASVNPVTMGGGGFAYSMM
                     PFINHFYKSEEDRAAALERHTQYFSTTIPCASFVMGIAASMEKENSEKAGFDVESINA
                     IKTSLMGPLAGIGDSLFWGVWRVVTAGLAINLANAGNILAPIIFLVMFNIPNYLMRYY
                     GGVLGYSLGSKYIEKLYANGLIEILTKAASILGLIMVGAMTISMVKFNTVLSFSMKGE
                     ELMNVQEILDSIFKGIVPLLVTLGCFKLLDKKVGVIKIIFGMVVLGILLSLLGIA"
     gene            21041..23140
                     /locus_tag="ACG8KR_000882"
     CDS             21041..23140
                     /locus_tag="ACG8KR_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HFQ0200277.1"
                     /translation="MTINEVIYSPKHPLFNAELNDGNVTTSNEYSYYRNIPDVKGLTF
                     DVSNQKTLGNLDVCGTIQTLTFYRENLLTEEKPGVWVNKQLSQTNSLSLKVEVNGIVY
                     HLSESDHRIEVDVIQDCLPRYIHHYSTFKVIVVSFAPILANKRLSMLVQQVYIVNETH
                     ESLQVSVKDVPLYQEKYSDQQNVLISQKGNKDTIAQQEVASFAIAFVDPNAFEEVMTF
                     QESDIEKWLKETLNYFAQLFGQLSMPNDRVVHLYRRALYQSFSSFGMNRQGQVVGSNW
                     GSYPATNRIWNKDMYYASLPFTMFEPELCQKTVLWFDQYGVKFPGSKFTGGINHSLSN
                     SLSSALLASLYFEHTGDTKFFERYPQVLLNASRIIEDILAQRRSGEPMLFPSVWLSDA
                     FALGKYHTGSNLCLWKACIGLAEIFEALEQLSLSKRYKNIACKLKESICKQMTIDGPF
                     GEQFLEGIGDEEKTTYSVKHYQKPILEQGLIFLSDVIVDGKINLLMHDGEESDTTLMP
                     FYQFLDNKDHLYQNTMAFSASSFNPTYSEEIKGITWGLESGATFPGFITVLMSELHNP
                     QAFVTRLEELICLADLDGSWWWWPYRLEAQQGEVVRDFGCGKCGWASGLFVSLMISQY
                     FGLKFKKGVLQIDPVDNLTFSWKNLKFGQAFISIECTQSELSISNLSEKPLKISDTKK
                     ILHVEKGKTIHIQRRKR"
     gene            23137..24255
                     /locus_tag="ACG8KR_000883"
     CDS             23137..24255
                     /locus_tag="ACG8KR_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 172 protein"
                     /protein_id="HFQ0200278.1"
                     /translation="MSILKNITQFKKIQSRAITPENPTGEKGKAAMLPSELGPSRKGR
                     GAIPLTSGEETVIADITGTGIIRHMWMTIRDRTEFGSFVLRDLILRIYWDGSDSPAVE
                     SPLGDFFCNGFGERCEVNSLPIVVNPTGGFNSYFEMPFQTSAKITIENLHPKDMSSFF
                     YTINYALVDELEENSLYFHANWHRERITKLQEDYKLIDRLKGQGYYVGTYLALTCLER
                     YWWGEGEFKFYIDGDSEYPTVTSTGSEDYFGGAWAFHEYDEFGRPHAKNYSTAFLGYP
                     YQSNRDATRDRFETGKLNAVHAFGDDAMPRHGLYRWHLQDPICFTEDLSVTLQQIGND
                     DVRLFERQDDVASVAYWYSTEASGNTKRHFTDRERLPR"
     gene            24294..25172
                     /locus_tag="ACG8KR_000884"
     CDS             24294..25172
                     /locus_tag="ACG8KR_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFQ0200279.1"
                     /translation="MIYGAIEAGGTKFICAVSTEELTILERVSFPTTTPVETMKQVID
                     FFKPFKEQLAGIGVGCFGPIDIQRKSKKYGHITSTPKLAWQNFNFVGTLKQHFNIPIE
                     WTTDVNAACYGEYVAGSGKGLAGVAYFTVGTGIGGGALFNGTFVEGFSHPEMGHMLIK
                     RHPKDNFSGNCPFHQDCLEGMAAGPAIEKRLGVKGQNLLADDSFWQIEAFYLAQCAYN
                     TTLMFSPDRIIFGGGVMKQEHMKKKVQDKFVELINGYVEIPPIDSYIITPELGDNAGI
                     IGGLALARKAVRNKQP"
BASE COUNT         8046 a         4428 c         5389 g         7553 t
ORIGIN      
        1 cgattttctt gaaacaataa aaaatcaaaa aaaatactct cctaatagtc catcttcttt
       61 tgattttatt tatataatga aatcatctta tgaaaattac tacttcatgt ttagacaaga
      121 gtagggagga ctaagaataa atgtgttcaa aaagacgttc aaaacaacta aataaacacg
      181 cttgtcgttt agataaggaa cggtcgtgtt ctgaaaaaca ctacgttaag tttatgatgc
      241 gcacgatgct gatcagttta tctgtaggtg tctatatgca tgtacccgtt caggcaacga
      301 cacaaaccat caaggtcgta cgtacctctg tttctgagac cactgattcc cctatcacta
      361 ttactcatac tttcactgat gcaaatggaa aacagctact tcctgcattg acttcctata
      421 ataaagtcag ttatcctcca acgataaacc actactggtt aattcctaac aaaacattat
      481 ggacatcgga aaccggaagc agtgtgccgc tttctatctt ttccgatatg actgatccta
      541 caaaaggtga tcagttaaaa cagatcactg atattttgaa tcatgctttg acagatataa
      601 aggacggtgg aagcgcacaa gtttcttatg tatatgaaaa agattattca cagtttgaaa
      661 gaaagaaaat cgatgttctg gcagggacaa aagtttcgat atctgatctg gtagaaaggc
      721 tgaagaacgc agatggaaca caaggagata tgtccaaagt aatttcaaac aatgggaata
      781 tcgtggatac gactaataca ggaacatcta ctatttcctt gtcctatttt gatccaattg
      841 ctttaaaaac aatgactgca tctgctgtga tccatgttcg tgctgatcaa acagctatca
      901 aaggaaaaga tcttcactta agaacaggag agagttacgc ccctaaagat cttgttgaat
      961 atgtaacaaa aagcgatgga acttcaggaa ataaagaaga aatcaaaatg aacgggacag
     1021 tagatactac aactccggga gaatatcccg ttgtattaac ttatatagac tcgaaaacat
     1081 caaatgtggc agaaacaaca gctgtagttt atgtagaagc atctgctgct tcatcgactg
     1141 tcatcactca ttttatagat agtcaaacaa ataaagaact tcaagcagaa acacatatga
     1201 gtagtgtaaa tcgtattcct gatttgcgcc tacctgctga attttctgat cgagtgccaa
     1261 atatagattt atcgacttat gaacaaaatg ggaaagtcac ttctatttct gattggatgc
     1321 aagcaaacgg attagacaga aaaagtcact ggtcggagat ccttgaagta cttcactcag
     1381 aactgatgaa taataggaag caatcaggag gggaaattac ctttacttat gtttatatac
     1441 cagacaatcg ttcattagaa ggtcatgatc ttattgttca tccaaacgaa aaaataaaca
     1501 aagaagagtt gatccaatca gcaacaaatt ccttaggtaa acccgtaccg ttaaaggatg
     1561 tatcggttac aatcaatgaa gtaccattgg aggatggtta tttgaccaaa caaacaggtg
     1621 attttacgat tatgtatagt tatcatgacc cgtattcact ccaagaactc catgctcagt
     1681 cactattaca tgttttacca ttaaacaaac agatagatca aaaagaacaa aaaagacaag
     1741 aagaaagctt gtcagaaaaa aaacaacaaa tggctcatgc agtttctcca acagggaaag
     1801 taaaacaaac aagaagccta gagttagcaa gaatatctag aaaaatctca gaatctaata
     1861 aaacgataag gcaagaagct catttggccc cacaagtttc tggattggaa aatgaaattt
     1921 tagagcctaa aataaaacaa caatcagaaa aagaatttgt tgctaaacag tcggaagatc
     1981 agaaggaatc agatgaccag aaggaaacga agaaaaaacg cttgtcaact attgattcca
     2041 ccgatgagca tctgcagccg caagtaccac ttcctagtcc gggaacaccc gcacctggtg
     2101 gaggtattcc ttcttctgct ttaggagatt tcatgcgagg tatggcagga acatgggtat
     2161 acgctaatcg aaatgaattt gacgcagatg actggttctg atttatatct ctatctgtta
     2221 tagtcaaata agtagctgag tcatatgtta tttggcataa tggagacgag gagggataga
     2281 aaaatgttag ataaaacgtt accttacgca gaaatctgga tgacaagacc acttcaacaa
     2341 aatgttccaa tcacgccttt agcggaaggt tttgaatttc gattttatca aaaagaagat
     2401 gaatctgctt gggcggagat tgagacttcc gttggggaat ttcaaacgac agatgaagca
     2461 cttgagtatt ttcaacaaaa ctttgctcct tatcctgaag aacttgaaag aaggatgttt
     2521 tttgtagaga caaaaaaagg agaaaaagta gcaacctgta ccgcttggcg aaaaaaatca
     2581 agagagcaag tgtatccggt ttttcattgg ttagcagtga aaccagatta ccaaggaaaa
     2641 gggctcgcaa aagcattgac tgccagagtc ttgcaagaat ttcctaccct ccatacggac
     2701 ggaccaatct atcttcacac acagacatgg agccatcagg cgattgctct ttataaaaaa
     2761 atgggtttta cttttattgc tgaaaatcta gacggcacac ctaatccaga ttatgaaaaa
     2821 gttatgcagg ttttaagtaa attagataaa taacgaaatg gtgaaataag tcctcagtat
     2881 tgggataaaa aacgaaatag tcattcctca tatcttactt ttttgagtta atactatcgg
     2941 ataactaatg gcatgccata cattcaaaaa agaaggggca gtgacaaaag tattgtcact
     3001 gccccttctc ttgtttttta gcatgtttta tcgtttgatt tttttaggaa taaaattctt
     3061 gatgaacgta aatttttggt aatcgtttcg actatagagg taaccaaata cactgaaaac
     3121 aactgctcca ataaaattaa caaataaatc cttcatagta tccatgattc ccacgtctaa
     3181 gtaaccattt tggatgattg ttttatctgt tttaccatct gcattttgac tttcaatgat
     3241 tgtccgagtg atatctggaa catgatagac agtgtttccg tctttactta actccacact
     3301 attgatttgc tggatgatcg tatctttttg catatcgaat tgcagtaatt tatctgctga
     3361 aaattccaca aactcccaca taacaccaac tgtcatcgaa aaacagaagg aaacaactgc
     3421 tagtaataaa ggagtcaagt tcatggtttt tgtattttta ttgagcaaat aaacaaggga
     3481 aaagccgaca cccccagcta aaaatccgct gaatgcatgt agaaaagtat cccacattga
     3541 aaaatagcca taaaaatcac ttaactctcc taaaactgtt gaactaaaaa taaaagataa
     3601 caaaatgatt tctagcatat ttggaatctg tatctttaat atcttttcca gaaaataagg
     3661 gatttcaaat aaaattaatg tcagtaataa aagcattgta tttccccagt gttggttcca
     3721 gacctgatcc actagcataa taacgacgat gatacgtaat aaaatataga ttccgtttgt
     3781 gattctacct gcagtttttg tactgttcgt aaaaaaggtt ttcaaatgat tcataaaata
     3841 actccttcat gtatatcctt aattatacta agaagcgtgt gtttaccaaa gctatatgac
     3901 ttaaagaaaa acagagttga tattgtgaaa aaaatcactt aatatagtat aaggagaaaa
     3961 gggggataca aatgaaaaaa ttagttgctg gtttattaac agccgttgca cttcttgcta
     4021 tgagcgcatg cggcgcagat cagaaaaata cagcgcagac aagttctagt acgaaggaat
     4081 ctgccgaagt atcatcaggc gcttctgaac aagtatatac agatccaaaa gaaatgaaag
     4141 aatcttatga tgtcgtgatt attggagctg gtggtgcagg gatggcagca gctatcgaag
     4201 caaaagatgc tgggttgaat ccagtaattt tggaaaagat gcctgttgcc ggaggaaaca
     4261 catctaaatc atcaagcggt atgaatgctt cagaaacaaa atttcaaaaa gaagaaggaa
     4321 tcaaagactc gaatgatgcc ttctatgaag aaacgctaaa aggtggtgga ggaacgaatg
     4381 ataaagaaat gcttcgatac tttgtcgatc actctgctga tgcggtcgat tggctagatc
     4441 aaaacggtat caaactggac aacttaacga tcactggagg gatgagtgaa aagaggacac
     4501 atcgtccgtc tgatggctcg gctattggtg gatacttagt ggaggggtta ttgaataacg
     4561 tccatgagcg ggaaatccct atttttgtca acgcagatgt gacagacatt aagaaaaatg
     4621 gggaaacggt caatgaagtg actgtacatg ttcaaggaga agaaccaaaa gaggtcaccg
     4681 gaaaagcaat cgtagttacc acaggcggtt ttggtgccag caaagaattt attgaaaaat
     4741 atcgtcccga tttagctgat tatattacga caaatcaaga aggatcaact ggagatggta
     4801 tcaccatgat tgaaaaatta ggtggacaaa ccgttgatat ggataaaata caaattcatc
     4861 caactgtgca gcaggatgag ggggtcttga ttggtgaggt tgttcgcgga gaaggagcaa
     4921 tcttggtcga tcaagagggg aaaagatttg tcaatgaaat ggatacaagg gataaagtat
     4981 ctgcagcaat tacagcattg cctgaaaaat cagcttattt gatttttgac caaggcgtgc
     5041 gtgatcgtgc cacagctatt gaattttatg accaaaaagg atatgtcaca actggaaaga
     5101 caatggaaga attagcagaa aaaatcgatt taccaaaaga aacactagca aaaacagttg
     5161 atggatggaa tacggatgtt gcggagaaaa aagatgctca attcaaccgt gccacagcca
     5221 tggaacatga cttatcgtct ccgaattatt atgcgatcaa aatcgcacca ggaatccatt
     5281 acacgatggg cggtgtgaaa atcaatacga acactgaagt attagataaa gaaaaccagc
     5341 caattagggg gttatatgct gctggtgaag tcacaggtgg cttacatgga aacaaccgga
     5401 tcggtggaaa ttcagttgcg gagatcatta tttttgggcg acaagcgggc atacaagcag
     5461 ctcgatttgc ttcggaaaat gaatgaaata gaacgtaaat aaaaagaggt tgtgaaatca
     5521 gcgttttgag ctaataccga gggtcagctg attgaacact gtttagtgaa gaggttgtga
     5581 aaaagctgcc ctgcatcaag taataagaac agccaagtaa aaacagcttc ttatgttttt
     5641 aacttggttg gacttattac cgcagatgca agcttttgaa caccgtttat tcggaaaaag
     5701 agcgtgggac aaaagtaaag aacacttttg tctcacgctc taaacacgta taaacggcgg
     5761 aagcagaaga aattccttcg gaaataagct gaaattcaca aaaatttgaa aagcaatttt
     5821 cgtgaatttc ttcttatttc ttggagttaa acgcttctgt cccagcctct tttttgatca
     5881 gcatgatttt attcagttat cttcaaaatg atttttccga tgtttttgtt tgcttccata
     5941 tattcatgcg cttgtttgac tgcgctgaat tggaatgtac ggtcaatgat tggccgaatc
     6001 ttgccatttt ctagaaaagg cccagctttt tgcataaaag tttgtgcgag ctctgcttta
     6061 tactgatcac ttctaggtgt caataatgta ccaagcagtt ggacacgttt cccaatcaat
     6121 acaccaaggt ctatctcagg cacgatggtc ccacctagca taccaatcaa gacccaacgt
     6181 ccatcaacgg ctatagaagc taaattcttt tcccagtaag aagcgccgat gaaatccaaa
     6241 ataacatcga ctccccgacc ttcagtgatt tttcgtacct cttcttgaaa gtcttgtttt
     6301 ttatagttga tcaattcatc tgcaccaagt tctttacaaa aggcgagttt ttcttctgat
     6361 cctgcagtaa cgatgatttt cgcgtgagaa agctgtctag ctaattggat cgctgctgtt
     6421 cccacaccac ttgctcctgc atggatcaag acagtttcgc cttcttgtaa ttttccaagc
     6481 cagtaaagtg tttgataagc agtcaaaaaa acttctgaga ttccagcagc agaaacatag
     6541 tctagcgtat cagggagaag gattgcacga tcggcaggca tcacagcata ttcggcataa
     6601 ccacctagat tgactaatcc gtatactttt gtcccaggag taaaagtgga gatatcactt
     6661 ttattctcaa ctacctctcc agctacttcg actcccaaaa tagggtaggg aggtttcaaa
     6721 gagactgatt ctcttttcat aatatctgtt cgattgacag ctgctgtatg gactttgatc
     6781 agtaattcgc cttgttttgg tttaggaaca ggcacttcaa tttgttctaa tacatctaca
     6841 gaaccaggat gcttgattga aatggcacgc atggatctat tcctcctttg cccattcttg
     6901 gaatgctgta ttcaactctt tgctaccttc agaaagtact agagtcgtac gagaaatatc
     6961 cgtttcttcc actagagcgg caagtacagc agcaactgta gagcgagtga tcgtttttga
     7021 tgccttctca ttaggcttag ttaattgaat ggaagtaact tcttcatcgt tagtcaatgt
     7081 gactggttga agaatgacaa aatctaagtt agttcgtttc agccattcat ctgcataatg
     7141 tttagtaata taataatctg tcatggactc aggccatttt gctggttcat ctgcataaac
     7201 agcactgacc atcagatagc gagagacatg cgcattttca gctgcgatca ctgttttgat
     7261 tgcgccatcc aagtctactt gcagaagatt ctttccttga gagccggcag caaaaatcaa
     7321 tacatcgatg cctttgaatg cttcggtcat tttttccacg gaccaagtta aatcaaaaga
     7381 gacatacgaa acattctctt tttttactaa tgtttgggaa gcaacatctc tcacccctgc
     7441 aaaaatttca tgggaacttg gagctaaatc ttttatcagg tgtcggccaa tttgtccgtt
     7501 tgcacctaca acaaaaattt tcatttggat catcctttcg acgtcaaatc tttccattca
     7561 atagtaacac caaaagagaa aaaaatcaga gatcggctac gatgactatc agttagtaag
     7621 agaagggtgc ttgaagtatc ttaacaagac attgcatact gctgcagtcg caagacctgc
     7681 gacgattcct tcaggtatac cacttgtaaa aataaccgct agaatagctg aataaacagc
     7741 atttaaattt gttccgatga ctttggcata atcttgctgg aataaaaagt agatcatatt
     7801 catcactaaa atcgtattgg tcaatgaacc ggaaagtcca gctaagaata aagaaacgga
     7861 atctgctttt tgtttcatta gtttgcgcag acctttataa acgaaataag gaaccacccc
     7921 aatcaagatg cgaggaataa acgcgatcag caaggctttc cagcttccat gttctgttcc
     7981 gatgactggg ataaacggag aaaagacaaa agacaaaggt gtttggataa ctgtgctacg
     8041 gatcatgctg atcagtccaa atgtaccgcc aagaaaagca cctagtcttg gtcctaaaac
     8101 gatcgaagca atgattacag gaatatgcat ggttgttgca ttgattggac cgatagggat
     8161 aaaacctaaa ggcgtaacag ctaataaaat caaaatagct aaaaacatcg cagttaatgt
     8221 aaagtttttt gtatttttca taagtactcc ttttttctaa gcatttcttt cattaagagt
     8281 cagcatgacc tgttcgatga tcgtctgaat atcagctaag gcaccttttc caaaatctcc
     8341 acaagctaaa agagattctc ttggttcaat ttcctgatag ccgacagatc tcaatgtttc
     8401 cagattacgc tgagtgatcg ggttttgata catattcgtg ttcatggcag gggcaatcaa
     8461 ttttggtgtt cccactggaa gcgccattgc gactgttgaa agaagatcgt ctgcaatgcc
     8521 gtttgctagc ttgccgataa tgtttgctgt agcaggcgcg atgagaaaca agtcagcttg
     8581 ttttgctagt tcgatatgat tgattacctg cggatttgat tcttgcatga cgtctgtatg
     8641 gactgcgcgc ttcgacagag attgcaaggt taatggcgtg ataaaatgtg tgctgctttt
     8701 agacataatc acatctacct gggcacctaa tttgacgaat tgacttgtaa gatcagcgct
     8761 tttgtaagca gaaatacttc cagtaacgcc tagtaagatc tttttcattt tgattcctcc
     8821 agtctaattt tctcagtaat caatgcagca atctcagatt tactttgagc ttcttccact
     8881 gttccatctt tagataagag atagccgtgg tgtgtctcgc catggatttc cgtcaaatca
     8941 tttgcgaaaa taaaatcagt tttgttattt atcagactat tttttgcgac tgccagaaga
     9001 gattcctttg gtacatctac taatagtttg aaaccaatga tttttgcttt tggctgccat
     9061 tcacgtagat aagaaatgat ttttggtgtt ttcttcaaaa cgagcacaag atgctcggtg
     9121 ttagaagaga tctttgaaac agtggtatct gtctgttcat tttccgcaaa ccaattgtcc
     9181 agttcttctt gcgttgaaga agtgggtagg ttttttgcca gctgttcttc tgaaaaacta
     9241 aaagcaggag taaaatcact gactgccata ctgtggataa tcgcatcgta gtgttctttt
     9301 ttcgttagtt cttctaattg taaaaataag tccagtgcag attcgatcgt ataaatcgtt
     9361 atattggagt gtcttttcgg tttcagtgct tgtttcgtag tcacgtaatc gattgtggta
     9421 tccggattgg cagctaaata atcagctaat gcttgcccta atcgtcctgt cgaatgattt
     9481 gtaatagcac gtacttgatc gattttttca gtcgttcctc cagctgtgat tagaatcttc
     9541 atttatggtc acctacattt actagtgttc ttccttggtg tgttccagca agtagtttag
     9601 atgctgtttc aggcaattga tccagagtaa tctcttggac gaccagttca tctgcaatct
     9661 gcaaatccgc catctgttgc cataaagaaa gacgttgatc tattggtaca ttgacagaat
     9721 caattccaat caactggatg tttcgcagaa taaaaggcaa gacagtcgcg tttagtttga
     9781 ttcccccagc attgccacat aaagctactg caccgttata agagatcaat ggaagaagac
     9841 tgcttagctg ctcgccacct actgtgtcga tgacatagtc gatttgctgt tttcctaaag
     9901 cttttgttgt ctctggcaga aattcatcag gtcgtacgac ttgcaaagca cctagacttt
     9961 ttagccatgt gacagactct tcttttctgg aaaaggcaat gattgactgg aatcctagtt
    10021 tatgtaacag ggctatcgct gtactgccta caccgccaga agcaccagta acgacaactt
    10081 ttttatcgga ggtcatacct tgacggatta aagcgttcac acacaacata gcagtaaacc
    10141 ctgctgtacc aaggatcatc gcttgacgca gcgtcatatt ttttggtaga ggaactaacc
    10201 agtctcctgg tactttttga tattggctat aaccgcctgg atgggtgacg ccaagcccaa
    10261 aaccagtagc aagtacaaga tcttcttttg aaaaacgtgg attgttactt tggacgacag
    10321 ttccggataa atcaatccct ggagtcatcg gatatgttcg tatcacctga ccagactcgc
    10381 tcattgccaa tgcatcttta taattgacat cagaataggc aatttttacc aagacttcat
    10441 tttcagccaa ctcagtaaat ggcagatcac tgattgctgt atggaaaaca ggttccttta
    10501 ttacctgaaa agatcgaaaa gcgttcattc gtgagacact ccttttaaaa ttttctttta
    10561 tcataccata aagcctactc ttttattctt gttcttccgt aaatttagac aaaaattagg
    10621 tgaaaaataa agaatcaaac aactagaatt tattccaaaa gataaagaaa tattcagcaa
    10681 aaaaaatttt acaaagggtt gcctttttca ataaatttcg tatactaata cgagtggtag
    10741 taaacaggaa gtttactatt agaaaacttc gattcggagg aaattatgga aattggtgag
    10801 aagttaagaa atttgcgcat ccaaaagaat ttgacacaag aagaactagg cgaacgcaca
    10861 gatttgtcaa aagggtatat ttcccagttg gaacgagatt taagttcacc atcaatggaa
    10921 acattctttt ctattcttga agtcttaggt gtgactccag aagaattttt tcatcaagaa
    10981 gcagcgaatc tgcaagtggt ttattcaaaa gaagatcata cggtttattt cgatgaagaa
    11041 aacgggtatg aattggaatg gctcgtggct gattcgaatg aaaaagaaat ggagcctgtc
    11101 ttgctgactt ttgaaggtgc aggagaatat aagacgtttg aaccttcatt atctgaaacg
    11161 tttatttttg tattagaagg ggaattagag ttaagcttag gagagacggt ctatgtggca
    11221 aaagcaggac agtcgatcta ttatcaagcg acaaaacaac atcaattaag gaatcatctc
    11281 aaaaaacgaa caaaagtatt gattgttgca acgcaatcat atttgtaata tagggggtaa
    11341 aaacgtgggg aaaccaatca tttcatttga tcaagtagtg aaacgctatg atgatgaaac
    11401 agtattaaaa aaagtaagtt ttgagatcga acaaggaaaa ttttatacac tgcttggtcc
    11461 ttccggctgt ggtaaaacaa cgatcctacg tattatagct ggattcaccg aagctacaga
    11521 aggggacatc tattttgaag ggaaacggtt gaatgatttg ccagctaata agcgacaagt
    11581 aaatacagtc ttccaagatt atgcactttt tcctcatatg aatgtttttg ataacgttgc
    11641 ttttggattg aagatcaaga aaatgtccaa atcagatatc gaaaagaagg taaaagatgc
    11701 tttgcgtatg gtccagcttc ctggatacga aaaacgggag attagtgaaa tgtctggtgg
    11761 tcaaagacag cgtgtagcga ttgcacgagc aattgtaaat gaaccgaaag tattgctatt
    11821 agatgaaccg ttatcggcgc ttgatttgaa actacgtaca gatatgcagt atgaactgcg
    11881 ggatctgcaa caacgattag gaatcacgtt tatttttgtt actcatgacc aagaagaagc
    11941 gttggcaatg agtgatgaga tttttgtcat gaataaaggg aaaatcgtcc aaagtggtac
    12001 acccgtagat atttatgatg agccaatcaa tcatttcgtt gctgattttg ttggggagag
    12061 caatatcgtg gacggtgtaa tgatcgaaga taatctagtt gagtttgttg gtaagcaatt
    12121 tgaatgtgtc gatggcggga tgcgtctaaa tgaaccagtt gaagttgttt tacgcccaga
    12181 ggatttgacg atcacgacac cagataaagg aaaactagtc gtaacggtcg atactcagct
    12241 gtttcgtggt gtccattatg aaatcatctg ttatgatgaa cagcaaaatg aatggatggt
    12301 ccattcaacg aaaaaagcta aagaaggaag caaagtagga ttggcctttg agccagaaga
    12361 tatccatgtc atgcgtttca acgagtctga agaagaattc gatgcacgtc ttgacagcta
    12421 cgaagaatag gggggacaag aatgaaaaca atgcgcagga tctattccgt cccttatatt
    12481 ttgtggctcg gtttattcgt gatcgcgcct gtattaatga tcatttatca atcttttttt
    12541 gatatgaatg ggcaattcac gctgaataat tatctatctt attttacgtc ggggacatcg
    12601 ctttctatga cgatcaattc tgtatggtat gcatttttga tcacgttttt cacgctgatc
    12661 atcagttacc cgactgctta ttttttaaca aaactgaagc acaagcaatt atggctgatg
    12721 ctgattattt tgcctacatg ggtgaatctg ctgcttaaag cttacgcatt tattgggatt
    12781 ttcagtgtgc acggcagcgt gaatcatttc tttgatttta tcgggatcgg gccgaaacag
    12841 atattattca cagacttcag ttttctgttc gtggcaacgt atatcgagat tccatttatg
    12901 attatgccaa ttttcaatgc attggaagag atgaatcctt ctttgatcag tgctagtaga
    12961 gatttaggcg cgaatagctt tgagaccttc cgacgagtga tttttccgtt atcattaaat
    13021 ggtgtgaaaa gtggtgtcca agcagtattt attccttctc tttctttatt catgctgact
    13081 cgtctgattg gcggaaatcg ggtgatcact ttaggaacgg ctatcgaaca gcatttcttg
    13141 gttacacaaa actgggggat gggttctaca atcggtgtga ttttaatcat tgctatgttc
    13201 atcgtgatgc tcctgacagg cgagaagaaa aaaggaggtc gctcataaat gaaaaaattt
    13261 aaatggtcgt atctttattt agggatcgtc tttttattct tatatgcacc gatcttctat
    13321 ctgatttttt attccttcaa tgaaggagga acgatgaaca gctttacagg atttacttgg
    13381 gaaaattatg aagctgtttt tgaagataca cgtttgatca ttatcgtatt gaatacgttc
    13441 atgctggctt tcttatctgc attgatcgcc acgatcatcg ggacatttgg ggctatggga
    13501 atctattaca cgaaaaaacg acgtgcacgg acagcgttgt tgagcatgaa taacatctta
    13561 ttagtctctc ctgatgttat tatcggtgct agtttcttga ttttctttac actactagga
    13621 ttcggattag gttttcagag tgtattgctt tcccatattg ctttcagcat tccaatcgtc
    13681 gtcttgatgg tcttgccaaa attgcaggaa atgaacgatt caatggtcga tgcggctaga
    13741 gaccttggtg ctaataattt ccaagtagtc aaaaatatca ttttgcctta tctttctcca
    13801 ggaatcatag ctggatattt tatggcattt acctattctc ttgatgattt tgcggttaca
    13861 tttttcgtca caggaaatgg ctttacgacg ctctcagtag agatctattc tcgtgcaaga
    13921 caaggaatca gtttagaaat caatgcgcta agtgcgttag ttttcttgtt ctccatgata
    13981 ttggtcattg gatactactt tattagtaaa gaaaaccgtc caaaacggct gaagaaaaac
    14041 agaagagtac gcgagggggt ggcacggctg cgatgaaaaa attacaatct ctgtttttag
    14101 gtattctcat cattattgcg gtgctgctgc ttggtgttcg tcaacttgaa aaaacaagtg
    14161 gcatgagtgg agcaaaggta cttaccatct ataactgggg agattatatc gatcctagtt
    14221 tgatcacgaa atttgaaaaa gaatatggat acaaagtgaa ttatgaaaca tttgattcta
    14281 atgaagcgat gttcacaaaa atccagcaag gtgggacaaa ctacgacatc gctataccaa
    14341 gtgaatatat gatccaaaaa atgatcaaag aaaaactagt acttccatta gatcattcga
    14401 agatcaaagg attaaataat attgatccac gatttttaga cttggatttt gatccagata
    14461 atcagtattc gattccatat ttttggggaa ctttagggat cgtatataac gataaattct
    14521 ttgatgcaaa tcaaattaag cattgggatg atttgtggaa gccggaatta aaagacagtt
    14581 tgatgctgat tgacggtgca cgagaagtta tggggctttc tttgaatagt ttaggctatt
    14641 ctttgaatag taagaatatg gcacagctga cagaagcagc aaataaattg aatcatttaa
    14701 caccaaatgt caaagcaatc gtggcagatg agatcaaaat gtatatgatc aatgaagaag
    14761 cctccgtggc agtgacattt tctggcgaag ctgccgatat gatgtgggaa aatgaacatt
    14821 tacattatgt gatcccacca gaaggatcca atctttggtt tgataatatc gttatgccaa
    14881 aaaccgtgaa aaataaagaa ggtgcctatg atttcatcaa ctttatgcta gaaccggaaa
    14941 atgcggcaca aaatgcagag tatattggtt attctacacc aaatgaagaa gcaaaagcgt
    15001 tattgccaaa agacatttcg tcggacgaac aattctatcc atctgatgac acgatctccc
    15061 atcttgaagt ctacaaagat ctaggatcga aatacctagg catctataat gacctattct
    15121 tagaattcaa gatgtatagg agataatggc aaaactcagt aagaatgggg acttatcaac
    15181 gttctaaaga gctaaaaatc gtcctttttc tatctgagtt ttccaatctt ctaccaaaag
    15241 cggatgaaac gccgcttttt ttgtgttatt ttattttttc taccacgttt ttctaccaga
    15301 gaaaagtgga caaaaccatt gtaaaatagg gcccgagacg ggctagtaaa tctaagattg
    15361 aaaaagttac agaaggataa agtaattgga aactatgcaa atatacttaa cccttctgta
    15421 atctattaat aaaactaata tatgaatcca gttcaatatt tgtagctatg aaaaaacata
    15481 aaaatcatat tagataaaaa ctaaattggt agataaatta ttttgtcaga tatctgaggc
    15541 aaatattcga ctatttgtgt ttcaaagtaa aaattggaat acaaatatag aatagacata
    15601 ttgcagccga tagtttatta tgcttttaga cacattaaac tggaggaaaa aatgaaaaag
    15661 agcgctaaag atcgagtttt tgatgagctg atacgactaa cagggaatat gtcttctgtc
    15721 actgccgaga aaatttcgaa gaatctaggt atctctcgtc agaatgccag ccattatttg
    15781 acgagattag tggaagataa aaaggtcgag aaattaccgg gaaagcctgt tttgtggaag
    15841 cctttagatg aatatgcggt tgtagataat acggagcaaa taaacgaagc ctttcattct
    15901 gtagttggtc atgacggaag tctgagagag gtcattcaaa aatgtatagc cgctgtcaaa
    15961 tatccgccta acggactcag tgtattaata aatggagcca cgggtgttgg aaaaagtttt
    16021 ttagccacaa aaatttttga atacgctatc catgagcaaa ttattgaaaa agatgcacca
    16081 tttgctatct taaactgtgc cgattatgcg gacaatcccg aactgctttc ggcaacactg
    16141 tttggataca agaagggggc ctttacgggg gctgaaaagg ataccgatgg tttattggca
    16201 acggccaaca acggctatct ttttctcgat gaggtccatc gcctatcgaa agaaaatcaa
    16261 gaaaagctct ttctatttat tgatactgga aactatcgtc cagtcggtga aaatgtaaat
    16321 tggcattctg ctaaggttcg ttttattttc gccacgacgg aaaaaggtga aaattattta
    16381 ttagacacct ttgatcggcg tattcaaatt agtgttatgt tgccaacttt tgaagatcgc
    16441 cccattcgag aacgattaga gctgattcaa ttattttttc aaaatgaagc ggatgtttta
    16501 caaaaagata ttattgtttc aagagaagcc ttgtctttaa tgttatctca tccctttagt
    16561 gggaatattg gtaagctgaa gaatatcatt aaaatcagtt gtgcggatgt gtatagtcga
    16621 acacaagaag aaccattaac gattgggaaa aatgagatct tgattcagtt aaatatgctg
    16681 gaatccgaag tagaaagctt gccgattgct agtttattga taaagaaaaa tcaaaagata
    16741 aaaacggatc gaatcaaacc gtttgattat gattcgattt tggaaaaaat tactcagctg
    16801 ataaatgaag gtaatttcaa tgaaaatttt tcttcgataa agaatgaagt acagcatttg
    16861 gtgcatcaca ttgataagga ttctttttta tcaatgagta gaaaattgat gattcagatg
    16921 tttagaaagg tctgggtatc cgttttagga aaaacgtacg gattgacgac gagtggacct
    16981 gttgcagaaa ttattagcag atcctatgtc atttccccaa gctataagac agacttaacc
    17041 gggataaatc gtacattagc tgcaaatcta cctcggacga cttacttatg taatcagttc
    17101 ttggagcatt taccaccgct ctcagaaaag gacgagaact ttctaagact gcttattgtc
    17161 gtgactttat ctgattatgt agatgaatcc atcgagctga agggcctgtt gcttgctcac
    17221 ggagatagta cggctagcag cattcaagca gtagtgaatc aattatgcgg caattatgtt
    17281 tttgaagcgt tggatatgcc agtagagagt aatgtgtcag agattgtcga atcgacaagg
    17341 aatttcgtga aacggcaagt gaatacagat agtctagtgc tgatcgtaga tatgggctct
    17401 ttaaatcaaa tttacacaca gatcaagaac gatttaaaag gggagttact tcttctgaat
    17461 aatttgacga cttccattgc acttgatatt ggcttgaaga tgacgatgaa tgcgccattt
    17521 aaacagattg cggaggatgc agcagacaag tattcaatca atgttcaata ctttgaaggc
    17581 ttttctcaaa gtagcaacat tatcatttcc tgtatgtcgg gtttggggat ttcggacaaa
    17641 ttgaaagatg tttttcgtca tcatttatcg gatgaatcca tcgaagtttt cacgaaggac
    17701 tacagagaac tacgtgaatt aatcgaccaa aatgatgaaa cttattttga caagacaaaa
    17761 ttggtcatca cgacttcgga tcttccacgt tccttttcaa ttcctaatat taatgtttat
    17821 gacatcttag acggtgatgg agcagccaat ttgaagcata ttttgagttc tgagatgaag
    17881 ccggtagaat ttaaccgttt gattcaggag ctgataaaat tcttttcatt ggaaggaatc
    17941 gcagaccgtt tgaactttct gaacccaaca atcgtgatga gtgagctgga aacagtcatt
    18001 tctaaatatg aaaattacta tcatttcact ttaaatggcc gcgtaaaact aaatctttat
    18061 atgcatacgg cattgatgat ggaacgttta ttcttggcgc ggacagaaaa agcagaagaa
    18121 ccaaattatg atatgaaacc agatgaagat gaattttatt atgtttcgag aagtattttt
    18181 caatcaatgg agatgaaata taattttaaa gtcaatgctt atgagctttc gctgctctat
    18241 gaattattag gaccgtatat tcaaaaataa agtgtctaaa aaagtgtcta atctgatttt
    18301 ttcaggttag acactttttt ttatgtgaat taacgtgatt aaaccaatgt ttttaaaagt
    18361 tggcacagga attgcttata tgaaagtgaa cagaaagaaa ggaattaacc atatgaagaa
    18421 aattctaata gcgactcacg gtcacttagc ttcaggcttt cttagcagta ttcaattact
    18481 gacggggaag gttcaagaga ttacggtgat taatgcttat gtggatgagt gtgattatga
    18541 acaggagttg aatgtatttg taaaccaggt gaacgagcag gatcagattt ttgtttttac
    18601 cgatttattt ggcggaagtg tcaatcagaa gattaccaaa acctttttgg agaaagagat
    18661 tgctgtaact attttgtctg gctttaatct gccaattctg ctcgaaatag tattggcaac
    18721 caaggaatta acaaaggagg atattcagga actggtaaat aaatgccaac aggaaataaa
    18781 gatgaccatt cttgatgagg tacatgtagt aaatgaagag gaagattttt tttaggagga
    18841 agaaaaatga ttaaagcagt gcgaattgat gaacgattaa ttcatggaca agtagcgatg
    18901 acttggacaa agagtctaaa tttaaccggg ttagtagtag ccagcgatga ggcggctacc
    18961 aacgatattc aaaagatgac attgaagatg gctgcaccat cgaatgtcaa agtcattatt
    19021 aaaacagttg atgacgcgat tcatttgtta aaagatccgc gggcgaagga catgcgcttg
    19081 atggttttag tccctaatgt caaagatgcg gtaaaagttg cgaaagcttt cccaacggaa
    19141 atcgaattaa tgaacgtggg gaatgctggg aaaatgacac cgccatcgga agataagaaa
    19201 accttatcaa aggaagtgat gttaacgacg tcagaaattc aagacttaaa agaattagtt
    19261 gaactttatc cagataccgt ttttcagcct acaccggcga tggaaaaacg ttcagctatc
    19321 tctattttga gtgattttta gaaaaaagga aggagaaaaa ttatgtatga tgcatttgta
    19381 gtcgctttgg ccgtttttat tggagttgct ggacatgaaa tgttcgggat gtccatgctg
    19441 agtcgtccga ttgttgttgc tccgttagca ggagcgttga tgggggattt gcagacgggg
    19501 ttgattgtcg gggcgtcact agaagccatt tttatgggtg tcgtcaatat cggaatctct
    19561 agtacggcgg agccttcttt ggctgctggg ttggctacag cgttcgcaat caaaatggga
    19621 ggaaacgtgg atgcgattat tcccattgtc tttccactag cagtattagg attacagtta
    19681 atgaatatga ttttttcttt tgtatgtgga ccgatggctg ctaaattttt caaatttgca
    19741 aaatctggtg atcaaaaagg aatcatcaat ctgcacttcg ttatgtggtt tgtccactat
    19801 gcgctttatg ctcttattcc attctttgcc gttttatttg gagcagatgt tgtcaaaagc
    19861 gtcttggata atattccaga ggtcatcatg aacggattaa cagtcgctgg aaatctgctg
    19921 ccggcagttg ggatggctat gctgttacgg atgctttgga gtaaaaatat tgctgtgtgg
    19981 tttttctttg gggccgttgt tatggcgtat ctcgagttac cattgattgc aattgccgca
    20041 gttggaacga ttattgcaat tgttatagcc caaagagatc tacaactaaa aaatatatct
    20101 gttcatgcag ttcaaagcga tgctgctgta aatgcaacgc ttgaccaaga agaggaggat
    20161 ttctttcaat gaaattagct gatggaattt ctaaagatga gaaaaaattg gttcggaaga
    20221 tgttttggcg ctcgatgact atgtatgctt cggtgaatcc ggtgacgatg ggcggcggtg
    20281 gtttcgccta ttctatgatg cccttcatta atcatttcta caaaagtgaa gaagatcgag
    20341 ctgcggcgct ggaacgtcat acacagtatt tttcgacgac cattccttgc gcgtcctttg
    20401 tcatggggat tgcggcatcg atggagaagg aaaattctga aaaagctggt tttgacgtgg
    20461 aaagtatcaa cgcgatcaaa acgagtttga tgggaccatt ggctggaatt ggcgattcat
    20521 tgttctgggg agtttggcgt gtcgttaccg cgggactagc gatcaactta gccaatgccg
    20581 gaaatatttt ggcaccaatt atctttttag ttatgttcaa tatacctaat tatttgatgc
    20641 gatactatgg cggcgtttta ggctattcat taggctctaa atatattgaa aagctgtatg
    20701 cgaacgggtt aattgaaatt ctgacaaaag ctgctagtat cttgggtctg atcatggtag
    20761 gcgcaatgac gatcagtatg gtcaaattca ataccgtttt gagcttttca atgaagggcg
    20821 aagaactaat gaatgtccaa gaaatcctcg atagtatttt taaaggaatt gtaccgttat
    20881 tggtaacatt gggctgtttc aaattactag ataaaaaagt cggcgtcatc aaaattattt
    20941 ttggaatggt cgtcttggga attttattgt cattacttgg aatcgcatag taggaagctg
    21001 cagctactag gctgcagttt ttttagcaag gagggaaaag atgactatta atgaagtaat
    21061 ttattcaccc aaacacccat tatttaatgc tgagttaaac gacggaaatg tgactacttc
    21121 aaacgaatac agctactatc ggaatattcc tgatgtaaag ggattgacct tcgatgtaag
    21181 taatcaaaag acattaggaa accttgatgt ttgcgggacg attcaaacgt tgacctttta
    21241 tcgagaaaat ttactaactg aggaaaaacc aggtgtttgg gtcaataaac agctttctca
    21301 aaccaattcg ttatccttaa aggttgaagt aaacgggatt gtctaccatt tgtcagaatc
    21361 ggatcatcgt attgaagtcg atgtgattca agactgtttg cctagatata ttcatcatta
    21421 ttcgacgttt aaggtcattg tagtatcctt tgctccaata ctggctaata agcgtttaag
    21481 tatgcttgtt caacaggttt acattgtgaa tgaaacacac gaaagccttc aagtttcagt
    21541 gaaagatgtg cctttgtacc aagagaagta ttcggatcag caaaatgtgt tgatttctca
    21601 aaaaggaaat aaggacacga tcgcacaaca ggaagtggca agcttcgcca ttgcttttgt
    21661 ggacccaaat gcttttgaag aagtaatgac ttttcaggaa tctgatatcg aaaaatggct
    21721 gaaggaaaca ttgaactatt ttgctcagct atttgggcag ctgtcaatgc caaacgatag
    21781 agttgtccat ctctatcgtc gtgcacttta tcaaagcttt tctagtttcg gtatgaatcg
    21841 acagggacag gtcgtcggca gcaattgggg cagctatcct gctactaatc gaatttggaa
    21901 taaggatatg tattacgcat cattgccatt caccatgttt gaaccggagt tatgtcaaaa
    21961 aacggttcta tggtttgatc aatatggcgt taagtttcct ggttcaaaat ttacaggagg
    22021 tatcaatcat tctctgagta attcgttatc cagtgcgtta ctggctagtc tttattttga
    22081 acatactggg gatacaaaat tctttgagcg gtatccgcag gttctactga atgcttcaag
    22141 aattattgag gacatattag ctcaacgtcg gtcaggggaa ccaatgcttt ttccatctgt
    22201 ttggttatca gacgcctttg cacttggaaa gtatcatacc ggcagcaatc tttgtctttg
    22261 gaaggcctgt attggacttg cagaaatttt tgaagcccta gaacaactga gtcttagcaa
    22321 gcgatacaaa aacattgcct gtaaactaaa ggaatcaatt tgtaaacaaa tgactatcga
    22381 tggtcctttt ggtgagcaat ttttggaggg aatcggtgat gaggagaaaa caacctattc
    22441 agtgaagcac tatcaaaaac cgattcttga gcaagggtta atctttttaa gtgatgtgat
    22501 tgtggatgga aaaattaatt tattgatgca tgatggagaa gaatcagaca ctacgttgat
    22561 gcctttttat caatttttag ataataaaga tcatttatat cagaacacca tggccttttc
    22621 tgctagttct ttcaatccaa cctatagcga ggaaatcaaa ggcatcactt ggggattaga
    22681 atccggcgcc acttttcctg gctttatcac tgttttaatg agcgagctgc acaaccctca
    22741 agctttcgta acccgattag aagaactaat ttgtttggca gatttagacg gttcgtggtg
    22801 gtggtggccg tatcgcttag aggctcaaca aggagaagtc gtgcgagact ttggctgcgg
    22861 caaatgcggc tgggcatcgg gtctttttgt cagcctaatg atcagtcaat atttcggatt
    22921 gaaattcaaa aaaggagtat tgcaaattga tcctgttgat aatttaacgt tttcttggaa
    22981 gaatttgaag ttcggtcaag ccttcatttc gattgaatgt acgcaatccg agttgagtat
    23041 ttcaaatttg tcagaaaagc cattgaaaat aagtgatacg aaaaaaattc ttcacgtaga
    23101 aaagggaaaa acgattcaca ttcaaaggag aaaaagatga gtatactaaa aaatattacc
    23161 caatttaaaa aaatccagag tcgagcaatc actcccgaaa atccaacagg cgagaaaggt
    23221 aaagctgcga tgttgccaag cgagctagga ccaagccgca aaggtcgtgg cgcaattccg
    23281 ctgacttctg gtgaagaaac tgtgattgcg gatattactg gaacgggaat aattcgtcat
    23341 atgtggatga cgattcgtga tcgaacagaa tttggcagtt ttgttttacg tgatttaatc
    23401 ttaagaattt attgggatgg gtcggatagc cctgctgttg aaagtccttt aggggatttc
    23461 ttttgtaatg ggtttggaga gcgttgcgag gtgaactcgt tgccgatagt ggttaatcct
    23521 acagggggct ttaattccta tttcgaaatg cctttccaaa catccgctaa aattacgatc
    23581 gagaatttac atccgaagga tatgtcctca tttttttata cgataaatta cgctttggtg
    23641 gacgaattgg aagaaaatag cctatatttt cacgcaaatt ggcatcggga acgcattacc
    23701 aaattacagg aggattataa gttaattgat cgattaaagg gtcaaggcta ttacgtgggt
    23761 acttaccttg cgcttacctg cctagaacgt tattggtggg gcgaaggcga gtttaaattc
    23821 tacattgatg gggatagtga gtatccgacc gtaacctcaa caggttcaga agattatttt
    23881 ggcggggcgt gggcctttca tgagtacgat gagttcggac gtccacatgc gaaaaactat
    23941 tcaacagcct ttttagggta tccttaccaa tcaaatagag atgccacaag agatcgtttt
    24001 gaaacgggaa aattgaatgc cgttcatgcc tttggtgacg acgcaatgcc acgacatggt
    24061 ctttatcgtt ggcatcttca agaccccatc tgttttaccg aagatttatc ggtcacttta
    24121 caacaaatcg gcaacgatga tgttcgctta tttgagcggc aagacgatgt tgctagtgtc
    24181 gcctattggt attcaacaga agcttcagga aatactaaac gtcatttcac tgatcgagaa
    24241 cgcttgccgc gttaatgaag tgttttacac atattcgaag ggagaagcct atgatgatat
    24301 atggggcaat tgaagctgga gggactaaat ttatttgtgc agtcagtact gaggaattga
    24361 cgattttgga aagagtcagt tttcccacaa cgactccagt tgaaacaatg aagcaggtca
    24421 ttgatttttt taaacctttt aaggaacaat tggcaggaat cggggtcggc tgttttggtc
    24481 caattgatat tcaacgtaaa tcaaagaaat acggtcatat cacatcaaca ccgaaacttg
    24541 cttggcagaa tttcaatttt gtaggaacgc taaagcaaca tttcaatatt ccgattgaat
    24601 ggacaacaga tgttaatgcg gcttgctatg gtgaatatgt tgctggttct ggcaaagggt
    24661 tagcaggtgt tgcatacttc actgtgggaa caggtatcgg aggtggtgcc ttgttcaatg
    24721 ggactttcgt ggaagggttc agtcatccgg aaatggggca tatgttgatc aagcgacatc
    24781 caaaggacaa tttttcagga aactgtccct tccaccaaga ttgtttagag gggatggcag
    24841 caggaccagc gattgaaaaa cggttgggtg taaaaggcca gaatttattg gctgacgatt
    24901 cgttttggca gattgaagcc ttttaccttg cccaatgtgc atacaataca acactgatgt
    24961 tttcgccaga tagaattatt tttggcggtg gtgtaatgaa acaagaacac atgaagaaaa
    25021 aagttcagga taaatttgtt gagcttataa atggatatgt tgaaatacca ccaattgata
    25081 gctacattat tacgcccgaa ttaggtgata atgccggaat aatcggcgga ttagcattgg
    25141 ccagaaaagc ggtaagaaat aagcaaccat aattaatgaa tgtttcattg atactcatgt
    25201 caaaagtatt taaccgagta atcaaatgat caaatgaagt tggataaagc gaaaatgaat
    25261 tcaaataaaa aggctctaag tcaaatcgtg ttgttttcat taaatcactg aaaggaaatg
    25321 cttctgcgtt tccttttttc tttttgttgt gcgctgtata ccttgaggat attcactggt
    25381 ggcgtacact taaatagtaa tatattgtgt tacttt
//
LOCUS       DAZKLD010000026        24733 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.026, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000026 DAZKLD010000000
VERSION     DAZKLD010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24733
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.026"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..327
                     /locus_tag="ACG8KR_000885"
     CDS             <1..327
                     /locus_tag="ACG8KR_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0200280.1"
                     /translation="EERSCLYNDLLSIKDVYLCNKDTSFYTFRKIRRATKRCAECRKR
                     TFLYQTEHKGELSISQIHEQKRFKNHYRIQILFNGQEMEYAFLENLLQHLHNQHFSYN
                     SQAIYA"
     gene            complement(507..1802)
                     /gene="purB"
                     /locus_tag="ACG8KR_000886"
     CDS             complement(507..1802)
                     /gene="purB"
                     /locus_tag="ACG8KR_000886"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HFQ0200281.1"
                     /translation="MLERYTRPEMGNIWTEENKYRAWLEVEILADEAWAELGEIPQED
                     VKKIREHASFDIDRILEIEKLTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLIKQANDILREDLKRFTEIIGEKAKEHKYTVMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAKGVEAGKISGAVGTFANIPPFIEEYVCKQLGIRPQEISTQVLPRDL
                     HAEYFSAMALIATSIERFATEIRGLQKSETREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MAGLARVVRGHMVTAYENVTLWHERDISHSSAERIILPDTTILLNYMLNRFGNIVKNL
                     TVFPENMKRNMSATFGLIYSQRVMLKLIDKGMTREEAYDLVQPKTAYAWDNQVQFRPL
                     LEEDPTITSVLSKEEIDDAFDYHHHLKNVDVIFKRVGLE"
     gene            complement(1815..2939)
                     /gene="purK"
                     /locus_tag="ACG8KR_000887"
     CDS             complement(1815..2939)
                     /gene="purK"
                     /locus_tag="ACG8KR_000887"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HFQ0200282.1"
                     /translation="MVKPLLPGSTIGIVGGGQLGRMMAISAKEMGFKVGVLDPVSDCP
                     AAQVADWHIESVYDDTFALKELARRTDVITYEFENVSVEALNAILPMSFIPQGTDLLA
                     ITQDRLLEKSFLETNNIVIAPYATIVSPTDIQDAIDGIGYPCVLKTTRGGYDGKGQYV
                     LKSRADLAPAMDLLREGTCVLEAWIPFEKEISIMVAGNGQGDFTTFPVVENIHHNNIL
                     HETIAPAAIDQDVIEEAERIARVIAEAVSLSGVLGVEMFLTKTGGLYVNELAPRPHNS
                     GHYSIEACSMSQFDAHIRGICGWPLGEKVRLISNAVMVNVLGEQLLESYRLIENKPDW
                     QFHFYGKKQAKKGRKMGHITILTEDIQQTLEEIKQTNIWD"
     gene            complement(2932..3420)
                     /gene="purE"
                     /locus_tag="ACG8KR_000888"
     CDS             complement(2932..3420)
                     /gene="purE"
                     /locus_tag="ACG8KR_000888"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HFQ0200283.1"
                     /translation="MTAKISVIMGSTSDWETMKEACQILDEFGVSYEKKVVSAHRTPD
                     LMFEYAQNARKKGIKIIIAGAGGAAHLPGMVASKTTLPVIGVPVQSRTLNGLDSLLSI
                     VQMPGGVPVATTAIGKAGAINAGLLAIQMLSMYDLELEAKLAERRSMLAKTVIESSDQ
                     LG"
     gene            complement(3836..5143)
                     /locus_tag="ACG8KR_000889"
     CDS             complement(3836..5143)
                     /locus_tag="ACG8KR_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleobase:cation symporter-2 family protein"
                     /protein_id="HFQ0200284.1"
                     /translation="MEKETQNGKAAVLGLQHLLAMYAGAVAVPLLIGTGLGFNQEQMT
                     YLISIDIFMCGLATLLQLTVNRFFGIGLPVVLGCAIQAVAPLILIGTNEGVGAIYGSI
                     IASGIFVVLVSGVFSKIKKLFPPIVTGTVITVIGLTLIPVAIEKMGGGNATAADFGDK
                     TNLLLAFVTILLIVGVQMLAKGFIRSIAVLIGLVGGSILAAFMGMIDLSAIGDAPLFH
                     VPQPFYFGKPTFDVWSILLMIIISMVSMVESTGVYFALGDITGKNVGEEELKKGYRAE
                     GLAVILGGIFNTFPYTGFSQNVGLVQLSGIKTRKPIYFSAFFLIVLGLFPKIGALAQI
                     IPEPVLGGGMLVMFGMVAVQGIRMLLEVDFSNDKNLLIVAVSVGFGLGFNVTPTLFSQ
                     MPQTLQMFTGNGIVMSSITAIILNLIFNGLKKDEEILEEAFKK"
     gene            complement(5146..5727)
                     /locus_tag="ACG8KR_000890"
     CDS             complement(5146..5727)
                     /locus_tag="ACG8KR_000890"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0043101 - purine-containing compound
                     salvage [Evidence IEA];
                     GO_process: GO:0046110 - xanthine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HFQ0200285.1"
                     /translation="MKELVERIQKDGRILGEGVLKVDSFVTHQVDPVLMEQMGKRFAE
                     VFKEQGITKVVTIEASGIAPALFAARELDVPMIFARKAKSLTMDEELLTASVYSFTKQ
                     VTSTISISRKFLSSEDKVLIIDDFLANGQAAKGLIELCQQAGAQVEGIGIVIEKSFQD
                     GRQLLEEMGLRVVSLARIASLTNGQVEFLEEDA"
     regulatory      complement(5843..5940)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            complement(6299..7591)
                     /locus_tag="ACG8KR_000891"
     CDS             complement(6299..7591)
                     /locus_tag="ACG8KR_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFQ0200286.1"
                     /translation="MKKKYVQVGLGGRARFFYEAIASTYHQTCELVGFCDVSQTRMDY
                     ANRILVNKYGQKKVKTCMPSQFEQLLDDVRPAEVIITSVDRTHHDYIIRAMKKGYDVI
                     CEKPLTIDEEKAQEIITVQKQTGRSLRVTFNYRYAPYHTKIKELIESDVIGDVFSIHF
                     EWLLNTQHGADYFRRWHRNKHNSGGLLVHKSTHHFDLVNFWLNSRPQKVFAFGGLNFY
                     GMHNAEKRGVEIFYSRCYGSELAKADPFAIDLSANEELREMYLEAEKDSGYIRDRSVF
                     SDDISIEDTLGVTVKYETGAILTYSLNAYMPWEGFNVSFNGSKGRIEMKVIEKSYINA
                     GGDKKDEGAAVLQSIHVYPMFGEPYEVIVEQEAGGHGGGDPRLLDDLFGEEKEEDPWN
                     RAATHVDGILSILTGIAANHSIASGKAVVIDELVSLKE"
     gene            complement(7588..8211)
                     /locus_tag="ACG8KR_000892"
     CDS             complement(7588..8211)
                     /locus_tag="ACG8KR_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0200287.1"
                     /translation="MKKPIEEMFLYKLNYYVCRIFLTSLISNALFALVLGCLFTLGLV
                     LSARVVYLIYFLMMYPSYYLSVHYLAEKKVCWRKGKIKRMCFIITLFQLFVIIFAVDW
                     YLLVGKIGAWYLIPFLLCLLLFVSHSFFSTLIDICKEDKADSLVYLIKRNIWLLSKNV
                     KGFLWYLCLLYGLFWCIQHDFLLTALLYPGLPFVTVFCLKEKENDFK"
     gene            complement(8214..9050)
                     /locus_tag="ACG8KR_000893"
     CDS             complement(8214..9050)
                     /locus_tag="ACG8KR_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0200288.1"
                     /translation="MNNTVTKWFQYGFILLLGLVMIYPVLWMIAGSFKTDQEIMSGSL
                     NLIPETFQWENYAKGWAGFAGISFFTFFRNSFVISAVSTIGTVLSSTCIAYALSRIDF
                     KGKRFWFVVMLVTMMIPAQVILIPQFIIYNRLNLVGTYVPLILPHFFGQAFFIYQIMQ
                     FMVSIPKELDESAIIDGCSKYSVFTKIIFPLLKPSIITTIIIQFYWKWDDFMGPLIYL
                     NKPRSYTVSIAIKLFADAGSSTTYASMFAMSTLSLLPVFLIFLFFNKYLVQGISTSGL
                     KG"
     gene            complement(9067..9954)
                     /locus_tag="ACG8KR_000894"
     CDS             complement(9067..9954)
                     /locus_tag="ACG8KR_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0200289.1"
                     /translation="MKKEKLKRFLDQDNVVGYVFASPFILGFLLITVIPMILSLYYSF
                     TDYRLGNPVSWIGLDNYIKMLSDARFLNSIRVTFLYVFASVPVKLIFGLLIAYLLTRN
                     IKGTTFFRSLYYVPSLIGGSISIALVWRELFSKNGLVNIFLERLGAPALSWFSDQKLV
                     MIPLVLMSAWQFGSAMIIFAAGIKEIPGSYYEAAEIDGANKWQTFYKITLPLLSPIIL
                     YNLVMQTIAAFMTFTQAFIITQGGPNDATNFYALYVYNQAFSFNSMGYASAISWIMLL
                     IMGAVTFLIFKSSKKWVVV"
     gene            complement(9970..11253)
                     /locus_tag="ACG8KR_000895"
     CDS             complement(9970..11253)
                     /locus_tag="ACG8KR_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFQ0200290.1"
                     /translation="MKKRMRIILALSVMLFFLTACGQEDDKKITLNMAWWGSQVRHDA
                     TVKVIELYEEKNPHVTIDYEFYDYEGYYTKLGTLAASDDIWDIFQLNNQFPQYINQIE
                     PLDEYVEQGIIDISNADESYLATTTYDGQLVGLSNGVNSFAIAYNKNIFEQLNIPEPA
                     SNWTWDEFEQISYQITEELGIYAISRFEDFIAGCIIQIPQVEKGLNFYNREDLSNSLG
                     FENPDYLTDFFQLRKNLVDKGAHPEPGGGAASTTDLSFQAVRDREAAMVFLSSNQLTE
                     ILSGAPEDMEIQLINPPRRKADGESGIPLRSSQMLSMAKSSAHKEEAAKFIDFFQNSE
                     EANEILKGERGVPIMSNIREQLARADDATLQATFDYLEMISDMDNGEVNVFDSALNPE
                     IQDQYNLLVEKVIYEEVTAEEAAESLYEFASNILG"
     gene            complement(11936..12619)
                     /locus_tag="ACG8KR_000896"
     CDS             complement(11936..12619)
                     /locus_tag="ACG8KR_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFQ0200291.1"
                     /translation="MAEQFTPEIMTELRKDIVRVPEIILEASGIKIFGKLIRSIIFTT
                     DIAIIRNTNANAVIAVYPFTPHPAITKSIIEAADIPVFSGVGGGLTQGARSSYMSMFA
                     EAQGSLGVVLNGPTPLETVKEVCRVVDIPVISTVTSKYTEIDEKLAAGISIINISAGK
                     ETAEAVRHFRKKYPELPIIATGGPTEESIAETIQAGANAITYTPPSSGELFSRKMEKY
                     RETEKKNHE"
     gene            complement(12637..13254)
                     /locus_tag="ACG8KR_000897"
     CDS             complement(12637..13254)
                     /locus_tag="ACG8KR_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HFQ0200292.1"
                     /translation="MAKKKWKKQKRKNIRWPAVVAGVAVILLAFVFSLKNLSSPFTDN
                     QQENEAQSHQQFIERLVPHAQELQDGYGVLPSIILGQAILESNWGKSTLASKYNNLFG
                     IKAYGDQKKVSLETKEFVNEEWITIQGDFKVYDSWEQSMDDHTQLFVQGVDWNPALYE
                     KVITATNYQEAAQALQDAGYATDPGYAQKIIQVIETYQLNQYDRR"
     gene            complement(13264..14088)
                     /locus_tag="ACG8KR_000898"
     CDS             complement(13264..14088)
                     /locus_tag="ACG8KR_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HFQ0200293.1"
                     /translation="MNKFLGFGAVVIMIAAMGYVIFSDDDSHVQAGNQAVERTSTKTT
                     ASKEKNSQTTSSKVKDKETKDRTSKQLPDVKTDDWKLVLVGPDHKLEKEIDESTQLAS
                     LSNGYLIDKRIAPEYEAFAKAAEAAGFPLVMVSAYRSVSSQQQVFEQNVQDVMSRQGV
                     SEEEATKITKETITEPGYSEHHTGLAVDVVDQNWYNSYPSTVLDASYGDQPGAKWIAD
                     NAAQYGFIVRYPKGHEDITKITYEPWHLRYVGKENAEYITKHQLTLEEYLNELNEK"
     gene            complement(14081..14641)
                     /locus_tag="ACG8KR_000899"
     CDS             complement(14081..14641)
                     /locus_tag="ACG8KR_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0200294.1"
                     /translation="MHQVYIDLIMSVLLEQFETEKNFLDEYLLISPEQWQKWKDGEKN
                     LPSEAMQKVKNLFTDYEWMLTQKILRQTIIFPEKRNVAVAEYKQIKTRIAQKWLSSKA
                     AKVELIPLKNNQQAGRYLDLKVSIQYDEWGYDDILNFRLPADVQKQIEGEKIELLDWV
                     NENLEETYVHEANGEREEQRKDFSSE"
     gene            14815..15771
                     /locus_tag="ACG8KR_000900"
     CDS             14815..15771
                     /locus_tag="ACG8KR_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFQ0200295.1"
                     /translation="MKKYQWGIVGLGTIAHEFAESFNQETSELTAVASRTSEKAENFA
                     HRYNIPKAYGSYQEMLDDAEIDIVYIAVPNRQHIDHILAALEAGKHVLCEKAITMNKK
                     ELADAMRLAEEKNLILAEAMTIFNMPLYQQLRSIMDTGKLGALKMIQAPFGSYKEPDP
                     KNRFFNPELAGGALLDIGTYAVSFARFFLSSQPEVVASTMVPFETGVDEQSVTILRNK
                     ENELAAVSLTFQAKMPKVGIVAFENGYVTIADYPRADRAEILFTDGTKEFIESGNTSE
                     AMNYEIKNMVHMIEGDLPNRSLFLTKDVIEILDQMQVLWKNM"
     gene            complement(16076..17032)
                     /locus_tag="ACG8KR_000901"
     CDS             complement(16076..17032)
                     /locus_tag="ACG8KR_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HFQ0200296.1"
                     /translation="MRIGMRTVKTVISVFFSMVTAGSLSLLYWPAAGIIALLSVGNTK
                     RSTLRTGMNRIAAFILATATALFSFFAVGYTIFGFSLFLFLFIPLASRFKLTEGIVVN
                     SVLVTHYLVEENMSWQLIGNEAGLMAIGLVFALLANVYMPDRTKQLKENQIQIEKEFR
                     NILRQMAEFLLSENKEDVQIKCEHLLTFIRESQEDAREHQENYWLRQPLYYETYFSMR
                     RAQANVIKDMLENLERIQQPAYYGKHIYGLLIYTAETFSESNDGRQILTRIEEVYVLY
                     RQMPLPTSRPEFEDRAELFQFLQSFKSFIEIKAEFSQQKIQK"
     gene            17136..17738
                     /locus_tag="ACG8KR_000902"
     CDS             17136..17738
                     /locus_tag="ACG8KR_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFQ0200297.1"
                     /translation="MQFGRFRLGMRTIKSALAVFLCILFFHLTQRGIPMIAALSAVFS
                     LRQDLTTTVSFGKSRIIGNTLGGTLAIIYFFVKSYFHNDFLVELILLPILVIIVIVVS
                     DGINNNSGIISAIATLLLIALSIPQGESALYAIQRVLDTFIGTFIAIGINFFLRPPET
                     EKKEELSEDLVELQKKERLLRTNLQEIEAKIQKQKKNSED"
     gene            complement(17826..19325)
                     /locus_tag="ACG8KR_000903"
     CDS             complement(17826..19325)
                     /locus_tag="ACG8KR_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HFQ0200298.1"
                     /translation="MEIPVVFIAIAIAALILLMLLIVFVAKYQTAKPDEALIISGSYL
                     GSKNVHIDESNNKIKIVRGGGAFVLPVFQRSNRISLLSSKLDVSTPEVYTEQGVPVMC
                     DGTSIIKIGSSVEEIATAAEQFLGKTREELENEAREVLEGHLRSILGSMTVEEIYQNR
                     DKFSQSVQEVASVDLAKMGLIIVSFTIKEVRDKNGYLDSLGKPRIAQVKRDADIAEAE
                     ALKETRIKKAQAEKESQAAELQRQTEIAESLKEKELKLATYKQEQDVAKAKADQAYNL
                     ESARAQQQVIEQEMQIKIVERQKQIELEEKEITRREKQYDSEVKKKADADRYAKEQEA
                     QAQKVKEVTEAEAERFRVEALAEAEANKTRLAGQAEAEAALAKGKAEAEAKQKIANAF
                     KEYGEAAVLSMVIDMLPQLMREAAQPLGNIEKISVVDTGSSSGETSGANRVTNYATNL
                     LSSTQETLKATTGLDLKEIVENLSTRGTTSQVNMYEAPKEAEQNSMKEE"
     gene            complement(19341..19865)
                     /locus_tag="ACG8KR_000904"
     CDS             complement(19341..19865)
                     /locus_tag="ACG8KR_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HFQ0200299.1"
                     /translation="MIGGIALETIYWYTLLICAGLAVLLIIFGDVFDFDGPLDPMLII
                     PWLTFTSLFGFLGERLTDQSSLFLFIASGGISIVLVFLLNFYILMPMKHAESTMSSSE
                     KTLEGQVAVVITPIPVRGMGEIQLKSVTGSISRPACFYSPQEQTAERGTKVLIIEIKE
                     RICYVMPYESMIKA"
     gene            20163..20912
                     /locus_tag="ACG8KR_000905"
     CDS             20163..20912
                     /locus_tag="ACG8KR_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HFQ0200300.1"
                     /translation="MNRSSVSDKISSIAKIVANDKLIQKKLQTGDPTHAIQGITNEIM
                     IASNLDFIVVMDPEGIRYSHADPSYIGLPFSSHEDVKKVIGGQEFFSEKEGMLGFGFR
                     YFVPIYYDQEIIGAVCVGLTNNTIDNQVKDGQKMIVFSLIIGLSVGIIGAVFLAQKMK
                     KVLLGLEPQEIAEQLSEKTIIENEVDEGILAISSDKTILLINTPAKNIFYQTEQLLDF
                     EVGKKSVIIYMTFCLKRRLSIKRPFGLNPCI"
     gene            20903..21688
                     /locus_tag="ACG8KR_000906"
     CDS             20903..21688
                     /locus_tag="ACG8KR_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFQ0200301.1"
                     /translation="MYLNGIETISSVTPLFSNDRFLGAVATLRNRSEVMKLMEELGGT
                     KQYINSMRAQTHEFMNKLHVISGLIDLKKYEEVTHFIQQLNQNYKKEIGQVSSLIKVP
                     AIAGFLLGKINEAKEQSVEMLLESDSFIPKMEMDDSIHSLLQVLGNLLDNAKEAVLQK
                     HERNGQVRIHLNYDTEAEVFIVTVCDDGVGISEEISEKIFTLGFSTKGINRGFGLNLI
                     HSIVKRHGGFLEFSKQTNGGTIVYLELPKKEDQNDDKCPDNRR"
     gene            21657..22373
                     /locus_tag="ACG8KR_000907"
     CDS             21657..22373
                     /locus_tag="ACG8KR_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFQ0200302.1"
                     /translation="MMTNVLIIEDDPMVAELNRTFVESIPNMNVIESFRNVEEGKKYF
                     RQKNADLLLLDVYMPGESGIHFLSWLRQQGNDIPVILITAADDIETIKNVIRYGALDY
                     LIKPFTLQRFQLAIEKYLAFSQTIQTTEKADQKLIDQVFNHTTPIHEIPSFIDLPKGL
                     SKLTLKKISAFILKEDTIFSTKELAQKSSLSRISVKKYLNFLVEQDILNERIKYLEIG
                     RPLTCYELTKNGRKKLTDYC"
     gene            complement(22475..23494)
                     /locus_tag="ACG8KR_000908"
     CDS             complement(22475..23494)
                     /locus_tag="ACG8KR_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HFQ0200303.1"
                     /translation="MRIACYGVRPNEIDFFEKLNIYHYDLSLYEELLTHDNIVSAKDH
                     DAVLLRGNCIADETNLAKMQEYGIRYVFTRTVGVNHIDLQAAADFGMTVARVPSYSPN
                     AIAELSLTFAMMLLRNTAYTTIRTSFKDFRVDEQMFSREIRNCTVGIIGTGRIGLTEA
                     VLFKGLGAKVIGYDIHQTEAAKKVLTFHSLEELLAKSDIVSIHVPHIPGENDQMINEG
                     FLAQMKKGSILINTARGELQDNEAILRALKSNHLAGFATDVFANETEVFFRSFKPWET
                     IPDPAIQQLVEMYPRVLVTPHVGSNTDEALSNMISTSFENFREIIETGKTKNEVSLPK
                     ARQLR"
     gene            complement(23513..24514)
                     /locus_tag="ACG8KR_000909"
     CDS             complement(23513..24514)
                     /locus_tag="ACG8KR_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HFQ0200304.1"
                     /translation="MTSTVFIILLGFVCRKKGIFSESVGKILSKVVLTVALPALAFRA
                     FMQDINTELLMQGINVLIWGLVIYIILILVSKPFFLSYKGDKQTTLRVLTIFGSTTFF
                     GTPIVSAIYGPVGVMYSSIFNIGYRIFLYSYGYIKMSGLKMEAKNIKAMFVNPIIIAT
                     FAGLFIWLFQGYLPQVSVTGAEGEISQVAFLRIDQTAPWLFQPMDYLAGLSSPLAWLA
                     IGATLGEVSFKKAASEKTSWYYSLVKVVFVPALNIVILAVLTATNILPVNFEALATIV
                     IMMATPAATVAAAYAISFDREALLASNASLLSTIFAVVLTPVWIVVLQMIHGIGLFS"
BASE COUNT         7350 a         5312 c         4304 g         7767 t
ORIGIN      
        1 gaagaacgtt cctgtctata taatgatttg ctgtcgatca aagacgttta tttatgcaat
       61 aaagatacta gtttttatac attccgaaaa atccgccgag caaccaagcg ctgtgctgaa
      121 tgccgtaaaa ggacttttct ttatcagaca gaacacaaag gcgaactttc aatcagtcag
      181 attcatgaac agaagcgttt caaaaatcat tatcgtatcc agatcttatt caatggacaa
      241 gagatggaat acgcttttct cgaaaacttg ctgcaacatc tccataacca gcattttagt
      301 tataatagcc aagccattta cgcttaattc ctctattttt tgtatgtaaa gaggcacggt
      361 aaagaggtag ccgcttttaa aatcaggttc ctattattga taagaaaata taataaacta
      421 aaagaagcag aaccgctaac ggatctgctt cttttttggt tatttttgct attctatttt
      481 aattgaattt tgcaactcac tcttctttat tccaaaccta ctcgtttgaa tatcacgtca
      541 acgtttttca aatgatgatg ataatcaaag gcatcgtcta tttcttcttt agataatacc
      601 gatgtgatgg ttggatcttc ttctaacagc gggcggaact gcacttggtt gtcccaagcg
      661 tacgcagttt ttggctgaac aagatcataa gcttcttctc ttgtcattcc cttatcgatc
      721 aatttcagca taacgcgctg gctataaatc aaaccaaatg ttgcagacat attccgcttc
      781 atgttttcag gaaagaccgt tagattttta acgatattgc caaaacgatt taacatgtaa
      841 ttcaataaaa tcgtggtatc aggcaagatg attcgttctg ctgaagaatg agaaatatct
      901 ctttcatgcc ataatgtgac attttcatag gcagtgacca tgtgtccgcg caccactctg
      961 gctagaccag ccatattttc tgaaccgatc ggattccttt tgtgcggcat agcagaagaa
     1021 cctttttgac ctttcgcaaa aaattcttct acttcacgtg tttcggattt ttgcaatccg
     1081 cgtatctctg ttgcaaaacg ttcaatactt gtagcaataa gcgccatcgc tgagaaatat
     1141 tcagcatgta gatcccgagg gagaacttga gtagaaattt cttgtggacg gatccccaat
     1201 tgtttacata catactcttc aataaatggc gggatatttg cgaacgttcc cactgctcct
     1261 gagatttttc ctgcttctac tccttttgcc gcatgttcaa atcgttcgat gttccgtttc
     1321 atttctgaat accatgtggc aagtttcaat ccaaaagtag ttggttcagc atggacaccg
     1381 tgtgtccgac ccatcatcac tgtatatttg tgttcttttg ctttttcgcc aatgatttcc
     1441 gtaaaacgtt tcaagtcttc ccgtaaaata tcatttgctt gcttgatgag atagccataa
     1501 gcagtatcta cgacatccgt acttgttaga ccataatgga cccattttct ttcttctccc
     1561 aatgtttcag aaaccgctcg tgtaaacgca acgacatcat ggcgtgttaa tttttcgatt
     1621 tccaagatcc gatcgatatc aaatgatgca tgctcacgaa tttttttcac atcttcttgc
     1681 gggatttcac caagttctgc ccaagcttca tctgctaaaa tctcgacttc gagccacgca
     1741 cggtatttgt tctcttccgt ccagatattc cccatttcag ggcgtgtata acgttctaac
     1801 atgttttttc tcctctaatc ccaaatattc gtttgtttga tttcttccag cgtttgctgg
     1861 atgtcttctg tcaagatagt gatatgtccc atcttgcgac ctttttttgc ttgttttttt
     1921 ccatagaaat ggaattgcca gtctggctta ttctcaatca agcgatagct ttccagcagt
     1981 tgttctccca aaacattgac cattactgca ttgcttatca aacgtacttt ttctcctaat
     2041 ggccaaccac aaatacctcg aatatgggca tcgaactgac tcatagaaca agcttcaata
     2101 gaataatgtc cagaattgtg aggacgagga gctaattcat tcacatatag cccgcctgtt
     2161 tttgttaaaa acatttctac ccctaaaaca cctgaaaggc tcactgcttc tgcaatcaca
     2221 cgggcaatac gctcagcctc ctcgatcaca tcctgatcaa tcgcggctgg agcaatcgtt
     2281 tcatgaagaa tattgttgtg gtggatgttt tccacgaccg gaaaagtcgt gaaatctcct
     2341 tgaccatttc ctgccaccat gatcgaaatc tctttttcaa acgggatcca tgcttctaaa
     2401 acacaggttc cttctcttaa aaggtccatg gcaggagcta gatccgcccg acttttcaaa
     2461 acatactgcc ccttgccatc atagccgcct cgagtggttt tcaaaacaca aggatagccg
     2521 atcccgtcaa tcgcatcttg gatatccgtc ggactgacga tcgttgcata aggagcaatc
     2581 acgatgttat tcgtttccaa aaaagatttt tctaacaggc ggtcttgcgt aatcgcaagc
     2641 aagtctgtcc cttgaggaat aaatgacatg ggtaaaatcg catttaacgc ctctacactg
     2701 acgttttcga attcataggt aatgacatct gttcttctgg caagctcttt cagcgcaaag
     2761 gtgtcatcat agacactttc aatatgccaa tctgcaactt gtgcggctgg acaatcgctc
     2821 acaggatcca gcacacctac tttaaagccc atttcttttg cactaatcgc catcatacga
     2881 cctaattgac cgccaccgac gatcccgatc gttgaaccag gcaacaaagg tttaaccaag
     2941 ttgatcacta ctttctataa cagttttagc caacattgaa cgtctttcag ccaatttagc
     3001 ctccaattct aaatcataca tggaaagcat ctgtatcgca agtagtccag cattaattgc
     3061 tcccgctttt ccgattgctg tagtagctac aggtactcct ccaggcattt ggacaatcga
     3121 aagaagcgaa tccaaaccat ttaatgttcg tgattggacc ggtacaccga taaccggcaa
     3181 agttgttttt gatgcgacca tacccggcag atgcgcggct ccccctgctc ctgcaataat
     3241 gattttgatt cctttttttc gagcgttttg tgcatattca aacatcaaat ctggggttcg
     3301 atgagcagaa acgacctttt tttcataaga cacaccgaat tcatccaata tttgacatgc
     3361 ttctttcatt gtttcccaat ctgaagtact ccccataatc acagagattt ttgctgtcat
     3421 tgtttttcct cccagttttt ctacattcat attttaacaa tcactttttt tgatgtcaaa
     3481 gaaatttcga atgttaacaa agaaaaacat tttattgttc gtgtttatta aagataaaaa
     3541 aaaggaatgt gacaaaagtt aggtcacatt cctttttatg gataaacggt gttctcctgc
     3601 caactcctcg gtcacaagga acaatattca acaaacatga gaagcttttt tttgaaattg
     3661 ttccttgtga ctcggagatg aacggcaggc tcacgacctc ttccttcaac ggtgttcaat
     3721 cagctgaccc tcggtattag ctcaaacaaa cggcaaaata cgaggaacta tttttccgtt
     3781 tgtttctcta acaatcaggt caaaacgctg atttcacaac ctttcttttg ctctattact
     3841 tcttgaaggc ttcttccaat atttcttcat cttttttcaa tccattaaag atcaaattca
     3901 atatgatagc cgtaatgcta ctcattacga tgccatttcc agtgaacatt tgcaatgttt
     3961 gcggcatttg actgaataat gtcggcgtaa cattgaaacc cagcccaaat ccaacagaaa
     4021 cagcaacaat caatagattt ttatcattgc tgaaatcaac ttccaacagc atacggattc
     4081 cttggactgc caccatcccg aacatgacta acattcctcc accaagtacc ggctcaggga
     4141 tgatttgtgc taacgcaccg attttaggga ataatcctaa aacaatcaag aagaatgcag
     4201 agaaatagat gggttttctt gttttgattc ccgaaagttg aaccaatccg acattttgag
     4261 aaaagccagt ataagggaat gtattgaaaa taccgcctaa gataacggca agtccttctg
     4321 cacgatatcc ctttttcaat tcttcttctc caacattttt cccagtaatg tcaccaagtg
     4381 caaaatatac acctgttgat tctaccatgc tgaccatgga aatgatgatc attagtaaaa
     4441 tcgaccatac gtcgaaagtt ggtttaccaa aataaaaggg ttgtggaaca tggaacaacg
     4501 gcgcatctcc aatcgcgctc agatcaatca ttcccatgaa agcagctaag atgcttcctc
     4561 cgactaaacc aatcaatacc gcgattgaac gaatgaaccc ttttgccagc atttgcacgc
     4621 ctacaattag gagaatcgtc acaaatgcaa gtagcagatt tgttttgtca ccaaaatcag
     4681 ctgctgttgc attacctcca cccattttct cgattgctac aggaatcaaa gtcaaaccaa
     4741 taacggtgat aaccgttcct gttactatag gagggaatag ttttttaatt ttagaaaaga
     4801 caccagaaac taacacaaca aagatcccag aagcgataat agatccgtag atagctccta
     4861 ctccttcatt ggttccgatc aagatcagtg gagctactgc ttggatcgca catcctaaaa
     4921 cgacaggcaa gccaattccg aaaaatcggt taactgtcaa ttgcaataat gtcgctaatc
     4981 cacacataaa gatatcaatt gaaatcaaat aagtcatctg ttcttgatta aagcctagtc
     5041 ctgtaccgat caaaagaggc accgctactg ctcctgcata catcgctagc agatgctgca
     5101 gccctaaaac cgcagctttt ccattttgtg tttctttttc cacgattatg cgtcctcctc
     5161 aagaaactca acttgtccat tcgtcaagga agcaatacgc gcaagagaaa cgactctcaa
     5221 tcccatttct tcaagtaact gacgtccatc ttggaaagac ttttcgatga cgatcccgat
     5281 tccttctact tgcgctccag cttgctggca taattcgatc aatccttttg ccgcttgtcc
     5341 gtttgccaag aaatcgtcga taatcaatac tttgtcttct gaagataaaa atttacgaga
     5401 aatagaaatc gtacttgtca cttgtttcgt aaaagaataa acagaagctg tcaataattc
     5461 ttcatccatc gtcaaacttt ttgcttttct agcgaagatc attggaacat ctaattctct
     5521 tgcagcaaat aaagctggtg cgatcccaga agcttcaatg gtcacaactt tcgtgattcc
     5581 ttgttcttta aatacttctg caaaccgttt tcccatttgt tccatcaata cagggtctac
     5641 ttggtgagtg acgaagctgt cgacttttaa tacgccctcg cctaatatac gtccgtcttt
     5701 ttgaatacgt tcaactaatt ctttcacttt atttgctcct ttatcattta ttaataccga
     5761 aataaccgct tttgtgcaaa aacctactat gtttctgtta aagaaaaaca ccctccactt
     5821 tatcataaga aaagaagggt gaaatagcca atcttttact tatagtccag catttacggt
     5881 actggctaga aactgtcatc catattatga cgatatataa gtaggtatgt aaaactaata
     5941 atagtgtaaa ttcattttct tgcaactgtt ttgcacgtga aggcagtttg ccatatttga
     6001 ataaaaaatg ttgtttcaaa tattgaaaac agtctaacac tactttctga aactgacaac
     6061 gtggtttact tatttttttg taaaatctaa tgatttttaa tgcattattc ctttttgttc
     6121 gggtttaaat agttattttt attttctcct atctaaaaaa gcagcccaca taaaaagaag
     6181 gcatgacaaa aagcatgtca tagccccttt ttccgaataa atagtgttca aaagacagcc
     6241 tatcggcaat aaggcaagat tttataaact acgggaagta atttccgtaa atctattctt
     6301 attcttttaa gctgacaagc tcgtcaatca ctactgcttt tcctgaagca atagaatgat
     6361 ttgctgcgat tcctgtcaaa atcgacaaaa taccatctac gtgagttgct gcccgattcc
     6421 acggatcttc ttctttttct tcaccaaata gatcatctaa taaacgcgga tctcctccgc
     6481 catgtccgcc cgcttcttgt tcgacgatta cctcatatgg ctcaccgaac atcggataga
     6541 catggatgga ctgaagaacg gctgctcctt catccttttt atctccacct gcattgatgt
     6601 aagacttttc gatcactttc atttcgatcc gtcctttgct gccattgaaa gaaacattga
     6661 atccttccca cggcatatac gcgtttagcg aatacgtcaa aatcgcacca gtttcatatt
     6721 tgaccgttac gcctagtgta tcttcaatag agatatcatc ggaaaataca ctgcggtcac
     6781 gaatatatcc actgtctttt tctgcttcta aatacatttc tcgcaattcc tcattggctg
     6841 acaggtctat agcaaatgga tcagctttag ctagttcgct tccatagcat cgactgtaaa
     6901 atatttctac tccgcgtttc tccgcattat gcatcccata aaagtttaat ccaccgaatg
     6961 caaatacctt ttggggacgg ctgttcaacc aaaaattcac tagatcaaaa tgatgggtag
     7021 atttatgaac aagaagcccg ccgctattgt gcttatttcg atgccatctt ctgaaataat
     7081 ctgccccgtg ttgcgtattt aacagccatt cgaagtggat agaaaacaca tcaccaatca
     7141 catcactttc tatcagttcc tttatcttcg tgtgatacgg tgcataacga taattaaaag
     7201 tgactcgcag gcttcttccc gtctgttttt ggaccgttat tatttcttga gctttttcct
     7261 catcgatagt cagaggtttt tcgcaaataa catcatatcc ttttttcatt gcacgaatga
     7321 tataatcatg atgcgttcga tctactgacg taataatgac ttcagccggc cgtacgtcat
     7381 ccagtagttg ctcaaattgt gatggcatac atgtctttac ttttttctgt ccatacttgt
     7441 tcacaagtat ccgatttgca taatccatcc ttgtttgaga tacatcacaa aaaccgacta
     7501 gttcacaagt ttgatgatac gtagaggcaa ttgcctcata aaaaaaacgc gctctcccac
     7561 caagtccgac ttgtacatat tttttcttca tttaaagtca ttctcctttt cttttaagca
     7621 aaatactgtg acaaatggca gccctggata cagtaatgca gtgagtaaaa aatcatgctg
     7681 tatacaccag aacagtccat acaaaaggca taaataccac agaaacccct tgacgttttt
     7741 tgacaacagc cagatattcc ttttgatcaa ataaacaaga ctatctgctt tatcttcttt
     7801 gcagatatca attagcgtac taaaaaaact atgggataca aaaagcaaca gacacaatag
     7861 aaaaggaatc agataccaag ctccaatctt tcccactaat aaataccaat caactgcaaa
     7921 aataatgaca aacagctgaa acaacgtaat gatgaaacac atccgtttga tttttccttt
     7981 cctccaacat accttttttt ctgccaagta atggacagat agataataag aaggatacat
     8041 catcaaaaaa taaatcagat agacaacacg agcagacagc actaatccta aagtgaacaa
     8101 acatcctaga actaaagcga aaagagcatt actgataaga ctggttaaaa aaatccgaca
     8161 gacatagtag ttgagcttgt aaagaaacat ctcttctatt ggctttttca tattcagccc
     8221 ttcagaccgc ttgtactgat cccctggaca agatatttat tgaagaataa gaaaatcaga
     8281 aaaaccggta gcagcgacaa cgtagacatc gcaaacatac tcgcgtaagt cgtactagat
     8341 ccagcgtctg caaataattt gatggcaatc gataccgtgt aagatctcgg cttgtttaaa
     8401 taaatcaatg gtcccatgaa gtcgtcccat ttccaataaa actggatgat gatcgtcgtg
     8461 atgatcgaag gtttcagcaa aggaaaaata attttggtaa agacactata cttgctgcat
     8521 ccgtcaataa tcgctgattc atctaactct ttggggatgc tgaccatgaa ttgcatgatc
     8581 tggtaaatga aaaatgcttg tccaaaaaaa tgtggtaaaa tcagcggcac atacgtccca
     8641 accaaattca gccgattgta aataataaac tgcggaatca aaatcacttg tgcaggaatc
     8701 atcatcgtta ctaacatgac gacgaaccag aatctttttc ctttgaaatc gattctcgat
     8761 aatgcgtaag caatacaagt cgagctcaga actgtcccta tcgtcgaaac tgcagagatg
     8821 acaaacgaat tcctaaagaa agtaaaaaat gaaattccag caaaccccgc ccatcctttt
     8881 gcgtaatttt cccattgaaa agtctctgga atcaaattca gcgaaccact cataatctct
     8941 tgatccgttt taaaagaacc tgcgatcatc cataagaccg gataaatcat gactagacct
     9001 aatagcaaaa taaaaccgta ttgaaaccat ttcgttactg tattattcat ttcttcctcc
     9061 tctcattcac acaacgaccc atttttttga tgatttgaaa atcagaaagg tgacagctcc
     9121 cataatcaac agcatgatcc acgaaatagc actagcatac cccatagaat taaaagaaaa
     9181 agcttggtta tacacataaa gagcataaaa gttcgtggca tcattcggac caccttgggt
     9241 aatgataaat gcctgcgtaa aagtcataaa cgccgcgatt gtctgcataa ccaagttata
     9301 caaaatgatc ggcgaaagaa gaggtaacgt gatcttgtaa aacgtctgcc atttgttcgc
     9361 tccatcgatc tctgccgctt cataatacga gcctgggatc tctttgatac ctgcagcaaa
     9421 gatgatcatt gccgaaccga attgccaagc agacatcaaa acaagaggga tcatgaccag
     9481 tttttgatcg ctaaaccagg aaagcgcagg tgcgcctaat cgttccagga aaatattcac
     9541 taacccgttt ttagaaaaca actcccgcca aactagagcg atagaaatac tgccaccgat
     9601 caaactaggc acataataca aagaacgaaa gaaagtcgtt cctttgatat tcctcgttaa
     9661 aagataagca atcaatagac caaagatcag tttcacaggt acagatgcaa atacatagag
     9721 aaaagtgact cggatggagt tcaaaaaacg ggcatcactc agcatcttta tataattatc
     9781 caagccgatc catgagactg gatttcccaa acgataatcg gtgaacgaat aataaagtga
     9841 caagatcatc ggtatcactg ttatcaatag aaatcctagg atgaatggag aagcaaatac
     9901 gtaaccgacc acattgtctt gatccaagaa ccgttttagt ttttcttttt tcatgccttc
     9961 ctcctgctgc tatcctagaa tgtttgacgc aaactcataa agactctctg ctgcttcttc
    10021 tgctgttact tcttcataaa tgactttttc aactaaaaga ttgtattggt cttgaatttc
    10081 cggattcaaa gcactatcaa agacattcac ttcgccgttg tccatatcgg aaatcatttc
    10141 taaataatca aatgtcgctt gcaaagttgc atcatctgca cgagcgagct gttctcgaat
    10201 attcgacatg attggtacac cacgttctcc ttttaaaatt tcattggctt cttcactgtt
    10261 ttgaaagaaa tcaataaact tagcagcttc ttctttatgg gctgagcttt ttgccatact
    10321 cagcatttgc gaagaacgta aagggatacc agattctcca tctgcttttc tgcgaggtgg
    10381 attaatcaac tgaatctcca tatcttcagg agcccctgac aatatttctg tcaactggtt
    10441 acttgataga aacaccattg ccgcttctct atccctaacc gcctgaaaag acagatccgt
    10501 cgtacttgca gctccaccac ctggttctgg atgggcccct ttgtctacca gatttttacg
    10561 cagctggaaa aaatctgtta gatagtctgg attttcaaat cccaagctgt tagatagatc
    10621 ttcccgatta taaaaattca atcctttctc aacttgtggt atttgaataa tacagccagc
    10681 tataaaatct tcgaatcttg aaatagcata gattcctaat tcttcagtga tttgatagct
    10741 gatttgttca aattcatccc atgtccaatt tgaagctggt tcagggatat ttaattgttc
    10801 aaatatattt ttattgtaag caatcgcaaa cgaattcacc ccgttagaca gtccaactaa
    10861 ttgcccgtcg taagtcgtcg ttgctaaata ggattcatct gcattagaaa tatcaattat
    10921 cccttgctcc acatattcat ctaatggttc aatctgattg atgtattgag gaaattgatt
    10981 gttcagttgg aaaatgtccc agatatcatc tgatgctgct agtgtaccta attttgtgta
    11041 atatccttca taatcataaa attcataatc gatcgtcaca tggggatttt tttcttcata
    11101 aagctcgatt acttttactg ttgcatcgtg tcggacttgc gaaccccacc aagccatgtt
    11161 cagcgtgatt tttttgtcat cttcttgccc acaagccgtc aagaagaata acataacact
    11221 taaagctaag atgattctca ttcttttctt cattctctgc acctctactt ccattgttat
    11281 ttttcaatat actgtatttt tatacagaac atatttatta aacgatcttt ttcaagggga
    11341 ataaaaacca agacttgcct tatcgttctg cctaggatga acaactcaat actttcaaga
    11401 aaaattagat agtgctgatt cttctttcat cgctaaaaac acttcttgct tcaatgatgg
    11461 gaggataatt acgcaaagaa aaaaggatat cagacaactg cccaatatcc ttgacatagc
    11521 taaataagca tgcttcttcg tgtgcgctct tccctaaaaa acagaaaaat aactagtaat
    11581 tctgtatgaa attaggtaca atatacacgt cgatgcacaa gcattgacta tggcaacttg
    11641 attggtcagt tcccgctgac ttttcaagcc ccgacaattt agctgctact aaattgtcgg
    11701 gtgccttttt tagtttttat tcgtttttca ttctatagaa agcgcttcaa taaatcaaga
    11761 catttttttg ttagaatgct aataataaca aaaggtttta actcttctta tgtacattac
    11821 aggtaaaaaa acaaaaaaat ttctcttttt tttgaattct gaacaaatag tactggagta
    11881 caaaagacga ggaagagcca ctatctgctc ttcctcgtct tttgattctc acctgttatt
    11941 catgattctt tttctcagtt tcccgatatt tttccatttt acgactaaaa agctctccgc
    12001 tacttggtgg cgtatatgtg atggcatttg ctcctgcttg aatggtctca gcaatacttt
    12061 cttccgttgg accacctgtc gcaatgatag gaagttcagg atatttttta cgaaaatggc
    12121 gtactgcttc tgcagtttct ttacctgcac tgatattgat aatcgaaata ccagctgcta
    12181 acttttcatc tatttctgta tatttcgacg ttaccgtact gataactgga atatcaacta
    12241 ctcgacaaac ttcttttact gtctctaaag gcgtaggacc gttcaaaacg acccctaacg
    12301 aaccttgcgc ttctgcgaac atgctcatgt aggaagaacg agcaccttgt gtcaaaccac
    12361 cacctacacc ggagaaaact ggaatgtcag ctgcctcaat aatgctttta gtaatcgctg
    12421 gatgaggtgt aaaaggatac acagcaatta ctgcattcgc atttgtatta cgtatgatcg
    12481 caatatccgt agtaaagatg atcgaacgga tcaattttcc aaatatcttg atcccgcttg
    12541 cttctaaaat gatttccggt actcttacga tatcttttct caattccgtc atgatttctg
    12601 gcgtaaattg ttctgccatc agccacttct cctttgctat ctccggtcat actgattcaa
    12661 ttgatacgtc tcgatgacct ggatgatttt ttgcgcataa ccggggtctg tcgcataacc
    12721 tgcatcttgc aatgcttgtg cggcttcctg atagttggtg gccgtaatca ctttttcata
    12781 cagcgcagga ttccaatcta ctccttggac gaacagttgt gtatggtcat ccatggactg
    12841 ttcccatgaa tcatacactt tgaagtctcc ttggatagtt atccattctt cgttaacaaa
    12901 ttcctttgtt tccaggctta ctttcttctg atccccataa gccttgatcc cgaaaagatt
    12961 attgtatttg ctagccaagg tactttttcc ccaatttgat tctaatatag cttgtcctaa
    13021 aatgatacta ggaagcactc cataaccatc ttgtagttct tgggcatgcg gaacaagccg
    13081 ttcaataaat tgctgatggg attgcgcttc attttcttgc tgattatcag taaaaggaga
    13141 agacagattc ttcaaggaaa aaacaaaggc taagagtatc actgctacac cggcaaccac
    13201 tgctggccaa cggatattct tgcgcttttg ctttttccac ttttttttcg ccatgatcaa
    13261 ttcctacttt tcatttaatt cattcagata ttcttctagc gtcaattggt gtttggtgat
    13321 atactcagcg ttttctttac cgacataacg taagtgccaa ggttcatatg tgatcttagt
    13381 tatatcttcg tgtccttttg gataacgaac gataaatcca tactgcgcag cattatctgc
    13441 gatccattta gctccaggct gatctccgta acttgcatcc aatacagtag aaggataact
    13501 gttgtaccag ttttgatcga cgacatcgac tgccagacct gtgtggtgtt cgctgtatcc
    13561 tggctccgta atcgtttctt tagtaatctt tgtcgcctcc tcttctgata caccttgacg
    13621 actcatcaca tcttggacat tttgttcgaa aacttgttgt tgggatgata cagaccggta
    13681 agctgagacc atcactaatg gaaatccagc tgcttcagca gccttggcaa atgcttcata
    13741 ttctggagca atgcgtttgt cgatcaagta tccgttactt aatgatgcca attgagtaga
    13801 ctcatcaatt tctttttcta atttgtgatc tggaccaacc aaaactagct tccaatcgtc
    13861 tgttttaaca tctggcaatt gtttggacgt acgatctttt gtttctttgt cctttacctt
    13921 gctgctggtt gtttgactgt tcttttcttt actagcagtt gttttggtgc ttgttcgttc
    13981 gactgcttga ttgccggctt gaacatgcga atcatcatcg ctaaaaataa catagcccat
    14041 tgctgcgatc ataatcacaa ctgctccaaa tcctaaaaat ttattcactt gaaaaatcct
    14101 ttcgttgttc ttcacgttcc ccattcgcct catgaacgta agtttcttcc agattttcat
    14161 tcacccaatc gagaagttcg attttttctc cttcgatctg tttttgaaca tctgctggca
    14221 gacgaaaatt caaaatatca tcatagcccc attcatcata ttgaatactt actttcaagt
    14281 caaggtatct accagcctgc tgattatttt tcaaagggat cagctcgact tttgctgctt
    14341 tgctgctaag ccatttttgc gcgatgcgtg ttttgatttg cttgtactct gcaacggcaa
    14401 cgtttcgttt ttcgggaaaa ataatcgttt gacgcaaaat cttttgcgtt agcatccatt
    14461 cataatcagt gaaaagattt tttactttct gcatcgcttc agatggcaga tttttctcgc
    14521 catctttcca tttttgccat tgttctggac tgatcagcaa gtattcatct aaaaaatttt
    14581 tttcagtttc aaactgttct aataatacac tcataataag atcaatatat acttgatgca
    14641 cgtttatccc ctccttttaa ctattctact taagttttga tcaaaaagca cctcttaaat
    14701 cgctgctttc atttttttta atgtttcaag tcctctcctc tttattcttc gtgatttttg
    14761 ttataataat agacagacaa gaaactcaat ccaactatga aggagcgttt tcagatgaaa
    14821 aaatatcaat ggggaatcgt tggtcttggt accatcgcac atgagtttgc cgaaagcttc
    14881 aatcaagaga cgagcgaact aacagcagtt gcttccagaa cttcagaaaa agcggaaaac
    14941 ttcgctcacc gttacaatat tccaaaagct tacggaagct atcaagaaat gttagacgat
    15001 gctgaaatcg acatcgtgta cattgctgta ccaaatagac agcatatcga ccatatcttg
    15061 gcagcattgg aagcaggcaa acacgttcta tgtgaaaaag ccatcacgat gaataaaaaa
    15121 gaactggctg acgctatgcg cttagcagaa gaaaagaatt tgatcttagc agaagcgatg
    15181 acaatcttca acatgccact ttatcagcag ttacgctcaa taatggatac aggaaaactg
    15241 ggggctttaa aaatgatcca agcacctttc ggcagttaca aagaaccaga ccctaaaaac
    15301 cggttcttca atccagaatt agctggagga gctctgttgg atatcggtac gtatgctgta
    15361 tctttcgctc gtttcttttt gagctctcaa cctgaagtag ttgcctctac aatggttcct
    15421 tttgaaacag gcgtggacga acaatccgtc actatcttgc gtaacaaaga aaatgaactt
    15481 gctgctgtca gcttgacttt tcaggcaaaa atgccaaaag tcggtatcgt tgcctttgag
    15541 aacggttatg tgacgattgc tgattacccg cgtgctgatc gtgcggagat cttatttaca
    15601 gatggtacaa aggaattcat cgaaagcggg aatacatctg aagcaatgaa ttacgaaata
    15661 aagaatatgg tacacatgat tgaaggtgac ttgcccaatc gctctctctt cttgacaaaa
    15721 gatgtcatcg aaatcttgga tcaaatgcaa gttctatgga aaaatatgta attttaaaag
    15781 gttgtgaaaa agctgttctg catcaagtaa taagaacagc caaacaaaaa tagctcctcc
    15841 tatttttcgt ttggctggac ttattaccgc agaggaaagc ttttgaacac cgtttaagaa
    15901 agaggttgtg agcctaccgt tcagctccga gtcacaagga gcaatttcaa aaaacagctt
    15961 ctcatgtttg ttgaatattg tgacttgtga ccgaggagtt gacaggagaa caccgtttaa
    16021 agaagaggtt gtgaaatcag ctttttgacc tgaatattag ctgaattcac aacctctatt
    16081 tttggatttt ttgttgggag aattctgcct tgatttcaat aaaactttta aaagactgca
    16141 aaaactggaa caattctgct cgatcctcga attcagggcg actagtcggc agaggcattt
    16201 gccgatacaa tacataaact tcttcaatac gtgttaaaat ttgtctgcca tcatttgact
    16261 ctgaaaatgt ttctgcagta taaatcaata aaccatagat atgttttcca taataagctg
    16321 gttgctggat tctctctaga ttttccagca tgtctttaat cacattggcc tgtgctcttc
    16381 tcatagagaa gtaagtttcg tagtatagcg gctgtctgag ccagtagttt tcttgatgtt
    16441 ctctggcatc ttcttggctt tcgcgaatga atgtcagtaa atgttcgcat ttgatctgca
    16501 catcttcttt attttctgat aaaagaaatt ctgccatttg tctcaaaata tttcgaaatt
    16561 ctttttcgat ctgtatttgg ttttccttca gctgcttcgt acgatctggc atgtaaacat
    16621 ttgcaagcaa agcaaatact aatccaatcg ccattaatcc cgcttcattg ccgatcagct
    16681 gccaagacat gttttcctcc actaaataat gagtgaccaa aaccgaattt actactatcc
    16741 cttcggttaa tttgaagcga gaggcaagcg ggatgaacag gaataaaaaa agactgaagc
    16801 caaaaatagt atacccaaca gcaaaaaaac tgaacagtgc tgttgccgta gctaatataa
    16861 aggctgcgat tcgattcatg cccgtacgca atgtagagcg cttggtattt cctacactta
    16921 ataatgcaat gatccctgca gctggccaat acaacagcga taagctaccc gctgtgacca
    16981 tcgaaaagaa gacactgatc actgttttga ctgttcgcat tcctatacgc atttacttcc
    17041 ccctttttta ttttaattat acttgaataa tgaaaaacta gctatcagac aaaagtttag
    17101 gtatgatagt aaaaagacca aaaaggagaa tctctatgca atttggccgt tttcgactag
    17161 gtatgcgtac gatcaaaagt gccttagctg tttttttatg tatcttgttt tttcatttga
    17221 ctcaacgagg aatcccgatg attgctgcat tatctgctgt tttttctttg cgtcaggact
    17281 tgactacaac cgtttctttt ggaaagtcta ggatcatcgg caatacatta ggtggtacat
    17341 tagctatcat ttattttttt gtcaaaagct attttcacaa tgattttttg gtagaattga
    17401 tcttgcttcc tattctagtt attattgtga tcgtcgtgtc agatggtatt aacaataatt
    17461 ccggtatcat ttccgcaata gctacgttgt tgctgattgc gttgagcatc cctcaaggcg
    17521 aatccgcttt gtatgctatc caacgtgtac tagatacttt tatagggaca ttcattgcaa
    17581 tcggtatcaa tttctttctt cgtcctcctg aaacagaaaa gaaggaagaa ctatcagaag
    17641 atttggtcga actccaaaag aaagaacgat tgctaagaac aaatttacaa gagatagaag
    17701 ctaagatcca aaaacaaaag aaaaatagcg aggactaatt ttcttttgca ataaaataaa
    17761 aaaatggaga gaagaacttt aaaaagtctt cctccatttt tgattgaacg gtgaagtgta
    17821 ctactttatt cttccttcat cgagttttgt tctgcctctt ttggcgcttc atacatattt
    17881 acttggctag tagtcccacg tgtcgacaaa ttctctacga tttccttcaa gtcaagtcct
    17941 gttgtcgctt ttagtgtttc ttgagtagaa gaaagcaaat ttgtggcata atttgtcact
    18001 cggttcgctc cactggtttc tcctgaactg cttcctgtat cgacaacaga gattttttcg
    18061 atatttccta gcggctgtgc tgcttcgcgc atcaattgcg gtagcatatc gataaccata
    18121 ctcaagacag ctgcttcgcc atattccttg aaggcattgg cgatcttttg ttttgcttcg
    18181 gcttctgctt ttccttttgc tagtgctgct tcggcttctg cttgtccagc taaacgtgtc
    18241 ttattggctt ccgcttctgc cagtgcttcc acacggaatc gttctgcttc ggcttcagtc
    18301 acttctttga ctttttgagc ttgtgcttct tgttctttag cgtaccgatc ggcatctgct
    18361 tttttcttca cttccgaatc atattgcttt tcacgacgtg tgatttcttt ttcttccagt
    18421 tcgatttgtt tttgacgttc tacgatttta atctgcattt cctgttcgat tacttgttgt
    18481 tgtgctcggg cactttctaa attgtaggct tggtctgctt ttgctttagc aacatcttgt
    18541 tcttgtttgt aggttgctaa tttcagttct ttttctttta atgattctgc gatctctgtt
    18601 tgtcgctgta attcagctgc ttgtgattct ttttctgctt gagctttttt gatacgcgtt
    18661 tcttttagag cttccgcttc tgctatatct gcatctcttt tgacttgggc gattcttggt
    18721 ttccctaaag agtcaagata accgtttttg tctctgacct ctttgatagt gaaagaaacg
    18781 atgatcaacc ccatttttgc aaggtctaca gaagcgactt cttgtacgct ttgactgaac
    18841 ttatcgcgat tttgatagat ttcttctaca gtcatagaac caagaatcga gcgcaagtgt
    18901 ccttctaata cttctcttgc ttcattttcc agttcttctc ttgtcttgcc taaaaactgt
    18961 tctgctgctg tagcaatctc ttctacagat gaaccgattt tgatgatcga agtaccatca
    19021 cacatcaccg gtacgccttg ttccgtatac acttcagggg tggagacgtc caatttactc
    19081 gataatagac tgatccgatt cgaacgttga aaaactggca agacgaatgc gccacctcca
    19141 cgcacaattt tgattttgtt attgctttca tcaatgtgca catttttgct tcctaaataa
    19201 cttccactga tgatcaacgc ttcatccggt tttgctgtct ggtacttcgc tacaaagacg
    19261 atcagcaaca tcagtaaaat caaagctgcg attgcgatcg cgataaaaac aactggtatt
    19321 tccatttaac acacgctcct ttaagctttt atcatgcttt cataaggcat gacataacag
    19381 attctttcct tgatctcaat gatcaatact ttcgttcccc gctcagctgt ctgttcttgg
    19441 gggctgtaaa aacaagctgg acgactaatg gaaccggtaa cacttttcaa ctgaatttct
    19501 cccatacctc gtacagggat cggtgtgatc acaacagcta cttgtccttc cagagttttt
    19561 tcagaagacg acatcgttga ttcggcatgt ttcatcggca tcaatatata gaaattaagc
    19621 aaaaatacca acactatcga tatgcctcca ctagcaataa ataaaaataa agatgattga
    19681 tctgttaaac gctcacctag aaaaccaaat aagcttgtga aagttaacca agggataata
    19741 agcatcggat caagcgggcc atcaaaatcg aatacatctc caaaaataat caataggact
    19801 gccaatcctg cacaaatcag caatgtatac caataaatcg tctcaagtgc gattccccct
    19861 atcatataca cgcctccttc catttctacg tctatcatac caatgtttga aaattctgta
    19921 tagcttcttt taccgactca gtggtgtttt ttataggaag gttgtgttta tcgttgacaa
    19981 tttgttaaat ttttgttttc atgttaaaat tttcacaata aattctttcg aggtagccta
    20041 tgtttccttt ttttaagaaa aaaccattaa agctccagaa cacgatcatc cttgtcgttt
    20101 gtttctccac attcgtttct ttgattgttt ctgccatatt gatcaggaat tttattcttt
    20161 cgatgaaccg atctagtgtg tcagataaaa tctctagcat tgctaaaatc gttgccaatg
    20221 ataaactcat ccaaaaaaag ctgcagactg gtgacccgac acatgcgatt caaggaataa
    20281 caaatgaaat catgattgca tcgaatttag attttatcgt tgtcatggac cctgaaggca
    20341 ttcgatattc tcacgcagac ccaagttaca tcggtcttcc tttctcaagt catgaagatg
    20401 tgaaaaaagt gattggagga caagaatttt tctctgaaaa agaaggaatg ttagggttcg
    20461 gcttccgcta tttcgtccct atttattatg atcaagagat tattggtgca gtttgtgttg
    20521 gcttaacaaa caacacgatt gacaatcaag tcaaagatgg acaaaaaatg attgtttttt
    20581 cattgattat tggacttagc gtaggcatca taggcgccgt ttttcttgcc caaaaaatga
    20641 aaaaagtact gcttggactt gaaccacaag aaatagcaga acagttaagt gaaaaaacga
    20701 ttatcgaaaa tgaagtagat gaaggaatct tagccatctc ttctgataaa actatcctct
    20761 tgatcaatac acctgcaaaa aacattttct accaaacaga gcagctattg gatttcgaag
    20821 taggaaaaaa atcagtgata atctatatga cgttttgttt aaagagacga ttgagcatca
    20881 aacgaccgtt cggactcaat ccatgtattt aaatgggata gaaactattt ctagcgttac
    20941 acctcttttt agcaatgacc gttttttagg agcagtcgca actttacgaa atcgatcaga
    21001 agtaatgaaa ttaatggagg agcttggggg gacgaaacaa tatataaact ccatgagagc
    21061 tcaaacccat gaattcatga ataaacttca tgtcatttct ggtttgattg atttgaagaa
    21121 gtatgaagaa gtcacccatt ttatccagca gctaaatcaa aattataaaa aagagattgg
    21181 ccaagtctca agtctgatca aagtacctgc aattgccggt tttttattag gcaaaataaa
    21241 cgaagccaaa gaacaatctg ttgaaatgct gttagaaagc gattctttta tccctaaaat
    21301 ggagatggat gactccatcc attcactttt gcaagtgtta gggaacttac ttgataatgc
    21361 aaaagaagcc gtgctacaaa aacatgaaag aaacggacag gtccggattc atttaaacta
    21421 tgacacagaa gctgaagtat tcatcgtgac cgtctgcgat gacggtgtcg ggatatcaga
    21481 agaaatctca gaaaaaatat ttacgctagg tttttcgacg aagggaatca atcgtgggtt
    21541 tggattgaat ctcatccatt caatcgtcaa acgccacggt ggatttttag aattttccaa
    21601 acaaacaaat ggcggtacaa tcgtctatct agaattacca aaaaaggaag atcaaaatga
    21661 tgacaaatgt cctgataatc gaagatgatc caatggttgc agaattgaat cgtacttttg
    21721 tggaaagcat ccctaatatg aacgtaatcg aaagtttcag aaatgtagaa gaagggaaaa
    21781 aatatttcag acaaaaaaac gcagatctgc tgctgcttga tgtatacatg cctggcgaaa
    21841 gtggtatcca ttttttatct tggcttagac agcaaggaaa tgatatccca gtgattttga
    21901 tcactgctgc agatgatatc gaaaccataa agaatgtgat ccgctacggt gccttagatt
    21961 atttgatcaa accgttcact ttgcaacgtt ttcagctggc gatcgaaaag tatcttgctt
    22021 tctctcagac aatccaaacg actgaaaaag ccgatcagaa attgatcgat caagtattca
    22081 accacaccac tcccattcac gaaataccat cattcatcga tttgcctaaa ggtctgtcaa
    22141 aactgacttt aaaaaaaata agtgcattta ttctaaaaga agatactatt ttttcaacaa
    22201 aagaactggc tcaaaaaagc tcactgtcac gtatatccgt caagaaatat ttgaattttt
    22261 tagtagaaca agatatattg aacgaaagaa tcaaatattt ggaaatcggc cggccgctaa
    22321 cctgttatga attgacaaag aatgggcgga aaaagctcac agattactgt tgagtcatcg
    22381 aattatccca tacaaaatag tccaagtata cagaagtaac aactgtttac ttggactatt
    22441 tttaaacatt ttattgacga atatccatgt tttcttatct taattgacgg gctttaggaa
    22501 gagatacctc atttttcgtt tttccagttt ctattatttc acgaaagttc tcgaaacttg
    22561 tcgaaatcat atttgataat gcctcatctg tatttgaacc tacatgcggg gttacaagaa
    22621 ctctagggta catttctact aattgctgga tagcagggtc tggaatcgtc tcccatggct
    22681 tgaaggatcg gaaaaaaacc tctgtttcat ttgcaaaaac atcggttgca aaaccagcta
    22741 aatgattgct tttcaaagct cttaaaatag cttcattatc ttgaagctct ccacgggctg
    22801 tattaatcaa gatgctacct tttttcattt gagctaaaaa tccttcattg atcatttgat
    22861 cgttttcacc tgggatatga ggtacatgaa tactgacaat atcgcttttg gctaataact
    22921 cttcaagcga gtggaacgtc aatacttttt tagcagcttc tgtctgatga atatcgtagc
    22981 cgatcacttt ggctcctagt cccttaaaca aaacagcttc cgtcaaccct atcctgcctg
    23041 ttcctatgat ccctactgtg caatttctga tttcacgact aaacatttgc tcatccactc
    23101 gaaaatcttt gaaagatgtt cggatcgtcg tatacgcagt atttctcaaa agcatcatgg
    23161 caaaagtcaa agataactct gctatcgcat ttggcgaata cgatggtaca cgggctactg
    23221 tcataccaaa atcagctgcc gcttgtagat cgatatgatt gactcccaca gtccgtgtaa
    23281 aaacataacg aatcccatat tcctgcattt ttgcaagatt ggtctcatct gctatacaat
    23341 ttcccctcaa caatacagca tcatggtcct ttgctgacac gatattgtca tgtgtcaaca
    23401 gttcttcata aagactgaga tcataatggt aaatattcaa tttttcaaaa aaatctatct
    23461 cgttcggacg aacaccgtaa catgcgattc tcatactttc ttcctccatt tcttatgaaa
    23521 ataaacctat gccatgaatc atttgtaata caacgatcca tacaggtgtc agtaccacag
    23581 caaaaatagt tgacagtaag gatgcattgg aggctaaaag tgcctcccta tcaaaactga
    23641 tagcataggc tgctgccact gttgcagctg gtgtagccat catgataacg attgttgcca
    23701 gtgcttcaaa attgactggt agaatattcg ttgccgtcaa aactgctaga atcacgatat
    23761 tcaatgcagg tacaaatacc actttgacta gactgtaata ccatgaagtt ttttcagatg
    23821 cagctttttt gaagcttact tcacctaatg ttgcgccgat tgctagccac gccaatggtg
    23881 aacttaaacc agctaaataa tccatcggct gaaatagcca cggtgcagtc tggtcaattc
    23941 gcaaaaacgc tacttggctt atctctcctt ctgcacctgt aactgacact tgtggcagat
    24001 acccttggaa cagccaaatg aaaaggccgg caaaagtagc gataatgatt ggattgacga
    24061 acatcgcttt gatattttta gcttccatct tcaagccact catttttatg tatccataag
    24121 aatacaaaaa gattcggtag ccaatattga aaattgagga atacatcacg ccgacaggac
    24181 cataaatcgc actgacgata ggcgttccga aaaaggtcgt agaaccaaag atcgtcagga
    24241 cacgcaacgt tgtttgctta tcccctttat agcttaagaa aaaaggtttt gaaactagaa
    24301 taagaataat ataaataact aatccccaaa tcaagacatt gattccttgc atcagtaatt
    24361 ctgtattgat atcttgcatg aaagctcgaa atgccagtgc tggcaaagcc actgttaaga
    24421 cgactttcga tagaattttt ccaacagatt cagaaaaaat cccctttttt ctacaaacaa
    24481 accctaataa tataataaaa actgttgaag tgattgcact gacaatcgac atatctgtca
    24541 aagtcgatcg aaaaatgtca aataagttca tctttctcct cctaattatg ttcatttatt
    24601 cacaataaaa aaagctttta ttttgctaat acgtattaac atttttaatt ttatctttac
    24661 attgttactt attgaacaat aggaaattaa agaaactttt gaaacttatg taaagtgatt
    24721 tttactagaa cac
//
LOCUS       DAZKLD010000027        24410 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.027, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000027 DAZKLD010000000
VERSION     DAZKLD010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24410)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24410)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24410
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.027"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..522)
                     /locus_tag="ACG8KR_000910"
     CDS             complement(<1..522)
                     /locus_tag="ACG8KR_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0200305.1"
                     /translation="MKINKKVVILSGLIIVSSIYSFIFFKNTQSIYTTGDLSFHLSRI
                     KGLSTIFTSPINYETFNYTGYGVNYFYPFLTFFPAVMLYWMTKNLIVSYIIYVWLLNL
                     CTTLVAYHYGERFLKQKKAAFLFSCLYIFSAYRTVDIYYRSAIAEAIAITLVIPVLFY
                     AYQIISGKEEKYPS"
     gene            complement(509..2197)
                     /locus_tag="ACG8KR_000911"
     CDS             complement(509..2197)
                     /locus_tag="ACG8KR_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0200306.1"
                     /translation="MKISKKAFLLVVLILLSTLYSVNFMRNAQEIYTTGDLSFHLSRI
                     KGLSSIFEGPINYTTFNNYGDGLNYFYPFLTIIPAVVFYGISNNLILSYVLYIWLLNI
                     CTILISFYYGEKFFKKIDAAFLFSCLYTFATYRTVDIYYRSAIAEAIALTIIPAVLYY
                     AYGLIFNREKKVIALSVSISLLVYTHVLTTLLCSALIACMIIVRLFLSKQIKNDIKFV
                     VVNLVKAVFLTCALTSYFWYPMLQQMNYQEIHSPFKTDLQLEALSISDSIIQALNNDI
                     TTYTMGLLGLVALIFPLFILKEMEKKEKIIYVGVVSTWVCVTNLFPWFLFQKTPISLL
                     QFPWRILGIQIFFSSMIITLVFMKKTMNKKYSWLIIGATVVFLLVTSIAAKENYAKVI
                     QSKHGHIVINEETLPRYTTSDMGGLYDYAPTEAIKERPHLEKHEVKVGESWEKGKYKA
                     ADNNITYTVASNKKQKVTVPVYAYLGTQVKVNGKVVNDSYKKHGLVNVDVNAGENKIE
                     ITTKYTPTALMALWISILTYCLVVYSSFKRFAPNKLKDSRYVTKKEINKKNENK"
     gene            2343..3980
                     /locus_tag="ACG8KR_000912"
     CDS             2343..3980
                     /locus_tag="ACG8KR_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0200307.1"
                     /translation="MKKNKNQIIDIIFMLFLSFAYVIPLFMSKGIYHSVNQDTYFHLS
                     RIIGLDNVWSSPVNFNNFDHHGTMMNIFYPWLTLYPAFLFYKMMGNLVLSYNIYYFFI
                     TFLTMVVSYFSMKQIKNNRYISLLFSIIYTFSAYRAIDIFHRASLGEAVALTFLPLIL
                     MGCYEIYIRDYQKWYWLSIGMTLVVYTHLLSVAMVSVFIGGTLFLSFYFWDQKIARLL
                     SLLKATVLTFFLSAGFLIPFIQQSRAQELKVPLGKELSGMAPSDMLTHILNNNYNNYT
                     IGLFLFLGLIGAFIFIKKLTADDLFIFFLGVFVLFCSTNLFPWQLFNHTPVKSLQFVW
                     RLNGFSSLFIAYTMSIVIYYIFSTKNNSWKIMVFTFLALILHLSGVSNSIHANKDSLT
                     LIAPEDAVAIAENYNHTDYANKESIYHPDMINNDQYLLGNQEINPTTHFMSNKLTIEL
                     DNSNNKNTVLTTPIYRYKGQVASINGKLVQTKLSKFGTTELTIPPGINKVVITYQYTK
                     LAIASRYLSIVTLILFLLYRFTFSKQKQPRREVQHSH"
     gene            complement(4125..4865)
                     /gene="truA"
                     /locus_tag="ACG8KR_000913"
     CDS             complement(4125..4865)
                     /gene="truA"
                     /locus_tag="ACG8KR_000913"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HFQ0200308.1"
                     /translation="MRNIKLTIEYDGKRYSGWQRLGDDDKTIQGKIEKVLHQMTNEEI
                     EIIGSGRTDAGTHARGQVANFKTNTEIGLAEMIDFMNRYLPRDIVIKRIEEMPERFHS
                     RYNAVGKKYSYYVWNNVIPSAFERNHSFYFPQELDMDKLNAACEKLVGTHDFIGFSAL
                     KKSKKSTVRTIDELSIERKGEMLHFTFVGDGFLYKMVRIIMGTLLEIGTGAMPIETID
                     EIFESKVRQHAGETVPAQGLFLDEVYYR"
     gene            complement(5056..5691)
                     /locus_tag="ACG8KR_000914"
     CDS             complement(5056..5691)
                     /locus_tag="ACG8KR_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HFQ0200309.1"
                     /translation="MDFLRKFGFFFLLLGVLSDFLTPYVLGLYYPELDQMKSVISVFG
                     EVSSPVRKAFLIWSVVSGIFFILSLPALYAAFVSTSRVLATLVTAAIAFYGIGDCIFT
                     GLFSINTHESSWNVSTWVHNIGSGLGYSGFLLFPVLLFLIYRKNGQMDVSHLYLGLSV
                     ISLLFAAIYGLARIPSINSWPLLNKIGFCQRISFFFNYVPIAWLSILHLKQ"
     gene            complement(5838..7058)
                     /gene="sstT"
                     /locus_tag="ACG8KR_000915"
     CDS             complement(5838..7058)
                     /gene="sstT"
                     /locus_tag="ACG8KR_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0015826 - threonine transport [Evidence
                     IEA];
                     GO_process: GO:0032329 - serine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HFQ0200310.1"
                     /translation="MSLVQRIVVGILVGILFGIFFPTWTFISILGTLFVSCLKAIAPI
                     LVFFLTMASIAKHKIGNKTFVKPILILYLVGTLLSALVAVIVSSIFTVPITLQETVSE
                     KAPQSLESVLSTMLTNVTQNPVQSLIDANYLGVLFWAVLLGLAFRARSESTKELVDQI
                     SIALSQVVQVIIAFAPIGILGLVYQSIATTGIAGLAEYLQLLLVLIGTMLFVALVVYP
                     FLTFLFIKENPYPLIFFCLKESALPAFFTRSSAANIPINMMLAERLKLTKESYSISIP
                     LGATINMGGAAITISLMTLTAVHSLGIEAPFVLKVLLCILSALAACGASGVAGGSLLL
                     IPLACSLFGISNDVAMQIVGIGFIIGVIQDSMETALNSSSDLLFTAIGELGARKRSGE
                     KIKLKDCLDGLSER"
     gene            7277..8275
                     /locus_tag="ACG8KR_000916"
     CDS             7277..8275
                     /locus_tag="ACG8KR_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0200311.1"
                     /translation="MFLSNFFVRCLFLKKIKKESYGFLTIFVACIMMVLQRILISAVT
                     IPTYATNPGRFYIYTFLQTSVVIGANLIPLYMGYQYQKIARLKVLHYLSRFAFIFLIA
                     TLVCNIFFYVQAGSLNIRDYWLILTPISQNYFTYAVSCTLLFCSIPYTVGFLNKLSKT
                     TIKYLLLFLTIGLVGCSTLFNKDPFALQNGQSILWIFYLFLMGYGLKEWNWKEKVRYK
                     LPQVFISLIVLFGLIILMTQISLIIRGNTDTALRFSKPYSLFAMYYTFSSFLFLEMLS
                     DKLGIKGRGNFLAVFLIVTQVICNFPLVSYRVSTFMKREFPNSGKAWLINIGQFFI"
     gene            8471..10306
                     /locus_tag="ACG8KR_000917"
     CDS             8471..10306
                     /locus_tag="ACG8KR_000917"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFQ0200312.1"
                     /translation="MQIFVLEPKEGWKETIQVALKIFTQRQAPMILTIIIIMGFFFLL
                     LLISNRFWYALTATLIINLLLTISTVIKMEMREEPVFPSDLKMLTGLSELLSMVSPVL
                     LIVGGLILLLLLITSIIVQRRLQKQYSLKIHWKRRFISIAVLLGCFSGVFFINHKNSP
                     SFLLFNLFKVNKTFFNQQGAVKDNGPVIQFLNNIDIKIMEEPAGYSKEKINGIMKKYN
                     TKADEINQTRSDWLNNQTIVLNLSESFSDPARVPNLTVPTDPIPYIKSIITEGTGGLM
                     LSVGYGGGTANMEWEGLTGLSISNLSASLVTPYTQLVERQKISPNITDLFDEKIAIHP
                     FTAALYRRKQVFDKFGFDRFYYLDSPDKLTYTDKIEQSPRISDDSAYKETLKALQSNT
                     ETSQFIQLSTMQNHMPYDDYYSELNYTAEGTAVIPNRRKELQTYMQGLHYTDTAVKKF
                     IQEIDKIDKPITFVFYGDHLPAIYSGNDSKKYGLEQHQTDYFIYNNKYSREQAATLNK
                     EVVAPNNFGAMALEHANIKVTPYYALLTEVSNHLPALTIDPTESLSNRFNGKQVFVTE
                     DSQIIQEKNLTEKQKEILEDYRLIQYDLVAGNQYSATWATQKIKD"
     gene            complement(10366..11082)
                     /locus_tag="ACG8KR_000918"
                     /pseudo
     CDS             complement(10366..11082)
                     /locus_tag="ACG8KR_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299292.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
     gene            complement(11309..11851)
                     /locus_tag="ACG8KR_000919"
     CDS             complement(11309..11851)
                     /locus_tag="ACG8KR_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HFQ0200313.1"
                     /translation="MNIVEEKFVQETKQADIDHHKPTAGAMVGHVLANFKLEEMKLIQ
                     AQLYVLGTSSQELSTLFSEYADQEARWFNKISHELVIENEIVPSTLEEVMRYGKIYEH
                     GQNKYLPASTMLENFAKDFDFQNVFITRAIKLAEKEEKYGLQQLLVELLSFNKEIIYQ
                     VQRLQGKTVRQDLDEEDDDD"
     gene            complement(11856..12083)
                     /locus_tag="ACG8KR_000920"
     CDS             complement(11856..12083)
                     /locus_tag="ACG8KR_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HFQ0200314.1"
                     /translation="MKKIIMQLDTLACPSCMQKIEHAVSQQPGVSNTKVLFNASKIKA
                     NFDPDEVHPEQLAAVVEKLGYEVQSAKVKEA"
     gene            complement(12162..14042)
                     /locus_tag="ACG8KR_000921"
     CDS             complement(12162..14042)
                     /locus_tag="ACG8KR_000921"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFQ0200315.1"
                     /translation="MKLQKWIQEHRNHITALSAVLIIFGFIGKFTGFVLFYNIALAIA
                     SIIAVIPIMIHAYQAVKVKVISIDLLVTIAVLGAFMIGEYKESAIVTFLFLFGSYLEQ
                     KTLTKTRESIKALTEMAPSTASVVQEDGTTEEVEIDDVEEGDIVLVKTGGAIPVDGRV
                     IDGKGYADEAAVTGESRQVVKISGDEVFSGTILADGYVTIEATKVGDDTTFAKIIELV
                     EEAQDTKSHAEKFIDRFSQYYTPTVLLIALVVGLISRDFRLAITVLVLGCPGALVIGA
                     PVSNVAGIGNGAKRGILVKGGEAMDTFSKIDTFVFDKTGTLTKGTTAVSTIKNYTEDE
                     GDSLALVARLETLSDHPLGRAVIHYAQEKDIDFQQLAVKDNQTIKGQGITAEIDGHTV
                     CAGNTKLIAAHKLSLTPKQVQDLHQLQQTGSSVIIVAIDKKITQIIGVSDVIRPEVAE
                     QLAKLKQAGAKQLVMLTGDNQMTADYVADMLGIDEVHAELLPDEKVQFVKKYQEQGLR
                     VAFIGDGINDSPSLAAADIGIAMGSGTDVAIETSDVVLMQSNFESLVHAYRLAKKTVL
                     NTRENVVIAIGVVLFLLNGLFAGFIYMASGMFVHEASILIVIFNAMRLLYFGHESKEE
                     KS"
     gene            complement(14231..14845)
                     /locus_tag="ACG8KR_000922"
     CDS             complement(14231..14845)
                     /locus_tag="ACG8KR_000922"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0200316.1"
                     /translation="MKLTFKPLEKKDYKKAIYFAVEGMNFERYMENELLLNLYGRYFL
                     YMELNRATQILALYAEDELAGVLLAEIKGEKKKHRSFGQQIYVKIFDFLQNIFAKESK
                     GAYETANEQMLAAYLKKHSPDGEIIFLAANPDLKTKGIGTKLLTEFEKREKGKEIFLF
                     TDSGCTYQFYERRGFERLKEKISKIKILDKEIDLRCMLYRKIIQ"
     gene            14987..15547
                     /locus_tag="ACG8KR_000923"
     CDS             14987..15547
                     /locus_tag="ACG8KR_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFQ0200317.1"
                     /translation="MKKTDRRVKKTEKALAETLSTLLVNKKIQAITIRELTETADVHR
                     STFYTHYKDIYDLYDQLESNFFRDLNEILSYDPAHSYEELYTRLIDYVYANTFLYRLF
                     ILNHGDISFQKKVKQVIEENYLNIWLYEDKKTIITEEMRFFTTYHVQGCLSVLNRWVE
                     SNYKYSKKELLELLQKLNDHIEKIMP"
     gene            complement(15852..16166)
                     /locus_tag="ACG8KR_000924"
                     /pseudo
     CDS             complement(15852..16166)
                     /locus_tag="ACG8KR_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
     gene            complement(16420..18055)
                     /locus_tag="ACG8KR_000925"
                     /pseudo
     CDS             complement(16420..18055)
                     /locus_tag="ACG8KR_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294751.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(18494..19363)
                     /gene="scrK"
                     /locus_tag="ACG8KR_000926"
     CDS             complement(18494..19363)
                     /gene="scrK"
                     /locus_tag="ACG8KR_000926"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008865 - fructokinase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase ScrK"
                     /protein_id="HFQ0200318.1"
                     /translation="MYGSIEAGGTKFVCAIGNDDLKVLERVSFPTTTPNETMSLVIDF
                     FNHYKEQLESIGVGSFGPIDIHRESKTYGHITSTPKTAWKNFDFVGTLNKHFEIPIAW
                     TTDVNAACYGEYVSGHGKGLSSVVYYTVGTGIGGGAIQDGIFVEGFSHPEMGHTLVKR
                     HSGDTFSGTCPFHHDCLEGLASGPAIEMRTGTKGQDLSIEDPFWEIEADYIAQCACNT
                     TLMLSPDIIIFGGGVMQQEHLKKKVQRRFLELINGYVDTPNIEEYIVTPKLADNAGTI
                     GCLTLAKDVRINS"
     gene            complement(19383..20504)
                     /locus_tag="ACG8KR_000927"
     CDS             complement(19383..20504)
                     /locus_tag="ACG8KR_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 172 protein"
                     /protein_id="HFQ0200319.1"
                     /translation="MNCLNNIWLHKGEESRTISPENKNGEKGKAAMTASHLGIGRKGS
                     PSVPINAGETITLAEMEGPGILRHFWMTVTDKTIAGSFVLRDLILRIYWDDESTPSVE
                     SPLGDFFCNGFGTRCEVNSLPIVVNPTSGMNCYFEMPFRKKAKITLTNEHPEFIKHIF
                     YTFNYALTEVPDDAMYFHAKWNREPITTKGKDYTLIDGIKGTGYYVGTYFALCALERY
                     WWGEGEFKFYIDGDEDFPTITSTGAEDYFGGAWAFHKKNFGERAYAQNFNTLFMGYPF
                     QSKRDHTRDYFSAGKPNPNPVHGFGDDALPMHGLYRWHLPDPISFKENLRVTFQTLGN
                     DDIKLYERQDDISSVAYWYQKEPHEFFEKLLDREERIPR"
     gene            complement(20506..20901)
                     /locus_tag="ACG8KR_000928"
     CDS             complement(20506..20901)
                     /locus_tag="ACG8KR_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFQ0200320.1"
                     /translation="MRRLIIASHSILAEGFKETLEFLTGMHNQISSLCAYVEEIDDID
                     EQIKELVDTTDEIIVLTDMAGGSVNQKFFTYISDRVHVIAGMNLPLAMSIALQLGNDN
                     LDIGAIIAEAQQQIIYMNNQKSEERIEDE"
     gene            complement(20920..21717)
                     /locus_tag="ACG8KR_000929"
     CDS             complement(20920..21717)
                     /locus_tag="ACG8KR_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFQ0200321.1"
                     /translation="MSEKYQVTKKDLRKANYRWLMSVCTFNYQTQQGASVTYALSPIL
                     RKLYKDDDEYIEALNNHFQYYNTQPWLAAIILGACVAMEEKQGLEAKDAINDFKVGTM
                     GPIAGIGDSLLMTMIPTIMGSIAAYMALENNPVGIFLWFILIMVIFFFRMRGFEFGYK
                     QGMKIVTEYGEKINYLTEAASVLGLTVVGSLIASVISVSTPLKFTFGEVSMKIQPMLD
                     KILPTLIPVIITAAAYYLLSKKKISMTILILLVIVFAMVMAAFGILA"
     gene            complement(21719..22507)
                     /locus_tag="ACG8KR_000930"
     CDS             complement(21719..22507)
                     /locus_tag="ACG8KR_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFQ0200322.1"
                     /translation="MHFTIFQIIMLTILAFIKHVDYYGIPMIFVNYAVFWGLFTGILM
                     GDIKTGLIVGGTVQLMSLGVAGFGGSSVPDYGTCAIIATAFGSQLGVEAGLAIGLPVG
                     MLGVQLDVIVKILNGFVVRKSQEYANKREFKKMNSVLWLGPIFFGLSAAIPVFIAVTL
                     GQSAVNYVLDVLPNWFMDGLTLAGKMLPAVGIAMLLRFMPTGRYISYLLVGFFLAAFL
                     KVPIIGAAIIGFALAFNVYHRAEKDAQITASAGASDVGFIGEDE"
     gene            complement(22521..22997)
                     /locus_tag="ACG8KR_000931"
     CDS             complement(22521..22997)
                     /locus_tag="ACG8KR_000931"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFQ0200323.1"
                     /translation="MSIVMSRVDNRLLHGIIVTQWAPMSKANRVMVIDDSVANNDIQK
                     SSMRLARPAGMAVSIITEETAIANFKKGKYDQEKVFVIVKEPETILHLVDNGIRIDRL
                     IVGGTVVKDNALQLSSRAYASDENISTYKKLLDSGVEIVTQFVPADKAQEIQSKLG"
     gene            complement(23069..>24410)
                     /locus_tag="ACG8KR_000932"
     CDS             complement(23069..>24410)
                     /locus_tag="ACG8KR_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFQ0200324.1"
                     /translation="YVKEIERDFQIEFDYYYLQITSIFLNSWIYQIQLTSDDKLYSFI
                     NDINSWCSREVQLVNRFIQIGDIYLTKIQKNVLVILFKLMCSKEFQQKRLALILAHGD
                     LTATCIASTANRIVERYIYDAIDMPLMTSSSEIADKINRYLQECTGYEEIILLVDMGS
                     LEKIYLNLELDAQKKFGLLNNVSTRMAIDVANQILLDLPLKQILEQVKENQNVDYIYE
                     SSNIKKDMIVCSCASGLGTSFKLKRILEDSFPRDTDLVIETCDYYSLVNENYLSELRK
                     ENNILFIVGTLNPHLEDVEFFSIEDMILGMNISKFSNQLNRHFNDNVLKVLNNNILKN
                     FSLTNLMEQLTILNPTKLLERVSDAIYILQNDLGISFDNNTCFGLYVHISCLIERLVK
                     QNTLEDEIYFNETSEEFQKFQTHFKQSFSVVEHYYSVDIPIHEVKYVYDYVKRA"
BASE COUNT         7810 a         4719 c         3714 g         8167 t
ORIGIN      
        1 agacggatac ttttcttctt ttccagagat gatttgataa gcataaaata atacagggat
       61 aacaagtgtt attgcgatag cttcggcaat tgcggaacga tagtagatgt ctactgtccg
      121 ataagcagag aaaatataga gacaactaaa taaaaatgcc gctttttttt gttttaaaaa
      181 ccgctcgcca taatgataag ctacgagtgt tgtacacaag ttcaataacc aaacgtaaat
      241 aatatagctt acaatcaaat tttttgtcat ccaatataac attacagctg gaaaaaaagt
      301 cagaaaggga taaaaataat ttacaccata tccagtataa ttgaatgtct cataattaat
      361 cggactggta aaaatagtcg ataacccctt gattcgagat aagtgaaaag aaagatctcc
      421 agtggtataa atactctgtg tatttttgaa aaagatgaaa gaatatatac tactaactat
      481 aattaatcca ctcaatatta ctactttttt atttattttc attcttcttg tttatctcct
      541 ttttagtcac atatcgagaa tctttcaact tattaggtgc aaatcgttta aaacttgaat
      601 acaccactaa acaataagtc agaatactga tccataaagc catgagagca gttggagtat
      661 attttgttgt aatttctatc ttattttctc cagcatttac atccacattc actaagccat
      721 gttttttata agagtcattt acgactttac cgtttacttt tacctgtgta cctaaataag
      781 catatactgg tactgtcact ttttgctttt tgttgcttgc cactgtgtaa gtaatgttat
      841 tatctgcagc tttatacttc cctttttccc acgattctcc tacttttact tcatgttttt
      901 ctaagtgagg cctttctttt atcgcttctg ttggggcgta atcgtataat cctcccatat
      961 ctgatgttgt ataacgtggg agtgtttctt cattgataac aatatgaccg tgttttgatt
     1021 ggataacttt tgcgtaattt tcttttgctg ctatagacgt gacgagtaga aacaccacag
     1081 tagcgccaat gattaaccat gaatattttt tattcatagt ctttttcata aaaacaagcg
     1141 ttatgatcat tgaagaaaaa aagatttgga tacctaagat acgccaagga aactggagta
     1201 agcttatcgg tgttttttga aacaaaaacc atggaaataa attagttacg catacccatg
     1261 tagaaactac gccaacatag atgatttttt cttttttctc catttctttc agtatgaata
     1321 aaggaaaaat caacgcaacc aatcctaata accccatagt gtatgttgta atatcgttat
     1381 ttaacgcttg aatgatacta tctgaaatag aaagtgcttc tagctgcaaa tctgttttga
     1441 agggactgtg tatttcttga taattcatct gctgcaacat aggataccaa aaatagctag
     1501 ttaatgcaca agtcaaaaaa acagccttta ctaaattcac gaccacaaat tttatatcgt
     1561 tttttatttg cttgcttaaa aacaaacgaa ctataatcat acaagcaatc aatgcactac
     1621 acaatagtgt agttaagaca tgagtataca caagtagact tatactgaca gataaagcga
     1681 tgactttttt ttctctattg aatatcaatc cataagcata atataaaaca gctgggataa
     1741 tcgtaagagc gattgcttct gcaattgcgg atcgataata gatatcaact gttcgataag
     1801 ttgcaaaagt atataaacaa ctaaataaaa aagcagcatc tatcttttta aaaaactttt
     1861 ctccataata aaaactaatc agaatcgtac aaatatttaa taaccaaata tatagaacat
     1921 aacttaatat taagttattt gaaattccat aaaaaacgac tgcaggaatt attgtaagaa
     1981 aaggataaaa atagttcagt ccatctccat aattgttaaa tgttgtatag ttgattggcc
     2041 cttcaaaaat agaagataac cctttgattc gtgaaagatg aaaagataaa tcacctgttg
     2101 tataaatttc ttgagcattt ctcataaaat ttactgaata taaagtacta agtaagataa
     2161 gaactactaa aagaaatgct tttttactta ttttcataga tttgttgttc tcctttttta
     2221 attctatttt cagtatacta gaattgtctt tttttaataa gttttgcgtt aaatttttca
     2281 tagcactagt atgatatact agaaaagaat caatttttat tacttttaaa taggagaaaa
     2341 aaatgaaaaa aaacaaaaac caaataatag atatcatttt tatgttattt ctaagttttg
     2401 cttacgtcat ccctctattc atgtctaagg gaatctatca tagtgtcaat caggatactt
     2461 actttcattt atctcgaatc ataggactag ataatgtatg gagttctcca gtaaacttca
     2521 ataacttcga tcatcatgga acaatgatga atatttttta tccatggctt actctttatc
     2581 cagctttttt attttataaa atgatgggaa atcttgtttt aagttataat atctactatt
     2641 tttttatcac ttttttaaca atggtcgttt cttatttttc tatgaaacaa ataaaaaaca
     2701 atcgttatat ttcgctgtta ttttcgatta tttatacatt ttccgcctat cgtgccattg
     2761 acatattcca tcgtgcttca ttgggagaag ctgtcgcttt aacatttttg cctttgatac
     2821 taatggggtg ttacgaaatt tatattagag attaccaaaa atggtactgg ttgtctattg
     2881 gcatgacgct agtagtgtat actcatttac tttctgtcgc tatggtttct gtatttattg
     2941 gaggaacgtt gtttctttct ttttattttt gggaccaaaa aatcgctaga ctcttaagtc
     3001 ttttaaaagc taccgtcctc accttttttt taagtgcggg attcttaatt ccttttattc
     3061 aacaatctag agcacaagaa ttgaaagtcc cactgggaaa agaattaagt ggtatggctc
     3121 ctagtgatat gctgacacat attttaaata ataactataa caactatact attggattat
     3181 tcttatttct cggattgatt ggtgctttta tttttataaa aaaattaact gctgatgatt
     3241 tatttatttt ctttcttggt gtatttgttc ttttttgttc aaccaattta tttccttggc
     3301 aactttttaa ccatacacca gttaagagtc tacaatttgt ctggcggtta aatggttttt
     3361 cttctttatt tatagcctat acgatgagta tagtgatcta ttatattttt tctactaaaa
     3421 ataattcatg gaaaataatg gtctttacgt ttcttgcact aatattgcat ctctcaggag
     3481 taagcaattc tatccatgct aacaaagatt cattaacact tatagcacct gaagacgctg
     3541 ttgctatagc tgaaaattac aatcatactg attatgcaaa caaagagtca atctatcatc
     3601 ctgatatgat taataatgat caatatttac taggtaacca ggaaataaat ccaacaactc
     3661 attttatgag taacaaactt acaattgaat tagataattc gaataataaa aatactgtct
     3721 tgacgacacc tatttatcgt tataaagggc aagtggcttc tattaatggc aaactagtac
     3781 aaacaaaatt atctaagttt ggcacaactg aattaactat tcctccaggt ataaataaag
     3841 tcgttattac ttatcagtac acaaaactag caattgcttc aagatattta tctatcgtta
     3901 ctttgatttt atttttactt tatcgattta ctttttctaa acaaaaacaa ccgagaagag
     3961 aagtacaaca ttctcactag atttttattt aagaaagact ttttagctgt acacataaac
     4021 aggctaaaaa gtcttttttt atatttcttt aatttgcttc atacattatt taacatcgat
     4081 atttatactg tgaaacatgc gtttcatcca ttatcaaagt atttttatcg ataataaact
     4141 tcatccaaaa acagaccttg tgcagggacg gtttctcctg catgttgccg cactttgctt
     4201 tcaaatattt catcgattgt ttctatcggc atggcacctg tgccgatttc tagtaatgtc
     4261 cccatgataa tccgcaccat tttatataag aatccgtctc cgacaaaagt aaaatgaagc
     4321 atttctccct ttcgttcgat agataattca tcaatcgtac gcactgtgga tttttttgat
     4381 tttttcaatg cagaaaaacc aatgaaatca tgtgtaccca ctaatttttc gcaagctgca
     4441 ttcagcttgt ccatatccaa ctcttgaggg aaataaaagc tgtggtttcg ctcaaatgct
     4501 gaaggaatca cgttgttcca aacataatag ctgtattttt tgcccaccgc attatatctt
     4561 gaatggaagc gctcaggcat ttcttcgatt cttttgatga caatatcccg cggaaggtaa
     4621 cgattcataa agtcgatcat ttctgccagt ccaatttctg tgtttgtttt aaaatttgct
     4681 acttgtcctc gcgcatgggt gccagcatct gttcttcctg atccaataat ttctatctct
     4741 tcatttgtca tctgatgcaa tactttttcg atttttcctt gaatggtttt atcgtcatct
     4801 ccaagtcttt gccatcctga atatcttttt ccgtcatatt cgattgtcaa cttgatgttt
     4861 ctcatatttt agtggctcct ctgtcttcca tttggtcttc tctctacctt tttaagattg
     4921 tagcacaaaa tatagctcct tgcttctcta agacagaagc tatacaagaa aactgtggaa
     4981 atatatattt cagaaaaaca aaaaacagcc accggatgaa gtatccgctg actgttcaat
     5041 ttgacggctc aagttttact gtttgagatg caagatactc aaccaggcaa tgggcacata
     5101 attaaagaaa aaactgattc tttggcaaaa acctatttta ttcagtaatg gccaagaatt
     5161 tatagaagga attcttgcca gtccataaat cgccgcaaaa agcaaactga tcacagacag
     5221 tccaaggtat aagtggctta cgtccatttg cccatttttt cgatagatca agaaaagaag
     5281 aactggaaac aataaaaaac cactgtaacc taaacctgat ccgatattat ggacccatgt
     5341 cgagacattc cacgaagatt catgggtatt gatactgaac aaacctgtaa agatacaatc
     5401 acctatacca tagaacgcga tggctgctgt aactagggta gctaacacac gagatgtact
     5461 aacaaatgca gcatacaatg caggcaatga caggatgaaa aagatccctg aaaccacaga
     5521 ccaaataaga aacgcttttc ttacaggact ggaaacctct ccaaatacac tgatcacgct
     5581 cttcatttga tctaattcag gataatacaa tcctaatacg tacggagtca aaaaatcact
     5641 cagcacgcct aataataaaa agaaaaaacc aaatttacgt aaaaaatcca tgatacgcct
     5701 ccaataacaa ttacctatgc ttttaattgt tattataaac gactttatct tgatttaaca
     5761 gaaacctcta tttacacgaa catctatata actcattaaa aactcgaatc attttaaaaa
     5821 tactaaatat ctaacgttca acgttcactt aatccatcta aacaatcttt caatttgatt
     5881 ttttccccac ttcttttacg cgcgcctagc tctcctattg cagtaaacaa cagatcactt
     5941 gacgaattta aggcagtttc cattgaatcc tggataacac cgataataaa tccaataccg
     6001 acgatttgca tcgcgacatc attagagata ccaaacaaac tacatgctaa tggaatcaat
     6061 aacagtgaac cgcccgcaac acctgaagct ccacaagcag ctaaagcaga caaaatacat
     6121 aaaaggactt tcaaaacaaa aggtgcttca atccctaaag aatgtacagc agtcaacgtc
     6181 atcaaggaaa tggtaatcgc ggcaccgccc atattgatcg tagcacctaa cgggatcgat
     6241 atggaataag actcttttgt caatttcaat ctttcagcca acatcatgtt tatcgggata
     6301 tttgcggcag aacttcgggt aaagaaagcg ggaagtgcgc tttctttcaa acaaaagaaa
     6361 atcaatggat agggattttc tttaataaac aaaaacgtga ggaatgggta cacaactaat
     6421 gcgacaaaca gcatcgtacc gatcaaaaca agtaaaagct gcagatactc tgccaatcca
     6481 gcgattccag tcgtagcaat tgattgataa accaatccta aaatccctat cggagcaaat
     6541 gcaataatca cttgtacgac ttgtgacaac gctatagata tctgatccac caactctttt
     6601 gtcgattctg aacgtgctcg aaaagctagt cccaacaata cagcccagaa taagacgcca
     6661 aggtaattcg catcaataag agattgaaca ggattttgtg tgacattcgt caacatagta
     6721 gataaaacac tttccaaact ttgcggtgct ttttcagata ctgtttcttg taaagtaata
     6781 ggaacagtaa aaattgaact tacaataact gctacaagtg cagaaagcaa tgttcccact
     6841 aaatataaga tcaaaatagg cttcacgaat gtcttattcc caattttatg cttggcaatt
     6901 gacgccatcg tcaagaaaaa aaccagaata ggtgcgattg ccttcaagca actgacaaac
     6961 aacgttccca atattgaaat aaatgtccat gtggggaaaa agataccaaa caatattccg
     7021 actaaaatac caacaacaat acgttgaact aacgacatat ccaaaaactt ctctaacaaa
     7081 tgaccaccct ttcacaattt taacaatcat tgtatcattt tatgcaaaga aactaaaggt
     7141 ataattttct aaaaattcta acaatacaaa caaccatttc ttgctaattt tgtacttttt
     7201 ctgtctacac gttgtttttt gagatattct aagttaggat gttattaatg ggcaacttat
     7261 caggaaacgt tttcttatgt ttttgtccaa tttttttgtg aggtgtttat tcttgaaaaa
     7321 aataaagaag gagagttatg gcttcctgac aatctttgtc gcttgtataa tgatggtttt
     7381 acagcgtata ttgatttcag cagtcactat tcctacatat gcaacaaacc ctggtcgatt
     7441 ctacatctat acatttttac aaacttccgt tgttattggt gcaaatttga tcccacttta
     7501 catgggttac caatatcaaa aaatcgcccg tttaaaagtt ttacattatt taagtcgttt
     7561 tgcctttata ttcctaatag ccacactagt ttgcaatatc tttttttacg tacaagctgg
     7621 cagcttgaat attcgtgatt attggcttat cctaacacct atcagtcaga attactttac
     7681 ttatgctgtt tcttgtactt tgcttttttg tagtattcct tatacagttg gatttttaaa
     7741 taaattatcg aaaacaacaa ttaaatatct actattattt ttaacaattg gattagttgg
     7801 ctgctctact ctatttaata aagacccgtt tgcattacag aatggacaga gtattttatg
     7861 gattttttat ttatttttaa tgggttatgg tttgaaagaa tggaactgga aagaaaaggt
     7921 ccgctataag cttcctcaag tatttatcag cttgattgtt ttattcggtt tgattatttt
     7981 gatgacacaa atttcactca tcatcagagg aaatacagat acagcgctga ggttttctaa
     8041 accatattct ctcttcgcaa tgtattatac tttctcttcg tttttatttt tagaaatgct
     8101 atctgataaa ttaggtataa aaggaagagg aaattttctt gctgtatttt tgattgtcac
     8161 tcaagttata tgtaatttcc ctttagtttc ttatcgcgta tctacattta tgaaacgtga
     8221 atttccaaac tcagggaagg catggctgat aaatatcggg cagtttttta tttgatattt
     8281 attagcagtg tccctcctag ttatcttttt attttatata caaaaaatcc gtttcattca
     8341 tcgtttcatc gaatacttcg cttttgattc gattgaacaa ttaactcagc gttggaaaaa
     8401 aagaattcat tggctttttg ttcataaaag ttatttttta gttgcagcat ttttttactg
     8461 ctttaccttc gtgcagattt tcgttttaga accaaaagaa ggttggaaag aaactatcca
     8521 ggttgcctta aaaatattta ctcagcgaca agcacccatg atcctgacaa tcatcattat
     8581 tatgggattt ttcttcttac tattgttgat ttccaatcgt ttttggtacg ccttgactgc
     8641 taccttgatc attaacttac tgctcactat ttcgacagta atcaaaatgg aaatgcggga
     8701 agagcctgtc tttccttcag atttaaaaat gctgaccgga ttgtcagaac tattatcgat
     8761 ggttagccca gtcttattaa tcgtgggcgg attgatcctt ctcctattat tgattactag
     8821 tattattgta caacgtagac tacaaaaaca gtattccttg aagattcatt ggaaacgtcg
     8881 atttatcagt atcgctgtat tattaggatg tttttcgggt gtctttttca ttaatcacaa
     8941 aaattcgcct tctttcttgc tgtttaattt atttaaagta aacaagactt tctttaacca
     9001 gcaaggcgcc gtaaaagata acggaccagt cattcaattc ctaaacaata tcgacattaa
     9061 aatcatggaa gaacctgctg gctattcgaa agaaaaaatt aatggaataa tgaaaaagta
     9121 caacacaaag gcagatgaaa tcaaccaaac aagaagtgac tggttaaaca atcagacgat
     9181 cgttctcaat ttaagtgaaa gcttcagtga tcctgcacgt gtccctaatc tgacagtacc
     9241 tacagaccct attccttaca tcaaaagcat tataacagaa ggaacaggag gattgatgtt
     9301 atccgtcggt tatggtggcg gaacagctaa catggaatgg gaaggactga caggcttgag
     9361 tatcagtaac ctgtctgctt cgttagttac accttatacc cagctagtgg agcgtcagaa
     9421 aataagtcca aatattaccg atctattcga tgaaaaaata gccatccatc cttttacagc
     9481 tgctctttat cgcagaaaac aagtgtttga taaatttggt tttgacaggt tctattattt
     9541 agacagtccg gataaattaa cttatacgga taaaatagaa caaagcccac gcatatcgga
     9601 tgattcagca tacaaagaaa ccttgaaagc tttacaaagc aatacggaaa cgtcacaatt
     9661 catccaatta tccactatgc aaaaccatat gccttatgat gattactata gtgaattgaa
     9721 ctacactgct gaaggaactg ctgtcattcc taatagaaga aaagaattac aaacttatat
     9781 gcaaggcctt cactataccg atacagcagt aaaaaaattc atccaagaaa tcgacaaaat
     9841 cgataaaccg attacctttg ttttttatgg tgaccattta cccgcaatct atagtgggaa
     9901 tgattcgaaa aaatatggat tagaacagca ccagacagat tatttcattt ataacaacaa
     9961 gtatagtcgt gaacaagcag ctacgttaaa taaagaagtt gttgctccta ataactttgg
    10021 tgctatggct cttgagcatg cgaatatcaa agtaactcct tactatgcgt tgttgacaga
    10081 agtatctaat catctacctg cattaacaat tgatcctaca gaaagcttgt ctaatcgttt
    10141 taatggaaaa caggtctttg ttacagagga tagtcaaatt atccaagaaa aaaatttaac
    10201 tgagaaacaa aaagaaattc tagaagatta tcgtctgata caatatgatc ttgttgcagg
    10261 taaccagtac agtgcaacat gggcgacaca aaaaataaaa gactaacaaa taaactgagc
    10321 aaggggttgt gataaaagac atgtcacaac cccttgttta tctcattaat ttgcttgtcc
    10381 ataaacgatt tgattaaagc catttttcaa ttcattattc gctgtctcgt tgaagtaaag
    10441 ataattatcg ggattcccct gattttgttg tgtcaccaat acttttggct gattttgctg
    10501 aaacccgaat agatagacgt gctttttcag atatggttct gtctctgcat cggaatagac
    10561 ggctgtgatt tgatagtctg cttgacctgt cccactttct gaccattctg cagaaacagg
    10621 cactccacca atagccatct tccactcccc gttcaaaata gcttgaggga ctttaagacc
    10681 atataagtcc acactcattt gattcgtata acttttgtat tgttgtccca ttgtttggcc
    10741 tcatttggtc ataaatgcct ctaattgtgc ggatttgtct gcattccaca gtgtttgttc
    10801 aacttgttcg ctgctttctg aagaggaaga tgaagcctct acagaagaac ttgaagtaga
    10861 tgtttgagtt gtttcagtcg ttttggaaga ctctgacgaa gtagaggtat ctgctgtttc
    10921 tgtacttgct gcagttgact tgctctctgt gctagttgtt gtagtagttg caactgttgc
    10981 tttcgctgtt tcttttgttg attctttttt cgattcattc ccgttggtac aaccagctaa
    11041 caaaaaagct agaccaattg ttactaaaat cctttttttc aattaaaaaa ctcctttcga
    11101 ttactcccac ttctttattc tatcaaataa gcaatgtgat tttattgatt tttcgtattt
    11161 tctcatcaat ctttcaaatc ttctaaaaac tgagaagcac ccgctgatgt tgtctgtaat
    11221 acatcagcgg gtgcttcttt tattttaatc ttcgtgattt tccaatttac ctcacgcttt
    11281 atatttgtat ttgaagaaac atagactttt agtcatcatc gtcttcctca tccaaatctt
    11341 gtcggacagt ttttccttgg agtctttgta cttggtagat gatttcttta ttgaaactta
    11401 aaagttcaac aagcaattgt tgcaagccat atttttcttc tttttcagct aatttgattg
    11461 cccgtgtgat aaagacattt tggaaatcaa aatcttttgc aaagttttcc aacattgttg
    11521 aggctggtaa atatttgttt tgaccatgtt cgtaaatttt tccataacgc atgacttctt
    11581 ctaaagtaga cggaacgatt tcattttcaa tcactaactc atgagagatt ttattgaacc
    11641 atctcgcttc ttgatcagca tattctgaaa aaagggtact taattcttgc gaagatgtac
    11701 ccaaaacata caattgtgct tggatcaatt tcatttcttc taatttaaaa tttgctaata
    11761 catgccccac catggcgcca gcagttggtt tatgatggtc gatatctgct tgttttgttt
    11821 cttgaacaaa tttttcttcc actatattca ttgatttacg cctcttttac ttttgctgat
    11881 tggacttcat aacctaattt ttccacaact gcagctaatt gttcagggtg gacttcatct
    11941 ggatcaaagt ttgctttgat tttgctcgca ttaaacaaaa ctttcgtatt gcttacccca
    12001 ggctgttgag aaaccgcatg ttcgattttt tgcatacagc ttggacaagc tagtgtatct
    12061 aattgcataa taattttttt catcgtagtt cttccttcct ttttcttgat agtcttattc
    12121 tagattacag cttccataaa aaaattgatt cttatcaagt tttacgattt ttcttctttg
    12181 ctttcgtgtc caaaatacaa taaacgcatc gcgttaaaga taacgatcaa gatactagct
    12241 tcgtgaacga acatcccact agccatgtaa ataaacccgg caaataagcc gttgagtaaa
    12301 aataagacga caccaatggc aatcactaca ttttcacggg tatttaaaac agttttctta
    12361 gccaagcggt aggcatgaac caacgattca aaattagatt gcatcaagac aacgtcactc
    12421 gtttcgatag caacatccgt tcctgatccc atagcaatcc cgatatcagc cgcagccaaa
    12481 gagggagaat cattgattcc atctccaata aaagcaacgc gcaatccttg ttcttggtat
    12541 tttttcacaa attgaacctt ttcgtctggt aataattcag catggacttc atcaatgcct
    12601 aacatatcag ctacataatc agcagtcatc tgattatcac ctgtcagcat cactaactgt
    12661 tttgctcctg cttgtttcaa cttagctaat tgttcagcga cttctggtcg aatcacatct
    12721 gacacaccta tgatttgagt gatttttttg tcgattgcta caataatgac cgagctgcct
    12781 gtttgttgta attgatgtaa atcttgtact tgctttggtg tgagagaaag tttgtgagcg
    12841 gcaatcaatt tggtgttgcc cgcacaaact gtatgaccat caatctcagc tgtgattcct
    12901 tgtcccttga ttgtctgatt atctttgact gctaattgtt gaaaatcaat gtctttctcc
    12961 tgagcataat ggatcacggc gcgccccaga ggatgatcgg ataacgtttc taatcgagcc
    13021 actaaagcta agctgtcccc ttcatcctct gtatagttct tgatagtgga tacagcagta
    13081 gttcctttgg tcaatgtacc agttttatca aagacaaaag tatcgatttt ggaaaaagta
    13141 tccattgctt caccgccttt gactaagatg ccccgtttcg ctccattacc gattcctgct
    13201 acattcgaaa caggcgcacc aatgactaaa gcgccaggac aaccaagaac caaaacagtg
    13261 attgctaaac gaaaatcacg actaatcaga ccaacaacca acgcaataag taaaacggtt
    13321 ggagtataat actgagaaaa tcgatcaatg aatttttccg catgggattt ggtatcttgt
    13381 gcttcttcta ctaattcgat aattttggca aaggtcgtat catctccgac cttagttgct
    13441 tcaatcgtca cgtagccatc tgctaaaatc gttccagaga agacttcatc tccactgatt
    13501 ttgacgactt gtcgtgattc accagtcact gcggcttcat ccgcataccc tttcccgtca
    13561 atgactcgtc cgtccacagg aatagctcca ccagttttta ctaagacgat atcgccttct
    13621 tccacgtcat cgatttccac ttcttcagtc gttccatctt cttggacaac actagcagta
    13681 gatggggcca tctcggtcaa agccttgatt gactcccgcg ttttggtcaa tgttttttgt
    13741 tccagatagc taccaaacaa aaacaagaat gtaacgatag ctgactcctt gtattctccg
    13801 atcataaagg caccaagcac agcaatagta acgagtaaat caattgagat aactttgact
    13861 ttcactgcct gatacgcatg gatcataata ggtatgaccg caataataga agctatggct
    13921 agagcaatgt tatagaacaa gacaaaacca gtaaattttc cgataaaacc aaaaataatc
    13981 aacacagctg ataaggcggt gatatgattt cgatgctctt ggatccactt ttgtaatttc
    14041 atctttctcc ctcttttcct ttcattctta cgagactaag tatagatact tatccatcat
    14101 aaaaaattga ccactatcaa gtttagtcaa aaaggcggtt ttcttttggt agaatacact
    14161 acaaaaaata gttagcaaga agactcacta aaagtgatct tgccaactat tgtcgtttct
    14221 atcgaaattt tcattgaatg attttcctgt acagcataca tcggagatct atttctttat
    14281 ctaagatttt tatcttgcta attttttcct ttagtctttc aaatccacgt cgttcataga
    14341 attgatacgt acagccacta tctgtaaaaa ggaatatttc ttttcctttc tctctttttt
    14401 caaattctgt tagcaacttg gtaccgatgc cttttgtttt caaatcagga tttgcagcta
    14461 aaaaaatgat ctctccatct ggcgaatgct tctttaaata tgctgcaagc atttgttcat
    14521 ttgctgtctc gtaagctcct ttactttctt tagcaaatat attttgtaaa aaatcgaata
    14581 tcttaacata tatttgctgt ccaaaagaac gatgtttctt tttttctcct tttatctccg
    14641 ccagcaaaac acctgccaac tcgtcctctg catacaaagc aagaatttga gttgctcgat
    14701 tcaactccat atacaaaaaa tatcttccgt aaagattcag cagaagttca ttttccatat
    14761 atctctcaaa gttcattcct tcaacagcaa aatagatagc ttttttgtaa tctttctttt
    14821 ctaagggttt aaaagttagt ttcatcattt cgcctcctca acttcaatga ttgtgttata
    14881 cttacattat aatagacaca gtgtctacta tctataatca atcatctata agtttgtcga
    14941 ttaagcgaca gctttgcttt gatatgtcgg ataaggagat aggtaaatga aaaagacaga
    15001 tagacgcgtc aaaaaaacag aaaaagcttt ggctgaaaca ctatccacat tattagtaaa
    15061 taaaaagatt caagcaatca ctatacgcga actaacagaa acggcagatg tacatcgttc
    15121 gactttttat acacattata aggatatcta tgatctatat gatcaattag aaagcaactt
    15181 ttttcgggat ctaaatgaaa ttctctctta tgatccagcc catagctatg aggaactata
    15241 cactcgcttg attgactatg tttacgcaaa tactttttta tatcgcttat ttatcctcaa
    15301 ccatggtgat atttcgtttc agaagaaagt taaacaagtt attgaagaaa actatctgaa
    15361 catatggcta tatgaagaca agaaaacgat tatcacagaa gaaatgagat tctttactac
    15421 gtaccacgtc caaggttgtc tatccgttct caatcggtgg gtagaaagca attacaagta
    15481 ctccaaaaaa gaattattag aactcttaca gaaactgaat gatcacatag agaaaatcat
    15541 gccttgaata aaaacatact aagcttagtt aagaagaacc gtaaccttct cgtataacaa
    15601 ctgaatctta tttagccaaa ttgtttactg aagatttttt gagtaaaatt tctataattc
    15661 ctacttattg aaaagtagtt tgtagtgctg cttctccctc tctaagtgat atatcttgtt
    15721 gtaccaatca aattcacctc atcattaata aattactttt aggctaattt attacaactt
    15781 ataggttgaa gctgtaacca atttatagtc atattatatt tttatcatcc agtttattga
    15841 attcttctcg tttacctcaa ttcattttca aggggaatca gctcatcatc taacatttta
    15901 cgccatgctc gcttcatttc ttgtgggtat ttaaatggtt tcgcttctgg atgtttggct
    15961 ttatatcctt cgtaatcctt actgttgata attgctttca tattaggaat tggatcttca
    16021 cctggcatat attgcagatt agaagcatgt aattttttct cagaaacaaa ccactcattg
    16081 gaaaagacat gaatcaaatc atcacttact tgtgtaaaat agcgacgttt aatgacaaag
    16141 atatcttcat tgctatctag tgtaccttca tttctttgtg cttaaattca aagataattt
    16201 tctcaataaa gatttttgat tccagtaaga ttcctttact ccttttacaa catagcgttg
    16261 tcctttgttt tgaaacatct tcttcccctc tttgccatta aaaatagccc aattctctat
    16321 aaagaagatt aagcttcatt attataatat aaagtttaaa tttggtttat tacttacttt
    16381 ttcttattct ctagtaagct aatattgaaa aaaatgtaat cagatcatta tttttatatg
    16441 ttgtaacttc ctgataagaa aactaattaa aagaaagagg taactacata aactaattaa
    16501 aaaagctaac ttcatagttg gaggataaat ggaagaaact tcaatgattg tttctccttg
    16561 tgttccttca atcgacacta gtcctattat caatctctct tttttgtcca tttacttttg
    16621 cgactgttcc ccaataatca aatacaggaa gcgttatttt ttgtccttta gattcatgta
    16681 ctgtatagac tattttcgaa tccatcactt gataaggcat agtatgccat tccttgccaa
    16741 ttcttacttc atgattctgc aaatgttctt tgtattgtaa tgcttccgtg ggtgcatagt
    16801 cataaagtcc tccgggaagg agtgtcgtgt aacgaataag attttcttga ttcattacca
    16861 agtgatctct ctgagtgaca actttatgtt catagtttgc ttttgttgca gcagatacag
    16921 ccaacagtaa taaaacaatt gccgttatat taactacttt tctttgctta gctgatgtgc
    16981 tcttcatgaa aacgaacgat aaaatcaacg agccaaacag tacttgaaag cctaagattc
    17041 gccaaggaaa ttgaatcatt tgaatgggtg tattttgtaa taaagaccat ggaaaaaatt
    17101 tggtggataa gagccaagag actaccgcac taaggtaaat cactttttct ttgcgattaa
    17161 atcctttaaa aaagaagagt ggacagatca aagaaataat accaatgatt cctaccgtaa
    17221 aggttgtcag gtcattattc atcgcaccaa tcaaactatc cgagacattt aatgcttggt
    17281 gttgtaaggt cgttttatct ggctcattaa tcgattgaac catgatttgt cggaacatcg
    17341 gataccaaaa gtatgctgtt aaaagaatcg tcattcctac tgcctttgac aactccacaa
    17401 tcaaccggat gctttctttt tttcttgttt cacttactac aaagccgcct aagactagta
    17461 agccaacggc tattacactc ataaaagtgg aaagaacatg tgtataaatg agtaagctca
    17521 tactgactgc taatggaatt gcacatggct ttttctcata aatcagtgcg taagcataat
    17581 acataactat cggtataacc gtaagtgcaa tcgcttcagc aatagcagaa cgatgataaa
    17641 tatcaatcgt acggtaccca taaaatgtat ataaacagct aaatataaaa gccgcatcga
    17701 ttctttttaa aaattttagt ccataatgaa acatcagcat gatagtacaa acattcaata
    17761 gccaaacata taagatataa ctaacgatta aattattact tatccaataa aaaatgactg
    17821 ccggaaaaaa ggtcaagtag ggataaaaat aattcactcc gctcccataa tgattaaacg
    17881 tcgtaaaatt gattggacca gaaaaaattg aagataaccc tttgactcta gcaaaatgga
    17941 aggcaatatc ccccgatgta gaaatagtat gaacatttct aaaataattg attgaataaa
    18001 gtccagcaat aattgctagg agaatggaca atattatttt tttctttact tgcattactt
    18061 aattatatac tcctttcatg gattgcttta tctagtataa cttaaatttt aaataaatcg
    18121 ttatttattt ttactctcac ttaaaaaagc actaaaatta taagagaggg aggtaatggt
    18181 ctgaacacca agaattgttc caacatcgaa ttgcttaact aatgagttca aagcttaatt
    18241 atgtggctct tatatttacc gagcgtggat tgcgatctct ttagcgaaac aaacatcttt
    18301 cataaaaaaa acagccatat ccgctttctt ggatatgact atttactact actttactat
    18361 ctttcattct tcttgtctca cttatgataa ggcgctgggt atcatgagtg aatgatattg
    18421 ctgaatacaa atagtccgac cacattaagt tgatcggact tcataagcat tttattgact
    18481 aatacttttt tgcttaagaa ttgattctaa catcttttgc taaagttaag catcctatag
    18541 ttcctgcatt atctgctaac ttaggtgtga caatgtattc ttcgatattt ggcgtatcga
    18601 catagccgtt aattagttct aaaaaacgtc tttgtacttt ctttttcaga tgttcttgtt
    18661 gcatgacacc accaccaaaa attataatat ctggtgaaag catcagggtc gtattacagg
    18721 cacattgtgc aatataatcc gcctcgattt cccagaacgg atcttctata gaaagatctt
    18781 gtcccttggt tcccgtacgc atctcgatag cgggtccaga tgccaaccct tcgagacaat
    18841 catgatgaaa aggacaagta ccagagaaag tatctcctga atgtctctta actaacgtat
    18901 gccccatttc tggatggcta aacccttcaa caaaaatccc atcttgtata gctcctccac
    18961 caattccagt tccaacagtg taataaacaa cactggataa accttttcca tgacctgaaa
    19021 catactcccc gtaacaagca gcattcacat cggttgtcca ggcaattgga atttcgaaat
    19081 gcttattcaa cgttccaaca aaatcaaaat ttttccaggc agtttttgga gtagaggtga
    19141 tatgtccata ggtctttgat tcacgatgga tatcaatcgg tccaaatgac cctactccaa
    19201 tgctttctaa ttgttcttta tagtgattaa agaaatcaat taccaaactc attgtttcat
    19261 tcggtgtagt tgtaggaaaa ctcactcttt ctaaaacctt taaatcatca ttgccaatgg
    19321 cacaaacaaa tttagtccca cctgcttcta ttgagccata catttcttac tccaccgtcc
    19381 ttttatctag gaattcgttc ttccctgtct aatagttttt cgaaaaactc atgtggttcc
    19441 ttttggtacc aataggcaac agaagaaata tcgtcttgac gctcataaag cttgatgtca
    19501 tcatttccta gggtttggaa agtaacacgt aaattttctt tgaaactaat ggggtcgggt
    19561 aaatgccagc ggtacaaccc atgcatcggt aaagcatcat cgccaaaacc atgaactgga
    19621 tttggattag gttttccagc actaaaataa tcccgggtgt gatctctttt agattgaaaa
    19681 ggatagccca taaatagcgt attaaaattt tgagcatatg ccctttctcc aaagttcttt
    19741 ttatggaaag cccaagcgcc gccaaaataa tcttctgctc ctgtagatgt tattgtaggg
    19801 aaatcttcat ctccatctat ataaaactta aattctcctt ccccccacca gtatctttcc
    19861 aatgcgcaca aagcaaagta ggttcctaca taatacccag tgcctttgat tccatcaata
    19921 agggtgtaat cttttccttt tgtcgtgatg ggttcacggt tccatttagc atgaaaatac
    19981 atcgcatcat ccggtacttc agtcaatgca taattaaatg tataaaaaat gtgcttaata
    20041 aactcaggat gttcattcgt taaagtaatc ttagcttttt ttcgaaatgg catctcaaaa
    20101 tagcaattca tccctgatgt tggattcact acgattggta aggaattcac ttcgcatctt
    20161 gtcccaaacc cattacaaaa aaaatctcct aatggacttt ctacagaagg agtggattca
    20221 tcatcccaat aaatacgcaa aattaaatca cgtaagacga aactcccagc gattgttttg
    20281 tctgtcactg tcatccaaaa atgacgtaaa attccagggc cttccatttc tgctaaagta
    20341 attgtctctc cagcatttat cggaacagaa ggtgaccctt ttctcccaat tcctagatga
    20401 ctcgctgtca ttgccgcttt ccctttttca ccatttttat tttctggtga aatagttcta
    20461 ctttcctctc ccttgtggag ccatatatta tttaaacaat tcattttatt cgtcctctat
    20521 cctttcttca cttttttgat tattcatata gatgatttgt tgttgtgcct ccgcaataat
    20581 tgctccaata tctaaattat catttcctaa ctgcaaagcg attgacattg ccaagggaag
    20641 attcattcct gcaattacgt gcactctgtc agaaatatag gtaaaaaact tttgatttac
    20701 acttcctcca gccatgtcag ttaaaacaat tatttcgtct gtagtatcca ctaactcttt
    20761 aatttgttca tctatatcat caatttcttc gacatatgca cataagctac taatttgatt
    20821 atgcatacct gttaaaaact ctagtgtctc tttaaaaccc tcagctagta ttgaatggct
    20881 agcaatgatc aatcttctca taattcaaat ccttttcctt tacgctaata ttccaaatgc
    20941 agccataacc atcgcaaaaa caataacgag tagaatcaaa atggtcatac tgattttttt
    21001 cttactgagt agataatatg ctgcagcagt aataattact ggaattaatg ttggtaaaat
    21061 tttgtccaac attggttgga ttttcatact aacttcacca aaagtgaatt taagtggcgt
    21121 tgaaacactg ataactgaag caattaatga tccaactact gttagcccca atactgaagc
    21181 agcttcagtt aaataattaa tcttttcacc gtattcagta acaatcttca ttccctgctt
    21241 ataaccaaat tcaaagcctc tcattcggaa aaagaagatt accatgatta aaatgaacca
    21301 taaaaagatt cctacaggat tattttctaa agccatataa gccgcaattg atcccatgat
    21361 ggttgggatc atcgtcatca aaagagagtc cccaatacca gcaattggac ccatcgtacc
    21421 aactttaaag tcattaatcg catcttttgc ttctaatcct tgcttttcct ccattgcaac
    21481 acaagcacct aaaataatgg ctgccagcca aggctgtgta ttgtaatact gaaagtgatt
    21541 atttaaagct tcgatgtatt catcatcatc tttgtataat tttcttaaaa taggtgacaa
    21601 tgcgtaagtc acagatgcac cttgctgtgt ttgataatta aaggtacaaa ctgacatcaa
    21661 ccaacgatag ttcgctttcc taagatcctt tttcgttact tgatattttt cgctcatttt
    21721 attcatcctc cccaataaac ccaacatcag aagctcccgc agaagcagtg atttgtgcat
    21781 ctttttcagc tctatgatac acattaaatg ctaaagcgaa tcctatgatc gccgctccaa
    21841 taataggtac cttcaaaaaa gcggctaaga aaaatccaac aagtaaataa ctaatgtatc
    21901 gacctgtagg cataaagcgt aacaacatcg caataccaac agcaggcaac atttttccgg
    21961 ctaatgttag cccatccata aaccaattag gtaaaacatc caaaacgtaa ttaacagcag
    22021 actgtcctaa agtaacggca ataaatactg gaattgctgc agataaacca aagaaaattg
    22081 gccccaacca caaaacacta ttcattttct taaattctcg tttattcgca tattcctgtg
    22141 atttgcgaac aacaaaacca tttaagattt tgacaattac atctagttgc acccctaaca
    22201 tacctacagg tagtcctatt gctaatccgg cttctactcc aagctggcta ccaaatgccg
    22261 ttgcaataat tgcgcacgtt ccatagtccg gtacagatga gccaccaaaa ccggcaactc
    22321 ctaatgacat caattgaact gtaccgccaa ctatcaaacc tgtttttata tcacccatca
    22381 aaattcctgt gaataatccc caaaaaactg cgtaatttac aaagatcatc ggaatcccat
    22441 aataatcaac atgtttaata aatgctaata tagtcagcat aataatttga aaaatagtaa
    22501 aatgcattat ccttactcct ttatcctaat tttgattgaa tttcctgtgc cttatccgcc
    22561 ggaacaaatt gtgtaacaat ttctacacca gaatccaaca attttttata cgtgctgata
    22621 ttttcatctg atgcataggc tctagaagat agctgtaatg cattatcttt aacaacagta
    22681 ccgccaacaa ttagtcggtc aattctaata ccgttatcta ctaaatgaag gattgtttcc
    22741 ggctctttca cgatgacaaa tactttttcc tgatcgtatt tccccttttt aaaattcgca
    22801 atcgctgttt cttcagtgat aattgataca gccattccag caggtctagc taaacgcata
    22861 cttgattttt ggatgtcgtt atttgcgaca gaatcatcga tcaccatcac tctgtttgct
    22921 ttagacattg gtgcccattg cgtgacaata atcccatgca ataaacggtt atcaactctc
    22981 gacataacaa tactcataaa tacttcctcc tttatctttc attttcttac aagcaattac
    23041 catgccaaaa caaaaagaac ttgataaatc aagctctttt tacataatca taaacgtatt
    23101 taacctcatg aatcgggatg tcgacactat aataatgttc aacgacactg aaagattgct
    23161 taaaatgtgt ctgaaatttt tgaaattcct cactcgtttc attgaaataa atttcatctt
    23221 ccaaagtatt ttgttttact aatcgttcaa ttaagcaact aatgtgaaca tataatccga
    23281 aacacgtatt attatcaaat gaaattccta gatcattttg taagatgtat atagcatctg
    23341 aaacccgttc taataattta gtcggattta atatggtcaa ttgctccatt aaattcgtta
    23401 gactaaaatt cttcagaata ttattgttca aaactttcag tacattatca ttaaagtgtc
    23461 tattcaattg attggagaat ttacttatat tcattcctaa aatcatatct tcaattgaaa
    23521 agaattctac atcttctaga tgtggattta gtgttccaac aataaaaaga atattgttct
    23581 ctttacgaag ctccgataaa tagttttcat tcactaaaga gtaatagtca cacgtttcta
    23641 tgacaagatc agtatccctt ggaaagctat cttcaagtat cctttttaat ttaaacgaag
    23701 ttcctaatcc gcttgcacaa gaacagacaa tcatatcctt cttaatattt gatgattcat
    23761 aaatataatc cacattttga ttttctttta cttgttcaag aatctgttta agcggtaaat
    23821 ctaataatat ctgatttgca acatcaatag ccattcttgt tgaaacattg tttagtagtc
    23881 cgaatttttt ttgagcgtct agttccaaat ttagataaat tttctccagt gaacccatat
    23941 caacaagtaa aataatttct tcatatccgg tacactcttg taagtatcta ttaattttgt
    24001 ctgcaatctc actacttgaa gtcattagtg gcatatcaat ggcgtcgtat atatatctct
    24061 caacgatcct attagcagta cttgctatac aggttgccgt tagatctcca tgagccaaaa
    24121 tcagtgctaa acgtttttgc tgaaattctt tactacacat taacttaaat aaaataacca
    24181 aaacattttt ttgaattttt gttaaataaa tatcccctat ttgaataaaa cgattgacta
    24241 attgaacttc tcgactacac caactattaa tatcattgat gaaggaatat aatttatcgt
    24301 ccgatgttaa ttggatttga taaatccaag aattcaaaaa gatagacgtt atttgaagat
    24361 aataatagtc aaattctatt tgaaaatctc tctctatttc cttaacatac
//
LOCUS       DAZKLD010000028        23936 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.028, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000028 DAZKLD010000000
VERSION     DAZKLD010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23936)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23936)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23936
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.028"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(53..787)
                     /locus_tag="ACG8KR_000933"
     CDS             complement(53..787)
                     /locus_tag="ACG8KR_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HFQ0200325.1"
                     /translation="MDYSLMTRLAAEMIGTALLIVLGNGAVANVDLKGTKGYGSDWML
                     IAVGYGFGVMMPAMIFGGISGNHINPAFTIALASNGLFPWAEVVPYIIAQLIGAAIGQ
                     LIVVACYKPYYDQTEDVNHVLGTFSTINSVGSKFNGFVNEFFGSFVLFFGALGITKAP
                     FFQDNLGTAHLALGFLVWTLVASLGGPTGPGLNPARDFAPRLVHALLPLKHKGSSQWG
                     YAWVPVVAPICAGVVAVFLYKLLFMA"
     gene            1114..1524
                     /locus_tag="ACG8KR_000934"
     CDS             1114..1524
                     /locus_tag="ACG8KR_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HFQ0200326.1"
                     /translation="MKFFQEFFIIIFFSVLGEGIRIFAHLPIPGSILGILFLFLAFEM
                     HLVDPEKIGATGDFLLNNLAILFVPAGVGLLEYFDDIASIWPVLLGAVVVCSIVTMVA
                     AGKTAEGVEALLGYVRKKKAERVSVNEMEENQID"
     gene            1517..2218
                     /locus_tag="ACG8KR_000935"
     CDS             1517..2218
                     /locus_tag="ACG8KR_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HFQ0200327.1"
                     /translation="MTEFFVNPLFGLSLTLGVYVLSDKFLRKYKLPVLNPLVLSIVVI
                     ILFLHFSGISYKDYNIGGNFLTMLITPATVALAIPLYKNFRLLKENFYPVMAAILAGI
                     VANCLVSVGFGYFFSLHKEMVISLLPKSVTTAISVDLSHTMGGVNAVTLAIVVSTGIF
                     GSLIATHIFRLFKIESPVARGVALGSTSHAIGTAKAIEIGEIEGIISGLAICVNGILT
                     VLLLPLFFQPFAGLF"
     gene            2218..3804
                     /locus_tag="ACG8KR_000936"
     CDS             2218..3804
                     /locus_tag="ACG8KR_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0200328.1"
                     /translation="MRKPLFYATITKTFEHKKGGKDVYLSSEYLKQFDKDLYYKILLL
                     ESFEDQAWHTAAQLAQVVQLDARSVSKYLNELSKNYQQFSGKTHPLFTKNHRSGYNFY
                     DTLDSIEHERFLIYLVQSTLKFQLLHDIFFEEFHTMYQFAQKHYISESTAHRKINEWK
                     QQLQTYGIRLQRGTYIAQGEEEIIRLYLHMTFWQLFRGKIWPFETISQMDVKNMAEHI
                     MAFFNVRLNEIKKRRLEYMLGAFFLRKSQKHYVVLNEKKRRLISDNLLFQRFCQVMEP
                     VFPNYFQVEDELGALFLVLMTREEYYSDPKIRKKIFDFHQQAKTPPFTALSEAKAALS
                     LYQEEQGLPAENLTFEAENYLFSSHFFAYLFPNAKETIDGNSSDFINHLVIENKELKQ
                     WLVHFFESRHKHPNHLAFKNHAFLMGRYLTVFKTLGAFTPQLPKITILLMTDFPLFEE
                     QLLEEGLRTFFRNEYQLIFLPTDYRGREVDLLISTSKVHRKPWADLDYFIVTEELKLI
                     DYIQLSQKFEMIQKQKLLKQ"
     gene            3823..4044
                     /locus_tag="ACG8KR_000937"
     CDS             3823..4044
                     /locus_tag="ACG8KR_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HFQ0200329.1"
                     /translation="MTFEEILPELKKGAKIIRKGWSGFELYVVLVSDDEFDGCPVTPY
                     LLIKTSDEGFSSFAPTVCDILADDWAIVE"
     gene            4041..4802
                     /locus_tag="ACG8KR_000938"
     CDS             4041..4802
                     /locus_tag="ACG8KR_000938"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HFQ0200330.1"
                     /translation="MKNQYPDLAKQVVFITGAASGIGAAQARAFLEQDAFVFGLDQKF
                     GKMEEISLRFPETFAYALGDVRQMKELQQAVSKCQNHFGEVTILLNTAGILDAYKPLM
                     ETDESLWDLIYETNVKSMYQLTKIILPDMIRQKSGTIINMASIAGLIAGGGGIAYTSA
                     KHAIVGFTKQLALDYAGQGIRVKGIAPGAIQTPMNAADFEGKGEMAEWVANETPVKRW
                     AQPEEVAELTLFLASPQASYIQGTIIPIDGGWLLK"
     gene            5182..5691
                     /locus_tag="ACG8KR_000939"
     CDS             5182..5691
                     /locus_tag="ACG8KR_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HFQ0200331.1"
                     /translation="MEKNNTRSAKRWTTTDVTRMALVTGLYVAVTLVLSVFSFGVIQI
                     RLSEMFNYLSLYNKRYIVAVTAGVALANIASPLGLIDVAVGSVSTLLVLLINYKITSR
                     IKNMKIKMAVTACVFAFSMFTVAGQLTILYQLPFFLNWWVIALGELASMIVGGIIIYW
                     IGKKVDLSK"
     gene            complement(5766..6305)
                     /locus_tag="ACG8KR_000940"
     CDS             complement(5766..6305)
                     /locus_tag="ACG8KR_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HFQ0200332.1"
                     /translation="MNRTLKELILAAEFAAIISVLSQMTIPFGLVPLTGQTFAIGLTA
                     TFLGRKLGLISVGVYLLLGLIGLPVFSGMTGGIAVLFGPTGGYLIGFLLQTWITGWMI
                     ERTDAHYLYAIFANLMGSLAALICGTIWLKISGDLTFTTAFASGLLPFLLPEAIKATG
                     AAYIGILMKKRLNRFLHTN"
     gene            complement(6445..7056)
                     /gene="rpsD"
                     /locus_tag="ACG8KR_000941"
     CDS             complement(6445..7056)
                     /gene="rpsD"
                     /locus_tag="ACG8KR_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HFQ0200333.1"
                     /translation="MSRYTGPSWKISRRLGISLSGTGKELARRPYAPGQHGPNSRGKQ
                     SEYGMQLTEKQKLRHMYGMNERQFRNLFVKASKIKEGKHGVNFMILLEQRLDNVVYRL
                     GLATTRRQARQLVNHGHILVDGKRVDIPSYHVEVGQVISVREKSQNIVTVKEAVEATV
                     GRPAFVSFDAEKLEGSLTRLPERDELYPEIDEALVVEYYNQSL"
     gene            7318..8169
                     /locus_tag="ACG8KR_000942"
     CDS             7318..8169
                     /locus_tag="ACG8KR_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HFQ0200334.1"
                     /translation="MRGYVQMEKSEGSLRRKSMNPVSPLFEKIDASIEKKANLFENSF
                     SRYAVRAVLACLFLTLGTAVAFAIAMKGEDIAHGLGKILYAFMFSWGLVMILYMNAEL
                     GTSNMLYMTVGVFRQRLALSKAAKILFACIFFNMIGGILCGFLISMTTPFQDLTLDNF
                     MLESIAVKLAKPSATILVEGMFANIVVNTAVLISMRMKEDVGKVITVVFIIFIFAFLG
                     YEHVIANFPAFTLTYFASHGQMDGMTVSNVLHNLFFALAGNYIGGGLVMGLGYAWLNQ
                     SKSSYVD"
     gene            8197..8832
                     /locus_tag="ACG8KR_000943"
     CDS             8197..8832
                     /locus_tag="ACG8KR_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HFQ0200335.1"
                     /translation="MEVLVFPKNEEKALQMEKYMKNLFSFAGVPKPERALLQQPLLDE
                     SKNLSISEVLAFVHYYYEQIEREYQYVAIDLVQKNIKRLTFEDLVVLLPLVQKKEWWD
                     SIDSWRKVYSTWCKAYPEQREEVFQLFEKKEDFWLRRIAITLQLGYKQQTDTGLLIRA
                     IDEDRQTKEFFIQKAIGWALRDYSKTDADWVKRQLTTDLSKLAVREASKYL"
     gene            8852..9625
                     /locus_tag="ACG8KR_000944"
     CDS             8852..9625
                     /locus_tag="ACG8KR_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFQ0200336.1"
                     /translation="MRRKLFAFDIDGTLLDSNKQALDSTREALEKLRKAGHLVTIATG
                     RSRFHAQEIIRDLDFTNYILCNGAAGFLDHEQVYKNLLDRDQLDAFVQEAHHEQIDTA
                     FVSLDNIKRSSSNDVTVMEEAMHSFGAVLPELDQFFAEEQEIYQALAFFDQTYDGKFS
                     SYDKIRFVRWHENSVDVVPKNGSKAATILHLAEQVGIPHEDIISFGDGQNDREMLRMS
                     GIGIAMGNAVPEVQAEAKMVTDTNDQDGIWKALKELSVI"
     gene            10033..10830
                     /locus_tag="ACG8KR_000945"
     CDS             10033..10830
                     /locus_tag="ACG8KR_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HFQ0200337.1"
                     /translation="MRVLFIGDVVGSMGREMITEYLPRLKKKYRPQVTIVNGENAASG
                     RGITEKIYKKFLQDGVDVVTMGNHTWDNRGIFEFVNEAKKMVRPANFPEGTPGQGMVF
                     VKVNQVELAVINMQARSFMVDLDDPFRKMNELVEEARKRTPIIFVDFHGETTSEKQAM
                     GWFLDGKVSAVVGTHTHVQTNDARILPRGTAYLTDVGMTGPYDGILGMRREPVIEKFL
                     TALPKRFEVVEQGRSILSGCILELDDTTGHAKEIQLIQINDDRPFME"
     gene            10808..11221
                     /locus_tag="ACG8KR_000946"
     CDS             10808..11221
                     /locus_tag="ACG8KR_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HFQ0200338.1"
                     /translation="MIDRSWSRKRGSFLQQEPEIQKEVRKLTKLLRENETIIRYKELE
                     EKIQQNQYLAELREKIKQAQKDAVHFAHYDKPAAEKEAIKQADQFMQEFDQHPLVVAY
                     RKQLLEADDLLHHLTTMIQEEINGQIEEEKHASKN"
     gene            11205..13850
                     /gene="mutS"
                     /locus_tag="ACG8KR_000947"
     CDS             11205..13850
                     /gene="mutS"
                     /locus_tag="ACG8KR_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HFQ0200339.1"
                     /translation="MPQKTKNTPMMEQYLSIKAQYQDAFLFYRLGDFYELFYEDAIKA
                     SQLLELTLTSRNRNAEEPIPMCGVPHHAAQGYIDALIEKGYKVAICEQVEDPKTTKGM
                     VKREVIQLVTPGTVMDSKGLSAKDNNFLTAVVSQGNEFGFAYADLSTGELKTARLHDE
                     EAVLNEASALQTKELVLGSEITDTLREQLSGRLGLVFSQQNEMEENAEFSFLTSELVD
                     PLEKEATGKLLTYLSVTQKRSLAHIQKAVEYQPDHFLKMDYYSKFNLELSQSIRTGKK
                     HGTLLWLLDETKTAMGGRLLKQWLDRPLIQAKQIKARQEMVASLLDSYFERIDLQSAL
                     TKVYDLERLAGRVAFGNVNGRDLIQLKTSLEQVPMIRGLIEGIDQGQWQSLLSEMQPM
                     DHLVQLIDQAIQDDPPLQITEGNIIKDGFNEQLDMYRSAMRNGKKWLAELEAKERQET
                     GIKNLKIGFNRVFGYYIEITKANLGNAELEKYERKQTLANAERFITPELKELETQILE
                     AEEKSVDLEYQLFLAVREEVKKAIQPLQVLAKAISAVDVLQSFATISERYQYVRPELV
                     SDKHQLLIKDGRHPVVEKVLGHQEYIPNSVEMAEDEMILLITGPNMSGKSTYMRQLAL
                     TVLMAQMGCFVPAKQAVLPIFDRIFTRIGASDDLIAGQSTFMVEMMEANQALRYATPN
                     SLILFDELGRGTATYDGMALAQAIIEYIHRHVQAKTLFSTHYHELTVLEKELPQLKNV
                     HVGAVEKDGEVVFLHKMMDGPADKSYGIHVAKIAGLPSNLLERAADILHTLENGDNGH
                     HTVPAEVEEKTKTAKQEQIKPPAEKVNQDHIQDRVKEENQQLSLFSELSENETEVIES
                     LKQINLLEMTPMDALNTLYELQKQL"
     gene            13868..15976
                     /gene="mutL"
                     /locus_tag="ACG8KR_000948"
     CDS             13868..15976
                     /gene="mutL"
                     /locus_tag="ACG8KR_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032300 - mismatch repair complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HFQ0200340.1"
                     /translation="MAKIQELSERLANQIAAGEVVERPASVVKELVENAIDAGSTQID
                     ILVEEAGLRTIQVIDNGEGILADDVENAFKRHATSKIHNRDDLFRIRTLGFRGEALPS
                     IASVSEMTLETANQEEKQGTYLSLKGGEVIEHRPAPLRQGTKITVSNLFFNTPARLKY
                     VKTLHTELANIGDIVNRLALSHPSIAFRLVHDGNKMLATAGNGDLKQTIAGIYGLSTA
                     KKMLKIEGKDLDFELFGYVSLPEVTRASRNYLSTIINGRFIKNFSLNKAIVAGYGSKL
                     MVGRFPIAVLEIKMDPLLVDVNVHPTKQEVRLSKEKELTELISNAIQKALSQENLIPS
                     AADNLRFKKKVPDQPKTEQIEIDLSYAFEEEKPKKPSSLNFDRETGTFFVEELKNTFV
                     DRIVDKSVENVENYSGFSEENTTYPEKTAKTVDKFGENVDNFVEKTVEKSEKKEFPIV
                     DNMEKTNEEQADKSVEEDEKIYEEELTLHPEFDFTQGNDKTLEQAIKKLETEKPVQRF
                     PQLEYFGQMHGTYLFAQSSDGLYIIDQHAAQERIKYEYFREKIGEVSDDLQELLVPFV
                     LDYPNSDALKLKEQKHLLEDAGIYLEEFGQNSFIVRAHPTWYPNGEEESIIREMIDML
                     LTTGSVSVKKFREATAIMMSCKRSIKANHYLNEAQARVLLKDLARCENPFNCPHGRPV
                     LIHFTNSDMERMFKRIQDPH"
     gene            15998..16558
                     /locus_tag="ACG8KR_000949"
     CDS             15998..16558
                     /locus_tag="ACG8KR_000949"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase"
                     /protein_id="HFQ0200341.1"
                     /translation="MKIVLASQSPRRKELLAHIVSEFEIEPADIDETPFPDEQPVDYV
                     RRMAEEKARTVWEQDEKEDHLVIASDTTVVLNQEIMGKPEDLAEAEFMLKKLSGETHT
                     VYTAVVLKTKEREERILAEAHVTFYPLTDEEIKRYLETGDYADKAGAYGIQNAASVFV
                     KEISGDYYSIVGFPIGAVNQALKTFI"
     gene            complement(16643..18631)
                     /gene="treP"
                     /locus_tag="ACG8KR_000950"
     CDS             complement(16643..18631)
                     /gene="treP"
                     /locus_tag="ACG8KR_000950"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015574 - trehalose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015771 - trehalose transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HFQ0200342.1"
                     /translation="MAKYQADAEKLLHDIGGKENIAAVSHCATRMRFVLNDPGKADEK
                     AIEDIPSVKGMFTNAGQFQVIIGNDVSTFYNDFVAVSGVEGVSKEQGKAAAKQNLHPV
                     QRAIAVLAEIFTPLIPAIIVGGLILGFRNVLEGIQFESLGGTIVEHSKFWNGVNAFLW
                     LPGEAIFHFLPVGITWSIAKKMGTTQILGIVLGITLVSPQLLNAYSVASTAAADIPFW
                     DFGFAQVQMIGYQAQVIPAMLAGFMLAYLEIFFRKYIPQSISMIFVPLFSLLPTVLAA
                     HVILGPIGWTVGSWISNIVNTGLTSSISWLFSAVFGFLYAPLVITGLHHMTNAIDMQL
                     IADFGSTNLWPMIALSNIAQGSAVLAIIFLHRGNKKEEQISIPAMISCYLGVTEPAMF
                     GINLKYVYPFVAAMVGSGLAGMFANLMGVRANAIGVGGLPGILAIQAETWVPFIIAMI
                     IAVIIPFGLTIIFRRQGILNKIDPAVPENAADVQLQTANGATATPQSFEPVSATGTAV
                     ATKETLFAVAAGTIKEITEVNDPVFSQKMMGDGYAVEPSNGKVYAPVNGKVTSVFETK
                     HAIGILSNEGLEVLVHMGLDTVELKGVPFNVFVKEGYLVTPETLIAEMDLPEIEQAGK
                     KTDIIVALTNNEKVAGLSLDQSGLVRPGEAVGKAEVKS"
     gene            18854..19567
                     /gene="treR"
                     /locus_tag="ACG8KR_000951"
     CDS             18854..19567
                     /gene="treR"
                     /locus_tag="ACG8KR_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HFQ0200343.1"
                     /translation="MNKFNEIFLDLEQKILAKEYPPHSLLPSENQLIKIYGVSRETIR
                     KALNLLTTEGYIQKKQGKGSIVLDRNRFDFPISGLTSYKELQETQRIPSETIIHTLEE
                     TEVTKALNEITGWEIGAPVWHLIRERKINGEVVILDTDYLLKEIVPILTPAQAQGSIY
                     EYFENELSLTIAYAQKEITVEEVTEIMKTAMDLHDDTYAVVVRSLVHLEDTRCFEYSE
                     SVHRLDKFRFVEFARRRKV"
     gene            19644..20090
                     /gene="msrB"
                     /locus_tag="ACG8KR_000952"
     CDS             19644..20090
                     /gene="msrB"
                     /locus_tag="ACG8KR_000952"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HFQ0200344.1"
                     /translation="MDKPIRPEKEELKEKLSPMAYAVTQENATERPFSGKYDDFYEKG
                     IYVDIVSGEPLFSSAEKYDAGCGWPSFSKPITKRQVREKADFSHGMHRIEVRSKEADS
                     HLGHVFTDGPVDQGGLRYCINSASLKFIPYEKMDELGYGEFKSLVE"
     gene            20260..20862
                     /gene="ruvA"
                     /locus_tag="ACG8KR_000953"
     CDS             20260..20862
                     /gene="ruvA"
                     /locus_tag="ACG8KR_000953"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HFQ0200345.1"
                     /translation="MYEYLTGVVTFINPYYIVVETNGIGYQIALGNPYRYSSKLNQKI
                     KIYVHQVIREDAHLLYGFDSLEEKQLFLRLVSVSGIGPKSALAIMASEDHSGLIQAVE
                     SSDVTYLTKFPGVGKKTAQQMILDLKGKFGELSIDTPFSLFDEANTKDATALSEAMEA
                     LSALGYSDREIKRVEKQLKEVDNQTTDEYLRQALKLMMKK"
     gene            20875..21876
                     /gene="ruvB"
                     /locus_tag="ACG8KR_000954"
     CDS             20875..21876
                     /gene="ruvB"
                     /locus_tag="ACG8KR_000954"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HFQ0200346.1"
                     /translation="MTDEYLLSPESGENELSLEKSLRPQLLSQYIGQRKVKQELQIYI
                     EAARNREEPLDHVLLYGPPGLGKTTMAMVIANEMTVNIRTTSGPAIERPGDLVAILNE
                     LEAGDVLFIDEIHRLPRVVEEMLYSAMEDFYVDIMVGQGPTAHPVHFPLPPFTLIGAT
                     TRAGMLSAPLRDRFGIISHMEYYDEQDLQEIVLRSSDIFQTEIIEEGALEIARRSRGT
                     PRIANRLLKRVRDFAQVQGDGKIDRPIADQALTLLQVDQAGLDYVDQKLLRTMIELYG
                     GGPVGLSTLSVNIGEERETVEDMYEPYLIQKGFLKRTPRGRIATAYAYEHFGYDYHI"
     gene            21905..22483
                     /locus_tag="ACG8KR_000955"
     CDS             21905..22483
                     /locus_tag="ACG8KR_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFQ0200347.1"
                     /translation="MSDTTRTKQLIKNVLIDLCQTQSFRKISVQKIANEAKINRQTFY
                     YHFTDKYDLLRWVYCEDALHYLDPQCLSLDNWEEQALKMLKAIAEKKEFYANTVLSDP
                     EILQKEFSQLIQKSFIKIFEQMDEERQLSARDKLFYARFFSYGCSGVLVNWITNEYEE
                     TPMEIAMQLFRLAKDTELYSYRLYAKEELEEQ"
     gene            22535..23017
                     /locus_tag="ACG8KR_000956"
     CDS             22535..23017
                     /locus_tag="ACG8KR_000956"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HFQ0200348.1"
                     /translation="MTKILFVCLGNICRSPMAEGLLRKRIALEGREAEFFVDSAATST
                     YEVGKTPHPGTKKILNQEKVDMTNMVARQITPHDFETFDWIIGMDQENVEELKRRAPR
                     SAQGKIHLFLSSIPGKEKENVPDPYYTNNFDETYHLITEGLNHWWNIWIDENKITNNL
                     "
     gene            23134..23508
                     /locus_tag="ACG8KR_000957"
     CDS             23134..23508
                     /locus_tag="ACG8KR_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0200349.1"
                     /translation="MNEETVSPMKKLTKLVTHTSYQLFDSFVNDTEEHAKNVRLQGKF
                     ILELQKAATEHSLVILQVRANRLSSKYETISGWVATKTVEKDRVMIRLQNEEQQIRMV
                     PVEQIEKITILSPAGEQERLSK"
BASE COUNT         7821 a         4300 c         5130 g         6685 t
ORIGIN      
        1 aaaaaaactt ggtacgcaat cgctgtacca agttttttta tttagctttt gattaagcca
       61 taaataataa tttatataag aatactgcta cgacaccagc acagataggt gctacaactg
      121 gtacccatgc atatccccat tgagaagaac ctttatgttt caatggaaga agtgcgtgta
      181 caagacgtgg agcaaagtcg cgggctgggt ttagtccagg acctgtagga ccaccaagtg
      241 aagcaactaa tgtccataca aggaaaccaa gtgctaagtg agctgtacca agattatctt
      301 ggaagaatgg tgcttttgta atacctaggg caccaaagaa taaaacgaat gaaccaaaga
      361 attcattgac gaaaccattg aatttagaac caacgctgtt gattgttgag aatgtaccta
      421 acacatggtt tacgtcttct gtttggtcgt agtaaggttt gtaacaagct actacgatca
      481 attgtccaat cgctgcacca atcaattgtg caatgatata aggaacaact tcagcccaag
      541 ggaatagacc attagatgct aaagcaattg taaatgctgg gttgatgtgg ttaccgctga
      601 ttccaccaaa aatcattgct ggcatcataa ccccgaatcc atacccgaca gcaatcagca
      661 tccagtcact gccatatcct tttgtacctt ttaaatctac gtttgctaca gcaccgttac
      721 ctagaacgat tagtaatgct gtaccaatca tttccgccgc aagacgggtc attagtgagt
      781 aatccataaa tacctccaaa agttataaaa atgcaaaaaa aaaagaaaca tcaacacgaa
      841 aagcatcggt gcagtgtttc gtgtcggaag gatttctgca gtcttaacaa atgtgaataa
      901 aaaaatgaat tccattactt ataagaaacc agaccaacag taaaactcca actcttttta
      961 aaagttctcc acaaatcacc aagcttaact ataaatatac caaaaaaaaa acgttttttt
     1021 tctcaaaaat tgtatcttag aaacagcatg ttttgaaact ttcagaaaag gtttgttagt
     1081 aaaatgaaag ctatacgtaa ggagtgttgt ttgatgaaat tttttcaaga gtttttcatt
     1141 attattttct tttctgtatt aggagaaggc atacggattt ttgctcattt acctattcct
     1201 ggaagtatct taggtattct ttttttattt ttagcatttg aaatgcatct tgttgatcct
     1261 gaaaaaatcg gagcaactgg tgatttttta ttgaataatc tagccatttt atttgttcct
     1321 gctggtgtag gtttattgga atattttgat gatattgctt cgatatggcc tgttttactg
     1381 ggagcagtcg tcgtgtgttc catcgtcacg atggttgcag cgggaaaaac ggcagaaggt
     1441 gtagaagctt tgctgggcta tgtccgtaag aaaaaagcag aaagggtatc tgtaaacgaa
     1501 atggaggaga atcaaattga ctgagttttt tgtgaatcca ttatttggct taagtttgac
     1561 tttaggagtg tacgtattat ctgacaaatt tttgcggaaa tataaactgc ctgttttgaa
     1621 tcctctcgta ttatcgattg ttgttattat cttatttttg catttttctg ggatcagcta
     1681 taaagactat aatatcgggg gcaatttttt gactatgctg attactcctg cgacagtggc
     1741 acttgccatc cctctttata aaaacttccg tttactgaaa gagaattttt atcctgtaat
     1801 ggctgcgatt ttagcaggaa tagtcgcaaa ttgcttggtt agtgtagggt tcggttactt
     1861 cttctctctt cataaagaga tggtgatctc attactacct aaatctgtaa cgacggctat
     1921 ttctgtcgat ttatctcata cgatgggcgg ggtgaatgcc gtgacacttg ctattgttgt
     1981 ctctacaggt atttttggtt cgctgatcgc cactcatatt ttccgcctat tcaagatcga
     2041 aagtccagtt gctcgcggtg tggcgttagg aagtacttct catgctattg gtacagcaaa
     2101 agcaatcgag attggtgaaa tagaaggaat catttccgga cttgcgatat gcgtcaacgg
     2161 aatattgacc gttttattgc taccgttatt ctttcagcct tttgctggct tgttttgatg
     2221 cgaaaaccac tattttatgc tacaataacc aaaacgttcg agcataaaaa aggagggaaa
     2281 gacgtgtatt tgtcttccga gtatcttaaa caatttgata aagatctata ttataagatt
     2341 ctcctcctag aaagttttga ggatcaagct tggcataccg ctgcacaatt agcgcaagtt
     2401 gttcagctgg atgcccgcag cgtgtcgaaa tacctgaacg agctgtctaa aaactatcag
     2461 cagtttagcg gtaagacaca ccctttgttt acaaaaaatc accgttctgg ttataacttt
     2521 tatgatacgc tcgattcaat tgaacatgag cgttttttga tctatctcgt gcaaagtacg
     2581 ctgaaattcc agttgctgca cgacattttt tttgaagaat tccatacaat gtaccaattt
     2641 gcgcaaaagc attacattag tgaatctact gctcatcgaa aaatcaatga atggaaacag
     2701 caactgcaaa cttacggaat tcggctgcaa cgaggtacat atatcgctca aggcgaagaa
     2761 gaaatcattc gactttactt gcatatgaca ttctggcagt tgtttcgagg gaaaatctgg
     2821 ccatttgaaa cgatttctca aatggacgta aagaatatgg cagaacatat catggctttt
     2881 tttaatgttc gtctgaacga gatcaaaaaa agacgattgg aatatatgct aggtgccttt
     2941 tttttaagaa aatcacagaa gcattatgtt gtattgaatg agaaaaaaag acggctgatt
     3001 tctgacaatc tgttgttcca gcgattttgc caggtcatgg agccggtatt tcctaattat
     3061 tttcaagtag aagacgagtt aggcgcatta tttcttgtac tgatgactcg tgaagaatat
     3121 tatagtgatc ctaagatacg gaagaaaatt tttgattttc atcaacaagc aaaaacaccg
     3181 ccctttacag ccctttcaga agcaaaagcc gctctttctc tttatcagga agagcagggg
     3241 ctgccagcag aaaatctaac cttcgaggca gaaaactatt tattcagcag tcattttttc
     3301 gcctatcttt ttcctaatgc gaaagaaacg attgatggca atagtagtga ttttattaat
     3361 catttagtta ttgaaaacaa ggaattgaaa caatggctcg tacatttttt tgaaagtaga
     3421 cacaaacatc ccaatcatct ggcatttaaa aatcatgcgt ttttgatggg gcggtatctg
     3481 actgtgttta aaacattagg agcctttact ccacagcttc caaagatcac gatcctttta
     3541 atgacagatt tcccattatt tgaagagcaa ctgttagagg agggattacg gacatttttc
     3601 cgtaatgagt atcagctgat ttttctgcca acagattatc gggggagaga agttgatctt
     3661 ctgatttcga caagtaaagt ccaccgcaaa ccatgggcgg atcttgacta ttttattgtg
     3721 actgaagaac taaaattaat tgattacatc caactatctc aaaaatttga aatgattcaa
     3781 aaacaaaaac tattaaaaca ataacaaaga aggagaaaaa agatgacatt tgaagaaatt
     3841 ttaccagaat tgaaaaaagg tgcaaagatt attcgtaaag gatggagtgg atttgaactt
     3901 tatgttgttc tcgtatccga tgatgaattt gatggctgtc cggtgacgcc gtacttattg
     3961 atcaaaacaa gtgacgaagg tttttccagc ttcgctccta cagtttgtga tattttagca
     4021 gatgattggg cgatcgtaga atgaaaaacc agtatccaga tctagctaaa caggttgtat
     4081 ttatcacagg agctgcttct ggaattggtg ctgcgcaagc ccgagcgttt cttgaacaag
     4141 atgcttttgt atttggatta gatcaaaagt tcggtaaaat ggaagaaatt tctttgcgat
     4201 ttcctgaaac ttttgcctac gcactaggtg atgttcgaca aatgaaagaa ctccaacaag
     4261 cggtatcgaa atgccaaaat cattttggtg aagttacgat tttactgaat acagcgggga
     4321 tattggatgc ctacaaaccc ttgatggaaa ctgacgaatc gctgtgggat ctgatttatg
     4381 agacgaatgt gaaaagcatg tatcagctga caaaaatcat ccttcctgat atgatccgcc
     4441 aaaaaagcgg aacgatcatc aatatggctt cgattgctgg cttgattgct ggtggaggtg
     4501 ggatcgctta tacaagcgcg aaacatgcga ttgtcggatt taccaaacaa ctcgctctgg
     4561 attacgcagg acagggtatc cgagtaaaag gaattgctcc aggagccatc caaacaccaa
     4621 tgaatgcggc tgattttgaa gggaaaggtg agatggcaga gtgggtggca aatgaaacac
     4681 cagtcaagcg atgggcacag ccggaagaag tagctgagct gacgttattt cttgcctctc
     4741 ctcaagcaag ctacatacaa ggaacgatca tcccaatcga cggcggatgg ttgttaaaat
     4801 aaccttgtgt ttttttcaag cttctgtatg ataaaactaa agagagaaac aacggttgat
     4861 gataaaaaaa gtcgatgact gttcccgaca ttcgatgccg aacagccagt tcaaaaaatt
     4921 ctttttaata atcgttaata ttcgaaacta agcggctttt cacgacctct tgatataaac
     4981 gatattcaaa agcttgcaac tgcggtaaca agtccagcca aatgaaaaat ataaggagct
     5041 atttttattt gaccattctt attacttgtt gcagggcagc tttttcacta ccttctgcga
     5101 cacttcagtt ggaattttct tcccatcctt tggtcgcggt cattaccaag ataaaattat
     5161 cttgcaaagg agaaatagaa catggaaaaa aataataccc gttcagccaa acgctggaca
     5221 acgacagatg tgactcggat ggcgctagtc actggtttat atgtggcagt gacgctggtt
     5281 ttgtctgtct ttagctttgg agtgattcag attcgtctct cggagatgtt caactatctg
     5341 tccttgtata acaaaagata catcgtcgcg gtaacagcgg gagtagcttt ggctaatatt
     5401 gcttctcctc ttggactgat agatgttgca gtaggaagcg tcagcacgct gcttgtattg
     5461 cttatcaact ataaaatcac tagtcgcatc aaaaacatga aaatcaaaat ggccgtgaca
     5521 gcatgcgtat ttgctttttc catgtttaca gtagctggtc agctaacgat tttatatcaa
     5581 ctaccttttt tcttgaattg gtgggtgatt gctttaggag aattggcatc aatgattgtc
     5641 ggtgggataa ttatttattg gatcggtaaa aaagtggatt tgtcaaaata aagatctgtt
     5701 taatttaaaa tagggcagca tggaagagac tgaatgttga gtcttcttcc atgctgcttt
     5761 tttgattaat ttgtatgaag aaatcgattt agccgttttt tcatcaagat accgatataa
     5821 gctgctccag ttgccttgat tgcttccggc agtaaaaacg gcagtagtcc actggcaaat
     5881 gcagttgtaa aagtaagatc accacttatt ttcagccaga tcgttccgca gatcaatgca
     5941 gcaagagagc ccattagatt ggcaaagatt gcatataagt aatgagcatc cgttctttcg
     6001 atcatccaac cagttatcca cgtttgtaag agaaatccaa tcaaatatcc gccagttggc
     6061 ccaaataaaa cagctatacc tccagtcatc cctgaaaata caggaagacc aatcaagcca
     6121 agtaaaagat aaactccaac agatatcaat cctagttttc ttccgagaaa agttgctgtt
     6181 aatccaatag caaatgtttg ccctgtcaaa ggaaccaaac caaaaggaat ggtcatctga
     6241 gaaaggacag aaataatcgc ggcaaattcc gcagcaagta tcaattcttt caatgttcgg
     6301 ttcataacgc cctcttttcg ttaacttaat atttttattt ggttaacgaa attgtaaaac
     6361 aaagtgatta ctttgtaaat agaaaatata aaaaaagacc agctatccaa tagatagcgg
     6421 tctttttcaa gaaagctttt gaaattataa gctttggttg tagtattcaa cgacaagagc
     6481 ttcgtcgatt tctggatata gttcgtcacg ttctggtaaa cgagtcaatg aaccttccaa
     6541 tttttcagcg tcaaagctta cgaatgctgg gcgtccaaca gttgcttcta cagcttcttt
     6601 tactgttacg atgttttgtg atttttcacg aactgagatc acttgaccca cttctacgtg
     6661 gtatgaaggg atatcaacgc gtttgccatc aactaaaatg tgaccatggt taactaattg
     6721 acgtgcttga cgacgagtcg ttgcaagtcc taaacggtaa acaacattgt ctaaacgttg
     6781 ttctagtaag atcatgaagt taacaccatg tttaccttct ttgattttgc tagcttttac
     6841 gaacaagtta cggaattgac gttcgttcat tccgtacatg tgacgaagtt tttgtttttc
     6901 agttaattgc atgccgtatt ctgattgttt gccacggcta ttaggtccat gttgacctgg
     6961 tgcgtaaggg cgacgtgcta attcttttcc tgtgcctgac aatgagatgc ctaagcgacg
     7021 agaaattttc catgatggtc ctgtataacg agacattaaa atttcctcca atgaatataa
     7081 gtttttggag taaaataatc cgttgaaaaa ttcataatca tatagtttgt tcttcaatct
     7141 tcacctttgc agccgcggtt acacaatcga acccgagacg gggcaacaaa ctagggatga
     7201 gttattattt ttctgctgca ttattttaca caaattacag tatatgatag atcatccacg
     7261 aatgtcaaga taattagtac ataaaaacgc tttacaaacc taggctgtct ttataaaatg
     7321 agagggtatg tgcaaatgga aaaaagcgaa ggaagtttaa ggagaaaatc aatgaatccg
     7381 gtatcaccat tatttgagaa aatcgatgct tcaattgaaa agaaggcgaa tctttttgaa
     7441 aacagctttt cacgttacgc tgttcgagcg gttctcgcct gtttgttttt gactttagga
     7501 acggctgtgg catttgcgat cgcgatgaag ggggaagata tcgcgcatgg tctaggaaag
     7561 attttatacg catttatgtt cagctggggt ctggtcatga ttctatacat gaacgccgaa
     7621 ttaggaacat cgaatatgct ttatatgacc gtgggtgttt ttcggcagag acttgcttta
     7681 agtaaggcag ccaagattct atttgcctgt atttttttca atatgattgg cggtatatta
     7741 tgtggatttc taatttctat gactacgcca tttcaagatt taacgttgga caactttatg
     7801 ttagaatcaa tagcagtaaa actagcaaaa ccttcagcaa cgattttggt ggaagggatg
     7861 tttgcgaata tcgttgtcaa taccgctgta ctgatcagca tgcggatgaa agaggatgtc
     7921 ggaaaagtga tcacagtcgt attcatcatt ttcatttttg cttttttagg atatgaacac
     7981 gtgatagcga attttcctgc ctttacgctt acttactttg cttctcacgg acagatggat
     8041 ggaatgacag tcagcaatgt gttacataat ttgttttttg ctttggcagg aaattatatt
     8101 ggtggcggtt tagtaatggg actgggatat gcttggctga atcagtcaaa atcatcctat
     8161 gtggactaaa aagtcgttca ttaaagggga agtgccatgg aagttttagt ttttccaaaa
     8221 aatgaggaaa aagcgttaca aatggaaaag tacatgaaaa atcttttttc ctttgctgga
     8281 gtacctaagc cggaacgcgc tcttttacaa caaccgttac tggatgagag caaaaattta
     8341 tctatttctg aagttttagc ttttgttcat tattattatg aacaaatcga aagagagtat
     8401 cagtatgtag cgatcgattt agtccagaaa aacatcaaac ggcttacatt tgaagactta
     8461 gtcgtattgt tgccactagt tcagaaaaaa gaatggtggg acagcatcga ctcttggaga
     8521 aaagtataca gcacatggtg taaagcctac ccagagcagc gtgaagaagt ctttcaattg
     8581 tttgaaaaaa aggaagattt ttggctacgt cgaatagcaa tcacgttgca attaggttat
     8641 aaacagcaaa cagacacagg actattgatc cgagcaatag acgaagatcg tcaaacaaag
     8701 gaattcttta tccaaaaagc aattggttgg gcattacgcg attatagtaa gaccgatgcc
     8761 gattgggtaa aaagacagct gacaacagat ttgagtaagt tagctgttcg agaggcaagt
     8821 aagtatttat aagaaagcga gacgacaaga gatgagacgc aaattatttg cttttgatat
     8881 tgatggaacg ttgttagatt caaacaaaca agctttagat agcacaagag aagcattgga
     8941 aaaattacgc aaagccggtc atctggtcac gattgctaca gggcggagcc gatttcatgc
     9001 gcaagagatc attcgcgatc ttgattttac gaactacatt ttatgtaatg gagcggccgg
     9061 ttttttagac catgaacaag tatataagaa tttactagat cgcgaccagt tggatgcgtt
     9121 cgttcaagaa gcgcatcatg agcagatcga tacagctttt gtcagcttag acaatatcaa
     9181 gcgttcttct tccaatgatg taacggtgat ggaagaagcg atgcattctt tcggtgctgt
     9241 cttgcctgaa ttagatcagt tttttgcaga ggaacaagaa atataccaag cgttagcatt
     9301 tttcgatcaa acatatgatg ggaagttctc ttcatatgat aaaattcgat ttgtccgctg
     9361 gcatgagaac agtgtagatg tcgtacctaa aaatggttct aaagcagcga caattctgca
     9421 cttagcagaa caagtaggca tccctcatga agacatcatc agctttggcg atggacaaaa
     9481 cgaccgtgaa atgttgcgca tgtcaggaat tgggatagct atggggaatg ctgtgccaga
     9541 agtacaagca gaagctaaga tggtcactga tacaaacgat caggatggaa tttggaaggc
     9601 attgaaagag ttaagcgtga tttaaaagag gttgtgaaaa agctgttctg catcaagtaa
     9661 taagcccagc caaacaaaaa tagctcctcc tatttttcgt ttttctgggc ttattaccgc
     9721 agatgcaagc tttcgaacac cgtttaagga agaggtcgtg acagaagtgg tcagtttcaa
     9781 gaaataagaa gggatttacg aaaattgctt ctcaaatttt tgtaaaatta cggcttattt
     9841 ctgaagaagt agatgaaaca acgtttattc gggaaatggg actgtgacaa gatgtttgtc
     9901 atagtcccat ttttttattt tatttttcaa aactaagaaa taccaggaaa gagttccttt
     9961 tgtttgacct tttctttctt attgaaaaca actataatat agagtgatgt taaaagagaa
    10021 gaggaattta gtgtgcgggt attgtttatt ggtgatgtag taggttcaat gggacgagag
    10081 atgattactg aatatcttcc tagattgaag aagaagtacc gtccgcaagt aacgattgta
    10141 aatggtgaga atgccgcttc tggacgtgga atcacagaaa aaatctataa aaaatttttg
    10201 caagacggcg tagatgtcgt gacgatgggg aatcatacgt gggacaaccg cggaatcttt
    10261 gagtttgtaa atgaagctaa aaagatggtt cgtccggcaa attttccgga agggacgcct
    10321 ggacaaggga tggtctttgt caaagtgaac caagtcgaac tggcagtgat caacatgcag
    10381 gctcgttctt ttatggtgga cttggatgat ccttttcgta aaatgaatga gctggtagaa
    10441 gaagccagaa aacgtacgcc gatcattttt gtagattttc atggagaaac aacaagtgaa
    10501 aaacaagcaa tgggctggtt tttagatggg aaagtttcag cagtggtcgg tacacataca
    10561 cacgttcaga caaatgatgc gcgaattttg ccaagaggaa cagcgtatct gacagacgtt
    10621 gggatgacag gtccctatga tggtattttg ggtatgcgca gagaacctgt aatcgaaaag
    10681 tttctgactg cattaccaaa acgttttgaa gttgtggaac aaggccgatc gattttaagc
    10741 ggctgtatct tggaattgga tgatacgaca ggtcatgcaa aagaaatcca gctgattcag
    10801 atcaacgatg atcgaccgtt catggagtag aaagagaggc agttttttgc agcaggagcc
    10861 ggaaattcaa aaagaagtaa ggaaactgac aaaactttta agagagaatg aaacgatcat
    10921 tcgctacaaa gaacttgaag aaaagatcca gcaaaaccag tatttagctg agttgagaga
    10981 aaaaatcaaa caagcgcaaa aagatgctgt tcattttgcc cactatgaca aaccagcagc
    11041 agaaaaagaa gcaatcaaac aagcggatca attcatgcaa gaatttgatc agcatccgct
    11101 tgttgtagct tatagaaaac agctgttgga agcagatgat ctgcttcatc atttaacaac
    11161 tatgatacaa gaagaaatca atggacaaat agaggaggaa aaacatgcct caaaaaacta
    11221 aaaatactcc tatgatggaa caatatctaa gcatcaaggc ccaataccaa gatgcttttt
    11281 tgttctatcg tttaggagac ttttatgaac tattttacga agatgcgatc aaagcgtcac
    11341 agcttttgga attaacgttg acgagcagaa atcgaaatgc agaagaacct attcctatgt
    11401 gcggtgtacc tcaccatgca gcacaaggat atatcgatgc cttgattgaa aaaggctata
    11461 aagtagcgat ttgcgagcag gtagaagatc caaagacgac taaagggatg gtcaagcgcg
    11521 aagtgatcca gctagtcact cctggtacag tcatggacag caaaggattg tcggcaaaag
    11581 acaacaattt tttaacagct gtagttagtc aaggaaatga atttggcttt gcttatgccg
    11641 atttaagtac gggagaattg aagacggctc ggttgcatga tgaagaagct gttttaaatg
    11701 aagcctctgc attgcagaca aaagaactcg tattaggcag tgagatcact gatacccttc
    11761 gtgaacagct aagcgggcgt ttagggctag tcttttctca acaaaatgag atggaagaaa
    11821 acgcggaatt tagctttttg acaagcgaac tagtcgatcc gctggaaaaa gaagcaaccg
    11881 gcaaactatt gacttattta tcggtgacac aaaagcggag tttagctcat atccaaaaag
    11941 cagtagaata ccaaccagat cattttttga aaatggatta ttattcaaaa ttcaacctag
    12001 aattaagcca atctattcgg actgggaaaa agcacggcac tcttctatgg ttgttagatg
    12061 aaacaaaaac agcaatgggt ggccgtttac taaaacaatg gctagaccgt ccgcttatcc
    12121 aggcaaaaca aattaaagca agacaagaaa tggtcgcttc tttgctagac agctattttg
    12181 aacgtatcga tttgcagtca gcattaacga aagtctatga tctggaacgc ctagcaggtc
    12241 gagtagcttt cggtaatgtc aatgggcgag acttgatcca gctgaaaact tctttagaac
    12301 aagtgccaat gatcagaggg ttgattgaag gaatcgatca aggtcaatgg caaagtctgc
    12361 tttcagaaat gcagccgatg gaccatctcg tccaattgat cgaccaagct atccaagatg
    12421 atccgccgtt acaaataacg gaaggaaaca tcatcaaaga tggattcaat gagcagctcg
    12481 atatgtaccg ttctgctatg agaaacggaa agaaatggtt ggcagaactt gaagcaaaag
    12541 agcgtcaaga aacaggaatc aagaacttaa aaatcggatt taaccgcgtt ttcggttatt
    12601 atatagaaat tacaaaagca aatcttggaa atgcagaact ggagaagtat gaacgaaaac
    12661 aaactttagc aaatgcagag cgtttcatca caccagaact aaaagaattg gagacacaaa
    12721 tcctcgaagc agaagaaaaa tctgttgatt tagaatatca gttgttttta gctgtgagag
    12781 aagaagtcaa aaaggctatc cagccgctac aagtacttgc aaaagcaatc agtgcagtag
    12841 atgtgctaca aagttttgca acgatcagcg aacgctatca gtatgttcgg ccagaacttg
    12901 tcagtgacaa gcatcagctt ctgatcaaag acggacgaca tcctgtagta gaaaaagtat
    12961 taggacatca ggaatatatc cctaacagcg tagaaatggc agaagacgaa atgatcttgc
    13021 tgattacagg accgaatatg tctggtaaaa gtacgtatat gcgacagttg gcattaactg
    13081 ttctgatggc gcaaatgggc tgtttcgttc cggcaaaaca agcagtttta cctatttttg
    13141 atcggatttt tacccgaatt ggtgcaagtg acgacttaat tgctggacaa agtactttta
    13201 tggtagagat gatggaagcc aatcaagccc tgcgttatgc tacgccaaac agtttgattt
    13261 tgtttgacga actagggcga ggaacagcaa cctatgacgg aatggcgttg gcccaagcaa
    13321 tcatcgaata tatccatcgt catgtacaag caaaaacact cttttctact cattatcatg
    13381 aactgacggt acttgagaaa gaacttccgc agcttaaaaa tgtccatgtt ggagccgttg
    13441 aaaaagacgg agaagttgtc ttcttgcata aaatgatgga tggacctgcg gataaaagct
    13501 acggcataca tgtggcaaag atcgcaggat tgccttccaa tctactagaa cgagcagcag
    13561 atatcctgca tacgctagaa aatggcgata acggacacca tactgttcca gctgaagtag
    13621 aagagaaaac taaaacagcc aaacaggagc aaataaaacc acctgcagaa aaagtaaatc
    13681 aagaccatat acaagaccgt gtaaaagaag aaaaccaaca gctttcttta ttcagtgaat
    13741 tatccgaaaa tgaaacagaa gtcattgaat cactgaagca aataaattta ctagaaatga
    13801 cgccaatgga tgcactaaac acattatatg aattacaaaa acaactgtag gaagaaggcg
    13861 gatatcgatg gccaaaatcc aagaattatc tgaacgttta gctaatcaga tcgctgcggg
    13921 agaagtagtg gagcgaccag cttccgttgt gaaagaatta gtggaaaatg caattgacgc
    13981 cggcagtact cagattgata tcttagttga agaagcggga ctgcggacga tccaagtgat
    14041 tgataacgga gaaggcattt tggcagacga cgtagagaat gccttcaagc ggcatgcgac
    14101 aagtaagatc cataatcgcg acgatctttt tcgcatacga acgctgggct ttcgagggga
    14161 agctttgcca agtattgcct ctgtttctga aatgactctg gaaacggcga atcaggaaga
    14221 aaaacaaggc acctatctgt cgctgaaagg cggagaagtt attgagcatc gaccagcacc
    14281 actgcgtcaa ggaacgaaga ttaccgtcag caatctgttt ttcaatacgc cagcccgctt
    14341 gaaatacgtg aaaacactgc acacagaatt agcgaacatc ggggacatcg tcaatcgact
    14401 ggcattgagt catccatcga ttgcttttcg tttagtacat gatggaaaca aaatgctggc
    14461 aactgctgga aatggcgatc tgaaacaaac aattgcggga atctacggat tatctaccgc
    14521 aaaaaaaatg ctgaaaatcg aaggaaaaga tctagatttt gagttatttg gctatgtttc
    14581 attgcctgag gtgactcgtg ccagtaggaa ttacttatcg acgatcatca atggacgttt
    14641 tatcaagaac ttttccttga ataaagcaat cgttgcaggt tatgggtcaa aattaatggt
    14701 cggtcgtttc cctattgctg tattggaaat caaaatggac ccgcttttag tagatgtaaa
    14761 tgttcatccg acgaagcagg aagtccggct gtcgaaggaa aaagaactaa cagagctaat
    14821 tagtaacgcc attcaaaaag cattgtctca agaaaatctg attccaagtg cagcggataa
    14881 tctacggttt aagaaaaaag tacctgatca gcctaaaaca gaacaaatcg aaatcgattt
    14941 gtcctacgct tttgaagaag aaaaacctaa aaagccaagt agtttgaatt ttgatcgaga
    15001 gacaggtacc tttttcgtag aagaactaaa gaacacattt gttgacagaa ttgttgataa
    15061 gtctgtggaa aatgttgaaa actattctgg tttttctgaa gaaaacacca cttatccaga
    15121 aaaaacagcg aaaacggtgg ataagtttgg tgaaaatgtt gataactttg tagaaaaaac
    15181 agtggaaaaa tctgaaaaga aagaatttcc cattgtggat aacatggaga aaacaaatga
    15241 agaacaagca gataaatccg tagaagagga tgaaaagatt tacgaggaag aattgacact
    15301 ccatcctgaa tttgatttca ctcaaggaaa cgacaaaaca ttagaacaag ccatcaagaa
    15361 attagaaacg gaaaaaccag tacaacgttt tcctcagttg gaatattttg gacagatgca
    15421 cggaacgtat ctttttgccc aaagtagcga tgggttgtac atcattgatc agcatgctgc
    15481 ccaagagcgc attaagtatg aatatttccg ggaaaaaatt ggggaagtat ctgatgatct
    15541 gcaagaattg cttgttccat ttgtgttaga ctatccaaac agtgatgctt tgaaattaaa
    15601 agagcaaaaa cacttgctag aagatgcagg gatttattta gaagaatttg gtcaaaacag
    15661 ctttattgta cgggcccatc ctacttggta tcccaatgga gaagaagaat cgattattcg
    15721 ggagatgatc gatatgctgc tgacaactgg aagtgtgagt gtaaaaaaat tccgtgaagc
    15781 aacagcaatc atgatgagtt gcaaacgatc gattaaagcc aaccattatt tgaatgaagc
    15841 acaagcacgt gtgctgctga aggatctggc acgatgcgaa aatcctttca attgtcccca
    15901 tggccgtcca gtattgattc actttaccaa ttcggatatg gaaaggatgt tcaaacgaat
    15961 ccaagacccg cattaagtca aagagaagag ggaaaacatg aagattgtat tagcatccca
    16021 atcaccaaga agaaaagagt tattggctca tatcgtttca gaattcgaga ttgaacctgc
    16081 tgatatcgat gaaacgccat ttccagacga acagcccgtc gattatgttc gtagaatggc
    16141 tgaagaaaaa gcacgaactg tctgggaaca agatgaaaaa gaagaccatt tagtgattgc
    16201 cagtgacacg actgttgttc tcaatcaaga gatcatgggt aaaccagaag atctggctga
    16261 ggctgaattc atgctgaaaa aactaagcgg ggaaacacat actgtatata cagctgttgt
    16321 tttaaaaaca aaggaacgag aagaacggat actagcagaa gcacatgtca ctttttatcc
    16381 tttgacagac gaagagatca aacgctactt ggagacgggg gattatgcgg ataaggcagg
    16441 ggcatacggc atccaaaatg ctgcgagtgt attcgtcaaa gaaatttctg gagattatta
    16501 cagtatcgta ggatttccga ttggtgctgt caatcaggca ctgaaaacat tcatttaaac
    16561 aaaaaagcgg actatctgat tttcgattga aaatccatga tagcccgttt tttttattga
    16621 aaagattact tatctactct gactaagatt ttacttcagc tttaccgact gcttcgcccg
    16681 ggcgaacaag tcctgattgg tcaagagaaa gtcctgcaac tttttcatta ttggtcaaag
    16741 caacaataat gtcagttttc ttgccagctt gttctatttc tggcaaatcc atttcagcaa
    16801 tcagggtctc tggtgtgaca aggtatcctt ctttcacgaa tacattgaaa ggaacaccct
    16861 ttagttcaac agtatctaat cccatgtgaa caagtacttc cagcccctca ttagaaagaa
    16921 taccaatggc atgttttgtt tcaaagacac tcgtcacttt accgtttact ggtgcataga
    16981 ctttgccgtt tgacggttcg acagcataac cgtcacccat cattttttgt gagaaaactg
    17041 ggtcatttac ttcagttatt tccttgatgg tccctgcagc gactgcgaat aatgtttctt
    17101 ttgttgcaac tgccgtacca gttgctgaaa caggttcgaa agactgtggc gttgctgttg
    17161 cgccatttgc tgtttgaagt tgtacatcag cagcattttc aggaacagca ggatcaatct
    17221 tattcaaaat tccctgacgt ctaaagatga tcgtcaagcc aaatggaatg atgacagcaa
    17281 tgatcattgc gatgatgaaa ggtacccatg tttctgcttg gatagctagt attcctggca
    17341 aaccgcctac tccgatcgcg ttagctcgaa cacccatcaa gttagcaaac atgccagcta
    17401 atccagagcc gaccattgca gcgacgaaag gatagacgta tttcaaattg attccgaaca
    17461 tagcaggttc agttacgcct aagtaacaag aaatcattgc tggaatcgag atttgttctt
    17521 cttttttatt cccacgatgt aagaagatga tggctaagac tgcagagcct tgggcgatat
    17581 ttgataaagc aatcatcggc cataaattcg ttgatccgaa atctgcaatc agctgcatat
    17641 cgatggcatt ggtcatatgg tggagaccag taatcactaa aggagcatac aggaatccga
    17701 atacggcact aaacaaccag ctgatagaag acgtcaatcc agtatttacg atatttgaga
    17761 tccagcttcc gactgtccag ccgataggac ctaaaataac gtgtgcagct aaaacagttg
    17821 gtaacaatga aaacaatgga acaaaaatca tcgagatcga ctgagggata tatttgcgga
    17881 agaagatttc tagatacgct aacataaacc ctgccagcat ggctggaata acttgcgctt
    17941 gataaccaat catctggact tgagcaaaac caaaatccca aaatggaata tctgctgcgg
    18001 ctgtggaagc tacactataa gcatttaata attgcggaga aactaacgta attcctaaaa
    18061 cgattcctaa tatttgtgta gtacccatct tttttgcaat actccaagtg atacctactg
    18121 gaagaaaatg gaaaattgct tcaccgggta accacaaaaa ggcattgaca ccattccaaa
    18181 acttcgagtg ttcaacgatt gttccaccaa gactttcaaa ctggatacct tcaagtacat
    18241 ttctgaatcc aaggatcaat ccaccgacaa tgattgcagg aatcagtggt gtaaagattt
    18301 ctgctaaaac ggcaatagca cgttgaacgg gatgaaggtt ttgttttgca gctgcttttc
    18361 cttgttcttt ggaaacccct tcaactccag atactgcaac gaagtcgtta taaaaagtag
    18421 aaacatcgtt tcctatgatg acttggaact gtcctgcatt cgtgaacatc ccttttacgc
    18481 taggtatatc ttcgatcgct ttttcgtccg cttttcctgg atcattcagg acaaaacgca
    18541 tacgagtcgc acaatgacta actgcagcaa tattttcttt tccgccgata tcatgtaaca
    18601 gcttctcggc atctgcttga tattttgcca tatcaataaa cccctttctc ttaacttgtc
    18661 tatgcaagtt gtttcttcat ttactatact ttttttcgga gagaattgca agcgttttat
    18721 taaaaagtac gagagaaaat gatatcgttt taaaaagctt aaaataagct tttcttaaaa
    18781 ataggaacat tgaaatacaa aaagagctgt agtatagtga agaaatgatg gaagaacgag
    18841 tgagggaaaa aacatgaata agttcaatga aattttttta gatttggaac agaaaatttt
    18901 agcgaaagaa tatcctccgc attcgctttt acccagtgag aaccaattga tcaaaattta
    18961 tggtgtttca cgagaaacaa ttagaaaagc gctgaatctt ttgactacgg aaggttatat
    19021 tcaaaaaaaa caaggaaaag gttcgatcgt actggatcgg aatcgcttcg actttcctat
    19081 ttctggattg actagctata aagagctaca ggagacccaa cgtattccaa gtgaaacgat
    19141 catccatacg ttagaagaaa cagaagtgac aaaagcattg aacgagatca ctggctggga
    19201 gatcggtgcg cctgtttggc atttgatacg cgaaagaaaa atcaatggcg aagtagttat
    19261 attagacacc gattatctat taaaggaaat cgttcctatt ttaactccag cacaagcaca
    19321 aggatcgatc tatgaatatt ttgaaaatga gctttctttg acgattgctt acgcgcaaaa
    19381 ggagatcaca gtagaagaag taacggaaat catgaaaaca gcgatggatc ttcacgatga
    19441 cacttatgca gtagtagtca gaagcctcgt tcatctggaa gatacccgct gtttcgagta
    19501 ttctgagtcg gttcaccgat tagataaatt ccgtttcgtt gagtttgccc gacgccgaaa
    19561 agtgtaaata gttcaaaaga aagcaaattg ttggcatctg tccactttgg cgctatgata
    19621 ggatcacaaa gggggcattt ttaatggata aaccaataag accagaaaaa gaagaactga
    19681 aagaaaagtt atcacccatg gcttatgcag tcacacaaga aaatgcaaca gaacgaccat
    19741 ttagcgggaa atatgatgat ttttacgaaa aggggatcta tgtagatatc gtcagcggtg
    19801 aaccgttatt cagctctgca gaaaaatatg atgcaggttg tggctggcca tcattcagca
    19861 aaccaataac gaaaagacaa gtgcgcgaaa aagccgattt ttctcatggc atgcatcgaa
    19921 tagaagttcg aagcaaagaa gcagattctc atctcggaca tgtgtttacc gatggaccag
    19981 tagatcaagg tggtctacgt tactgcatca attctgcctc actgaaattc attccatatg
    20041 aaaagatgga tgaattaggt tatggggaat tcaaatcact tgtagaataa tggaaataca
    20101 caaagagcgg tacaatacgg ggatttactc gatcgtccgc tcttttttta ttcaagcgat
    20161 tgctatttga aagaaacgct ccctagactc cttttttcac aaggtgaaat gtgctacaat
    20221 aaagggaacg tatgtgtgca aaagaaagag gactagaata tgtatgaata tctgacaggt
    20281 gtggttacct ttatcaaccc ctattacata gtagtagaaa ccaatggtat cggctaccag
    20341 atcgcactag gaaatcctta ccgctacagt agcaaactaa atcaaaaaat aaaaatttat
    20401 gtccatcagg taatacgtga agatgcgcat cttctgtatg gatttgattc gctagaagaa
    20461 aagcaactgt ttttgcgatt agtaagtgtt tcaggaatcg gtccgaaaag tgcccttgca
    20521 atcatggcaa gcgaagacca ctccggtttg attcaagcag tagaatccag tgatgtcact
    20581 tatttgacga aatttccagg agtagggaaa aagacagcac aacaaatgat cttggatcta
    20641 aaaggaaaat tcggtgagct gagtatcgat acacctttta gtctatttga tgaagcaaac
    20701 acaaaagacg ctacagctct atctgaagca atggaagctc tctcggctct tggttacagt
    20761 gatcgagaaa tcaaacgtgt agaaaaacaa ttaaaagaag tagataatca gacaacggat
    20821 gagtatctac gccaagcact caagctaatg atgaaaaaat aaggaggaga ccaaatgaca
    20881 gatgaatact tgttatcacc agaatctggc gaaaatgaac tgagtttgga aaaatctctt
    20941 cgaccacagc tgctttctca atatatcgga cagagaaaag tcaaacaaga acttcagatt
    21001 tacatcgaag ctgccagaaa tcgggaagaa cctttagatc atgttctgtt gtacgggcct
    21061 cctggattag gaaaaacgac gatggccatg gtcattgcta atgagatgac cgtaaatatt
    21121 cgtacgacga gtggccctgc tatcgaacgt ccgggtgact tagtagcaat cttgaatgaa
    21181 ctggaagcag gggatgtcct gtttatcgat gagatccatc gattacctag agtggtagaa
    21241 gagatgcttt attcagctat ggaagatttc tatgtagata tcatggtagg acaagggcca
    21301 accgcacatc cggttcattt ccctcttcct ccctttactt tgattggcgc gacgacacgg
    21361 gcaggtatgc tttctgcacc tttacgagat cgatttggca tcatttctca tatggaatat
    21421 tatgacgaac aggatctgca ggagatcgtt ttacgttctt cggatatttt tcaaacagag
    21481 atcattgaag aaggggcatt ggaaattgct cgccgatcac gaggaacgcc tagaatcgct
    21541 aatcgcttgc tcaaacgggt tcgtgatttt gcgcaagtcc aaggagacgg gaaaatcgat
    21601 cgtcctatag cggatcaggc acttactttg ctacaggtcg atcaggctgg attggattat
    21661 gttgaccaga aattgttgcg aacaatgatc gagttatatg gcggcggacc agtagggtta
    21721 agtacgcttt cagtaaacat aggagaagag cgtgagacgg tagaagatat gtatgaaccg
    21781 tatctgatcc aaaaaggatt tttaaagcgt acacctagag ggcgaattgc tactgcttat
    21841 gcctatgaac attttggtta cgactatcat atatgatttt tgcttttaga ttggaggaaa
    21901 atgaatgagt gatacaacac gtacgaaaca attgatcaaa aacgtattga ttgatttgtg
    21961 ccaaacgcaa tcttttcgaa aaatcagcgt ccaaaaaatc gcaaacgaag caaaaatcaa
    22021 tcgacaaaca ttttattatc attttacaga taaatacgat ttgcttcgtt gggtctattg
    22081 tgaggatgca ttgcattatt tagatccaca atgtctttca cttgataatt gggaagagca
    22141 agcattgaaa atgctgaaag caattgctga gaagaaagaa ttttatgcaa atactgtgct
    22201 ttctgaccca gaaattttac aaaaagagtt ttctcaactg atccaaaaat catttatcaa
    22261 gatttttgaa caaatggatg aagaaagaca actttctgca agagacaaac tattttatgc
    22321 tcgttttttt tcttacggtt gcagtggtgt attagtcaat tggatcacga atgaatatga
    22381 agaaacaccg atggaaatcg ctatgcagct tttccgttta gccaaggata cggaattata
    22441 ttcttatcgt ctttatgcaa aagaagagtt ggaagaacag taatagaaaa attttttgtt
    22501 ggaaaagcaa taatcgctaa aaggagatgg ggaaatgaca aaaattttat ttgtttgttt
    22561 gggaaatatt tgccgttcac cgatggcaga aggcttgctg agaaaacgaa tagcattaga
    22621 aggaagggaa gcagaatttt ttgtggattc tgctgctaca agcacgtatg aagttggaaa
    22681 gacaccgcac cctggaacga aaaaaatatt aaaccaagaa aaagtagata tgacgaatat
    22741 ggttgctcgg caaatcacac cccacgattt tgaaactttt gattggatca ttggcatgga
    22801 tcaagaaaat gtagaagaac tgaaacgaag agcaccacgc tcagcgcagg ggaaaatcca
    22861 tttgttttta agtagcattc ctggaaaaga aaaagaaaat gttcccgacc catactatac
    22921 caataacttt gatgagacat atcacttgat cacagaaggc ctgaatcatt ggtggaatat
    22981 ttggattgat gaaaataaaa taacaaacaa tttgtaaagt taacgctttc tttttaaggc
    23041 gatgttagag catttccttg tttttttgta aaatgttagg taaaataaaa ttaattaagt
    23101 aacactttct tagctaaagg aggagtgcat tcgatgaacg aagagacagt atcgccaatg
    23161 aagaagctta cgaagttagt tactcatact tcgtatcaac tttttgattc ttttgtaaat
    23221 gacactgaag aacatgctaa gaatgtacga ttgcaaggaa agtttattct tgagctgcaa
    23281 aaagctgcta ctgaacatag tctagttatc ttacaagtaa gagcaaatcg tttgagtagc
    23341 aagtatgaaa cgatctcagg ctgggtggcg acaaaaacgg tcgaaaaaga ccgagtgatg
    23401 atccgcctac aaaacgaaga gcagcagatc cggatggttc ctgtagaaca aattgaaaaa
    23461 ataacaatcc tttcaccagc aggggaacag gaacggttat caaaataatt tataccagat
    23521 agaaaagcac gtatgagctt gtaccatcaa tcattactaa tcaaaaataa ccgaatgctt
    23581 taagctgata tcagtaaaac ggaacgtttt aggatgagta tcacagcatg acagcattgg
    23641 gttgattagt tgaatcgatt ggatggtaca agtttttttg ataaaaaaag gtcctgctag
    23701 ccggggagct agcaggataa ggagttaaaa atgaaaaagt gttgttaggg ttgtttgttg
    23761 gtatgcttat ataatagtca ctagttgtga atattttgtg acgaaagttt tcgaaagttg
    23821 ttaagttttt ttgcgaaaac tatttttata tcatatatat gtctaaaatc tatattataa
    23881 aagaaatgtt gtttgatcag cttctcttcg gtattagttc aaaacaaaac ggcaaa
//
LOCUS       DAZKLD010000029        23426 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.029, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000029 DAZKLD010000000
VERSION     DAZKLD010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23426)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23426)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23426
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.029"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(98..1825)
                     /gene="ptsP"
                     /locus_tag="ACG8KR_000958"
     CDS             complement(98..1825)
                     /gene="ptsP"
                     /locus_tag="ACG8KR_000958"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFQ0200350.1"
                     /translation="MVEMLKGIAASDGVAVAKAYLLVQPDLTFSKATVEDTAAEEARL
                     DAALAKSTEELQQIREKAAQSLGEAEAQVFDAHLMVLSDPEMVGQIKQNIKDNSVNAE
                     SALKEVTDMYIGMFEAMEDNAYMQERAADIRDVAKRILAHLLGVTLPNPSMINEEVVV
                     VAHDLTPSDTAQLDRNFVKAFVTDIGGRTSHSAIMARSLEIPAIVGTKEITAKVKEGD
                     ILAVNGIEGDVIIDPTDEQKAEFEKAGADYAAQKAEWEKLKNAETVTADGKHFELAAN
                     IGTPKDLVGVHNNGGEAVGLYRTEFLYMDSPDFPTEDDQYEAYKAVLEGMEGKPVVVR
                     TMDIGGDKELPYLQLPHEMNPFLGYRALRISLSEQGDEMFRTQMRALLRASVHGNLRI
                     MFPMVATLKEFRAAKAIFEEEKQKLISEGKEVSDTIQVGIMIEIPAAAVLADKFAKEV
                     DFFSVGTNDLIQYTMAADRMNERVSYLYQPYNPSILRLIKNVIDAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSILKTRSLMKRLDTAKMAELADRALKECDMMEEV
                     VELVHEYVK"
     gene            complement(1825..2091)
                     /locus_tag="ACG8KR_000959"
     CDS             complement(1825..2091)
                     /locus_tag="ACG8KR_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010773230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HFQ0200351.1"
                     /translation="MEKKEFHVVAETGIHARPATLLVQTASKFNSDVNLEYKGKSVNL
                     KSIMGVMSLGVGQGSDVTITAEGADEADAMAAIVETMKKEGLSE"
     gene            2570..4804
                     /locus_tag="ACG8KR_000960"
     CDS             2570..4804
                     /locus_tag="ACG8KR_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFQ0200352.1"
                     /translation="MLCQNCGKNEATIHLYATVNGQRTQLDYCQSCYQKIKNQQNGGL
                     TNMAQNDPFGFGSLDDLFRSMSRQMQQQGNYEQTPPTQFGGNNNFNGGQPPQGNTEGL
                     LGEYGINITEQARQGDIDPVIGRDDEIKRVIEILNRRTKNNPVLIGEPGVGKTAVVEG
                     LAQKIVDGDVPQKLMDKEVIRLDVVSLVQGTGIRGQFEERMQKLIEEIRQAENVILFI
                     DEVHEIVGAGSAGDGNMDAGNILKPALARGELQMVGATTLNEYRIIEKDAALERRMQP
                     VRVDEPTVEETISILKGLQKRYEDYHHVKYTDEAIEAAATLSNRYVQDRFLPDKAIDL
                     LDETGSKKNLTIQIVDPKTIEKKLQEAEEQKVLASREEDFEKAAYYRDQINKLQKMKE
                     RQLTEEETPVITEKDMEKIVEQRTGIPVGELKEKEQTQLKNLADDLKAHVIGQDNAVD
                     RVAKAIRRNRVGLNKQNRPIGSFLFVGPTGVGKTELAKQLAYELFGSQDSMIRFDMSE
                     YMEKHSVSKLIGSPPGYVGYEEAGQLTEKVRRNPYSLVLLDEVEKAHPDVLHMFLQIL
                     DDGRLTDAQGRTVSFKDTIIIMTSNAGTGKVEANVGFGAAREGVTRSVLNQLNNYFTP
                     EFLNRFDGIIEFSALSKENLMTIVTLMLDDVNQMLAAQQLHIEVPTNVKEKLVDLGYD
                     PSMGARPLRRTIQEQIEDGIAEFYLDHPSIHELKAKLDKDGKIIVTSKPERLAKESAE
                     ETAE"
     gene            4952..5272
                     /locus_tag="ACG8KR_000961"
     CDS             4952..5272
                     /locus_tag="ACG8KR_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HFQ0200353.1"
                     /translation="MKLVNVTNSHSRLVLNQLENTDAHLVKVYTAGNTTIVYTEAPEH
                     NEILLINDKRKIQPKEIEDAKNYFLDKIKDAVYHEDEIKVIELAGLVEISIPKHELGA
                     TLAV"
     gene            complement(5477..5701)
                     /locus_tag="ACG8KR_000962"
     CDS             complement(5477..5701)
                     /locus_tag="ACG8KR_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein"
                     /protein_id="HFQ0200354.1"
                     /translation="METEWQRRERKKQKKEEMLRLCRLLDVKDWDKNEQIAQKVRSII
                     HKEEKVKNKDTRRTENEKRKVQKKREKSKS"
     gene            complement(5899..7479)
                     /locus_tag="ACG8KR_000963"
     CDS             complement(5899..7479)
                     /locus_tag="ACG8KR_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HFQ0200355.1"
                     /translation="MNNPKLKQQVDNRRTFAIISHPDAGKTTITEQLLLFGGAIRQAG
                     TVKGKKTGNFAKSDWMEIEKQRGISVTSSVMQFDYQGKRVNILDTPGHEDFSEDTYRT
                     LMAVDSAVMVIDSAKGIEAQTKKLFQVVKKRGIPIFTFINKLDRDGREPLELLEELEE
                     LLDIESYPMNWPIGMGKGLEGLYDIYHNRVEFYRPENYQDERLVELDEDGLLPENHPL
                     TENSLYEQVLEEVELIKEAGDAFNQEKIARGDQTPVFFGSALTNFGVQTFLETFVELA
                     PAPYGHKTQDDQMVSPYEEEFSGFVFKIQANMNPAHRDRIAFVRICSGTFERGMDVWL
                     ERTNKKLKLSNVTQFMADSRENVEKAVAGDIIGVYDTGNYQIGDTLFEGKLKVAYEEL
                     PSFTPELFMKVTAKNVMKQKSFHKGIYQLVQEGAIQLYKTYLTEEYIIGAVGQLQFEV
                     FQYRMLNEYNAEVIMSPMGHKIARWIDPEDLDEKMSSSRNILARDRFDQPLFLFENQF
                     AERWFADKYPNVKLKSLM"
     gene            complement(7880..9256)
                     /locus_tag="ACG8KR_000964"
     CDS             complement(7880..9256)
                     /locus_tag="ACG8KR_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HFQ0200356.1"
                     /translation="MNADPESQSLLAQILLLIVLTLINAFLAASEIAVVSINKNRIEQ
                     KADEGDVKSQKLLKILQNPNNFLSTIQVGITLVNILSGASLANTLSERLAPVLGGGTA
                     AKNIANIIVLAILTYVSIVFGELYPKRIALNKSEEVAKFTSGLIRLIGVVAKPFVWLL
                     SASTSLLARITPMTFDDEDSKMTRDEMRYMLENEGVLNNEELEMLQGVFSLDTKVARE
                     VMVPRTDAFMIDINDTVEENVNEVLSENYSRIPVYNEDKDKVVGILHTKNLLKAAHKF
                     GFDNLDIKKIMQEPLFVPETVFIDDLLYEMKKTQNQMAILLDEYGGVVGLATLEDLLE
                     EIVGEIDDESDEVENLYEKIDEHEYIIQGRMLIDEFNEAFDSDLHMSDVDTMAGYLIT
                     ALGMIPDEGEKLSFDVDNITLVSEEMEGSRILKIRVIFHDPEETEAEPEEERRYFRKE
                     FEDDEPRR"
     gene            complement(9384..10472)
                     /locus_tag="ACG8KR_000965"
     CDS             complement(9384..10472)
                     /locus_tag="ACG8KR_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFQ0200357.1"
                     /translation="MFWSLELLIIAALIMISSEINFIFEPIGTFFSTLFAPVLIAGFL
                     YYLLNPIVKLLMKAKIKRIWAVAIVLLLLVAAIIWILLSVIPSLVQQISSLASNMPDF
                     IKQVESWLKEVAELPLFKEVDINKYFEQLDISYGTIIQQFLSGVSNSLGSIVGTIASA
                     TVVIITAPFILFYMLKDGEKLVPNIQQFFPEKRRNQIVELLGQLNKTLANYISGQAIE
                     CLFVGTFTFLGYMVIGVDYAFLFGVIAGLTNLIPYLGPYLGLAPAFLVTVFNDPVKAL
                     LCCVVVLIVQQLDGNIIYPNVIGKSLKIHPLTIIIVLLVAGNIAGLLGIFLGVPFYAI
                     CKTIISYIIKIVKEDKQNENKKKIATTS"
     gene            10644..11078
                     /locus_tag="ACG8KR_000966"
     CDS             10644..11078
                     /locus_tag="ACG8KR_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0200358.1"
                     /translation="MKVVQTKDTMSNIYLDAVRIRHQVFVVEQGVPLSREIDKDEAHC
                     IHFVLYSDKKEPQGTVRLLPLENGKMKLQRMAILSEYRHQGLGKILIEEAENFAKNQG
                     YNTILLGAQSTAETFYEKLGYTAYGDPFEDAGMPHIYMKKTL"
     gene            complement(11156..11689)
                     /locus_tag="ACG8KR_000967"
     CDS             complement(11156..11689)
                     /locus_tag="ACG8KR_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HFQ0200359.1"
                     /translation="MGVPKEGEFVTIQSYKHDGKLHRTWRDTMVLKTSEHSMIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNVIAMIREKGVSYYCNLASPFLLDDEALKYI
                     DYDLDIKVFPDGEKRLLDVDEYEMHSKMMNYPNDIDFILKENVKILVDWINNGDGPFS
                     EGYIDIWYNRYKQLSRK"
     gene            complement(11783..12961)
                     /gene="mutY"
                     /locus_tag="ACG8KR_000968"
     CDS             complement(11783..12961)
                     /gene="mutY"
                     /locus_tag="ACG8KR_000968"
                     /EC_number="3.2.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HFQ0200360.1"
                     /translation="MNKEWEKWTDKETKEFQDQFIQWYEQEKRNLPWRYNRDPYRIWI
                     SEIMLQQTRVDTVIDYFYRFMEWFPTIEELANAPEEKLLKAWEGLGYYSRARNIQAAA
                     KQIMSEFDGEMPQTPEEISSLKGIGPYTTGAIASIAFGLPEPAVDGNVMRVVSRLFCI
                     EADIAKASSRKIFDEAMRKIIDEKHPGEFNQAMMDLGSAICTPTSPKCETCPIQAFCL
                     ANKRGIQTSFPVKTKKAKPKDVYYISAALQNHSGAYYFEERDSQKLLANMWTFPMMEV
                     TQEEYERLKKEWETKPEIDLFDDLVAEEEQNLPFEKQELFVWQTRHLGEVTHIFSHLK
                     WHVLLFYGRATEGAEQEFTENKTSKWLKPDAFDSVVFPKVQMKLVDQLEKNRNNRNPF
                     "
     gene            complement(12954..13751)
                     /gene="recX"
                     /locus_tag="ACG8KR_000969"
     CDS             complement(12954..13751)
                     /gene="recX"
                     /locus_tag="ACG8KR_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HFQ0200361.1"
                     /translation="MLTVTRISKGKGPFYRVDLSEGEALQVSEDVLVRYRLLKGRELD
                     EETIQEIKKSSGADFGFHLAMNYLSYQLRSEKEIRTYLKEKEILQEDRNKIVARLKEL
                     DLVDDLVYGESYVRTNMRLSDKGPKKLAQQMQQKGLKPEIIEQALSQYTFEDQVQNAH
                     QTAEKAFGKNHGKSQKELLRKIQQTLMTKGFSQEVIQQAIADLPQEADQEIEYDYLVK
                     QGDKLWRRNLRFEPKKRNLKVKQSLYQKGFDLDMIQRFITEKEEESE"
     gene            13858..15240
                     /gene="rlmD"
                     /locus_tag="ACG8KR_000970"
     CDS             13858..15240
                     /gene="rlmD"
                     /locus_tag="ACG8KR_000970"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HFQ0200362.1"
                     /translation="MAETIVKTGQTVPLKIKRLGINGEGIGYFKRLIIFVPYALPKEE
                     VLVKITKATPRYAEGELIKVKKTSKDRVVAPCPVYYECGGCQLQHLAYQAQLDFKKDL
                     LLQALEKFKPAGFRSYQLLPTIGMDEPWHYRNKAQFQLRKNKQTQKIEAGLYEANSHQ
                     LVPITDCLVQEPRTQKVMNTVVQLLNKFDAPIYDERTNSGIFRTIMVRIGVKTGELQV
                     VFITRSKKFPQKNAMIREINERLPEVVSIMQNVQAQKTSLVMGDETIHLWGKEAIEEQ
                     INEVTFDLSPRAFFQLNPQQTELLYGEGIKALDVQPNETIVDAYCGVGTIGLSVAKQA
                     KEVRGMDTVPQAIADAKKNAERLGYHNTRYEVGTAEQVLPKWLNEGFQPDGIIVDPPR
                     TGLDNALIQSLLKYPPKKLVYISCNVSTLARDLVNLSKKFKIEYLQSVDMFPQTARCE
                     VVVKMTRLAD"
     gene            complement(15414..15992)
                     /locus_tag="ACG8KR_000971"
     CDS             complement(15414..15992)
                     /locus_tag="ACG8KR_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic polysaccharide monooxygenase"
                     /protein_id="HFQ0200363.1"
                     /translation="MKKISTLLFIGLVSLGLFGFSIPGFAHGYITSPGSRAYLGTSAA
                     GNLNQNVGRAQWEPQSIEATKNTFIDGKIASAGVSGFEPLDEQTSNRWHKNMVNPGTL
                     NITWNLTAQHRTSTWDYYITKPTWNPNQPLKFSDFELITKIDDKATVPPKTVNQTITL
                     PQDRKGYNVILAVWNISDTTNAFYQVIDVNIQ"
     gene            16314..16784
                     /locus_tag="ACG8KR_000972"
     CDS             16314..16784
                     /locus_tag="ACG8KR_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TspO/MBR family protein"
                     /protein_id="HFQ0200364.1"
                     /translation="MKMIKDIRFWVCVIGIVILGFLSGLLSGNPGKYYYSLQLPPFAP
                     PSWIFGPMWTLLYILMGISLYLLLNHNNKKQRNNLVGLFVIQFIFNFIWSALFFNLRN
                     IFIAAIDITLLVIFLSVLMYQLWLHHRLAMWLMIPYYLWVLFATLLNYSIYFLN"
     gene            complement(16892..18118)
                     /locus_tag="ACG8KR_000973"
     CDS             complement(16892..18118)
                     /locus_tag="ACG8KR_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFQ0200365.1"
                     /translation="MKRWQKVLLTLLGVFVLLIAGLSAYGIRFMGEASQTVNKISKNS
                     NRISSKRSERVSIDDKEPFSVLLLGLDTGGLGRTEQGRSDTMMVVTVNPKQKKSTIIS
                     LDRDIYTNIVGYGTVDKLNHAYAFGGVEMAMDSIEQLLDIPIDHYVTINLDGMEDLIN
                     AVGGIKVNNKIDFTLDGVHVPVGEQTLNGEKGLAYSRMRHEDPEGDIGRQRRQREVVT
                     KIVNKVLSLDGVSNYRKILKAVENNVTTDLDWDDMLDVATNYTPAFETIKQDQLQGEG
                     QMINDIYYQILGKNDLLSIQNELKKQLNIKTSDTLPNLKNENASMMFFDDSEDGDSTN
                     GDGSNSEGYTDYSSYSENTYDQYQDPNTYYQQTEDTSQYYTPSQDTQSYDPYAGQEQN
                     GYGYDQNNEYGTTSGY"
     gene            complement(18872..19072)
                     /locus_tag="ACG8KR_000974"
     CDS             complement(18872..19072)
                     /locus_tag="ACG8KR_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003230776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFQ0200366.1"
                     /translation="MEHGTVKWFNNEKGFGFITVDGGDDVFVHFSAIQGDGFKSLEEG
                     QEVEFTIVEGARGPQAAEVTKL"
     gene            complement(19206..19814)
                     /locus_tag="ACG8KR_000975"
     CDS             complement(19206..19814)
                     /locus_tag="ACG8KR_000975"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HFQ0200367.1"
                     /translation="MTYTLPDLPYAYDALEPYIDEETMHLHHDKHHNTYVTNLNSAIE
                     KYPELGEKTIEELLSDMDAIPTDIKTAVRNNGGGHANHSFFWEIMAPNAGGEPTGEIK
                     EAINEAFGDFSSFKEEFKKAAAGRFGSGWAWLVMENGKLAITSTANQDSPLMEGKTPI
                     LGLDVWEHAYYLKYKNVRPDYIAAFWNVINWDEVNKRFEAAK"
     gene            complement(19899..20369)
                     /locus_tag="ACG8KR_000976"
     CDS             complement(19899..20369)
                     /locus_tag="ACG8KR_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HFQ0200368.1"
                     /translation="MKQYRQLKGYLEAKGYWEILVYLFFGGLATIVNFVSFALARQYF
                     DLSMALSNSISWFCSVLFAFVTNKLWVFHSKSPNFTHALIECGKFFFYRILSYGLDMG
                     AMVLLINVMNSNEYVAKIITQIIVILANYIFSKLFIFKETEVFEEEVGQSEKDD"
     gene            complement(20417..21331)
                     /locus_tag="ACG8KR_000977"
     CDS             complement(20417..21331)
                     /locus_tag="ACG8KR_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFQ0200369.1"
                     /translation="MEEGIVPTQPQRSSKKLFGVVVLNAVITLSEFIGGIMSGSLSLV
                     SDAFHNLSDTLAIIFSYGAQKMARKEANKKRTYGYQRLEILSAFINSFILIILSLFLT
                     VEAFKRFTSPEKINSHLMLTVAVIGLLANLFSVLLLRQEADESLNIKSSYLHLLSDTL
                     SSVSVIIGAILIRFFDIYWIDPVITLIISIYILIEAIMVIKKAAAILIQSAPAIDYEK
                     MEQEIEAIDGVKDVHHVHIWQYSEKIIIFDGHIDFEDQLLSEIEKIYPRIISLLKLKY
                     GITHVTIQAETKAKDQKKLVFLNKDESY"
     gene            21477..22280
                     /locus_tag="ACG8KR_000978"
     CDS             21477..22280
                     /locus_tag="ACG8KR_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HFQ0200370.1"
                     /translation="MTTKQLIISSFIRFKDLKEARNVPFWKAIVYLLVLSIIMALPIS
                     FQVFQVLNNIQLDGQKIATRIPDFTIQNGQLQTEEKEGFIYQTNSIIFTFDPEGKRTE
                     QDISTDLMGNFVSVGMLKNKLIIALPNTGTTSALLNNNQLELPYTNESLKNLTGKQLR
                     SFLSEASIPLWIKVLTFIFSIYPSFLNLLITLLFTNIAAFLYARFRLTKATFLDCLKT
                     LIYSISLPTIIATILMVFLPSFDTSAFIAFAGIFIFAQAVKGWAKISIN"
     gene            22458..23405
                     /locus_tag="ACG8KR_000979"
     CDS             22458..23405
                     /locus_tag="ACG8KR_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HFQ0200371.1"
                     /translation="MSERIIYADRPFRDEFIEEIHTIAPGYIFKTELQPEDLQNVEIS
                     LGWNSDYQEKLLASSTLKWVQSISAGVDQLPLETFSKKHILLSNGSGIHSESITEHVL
                     GIILGYSRGLFQAQKAQSDMKWLGSSVHYQSIQGKKILIIGTGHIGKMIAKKCKALDM
                     NCYGINTSGHPAEGMEKTFSLNQLKEVLPEVDFVVNILPLTEETTGLFDQHMFEHFDP
                     KSVFINVGRGASVKTQDLVQALNNQQLSFAALDVFEEEPLPQDHPLWKMDNVLITSHI
                     AGLTPDFQKKLMAIFLTNLKSYFATHELQTNQVKLSAGY"
BASE COUNT         7094 a         4701 c         3955 g         7676 t
ORIGIN      
        1 tttttgtcta ttcttactga caaaaacgtt ttcttaaaaa agaacaaaaa aactggcgta
       61 gctcttttac aagccacacc agcttttgat acgttgttta ttttacatat tcatgaacaa
      121 gttcaacgac ttcttccatc atatcgcatt ctttcaatgc acgatcagca agttcagcca
      181 ttttcgctgt gtccaagcgt ttcattaaac tacgcgtttt aagaatagat gtcgcactca
      241 ttgagaactc atctagaccc atacctacta gcaatggaac agctgtttga tctccagcca
      301 tttctccaca catacctgcc cattttcctt cagcgtgtgc tgcatcaatt acatttttga
      361 tcaaacgtaa aattgatggg ttatatggtt ggtacaagta agaaacgcgt tcgttcatac
      421 ggtctgctgc cattgtgtat tgaatcaaat cgttcgtacc tacagagaag aaatctactt
      481 cttttgcaaa cttgtctgct aagactgctg ctgctggaat ctcgatcatg atgccgactt
      541 ggatcgtatc agatacttcc ttgccttcac ttatcaattt ttgtttttct tcttcaaaga
      601 tcgcttttgc tgcgcggaat tctttgagtg tagctaccat agggaacatg atacgtaaat
      661 taccatgaac agatgcacga agcaatgcac gcatttgtgt acggaacatt tcgtcgcctt
      721 gttcagataa actgatacgc aatgcacgat atcctaagaa tggattcatt tcatgaggta
      781 attgcagata tggtagttct ttatctccac cgatgtccat tgtacgaaca actacaggtt
      841 ttccttccat accttctaat acagctttgt aagcttcata ttggtcatct tctgttggga
      901 agtctggaga atccatatac aggaattcag tacggtacag accaactgct tctccaccat
      961 tattatggac accaactaga tcttttggtg ttccgatgtt tgcagctaat tcaaaatgct
     1021 tgccgtcagc tgtgacagtt tctgcatttt ttaatttttc ccattctgct ttttgtgctg
     1081 cgtaatctgc acctgctttt tcaaattcag ctttttgttc atctgtagga tcgatgatta
     1141 catcgccttc gatcccgttg acagccaaaa tatcgccttc tttgacttta gcagtaattt
     1201 cttttgtccc aacgattgct ggaatttcta aagaacgtgc catgatagca gaatgtgaag
     1261 tacgtccacc aatgtctgtt acaaacgctt ttacaaaatt gcgatctaat tgtgctgtat
     1321 cacttggagt caagtcatgt gcaacaacga ccacttcttc gttgatcatt gatggatttg
     1381 gtaaagtaac gcctaataag tgcgctaaaa tacgtttagc aacgtcacga atatctgcag
     1441 cacgctcttg catataagca ttatcttcca ttgcttcgaa cataccgata tacatatccg
     1501 ttacttcttt tagcgctgat tcagcattta cgctgttgtc tttaatattt tgcttgattt
     1561 gacctaccat ttctggatct gaaagaacca tcaaatgggc atcaaatact tgcgcttctg
     1621 cttcacctaa gctttgcgct gctttttcac gaatttgctg caattcttca gtagatttag
     1681 ctaatgcagc atccaaacga gcttcttcag ccgcagtatc ttctactgtt gccttactga
     1741 atgttaaatc cggttgaact agcaggtaag cttttgcaac ggctacaccg tcactagcgg
     1801 caatcccttt tagcatttca accattattc agataagcct tctttcttca ttgtttcaac
     1861 gattgctgcc attgcatcag cttcgtcagc accttctgca gtaatagtaa catcagaacc
     1921 ttgacctaca cctaaagaca taacgcccat gattgatttc aagtttactg atttaccttt
     1981 atactctaag tttacatcag agttaaattt gcttgctgtt tgtactaata gtgtagctgg
     2041 acgagcgtgg atcccagttt ctgctactac gtgaaattct ttcttttcca tattaatcgg
     2101 tctcctttaa gtgagtgttg tttttatgtg agggttttct tttttagtaa ttcagagaat
     2161 cctaacaaat gccacccttt catctgtaaa gattaacatt ttttttcgtt agatacaaca
     2221 gttttcattc aatttcagat aaaagtattc aaattattcc ggattctttt tcatcctttt
     2281 cttcacattt tataacaaac gtgtttattt cctttttctt gtcgctttct cttctcctga
     2341 cgtttatcat tctttccttt gaccctcttt gttttcatat gaaacgaatt acttgcttcc
     2401 ctttttattt ttagtataat aagagtcaaa ggtcaggaat ggtcaataga gatttcacct
     2461 tttctacctc ataaatctct taaaaaacta ggggttattc ttttacctca tgaaaaacga
     2521 gatgtaaaat atggatgtac ccatttttgt gaaaggacgt gaatgattca tgctttgtca
     2581 aaactgcggc aagaatgagg caacgattca cttgtatgcc actgtcaatg gtcagcggac
     2641 acaactcgat tactgtcaga gctgttatca aaaaatcaaa aatcaacaaa atggaggtct
     2701 gactaatatg gcccaaaatg atccatttgg atttggcagt ttggacgatc ttttccgttc
     2761 catgtctcgc caaatgcaac agcaaggaaa ttatgagcaa acaccaccaa cacaatttgg
     2821 cggaaacaac aacttcaatg gtggtcagcc acctcaaggc aatacagaag gtttattggg
     2881 agaatacgga atcaatatca cagaacaagc acgacaaggt gatatcgatc ctgtgatcgg
     2941 acgcgatgac gaaatcaaac gagtcattga aattttaaat cgccgaacca agaacaatcc
     3001 tgtcttgatt ggagaaccag gtgttggtaa aacagcagtt gttgaaggtt tagcacaaaa
     3061 aatcgtagat ggtgatgtac cgcaaaaatt aatggacaaa gaagtcattc gtttagatgt
     3121 cgtatcttta gtccaaggta ctggtatacg aggacaattt gaagaacgta tgcaaaaatt
     3181 gattgaagag atccgtcaag cagaaaatgt gatcctattc atagatgaag ttcacgaaat
     3241 cgtcggtgct ggttctgctg gtgatggaaa tatggatgct ggtaatattt taaaaccagc
     3301 tttagctcga ggagaattgc aaatggttgg tgctactacc ttaaatgagt accgcatcat
     3361 cgaaaaagac gctgctttag aacgtcgtat gcaacctgta cgggtagatg aaccaactgt
     3421 cgaagaaacg atcagtattt tgaaaggttt gcaaaaacgt tatgaagact atcatcatgt
     3481 aaaatatact gatgaggcca tcgaagcagc cgcaacttta tccaatcgtt acgtccaaga
     3541 tcgcttcttg ccggataaag ccatcgattt gctagatgaa acaggttcta agaaaaactt
     3601 gacgatccaa atcgttgatc ctaaaacaat cgagaaaaaa ctgcaagaag cagaagaaca
     3661 aaaagtttta gcttctcgag aagaagattt cgaaaaagca gcttattatc gcgatcagat
     3721 caataaactt caaaaaatga aagaacgtca gctcactgaa gaggaaacac ctgtgattac
     3781 cgaaaaagat atggaaaaaa tcgttgaaca acggacaggt attccagtgg gtgaattaaa
     3841 agaaaaagaa cagacacaac tgaaaaactt agcagatgac ttgaaagctc atgtcatcgg
     3901 acaagataac gctgtagatc gagtagcgaa agcgattcgc cgaaaccgtg ttggattaaa
     3961 caaacagaat cgtccaatcg gctccttctt atttgttgga ccaactggtg taggtaaaac
     4021 agagcttgcg aaacaacttg cctacgaact attcggttca caagattcaa tgattcgttt
     4081 cgatatgtct gaatatatgg aaaaacatag tgtctctaaa ttgattggtt ctcctccagg
     4141 ttatgtcgga tacgaagaag ctggtcaatt aacagaaaaa gtacggagaa atccttacag
     4201 tcttgtcttg ttagatgaag tagaaaaagc tcatccagat gttttgcata tgttcttgca
     4261 aatcttagat gatggtcgat tgacagatgc acaaggaaga acggtcagct ttaaagatac
     4321 gatcatcatt atgacaagta atgccggaac tggtaaagta gaagcaaacg ttggttttgg
     4381 tgcggcacgc gaaggtgtta ctcgttctgt attgaaccaa ttaaacaact actttacacc
     4441 agaatttctg aaccgttttg acgggatcat cgaattcagt gcattatcaa aagaaaactt
     4501 gatgacaatc gtcacactaa tgctagacga tgtcaaccaa atgctggcag cacagcaact
     4561 tcacatcgaa gttcctacga atgtcaaaga aaaattagtt gacttaggct atgatccatc
     4621 tatgggtgct cgtcctcttc gacgcacgat ccaagaacaa atcgaagatg gtatcgctga
     4681 attttatctt gatcaccctt ctatccatga gttgaaagca aaactggata aagatggaaa
     4741 aatcatcgtt acttctaaac cagaacgttt ggctaaagaa tcagccgaag aaacggctga
     4801 ataaatccag ttttatagcg tgagacggta tttctttgag aaatgccgtc tcattctttt
     4861 ttcagaaaag tcaaaatact gttcatttta taaaaaagaa attataataa gagaaacaag
     4921 caattaaaaa tacataaaag aggtgagcgc catgaaattg gtaaatgtaa caaacagcca
     4981 ttcccgtctt gtattaaacc agcttgaaaa cactgatgcc catctagtca aggtctacac
     5041 agccggaaat accactattg tctacactga agcccctgaa cataatgaaa ttctgctgat
     5101 caatgacaaa cgtaaaatcc agccgaaaga aatcgaagat gctaaaaatt atttcttaga
     5161 taaaatcaaa gacgccgttt accacgagga tgaaatcaaa gtaatcgaat tggcaggcct
     5221 tgttgaaatc tcgattccaa aacatgaatt aggcgctaca ttagccgtgt aaacaaattt
     5281 tgaacataat aattaaagag cgtgggacaa aagtaaagaa cacttttgtc tcacgctcta
     5341 aacacgtata aacggcgaaa gcagaagcaa ctccttcgga aataagctga aattcacgaa
     5401 aattgctttt caaatttttg tgaattccag cttatttctt ggagttaaac gcttctgtct
     5461 cagcctcttt ttctttctag ctttttgatt tttctctttt cttttgtact tttctctttt
     5521 cattttcagt tctacgcgtg tctttatttt taactttttc ctctttatgg atgattgatc
     5581 gtactttttg tgcaatttgc tcgtttttgt cccagtcttt tacatctaat aatcgacata
     5641 atcgtaacat ctcttctttt ttctgttttt ttctttcacg tctttgccat tctgtttcca
     5701 tgtgtcacac tctcctttct ttaatcatat gtacgcaaaa taatttttat atttttttga
     5761 ttagacattt ctatgatttt aatatagtaa aaagagccgt cacggaaaat ccgtcacggc
     5821 tctaaacaaa atatgagagg ctgtttttgt ttggctattc ttattacttg atgcagggca
     5881 gcttgtgtca agacttcttt acatcaaact ttttaatttt acattgggat atttatccgc
     5941 aaaccagcgt tctgcaaatt ggttctcgaa taagaacaac ggttggtcga aacggtcacg
     6001 agccaagata ttgcgacttg aactcatctt ctcatccaaa tcttctggat cgatccagcg
     6061 agcgattttg tgtcccattg gagacatgat tacttcggcg ttatactcat ttaacatacg
     6121 atattggaat acttcgaatt gtagctgacc aacagctcca atgatatatt cttccgttag
     6181 atatgtttta taaagctgaa tcgctccttc ttgaactaat tgatagattc ctttgtggaa
     6241 ggatttttgt ttcatgacat tttttgcagt cactttcata aacagctctg gagtaaatga
     6301 agggagttct tcataagcaa cttttagttt tccttcgaac aatgtatccc caatctgata
     6361 attgcctgta tcatatacac cgatgatatc gcctgctact gctttttcga cgttttctct
     6421 cgaatctgcc ataaattgtg tgacattgct taatttcagt tttttatttg tacgttccaa
     6481 ccagacatcc attcctcgtt cgaatgttcc agaacagatt cggacaaaag cgattcggtc
     6541 acgatgggct ggattcatat ttgcttggat cttgaatacg aaacctgaaa attcttcctc
     6601 ataaggactt accatctgat catcttgtgt tttgtgtcca taaggagctg gtgccaattc
     6661 aacgaaagtc tctaaaaaag tctgtacacc gaagttagtc aatgcagaac caaaaaatac
     6721 tggtgtctga tcgccacgag caattttttc ttgattaaat gcatctccag cctctttgat
     6781 tagttctact tcttctaaga cttgttcata taaggaattt tcagttagag gatgattttc
     6841 gggaagcagt ccatcttcat ccaattctac taatcgttca tcctgataat tttccggacg
     6901 ataaaactct acacgattat ggtagatatc ataaagacct tctagccctt tacccatacc
     6961 gattggccaa ttcatcggat aagattcgat atctaatagc tcttctaatt cttctaacaa
     7021 ttccaatggt tcacgaccat cacgatctag tttgttgata aaagtaaata tcgggatgcc
     7081 acgttttttt acaacttgga ataatttctt tgtctgtgct tcgatccctt ttgcactatc
     7141 gattaccatg accgcactat ccacggccat caaagtacga taagtatctt cagaaaaatc
     7201 ttcatgtcct ggtgtatcca agatatttac tcgtttccct tgataatcaa actgcataac
     7261 agaactagta acagaaatcc cacgttgttt ctcgatttcc atccagtctg atttggcaaa
     7321 attacctgtt tttttgcctt ttacggtccc ggcttgacgg atagccccac caaacaatag
     7381 taattgttct gtaatcgttg ttttcccagc atccggatgg gaaatgatcg caaaagttct
     7441 gcgattgtcc acttgttgct ttaattttgg attattcata acctaactcc atttcttcca
     7501 ttcaattatt tatattttct ctaggcacag attgtccgat ctcttaaaga aagacaattt
     7561 catatagggc tgtgacaaaa gccgtgtagc agtctctttt tccgagtaaa cggcgttcta
     7621 gagacagcct atcagcaata agcccaccca agccaaaaat acgaaaagct gtttttgctt
     7681 ggctgctctt attacttaat gcaggatagc tttttcacaa cctttgtcaa ctactacaac
     7741 tgaatcagta tagcatggat tcagcgaatg aaaaagccac caaaccataa gagtcaaata
     7801 tttttttgtc agctgactaa aagaaaagac gtgttgcttg tttattcaag caacacgtct
     7861 ttcataacca agtttccaat tatcttcttg gctcgtcatc ttcgaattct tttctgaagt
     7921 atcttcgttc ttcttcaggt tccgcttctg tttcttccgg atcatgaaaa atgacacgga
     7981 ttttaaggat tcgagaacct tccatttctt cagaaaccag cgtaatatta tcgacatcaa
     8041 acgataactt ttcgccttca tccggaatca tgcctaatgc tgtaatcaaa taaccagcca
     8101 tcgtatctac atcactcata tgcagatcac tgtcaaaagc ttcattgaac tcatcaatca
     8161 gcatacgtcc ttggatgata tattcgtgtt catcgatttt ttcataaaga ttctcaactt
     8221 catctgattc atcatcgatt tctccgacga tttcttctaa aaggtcttct agtgttgcta
     8281 acccaacgac gccgccatat tcatctaata aaatagccat ctgattctgc gtctttttca
     8341 tttcatataa cagatcatca ataaagaccg tttcaggaac gaataaaggc tcctgcatga
     8401 ttttcttgat atctaaatta tcaaagccga atttatgggc agcttttagt aaattctttg
     8461 tatgaaggat tccgactact ttatctttgt cttcgttata aacagggatt cttgaatagt
     8521 tttcggataa aacttcattc acattttctt ccacagtgtc gttgatatca atcataaatg
     8581 catccgttcg tgggaccatg acttctcggg caactttcgt atcaagagaa aaaacccctt
     8641 ggagcatttc taattcttca ttattcaata cgccttcgtt ttcaagcata taacgcattt
     8701 catctctggt catttttgaa tcttcatcat caaaagtcat cggcgtgata cgcgccaata
     8761 aacttgtgga agcagaaagc agccagacga agggcttagc tactacacca atcaagcgta
     8821 tcagccctga agtgaacttt gcgacttctt ctgatttatt taaagcaata cgtttagggt
     8881 acaattctcc aaaaacaatc gatacatagg tcaaaatagc cagcacaatg atatttgcaa
     8941 tattttttgc cgctgtgccg ccacctaata ccggagctaa ccgttccgat aaagtattag
     9001 ctagtgaagc accagacaaa atgttgacca aagtgattcc tacttggatt gtcgatagga
     9061 aattatttgg attctgcaat atttttaaaa gcttttgaga tttcacatct ccttcatcag
     9121 ctttttgctc gattcgattc ttattgatcg aaacaacagc aatttcggaa gctgctaaaa
     9181 aagcattgat caatgtcaaa acgatcaata ataaaatctg cgctaacagc gactgactct
     9241 cagggtcagc attcataatc gcattctctc cttataaaat taatttttag tttacgatag
     9301 aaaaaaacgt ggcactatag ccacgttaaa atggtatcac aaagcaattt tctttgcaat
     9361 aaaaacaagt gtactaaaac tacctacgag gtcgtagcaa ttttcttctt gttttcattt
     9421 tgcttgtctt ctttgacgat tttgattata taggaaatga tcgtcttgca gattgcgtaa
     9481 aaaggaacac ctaagaagat accaagcaat ccggcaatat ttccagctac tagaagaacg
     9541 ataatgatcg ttaatggatg gattttcagt gatttcccaa ttacattagg ataaatgata
     9601 ttcccatcca actgttggac gattagcaca accacacaac aaagtaatgc ttttaccgga
     9661 tcattgaaga ctgttactag aaatgcaggt gctagtccta gataaggtcc taaatatgga
     9721 attaggtttg tcaatcccgc aatgacccca aataagaaag cataatccac accaattacc
     9781 atatatccta aaaatgtgaa ggtaccaacg aataaacatt cgattgcttg tccgctaatg
     9841 taattagcca atgttttatt cagttgcccc aataactcca caatttggtt tcttcttttt
     9901 tctgggaaaa actgttggat gtttggcact agtttttctc cgtctttcaa catatagaac
     9961 aagatgaatg gtgctgtaat gattactacg gttgccgaag cgatcgttcc aacgatcgaa
    10021 cctaaactat tggatactcc actaaggaat tgctgaatga ttgtcccgta agaaatgtct
    10081 aattgctcaa aatatttatt gatatctact tccttgaaca aaggcaattc ggctacctct
    10141 tttaaccagc tttctacctg tttgatgaaa tcaggcatat tagatgccaa tgaggaaatt
    10201 tgctgtacta gacttgggat gacactaagt aaaatccaaa taattgctgc aactaaaagg
    10261 agcagcacaa ttgcgacagc ccatattctt ttgatttttg ctttcatcaa cagcttgaca
    10321 ataggattca ataaatagta aagaaagcct gcaatcagta caggtgcaaa taacgtggaa
    10381 aaaaatgtcc cgatcggttc gaaaataaag tttatttctg atgaaatcat gatcaatgca
    10441 gctatgatca ataattcaag cgaccaaaac attagtttag actgtctaag tttttcaaac
    10501 aatagatttc ctcctttctt tcttactagt atatattata ttcgttaaaa attcttttat
    10561 tcttttttta tttttgaaaa cttgccatac tttttatttg ttttattttc cagtatacta
    10621 tacgaaagga aggtggctag accatgaaag ttgtacaaac aaaagataca atgagcaata
    10681 tttacttaga tgctgtacgg atcaggcatc aagtttttgt agtcgaacag ggtgtgcctt
    10741 tgtctcgaga aatagacaaa gatgaagccc attgtatcca cttcgtcctg tactcagata
    10801 aaaaagaacc gcaaggaact gtacgtcttt tgcctttaga aaacggaaaa atgaaattac
    10861 agcggatggc gattctttct gaatacagac accaagggtt aggaaaaata ttgattgaag
    10921 aagcagaaaa ctttgctaag aatcaaggct ataatacgat tttattaggg gcacagtcca
    10981 cagctgaaac tttttacgaa aaactaggat atacagctta cggcgatcct tttgaagatg
    11041 ctggcatgcc tcatatttat atgaaaaaga ctttataaaa atttatttcg aaaaaagcaa
    11101 aaaacagcaa gcttttgaga aaaatctctc actggcttac tgttcttttt tgtgtttact
    11161 tacgcgacaa ttgcttgtaa cggttatacc aaatatcaat atagccttcc gagaacgggc
    11221 catctccatt attgatccag tcaaccagaa tcttcacatt ttcttttagg atgaagtcaa
    11281 tatcgttcgg gtaattcatc attttgctgt gcatctcata ttcatcgaca tccaatagtc
    11341 gtttctcgcc gtcaggaaat accttgatat ccaagtcata gtcgatatat ttcaaggcct
    11401 catcgtccag caaaaaaggt gaagctagat tacaataata ggaaaccccc ttttctctga
    11461 tcattgctat tacattgaac cagtattttt tatgaaaata aacaatcgct ggttcgcgcg
    11521 ttacccatcg gcgtccatca gattcagtga ctaatgtgtg atcattcacg ccgatcatag
    11581 agtgttcgct tgtttttaat accatggtat ctcgccacgt tcgatgcaat ttgccgtcgt
    11641 gcttgtaact ttggatcgtc acaaactctc cttcttttgg aactcccata gcaacccttc
    11701 cttaagcagc attttgtgct ttgtgtttgg gtgttcatta caccgacatc ataattatta
    11761 tatcaataaa aatgagcttt gcctagaaag gattcctatt gtttcgattt ttctctaact
    11821 gatctaccag tttcatttgg accttaggaa aaacgaccga atcaaatgca tcaggcttca
    11881 accacttcga agttttgttc tcggtaaatt cttgttctgc tccctcggtt gcccgtccgt
    11941 aaaaaaggag aacatgccat ttcagatgac taaaaatatg agtaacttct cctaaatgtc
    12001 gtgtctgcca aacaaagagt tcctgctttt caaatgggag attttgctct tcttctgcta
    12061 ctaggtcatc aaacagatcg atttctggct ttgtttccca ttcttttttc aatcgctcat
    12121 actcctcttg tgtcacttcc atcattggaa atgtccacat attcgccagc aatttttggc
    12181 tatctctttc ttcaaaataa tacgctccag aatgattttg aagagctgca ctgatataat
    12241 aaacgtcttt tggctttgct ttttttgttt ttacaggaaa actcgtttgg attcctcgtt
    12301 tgttagctaa acaaaaggct tggatcggac aagtctcaca ctttggagaa gttggtgtac
    12361 agatagctga acctagatcc atcattgctt ggttgaattc acctggatgc ttttcatcaa
    12421 tgattttccg catcgcttca tcaaagattt tcctgctaga agcttttgcg atgtctgctt
    12481 caatgcaaaa caatctgctg actactcgca tcacgttgcc gtcaactgca ggttctggaa
    12541 gtccaaatgc gatacttgca atcgcgcctg tcgtatatgg tcctattccc ttcaatgagc
    12601 taatttcttc aggcgtttgc ggcatttccc catcaaattc tgacatgatc tgtttagcag
    12661 cagcttggat gttccgggct cttgagtaat aaccaaggcc ctcccatgct tttaaaagct
    12721 tttcttctgg agcatttgct agctcttcaa ttgtaggaaa ccattccatg aaacgataaa
    12781 aataatcgat cactgtgtct actctggttt gctgaagcat gatctcagag atccagatac
    12841 gataaggatc acgattatag cgccaaggca ggtttctttt ttcttgttca taccattgaa
    12901 tgaactgatc ttgaaattct tttgtttctt tatctgtcca tttttcccat tctttattca
    12961 ctttcctctt ccttttccgt aatgaatcgc tggatcatat ccaaatcaaa tcctttttga
    13021 tataagcttt gctttacttt tagatttctt tttttaggtt caaaccttaa atttcttcgc
    13081 catagtttat ctccttgttt gactaaataa tcatattcta tttcttgatc tgcttcttgc
    13141 ggaagatcag ctatcgcttg ttggatcact tcttgagaaa agcctttcgt catcaatgtt
    13201 tgttggattt ttctcaataa ttctttttgg ctttttccgt gattttttcc aaacgctttt
    13261 tcggctgtct gatgtgcatt ttgaacttgg tcttcaaatg tatattggct gagcgcttgt
    13321 tcaatgattt caggtttcaa acctttttgc tgcatctgct gcgctaattt ctttggtcct
    13381 ttgtcactta agcgcatatt cgtgcgcaca taactttccc catagaccaa atcatcgaca
    13441 aggtccaatt cttttaagcg ggctacgatt ttatttcgat cttcttgaag gatttccttt
    13501 tcttttaagt atgttcggat ttctttttcc gatcgcaact gataacttaa gtaattcatt
    13561 gctaggtgaa aaccgaaatc agctccgcta ctcttcttga tctcttggat agtttcttcg
    13621 tctaattccc gtccttttaa taatcgatag cgtactaata catcttcaga tacttgtaaa
    13681 gcttctcctt cagataaatc tacgcgatag aaagggcctt tgcctttact gattctcgta
    13741 acggttaaca taaacatcct cctcgaacat atattctcta tcataccaca gaaaatcccc
    13801 gtaatcaggt attatatgtt agaataaacc acagaaaaaa gaaaaaggga gatacttatg
    13861 gcagaaacaa ttgtcaaaac tggacaaacg gttcctttga aaatcaagcg tttaggcata
    13921 aatggtgaag gaatcggtta tttcaaacgg ttgatcatat ttgttccata tgcactacca
    13981 aaagaagaag tactagtgaa aatcacgaaa gcaactcctc gctatgcaga aggagaatta
    14041 ataaaagtca aaaaaacaag caaagatcgc gtagtggcac catgtcctgt ttactatgaa
    14101 tgcggtggct gtcaattaca acacttagct tatcaagctc aattggattt taaaaaggat
    14161 ctgcttttgc aagcattgga aaaattcaaa ccagcaggct ttcgatctta tcaacttttg
    14221 ccgacgatcg ggatggatga gccttggcat tatagaaaca aagcacaatt tcagttacga
    14281 aaaaataagc agactcaaaa aattgaagct ggtttatacg aagcaaactc tcatcagtta
    14341 gtccctataa cagactgctt ggttcaagaa cctcgaacac aaaaagtaat gaatactgtt
    14401 gttcaactat tgaataaatt tgatgcaccc atctatgatg aacggacaaa cagtggaatc
    14461 ttccgcacaa tcatggttag aatcggtgtt aaaactgggg agttacaagt tgtttttatt
    14521 actcgcagta aaaaattccc gcaaaaaaac gcaatgatcc gtgagatcaa tgaacgactg
    14581 cccgaagttg tttcaatcat gcaaaatgtc caggcacaaa aaacgtctct cgtgatgggg
    14641 gatgaaacga tccacttatg gggaaaagaa gccattgaag aacaaatcaa tgaagtgact
    14701 ttcgatcttt ctcctcgtgc cttctttcaa ctcaatcctc agcagaccga attgctttat
    14761 ggcgaaggaa tcaaagcatt agatgtccag cctaatgaaa cgatcgtgga tgcttattgc
    14821 ggagttggga cgatcggctt aagtgtagca aaacaagcca aagaagtacg gggaatggat
    14881 accgttcctc aagcaatcgc tgatgcaaaa aaaaatgcgg aaagattagg atatcacaac
    14941 actcgatatg aagtcgggac agcagagcaa gttctgccta aatggttgaa tgaaggtttt
    15001 cagcctgatg gtatcatcgt agatccacca cgtacgggtt tggataacgc attgatccaa
    15061 tcattattga aataccctcc aaaaaaactt gtttatatct cctgtaatgt ttctacactt
    15121 gccagagatc tggttaattt aagtaaaaag ttcaagattg aatatttaca gtccgtcgac
    15181 atgtttcctc agactgcacg ttgtgaagtt gtcgtgaaga tgacgagact cgctgactaa
    15241 atgaaagagg ttgtgaaaaa gtctgccctg tatcaagtaa taagaacagc caaacaaaaa
    15301 tagctcttca tgttttatga atactgtact tgtgaccgaa gagttaacag gaggacacta
    15361 tttatttaaa aaaagggttg tgacatagtt tttgtcacag ccctttttga ttattattgg
    15421 atattcacgt ctatcacttg ataaaatgca tttgtcgtat cgctgatatt ccatactgct
    15481 aaaatcacat tatatccttt tcgatcctgc ggaagtgtga tcgtttggtt gactgttttt
    15541 ggtggaacag ttgctttgtc gtctattttt gtgatcaatt caaaatcact aaatttcaaa
    15601 ggttgattag gattccaagt tggtttggta atgtagtaat cccaagtact tgtccgatgt
    15661 tgtgctgtta aattccaagt aatatttaat gttcctggat taaccatgtt tttatgccat
    15721 ctgttagatg tctgttcatc taaaggctca aagccgctga ctcctgcact agcaattttt
    15781 ccatcaatga atgtattttt ggtagcttcg atactttgcg gttcccattg tgctcttccg
    15841 acattttgat ttaagtttcc tgctgctgat gttcctaggt atgcacggct tcctggagaa
    15901 gtaatataac catgcgcaaa acctggaatg ctaaaaccga acaaccctaa actaactaac
    15961 ccaataaaca ataacgtact aatctttttc atgtagaaac ctccttcatt tgataaccac
    16021 attgtaaacg ctttattgta ttaacacaaa cattatgctt ttataatttt atgttgaagt
    16081 aaaaaaggtg aaaaaagtaa gatgcagatt ataacagaag tagacgagaa aagtacaaat
    16141 agaaaacatg agaatcaaat catatgatct ttttcaaaaa catcattcat ataaacaaat
    16201 gttcaacttt ttaataacaa caattatcaa tttttttctt acttcacaaa ctaaatacag
    16261 gtcttgctgt gagccttcat ggtaaactaa tattatagaa aggagttagt aaaatgaaga
    16321 tgataaaaga tattcgtttt tgggtttgtg ttataggaat tgtcatatta ggctttttat
    16381 cagggttgct tagcggaaat cccggaaaat attactattc tttgcaactt ccaccatttg
    16441 caccacctag ctggatattt gggccgatgt ggaccttact gtatatattg atgggtattt
    16501 ctctttattt gttacttaat cacaacaaca aaaaacagcg aaacaatctt gtcggtcttt
    16561 ttgtgattca gttcattttt aattttatat ggtctgctct attttttaat ttaagaaata
    16621 ttttcattgc agcaatcgat attacacttt tagttatttt cttgtcagtg cttatgtatc
    16681 agttgtggct gcatcatcgc ctggcaatgt ggctcatgat tccttattat ttatgggtac
    16741 tattcgcaac tctattgaat tattcaattt attttttaaa ctaagcgcta aaaagagacg
    16801 tcctgtaatt taacacatag aaaaagtgcc acctaagtta gattttttgt ctaatttagg
    16861 tggcactctt tattaaccaa actatttttt tctagtaccc acttgttgtt ccatactcat
    16921 tattctggtc ataaccataa ccattttgtt cctgtccagc gtaaggatca taactttgag
    16981 tatcttgact tggtgtatag tactgacttg tatcttctgt ttgctgataa taggtattcg
    17041 gatcttggta ctgatcatat gtgttttctg aataggaaga ataatcggtg tacccttcac
    17101 tattcgatcc atcaccgttt gtcgaatcgc catcttccga atcatcaaag aacatcattg
    17161 aagcattctc atttttcaaa ttaggtaatg tatcgcttgt ttttatattt aattgtttct
    17221 tcagttcatt ttggatcgaa agaagatcat tttttcctaa aatttgatag tagatatcat
    17281 tgatcatttg tccttcgcct tgcaactggt cttgtttgat tgtttcaaac gccggggtgt
    17341 agttcgtcgc tacatcaagc atgtcatccc aatctaagtc cgtcgtcaca ttgttctcaa
    17401 ctgcttttaa aatttttcta taattgctta cgccatctaa acttaaaact ttattgacga
    17461 ttttcgtcac tacttcccgc tgacgacgtt gtcgaccgat atcgccttct ggatcttcgt
    17521 gtcgcattcg agaataggct aaaccttttt ctccattcaa tgtttgttca cctactggta
    17581 catggacacc atccaaagta aagtcaattt tattattcac tttgatgcca ccgaccgcat
    17641 tgatcaaatc ttccatgcca tccaaattga tcgttacata atgatcaata ggaatatcga
    17701 gcagttgctc aattgaatcc atggccattt cgacaccacc aaatgcgtag gcatgattta
    17761 atttatctac cgttccataa cctacgatat tcgtataaat atctcggtcc aaactgatga
    17821 tcgttgattt tttttgtttc ggattaactg ttactaccat catcgtatca gaacgtcctt
    17881 gttccgttct tcctaatcca ccggtatcta aacctaatag caaaaccgaa aaaggttctt
    17941 tgtcatcaat gcttactctc tcagaacgtt tggaggatat tcgattagag tttttagaaa
    18001 ttttgtttac tgtttggctc gcttctccca taaatctgat tccgtatgct gataatccgg
    18061 caatcagcaa aacgaatacc cctaacaagg ttaatagaac tttttgccat cgcttcataa
    18121 tacacctctg tttcacattt tctaaaatat tgtctcatat aactgttcaa tcagcaaatt
    18181 cactttttaa tcataagaaa actttaaaaa acatttttgt gtaacgatta ttatttttta
    18241 aaatttctca aaattcttaa ctatcatacc ataccgtgaa aaagatcgaa agaccgaata
    18301 cacgcagttc gcaatcgttt tatctttttt tatgtatttt ttccagaaaa agcgaacaac
    18361 aaggtatttt ccaaataatc gtgcagtaaa atgatcgcac atacctttcc catcttttta
    18421 acagtctttg cctaccgttc agattatcat tcgatttcac gacaatttct tcctatgact
    18481 ggtccttttg ttaaacccat cttctattcg ttttatttcc agctcactgc atggctctta
    18541 aaatgactcg aactagtgat tgcattacca tcatctttta atggatagat atgctcctct
    18601 tcaaagtaaa acgattttct tttaaagtaa tagaattgac atggtaatga gtatgtatac
    18661 tagtggtttc acaaatggtc ttttattcaa catccaaaaa ctgaaatttc tttaaaatcc
    18721 attactaacg aagaaaatgt tccttgcttg ataattagtt aggacatgtc aagtgaacaa
    18781 atgcatttac ttagccaaaa aatctaaaaa cttcataaat agacaaaaag acacgactct
    18841 ctaagaagtc gtgtctttat tcaacaaatt attataattt agtcacttca gcagcttgag
    18901 gtccgcgtgc accttcaaca atcgtgaatt ctacttcttg accttcttct aggctcttga
    18961 agccgtctcc ttgaattgct gagaaatgaa caaatacgtc atctccgccg tctactgtaa
    19021 taaacccaaa tcctttttca ttgttgaacc atttaaccgt tccgtgttcc ataaatgtat
    19081 atcctccaaa aaagttcttg caagtaacct tgcaagaaca ttatacccaa tttagaaaac
    19141 gctagcaagc tttcgctctt ttattttcca aattattttt aagactgata tgacggagga
    19201 aatggttatt ttgctgcttc gaaacgtttg tttacttcat cccaattaat aacattccag
    19261 aatgctgcaa tataatctgg tcggacattt ttatatttca aataataagc atgttcccaa
    19321 acgtccaaac ctagaattgg tgtctttcct tccatcaatg gagaatcttg atttgcagta
    19381 gaggtaatag ctaattttcc attttccatt acaagccaag cccatccaga accaaatcgt
    19441 ccagcggctg cttttttgaa ttcttcttta aaagaagaaa aatcaccaaa agcttcatta
    19501 atcgcttctt ttatttctcc tgtaggttcg ccaccagcat ttggtgccat gatttcccag
    19561 aaaaatgaat ggttagcatg tccgccacca ttgttacgta cagctgtctt gatatctgtt
    19621 ggaatagcgt ccatatcaga taataattct tctattgttt tttcgcctaa ttctgggtat
    19681 ttctcaattg ctgaatttaa attcgtcaca taagtattgt gatgcttatc atgatgcaga
    19741 tgcatcgttt cttcgtcaat ataaggttcc aatgcgtcat atgcataagg tagatctggt
    19801 aatgtgtaag tcataaaaaa attcctcctc aatgttttct taataatgtc tacattacaa
    19861 agagtaacaa agagaaggaa attcttcaat tgttatgcct agtcgtcttt ttcactttga
    19921 cctacttctt cttcaaaaac ctcagtttct ttaaaaataa acaacttgct gaaaatatag
    19981 ttcgcaagga taacaataat ttgagtgatg attttcgcca catactcatt gctgttcata
    20041 acattgatca acaataccat tgcccccatg tccaatccgt aagaaaggat acggtagaag
    20101 aagaacttac cgcattcaat aagtgcatgt gtaaaattcg gtgattttga atggaatacc
    20161 caaagtttgt tcgttacaaa agcaaacaat acagaacaaa accacgaaat agaattggat
    20221 aaggccatac ttaaatcaaa atattggcgt gcgagagcaa aactaacaaa atttacaatc
    20281 gtagccaaac caccaaaaaa gagatagact agtatctccc aatatccttt tgcttctaaa
    20341 tatcctttta actgtcgata ctgcttcatg agccaaccta ccttattaaa aattcttatt
    20401 tatcatactc tatatttcaa taactttcat ctttatttaa aaaaactaat tttttttgat
    20461 cctttgcctt tgtctctgct tggatcgtca catgtgtaat accatatttt aattttaata
    20521 aagaaattat acgaggatat atcttttcaa tctcgcttag tagctgatct tcaaaatcaa
    20581 tatgtccgtc gaaaataatt atcttttcgc tatactgcca aatatggaca tgatgaacat
    20641 cctttacccc atcgattgct tcaatttctt gttccatttt ttcatagtcg atagcaggtg
    20701 cactttgtat caaaatagcg gcagcttttt tgatcaccat tatcgcttct atcaatatat
    20761 atatcgaaat gatcagtgta ataactggat cgatccagta gatatcgaaa aaacggatca
    20821 aaattgctcc gataatcaca cttaccgaag acagtgtatc acttaacaaa tgcaaataac
    20881 tagattttat attcaaactt tcatctgctt cttgtcgtaa taataatact gagaacaaat
    20941 tcgcgagcaa tccaataact gctactgtta gcatcaaatg actatttatc ttttctggtg
    21001 aagtgaaccg cttgaatgct tcaacggtta aaaacaagga taatatgatc aatatgaatg
    21061 agttgatgaa agcactcaat atttctaaac gctggtagcc atacgtacgt tttttatttg
    21121 cttcttttcg ggccattttt tgtgccccgt aagaaaaaat aattgccaat gtatcactaa
    21181 ggttatggaa agcatctgaa acaagagaca aactccctga cataatccct ccaataaatt
    21241 ctgataaagt gatcaccgca ttcaaaacca ctactccaaa tagttttttt gatgatctct
    21301 gtggctgtgt aggcactatt ccttcctcca ttttatttat attggatagt ctttagacaa
    21361 gaataacatt tttttgatat ttcaccaatc attcagctgt ttccttaaag cacttgtatt
    21421 tcacttgata cttcgctaga attgcaagtg atgacactac tagatgagga gtacttatga
    21481 caacaaaaca attaattatc agttccttta tacgattcaa agacctaaag gaggcgagaa
    21541 atgtcccatt ttggaaagcc attgtctatc ttttagtttt gagtataatt atggctttgc
    21601 caatctcttt tcaagttttt caagtcttaa acaacattca attggatggg caaaagattg
    21661 ctactcgcat tcctgatttt acaatccaaa acgggcagct tcaaaccgaa gaaaaagaag
    21721 gattcattta ccaaacaaac tccattatct tcacatttga tccagaggga aaacgaacag
    21781 aacaggacat ctctactgat ttgatgggga attttgttag tgtcgggatg ttaaaaaaca
    21841 aacttatcat agctcttcct aatacaggca cgacttctgc tttgttgaac aataaccaat
    21901 tagaacttcc ctacaccaat gaatctttaa aaaacttaac aggtaagcag ctccgttctt
    21961 ttttaagtga agcatctata ccactttgga taaaagtcct gacatttata ttttctattt
    22021 atcctagttt tctgaatctg ctgatcactt tgctatttac caacattgct gcttttctct
    22081 atgcacgctt tcgactaaca aaagctactt ttcttgattg tttaaaaaca ttgatctatt
    22141 caatttcatt gcctactata attgcaacaa tcttgatggt ctttcttcct tcttttgaca
    22201 ctagcgcctt cattgctttt gcaggaatct ttatttttgc tcaagctgtc aaaggatggg
    22261 caaaaatatc gatcaattaa gaaataaaga caactcgtta tttctacttc aatagaccct
    22321 tttagaggtt gtgacatttt tttgtcacag cccctttttt atttgaaaaa tatttctttc
    22381 tcaaaaaaca agcaaggcgc ttcaaagttc ttcttgtatg cgctacactt agagtaaaga
    22441 aaggcgggaa attaactatg tcagaaagaa tcatttatgc agatcgtcca tttcgtgatg
    22501 aatttatcga agaaatccat actatagcgc ctggttatat ctttaaaaca gaattacaac
    22561 cagaggatct acaaaatgta gagatcagtt tgggctggaa ctcagactac caagaaaaac
    22621 tattagcctc ttctaccttg aaatgggtcc aatctatctc agctggtgtt gatcagctac
    22681 cccttgagac tttttcaaag aaacatattt tactttcaaa tggtagtggc atccacagtg
    22741 aatcgattac ggagcatgtc ttaggaatca tccttgggta ttctcgtggt ctttttcaag
    22801 cacaaaaggc acagagtgac atgaaatggt taggttcatc ggtccattac caatctatcc
    22861 aagggaaaaa aatactaatc attggaactg gccatattgg aaaaatgatt gcaaaaaaat
    22921 gcaaagcatt agacatgaac tgctatggaa tcaatacatc cggtcatcca gcagaaggaa
    22981 tggaaaagac tttttcttta aatcaactaa aggaagttct tcctgaagta gatttcgtag
    23041 tcaatatctt acctctaacg gaggaaacaa ctgggctctt tgatcagcat atgtttgaac
    23101 actttgatcc aaaatctgta ttcatcaatg ttggccgtgg tgcttctgtt aagacacaag
    23161 atctggtgca ggctttaaat aaccagcaac tttcctttgc agcattagac gtttttgagg
    23221 aagagcctct tccacaagat catcctcttt ggaaaatgga caatgtactg attacttccc
    23281 acattgcagg tttgacacct gattttcaaa agaaattaat ggctattttt ttaaccaatc
    23341 tgaaaagcta ctttgccacc catgagcttc aaaccaatca agtaaaattg tctgcaggat
    23401 attaatagct gaaaacaata ttttca
//
LOCUS       DAZKLD010000030        23342 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.030, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000030 DAZKLD010000000
VERSION     DAZKLD010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23342)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23342)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23342
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.030"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            210..1016
                     /gene="proB"
                     /locus_tag="ACG8KR_000980"
     CDS             210..1016
                     /gene="proB"
                     /locus_tag="ACG8KR_000980"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HFQ0200372.1"
                     /translation="MRKEIKNAKRIIVKVGTSTLIYPNGNINLAAIDQLAFVLSDLRN
                     RGKEVILVSSGAVGVGMHKLHLSERPTLIPEQQAAAAVGQAELMNIYNQRFSVYNQQT
                     AQILVTRDVVDYPESRKNVRNTLEQLLIMDTIPIVNENDTVAVDELDHLTKFGDNDQL
                     SAIMAKLVAADLLIVLSDIDGFYSDNPTTNPEAQMYRYIDKIDEKILAQAGGAGSTFG
                     TGGMQSKLKAASRIFEANSAMILANGKNPAIIFDILAGKEVGTLFKEDLK"
     gene            1013..2260
                     /locus_tag="ACG8KR_000981"
     CDS             1013..2260
                     /locus_tag="ACG8KR_000981"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HFQ0200373.1"
                     /translation="MTDLMQLGQQAKDSAQQLALMPTNRKNELLVQMAQTIKKNQQAI
                     IEANHKDLEKAVENNISETMQDRLQLTPERINAMATEIEKIASLDDPIGKVDEMWTNT
                     DGLRIGKKRVPLGVTGIIYESRPNVTTDAASLAFKSGNAVILRGGKEAFFSNQLLVQL
                     LQQVLVSAGESPYAIQFVDDTSHETAGQLMRLTEYLDVLIPRGGAKLIQRVKEQATVP
                     IIETGTGNNHVYIDKEAQLSMAVNIIVNAKASRPSVCNAAETLLIHSEIAPFFLPAIE
                     KELVEHGVSLRADARALEYLETAALAEDTDWDTEYLDYILAVKVVDSLDEAIAHINRH
                     NTKHSETIVTDSYAASQRFLNEVDAAAVYVNASTRFTDGSVFGFGAEIGISTQKLHAR
                     GPMGLNELTSTKYIIYGDGQIRE"
     gene            2336..2680
                     /locus_tag="ACG8KR_000982"
     CDS             2336..2680
                     /locus_tag="ACG8KR_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0200374.1"
                     /translation="MFEILMIFFIYLISLNIAAFLGVGILSLFFQFKKRSIGSQREKW
                     SQYFDKIGQKGLVTRLHISYMVALCLLATINYYSFFDHSIAYTITLLIAGIFHLSYKY
                     QLNKNHLNRTFR"
     gene            complement(2797..3123)
                     /locus_tag="ACG8KR_000983"
     CDS             complement(2797..3123)
                     /locus_tag="ACG8KR_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0200375.1"
                     /translation="MLEEYFQEVGLTLVELPIEFSDDRLIYRGYIKELPIINAEGETR
                     KAMQQKIAGMYQVYREQLLAESAEEEKEKTMNLSMDELLRYYDGETFDGFSLLNEEET
                     DLPGSW"
     gene            complement(3496..4697)
                     /locus_tag="ACG8KR_000984"
                     /pseudo
     CDS             complement(3496..4697)
                     /locus_tag="ACG8KR_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            4814..5329
                     /locus_tag="ACG8KR_000985"
     CDS             4814..5329
                     /locus_tag="ACG8KR_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFQ0200376.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            complement(5528..5974)
                     /locus_tag="ACG8KR_000986"
     CDS             complement(5528..5974)
                     /locus_tag="ACG8KR_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HFQ0200377.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(6021..7499)
                     /gene="msr(C)"
                     /locus_tag="ACG8KR_000987"
     CDS             complement(6021..7499)
                     /gene="msr(C)"
                     /locus_tag="ACG8KR_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HFQ0200378.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRPDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            complement(7853..8482)
                     /locus_tag="ACG8KR_000988"
     CDS             complement(7853..8482)
                     /locus_tag="ACG8KR_000988"
                     /EC_number="1.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016657 - obsolete oxidoreductase
                     activity, acting on NAD(P)H, nitrogenous group as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFQ0200379.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMQPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITTFE"
     gene            complement(8466..8930)
                     /locus_tag="ACG8KR_000989"
     CDS             complement(8466..8930)
                     /locus_tag="ACG8KR_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFQ0200380.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIEH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            complement(9417..10685)
                     /locus_tag="ACG8KR_000990"
     CDS             complement(9417..10685)
                     /locus_tag="ACG8KR_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HFQ0200381.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            complement(10916..11653)
                     /locus_tag="ACG8KR_000991"
     CDS             complement(10916..11653)
                     /locus_tag="ACG8KR_000991"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFQ0200382.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            complement(11720..12289)
                     /locus_tag="ACG8KR_000992"
     CDS             complement(11720..12289)
                     /locus_tag="ACG8KR_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HFQ0200383.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            12417..13289
                     /locus_tag="ACG8KR_000993"
     CDS             12417..13289
                     /locus_tag="ACG8KR_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFQ0200384.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKVFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            13631..14503
                     /locus_tag="ACG8KR_000994"
     CDS             13631..14503
                     /locus_tag="ACG8KR_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFQ0200385.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            14500..16458
                     /gene="nagE"
                     /locus_tag="ACG8KR_000995"
     CDS             14500..16458
                     /gene="nagE"
                     /locus_tag="ACG8KR_000995"
                     /EC_number="2.7.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015572 - N-acetylglucosamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-specific PTS transporter
                     subunit IIBC"
                     /protein_id="HFQ0200386.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAATRPAQTNTVVKTETFAGGTIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVKEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(16790..17641)
                     /locus_tag="ACG8KR_000996"
     CDS             complement(16790..17641)
                     /locus_tag="ACG8KR_000996"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFQ0200387.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKKLEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            17764..18231
                     /locus_tag="ACG8KR_000997"
     CDS             17764..18231
                     /locus_tag="ACG8KR_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HFQ0200388.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            complement(18494..18976)
                     /locus_tag="ACG8KR_000998"
     CDS             complement(18494..18976)
                     /locus_tag="ACG8KR_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFQ0200389.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVIA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(19076..19939)
                     /locus_tag="ACG8KR_000999"
     CDS             complement(19076..19939)
                     /locus_tag="ACG8KR_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFQ0200390.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGARLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYELVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
     gene            20537..21412
                     /locus_tag="ACG8KR_001000"
     CDS             20537..21412
                     /locus_tag="ACG8KR_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HFQ0200391.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPQFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKTKETVKKDDFNEK"
     gene            complement(21557..21709)
                     /locus_tag="ACG8KR_001001"
                     /pseudo
     CDS             complement(21557..21709)
                     /locus_tag="ACG8KR_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="xylulose kinase"
     gene            complement(21977..23308)
                     /locus_tag="ACG8KR_001002"
     CDS             complement(21977..23308)
                     /locus_tag="ACG8KR_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFQ0200392.1"
                     /translation="MGKSSRKDIVFEKFGIVATKLGNQIHLRTLRDAFATFMPFMMLA
                     GFVTLINSVILEPTGFMGEIVDTSTLTTLQQIGTSIANGTLSITTLLVVAAVSYHMCA
                     SRNYTNHIAAVLVAISTFVVLTPMQMMFTPENADKAVEVTGVIPISYTGASGMFVGIF
                     VGLAATELFIKLSNNKKLQIQLSGNIPPAVLKSFNVLIPIIITITSFALLSFIIIQLF
                     NMDINKLITTIVTEPLSKITTGLPGFLLITSIANLFFGFGIHQAVISGSLLDPFLIQN
                     MQENMAAYANKEQIPHIINMAFKDTFAVMGGSGNTIALLIAIFIFSRRKDYKDFAKLS
                     VTPAIFNISEPIIFGLPIVFNISLIIPFVLAPIFSLTVAYFATAVGLINHVVVQIPWT
                     TPPVISGFLATAGDWRAAVLQILIIAASVFIYLPFLRIDEHVTTKLSENET"
BASE COUNT         7176 a         4725 c         4153 g         7288 t
ORIGIN      
        1 actactccta ttctaacata tccgaggctg taaaagactt tctttacaaa ttcttcaaga
       61 aattctgcta tcttaaagta aaccattaat tcgttctttt tccaaacgac tgcaagttgt
      121 acaaaaaaac tattttttac atgactttac tcgtcaaaac aagtagaata gaacgaaatt
      181 aatgagtgga tatttgattg aggtgtcaca tgcgtaaaga aattaaaaat gcaaaacgaa
      241 ttattgtcaa agtaggaaca agtacattga tttatcccaa tggaaatatc aatcttgcgg
      301 caatcgatca gttagctttt gtcctttctg atcttagaaa tcggggaaag gaagtgatct
      361 tagtctcatc aggagctgta ggcgttggta tgcataaatt acatttgtca gaacgtccga
      421 ctttgattcc tgaacagcaa gctgcggcgg ctgttggtca agccgaactg atgaatatct
      481 ataatcaaag attcagtgta tataatcaac agacggcaca gatcctcgtg acaagagacg
      541 tggtagatta tcctgaaagt cgtaaaaatg tgaggaatac attggaacag cttttgatta
      601 tggacacgat ccctatcgtg aacgaaaacg atactgtagc agtggatgaa ttagatcatc
      661 tgacaaaatt tggcgataat gatcagctgt cagccatcat ggcaaaactt gtcgcggcag
      721 accttttgat cgtcctttct gatatcgacg ggttttattc agataatccg actacaaatc
      781 ccgaggctca gatgtatcgc tatatcgata aaatcgatga aaagattctg gcacaagcag
      841 gtggtgctgg tagcacattt ggaacaggcg gtatgcagag taaattaaaa gccgcttcta
      901 ggatatttga agcaaacagc gcaatgattt tagcaaatgg aaaaaatccg gcaattattt
      961 ttgatattct tgcaggaaaa gaagtaggta ctttatttaa ggaggacttg aaatgactga
     1021 cttgatgcaa ttaggacaac aggcaaaaga tagcgcgcaa caactagcac ttatgcctac
     1081 aaaccgcaaa aatgagctac ttgttcaaat ggcacaaacc atcaaaaaga atcaacaagc
     1141 gatcattgaa gcaaatcata aagacttaga aaaagcagtc gaaaacaata tcagtgaaac
     1201 gatgcaagac cgcttgcagc tgacacctga gcgaatcaat gccatggcta cagagatcga
     1261 aaaaattgct tcgttagacg atcctattgg aaaagtagat gagatgtgga cgaacaccga
     1321 cggattgaga atcggcaaaa agcgcgtgcc actaggcgtg accggcatca tttacgaatc
     1381 tcgtccaaat gtcacgacag atgcagctag tctagcattc aaatcaggga atgccgttat
     1441 ccttcgcggt ggcaaagaag cattcttctc gaatcaatta ttagtccaat tattgcagca
     1501 agtactggtc agtgctggcg aatctcctta tgccatccag tttgtcgatg atacttctca
     1561 tgaaactgca ggacagttga tgcgattgac agaatactta gatgtgctga ttcctcgagg
     1621 cggagcaaaa ttaatccaac gagtcaaaga acaggcaacg gtacctatca ttgaaacagg
     1681 tacaggaaat aatcacgtat atatcgataa agaagcacag ttatctatgg cagtgaacat
     1741 catcgtcaat gcaaaagctt cccgtccatc tgtctgtaac gcggcagaaa cattgctgat
     1801 ccattctgag atcgcacctt ttttcttgcc agctattgaa aaagaactgg tagaacacgg
     1861 ggtctcttta cgtgcggatg ctcgtgcgtt agaatatcta gagacggctg ctttagcaga
     1921 agacacagat tgggatactg aatatctaga ttatatcctt gctgtcaaag tggtcgattc
     1981 tttggatgaa gcaattgcac atatcaatcg gcataatact aaacattcag aaacgatcgt
     2041 gacagatagt tatgcggcta gtcaacgttt cttaaatgaa gtggacgcgg cggctgtcta
     2101 tgtcaatgcc tccactcgtt ttacagacgg atcggtattt ggttttggtg cagagattgg
     2161 tatctctact caaaagttgc atgcccgtgg tccaatgggg ttaaatgaat taacttccac
     2221 aaaatatatc atttatggcg atggacaaat ccgcgaataa tgtttcgtgt gaaacattat
     2281 tcccgccttt tgcctttttt ctgtaaaggt tctatccaac tagaaaggtg tcaatatgtt
     2341 tgaaatttta atgatttttt ttatttacct tatttctctg aatatcgctg cttttctggg
     2401 agtcggtatt ttatcccttt tctttcaatt caaaaaacga agcataggga gtcaacggga
     2461 aaaatggtcg cagtattttg acaaaatcgg gcaaaaaggg ctggttacac gcttgcacat
     2521 tagttacatg gttgcattat gcttattagc gacaatcaat tactattctt ttttcgatca
     2581 ttcaatcgct tatacgatta cgctactgat tgctgggatt tttcacttat cttataaata
     2641 tcagctgaat aaaaaccact tgaatcggac tttccgctaa aaaatatccg tacgataagt
     2701 agagtgcctt gaagatcggc aaaaaactgc cggacttttt agaggctgtg acaaagattt
     2761 tgtcacagcc tctttttcca tataaacggc gttcgcctac cagctgcctg gtaaatctgt
     2821 ttcctcttcg ttaagaagcg aaaatccgtc aaatgtttcg ccatcataat aacgaagcag
     2881 ctcatccatg gacaagttca tcgttttttc tttttcttct tccgctgatt cagccagtag
     2941 ctgttcccga tacacctgat acataccagc tattttttgc tgcatcgctt ttctagtttc
     3001 accttccgca ttgattattg gcaattcttt gatatagcca cgataaatca accgatcatc
     3061 tgaaaattca atgggtaatt caacgagtgt caacccgact tcctgaaaat attcttccag
     3121 catctattat ccccccattc tttttcttaa atgaatttta gcttacctac tgaaaatttt
     3181 ccagtttcat tattttgaga aaaaaaggga atgtgacaaa aatcgtgtca catccccttt
     3241 ttccgaataa atggtgttct tctgccaact cctcaatcat aagtcacaat attcaacaaa
     3301 catgagaaac tgttttttga aattgttcct tgtgattcag agttaaacga cagtctcaca
     3361 acctcttggt aaacggcgtt caaaagcttg catctgcggt aataagccca accaaacgaa
     3421 aaatatgaga agctattttt gtttggctgt tcttatgact tgatgcagga cagctttttc
     3481 acaacctctg atttcttatt cttcttcatt tgtagcaaaa taattacggt gataaatgat
     3541 acaacataca ctgataagaa caatggcacc tgcaaaataa aacgcatagc tttcaccaat
     3601 atgttcacca atcacacctg caataaatgg tgctaaagct ccaccgaaaa agcggacaaa
     3661 actatagctg gacgaagcaa cgtttcgttg cagacctggt atttccatag caatcgtcgt
     3721 caacagtgta ttgataattc cttggaaaaa tcctgctaac acaatgctga tcggtacaag
     3781 gactggcata gaagttccag ctcctaataa cagcaaacaa ataatgaata agacaaatgc
     3841 tgctaaaatt gtccgataag tcgtaaactt ccgctcaaga gccggtgcaa cgaaaatcga
     3901 agcaatagcc agaagcacac cccagccaaa aaatacaaaa ccgacttcta attcagaata
     3961 gccaatcaat aaaaatggcg catacgcaag taaagtaaag aatccgaaat tatataacaa
     4021 tgcgattagt ccaactgaac gaagtttatg attgcttaac gctttgacac cttcccaaag
     4081 gcctactttt ctctttggtt tctcagtttc aggtaaaagt accgctaggg ctataaaacc
     4141 gcaaaacatc aaaaatgcta ccccaaaaaa tggataacgc catgaaccac tgcccaagac
     4201 cccaccgact agagggccaa cagccattcc gcagccaagt gctgcttcat acatcatgat
     4261 tgctttttct gtgttaccaa ctaaaatact gacgatcgtt gctaaagcag tagagacaaa
     4321 taaagcattc ccgacacccc agccggcacg taacccaatc agcattccga tattcccaga
     4381 taatccgcca agaccagcga atacaacgat gataaacaat cctgtcatca atgttttttc
     4441 aaaccgatcc gactagataa gaaacctgta aatagcatca ccacaccagt gactaacatg
     4501 taactagtaa ataacagggt cgtttcagct ggcgtagcgt ttaactgtgc agcaatcgtt
     4561 tttaagattg ggtctactag acccactccc ataaaagcga tcatacaggt agctgctaca
     4621 actagcgctg aacttttccc ctttgtttct tcatttgttt gttcttgtat atctatttga
     4681 ttctccaata tttccatccc tttctctcta ttagcatcat acatgattta ttttacatgt
     4741 aaatatgcac atgtaaaacg tggaaacttt actgtgatat aataaagaaa ataagacgag
     4801 aggtaggatt tttatgaata ctttagccta tatattatta tgcgtgcttt tatccaaacc
     4861 acgttctggg tatgaattga agcagctgat cacaattttt tgggaagccc atcacagtca
     4921 gatctataca acgcttgcca agttagacca gcagggttat gtgaccgttt tggacaaaga
     4981 cgagcactct caaaaaaaga tctaccactt gactgatgaa ggacgtgctc ttgtagagga
     5041 atggatcaaa gaggaaactc ccgctcctac gcaaaaagat gaatttctag caaaaatcta
     5101 tgctttttcg acgctcgata aaacaacagc tactggactg ctattcacta gagaacaaca
     5161 gctgaacaaa atcatgcaaa aaaatcaaaa aaaattgaat gacctctcgc tagacaagaa
     5221 acaagaattt ggacgttatg ttgtgatcca gcgcagaatt atgctttgtc agcaggaact
     5281 ccaatggtgt cagcaagtaa gagaacaaat gcagcagttt tttgagtaag ggataaacag
     5341 aacctttcta gaaatatcta ttgaagagca aattatcttc aataaaagag caatacagtt
     5401 ggaaaagaaa aagcgatccg aacgacaggc caaaacctat cgttcggatc actatttttc
     5461 tagaactgca ttcaagagac tatgacaaaa agcatgccaa ggcctttttt caaataacag
     5521 tgttccatca ttcattactt tgaattgctt ttagcacact tcttgaaagc gggccaacga
     5581 tgatcaactg caacggtaaa gcggcaatca cattcatttt ccaagcattc atataagcag
     5641 agaataattg atcaggtatt ccttgttcca tgatcacacc gaataaagac atacatgtca
     5701 ccattcctaa aaccatcaag catgaaatag ttaagatcat ttggaccttg ctttctggat
     5761 ttactggtag tttaaaggca atttttttcg ccagcacacc gatgatgaag acatccaaaa
     5821 tgaaagccac cacgaatcca ggcactagac caatcaacaa acttgataaa tgaagatctt
     5881 gatgcagcca taaattatac gcgctcatcc ccaagaccat caaaaaacac atcattgttg
     5941 taaaaattat tccttctttt ttatttgtag gcaatctgta ccactccttt aaatttgtca
     6001 cgaaaatcat cgtagcacat ttaaaaattt ctcgtaagta ctttttttat tttctgccct
     6061 tttatttcat aaacttcatc agccgtacgc tcgacaaagt aagaatcatg agaagtaaac
     6121 aaaatcgttc ccggatatat ttgcattagc ttctctaaag cttcgatcgt tgccatatca
     6181 ataaaattag tcggttcatc taacagcaac acattacttg gcttagtaaa aagcaaagca
     6241 atcgtcagac gggtcgcttc tcccccactt aatgtacata acggtttcgt caaagtttcc
     6301 tgagcaaacc ctaagtgatt cagcaaacta cggacaagcg attctgaaaa ctctgtttgt
     6361 ctcatgacaa aacgaatgat ggattcttca gatgtcattt gataaccctt ttgctgatat
     6421 acttgaaagc ttactttcgg agagagaagg attccttctc cttgtttgcg gatatgttct
     6481 aataaggttg tcttacctga accattttca ccgacaatcg cgattttttt acctaaagaa
     6541 aactgaaaat caccatttac cagtaatgtt ctcccacttc tttccaattg gacattttgt
     6601 cccatgatgg gatagcggct gtggagttca agagattttg gtactggaaa acggatttgt
     6661 ttcatctgct gcggcttggt tgtttctcct attttttgga gtcttctctc taatgttttc
     6721 gcattctttt ggatggcttt ttgtacactg tctttttgtt tagaggaaga caaccgatcc
     6781 ggtctgattt gttgcttttt ctttttactg gacacttttt ccaacttttc cgcttgtttt
     6841 ttcttttctt ggatcgattt ttccaatcgt tttttctctt tcatgatcga ctctgcttct
     6901 cgtagctgag tctttttctc caattccttt tgagtgagat aggcactata attccccgaa
     6961 aactctcgga tttctccgtc ttttacttcc cagattttcg atgctagctc atctagaaaa
     7021 tagcgatcat gactaacaaa aaccagcgta ccataataat aacgaagctc ttcaatcagt
     7081 cgatcaatac tttttctatc tagatgggtt gttggctcgt ctagaagcat cccttgttca
     7141 taagaagaaa gaagacgtat caactcgatc tttcgttctt cccctccgct ccatttccgc
     7201 tctgaagttt cttccagtct taactggaaa tgactggcta attccaaatt taggtccttt
     7261 gcttcagcga gataggttaa ttgaggcagg tagttgaagg tgatctctgt ttggattttc
     7321 ccatgatcgg gaaaaagttc accggctatt agtttgagca aagtcgattt accgacacca
     7381 ttttctccta tgatccctat acgatcttgc tgatagacac gaagagaatc aatagatagt
     7441 tctagctggt tgccaaagct gacttgtaga tttgttatat ttactgctaa attttccata
     7501 tgttttacac tcctatttca tgtaaaacaa caattatcga tactaaaaaa gagcagaaaa
     7561 atctgtttgc aaaaaaactg catcacatct tttcctactc tgaacgaaat aactatgact
     7621 aaatcaccaa atctgtcata gctttcacct attctactgt cgttcgaaaa aatggacata
     7681 ctccatccat gaaattgttg tttatacatg tcttttgaat ttatttagtt gttattcaaa
     7741 agttcgaaac gtaatttcat cgatgcagtc atttttccga cctccttttt tattaggtta
     7801 ccacacacga ttcaaaaaaa gcaatagcta ctgaatagta actgaaaacc ttttattcga
     7861 acgtagtgat cttttctggt gctaaacgaa cagactcgta accttcttct accgcttttc
     7921 ctattgataa aataataact ggaacataac ggtctttatc caaaccaaat gcctctgcca
     7981 actgatctgc ttcgaatcca ccaattggat ttgtttcata gccatgatca cgcgcaacta
     8041 acatgaattg catagccgct aagctagaat cgactttgac tacatcgttc atttcttctc
     8101 ttgaaaaact cttgtaataa ggaatgatcg cagctaattg ttgatctctg acttcttttg
     8161 gcatcttccc tgattcgtaa gcttggttat agatttcttc gccatattcg taacattgca
     8221 tatcgccaaa aatcatgacc attgcagaag aagtatcatt ttgtctcgta ttgaaacgga
     8281 tcaaaggacg caagatttct tttgcttcct cgctttcagc gaccacaaag cgccatggtt
     8341 gcatattgac agaagaaggt gcagtcgtcg cttctttgat catttccagc atttcttcgt
     8401 gtgggatttt gtatgtttca tcatatacgc gaacggatct tctaccagtc ataattgttg
     8461 aaaaatcatt tttttgttcc attattatta acctctttcg tttctgttgt tatattgcaa
     8521 agttgggtat tcaatttatc taaaagtgtt agaaattgtt tcatttctac atcgctgaaa
     8581 gccggggata gcacggaacc tgtatcttta tggtccattt cacaatgctc gattttcttc
     8641 tgggcttttt ctgttagcgc tacatacact tcccggttgt tctgaggatt tctctgacgc
     8701 gaaacatagc catttgcttc caataccttc agatgacgag taactgctcc ttgatcaatc
     8761 tgcaactttt cttgcaaact gctttgtagc aatggatttt cttctttcag tctcatcatg
     8821 atctcgtatc tagtaagact tataccgata ttttcttcaa agatctggac gattttttga
     8881 tcggctaatt tgatttgata taaaaggtga ctaatctctt ctaaagacat aatttcctcc
     8941 tttctaataa ttgactcgtc aacgattgac ttgtcaataa tagaataaaa aaagaaaaaa
     9001 gtcaaagaat cagctgtgta ataaaaaaaa cgctctcaat agaaaagtat agagaaaaga
     9061 aattttttgt agaatgaacg gaaaaaatga taaagaaagc acaacgctaa aaccaagatt
     9121 attgattctt atcaaactat agaagatagc tcttctgcag tagaaaattt tacaccgcca
     9181 aaaatttccg aaatcgcctt ttgcaagaat cccgtcgaat attagaaatt ggatcagtat
     9241 gcttaaacag taataaaaaa gttatgacaa gatgttgtca caaccttttt tgagtaaacg
     9301 gtgttcaaaa acttgcatct gcggtaataa gttcaaccaa acaaaaaaca tgaaaatctg
     9361 tttttgtttg gctgttctta ctacttgatg cagggcagct ttttcactac ctctttttaa
     9421 taaccataaa atcctattcc ttcatatctc cagcccatcg gtgttagcca atctctttcc
     9481 ttagcactca aggtatagtg atgggtacca gttttttcat ttggattaaa tagtcgataa
     9541 attggcttgg aaccaccaga ataaaaactt agaccttcat aacgccaacc gatttttgtt
     9601 aaagagttga tctcagattg tgccattgtg taatgatggt ctccactgta aggattgtat
     9661 aaacggtaga caggttgtcc gctgtttggt gcttgccaag caatcccttc atattcccag
     9721 cctacagttg gcagataatt tttttctccg gcattcaatg tatataaatg ttctcctgta
     9781 tgacggttat acagacgata catcggagaa gttgaagttg gtgctgtatc atactgtgtc
     9841 agattatagg tttcgattac attgttcaat gtagaaccat aattaggcgc agtggcataa
     9901 cgacctgtta gccaagccgt agcatctttg taagagttcg tattgctttt ccatgcaccg
     9961 gaatagtagt aaacgcctgg ctgaaaagaa gtcgtcttca ataccgttac gttatcttgc
    10021 aatgattctt tgtacgaagg atattttcta aaagcggcat tcaccgtaat ccattgccca
    10081 ttcacatatt ccgaagtagg catcgtaaca gattggccat tatagcttcc tttaatccca
    10141 aatagattgt aattaggggc agaagccaaa gtactgttcc cccagccact ttcaacgatg
    10201 gcttgagcaa tcatgactga tgcatataag tcattggcac ttgccaaact ttgtgcagtt
    10261 gaggcaatac tatcaatgaa cgcttgttga gaattagcag atgagcgagc ataaaaagca
    10321 tagccagtat agttttcgaa ttcagacaaa tctgcttgat gggtatgtgt caatgtttgc
    10381 ccatctgtca attggttcgt gatttcttct gcattctctg gattgttctg agcttctgct
    10441 gatgtactgt cttcggtttg tgtttgttct gttgtttcag ttgttggttc ttcggttgaa
    10501 attgtatttt ctgagacagt agctgactgc aaagtactct ctgtatcgtt ttcaattcct
    10561 gtttctgaag aactcgatgt ctgagttgcg acagtttctg tcgattcttc agctagcact
    10621 gttgtgttgg caactgctag atttcctaaa agaataacgc taaataataa attctttgct
    10681 ttcatctttt ttctcctttg aattatgaaa cttttcctaa ataacagtgt aactaaaaaa
    10741 gtaaaagcaa gtcaatgtcc atatttaaat aacatttgtt tctttttgat aaatttgctt
    10801 tttctacata aaaagacgta aatgaagaat gttcttctcc attcacgcct taaattgatg
    10861 gtcgtttgaa taactattta acaagtaatt ctctttggat attatccgtt atggattata
    10921 cttcctgtgt cgtcggacgg ataatcatgt tactgacaga catccggtct ggcgtttcta
    10981 tcgcaaatgc tacagcagaa gctatatctt cggaggtcaa tccccattct ttttgcgctt
    11041 catgaagttc aagagcagcc tttttgtcgc tgatggtttg atataattca gtttgaacag
    11101 ctcctggaga gatgatcgta gatttgatgt tattttgccg ttgctcttga cgtaatcctt
    11161 ccatgattgc acggacagca aattttgttc cgcaataaac ggcggaatca ggataaacaa
    11221 catgtcccgc cacagaatca gttgaaatga tatgcccaga tttttgtttt tccatgattg
    11281 gaagtacggc ggaaattcca tttaacacac ccatgatgtt gatatcaagc atattttgcc
    11341 attcatcacg gtgaccttca actaatggag cggttggcat gattcctgca ttattgtaca
    11401 atacatcgat tcgaccgaac ttttccatcg ctaaatcgat aactgcttgg acttgtgcaa
    11461 aatctcttac atctgctgtt tgaatatata gctcagcttc tggtaaagat tcttttattg
    11521 cttttaggcg atcttctcgg cgtgcagcta taactagttt agcccctttt tctgctaata
    11581 gcttcgttgt tgcttcaccg attccgcttg aagcgcccat gatgacaatc actttttctg
    11641 ataaatcatt cattctattc tcctcctgtt ttgaaaaatc tcttgcttat actcactatc
    11701 tcttatttca tcttactgtt caaataatcc gttcgacagg tttgacgatt ttggcaggga
    11761 ttcctgcaac gatggtattg tctgggacat ctttcgtaac agttgcattc gctgcaacaa
    11821 tggcattctc gccaatcgtt acgcctggca gaatagttgc tcctgctccg atccaagcat
    11881 ttttcttgat gtggattggc tttactgaca agcctcttcg ttcttttgga tcggttagat
    11941 ggttcaccgt gatgaggttg acacgaggac caatcaatac tgaatcttct aaacagatgc
    12001 cacctaagtc aacgaacatc acttgacgat tgataaagac atttttccct atactaatat
    12061 gtttgccgaa atctgtataa aaaggcagca gaacagtcac cgtatcatct actttgtcac
    12121 taatgatatt ttctagccgt ttgcggatat ctgctggttc atggtaccca gtattcagcg
    12181 ctgctaaagg ctcttcgttt tcagcttgga tctgatggat cacttcaaag agcggatccg
    12241 ttactcgaat ctctttattg acgatttgtt tttcgatgag gcttgtcatt ttgctccctc
    12301 cttttatcct tactttataa cggacggctt atcatgtcta atacttataa aacatactta
    12361 actattcctt ttaactatac ttagtggtat gcttttttaa aaacaggagg gaaaaaatgg
    12421 atattcgtat actgacgtat tttttagcag tagctagaga aaaaaatatc agccgagcag
    12481 caaaatctct gcatatcacg caacctactc tctcaaaaca actgaaagat ttagaagaag
    12541 aacttggtgt gacacttttc actcggggct cccgagagat acagttgacg gattctggtc
    12601 gatatcttta ttcaaaagga aaagaaatct tgtcgttggt agaaaaaacg gcgaacaatt
    12661 taacaaatga cgcaatcatc tctggagaaa tctacattgg cggaggcgaa actaaagcaa
    12721 tctctttcat ttctgatagc cttcattcgt tgatcaaggc gcatcctgat atccagtttc
    12781 atttatatag cgggaatgca gatgatatca tagacaaact ggatagcgga ttacttgatt
    12841 ttggactcgt aatcgaaccg acaaacaaac aaaaatacca gtcatttcga ctgccagccc
    12901 aagatcgttg gggacttttg atacatcgaa atcatccttt agcaaagcaa accgtcatcc
    12961 atccgcagga actgaaaact gttcctttga tcatttcccg gcaaacgtca gtggacagtc
    13021 aactagctga atggctaggt ttcagcatag atcagttatc gatcgtcgga acatataact
    13081 tattgtacaa tgcttctttg atggtcgata acgggacttt tggtgcatta gctctagatg
    13141 ggatcatcaa tacttatggg acggaaaaag tattcatccc actcgatccg ccattacaag
    13201 cttcactgaa tctcatttgg aaaaaagacc agcctctttc tcctgctgct cgtaaatttt
    13261 tagatgtcct aacggagaaa tacagttaag tagagttttt ttccttcgct ttcagctttt
    13321 taacaacgaa taagttaagc aaaagatcca atgattaaat aacataagtt tcttaatttg
    13381 ttccttcctt ttatttctga taattccagt aaattactat aattataaaa aaagaattga
    13441 caaaacataa ctgtctatat aaaattgatt acgttaacaa taattcgttt catgtgacta
    13501 gatcatacta ggcatgaaaa atctctgagg aacatggttt ccctaggagg tttttcgtgt
    13561 ctatttttgt ttggccgcaa tcaactggtc tagcaaacgt gaaaaccaaa tcttcaaaaa
    13621 gggtgcgcgc atgaaaatca aaaagatctt taatcaaaat gcagtattgg tagatgacgg
    13681 tggacaggaa aaagtagcta ttggaaaagg gattggattt gacagaaaaa gaaatgattt
    13741 gatttttgat cgtgatgtcg aacggatatt tgtcatggaa ccagaaggtc aaatcaagtt
    13801 gcaaaattta cttaatcaga ttgatgaacg ctttttattt gcagcagaaa cgatcattga
    13861 tcatgcggaa actgttttga tggaaaagtt gaatgaacat gtattgatcg ctttgaccga
    13921 ccacattgct ttttctgcgg agaacattaa taacggtatc gtcatccgca acaaactttt
    13981 aagagaaatc gaagtgcttt acagtgatga attcagtatt gctcaatggg cagtagagta
    14041 tctgacaaaa gagcttggga ttccatatac ttatgacgaa gcaggatata tcgcaatcca
    14101 tattcacagc ggacgaagcg gtcggaacaa taaccatcgc agtatccgtg aagtgacgat
    14161 tgtatctgac atcatccatt tagtggaatc agaattggaa acagatatcc attcagaacc
    14221 attttcactg aattattcaa gactggtgaa tcatttacga ctgcttctac aacgaaccca
    14281 tgcgcaacaa tatgctgttt tagacacgga aatcgtagaa atggtcaaac gaaaataccc
    14341 agaaagctat aagatttcta aaaaaatccg tgtgttgctg accaaagaat accagctcgc
    14401 gatcacgaaa gaagaactgg gttacttggc catccatatc gagcgtttaa gaagcgcaat
    14461 cgtacaaaca aatgttaaca atcatgagga ggaaaaaaat tgaaagcata tctacaacga
    14521 atgggacgtt cccttcaact tccagttgcg gtcttacctg ctgctgcttt actagtaggg
    14581 atcggaaact ggtgggcatc ttatagtaac gacattgtcg ctcatttttt acaagctggc
    14641 ggtaacgcag ttttaggtca attacctttg ctgtttgccg ttgggttagc actagggatg
    14701 tcagtggaca aatcaggtgc tgctgccttg gcaggtttgg tcgcatttga agttccagta
    14761 aatgttttaa aaactgattc agtagctact ttattgaata tcaaaccaga atcagttgat
    14821 ccatcttttg ctcagatcag taacgtattt attggtatta ttgccggatt gattgcggca
    14881 gctctttata atcgtttcca cgaagcaaaa ttaccgatgg ctttatcttt ctttagcggc
    14941 aagcgtctag tgccaatctt ggctgccttt gtcatgttgc tgatcagtgc agtgctactg
    15001 ctcgtatggc ctccggttta ctctgcttta gtttcttttg ctaaattcat tttgagttta
    15061 ggctggggtg gtgcaggtct ttatggcttc ttcaaccgtt tactgatccc tacaggattg
    15121 catcaagcaa tgaactccgt gttctggttt gacgtagcag gaatcaatga tattggtaaa
    15181 tttttggctt ctgaaggaac aaaaggcgta acaggaatgt atcaagctgg atttttccca
    15241 atcatgatgt tcgggcttcc agcaggagca tatgctattt acagaaatgc acgtccagaa
    15301 aacaaagcaa aaacagcttc cttgatgtta gcagcagctt ttgcttcatt ctttactggt
    15361 gtaacagaac cacttgaatt ctcatttatg ttcgttgctt ggccattata tgtcttgcat
    15421 gcaattttca ctgggatttc tttagcagtg agtgcattct tccattggac agcaggattt
    15481 gcctttagtg ccggatttgt cgattacttc ctatctttga aaaatccgat tgcgaatcag
    15541 ccattgatgt tgatcgtcca aggattagta acagcagtga tttactactt tggctttaac
    15601 tttgctatca aaaaattcca tctgatgact cctggccgcg aggaagcaga tctttcagat
    15661 gacgatactg caacgactaa ttcagacaat aaatatgcgg cacaagcaag caaaatctac
    15721 gctgcattag gcggagccga caatgtaact tcgatcgata actgtactac acgccttcgt
    15781 ttgcaagtca aagatacagg cctgatcgat caaaataaaa tcaaagccac tggcgtacct
    15841 ggtatgaaga tcatcgacgg aaaaaatgcg caagtcatcg taggtacaga agtacaattc
    15901 gttgcagatg agatggcaaa actacatggt ggtgctgcaa cacgacctgc acaaacaaac
    15961 accgtggtta aaacagaaac atttgccggt ggaacgatcg aacaagatat ttacgctatc
    16021 gcaaatggga aactgattcc aatcactgaa gtttcagatg atgtattcgc ggaaaaaatg
    16081 atgggtgacg gctatgctgt attaccagaa aatggagaaa tcttctctcc aatcgctggt
    16141 acgatcacaa acattttccc aacgaaacat gctgtcggaa tccaaacaga tgctggtatc
    16201 gaagtcttgc ttcatatggg catcaacact gttgacttaa aaggcgaacc attcacttta
    16261 tatgtaaaag aaggtcaaaa agtcgcaaga ggtcaattga ttgctttagt tgatcttgcc
    16321 gctattcaat ctgcaggcaa aaacacggat atggtcgttg tattcacaaa tggcgacaaa
    16381 gtccaaagct tagaaatcga acccgcacgt gatgtaaaag caaatgataa aatcggctct
    16441 gtttccaaca aagcctgagc catcgtcttc cccagacttt tttatcgata aacaaacagg
    16501 agctgagaca aaaatcttgt ctcagctcct ttttccgaat aaaaacggcg ttctcctgcc
    16561 aactccgcgg taataagtcc agccaaacgt aacatatgaa gagctatttt ttatttgact
    16621 cttcttatta cttgatgcag aacagctttt ttacaacctc tgataaacgg tgttcaaaaa
    16681 ccagtttatc ggtatgaagt caaatttatg caaaaatgtg gaaagccatt ttcacaaaat
    16741 ttttcttcat actcaaaact aatcggcttt ttcacaacct ctttatacat tattcctgat
    16801 ccaatatttc ttttaatgct gcttcaagag aagttaatgg ttttcccaat actttttcta
    16861 aatcatcaga ggaaatagct aactgatcct ttttaatatc atgttggatc gctaaaaaca
    16921 tgtcagctgc ttcttgaggg aatccggctt cttttagatt ttcgacaaac gctgcatcac
    16981 ttgcttcaat aatttctaac tttttacctg tcgcacgctc taatgccttg gctaattctt
    17041 catagtcagt tagattgccg gataattcta agatttctgg agagtcactt tctactagta
    17101 cttttgctgc agcttctgca tattcacgtt tcaatgccca acctgttttt ccatttcctg
    17161 cagcataaac gaattgacca gtttttaaag cctgcccaat gataggtaat tcattctcta
    17221 cataccaatt attccgtaaa aatgtatggg aaatacctga atccttaatc attttttcag
    17281 tgaattggtg atctggcgct aatacactga tcgagtgatc ggcatctgca aaacttgtat
    17341 aagcgatata agatacccca gctgcttgtg ctgccttcac tacattctta tgttcggctt
    17401 gacgatttcc tggaacgcca gagacaaaaa gcaatcgatc tattcctttc aacgcttctt
    17461 tcattgagtc taaatcatca taatcaccaa tacgtatatt aaatccagct tctttcagtg
    17521 cttctccttt ttcttcacta cgtactagta catagatttc attttgtgga accatctttt
    17581 ttaagaattc cagcgcataa ccaccaaaac ctccagttgc tcctgttact aaataatgca
    17641 tatttgccac tccctattct ttttatttgt taagatttac agctgtacat ttataaatta
    17701 cacctttatc ataaagagtg tggtaagata agtcaagcaa aaaaaatgaa aggatgacaa
    17761 ctgatgaagt attctctgca atttagtgat gcaatccatg tacttgccta tattgaaatg
    17821 tttcaagata cccgatcact ttctagtgag atgattgcca gtagtatcga aacaaatgca
    17881 gcaaatgtac gtaaaatcat gagtcagctc aaaaaatccg gtttgatctt tacaaaaacc
    17941 ggaaaaccgc agccttccct tgccaaaagt ccagatcaga tttctttact agatgtctat
    18001 cgcagcatcg aaggtaatac acagcttatc caagtcgatc caaaaacaaa tccaaattgc
    18061 gtcgttggtg cgaatatcca agaagcttta gaagaaagct acgctcggct gcagaaaaag
    18121 gcagaagaag aaatggctaa gatcacactt gatagtctga tacataaaat agccgttttg
    18181 gaaaaagaaa aaagaccaga aaacacaggc ctcgtagaga gatttttata attctctcta
    18241 ctgtgcctgc atttttctga tcggaatcgt ctacataaat tcttttattc tccgggggac
    18301 aaaagccttg tcatgcccct ttttcctctc taatgctcag gtcaaagcgc tgaagtcatg
    18361 acgtcttgat aaacggtgtt caagtagctg accctcggca ttagttctga aaaatgcaaa
    18421 atatggaaag ctatttccca tttttcctct ctaatgctca ggtcaaagcg ctactttcac
    18481 gacctctata ttatcatggt ttattgacta ccggtatcca gatttctccc atatatttcc
    18541 cgttggtaac tcccatttcg actacagcat ttggaccccc aacataggcg taatcttcta
    18601 cttctcttaa aacatgccca aaggccgtat tcgtcaaatc atttcttaat tcttcaggcg
    18661 attctgctaa agcgattaca acaagatacg ttccttttgg gaatgggatg attcgggtag
    18721 tgtttggaac atcctgactc gtttgaacac cgacatagta catcattttc ccgttatagg
    18781 gttcgttgac tgtaaagaaa taatgatttt cagcagcttg tttcaatgca tctattgttc
    18841 cattaccgat aactttttgc tcaaatgctt gtttctctgc agccaaaccg acaaaatccg
    18901 tattgctact cttcaattcc acaccaatac ccgtcaccgt aaaatctggt ttttcttcga
    18961 tcctatactt tgccatttct cccacccttt ctattatatt aagttcatct tacataaaat
    19021 taaagggaaa tgacacgatt acgctctttt ttctagggat atttttattt tttaactatt
    19081 tttttgcttt cactgcaatc caaatctcac tgtaaacatt actgaaatcc tctccccatt
    19141 ttgtaaaaga gatctcggga gcttcgacta actcataatc tgaagaagga agccattctg
    19201 catagatttt cccccaagta tcttgtaaag tttggggaaa tggcccttta ttaggaaaaa
    19261 tggcccaagt gtgttcttct atagcaagca tttctaaatc agcgtacgga ttttcttttg
    19321 tcgtagcaaa gccaatcatg tgcatcaaac tgcccttttc ttccaagcgg gctccatcaa
    19381 atccataaga aacgttgatt acttgatttg gatataaatc tcctaactta tgcatctctt
    19441 ccctttgagt ctccgtgatc gtctgcgcta acttttgtat ttcttgattg actccttcaa
    19501 atttgacagg aacttttttc gccacacctg ccacataaaa ctgttctttc ttttcgatac
    19561 gaaattccat cgattttcct cctctgacat caataaaaaa tgtcaagcgt ggaaaagatt
    19621 tttgtatgcc gtatctgctg atttctgaag gcagatatcc tgaccaatca cgaaaagctc
    19681 ttgaaaaacc ttcgactgtt tcatatccat atctaaacgc cgtttctgtc actcttgatc
    19741 catctaggag atcgcgatta gccaaagcca atctacggtt tttgatatag tccattagcg
    19801 acatccctgc aataaacgta aagatgcgtt ttaattgata ttcagagatt cctgtatttt
    19861 cggctattat tttataggaa agtccttctg tcaaatgttc ttcgatgtaa tcgatcaatt
    19921 ggttcagatc tttaagcata tttatcttcc tccttgcttg cctttttatc atattatcaa
    19981 agaaagtcaa ttacttgatg aatcaggaac caatttagtc agatgtagaa aatgttgtat
    20041 ggatctaaag aaaattctca agcagcgttt cgtaacggat aattggtatt atctgcctat
    20101 ctatgtttta cctttagaaa aataaatttt ggggaagaca cattctaagt aaacaaagcg
    20161 gtaaaaatat cttttattcc cttagttaat ccgcttacta cctactgtac aaagcaaatt
    20221 gttgttcaaa ctgctgatca gaacgctgct tggatttttc tttgcgatac atcatacctc
    20281 ttttatcaat tggaacaatt atttatttct aacaaaatca cttcattttt gtcagataaa
    20341 taaaaaacaa aaaaagttcc atctttaaaa cacgctattc ttttactttt tctcacattc
    20401 tctaagaaaa gaaaaggaga atgaattttt gacaattcga gcaaaagtga ttatcaaata
    20461 tgtaaaatca gctacttttt tcttaaacgt ttctttatac taaatataaa taagtgtttc
    20521 atgaaaggag aactagatga ataattggct tgagtatttt cctgaaaacg ttttggaacg
    20581 cggttacagc tatcatctgc acggttttgt ccgtcatttg aactatacaa gcaagtactt
    20641 gtccgcaaca gtttctggca cggaagatta taaggtagtg atcacttggg atgaaaagac
    20701 caacatgact tgtgattgtc tatatgccat agaaggaaaa aaatgcaaac acatggctgc
    20761 tgtcttattc gcttacgaag aacgtccaat aaaaaaatcc aactactcac tttccgaact
    20821 ctcgtccctt gtttcctccg ctagttcctc tcttgtacgg gagcttttaa cagagattct
    20881 tatagaacat ccgcaattta tcgaacgctt caaagttaag atgccatttc atgcaataaa
    20941 ttattcagat aaattaacaa ctatcatcca taaatacgat catattatca agaaaaacaa
    21001 aaacaggaaa acagcgaagt tcatcatgga gatgcgaaag ttcatccaag aagcagttga
    21061 atcgctcatt cagcaaaatg cctatcttcc cgcattcgaa ttgatcaatg aagtgattgc
    21121 tactctcgaa acattctatt gggagcctga ggatgaacga acccttcttc tgatagagga
    21181 ctgttattat ttatggaaag aattattagc agaagcacct catgcagaaa aaaggcaaat
    21241 gttctcttgg ttcgtctgcc aagtcgatca tactgacgcc tcttattcaa aacgctactc
    21301 cataaaaatc ctgaaagaag actttcggga aaaagaattt tctaaccaaa agaaaaaaat
    21361 agataaaaaa actaaagaaa cggtaaagaa agacgatttc aatgaaaaat agcaagtaaa
    21421 cgataacaac catcctaaat ccacaagaaa tacagcagac cttgctttaa aaacacgaat
    21481 catgcaaagg atgaagtaaa gaagacttaa cgcgtaccca atattagtca tttgactatt
    21541 aggtacgcgt tttgttttat aagtcatgac aaatttcctt cgttgctggg tagattttcc
    21601 ggcattgttg atagatttta tgatactttt ctacattttg taagagaggt tccactgcat
    21661 ctgtatattg gataaaattc tgacaacaac tttcaggtgt gtcgaaccac tctaatcttt
    21721 aaagataaat attaaaataa cgattaataa tattaacagt ctttattttt caccaccaaa
    21781 aacctatctt tgcatatact aaatagacgt atatacacag ataggtttgt cttgtgaaaa
    21841 ataatgatta tccttaccaa aaaatgtttg tttttctaca tgttcgtact tatcaaaaaa
    21901 tatgatcctt attgaaatta ctggctattt atattccaac gacttcttgt gttaattttt
    21961 gttatctcat agtaaattat gtttcatttt cacttaattt agttgtcacg tgttcgtcaa
    22021 ttcgtaaaaa cggtaaatat ataaacacac ttgcggctat aattaatatt tgtaaaacag
    22081 ctgctcgcca gtctccggct gttgctaaaa atcccgatat aactggtggc gtagtccaag
    22141 gaatttgcac tactacatga ttaattaatc ccacagcagt tgcaaaatat gctactgtca
    22201 aagaaaaaat cggtgcaagc acaaagggaa taatcagact gatattaaat acaataggca
    22261 atccaaaaat aataggttcg cttatattga atatagcggg agttaccgat aatttagcaa
    22321 agtccttata atcttttcta cgactgaaga taaatattgc aatcaataaa gcaatcgtat
    22381 ttcccgatcc acccattacc gcaaaggtat ctttaaaggc catattgata atatgtggaa
    22441 tttgctcttt gtttgcataa gctgccatat tttcttgcat attctgaatc aaaaacggat
    22501 ctaacaatga acctgatatt actgcttgat ggataccaaa tccaaaaaat agattagcaa
    22561 tagatgtaat taacaaaaat cctggtaaac cagttgtaat tttacttaat ggctctgtaa
    22621 ctatcgtggt aattagctta tttatatcca tattaaacaa ttgaatgatt ataaatgaca
    22681 ataaagcaaa cgaggtaatc gtaataatga ttgggattaa tacattaaaa gactttaaaa
    22741 cagcgggagg aatatttccg gatagctgaa tctgtagttt cttattatta gaaagtttaa
    22801 taaataattc tgtagccgct aatccaacaa atatacctac aaacattcct gaagctcctg
    22861 tataggatat aggtatcaca cccgtcactt caacagcttt atccgcattt tcaggagtaa
    22921 acatcatctg cataggtgtt aatacaacaa atgtcgaaat agcaactaat accgctgcaa
    22981 tatgatttgt ataatttctg cttgcacaca tatgataaga tactgctgct acaactaata
    23041 atgtagttat gcttaatgta ccattggcaa ttgaagtacc aatttgttgt aacgtggtca
    23101 atgtactagt atcgacaatt tcccccatga atcctgttgg ttctaagatc actgaattaa
    23161 taagtgtcac aaatcctgct aacatcataa atggcataaa tgtagcaaac gcatctcgca
    23221 aagttcttaa atggatctga ttccccaact tagtcgcaac tataccaaat ttttcaaata
    23281 caatgtcctt cctgctactt ttgcccatat tttttctcct ttatgcttat tgtaatcgat
    23341 ta
//
LOCUS       DAZKLD010000031        22944 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.031, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000031 DAZKLD010000000
VERSION     DAZKLD010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22944)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22944)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22944
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.031"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            245..568
                     /gene="relB"
                     /locus_tag="ACG8KR_001003"
     CDS             245..568
                     /gene="relB"
                     /locus_tag="ACG8KR_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB family
                     antitoxin"
                     /protein_id="HFQ0200393.1"
                     /translation="MRFFKRGNYRAMITVRVSDAKKEWLSYMAEFYGITLSELLKNYS
                     MEQLEDEYNRQTAEIAHKHWIRSGKTTVFMEESLMNLVGWNDLPFKIHARNSEAALIG
                     KYVYW"
     gene            888..1115
                     /locus_tag="ACG8KR_001004"
     CDS             888..1115
                     /locus_tag="ACG8KR_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HFQ0200394.1"
                     /translation="MKIAFHIFADFDLMPKVSFAITVYREAVKAPCSSDDNKQAFLEL
                     GFLDSKYAAYFNELVLERDFKKVENRHDFST"
     gene            1131..2297
                     /gene="hemW"
                     /locus_tag="ACG8KR_001005"
     CDS             1131..2297
                     /gene="hemW"
                     /locus_tag="ACG8KR_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HFQ0200395.1"
                     /translation="MIETINPAENKVSAYIHIPFCEHICYYCDFNKVFLEGQPVDEYV
                     EMLLNEMRLMLESHPTANLETLYVGGGTPTSLSAKQLDRLLSGAREILPFQNGNEFTV
                     EANPGDLTREKLQVMKNYGVNRLSMGVQTFDNRLLKKIGRKHTAEDVYQTMQFLEAEN
                     FTNVSIDLIYALPGQTLEGNRDTLEKALALDLPHYSLYSLILENKTMFMNWVRQGRLE
                     LPDQETETRMFEETIEAMEKHGRHQYEISNFGLSGHESQHNLMYWNNDHYFGFGAGAS
                     GYLGDIRYRNKGPIQHYLRPLRANERPVLEEEKLTRKNQIEEEMFLGLRKKTGITKKH
                     FYDRYQKTIESVYGDVLRELEQEGLLVNEEDRVYLTPRGTFLGNNVFERFLFEK"
     gene            2532..3575
                     /gene="hrcA"
                     /locus_tag="ACG8KR_001006"
     CDS             2532..3575
                     /gene="hrcA"
                     /locus_tag="ACG8KR_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HFQ0200396.1"
                     /translation="MLTKRQNDILRLIIQHYTSSGVPVGSKTLMSEGVKASSATIRND
                     MKALEDEGLLLKTHSSSGRIPSALGYRYYVDHLLKPARVAEDDLLLIRQSLGREFHEI
                     NEIIKQSAEILSELTSYTTFSLGPEIKDRRLTGFRIVPLNNRQIIAIVVTDMGNVESQ
                     VFTLPEYMGSQELEKMVQIVNDRLVGDPLMTVYHKLRTEIPMILHKYFQTTEGMSQLF
                     DTVLGHVFEEKVYVSGQMNLLDYEPRQDLDQFKSMFSFMKDSDGLTQMITPMDSNIHI
                     KIGAELGNDLLQNMSMIQASYEIDGHGRGTIALLGPTSMPYSRMFGLVDVYRRELAAK
                     LADYYRSLDLSNS"
     gene            3669..4232
                     /gene="grpE"
                     /locus_tag="ACG8KR_001007"
     CDS             3669..4232
                     /gene="grpE"
                     /locus_tag="ACG8KR_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HFQ0200397.1"
                     /translation="MKENQEELDKEMTDAQPEPEIDVEAAEDSGISEAEAEEFETAKL
                     KAELEEMEDKYLRARAEIANMANRGKNEREQLQKYRSQDLAKKLLPSIDNLERALATE
                     VSDDQGAGLKKGVEMVLESLRNALEEEGIEKIPAKGEAFDPNLHQAVQTVPATEDTPA
                     DTIVEVLQEGYKLHDRVLRPTMVIVAQ"
     gene            4283..6112
                     /gene="dnaK"
                     /locus_tag="ACG8KR_001008"
     CDS             4283..6112
                     /gene="dnaK"
                     /locus_tag="ACG8KR_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HFQ0200398.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGNEAKIIANPEGNRTTPSVVSFKN
                     GEIQVGEVAKRQAVTNPNTISSIKRHIGEAGYKVDVEGKSYTPQEISAMILQYIKGFA
                     EDYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTD
                     RDEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNHLGGDDFDNKIIDYMVAEFKKE
                     NSIDLSQDKMALQRLKDAAEKAKKDLSGVSSTQISLPFITAGESGPLHLEMTLTRAKF
                     DELTADLVERTKVPVRQALKDAGLSQSEIDEVILVGGSTRIPAVVEAVRKETGKEPNK
                     SVNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTS
                     KSQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDID
                     KNGIVNVSAKDLGTQKEQKITIKSSSGLTDEEIERMVKDAEANAEADKARKEEVDLRN
                     DVDALLFSVDKTLKELEGKVDEEEVKKAETARDELKAAVEANNIEEMKTKRDALNEIV
                     QNLTVKLYEQAAQQQAQENPEAAQGGADDVVDADFEEVDGDDK"
     gene            6263..7429
                     /gene="dnaJ"
                     /locus_tag="ACG8KR_001009"
     CDS             6263..7429
                     /gene="dnaJ"
                     /locus_tag="ACG8KR_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HFQ0200399.1"
                     /translation="MATKRDYYEVLGLSKGASEDEIKKAYRKLSKKYHPDINKEPDAE
                     EKFKEVSEAYEILSDPQKRAAYDQYGHAGTDPNYGAGGAGGGFGGFGGFSGGGFGGFE
                     DIFDSFFGGGGRSVDPNAPRQGADLQYTVDLSFEEAIFGVEKEIKYNREEICHTCGGN
                     GAKPGTQPTTCHKCHGAGTINVERQTPLGRVMSRQTCDVCHGTGKEIKEPCPTCHGSG
                     HEKKAHSVKVNVPAGVEEGQQMRLAGQGEAGENGGPFGDLYVVFRVEESDIFDREGSE
                     IYYELPLSFVQAALGDEVQVPTVHGDVKLKIPAGTQTGTNFRLRGKGAPKLRGGATGD
                     QHVKVKLITPKNLNEQQREALRAFAEAGGIKVEEQQEDGFFDKFKDAFGGKKRK"
     gene            complement(7797..8534)
                     /locus_tag="ACG8KR_001010"
     CDS             complement(7797..8534)
                     /locus_tag="ACG8KR_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFQ0200400.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKDFLNKVLNI"
     gene            complement(8536..10038)
                     /locus_tag="ACG8KR_001011"
     CDS             complement(8536..10038)
                     /locus_tag="ACG8KR_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HFQ0200401.1"
                     /translation="MKRKHLFHSFVFLMTGLVTLAFGHVAQADNTTTAESSTSETMQK
                     IEPKKDVYVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAAL
                     QALESGQADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTV
                     GAKVGTESAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQG
                     QELETPIKKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSET
                     SSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRA
                     LRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIV
                     RGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISA
                     IGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
     gene            10297..12291
                     /gene="uvrB"
                     /locus_tag="ACG8KR_001012"
     CDS             10297..12291
                     /gene="uvrB"
                     /locus_tag="ACG8KR_001012"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HFQ0200402.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIFPASRDERALR
                     VEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHMEHAVANIKAELEQRL
                     TVLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGRKEGEPPYTLLDFF
                     PEDFLIVADESHVTMPQVRGMYNGDRARKQMLVDYGFRLPSALDNRPLRLEEFEKHVN
                     QIIYVSATPGPYEHEQTDTVIQQIIRPTGLLDPVIEVRPIMGQIDDLVGEINERVEKD
                     QRVFVTTLTKKMAEDLTDYFKELGIKVKYLHSDIKTLERTEIIRDLRLGEFDVLVGIN
                     LLREGLDVPEVSLVAILDADKEGFLRSERSLVQTIGRAARNEEGKVIMYADKVTDSMR
                     LAMDETSRRRTIQQKYNEEHGIVPKTIIKEIRDLISITKESEDDTKEAVQVSYEEMTK
                     EEKDTLLMKLEKEMKNAAKALDFETAANVRDMILELKASK"
     gene            12301..15117
                     /gene="uvrA"
                     /locus_tag="ACG8KR_001013"
     CDS             12301..15117
                     /gene="uvrA"
                     /locus_tag="ACG8KR_001013"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HFQ0200403.1"
                     /translation="MANDKITIHGARAHNLKNIDVTIPRDKFVVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEINDYLRLLFARVGHPICPNDHIEITSQSVDQMADQVMALPERTKIQVLAPIVVKK
                     KGQHKKIFERIQKEGYVRVRVDGEIYDLSEVPELEKNKKHDIAIVIDRIIVKEGIRSR
                     LFDSLEAALRLADGYALVDVIGEEEMLFSEHYACPYCGFTVGELEPRLFSFNAPFGAC
                     PDCDGLGVKLEVDQDLVIPDRTKTLSQGAIVPWNPISSQYYPQMLAQACESFGIDLDT
                     PFEELPEEHQDIILNGTDDLFHFHYENDFGGVRDVETTFEGVLKNIERRYHDTNSDFT
                     REQMRLYMTELTCQTCKGYRLNPQALSVQINGTNIGQTNELSIQKAVDFFSNLTLSEQ
                     EKVIAKPILKEVNDRLSFLENVGLDYLTLSRASGTLSGGEAQRIRLATQIGSNLSGVL
                     YILDEPSIGLHQRDNDRLIGSLKKMRDLGNTLVVVEHDEDTMWAADYLIDVGPGAGEQ
                     GGQIVAAGTPEEVANDENSLTGKYLSGKKSIPVPKERRKGNGKAIKITGASENNLKNI
                     SVEFPLGEFVAVTGVSGSGKSTLVNSILKKSLAQKLNKNSAKPGKFKTISGYESIEKI
                     IDIDQSPIGRTPRSNPATYTSVFDDIRGLFAQTNEAKMRGYKKGRFSFNVKGGRCEAC
                     RGDGIIKIEMHFLPDVYVPCEVCHGKRYNSETLEVHYKGKSIADILEMTVEDAVEFFK
                     HIPKIHRKLQTIVDVGLGYVTMGQPATTLSGGEAQRMKLASELHKISNGKNFYILDEP
                     TTGLHSDDIARLLHVLQRLVDAGNTVLVIEHNLDVIKTADYIIDLGPEGGEGGGTILA
                     TGTPEEIINVKESYTGHYLKKIMV"
     gene            15380..16645
                     /locus_tag="ACG8KR_001014"
     CDS             15380..16645
                     /locus_tag="ACG8KR_001014"
                     /EC_number="6.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aspartate ligase"
                     /protein_id="HFQ0200404.1"
                     /translation="MMNSIENEEFIPILLGSDMNVYGMARSFNEAYGKICQAYASDQL
                     APTRYSKIVNVEVIPGFDKDPVFIETMLRLAKERYSDKSKKYLLIACGDGYAELISQH
                     KQELSEYFICPYIDYSLFERLINKVSFYEVCEEYDLPYPKTLIVREEMLVNGHLEQEL
                     PFEFPVALKPANSVEYLSVQFEGRKKAFILETREEFDLILGRIYEAGYKSEMIVQDFI
                     PGDDSNMRVLNAYVDEDHQVRMMCLGHPLLEDPTPASIGNYVVIMPDYNEKIYQTIKA
                     FLEKIEYTGFANFDMKYDPRDGEYKLFEINLRQGRSSFFVTLNGLNLARFVTEDRVFN
                     KPFVETTYGTNQSDKARLWMGVPKKIFLEYARENEDKKLAEQMIKENRYGTTVFYEKD
                     RSIKRWLLMKYMFHNYIPRFKKYFHVKEG"
     gene            16649..17380
                     /locus_tag="ACG8KR_001015"
     CDS             16649..17380
                     /locus_tag="ACG8KR_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate racemase"
                     /protein_id="HFQ0200405.1"
                     /translation="MENFFSILGGMGTMATESFVRLINHRTKATKDQEYLNYVLFNHA
                     TVPDRTAYILDRSEENPMPFLLDDIEKQNLLQPNFIVLTCNTAHYFFEELQAATDIPI
                     LHMPREAANELVRQHTTGRVAILGTEGSMKAGIYEREVKNLGFETVIPDTVLQEKINY
                     LIYHEIKESDYLNQELYYEILEEAVERLNCEKVILGCTELSLMHEFAEDNHYPVIDAQ
                     SILADRTIERALAERSKALDTASEK"
     gene            17515..18399
                     /gene="rapZ"
                     /locus_tag="ACG8KR_001016"
     CDS             17515..18399
                     /gene="rapZ"
                     /locus_tag="ACG8KR_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HFQ0200406.1"
                     /translation="MTDNLQLVIITGMSGAGKTVAIQSFEDMGYFCIDNMPPRLIPKF
                     WELIKESGKVTKMALGVDLRSRSFFEEIQNMLIEIENTNFIDTSILFLDASDEELVAR
                     YKETRRAHPMAMDGLITEGIRKERAILDDLKAQASVIIDTTTLTPRQLREKINESFKS
                     KGDQGFRIEFVSFGFKYGLPIDSDIVMDVRFLPNPHYIPELRPLTGKDQPVYDYVMSF
                     DETQSFYHKFLDLLETVLPGYIKEGKSSLTVAIGCTGGQHRSVALTERLANALGKNYK
                     VNVTHRDKDKRKETVNRS"
     gene            18396..19394
                     /gene="yvcK"
                     /locus_tag="ACG8KR_001017"
     CDS             18396..19394
                     /gene="yvcK"
                     /locus_tag="ACG8KR_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HFQ0200407.1"
                     /translation="MKMKTYRIRKPKIVVVGGGTGLPVILKSLRNQGADITAVVTVAD
                     DGGSSGAIRESIAMAPPGDLRNVLVALSDMPQFYEDIFQYRFKKEDQFLANHTIGNLI
                     IAAVSEMRGSTYEAIQLLAKMMHVDGHVYPSSETPLTLHAVFKDGTTAVGESKIAIDR
                     KTIDRVYVCNTNDKSEAKAARKVVSAIMDADMVVLGPGSLFTSILPNLVIPEIGEAML
                     KTTAETVYICNIMTQKGETEHFTDADHIRVLHKHLNQPFIDTVLVNTEKVPEDYMDPE
                     IYDEYLVQVAHDFNGLRNEGCRVISTDFLELKDGGVFHDGDKVVEELFRLVFGSKY"
     gene            19428..20363
                     /gene="whiA"
                     /locus_tag="ACG8KR_001018"
     CDS             19428..20363
                     /gene="whiA"
                     /locus_tag="ACG8KR_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0043937 - regulation of sporulation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HFQ0200408.1"
                     /translation="MSFAADVKKELTGLEVHREHAKAELAALLRMNGSLGIVNQQFIL
                     NVQTENAAIARRIYSLLKDHYQVRSELLVRKKMKLKKNNVYIVRLKQGTKDVLMDLDI
                     MDGMMFNGHVSDEIMGNSQKMRSYLRGAFMASGSVNNPETSRYHLEIFSIYEQHNQDI
                     CDMLNYYGLHARTLERRNGYISYLKGAEKIADFLTLIGATNSMLRFEDVRIVRDMRNS
                     VNRLVNCETANLNKTIDAASKQIENIELIEAKVGLHALPEKLQEIAELRLQHPEVSLK
                     ELGEMIPSGAISKSGINHRIRKINEFAEKLREEAV"
     gene            20931..21587
                     /locus_tag="ACG8KR_001019"
     CDS             20931..21587
                     /locus_tag="ACG8KR_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0200409.1"
                     /translation="MRFFTTFTIIMIAVLFIFLDIAKRNTAFLLYRVLLRAGLITFIS
                     IVGFFLFTVIVFIWRTPAPPLPEITYGEFPFRLEYELNEELHVIEDTLIVEFDGFGMN
                     EGIGRYRRWTSRLASGEDLVLLLEVSDNKQIFYFPGPANYYMGDRLNGYNHTFPSASF
                     IERERGITRRDILHDKELLEQFGPLDQNTINEEELLNQYNIRLVNWEISEPIVNNFGD
                     "
     gene            21766..22331
                     /locus_tag="ACG8KR_001020"
                     /pseudo
     CDS             21766..22331
                     /locus_tag="ACG8KR_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294436.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         7604 a         3860 c         5007 g         6473 t
ORIGIN      
        1 gtaatatttt ttaaataacc tataggcttc ttcaaaagta catcctccag taaattctaa
       61 gtactggagg attctttttc tagtgttgct ttactcatga aaaagtagtt ggctacttca
      121 tcaaaatttc tgaacttttt taatgtataa taaagagctc gatcatttac tacaaggatg
      181 ctagctccta cattttcaca gttttttagc aagtaaattt ttctaaaatc acatcaggaa
      241 atgcatgaga ttctttaaga gaggcaatta tagggctatg attacggttc gtgtaagtga
      301 cgccaaaaaa gaatggcttt cttatatggc tgaattttat gggattactt tatctgaact
      361 tctaaaaaac tattcgatgg aacaactaga agatgaatac aatcgtcaaa cggctgaaat
      421 tgcccataaa cattggataa gaagtgggaa aacaacagta ttcatggaag aatccttaat
      481 gaatttggtg ggctggaatg acctgccgtt taaaattcac gcccgaaatt cagaagcagc
      541 tctgattggc aaatacgtgt attggtagag atagaagata atcaattcgt tgttatcgct
      601 attcaagtag ggcaccgaag aaatgtctat gattaagaga ctgtttattt ttacaaaaaa
      661 ataataattg gaatctagtt aaaactcttg aagtgagaga tttgactaga tttttttatg
      721 aataaaataa ggacaaaatt gtttaggaag aatcatgagt gtaaaaaaat catccaagaa
      781 gtcgattctt ggatttttta gtggatttgt tcgccaaata cgtttagtat gcgtatatga
      841 gaggttgtaa gcaaagcgtt gtggtctgag cacttaagga ggaatctgtg aaaatagctt
      901 ttcatatttt tgcagatttt gacttaatgc cgaaggtcag ctttgcgatt accgtttatc
      961 gagaggctgt aaaagcaccg tgtagctccg atgacaataa gcaagcattt ttagagctag
     1021 gtttccttga ttctaaatat gcggcttatt tcaatgagtt ggtgcttgaa cgcgatttta
     1081 agaaggtcga aaacaggcac gacttttcga cgtaaggaga aagaatttca atgatagaaa
     1141 caataaatcc cgcagaaaac aaggtttctg cgtacattca tatcccattt tgcgagcata
     1201 tctgctatta ctgtgacttt aataaagttt ttttagaagg gcagccagta gatgagtatg
     1261 tggaaatgct gctgaatgaa atgagattaa tgctggaaag tcatcctaca gcgaatttgg
     1321 aaacacttta tgtaggaggc ggaacaccaa cttctttatc tgctaaacaa cttgatcgac
     1381 tattatcagg tgctagagag atcctgcctt ttcaaaatgg taatgagttt actgtagaag
     1441 cgaatcctgg tgatttgacc cgtgaaaaac ttcaagtgat gaaaaactat ggcgtcaatc
     1501 gtttatccat gggagtgcag acatttgaca atcgcttatt gaaaaaaatt ggacgtaaac
     1561 atactgcaga agatgtgtat caaacaatgc aatttttaga agcagaaaat tttaccaatg
     1621 taagcatcga tttgatttat gcattaccgg gacaaacact agaaggaaat cgggatactt
     1681 tggaaaaagc tttggctttg gatctgcctc actattcttt gtactcattg atcctcgaaa
     1741 acaaaacaat gttcatgaac tgggtacgac aaggaagact cgaactgcct gatcaggaaa
     1801 cggaaaccag aatgtttgaa gagacgattg aagcaatgga aaagcatgga cgtcaccaat
     1861 atgaaataag taactttggg ctctccggac atgaatctca acataacctc atgtactgga
     1921 acaatgacca ctattttggg tttggagctg gtgctagcgg ttatcttgga gatattcgtt
     1981 accgcaacaa aggaccaatc caacactatt tacgcccatt acgagcaaat gaacgcccgg
     2041 tattggaaga agaaaaattg acacgaaaaa atcaaataga agaagaaatg ttcttaggac
     2101 taagaaaaaa aacagggatt actaaaaaac atttctatga tcgctatcaa aaaacaattg
     2161 aatcggtgta cggtgatgtg ctaagggaat tagagcaaga agggttactt gttaatgaag
     2221 aagaccgtgt ttatttgaca ccaagaggga cttttttggg gaacaatgtg tttgagcgct
     2281 ttttatttga aaaatagttt gaagaatatt tgttttttat cgaaacttga aaagaaaaag
     2341 tttagaaagt ttgacaatga ggtattgaag tattgatata actggattta catcaattat
     2401 ttagaattag cactcttgtg tttagagtgc taaaaaatgg agtctttctc ttgacaggag
     2461 aagggcttct tgctatattt ataagtgaat tagcacttag aatatatgag tgctaatgag
     2521 aggtgaggaa gatgttaaca aagcgacaaa acgacatttt acgtctgatc atccaacact
     2581 atacgagtag tggcgtgccc gttggttcca aaacattaat gtctgaggga gtcaaggcca
     2641 gctcagcaac gattcgcaat gatatgaaag cattagaaga tgaaggattg ctgttgaaga
     2701 ctcactcttc ttctgggcgg attccatctg ctttaggcta tcgttattac gtggatcatt
     2761 tgttaaaacc tgctcgcgta gcagaagatg atttgctgct gattcgtcag tctctaggca
     2821 gagaatttca tgaaatcaat gagattatta agcaatctgc tgagatatta tctgagctga
     2881 caagctatac gactttttca ttgggacctg aaataaaaga ccgccgactg actggctttc
     2941 gtatcgtacc cttgaataat cgacagatta ttgctatcgt cgtaacagat atggggaatg
     3001 tagaaagcca agtgttcact cttcccgaat atatgggaag tcaggaacta gaaaaaatgg
     3061 tacagattgt aaacgaccgt ctagtcggag atcctctgat gacggtgtac cataaacttc
     3121 gtacggagat tccgatgatt cttcataaat atttccagac gacagaaggg atgtctcagt
     3181 tatttgacac cgttcttggt catgtttttg aagaaaaggt ttatgtcagt ggacaaatga
     3241 atctcttgga ttatgagccc cgtcaagatc ttgatcagtt caaatcgatg ttttctttta
     3301 tgaaagactc agacggactt acacaaatga tcactccgat ggatagcaat attcatatca
     3361 agattggtgc tgaattaggc aatgatttat tgcagaatat gagtatgatt caagcaagtt
     3421 acgagataga tggtcatgga agaggtacaa tagccttgtt agggcctact agtatgcctt
     3481 attctcggat gttcggtcta gtcgatgttt atcgaagaga actagcagct aaacttgctg
     3541 attactaccg ctcgcttgac ttgtcaaact cgtaatgtga ataacgaagc tatttgcagc
     3601 tgatcgttct ctctttctta gtaagagcaa tcaaacagct gactgaataa tgaaaggaag
     3661 actgtatcgt gaaagaaaat caagaagaac tagataaaga aatgacagat gctcagccag
     3721 aaccggaaat cgatgtcgaa gctgcagaag attcgggtat ttcagaagct gaagcggaag
     3781 aattcgaaac agcgaaatta aaagctgagt tagaagaaat ggaagataaa tacttgcggg
     3841 ctcgtgctga aatcgcaaat atggcaaatc gaggaaaaaa tgagcgtgag caattacaaa
     3901 aataccgttc acaagattta gccaaaaaat tacttccatc aattgacaac ctggaacgtg
     3961 cattagccac agaagtgtca gatgatcaag gggcaggttt gaaaaaaggc gtggaaatgg
     4021 tcttggaaag tttgcgtaac gcactggaag aagaaggaat tgaaaaaatc cctgctaaag
     4081 gagaagcctt tgatcctaat cttcatcaag cagtacaaac tgttccagcg actgaagata
     4141 caccagccga tacgattgta gaagtactac aagaaggcta caaattacat gatcgcgtgt
     4201 taagaccaac tatggtcatt gttgcacaat aattgtgcgg atataaaact tacactttca
     4261 attaaagaag gagacgttaa ttatgagtaa aattatcggt attgacttag gtacgactaa
     4321 ctctgcagta gcagtattgg aaggaaacga agcaaaaatc atcgctaacc cagaaggaaa
     4381 ccgtacaaca ccttctgttg tatcattcaa aaatggagaa atccaagtag gggaagttgc
     4441 aaaacgtcaa gctgtgacaa acccaaatac gatttcatca atcaaacgcc atatcggtga
     4501 agcaggttac aaagtagatg tagaaggtaa atcatataca ccacaagaaa tctcagcaat
     4561 gatccttcaa tatatcaaag gattcgcaga agactattta ggtgaaaaag tcgaaaaagc
     4621 agttatcact gtgccagctt actttaatga tgcacaacgt caagcaacaa aagatgctgg
     4681 taagatcgct ggtctagaag tagaacgtat cgtcaatgaa ccaactgcag ctgctcttgc
     4741 ctatggttta gataaaacag atcgcgacga aaaaatctta gtattcgacc ttggtggcgg
     4801 tacttttgac gtatctatcc ttgaactagg agacggtgtc tttgatgtat tgtcaacagc
     4861 tggcgacaac catctaggtg gggacgactt tgataacaaa atcatcgact acatggtagc
     4921 agaattcaaa aaagaaaaca gcattgactt gtctcaagac aaaatggctt tacaacgctt
     4981 gaaagacgct gctgaaaaag cgaaaaaaga tttatctggc gtatcaagca cacaaatcag
     5041 cttgccattt atcacagcag gagaatctgg accattgcat ttggaaatga cattgacacg
     5101 tgcaaaattt gatgaattaa cagctgactt agtagaacgt acaaaagtac cagtacgtca
     5161 agcgttaaaa gatgcaggtc tgtcacaatc agaaatcgac gaagttattt tagtcggtgg
     5221 atctactcgt atccctgcag ttgtagaagc agtacgtaaa gaaacaggaa aagaaccaaa
     5281 caaatcagtt aaccctgatg aagtagttgc tatgggtgct gctatccaag gtggcgtgat
     5341 cactggtgat gtgaaagatg ttgttttact tgacgttaca ccattgtcat taggtatcga
     5401 aacaatgggt ggagtgttca ctaaattgat tgaccgcaat acaacgattc caacaagtaa
     5461 atcacaagta ttctcaactg cggcagataa tcaaccagca gtagacatcc atgtattaca
     5521 aggtgaacgc ccaatggctg ctgacaacaa aactctagga agattccaat tgacagatat
     5581 tccagcagct cctcgtggta ttccacaaat cgaagtaaca ttcgatattg ataaaaacgg
     5641 gattgtaaat gtatctgcga aagatctagg tacacaaaaa gaacaaaaaa tcacgatcaa
     5701 atcttcttca ggtctaacag acgaagaaat cgaacgtatg gtgaaagatg cagaagcaaa
     5761 cgctgaagca gataaagctc gtaaagagga agttgaccta cgtaatgatg tagacgcatt
     5821 gctattctct gtggacaaaa cattgaaaga attagaaggc aaagtggacg aagaagaagt
     5881 gaaaaaagca gaaacagctc gtgacgaatt aaaagccgct gttgaagcaa acaacatcga
     5941 ggaaatgaaa actaaacgcg atgcattgaa cgaaatcgtt caaaacttga ctgttaaact
     6001 atacgaacaa gctgcacaac aacaagctca agaaaatcca gaagcagctc aaggcggagc
     6061 agatgatgtc gttgatgctg attttgaaga agtcgatggc gacgataaat aatcagttca
     6121 ctgaatttcg agagaaggcc aaagttgcta ctttggcttt ttcttttgaa aaatagtttg
     6181 aagtatcggt ttttgtttca aacaaaatat gatatgataa caagcgacgt gaaaatgatg
     6241 agaaccaata ggaggaaagc ctatggcaac aaaacgtgat tattatgaag tcttagggct
     6301 ctcaaaagga gcatcagaag atgaaataaa gaaagcttat cgtaagcttt cgaaaaaata
     6361 ccatcctgac atcaataaag aacctgatgc ggaagaaaaa ttcaaagaag tatcagaagc
     6421 atatgagatt ttaagtgacc cgcaaaaacg cgctgcatac gaccaatacg ggcatgcagg
     6481 cacagatcca aattacggtg ctggtggagc tggtggcgga tttggtggat tcggcggctt
     6541 ctctggtggc ggcttcggtg gttttgaaga tatcttcgac tctttctttg gaggaggcgg
     6601 tcggtcagtc gatccaaatg cgcctcgtca aggtgctgat ttgcaatata cggtcgactt
     6661 gtcttttgaa gaagcgattt ttggtgtaga aaaagaaatc aagtataatc gggaagaaat
     6721 ctgtcatact tgtggtggaa acggtgcaaa accaggcaca caaccaacta cttgtcataa
     6781 atgtcatggt gcaggtacga tcaatgtaga acgccaaacg ccacttggac gagtgatgag
     6841 ccgtcaaact tgtgatgttt gtcatggcac agggaaagaa atcaaagaac cttgtccaac
     6901 ctgtcatggc tcaggacatg agaagaaagc acattcagtt aaagtgaatg taccagctgg
     6961 tgtagaagaa ggacaacaaa tgcgtttagc tggtcaagga gaagctggag aaaacggcgg
     7021 accatttggt gatttgtatg tggtttttcg tgtagaagaa agtgatatct ttgatcgtga
     7081 aggttctgaa atttactatg aattgccttt aagttttgta caagcagctt tgggggatga
     7141 agtccaagta ccaactgtcc atggagatgt gaaattgaag atccctgctg gaacacagac
     7201 aggtacgaac ttccgtttac gtggaaaagg cgctccgaag ttacgtggtg gtgcaacagg
     7261 cgaccaacat gtcaaagtaa aactgatcac accgaaaaat ctaaatgaac aacaaagaga
     7321 agcattacgt gcattcgctg aagctggcgg catcaaagtt gaagaacagc aagaagacgg
     7381 tttcttcgat aagtttaaag atgcttttgg cgggaaaaag cggaagtagt ttttctagta
     7441 aaaatataaa gtagacttgc ccaaattttc tatttctagt aaaaacgcta agtcattaat
     7501 aattaaaaac cagacgaaca atcatacgtt cgcctggttt ttttgtagtt gtcatgtttg
     7561 taccaacaaa aaagccagct ttagaataag ataatcatca tagtttctga aggtggtatc
     7621 gactatgtac ttgatatcgt caatgaaccg atggtacttt cttatgcgat ggaacaggta
     7681 gagcgattgc ttgtttgtgc aggagaacaa gcgatgcggc tggtacagtt aagtgaaaaa
     7741 acaagaaact aaaaatgccc ggtcgtcatg aagcgaccgg gcatttttgg tttttattaa
     7801 atatttaata ctttattcaa gaaatctttt gtccgttcat tttgcggatt ttcaaagatt
     7861 tgttgaggcg ttccgtcttc taaaaagttt ccgtcgtcaa tgaacatgac acgattggcc
     7921 acttctttag cgaagcccat ttcgtgcgta acgataacca tcgtcatacc ttgttctgct
     7981 aactttttca tgacgttcaa gacatcaccg accatttcgg ggtcgagtgc agatgtaggc
     8041 tcatcaaaaa gcatgatgtc agggttcatc gctagagcac gagcaatagc cacacgctgc
     8101 ttttgtccac cggaaagcat ttcaggatac atatcttttt tatcttctag accaactgtc
     8161 tgcagcaatt tgattgcttt tttctctgct tcttctctag attctttctt caggtccatc
     8221 ggtgctaaaa tgatgttttc caaaatagaa agatgaggga ataggttaaa gtgctggaat
     8281 accattccga tatgttgtct caccaagttg atatctgtgc ttttatccat caagtgggcg
     8341 ccgtcaataa taatttcacc gtcagaaggt tcttctaaac gattcaagca acgcaaaaat
     8401 gtactttttc ctgaacctga agggccaatg atacagacga catctccttc gttgatggac
     8461 acattgatgt cgtttaatac tgtgttatcg ccgtattttt tgactaaatg ctcaactaag
     8521 attttttctg ccatcttact tcaccttctt ttctaaaact tttgctaatt ttgtcaatgc
     8581 tgtgatcaaa atcaagtaca tgatcgcaat gatcaagtaa acatacgtac tttgtgttgt
     8641 acgtgcaaca atgatcttac cagtttgcag caattcgact acaccgattg cggaaatgat
     8701 cgttgtgtct tttaatgaga taacgaactg attgacgaat gatgggatca tgatgcgtat
     8761 cgcttgcggc aaaatgattt tttgcattgt ccgattataa gaaagaccta aacttctgga
     8821 agcttccatt tgtccgacag gaacggcgtt gatcccgcct cgaacaatct ctgcgatata
     8881 agcacttgcg ttaagagtca aggtgataac accagcaagg aagtcaggaa tcgtaaatcc
     8941 aacgatacca ggcaacccaa agaaaataaa gaacgcaaga accatcattg gaattcctcg
     9001 gatgatatcg acataaatac tagcaattgt gcgaagagcg cgtattggtg ctacactgaa
     9061 taatccaaaa atcacaccga ttactatagc caaagcaaag gagatcaaag ttaggagaat
     9121 tgttctccaa agtcctttca acaatgcttg atagttgttt tggataagtc caagaaatgt
     9181 tgattcatca gcagtggaag actcactgga agtttctgaa ccatccgcta catattcttt
     9241 aataatctta tcgtattcgc ctgttctttt taattctttc aatccttcat tgaacatatt
     9301 aagaagctca ggattttgtc cttttttaac ggcaaatgca tattttccgc cagattcttt
     9361 tttgatcggt gtttccaatt cttgtccttg tttgatcgca taaccgatta ccggataatc
     9421 atccatcatt gcatcgattg cgccaacttt taacccgtcg tacaattgat cagcagcatc
     9481 aaaatatttg atagaatagc catattcaga ttcatggtcg cttaaaaatt cagcactttc
     9541 ggtaccaact tttgctccga ctgtttttcc tttcaaatct tcataagact tgatcgaagt
     9601 atctcctttt ttgacagcta attggatccc gctatcaaag taagaatcag aaaaatcata
     9661 gcttttcttt cgttcatctg ttacagtcat tccggcaatc attccgtctg cttgtcctga
     9721 ttccaaagct tgcaaagcag cagagaaacc acggtagtcc attcggatgt taaatccttg
     9781 cagtttggcg atgttttcca tcaattttac atcgatgcct tcgtactcgc cctttgcgtt
     9841 ttgaaattca aatggagcaa aagcagagtc actggcaatt acgtatacgt cttttttggg
     9901 ctctattttt tgcattgtct cgcttgtgct tgactctgcc gttgtagtgt tgtcagcttg
     9961 tgcaacatgt ccaaacgcaa gtgtcacaag tcctgtcatt aagaaaacaa atgagtgaaa
    10021 taaatgcttt cttttcataa tcaacctcct aaatttccaa tattttatta ttataggcga
    10081 aaagtgctga atcagacaag actgtaagct cgaaaaaatg aaaaaaatgc gagtttctaa
    10141 cattttatgt tataaaacgt aacatgcata ataagataaa aaagtggcga aagtatgttc
    10201 ggtgtggtat aattttttag taaggaatgc ctttgctttc cttttctttt tattggtcgt
    10261 aaaatgaaaa ggaatgaatg aaaaggagga cgaagaatga ttgagagaga tacctcccga
    10321 cactttgagc ttgtctcgaa ataccaaccg gctggtgatc agcctgaagc gattaaccaa
    10381 ttagtggatg gcgtagtcgg cgggaaaaaa gcacaaatct tattaggtgc gactggaaca
    10441 gggaaaacat atacgatttc caatcttatt gagaaagtga ataaaccaac actaattatt
    10501 gcccataaca aaacattggc tggtcagctg tacggggaat tcaaggaatt tttccctaac
    10561 aatgctgttg aatattttgt ttcttattat gattattatc agcctgaagc ttatgtgcct
    10621 tcaagcgata cgtatatcga aaaagattca agtgtaaatg atgagatcga caaattacga
    10681 cattcggcta ctagttcatt attagaaaga aatgatgtga tcgtcattgc atccgtttca
    10741 tgtatttttg gattaggttc gccttttgag taccaaaaac aagtagtttc gattcgacag
    10801 ggagcagaac tagatcgcaa tcagttgatt cgtgacttag tcagtatcca attcgaaaga
    10861 aatgatatag attttcagcg cggtcgtttt cgagtgcgcg gtgatgtcgt ggagattttt
    10921 ccggcttcaa gagatgaacg tgccttacgt gtggagtttt ttggagatga ggtcgaacgt
    10981 atccgagaag tcaatgcact gacaggtgaa gttttaggag aaacggaaca cgtggcaatc
    11041 ttcccggcga ctcactttgt gacgaatgat gaacatatgg agcatgccgt agcgaatatc
    11101 aaagcggaat tagaacagcg tttaactgtc ttgcgaaatg aaaacaaact tctggaagct
    11161 caacgtttag aacagcgaac caattacgat attgaaatga tgttagaaat gggctacacg
    11221 tcgggaattg aaaattattc tcggcatatg gacggacgta aagaaggtga accgccttat
    11281 acgctattgg atttctttcc tgaagatttt ttgatcgtgg cggacgaatc gcacgtaacc
    11341 atgcctcaag tcagaggaat gtataatggt gaccgagcga gaaaacagat gctagtagat
    11401 tacggtttcc gtttgccatc tgctttagac aatcggccac ttcggttaga agagtttgaa
    11461 aaacatgtca atcagatcat ttatgtctct gcgacgccag gaccatatga acatgaacaa
    11521 acagataccg ttatccaaca aatcatccgt ccgacagggc tgcttgaccc agtgatcgaa
    11581 gttcgtccaa tcatgggaca aatcgatgat ctagtaggcg aaataaatga acgagtcgaa
    11641 aaagaccagc gggtgtttgt aacgacattg acgaaaaaaa tggcagaaga cctcacagat
    11701 tatttcaaag aattagggat caaagtcaaa tatttgcata gtgatatcaa aacgctggaa
    11761 cgaacagaaa tcattcgaga tcttcgatta ggtgaatttg atgtgttagt tggaatcaat
    11821 ttgcttcgtg aaggattaga cgtacctgaa gtttcccttg ttgctatctt ggatgcggat
    11881 aaagaaggct ttttacgtag tgaacgttcc ttagtccaaa cgattggacg agctgctcgg
    11941 aatgaagaag gtaaagtaat catgtacgca gacaaagtaa cagattctat gcgtctagcg
    12001 atggatgaaa catcaagacg ccgtacgatt cagcagaagt acaatgaaga acatgggatc
    12061 gttccaaaaa cgatcattaa agaaatccga gatttgattt ctattacgaa agaatcggaa
    12121 gatgatacaa aagaagcggt acaagtctcg tatgaagaaa tgacgaaaga agaaaaagat
    12181 acgttattga tgaaattaga aaaagaaatg aaaaatgcgg cgaaagcatt ggattttgaa
    12241 acagccgcta atgtgcgaga tatgatttta gaattaaaag cttcaaagta ggaggacaac
    12301 atggctaacg ataaaattac gattcacggt gcacgtgccc ataacttgaa aaatatagat
    12361 gtaacgattc cacgagataa attcgtggtc gttacaggtt tgtccggttc tggaaaaagt
    12421 tccctagcat ttgatacgct gtacgctgaa gggcagcgga gatacgtaga gagcctttct
    12481 gcttatgctc gtcagttttt aggacaaatg gataagccgg acgtagatag catcgacgga
    12541 ttgagtccgg cgatttctat tgatcagaaa acaacaagta aaaatcctcg gtcgacagta
    12601 ggtactgtga cggaaatcaa tgactattta cgcttgttgt ttgcacgtgt aggacatccg
    12661 atttgtccca acgatcatat cgagattacc agtcaatcag tagatcaaat ggcagatcaa
    12721 gtaatggctt tgcctgagcg cacaaagatc caagtgttgg caccgatcgt ggtaaagaaa
    12781 aaaggacaac ataaaaaaat attcgaacga atccaaaaag agggatacgt ccgcgttcga
    12841 gtcgatggtg agatctatga tttaagtgaa gtgcctgagc tggagaaaaa caaaaaacat
    12901 gatatcgcga tcgtcatcga ccggatcatt gtgaaagaag ggattcgttc tcgtctattt
    12961 gattctttag aggcggctct gcgattggca gacggctatg ctctggttga cgtgatcggt
    13021 gaagaagaaa tgttgtttag cgaacactat gcctgtcctt attgcggatt tactgttggc
    13081 gaactagaac caagactgtt ctcattcaat gcgccttttg gagcttgtcc agactgtgat
    13141 ggtttaggtg tcaaattaga agtcgatcaa gatttagtga ttccagatag aacgaagaca
    13201 cttagtcaag gtgctatcgt tccatggaat ccaatcagct ctcagtatta tcctcaaatg
    13261 ttagcgcagg cttgtgaaag tttcggtatc gatttagata caccattcga agaattaccg
    13321 gaagagcatc aggatatcat cttgaatggg acagatgatc ttttccattt ccattatgaa
    13381 aatgattttg gtggcgttcg cgatgtagaa acgactttcg aaggggtatt gaagaatatc
    13441 gagcgtcgtt atcatgatac gaacagtgat ttcacgcgtg agcaaatgcg tctttatatg
    13501 acagaattaa cttgtcagac atgtaaaggt tatcgattga atccccaagc tctatctgtt
    13561 caaatcaatg gaacgaatat cgggcaaaca aatgagttgt ctattcaaaa agcagtagat
    13621 ttcttctcaa acttaacgtt atctgaacaa gagaaagtaa ttgccaaacc gattttaaaa
    13681 gaagtaaatg acagactttc attcttggaa aatgtgggac tagactatct aactttgagt
    13741 cgcgcttccg gaacattatc tgggggagaa gcgcagcgaa tcagattggc tacacaaatt
    13801 ggatcgaatc tttctggagt tttgtatatt ttagatgaac catcgatcgg attgcatcaa
    13861 cgcgacaacg atcgactgat tggctcatta aagaaaatgc gtgatcttgg caacaccctc
    13921 gttgtagtcg aacatgatga agatacgatg tgggcagcag attatctgat tgacgtcgga
    13981 cctggggccg gagaacaagg cggacaaatc gtggctgcag gcactcctga agaagtagct
    14041 aatgatgaaa attctttgac agggaaatat ctttcaggga aaaaatcaat ccctgtacca
    14101 aaagaacgcc gtaaaggtaa tgggaaagca atcaagatca ctggtgcttc agaaaacaat
    14161 ctgaaaaata ttagtgttga atttccatta ggagaatttg tagcagtcac aggtgtgtct
    14221 ggttctggaa agagtacatt ggtcaatagt atcttaaaga aatcgttagc gcaaaaatta
    14281 aataagaatt ctgctaagcc aggtaaattc aagacaattt ccggctacga aagtatcgaa
    14341 aagatcatcg atatcgatca aagcccaatc ggccggacgc cgagaagtaa tccagcgact
    14401 tatacaagtg tatttgatga tatccgtggg ttatttgctc aaacgaacga ggcaaaaatg
    14461 cggggttata agaaagggcg ttttagtttc aacgtaaaag gcggtcgttg tgaagcttgt
    14521 cgcggggatg gaattattaa gatcgaaatg cactttttgc ctgatgtcta tgttccttgt
    14581 gaagtatgtc atggcaaacg atataactct gaaacattag aagtgcatta caaaggaaaa
    14641 agcattgctg atattttgga aatgacagta gaagatgctg tagaattctt caagcacatt
    14701 ccaaagattc atcgcaaact gcaaacgatt gttgatgttg gcttaggtta tgtgacgatg
    14761 gggcaaccag caacgacatt gtccggtggt gaggcacaac ggatgaaact tgccagtgaa
    14821 ttgcacaaaa tctctaatgg aaagaatttc tatatactag atgaaccaac gacaggactt
    14881 catagcgatg acatcgcccg cttgttgcat gtattacaaa gattagtaga tgctggtaac
    14941 acagttttag tgattgaaca caatctagat gtaatcaaaa cagcagatta tatcattgat
    15001 ttaggaccag aaggtggaga aggtggagga acgatccttg cgactggaac accagaagaa
    15061 atcattaacg taaaagaaag ttatacaggt cactatttga aaaaaataat ggtataaaga
    15121 ggatagatag ctgagatagt taaaaaaaca taaaaaaatg aatttttgct tgttttatta
    15181 atatcagtgt tctagaatca ttacatggta gttctttcta cactgattgg tatagacaaa
    15241 aacttcctat aagttcaatc cgttcaaaaa agaattgagc aatcggagga gtttcctttg
    15301 attgcttttt tctttatggt atgatattcc ttgtgtagtt ggaaaagaaa aacaagagat
    15361 tatgagtaag tgagagataa tgatgaacag tattgaaaat gaagaattta tccctatatt
    15421 attaggaagc gacatgaacg tttatggtat ggcacgttct tttaatgaag catacggcaa
    15481 aatctgccaa gcatatgctt cggatcagtt agcccctacc cgctacagta aaattgtcaa
    15541 tgtagaagtt attccaggat ttgataaaga ccctgtgttt atcgagacga tgcttcgttt
    15601 ggcaaaagaa agatattctg ataagagtaa aaaatatctt ttgattgctt gtggtgatgg
    15661 atatgcagaa ttgatctccc aacataaaca agagctgtca gaatatttta tctgtccata
    15721 tattgattat tctttatttg aaagactgat caataaagta agcttttatg aagtttgtga
    15781 agaatatgat cttccgtatc ctaaaacatt gatcgtcaga gaagaaatgt tagtaaatgg
    15841 acatttggaa caggaattgc cttttgaatt cccagttgct ttgaaacctg caaacagtgt
    15901 ggagtatctt tctgtccaat ttgaaggaag aaaaaaagct tttattttag agactagaga
    15961 agagttcgat ttaattttag gccggatata cgaagccggt tataaaagtg aaatgattgt
    16021 acaagatttt atcccgggtg atgatagtaa tatgcgtgtg ctaaatgcgt atgtagatga
    16081 agatcatcaa gttcggatga tgtgtcttgg tcatccactg ttagaagatc ctacaccagc
    16141 atcgattggt aattacgttg tgatcatgcc agattataac gaaaaaatct atcaaaccat
    16201 taaggcattt ttggaaaaaa tcgaatatac aggctttgcc aatttcgata tgaagtatga
    16261 tcctagagat ggagaatata aattatttga aatcaatttg agacaaggtc gtagcagttt
    16321 ctttgtgaca ttgaacggct tgaatctggc tcgtttcgtc acagaagatc gagtgtttaa
    16381 caaacccttt gttgaaacga cctatggaac gaatcaatca gataaagcaa gattatggat
    16441 gggtgtgccc aaaaaaatct ttttagaata tgctcgggaa aatgaagaca aaaaactagc
    16501 tgagcaaatg atcaaagaaa atcgttacgg tactactgtg ttttatgaaa aagaccggtc
    16561 aatcaagcgc tggttgttga tgaaatatat gttccataac tatattccaa gattcaaaaa
    16621 gtattttcat gtgaaagaag gctagttcat ggagaatttt ttcagtattt taggcggaat
    16681 gggcacgatg gcgacagaaa gttttgttcg cttgatcaac caccgaacga aagctacaaa
    16741 agatcaagaa tatttgaatt atgtcttatt caatcatgca acagttcctg atcgtacagc
    16801 ttatatttta gatcgatcag aagaaaatcc aatgccgttt ctgctggatg atattgagaa
    16861 acaaaattta ttgcagccga acttcattgt gttgacttgt aacaccgcac actacttttt
    16921 tgaagaatta caagcggcaa cggatattcc tatccttcat atgccaagag aagcagcaaa
    16981 tgaacttgtc cgtcagcata cgacagggag agtagcgatt ctaggaacag aaggaagtat
    17041 gaaagctgga atctatgaac gcgaagtcaa aaatcttgga ttcgaaacag tgattcctga
    17101 tactgttctt caagaaaaaa tcaattatct gatctatcat gagatcaagg aatcggatta
    17161 tttgaatcaa gagctatatt acgaaatctt agaagaagcg gtagagcgcc taaactgtga
    17221 aaaagttatc ttaggttgca cagaattgtc attgatgcat gaatttgctg aagataacca
    17281 ttatccagtg atcgatgccc aatctatttt agcagatcgg acgattgaac gagcgctagc
    17341 tgaaagaagc aaggcattgg atacagcatc ggaaaagtaa aaaaattgga tttggggcaa
    17401 atatcctaaa agatatttgg tccaaatctt tttgtaaaag aaaaagaaaa ctggtaatga
    17461 tacgcccgga atgatataat tttactatgc acaatgtgag aggagttcgt aattatgaca
    17521 gataatttgc aattagtcat cataacaggt atgagcggtg cagggaaaac agtagctatc
    17581 caaagttttg aagatatggg ctatttttgt atcgataata tgccgcctcg attgattcca
    17641 aaattctggg aattaatcaa agaatcaggc aaagtcacaa aaatggcttt aggagtggat
    17701 ttacgttctc gttccttctt tgaagaaatc caaaacatgc tgatcgaaat cgaaaatacg
    17761 aacttcatcg acacttctat cttattttta gatgcttcag acgaagaact agtggctcgc
    17821 tacaaagaaa caagacgagc acatccaatg gcgatggacg gtttgatcac tgaagggata
    17881 cgtaaagaac gggcgatact agacgacttg aaagcccaag cttccgtgat cattgatacg
    17941 acgacattga caccacgaca attacgtgaa aaaatcaatg agtcattcaa atcaaaggga
    18001 gaccaaggat ttcggatcga atttgtatca tttggtttta aatacgggtt gccgatcgat
    18061 tcggatattg tgatggatgt gcgttttttg cccaaccctc attatatccc ggaactaaga
    18121 ccattgaccg gaaaagatca gccagtctat gattatgtga tgtcctttga tgaaacgcaa
    18181 tcattctacc ataaattttt ggatcttctg gaaactgtat tgcctggata cattaaagaa
    18241 ggaaaaagca gcttgacagt tgcaatcggc tgtacaggcg gtcaacaccg ttctgtggca
    18301 ttgactgaac gtttggcaaa tgctttaggg aaaaactata aagtcaatgt gacacatcgg
    18361 gacaaggata aaaggaaaga gacggtgaac cggtcgtgaa aatgaaaacc taccggataa
    18421 gaaaaccaaa gatcgtagtg gtcggcggtg gcactggtct gccagttatc ctgaaaagtt
    18481 tgcgaaatca aggagcagat atcacagcag tagtgactgt tgcagatgat gggggaagca
    18541 gcggtgcaat cagagaatcg atcgctatgg ctcctcctgg tgacttgaga aatgtcttag
    18601 tggcgttatc ggatatgcca caattctatg aagatatttt ccaataccgt ttcaaaaagg
    18661 aagatcagtt tttagccaat catacgatcg gtaatctgat cattgccgca gtttccgaga
    18721 tgcggggaag cacttatgag gcgattcaat tattagcaaa aatgatgcat gtagatggac
    18781 atgtttatcc ttcttcagaa actcctttga ccttgcatgc tgtctttaaa gacggaacga
    18841 ctgcagtagg agaatctaaa atcgcgatcg accgtaaaac cattgaccgt gtgtatgtct
    18901 gcaacacaaa tgataaaagt gaagccaaag cggctcgaaa agtcgtttca gcaattatgg
    18961 acgccgacat ggtcgttttg ggacccggaa gtttattcac cagtatcctt ccaaatttag
    19021 tgataccaga aatcggcgaa gcaatgctca agacaactgc tgaaaccgtc tatatctgta
    19081 atatcatgac ccaaaaaggg gaaacggaac atttcacaga tgccgatcat atacgtgttt
    19141 tacacaagca tttgaatcag ccttttatcg atacagtatt ggtcaatacg gaaaaagtgc
    19201 ctgaagatta tatggaccca gagatctatg atgagtatct agtacaagtc gcacacgact
    19261 tcaacggatt gagaaatgaa ggatgtcgag tgatttccac cgactttttg gaattgaaag
    19321 atggtggtgt cttccacgat ggagacaaag tagtagaaga attgttccgt ttagttttcg
    19381 gttcaaaata ttaagcaatt tattgttgga gaaaggagga gaataggatg tcatttgctg
    19441 cagatgtgaa gaaagaacta actggtttgg aagttcatcg agaacatgcg aaagcggaat
    19501 tagctgcttt gctgcgaatg aacggctctt taggtattgt gaaccagcag tttattttga
    19561 acgtccaaac agaaaatgct gcaattgcca gacgtatcta ttctcttttg aaagatcatt
    19621 atcaggtacg aagtgagcta ctcgttcgta aaaaaatgaa attaaaaaag aacaatgtct
    19681 atatcgtccg gttgaagcaa ggcacaaaag atgtgttgat ggatcttgat atcatggatg
    19741 gtatgatgtt caatggacac gtttcggacg agattatggg aaattcgcaa aaaatgcgtt
    19801 cttacttacg tggtgctttt atggcctcag gttcagtcaa taatccggaa acgagccgtt
    19861 atcatttgga aatattttct atttatgaac agcataacca agacatttgc gatatgttaa
    19921 attattacgg tctacatgct cgaacgcttg aacggcgcaa tggctatatt tcttatttaa
    19981 aaggagcaga aaaaattgct gatttcttaa cattgattgg tgcaaccaac tcaatgctcc
    20041 gatttgaaga tgttcgaatc gtacgggata tgagaaattc ggtcaatcgt ctagtcaatt
    20101 gtgagacagc aaatctgaac aagacgattg atgcggcttc taaacaaatc gaaaacatcg
    20161 aattgatcga agcaaaggtc ggtctacatg cccttcctga aaaacttcag gaaatcgcag
    20221 aacttcggct tcaacatcca gaggtcagct tgaaagaatt aggggaaatg attccctcag
    20281 gtgcgatatc aaaatcggga attaatcacc gaattcgcaa aataaatgaa tttgcggaaa
    20341 aactgcgtga agaagcagtt taatccaaag aaaaaactga tacatgaagc aggagatgcg
    20401 aatgtatcag ttttttttaa aagttatact tgcttttctt ccgtcaaact ataattccat
    20461 ctttactgga gtaacataat ttggaaaggc ttctattgtt tttaatacaa tcgtataaaa
    20521 agcggggaga caactgaatg aagattttag ttttgtacat aaatttttgc ttaatataga
    20581 agtcctgata tagataagat caagtattta tctataactg gcctgattaa ttaaaaaact
    20641 ggaaggaact gctcgatcat ccttgagaac tgctcttctt acttctggtc aagattctcg
    20701 tggttggtgg ataaggatgg ttgataagtt cattggatta gctagtcgaa aacaaccagg
    20761 tgtaattaat gtagaagttt taacaagggg atggagtgat ttattcagtg ataatcataa
    20821 gttcataaat agtaattgag atgtatggtt ggaatacgtt atacctgaac ttgaactacc
    20881 aagatttggt ttagaatttg agaatcgatg tttataaagg gagggcaaaa atgcgttttt
    20941 ttacaacttt tactatcatt atgattgcag tactttttat ttttctagat atagcaaagc
    21001 gaaatacagc atttttatta tatcgggttt tacttcgagc aggtctcata acatttatat
    21061 cgatcgttgg attttttcta ttcacagtta ttgtatttat ttggagaact ccagcaccac
    21121 cattgccaga gattacatat ggagaatttc cttttagact tgagtatgaa ttgaatgaag
    21181 aattacatgt gatagaagac acgttaatag ttgaatttga tggatttgga atgaacgaag
    21241 gcatagggag atatcgtaga tggacaagtc gtttagccag cggagaagat ttagtgttgc
    21301 tgttagaagt tagtgataat aagcaaatat tttattttcc aggacctgct aattattaca
    21361 tgggagatag gttgaatggt tataatcata cttttcctag tgcttctttc attgaaaggg
    21421 aaagaggcat tactagaagg gatattttac atgacaaaga attattagaa caatttggtc
    21481 cgcttgatca aaatacaata aatgaagaag aattgttaaa tcaatataat ataaggctag
    21541 tgaattggga aatatctgaa ccgattgtta acaattttgg tgattagaaa acaattacga
    21601 taaatagaaa caagccaaat catcagcaag taaaagctga tgatttggcc tttcttatac
    21661 gagtagtagg aattcatagc gaatattatt acgatcagta gatattttga tattaaagga
    21721 gtattacgaa ctagtatgtg agaatgtaga ggtgatttgc gaataatgag aaatttatca
    21781 aatagaaaca aaatattaat cattattgtt gttatagctg tgtttcatct gggaacgaat
    21841 gcggttttaa gtagaataat attgggtcct aagccaccaa gacctgcgat tacccgaggg
    21901 gagtttgatt ttagattaga atatgaagta gatggggaaa ggattgtgat agaagataca
    21961 attgttgcat tatttgatgg attttctgca gatgcaggta gcatggcatg gtatcgcacg
    22021 tggcgtttgc atttggcgag tgacaagagg gcacaacaat tattgttaca tgagttagat
    22081 gatggtcgaa aaatatatta tcattttgga ggagctggat tttatatggg gaagttagta
    22141 gcagtgtaac tgtaacaagg aaagatttgc caggtcttgg cattcgaaac tccatatttt
    22201 atatcgagcg tgggagagat acatctattg ggaacaggaa agggttaact cttgaagagt
    22261 tatataatga ctttggaatc cgtcttgtta gttgggaaca caatccacca atagaaaatc
    22321 gctttgaata atcgaaatct attaatccat ttcatcagcc agtgaatgtt gtaatggaac
    22381 aatttggttt agtgttcaaa accattgaaa tactaggctg tgatcagcaa aaatgtatct
    22441 gaataaaaaa gagagcaact attaaatgag aggagttagg agttgttagc tttatacgaa
    22501 tcaaagtggc aaatacagat gcgtatgcat ttgtgtttgc catcttgcgt ttggtagacg
    22561 agataggtat aagctaaaga aacaacttgt ttaaaataaa cgacctagct caacaaacga
    22621 ccatctaatc aactaacagc atacataaaa gcaactattt ttgaagtaga attgaaatcg
    22681 ggataactta aaaagtgatt gggatactag tgtgaatcac cgtacataaa tattactcgt
    22741 taagggaatt tgagaaattt tgtatgaaaa tttaagaatt agttagtatg cgcaagtcaa
    22801 aaatatgaat aaattatttg gtggagataa tttgctcagc ttgtcataag aaatgagata
    22861 atctggcaga tgcaagcttt tggacaccat ttattcggaa taaggaggct gggacagaag
    22921 cgtttaactc caagaaataa gaag
//
LOCUS       DAZKLD010000032        22818 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.032, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000032 DAZKLD010000000
VERSION     DAZKLD010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22818)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22818)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22818
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.032"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            153..269
                     /locus_tag="ACG8KR_001021"
     CDS             153..269
                     /locus_tag="ACG8KR_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HFQ0200410.1"
                     /translation="MREKKQTSTFAKVTKFVIWTMLILTIGSVVLTAVMSVM"
     gene            complement(324..2006)
                     /gene="recN"
                     /locus_tag="ACG8KR_001022"
     CDS             complement(324..2006)
                     /gene="recN"
                     /locus_tag="ACG8KR_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HFQ0200411.1"
                     /translation="MLQEISITNFAIIPELRLSFHEGMTALTGETGAGKSIIIDALGL
                     LAGGRGSSDYIRQGAEKCVLEGLFELPKQEGFSELMVELGIETDEDNLIVRRDMSLTG
                     KNVCRVNGHIITLANLRRIGSYLVDIQGQNEHQELLQPESHLALLDRFGDVAFQQKKK
                     SYQQEYISYRELEKRVRKVQQNEKSYVQRIDMLHFQQEEIAAADLQVGEEEKLKEERE
                     KLSNYQKIADGLAAGYGALTNNEQNSVDGVGLAVSELQGIAHLDVEYEAIYENIQSAY
                     YLLQDAIGDMSRQIDLLELDESRLEEVTQRLELIRQLKRKYGESIESILAYYDEITEE
                     LASSDFSEGQLDKMKEELEQKELLLQQQAADLHEARKKIAKELEKSILHELKSLYMEN
                     TEFEVRFLKEENRQLNHDGFDQIEFYITTNPGEPLKPLVKVASGGELSRMLLALKTIF
                     SSEQGVTSIIFDEVDTGVSGRVAQAIADKISQISKYSQVLCITHLPQVAAVADYQYYI
                     VKAVIDGRTQTSVSELKTKEREEEIARMLAGSEITKLTLEHAKELLKLAKRT"
     gene            complement(2024..2473)
                     /locus_tag="ACG8KR_001023"
     CDS             complement(2024..2473)
                     /locus_tag="ACG8KR_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HFQ0200412.1"
                     /translation="MRKKDRHRLITRLLSEQDIRKQEEFVEILKSKGISVTQATISRD
                     IKELKLIKVPASDGGYRYSIPAETNEDVNTKLEKLLKDAFVAVDQMEKFVILKTLPGN
                     ASAAANLIDKRYKKELFSIINDDDNVLMITRTEDQAVLLKKDFLHYL"
     gene            complement(2580..3398)
                     /locus_tag="ACG8KR_001024"
     CDS             complement(2580..3398)
                     /locus_tag="ACG8KR_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HFQ0200413.1"
                     /translation="MEKERVDVLAVKQGLFETREQAKRSVMAGLIYNEKNERFDKPGE
                     KIPVSSELRVKGKKLPYVSRGGLKLEKALKAFDLSVEGKTLLDIGASTGGFTDAALQN
                     GAKMSYALDVGYNQLAWKIRQDPRVVVMERVNFRYAKPEDFTLGIPEVAVIDVSFISL
                     KLMLPPLHAILKEEGEVIALIKPQFEAGREAVGKNGIVRDPQTHQKVLEDILSFAAQG
                     GYDVLELSYSPITGGEGNIEFLAHLRKVPESGTINSAINMAEVVSNAHEQFDHK"
     gene            complement(3407..4297)
                     /locus_tag="ACG8KR_001025"
     CDS             complement(3407..4297)
                     /locus_tag="ACG8KR_001025"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HFQ0200414.1"
                     /translation="MRLTDFSAIHLPAVEKEILSFLDEHTTDRGLYDAMSYSVKAGGK
                     RIRPLLLLTAVASFDEPIDVPVYQVAAALEMVHTYSLIHDDLPAMDNDDLRRGKPTNH
                     KVFGEALAILAGDGLLTGAFQLISMAHLGNSPKLLLLQQLAVCAGSQGMVAGQAADIE
                     GESKKLSLEELAFIHERKTGHLIRYALLAGGILAKQPEEILLLLQRLAEHLGLAFQIR
                     DDLLDVIGTTKTLGKTAGKDERMEKNTYPRLLGLEKTREALEIELLSANKIIDKLEED
                     VFSFDGGLMRQMIKQFDVEK"
     gene            complement(4299..4523)
                     /locus_tag="ACG8KR_001026"
     CDS             complement(4299..4523)
                     /locus_tag="ACG8KR_001026"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HFQ0200415.1"
                     /translation="MENPTFEESLQELEKIVMQLEQGDVPLESALDSFKRGMELSKHC
                     QDTLAKAEETLTKMMTESNEEVDFDGNEES"
     gene            complement(4528..5865)
                     /gene="xseA"
                     /locus_tag="ACG8KR_001027"
     CDS             complement(4528..5865)
                     /gene="xseA"
                     /locus_tag="ACG8KR_001027"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HFQ0200416.1"
                     /translation="MAQEYLTVTTLTKYLKRKFDADPYLERVYLTGEISNFRLRANAH
                     QYFSLKDDHAKISAIMFKGAFQKLRFQPKEGMKVLVVGRISLYEAGGSYQIYVEHMEP
                     DGIGALYQELEERKEKLMKEGLFQGPKKMLPRFPKRIAVLTSPSGAVIRDIITTVKRR
                     YPIVQLVLFPTVVQGNQAADNVVRNIQRVEEMGNFDTMIIGRGGGSIEDLWPFNEERV
                     ARAIYQANTPVISSVGHETDTTIADLVADVRAATPTAAAELAVPVLSEELMRIQERQA
                     RLQQAFVRQIQRKQERFERARRSYVFRQPERLYEGQTIKLDQLKQRLYQATQQIYHAK
                     EKQTIALSHQLEQVAPLYRVKAARQETAYLEKRLEEKIQQYMKQQQQRFAQAVQSLDL
                     LSPLKIMGRGYSYTTLNDQVVKSVTEIHQGDLLTIHYQDGIVKGQVQEIDKGE"
     gene            complement(5866..6720)
                     /locus_tag="ACG8KR_001028"
     CDS             complement(5866..6720)
                     /locus_tag="ACG8KR_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HFQ0200417.1"
                     /translation="MAELINGKELAEKMQAEIAEKIQKLKDNGIHPGLVVLLVGENPA
                     SQIYVRNKERVAKEIGIHSLVERYPETISEDELLAEIEKYNQDPSFHGILVQLPLPKH
                     IDEEKILLAIRPEKDVDGFHPMNLGRLLAGKPDKIPCTPYGIMKMFEAYSIDPAGKRA
                     VVIGRSNIVGKPMAQLLLMADATVTVAHSKTENLAELAQEADILVVAIGRGHFVTKEF
                     VKPGAVVIDVGMNRDANGKLIGDVKFDEVEPIAGWITPVPKGVGPMTITMLMYQTVKS
                     AEAFGAGE"
     gene            complement(6817..7266)
                     /gene="nusB"
                     /locus_tag="ACG8KR_001029"
     CDS             complement(6817..7266)
                     /gene="nusB"
                     /locus_tag="ACG8KR_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HFQ0200418.1"
                     /translation="MNKELSRHEIREMALQALFPLDFNADLTKEDAIFNAIELDHRDM
                     INEDESEFVPVYLDTLVGGVCAKKDELDKVIEKHLKNNWHINRISKMDLVILRIAIFE
                     MTYVTDVPAAVALNEAIELAKTFSDDRSRKFVNGVLSNVLKELEAGA"
     gene            complement(7259..7684)
                     /locus_tag="ACG8KR_001030"
     CDS             complement(7259..7684)
                     /locus_tag="ACG8KR_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFQ0200419.1"
                     /translation="MADEKNLILNANQDLGEIVIAPEVIEVIIGIAASKVEGVYGMRG
                     TFASNVTELLGRAAHGKGVYLANEEDGLKVDLYCYLEYGVSVPKVAMEMQERVSQQVL
                     FMTDIDLSEVNIHVVAVVPEKLEQPTIEELFEDEEEENE"
     gene            complement(7930..8994)
                     /locus_tag="ACG8KR_001031"
     CDS             complement(7930..8994)
                     /locus_tag="ACG8KR_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HFQ0200420.1"
                     /translation="MMLRVEKLRKKMQEENLDSFLVTSPYNLRYLTNFTGTTGLAVIT
                     LEKAFFITDFRYTEQAAAQAQGFEIIKNVGPIFDEVANLVRKEELHSLAFEETTVSFL
                     EYSVLEEIIDAELVPVSQMIEELREVKDEEEIAIIEKACHIADMAYDHILKMIQPGMT
                     EIEVANQLDFYMRSLGASGVSFDTIVASGLRSAMPHGVASKKIIEQGDLITLDFGCYY
                     EGYVSDMTRTFAIGDPGEKLKEIYQIVLDAQLAVIDAAKPGMTGVQLDAVARDYISKH
                     GYGEAFGHSTGHGIGLEIHEGPNVSSRAEKQFVPGNVITDEPGIYLPGIGGVRIEDDL
                     LITEDGNRVLTRSPKELIIL"
     gene            complement(9166..9411)
                     /locus_tag="ACG8KR_001032"
     CDS             complement(9166..9411)
                     /locus_tag="ACG8KR_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFQ0200421.1"
                     /translation="MSWLWTLIVGAVIGAIAGAITSKGNSMGCVTNIIAGLVGSSIGQ
                     AIFGEWGPKAAGMALIPSILGAVILVAVVSFFFGKRS"
     gene            complement(9550..9924)
                     /locus_tag="ACG8KR_001033"
     CDS             complement(9550..9924)
                     /locus_tag="ACG8KR_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HFQ0200422.1"
                     /translation="MVQSINTKVDLVIDATSFTGLTDYGQIMIGDNGFEFYHARDRRK
                     YIQIPWEEVDYVLASVMLKGKWIPRYAIQTKKNGTYTFSSKEPKKVLRAIREYVDEDR
                     MVQSLSFFDVVKRGIRSMFKRK"
     gene            complement(10007..10921)
                     /locus_tag="ACG8KR_001034"
     CDS             complement(10007..10921)
                     /locus_tag="ACG8KR_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFQ0200423.1"
                     /translation="MAEEKIKLSKKDRLAVAWRSTFIQGSWNYERMQNGGWAFSMIPA
                     IKKLYKTKEERSAALKRHLEFFNTHPYIASPILGVTLALEEERANGAPVDDVAIQGVK
                     VGMMGPLAGVGDPVFWFTVRPMLGALGASLAMGGNILGPIIFFLGWNIIRWAFMWYTQ
                     EFGYKAGSKITDDLSGGLLQDVTKGASILGMFVLAALVERWVSIKFLPVVSTVKLDKG
                     AYIEWDKLPSGGEGIKSAFEQVNNGLALSPEKVTTLQDNLDQLIPGLAALGLTLLCMW
                     LLKKKVSPIVIILGLFVVGVVGHVIGLL"
     gene            complement(10942..11745)
                     /locus_tag="ACG8KR_001035"
     CDS             complement(10942..11745)
                     /locus_tag="ACG8KR_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HFQ0200424.1"
                     /translation="MSIISIILVVLVAFLAGMEGILDEFQFHQPLVACTLIGLVTGNL
                     EAGIVLGGTLQMIALGWANIGAAVAPDAALASVASAIILVLGGQGVKGVPSAIAIAVP
                     LAVAGLFLTMIVRTIAVPIVHLMDAAAEEGNIRKVEMWHIIAVCLQGVRIAVPAAALL
                     FIPAQTVQSFLESMPAWLTDGMAIGGGMVVAVGYALVINMMATKEVWPFFVIGFVVAA
                     ISQLTLIALGALGVALALIYLNLSKMGGSSNNGGGSNSGDPLGDILNDY"
     gene            complement(11778..12767)
                     /locus_tag="ACG8KR_001036"
     CDS             complement(11778..12767)
                     /locus_tag="ACG8KR_001036"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose/fructose/sorbose PTS transporter subunit
                     IIB"
                     /protein_id="HFQ0200425.1"
                     /translation="MVGIILASHGQFAEGILQSGSMIFGEQENVKAVILKPSEGPDDL
                     RAKLEEAVASFDNQDEVLFLVDLWGGTPFNQSNTLFEEHKDKWAIVSGLNLPMLIEAY
                     ASRFSMESAHEIAAHIIETAKDGVKVKPEELEPAEAPKAAVEDAQPKGALPEGTVVGD
                     GKIKYVLARVDSRLLHGQVATAWTKAVQPNRIIVVSDAVSKDDLRKRLIEQAAPPGVK
                     ANVIPISKMIEVAKDPRFGNTKALLLFENPEDVLTAVEGGVDIKELNVGSMAHSVGKV
                     VVSKVLSMGQEDVEAFEKLEQKGVKFDVRKVPNDSRDNMDDILKKAKAELAKA"
     gene            complement(12870..13361)
                     /locus_tag="ACG8KR_001037"
     CDS             complement(12870..13361)
                     /locus_tag="ACG8KR_001037"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose/fructose/sorbose PTS transporter subunit
                     IIB"
                     /protein_id="HFQ0200426.1"
                     /translation="MDIRLARIDDRLIHGQVATVWSKQTGVERIIVISDVVAQDKLRK
                     FLLKEAAPPGITANVITVQKMIQIYHSGLLSRAKVMLLFTNPQDVEAVVRCGVQLKSL
                     NIGGMSFTEGKSMITNFVSVNQQDIDSFRYLNSQEVELEIRKVPADRKILLMELLAKI
                     KKV"
     gene            complement(13552..16359)
                     /locus_tag="ACG8KR_001038"
     CDS             complement(13552..16359)
                     /locus_tag="ACG8KR_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFQ0200427.1"
                     /translation="MYVKEKTAHLTKAEYDQGVTTQEIAEVLGIQRTNSSKDLNQLVR
                     EGKLIKTDGRPVRYIYQTLVTHVKPVTKHVVSYKETPVSEEKNTLQIDTKDIFAKIIG
                     ANGSMKNSVEQAKAAILYPPRGLNCLITGPTGSGKTYFAHAMYHFAKAHHVIDEETEL
                     IVFNCADYANNPELLMSHLFGYAKGAFTGADEEKMGIIDQADGGMLFLDEIHRLPPEG
                     QEMIFYFMDHGTYSRLGETTKSHEANVRIVGATTEDPGSSLLETFVRRIPINIKLPAF
                     EKRPANEKVDLVKIMIAHEANRTQRKISLTEDVAKALIGSVTYGNIGQLKSNIQLVCA
                     RGFLNHMQSPEISITIDDLTEGIRSGLIQLASNRSAMAELSKILEPKITVAPNDTIMK
                     IQSDSYELPYNLYDIIGDKAALLKSDGLDQEAINHFISTDINIHLKSFYKDHGFSFNA
                     DSKLAEFVDPKIIEVTNQIYVMVKRSLPYEFQQNFIYAMSLHISSFLKKINLGEERHT
                     NDNIREMAFDFPDEYAVAKEVRSYIETYFQVEIPESEDYYLTVLLVSLRANQASGRIG
                     VVVAAHGNSTASSMVQVVQQLLDAENVRAVDMPLDMDPKTALDRIERNVQEVDEGSGV
                     ILLVDMGSLASFNSQIQRDTGIPVRTVDMVTTSLVLETVRKVSVLGTDLDMLHESLKN
                     FRGYAEISSVESVQEAKPIHKKKAVLAICASGEGTAQRIKELIERAVNKRQETELEVL
                     ALSIVEVKEELPVIQKEYQIIATTGITDLNIQAPFIPLERFIDQNIEVILDQLLMESE
                     LEETEFISLDEESAKNTCVEFISDNFIFINGSKLIDPMWQFSTQISQTTGIGDEEYGF
                     KINLVMHTAGMIERIIRNEPLTVEENELTNTTNDPLYSQLAASVVLLEDQIKVKVPIE
                     EMYYLLRLVHNQLDKKEYTVP"
     gene            complement(16793..17086)
                     /gene="rpmA"
                     /locus_tag="ACG8KR_001039"
     CDS             complement(16793..17086)
                     /gene="rpmA"
                     /locus_tag="ACG8KR_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HFQ0200428.1"
                     /translation="MLLSMNLQLFAHKKGGGSTSNGRDSESKRLGAKRADGQTVTGGS
                     ILYRQRGTKIYPGVNVGIGGDDTLFAKVDGVVRFERKGRNKKQVSVYPVAQEA"
     gene            complement(17099..17443)
                     /locus_tag="ACG8KR_001040"
     CDS             complement(17099..17443)
                     /locus_tag="ACG8KR_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HFQ0200429.1"
                     /translation="MIKGTFKRNDAGQIVSFTLTGHADAGPYGSDIVCAGVSALAIST
                     VNGIASLAGFEPIVEMNEEEGGYLYTEVTSGMTQEQNNIAEILLENLLLGLQSIEAEN
                     SEYIQIKTINDK"
     gene            complement(17470..17778)
                     /gene="rplU"
                     /locus_tag="ACG8KR_001041"
     CDS             complement(17470..17778)
                     /gene="rplU"
                     /locus_tag="ACG8KR_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HFQ0200430.1"
                     /translation="MYAIIKTGGKQVKVEVGQAIYVEKLDVEAGEKVVFDEVILVGGE
                     STKVGAPTVAGATVEGTVEKHGKQKKVVTFKYKPKKHSHRKQGHRQPYTKVVIDAINA
                     "
     misc_feature    complement(17798..17874)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            18060..18701
                     /locus_tag="ACG8KR_001042"
     CDS             18060..18701
                     /locus_tag="ACG8KR_001042"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HFQ0200431.1"
                     /translation="MEYPKELIQEASLRMNGRPVEGFIAGQGPLHPKLMLVGEAPGKT
                     EIDTHVPFSGQAGKELMQALSSTGLTREEVYITSAVRSRPYRVTHRINKRTQQSETVY
                     PNRTPTRSEVFAHAPILDYELMKVQPKLIATLGNIGLQRLLGKEYTVTKNHGQLYTGP
                     VLQLTEQKDAYEWSEKNYRILPLFHPAAIFYNRSLVPVIHQDWQVLASLLQQY"
     gene            complement(18763..20394)
                     /locus_tag="ACG8KR_001043"
     CDS             complement(18763..20394)
                     /locus_tag="ACG8KR_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015103 - inorganic anion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HFQ0200432.1"
                     /translation="MFKNYVSSLKKEFSGYDLSRFQKDLLAGITVAAVALPLALAFGV
                     SSGADAAAGLITAIIAGIVIGGLSGGFYQISGPTGAMAAILMSIAATKGMQGVLLATF
                     LAGLFLLAAGLFRLGTLTSFIPAPVITGFTSGIAIIIALGQIDNLFGVHSEGTNVIEK
                     LSSYQTIGFPINTASLIMGGLVILGMFIYPKKWAKRMPSSLLAIILATAVMLLFHLPV
                     ATVGKIPQTLISSNRLNMGDFSFSALQQVAVPAISIALLGMIESLLCGASAGRMANKP
                     LDSNQELMAQGIGNLLLPFFGGIPATAAIARTSVAIKSGAQTRLAGMIHAVVLLLSML
                     VFAPVMSQIPMPALAGVLIVTAWRMNEWETIKELFTNKYWSAVLLFILTMICTVIFDL
                     SIAIVIGVIGGCVFFVVKSAAITISLETIDWQRMGLPETEKLNNWTVVYISGPLFFMS
                     SEKLKNKLEELNHHDGVIFSMRGVPSIDLTARAVFDEFQEKAEQRGQTIIYTSLQPEV
                     EKQLSHLWEHTFTHQHQTVAHALTALHAKYTAISH"
     gene            complement(20517..20876)
                     /locus_tag="ACG8KR_001044"
     CDS             complement(20517..20876)
                     /locus_tag="ACG8KR_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HFQ0200433.1"
                     /translation="MTILSRILVTLVAIEFFYIMYIETVRTDSDTTSRVFKMSKEELS
                     RKSVQTLFKNQGVYNGLLGVGLLYGAYLSSASKEITSMLLISIFFVALYGSLTSDRWI
                     LLKQGGLALLALLSIFL"
     gene            complement(21035..21108)
                     /locus_tag="ACG8KR_001045"
     tRNA            complement(21035..21108)
                     /locus_tag="ACG8KR_001045"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(21072..21074),aa:Arg,seq:tct)
     gene            complement(21417..22100)
                     /locus_tag="ACG8KR_001046"
     CDS             complement(21417..22100)
                     /locus_tag="ACG8KR_001046"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HFQ0200434.1"
                     /translation="MRNEMMHRPVVKEELLDFMRTQQKKLPGELGKLEEEAHVAEVPV
                     IPHETVVFLKFLLGQLQPERILEIGAAIGFSSSVMATTIGENAHVTTIDRFDVMIEKA
                     KKNYERLGLTDKVTLLEGQAADILPELSGPYDFIFMDSAKSKYIEFLPECLRLLRKGG
                     VLMVDDIFQGGTILLPDEEIPRGKRAIHRKLNEFLRVVMDHPDLTSTILPLGDGVILM
                     TKESETIEL"
     gene            complement(22228..22746)
                     /locus_tag="ACG8KR_001047"
     CDS             complement(22228..22746)
                     /locus_tag="ACG8KR_001047"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFQ0200435.1"
                     /translation="MPDYKKITFISSITLIIFLILILLVVLVPRIFGLTPYIIEDKEM
                     SPYYAQGNLIYVRPKNTNEILVGDIITYYENSGKKISTRRVIAVENNHEDFYTKADTK
                     TYIEPGVVKKRNIIGSPIFQIPYIGLVLSKTAFAWIQGLFFTIAFCLTGITAWNTFVE
                     LKKKRRRTQKFL"
BASE COUNT         6563 a         5033 c         3792 g         7430 t
ORIGIN      
        1 cctttatatg gctagttcta ttaacattat ttatttgtat cgtaactacc tattatttaa
       61 aaaaagggaa gtttttataa aaaagccctc ttgtggaaat ggaaaaaaga ttgtacactt
      121 gaatcaacta attaatgacg aggttgaaaa caatgcgtga aaaaaaacaa acaagtactt
      181 ttgctaaagt aacaaaattt gtcatttgga caatgctgat tttaacgatt ggatctgttg
      241 tattgactgc tgtaatgagt gtcatgtaaa aataaaatat agctatcagc tgctgaaaag
      301 gacctgaacc actgcgaggt ggctcaggtc ctttttgcga gtttcaatag ttcttttgca
      361 tgttcgagtg tgagttttgt aatctcactt ccagccagca tacgtgcaat ttcttcttct
      421 ctttcttttg ttttcaactc tgaaacagaa gtttgcgtac gtccatcaat cactgctttc
      481 acaatataat attgatagtc ggcgactgca gcaacttgag gcagatgagt gatgcacaaa
      541 acttgagagt atttcgatat ttgagaaatt ttatctgcga tggcctgtgc aactcttccg
      601 ctcacacctg tatccacttc atcaaaaata atacttgtca caccttgctc agaagaaaaa
      661 atagttttca aagcaagcaa catccgagac agttcgccac cagaagcgac tttcaccaaa
      721 ggcttcagtg gctctcctgg attggtcgta atgtaaaatt cgatttggtc aaatccatca
      781 tgattcaatt gtctattttc ttcttttaag aaacgtacct caaactcagt gttttccata
      841 tacagacttt tcaattcatg cagaatggat ttttccaatt cttttgcgat ttttttacgc
      901 gcttcatgaa gatctgcggc ttgttgctga agtagaagct ctttttgctc tagttcctct
      961 ttcattttgt ccaattgacc ttctgaaaaa tcagaagaag ctagctcttc ggtgatttcg
     1021 tcatagtaag ccaatattga ctcaatggat tcgccgtatt tacgtttcaa ctgtcggatt
     1081 aattctaaac gttgcgtaac ttcttctaaa cgactctcat ctaactccaa taaatcaatc
     1141 tgacgactca tatcgccgat tgcatcttgc agcaaatagt aagcactttg gatattttca
     1201 taaattgctt catactccac atctagatgg gcgatccctt gcagttctga aacggccaag
     1261 ccaacaccat ctacactgtt ttgttcatta ttcgtcagtg ctccgtaacc tgcagctaat
     1321 ccatcagcaa ttttttgata attgctaagt ttttctcttt cttcttttaa tttctcttct
     1381 tctccgactt gcaaatcagc agcagctatt tcttcttgct ggaaatggag catatctatt
     1441 ctctgaacat aggatttttc attttgctgc acttttcgta cccttttttc cagctcacga
     1501 taagagatat attcttgctg ataactcttt ttcttctgtt ggaaagctac atctccaaaa
     1561 cgatccaata aagcaagatg tgattcaggc tggagcagtt cctgatgttc gttttgtcct
     1621 tggatatcta ctaaataact gccgattctt ctcagattag ctaatgtgat aatatgacca
     1681 ttcactcgac aaacattttt tccagttaag gacatatccc gtcgaacaat cagattgtcc
     1741 tcatctgttt cgatccctaa ttcaaccatc agttcagaga atccttcttg ttttggtagt
     1801 tcaaacaacc cttcaagcac acatttttct gctccttgac gtatataatc gcttgaacct
     1861 cttcctcctg ctaacagacc taaagcatcg ataataatgg atttacccgc accagtctct
     1921 ccggtcagcg cggtcattcc ttcgtgaaaa gaaagccgca attccggaat aatcgcaaag
     1981 ttagtaatac tgatttcttg tagcatcaca ttcgctcctc aacttaaagg taatgcagga
     2041 agtctttctt caataaaact gcctggtctt cagttcgtgt aatcatcaaa acattatcgt
     2101 catcattgat aatcgaaaat aattctttct tgtaacgttt atcaatcaaa tttgctgctg
     2161 ccgaagcatt tcccggcaat gttttcaaaa taacaaattt ttccatttgg tcaactgcca
     2221 cgaaagcgtc ttttaatagt ttttccaatt tagtatttac atcttcgttt gtttcagctg
     2281 gtatgctata acggtagccg ccatctgaag ccggtacctt gatcagtttc agttccttta
     2341 tatctctgga aatcgtagct tgagtaacag atattccttt gcttttcagg atttcaacaa
     2401 attcttcctg ctttcggata tcttgctcac tcaacaatcg agtaatcaaa cgatgccgat
     2461 ctttttttct cattacgtct tcgctcctta gcatattctt cttaatcaat gatagcttat
     2521 ttccttggta taataaagaa gaatccgcat acttctcact tgattttcac agttacaatt
     2581 tatttatggt caaactgttc gtgtgcattt gaaaccactt ctgccatatt aatagctgaa
     2641 ttgattgttc cgctttcggg tactttcctc aagtgagcca agaactcgat atttccttct
     2701 ccccccgtga tcggagaata acttaattcc agcacatcat atccaccttg tgccgcaaat
     2761 gacaaaatgt cttctaaaac tttttgatgg gtctgagggt cacgaacaat tccgtttttc
     2821 cctacagctt ctctgcctgc ttcgaattgg ggtttgatta aagcaatcac ttctccctct
     2881 tcttttaaaa tagcatgtaa tggaggcagc atcagtttta gtgaaataaa agaaacatcg
     2941 ataacagcaa cttctggaat tcctaaggta aagtcttcgg gttttgcata acgaaaattc
     3001 actctttcca ttacgacgac tctaggatct tgtcttattt tccaagctag ctgattgtag
     3061 ccaacgtcta aagcataact catttttgcg ccattttgca aagctgcatc agtaaatccg
     3121 ccagtggatg caccaatatc taacaatgtt tttccttcaa cagatagatc aaatgctttg
     3181 agtgcttttt ctaattttaa tccccctcga gaaacataag gaagtttttt acctttgact
     3241 cttaattcac tactcactgg aattttttct cccggtttat caaaccgctc gtttttttcg
     3301 ttgtaaatca aaccagccat cactgaacgt ttggcttgtt ctcttgtttc aaataatcct
     3361 tgtttgaccg ctaatacatc tacgcgttct ttttccattc tttccctcat ttctccacat
     3421 caaattgctt gatcatctgg cgcatcagcc cgccatcaaa agaaaaaacg tcctcttcta
     3481 atttgtctat gatcttgttg gcggataaca gctcaatctc caacgcctca cgtgtctttt
     3541 ctaggcctaa taaacgcggg taagtatttt tttccatacg ttcatcttta ccagctgttt
     3601 tccctaatgt tttcgttgta ccaatcacat cgagtagatc atctcttatc tgaaaagcta
     3661 aacctaaatg ttcggctaat cgttgaagta gcaataagat ttcttcaggt tgcttcgcta
     3721 atatgccacc agccagcaag gcataacgaa tcaaatgccc tgtctttctt tcatgaataa
     3781 atgctaattc ttctaaagaa agctttttcg attcgccctc gatatctgca gcttgcccag
     3841 caaccatacc ttgtgatccc gcacaaacag ctaattgctg aagcagcaga agcttaggag
     3901 aattacctaa gtgtgccata ctgattagct ggaatgctcc tgtaagcaaa ccatctccgg
     3961 ccaatatagc taatgcttcg ccgaatactt tatgattggt aggttttcct ctacgaagat
     4021 catcattgtc catcgcaggc aaatcatcgt ggatcaagga atacgtatgc accatttcta
     4081 aagcagctgc tacttgatag acgggaacat caataggctc atcaaaggaa gcaacggccg
     4141 ttaaaagtaa taagggacgt attcttttcc cgcctgcttt cactgaataa ctcatcgcat
     4201 catagagtcc tctatccgtt gtatgctcat ctaaaaaaga caagatttct ttttctacag
     4261 caggtagatg aatagctgaa aagtcagtta atctcatctc agctttcctc gtttccgtca
     4321 aaatcaactt cttcatttga ttctgtcatc attttcgtca aggtctcttc tgctttagct
     4381 aatgtatctt ggcaatgttt gcttaattcc attcctcgtt taaacgaatc tagagctgat
     4441 tctaatggaa cgtctccttg ttctaactgc atgacaattt tttctaattc ttgcagtgac
     4501 tcttcaaaag ttggattttc catattttta ttctcctttg tctatctctt gtacttgtcc
     4561 tttaacgatt ccatcttgat aatggatcgt cagcaaatcc ccttgatgaa tctcggtgac
     4621 actttttact acttgatcat ttaacgtcgt atagctgtag cctcttccca tgattttcaa
     4681 tggactcaac aaatcaagag attgaacagc ttgagcaaaa cgctgctgct gttgtttcat
     4741 atattgttgg attttctctt ctaagcgttt ttctagataa gcagtttctt ggcgagctgc
     4801 tttaactcga taaagcggag ccacttgttc caattggtgg cttaatgcga tcgtttgttt
     4861 ttctttcgca tgataaattt gctgtgtagc ttgatataat cgctgtttca actggtctaa
     4921 tttgatggtc tgaccttcat acaatctttc aggctgccga aatacatagg aacgtcgagc
     4981 acgctcgaac cgttcttgtt tgcgctggat ctggcgtaca aacgcctgct gcaatctagc
     5041 ttgccgttct tgaatgcgca tcaactcttc tgacaaaaca ggaacagcta attctgcagc
     5101 tgctgttgga gtggcagccc gaacatctgc tactaaatca gcgatcgtgg tatctgtttc
     5161 atgtcctaca gatgaaatca ccggcgtatt agcttgataa atcgcccgag caacccgctc
     5221 ttcattaaac ggccataaat cctcaatcga accaccccca cgaccgatga tcattgtatc
     5281 gaaattgccc atctcttcta ctctctggat attcctgaca acattgtccg ctgcttggtt
     5341 tccttgtaca actgttggga acaaaaccaa ttggacaatt ggatatcttc ttttcaccgt
     5401 cgtgatgata tcgcggataa ccgctccgct tggactggta aggaccgcga ttcgctttgg
     5461 gaaacgtggc agcatttttt taggcccttg aaaaaggcct tccttcatca gcttttcttt
     5521 tcgttcttcc aattcttgat ataaagcacc tattccatca ggctccatgt gctctacata
     5581 gatttgataa gaacctcccg cttcatataa tgaaatacgc ccaacgacaa ggactttcat
     5641 tccttctttt ggctggaatc gtaatttttg gaatgctcct ttgaacataa tagcagatat
     5701 ttttgcatgg tcatctttta agctgaaata ttgatgggca ttggcacgta aacggaaatt
     5761 cgatatttcg cctgttaaat atacgcgttc taaataagga tcggcatcaa attttctttt
     5821 caaatacttc gttaatgtag tgacagttaa atattcttgt gccatctatt cacctgctcc
     5881 aaaagcttcg gcagatttta cggtttgata catcagcatc gtgatagtca ttggccctac
     5941 accctttggc acaggagtga tccagcctgc aataggctcc acttcatcaa atttcacatc
     6001 accgatcagc ttaccgttag catcacgatt catccctaca tctatcacta cagcacctgg
     6061 ttttacgaat tcttttgtga caaaatgtcc acgtcctatt gctacgacta aaatatctgc
     6121 ttcttgtgcc agttcagcca gattttcggt tttagaatga gcaacagtaa cagttgcatc
     6181 tgccatcaag agtagctgag ccatcggttt accgacgata ttgcttcgtc cgataactac
     6241 tgctcgttta cccgccggat cgatagaata agcttcaaac attttcatga tgccgtatgg
     6301 agtacaagga atcttgtccg gtttacccgc aaggagtctg cctaaattca tcggatgaaa
     6361 accgtctacg tctttttcag ggcggatagc taacaggatc ttttcttcat ctatatgctt
     6421 tggcaatggt aattggacca atataccatg aaaactagga tcttgattgt atttttcaat
     6481 ttctgcaagt agttcgtctt cagaaatcgt ctcaggataa cgctctacca atgaatggat
     6541 tccgatttct tttgctacac gttctttatt tcttacatag atttggctgg ctggattttc
     6601 ccctaccaat aaaacgacta atccgggatg gataccatta tctttcaatt tttggatttt
     6661 ttcagcaatc tctgcctgca ttttttcagc taattctttt ccgttaatca attctgccat
     6721 ctaaccactc ccagatattt tttgtgtttt cattctacca tagaattttc ccgatttata
     6781 acaggtttat gatgattcat agaaaaaagc taggacttac gccccagctt ctaattcttt
     6841 caatacattg gataaaacac cgttgacgaa tttacgtgaa cggtcatcac taaatgtttt
     6901 tgccaactcg attgcttcat ttaaagcaac agccgctggt acatctgtca cataagtcat
     6961 ttcaaaaatc gcgatacgca agatcaccaa atccattttt gagatccgat tgatgtgcca
     7021 attgtttttt aaatgttttt caatcacttt atccaattca tctttttttg cacagacacc
     7081 gccaactagc gtatcaagat aaacaggaac aaactctgat tcatcttcgt tgatcatgtc
     7141 gcggtggtct aattcgattg cgttgaaaat cgcatcttct tttgttaggt ctgcattgaa
     7201 gtctaatgga aataaagcct gcaatgccat ttcccgaatc tcgtgtcttg ataattcttt
     7261 attcattctc ttcctcttcg tcttcaaaaa gttcttcgat tgttggttgt tctaattttt
     7321 caggaacaac agctactaca tggatattga cttcagataa atcgatatcc gtcatgaaaa
     7381 ggacttgctg gctgacccgt tcctgcattt ccattgctac tttaggaact gaaacaccat
     7441 attctagata acagtaaaga tctactttca agccatcttc ctcgtttgct aagtaaacac
     7501 cttttccgtg agctgcacgt cctaataatt cagtgacatt gctggcaaaa gttccgcgca
     7561 ttccgtatac gccttctact ttagaagctg caatcccgat aattacttcg atgacttcag
     7621 gagcgatcac gatctctcct aaatcttggt ttgcgtttaa aatcaaattt ttttcatctg
     7681 ccatttattt attcctccct ttgcctaagc acgagataca tccgaattct ctatgatgcg
     7741 gcatagtgat cacgtggtaa gaaaactgcc gccgatcagc tgcaaactgc tatgaattgg
     7801 agaaaacacc caactttatt atctcttaca gcttaaaagt tcttctatag tgtatcatta
     7861 aatcgagtga ttttctataa caaagtctgt gaacactgat ttttacgctt acttcttcta
     7921 ttgatgaaac tataagatga tcaattcttt tggtgaacga gtcaacacac gatttccatc
     7981 ttctgtgatc aacaagtcat cctcaatacg tactccaccg attcctggta agtagatccc
     8041 tggttcatca gtgatcacat ttcctggtac aaattgcttt tctgcacgag aagaaacgtt
     8101 tggtccttcg tggatctcta aaccgatccc atgtcctgta gaatgtccaa atgcttcccc
     8161 gtaaccatgt tttgaaatat aatctctagc aactgcatct aattggacac cagtcattcc
     8221 tggttttgca gcatcaataa ctgctaattg agcatctaag actatttgat aaatttcttt
     8281 taatttttcc ccaggatcac caatagcgaa tgtacgtgtc atatcggaaa cgtacccttc
     8341 gtaatagcaa ccaaagtcca atgtgatcaa gtcaccttgt tcgatgattt ttttgcttgc
     8401 tacaccatgc ggcatagccg aacgcaatcc gctagcaacg atcgtatcaa aagaaacacc
     8461 tgaagcgcca agagaacgca tataaaagtc tagttgattt gcgacttcaa tctcagtcat
     8521 gcccggttgg atcattttca ggatgtgatc atatgccata tccgcaatat gacatgcttt
     8581 ttcgataata gcgatttctt cttcatcttt cacttctctg agttcttcga tcatctgtga
     8641 aacaggtact aattccgcat cgatgatttc ttctaaaacc gaatattcta aaaatgagac
     8701 tgttgtttcc tcaaaagcta atgagtgcag ttcttctttg cgtactaagt ttgctacctc
     8761 atcaaaaata ggtccgacat ttttaataat ttcaaaccct tgagcctgcg ctgccgcctg
     8821 ctcggtataa cgaaaatcag taataaaaaa tgctttttcc aaagtaataa cagctaatcc
     8881 tgtcgtacct gtaaagttcg ttaagtaacg aagattgtac ggactggtaa ccaaaaatga
     8941 atccaaattt tcttcttgca tttttttgcg caatttttct acacgtagca tcataatgaa
     9001 actccctctt tctttgcacg cttttttcca ttatagcaaa cttttcttcg tacacgacag
     9061 ttaaataaaa atttccggaa aacttctttt tattttaaat aaataaagcc aaaaaggagt
     9121 cacagtctcg ctgcaactcc ttttagttta ctattttgta aaaacttatg atcgttttcc
     9181 aaagaaaaat gagacaactg ctaccaaaat cactgcgcct aaaatagatg gaataagtgc
     9241 catacctgca gcttttggtc cccattcgcc gaaaatcgct tgtccaatac ttgaaccgac
     9301 tagtccagca atgatattcg ttacacagcc catcgaattt cctttactgg tgatcgcacc
     9361 tgcaattgct ccgatcacgg ctccaacaat caatgtccat aaccaactca taatataacc
     9421 tccttctttg ctaatcaatt ttagcatatg ggatttaaag gggacaaata attggttttg
     9481 ctatttcata ttttcttctg caaagtaaaa aaataaagaa aacctcctct tagaagtttt
     9541 ctttattttt tacttgcgtt taaacatact acgaatacca cgtttcacta catcaaagaa
     9601 actcaatgat tgtaccattc tatcttcatc tacatattct ctgatcgcac gaagcacttt
     9661 tttaggttct ttggaagaaa atgtgtatgt accatttttt ttcgtttgaa tggcatatcg
     9721 aggaatccat tttcccttta gcatgactga tgctagtaca taatccactt cttcccatgg
     9781 aatttggata tatttgcgtc tatctcgcgc atggtaaaac tcaaaaccat tatcacctat
     9841 catgatttgc ccgtaatctg tcagacccgt aaacgaagta gcatcaatta ccaagtctac
     9901 ttttgtattg attgattgaa ccattttctc cctccagata tttgtttatg aacagtatac
     9961 tatataaaaa aagctcggga caacgcccga gctatatttt tatggattat aaaagtccga
    10021 tgacgtgtcc aacaacaccg actacgaata aaccaaggat gataacgatt ggagaaacct
    10081 ttttcttaag caaccacata cataacaatg ttaatcctaa tgcagctaaa ccaggaatca
    10141 attggtctaa gttgtcttgt aatgttgtta ccttttcagg agaaagggct aagccattat
    10201 taacttgttc aaatgcactc ttgattcctt caccacctga aggcaattta tcccattcga
    10261 tgtaagcacc tttatctaat tttacagttg atacaactgg taagaatttg attgataccc
    10321 atctttctac aagggcagct aatacgaaca tccctaaaat tgaagcacct tttgttacgt
    10381 cttgtaacaa tccgccagaa aggtcatcag taattttaga accagctttg tagccaaatt
    10441 cttgtgtata ccacatgaat gcccaacgaa taatgttcca acctaagaag aagatgattg
    10501 gtccaaggat atttcctccc atagctaatg aagcacctag tgcacctagc attggacgta
    10561 ctgtaaacca gaacactggg tcaccaacac cagctaaagg acccatcata cctactttaa
    10621 ccccttgaat cgctacatca tcaacaggcg cgccattcgc acgttcttct tccagagcca
    10681 atgtaacccc taaaattgga gaagcaatgt atgggtgtgt attaaagaat tccaagtgac
    10741 gttttaatgc tgcagaacgt tcttcttttg ttttgtataa ttttttgatt gctggaatca
    10801 tagaaaatgc ccagccaccg ttttgcatac gttcgtagtt ccatgaacct tgaatgaagg
    10861 tagagcgcca cgcaacagct aaacgatctt tttttgataa tttgattttt tcttctgcca
    10921 ttttattttc ctccttcaag attaatagtc attcaaaatg tcgcctaaag ggtcaccaga
    10981 gttgcttccg cctccattat ttgatgaacc acccatttta gaaaggttca agtaaataag
    11041 agctaatgca acacctaaag cacctagagc aatcaatgtt aattgagaaa ttgcagctac
    11101 aacgaaacca ataacgaaga atggccatac ttcttttgta gccatcatgt tgattaccaa
    11161 agcgtaacct acagctacga ccattccccc accgatagcc ataccatctg ttaaccatgc
    11221 tggcattgat tcaaggaatg attgcacagt ttgtgctggg ataaatagca acgctgctgc
    11281 tgggaccgcg atacgaaccc cttgtaaaca tacagcaatg atgtgccaca tttcaacctt
    11341 acggatgttt ccttcttcag cagccgcatc catcaaatga acgattggaa cagcgattgt
    11401 acgtacgatc attgttaaga acaaccctgc aacagccaaa ggaacggcaa tcgcgatcgc
    11461 agaaggaaca cctttaacac cttgtccacc taaaactaaa ataattgctg atgctactga
    11521 tgctaatgca gcatctggtg ctacagcggc accgatattt gcccaaccta aagcgatcat
    11581 ctgaagcgta ccgccaagta cgataccagc ttcgaggtta ccggtaacta aaccgatcaa
    11641 tgtacatgcc actagtggtt gatgaaattg aaattcatca agaatacctt ccataccagc
    11701 tagaaaggca actaatacga ctaaaattat tgaaataata gacatgataa gcctcctata
    11761 aattttaata ttatttatta cgcttttgct aattcagctt ttgctttttt caaaatatca
    11821 tccatgttat cgcgtgaatc attaggtact ttacgtacat cgaatttcac acctttttgt
    11881 tcaagcttct caaacgcttc aacgtcttct tgtcccatag acaatacttt acttacaacg
    11941 actttaccga ccgaatgcgc cattgaccca acatttaatt ctttgatgtc tacgccacct
    12001 tcaacagctg tcaatacgtc ttcaggattt tcaaataaaa gcaatgcttt ggtatttcca
    12061 aaacgagggt cttttgctac ttcgatcatt ttgctaatag gaataacatt tgcttttaca
    12121 cctggaggag cagcttgttc gattagtctc ttacgcagat catcctttga tactgcatct
    12181 gaaacaacga taatacggtt tggttgcact gcttttgtcc aagctgtcgc aacttgtcca
    12241 tgtaacagac gtgagtcaac acgtgctaat acatatttga tttttccgtc tccgaccaca
    12301 gttccttctg gaagtgctcc tttaggttgt gcgtcttcaa ctgctgcttt cggtgcttct
    12361 gccggttcta attcttccgg ttttactttt acaccgtctt ttgctgtttc aatgatgtgt
    12421 gctgcaattt catgagcaga ttccattgaa aagcgtgatg cgtaggcttc aatcaacatt
    12481 ggtaaattca aaccgctgac gattgcccat ttatctttgt gctcttcgaa taatgtgttt
    12541 gattgattga acggtgttcc gccccataaa tcaactaaga ataacacttc gtcttgatta
    12601 tcaaaagaag caaccgcttc ttccaatttg gctctcaagt catctggacc ttcacttggt
    12661 ttcaaaatca cggctttgac gttttcttgt tcgccaaaga tcattgaacc ggattgtaag
    12721 attccttcag caaattgtcc atgacttgct aggataatcc ctaccatatt tatacctcct
    12781 atttactttt gttgtaacaa attcaaaaat attaataatt gctaaccatt acaacagaat
    12841 tatttgacct tttccaacaa ccattcttgc tacacctttt tgattttcgc tagtagttcc
    12901 atcaaaagga ttttcctgtc agcaggtact tttcgaatct ctaactccac ttcttgactg
    12961 ttcaagtaac gaaaggaatc aatatcttgt tgatttactg aaacaaaatt ggtaatcatt
    13021 gatttgcctt cagtaaaact cataccgccg atattcaacg acttcaactg tacaccgcag
    13081 cggacaaccg cttcaacatc ttggggattg gtgaataaaa gcatcacttt tgctcgtgat
    13141 aataatccac tatgatagat ttgaatcatt ttctgcacgg ttattacatt tgctgtgatc
    13201 cccggcggtg cagcttcttt tagtagaaac tttctcaatt tatcttgagc gactacatcg
    13261 ctgatcacaa tgatacgttc aacacctgtt tgcttcgacc aaactgttgc gacttgcccg
    13321 tggattaaac gatcatctat acgtgccaat cgaatatcca tctgcaaaac tccttcttcc
    13381 tcactacttc acagaaaccc ctatcatttc tgtgtgaaca accacctcct agcacacact
    13441 actataagca aaaagcgtgc caactttttt acgatacact aattgaattt aaaggattct
    13501 taaccaatac acaaaaacga tacactataa aagtgtatcg tttttgtgta ttcatggcac
    13561 agtgtattct tttttatcta actgattatg gactagacgt agcagataat acatttcttc
    13621 tatcggcact tttactttta tctgatcttc caacagaaca actgaggctg ccaattggct
    13681 ataaagagga tcattcgttg tatttgtcaa ttcgttttct tccacagtca atggttcatt
    13741 ccgaataata cgttcgatca ttcctgctgt gtgcatcact aggttgattt tgaatccata
    13801 ttcttcatcg ccgattcccg tcgtctgaga gatttgtgta gaaaattgcc acattggatc
    13861 gattaactta ctaccattaa taaagatgaa gttatcagag ataaactcaa cacacgtatt
    13921 tttagctgac tcttcatcta gggagatgaa ttcagtttcc tccaattctg attccatcag
    13981 caactgatcc aaaatgactt cgatattttg atcaataaaa cgttctaaag gaataaatgg
    14041 ggcttggata ttcaaatcag taatcccagt agtagctatg atctgatatt ctttttggat
    14101 aactggcagt tcttctttca cttccacaat cgacaaagct agaacttcta attctgtttc
    14161 ttgacgcttg tttaccgctc gttcgatcaa ttctttgatt ctttgtgctg taccttcgcc
    14221 agatgcgcag atagccaaaa cagctttttt cttatgaata ggttttgctt cctgaacaga
    14281 ttctacagaa gatatttctg cgtaacctcg aaaatttttc aatgattcat gcaacatatc
    14341 tagatctgtc cctaatactg agacctttcg tacagtttcc aaaactagag aagttgttac
    14401 catatcaaca gtacgtactg gaattccagt gtctctttga atttgcgaat taaacgacgc
    14461 aagggacccc atatctacta agagtatgac cccgcttcct tcatctactt cttgtacatt
    14521 gcgttcaatt cggtccaatg ctgttttcgg atccatatcc aaaggcatgt ctactgctcg
    14581 tacgttctct gcatctaaca actgttggac aacttggacc atactgcttg ctgtgctgtt
    14641 tccgtgagct gcaacgacta caccgattct gccgcttgct tgatttgccc gtaacgagac
    14701 gagtagaacg gtcaagtaat agtcttcact ttctggaatc tccacttgaa aatacgtttc
    14761 gatataacta cgcacttctt tcgcaacagc atattcatct ggaaaatcaa aagccatctc
    14821 acggatgtta tcattcgtat gccgttcttc tcctaagttg atttttttca aaaaagaact
    14881 gatatgcaag ctcatagcat aaataaagtt ttgctgaaac tcatatggta aagatctttt
    14941 gaccattaca taaatctgat tggtcacttc tatgattttc ggatctacaa attctgctaa
    15001 tttgctgtct gcattaaaag aaaaaccatg gtctttatag aaagacttca gatggatgtt
    15061 gatatctgta gaaatgaaat gattgattgc ttcttgatcc aaaccatccg acttcagtaa
    15121 agctgcttta tctccgatga tatcatatag attataaggt aactcatagg agtccgattg
    15181 gattttcatg atcgtatcat ttggtgctac agttatcttt ggctccaata ttttagaaag
    15241 ctctgccatt gcactgcggt tgcttgctaa ttgaatcaat ccgctacgaa tcccttctgt
    15301 caaatcatcg attgtgatcg agatttcggg tgattgcata tgatttagaa atcctcgtgc
    15361 acatactaac tggatatttg acttcaattg tcctatattt ccataggtca cactgccgat
    15421 caatgctttt gctacatctt ctgtcaaaga aatctttcgc tgcgtgcggt ttgcttcgtg
    15481 ggcaatcatg atttttacta aatcgacttt ctcatttgca ggacgttttt caaaagccgg
    15541 aagtttgata ttgattggga tacgccgaac aaaagtttct aatagagaag aacctgggtc
    15601 ttctgtggtc gcacctacga tccgtacatt tgcttcatgg gatttcgttg tttccccaag
    15661 acgactgtaa gttccatgat ccataaagta aaagatcatt tcttgacctt caggcggcaa
    15721 ccggtgaatc tcatccaaaa acagcatacc gccatccgct tgatcgataa tccccatttt
    15781 ttcttcatct gcgcctgtaa aagcaccttt tgcgtaccca aataaatgac tcatcaaaag
    15841 ctcagggtta ttggcataat cagcacaatt gaaaacaatc agttccgttt cctcatctat
    15901 tacatgatgc gcttttgcaa aatgatacat cgcgtgtgca aaataagtct ttcctgatcc
    15961 ggtaggtcct gtaatcaaac aatttaatcc tcttggcgga tacaaaatcg cagcttttgc
    16021 ctgttccact gaatttttca tgctgccgtt agctccaatg atttttgcaa agatatcctt
    16081 agtatctatt tggagtgtat ttttttcttc agagaccggc gtttctttgt aactgactac
    16141 atgttttgta actggcttta cgtgcgtaac gagtgtttga tatatgtaac gtactggtcg
    16201 tccgtctgtc tttatcagtt ttccttcgcg cactagctga ttcagatctt tactgctatt
    16261 tgttcgctga atccctaaca cttcagcaat ttcctgtgtt gttactcctt gatcatattc
    16321 tgcctttgtt aaatgagcgg ttttttcttt gacatacata taaattcggt caatgcgttt
    16381 tgccaatggt atcgctcctt ttctatagac tattgtactt aggcttcaaa acataatact
    16441 atgtatttat ttctgtttcg tcaactattt acctgatttt gttttgttga taacacgcgg
    16501 atagatagaa atgttcaatt taaaagaacg aaaaatcccg atacgatttt tataatggaa
    16561 acaagaaaaa gaacactgtt taccatataa ttatttcact gatagcaaac aatatagctt
    16621 ccatcaattg aaaacgatag ttatcaaaac atcctgattc caaattggca aaaaaatatt
    16681 aagtgtaaaa agacaaagta aacttcgtag cctccactac aacttttatt tttataaaca
    16741 aaaaaggctg tgacaaaagt cttgtcacaa cctctttaat cataagattc aattaagctt
    16801 cttgagctac tggataaaca gacacttgtt ttttgttacg gcctttacgt tcgaaacgta
    16861 ctacgccatc aacttttgca aataaagtgt cgtctccacc gatacccacg tttacacctg
    16921 gatagatttt tgtaccgcgt tgacggtaaa gaattgatcc acctgtaact gtttgtccat
    16981 cagcacgttt agcacctaga cgttttgatt ctgagtcacg gccgttagat gtagaaccgc
    17041 cacctttttt gtgggcaaat aattgtagat tcatagataa caacatggtc tgcacctcct
    17101 atttatcatt aattgtttta atttggatat attctgagtt ttcagcttct atcgattgta
    17161 atcctaataa aagattttct aatagaatct cagcaatatt gttttgttct tgtgtcattc
    17221 ctgaagtgac ttcggtataa agataaccgc cttcttcttc attcatttca acaatcggct
    17281 caaaaccggc taatgaggca atcccgttga cagtgctgat cgctaacgca gaaacacccg
    17341 cacaaacgat atcacttcca taaggaccag catcagcatg cccggtcaaa gtgaatgaaa
    17401 cgatctgacc tgcgtcattt cgcttaaatg ttcctttgat catatatagg ccttctttct
    17461 caaacgctat taagcgttga tcgcgtcgat aaccactttt gtatatggtt gacgatgacc
    17521 ttgtttacgg tgtgaatgtt ttttaggttt gtatttgaaa gtaacgactt tcttttgttt
    17581 gccgtgtttt tcaacagtac cttcaacagt tgcacctgca acagttggag cgcctacttt
    17641 tgtagattct ccgcctacta agataacttc gtcaaaaaca actttttctc ctgcttcaac
    17701 gtcaagtttt tcaacataga ttgcttgtcc cacttcaact tttacttgtt taccaccagt
    17761 tttaataatt gcgtacatgc tataaagcac ctccttctga tatacttaga ctcgccatcc
    17821 gaagtgacag catatgctgt gcttattact tgttctgagc ggttgtagct gtggagtcca
    17881 caattacaac agtactagct taccagaaca gcaaccgtta gtcaactaaa tttaaaatca
    17941 aaaagtaaaa caaaaattca agcaatactt ctgcttttct atcctcacag ttctaccaaa
    18001 taaaatagtc gcattgtgtt tcttttattc ttataataaa agaaaaagga ggagcataaa
    18061 tggaataccc aaaagaactg atacaagaag cttctttaag aatgaacggc cgtcctgtag
    18121 aaggatttat cgctgggcaa ggacctcttc atcctaaact gatgctcgtg ggagaagccc
    18181 cagggaaaac agagattgat acgcatgttc cttttagcgg tcaagctgga aaagaattga
    18241 tgcaggcact ttcttcaact ggcttaacac gagaagaggt gtatatcacc agtgctgtaa
    18301 ggagccgccc gtatcgcgtg actcatcgga tcaataaacg gacacaacag tcagaaactg
    18361 tttatccaaa tcggacacct actcgttccg aagtatttgc gcatgcacca atattggatt
    18421 acgaactgat gaaggtccag ccaaaattaa ttgcgacact tggaaacatt ggtcttcaac
    18481 gtttacttgg caaagaatat acggttacca aaaatcatgg tcaactctat acagggcctg
    18541 tcttacaatt aacagaacaa aaggatgcct acgaatggtc agaaaaaaac tatcgaattc
    18601 ttccactttt tcatccagca gctattttct acaatcgctc ccttgtacct gttatccatc
    18661 aagactggca agtactagct tcacttttac agcaatatta aaaagaacag acaagaccat
    18721 aaaatcttca gtttacagtc ttgtctgttc tttttcattg ttttaatggc taattgcagt
    18781 atatttcgca tgtaaagctg tcaacgcatg agcaactgtt tgatgttgat gcgtgaatgt
    18841 atgttcccac aagtgagaaa gttgtttttc tacttctggc tgcaaggaag tatagataat
    18901 cgtttgtcct ctttgctctg ctttttcttg aaattcatca aaaactgcac gtgccgtcaa
    18961 atcaatactt ggtacgcctc gcatggagaa aatgacacca tcgtgatgat ttagttcttc
    19021 caatttattt ttgagttttt ctgaagacat aaaaaacagt ggaccactaa tatacactac
    19081 tgtccagtta ttcagctttt ccgtttctgg cagccccatt ctttgccaat cgatcgtttc
    19141 taatgaaatc gtgatagcag cgcttttgac aacaaaaaat acacagccgc cgatcacccc
    19201 gatcacgata gcaatactta aatcaaagat aactgtacaa atcatcgtaa ggataaacaa
    19261 tagaactgct gaccaatatt tattagtaaa tagctccttg atcgtttccc attcattcat
    19321 acgccaagcc gtcacaatca aaacacctgc aagtgctggc attggaatct gtgacatcac
    19381 tggcgcaaaa acaagcatgg agagcagtaa gacaactgca tgtatcatac cagctaatct
    19441 agtttgtgca ccagatttga tggccacgct tgtacgggcg atggccgcag tagcaggaat
    19501 cccgccaaag aaaggaagca acagatttcc aattccttgc gccattaatt cttgattgct
    19561 atctaagggc ttatttgcca ttcggccagc cgaagcacca catagcaagc tttcaatcat
    19621 tcctaataaa gcaatgctaa tagctggaac tgccacttgt tgcaaggcag aaaagctaaa
    19681 atctcccata ttcaaacgat tactactgat caatgtttgt ggaatcttac ccacagttgc
    19741 tactggtaaa tggaacaata gcataactgc tgttgccaaa atgatagcca gcagcgaact
    19801 aggcattcgt ttcgcccatt tttttggata gataaacatc cctaaaatga ctagccctcc
    19861 catgatcaaa gaggctgtgt ttatcggaaa tcctatcgtt tgataactac ttagtttttc
    19921 aataacgttt gtaccttccg agtgaacacc aaataaatta tcgatctgac ctaatgcaat
    19981 aatgatagca atacctgaag taaaccccgt aattacgggt gctgggataa aagaagtcag
    20041 tgttcctaat cggaaaagtc ctgctgcaag taaaaataga cccgctaaaa aagtagcaag
    20101 caatacacct tgcattccct ttgttgccgc aatagacatc aagatagctg ccatcgcacc
    20161 tgtcggacct gatatttgat aaaaaccgcc tgataaaccg ccaataacga taccggcaat
    20221 gatagccgta atcaaacctg cggccgcatc agctccgctt gataccccaa aagccaaggc
    20281 taaaggcaat gctacagcag ctactgtgat cccagccaaa agatctttct gaaatcttga
    20341 aagatcataa cctgaaaatt ctttttttag tgaggaaaca taatttttga acacctatct
    20401 aactcctttt tcatgaaatt ttctaaaata ccattcatta gaatatcata tttcatgaga
    20461 taaagtaata tttttcacaa agtttcctgt tagataaatt cttatttttt ctatatttat
    20521 aagaaaatag aaagcaatgc caacagagct agcccacctt gtttgagtaa aatccaacga
    20581 tcgctagtca aactgccata taatgcaaca aaaaaaatag atattaatag catacttgtg
    20641 atttctttag atgcggaaga aagataagct ccataaagta gtcccactcc taatagtcca
    20701 ttgtacactc cttggttctt aaacaatgtt tgaacagatt ttctgcttaa ctcttcttta
    20761 ctcattttaa aaaccctact tgtcgtgtca gaatcagttc tcaccgtttc gatatacatg
    20821 atataaaaaa attcaattgc aactagagtc actaaaattc tagatagtat cgtcatatct
    20881 tcttcctctt ttctgatgag ttttattttt ttgctcctta taattgtact cgataaggat
    20941 gtttttttat tttatttgcc tttgaaaaca aacaatattg gcacaaaaaa caaagaaaaa
    21001 ccgttagaaa cctcagtttc taacggtttt ttagcgtcac aggagggatt cgaacccccg
    21061 accgtccgct tagaaggcgg atgctctatc cagctgagct actatgacaa gaatcagcgg
    21121 aacatcaata attatagata attaaattgc ttctgtcaat acgaaatagt tatttaatta
    21181 gcaaataatg aattattttg gcccaactac taaatcaatc ttttaaaacg agacattctc
    21241 cttcagctgt tctaaggtta tcaattgaaa acaggagcta cattgacttc atgctactcc
    21301 tgtttattta gatttcctgt atcctttaat tgacttacaa atatagaaac ttttacatca
    21361 agttcgaatt gagccctcga ttcaatcgat tatctacagg ctgccataat catgtatcac
    21421 aactcgattg tttctgattc ctttgtcatt aagatcacac catcacctaa tggcaagatt
    21481 gtcgatgtca aatcgggatg atccatcaca acacgaagaa attcatttaa tttacgatgg
    21541 atagctcgtt tgccacgggg aatctcttca tctgggagca agatcgttcc tccttggaag
    21601 atgtcatcta ccatcaaaac gccacctttt cttaacaaac gtaagcattc aggtaaaaat
    21661 tcaatatatt ttgattttgc actatccata aaaataaagt catacggacc agacaactcc
    21721 ggcaagatat ctgctgcttg accttcaagc aaagtcactt tatctgtcag tcctagacgt
    21781 tcatagtttt ttttagcttt ctcgatcatg acatcgaaac gatcaattgt cgtaacatga
    21841 gcgttttctc cgattgtcgt ggccatgaca cttgatgaaa aaccgatagc tgctccgatc
    21901 tccaagattc gttcaggctg aagctgtcca agtaaaaatt tcaaaaatac aactgtttca
    21961 tggggaataa ctggtacttc tgctacatga gcttcttctt ccagtttgcc tagctcaccc
    22021 ggtaattttt tttgctgggt acgcataaaa tccaatagtt cttctttgac tacaggacga
    22081 tgcatcattt cattgcgcat actactcctc ctcttcacac attatacgaa caactgacta
    22141 aatctacaag ctgcatgagt gttttctcgt gtttcatgtt cagaagagtc gttggatctc
    22201 tggaatacat ttatgttttt aacattctta caaaaatttc tgtgttcgtc tccgcttctt
    22261 tttcaactct acaaaggtat tccatgctgt gattcctgtt aggcaaaaag caatcgtaaa
    22321 gaataacccc tgaatccatg caaatgccgt ttttgaaagg actagtccaa tatatggaat
    22381 ctgaaagatg ggactaccga taatatttct cttttttact actccaggct caatataagt
    22441 ctttgtatca gcttttgtat aaaaatcttc gtggttgttt tcaacggcta ttacgcgacg
    22501 cgtcgagatt ttttttcctg aattttcata atatgtaata atatctccga ctaaaatctc
    22561 atttgtattt ttcggtcgta catatatcag atttccttgt gcataatatg gactcatttc
    22621 tttgtcctca ataatataag gagtcaaacc aaaaattcgg ggaaccaaga cgacgagaag
    22681 aataagaatt aaaaatataa tcaacgttat agaacttata aaagttattt ttttgtaatc
    22741 aggtatcgtt ttcaacttct ttctttattc aatccttctt aatcatataa taacgataaa
    22801 tttttttcaa attatagc
//
LOCUS       DAZKLD010000033        22789 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.033, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000033 DAZKLD010000000
VERSION     DAZKLD010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22789)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22789)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22789
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.033"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            96..680
                     /locus_tag="ACG8KR_001048"
     CDS             96..680
                     /locus_tag="ACG8KR_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0200436.1"
                     /translation="MDLKQSFYIWLYPDNPMSIMADIFVVSILLYLFYLIFFHNLIGY
                     MILDWKGMFDIERKPFANNQTYTVFESGDWEENEVDVIHEIGLALSGIQDADVLVSMT
                     TDTGYNAYEVFYLMYFIWRDGQEIINGRAEKTINSNTEISFCMGEGEMIVYKDGSANI
                     KTAFGDIETSIKELREYVQNPYNFQYLAGRINLV"
     gene            691..936
                     /locus_tag="ACG8KR_001049"
     CDS             691..936
                     /locus_tag="ACG8KR_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0200437.1"
                     /translation="MKKIINQPTNHLIIEGVIFVTMYFTVGNGISNTIIQKNPQYLLF
                     SLYAWIVQMVWVSILMLLFYWIVRVILLLLNKRKKDR"
     gene            942..1664
                     /locus_tag="ACG8KR_001050"
     CDS             942..1664
                     /locus_tag="ACG8KR_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0200438.1"
                     /translation="MKSTKLKVVLLSTAALITLTACGTTNSANNNKDTKSSSTMVTKN
                     STTSASTTMSSMTENNAEKYQKYEDINKGQKPDEVYNNFINTVKNDENYGNDPLQNKN
                     LTIVLYKPDCPDCQKAESKIMQEIKEGFSYAPTLMHDITINKPAYEYYAVAVDITDGV
                     PEWVQNIPGLADENNQYHTPTVAVVTPKKVNGTLYWDVMGMTTNTTDEMIESTTKYYM
                     DLGAYKLYQQSEPVMDDQSKVK"
     gene            1795..2538
                     /locus_tag="ACG8KR_001051"
     CDS             1795..2538
                     /locus_tag="ACG8KR_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0200439.1"
                     /translation="MKEISANEAHIPQKNQVGIYKVLHGEGIYKIQLLTGYNNGANGK
                     WLKATSIRELRSEVEKIRLGLTSEEIAKLKGNNSFVIEKEKEKHLLYRELKIEEYYEI
                     DLPEKLRIIDQRSEEKWKTEIYPSLNDYKTSGKPMYNYTGFLTYTQYKYAFRKLKGIK
                     QAEIALYEMKKVDRLKLIQDLVQNTWNQAIVELEKENGKILTNKYLIVNEIEELIGLF
                     SCEKGEVFLKSSPTYSILDSPYQFIQARR"
     gene            2544..2909
                     /locus_tag="ACG8KR_001052"
     CDS             2544..2909
                     /locus_tag="ACG8KR_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0200440.1"
                     /translation="MSSLKDDYIQAKQEYEKIQMLKSEKVELFLTQKLAEDHIANLDA
                     KTLEEYDRQFNQIHHNIILLVEQAKRRFEKAEYDLLDQGIVCLDKGLRRYYGAELKKQ
                     NVDRNALKHEILELIMQRI"
     gene            2925..3455
                     /locus_tag="ACG8KR_001053"
     CDS             2925..3455
                     /locus_tag="ACG8KR_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0200441.1"
                     /translation="MSQNISAVKQLIEEDLETTVQRLAERIHFANYQTISNWSSRNRN
                     PEEIPMKFFYRIGKEYGVDPIKIFEKTMIYYYHGTPNEVLEHLLEEAKEKSSILELSQ
                     NDKVIQVKCVDPTGKIIVGFDIERKSKIDFITQLKKINNLDILTLRPDHEQLFMSFKK
                     QLKEIQKRYNKVFPNA"
     gene            3617..3925
                     /locus_tag="ACG8KR_001054"
     CDS             3617..3925
                     /locus_tag="ACG8KR_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0200442.1"
                     /translation="MWNNRLKTGLLLIVISCAMMIGMRIQREQSYFEVSANNVIEKCY
                     YGQHYWSEEVRENIDREYVQRIVWDAYSIKDYPKSLTSRLFYSEKDNQKLSDLMMKKV
                     "
     gene            4056..4340
                     /locus_tag="ACG8KR_001055"
     CDS             4056..4340
                     /locus_tag="ACG8KR_001055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0200443.1"
                     /translation="MTFLNSVFIDYVITTKRIGSYSSTYASLVPMLNSMSRFLKEREK
                     KELQYIKEIMPDFEMHEWQIALSDWKLANKVRRITPFQAKRFLRAMKFVQ"
     gene            4477..5277
                     /locus_tag="ACG8KR_001056"
     CDS             4477..5277
                     /locus_tag="ACG8KR_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0200444.1"
                     /translation="MSEDLRELKLRKKQYDQYFSGLLNKHIEEGLDHDFGKDEYCLYC
                     ERYNEKMSEYLQLKKEIQKLEKKVHAWKYKDSMSGYVESQNKPRKKKQAYVLKMVNGE
                     ELIVLAYSEEEVRELVETPVRKKVETINEIDDSQYNSIYIETSHGGYKKLATFLRSSK
                     VGIISKKDISAVTDSFCTLSVPKAYFQYDSKRFERLEKFCRPYTVLKTKKFNKTKRVS
                     IQLPTGQISLGEKDYLIKVGKELIIKVPEHLFKENFEIRETLVWKEEK"
     gene            5314..5496
                     /locus_tag="ACG8KR_001057"
     CDS             5314..5496
                     /locus_tag="ACG8KR_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0200445.1"
                     /translation="MAVNKILQVLIIDEFGGGEWFDVQSIDFEEEVVVIENIYGEVYS
                     YSLEGENIEDLRLIGE"
     gene            5607..5924
                     /locus_tag="ACG8KR_001058"
     CDS             5607..5924
                     /locus_tag="ACG8KR_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0200446.1"
                     /translation="MKREEYKQRLNELLEEDETLTHGSPDEILYMIDNMVIFGGYELG
                     NRSVDHNILEFDDVSWEEILDWGILAVPETKTYISDTMVPFFEELDYKRLPKNENHIL
                     GGN"
     gene            5927..6070
                     /locus_tag="ACG8KR_001059"
     CDS             5927..6070
                     /locus_tag="ACG8KR_001059"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0200447.1"
                     /translation="MLSDLFILLIYLGVSEIIAYFLFVIANYGISSAIVQMFRLPVSE
                     LKK"
     gene            complement(6274..6753)
                     /locus_tag="ACG8KR_001060"
     CDS             complement(6274..6753)
                     /locus_tag="ACG8KR_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0200448.1"
                     /translation="MTDKTSDLPKLAPKTSRVKSKKDGKTKNAVKKKSVKETSSLLLD
                     IPMSSFDDNTDETSVKIATHVGMNNQNDSFYNLLNSLRELEDKKNQVIASKAPDLTTD
                     LDAEIIQIKQDERSVKSLLKTYFDGFDKELAPYSQAILTKPIQAPHISISLQKKSDD"
     gene            6837..8210
                     /locus_tag="ACG8KR_001061"
     CDS             6837..8210
                     /locus_tag="ACG8KR_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tip lysozyme"
                     /protein_id="HFQ0200449.1"
                     /translation="MKKSKRKIATLLMLMVQFTGIAMSVITINPQVIHADGIATYDAQ
                     FNCTTEGSGSGTDSDGNDTGSGAVTGDVAKNIEDIYKYMNGEYGLSGASIAGILGNWK
                     QESGIDPRAVEGYYGDGQREVDFIKQFTDGLMGIGYGQWTAGRHTALVDWSKKNNGGS
                     WWTTEAQLDFMFKGDGGFVGILKDYALNASDDVVENAVTFHKNWEISADSESAVIAVR
                     GENAKEIWKYMKSKGMDGAKDESKINKISGGSSTGDGSNNSSSSASGTVEDACATTDD
                     ANSGSGSIGKEGKINGKKGQVIANMTYEEALKKYGKAITLPKFKEPDWSTSPFNGIYQ
                     GQCTELTWAYMSQLYDGNQPTTGNGGYVWESYKSAGAKITNKPIVGYGFSASDGWLWA
                     VGDAGHTGVVIAVFEDGSFLCANFNVPPTQAPTRGVSVTLIDGVDGSDNVHFFSGVGK
                     AKFEIEK"
     gene            8233..8760
                     /locus_tag="ACG8KR_001062"
     CDS             8233..8760
                     /locus_tag="ACG8KR_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0200450.1"
                     /translation="MNSKVKDTIIISLLSGMLLALLVGILFFNKQNQDIEAEKHSVHI
                     EDKLTFFKEVKPNEVDGLRKDLENDIQEMLNGSVQNKFADQSGEEFLRKLFIVTGKPP
                     ITDKSSEKEKIEYYKPFTFKLTNFGIKTSSSGLYEALTDFEVQYNGKKVYDTTLTITL
                     NNDKKVTGGAFYAKS"
     gene            8747..9310
                     /locus_tag="ACG8KR_001063"
     CDS             8747..9310
                     /locus_tag="ACG8KR_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0200451.1"
                     /translation="MQNHEITTAKKAIGILSIIVILCCVVLFQKKNAYDNNVKELEDL
                     KSKQIEVVKNNQKVKAMQEAQYKELGNAEIKEQSRKFFNLFYNWNSWEKYTKNMKEIQ
                     KEFPQIVKSSPVDISGTMVGTGNSPISSYTADYYTTNQKGKMVELITQTRNTPTTATT
                     TIWRGVTSIGENQLFYLEDLDSYVRAE"
     gene            9732..10469
                     /locus_tag="ACG8KR_001064"
     CDS             9732..10469
                     /locus_tag="ACG8KR_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0200452.1"
                     /translation="MADHKLEKLLEETWNPKEFSEFFMENFETDLAVIVKDALREQGY
                     PETANYININFTLYTENKGTWDFWATLANKELSDKSDTGIRNFFESNRDDYMYANNQN
                     KLNFRVEFDETPEEFIERQPPKENVAKVLEDRWNSDEIVSTISELGGQYEPLVEAVRE
                     ELRLNKFPDVQNIDVSQIEINVKITNKLDYGSWADIALEKYIYSTLKEFIENRMDIMY
                     LQHPQYLNFGVEIATPLEEWKMEQGLD"
     gene            complement(10486..10902)
                     /locus_tag="ACG8KR_001065"
     CDS             complement(10486..10902)
                     /locus_tag="ACG8KR_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0200453.1"
                     /translation="MNKKRKLSVLITLVSILTILSVILSLKIFNQINTSSNAKDGIVV
                     ENTSNSKETGTTTKKDKSAEITTKNYLIKTVYFDERKTAILKDEEGSELKLPITVSDK
                     FNGTFSLQTAEGSPDNLKVGQTIILGKNDELYSFVK"
     gene            11295..12542
                     /locus_tag="ACG8KR_001066"
     CDS             11295..12542
                     /locus_tag="ACG8KR_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HFQ0200454.1"
                     /translation="MNMKRNSLGMVLLASAVLAGLSVPVTSHADEWTANTPDQIQITT
                     GQSSYAFKKGDTIWSTVEKLKSLGYKTTVAEFAELNNVDLAKGEQRTIPIGRTFNISK
                     DTLTVKEADGEVVSQTVQKDKPDPIENTEQENKKSETTKESEPTANTSDKTETSASSV
                     DGASSSNTNNSTSEKEASQGTSESSTTEADTGTSTNNSQSTSNDANESTSTSSSDQSQ
                     TGSSDETDSSTSPSKPEQPTNVVLGVPFISQGNTMLCEGTSLLEALHYKGVATNQDLM
                     TFVKSMPLSPDNNPNNGFSGEWRHNVDGTYQGMNPAPVVSWGVANGGNVVDISGSSVQ
                     GLKDEIVNGNPVVAWVTYAFEPAQHKPMFWGDAIWNRHVVAVDGFKDGFYHVVDPVFG
                     ASWINAGSFETAYNVSQLAVSVR"
     gene            12810..15200
                     /locus_tag="ACG8KR_001067"
     CDS             12810..15200
                     /locus_tag="ACG8KR_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFQ0200455.1"
                     /translation="MKHEKTIILKSKIIYCFATLVMMLSTLIMPFSAIRAEADVTKPT
                     SAKFIETGVKTDGYIRVNMPNHPNEWMISSQFKDSHGNIGYCMDSELPSPTGSGAGSL
                     KYKGAGSDEFYRMFKGGFPSKTAKELGAGNDTEAWYATQLVSWVLAGNFKVSQIVWSH
                     PNHTAAETARVKKAFEKIYDYAKNGKDTPNTEFSITASKTADEGKYHTFTYKTASNKT
                     GNAKLTFTSAKPAGMKIYDADGKEITNNTVKLNSSFTIKVPVTTPSGTLSFKGTANVS
                     TTNPFTFDGRGVYQDAVVMITTSETKDSKSLSAKWTRAVGQVTVKKVDQDGNALKGAE
                     FTLTDNNNAKSVKTTGDDGLATFDLQQGLTYSLEETKVPEGYQGSFKKDGITVNTDGQ
                     EFSYTATNTIKKGKVKVKKVDQDGKPLANAEFTLTDDSSQTQAEKTDENGLASFDIVA
                     NKTYSLEETGVPTGYTGVFKQEGITVKDDGQTFEYTATNTLKKGLVKVHKVDQDGKPL
                     ANAEFTLTDEKGATQVEKTDENGLASFNIVANKTYSLEETGVPVGYTGAFKQEGITVK
                     DDGQTFEYTATNNLKKGVVKVHKVDQDGKPLADAEFTLTDEKGVKEVKKTDATGLATF
                     NIIANKTYNLEETKVPTGYTGAFKQEGITVKDDGQVFEYTAKNTLKKGVIKVHKVDDQ
                     GKALANAEFTLTDDKGVKVVQKTDSNGLATFNIVANKTYKIEETQNPNGYTGKWSKEG
                     ITVADDGQTFEYTVINHLVPETVRTGGIGTSAFLLVFLIAGLSMVLLYYREQHKAK"
     gene            15249..17084
                     /locus_tag="ACG8KR_001068"
     CDS             15249..17084
                     /locus_tag="ACG8KR_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFQ0200456.1"
                     /translation="MKSKKIFSNLMLFGTLLGVGLGASSISSSLGLGGQVAYAQQNYN
                     YQGTGTIHIHKVTNTNGDGNPVSDIDNKGKEIALPNGVKPLEGVEFSYVKLTKDEAKS
                     LGWTDSDTTKGQHIDDAKARLLLNKKYSEVSRMKTDSQGNIDIPIQVNTDDLTDNLYL
                     IVETGTVKGVVEEAYPMIVNVPELNGSDDQYFNYDVHVYPKNYFQLNSAELDKKDEDG
                     KALAGTVFELRKDDGSDQVVGTYKTDSTGKLTVDGLELGDYYFLETDLGTDANKTTYL
                     LDGKKFKFSVKRGETAKVVLDVTNYKRPVITKVIDKETVDTGEDVQYTLKPSIPGNIG
                     NYEKYVVKDVLGAELTYDSSEVIEGMTLDTDYSIERDGQTTNYVFTESGMDKLQKQFD
                     AGDRFFTILMNAHTNSKAKMDTPLKNDVSTIYNNGYEPEDETPVDHAYTIIGDHQFIK
                     TDGDTSKALSGAKFVVKNKIDPNKVTYLKQDPTTKSVTWIADKSKATEFVSGSDGKFQ
                     IEGLQYTYDVDFDKQGNPTITDKKINDYALEEVVAPSGYTPTRNDVPFVVSNDDASSE
                     VTDIKNLKTPETVRTGGIGTVVFLVVAGVAGTGLYVVYKREQHKA"
     gene            17159..18016
                     /locus_tag="ACG8KR_001069"
     CDS             17159..18016
                     /locus_tag="ACG8KR_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFQ0200457.1"
                     /translation="MKKKNIKLIILKIGMLLCLGVGLYPVITQAYTYYQTQQTLNSYK
                     EKYDEASEKENEEVFKRYSEVNRKRKDTEQTSVTIPEDNNNIPSNGNLQKDKLGELVG
                     EILIPKINVDLPIYDGVNDFQLSAGAGILTGTDYPTGGIGNHSVISAHSGIPNKKYFT
                     DLELLKLGDKFFIDISGKKLAYQVDRIKTILPTEFEDLLPVAGKDYVTLMTCTPVGDN
                     SHRLLVRGVSIPYQKSIVEKEKKKEDLKVKAQMNWYPIVVVGVLLIIAVIVIIKKIVA
                     KRRGMKNEE"
     gene            18006..19115
                     /locus_tag="ACG8KR_001070"
     CDS             18006..19115
                     /locus_tag="ACG8KR_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFQ0200458.1"
                     /translation="MKNKILLFLTLICTLVLTIYPANQLYHQFKTKENVAEIQEKIEK
                     KQTPSKEEVAKKVEQIKSNSGNQGLDNTQKTDKGVDNTQETDGGSFDIREAEGKVNPY
                     TGDVFSKNQTKDFEPSTYSGAVGYVYIPKINVLELLFLGASQEHLYRGVAQMSATSLP
                     LSGKGHQTVIAGHRGGYTSPQFLYVDQLVAGDHIYIDYLGEKAVYTVFRTDVIRNYGQ
                     NSTLTDIPDLDMLTLYTCHPYPQNYDRLLVRSLRDEKAKYITPQVTVGNTAYADAIDS
                     ESVSDKTETVTSSNDLFEDVDRANKELDGNNDLNIPEQGSVEMQDRTFAEKASSIFTG
                     ITNKHQKMLLVIWTVIGALASLKVLILLIKEFRKA"
     gene            19454..20254
                     /locus_tag="ACG8KR_001071"
     CDS             19454..20254
                     /locus_tag="ACG8KR_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0200459.1"
                     /translation="MVGVSKTALDSLAKLFSSRFDGAEFVEYLSQNESGKTLIETMKK
                     DYLNENDFPTWTNIEDATWDVVCTIYSPWVTIADFVLDKIGGDTYPDAKSLATSDFKT
                     FYGYLIGLIGNGIDLKVTPKQTPEEIEKEKGEVQMAYANDREKVKLLEGKLSEEELGK
                     MVQEINEDSNYFPELKVYQNDEDFFNEMFSNSPWEAVSKTNLGEWNFQDDLVAFSNDG
                     YLRSYSESEYRAELKTYETDILMSYLNLLESGQGELRDKVEELLKEEL"
     gene            20360..21148
                     /locus_tag="ACG8KR_001072"
     CDS             20360..21148
                     /locus_tag="ACG8KR_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0200460.1"
                     /translation="MSATTDMALEAVIKDNTGNVENVEDFSDIAINLSAWISAVGEEN
                     IEFEIKNMDDLKAKFPFIASKLSQVAEDKSWNELVSFSEKIYDITQEDECVSWEMNNQ
                     YKKIDAFVKSRIEGTAFEFEGIDTADNWLNKESFIKSIADDLEKQWSKEDYAEYLKTE
                     RDTEVKEKLTEVCLDEHIDPNIVKQEDYEVVIDKVPTDFEEVVKYIVEEALSQYSSDE
                     EVDEETVRTRVWQLVFDDKDYKYLSDQNSVNWSVRLLIDQNDIE"
     gene            complement(21165..22610)
                     /locus_tag="ACG8KR_001073"
     CDS             complement(21165..22610)
                     /locus_tag="ACG8KR_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182 family transposase"
                     /protein_id="HFQ0200461.1"
                     /translation="MLGVILMMRKQSIEGRNQFAMLTIDDLVPKDHLVRKIDAAIQFD
                     FIYPIVESTYSTYGRPSIDPVVLIKLVFIQYLFGIRSMRQTIKDVETNVAYRWFLGYS
                     FEDPIPHFSTFGKNYVRRFRETTLFEDIFSHILEQAVKAGFVTEDNLYIDSTHIKANA
                     NKHKFTKEMTYGQAKAYQDELEDEINKERIAVGKRPFTWDTESELSLQKISHSDPESG
                     YYVKGEREKQFAYSAHTSCEDNGFILSTLITPGNVHDSQVAIDLVKQSKRAFPNIKRV
                     VADAGYKTPKFVHFLSHLKLYPILPYTAPHGVKGMFRKKDFVYDAYFDCYLCPNNQPL
                     LFSTITRDGYRTYKSNPKFCVSCPLLENCTLSTKHQKIVTRHIWADLMDELEHQRHTA
                     LNREIYKKRKQTIERIFADAKEKHGMRWTKYRGLEKVATHTMLTFAAMNLKKLATWLW
                     KTKKPMAFYFKIMQRLTKRSGLITNSFLSTI"
BASE COUNT         8472 a         3194 c         4494 g         6629 t
ORIGIN      
        1 acaagtttac cataaaaaaa aattaaaaag cgagacatat ttatgttgtt gtgttatcat
       61 aataacataa atatgtagag tgagatgatg aaaaaatgga tttgaaacaa tcgttttaca
      121 tctggttata tcctgataat cctatgtcaa taatggctga tatttttgta gttagcatct
      181 tattgtattt attttatcta attttctttc acaatttaat cgggtacatg attcttgatt
      241 ggaaaggaat gttcgatata gaaagaaaac catttgctaa caatcaaacc tataccgtct
      301 tcgaaagtgg agattgggaa gaaaatgaag ttgatgtgat ccatgaaatt ggattagctt
      361 tatcaggaat acaagatgct gatgttttgg tatcaatgac aacggatact ggatacaacg
      421 cttatgaggt attttatctt atgtatttta tatggcgtga tggtcaagaa attatcaatg
      481 gtcgtgctga aaaaacaatc aattctaata ctgaaatttc tttttgtatg ggtgaagggg
      541 aaatgattgt atataaagat gggtcagcaa acattaaaac agcctttggt gatattgaaa
      601 caagtattaa ggaacttagg gaatatgtgc agaacccata taattttcaa tatttagcag
      661 gaagaataaa tttagtttag gagtaaaaat atgaaaaaaa ttataaatca accaacaaat
      721 cacttgataa ttgaaggtgt tattttcgta acaatgtatt ttactgtagg aaatggcatc
      781 tcaaatacaa tcattcaaaa aaatccacaa tacctattat ttagtcttta tgcttggatt
      841 gtacaaatgg tttgggtttc catattgatg ctgttgtttt attggatagt acgggttatt
      901 cttttattgt taaacaaaag aaaaaaggat aggtgaataa aatgaaatca actaaattga
      961 aggttgtatt gttatcgaca gcagcattaa taacccttac agcatgtggt acaactaata
     1021 gtgctaataa taacaaagac acaaagtcta gtagtacgat ggttactaaa aacagcacta
     1081 catcggcatc aacaacaatg tcttcaatga ctgaaaataa tgctgaaaag tatcaaaaat
     1141 acgaagacat taataaaggt caaaagccgg atgaagttta taacaacttt attaatacag
     1201 taaaaaatga tgaaaattat gggaatgatc cattgcaaaa taaaaatctg actatcgttc
     1261 tttacaaacc tgattgtcca gattgtcaaa aagcagaatc aaaaatcatg caagaaataa
     1321 aagaaggatt tagttatgct ccaacattaa tgcatgatat taccataaat aaaccagctt
     1381 acgagtatta cgcagttgct gtagatatta ctgatggtgt acctgagtgg gtacaaaaca
     1441 ttcctggtct ggctgatgag aacaatcaat atcatacgcc aacagttgca gtagtaacac
     1501 caaagaaagt taacggcaca ctatactggg atgtaatggg aatgacaaca aatacaactg
     1561 atgaaatgat tgaaagtaca actaaatatt atatggattt gggagcatat aaattatatc
     1621 aacaatcaga gcctgttatg gacgatcaat ccaaagttaa atagatcttt agggcgtaag
     1681 ccctattttt ttaaaattaa gtgataacaa ataaatgatt tctgaaacaa acgttcttac
     1741 tcaactactc gaaacacaca agctaattaa ctagattata aaggagaatg cgtaatgaaa
     1801 gaaatcagtg caaatgaagc tcatattccg caaaagaatc aggttggtat atataaggtg
     1861 cttcatggtg aaggaattta taaaatacag ttacttaccg gttacaataa tggtgcaaat
     1921 gggaagtggt taaaggcaac aagtattcgt gaacttagaa gtgaagtaga aaaaattagg
     1981 cttggtctca caagtgaaga aatagctaaa ctgaagggaa ataattcttt tgtaattgaa
     2041 aaagaaaagg aaaaacatct actttatcgt gagctgaaaa ttgaagaata ctatgaaatt
     2101 gatttacctg aaaaattaag aattattgat caacgttcag aagaaaaatg gaaaactgaa
     2161 atatatccaa gcttaaatga ctacaagaca tcaggaaagc ccatgtataa ctacacaggc
     2221 tttttaacct acacacaata caaatatgcc tttcgcaaat taaaaggcat taaacaagct
     2281 gaaattgccc tgtatgagat gaaaaaagtg gatagattga aattgattca agatttagtg
     2341 caaaatacat ggaatcaagc gattgtcgag ctagaaaaag aaaatgggaa aattttaact
     2401 aataaatatc tgatcgtaaa tgaaattgaa gaattaattg gtttattttc atgtgaaaaa
     2461 ggtgaagtct tcttgaagtc atcaccaacc tattcaattt tggacagtcc gtatcagttt
     2521 atacaggcaa ggagatgagt tagatgtcta gcttgaaaga tgactatatc caagcaaaac
     2581 aagagtatga aaaaatacag atgttgaaat ctgagaaagt cgaattattt ttaacacaaa
     2641 aattagcaga ggatcatatt gctaacttag atgctaaaac tttagaggaa tatgatcggc
     2701 agtttaatca gatacatcac aatatcattt tgcttgttga gcaggcaaag cgaaggtttg
     2761 aaaaagcgga atatgatcta ttagatcaag gaattgtatg cttggataag ggattgcgaa
     2821 gatattatgg tgcagagtta aaaaagcaaa atgtagatag aaatgcacta aaacatgaaa
     2881 tcttagaatt gataatgcaa cgaatttgaa gggatagaaa gcaaatgtca caaaacatta
     2941 gtgctgtaaa acaattaata gaagaagatt tagagaccac agttcagaga ttagcggaac
     3001 gaatccattt tgcaaattat caaaccatct ctaactggtc aagcaggaac agaaatccgg
     3061 aagaaattcc aatgaaattt ttttatcgaa tcggaaaaga atatggtgtt gatccgataa
     3121 aaattttcga gaagaccatg atttattact atcatggaac acctaacgaa gtgttagaac
     3181 atcttttgga agaggcaaaa gaaaaatcat caatattaga gctatcgcaa aatgacaaag
     3241 ttattcaagt gaaatgcgtt gatccaacag gaaagattat tgtcggtttt gatattgaaa
     3301 gaaaatccaa aatagatttt attacacaat taaaaaagat aaataattta gatattctaa
     3361 cgcttagacc agatcatgaa cagttgttta tgagttttaa aaaacaattg aaagaaatac
     3421 aaaaaagata taacaaggtg tttcctaatg cgtgaaaagc tcaaagaaat cagagttttt
     3481 tggataatta acaaagaatg tatcatctta tatactcttg gatttcttag catgatcttg
     3541 ttttggtggt tatatttaaa acagtaattg gagagggtca agaggatttt tatcaagata
     3601 aggatgtgga aactgcatgt ggaataatag attaaaaaca ggattgttat taatagttat
     3661 ttcctgcgct atgatgattg gtatgaggat acaaagggaa caatcctatt ttgaagtttc
     3721 tgctaacaac gtaatagaaa aatgctatta cggtcaacat tattggagtg aggaagtcag
     3781 agaaaatata gatcgtgaat atgttcaaag aattgtttgg gatgcatatt ctatcaaaga
     3841 ttatcctaaa agtttaacga gtagattatt ttacagtgaa aaagataatc aaaaactctc
     3901 agatttgatg atgaaaaaag tatgactatt attacgaaag agaaacagag tgctatattc
     3961 gaggggaaaa agcctacaac aaaagtgact attggggtga caaggcgaag cgattaactg
     4021 caaattttag agtgtatgaa tcagagttta taaacttgac ctttcttaat agtgttttta
     4081 ttgattatgt aattactacg aaacgcattg gaagttattc aagtacctac gcatccttag
     4141 ttccaatgtt aaactcgatg agccgtttct taaaagaaag agaaaagaaa gagctacagt
     4201 atatcaaaga aatcatgccg gattttgaaa tgcacgaatg gcaaattgct ttatcagatt
     4261 ggaaattggc gaataaggtt cgtagaataa caccttttca agccaagcgc tttttgagag
     4321 ctatgaaatt tgtgcaataa aaaaagccac cagacaagtt gatggagact aacacaatta
     4381 ccaacagttg taattgtgtt agcttacaaa ttaattatgg tgtttagtca atcccaaatt
     4441 gtgaaatggc accaataatt ggaaaggaaa aatgaaatgt ctgaagattt acgagagctg
     4501 aagttacgaa agaagcagta tgatcagtat ttttccggtt tgttgaataa acatattgaa
     4561 gaaggacttg atcatgattt tggaaaagat gaatattgtt tatattgcga acgttacaat
     4621 gaaaaaatgt ctgaatatct acagctgaaa aaagagatac agaaattaga gaaaaaagta
     4681 catgcttgga agtataaaga ttccatgtct ggttatgttg aatctcaaaa taagccaaga
     4741 aaaaagaagc aagcgtatgt tctaaagatg gtaaatgggg aagagttaat tgtattagcg
     4801 tacagtgaag aagaggttcg agaactcgtt gaaacacctg tcaggaaaaa ggttgaaaca
     4861 ataaatgaga tagacgacag ccaatacaat agcatctata ttgaaacaag tcatggtgga
     4921 tataagaaat tagcaacttt ccttagaagt tctaaagttg ggataatttc taaaaaagac
     4981 ataagtgctg ttaccgatag tttttgtact ttatctgttc ctaaagctta tttccaatac
     5041 gatagtaaaa gatttgaacg cttagagaaa ttctgtagac cgtatactgt attgaaaact
     5101 aaaaaattta ataaaactaa aagagtttct atacagttac cgactggtca aatctcatta
     5161 ggtgaaaaag actatctgat aaaagtagga aaagagttaa ttataaaagt accggaacac
     5221 ttatttaaag aaaattttga gatacgtgaa accttggtat ggaaagaaga aaaatagttt
     5281 ggaagacata gaatggtata aggggaaatt gaaatggcag taaataaaat attgcaggta
     5341 ctaatcatag acgaatttgg cggaggagaa tggtttgatg tgcagtccat agactttgaa
     5401 gaagaagtcg tagttataga aaatatttac ggcgaagtat attcatattc gcttgaaggt
     5461 gagaatattg aggatttacg gttgattggt gagtagaaaa aaacagaaat atcaaaatgg
     5521 agataaaaat taatagcatg taaagatagt catgcttggg ttaaaaatgt taaaattcaa
     5581 aaaaatgagt cagaagggat aaaagtatga aaagagagga atacaaacaa agattaaacg
     5641 aactgcttga agaggacgaa acattaactc atggttcgcc tgatgaaatt ctatacatga
     5701 ttgataatat ggtgatcttt ggtggatatg aacttggtaa taggtcagtt gatcataata
     5761 ttttagaatt tgatgatgta tcatgggaag aaattcttga ttggggaatt ttggcggtac
     5821 ctgaaactaa aacctatatt agtgacacta tggttccttt ttttgaggaa ttggattata
     5881 aaagacttcc caaaaatgaa aatcatatac tgggaggaaa ctaacaatgt taagtgactt
     5941 atttatttta ctgatctatc taggtgtaag tgaaattatt gcgtattttt tgtttgtcat
     6001 tgctaattat ggtatttcca gtgcaatcgt tcaaatgttt agattaccag taagtgaact
     6061 aaaaaaatga tgagattatc agcatatctt ttaatgtttt tgaatgtggt cacgttaatc
     6121 tttgtagtaa tcggtgtgac tttcgattta atggaatagt gggaaatcta taatggaaaa
     6181 aagagacacg aatgaacata ttttttgaat caatattgtt gatggttttt gcgatcagcg
     6241 cttaccagaa atctaaaaaa gagtaatgat tgtttaatca tcactctttt tttgtaggga
     6301 aattgaaata tgtggagctt gtattggttt agttaggatt gcttggctat aaggagctag
     6361 ttctttatca aaaccatcaa agtaggtttt cagtagcgat ttaactgatc gctcatcttg
     6421 tttgatttgg ataatttcag catccaaatc ggttgtgaga tcaggagctt ttgatgcgat
     6481 tacctgattt tttttatctt ccaactctct taggctgttt aacaagttgt aaaaagaatc
     6541 attttgattg ttcattccaa cgtgagttgc aattttaaca gaagtttcgt ccgtattgtc
     6601 atcaaaagaa ctcattggaa tatcaagtag taacgaggat gtttctttta ctgacttttt
     6661 ctttacagca ttttttgttt ttccatcttt tttgctcttt actcgagatg tttttggagc
     6721 tagtttaggt aaatcacttg ttttatcagt catttattag cttttccttt ctattaattt
     6781 atgttattat attatcataa taacataaaa aagtacactt tttgggggcg tagttattga
     6841 aaaaatctaa gagaaagatt gccacattgc ttatgttaat ggtgcaattt acagggattg
     6901 caatgagtgt aatcacaata aatccgcaag tgatacatgc tgatgggata gctacttatg
     6961 atgcacaatt taactgtact actgaaggta gtgggtcagg aacagatagc gatggaaacg
     7021 atactggaag tggcgctgta acaggtgatg tagccaaaaa tattgaagat atttacaaat
     7081 acatgaatgg agaatatggc ttatcaggtg ctagtattgc aggtatttta ggtaactgga
     7141 aacaagaatc aggtattgat cctcgagcag tagaagggta ttacggagat ggacagagag
     7201 aagttgactt catcaaacaa tttactgatg gtctgatggg tattggctat ggtcaatgga
     7261 cagctgggcg acatacagca ttagttgatt ggtctaagaa aaataatggc ggttcttggt
     7321 ggactacaga agcccaattg gactttatgt tcaaaggtga tgggggattc gtaggaatat
     7381 taaaagatta tgctttaaat gcttctgatg atgtcgtaga aaatgcagta acttttcata
     7441 aaaactggga aatttcagct gattccgaat cagctgtaat agctgtacgt ggtgaaaatg
     7501 caaaagaaat ctggaagtac atgaaaagca agggtatgga cggtgcaaaa gatgaaagta
     7561 agatcaataa aatatccgga ggttcaagta ccggagatgg atcgaataat tcatcaagtt
     7621 cagcatcagg aacagtagaa gatgcctgtg caactacgga tgacgctaat tctggttctg
     7681 gatcaattgg gaaagaagga aaaattaacg gtaaaaaagg tcaagtaata gcgaatatga
     7741 cttatgaaga agctttgaaa aaatatggga aagcgatcac gctaccaaaa ttcaaagaac
     7801 cagattggtc aacttcacct tttaacggaa tatatcaagg tcagtgtaca gagttgacgt
     7861 gggcttatat gagccaatta tacgatggga atcaaccgac tactggaaat ggtggttatg
     7921 tatgggaaag ctacaaatcg gcaggagcaa aaataacgaa caaaccaatc gtaggttatg
     7981 ggtttagtgc ttcggatgga tggttatggg ctgttggaga tgcagggcat actggtgtag
     8041 ttatagcggt gtttgaagat ggatcatttc tatgtgctaa ctttaatgtt ccaccaaccc
     8101 aagctccaac aaggggagtt tcggtaacgt tgattgatgg agtggacgga tcagataatg
     8161 tgcatttctt tagcggtgta ggaaaagcta aatttgaaat cgaaaaataa attggaatga
     8221 aggacgatat agatgaatag taaagtgaaa gatacaatta tcatttcttt gctttctggt
     8281 atgttattag cactgcttgt tggaatccta ttcttcaata agcagaatca ggacatagaa
     8341 gctgaaaaac acagtgtgca tattgaagat aagctgacat tctttaaaga ggttaaacca
     8401 aatgaggtgg atggattacg gaaagattta gaaaatgata ttcaagaaat gttgaatgga
     8461 tcagttcaaa ataagtttgc agatcaatca ggtgaagaat ttttgagaaa actatttatt
     8521 gtaacaggta aaccaccaat tactgataag agtagtgaga aagaaaaaat cgagtactat
     8581 aagcctttta cttttaagct aacaaacttt ggaattaaga catctagtag tgggttgtat
     8641 gaagcattga ccgattttga agttcaatac aatggaaaaa aagtgtatga taccacatta
     8701 accatcacac taaataatga taagaaagtg actgggggtg ctttttatgc aaaatcatga
     8761 aatcactact gctaaaaagg cgatcggaat actttccata attgtgatcc tttgttgtgt
     8821 ggtactattt caaaagaaaa atgcttatga taataatgtc aaagaattgg aagaccttaa
     8881 aagtaaacaa atcgaagtag tcaagaataa ccagaaagtg aaagctatgc aggaagctca
     8941 atacaaagaa ttgggaaatg ctgaaattaa ggaacaaagt aggaaattct ttaatttatt
     9001 ttacaactgg aattcatggg aaaaatatac gaagaatatg aaggaaatac aaaaagaatt
     9061 tcctcagata gtaaagtcta gtcctgttga tatttctggc acgatggtag gcactggtaa
     9121 tagtccaata tctagctata ctgctgatta ctatacaaca aatcaaaaag gtaaaatggt
     9181 tgaattgatt acacagacaa gaaatactcc aacaacagca acaacaacaa tttggcgtgg
     9241 agtaacaagc ataggagaaa atcaattgtt ttatttggag gatttagata gctatgtaag
     9301 agcagagtaa aataaaaaaa gagctaacgg taccaacgtt agctctttgt gctgacgatg
     9361 ataacaaaac ctaccgaaaa aggattgttt catcacaaac acaaacagaa aagaattgat
     9421 aaaaaacccg aacctcagaa tttttattaa tcaattcatt tagattataa taaattgagc
     9481 tgaacattac aatacgtata tgttgtaaaa cgtaaaaaaa gtcaaaagta aatactttct
     9541 taaaaagtca aataaaacta ttaaagacaa tatatatggt ttatttgaca taagaaaatg
     9601 gtctagatga taattgtaat atagaattgt agacttaaat aaagcataaa atacctttta
     9661 tgataaggta tagctcttaa ttttaagcag ttttagatat aagattgaaa gtaaagtggg
     9721 ggagaataat aatggcagat cataaattag aaaagttatt agaagaaacg tggaatccaa
     9781 aagaatttag tgaatttttt atggagaatt ttgaaactga cttagcagtg attgttaaag
     9841 atgctcttag agagcaaggg tatcctgaaa ctgcaaacta tataaacatt aattttactt
     9901 tgtatactga aaataaagga acttgggatt tttgggctac tttagcaaat aaggagttaa
     9961 gtgataaatc agacacagga attcgtaact tttttgaatc aaacagagat gattatatgt
    10021 atgctaacaa tcaaaataaa ttgaacttta gagtagagtt tgatgaaaca cctgaagaat
    10081 ttatagaaag acaaccgcct aaagaaaatg tagcaaaggt attggaagat cgttggaatt
    10141 cagatgaaat agtttcaaca attagcgaac taggcggaca atatgaaccg ctagtagaag
    10201 cggttagaga agagctaaga ctgaataagt ttcctgatgt tcaaaatatt gatgtttctc
    10261 aaattgaaat aaacgtcaag atcacgaata aacttgatta tggtagttgg gcagatatag
    10321 cattagaaaa atacatctac tcaacgttaa aagagtttat cgaaaataga atggatatca
    10381 tgtatctaca gcatccacag tatttaaact ttggtgttga aatagctaca cctcttgaag
    10441 aatggaaaat ggagcaaggg cttgactagc ctttgctcca tttttttatt ttacaaatga
    10501 atacagttcg tcgtttttac caagaatgat tgtttgccca actttgagat tgtcaggtga
    10561 tccttcagcg gtttgtaagg agaacgttcc attgaactta tcggataccg ttataggtag
    10621 ctttaattct gatccttcct catctttgag aattgctgtt tttcgttcat caaaataaac
    10681 agtctttatt aaataatttt tcgtagtgat ctcagcagat ttgtcttttt ttgtagtagt
    10741 accagtttct ttagaattag aggtattttc tacgactatt ccatctttgg catttgagct
    10801 tgtgtttatt tgattgaata ttttcagcga caagataacg ctaagaatag tcaatataga
    10861 aacaagtgta attaatactg ataatttcct ttttttgttc attttttctt aacatccttt
    10921 tttgttttga ttagttagat attccttatc taacaagcct ttttaggcta ttaaatgaag
    10981 ataaacccca aaaaaatatt gcaattatga cagtgcaatg gtactattat tatacaataa
    11041 attttgcgaa aggggtaggt ctaaatcgca aacaaaaaga aattgaacct agacaaatac
    11101 aacttgtata ccgcaaggga agcaagtttg attttgggta aaggtagaaa ttatgttaca
    11161 aacataatca actattatcc aaatcgtttg cctaaaggtt cgttcaagaa agtgggtgac
    11221 aaaacctata ttataacgga agaaggaata gaagcattgc gatcacaaac agaaaataaa
    11281 aacaaaaaga aggaatgaat atgaagagaa attcgcttgg tatggtttta ttggcatcag
    11341 ctgtacttgc ggggttatca gtaccagtaa cctcacatgc tgatgaatgg actgcaaaca
    11401 caccggatca aatccaaatt acaacaggac aatcaagtta tgcgttcaaa aaaggagata
    11461 ctatttggtc aactgtagag aagcttaaaa gcttaggtta caagactact gttgctgagt
    11521 ttgccgaatt gaacaatgtt gatttggcaa aaggagaaca acggactatc ccgattggac
    11581 gaactttcaa catcagtaaa gatacattaa cagttaagga agcagatggc gaagtagtta
    11641 gtcaaactgt tcaaaaagat aaacctgatc ctattgaaaa tacggagcaa gagaacaaaa
    11701 aatccgagac tacaaaagaa tcggaaccaa cagcaaacac ttctgataag acagaaacaa
    11761 gtgcttcaag tgttgatgga gcaagttcat ctaatacaaa taattctaca agtgagaaag
    11821 aagcatcaca gggaacaagt gaaagtagca ctacggaagc tgatactgga acatcaacaa
    11881 ataactcaca atcaacatca aatgatgcaa atgaatcaac cagtacatct tctagtgatc
    11941 aatcacaaac aggttcgagt gatgaaactg acagttctac ttcaccaagt aaacccgaac
    12001 aaccaactaa tgttgtgcta ggcgttccat ttattagtca aggtaacaca atgctttgcg
    12061 aaggaacatc tttgctagaa gcgttacatt ataaaggtgt tgcaacaaat caagatttaa
    12121 tgacttttgt taaatcaatg ccactatctc ctgacaataa tccaaataat ggtttttctg
    12181 gtgaatggag acacaatgtg gatggaactt atcaaggtat gaatccggct cctgtagttt
    12241 catggggtgt tgctaatggc ggaaatgttg tagatatttc tggttcatcg gtacaaggat
    12301 taaaagatga gattgtaaat ggaaatcctg tagtagcgtg ggtaacttat gcctttgaac
    12361 ctgctcaaca taaaccaatg ttttggggcg atgctatttg gaatcgacat gtagtagccg
    12421 ttgatggatt taaagacggt ttttaccatg ttgtcgatcc tgtgtttggg gcaagttgga
    12481 ttaatgcagg atcatttgaa acagcatata acgtatctca attagcggtt tcggtacgtt
    12541 aataaaagat atcgtttata tactaagtag ttatatagtc tttatttgtc cttgcttaac
    12601 tacttagaaa acttgttgct catggtgggt agaactaaaa ggggtgatgc tatgaatact
    12661 agagcgttga aacagcttgc tttcaacgaa aaaaatgatt aataggtgtg gtgattttaa
    12721 cttaggaatg ggcgcgaagg tcattaccac accgacatat cgtggctcat tttcttcgcc
    12781 taactggtat aaagaaggag taacgagaga tgaagcatga aaaaacgata attttaaaat
    12841 caaaaataat ttattgtttt gcgactttag taatgatgtt atctacgctt ataatgcctt
    12901 tttcagcaat aagagctgaa gcagatgtca caaaaccgac aagtgcaaaa tttatagaaa
    12961 ctggcgtaaa aacagacggt tatattaggg ttaatatgcc gaatcatcca aatgaatgga
    13021 tgatttcaag tcaatttaaa gattctcatg gaaatattgg atattgtatg gattcagaat
    13081 tgccatcccc aacaggaagt ggagccggaa gcttgaagta caagggagct ggcagtgatg
    13141 agttctatcg tatgtttaaa ggtggttttc catcaaaaac agcaaaggaa ctgggtgcag
    13201 gaaatgatac agaagcatgg tatgcaacac aattagtttc atgggtttta gccggaaact
    13261 ttaaagtttc acagattgta tggtcacatc caaaccatac cgccgcagaa acagctcgtg
    13321 taaaaaaagc atttgaaaaa atttatgact atgctaagaa tggaaaagat acgcccaaca
    13381 ctgagttttc tatcacagct tctaagacag ctgacgaagg aaaatatcac actttcacat
    13441 ataaaacagc atcaaataag actggtaatg cgaagctcac ttttacatca gcaaaaccag
    13501 ctggtatgaa gatttatgat gcagatggta aagaaattac taacaacact gtgaaattaa
    13561 attcaagttt tactattaaa gtacctgtga ctacaccaag tggtactttg tcatttaaag
    13621 gtactgctaa tgttagcaca accaatcctt ttacttttga tggacgaggt gtgtatcaag
    13681 atgccgtagt tatgatcact acttcagaaa caaaggactc taaatctttg agtgctaaat
    13741 ggacaagagc tgttggtcag gtaacagtta agaaagttga tcaagatgga aatgctttaa
    13801 aaggagcgga gtttactcta actgacaata acaatgctaa atctgttaag acaaccggag
    13861 atgatgggtt agctacgttt gatttgcaac aaggcttaac atacagctta gaagaaacaa
    13921 aagttcctga aggttatcaa ggttcattta aaaaggatgg tattactgtt aataccgatg
    13981 gacaagaatt tagttacact gctacgaaca caattaaaaa aggtaaagta aaagttaaaa
    14041 aggttgacca agatggaaaa ccattagcta atgctgaatt taccttgacg gatgattcat
    14101 cccaaacaca agcagaaaaa acagatgaaa acggacttgc aagcttcgat attgtagcaa
    14161 acaagaccta cagccttgaa gagaccggag taccaactgg ttatacaggc gtattcaagc
    14221 aagaaggaat taccgttaaa gatgatggac aaacttttga atatacagct acaaacactt
    14281 tgaaaaaagg tttagtaaaa gttcataaag tcgatcaaga tggaaaacct ttggcaaatg
    14341 cagagttcac tttaaccgat gaaaaaggtg caacacaagt agaaaaaaca gatgaaaatg
    14401 gacttgcaag cttcaatatt gtagcaaaca agacgtatag cctagaagaa accggagtac
    14461 cagtaggtta cacaggtgca tttaagcaag agggtatcac cgttaaagac gatgggcaaa
    14521 cttttgaata tacagctaca aataatttga aaaaaggtgt agtaaaagtt cataaagtcg
    14581 atcaagatgg aaaaccttta gcagatgcag aattcacctt aacggatgaa aaaggtgtaa
    14641 aagaagttaa aaagacggat gcaactggtc tggctacgtt caacattatt gcgaacaaaa
    14701 cttataactt agaagaaaca aaagtaccaa caggttacac aggcgcattc aaacaagaag
    14761 gaattaccgt caaagatgat ggacaagtct ttgaatatac tgctaaaaac actttgaaaa
    14821 aaggtgtgat caaagttcat aaagtggatg atcaagggaa agcattagca aacgcagaat
    14881 tcactttgac agacgacaaa ggtgtaaaag ttgtacaaaa aacggacagc aatggcttgg
    14941 caacatttaa tattgttgcc aacaaaacgt acaaaatcga agaaacacaa aaccctaatg
    15001 gttatacagg gaaatggtct aaagaaggca tcaccgttgc tgacgatgga caaacctttg
    15061 aatatacagt aattaatcac cttgtacctg aaacagtaag aacaggcgga atcggtacat
    15121 cagccttcct tcttgtattt ctaattgcag gattgagtat ggtgctactg tattatcgag
    15181 aacagcacaa agcaaaataa aaaagtgtta atggaaaaga agtaaatatg aaaaaagaag
    15241 gctgacgtat gaaatcaaaa aaaatttttt caaatttaat gctatttggt actttgctag
    15301 gagtgggatt gggcgcaagc tcaatctctt ctagccttgg actagggggg caagtagcat
    15361 atgcacaaca aaactataat tatcaaggaa ctggaacgat tcatatccat aaagtaacaa
    15421 atactaatgg ggatgggaac cctgtctcag atattgataa caaagggaaa gaaatagctt
    15481 taccaaatgg tgtaaaacct cttgaaggcg ttgagttctc ttatgtgaaa cttactaaag
    15541 atgaagctaa atccttggga tggacggata gtgatactac aaaaggtcaa catattgatg
    15601 atgctaaagc acgattatta ctaaacaaaa aatacagtga agtaagtaga atgaagactg
    15661 attcacaagg gaatatagac atacctatac aagtaaatac agacgattta acagataact
    15721 tgtatttgat tgttgaaaca ggaacagtca aaggtgtagt tgaagaagcg tatcctatga
    15781 ttgttaacgt acctgaacta aacggatcag atgatcaata ctttaattat gatgtacatg
    15841 tctatcctaa aaactatttc caattaaact cagcagagct tgataaaaaa gacgaagatg
    15901 gaaaggctct tgccggaact gtttttgaat tgcgaaaaga tgatggatct gatcaggttg
    15961 ttggaactta taaaacagac agcacaggta aattaactgt agatggtttg gaattaggtg
    16021 actattattt cctagaaacc gatttgggaa cagatgctaa taaaacaacc tatttactag
    16081 atggtaaaaa attcaaattt agcgttaaac gtggggagac tgctaaagta gttcttgatg
    16141 ttaccaacta caaacgacct gtaattacta aggtaattga taaagaaacc gttgatactg
    16201 gcgaagatgt tcaatataca ttgaaaccat caattcctgg taatattggt aattacgaaa
    16261 aatatgttgt caaagatgtt ctaggtgcag agttaactta tgattcatca gaagtgatcg
    16321 aaggaatgac tttagatact gattacagta tcgaacgtga cggacaaacg acaaactatg
    16381 tctttactga atcaggtatg gataagcttc agaaacaatt tgatgcaggg gatcgcttct
    16441 ttacaatttt gatgaacgct catactaata gtaaagctaa aatggataca ccacttaaga
    16501 atgatgtttc aacgatctac aacaatggat atgaacccga agacgaaaca ccagttgatc
    16561 acgcttatac aatcattggt gatcaccaat tcattaaaac agatggagat acaagcaaag
    16621 cattgagtgg agcaaaattt gtagtgaaga acaaaattga tccgaataaa gttacttacc
    16681 taaaacaaga tccaacaacg aaatctgtta cttggattgc agataaatct aaagctactg
    16741 aattcgtttc cggatcagat ggtaagttcc aaattgaagg cttgcaatat acctatgatg
    16801 tagattttga taaacaaggt aatccaacaa tcacagataa aaaaatcaat gattatgctc
    16861 ttgaagaagt tgttgctcct tcaggatata caccaactag aaatgatgtt ccatttgtgg
    16921 tatcgaatga tgatgcttct agcgaagtaa cagacatcaa gaaccttaaa acacctgaaa
    16981 cagttcgtac aggtggaatt ggaacagtag tatttctagt ggttgcaggt gtggcaggaa
    17041 cagggctata tgttgtctac aaacgagaac aacacaaagc ttaatgaaat aaaattccct
    17101 agagtgtttt tggctctagg gtttcttttt aaaaagtgtg tgaaaagtag gtatgagcat
    17161 gaaaaagaag aatatcaagt tgatcatctt aaaaattggg atgttgctat gtttaggagt
    17221 aggtctatat cctgtaatta ctcaggctta tacctactac caaactcaac aaacactaaa
    17281 ctcctataag gaaaaatatg atgaagcttc tgaaaaggag aatgaagaag tcttcaagag
    17341 atattcagaa gttaatagga aacgaaaaga cacagaacaa acctcagtca cgattcctga
    17401 agataataac aatattccgt caaatggaaa tcttcagaag gataaattag gtgaattagt
    17461 gggtgaaatt cttataccaa agattaatgt ggatttgcca atttatgatg gtgtaaacga
    17521 tttccaacta tcagcaggag caggaatatt aaccggaact gattatccaa ctggtgggat
    17581 tggaaaccat tctgttatat cagctcattc aggcattcct aataaaaaat attttacaga
    17641 tttagagttg ctcaaacttg gtgataaatt ttttattgat atttcaggga aaaaacttgc
    17701 ttatcaagta gatcggataa aaacaattct accaaccgaa tttgaggatt tgcttcctgt
    17761 ggcaggaaaa gattatgtaa cacttatgac atgtacacct gttggagata attctcatag
    17821 gttactcgtt agaggggtta gcatacctta ccaaaaaagt attgttgaga aagaaaagaa
    17881 gaaagaagat ttaaaggtaa aggcgcagat gaactggtat cctatcgtag ttgtaggggt
    17941 tttattaata atagctgtga tagtcattat taaaaaaata gtagcaaaga gacgaggaat
    18001 gaaaaatgaa gaataaaata ctactttttt tgactcttat ttgcactttg gttttaacaa
    18061 tttatcctgc aaatcagctc tatcatcaat tcaaaacaaa agagaatgtg gcggaaatcc
    18121 aagagaaaat tgagaaaaaa caaactcctt caaaagagga agtggcaaaa aaagtcgaac
    18181 aaataaaatc gaacagcggt aaccaaggat tagacaatac tcagaaaaca gataaaggag
    18241 tagacaatac tcaggaaaca gacggtggat catttgatat tcgtgaagct gaaggaaagg
    18301 tcaatccata cacaggagat gttttttcaa aaaatcaaac gaaagatttt gaaccatcta
    18361 cctattcagg agcggttggt tatgtctata ttccaaaaat aaatgtgcta gagcttttgt
    18421 ttctaggcgc ttcacaggag catttatata gaggtgtggc acaaatgagt gctactagtc
    18481 tacctttatc aggtaaaggt catcaaacag taatagcagg tcatcgtggc ggttatacgt
    18541 caccacaatt tctttatgtt gatcaattag tagccggaga ccacatttat atagattatc
    18601 ttggtgaaaa agcagtttat acggttttcc gaacagacgt tattagaaat tacggtcaga
    18661 attcaacact gactgatatt cctgatttag atatgttaac actttatact tgtcatccat
    18721 atcctcagaa ctacgatcgt ttattagtta gaagtctaag agatgaaaaa gcaaaataca
    18781 tcacgccaca agtaacagtt ggcaataccg catacgctga tgccattgat agcgagtccg
    18841 tttcagataa gacagaaact gttacatctt ctaatgattt atttgaagat gttgatcgtg
    18901 ccaacaagga attagatgga aataatgatc ttaatattcc ggaacaaggt agcgtagaga
    18961 tgcaagacag aacgtttgct gaaaaggcaa gttcaatatt tacaggaatt accaataaac
    19021 atcagaaaat gcttttagtc atttggactg taataggagc attagcaagt ctaaaggttc
    19081 ttattctttt aatcaaagag tttcgaaaag cataatgaag attataaaaa ttgatagagt
    19141 acagtaaaaa gggattgata ctattattga tctctttttt attttgaata gagctagatt
    19201 cacttttaaa taaaatcaaa actgatctta attctaaaga aaaagtcaag aaaaaaatac
    19261 aataaatttt tttatcagaa tatttatcag aatattatta gaaaagataa actcaaaagc
    19321 ttaatttaat agggttattg gttgtattta gaaataatgt atggaaaagc tataaagcaa
    19381 tagataagat gtagaaaatc ttatttgtga atgaaaaaca agtcatcgta aaatattaat
    19441 aggaggtgta gtaatggtgg gagtatcaaa aacagcttta gactcattag ctaaactatt
    19501 tagtagcagg tttgatggtg cagaatttgt tgaatatcta agccaaaacg aaagtggaaa
    19561 aactctgatc gaaacaatga aaaaagacta tttgaatgaa aatgactttc cgacatggac
    19621 gaatattgaa gatgctacat gggatgtagt atgtacgata tattctcctt gggtaacgat
    19681 tgctgatttt gttttagata aaattggagg ggatacatac cctgatgcca aatctcttgc
    19741 tacaagtgac tttaaaacgt tctatggtta tctaataggt ctaataggca atggaataga
    19801 tttgaaagtt acgccaaaac aaacacctga agaaattgaa aaagaaaaag gagaggttca
    19861 aatggcttat gcaaatgaca gagaaaaagt aaaattattg gaaggtaaat tatctgaaga
    19921 agaacttgga aaaatggtgc aagaaatcaa tgaagacagc aactattttc ctgaattgaa
    19981 ggtatatcaa aatgacgaag atttctttaa tgaaatgttt agtaatagtc catgggaagc
    20041 agtaagtaaa acaaatttag gagaatggaa ctttcaagat gatctagttg catttagcaa
    20101 tgatgggtat ttacgatcat attcagaaag tgagtataga gctgaattaa aaacgtatga
    20161 aacggatatt ctgatgagct atttaaatct attagagagt ggtcagggag agttaagaga
    20221 taaagttgaa gaactattaa aagaagagct atagggatta tttcaacata aacacaagca
    20281 gaaaactacc aaacaaaacg taccatgcaa aaagtcggtt aaaccggctt tttgctctat
    20341 tatgagagga gatcaaacca tgagtgcaac aaccgatatg gctttagaag cagtaataaa
    20401 agataataca ggaaatgtcg aaaatgtaga agactttagc gatattgcaa tcaatctttc
    20461 agcatggatt tcagctgtag gagaagaaaa tatcgaattt gagatcaaga atatggatga
    20521 cttaaaagca aaatttcctt ttattgcatc gaaactaagt caagttgccg aggataaatc
    20581 gtggaatgaa ttagttagtt tttccgaaaa aatttatgat attacgcaag aagatgagtg
    20641 tgtttcatgg gaaatgaata atcaatacaa gaaaattgac gctttcgtca aatcacgtat
    20701 agaaggaaca gcttttgagt ttgaaggtat agataccgca gataattggt taaataaaga
    20761 atcctttatt aaaagtatag cggatgatct tgaaaaacaa tggtctaagg aagactatgc
    20821 agagtaccta aaaacagaaa gagatacaga agtgaaagaa aaactcactg aggtatgttt
    20881 ggatgaacat attgatccca atatcgtaaa acaagaagac tatgaagtgg ttattgataa
    20941 agttcctact gattttgagg aagttgtaaa gtatatagtt gaagaagcat tatcgcaata
    21001 ctcttcagat gaggaagtgg acgaagaaac tgttcgtacc agagtttggc aactggtttt
    21061 tgatgacaaa gattataaat atttgagtga tcaaaactcg gttaattgga gtgtacgatt
    21121 attaattgat caaaatgata ttgaatagag ctgaaaagct ctattcagat tgtagacaaa
    21181 aaggagttgg taattaagcc actccttttt gttagtcttt gcataatttt gaaataaaaa
    21241 gccataggct tttttgtttt ccatagccat gtggctagtt tttttaaatt catggcagca
    21301 aacgtaagca tcgtgtgcgt ggcgactttt tcaagacctc gatatttcgt ccaacgcata
    21361 ccatgctttt cttttgcatc tgcaaatatt cgttcaatgg tttgttttct ctttttgtaa
    21421 atttctctat tcaacgccgt atggcgttgg tgttcaagtt catccataag atcagcccaa
    21481 atatgtcgag tcactatttt ttgatgtttt gtactcaaag tacaattttc aagtaatgga
    21541 caactcacac agaattttgg atttgattta taggttcgat aaccatcacg agtgatagtt
    21601 gaaaataaca acggttgatt attgggacat aaatagcagt caaaataggc atcataaaca
    21661 aagtcttttt tacgaaacat tcctttcact ccatgaggtg cagtatatgg aagaatcggg
    21721 tagagtttca agtgtgacag aaaatgaaca aatttaggcg tcttatatcc tgcatcagca
    21781 accacacgct ttatatttgg aaaagctctt ttcgactgtt taaccagatc gattgcgact
    21841 tggctgtcat gcacatttcc tggtgtgatt aatgtagata agataaaacc gttatcttca
    21901 caggaagtat gtgcagaata agcaaattgt ttttctcgtt cacctttaac atagtaacca
    21961 ctttctggat ctgaatgact tattttttga agactcaact cactttctgt gtcccatgta
    22021 aaaggacgtt ttcctacagc aatacgttct ttatttattt catcttctaa ttcatcttga
    22081 taagcttttg cttgaccgta ggtcatctct ttcgtgaact tatgtttatt ggcatttgct
    22141 ttgatatgag tagaatcaat ataaaggttg tcttccgtaa cgaagccagc ttttacagct
    22201 tgttctagaa tatgagagaa aatatcttca aataaagttg tttctctaaa tcttcgaaca
    22261 tagtttttac caaaagtaga gaagtgtggg ataggatcct cgaatgaata acctaaaaac
    22321 caacggtaag caacatttgt ttcaacgtct ttaattgtct gtctcatcga acggatacca
    22381 aataaatatt ggataaatac taatttaatt aagaccactg gatcaatact gggacgacca
    22441 taggttgaat acgtagattc tacaatggga taaatgaaat caaactgaat cgcagcatca
    22501 atttttcgaa ctaagtgatc tttaggtaca agatcatcaa ttgttaacat agcaaattga
    22561 tttctacctt caatcgattg ttttctcatc attagaatca ctcccagaat agtttataca
    22621 attagtataa aacaaaaaac tgcaaacaac actccaaatt taggagtttg tctacagtct
    22681 gaatagagct gaaaagctct attttttttg ataaaaaaac tagctatttt attaaaaata
    22741 atttggttca aaatttattt ttttaacttg ataatttttt gccatcctt
//
LOCUS       DAZKLD010000034        22639 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.034, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000034 DAZKLD010000000
VERSION     DAZKLD010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22639)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22639)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22639
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.034"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            108..1604
                     /gene="glpK"
                     /locus_tag="ACG8KR_001074"
     CDS             108..1604
                     /gene="glpK"
                     /locus_tag="ACG8KR_001074"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HFQ0200462.1"
                     /translation="MTESTYIMAIDQGTTSSRAIIFDKKGRHIGSSQKEFTQYFPQES
                     WVEHDANEIWNSVQSVIAGAFIESGIKPNQIAGIGITNQRETTVIWEKDTGRPIYHAV
                     VWQSRQSAGIADNLKNEGYQEFFHEKTGLVIDAYFSATKIRWILDHVEGAQERAEKGE
                     LMFGTIDSWLVWKLTDGQAHVTDYSNASRTMLFNIHQLDWDQEILDLLNIPRVMLPKV
                     TSNSEVYGYTQGYHFYGSEAPISGMAGDQQAALFGQMAFEPGMVKNTYGTGSFIVMNT
                     GEKPQLSKNNLLTTIGYGINGKVYYALEGSIFVAGSSIQWLRDGLQMLQKASDSEDAA
                     KRSTSEDEVYVVPAFVGLGAPYWDQAARGAMFGLTRGTTKEDIIKATLQSIAYQVRDI
                     IDTMQDDTGIKIPVLKVDGGAANNEYLMQFQTDILNVPIQRAENLETTALGAAFLAGL
                     AVGYWKDTDEIREFYEAGKLFEVQMEEERREKLYNGWKKAVKATQAFE"
     gene            1618..3441
                     /gene="glpO"
                     /locus_tag="ACG8KR_001075"
     CDS             1618..3441
                     /gene="glpO"
                     /locus_tag="ACG8KR_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HFQ0200463.1"
                     /translation="MFSNKTRQDSIQKMQQEELDLLIIGGGITGAGVAVQSAASGIKT
                     GLIEMQDFAEGTSSRSTKLVHGGIRYLKTFDVEVVADTVGERAVVQSIAPHIPKPDPM
                     LLPIYEGEGATTFNMFSVKVAMDLYDKLANVTGTKYENYTLTPEEVLEREPFLKKEGL
                     KGAGVYLDFRNNDARLVIDNIKKAAEDGAYLVSKMKAVGFLYDGDQIVGVKARDLLTD
                     EVIEIKAKLVINTSGPWVDKVRNLNFTRPVSPKMRPTKGIHLVVDAKKLPVPQPTYFD
                     TGKQDGRMVFAIPRENKTYFGTTDTDYQGDFTDPKVTQEDVDYLLDVINHRYPEANIT
                     LNDIEASWAGLRPLLIGNSGSDYNGGDNGSISDKSFNKVVDTVSEYKENKVSRAEVED
                     VLNHLENSRDEKAPSTISRGSSLEREPDGLLTLSGGKITDYRKMAEGALRLIRQLLKE
                     EYGIETKEIDSKKYQISGGNFDPTKLEETVTELAKEGVAAGLEEEDATYIADFYGTNA
                     RRIFELAKEMAPYPGLSLAESARLRYGLEEEMVLAPGDYLIRRTNHLLFERDQLDEIK
                     QPVIDAIAGYFEWTEEEKAQQTKRLEALIAESDLRELKGEK"
     gene            3442..4158
                     /locus_tag="ACG8KR_001076"
     CDS             3442..4158
                     /locus_tag="ACG8KR_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HFQ0200464.1"
                     /translation="MNSDMTQIMGEFIGTLILVLLGDGVCAAVNLNKSKAQASGWIVI
                     AFGWGLAVTMAVYISGFMGPAHLNPAVSLAMAMTGAISWNLVVPFIIAQVLGAFAGAI
                     LVWLSYLPHWNATKDESAILGTFATGPAIRNYPANVITELIGTFVLVLGLLAFGQNEF
                     APGTNVFAVGGLILAIGLSLGGPTGYAINPARDFGPRLAHAVLPIANKGTSDWAYSWV
                     PIAGPMIGAIIAVGVYSLMV"
     gene            4464..6393
                     /locus_tag="ACG8KR_001077"
                     /pseudo
     CDS             4464..6393
                     /locus_tag="ACG8KR_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336853.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
     gene            6519..7073
                     /locus_tag="ACG8KR_001078"
     CDS             6519..7073
                     /locus_tag="ACG8KR_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HFQ0200465.1"
                     /translation="MKLSTDWRKEIQTIPNLLSIFRIFLLPIYLYFVLRQSFYIAGAV
                     IVVSGLSDYLDGVIARRYNQVTDLGKVLDPFADKLTQLFLILSMAWYRPWLWLLFGLF
                     LIKEGFMFVAGLIGLSKNIKLSGAKWYGKVATAVIYVGMILLLLFPELPTLWVRVIFA
                     VITYGLLQSFVLYAVEYRKMFQRK"
     gene            complement(7803..8117)
                     /locus_tag="ACG8KR_001079"
     CDS             complement(7803..8117)
                     /locus_tag="ACG8KR_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HFQ0200466.1"
                     /translation="MKVVFHIDELEKWSETGKNVKNLIKASPETTIVVSVNGIAITGY
                     LDSANAEFLDLQGVVFHACANAMRANHISESSLPEQVIVVPAGVLDLVELQSQGYAYI
                     KP"
     gene            complement(8131..8391)
                     /locus_tag="ACG8KR_001080"
     CDS             complement(8131..8391)
                     /locus_tag="ACG8KR_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HFQ0200467.1"
                     /translation="MEIDNKNILNRLKRTEGQIRGIQKMIEDEKECMDVITQLSAVRS
                     SIDRVMGMIVADNLKNCFEKPDSNPEEQAAKLEQAIKMIIKK"
     gene            8561..8875
                     /locus_tag="ACG8KR_001081"
     CDS             8561..8875
                     /locus_tag="ACG8KR_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFQ0200468.1"
                     /translation="MNLLFQSIKSITVPELKEKLLENPALLDVRTPAEYRGGHIKGAK
                     NVPLQSINRYDGDKEKTVYVICQSGMRSKQAAKELKKSGYDVVNVRGGMNQWFDRTVG
                     GK"
     gene            8877..10532
                     /locus_tag="ACG8KR_001082"
     CDS             8877..10532
                     /locus_tag="ACG8KR_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFQ0200469.1"
                     /translation="MKIVIVGGVAGGMSAATRLRRLMEDAEIVVFEKGPYVSFANCGL
                     PYYLSGEISERENLLVQTPESLSARFCLDVRPNHEVTAIFPENKTVEVVHEGQKHIEQ
                     YDALVLSPGAKPVVPSIPGITEADNVFSIRNVPDIDKVMHALEKQPKRAVIVGAGFIG
                     LEMAENLKRRGLEVMVIEQAPHILPTLDEEMAAFIEKELSHQGVEVITSHAVAGFEDH
                     GKRLRLDDGRTIPADLVILSIGVRPDNQLAVTAGIELGIRGGILVDERYQTNIPDIYA
                     VGDAIVVKQQITGKDALISLASPANRQGRQVADTISGISRRNQGGIGTAIIRTFGMTA
                     ASTGLSERTAKENELSFEVIHVSGKDHASYYPEATDILLKLIFHPETGEIYGAQGVGA
                     KGVDKRIDILATAIKGHLTIFDLPELELTYAPPFGSAKDPVNMLGYAAMNIAEGLSET
                     VQWHELPTELAKGKILLDVRTAEELEKGKFKEAKHIPLNELRDRLNELDSQQEYIVSC
                     HSGLRSYLAERILKQSGYHVKNLDGAFSLYQTVRPEELIYPNK"
     gene            10615..12750
                     /locus_tag="ACG8KR_001083"
     CDS             10615..12750
                     /locus_tag="ACG8KR_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HFQ0200470.1"
                     /translation="MVNTQKQLEEAYLKNVYQKLIDKKNELRESMDTAKIEGNQSLKE
                     MAGELRLNFDSYLDNLDTFSMIEMKNREIDQMNIKQKNAENQLAKIERLLKSPYFGKV
                     VVDFLDNEPKESFYIGTANFTDDADENLVYDWRSPIAELFYNNMIGPSSYTVRQNKID
                     TVIKERRQFLLERDRLVKFFDTSVAIQDDVLLEVLGQDETNEMKAITATIQSEQNTII
                     RDVKSQNILVNGVAGSGKTSAIMQRIAYLLYLYRDTMTSDDLLILSPNHRFIDYISNV
                     LPSLGERNPLNLTIIQLVSQLSAEEIEGEEAYFKRITGENVSEQTERLRSKKFIDNLK
                     QSDPLFLDHPNFIRGLTKNGKTVLSKKTIEKIYEKVPAHPKLIDRLQATKKALMSEWK
                     NHLLKQAKSPAVQNQVLSLTEDRQLELFGKLISDDSEQSIVAYARKLLQKKYRKITRQ
                     IEEMAWVDEHQLFERIYEKRYGSAYAWQPTRTVDEAVIILAIRHLLVEKVNVPAFRYL
                     LIDEVQDYTLAQLGLLIELFPKTHFTLVGDENQAIFNSSTTFADIMRCFDDYHLPIHR
                     YDLRNSYRSSGAITELFKTYAVDQEKIDIVSIRPQGKEPEYRSFRSSEELLKILAEIL
                     VSLKGEKAAVITQTEEETQTLQESIKKNSYGLENCQIIPLSLAKGLEFDHVILYPFEN
                     DGDEQRRRRQMYTAISRGMKSIVVLERAT"
     gene            12925..13434
                     /locus_tag="ACG8KR_001084"
     CDS             12925..13434
                     /locus_tag="ACG8KR_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0200471.1"
                     /translation="MKKVTREEVASILVKDKYFSKGNYWLKIWQTLVALLGWMIVVLP
                     FIWVFFPLTRPERAKDFSLYAFLSEKKMLYFLIGFFVLIFFSFLITSFVLTRWNNRNL
                     YKKVNKSFEYEDEKLKKRQELLEEVYTERFGTKEERETVRFYSVSEEKNLDTTFIADL
                     YKENGVELK"
     gene            13431..14735
                     /locus_tag="ACG8KR_001085"
     CDS             13431..14735
                     /locus_tag="ACG8KR_001085"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFQ0200472.1"
                     /translation="MISITHTGNLFLDTCLSIMYFFLVSYPILGGFVWFIGVWCYVFL
                     YKHKQKEWVDVPLSVEPFITIMVPAHNEEIVIEYTIEYLMTKLNYHNYEVLVTDDGST
                     DQTPEILARLMKKYANLRVVRIEKNKGKAHAFNIGLAFAKGKLILSNDADTVPEPDAL
                     IRYVNYFIRPGARHIAAVTANMDVQNRTKLIAKSQTVEFSSIVGIIKRTQSAVFGGLY
                     AYSGANTMYRKEALIDVGGFRQDRATEDISIAWDHQLNDWLSVFAPEIIFFMEVPVTL
                     KMLYRHRKRWAKGGTEVWLTNFKKVMLHPFKHIGRTIIFIDQTLSIVWSIFFCISVVL
                     FAGLIGHYVYQGNYEQIYITFTFSFVFICFEMVAGFFQLLASLIVDDRRRKLKYLLFA
                     PLYMLLFWIVNAITIVTTFIPAVKTILGYGSGTWKSPERTKK"
     gene            14843..15700
                     /locus_tag="ACG8KR_001086"
     CDS             14843..15700
                     /locus_tag="ACG8KR_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HFQ0200473.1"
                     /translation="MKKRMKALIWPIAVSSIFLLGQTAFAETSEPAQTEPSTTISSSG
                     AENGASETEMTSNVPTDNSGETISSSEDTSINSSTSSDQIDHLQDLENKRKELEEALE
                     AAKNKLKEDQAAIDQDLADLKNKVGNDRAQLEKKLAEAKAKAEAVQTDLDGKIQQAKE
                     FNQQLNQVAQTASGKLSQTQADITAASQAIHGKISEAQTVANDFEAKKADLAASFQRI
                     KDILHSAGEKSVTSGSTTETVQQTTEPSKSTSQTLPKTNEKTASVSYSLAGAGVLVAA
                     AYGIIRKKK"
     gene            complement(15812..16132)
                     /locus_tag="ACG8KR_001087"
     CDS             complement(15812..16132)
                     /locus_tag="ACG8KR_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HFQ0200474.1"
                     /translation="MITTTTNSVEGKAVVAYKGIVFGEVITGIHAFKDLEAGLRNIFG
                     GRSKSYENELMGAREEALAEMAERAKALGADAIIGVKMDYEVLGSDNGMLMVTCSGTA
                     VSTR"
     gene            16286..16681
                     /gene="dhaM"
                     /locus_tag="ACG8KR_001088"
     CDS             16286..16681
                     /gene="dhaM"
                     /locus_tag="ACG8KR_001088"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase phosphoryl donor subunit
                     DhaM"
                     /protein_id="HFQ0200475.1"
                     /translation="MKKSILLISHSQKVTDGIKEMIEQMQKSEEVQIFSLGGINGEIG
                     SDPMKIVEAVNESSDAASYFVFADIGSAVLNSELAKDMLEEEQQQRYHLVDAPLVEGA
                     FAAAITAGITDDTQQILTEAQNAGKKGWE"
     gene            16656..17666
                     /gene="dhaK"
                     /locus_tag="ACG8KR_001089"
     CDS             16656..17666
                     /gene="dhaK"
                     /locus_tag="ACG8KR_001089"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HFQ0200476.1"
                     /translation="MPERKDGNKLKKIMNDPSNIVEEMLEGLVKSYPELVHRVESSRV
                     VAKNQKAEQVGLVSGGGSGHEPSHAGFVGEGMLSAAVLGDVFTSPTPDQIQTAIKEAD
                     SGKGVLLIVKNYTGDALNFDMAKELAAMDDIEVESVIVDDDIAVENSTYTAGKRGVAG
                     TVLVHKIVGDAARNGASLAELKELGEKVVQATKTIGLALRAATVPEVGKPGFELGEDE
                     IEYGVGIHGEPGYRREKMQPSKVLAKELVTKILDDYSNLPKEAGVLVNGMGGTPLMEQ
                     FVFMNDVLALLEERGVNVVFRKVGNFMTSLDMQGLSLTLIDLTETQWKDSLESNVQTI
                     SW"
     gene            17694..18299
                     /gene="dhaL"
                     /locus_tag="ACG8KR_001090"
     CDS             17694..18299
                     /gene="dhaL"
                     /locus_tag="ACG8KR_001090"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaL"
                     /protein_id="HFQ0200477.1"
                     /translation="MKLTVQEIQEWLTQFAEAINQNKQYLSDLDTPIGDGDHGNNMGR
                     GVSAYEEAFQADHPETISDTFKVFSMAMISKVGGASGPLYGSAFMNMMKATKEVDSID
                     SQEKLGEVIEQGTAGIQSRGKAEADDKTMLDVWLPVTEALKAGNLTKEIIDQAKEHTK
                     VLVAKKGRASYLGERAIGHIDPGAASSAILFSTLLDVINQP"
     gene            18446..19111
                     /locus_tag="ACG8KR_001091"
     CDS             18446..19111
                     /locus_tag="ACG8KR_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate 1-dehydrogenase family
                     protein"
                     /protein_id="HFQ0200478.1"
                     /translation="MELGKSDYQLFDRPIYAFKQLKESHPTDKIEQIKKEYKEHWQKW
                     KEIQLQTAALLPDMYGMSKPKIESWTNGWNLRSHFWSAYRSESRQDENACLAVLLNQK
                     QYQIYLMYQHYKSEERYGSIAAYNQLLAILKEWSKTVDIKDYYIWPQPEHELDDHLAL
                     SDYLSDTKKQEELKKAMRDRTFQMGKLFFYPQEIEHVEQITAETLQELAPLYQKLGAE
                     KFQ"
     gene            19394..20053
                     /locus_tag="ACG8KR_001092"
     CDS             19394..20053
                     /locus_tag="ACG8KR_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFQ0200479.1"
                     /translation="MSHDHLCVSFVPLFNHLVQQDQEKVHALVKHHYVEKGEQILAPN
                     GEPQLVIVASGSMKVYQLSASGKEQLLRVIEPGGYEGESQLLGVPNESLFGEALEKTE
                     ICILRQKDFSELLLHYPALSLKLLAINVEKLTKVQQQTTFLMMEKVEERVAVYLLDLA
                     KATGKDHFFLPMKMKELAAFIGTTPETLSRKFKLLEENGYVKRKNREVWIIDKEQLED
                     L"
     gene            complement(20130..21740)
                     /locus_tag="ACG8KR_001093"
     CDS             complement(20130..21740)
                     /locus_tag="ACG8KR_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0200480.1"
                     /translation="MTDKFSRWSTLFSQNLSRDWKKILIWLVGVTAFSGGFVPAFEEL
                     TSGQGLAGLYETLQNPAMIAMVGPTPASNAAEYTLGAMYANEMLLFCSLFAAVISVLH
                     VVSQTRKKEELGLIELIRSFRVGRQANSLAVIAESILINLLLAISISCTMIFFQADSI
                     TAEGAWLFGLSVGSMGILGSAVALFAAQLMPSSASSNGLSLAFLGGLYLLRAITDVAD
                     SSLSMWNPLGWSYLTYPFTTNNWIPLLYLFLFSLILIISSFVLEEHRDLNTGYIPERE
                     GKPYAKRSLLSLRGLLFYLNKGMTISWLITFGLMGAAYGSIYGNMQQFLESNDLMKQM
                     FIQSGVSIEKNFTSTIMVVLICLAAILPIASINRLFTEETHGRMNQLFATKISRNQLY
                     WTNMLLAILFAILAILISSGALGLSALASMKDAKDMDLLMFLKAGFNFLPAVLVFIGI
                     SGFLFGWLPRIGKLAYIYLAYSFALNYFGGILDLPEWFSKTAVPSWVPRMPNDSFDLS
                     VFLTLSCISLALFVLGSIGYRRRDLLEG"
     gene            complement(21753..22634)
                     /locus_tag="ACG8KR_001094"
     CDS             complement(21753..22634)
                     /locus_tag="ACG8KR_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFQ0200481.1"
                     /translation="MAEIVKVTHLKKSFGRFEALKDVSFTVHSGEVVGFIGPNGSGKS
                     TTIRTLLGIIKKDGGTATIFGQDVWEDSLSIHERLSYVPGDISLWGNLTGGAIIDLFM
                     KLHGKGSAQKKDELIQRFSLDPKKKAKNYSKGNRQKVGLIAALAVDSDLYLFDEPTSG
                     LDPLMETIFQEEVEKLKEQGKSILLSSHILSEVERLADKVVIIREGTLDEEGTLDELR
                     HLTRSTITIETEAENSHLAQLNGVHDFVQKGHLAQFAADNEAFDVILAEASKLGVKKF
                     ESIPPTLEDLFMRHYEG"
BASE COUNT         7549 a         4054 c         4859 g         6177 t
ORIGIN      
        1 gtcaaaaaaa gaaaaaaaga ataaaatttt gacagtttct ttttttaaaa cagtagtatg
       61 ataagagtag ttaaagaaag cgtttgcata taatggaggg aatcacaatg acagaatcaa
      121 cgtatatcat ggcgattgac caaggtacaa ctagctcacg agcaattatc tttgacaaga
      181 aaggaagaca catcggcagt tctcaaaaag agttcacgca atatttcccg caagaaagtt
      241 gggtggaaca tgatgcaaat gaaatctgga attctgtaca gtcagttatt gccggagcgt
      301 tcatcgaatc agggatcaaa ccaaatcaga ttgctgggat tggcataacg aaccagcgtg
      361 aaacaacagt aatatgggaa aaagatacag gacgtccaat ctatcatgca gttgtttggc
      421 aatccagaca gtcagctgga attgcagata acttaaaaaa tgaaggctat caagaatttt
      481 tccacgaaaa aacagggtta gtgatcgatg cctatttctc agcaacgaag atacgctgga
      541 ttcttgatca tgtagaaggc gcgcaagaac gagctgaaaa aggcgaattg atgtttggaa
      601 cgatcgattc ttggttagta tggaaactga ccgatggaca agcacatgtt actgattact
      661 cgaatgccag tcggacgatg ttgttcaata tccatcagct agattgggat caggagattt
      721 tagatttact aaatattcct cgagtgatgt taccaaaagt cacttctaac tcagaagttt
      781 atggttatac gcaaggttat catttttatg gaagcgaagc accaatttca gggatggcag
      841 gagaccagca agccgcatta ttcggacaaa tggcattcga accaggcatg gtcaagaata
      901 cttatggtac aggttcattt attgtcatga ataccggtga aaaaccgcaa ttatccaaaa
      961 ataatttatt gacgacaatt ggttacggca taaatggaaa ggtttactat gctttagaag
     1021 gaagtatttt tgtggctggt tcttcgattc aatggctgag agatggactt caaatgttgc
     1081 aaaaagctag cgattcagaa gacgcagcga aacgctctac aagcgaagat gaggtttatg
     1141 ttgttcctgc attcgttggg ctaggtgctc cttattggga tcaggcagca cgaggggcta
     1201 tgtttggctt gacacgtggt acgacaaaag aagatatcat taaagctact ttacagtcga
     1261 ttgcttatca agtgagagat atcatcgata cgatgcaaga tgacactgga atcaagattc
     1321 ctgtattgaa agtagatggc ggtgcagcaa acaatgaata tctgatgcaa ttccaaacag
     1381 atatcttgaa tgtaccgatc caacgagcag aaaacttgga aacaacggcg ctaggggcag
     1441 catttttagc aggattagct gtcggttatt ggaaagatac agacgagatc cgcgaatttt
     1501 atgaagctgg caagctattt gaagttcaaa tggaggaaga gcgccgagaa aaactttaca
     1561 acggttggaa aaaagcagta aaagctaccc aagcatttga ataaggagga aatccatatg
     1621 ttttcaaaca agacaagaca agatagcatt caaaaaatgc agcaagaaga attggatctg
     1681 ttgatcatcg gtggcggaat cactggtgcc ggtgtggcag tccagtcagc agcatcagga
     1741 atcaaaacag gattgatcga aatgcaggat tttgcagaag ggacttcctc tcgctcgacc
     1801 aaacttgttc atggcggtat tcgttatctg aagacatttg atgtggaagt agtagctgac
     1861 acagttggtg aacgtgcagt cgtacaaagt attgccccac acatcccaaa accagatcca
     1921 atgcttttgc caatctatga gggcgaagga gcaacaacct tcaatatgtt ctcggtcaaa
     1981 gtagcaatgg acctttatga caaactggca aatgtgacag gaaccaaata tgagaattat
     2041 acgctgacac cagaagaagt attggaaaga gaaccgtttt tgaaaaaaga agggctaaaa
     2101 ggcgcgggtg tctatctaga tttccgcaac aatgatgccc gtttagtgat tgataatatc
     2161 aaaaaggctg ccgaagatgg ggcttatcta gtaagtaaaa tgaaagcggt tggcttttta
     2221 tatgatggcg atcaaatcgt tggcgtcaaa gctcgtgacc tgctgacaga tgaagtgatc
     2281 gaaatcaaag caaaattagt gatcaatacg agtggtcctt gggtagataa agtacgaaac
     2341 ttgaatttta cgcgtccagt ctctcctaaa atgcgtccaa ctaaagggat ccatttagtt
     2401 gtagatgcga aaaaactgcc tgtaccgcaa cccacatact tcgatacagg aaagcaagat
     2461 gggcggatgg tttttgctat tccaagagaa aacaagactt actttggtac gacagatacg
     2521 gattaccaag gagattttac ggatccgaaa gtcacacaag aagatgtaga ttatctattg
     2581 gatgtgatca atcatcgcta tccagaagca aatatcacat tgaatgatat cgaagcaagc
     2641 tgggcaggac ttcgtccact gttgattggt aattctggtt ctgattacaa tggtggagat
     2701 aatggatcga tttcagacaa gagcttcaat aaagttgtcg atacagtaag tgaatataag
     2761 gaaaacaaag tttctcgtgc tgaagtagaa gacgtgttga accatttgga aaacagccgt
     2821 gatgaaaaag caccttctac gatttccaga ggtagttctt tagaaagaga accagatggt
     2881 ttgctgactt tatctggtgg gaaaatcacc gattaccgta agatggcaga aggagctttg
     2941 cgattgatac gccaattatt aaaagaagaa tacggaatag agacgaaaga aatcgactct
     3001 aaaaaatatc agatttcagg tggaaacttc gatccaacta aattagaaga aacagtgaca
     3061 gaattagcaa aagaaggagt agcagccggt ttagaggaag aagatgctac ttatatcgct
     3121 gatttttacg ggactaatgc ccgacgtatc tttgaattag caaaagaaat ggcaccttat
     3181 cctggtttga gtctcgctga gtcagctcgg ttacgttatg gtttagaaga agaaatggtt
     3241 ttggccccag gcgattatct cattcgtcgt acaaatcatc tgttgtttga acgagatcag
     3301 cttgatgaga tcaagcaacc tgtgatcgat gcaattgctg gatattttga atggacagaa
     3361 gaggagaagg cgcaacagac taaacgttta gaagcattga ttgcagaatc agatctgcgg
     3421 gaactaaagg gggagaaata aatgaacagt gatatgactc aaatcatggg ggagtttatt
     3481 gggacactga tcctagtttt gcttggagat ggtgtgtgtg cggctgtgaa tttaaacaaa
     3541 agtaaagcac aggcttctgg ctggattgtc atcgcatttg gttggggact cgctgtaaca
     3601 atggctgtct atatttctgg attcatgggc ccagctcatt tgaatcctgc agtctctcta
     3661 gctatggcga tgacaggggc aattagttgg aatcttgtcg ttcctttcat cattgcgcaa
     3721 gttttaggag cttttgcagg ggccattctt gtttggcttt cttatttgcc acattggaat
     3781 gcaacgaaag atgaaagcgc gattttagga acgtttgcaa caggtcctgc tatacggaat
     3841 tatccagcaa atgtgatcac agaattaatt ggtacatttg tattagttct tgggttgcta
     3901 gcttttggac aaaacgaatt tgcacctgga acgaacgtat tcgctgtagg tggattgatc
     3961 ttagccatcg gtctttcatt aggagggcca acaggatatg caatcaaccc tgctcgggac
     4021 tttggtcctc gattagcaca tgcggtattg ccaatagcta ataaaggaac ttcagattgg
     4081 gcatattcat gggtaccaat cgcaggtcca atgatcggag caattatcgc ggtaggtgtc
     4141 tattcattaa tggtgtaaat ctataaaaca tactaaagtt tgacaaccaa aatgtgtctt
     4201 gctatagtta agtggaaaat aaataagacc tagaagaatg gggtatgttt tgtgagtgta
     4261 gctggtatct aatcagtaag tcgaatagcc aataaacaaa gtttatttgg tgtgcttatg
     4321 aaacggtacc tgtcaggaaa acaatatctt tgtattctaa taaggatagg acatgacttt
     4381 tttaagctgt gttctatcct tttatttatc tctaggccct tatttatttt gggacaggta
     4441 tctcttcaag aaaaggagag aatatggaca acgaaatgat tagtcaatta caatttgatc
     4501 ggatcaaaaa ggaaatccaa gcaagagcaa ttggaaacta tagcaaaaaa cgaatcagcg
     4561 atatgacggt ctcgacaaac ctacaaactg tcttggatcg tcaagaagaa acaagagaag
     4621 cacgcttgat cttagaaagc agtcagcatg ttccatttat ggggttgccg aggatcgatg
     4681 cactgacaga acaagtcaaa aaagggttga ttctgcagcc aaccgatttg atcgaatatg
     4741 cggattttct tagaagcagt cggatgatca caaagttttt tgataagaat caataccaga
     4801 cacccttgtt gtttgcttat agcaaacatc ttcctgattt gataaatgta gaagagttga
     4861 ttgaccagaa aataaaaaat aataaagtca gtgacgacgc gtcacgtaat ttgcgtaaag
     4921 tacgcaaaca attacaacta atcgaaaaag aaatccagtc aaaattactg aaattcttgc
     4981 gtcatccgaa aaacaaagaa atgatccaag aagcaatgat tgttcaaaaa ggcgaatact
     5041 atacgattcc tatcaaagcc agctataaaa ataaaataga tggaacgatt atcgatgaat
     5101 caaataaaag aacgacggtt tttattgaac caactgtgat ttctaaattg aatgaacact
     5161 atcagttgtt aaaagctgaa gaaatcagtg aagaatatca agtcctggcg gcattgactg
     5221 gtgctatcgc agaaaacgaa gaagtgatcg atttattgat cgaaacgatc acggtgttag
     5281 atatcatctt tgctcgggca aaatttagcc gagaaatcaa tggaagcaca cctagaatca
     5341 ataaatcgga acatatcatt atcaagcaag gcagacatcc atttttacct gagcatgcgg
     5401 taccattgga tgttgaaatc ggtaaagact atcgaggctt gattatcacc ggagcaaatg
     5461 ccggaggcaa aacagtcgtg ttgaagacag tcggtttatt gactttgatg gcgatgttcg
     5521 gtatgcaagt tccggcaaaa gaagggacag agcttgctgt ctttgatgag attttcgtcg
     5581 atgtcggtga ccaccaaaat ttagaaaatg cgttgagtac attttctggt catatgcaaa
     5641 acatcgctgc tattttgaaa aagataaaaa ggaatacgtt ggtactttta gatgagattg
     5701 gcagtggaac agagccaaat gaaggggctg cactagcgat cgcaatcatg gaatctatgt
     5761 atgagcaagg ggcattgatc attgccacga cccattatgg agaaatcaag aaattcgccg
     5821 gagatcatga agattttgtg ccggctgcta tggctttcga tcgggaagca cttcgtccta
     5881 aatatcagtt gcgggttgga agaaacagga gaaagccaag ctttgtggat tgctcataaa
     5941 atggatatgt cgatgaaatt gatccatcaa gcagagcgtt acttggcgga aaaagcctat
     6001 cggacacaga aaaaagaatt tcttcctaag acagcagtta caaataggaa ggagaataaa
     6061 aaagaacgtc agctctttgc aaaaggtgat cggatatttg tcaatgaata ccaaaaagaa
     6121 gcgttagttt atgaagatat aggtgaagat acgattgatg tatatttaga caaaaaaata
     6181 atccacgtgc ctcgtcagcg tgttcgtctg gtgcgttcag cagaagatct gtacccgaca
     6241 ggttacgatt tagacagtct gtttatcgat tataaaacac gtaaacggca gcgtgaccta
     6301 gagcgaggat caaaaaaagc tcataaagta ctagtaaaag aaatgcgcaa acgacaggaa
     6361 gaaagaagag taaacgatga aaacagcaag tgattatctc aaattagctc ataatatcag
     6421 tgtctgtaaa gaaaaataaa aacagtgaaa tcattggttt tattccccgt gtaagctaaa
     6481 ataaaaagaa aagagtgctg aggaggtagg gcgataaaat gaaattaagc acagactggc
     6541 gtaaagaaat acaaacgatt ccgaatcttt tatcgatatt ccgtattttt ttgctgccga
     6601 tttatcttta ttttgtgctg agacagtcat tttatatagc aggagcggtc attgttgtct
     6661 caggtttgtc tgattatcta gacggggtca ttgctcggag atataatcag gtaacagact
     6721 tagggaaggt gcttgatccg tttgcagata aattgacaca actttttctg attttgtcta
     6781 tggcatggta tcgaccgtgg ctttggctgt tgtttggtct attcttgata aaagaaggct
     6841 ttatgtttgt agctggatta atcgggctat ctaaaaatat caaattaagt ggagcgaagt
     6901 ggtatggcaa agtagcgact gctgtcatct atgttggcat gatccttttg ctgcttttcc
     6961 cagaattacc aacgctgtgg gtgagagtca tttttgccgt catcacttac ggtcttttac
     7021 aatcttttgt cttgtatgct gtcgagtacc gaaagatgtt tcaaagaaaa taaaaaaggg
     7081 gtgtgaccta tgtcgcattc ctttttttga taaatagact cccagtagct acttctcgat
     7141 aataagccaa ccgaaccaaa aacaggagaa gttgtttttg aaattatttc ttgtgattcg
     7201 gagttaaatg gtaggctcac aacctcttgg taaacggtgt tcaatcagct gaccttcggt
     7261 attagctcaa aaaacggcaa aatatgagca gctatttttc cgtttgtttc tctaatactc
     7321 aggtcaaacc gctgatttta caacctcttc cttaaacggt attcaaaagc cagtttctcg
     7381 gtattaagcc aaatttatgc aaaaatgtag aaatccattt tcacaaaatt tttcttaata
     7441 ctcgaaacta agcgactttt tcacaatctc ttccttaaac ggtgttcaat cagctgacct
     7501 tcggtattag ctcaaaaaac ggcaaaatat gaggagctat tttcccgttt ttttctctaa
     7561 tacttaggtc aaaccgctga tttcacaacc tcttccttaa acggtgttca aaagcttgca
     7621 tctgcggtaa taagcccaac caaacgaaaa atatgagaag ctatttttgt ttggctgttc
     7681 ttattacttg atgcaggaca gcgttttcac aacctttgat tcacggtttt ccagaagtag
     7741 cttcttcgaa aaataagccg aaatttcttg aagctgatca cttctgtcgc aacctctact
     7801 gactaaggct tgatataagc ataaccttgg gattgcagtt caactaaatc aaggacacct
     7861 gctggtacaa cgattacttg ttcaggtaaa gaactttctg aaatatgatt agcacgcata
     7921 gcattggcgc aagcgtgaaa aacgactcct tgcaaatcta agaattctgc attggcagag
     7981 tctagataac cggtaatcgc gatgccattt acagatacga caatcgtcgt ttctggagaa
     8041 gcttttatca ggttttttac gtttttccct gtttctgacc atttttctaa ttcatcaata
     8101 tggaaaacga ctttcattga aatcattcct ttacttttta ataatcatct tgatcgcttg
     8161 ttctagttta gccgcttgtt cttctggatt actatcaggt ttctcaaagc aatttttcag
     8221 attatctgca acgatcatgc ccattacgcg atcaatacta gaacgaacag cgcttaattg
     8281 agtgatgaca tccatacatt ctttttcgtc ttcgatcatt ttttggatac cacgaatttg
     8341 tccctctgta cgtttcaaac ggttcaggat atttttatta tcaatctcca taacacattc
     8401 tcctctattt ttataattag ataatacccc ttatcggtat tttatgcaaa tataaaaagt
     8461 agtgaaaaaa atgaagagtc attctggttg acatattaat aaagctcctg tttaatatac
     8521 cccatgaggt atttataaaa atgtaaatga ggtgcagaaa atgaatttgc tatttcaatc
     8581 aataaaatcg ataacagtcc cagaattaaa ggaaaagctt ttggaaaatc cagcgttact
     8641 tgatgttaga acacctgcag aataccgggg cgggcatata aaaggagcta aaaatgttcc
     8701 attacaaagt ataaacagat atgatggaga caaagaaaag accgtctatg tgatttgtca
     8761 atcagggatg aggagtaaac aagcagctaa agaattaaag aaatctggtt atgatgtagt
     8821 gaatgtacgt ggtggcatga atcaatggtt tgatcgaaca gtaggaggga aataagatga
     8881 aaatcgttat tgtcggaggt gtagcaggtg gaatgtctgc tgctacacgg cttcgccgat
     8941 taatggaaga tgcagaaatc gttgtttttg aaaaaggacc gtatgtttct tttgcaaatt
     9001 gcggcttgcc ttattatctt tcgggagaaa tcagtgaacg ggaaaatctt cttgtccaaa
     9061 cgccagaatc attatccgct cgtttttgtt tagatgtgcg tccaaatcat gaagtgacag
     9121 ccatctttcc cgaaaacaaa acggtagaag tcgtacatga gggtcaaaaa cacattgaac
     9181 agtacgatgc attggtttta tctcctggtg caaaaccagt tgttccatcg attccaggga
     9241 taacagaagc cgacaatgtt ttttctatta gaaatgtacc agatatcgat aaagtgatgc
     9301 atgcattaga aaaacagcca aagcgtgccg tgatcgttgg tgcaggattc atcggattgg
     9361 aaatggcaga aaacctaaaa agaagaggtt tagaagtcat ggtgatcgaa caagcaccac
     9421 atattcttcc aacactggat gaagaaatgg cagcttttat agaaaaagaa ttgtctcatc
     9481 aaggagtaga agtgattact tctcatgctg tcgctggatt tgaagaccac gggaaacgat
     9541 tgcgactgga tgatgggcgt accatccctg ctgatttagt tattttatcc attggtgttc
     9601 gtcctgataa ccagctagca gtgaccgctg gaatcgaatt aggcatacgc gggggtatcc
     9661 tagtagacga acgttatcaa acgaatattc ctgatattta tgcggtgggg gatgctatcg
     9721 ttgtaaaaca gcaaatcact ggaaaagatg cacttatttc tcttgcttca ccagccaatc
     9781 gtcaaggtag acaagttgcg gacacgattt ccggaatttc tcgaagaaat caaggcggta
     9841 ttggaacagc aattatacga acgtttggaa tgactgccgc atccaccggt ttaagtgaaa
     9901 gaacagccaa agaaaacgaa ctgtcttttg aagtcattca tgtatcagga aaagatcatg
     9961 caagctatta tccagaagca acagatattt tactgaagtt gatcttccat ccagagactg
    10021 gcgagattta tggtgcacaa ggtgttgggg caaaaggtgt ggataaacgg atcgatattt
    10081 tagcaacagc aatcaaaggg catttgacga tcttcgattt accggaatta gaattgacgt
    10141 atgcaccgcc atttggctca gccaaagatc cagtaaacat gctaggatat gcagcaatga
    10201 acattgcaga agggcttagt gaaaccgtac aatggcatga attgccgaca gaactagcaa
    10261 aaggaaaaat tttattagat gtgcgaacag cagaagaatt ggaaaagggc aaattcaagg
    10321 aagccaaaca tatccctttg aatgaacttc gggaccgatt aaatgaatta gacagccaac
    10381 aagaatatat cgtcagctgt catagcggat tacgtagcta tctagcggaa agaatcttga
    10441 agcagtctgg ctaccacgta aaaaaccttg atggtgcatt ttctttatat caaactgtcc
    10501 gacctgaaga actgatatat cctaacaaat gatactcgtt aaaaagcttg cattcgactc
    10561 ttaaaagagg taaactaatc ggttccacca ctttaaaaaa agaggagaat gtgtatggtc
    10621 aacacacaaa aacagttaga agaagcatat ctgaagaacg tgtatcaaaa gctaattgat
    10681 aaaaaaaacg aactgcggga atcgatggat acggcaaaaa tagaaggaaa ccagtcgttg
    10741 aaagaaatgg ctggcgagct tcgattgaat tttgacagtt acttagataa cctggatact
    10801 ttttcgatga ttgaaatgaa aaatagagaa atcgatcaga tgaatatcaa acagaaaaac
    10861 gcagaaaatc agctagcaaa gatcgaacgt ttattgaaaa gtccctactt tggaaaagtc
    10921 gttgtagact ttctggataa tgagccaaaa gaatcctttt atatcggtac agcaaatttc
    10981 acagatgatg cagatgaaaa tcttgtttat gattggcgtt caccaattgc cgaacttttt
    11041 tacaataaca tgattggtcc atcttcttat acggttcgtc aaaataaaat cgatacggtt
    11101 attaaagaaa gacgacaatt tcttttggaa agagatcgtt tggtcaagtt ttttgatacg
    11161 tctgtagcta ttcaagatga cgtattatta gaagtcttag gccaagatga gaccaatgaa
    11221 atgaaggcta tcactgctac gatccaatcg gaacaaaaca caatcattcg ggatgtgaaa
    11281 agtcaaaata tcttggtaaa tggcgtagca ggaagtggta aaacctctgc tatcatgcag
    11341 cgtattgcgt atctgctcta tttatatcga gatacgatga catccgatga cttgctgatt
    11401 ctatcaccta atcatcggtt tatcgactat atttcgaatg tcctaccatc attaggtgaa
    11461 agaaatccac tgaatctaac catcatacag ctggtttcac agctttcagc agaggaaata
    11521 gaaggtgaag aggcttattt caaacgtatc acgggagaaa atgtatcgga gcagacagaa
    11581 cgtttgcgta gcaaaaagtt tatcgataat ctaaaacaat cagatccttt gtttttagat
    11641 catccgaatt ttatccgtgg acttactaaa aatggaaaaa cagtcctttc taaaaaaacg
    11701 atcgaaaaaa tatatgagaa agttcctgct catcctaaat tgatcgatag gcttcaagca
    11761 acgaaaaagg cactgatgag cgaatggaag aatcatcttt tgaaacaagc aaaaagtcct
    11821 gctgtgcaga atcaggtact ctcgttgaca gaagataggc agttggaatt attcggtaaa
    11881 ttgatttctg atgattcgga acaaagtatt gtagcttatg caaggaaact tttacaaaaa
    11941 aaatatcgaa aaataaccag acaaatcgaa gaaatggcat gggtggatga gcatcagttg
    12001 tttgaaagaa tttatgaaaa acgatatggc tcagcttatg cttggcaacc cacacgtact
    12061 gttgatgaag cagtcattat attagctatt cgtcatttac ttgttgaaaa ggtaaatgta
    12121 ccagcatttc gctatctatt gattgatgaa gtacaagatt ataccctggc acaattaggt
    12181 ctattgatcg aattatttcc aaaaactcac tttactttag taggtgacga gaatcaggca
    12241 atctttaatt cttccactac ttttgctgat attatgcgtt gtttcgacga ctaccatctg
    12301 ccgatccatc ggtatgatct tagaaatagt taccgttcta gcggagcaat cacagagctt
    12361 ttcaaaacct atgctgtgga tcaagagaag atcgacatcg tatcgatccg accgcaagga
    12421 aaagaacccg aataccgctc ttttcgatcc tctgaagaat tgttgaaaat actagctgag
    12481 attttggttt ctctaaaagg agaaaaagcg gcagttatca cccaaacaga agaggaaacc
    12541 caaacgctac aagaatcgat taaaaaaaat tcatatggat tagaaaactg ccaaatcatt
    12601 cctttgagtc tagcaaaagg actggaattt gatcatgtaa tcttatatcc atttgaaaac
    12661 gatggagatg agcaaagaag acgcagacaa atgtacacgg cgatatctag aggaatgaaa
    12721 agcattgtcg tattggaaag agcaacataa ttaagagata aaaaataaaa agaacaactg
    12781 ataaaaagaa caaaatattt ttcatttgct gatgagaaat gttttgttct ttttcttagg
    12841 aaaaatttag gaacacttta tgtgaaaaga aatgaaatgt atggtaaact cctacgtcgt
    12901 aacaaaaaaa cgtgaggtga ggatatgaaa aaagtaacaa gagaagaagt tgcatcaatt
    12961 ttggtgaaag ataagtattt ttcaaaaggt aattactggt taaagatttg gcaaacgtta
    13021 gtggctcttt taggatggat gatcgttgtt cttcctttta tatgggtctt tttcccatta
    13081 acgagaccag aaagagccaa ggatttttcc ttgtacgctt ttttatcaga aaagaaaatg
    13141 ctgtatttct taatcggctt ttttgtattg atttttttca gtttcctgat tacctcattt
    13201 gtattgacta ggtggaacaa tcgaaatcta tacaaaaaag taaataaatc ttttgaatat
    13261 gaagatgaaa aattgaaaaa aagacaagaa ttacttgaag aagtgtatac ggaacgtttt
    13321 ggaacaaaag aagagagaga aacggtccgt ttttattctg tctccgaaga gaaaaatcta
    13381 gatacgacat tcattgcaga tttgtataaa gaaaatgggg tggaactgaa atgatctcaa
    13441 tcacccatac aggtaattta ttcttagata cttgtttgtc tattatgtat ttttttctcg
    13501 tttcttatcc gattttaggc ggatttgtat ggtttattgg tgtctggtgt tatgtgtttt
    13561 tatacaaaca taagcaaaaa gaatgggtag atgttccctt gagtgtcgaa ccgttcatca
    13621 cgatcatggt gccagcccat aatgaagaaa ttgtgatcga atatacaatc gagtatttga
    13681 tgacgaaatt gaattatcac aactatgagg tattagtgac agacgatggt tcaacggacc
    13741 aaacaccaga aatattagct agactgatga aaaaatacgc taatcttcgt gtggttcgga
    13801 tcgaaaaaaa taaaggaaaa gcccacgcgt ttaatatcgg acttgccttt gcaaaaggga
    13861 aattgatttt aagcaatgat gcagacacgg taccggagcc agatgcactg attaggtatg
    13921 tgaattattt cattcgccct ggtgcaagac atattgctgc ggtcactgca aatatggatg
    13981 tccaaaatcg taccaaattg attgcaaaat ctcaaacggt tgaattctca agtatcgtgg
    14041 gcatcattaa acgaacacag tcagctgttt tcggtggtct ttatgcgtac agtggagcaa
    14101 atacgatgta tcgaaaagaa gccttgatcg atgtgggcgg attcagacaa gatcgagcga
    14161 cagaagacat cagtattgct tgggatcatc agttaaatga ctggttatct gtttttgctc
    14221 ctgagattat ctttttcatg gaagtacctg tgacgttgaa gatgctatat cgacacagaa
    14281 aacgttgggc aaaaggtgga acagaagtct ggctgacgaa tttcaaaaaa gtgatgctcc
    14341 atccttttaa acatattgga agaacgatca tcttcattga tcaaacattg agtattgtct
    14401 ggtcaatatt cttttgtatt tccgtagttc tttttgctgg attaatcggg cattatgtat
    14461 atcaaggaaa ctacgaacag atttatatta cttttacatt cagttttgtg ttcatctgct
    14521 ttgaaatggt cgcaggattt ttccagttgc tagcttcgct gattgtagat gatcgacgaa
    14581 gaaaattgaa ataccttctt ttcgcaccac tatatatgct tttgttctgg atagtgaacg
    14641 cgataacgat cgtgacgact ttcattccag cagtcaaaac gattttaggt tatggaagcg
    14701 ggacatggaa aagcccggaa cgtacaaaaa aataaaagat gcaccgtttt ttagacaaac
    14761 agcctgttgt gtttatggtt cttccaaaca tttttgctta taataagaaa ttagttaaaa
    14821 agtagtaagg atgggaaaag tgatgaaaaa aagaatgaag gcgttaatct ggccaattgc
    14881 tgtttccagt attttcttat tgggacagac agcttttgcg gaaacttctg aacctgctca
    14941 aacagaacct tcaacaacca taagctcttc tggtgctgaa aacggtgcct cggaaacaga
    15001 aatgacttcc aatgtaccga cagataattc aggagaaacg atttcatctt cagaagatac
    15061 atcaataaat agttctacct cttctgacca aatcgatcat ttgcaagact tggaaaataa
    15121 aagaaaagaa ctcgaagaag cattagaagc tgcaaaaaac aaattgaaag aagaccaagc
    15181 agcaatcgat caagatttag ctgatcttaa aaataaagtg ggaaatgacc gtgcacaact
    15241 tgagaaaaag cttgccgaag cgaaagcaaa agcggaagct gtccaaacag atcttgacgg
    15301 gaagatccaa caagcaaaag aattcaatca gcaattaaat caggttgctc aaacagcttc
    15361 tggaaagcta agtcaaactc aagcggatat cacagcggcc agtcaagcaa tccacggaaa
    15421 gatcagcgaa gcacaaacag ttgctaatga ttttgaagca aaaaaagctg acttagcagc
    15481 tagtttccaa cgaataaaag atattttgca ttcggcagga gaaaaatcag tgacctctgg
    15541 aagcacgaca gaaacagttc agcaaacgac agagccatcc aaatcaacaa gtcagacttt
    15601 gccaaaaaca aatgaaaaaa cagcttctgt cagttattcc ttagcagggg cgggtgtgtt
    15661 agtcgcagca gcttacggaa tcatccgcaa aaagaaataa aaaagagagg atgggaaggt
    15721 agagtacctc gaagttagac tgaaaaatct aattcttgag tgccactaca tgtcccatct
    15781 ctctttagtt attaaaacag atgtccccta cttatctcgt ggagacagct gttccactac
    15841 atgtgaccat cagcatgcca ttgtcgctgc ctaatacttc atagtccatt tttacgccaa
    15901 tgatcgcatc agctcctaat gcttttgcac gttcggccat ttcggcaagt gcttcttctc
    15961 ttgcacccat tagttcgttt tcatagcttt ttgagcggcc gccaaagata tttcgcaacc
    16021 ccgcctccaa gtctttaaat gcatggatcc cagtaatgac ctcaccaaaa acgatccctt
    16081 tataagctac tacggctttt ccctctacac tgttcgttgt tgttgtaatc attcgatcac
    16141 tcctctcttt taattaaagt ataacaaact tcaagaagca ctacacatat tttcccaaaa
    16201 ttagaaattt attatatact tgaattgttc attaatgaaa gcgaaaccaa tcgttagcaa
    16261 ctatgtaaaa gaaaggaaga ttcttgtgaa aaaaagtatt ttgcttattt ctcatagtca
    16321 aaaagtaaca gatggtatta aagaaatgat tgagcagatg caaaaatccg aagaggtgca
    16381 aattttttct ctcggcggaa taaatggcga gatcggatca gacccgatga aaatcgtaga
    16441 agcagtcaat gaatcaagcg atgcagctag ttattttgtt tttgcggata tcggtagcgc
    16501 ggtattgaac tcggaactgg caaaagacat gttggaagaa gagcagcagc aacgttatca
    16561 tctggtggat gcgcctttag tggaaggagc ttttgcagca gccatcactg ccggtataac
    16621 agatgatact cagcaaatct taacagaagc acaaaatgcc ggaaagaaag gatgggaata
    16681 aattgaaaaa aatcatgaat gatccaagca atattgtcga agaaatgctg gaaggattag
    16741 taaaaagcta tccagaactt gttcatcgtg tagaatcttc ccgtgttgta gccaagaatc
    16801 agaaagcgga acaagttggt ttggtttctg gtggaggaag cggacatgaa ccatcccatg
    16861 ccgggtttgt aggggaaggg atgttaagtg cagcggtgct tggtgatgtg tttacttctc
    16921 caactccaga tcagattcag acggctatta aggaagccga tagtggaaaa ggagttttgc
    16981 tgatcgttaa gaattataca ggggatgcat tgaacttcga tatggcaaag gaattagctg
    17041 ctatggacga tattgaagta gaaagtgtga tcgtagatga tgatatcgcc gttgaaaata
    17101 gtacgtatac tgctggaaaa cgcggagttg caggtactgt ccttgtacac aaaatcgtgg
    17161 gcgatgcagc aagaaacggt gcttccctag ctgagttgaa agaactggga gaaaaggtcg
    17221 tacaggcaac taagacgatc ggtctagcgc ttcgagcagc aactgtacca gaagtaggga
    17281 aaccaggatt cgaactagga gaagatgaga tcgagtatgg tgttggtatc cacggtgagc
    17341 caggctatcg tcgagaaaag atgcagcctt ctaaagtact ggcaaaggaa cttgttacta
    17401 agattttaga tgattactcg aatttaccaa aagaagcagg tgtactagtc aacggaatgg
    17461 gcgggacgcc tttgatggaa cagttcgtgt tcatgaatga tgttttagct ctgttggaag
    17521 aaagaggcgt caatgtagtt ttccgaaaag tcggtaattt tatgacctca ctagatatgc
    17581 aaggtctttc tctaacactg atcgacttga cggagacaca atggaaagat tctttagaaa
    17641 gcaatgtaca gacgatttca tggtaaaaaa tctcaagata aaagggtgat aatatgaaac
    17701 taaccgttca agaaatccaa gaatggctca cgcaatttgc agaggcaatc aatcagaaca
    17761 aacaatatct cagtgatcta gataccccga ttggcgatgg agaccatgga aataacatgg
    17821 gaagaggggt ttcagcttat gaagaagctt ttcaagccga tcatcctgaa acaatcagcg
    17881 atacattcaa agtattctct atggcgatga tatcgaaagt cggtggggct tctggtccgt
    17941 tatacggatc tgcattcatg aacatgatga aagcgacaaa agaggtcgat tctattgatt
    18001 ctcaagaaaa attaggcgaa gtgatcgagc agggaacagc gggtattcaa agccgaggaa
    18061 aggcagaagc tgacgataaa acgatgttag atgtttggct gccagtcaca gaggcattga
    18121 aggcagggaa tttaacaaaa gaaatcattg accaggcaaa agaacacacc aaagtcttgg
    18181 tagctaaaaa aggacgagct tcctatctag gagagcgtgc aatcggacat atcgacccag
    18241 gagctgcttc tagtgccatt ttattttcaa cgcttttaga tgtgatcaat caaccttaag
    18301 ttcaataaaa ctgcggtgat gctcatcgca gttttttgtt tggctgttct tatgacttga
    18361 tgcagaacag ctttttcaca acctctattt gtccatatcg caaaattcag gtagaataca
    18421 aaagataacg gaaagaggcg aaacaatgga acttggaaaa tcagattatc agttgtttga
    18481 tcgaccaatc tacgcattca aacagctcaa agagagccat ccaacagata agattgaaca
    18541 aatcaagaaa gaatataaag aacactggca aaaatggaaa gagattcagc tccagacagc
    18601 tgcacttttg cctgatatgt acggcatgag caaacctaaa atcgaaagct ggacgaatgg
    18661 ctggaattta cgcagtcatt tttggagcgc gtaccgaagc gaatctcgtc aagacgagaa
    18721 tgcctgtcta gcagttttgt tgaatcaaaa acaataccaa atctatttga tgtaccaaca
    18781 ttataaaagc gaagaacgat atggtagcat tgcagcgtat aaccagttgc ttgcgatact
    18841 gaaagagtgg agcaaaacag tcgatatcaa ggattattac atatggccgc aaccagaaca
    18901 tgaacttgac gaccacttag ctttgtctga ctatttgtcc gatacaaaga aacaagaaga
    18961 gttgaaaaaa gcgatgagag accgaacctt tcaaatgggt aaactattct tctatccgca
    19021 agaaatcgaa cacgttgagc aaataaccgc tgaaacctta caagaactag caccactata
    19081 tcaaaagcta ggtgcggaaa aatttcaata gacaacacga aataagcaat gcgaaaaaca
    19141 aaccttactc tcggcttgcg agtgaaggtt tgtttttgat tattcaaaaa gtaaatattt
    19201 aaaaaattga taggaatcaa ttacttaagg tctcaaaaga aggaaaataa agcaagggac
    19261 aaaaagaaat ccactctatt tgctggtatc tggttcgctt gacgtttatg ctatgataaa
    19321 agaaaattat cagttggaat aagggaagtt atgtttcttt attagaaata gctgtttaga
    19381 aggaggagag attatgagtc atgatcattt atgtgtttca tttgttcctt tgttcaatca
    19441 cttagtacag caagatcaag aaaaagtaca cgcacttgtc aaacatcact atgtggaaaa
    19501 aggagaacag attcttgcgc cgaatggaga acctcaatta gtgatcgtgg cctccggcag
    19561 tatgaaagtg tatcaattat ctgctagcgg aaaggaacag cttttacgag taatagagcc
    19621 gggcggatat gagggagaga gccagttgct aggcgttcca aatgagtctt tatttggcga
    19681 agcactagaa aaaacggaaa tttgcatatt gagacaaaaa gatttctcgg aactcttact
    19741 gcattatccc gcccttagtc tgaaactttt agcaatcaac gttgagaaat taacaaaagt
    19801 acagcagcag acaacttttt tgatgatgga aaaagtagaa gaacgagtcg ctgtctatct
    19861 gcttgactta gctaaagcga cagggaagga tcactttttc ctgccaatga aaatgaaaga
    19921 acttgctgca tttatcggta cgacccccga aactttatct agaaaattta aattattaga
    19981 ggagaatggt tatgtaaaaa gaaagaatcg agaagtctgg atcattgata aagagcagtt
    20041 agaagacttg taaataaaaa aatgacctcc aaatgttgga tttttgaccc agatttggag
    20101 gctagcatga aattgtacat gcttttttat tatccttcta gtaagtctct tcgcctataa
    20161 ccaatagaac caaggacgaa cagtgcaagg ctgatacatg ataacgtaag gaacacggaa
    20221 agatcaaaag aatcgtttgg catccgtgga acccagctag ggacagctgt tttggaaaac
    20281 cattctggta aatccaaaat tccgccaaag taattgagtg caaaggagta agctaagtag
    20341 atataagcaa gtttaccgat tcgaggaagc catccgaata gaaagccact aattccgata
    20401 aacactaaaa cggcaggcag gaaattgaac ccggctttaa gaaacatcaa tagatccatg
    20461 tctttcgcat ccttcatcga agccaaagca gaaagcccta gtgcaccaga actaatcagg
    20521 attgccaata tagcaaatag aatagctagt aacatattgg tccaataaag ttggttccta
    20581 gaaatttttg ttgcgaacag ctgattcata cgtccgtggg tttcttctgt aaataaacga
    20641 ttgatgctag caattggtaa aatagcagct agacaaatca agacgaccat gatcgtgctt
    20701 gtaaagtttt tttcaattga gacgccagat tggataaaca tctgtttcat taagtcgtta
    20761 ctttctaaaa actgttgcat attcccgtag atagagccgt atgccgcccc catcaaacca
    20821 aaagtgataa gccaactgat tgtcatccct ttatttagat agaataataa accacgcaag
    20881 gataaaagag aacgtttagc atacggtttt ccttcacgtt cggggatata gccggtgttc
    20941 aaatctcgat gttcctctaa aacaaatgaa ctgattatca agattagact gaacaaaaac
    21001 aaataaagca aaggaatcca gttgttagtt gtaaatggat aggtcaaata agaccagcct
    21061 aaaggattcc acatcgaaag ggaggaatca gctacatctg ttattgctcg tagaaggtaa
    21121 agtccaccta aaaacgctaa agacagacca ttcgaactgg ctgaacttgg catcagttgt
    21181 gcagcaaaca gtgcaacggc actccccaaa atccccatac tacctacaga tagcccaaaa
    21241 agccaagcac cttctgcagt gatcgaatcc gcttggaaaa aaatcatcgt acagctgatc
    21301 gatattgcta aaagcaaatt gatcagaata ctttcagcga tgacagctaa agagtttgct
    21361 tgacgtccga ctcgaaagga acgaataagt tcaatcagac caagttcttc tttttttctc
    21421 gtttggctca ccacgtgtaa aacagagatc acagcagcaa acaaactgca aaaaagcaac
    21481 atttcatttg catacatcgc tcctaaagta tactcagcag cattcgaagc tggagttggt
    21541 ccgaccattg caatcattgc cgggttttgc aaggtttcgt ataatccagc taatccttgt
    21601 ccggaagtca attcttcaaa cgcagggaca aaaccaccag aaaaagcagt gacaccgact
    21661 agccatatca ggatcttttt ccagtcacgg ctaaggtttt gagaaaaaag ggtggaccag
    21721 cgtgaaaatt tatcagtcat ttcgcttcct cctcatcctt cataatgtct catgaataaa
    21781 tcctcaagag tcggtggaat agattcaaat tttttcacac ctaatttgga cgcttcagct
    21841 aaaatcacat cgaaagcttc gttatcagca gcaaattgag cgaggtgacc tttttgcaca
    21901 aagtcatgga caccgttcaa ttgtgctaag tgactattct ctgcttctgt ttctatagtg
    21961 atggtcgaac gagtcaaatg acgcaattca tcgagtgttc cttcttcatc gagtgttcct
    22021 tcgcgtatga tcacgacttt atccgctagt cgctcgactt cactgagaat atgagaagaa
    22081 agtaggatgg atttgccctg ttctttcaat ttttcaactt cttcttggaa aatcgtttcc
    22141 atcaaaggat ctaaaccaga agtaggctca tcaaataaat agagatccga atcaacagcc
    22201 aaagcagcaa tcaagcccac tttttggcga tttccttttg aatagttttt tgcttttttc
    22261 ttaggatcaa gagagaaacg ttgaatcagt tcatcttttt tttgagcact cccttttcca
    22321 tgaagcttca tgaagagatc gatgatcgct cctccagtca aatttcccca caaagagata
    22381 tcgcctggaa cataagaaag acgttcatgg atagataaac tatcttccca tacatcttgc
    22441 ccaaaaatcg ttgccgttcc gccgtctttt tttataatcc ctaaaagtgt ccgaatcgtc
    22501 gtggattttc cagaaccatt tggtccgata aatccaacga cttcacctga atgtacggtg
    22561 aaagagacat cttttaaagc ttcgaaacga ccaaagctct ttttcaaatg tgtgactttg
    22621 acgatttctg ccataaaaa
//
LOCUS       DAZKLD010000035        22337 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.035, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000035 DAZKLD010000000
VERSION     DAZKLD010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22337
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.035"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..930
                     /locus_tag="ACG8KR_001095"
     CDS             <1..930
                     /locus_tag="ACG8KR_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rib/alpha-like domain-containing protein"
                     /protein_id="HFQ0200482.1"
                     /translation="DKYNPEGQKVTTDLNKEPDASEGIKNKEDLPKGTTYAWKEKVDV
                     STAGNKKGTIVVTYPDGSSDEVEVDVTVTDTRSDADKYNPEGQKVTTDLNKEPDASEG
                     IKNKEDLPKGTTYTWKEKVDVSTAGNKKGTVVVTYPDGSKEEVEVTISVVDKKAPNKP
                     QVDPITEGDQIVTGKTEPNAEVTVTLPDGSQHHGTADKNGNFTVKVPKLEAGTKVIVT
                     ATDESGNTSEPTNVVVSSNKKDSGKNGSKGSKTDNQGSNSNQDKNRGKSQSSKVFPKT
                     GESDSNIFTISGGLILLGTLGLLGYKNRKKENE"
     gene            1064..2407
                     /locus_tag="ACG8KR_001096"
     CDS             1064..2407
                     /locus_tag="ACG8KR_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFQ0200483.1"
                     /translation="MEKIVIIGSNHSGLAAARFLNLSDSNYEVILIDKKSEISYLECG
                     TSLFIKGEVSNLEDLFYQNRMTLNSEIKKLFLETEVDKIDIQNKKISCVKKDGTRFSQ
                     EYDKVILATGSKQIMLDIPGNNLANIFSIKNFPNAKEIFKALDDSTIKKVGIIGAGYI
                     GVEIAEAIRKRGKEVYLLDVADRVLSTYYDRSFSDKVEEILSKNGIHLCLSESPKKYL
                     GRKKIEKIVTTSGTQYSMDMVVQAIGFLSNSPIGEKELLRDVSGAYLTNEYQQTNIKD
                     IYAIGDCATTFFTSINRSSVDFSISNALRTAYIATQHINNQDFTPSGSQFTNGFSIFN
                     YNFYAAGLNLKTARLLSESIDYIEIEDFIRPAFLRKNSRVKLRISFEKNTKRIVGVQL
                     CSQEDLSGLLSMFSLAIEEQITLDKLKMLDYLFHPSFSQPYNFILKAVTGLNIET"
     gene            2519..3922
                     /locus_tag="ACG8KR_001097"
     CDS             2519..3922
                     /locus_tag="ACG8KR_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFQ0200484.1"
                     /translation="MKKSLKLASAVILMSQLLIATPFHVLASENSTYKIAKERTETTE
                     MSNSDVTAESSELLTTSSSESSTTSNAESSTTSSSESSTTSNAESSTTSSSESSTISS
                     TESSTTSSSTSEITSSKVTKPNHIESSKKEAPKTEGQTKTFSTPQQIESTESFQSQPI
                     LSGNEEIHFDKNESVENFIMKIGESARKIGQKYDLYASVMIAQAILESASGQSQLAQA
                     PNYNLFGIKGTYNGNFVIMVTNEDLGNGTLYTTQSKFRVYENYEESFEDYAKLLTKGI
                     SGNKDFYAGALKANSKTYREATKFLTGRYATDTQYYLKLNELIKTYDLTNYDKEVKGP
                     QMSKTGYQVPIHNYSITSLFGNRGNEFHRGLDMAATQGEPIHAVKAGKVVKAEFHYSW
                     GNYVVIQHEDGSTALYAHQQQYLVKTGDNVLQGQIIGYVGSTGNSTGPHLHLEICKDS
                     SLSQSMLVDPKTLLFGD"
     gene            4271..4903
                     /locus_tag="ACG8KR_001098"
     CDS             4271..4903
                     /locus_tag="ACG8KR_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AP2 domain-containing protein"
                     /protein_id="HFQ0200485.1"
                     /translation="MKERYGHLTVVEEITHNGYKKYRCLCDCGNETIVYRSNLLSGRT
                     KSCGCGNYKNRHKYKNLTNKTFGKLVAIKPTKLRSEGTVVWECQCLCGRKKLVSAKRL
                     LKGDVKSCGCLKSKKHHIENKKFGLLTAKYPLDNNCSWKTKWVCHCECGSMCEISYSN
                     LFFGHTQSCGCLKRQETRTCVEGTVIECLTSKLPKNNTSGVKGVYRSKEK"
     gene            5491..6930
                     /locus_tag="ACG8KR_001099"
     CDS             5491..6930
                     /locus_tag="ACG8KR_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFQ0200486.1"
                     /translation="MYSYQEDAKVQKNRWWILIAISMFTFMSTLDGSIVNIALPTISD
                     DMGIPMNQAEWVVSVYLMIVCSCLLLFGKIGDSRGKIKVFRIGTLIFVLGSLLCGFNH
                     SLGILLFARVVQAIGASMTMATNAGIITEVFPIHERGRALGAIGAFVSLGSIAGPGLG
                     GLILSQFSWAYIFWINVPVGIVTMLIAEKFLPKDITMSGHTIDGGGFAAIAATIISFF
                     GAIFLGQESGFFHPVPLVLFVISVIAFVSFLKVEKKAASPLISFGIFKNKIFTMSLIT
                     ALLIFSSNFFVNVVIPFYLQNARGLSPSYAGLLMMVFPLMMVVGAPLSGYLTDRVGPE
                     ILVLVGLGLLSVTQVMYMFLSVDSPLWLYIVATAIMGAGNALFQSPNNTMVMSSVTIE
                     NLGLAGSMNSFARNLGMVSGISLSTTVLYQAMSAKMGQQVTNYIANRPDVFLYGMKIT
                     FLGSFVLCFVAFFLTIWRIKKQGGIQKNV"
     gene            7225..7443
                     /locus_tag="ACG8KR_001100"
                     /pseudo
     CDS             7225..7443
                     /locus_tag="ACG8KR_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
     gene            7692..7907
                     /locus_tag="ACG8KR_001101"
     CDS             7692..7907
                     /locus_tag="ACG8KR_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0200487.1"
                     /translation="MNAGIADMNLIKKTLNDFTSNSISKGTGINLSTIKKLKSGERSV
                     EKLNLLDAIKITEFAMKNGKAEIEIWR"
     gene            complement(8124..8380)
                     /locus_tag="ACG8KR_001102"
                     /pseudo
     CDS             complement(8124..8380)
                     /locus_tag="ACG8KR_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(8567..9478)
                     /locus_tag="ACG8KR_001103"
     CDS             complement(8567..9478)
                     /locus_tag="ACG8KR_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFQ0200488.1"
                     /translation="MKIKIERNQKEKSPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GVYKNFTAIDQHTTHEKVVIKEKVVNTSGVESFTEDFAKEYFSWKNNKEAIEKRMSNL
                     EQYLTENGLALIQDMVRADIPTSSEVQSVKILDVEKHSEEFVVSFLVEQHITEGKKTQ
                     SISSAYRVTIFEDENRNHLVTSLPTMIGKPDKAKYEAKQVENDSEIDAKTTEEITDFL
                     ETFFKLYPSASEKELEYYVENGAMRPINIDLKFSELENPVYLQNGKNFLVSTKIKYLD
                     NVSKTTSYFQYKLVLQKNGNWKIVDVK"
     gene            complement(9497..10501)
                     /locus_tag="ACG8KR_001104"
     CDS             complement(9497..10501)
                     /locus_tag="ACG8KR_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HFQ0200489.1"
                     /translation="MNLKKVGVISLTFLTISFMGILMCVGLLFGEDSEGNGSSGTSPG
                     GTSVSEEVLKHRAMVEKYAKESDITEYIPILLAIIQVESGGTMEDVMQSSESAGLPPN
                     SLSTEASIKQGCLYFASLVKRSKELGCDQDSIIQAYNYGGGYLDHVGKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENGGWRYNYGNMFYCLLVKQYLTTTQFDDKIVQEIF
                     DEAFKYEGTAYVFGGSSPETGFDCSGLTQWCYAKVDLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLVFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTENYWRQHLIGAGRYK
                     "
     gene            complement(10498..12621)
                     /locus_tag="ACG8KR_001105"
     CDS             complement(10498..12621)
                     /locus_tag="ACG8KR_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD3337/EF1877 family mobilome membrane protein"
                     /protein_id="HFQ0200490.1"
                     /translation="MKKKLFRIVGIVFISILTLIVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAESIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRAVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALTLGTEIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEDRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKKAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRALAFGGGAAVGNKLAKEQSKSKFKPSGSSLRKNSRLPNDHEILSDLTKEN
                     PVSNNKKQSRMNLAGRKIGKVLDTQALVKDKTKQVKDQVRNTPTNLKYNLHKGIEKSK
                     KAPEEFKRGLFQEKADRVDLREKQRQRRNAKMDEKRKILSEATNRQGKRRANIPIDKA
                     AQPQKDMMLKKEAPKRIVRANLNPEIKRKLTSQEIISKGKSQSASKKNSFQQVKQRST
                     LPKQTNQKVQKRMNHRPKSRSGEKK"
     gene            complement(12626..15073)
                     /locus_tag="ACG8KR_001106"
     CDS             complement(12626..15073)
                     /locus_tag="ACG8KR_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFQ0200491.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELIPYNYSFLSPEQKYQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLTEVAKQRIDLQTEALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKNLKKSFFSGFQEFVYGVNHHLMGDFVSLSNEEI
                     RRYTKLEKLMESKLARRFKVRRVTPSDLMYLIEHIYGEKGTPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPSRCLIEEKPRYLCMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKDSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWKE
                     TLPEIADEINILNLTSKPKNQGLLDPYVIMEKKKDSESLAIDILTFLTGISSRDGEKF
                     PVLRKAIRTVTQSNQRGLLQVINELRKEGSPVAENIADHIESFTDYDFAHLLFSDGSI
                     TQSISLDRQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNREI
                     FKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGLK
                     FAFRSTDIHEIKKTLEFFGLDREDESNQKRLRDLENGQCLMQDLYGRVGVVQVHPVFE
                     ELFHAFDTRPPVQERME"
     gene            complement(15060..15449)
                     /locus_tag="ACG8KR_001107"
     CDS             complement(15060..15449)
                     /locus_tag="ACG8KR_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFQ0200492.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEGAFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPITLVRSVNYVPD"
     gene            complement(15509..16012)
                     /locus_tag="ACG8KR_001108"
     CDS             complement(15509..16012)
                     /locus_tag="ACG8KR_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HFQ0200493.1"
                     /translation="MEQMRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELEGTPVGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCDDMSDVAYFLIVEAGDFGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFEYPY"
     gene            complement(16025..16249)
                     /locus_tag="ACG8KR_001109"
     CDS             complement(16025..16249)
                     /locus_tag="ACG8KR_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0200494.1"
                     /translation="MSGTYLSIYQWFLTNAQSLVLLAIVVIGLFLGFKREFSKLIGFL
                     VIALIAVGLVFNASGVKDVLLSLFNRIVGA"
     gene            complement(16353..18263)
                     /gene="ltrA"
                     /locus_tag="ACG8KR_001110"
     CDS             complement(16353..18263)
                     /gene="ltrA"
                     /locus_tag="ACG8KR_001110"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HFQ0200495.1"
                     /translation="MPKNKNETLCVEDLRHAEYYEMQNTFDDLYAKSKNGDIFTHLMD
                     IILSRENILLAYRNIKANAGSKTAGTDGTIIKDIGKLPAETVVKKVRYIVAGSPHGYR
                     PKPVRRKEIPKPNGKTRPLGIPCMWDRLIQQCIKQVLEPICEAKFSENSYGFRPNRSV
                     ENAIKATYNRLQISQLHYVIEFDIKGFFDNVNHSKLIKQIWAMGIRDKHLIFILKRIL
                     KAPIKMTNGTITYPEKGTPQGGIISPLLANIVLNELDHWVESQWQENPVTKNYVVHIN
                     KSGSPCKSNAYKEMKKTKLKEMYMVRYADDFRVFCRYKESAEKAKIAITQWIEQRLKL
                     EVSQEKTRIVNVRKRYSDFLGFKIKMIPRRKKLVVKSHISDKQFKKQKDKLVSQAKKI
                     ASPSKGKTELEETRLYNSMVLGMQNYYQIATNVNLDCMKLNRAVMAIFTNRLGTNKRG
                     RLRKTGRQLTTKEATRYGKSKMLRYVAGEGEPIFPVGYIQHRKPMAKVYLANCYTLEG
                     RKFIHTNLSVDKLLMYQLMKLSLENRTIEYADNRISLFSAQHGKCAITLEEFQQANEI
                     HCHHIVPTGAGGNDDYKNLILVKEAVHRLIHAKTEETIQKYIALLKLNKVQLIRLNKY
                     RVLAGNQELNLI"
     gene            complement(19009..19146)
                     /locus_tag="ACG8KR_001111"
     CDS             complement(19009..19146)
                     /locus_tag="ACG8KR_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HFQ0200496.1"
                     /translation="MKRGIVMGIVKDIFLVFGGSMLGVTVMCLMNASAAADRAMEKKG
                     K"
     gene            complement(19143..20327)
                     /gene="mobT"
                     /locus_tag="ACG8KR_001112"
     CDS             complement(19143..20327)
                     /gene="mobT"
                     /locus_tag="ACG8KR_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MobT family relaxase"
                     /protein_id="HFQ0200497.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATACGLSRPYLNQIENGGV
                     TASIKTMRKIFDQLESFNPDLPLTLLFDYVRIRFPTTDARKIIQEILHLKFEYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPELTKKCQKEECISLFRTFKSYRSGELLKADEKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAFHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNRKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNRTTYIQDMVQNARLSDRHKKILEQQSMAIENL
                     II"
     gene            complement(20586..20876)
                     /locus_tag="ACG8KR_001113"
     CDS             complement(20586..20876)
                     /locus_tag="ACG8KR_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0200498.1"
                     /translation="MVYDELIGEIYWVIGKIQSDPELEEQLRRLKFDIRKNGVKVAGD
                     PYLMNEETDARIEINQVIAEFERIADQAKEPDIRQYLLEMKAELEVNGITDE"
     gene            complement(20866..22008)
                     /gene="dcm"
                     /locus_tag="ACG8KR_001114"
                     /pseudo
     CDS             complement(20866..22008)
                     /gene="dcm"
                     /locus_tag="ACG8KR_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613853.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
     gene            complement(22084..22248)
                     /locus_tag="ACG8KR_001115"
     CDS             complement(22084..22248)
                     /locus_tag="ACG8KR_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0200499.1"
                     /translation="MTEKEQVTKIVKKYNKSIADLSENATAKEFKTVIKYVADQANEK
                     QRKLVGLNKK"
     gene            complement(22241..>22337)
                     /locus_tag="ACG8KR_001116"
     CDS             complement(22241..>22337)
                     /locus_tag="ACG8KR_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HFQ0200500.1"
                     /translation="NISEVHYATDNLELFLPLGYSRKDLEVQLHD"
BASE COUNT         6826 a         4372 c         3970 g         7169 t
ORIGIN      
        1 gataaataca atcctgaagg acaaaaagtg actacagact taaataaaga gccagatgca
       61 tctgagggaa ttaagaacaa agaagattta ccaaaaggaa ctacttatgc ttggaaagag
      121 aaagtagatg ttagcacagc tggaaataaa aaaggtacta ttgtagtgac atatccagat
      181 ggatcatctg atgaagttga agtagacgtt acagtgacag atactcgttc cgatgcagat
      241 aaatataatc ctgaaggaca aaaagtgact acagacttaa ataaagagcc agatgcatct
      301 gagggaatta agaacaaaga agatctacca aaaggaacta cttatacttg gaaagaaaaa
      361 gtagatgtta gtacagctgg aaacaaaaaa ggtactgtag tagtaacata tccagatgga
      421 tctaaagaag aagtagaagt tactatttct gtagtagata aaaaagcacc gaataaacct
      481 caagttgatc ctattacaga gggtgaccaa attgttactg gtaaaactga accaaatgca
      541 gaggtgacag ttacattacc agacggaagt caacaccatg ggacagctga taaaaatggt
      601 aattttacag ttaaagttcc taaattagag gcagggacaa aagtgatagt aactgcaact
      661 gatgaatctg gtaatactag tgaacctact aatgtagttg tttcttccaa caaaaaagac
      721 agtggaaaaa acggtagtaa aggtagtaag actgacaatc aaggtagcaa cagcaatcaa
      781 gacaaaaata gaggcaaaag ccagtcctca aaagtttttc ctaagacggg agagagtgac
      841 agtaatatct ttactattag cggtggttta atactgttag gaacgttagg attattaggg
      901 tataaaaatc gaaaaaaaga gaatgaatag aaaatcaatt tcttaagtct tctctgagaa
      961 gaggaagaat ctttttcttc ctctttttgc ttttttatga tgggaaataa gtgttagtta
     1021 aagatgaata tactttttat agaaaggaaa agaatgtaat taagtggaaa aaatagtaat
     1081 tattggttca aaccattcag ggctagccgc agctcgattt ctaaacttaa gcgattcaaa
     1141 ttatgaagtt attctaatcg ataaaaaatc agaaattagt tacttggaat gtgggacatc
     1201 gttatttata aaaggagaag taagtaattt agaagatctt ttttatcaaa atagaatgac
     1261 actaaattct gagataaaaa agttatttct agagacagaa gttgacaaaa ttgatattca
     1321 aaataaaaag attagttgtg taaaaaaaga tggtacaaga ttctctcaag aatatgacaa
     1381 agttatatta gctacaggtt caaagcaaat tatgctagat ataccgggga ataatttagc
     1441 taatattttt tcgataaaga attttccaaa tgcaaaagag atttttaaag ctttggatga
     1501 tagtactatt aaaaaagtag gaattattgg agcaggatat attggagttg aaattgctga
     1561 agccataaga aaaagaggga aagaagtata tcttttagat gttgcagata gagtgttgtc
     1621 gacttattac gaccgtagtt tttcagataa agttgaagaa atactatcaa aaaatggaat
     1681 acatctttgt ctatcagaaa gtcctaaaaa atatttagga agaaaaaaga ttgaaaaaat
     1741 agtcactaca agtggtaccc aatactctat ggatatggtc gtacaagcta taggatttct
     1801 atctaatagt ccaattgggg aaaaggaact cttgagagat gttagtggag catatctaac
     1861 taatgaatac cagcaaacaa atataaaaga tatatatgct attggggatt gtgctacaac
     1921 tttttttacg tccattaata gaagtagtgt tgatttttct atttctaatg ctctaagaac
     1981 agcttatatt gcaactcaac atattaacaa tcaagatttt acaccttctg gttcccaatt
     2041 tacaaatggc ttttctatat ttaattacaa tttttacgca gcaggtttga atttaaaaac
     2101 agcacgatta ttaagtgagt ctatagatta tatagaaata gaagatttca ttagacctgc
     2161 ttttttgaga aaaaatagtc gtgtaaaatt aagaattagc tttgaaaaaa atacaaagag
     2221 aatagtaggt gtacaacttt gtagtcaaga agatttatct ggactattat caatgttttc
     2281 tcttgctata gaagaacaaa ttacattaga caaattgaaa atgttagatt atttatttca
     2341 tcctagtttt agccaaccct ataattttat tttaaaggca gtaacaggat taaacattga
     2401 aacataatcg ttactaaatg aaatcaagaa atatactgta ttactcaatt cattgaaaat
     2461 atttatcata atttttaata gaaaacacta ttaaagagaa aaaagagagg taaatcaaat
     2521 gaaaaaatct ttaaaacttg catcagcagt tatattaatg tcccaactcc ttatagcaac
     2581 acctttccat gtgttggcaa gcgaaaattc aacatacaaa attgcaaaag aaagaaccga
     2641 gacgactgaa atgagtaatt ccgatgtaac agcagagagt agtgaacttt tgacaacttc
     2701 atcaagtgaa agtagtacga cttcaaatgc cgagtcttca acaacttcat caagtgaaag
     2761 tagtacgact tcaaatgccg agtcttcaac aacttcgtca agtgaaagta gtacgatttc
     2821 aagtaccgaa tcttcaacaa cttcaagtag tacaagtgaa ataacttctt cgaaagtgac
     2881 aaaaccaaac catatagaat cttcaaaaaa ggaagcgcca aaaacggaag gacaaacaaa
     2941 aactttttcg acaccacagc aaatagaatc aacagaatct ttccaatcac aaccaatact
     3001 atctggtaat gaagaaattc attttgacaa aaatgaatct gtagaaaatt ttattatgaa
     3061 aattggtgag tctgcgagaa aaattggtca gaaatatgat ttatatgctt ctgttatgat
     3121 tgcacaagcg atcttagaat ctgccagtgg tcaaagccag ttagctcagg caccgaatta
     3181 caatttattt gggataaaag gaacttataa tggaaacttt gtcattatgg taacaaacga
     3241 agatttgggt aatggaacgc tttatacgac ccaatcaaaa tttagagtgt atgaaaacta
     3301 tgaggaaagc tttgaagatt atgccaaatt attaactaag ggaattagtg gaaataaaga
     3361 tttttatgca ggagcattaa aagctaatag taaaacatat cgagaagcaa ctaaattcct
     3421 tacaggacgg tatgcaacag atacacaata ctatttgaag ctaaatgaat taattaaaac
     3481 ctacgattta accaactatg ataaagaagt taaaggtcca caaatgagta agactggata
     3541 ccaagtgcct attcataatt attcaataac cagtttgttt ggaaatcgtg gaaatgaatt
     3601 tcaccgagga cttgatatgg cagctacaca gggtgagccg attcatgcag ttaaagctgg
     3661 taaggttgtt aaagcagagt ttcactactc ttggggaaat tacgtggtga tacaacacga
     3721 agatggatca accgcgttat atgctcacca gcaacaatac ctagtaaaaa ctggagataa
     3781 tgttttgcaa ggacaaataa ttggctatgt aggaagtact gggaatagta ctggcccaca
     3841 ccttcatttg gaaatttgca aagatagtag tctttctcag agcatgttag ttgatccgaa
     3901 aaccttatta ttcggagact aaaaaaggta atcaatctga caaagtaaca agaaaatggt
     3961 tggattctta gacctttgat caggatattg agactggaat tgatatctcg attacgatgg
     4021 gtttgacaaa taaaacaagt ccattatcga atatcgagtg tattattgcc gtctttatgc
     4081 tcatattttg agcaggtttc actagatgaa aacaatttat ctatcttgag gatttcgtgt
     4141 acgtatcgtt cagtcttgta ttgtaaatta gtcataaaat cagactaaga aaaattggaa
     4201 atattttttt ctgatttatt aaaagttaat tgaaattata tggtacttct ttcttgggag
     4261 ggtaggtgca gtgaaggaaa gatatggtca ccttactgtt gttgaggaga ttactcataa
     4321 tggatataaa aaatatcgtt gcctatgtga ctgtgggaat gaaacgattg tatatcgatc
     4381 aaacttacta tcaggtagaa ccaaaagttg tggatgtgga aattataaaa ataggcataa
     4441 atataaaaat ttgacaaata aaacttttgg taagttagtt gcaatcaagc caactaaact
     4501 gcgaagtgaa ggaactgttg tttgggaatg tcaatgtctg tgtgggagaa aaaagttagt
     4561 atcggcaaag cgacttctaa aaggtgatgt aaaaagctgt ggatgtttga aatcaaaaaa
     4621 gcatcacatt gaaaataaaa aattcggatt acttactgca aagtatcctt tggacaataa
     4681 ctgttcatgg aaaacaaaat gggtttgtca ttgtgagtgt ggcagtatgt gtgagataag
     4741 ttatagtaac ttattttttg gacatactca aagttgtgga tgtttaaaaa ggcaagaaac
     4801 aaggacatgt gttgaaggga cagtaataga gtgtctaacg tcaaaattac cgaaaaataa
     4861 tacaagtggt gttaagggag tttatcgttc taaagagaaa tagattgcgt atataacttt
     4921 aaaaaaaaag aggtactatt taggatcgta tgattctatt aagagtgcag ctcaagctag
     4981 aaaagatgct gaaaataaat tgtttcaacc tttgattgac aaatatcgag gtaatcatta
     5041 attattgtgg ataataatta acagtgatat ataaacatct ataatatatt attcatacta
     5101 ttgatattta gtataatcct atggaattga atatcatagc aatgtatttg aaaagcaaat
     5161 tggaaaacaa ataggtgcaa caccataaat atgaaaaaat tgggaaaaag tagaatggaa
     5221 atttgcttac gtctcacgag atagctcaca ctattaatct gaagaacaat gaagtcgact
     5281 tttagaaatt gctagaagaa agtaaagcga attatcacta gttactatag agtaagttca
     5341 aataaagcat ctccaactta catggttaca tgaggatatc aaatcgaaat tactcaagct
     5401 tcctatggct agagtcgcga gaagttgttg ggcagtagac ataggttata ttgttcttag
     5461 ctacacgatc attagaaagg aaatcctatt atgtatagtt atcaagaaga tgcaaaagtc
     5521 caaaaaaatc gctggtggat tttgattgct atatctatgt ttaccttcat gtccacattg
     5581 gatggcagta ttgtcaatat tgcactacct accatctcag atgatatggg cataccaatg
     5641 aatcaagcgg aatgggtagt gtccgtttat ctgatgattg tctgttcttg tttgttgctg
     5701 tttggtaaaa ttggcgatag ccgtgggaaa atcaaagtat ttcggattgg tacgctgatc
     5761 tttgtcttgg gatcgctgtt gtgtgggttc aatcattcgc taggtatttt attgttcgca
     5821 cgggtggtac aagccattgg tgctagtatg actatggcta caaatgccgg aatcatcacg
     5881 gaagtctttc ccatacatga acgtggtcgg gcattgggag caataggtgc gtttgtttca
     5941 ttaggctcga ttgctggacc aggtcttggc ggcttgattt tgtcccaatt ttcttgggcg
     6001 tacatttttt ggatcaatgt accggtggga atcgtaacga tgttgattgc tgaaaaattc
     6061 ttaccaaaag atatcaccat gagcggccat acaatcgatg gagggggctt tgctgccatt
     6121 gcggctacga ttatcagctt ttttggagca atctttttgg ggcaagaaag tggctttttc
     6181 catccagtcc cccttgtact gttcgtaatt tcagtgattg cctttgtcag ctttttaaaa
     6241 gttgaaaaga aagcagcctc tccattgatt tctttcggta tttttaagaa caagattttt
     6301 accatgagct tgattacagc cttgttgatt ttttcttcca acttctttgt caatgtagtg
     6361 attccttttt atctgcaaaa tgctcgcggg ttgagtccta gctatgctgg gctgttgatg
     6421 atggtctttc ctttgatgat ggtcgtcggg gcgcctttaa gcggctacct gaccgaccgg
     6481 gttggcccgg agattttggt attagtaggc ttgggcttat tgtcggtaac ccaggtcatg
     6541 tatatgtttt tgtcagtgga ctctcctttg tggctataca ttgtcgctac tgccatcatg
     6601 ggtgccggca atgccttgtt ccagtccccc aataatacga tggttatgag ctcggtcact
     6661 atcgaaaact tgggactggc aggctcgatg aattcctttg cccgaaattt ggggatggtc
     6721 agcgggattt cgctttctac gacggtctta tatcaagcga tgtctgctaa gatgggccag
     6781 caagtaacta actatatcgc aaatcggccg gatgtctttt tatatggaat gaagattacc
     6841 tttttaggca gctttgtttt gtgttttgtt gcctttttcc tgactatctg gcgtatcaaa
     6901 aaacaaggag gtattcagaa aaatgtctga aaaaaccact gttgaagaat ttttggaggc
     6961 ctgcgtatgg gtgagtggga tttccatgga tgatggaaga tgaagtgaac ttctaaaagc
     7021 cgaaaaactc aagtattatc ttcaaaatac tagtgctcaa tgagatgctt gatttgatca
     7081 caagaagact gcgaaagccg ctggcaaccg caagctaggt atcccaccga tggactacgt
     7141 tttagaggct actggttatg aatacggagc caacgctctc atctgcataa gaatacatca
     7201 cccagattat gtaaagattt tcgatacttc tgtcaaaaaa tcattaagat agataaatga
     7261 tttccgtcta gtcaaattgg ttcagaatgt agacacacag atggtaagaa gtcactcaca
     7321 attcgagaat ggacttgtcc tatttgccgt atacatcata atcgaaacgt caacgatagt
     7381 atcaatattt tgaccaaggg ctatgaatac aagcattgcc ttagaaaaca tcaagaacct
     7441 taggaacaac ggggatagct tggttaataa gagataccgc tgtttaggct tgcaagtata
     7501 ctcttttatc aagaattccg tggctttagt catgagaggc ttaatactat gaaaattttc
     7561 tttagcattt ttgatttagg ttccttttct aattataata agatgaccac tacagtaacc
     7621 tactgaaata tataaacagc cactgtagta ccattttcaa agtagtctat gagaggagaa
     7681 tcttcattct tatgaacgct ggaattgcgg atatgaattt aataaaaaaa acccttaatg
     7741 attttacttc caattcaata tcaaaaggta ctggtatcaa tttaagcaca ataaaaaagc
     7801 tgaaaagtgg tgaaagatca gtggaaaagc tgaatctttt agatgctata aaaataacgg
     7861 aatttgctat gaaaaatgga aaagctgaga ttgaaatatg gagataatta tcgtctagtg
     7921 aattacttgc cacttagctg aatttaacga tccttggtgc ttgaaggggg agattcttgg
     7981 gaatagagca tacttgcaag cctatgtctt tactaacaac gatatctctt attaaccaag
     8041 ctatccccgt agttctaagg cttttacttt tatctaagca ataatcgtat tcaaaggaaa
     8101 tttttgaccc ttagaaagtc tcattttatc agacgtgagc ttaggcattg ctgaattttg
     8161 ttaattcggt tttaggttgt gctttgagca taatatgaaa gtattcttta tcacgatttc
     8221 attcatctaa ggtaatgtga tgtgattctg aaattctttc aaaggcagtt tttgcataag
     8281 ctgacattac atcatcaatg acatgtttac aatgtttcac tactaaaata agggggtaac
     8341 caagaagaaa aactgaatga ttatttgtat ctaattccat ggtatatcaa ccatttccta
     8401 tatagactga ttatgacatg gattaaaata aaaaggcact atgtcttaat tgtcggtttt
     8461 cgagttaccg aatggcattc atcacccacc tatagaggat gggcgacttc tgcctgaaat
     8521 aagttacact acttataact tctaataaaa ttagattgag tcattattat ttaacatcaa
     8581 cgattttcca gtttccattt ttttgtaata ctaatttgta ctgaaagtag ctagtggttt
     8641 ttgatacatt atcaaggtat tttatctttg tactcactag aaaatttttt ccgttttgta
     8701 gataaactgg attctctaac tccgaaaact ttaagtcaat attgattggt cgcatcgcac
     8761 cattttcaac ataatactcc aattcttttt cagaagctga tggataaagt ttaaaaaatg
     8821 tttctaaaaa atcggtgatt tcttccgttg ttttggcatc aatttctgaa tcgttttcta
     8881 cttgttttgc ttcatatttt gccttgtctg gctttccaat cattgttggc aaactagtta
     8941 ctaaatgatt cctgttttca tcttcaaaga ttgtcactcg atatgcagaa gaaattgatt
     9001 gcgtcttttt tccctctgta atatgttgct ccactaaaaa tgaaacgaca aattcttccg
     9061 agtgtttttc aacatctaga atttttacag attgaacttc ggaactcgtg ggaatatctg
     9121 ctcgaaccat atcttggatt aaagcgagac cgttttctgt aaggtactgt tcgagattac
     9181 tcattctctt ttcaattgct tctttgttat ttttccatga aaaatattct ttcgcaaaat
     9241 cctctgtaaa actttcaacg ccagaagtat tcactacctt ctcttttata accacttttt
     9301 catgagttgt atgttgatca atggcggtga aatttttata aacgccaaaa gcaagactac
     9361 aagacaataa aagccaaagg gcaagcacca tttttcgatg cttacccacg gatacaactc
     9421 gttctttttt cttttttggg ctcttttcct tttgattccg ttcaattttt atcttcattc
     9481 tttttcctct tttctcttat ttgtatcggc cagctccgat aagatgctgc cgccaatagt
     9541 tttccgttag attcgtccac cccactggat ctcctgcatg aaacatgcga ttctcccctg
     9601 cgtaaatgcc cacatgggtc acataagtac cggcatcata ggttgaatga aagaaaacta
     9661 aatcacctgg tttggcatct tttagatcaa tatgagtcat ggcatcatat tgatcctgag
     9721 ccacccgagg aagtttcagg tcaactttgg cataacacca ttgggtcaag ccactacaat
     9781 caaaccccgt ttctggactt gagccaccaa acacataggc cgttccttca tacttaaatg
     9841 cctcatcaaa aatttcttgg actattttgt catcaaactg agttgtagtt aggtattgct
     9901 tcactaataa acagtaaaac atattgccat aattataccg ccacccacca ttttccttaa
     9961 tcgcgattgg gttggggtaa tcgacttttt gaccacctga tttttccttt gaaaaagatt
    10021 cagctaaagc aaaactatgc ttcttcccgt ttttcccgac atgatctaga tacccgccac
    10081 catagttata ggcctgaata atactatctt gatcacaccc tagttctttg gaacgcttaa
    10141 ccaaagacgc aaaatagaga cagccttgtt tgatggatgc ctcggtactt aatgaattag
    10201 gtggtaatcc ggctgactca gaactttgca taacgtcttc cattgtgcca ccagattcca
    10261 cttgaataat cgccaaaaga attggaatat actctgttat atccgattct ttggcatact
    10321 tttccaccat tgcacggtgt ttcaatactt cttctgagac actagttccg ccaggagaag
    10381 tgccagaact accatttccc tcactgtctt caccaaacaa caaacccaca cacatcaaaa
    10441 tacccataaa agagatggtc agaaaagtta atgaaataac acccactttc ttcaagttca
    10501 ttttttctca cctgaccttg attttggacg atggttcatt cttttttgta ctttttgatt
    10561 ggtctgcttt ggcagagtgg acctctgttt cacttgttgg aaagaatttt tcttacttgc
    10621 tgattggctt ttcccttttg aaatgatttc ttgactagtt agttttcgtt tgatttcggg
    10681 attcagattt gctctcacaa tccgctttgg tgcttctttt ttcagcatca tatctttctg
    10741 aggttgagct gctttatcta ttggaatatt cgcccttctt tttccctgac gatttgttgc
    10801 ttcactcagt atcttgcgtt tttcgtccat tttcgcattt cttcgttgtc gttgtttttc
    10861 tcgcaaatcc actcgatccg ctttttcttg gaacagtccg cgcttgaatt cttctggtgc
    10921 ttttttcgac ttttcgattc ctttatggag attgtatttc aaattcgttg gcgtattcct
    10981 tacctgatcc tttacttgtt tagtcttatc tttcaccaaa gcttgggtat ccaaaacttt
    11041 gccgattttt ctcccagcca aattcattcg ggattgtttc ttattatttg aaactggatt
    11101 ctccttagtt aagtcagata gtatttcgtg atcgttcggt aatcgtgaat tctttcgtag
    11161 agaagaaccg gatggcttaa atttggattt tgattgttcc tttgccaact tatttccgac
    11221 tgctgcacca ccaccaaaag ccaaagctcg tcccacattt cgattgagtt gccgcaattt
    11281 tcgattcatt aacatttgcg gttttcgcat gacgcgtctt cccattgact ggctatcatt
    11341 actctggagg ttgaacatac tcataatgtc gcccaatttg aaatagatac cggcaaaggt
    11401 cacaatttgc agaaaagcaa ccatgaaaaa tggataggtt gccgaaatat tgaaaaacat
    11461 cgtggagatg ctaaatgcag tcgtaatcac caaagtaaca cccgctcgca tcataatcgt
    11521 attgaataaa cgaataatcg ccttctttcc taaactttca taagtcggca acatggacaa
    11581 taagaaacta attggcagaa acatcgcaaa gataatgaag agaatttgcg agaacaacat
    11641 aatccctgtt agaagaaaaa caaagaagga aatgccaata ttgaacaata ccaaaaagac
    11701 cacggtccct aaacggttca tggtcttggt aatactgaga ttcgcattgt cattgtcttc
    11761 aatctctgcc ttgaccgctt cttcccgatc tttgcctttg ttttcatctg gactcacaga
    11821 tagaatcttg ttgacccggt cttcaccaat ttcttctatg ttggaatcat caaattgcag
    11881 tagtaaccaa ggttgttgga cttgaatcga aaacaaacta tcccgaatca aatccacact
    11941 atcttttcct tggctttctg aattgggtac aacgatctcg gttccaaggg taagggccgc
    12001 ttcactaaca tctgagctaa agtcattgat ttttgtgata taggtaggcg cataagcaat
    12061 gaacgaaccc gatagtagaa aaatcatcac aaaattaatc acggctcgaa ctgctttcgt
    12121 ggtttctcgt tttaataatc cggtatacgc cacatacacc cccagagcta aaatcatcaa
    12181 taatagaaac ccaaaataaa atccggaggt ctggagtcca ttttcagtaa taccagccaa
    12241 tgtttggata ttctttccaa tactttcagc cgtatccgaa atgaaatcta atttgtaagc
    12301 ctcttgaacc acataacccg tggcgttcga caaatacaaa ctcaccgtcc aaatgaaatt
    12361 agtaatggca taaagaccat acatcacgct tttccctaag ccatcacccc aattccaagg
    12421 caaccaatcc caagagctat ccacaaagaa atctagttga tagttgttca gtgaatattt
    12481 ggaatataga tttcctgctt caactgtatc atcaactagc cctgtcgcct cggctaccgt
    12541 accaactaaa ctcaatagaa ctatcagagt gagtatgcta ataaaaacga ttcccacaat
    12601 ccgaaaaagt ttctttttca tcccctcact ccattctctc ttgaacaggt ggacgggtat
    12661 caaaggcatg gaagagttct tcaaagactg gatgtacctg aaccacgcct acccgaccat
    12721 acaaatcctg catcaagcat tggccatttt ccaaatctcg taggcgtttt tgattgcttt
    12781 catcttctcg atccaagcca aagaattcca aggttttctt gatttcatga atatcggtac
    12841 tgcgaaacgc aaacttcaac ccgatattat tcttcaagcg ttcatccagt aaatcatcac
    12901 tattttgcgt gacaaagtaa accccggcat tcatagcccg cccagcacga acgagcttat
    12961 tcgataatgt tttcccttgc gctacttgaa gaaaactcca cgcctcatct aagtctacaa
    13021 ttttgaagat ttctcgatta ctatggataa agtccaaggc aaaagtggaa atgacgatta
    13081 gcatggaaac actaagcaat tccatggtgg tatattcttc aaaagtggtt tctgcatctg
    13141 gtaaaaccaa atccgctact tgaatgatat ttaactgcct atctaaacta atcgactggg
    13201 taatcgaacc atctgagaaa agcaaatggg caaaatcata atcggtaaag gactcgatgt
    13261 gatccgcgat attttccgca actggtgagc cttcttttcg aagctcatta attacttgta
    13321 ataatccccg ttgattgctt tgggtaaccg tccgaatggc cttacgcaac actggaaact
    13381 tctccccatc tcgactagaa attcctgtta aaaaggtcag aatatcaatt gctagacttt
    13441 cagaatcctt ctttttctcc ataatcacat aaggatcaag taatccttga ttttttggct
    13501 tactagttaa gttgagaata ttgatttcat ccgcaatttc tggcaaggtt tctttccagc
    13561 caccccgttc cccttttgga tcgacaatca cagcttgtcc accaaaaagg acggcgtaat
    13621 aaactaagag attgttacta aaggactttc cgccaccgag tgaaccgagg aatgccgctg
    13681 ctagcgcatt ggtaactgaa tccttgaccc cttgtgaggc caatgccggt ttcaggtaaa
    13741 cattgcgccc cgtatctacg ttataaccaa agtaaatgcc ttccaactcc cctaacatct
    13801 gagtggcgcc aaaaccaagt ccggcgagaa aatcagacgt aacatactgg atataatcat
    13861 tcatgtatcg cttactagaa ggtaagaatt cttcatgtag ccccatcata tcgccaaaag
    13921 gccggactag ttttacattg gtatcatcat aaaaatctag cacttcatca caccggcgct
    13981 ttagttcatc tactgattcg gcacttactc gaaccacata gctcagtttg tacatggatt
    14041 ctttggactg atccaaggta gtttccaatt catctacgga atccaatgca tctaagacat
    14101 tggaatttgt ttcattgtca gattgatagg catggttgtc caagtctttt aattcctttt
    14161 tcttattgcg aacggtagca agtgcctttt tgttcgtcac aatctccaca ttcatactag
    14221 tgtcaattgg gaatgtaaat tgttgttgct ggtaatagaa aagttctgag gagggaaact
    14281 ccatttcccc cacaattgta ttaattgtta agtacgctac atatgattca tgattctcat
    14341 gttccatgca taagtaccgg ggcttttctt caatcaaaca acgactggga cgtaataagt
    14401 cgtaacgttt caccaaagtt tccgacttca gctttttctt tggtagctga aattcatatt
    14461 cttcaaatgg tgtacccttt tcgccataga tatgttcaat caaatacatc agatcactcg
    14521 gtgtgactcg tctgacttta aatcttcgag ccaatttact ttccattagt ttttcaagct
    14581 tggtatatcg gcgaatttct tcattggata aggaaacaaa atcgcccatc aaatgatgat
    14641 tcactccata gacaaattct tgaaagccgg agaagaaaga tttcttgaga ttctttagat
    14701 tgacctcttc atctgtcgca atcagtttga agccaataaa aaagcgataa tcaatctgac
    14761 tatcgccaat catggatact aatgcctctg tctgtaaatc aattcgttgt ttcgctactt
    14821 ctgttaatcg cccggtaatt tccttttttg atcgttcttg cgtggcacga atactacttt
    14881 ctgtggcaat ttgtaaggca tgaatcttgc cttcacgatt ttgcgcaatc aattgacgaa
    14941 agttatcgtg gacttgatat ttctgttcag gtgacaaaaa ggagtaattg taaggtatta
    15001 actcatagta ggcaaaacat tctccctctt gattaaagac aagattattt tcaatatact
    15061 taatcgggta catagttcac actccttacc agagtaatgg gttcgtccca tcgtttcttc
    15121 tgttctttga ctttctttcc tgcgaaagtg accttaaatc gtaagtgata gctaataaaa
    15181 gatcgtaaaa atcccaatgg tttcttaccg tcaaacgttt tttgcgacat gaaccaagta
    15241 actgccaccg gtatacctaa gtattttaaa aaggccccct ctatcataga tagtggaggc
    15301 acatgggcaa atacgatgac aaacagcaag gataaaacaa accaagccat ctggttaaaa
    15361 gtcacaggaa aaggcagttg aaagtcatta attgcataaa tgaccttttc aactgcccaa
    15421 atactggtgt agctttttat tttcttcatc aaatcattcc tttaaaaaga aggatagttt
    15481 agaaatcaac tacccttcaa taattttgtt aatatggata ctcaaaaaca cctcgactcg
    15541 ttaccaaata attgcctcca atctccaagt ctctaccaca cgcatcaaaa tcaatatacg
    15601 tcttcaagtg ttctggtact tctccaaaat caccagcttc tacaatcaag aaatatgcta
    15661 catcactcat atcatcgcaa tctggataat agataatatc atccacatgc tctaccatct
    15721 cctcaaaaga attgaagaaa gcatgttgaa tctcagaagc aacttcacca actggcgttc
    15781 cttcaagttc ctccgctaag ccacacaacc gattaatttc ctcaattggc gtatactcat
    15841 caatctcaaa gggaagttca taatcatgaa ttgcatattc ctcgtattcg tcatttaacc
    15901 caattcgttc tttcacttca tcaaaatcga caggtggggt aaaccacgcc cccaccaatt
    15961 caccttcgtt atatttcccg aggttcgcaa tataaacacg catctgttcc atcgtgcttc
    16021 ccccttacgc accgacaatt cggttaaaca aactgagtaa gacatccttc accccagaag
    16081 cattaaagac caatccgacc gcaatcaaag caatcaccaa aaagccaatc agtttggaaa
    16141 actcccgctt aaatcctaaa aacaaaccaa tgaccacaat tgccagtaag actaaggatt
    16201 gggcattggt taaaaaccat tgatatatgg ataggtaagt tcctgatatt ataaatatct
    16261 caaacttttc cccccattca cacggagcgt gcgactttca ccgcactccg cgttccatcg
    16321 tgtttatatt gaaaagtatt tatgtagtag acctatatca agtttaattc ttgattgcca
    16381 gctaatactc tatatttatt tagtcttatg agctggactt tatttagctt caatagagct
    16441 atatattttt gtattgtttc ttctgtttta gcgtgtatta gacggtgtac cgcctctttt
    16501 accaaaataa gatttttata atcgtcattt ccaccagctc cagttggtac aatatgatga
    16561 caatgtattt cattagcttg ttgaaattct tccagagtta tagcacattt tccatgttga
    16621 gcagaaaata gcgaaattcg attgtctgcg tattctattg tgcgattttc caaagataat
    16681 ttcattaact ggtacatcag taatttatca acacttagat tggtgtggat aaattttctt
    16741 ccctctagag tgtagcaatt cgctaaatac acctttgcca ttggttttct gtgttggata
    16801 tatccaacag ggaaaatagg ttcgccctcg ccagcaacat accgaagcat tttagattta
    16861 ccatatcttg ttgcctcctt tgtcgtaagt tgtctgcctg tttttctcaa tctgcccctt
    16921 ttgtttgtac ctaatctgtt agtgaagata gccattacag ctctattcag tttcatacaa
    16981 tccagattta cattcgttgc aatctgataa taattttgca tacctaatac cattgaatta
    17041 taaagtcttg tttcttctaa ttccgttttc ccttttgagg gagaagcaat tttctttgcc
    17101 tgtgatacca gcttgtcttt ttgttttttg aattgtttat cacttatatg tgatttaaca
    17161 actaacttct ttcttctcgg tatcatttta atcttgaaac caagaaagtc ggagtatctt
    17221 tttctaacat ttacaattct tgttttttct tgtgatactt ccaattttaa tcgttgttca
    17281 atccattgag tgattgctat tttggctttt tcagcacttt ccttgtagcg gcagaaaact
    17341 ctaaaatcat ctgcataacg aaccatatac atttccttta actttgtttt cttcatttct
    17401 ttgtaagcgt tgcttttaca tgggctaccg cttttattga tatgaaccac atagtttttt
    17461 gtaacaggat tttcctgcca ttggctttct acccaatggt ctaactcatt aagaacaatg
    17521 tttgctagta gtggggatat tattccccct tgcggagttc ctttctctgg atatgttatt
    17581 gtaccgttag tcattttgat tggagctttt aatatccttt ttaaaatgaa tatcaagtgc
    17641 ttgtcccgta ttcccatagc ccatatctgt tttatcagct tggaatggtt cacgttgtca
    17701 aagaaaccct taatatcaaa ttcaatcaca taatgtagct ggctgatttg taggcggtta
    17761 taggttgctt ttattgcatt ttcaactgac ctattaggtc taaaaccata gctgttttca
    17821 ctaaatttag cttcgcagat tggttctaat acttgtttta tacattgttg tataagcctg
    17881 tcccacatac aaggaatacc tagaggtctg gttttaccat ttggttttgg tatttctttt
    17941 cgcctcactg gcttaggtcg atacccatgt ggactgcctg caacaatgta tcttactttt
    18001 ttgacaactg tttctgctgg caatttgcca atatctttaa ttattgtacc gtctgttcct
    18061 gctgttttac tgcctgcatt tgctttgata tttcgatatg ccagtaatat attttctctc
    18121 gataggataa tatccataag atgagtgaaa atatctccat tcttactttt ggcatataaa
    18181 tcatcaaagg tgttttgcat ttcgtaatat tcagcgtgtc ttaaatcttc tacacacaat
    18241 gtttcatttt tatttttagg cataaggctt cactccttta cttgaaattg agtgagcttt
    18301 tttatcttac tcgtaatcct tattgaaaca acataatact tttctaatat atacacgact
    18361 tgagggcatt cctccattct cattacaaga atttcttagg ttcgccctct gcttttcagc
    18421 attagttcaa ctgcttattg ttcttcgtca gtttattctc ttgatgagaa cccaatacct
    18481 ttcctcgttc cgataatctt atctatacat atatatcctt aggtcactag ctataagcct
    18541 gctggcttga taccgcctgt aacgttatat ggcatttcat aacctatatt cttactttgc
    18601 cacaccagct acgatttctc gtacctttgc atttctacaa agttcaaatt tagacccgta
    18661 cattcgctgt ttcgtcagtt ccttatagaa cattctaacc atagatattt tatagacccc
    18721 cgacctgttg gatacgctac ccacctctgg atagttttcg atagaagata tatgttcatc
    18781 aactattacg gtatctttca gccgtcttta ccgagcttca aacattctga gtttcctcat
    18841 acaatgcttg tcggaggctc agggcaggcg tttcagagcg ttacttcatc attccacctg
    18901 tcagattatc agttctccta ataaccaaac tttttcgttc ttttattagt gccttagctt
    18961 ttcactaagg aacgagtcgc acataaattt tgtccgaaat tcatcttgtc actttccttt
    19021 cttttccatt gctcgatccg ctgctgcact tgcattcatc aagcacatca cagtaacacc
    19081 aagcatactg ccaccaaata ccaaaaaaat atcttttaca attcccatga cgattcctct
    19141 tttcatataa tcaaattttc aattgccata ctttgttgct ccaaaatctt tttatgtcgg
    19201 tctgataagc gtgcattctg taccatatcc tggatatagg ttgtccgatt caatcgatcc
    19261 aactccttag ccattttcca cgtaggggcg acttgtcggc ctagccaatt caaggttcgt
    19321 tcaatggtat acggttttgg ctcggaagtt aattgaatct ctttccgatt ccgtccaata
    19381 aaacgccccc attgttcatt caagggccag ttggttcgcc ttttaccttc tactttatcc
    19441 gcaaatcgca agtaacgatt gataatggaa aacgtggtgc tctcaatgct acgacctttt
    19501 aacaaatctt gaatcgcatg aaaagcacgg tcatttttca gtcgaatctc aaaacgattt
    19561 ttcgtctcag tttggtctaa tggaatccct aacttgatat attgttcata gtctttttca
    19621 tacaaacaga aataaacctc actcttgaga ctgccgatat ataaggtatt gcccatgccg
    19681 tccttttcat cagcctttaa aagttccccg gaacgataac ttttaaaggt acgaaacaag
    19741 gagatacatt cttccttctg acattttttc gttagctcag gtatatccaa tagccccact
    19801 cgatcattga ttgccaagtc caagcgtttc atcacgccac cggcctttag acaatcctcg
    19861 aaaaagtcgt accagctacg cttttgagcg agtaaaaaag tttcaaattg tcggcaacct
    19921 cgccccttta attctaaaag cacaccttta tcttcttcat gagacaacat caccacaata
    19981 tcacccatga catactgttc ttgataggag taaaaggcat aatcttcatg gagcatatac
    20041 tcaaatttca aatggagaat ttcttgaata atttttcgtg catccgttgt gggaaagcga
    20101 atccttacat agtcaaacag taacgtcaaa ggcaaatcgg gattaaagct ttccagttgg
    20161 tcaaaaattt tcctcatggt ttttatagat gcggttacgc caccattttc tatctgattt
    20221 aagtacggcc gactaagtcc acaagccgta gccaatttat tttgtgaaac gccgtattcg
    20281 ttgcgtcggt cttttaatag ccgataatct aaattccgtt gtgccaagtt ctcacctcca
    20341 aattcaaggt gttaactttt tcaaatgagt taacacctat acaaattact tactatcttt
    20401 gatacaacaa gcattcttaa tggttgctta cttatttttt gtgtttttgt acccccctgt
    20461 tagatacggg ggtgagacat catggtcggc taacgccgtc catgaacagg cacagcacca
    20521 cggaggattt ctgcttcgcg aatcctccgc ggtcagcgcc taacagtcag ttgtgttggt
    20581 ttaggttact catctgtaat accatttact tccaactctg ctttcatttc taacaagtat
    20641 tgacgaatat ctggttcttt tgcttggtcc gcaatccgct caaattctgc aattacttga
    20701 tttatttcaa ttcgagcatc cgtctcctca ttcattaaat atggatcgcc agcgactttg
    20761 acaccattct tgcgaatatc aaattttaac cgtcgcaatt gttcttctaa ttctgggtct
    20821 gactgaatct ttccaatcac ccaataaatt tctccaatca actcatcata aaccatgtac
    20881 atccaactct tttaatcttc tggcaatatc aaaaatgacc ggaactgtta cagagtttcc
    20941 ggcttgctta tacaactgac tatctgaatt gacttgagaa tctcgatcaa aagcccaatc
    21001 gggaaagccc tgaagtcgcc aacactctct aggtgttagc ttccgaattt gatactgatc
    21061 cgtgacgatc gcttgttggt ctcctgttag taaggtatga acactctgtt ttcccagacg
    21121 acctcggcga cttgtggaat taggaaaagc cagattcaca ctgtcttctg gcttcacctc
    21181 ttgatattct gtttcatttt ttcgaatcat gatttttgta ccaccttgtt tcgttgacaa
    21241 ggtgggggcg gttccagtaa cttcataaac tcgattacct tgttcaaact ttcccggtaa
    21301 tagtcctgct acagagattc catcccttgc tggcagtgcg attttgattc cttcatcacc
    21361 tgttaaggtt ggcgctacgc catctgaacc atagacttgt ccacccattc ctcgattgga
    21421 aggattgata tttaccaaat ttctgatgtt ttgatcagat gttgaagacg gtccaatgat
    21481 aggaaatact tgttcggtac atgcgtctcg agaatgtccg ataaggaata tccgctcccg
    21541 gctttgggga atgtaggcct ttgagttaag cacctaccac tccacgtcat accccaatcc
    21601 atccagcgtt cggaggatcg tctcgaacgt agcccctcct tcgtgattga gcaatcctcg
    21661 gacgttctca aggaataaaa gcttaggtcg gagaagagtg gtgaaccgag cgatttcaaa
    21721 gaataaagta cctcgagtat cggaaaatct ttgccgcttt cccgcaattg aaaaagcttg
    21781 gcacggaaat ccgccgcaaa ggatgtccac tggtccgagg gattgaataa actcatctga
    21841 tacacttgtg atgtcatgca tttccacctc cttacttgta tcatgaattg ctttataact
    21901 acgtctagca aatttatcaa tttcacaaaa accgatacaa tgatggctcg cctgttccat
    21961 acctaaacga aagccaccaa taccggcgaa taaatctaaa aatctcatac attacctcat
    22021 tcattgcaaa gaaaaaagcc aagaacattt tgctcttggc tcatacatta ttaatttgtc
    22081 ctactatttt ttatttaatc caactaattt ccgttgtttt tcattggcct gatcggctac
    22141 atatttaatc accgttttga actcttttgc agtagcattt tccgataaat ccgcaatgct
    22201 cttattgtat ttctttacaa ttttcgttac ttgttctttc tcagtcatgt aattgaacct
    22261 ccaaatcttt cctactatac cctagtggta aaaataattc tagattatcc gtagcataat
    22321 gaacttcgga aatatta
//
LOCUS       DAZKLD010000036        22034 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.036, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000036 DAZKLD010000000
VERSION     DAZKLD010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22034)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22034)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22034
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.036"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(53..1426)
                     /locus_tag="ACG8KR_001117"
     CDS             complement(53..1426)
                     /locus_tag="ACG8KR_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFQ0200501.1"
                     /translation="METTMNPEQMVLSEQIHMLIEQLGGKDNIDSVLPCATRLRIKLK
                     KQPVANAEKLKQQIPSIKYFILLDRYAEIIMDQGLAPAYKELTRQIDHSAPKEKLNPI
                     EKLFGLISEIFTPIIPLFAGSGILKGLVVLATTIGWLSETSGTYHILSAASNAVFYFL
                     PIFLAFSAAKTFKVNGYIAAVIAAALIEPNLTSLLSDSGKAATDFLGIPVVLMQYTST
                     VMPAILGIFFYSFVEKFLKKYIRENMQLVFVPLLSLVITVPLTLMVFGPAGVYLGEGL
                     AAAITWMMDINGPISGAIIAGGWNILVIFGIQWAVNPVMISNISAYGFDRIVPLTGAA
                     NFGMAGAALGVFLRSKRSKTRSISGSAFASILLAGVTEPTVYGIAIPLKKPFVAACIG
                     AAAGGAVMGFAQVKAIAFVFGSLTTLPAFISGTFFWYLAGLAVSLVVAMITTLVSGFD
                     EDLMSYE"
     gene            complement(1426..1821)
                     /gene="rbsD"
                     /locus_tag="ACG8KR_001118"
     CDS             complement(1426..1821)
                     /gene="rbsD"
                     /locus_tag="ACG8KR_001118"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HFQ0200502.1"
                     /translation="MKKGKVINSDISRVIAQMGHFDKLSIGDAGMPVPFGTEKIDLAV
                     SNGLPSFLSVLENVLEELEVQSIELAEEIKTMNPEILEKIKLLLPDTPISFIPHEEMK
                     KELNNCHAFIRTGEMTPYANIILVSGVVF"
     gene            complement(1849..2766)
                     /gene="rbsK"
                     /locus_tag="ACG8KR_001119"
     CDS             complement(1849..2766)
                     /gene="rbsK"
                     /locus_tag="ACG8KR_001119"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006014 - D-ribose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HFQ0200503.1"
                     /translation="MNTVTVIGSINMDTTLRLTNMPKPGETMHAHEIFHAGGGKGANQ
                     AVAAKRSGARTSFIGGVGADSEGQQLLDLLTKENIDTSGIAEIQGATTGQAMIMVDAA
                     GENSILIHAGANNAFHEQEVLKNKQLITNSDFIIAQFESSLDATILAFSIAKDAGKTT
                     ILNPAPARETIPTELLEKTDIIIPNETETEIITGIRVTDHNSLVAAAEKLHELGIGTV
                     IITLGSAGAFYHTEKEHGIVPAFKVDAVDTTAAGDTFIGALSSTLQPDLSNLKEAILY
                     GNLASSVAVQSYGAQPSIPYREALEQSAV"
     gene            complement(2874..3887)
                     /locus_tag="ACG8KR_001120"
     CDS             complement(2874..3887)
                     /locus_tag="ACG8KR_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HFQ0200504.1"
                     /translation="MVKKKITIKEVAKLSGVSITTVSLILNGNTEKFSPKTVQKVLAA
                     KEELGYQPDYFARQMITKETKTIGVLVPDITNPFFSTLMRGIEDILYKQNFVTILCNA
                     DSDHQKEIEYLAELTRRGVDGFIIATSAVSTDAINENLKKQGRPFIVLDQKKSEGFSD
                     AVRTDDFRGGYLAGMHLLSLGHQTIALVYPENPPENVHARIEGFKSALDVYQIPHDQL
                     ILLPTQFSKQGGYQITAELLDSAATGVFALNDELAFGLYRGLEEAGKSIPEDYSIIGY
                     DNIDMCEYIKPKLTTIAQPIFELGQTSAKLLLDRIQFPEKEWEEKRLPVRLEKRFSTA
                     PLK"
     gene            complement(3968..4567)
                     /locus_tag="ACG8KR_001121"
     CDS             complement(3968..4567)
                     /locus_tag="ACG8KR_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HFQ0200505.1"
                     /translation="MNDLKENMIFDLSKTAFVAIDMQKEIVNNGTLSPHTAASILTNN
                     DLLVRTLKRTAALKVLVNVDISTFPYLSQQTDMGGMTASVPPEFTDLLLKDSLKDTDN
                     MLTITKYNPSAFFGTSLDLQLRRRGIETIILSGVATTNGVYATALDAFQHGYHIVLAE
                     DACSDRDKESHQLFIKKIFPKTARVRSTKQIIEAIQQSK"
     gene            4753..5454
                     /locus_tag="ACG8KR_001122"
     CDS             4753..5454
                     /locus_tag="ACG8KR_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0200506.1"
                     /translation="MKYVEVKNEYKRYKMGETTITANDDISFSIEKGELVIILGPSGA
                     GKSTILNILGGMDTPDEGQIIIDDTDIAQFSDKQLTAYRRTDVGFVFQFYNLVPNLTA
                     KENVELATEVSPDALDPVEVLRQVGLAHRLNNFPSQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDFETGKQVLKLLQNASRQHGNTVLIITHNSALAPIADRVIHINDAKVRS
                     VELNDHPSSIDEIVW"
     gene            5464..8175
                     /locus_tag="ACG8KR_001123"
     CDS             5464..8175
                     /locus_tag="ACG8KR_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HFQ0200507.1"
                     /translation="MKTRTYLKAGFREIRQSKGRFIAIILIVMLGTLLFVGVKTAGPV
                     MQKTMDHYVGTAGLSDLQIVSTGGLTQKDIAQAEKIPDAKVETGKQIYYSNTGKNEVI
                     QIFSYNKNNKQNKLQLTDGKLPEKPNQLVLDEKAEDEGYKIGDIYQIDSDELKEKEYT
                     ITGFVRSPLFINNLERGYANVGNGSVDYFVYLPESVFKTDIYSVLYLDFTNVKNLNTY
                     SKAYKDKMEQNQEKAEKYLQDRPEERLEELKKSANEELEPAQQKIADGKAQTTQARTR
                     LEDAKKQLAQQSATIQQLPEAQRTIAVQTLSQQEAQLAEQERQLVEKERELASAEEEL
                     NRNAEKIENLTKPSYLYNERSENVGFQEFGDLSDRIAAIANVFPVFFFFIAALITFTT
                     MTRMVEENRREIGTLKALGYTKVEIAKKYAIYASLASGIGIVLGTILGTNLLPRIIFE
                     LSNERYDIGSALIFYDWPPILQAAAAFFIAAFGAAMIVLFKDLRERPAALLQPKAPKP
                     GKRIFLEYITPLWSRLSFNQKISYRNLFRYKSRMLMAIIGIAGCAGLMVAGVGLKDSL
                     SSVSAKQFGPIIDYQAIVTLDSSTEDSRQKAADMLQKQEKVNEILAIHTQTVELRKKG
                     HASQSLTVIVPSKTADLDRFIHLTDLEENPRSLPKTGIAITQKVAELFDLSTGDHISL
                     YDEDQKEWKVEVKEIVKNYLGSFIYMSPEYYASVTDQSMMDNAFLLRTDKMTTKEEEM
                     LSEKLLATDAVTNTSFMSKQIETQEESMANLDSVVLIFVVLSGLLAFIVLYNLTNINI
                     SERVRELSTIKVLGFFDKEVTMYIVRENIIFTLLGIIGGFGVGYILTDFILQQASMEN
                     VIFPLVITWWAYVLSAGLTIVFTVIVMIVTHFKLKHIDMIDALKSNE"
     gene            8626..10254
                     /gene="treC"
                     /locus_tag="ACG8KR_001124"
     CDS             8626..10254
                     /gene="treC"
                     /locus_tag="ACG8KR_001124"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008788 - alpha,alpha-phosphotrehalase
                     activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HFQ0200508.1"
                     /translation="MTFKDKVIYQIYPKSYKDSNGDGIGDLKGIKEKLPYLNDLGIDM
                     IWLNPIYPSPQKDNGYDISDYTAIDPIFGTMEEFEQLIAEAKKYRIEIMLDMVLNHVS
                     TEHEWFKKALAGDQHYQDFFILREEPTDWVSKFGGSAWSPFGDTGKYYLHLYDRTQAD
                     LNWRNQAVREELFKVVRFWMEKGVKGFRFDVINVIGKDEQLKNNKENDGKPEYTDKPI
                     THTYLKELNQATFGTDSTIITVGEMSSTTIENCILYTEPSREELSMVFNFHHLKVDYE
                     NGNKWSTPPFDFAELKRLFHTWGEKMSEGNGWNALFFNNHDQPRALNRFVAIDQYRKQ
                     GAEMLATMIHLNRGTPYIYMGEEIGMIDPDYSSISDYIDVESLNAYKLLLEQGFTEKE
                     AFRRIKAKSRDNSRTPMQWSDTEQAGFTTGKPWLKVGGKLEEINVEKELSSEDSILSY
                     YKKLIRLRKTYPIVAQGDYHAYEANHPEVYGFLRQFEGQQLLVLTNFYDKDTRIAIPE
                     GFTEANILIDNYSDTVLHQSLELKPYQAIALLKN"
     gene            complement(10314..11495)
                     /locus_tag="ACG8KR_001125"
     CDS             complement(10314..11495)
                     /locus_tag="ACG8KR_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFQ0200509.1"
                     /translation="MKKETDTTNRLIRFLGGKTSYYVLGIVILLALIIYFFHQISFIF
                     KPVGIIIATILPPLLFALILYYLLEPVIKRLSKHLSRNLSVIIVYVIILFLLGLGSIW
                     LIPFLEEQAKTLISSLPDLFQDFQESLRQFLEKTPFADSFNQFFASIDDVTSDIAGFI
                     GNYWESGAQRISNIFSTVTAIFITLFTGPIVAFFLLRDPQKFYQSVLAIVPPAFRTDF
                     KNLTKIANQQLGSFLKGQIIASFILGAVYWVCFLLIGLEFASVLAISAGLLCIIPYIG
                     PFIAFFPGLIIAFQDSTFMAVKFVIVWFAVQLLHGDLVIPRVMGDRLQIHPITILIVL
                     LVMGDLMGIVGVIFGIPIYTLVKLLVTFIFRKFKQRYNKFYGDKGEYENSSFSEEDYF
                     K"
     gene            11747..13270
                     /locus_tag="ACG8KR_001126"
     CDS             11747..13270
                     /locus_tag="ACG8KR_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HFQ0200510.1"
                     /translation="MKRLIGILAMLVLAGMLTACGASGKAEDSKEKLSVMTTFYPMYD
                     FTKAIVGDEGEVELLIPAGTDSHDYEPSAKDMAKIQDTDIFVYNDENMETWVPAIQKT
                     LQEGNVHTIKATEGMLLLPGSEEGHDHDHEHGEEGHTHELDPHVWLAPSLAIKQVANI
                     RDQLIEAYPEKQEVWTKNAAAYTEKLQALHQLYQETFKQAKQRSFVTQHAAFNYLALE
                     YGLNQVSIAGLSSSEEPSAARIAELKHFVKEHGINYIYFEENAKDSIARTLANEAGVS
                     LEVLNPLEGLTNEQIENGENYLSIMEANLEALKKTTETENPLEDTLTPKKEKSVYNGY
                     FEDEDVQDRSLSDWAGTWQTVDTYVEDGTFDPVFEYKEKLNQDKTAQEYKEYYTKGYQ
                     TDIASIKISEKEMAFTFKNGTTKRSNYRYVGKEILTYEAGNRGVRYLFEAEDKESGAF
                     RYIQFSDHTIAPADSEHFHLYAGNESQEVLLKEMENWPTFYPDTMDGREIAQEMLLH"
     gene            complement(13339..14067)
                     /locus_tag="ACG8KR_001127"
     CDS             complement(13339..14067)
                     /locus_tag="ACG8KR_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HFQ0200511.1"
                     /translation="MIPIYICEDDPKQLEVLSTVIKNRIMIENLDSYVHISTSDPEEL
                     LNAAKIRTSSFGIYFLDIELEDSEMDGVAIGKLIRELDPLGKIIYVTSHTEMSMKILK
                     SNIEPTDYIVKEDLYDLKENVEHILSKIFEDFQMSQIQKDIFKIEFNDEIKFLPIKDI
                     QYFSTSLGAPHKLEVHLTHAQLQFYEKIKEIEKMHRNFIRCHKSYVINTQNVRSINKK
                     TREVTLANGETIPASIRGLKKLIS"
     gene            complement(14080..15360)
                     /locus_tag="ACG8KR_001128"
     CDS             complement(14080..15360)
                     /locus_tag="ACG8KR_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HFQ0200512.1"
                     /translation="MFLWSIALQYLIYTICILLIDHHIRYKYLFAGVILISCILIGWV
                     YSILGIFTALIAIGMLMLTFFFFSTSRQQLVYALPFGLLTSLLGDHLTSIGDFFVLKS
                     SVIEPGDSLQYFHIVLSAFLSALLAFCLKKSLETWFHTVDRRMIGTIGTLILFTYYII
                     IFYTRFSGETPDVLALNTSFFILYLCVGLIILVYYWKISNKKLADQLLEQQVRMQQDY
                     IKDLEKNYQDLREFKHDYQNLLFSLHSYISEGDLEGLRSYYDQTILPTRQMMNLFPAN
                     LSMLDNMKVPEIRSLLSLKLMMAQEKGLIVQLHFPEPIELDTKHTVNLVRMLGIILDN
                     AIEGASAAKKKKIELTILKKNHSVMIRVINTTTNTLPLNQLKQKGFSTKTNPKNEGLG
                     LFILDELTKTNPYLFLETSIENQRFSQTIYIQIK"
     gene            complement(15501..16103)
                     /locus_tag="ACG8KR_001129"
     CDS             complement(15501..16103)
                     /locus_tag="ACG8KR_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HFQ0200513.1"
                     /translation="MKDKVVNKLLEALFSGDKSEEDMVYVQVKFVLEVLLNNLGKLAV
                     VLVFSLVTGSWAETGITFLSYICIRRYAYGLHSDSEFVCLLWTLLYLWGVPLVMKHLQ
                     LTISFPMMVFLLISCFLLLLRYGSRGTAINPIEPEKRPPLLKKAISMFLIFSLITLFF
                     SASYFSTYLLLGIVLEIATLLPITNYLMNFGGIFHEKNNH"
     gene            16348..16554
                     /locus_tag="ACG8KR_001130"
     CDS             16348..16554
                     /locus_tag="ACG8KR_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0200514.1"
                     /translation="MENEQLFEVGETYAFKSDLFESPIKGKIEKIYENSVMIRVMKCA
                     VEDNDTAHNLHWQLVVRKSSTIEE"
     gene            16866..17585
                     /locus_tag="ACG8KR_001131"
     CDS             16866..17585
                     /locus_tag="ACG8KR_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HFQ0200515.1"
                     /translation="MPIIVIIVAGTIGAGKSTLTEMLAQDLETKPFYENVEDNEVLPL
                     FYSNPEKYTFLLQIFFLNKRFLAIKDAFSHDDNVLDRSIYEDSMLFHLNADLGRVSEV
                     EVKQYEGLLETMLKELEEISPQKKPDLLVYIRVSFETMLARIKKRGREYEQLEQDPEL
                     YSYYKELNRRYEEWYEQFDICPKIVIDGDKYDFVADPACGEQIVQEIKMRAKKMTEEA
                     DAQSIRYTTNNPKAKGIFTTK"
     gene            complement(17666..18349)
                     /locus_tag="ACG8KR_001132"
     CDS             complement(17666..18349)
                     /locus_tag="ACG8KR_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family protein"
                     /protein_id="HFQ0200516.1"
                     /translation="MQTSFHEGRTIGLLLTFIGGAMDSYTYIQYGAFASAQTGNIILS
                     IIQAFDGEWLSVGKKVLSTLFFFLGILLTKYLIDYFRKKEKHFWRLFVLYYEAVIFFF
                     VSLTPVHEHPALVTILIAFTASIQWVAFDKINGRAYTNLFTTGNLKGVATNLYDYCIS
                     KEKADFDRFFHYLSVVLAFIAGAIVLVFCHHLFGAHSILIVSILFLIMALTQTFHLWQ
                     FYHTNRFTI"
     gene            18566..20752
                     /locus_tag="ACG8KR_001133"
     CDS             18566..20752
                     /locus_tag="ACG8KR_001133"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HFQ0200517.1"
                     /translation="MNEKEILRLQKELKRWENKYKKLQQVFRLQEEIIATYEAAYGEA
                     PGLIEEEVQVKESEGNSSFFKMMEKRSYNRFNQEQKAAITYDMKKHLRIIAGAGSGKT
                     QTICAKAVYLMTQKQVDEERILMITFTRNAANELKKRVDNFSQRKTAVHIGTFHSIFF
                     RIYNEICRKFPEVAMQGIQGDFSKDTAQKVNAVLQQLIRKYNLYIFDQYGEKTIASRL
                     DYWQNMNLSVEEMVQLVKEKYDSIDKNARQPISERLYGLLTELQEQKRSQQLLEFNDV
                     LQNLKSALENEEIQHYIGQKYDYLFIDEFQDTNPLQWEIVKRMTKENRIKLIVVGDDD
                     QSIYGFRGSEPAYIKNFEKEYPTKTLFLLTNYRSRAAIVQGANRLISYNKNDRIPKAM
                     IPAQKEAGVMEAYLFSDTKAESQWLIKQIQTFIQKNGKYKESIILYRSASQTQQLVQS
                     LLKTNIPFVLEADSPYEGIFGIKSFQHFYQKIVHWQTALNLQKKNQAYQQLLRQLMAD
                     CYLKKSECDQYFSPKYQNIAVADYILSISPNLKSKSAEIREFEEALRKTDQHQEIYSL
                     VQSYLHLPRIAKEIDQSDQEWIREEVKQHATFRRLQALSQETQATAKELKTRLIAYRQ
                     GKLDALCIQSIHKSKGLSYRNVFLIGCNEGSLPYNGATEKKVIDFNEIKAEPVTTIEE
                     ERRLFYVAMTRARNRLYLCVPQMKNSRKLKVSRFIKETGSRVKKGK"
     gene            21098..21508
                     /locus_tag="ACG8KR_001134"
     CDS             21098..21508
                     /locus_tag="ACG8KR_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFQ0200518.1"
                     /translation="MIKVYTSPSCTSCRKARAWLQANQLEFEEKNIFAEPLTESEIKK
                     ILAATEGGVQDIISTRSKIYEKLGIDFNELTLKQMIVLFKEYPSLLRRPILIDEKRIL
                     IGFNEDEIRSFVPREIRCVARNLIYLQNNNILNS"
     gene            21522..21878
                     /locus_tag="ACG8KR_001135"
     CDS             21522..21878
                     /locus_tag="ACG8KR_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFQ0200519.1"
                     /translation="MEVKVYGSAHCVTSLKAKQWLEHHQLPYQFVDLEERDLSTEEAE
                     ELCSFEEVQAEQLFATWSEAFHNIAIDLSCVGKKQLIFLCTQHTNLLRRPLIIIDEVL
                     FIGYNEQLMEQLILNK"
BASE COUNT         7529 a         3914 c         4284 g         6307 t
ORIGIN      
        1 aaaaagactg aaatatgtga taaacatctc atacttcagt ctttttcatt aactattcat
       61 aagacattag gtcttcatca aacccgctaa cgagagtagt gatcattgct acgactagac
      121 taacagccag tcctgctaag taccagaaaa aagtaccgga aatgaatgca ggcaatgtgg
      181 tcagactacc aaatacaaaa gcgatcgctt tcacttgggc aaatcccatt acagctccac
      241 cagcagctgc accaatgcaa gcagcaacaa atggtttttt caatgggatc gcaattccgt
      301 aaacagttgg ttcggtcact cccgcaagta aaatcgaagc aaaagcagaa ccggagatcg
      361 aacgggtttt tgaacgttta cttcgcagaa atacacctaa agctgcacca gccattccaa
      421 aattggctgc tcctgtcaac ggaacgattc ggtcgaaccc ataagcagag atattactga
      481 tcatgacagg gtttacagcc cattggatac caaatattac caaaatattc caaccaccag
      541 cgatgattgc gccactgatc ggtccgttaa tatccatcat ccaagtaatt gcggcagcta
      601 aaccttcgcc aaggtaaaca cccgcaggcc cgaataccat taacgtcaaa ggaaccgtga
      661 taactaatga aagtaaaggt acaaatacta attgcatatt ttcgcgaata tattttttga
      721 ggaatttttc aacaaaagaa taaaagaaaa tacctaaaat cgccggcatg acagtagacg
      781 tatactgcat cagtactaca ggaatcccca gaaaatcagt agctgctttg ccactgtctg
      841 aaagtaaaga ggtcagattc ggttcaatca gtgctgcagc aatgacagcc gcaatatatc
      901 cattcacttt aaacgttttg gcagcagaaa aagcaagaaa gattggcaga aaataaaaga
      961 cggcattcga cgcagcagac aaaatatgat aagttccgct tgtttcacta agccagccaa
     1021 tcgtagtcgc taaaacaacc agacctttta atataccaga accggcaaac agcggaatga
     1081 tcggcgtaaa gatttccgat atcaaaccaa atagtttttc gatcggattt agcttttctt
     1141 tcggagctga atgatcgatt tgacgcgtca attccttgta agcaggagcc aatccctgat
     1201 ccatgattat ttctgcatat ctatcgagta aaatgaaata cttaatggaa gggatttgct
     1261 gttttaattt ttctgcattg gctactggct gttttttcaa tttgatacga agccgagtcg
     1321 cacatggaag gacagaatca atattgtctt ttcctcccaa ctgttcaatc agcatatgaa
     1381 tctgctcgga cagtaccatc tgttcaggat tcattgtcgt ttccattaga agaccactcc
     1441 actcactaat ataatattgg cgtaaggtgt catttcgcct gtacgaataa atgcatgaca
     1501 gttattcaac tcttttttca tttcttcatg tgggataaag ctaatcggtg tatctggtaa
     1561 caataatttg attttttcca atatttccgg gttcattgtc tttatttctt ccgccaattc
     1621 aatcgattgt acctcaagtt cttctaaaac gttttctaaa acagataaaa aacttggaag
     1681 accgtttgag acagccaaat ctattttttc agtaccaaag gggacaggca tgcctgcatc
     1741 cccgatactt aatttatcaa agtgccccat ttgtgcgatt actcgtgaaa tatctgaatt
     1801 gattactttt ccttttttca tgtttgtttc ttcctctcat tgttaatttt agactgctga
     1861 ttgttctaaa gcttcgcggt aagggataga aggttgcgca ccataagact gcacagcaac
     1921 agaagaggct agatttccat aaaggatcgc ttctttcaag ttactaaggt ctggctgaag
     1981 tgtgctgctt aatgcgccaa taaatgtatc acctgcggct gttgtgtcaa cggcatccac
     2041 tttaaaagca gggacgatac cgtgttcttt ttctgtatga tagaaagcgc cggcactgcc
     2101 tagtgtaatg attactgttc caatacctaa ttcatggagt ttttcggctg ctgcgaccaa
     2161 actgttatga tctgtcactc tgattcctgt aatgatttct gtttctgttt catttggtat
     2221 aatgatatct gtcttttcaa gtagttcagt aggaatcgtt tcacgagctg gtgcaggatt
     2281 caggatcgtt gtttttcctg cgtcttttgc aatagaaaaa gctaggatcg tcgcatctaa
     2341 gctactttca aactgagcaa taataaagtc actgtttgta atcagttgct tgttttttag
     2401 gacttcttgt tcatggaaag cattatttgc ccctgcatgg atcaagatac tattttctcc
     2461 tgctgcgtct accataatca ttgcttgtcc ggttgtggcg ccttggattt cagctatccc
     2521 agatgtatcg atgttttctt ttgtcagtag atctaataac tgttgtcctt cgctgtcagc
     2581 cccaacgcct ccaataaaag atgtccgtgc gcctgaacgt ttggctgcca ctgcttggtt
     2641 ggcaccttta cctccgccag cgtgaaaaat ctcatgcgca tgcatcgttt cgcctggctt
     2701 gggcatgttg gttaaacgaa gggtagtgtc catattaata cttccaatta ctgtaaccgt
     2761 gttcatttta caaacctcca actatctcat ttattttgat gggaaaatta ttttttaaaa
     2821 cgttttagta aaacgtttta atagttcgat aaaaatatag cacaccttga aagctatttc
     2881 aagggtgcag ttgaaaatct tttttctaat cgtacaggta atcttttttc ttcccattct
     2941 ttttctggga attggatccg atctaacaat aacttggcag atgtttgtcc caattcgaag
     3001 attggttgtg cgatcgttgt cagtttcggc ttgatatact cgcacatatc tatattatca
     3061 tagccaataa tgctgtagtc ttcagggatg ctttttccag cttcctctaa tccgcgatat
     3121 aatccaaaag ctaattcatc atttaaagca aacacaccag ttgcagcaga atcaagaagt
     3181 tctgctgtga tctggtagcc accttgtttt gagaactgtg taggtaagag gatcagttgg
     3241 tcgtgaggga tttggtaaac atcaagagca gatttaaacc cctcaatccg agcgtggaca
     3301 ttttccggtg gattttcagg atagacaagg gcgatcgttt gatgtcctag actaagcaag
     3361 tgcatgcccg ctaggtaacc accgcgaaaa tcgtctgttc gtacggcatc actaaatcct
     3421 tcagattttt tctgatccaa aacaatgaag ggccggcctt gttttttcaa attttcgttg
     3481 attgcatctg tagagacagc agaagtcgcg ataatgaatc catccacacc tcgacgtgtg
     3541 agctcggcta gatattcgat ttctttttga tgatcagaat cagcattaca taatatcgtg
     3601 acgaaattct gcttatacaa gatatcctca attcctcgca tcagtgtact gaagaatgga
     3661 ttcgtgatat caggaacgag tacaccaatt gttttcgttt ccttggtaat catttgtcta
     3721 gcaaaatagt caggctgata gcctaattct tctttggcag ctaaaacttt ctgaacagtt
     3781 ttcggactga atttctccgt atttccattt aatatcaagg agacagttgt gattgaaacg
     3841 ccggaaagtt tagcgacttc tttgattgtg atcttttttt tcaccatgtt catactccta
     3901 tctaaatcct gtggttattt tcccatgaaa tcaaaaagaa gtaaatgaaa atcacgtgtg
     3961 ccagtactta tttgctttgt tggatagctt cgattatttg tttcgttgag cgtactcgtg
     4021 cagttttcgg aaatatcttt tttatgaaca attgatggga ttctttgtca cgatcactgc
     4081 aagcatcttc agcaagcacg atatggtacc cgtgttgaaa ggcatctaat gcagtagcat
     4141 atacaccgtt tgttgtcgct acaccgctta aaatgatagt ttcgattcca cgccggcgaa
     4201 gttgaagatc aagagaagta ccaaaaaaag cacttggatt gtattttgtg atagtcaaca
     4261 tattatctgt atctttcaaa gaatccttca aaagtaaatc agtgaattct ggaggtacag
     4321 aagcagtcat tccacccata tcagtttgtt gactcaaata agggaaagtg gagatgtcta
     4381 cgttcactaa aactttcaag gcagcggtac gtttcaatgt tcttactaac aaatcattgt
     4441 ttgtcagaat agatgctgca gtgtgtggag agagtgtacc gttatttacg atctcttttt
     4501 gcatgtcgat cgcgacaaaa gcagtttttg ataaatcaaa aatcatattt tcctttaaat
     4561 cattcatgat gttccttctt tcaaataaca atgtttttta tgtaagtaaa tcataaatcg
     4621 tttatttgtc aactaaatta aaggattcct ttctttagtc tagagaaatt cagtggtaaa
     4681 ataaataagt gtgagaaaac gcattcgatg catagatata gcgagaaata tcgtgaaaat
     4741 ggaggaatca gagtgaagta tgtcgaagta aaaaatgaat acaaacgata caaaatggga
     4801 gaaacgacca ttacagcaaa tgacgatatt tctttttcaa ttgaaaaagg cgaattagtg
     4861 attatacttg gtccaagtgg agcaggaaag tcaacgattc ttaatatttt aggtgggatg
     4921 gatacacctg atgaaggtca gatcattatt gatgacacag atattgcgca attttcagat
     4981 aaacagctta ctgcttaccg acgaacagat gtagggttcg ttttccaatt ctacaatctc
     5041 gttcctaatc taacagcaaa agaaaatgtg gaattggcaa cggaagtttc tcctgatgcg
     5101 cttgatcctg tagaggtatt gaggcaagtg ggcctagctc atcgattgaa taactttccg
     5161 tcccaattat caggaggcga acagcagagg gtttcaattg ctcgtgcact agctaaaaat
     5221 cccaaacttc ttttatgtga tgaaccgaca ggcgcgctgg attttgaaac gggcaagcaa
     5281 gtattgaaat tattgcaaaa cgctagcaga caacacggca ataccgtttt gatcattaca
     5341 cataattctg ctttagcgcc catcgctgat agagtcattc acattaatga tgctaaagta
     5401 aggtctgttg aattaaatga tcatccttca tccattgatg agattgtatg gtagggggat
     5461 tttatgaaaa caagaacata tcttaaagca ggttttcgcg aaattcggca atctaaagga
     5521 cgattcattg caatcatcct tattgtcatg ctaggaacac ttttatttgt aggtgtcaaa
     5581 actgctggac ctgtcatgca aaaaacgatg gatcactatg ttggtacagc aggactttct
     5641 gatttgcaaa tcgtttctac tggtggcttg acacaaaaag atattgctca agcagaaaag
     5701 atcccagatg caaaagtaga aacaggaaaa cagatctatt attccaatac tggcaaaaac
     5761 gaagtgattc aaatattttc ttataacaaa aataacaagc agaacaagct acaattaact
     5821 gatggaaaac tcccggaaaa accaaaccag cttgtattgg atgaaaaagc ggaagatgaa
     5881 gggtataaaa tcggtgatat ttatcagatt gattcagatg aattgaaaga aaaagagtat
     5941 accatcacgg gctttgtccg ttctccattg ttcatcaaca atttggagag aggatacgcg
     6001 aacgtcggaa atggtagcgt cgattatttt gtctatttac ctgaatccgt ttttaaaaca
     6061 gatatttatt ctgtcttata tcttgatttt acaaacgtaa agaatttgaa tacgtacagc
     6121 aaagcttaca aagataaaat ggagcaaaat caagaaaagg cagaaaagta tttacaagat
     6181 cgacccgaag aacgcttaga agagctgaaa aaatcggcca acgaagaatt agaaccggca
     6241 cagcaaaaaa tagcggatgg caaagcacaa acaacgcaag caagaacacg gttagaagat
     6301 gctaaaaaac agttagcgca acagtcagca actatccagc agcttcctga agcgcaacga
     6361 acaatcgctg ttcaaacatt atctcaacaa gaagctcaac ttgctgaaca agaaaggcaa
     6421 ttagttgaga aagaacgtga actggcctct gcagaggaag aattgaatcg aaatgcagaa
     6481 aaaattgaaa atttaactaa accatcatac ctatacaatg aacgttcaga aaatgtcggt
     6541 tttcaagaat ttggtgatct gtctgatcgt atcgctgcga ttgccaatgt ttttcctgtt
     6601 ttctttttct ttattgccgc attgatcacc tttacgacaa tgacacgtat ggtagaagaa
     6661 aaccgcaggg aaatcgggac attaaaggcg ttaggttata cgaaagttga aatcgcaaaa
     6721 aaatatgcaa tctatgcaag tctagcctca gggataggta ttgttttagg aacgatatta
     6781 ggaacgaatc ttttgccaag gatcattttt gagctgtcta acgagcggta tgatattggg
     6841 agtgcgctta ttttttatga ctggccgccg attttacaag cagctgccgc attcttcatt
     6901 gccgcatttg gcgctgcaat gatcgtttta ttcaaagatt taagagaacg gcctgctgca
     6961 ttgcttcaac cgaaagcacc gaagccaggg aaacgcattt ttcttgaata catcactccg
     7021 ctgtggtcac gccttagctt taatcaaaaa atcagttacc gaaatctctt ccgatataag
     7081 tcaagaatgc ttatggcgat tatcggaatt gctggatgtg ccgggctgat ggttgcaggc
     7141 gtgggattga aagattctct aagttctgtt tcagccaaac aatttggacc gatcattgac
     7201 tatcaagcaa tcgttacatt ggattcatcc acggaagata gtcgtcaaaa agcagcggat
     7261 atgctgcaga agcaagaaaa agtaaatgag atcctagcta ttcatactca aaccgtagaa
     7321 ttgagaaaaa aaggacatgc ttcgcaaagt ctgacagtga tcgtaccaag taaaacagct
     7381 gatttggatc gttttatcca tttaactgat cttgaagaga acccacgctc cttgcctaaa
     7441 acaggaatcg ctattacgca aaaagtagca gaactatttg acttatctac aggagatcat
     7501 atttcgcttt atgatgaaga tcaaaaagaa tggaaagtcg aagtaaaaga aatcgtgaaa
     7561 aactatctag gtagctttat ttatatgtcc cctgaatatt atgcttcagt tactgatcaa
     7621 tcgatgatgg ataatgcatt tttgctgcgt acagataaga tgacaacaaa agaagaagaa
     7681 atgctttccg agaaactgct tgccacagac gctgtgacaa atacgtcttt catgtccaaa
     7741 caaatcgaaa cgcaagaaga atcaatggcg aacttagact cggttgtctt aatctttgtc
     7801 gtattatcag gactgctggc ttttatcgtc ttgtataatc taactaatat caatatttcc
     7861 gagcgtgtac gcgaattatc tacaataaaa gtccttggat tttttgataa agaagtcacg
     7921 atgtacattg ttcgagaaaa tatcatcttt actcttttag gaatcatcgg aggatttggt
     7981 gtaggatata tattgactga cttcatcttg caacaagcgt ctatggagaa tgtcatcttt
     8041 ccattagtga tcacatggtg ggcttatgtc ttatcagcag gattaactat cgtatttacg
     8101 gttattgtga tgattgttac ccatttcaaa ttaaagcata tcgatatgat tgatgcgttg
     8161 aaatcgaatg aataaaaaca gaggttgtga aatcagcggt ttgacctgag tattagagaa
     8221 acaaacggca aaatagtttt tcatattttg ccgttttttg agctaatacc gaaggacagc
     8281 tgattgaaca ccatgaatca gaggttgtaa aaaagccgtc ctgcatcgag caaaaagaat
     8341 actcaaataa aattagctcc tcatgtttta catttgagaa gactgattgc cgtagataaa
     8401 agcttttgaa caccatttat agaagaaaca agaatatttt gctattttaa acaagaggat
     8461 agacaaatac atgtctgatc tcttgttttt tattgggcaa cagacctctt agaatgtata
     8521 aagataaatt ttacaaacgc tttcggttca gttataataa taaatgataa cttgtatata
     8581 taagttgtgg taggtaataa atcagttgaa tggaggtaaa tatccatgac atttaaagac
     8641 aaagtcattt accaaatcta tcccaaatct tacaaagaca gtaatggtga tggaattgga
     8701 gatttaaaag gaatcaaaga aaaactcccg tatctgaatg atctaggcat cgacatgatt
     8761 tggctaaacc ctatttatcc aagccctcaa aaagacaacg gttatgatat ctctgattac
     8821 acagccatcg atcctatctt tggtacgatg gaggaattcg aacagctaat agccgaagca
     8881 aagaaatacc ggatagaaat catgctcgat atggtgctga atcacgtttc gacagaacat
     8941 gaatggttca aaaaagcact agcaggggac cagcattatc aggacttctt cattttaaga
     9001 gaagagccaa cggattgggt atcaaaattt ggtggaagtg cttggtcgcc atttggagat
     9061 acggggaaat attatttgca cttatatgat cggacacaag ctgacttgaa ctggcggaac
     9121 caagcagtaa gagaggaact attcaaagtt gttcgttttt ggatggaaaa aggggtaaaa
     9181 ggattccgtt tcgatgttat caatgttatc ggtaaagacg aacaattgaa aaataataaa
     9241 gaaaatgatg gcaaacctga atatacggac aaaccaatca cccatacgta tctaaaagaa
     9301 ttgaaccaag caacgtttgg gacggattcc actatcatca cagtcggtga aatgagctct
     9361 acgacgatcg aaaattgtat cttgtataca gaacctagta gagaagaatt atcaatggtc
     9421 tttaatttcc atcatttgaa agtcgattat gaaaatggga ataaatggtc tactcctcca
     9481 tttgattttg cagaattaaa gcgactattt catacatggg gagaaaagat gagtgagggt
     9541 aacggctgga atgctctatt ttttaataat catgatcagc cacgtgcgct caatcgattt
     9601 gtagctatcg atcagtatcg aaaacaagga gcagagatgc tagcaaccat gatccatttg
     9661 aaccgtggga cgccctatat ttatatggga gaagaaatcg gcatgatcga cccagactat
     9721 tcatcgattt ccgactatat tgacgtagaa agtttgaatg cttataagtt actccttgaa
     9781 caaggattca cagaaaaaga agcgtttcgt cggatcaaag caaaatctcg cgataactct
     9841 cgaacaccca tgcaatggtc agacacagaa caagcaggat ttactacagg gaaaccttgg
     9901 ttaaaagttg gaggcaagct agaagagatt aatgtagaaa aagaactatc ttctgaagat
     9961 tctattttgt cctattacaa gaaattgatt cggctgagaa aaacatatcc aatcgttgca
    10021 caaggagatt atcatgctta tgaagcaaat catccggaag tttatggctt tcttcgccaa
    10081 tttgaaggac agcagctgct tgtattaaca aatttctatg ataaagatac aaggatcgcc
    10141 atcccagaag gattcacaga agccaatatt cttatcgata attattccga tactgttctt
    10201 catcaaagtc tggagctaaa accataccag gcaattgctt tgctgaaaaa ttaaaacaaa
    10261 aacacaaaac gtcgttccta cgagctattc tcgtaagatc gacgttttgt gttttattta
    10321 aaataatctt cttcagaaaa tgaagagttt tcatactctc ctttatcgcc ataaaattta
    10381 ttgtagcgtt gtttgaattt acggaaaata aaagtaacga gtaatttgac taacgtatag
    10441 ataggaatac caaaaataac gcccacaatt cccattagat cacccatgac aagtaaaacg
    10501 attaagatcg taatcggatg gatctgtaaa cgatcaccca ttactcgagg aatcaccaag
    10561 tcaccgtgga gaagttgtac ggcaaaccag acaatgacaa atttgacagc cataaaagta
    10621 gagtcttgga atgcaatgat caaacctgga aagaaggcga taaacggccc aatatatgga
    10681 atgatacata acaacccagc agaaatagct aaaacactag caaattctaa tccgatcagt
    10741 aagaaacaaa cccagtagac tgctccaagg ataaatgaag caatgatttg cccctttaga
    10801 aatgaaccca attgttgatt agcaatcttt gtcaaatttt taaaatctgt acgaaatgct
    10861 ggtgggacaa tcgctaaaac tgactgataa aatttttgag gatctctcaa aaggaaaaaa
    10921 gcgacaattg gaccagtaaa taaagtaata aaaatagctg taacagttga aaaaatgttg
    10981 ctgatccgtt gtgcgccact ttcccaataa tttccaataa atccagctat atcagacgtc
    11041 acgtcgtcaa tagaagcgaa aaattgatta aatgaatcgg caaaaggtgt tttctccaaa
    11101 aattgtcgga gactctcttg aaaatcttga aacaaatctg gcaaactact gatcaatgtc
    11161 tttgcctgtt cttcaagaaa aggaataagc catatgcttc caagccccaa taaaaataaa
    11221 ataatcacat aaacgataat gactgacaag ttcctcgaaa ggtgcttgct caaacgtttg
    11281 atgactggtt ccaaaagata atacaagatc agtgcaaaca ataatggggg caagatagta
    11341 gcgataatga ttccgacagg cttgaatata aacgagatct gatggaaaaa ataaatgatc
    11401 agcgcaagta agataactat tcctaaaaca tagtaagagg tttttcctcc taaaaagcgg
    11461 atcaaacgat tcgttgtatc tgtttctttt ttcactcttt ttcctccttt atctataact
    11521 tcattataga caaaagcggg tataaaagga aaatgataga ggttgattgc aaaaatatcg
    11581 tcaatcattc atataaactt tctcatcaat taaaataaga aaacgcttga cagttctttt
    11641 aaccatatga taaattaaat cctgttgcta aaacgtaact attacggttt gcgaaaagcg
    11701 aatcggaaca ttcaaatgaa agaagatgag aagaggagtg tcataaatga aacgattgat
    11761 aggtatattg gccatgctgg ttttagcagg tatgttaact gcttgcgggg caagtgggaa
    11821 agccgaggac agtaaagaaa aattgagcgt gatgacgacc ttttatccca tgtatgattt
    11881 tacaaaagca atcgtcgggg atgaaggaga agtagagtta ttgattccag cagggactga
    11941 ttcccacgat tatgaacctt ctgctaaaga catggcaaag atccaagata cagatatctt
    12001 tgtttataat gatgaaaaca tggaaacatg ggtccccgca atccaaaaaa cattacaaga
    12061 aggcaatgtc catacgataa aagcgacaga aggtatgctt ttacttccgg gaagtgaaga
    12121 aggacatgat catgaccatg aacacgggga agaaggccat acacatgagc tagacccaca
    12181 tgtatggcta gctccaagtt tagcaatcaa gcaggtagca aacatacgtg atcagctgat
    12241 tgaagcttac ccagaaaaac aggaagtatg gacaaaaaat gcagcagcct ataccgagaa
    12301 acttcaagca ctccatcagc tttatcaaga aacctttaag caagcaaaac aaagatcttt
    12361 tgtcacgcaa catgctgcat tcaactatct agcgttagaa tatggattga atcaagtatc
    12421 gattgctggt ctttcttcta gtgaagaacc gtctgcggcg agaattgctg aactgaaaca
    12481 ctttgttaaa gaacatggaa tcaactatat ttattttgaa gaaaacgcca aagatagtat
    12541 tgccagaacg ttagcaaacg aagcaggtgt atctttagaa gtattgaatc ctttggaagg
    12601 attgacgaat gaacaaatag aaaatggaga aaactatctt tctatcatgg aagcaaacct
    12661 cgaagcgttg aaaaaaacga cagagacaga aaacccgcta gaagatactt tgacccccaa
    12721 aaaagaaaag agcgtctaca acggttattt tgaagacgag gatgtacaag atcgctctct
    12781 ttcagattgg gcaggaactt ggcaaacagt agatacgtat gtagaagatg ggacttttga
    12841 tcctgttttt gaatataaag aaaaattgaa tcaggataag acagcacaag aatataaaga
    12901 atattatacg aagggctatc aaacagatat tgcttcaatc aagatcagtg agaaagagat
    12961 ggcatttacg tttaagaatg gaacaacgaa acgttcaaat tatcgttatg tagggaaaga
    13021 aatcttgacc tacgaggcag gaaaccgtgg cgtaaggtat ctattcgaag cagaagataa
    13081 ggaaagtggc gcttttagat atattcaatt cagtgatcac acgattgcac cggctgactc
    13141 cgaacatttt catttatatg caggaaatga aagtcaagaa gtattgctga aagaaatgga
    13201 gaattggcca acattttatc cagatacaat ggatggacgt gaaattgcac aggaaatgct
    13261 tttgcactga taggcaaaaa agaaccgaag ggtttggatc ggctggtcat ttagtcggtc
    13321 caaactattc ggttcttttt atgaaatcaa tttctttaat ccacggatag aggctgggat
    13381 ggtctcgccg tttgccaacg tgacttctct ggttttttta ttaatcgacc gcacattttg
    13441 tgtattgata acataggatt tatgacaacg gataaaattc cgatgcattt tctcgatttc
    13501 tttgatcttc tcatagaatt gtaattgggc atgagtcaaa tggacttcca gtttatgtgg
    13561 cgctcctagc gaagtagaaa aatattggat gtctttgatc ggtaaaaatt tgatctcatc
    13621 attaaactct attttgaaga tatctttttg gatttgggac atttgaaaat cttcgaaaat
    13681 tttgctcagg atatgctcga cattttcttt taaatcataa agatcttctt tgacgatata
    13741 atcagtcggt tcgatgttgc tttttaagat tttcatcgac atttccgtat gggaagtcac
    13801 atagattatt tttcctaaag gatctaattc acggatcaat ttaccgatcg ctacaccatc
    13861 catttcactg tcttctagtt cgatatctaa aaagtaaata ccaaaagaag aagttctgat
    13921 cttggctgca ttgagaagtt cttccggatc agaggtagaa atatggacat aactgtctaa
    13981 gttttcaatc atgattctat ttttgatcac cgttgataaa acttctaatt gttttggatc
    14041 gtcttcacaa atataaatgg gaatcatatc attactcctt tattttattt gtatataaat
    14101 cgtttgagaa aaacgctggt tttcaattga tgtttcaagg aaaagataag gattggtctt
    14161 tgtcaattca tccaagataa acaagccaag gccttcgttt tttggatttg tttttgttga
    14221 gaaccctttt tgtttcaact ggtttaacgg taatgtattt gttgttgtat tgatcacacg
    14281 aatcatcacg gaatgattct tcttgagtat ggtcaattcg atttttttct tctttgcagc
    14341 actagctcct tcaatcgcat tgtccaagat aataccaagc atacgtacaa ggtttaccgt
    14401 atgtttcgta tccagttcga ttggctcagg aaaatgaagc tggacgatca accctttttc
    14461 ctgtgccatc atgagtttta atgacagtaa actacggatc tcaggaactt tcatattatc
    14521 caacatactt agattggccg gaaaaaggtt catcatctgg cgtgtaggta atatcgtttg
    14581 atcataataa gaccgtaatc cttctaagtc gccttctgaa atatagctgt gaagagaaaa
    14641 aagcagattt tggtaatcat gtttgaattc tctcaagtct tgatagtttt tttctagatc
    14701 tttgatataa tcctgttgca tcctgacttg ttgctctaat aactgatcag ctaatttctt
    14761 attagagatt ttccaataat aaaccaagat gatcaggcca acacacagat atagaatgaa
    14821 aaaactcgta ttcaaagcta agacatcagg tgtttcacca gaaaaacggg tataaaaaat
    14881 aatgatatag tatgtaaata atatcaatgt cccgatcgta ccgatcatac ggcggtccac
    14941 agtgtgaaac caagtctcta acgatttttt taaacaaaaa gctaataaag cagataaaaa
    15001 agcggataag acaatgtgaa aatactgcaa tgaatctcct ggttcaataa ccgaagattt
    15061 caaaacaaaa aaatcgccga tgcttgttaa gtgatctcct aaaagcgacg tcaataaccc
    15121 aaaaggaagc gcgtaaacca attgttgacg cgaagtcgaa aagaagaaaa aagtaagcat
    15181 cagcattcct atagcaatta gagcagtaaa aatccctaaa atcgaataga cccatccaat
    15241 caaaatacaa gaaatcagaa tgactcccgc aaacagatat ttgtatcgga tgtgatggtc
    15301 aatcaataag atacaaattg tgtaaatcaa atattgcaac gctatggacc ataaaaacat
    15361 aaagtacagc tcccattgat ttttaccaaa ttatagcaca attggcgtat tattttttta
    15421 atttttcagg gattttgaat tcagaactca taccaaagca gcagccagat acaaaaaagt
    15481 cagataagtt agttaattgt ttaatgattg tttttttcat gaaagattcc tccaaaattc
    15541 atcaagtagt ttgtaatagg aagcaacgta gctatttcta aaacaatccc aagtagcaaa
    15601 taggtagaaa agtaagaagc tgagaaaaat aacgtaatga gagaaaaaat tagaaacatg
    15661 ctgatcgcct tctttaataa tgggggacgt ttctcgggtt caatgggatt gattgctgta
    15721 cctcttgatc catagcgaag cagtaacaaa aagcagctga ttaacaaaaa gaccatcatt
    15781 gggaaggaga tagtcaattg cagatgtttc atcaccaagg gaacgcccca taaataaagt
    15841 agtgtccaca gcagacaaac gaattcagag tcggaatgca atccataagc atatctgcgg
    15901 atgcagatat aagaaagaaa ggtgattcct gtttccgccc atgagcctgt caccagagaa
    15961 aagactagaa cgaccgctag ttttccaaga ttattcaata aaacttctaa gacaaatttt
    16021 acttggacat acaccatatc ttcttccgat ttgtctccgc tgaataaggc ttctaataat
    16081 ttgttcacaa ctttatcttt catctcatac gtacctccac cacaaatttt atgctaaaaa
    16141 aaataaaaaa cgtcttttgt ttcataaaaa gtggttttga catcaaaacc gtcatgatct
    16201 cctaaaaaaa tcttcgtttt ccaagaacct taccaaaaaa agtgacagtt ttgatacgaa
    16261 aagtactttt tatgacaagt ttgtgctttt tcgagtgatt tatgttaaaa aattactgac
    16321 cttataaaag gtgatggagg gattcttatg gaaaatgaac aattgtttga agtcggcgaa
    16381 acatatgcat ttaaatctga tttatttgaa tcaccaataa aaggaaaaat agaaaaaatc
    16441 tatgaaaata gtgtgatgat acgtgtcatg aaatgcgcag tagaagacaa tgataccgct
    16501 cataatctgc actggcagtt agttgtacgc aaatcaagta caatagaaga ataaaaacag
    16561 ataaaatgtt gagagcataa agaggttgtg agtctcccgt ttagctctga gttacaagga
    16621 ataatgttaa aaaacagctt ttcatgtttt atgaatattg ccttgtgacc gaggtgttgg
    16681 caggagaata ctgtttattt gaaaaaaggg ctgcgacaag acttttgtca cagcctttat
    16741 tttcatgaat aggagttact tttggctact tttagagaag tagcttttaa ggagcagata
    16801 aagtcaaggc ttttatttct ataaatgaag tagtaaaatg aagaacagat tttattacag
    16861 gaaggatgcc gataattgtg atcattgttg caggaacgat cggtgcaggg aaaagcacac
    16921 taactgaaat gcttgcccaa gatttagaaa caaaaccttt ttatgagaac gtggaagata
    16981 atgaagtatt gccgctattt tattctaatc cagaaaagta tactttttta ttacagattt
    17041 tctttttgaa caaacgcttt ttggcaatca aagatgcttt cagccatgat gacaacgtac
    17101 tagaccgctc catctacgaa gatagtatgc tgtttcattt gaatgcagat ttagggcgtg
    17161 tttctgaagt agaagtaaag cagtatgaag gtcttttaga aacgatgctg aaggagttag
    17221 aagaaatttc gccacaaaag aaaccagatc tactggttta tattcgtgta tcctttgaaa
    17281 ctatgttagc gagaatcaaa aaaagaggcc gggaatatga acagctagaa caagatcctg
    17341 aactctattc ctactataaa gaactcaatc gacgatacga agagtggtat gaacagtttg
    17401 atatatgtcc caaaatagtg atcgatgggg acaaatatga tttcgtagcc gatccggcat
    17461 gtggagaaca aatcgtacaa gaaatcaaga tgcgggcaaa gaaaatgaca gaagaggcag
    17521 atgcccagtc gatccgctac actacaaata acccgaaagc aaagggaatc tttacaacta
    17581 aataatataa aaactaagca aattgctcac tcgcagatat ttctctgtga gcagcatctt
    17641 gcttagtttt tttgattcat tgagatcaaa tagtgaaccg attcgtatga taaaattgcc
    17701 ataaatgaaa tgtctgagtg agggccatga tcaaaaataa aatggaaacg atcaaaatcg
    17761 aatgggcgcc aaacagatga tgacagaaaa caaggacaat agctcctgcg ataaaggcaa
    17821 gaacgacact tagatagtgg aaaaatcgat caaaatctgc tttttcttta gaaatacaat
    17881 aatcatataa gttcgttgct acccctttta aatttccagt tgtaaataaa ttagtgtagg
    17941 cacgcccgtt gattttatca aaagctaccc actggatcga tgcagtaaaa gcaatcaaaa
    18001 tcgttactaa tgcgggatgc tcatggacgg gtgtcaaact aacaaagaaa aaaatcactg
    18061 cttcataata tagaacaaac aagcgccaga aatgtttttc tttttttcta aaataatcaa
    18121 ttagatactt tgttagaaga atccctaaga aaaagaaaag ggtagacaat acttttttac
    18181 caacacttag ccattctccg tcaaatgctt ggatgatcga tagaatgata ttgccagttt
    18241 gagcagaggc aaaagcacca tattggatat aagtataact gtccatggct cctccgataa
    18301 atgtcagcaa aagtcctatt gtacgcccct cgtgaaagga agtctgcatt tttttcatca
    18361 ccttttatga atatttttgt cttccttatc ttagcataaa cgattgaaaa aaaggaaagt
    18421 tttcagaaaa ctaaaatcat atcgcttccc attttcattt ttagaaaggt ctgttagtat
    18481 aaagaaaaaa tagacaacga aactttaaat aaccaacttg aaataaacaa attcaaagaa
    18541 gaaagatggg aaggaaatca atcagttgaa tgaaaaagaa attctccgat tacaaaaaga
    18601 attgaaacgc tgggagaaca agtataaaaa gcttcaacaa gtctttcggc tacaagaaga
    18661 aatcattgct acctatgaag cggcttatgg cgaagcaccc ggacttattg aggaagaagt
    18721 acaagtaaaa gaaagtgaag gaaacagctc tttctttaaa atgatggaga aacgttccta
    18781 taatcgcttc aatcaagaac aaaaagcagc tatcacatat gatatgaaga aacatcttcg
    18841 aatcatagca ggagctggga gcggcaaaac gcaaacaatc tgtgcaaaag ctgtttatct
    18901 gatgacgcaa aaacaagtag acgaagaacg catactaatg atcactttta caaggaacgc
    18961 tgcaaatgaa ctaaaaaaac gagtagataa tttcagccag agaaaaaccg ctgtccatat
    19021 cggaacattc catagtatct tttttaggat atacaatgaa atttgtcgaa aatttcctga
    19081 agtagcaatg caaggaatac aaggagactt ttcaaaggat actgcacaaa aagtgaatgc
    19141 tgttttgcaa cagctgatac gtaaatataa tctttatata tttgatcaat atggagaaaa
    19201 aacgattgct tctcggttag actattggca gaatatgaac ctttcagtag aagaaatggt
    19261 tcagttagtt aaagaaaaat atgattccat tgataagaat gcacgacagc cgatatcaga
    19321 acgactgtat ggattgctca cggaactcca agaacaaaaa agaagccagc aattgctaga
    19381 gttcaatgat gtactccaaa atctgaaaag cgctttggag aatgaggaga tccagcatta
    19441 tatcggccaa aaatatgatt atctattcat tgatgaattt caagatacta atccacttca
    19501 atgggaaatc gtgaaacgga tgacaaagga aaacaggatc aagttgattg ttgttggaga
    19561 tgatgatcag agtatctatg gattcagagg atcagagcca gcatacatca agaactttga
    19621 aaaagaatat cctaccaaaa ctcttttctt attaactaat tatcgttctc gggcagctat
    19681 tgtccaagga gcaaatagat tgatcagcta taataaaaat gatcggatac caaaggcgat
    19741 gattccagca caaaaagaag caggtgttat ggaagcctat ttgttttctg atactaaagc
    19801 agaaagtcag tggctaatca aacaaattca gacatttatt caaaaaaatg ggaaatataa
    19861 ggaatcaatc atattatacc gatcagcttc acaaacgcag cagcttgtcc aatctctgct
    19921 gaaaacgaat attccttttg tactggaagc agatagtcca tatgaaggta tctttggtat
    19981 caagtctttc caacatttct atcaaaaaat tgttcattgg caaacagcac tgaatctgca
    20041 aaagaaaaat caggcctacc agcaattgct ccggcagctg atggcagact gctacctgaa
    20101 aaaaagtgaa tgcgatcaat acttttctcc taaatatcaa aatatagccg ttgcagatta
    20161 tattttgtcg ataagcccca atttaaaatc gaaatctgca gaaatccgag agtttgagga
    20221 agcgttgcgt aaaacagacc agcatcaaga aatctattcg cttgttcaaa gctatcttca
    20281 tctgccacgt atagctaagg aaatcgacca gagtgatcaa gaatggatac gagaagaagt
    20341 aaagcagcat gctactttca gaagactcca agccttatcg caagaaaccc aagcgacagc
    20401 taaagaatta aaaacccgct tgattgctta cagacaagga aaacttgatg cactatgtat
    20461 ccagagcatt cacaaaagta aaggtcttag ttaccgaaat gtctttttga ttggttgtaa
    20521 cgaaggaagt ttgccttata acggagcgac agaaaagaaa gtaatcgact ttaatgaaat
    20581 aaaagcagaa ccagtaacga cgatagaaga agaacgaaga ctattttatg ttgcgatgac
    20641 tagagcaaga aatcgtctgt atctatgtgt acctcaaatg aaaaattcaa gaaaattgaa
    20701 agtatcgaga tttatcaaag aaaccgggag tcgtgtgaaa aaaggaaagt gatagaagag
    20761 agggtgtgac agaagtggtc agtttcaaga aagaagaagg aatgtacgaa aattgcttct
    20821 caaatttttg tgaaatttca gcttattttc gaagaagcta tttctggaac acagtttatt
    20881 cggaaaaggg aggtgtgaca agaattttgt cacacctccc ttatttattt tgaaaatgag
    20941 aatactatta tcaaaataga gaatatatag aaaaattctc attattcaaa cgtaaataat
    21001 cagttatttt gatttttttt atattttatt agctaataat tggtatttta aaagaaatta
    21061 ctgtagaatt ggattataat taaaaaggag tgagaaaatg ataaaagtat atacttcgcc
    21121 tagctgtact tcctgtagaa aagcaagagc atggttgcaa gcgaatcaac ttgaatttga
    21181 agagaagaat atttttgccg aaccgttaac agaaagtgag atcaaaaaga tcttggcagc
    21241 gactgaagga ggagttcaag atattatttc cactcggtcg aaaatttacg aaaagctggg
    21301 catagatttt aacgaattaa ctttaaagca aatgatcgtc ttattcaaag aatatccttc
    21361 attattgaga cgacctattt tgattgatga aaaaagaata ctaatcggat ttaatgaaga
    21421 tgagatccgg tcatttgtcc ctcgtgaaat tcgatgtgtt gccagaaatc tgatctattt
    21481 acaaaacaat aacatcttaa attcataggg agggagcctt cgtggaagtg aaagtctatg
    21541 gatcagcgca ctgtgtaaca agtctgaagg cgaaacaatg gttagaacac catcaattgc
    21601 cttatcaatt tgttgactta gaagaacgcg atttaagtac agaagaagca gaagaattat
    21661 gcagttttga agaagtgcaa gcagaacagc tatttgcgac ttggtctgag gcttttcaca
    21721 acattgcgat tgacctttcc tgtgtaggaa aaaaacaact gattttttta tgcacacaac
    21781 ataccaatct cctcagacgt cctttgatta ttatcgatga agttctattt attggataca
    21841 atgagcagtt gatggaacag cttatcttaa ataaatagac tttaactgtt ttaatcaagt
    21901 cactgagcat ttcatacagt tgtaacagtc gaacacttag catcaagcgt gttatgtgtt
    21961 ctttttttga ctttattttt ctactataga gacatgctca atttatgtgg tcaaaattca
    22021 aatctaggtg ggga
//
LOCUS       DAZKLD010000037        21890 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.037, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000037 DAZKLD010000000
VERSION     DAZKLD010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21890
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.037"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(297..410)
                     /locus_tag="ACG8KR_001136"
                     /pseudo
     CDS             complement(297..410)
                     /locus_tag="ACG8KR_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(793..2025)
                     /locus_tag="ACG8KR_001137"
     CDS             complement(793..2025)
                     /locus_tag="ACG8KR_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFQ0200520.1"
                     /translation="MLSDFNELLKKYARLIAETGVATEKGHTVVLQISVEQAPLARLI
                     TQEAYKLGAAEVIVQWTDDQIQREFLLHAATDRIENVPQSKIDQADEWLEKGASRISV
                     VSADPDAFAGVDSHRVASYQSANGKALMNLRKATQANKVSWTVVAAAGKQWAAKVFPE
                     LPEEKQVEALWDQIFKTTRVYEEDPVLAWKKHDEKLAKKAEELNREQFSALHYTAPGT
                     DIIIGLPKNHLWEGAGSYNARGEKFMANMPTEEVFTAPDSHRVDGYISSTKPLSYAGT
                     IISGMKFTFKDGKVVDFSAEQGEDVLAKLLDTDEGARRLGEVALVPDPSPISQSGIIF
                     FNTLFDENASNHLALGSAYAFSVKGGTEMSDEELAEAGLNRSQTHVDFMVGSDKMDID
                     GIREDGSTVPIFRNGDWA"
     gene            2278..2847
                     /locus_tag="ACG8KR_001138"
     CDS             2278..2847
                     /locus_tag="ACG8KR_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFQ0200521.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLY
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
     gene            complement(3025..3465)
                     /locus_tag="ACG8KR_001139"
     CDS             complement(3025..3465)
                     /locus_tag="ACG8KR_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HFQ0200522.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLLGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            3623..4387
                     /gene="map"
                     /locus_tag="ACG8KR_001140"
     CDS             3623..4387
                     /gene="map"
                     /locus_tag="ACG8KR_001140"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HFQ0200523.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            4419..5342
                     /locus_tag="ACG8KR_001141"
     CDS             4419..5342
                     /locus_tag="ACG8KR_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HFQ0200524.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFNKLKTKFSHKKK"
     gene            5418..6557
                     /locus_tag="ACG8KR_001142"
     CDS             5418..6557
                     /locus_tag="ACG8KR_001142"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HFQ0200525.1"
                     /translation="MLVSFSMVIRFFLTILLSLILTPVFKIISVQTGMVDKPNERRIN
                     KVPMPSAGGLPIFISFVISTLFLFRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPK
                     QKSIGILIASLIIYFVADIRIDSFTLPFIGYIQLGWLSFPVTIFWIFGITNAVNLIDG
                     LDGLAAGISLIGLITIGIIGYFFLHASTVYIPVVIFCLVASIIGFFPYNFYPAKIYLG
                     DTGALFLGFMMAVLSLQGLKNVTFVSSISLLIVMGVPVTDTFFAIIRRKANRVSFSTA
                     DKKHLHHRLISLGFTHKGAVLTIYAMALMFSFTAMVMNYTGRIGTIILIIAMLFAAIL
                     LFELIGLINEKHQFILKTLRFLGNKEYRTQVKQKYLKKWNRHIDE"
     gene            6550..7350
                     /locus_tag="ACG8KR_001143"
     CDS             6550..7350
                     /locus_tag="ACG8KR_001143"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFQ0200526.1"
                     /translation="MNNKLNQPILISIVTANSDKIFRTLDNFISTVKDETAVKIRIFD
                     NNSLPEYKKRLTEYRKYPYIDIYFHDENSGFGYGHNHNLIASDSRYGIICNPDILVDE
                     ETVTALVLLLKEHPECAMLAPKILNEDGTTQHLIREQLAVFDYVLRFVPFKWVKKKFD
                     KRLAVFECRGLPTDANSYVRMISGSFMVADIDKFKEIKGFDERFFMYFEDNDLCLSFE
                     KAGYKLLYTPFYAVIHFYGKGAHRNAKLFRVFLTSMMKFFNKWGWRFF"
     gene            7350..8177
                     /locus_tag="ACG8KR_001144"
     CDS             7350..8177
                     /locus_tag="ACG8KR_001144"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFQ0200527.1"
                     /translation="MKPEILVVVVLYQQLFSQSPAYGPLSKALSEKQIQLIIYDNSPQ
                     RQQNELFEKENIVYHHDPQNPGLAVAYNFALSQADEGTRCLVTLDQDTRLVDDYFELL
                     RKVTFTDECVAAVPMIFSGSRQVSPLYSDRYINRTAQAVEVEGPTAERIMAINSGVAW
                     SVKFLKKIGGFNPAFSLDFLDHWLFWKVDQLEKTVEILPVRMEHDLSVLDYEKVSSNR
                     YRSILQAENRFYHEYDQRHLSSHRRQLLLRTAKQFLTVKNRHIWRMTLRSYVKNWKV"
     gene            8182..8889
                     /locus_tag="ACG8KR_001145"
     CDS             8182..8889
                     /locus_tag="ACG8KR_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFQ0200528.1"
                     /translation="MRSVCIATFNGGRYLREQLDSILSQLSAEDEVVISDDGSVDDTW
                     DILTEYAEKDKRIQLFSGPRQGLIANFGYAIQQTKGDLIFLADQDDVWLETKVKKITD
                     YFEQNPDQLVVVSDLIIVDSELQEIHDSYFSFRNSKEGWLNNVIRSHYIGAGMAFRSS
                     LKPDILPIPSEVPMHDMWIGLLGGKKTGFLREPLTLYRRHDLNASEIETHSKLRQKIS
                     WRVHLITALIKRKWFKR"
     gene            8989..9855
                     /gene="rfbA"
                     /locus_tag="ACG8KR_001146"
     CDS             8989..9855
                     /gene="rfbA"
                     /locus_tag="ACG8KR_001146"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HFQ0200529.1"
                     /translation="MKGIILAGGSGTRLYPLTKATSKQLMPIYDKPMIYYPMSTLMLA
                     GIKEILIISTPQDTPRFKELFGNGNDLGLQIEYAVQESPDGLAQAFIIGEEFIGDDSV
                     CLVLGDNIYYGGGLSKMLQRAASKDTGATVFGYHVNDPERFGVVEFDENMQALSIEEK
                     PAQPKSNYAVTGLYFYDNEVISIAKGIKPSERGELEITDVNKAYLDKGKLSVELMGRG
                     FAWLDTGTHESLLEASTFIETIEKRQNLKVACLEEIAYRMGYIDKEQLLALAQPLKKN
                     QYGQYLLNLAAE"
     gene            9869..10441
                     /gene="rfbC"
                     /locus_tag="ACG8KR_001147"
     CDS             9869..10441
                     /gene="rfbC"
                     /locus_tag="ACG8KR_001147"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HFQ0200530.1"
                     /translation="MKVTDTKLTDVKIVEMDVFGDHRGFFTESYSKAKFAEHGLKYDF
                     VQDNHSLSAEAGVLRGLHFQKGEAAQTKLIRVVTGAVLDVVVDIRKGSPTYGQWDGFI
                     LSEHNHRQLLVPKGYAHGFVTLTPNVNFLYKCDNYYNAEADGGIAFDDPDLAIDWPID
                     PSRAIMSEKDHRHPTLKEFEAENPFVYGEI"
     gene            10463..11491
                     /gene="rfbB"
                     /locus_tag="ACG8KR_001148"
     CDS             10463..11491
                     /gene="rfbB"
                     /locus_tag="ACG8KR_001148"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HFQ0200531.1"
                     /translation="MKNIIVTGGAGFIGSNFVHYVVNNHPEVHVTVLDKLTYAGNKEN
                     LAGLPSDRVELVVGDVADAELVDRLVKEADAVVHYAAESHNDNSLKDPFPFVQTNIIG
                     TYTLLEACRKYDVRYHHVSTDEVYGDLPLREDLPGHGEGEGEKFTAETPYNPSSPYSS
                     TKAGSDLLVKAWVRSFGLRATISNCSNNYGPYQHIEKFIPRQVTSILSGIRPKLYGEG
                     KNVRDWIHTNDHSSAVWLILTKGRIGETYLIGADGEEDNKTVMELILEMMGQPKDAYD
                     HVNDRAGHDLRYAIDSTKLREELGWQPEFTNFRDGLADTIKWYEEHEDWWKKEKEAVE
                     AAYAKNGQ"
     gene            11589..12440
                     /gene="rfbD"
                     /locus_tag="ACG8KR_001149"
     CDS             11589..12440
                     /gene="rfbD"
                     /locus_tag="ACG8KR_001149"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HFQ0200532.1"
                     /translation="MFLLTGGNGQLGTELRHLLDEKNLPYVSTDAKDLDITDAEKTMA
                     YITELKPDVIFHCAAYTAVDKAEEEAKELDEKINVDGTRNVALAAKAVNAQFVYISTD
                     YVFDGKNKNVEYEVDDQTNPLNEYGRTKLLGEKAVEEILEDYYIIRTSWVFGIYGHNF
                     VFTMQRLAETRDKLTVVNDQFGRPTWTRTLAEFMVYVIEEKAPFGIYHLSNENSCSWY
                     EFAKEILKDTDVEVAPVTSEEFPQKATRPQYSVMSLKKTEALGFVIPTWQEALAQMLE
                     AAKKMNK"
     gene            12475..14508
                     /locus_tag="ACG8KR_001150"
     CDS             12475..14508
                     /locus_tag="ACG8KR_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0200533.1"
                     /translation="MEKQTRSARRKEKPKANQADSNLVLDLFLKLRYLIGVLVIVVIV
                     TFNLNGSSLGVWDKYVSQRDDGKKTDVIFGQNREVRSDEWLVQTPFYLSQAEEDFSLV
                     NEDYSLNGQNMIIAYNSPVKDITVIGKPFNWGFFFLGRDRGLSFYWAMKLVVMVLLGF
                     EVLMILTKRKKALSLIGAFALTFSPAVQWWFMQHVGDLIFFTLGLMIAFYHYFYNHDK
                     KWLRTLMMLLVVIFGIGFILVIYPAHQVMLAYLLIFYFVGLLIYYSKKVSWDLLDVVL
                     IGGAVLFIGGVMVHFWLTSKDALLASLNTLYPGKRVSTGGKWTIGEFFSFLTNWKIPF
                     EDINYSNNSEVALFYHFFPTVFLASPFVLFGKKDSEQKLLGRILMIFCLFSIFWITVG
                     LPKWLAEITLLSYVPPLRAILTFSFAACLLSIWFIAYIWREDVIPIWYKFILLAVNAW
                     SYFYAITHSKMDNYFSDFETIAVAVLGTVILAFALFGWRRLFYLAFVALVIVSGFFVN
                     PVVEGTGAIFEKTLAKEIQKIDQQDPGKVWLSEDELYNFTPTLGVKSFNTVRFYPDIA
                     AWKKIDPKEEYEKYYNRYAHTRAFIAKEDTKFILDRPDMFSVTLNFEDAEKLGIDYVI
                     SKRPLTDYNQLYEAQFTQLYGPDKDGYMIFKVEYPDYPDVPQTQTQENQLVVQ"
     gene            14552..15832
                     /locus_tag="ACG8KR_001151"
     CDS             14552..15832
                     /locus_tag="ACG8KR_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HFQ0200534.1"
                     /translation="MKIIQAIVQKFVNKMSVGFLLLLLTLVFFVWTAGVGFPATLFIY
                     NNSSLLIAFSVAILLILNVYKAKGVDFLYLGVALALFIFFSYFRELRNESLFGDLLFP
                     LIILFCLVIKWIEWTKWDRGIYLIVFTVFLGITLYRVFTEIQVPEGQSIWAPGNSLSD
                     IWINTNTIGSSLMTLALLISGFASSFERWYVRIIGGIAVVAAFLGIWVCQSRGALVAL
                     IVFAILDLLPKSLMRVIRAPFIAYTVTILLALPISYLAAVSEKVNLFTGREDIWHKFY
                     QTLGEKSEQVLLGMKTFIFQRGNQFLGNHNSYNSILNIYGLIGFGIAALLLILFIGRL
                     TLKADLSNGQMTFIWAFFAVMMQSFMEDTLTSYRWVPIIYILLAMAVHRYDEPEVTEE
                     FYVMEEAYDDMTYDDTSYEEQPDSAHSSRIQRYH"
     gene            16042..16848
                     /locus_tag="ACG8KR_001152"
     CDS             16042..16848
                     /locus_tag="ACG8KR_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0200535.1"
                     /translation="MFKDLYLFMKDIYQNRKLLLQFSINDFKSRYAGSFLGILWAFIN
                     PLFTVLIYWLVFGVGLKAQMIDAKYPFIVYLVTGMIPWFFFSDAFASTTVVFREYTYL
                     VKKVVFNIRILPTTKILSNLYTHLFFILIGIAVALINGIYPSAMSLQLIYYLFCMCAF
                     LTGLTWLTASIQPFLPDIMQLITIVLQLIMWTLPILWSPNQFDSGLVSILKLNPLYYV
                     VQGYRESFLSEAWFWEHGTYTLYFWVFTLVMFVIGSAVFRRLKPHFSDVL"
     gene            16860..18080
                     /locus_tag="ACG8KR_001153"
     CDS             16860..18080
                     /locus_tag="ACG8KR_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0200536.1"
                     /translation="MTEYAIRLKDITKSYNMYAKPSDRFREALNPFKKSYHDVFYALK
                     NVNVEIKKGEMIGFVGENGSGKSTMLKIITGVLTPTSGTMEIEGKISALLELGSGFNP
                     EYSGYENIYLNGMVLGFSREEVDEMVDDIIEFADIGDHIYQPVKTYSSGMFVRLAFAV
                     AINVNPDILIVDEALAVGDLEFQLKCMEKFTEIKNSGKTILFVSHDISSIRRFCDRSY
                     WLKNGKVVEYGDTMEVTTNYENFLKKKSVKTVDRNKNQVERFAAPDIVDVTKAELLDK
                     DKNPVDMLEQGEKLYVKVTYEVKDDTIKNPVLGIALRTVDNFYVCGLNTLLDGIKIPW
                     KKGENIFYLEYEKLGLLAGEYYFDVAVFEENATVPLVYKTKYMNLFVSGSYIGEGIVV
                     LDHKWEEGTHSNEI"
     gene            18070..19656
                     /locus_tag="ACG8KR_001154"
     CDS             18070..19656
                     /locus_tag="ACG8KR_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFQ0200537.1"
                     /translation="MKYDFEMDLDEQSSVGKIAAQIKPGSKVLEFGPGNGRLTKHLIG
                     AKQCEVSIVELDKELFDFVSEFAQDGFYGDIESFEWANYYAGQTFDYVLFADVLEHLV
                     DPGKTLKKVREFLNEEGEILITFPNLAHNSVMIDLFNNQLPWASYGLLDETHNSFYTH
                     DGFQKVFEKAGLYINIEDYLYLAVGDTELKSTYEELPEAVRYDFKMRPFGEVYQYFFS
                     LMKHPVAQSSIAQPQNSNYVKVLEVIQQTKQDEIIQQIPFNNFTGENETLSFHVSELA
                     ERMVFRFAQQPSFIEFSAEAAGEKLTFIDSNAVVKTVNDCYLFDGKDLPEFFLTDIAG
                     KEVTIHCHYRFIGELTPTMKELLETIRPMAEVTKQLSEKNDELERENHYLIENNTRLD
                     QTLKTTINRYCTLLESDEWTIKHRLGRRKKETSKKIQEKELSLCIDEKIWDAETKILK
                     IIGWGIANSDRQPLSYKLSADQSPFFEAIPVLREEVNQAKQLAEGAKAGFELRILCEK
                     ERSFLVEAVAQNGQSWIIEM"
     gene            19695..21833
                     /locus_tag="ACG8KR_001155"
     CDS             19695..21833
                     /locus_tag="ACG8KR_001155"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFQ0200538.1"
                     /translation="MQKKEIAVFIDQITRERATGDLLIVGWAIDEVTKEIPTIKVEKE
                     NVIAEATHVVRLDINHLYNLDVKTQSGFKVRLSGKMRGKAILDFQTARHQNGIAVKLN
                     GKYPYDDGIESSWERKKRLLKKGINYARTHGVKKAIRRVKLELKPGSIDYAEWISRHE
                     KPDLKEQRKEVQGFAYRPLISILMPVYNVEIKWLEKCIDSVLDQTYDHWELCISDDAS
                     TDPAIRKCLESYQAKDDRIKVVFRQENGHISLATNSALEMAEGEFIALLDNDDELPPF
                     ALYEVAKVLNVHPELDLIYSDEDKIDADGNRFDPHFKADWSPDTLMGNNYISHLGVYR
                     TSIVKELGGFRKGYEGSQDYDLVLRVTEQIPAEHIYHIDRVLYHWRTIPGSTASNGEA
                     KSYIYDSGVKALTDALSRRNIKGSVHPGRISGFYEITYDVLQEDLVSVIIPTKNGYED
                     LKTCVDSIIEKTSYPNYEIIIADNGSTDPKMQELFAEYKHQLKDRFIVELIDIPFNYS
                     RINNLAAEKANGKYFLFLNNDTEVIEPDWMTAMVSYAQFDRIGCVGAKLFYPDDTTQH
                     AGVLLGIGGVAGHALNNYDRTHCGYFGRLVIDVNYLAVTAACMMVKAADFNAVNGFDE
                     ILEVAFNDVDLCLKVYELGRYNVYAHQAELYHFESKSRGYEDTPEKQRRFAGEIKKMQ
                     DKWPSYIAHDPFYNDNLTKEGIGDFSLRPD"
BASE COUNT         6905 a         3707 c         4548 g         6730 t
ORIGIN      
        1 aaatttaaac cataattgtg tttttccttc catcagcata aaagttcacc tcaaaaagag
       61 tatacgaacg aatgttcttt ttgtgaaggc ggaatttaat atactgaata atagaaaaca
      121 agtacttgtg ccaaattatg tgccaaaaaa attcgaattc aacaaaatgt aaacaaatag
      181 aaaaccttct atattaagat ttcctgcaat aacaaacaca acagaagacg tgtaatagtt
      241 agtcaggaac gtacaaataa cccctgtatc ctttgcggta cagggggtta ttttgtataa
      301 gtagacattg tagtttctaa actgctatgt cctaaacgtt tcgatacgct aagtatattt
      361 actccttgat aaagtaaaat agatgcatgc gtatgtctta gtccatcaat aaacagtcga
      421 agagaaaata gggaaaaatt gattactaaa gaaaaactta ctaggtcgac ggtgtagttg
      481 attttcatac ttcaatagat tcgaattaaa cgatttcgat tggcttatac cagtcctatc
      541 caacgaaaga tatatcgttc ttaactctag cggtataact tgaagcttta cgtaaagcca
      601 atgggaatga actggagatg actttttatt atttttagta atagctgatg ggttaatact
      661 tacaactttc cttttcaaag taccatataa aaaaacggtg gggttgttta gcgaaataaa
      721 ggatctaagc caatagctta gatcctttat tctatgcttt atattatttt ttgcatgtgt
      781 gcgaaagtat actcaagccc aatcgccatt tcggaaaatc ggtactgtag agccatcttc
      841 acggattccg tcgatatcca tcttgtcaga tcctaccatg aaatctacgt gggtttgact
      901 tctattcaat ccagcttctg ctaattcttc atcggacatt tctgtaccgc ctttcacact
      961 gaaggcataa gcagaaccta gtgctaagtg attcgatgcg ttttcatcaa ataatgtatt
     1021 gaaaaagatg atgccagatt gagaaatagg tgaagggtca ggtacaagtg cgacttctcc
     1081 taaacgacgc gcaccttcat ctgtatcaag cagttttgct aagacatctt ctccttgttc
     1141 ggcagaaaaa tcaacaactt ttccatcttt aaaagtaaat ttcatacccg agatgatggt
     1201 gccagcataa ctcagtggct ttgtacttga aatgtaacca tctactcgat gactgtcagg
     1261 tgctgtgaac acttcttccg tcggcatatt tgccatgaat ttttctccac gagcattata
     1321 gcttccagcg ccttcccaaa gatggttttt aggcaatccg ataatgatat ctgtaccggg
     1381 agcagtatag tgaagggcag aaaattgttc tcgattcaat tcttctgctt tttttgctaa
     1441 tttttcatca tgcttcttcc atgctaagac agggtcttct tcatagacac gagtcgtttt
     1501 gaaaatctga tcccataatg cctcaacttg tttttcttct ggcagttctg ggaagacttt
     1561 tgctgcccat tgtttaccag ccgcagccac gacagtccaa ctgactttat ttgcttgagt
     1621 tgccttgcgt aaattcatta gtgctttgcc attcgctgat tgataagacg ctactcgatg
     1681 gctatctaca ccagcaaatg catcgggatc ggcagaaaca acgctgatcc tgcttgctcc
     1741 tttttcaagc cattcatccg cttgatcgat tttagattgt ggtacattct caatacgatc
     1801 cgttgcagca tgtaaaagaa attctctttg gatctggtcg tcagtccatt gaacgataac
     1861 ctcagcggct cctagtttat aagcttcttg tgtgatcaat cgagcaaggg gtgcctgttc
     1921 cacgctgatc tgtaaaacaa ctgtgtgtcc tttttctgtg gctacgccag tttctgcaat
     1981 taagcgtgca tattttttta gaagttcatt aaaatcagat aacataataa aaattcctcc
     2041 tcaccatttt ttctggtaac tattagcatg ataccattta attatgattc aaacaatgag
     2101 gctttaaata aaaaaaggag gttatttttt aaggttaagt ttcatgatat atgttactac
     2161 ttgctatttc agcttgtgaa gaaaatcaaa aactaatttt gcatatcaaa aaaacatgtt
     2221 acaatgaaat taatcaatgg tggaagcgtt ataaaaaaat taaggagatt aaaaaatatg
     2281 gcaagaaaaa agacgattac tagagaacag attttggcag ctgcttatga agtagctgcc
     2341 accgatggat ttacacgatt tactgcgaga aacatcgcga acaaaatgaa ttgttccacg
     2401 caaccgattt atcttgagtt taaaaatatg gatgacttga gacaagctct ttttgagcag
     2461 atctatgatc atttacaata tgaagtgttc actgttgagc atacagggaa tacgattatc
     2521 gatcttgctt ttaattatat ccattttgca agaagagaga agaagttgta tcgttcactg
     2581 tacttagaag aatatggtgg aggaaaagag atgcagaatt tctcttatac ttactttgtg
     2641 aatacagtga aacgcgatcc agcttatgtg aatctttcag aagagcagat cgaatctttg
     2701 tataatggga catggatcat agctactggg atcgcagctt tgatgtcttc aagcatcatc
     2761 catccaactg atgaacagat tgaaaagatg atccaagaca gtatagatgc tatcttgaaa
     2821 ttcaaagaac caatcgaaat cgattgaata tactcaaaag ttccacaaat cagtaggtaa
     2881 caagagatgt gtcaaaataa ggaaaagttc gtttttctta cagcgtatgc atttttaact
     2941 cccgaagaac tcttccatga atgcaatgaa taaaagagcc atacagattg aataaatctg
     3001 gatggctctt ttattgcaag tatattaaat ttttgcgact agttctttag cgaaagcttc
     3061 taaattttcg atatcttctt cttcagcatt cagatccact ttaacatttt cggctccttt
     3121 tgatgcgcct gtttttgcaa aagcagcatc gaaatcatcc accgatttgc agaattcatc
     3181 gtaaaaagta tcaccagatc cgcagacacc gtaaattttt cccaatagat ccaattcttg
     3241 caagtcttca taaaagtcta cgatctcatc aggcagatcg ccatcatcgt atgtataagt
     3301 agctactacg caaatatctg cctcctcgaa ttcatctgca tctacttggg tacattcgtt
     3361 gatttcgact tcgatatcca gattttcaaa tgcttctgca acaatatcgg cgatttcttc
     3421 agtgtttcct gtcatgctag catatacgat ttttgctaaa gccatgatgt gcctcctcgt
     3481 ttataattat ttaacaatta tgaattatac caaaacatca ttgaaaagaa aataaaacct
     3541 aaagacacct ggggaatgtc atttggctaa atgtatgccg ctatgttaaa atgagaggga
     3601 tttgtaaaaa ggagacgaaa atatgattac tttaaaatca gcacgtgaaa ttgaaatgat
     3661 ggcagaatct ggagctttat tagcagatgt acataaaaat ctgcgtgatt ttataaaacc
     3721 aggaatcaca agttgggata ttgaggtatt tgtcagaaac tatatcgaga gtcatggcgg
     3781 aattgctgcg caaattggct ttgaagggta tgaatatgcg acttgtatca gtataaatga
     3841 tgaaatttgt catggattcc ctcgtaaaaa gcctttgaaa aacggtgatt tggtaaaagt
     3901 cgatatgtgt atcgatttga aaggcggcat ttctgattct tgttgggcct atgtagttgg
     3961 agaatctaca cctgagatcg accgattgat ggaagtaacg aagaaagctt tgtacatcgg
     4021 tattgagcaa gcacaagtag ggaatcggat tggtgacatc ggtcatgcta tccaaactta
     4081 tgtagaaggc gaaaatctag cgatcgttcg tgattttatt ggtcatggcg ttggtcctac
     4141 gattcatgaa gaaccagcag ttcctcatta cggagaagct ggaaaagggt tgcggttgaa
     4201 agaaggcatg gttatcacga tcgaacctat ggttaatacc ggaacttgga aaatgaaaat
     4261 ggatcctaac ggctggactg cgtatacaag agacggcgga ctttcttgcc aatatgaaca
     4321 tacattggct attactaaag atggtccaag aattttgaca tcacaaggag aagaaggcac
     4381 ttattgatct atccagtcaa taggtacggg agaggtacat gaaagtttgg aacaaaataa
     4441 aacaaaatga aaatctgatg cgctttattg agacgactca aaagcgcatc actgattcag
     4501 aaatgagtac gacctctgtc gtcgttgctt attatttact cttgtcttta tttccattga
     4561 ttattgcttt tggtaatatt ttaccgtttc tccacatcga tcaagagact gtactgacct
     4621 atttacgaga agtgattcct gagaccgttt accagtttat cggtcctgca atcaaagacc
     4681 ttttgacaca aagttcagga ggcttgttat ccgtttctgc attagcggct ttatggtctg
     4741 caagtcaaag tatcaatgct ttgcaaattg cgatgaataa agcgtatggc gtagaaaata
     4801 gaaaaaattt tttgatcgtc cgtttcttct cactaattgt cattgtcttg ttcatgattg
     4861 ctattagtgg ggtaactatc gttcttgggt tgggacagct gattttggat gctattcagc
     4921 caatcgtaca aatacccgtg aactttatcg atcagttcca aactttgaag tggccaatca
     4981 cattggtagc tttatttgtc atcatgttct tgatttattt aattgtccct aatgctcagt
     5041 tgaagttgaa gacgatcatt ccgggcacga tcttcgctac tgtcggctgg atgctgttat
     5101 cacaagtgtt tagtatctat gctaggtatt ttgcaacgag gatcagcggt tatcagatca
     5161 taggaagttt tattgtactc atgctttggc ttaattttgc tgctacgatc atcattttgg
     5221 gcggaatcat caatgcagtt gttcaagaat acgtgacggg tcatcaaatc aaaaaacgtc
     5281 gagatccaag ccgaaaatgg ttcaataaat tgaaaactaa attttctcat aaaaaaaagt
     5341 agaaacaaga gttcttattg taaatttata tcagtaattt ataataatta tgactcttaa
     5401 ttaaagtagg tgcccatatg cttgtttctt ttagtatggt tataagattt tttctgacaa
     5461 ttttattatc actgatatta acacctgtat tcaaaatcat ttcagtacaa acaggtatgg
     5521 tcgacaaacc aaatgaaaga agaataaata aagttccaat gccttcagcg ggcggactgc
     5581 ctatctttat ttcatttgtt atatcgactt tattcttatt ccgtaaaatt atccctcaat
     5641 cgtatattgt accgatcttg attgcttctt tagtaatcat agtcacagga gtgctcgatg
     5701 ataaatatga gctgacacct aaacaaaaaa gtatagggat cttgattgct tctcttatta
     5761 tttattttgt agcagatata cgtattgatt ccttcacgct tccttttatt ggatatatcc
     5821 aactaggctg gcttagtttt cccgtaacga ttttttggat cttcggtatc acgaatgcgg
     5881 tcaatctgat tgacggtttg gatgggctgg cggcagggat ttcattgatc ggtttaatta
     5941 cgataggaat cattggatat tttttcctcc atgcttctac agtgtatatt ccagttgtta
     6001 ttttctgtct tgtagcaagt attatcggtt tttttcctta caatttttat ccggcaaaga
     6061 tttatttagg cgatacagga gctctattcc tagggtttat gatggccgtc ctttcactac
     6121 aagggctgaa gaacgtgact tttgtttcat cgatttcttt gctgatcgtc atgggagtgc
     6181 cggtaacaga cacgtttttt gctatcatta ggagaaaagc aaaccgtgtt tctttttcta
     6241 cagcagataa aaaacatctt catcaccgac taatcagttt aggctttact cataaaggtg
     6301 ctgtcctgac gatttatgcg atggctttga tgttttcttt cacagctatg gtcatgaatt
     6361 atactggaag aatcggaacg atcatattga ttattgccat gttgtttgct gcaattttat
     6421 tatttgaatt gattggtctg atcaatgaga aacatcagtt tattttaaaa acattgcgat
     6481 ttttagggaa taaagagtac cgtacgcaag taaaacagaa atatttgaag aaatggaacc
     6541 gtcatataga tgaataacaa attaaaccag ccgatcctta ttagtattgt gacggcaaac
     6601 agtgataaaa tttttcggac actagataat tttatttcaa ctgtaaaaga tgaaacagca
     6661 gtaaaaatcc gaatttttga caacaattca cttcccgagt acaaaaaaag actaacagag
     6721 tataggaaat atccctacat cgatatctat tttcatgatg aaaattccgg atttggttat
     6781 ggacataatc acaatctgat agctagcgat tctcgctatg gcattatttg taacccagat
     6841 attttagtgg atgaagagac agttacagca ttggtcttgt tattgaaaga acatccggaa
     6901 tgtgcgatgt tagcacctaa gattcttaat gaagatggaa caactcagca cctcatccgt
     6961 gagcagctgg ctgtttttga ttacgttttg cgctttgtgc ctttcaaatg ggtcaaaaaa
     7021 aagtttgaca aacgactggc tgtgtttgaa tgccgaggat tgcctactga tgctaatagt
     7081 tatgtacgga tgatctcagg tagttttatg gtagctgata ttgataaatt taaagagata
     7141 aaggggtttg atgagcgttt cttcatgtac tttgaagaca acgacttgtg tttgagtttt
     7201 gaaaaagcag gttacaagtt attgtacaca cccttttatg cagttatcca tttttacggg
     7261 aagggtgcgc atcgaaatgc aaaactgttt cgcgtgtttt tgacttctat gatgaaattt
     7321 ttcaataagt gggggtggcg ttttttctga tgaaaccaga aatattagta gtcgttgtgc
     7381 tttatcagca actattcagt caatctccag cttatggacc attaagcaaa gcacttagcg
     7441 aaaagcaaat tcagcttatt atttacgata atagtcctca aaggcagcaa aatgagctgt
     7501 ttgaaaaaga aaatatcgtt tatcatcatg atccgcaaaa tcctggttta gcggtggctt
     7561 ataattttgc gttgtctcaa gcagatgaag gaacacgttg tctcgtcact ctggatcagg
     7621 atacgaggct agtcgacgat tattttgaac tattgcgtaa agtaaccttt acagatgaat
     7681 gtgttgctgc agttccgatg attttttctg ggagccgaca agtttctccc ctatattcag
     7741 atcgttatat caatcgtaca gctcaagcag tagaagttga aggcccaaca gctgaacgaa
     7801 tcatggcaat caattcaggt gttgcctggt ctgtcaaatt tctaaaaaag attggcgggt
     7861 ttaatcctgc tttttcttta gattttttgg atcattggct gttttggaaa gtggatcagt
     7921 tggaaaaaac tgtggagatc cttcctgtac gaatggaaca tgatctttcg gttttagact
     7981 atgaaaaagt ttcaagtaat cgataccgtt ctattttaca agcagaaaat cgattttatc
     8041 atgaatatga tcaaaggcat ttatccagtc accgacgaca attattgtta agaacagcta
     8101 aacagttctt gaccgtcaaa aaccggcata tctggcggat gactttgcgc tcatatgtta
     8161 agaattggaa ggtgtgaatt cgtgcgttcg gtttgtatag ctacctttaa tggaggcaga
     8221 tatttaagag agcagttaga tagtatttta tctcagcttt cagcagaaga tgaagtagtc
     8281 atttctgatg atgggtcagt agatgataca tgggatatat taactgaata cgcagaaaaa
     8341 gataagcgaa tccagctttt ttcggggcct cgtcaaggat tgatcgctaa ttttggttat
     8401 gcgatccagc agacgaaagg agatctaatc tttttggcag accaagatga tgtttggttg
     8461 gagacaaaag tgaaaaagat cactgactat tttgagcaga atccggatca gctagtagta
     8521 gtttctgatt taatcattgt ggacagcgag ctgcaagaga tccatgattc gtatttttcg
     8581 tttcgaaatt ccaaagaagg atggcttaat aatgtgataa gaagtcacta tattggtgct
     8641 ggtatggctt tccggtcttc tttaaaaccc gatattttgc cgatcccgtc agaagtgccg
     8701 atgcatgata tgtggattgg tttgctggga ggaaagaaga ctgggttcct aagagaaccg
     8761 ttaactctgt accgaagaca tgatttaaac gcgagtgaga ttgaaacaca ttctaaatta
     8821 cggcaaaaaa tttcttggcg tgttcatctg attacggcgt tgatcaaacg aaaatggttt
     8881 aaacgttgat aaaagatgtt taaatcctta atatttccat gacaaatata acaattgatg
     8941 agataataac aaagtgaaat ttgaaatcaa aaaggagagc agatatccat gaagggaatc
     9001 attcttgctg ggggcagcgg aacacgtctg taccctttaa caaaggcaac atcgaaacaa
     9061 ttgatgccga tttatgacaa accaatgatt tattatccaa tgtctactct gatgttggcg
     9121 ggaatcaaag aaattctgat tatttcaacg ccacaagata caccacgttt caaggaatta
     9181 tttggaaacg gaaatgattt aggactgcag atcgaatatg cagtacaaga aagtccagat
     9241 ggattggcac aagcgtttat cattggagaa gaatttattg gagatgatag tgtctgcttg
     9301 gtattagggg acaatattta ctatggtggc ggtctgtcta aaatgttgca acgagcagct
     9361 tcaaaagata caggcgcgac agtatttggc tatcatgtca atgatccaga acgctttggt
     9421 gtagtagaat ttgatgagaa tatgcaagct ttgtcgattg aagaaaagcc tgctcagcca
     9481 aaatcaaatt acgctgtaac tggcctttat ttttatgaca atgaggtaat ttctatcgct
     9541 aaaggtatca aaccttcaga acgtggagaa ctagagatta cggatgtcaa taaagcatat
     9601 ttggataaag gaaaactatc cgtagaactt atgggacgcg gatttgcttg gcttgataca
     9661 ggaacacatg aatcattgct tgaagcatct acctttatcg aaacaattga aaaacgtcag
     9721 aacttaaaag tggcttgttt ggaagaaatt gcttaccgta tgggctacat cgataaggaa
     9781 caattgctgg cccttgcaca gccattgaaa aaaaatcaat acggtcaata tttattgaat
     9841 ttggccgctg aataaggagt ccttacagat gaaagtaaca gatacaaaat taacagatgt
     9901 caaaatcgta gaaatggacg tctttggcga tcatcgcggt ttcttcacag aaagttattc
     9961 aaaagcaaaa tttgcagaac atggattgaa atatgacttt gttcaagaca accattcact
    10021 atcagctgaa gctggtgtcc ttcgggggct tcatttccaa aaaggagaag ctgcacagac
    10081 aaaattgatt cgggtagtca ctggagcagt tttagatgta gtagtggata tccgcaaagg
    10141 tagcccgact tatggtcaat gggacggatt tatcttatct gaacataatc atcgacagtt
    10201 gcttgtacct aaaggatatg cccatggatt cgttactttg acaccaaatg tgaacttcct
    10261 ttataagtgt gataattact ataatgcaga agcagatggg ggcatcgcat ttgatgatcc
    10321 agatttagcg attgattggc caattgatcc atcaagagcg attatgtcgg aaaaagatca
    10381 ccgtcatccg acattaaaag aatttgaagc agaaaatcca tttgtttacg gagaaatcta
    10441 agaattaagg agaaattttc ttatgaaaaa catcattgtt accggaggtg ccggattcat
    10501 cggctctaat ttcgttcatt acgtggtaaa caatcatcca gaagtgcatg tcactgtttt
    10561 ggataaactg acttatgcag gaaacaaaga gaacttggcc ggacttccta gcgatcgcgt
    10621 agaattggtc gtaggagatg ttgcagatgc agaattagtg gaccgtctag taaaagaagc
    10681 ggacgcagtt gttcattacg cggcagaatc ccataacgat aattcattaa aagatccttt
    10741 cccatttgta caaacgaata tcattggtac gtatacactt ttagaagctt gccgcaagta
    10801 tgatgtacgc tatcatcacg tatctactga cgaggtttac ggcgatcttc cattacgtga
    10861 agacctgcca ggacatgggg aaggcgaagg agaaaaattc actgcggaaa caccatataa
    10921 tccttcaagt ccatattctt ctacaaaagc tggttcagat ctgttagtaa aagcatgggt
    10981 gcgttctttt ggcttacgtg caaccatttc aaactgttca aacaactatg gtccttatca
    11041 gcatattgaa aaatttattc ctcgccaagt aacgagtatc ttaagcggta ttcgtccaaa
    11101 attatatggc gaaggaaaga atgttcgtga ctggatccat acgaatgatc attcttcagc
    11161 agtgtggctg attttgacga aaggcagaat tggcgaaacc tatctgatcg gtgcagacgg
    11221 tgaagaagat aataagaccg tgatggaatt gatcttagaa atgatgggcc aaccaaaaga
    11281 cgcatacgac catgtaaacg accgcgcagg gcacgatttg cgttatgcaa tcgattcgac
    11341 aaaattgcga gaagagctag gttggcagcc tgagtttacg aacttccgtg atgggttggc
    11401 tgacacgatc aaatggtacg aagaacatga agactggtgg aaaaaagaaa aagaggccgt
    11461 tgaagcagca tatgccaaaa atggacaata atacagagga aatttcactg tattaatgga
    11521 aaaacacatg tgaggcaggc gctcgcctgt ctctttttta aaataaaaat caaggagcgg
    11581 ataaccatat gtttttatta actggaggaa atggacaatt aggtacagaa ttgcgccatt
    11641 tattagatga aaaaaatctg ccatatgttt caacagatgc aaaagacttg gatatcacag
    11701 atgctgagaa aacaatggct tatattacag aattaaaacc cgatgttatt ttccattgcg
    11761 cagcttacac agcagtagat aaagcagaag aagaagcaaa agaattagat gagaaaatca
    11821 atgtcgatgg cacaagaaat gtagcacttg cagcaaaagc tgtgaatgca caattcgtct
    11881 atatcagtac agactatgtt ttcgatggaa aaaacaaaaa tgtagaatac gaagtagacg
    11941 accaaacgaa tccactaaat gaatatggtc gtacgaaatt attaggcgaa aaagcagtag
    12001 aagagatttt agaagactat tatatcatac gaacttcttg ggttttcggg atctatggac
    12061 acaatttcgt cttcacgatg caacgcctag cggaaacccg cgacaaactg actgtagtaa
    12121 atgaccagtt tgggcgacca acttggacac gtactttggc agaatttatg gtatatgtca
    12181 tcgaagaaaa agcaccattt ggtatttatc atttgtcgaa cgaaaatagc tgttcttggt
    12241 atgagttcgc aaaagaaata ttgaaagata cagatgtaga agttgcacct gttacgtcag
    12301 aagaatttcc gcaaaaagca acacgtccgc agtattcagt catgagcctg aaaaagacag
    12361 aagcgttagg ttttgtcata ccaacttggc aagaagcact tgcgcaaatg ctggaagctg
    12421 ccaaaaaaat gaataagtaa tttccttttt atcaaataga atttggagca tgatatggaa
    12481 aaacaaacaa gaagtgcacg acgaaaagaa aagccaaaag cgaatcaggc agattcgaat
    12541 ttggtgcttg acctgtttct aaaactccgc tatcttattg gcgttttagt catcgtggtc
    12601 atcgttactt ttaatttaaa cggaagttca cttggtgttt gggataaata tgtgtcgcaa
    12661 agagacgatg ggaaaaaaac tgatgtgatt tttggacaga atcgggaagt gcggtcagac
    12721 gaatggctgg tgcagacacc tttttacctc tcacaagctg aagaggactt ttcgttggtg
    12781 aacgaggatt attcattaaa cggacaaaac atgatcattg cgtataattc tcctgtaaag
    12841 gatattacgg ttatcggaaa accattcaac tggggattct ttttcctcgg aagagatcga
    12901 ggactttcct tttattgggc gatgaaatta gtcgtcatgg tcttgcttgg attcgaagta
    12961 ctgatgatat taacgaagag gaaaaaagct ctatcgctaa tcggtgcatt tgcgctgaca
    13021 ttttctccag cagtccagtg gtggttcatg caacatgtgg gcgatttgat tttctttact
    13081 ttgggactga tgattgcttt ttatcattat ttctacaatc atgataaaaa atggctacga
    13141 acgttgatga tgcttttggt ggttattttc ggtattgggt ttattttagt gatttaccca
    13201 gcccatcaag tgatgctggc gtacttgctg attttttatt ttgttggcct gcttatttac
    13261 tatagcaaga aagtttcatg ggatctgttg gatgtagtgt tgattggagg ggcagtttta
    13321 tttattggcg gagtcatggt tcatttctgg ctaacgtcaa aagacgcatt gttagcttct
    13381 ttgaatacct tatatccagg aaaacgggta agtactggag gaaagtggac gattggcgaa
    13441 tttttttctt tccttacaaa ttggaaaatt ccttttgaag atatcaacta ttcgaataac
    13501 tctgaagtgg ccctgttcta tcactttttt ccaactgtat tcctagcaag tccatttgtg
    13561 ttatttggca aaaaagatag cgaacaaaag ttactgggac gtattttgat gattttttgt
    13621 ctgttttcta tcttttggat taccgttggg ttgccaaaat ggctagcaga aatcactctg
    13681 ctgtcttatg ttcctccatt aagagccatc cttactttta gttttgcggc ttgtttatta
    13741 tcgatctggt ttatcgctta tatttggaga gaagatgtta ttcctatttg gtataaattc
    13801 attttgttgg cagtaaatgc atggtcttat ttctatgcga ttacgcattc gaaaatggat
    13861 aattattttt cggattttga aaccatcgca gttgctgtgt tgggaacagt gattttagct
    13921 tttgcattat ttggctggag acgcttgttt tacctcgctt ttgtcgcctt agttatcgta
    13981 tctggctttt tcgtcaatcc agtcgtagaa ggtacaggag cgatttttga aaaaacactg
    14041 gcaaaagaaa tccaaaaaat cgatcaacaa gatcctggta aagtttggtt gtctgaagat
    14101 gaattatata acttcacccc aacattggga gtgaaatcct tcaatacagt acgcttttat
    14161 ccagatatag ctgcatggaa gaaaatcgat ccgaaagaag aatacgagaa gtattataat
    14221 cggtatgctc atacaagagc ttttatcgct aaggaagata caaagttcat tttagatcgt
    14281 ccagatatgt tttcggtcac gttgaatttt gaagacgcag aaaaattagg gatcgactat
    14341 gtgatcagta aacgaccatt aactgattac aaccaattat atgaagcgca gttcacgcag
    14401 ctatatggac cggataaaga cggttatatg atttttaaag tagaatatcc ggattatcca
    14461 gatgtaccac aaacacaaac tcaggaaaat cagctagtcg tacagtaata ggctagctga
    14521 gaataatcct gaaatattag tagaaaggag agtgaagata attcaagcaa tcgtacaaaa
    14581 atttgtcaac aaaatgagtg tcggtttctt gcttttgttg ctgacgctag tcttttttgt
    14641 ttggacagca ggggtaggtt ttccagcaac tttgtttata tataacaata gttcattact
    14701 tatcgctttt agtgtggcta ttttgctcat tttaaatgtt tataaagcaa aaggcgtcga
    14761 ttttctttat ctaggtgttg cgcttgccct tttcattttc ttttcttact ttcgggaatt
    14821 aagaaatgag tctttatttg gagacttact gtttcctttg attattttat tttgtttagt
    14881 aattaaatgg atcgagtgga caaaatggga tcgcggtatt tatctgattg tctttacggt
    14941 cttcctcgga atcacgttat atcgtgtatt tacagagatc caagtaccag aaggacaaag
    15001 catctgggct ccaggcaatt cgctttctga tatttggatc aatacgaata caatcggaag
    15061 ttctttgatg actcttgctt tattgatcag tggatttgcc agttcttttg aacgatggta
    15121 tgttcgaatc atcggtggga tagcagtcgt tgcagccttt ttaggtattt gggtctgcca
    15181 atctagaggt gcattagtcg cgttgattgt ttttgcgata ttggatcttt tacctaaatc
    15241 gttgatgcgt gtcattcgtg cgccatttat tgcctacacg gttacgattc ttttagcttt
    15301 gccgatttct tatctggcgg ctgtctctga aaaagtcaat ttgtttaccg gtcgagaaga
    15361 tatttggcat aaattctatc agacgctagg agaaaaatct gaacaagttt tactaggaat
    15421 gaaaacattt atcttccagc gaggaaacca atttttagga aaccacaata gttataattc
    15481 tattttgaat atctatggat tgataggatt tgggattgct gctttgctgc ttatcctttt
    15541 tattggtcgc ttaacgttga aagcagattt atccaacggg cagatgactt ttatctgggc
    15601 cttttttgcg gtgatgatgc agtcatttat ggaggataca ttgacttcat accgatgggt
    15661 tccgattatt tatatcctgt tggctatggc cgttcatcga tatgatgaac cagaagttac
    15721 ggaggaattc tatgttatgg aagaggcata tgacgatatg acttatgatg atacatctta
    15781 tgaggaacaa ccagatagcg ctcattcttc caggatccaa cgctatcatt gataggcaca
    15841 aaatacgaga gaggctgtga caaccgtcgc agcctttttt ggtttattgc aattgtctag
    15901 catcttctgt aaaatagaaa cgttagagaa aatcttgtat catagaaaat taagaaagaa
    15961 ataagaacgc agctcggtac caaaaatatg gtaaaatggc acttgactgt aattcactga
    16021 aaatcgcttg gaggaacaca aatgtttaaa gatttgtatt tatttatgaa ggacatttat
    16081 cagaacagaa aattactgct acagttttcg atcaatgact tcaaatcaag atatgcagga
    16141 tcgtttttag gaatcttatg ggcgtttatc aatccgctat ttactgtatt gatttattgg
    16201 ctggtatttg gtgttggact gaaagcacaa atgatcgatg caaaataccc atttatcgta
    16261 tatttagtta ccgggatgat tccctggttc ttcttctcag atgcatttgc atcaactacg
    16321 gttgtttttc gggaatatac ttatttagta aagaaagtcg tattcaatat tcgtatcttg
    16381 cccacgacga aaatcctatc caatctgtac acccatttat tcttcattct gattggcata
    16441 gctgtcgcgc ttataaatgg tatatatcct tcagcaatgt ctttacagct gatttattat
    16501 ttattttgca tgtgtgcttt cttgacagga ttgacttggc tgacggcatc gatccaacca
    16561 tttttacctg atatcatgca attaattacg atcgtgctgc agttgatcat gtggacatta
    16621 ccgatattat ggagccctaa tcagtttgat tcaggtcttg taagcatatt gaaattaaat
    16681 ccgctgtact atgttgttca aggttatcgt gagtcatttt taagtgaagc ttggttctgg
    16741 gagcatggaa catatacttt gtatttctgg gttttcacgt tagtaatgtt cgtaatcggc
    16801 tctgcagtgt tccgtagatt gaaaccacat ttttcagatg tgctgtaagg agaatagcta
    16861 tgacagaata tgcaattaga ctaaaagata tcacaaaatc ttataatatg tacgcaaaac
    16921 cttcggatcg atttcgtgaa gccctcaatc cttttaagaa atcctatcat gatgtatttt
    16981 atgcgttgaa aaacgtgaat gtagaaataa aaaaaggtga gatgatcggt ttcgtgggag
    17041 aaaacggatc gggaaaatct acaatgttga aaatcattac gggagtactg acaccaacat
    17101 ccggcacgat ggaaatcgaa ggaaaaatct cagctttgct agaactagga tcaggattca
    17161 atcctgagta ttctggctat gaaaatatct atttaaatgg aatggttcta ggattttcaa
    17221 gagaagaagt cgatgaaatg gtcgatgata tcattgaatt tgctgacatc ggcgaccata
    17281 tttatcaacc tgtaaaaacc tattcaagtg ggatgttcgt tcgtctagct tttgctgtag
    17341 cgatcaatgt gaatcctgat attttgattg tggatgaagc attggcagta ggagatcttg
    17401 aatttcaact aaaatgtatg gaaaaattta cagaaatcaa gaactcggga aagacgattt
    17461 tattcgtttc tcatgatatt agttcgattc gccgtttttg tgatcgttcc tattggttga
    17521 aaaatggaaa agttgtcgaa tatggtgata cgatggaagt aacgacgaat tacgaaaact
    17581 ttttgaagaa aaaatcagtt aaaacagtag atcgaaataa aaaccaagta gaacgcttcg
    17641 cagctccaga tatcgtagac gtgacgaaag ccgaattgtt ggataaagat aaaaatccag
    17701 tggatatgct ggaacaagga gaaaaactat acgttaaagt aacctatgaa gtaaaagacg
    17761 atacaatcaa aaatccagtt ttaggaattg ctctgcgtac agtcgacaat ttttatgtgt
    17821 gcggactcaa tactctacta gatggtatca agatcccttg gaaaaaaggt gagaatatct
    17881 tttatctgga gtatgaaaag ttaggtttgt tagctggtga atattatttt gacgttgcgg
    17941 tctttgaaga aaatgcaacg gttcctttgg tatacaagac aaaatacatg aacttgtttg
    18001 tcagcggttc ttatatcgga gaaggcatcg tggtcttaga ccataaatgg gaagaaggga
    18061 ctcatagcaa tgaaatatga ttttgaaatg gacctagatg aacaaagcag tgtgggaaaa
    18121 attgccgcac agatcaaacc aggaagtaaa gttttagagt ttggtcctgg aaacggccga
    18181 ttaacgaaac atttgatcgg agcaaaacaa tgtgaagtga gcattgtcga gctagataag
    18241 gaattgttcg attttgtcag cgagtttgcc caagatggat tttatggaga tatcgaaagt
    18301 tttgaatggg caaactatta tgctggacaa acatttgatt acgttttgtt tgccgatgtt
    18361 ttagaacatt tggtagatcc tggaaaaacg ttgaagaaag ttagagaatt tttgaatgaa
    18421 gaaggagaaa ttctaattac attccctaac cttgcgcata attctgtcat gattgatttg
    18481 ttcaataatc aattgccatg ggcttcttat ggtttgctag atgaaaccca taattctttt
    18541 tatactcatg atggcttcca aaaagttttt gaaaaagcag gattatacat caatattgaa
    18601 gattatttgt acttagctgt aggtgataca gaattaaaat caacttatga agaattgccg
    18661 gaagctgtac gttatgattt caaaatgcga ccatttggcg aagtctacca atacttcttc
    18721 tcattgatga aacatccagt agcacaaagt tccattgcgc agccgcaaaa ttctaattat
    18781 gtgaaagtat tagaagtaat ccagcaaacg aaacaagatg aaataattca acaaatacca
    18841 ttcaataatt tcacagggga aaatgagaca ctaagtttcc atgtatcaga attagcagaa
    18901 cgaatggtgt ttcgcttcgc gcagcaacct agctttatcg aattcagcgc agaagctgct
    18961 ggagaaaaac tcacatttat cgattcaaat gctgtagtca aaacagtaaa tgattgttat
    19021 ttgtttgatg gtaaagattt accagagttc tttttaacgg atattgcagg gaaagaagtg
    19081 actatccatt gtcactatcg ttttatcgga gaattgacac caacaatgaa agaattgctg
    19141 gaaacaatcc gtccaatggc tgaagttaca aagcagcttt ctgagaaaaa tgatgaatta
    19201 gaaagagaaa atcattatct tattgagaat aatacaagat tagatcagac gttgaaaaca
    19261 accataaatc gctattgcac gttgttggaa agtgatgaat ggacaatcaa acatagatta
    19321 gggcgacgta agaaagaaac atcgaaaaaa atccaagaaa aagagctttc tctatgtatt
    19381 gacgaaaaaa tctgggatgc agaaacaaag atattgaaaa tcatcgggtg gggaatcgct
    19441 aattcggatc gccaaccatt gtcctataag ttatcagccg accaatcacc attttttgaa
    19501 gcaattcctg ttttacgaga agaagtcaat caagcaaaac agctagctga aggagcaaaa
    19561 gcaggatttg aactgcggat cctttgcgag aaggaacgtt cattcttagt tgaagcagtg
    19621 gctcaaaatg gtcaatcatg gatcattgaa atgtaaataa tgattaactt ataaagataa
    19681 ggagtaaacg aatcgtgcaa aaaaaagaaa ttgccgtatt tatcgatcaa atcactcgtg
    19741 aacgggccac tggcgatttg cttattgtgg gctgggcaat tgatgaagtg accaaagaaa
    19801 tccctacgat aaaggtagaa aaagaaaatg tgattgctga agcaacacat gtcgtaagat
    19861 tggatatcaa tcatttatat aatcttgatg ttaagaccca aagcggtttt aaggtacgtt
    19921 tatcagggaa aatgagagga aaagcaatct tagacttcca aacagcaaga catcaaaatg
    19981 gcatagctgt gaaattaaac ggtaaatatc cttatgatga cggcattgaa tcttcatggg
    20041 aaagaaaaaa aagattactg aaaaaaggga taaattatgc aaggactcat ggcgtaaaaa
    20101 aagcaatccg ccgggtcaaa ctggaattga aaccaggatc gatcgactat gcagagtgga
    20161 tcagtcgtca tgagaaacct gatctgaaag agcaaaggaa agaagtacaa ggatttgcct
    20221 accgcccatt gatttccata cttatgcctg tctataacgt tgaaatcaaa tggctcgaaa
    20281 aatgcattga ttcggtactt gatcaaacgt atgatcactg ggaactatgt atcagtgatg
    20341 atgcttctac tgatccagct atccgaaagt gtctagaatc ctatcaagca aaagatgata
    20401 gaattaaagt cgttttccga caagaaaatg gacacatcag tctggcaact aattctgcat
    20461 tggaaatggc agaaggagag tttatagcgc tcttagataa tgatgacgaa ttacctccat
    20521 ttgccttata tgaagtggca aaagtgctaa acgtacatcc agaactggat ttgatctaca
    20581 gtgacgaaga taaaatcgat gcagatggga atcgctttga cccgcacttc aaagcagact
    20641 ggtctcccga tacattgatg ggaaataatt atatctctca cttaggcgta tatcggacaa
    20701 gcattgttaa ggaacttggc ggtttccgta aaggatatga aggctctcaa gattatgatt
    20761 tggttcttcg tgtgacagaa caaattcctg cagaacacat ttaccacatt gaccgtgtct
    20821 tgtatcattg gcggacgatc ccgggatcaa ctgcaagcaa tggagaagct aaaagctaca
    20881 tctatgattc tggagtgaaa gcattgacag atgctctatc tagaagaaat atcaagggaa
    20941 gcgtacatcc tggcagaatt tctggttttt atgaaatcac ttatgatgtt ttgcaagaag
    21001 acttggtcag tgtgatcatt ccaactaaga atggctatga agatctaaaa acttgtgtgg
    21061 attcgatcat cgaaaaaacg tcttatccaa attatgaaat cattattgca gataatggaa
    21121 gtacagatcc taaaatgcaa gaattatttg cagaatacaa gcatcagctg aaagaccgat
    21181 ttatcgtcga attaattgac attccattca attattcacg tatcaacaat ctggcagcag
    21241 aaaaagcaaa cggcaaatac tttttattct tgaataacga tacagaagtg attgaacctg
    21301 actggatgac agcgatggtt tcttatgcac agtttgatcg aatcggctgt gtaggagcaa
    21361 aattatttta tccggatgat acaacgcagc atgcaggtgt attattaggg atcggtggcg
    21421 ttgcaggaca tgcattgaat aactatgatc gaacacattg tggttacttt ggccgattgg
    21481 tcattgacgt caattactta gcagtcactg cagcttgtat gatggtcaag gcagctgact
    21541 tcaatgctgt caatggattt gatgaaatat tagaagtagc attcaacgat gttgacttgt
    21601 gcttgaaagt ttatgaactg ggtcgttaca atgtctatgc acatcaagca gaattgtacc
    21661 atttcgaatc taaatcaaga ggttatgaag atacaccaga aaaacaaaga cgttttgctg
    21721 gggaaatcaa aaaaatgcaa gataagtggc cttcttatat tgctcatgac ccattttata
    21781 acgataatct aactaaagaa ggaatcggcg atttttcgtt gagacctgat tgattaaaag
    21841 aactgtattt tcatttttat taatgaaaat acagttcttt taatttaatt
//
LOCUS       DAZKLD010000038        21714 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.038, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000038 DAZKLD010000000
VERSION     DAZKLD010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21714
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.038"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            121..1125
                     /locus_tag="ACG8KR_001156"
     CDS             121..1125
                     /locus_tag="ACG8KR_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0200539.1"
                     /translation="MSDNQLLEVQNLHTGFRLKDDYYDAVDDVSFTLDKNEILAIVGE
                     SGCGKSTLATTIMGLLDPNNTKITGEILYNDLNLIDLNETLYNKIRGNDIGMIFQDPL
                     SALNPLMRIEDQIKEGLSYHTKMNAEQRQARALELLEQVGIPNPARVGRQYPHELSGG
                     MRQRVIIAIAIACKPPIVIADEPTTALDVTIQAQILDLLKDLQEETKTGIILITHDLG
                     VVAEMADRVAVMYGGQFVEVAGVKELFDHPKHPYTRSLLNSIPQEGNHDAELHVIEGV
                     VPSLKNMPRKGCRFAPRIPWIPASDHEEEPILHEVAPNHLVRCSCYKHFHFRDEKGEV
                     "
     gene            1126..2070
                     /locus_tag="ACG8KR_001157"
     CDS             1126..2070
                     /locus_tag="ACG8KR_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0200540.1"
                     /translation="MTELITIKDLKVHYPIRSGFFNRVTDHVYAVDGVDFIIEKGKTY
                     GLVGESGSGKSTTGKAVVGLEKVTAGQIMYEGKDVTKRSNRKKMGYNKDVQMIFQDSM
                     SSLNPKKRVLDIIAEPIRNFERLSDQEEKKKVKGLLDIVGMPEDALYKYPHEFSGGQR
                     QRLGVARAVATNPKLIVADEPVSALDLSVQAQVLNFMKRIQQEYGLSYLFISHDLGVV
                     KHMCDNIAIMYKGRFVEIGTREDIYNDPRHIYTKRLLSAIPQIDVENRESHKENRRRV
                     EQEYIQNQKEYYDATGRVYDLRTITTTHKVALKDGGAS"
     gene            2070..3032
                     /gene="opp4B"
                     /locus_tag="ACG8KR_001158"
     CDS             2070..3032
                     /gene="opp4B"
                     /locus_tag="ACG8KR_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease"
                     /protein_id="HFQ0200541.1"
                     /translation="MWKTILRRVLLMIPQVIILSVLIFLLAQAMPGDPFTGLINPNQD
                     PAVIEQMRQAAGLNDPWYVQYFRWIGNALQGDFGQSFLYKRSVADLIGERIINTLYLS
                     ILTVVLTYLIAVPLGMLAGRYQNSILDKIVVIYNFFSFAVPLFIFGLIMLFVFGYRLG
                     WFPTSGSQTLGLQGGFITQWIDRLKYILLPSITQAFLATAVTIQYLRSEVIDSKSLDF
                     VRTARSKGVPTNKVFSRHIFRNAALPMASQMGYEITSLIAGSVVIEKIFGYPGVGKLF
                     IDSIGQRDYTVITSLVLILGIATLVGTLLSDIIMSIVDPRIRIQ"
     gene            3059..3973
                     /locus_tag="ACG8KR_001159"
     CDS             3059..3973
                     /locus_tag="ACG8KR_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0200542.1"
                     /translation="MSEKNNTAVQESIPPMGIRMIAREFKKDKIAIFSLGLLVVLLLV
                     IFIGAMLTDQDKVMYVSIFDKYAAPGTVSTQGQKFILGADEGGRDVLGQLIIGARNSV
                     TIGFAITILTSIIGVGLGIISGYYGGIIDNILMRIVDFIMILPIMLIIIVFVSIISRY
                     NVWSFIFIMSAFYWVAKARLFRSKTLSEARRDYVSASKTMGTNDFKIMFREIMPNLSS
                     LIITNLTMNFAANIGIETTLTFLGFGLPANVPSLGTLIGYASNGEVLVNRQWIWLPAS
                     ILILVLMLSINYVGQAFKRSADARQRLG"
     gene            3999..5780
                     /locus_tag="ACG8KR_001160"
     CDS             3999..5780
                     /locus_tag="ACG8KR_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HFQ0200543.1"
                     /translation="MKKTIFGFAALMSAVVLAACGGGGTDTSGNNSGGNGNNKEELTF
                     PIATKNNDDAIEDGELEAAVATDSQFKGLFQWEFYQDSYDAAFMAPSHEPLFANDDDF
                     RITDDGAATLDLDEDTKKATIKVKDNVKWSDGEDVTADDVIFPYEIIGNKDYTGIRYD
                     DTFRNIVGMEEYHDGSADTISGIKKIDDKTVEIEYKEVNPSMLQSGGGIWSYAAPKHT
                     LEGVAIKDMESSDQVRKNPVTFGPYYMSNIVAGESVEYLPNEHYWNGTPKLKKITMKS
                     VPMASATEALNSKLYDMIFQMPTDTYDTYKEIAGYTNLGREQTSYTYLGFKLGKWNAD
                     TKSVEYNPDSKMADKSLRQAMGYALDNNAVGERFYAGLRSNATSLIPPVFQSYHDDEM
                     KGFTQDLDKAKKLLDDAGYKDVDDDGLREDKDGNKLTINFASMSGGETAQPLADYYIQ
                     QWKEIGLDVQYTTGRLIEFNSFYDKLENDDPEIDVYQAAWNTGTDPNPSGLWGPNAAF
                     NYTRYESEENTKLINDISSSKAFDEEYQKEAYQKWQQYAFEEAFAIPTLFRNEVLPVN
                     DRVKDWDWSYDVPNPWANVSVTADSRS"
     gene            6128..6814
                     /gene="rnc"
                     /locus_tag="ACG8KR_001161"
     CDS             6128..6814
                     /gene="rnc"
                     /locus_tag="ACG8KR_001161"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HFQ0200544.1"
                     /translation="MDNQLTKELKENYNIVFHDLNLLEQAFTHSSYVNEHRNLQLSDN
                     ERLEFLGDAVLELMVSQYLFRLYPHLPEGKLTKTRAAIVREDSLSKFAKECHFDQYVL
                     LGKGEENSGGRTRPALLCDLFEAFLGALYLDQGFDTTHAFIEKVIFPKVKAGAFSHEM
                     DHKTKLQEVLQKSGDVSIEYRLINEEGPAHERVFWIEVYVDDQLIGTGQGKSKKLAEQ
                     AAAENALAAL"
     gene            6834..10415
                     /gene="smc"
                     /locus_tag="ACG8KR_001162"
     CDS             6834..10415
                     /gene="smc"
                     /locus_tag="ACG8KR_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HFQ0200545.1"
                     /translation="MYLKRIEIAGFKSFADKTVIDFENSVTAVVGPNGSGKSNITEAI
                     RWVLGEQSAKSLRGGKMPDIIFAGSDTRKQLNIAEVTVILDNSDHYLPLEYNEISVTR
                     RYRRTGESEFFINKQSCRLKDIQELFLDSGLGKESFSIISQGKVEAIFSSKPEDRRGI
                     FEEAAGVLKYKQRKKKAEQKLFETEDNLSRVQDIIHELEEQLTPLAAQSEAAKEFLRL
                     KETLTQTDVSLMVAEIKTAKKDWDNKQAQLAKFNLELGKLSESIQEQESILAKQRKEN
                     AQADRLIEKNQQVLLDLSEKLKQTEGQKDVLQERTKHTQKSSQEYQTSLAEAQKKVKH
                     FEKLQESLMKAAAEKETEIQKAEANLIKTQQELEKYQKSTKELLAELRDQYVDLMQEQ
                     AAVGNELKYLERQYIQETAKSKQTLAKQSEVEASVDRLILQKEELTQKQAQLKSSLTE
                     TKEKLEMIQQNGKKFQEALAKEQPKMYQLMNQVQQLRARKKSLQEIQENYFGFYQGVR
                     LVLQHKQQLSGIVGAVAELIDVPADFTLAIEAALGGAAQHVIVENEKDARQAITYLKQ
                     QRGGRATFLPLTTIKPRQLPAHILTQAAAVEGFIGIASEQVSYPDQIQTVVQNLLGTI
                     LLAKDLTSANAIAQTIRYQYRVVSLEGDVMNAGGSMTGGANKRGNQGSLFVQNQELKQ
                     LTSEFEEADKQLQAQEKKVQELQQETARLAEEQEVLRTRGEQLRFEEQEATNQLQNII
                     NELERFEKEKQISTFETRELQQFIEDYEKQTNELKDKQTDLESQRQKIDEEIKSLSQE
                     SDQMEARRAQVQSQKAQEQADLAVLKEQFNHLQIQLRGARVQKAEATERQEAIEKQLA
                     TLTADFSDHEVTEESLEKQINELSAQRETLKAELAKAKEQRDRRQKEIDKLEAVLAER
                     NREQKARLSEQSKLEVQKDRAEMILDNHLTYLQSEYQLTFEKASQDYQETTDIEDSRT
                     KVSSLKEQIEKLGPVNLNSIEQYEQVSERHTFLATQRDDLLAAKNQLFETMDEMDDEV
                     RTRFKEVFEAIRQEFKIVFPNMFGGGKAELVLTEPTDLLKTGIEIEVQPPGKKLQSLS
                     LLSGGERALTAIALLFSIIRVCPVPFCILDEVEAALDEANVKRFGRYLSDFQDDTQFI
                     VVTHRKGTMVAADVLYGVTMQESGVSKIVSVRMEDINEEAKINETKV"
     gene            10424..11233
                     /locus_tag="ACG8KR_001163"
     CDS             10424..11233
                     /locus_tag="ACG8KR_001163"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFQ0200546.1"
                     /translation="MIKLVAIDLDGTLLNEQKLISDENKQALAQAKQQGVKIVLCTGR
                     PLAAMAHYLQELGLVDEGDYSITFNGGLVQKNDTGEIMEKKVMEVSDIQRLYKLAQQL
                     DLPLDVLSDNVVLQLPSAPRKQSLYNVLNKLLQFQPADISDITDEFVLNKAVIAYDQE
                     ELDPKIKEIPAEFHEMYEIIKTRSMLLEFMPKGVTKAYGISLLAKDLGLKQEEIMAIG
                     DEENDLPMIQYAGLGVAMENAVPFVKEAADFVTASNEENGVAKVIQKFVLE"
     gene            11244..12242
                     /gene="ftsY"
                     /locus_tag="ACG8KR_001164"
     CDS             11244..12242
                     /gene="ftsY"
                     /locus_tag="ACG8KR_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HFQ0200547.1"
                     /translation="MGFFDKIKRAFTGEEKTEEVEVQEKYDKGLEKTRKTFGERMNEL
                     FANFRTVDEDFFEELEETLIGADVGFETAIRITEALRQEVKLRNAKKSAEVQNAIIEK
                     LVDLYEAEGINEVNELNIQEDGLTVILFVGVNGVGKTTSIGKLAHQFKNEGKKVLMAA
                     ADTFRAGAIDQLVVWGERAGVEVVRGNAGGDPAAVVFDALERAKTEQADVLLVDTAGR
                     LQNKVNLMNELEKIKRVIQRELPEAPQETLLVLDATTGQNAMVQAKQFKETTDVTGLV
                     LTKLDGTAKGGIVLAIRNELHLPVKLVGLGEGIDDLEPFDPNDFVVGLFKGLLKEE"
     gene            12429..14696
                     /gene="gshAB"
                     /locus_tag="ACG8KR_001165"
     CDS             12429..14696
                     /gene="gshAB"
                     /locus_tag="ACG8KR_001165"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HFQ0200548.1"
                     /translation="MMNFKQLLLHVNARPFIDQARFGIEREGQRVDLAGNLAKTDHPA
                     IFGDRSYHPYIQTDFSETQTEMITPVTDSIPELFQYLAAVYDVTARSIPKEEMIWPLS
                     MPPALPEKDEEIIIAKLKNFEDVLYRRYLAKKYGKRKQMVSGIHFNFEFGDELLRTLF
                     SHQEEFQDFSEFKTELYLKTARNFMRYRWMITYLFGASPMSEKNYFLDESHPQEPVRS
                     IRNSALGYTNHPNVKVSYASMKQYLADIERMIEEGKLSEEKEFYTPLRFRGGKKVADL
                     ATTGVRYIELRNIDLNPYARLGINPEQVRFLQLFLMYMLWTEEKEDCDQWVAEGTTRN
                     NKVALEQPSDQTEFHQEGREILEGMKQMLVELDWLDSLYLVEEALTQMDHPEQTLAAK
                     LYQEAQLSSQQEVAVALGHQYYKESHERPYQLAGFREMELSTQIFMFDAIQKGVQVKV
                     LDESDQFLRLQFQDHVEYVKNANMTSKDSYIVPLIMENKTVTKKVLKEAGFRVPGGAE
                     FSSMEEAVKAYPRFAEQAFVIKPKSTNYGLGITIFKEGASLEDYQAGLAIAFREDSSV
                     LVEEFMPGTEYRFFVIDGEVQAIMLRVPANVIGDSIRTVKELVEEKNSDPLRGTNHRA
                     PLELIQLGELEQLMLKEQGLTIESVPQANQIVYLRENSNISTGGDSIDMTDEFSEAYK
                     KIAVSAVEALGAKISGIDLIIPDKEIDPTTDKKAYGIIEANFNPAMHMHVYPFAGKGR
                     RLTMNVLKLLYPEVF"
     gene            complement(14785..15237)
                     /locus_tag="ACG8KR_001166"
     CDS             complement(14785..15237)
                     /locus_tag="ACG8KR_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HFQ0200549.1"
                     /translation="MAQDDVKSHLDKGLYGTPLVNPEEQHKYLGTFRERCYLSMTVAE
                     MIQPKNKENFLKELKKHPDASILLNGALPLSVQNQYIQLATKNNSRFTVVNDYVTANP
                     EEFGLLLTAKEAVNEPVIDIEKKYPEENSSPAEKEKPKKKKSFWDRLF"
     gene            15387..16337
                     /locus_tag="ACG8KR_001167"
     CDS             15387..16337
                     /locus_tag="ACG8KR_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFQ0200550.1"
                     /translation="MRYGRLVALLVILSSISLFIGVSNVQLSELLQLDGQSLQILLVT
                     RLPRTICLILVGATSSICGLIMQHLTQNKFVSPTTAGTMDSARLGILVAMIFLPGASL
                     IVRSLTAFCFAFAGTLLFLSLTRLFLQKNQLMLPLIGVMLGNIIGSVATFFAYQFQLV
                     QNMTSWLQGNFATVIRGNYELLYAVVPLLLLVWLFAYRFTIIGLGESFAVNLGIDYQK
                     MQFLGIGLVSLASAVMLIMVGSIPFIGVIIPNLVSRIYGDQVHKTIGVTALAGSLFLV
                     FCDLVARVLIFPYEIPVSVIVGIIGGAIFLYLLIRGRRYA"
     gene            16330..17289
                     /locus_tag="ACG8KR_001168"
     CDS             16330..17289
                     /locus_tag="ACG8KR_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFQ0200551.1"
                     /translation="MLKKKPSIFIGMLVLVLFVTGMYLTIHTYGNWHFACALRSKKIF
                     AFILVSLAVSSATISFQTITHNQFLTPSVLGLESLYVLVQTVLFFFIGGVTMLQQTGT
                     VFFLLNVGLMVGLSLLLFFFLLKKMRANLFLLLMSGMILGTLFGSVSTFLQVIMDPNE
                     YDLLQGRLFASFGNIQSQYLLPASLIIIGCWYLLFRYRHVLDVFHLGNEQAVNLGIDT
                     DRFEFLILCCVSLLTGTSTALVGPVTFLGFIVANISYRVFSTYRHGQLFIGSFLVAML
                     FLTGGQLAVEQIFGWNTTVSVVVEFVGGIYFVTKLIRERKSIL"
     gene            17286..18041
                     /locus_tag="ACG8KR_001169"
     CDS             17286..18041
                     /locus_tag="ACG8KR_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0200552.1"
                     /translation="MIDLQKVNKLYHGKSVVKDVQLTIPEKKLTAFIGPNGAGKSTVL
                     SMISRLIPKDTGEIYLDHNEVKAWKSNELAKKLSILRQSNEINVQITVKELVEFGRFP
                     YTKGKLTTKDEEIVQESLEHLGLEEIADQSIDTLSGGQLQRAYIAMVLAQDTDYILLD
                     EPLNNLDMNFAVQIMQILKNLVQEIGKTIVIVLHDINFAASYADEIVAMKDGQLFTQG
                     TTQEMIKKDVLDELYEMDIRICEIEGRRFCLYF"
     gene            18064..19017
                     /locus_tag="ACG8KR_001170"
     CDS             18064..19017
                     /locus_tag="ACG8KR_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HFQ0200553.1"
                     /translation="MKKLVVGILILASFGLAACGNSNNTSQADTKKSSTQTETTLTIT
                     DSNGDQIEVPNNPEKVVVFDNGSLDTMDALGVGDKVVGAATSSLPEYLSSYKKVESAG
                     GIKEPDLEKINQLQPDLIIISGRQRDFQKDLSQIAPTIFLSLDAKNPWESFQQNVTAL
                     GEIFGKQEEAKTQLEELSSAIDQTKKKAEATDKKALVTLVNEGQLSAYGSGSRFGFIH
                     DLFGFEQADDQIEASTHGQSVSYEYVLEKNPDILFVVDRTKAIGGDDSKDDISANELV
                     AQTNAGKNQQIISLEPDVWYLSGGGLESMKLMIEDVNQAFK"
     gene            complement(19540..19836)
                     /locus_tag="ACG8KR_001171"
     CDS             complement(19540..19836)
                     /locus_tag="ACG8KR_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit alpha"
                     /protein_id="HFQ0200554.1"
                     /translation="MKEYIKNIYFIEETQNIEGSYIEVKTLFVNEDKTKALDIYKKLA
                     SKKTNSFGLILSEYKIKAEESYFYQLLKRWSKLPADFYRKMQIINYQPLAETHA"
     gene            20191..20547
                     /locus_tag="ACG8KR_001172"
     CDS             20191..20547
                     /locus_tag="ACG8KR_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HFQ0200555.1"
                     /translation="MKLEVTKEAQEVLKNKLEADDQLLLDIENGDGPFAESQVSCQLD
                     TAFRLIIVKKDTQTDLSLYSVVADTPVGPIKIKDSAILYMDDPSTLALEPTYNSLQLK
                     GPSGLRKGNLQVVRKD"
     gene            20557..21456
                     /locus_tag="ACG8KR_001173"
     CDS             20557..21456
                     /locus_tag="ACG8KR_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFQ0200556.1"
                     /translation="MGKLAIISDLHVDINKFGESELMQLWQVLQKHKITRLHLAGDTA
                     NKVDRCLAVVEFFVNKGLSTTFHFGNHELADVPNEQMMDHFPDPHFLNERYIALNEQT
                     VLLGMNGWYDYQFSELKDYEAIVRMKRLYWYDRFIQRGKTDLEVNAEMLAAAKNLLDQ
                     LEAKKLEVILSTHFVPKRAFIMYQSAPYERWNKLNAFLGSESFGELLDRYSNIKQVVF
                     GHTHRRFEDKSIGHTIYSCRPFGYYYEWQLTRDFVLKEQLLDSYDPMKLRVLLRAHHP
                     AFQAYQTQHLQEEFEQAMTIVPY"
BASE COUNT         7180 a         3827 c         4631 g         6076 t
ORIGIN      
        1 attattaata attcattaca caaagataag aataaccttt gttataaaac gctttattct
       61 gtgtaatgtc cttttttatt aagaaaaact tccatttagg aagcagagag gggtattcga
      121 ttgtccgata accaattatt agaagttcag aacctacata caggattccg actcaaagat
      181 gactattacg atgcagtaga tgacgtttca tttactttag acaaaaacga gattttagca
      241 atcgttgggg aatctgggtg cgggaaaagt accttggcta ctacgattat gggattactt
      301 gatcctaata acacaaaaat tacaggtgag attttatata acgatctgaa tttaatcgac
      361 ctgaacgaaa cgctctataa taaaatccgc ggaaatgata tcggaatgat tttccaagat
      421 ccgttgtctg cattaaatcc tctcatgaga atcgaagacc agatcaaaga aggactttcc
      481 tatcacacaa agatgaatgc agaacagcgt caagctcgtg cactagaatt gctagaacaa
      541 gtagggattc ctaatcctgc gagagttgga cgccaatatc cccatgaact atcaggcggt
      601 atgagacaac gtgtcatcat cgcaatcgca atcgcctgca aacctccaat cgtcattgca
      661 gatgaaccga cgacagccct agatgtaacc atccaagcac agattcttga tttactgaaa
      721 gaccttcagg aagaaacaaa aacagggatc atcttgatca ctcatgactt aggagtcgta
      781 gcagaaatgg ctgatcgagt ggcagtgatg tatggcggac agttcgttga agtagcaggt
      841 gtaaaagaac tgtttgatca tcctaagcat ccttatacac gctctttact gaactctata
      901 ccacaagaag gaaatcatga tgcagaactc catgtgatcg aaggtgtcgt tccttctctg
      961 aaaaacatgc ctcgtaaagg ttgtcggttt gcaccacgta ttccatggat accagcaagc
     1021 gatcatgaag aagagcctat ccttcatgaa gtcgcaccga accacttggt acgttgctcc
     1081 tgctataagc atttccactt tagagacgaa aaaggagagg tgtagatgac agaattaatc
     1141 acaatcaaag acctgaaagt tcattaccct attcgcagtg gattcttcaa tcgagtaacg
     1201 gatcatgtat atgctgtaga tggtgtggat tttattatcg aaaaaggaaa gacatatggt
     1261 ttagtgggag agtctggctc tggaaaatct actacgggaa aagccgttgt gggattagaa
     1321 aaggtaacag ccggacaaat catgtatgaa ggaaaagatg taacgaagcg aagcaaccgc
     1381 aaaaaaatgg gttataacaa ggatgtgcag atgatcttcc aagattcaat gtccagtttg
     1441 aatcctaaaa agcgagtgtt ggatatcatt gctgaaccca tccgaaattt tgaacggtta
     1501 agcgatcaag aagaaaagaa aaaggtaaaa ggactgctag atatcgttgg gatgccggaa
     1561 gatgcgctat ataaatatcc tcatgaattt tcaggcggac aacgtcaacg tctaggagta
     1621 gcccgtgctg ttgctaccaa tccaaaactg atcgtagccg atgaacctgt atctgctctg
     1681 gatctatctg ttcaagcgca agtattgaat ttcatgaaac gaatccagca agaatacggc
     1741 ctcagctatt tattcatttc acatgattta ggtgtagtaa aacacatgtg cgacaatatc
     1801 gcaattatgt acaaaggtcg atttgtagaa atcggtacaa gagaagacat ttataatgac
     1861 ccgcgacata tctatacgaa acgtctgctt tcagctattc cgcaaatcga tgtagaaaat
     1921 agggaaagcc acaaagaaaa tcgacgaaga gtagaacaag agtatatcca aaaccaaaaa
     1981 gaatactatg atgcgactgg tcgagtctac gatttgcgta cgatcacaac tacacacaaa
     2041 gtggcgttga aagatggagg tgcaagctaa tgtggaaaac gattctgcga cgtgttttat
     2101 tgatgatccc gcaagttatt attttaagtg ttctgatctt cttattagcc caagcgatgc
     2161 cgggggatcc atttaccggt ttgatcaatc caaatcagga tccggcagtg atcgaacaga
     2221 tgcgacaagc agctggcttg aatgacccat ggtatgtcca gtacttccgt tggatcggaa
     2281 atgctttgca aggcgatttt ggtcaaagtt tcttatacaa acgctctgtt gctgacttga
     2341 tcggcgaacg gatcatcaac actttatatc tatcgatctt gacagttgtc cttacttatt
     2401 tgattgctgt gcctctgggg atgcttgctg gccggtatca aaattcaatc ttggataaga
     2461 ttgtcgtaat ctacaacttc tttagttttg ctgttccttt atttatcttc ggcttgatca
     2521 tgctatttgt ttttggttat cgtttaggct ggttcccaac aagtggatca caaacactag
     2581 gtttgcaagg tggatttatc actcaatgga tagaccgttt gaaatatatt ctgttaccat
     2641 caattaccca agcatttttg gcgactgccg tcacaatcca atatctgcga agtgaagtaa
     2701 tcgattcgaa atcgctcgat tttgtcagaa cagcccgttc caaaggggta ccgacgaata
     2761 aagtattcag tcgacatatt ttccgaaatg cagcgttgcc gatggcttct caaatggggt
     2821 atgaaatcac ttcgttgatt gctggttctg tagttatcga aaagattttt ggttatccgg
     2881 gagtcggaaa actatttatt gattccatcg gccaacggga ctataccgtg atcacatcct
     2941 tagtattgat tttaggaatc gcgacgctag tggggacatt actttcagat atcatcatga
     3001 gtatcgttga cccgcgtatt cggattcaat aaaaccaatc agaaaggaga atggatcgat
     3061 gagtgaaaaa aacaatacag cagttcaaga aagtattcct cctatgggga tacgaatgat
     3121 tgctcgagaa tttaaaaaag ataaaattgc tattttttca ttaggtttgc tagtggtttt
     3181 attattagtg atctttatcg gtgcgatgct gaccgatcaa gacaaagtga tgtatgttag
     3241 tatcttcgac aaatacgctg caccaggaac agtttcgaca caaggacaaa agttcattct
     3301 gggagcagat gaaggcggac gtgatgtatt aggacaattg atcataggtg ctagaaactc
     3361 tgttacgatc ggatttgcga tcacgatcct tacttcgatc atcggggttg gattagggat
     3421 catctctggc tactacggag ggatcattga caacatcttg atgcggatcg ttgattttat
     3481 catgattcta ccgatcatgc tgatcattat cgtcttcgta tcgattattt ccagatacaa
     3541 tgtctggtcc tttatattca tcatgagtgc attttattgg gtagctaaag cccgattatt
     3601 ccgaagcaag acattatctg aagcaagaag agattatgtc agtgcctcaa aaacaatggg
     3661 aaccaatgat tttaaaatca tgttccgcga aatcatgccg aatctaagtt cattaatcat
     3721 tactaatttg acgatgaact ttgcggcaaa catcggtatc gagacaactt tgactttctt
     3781 aggtttcggc ttgccggcaa atgtacctag tttaggtaca ttgatcggtt atgcaagtaa
     3841 cggagaagtc ttagtgaatc ggcaatggat ctggttaccc gcatcaatcc ttattttagt
     3901 tttgatgttg agtatcaatt atgttggaca agcattcaag cgttctgcag atgcgcgcca
     3961 acggttaggc tagtatagaa gcgaaggggg aaataggaat gaagaaaacg atttttggat
     4021 ttgcagcgct gatgtctgct gtcgttcttg cagcatgcgg gggcggaggt acagatacct
     4081 ctggaaacaa ctctggaggc aacggcaata acaaagagga attaactttt ccaattgcaa
     4141 caaaaaataa tgatgatgca atcgaagacg gcgaattaga agctgcggtc gcaacggaca
     4201 gtcagtttaa aggattgttc cagtgggagt tttaccaaga ttcatatgat gcagctttta
     4261 tggctccttc tcatgaaccg ctatttgcaa acgatgacga tttcagaatc acagacgacg
     4321 gagctgcaac gcttgatcta gatgaagata cgaaaaaagc gacaatcaaa gtaaaagaca
     4381 acgtcaaatg gtcagatggt gaagatgtaa cggcggacga tgtgattttc ccttacgaaa
     4441 tcattgggaa taaagattac actggtatcc gttatgacga cactttccgt aacatcgtag
     4501 gtatggaaga atatcatgat ggaagtgccg acacaatctc cggaatcaaa aaaatagacg
     4561 acaaaacagt tgaaatcgaa tataaagaag taaacccaag catgcttcaa tcgggtggtg
     4621 gtatctggtc atatgcagca ccaaaacata cgttagaagg tgtagcaatc aaagatatgg
     4681 aatcaagcga tcaagtacgt aaaaatccag tgacttttgg cccttattac atgagtaata
     4741 tcgttgcagg agaatcagta gaatacttgc caaacgagca ctactggaat ggtacgccta
     4801 aattgaaaaa gatcacgatg aaatctgtac cgatggcaag tgcaactgaa gcattgaact
     4861 ctaaattata tgatatgatt ttccaaatgc caacagatac ttacgatacc tataaagaaa
     4921 ttgctggcta tacaaactta ggccgtgaac aaacttctta tacttatcta ggattcaagt
     4981 taggtaaatg gaatgcagat acaaagagtg tggaatacaa tccagattca aaaatggctg
     5041 ataaatcatt acgtcaagca atgggctatg cattagataa taatgcagta ggtgaacgtt
     5101 tctatgcagg tttgagaagc aatgcgactt cattgattcc tccagtattc cagtcatacc
     5161 atgatgatga gatgaaagga ttcacacaag atctagacaa agcgaaaaaa ctattagatg
     5221 atgctggcta caaagatgtg gatgacgatg gtttaagaga agacaaagat ggaaataaac
     5281 taacaatcaa ctttgcttcg atgtccggtg gggaaacagc gcaaccattg gcagattact
     5341 atatccaaca atggaaagaa attggtttgg atgtccaata cacaacaggt cgtctgatcg
     5401 aattcaattc tttctatgat aaattagaaa acgatgatcc tgaaattgat gtttaccaag
     5461 ctgcttggaa tacaggaact gatccaaatc caagtggact atggggacca aatgctgcgt
     5521 tcaactacac tcgttatgaa tcagaagaaa atacaaaatt gatcaacgat atcagttcaa
     5581 gcaaagcatt tgatgaagag taccaaaaag aagcatatca aaaatggcaa caatatgcat
     5641 ttgaagaagc atttgcgatt ccaacgttgt tccgtaatga agtattgcca gtcaacgacc
     5701 gtgtcaaaga ctgggattgg tcttatgatg taccaaaccc atgggcaaat gtttctgtta
     5761 cagcagattc acgttcataa tcactaaaaa tgaccgaaaa agggcgatgt cctttttcgg
     5821 ttttttgata tttttctgtt tatttctcta atattcaggt caaggcgctg atttcatgac
     5881 ttctccaata aacggtgttc aaagtccagt ttctcggtat taagtcaaat ttatgcaaaa
     5941 atatggaaag ccattttcac aaaatttttt cttcatactc gaaactaaac gaatttttca
     6001 caacttctcg tcaatcacta ggattattaa agctttactt accttttgac cgaaccattt
     6061 caaatttgac aaaaaaccag tataataagg aagtatcgaa aaaatatcca taaaaggaat
     6121 gtggaaaatg gacaaccagc tgacaaaaga actaaaagaa aactataaca tcgtttttca
     6181 cgatctaaat ttgcttgaac aagcttttac ccattcatcc tatgtgaatg agcatcgcaa
     6241 tctgcaatta tcggataatg agcgacttga atttttagga gatgccgttt tagaactgat
     6301 ggtttctcag tatttattcc gtttatatcc tcatttgcca gaaggaaaat tgacaaaaac
     6361 acgagctgcg atcgttcgcg aagacagctt gtcgaaattt gccaaagaat gtcactttga
     6421 tcaatatgtt ttgttgggca agggagaaga gaattcagga ggacgtacgc gaccagcatt
     6481 gctttgcgac ctattcgaag cttttttagg cgcgttatat cttgaccagg gattcgacac
     6541 gacccatgca ttcatcgaaa aagtgatttt tcctaaagtc aaagcaggtg ctttttcaca
     6601 tgagatggat cataaaacaa aactacaaga agtcttgcaa aaatcaggtg atgtatcaat
     6661 cgaataccga ttgatcaatg aagaaggtcc agctcatgaa cgagtatttt ggatcgaagt
     6721 ttatgtagat gatcaattga ttggcactgg acaagggaaa tcgaaaaaat tagcagaaca
     6781 agctgcggcg gaaaatgcat tggcagcact gtaaaagtaa gtgagggaaa gctttgtatt
     6841 tgaaacgaat tgaaatagcc ggttttaaat catttgcaga caagacagtc attgattttg
     6901 aaaatagcgt cacagcagtg gttggaccaa atggcagcgg aaaaagcaat attacagagg
     6961 ctatccgctg ggtattagga gaacaatctg ctaaaagtct gcgaggcgga aaaatgccgg
     7021 atatcatctt tgccggttcc gatacccgta agcagctgaa tattgcagaa gtcacagtga
     7081 ttttagacaa tagtgatcat tatctgccat tggagtacaa tgagatcagt gtaacccgcc
     7141 gttatcgacg gactggggaa agcgagtttt tcatcaataa acaaagctgc agattaaaag
     7201 atatccaaga attgttctta gattctggat taggaaaaga atcgttctca atcatttctc
     7261 aaggaaaagt agaagcgatt ttcagtagta aaccggaaga ccgtcgtggg atctttgaag
     7321 aagcagctgg tgtcttgaaa tacaagcagc ggaaaaagaa agcagaacag aagctttttg
     7381 aaacagaaga caacctcagc cgcgtccaag acatcatcca cgaattggaa gaacagttaa
     7441 ctcctttggc cgcccagagt gaggcagcaa aggagttttt acggctaaaa gaaacactga
     7501 ctcaaaccga tgtctctttg atggttgctg aaatcaagac agcaaaaaaa gattgggaca
     7561 acaaacaagc acagcttgcg aaattcaatc tagaattagg caaactatct gagtcgatcc
     7621 aagaacaaga atccattttg gcgaagcagc gtaaagaaaa tgcgcaagcc gatcgtctga
     7681 tcgaaaaaaa tcagcaagta ttattagatc tttcggaaaa actgaagcag acagaaggac
     7741 aaaaagatgt tttgcaggag cggacaaagc atacacaaaa aagctcgcaa gaatatcaaa
     7801 cttcattagc agaagcacaa aaaaaggtta aacattttga aaagcttcaa gaaagcttga
     7861 tgaaagcagc agcagaaaaa gaaacagaga tccaaaaagc tgaagcaaat ctcataaaaa
     7921 cacagcaaga attagaaaaa taccaaaaat caacgaaaga attattggca gaattaagag
     7981 atcaatatgt tgatttgatg caagaacaag cagctgtagg aaacgaactg aagtatctag
     8041 aacgtcaata tatacaagaa acagcaaaaa gcaagcagac gttagccaag caatcggaag
     8101 tagaagcatc agtagaccga ttgatcttac aaaaagaaga attgacacaa aagcaggcgc
     8161 agctaaaatc atctttaact gaaacaaaag agaagttaga aatgatccaa caaaacggga
     8221 aaaagttcca agaagcatta gcaaaagaac agcctaaaat gtatcaatta atgaatcaag
     8281 tccagcagct acgtgctcgt aagaaaagcc ttcaagaaat ccaagaaaac tattttggtt
     8341 tttaccaagg ggttcgattg gtcttgcagc ataaacagca gctttcgggt attgtcggag
     8401 cagtggctga actgatcgat gttcctgcag atttcacgtt agctatcgaa gcagctttag
     8461 gtggggcagc ccagcatgtc atcgttgaaa atgaaaaaga tgctcggcaa gccatcacgt
     8521 atttgaaaca gcaaagagga ggaagagcaa cattccttcc actgacgaca atcaaaccaa
     8581 gacaattacc ggctcatata ttgacacaag cagcagctgt agaagggttt atagggattg
     8641 ccagtgaaca agtcagttat cccgatcaaa tccaaactgt ggttcaaaac ctgctaggaa
     8701 cgatcctttt agccaaagac ttaactagtg ccaatgcaat cgcgcaaacg atccgctatc
     8761 aatatcgagt ggtttcctta gaaggtgatg tgatgaatgc cggaggatcc atgacaggtg
     8821 gagcgaataa acgaggaaac caaggcagtt tgtttgtaca aaatcaagaa ttgaaacagt
     8881 tgactagtga atttgaagaa gcagataaac aactccaagc acaagaaaaa aaagttcagg
     8941 agctgcaaca agaaacagct cgtttagccg aagaacaaga agttttgcgt acacgaggcg
     9001 aacaattacg ttttgaagaa caagaagcga caaatcagct acaaaacatc ataaatgaac
     9061 ttgaacgttt tgaaaaagaa aagcaaatct caacttttga gacacgtgaa ctgcagcagt
     9121 ttatcgaaga ttacgaaaag caaacgaacg aattgaaaga caaacaaaca gatcttgaat
     9181 cgcaacgaca aaaaatcgac gaagaaatca aatcgttaag tcaagaaagc gaccagatgg
     9241 aagccagaag agcacaagta caaagtcaaa aagcacaaga acaagcagat cttgctgtct
     9301 tgaaagagca gtttaatcat ctgcagatcc agctccgcgg tgcccgagtc caaaaagcag
     9361 aggcaaccga acgtcaagaa gcgatagaaa agcagttggc gacattgaca gcagactttt
     9421 ctgatcatga agtcacagaa gaaagtttgg aaaaacaaat taacgaatta tctgctcagc
     9481 gagaaacatt aaaagccgag ttggcaaaag ccaaagagca gcgagataga aggcaaaagg
     9541 agatcgataa gcttgaagca gtcttggcag agcgtaatcg tgagcaaaaa gcacgactgt
     9601 cggaacaatc caaattagaa gtccaaaaag accgagctga aatgatttta gataatcatt
     9661 tgacctatct gcagtcagaa tatcagctga catttgagaa agcttcccaa gactatcaag
     9721 aaacaaccga tatcgaagac agtagaacaa aggtttcatc cttaaaagaa cagattgaaa
     9781 aactgggacc agtaaactta aattcaatcg aacaatacga acaagtcagt gaaagacata
     9841 cattcttagc aacacagcga gatgatctgt tagcagcgaa aaaccaattg tttgaaacga
     9901 tggatgagat ggatgatgaa gtgcggacaa gattcaaaga agtatttgaa gctatccgtc
     9961 aagaattcaa aattgtcttt ccaaatatgt ttggcggcgg taaagctgaa ttagtactga
    10021 cggaaccaac tgatttgctg aaaacaggta ttgagattga agtacaacct cctggcaaaa
    10081 aactgcaaag tctaagtttg ctttctggag gagaacgagc acttacagcc atcgcgttgc
    10141 tcttttccat tatccgtgtc tgtcctgttc cgttttgtat cttagatgag gtagaagcag
    10201 cgttagatga agccaacgtg aaacgttttg gtcgttactt gagcgatttc caagatgaca
    10261 cacaatttat cgtagtcaca caccgcaaag gaacgatggt cgcagcagat gtactgtacg
    10321 gagtgacgat gcaggaatcc ggtgtatcaa aaatcgtttc tgtacggatg gaagatatca
    10381 atgaagaggc gaaaatcaac gaaacgaaag tgtaggaacg aaaatgatca aattagtagc
    10441 aatcgatttg gacggtacat tattaaatga acaaaagctg atttcagatg agaataaaca
    10501 agcattagca caagcaaaac agcaaggggt caagatcgtt ctatgtacag gacgcccctt
    10561 agctgcgatg gcgcattatc tgcaagaatt aggactggta gatgaaggtg actacagcat
    10621 cactttcaac ggcggtttgg tacaaaaaaa tgatactggt gaaatcatgg aaaagaaagt
    10681 gatggaagta tcagatatcc aacgcttata caaactggct cagcaattgg atctgccact
    10741 agatgttttg tctgacaatg tggttttaca gctgcctagt gccccaagga agcaatcctt
    10801 gtacaatgtg ttgaataagc tgctgcaatt ccaaccagcg gatataagcg atattacaga
    10861 tgaatttgtg cttaataagg cagtaatcgc ttatgaccaa gaagaattag atcctaaaat
    10921 caaagaaata ccagcagaat tccatgaaat gtatgagatc atcaaaacaa gaagtatgct
    10981 tttggaattt atgccaaaag gtgttacaaa agcttatggt atttctttat tggcaaaaga
    11041 tcttggtttg aaacaagaag aaatcatggc gatcggtgat gaggaaaatg atttaccgat
    11101 gatccaatat gctggactag gcgtagcgat ggagaatgct gtgcctttcg tcaaagaagc
    11161 agcagatttc gtgacagcca gcaatgaaga aaatggcgta gccaaagtga tccaaaaatt
    11221 tgtattagag tagggggagt caaatgggat tttttgataa aatcaagcgt gcttttactg
    11281 gcgaagagaa aacagaagaa gttgaagtac aggagaagta cgacaagggt ttagaaaaga
    11341 cgcgtaaaac atttggtgaa cggatgaatg agttgtttgc caattttcga acagtcgatg
    11401 aagacttttt tgaagaatta gaagaaacac ttatcggtgc agatgtaggt tttgaaacag
    11461 cgatacgaat caccgaagca ttacgtcaag aagtgaaact cagaaatgcg aaaaaatcgg
    11521 cagaagtcca aaatgcgatc atcgagaaac ttgtggatct ctatgaagcg gaaggaatca
    11581 atgaagtaaa tgaactaaat atccaagaag acggactaac agttattttg tttgtcggtg
    11641 ttaatggtgt agggaaaaca acaagtattg gtaaacttgc ccatcagttc aaaaacgaag
    11701 gtaaaaaagt attgatggca gcagcagata ccttccgcgc tggagcaatc gaccagttag
    11761 ttgtctgggg cgaacgcgct ggtgttgaag ttgttcgtgg caatgcagga ggcgatccag
    11821 cagctgtcgt attcgatgct ttggaaagag caaagacaga gcaggcagat gttttactag
    11881 ttgatactgc aggccgactg caaaataaag tgaacttgat gaacgaatta gaaaaaataa
    11941 aacgagtaat ccagcgggaa ttaccagaag caccccaaga aacacttctt gtattagatg
    12001 caaccactgg tcaaaacgcg atggttcaag caaaacaatt caaagaaaca acagatgtga
    12061 ccggtcttgt tttgacaaaa ttagatggta cagcaaaggg cggtatcgta cttgctatcc
    12121 gtaatgaatt gcatttacca gtgaaattgg tcggtcttgg tgaaggaatc gatgacttgg
    12181 agccgtttga tccaaatgat tttgtcgttg gattgttcaa aggattacta aaagaagaat
    12241 aacccatgtc taaaaagcag gagctgtgac aaaagttgca gctccttgtt tgaatgattg
    12301 tttttaacat agctgtgttt ttgatgcctt catttttata cttatttgta agcggttctc
    12361 aaacaatttc gttctttttc ttagactttt tgattataat tagattcata tgaattggag
    12421 gtcacttaat gatgaatttt aagcaattat tattgcatgt caatgcgcgc ccttttatcg
    12481 accaagctcg atttggtata gaacgggaag gacagcgggt tgatcttgca ggaaatctag
    12541 caaaaaccga tcatccagca atctttggcg atcgatccta tcacccctat atccaaacag
    12601 attttagcga aacacaaaca gagatgatca cccctgttac cgattctatt cccgaattat
    12661 ttcagtatct cgctgctgtt tatgatgtga ctgctcgttc tataccgaaa gaagagatga
    12721 tctggccatt aagtatgcca cctgccttac cggaaaaaga cgaagagatc attattgcaa
    12781 aattaaaaaa tttcgaagat gtcttgtatc gacgttattt agcaaaaaaa tacgggaagc
    12841 gaaaacagat ggtaagcggt attcatttca attttgaatt tggcgatgag ttgctaagaa
    12901 cattgttcag ccatcaggaa gaatttcaag atttttccga attcaaaaca gaactttatt
    12961 tgaaaacagc gagaaacttt atgcgctatc gctggatgat cacttattta ttcggtgctt
    13021 caccaatgag tgagaagaac tactttttag atgaatcaca tccgcaagaa cctgttcgca
    13081 gtattcgcaa cagtgcgctg ggctatacga atcatccaaa tgtgaaagtt tcctatgctt
    13141 ctatgaaaca gtatttagca gatatcgagc gaatgatcga agaagggaaa ctttcggagg
    13201 agaaagaatt ttatacaccg cttcgtttcc gaggcggaaa gaaagtcgca gatttagcaa
    13261 caacaggtgt tcgttatatt gaattgcgga atatcgattt gaatccttat gcaagattgg
    13321 gtatcaatcc agagcaagtt cggttcttac aattattcct gatgtacatg ttatggacag
    13381 aagaaaaaga agactgtgac caatgggtag cagaaggtac gactcgaaat aacaaagtag
    13441 cactagaaca accatcagat caaacagaat ttcatcaaga aggtagagaa attcttgaag
    13501 gaatgaagca aatgctggtt gaattggatt ggttggattc tctttattta gtagaggaag
    13561 cattgactca aatggatcat cctgaacaaa cgttagcagc gaaactctat caagaagcac
    13621 agctatcgag tcagcaagaa gttgctgtgg cattgggaca tcaatattat aaagaaagcc
    13681 atgaacgtcc ctatcagttg gccggttttc gcgaaatgga actttctact cagatattta
    13741 tgttcgatgc catccaaaaa ggtgtccaag tcaaagtatt agatgagtcg gatcaatttt
    13801 tgcgtctgca attccaagac catgtagaat atgtgaaaaa tgcaaacatg acaagtaagg
    13861 acagctatat cgtcccactt atcatggaaa acaaaacagt aacgaaaaaa gtcttaaaag
    13921 aagctgggtt ccgggtacca ggaggcgcag aattttcatc catggaagaa gcggtaaaag
    13981 cttatccgag atttgccgag caggcatttg tgatcaaacc taaatcaacc aattacgggt
    14041 taggtatcac gattttcaag gaaggagcta gtctggaaga ttatcaagca ggtttagcca
    14101 ttgcttttcg tgaagacagt tcggttttag tagaagaatt tatgccggga acggaatatc
    14161 gtttctttgt gatcgacgga gaagtccaag ccatcatgtt gcgagtccct gctaatgtta
    14221 ttggtgacag tatccgaaca gtgaaagaac ttgttgaaga aaaaaatagt gaccctttgc
    14281 gtggaacgaa tcatcgtgca cctttagaat tgatccaatt aggtgaatta gaacaattga
    14341 tgttgaaaga acaagggttg acgatcgaat cagttcccca agccaatcaa attgtttatc
    14401 taagggagaa ctcaaatatc agtacaggtg gtgattcgat cgatatgact gatgaatttt
    14461 ctgaagctta caaaaaaatc gccgtttctg cggtagaagc actaggagct aagattagtg
    14521 gtatcgattt gattatacca gataaagaaa tcgatcctac aactgataaa aaagcatatg
    14581 ggatcatcga agcaaatttc aatccagcca tgcatatgca tgtttatcct tttgcaggaa
    14641 aaggcagaag attgacgatg aatgtattga aattgttata tcctgaagta ttttaaaaga
    14701 aaaagctttt gacagattgt cctatttgat gtcaatctga tcaaaagctt tttttgcatg
    14761 taaaaattta tgtgtttttt cgttttaaaa taaacgatcc cagaagcttt ttttcttttt
    14821 tggtttttct ttttcagctg gcgaagagtt ttcttctggg tatttttttt cgatatcaat
    14881 cacaggttca tttactgctt cttttgctgt taagagcaat ccgaattcct caggatttgc
    14941 agtgacgtaa tcgttgacta cagtgaaacg gctattgttt ttcgttgcta gctggatata
    15001 ttggttttgg acagaaaggg gtagagcccc attcaagagt atggaagcat ctggatgttt
    15061 tttgagttct tttaaaaagt tttctttgtt ttttggttgg atcatttcag caacagtcat
    15121 actaagatag cagcgttccc tgaatgtccc taaatattta tgctgttctt cagggttcac
    15181 taaaggtgta ccataaagtc ccttatccaa atggcttttt acatcatctt gtgccataaa
    15241 aaataccctc cttcttctct ttgtctttct tacgattata gcatattccc aataatacct
    15301 aaaacatatg aaaaagcggt tgcttttgtt caacgattca cttaaaataa gaatgaaaat
    15361 cattctcaat tagaaaggtg gattggatgc gctacggacg actagtagca ttattagtta
    15421 ttttaagtag tatttcattg ttcatcggag tatcgaatgt acaattgtca gaactgctcc
    15481 agttagatgg gcaaagctta caaatcttgc tagtgactcg tttgccacga acgatttgtt
    15541 tgatccttgt gggagcgacc agtagtattt gtgggttgat catgcaacat ttgacccaaa
    15601 ataaatttgt ttcaccaacg actgcgggca cgatggatag tgcgcgttta ggtattttag
    15661 tggctatgat tttcctacct ggtgcatcct tgattgtccg atccttgaca gcattttgct
    15721 ttgcttttgc aggaacattg ctttttttat cccttacgcg attgttcctc cagaaaaatc
    15781 aattgatgct tccgttgatt ggagtcatgt tagggaatat cattggttca gttgctactt
    15841 tttttgctta tcaattccag ttggtacaaa atatgacttc atggcttcaa gggaattttg
    15901 cgactgtgat acgaggaaat tatgagttat tgtatgctgt tgttccattg ttgctcttgg
    15961 tttggctatt tgcttaccga tttacgatca ttggtctggg cgaatcgttt gcagtaaatc
    16021 tgggaataga ttaccagaag atgcagtttt taggcattgg cttagtatca ttggctagtg
    16081 cagtcatgtt gatcatggta ggaagcattc cttttatcgg agtgatcatt ccgaatcttg
    16141 tctctagaat atatggagat caagtgcaca aaacaattgg cgtaactgct ttagcaggta
    16201 gtttgttctt agtattttgc gatcttgtcg ctcgcgtgtt gatttttcct tatgaaatac
    16261 cagtcagtgt gatcgttgga atcatcggtg gggctatctt cctttatctt ctgataaggg
    16321 gacgacgata tgcttaaaaa aaagccatct atttttattg gtatgcttgt tcttgtttta
    16381 tttgttacag gaatgtactt gacgatccat acatatggaa actggcattt tgcatgtgca
    16441 ttgaggagta agaaaatttt tgcatttatc cttgtctcgc tagctgtcag cagtgccaca
    16501 atcagctttc agacaattac gcataaccag tttttgactc cttcagtatt gggacttgag
    16561 tcgttatatg tccttgtcca aacggtcctt ttcttcttta tcggaggagt gacgatgctt
    16621 cagcagacag gtacagtttt tttcttattg aacgttgggc tgatggtcgg gctgagtcta
    16681 ttgttatttt tctttctttt gaaaaaaatg agagcgaatc tttttttatt actgatgtca
    16741 gggatgattt taggtacgct ttttggtagt gtcagtacct ttttacaagt catcatggat
    16801 ccgaatgaat atgatttgct tcaaggcaga ttatttgcaa gctttggcaa tatccagagt
    16861 cagtatcttt tgcctgcttc tttgatcatt atcggctgct ggtacctgtt atttcgatat
    16921 cgacatgtgc tagacgtttt ccatctcgga aatgaacaag cagtaaacct cgggatagac
    16981 acagatcgtt ttgaattttt gattttatgt tgtgtcagtc ttttgacagg gacatcaacg
    17041 gctcttgtgg gtccagttac ttttttaggg tttattgtcg caaatatcag ttatcgtgtc
    17101 ttttctactt atcgtcatgg tcagctattt atcggaagct ttctggtagc tatgctgttt
    17161 ctgacaggag gacagctcgc agtagagcag attttcggct ggaatacaac agtgagtgtc
    17221 gtcgttgaat ttgtcggagg aatttatttt gtcacaaaat tgattcgcga aaggaagagt
    17281 atcctatgat tgatttacaa aaggtcaata aactgtacca tggaaaatca gtggttaaag
    17341 atgtacagtt aacgattcct gaaaagaagt tgactgcttt tatcggtcca aatggtgctg
    17401 gaaagtcaac ggtgttgtcg atgatcagtc gtttgatacc aaaagataca ggtgaaatct
    17461 atttagacca taacgaagta aaggcttgga aatccaatga attagccaaa aaattatcga
    17521 tcctacgaca atcgaatgaa ataaatgtcc aaatcaccgt gaaagaatta gtagaatttg
    17581 gacgatttcc ttatacgaaa ggaaaattga cgacaaaaga cgaggaaatc gttcaggaat
    17641 cactggaaca ccttgggtta gaagaaatag cggatcaatc gattgacact ctttcaggcg
    17701 gtcaactcca acgtgcgtat attgcaatgg tgctagcgca agacacggat tatattttac
    17761 tggatgaacc tttgaacaat ctagatatga attttgcggt tcaaatcatg cagattttaa
    17821 aaaatttagt acaagaaatc gggaaaacaa ttgttattgt tcttcatgat atcaactttg
    17881 ctgcaagtta tgcagacgaa attgttgcaa tgaaagatgg acagttattt acgcaaggaa
    17941 cgacccaaga aatgataaaa aaagatgtat tggatgaatt atacgaaatg gacatacgga
    18001 tttgtgagat tgaaggccgc agattctgtc tttatttcta aaactaacat taggagagaa
    18061 gagatgaaaa aattagtagt aggtatcttg attctagcat catttggttt agcagcatgt
    18121 ggaaatagca acaacaccag tcaggcagac acaaaaaaaa gcagcacaca aactgaaact
    18181 acattgacga taacagatag taatggagac caaatcgagg tgccaaataa tccagaaaaa
    18241 gtcgtcgtat tcgacaatgg ttctttagat acgatggatg ctcttggtgt tggagataag
    18301 gtagttggag cagctacaag cagccttcct gagtatcttt catcctataa aaaagtagaa
    18361 tcagcaggtg gaataaaaga accagactta gaaaaaatta atcagcttca gccagatcta
    18421 atcatcattt ctggccgaca aagagatttt caaaaagatc taagtcaaat tgctccgacc
    18481 attttccttt ctttagatgc gaaaaatcca tgggaatctt tccagcaaaa tgtcactgct
    18541 ttaggggaga tctttggtaa gcaagaagaa gctaaaactc aattagagga attatctagt
    18601 gcaatcgatc agacgaaaaa gaaagcagaa gctactgaca aaaaagcact agtcacactt
    18661 gtgaatgaag ggcaattatc agcctacgga tctggttcta gattcggttt catccatgat
    18721 ttatttggct ttgaacaagc agatgatcaa atcgaagcat ccactcacgg tcaaagtgtt
    18781 tcttatgaat atgtgctgga aaaaaatccg gatatcttat ttgtagtaga cagaacaaaa
    18841 gctattggtg gagacgacag caaagatgat atttctgcca atgaactggt agcacaaacg
    18901 aatgctggta aaaatcagca gatcatctcc cttgaaccag atgtctggta tttaagcggg
    18961 ggcggtttag aatcaatgaa actgatgatc gaagatgtga atcaagcatt taaatagcat
    19021 aaataacaga ggattaaact ctcatactat gtaacaataa tgtttcttac attcttaaat
    19081 agcaagagcc tatcctcaaa gaagttatca aaaaccatcc gtaaaatcgt attgatgatt
    19141 ttacggatgg tttttattta tttgagaaat cttttttcaa cgtgaattca ttatctacat
    19201 catacgggtt gattctagaa tggggagtgg caaaaaatag tgtgatccat tttttcagca
    19261 tcaagatcgc ctcctttgat ttgaatcaaa ggtacaaagc gaaacttaga taaaacttaa
    19321 atttagatat ttttattttg taccgatctc ttctttaaac gatgttccat ctgctgcttc
    19381 tcggcaataa gtctactaga ctagaaaata tgagaagcta ttttctgtcc agcgttctta
    19441 ttactcaaag caaatcgcag ttgtcacaat ctcttctaaa caatgttcaa tcagctgacc
    19501 ttcggtatta gctcaaagca ctgatttcac aacctctatt taggcatgtg tctcagcaag
    19561 cggttgatag ttgatgatct gcatttttcg ataaaaatcg gcgggaagct tactccaccg
    19621 ttttagtaat tgatagaaat aactctcttc tgctttgatt ttatactcgc ttaatatcaa
    19681 tccaaaacta ttcgtttttt ttgaagctaa ttttttataa atatccaatg ctttggtttt
    19741 atcttcattg acaaagagtg tttttacttc aatataagaa ccctcgatat tttgtgtttc
    19801 ttcgataaaa tagatgtttt ttatatattc cttcatagtg attcctccaa agaagtcttt
    19861 tatttgataa gtttactcta ccgaagagtt gttaataatc cgtgaacaaa aagtaattct
    19921 ttttccaata gttgttaaag gttttttatt ttttatagta ttttttgtta tttaagaaag
    19981 gaataaggtt aaacaaataa caggttgctt tttgggtctt tttccgttca agaaactgaa
    20041 cgaagaaaag ggaaagttgt ctcttttgtg ataagagaaa tcataataaa cgaatgactt
    20101 actttccaat aaattgagaa cagtcagaac cagacaaatt agttgcatct gatgaataaa
    20161 gtgattattc tatgacatga ggtgatacag atgaaattag aagtgacaaa agaagcacaa
    20221 gaagtattga agaataaatt agaagcagac gatcaacttt tattagatat cgaaaacggt
    20281 gacggtccat ttgcagaaag ccaagtcagt tgccaattgg atacggcatt tcgattgatc
    20341 attgtcaaaa aagatacaca aacagatcta agtctttatt ctgtagtggc tgatacacca
    20401 gttggaccaa tcaaaatcaa agacagtgca atactttaca tggatgatcc atccacactt
    20461 gctttagaac caacttataa tagtttacaa ttaaaaggac caagcggctt gagaaaaggc
    20521 aacttgcaag ttgttcgaaa agactagagg gataaaatgg gaaaattggc gatcatcagc
    20581 gacctacatg tggacatcaa taaattcggc gaatcagagc tgatgcagct ttggcaagtt
    20641 ttgcaaaagc acaagatcac tcggcttcat ctagccggag atacggctaa taaagtagac
    20701 cgttgcctag ctgttgtcga atttttcgtc aataaaggat tatctacgac atttcatttc
    20761 ggcaatcacg agttggcaga tgtgcccaat gaacagatga tggaccattt tccagaccca
    20821 cattttttaa atgaacggta tattgcttta aatgagcaga ccgttctttt agggatgaat
    20881 ggctggtacg actatcagtt ttccgaattg aaagattacg aggcaatcgt acgaatgaag
    20941 cgtttgtatt ggtatgatcg gttcatccag cgtgggaaaa cagatctcga ggtcaatgca
    21001 gaaatgcttg cagctgcaaa aaatttgcta gaccagctgg aagcaaaaaa acttgaggtg
    21061 attttatcca ctcatttcgt tcctaaacgg gcatttatca tgtatcaatc tgctccttat
    21121 gagcgttgga acaaattaaa tgcattttta ggatcagaat cattcggaga attacttgac
    21181 cgttattcaa atatcaaaca agtagttttt ggccatacgc atcgtcgctt tgaagataaa
    21241 tccatcggtc acacgattta cagctgcaga ccttttggtt attattatga atggcagctg
    21301 acccgagatt ttgtcttgaa agaacagttg ctagacagct atgatcctat gaaattacgt
    21361 gtcctcttgc gtgcccatca cccagctttt caggcctatc aaacacaaca tttgcaagag
    21421 gaatttgaac aagcgatgac aatcgttccg tattgagaca gcattagtaa tgtgtaaaag
    21481 aaaatacctt gtaaaacata aggataagta agagcagtag atccatttat cgtggatatg
    21541 ctgctttttt tgcggaaaat aatcagcgaa tgtttatttg atcggatgat ttgtaactcg
    21601 cagagtacta gatgcaagcg tgtattcttt ttttgaacga taatcacaaa aaaaaatata
    21661 atatacattt tttatttaat tataattaat attgattgaa gtctaggcag tcaa
//
LOCUS       DAZKLD010000039        21271 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.039, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000039 DAZKLD010000000
VERSION     DAZKLD010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21271)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21271)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21271
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.039"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(27..1523)
                     /gene="lysS"
                     /locus_tag="ACG8KR_001174"
     CDS             complement(27..1523)
                     /gene="lysS"
                     /locus_tag="ACG8KR_001174"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HFQ0200557.1"
                     /translation="MTQEQQHQVEDLNDQMLVRRQKMEQLREDGIDPFGKRFERTHNS
                     EELHELFDPRTKEELAEMGLTASVAGRMMTRRGKGKTGFAHLQDREGQIQIYVRKDQV
                     GDEAYEVFKHADLGDFFGVTGQIMKTDTGEVSIKASEITILSKALRPLPDKYHGLTNI
                     EQRYRQRYLDLISNRESFDRFMKRSQIISEIRRYLDGNGYIEVETPVLHNEAGGAAAR
                     PFITHHNALDIDLYLRIALELHLKRLIVGGMEKVYEIGRVFRNEGIDTTHNPEFTMLE
                     AYTAYTDFNDVMNLTEGIIRNAAEKVLGTAKITYDGQAVDLESDFKRIHMVDAIKEQT
                     GVDFWQEMTLEEALALAEKHNVEVTEAMGVGHVINEFFETFVEETLTQPTFVYGHPVE
                     VSPLAKKNPEDPRFTDRFELFIVGREFANAFTELNDPIDQRERFEAQEKERELGNDEA
                     HGVDEDFLEALEYGMPPTGGLGIGIDRLVMLLTDAQSIRDVLLFPTMR"
     gene            complement(1634..2638)
                     /gene="dusB"
                     /locus_tag="ACG8KR_001175"
     CDS             complement(1634..2638)
                     /gene="dusB"
                     /locus_tag="ACG8KR_001175"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HFQ0200558.1"
                     /translation="MEHTWKIGNVEIPNRVVVAPMAGITNSAFRVTVKEFGAGLVVCE
                     MISDQGIHFRNKKTLEMLHIEETEHPLSLQIFGGNKDSLVEAAQFVEENTAADIIDIN
                     MGCPVNKVIKAEAGAKWLLDPEKVYEMVQAVSSAVNIPVTIKMRIGWDEEHVFAVENA
                     LAAQSAGASAIAMHGRTRVQMYEGKADWEVLKEVKKHLTIPFMGNGDVKTPEDAKWML
                     DYVGADGVMIGRAALGNPWMIHRTQHYLETGELIPEPTPAEKIATAKLHLQRLADLKG
                     EKVACREFRTHAAYYLKGIPRAAKVKVAINQTEKQQEINDLLDQFVEKAEKVLLKQTL
                     "
     gene            complement(2639..3526)
                     /gene="hslO"
                     /locus_tag="ACG8KR_001176"
     CDS             complement(2639..3526)
                     /gene="hslO"
                     /locus_tag="ACG8KR_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HFQ0200559.1"
                     /translation="MKDYLVKALAHDGFVRAYAVQATNTVAEAQRRHDTWHTSSAALG
                     RTLVGSLLLGATLKGEDKLTVRIQGDGPATGIVVDANGKGDVKGYIKNPHLSLPLNSE
                     GKIDVRGAVGTQGMFTVTKDLGLKEPFSGQTPIVSGEIAEDFTYYLAISEQIPSAVGL
                     SVLVNTDDSIKTAGGFMIQIMPGADEETISKIEEQLKTTPRISTLLDEGQTPEEILKG
                     LLGTEELNILEKMPVQFKCDCSKEKFAEAIIALGAEEIQAMIDEDHGAEAVCSFCNNK
                     YKYSEEELDGLKKEALGGI"
     gene            complement(3743..5854)
                     /gene="ftsH"
                     /locus_tag="ACG8KR_001177"
     CDS             complement(3743..5854)
                     /gene="ftsH"
                     /locus_tag="ACG8KR_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HFQ0200560.1"
                     /translation="MNKKNNGMMKNALYYVLVILAMVMVVYFIFGNNGQRSSDIEYSK
                     FTEQLQAGKIKEFEVQPANGVYKITGEYKEQQTVANSGGLAILGSTDTTTTHFTTIIL
                     PNDTTLSQVTELAKENKVATTVKEESSSGIWVSLLLSFLPIVIIIFFFYMMMGQQGGG
                     GGGGGRVMNFGKSKAKEADKKANRVRFSDVAGAEEEKQELVEVVEFLKDPRRFVELGA
                     RIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFETA
                     KKNAPAIIFIDEIDAVGRQRGAGMGGGHDEREQTLNQLLVEMDGFDGNEGVIVIAATN
                     RSDVLDPALLRPGRFDRQILVGRPDVKGREAILRVHARNKPMADDVDLKVVAQQTPGF
                     AGADLENVLNEAALVAARRNKKKIDASDIDEAEDRVIAGPAKKDRVISKKEREMVAYH
                     EAGHTIVGLVLSRARVVHKVTIIPRGRAGGYMIALPKEDQFLMTKEDMFEQIVGLLGG
                     RTAEEIIFNVQSTGASNDFEQATALARSMVTEYGMSDRLGPVQYEGNHQVFVGRDYGQ
                     TKAYSEQVAFEIDQEVRKILMEAHDKAREIIEAHRAQHKLIAEKLLEFETLDAKAIKS
                     LFEHGVMPEGTESNDYPSEKEAKTFEEAKRALEEKDAEKQAEEKRDFEEAKHELNEEN
                     KAEHDSDSQQSEDNHSDDQYK"
     gene            complement(5944..6489)
                     /gene="hpt"
                     /locus_tag="ACG8KR_001178"
     CDS             complement(5944..6489)
                     /gene="hpt"
                     /locus_tag="ACG8KR_001178"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HFQ0200561.1"
                     /translation="MLEKDIERILISQEEIQVRCKELGKELTEIYQNTNPLVVGVLKG
                     AVPFMADIVRSIDTYLELDFMDVSSYGNATVSSGEVKIVKDLDTNVEGRDLLIVEDII
                     DSGRTLAYLVDLFKYRKAKSVKIVTLLDKPEGRVVNIEADYVGFNVPNEFVVGYGLDY
                     AEAYRNLPYIGVLKPSVYQSN"
     gene            complement(6489..7934)
                     /gene="tilS"
                     /locus_tag="ACG8KR_001179"
     CDS             complement(6489..7934)
                     /gene="tilS"
                     /locus_tag="ACG8KR_001179"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HFQ0200562.1"
                     /translation="MNVEKGGKKAPLLQQSFIRHGNEQGFWQQGSRILAAVSGGVDSM
                     VLLHLLLQVQRQIGFSLGIVHINHQLREESAEEAAYLRSYCEEKNLPLYVSIWEDPAK
                     SAIETEARNFRYQEFTRVMEEGQYDTLMTAHHGDDQIETVLMKIVRGGQLNTFSGIKE
                     VQPFATGRLIRPLLSFSKEDLYTYAAESQLVYFEDQTNQLLDIQRNRLRHLVVPQLKQ
                     ENTQALRHFQQFSQQIQWADQVIQKYMGQLIEKEVEQWEEQFRFSAEMIEEMTDAERY
                     YFFYSFFDKAFSKTGIILKEQQMESILDQWQQKKSQWQLSIGNNWILKREYHFVVLSK
                     KEKQATSQTEDQRLLLIPEQGIYLNETEWIGLVKPENAGDIKATDSWSLFSHDIWIPA
                     DVQLYVEKRKAGDRIKLTENLTKKVSRYFIDQKVPNSQRQHAWIITDAKRNIYSLIPF
                     AFSYLSIREETDKIHYILLYKYQKEAIGRRT"
     gene            complement(8054..8524)
                     /locus_tag="ACG8KR_001180"
     CDS             complement(8054..8524)
                     /locus_tag="ACG8KR_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 domain-containing RNA-binding protein"
                     /protein_id="HFQ0200563.1"
                     /translation="MSIEVGAKLQGKVSGITNFGAFIDLGAGKTGLVHISEVSNGYVK
                     DIHDVLSVGDEVTVKVISVNDDGKIGLSIRKAQEQPAESGEESRPKKYQQKDPRDNRN
                     RPAQKKSFSRSQPSAQKEDFDSLMSSFLKDSDDRLSSLKRNTEGKRGGRGGRRS"
     gene            complement(8570..8995)
                     /locus_tag="ACG8KR_001181"
     CDS             complement(8570..8995)
                     /locus_tag="ACG8KR_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HFQ0200564.1"
                     /translation="MTKKSNKIAALDTEYAKKKYAEFQQQQRQLIFRRRRLAAIFVGA
                     LVIFAIIGVQIFHDMQRMHQLNELRVEANTEMKEVNADVDQLHQDVSLLKDDEYVAKL
                     ARSKFFYSKDGEKVYPILESNTDSSTNETDQSTSSTTAK"
     gene            complement(9062..9331)
                     /locus_tag="ACG8KR_001182"
     CDS             complement(9062..9331)
                     /locus_tag="ACG8KR_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HFQ0200565.1"
                     /translation="MRLDKFLKISRIIKRRSVAKEVADKGRIQINGKLAKSSSDVKVG
                     DILKVQFGNKTLEVKITELHESTKKEDAQKMYEIISENRTETLEK"
     gene            complement(9342..10937)
                     /locus_tag="ACG8KR_001183"
     CDS             complement(9342..10937)
                     /locus_tag="ACG8KR_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFQ0200566.1"
                     /translation="MDHKEMRRVMQGAFVLTIASFIAKVLSALYRIPLQNLVGDEGFY
                     VYQQVYPIYGIAMTLALSGLPQFISKYVAERKNPFERKQALQNLYPLVFWTGVFLWLF
                     VFAFSSWIAWMMGDIQLKPLIQVVSFTFLLMPGLSFYRGNFQGRFLMEPTAVSQVVEQ
                     FVRVFVIVCSAFAFRKLGWTVYQTGTAAMSGAVFGGLCAYGILHYYEQKIHGGALSFR
                     RFPLFKQPSKPLIRRFLVEGGLVSIYSGLLIFFQLIDSFFVKNALEVYGLSEYGAKIA
                     KGVYDRGQPLVQLGLVIATALSATFLPALTRHLTNRIYRQFLQTAKIYLRLTTALALA
                     ASLGLALLLPYINYALFKDYAGNAALVLFVFSIAFTAVIQAYQSIAQSKNSFRPSLKG
                     AGWGLLVKGLTTSFLTGLLGTAGASLSTLLGLGVTLWYFVRIETKELNAFWKERHFGR
                     KLMFSLLCMTVVLLLYYGVLNIVFGPVTHRSTAFFVSVIGVGIGASVFIYAVIRLRLF
                     TVREWLMLPFGKKILRLNKKIKK"
     gene            complement(10952..14473)
                     /gene="mfd"
                     /locus_tag="ACG8KR_001184"
     CDS             complement(10952..14473)
                     /gene="mfd"
                     /locus_tag="ACG8KR_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HFQ0200567.1"
                     /translation="MDIIQLVGKDPQVKEWVSNLEEKMPRQLITGLAGSAKTLLFARA
                     FKKTNKNMIILMPNLYYANQLAEDLQHVIPQDQIHLFPVDEVLSAEMAFSSPEARGER
                     VATLNALQEKKAGIYLLPVASLHKRLPNKKTWLQAQLNWHIGDEIDVENLPRQLTLMG
                     YVREEMVAKPGEFSIRGSIIDIYPLTTTYPVRVELFDVEIDSMRYFEAETQRSIEKTE
                     EIWVSPTSEQVYGPEDLEHGIEQMQKMLEKRLAATTEEADREFLQDYFGQLTSSWQAG
                     VPTEEAKFYSDLLYQESVTILDYFPSNSLLVVDDYQRIMETNREIEREAAEWQTQKIS
                     ELRVFSEQTFSADIQKIVQKEKFTTTFFSLFQKGMGNLRFQAIHNFQYRTMQQFFGQM
                     PLLKTEMDRWRKQKQTVVIFIPTKDRIRKAEQMLRDEDILVVETEKNALIRGQVQLVE
                     GALQAGFELPQEKIVAITEKEIFQKTTKKQTRRQTVTNAERLKSYNELKAGDYVVHAN
                     HGIGKYIGMETLEVDGVHQDYMTILYQNDDKLFIPVTQLNLIQKYVASEAKAPRINKL
                     GGSEWTKTKRKVSSKIEDIADDLIKLYAARESEKGYAFGPDDAYQKEFENAFPYSETD
                     DQLRSAAEIKRDMEKEKPMDRLLVGDVGYGKTEVALRAAFKAIKESKQVAFLVPTTIL
                     AQQHYETMLERFEGFPVNIGLLSRFRTRKQQKETIEQLRTGQVDIVVGTHRILSKDIE
                     FSDLGLLIIDEEQRFGVKHKERLKQLRAQVDVLTLTATPIPRTLHMSMLGVRDLSVIE
                     TPPANRYPIQTYVMEKNPGAIREAIHREMGRGGQVFYLYNRVETIEQKVEEIQELVPE
                     ARIGYAHGQMTEAQLENTLFEFIEGQYDVLVTTTIIETGVDIPNANTLFVENADYMGL
                     STLYQLRGRVGRSSRVAYAYFMYEQQKILNEVSEKRLQAIKDFTELGSGFKIAMRDLS
                     IRGAGNLLGAQQHGFIDAVGFDMYSQMLSEAVSRKQGKNSQVEKTTVEIDLGVDAYLP
                     ETYVADQRQKIEIYKRIRELDSQEMLDELEDDLLDRFGEYPEEVAHLLTIGQIKMDGD
                     RALLETIRKQEAHIVFTLSKVGTKRYSVEQLFEALTATKLKASLGVEKEQMVIRLTIP
                     NQMEEAVWLQEIQKFVKALREQKYLSA"
     gene            complement(14490..15050)
                     /gene="pth"
                     /locus_tag="ACG8KR_001185"
     CDS             complement(14490..15050)
                     /gene="pth"
                     /locus_tag="ACG8KR_001185"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HFQ0200568.1"
                     /translation="MKMIVGLGNPGTKYQYTKHNIGFMVVDKIAREHQATFKKNPFEA
                     EVAEFFHNGEKILLVKPQTFMNESGRAVGPLMTYFGIYPEELVVIYDDLDLAVGKIRL
                     RQKGSAGGHNGIKSIISHLNTNVFDRIKVGIGRPEGKKTVVQHVLSPFSKENQPLIEE
                     SMCQSVKAVEYLIEGHSFVDAMNRFN"
     gene            15403..16377
                     /locus_tag="ACG8KR_001186"
     CDS             15403..16377
                     /locus_tag="ACG8KR_001186"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004459 - L-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HFQ0200569.1"
                     /translation="MTAAAEKKDHQKVILVGDGAVGSSYAFALVTQNIAQEVGIVDIN
                     TAKTEGDAIDLSHALAFTSPKKIYSATYADAHDADLVVITAGAPQKPGETRLDLVNKN
                     LKINRDVVTQIVESGFNGIFLVAANPVDILTYSTWKFSGFPKERVIGSGTSLDSARFR
                     QALAELVDVDARNVHAYILGEHGDSEFPVWSHANVAGLQIYEWVKNNPEIDEEAMVNL
                     FFGVRDAAYTIIEKKGATFYGIAVALARITRAILDDENAVLPLSVFMNGEYGLNDIYI
                     GAPAVINRQGIQKVIEIPLSDSEQDRMAASAKQLKEILDEAFAKLDAE"
     gene            16547..17947
                     /locus_tag="ACG8KR_001187"
     CDS             16547..17947
                     /locus_tag="ACG8KR_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HFQ0200570.1"
                     /translation="MTRSSHRKKSKHVALLSVLGVLLVLISFYTYRSTYYTKHFLPNT
                     EINGINVSNLTVEKANEKLKEAYSDKKLAIKEDGKVWQEVPKSELGYKDDFTSELSPI
                     LNEQNGWTWGMTYVSAAEKQEIDPVSQNHQKLDTTVQTLTTKLTDLNKDRTPTTDATI
                     EKAGDSFVIKPEVNGNTIDVDAAVKQLKSAVNSGKDTIELTEFKEKPKVTSEDSSLKE
                     QLASMNAIANVKATYSINGETFQIPSSDIMSWLTYNDGKVDLDTEQVRQYVTDLGTKY
                     NTSTNDTKFKSTKRGEVTVPVGTYSWTIQTDSETEALKKAILAGQDFTRSPIVQGGTT
                     ADHPLIEDTYIEVDLENQHMWYYKDGKVALETDIVSGKPTTPTPAGVFYVWNKEEDAT
                     LKGTNDDGTPYESPVNYWMPIDWTGVGIHDSDWQPEYGGDLWKTRGSHGCINTPPSVM
                     KELFGMVEKGTPVLVF"
     gene            complement(18025..18747)
                     /locus_tag="ACG8KR_001188"
     CDS             complement(18025..18747)
                     /locus_tag="ACG8KR_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0200571.1"
                     /translation="MSGHSKWHNIQGRKNAQDAKRGKVFQKLSREIYMAAKAGGPDPS
                     MNPALRLVVDKAKAANMPNDNVERAIKKATSAVDETNYDEITYEGYGPGGVGILVHAL
                     TDNRNRTATNVRVAFTRNGGSLGETGSVSYLFDRKGYIAIERDGLTVDEETMFEDVLE
                     AGAEDLQTSPEVFEIYTAPEDFAAVRDELEKEFTLAQAELTMIPQTTIDLNEEQKEQL
                     ERLVDKLEDDDDVSEVFTAAEM"
     gene            complement(18826..19332)
                     /locus_tag="ACG8KR_001189"
     CDS             complement(18826..19332)
                     /locus_tag="ACG8KR_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFQ0200572.1"
                     /translation="MNSSKKQMKNGNIYLVIALIVVIILFYSSSQTYGQQSQVSRLES
                     WLPNQPFKDTLAGIRFNYGGSMVSIDHLGYAKFLEFFLRKGAHFGTYFILGGSLFLGV
                     FPKLKIWWLTAILAWLSATGYAGLDEFHQMMTGGRTPMFQDVMLDSIGALTAVILCVL
                     FAAFKKRL"
     gene            19455..21254
                     /locus_tag="ACG8KR_001190"
     CDS             19455..21254
                     /locus_tag="ACG8KR_001190"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HFQ0200573.1"
                     /translation="MSYSLNWNLDSIFSGGSHSDALNQRMKQLEDQMNEYYHRVTKWS
                     PSSDNAEQLNAILQLQETITNGFTQCSSYITALLSANVNDSDAKVLSGKLYAMLPRLQ
                     SAETVLSKKFAEISDNDWNQMLSQSSFETIAFRLNEIRRDGSQLLSEAEENIINTLSL
                     DGLNAWSSHYDTIVASISIPFEQDGQVVELSAGQAFNKMMGDPDPKVRETLFAAWEEA
                     WKEKAPLFTDTLNHLDGFRLSDYELHGVTDFLQKPLEYNRLKKETLSVMWDTIQKNKQ
                     PLVNYLTRKANLFGKEKMEWQDQDAPIILGDLKEKTFSFDEAAAFIIENFNKFSPKMA
                     KFAQSAFEKSWIEAEDRPGKRPGGYCTELPETKESRIFMTYSNSVNEVATLAHELGHA
                     FHSSTMWDLPSLNREYAMNVAETASTFAELIVADATLKVAKTKEEKINLLDTKLQNAL
                     AMFMNIHSRFIFENRFYEARQEGLVSEDKITEMMVEAQKEGYQGALATYHPYFWAAKL
                     HFFIDDVPFYNFPYTFGYLFSLGIYAYANQKGANFEEEYIHLLRDTASMTTEDLAKKH
                     LNVDLTQPDFWQAGIDMVTEDIQTFMELTEEFI"
BASE COUNT         5911 a         4665 c         3835 g         6860 t
ORIGIN      
        1 gaattcctta gacagctttt attcgattat ctcattgttg ggaatagcaa aacatcccga
       61 attgattgtg catcagttag caacattact aatctgtcga tcccgatgcc tagaccacca
      121 gttggcggca taccgtattc taaggcttcc aagaagtctt cgtctacacc gtgagcttcg
      181 tcatttccta gttcacgttc tttttcttgc gcttcgaatc tttctctttg atcaattgga
      241 tcgttcagtt ctgtaaaggc attagcaaat tcgcgcccca ctatgaatag ttcgaatcga
      301 tctgtgaatc gaggatcttc tggatttttc ttcgctaaag gcgaaacttc gactggatgt
      361 ccgtatacaa acgtcggttg tgtcaatgtt tcttcaacaa atgtttcaaa gaattcattg
      421 ataacatgtc cgacacccat agcttcggtt acttctacat tgtgtttttc ggctaaagct
      481 aatgcttctt ccaatgtcat ttcttgccag aagtctacac cagtttgttc tttgattgca
      541 tctaccatat ggatgcgttt aaagtcactt tctaagtcga cagcttgtcc atcatatgtg
      601 atttttgctg ttcctagtac tttttcagct gcgttacgga taataccttc tgtcagattc
      661 attacatcat tgaaatcagt atatgctgtg tatgcttcta acatcgtaaa ttctggatta
      721 tgtgtagtat caattccttc attacggaaa acgcggccga tttcataaac tttttccatt
      781 ccgccaacaa ttagtcgttt taaatgaagt tctagcgcaa tacgcaaata taaatcaatg
      841 tccagtgcat tatgatgcgt gataaatggg cgtgcggcag ctccacctgc ctcattgtgc
      901 aatactggtg tttctacttc aatatagcca ttgccatcta aatagcgacg gatttcgctg
      961 ataatttggc ttcgtttcat aaatcggtca aagctttcgc gattactgat taaatccaag
     1021 tagcgttgtc tgtagcgttg ttcaatattc gttaagccat ggtatttatc tggcagtgga
     1081 cgaagtgctt ttgacaaaat cgtgatttct gaagctttga tagaaacttc gcctgtgtct
     1141 gttttcatga tttgtccagt tacaccaaag aaatctccaa ggtctgcatg tttgaatact
     1201 tcataggcct catcacctac ttggtctttt cttacataaa tttgaatttg gccttcacga
     1261 tcttgtagat gcgcaaatcc agttttccct tttcctcggc gagtcatcat acgaccagct
     1321 acactagcgg tcaatcccat ttctgctagt tcttcttttg ttcttggatc aaatagttca
     1381 tgcaattctt ctgagttgtg cgtgcgttca aatcgttttc caaatggatc gattccatct
     1441 tcacgcaatt gttccatttt ttgacgacgt acaagcattt gatcatttaa atcttctact
     1501 tgatgttgtt gttcctgtgt cacaaaagtt cctccattct catgttaatc tctttcctat
     1561 aatgccaatg aaacacaaaa aaagcaagcc gaacttggaa tttcggcttg aaaattcacg
     1621 aaaaacttag aaatcacaac gtttgtttca atagtacttt ttccgctttt tctacaaatt
     1681 gatccagcag gtcattgatt tcttgctgtt tttctgtctg attgattgct actttcactt
     1741 tcgctgctcg aggaatccct tttaaataat aagccgcatg tgttctgaat tctctacaag
     1801 ctactttttc tcctttgaga tctgctaagc gttgtaaatg aagtttagct gtagcaattt
     1861 tttcggctgg tgttggttca ggaatcagtt ctcctgtttc gagataatgc tgtgtgcggt
     1921 gaatcatcca tggattgccc agcgctgcac gaccaatcat cacaccgtct gcaccgacat
     1981 agtcaagcat ccattttgca tcttctggcg ttttgacatc cccgttcccc ataaatggga
     2041 tcgtcaaatg ttttttcact tctttcaata cttcccagtc cgcttttcct tcatacatct
     2101 gaacccgtgt acgtccatgc atagcgatgg cacttgctcc agctgattgt gctgctaatg
     2161 cattttctac agcaaagacg tgctcttcat cccaaccaat acgcatcttg attgttactg
     2221 ggatatttac tgctgaagaa acagcttgga ccatttcgta tactttctca ggatctaaaa
     2281 gccattttgc tcctgcttct gctttgatta ctttatttac tggacagccc atattgatat
     2341 ctattatatc ggcagctgtg ttttcttcga caaattgagc agcttcaact agactatctt
     2401 tatttccgcc aaatatctgt aaacttaatg gatgttctgt ttcttcaatg tggagcatct
     2461 ctaaagtttt tttgtttctg aaatgaatac cttggtcact gatcatttca catacgacta
     2521 agcccgcacc aaattctttg acagtgactc gaaatgctga atttgttatt ccagccatcg
     2581 gtgcaacaac gacacgatta gggatttcca cattgccaat tttccatgtg tgttccactt
     2641 aaatccctcc taaggcttct ttcttcaatc catctaattc ttcttcgcta tacttgtatt
     2701 tgttattaca gaaagaacaa accgcttctg caccgtgatc ttcgtctatc attgcttgga
     2761 tttcttctgc acctaacgcg ataattgctt ctgcaaattt ttcttttgaa cagtcacatt
     2821 tgaattgaac aggcattttt tccaaaatgt tcaattcttc tgtaccaagc aatcctttca
     2881 atatttcttc tggtgtctgt ccttcatcta agagggtaga gattcttggt gtcgttttta
     2941 attgttcttc gatcttgctg attgtttctt catcggcacc tggcatgatt tggatcatga
     3001 atcctcccgc cgtcttgatg ctgtcgtcag tattgaccaa tacactcagt ccaacagctg
     3061 aagggatttg ttctgagata gcaagatagt aagtaaaatc ttcagcgatt tctccagaaa
     3121 caatcggcgt ttgcccagaa aaaggttctt ttaatcctaa gtcttttgtg acagtaaaca
     3181 ttccttgtgt tccaactgct ccccgcacat cgattttacc ttcactattt agaggaagac
     3241 tgagatgcgg atttttgatg taacctttca catctccctt tccatttgca tctactacaa
     3301 ttcctgttgc tggaccatcg ccttggattc gaacagttag tttgtcttct cctttgagcg
     3361 tcgcaccaag tagcagacta ccgaccaatg tccgtcccaa agcagctgag gaggtgtgcc
     3421 aagtatcatg acgtctttgt gcctccgcta cggtatttgt tgcttgaacg gcgtatgctc
     3481 ggacgaatcc gtcgtgagca agtgccttaa ccaaataatc tttcatttgt tatgtactcc
     3541 tttttccgtt tttaaactca tagtgaatca tactagcaac tactgctttt ttcaactttg
     3601 gaatgcttct tttaaaaata attcagaatc atctagaaaa caggacatca ccctatttac
     3661 aacttaaata aagaaatctc ccgaaaaccg gtttttgttc cggttttcgg gagtcatatc
     3721 agatcaattg acctatattt ttttatttgt attgatcgtc tgaatgatta tcttctgatt
     3781 gttgagaatc tgaatcatgt tctgctttat tttcctcatt cagttcatgt tttgcttctt
     3841 caaaatcacg tttttcttcc gcttgttttt cagcgtcttt ttcttccaaa gcacgttttg
     3901 cttcttcaaa agttttcgct tctttttcgc ttggataatc atttgactca gtaccttcag
     3961 gcatcacacc gtgttcaaac aatgacttga ttgctttggc atccaatgtt tcgaattcca
     4021 aaagtttttc agcaatcagt ttatgctgtg cacgatgagc ttcgatgatt tctcgagctt
     4081 tatcatgagc ttccatcaag atttttcgta cttcttgatc aatctcgaaa gcgacttgtt
     4141 ccgagtaagc ttttgtttga ccatagtcgc ggccaacaaa cacttgatgg tttccttcat
     4201 attgaacagg acctaaacga tcactcattc catattctgt taccatgctt cgtgctagag
     4261 ctgtcgcttg ctcaaagtca ttggaagcac cagttgattg gacattgaag atgatttctt
     4321 cagctgtacg tccaccaagt aagcctacga tttgttcaaa catatcttct ttcgtcatca
     4381 agaactgatc ttcttttggt aaagcgatca tatatccgcc ggcgcgtccg cgagggatga
     4441 tcgttacttt gtggacgacc cgagcgcgac tcaatactaa tccgacaatc gtatgaccag
     4501 cttcgtgata agcaaccatt tcgcgttctt ttttgctgat tacgcgatct tttttagctg
     4561 gacctgcaat cacgcgatct tccgcttcat cgatatcaga agcatcgatt ttctttttat
     4621 ttcttctagc agctactaac gctgcttcat tcaagacatt ttctaaatct gctccggcaa
     4681 aacctggtgt ctgttgagcc acaactttca agtcaacgtc atctgccatc ggtttattcc
     4741 gtgcatggac tcgcaagatt gcttcacgac cttttacatc tggacgtcca actaaaatct
     4801 ggcggtcaaa acggcctgga cgtagtaacg ctgggtctag tacatcagaa cggttggttg
     4861 cagcaatgac gattactcct tcatttccgt cgaagccatc catttctaca agcaattggt
     4921 tcaatgtttg ttcgcgttca tcatgtccgc cgcccatacc ggcaccacgt tgacgaccta
     4981 ccgcatcaat ttcatcaatg aaaatgatag ctggtgcgtt tttcttcgct gtttcaaaca
     5041 aatcacgaac acgacttgct ccaacaccga caaacatttc tacgaaatca gaacccgaga
     5101 tcgagtaaaa cggtactcca gcttctccgg caacagcttt tgctaataag gttttacctg
     5161 ttcctggagg tccttctaaa agtacacctg ccgggatacg ggcacctaat tcaacaaatc
     5221 ggcgaggatc tttcagaaat tcaacaactt caactagttc ttgtttttct tcttccgcac
     5281 ccgctacatc agaaaaacgt acgcgattag cttttttgtc tgcttctttg gcttttgatt
     5341 taccaaagtt catgacacgg ccgccaccgc caccgccgcc gccttgttgt cccatcatca
     5401 tgtaaaagaa aaagatgatg attacgattg gcaagaagct aagcaatagg gaaacccata
     5461 taccgctgct tgattcttct tttactgttg tagcaacttt gttttctttt gccagttctg
     5521 ttacttggct caatgttgta tcatttggca aaatgattgt tgtgaaatgg gtagtagtcg
     5581 tatctgttga acctaagata gctaacccac cgctgttagc gacggtctgc tgttctttgt
     5641 attcaccagt gatcttatat acaccatttg caggctgcac ttcaaattct ttgatcttgc
     5701 ctgcttgtag ttgttctgtg aattttgagt actcgatatc tgacgatcgc tggccgttat
     5761 taccaaaaat gaaataaacg accattacca tcgctaagat tacgaggaca taatacaagg
     5821 catttttcat catgccgttg ttctttttgt tcatagttgc cctccctaag cgtcaattta
     5881 atttaacagt tttttatcaa tcctgaccat tctattgtac cataatttaa ttgagataca
     5941 gcattaattt gattgataaa cactaggttt caatacaccg atgtacggaa ggttacgata
     6001 tgcttctgca taatccaagc cataaccgac cacaaattca tttggtacgt tgaagccaac
     6061 ataatctgct tcgatattta ctactcttcc ttcaggtttg tcaagtagtg taacaatttt
     6121 gactgatttt gctttgcggt atttgaaaag atccactaaa taagcaagtg ttcttccgct
     6181 gtcaatgata tcttcaacga tcaacagatc gcgaccttct acattcgtat ctaagtcttt
     6241 gacgattttt acttcccctg acgagactgt tgcattcccg tagcttgaaa catccatgaa
     6301 atccaattcc aaatacgtat cgatagaacg aacgatatct gccatgaatg gtaccgctcc
     6361 ttttaagaca cctactacta ggggattggt gttttggtaa atttctgtta actctttgcc
     6421 tagttctttg caacgaactt gtatctcttc ttgtgagatc aaaattctct caatatcctt
     6481 ttccaacatc aggttctcct tccaattgct tctttttgat atttgtaaag aagtatgtag
     6541 tgtattttat cagtttcttc acgaatactc aaatatgaaa aggcaaaagg tatcaagcta
     6601 tatatatttc ttttggcatc tgtaatgatc caagcatgct gcctttgtga attaggtact
     6661 ttctgatcga taaaatatct agatactttt tttgtgagat tctctgttaa tttaatgcgg
     6721 tctcctgctt ttcttttttc gacatacagt tgcacatcag caggaatcca aatgtcatga
     6781 gaaaatagag accatgaatc tgttgctttg atgtcccctg cattttcagg tttgaccaga
     6841 ccaatccatt ctgtttcatt caaatatatt ccttgttctg gaatcagcaa caagcgctgg
     6901 tcttctgtct ggctagttgc ttgtttttct tttttagaga gtacaacaaa atgatactct
     6961 cttttcagta tccaattatt gccaatactg agttgccatt ggcttttttt ctgttgccac
     7021 tggtctaaaa tcgactccat ttgttgttct ttcaaaataa ttcctgtttt tgagaatgct
     7081 ttgtcgaaaa aggagtaaaa aaaatagtat cgttcagcat ctgtcatttc ttcaatcatt
     7141 tctgcagaaa atcgaaattg ttcctcccac tgttcgactt ccttttcgat caactgtccc
     7201 atatattttt gaatgacttg gtctgcccat tggatctgtt gcgaaaattg ctggaaatgt
     7261 ctcaacgcct gtgtattttc ttgtttcaat tgaggaacta ccaaatgacg aagccgattt
     7321 ctctggatat ctaacaattg gtttgtctga tcctcaaagt aaacaagctg cgattccgct
     7381 gcataagtat ataggtcttc tttgctaaaa ctcagcaacg gacgaatcag tctgcccgta
     7441 gcaaatggtt gtacttcttt gattccagaa aaagtattta actgtccccc acgaacgatt
     7501 ttcattaaca ccgtctcgat ttgatcatct ccgtgatgag cagtcatcaa cgtgtcatat
     7561 tgtccttctt ccatcacgcg agtgaattct tgataacgaa agttacgagc ttctgtttct
     7621 atggcggatt tggctggatc ttcccaaata cttacatata aagggagatt cttctcttca
     7681 caatatgagc gaagataggc agcttcttct gctgattctt ctcttaattg gtgattgatg
     7741 tggacgatac ctaaagaaaa accgatttgg cgttgcactt gtaaaagtaa atggagcaaa
     7801 accatcgaat caacaccacc tgataccgct gctaaaattc tcgatccttg ttgccagaat
     7861 ccttgttcat tcccgtgccg aataaatgac tgctgtaata gcggtgcttt tttcccgcct
     7921 ttttcaacgt tcatcctttc cacacccttt cttcctaaaa aaaagagagc gggaaaacgt
     7981 ctaaaacgac aattaacctg ccctctgttg atactatgac ccaaatagtc gcactatcat
     8041 tatttgcggc tttttagctg cgacgtccgc cacggccacc gcgctttcct tctgtgtttc
     8101 gcttcaatga agataagcgg tcatcgctat ctttcaaaaa tgaactcatc aatgaatcga
     8161 agtcttcttt ttgagctgaa ggttgactgc gagaaaaaga ttttttctgt gctggacgat
     8221 ttcggttgtc acggggatct ttttgttgat attttttagg acggctttct tctccagatt
     8281 cagcaggttg ttcctgcgct ttccggatag atagtccgat ttttccatca tcatttactg
     8341 aaataacttt aactgttact tcatctccta cacttaaaac atcatggatg tcttttacat
     8401 agccatttga cacttcgcta atatgaacta gacccgtttt tccagcacct agatcaataa
     8461 acgcaccaaa atttgtgatt cctgatactt ttccttgcag ttttgctcct acctcgattg
     8521 acataaaaaa aatgttcctc ctaattattt ccctttttta tattcctgct tattttgcag
     8581 ttgtagaact agttgattga tctgtttcat ttgtcgagct gtctgtattt gattctaata
     8641 taggataaac tttttcaccg tcttttgagt aaaagaattt gcttctggca agtttagcta
     8701 catactcatc atctttcagc aatgaaacat cttggtgcag ctgatcaacg tctgcgttga
     8761 cttctttcat ttcagtattt gcttcgactc tcaattcgtt caattgatgc atcctttgca
     8821 tgtcatggaa aatctgcaca cctattatcg cgaaaatgac caaagcacca acaaaaattg
     8881 cagccagtct gcgtcttcga aaaatcagtt gacgttgttg ttgctgaaat tcagcatact
     8941 ttttctttgc atattccgta tctagcgcag cgattttatt gctttttttc gtcacttgga
     9001 ttcgctccaa tctttttgat tgtctttttc attataccaa tatcgtgaaa gcttgtctaa
     9061 tctatttctc tagtgtttct gtgcgatttt cagaaataat ttcgtacatt ttttgtgcat
     9121 cttctttttt ggttgattca tgtaattccg taattttcac ttccaacgtt ttgttgccaa
     9181 actggacttt caaaatatcg cctactttga catcactgga agatttcgcc aatttaccgt
     9241 tgatttgaat tcttccttta tcagctactt cttttgctac tgatcgtctt ttgatgattc
     9301 gggaaatttt taaaaattta tctaatcgca tatcttattc ctcacttttt aattttttta
     9361 tttaatcgca ggattttctt accaaaaggc aacattagcc attctcgtac tgtaaacaga
     9421 cgaagacgaa tcactgcata aatgaacaca gaagccccga tcccaactcc tatcacgctg
     9481 acaaaaaaag cagtggaacg atgagttaca ggaccaaata ctatattcaa tacaccataa
     9541 tacagcagca gtactactgt catacaaaga aggctgaaca tcaacttccg tccaaaatgc
     9601 cgttctttcc aaaaagcatt tagctctttt gtttcaattc gaacaaaata ccaaagcgtc
     9661 acacctaatc ctaaaagggt agacaggctt gcacctgctg ttcctaataa accggtcaga
     9721 aaagaagtag tcaatccttt taccaatagt ccccatcctg cgccttttaa agaagggcga
     9781 aacgagttct tgctttgagc aatactttga taagcttgga tgactgcagt gaaagcaatg
     9841 gaaaaaacaa ataatacaag cgctgcattt cccgcataat ctttaaacaa cgcataattg
     9901 atataaggga gcagcaaggc taatcccaaa gacgcagcta atgctaaagc ggtcgtcaat
     9961 ctaagataga ttttggctgt ctgtaaaaac tgacgataga tccggttggt caaatgacga
    10021 gtcaacgcag gcaggaatgt cgcacttaat gcagtggcaa tcactaaacc taattgtact
    10081 aaaggctgac ctcgatcata gacccctttt gctatcttcg caccgtattc tgacagtccg
    10141 taaacttcca aagcattttt tacaaagaaa gagtcgatca gctggaaaaa gataagcaat
    10201 ccactataaa tcgaaaccag tccgccttct accagaaaac gccggatcaa gggctttgac
    10261 ggctgtttga acaatggaaa ccgtcggaag cttaacgctc ctccatgaat cttttgttca
    10321 tagtagtgaa ggatcccata tgcgcataaa ccgccaaaaa cagctccact cattgctgcc
    10381 gtccctgttt ggtacaccgt ccatccaagc tttctaaaag caaacgcact acagacaatc
    10441 acaaaaacac gaacgaactg ctcaactact tgagaaacag cagttggttc catcaagaat
    10501 cttccttgga aattccctcg ataaaaagat agaccaggca tcagcaaaaa ggtaaaggaa
    10561 actacttgga tcagcggttt caattgtatg tctcccatca tccaagcaat ccagctgcta
    10621 aatgcaaaga caaacaacca caaaaagaca cctgtccaaa atactaaggg ataaagattt
    10681 tgcaaagctt gctttcgctc gaaaggattc tttctttctg caacgtactt tgatatgaac
    10741 tgcggaagac cggataaagc taaagtcatt gctatcccat aaattggata aacttgctga
    10801 taaacataaa aaccttcgtc gccgactaag ttttggaggg gaattcggta aagggcactt
    10861 aatacttttg ctatgaaaga agcaatcgtc aatacaaatg ctccttgcat gacgcgccgc
    10921 atttctttat gatccaaatc attgctcctt cctacgctga cagatatttt tgctcacgca
    10981 acgctttgac aaacttttgg atttcctgaa gccatactgc ttcttccatt tggttgggga
    11041 tcgtcaagcg tatcaccatc tgttcttttt caacgcctag actggctttt agttttgtcg
    11101 ctgtcagtgc ttcgaataac tgttccacac tatagcgttt tgttccaact ttacttagag
    11161 taaagactat atgcgcctcc tgtttcctta tcgtttctag gagcgctcga tcgccatcca
    11221 ttttgatctg cccaattgtc aagagatgtg ctacttcttc tggatattct ccaaaacggt
    11281 cgagcagatc atcttctagt tcatctagca tttcttgcga atcaagttca cgaattcgtt
    11341 tatagatctc gattttctgc cgttgatctg ccacgtatgt ttcaggtaga tacgcatcga
    11401 caccgaggtc gatctcaaca gtggtcttct ccacttgact attctttcct tgtttacgag
    11461 aaacagcttc gcttagcatt tgagaataca tatcgaaacc gactgcatca ataaatccat
    11521 gctgctgagc acctaagaga ttacctgcac cgcgaatgga aagatcccgc atcgcaatct
    11581 tgaatccaga tcccagttct gtaaaatctt tgattgcctg aagacgtttt tcactgactt
    11641 cgttaaggat cttctgctgc tcatacataa agtaagcata tgcgacacgg ctgcttcttc
    11701 ctacccgccc tcgcaattgg tataacgtgg ataatcccat ataatcagcg ttctcaacga
    11761 acaacgtatt tgcgttcggg atatctactc ctgtttcaat gatggttgta gtgacaagaa
    11821 catcatattg accttcaata aattcaaaaa gcgtattttc taattgcgcc tctgtcattt
    11881 gcccatgagc ataaccgatc cgtgcttccg gaacgagttc ttggatttct tctacttttt
    11941 gttcgatcgt ttctacccga ttatataaat agaatacttg tccacctcgt cccatttctc
    12001 gatggattgc ttcgcgaata gccccaggat ttttttccat aacatacgtt tggatcggat
    12061 aacgattcgc aggcggtgtt tcgattactg aaagatcccg cacgcctaac atcgacatat
    12121 gcaaagttct cggaatcggt gttgcagtca gagtcaaaac atccacttgt gcacgtaact
    12181 gtttcaatcg ttccttatgt tttacaccaa aacgttgttc ttcgtcaatg atcagtagac
    12241 ccaaatcgct aaattcaata tcttttgaca aaattcggtg ggttcctaca acaatatcaa
    12301 cttgacctgt acgcagttgt tcgatggttt ctttttgttg ctttcttgtt ctgaaacgac
    12361 ttaatagccc gatatttacg gggaaacctt caaatctttc cagcatcgtt tcatagtgct
    12421 gctgtgccaa gatcgttgtt ggaactaaaa aggctacttg tttgctttcc tttatcgctt
    12481 taaaggccgc tcgaagcgct acttctgttt tcccataacc aacatcacct acgagtagtc
    12541 gatccattgg tttttctttt tccatatccc gtttgatctc cgctgcactt ctcaactggt
    12601 catcagtttc agagtagggg aaggcatttt cgaattcttt ttggtaggcg tcatcaggtc
    12661 caaaagcata tcctttttct gactctcttg cggcatataa tttgattaga tcgtcagcga
    12721 tatcctcgat ttttgaagag acttttcgct tggttttcgt ccattcactt ccgcctagct
    12781 tattgatgcg aggcgctttg gcttcagagg cgacatattt ttggatcaga ttcagttgag
    12841 taacaggaat aaacagtttg tcgtcgtttt gatacaaaat agtcatatag tcttggtgaa
    12901 cgccatctac ttctagcgtt tccatcccaa tgtatttccc gataccatga ttggcatgga
    12961 caacgtaatc tcctgctttt aactcattat agcttttcaa tcgctctgcg tttgttactg
    13021 tttgacgtct cgtttgtttt ttcgtcgttt tttggaaaat ttctttttct gtgatagcta
    13081 ctattttttc ttgaggaagt tcaaaaccag cctgcaaagc tccttcaact aattgtacct
    13141 gtcctctgat caatgcattt ttttctgttt ctactactaa tatgtcttca tctcgaagca
    13201 tttgttctgc tttgcgtatt ctgtcctttg tcggaataaa gatcacaaca gtctgttttt
    13261 gcttgcgcca acggtccatt tctgttttca gcaatggcat ttgtccgaag aactgttgca
    13321 tcgtccgata ttgaaaatta tggattgctt ggaaacgaag atttcccatc cctttttgga
    13381 agagtgagaa aaaggttgtg gtgaactttt ccttctgcac gattttttgt atatctgctg
    13441 aaaatgtctg ctcagaaaac acccgcaatt cgctgatttt ttgagtctgc cattctgccg
    13501 cttccctttc gatttcccga tttgtttcca tgattcgctg ataatcatca acgactagta
    13561 aactatttga agggaaataa tccaagatcg tcacagattc ttggtaaaga agatctgagt
    13621 agaattttgc ttcttctgtc gggacacctg cttgccagct tgaagtcagc tgaccgaagt
    13681 aatcttgcaa aaactcgcgg tctgcttctt ctgtggtcgc agcaagccgt ttttctagca
    13741 ttttttgcat ttgctctatt ccatgttcta gatcctctgg tccgtagact tgttcactcg
    13801 ttggggaaac ccagatttct tccgtttttt cgattgagcg ttgtgtttct gcttcaaaat
    13861 accgcataga atctatctct acatcaaata gttctacacg aacaggatag gttgtcgtta
    13921 acgggtaaat atcgataatg cttcctcgaa tactgaattc tcccggtttt gcgaccatct
    13981 cttctcgtac atagcccatc aaagtcaatt ggcgaggaag attttccacg tctatctcat
    14041 caccaatatg ccaattcaat tgcgcctgta gccaagtttt cttattcggc agtcgtttgt
    14101 gcaaagatgc gactggcaac aaatagatac ctgctttttt ttcttgaagt gcgttaagcg
    14161 ttgcaactct ctcgcctcgc gcttctggag aagaaaaggc catttcagca gaaagcactt
    14221 catccactgg aaataaatgg atctgatctt gcggaatcac atgttgcaga tcttctgcta
    14281 actgattcgc ataatacaaa tttggcatca gaataatcat gtttttattt gttttcttaa
    14341 acgctcgagc aaatagaagt gttttcgctg agcccgctaa tcctgtgatt agttgtctag
    14401 gcattttttc ttctaaattg ctgacccatt cttttacttg cggatctttt ccgactaatt
    14461 gaataatatc cattcctttt cctccttcct caattgaatc gattcatcgc atccacgaac
    14521 gaatgccctt ctatgagata ttcgactgct ttaacagatt gacacatgct ttcttctatc
    14581 aaaggctgat tttctttgct gaaaggacta agcacatgct ggactactgt ttttttccct
    14641 tctggcctgc cgattcccac cttgattcga tcaaagacat tcgtattcaa atgggaaata
    14701 atacttttga taccgttatg cccgccggca cttccctttt gacggagccg gatttttccc
    14761 actgccagat ccaaatcatc gtagattacg actaattcct ctggataaat cccaaaataa
    14821 gtcattagtg ggccgactgc acgaccagat tcattcatga atgtttgtgg tttgactaaa
    14881 aggatctttt ctccattatg aaaaaattcg gcaacttctg cttcgaacgg attttttttg
    14941 aaagtagctt gatgctcgcg tgcaatttta tccacgacca taaagccgat attgtgtttt
    15001 gtatattggt atttggtacc tgggtttcct aacccgacga tcattttcat ttttgttttc
    15061 cacctaattc taaaaagcag cacaaaaggc tggactgcta attagcggtc cagtccatcg
    15121 agcttttttc ttttttatac ttgtcaatgc atagtataac acaaaacaag gcgtttcggc
    15181 taaaagagaa tcgttaataa atgataaaga aattaaaaat ttcttcaaag tttaatgaaa
    15241 taattgatat ataaaaatag tgaaataacg cacatgtaag cttttacata taatacaacc
    15301 ttgttatctg ttatatttta tagccaaata gcgtgacatg gggcttgaga aatgttagca
    15361 taagcatggg aaaaaacaaa tcaatggaag gattgatact aaatgactgc agctgcagaa
    15421 aaaaaagatc atcaaaaagt tattcttgtt ggagacggcg ccgtaggttc tagctatgcc
    15481 tttgctctag ttactcaaaa tatcgctcaa gaagtaggta ttgtggatat caatactgct
    15541 aaaactgaag gagacgcaat tgacttgtct cacgctttgg catttacttc acctaaaaaa
    15601 atctattctg ctacttacgc agacgcacat gatgctgact tagttgttat tacagctggc
    15661 gcaccacaaa aaccaggtga aactcgttta gacttagtaa acaaaaacct aaaaatcaac
    15721 cgcgatgttg tcacacaaat cgttgaatca ggatttaacg gtatcttttt agttgccgct
    15781 aacccagtag atattttgac ttactcaact tggaaattct caggtttccc taaagaacgc
    15841 gtaatcggtt caggtacctc tcttgactca gctcgtttcc gtcaagcact tgcggaatta
    15901 gttgacgttg atgcacgtaa cgtccatgct tacatcttag gtgaacacgg agattcagaa
    15961 ttcccagttt ggtcacatgc aaatgttgcc ggcttgcaaa tctatgaatg ggtaaaaaac
    16021 aacccagaaa tcgatgaaga agctatggtt aacctattct ttggcgtacg tgacgctgct
    16081 tacacaatta tcgagaaaaa aggcgctact ttctatggta tcgcagtagc attagcacgt
    16141 atcactcgtg ccatcttaga tgatgaaaat gctgttcttc ctttatcagt attcatgaat
    16201 ggcgaatatg gattgaacga catctacatt ggtgcacctg ctgttatcaa ccgtcaaggt
    16261 atccaaaaag taatcgaaat tcctttaagt gacagcgagc aagaccgtat ggctgcttct
    16321 gctaagcaat taaaagaaat tctagatgaa gcttttgcta aactagacgc tgaataatct
    16381 ttgaacatga cggaaaacat ctattcggtg ttttccgttt ttttatttta tcacccttat
    16441 ggtttctaaa ttttttcata aagtagagat aaccttcgtc ttttcctcta aattatgcta
    16501 tcatgagacc gttccaccct aaacttttac ttaaggagtt gtcgatatga caagatcttc
    16561 gcatcgaaaa aaatcgaaac atgttgcttt gctttcagtt cttggtgttc tcttagtgct
    16621 gatcagcttt tatacctatc gcagtacata ctacacaaag cattttttgc cgaatacaga
    16681 aataaatgga ataaatgtca gcaatctaac agtcgaaaaa gcaaatgaaa aattaaaaga
    16741 agcttattct gataaaaaac tggctattaa agaagatgga aaagtctggc aggaagtacc
    16801 aaaatccgag ctcggctaca aagatgattt cacttcggag ctttctccga tcctaaatga
    16861 acaaaacgga tggacttggg gaatgactta tgtctcagcg gcagaaaaac aagaaataga
    16921 tcctgtcagt caaaaccacc aaaaactaga cactaccgtt cagacattga caactaagct
    16981 gactgatttg aacaaagacc ggacacctac tactgatgca acgatcgaaa aagcaggtga
    17041 ctctttcgtt atcaagcctg aagtcaatgg aaatacgatc gacgtcgatg ctgcggtcaa
    17101 acaattaaaa agtgcggtaa actctggtaa agatacaatt gaattgactg aattcaaaga
    17161 aaaaccaaaa gtaacatctg aagacagctc actgaaagaa caactcgcat ccatgaatgc
    17221 tatcgcaaat gtaaaagcta cttacagtat aaacggtgag actttccaga tcccatcaag
    17281 tgatattatg agttggctga cctacaatga tggaaaagtc gatttagata cagaacaagt
    17341 acgtcaatat gtaacagatt taggcacaaa gtataacaca agtacgaatg acacaaaatt
    17401 taaaagtaca aaaagaggag aagttacggt cccagttggc acttacagtt ggacgatcca
    17461 aacagacagt gagacagaag cattgaaaaa agcgattctg gcaggccaag attttactcg
    17521 ttcacccatt gtgcaaggtg gcacaaccgc tgatcatcca ttgatcgaag atacgtatat
    17581 cgaagttgat ctggagaacc agcatatgtg gtattacaaa gacggaaaag tggcccttga
    17641 aacagatatc gtttccggaa agccaactac acctacacct gctggagtct tctatgtttg
    17701 gaataaagaa gaagatgcaa ctttaaaagg aacaaatgac gatggaacac cttacgagag
    17761 tccggtaaac tactggatgc cgattgactg gacaggtgtt ggtatccacg attcagactg
    17821 gcaacctgaa tacggtggag acttatggaa aacccgcggt tcacacggct gcatcaacac
    17881 cccaccaagt gtaatgaagg aattattcgg gatggtcgaa aaaggaacgc ctgtattggt
    17941 cttctaacaa aaataatata taaaaagaaa gcacccgtac gattggtaca ggtgctttct
    18001 ttttattatc taacaatgtg ctacttacat ttcagcagca gtaaagactt ctgaaacatc
    18061 atcatcgtct tctagcttat ctactagacg ttccaattgc tctttttgtt cctcatttaa
    18121 atcaatcgtc gtttgtggaa tcatcgtcaa ttctgcttgc gccaaagtga actctttttc
    18181 caactcatct cgtacagcag caaaatcttc tggtgcagta taaatctcga atacttcagg
    18241 agaagtttgc agatcttctg cccccgcctc taatacatcc tcaaacatcg tctcttcatc
    18301 aacagtcaac ccatcgcgtt caatagcgat atatcctttg cggtcaaaca agtaactgac
    18361 tgaacccgtt tctcccagtg atccaccgtt tcgagtaaaa gctactcgga catttgtcgc
    18421 tgtccgatta cggttatctg tcaatgcatg gactaagatt cctactccac ccggaccata
    18481 tccttcatac gtgatttcat cgtaattcgt ttcatctacg gcactcgtag cctttttaat
    18541 cgcccgttcc acattgtcgt taggcatatt tgcagctttt gctttgtcca caacaagccg
    18601 caaagctgga ttcatagatg gatcaggacc gcctgcttta gctgccatat aaatttcacg
    18661 agaaagtttc tgaaagactt tccctcgttt tgcatcttgg gcattttttc ttccttggat
    18721 attatgccat ttactatgtc ctgacatgat ttttcctccc ttgttctttt gagaatacac
    18781 ttctattcta gtactatttc acaaaaaaac aaagccacag ccaatctata aacgcttctt
    18841 gaatgccgca aaaagcacac ataatatcac agcagtcaga gcaccgatcg aatccaacat
    18901 gacatcttgg aacataggtg ttcgtccacc ggtcatcatc tgatggaatt cgtccaaacc
    18961 tgcgtaccct gttgctgaaa gccaagcaag gatcgctgtc agccaccaaa tttttagctt
    19021 ggggaaaaca cctaaaaaca agctgcctcc caaaataaaa taggtaccga aatgtgcgcc
    19081 cttccttaaa aagaactcta aaaatttggc gtagcccaga tggtcgatac tcaccatact
    19141 tccaccataa ttaaaacgta tacccgctaa tgtgtcttta aatggctgat taggcagcca
    19201 gctttctaat cgcgaaactt gtgattgctg tccgtaagtt tgagaagaac tgtaaaataa
    19261 gattataacg actatcagtg cgattactaa ataaatattt ccgtttttca tttgtttttt
    19321 tgaagaattc ataagtcacc tctgtatttt atcttagact ttttttgaag aaacaacaat
    19381 cattttcata gagacttctt ccataaaaaa gtgctatact taattttgta ttacaatatg
    19441 aagggagttt tttcatgagt tattcattaa attggaatct agattctatt ttttccggag
    19501 gcagtcattc tgatgctttg aaccaacgga tgaagcaatt ggaagaccaa atgaacgagt
    19561 attatcaccg cgtaacaaaa tggtcaccta gttctgacaa tgcagagcag ttaaacgcta
    19621 ttcttcaatt gcaagaaacg attacgaatg gttttaccca atgcagcagc tatattactg
    19681 cattgctttc tgccaatgtc aatgattcag atgcaaaggt cttatcaggc aagctttacg
    19741 caatgctgcc tcgtctgcaa tcagccgaaa ctgtattgtc aaagaaattt gcagaaatat
    19801 ctgacaatga ttggaatcag atgctttctc aatcttcgtt tgaaacaatt gcttttcgtt
    19861 taaacgagat tcgtcgtgat ggctcacaat tattatcaga agctgaagaa aacattataa
    19921 atacgctttc tttagatggt ctaaatgctt ggagcagtca ttatgatacg attgtagcca
    19981 gcatttcgat tcccttcgaa caagatggac aagtagtcga attatctgct ggacaagcct
    20041 tcaataagat gatgggcgat ccagatccaa aagtcagaga aacacttttc gcagcatggg
    20101 aagaagcctg gaaagaaaag gcacccctct tcacggatac tttgaatcat ttagatggtt
    20161 ttagactttc tgactatgaa cttcatggtg tcacagattt tctacaaaaa ccattagagt
    20221 acaatcggtt gaagaaagaa acattgtctg tcatgtggga tacaatccag aaaaacaaac
    20281 aaccccttgt taattacttg actagaaaag ctaatctttt cggtaaagaa aaaatggaat
    20341 ggcaagatca agatgcacct attattttag gcgatttgaa agaaaaaacc tttagttttg
    20401 acgaagcagc agcatttatt atcgaaaact ttaacaaatt cagtccaaaa atggctaaat
    20461 ttgcccaatc tgcttttgaa aagagctgga tcgaagcaga agatcgtcct gggaaacgtc
    20521 ctggtggtta ttgtactgag ttaccagaaa caaaagaatc aaggatcttt atgacctaca
    20581 gtaattctgt taatgaggtt gcgacacttg ctcatgaact aggacatgct ttccatagca
    20641 gcacgatgtg ggatttacct tctttaaatc gcgaatatgc catgaatgtc gcagaaacag
    20701 caagtacatt cgcagaactg atcgtagccg atgctacatt aaaagtcgca aaaacaaaag
    20761 aagaaaaaat caatttgcta gatacaaaac tgcaaaatgc tttagctatg ttcatgaata
    20821 tccattcccg attcattttt gaaaatcgat tctatgaagc acggcaagaa ggattagtct
    20881 cagaagataa gatcacagag atgatggttg aggcgcaaaa agaaggctat caaggagctt
    20941 tagctactta ccatccatat ttctgggctg caaaattgca tttctttatt gatgacgtac
    21001 cgttttataa cttcccatac acatttggct acctctttag tttgggcatc tatgcttacg
    21061 ccaaccaaaa gggagcaaat tttgaagaag aatatatcca tttactacgc gatactgcct
    21121 caatgacgac ggaagatctt gcgaagaaac atttgaatgt cgatcttacc caaccagatt
    21181 tttggcaagc aggtatcgac atggtgactg aagatatcca aacgttcatg gaattgacag
    21241 aagaatttat ctgatttcta ttttatcaca a
//
LOCUS       DAZKLD010000040        20905 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.040, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000040 DAZKLD010000000
VERSION     DAZKLD010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20905)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20905)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20905
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.040"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            101..3379
                     /locus_tag="ACG8KR_001191"
     CDS             101..3379
                     /locus_tag="ACG8KR_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HFQ0200574.1"
                     /translation="MLKKRIKYSALMMNLLMLSTPVLISVVEVAANEQQSVNEENAHV
                     NIEGMSTMEEIPRTSEDMDTEEGNDPMMKESENEKTKKQSEEANEEWKGVVFGESTSA
                     SRYAIEPLEDGVRLSSTNNGGKFQSSGSDGMTYYYQAIPKDINFRMRATIEIESWTYT
                     NAQEGFALMVRDAVPKNHLFGQAFYSNSFAILGSRIEYVWDSDRQEVVNTSGSKYTMR
                     LGLGTRAITGISSEDPQAAPAPGSVSVETTPLETSAGFLEKETGSYNIFGNGHGNLFP
                     ATNSITKLDVEMKKTNTGLEAYYYDPETGEEMASDIMYDWEKLYASDESVIYAGFAAA
                     RNMTIKVEGIEVAYSDPATDPPGQERPTRLIDPIYTVTSSNHSGNQDHTMSFRANADG
                     LLTIKNKATGEVLKNAKNLAITANREFDYQTKLMEGTNEFTFSFTPDKNYPPEEYVEM
                     TSYETKEILHTVDYRKPKYSTVYVTPEGSDAGNGSRQNPLSLTQALRYAYAGQTIVMA
                     PGTYSFERGLTIPKGVNGTRENPIQLIAEKNPENKRPVLDFKGTGNGMTLFSHYWGLR
                     GFDITNSAAMQKGLQISGNHNLIEEIHAYRNGNTGIQISGSSNDPFEAWPAHNLVKNC
                     TSFENADPGFEDADGFAAKLTIGEGNVFDGCIAYHNADDGWDLFAKNETGSIGKVIIK
                     NSVAYRNGWVPGIEGEGNGNGFKMGGSSLTGPHELINSLSFENLAKGIDSNSGTDIIV
                     NSSTSFNNGSYNVALYTSSAGNTDYHASGILSFRTENLEMREQIRPLNQNEDQIYHSL
                     NYYWNEREKQSENTLGHTISKDWIESLNTCSKEGQQPVTRYENGSIHVHGLMQIKPGN
                     RQTEKERVDGLPLSVGAIFDGETSPSSEYPEYAIVQEPNQTKPNEQNNPIIIQEANGV
                     AENAEGNIIELKPFTVFVSTFTPNQGTPVFEDMRYFPEKIQIVETQSSAQFSNHTAIF
                     SAPSKGEYTLEVTYRSEIFDGEKWSAHTESVTQQKAITVRETADVPPGNDKPERENPG
                     NESPNGKPNGKPSHPDRTAKESEKNGTQGLPKTGEFNNPLLALAGGILLIGVVVYVMK
                     QRKK"
     gene            complement(3920..5827)
                     /locus_tag="ACG8KR_001192"
     CDS             complement(3920..5827)
                     /locus_tag="ACG8KR_001192"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M13-type metalloendopeptidase"
                     /protein_id="HFQ0200575.1"
                     /translation="MNKERLKQDFYEAVNEEWQKTAKIPADKPATGGFQDLVDGIDQL
                     LMGETDDLLAHPEKVSSEKMKHFLAYYQLANDYDRRNELHAQPLQPIIEKVESLQSFD
                     ELNKQFPEWLLDTLPLPFNLDVDADMKNAQTNAFFAYPPSLFLPDKTYYASDHPNGAQ
                     LIGVFFDMAVQLLELAGKEKSQAEAITEQAVQFDKLIAPHVKSAEENADYSKMYNPQS
                     FDTFIKYTDKLDLKKLVTGLIGTTPDKVIVTDPVYFDHLNDILTEAHFPLFKSWMIIK
                     TVRSLSGYLSEEFRQVSGIYSRVLSGTDEAMPQKKAAYYLASGQFDQVVGDYYGKKYF
                     GEAAKKDVEQMVKKMIAVYKKRLEENDWLGDATKEKAVIKLNNLGIQVGYPDKIPALF
                     DQFKTVPADQGGTLLSNTLVFSKLTLKDRFSKWNKPVDRSEWEMSADTVNAYYHPFRN
                     VIVFPAAILQAPFYSLEQSSSANYGGIGAVIAHEISHAFDNNGALFDEYGNLNNWWTE
                     EDLTQFQNKAQAMIEEFDGLPFADGKVNGKLTVSENIADAGGLSCALEAAKTESDLSI
                     EEFFVNWATIWRTKAKKEYQQLLLQIDVHAPAKLRANVQPKNLDEFYETFGITEQDAM
                     YLAPEKRVRIW"
     gene            5957..6832
                     /locus_tag="ACG8KR_001193"
     CDS             5957..6832
                     /locus_tag="ACG8KR_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide dehydrogenase"
                     /protein_id="HFQ0200576.1"
                     /translation="MKKRRRSQLNQVVDKPLYFKVDKKIKKLASTQQLQSRKSKLLFL
                     ALVFEDQSYVIIDQSGHPVEYSPAKYTYQEGLSCKKWKLINEEPIELSRWINRKEDIP
                     VLIEEKRSGKELANCWVGLPEERFLRYKKWATPSGYLCGTYAAAVLLAYYQDYRKGWM
                     LPNEIRKKNTADSLVLTKALRSQIQPLGLPTIPFQVSTGISSFLKKNGNHERARATLL
                     GSWQRATKRIREGKPVMIGILKVLGSTYGNHWVTAYAYFETETGERYYKVHDNWGDYH
                     KVIPASWSNGTVSLP"
     gene            6912..7886
                     /locus_tag="ACG8KR_001194"
     CDS             6912..7886
                     /locus_tag="ACG8KR_001194"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HFQ0200577.1"
                     /translation="MSNKYQDDTLKIFSLNGNRPLAEKIAKVFGTELGKSVVKQFSDG
                     EISINIEESIRGDHVYIVQSTNAPVNDYYMELLIMIDAMKRASAKTINVVLPYYGYAR
                     QDRTAKPHEPITAKLIANLIEEAGATRVLTLDLHTVQVQGFFDIPVDNLFTMPLFAHY
                     YRQLGLTGDDIVVVSPKNSGVQRARSLSEYLNSTLAIVDHADDASTEGGYVIGDVQGK
                     TCIMVDDILNTGATLARAANVLKENGAKEVYACASHGLLSDHAKSILDNAPIKDICIT
                     DSVFTEENRHPENLTIVTCSELMGEAVKRIHENTPMSPLFRLEEKEFE"
     gene            8066..9211
                     /locus_tag="ACG8KR_001195"
     CDS             8066..9211
                     /locus_tag="ACG8KR_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HFQ0200578.1"
                     /translation="MNEVKTIYLDHAATTPMHPQVIEAMTAIMQDTFGNPSSIHGFGR
                     HAHEKLETARQIIADSLQAKPHEIIFNSGGTEGDNTAILETAFSRQKEGRHLITTAIE
                     HPAVLNTMKYLETKGFDVTYLPVDEKGNLSIEEVKKALREDTILVSVMYGNNEIGNLM
                     PIKEIGELLADHPAYFHTDAVQAYGNQTIHPEELGIDLLSISAHKINGPKGVGFLYKR
                     DGVNIPALLLGGEQEEKRRAGTENLAGIWGMAKAVELLSPEEREARNNKYKGFQQLIL
                     DTLEANEIPYQVNGDLTNKLPHVLNLRLIGEANDLLLMKLDLNGVAISTGSACTAGNI
                     EPSHVLEAMYGEDTPILKESIRISFGLGNTEDDIQYFLDQLVRAVKK"
     gene            9348..9692
                     /locus_tag="ACG8KR_001196"
     CDS             9348..9692
                     /locus_tag="ACG8KR_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HFQ0200579.1"
                     /translation="MAFETTATVLGAPISYRLHPDAKRYTLRDNGFTETNGGNYQLIR
                     PLDATPQSKEGFKLKITVSKDIQTLKMSITTANGLKAVNIFKDPKQKMSQDKFYFLMD
                     GMISRGLFEKVE"
     gene            9963..11249
                     /gene="efmA"
                     /locus_tag="ACG8KR_001197"
     CDS             9963..11249
                     /gene="efmA"
                     /locus_tag="ACG8KR_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EfmA"
                     /protein_id="HFQ0200580.1"
                     /translation="MENEQSVVLTNWKRNYLFFLSGQFLSGITSMVVQYAIIWYLTRE
                     TGSATILSFATLLGMIPMVLLSPFVGPLVDRWDKKALLIVTDIIVAIFALILAVVGTI
                     SESFPIWLVFVSLFMRSVAQTFQMPTIQSIMPTIVPSSHITRTNGQLGMVQSANFIIA
                     PALGAALFSVVPVNYLILLDVLGAVFGVGLLIFVKIPKVSPEILEVPLTIFKDAKFGL
                     QQLMDNKGLWYITINGAFVMLLFMPAISLYPLMTLDYFGGSVGQAGAVEVVYAVGMLL
                     GGALISFIGTWKDRMKPIIIAYIIMGLTIGASGLVPNDSQGFLYFLILNAGAGCATPY
                     FNTLLMAMIQQSYESNVLGRVLGNFNSLMNLAGPIGLLFAGPLADRLGVEKMFLFSGI
                     GILLCGIVLFLTSAARNYDKELQKKLVKEHHEQKDE"
     gene            complement(11387..11986)
                     /locus_tag="ACG8KR_001198"
     CDS             complement(11387..11986)
                     /locus_tag="ACG8KR_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFQ0200581.1"
                     /translation="MNYFISDMHFFHERLLGKNDFAPRLFANVDEMNQQLITSWNAVI
                     KETDHVYHLGDLAMHPQYEKANAEILELVTQLQGTIHLIKGNHDSRAFFRYLEKNDPR
                     LTDGSPKFYFYDVGAIVKFDHQQYYLTHYPMLLGPNDTIRNLHGHIHHYSVPIENDVN
                     VGVDAPERELLKEKLPFGTPLSEQQIKEIYEAKRKYDRS"
     gene            12390..13511
                     /gene="mnmA"
                     /locus_tag="ACG8KR_001199"
     CDS             12390..13511
                     /gene="mnmA"
                     /locus_tag="ACG8KR_001199"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HFQ0200582.1"
                     /translation="MTDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVAKVAAQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMDLGADYVATGHYAQVTRDENGVVHMLRGVDNNKDQTYFLSQLS
                     QEQLSKTMFPLGGMEKSEVRAIAERAGLATAKKKDSTGICFIGEKNFKQFLSNYLPAK
                     KGNMVTLDGEVKGQHAGLMYYTIGQRQGLGIGGGGDSQEPWFVVGKDLATNTLYVGQG
                     FHHPALYATSLDASEIHFTTNEPMPKEFKCTAKFRYRQQDVPVTVRLLDDNRAEVVFD
                     EPVRAITPGQAVVFYDGMECLGGGLIDHAYQETKVLQYV"
     gene            13757..15178
                     /gene="arcD"
                     /locus_tag="ACG8KR_001200"
     CDS             13757..15178
                     /gene="arcD"
                     /locus_tag="ACG8KR_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0043858 - arginine:ornithine antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HFQ0200583.1"
                     /translation="MEKKSTGISKWGLVALVVSSSIGSGVFGITSDLASSAAPGPAIL
                     SWIIVGIGILALVLSLNHLGEKRPDLDGGIFGYAEASFGKLGGFISGWGYWLSAWLGN
                     VAFATMLMSAMGEFFPIFKGGQNVPSILFASIFIWGLTLLVNNGVEGASVINTIVTIC
                     KLVPLFLFLIFVFIAFKVNIFTADFWGNVSDNLVNGTGQQGTIWLQIKGCLMVMMWVF
                     VGIEGASVLANRAKKRSEAQQASIIGLLCLLFIYILASILPYGVLSQEELATISQPAM
                     ANILKGIVGNWGAALINIGLIISIIGSWLSWTMLPAETTMLMARDGLLPKGWGKTNKK
                     NAPTYSLVVTAILTNLFLLTFLVTDYAYQFAYSLCTAAILICYLLVGIYQVQFSLKEG
                     IKKQVVIGGIAVVFELMGIILAGFSYVLLCSIAYLPGFCFYWIACRETKHQITGKEKV
                     MIGLICVAALLSIGLLAVGWIKI"
     gene            15192..16310
                     /locus_tag="ACG8KR_001201"
     CDS             15192..16310
                     /locus_tag="ACG8KR_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HFQ0200584.1"
                     /translation="MKIANFGVEEWLNKWEKKAVYDIAQSSIEALTLDELVGLDGTSV
                     SKFFEKRKNEPLDYGWIEGSDVFKQEVASLYQTVDPENILQTNGATGANLLALYALVE
                     AGDHVVSMLPTYQQLYDIPKSLGAAVDFVHLKEEEDWQFDLEQLEALVKPETKIICLN
                     SANNPTGTLLDRKTLEKIAEIARTVDAYVLIDEVYAPLTDKGEFLSIVDVYEKGIATN
                     SLSKTYSIPGIRIGWTATGPELAEVFRKYRDYTMICGGVLSDDLAVHALKNKEKILAR
                     NKKIITENLVILKQWVANEPKVELVAPNYVSTSFIKLAIEEDDQTFCINLLEETGVLL
                     VPGSAFDLPKHARLGYCCKKETLEKGLFLLSEFLKKQA"
     gene            complement(16370..17662)
                     /locus_tag="ACG8KR_001202"
     CDS             complement(16370..17662)
                     /locus_tag="ACG8KR_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HFQ0200585.1"
                     /translation="MAARIKEKLFRKRETWEQNLFVLWFGTFMAGIGFSLVMPFMSLY
                     IDTLGNYSTSQLNFWSGLTFSSTFLVTTLISPWWGKLADQKGRKLMLLRASLGMAIVI
                     SLMGCVTSVYQLVALRLLQGIFSGYISNATALVATGTPKEKSGQVLGTLATGSVTGTL
                     LGPLLGGISASAFGYRPTFFITGIILFFVFLLSLFFVHEKFVPVKKENMVSAKQIFKD
                     LKYPHVVIGMFVTTMIIQASNNSISPIISLYIRQLLHGHGNVTLISGIIASIPGIATL
                     IAAPRFGRLGDKIGSERILAIGLGFAILVYIPMAFVTNVWQLAALRFLIGISDACLLP
                     AVQALITKYSPHQAAGRIFSYNQSFQATGNVVGPMIGSSVSSAFGYRGVFLSTSLLVL
                     VNFLLVRHNTQEIHQQETAEEKASGVSIASGHSPSHRQ"
     gene            17812..18372
                     /locus_tag="ACG8KR_001203"
     CDS             17812..18372
                     /locus_tag="ACG8KR_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFQ0200586.1"
                     /translation="MKKRNLSQEKIIDCFRELAEEMDVQQITFQHLAKRLDIKAPSLY
                     NHFKNIRDVKTALTAKLLQELNDQLRRALVGKSGQEALQIYAETYRSFAFSNQAVYEL
                     LISVPHTNEAVLLDGIHETNQIILQIFEAFQLTKEEKVHRSRELRSMIHGYLSLRFLG
                     YFTKEPTIDPEDSYFWMINNFIATLP"
     gene            18501..18878
                     /locus_tag="ACG8KR_001204"
     CDS             18501..18878
                     /locus_tag="ACG8KR_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HFQ0200587.1"
                     /translation="MYQVITMFGDNEPWWFFEDWEEDIEEEETFESFEEARQAYEKQW
                     NEIHQNYEYINAKSNFLSAFWNDGDERWCEECDDDLQQYKGLALLKNHQPITVESRKE
                     FYETTNSSGKTKRCERPKQGAWC"
     gene            18811..20883
                     /locus_tag="ACG8KR_001205"
     CDS             18811..20883
                     /locus_tag="ACG8KR_001205"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_function: GO:0098847 - sequence-specific single
                     stranded DNA binding [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0051304 - chromosome separation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFQ0200588.1"
                     /translation="MKQLILAEKPSVAKDLSKVLGANQKHKNYYEGPKVIVTWALGHL
                     LGLKMPEDLNKEWQSWQMDTLPMIPKKLGIKPLPKTGHQLKAIKQLAQRKDVSEAVIA
                     TDAGREGELVARWILEYVHFNKPVKRLWISSQTDKAIKTGFKQLRPAKEYDALYESAL
                     ARAKADWLVGLNVTRALTVKYADNLSAGRVQTPTLALVRDQEKKIEQFRPQTYFTITL
                     QAGKEQAKMLQKNQFALKTQEEAQKLVQLISTSKGTVTEVQEKLKTESAPLPYDLTEI
                     QREANQRYGYSAKKTLSLVQSLYETHKIVTYPRTDSKYLTNDMKSTMKERLQAVSDFA
                     PEVKQYLKNGAIVQQTKVFQDSKVTDHHALLPTENRARYEKLSNEERKIYQMIVSRFL
                     GLFAQPHKVSQTKVTVEFGKERFIFRQNRVVQAGWKGETETETETVKWEKGMRINPDF
                     AIKKELSAPPKPLSEASLLGQMEKYSLGTPATRAEIIEKLIKSELMERTPQGLQVSPK
                     GKQLLVLVNPSLVTPDLTESWEKDLEAIAAGKKQAGTFLNGIEKETKRLVNEIKSSKQ
                     EYQDFSITQKKCPECGANLREKNTRDGKIYVCTNSDCNYRRRKDPKVSNHRCPQCHRK
                     MEIIEGKNGAYFRCKYDGTTEKLLDKKEQKKKMTKHEERRLLKKYAQAEEPEESPLAA
                     ALKAAMKK"
BASE COUNT         6774 a         3792 c         4467 g         5872 t
ORIGIN      
        1 taaaatacac ttattataat tttgtttttt attgacagta aaaataaatt aggtaatact
       61 gtaagtaaac gctttctaat ttgttacggg aggaatacac ttgttaaaaa aacgtataaa
      121 atacagtgcg ctaatgatga atctccttat gctcagtacc ccggttttaa tttctgtcgt
      181 tgaagttgct gctaacgaac aacagtctgt gaacgaagaa aatgcacacg tgaacattga
      241 agggatgtca acaatggagg aaataccaag aactagtgaa gatatggata cggaagaagg
      301 aaatgatcct atgatgaaag aatcagaaaa tgaaaagaca aaaaagcaat ctgaagaagc
      361 aaatgaagag tggaaagggg ttgttttcgg agaaagcaca agtgctagta ggtatgccat
      421 tgaaccatta gaagacggcg tgcggctctc ttccacgaat aacggcggaa aattccagtc
      481 aagcggatca gatggaatga cctattatta tcaagcaatc cccaaagaca tcaactttag
      541 gatgcgggcg acgatcgaaa tagaatcttg gacctatacg aatgcccaag aaggatttgc
      601 tttaatggta agagacgcag taccaaaaaa tcatttgttt gggcaggcat tttattcaaa
      661 ttcatttgcc atactaggca gtcggatcga atatgtctgg gactcggata gacaagaagt
      721 ggtcaacacg agcggcagca aatatacgat gcgacttgga ttggggacac gggcaatcac
      781 aggaatatca tctgaagatc cgcaagctgc gccagctcct gggagtgtct cagtggaaac
      841 aacaccttta gagacttcag cagggttctt ggaaaaagaa acaggttctt ataatatatt
      901 cggaaacggt catgggaatc tgtttccggc cacgaactcg attactaaac tggacgtaga
      961 gatgaaaaaa acgaatactg gtttagaggc atattattac gatcctgaaa caggagaaga
     1021 aatggcttct gacatcatgt atgactggga aaaattatac gcttcagatg aatcggttat
     1081 ttatgcagga tttgctgcag caaggaatat gacgatcaag gtagaaggaa tcgaagttgc
     1141 ttactcagat cctgcgacag atcctcctgg gcaagaaaga cctactcgct tgattgatcc
     1201 gatttacact gtgacctcta gtaatcattc aggaaaccaa gatcatacta tgtcctttag
     1261 agcaaatgct gacggattat tgacaattaa aaataaagca acaggagagg tgcttaagaa
     1321 cgcaaaaaac ttggctatca ctgcgaatcg ggaatttgat tatcagacaa aactgatgga
     1381 aggaacgaac gaatttactt tcagttttac gccagataaa aactatccgc cagaagaata
     1441 cgtagagatg actagttatg aaacaaaaga gattcttcat acagtggact accgtaaacc
     1501 taaatattcg acagtttatg taacaccaga agggtcagat gcaggaaacg gaagcagaca
     1561 aaatccatta tctctgacac aagcattgag atacgcatat gctgggcaga caatcgtcat
     1621 ggcgcctgga acttattcct tcgaaagagg attgacgatt ccaaaaggcg ttaacgggac
     1681 aagagaaaac ccgattcagt tgattgcaga gaaaaatcca gaaaacaagc gtcctgtact
     1741 tgattttaaa ggaactggaa atgggatgac actgttcagt cactactggg gtttacgagg
     1801 gtttgacatt acaaatagtg cagctatgca aaaaggatta cagatttctg gaaatcataa
     1861 tttgatcgaa gagattcatg cttatcggaa tggaaataca ggaatccaaa tcagcggtag
     1921 ctcaaatgat cctttcgaag catggcccgc gcataactta gtcaagaact gtacatcttt
     1981 tgaaaatgcc gatccaggat ttgaagatgc cgatggattt gcggccaaac tgacaatagg
     2041 agaaggaaat gtatttgacg gttgtatcgc gtatcataat gcagatgacg gttgggacct
     2101 ttttgctaaa aatgaaacag gttcgatcgg aaaagtaatc atcaaaaact cagttgctta
     2161 tcgaaacggc tgggtaccag gaattgaggg agaaggtaac ggtaatggat tcaaaatggg
     2221 cggttcttct ttaacgggcc ctcatgaatt gatcaatagt ttgtctttcg aaaatcttgc
     2281 gaaaggaatc gattccaata gcggaacaga tattatcgtc aatagcagta caagttttaa
     2341 taacggttca tataatgtgg ctctttatac ctcttcagca ggaaacacag actaccatgc
     2401 ttctggtatc ttatctttcc gaacagagaa tcttgagatg agagagcaga tccgcccatt
     2461 gaatcaaaac gaagatcaaa tctaccactc gttgaactac tattggaatg aacgagagaa
     2521 acaatcagaa aatactcttg gacataccat atcaaaagat tggatcgagt ctctaaatac
     2581 atgttcgaaa gaaggtcagc agcctgttac gcgctatgaa aatggaagta tccatgttca
     2641 tggactgatg cagattaaac cggggaatcg tcaaacagaa aaggaaagag tggatggtct
     2701 tccattatct gtaggtgcta tttttgacgg agaaacctca ccgtcttcgg aatatccaga
     2761 gtatgcaatc gtacaagaac caaatcagac aaaaccaaat gaacaaaata atccgattat
     2821 tatacaagaa gcaaatggtg tggcggagaa tgcagaagga aatatcattg aactgaagcc
     2881 ttttacagtt tttgtgtcaa ctttcacacc aaatcaagga acgcctgtct ttgaagatat
     2941 gcgttatttc ccagaaaaga tccaaatcgt tgaaacacag tcaagtgctc aattttctaa
     3001 tcataccgct attttttcag caccttcaaa aggagaatat acgctagaag tcacttatcg
     3061 ttcggaaata tttgatgggg aaaaatggtc tgctcataca gaatctgtta cccagcaaaa
     3121 agcgatcact gtgcgagaaa ctgccgatgt gcccccggga aatgacaaac cagaaagaga
     3181 aaatccagga aatgaatcac caaatggaaa accaaatgga aaaccttctc atccggatag
     3241 gacagcaaaa gaaagtgaaa aaaatggcac tcaaggactc ccaaaaacgg gagaatttaa
     3301 taacccgttg ctagcattag ctggaggaat tttgttaatc ggtgtagttg tttatgtcat
     3361 gaaacagcga aaaaaataag tggaatttct ccttttatga tgtaaaacaa ttgactgagg
     3421 aaagtaaaaa tagaaggaaa tgagaccaat agatgtctca cttccttcta tctttttata
     3481 gatgaggttc tccttgctgc ttttgattgg ctgttcttat tacttgatgc aggacagctt
     3541 tttcacaatc tctccactaa acggtgttca atcagctgac cctcggtatt agctcaacaa
     3601 acggcaaaat atgaggaact atttttccgt ttttttctct aatactcagg tcaggacgct
     3661 gatttcacaa cctctcgata aacggtgttc aaaagccagt ttctcggtag taagtcaaat
     3721 ttatgcaaaa atgtggaaag ccattttcac aaaatttttc ttactacttg aaactaagca
     3781 gctttttcac aacctctaat acatggtatt ccagaagtaa cttcttcgaa aaataagccg
     3841 aaatgtcaca aaaatttgag aagcgatttt cccaaattcc ttcttatttc ttgaagctga
     3901 ccacttctgg cacaacctct taccagattc gcactctttt ttctggcgct agatacatag
     3961 cgtcttgttc cgtgatacca aatgtttcat agaattcgtc aaggtttttt ggttgcacgt
     4021 ttgctcggag tttagcaggg gcatggacat cgatttgcag taaaagttgt tggtattctt
     4081 tttttgcttt tgtacgccaa atcgttgccc aattgacaaa gaattcctcg atagaaagat
     4141 ctgattccgt ttttgctgct tccaatgcac agcttagacc gcctgcatca gcaatatttt
     4201 ccgaaacggt taattttccg tttacttttc cgtcagcaaa aggcagacca tcgaattctt
     4261 cgatcattgc ttgtgctttg ttttgaaatt gtgtaagatc ttcttctgtc caccagttgt
     4321 tcaagttgcc atattcgtca aataaagcgc cgttattatc aaatgcatgg gaaatctcat
     4381 gagcgataac agctccgatt ccaccataat tagcagagct tgattgttct agactgtaaa
     4441 atggtgcttg aaggatcgct gccggaaaga cgatcacatt ccgaaatgga tgataataag
     4501 cattgaccgt gtcagcactc atttcccatt ctgaacgatc cactggtttg ttccatttgc
     4561 tgaaacgatc ttttaatgtg agtttgctga aaacaagagt atttgaaagc aaagtaccac
     4621 cctgatctgc aggtactgtt ttgaactggt cgaataatgc tgggatctta tcggggtagc
     4681 ctacttggat acctaagtta tttaatttta tgacggcttt ttcttttgtt gcatccccaa
     4741 gccagtcatt ttcttctaga cgttttttat aaacggcgat catttttttc accatttgtt
     4801 ctacgtcttt tttagcagct tcgccaaaat atttttttcc gtagtaatcg ccaactactt
     4861 gatcgaattg tccagaggca aggtaatacg ctgctttttt ctgaggcatt gcttcgtctg
     4921 ttccagacaa tacgcgagaa tagattccgc tgacttggcg gaattcttct gatagataac
     4981 cactaagaga acgtactgtt ttaatgatca tccagctttt gaataacgga aaatgagctt
     5041 ctgtcaggat gtcattcaaa tgatcgaagt acactggatc agttacgatc actttatctg
     5101 gagttgtccc aatcaaaccc gtgacaagtt ttttcaagtc cagtttatct gtgtatttaa
     5161 taaatgtatc aaaagattgc ggattataca ttttgctgta atctgcgttt tcttctgcac
     5221 tttttacatg aggagcaatc aatttatcaa attgcactgc ttgttccgtg attgcttctg
     5281 cctgactttt ttctttgccg gcaagctcta ataactgaac agccatatca aagaaaacgc
     5341 ctattagttg tgcaccgttg ggatggtctg atgcataata cgtcttatct ggaaggaata
     5401 aagaaggcgg ataagcaaag aaggcgttcg tttgcgcatt cttcatatct gcatctacat
     5461 ccagattaaa tggcaatggt aatgtatcca acagccattc tggaaattgc ttattcaatt
     5521 cgtcgaaaga ctgcaatgat tcaacttttt cgattatagg ttgaagcggt tgagcatgca
     5581 actcatttct tcggtcataa tcgtttgcca gttgataata tgcaaggaaa tgtttcattt
     5641 tttctgaaga cactttttct ggatgggcaa gcagatcgtc tgtttctccc atcaaaagtt
     5701 ggtcgatccc gtcaactaaa tcttggaaac cgcctgtagc tggtttatct gcagggattt
     5761 ttgctgtttt ttgccattct tcattgactg cttcatagaa atcttgtttc aatcgttctt
     5821 tgttcattca tcgatcgctc cttttttgtt tcgtgtgccc attatcttac aaaatgaagg
     5881 aattaacaaa tagaacataa aaaattgaaa attcgaacaa tcaaactgat ttgttttttt
     5941 caatccttca cttataatga aaaaaaggag gcgatcccaa ctgaatcaag tagtggataa
     6001 acctttatat ttcaaagtag ataaaaaaat taaaaaatta gccagcactc agcagctgca
     6061 gtcaagaaaa agtaaattgc tttttttagc tctggttttt gaagatcaaa gttatgtgat
     6121 tatcgatcaa tcaggacatc cggtagaata ttctccagca aaatatactt atcaagaggg
     6181 tctttcttgt aaaaaatgga agctgatcaa tgaagagcca atcgagttat ctagatggat
     6241 caacagaaaa gaagatattc ccgttttgat cgaagaaaaa aggtcaggaa aggaactcgc
     6301 aaattgctgg gtgggattgc cagaagaacg ttttttgagg tataagaaat gggctacccc
     6361 ctcaggctat ctttgtggta cttacgcggc agcagttctc cttgcctatt atcaagacta
     6421 tcgtaagggg tggatgcttc caaatgagat tcgcaagaaa aatacggctg attcgttggt
     6481 tttgacgaag gcattgcgta gccagatcca accactagga cttcctacga ttccttttca
     6541 ggttagtaca gggatttcta gttttttaaa gaagaatggc aatcatgagc gtgcacgagc
     6601 aactcttcta ggttcatggc agcgagctac aaaacgtata cgtgaaggca aacctgtgat
     6661 gattggtatt ttgaaagtac ttggcagtac atatggcaat cattgggtga cagcgtacgc
     6721 ttattttgaa actgaaacag gagaacgtta ttacaaagta cacgacaact ggggcgatta
     6781 tcataaagtc attcctgcca gttggagtaa cggaacagtt tcacttcctt aagaatttgc
     6841 tgaaattaac agaaaatctt ggaaaaatat gttaaactat cgaagaatag agaagttttg
     6901 gggaggacta catgagtaat aagtatcagg atgacacgtt gaagattttt agtttgaatg
     6961 gcaaccgtcc attagctgaa aaaatcgcga aagtgttcgg aacagaatta ggtaaaagcg
     7021 tcgtgaagca atttagtgat ggcgagattt ccatcaatat cgaagaaagt attcgagggg
     7081 atcatgtgta tatcgttcaa tcaacgaatg cacctgtcaa tgattactat atggaactac
     7141 tgatcatgat cgatgcaatg aagcgagcaa gtgcgaaaac gatcaatgtc gtattgcctt
     7201 actatgggta tgctaggcaa gaccgcacgg cgaaacctca tgaaccgatc actgcgaaat
     7261 tgatcgctaa cttgatcgaa gaagcagggg caactcgagt actgacttta gatctgcaca
     7321 cggttcaagt acaaggattc tttgatattc ctgtagataa tttatttacg atgccgttgt
     7381 ttgctcatta ttaccgtcaa ttaggtctga cgggagatga catcgtagtt gtttcgccaa
     7441 aaaacagcgg tgttcagcgt gcacgtagtc tttcagaata tttgaacagc acattagcca
     7501 tcgtagatca tgcagatgat gcttcaactg agggtggtta tgtgatcggt gatgttcaag
     7561 gaaagacatg tatcatggta gatgatatct tgaacacagg agcaacgctt gcacgagcag
     7621 caaatgtatt aaaagaaaac ggcgcaaaag aagtatatgc ctgtgcgtca catggattat
     7681 tgtccgatca tgcgaaaagt attttagaca atgcaccaat caaagatatt tgtatcactg
     7741 attctgtttt cacagaagaa aatcgtcatc cagaaaactt aacaatcgtt acatgttcag
     7801 aattaatggg tgaagcggtc aaacgcatcc atgaaaatac accgatgagt ccgttattcc
     7861 gtctggaaga aaaagaattc gaataaacaa aaaaaagagg gaaagaagtt gtgacagaag
     7921 tgagcagctt caaggagctg cgacaagact tttgtcacag ctcctttttt ctatgcccat
     7981 gaaacgtcct ctagacaaaa atgagctctt ctttggtttg ttcgctattt tatgctagaa
     8041 tgtgcctagc aataatttgg aagggatgaa tgaagtgaaa actatttatc tggatcatgc
     8101 ggcaactacc cccatgcatc cacaagtaat tgaagcgatg acggcaatca tgcaagacac
     8161 gtttggaaat ccttcaagca tacatggatt tggtcgccac gcgcatgaaa aattggaaac
     8221 agcaagacaa atcattgcag acagtctgca agcaaaacct cacgagatca tctttaacag
     8281 tgggggaaca gaaggggata atacagctat cttagaaaca gcgttctcgc gtcaaaaaga
     8341 aggcagacat ctaatcacta cagctatcga acatcctgct gttcttaata ccatgaaata
     8401 tttagagaca aaggggtttg acgttacata tctgcctgta gatgaaaaag ggaatctgtc
     8461 gatcgaagag gtcaaaaaag cgttacgaga agatacgatc ttggtctctg tgatgtacgg
     8521 gaacaatgaa atcggaaatt tgatgccaat caaagaaatc ggtgaacttt tagcggatca
     8581 tccagcttat ttccatacag atgcggtaca agcgtacgga aaccaaacga tccatccaga
     8641 agaattagga atcgatttat taagtatctc tgctcataaa atcaatgggc caaaaggcgt
     8701 cggtttctta tataaacgag acggagtaaa tatccctgca ttgcttttag gcggggagca
     8761 ggaagaaaag cggcgtgcag gtacagaaaa tttagcaggg atctggggta tggcaaaagc
     8821 agttgaactg ctgtcacctg aagaacgtga agcacgtaac aacaaatata aaggattcca
     8881 gcaattgatt cttgatacat tagaagcaaa cgaaatccct tatcaagtaa atggtgattt
     8941 aacgaataaa ctccctcatg tgttgaatct tcgcttaatt ggcgaagcaa atgatttgct
     9001 actgatgaag cttgatctta atggggtcgc tatttctact ggttcagctt gtacagcagg
     9061 aaatatcgag ccttcacacg tattagaagc aatgtatgga gaagatacac cgatactaaa
     9121 agaatccatc cgtatcagct ttggtttggg aaataccgaa gacgacatcc agtatttcct
     9181 tgatcaatta gttcgtgctg tcaaaaaata actagtgaag tcattcttat atgaacagat
     9241 gtctgagaga acgccttcat tttttaggga acttaactga aaaacctagt aaaaatcagg
     9301 ataaactttt ataataaaga gaaactaatt ttatcgaggt gaaaatcatg gcatttgaaa
     9361 caacagcaac ggtactgggc gcaccaatta gctatcgttt gcatcctgat gcaaaaagat
     9421 atacattgcg cgataatgga tttacagaaa caaacggagg caattatcaa ttgatccgac
     9481 cattagatgc cacgccgcaa agcaaagaag gattcaaatt aaaaatcact gtatcaaaag
     9541 atattcaaac attgaaaatg tcgatcacta cagcaaatgg attgaaagca gtcaatattt
     9601 tcaaagatcc aaaacagaaa atgagccaag ataaatttta ttttctgatg gatgggatga
     9661 ttagccgtgg gttattcgaa aaagtcgaat gatctgtgga tataaaaaga caactagttt
     9721 tgaaacggcc tattgttaga tgactaactt tgtaaggacg acgcaaatta gttgtctttt
     9781 ttatttttaa atttaacaca tgttgttatt tttaatattt tgctgattta ttgtttctaa
     9841 gtaaagaaat ttaaattgaa acgattgtta tttagggaaa aaggtatttt ttgaaaaaag
     9901 aatcccgtat aatgattgtt gtgcaaacgc tatacaaaat gaaaaagaaa agagggatgt
     9961 ttatggaaaa tgaacagtcg gtcgttttaa cgaattggaa gagaaattat ttgttctttt
    10021 tatcgggaca gtttttgtca ggaatcacta gtatggtcgt ccaatatgcg atcatctggt
    10081 atttgacaag agaaaccggt tcggcgacaa ttttgagttt tgctacttta ctaggaatga
    10141 ttcccatggt tttactaagc ccatttgtag gaccgctagt ggatcgctgg gataaaaaag
    10201 ctcttttgat tgtcacggat attattgtag cgattttcgc gcttatttta gcagtagtcg
    10261 gaacgatttc agaatccttc ccgatttggc tagtttttgt gtctttgttt atgcgttcgg
    10321 tcgcacaaac attccaaatg ccaacgatcc agtcgattat gccgacaatc gttccatctt
    10381 ctcacataac aaggacgaat ggacagttag gaatggtcca atcagcgaat ttcatcattg
    10441 cgccagctct aggtgcggct ttgttttcag tcgttcctgt caattacctt attttattag
    10501 atgtattggg ggcagttttt ggtgtcggct tattgatttt tgtgaagatc ccaaaagttt
    10561 ctcctgaaat attggaagtt ccgcttacta tttttaaaga tgcaaaattt gggctgcagc
    10621 aattgatgga taataaaggg ttatggtaca taacgatcaa tggtgcattt gtgatgctgt
    10681 tgttcatgcc agcaatcagt ctgtatccat tgatgacgct agattacttt ggtggttccg
    10741 ttggacaggc gggagcagtg gaagtagtct acgcagtcgg aatgctctta ggcggtgcac
    10801 tgatcagttt tataggaaca tggaaagacc gaatgaaacc tattattata gcctacatca
    10861 ttatgggcct gacgatcggt gcaagcggat tggttccaaa cgatagtcaa gggttcttgt
    10921 actttcttat cttgaatgca ggagcaggtt gtgcaacgcc atatttcaat acactgctga
    10981 tggcaatgat ccagcagagt tatgaatcga atgttttagg tcgtgtctta gggaacttca
    11041 actctttgat gaacctggca ggtccaattg gattattatt tgcaggacca ttagctgatc
    11101 gtttaggggt agaaaaaatg ttcttgtttt ccggaatcgg gattttatta tgcggaatcg
    11161 ttttattttt aacgtcagct gcccgaaatt atgataaaga attacagaaa aaactggtaa
    11221 aagaacacca tgagcaaaaa gacgaatagt atggaatgga gttgtgacag aagtcttgtt
    11281 acaactccat ttctgatcct aatgttcctg tggcaataag cctactcaaa tgaaaaatat
    11341 gagtaattat ctttatttgt ttaatacttg atatagggca gttttttcac gacctatcgt
    11401 acttcctctt tgcttcatag atttctttga tttgttgctc agataacgga gtgccaaatg
    11461 gtagtttttc ctttaagagt tctctttccg gtgcgtctac gcctacattg acatcgttct
    11521 cgataggtac gctgtaatga tggatatgtc catgaagatt gcggatagtg tcgttaggcc
    11581 cgagcaacat cggataatga gtcaagtaat attgttgatg gtcgaattta acgatggcac
    11641 ctacatcata aaaataaaat ttgggacttc cgtcagtaag cctaggatca tttttttcta
    11701 aataacggaa aaaagcgcga ctgtcatggt tccctttgat caagtggatt gttccttgca
    11761 gttgagtgac cagttccaaa atttctgcat ttgctttttc atattgcgga tgcatagcaa
    11821 ggtcgcctag atgataaaca tggtcggttt cttttattac tgcattccag ctagtgatca
    11881 actgttggtt catttcatcc acatttgcaa agagccgcgg agcaaagtcg ttttttccta
    11941 ataagcgttc atggaaaaag tgcatatcac tgataaagta attcatgtat cttttgcctc
    12001 ccttttttct tagagtaaac aaaatcactt atctgagcaa ctcataacat ctaaaaagaa
    12061 aaggttgtaa atgagacgtg cagctttaag cgataagaag caattttgaa aaatagtttt
    12121 tcacaattta taaaattatt ccttattgcc gaaaggctgg ccctttttat gagtatgcca
    12181 attttttata tcgaatgctc aaaagatgag tgattttttg gttgactatc atgtttgtgt
    12241 actgaatagg tccagtagaa cagtattttt acgacttgat aattaaggga tgaaattttc
    12301 agaaatatga cgtataatgt aaaatactga aaagttgcga ccttcaactc gtagatgttt
    12361 cagaatctaa ttgaaatgat ggtgatatca tgacagacaa cagcaaaaca cgcgttgtcg
    12421 tagggatgag cggcggtgtc gactcatctg taacagcact tttgttaaag gaacaaggat
    12481 acgacgtgat cggaatattc atgaaaaatt gggatgacac ggacgaaaac ggtgtatgca
    12541 cggccacgga agattataag gatgtagcga aagtagctgc tcaaatcggg attccttatt
    12601 actctgtgaa ttttgaaaaa gaatattggg atcgtgtatt tgaatatttc ctagccgaat
    12661 accgcgcagg acgtacacct aacccagacg tgatgtgcaa caaagaaatc aaattcaaag
    12721 cattcttgga ttatgcaatg gatcttggcg ccgattatgt agctactgga cattatgcgc
    12781 aagtcactcg cgatgaaaac ggcgtggtcc acatgcttcg tggagtagac aataataaag
    12841 atcaaactta cttccttagc caattatcac aagaacagct ttcaaaaacg atgttccctc
    12901 taggcggcat ggaaaaatca gaagtacggg caattgctga acgcgcaggc cttgcgactg
    12961 ctaagaaaaa agattcaacg ggaatctgct ttattggtga aaagaacttc aaacaatttt
    13021 tgagcaacta tctgccagca aaaaaaggaa atatggtcac tcttgatggt gaagtcaaag
    13081 gacaacatgc tggcttgatg tattatacga ttggtcaacg tcaaggacta gggatcggtg
    13141 gcggaggaga ttcgcaagaa ccctggtttg ttgtaggaaa agatctagcg acaaacacat
    13201 tatatgtggg gcaagggttc catcatcctg cattgtatgc cacaagcttg gatgcaagtg
    13261 aaatccactt tacgacgaat gaaccaatgc caaaagaatt caagtgtaca gcgaaattcc
    13321 gttatcgtca gcaagacgtt cctgtgacag ttcgcttatt ggatgataat cgtgcagaag
    13381 tcgtattcga cgaacctgta cgtgcaatca ctcctggaca agcagttgta ttttatgatg
    13441 gaatggaatg tctgggtggt ggtttgatcg accacgcata tcaagaaaca aaagttttac
    13501 aatacgttta agcgatatta aatgaatcaa aacagctatg gctaacaaaa gaaacacctt
    13561 ttcttttgga tagcatagct gtttttttgc aataaaatta atttttattc attttatact
    13621 tgaaatactt gtgtaaaaag ttcataatat ccaataatat gaataaagtt gctttttgtt
    13681 aagcaagagc agttgcaaaa tagaagggaa tcttttgcaa gtgatttgtt caattttata
    13741 agtgagggtg atggaaatgg aaaagaaatc gacgggtatt agcaaatggg gactggttgc
    13801 tctggttgtc agttcgtcaa tcggaagcgg tgtgtttggg atcacaagtg atctggctag
    13861 ttctgcagct ccagggccag cgatcctttc atggataatc gttgggattg gtattttagc
    13921 tttggtatta tcgctgaatc atttaggaga aaagcgtcca gatctagatg gtggtatttt
    13981 cggttacgcg gaagcttcat ttggtaaatt aggtggcttc atcagtggtt ggggttactg
    14041 gttgtctgcg tggctgggaa atgttgcttt tgcaacgatg ctgatgagcg ccatgggaga
    14101 atttttcccg atattcaaag gtggacaaaa tgttccttct attttatttg caagtatttt
    14161 tatttggggt ctgactttat tagtaaacaa tggagttgaa ggagcgagcg tcatcaatac
    14221 aatcgtgacg atctgtaaat tagtgccatt attcctattt ttaatatttg tatttatcgc
    14281 atttaaagtg aatatattca ctgctgattt ttggggtaac gtatctgata atctagtgaa
    14341 tggaacaggg cagcaaggaa cgatctggtt gcagattaaa ggttgtttga tggttatgat
    14401 gtgggtcttt gtagggatcg aaggcgcaag tgttttagcc aaccgcgcga agaaacgttc
    14461 agaagcccag caagcatcga tcattggtct gctttgttta ttgtttatct atatccttgc
    14521 ttcgatcttg ccatacggcg tcttatctca agaagagtta gcaactattt cacaacctgc
    14581 tatggcaaat attttaaaag gaatcgtggg aaattgggga gcagcgctga tcaatatcgg
    14641 cttgattatt tcgatcattg gttcttggtt gtcttggacg atgctcccag cagaaacaac
    14701 gatgttgatg gctcgagacg gtctgttgcc gaaaggttgg ggaaaaacga ataaaaaaaa
    14761 tgccccaact tattcgttag ttgttactgc aatcttgact aatctattct tattgacttt
    14821 cttagttact gattatgcct atcaatttgc ctattcttta tgtacagcgg caattttgat
    14881 ttgttatcta cttgtcggga tctatcaagt ccagttttct ttaaaagaag gaatcaaaaa
    14941 acaagtagtg atcggtggga ttgctgtagt ttttgaattg atgggcatca ttttagcagg
    15001 attttcttat gtccttttat gtagtattgc ctatcttcct ggtttttgct tctactggat
    15061 cgcttgtcgt gaaaccaaac atcaaattac gggaaaagaa aaagtaatga ttgggctgat
    15121 ttgtgtggcc gcactgcttt caatcgggtt gcttgctgta ggctggatca agatataagg
    15181 gggaaagaaa catgaaaatc gcaaatttcg gtgtagaaga atggctaaat aaatgggaaa
    15241 agaaagcggt atatgatatt gcacaaagct cgatcgaagc attgacatta gacgaactcg
    15301 ttggcttaga tgggactagc gtaagtaaat ttttcgaaaa acgcaaaaac gaaccattgg
    15361 actatggctg gatcgaagga tcagatgttt tcaaacaaga agtcgcttct ctttatcaaa
    15421 ccgtcgatcc ggaaaacatc ttacaaacga atggagcgac tggtgctaat cttttagctc
    15481 tatacgcgtt agtggaagca ggagaccatg ttgtttcgat gcttcctact tatcagcaat
    15541 tatatgacat ccccaaatct ttaggtgccg ctgttgactt tgttcatttg aaggaagaag
    15601 aagattggca gtttgattta gaacagctag aagccttagt caaacctgaa acaaaaatca
    15661 tctgtttgaa tagtgcaaac aatcctacag gcacattgct agatcggaaa actttagaaa
    15721 aaatcgcaga aattgctcgt acagtagatg catatgtatt gatagatgaa gtatatgcgc
    15781 ctttaacgga taaaggtgaa ttcttgtcga tcgtagatgt ctatgaaaaa ggtatagcaa
    15841 ccaattccct atccaaaacc tactccattc caggtattcg tattggctgg acagctactg
    15901 gacctgagtt ggcagaagtt tttcgaaagt atcgagacta cacgatgatt tgcggcggag
    15961 tcctgtcaga tgatcttgct gtacatgcat tgaagaataa agaaaaaata ttggcgcgaa
    16021 acaaaaagat cattacagag aaccttgtga ttttaaaaca atgggtagcc aatgaaccga
    16081 aagttgaact tgtcgctccc aattacgtct ctacttcttt cattaaatta gccatcgaag
    16141 aagatgacca gacattttgt atcaatttat tagaagaaac aggagtacta ttagtaccag
    16201 gaagtgcctt tgatttaccg aagcatgccc gactaggtta ctgttgtaaa aaagaaacct
    16261 tggaaaaggg attattcctc ttatctgagt tcttgaaaaa acaggcctaa acaaaaacac
    16321 tgcaaggaca gtctgtgaca actgttcaag cagtgttttt catttacgct tattgtctat
    16381 gagaaggaga gtgccctgaa gctatggaga ctcctgaagc tttttcttct gcagtttctt
    16441 gctgatggat ctcttgtgta ttgtgtcgta caagcaagaa gttgaccaaa acaagaagag
    16501 aagtcgaaag aaagacgccg cgataaccaa atgcactgga aacactggag ccgatcatag
    16561 gccctactac gttaccagtt gcttggaaag attggttata actgaagatc cgtccagcag
    16621 cttgatgagg cgaatatttt gtgatcaatg cttggacagc agggagcaga caagcatcag
    16681 agataccaat caaaaaacgt aaggcagcta attgccaaac attcgtgacg aatgccattg
    16741 ggatataaac cagaatcgca aaacccaaac caatcgcaag tattctttca ctcccaatct
    16801 tgtcacctaa gcgaccaaat cttggagcgg ctatcaaagt ggcaatccca ggtatcgaag
    16861 caatgattcc gctgatcaac gtaacattcc catgtccatg cagcaattgg cgaatgtaca
    16921 gactgatgat tggactaatg gaattgttgg atgcttggat gatcatcgtt gtgacgaaca
    16981 taccgatgac gacgtgtgga tatttcaagt ctttgaaaat ctgtttggca gaaaccatgt
    17041 tttctttttt cactgggaca aatttttcat ggacgaaaaa cagactcaac aagaagacga
    17101 aaaataagat gatccctgta atgaaaaagg tagggcgata gccaaacgct gaagcagaga
    17161 tcccgccaag aagcggtcca agtaatgttc ctgtaacaga accagttgct aaggttccaa
    17221 gaacttgacc acttttttct tttggcgtcc cagttgcgac aagggcagtg gcattactga
    17281 tataaccaga aaaaatccct tgaagcagac gcaatgcgac taattgatac acactagtca
    17341 cgcaacccat caaagaaatg acgattgcca ttcctaatga agcacgcaac agcatcaatt
    17401 ttcttccttt ttggtcggct aattttcccc accaaggaga gataagcgta gtcaccaaga
    17461 aagtagatga aaaagtcaat ccgctccaaa agttcaattg tgacgtacta taattaccta
    17521 acgtatctat gtaaagtgac ataaaaggca tgactaaact aaatccgata ccagccataa
    17581 atgttccaaa ccataagaca aacaagtttt gttcccaagt ttctcttttc ctaaataatt
    17641 tttcctttat tcttgcagcc aattagactc cttctttctg aaaattttca tctacagctc
    17701 tttattataa actacaatat gtagtttatc aatgaaagtc tcttttttct ctttcttttt
    17761 cgtggtaaac tgaaagagtg aaggatttat tttatgagag gaaacatgga aatgaaaaaa
    17821 agaaatctgt ctcaagagaa aatcatcgac tgctttcgag aactagcaga agagatggat
    17881 gttcagcaaa taacctttca gcatttagcc aaacgcttgg atatcaaggc tccctcgctg
    17941 tataaccatt tcaaaaatat tcgtgatgtc aaaacagcgc taacagcgaa acttctgcag
    18001 gaacttaatg accagctgcg acgggctctt gtgggaaaaa gtggtcagga agcactgcag
    18061 atctatgcag aaacttatcg atcctttgct ttttcaaatc aagcagtgta tgagttactg
    18121 atcagtgtac cgcataccaa tgaagctgtt ttattagatg gaatccatga aacgaatcag
    18181 atcattttgc agatatttga ggcttttcaa ttaacgaaag aagaaaaagt tcatcgaagc
    18241 agggaacttc gtagtatgat ccacggttat ctttccttac gttttttagg atactttaca
    18301 aaagaaccaa caatcgatcc agaagacagc tatttttgga tgatcaataa ctttatcgct
    18361 actttgcctt aaacaatcag attcatttgt aacaatttta agatgttcat taaatgaccc
    18421 ttttataaga gaaaattctt gcaactttct tgaaaagtga ctaagatgag tctagaaaag
    18481 ctgaaagaag gagcacgagt atgtatcaag taattaccat gttcggggac aatgagcctt
    18541 ggtggttttt tgaagactgg gaagaagata ttgaagaaga agaaacattc gagtctttcg
    18601 aagaagcacg acaagcgtac gaaaaacaat ggaacgaaat ccatcaaaat tatgaatata
    18661 tcaatgcaaa aagtaacttt ctcagcgcct tttggaatga cggagatgag cgctggtgtg
    18721 aagagtgtga cgatgattta cagcaataca agggacttgc gttgttgaaa aatcatcagc
    18781 ccatcacagt agaaagcaga aaggaatttt atgaaacaac taattctagc ggaaaaacca
    18841 agcgttgcga aagacctaag caaggtgctt ggtgctaatc aaaagcacaa aaattattat
    18901 gaaggaccca aggtgatcgt tacttgggcc cttggtcatt tattaggatt gaaaatgccg
    18961 gaagatctga ataaggaatg gcagagctgg caaatggaca cgttgccaat gatccctaaa
    19021 aaactaggaa tcaaaccact tcctaaaaca ggacatcaat taaaagcaat caagcagctg
    19081 gctcagcgaa aagatgtctc agaagcagtc attgcgacag atgccggaag agaaggggaa
    19141 cttgttgctc gctggatctt agagtatgtg catttcaata aaccagtgaa gcggttatgg
    19201 atctcttctc aaacagataa agcaatcaaa actggattca agcaactaag acctgcgaaa
    19261 gaatatgatg cattgtatga atctgctctt gcccgggcaa aagcggactg gctggtagga
    19321 ttgaatgtga ccagagcctt aactgtgaag tacgcggata atttatcagc aggccgtgtg
    19381 cagacaccga cactagcatt agtgagagac caagaaaaga aaatcgagca gttccgtcca
    19441 caaacgtatt tcacgatcac gttgcaagct ggaaaagaac aagccaaaat gcttcaaaaa
    19501 aatcaatttg ctttaaaaac acaagaagaa gcacaaaaac ttgttcaatt gatcagtaca
    19561 agcaaaggaa ctgtcacaga agtccaagaa aaattaaaaa cggaatctgc gccattgcct
    19621 tatgatttaa cagagatcca aagagaagcc aatcaacgct atggctattc tgcaaagaaa
    19681 acattaagtc ttgtacaaag tttgtatgag acccataaga tcgttactta tccgcgtaca
    19741 gacagcaaat atctgacaaa tgacatgaaa agtacgatga aagaacgatt gcaagcagtc
    19801 agcgactttg ctcctgaggt caagcagtat ttgaaaaacg gagccatcgt ccaacagaca
    19861 aaagtattcc aagacagtaa agtgaccgat caccatgcac ttttgccaac tgaaaacaga
    19921 gcacgttatg aaaaattaag taatgaagag cgaaaaatct atcaaatgat tgtctctcgt
    19981 tttttaggac tgtttgctca accccataaa gtcagccaaa caaaagttac ggttgaattt
    20041 ggtaaagaac gattcatttt tcggcaaaat cgtgtcgtcc aagcaggatg gaaaggcgaa
    20101 actgaaactg agacggaaac agtcaaatgg gaaaaaggaa tgagaatcaa tcccgatttc
    20161 gccatcaaaa aagaactatc tgcaccgcct aaaccattgt cagaagcaag cttgcttggt
    20221 caaatggaaa aatacagtct gggaacccca gctacgcgtg cagagattat cgagaaacta
    20281 atcaaatctg aactgatgga acgtacccct caaggactgc aagtatctcc taaaggtaag
    20341 caattattag tattggtaaa tccctcttta gtaacaccag atttgacaga aagctgggaa
    20401 aaagaccttg aagctattgc tgcaggaaag aaacaagcag gaaccttctt gaatggaatc
    20461 gaaaaagaaa caaaacgctt ggtgaatgag atcaaatcaa gcaaacaaga atatcaagat
    20521 ttctcgatta cccaaaagaa atgcccagaa tgtggggcca atctgcgcga gaaaaacaca
    20581 cgtgacggca aaatctatgt ctgcaccaat tcagattgca actaccgcag aagaaaagat
    20641 ccaaaagtct ctaatcatcg atgcccgcaa tgtcaccgga aaatggaaat catcgaaggt
    20701 aaaaacggtg cttatttccg ttgtaaatac gatggaacga cagaaaaatt gcttgataaa
    20761 aaagagcaga agaagaaaat gaccaaacat gaagaacgac gcttgctgaa aaaatatgca
    20821 caagcagaag aaccagaaga aagcccatta gcagcagcac taaaagcagc aatgaagaag
    20881 taataagata tgaaagaggt tgtga
//
LOCUS       DAZKLD010000041        20553 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.041, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000041 DAZKLD010000000
VERSION     DAZKLD010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20553)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20553)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20553
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.041"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(195..560)
                     /locus_tag="ACG8KR_001206"
     CDS             complement(195..560)
                     /locus_tag="ACG8KR_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0200589.1"
                     /translation="MIKTCWKNLPLLLSFVPYVHFALLLDFRYHSVSGFITLIFLSLF
                     AGYYFQRNRRIISLFIANIISTVTSYLFCANFTEWRYFYHPLKPTQLILLLAGIYLVP
                     QILGSLWAVALSYKKARHP"
     gene            768..1250
                     /locus_tag="ACG8KR_001207"
     CDS             768..1250
                     /locus_tag="ACG8KR_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0200590.1"
                     /translation="MRRKAVIDRLYLFFWFFPIVLLPFTWVNAVVQGAEILGLIQGSG
                     LVVSRNFPALTVFYLVISVGLLLPKSLINHYNWQLLYFSCLLVFTSMFSLLPRLIIGP
                     NGLSESGLKDSAYSSMYFLTIKPWFYLVIASFILATFFYFWTEIRIKSERSVSIKNNY
                     "
     gene            1447..2337
                     /locus_tag="ACG8KR_001208"
     CDS             1447..2337
                     /locus_tag="ACG8KR_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS family protein"
                     /protein_id="HFQ0200591.1"
                     /translation="MKKVLFTLGMMGILLAGCGSGSGNTDGSATQNNSSDSNEQVKIV
                     AVGSTALQPLVDAAQESFVQENPNYQISVQGGGSGTGLSQVEAGAVTIGNSDVFAEER
                     DGVDASKLVDHKVAVVGMAPIVNKDTDVKDITKQELIDIFTGKITNWKEVGGKDQKIN
                     VVNRANGSGTRATFEKWGLDGATPVQSQEQDSSGTVRQLVSQTPGAISYLAFSYLDDS
                     TQALSIDGVEPKEENVADNSWGIWSYEHMYTNGKPSPEVQKFLDYMMTEEIQEGPVKE
                     LGYLPITMMEVERDHEGNIK"
     gene            2663..5137
                     /locus_tag="ACG8KR_001209"
     CDS             2663..5137
                     /locus_tag="ACG8KR_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFQ0200592.1"
                     /translation="MKNKWINRSKKKKVTQRSSDKVIRLFPSYKEGLTADQVKERIEK
                     GAANNSVDPTFKTNQQIVLENIFTYFNLIFLILAILLCLVESYKNLTFLPVIIANTGI
                     AIFQEIRSKKILDELNVLNTAKVNVIRDGIEQTIDIEDLVIDDIVILETGHQIPADAE
                     VVEGKLQVNEALLTGEADEITKEIGDPLMSGSFIVSGKAYARLDKVGADSYISQLTVK
                     AKTMGEGEQSEMIASLNQLIKWIGIIIIPIGILLFSQSYFFNGNTIKESVVAMEAALI
                     GMIPEGLYLLTTIALALSATKLAKQRVLLHNMKSIETLARVNVLCVDKTGTITENKMS
                     VQKVIVSKKQEIISQSDQLEACIADYAKAMAGDNATMEAVKAYFTKTTDQSYHHVIPF
                     SSVQKFSSVALADKVYVLGAPEMVLRDQLPEYAEEFVSFAEKGYRVLVFGVYDGILEE
                     PVLTEAVTPLGYILIANPIRKEARATFDYFKKQQVAIKVISGDNPLTVSNVAVQAGIS
                     HADQYVDASKLSEEEYEAAVEKYTVFGRVKPEQKKIFVQLLKRRNNTVAMTGDGVNDI
                     LAMKEADCSIAMASGNEAAMQASQVVLLDSDFSRMPEVVAEGRRVVNNIERSASLFLV
                     KNIFSFLLSLFSVIFALTYPLEPSQITLISLFTIGLPSFLLALEENKKRIRGKFIMNV
                     MEKAVPGGLTDMIVVGALVICGVTLNLNKTDVSTASTMLLIAVGFLVLYKICSPLNKF
                     RSQIILFCASGNFFSVIFLHKLFSITGISAVSLLLLSILFFSADSIFRHLTTLVEYLF
                     ERKNEKEQKPFSRVVKKFLSIFKREN"
     gene            complement(5240..6976)
                     /locus_tag="ACG8KR_001210"
     CDS             complement(5240..6976)
                     /locus_tag="ACG8KR_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFQ0200593.1"
                     /translation="MKWKKIYERLFFLILLVSLFFGGWQLISHYFQQQIVEQQESYLT
                     KKAQLLIRQLDVENLQAAQNKTALEEFVHQSNERITLMDATGKILFDTNNETLNEQRN
                     SRPEIKAVLNGGNLGSALRKSTTLNGDLLYVALPVKKSGQLEAILRIAEPTSGFLPRT
                     ESFRRWVFFFFLSFFLVLAGMIYYLIYQKNQPLKTVLPVLKKMVQNPNQAEVIMQTPD
                     QWEELYQTINALSEQMSKMYRAYTTTEEQLYTLLNELMIGVFLIDDADSRLLLLNPKM
                     QLHLNVVSYQPEQRYTEVIQEPKLIQLIHQVTPEHPLIHQEITLTEGRQTLDLSLRYF
                     TNSEGTGQILGVAYDLTKVRRLEKLQKDFVGNVSHELKTPVTSLIGFTETLLDGAKDD
                     PQTLTSFLEIMQKDAIRLDKLIREIIQLSKDGENSYEIQTIYTEPYFQQIVQNYQPII
                     EKKRLTIRLIGKNEPFTTKTDILYPIIKNLIENAVQYSKSDSEIIIRYQATDDLSFSV
                     QDFGIGIDIEDQERIFERFYRVDKARSRHSGGTGLGLAIVKDYVQLLHGTITVDSHLG
                     TGSTFTVTIPKM"
     gene            complement(6973..7677)
                     /locus_tag="ACG8KR_001211"
     CDS             complement(6973..7677)
                     /locus_tag="ACG8KR_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFQ0200594.1"
                     /translation="MKKILVVDDEPSIVTLLTFNLEKEGYKVTSATDGGEGLELALEQ
                     SFDFIILDVMLPTMDGMEITQRLRQEKNETPILMLTAKDDQVDRIIGLEIGADDYLTK
                     PFSPREVLARMKAIFRRIEPRKQSKDEAEPEYLSIGQITADLTNYQVTVDDHLIELTP
                     KEFELLVYFMKRKDRVIDRDTLLDRIWNFDFAGQSRIVDVHVSHLREKIERDPKHPKY
                     LVTIRGFGYKFQEPKR"
     gene            7808..8311
                     /locus_tag="ACG8KR_001212"
     CDS             7808..8311
                     /locus_tag="ACG8KR_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HFQ0200595.1"
                     /translation="MIGISACLGGLACRYDGQKKEVPELMELVETGEAVMVCPEVIGG
                     LPIPRDPAEIIGGNGFDVWKDQAKVWTTSGEDVTEAYKNGAIKAYQKLQEKHIDLVIM
                     KEKSPSCGTHSIYDGSFSGIKKNGAGVATAYFIQQKMTVLSDEEWLKTRGEQNGNRKD
                     QSNERAL"
     gene            8271..8609
                     /locus_tag="ACG8KR_001213"
     CDS             8271..8609
                     /locus_tag="ACG8KR_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HFQ0200596.1"
                     /translation="MEIEKTNRMNALFEFYSTLLTEKQMNYMELYYADDFSLGEIAEE
                     YEVSRQAVYDNIKRTSKILEDYEKKLHLFSNYIVREQVLENMTAYITDKYPEDKKLLE
                     YVQQIQAIEE"
     gene            8630..10048
                     /gene="ffh"
                     /locus_tag="ACG8KR_001214"
     CDS             8630..10048
                     /gene="ffh"
                     /locus_tag="ACG8KR_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HFQ0200597.1"
                     /translation="MAFESLTERLQQAMGKIRKKGKVSEADVKEMMREIRLALLEADV
                     NLQVVKDFTKRVRERAVGAEVLESLSPAQQIVKIVDEELTITLGSETAELNKSPKIPT
                     VIMMAGLQGAGKTTFTGKLANYLKKNENARPLLIAGDVYRPAAIDQLKVLGQQLDVPV
                     FDMGTEVSPVEIVRQGMELAKEKKNDYVLIDTAGRLHIDETLMDELKQIKELTQPNEI
                     LLVVDAMTGQDAVNVADSFNQQLGITGVVITKLDGDTRGGAALSIRSVTGAPIKFIGS
                     GEKLTDLEVFHPDRMASRILGMGDMLTLIEKAQQDYDEKKAEELAQKMRENSFDFNDF
                     IEQLDQVMGMGPLEDLIKMIPGMNQVPGIENVKVDPKDVERKKAMVYSMTPAERANPD
                     LLNPSRRRRIAAGSGNSVVEVNRMIKQFKESRKMMQQMSKGDMNIPGMDQMFGSGVKG
                     KLGKMAMNRMIKKNKKKKKKRK"
     gene            10160..10738
                     /locus_tag="ACG8KR_001215"
     CDS             10160..10738
                     /locus_tag="ACG8KR_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HFQ0200598.1"
                     /translation="MRCPWSEQTESMRQYHDTVWGVPEYDDQKLFRKLMLDINQAGLS
                     WQTILNKSEAFDEAYDRFDIKKVANYGETKISELMENKGIIRNRRKIEAAVQNAKAVL
                     KIQEEIGSFSEYLWSFSNGKIVDSSYHSQEEVPTTSPLSDELAKDLKKRGFKFIGSTT
                     IYAFLEAVGIINDHLDSCFRKEEVVRIGRKGE"
     gene            10742..11263
                     /locus_tag="ACG8KR_001216"
     CDS             10742..11263
                     /locus_tag="ACG8KR_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HFQ0200599.1"
                     /translation="MRKIIFYGAISLDGYLATKTDDLQWLFDTPTGKQTTYEAFYETI
                     DTTIMGRKTYQEAKKYLETEAIYPEKKNFVFSTHAQLQLPDAEVVNEDPVAFLKNLQM
                     TEGKDIWVVGGGRLIKPLLEHQMIDEWYIQITPVLLGDGIPLFQKGTYEARFEMLDTT
                     RFGEFIELHYVRK"
     gene            complement(11342..11896)
                     /locus_tag="ACG8KR_001217"
     CDS             complement(11342..11896)
                     /locus_tag="ACG8KR_001217"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HFQ0200600.1"
                     /translation="MTKKIGFFIGSLRKDSYNKKVAEAFAKLLPEGYESVFVKIDDLP
                     FYNEDLETPEQAPAEWTRFREEVRGLDGVVFVTPEYNRSVPAVLKNALDVGSRPYGHS
                     VWDHKPGLVVTASPGGIGGFGANHHLRQSLVFLNVPTLQQPEAYIGNVANLIDENGNI
                     VEGTLSFFQTILDAYLDFSNKLTE"
     gene            12149..12424
                     /gene="rpsP"
                     /locus_tag="ACG8KR_001218"
     CDS             12149..12424
                     /gene="rpsP"
                     /locus_tag="ACG8KR_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HFQ0200601.1"
                     /translation="MAVKIRLKRMGSKKSPFYRIVVADSRSPRDGRFIETVGTYNPLK
                     DPAEVVLKEDLVLDWLSKGAQPSDTVRNILSKEGVMQKHHEAKFSKK"
     gene            12436..12687
                     /locus_tag="ACG8KR_001219"
     CDS             12436..12687
                     /locus_tag="ACG8KR_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HFQ0200602.1"
                     /translation="MKDLSDLVLTIVRPLVTYPDQVKLDVVESKDFYEYNLTVAPEDI
                     GRIIGKQGRVAKAIRTIVYGVRINAPKKVRLNIIDDKEK"
     gene            12811..13602
                     /locus_tag="ACG8KR_001220"
     CDS             12811..13602
                     /locus_tag="ACG8KR_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0200603.1"
                     /translation="MRKKMLFIPFALLLSACSSVTQESDNTHLSSSISETVTSPEKSS
                     TKTATTTEQTMATSNNEKKTALDQLKEQQPNVPMPLDVPVSSGYLNIAATHTKQGYSI
                     LYYRTDRPLGLNADELNQETPIATYLYQYGFASSQETIQVLQPFEIDTNGQQVDLGSR
                     ITGYQQGAAGSSFLEWQEGNWRIRIRGNNIEGQDPLLLAKEIVAYLEENSLPAPEQFG
                     KITVDMGDTTNRAVEVSWQEPKNAYTITHQDPMSALKMAVSMKRL"
     gene            13772..14293
                     /gene="rimM"
                     /locus_tag="ACG8KR_001221"
     CDS             13772..14293
                     /gene="rimM"
                     /locus_tag="ACG8KR_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319275.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HFQ0200604.1"
                     /translation="MTEYLNVGKIVNTQGIKGEVRVISTTDFPEERYKKGTVLTLFQD
                     GKAPVELTVKSHRKHKNFDLLSFEGHPSINDVEKYRDGILKVSKDNSVDLAENEFYYH
                     EIIGLHVLEDGTELGKVKEILSPGANDVWVVQRPKKADILLPYIASVVKEVDLEAGVV
                     RVEIPEGLIDDED"
     gene            14283..15044
                     /gene="trmD"
                     /locus_tag="ACG8KR_001222"
     CDS             14283..15044
                     /gene="trmD"
                     /locus_tag="ACG8KR_001222"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HFQ0200605.1"
                     /translation="MKIDVLTLFPRMFEGPMGESIIGKAVDKGLLDINISNFRDYSDN
                     KHQTVDDYPYGGGAGMLLKVQPIYDNIQAIEKAAPDVKKRVILLDPAGKRFDQKMAEE
                     FSQEEHLVFICGHYEGYDERIRTLVTDEVSLGDYVLTGGELGAMVMIDATVRLLPDVL
                     GNQTSAQTDSYSTGLLEHPQYTRPAEYKGMKVPEVLTNGNHKLIEEWQLKESLRRTYQ
                     RRPDLLETLEWTPQMTKFLEEIKKEEQEKTADEAK"
     gene            15180..15527
                     /gene="rplS"
                     /locus_tag="ACG8KR_001223"
     CDS             15180..15527
                     /gene="rplS"
                     /locus_tag="ACG8KR_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HFQ0200606.1"
                     /translation="MNPLIEELTKEQLRSDIPAFRPGDTVRVHAKVVEGTRERIQLFE
                     GVVIKRRGAGISETYTVRKVSNGVGVERTFPLHTPRVAKIEVVRYGKVRRAKLYYLRA
                     LHGKAARIKEIRR"
     gene            15837..16079
                     /locus_tag="ACG8KR_001224"
     CDS             15837..16079
                     /locus_tag="ACG8KR_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HFQ0200607.1"
                     /translation="MNDGKVKQVPSSTKKKNILLKEVLKRFDHGVTYTETEVNSILLN
                     VFSSGDYVEQRRYLITFGFFKRSSDGRAYQMMGIEN"
     gene            complement(16167..16313)
                     /locus_tag="ACG8KR_001225"
                     /pseudo
     CDS             complement(16167..16313)
                     /locus_tag="ACG8KR_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332517.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamate
                     endopeptidase"
     gene            16370..16684
                     /locus_tag="ACG8KR_001226"
                     /pseudo
     CDS             16370..16684
                     /locus_tag="ACG8KR_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345994.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronyl hydrolase"
     gene            16713..17972
                     /locus_tag="ACG8KR_001227"
     CDS             16713..17972
                     /locus_tag="ACG8KR_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFQ0200608.1"
                     /translation="MKRWKKMMIGSIVLGMSLVFGACGNQEDSSEESANEITVSTWNY
                     ETTPEFKALFEAFEKKTGIKVKAVDIASDDYDTKLTTMLSSGDTTDVLTMKNLLSYSN
                     YALRDQLVDQTERIRELDTDAAEETYEMYDIDGKTYALPYRTDFWVLYYNKKMFDDAG
                     IEYPKNLTWDEYEDLAKKLSKNDGQVYGAYQHIWRSTIQAIAAAQNEANLVEPDYQYM
                     TDYYDRALRMQKEGAQMDFGTAKSTKVTYQSQFEEQKAAMMYMGTWYMAGILANKEAS
                     KTEVEWGITAIPQKKKGESVTTFGSPTAFAVNKNSKKQKAAQEFIEFAASEEGAKVLA
                     GVGVVPSYRTDEIDQLYFNLAGMPTDEISKKAFSPDEIKLEFPIDTYGPAIDKILQEE
                     YDLVLVGDETPEKGTVNMEKRVAAEKE"
     gene            18025..18921
                     /locus_tag="ACG8KR_001228"
     CDS             18025..18921
                     /locus_tag="ACG8KR_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0200609.1"
                     /translation="MENSINKKATNHLRRKRTWIAYSFIAPNFIGFFVFTLIPILFSL
                     VLAFMKWDSFSTPEFAGLDNFRKMANDETFWISLKNTFLYTAAVVPGTLVCSLGLALL
                     LNQKIRGMKLFRTIFFFPYVTSLVAIAVVWNMLFHPTVGPINIFLRRFIEHPPGWTSS
                     SNWALWAIVIVSIWRGMGYYMILYLAGLQSIQKELYEASSLDGANRWKQFLNVTLPSL
                     RPTTFFVTIMLVINCFKVFDLVQVMTDGGPGRATNVLVYQIYNEAFKKFDFGYASAIS
                     LVLFVIVLGVTVVQFSWNKRRT"
     gene            18932..19801
                     /locus_tag="ACG8KR_001229"
     CDS             18932..19801
                     /locus_tag="ACG8KR_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0200610.1"
                     /translation="MEESVRNERKKAKIRSKNMPFIQWLGMILLIVLALLTIIPFLWM
                     VSASFKANNEVLTIPIQWIPKTWHLENYLVIWSRIPLVTFFKNTAFLSVVITLIQLFT
                     SSFAVYGFSELNFKGRDILFLAYIGTIAVPWQSYMIPQFIMMRKVGLTDTLWSLVLLQ
                     AFNAFGVFLLKQYYSSIPDSLCESARIDGLSEWEIYRKIILPLTKPAIASLTIITFVN
                     TWNDYMGPFIYLSSTENKTIQLGLKMFIGLFDAEYALIMAASVVSVLPVAFIFLAMQK
                     YFVEGIASSGMKE"
     gene            19811..20486
                     /locus_tag="ACG8KR_001230"
                     /pseudo
     CDS             19811..20486
                     /locus_tag="ACG8KR_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334179.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
BASE COUNT         6776 a         3559 c         4230 g         5988 t
ORIGIN      
        1 atgtttccac ctcaaattca atatctatta ttggaatcat tattgctgca attattacac
       61 aactgttttt agtgtttgga gagaagaaaa gaagtaaaaa atagaaaaac tagtgatagt
      121 tttggtgata taagaaaaac atatatgtat aaaaagaaga aagtagcttc tctttgtaca
      181 tatatggttt gtttttatgg atgccgagct tttttataag ataaggctac cgcccataac
      241 gacccaagga tttgtggaac taagtaaata cctgcaagaa gcaagatcag ctgggtcggt
      301 tttaaaggat gatagaagta acgccattca gtaaaattgg cgcaaaataa ataggaagtg
      361 acagtagata taatattggc aatgaataaa gatataatac gcctgttcct ttgaaaataa
      421 tatccagcaa aaagagataa aaatatcaat gtgatgaacc cgcttacgga atgataacga
      481 aaatcaagca gtaaggcgaa atgcacataa ggaacaaaag ataaaagcaa cggaaggttt
      541 ttccagcaag tttttatcat ctttcatcac cacctaacaa gtgttattat tttgttagtt
      601 ttattgtaaa ttgaatcctg caataatcaa ttactttttc attatttcta gattaaatga
      661 ataaactctc atgccattat ctctaaaaaa aaagtttttc cccctaattt ttttagatag
      721 aaaattgtat agtatgatga gagaaaaagt agtttggggg aacgttaatg agaagaaaag
      781 cagtaatcga tcggctttat ttattttttt ggttttttcc tatcgttttg ctacctttta
      841 catgggtaaa tgctgttgta caaggtgcgg agatattagg cctgatccaa ggaagcggat
      901 tagtggtttc taggaatttt ccagctttga ctgttttcta tttagtgata agtgtaggtc
      961 tcttattacc aaaatctctg atcaatcact acaattggca gctgctttac ttttcttgct
     1021 tattagtatt tacatctatg ttttctttgc tgcctcgctt gattatcggc cctaatggtt
     1081 tgagcgagag tggattgaag gattcagctt attcctccat gtatttcttg acgatcaaac
     1141 catggttcta tttagtaatt gcctcattta ttcttgcaac atttttttac ttctggacag
     1201 aaatcaggat aaaaagtgaa cgatctgtat ctataaaaaa taactattga tggtggatca
     1261 tcagtaaaaa agcatatgag ctgcgtaaaa tgcatgtttc tcatatgctt tttttattat
     1321 tatgtaaaga atgcgaaaaa cgtttacatg atttttacat aacttaacta tatttttaca
     1381 gcaaccatac gagggattta cagtcgactg ataaacttta gttgtaaata agaaaggagc
     1441 aataaaatga aaaaagtatt gtttactcta ggtatgatgg gaattctttt agccggatgc
     1501 ggctcaggat caggaaatac agatggctct gccacacaaa ataattcaag tgactcaaat
     1561 gaacaagtaa aaatcgttgc ggtaggttcg acagcattac agccgttagt cgatgcagca
     1621 caagaaagct ttgtacaaga aaatccaaat tatcaaatct ctgtacaagg aggaggaagc
     1681 ggaactggtt tgagccaagt cgaagctgga gcagttacta tcggaaactc tgatgtattt
     1741 gcagaagaaa gagacggagt tgatgcttca aaattagtgg atcataaagt agcagtggtc
     1801 gggatggcac caatcgtcaa taaagataca gatgtcaaag atatcaccaa acaggaattg
     1861 attgatatct ttacaggaaa aatcactaac tggaaggaag ttggcgggaa agatcaaaaa
     1921 atcaacgtcg tgaaccgtgc aaacggcagc ggtacccgcg cgacattcga aaaatgggga
     1981 ctggatggtg ctacccctgt gcagtcccaa gaacaagatt catcaggaac agttcggcag
     2041 ctggtaagtc aaacaccagg agcaatcagc tatttagctt tctcatatct cgatgattcc
     2101 acacaagcac taagtatcga cggtgtagaa ccaaaagaag agaatgtagc agataacagc
     2161 tggggaatct ggtcatatga acatatgtac acgaacggaa aaccttcccc tgaagtccaa
     2221 aaattcttag actacatgat gacagaagaa atccaagaag gacctgtcaa agaattgggc
     2281 tatttaccaa tcacaatgat ggaagtagaa cgtgatcacg aaggaaatat caaataacta
     2341 gcagataaac atgccacggt agtcgttact attaaggaaa aatcaactat gtttccccac
     2401 atatacattt ttcctttaat tgtgaaaaat agttcctcat attttgccgt tttttgagct
     2461 aataccgagg gtcagctgat tgaacaccgt ttattcgaaa aaaagagcta cgacatagaa
     2521 ctgtcgtagc tttttttttg ctatatgaca ggaatgaaag gtgtttgcaa gcttaatgtg
     2581 aggaacactt tccatatctg ttctatactg gataggagaa gtttggaaat atccagaaaa
     2641 cgaaatcaat cgaggtagcc acatgaagaa taaatggata aatcgaagta aaaaaaagaa
     2701 agtaacacaa agatcatcag ataaagtcat ccgtcttttt ccatcgtata aagaaggatt
     2761 aacggcagat caagtcaagg aacgaataga aaaaggtgca gcaaataatt cagtcgatcc
     2821 cacattcaaa acaaatcagc aaatcgtatt ggaaaacata tttacgtatt tcaatttgat
     2881 ctttcttatc ttagccattt tgctttgttt agtagaatcc tataagaact tgactttttt
     2941 accagttatc atagccaata caggaatcgc catttttcag gaaattcgat ccaaaaagat
     3001 cttggacgaa ctaaatgttt taaatacagc aaaagtcaat gtcatacgag acgggataga
     3061 acaaacaata gatatagaag accttgtaat agatgacatt gttattttag agactggaca
     3121 tcaaattcca gcagatgcag aggtcgtgga agggaaactg caagttaatg aggctttatt
     3181 gacaggcgaa gcagatgaaa taaccaaaga aataggcgat ccattaatgt ccggcagttt
     3241 cattgtttcc ggaaaagctt acgcccgatt agacaaagtt ggagcagatt cgtatatttc
     3301 ccagctgaca gtcaaagcca agacaatggg agaaggagaa cagtcggaga tgattgcttc
     3361 tcttaatcaa ttgatcaaat ggattgggat catcattatt cctatcggta tcctgctttt
     3421 ttctcaaagt tactttttta atggaaacac gatcaaagaa agtgtcgttg caatggaagc
     3481 agcattgatc ggtatgatac ctgaaggact gtacttgctg acgacgattg cattagcatt
     3541 aagtgcgact aaactagcaa agcaaagagt tcttttacac aatatgaaaa gcattgagac
     3601 attggcaagg gtaaatgtcc tttgtgtaga taaaacagga acgatcacag aaaataaaat
     3661 gtctgttcaa aaagtgatcg tctctaaaaa acaagaaata atcagtcagt cagatcaatt
     3721 ggaagcatgc attgctgact atgcaaaagc gatggcagga gacaatgcaa caatggaagc
     3781 tgttaaagca tattttacaa aaaccactga tcaatcctat caccatgtta ttcctttttc
     3841 ttctgtccaa aaattcagca gtgtagcgtt ggcagacaag gtgtatgttt taggagcgcc
     3901 cgaaatggtt ttgcgcgatc aattgccgga atatgcggaa gagtttgttt ctttcgctga
     3961 aaaaggctat cgcgtgttgg tctttggtgt gtacgatggg attcttgaag aaccagtatt
     4021 gacagaagca gtcacaccat taggctatat tctgattgct aatcctatca gaaaagaagc
     4081 aagagccaca tttgattact tcaaaaaaca gcaagtagcc atcaaagtga tttctggaga
     4141 taatccgctc accgtatcca atgtagctgt ccaagcaggg atttcacatg ctgatcagta
     4201 cgttgatgca tctaaattat cagaagaaga atatgaagca gctgtcgaaa aatatacagt
     4261 ttttggacgc gtcaagccag aacaaaagaa aatcttcgtt caattgctaa agcgtcgtaa
     4321 caatacagta gctatgacag gtgatggcgt gaatgatatt ctagccatga aagaagcaga
     4381 ctgcagtata gcgatggctt ccggaaatga agccgccatg caggcctcac aagtggtctt
     4441 acttgattct gacttttcac gcatgccaga agttgttgca gaaggacggc gcgttgtgaa
     4501 caatattgag cgatcagcca gcttgttttt agtgaagaat attttctcct ttttgctctc
     4561 actgttttca gtgatctttg ctctgaccta tcctttagaa ccatcacaaa tcacattgat
     4621 tagtttgttt actatcggac ttccatcttt cttgcttgct ctagaagaaa acaaaaagcg
     4681 aatcagaggg aaattcatca tgaatgtgat ggaaaaagct gtgccaggtg gactaacaga
     4741 tatgatcgta gtcggtgcgt tggtgatctg cggggtgact ttgaatctaa ataaaacaga
     4801 tgtttctaca gcatctacca tgcttttgat tgcagtaggg ttcctcgttc tctataaaat
     4861 ctgttcaccg ctgaataaat tcagaagtca gatcattctt ttctgtgcta gcggtaactt
     4921 tttttcggtc atctttttgc ataagctgtt ttcaatcacc ggtatctcag ctgtatcact
     4981 tttgctgttg agcattctat ttttctcggc agattcgatt tttaggcatt tgaccacatt
     5041 ggtagaatat ttgtttgaac gtaaaaatga aaaagagcaa aagccgttta gtcgcgtagt
     5101 taaaaaattt ctatcgatat ttaaacgaga aaattgacga aaagtatctc aaaaaagagt
     5161 aaaaaagaac aaccactcgt gtatctttcg aagcaggatg agtcatccaa ctttaaaacg
     5221 cacgagcagt tgctcttttt tacattttcg gaatggtaac ggtgaaagtc gaacctgttc
     5281 caagatgact gtctacagta atcgtaccat gaaggagttg cacgtagtct tttacaatcg
     5341 ctaaccctaa tccggtcccg cctgaatgcc ggcttcgcgc cttgtctacg cggtaaaaac
     5401 gctcaaagat ccgttcctga tcctcaatgt cgatacctat accaaagtct tggacagaaa
     5461 acgatagatc atctgttgcc tgatagcgga taataatctc gctgtcgctt tttgaatatt
     5521 ggacagcatt ttcaatcaga tttttgataa ttggataaag gatatctgtt ttagtagtaa
     5581 atggttcgtt ctttccaatc aatcgaatcg tcaaccgttt cttttcgatg attggctgat
     5641 aattttgaac gatttgctgg aaataaggtt ctgtataaat tgtctggatc tcgtaagaat
     5701 tctcgccatc ttttgacagc tggatgattt cccgaatcag tttatctaac cggatcgcat
     5761 ctttttgcat gatctcaaga aaagaagtca gagtttgcgg gtcatctttt gcaccatcca
     5821 gtaaagtctc ggtaaaacca atcagtgaag tcacaggagt ttttaattcg tgagagacgt
     5881 tgccaacgaa atccttttgt agcttttcta aacgtcgcac tttcgttaag tcatacgcca
     5941 cccctagaat ctgtccggta ccttctgaat tagtaaagta gcgcaaactc aagtctaatg
     6001 tctgccgacc ttctgtaagg gtgatctcct gatggatcag cggatgctca ggagtcactt
     6061 gatgaatcag ctgaatgagt tttggctctt gaatgacttc tgtgtaccgc tgttcaggtt
     6121 gatagcttac cacgtttaaa tgtagctgca tctttggatt aagcagtagt aaacgagaat
     6181 cagcatcatc aatcaggaat acgccaatca tcagttcgtt cagtaacgta tataattgct
     6241 cttctgtggt agtatacgcc cgatacatct tactcatttg ttcgctcaac gcattgatcg
     6301 tctgatacaa ttcttcccat tgatcgggtg tttgcatgat tacttccgcc tgattaggat
     6361 tttgcaccat tttttttagt acgggcagca cggttttcaa tggctggttt ttttgataga
     6421 tcagataata gatcattcct gcaagaacaa ggaagaaaga cagaaagaag aaaaagaccc
     6481 aacggcgaaa actctcagtt ctcggtaaaa aaccgcttgt cggttctgcg atacgtaaaa
     6541 tggcttctaa ctggccactt tttttgactg gtaatgcgac atataaaaga tccccattca
     6601 aagtagtact tttgcgcaaa gcgctaccta ggttcccacc attcaatacg gctttgattt
     6661 cgggacggga gtttcgctgt tcattcaatg tttcattgtt tgtgtcaaat aagatctttc
     6721 cagtagcatc cattaaagta atccgttcgt tcgactgatg gacgaattct tccaaagcag
     6781 ttttgttttg cgctgcttgg aggttttcga catctaattg ccggatcagc agttgtgctt
     6841 tttttgtcag gtagctttct tgctgttcca cgatctgctg ttgaaaataa tgggaaatca
     6901 attgccagcc accaaaaaac aaactaacta aaagaataag aaagaagaga cgttcataaa
     6961 tttttttcca tttcatcgtt tgggttcctg aaacttatag ccaaatcctc taatagtcac
     7021 caagtacttt gggtgttttg gatctcgctc gattttttct cgcaaatgac taacgtgtac
     7081 atctacgatt cggctttgtc ctgcgaagtc aaaattccaa atgcggtcta gcaatgtgtc
     7141 tcggtcgatc acacggtctt ttcgtttcat gaaataaaca agtaattcaa attctttcgg
     7201 tgttagttca atcagatggt cgtctacggt cacttgataa ttggtcaaat cagcagtgat
     7261 ttgtccaatc gatagatatt caggctcagc ttcatctttt gattgcttac gaggttcaat
     7321 tcgtctgaat attgctttca tacgggctaa tacttcgcga ggactaaatg gtttggtcaa
     7381 atagtcgtca gcacctattt ctaggccaat gatccgatcc acttggtcat ctttagccgt
     7441 cagcatcaaa atcggtgttt catttttttc ttgacgcagc cgctgagtga tctccatgcc
     7501 atccatcgtt ggaagcatca catctaaaat gatgaaatca aatgattgtt ctaatgctaa
     7561 ttctaatcct tcgcctccat ctgtggcact ggttaccttg tagccttctt tttccaaatt
     7621 gaacgtcaag agcgttacaa tagaaggttc atcatcaaca actagtattt ttttcattca
     7681 aatctccttt aaataggttt cattctgttt atattttagc atagaatcag tacaaagggc
     7741 tattttacaa aagaaataaa tggtataata ggaaggcact ttgctgaata gaaaggaaga
     7801 gcagtcaatg attggaatca gtgcatgttt aggcggtctg gcctgtcgct atgatggaca
     7861 gaaaaaagaa gtaccagaat tgatggaact agttgaaact ggagaagctg tcatggtctg
     7921 tccagaagtg atcggcggat taccaattcc tagagatccc gctgaaatca ttggtggaaa
     7981 tggattcgat gtgtggaaag atcaagcaaa agtatggacg acaagcggcg aagatgtaac
     8041 ggaagcgtac aaaaatggcg cgatcaaagc ctatcaaaaa ttgcaggaaa aacacattga
     8101 tctagtcatc atgaaggaaa aaagcccttc atgcggaacc cattcgattt atgacgggag
     8161 tttttccgga ataaaaaaaa atggtgccgg agtggctact gcttatttta ttcagcaaaa
     8221 aatgactgtg ctctctgatg aagaatggct gaaaactaga ggtgagcaga atggaaatcg
     8281 aaaagaccaa tcgaatgaac gcgctctttg aattttattc gaccttattg acagaaaaac
     8341 aaatgaacta catggaatta tattatgcag atgatttttc attaggtgag atcgcggaag
     8401 aatacgaagt aagccgacaa gcagtatatg acaatatcaa gcggacaagc aagatcttgg
     8461 aggattatga aaaaaaactt catctttttt ctaattatat tgttcgagaa caggtattgg
     8521 aaaatatgac tgcatatatt acagataagt acccggaaga taaaaaacta cttgaatatg
     8581 tacagcaaat tcaagcaatc gaagaataaa taaaaggaat gaagaaaata tggcatttga
     8641 aagcttaact gaacgtctcc agcaggcgat gggaaaaata agaaaaaaag gaaaagtatc
     8701 agaagctgac gtaaaagaaa tgatgcggga aatccgcctt gccttactag aagccgatgt
     8761 taacttgcaa gtagtcaaag attttactaa gcgcgtacgc gaacgtgcgg taggggcaga
     8821 agtgctggaa agtttatcac ctgcacaaca aatcgtaaaa atcgttgatg aagaattgac
     8881 gatcacactt ggttctgaaa cagcagagtt aaataaatca ccaaaaatac caactgttat
     8941 catgatggct ggtcttcaag gggcaggtaa aacgaccttt actggtaaat tagccaatta
     9001 tttaaagaaa aatgaaaatg ctcgtccgct cctaatcgca ggggacgtat atcgaccagc
     9061 tgcgatcgac caattgaaag tattaggtca acagctagac gttcctgtat ttgatatggg
     9121 aactgaagtc agcccggtag agatcgtccg tcaagggatg gagttagcaa aagaaaagaa
     9181 aaacgactat gtattgatcg atacggcagg acgtctgcat atcgatgaaa cattgatgga
     9241 tgagttgaaa caaatcaaag aattgacgca accaaatgag attttgcttg ttgtcgatgc
     9301 catgactggt caagatgccg tcaacgtagc tgatagcttc aaccagcaat taggtattac
     9361 aggggtcgtc atcactaaac ttgatgggga tactcgaggc ggggcagcac tatcgatccg
     9421 ctcagtgaca ggcgcaccaa tcaaattcat cggttctggt gaaaaattga ctgacttaga
     9481 agtattccac ccagatcgta tggctagccg tatccttggt atgggggata tgctgacatt
     9541 gattgaaaaa gcgcagcaag actatgacga gaaaaaagcg gaagaacttg cacagaaaat
     9601 gcgggaaaat tcattcgatt tcaatgactt tattgaacag cttgatcaag tgatgggtat
     9661 gggcccactg gaagatctaa tcaaaatgat cccaggaatg aatcaagtcc ctggaatcga
     9721 aaacgtcaag gtagatccaa aagatgttga acgcaaaaaa gcgatggttt attctatgac
     9781 gccagcagaa agagcaaatc ctgatctgtt gaacccaagc cgtcgtcgcc gaattgctgc
     9841 cggttctgga aacagtgttg ttgaagtgaa tcgaatgatc aaacaattca aggaatcaag
     9901 aaaaatgatg cagcaaatgt ccaaagggga tatgaacatc ccaggtatgg accaaatgtt
     9961 tggttcaggc gtcaaaggaa aactaggcaa aatggcgatg aatcgaatga tcaagaaaaa
    10021 taagaagaaa aagaaaaaac ggaaataaga tgaaaaaaac agcttcggta cagactgaaa
    10081 atgtctactg ccggagcttt tctatcagca atttttattc tttactaata caattactct
    10141 aaacgaagag gtggcacaga tgagatgtcc gtggagtgaa caaacagaaa gtatgcggca
    10201 atatcatgat actgtctggg gtgttcctga atatgatgac caaaagctgt tccgaaaatt
    10261 gatgcttgat attaatcaag ccggattgag ctggcagacg attttaaata aaagtgaagc
    10321 attcgatgaa gcatatgatc gattcgatat taaaaaagtc gcaaattatg gagaaacaaa
    10381 aatcagtgaa ctaatggaaa acaaaggaat tattcgtaac cgaagaaaaa tcgaagcagc
    10441 cgtccaaaac gccaaagcag tcttgaaaat tcaagaagaa atcggcagtt tttcagagta
    10501 tctttggtcg ttttctaatg gaaaaatcgt tgattcttct tatcattcac aagaagaagt
    10561 cccaacaaca agtcctcttt cagatgaatt agcaaaagat ctaaagaaaa gaggatttaa
    10621 atttatcgga agcacaacaa tctatgcttt tttagaagct gtcgggatca tcaatgatca
    10681 ccttgacagt tgtttccgca aagaagaggt cgtacgtatc ggcagaaagg gcgaataaag
    10741 catgcgaaaa atcatatttt atggtgctat aagtttagat ggctatttgg caacgaaaac
    10801 ggatgatctt caatggcttt ttgacactcc aacaggtaag caaaccactt atgaagcttt
    10861 ttatgaaaca atcgatacga ccatcatggg cagaaaaact tatcaggaag caaaaaaata
    10921 tttggagacc gaagcaattt atccagagaa aaagaatttc gttttttcca cccacgcaca
    10981 attacaattg ccagatgctg aggtcgtaaa cgaagatcct gttgcttttc taaaaaatct
    11041 acaaatgaca gaaggaaaag atatctgggt tgtgggtggc ggtcgattga tcaaaccgct
    11101 attggaacat caaatgattg atgaatggta tatccagatt acccctgtat tattagggga
    11161 cgggattcct ttattccaaa aaggaacata tgaagcgcgt tttgaaatgc tagacacgac
    11221 acgattcggt gaatttatcg aattgcatta tgtacgaaag tagaagtaaa taaaataaaa
    11281 aaggcaacta tatgcagaaa tgtcctgtac atagttgcct tttttattac cgttatgctt
    11341 tttactctgt taatttgtta gaaaaatcta gatacgcatc taaaatcgtt tggaagaaac
    11401 ttaatgttcc ttcaacgata ttaccatttt catcaattag attcgcaacg tttccgatat
    11461 atgcttccgg ttgctgcaag gttggcacat tcaaaaagac aagtgattgt cgtagatggt
    11521 ggtttgcgcc aaagccgccg atcccacctg gtgatgcagt aacgactaaa cctggtttat
    11581 gatcccaaac actgtgtcca taaggtcttg agcctacatc taaagcattt ttcaatactg
    11641 ctggcacaga acggttgtat tcaggagtaa cgaaaacgac accgtctagt cccctcactt
    11701 cctcacgaaa acgagtccat tcagcaggag cctgttctgg agtttcgaga tcctcattgt
    11761 aaaatggcag atcatcgatt tttacaaata cactttcgta cccttcagga agcaatttcg
    11821 caaaagcttc tgctactttt ttgttgtaag aatcttttct taaactacca ataaaaaatc
    11881 caattttttt tgtcatacga tttttcctcc ttcaaactat ctataacaat cttacaaaaa
    11941 ataagtaact ggagcaaata aaaacactta ctgacaaaag ctacatagaa gatagattac
    12001 ttatttttaa caaaaaagaa gtctaaaatc ttctatttcc taaaaaaaat cgaatttaat
    12061 caagctgtaa agaaaaaaag ctttacaggg taaaaaaaaa ctgttacact acatcttgtg
    12121 aataaaacaa tggaggtgga aataaataat ggcagttaaa atccgtttaa aacgtatggg
    12181 ttctaaaaag agtccgtttt accgtatcgt cgtagctgat tcacgttctc ctcgtgatgg
    12241 acgtttcatc gaaactgtag gtacttacaa tcctttgaaa gaccctgcag aagtagtttt
    12301 aaaagaagac ttagttcttg actggttatc aaaaggtgca caaccttctg acactgtacg
    12361 taacatcctt tcaaaagaag gcgttatgca aaaacaccac gaagctaaat tctcaaagaa
    12421 ataaggtgac cggatatgaa agatttaagc gacttagtct taacaatcgt tcgtccgcta
    12481 gtcacttatc ctgatcaagt caagttagac gtcgtagagt cgaaagactt ttacgagtac
    12541 aatctaacag tggctcctga agatattggc cgtatcattg gaaaacaagg gcgtgttgct
    12601 aaagcaatcc gcacaatcgt ttacggtgtt cggatcaatg caccgaaaaa agtgcgtttg
    12661 aatatcatcg atgataagga aaagtaaata aacgtgctgt actcgaattt tcgagtgcag
    12721 cttttttgtt tttttattaa ttgaagaaat gattatttgg caaaacaagc aagaaagcgt
    12781 atccttatag gaggagggag tgaacagcca atgagaaaga aaatgttatt tattccattt
    12841 gctctattgt taagcgcttg tagttcggtt actcaagaat cagacaatac acatctatca
    12901 agcagtatat ctgaaactgt taccagtcca gaaaaaagta gtaccaaaac ggctaccacc
    12961 actgaacaaa caatggctac ctcaaataat gagaaaaaga ctgctttaga ccagttgaaa
    13021 gaacaacagc caaatgttcc catgcctcta gatgtaccag tctcttcagg ctatctcaat
    13081 atcgcagcga cacatacaaa gcaaggttat tctattttat attacagaac agatcgtccc
    13141 ctcgggttga atgctgacga attgaatcaa gaaacgccta ttgcaaccta tctatatcaa
    13201 tatgggtttg cttctagtca agaaacaatc caagttttgc agccctttga gatcgacaca
    13261 aatggtcagc aagtggactt aggttctcga atcacagggt atcagcaagg tgcagcaggt
    13321 tcaagttttc ttgaatggca agaaggaaac tggcgtattc gaatccgtgg gaataatata
    13381 gaaggccaag atcccttact attagctaaa gaaatcgtag cttatttgga agaaaacagt
    13441 ctaccagcac ctgaacaatt tggtaaaatt actgttgata tgggtgatac gacgaatcga
    13501 gccgtagaag taagctggca agaaccgaaa aatgcctata cgatcactca tcaagatcct
    13561 atgtctgcgt tgaagatggc agtttctatg aagaggttgt gaaatcagcg cgctgatctg
    13621 agcccatcat ttaactccaa gtcacaggaa gtaatcaaaa atagcacgcc gcatttttgc
    13681 ataattttgg cttaatacct taaaagaaaa ctagaattgc ctcttcacac atgctaaaat
    13741 aaaacagaca aaaaagaaat ggggatataa gttgactgaa tacttgaatg taggaaaaat
    13801 cgtaaacaca caaggtatca aaggcgaagt ccgtgttatc tcaacaacgg atttcccaga
    13861 agaacgttac aaaaaaggaa cggtgttaac actattccaa gacggaaaag cacctgtgga
    13921 attaacagtc aaaagtcatc gcaaacataa aaattttgac ttattgagtt ttgaaggaca
    13981 tccttctatc aatgatgtgg aaaaatatcg ggatggcata ttaaaagtgt caaaagacaa
    14041 ctctgtagat ctagctgaaa atgaattcta ttaccatgag atcatcggtc tgcatgtatt
    14101 agaagatgga actgagttag gtaaagtaaa agaaatcttg tcaccaggtg ccaatgatgt
    14161 ttgggtcgtt caacgtccga aaaaagctga tatattgctt ccatatatcg cttctgtcgt
    14221 caaagaagtc gatttagaag ctggagtggt ccgtgtagaa attccagaag gactgattga
    14281 cgatgaagat tgatgtattg actttatttc caagaatgtt tgaaggtcct atgggggaat
    14341 ccatcatcgg taaggctgta gataaaggat tattagatat caatatctct aacttccgcg
    14401 attattcaga taacaaacat cagacagtag atgattatcc atacggcggt ggagcaggta
    14461 tgctgctcaa agtccagccc atctatgata acatccaagc cattgaaaaa gctgcaccgg
    14521 atgtcaaaaa aagagtaatt cttcttgacc cagctggtaa acgattcgat cagaaaatgg
    14581 cagaagaatt ttcacaagaa gaacacttag tattcatttg tggtcattat gagggatatg
    14641 atgaacgtat ccgcacatta gtgacagatg aagtttcctt aggagattat gtccttactg
    14701 ggggagaatt aggtgcgatg gtcatgatcg atgcaacagt tcggcttttg ccagatgtgt
    14761 tagggaatca gacttcagct caaacggatt cttattcaac cggtttattg gaacatcccc
    14821 aatatactcg cccggcagaa tataaaggga tgaaagtacc agaagtattg acaaatggaa
    14881 atcataaatt gattgaagaa tggcaattaa aagaatcact aagaaggacc tatcaacgtc
    14941 ggcctgatct tttggaaaca cttgaatgga cacctcaaat gacgaaattt ttagaagaaa
    15001 tcaagaaaga agaacaagaa aaaacagcag atgaagccaa gtgacttttt ctaaaatatt
    15061 ctttacagta ggttgataat atgttagtat tttatggtga gtgcaaaagc acttaactat
    15121 tacgatattc cgctatgaga aaagctcata agaatatttg gaataaggag aagaaaacca
    15181 tgaatccatt aatcgaagaa ttaacaaaag aacaattacg ttctgacatc ccagcgttcc
    15241 gccctggtga cactgtgcgt gttcacgcaa aagttgttga aggtactcgt gaacgtatcc
    15301 agttatttga aggtgttgtt atcaaacgcc gcggtgctgg aatcagcgaa acttatacag
    15361 tacgtaaagt ttctaacgga gtgggcgttg aacgtacatt cccattgcat acaccacgtg
    15421 ttgcaaaaat cgaagtagtt cgctacggta aagtacgtcg tgcaaaattg tactacctac
    15481 gtgcattaca cggaaaagct gctcgtatca aagaaatccg tcgttaatca ttgttatcag
    15541 actgagaaag aacccttggt attcaagggt tcttttttct tagcatgtaa tttgaacacc
    15601 ctgatccagt ttttttcttt aattattggt aggggagaat aatggattag aataaatcta
    15661 cctataagta gtagtaaaaa aaagagcact atgataatat ctttcttaag agtaaccgct
    15721 ttaataaaaa atccagaata ttctaacata aatggagtat tcttagaatc aaaaggagaa
    15781 agaagatggt ttatacttat acggagcaag aaaaaatgaa agtttgagac acattcatga
    15841 atgatggaaa agtaaaacaa gtaccaagtt caacaaaaaa gaaaaacatc ctattgaaag
    15901 aagtcttgaa acgctttgac catggagtga cttatacaga aacagaggtt aattcgatac
    15961 tgctgaacgt attttctagt ggcgattatg tcgaacaaag acgttatcta atcacatttg
    16021 gctttttcaa aagaagttca gacggtcggg catatcagat gatgggaatt gaaaattaga
    16081 agttttaatt tatactcatt gaaaaaagtt caagtatgcg atattggttc tacaccgcat
    16141 acttgaactt tttttgtcat ctgtatctat aatacaaagc caagaacgtt attcaaaata
    16201 tggatagcta tgataaaagc taatgagcga ttcgcagtta aataagcaat cgtcagtaaa
    16261 atcgttccag gagcgtacat gaaaaaatca attggatgcg ttacatgaat aaagataatt
    16321 ctcttttatt tgtacgaata aataccaaaa cgtacctcct aaaattagat tcaaagaaga
    16381 tagcaaagag gaaaaagata gttctgcagc agctatagcg gcatgtggat tgctagaact
    16441 ttccaaacaa ctgcctgttt cagatcctcg tcatttcgat tacgaagaga tagcaatcaa
    16501 aatcttggaa gaattacaga aaaactatac aacgaaacga gttaacggat caaacggtct
    16561 gcttctccat gccgtttatg ataaaaacag cttaaaaggt gtagatgaat gtgtgatctg
    16621 gggcgattac ttttatgtag aaggaatcac tcgtctagct aaaacatggt actgttactg
    16681 gtgatgtata agagaagagg aggattcata taatgaagcg atggaaaaaa atgatgattg
    16741 gatcaatagt tttaggaatg tcactcgtat ttggtgcttg tggtaatcaa gaagacagta
    16801 gtgaagaatc agcaaatgaa attaccgttt cgacatggaa ttatgaaaca acacctgaat
    16861 tcaaagcgct ctttgaagca ttcgaaaaga aaacgggaat caaagtgaaa gcagtagata
    16921 ttgcttcaga tgattatgat acgaaactca ccacgatgct ttcttctgga gacacgacag
    16981 atgtattgac gatgaaaaat cttttgtcgt attcaaatta tgctttacga gatcagttag
    17041 tcgatcaaac agaacgaatt agagaattag acactgatgc tgctgaagaa acatatgaaa
    17101 tgtatgatat cgatggaaaa acatatgcat tgccatatcg aacggacttt tgggtactat
    17161 attacaataa gaaaatgttt gatgatgcag gaatcgaata tccaaaaaat ctaacttggg
    17221 acgaatatga agatttagca aaaaaactta gtaaaaatga cggacaagtt tacggagcat
    17281 atcaacatat ctggcgttcg acaatccaag ctattgcagc tgctcaaaac gaggcgaatc
    17341 ttgttgaacc agattatcaa tatatgactg attattatga tcgagcattg aggatgcaaa
    17401 aagaaggcgc gcagatggat tttggcacgg ctaaatcaac gaaagtaacc tatcagtctc
    17461 aatttgaaga acaaaaagca gcaatgatgt acatgggaac ttggtatatg gctggcatac
    17521 tcgcaaacaa agaagcaagt aagacagaag tagaatgggg aattacagct attcctcaga
    17581 agaaaaaagg agaatcggtc acgacgtttg gctcaccaac agcatttgct gtcaataaaa
    17641 actcaaagaa acaaaaagcg gcgcaagaat ttattgaatt tgcagccagt gaagaaggag
    17701 caaaagtatt agcaggagta ggagtagttc cttcatatcg tacggatgag attgatcagc
    17761 tttattttaa tctcgctgga atgccaacgg atgaaatttc taaaaaagct ttttctcctg
    17821 atgaaatcaa acttgaattt ccgattgata cgtatgggcc tgcaatcgat aagattttac
    17881 aagaagaata tgacttagtt ttggttggag atgaaacgcc ggaaaaagga acggtaaata
    17941 tggagaaacg tgtagctgcg gaaaaagaat aatcatgaaa ggagagaagc caagagataa
    18001 aacactcttg gtttcgtcat cgaaatggag aatagtataa ataagaaagc tacaaatcat
    18061 ttgcgtagaa aacgaacgtg gatcgcttat tcgtttatcg ccccgaattt cattggtttt
    18121 ttcgttttta cattaatacc aatcctgttt tcgcttgtac tagcatttat gaaatgggat
    18181 tctttttcaa cgccagaatt tgctggacta gataactttc gtaaaatggc caacgatgag
    18241 acattttgga tttctttgaa aaatactttt ttatataccg ctgctgtagt gcctggcaca
    18301 cttgtttgtt cgttagggct agctttacta ctaaatcaga aaattagagg gatgaagctt
    18361 tttcgtacga tatttttctt tccgtatgtt acttccttag tagcaattgc tgttgtttgg
    18421 aatatgttgt ttcatccaac agtgggacct attaatatct ttttaagacg atttattgaa
    18481 catcctccag gttggacttc aagttcaaat tgggcgcttt gggcaattgt aattgtcagt
    18541 atttggcgag ggatgggtta ttacatgatt ctataccttg caggactaca aagtatccaa
    18601 aaagaactat atgaagcgtc ttcgctagat ggagcaaacc gttggaaaca atttttgaat
    18661 gtgacattac cttcgttgag accaacaacc ttctttgtaa cgatcatgct tgtcatcaat
    18721 tgtttcaagg tgtttgacct tgtgcaagtg atgacggatg gcggccctgg tcgagcgaca
    18781 aatgtgctgg tttaccaaat ctacaatgaa gcattcaaaa aatttgattt tggttatgct
    18841 tcagctattt cacttgtttt gttcgtaatt gttttgggcg ttacggttgt tcaattttct
    18901 tggaataagc gtcgtactta ggaggtgtga tatggaagag agtgttcgaa atgaaagaaa
    18961 aaaagcaaaa attcgttcaa aaaacatgcc ctttattcaa tggttgggta tgattctgct
    19021 gattgtatta gctttattga cgatcattcc ttttttatgg atggtttctg cttcattcaa
    19081 agcaaataat gaagtgttaa cgattccgat tcaatggatc cctaaaactt ggcatttaga
    19141 gaattatctg gttatttggt ctcgtattcc tttagtgaca ttttttaaga atacagcttt
    19201 tttaagcgtt gtgatcacgt tgattcagtt gtttacttct agttttgctg tttacgggtt
    19261 ttctgaacta aattttaaag ggcgcgatat tttattttta gcatatatcg gcacgattgc
    19321 tgtcccttgg cagtcttata tgatcccgca gttcattatg atgagaaaag tgggtttaac
    19381 agatacgttg tggtcgttgg tgctattaca agcgttcaat gcttttggtg tgttcttgtt
    19441 gaagcaatat tacagtagta tcccagattc tttatgtgag agtgcgcgga tcgatggctt
    19501 atcagagtgg gaaatctatc gcaagatcat tttacctctt acaaaaccgg cgattgccag
    19561 tttgacgatc atcacattcg ttaatacatg gaacgattat atggggccat tcatttattt
    19621 gtcttctacg gaaaataaaa caatccagtt agggttgaaa atgtttattg ggctatttga
    19681 tgcagagtac gcattaatca tggctgcctc agttgtttct gttcttcctg ttgcttttat
    19741 atttttagcg atgcaaaaat attttgtaga agggatcgct tcatcaggaa tgaaggaata
    19801 ggaggtgatt atgaaagagc agcgttttga caaaaatgtt tatatggaat tcttttattg
    19861 gttgtatcaa tcattggcaa gtagcctttg tttttggtgt gtcaatatct tgtatatagc
    19921 agctgcgttc cttttgtcga tagatggaag gaatgtcttt tcctttattt tttctttact
    19981 ttttattggt ccaggttgca tcgtgatgat aggtatgaca gaagaattag caaaagaaaa
    20041 aagtttttca ccagtacgtt tgttcataaa gacgtttcgc caattttggc taaagggatt
    20101 tttttattgg ctatttgctt ggatagttag tgtgataatg atttttgatt gtttcttttt
    20161 tatacgtttt tcttatggaa aatggttgat tccgttgttt gttttacttg cttgttttac
    20221 ttgcttgttt atcagtttcc tttagcatca actgctggta ttttcaggtg agaaatccag
    20281 cgtcgaaacc aaatcaagtt ctacggatag cgttctacta tacgttgaaa aaatggtatg
    20341 tgagtttact agatttttta ctattgacaa gtttgtttct cttctttttc gtaaagcccc
    20401 aatggtgtat tctactaggt ccttctatcg tatttggttt gatttacttc aacaatcgta
    20461 aattgatgag aactatggat ctgtagactt tataaattga aactttcaat taagaatgac
    20521 agttttttat gtaaaaactg tcattctttt tca
//
LOCUS       DAZKLD010000042        19679 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.042, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000042 DAZKLD010000000
VERSION     DAZKLD010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19679)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19679)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19679
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.042"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            7..330
                     /locus_tag="ACG8KR_001231"
     CDS             7..330
                     /locus_tag="ACG8KR_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HFQ0200611.1"
                     /translation="MFESFDSSRNRYASVGVVSSLPDELIDSIWFIIDLDLKGVIPLE
                     NILTFDLIENRGKVTMHFSQAGSNVEMDIDLPFAYSNQFPAQVYAYDDGTRETILLPS
                     EIRQY"
     gene            512..1882
                     /locus_tag="ACG8KR_001232"
     CDS             512..1882
                     /locus_tag="ACG8KR_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HFQ0200612.1"
                     /translation="MRDLTKGSPAKLILMFTVPLLIGNVFQQFYNMIDMIIVGQTLGK
                     NALAAVGATGSLTFLIIGFAQGLTAGLAIITAQRYGAKDYRGLKKSFAASVVISLVVT
                     VILTVLSLLFIRPMLQLMQTPPEILDQAQTFISIILLGIFASVSFNLLSNVIRALGDS
                     RTPLFFLIIAVIINVVLDLIFIIYFGMGVEGAAIATVIAQVSSSVLCLVYIKKKMPLL
                     QLRKKDFKFEKEEFAVHLNAALPMAFQSSIIAVGAIVLQAALNSLGTDVVAAQAAAGR
                     IDQFANQPMMSFGIAMATFTAQNYGAKEYGRILQGVRQTLLMSVGFSLLAGAIVIFFG
                     HFFVQLFVSPSETRVFELAQTYFNINGSLYWILAVLFILRYTLQGLGQSKIPTIAGMM
                     ELLMRSFAAIVLTGMWGYPGAAAASPLAWIGSVAVLLYSYMRSMKQLKHMGEEEHFTL
                     EHAEAR"
     gene            complement(2012..3313)
                     /locus_tag="ACG8KR_001233"
     CDS             complement(2012..3313)
                     /locus_tag="ACG8KR_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HFQ0200613.1"
                     /translation="MVINFIILFLMIAITSFFVASEFALVKIRRSRLEQLEKENVKNA
                     KLALHVTHHLDNYLSASQLGITLTGLIIGWVGEGSVAALLEPVIGKLPFSSAISSTIS
                     VALGFILVTYIDVVIGELLPKSYSIVNTEKVVLFVVKPLHYFYKVMFPFIWVLNHSAA
                     GLGKLLGVRLVSEGEETLSQEELTLVALNSYEKGELTKEEYYYLENVFEFDDTLAKDI
                     QVDRTSMQVFEASETVKEAIKASIEQGHTRYPVILESKDNVLGYVTLPGLIKQSYKDD
                     QLTVEQLIEEPIVTTETIPIKKLLTIMRKKGKHIAILKDEYGGTSGLVTIEDILEEIV
                     GEIRDETDLDEALIAEQSDGSYIISGKLTLDDFQRYFHVEIPEFEETNFTTLAGFASS
                     RYKEIKAGTIIEIASFRFTVLEYQHAHIDYFKVESTERKTE"
     gene            complement(3667..5658)
                     /locus_tag="ACG8KR_001234"
     CDS             complement(3667..5658)
                     /locus_tag="ACG8KR_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HFQ0200614.1"
                     /translation="MATTHNTKKISMTGLLVAMGVVYGDIGTSPLYVMKSIVEDNGGL
                     SGINPDFVIGSVSLIFWTLTLLTTIKYVVIALNADNHGEGGIFSLYTLVRKGGKYLII
                     PAMIGGAALLADGVLTPAVTVTTAIEGLRGIPQFFDRFGNNQNVIVIITLVIIFVLFM
                     VQRFGTDIVGKAFGPIMFAWFTFLGVMGLMNFSQDWTVIRALNPYYAIQLLFSADNKL
                     GLFILGNIFLATTGAEALYSDLGHVGKRNIRLSWPYVKICLVINYFGQAAWLLNVYQN
                     PEAQQIKNLNPFFQMMPQSWIVIGVAFATVAAVIASQALITGSFTLVSEAIKLKLLPR
                     LKIMYPGNSIGQMYIPAVNLILWIACSLVVVTFRTSHHMEAAYGLSITVTMLMTTALL
                     YFYLIQNGHSRWLAYLVTFFFGAIEVVFFISSVVKFFRGGFVAVLIGLIILVVMFIWE
                     QGNIIRESSTEDVSLRDYIPQLRELRDDTSLPIYQTNVVFLVPDMAEDKVGRQFIYSI
                     LDKRPKRARVYWFVHVEVTDEPYTKEYQVDMMDTDFIVQVNLFLGFRVQQEINVYVRQ
                     VIHDLMKQGRLPKQPQRYSLTPGREVGDFQFVLIDEVVSNVTTLGKWQRQIMQAKLAI
                     KKIATSPETWFGLEYSEVKHETVPLIIGNTRKTWLKERL"
     gene            complement(5802..6068)
                     /locus_tag="ACG8KR_001235"
     CDS             complement(5802..6068)
                     /locus_tag="ACG8KR_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFQ0200615.1"
                     /translation="MLNFLWSLFIGGILGLIAGAILGKEVPGGVSGNIIIGFLGSWLG
                     EFLLGPLGPELGGFYLIPALLGAIFCLALYSFVARRIRERPKKE"
     gene            complement(6578..8704)
                     /gene="pnp"
                     /locus_tag="ACG8KR_001236"
     CDS             complement(6578..8704)
                     /gene="pnp"
                     /locus_tag="ACG8KR_001236"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HFQ0200616.1"
                     /translation="MSEKQVFKTTWGGRPLQIEVGQLAKQANGAVLVRYGDTVVLSAA
                     VASKEAKDTDFFPLTINYEEKMYAAGKIPGGFIKREGRPSTEATLTARLIDRPIRPMF
                     AEGFRNEVQVTNIVMSVETDCSPAMAAMLGSSLALSISDIPFDGPIAGVEVGRVNGEY
                     VLNPTVEQAEQTDIELTVAGTKQAINMVESGAKEVSEEDMLGALLFGFDAIKELVAFQ
                     EEIVQAVGKEKMEVTLLQVDEVLKKEIFDASYATMKAAVMTEEKLAREDNIEQVKIDI
                     REAYAEKFAGHEDEDHLLKEVKQITEDLEKDVVRELITIDKIRPDGRKLDEIRPLSSE
                     VSLLPRVHGSGLFTRGQTQALSACTLAPLGEHQIIDGLGVEVSKRFIHHYNFPQFSVG
                     STGRAGSPGRREIGHGALGERALAQVIPSEEEFPYTIRLVAEVLESNGSSSQASICAG
                     TLALMDAGVPIKAPVAGIAMGLVSDGENYTILTDIQGLEDHLGDMDFKVAGTKDGITA
                     LQMDIKIQGITEQILTEALTQAKQARMEILEELTSTIAAPREELSQYAPKIEMIQIEP
                     AKIKDVIGKGGDTINGIIDETGVKIDIDQDGKVSIASADAEMIKKAIKIIEDLTKEVK
                     VGEVYLGKVVRIEKFGAFVNLIKGKDGLVHISQLANDRVNKVEDVVKLGDEILVKVTE
                     IDKQGRVNLSRKAMLNEDGSTKNEEK"
     gene            complement(8892..9161)
                     /gene="rpsO"
                     /locus_tag="ACG8KR_001237"
     CDS             complement(8892..9161)
                     /gene="rpsO"
                     /locus_tag="ACG8KR_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HFQ0200617.1"
                     /translation="MAISKERKNEIINEYARHEGDTGSPEVQIAVLTEEINHLNEHAR
                     VHKKDHHSYRGLMKKVGHRRNLLAYLRKTDVQRYRELIKRLGLRR"
     gene            9412..9975
                     /gene="def"
                     /locus_tag="ACG8KR_001238"
     CDS             9412..9975
                     /gene="def"
                     /locus_tag="ACG8KR_001238"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFQ0200618.1"
                     /translation="MITMDDIIREGNPTLREVAKEVSLPLSEEDISLGKEMLEFLKNS
                     QDPIKAEELHLRGGVGLAAPQLDISKRIIAVHVPSPDPEADGPSLSTVMYNPKILSHS
                     VQDACLGEGEGCLSVDREVPGYVVRHAKITVSYYDMNGEKHKIRLKNYESIVVQHEID
                     HINGVMFYDHINDQNPFALKEGVLVIE"
     gene            complement(10228..10749)
                     /locus_tag="ACG8KR_001239"
     CDS             complement(10228..10749)
                     /locus_tag="ACG8KR_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HFQ0200619.1"
                     /translation="MIEGETRRKQIMQTLANTEKPVSASKFAKTFGVSRQIIVGDIAL
                     LRAAGKSIVATARGYILETEETNTGHISKIAVQHGKDQTEEELRLIVENGGEIIDVIV
                     EHPLYGELAGTLHIKTPQDIDSFMRRYQKSNATLLSELTGGIHLHTIRYPEKHMLKNI
                     KQKLAEAGILYEG"
     gene            complement(10752..11594)
                     /locus_tag="ACG8KR_001240"
     CDS             complement(10752..11594)
                     /locus_tag="ACG8KR_001240"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HFQ0200620.1"
                     /translation="MTEFKTEKEKMIAGELYFANDPELVADRMFARSQSQIINQAESA
                     ELRSQLLKETFGRTGKKIYMEPVINFDYGYNIFVGENFYANFNCTFLDVSTIEIGDNC
                     MFAPNVQLYTATHPLHPVKRNSGLEYAKPIKIGNNVWLGGGVIVTPGVTLGDNVVVGA
                     GSVVTKSFPDNVVIAGNPARIIKTVEEELTEESLETLRHKIDMIDRELIALLEKRMDT
                     VTKIGQVKKEENQAVYDAKREQQVLDKVVSLLKNKGYKETITDTYVDLMKHSREYQNK
                     MKEE"
     gene            complement(11587..12312)
                     /locus_tag="ACG8KR_001241"
     CDS             complement(11587..12312)
                     /locus_tag="ACG8KR_001241"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0034979 - NAD-dependent protein lysine
                     deacetylase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HFQ0200621.1"
                     /translation="MKQLTVDEAAKEILAYQQITFLTGAGVSTPSGVPDYRSLSGIYH
                     GMEQPEYLLSHQAMVNEPDKFYQFVKKIYHPEAKPNIIHLEMAHLAKEKNVWVVSQNI
                     DGLHAKAKSPQLVNFHGSLYQCHCRKCQQTVDWKEYLHSDKHQACGGQIRPDIVLYGE
                     GFQDGVMEQAAYAVSQAELIVIVGTSFQVHPFCDLVQFRQPSAKILVINQTPVYLAEP
                     YTFIQEDGAVVFKKIQELGADYD"
     gene            12488..12784
                     /locus_tag="ACG8KR_001242"
     CDS             12488..12784
                     /locus_tag="ACG8KR_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0200622.1"
                     /translation="MLPFFIYWGVILACIAWLALSVYFSVFYLVRKENGNLWAFALFN
                     VIAAIVLAITLAVYRTWGWGITQYSSLIYLILAIYGVVVLLQAILGREPKKAAA"
     regulatory      13040..13214
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00168"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Lysine riboswitch is most abundant in Firmicutes
                     and Gammaproteobacteria where they are found upstream of a
                     number of genes involved in lysine biosynthesis, transport
                     and catabolism; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="lysine"
                     /db_xref="RFAM:RF00168"
     gene            13352..14944
                     /locus_tag="ACG8KR_001243"
     CDS             13352..14944
                     /locus_tag="ACG8KR_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327538.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFQ0200623.1"
                     /translation="MKKNMVSVAVLFAFVFSLFSVGSLANADEKTPSDEFRVGMEAGY
                     APFNWSQQTDENNALPIQGQNSYAGGYDVQIAKKVADGLGKKLVIVQTKWDGLAPALQ
                     SGKIDAIIAGMSPTAERRKEIDFTDPYYESQLVVVVQKDSKYAKAKSLADLSGAKITA
                     QLNTFHYTVIDQIPDVQKQQAMDNFSAMRTALASGMIDGYVSERPEGVTATSVNKDLE
                     MLEFSGADGFQTNPEDVQISVGMRKGDPDIQKVNQILSGISNDERTKIMDQAVKDQPA
                     ATDSEEKPGIISDFTTIWKQYGNMFLRGAGLTLFIALIGTVVGTTLGLLIGVFRSVPE
                     SGNALTRFFQKIGNALLSIYVEVFRGTPMMVQAMVIFYGLALAFGISLNRTIAALFIV
                     SLNTGAYMTEIVRGGIFAVDEGQFEAAQAIGMTHGQTMRKVVIPQVLRNILPATGNEF
                     VINIKDTAVLSVIGVADLFFQGNSASGANFQFFQTFTIVGIIYLVMTFTITRILRAVE
                     KKIDGPSAYTKLEEIDNSNLQE"
     gene            14957..15697
                     /locus_tag="ACG8KR_001244"
     CDS             14957..15697
                     /locus_tag="ACG8KR_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFQ0200624.1"
                     /translation="MNEKIIEIKHLRKSFGENEVLKDISVTVNKGEVVTIIGSSGSGK
                     STFLRCINLLEKPTGGEIIYNGENVLAPKYNLPKYRTNLGMVFQSFNLFNNMNVLENC
                     MSGQTTVLKRDKETAKKVAIENLEKVGMDRYIEAKPAQLSGGQKQRVAIARALSMNPD
                     VLLFDEPTSALDPEMVGEVLNTIKDLAHTGLTMLIVTHEMEFAKDVSDRVIFMDKGVI
                     AEEGTPEDIFVHPKEERTKEFLSRILNA"
     gene            15865..17214
                     /locus_tag="ACG8KR_001245"
     CDS             15865..17214
                     /locus_tag="ACG8KR_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFQ0200625.1"
                     /translation="MNEEQELQRGLKNRHVQLISIGGAIGTGLFLGSGKSIQLAGPSI
                     LLAYLITGCICFFIMRALGELLLSNTNNHSFLDFVAEYLGKKAAFITGWTYWFCWVSI
                     AMADLTAIGMYVRYWAPGVPQWLPELIALGLLLCLNMVAVSLFGELEFWFALIKVVAI
                     IAFILVGAYMILTHYTTDIGTASITNLWSHGGFFPTGAKGFILAFQMVTFAFAGIELV
                     GLVAGETENPEKVLPEAINNIPIRIILFYLGSLFVIMAIYPWNSLNPDNSPFVEVFSE
                     IGITIAASLINLVVLSAAASACNSAIYSTGRMLRSLAQEGSAPKKFKKLTTHHVPGNA
                     LTFSTIVIFISVILNYVMPSEVFTLVSSIATTCFLFIWSMLVYTHMKYRKSILGKKAH
                     SFKMPLYPFSNYLVFLYMAFVCVVLFLGKDTRIALLLTPVWFLLLLLIYHMKYERKK"
     gene            complement(17342..17932)
                     /locus_tag="ACG8KR_001246"
     CDS             complement(17342..17932)
                     /locus_tag="ACG8KR_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HFQ0200626.1"
                     /translation="MTKSRLEAFTDGVVAIVLRVLVLEIKIPNTSSFHSLWVIRNTLL
                     AYTISFIFVAVIWVNHHRIFQMAERINYRVVWSNIFLLFWLTLCPSVTSWVGRNPEAF
                     WPEMAYVLVYTMWSFSFGILMRQIMKANKPDSHVVRVLSKDRRSLISMMINLCLIAGV
                     FFVPLIGLFGRFFVSGIWIFSYKKADAYYQRLFPGK"
     gene            18079..19431
                     /gene="brnQ"
                     /locus_tag="ACG8KR_001247"
     CDS             18079..19431
                     /gene="brnQ"
                     /locus_tag="ACG8KR_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HFQ0200627.1"
                     /translation="MTKKLSFKDYLFIGSMLFGLFFGAGNLIFPVHLGQEAGAATFWA
                     NLGFLVTGIGLPFLGVIAIGVSKSSGVYELAKRINQTYAMVFTILLYLVIGPFFALPR
                     LATTSFEIGLAPFIDPSRQTLYLAIFSIVFFVLAWWFSRKPTKILDYVGKFLNPAFLV
                     LLGALLLLAFLNPLGSVNQAPIQPNYQEHAFFTGFTQGYNTLDALAALAFGIIIVTTI
                     QNMGVTKPAEIAKDTIKSGAISIILMGIIYTLLAYLGAMSLGSFALSENGGITLAQIA
                     DHYLGTYGSILLAFIVILACLKTGIGLITAFAETFTDLFPQRNYAFFVALASILPCLA
                     ANVGLTNIIQFSLPVLMFIYPLAMTLILLVLVGPLFKQRTAVYRMTTYFTLIASIFDG
                     LNACPETIKQTPIVQNLLYVAESYLPFFKLGMGWIVPAVIGFVIGLIWSFAKKEEVAD
                     "
BASE COUNT         5619 a         3969 c         3805 g         6286 t
ORIGIN      
        1 aaacagatgt ttgaaagttt tgatagcagc cgcaaccgtt acgcttcagt tggtgtcgta
       61 tcaagtttgc cagatgaatt gattgacagt atatggttta ttattgacct agatcttaag
      121 ggagtcattc cattagaaaa tatattaacc tttgatttga tcgaaaatcg tggaaaagtg
      181 acgatgcatt tttctcaagc aggaagcaat gtggaaatgg atattgattt gccgtttgcc
      241 tattcgaacc aatttcctgc tcaagtatac gcctatgatg acggtacgag agaaacgatc
      301 cttttaccaa gtgagattag acaatattaa ggtgatagat aaacgtggat gtcccttatt
      361 ttacagacca ggccaagttg ttgattcttt gactcaattc ttggcttctt tttatggagt
      421 ttcataaatt atcattgatt tctaattggt attctcagga taaacgctat atactactaa
      481 ctaaatttta aagatataag gagtttaaaa gatgcgcgat ttaacaaaag gaagccccgc
      541 taaactgatt ttgatgttca ctgttccttt actgatcgga aatgtttttc agcagttcta
      601 caatatgatc gatatgatca tcgtgggtca gacgctaggt aaaaatgcgc tggcagcggt
      661 tggtgcaact ggaagcctga catttttgat cattggcttt gcccaaggtt taacagctgg
      721 tttagccatt attaccgcac aacgttacgg tgcaaaagat tatcggggtc tgaagaaaag
      781 ttttgctgct agtgttgtca ttagtcttgt cgtcactgtg attttaactg tcctaagtct
      841 cctgtttatt cgtccgatgc ttcaattgat gcaaactcct cctgaaatac ttgatcaagc
      901 acaaacattt atttcaatta ttttattagg gatctttgca tcagtcagtt tcaatttgct
      961 ttctaatgtc attcgcgcac ttggtgacag ccgcacacca ctattctttc tgatcattgc
     1021 ggtcatcatc aacgtcgtac ttgatttgat ctttattatc tattttggca tgggtgtgga
     1081 aggtgcagct attgctacag taatcgcaca agtttcttcc agcgtactgt gtctagtgta
     1141 tatcaagaaa aaaatgcctt tgttacagtt acgcaaaaaa gatttcaaat ttgaaaaaga
     1201 agagtttgct gtacacctaa atgctgcttt gccaatggcg tttcaatctt cgatcatcgc
     1261 tgttggtgct atcgtgttac aagcagcact aaatagtcta gggacagatg tcgtagctgc
     1321 tcaagccgct gctggtcgta tcgaccaatt tgcaaatcag ccgatgatgt catttggtat
     1381 tgcaatggca actttcactg cacaaaacta tggtgcgaaa gaatatggcc gaatcttgca
     1441 aggggtacgt caaactttac tgatgagtgt cggatttagt ctcttagcag gtgctatcgt
     1501 cattttcttc ggtcatttct ttgttcaatt gtttgttagc ccatcagaaa cacgtgtatt
     1561 tgaattagca caaacctact tcaatatcaa tgggtctctt tactggatct tagcagtcct
     1621 gttcatttta cgctacacac ttcaagggct tggacaaagc aagatcccta cgatcgccgg
     1681 tatgatggaa ttgcttatgc gttcttttgc agctatcgtt cttacaggaa tgtggggata
     1741 cccaggagca gcagcagctt ctcctttagc ttggatcggt tctgttgccg tgttgcttta
     1801 ctcttatatg cgttcgatga aacaattgaa acacatgggt gaagaggaac atttcacatt
     1861 agaacatgca gaagcaagat agccttgatt aaaagaaatc gttgcggcta aaaatgaaaa
     1921 cttttattga aaatatcttc ttcgtttacc gaaaacaggt tttttagata acaaaaaggc
     1981 tgtgacaaaa aatgtcacag ccttttttct tttattctgt ttttctttcc gtggactcta
     2041 ctttgaaata atcgatatgt gcatgctggt attctaatac cgtgaaacgg aatgatgcaa
     2101 tctcaatgat tgttcctgct tttatttctt tatatcggct agaagcaaag cccgcaagtg
     2161 tagtaaaatt cgtttcttca aactctggaa tctctacatg aaaataacgc tggaaatcat
     2221 ctaatgttag tttgccggag atgatataag agccatctga ctgttccgct atcaatgctt
     2281 cgtccaaatc tgtctcatct cggatttccc cgacgatttc ttctaaaata tcttcaatcg
     2341 tcactaagcc gcttgtccct ccgtattcat cttttaagat agcaatatgc ttaccctttt
     2401 ttcgcatgat cgtcaataat ttttttatag ggatcgtttc agttgttacg atcggctctt
     2461 caatcaactg ttctactgtc agctggtcat ctttataaga ttgcttgatc aaacctggca
     2521 aggtgacata acctaaaaca ttgtcttttg attctaagat aaccggatag cgagtatgtc
     2581 cttgttcaat cgaagctttt atcgcttctt tgacggtttc tgaagcttcg aatacttgca
     2641 tggacgtacg gtctacttgt atatcttttg ccaaggtatc atcaaattca aacacgtttt
     2701 ccaaataata atattcttct ttggtcaact ctcctttttc ataagaattc aatgcgacta
     2761 atgtcagctc ttcttgagac aatgtctctt ctccttcaga tacgagacgc acacctaata
     2821 gcttccctag ccctgcagct gaatgattca atacccaaat aaaaggaaac attactttat
     2881 aaaaataatg aagtggttta actacgaaca agacgacctt ctccgtattg acgatactat
     2941 agctcttagg aagcagttcg ccaataacca catcgatata cgtaactaaa ataaatccca
     3001 gcgccacaga gattgtactg ctaatagccg aactaaaagg tagtttaccg atcactggtt
     3061 ctagtaaagc cgcgacagat ccttcaccga cccacccgat gatcaaaccg gttagcgtaa
     3121 ttcctaattg actagctgat aaatagttat ctaaatggtg tgtaacatga agagctagct
     3181 tggcgttctt tacattttcc ttttctagtt gttccaatct tgatcgtctg attttcacta
     3241 aagcaaattc acttgctaca aaaaatgaag taattgcaat catcaaaaat aaaataatga
     3301 aattgataac catattctcc cgcctttctc tttttctatt gtaccctttt tctacaaaaa
     3361 gattgaaaat aatgcgtatt catatttagg aataaaaaag gagatgcggc atttttcagc
     3421 cacatctcct tatcacttca aaatcattgc gttacttgtt gctaaacagc tttttcatga
     3481 cctctgataa aaacggtatt caaaagcttg catctacggc attaagccca aacagatgaa
     3541 aaacatgaga agctgttttt atctgctcgt tcttactgct tgttgcagta cagctttttc
     3601 atgacctctg ataaacggtg ttcaagtagc tgacattcga cattagttca ggtcaaagcg
     3661 ctactttcac aaccgctctt tcaaccatgt tttccgagta tttccaatga taagcggaac
     3721 agtttcatgt tttacttcgc tgtactctag cccaaaccat gtttctggcg aggtagcgat
     3781 ttttttgatt gccagttttg cctgcatgat ttgacgttgc cattttccta gagttgtcac
     3841 attggaaact acttcatcaa tcaggacaaa ttggaaatcg ccaacttctc gccctggtgt
     3901 cagagagtat ctttgtggct gttttggcaa acggccttgt ttcatcaaat catggatgac
     3961 ttggcgaaca tatacgttga tttcctgttg gaccctgaaa cctaagaaca gattcacttg
     4021 gacaataaaa tcagtgtcca tcatatctac ttgatattct ttcgtatatg gctcatccgt
     4081 cacttccaca tgaacaaacc aatagactcg tgctctttta ggccgtttat ctaaaatcga
     4141 atagatgaac tgtcggccaa ctttatcttc agccatatca ggaaccaaga agaccacatt
     4201 tgtctgatag attggtaaag aagtatcatc acgtaattct ctaagctgcg ggatataatc
     4261 tctcaaagac acatcctcgg ttgacgattc gcgaataatg ttcccttgtt cccaaataaa
     4321 catcacgact aagatgatca aaccaatcag aacggctaca aaaccgcctc ggaagaattt
     4381 gacaacactt gagataaaga aaacgacttc tatcgcaccg aagaaaaagg taactaaata
     4441 tgcaagccaa cgagaatgcc cattttggat aagataaaag taaagcagtg ctgttgtcat
     4501 caacatcgtc acagtgatcg atagaccgta agcagcttcc atatgatgag acgttcggaa
     4561 tgtgacaacc actagagaac aggcgatcca taaaatcaga ttgactgctg ggatatacat
     4621 ttgcccgata ctgttcccag gatacatgat ctttaaccga ggcagcagtt tcaacttgat
     4681 tgcttctgaa actagcgtaa acgaacctgt aatcaatgct tgcgaagcaa tgactgcagc
     4741 gactgttgca aaagcaacgc cgatcacgat ccaactttga ggcatcattt ggaagaatgg
     4801 attcaggttc ttgatttgct gtgcttcagg gttttgataa acattcaaca accacgcagc
     4861 ctgcccaaaa taattgataa ccaaacaaat tttgacgtaa ggccagctca gacgaatatt
     4921 tcgtttgccg acatgtccaa gatcggaata aagcgcctct gcacctgtcg tagccaggaa
     4981 aatatttcca agaatgaaca agcctaattt gttatctgca ctaaaaagca gctggatagc
     5041 ataataagga ttcagtgcac ggataactgt ccagtcttga ctgaagttca ttaatcccat
     5101 tacgcctaaa aaagtaaacc aagcgaacat gattggtccg aatgcttttc cgacaatatc
     5161 tgtaccaaag cgttggacca tgaataaaac aaaaataata actaaagtga taatgacgat
     5221 cacgttctgg ttattaccga accggtcaaa aaactgcgga attcctcgca agccttcaat
     5281 cgcagtcgtt accgtcacag caggggtcaa taccccatct gctagtaagg cagctcctcc
     5341 gatcattgca ggaatgatca aatatttccc accttttcgg acaagtgtgt agagtgagaa
     5401 aattccacct tcaccatggt tatctgcatt caatgcaatc acgacatatt tgattgttgt
     5461 cagtagagtc aatgtccaaa aaatcaatga gactgatccg ataacaaaat caggattgat
     5521 tcctgacaat ccgccattat cttcaacaat cgatttcata acgtaaagcg gacttgtccc
     5581 gatgtctccg tagacgactc ccatcgctac aagcaggcca gtcattgata tctttttggt
     5641 attgtgcgtt gttgccacgt ttttccctca tttcttgtta tatggatatc ctttaggtaa
     5701 gcttgaatga tagatgatat tcttatccat gacacccgct ttcctaaatt gcttctaatt
     5761 atccaagtaa cacactgaag tgtcaactta tttgaataaa attattcttt ctttggacgt
     5821 tctctgatac gtcttgccac aaatgaataa agagcaaggc aaaaaattgc acccaataat
     5881 gctggtatca aataaaaacc gccgagttcc ggtcccagtg gtcctagtaa aaattcgccg
     5941 agccaactac ctaaaaaacc gataatgata tttccagata cgcctcccgg gacttccttt
     6001 cctaaaattg cgccagctat caaacctaat ataccgccga taaataacga ccataaaaaa
     6061 ttcagcatct cctttccctc ctttatccat atttctcact tttattttac gctttattca
     6121 gaaggactcc aaaaatatgc gccaagaaac tccagaaaaa aagaagttgt aacaaaagtg
     6181 ttcagcttat ttccgtagaa gctgcttctg gaacaccgtg aatcagaggt tgtgaaatca
     6241 gcggtttgac ctgagtatta gagaaacaaa cggaaaaata gttcctcata ttttgccgtt
     6301 ttttgagcta atatcgaggg acagctgatt gaacatcgtt tattggagag gttgtaaaaa
     6361 agctgttctg catcaagtaa taagaacagc caaataaaaa cagctcctaa tgtttttaac
     6421 ttggctggac ttattaccgc agatgcaagc ttttgaacac cgtttattcg aaaaaaaagg
     6481 actgtgacac ggcttttgtc acagtcccct tttttcgaat aattattgtc cccctgtcac
     6541 cttcacgatc acaagtacat tattcctatg aattaaatta tttttcttca tttttcgttg
     6601 aaccatcttc gttcaacatt gcttttcttg acagatttac gcgtccttgt ttatcgattt
     6661 cagttacttt cacaaggatc tcatcaccta gtttaacaac atcttcgact ttattgacac
     6721 gatcgtttgc taattgggaa atatggacaa gtccatcttt tcctttgatc aagttgacga
     6781 atgcaccgaa tttttcgata cggacaactt tccctagata aacttcacca actttgactt
     6841 ctttggtcaa atcttcgatg atcttgatag cttttttgat catttctgca tcagctgaag
     6901 caatacttac ttttccatct tgatcgatat cgattttcac accagtttca tcgatgattc
     6961 cgttgatcgt atcgccgcct ttaccgataa catctttgat tttagcaggt tcgatctgga
     7021 tcatttcgat ttttggtgca tattggctta attcttcacg tggagcagca atcgttgaag
     7081 tcaattcttc taagatttcc atacgtgctt gttttgcttg agttaatgct tcagttagga
     7141 tttgttctgt gattccttga atcttgatat ccatctgtaa agcagtgata ccatcttttg
     7201 taccggcaac tttaaagtcc atatcgccaa gatgatcttc cagcccttgg atatcagtta
     7261 ggatcgtata gttttcacca tcagaaacaa gtcccatcgc aataccggcg actggcgctt
     7321 tgattggtac acctgcatcc atcaatgcta atgtgccagc acagatactt gcttgggaag
     7381 aagaaccatt tgattccaat acttctgcta ctaaacggat cgtataagga aattcttctt
     7441 ctgaagggat tacttgtgct aacgcacgtt cacctaatgc gccatgtccg atttcccgac
     7501 gccctggaga gcctgcacgt cctgtagaac caacagaaaa ttgagggaaa ttgtagtggt
     7561 ggatgaatcg tttgctgact tccaccccta aaccatcaat gatttggtgc tctcctagag
     7621 gtgcgagtgt acaagctgat aaagcttgtg tttgtcctcg tgtgaacaaa cctgatccat
     7681 gtacacgtgg caataagctg acctctgatg acaatggacg gatttcatcc aatttacgcc
     7741 cgtcaggacg gatcttatcg atggtgatca attcacgtac gacatctttt tcaagatctt
     7801 ctgtgatttg tttaacttct ttcaacaagt gatcttcatc ttcatgacct gcaaatttct
     7861 cagcataagc ttcgcggata tcgatcttca cctgctcgat attatcttcg cgagcaagtt
     7921 tttcttcagt cattactgct gctttcattg tagcgtaaga agcatcaaag atttcttttt
     7981 tcaatacttc atcaacttgc agtaaagtca cttccatttt ttctttccct actgcttgaa
     8041 cgatttcttc ttggaaagca actaactctt tgatagcgtc aaaaccaaac agcaatgcac
     8101 caagcatgtc ttcttctgaa acttctttcg cgccgctttc aaccatattg atggcttgtt
     8161 tcgttccggc aacagtcaat tcgatatctg tttgttctgc ttgttctact gttggattca
     8221 agacgtactc gccatttaca cgaccaactt ctacgcctgc gattgggccg tcaaatggga
     8281 tatctgagat tgatagtgct aaagaagaac ctagcattgc tgccatagct ggcgagcagt
     8341 ctgtttctac actcatcacg atgttcgtta cttggacttc attacggaag ccttctgcaa
     8401 acatcgggcg aattggacgg tcgatcaaac gggcagtcaa tgttgcttcc gtacttggac
     8461 ggccttctcg tttgatgaaa cctccaggga ttttccctgc tgcatacatt ttttcttcat
     8521 aattgatcgt taatgggaaa aagtctgtat cttttgcttc tttagaggcc acagctgcac
     8581 ttaagactac tgtatctcca taacggacta aaacggctcc gtttgcttgt tttgctagct
     8641 gaccgacttc gatttgtaag ggacggccgc cccatgtcgt tttgaatact tgtttttctg
     8701 acataacttc tccttttcct tctcgtgaaa gcaagacgct actaaacaaa tgaaaactgc
     8761 cgaaagccgt cttttgagca gctttcattt ggttagtagg agtccatcta gcttgatcac
     8821 ggaattttac ttaattgata tacgagtaaa aaaagtgaga ttcatgtgaa tcccactctt
     8881 tttgactaaa attaacgacg caagcctaaa cgtttgatca attcgcggta acgttgaacg
     8941 tcagttttac gtaagtaagc taacaagtta cggcggtgac caactttttt cattaggccg
     9001 cggtatgaat gatggtcttt tttgtgcacg cgtgcatgtt cattcaagtg gttgatttct
     9061 tcagttaata cagcgatttg tacttctgga gaaccagtat ccccttcatg gcgagcgtat
     9121 tcgttgatga tttcattctt gcgttctttt gaaattgcca tttctttcac ctctttcgat
     9181 tattcccctg cgactgagta aaacgttggt gattcgctta accaagtggc aggtcatgtg
     9241 cctagagcac acagctatac tttacctgaa accgggttga aaatcaagta gttaatcatt
     9301 aagaaaaggt agttttcaaa caatcttact actacttttc catttttgtg gtaaactata
     9361 atgcgaaaat agacttgaaa cacagtcttt taagaaaggg gtacatattc catgattaca
     9421 atggatgata tcattcgtga aggaaatccg accttacgcg aagttgcaaa agaagtttct
     9481 cttccattaa gcgaagaaga tatctcttta ggaaaagaga tgcttgagtt cttaaaaaac
     9541 agccaagatc caatcaaagc tgaagaacta cacttacgag gcggtgttgg cttagctgct
     9601 cctcaattag atatttctaa aagaatcatt gctgttcacg taccaagtcc tgacccagag
     9661 gcagatggac cttctctaag tactgttatg tataatccta aaattttgag ccattctgtt
     9721 caagatgctt gtttagggga aggtgaaggc tgtttatctg ttgatcgaga agtaccagga
     9781 tatgttgttc gccatgccaa aatcactgtg tcttattatg atatgaatgg tgaaaaacac
     9841 aagattcgtt tgaagaatta tgaatcgatc gtcgttcaac atgaaatcga ccatatcaac
     9901 ggtgtgatgt tctatgacca catcaacgat caaaatccat ttgcactaaa agaaggcgta
     9961 ttggtgatcg agtagagaat tggcaggttc cttcgatact atgctacaag aaagctttat
    10021 aaaaaagggg agtgctcaaa agctgcactt ccctttttta tgtttctcca ctaatcgaaa
    10081 tttagataga catctagaaa tcgcaattcc aaaaaattct gtgtattgga tacgcatctt
    10141 ttcttatttt ttcttatttc ttccatatgg gtgtttatct cagagatgat agaatagttt
    10201 ctaggataaa aagtagcagc tacgatgtta tccttcgtag aggattcctg cttcagctag
    10261 tttttgtttg atgtttttca gcatatgctt ttctgggtag cggatcgtat gcagatggat
    10321 gccgcctgtt aattcggata acaaagtagc attacttttt tgataacgtc tcataaagga
    10381 atcgatatct tgtggcgttt tgatgtgcaa agttcctgct aactcaccgt aaaggggatg
    10441 ttccacaatt acatcaatga tttcaccacc attttcaacg attagtctca gttcttcttc
    10501 tgtttgatct tttccatgct ggacagcgat tttactgatg tgcccagtat tggtttcttc
    10561 tgtttcgagg atataacctc ttgctgttgc cacgatactt ttacctgcag ctcgtagtaa
    10621 agcaatatcg cctacaatga tctgacggct gactccaaaa gttttcgcaa acttgctagc
    10681 actcactggt ttttctgtat ttgccaaggt ttgcatgatt tgttttcgcc gtgtctctcc
    10741 ttcgatcaca cttattcctc cttcattttg ttttggtatt cgcgcgagtg tttcatcaaa
    10801 tcaacatacg tatctgttat tgtttcctta tatcccttat ttttcagcag actaacaact
    10861 ttatccaata cttgctgctc tctttttgca tcataaactg cttggttttc ttcttttttc
    10921 acttgtccta ttttagttac cgtatccatt cttttttcta ataaagcaat caactctcga
    10981 tcgatcatat cgatcttatg acgtaatgtt tcaagagact cttctgttag ttcttcctct
    11041 actgtcttga tgatacgagc aggatttcct gcaatcacta cattgtcggg aaaagatttg
    11101 gtcacaaccg aacctgcgcc gactactacg ttgtccccta gagtgacacc cggcgtgacg
    11161 ataactcctc cgcctaacca aacattatta ccgattttaa tgggttttgc gtactccaga
    11221 ccgctgttac gtttcactgg atgtagtgga tgcgtcgccg tgtaaagctg gacattcgga
    11281 gcgaacatgc agttatcacc gatttcaatc gtgctgacat ctaaaaatgt acaattgaaa
    11341 tttgcataaa aattctcccc aacaaatata ttatatccgt aatcaaaatt gataactggc
    11401 tccatataga tttttttccc tgtacggcca aaagtctctt tcaacaattg ggatcgcagt
    11461 tccgctgatt ctgcttggtt gatgatttgg ctttgacttc ttgcaaacat acggtcggca
    11521 accagctcag gatcattggc aaaataaagc tcgcctgcaa tcattttttc tttttcggtt
    11581 ttgaattcag tcataatctg ctcctaattc ttgtattttt ttgaaaacaa ccgctccgtc
    11641 ttcttgaata aacgtataag gctccgctaa ataaacaggc gtttgattga ttactaagat
    11701 tttggctgac ggctggcgaa actgtaccag atcacaaaac ggatgaactt gaaaactcgt
    11761 tccaacaatg acgatcagct ctgcttggct gacggcataa gccgcctgct ccatgactcc
    11821 atcttgaaac ccttcaccat ataacacgat gtctgggcgt atttgtcctc cgcatgcttg
    11881 atgtttgtcg ctgtgaagat attctttcca gtctaccgtt tgttgacatt ttcgacaatg
    11941 acattgatac aaacttccat gaaaattgac gagttgcggg cttttagcct ttgcgtgcaa
    12001 accgtcgatg ttttgggaaa caacccaaac atttttttct ttcgctagat gtgccatttc
    12061 caaatgaatg atattgggtt ttgcttcagg atgataaatc tttttgacaa attgatagaa
    12121 tttatccggt tcattcacca ttgcttgatg actcagcaag tattccggct gctccatccc
    12181 gtggtagatt cctgacaaag atcgatagtc tggcactcca gaaggtgtgg atacccctgc
    12241 accggtcaaa aatgtaattt gctgataagc taatatctct tttgctgctt cgtctactgt
    12301 taattgtttc atgttttgtc acctcacttt ccattatagc ccatactcga acaaatttat
    12361 tgttgaaaag tcagaaaaat gggaataaaa aagtgttatg ggcatttgaa caatttatca
    12421 caatgttcac tgacaaaaga atgaaaacgt gttacaatac tgctgtataa aagtgaggtg
    12481 aatcgatatg ttaccattct ttatttattg gggcgttatt ctggcttgta tcgcctggtt
    12541 agcgttaagc gtttatttct ctgtttttta tcttgtacgc aaagaaaatg gaaatctttg
    12601 ggcttttgct ttattcaacg tgattgccgc aattgtttta gccattacat tagccgtata
    12661 tcgaacatgg ggctggggaa tcacccaata ctcaagtttg atttatctaa tattagccat
    12721 ttatggcgtc gttgttcttt tgcaagcgat cctaggacgt gagccaaaga aagcggcagc
    12781 ttaaatgatc gagatggaac tggacacagg aaattttctg ttctagttct ttttttattg
    12841 tattacatac atttttgtgt ataattatac tatcgtttgc aatggataga tgattcacat
    12901 tttgattgtt ttttcggtaa aagtaaagat ttctatattt atcagtattt tttcaaaaaa
    12961 cagcagttat agattgacaa agcctctcga gtatcgtaaa ctttttttgt ataaataact
    13021 gaatacgaga tgcctcaatc aatgaggtag aggtcgcgag aatcattaac cctgtggagt
    13081 gaagcaaaca tggagaagca ggaataggga ggatcgccga aatgtgcact tttttgcttg
    13141 gagtgtatgt tgggacgcaa gttaagagct tgcggactgt cttagcagtg atgctaagtt
    13201 gcgctatgtt ttgtgagagg tttatcacct tatggaattt tagcgccctt tttatatcta
    13261 taaatcggtc gctttttatt ttttcagata aagctttacc gcatttcatc tagaccgctc
    13321 gtgtatcata taatcagaaa gggagaaaaa aatgaaaaaa aatatggttt cagttgccgt
    13381 actatttgct tttgtcttct cactattcag tgtgggatct ctggcaaatg cagatgaaaa
    13441 aacaccgagc gatgagtttc gcgtaggtat ggaggctggg tatgcaccct tcaactggtc
    13501 tcaacaaaca gatgagaata atgccttgcc aattcaaggc caaaatagtt acgccggcgg
    13561 ttatgatgtt caaatcgcta aaaaagtagc agatggttta gggaaaaaat tagtcatcgt
    13621 ccaaacgaaa tgggatggtc tggctcctgc ccttcaatca ggcaagatcg acgcgatcat
    13681 cgccggaatg agccctactg ctgaacgacg aaaagaaatt gattttaccg atccttatta
    13741 cgaatcacaa ttagtagtag tagttcaaaa agatagcaaa tatgcaaaag caaaaagttt
    13801 agcagatctt tctggagcaa agattactgc tcagttaaat acattccact atactgtgat
    13861 cgatcagata ccagatgtgc agaaacaaca agcaatggat aatttctcag caatgagaac
    13921 agctttagct tctggaatga tcgatggtta tgtcagcgaa cgtccggaag gtgtaacggc
    13981 aactagtgtg aacaaagact tggaaatgct tgaattcagc ggtgcagatg gcttccagac
    14041 aaacccggaa gatgttcaga tttctgttgg tatgcgtaaa ggcgacccgg atatccaaaa
    14101 agtaaaccag attctttctg gcatctctaa cgatgaacga accaaaatta tggaccaagc
    14161 agtcaaagat cagcctgctg caactgatag tgaagaaaaa cctggcatca tttctgattt
    14221 cactacgatt tggaaacaat acggcaatat gttcttacga ggtgctggac ttaccttgtt
    14281 catcgcattg atcgggacag ttgtcggtac tacacttgga ttactgattg gtgtcttccg
    14341 ttcggtacct gaatcaggaa atgcactgac tcgcttcttc caaaaaatcg gaaatgcact
    14401 tctttctatc tatgtcgaag tattccgcgg aacaccgatg atggtacaag caatggtcat
    14461 cttctacggt ctggcactcg cttttggcat ttcattgaac cgaacgattg ccgctttgtt
    14521 tattgtctct ctcaatactg gagcttacat gacagagatc gttcgtgggg ggatttttgc
    14581 agtagatgaa gggcagttcg aagctgcgca agccattggt atgacccatg gccaaacgat
    14641 gagaaaagtt gttattccac aagttttacg gaatatcttg ccagcaacag gaaatgagtt
    14701 tgttatcaat atcaaagata cagcagtttt aagtgtaatc ggcgtagctg atttgttctt
    14761 ccaaggaaac tctgcttccg gtgcgaattt ccaattcttc caaacattta cgatcgtcgg
    14821 gatcatctac ttagtcatga cctttacgat caccagaatc ctgcgggcgg tagaaaagaa
    14881 aatcgatggt ccttcagctt ataccaagct tgaagaaatc gacaattcaa atttgcagga
    14941 ataaggagga agaatcatga atgaaaaaat tattgaaatc aaacatctaa gaaaaagctt
    15001 cggtgaaaat gaagtattga aagatatcag cgtaacagtc aataaaggtg aagttgtgac
    15061 gatcatcggt tcttccggtt ctggtaaatc tactttcttg cgctgtatca atttactgga
    15121 aaaaccaaca ggcggcgaga tcatttataa tggagaaaat gtattagctc ctaaatataa
    15181 tttacctaaa tatcgaacga atctaggtat ggttttccaa tccttcaacc tgttcaacaa
    15241 tatgaatgtc ttggaaaact gtatgtctgg tcaaacaact gtcctgaaac gagataaaga
    15301 aactgccaaa aaagtagcaa tcgaaaatct ggaaaaagtt ggtatggatc gttatatcga
    15361 agccaaacca gcacaattat ctggtggaca gaaacagcgt gtcgcaattg ctcgggcatt
    15421 atcaatgaat ccagatgtat tgctattcga cgaacctact tcggcgttag accctgaaat
    15481 ggttggcgaa gtgcttaata cgatcaaaga cttagcacat actggcttga caatgcttat
    15541 cgtgacgcat gagatggaat ttgcaaaaga tgtatctgac cgtgtcattt ttatggataa
    15601 aggtgtgatt gcagaagaag gtacaccaga agatatcttt gtccatccga aagaagaacg
    15661 tacaaaagaa ttcctttcac gtattttaaa tgcttaggat caatagctga ttgggacaaa
    15721 gagctgcggt gtttaccgca gttctttttt atgaattact cataaaaggt aaatttctga
    15781 aaattttgga atcttagttg tgtttttcag aaaactcttt taatatggaa gtaattaaac
    15841 ttttttagtt aggatgattt aaaaatgaat gaagagcaag aactgcagcg tggactgaaa
    15901 aatagacatg tccaactgat ttctattggc ggagcgattg gaacggggtt gtttttaggt
    15961 tccggaaagt ctatccagct agctggccct tcgatattgc tcgcctatct tattactgga
    16021 tgcatctgtt ttttcattat gcgtgcgtta ggcgaacttc tgttatcaaa caccaataac
    16081 cattcttttc tggattttgt tgcagaatat cttggcaaaa aagcggcttt catcactggc
    16141 tggacgtatt ggttctgctg ggtatcgatt gcaatggccg atttaacagc catcggaatg
    16201 tatgtacgct actgggcgcc tggtgttcct cagtggcttc ctgaactgat tgcattaggt
    16261 cttcttcttt gcttgaatat ggtggctgtc tcattgtttg gtgagttaga gttttggttt
    16321 gcattgatca aagttgtagc gatcattgcc tttatcctcg taggagctta tatgatcctc
    16381 acccactata cgacagatat tggcacagcc agtatcacga atctatggtc tcatggcgga
    16441 ttctttccaa ctggtgcaaa aggatttatc ttagctttcc aaatggtaac ctttgctttt
    16501 gctgggatag aattagttgg attagtcgct ggagaaacag aaaatcctga aaaagtgttg
    16561 ccggaagcta tcaataatat tcctatcagg atcatcttat tttacttagg atctcttttt
    16621 gtcattatgg caatctatcc atggaacagc ttgaatccag ataacagtcc atttgtagaa
    16681 gtattttcgg aaattgggat cacgattgct gcttccttga tcaatttagt cgtattaagt
    16741 gcagcagctt ctgcctgcaa cagtgcaata tatagtacag gacgtatgct tcgttcttta
    16801 gcgcaagaag gcagtgcccc taaaaaattc aaaaagttaa caacacatca tgtgccaggg
    16861 aatgctttga cattttctac tatcgtcatt ttcatttctg ttatcttgaa ttacgtgatg
    16921 cctagtgaag tattcacact agtaagcagt attgccacta cttgtttctt gtttatctgg
    16981 agtatgcttg tctacacgca catgaaatat cgtaaaagta tcctcggcaa aaaagctcat
    17041 tcatttaaaa tgccgttgta cccatttagt aattatctgg tatttcttta catggccttt
    17101 gtgtgtgtcg tcttgttcct aggaaaagat acgagaatcg ctttattact aacacctgtg
    17161 tggttcttgc ttctattact aatctatcat atgaaatatg aacgtaagaa gtaagaaagt
    17221 agttgtgaca gaagtggtca gcttcgagaa ataagaagga atttacgaaa attgcttctt
    17281 aaatttttgt gaaatttcgg cttatttctt ggagttaaaa gcttctgtcc cagcctcttt
    17341 tctatttccc cggaaacaac cgctgataat aggcatctgc ttttttatag gaaaaaatcc
    17401 aaataccaga tacaaaaaaa cgtccgaata acccgatcaa cggtacaaaa aagacaccgg
    17461 caatcaagca taagttaatc atcatggaga taagacttcg acggtccttt gacaaaactc
    17521 tcacgacatg ggaatctggt ttatttgctt tcatgatttg cctcatcaag ataccaaaag
    17581 aaaaactcca catcgtgtat actaagacat aagccatctc aggccaaaaa gcttcagggt
    17641 tacgcccgac ccaactagta acagaaggac ataaagtcag ccaaaagagc aaaaagatat
    17701 tcgaccagac cactcggtaa ttaattcgtt ctgccatctg aaatatacga tgatggttca
    17761 cccagatcac cgcaacaaat ataaagctga tggtataagc taataatgta tttcggatga
    17821 cccataaaga atgaaagcta gatgtattag ggatcttgat ctctaataca agaactctca
    17881 aaacaatggc taccactcca tcagtaaatg cttctaatct cgactttgtc acgatgattc
    17941 ctcctcagat gattcagcgc ttttatttta tcacttacgt tattaaataa ccgtcgtttt
    18001 atataggaaa ttttctgaaa attgttgcat atttttcgaa attttgttac ttttaaaaaa
    18061 accgtaaagg agttatgtat gacaaaaaaa ttatcattta aagactatct ttttataggc
    18121 tccatgcttt tcggtctatt ttttggtgct ggaaatctta tctttcccgt tcatttgggt
    18181 caagaagcag gcgctgctac cttttgggcg aatctaggtt tcttggttac cggtatcggt
    18241 cttcccttct taggggtgat tgccattggt gtatcgaaaa gctcaggtgt atatgaatta
    18301 gcaaaacgga taaatcaaac atatgcgatg gtttttacca tcttgcttta cttagttatc
    18361 ggaccgtttt ttgctttacc tcgccttgca accacatctt tcgagattgg tttagcacca
    18421 tttatcgatc ctagcagaca gacgctatat ctagcaattt tcagtattgt attttttgtg
    18481 ctggcttggt ggttttcaag aaaaccgaca aagatattag attatgttgg aaagttcctg
    18541 aatcctgcat ttttggtttt attaggtgct ttgcttctcc ttgctttttt gaatccacta
    18601 ggttctgtaa atcaagcgcc tattcagcca aattatcaag agcatgcttt tttcacaggg
    18661 tttactcaag gatataacac attagatgcg cttgctgctt tagcatttgg gatcatcatt
    18721 gtcacaacaa tccaaaacat gggtgtaaca aaacctgctg aaatcgctaa agacacgatc
    18781 aaatctggtg cgatcagcat tatattgatg ggaatcattt atactttgct cgcttattta
    18841 ggtgctatga gcttaggtag ctttgctcta agtgaaaatg gcgggatcac gctggctcag
    18901 attgctgatc attatttagg tacttatggc agtatcttat tagcatttat cgttatatta
    18961 gcttgcttga agaccggtat tggtttgatt acagcttttg cagaaacatt cacagatttg
    19021 tttccgcaaa gaaattacgc attctttgtt gcacttgcta gtatccttcc ttgtttagcg
    19081 gcaaacgtag gcttgacgaa tatcattcaa ttttcgttgc ctgttttgat gttcatctat
    19141 ccattagcaa tgacactgat tttactagtg ttagttggtc ctttgtttaa gcagcgtacg
    19201 gctgtttatc gaatgactac ttattttaca ttgattgctt cgatttttga tgggttgaac
    19261 gcttgcccag aaactatcaa acaaacacct attgttcaaa atcttttata tgttgcggaa
    19321 agctacttgc cgtttttcaa attggggatg gggtggatcg taccagcagt tatcggtttt
    19381 gttatcggac tgatttggag tttcgctaag aaagaagaag tagcagattg ataaaagagg
    19441 ttgtgaaaga gacgttcagc cttattgccg ataggctgtc tcttgaacac tgtttattga
    19501 gaggtcgtga aaaagccgct cagtttcgag tattaagaaa tattttttga aaatggcttc
    19561 tcacattttt gcataaattt gacttaatac cgagaaactg gcttttgaac accgtttatt
    19621 gagcgtctat aaactgacct gagtattaga gaaacaaaac ggcaaagtag ctccatata
//
LOCUS       DAZKLD010000043        19596 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.043, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000043 DAZKLD010000000
VERSION     DAZKLD010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19596)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19596)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19596
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.043"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(334..696)
                     /locus_tag="ACG8KR_001248"
     CDS             complement(334..696)
                     /locus_tag="ACG8KR_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0200628.1"
                     /translation="MASKRVAGTIMLHLEDGTKKFLVHSIDDKLEFALAELSEGKTGL
                     ANILNFLKEIVHIDVSKISLMELTNTHIKEENVPLFVFETEQKNQREELGEGYIWEEP
                     GQMRSVLGTYEVEGVPFF"
     gene            complement(770..1150)
                     /locus_tag="ACG8KR_001249"
     CDS             complement(770..1150)
                     /locus_tag="ACG8KR_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvfD/Ygs/GSP13 family RNA-binding
                     post-transcriptional regulator"
                     /protein_id="HFQ0200629.1"
                     /translation="MTYKIGQIIEGKVTGIQPYGAFVSLDNETQGLIHVSEVQSGYTK
                     NIHSLLKVGQPVTVQIIDIDEYSKKISLSLRTLEKTNPAVPYRRKRYFTNKNRKIGFT
                     TIAEQLPIWIEEALSELERKENKK"
     gene            1232..2407
                     /locus_tag="ACG8KR_001250"
     CDS             1232..2407
                     /locus_tag="ACG8KR_001250"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047804 - cysteine-S-conjugate beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MalY/PatB family protein"
                     /protein_id="HFQ0200630.1"
                     /translation="MIEFDKQISRKDTESVKWDAIAETYQMDDLLPLWVADMDFLSPG
                     GVAKAFSEYIKHGIFGYATLSDKLYQAIIHWERQHHAVELTKENIVFTSGVLTSLAAA
                     VQTFTKPGDSILIHDPVYPPFSSIIETNQRHIVRSSLLEKNNHFIMDLSDMEKKIEEN
                     NVKAMILCNPHNPGGRVWSKEELKQLSELCLKHQVILFSDEIHQDLTLFDHTFTSMLT
                     IDPNLDDLLVAFTSATKTFNLAAIKNSMVFIKNPEMKADFEKHLVMNQQHEINTFGLI
                     GTQAAYETGGEWLSQLIPYLEKNVQTVNQFFEQYLPKVRIMNPEGTYLMWLDFTAYIS
                     DDQELENTLVRKGKVVLNPGITFGPSGHGHMRLNIACPEETLLEGLNRIKYALETID"
     gene            complement(2484..3068)
                     /locus_tag="ACG8KR_001251"
     CDS             complement(2484..3068)
                     /locus_tag="ACG8KR_001251"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFQ0200631.1"
                     /translation="MAFPQLDLKSEKGPKAVIKTNRGDITVQLFPELAPKTVQNFIEL
                     SKKGYYDGVIFHRVIPDFMIQGGDPTGTGMGGESIYGESFEDEFSRELFNLRGALSMA
                     NSGPNTNGSQFFIVNNTNVPANMLGQLEGAGFPSEIIEAYKGGGTPWLDFRHTVFGHV
                     LEGMDTVDEIANVQRGPQDRPVHDVVIETIEISE"
     gene            3269..4267
                     /locus_tag="ACG8KR_001252"
     CDS             3269..4267
                     /locus_tag="ACG8KR_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFQ0200632.1"
                     /translation="MEIYDITIIGAGPVGMFAAFYAGMRQAKTKIIDSLPQLGGQLAT
                     LYPEKYIYDIPGYPAIKASELIDQLEKQLTTFNHTFHLKEEVLSLTREDEVIEITTNK
                     GIHYSKAVILALGNGSFQPRKLNLDNAESFENHGLDYFVNDLMKYAGKKVAIAGGGDS
                     AIDWTLMLEPIASEVYLIHRRPEFRAHEHSVSRLKSSSVNLLTPYLIDGLSGNNGELT
                     DIRLKKVKSDETIDLMIDSLIVNYGFSSNLEHLSSWGLDSTRNAITVKSDMSTSIPGV
                     YAAGDICTYEGKVKLIATGLGEAPTAVNNALHYINPKERTQPGHSTSLYDKNLRPS"
     gene            4379..4888
                     /locus_tag="ACG8KR_001253"
     CDS             4379..4888
                     /locus_tag="ACG8KR_001253"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HFQ0200633.1"
                     /translation="MVVKTETLEEKARELLKERGVTVEDIAQLVMFLQKDYISDLTLA
                     DCTESVNSVLTKREVHNAIITGIQLDILAEKKKLMSPLQHIIEDDEGLYGIDEILALS
                     IVNVYGTIGFTNYGYIDKVKPGILKELNSHDGVHVHTFLDDIVGAIAAAAASRLAHKE
                     PEKRSRLTQ"
     gene            complement(4918..5577)
                     /locus_tag="ACG8KR_001254"
     CDS             complement(4918..5577)
                     /locus_tag="ACG8KR_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HFQ0200634.1"
                     /translation="MKNSRWQWMEYAGLFSLFLTLISLAVGVTINFRPLYVFDIGHLQ
                     ILDYTSLDQETLLKNFDHLMNYLNNPFKTILSLPDFPVSASGAHHFYEVKILFLVDYA
                     VFFITLIPSILFIKYLQKNDRLWRLIRPFQIGMLLPVVFGFFMMIGFDRFFILFHETF
                     FNNDDWLFDPVTDPIINVLPEEFFMHSFILFFVLLELFFAVFLFLGKNSLKQTKKKEL
                     V"
     gene            complement(5579..6343)
                     /locus_tag="ACG8KR_001255"
     CDS             complement(5579..6343)
                     /locus_tag="ACG8KR_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HFQ0200635.1"
                     /translation="MDYKGYLIDLDGTIYRGTQPIPAGKRFVEELQKRKLPFLFVTNN
                     TTKSPETVANRLADEFDIHVAPETVYTATLATIDFMKADGKGKKVYVIGEAGLIDLIL
                     AAGFTWEEEAPDYVVVGLDNYLTYEKVVKATLAIQKGATFIGTNPDKNIPTERGLLPG
                     AGSVISFVETATQTPPIYIGKPEAIIMDKAVEVLGLQKEEVIMVGDNYETDIQAGIRN
                     NIDTLLVLSGFTKEEDVPGLPIPATYVKQSLDEWSF"
     gene            complement(6351..7028)
                     /locus_tag="ACG8KR_001256"
     CDS             complement(6351..7028)
                     /locus_tag="ACG8KR_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HFQ0200636.1"
                     /translation="MSAKQNEQQNVEHKTEIAKKKSSAEQETTVTEELTAALEEIVEE
                     PKAEKVKGELVTLLDDSNFLIGERHYRLVSDYREGFNAEKLGERYSDVLARYDYIVGD
                     WGYEQLRLKGFFQADNRRAHPDQRIDSLEDYLYEYCNFGCAYFVIERIGGKKEKTTQR
                     RKKKKNHNRAYIDEKKGPVASNRKKPVIKNRKTESAKTSEKVKKQEQPKQVEKTKTAF
                     TIRKREE"
     gene            complement(7065..8450)
                     /locus_tag="ACG8KR_001257"
     CDS             complement(7065..8450)
                     /locus_tag="ACG8KR_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HFQ0200637.1"
                     /translation="MEKIVILHTNDLHSHLENWPRIRRFLDQRKRENEKKEKTTTIAV
                     DLGDFVDRWHPLSEATNGQENILLMNEVGYDAATIGNNEGVGNSKEELNHLYDHAEFD
                     IILDNLFDKNTLQPPAWTKPYKIITTKHQTKIGLIAFTAPFPLTYNPNGWDIRNPYDI
                     LPELVEDLRSQVDVLVLMSHLGIQDDFQIAKEIPAIDVILGSHTHHLFREGHVINDVQ
                     IAAAGKYGQYVGEVHLTIDEEKKILKHSAKAIPTETMTAFTEDEQEINSYLERGHELL
                     KEKEVADLPFSLSLDIFNEHSFIQVAFEAVKERGKTEAAILNSGLFLTEIPKGRVNQD
                     QLHTALPHPMHLLNVTLGGNDLIRLVLEIEKNRNFLRNYPMKGMGFRGKIFGQMVYNG
                     ITYDSINHQVFWKNKPIDLNKNYTFTTVDHLMFVPFFPTIEIAGKNEFLFPEFIRSVV
                     GDYLKAHYPIK"
     gene            complement(8660..8744)
                     /gene="ffs"
                     /locus_tag="ACG8KR_001258"
     ncRNA           complement(8660..8744)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="ACG8KR_001258"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(8767..9282)
                     /gene="tadA"
                     /locus_tag="ACG8KR_001259"
     CDS             complement(8767..9282)
                     /gene="tadA"
                     /locus_tag="ACG8KR_001259"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HFQ0200638.1"
                     /translation="MVNTNLDAVEKEKWMRLALAEAKKAEMLHEVPIGAVVVLDGKVI
                     GRGYNLRETTQDATTHAEMLAIKEACEKVGSWRLEDAALFVTLEPCPMCSGAMILSRV
                     AEVYFGAYDPKGGTAGTLMNLLEDERFNHQAYVEGGILEDECGMILTDFFRKLRQRKK
                     KLKKDWSTNEK"
     gene            9389..10246
                     /locus_tag="ACG8KR_001260"
     CDS             9389..10246
                     /locus_tag="ACG8KR_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0200639.1"
                     /translation="MKMDKLLALYPQAQKKNSPASEADILSFAIDDAFIWIKQDSLSQ
                     QETSLLKALFPVINDQKKHPWYHYLFKDASCVQEKSFRLIQLHVESKGEFLKKEWQDT
                     ILELFRYSADFFFYTDTDALLVEQINRDYLDATEINGIFLSLDADFDTTTSVFVGSFH
                     SPGTDLVPLFAEERKIFLTEQNNLYTHSRTFSMQDVALHYYTKDTVKDSKLIKSYIEL
                     IKQNEMEVIIQELWNELGNVSSAAKTLFLHRNTLKYRIEKFQEQSGFNLKKANDLLFC
                     YLLLLHEHN"
     gene            complement(10327..10662)
                     /locus_tag="ACG8KR_001261"
     CDS             complement(10327..10662)
                     /locus_tag="ACG8KR_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0200640.1"
                     /translation="MKTSTKVTIGLSVAAAASVATAVVVSGKVMEKIHHMTTRTKVKK
                     FVYDKFDGNEKLLEIVDGLSDSDLDSLMGMLAKIKSGKKKISVYGDSIKDSTEDVKDR
                     LMHFVEKMM"
     gene            complement(10729..12099)
                     /locus_tag="ACG8KR_001262"
     CDS             complement(10729..12099)
                     /locus_tag="ACG8KR_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmF rRNA methyltransferase first C-terminal
                     domain-containing protein"
                     /protein_id="HFQ0200641.1"
                     /translation="MKEEATTLPQQFIKKYRLLLGEEASDFFSALEQGSVKKGFRWNP
                     LKPAGLDMVQTYHSEELQPAPYSNEGFLGTVNGKSFLHQAGYEYSQEPSAMIVGTAAA
                     AKPGEKVLDLCAAPGGKSTQLAAQMKGKGLLVTNEIFPKRAKILSENIERWGVSNAIV
                     TNHAPAELVPHFSGFFDRIVVDAPCSGEGMFRKDPNAIKEWTEESPLYCQKRQQEILS
                     SAIKMLKNKGQLIYSTCTFAPEENEEIISWLVENYPVTIEEIPLTQSVSSGRSEWGSV
                     AGLEKTIRIWPHKDQGEGHFVAKLTFHGQNQMHKEKKTRKKSKVQMTKEQEKLWTEFS
                     NDFHYEATGRLLVFNDHLWEVPELAPSLDGLKVVRTGLHLGDFKKNRFEPSYALALAT
                     KKIENIPCLPITQKEWQSYTAGETFQRDGNQGWVLLVLDKIPVGFGKQVKGTVKNFFP
                     KGLRFH"
     gene            complement(12177..12713)
                     /locus_tag="ACG8KR_001263"
     CDS             complement(12177..12713)
                     /locus_tag="ACG8KR_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFQ0200642.1"
                     /translation="MMGRVTNYSKEYLLFKSMVYVEEYGMKSITARELAEFCGCSTYP
                     IYTHFKSISGLKEKILEEITVYFENYLAECSSDDVLSIVYLLKDFFSIHESIREIIAK
                     SDLDMDSLFKRPFFSYVKGHTKIKEEKSLELVWLNALGSLRSDDSSQDFLEMMNAVWD
                     KNEVTDEYLENILKEQSP"
     gene            13022..14503
                     /locus_tag="ACG8KR_001264"
     CDS             13022..14503
                     /locus_tag="ACG8KR_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0200643.1"
                     /translation="MLIEARKKELLVLQMVIQKNNCSLQELSNDLHIPKRTIKELIRK
                     INVSLKQFLLIDEFIYSNHKGEIQTKETDQTKKLAVFSKLKLCYLKESNRFNYLMLLI
                     NYPQTAVPKKYLLEQLYISPSYLEKLTKQLNHSLKKFHLRILSVKNCCTLEGNELSIR
                     LYLYFFLSDTFGGNDWPFSSLELNALKMNSTDEQIEKISKQEKNIYLLLTLFILRNKQ
                     QAYFPKTSSEITELMDLLQHTHDYSEYFTSFSVSRPCEETETERAYFNYFFYCFFPNQ
                     ISFRQKQEIGFLFSTSKNLICRQVGQLMTYLTQQFPLNHLENKIPIYFYFLVLAVVFF
                     ELLGESSSSFSTLCFPEATSNHSSSFIQEKKLREKIDTIFSKNGERNQISQYLSQLIQ
                     HLLQTETKEQLLIHLQITKQFTGNYHLQSHLQQLFRKDAVKITAYSEHADLIITDQHE
                     KISDETETFYLDTTHNTVLFGKLIKAVHELYMKKGYENKTKRM"
     gene            complement(14606..15313)
                     /locus_tag="ACG8KR_001265"
     CDS             complement(14606..15313)
                     /locus_tag="ACG8KR_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HFQ0200644.1"
                     /translation="MYGYGMYGFLDPTFLLVILGLVISGIASAYVNSTFRKYDQVRSK
                     NNVTGTQAAQYILQSQQINNVGVQQIAGDLTDNYNSGNKMLSLSQATAQSTSVAAIGV
                     AAHECGHAVQDASGYVPLKIRAALVPAANFGSMISFPLIMVGVLLSWNSTLINIGIFA
                     FSLALLFQLVTLPVEFNASRRAIQILSEGGLLTEEEVPMARHVLFAAALTYVAAALST
                     FLQLLRLILLFGGNRRD"
     gene            complement(15392..15730)
                     /locus_tag="ACG8KR_001266"
     CDS             complement(15392..15730)
                     /locus_tag="ACG8KR_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein YjdM"
                     /protein_id="HFQ0200645.1"
                     /translation="MTLPNCPECGSEYTYEDRGLLICPECGNEWNKEEQETVIEDGLV
                     VLDANGNRLLEGDSVTVIKDLKVKGASGAIKQGTKVKNIRLVEGDHNIDCKVEGFGPM
                     KLKSEFVKKN"
     gene            complement(15841..16803)
                     /locus_tag="ACG8KR_001267"
     CDS             complement(15841..16803)
                     /locus_tag="ACG8KR_001267"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HFQ0200646.1"
                     /translation="MTVQQEIVDTIKKYDKIIIHRHLRPDPDALGSQVGLAELLRESF
                     PEKEIRQVGETIEGLRFLAEMQEVEDVFYQGALVIVTDTANSPRISDQRYQLAEKLIK
                     IDHHPNDDPYGDLVWVNTKASSCSEMIAEFALMFPESLKMNVKAARLLYAGIVGDTGR
                     FLYPSTTSRTLEIASILRNYPFDASALNREIEQMPMKVAKLSGYLYQNIQVDENGAGR
                     IILPQEILDKYGIDDSETASIVSLPGVIDEVLAWGIFVQQPSGYYRVRLRSKGPIINE
                     LAKRHHGGGHPLASGANAKDLAEVEEIYKEIQDICQSYKSRKTE"
     gene            complement(16818..18149)
                     /locus_tag="ACG8KR_001268"
     CDS             complement(16818..18149)
                     /locus_tag="ACG8KR_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DRTGG domain-containing protein"
                     /protein_id="HFQ0200647.1"
                     /translation="MATKHDLILEYIESLPVGNRISVRSIAKELNVSEGTAYRAIKDA
                     ENVGLVSTIQRVGTIRIERKLKKHIERLTFGEVVRIIEGDVLGGAAGLDKVLNKFVIG
                     AMTKHAMERYITPGSLMIVGNRAEVQKLALEDGAAVLITGGFNTTAEIAKLADDLEMP
                     ILRTSYDTFTVATMINRALSDQLIKKDIMLVSDIYMPAEKTHYLQQTDTISDYQRLSE
                     ETQHSRFPVVNRHHRLMGIVTAKDVLGKSPNQIIDRVMTKEPISVKKTMSIASVSHQM
                     IWDGLEVMPVVSDDLTLEGLITRQDVMKAMQLVQRQPQIADTISDQISGNIHTIDTDR
                     EGNRLENPKFKFVVAPQMVNGVGTISFGVLSEIISDVAQKTMVMNQKRNILIEQVNLH
                     YLRLIQLESELDIRPRVLEIGRRSAKLDIEVFIENTIVSKAIVVCQVMERS"
     gene            complement(18157..18837)
                     /locus_tag="ACG8KR_001269"
     CDS             complement(18157..18837)
                     /locus_tag="ACG8KR_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFQ0200648.1"
                     /translation="MKITYHGHSCLTIEMENGQRVMIDPFITGNPLTDLTAEEVKTDW
                     LLITHGHSDHIGDMIPIAKENQATIISIVEIADYANSRGVDSFGMNIGGKHAFPFGVV
                     KFVHAQHSSSYEVDGIVQYMGEPSGIIIQAEGKTIYHAGDTAYFSDLGLLAEEFDIDV
                     AFLPIGDNYTMGPEDAAKAAQTIRAKKVVPIHYNTFPVIEQDPESFVELLEDSEGKIM
                     SVSETLEV"
     gene            complement(19056..19535)
                     /locus_tag="ACG8KR_001270"
     CDS             complement(19056..19535)
                     /locus_tag="ACG8KR_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HFQ0200649.1"
                     /translation="MMSQTKVERYLEEQKISYQPLDFSHVVEESFTEELQKRGIDPAL
                     ICKTLVVKGNKTGVIIALIPLNDHLDYKKTRQVTKDRKVGFPGMDFVLMHTGYPHGAN
                     TPIGIKLAHPDYCFLADSSLKEKEEVIVSSGEIGRSLRIKVADLEKIVHPIYADLIS"
BASE COUNT         5846 a         4032 c         3242 g         6476 t
ORIGIN      
        1 aacgactcag ttatcttatc atgtcgttag taacatgtca ataactattt tttaaatttt
       61 ttagaaatgt gatataacat ccacttcaga atcaacgctg ttaaatttat catgtatttc
      121 ttccttggtc aactataaat taagcattac tttgtgaaat gatttttcga tcaattatta
      181 ttattcgtta tacaaccgtt ttcaacatat tttgaaaata aaatgcacta aaaaactatc
      241 tttagcagaa catttcattg tattttttgg accaaacaat aaaaaaagaa gtttgaaaaa
      301 aattcaaact tctttgttcg cttaatctgt attttagaag aaaggcactc cctctacttc
      361 gtaagttcca agaacagagc gcatttgacc tggttcttcc caaatatatc cttcgcctaa
      421 ttcttctctt tgattctttt gttcagtttc aaagacaaac aacggaacat tttcctcttt
      481 aatatgtgta tttgtcaatt ccatcaaact aatcttgcta acatcgatat gaactatttc
      541 ttttaagaaa ttaagtatat tcgctaaacc agtctttcct tcagaaagtt ccgctaacgc
      601 aaattccaac ttgtcatcaa ttgaatggac caaaaatttt ttagtaccat cctccaaatg
      661 caacataatt gtaccagcaa ctcttttgct tgccaaaatt tccacctcga tcaacttgtt
      721 ctatggctta cataaaaatt ctagcacatt ttcagaaaaa atacactttt tatttcttat
      781 tttctttccg ttctagttca gataatgctt cttctatcca aataggtaac tgctccgcaa
      841 tagtggtaaa tccgatctta cgatttttat tcgtgaaata cctttttctg cgatatggaa
      901 cagctggatt tgttttttct aacgttctta aagataaact gatttttttg gaatattcat
      961 caatgtcgat gatctgaact gttactggct gtccgacttt taacaatgaa tggatatttt
     1021 tcgtataacc ggattgtact tctgaaacat gaataagtcc ttgcgtctcg ttatcaagag
     1081 aaacgaatgc gccataaggc tgtatccctg tcacttttcc ttcaattatt tgtccaattt
     1141 tataagtcat ctacttccct ctcttcgcat ttatcattat actgtaaata gctcaaaaaa
     1201 agaagcgacg aaaatgactg gaggtcttaa gatgatcgaa tttgataaac agatttcaag
     1261 aaaagataca gaaagtgtaa aatgggacgc aattgctgag acatatcaaa tggatgactt
     1321 acttcctttg tgggtagcag atatggactt cttgtctccc ggtggggtag ctaaagcgtt
     1381 tagtgaatac atcaaacatg gaatctttgg ttatgccact ctgtctgata aactatatca
     1441 ggctatcatt cattgggaac gccaacacca tgctgtggag ttgacgaaag aaaatatcgt
     1501 ttttacaagt ggtgtgttaa ccagtttagc tgcagcagta caaactttta caaaaccagg
     1561 cgattctatt ttgattcatg acccagttta tcctccattt tcctctatta tagaaacaaa
     1621 tcaacgtcac atcgttcgaa gctctttgct agaaaaaaat aatcatttca tcatggactt
     1681 gtctgacatg gagaaaaaaa tcgaagaaaa caatgtaaaa gcaatgattt tatgcaatcc
     1741 tcacaatcct ggagggcgtg tttggagcaa agaagaattg aaacagttaa gcgaattgtg
     1801 tttaaaacat caagtgattc tgtttagtga cgagatccat caagatttaa ctttattcga
     1861 tcatacattt acttccatgc taacgattga tcctaattta gatgatctac tagttgcttt
     1921 tacttccgca accaagactt tcaatctagc tgcaatcaaa aattcaatgg tatttatcaa
     1981 aaatccagag atgaaagctg attttgaaaa acatttagtg atgaaccaac agcacgaaat
     2041 caacactttt ggactgattg gaacccaagc ggcttatgag acgggtggag aatggttaag
     2101 ccaactgatt ccttatctag aaaaaaatgt tcaaacagtt aatcagtttt ttgaacagta
     2161 tcttccgaaa gtgagaataa tgaaccctga aggaacttac ttaatgtggt tagattttac
     2221 cgcctatatt tcagacgacc aagaattgga gaacactctt gttcggaaag gaaaagtcgt
     2281 attaaatcct ggcatcacgt ttggtccttc cggtcatggg catatgcgct tgaatattgc
     2341 ttgtccagaa gaaactttac tggaaggact taatcgaatc aagtatgctt tagagacaat
     2401 cgattaaatc atttattcaa aataaaagga cgagacctta ctaggtttcg tcctttttca
     2461 taattactcc gtaccatacc aaactattct gaaatttcaa ttgtttcaat cactacatca
     2521 tgtacaggtc ggtcttgtgg accacgttgt acatttgcga tttcatctac tgtgtccatc
     2581 ccttctagta catgtccaaa gactgtatgt cggaaatcta gccaaggagt tccgccacct
     2641 ttatacgctt caatgatttc tgatgggaat ccagcacctt ctagttgtcc aagcatatta
     2701 gctggcacat ttgtgttatt tacaataaag aattggcttc cgtttgtgtt tggacctgaa
     2761 tttgccatag ataaagctcc gcgaaggttg aacagttcac gactgaattc atcttcaaaa
     2821 ctttcgccat aaatactttc gccacccatt cctgttccag ttggatcgcc gccttggatc
     2881 atgaaatcag gaataacgcg gtgaaaaatc acgccatcat aataaccttt tttggaaagt
     2941 tcaatgaaat tttgaaccgt cttcggtgct agttcaggga ataattgaac agtaatgtca
     3001 ccacggtttg ttttgataac agcttttggt cctttttcac tttttaaatc tagttgtggg
     3061 aatgccatat tgtttcctcc taatatttgc atacttcata ataataataa cataatacct
     3121 aacctctcgt aaaaatacta gatttgtttt cttttatttt tcatcactag atacattcta
     3181 taacttaccg tttcgatttc gaattcaaac atttttgaga agcattctca attaaattct
     3241 gctatagtgt gaaaagggag gaatttttat ggaaatctat gacattacaa tcatcggtgc
     3301 aggtcctgta gggatgtttg ccgcttttta cgcaggaatg cgccaagcta aaacaaaaat
     3361 cattgacagt ctacctcaac ttggtggtca attagcaact ttgtatcccg aaaaatatat
     3421 ttacgatatt cctgggtatc cagcaataaa agcaagtgaa ttaatcgatc aattagaaaa
     3481 acaattaact acttttaacc atacattcca tttaaaagaa gaagttctct cgcttactcg
     3541 tgaagacgaa gttatcgaga ttactacaaa taaaggcatc cattattcca aagcagtcat
     3601 tttagcttta ggaaatggtt cattccagcc aagaaaattg aatttagata atgctgaatc
     3661 atttgaaaac cacggattgg attatttcgt gaatgatttg atgaagtatg ctggaaaaaa
     3721 agtcgctatt gctggaggtg gtgattctgc tatcgattgg acgttgatgt tagagcctat
     3781 tgcaagcgaa gtttacttga ttcatcgtcg cccggaattt cgtgcgcacg aacatagtgt
     3841 atcacgcttg aaatcttcat ctgtcaactt gcttactccc tatctcatcg atggattatc
     3901 aggcaataat ggggaactga cagatatccg gttaaaaaaa gtgaaaagtg acgagacgat
     3961 tgatttgatg attgattctc tgatcgtcaa ttatggattc tcttctaatt tagaacatct
     4021 ttcttcttgg ggacttgata gtaccagaaa tgccattact gtaaaatcag atatgtctac
     4081 ttctattcca ggcgtttatg cagctggtga catctgtacc tatgaaggaa aagtgaaact
     4141 gattgctact gggctcggtg aagcacctac tgcagtgaac aacgctttac actatattaa
     4201 tccaaaggaa cgaactcagc ctggtcacag cacaagctta tacgataaaa atcttcgtcc
     4261 tagttgattt tttatatctc tttgcatttc aagttaaacc cgaaagcagt ttcttgcatt
     4321 tttttcatgt tccgttaaca tgaaatagag aaagaaaaaa taaaggggga gcaataatat
     4381 ggtcgtcaaa actgagactt tagaagaaaa ggcaagagaa ctattgaagg aacgtggcgt
     4441 aactgtagaa gatatcgcac aacttgtcat gtttttacaa aaagactata tttctgattt
     4501 gactttggcg gattgtactg aaagtgtcaa cagcgtgcta acaaagcgtg aagtacataa
     4561 cgccattatc actggaatcc aattagatat attagcagaa aagaaaaaat tgatgagtcc
     4621 gttgcaacat attatcgaag atgacgaagg cctttacggt atcgatgaga tcctagcctt
     4681 gtcaatcgtg aatgtttacg gtacgatcgg ctttacgaat tatggttata tcgacaaggt
     4741 gaaacctggt attttaaaag aactaaacag ccacgatggc gttcatgtcc ataccttctt
     4801 agatgatatc gtcggagcga tcgccgctgc tgctgccagt cgattagcac acaaagaacc
     4861 agaaaaaaga agtcgcctta ctcaataatt caacaaaaaa agaaagaaaa cgtctattta
     4921 gacgagttct ttcttttttg tttgtttcaa gctatttttt cctaagaaaa gaaaaacagc
     4981 aaaaaataat tctaataata caaaaaataa gataaagcta tgcatgaaaa attcttctgg
     5041 aagtacattg atgattggat cagtaacggg atcgaataac cagtcatcgt tgttgaaaaa
     5101 cgtttcatga aaaaggataa aaaagcgatc aaatcctatc atcatgaaaa aaccgaaaac
     5161 aactggcagt aacatgccga tttgaaacgg acgaatcaac cgccaaagcc gatcattctt
     5221 ttgcaaatat ttgataaaca aaatactcgg gatcaaagtg atgaaaaaca cagcataatc
     5281 cactaaaaac aaaattttca cttcataaaa gtgatgggct ccgctagcag aaactgggaa
     5341 atctggcaag ctgaggatgg ttttgaaggg attattgaga taattcatta ggtgatcaaa
     5401 atttttcagt aacgtctctt gatctaaaga agtataatcc aaaatttgca aatgtccgat
     5461 atcaaatacg tatagtggtc gaaagttgat cgtcacacca actgccagac tgatcagtgt
     5521 cagaaatagg ctgaacaatc cagcatattc catccactgc catctcgaat ttttcatatt
     5581 agaagctcca ttcatccagt gattgtttca cataggtagc tgggataggc aacccaggta
     5641 catcttcttc ttttgtaaaa cctgataaaa cgagcaaagt atctatatta ttgcgtatcc
     5701 cagcttggat atctgtttca tagttatctc caaccatgat tacttcttct ttttgcagcc
     5761 ctaatacttc aacggcttta tccataatga tggcttctgg ttttccgatg tagatcggcg
     5821 gcgtttgggt tgctgtctct acaaaagaga tgactgaccc tgctccagga agaaggccgc
     5881 gttcagtagg tatattctta tctgggttcg ttccaatgaa agtagcacct ttttgaatag
     5941 caagtgttgc tttaactact ttttcatagg tcagataatt atctaagcct accactacat
     6001 agtcaggagc ctcttcttcc catgtaaatc cggcagctaa aatcaagtca atcaatcctg
     6061 cttctccgat aacatagact tttttccctt ttccatctgc tttcataaaa tcgattgttg
     6121 ccaaagtagc tgtgtagact gtctctggag ctacatgaat gtcaaactca tctgctagac
     6181 gattagctac tgtttctggt gacttagtcg tgttattggt aacaaacaag aaaggtaatt
     6241 ttcttttttg caattcttcc acaaatcgtt taccggccgg tatgggttgt gtcccacgat
     6301 aaatcgttcc gtctaaatca attaaatatc ctttatagtc cattcatcca ctactcttcc
     6361 ctttttctga tcgtaaatgc tgtttttgtt ttttctactt gtttaggttg ttcttgtttt
     6421 tttacttttt cagaggtttt tgctgactct gtttttcgat ttttaataac cggttttttg
     6481 cgattagatg cgactggtcc ttttttctca tcgatatacg cacgattatg gttttttttc
     6541 ttttttcggc gctgagttgt cttttctttt ttaccgccga ttctctcgat gacaaaatac
     6601 gcacaaccaa aattgcaata ttcgtaaaga tagtcttcca aggaatcgat tcgctgatct
     6661 ggatgcgccc gtcgattatc cgcttggaaa aatcctttta ggcgtagttg ttcataaccc
     6721 caatctccta cgatataatc atatcgggct agtacatcac tgtaacgttc tccaagcttt
     6781 tctgcattaa atccttcacg ataatcggac acaagccgat aatgtctttc acctatcaaa
     6841 aaattactgt catctaacag tgtcactagc tcgcctttga ctttttctgc tttaggctcc
     6901 tcaacgattt cttccaaagc agctgtcagt tcttcggtga ctgttgtttc ctgttcagct
     6961 gatgattttt tcttagcaat ttctgtttta tgctcaacgt tctgctgttc attttgtttt
     7021 gcagacatgc gttcaccacc tatctttttt attatacctt gtttttattt gattgggtag
     7081 tgagctttca agtaatctcc taccacactt cggataaatt cagggaacaa aaattcattt
     7141 tttccagcaa tctcaatcgt tggaaaaaat ggaacaaaca tcaaatgatc tacggtagta
     7201 aaagtataat ttttattcag atcgattggc ttgttcttcc aaaagacttg atggttaata
     7261 gaatcatatg tgattccatt atagaccatc tgcccaaaaa tcttaccgcg aaatcccatt
     7321 cccttcatag gatagttacg taaaaagtta cgattttttt ctatttctaa aactaagcgg
     7381 atcagatcat ttccacccaa tgtgacattt aacaagtgca tcggatgcgg caacgcagtg
     7441 tgaagctgat cctgattgac ccgccctttt ggaatttcag ttaaaaacag accactgttt
     7501 aatattgctg cttccgtctt tcctcgttct ttcaccgcct caaaggctac ttggataaac
     7561 gaatgctcat tgaagatatc tagactcaga gaaaatggca aatctgctac ttctttttct
     7621 tttaataatt catgcccacg ttctagataa ctatttattt cttgttcatc ttctgtaaaa
     7681 gctgtcattg tttcggtagg gatggctttg gctgaatgct taaggatttt tttctcttca
     7741 tcgatggtta aatggacttc accgacatac tgcccgtact ttccagcggc tgctatttgg
     7801 acatcattga ttacgtgacc ttctctgaat aaatgatggg tgtgcgatcc taaaataaca
     7861 tcaattgctg gtatttcttt tgcaatttgg aaatcatcct gaatcccgag atgactcatc
     7921 aaaactaaaa catccacttg tgaacgtaaa tcttcgacta attctggcaa aatatcataa
     7981 ggattccgta tgtcccagcc attcggatta taagtaagcg gaaatggagc agtaaatgca
     8041 attagtccga ttttcgtctg gtgtttcgtt gtaatgattt tatatggttt cgtccatgcc
     8101 ggtggctgga gcgtattttt atcgaataag ttatccagga tgatatcaaa ctcagcatga
     8161 tcgtataaat gattcaactc ttctttggaa tttcctacgc cttcattgtt tcctatcgtc
     8221 gctgcatcat aaccaacttc attcatcagc aagatatttt cctgtccatt cgttgcttct
     8281 gacaatggat gccaacgatc cacaaaatca cctagatcta ctgcaatcgt tgtcgttttt
     8341 tctttctttt cgttttctcg tttacgttga tccaaaaacc ttctgatcct tggccaattt
     8401 tctaaatgag aatgcagatc attcgtatgt aaaatcacta ttttttccat gtaccctctc
     8461 gcctctcttt atctttatca taccataggc agctttttct tccattgaaa caagctctat
     8521 ttcagagttt ttcctgcctt gaagcaaatt gtataagtac aatattcgat aaatcagtct
     8581 ggctaaaaaa tcaggaacat tcatttttac agtactataa aaataaaaaa aacagacccg
     8641 aaagtctgtt gtcgtttcat tataatcaga cacatggcgg aacctgctta gtgctgcttc
     8701 cttccagacc tgacacgctt cacaagccca ccattgtcct agcctttcgg caaagaatag
     8761 tataccttat ttttcattcg ttgaccagtc tttttttaat ttcttttttc gctggcggag
     8821 ctttcggaaa aagtcagtca aaatcatccc acattcgtct tctaaaatgc caccttctac
     8881 atatgcttgg tggttaaacc gctcgtcctc caaaagattc atcaaggtac cggctgttcc
     8941 gccttttgga tcatacgcgc cgaaataaac ttctgctaca cgagacaaaa tcatcgcgcc
     9001 gctgcacata ggacaaggct ctaaggtaac aaaaagtgca gcatcttcca agcgccagct
     9061 tcccactttt tcacaggctt ccttgattgc aagcatttct gcatgtgtag tagcatcttg
     9121 cgtggtttct ctaaggttat atcctcttcc aatcactttt ccatccaata caaccactgc
     9181 tccaatcgga acttcatgaa gcatttctgc tttctttgct tctgccaatg caaggcgcat
     9241 ccatttttct ttttctactg catcaagatt tgtatttacc aattaccctt ccctgccttt
     9301 gttttttctt gttttacttt agcatatgat aaaaaggctg taactaactt ttttttctat
     9361 ggtaaaataa atacaagagg tgaacaagat gaaaatggat aaattacttg ctctttaccc
     9421 acaagcacag aaaaaaaact ctccagcatc agaagcggat atcctttctt ttgcaattga
     9481 tgatgctttt atctggatca aacaagattc attatctcaa caagaaacca gccttttgaa
     9541 agcgttattc cctgtaatca acgatcaaaa aaaacaccca tggtatcact atctttttaa
     9601 agatgcttct tgtgttcaag aaaaaagctt tcgtttgatc cagcttcacg tcgaatcaaa
     9661 aggagaattc ttaaaaaaag aatggcaaga tactattttg gaattatttc gctattctgc
     9721 tgatttcttt ttttatacag acacagatgc gctcttagtc gaacaaataa accgtgatta
     9781 tcttgatgct actgaaatca acggtatttt tctatcttta gatgctgatt ttgatacgac
     9841 aacgtctgta ttcgttggtt ctttccattc acctggcacc gacctcgtcc ctttgtttgc
     9901 tgaagaaaga aaaatttttt taacagagca aaacaatctt tatactcaca gccgtacttt
     9961 ttctatgcaa gatgtggctt tacattacta tacaaaagat actgtaaaag atagtaagtt
    10021 gatcaaaagt tacatagaac tgatcaaaca aaatgaaatg gaagtgatca ttcaagaatt
    10081 atggaatgaa ttaggcaatg tcagctctgc tgcaaaaaca ctgtttcttc atcgtaatac
    10141 tctaaaatat cgaatcgaaa aatttcaaga acagtctggt ttcaatctaa aaaaagccaa
    10201 cgatttactg ttttgctatc ttttattgct tcatgaacat aattaaagat agattaaagg
    10261 ggttgtgaca ataattttgt cacaacccct tatttcgaat gaatagtgtt caaaagctag
    10321 tttctcttac atcatttttt caacaaaatg cattagccgg tcttttacat cttcggttga
    10381 atctttgatt gaatcaccat aaacagagat tttctttttt cctgatttga tttttgctaa
    10441 cattcccatc aaagaatcca agtcactatc tgaaagacca tctacgattt ctagtaattt
    10501 ttcatttcca tcaaatttgt catagacgaa tttttttact ttcgtccgag tagtcatatg
    10561 atggatcttt tccattactt ttcccgatac aacgactgct gtcgctacac tggcagcggc
    10621 tgctacgctt aatccgattg ttacttttgt tgatgttttc ataagtatca gctccttttt
    10681 atctatctcc atcatagcat agacaaaaaa ataaacgaac taatccactc agtgaaatct
    10741 caatccttta gggaaaaagt tctttactgt tccttttact tgtttcccaa atccgacagg
    10801 tatcttatct aatactagca aaacccatcc ttgattccca tcacgttgaa atgtttctcc
    10861 tgctgtgtaa gattgccatt ccttttgagt gatcggcaag caaggtatat tctctatctt
    10921 ttttgtagca agcgctaatg catagctagg ttcaaatcga ttctttttga aatctcctaa
    10981 atgcaaacct gtacgtacga cttttaaacc atcgagagaa ggggctaact caggaacttc
    11041 ccaaagatgg tcgttgaaca ctaaaagtct tccagtagct tcataatgaa aatcatttga
    11101 aaactccgtc cagagttttt cctgctcttt tgtcatttgc actttacttt tttttcgtgt
    11161 cttcttttct ttatgcatct gattttgtcc atgaaaagtc agctttgcaa caaaatgtcc
    11221 ttcaccctga tctttatgag gccatatacg tattgttttt tccaaaccag ctacgcttcc
    11281 ccattcagaa cgtcctgaac taacggattg agtcaaagga atctcctcaa tcgttactgg
    11341 ataattttct acaagccagc taatgatctc ttcattttct tctggtgcaa acgtacaagt
    11401 cgaataaatc agttgtcctt tattttttag catttttatt gctgaagaca aaatctcttg
    11461 ctgacgtttt tgacagtata gcggagattc ttctgtccac tcttttattg catttggatc
    11521 ttttcggaac attccttctc ctgaacaagg agcatcaacc acgatccgat caaaaaagcc
    11581 cgaaaaatga gggacaagtt ctgcgggtgc atgatttgtc actattgcgt tcgaaactcc
    11641 ccatcgttct atattttctg ataaaatctt cgcacgtttc ggaaagatct cattcgtcac
    11701 taataaccct ttccccttca tttgagcagc taattgggtt gatttaccac cgggagccgc
    11761 acacaaatcc aaaacttttt caccgggttt tgctgcagca gctgttccaa cgatcattgc
    11821 acttggttct tggctatatt catacccagc ttgatggagg aaagactttc cattgacagt
    11881 gccaagaaat ccctcattgc tgtagggagc gggttgtaat tcttcgctat ggtaagtttg
    11941 gaccatgtcc aatcctgccg gttttagtgg attccaccga aatccttttt tcacgcttcc
    12001 ttgttcaagc gcagaaaaaa aatcggatgc ttcttcgcct aaaagaagac gatatttttt
    12061 gataaattgc tgtggtaacg ttgtcgcttc ctccttcatt ccaatgtttc gttagacatt
    12121 ttacaatctt ttttacgaaa aataaaggtg gctgatctac tttttcatgt tgctgcctaa
    12181 ggtgactgtt cctttaagat attttccaag tattcatccg tcacctcgtt tttatcccaa
    12241 acagcgttca tcatttcaag aaaatcttga gaagaatcat ctgagcgcaa acttcccaat
    12301 gcattaagcc ataccaattc aagcgacttt tcttctttga tcttcgtgtg acctttgaca
    12361 tatgagaaaa atggccgctt aaatagcgaa tccatgtcta aatcagattt tgcaatgatt
    12421 tcacgaatac tttcatggat agaaaaaaaa tctttcaata agtagacaat gcttaaaaca
    12481 tcatcggaac tgcattcagc taagtagttt tcaaaataaa cagtgatttc ttctaatatc
    12541 ttctctttta acccgctgat tgatttgaaa tgtgtataaa tcgggtatgt agaacaaccg
    12601 cagaactctg ctaactctct tgcagtaatt gacttcattc catattcttc cacgtatacc
    12661 atacttttga acagcaaata ttctttacta taatttgtta ctctccccat cattcttacc
    12721 tccgtctctt ttcatctacc cataaataaa taacataaat aattctaaaa atattattga
    12781 taattatcaa aacatattta gttatatcca ttaaaaatca atttttccgc cttaaaaaca
    12841 agaatatctt gacaaaatac cctaggaaat aaacaataga ataaacaagg taacaaaaaa
    12901 attaaataac gttatataaa aaagaaggcg acactctgtg agaataacac agactgaaat
    12961 gactttccac tcacagtttt tgtctaacta tatacaaaaa cagaataaaa aggaggtatt
    13021 tatgctaatt gaagcaagaa aaaaagagtt acttgttcta caaatggtca ttcaaaaaaa
    13081 taattgttct ttacaagaat tatccaatga cttacatatt ccaaaacgga caatcaaaga
    13141 actgatccga aaaataaatg tatcactcaa gcaattttta ttgattgatg aatttattta
    13201 ttctaatcat aaaggagaga tccaaaccaa agaaaccgat caaactaaaa agttagctgt
    13261 tttttctaag ttaaaattat gctacctaaa agaatccaat cgttttaact atttgatgtt
    13321 actaatcaat tatccacaaa ctgctgtacc aaaaaaatat ttacttgaac aactttatat
    13381 atcgccctcc tatctcgaaa aactgacaaa acagctcaat cattccttaa aaaaattcca
    13441 tttacggatc ctatctgtaa aaaattgctg tactcttgaa ggaaatgagt tatctatccg
    13501 tctctatctt tacttttttc tttcagatac atttggaggt aacgattggc cattttcctc
    13561 acttgaatta aatgcactaa aaatgaacag tacagatgag caaatagaaa aaatttcaaa
    13621 acaagaaaaa aatatctact tgctgttaac tttattcata ttacgaaaca aacaacaggc
    13681 atattttcca aaaacttctt ctgaaatcac tgaattgatg gacttattgc agcatacaca
    13741 tgattattca gaatacttta cttctttttc agtgagtcgc ccttgtgaag agacagaaac
    13801 agagagagcc tactttaatt attttttcta ttgttttttt ccaaatcaaa tctcttttcg
    13861 acaaaaacag gagattggtt ttttattttc cacaagcaaa aaccttattt gtcgacaagt
    13921 cggtcaattg atgacttact taactcaaca gtttcctctc aaccatttag aaaataaaat
    13981 accaatttat ttttattttc ttgtgttggc agttgttttt ttcgaactgt tgggtgaatc
    14041 cagcagtagt ttttctacac tttgctttcc agaagccacg tccaatcatt ctagttcttt
    14101 tatacaggaa aagaaattaa gagaaaaaat cgatacaatt ttttcaaaaa atggggaaag
    14161 aaaccaaatc agtcaatacc ttagtcaatt gatccaacac ttactacaaa cagaaacgaa
    14221 agagcagtta ctcatccatt tgcagataac caaacagttt acaggaaatt atcatcttca
    14281 aagccatttg cagcagttat ttcgcaaaga tgctgttaaa attaccgcat attcagaaca
    14341 tgctgatttg atcattacag atcagcatga gaaaatttca gatgaaacgg agactttcta
    14401 tctggataca acgcacaata cagtgttatt tggcaagtta ataaaagcgg ttcatgaact
    14461 gtatatgaaa aaaggttatg agaacaaaac aaagaggatg tgacaaaatt cttgtcacat
    14521 cctcttattc caaataaatg gtattcaatc agctaactct cggtattagc tcagatcaaa
    14581 gcgctgattt cacaacttct ttattttaat ctcttcggtt gccgccgaac aataagatca
    14641 agcgcaacag ctgcaggaat gtcgaaagcg ctgctgcaac ataagtcagt gctgctgcaa
    14701 ataatacgtg tcttgccatc ggtacttctt cttccgtcag tagtccgcct tcactcaaaa
    14761 tttggattgc tcgccgagac gcgttaaatt cgactggtaa tgtcaccaat tggaaaagta
    14821 atgctaaaga aaaagcgaaa atcccgatat tgattaacgt agagttccag ctaagcaata
    14881 cacctaccat aatcaagggg aacgaaatca tcgaaccaaa gttagctgct ggaacaagtg
    14941 ctgcacgaat cttcaatggg acataacccg aagcatcttg taccgcatgt ccgcattcat
    15001 gagctgctac acctatcgca gcaactgaag tagactgtgc agttgcttga gacaaactca
    15061 acatcttatt acctgaattg tagttatctg tgagatctcc ggcaatttgt tgaacaccga
    15121 cattatttat ctgttgactt tgtaatatat attgtgctgc ttgtgtacca gtcacattgt
    15181 ttttgcttct cacttgatcg tattttcgga aagtactatt gacgtacgct gacgcaattc
    15241 cggaaataac taatccaaga atgaccaaaa gaaatgtcgg atctaaaaaa ccatacattc
    15301 cataaccata cattttcgtt tcactccttt tcgtttactt tattttatca ttagtagtag
    15361 gaaaagaatg tgaaaagacc ttgaatattc tttaattctt tttcacaaat tccgatttca
    15421 gtttcatcgg tccaaatcct tctaccttgc aatcgatatt atgatcgcct tctactaaac
    15481 ggatgttctt gacttttgtt ccttgtttaa tggcacctga cgcacctttt actttcaagt
    15541 ctttaatcac tgtcacgcta tcgccctcta acagacggtt tccatttgca tctaatacca
    15601 ctaatccgtc ttctatgaca gtttcttgtt cttctttgtt ccattcgttc ccgcattctg
    15661 ggcaaatcaa caagcctcga tcttcatatg tgtactctga gccacattct gggcaatttg
    15721 gtaatgtcat cttctttctc ctttaaaatc tttagtgagc actattgtaa cggaaaaaga
    15781 agatcttgaa aagatacatt tgtgattttt actacaaaag gcacgttttc gaacgaagta
    15841 tcattctgtt tttctgctct tatacgattg gcatatatct tggatctctt tatagatttc
    15901 ctctacttct gctaaatctt ttgcattcgc accacttgct aatggatggc ctcctccatg
    15961 atggcgttta gccaattcat tgatgatcgg ccccttagaa cgtaaacgta cgcggtaata
    16021 ccctgaaggc tgctgaacaa aaatccccca agccagcact tcgtcaatca ctccaggaag
    16081 tgaaacgatc gaagctgtct ctgaatcatc gattccatat ttatcaagaa tttcttgcgg
    16141 aaggattatt cttcctgcac cattttcatc cacctggata ttttgataaa gataaccgga
    16201 aagtttagcg actttcatcg gcatttgttc aatctctctg ttcaaagcac ttgcatcaaa
    16261 tggataattt ctcaatatcg aagcaatctc taatgtacgg gacgtcgtgg agggatacaa
    16321 aaagcgccct gtatctccga cgattcctgc ataaagtaac cgtgcagcct taacgttcat
    16381 ctttaaagat tcaggaaaca tcaaagcaaa ttctgcaatc atctcactac agctacttgc
    16441 ttttgtattt acccacacaa gatctccata agggtcatca ttgggatgat ggtcgatttt
    16501 gatcagtttt tctgccaact gatatctttg atcactgatt ctgggtgaat tagctgtatc
    16561 agtcacgatg actagcgctc cttggtagaa gacatcttca acttcttgca tttctgctaa
    16621 aaagcgaagc ccttcaatcg tttcacctac ttgacgtatt tctttttctg ggaaactttc
    16681 ccgtaatagt tcggctaatc ctacttgtga tcctaatgca tcaggatcag ggcgcaaatg
    16741 cctgtggata atgattttat cgtatttttt tattgtatct actatttctt gctgtactgt
    16801 catttcactc ctgctttcta agaacgttcc attacttgac aaacaacgat tgctttggaa
    16861 acgatggtgt tttcaataaa cacttcgata tccagtttag cggaacgtct gccgatttcc
    16921 aatacacgtg gacgaatgtc taattcgctt tccaattgga tcagccgcaa ataatgtagg
    16981 ttcacttgtt caatcaagat atttcgtttt tggttcatca ccattgtttt ttgtgcgaca
    17041 tcagaaatga tttcgctaag tacgccaaaa gaaatggttc caacgccatt gaccatttgt
    17101 ggtgctacta cgaatttgaa cttagggttt tccaaacgat ttccttcacg gtcagtatca
    17161 atcgtatgga tatttccaga tatctgatcc gaaatcgtat ctgcaatctg cggttgtcgc
    17221 tggacaagct gcatcgcttt cattacatct tgccgggtaa tcaagccttc caatgtcaag
    17281 tcatcggaaa cgaccggcat gacttctagc ccatcccaga tcatttgatg gctaacagag
    17341 gcaatactca ttgttttctt tacactgatt ggttctttcg tcatcacccg atcgatgatc
    17401 tggtttggcg atttccctaa aacatctttt gccgtaacaa tccccatcaa acgatgatgg
    17461 cggttgacga ctggaaaacg cgaatgctgc gtttcttctg acaaacgctg ataatcagaa
    17521 atcgtatctg tctgctgcaa ataatgagtc ttttcagctg gcatatagat atcactgaca
    17581 agcatgatat cttttttgat caattggtcg cttaacgctc ggttgatcat ggttgcgact
    17641 gtaaatgtat cataagaggt gcgtaagatc ggcatttcaa gatcatcagc tagctttgca
    17701 atctcagcag tcgtattgaa tcctcctgtg atcaatactg cggcgccatc ttccaatgct
    17761 aatttttgga cttccgcccg gttgccgacg atcattagtg atcctggcgt aatatagcgt
    17821 tccatggcat gtttagtcat cgcgccgatg acgaatttat tcaagacctt gtctaatcct
    17881 gccgccccgc ctaaaacgtc cccttcaatg atcctgacga cctcgccaaa tgtcaaacgt
    17941 tcaatatgtt tttttaattt gcgttcgata cggatggtcc ctactcgttg aattgttgaa
    18001 actagtccga cattttctgc atctttgatt gcacggtacg ctgttccttc actgacattc
    18061 aattcttttg cgatgcttct tacagaaatt cgattgccaa caggtaaact ttcaatatat
    18121 tctaaaatca aatcatgttt tgtcgccatt ttctgcctac acttccagcg tttccgagac
    18181 gctcattatt tttccttcac tatcttccag taactctaca aaagattcgg gatcttgttc
    18241 aatgacaggg aaagtattgt aatggattgg aactactttc ttggcacgaa ttgtttgcgc
    18301 agcctttgct gcatcttctg gccccatcgt atagttatcg ccaatcggca agaaagcgac
    18361 atcaatatcg aattcttctg caagcaatcc tagatcactg aaataagcag tatcccctgc
    18421 atgataaatc gtttttcctt ctgcttgaat aataattccg ctaggctcgc ccatatactg
    18481 aacgataccg tctacttcat agcttgaact atgctgtgca tgtacgaatt ttaccacgcc
    18541 gaatggaaac gcatgttttc caccgatatt cataccgaat gaatctactc ctctgctgtt
    18601 cgcataatca gcaatctcaa caatactgat aattgtggct tgattttctt ttgcaatagg
    18661 aatcatatct cctatgtggt cactatgacc atgagtaatc agcaaccaat ctgtctttac
    18721 ttcttctgca gttaagtctg tcaatggatt tcctgtaata aacgggtcaa tcatcacgcg
    18781 ttggccattt tccatttcaa ttgttaaaca ggaatgtcca tgatacgtaa ttttcatatt
    18841 ttacacctcg cgttttctct gtatcttttt tattaaaaac gatttcatca cttgcacatc
    18901 aatgaaaaaa tatagctgct aaatatattt tcattggatt gatagtacag tatgtacaaa
    18961 gcaattgtaa cagataatac aggatgcacc aagtaccaag cagatataat ctcaagtttg
    19021 aggtctctgc tctgcttagc ttctcttctc gtcccttaac taattaaatc agcgtaaata
    19081 ggatggacga tcttttctaa atcggctact tttatccgaa ggcttctccc gatttcaccg
    19141 cttgacacaa ttacttcttc tttttctttt aaagaactgt ctgctaaaaa acaataatca
    19201 ggatgtgcga gttttattcc aataggggtg tttgctccgt ggggatatcc tgtatgcatt
    19261 aatacaaaat ccattccagg aaaaccaact ttacgatctt tcgtcacttg tctcgttttt
    19321 ttgtaatcta agtgatcgtt caaaggaatc aatgcgatga tcaccccagt tttgtttcct
    19381 ttcactacga gagttttaca tatcaaagct ggatcaatcc ctcttttttg tagttcttct
    19441 gtgaaagatt cttctaccac atgactaaaa tcaagtggtt gatagctgat cttttgttct
    19501 tctaaataac gttcgacttt tgtttgactc atcattgatt cactcccttt attctatcgt
    19561 ttattataaa gcgagggatg ctgtattcaa aaatta
//
LOCUS       DAZKLD010000044        19532 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.044, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000044 DAZKLD010000000
VERSION     DAZKLD010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19532
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.044"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            286..1014
                     /gene="purC"
                     /locus_tag="ACG8KR_001271"
     CDS             286..1014
                     /gene="purC"
                     /locus_tag="ACG8KR_001271"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HFQ0200650.1"
                     /translation="MEKKELLYEGKAKKIYLTSDENLIWIEYLDQATALNGARKDQIS
                     GKGELNNQITSAIFRYLTQRGIKNHFIEQLSKTEQLAKKVQIIPLEVVIRNIAAGSFS
                     KRLGVPEGKEFIKPIIEFYYKDDQLDDPFINEEHIYFLQLAKPEEISELKAQALAINE
                     ILTELFAKVGLVLVDFKLEFGRSNGQILLADEVTPDTCRLWDERTNEHMDKDVYRRDL
                     GDLIPVYQEVYNRLSELTEEKERV"
     gene            1014..1268
                     /gene="purS"
                     /locus_tag="ACG8KR_001272"
     CDS             1014..1268
                     /gene="purS"
                     /locus_tag="ACG8KR_001272"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HFQ0200651.1"
                     /translation="MYFVKVYVTYKDSVLDPQGEAVKKAVHRMGYETIEDIRIGKYFE
                     IQLADAEEPIDTVIETICDKLLANVNMETYRYEIKKMEEV"
     gene            1270..1941
                     /gene="purQ"
                     /locus_tag="ACG8KR_001273"
     CDS             1270..1941
                     /gene="purQ"
                     /locus_tag="ACG8KR_001273"
                     /EC_number="3.5.1.2"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HFQ0200652.1"
                     /translation="MKFAVIVFPGSNCDADMLWAVREIMGADAEFVRHDESSLEGFDG
                     VLLPGGFSYGDYLRCGAIARFSTIMEEVIRFAEEGKPVFGTCNGFQILTEAGLLPGVL
                     RRNESLHFICKTVPLKVNPKTAFTSEYEADEIIYLPVAHGEGNYFCDEETLQELKKNN
                     QIAFTYNDNINGSVENIAGITNKAGNVLGMMPHPERAVEDLLGSEDGKRFFASILKNF
                     GKVTV"
     gene            1942..4164
                     /gene="purL"
                     /locus_tag="ACG8KR_001274"
     CDS             1942..4164
                     /gene="purL"
                     /locus_tag="ACG8KR_001274"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HFQ0200653.1"
                     /translation="MKTLKEPTAEEIKKKRIYAEWGLTDEEYRMIEEDILGRMPNYTE
                     TGLFSVMWSEHCSYKNSKPVLRKFPTSGPHVLQGPGEGAGIVDIGDGLAVVFKAESHN
                     HPSAVEPYEGAATGVGGIIRDIFSMGARPIALLDSLRFGELDNPRTKYLLEEVVAGIG
                     GYGNCIGIPTVGGEIAFDPCYEGNPLVNAMCVGLIEHKHIQKGQAAGVGNSIMYVGAK
                     TGRDGIHGATFASEEFNQEEEQQRSAVQVGDPFMEKLLLEACLELILHHSDSLIGIQD
                     MGAAGLVSSSAEMASKAGSGLILTLDEVPQRETGMTPYEMMLSESQERMLICVKSGEE
                     EKIQELFQKYDLDAVTIGKVTDDGQYRLYHQGEEVANLPVDALAEDAPVYHKEMKEPA
                     RIVEFQQLAPYQPVIEEPGQVLLDLLQQPTIASKRSIYETYDSQVQTNTVVRPGSDAA
                     VMRVRGTNKALAMTTDCNARYLYLDPKIGGQIAVAEAARNIIASGGKPLAITDCLNYG
                     SPDKPEVFWELSTSADGIAAACETLGTPVISGNVSLYNETDGQAIYPTPMIGMVGLIE
                     DHKHITTQEFKKSGDLIYILGKTFADFDGSELQKMQLGRIEGVIRNFDLSIEKRNQEL
                     VLTAIQNGLIESAHDCSEGGLAIALAESAFKHQLGISVQFELSSAQLFAETQSRFVLT
                     VAPENKTRFEEMMGDAAVLAGKVTDEAIIEISATDGQIKIETAVARKCWEDAIVCLMK
                     "
     gene            4149..5588
                     /gene="purF"
                     /locus_tag="ACG8KR_001275"
     CDS             4149..5588
                     /gene="purF"
                     /locus_tag="ACG8KR_001275"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HFQ0200654.1"
                     /translation="MSYEVKSLNEECGIFGVWGHPDAASVAYFGLHSLQHRGQEGAGI
                     VSNDRGKLKGHRDLGLLSEVFKDQRKLQQLTGEAAIGHVRYATAGNGSVDNIQPFLFK
                     FHDQSIGLAHNGNLINARSLRKELEENGAIFHSNSDTEILMHLIRRSEETDFLNKLKS
                     ALNEVKGGFAYLLMTETAMIAALDPNGFRPLAIGQMKNGAYVIASETCALEVIGARFI
                     RDVQPGEIVIINDEGIQIDCFTKDTQLSICSMEYIYFARPDSNIAGINVHTARKNMGR
                     NLAKESPVKADMVVGVPNSSLSAASGYAEASGIPYEIGLVKNQYIARTFIQPTPELRE
                     QGVRMKLSAVRGVVEGKKVIMVDDSIVRGTTSRRIVHLLKEAGAKEVHVRIASPPLKY
                     PCFYGIDIQTRKELIAANHTIDEIKECIGADSLGFLSEEGLIESIGLEKDAPYSGLCM
                     AYFNGDYPTPLYDYEEKYQESLKEKVSFF"
     gene            5611..6663
                     /gene="purM"
                     /locus_tag="ACG8KR_001276"
     CDS             5611..6663
                     /gene="purM"
                     /locus_tag="ACG8KR_001276"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HFQ0200655.1"
                     /translation="MNSMANAYAKAGVDVEAGYEVVERIKKHVKKTERLGMMGAIGGF
                     GGCFDLSVFQLKEPVLVSGTDGVGTKLAVAIEADKHDTIGIDCVAMCVNDILAQGAEP
                     LYFLDYLATGKNDPLRLENVVKGVADGCVQGNMALIGGETAEMPGMYSGNDYDLAGFA
                     VGIAEKSQLLTGETINPGDVLLGIASSGIHSNGYSLVRKIFFEQQQFSLSSKLPELKK
                     TLGEELLEPTKIYVSSLLPLIKEKKIKGAAHITGGGFVENIPRMLPESMAAQIKLGSW
                     PILPIFGCLQKYGEIPEMEMYEIFNMGIGMVLAVEESKVKEIVHEIEQAGEKVYEIGK
                     VIAKEKHRVVFIGEER"
     gene            6660..7238
                     /gene="purN"
                     /locus_tag="ACG8KR_001277"
     CDS             6660..7238
                     /gene="purN"
                     /locus_tag="ACG8KR_001277"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HFQ0200656.1"
                     /translation="MRIAVFASGNGSNFQALADYLSKKGLESSIDWLFCDQPEAYVLK
                     RATALSVPADCFSPKEFDSKKEYEEAILHKLKEKKIDLIVLAGYMRIIGPVLLKNYDK
                     RIINIHPSLLPAFPGLHGIRDAFEAGVKETGVTIHYIDQGVDTGPIIRQEKVRIEQED
                     TFDSLEEKIHRVEHRIYPEVISEIIENGGYLK"
     gene            7235..8770
                     /gene="purH"
                     /locus_tag="ACG8KR_001278"
     CDS             7235..8770
                     /gene="purH"
                     /locus_tag="ACG8KR_001278"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HFQ0200657.1"
                     /translation="MTRALISVSDKTGIVAFAQGLIEQGVEIISTGGTKHALDKAGIK
                     TLSIEEVTGFPEMMDGRVKTLHPKIHGGLLGRRDLPSHMEAMETENIQPIDFVCVNLY
                     PFKETIMKPDVEIAEAIENIDIGGPSMLRSAAKNHEFVTSVVDPSDYEEVLSELRQYG
                     HTSLSFRQKLAAKVFRHTAAYDALIAQYLTDWVEEKEPEKLTLTYERKQTLRYGENSH
                     QAAAFYQSALLEKYSIAAAKQLHGKELSYNNIRDADAALRIMKEYDEPTVVALKHMNP
                     CGIGTASTILEAWEAAYEADPVSIFGGIIVLNREVDLPTAEQMHKLFLEIIIAPSYTQ
                     EALELLQKKKNIRLLTVDFAQEENQNREETVSVLGGLLVQDQDLAIENEEEWKVVTKR
                     KPTPAEAKALVFAWRAVKHVKSNAIVLANERQTVGIGAGQMNRVGSVKIAIEQAQEKM
                     EGAVLASDAYFPMDDSVEYAAKHGIKAIIQPGGSIKDQDSIDMANKYDLAMVFTDVRH
                     FRH"
     gene            8786..10060
                     /gene="purD"
                     /locus_tag="ACG8KR_001279"
     CDS             8786..10060
                     /gene="purD"
                     /locus_tag="ACG8KR_001279"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HFQ0200658.1"
                     /translation="MNILVIGAGGREHAISKKLLASPKVDTVYCAPGNPGMTKDGINC
                     VAISERDHSALIHFVKKEAIDWSIVGPEVPLLNGIVDDFMAEGLKIFGPTKAAALIEG
                     SKSFAKQLMDTYQIPTAKSRSFECYEKAAAYIKENGAPIVVKADGLAAGKGVVVATTV
                     KEALDAAEQMLNQHRFGDSSKKIVVEEFLAGEEFSLLAFVRNTKVYPMVISQDHKRAF
                     DNDKGPNTGGMGAYSPVPQISKKMIEKAVEEVLKPAALGMKELGRSFTGVLYAGLIAT
                     KEGPKVIEFNARFGDPETQVVLSRLKSDFAQVIDDLLENRTVELKWQQEGYNVGVVVA
                     AAGYPEEYETGMVLPDFLEEELSVYYAGVSSNNGKLVGAGGRLYLVEAYGETIKEAQK
                     KIYTALMDKTTDGTFYRTDIGEKAANSELIVK"
     gene            10182..11096
                     /locus_tag="ACG8KR_001280"
     CDS             10182..11096
                     /locus_tag="ACG8KR_001280"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HFQ0200659.1"
                     /translation="MKKIQLGTSDQKAAAVILGCMRINNAEDPVKVIETAYENGIDFF
                     DHADIYGNGECEEIFADALAKTSIKREDLFIQTKCGIVPGVMFDFSKKHIIESVEKSL
                     TRLKMDYVDALLLHRPDTLVEPEEVAEAFDELERSGKVRYFGVSNQKPMQIELLKKYV
                     RQPLVANQLQFGLKHTGMIDQGIHVNMTDEASIDHDGSVLDYSRLHDMTIQAWSPYQY
                     GFFEGVFIGNEKFPELNQTLEELAAKYQTTPTGLASAWILRHPANMQVIAGTMNVGRI
                     KEIAQASEINMSREDWYELYRAAGNILP"
     gene            complement(11229..12794)
                     /locus_tag="ACG8KR_001281"
     CDS             complement(11229..12794)
                     /locus_tag="ACG8KR_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0200660.1"
                     /translation="MYKVFIIEDEYLIRNNLREQITVLGSYYPITYVGEAGDGEMALS
                     SIIDLQPDIILTDIQMPFMDGLTFSKEARKLLPWTRLIFISGFDDFDYMKGAIQVQAD
                     DYLLKPIKSSELKQAIEKAVTALDLQQKETHTSSDLVFELKKNHFLNGLFKGSLTISE
                     TLKLAESFNRSLAGKKMTVLLAANQVSTDFEDYAHLSDYLKFLFRENQQVIFSAVSSR
                     FIKFLIVDSLEENVIETSYQVAQTLVHELEKGQKNNFSVAIGPVAERISELPASFEIT
                     QMILETASSLKEQIISYEDLIQEAKLPVDSVFYFDLSLDLSSLSKNEVSKYIEHLTKP
                     QGTPTRTRLYRLFVLTKLLQHVCLKEKKIPQEMVHCTSTSQRLAISADDSLYRSTVYM
                     FISFLTALPSQPSRNKYQPILNHALDFIKENFTDPDMSLTWVAQQVALSPSHFSTIFS
                     QSFNQTFIDYLTEQRIELAKKLLQQSDYRISDIAFEIGYNDPNYFSYLFKKKQGISPK
                     EYRTQLFLKNKNN"
     gene            complement(12787..14238)
                     /locus_tag="ACG8KR_001282"
     CDS             complement(12787..14238)
                     /locus_tag="ACG8KR_001282"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFQ0200661.1"
                     /translation="MTKNLSIKKIFAQANKAMLLFAVIPLLLSILLYSQQLLIYQQTI
                     ANIQKADAIALKVDQKVLESMWDLISGQVTVDEFNEENILEELRNDIRESKNNTNTLK
                     EISTLDVSLRTIDTLEDYQNELINNILAKDFMDTNEEIINQVDSVTQLLSDILQDFVR
                     VEIDLASKKNTTLVRSLILLTIVELGVLSTIFFYAKKNRRFLERKVQEPINQLIEMSE
                     QLSSGNLGFRLTLPETLELQTLTASMNKMAEDLNQSLRENALKQYHLAQSEVRVLQAQ
                     ITPHFIYNSLDAIISLIEQDCFEEALTMTHALSGFFRTSLSKGQDWIPLEKEISHIND
                     YLVILKIRYGHMLDYKIDIPETLFPVSILKMILQPLIENAVYHGTKYVRHKGFIRITT
                     CKIDEMIQFKVIDNGIGIPAEKLAEIRKELAKGIDSDFSIGYGLYNVNKRLLLYYGND
                     QKPITIDSKYREGTTVTLMIPLSPKTIGGNKHV"
     gene            complement(14252..15268)
                     /locus_tag="ACG8KR_001283"
     CDS             complement(14252..15268)
                     /locus_tag="ACG8KR_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFQ0200662.1"
                     /translation="MKRVLFFLALVVIAYQMMGCTPNTKEAPDPLVVGFSQSGTESSW
                     RKKHTESIITALEKEDYQVLYRNGYMNQERQIQDIRTFIAYKVDMIIFTPLQESGWDS
                     VLKEAKRAGIPVILVDRHIQTNDPELFVTHIGPSFKAEGNRAGLFVSNHFTNSKKQEI
                     RILELAGSENSTPTKLRSEGFLESINRKPTLKKDGTIDHKPPMTIVHRIVGDFIRMKG
                     KDQMKRYLETNDLSEIDVLYSHSDEMTHGALAAIKETSIKPGEDLIIVTIDGQEDMIE
                     KLRSGIVNCVVECKPDVGWYVANTVTRYFGNIQTGKTLPKDIFISETVFSQQNIANIP
                     TRNY"
     gene            15488..16576
                     /gene="chvE"
                     /locus_tag="ACG8KR_001284"
     CDS             15488..16576
                     /gene="chvE"
                     /locus_tag="ACG8KR_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     substrate-binding protein"
                     /protein_id="HFQ0200663.1"
                     /translation="MKNKFRKIVGTIAILTASILLSACGNSGSADDSTGYVGIAMPTK
                     SAERWIADGNNMVSELEKLGYKTDLQYGEDKVENQVAQIENMITKGVDTLVIASIDGS
                     ALTDVLAKAKEADIKVIAYDRLLMNSENVDYYATFDNFGVGVSQAAYIEEHLGLKEGK
                     GPFTIELFGGSPDDNNALINYNGVMSVLQPYMDNGQLVVPSGQTSFSQIATLRWDGST
                     AQARMDNLLSANYTDQTLDAVLSPYDPISLGIISSLKGVGYGSESKPLPVITGQDATV
                     AGVKSIIAGEQTQTIFKDTRILAKNTIEMIKAISDGEEVPVNDTETYDNGVKTVPTYL
                     ANTVSVDKDNYQAELIDTDYYKESDLKN"
     gene            16630..18156
                     /gene="mmsA"
                     /locus_tag="ACG8KR_001285"
     CDS             16630..18156
                     /gene="mmsA"
                     /locus_tag="ACG8KR_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     ATP-binding protein"
                     /protein_id="HFQ0200664.1"
                     /translation="MADCILKMADITKKFSGIRALDCVNLMIERGEIHALCGENGAGK
                     STLMNVLSGLYPYGSYDGEIFYDGKLCQFKDLKDSEEKGIVIIHQELALSPYLSIKEN
                     IFLGNEQAERGVIDWELTEKKTYELLNKVGLSIDPNLLVSQIGVGQQQLVEIAKALSK
                     SVRLLILDEPTAALNEEESANLLRLIRELKEQGVTSIIISHKLNEIVDIADRITILRD
                     GQTIETLENEMISEEHIIRGMVGRDLVDRYPERKPDIGEVYFKVEDWTVYHPLDHERI
                     INDHINLHLKKGEIVGIAGLMGAGRTEFAMSLFGHSYGSKISGRILKEGAEISVKTVP
                     SAIANGLAYVSEDRKSLGLNLLMDIRENVTLSSLDKISQKGVLDKEKEVTEAENFRKK
                     MRIKTNSIHQIVSSLSGGNQQKVVLSKWLMTQPDILFLDEPTRGIDVGAKYEIYTIIE
                     EMAANGKAVCLISSELPEILGMCDRIYTMNEGRITGEVSREDADQEVLMKLMTKEEVG
                     "
     gene            18158..19363
                     /gene="mmsB"
                     /locus_tag="ACG8KR_001286"
     CDS             18158..19363
                     /gene="mmsB"
                     /locus_tag="ACG8KR_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     permease"
                     /protein_id="HFQ0200665.1"
                     /translation="MEQAKNLTDKKEKKLNLKSRAADLFGRYSMVIILAGLLIAFQLM
                     TDGIFWRPLNITNLVLQNSHILVLAAGMLLVVLLGHVDLSVGSVMAFVGAIAGMMMVN
                     RQMNPWMTVIACLAVGGLIGAWQGFWVAYAGIPAFIVTLAGLLMFRGLTQVVLGGQSL
                     APFPAVFQKMSTGYLPDLSEGSSLHFLTLILGMIITIVLVVGQWRTRNRRKNHLFEVE
                     SPTAFLVKAVFTAVVVLGITYIFASYQGYPIILIILGLIVAAYGFLTNKTVAGRQIYA
                     TGGNRKAAELSGIKTKRITFWVFVNMGVMAALAGLVLAARLNAATPQAGTSMELDAMA
                     AVYFGGASTSGGIGTIMGAIVGGLVMGVLNNGMSILGVGVDWQQAIKGLILLLAVVLD
                     LYNKKKRTT"
BASE COUNT         6240 a         3321 c         4461 g         5510 t
ORIGIN      
        1 aagctgacct tgttttattg catcttccat cggcaagtgc ctcctcgaac ccattaacca
       61 aatttttagt taattctaat ataccatcat taagtataaa tgtaaacgct tgttgatggt
      121 ataagatttt cgtttctgat aaaacacgaa cattaaaaga atctaatgcc ttaaatgtac
      181 atatttttgt tgatttataa aaagatgatt gctaaaattt aaagtgaaaa aacgaacaaa
      241 aaacgatgta tttgctttta atgtaaataa aaaggagaga cgtacatgga gaagaaagaa
      301 cttttgtatg aaggaaaagc caaaaaaata tatcttactt ctgatgagaa cttgatttgg
      361 atagaatatt tagatcaagc aactgcgttg aatggcgcga ggaaagacca gataagtgga
      421 aaaggagaat tgaataatca aatcacaagt gcaattttcc gttatctgac tcaaagagga
      481 ataaaaaatc attttattga acaattgtct aaaaccgaac aattagcaaa aaaagtacaa
      541 attatccctc tggaagtggt tatccgaaat attgcagcag gaagtttctc aaaacgtcta
      601 ggtgttcccg aaggtaaaga attcatcaaa ccaatcattg aattctacta taaggatgac
      661 cagttggatg atccatttat caatgaagag catatctatt tcttacaact tgcaaagccg
      721 gaagaaatca gtgagctgaa agcccaagcg ttggctatta atgaaatttt gaccgaatta
      781 tttgctaagg tcggtttggt tttagtagat ttcaagctag aatttggccg gtcgaatgga
      841 caaattttgc ttgcggatga agtaacaccg gatacttgcc gattatggga tgaacgtacg
      901 aatgaacata tggacaaaga cgtttaccgc agagatctag gtgatttgat cccagtctat
      961 caagaagtat ataaccgtct atcggaacta actgaagaaa aggagcgagt atgatgtatt
     1021 tcgtaaaagt atatgtaacc tataaagatt cagtgttaga tcctcaaggg gaggcagtaa
     1081 aaaaagcagt ccatcgtatg ggatatgaaa caatcgaaga tatccgaata ggaaaatact
     1141 ttgagataca acttgctgat gcagaagaac caatcgatac agtgattgaa acgatttgtg
     1201 acaagctgct tgcaaatgta aatatggaaa cataccgata tgaaatcaaa aaaatggagg
     1261 aagtataaca tgaaatttgc ggtcatcgtt tttccaggtt caaattgtga cgcagatatg
     1321 ttatgggcag ttcgcgagat catgggcgct gatgccgaat ttgtacggca tgatgagtcc
     1381 tctttagaag gatttgatgg ggttttactg ccgggaggat tctcctatgg tgactatttg
     1441 agatgcggtg cgattgctcg gttttctaca atcatggaag aagtgattcg ttttgctgaa
     1501 gaagggaaac cagtattcgg tacatgcaat ggattccaga tattgacaga agcaggactt
     1561 ttgccaggtg tactgagacg aaatgagtct ttgcatttta tttgcaaaac tgtaccattg
     1621 aaagtcaacc ccaagacggc ctttacttca gagtatgaag ccgacgaaat aatctatctt
     1681 ccagttgctc atggggaagg aaattatttc tgtgacgaag aaacacttca agaactaaaa
     1741 aaaaataatc agattgcctt tacttataat gacaacatca acggcagcgt ggaaaacatt
     1801 gcagggatta cgaacaaagc agggaatgtg ttaggaatga tgcctcatcc agaacgtgca
     1861 gtagaagact tgctaggatc agaagatggg aaacggttct ttgcttccat tttaaaaaac
     1921 ttcggaaagg tgaccgtata gatgaaaaca ttgaaagaac cgacagctga agagatcaag
     1981 aaaaaaagaa tctatgccga atggggattg actgatgaag aatatcgaat gatcgaagaa
     2041 gatatcttag gaagaatgcc taattatacg gaaactggac tgttttctgt tatgtggagc
     2101 gaacattgtt cctataaaaa ttctaaaccg gttctacgca aatttcctac tagtggacct
     2161 catgtgttgc aaggccctgg agaaggcgca ggcatcgtcg acatcggtga tggactagca
     2221 gttgtcttca aagcagaaag tcacaatcat ccttcagccg tggagcctta tgaaggagct
     2281 gcgacaggag ttggcggaat catcagagat attttcagca tgggggctcg tccaattgcg
     2341 ttgctagatt cattgaggtt cggcgaatta gataatccaa gaacgaaata tttactagaa
     2401 gaagtagttg caggtatcgg cggatacggc aattgtatcg gcataccgac tgtaggcggc
     2461 gaaattgcat ttgatccttg ttatgaagga aacccattag tcaatgcaat gtgcgtaggt
     2521 ttgattgaac acaaacatat ccagaaaggg caagccgctg gcgtcggtaa ttcgatcatg
     2581 tatgtaggtg caaaaacagg tcgcgacggt atccacggag caacttttgc ctcagaagaa
     2641 tttaatcaag aagaagaaca gcaacgttct gctgtacaag taggcgatcc ttttatggaa
     2701 aagctgctgc ttgaagcgtg tttggaatta atcttgcatc attcggattc tctaataggt
     2761 atacaggata tgggagcagc aggacttgtt tcatctagtg cggaaatggc ttcaaaagct
     2821 ggctcaggac ttatattgac attagatgaa gtcccacaaa gagaaactgg gatgacacct
     2881 tatgaaatga tgttatcgga atcacaagaa cggatgttga tttgtgttaa atcaggagag
     2941 gaagaaaaaa tccaagaact atttcagaag tatgatctag atgcagtcac tatcggtaaa
     3001 gtaacagacg acgggcaata tcggctatat catcaaggag aagaagtagc aaatctgcca
     3061 gtcgatgcat tagctgaaga tgcaccagtt tatcataaag aaatgaaaga accagctcga
     3121 atcgtagaat ttcagcagtt agctccttat cagccagtta tcgaagaacc tggacaagta
     3181 ttgcttgatc tgctgcagca accgacgatt gcttcaaaac gttcaatcta cgaaacttat
     3241 gattcacaag tacaaaccaa tacagttgtc cgtccaggaa gcgatgctgc tgtgatgagg
     3301 gttagaggga cgaataaagc gttagctatg accacagact gcaacgcgcg ctatctgtat
     3361 ttggatccaa aaatcggagg acaaatagca gtggcagaag ctgctagaaa tattattgct
     3421 agcggtggta agcccttggc gatcaccgac tgcctgaact atggttctcc tgataaacca
     3481 gaagtttttt gggaattatc gacttcagct gacggaattg ctgctgcgtg tgaaacgtta
     3541 ggaactcctg ttatttcagg aaatgtctct ttatataatg aaacggatgg tcaagcaatc
     3601 tatccaacac caatgattgg tatggtcgga ttgattgagg atcataaaca tatcactact
     3661 caggaattca aaaaatcagg ggatctaatc tatatattag gtaagacgtt tgcagatttt
     3721 gatggcagtg aattacaaaa gatgcagctt ggaagaatcg aaggtgtgat ccgaaacttt
     3781 gatctatcca ttgaaaagag aaaccaagag ttagtattga cagccatcca aaacggattg
     3841 attgaaagtg ctcatgactg ttcagaaggt ggcttggcaa ttgcactggc agaatcagct
     3901 ttcaaacatc agctagggat atctgtacag tttgaattgt cctctgctca attatttgca
     3961 gaaactcaat caagatttgt gttgacagtc gcaccggaaa ataagacacg attcgaagaa
     4021 atgatgggag atgctgctgt tttagcaggt aaggtgacgg atgaagcgat aatcgaaatt
     4081 tcagcaactg acggtcagat caaaatagaa actgcagtag caagaaagtg ctgggaggat
     4141 gcaatcgtat gtcttatgaa gtgaagagtt taaatgaaga atgcgggatc ttcggtgttt
     4201 ggggtcatcc agacgccgct agtgtggcat acttcgggct ccatagtctt cagcatcgag
     4261 gacaagaagg tgcaggtatc gtgtccaatg atcgaggaaa gcttaagggg catcgagatt
     4321 tggggctgct ttctgaggta ttcaaagatc aaagaaaatt gcagcagtta acaggagaag
     4381 ctgcaatcgg acatgtacgt tatgcaacag caggcaatgg aagtgtggac aatattcagc
     4441 cttttctatt caaatttcat gatcagtcga tcggcttagc gcataatggt aacttgatca
     4501 atgctcgttc ccttagaaaa gagttagagg aaaatggggc gatcttccat tctaactccg
     4561 atacggagat attgatgcat ttgatccgac gaagtgaaga aacagatttt ttaaataaat
     4621 taaaaagtgc tttgaatgaa gtcaaaggtg gctttgccta tttattaatg acagaaacgg
     4681 caatgattgc cgctttggac ccaaatggtt ttcgtccatt agcaatcgga caaatgaaaa
     4741 acggagcata cgtgattgcc tcggaaactt gtgcattaga agttattggg gcacgtttta
     4801 taagagatgt ccagcctggt gagatagtga tcatcaatga cgaaggaatc cagattgatt
     4861 gctttacgaa ggacacgcag ctttctattt gttcgatgga atatatctat tttgccagac
     4921 cagattcaaa catcgcaggg atcaatgtgc atactgcacg gaaaaatatg ggacggaatt
     4981 tagcgaaaga atcgccagta aaagcagata tggtcgttgg cgtacctaat tcttctttgt
     5041 cagccgcaag cggttatgcg gaagctagtg gaatccctta tgaaatcgga ttagtaaaaa
     5101 atcaatacat tgcccgaaca tttatccagc caacgcctga gctaagagag caaggtgttc
     5161 gaatgaaatt atctgctgta cgaggagtag tggaaggtaa aaaggtgatt atggtcgatg
     5221 attcaattgt tcggggtacg acaagcagac ggatcgtcca tcttttgaaa gaagcagggg
     5281 caaaagaagt ccatgtgagg attgcttctc ctcctttgaa atatccatgt ttttacggga
     5341 ttgatattca aacgcgtaaa gaactcattg cggcaaatca cacgattgat gaaatcaaag
     5401 aatgtatcgg tgcagattct ttaggttttt taagtgaaga gggtctgatc gaatcgatcg
     5461 gtctggaaaa agatgctccg tattcaggat tgtgtatggc ttatttcaac ggagattatc
     5521 cgacgccgtt atatgactat gaagaaaaat atcaggaatc actaaaagaa aaagtatcgt
     5581 tcttttaaaa gcagaccaag aagggagtat atgaattcaa tggcaaatgc atacgcaaaa
     5641 gctggcgtag atgtcgaagc tggatatgaa gtagtagaac ggatcaagaa gcatgtcaaa
     5701 aaaacggaac gtttaggtat gatgggcgct ataggaggat tcggcggttg ttttgattta
     5761 tcggtctttc aattaaaaga gccagtcctt gtctcgggga ccgatggcgt ggggacgaaa
     5821 ttagcagtgg caatcgaagc agacaaacac gatacaatcg ggatcgactg cgtagcgatg
     5881 tgtgtcaatg atatcttagc acaaggcgca gagccgttat atttcttaga ctacttagca
     5941 acggggaaaa atgatccgtt acgtttggaa aatgtagtaa aaggtgtagc agatggatgt
     6001 gttcaaggaa atatggcgct gattggcgga gaaacagctg aaatgcctgg tatgtactca
     6061 ggtaatgatt atgatctagc cggatttgct gtagggatag ctgaaaaatc tcaattattg
     6121 acaggagaaa cgatcaaccc aggagatgtg ttgcttggaa tagcatctag cgggattcat
     6181 tcaaatgggt actctcttgt tcgcaaaatt ttctttgagc agcagcagtt ttctttatca
     6241 tcaaaactgc cagaattgaa aaaaacactt ggcgaagaat tgcttgagcc aacgaaaatc
     6301 tatgtttctt ccttgcttcc gctgatcaaa gaaaagaaaa taaaaggagc tgcacatatc
     6361 actggtggag ggtttgtaga aaatatcccg cgaatgttac cagaatctat ggctgcacaa
     6421 atcaaacttg gctcatggcc gattcttcca atattcggct gtttacaaaa atatggtgaa
     6481 attcctgaga tggagatgta tgaaatcttc aatatgggca tcggtatggt cctggcagtc
     6541 gaagaatcga aagtaaaaga aatagtacac gaaattgagc aagctggcga aaaagtttat
     6601 gaaatcggga aagtgatcgc caaagaaaaa catcgtgtgg tgtttatagg agaagaacga
     6661 tgagaatcgc tgtttttgct tctggaaacg gaagcaactt tcaagcgcta gccgattatc
     6721 tatcgaaaaa aggattggaa tcttcgattg actggttgtt ttgtgatcag cctgaagcat
     6781 atgttttgaa acgtgcaact gcgttgagtg taccggcaga ttgtttttca cctaaagaat
     6841 ttgattcaaa aaaagagtat gaagaagcaa ttttgcataa attgaaagaa aaaaagatcg
     6901 atttgatcgt attagctgga tatatgcgaa tcattggacc ggtattgctg aaaaattatg
     6961 ataaaagaat catcaatatc catccgtctc ttttacctgc cttccctggt ttgcatggca
     7021 tacgtgatgc ctttgaagct ggagtaaaag aaacgggtgt cacaattcat tatatcgacc
     7081 aaggtgtaga tactggacca atcatccggc aggaaaaagt gagaattgaa caggaggaca
     7141 cgttcgattc tttagaagaa aaaatccata gagtagagca tcgaatctat ccagaagtga
     7201 ttagtgaaat tattgaaaat ggaggctatc tgaaatgaca agagcattga tcagtgtatc
     7261 agataaaact gggattgtag cattcgccca aggtttgatt gaacaaggag tagaaatcat
     7321 ttcaacagga ggaacaaagc atgcgttgga taaagctggg atcaaaacgc tttcgatcga
     7381 agaagtgaca gggtttcctg aaatgatgga tggccgtgtc aaaacacttc atcctaaaat
     7441 ccatggtggt cttttaggaa gacgcgatct ccctagccat atggaggcaa tggaaacgga
     7501 aaatatccaa ccaatcgact ttgtttgtgt caatttatat cctttcaaag aaacgattat
     7561 gaaaccagat gtcgaaatag cagaagctat tgaaaacatt gatatcggtg gtcctagtat
     7621 gctccgtagt gcagcaaaaa accatgaatt tgtcacctct gttgttgatc catctgacta
     7681 tgaagaagta ctttcagaat tacgtcagta tgggcatact tcactttctt tcagacaaaa
     7741 actagcggca aaagtctttc gtcatactgc ggcatatgat gcactgatcg ctcaatactt
     7801 aacggattgg gtggaagaaa aagaaccgga aaaattgaca cttacgtatg aacgaaaaca
     7861 aacgcttcgt tacggtgaaa atagccatca agccgctgcc ttttatcagt ccgctcttct
     7921 ggaaaaatat tcgatcgcag cagcaaaaca actccatggg aaagaacttt cttataataa
     7981 tatccgagat gcagatgctg cgctacggat catgaaggaa tatgacgagc caacggtagt
     8041 cgccttgaaa cacatgaatc cttgtggtat tgggacagca tctacgattt tagaagcgtg
     8101 ggaagcagcg tatgaagcag atcctgtttc aatctttggt gggatcatcg ttttgaatcg
     8161 agaagtagat ctgccaacag cagaacagat gcataagctg tttctagaaa tcatcattgc
     8221 cccttcttat acacaagaag ctttggaatt actgcaaaaa aagaaaaaca tccgattact
     8281 gacagtagac tttgcgcagg aagaaaatca aaatcgagaa gaaacggtct cagtcttagg
     8341 tggacttctt gtacaagatc aagatctggc aatagaaaat gaagaagagt ggaaagtcgt
     8401 gacaaaacgt aagccgactc cagcagaagc aaaagcatta gtgtttgctt ggagagctgt
     8461 caaacatgtg aaatctaatg cgatcgtact ggcaaacgaa cgtcagacag taggaatcgg
     8521 agccgggcag atgaatcgag tcggttcagt aaaaattgcg attgaacaag cgcaagagaa
     8581 aatggaaggt gcagttttag ctagcgatgc ttattttcca atggatgatt cggttgaata
     8641 tgcagccaaa catggtataa aagcaatcat tcagccagga ggaagcatca aagaccaaga
     8701 ttctattgat atggcgaata aatacgatct agcaatggtc tttactgatg ttcggcattt
     8761 tagacattag agaggagtcg gataaatgaa cattttggtg atcggtgcag gtggtcgtga
     8821 acatgcaata agtaaaaaac ttttagccag tccgaaggtg gatacggtgt attgtgcgcc
     8881 aggaaatccg gggatgacaa aagatggtat caattgtgtc gctatcagtg agcgagatca
     8941 ttcagcgtta atccattttg ttaagaaaga ggctattgat tggagtatcg ttggtcctga
     9001 agttcctttg ttaaatggga tcgtcgatga ttttatggcg gaaggattga aaatcttcgg
     9061 tccgacaaaa gcagcagctc tgatcgaagg ttcgaaaagt tttgctaaac aattaatgga
     9121 tacttatcaa atcccgacag ctaaatccag aagttttgaa tgttacgaaa aagcagcagc
     9181 gtatatcaaa gaaaatggtg cgccaatcgt agtgaaagcg gacggcttag cagctgggaa
     9241 aggtgtagtc gtggctacca cagtgaagga agcgctggat gcggcagaac aaatgttgaa
     9301 ccagcatcga tttggggata gtagcaaaaa aatcgtcgta gaagagtttt tggcaggaga
     9361 agaattctcc cttttggcgt ttgtccgtaa taccaaggtg tatccgatgg tcatttcgca
     9421 agatcataag cgggcatttg ataacgataa agggccaaat acaggcggaa tgggtgctta
     9481 ttcaccagta ccgcaaattt caaaaaagat gattgaaaaa gctgtggaag aagttttaaa
     9541 accagcggct ttgggaatga aagaactagg acgctctttc acgggtgttt tatatgcggg
     9601 tttgattgct acgaaggaag gaccaaaagt gatcgaattc aatgcgcgtt tcggtgatcc
     9661 tgaaacgcaa gtcgttcttt ctcggttgaa gagtgacttt gcgcaagtaa tcgatgattt
     9721 attagagaat cgtacagtag agttaaagtg gcagcaagaa ggatacaatg taggcgttgt
     9781 ggttgcagct gccggttacc ccgaagaata tgagacagga atggttctac cagattttct
     9841 ggaagaagag ctttctgttt actatgctgg agtttcctca aataacggca aactagttgg
     9901 agcaggagga cgtctttatt tagtagaagc atacggtgag acgatcaaag aagcacagaa
     9961 aaaaatttac accgcgttaa tggataaaac gacagatgga acattttatc gtacagatat
    10021 tggagaaaaa gctgccaata gcgaactgat cgtgaagtaa atcagctgct tccatttata
    10081 aaaaatacaa caaacataat cggaagcgcc gattttagtg aagccttttc ataaagagtg
    10141 tggtacactt ttgttgtgta tataaataag gaggagttca gatgaaaaaa attcaattag
    10201 gtacttctga tcagaaagca gctgcagtga tcttaggctg tatgcgtatc aacaatgcgg
    10261 aagatccagt aaaagtgatt gagacagctt atgaaaacgg cattgatttt tttgaccatg
    10321 cagatattta tggaaacggt gaatgtgaag aaatctttgc agatgctcta gcaaaaacat
    10381 caatcaaacg agaagatctt tttattcaaa caaaatgtgg aatcgttcca ggtgtgatgt
    10441 ttgatttttc taaaaaacat atcattgaat ctgtagaaaa aagtttgacc cgtctgaaaa
    10501 tggattatgt ggatgcttta cttcttcatc gtccagatac attggtagaa ccagaggaag
    10561 tagctgaagc gtttgatgaa ttggaacggt caggaaaagt tcgttatttt ggcgtcagca
    10621 atcaaaaacc aatgcaaatc gaattgctga aaaaatatgt tcgtcaacca ttagtagcta
    10681 atcagctgca atttggcttg aaacatactg gaatgatcga tcaagggatt catgtcaata
    10741 tgacagatga agcaagtatc gatcatgacg gtagtgtact tgattattcc cgattgcatg
    10801 atatgacgat ccaagcatgg tcaccatacc agtatggatt ttttgaaggt gtatttatcg
    10861 ggaacgaaaa attccctgaa ttaaaccaaa cattagaaga actagcagct aaatatcaaa
    10921 cgacaccaac tggcttggct tctgcttgga ttttacgcca tccagccaat atgcaagtga
    10981 tcgccggtac gatgaacgta gggcgaatca aggaaatcgc tcaagcttca gaaattaata
    11041 tgtctcggga agattggtac gaattgtacc gtgctgctgg aaacatttta ccttaaagat
    11101 tgtcataagc aggtcaaaag ggctataaat taaaaagaca gaataacttg aaagctgttt
    11161 tcagcttcga gatattctgt ctttttttat tgcaaatgga aagagtatca ttgtacggat
    11221 ttcgtttatt agttattttt gtttttaagg aaaagctgtg tccgatattc ttttggagaa
    11281 atcccttgtt ttttcttaaa taaataactg aaatagttag ggtcattata accaatttca
    11341 aaagcaatat ctgaaatacg atagtctgat tgctgcaata atttttttgc taattcgatt
    11401 ctttgttctg tcagataatc gatgaatgtt tggttaaacg attgagaaaa aatcgtactg
    11461 aaatgagagg gacttaatgc aacttgttga gcgacccaag ttaaggacat atctggatcg
    11521 gtaaaatttt ctttgataaa atccaatgca tggttcaaaa ttggctgata tttgttcctt
    11581 gaaggctgtg agggcagagc agttaagaaa gaaatgaaca tataaactgt cgaacggtaa
    11641 agcgagtcgt cggcgctgat tgctaagcgt tgactggtgg atgtgcaatg aaccatttct
    11701 tgcgggattt tcttttcttt caggcagaca tgctggagta gcttagtcaa gacaaataaa
    11761 cgatataatc gtgttcgagt tggtgtgcct tgtggtttcg tcaagtgttc gatgtactta
    11821 gatacttcgt ttttcgataa tgaagacaaa tcaagtgaga ggtcaaagta aaagacagaa
    11881 tcaactggaa gtttcgcttc ttgaatcaaa tcctcatagc tgataatttg ttctttaagg
    11941 gaactggcgg tttcaagtat catctgcgtg atctcaaaag atgcaggtaa ttcgctgatt
    12001 ctttctgcga caggaccaat ggcgacagaa aaattatttt tttgtccctt ttctaattca
    12061 tggaccaacg tttgggctac ctgataactg gtctcgatca cattttcttc aagagaatca
    12121 actattaaaa atttgatgaa tcgagaggaa acagcagaga agatcacttg ttgattctcc
    12181 ctaaataaaa acttcaaata atcactgaga tgagcgtaat cttcaaagtc tgtactgact
    12241 tggtttgctg ctaataatac ggtcattttt ttccctgcta aagagcgatt gaaagattca
    12301 gcaagtttca atgtttcact gatcgtaagt gagcctttga acaaaccgtt caagaaatga
    12361 ttttttttca attcgaaaac aagatcagaa gaagtgtgcg tttctttttg ctgcaaatct
    12421 aaagcagtta ctgctttttc tattgcttgt ttcaattcgc tacttttaat cggtttaagc
    12481 agataatcat ctgcctgtac ctggattgct cctttcatat aatcgaaatc gtcgaatcca
    12541 ctaataaata tgagacgagt ccaaggaagt agtttccttg cttcttttga aaaagtcaac
    12601 ccgtccatga aaggcatttg gatatcagtc agaatgatgt cgggctgtaa gtcgataatt
    12661 gaagacagtg ccatctcacc atctccagct tcgccgacat aagtgattgg ataataagaa
    12721 ccaaggacag ttatttgttc ccgcaagtta ttgcgaatga ggtattcgtc ttcgatgata
    12781 aaaactttat acatgtttgt ttcctccaat tgttttggga gatagaggga tcatcagtgt
    12841 gacagttgtt ccttctcggt atttgctgtc aatcgtgata ggtttttgat catttccgta
    12901 ataaagaagg agacgcttgt ttacgttgta aagtccgtag ccgatactga aatctgaatc
    12961 gatccctttg gctaactctt tacgtatttc tgccagtttt tctgcaggga tgccaatccc
    13021 gttgtctata actttgaatt ggatcatctc atcaattttg catgtggtaa tacgaataaa
    13081 tcctttgtga cggacgtact tcgttccatg gtatacagca ttttcaatca atggctgcaa
    13141 gatcattttt aaaatagaaa caggaaaaag tgtttctggt atatcgatct tatagtcaag
    13201 catatggccg tatcttattt ttaagatgac tagataatca ttgatatggc taatttcttt
    13261 ttcaagagga atccagtctt gtcctttgct taaagacgta cgaaaaaagc cggaaagtgc
    13321 atgggtcata gtcagagctt cttcaaagca gtcttgttcg atcaaagaaa taatggcatc
    13381 caaactgtta taaataaaat ggggagtaat ctgagcttgc aatacacgaa cttcactttg
    13441 tgctaaatgg tattgtttca aagcgttctc ccgtaaagac tggtttagat cttccgccat
    13501 tttattcatg ctagcggtaa gtgtttgtaa ttccagtgtt tccggcaggg ttaaacgaaa
    13561 gccaaggttc cctgaagaaa gttgttctga catctcgatc agctgattga tcggttcttg
    13621 gacctttctc tctaaaaagc gacgattctt tttcgcatag aaaaaaatgg tactaagaac
    13681 tccaagttcc actattgtta atagaatgag cgagcggaca agtgtcgtat tttttttgct
    13741 tgctaagtcg atctccacac gaacaaaatc ttgtaagata tctgacagca gttgagtgac
    13801 agaatctacc tgattgatga tctcttcatt ggtatccata aaatcttttg ccaagatatt
    13861 attgatcagc tcgttttgat aatcttccag tgtatcgatc gtacgtagcg atacatctaa
    13921 cgtactaatt tctttaagtg tatttgtatt atttttgctc tctcgaatat cattcctaag
    13981 ttcttctagg atattttcct cattgaactc atctacggtt acttggccag aaatcaagtc
    14041 ccacatactt tccaaaactt tttgatcaac cttcaaagca attgcatctg ctttttggat
    14101 atttgcgatc gtctgttgat aaatcaaaag ctgttgcgaa tataacagta tgctcagtag
    14161 caaagggata acagcaaata gaagcatggc tttgtttgct tgggcaaaaa tcttttttat
    14221 gcttaaattt tttgtcatga tacttctcca gtcaataatt tctggttggg atgttagcta
    14281 tattctgctg ggaaaagaca gtctcggaaa taaaaatatc tttaggtaat gtttttcctg
    14341 tttgaatatt tccaaaataa cgagtaacgg tattggctac ataccagccg acatcaggtt
    14401 tacactcgac tacacaattg acgattcctg agcgtagctt ctcgatcata tcttcttgtc
    14461 catcaatagt tacaatgatc aaatcttctc caggtttgat tgatgtttct tttatcgcag
    14521 ctaaagcacc gtgagtcatt tcatcactat ggctgtataa tacatcgatt tcggataaat
    14581 catttgtttc taaatatcgt ttcatctggt cttttccttt catccgaata aaatcaccga
    14641 caattcggtg aacaatagtc atcggaggtt tatggtcgat ggttccatct ttttttaggg
    14701 ttggtttacg attgattgat tccaaaaatc cttcggagcg tagcttggtc ggtgtggagt
    14761 tctctgaacc agcaagttct aggattctga tttcctgttt ctttgaattt gtgaagtgat
    14821 tgctgacaaa taaacccgcc cgatttcctt ctgctttgaa actggggcca atgtgggtga
    14881 cgaaaagctc gggatcattc gtttgaatat gtctgtcgac aagtatgaca gggatccctg
    14941 ctcgctttgc ttcttttaat acagagtccc acccggattc ttgcaaagga gtaaaaatga
    15001 tcatatccac tttataggca atgaacgtcc gtatatcttg gatttgccgt tcctgattca
    15061 tgtagccatt tcggtagaga acttggtaat cttctttttc aagcgcagtg ataattgatt
    15121 cagtatgctt tttccgccag ctactctccg ttcctgattg cgaaaaacca acgactagtg
    15181 ggtcaggtgc ttcctttgta ttaggtgtac accccatcat ttggtaagcg attacaacaa
    15241 gggctaaaaa aaataaaaca cgtttcactt taaatcactc catctggatt tgttagtact
    15301 atcttatcag ataaaaggga aaatcaaagt caaaaggaat caatcgaaaa aaatccaaga
    15361 aacttaaaaa aatccaagac aaaaaaagta cacctaaaaa atttaaagta aatcccataa
    15421 aaaaatcaat gttaacgctt tctttattag ctatactcgg tgtatcaatt gaaatggggg
    15481 ataaaaaatg aaaaacaaat ttagaaagat tgttgggact atcgctattt tgaccgcaag
    15541 cattctatta agtgcgtgtg gcaattcagg atctgcagat gatagtacgg ggtatgtagg
    15601 aatcgctatg ccgaccaaat cagcagaaag atggatcgct gatgggaaca acatggtctc
    15661 ggaattagaa aaactgggtt ataaaacaga tcttcaatat ggagaagata aagtagaaaa
    15721 tcaagtagca caaatagaaa atatgattac aaaaggggta gatacacttg tgattgcttc
    15781 tatcgatggt tcggctttaa ctgatgtgtt agctaaagca aaagaagcag atatcaaggt
    15841 gatcgcctat gatcgtttac tgatgaattc agaaaatgtc gattattacg cgacttttga
    15901 taattttgga gtaggtgtat cgcaagcagc ttatattgaa gagcacttag gattgaaaga
    15961 aggaaaagga ccattcacga ttgaattatt tggtggctcg cctgacgaca acaacgcact
    16021 aatcaattac aacggagtga tgtctgtttt acaaccatat atggacaacg gtcagttggt
    16081 cgtgccatct ggacagacga gtttcagtca aatcgctacg cttcgctggg atggttctac
    16141 ggcgcaagca aggatggata atctgttaag cgcaaattat actgaccaaa cattggatgc
    16201 tgttttgtcg ccatatgatc caataagttt aggaatcatt tcgtcattaa aaggcgtggg
    16261 gtatgggagt gaaagtaaac ctttacctgt gattactgga caagatgcga cagttgctgg
    16321 cgttaaatcg atcattgccg gggaacaaac acaaacgatc tttaaagaca cacgtatctt
    16381 agcaaaaaat acgattgaaa tgatcaaagc aatcagtgat ggagaagagg ttccggtcaa
    16441 tgatactgaa acttatgata atggggtgaa aactgtccct acgtatttag caaatacagt
    16501 atctgtcgat aaagataatt atcaagcaga gctgatcgac accgattatt ataaagaatc
    16561 cgatctgaaa aattaagagg aaccaaacaa ggaactaaag agatctaagt cagagagagg
    16621 ataagaatta tggcggattg tattttaaaa atggcagata tcaccaaaaa attttctgga
    16681 atccgagcgt tagattgcgt gaatctgatg attgaacgtg gggaaatcca tgcattgtgc
    16741 ggagaaaacg gagcgggaaa atcaacattg atgaatgtct tgagcggctt gtatccatat
    16801 ggctcctatg acggggaaat cttttatgat ggaaagctgt gtcaatttaa agatttgaaa
    16861 gatagtgaag aaaaaggcat cgtcatcatt catcaagaat tagcactaag tccttatctt
    16921 tcaataaaag agaatatttt tctcggaaat gaacaagcag aaagaggcgt gattgattgg
    16981 gaactgacag aaaagaaaac atatgaactg ctgaataaag tcggtttgtc tatcgacccg
    17041 aatcttctag tatcccagat tggcgtaggg caacagcaac tagttgaaat agctaaagca
    17101 ttatcaaaat ccgtccgtct gttgattttg gatgagccta ccgctgcatt gaatgaagaa
    17161 gaaagcgcga atttgctgag attgatccga gagttgaaag agcaaggtgt aacctccatc
    17221 attatttcac ataagctgaa tgaaatcgtg gatatcgcag atcgaatcac cattttacgg
    17281 gatggacaga cgattgagac actagaaaat gaaatgatca gcgaagagca tattattcgc
    17341 ggaatggtgg gcagagattt agttgatcgt tatcctgaaa gaaaaccgga tattggagaa
    17401 gtttatttta aagtagaaga ctggacggtc tatcaccctc ttgatcacga gcgaatcata
    17461 aacgaccaca tcaacttgca tttgaaaaaa ggagagattg taggaatagc tggactgatg
    17521 ggagcgggca gaactgaatt cgctatgagt ctatttggac attcttatgg aagcaagata
    17581 tccggacgta tattaaagga aggggcagag atatcagtta agaccgttcc ttcagccatt
    17641 gcaaacggac tcgcttatgt ctcggaggac cgaaaatctt taggactgaa tctgctgatg
    17701 gatattcggg aaaacgtgac gctgagcagt ttggataaaa taagtcaaaa aggagtatta
    17761 gataaagaaa aagaagtaac tgaggcagaa aatttccgga aaaagatgcg tataaaaaca
    17821 aactccatcc atcaaattgt cagtagtctt agtggtggga atcaacaaaa agtcgtttta
    17881 tccaagtggc tgatgacaca acccgatatt ctttttttgg atgagccaac ccgtgggata
    17941 gatgttgggg caaaatatga gatctacacg attatcgaag aaatggcagc aaatggcaaa
    18001 gctgtctgtc ttatctcttc tgaacttcct gaaatattag ggatgtgtga tcgaatctat
    18061 acgatgaacg aaggacgtat tacaggagaa gtctcgcgag aagatgcaga tcaggaagtc
    18121 ttgatgaaat taatgacgaa agaagaggtg ggttaagttg gaacaggcaa aaaatcttac
    18181 ggataaaaaa gagaaaaaac taaatttgaa aagtagagca gctgatcttt ttggtcgcta
    18241 cagcatggtg atcattttag cgggactact tattgcgttt caattgatga cggatggtat
    18301 tttctggcgt ccattgaata taactaacct tgttctgcaa aatagccata tattagtact
    18361 agctgcaggg atgttattag ttgtgctctt aggtcatgtg gatttgtcgg tagggtcagt
    18421 gatggcgttt gttggtgcaa ttgcgggcat gatgatggtc aatcgccaaa tgaacccgtg
    18481 gatgacagtt atcgcatgtc ttgcagtagg cgggctgatc ggcgcatggc aaggtttttg
    18541 ggtagcttat gcagggatac cggcgtttat cgtgacttta gcaggattgc tgatgtttcg
    18601 tggactgaca caagttgttc taggaggcca atctctagca ccttttccag ctgttttcca
    18661 aaaaatgtct acgggttatt taccagactt aagcgaagga agcagccttc acttcttgac
    18721 attgatttta ggaatgatca tcacgatcgt gttagttgtt ggtcaatggc gaacaagaaa
    18781 tcgtcggaaa aatcatttat ttgaagtgga atctccaaca gctttcttgg taaaggcggt
    18841 atttacagca gtcgttgtac taggaataac ttatatcttt gcctcttatc aagggtatcc
    18901 gattattttg atcattcttg gtttgatcgt tgcagcatat gggtttttga caaataaaac
    18961 agtagcaggt cgacagattt atgcgacggg cggaaatcga aaagcggctg aactatcagg
    19021 gatcaaaaca aagcgaatca cgttctgggt attcgtcaac atgggagtca tggcagcttt
    19081 agctggatta gttttagctg ctcgattgaa tgcggcaaca cctcaagcag gtacatcgat
    19141 ggaactggat gcaatggcgg cagtatattt tggtggtgct tcgacttccg gcggcattgg
    19201 aacaatcatg ggtgcgattg tcggtggctt ggtcatgggt gtattgaaca atggcatgtc
    19261 gattttaggc gtcggggtag actggcagca ggcaatcaaa ggattgatct tgcttttagc
    19321 cgttgtgcta gacttgtata ataagaaaaa acgaacaacg taggagaacg ccggttattc
    19381 agaaaaaaga ctgtgacaag acatttgtca cagtcttttt ttcgctaaaa gcgaacattc
    19441 attgtttcgt ttgtatagac taaaaataat atatgttttt ttaccattcg tataaattct
    19501 ctttttgctc ttgatttttt tgcttaattg ag
//
LOCUS       DAZKLD010000045        19451 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.045, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000045 DAZKLD010000000
VERSION     DAZKLD010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19451
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.045"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            127..3654
                     /locus_tag="ACG8KR_001287"
     CDS             127..3654
                     /locus_tag="ACG8KR_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HFQ0200666.1"
                     /translation="MSLQFILGNGKKDHRQVLIDEASAWLDQDASNQVFFLVPNYSKF
                     EQEQEILAEMRRRSGKKTFSTTNVQVFSFYRLAWYLLQQTTLLTGNELSESGSAMILR
                     QILEKNEEELTIFRGEINKRGFIRQLQELYSELRTGNIQPEDLMLLFGDSPNAEDQQL
                     KMKDLKLIFSAYDSELTQRALTNEDALSILANYMQTQDFNNVKFIVSGFSRINGQEYQ
                     LLQAMMEKGQLVVDLLLDRPYTADLPEVLTLFHETGKLYFRLFQSARERKVPILVDRF
                     APEQTRPTELQLLQTMWEETSSQKKAAVHKIGGQEHLHLWEAENPTEEVRQLAVEIRR
                     LVSEENYRYRDIQVLTKNMSLYGNILRPIFQEMAIPFYIDEEQMMENHPLIEWINSLF
                     ALDRYSYRLSDIMRFFKTELFLSTEAETELDEWKQERDEFRKKLDITENVALAYNYQG
                     TYWTREKDWQFVAYDFEAEQIEDVQYLEEQSNTIRRLFQKAVPVFLKKIKKSKTGQEA
                     ANVFYQFLIQSGVQQQLLFWRNQEVDRGNLEAARNHEQTWGALIDLLDEYVVIYGESA
                     FDWSLFQEIITSGLENLSYGKIPTAIDQVRINRLELVRPNQAAVTFALGLNDQVFPDR
                     KETKGLLSSEEREVLNQALPDGQFLFDPSKESISFEPFQAYLVFLSGTDRLYLSYAQS
                     YDTDGSLKMSPYLRRITEYLSVPVERKEHLTIESDPNCYVGTYRSGINTVNRIYRLAL
                     DEKKPVPKHWQKLKELVLNSSWRNFALQIFESQEHLNVPVSMPEKMAEELYGKEIYTS
                     ISRMENFYNCEYKYFMQFGLKLKERTVYGLTPAATGDFYHESLDRFFKLLFSNQLSLV
                     NMSDAQRRDFTEKVLQEVFGELRFEILDSSARMNYIRYQLGQTIQKVTWALQKQSKNT
                     GMHPLQTEILFGQIAGAKGITGLELPLSNGGKLHVRGKIDRIDVASEQEDTWLSVVDY
                     KSSGRSFDVTEAYYGMAMQLLTYLDVALMDAVRLTGKAAVKPAGSYYLHVHNPVLTET
                     DDVEKNTLKKFSYDGVFVDDPALLEVLDHSLEAKEQSLIYPVKKNAKDEYQKGSFSKD
                     KFFTEEELQLFMAHNRENMRLAGDAITSGEIKLNPAYKDKQRIACEFCPFRSICTFDV
                     MLKENNYHRLEKIDKEEALRRILKRSEEE"
     gene            3651..7373
                     /gene="addA"
                     /locus_tag="ACG8KR_001288"
     CDS             3651..7373
                     /gene="addA"
                     /locus_tag="ACG8KR_001288"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0000724 - double-strand break repair via
                     homologous recombination [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HFQ0200667.1"
                     /translation="MNQIPLKPQNERFTDDQWQAIFDQGDNLLVSASAGSGKTTVLVR
                     RVIEKLKMGFDIDELLIVTFTEAAAREMKERIQEALQESVNSESDPVRRQHFTKQLVL
                     LPTANISTLHAFCLTVIRRYYYLIDIDPVFRMLTDETETILMKEDVWDELREALYAEN
                     DERFFQLTMNFSNDRSDDGLTNLVFSLYEFARANPDPQKWLEQLSDNYRLPEGLAKSR
                     LYQKQIRPLVLADIYQCVQLYEQMTQLAQGEGLEKMHEQVAGEQQQIKNIYEAFSQDR
                     LEEAYAGLEQLTFSTFKSSRKAELKEISNEVKGMRDKAKKLIQQISKSYFPVSPSQME
                     ELTDKALPLVEEMTKVTQSFMDGFSMRKREKGVLDFNDLEHLALQILTEKTKDVWLPS
                     EASKHYRKKFKEVMVDEYQDVNQLQEAILYWLREPDDTKGNMFMVGDVKQSIYSFRLA
                     DPSLFIGKYENFSKKEGGRRIVLAENFRSRKEVLSFTNLIFEQLMDPAVGQINYDEAA
                     KLIQGFSDFPENEQFEPEIMIYEKEQEESEIEIPTDDILEDKTEGELFMTGLKIRQLI
                     DSSFMIYDKKSKKSRPIEYKDIVLLTPTKKNNLTILEIFKTLDIPLEMNDAQNYFQAT
                     EIRTMISLLQLIDNPYQDIPLAAVLRSPIVGLIEPELASIRLANRAHTYYDAVLAYQA
                     SNEDELAAKLEHFGKQLEHWRELARRSSITDLLWDIYYETGYLEYVVGLPAGAQRQAN
                     LYALVDRAKAYEQSSFRGLYQFVRFIEKMQEKDKDLAEPVISIEDNAVRVMTIHASKG
                     LEFPVVFLLDMTKEFNLQDLRNRYAFEEKLGAGIRYMDPETRVLYDTLPFQAIKLAKQ
                     NKLLSEEMRKLYVGLTRAEQKLFIVGSYKNKEQMIQTWSEAADHEELVFDPALRLKGR
                     SSLMNWIGYGLIRHPEMQKYLEEEISTSLLQHSNAQFSISWMNQQSIIEQRQLLAEKE
                     LVNLDQQMKEDETLLADSLQKRLAYEYPYQASSQTTSYQSVSEIKRLFEDPDDTQESR
                     LTLESSQNKAASRQFRYTQEQLAEPKFLQKDRQVSAAAVGTATHALLQLLPLEMPTTE
                     SIHQKLQELVRKRLVDEKVAKKVDVSSIIWFFQTELGQQLIANKENVKREQPFSMLLP
                     ADEVFQDYPNQEDELLIHGIVDGYLEEKDHLNIYDFKTDFILHPDDPAEIDAIVQKYQ
                     GQLRLYQQAMSEALNKPVENVFLILLRVKQIININK"
     gene            7442..7783
                     /locus_tag="ACG8KR_001289"
     CDS             7442..7783
                     /locus_tag="ACG8KR_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HFQ0200668.1"
                     /translation="MELTQTEFVLCPKFEKTFSILGKKWNGLIIDVLLENGPQRFVEL
                     AAKIPMVSDRVLVERLKELEKEGIVTRNFACSEKSRSEYLLTEKGADLQKAMRQIQVW
                     SEKWITEEECG"
     gene            8092..9135
                     /gene="pheS"
                     /locus_tag="ACG8KR_001290"
     CDS             8092..9135
                     /gene="pheS"
                     /locus_tag="ACG8KR_001290"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HFQ0200669.1"
                     /translation="MSLQAQLESLKEATLVKITAAQDLNELNQIRVETLGKKGPITEV
                     LRGMKDLSPEERPKVGSFANEIRDLLTEEIVRKKQVLEEEALNAALAKETIDVTLPGR
                     KVKHGTRHIVTQIMEEIEDIFVGMGYQIVEGYEVESDHYNFERMNLPKDHPARDMQDT
                     FYISDEILIRTHTSPVQARTMEKHDFSKGALRMISPGKVFRRDTDDATHSHQFHQIEG
                     LVVDKNITMGDLKGTLEVVMKKMFGEDREIRLRPSYFPFTEPSVEVDVSCFKCGGAGC
                     NVCKYTGWIEILGAGMVHPNVLKMSGIDPEEYSGFAFGLGPDRVAMLRYGVNDIRNFY
                     QNDLRFLSQFKGE"
     gene            9140..11560
                     /gene="pheT"
                     /locus_tag="ACG8KR_001291"
     CDS             9140..11560
                     /gene="pheT"
                     /locus_tag="ACG8KR_001291"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HFQ0200670.1"
                     /translation="MLVSYKWLNRYVDLSNVTPKELADKMSVTGIEVEGVTVPEEGLK
                     KIVVGEVKECVPHPDSDHLSICQVDIGEDELSQIVCGAPNVKVGIKVIVALPNSRIAG
                     NVKIKKGKMRGQVSNGMICSLQEIGYSDSVIPKEYAEGIYYMPQEAVNGDPVFPYLDM
                     DDSIIELSITPNRADALSMRGVAYEVGAIYRQTPTFDDEKLVESSRPASDKIQVEVED
                     KEAVPAYQIRIIEGVRIQPSPQWLQNLLMNEGIRPINNVVDVTNYILLLFGQPLHAFD
                     YDKLKSKEIFVRRAKENETLVTLDGESRELTTDNLVITNGKEPVALAGVMGGLDSEIT
                     NETTTVALESALFDPISIRRTAKQFNLRSESSARFEKGINHATVGEASEVAAAMIVSL
                     AGGEVLQGAVKGSEIKAEDVSVKISLERINQYLGTSLVVKEVNEIFEALGFSYEEKEY
                     VYSVVVPPRRWDIQIEADIIEEVARIYGYDQLPSTLPSGETVAGSLSKEQETTRKIRT
                     IFEGSGLSEAISYALTTEEKSRQFVINESNITRLDWPMSEERSVLRMNLISGLLDDIS
                     YNTARKNTEIALYEIGRVFFQENDPRTHLPQEVKHAAIAISGIWEEKDWQTKAQNVDF
                     FTIKGLLENLFEQLGITEDVHYQTVTEMKEMHPGRTAAIYLGEKFIGFVGQVHPTITK
                     SYDIPETYVAEFDLSAIVEAAQKGIVFEAVTKFPAVSRDIALLVKETVTNQELTEVIR
                     SAAGRFLTDIQLFDVYQGENIEKGHKSMAYSLTFVNPEATLTDEEINKAMEKVEKALV
                     ENLEASIR"
     gene            complement(11730..12740)
                     /gene="trpS"
                     /locus_tag="ACG8KR_001292"
     CDS             complement(11730..12740)
                     /gene="trpS"
                     /locus_tag="ACG8KR_001292"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HFQ0200671.1"
                     /translation="MKNIILTGDRPTGKLHLGHYVGSLKTRVAMQADEDNQLFVMIAD
                     MQALTDNAKNPEKVSSNVLQVALDYLAVGLDPAKSTLFIQSQIPELAELTMYYLNLVS
                     VGRVRRNPTVKTEIEQKKFGESVPTGFFIYPVSQAADITAFKANLVPVGEDQKPMLEQ
                     TQEIVQSFNHTYGEVLVEPKGVFPPKGMGRLPGIDGNGKMSKSLGNGIYISDPADVLQ
                     KKVMSMYTDPNHIHVQDPGQVEGNMVFTYLDVFGTDKEAIEEMKAHYRRGGLGDVKIK
                     RYLIDVLEAEFAPIRARREELEKDPSAVMEILRKGSEEAAKVAAQTLSEVKSAMGINY
                     FH"
     gene            12994..13731
                     /locus_tag="ACG8KR_001293"
     CDS             12994..13731
                     /locus_tag="ACG8KR_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFQ0200672.1"
                     /translation="MAMIKFDHVDKYYGKFHALKNINLEFEKGEVVVVIGPSGSGKST
                     MLRCINGLETISSGKLLINDTDLHDKKTKLTEVRKNIGMVFQHFNLYPNKTVLENIKL
                     APIKVLKQDQATAVKNAEKFLKTVNMLDKKDSYPSMLSGGQQQRVAIARGLAMNPEML
                     LFDEPTSALDPEMIGDVLDVMKKLAHDGMSMIVVTHEMGFAKEVADRVIFMADGEVLE
                     DSRNVRDFFENPNEVRAQQFISKVINH"
     gene            13745..14560
                     /locus_tag="ACG8KR_001294"
     CDS             13745..14560
                     /locus_tag="ACG8KR_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HFQ0200673.1"
                     /translation="MRRLKKIRLIFLLGIAFLALAGCKGNGLANEDILERSKSTNEII
                     WGVKYDTRLFGMMDIESRTVQGFDVDIAKAITKKILGDNGKTEFVEVTSKTRIPLLKN
                     GNIDAIIATMTITDERKKQVDFSDVYFDAGQALLVKKGSQIKSVDDLNASTTVLAVKG
                     STSAANIRQHAPDAKILELENYAEAFTALQSGQGDAMTTDNAILLGIADENPEYELVG
                     GTFTNEPYGIAINKGQENFLKAVNQALEEMHADGTYDKIYQKWFPNETEGKVE"
     gene            14573..15220
                     /locus_tag="ACG8KR_001295"
     CDS             14573..15220
                     /locus_tag="ACG8KR_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFQ0200674.1"
                     /translation="MSELIQTYGPIFVDGFKITLFSSVLALVFSLIIGTLMAIFQLSK
                     NKVVSALAKAYVEFFRNIPLLIIVMFFYVVVPIYWFNLDGFQAGTIGLTIYTSAFIAE
                     TVRSGIQTVPKGQTEAGLSSGFTYSEIMRYIVLPQAFKIVIPPLGNQFINLVKNSSIL
                     AIVAGLDLMYQGDLIASETFNTFDTYIIVGLFYLVITLPLSYLMVYLEKKWAVRS"
     gene            15236..15892
                     /locus_tag="ACG8KR_001296"
     CDS             15236..15892
                     /locus_tag="ACG8KR_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFQ0200675.1"
                     /translation="MDFSGAFSWMNIRFLLEGLKVTVEVSLFSIIFSFLIGGLTGVIR
                     FANIPFFSKILGVVIDIIRNLPLLLIIFFTYFALPQIGIQMNIFWSAVAALTIFESAM
                     LSEIFRAGLNAVPKGQMEAGLSTGLTYVESMRTIVLPQAFKSMIPAIVSQLISLIKDT
                     SLAVIISLPELSHQARIIYGQNTNYVLPMFIIMTLMYFVVCYALSLLSGYLEKRRYSY
                     "
     gene            16054..16875
                     /gene="racE"
                     /locus_tag="ACG8KR_001297"
     CDS             16054..16875
                     /gene="racE"
                     /locus_tag="ACG8KR_001297"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HFQ0200676.1"
                     /translation="MIRLTDNRPIGFIDSGVGGLTVVKEALKQLPNENILFVGDTARC
                     PYGPRPAEQVIQYTWEMTDYLVEQGIKMLVIACNTATAVALEEIKAALSIPVIGVILP
                     GTRAAVKKTQNKQVGIIGTIGTVKSQAYEKALKEKVPELTVTSLACPKFVSVVESNEY
                     HSSVAKKIVAETLAPLTTKKIDTLILGCTHYPLLRPIIQNVMGENVQLIDSGAETVGE
                     VSMLLDYFNLSNSPQNGRTLCQFYTTGSAKLFEEIAEDWLGIGHLNVEHIELGGK"
     gene            16878..18233
                     /gene="rph"
                     /locus_tag="ACG8KR_001298"
     CDS             16878..18233
                     /gene="rph"
                     /locus_tag="ACG8KR_001298"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0009022 - tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HFQ0200677.1"
                     /translation="MRHDGRKAQELRKLELQTNVFKYPEGSVVIRFGDTTVICSATIE
                     NSVPPFLRETGTGWVTAEYSMLPRATSTRNRRESSKGKLSGRTMEIQRLIGRSLRAVV
                     DLEKLGERSIIVDCDVIQADGGTRTASITGAFVALKLAIEKLLREKELSEDPIKEHLA
                     AVSVGILPDGTCVTDLDYQEDSAALVDMNLVMTESGKFVEIQGTGEEATFDGEQLNEM
                     LFFGKNAIEDLIKEQKHALLTEFAQNDERIEETKTIIIATRNPGKAEEFRNMFKEAGY
                     HVKTLLDYPELPDVEETGSTFEENARLKAETIAQLLDQPVLADDSGLKVDALGGMPGI
                     YSARFAGEQKSDAGNNAKLLYELTDVPDEKRTAQFHCTLVFAAPKKDSLVVEAEWPGR
                     VARIPSGENGFGYDPLFIPEGKKQTAAELSSEEKNKISHRAQAMKKLSAEWKQWLEGE
                     R"
     gene            18234..18752
                     /locus_tag="ACG8KR_001299"
     CDS             18234..18752
                     /locus_tag="ACG8KR_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFQ0200678.1"
                     /translation="MRYLVVSDNHGDRDILVDLVDRYRSEVDIFFHCGDSELEASDPL
                     WDTFQVVQGNCDYGPGFEQKKVIQTGQDTVFMTHGHLSNVRFGLTQLAIEARAATADM
                     VFFGHTHQIGCEMDQKILFLNPGSISQPRGPIQIPSYALIESLDDQISVQYYNRAHQA
                     VEELAFSFSRER"
     gene            18859..19359
                     /gene="cbpB"
                     /locus_tag="ACG8KR_001300"
     CDS             18859..19359
                     /gene="cbpB"
                     /locus_tag="ACG8KR_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0180001 - cyclic-di-AMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP-binding protein CbpB"
                     /protein_id="HFQ0200679.1"
                     /translation="MIGPIVREMLLENQETFLIPAENVANVMYHHPLSHGLLVLSKVG
                     YSKIPVLGKDDHFVGLVSLSDVVNKMIDLQTISMDPLEGLTVADVTETDVPTIDENWE
                     LEEVLHLLVDSPFLPVVTEDRIFKGIITRKELLKAVNFMVHELERRNILSPKIEELKE
                     QIDIVS"
BASE COUNT         6540 a         3341 c         4177 g         5393 t
ORIGIN      
        1 cgtatactta aaaaagtaga aatatcagaa ccttaaaagg atataatttg aagaatatag
       61 aacaaatgtt cagtattttt tgatataata aaggagcacc gtatcatgtg ttgaaggagg
      121 aaaagcatgt ctttacaatt tattcttgga aacgggaaaa aagatcaccg tcaagtcttg
      181 atcgacgaag catccgcttg gctcgaccag gatgcaagta accaagtatt tttccttgtt
      241 cccaactaca gcaaattcga acaggaacaa gaaattttag cggagatgcg aagaagaagc
      301 ggaaaaaaaa ctttcagcac aacaaacgtt caagtattta gtttttatcg tctggcttgg
      361 tatttgctgc aacagacaac tcttcttaca ggaaatgaat tatcagaatc aggatctgca
      421 atgatcttgc gacagatttt agaaaaaaat gaggaagaat taacaatctt tcgcggagaa
      481 atcaataaaa gagggtttat tcgtcaatta caagaactct acagtgaatt gcgtacagga
      541 aatattcaac cagaagacct gatgctttta tttggtgatt cacctaatgc agaagaccag
      601 caacttaaaa tgaaagactt gaagctgatt ttttcagctt atgacagcga attgactcaa
      661 cgtgcactaa caaatgaaga tgcactatct atactagcaa attatatgca gacacaagat
      721 ttcaacaacg tcaaatttat tgtcagtggt ttttctcgaa tcaatggtca agagtatcaa
      781 ttactgcagg caatgatgga aaaggggcaa ttggttgtcg atttattgct ggatcgtcct
      841 tatacagcgg atcttccaga agtacttacg ctctttcatg agactggaaa gctttatttc
      901 cgtctttttc aaagtgcaag agaacggaaa gtccctatat tagtggaccg atttgcacca
      961 gagcagactc gtccgacaga gcttcaactg ctgcaaacaa tgtgggaaga gacatctagc
     1021 caaaagaaag cggcagtaca caaaataggg ggacaagagc atctccattt gtgggaagct
     1081 gaaaatccaa ctgaagaagt ccgacaatta gctgttgaaa tcagacgact agtcagtgaa
     1141 gaaaactacc gttatcgtga tatccaagtg ctgacaaaaa atatgtcttt atatggaaat
     1201 atccttcggc cgatctttca agaaatggca attccttttt atatcgatga agaacaaatg
     1261 atggaaaatc atccgctgat cgaatggatc aacagtctgt tcgctcttga ccgctattct
     1321 tatcggctaa gtgatattat gcgttttttc aaaactgaat tatttctgtc gacggaagca
     1381 gaaaccgaac ttgatgaatg gaaacaagag cgagatgaat tcaggaaaaa actggacatc
     1441 actgaaaatg tagcacttgc ttacaattat caaggaactt attggactcg tgaaaaagac
     1501 tggcagtttg tggcgtatga ttttgaagca gaacagattg aagacgtaca gtatttggaa
     1561 gaacagtcaa atacgattag acgattattt cagaaagctg taccagtatt tttgaaaaaa
     1621 ataaagaaaa gcaaaacagg acaagaggcc gcaaatgttt tttatcaatt tttgatccaa
     1681 agtggcgtac agcaacaatt attgttttgg cgtaatcagg aagtagacag aggaaactta
     1741 gaagcggcaa gaaaccatga gcagacatgg ggagcgctga ttgatctgtt ggatgaatat
     1801 gtagtaattt atggtgaatc ggcttttgac tggtcactgt tccaagaaat cattacaagt
     1861 ggtctagaaa atctaagtta tggtaaaatc cctaccgcaa tcgaccaagt gcgaatcaat
     1921 cgtttggaat tagttcgacc aaatcaagct gccgtcactt ttgcattagg gttgaatgat
     1981 caagtgtttc cagaccgaaa agagacaaaa ggattgttgt ccagtgaaga acgagaagta
     2041 ttaaatcagg ccttgccaga tggacagttt ttattcgacc catcaaagga aagtatctct
     2101 tttgagcctt tccaagccta tcttgttttt ctttccggaa cagatcgatt atatcttagt
     2161 tatgcacaaa gctatgacac agatggttcg ctgaagatgt ctccttattt gcgtagaatc
     2221 acagaatatt tatctgttcc cgtcgagaga aaagagcatt tgacgatcga aagcgaccct
     2281 aattgttatg tagggactta cagaagcgga atcaatacag tcaataggat ttatcgatta
     2341 gctttagatg aaaaaaaacc tgtaccaaag cactggcaaa agctcaaaga attagtactg
     2401 aattcgtcat ggcgaaattt tgctttacag atttttgaaa gtcaggaaca tttaaatgtt
     2461 cctgtttcga tgccagaaaa aatggcggaa gagctttatg gaaaagagat ttatacttca
     2521 atttcacgta tggagaattt ttataattgt gaatacaaat acttcatgca atttggtttg
     2581 aaattgaaag aacgaactgt ttatggtcta actccagcag caactggtga tttttatcat
     2641 gaatcattgg atcgtttctt caagctactg ttttcaaacc aattatcctt agtgaatatg
     2701 tccgatgcgc agcgtagaga ctttactgaa aaagtgctgc aggaagtatt cggagaactt
     2761 cggtttgaga tattggatag ttctgcaaga atgaactata tccgctacca gttaggacag
     2821 acgattcaaa aagtgacatg ggctttgcaa aaacagagca aaaatacagg catgcatcct
     2881 cttcagacag agattctatt tggtcagatt gctggagcaa aaggtatcac aggtctggaa
     2941 ttaccgttga gtaatggtgg aaaactccat gttcgaggga aaatcgatcg aatcgatgta
     3001 gcttctgaac aagaagatac ttggctaagc gtcgttgatt acaaatctag cggacgttca
     3061 ttcgatgtca cagaagctta ttatggcatg gctatgcaat tattgacgta tctggacgta
     3121 gcattgatgg atgcggtacg tttgactgga aaggcggctg ttaaaccagc aggatcatat
     3181 tatttacatg ttcataatcc tgtattgacg gagacagatg atgtagagaa gaatacattg
     3241 aaaaaattta gttatgatgg cgtttttgtc gatgatcccg ctcttttaga agtactagat
     3301 cattcactgg aagcaaaaga acaatcactg atctatccag tgaaaaaaaa tgcgaaagat
     3361 gaataccaaa aaggtagttt cagtaaagac aaatttttca cggaagaaga actgcaacta
     3421 ttcatggcgc ataatcggga aaatatgcgg ttagctggag atgcgattac ttccggagaa
     3481 atcaaactaa atccagctta taaagataaa cagcggattg cctgcgaatt ttgtccattc
     3541 agaagcatct gtacgtttga tgtgatgctg aaagaaaata attaccaccg tctggagaaa
     3601 atagataagg aagaagcact gcgtagaata ttgaaaagga gtgaagaaga gtgaatcaaa
     3661 ttcctttaaa accgcaaaat gaacgattca cagatgatca gtggcaagcg atttttgatc
     3721 aaggagataa tctattagtc tctgcgtctg ccggatcagg taaaactaca gtgcttgttc
     3781 gaagagtgat tgaaaaacta aaaatgggtt ttgatatcga tgaattattg attgtgacat
     3841 ttactgaagc tgccgcaaga gaaatgaaag aaagaattca agaggcatta caagaatccg
     3901 tcaatagcga aagtgaccct gtacgtagac agcattttac taagcagtta gttttattgc
     3961 ccacagcgaa tatctcaacc ttacatgctt tttgcctgac agttattcga cgttattatt
     4021 acttaattga tattgatcca gtctttcgca tgttaacaga tgaaacagaa acgatcttga
     4081 tgaaggagga tgtgtgggac gagctgagag aagcacttta tgcagaaaat gatgaacgat
     4141 ttttccaatt aacaatgaat ttttctaatg atcgttcaga tgacggcttg acgaatctgg
     4201 tgttttctct ttatgagttt gcccgtgcca accctgaccc ccaaaaatgg ctagaacagt
     4261 taagcgataa ttatcgtttg cctgaggggc tagcgaaatc tcgattatat caaaaacaaa
     4321 tccgtccttt agttttggca gatatatacc aatgtgtaca gttatatgaa cagatgactc
     4381 agttagcaca aggtgaagga ttagaaaaaa tgcatgagca agtagctgga gaacaacagc
     4441 agatcaagaa tatctatgaa gcgttctcgc aagatcggtt agaagaagct tatgctggtt
     4501 tggaacaact aactttttca acttttaaga gcagtcggaa agcagaattg aaagaaattt
     4561 ccaatgaagt caagggcatg agggataaag caaaaaaact gattcagcag ataagtaaaa
     4621 gttattttcc tgtttcccca agccaaatgg aagaattaac ggacaaagca ctaccgttag
     4681 tcgaagaaat gacaaaagtc acgcaaagct tcatggacgg tttcagtatg cgtaaacggg
     4741 aaaaaggtgt gctggatttc aatgatctgg aacatttagc gttgcagatt ctcacagaaa
     4801 aaacgaaaga tgtatggctg ccaagtgaag cttcaaaaca ttatcgaaaa aaattcaaag
     4861 aagtaatggt agatgaatat caagacgtga accaattaca agaagccatt ttatactggc
     4921 taagagaacc cgatgataca aaaggaaata tgttcatggt tggggatgtc aaacaatcca
     4981 tctattcttt ccgtttagca gatcctagtt tatttatcgg gaaatacgag aatttctcaa
     5041 agaaagaagg cggacgtcga atcgttttgg ctgagaactt ccgctctaga aaagaagtcc
     5101 tttcttttac gaatcttatt tttgaacaac taatggatcc agcagtgggt caaatcaatt
     5161 atgacgaagc agccaaactg atccaaggat tttcagactt tccagagaat gaacagtttg
     5221 agccggaaat catgatctat gaaaaagaac aggaagaatc agagattgag attccgacgg
     5281 atgatatact ggaagacaaa acagaaggcg aactttttat gacaggacta aaaattcgtc
     5341 aattaattga ttcctccttt atgatttatg ataaaaaatc aaaaaaaagt cgtccgatag
     5401 agtacaaaga cattgttctc ttgacaccta caaagaagaa caacttaacg attttagaga
     5461 ttttcaaaac attggatatt ccattagaga tgaatgatgc tcaaaactat tttcaagcga
     5521 cagaaattcg cacgatgatc tcgctgctac aattaatcga taatccctat caagatattc
     5581 cgcttgcggc tgtattgcgc tcaccgatcg tgggtctgat tgaaccagaa ttagccagca
     5641 ttcgattagc taaccgtgca catacttact atgatgctgt gcttgcttat caagcatcaa
     5701 acgaagatga attagcagct aagctggaac attttggcaa gcaactagag cattggcgag
     5761 aacttgctcg ccgttcttcg atcactgact tgttatggga tatctattat gagacgggat
     5821 atctagagta tgtggtcggt ctgccagcag gggcacagag acaagccaat ctttatgcat
     5881 tggttgatcg tgccaaagct tacgagcaaa gttctttcag aggattatac cagttcgtgc
     5941 gtttcattga aaaaatgcaa gaaaaagata aagacttagc agaaccagtc atttcaatag
     6001 aagataatgc tgttcgcgtt atgacgatcc atgcaagtaa aggcttggaa ttcccagttg
     6061 tatttttgtt agatatgaca aaggagttca acttacaaga cttgcgcaat cggtatgctt
     6121 ttgaagaaaa acttggagca ggaattcgct atatggatcc tgaaacacga gtgttatatg
     6181 atacgttacc gtttcaagcc atcaaattgg caaagcaaaa caaattgttg tcggaagaaa
     6241 tgcgtaaatt gtatgtagga ttgactcggg cagaacaaaa gcttttcatt gtcggttcct
     6301 ataaaaataa agagcaaatg atccaaacgt ggtctgaagc agctgatcat gaggagttag
     6361 tttttgatcc tgcgctacgt ttgaagggca gaagcagctt gatgaattgg attggttacg
     6421 gattgatccg tcatcctgaa atgcagaaat acttagagga agaaatttcg acttctcttc
     6481 ttcaacattc aaatgcacag ttttctattt catggatgaa tcagcaatcg atcattgaac
     6541 aaagacagtt gcttgctgaa aaagaactgg ttaacttaga tcagcagatg aaagaagatg
     6601 aaactttatt agcagattcg ttacaaaaac gtctggcata tgaatatcct tatcaagctt
     6661 cttcacaaac cacaagttat caatctgtat ctgaaatcaa acgattattc gaagatccag
     6721 atgatacaca agaatcacgt ttgactctgg aaagcagtca gaacaaagca gcatcccgtc
     6781 aatttcgtta tacgcaagaa caactagcag aaccaaaatt tttgcaaaaa gatcggcaag
     6841 tgtcagcagc tgctgttgga actgctacgc atgctttgtt acaattactg ccattagaga
     6901 tgccaactac agagagtatc catcaaaaat tacaggaatt ggtaaggaaa cggttagtag
     6961 acgaaaaagt cgcgaaaaaa gtagatgttt cttctattat atggttcttt caaacagaac
     7021 ttggtcaaca gctgattgcc aacaaagaaa acgtaaaacg agaacaacct ttttccatgc
     7081 tgcttcctgc tgacgaagta tttcaagact atccaaatca agaagatgaa ctattaatcc
     7141 acggaatcgt cgatggatac ctagaagaaa aagatcatct caatatctat gattttaaaa
     7201 cagattttat tttacatccg gatgatccag cagaaataga tgcaatcgtc caaaaatatc
     7261 aaggacagct aagactttac caacaagcaa tgtcagaagc tttgaataag ccagtagaaa
     7321 atgttttcct aattttgctt cgagtgaaac aaattataaa cattaacaaa taattataat
     7381 tgagcaaact tgacttatgt tttgtaagga ttatgctata ctctgggaag aggtgagtat
     7441 gatggagcta acacagacag aattcgtttt atgccctaaa tttgaaaaaa cattctctat
     7501 tttaggaaaa aaatggaacg gattgattat cgatgtacta ctcgaaaacg gtccgcaacg
     7561 ctttgttgag cttgcggcaa aaatcccgat ggtcagtgat cgagtattag tcgaacgctt
     7621 gaaggaatta gaaaaagaag gaatagtcac tcgaaacttt gcctgtagcg aaaagagccg
     7681 ttcagaatat ctcttgactg aaaaaggagc agacttgcaa aaagcgatgc gtcaaataca
     7741 agtttggagc gaaaaatgga tcacagagga agaatgcggt tgattttgta aaaaaaatct
     7801 tgtaaactat agtccatacg aataagtaaa gaacgatgac gaaaaagagt agtttggcta
     7861 aaccagttca gggagatctg tcgcagactg aaagcagatc ctgtgtttga aaaatgaagt
     7921 tcactttccg agttcgtctg ataaaatcag accggcaatt gtcgttaacg aagtaagagt
     7981 gcggtgtaag tcatttatcc gcaaattagg atggtaacgc gaagacctcg ttccttaaca
     8041 ggaatgaggt ctttttgttt tgtatttgga atttaaagga ggaacaagca catgtctcta
     8101 caagcacaat tagagagctt gaaagaagca acgctcgtca aaatcacagc ggcacaagat
     8161 ttaaatgaat taaatcaaat acgtgttgaa acgttaggaa aaaaaggacc gatcacagaa
     8221 gtattacgtg ggatgaaaga tctaagtcca gaagagcgac caaaagtcgg aagtttcgca
     8281 aatgagatcc gagatctttt gacagaagag atcgtgcgaa aaaaacaagt attagaagaa
     8341 gaagcgttaa atgcagcttt ggcaaaagaa acaatagacg tgacactacc tggtagaaaa
     8401 gtaaaacatg gtacacgtca tatcgtgaca caaatcatgg aagaaattga agatattttt
     8461 gtgggtatgg gctatcagat cgtagaagga tatgaagtag aatctgatca ttacaatttc
     8521 gaacggatga atctgccaaa agatcatcca gcaagggaca tgcaagatac tttctatatt
     8581 tcagacgaga tattgattcg gacacatact tcaccagtcc aagcacggac aatggaaaaa
     8641 catgatttct ctaagggtgc tctacgaatg atctcgccag ggaaagtctt ccgcagagat
     8701 acagatgatg cgacccacag tcatcagttc catcaaattg aagggctagt tgttgataaa
     8761 aacatcacaa tgggcgacct taaaggaaca ttagaagtcg taatgaaaaa aatgtttggg
     8821 gaagatcgtg aaattcgttt gcgtccaagt tatttcccat ttacggaacc atcagtagaa
     8881 gtagatgtca gctgtttcaa atgtggtggt gccggttgta acgtatgtaa atacactgga
     8941 tggatcgaaa ttttaggagc tggcatggta cacccgaatg tgttgaagat gtcaggaatc
     9001 gatccagaag aatattcagg ctttgctttc ggccttggac cagaccgcgt agctatgttg
     9061 cggtatggag tgaatgatat ccgtaatttc tatcaaaatg acttacgttt cttaagtcag
     9121 ttcaaggggg agtaagaaaa tgttagtttc atataaatgg ctcaatcgtt atgtagattt
     9181 atcgaatgta acaccaaaag aattagcgga taagatgtct gtgacaggaa tcgaagtaga
     9241 gggagtaacg gttccagaag aaggcttgaa aaaaatcgtt gtcggtgaag tcaaggaatg
     9301 cgtaccacat ccagattctg atcacctttc tatttgtcaa gtcgatattg gcgaagacga
     9361 actgtctcaa atcgtttgtg gagcgcctaa tgtgaaggtt ggaatcaaag tgattgttgc
     9421 acttcctaac tcacgtatcg ctggtaatgt aaaaatcaaa aaaggaaaaa tgcgtggcca
     9481 agtatcaaat ggcatgattt gctcattaca agaaattggc tattcagata gtgttatacc
     9541 gaaagaatac gctgaaggta tctattatat gccacaagaa gctgtaaatg gcgatccagt
     9601 ttttccttat ttggatatgg atgattcgat tattgaatta tctatcacac cgaatcgtgc
     9661 ggatgcattg tcaatgcgtg gcgtagctta tgaagttggg gcaatttatc gtcagactcc
     9721 tacattcgat gacgaaaagc tggtagaatc ctcacgtcct gctagcgata aaattcaagt
     9781 ggaagttgaa gataaagaag cagttcctgc ttatcaaatc agaatcattg aaggagtaag
     9841 aatccagcca agtcctcaat ggctgcaaaa tcttttgatg aacgaaggca ttcgtccaat
     9901 caacaatgta gtagatgtga cgaactatat tttgctttta ttcggacaac cactacatgc
     9961 atttgactac gacaaattga aatcaaaaga gatctttgtc cgccgtgcaa aagaaaatga
    10021 aacattagta acactagatg gagaatctcg ggaattgaca actgacaatc tcgtcattac
    10081 taacggcaaa gaaccagttg ctttagctgg tgtcatggga gggttagact cagagatcac
    10141 gaatgaaaca acaacagttg cactagaatc tgctttattt gatccaattt cgattcgtcg
    10201 tacagcaaaa caatttaatt tgcgtagtga atcctctgca cgttttgaaa aaggaatcaa
    10261 ccatgcgact gttggagaag ccagtgaggt tgctgcagca atgatcgttt ctcttgcggg
    10321 tggggaagtt ctacaaggcg cggtcaaagg ctcagagatc aaagcagaag acgtatcagt
    10381 gaagatttca cttgaacgca tcaatcaata tttaggaaca tctcttgtcg ttaaagaagt
    10441 aaatgagatt tttgaagcac ttggatttag ctatgaagaa aaggagtatg tttattccgt
    10501 agtagtacca ccaagacgct gggatatcca aatcgaagca gatattatcg aagaagttgc
    10561 tcgtatttat ggatacgacc agttgccatc tacacttcca agtggagaaa cggttgctgg
    10621 aagcttgtca aaagaacaag aaactactcg taaaattcgt acaatctttg aaggaagcgg
    10681 attgagcgaa gcaatcagtt atgcattgac aacagaagaa aaatcaagac agtttgttat
    10741 aaatgaaagt aatattacac gtttggactg gccaatgagc gaagaacgtt ctgtattacg
    10801 aatgaatttg atcagcggac tattagatga tatcagttat aacactgccc gaaaaaatac
    10861 agagattgct ttatatgaaa ttggtcgggt gttcttccaa gaaaatgatc cacgtacaca
    10921 tctgccgcaa gaagtaaaac atgcagcaat cgctatttct ggtatttggg aagaaaaaga
    10981 ttggcagacg aaagctcaaa atgtagactt tttcaccatc aagggtcttt tagaaaactt
    11041 atttgaacaa ctaggcatca ctgaagatgt ccattatcaa acagtgacag aaatgaagga
    11101 aatgcatcct ggaagaactg ctgcgattta ccttggcgaa aaatttattg gttttgtcgg
    11161 tcaagtgcat ccaacgatta caaaaagtta tgatattcca gaaacatatg ttgctgagtt
    11221 tgatttatct gctatagtgg aagctgctca aaaagggatc gttttcgaag cagtgacaaa
    11281 attcccagca gtcagccggg acatcgcttt gcttgtaaaa gaaactgtga caaatcagga
    11341 attaacagaa gttatccgtt ctgctgccgg aagattttta acagatatcc aattatttga
    11401 tgtctatcaa ggagaaaaca ttgaaaaagg acataaatcc atggcgtata gcctgacatt
    11461 cgtcaaccca gaagcaacat tgacagatga agaaatcaat aaagcaatgg aaaaagtcga
    11521 aaaagcgttg gtagaaaacc tagaggcaag tattcgctaa gaaggaaaat atagtaagta
    11581 acgatcaagt agcttttgaa gaccatacat ttgaaaaaaa gatgtgacac aacttcgtca
    11641 catctctttt tttgatttta aaaaaggaat agcacagaga cgagataaaa aaacaggcct
    11701 ttacagcctg cttttttaga gtaaatgaat taatggaagt aattgattcc cattgctgat
    11761 tttacttcag aaagtgtttg tgcagctact ttagcagctt cttcactacc tttacgtaag
    11821 atttccataa cagctgaagg atctttttcc aattcttctc ggcgcgcacg gatcggtgca
    11881 aattcagctt ccaatacatc aatgaggtat cgtttgatct ttacatcacc aagtccgccg
    11941 cggcggtaat gagctttcat ttcttcaatt gcttctttat ctgttccgaa tacatccaag
    12001 taggtgaaga ccatattccc ttctacttgt ccaggatctt gtacatggat gtggtttgga
    12061 tcagtataca tactcatgac ttttttctgt aagacatcgg caggatcgga gatataaata
    12121 ccgtttccta acgatttact cattttgcca tttccatcga ttccaggtaa tctacccatt
    12181 ccttttggtg ggaatacgcc ttttggttcc accaagactt caccatatgt atgattgaag
    12241 ctttgtacga tttcctgtgt ctgctctagc attggttttt gatcctcacc aacaggtaca
    12301 agattcgctt tgaatgcagt gatatctgct gcttgagaaa caggatagat aaagaatcct
    12361 gtaggcacgc tttcaccgaa ttttttttgt tcgatttctg ttttgacagt cggattgcgc
    12421 cgaacgcgtc ctacgctgac taagttcaaa taatacatgg ttaattcagc taattccgga
    12481 atttgtgatt ggataaataa agtagatttt gcaggatcta atcccacggc taaatagtct
    12541 aaagcaactt gcaagacatt tgatgaaacc ttctcaggat ttttagcatt atctgtcagt
    12601 gcttgcatgt cagcaatcat gacaaacagt tggttgtctt catctgcctg catagccact
    12661 cgggttttta gagaaccgac gtaatgccct aaatgcagct tcccagttgg tcgatctccc
    12721 gttaaaataa tatttttcaa aaaagacacc tctttacgat ttgtaagtct gagcacttaa
    12781 tatcttaccg tttttcctga aaagaaacaa cagttcctaa ggaaaaaaca aggaaagagg
    12841 ataaaataac taatagatta gaaatggaag aaatatttat gggagaataa acgttctaag
    12901 aaactttaca aattactaaa ctatcagata taatgtattt tattatacat ctttaaaaga
    12961 aatcttacta aataataatg ggagtgtaat tctatggcaa tgattaaatt tgatcacgtg
    13021 gataaatatt atggcaagtt ccacgcatta aaaaatatca atctcgaatt cgagaaagga
    13081 gaagttgtcg ttgtcattgg tccttctggt tcaggaaaga gcacgatgct tcgctgtatc
    13141 aatggattag agacgatctc ttcaggtaag ctgctgatta atgatactga cttgcatgat
    13201 aaaaagacaa aactgacaga agtccgaaaa aacatcggca tggttttcca acatttcaat
    13261 ttgtacccaa ataaaactgt tctggaaaac atcaaattag caccaatcaa agtgttgaag
    13321 caagaccagg caactgcagt caaaaacgcg gagaaatttt tgaaaactgt caatatgtta
    13381 gataagaaag attcttatcc ttccatgtta tccgggggac aacagcaacg ggtggcaatc
    13441 gccagaggtt tagcgatgaa tcctgagatg ctgctgtttg atgaacctac ttctgctttg
    13501 gatccggaaa tgattggtga tgtattagac gttatgaaaa aattggctca tgacggtatg
    13561 tcaatgattg tcgtgacaca tgaaatgggc tttgccaaag aagtggcgga tcgtgttatc
    13621 ttcatggctg atggagaagt attggaagac agcagaaatg tgagagactt tttcgaaaat
    13681 ccaaatgaag tccgagcaca gcaatttatc agtaaagtta tcaatcattg ataggaggaa
    13741 aaagatgcgc aggttaaaaa aaatacgcct gattttcctt ttaggcattg cttttcttgc
    13801 actagccgga tgtaaaggaa atgggttggc aaatgaagat attttagagc gaagcaaatc
    13861 taccaatgaa atcatctggg gtgtcaagta tgatactcga ttatttggaa tgatggatat
    13921 tgagagcaga actgtacaag gatttgatgt agatattgca aaagcaatca ctaaaaagat
    13981 ccttggtgac aatggaaaaa cagaatttgt tgaagtcact tctaaaactc ggattccatt
    14041 attgaaaaac ggaaatatcg atgcaattat tgcaacgatg acgatcacag atgaacgaaa
    14101 aaaacaggtt gacttttcag atgtctattt cgatgctggc caagctttgt tggtaaagaa
    14161 aggaagccaa atcaaaagtg tagatgattt gaatgcttct acaacggttc tagcagtcaa
    14221 aggttctaca tcggctgcga atatccgaca gcatgcacca gatgcaaaaa ttttagaatt
    14281 agaaaactat gcagaagcat ttactgcttt acaatctggt cagggagacg cgatgactac
    14341 ggataatgcg attttgttag ggattgcaga tgaaaatcca gagtatgagt tagttggtgg
    14401 aacgtttact aatgagcctt acgggattgc gatcaacaaa gggcaagaaa acttcttgaa
    14461 agctgttaac caagcgctag aggaaatgca tgcagatggc acatacgata aaatctatca
    14521 aaaatggttt ccaaatgaaa ctgaaggaaa agtagagtaa gggggagagg caatgagcga
    14581 attgatacaa acttatggac caatctttgt cgacgggttc aagatcacat tgttttcgag
    14641 tgtcttagca cttgtgttta gtttgattat cggaacgtta atggcaatct tccaattatc
    14701 aaagaataaa gtagtgagcg ccttagcaaa ggcatatgta gaatttttta gaaacattcc
    14761 attattaatc atcgtgatgt ttttttatgt tgtcgtaccg atttattggt ttaatctaga
    14821 tggattccaa gcaggtacga ttggattaac gatctataca tctgctttta ttgctgaaac
    14881 agtacgttca gggattcaaa ccgtaccaaa aggtcaaaca gaagcaggac tatcttctgg
    14941 ttttacctat tcagaaatca tgcgttatat tgttttaccg caagcattca aaatcgttat
    15001 cccgccatta ggcaaccaat ttatcaacct cgtaaaaaac tcatctattt tagcaatcgt
    15061 agctggttta gatttgatgt atcaaggaga cttgatcgct agtgaaacat tcaatacctt
    15121 cgatacgtat atcatcgttg ggttatttta cttagttatc acgctaccgt tatcttactt
    15181 aatggtttat cttgagaaaa aatgggcggt tcgttcatag aaggagggga aatacatgga
    15241 ttttagcggc gctttttcat ggatgaatat ccgtttttta ttggaaggat taaaggttac
    15301 tgttgaagtt tcattgtttt cgatcatttt tagtttcttg atcggtggtc tgacaggtgt
    15361 gattcgtttt gcgaatatcc ctttcttttc caaaatcctt ggtgtagtga ttgatattat
    15421 acggaatctt ccattattat tgatcatttt cttcacttac tttgccttac ctcaaatcgg
    15481 cattcagatg aacatcttct ggtccgctgt cgcagcatta acgatttttg agtcagctat
    15541 gttgtcggaa attttccgag ctggactcaa tgctgttcca aaaggacaga tggaagcagg
    15601 attatcaacg ggactgactt atgtagaatc gatgcgaacg atcgtgttac cgcaagcatt
    15661 taaatcaatg atcccggcaa ttgtgagcca attgatttca ctgatcaaag atacttcatt
    15721 agcagtaatc atttctttac ctgaactttc tcatcaggca cgtattattt atggacagaa
    15781 caccaattat gtgcttccga tgttcatcat catgacactt atgtacttcg tggtatgtta
    15841 cgcgctttct ctgctttccg gttacttgga aaagagacgg tacagttatt aatagatgat
    15901 tcttttagcg gttgtggcaa actaaagtca caaccgcttt tcctctttcc attcgaaaaa
    15961 aagagatttc gattgtttta agcaagtaag agcacttgaa aattcacgct aaaaagtaca
    16021 caatagggag gatagaattt attgaaaaaa aggatgatac gattgacaga taatcgccct
    16081 atcggattta ttgattcagg tgtcggcggc ttgactgtag taaaagaagc cctgaaacaa
    16141 ttaccgaatg aaaatatttt atttgtagga gacacagcac gctgcccata tggccctaga
    16201 cccgcggaac aggtaatcca gtatacttgg gaaatgacgg attatctggt ggagcaagga
    16261 atcaagatgc tggtgatcgc ctgcaatacc gcaactgcgg tggctttaga agaaatcaaa
    16321 gctgctcttt ctattccagt catcggtgtg atccttcccg gtactagagc ggcagtaaaa
    16381 aaaacacaaa ataaacaagt tggcattatc ggtacgattg gtacggtaaa aagtcaagct
    16441 tatgaaaaag cactgaaaga gaaagtacca gaattgactg tgacaagtct tgcttgtccc
    16501 aaatttgttt cagttgtcga aagtaatgaa taccattcat cggtggcgaa aaaaattgtg
    16561 gcagaaacat tagctccttt aaccactaaa aaaatcgata cattgatttt gggatgcacc
    16621 cattatccat tattacgccc catcattcaa aatgtaatgg gagaaaatgt tcaactgatc
    16681 gattctggag cagaaacagt aggtgaagta tctatgctgt tagattattt caatttgagc
    16741 aattcaccgc aaaatggtcg gacattatgc cagttttata caactggctc tgccaaactt
    16801 ttcgaggaaa tagctgaaga ctggcttgga atcggacact taaatgtaga acatatcgaa
    16861 ttgggaggaa aataaggatg cgtcatgacg ggagaaaagc ccaagagctt cgcaaactgg
    16921 agcttcaaac aaatgttttc aaatatccag aaggatcggt agtgatccgt tttggtgata
    16981 caacagttat ttgttctgca acaatagaaa attctgttcc cccttttttg agagaaacag
    17041 gaacaggatg ggtgacagca gaatatagta tgctgcctcg agcaacgagt acgagaaatc
    17101 gtagagaaag cagcaaaggg aaactttctg gtagaacaat ggaaatccag cgtcttattg
    17161 gtcgttcctt gcgtgcagta gtagacttgg aaaaattagg agaacgtagt atcatcgtag
    17221 actgcgatgt gatccaagcg gatggcggta ctagaacagc aagtatcact ggggcatttg
    17281 tggcattgaa attagccatt gaaaaacttt taagagaaaa agaattgtca gaagatccaa
    17341 taaaagaaca tcttgctgca gttagcgtag gaatcttacc agatgggact tgcgtcacag
    17401 atttggatta ccaagaagat tccgcagcac ttgtagacat gaatcttgta atgacagaat
    17461 ctggaaaatt cgtggagatt caaggaactg gagaagaagc aacgtttgat ggtgaacagt
    17521 tgaatgaaat gctctttttt ggaaaaaacg cgattgaaga tttgatcaaa gagcaaaaac
    17581 atgctttgct aacagagttt gctcaaaatg atgagcggat agaagaaaca aaaacaatca
    17641 tcattgcgac aagaaatcca ggaaaagcag aagagtttcg aaatatgttc aaagaagcgg
    17701 gatatcatgt aaaaacatta cttgactatc ctgaattgcc agatgtggaa gaaactggca
    17761 gcacatttga agaaaatgcc cgattaaaag cagaaacgat tgcacaattg cttgatcagc
    17821 cagtcttagc agatgattca ggtttgaaag tggatgcttt aggcggtatg ccaggaatct
    17881 attctgcacg ttttgcaggg gaacaaaaaa gtgatgcggg gaataacgcc aaattattat
    17941 atgaattgac agatgtgccg gatgaaaaaa gaacagcaca attccattgt acgttagtat
    18001 tcgcggcacc caaaaaagac agccttgtag tagaagcgga atggcccggt cgagtagccc
    18061 gtataccatc tggcgaaaat ggctttgggt atgatccttt atttatccca gaaggtaaga
    18121 aacaaacagc agcagaatta tcgagcgaag aaaaaaacaa gatcagtcat cgggctcaag
    18181 caatgaaaaa attgagtgcc gagtggaaac aatggctgga aggagagaga tagatgcggt
    18241 atttagtagt aagtgataat catggagaca gagatatcct cgttgactta gtagatcgtt
    18301 atcgttctga agtggatatc tttttccatt gtggtgattc ggaattagaa gcttctgacc
    18361 cattatggga tacattccaa gtggttcaag gtaattgtga ttatggacca ggattcgaac
    18421 agaaaaaagt gatccaaact ggtcaggata cagtattcat gactcacggt catttatcga
    18481 atgtccgatt tggtttgaca cagttagcaa tcgaagctcg agctgcaact gccgatatgg
    18541 tgttttttgg tcatacccat caaataggct gtgagatgga tcagaaaatc ctgtttttga
    18601 atcctggtag catttctcag cctagaggcc cgatccaaat cccatcatat gctcttatcg
    18661 aaagtttaga tgaccaaatt tctgttcaat actataaccg tgcccatcag gcagttgaag
    18721 agttggcctt ttctttttcg agagagcgct gaataaaaaa actctaattt ttaataaaaa
    18781 taagctttat tttatgaatt ttgcatccgg tttcggtaaa atagtagtgt tgaatgaaat
    18841 agaaatggag gccatcagat gattggacct atagtaagag aaatgctgct ggaaaatcag
    18901 gaaacgtttt tgattcctgc agaaaatgtg gcaaatgtta tgtatcatca tccattgtcc
    18961 catggtttac ttgtgttatc gaaggtcggt tattccaaaa ttcctgtatt aggaaaagat
    19021 gatcactttg taggactagt aagtttatca gatgtggtca ataaaatgat tgacttgcaa
    19081 acaatcagca tggacccgtt agaaggacta acggttgctg atgtgacaga aacagatgtt
    19141 ccgacaattg atgaaaattg ggaactagaa gaagtactcc atttgttagt cgattcgcct
    19201 tttttaccgg ttgtcacaga agatcggatt ttcaaaggaa tcattacaag aaaagagctg
    19261 cttaaagccg taaacttcat ggtgcatgaa ttagaacgtc gtaatatatt gtctccgaaa
    19321 atcgaagaat taaaagaaca aatcgatatc gtgagctaaa gaacgagaaa ctaaaagata
    19381 atcaaaaaat aagtagaaaa aacagcgtac cagttttgac tttatcttga aaaagaggct
    19441 gggacaaaag c
//
LOCUS       DAZKLD010000046        18986 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.046, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000046 DAZKLD010000000
VERSION     DAZKLD010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18986)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18986)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18986
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.046"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(7..1467)
                     /locus_tag="ACG8KR_001301"
     CDS             complement(7..1467)
                     /locus_tag="ACG8KR_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0200680.1"
                     /translation="MKIEALLERKEQLQILILRNLVLQGGTASINGLREHVQLSKASF
                     DQYLEDIELIGRMMEKKVEVQRNEYQALLVLDEDVSLEKILLFLLQESLKFKMLVYLL
                     EHQQVSIVRLATAFNISESSVFRKIKELNQLLEEFGLQIKNGQLYGEELQIRYFYYEL
                     FQYIPEDQRPLFLQNTPEKRPFILGLDRVLETTFTASAEAQIACWLGITKKRLLNEKS
                     TYATLKEKKLLYQSDRLYQAIDPIIVMHLSRTAAELNVYETMMFYSFFVSFSIVDEEI
                     FYQYDLTRSKKLPTAVLDTYIRETMLWHYRPRRLKIKEEKAVGYQISQINNEWFFFVG
                     KIEVYERDRLLEQQQKMLGHSLTQLLAKLQETAVQQLPRKRAEDSELSYLMIQYANVL
                     LMIDFYIAKPVVIGIDLESLPIYRIAFQQYLIRELRGIGGIEIGSYEEGKEYDLVITF
                     CQRNKKQCEYYLSEFASPYDIIRLKRRIETLKKEKN"
     gene            complement(1457..2752)
                     /locus_tag="ACG8KR_001302"
     CDS             complement(1457..2752)
                     /locus_tag="ACG8KR_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFQ0200681.1"
                     /translation="MNIVIVGGSFAGIHAAIYLRQLMPTSEIYLIEKQSKLGWIPSGF
                     NLILKGKVTSEKQLSWITKEELTDRYRIHVYTEKTVIGLKEKKVVLAGGKKLDFDRLI
                     LATGSNQKFRNISAESTLIHPVKKIQNVTALQQKIMNAKKIAIIGAGQAGIEIAEGLA
                     SRKKQIHLYESRKSILFRYLDPEMTEPLVKEMKNQGLSLFLEEQVVSLTENETAIVET
                     EKRKENYDLVLLANHSRPDNQMWEEQLTLNDDGTIWVDDYLQTSQKDVYAIGDAIQVT
                     FRPTNEKMYVSLVNNAVRTARNVSKTISGSQTKDQGTYRPVGNQWFGYFLGSVGLTEE
                     ESIFYPQKIETGYVDYKLTAINQSKVKLKFLFDSEKKILGAQLQSKKEMFHLLDQLTI
                     AVEEGWTFEQLEEHELFFQPEYRVPDMVWSKVANTAYED"
     gene            complement(3084..3761)
                     /locus_tag="ACG8KR_001303"
     CDS             complement(3084..3761)
                     /locus_tag="ACG8KR_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019172 - glyoxalase III activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HFQ0200682.1"
                     /translation="MKVLIVLTNVEKYEKIERPTGLWLSELTHFYDVLVKNGIDADFV
                     SPKGGYVPLEPQSVLNMDEIDWAYYEDEDFRNRALGNTLRPDDVDANDYAAVYYAGGH
                     GTMWDFPEAIDLGKVASQIYRNEGLVSAVCHGVVGLLPVTDESGKSILAGRTVTGFSN
                     EEEEANGTTNEVPFLAEDALKEKGANYQSGAAFSEVVQIDGRLLTGQNPQSAHQLGEA
                     VVEALKN"
     gene            complement(3791..4882)
                     /locus_tag="ACG8KR_001304"
     CDS             complement(3791..4882)
                     /locus_tag="ACG8KR_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HFQ0200683.1"
                     /translation="MSQFDQSITLPSGVQLKNRLMLSPMTTQLSFFNGVITEDEITYY
                     KQRSKGLGAVITGAANVQDIGKGWPGELSIAHDEMIPRLSELAKAIQGEGAKAIIQIF
                     HGGRMTSRATLSGEQPVSASAVAAKRPDAETPRAMSEEEIVETIQAFGKATRRAIESG
                     FDGIELHGANTYLIQQFFSPHSNRREDQWGGSLENRYRFIEELLTTVFSAVEQYAKKP
                     FIVGYRFSPEEYETPGIRFEDTIWLLERLRESKLDYLHVSLNMYDRIARSDKYNDKSI
                     LEVVHNTIQGKIPLVGVGGIRNREDVEQVLKYAELAAIGQQMIVDPDWAEKLLENRED
                     EFVTKPFEKAYKELYLPSPLYNFLKARYQ"
     gene            complement(4983..5417)
                     /locus_tag="ACG8KR_001305"
     CDS             complement(4983..5417)
                     /locus_tag="ACG8KR_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFQ0200684.1"
                     /translation="MEKPIEKALDECLFFSVKKLDRMLNKLADEAFRRTGLAPTYGFI
                     LLILKEKDGIPQKDIAQMLYSAPSTIARFVEKLEYKGYVKTVSEGRLSLVYLTEQGRQ
                     FAKEVDGSWEELHQSYKAVLGNEASKELAQTMNDATDKLQKK"
     gene            complement(6001..7458)
                     /locus_tag="ACG8KR_001306"
     CDS             complement(6001..7458)
                     /locus_tag="ACG8KR_001306"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFQ0200685.1"
                     /translation="MKKFPEGFLWGGATAANQCEGAWDVDEKGLSVSDVYTFDSDLPK
                     EKWTDQWHMMTHQQVKAAQDPNSKYYYPKRHGNDFYHHFKEDIRLFAEMGFKCYRMSI
                     AWTRIFPHGDEETPNEAGLFFYDQVFDECLKYGIEPVVSLSHYEMPLYLVTEYGGWPN
                     RKLIQFYVRFAETVFKRYKNKVKYWMTFNEINCVKHHPFVSVGVIEENHPHLEQAKYQ
                     GAHHQFIASALATKACHEIIKGSQVGCMISYQLLVPYSCDPDDIQKTIEQQRTSLFFS
                     DVQARGYYPAYTQRMFEEKGVNLKIEVGDEEILAAYPVDFVSFSYYMSSAVSAHPENW
                     EGVQGNLITGGIKNPYLPSSEWGWQIDPKGLRIALNQLYDRYQKPLFIAENGLGAIDK
                     VEEDGTVQDDYRIDYLKQHIEQMKEAIKDGVDLFGYTSWGCIDIVSASTSQMSKRYGY
                     IYVDQDDEGNGTKKRLKKKSFDWYRQVIQSNGEIL"
     gene            complement(7455..9350)
                     /locus_tag="ACG8KR_001307"
     CDS             complement(7455..9350)
                     /locus_tag="ACG8KR_001307"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFQ0200686.1"
                     /translation="MEKPFSKNYTYLVRRIATALINKGEVKMDYSQLAKDIVRFVGGE
                     ENVSNVYHCATRLRFTLKDNKKADKEKVEQLEGVITVVEAGGMFQVVVGNAVNEVYDV
                     LSKQMKLEDDASSGKRGTEKKGILNSFIDMIAAVFAPTLGVLAGSGLIKGVLALCTSL
                     NLLTTESGTYIILNAAADAFFYFLPIFLAYTAAKKFNTDRFIAMVIAAALVYPTIVSA
                     YSDSITLRFLGMPVILARYTSTVIPAILAVWVLSYIEPKIRKSLHESIRNLLTPFICI
                     IVMVPIILLVVGPIADYASQLIAAGYLAVYNFSPVLSGAVIGGFWQVLVIFGLHWGLV
                     PVMTNNLSFYGRDTLGPACMTAVAAQAGAVLGVFLKTKNKKVKSLSLSAFITALFGIT
                     EPAVYGVTLKYKRPFYIACICGAIFGGVAGAAGAGALAVATRSILSFPIYIGEGFVWL
                     VASYFLAMISSCMLTFLFGYKDEIEEESSKDIVLSTPAAGEIIDLSEVNDPTFASGSL
                     GEGFAIIPTDGKIYSSVNGEVSTVFPTKHAIGVVSEEGAEILIHIGIDTVNLNGKYFQ
                     SAVSDGKKVRKGDLLMEVDLQELIKEGYDPTTMVIVTNSSRFKNIITSHKKNNQTRKL
                     ASLGGSI"
     gene            complement(9408..11183)
                     /locus_tag="ACG8KR_001308"
     CDS             complement(9408..11183)
                     /locus_tag="ACG8KR_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HFQ0200687.1"
                     /translation="MFITKRRCKQMDLNKRQLQILVVLRNKNHWITSEELAEALGTNK
                     KTIQTEIKNMLAIYEKKIIIQSNKRSGYFLESIESETKQQIIQEVTKHKIYSSMDFRA
                     STIVTYLLFQKGYVSMQKIADIFFLSKTAVSLQIKTILRWIERNPKLELEVSSTKGLK
                     IIAEENSKRIFLSLVGTETVIQHARFPQQISTIFSNIMPTVQKTLKEVLISYNYIVSG
                     EDFIRFSRYLVLTILRREELLEKTNRYTLFIPMVETLTKKLSSELPYSFSNQEKQAIE
                     NRLLELNYLMINDEKNKEIEKNLRAFEQTIIRFLDLPVSLLFHETEFLLTHIKQMKTR
                     MDAGHNTMNHFVQKIISRYPLEIYLIRRFFPIHFQLRPNLAESAYFVLYLAEALNPFR
                     KQGEILIVSNQPISILNTLKKQIQQSMNMWIKECRIEPVYLFKNQSNKITEYDAILTT
                     EQELIFEWDDLIYLPSVLDEFESWEIYQILREKLTIVEEKKKTKLLDHYYKEENQIKI
                     HKKIDSIQQLISPSSDLVMLPLNAETLLCCLIKSQVETKITEYQLEIPLIHQQRKIKR
                     VFFVSFDYKRNDILVFFQAVSDLLG"
     gene            complement(11233..12729)
                     /locus_tag="ACG8KR_001309"
     CDS             complement(11233..12729)
                     /locus_tag="ACG8KR_001309"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFQ0200688.1"
                     /translation="MDQEIFPENFLWGGAVAANQCEGAWLEDGKLPNVTDTLIGIMNQ
                     HPSIQWNEEKKIWEIALDESLHYLSHEAIDFYHRFEEDIRLLKELGLKAFRTSISWAR
                     IFPRGDEQKPNEAGLVFYDRLINTLNRYDIEPVITLSHYETPLALVGEYGGWQNRKLI
                     DFFEFYAQTVFERYQGKVKYWMTFNEINNAFRMPYAAAGLVTFPARDKLEPIATLTKK
                     VIYQACHHMFLANARATQLLKKIDPNAKMGIMCSFSALATYAYDCDPENVFGSLQFKR
                     NSWFFSDVMCRGHYPAYIYRTWKEEDCAPVMLDGDEQCLQANTADYIAFSYYRSAVYK
                     KEAEMRVDTGGANGFDNPFLKEKSPEPWSWPIDPLGFRYVMNELTDRYELPLFIVENG
                     IGLDEAPDELNRIHDPKRCEYLKIHLQEIAEAIKDGCQIIGYLWWGPIDIVSAGTGEM
                     RKRYGFIYVDRQNDGSGSFRRLKKDSFEYYKQIIQSNGKNLEYRSFFK"
     gene            complement(12923..14545)
                     /locus_tag="ACG8KR_001310"
     CDS             complement(12923..14545)
                     /locus_tag="ACG8KR_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HFQ0200689.1"
                     /translation="MEQKWWHHSVVYQIYPRSFQDSNQDGIGDLRGIISRLDYLAYLG
                     IDAIWLSPVYESPNDDNGYDISNYQAIMAEFGTMEEMEELIEEAKKRNIRIIMDLVVN
                     HTSDEHPWFIEARKNKENPYRDYYIWRDPVNGKEPNRLRSIFSGSAWQLDEKTGQYFL
                     HLYSKRQPDLNWKNKQVRQEIYQMMNFWIDKGIGGFRMDVIDMIGKEPDKEITSNGPK
                     LHEYLQEMNQATFGDKNLMTVGETWGATTEIAKLYSNPKRNELSMVFQFEHIGLDQQE
                     GKDKWDLKPLSISELKKVLSKWQTSLGDEGWNSLFWNNHDLPRIVSRWGNDDVYRKES
                     AKMFAILLHMMKGTPYIYQGEELGMTNCPIDDIAEAKDIETINMYNERISSGFTKEEI
                     LESIKAKGRDNARTPMQWNAREHAGFTTGIPWLRVNPNYKEINAEEALADQDSVFYMY
                     KKLIDLRRKHDIIVWGDYELVEKTPEHVFAYSRIYEDQQWLVICNMSSEKQTFTIPRE
                     ALRVIVSNYPLKQLPDGKVDLRPYEAFVLEVK"
     gene            complement(14873..16186)
                     /locus_tag="ACG8KR_001311"
     CDS             complement(14873..16186)
                     /locus_tag="ACG8KR_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HFQ0200690.1"
                     /translation="MKEKILSYFEIEKLNTTVKREVLAGFTTFISMAYILFVNPTVLG
                     ASGMDEGAVFTATALASAIGCVLMGVLARYPIGTAPALGINAFFAYSVCLGMGIPWET
                     ALAGVFVASLIFILITIFKLREMIIDAIPADLKYAISGGIGLFIAFLGLSEGGIIVSN
                     ESTLVGLGSLNVGSTWLTIFGLVVTAILLVRRVPGGIFIGMAATTILGLVTGLIPMPS
                     EIVAAAPSLKPTFLVALKHVGDINSLQMWVVVLTFLLVTFFDTAGTLVGLANQAGFMK
                     DNKMPRVGKALASDSTAMLAGSLFGTSPVGAFVESSAGIAVGGRSGITAITTGIFFLL
                     GLFFSPLLSVVTSQVTAPALIMVGVLMAQSLRQIEWGNLEIAIPSFLILIGMPLTYSI
                     SDGIALGFIFYPITMLAAKRGKEVSPIMYGLFFVFVGFMWILNVK"
     gene            complement(16431..16655)
                     /locus_tag="ACG8KR_001312"
     CDS             complement(16431..16655)
                     /locus_tag="ACG8KR_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0200691.1"
                     /translation="MDLMGRLEGYLKYRRAKHSEKLRAKNLKSGKKKTKGWGKMVDTG
                     FGGLNNLNPVDTKEFIKEEQKRNGKIKSHY"
     gene            complement(16675..17097)
                     /locus_tag="ACG8KR_001313"
     CDS             complement(16675..17097)
                     /locus_tag="ACG8KR_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFQ0200692.1"
                     /translation="MESVYRQERKSNRKKGQMMIQKTRVMLYVNDVEMICRFFVEKIG
                     AEISETIELPEEFKSIVLSISKELELGVFPKVFVQKFSPEVLGPPPSLVFFTDEFETI
                     YENMEEPGEITDNNGILTFNFSDPEGNYFVIGKAESSD"
     gene            complement(17239..17949)
                     /locus_tag="ACG8KR_001314"
     CDS             complement(17239..17949)
                     /locus_tag="ACG8KR_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase beta subunit-related
                     protein"
                     /protein_id="HFQ0200693.1"
                     /translation="MKKVIVDTNFWEVFPEATIEILSVNGIDNHVTKESEETYHQLLN
                     EAAKEARNYLTEETFSQNEVIAQWRQAFTTFKTKKGARSSIEALLKRANQEREFFPIN
                     PLVDIYNSISLSFGVPCGGEDIEKIAGNLHLGKAQGGESFLPLGADSDAPALPEEIIY
                     YDEQGAICRCLNWREAQRTMLTEETQAAVLVIESINAEQAARGAQAIEALKEKIDQTF
                     GVTSVRHTLTKDESEAEL"
     gene            complement(17967..18464)
                     /locus_tag="ACG8KR_001315"
     CDS             complement(17967..18464)
                     /locus_tag="ACG8KR_001315"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0200694.1"
                     /translation="MTPSDVDLLQQISIETFTDTFGKDNSPEHLADYLTQAYALEKLT
                     EELKDKETDFFLVSIDQEPAGYLKLNTGTSQSEEMADHFLEIERIYVRKKFKHQGIGT
                     FLLDTAIEKAKEKQKTAIWLGVWEHNDVALAFYRKKGFIKYSEHVFYMGDDPQTDYLF
                     IKQLF"
     gene            complement(18501..18953)
                     /locus_tag="ACG8KR_001316"
     CDS             complement(18501..18953)
                     /locus_tag="ACG8KR_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFQ0200695.1"
                     /translation="MAEILRDIGMIARALDSISNIEFKEVDLTRGQYLYLVRICENPG
                     IIQEKLAEMIKVDRTTTARAIKKLESNGMIERLEDKENKKIKKLYPTKKGAEIYPFII
                     RENNYSNAVALNGLSDEEAKQLEYLLNKVCKNVSEDWNFVKKGNKRIY"
BASE COUNT         5496 a         4028 c         3079 g         6383 t
ORIGIN      
        1 ttttaatcaa ttcttttctt ttttcagcgt ctctattctt cttttcaatc gaataatgtc
       61 gtaaggtgac gcaaattctg acagataata ttcacactgt tttttgtttc tctggcaaaa
      121 tgtgatcacc aaatcatatt ctttaccttc ttcataactt ccaatctcaa taccgccgat
      181 tcccctcagt tctctgatca gatattgttg aaaagcaata cgataaatcg gcagactttc
      241 taaatcgatt ccaatcacta ctggttttgc aatataaaaa tcgatcatca aaagaacatt
      301 ggcatactgg atcatcaggt aactaagctc actatcttca gctcttttcc tcggcaattg
      361 ctgcaccgct gtttcctgta atttcgccag taactgtgtt aaggaatgcc cgagcatttt
      421 ctgctgctgt tccaaaagcc gatcacgctc atagacttcg atctttccaa caaagaaaaa
      481 ccattcattg ttgatctggg aaatttggta gcctactgcc ttttcttctt tgattttcaa
      541 acgacgagga cgataatgcc ataacatcgt ttcccgaatg taagtatcta aaacagcggt
      601 tggtaacttt ttgctccgtg tcaaatcata ctgataaaat atttcttcgt ctactatcga
      661 aaaagaaaca aaaaagctgt aaaacatcat tgtttcgtat acattcagtt ctgctgctgt
      721 tcgactaaga tgcatcacga tgattggatc gattgcttga taaagccggt ccgactgata
      781 gagtaatttc ttttctttca aagttgcgta cgtacttttt tcattcaata gtcttttttt
      841 cgtaatcccg agccagcaag caatctgtgc ttctgctgat gccgtaaacg ttgtttctaa
      901 tacacggtcc aatcctagaa tgaacggtct tttttctggt gtattttgca aaaaaagcgg
      961 tcgctgatct tccgggatat attggaatag ttcataataa aaatatcgta tctgtagctc
     1021 ttctccgtat aattgaccgt ttttgatttg taatccaaat tcctctaaga gctggttcaa
     1081 ttctttgatc tttcgaaaaa cagaagactc actgatatta aacgctgtcg ctaatcgaac
     1141 gatggatact tgctgatgtt ctaataaata gacgagcatc ttaaacttca agctttcctg
     1201 caataaaaac aatagaattt tttccaaact tacatcttcg tccaacacaa gcagcgcctg
     1261 atactcattt cgctgtactt ctactttttt ttccatcatt cggccaatca attcaatatc
     1321 ttccaaatat tgatcaaacg aggcttttga cagttggaca tgctctctca aaccattgat
     1381 agaggctgtt cccccttgca gaacaaggtt tcgcaaaatc aggatctgta actgctcttt
     1441 tctttctaat aatgcttcaa tcttcatagg cagtattcgc cacctttgac cacaccatat
     1501 ctggcacacg gtattctggc tggaaaaata gctcatgttc ttctaactgt tcaaacgtcc
     1561 atccttcttc tactgcaatt gtcagttggt caagaaggtg gaacatctct tttttgcttt
     1621 ggagctgtgc tccaagaatc tttttttcag aatcaaatag aaatttgagt ttaactttgc
     1681 tttgatttat tgctgtaagc ttgtagtcta cgtatcctgt ttcaattttt tggggataaa
     1741 aaatgctctc ttcttctgtc aaacctacgc ttcctaagaa atagccaaac cactgattgc
     1801 cgactggacg ataggttccc tgatcctttg tctgtgagcc actaattgtc ttgctgacat
     1861 ttcgtgctgt acggacagcg ttatttacta gagaaacata cattttttca tttgttggac
     1921 gaaaagttac ttggatcgca tcgccaatcg cataaacgtc tttttgagac gtttgcaagt
     1981 agtcatctac ccagatcgtt ccatcatcat tcaatgttaa ttgttcttcc cacatctgat
     2041 tgtcgggacg agaatgatta gccagcaaaa caagatcata gttctccttt cttttttccg
     2101 tctctacaat agcggtttca ttttcggtca aagacacaac ttgttcttct aaaaaaagtg
     2161 aaagcccttg atttttcatt tcctttacta acggttctgt catttcagga tctagataac
     2221 gaaaaagaat tgactttcgg ctttcgtaaa gatggatttg tttttttctt gatgccaacc
     2281 cttcagctat ttcgattcct gcttgtcctg caccaataat tgcaattttc tttgcattca
     2341 tgatcttttg ctgaagagcc gttacgtttt gtatcttttt aactgggtga ataagcgttg
     2401 attctgcaga aatatttcga aacttttgat ttgaaccagt cgctagaatc aaacgatcaa
     2461 aatccaattt ttttcctcct gctaaaacga cttttttttc tttcaatcct attactgtct
     2521 tttctgtgta aacatggatt cgatagcggt cagtcagttc ttctttcgtg atccaagaaa
     2581 gttgtttttc actggtaacc tttcctttta atatcaaatt gaacccgctg ggaatccatc
     2641 ctaacttcga ttgcttttca atcaaatata tttcacttgt tggcatcaat tggcgaagat
     2701 aaatagccgc atgaatccct gcaaacgacc cgccgacaat tacaatgttc atctgcattc
     2761 ctctttctta taaggtgttc tactgctaac ttctcaatca gaaaacacag tattcataac
     2821 tcgctttcaa ccttatgata catagcgatg cagcagaatc aaccctctcc atcataccgt
     2881 gttctaggtc tgcttctcaa taatgtacta gtggacctaa ttgaagagta tcatttctga
     2941 agagatgttt caaaaaatgt actcttataa aagaaaaaaa ctcgcctttt acgtacctct
     3001 gtccaaatag gaaacagaaa agaacgaaaa gacgagtttt agcgtgtgta atgtaatagt
     3061 tagaatagac acatcattta tttttaattc tttaatgctt caacaactgc ttcgcctaat
     3121 tgatgagcag attgaggatt ttgccctgta agaagccgcc catcaatttg aaccacttct
     3181 gaaaaagctg ctccagactg atagtttgct cctttttctt tcaaagcatc ttctgctaag
     3241 aatggtactt cattcgttgt accatttgct tcctcttctt cattactgaa cccagtcact
     3301 gtacgtccag ctaaaataga ttttccagac tcatctgtaa ctggcaacaa tcctacaaca
     3361 ccgtgacaaa cagctgagac cagcccttca tttcgataga tttgtgaagc gactttccca
     3421 agatctattg cttctggaaa atcccacatt gttccgtggc caccagcata ataaaccgct
     3481 gcataatcat ttgcatccac atcatcagga cgtaaggtat ttcccaaggc ccgatttctg
     3541 aaatcttcat cttcataata ggcccaatcg atttcatcca tattcaaaac actttgcggt
     3601 tctaaaggta catatccgcc tttaggactg acaaaatctg catcgatccc attttttact
     3661 aatacatcat aaaaatgagt cagttcactt aaccacaaac ctgtaggacg ctcgatcttt
     3721 tcgtactttt ctacatttgt taatacgatc aatactttca ttttcccatc ctctttctat
     3781 aaaaattgcg ttattgataa cgtgctttca agaaattata taatgggctt ggcaaataaa
     3841 gctctttata tgctttttca aaaggtttcg ttacaaattc atcctcacgg ttttccaata
     3901 atttctctgc ccagtcagga tcgacgatca tttgctgtcc aattgctgca agttctgcat
     3961 atttcaatac ctgctctaca tcttcgcgat tgcggatccc accgacacca actaaaggga
     4021 tctttccttg aatcgtgtta tggaccactt caagaatcga tttatcgttg tatttatcag
     4081 aacgagctat tcgatcatac atatttaatg aaacatgcag ataatctagt tttgactcac
     4141 gtaatcgttc aagaagccag atcgtgtctt caaaacggat acctggtgtt tcatattctt
     4201 ctggtgagaa acgataccct acgatgaatg gttttttagc atattgttca accgctgaga
     4261 aaaccgttgt taataattct tcaataaaac gataacgatt ttccaagcta ccgccccatt
     4321 gatcctctcg tctattcgaa tgcggagaga aaaattgttg gatcaaatac gtatttgctc
     4381 catgcaactc gattccatca aatcctgatt caatcgctct acgtgtggct ttgccaaatg
     4441 cttggattgt ttcaacgatt tcttcttcac tcattgctcg tggtgtttcc gcatcaggac
     4501 gttttgctgc aactgcactt gcagatacag gctgttctcc agataaagtt gctcgacttg
     4561 tcattcgtcc accgtggaaa atctgaataa tcgcttttgc gccttctcct tggattgctt
     4621 tcgctagctc acttaatcga ggaatcattt catcatgagc gatgctgagt tcacctggcc
     4681 agccttttcc gatatcttgg acatttgcag caccagtaat aacagcacca agacctttag
     4741 aacgttgttt ataataagtg atctcatctt cggtgatcac tccattgaaa aagctgagtt
     4801 gtgtcgtcat aggcgacagc atcaatcggt tcttcaattg aactcctgaa ggtaaagtaa
     4861 ttgattgatc aaattgtgac attgtcattc ctcccataat tcgctctgat taacctatcg
     4921 agaatcatct atcattaaac cactcgatta gttgtacatg caactaattt gcttaaaaaa
     4981 ttttattttt tttgtaactt atctgttgca tcattcattg tctgggctaa ttctttactt
     5041 gcttcatttc ccaaaactgc tttgtatgac tgatgaagtt cttcccagct accatcgact
     5101 tccttagcaa attggcgtcc ttgttctgta agataaacta atgacaagcg accttcgcta
     5161 actgttttga cgtatccttt atattctaat ttttcaacaa aacgagcaat cgttgatggg
     5221 gcggaataga gcatttgtgc aatatctttt tgtggtattc catctttttc ttttaatatc
     5281 aataaaataa aaccataagt tggtgccaga cctgtccttc taaacgcttc atcagctaat
     5341 ttattcaaca tgcgatctag ctttttcacg ctaaagaaaa gacattcatc taacgctttt
     5401 tcaattggct tttccatttc atcccctcca ttggcgtaca tatcatagta tgatttttat
     5461 tttttgtcaa aaaacacgga ataatgtccg cctcctctgt ctagatttga aaaaaagaag
     5521 tagccatctt caaagaattt cggcttgttt tcgacaaaac tccttctgga acaccgtgaa
     5581 tcagaggttg tgaaaaagtc gtttagtttt gagtatgaag aaaaattttg tgaaaatggc
     5641 tttccacatt tttgcataaa tttggcttaa taccgaaaaa ctggcttttg aacaccgttt
     5701 atatcagagg ttgtgaaaaa gccgtcctgc atcaagtaat aagaacagcc aaacaaaaat
     5761 agttcctcat attttgtcgt tttttgagct aatgccgatg gtcagctgat tgtacaccgt
     5821 ttattgagag gtcgtgagcc tgccgttgaa ctccgagtca caaggagcaa tttcaaaaaa
     5881 cagcttctca tgttttatga ctatggtgac ttgtgatcga ggagttggca ggagaatact
     5941 gtttattcgt aaagaagggg ctgtgacaag acttttgtca cagtccctta tttcctttta
     6001 ttataggatt tcgccatttg attggatgac ttgtctgtac caatcaaatg attttttctt
     6061 taaccgtttc tttgtgccat tcccttcgtc atcttgatca acatagatat atccataacg
     6121 ttttgacatt tgtgaagtag aagcgctgac gatatcaatg cagccccacg acgtataacc
     6181 aaataaatct actccatctt tgatagcttc cttcatctgc tcaatatgct gcttcaaata
     6241 gtcaatgcgg taatcgtctt gaacggttcc atcttcttct actttgtcta tagctccgag
     6301 tccgttttca gctatgaaca gtggtttttg atacctatca tataattgat tcaaagcaat
     6361 tctcaaaccc ttaggatcaa tctgccagcc ccattcactt gatggaagat acggattttt
     6421 aattcctcca gttattaagt ttccttggac cccttcccaa ttttctggat gagcactgac
     6481 tgcactagac atgtagtagc taaaagaaac aaaatcaaca gggtaggctg ctaagatttc
     6541 ttcatcacct acttctattt ttaagttgac acctttttct tcaaacattc gttgcgtata
     6601 cgctggataa tatcctcttg cttgaacatc actaaagaac aaagaagttc tttgctgctc
     6661 aattgttttt tgaatatcat ccggatcaca agaatacgga acaagaagct gatagctgat
     6721 catacaaccg acttgtgaac ctttgataat ttcatgacaa gcttttgtag ctaaagcact
     6781 tgctataaac tgatggtgtg caccttggta tttcgcttgt tccaaatggg gatggttttc
     6841 ttcaattact cctacgctaa caaaagggtg atgttttaca cagttgatct cattgaaagt
     6901 catccaatat tttactttat ttttatagcg tttgaatact gtttcagcga aacgaacgta
     6961 aaactgaata agctttcgat taggccatcc accatattct gtaaccaaat ataacggcat
     7021 ttcataatgg gaaagagaaa cgactggctc aatcccatat tttagacatt cgtcaaacac
     7081 ttgatcataa aaaaataatc ccgcttcatt gggtgtttct tcatcaccat gaggaaaaat
     7141 tcttgtccat gcgatagaca tccgatagca tttaaaaccc atttcagcaa ataaacggat
     7201 gtcttcttta aagtgatgat agaaatcatt tccgtgtctt ttaggataat aatacttgct
     7261 gttgggatcc tgcgccgctt ttacttgctg atgcgtcatc atatgccatt gatctgtcca
     7321 tttttcttta ggtaaatcgc tgtcgaatgt atacacatca gacacagata atcctttttc
     7381 atctacatcc catgcacctt cacattgatt ggccgctgtc gctccgcccc ataaaaatcc
     7441 ttctggaaat tttttcatat cgatccccct aaacttgcta gttttcttgt ttgattattt
     7501 ttcttatgac tagtaatgat atttttgaat ctactactat ttgtaacaat aaccattgtg
     7561 gtcggatcat atccctcttt gattaattct tgcaaatcta cttccatcaa taaatcgcct
     7621 ttacgtactt tttttccatc agatactgca ctttgaaagt atttgccatt taaattcact
     7681 gtatcaatac cgatatggat cagtatctct gctccttctt cactgactac cccaattgca
     7741 tgtttcgttg gaaaaactgt tgaaacttcc ccgtttacag aactgtagat ttttccgtcc
     7801 gttggaatga tcgcaaaccc ttctcctaaa gatccactag caaatgttgg atcgtttact
     7861 tcagataaat caattatttc acctgctgca ggagtactta acactatatc ctttgaggat
     7921 tcttcttcga tttcatcctt gtagccgaaa agaaaagtta gcatacagga actgatcatc
     7981 gctaagaaat aactagcaac tagccaaaca aatccttcac caatataaat cgggaaagac
     8041 aaaatacttc ttgtagcaac tgccaaagca ccggctcctg cggcaccagc tactccacca
     8101 aatatagctc cacatataca agcaatgtaa aatggtcgtt tatatttaag agtcacacca
     8161 taaacagcag gttcagtgat tccaaataat gctgtgataa atgcagataa agataaactt
     8221 tttactttct tatttttcgt tttcaaaaat accccaagta ctgctcctgc ttgcgcagct
     8281 acggcagtca tacatgctgg acctagcgta tcacgaccgt agaaagaaag attgttggtc
     8341 attactggta caagtcccca atgtaaccca aaaatcacta acacttgcca aaatccgccg
     8401 ataacagcac cagataaaac agggctaaag ttataaacag ctaaatatcc agctgcaatc
     8461 agctgacttg cataatctgc aattggacct acaactaata aaataattgg gaccatcacg
     8521 atgatacaaa tgaatggtgt caataaattt cgaatacttt cgtgcaagct tttgcgaatc
     8581 ttaggctcaa tataagaaag aacccataca gctaaaattg ctggaataac cgtacttgta
     8641 taccttgcta gaatcacggg catccctaga aatcgcaagg ttatgctatc actatatgca
     8701 ctgacaatcg taggataaac gagagctgct gcaataacca tagcgatgaa acgatctgta
     8761 ttgaactttt ttgcagcagt atatgccaaa aatataggca agaaatagaa aaatgcatca
     8821 gctgctgcat tcaaaatgat ataagttcct gattcagtag ttaataaatt caaactcgta
     8881 cataaggcga gtactccttt gatcaaacca cttcctgcca agacgcctaa agtcggggca
     8941 aaaacagcgg cgatcatgtc tataaaagag tttaaaatgc cttttttctc agttcctctc
     9001 tttccactag atgcatcatc ttctagtttc atttgtttgg ataatacgtc atacacttca
     9061 tttactgcat ttcctactac gacttgaaac attcctccag cttcgactac agtgatcaca
     9121 ccttctagtt gctctacctt ctccttatct gccttcttat tatcttttag ggtaaagcgt
     9181 aaccttgttg cacaatggta aacattgctg acattttctt cgcctccgac aaatcggaca
     9241 atatctttcg ctaattgtga atagtccatt tttacttccc ccttgttaat taatgccgta
     9301 gcaatccttc ttacaagata agtatagttc ttagaaaacg gtttctccat tcaattagta
     9361 ccccttctaa tagcgaacga aactgtacaa tagtgtgtat ggtttactta gccgagcaaa
     9421 tcactcactg cttgaaagaa aacaagtata tcgtttctct tatagtcaaa ggagacaaaa
     9481 aaaacacgct tgatcttcct ttgctgatgt ataagtggta tctcaagctg atactctgtg
     9541 atttttgttt ctacttggct tttgataaga cagcacaaca aagtctcggc atttaacgga
     9601 agcataacta ggtcagaact aggagagatt agttgctgaa tggaatctat ctttttatgg
     9661 atttttattt gattttcttc tttgtagtaa tgatcaagca gttttgtttt ctttttttct
     9721 tcaactatgg tcagcttttc acgcaaaatc tgataaatct cccaagattc aaactcatct
     9781 agaacactag gtagataaat caaatcatcc cactcaaaaa taagctcttg ctcagtagtc
     9841 aaaattgcat catattccgt tattttgttt gattgatttt tgaaaagata cactggttct
     9901 atccggcatt ctttgatcca catgttcata ctttgttgaa tctgtttttt tagtgtattc
     9961 aaaatactaa ttggctggtt actaacaatc agtatctccc cttgttttcg aaaaggattc
    10021 aacgcttcgg caagatacaa aacaaaataa gcgctttcag ctaaattggg acgtaattga
    10081 aaatgtattg ggaagaaacg ccgaattaaa taaatttcta aaggataacg ggagatgatc
    10141 ttttgtacaa aatgattcat ggtattatgt ccggcatcca tcctcgtttt catttgtttg
    10201 atatgggtta gcaaaaattc tgtttcatgg aacaataacg aaacaggaag atctaaaaaa
    10261 cgaataatcg tttgctcaaa tgctcttaga ttcttctcta tttctttgtt tttttcatca
    10321 ttaatcatca aataatttaa ctctaatagt cgattttcaa ttgcttgttt ttcttgattg
    10381 gaaaaagaat agggtagttc tgatgataac ttttttgtga gggtctctac catcggaata
    10441 aataatgtat acctgtttgt tttttctaaa agttcttctc ttcgaagaat ggtcaaaacg
    10501 agataccgag aaaatcgtat gaagtcctct cctgaaacaa tataattata tgatataaga
    10561 acctctttta atgtcttttg gacagtcggc atgatattgg aaaaaattgt actgatttgt
    10621 tgaggaaacc ttgcgtgttg gatcaccgtt tctgttccaa caagagataa gaaaattctt
    10681 tttgaatttt cttcagcaat gatttttagt ccttttgtcg acgaaacttc cagttctaat
    10741 ttaggattgc gctcgatcca acgcaaaatc gtttttattt gtaatgatac ggctgttttt
    10801 gaaagaaaga aaatgtctgc tatcttttgc atactgacat accctttttg aaacaataag
    10861 tacgtcacaa tagttgaagc tcggaaatcc atgctggaat agattttatg tttcgtcacc
    10921 tcttgtatga tttgctgttt agtttcagat tcaattgatt ctaaaaaata acctgaacgt
    10981 ttatttgact ggataatgat tttcttctcg tagatagcaa gcatattttt gatttcagtt
    11041 tggatcgttt tcttgttggt acctaaagct tcagctaatt cttcgcttgt tatccaatga
    11101 ttcttatttc ttaatactac aagaatttgg agttgtcgtt tatttaagtc catttgctta
    11161 cacctcctct ttgttataaa catacaggaa aaacctctca aatgacaata gtcacttgca
    11221 gaggtttaaa aatcatttga agaaagatct atattcaagg tttttaccat tagactggat
    11281 aatttgttta taatattcaa aactatcttt tttcaatctc ctaaagcttc cgctcccatc
    11341 attttgtcta tccacataaa taaagccata tcgcttcctc atttcccctg ttcctgccga
    11401 tacgatatcg attggtcccc accataagta accaatgatt tggcaaccat cttttattgc
    11461 ctcagcaatt tcctgaagat gtatttttag atactcacaa cgttttggat cgtggattcg
    11521 attcaattca tctggtgctt catctagtcc aatgccattt tcaactatga ataatggtaa
    11581 ttcataacgg tctgtcaatt cattcatgac ataacgaaac cctagaggat caattggcca
    11641 actccaaggt tcgggagatt tttccttcaa aaagggatta tcgaaaccat ttgctccgcc
    11701 agtatctacg cgcatttctg cttctttttt atagacagcg ctacgataat aactaaatgc
    11761 aatataatct gccgtgttag cttgcaagca ctgttcgtct ccatctaaca ttactggtgc
    11821 acagtcttct tccttccatg tcctgtaaat ataagctgga taatgccctc ggcacatgac
    11881 atcggagaaa aaccatgaat tgcgtttaaa ttgcagtgaa ccaaatacat tctctggatc
    11941 acagtcatat gcataagtcg ctaatgcaga aaagctacac ataataccca tcttagcatt
    12001 tggatcgatt ttctttaata attgagtagc acgggcgttc gctagaaaca tatgatgaca
    12061 ggcttgataa atcacttttt ttgttagtgt agcaatgggt tcaagtttat ctctagcagg
    12121 gaatgtaacc aatccagcag ctgcataagg catacgaaaa gcattattga tctcattaaa
    12181 ggtcatccaa tatttcactt tcccttggta gcgttcaaag acggtctgcg cataaaattc
    12241 aaaaaagtcg attaattttc gattttgcca ccccccatac tctcctacca atgctaaggg
    12301 ggtttcataa tgactaagag taattactgg ctcgatatca tatcgattta atgtattgat
    12361 tagtctgtca taaaatacga gaccagcctc atttggcttc tgttcgtctc ctcttggaaa
    12421 tattcgtgcc catgagatgc ttgttcgaaa agcttttaaa cctaactcct tcagtaaacg
    12481 aatatcctct tcaaatcgat gataaaaatc aatcgcttca tgagaaagat aatggagtga
    12541 ttcgtctaaa gcaatctccc aaatcttttt ttcttcattc cactgaatac taggatgctg
    12601 gttcatgatc ccgataagcg tatctgtcac attaggtaat ttgccgtctt ccaaccatgc
    12661 accttcacat tgattagcag ctactgctcc gccccataaa aaattttctg gaaatatttc
    12721 ctgatccata actatctctc ctcgctatca acaatttctt acttgaagta tagctcgatt
    12781 catcaaaaca agatattcta actatttcgg cagaagagaa gaaaaaaatt gaatgctact
    12841 cttcattttt aatatttata tataacaaat acataagacg atccccgaaa gaaaacactc
    12901 gttaaccatc taagcaaacg atttatttta cttccaacac aaatgcttca taaggtcgta
    12961 aatcaacttt gccgtctggt aactgtttca gaggataatt tgacacaata acccttaaag
    13021 cttcacgtgg aatagtgaac gtctgctttt cactggacat attacaaata acgagccatt
    13081 gctggtcttc atatatgcga gaataggcaa agacatgctc aggcgtcttt tccactaatt
    13141 catagtctcc ccagacgatg atgtcatgtt ttctacgcaa atcgatcaat ttcttgtaca
    13201 tataaaaaac agaatcttga tctgctaacg cttcttctgc attgatttct ttataatttg
    13261 gattgactcg aagccatgga attccagtag taaacccagc atgttcacgg gcgttccatt
    13321 gcataggcgt cctagcattg tctcttcctt tcgccttgat tgattccaag atttcctctt
    13381 ttgtaaatcc agaagaaatc ctttcgttat acatattgat tgtctcaata tcttttgctt
    13441 ccgcgatatc atcgattggg caattcgtca tgcctaactc ttctccttga tagatataag
    13501 gggtgccttt catcatatgg agcaagatag caaacatctt ggcactttct tttcgataca
    13561 catcgtcatt tccccaacga gaaacaattc gcggaagatc gtgattattc caaaataaac
    13621 tattccatcc ttcatcacct aaagatgttt gccatttgga taatactttt ttcagttcag
    13681 agatactcaa gggtttcaaa tcccatttgt ctttgccttc ttgctgatct aatcctatgt
    13741 gttcgaattg gaagaccatg gataattcat ttcttttagg attggaataa agtttagcga
    13801 tttcggtagt tgctccccat gtttcgccaa cagtcatcag atttttgtca ccaaaagttg
    13861 cttgattcat ttcctgaaga tactcatgca gtttaggtcc attacttgtg atttctttat
    13921 ccggttcttt gccaatcata tcgatcacgt ccatacgaaa gcccccaata cctttatcga
    13981 tccagaaatt catcatttga taaatctctt ggcgaacctg tttatttttc caattcaaat
    14041 caggttgacg cttgctatac aagtgcagaa aatattgccc cgttttttca tccaactgcc
    14101 aagcagaacc actgaatatt gagcgcaatc tattaggctc ttttccattg accggatcac
    14161 gccaaatata atagtcccga taaggatttt ctttattttt acgggcctca atgaaccacg
    14221 gatgttcatc agaagtatga ttcaccacca gatccatgat gatacggata tttcttttct
    14281 ttgcttcttc aatcagttct tccatttctt ccatcgtacc gaattctgcc atgattgctt
    14341 gataattact gatatcatac ccattgtcat cattcggaga ctcgtatact ggagaaagcc
    14401 aaattgcatc aatacctaaa tacgccagat aatccaacct ggaaataatc ccccgcaaat
    14461 ctccaatacc atcttgattt gaatcttgaa aacttcttgg atagatttga taaacaactg
    14521 aatggtgcca ccatttttgt tccatgattt tcctcctata aatggatgtt tctatttttt
    14581 gcttaatcat ttattttagt ctctctcact aaaaattgta acgctttcaa atagctattg
    14641 caccttgtta tcggtaacag aataaaacaa aaaaacagct gaggaaattc cctcaactgt
    14701 tttttgaaag gattgattta tgcagggaag aatgctgatt tcacaacttc tttataaacg
    14761 gtgttcactc agctgtccct tggtattagc tcaaaaaacg gcaaaatata agaaactatt
    14821 ttttcgtttt tttctctaat actcaggcca gaacgctgat ttcacaacct ctttatttca
    14881 cattcaatat ccacatgaat cccacaaata caaagaacag accatacatg attggagata
    14941 cttcttttcc tcttttagct gctaacatgg taattggata gaagataaaa cctaatgcaa
    15001 tcccatctga aatactataa gtcaaaggca tgccgatcaa gatcaagaat gaagggatcg
    15061 caatctctaa gtttccccat tcgatttgtc gcaaagattg tgccatcaat actcctacca
    15121 taatcaatgc tggtgcagtt acttgtgaag taacaactga tagtaatgga gaaaagaaca
    15181 agcctaacaa gaagaaaatc cctgtagtaa tagctgtgat acctgaacgt cctccaacgg
    15241 cgataccagc tgatgattct acaaatgcac caacaggaga agtaccaaat aaggaacccg
    15301 caagcatagc tgttgaatca gaagctaaag ctttccctac acgaggcatc ttgttgtctt
    15361 tcatgaaacc tgcttggttt gctaatccaa ctaaagttcc tgctgtatca aagaaagtca
    15421 caagcaagaa tgtcagcact acgacccaca tttgaagaga attgatgtcc cctacatgtt
    15481 ttaatgcaac taaaaatgtt ggtttcaaac ttggtgctgc ggcaacaatt tcagaaggca
    15541 ttggaatcaa acctgtgacc agacctaaaa tcgttgtagc tgccatcccg atgaagatgc
    15601 cacctggtac acgccgaaca agcaagatag cagttacgac taatccaaaa atcgtcaacc
    15661 aagtagaacc aacgttgagt gatcctaatc ctactaatgt agattcattc gaaacgatga
    15721 tcccaccttc gctcagacca aggaaagcga taaatagacc gataccgcca gaaatcgcat
    15781 atttcaaatc tgctgggatc gcatcaatga tcatttcccg cagcttgaag attgtgatca
    15841 aaataaaaat caaagaagca acgaatacgc cggctaatgc tgtttcccat ggaatcccca
    15901 ttcctaaaca aacagaataa gcaaaaaaag cattgatccc aagagcaggt gccgttccaa
    15961 ttggataacg ggctaatacc cccattaaaa cacagccgat cgcactggct aatgcagtcg
    16021 cagtaaacac tgctccttca tccattccag aagcacctaa tacagttgga tttacaaata
    16081 agatataagc cattgagata aatgttgtaa atcctgccag tacctcacgt tttaccgtgg
    16141 tgttcaattt ttctatttcg aagtaggaaa gtattttttc cttcatcttc catcccccta
    16201 aatccgaaca ttataaataa agtccgttaa aatttcgatt tattaataaa acaacgtgtt
    16261 ttatcatata ctaaaacaaa acataagtaa acaaaaaatt cggattttaa gcaaaatccg
    16321 aacgttccat ctgattaaat ccctttatct caaagttcat gaacattcac tttggattac
    16381 aagaatttct ttattgtgtt aataaagttt cttcattcta gtctcaaaaa tcaatagtgt
    16441 gatttgattt taccatttcg tttctgttct tctttgatga attcttttgt atcgacagga
    16501 ttcagattat ttagtccgcc aaaacctgta tcgaccattt ttccccagcc ttttgtcttc
    16561 ttttttcctg atttgagatt ctttgctcga agcttctcac tatgttttgc tcgccgatac
    16621 ttcaaataac cttctaatct tcccattaga tccactgttt ttcctccttt ttattcaatc
    16681 agacgactca gcttttccta taacaaaata attgccttca gggtcagaaa aattgaaagt
    16741 caagatgcca ttgttatctg tgatttctcc tggttcttcc atgttctcgt agatagtttc
    16801 aaattcatcc gtaaaaaata ctaaagaagg cggtggtcct aatacctctg gtgaaaactt
    16861 ctgcacaaat acttttggga atacgcctaa ttctaattct tttgagatgc tgagtacaat
    16921 actcttaaat tcttctggca attcgattgt ttcagaaatt tctgcaccaa ttttttctac
    16981 aaagaatcgg caaatcatct ctacgtcatt gacataaagc atcacacgtg ttttttgtat
    17041 catcatttga cccttctttc tatttgattt tctttcctgt ctataaactg attccattat
    17101 atcacgaaag cgcaaacacc ttgtgtttga tactattcaa gaaaaataaa gcaatagttt
    17161 ctaggattgc tttcctccac aaactttttt tacaaaaaaa aaacggcaat gaatgccgct
    17221 cttcttattt cgattctttt ataattctgc ttctgattca tctttggtta gtgtgtgtct
    17281 aacactagtt acgccaaatg tttgatcgat tttttccttc aacgcttcga ttgcctgtgc
    17341 gccacgtgcc gcttgttcag cattgatgga ttcaatgacc agcaccgctg cttgtgtttc
    17401 ttccgtcagc atcgttcttt gggcttcacg ccagttgaga cagcgacaga tcgctccttg
    17461 ttcatcatag tagatgattt cttccggaag tgccggagca tcggaatctg cacctaaagg
    17521 caggaatgat tcgccacctt gggcttttcc taaatgcaga tttcctgcga tcttttcgat
    17581 atcttctccg ccgcaaggta cgccaaaaga caaggaaata ctattataga tatctacaag
    17641 cggattgatt gggaaaaatt cccgttcttg atttgcacgt ttcagtaaag cttcaataga
    17701 agaacgtgca ccttttttag tcttgaatgt ggtaaatgcc tgacgccact gagcaatgac
    17761 ttcgttttgg ctgaatgttt cttctgtaag gtaatttctt gcttcttttg ctgcctcatt
    17821 caataattga tgatacgttt cttcactttc ttttgttacg tggttatcga taccatttac
    17881 ggataagatt tcgatggttg cttcaggaaa tacttcccag aaattggtat ccactattac
    17941 ttttttcatc tgttcacacc tcttgattag aataattgct tgatgaatag atagtctgtt
    18001 tggggatcat ctcccatata aaaaacatgt tcgctgtatt tgataaatcc ttttttacga
    18061 taaaaagcga gcgcaacatc gttgtgttcc caaacaccga gccaaatggc tgttttttgc
    18121 ttttcttttg ctttttcaat agctgtatct agtaaaaatg taccgatacc ttgatgttta
    18181 aatttttttc gtacatagat tcgctcgatt tctaaaaaat gatcagccat ttcctcagat
    18241 tgactcgttc ctgtatttag tttcaggtaa cccgcaggct cttgatcgat actcactagg
    18301 aaaaaatcgg tttctttatc ttttagctct tctgtaagtt tttctagtgc ataagcttgt
    18361 gtcaagtaat cagctaaatg ctccggtgaa ttatcttttc caaatgtatc tgtaaatgtt
    18421 tcgatactaa tttgttgtag taagtctaca tctgaaggcg tcatttttcg aatttcgtat
    18481 ttattcacgg cttcttcctt tcaatagatc cttttattgc cttttttgac aaaattccaa
    18541 tcttcgctaa cattcttgca tactttattt aaaagatatt ccaattgttt tgcttcttcg
    18601 tcagaaagac catttaatgc gactgcattg gagtagttgt tttctcgaat gatgaaagga
    18661 tagatttctg ctcctttttt agtcggatag agtttcttga tttttttatt ttctttatct
    18721 tccaaacgtt cgatcatccc attggattct aatttcttga ttgctcgggc agtcgttgta
    18781 cgatctactt tgatcatttc tgctagtttt tcttggatga ttccaggatt ttcgcaaata
    18841 cgtactaaat aaagatactg tcctcttgtt aaatcgactt ctttgaattc gatattgcta
    18901 atagaatcta gcgctcgggc aatcattccg atgtctcgaa ggatttcagc catttcatcc
    18961 cacctttatt ttgtttgtaa gactag
//
LOCUS       DAZKLD010000047        18111 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.047, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000047 DAZKLD010000000
VERSION     DAZKLD010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18111)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18111)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18111
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.047"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..267)
                     /locus_tag="ACG8KR_001317"
     CDS             complement(<1..267)
                     /locus_tag="ACG8KR_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail adaptor protein"
                     /protein_id="HFQ0200696.1"
                     /translation="MANNRRLEETFNDGWLKILTQTTKRNELGKKIGVEDTEITSLKF
                     RNLSMRDSDITAMDAMGSKLTKKVKTPFHPIAKKFNKDQYFIVID"
     gene            complement(257..568)
                     /locus_tag="ACG8KR_001318"
     CDS             complement(257..568)
                     /locus_tag="ACG8KR_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0200697.1"
                     /translation="MTPEQVTEELLIAVKDNIYVTWNEEDESIKKMIAKNAVYLQSKV
                     STTLSFSPESLEYGLLIERCRYDWNRALDEFEQNFASELLGFIQHYALQEYIAGDVNG
                     E"
     gene            complement(565..1440)
                     /locus_tag="ACG8KR_001319"
     CDS             complement(565..1440)
                     /locus_tag="ACG8KR_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0200698.1"
                     /translation="MKYILCQPAINRFKWELEVCLTNLKKLGIKDIVLLFSRHDDQIP
                     IFFEKEYGVEVHVYDDLRDDKEYIPSIKPYLWWKYLEEDHSREDDRYFYIDSDVIFNK
                     RINLRKLPSKDDVWYCSDCCSYLSLDYIRSCENGENILKDMANIVNVTVESLETINTN
                     SGGAQWVINRPKANYWKKVYLDSNRLYRYLRGQKTNIQIWTAEMWAQLWNMMYFNIGP
                     KVHEELDFCFATDPIEKVKEVKILHNAGVTTNDEDLFFKGRYVTSTPFDEDLSFVNKK
                     KCSYAYVKAIKAVVR"
     gene            complement(1450..2610)
                     /locus_tag="ACG8KR_001320"
     CDS             complement(1450..2610)
                     /locus_tag="ACG8KR_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFQ0200699.1"
                     /translation="MTVKNLKGVTAASDQLMKAFKDGNEESFSAAMVSLSKEIQDKIL
                     EEATAKNQDQLVLMNRGQRVLTTQETKFYNEVVKNEGFAGVEELVPATVFERVFEDLE
                     QSHPLLQKITFVNTTGVTEWIVSRGVNPAWWGKLCEAVKKVLDNGFDVINMKQFKLSG
                     YIPVCKAMLDLGPVWLDRYVRTVLVESLRIALEQAIVDGTGKDMPVGMMRDMSKQTSG
                     EYAEKKAEPITALDAVTMGGLMARLSKFNIEGVNDPIYRNVNPSDVVLIVNPTDYWSK
                     VFPAKTVLTANGEYVQVLPVPVSDLQSTAVPEGKAVIGVASDYFMGVGSTLKIEASDE
                     YHFVEDERIYLAKQYANGQPKRNDSFIVLDISALGTTTTTTKPTTTTTTTQA"
     gene            complement(2610..3296)
                     /locus_tag="ACG8KR_001321"
     CDS             complement(2610..3296)
                     /locus_tag="ACG8KR_001321"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0046797 - viral procapsid maturation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head maturation protease, ClpP-related"
                     /protein_id="HFQ0200700.1"
                     /translation="MTTMKTFLAVKNEGAVPQIFIQGFIGSSWFFEGNTDKGIKNILD
                     SLGDQEEIEVVINSNGGDVFQGIAIGNLLKSNKAKVNVVINGLAASAASIIAMAGDTI
                     KIYNNAQLMIHRASTYGEGNVDDFRTIADQLESIDKSVKASYKTRFNGTDEALQELLE
                     KESFMDAETALSYGLVDEIIDAENSSGTEAKKEQSVEEILNDVKEKRAEKIAAFTAAL
                     NKTFGQGDVK"
     gene            complement(3259..4437)
                     /locus_tag="ACG8KR_001322"
     CDS             complement(3259..4437)
                     /locus_tag="ACG8KR_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFQ0200701.1"
                     /translation="MGIFQKAVGYFTKKATVPLEEYFCKLQVDFVYRKFAIETCIDLI
                     ANAMSKAEFKSYEDGKNKKNDLYYRLNVAPNKKNNATEFRKKLIRRLIFYNEVLIVSP
                     SNNSSEIFIADSWDVTEYALKDDVFSQVQINNIVLDREFLESDVIYIKYADQQIRQLV
                     DAYYQAYGKLISSAMNVYKRSNARRYVLKGNLFRPQDNTTQDQINKMMTSQFKAFMEA
                     DNAGAVFQLQNEYTLEDFSGNFQSNSRDIKNLIDDIFEMTAAAFHVPKNLLKGDMSGL
                     SDQVDAFLMFEIIPIAELIQDAFNASLYEVEEYLSGNFVRVDTTMIKITSFKDLVDAI
                     DVGIRNGVFTINEGRERVGNDRSDKAMADEIFITKNNQQVSKGGEANDDNENISSSKE
                     "
     gene            complement(4457..6151)
                     /locus_tag="ACG8KR_001323"
     CDS             complement(4457..6151)
                     /locus_tag="ACG8KR_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase TerL endonuclease subunit"
                     /protein_id="HFQ0200702.1"
                     /translation="MNQFLSYKHIENWFKAIEEGTIKVCKEQLLLKNYLEERVFTRED
                     IYFDKQMVEDSINIPAQYFPFELIPWEKFLQCFIYGVRWKKDKTLVFNRYLSLMGRGN
                     GKTGFASWNNFFLLTAKHGIKNYDIDIYANNESQAKTSFDDVFKVIKDHPDLDKKVFK
                     ATKEVIQNIATNSKLRYNTANARTKDGKRPGANRFDEIHENEDYSMINVATSGGGKIR
                     DYREFYDTTNGHVRGGPLDDIIEESKMILSGELGIDKDGAEFSSLFPFICRLDNDNEV
                     DDPDMWEKACPTINYNADLKRKMFQEYSQMQRNAGLRLTFMTKRMNRPMEDTRFAVAS
                     YDDVLHTKEKEFPEKMDEVIGTVDFADRRDFASVGLLGKYDKDVYFTQHTFIHESALR
                     LQNIKREVIDISIDQGKSQIVHGKNIEADYIVGWFLEMSNKYYIKKIAMDMYRAKILK
                     PALEEAGFTVEIVRSGSVTHGMLKDLVDDLFINQRLFFGDDAIMRWYCMNVYEEHISN
                     GNIRYEKIEPETRKTDGFFSFLHGLNFLDDIYDSAPVTVTNSSVENTGTGFTPLVF"
     gene            complement(6129..6443)
                     /locus_tag="ACG8KR_001324"
     CDS             complement(6129..6443)
                     /locus_tag="ACG8KR_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P27 family phage terminase small subunit"
                     /protein_id="HFQ0200703.1"
                     /translation="MFKNELSQNRYREKLRRSLISQLESQKTNIEPFLDNVDRYISLW
                     ETAISLEEDISENGIRLENGKKNESVALLVSVNKQMGLMLDKLAITPELVGEANESIP
                     EL"
     gene            complement(6546..6827)
                     /locus_tag="ACG8KR_001325"
     CDS             complement(6546..6827)
                     /locus_tag="ACG8KR_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0200704.1"
                     /translation="MIINDNGREYDTEKIEEYSSYTQGLIKRLIYVRYVGIRDLLSDN
                     CCSKYKVNQVREALNKDNNVERIKNVFGYSIEEINYYIDFAEAFIPMVR"
     gene            complement(6832..7176)
                     /locus_tag="ACG8KR_001326"
     CDS             complement(6832..7176)
                     /locus_tag="ACG8KR_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HFQ0200705.1"
                     /translation="MTEEFYRWLLQLTREDRLVKFYQSPKWRRLREKAMKRDHYECQE
                     CRRLGKYHRVENVHHIKEVKDRPDLALDLDNLICLCVEHHNEVHGRYLTALDKQEKKI
                     ESFANFDASERW"
     gene            complement(7202..7369)
                     /locus_tag="ACG8KR_001327"
     CDS             complement(7202..7369)
                     /locus_tag="ACG8KR_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HFQ0200706.1"
                     /translation="MYRPQYLEQKYEVITVHKGNGEKVYEYRRPIKSDTYKRKENNEV
                     IPLYGKRIAKH"
     gene            complement(7565..7729)
                     /locus_tag="ACG8KR_001328"
     CDS             complement(7565..7729)
                     /locus_tag="ACG8KR_001328"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0200707.1"
                     /translation="MPATLNAYDGYDYRKKIVKKLYGATYGNVVSVLSVHHQASVTVA
                     EVEDAAVNGE"
     gene            8225..8500
                     /locus_tag="ACG8KR_001329"
     CDS             8225..8500
                     /locus_tag="ACG8KR_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0200708.1"
                     /translation="MYKPYMISYDLNNPGQKYDKVFEIIKEFGAYIKLQKSFWLVKTN
                     LNPNQMCEKLNTVLDNNDSLFICELQKNYQGRATEENWKFINEHIFS"
     gene            complement(8497..8649)
                     /locus_tag="ACG8KR_001330"
     CDS             complement(8497..8649)
                     /locus_tag="ACG8KR_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0200709.1"
                     /translation="MKLSVEGTPEEIQELLQAIGSSKEQKVSVSNSDIDGICNCLNRE
                     KISNPY"
     gene            complement(8958..9371)
                     /locus_tag="ACG8KR_001331"
     CDS             complement(8958..9371)
                     /locus_tag="ACG8KR_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family phage packaging/lysis
                     transcriptional regulator"
                     /protein_id="HFQ0200710.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIETERDAILAALMALSLISRQILYYSFCVPDSFSNYRI
                     SREVGYSERSIQRMKSEALIEFAEAYKHGKIIAYK"
     gene            complement(9448..9744)
                     /locus_tag="ACG8KR_001332"
     CDS             complement(9448..9744)
                     /locus_tag="ACG8KR_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0200711.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKTRKKSKFKVGDMVRCEA
                     EEFVYPFRGYVKKILSNSAIIRIENTMKCDRSTAKHKHYLAVARLVDMEVIKSK"
     gene            complement(9741..10298)
                     /locus_tag="ACG8KR_001333"
     CDS             complement(9741..10298)
                     /locus_tag="ACG8KR_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HFQ0200712.1"
                     /translation="MNKQEAIKKLESIKAIGNDAIAACYNESINSGITLMKKIDEPQK
                     PVIPQLVAGWLEKSTDPFTKAEKIAYLIKSKDGDSYYFCDWFVRDGIVTQEQGEELLA
                     WATRQSYETLLSLYNGYEVEKEPLWAIKNADGNYLTKCALWGKDGVNYSFECNPSHRL
                     LFTDKATADAAALLVNGTVEEVVER"
     gene            complement(10295..10714)
                     /locus_tag="ACG8KR_001334"
     CDS             complement(10295..10714)
                     /locus_tag="ACG8KR_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YopX family protein"
                     /protein_id="HFQ0200713.1"
                     /translation="MIPKFRAWDKRKNVMRDVAVLHFTKNGKTNFIEYWINPTELKSY
                     HVRNIDLMQSTGMKDKNGVDIFEGDIVLVSVRNGFDYLDNKVCIVKNSIDYSGLVCAT
                     VDEDLEYQIFNTELFEEYTYEVTGNIYENSELLEEQR"
     gene            complement(10711..10956)
                     /locus_tag="ACG8KR_001335"
     CDS             complement(10711..10956)
                     /locus_tag="ACG8KR_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0200714.1"
                     /translation="MIKKLVQFSMDLYDIESGATLSVESDHLIINFGGKRQIILWVVD
                     DVLFPEIVHDFEESKAVEFEIVKKVMELIEKYEEDSE"
     gene            complement(10916..11272)
                     /locus_tag="ACG8KR_001336"
     CDS             complement(10916..11272)
                     /locus_tag="ACG8KR_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1140 family protein"
                     /protein_id="HFQ0200715.1"
                     /translation="MDLITQYSDIILKKIMMKIQKDKKSKERAELVKLEMAETGAGVR
                     SSRHWKAAANIEFYYNEIQKGFDQMRELDRQTNWSKKLHQDRFKFVEKYKEILEEYLR
                     RTANDKKTRSIQHGFI"
     gene            complement(11272..11577)
                     /locus_tag="ACG8KR_001337"
     CDS             complement(11272..11577)
                     /locus_tag="ACG8KR_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0200716.1"
                     /translation="MSVDKKAAMKRIAELTKSESWQEDKEIVAEVQKLGKSMWTEKPK
                     RRTPRKIAIWHDDRILVTGTAEQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEER"
     gene            complement(11574..11735)
                     /locus_tag="ACG8KR_001338"
     CDS             complement(11574..11735)
                     /locus_tag="ACG8KR_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HFQ0200717.1"
                     /translation="MTTEEVIQMRIRSLQREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIHRLEE"
     gene            complement(11732..12034)
                     /locus_tag="ACG8KR_001339"
     CDS             complement(11732..12034)
                     /locus_tag="ACG8KR_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0200718.1"
                     /translation="MDKKAAMQRIIELTYSEDWQNDKEAASEVMRLGRAMWADKSNKP
                     RPRKIAIWHGDKLLVIGTAEQLASLTGLHEKIVRKRARCGYTDAKKRTFRYVEESS"
     gene            complement(12027..12191)
                     /locus_tag="ACG8KR_001340"
     CDS             complement(12027..12191)
                     /locus_tag="ACG8KR_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0200719.1"
                     /translation="MDLKTFTAQIELMHQEALRQSVSYEDKWLNTFHGGRESALDQVL
                     KLLKGECRDG"
     gene            complement(12196..12465)
                     /locus_tag="ACG8KR_001341"
     CDS             complement(12196..12465)
                     /locus_tag="ACG8KR_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0200720.1"
                     /translation="MKIDYLELINEIANYKKGEELDVLRDVYDQLEEAGIEGIKNDRS
                     SWSKLRYYFALYIDTTQLRNLAYTKLLFVDCVKGLQKHLNELEQV"
     gene            complement(12477..13226)
                     /locus_tag="ACG8KR_001342"
     CDS             complement(12477..13226)
                     /locus_tag="ACG8KR_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0200721.1"
                     /translation="MTEHRSYYAIIPANVRYDKRLKPNTKLLYGEITALCNERGFCWA
                     GNEYFADLYGVNKETISRWVSDLIKFGYLNREIIYKEGTNQIINRYLRINQYPIDEKR
                     NTPIDEKVKDNNTSINNTFNNTKEYIRELPPSKKSKAKPVRHKYGEYKNVLLSDEQME
                     KLKTEFPNDYQERIERLSEYCESSGKTYKNYLATIRSWARKEKSEPKNASSGYKRTGR
                     REKLPEWAIDQEAYLKKKALERANRQSKAPF"
     gene            complement(13229..13915)
                     /locus_tag="ACG8KR_001343"
     CDS             complement(13229..13915)
                     /locus_tag="ACG8KR_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative HNHc nuclease"
                     /protein_id="HFQ0200722.1"
                     /translation="MFKPLIDSYSAVLKKFKGKDISATINEEVNIDRLKTMYDGYDGD
                     RVIEIRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTGRKISLK
                     DESNTTVSDANVLANIILDFIFEHHIPFKEGYEILPANQEYYFYKCITKRVCCICGKT
                     GADIDHFDKALGRRKRKEVDHAEYTFAALCRIHHTEKHKIGVINFKNKYQIKGIKLNQ
                     KTIKKLNIGG"
     gene            complement(13921..14592)
                     /locus_tag="ACG8KR_001344"
     CDS             complement(13921..14592)
                     /locus_tag="ACG8KR_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HFQ0200723.1"
                     /translation="MSEIKGTTNFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTETELFPVL
                     DYRNQPVVKPNAMQINNSLKRCFVKALALHGLGLYVFQGEDIPTPPRIDTKKLNMLET
                     ILEAFNEQMGKDMTKTLIEYVNEQTDKLGLLADNVETIEQLSYEQCALMERAIAAKRK
                     ELDKK"
     gene            complement(14585..14926)
                     /locus_tag="ACG8KR_001345"
     CDS             complement(14585..14926)
                     /locus_tag="ACG8KR_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0200724.1"
                     /translation="MEKELSTLDQYLIDPDWGKPKIEETSGRKIRRNLLTNEELAWDQ
                     DDLGNHVTIWDHVYLIHLSKHSNKPEYIYVIEDGLIDALEEYDRDNLIDISYYGPGKK
                     YIAEMEAEFDE"
     gene            complement(15104..15802)
                     /locus_tag="ACG8KR_001346"
     CDS             complement(15104..15802)
                     /locus_tag="ACG8KR_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HFQ0200725.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFEKKHQHVLRDIDALKKDVSNF
                     GQMFVEGNEPDSYGRNRRVFFISRDGFFLLAMGFTGKKAIYFKQKYIEAFNEMEDVIR
                     KNTVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNMKVMGVLGGK
                     ESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLPETAIRMEID
                     QLNRQQRLFGDDNE"
     gene            15889..16359
                     /locus_tag="ACG8KR_001347"
     CDS             15889..16359
                     /locus_tag="ACG8KR_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0200726.1"
                     /translation="MSIISFILSTFLTGIKIKGMRPQLEVELNASYFAADMIYTKVII
                     SNYSTEPAMLVNLELSSANLNRRWSATPFKKLIAKGGSVDDNRIYSEAVPLNIPPKSA
                     ISCYLAFEVGKINFNKLLNHQTKMIFTLNRTQIHKIVDIKNTNFPIEKLVKELN"
     gene            complement(16402..16581)
                     /locus_tag="ACG8KR_001348"
     CDS             complement(16402..16581)
                     /locus_tag="ACG8KR_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0200727.1"
                     /translation="MGKFNRALVFSAPLIIYALELWGSRQALIGTIVYMVWIFMGLDE
                     AEAEYRAKKPTEGAD"
     gene            complement(16568..16906)
                     /locus_tag="ACG8KR_001349"
     CDS             complement(16568..16906)
                     /locus_tag="ACG8KR_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF771 domain-containing protein"
                     /protein_id="HFQ0200728.1"
                     /translation="MQYLEAKIPIPEGYVIISQVDYEELKKADDTGRWMTLPEVLERI
                     NRKYDWFTSRVLKNPRYRNIIDIEKNKNGFVYYPVEGRDTYLFLRSKTLEFLETNFSE
                     ILRRQADGKI"
     gene            complement(16922..17125)
                     /locus_tag="ACG8KR_001350"
     CDS             complement(16922..17125)
                     /locus_tag="ACG8KR_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0200729.1"
                     /translation="MTDIAEITQRDREKIKEYVESSKFLTYTMLAERFGISKSYLSLI
                     LNGKKTSAEANRIIDSIITMYEL"
     gene            complement(17140..17916)
                     /locus_tag="ACG8KR_001351"
     CDS             complement(17140..17916)
                     /locus_tag="ACG8KR_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HFQ0200730.1"
                     /translation="MNTPQIFNFEQNEVRTILVNDEPYFVGKDVASVLGYSNTKDALS
                     RHVDLEDKMGSRITTSGQSREMTIINESGLYSLILKSKLPSAKKFKRWVTSEVLPAIR
                     KHGGYLTPEKVEEALLNPDTIIQLATQLKEERTGRLIAEQKIAEYEPKISYLDSILSS
                     TDSVTISQIAADYGMSPQQMNKLLHKLGIQKKVGNQWLLCKKHMNQGYTKSHTTEIPK
                     ADGGTKIVMNTKWTQKGRLFIYELLKKEGYYPQMDLEEIG"
BASE COUNT         5017 a         3800 c         2698 g         6596 t
ORIGIN      
        1 atcgattacg ataaaatatt gatctttatt aaatttcttg gcgattggat gaaatggagt
       61 ctttactttc ttagttaatt tcgatcccat cgcatccata gctgttatat cactatctct
      121 catggaaaga tttctaaatt ttaaagaagt gatttctgta tcttctacac caatcttttt
      181 tcctagttca tttcttttgg tagtttgcgt caaaatcttt aaccaaccat cgttgaatgt
      241 ttcttcgagt ctacgattat tcgccattca catcacctgc aatatattct tgtagcgcat
      301 aatgttgaat gaaacctaat aactcactag cgaaattttg ttcaaactca tctaaagcac
      361 gattccagtc gtatctacat ctttcgatta gcaatccgta ttctaagctt tcaggagaaa
      421 aagaaagtgt tgtactcact ttactttgaa gataaacagc atttttagct atcatctttt
      481 taattgactc atcttcttcg ttccaggtaa cgtaaatatt atccttcaca gctattagca
      541 attcttcagt cacttgttca ggcgtcatct aaccaccgcc ttaattgctt taacatatgc
      601 gtaagagcat tttttcttgt ttacaaatga taaatcttca tcaaaaggcg tagaagtcac
      661 gtatctccct ttgaaaaata aatcttcatc gtttgttgtt actccagcat tgtgtaagat
      721 ttttacttct ttaacttttt ctattggatc agtagcaaaa caaaagtcta attcctcgtg
      781 aactttagga ccaatattga aatacatcat gttccaaagc tgtgcccaca tctcggctgt
      841 ccagatttgt atatttgttt tttgccctct aaggtagcga tatagccgat tagaatccag
      901 ataaaccttt ttccaataat tcgctttagg acggttaata acccactgtg cgcctcctga
      961 attagtgttt atagtttcca aagattctac tgtaacattt acaatgtttg ccatatcttt
     1021 tagaatattt tctccgtttt cacagcttct aatataatca agacttagat aactacagca
     1081 gtcgctacaa taccaaacat catctttaga aggcaatttg cgcaaattaa ttcttttatt
     1141 gaaaatgaca tccgaatcga tatagaaata tcggtcgtcc tcacgcgaat gatcttcttc
     1201 taaatatttc caccataaat atggtttaat cgaaggaata tactctttgt cgtcccgcag
     1261 atcatcgtac acatgaactt caacaccata ttccttctca aaaaaaatag gaatctgatc
     1321 atcgtgtctg ctgaaaagca atacgatatc tttgattcct agtttcttca gattagttaa
     1381 acaaacttca agctcccatt taaaccgatt gattgccggc tgacaaagaa tatacttcat
     1441 tctgatcacc tacgcttgtg ttgtagttgt tgtggttgtt ggttttgtag ttgtagtagt
     1501 agttcccaaa gcgctaatat ctaatacaat gaaactatcg ttacgtttag gttgaccgtt
     1561 tgcatattgt ttagctagat aaatgcgttc gtcttcaaca aaatggtatt catctgaagc
     1621 ttcaattttt agtgtagatc ctacacccat gaagtaatct gaggctaccc caataactgc
     1681 ttttccttct ggcacagccg ttgactgcaa atctgaaact ggtactggca atacttgtac
     1741 gtattctcca ttagcagtta gtacagtctt agctgggaat actttagacc agtaatcagt
     1801 tggattcaca attaggacca catcagaagg attcacatta cgataaatcg gatcattcac
     1861 accttcgata ttgaattttg atagtcgcgc catcaaaccg cccatagtta cagcatctaa
     1921 agctgtaata ggttctgctt ttttttcagc atattctccg ctagtttgtt tgctcatgtc
     1981 acgcatcatt ccgactggca tatctttacc agtaccatca acaattgctt gttctaatgc
     2041 aattctcaat gattctacta aaacagtacg gacataacga tctaaccata ctggacctaa
     2101 atcaagcatt gccttacata caggaatata acctgatagc ttgaactgct tcatgttaat
     2161 tacatcaaag ccattatcta aaactttttt aacagcttcg caaagtttac cccaccatgc
     2221 tggattgact ccacgtgaca caatccattc tgttacacca gttgtgttaa caaaagtaat
     2281 tttttgcaat agtggatgag attgttctaa atcttcaaat acacgttcaa atacagtagc
     2341 tggcactaat tcttcgaccc ctgcaaaacc ttcgtttttc actacttcgt tatagaattt
     2401 tgtttcttgt gtagttaata cacgctgacc acggttcatt aatactaatt gatcttgatt
     2461 ttttgctgtt gcttcttcta aaattttatc ctgaatttcc ttagataagc ttaccatagc
     2521 tgcgctaaaa gattcttcgt taccatcttt aaaagctttc atcaattggt cgcttgcagc
     2581 tgttacacct tttaaatttt taactgtcat tattttacat ctccttgtcc aaatgtttta
     2641 tttaatgctg ctgtaaatgc agcaattttt tctgctcttt tttctttaac gtcattcaaa
     2701 atttcttcaa cgctttgttc ttttttagct tcagtacctg agctattttc tgcatcgata
     2761 atttcatcga ccaatccata actcaaagct gtttctgcat ccataaacga ttctttttca
     2821 agaagttctt gcaatgcttc atctgtgcca ttgaatcgtg ttttatatga agcctttacc
     2881 gatttatcaa ttgattccag ttggtcagca atcgtacgga agtcatcgac atttccttct
     2941 ccgtatgtgg aagcgcggtg aatcatcaat tgtgcattgt tgtagatttt tatagtatcg
     3001 ccagccattg cgataattga agcagcacta gcggctaagc cgttaatcac aacgttaact
     3061 tttgctttat ttgacttaag taagttccca atagcaatcc cttgaaatac gtctccaccg
     3121 tttgaattaa ttactacttc aatttcttct tgatcaccta gactatccaa aatatttttg
     3181 attcccttgt cagtattccc ttcaaagaac caactagaac caataaatcc ctgaataaaa
     3241 atttgcggta ctgcgccttc attctttact gctagaaatg ttttcattgt cgtcattcgc
     3301 ctcacctcct ttcgatactt gttgattgtt tttagttata aatatttcat ctgccatcgc
     3361 cttatcagag cgatcatttc caacgcgttc tcttccttcg ttgattgtaa atactccatt
     3421 tctaatgcct acatcaatag cgtcaaccaa atctttgaag ctagtaatct tgatcatagt
     3481 tgtatccaca cgtacaaaat tccctgacaa gtattcttct acttcataga gactagcgtt
     3541 aaacgcatcc tgaataagtt cagcaatcgg tatgatttcg aacattaaaa aagcgtccac
     3601 ttgatccgat aacccactca tgtctccctt tagtaggttt ttcggaacgt gaaacgctgc
     3661 tgctgtcatc tcaaagatgt cgtctattaa gttttttata tctcttgaat tgctttggaa
     3721 gtttccgctg aaatcttcta atgtgtactc attttgtaat tgaaataccg cacctgcatt
     3781 atcagcttcc ataaaagcct taaattgtga tgtcatcatt ttattgattt gatcttgtgt
     3841 tgtattgtct tgcggtcgga ataaattccc tttcagtacg tatctacgag cgttagagcg
     3901 cttgtaaaca ttcatggcac tagaaatgag tttcccatac gcttgataat acgcatcgac
     3961 tagttgccta atttgttgat ctgcgtattt tatatagata acatcacttt ctagaaattc
     4021 tctatcaagg actatgttgt taatttgcac ttgagaaaac acatcatctt tcaatgcata
     4081 ttctgtgaca tcccaactat ccgcaataaa tatttcgcta gaattattag acggagaaac
     4141 gatcaatact tcattgtaga atattaatct cctgatcagt ttttttctaa attctgttgc
     4201 attatttttc ttattaggag ctacattcag cctatagtaa agatcattct ttttattttt
     4261 tccatcttca tatgacttga attccgcttt actcatcgca tttgcaatca aatcaataca
     4321 agtttcaatc gcaaattttc gatacacaaa atcaacttgc aatttacaaa agtattcttc
     4381 taaaggaacc gttgcttttt ttgtgaagta tcctaccgcc ttttgaaaaa tccccacttt
     4441 ctcacctcct ttcaagttag aatactagag gagtaaatcc agttcctgta ttttctactg
     4501 agctatttgt gactgttaca ggagcagaat cataaatatc atctaaaaaa ttcaaaccat
     4561 gaaggaatga aaaaaagcca tccgtttttc tagtttcagg ttctattttt tcatagcgta
     4621 tatttccatt agaaatatgc tcttcatata cattcatgca ataccaacgc ataatcgcat
     4681 cgtcaccaaa aaataaacgt tgattaataa aaaggtcatc aaccagatct tttaacatac
     4741 catgtgtaac agatccgctt cgaacaattt ccacagtaaa acctgcttct tctaaagcgg
     4801 gcttcaatat ttttgcacgg tacatatcca tagcgatttt tttaatataa tatttattac
     4861 tcatttcaag aaaccaacct acaatataat cagcttctat attttttcca tgaacgatct
     4921 gtgattttcc ttgatctata gaaatatcta taacctctcg tttgatgttt tgtaatcgaa
     4981 gggctgattc gtggataaaa gtatgttgtg taaaataaac atctttatcg tattttccta
     5041 gcaacccaac gctggcaaaa tctcgtctat cagcaaaatc gactgttcct atcacttcat
     5101 ccattttttc aggaaattct ttttctttcg tatgcagaac atcatcatat gaagcaacag
     5161 caaatcgtgt atcttccata ggtctgttca ttcgtttggt catgaacgta agtcttaaac
     5221 cagcattacg ttgcatttga gagtattctt gaaacatttt ccgttttaaa tctgcattgt
     5281 aattaatagt tggacaagct ttttcccaca tgtcgggatc atcaacttca ttatcgttat
     5341 ccaagcgaca aataaatgga aacaaactag aaaattctgc tccatccttg tcaattccaa
     5401 gttctccaga aagaatcatt tttgattctt ctataatgtc atcaagcgga ccaccacgaa
     5461 catgaccatt agttgtatca taaaattctc tataatctcg aattttacca ccaccagaag
     5521 tagccacatt tatcattgaa taatcttcat tttcgtgaat ttcatcaaag cggtttgcac
     5581 ctggtcgctt cccatctttt gttctagcat ttgccgtgtt ataacgaagt ttgctgtttg
     5641 tagcgatatt ttgaataact tccttcgtag ctttaaatac ttttttatct aaatcaggat
     5701 gatctttaat tactttaaat acatcatcaa aactagtctt tgcttggctt tcattattgg
     5761 catagatatc aatatcataa tttttaatac cgtgtttagc ggttagtaga aagaagttgt
     5821 tccaagaagc aaaaccagtt ttaccattac cacgtcccat taatgaaaga tatctattga
     5881 acactagtgt tttatctttt ttccatcgaa caccataaat aaaacattgt agaaattttt
     5941 cccacggaat taattcgaat ggaaagtatt gtgctggtat attgattgaa tcctctacca
     6001 tctgcttatc gaagtaaata tcttctctag taaagactct ttcttctaga taatttttta
     6061 gcaataattg ctctttgcat accttgatag tgccttcttc tatagctttg aaccaatttt
     6121 caatatgctt ataactcagg aattgattca tttgcttcac ctaccaattc aggagtaatg
     6181 gcaagtttat ccaacatcaa tcccatttgt ttgttgacag aaacaagcaa cgctactgat
     6241 tcattctttt taccattctc cagtctaatg ccgttctcgg atatatcttc ttccagtgat
     6301 atcgccgttt cccataaact gatataacga tcaacattat ctaagaatgg ctcaatattt
     6361 gttttctgac tttccaattg gcttattaaa gagcggcgta atttttctct gtagcgattt
     6421 tgagacaatt cgtttttaaa cattttagcc ctcctttcat gataaagttc gaaaaaatct
     6481 cttttcctga cagccccctc cgtttcatca cccccaaaaa atttgcgatt tattttaagg
     6541 gggggttatc tcaccatcgg aatgaaagct tcagcgaagt caatgtaata attaatctct
     6601 tcaatactat atccaaaaac attttttatt ctttcgacgt tattatcttt attcaacgct
     6661 tctcttactt gattcacttt gtatttacta caacagttat ctgataacag atccctaata
     6721 cctacatagc gaacgtatat caaacgttta attaatccct gagtataaga tgaatactct
     6781 tcaatctttt ctgtatcata ctctctgcca ttatcattga tgatcatgca cttaccacct
     6841 ttcacttgca tcgaagttag caaagctttc tatcttcttc tcttgtttat ctaatgctgt
     6901 aagatatctg ccatgaactt cattatgatg ttcaacacat aaacaaataa gattatctaa
     6961 atctaaagct aagtcaggtc tatccttgac ttcctttata tgatgaacgt tctctactct
     7021 atgatactta cctagtcttc tacactcttg gcattcatag tgatctcgtt tcatcgcttt
     7081 ctctctaagc ctgcgccatt taggagactg atagaactta accaaacgat cttctcttgt
     7141 caactgtaat agccatctat agaattcctc ggtcatgttc cgtctccttt cgcaatctta
     7201 tttaatgctt agctattctt ttgccataca atggaataac ttcattgttt tcctttcgtt
     7261 tatatgtatc gctctttatt ggtcttctat actcatatac tttttcgcca ttacctttat
     7321 gaacagtgat tacttcatac ttctgttcta agtattgtgg tctatacatt gttgtttcct
     7381 cctttgtgca aaaataaaaa gaccactcaa agagtgatct aatatgtaat agtaacttgc
     7441 acgatgcaca aaacgcgtac gaaattgcgc acccctatat ttttaaaccg ccgatgcctc
     7501 ggttgcttaa agtcgctgga gtgggattgc accactcacg agaacttacc aggctctcac
     7561 gaggctactc gccatttacc gctgcgtctt ctacttccgc cacagtgacc gaagcttggt
     7621 ggtgtacaga tagcacactt actacattgc cgtacgtagc accgtatagc ttcttaacga
     7681 tcttttttcg gtagtcgtaa ccgtcatagg catttaatgt cgctggcaag gaatcgaacc
     7741 ttgcatggtt gccgaagcat tgacctagca cacatgctta gcgtctaccc tttccgccac
     7801 agtgacacta taaaattatt cttggctgct actatttttt attttgccca tttttaaatc
     7861 caatcatata gacattaaga cagagcgcaa aaattgaaat tattaatgga atcatttctc
     7921 ttcacccacc tttagttatc gtgtgaataa ttaaaaaaca atagacagca acaaaataac
     7981 attgctttga taatttggta taaaccacta taaatttctt ttcttgcaat tatttttaat
     8041 atatgctaga ttatcaaccg atatagtcac tgcctgtact agcggaaact agtgcaggtt
     8101 ttttgttcta tttactcaaa agttattacg ataaattaat attgtgaaaa taaatactaa
     8161 gcgtataatt ttatttatca gcgagtggtc cgctgaaata taaaacaagg atgtgcaaga
     8221 caacatgtat aaaccctaca tgattagtta tgatcttaat aacccagggc aaaagtatga
     8281 taaagtattt gaaataataa aagaatttgg ggcatatata aagctacaaa aatctttttg
     8341 gttagttaaa actaatttga atccaaatca aatgtgtgaa aaattaaata cagtacttga
     8401 taataacgat tccttattca tctgtgaact gcaaaaaaat tatcaaggta gagctacaga
     8461 ggaaaattgg aaattcatta acgaacatat tttctcttag taaggattag atattttttc
     8521 tctgtttaaa caattgcaaa taccgtcaat atctgaatta gaaacactta ccttttgctc
     8581 cttgctacta ccaatagctt ggagcaattc ttgtatttct tctggagtgc cttctactga
     8641 taatttcatt tttcttccct ccaatacata aattaataga cagcagcagt tgaaagacga
     8701 caagaacatg tttagttttg tgagtgctgc tgtctaatca aaataaacag caaccgatga
     8761 agaatttagg aggagttgaa ttcacatcct tttcttcata attagttgct gtctatctaa
     8821 gaagaagttt aaaacgatga gggagattgc ctcccttcgt ttattttgtc gatcctgttt
     8881 cctaatcttt cgacactacc ataataacac tggtaaatgt ctaaaaaccg ccatcattcc
     8941 gccaaaaaac cgccaaatta tttataagca attatttttc cgtgtttata tgcttctgca
     9001 aactctatta gagcttccga cttcatccgt tgtatacttc tttctgaata acccacttca
     9061 cggctaattc tgtagtttga gaagctatct ggcacacaga agctgtagta gagtatctga
     9121 cgactaatca gactaagagc catcaaagcc gctagaatcg catctctctc cgtttctata
     9181 tccatcatct gaataatcgc gtcttctgcc ttattgccat gcttcggtgc cttcggcata
     9241 tccgttataa tcggcgactt aatatctatc aaagagcgac ctgccatccg ctccaaacgc
     9301 cgaaagttct tcagcacatc tctcgcatta catcttgtct gtttgaaatc tacctctcgt
     9361 aacaattgca tcaagtcaaa ccgctccttt atgtgatata ataaatgtgt tggatttatt
     9421 gaatcagtcg gagcgatccg gcttttttta tttgctcttg attacttcca tgtcaactaa
     9481 tctcgctaca gctaaataat gcttgtgttt tgcagtcgaa cgatcacact tcattgtatt
     9541 ttcaatacga atgattgctg agtttgacag tatcttttta acgtatcctc tgaacggata
     9601 aacgaactct tctgcttcac agcggaccat gtcaccgact ttgaattttg atttcttgcg
     9661 tgttttaggg ttctttgtcg gcatatctag cattaaaccg ccgataccgt gactgctagc
     9721 gtaaaatccg tcttttagtt tcatctttct accacctctt ccactgttcc attcaccaac
     9781 aatgctgcag catccgctgt tgctttatca gtgaaaagca atcgatgaga tggattgcat
     9841 tcaaaactat aatttactcc atcttttccc cataaagcac atttagtaag atagtttcca
     9901 tcggcattct ttattgccca taacggctct ttctcaacct cgtagccgtt gtataggctc
     9961 aatagtgttt catatgattg tcttgttgcc caagcgagta attcttctcc ttgctcttgc
    10021 gtcactatgc catctcgtac aaaccaatca cagaaataat atgaatcacc atctttagat
    10081 ttgattaaat acgctatttt ttcagctttt gtaaaagggt ccgtagattt ctcaagccaa
    10141 ccggccacga gttgtggaat aactggtttc tgcggttcgt ctattttttt cattaacgta
    10201 atacctgaat ttatactctc gttatagcaa gcagctattg catcatttcc tatcgcttta
    10261 atactttcta actttttgat tgcttcctgt ttattcatcg ctgttcctcc aataactcgc
    10321 tattctcgta tatatttccg gtgacttcgt acgtgtattc ttcaaacagc tctgtgttaa
    10381 aaatttgata ctctaagtct tcatcaacag tggcacaaac taatccggaa taatctattg
    10441 aatttttgac aatacagact ttattatcta agtaatcgaa gccatttcgc acgctgacta
    10501 atactatatc cccttcaaat atatccacac cattcttatc tttcattcct gtggattgca
    10561 tgaggtcgat gtttcgcacg tgatatgatt tcaattcggt aggatttatc caatattcaa
    10621 taaagtttgt tttgccgttt ttagtaaaat gcaagacggc tacatctctc attacgttct
    10681 ttcgtttatc ccacgctcta aacttcggta tcattcgctg tcctcctcgt atttttcaat
    10741 caattccatt acttttttca ctatttcaaa ctcaaccgct tttgattctt cgaaatcatg
    10801 aacaatttct ggaaacagta catcatcaac tacccacaaa ataatctggc gctttccacc
    10861 aaaatttatg attagatggt ccgattccac agatagtgtt gctcctgatt cgatatcata
    10921 taaatccatg ctgaattgaa cgagtttttt tatcatttgc tgtcctcctc aaatactctt
    10981 ctaatatttc tttatacttc tctacaaatt tgaaacgatc ttgatgaagt ttcttgctcc
    11041 aatttgtttg ccgatccagc tcacgcatct gatcgaaccc tttttgaatt tcgttgtaat
    11101 aaaattcaat gtttgctgct gctttccaat gcctgctact tcgcactcct gctcctgttt
    11161 cagccatttc taacttaact aattcagctc gttcttttga ttttttatct ttctgaatct
    11221 tcatcatgat tttcttgagg atgatgtcac tgtattgtgt aatgagatcc attatctctc
    11281 ctccacatac ctaaactgtc gtccttttga atcaatccat aagctcctag ctctatccca
    11341 gataatgttt ttgcttaatc cagtaatttc agataactgt tcagcagtac ctgttactaa
    11401 aattcggtca tcatgccaga ttgcaatttt tctcggcgtt ctccgtttgg gcttttcagt
    11461 ccacattgat ttaccgagct tttggacttc tgcaactatt tctttgtctt cttgccaaga
    11521 ttctgacttg gttaattcag caattctttt cattgctgct ttcttatcca cgctcattcc
    11581 tccaatcgat ggatttccct tcttaaattc tctatgtgca aatcgattgc ctttctcgcc
    11641 gtttcattga ccatcactgc ctttgttcgt tccagatcgt caatctcacg ctgaaggctt
    11701 cgaatacgca tttgaatcac ttcttctgtt gtcatgatga ttcctccacg tatctaaacg
    11761 ttctcttctt agcgtctgtg tagccacacc ttgctctttt cctcacgatt ttctcgtgca
    11821 agcctgtgag acttgctaac tgttcagctg tccctatcac tagaagtttg tcaccgtgcc
    11881 aaattgcgat ttttcgtggt cttggcttgt tgctcttgtc tgcccacatc gctcttccaa
    11941 gcctcatcac ttctgaagca gcttctttgt cattttgcca atcttctgaa taagtcaatt
    12001 cgataattcg ctgcattgcc gctttcttat ccatcccgac attctccttt cagtaatttg
    12061 agtacttgat caagtgcgct ctcacgtccg ccatggaacg tgttgagcca cttgtcttcg
    12121 tacgatacac tttgtcttaa agcttcttga tgcattagtt caatctgtgc tgtaaatgtc
    12181 tttaggtcca tctgattaca cctgctcaag ttcattaaga tgtttttgca agcctttaac
    12241 acaatcaaca aatagtaatt tggtatatgc taaatttctt aattgtgttg tatcgatata
    12301 aagtgcgaaa tagtatctga gcttactcca actcgaacga tcattcttaa ttccttcaat
    12361 tccagcttct tcgagttgat catatacgtc tctcaggacg tctaattcct cgcccttttt
    12421 ataattcgct atttcattaa ttagttctag ataatcgatc ttcatttttc cacctcttag
    12481 aatggtgctt ttgattgtct attagctcgt tctagcgctt ttttcttgag ataggcttct
    12541 tggtcgattg cccattcagg aagcttctct cgtcttcctg tgcgcttgta tccactgctt
    12601 gcgttcttag gttcactttt ttctttcctt gcccaacttc gaatagttgc caaatagttt
    12661 ttataagtct taccagatga ttcacaatac tctgacagtc gttcgattcg ctcttggtaa
    12721 tcattaggga attctgtttt gagtttctcc atctgctcat ctgacaaaag aacattttta
    12781 tactctccgt atttatgacg gacgggctta gccttcgatt ttttcgaagg cggtaactct
    12841 cttatatatt cttttgtatt attaaatgta ttattaatag atgtattatt atctttgact
    12901 ttttcgtcaa taggggtatt gcgtttttcg tcaatagggt attgattaat tcgtaggtac
    12961 ctattgatta tttgattggt accctctttg taaatgattt cccgattcaa gtatccaaac
    13021 ttaatcaaat cacttaccca tcgcgatatg gtctctttat tcacaccata taaatctgca
    13081 aagtactcat tgcctgccca acaaaagcct ctttcattac acaaggccgt tatctctccg
    13141 tataacaact tagtatttgg tttaagtctt ttgtcgtacc ttacgttggc tggtataatc
    13201 gcataataac ttcgatgttc tgtcattttt accctccaat atttaacttt ttgattgttt
    13261 tctggtttaa tttgatccct ttgatttgat atttattttt gaaattaatc acacctattt
    13321 tgtgcttctc tgtgtgatgg attctgcaga gtgctgcaaa tgtgtactct gcatgatcaa
    13381 cttctttgcg ctttcgtctt cctagcgctt tgtcaaagtg atcgatgtca gctcctgttt
    13441 tgccacagat gcagcagact ctttttgtga tgcatttgta gaagtaatat tcttgattcg
    13501 caggtaaaat ctcatagcct tctttgaaag gaatatgatg ttcaaagatg aaatctaaga
    13561 tgatatttgc taagacattg gcatcactca cagttgtatt cgattcgtct ttcaggctta
    13621 ttttgcgccc tgtgacacct tcaaaacgaa agtagaagaa ttccttccag aagtccgttg
    13681 gcatgcctgt atcgataaaa atatcgccta tcagcgcata gatgaagttt cgttgctgta
    13741 cggtgaaacg tctaggatca ataaaacgaa tttcaatgac tcgatcacca tcgtagccgt
    13801 catacatcgt ctttagtcga tcaatgttca cttcctcatt gatggttgcg cttatgtctt
    13861 ttcctttgaa ctttttcaga accgctgaat atgaatcgat taatggttta aacactcata
    13921 tcacttctta tctaattctt ttctcttagc tgctattgct cgctccatca aggcacattg
    13981 ctcatagctt aactgttcaa tagtttcaac gttatcagct aagagcccta atttatctgt
    14041 ctgctcatta acatattcga ttaaggtttt ggtcatatct ttacccatct gctcattgaa
    14101 agcttctaga atcgtctcta gcatgtttaa tttctttgta tcgattctag gtggtgttgg
    14161 aatatcttcc ccttgaaata catataatcc cagtccgtgt agagccaatg ctttcacaaa
    14221 gcatcgcttc aatgagttat tgatttgcat tgcatttggt ttaacaactg gttggtttcg
    14281 ataatctaaa acaggaaata attcggtttc cgtgtgtcct ttaaccgtta ctgagacaga
    14341 tacataagtc ccagtttcat ccataagaaa aggtttatat tcctcaacaa gaaagtcttg
    14401 atgagttcca gaaacaaccc tgtagtgttt gtactcatta atagttaccg ttgcctgtgg
    14461 atcattcttt ttcataatct cccacgcgtg agcccaagat aaataatcaa aatttccttt
    14521 tttcttgaga attttattta acttacgact aaaaagtttt tcaaagttcg ttgtcccttt
    14581 gatttcactc atcaaattct gcctccattt cagcaatgta tttcttacct ggtccgtaat
    14641 aagagatatc aatcaagtta tctctgtcgt actcttctag cgcatcaatc aagccatctt
    14701 cgatgacgta aatatattca ggtttattcg aatgcttcga tagatggata agataaacat
    14761 gatcccaaat agttacatgg ttgcctaaat catcttgatc ccaagctagt tcttcattcg
    14821 tcaaaagatt tcgtctgatt tttcgaccac ttgtttcctc aattttcggc ttgccccaat
    14881 caggatcaat caaatattga tctagagtgg aaagttcttt ttccatatgc taaaatctcc
    14941 ttatgatgtg ttttctttgt gactctttgc ttgccggcgg agtcactttt ttatttgttg
    15001 ccatgctttt tgcttgtcaa tatgttgttg gcttaggatg attggtttat agtatttcca
    15061 ccagcaatta gcaattgccg tccctattct tagcgcttca gctctattca ttgtcatcac
    15121 caaaaagtct ctgttgtctg ttcagttgat caatttccat acggatcgca gtttctggca
    15181 accacatttc aataaatgaa acagcatcat cgaatctctt acgaggtaac tcgccatatc
    15241 ttgggattga aaaggtacgt ttaaattcag accaaaattt tgagaatact tttttgctga
    15301 tttcttcata agctcggctt tcttttcccc ctagaactcc cataactttc atatttcctt
    15361 tttgcttaat ttcaaactct tgttgtccgc taattcgcat agtatcttta agcatggaaa
    15421 catctttttt aacatctttc atttcttcta attgatagat catcatatct tcaattgttt
    15481 gaggaacagt attcttgcga ataacatctt ccatttcgtt gaatgcttca atgtattttt
    15541 gtttgaagta gatagctttc tttcctgtaa accccatagc cagcaagaaa aatccatctc
    15601 tactaatgaa gaaaactcgt cgatttctgc catatgaatc tggttcatta ccttctacaa
    15661 acatctgtcc aaaattggac acatcttttt ttagtgcatc aatatctctt aaaacatgtt
    15721 gatgtttttt ctcgaagctt tctgccactt gtaagctcgt agtcacagct tctttatttt
    15781 tcaaaattac taattcttgc attatttctt ctctcctttt tggtataatt tagttaaaaa
    15841 ctggtggtgt ttattttgaa tttttttcac ttaacaattt ttcaatggat gtcaataata
    15901 agttttatat tgagtacgtt tttaacaggg ataaaaataa aaggaatgcg ccctcaacta
    15961 gaagttgaac ttaatgcttc ttacttcgct gcagatatga tttatacaaa agtaatcatt
    16021 tctaactatt caacagaacc agctatgtta gttaatttag aactttcatc cgctaactta
    16081 aaccgcagat ggtcagctac tccatttaag aaattaattg ctaaaggagg atcagtcgat
    16141 gataatagaa tatactctga agctgtaccg ctaaatattc ctcctaaaag tgctatatct
    16201 tgttaccttg catttgaagt agggaaaatt aattttaata aactccttaa tcaccaaaca
    16261 aaaatgatat ttactctaaa tagaacccaa atccacaaaa ttgttgatat caaaaataca
    16321 aacttcccaa tagagaaatt agtgaaagaa ttaaactgag taatagtaat atacattcta
    16381 gtttctcttt tctattcatt tttagtcagc cccctcggtt ggctttttcg ctctgtactc
    16441 agcttcagct tcatcaagcc ccataaaaat ccaaaccatg taaacaatcg ttcctatcaa
    16501 cgcttgcctg cttccccaaa gttctaaagc gtagatgatt agcggtgcgc tgaacactag
    16561 cgctctatta aattttccca tccgcttgcc tccttaagat ttccgaaaaa tttgtttcta
    16621 aaaattcaag tgttttactt cttaaaaata gatatgtgtc tcttccttca actggataat
    16681 agacaaatcc atttttgttt ttctcgatat cgataatatt tctatatctt gggttcttta
    16741 aaactctaga agtaaaccaa tcatattttc tgttaatccg ttctagcact tctggcaacg
    16801 tcatccatct accagtatca tcagcttttt ttaactcctc ataatccact tgggagataa
    16861 ttacatagcc ttctggaatt gggatttttg cttctagata ttgcattagc tgttcctccc
    16921 tttacaattc gtacatagtg ataatcgaat ctataattct gtttgcttct gcagaagtct
    16981 ttttaccgtt taaaattaaa gataagtagc ttttgctaat tccaaatctt tcagcaagca
    17041 tggtgtaagt taagaacttt gaactttcga catattcttt gattttttct ctatctcgtt
    17101 gagtgatttc tgcaatgtca gtcatactaa aactcctttc taaccaattt cctctaaatc
    17161 catttgaggg taatatcctt ctttttttag taattcgtag ataaatagac gccctttctg
    17221 tgtccatttg gtattcatta caattttagt gccaccatcg gctttcggga tctcagttgt
    17281 atgagatttt gtgtatcctt ggttcatgtg ttttttgcac aataaccatt ggttaccgac
    17341 ttttttctga atacctagtt tatgaagtaa tttattcatc tgttgtggag acatcccata
    17401 atctgctgca atctgactaa ttgttactga atctgtagaa gataatatgc tatctaaata
    17461 ggagatttta ggttcgtact ctgcaatctt ttgttctgcg attaatcttc cagttctttc
    17521 ttcttttagt tgagttgcta attgaatgat tgtatctgga ttaagcaaag cttcttctac
    17581 tttttctgga gttagataac ctccatgttt tctaattgct ggcaacactt cacttgttac
    17641 ccaacgttta aattttttgg cagagggaag ttttgatttt aagattaaac tgtacaaacc
    17701 agattcgttg atgattgtca tctctcttga ttgacctgag gtcgtgattc gcgaccccat
    17761 cttatcttcc aaatctacat gccgcgacaa agcatcttta gtgtttgaat aacccaaaac
    17821 actcgcaacg tctttgccta caaaatatgg ttcatcattt actaaaattg tccgaacttc
    17881 gttttgttcg aaattaaaaa tttgcggtgt gttcatattt ctcattcctt tcttttatat
    17941 tcgtaaacaa atttaacaac ttttttctaa attcaattga cagtatctga gtaatactct
    18001 atactaagag cataattaaa taagacataa aaacattgat tttaaagctt tcttggcggt
    18061 ctgcatttac atatcaatag tgttttttgt tgtcttttta gttgttaaat c
//
LOCUS       DAZKLD010000048        17926 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.048, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000048 DAZKLD010000000
VERSION     DAZKLD010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17926)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17926)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17926
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.048"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(14..1123)
                     /locus_tag="ACG8KR_001352"
     CDS             complement(14..1123)
                     /locus_tag="ACG8KR_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WavE lipopolysaccharide synthesis family
                     protein"
                     /protein_id="HFQ0200731.1"
                     /translation="MNKKEILYNILKKYENLPYVFRNMFGHNISKLVSWKLTADCATV
                     EENSEVYAAFQLRSKDMSDVPVMGLVNCCKDVAIVMQGPLCLKDDFSYNTLAYYKKCY
                     PNALIILSTWKDEDIKALKRIEDLGVIVICSEKPEKPGHLNINYQVGNTLPGIVRAKQ
                     LGAKYVCKTRTDQRIYHPNAMAFFCSLLEQYPVNNEDDWKLIQNQRLLLLSMPYGDMF
                     FPYCLSDFLYFGDVDDMINLFSIPSDVREKGAVSRGVSRREISENNLAPEVQLLRSYI
                     SRMGGNEECSIRAYWEFVKNHVITINKNQIDLYWHKYVGRYSNNTIYGTYYIDDSQDA
                     LHCYNFDFINWLNLYTGKYEYKAEYEKYMDFVWEE"
     gene            complement(1148..2293)
                     /locus_tag="ACG8KR_001353"
     CDS             complement(1148..2293)
                     /locus_tag="ACG8KR_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0200732.1"
                     /translation="MGETMNVRLVTVILAIVCLLIASLPAFRKNRYLNFCTLLICVVI
                     IAAYIYSDKTIWVKSRESMSLYYVLLAYPLFCTLINQAWNLDSMLNTICGVTFLSYIL
                     RTSISLIQKFSGVLLYENIYRECAPENWYRGGFLRINPPCFTIIIIPIALYMIERQVV
                     ARRKVKYYLLIASALAYSAVIHGSRSVLVYQIIVLGCYILFKKGSSKRKIAMWVLAGL
                     LVVIFINSTMFSTFVETFTNSTGEYAGSAESRFASVWFFIPKFLQSPLFGTGILTGEE
                     FTFILPGGVRGHLSDIGFFYTITQYGVGILMFDILFIIKGFKTALLASKVGMTGYQYL
                     AFGITLNVLLTGINIDWFYPIFTPAIPVCLAVIEYIYQECRSVANIE"
     gene            complement(2414..3520)
                     /locus_tag="ACG8KR_001354"
     CDS             complement(2414..3520)
                     /locus_tag="ACG8KR_001354"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFQ0200733.1"
                     /translation="MKVLMCESQMPENWYSFDLSKELNQYVDLTLLCKRDVPPLDPKI
                     KSKHILYHKSTNKVFSALVFLKSLFLIWKEIRFGDYDIVHLQLMKSLKYEGGLYVLCK
                     RHYKKLIMTVHNVLPHEVREKDRQEHFNIYSNCDGFIVHNRVTEQHLYAEFPELNAKS
                     YVCPHAIYGDVKHISRAYDKDKFEFILFGQIRPYKGIDVLLNAITELPMDVRKKVHFT
                     IAGQQHPKQDSTDYKKMIEDLKVSDCVLYKPERISDEEMDSMFRNADAAIFPYKEIYG
                     SGALLMAYTYCLPTIVSNVPAFMEETEEGKIGLLFENLNSKSLADTIVKYVNLSPAEK
                     SKIEDDIYELVNRKYTWAASAKLTYEAYKQILNE"
     gene            complement(3531..4304)
                     /locus_tag="ACG8KR_001355"
     CDS             complement(3531..4304)
                     /locus_tag="ACG8KR_001355"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HFQ0200734.1"
                     /translation="MLYFVIPLMSAKAANNWEMVSTLFNRTLWSCYNQTDPDFKIIVA
                     CHEIPKLTHEYDDRVEFIQVSEKDAPIPTNQDEKMIDKGYKTHALAMRLRELGGGFAM
                     MVDADDLVSSHLAEYVNSHPQENGFYVKTGYVYFVGDDYMKVLPKFSSGSACIVKYAV
                     EDLPEAYPEVMTANCDDNPWIIRKRHGGVVPACEEQGRPLKPLPFKAAVYVLGTGDNH
                     SLYGKTTKYQTKMREILEMFIAKRKIKGKLKAEFSVDWL"
     gene            complement(4318..4773)
                     /locus_tag="ACG8KR_001356"
     CDS             complement(4318..4773)
                     /locus_tag="ACG8KR_001356"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HFQ0200735.1"
                     /translation="MNAYKLYKVERWFYLHKMEFFAKIVKALIYVLHNSSISYQTEIG
                     DNCKFLYGGIGCVIGKDTKIGNHVIIGTNVLTGGRSNKPGMPTIGNNVYIATGAKILG
                     NITIGDNVIVGANAVVINDVEPNCSVGGVPARVLKKDIDVNDYCSLNRY"
     gene            complement(5195..6301)
                     /locus_tag="ACG8KR_001357"
     CDS             complement(5195..6301)
                     /locus_tag="ACG8KR_001357"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFQ0200736.1"
                     /translation="MKILIVVTMTNNFGKKGSYNSQEIGLARGLAELGHEVIVYKSVK
                     SDEEVGTEQLSNTAMIHYIKCRYVGSNGFLSTKNFDKNADVLIQFADLQLSVPHVYSW
                     TVKNGIKYIPYNGITRSQSGNKYIKTIIEILAHRNYKIYKKNGCVAKNDRVSEKLVQL
                     GVNKVKLAPVCVDFEQLHKNYEAIDFTTLREKWNIPRNVRVILYVGRFEKDKRPLDMI
                     SLFNKLDEHNYLLMVGTGALLEQTKQQINLLGLQNRVKIIERIPNKDIWELYCLSDVF
                     VNLNYTEIWGMVLLEAMYYRLGIIAVNAPGPQNIILDNRYGMLAKSDDEILTFLKDGR
                     FTNDIIDAAYQRVTTEFRWSKSGEIMLSMLEGDK"
     gene            complement(6319..7080)
                     /locus_tag="ACG8KR_001358"
     CDS             complement(6319..7080)
                     /locus_tag="ACG8KR_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HFQ0200737.1"
                     /translation="MEKNMYKRKRDFTVCGIVGVCATTKNEFVREVFSMPTSPVSATV
                     DLVGVPAIISAKENPKIAEMYSQATMAAIDGMPIVKIGRRKGFTCERCAAPDIMGMVF
                     EEGVRQGKTHYFYGGKDNEVLNKLRANLERDYPGIKIVGMYSPPFRPLNEEEDSKICE
                     EINNLNPDFLWVGIGAPKQEMWMWEHQKKIHNTVMFGVGAGFNFFAGTLEKAPEWMEK
                     ASLEWLFRLVKEPRRLWRRYILGGIKYIYYTIESK"
     gene            complement(7156..8154)
                     /locus_tag="ACG8KR_001359"
     CDS             complement(7156..8154)
                     /locus_tag="ACG8KR_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFQ0200738.1"
                     /translation="MKKTIMVTGGCGMIGSNLVKRLVKEGCWDVYVADNLWRGKLEYL
                     NDEDGHPVIDLDTHFFNADLTDYEQAKKVIGTTEYVVHLADVVAGIDYVFKNQGELFR
                     INNLINSNVFDCCRKAGKEKIKGVIYVGTVCSFPLTRQNTLNPEPLREEELFPALPES
                     AYGWSKLMGQLEMGYLEKETGIPCCILMLHNVYGTPTDFGERSQVIPALIRKAINNPE
                     EPFNVWGSGEQGRAFIHVNDIVNALVLALDKGWGHGHIQIGPSHCTSIKEIAYKIIEI
                     SGKDIKPFFDTSKPEGDKARCADYSKAKEILDWEPTVSLEEGLRESYNWIEKQIKG"
     gene            complement(8173..8826)
                     /locus_tag="ACG8KR_001360"
     CDS             complement(8173..8826)
                     /locus_tag="ACG8KR_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFQ0200739.1"
                     /translation="MLAEKLNKHIDLLIKRYPTLETIKQDIIDGYLIMEECYLNGGKL
                     LVAGNGGSAADSEHIAGELMKRFKTPRPVSKEFADKLVAIDPVRGPELAKNLECGLTA
                     IPLVAHEALSTAYINDVDGLGVFAQQLFGFGRSGDVFLGISTSGNSKNIMSATVVARA
                     LGIKVIGLTGAKGGELATVADVCVKAPEVETYMIQEYHLPIYHCWCLMLEDKFFGNE"
     gene            complement(8836..9846)
                     /locus_tag="ACG8KR_001361"
     CDS             complement(8836..9846)
                     /locus_tag="ACG8KR_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase"
                     /protein_id="HFQ0200740.1"
                     /translation="MIITKTPFRMSFFGGGTDMEDYFKENGGAVLSTTFDKYCYVNVR
                     HLPRFFDYSAELSYSRTERVTSIEDIEHPAIRNAMKMLDMHEIRLTYEADLPARSGLG
                     TSSSFAVGMLNAFYALEGKYADKKKLADEAIYLERVLCNEAGGWQDQIAASYGGFNRI
                     NFNVDGYEVLPVIISPERKKQLNNNLMMFFTGFTRFSSDVQKANNVSGTEDKRVRLKK
                     MYELVDEAEAILTDKNRNLDDFGRQLDVTWRLKKGTGGAISTGSIDELYEKGMAAGAL
                     GGKLLGAGGGGFLVFYVQPEKQDAVRWAMRDLMYIPFEFENGGTRVIHYTPETYIEKQ
                     NG"
     gene            complement(9865..11115)
                     /locus_tag="ACG8KR_001362"
     CDS             complement(9865..11115)
                     /locus_tag="ACG8KR_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HFQ0200741.1"
                     /translation="MKKQYNIAVAGTGYVGLSIATLLAQHHKVTAVDIIPEKVELINN
                     RKSPIQDDYIEKYLAEKELNLTATLDAKAAYQSADFVVIAAPTNYNSRTQHFDTSAVE
                     AVIRLVMEVNPDAIMVIKSTIPVGYTASVRVKTGSKNIIFSPEFLRESKALYDNLYPS
                     RIIVGTDMDDERLVEDAHTFATLLQEGAIKENVDTLFMGFTEAEAVKLFANTYLALRV
                     SYFNELDTYAEMKGLNTQQIINGVCLDPRIGTHYNNPSFGYGGYCLPKDTKQLLANYA
                     DVPENLIEAIVESNRTRKDFIADRVLQMAGYYNYAEANQYDAGKEKPVVIGVYRLTMK
                     SNSDNFRQSSIQGVMKRVKAKGATVVIYEPTLKDGETFFGSKVVNDLEQFKAMCDSVI
                     ANRYDACLDDIKEKVYTRDIFQRD"
     gene            complement(11622..11902)
                     /locus_tag="ACG8KR_001363"
                     /pseudo
     CDS             complement(11622..11902)
                     /locus_tag="ACG8KR_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697748.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
     gene            complement(12461..12571)
                     /locus_tag="ACG8KR_001364"
     CDS             complement(12461..12571)
                     /locus_tag="ACG8KR_001364"
                     /inference="COORDINATES: protein motif:HMM:NF020874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1972 domain-containing protein"
                     /protein_id="HFQ0200742.1"
                     /translation="MNPNGYEWLRAKGSYPVPKYCNYSETLMEWYQLIIT"
     gene            complement(12977..13666)
                     /locus_tag="ACG8KR_001365"
     CDS             complement(12977..13666)
                     /locus_tag="ACG8KR_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HFQ0200743.1"
                     /translation="MESEDLLSRISEVHPNQVWLDKEKVEKRYIYLFFKRMIDIVGSI
                     IGLILASPLMFYVAYRIRKEEPGSPIFFCHTRVGKNGKTFKMYKFRSMCLDAEEKLEE
                     LLDQNEIEGAMFKMKEDPRVTKIGKIIRAKSIDELPQLWNVLKGDMSLIGPRPPLERE
                     VAEYTRYDLQRLYVKPGCSGLWQVSDRNNVSFHKMVELDLEYISHRSLMYDLRLILKT
                     FYVIVKPNGAY"
     gene            complement(13711..14475)
                     /locus_tag="ACG8KR_001366"
     CDS             complement(13711..14475)
                     /locus_tag="ACG8KR_001366"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsB/CapC family capsule biosynthesis tyrosine
                     phosphatase"
                     /protein_id="HFQ0200744.1"
                     /translation="MIDLHCHILPGIDDGAENIDASIAMAEKAIQQGITHILCTPHHN
                     NGKYSNEKSQVISLVASLQAELEKRQLPLTLLEGQEVRITGTLIEDIDRDEILFTDLD
                     DTYLLIEFPTLEVPLYAEQLFFELLQLGKTPVIVHPERNAHFRKDPNHMLPFLDMGCL
                     AQLTAPSYVGVFGKDIQKTAKMMVKHNLVQMAASDAHGVSKRTFYLKECYEQIAKDFG
                     KEKVVQMKQVAKDLINGDEISYPVYEEVKKKKFGLF"
     gene            complement(14503..15201)
                     /locus_tag="ACG8KR_001367"
     CDS             complement(14503..15201)
                     /locus_tag="ACG8KR_001367"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004713 - protein tyrosine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004715 - non-membrane spanning protein
                     tyrosine kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HFQ0200745.1"
                     /translation="MARTQKQKAAQTHAVSLVTLTNPSSPIAEQYRTIRTNIQFASAA
                     GQQIKTIVVTSSGPGEGKSTTAANIAVVFAKSGQRVLLVDADLRKPVIYKTFKLNNAS
                     GLSTVLSSSGSVADEIQRTPVENLSILPSGPKPPNPSELLSSPRMDQILAEARQLFDV
                     VIFDMPPVVAVTDAQIMSSKTDGTLLVVRENMSRKESLTKAKELLDMVQARVLGVVYN
                     GAEHSKDAGYYYGN"
     gene            complement(15214..15993)
                     /locus_tag="ACG8KR_001368"
     CDS             complement(15214..15993)
                     /locus_tag="ACG8KR_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HFQ0200746.1"
                     /translation="MEETISLKEIFETLRKHVITILISMFAGLAISGIVTFFILTPKY
                     SSQAQLIVTLPQSDTANVNDVNTNLQMINTYKDMIVSDLVLNEVKDRLETEDNVKMTA
                     GQIKDAISVNQSQNSQMFSIQATSTNAVTAQQIANTTSQVFQENAKDVMNVDKISIIS
                     GAVANTTPVSPNNKLNLVIGLVLGMMVGVGLAFLFELLDRTVKDEKYVTDTLGFPILG
                     TVPEMSAKELNAGIRKKGPAPTQPKATNDKTAPSRRSRSRV"
     gene            complement(16009..16953)
                     /locus_tag="ACG8KR_001369"
     CDS             complement(16009..16953)
                     /locus_tag="ACG8KR_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFQ0200747.1"
                     /translation="MAKRRRRSRGKKIFLIILAILVVLVLAIVGVGAKLYLDLSHSIE
                     KTYESVERTQGERTKPVDLDKQESFSVLLMGIDTGDLGRVDQGRSDTMMVATVSPEDK
                     QTTIVSIPRDTYVDIVGHDTKDKINHAYAFGGAAMAMDTVQKYVDIPIDHYVSINMKG
                     LKELVDAVGGIEVNNDLTFSQDGYDFTIGKITLNGDQALSYSRMRYEDPNGDYGRQER
                     QRKIVEGVAKKVLSLNGVSNYQSVLNALESNMKTDMSFDEMKKIALDYRDAFGTIKQD
                     QLQGEGFMQDNISYQKVSDQELDRVKKELKDQMNLENK"
     gene            complement(16994..17773)
                     /locus_tag="ACG8KR_001370"
     CDS             complement(16994..17773)
                     /locus_tag="ACG8KR_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0200748.1"
                     /translation="MEKPFVKLLATVAVGTGAIVICLFGYHFNNQRQHHQRINYAESA
                     ITNQKDTVTSLSKEVDKLYSTKEKIFLNPEITEETVSNLSHKLSSIKLSADDYDIKES
                     ELPKEAAAIQEEKKAVLTQLEDAESKLKIQTAVNDLFTKNVSNWQQAVDDVVIKEKVT
                     SADVARVRENMSFFKDSPWKTVVMQYLGFADTQIAQVTQLDQLFDTMLKDGQVTATAT
                     YDQYLTALSLIEQIRNEKIRAAYASKAETVAQQMGYSNTSY"
BASE COUNT         5292 a         3938 c         2765 g         5931 t
ORIGIN      
        1 ctataaaagt taattactct tcccaaacaa agtccatata tttttcatat tctgctttgt
       61 attcgtattt tccagtgtac aaattcaacc aatttataaa atcaaaatta tagcagtgaa
      121 gtgcatcttg gctatcatca atataatacg taccataaat agtattattt gaataacgac
      181 ctacatattt atgccagtac aaatcaattt gatttttgtt aatagtaata acatggtttt
      241 ttacaaactc ccaatatgcc ctaatagagc actcctcatt gccccccatt cgtgaaatat
      301 agcttcgaag aagctgaact tctggagcta aattattttc agatatctcc cttcttgaga
      361 ctcctctaga cacagctccc ttttctctca catcactagg aatcgagaag aggtttatca
      421 tatcatcaac atcaccaaaa tacaggaagt ctgacaaaca atacgggaaa aacatgtctc
      481 catacggcat tgatagtaac agtaatcgct gattttgtat taatttccaa tcatcttcgt
      541 tattaacagg atactgttca agtaaactgc agaaaaacgc cattgcattt ggatggtata
      601 ttctttgatc tgttctagtt ttacaaacat actttgcacc aagctgcttt gctctgacta
      661 ttccaggcaa agtgtttcca acttgataat taatatttaa gtgtccaggc ttttcaggtt
      721 tctcactaca aataactata actccaagat cctctatcct tttcaatgcc ttaatatctt
      781 cgtccttcca tgtcgaaaga ataattaaag catttggata acatttctta tagtaggcca
      841 gagtattata agagaaatca tcctttaaac atagtggccc ctgcattaca attgctacat
      901 ctttgcaaca attaactaaa cccatcactg gaacatcaga catgtccttt gagcgcaatt
      961 gaaacgctgc ataaacttca gaattttctt ctactgttgc acaatcagca gtcaatttcc
     1021 aagatacgag tttacttata ttatgaccaa acatgttcct aaacacatat ggtaaatttt
     1081 cgtacttctt caatatgtta tataaaatct ctttcttatt catgagatgc actcctatta
     1141 atatttccta ttctatgtta gccacacttc tacattcttg atagatgtat tctataaccg
     1201 ctaagcatac aggaatagct ggagtaaata tcggataaaa ccagtctata ttaatccctg
     1261 ttagcaagac atttaatgta attccaaatg ctaaatattg atagccagtc atacccactt
     1321 ttgatgcaag taatgctgtt ttgaaccctt ttataataaa cagaatatca aacataagta
     1381 tacctacacc atattgagta attgtataga aaaatccaat atcagatagg tgtcctctaa
     1441 ctcctcctgg taatataaac gtaaattcct caccagtaag aataccagta ccgaacaaag
     1501 gcgattgcaa aaatttagga ataaaaaacc aaactgaagc aaatcgagat tcagcagatc
     1561 ctgcatattc acctgtactg tttgtgaaag tttcaacaaa tgttgagaac atggtcgaat
     1621 taataaatat gactacaagc aacccagcta aaacccacat tgcaattttt cttttcgaag
     1681 aacctttctt aaaaaggata tagcaaccaa gtacaataat ttgatagaca aggacagatc
     1741 tagaaccatg aataacagca gaatatgcca gtgcgctagc aatcagtaaa taatatttta
     1801 cttttcttct agctacaact tgtcgctcaa tcatatacaa agcaatcggt attattataa
     1861 ttgtaaaaca tggtggatta attcttaaaa agcctccacg ataccaattt tccggggcac
     1921 actcacgata tatgttttca tagagaagta ctcctgaaaa tttctgaata agtgaaatag
     1981 atgttctaag aatgtatgaa agaaatgtta caccacatat cgtatttagc attgaatcca
     2041 aattccacgc ttggttaatt aaagtacaga acaatggata cgccaataat acatagtata
     2101 aactcatact ttccctagat ttaacccata ttgtcttatc tgagtagatg taagctgcta
     2161 ttataacaac acaaatcagt aaagtgcaga aatttaaata tctatttttt ctaaatgctg
     2221 gtaatgaggc tattaacaaa catactattg ctagaatgac tgtcacaagt ctaacgttca
     2281 tcgtttctcc caaccagccg cccaaaggct tcaatatgct tgatgagata atcattagaa
     2341 agaccattag aaactgtatg attttccatt tactaatctg catgttaatt attattcctt
     2401 tctaattgtt ttactactca ttcaaaattt gtttgtatgc ctcgtatgtg agttttgcag
     2461 atgccgccca ggtatacttg cggtttacaa gctcatatat atcatcctct attttgcttt
     2521 tctcagctgg gctaaggttc acatacttta caattgtgtc tgctaggctc ttagaattca
     2581 aattttcgaa aagtaaccct atctttcctt cctcggtttc ttccataaaa gcaggtacat
     2641 tactaacaat agttggtaag caataagtgt atgccataag aagcgctcca cttccataga
     2701 tttctttata tggaaatatt gcggcatctg catttctgaa catactgtcc atttcttcat
     2761 cagaaattct ctctggctta tatagaacac aatctgaaac ctttaaatct tcaatcattt
     2821 ttttatagtc agtactatcc tgtttaggat gttgttgacc tgctatggta aaatgaactt
     2881 tctttcttac atccattgga agttctgtga ttgcattaag cagtacatca attcccttat
     2941 atggacgaat ttgtccaaac aagataaatt caaacttatc tttatcatac gctctagata
     3001 tatgtttaac atctccatag atagcatgtg gacaaacata tgactttgca tttaattctg
     3061 ggaattcagc atataaatgc tgttctgtta ctcgattgtg aacaatgaaa ccatcacaat
     3121 ttgaatatat attaaagtgc tcttggcgat ccttttcacg aacctcatgt ggtaacacat
     3181 tatgaactgt cataattaat tttttatagt gtctcttaca taaaacgtac aatccgcctt
     3241 catatttgag acttttcatc aattgcaaat gaacaatatc ataatcacca aatcttattt
     3301 ccttccaaat taagaacaag cttttcagaa aaacaagtgc cgaaaacact ttatttgtac
     3361 ttttatgata caaaatatgt ttgctcttaa ttttaggatc taaaggagga acatcacgct
     3421 tacacagcaa tgttaaatca acatattgat taagttcttt agaaagatca aacgaatacc
     3481 aattctctgg catttggctt tcacacatca ataccttcat aatactcacc ttataaccaa
     3541 tcaacagaaa attctgcttt cagttttcct ttaattttac gttttgctat aaacatttcc
     3601 agtatctctc gcattttcgt ctgatatttt gtcgttttac cataaagcga gtgattgtcc
     3661 cctgttccta atacatatac tgctgctttg aatggaagag gttttaatgg acgcccttgc
     3721 tcttcacatg ctggaacaac gcccccatgt cgttttctaa tgatccatgg attatcatca
     3781 caatttgctg tcataacttc cggatatgcc tcaggcaggt cttccactgc atattttact
     3841 atacacgcac ttcctgatga gaactttggt agaaccttca tataatcgtc tccaacaaag
     3901 tacacgtatc ccgttttgac ataaaatcca ttttcctggg gatgggaatt cacatattcc
     3961 gccaaatgag aacttacaag atcatctgcg tcaaccatca tagcgaaacc gccgcccaac
     4021 tctcgcaaac gcattgcgag agcatgtgtt ttataaccct tatcaatcat tttttcgtct
     4081 tgatttgtgg gaataggggc atccttttcg ctaacttgaa taaattcaac tctgtcatca
     4141 tattcatgcg tgagtttagg aatttcatgg caagcaacaa tgattttgaa atcaggatca
     4201 gtctgattat aacacgacca taatgttcgg ttaaataagg tactaaccat ctcccaattg
     4261 tttgctgcct tcgcactcat taaaggaata acaaaataca acatacaatc tctcctttta
     4321 gtaacgattc aaactacaat aatcatttac atcaatatct tttttcaata ctctagccgg
     4381 aacgcctcct accgaacaat taggttcaac atcattaatg accacagcat tagcccctac
     4441 tattacattg tcccctattg tgatattacc taaaattttt gcgccagtag cgatatatac
     4501 attgtttcca attgtaggca taccaggctt attggatcta ccaccagtca gtacattcgt
     4561 tccgattata acgtgattac ctatttttgt atcttttcca attacacaac ctatgccacc
     4621 gtataagaac ttacaattat ctccaatttc tgtttgatat gaaattgagc tattatgaag
     4681 tacataaatt agtgccttta caatcttggc aaaaaactcc attttgtgta agtaaaacca
     4741 tcgttctact ttgtataatt tatatgcatt catgttttat ctccccatct ttttagcagg
     4801 taccccccac acttcgtagg gtggaataga cctcaatacc actgtatttg cagcaactac
     4861 agcaccttcc ccaacgcata attctgtgtt agatttacaa agaattttag cacccacaca
     4921 taaaacagcg ttctttttta ctcgaatgat gatacgttca ttactatctg ctcaaccaca
     4981 cgtcacattc tgatagactt taacaccatc ttcaaggatt gtgtcagaat gaatcacagt
     5041 gcctacagaa ttatgaacaa attcaacaga gttccctatc tgtgcacctt taccaataca
     5101 acaaccaaga attttggcta tttttctcgt gcaattatca actatgttta aagcatatcg
     5161 attcctcaat gccattaatt tcgttacttc catatcattt atcaccttcc aacatgctaa
     5221 gcataatttc tcccgacttt gaccatctaa attctgtagt tacacgctga tatgctgcat
     5281 ctatgatatc gttggtaaat cgtccgtctt ttaagaatgt caaaatttca tcgtcagatt
     5341 tagccaacat tccatatcta ttatccaaaa taatattctg tgggccaggt gcattaacag
     5401 caataatccc gaggcgatag tacattgcct ctagcaaaac cataccccaa atttcagtat
     5461 agtttagatt tacaaataca tccgataagc aatataactc ccaaatatct ttgttaggaa
     5521 tacgctcaat tatttttact ctattctgca gtccaagtaa atttatctgc tgcttcgttt
     5581 gctcgagtaa agcaccagtt cctaccatca gcaaataatt atgctcatca agtttattaa
     5641 ataaactaat catatctaac ggtcttttat ccttttcaaa ccgtcctaca tataaaataa
     5701 cacgaacatt gcgaggaata ttccattttt ctctgagagt agtaaaatct attgcttcgt
     5761 aattcttatg taactgttca aaatccacac aaacaggagc aagtttcact ttatttactc
     5821 ctaattgtac taatttttca gaaactcgat catttttggc aacacaccca tttttcttat
     5881 aaattttata atttcgatgc gccaatattt caataattgt ctttatatat ttattgccac
     5941 tttgacttcg cgttatccca ttataaggta tatattttat tccatttttt acagtccatg
     6001 agtatacatg aggtactgat agttgaaggt ccgcaaattg tattagaaca tccgcattct
     6061 tatcaaaatt ctttgttgat aagaatccat ttgatcctac ataacgacac ttgatatagt
     6121 gaatcatcgc cgtattgctt aattgctctg ttccaacttc ctcatcactt tttacacttt
     6181 tatatacaat aacttcgtgg cctaattcag ctaaccctcg agctaatcct atttcctgag
     6241 aattatagga ccccttctta ccaaagttat tcgtcattgt aactactata agtattttca
     6301 tctattttta ttgcccccct acttagattc aatagtatag tagatatact ttatacctcc
     6361 caagatatac cgcctccata gtctacgagg ttcctttacc agtctaaata accactctag
     6421 actagctttt tccatccatt caggagcttt ttccaaagtg cccgcaaaaa agttaaagcc
     6481 ggcgcctaca ccgaacatta ctgtgttatg gatcttcttc tggtgttccc acatccacat
     6541 ttcttgctta ggcgcaccaa taccaaccca cagaaaatct ggatttaagt tgttgatttc
     6601 ctcgcaaatc ttgctatcct cttcttcatt taacggacga aatggtgggg aatacattcc
     6661 aacaattttg attcccgggt aatctctttc taggtttgca cgaagcttat ttaacacttc
     6721 gttatctttt ccaccataga aataatgtgt ttttccctgt ctaacgcctt cttcaaaaac
     6781 cattcccata atatctggtg cagcacatcg ttcacaggta aatcctttgc gccgtccaat
     6841 tttaacaata ggcataccat ctattgcagc cattgtagcc tggctataca tttcagcaat
     6901 tttgggattt tcctttgccg aaataatggc cggcactccc acgagatcaa cagtcgcact
     6961 taccggagat gttggcatgg aaaaaacttc ccttacaaac tcgttctttg tagtagcaca
     7021 aacacctact attccacaca cagtgaaatc acgttttctt ttatacatat tcttttccat
     7081 ctcagtacat ccttactcat atgttcttcc tccgcatcct ctaattacac ggaggaagaa
     7141 cagcttatat acttattaac ccttaatctg cttttcaatc caattgtagg attcacgaag
     7201 accctcttcc aaagaaacag taggctccca atccaaaatc tctttcgcct tagaataatc
     7261 tgcgcaacga gccttatcac cttcaggctt agaagtatca aagaaaggct tgatgtcttt
     7321 gccagaaatt tcaatgatct tatatgcaat ttccttgatg gaagtacagt gagaagggcc
     7381 aatctgaata tgaccatgac cccaaccctt atccagggcg agcaccaatg catttacgat
     7441 atcattgaca tgaatgaaag cacgtccctg ctctccgcta ccccacacat taaaaggctc
     7501 ttcaggatta ttgattgctt tacgaatcag agcaggaata acctgactac gttcgccaaa
     7561 atcagtaggt gtgccataaa cattatggag catcaggata cagcacggaa ttccggtttc
     7621 tttttccaaa taccccatct ccaactggcc catcagtttg ctccagccat aagcagactc
     7681 aggaagagca gggaacaact cctcttcacg gagaggctca ggattcaaag tattctgtct
     7741 ggtcagaggg aaggagcata cggtaccaac atagataaca cccttgattt tctccttgcc
     7801 agccttacgg caacaatcaa aaacattaga attgatcaga ttattgattc ggaacaattc
     7861 gccctggttc ttaaacacat agtcaatgcc agccacaaca tcagccagat gaactacata
     7921 ctcagtcgta ccaataacct ttttagcctg ctcataatca gtcagatcag cattgaagaa
     7981 atgagtatcc aggtcgatga caggatgccc atcttcgtcg ttcaggtact ccaactttcc
     8041 tctccacaga ttatcagcta cataaacatc ccagcagcct tccttcacca gtctcttaac
     8101 aagattggag ccaatcatac cgcaaccacc ggtaaccata attgtctttt tcatttaaca
     8161 ttcctccaaa ttttattcgt ttccaaagaa tttatcttcc agcatcaaac accaacaatg
     8221 atatatcggc aagtgatact cttgaatcat gtatgtttca acttcaggag ccttaacaca
     8281 aacatctgct accgtggcaa gttccccacc ctttgcgccc gtcaagccaa taacttttat
     8341 accgagagcc ctagcaacaa cagttgcgct cataatattc ttgctatttc cggacgtcga
     8401 aataccaagg aatacgtcac cagatctacc aaagccaaac agctgctgtg cgaagacacc
     8461 caaaccatcg acatcattaa tataagcagt agacaatgct tcgtgtgcga ccaaaggaat
     8521 tgccgttaaa ccacactcga gatttttggc taattccgga cctcttacag ggtcaattgc
     8581 aactaactta tcggcaaatt ccttacttac aggacgtggc gtcttaaacc gcttcatcaa
     8641 ctcgccagca atatgttccg agtcagcggc tgatcctccg tttcctgcca caagaagttt
     8701 accaccattc aaatagcatt cctccataat taggtatcca tcgataatgt cctgtttaat
     8761 cgtctcaagt gtaggatatc gtttaatcaa taagtcaata tgtttattaa gtttttccgc
     8821 taacattgtt actccttatc cattctgttt ttcaatataa gtttccggcg tgtagtgaat
     8881 aactctagtt ccaccatttt caaattcaaa cggaatatac atcaaatcac gcatagccca
     8941 acgaactgca tcctgtttct caggttgtac atagaaaacc aagaaacctc caccacctgc
     9001 accgagcaac tttccgccta aagcacctgc agccataccc ttttcatata gttcatcaat
     9061 gcttcctgta ctaattgctc caccagtacc ttttttcaat ctccaagtaa catcaagctg
     9121 tctaccgaaa tcatctaaat ttctgttctt atcagtaaga attgcctcag cttcatctac
     9181 aagctcatac atctttttca gtcttactct cttatcctct gtgccactga cgttgttagc
     9241 cttttgaaca tcagaagaaa atctagtgaa tccggtaaag aacatcatca gattgttgtt
     9301 aagctgcttt ttacgttcag gcgaaataat gacaggcagc acctcataac catccacatt
     9361 gaaattaatt cgattgaacc caccataaga tgcggcaatc tgatcctgcc atccaccggc
     9421 ttcattacac agaactcttt caagataaat ggcttcatca gcaagtttct tcttgtcagc
     9481 atacttaccc tccagtgcat aaaaagcatt caacatacca actgcaaaag aactggatgt
     9541 acccagacca gagcgagcag gtaaatcagc ttcgtatgta agccgaattt catgcatatc
     9601 gagcattttc atcgcattac gaatcgcagg atgctcaata tcttcaatgc tagttactct
     9661 ctcagtacga gaataagata actcagcgct gtaatcaaaa aatctaggca gatgtctcac
     9721 attcacataa cagtacttat caaaagtagt agaaagaact gcaccgccat tctctttgaa
     9781 ataatcctcc atatccgttc caccgccaaa aaaagacata cgaaacggtg tttttgtaat
     9841 aatcataaat actctctcct aatcttagtc tctctggaag atatcacggg tatatacttt
     9901 ttccttaatg tcatcaaggc aagcatcgta gcgatttgct ataacagaat cacacattgc
     9961 cttaaactgt tccaaatcat tcacaacctt actgccaaag aaagtctctc catctttcaa
    10021 ggtcggctca taaatcacaa cagttgcacc tttagctttt acacgcttca taacgccctg
    10081 gatagaactc tggcggaagt tatcagaatt ggatttcatg gtcaaacggt aaacaccaat
    10141 cacaacaggc ttttctttgc cagcatcata ctgatttgct tctgcgtaat tgtaataacc
    10201 agccatctga agtacacgat ccgcaataaa gtccttacga gtacggttag attcaacgat
    10261 tgcctcgatc agattttcag gaacatctgc gtagttggcc agaagctgct tggtatcctt
    10321 cggcaggcaa tagccaccgt aaccaaagga agggttgttg taatgagtac caatacgagg
    10381 atcaaggcaa actccattaa tgatctgctg agtgttcaag cctttcatct ctgcataagt
    10441 gtccagctca ttgaagtagc tgacacgaag agccagatat gtattggcaa acagcttaac
    10501 agcttctgct tcagtaaagc ccatgaacaa tgtgtccaca ttctctttga ttgcgccttc
    10561 ctgtagcagt gtagcaaaag tgtgtgcatc ctcaaccaaa cgctcatcat ccatatcggt
    10621 acccacaata atacgcgaag gatacagatt gtcatacagt gccttacttt cacgcaggaa
    10681 ctcagggctg aaaataatat tcttgctacc agtctttaca cgaacagaag cggtgtatcc
    10741 aacaggaatg gtggacttaa taaccataat ggcatcagga tttacttcca tgaccagcct
    10801 aataacagcc tcaacagcgg atgtgtcaaa atgttgagtg cggctattgt agttagtagg
    10861 agccgcaata acaacaaaat ccgcagactg gtatgctgcc tttgcatcca atgttgcggt
    10921 cagattcagt tctttctcag ccaagtactt ttcaatataa tcatcctgga ttggagattt
    10981 acggttatta atcagttcaa ccttctctgg aatgatatcc accgctgtaa ccttatggtg
    11041 ctgtgccaac agagtggcaa tactcaaacc aacataaccg gtgccggcga ctgcaatgtt
    11101 atactgtttc ttcataatat ttcctctttc caccactgct gtggtcacta ctttcttaac
    11161 aaaatgctgt ttgtgaacag taacctgcta tttatataaa acagcccgaa agctgtgttc
    11221 attcatactt aaaacagcca gctcctacgc cggcaaattc aatttttcat aaatccccta
    11281 cccgtttagg gttatattgc aagtttgagt catttttatt gacctcgttt ggggttataa
    11341 tacattttcg cattgaaatg tacctacatt ttccactcaa ccagtcacca aaaagtttat
    11401 aaattcactt attaccctct attcttactc caaaggtatg tttaacatcg ctttattgac
    11461 ctattttcac cccaaatgga agtttgtttg gtacattatt ggtctattcc caccccaaac
    11521 tgtctatttt gagcgagttt ttgtttttta tcattgcaca ttttcaaggc tcaaattcta
    11581 agcggattta ctggcaggga gacattggta aatacttgaa caatttcacg gctttcatca
    11641 tcacatccta cgataataat tctaattgga taaagattat cattaataat ctactttcac
    11701 gtaaaaattc tgaactaaat aaaatctatt gataatattt ttttcttcca ctttttgtat
    11761 atcctacagg tatagtggat ttaattatcc ccaatgcctc tgaagtaaca gacatcacta
    11821 attcaataac acattcacta ccatagtttg taggaacaaa aatgacaaca aaatctgctt
    11881 ccttatgtga tgtttttcca tctgtccaaa ttttattacc aatgaaatct agtttaaaat
    11941 ccctcttaga aactagttca ctctatattt ttattcagtt tcattagttc tgtttttttg
    12001 tcttcagtca ccatatacat atatatctta attatctatc catttcatcc tacgcattct
    12061 tgcgtaaata caattcactc tgttgagctg atataaatca ttggtggtac aaagataaaa
    12121 tgataactgt ttctatacct ccaaaaattc cagtaaatat atctatagat agtttcaaaa
    12181 aaaattgtct tagtttcata atatttaaga tagtggtaca tactttataa caacgtactt
    12241 ttccaaatat tattttccgt tatgaacatc caatatatta caaagtttaa aagtttagat
    12301 gaactataat cttcatttct gcacagtgga taataaattc ttatagataa aaaatagcct
    12361 cttctaaagt cacaaatacc ttactcccac attttaagta aacactctgc agcattctct
    12421 ttgttataaa atgatagata ttcctcaatt ttatctctta ttatgttatt atcagttgat
    12481 accattccat taatgtttca gaataattac aatattttgg gaccggataa ctcccttttg
    12541 ctcgtaacca ctcatatccg ttaggattca ctaaaagttt accgtttatt tttcgattct
    12601 ttttttgaac cgtcccatga acataccgat tcaacaagct aaattataaa aattggtgtg
    12661 ctgtcattat tttttggaaa atttattaat ggctatcaat atacttttgt atcatactaa
    12721 atagctcgtc agttcctata tcggatatac caatattaaa gcatttagat cattatattt
    12781 aagagtggta tcatcccaaa atttcgaata ttttctttta tataagaata atgatactga
    12841 attgccccat tcacctttct ttttttagat tttctacaag tgattcaaac cctccataat
    12901 tacagttatt acttttgaaa cgataatatg aacatgtgta atctttttta ttaacaatcc
    12961 atcattctat ccctaactaa tatgctccat tgggcttgac aataacataa aacgttttta
    13021 aaatcaacct caagtcgtac attaaacttc gatgactaat atactctaaa tccaactcaa
    13081 ccattttatg gaaactaaca ttatttctat cactaacttg ccacaaacct gaacaaccag
    13141 gttttacata caagcgttga agatcatatc tcgtgtactc agctacttcc ctctccaaag
    13201 gtggccgtgg accaattaga ctcatatcgc cttttaaaac attccatagc tgtggcaatt
    13261 catcaatgct tttggcacgg ataattttac ctattttagt gacacgggga tcttctttca
    13321 tcttgaacat tgcaccttct atttcatttt gatcaagtaa ttcttccaat ttttcttcag
    13381 catctaaaca catcgaacgg aatttataca ttttgaatgt ttttccattt ttaccaacac
    13441 gtgtatggca aaaaaatatc ggacttcctg gctcttcttt tctgattcga taagctacat
    13501 agaacatcaa aggagaagct aaaatcaaac caataatgct tcctacaata tcaatcattc
    13561 ttttaaaaaa taaatagata tatcgttttt caactttttc tttatccaac caaacttgat
    13621 taggatgtac ttcactaata cgagataata aatcttcact ttccatactt tccctctctc
    13681 ctcatttcct ttttatctac gaataaagaa ctaaaataaa ccaaacttct tcttttttac
    13741 ctcttcatag actggataac tgatttcatc cccgttgatc agatcctttg cgacttgttt
    13801 catctgtact actttttcct taccaaagtc cttagcaatc tgctcatagc actcttttaa
    13861 gtaaaaggta cgcttagaga ccccatgggc atcagaagca gccatctgca caagattatg
    13921 tttgaccatc attttggctg ttttctggat gtcttttcca aatacaccga cataacttgg
    13981 tgcggtgagt tgggccaagc atcccatatc taggaatggc agcatatgat tcgggtcttt
    14041 acgaaaatgc gcgttgcgtt ctggatggac gatcactggt gtctttccta gttgtaacaa
    14101 ctcaaaaaag agctgttctg catataacgg cacttccagt gtcggaaatt cgattagaag
    14161 atatgtatca tctaaatctg taaataaaat ttcatctcgg tcgatgtctt cgatcagtgt
    14221 tccggtaata cgtacttctt gtccttccaa taacgtcaaa ggcaattgtc ttttctctaa
    14281 ttctgcttgt aacgaagcga ctagagaaat gacttgtgat ttttcattgc tgtattttcc
    14341 attattatga tggggtgtgc ataaaatatg tgtgatccct tgttgaatcg ctttttcagc
    14401 catggcgata ctggcatcta tgttttctgc tccgtcatcg attcctggca ggatatggca
    14461 atgtagatcg atcatttctc ttgtctcctc tctttttatt tcttaattcc cgtagtaata
    14521 gccagcatct ttgctgtgtt ctgccccatt gtatacaaca ccaaggactc gcgcttgtac
    14581 catatccagc aattcttttg ctttagtcaa tgattctttc cgtgacatat tttcacgtac
    14641 gaccaataag gtgccatccg ttttggaaga catgatctgt gcatctgtta cggcaactac
    14701 tggcggcatg tcgaagatca ctacatcaaa tagttgtctt gcttctgcta aaatctgatc
    14761 cattcttggc gaacttagta gttctgatgg atttggtggt ttaggtccac ttggcagaat
    14821 cgacaaattc tcgactggtg ttcgttggat ctcatctgct acacttcctg atgaactaag
    14881 aacggtactt aatccgcttg cgttgttcaa cttgaacgtt ttatagatga cgggcttacg
    14941 taagtctgca tcgactagca gcacccgttg accagatttg gcaaaaacaa cggcaatgtt
    15001 tgctgcagtg gtcgatttcc cttctccagg tcctgatgaa gtcacaacaa tcgttttaat
    15061 ttgctgacca gctgcggagg caaactggat attcgtccga atcgtacgat attgttcagc
    15121 gattggtgaa gaggggtttg tgagtgtgac caaactgact gcatgagttt gggctgcttt
    15181 ttgtttctgt gttcgtgcca tttttctttc ctcctagact cttgagcggc ttctgcgtga
    15241 tggcgctgtt ttatcgtttg tagcttttgg ttgtgtcgga gctggtcctt ttttacggat
    15301 accagcattc aattctttcg ctgacatttc tggtacagtc cctaagatcg ggaagcccaa
    15361 tgtatccgtc acatattttt catcttttac tgtccggtcg agtaattcaa ataagaaagc
    15421 tagacctacg cctaccatca ttcctaacac taagccaatc actagattca atttattatt
    15481 tggcgaaaca ggtgtcgtat tggcaactgc acctgagatg atcgagattt tatcgacatt
    15541 catcacgtct ttggcgttct cttggaatac ttgtgacgtt gtattagcga tttgttgagc
    15601 cgtcactgca tttgtgcttg ttgcttggat cgagaacatt tgtgaatttt gtgactgatt
    15661 gacggagatg gcatctttga tttgtccagc agtcattttt acattgtctt ctgtttctaa
    15721 acgatctttg acttcattta gtactagatc actgacgatc atatccttat acgtattgat
    15781 catttgcagg ttcgtattga catcattgac atttgcagta tctgattgcg gcagtgtcac
    15841 gatcaattgg gcttgtgagc tgtattttgg cgtcaagatg aaaaacgtca cgatgcctga
    15901 gatggccaag ccagcaaaca ttgagatgag gattgttatc acgtgtttgc gtaatgtttc
    15961 aaaaatttct ttcaagctga ttgtctcttc cattttttct ccactttcct atttattctc
    16021 tagattcatt tgatctttta attctttttt cacacgatca agttcttgat cgctgacttt
    16081 ttggtatgag atattgtctt gcatgaagcc ttctccttgc agctgatctt gcttgatcgt
    16141 accaaatgca tcccgataat ctaaagcaat ctttttcatt tcatcaaagc tcatgtctgt
    16201 tttcatattc gactctagag cattcaaaac ggattgatag ttgcttacac cgttcagact
    16261 caacactttt ttcgctacac cttctacgat ctttctttga cgttcttgtc tgccgtagtc
    16321 accatttgga tcttcataac gcatacgaga ataagacaat gcctgatccc cgttcaatgt
    16381 gattttaccg atcgtaaaat catagccatc ttgtgagaag gtgaggtcat tattgacttc
    16441 gataccacca actgcatcaa cgagttcttt tagacctttc atattgattg aaacatagtg
    16501 atcgattggg atgtctacat atttttgtac ggtatccatc gccatggcag ctccgccgaa
    16561 tgcgtaagcg tggttgatct tgtcttttgt gtcatgtcca acgatgtcga catacgtatc
    16621 acgaggaatg ctgacaatcg ttgtctgttt gtcttcagga ctgactgtgg caaccatcat
    16681 cgtatctgat cttccttgat cgacccgacc taaatctccc gtatcaatcc ccatcaatag
    16741 aacagagaaa gattcttgtt tgtccagatc aactggtttt gttcgttctc cttgggttcg
    16801 ttccactgat tcgtaagttt tttcaataga atgtgagaga tctagatata attttgctcc
    16861 tactcctacg attgctaata ctaatacgac aagaattgct aaaataatta gaaatatctt
    16921 tttccctctt gatcttcttc ttcgttttgc catagtttcc tccgtttgct agccttgctt
    16981 gaattctcgc ttattaatag ctcgtattgc tatagcccat ttgctgagcg actgtttctg
    17041 cttttgacgc gtatgcggct cttatttttt cattccggat ctgttcgatg agactgagtg
    17101 cggtcaaata ttgatcgtaa gtagcagtag ctgttacttg accgtctttc agcatcgtat
    17161 cgaatagctg atctagttgc gtgacttgcg cgatctgagt atcagcgaat cctagatatt
    17221 gcataacaac cgttttccaa ggactatctt taaaaaagct catattttca cgtacacgag
    17281 cgacatctgc acttgtcacc ttttctttga tgacgacatc gtctacagct tgctgccagt
    17341 ttgatacatt tttagtaaat aaatcgttga cagctgtctg gatcttcagt tttgattctg
    17401 catcctctaa ctgcgttaag actgctttct tttcttcttg gatcgcagca gcttctttag
    17461 gtaattctga ttccttgatg tcataatcat ctgccgaaag tttgatagaa gatagtttat
    17521 gtgacaaatt tgaaactgtt tcttctgtta tttcaggatt taggaaaatt ttttcttttg
    17581 tactgtacag cttatctact tcttttgata aagaggtcac ggtgtctttt tgattggtga
    17641 tggcagattc tgcgtaattg atccgttgat gatgttgccg ctgattattg aaatggtatc
    17701 caaacaagca aatcacaata gcgcctgtgc ctaccgccac agtagccaat aacttgacga
    17761 acggcttttc catagattca ctcctaaata ttgatgatag ataaataaag atgtgattca
    17821 ttcccatcag aatgagcgct ttttatttta aaagcataat taaactaata tattcataga
    17881 aatatttata aacaggtttt ccctatataa tcataccaca aaaagt
//
LOCUS       DAZKLD010000049        17871 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.049, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000049 DAZKLD010000000
VERSION     DAZKLD010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17871)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17871)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17871
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.049"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(59..1537)
                     /locus_tag="ACG8KR_001371"
     CDS             complement(59..1537)
                     /locus_tag="ACG8KR_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0200749.1"
                     /translation="MKLDYLIEEEDREKLLLARYLEFEKKNYVVLPKLYETLGLSKFK
                     TRNYIIELNKEFTEFKTKPEIQISEEAEIHVERLNLTDIKNLRRIYFQRSKIARLLTE
                     IVDSKVTIEKFAENHFLSISRAYVKRRELVSFLEQHQIKLKKNILVGEEINIRNVLFG
                     VYFGVFNGFSMPFRESVMNEAKSIVQYFEYLFNLKLSATKRIKFNLLTTISLLRAYGN
                     QPIGKRFFSDEYFKSSEGMKIIKDLSHISFLHIPKEQIIDEISYIMLFLITEEEFELD
                     GAFGLAEAHFHDIHMISQKMSVELIDQLPFSIEISEEERLYIRKELRMGMMHVNRKNW
                     IFDFEMTSFTSKKQLQFFFETYPIFSTAIQKVIQENKRYFLGKDERFMIRLFYDYLFL
                     LVGICPVSYFEAPIHVCIDFSQGSAYTDYIISQVKGFKNYNIVIESRITSRTNIYLSD
                     CLLEKFNKEQIIWKNPPTPDDWEYFGNTIIREKNRHLTEKIS"
     gene            complement(1835..2770)
                     /gene="murB"
                     /locus_tag="ACG8KR_001372"
     CDS             complement(1835..2770)
                     /gene="murB"
                     /locus_tag="ACG8KR_001372"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HFQ0200750.1"
                     /translation="MNKEEIVESLATITILFDEPLMNYTFTKTGGPADVLAFPKKQEE
                     VKQIIDYCRIHDIPWMVLGNASNLIVQDGGIRGVVIMLTEMKQIHVKGTMVIAEAGAS
                     LIDTTYAALAESLTGFEFACGIPGSVGGAVYMNAGAYGGEIKDVFAEVDLLLEDGTLK
                     TLTKDEMVFSYRHSKVQELRAIVLEARFSLQTGDYEAIKARMDELTELRQSKQPLEYP
                     SCGSVFKRPVGHYTGQLIQQAGLQGLKWGGAQVSEKHAGFIVNIDHATATDYIELIAH
                     IQEVIKEKFDVSLETEVRIIGEKQAISETSRSLAN"
     gene            complement(2843..3763)
                     /locus_tag="ACG8KR_001373"
     CDS             complement(2843..3763)
                     /locus_tag="ACG8KR_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFQ0200751.1"
                     /translation="MKIGVIGVGNIAEKAYLPTYAKKQGTLDFYFATRNKETKERIQK
                     EYGFQHLYETIDELLEEKIEACMIHAATKVHYELAKKCLENGIHVYIDKPLSVHLNEV
                     RELQELADKNQIILMIGFNRRFAPMVEELKRIPDKRLIHLQKNRIAAKETTEFVLYDL
                     FLHLIDTAVYLLDEPILRTKYQIRETKEFLEYAILQLETQNQTAILTMDLASGANIEK
                     YQVTSKNGTYEVNDLTQLVIQDADGKKVKEFGDWTNTLVKRGFEPMVEAFFSQIRLGK
                     SETELLKQKEVVRSHELCEEVLKDHYRHQL"
     gene            3876..4628
                     /locus_tag="ACG8KR_001374"
     CDS             3876..4628
                     /locus_tag="ACG8KR_001374"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HFQ0200752.1"
                     /translation="MKFISWNVNGLRAIVNKNFLEVFHELDADFFCLQETKLQAGQID
                     LDLPGYYQYWNYAERKGYSGTAIFAKKPALNATYGMGIDIHDTEGRLITLEYSDFFLV
                     TCYTPNSQSELKRLDYRLEWEEAFYNYLENLKKQKPVIVCGDLNVAHQKIDLKNWKTN
                     QKNAGFTPEERAALSRLLDNGFIDTFRYFYPTQEGVYSWWNYRFNSRKNNAGWRIDYF
                     LTSKDLEPGLADAKIHTDIMGSDHCPVELDLK"
     gene            5369..5902
                     /locus_tag="ACG8KR_001375"
     CDS             5369..5902
                     /locus_tag="ACG8KR_001375"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HFQ0200753.1"
                     /translation="MNFVAMDFETANHQPYSACSLALVMVKNSQIVDEFYTLIQPETP
                     FFWRNVQIHGIHQEDVRNAPKFPEVWQTIQPYFQKNRLVVAHNAAFDTKVLAGCLDYY
                     HLEQPNYLSLCTVKSSRRLFPEFPNHRLNTVCENLNIQLDHHHDALEDSRACAEILLY
                     QEKKFGTDPLKKLVTIK"
     gene            complement(6392..6916)
                     /locus_tag="ACG8KR_001376"
     CDS             complement(6392..6916)
                     /locus_tag="ACG8KR_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase family protein"
                     /protein_id="HFQ0200754.1"
                     /translation="MSEEVGIILREAVPGDAKDILLMMGQVNKETEFLVLDEAELLLP
                     PETLEEELDYIYESNNNLLLLAIYEGTIIGTASVKADSQFRLSHVGEVGISILQEYWG
                     MGLGTLMLEEIISWAKEMGVLFRLELDVQVRNERAVHLYRKMGFQIEAVMPRGARTDL
                     GEFLDVYKMSYLIE"
     gene            complement(6909..7382)
                     /gene="tsaE"
                     /locus_tag="ACG8KR_001377"
     CDS             complement(6909..7382)
                     /gene="tsaE"
                     /locus_tag="ACG8KR_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HFQ0200755.1"
                     /translation="MIELSGLTMTEQFAEAIGHSAMPGDNLILTGDLGAGKTTLTKGI
                     AQGLGITQMIKSPTYTIIREYSQGRIPLYHMDIYRVAASGADLGLDEYFEGDGLSVVE
                     WGNLLEEALPEDYLELILEKSDTDLEKRYVKLISYGEQSETFKQRILEKWAENHE"
     gene            complement(7505..8485)
                     /gene="pta"
                     /locus_tag="ACG8KR_001378"
     CDS             complement(7505..8485)
                     /gene="pta"
                     /locus_tag="ACG8KR_001378"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008959 - phosphate acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HFQ0200756.1"
                     /translation="MELFDSLRFKIVRRGIKIVFPEATDARILGAAARLKAEELVEPI
                     LVGNEEEIRNAAHARGIKTSSFTIISPDNYDKWDEMVDTFVERRNGKASKEQAEKILK
                     DVNYFGTMLTYMGIADGMVSGAIHSTGDTVRPALQIIKTKPGISRTSGAMIMVRGRDQ
                     EKYVFSDCAINVNPTAQELAEIAVDSAKTAELFDIEPKVAMMSFSTKGSAKSPEVDKV
                     VEATKIAKKLAPQYMIDGELQFDASYVPSVAQLKAPDSEVAGQATVFVFPELQSGNIG
                     YKIAQRFGNFEAIGPILQGLNKPVSDLSRGANEEDVYKLSIITAAQTLID"
     gene            complement(8638..9318)
                     /locus_tag="ACG8KR_001379"
     CDS             complement(8638..9318)
                     /locus_tag="ACG8KR_001379"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HFQ0200757.1"
                     /translation="MKEIIHNSWQEVLSSEFSKDYYLHLREFLKKEYASQKIHPDMYH
                     IYEALELTPYEEVKVVILGQDPYHGENQAHGLSFSVQPGVKIPPSLRNIYKELYDDLG
                     IAPVQHGNLVSWAKQGVLLLNTVLTVREGQAYSHRGKGWERLTDTIIEKLNEREKPIV
                     FILWGKPAQEKIKMIDKSRHIIITSPHPSPLSASRGFFGSKPFSKTNDALLALGEEPI
                     DWQLPETV"
     gene            9444..10313
                     /locus_tag="ACG8KR_001380"
     CDS             9444..10313
                     /locus_tag="ACG8KR_001380"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFQ0200758.1"
                     /translation="MIKLIASDMDGTLLDAHMSVSPENAEAIRFANDAGVEFMVATGR
                     NYQEARAALDEVGIDCAMITLNGAQVFDKEGNSLFTVSIPNNQAISVLDILDANGIYY
                     EVATNDGLYSENQAKRIESFASMVATHLPHLTYKMAIAMASAKLELLHITYVDSIRAI
                     LEEKNLEVLKIICFHTEGPAVLGPVGKQISLLGDLAVTSSGQNNIEVNHKNAQKGIAV
                     AHVAHERGISLEEVMTIGDNFNDVSMLQVAGVSFAMGNAEIEVKDYAKYTTDTNLESG
                     VGKAILRAIAEDL"
     gene            10339..10854
                     /locus_tag="ACG8KR_001381"
     CDS             10339..10854
                     /locus_tag="ACG8KR_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LURP-one-related/scramblase family protein"
                     /protein_id="HFQ0200759.1"
                     /translation="MSEFFIQEKQLGRATRTIVKNEKGHSLFLMVGRWGTKGDALSLY
                     AMNGDLVAHIKQISLTFGTRFELYKEFEKVGTMQKIFNWPGDFYYIRRLHWTVQGDIY
                     NHQYQIHHFNQQIMKMDKATLFSGDYYVLDVSNDEDAPVCICIAAVLDYWLYRKDKSR
                     DKPVSYRLNLS"
     gene            complement(10901..11464)
                     /locus_tag="ACG8KR_001382"
     CDS             complement(10901..11464)
                     /locus_tag="ACG8KR_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0200760.1"
                     /translation="MIRFARKEDAPEIAPLILVILKDMELPFLKQTGEQKTLEILEEA
                     ITDPNYRYSYSRGIVEEIDGKVAGVAYGYTDEEESIIDQPLVPILEKYQLDASTRLFI
                     DKETFPNEWYLDSISVSEDFRGQGIGSRLLEALPKLAKKANRSVIGLSVDEKNPKAKK
                     LYERHDFKVVGQRMISGHLYDHMQKNI"
     gene            complement(11519..11698)
                     /locus_tag="ACG8KR_001383"
     CDS             complement(11519..11698)
                     /locus_tag="ACG8KR_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0200761.1"
                     /translation="MEKMKCPNCGKKFAYEEVNNVVEHNDKEMPVVCPYCRTEAARIV
                     THGYFITEKIEDFLK"
     gene            complement(11775..12284)
                     /locus_tag="ACG8KR_001384"
     CDS             complement(11775..12284)
                     /locus_tag="ACG8KR_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0200762.1"
                     /translation="MKKMMFGVAALIVIGITGMTGLLNAAFHMDDISDQEYRISLKKA
                     AAIVKNETKEHNISAIHFAVEKDLENPTNATYEYQFFCEGQVIAVNPSTGKTTVRNDT
                     EKAAEHLFDIEKTEEFKQPQAAMKEAVSYYGKKAKVKEWTLLVKDERPYYKVRLIDGE
                     QDRQVWISA"
     gene            complement(12380..12760)
                     /locus_tag="ACG8KR_001385"
     CDS             complement(12380..12760)
                     /locus_tag="ACG8KR_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFQ0200763.1"
                     /translation="MNLTTIHHIAIIVSDYQKSRDFYVNKLGFEVIRENYRKERDDYK
                     LDLKLGSAELEIFGIANAPQRPNYPEACGLRHLAFKVDDIEETIAELQSLGIESEPIR
                     EDTFTGKKMTFFFDPDGLPLELHE"
     gene            13090..13839
                     /gene="pnuC"
                     /locus_tag="ACG8KR_001386"
     CDS             13090..13839
                     /gene="pnuC"
                     /locus_tag="ACG8KR_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015663 - nicotinamide mononucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015890 - nicotinamide mononucleotide
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HFQ0200764.1"
                     /translation="MKSYFQFLAHQLKGWPQQNYYLFFFSLGCQVMTLVNSPITWLSV
                     ITFIGTTLGVLCILSINAAKSVNGILGILSAICFIIVGFSAKNYLSIGEQIAYMITLD
                     IPVLLSKEWNHNMASKIRKFTGKSWFIAIISTFLVYLVSGFLIGRYTDDPRPWVDAIA
                     FSISLTAGIISFLRYNNQYYWWLASGLAQMVLWYISFTHGSASLAMFVNSSVYLLNDV
                     LAFTVSPWYNQKERERMQKMEEEYYNAQQHA"
     gene            complement(14023..15300)
                     /locus_tag="ACG8KR_001387"
     CDS             complement(14023..15300)
                     /locus_tag="ACG8KR_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0200765.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
     gene            complement(15379..16023)
                     /gene="pcp"
                     /locus_tag="ACG8KR_001388"
     CDS             complement(15379..16023)
                     /gene="pcp"
                     /locus_tag="ACG8KR_001388"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016920 - pyroglutamyl-peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HFQ0200766.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            complement(16222..17166)
                     /locus_tag="ACG8KR_001389"
     CDS             complement(16222..17166)
                     /locus_tag="ACG8KR_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HFQ0200767.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            complement(17168..17854)
                     /locus_tag="ACG8KR_001390"
     CDS             complement(17168..17854)
                     /locus_tag="ACG8KR_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HFQ0200768.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
BASE COUNT         5194 a         3658 c         3093 g         5926 t
ORIGIN      
        1 ttgaaaaaaa cataaatcag ctaataaata tgtgagacaa agctgccatc cataaatact
       61 agctgatttt ttcagttaaa tgtctatttt tttctctgat gatggtattt ccaaaatatt
      121 cccagtcatc tggagtcggt ggatttttcc aaattatctg ttctttatta aatttttcaa
      181 gtaaacaatc agataaataa atattcgtac gagaagtgat tcgactctca atcacaatat
      241 tgtaattttt aaatcctttc acttgagaaa taatgtaatc ggtataagca gatccttgcg
      301 aaaaatcaat acagacatga atcggtgctt caaaatatga taccggacaa ataccgacca
      361 ataaaaagag ataatcataa aataagcgaa tcatgaaacg ttcatctttt cccaagaaat
      421 agcgtttatt ctcttgtatg accttttgga ttgctgtact gaatattgga tacgtctcga
      481 agaaaaactg tagctgcttt ttagacgtaa agctagtcat ctcaaagtcg aatatccaat
      541 tttttcgatt gacatgcatc atacccatac gaagttcttt tcgtatatat agtctttctt
      601 cttcagagat ttcaatcgaa aatggtaatt gatcaatcaa ttccactgac attttttgac
      661 taatcatgtg gatatcatga aaatgagctt ccgcaagtcc aaatgcgcca tctaattcaa
      721 attcttcttc cgtgatcaaa aacaacatga tataagaaat ctcatcaatg atctgctctt
      781 tagggatatg aagaaaagaa atatgtgata aatccttgat gatcttcatt ccttctgagc
      841 tcttaaagta ttcatctgaa aagaaacgtt ttccgattgg ttgattccca taggccctca
      901 acaaactaat agtggttagc aaattgaatt tgattcgttt tgttgcagac agttttaaat
      961 tgaacagata ttcaaaatat tgcacgatac tctttgcttc attcatgacg ctttctctaa
     1021 atggcataga aaaaccatta aagaccccga aatatacccc aaataatacg ttccgtatgt
     1081 ttatttcttc cccaactaat atgtttttct ttaactttat ttgatgctgt tcaagaaaag
     1141 agactaattc tcttcttttt acataggctc ttgatatgct taaaaaatga ttctctgcaa
     1201 atttttcgat cgttactttt gaatccacga tctccgttaa cagtcgagca atttttgatc
     1261 gttgaaagta gattcttctt aaatttttga tatccgtcaa attcagacgt tcgacatgga
     1321 tctctgcttc ttctgaaatt tggatctctg gctttgtttt gaattcagta aattctttat
     1381 ttaattctat gatataattt cttgttttaa atttagacaa acctaatgtt tcatataact
     1441 taggcagcac tacataattc tttttctcaa attctaaata tctagctagc agcagttttt
     1501 ctctatcttc ctcttctata aggtaatcca acttcatatc ctacacctca aaaaatgcct
     1561 cataccaatt acttgatatg cagatgaaag ttcatctttt cacctatact gattttatga
     1621 tggattatgc ttgttttttc atcagttatt ctcatttttt atcttttttt tgagaatatt
     1681 gcaaaaaatg gaaaaattta tgaggaaata ttctatattt tttgttttat tgtgttaaaa
     1741 tgtacaagat ttgcgccatt tgcgtaaaca accacaaaaa agagcaggac ggccaccgtc
     1801 ctgctctttt acttatttta agttccatta cttattaatt ggctaaactt ctgcttgttt
     1861 ctgatattgc ttgtttttct ccaatgatcc gcacttctgt ttctaaagac acatcaaatt
     1921 tttctttgat tacttcttgg atgtgcgcaa tcaattcaat ataatctgtc gctgtcgcat
     1981 gatcgatatt gacgatgaat ccagcatgtt tttcagagac ttgtgcaccg ccccatttca
     2041 ggccttgcaa tcctgcttgt tggatcagct gacctgtata gtgtcctact gggcgcttaa
     2101 agacacttcc gcatgaaggg tattctaatg gctgttttga ttgacgcaat tcggtcaact
     2161 catccattct tgctttgatt gcttcataat cccctgtttg caaactgaat cgagcttcca
     2221 atacgatagc acgtaattct tggactttac tgtgacgata agaaaatacc atttcgtctt
     2281 ttgtcaaagt tttcaatgtt ccatcttcaa gcaaaagatc cacttcagca aaaacatctt
     2341 tgatctctcc gccatacgca cctgcattca tataaaccgc tccaccgaca cttcccggga
     2401 tgccgcaagc aaattcaaat ccagtcaatg attctgccaa agctgcataa gtcgtatcaa
     2461 tcagtgaggc acctgcttcc gcaatgacca tcgttccttt gacatgaatc tgtttcattt
     2521 cagtcagcat gatgactaca ccgcgaatcc cgccatcttg aacgatcaga ttgcttgcat
     2581 ttcctaatac catccaaggt atatcatgga ttcggcaata atcgatgatt tgctttactt
     2641 cttcttgttt tttcgggaat gcaaggacat cagctggtcc acctgttttc gtaaacgtat
     2701 agttcatcaa tggttcatca aacaatatag taatcgtggc taagctttca actatttctt
     2761 ctttattcac tgaggatttt cctttctttt ctcaaacgtc ctcattattc tatcacgctt
     2821 aatacaatct tgccagtctt gttcaaagtt gatgacggta gtgatcttta agcacttcct
     2881 cacataattc atgacttctt acaacttctt tttgtttaag caattctgtt tccgatttac
     2941 ctaaacggat ctgagaaaag aaggcctcta ccattggctc aaacccgcgt ttgactagcg
     3001 tgtttgtcca gtcgccaaat tctttcactt tttttccatc agcatcttga atgacaagct
     3061 gggtcagatc gtttacttca taagtaccat ttttacttgt tacttgatat ttttctatat
     3121 tagcgccact tgctaaatcc atcgttaaaa tagccgtttg attttgtgtt tctaattgta
     3181 aaatggcata ttccaaaaat tcctttgttt ctcttatctg gtattttgtt ctcaaaatgg
     3241 gttcatctaa taaatacaca gctgtatcaa taagatgtaa aaacaaatca taaaggacga
     3301 actctgtcgt ttcttttgcc gctattcgat ttttttgcag gtggatcaac cgcttatctg
     3361 ggatacgttt caattcttct accataggag caaagcgccg gttgaaaccg atcatcaaaa
     3421 ttatttggtt cttatcagct aattcttgca attcacgaac ttcatttaga tggacactca
     3481 gtggtttgtc aatataaaca tggataccat tttctagaca ttttttggct aattcataat
     3541 gtacttttgt tgctgcgtgg atcatacatg cttcgatttt ttcttctaaa agctcatcga
     3601 tcgtttcata tagatgttgg aacccgtact ctttttgaat tctttctttt gtttctttat
     3661 ttcttgttgc aaaataaaaa tcaagtgttc cttgtttttt cgcataagtt ggtaaatatg
     3721 ctttttctgc gatattccct acaccgatta caccaatctt catctacttt cctcctggat
     3781 catttagtta ctttcatctt acaccatttt ttactaaaat gaggtacaat agtatcgcaa
     3841 aggtaaggaa attaattcga aggagtgtgc ttttattgaa atttatctct tggaacgtaa
     3901 acgggcttcg tgccatcgta aataaaaatt ttttagaagt ttttcatgag ttagacgccg
     3961 attttttctg tctgcaggaa acgaaactcc aagcaggaca aattgattta gatcttccag
     4021 gttattatca atattggaat tacgcggaac gaaaaggcta ttccggcaca gcgatttttg
     4081 ctaaaaaacc agcattgaat gccacttacg gaatgggtat cgatatccat gatacagaag
     4141 gccgtctgat tactttggaa tattccgatt tctttcttgt aacctgttac acgccaaatt
     4201 ctcaaagcga attaaaacgt ttagattacc gattagaatg ggaagaagcc ttttataatt
     4261 atttggaaaa tctaaaaaaa caaaaaccag tcatcgtgtg tggggatcta aatgtcgctc
     4321 atcaaaagat tgatttgaaa aactggaaaa cgaatcaaaa aaatgctgga ttcactccag
     4381 aagaaagagc ggctctttct cgactgttgg ataacgggtt tatcgataca ttccgttatt
     4441 tttatccaac ccaggaaggt gtctactctt ggtggaacta ccgattcaat tcaaggaaaa
     4501 acaatgctgg ttggcggatc gattactttt tgacgagcaa agatttagag ccaggactag
     4561 ctgatgcaaa gatccatacg gacattatgg gcagtgatca ttgtccagta gaattagatt
     4621 tgaaataaag aggttgtgaa aaagtcgttt agtttcgagt attaagaaaa attttgtgaa
     4681 aatggctttc cacattttgc ataaatttgg cataataccg agaaactggc ttttgaacac
     4741 tgtttatcga gagattgtga aaaagctgtc ctgcatcaag taataagaac agccaaacaa
     4801 aaatagttcc tcatattttt cgtttggttg ggcttattac cgcagataca agcttttgaa
     4861 caccgtttat taagaggttg tgaaaaagct gcactgcatc aagtaataag aacagccaaa
     4921 caaaaatagt tcctcatatt tttcgtttgg ttggacttat taccgcagat gcaagctttt
     4981 gaacaccgtt tattgagaag ttgtgaaatc agcgtcctga cctgagtatt agagaaacaa
     5041 atggcaaaat agttcctcat attttgctat tttttgagct aataccgagg gtcagctgat
     5101 tgaacaccgt ttagtggaga agttgtgaaa aagtcgccta ctttcgagtc gtaagaaatt
     5161 tttttgaaaa tggttcctca caactttgca taaatttgac ttaataccga gaagtcatga
     5221 tccccatcta actccaaatc acaaggaatg agttcaaaaa ctagcttttc tcaacaaata
     5281 ttacataaaa aattctatgt tttttgttaa atttatttgc ttttttcaaa agtatgttac
     5341 agtaatttcc caaggaggtt ttacagaaat gaattttgtt gccatggatt ttgaaactgc
     5401 caatcatcag ccttatagtg cttgttctct tgcgctagtc atggtaaaaa atagccagat
     5461 cgttgatgaa ttctatacat tgatacagcc tgagactcct tttttctggc gaaatgtcca
     5521 aattcacgga atccatcaag aagacgtacg caatgcgccg aaattcccag aagtctggca
     5581 aactatccaa ccatattttc aaaaaaatcg tttagtcgtt gcacataatg ctgcttttga
     5641 tacaaaggtc ttagcgggat gcttagatta ttatcatctt gaacagccta attatctttc
     5701 tttatgtaca gtgaaaagta gccgtcgcct gtttcctgaa tttccaaatc atcgattgaa
     5761 cactgtgtgc gaaaatttga acatccaatt agatcatcac catgatgcat tggaagatag
     5821 tcgtgcatgt gcggaaatcc tgctttatca agaaaaaaaa ttcgggacag atccattgaa
     5881 aaaacttgtg actataaagt aaaagaatac ttatgaaaca cacaaagagg tcgtgagcct
     5941 gccgtttcac tccgagtcac aaggagcaat ttcaaaaaat agcttctcat gtttattaaa
     6001 tattgtgact ggtgaccgag gagctggcag gagaacaccg tttattggag aggttgtgaa
     6061 aaagctgttc tgcatcaagt aataagaaca gccaaacaaa aatagcttct catatttttc
     6121 atttggttgg acttattacc gcagatgcaa gcttttggac accgtttacc aagaggttgt
     6181 gaaatcagcg ttttgacctg agtattagag aaacaaacgg caaaatagtt cctcatattt
     6241 tgccgttttt tgagctaata ccgaaggtca gctgattgaa caccgtttat tcaaaaaaaa
     6301 tggactgcga cacgactttt gtcacagtcc cttcgtgtgt ttttctgcct tctcaaaagg
     6361 atgacagttt ctcactcttt tcctatttct tctattcaat caaataactc attttatata
     6421 catctaaaaa ttcacctaga tctgttcttg cccctctagg catgaccgct tcgatttgga
     6481 agcccatttt tcggtaaagg tgtacggctc tttcattacg aacttgaacg tcaagctcca
     6541 atcgaaaaag tacacccatt tccttcgccc aactgatgat ttcttctagc ataagagtgc
     6601 ctagtcccat tccccaatat tcctgcagaa tactgatacc gacttctccc acgtgagaca
     6661 aacgaaattg actgtctgcc ttgacagatg ctgttccaat aatcgtccct tcatagattg
     6721 caagcagcaa taaattatta ttgctttcgt atatataatc caactcttct tctaaggttt
     6781 ctggtggtaa caggagttct gcttcatcca aaactaaaaa ttctgtctcc ttattaactt
     6841 gtcccatcat caaaagaatg tcttttgcat ctcctggcac tgcttcccgc aagatgatcc
     6901 caacttcttc actcatgatt ttcagcccat ttttctagaa ttcgctgttt gaaggtttca
     6961 ctttgctccc cgtatgagat tagtttcaca taccgctttt ctaaatctgt atcacttttt
     7021 tctaaaatca gttccaaata atcctccggc agtgcttctt ctaatagatt tccccattca
     7081 acaacggaaa gcccatcacc ttcaaaatat tcatctaacc ctaaatctgc tccgctagct
     7141 gctacccgat aaatatccat atgatacaac ggaatccgac cttgactata ttctctaata
     7201 atcgtgtatg tcggactttt gatcatttgc gttatcccta atccttgggc aattcctttc
     7261 gtcaacgttg ttttacccgc acctagatca ccagtcaata tcagattgtc acctggcatt
     7321 gcggaatgac caattgcctc tgcgaattgt tctgtcattg ttaatcctga tagttcgatc
     7381 atttttcatc ttctcccgct cttctttctt tttgccagta tatcgcgtaa tgaagttttt
     7441 ttaaagaaaa aatgaactag tcaagaacag cagctgctgt ttttggctag ttcgcgtgta
     7501 tttgttaatc aatcaatgtt tgtgctgctg tgataatcga taatttatat acatcttctt
     7561 catttgctcc acgagacaaa tcagaaaccg gtttgttcaa gccttgtaga attggtccga
     7621 ttgcttcaaa gttaccgaaa cgctgagcaa ttttatagcc gatatttcct gactgcaatt
     7681 ctggaaatac aaagactgtt gcttgaccag ctacttctga atctggcgct ttcagttgtg
     7741 caactgaagg aacataagaa gcatcaaatt gcaattcgcc gtcgatcatg tattgtggcg
     7801 ctagtttttt agcgatttta gttgcttcaa ctactttgtc tacttctggc gatttcgctg
     7861 agccttttgt cgagaagctc atcattgcta cttttggttc gatatcgaac aattctgctg
     7921 tttttgcaga atctacggcg atttcagcca attcttgcgc agttggattc acgttgatcg
     7981 cacaatccga aaagacatat ttttcttgat ctcttccacg taccatgatc atcgctccgc
     8041 ttgtccgact gatgccaggc tttgttttga tgatctgaag tgctgggcga acagtgtcgc
     8101 cagtcgaatg gatcgctcca gagaccattc cgtctgcaat ccccatatac gtaagcatcg
     8161 taccaaagta attcacatct tttaaaatct tttctgcttg ttctttggat gcctttccat
     8221 ttctacgttc aacaaaagta tccaccattt catcccactt atcgtaatta tcagggctga
     8281 tgatcgtaaa gctagatgtt ttgattcctc tggcatgcgc tgcatttctt atttcttctt
     8341 cgttccctac taaaatgggt tccacaagtt cttctgcttt caaccgggca gctgcgccta
     8401 aaatacgagc atcagtagct tccgggaaaa cgattttgat cccacggcga acaattttaa
     8461 atcttaaact atcaaacaat tccacaagat gacctccaga cgtttttata tgtattatca
     8521 tacactattc cagaaaaaaa gaacagttta ttttatctgt atcagtcaaa aatgctggaa
     8581 aaattaagtt tttcacatac aaagcgaact gcactataac aggaaagcgc tgttttatta
     8641 cactgtttca ggcagttgcc aatcaatcgg ttcttctcct aacgcaagga gcgcatcatt
     8701 cgttttcgag aagggtttgg aaccaaaaaa gccacgactt gcagataacg gactgggatg
     8761 tggagatgtt atgatgatat ggcgactctt atcgatcatt ttgatttttt cttgtgccgg
     8821 ttttccccac aagataaaca cgattggttt ttcccgttca tttaattttt caatgatcgt
     8881 gtctgttaat ctttcccaac ctttcccgcg atgagaatac gcttgccctt cccgaacggt
     8941 aagaacggtg ttgagtaaaa ggactccctg tttagcccat gacactagat ttccatgttg
     9001 aacaggtgcg attccaagat catcataaag ctctttatag atatttctta acgatggcgg
     9061 gatctttacg ccaggttgca cagaaaaaga taagccatga gcttgatttt ctccatgata
     9121 aggatcttgt cctaaaatca ccactttaac ttcttcataa ggagtcaatt ccaatgcttc
     9181 atagatatga tacatatctg gatggatctt ttgggacgca tattcttttt ttaaaaattc
     9241 tcttagatgc agataataat cttttgaaaa ttctgaggac aaaacttctt gccaactgtt
     9301 atgaatgatt tctttcaaaa actccacctc ctaataaaaa gaataactca cattttcttc
     9361 aatcacaagg gacaatcttc accctcagca cttgattaat atggtaaact aggaacaaga
     9421 agcagaaagg aacgataatc attatgatca aattaattgc atcagatatg gacggcacat
     9481 tattagatgc ccatatgagt gtttctcctg aaaatgccga ggcgatacgt tttgcaaatg
     9541 atgccggtgt tgagttcatg gtcgctactg gtcgaaacta tcaagaagct cgcgcagctt
     9601 tagatgaggt aggaatcgat tgtgcgatga tcactttgaa cggggcacag gtatttgata
     9661 aagaaggaaa ttcattgttt accgtgtcga ttccaaataa tcaagcaatc tctgttttag
     9721 atattctaga tgctaacggt atttactatg aagtagcaac caacgatggt ctttattctg
     9781 aaaatcaagc gaaacgaatt gaaagttttg cttccatggt ggctacccat ttacctcacc
     9841 ttacgtataa aatggcgatt gccatggcct cagctaagct ggagttattg cacatcacat
     9901 acgtggacag tattcgtgct attttggaag aaaagaatct ggaagtttta aaaattattt
     9961 gttttcatac agaagggcca gctgtattag gtccagttgg aaagcagatt tctcttttag
    10021 gcgatctagc cgttacttcc tctggtcaaa acaatatcga agtgaaccat aaaaacgctc
    10081 aaaaaggaat tgctgttgcc catgtcgctc acgagcgtgg gatttcttta gaagaagtca
    10141 tgacgattgg cgataacttc aatgatgtca gcatgctgca agtagcagga gtcagtttcg
    10201 caatgggaaa tgcagaaatc gaagtaaaag attatgccaa atatacaacg gacacaaacc
    10261 ttgaatctgg cgtaggaaaa gccatattgc gcgcgattgc agaagacttg tagttttatc
    10321 tagaaaagag ggatatagat gtcagaattt ttcattcagg aaaagcagct tggacgtgca
    10381 actcggacaa ttgtcaaaaa cgaaaaagga cattccctgt ttttgatggt tggtcgctgg
    10441 ggaacaaagg gcgatgcatt gtctctttat gccatgaatg gcgatctcgt tgctcacatc
    10501 aagcagataa gcctgacatt cggtacgcgt tttgagctgt ataaagagtt cgaaaaggtg
    10561 ggaaccatgc agaaaatatt caattggccc ggtgactttt attatattcg tagacttcac
    10621 tggacagtcc aaggtgatat ctataatcat caatatcaaa tccatcattt caatcagcaa
    10681 atcatgaaaa tggataaagc aacacttttt tcaggagatt actatgtatt ggatgtctca
    10741 aatgatgaag atgctccagt ttgtatttgt attgctgcgg ttttagatta ctggctctac
    10801 cgtaaagaca aaagtcgcga taagcctgtt tcttaccgac ttaacttaag ttaaaaagat
    10861 gtacctgaaa tgcggaaata cgcatttcag gtacatcttt ttagatattc ttttgcatat
    10921 gatcataaag atgaccactg atcatccgct gaccgacaac tttgaagtca tggcgttcgt
    10981 ataatttttt agcctttgga tttttttcat ccacacttag accgatgacg gagcgattag
    11041 ctttcttcgc caatttaggt aatgcttcta ataatcgcga accgatgcct tgtcctctaa
    11101 aatcttctga tacactgata gagtccaaat accattcgtt gggaaacgtt tctttatcaa
    11161 tgaacaaacg tgtagacgca tctagctgat atttctctaa gataggtacc agtggctgat
    11221 cgatgattga ttcttcttca tctgtataac cgtaagcaac tcctgcaact ttaccgtcta
    11281 tttcttctac gatacctcgt gaataactgt aacgataatt tggatcagtg attgcttctt
    11341 ctaaaatctc taaagtcttt tgttcccctg tctgcttcaa aaatggcagt tccatatctt
    11401 ttagaatcac taagatcaat ggggcgattt ctggagcatc ttcttttctc gcaaaacgaa
    11461 tcatagtttc ctctcctttt ctccattcta acatgcagaa aaagaaatcg caaaatcgtt
    11521 attttaaaaa atcttcaatc ttttcagtaa tgaaataacc atgagtaacg attcttgccg
    11581 cttcagttcg gcaataaggg cagacgactg gcatttcttt atcattatgt tcgactacat
    11641 tattgacttc ttcgtatgca aatttttttc cgcaattagg gcatttcatt ttttccatgt
    11701 tatctcaact tcctttcatc tttactctct tagcttactc ttatattgta aaaaagagaa
    11761 gcaatatcct tcttctatgc agatatccat acctgacggt cctgttctcc atctattaat
    11821 cggactttat aataaggacg ttcatctttg actagaagtg tccactcttt cacttttgct
    11881 tttttcccgt agtagctgac tgcttccttc atcgctgctt gcggttgttt gaactcttcc
    11941 gttttttcta tatcaaaaag atgttctgcg gccttttccg tgtcatttcg tacggttgtt
    12001 ttcccagtgg atggattgac tgctatgact tgtccttcac aaaaaaactg gtattcatat
    12061 gttgcattcg taggattctc caaatctttt tcaactgcaa aatgaatagc agatatgtta
    12121 tgttcttttg tttcgttttt tacgattgct gctgcttttt tcaatgagat ccgatattcc
    12181 tggtcactaa tatcatccat gtgaaaagcg gcattgagca gtcccgtcat accggtaatc
    12241 cctattacga tgagggcagc aacaccaaac atcatttttt tcattcttca ttctcctgtt
    12301 tctttttttt aaagaatatc aagaaatgaa cgaatgaaaa atcgaatgaa acgaaaaaaa
    12361 agtgacaatt ttgttatctt tattcgtgca gttctaatgg caatccgtct ggatcaaaga
    12421 aaaacgtcat tttttttcct gtaaaggtat cttctctaat cggttctgat tcgatcccta
    12481 aggactgcaa ttctgcaatt gtttcttcaa tatcgtctac tttgaaagca agatgacgca
    12541 agccacaagc ttctggatag tttggtcgtt gtggcgcatt agcaattcca aaaatctcca
    12601 attcagcact tcctagtttc agatctaatt tatagtcatc tcgttctttg cggtaatttt
    12661 ctctgattac ttcaaatcct aatttattaa cataaaaatc acgtgatttc tgataatctg
    12721 aaacaatgat ggctatatga tggatcgttg taaggttcat atgagcactc ctattcttcg
    12781 tttaagctcg gatctatatt aatctccagt tccattgtaa atgtgtcaga ttgaaaaaaa
    12841 tagctgtttt tcctttttat aagaaaaaag gcaaaaaaaa cagaaagtag gtcctccaag
    12901 acactcttcc tgttcccaat ataataaatg aaagtaagaa attatatttt caagaaaaca
    12961 aacagaaaat tatcataaca caaattattt ataattgaaa gactagtttt ttttcaagaa
    13021 aagcatacaa tagattgtgt tagtaaatac agaacacact acatttagtg tcttaaggag
    13081 tcctcataca tgaagtcata ttttcagttt ttagctcatc agctaaaagg ctggccgcaa
    13141 caaaattatt atcttttctt tttcagtctt ggctgccagg tcatgactct tgtcaatagt
    13201 cccatcactt ggctgtccgt tattacattt atcggtacca cgcttggtgt attgtgtatt
    13261 ttatccatca atgcggcaaa atcagtcaac ggtattttag gtattttgtc tgcgatttgt
    13321 tttattatcg ttgggttttc agcaaagaac tatttaagta tcggtgaaca gattgcttat
    13381 atgatcacat tggatatccc cgtattgtta agtaaagaat ggaatcataa tatggcttca
    13441 aaaatccgaa aattcactgg aaaaagctgg tttatcgcaa tcatcagtac tttccttgtc
    13501 tatcttgttt caggattcct gattggtcgt tatacagatg atcctcggcc gtgggtagat
    13561 gccattgctt tttctattag cttgactgct ggtatcatca gtttcttgcg ttacaacaac
    13621 caatattact ggtggttagc ttcagggctt gctcaaatgg tcctttggta tatttcattt
    13681 acgcatggat ctgcaagttt ggcgatgttc gtgaacagct ctgtttactt gctgaatgat
    13741 gtccttgcat ttaccgtttc accttggtat aaccaaaaag aacgtgaacg tatgcaaaaa
    13801 atggaagaag aatactacaa cgcacaacaa cacgcataaa aaaactgagc gtgggacaaa
    13861 agtaaagaac acttttgtct cacgctctaa acacgtataa acggcggaat cagaagcaac
    13921 tccttcggaa ataagctgaa attcacaaaa atttgaaaag caattttcgt gaatttcttc
    13981 ttatttcttg gagttaaacg cttctgtccc agcctctatt ttttatagta attccttcag
    14041 atactttctt gcctcatcat tgaaacgcag tgattcttct aacttctgat aatattccaa
    14101 cgcttgtttc tgagcttcag taaaacgata atgttttcct ttccatttgt cgattgtctc
    14161 ttgcacattt ccagattgga tatctttcac tactagttgc catttttctt ttgagactgt
    14221 acggtctttc ttatcagcag attgtatagc ttctcgttct ttacgcaata cttttaaaaa
    14281 accatttctg actatccgag gaatgctgtc gaagaaccct tctgtaaaac catagcgaca
    14341 atattgcttc caaataaata gatagatttt tggttggatg aatcgttggt ttttcgcaaa
    14401 atattccatc tgagcagtga cttctggtgt tcgttgacgt tttgtctttc tcaacacgga
    14461 aataaatgtt tcaaaataag cattaggatg tactggcact tcttcatata cgatggattg
    14521 ccaatcttta cgtaaaaatg cgcgatagtt gatcaagaat tcttctacat cctttactaa
    14581 ttcttctgca aagtcgtcaa atagttcttt ttttatctgt cgcttttttg actgcccatt
    14641 gatgtgttca cttattttct tgatttcatt ttccaatgct tgataatccg ctatgtaaat
    14701 cggcatttct acttggcgaa attctgcgag ccgctgagct aattttatat ccggtaaata
    14761 tggctgtttc atccgacgat tttggtacaa gacaaaacag tatttttgcc atagtccttc
    14821 aggaaaatcg atattcttct gcttaagcca taataattcg tctaaggcca gatcaacttt
    14881 tgtcattccc tgatggatcg aagctgccac tgtcggagag acatgcaggt gatcggcaaa
    14941 gtggttgatg cctagtttta ggatctttcc ggtttttggc gatttaacga tgtattgcac
    15001 ttttaattct ctaccacact cacaatacaa tttttgctgg cttcgttttg ttcgaaaaaa
    15061 cggattgatc ttgaagtcag aaaacaccca atctgttgca gctaaataat gttcttccat
    15121 aaaacgagag ctgattagat atcggcggtg cttatcgact agttcccgct gttttttcgt
    15181 taattttgca tagatctccc aacgttgact gcttgtgtat tgtttagaaa ttttgatggg
    15241 atagcttttg ctgatttctt ttggaaacca ttcttcaaag acggattgcc attcttccac
    15301 ggctgtttct ttcctttcta attacaaaaa gctggctact aacataacgt tagcaaccag
    15361 ccagtgtttc ttgctaaatt agtgagttgt tccggcattt ccggaaatat cttctttatt
    15421 ttctactgct gcttcaactg cataagtcaa agaagaagca atgtcttcta aagacataga
    15481 aggcgtaccc actggacgat cgatcacttg atcgggtgag aatggcacgt ggatgaaccc
    15541 acctcgaatt gccgggaatt gtgtttcgat caaatgaagc aaacgataca tgatatcatt
    15601 gcagacaaat gttcctgctg tataagagat gtacgctggt agaccatgat cttgtacatt
    15661 ttttgtcatt gctttgattg gcaatgaggt aaagtaagcg gctggaccgt tttcttctaa
    15721 agccgtatcg aatggctgat ttccttcatt atcagggatt cttgcttctg ctagattgat
    15781 tgcgacacgt tcgaacgaga ctgctgaccg cccacctgct tgtccgacac agatcacgac
    15841 atctggttgg tgctgttgga tggcttcttt gactacttga ctgctttttt cgaatacggt
    15901 cggtacttcc actttgatga tttcagcacc gctgatttca tctggcattt tttttactgc
    15961 ttcataggct ggattcactt tgtccccacc aaatggatca aaacctgtta ctagtacttt
    16021 catctgttgt tcctccgtcc gattgattta acatatggtt atcttttatc ataccatttt
    16081 ccttgaacag cgtaaataaa ggaaacaata gtttaaataa cataacagat caaacttaat
    16141 atattcttat aaaaaaatag tgttccacta caatgttaga agaggttgcg acaattattc
    16201 tgtcacaacc cctctcttct attatttaaa tagaatcata taagcaattt ggaaaactag
    16261 cataaataac gcaataaaca actggttttt gatcactccg tatttgtctt tcatctccag
    16321 catcgctaca ggcacgatat tgaagttagc agccatcggt gtcagcaggg tcccacaaaa
    16381 accgcaagtc aatgcaacca ttcctattaa cgctggattc gctccatgac taaatacgaa
    16441 tggtgcaccg atcccaaccg tcatcacggt gatggccgca aatgcattcc ccatgattac
    16501 cgtaaatatc accatgccaa gagcgtaaat gatgatgccg atgttcacat ttccttctgg
    16561 aacgatcgta cctactgcat tggaaatcac tgtcccaact ccagcgcttg taaataccgc
    16621 acctagagaa gctaaaagca ttggcaacat gcttaacggc cctactgtgc ccagcatatc
    16681 tgctgcatca tctaaaaatg tacttggtcg attatctcga gaaaagaaca tcaagattag
    16741 catggctgca aaaacaccga cgcccacgcc gactaaagca cctaaattgg taaatagagc
    16801 aaacaagatc gcaaagacac cgatactcaa agctggaata aaaattttca ttcctagttt
    16861 gtctgacatt ttttccatat attcttttga tggaagccgg ctttctcctt tgctgacttt
    16921 cttagcaatt gctggtatgg tcatcgcaaa gatcaagcca ccgttcacga ttgccggaag
    16981 ccatcttcct cctgcaataa ctacccctaa gaccgtccag aaaaaggctg acccataacg
    17041 atgaggattg gttttatctg ttaaatttct tactccagtg taaatcaata cgagtcccat
    17101 cacgatataa aggatctcca gtaatttatc gcctaataaa atatcaggat ttgtaaaaaa
    17161 gtccatctta ttgccccttt cctggataat atttcttcgc taatttcttt tctttaagat
    17221 agaagtagac acttgccaca actaaagccg tgatagccac tggaacttcc actaaagcaa
    17281 gctgtcccaa tgttacgtca tatcctagat ctttcagtgt tgattggact agcaacgccc
    17341 ctgctcctcc gatgaatagt acttggccaa agaaccagca gacattttcc atagctgaag
    17401 ccataccttt tagttcttct tcatgcttag gatggacttc taaccctttt gattcgatgg
    17461 tccccaatgc catcggtaaa atgattggac gaacaaatcc agcaactcca ccaaaactca
    17521 cgttaaaagc cgcaaagatc attcgaaaga tcccataaat atctacaatc ataccagcag
    17581 atactttctt gaatcgcttg atcaaggaag ccgctgattc tttcagacca tttctttcaa
    17641 gcgttcctgt tgccaacata atgatgataa aaatcgccat cccgcgattg tttacaaaac
    17701 ttgtacctaa aagttctaac attccttgaa tgccaagacc gccagtcaaa gctgtaacaa
    17761 ctaaagcaac cattacgatc aatatcgagt ctaatttaaa tgcaaacccg agaataacaa
    17821 tcaagacgcc taaaaggcta agataatttt ccatatattt tcctccataa a
//
LOCUS       DAZKLD010000050        17591 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.050, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000050 DAZKLD010000000
VERSION     DAZKLD010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17591
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.050"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(167..1114)
                     /locus_tag="ACG8KR_001391"
     CDS             complement(167..1114)
                     /locus_tag="ACG8KR_001391"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFQ0200769.1"
                     /translation="MKVSIVVPCFNEEKTVPLFFDEVEKFKGAHEFEYLFIDDGSKDG
                     TLAAIKKLASAYPYVRFVSFSRNFGKEAALYAGLQASTGDLVTVMDVDLQDPPELLPE
                     MINRITTSDIDCVGTRRSTREGEPAIRSFFAKKFYQLINKISDTEMVDGARDFRLMTR
                     QMVDAVLELTEYNRFSKGLFSWVGFKTEYISYENRERVAGETSWSFWKLFNYSIDGIV
                     NFSDAPLTIASFIGALSCVGSGIAILFIILRALLFGDPTSGWPSMVSIFLFIGGIQLL
                     CLGIIGKYIGKIFLETKQRPVYIIRETEKVKPNELKPHQ"
     gene            1360..1824
                     /locus_tag="ACG8KR_001392"
     CDS             1360..1824
                     /locus_tag="ACG8KR_001392"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HFQ0200770.1"
                     /translation="MKIHTPIGFLWLDADESGLTAVSFQPISESESANQRILTRTQEQ
                     FEEYFAGKRKEFDVPLSIKRGTPFQQNVWKALSTIPYGEIRTYKEVAVAVDSPKAVRA
                     IGQANRTNPLPIVIPCHRVIGQNGKLTGYMGNAIDGVEIKRQLLLLEGYLQN"
     gene            complement(1886..2929)
                     /locus_tag="ACG8KR_001393"
     CDS             complement(1886..2929)
                     /locus_tag="ACG8KR_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HFQ0200771.1"
                     /translation="MKSLSHPIKSVKDSAVREILPGLLLSFAIAAFSRLLAIYLPQLG
                     GATIAILLGILLGNTFFKQPVLAIGTKFAESKLLEFSVFLLGATVTAQTIAQIGVKGI
                     VFVLLQMSVTIIGAYLIGKKLQFSDTSSLMMAGGNAVCGSSAIASIAPSIHAKEEEKG
                     QIITLVNLLGTVMMLTLPFLGLQLFGGNLIEKSALLGGTLQSVGQVVAGASLLDNSVV
                     EFAMLFKILRIMMLVVVVYLFEKFMHKKQPALFETPTDTQKKRKLPLPWYVLGFVIFC
                     LVNSLFSLPVVIDQSAHFISSWFEITALAAIGLRLDFAKFMKEGTRFLSYGLAVGLLQ
                     TVMALLFIWLIKV"
     gene            complement(3306..4271)
                     /locus_tag="ACG8KR_001394"
     CDS             complement(3306..4271)
                     /locus_tag="ACG8KR_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HFQ0200772.1"
                     /translation="MKNFLKIIPIPICGLILGLASLGNLFKDYHHVALGNLTGGISMI
                     LMIFIFVKLFILFEHTKQTLHDPIIASVSPTFTMSLMVICTYFVSYESIAPIVKFIWL
                     CAVIFQVILVFYFNYHHVIKADLSIEAIYPSWFIIYVGFGVITVTAGNFFPLIGKIFF
                     WISLICYAFLLPIIIKRVFFVRNMAHPTRPLITIVAAPGSLCLTGYLKNFAEPNFYLV
                     VALFALSQVLYFVVLSMLPRLFKLRFYPSYAAFTFPLVISATAIFTTVHYFNSLGIYS
                     SVLDFLKVAECLFASVVVFYVLAHYVRFLIKEHSKHGKFVSKKIA"
     gene            4505..5350
                     /locus_tag="ACG8KR_001395"
     CDS             4505..5350
                     /locus_tag="ACG8KR_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFQ0200773.1"
                     /translation="MFKLLTTFRAVYETRNFSRAAEVLFLSQPAVSNQIKQLERELNI
                     DLFQRNGRKEMLPTKQADLLYERTLVLLEEWEETQQKLLNEQNEEEVCRIVASHTFAV
                     YLLPQLMKHLIKRFPKVHFEIEITNSHESLEQVAKHESDFGFIEKPLETSGVQRYSLM
                     EDQLVIAGDPKSKLWLVREANSGVFHYTQRYFEEMNIQDKKMIVKSNDVIVALLKEGI
                     GRSILSKRSVPENVPMTPLNFHRYFYFIQRTNLVSDQLLEIAEEILRYNESTETSHSE
                     ENLSW"
     gene            5541..6728
                     /locus_tag="ACG8KR_001396"
     CDS             5541..6728
                     /locus_tag="ACG8KR_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HFQ0200774.1"
                     /translation="MEKKLKASNDNRIDYGVILPVFLLCLIGLLSLYVALSHDPNNPA
                     VLKGVGMQVVWYLVGAAAIVVIMHLNSKWIWKLTPYLYGLGLVVMGLLLKFYDQNLAA
                     STGSKNWFRFGAFTFQPAELMKIAYILMMALIVTKHNTQVKERTMKSDFWLIGKLLIV
                     TIPVLALIMAQDDFGTMLVFLAIFGGIFLMSGISWRIIAPVVILAVVVGAGTIFLVTT
                     EGGRDLLYKVGFKSYQFARIDSWLDPFHDTSGMSYQPAQGLLAIGTGGLFGKGFNVSN
                     IYVPVRESDMIFTVIGENFGFIGGAFVIFLYFILIYRMIRVCFDTNNEFYAYIASGLI
                     MMLLFHVFENIGANIGLLPLTGIPLPFISQGGSSILGNMIGIGLILSMRYQNEAPARS
                     SRR"
     gene            6734..7090
                     /locus_tag="ACG8KR_001397"
     CDS             6734..7090
                     /locus_tag="ACG8KR_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HFQ0200775.1"
                     /translation="MYTFYWYPKCSTCKKAKAWLDQHGVSYQTVDMIKEVPKASTIET
                     WLETSQVPIRRLFNTSGMKYRELGLKDQVGEFTASKASQVLSTDGMLIKRPLILREDQ
                     LVAIGFNEKIYEGELI"
     gene            7092..7430
                     /locus_tag="ACG8KR_001398"
     CDS             7092..7430
                     /locus_tag="ACG8KR_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HFQ0200776.1"
                     /translation="MAGKHLKKKDNLWILFNGKEYCVGLTNEAQEELGDITFANLPKA
                     GQSLKKDEPLIEVEAEKAVNEFASPLTGTVSSVNDKIGEDINILNDSDEMNAWILSFK
                     DVDPKEFDEL"
     misc_binding    7506..7762
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            7825..8859
                     /locus_tag="ACG8KR_001399"
     CDS             7825..8859
                     /locus_tag="ACG8KR_001399"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HFQ0200777.1"
                     /translation="MSLIQLTNVKKTFNGKNGNIQAVNDVSLNVEKGDIYGIVGYSGA
                     GKSTLVRLLNGLELPTSGEVIVNDQDITKLKNKELRTFRKKIGMIFQHFNLLWSRTVL
                     ENIQLPLELAGVPKSKRKERAEELLRLVGLEGRGQAYPSQLSGGQKQRVGIARALAND
                     PEILLCDEATSALDPQTTEEVLDLLLAINKKLNLTIVLITHEMNVIRKICNKVAVMEL
                     GKVVEEGDVLTVFRQPKKEVTKRFVQQDIEPEADSTELLAELIKENPSGRLATLTFSE
                     RNANEPVISQAIRRYNIDVNVVYGKIKQVKEGSFGSLTVLMTGETKELDQAEVFIKEQ
                     GVGIEVIHRG"
     gene            8852..9538
                     /locus_tag="ACG8KR_001400"
     CDS             8852..9538
                     /locus_tag="ACG8KR_001400"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease"
                     /protein_id="HFQ0200778.1"
                     /translation="MDKSFSETYLDFKQINPETMNQAILDTLYMTLISMVLVTIIGLV
                     LGLILYSIGRKNKPYARIIYGIVSIISNIFRSTPFMILMVLIIPFTKAIVGTMLGAQA
                     AIPALVLSAAPFYARLVEIAFREVSPGVLEAADAMGASYWEIIWKVLIPESIPALISG
                     LTVTTISMIGFTAMAGAIGAGGLGGLAWQEGYQRGNLTVTFVATLIILLIVFIVQGIG
                     DFLTKKTDKR"
     gene            9562..10410
                     /locus_tag="ACG8KR_001401"
     CDS             9562..10410
                     /locus_tag="ACG8KR_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HFQ0200779.1"
                     /translation="MKKKIFGFAAALLLTVGLAACGNNSDSKDSANKADDTTLKVGAS
                     PTPHAEILEHVKPLLKDEGIDLEIVKFDDYVLPNQSLEEGDIDANYFQHIPYLNKEIK
                     EKGYDFVNAGAVHIEPMGLYSKKVKDVSELKDGATIITSNSESDWGRIITILQDAGLV
                     KVKDGVDLETATFDDIEENPKNLKFNHTIDPSLLASTYQNDEGDLVAINANFAYGAGL
                     NPLEDAVLLEKDNSPYVNIIATRKGDEDSDKIKKLIEVLHRKEVQEWITDKWGGSVKP
                     VKADAK"
     gene            10607..11380
                     /gene="sufC"
                     /locus_tag="ACG8KR_001402"
     CDS             10607..11380
                     /gene="sufC"
                     /locus_tag="ACG8KR_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HFQ0200780.1"
                     /translation="MSVLEIKNLHVSIEEKEILKGVNLTMKTGEIHAIMGPNGTGKST
                     LSAAIMGNPNYEVTEGEILFDGKNVLDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAINAKRDENDKISVMAFLKKLDEKMALLNMPEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFAILDEIDSGLDIDALKVVAKGVNEMRGDNFGALIITHYQRLLNYITPDVVHI
                     MMEGRVVMTGNADLAKRLEAEGYAGISKELGIDYKEEEA"
     gene            11393..12679
                     /gene="sufD"
                     /locus_tag="ACG8KR_001403"
     CDS             11393..12679
                     /gene="sufD"
                     /locus_tag="ACG8KR_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HFQ0200781.1"
                     /translation="MKEKNWLDYLDAVNDFSLSKGEPDWMRTFRQDALAKADELPLPH
                     IDRVKFHRWSLFDVKETQTISETGTIPAFDAMKDNPVLVQQGSWTIFEQLPVELAEKG
                     VIFTDLFTAMIEYPELVQEYYMKKAVNMNEDQLTALHVAFMNSGIFLYVPKNVVIDEP
                     LESLFIQDGASDEHFFKHVLIVADEHSEFSYLERFQTTKEQVAKSSGNIIVEVIAKAG
                     SKIKYSAVDQLGENITSYMNRRGHILRDASVDWAIGVMNDGHVIADFDSDLAGEGAHA
                     EVKIVAISSGRQIQGIDTRVTNKAPHTIGHILQHGVIREKGTLTFNGIGHILKGAKGA
                     DAQQESRVLMLSDKARGDANPILLIDENEVTAGHAASVGRVDPEEMYYLMSRGLHKEE
                     AERLVIRGFLGSVLTAIPVEQVRKELVEVIEGKLNG"
     gene            12676..13911
                     /locus_tag="ACG8KR_001404"
     CDS             12676..13911
                     /locus_tag="ACG8KR_001404"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HFQ0200782.1"
                     /translation="MIDAKKLRNDFPILFQDVNDEPLVYLDNAATTQKPKQVLQALAY
                     YYEHDNANVHRGVHTLAERATHDYEQAREKVRAFIHAKETAEVLFTRGTTTSLNWVAR
                     TFGDRYIKAGDEIVLSYMEHHSNVIPWQQLAKRTGAVLKYIELTEEGFLDMEDAAKKI
                     TDKTKIVSIAHVSNVLGVFNPVQELAELVHENGGVIVVDGAQSAPHMAIDVQALDCDF
                     FAFSGHKMCGPTGIGVLYGKRKWLEEMEPVEFGGEMIDFVELYDSTWKELPWKFEAGT
                     PNIAGAIALGHAIDYLQKLGMDNIHRYEEELAAYVLPKLQAIDGLTVYGPQDPNFRTG
                     VVAFNLDGLHPHDVATALDMEGVAVRAGHHCAQPLLKYLEVSATARASFYFYNTTQDA
                     DRLVEAIMATKEFFQHGTI"
     gene            13898..14368
                     /gene="sufU"
                     /locus_tag="ACG8KR_001405"
     CDS             13898..14368
                     /gene="sufU"
                     /locus_tag="ACG8KR_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly sulfur transfer protein
                     SufU"
                     /protein_id="HFQ0200783.1"
                     /translation="MGLSKLDNLYRQVILDHSSHPHHRGSLTDSTSKIELNNPTCGDV
                     IELQVDVKDGIIKNIAFDGSGCSISTASASMMTDAVIGKSIEEAEHLTVDFLQLVQGK
                     EVKDPDQLGDAAMLGGVAKFPARIKCATLAWKALGKAMENDGTAAFHHYHKEEE"
     gene            14373..15764
                     /gene="sufB"
                     /locus_tag="ACG8KR_001406"
     CDS             14373..15764
                     /gene="sufB"
                     /locus_tag="ACG8KR_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HFQ0200784.1"
                     /translation="MGVPELEEYKFGFHDDVEPVFSTGEGLTEEVIREMSRIKGEPEW
                     MLEFRLKSLETFNKMPMQTWGPDLSDIDFDAIKYYQKPSDKPARDWEDVPEKIKDTFE
                     KIGIPEAERAYLAGASAQYESEVVYHNMKDEFEKLGIVFTDTDSALKEYPELFKEYFS
                     KLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDVPLQTYFRINAENTGQFERTLIIVD
                     EGASIHYVEGCTAPTYSSNSLHAAIVEIFTRKDAYCRYTTIQNWSDNVYNLVTKRAKA
                     YEGATVEWIDGNLGAKTTMKYPSVYLDGKGARGTMLSIAFAGANQIQDTGAKMIHNAP
                     NTSSSIVSKSISKDGGEVNYRGQVTFAKNSAGSISHIECDTIIMDEWSKSDTIPFNEI
                     HNSQVSLEHEAKVSKISEEQLYYLMSRGLTESEATEMIVMGFVEPFTKELPMEYAVEL
                     NRLISYEMEGSVG"
     gene            complement(15859..16374)
                     /locus_tag="ACG8KR_001407"
                     /pseudo
     CDS             complement(15859..16374)
                     /locus_tag="ACG8KR_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301919.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
     gene            16452..17578
                     /locus_tag="ACG8KR_001408"
     CDS             join(16452..16680,16680..17578)
                     /locus_tag="ACG8KR_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611172.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFQ0200785.1"
                     /translation="MTRYEENFKQMIVELNQTGRSVRGLAKEYGLSEATIYKWKNLYL
                     PDQSTGLTGKEVAELRKENARLNEELEILKKAGSHILSENLNSLVQFIEKWCKDYTVS
                     LLCRLLEIPRSVYYFYKNKPLTATEIRNNKLKKKISTIFFTNKQRYGATKIHQVLLKE
                     GISVSLKHVQKLMKQLNLRSIVVKKYRPQRSNKPIISKENLLNQDFSTETICEKWAAD
                     ITYIPTKKNGWCYLSSIMDLHTKKIISYTFSKRMTVDCVIQTLNKAKIHYHIPEGMIL
                     HTDLGSQYTAREVEQWLKTNKIRHSYSRKGTPYDNAGIESFHASLKKEEVYTTSYSDF
                     EEANRALFSYIEGFYNRNRIHSSIHYLTPQEFEELAKEKMA"
BASE COUNT         5854 a         3277 c         3705 g         4755 t
ORIGIN      
        1 ataagaagga atttacgaaa attgcttctc aaatttttgt gaaatttcgg cttatttccg
       61 aagaagctac ttctggaaca ccatttattc ggaaaaaagg actgtgacaa gacttttgtc
      121 acagtccttt ttcatttaaa aaaatgttta cttcatttga cgtatattat tggtgaggct
      181 tcaattcatt tggcttcact ttttctgttt ctcttatgat ataaacagga cgctgtttcg
      241 tttctaagaa gattttccca atatatttcc cgatgattcc aagacataac aattggattc
      301 caccaatgaa caggaagatc gagaccatgg aaggccatcc tgatgtagga tcaccaaaca
      361 acaatgcccg caaaataatg aacaaaattg caataccaga accgacacaa gacaaagcgc
      421 cgataaatga agcaatcgtt aacggtgcat ccgagaagtt cacgatacca tcaatcgagt
      481 agttgaataa tttccagaag gaccaagatg tttcgccagc cacacgttcg cggttttcat
      541 aagaaatata ttctgtttta aatccgaccc agctaaataa tccttttgaa aatcggttgt
      601 attctgttaa ttctaggacg gcatccacca tttgacgagt catcaagcgg aaatcacgag
      661 cgccatcgac catttctgta tcgctgattt tattgatcaa ctggtagaat tttttcgcaa
      721 agaaactgcg tatcgcaggc tcgccttcac gagtcgaacg ccgggtgccg acacaatcga
      781 tatcagaagt agtgatccgg ttgatcatct caggaagcag ttcgggcgga tcttgcagat
      841 caacatccat cacggtcact aaatctcctg tagaagcttg caatccagcg tacagagccg
      901 cttctttccc aaaatttctg gaaaatgaga caaagcggac ataaggataa gctgaggcta
      961 gcttcttgat tgctgctaac gtaccgtctt ttgatccgtc gtcaataaac aaatattcga
     1021 actcatgggc acctttgaat ttttcaacct catcaaaaaa aagaggaaca gttttttctt
     1081 cattaaaaca tgggacaaca attgatactt tcatcgtttc tatcctttca atcgtatgtt
     1141 tatatatggt ttacattcaa aatttaataa accttaaata acacctttaa aatttgattt
     1201 tacgtaaaag aacaggtatt ctttttgtaa actaacgtat ttagtttacc atgtttcctc
     1261 ggtttatcaa acttttaaga acaattttta ttagttggca agtagttttg gatagagaaa
     1321 aaggaggaag ttccagtcta ttgactagga gcaaacaaaa tgaaaataca cacacctatc
     1381 ggctttttat ggttagatgc agatgaatcc ggattgactg ctgtatcttt tcagccaatt
     1441 tcagaaagcg aatcagcgaa tcagcgaatt ttaacgcgca cacaagaaca gtttgaagaa
     1501 tattttgcgg gaaaaagaaa agaatttgat gttcctttat ctataaagag aggtacgcct
     1561 tttcagcaaa atgtctggaa agctttgtcg accattcctt atggagaaat ccgaacgtat
     1621 aaagaagtag ccgttgctgt tgatagtccg aaagctgtgc gagcgattgg gcaggcaaat
     1681 cgaaccaatc cattgccaat cgttattcct tgtcatcgag tgatcgggca aaatggaaaa
     1741 ctgacaggat atatgggaaa cgcaatagat ggggttgaaa tcaaacgcca attgttgttg
     1801 ttagaaggat atctgcaaaa ttaaaaacag ttgtgattcg gacattgttc catgcgagaa
     1861 tcacaactgt tttttcatgt tcattctaaa ctttgatcag ccaaataaat agtaaagcca
     1921 tcactgtttg gagaagcccg acagctaatc cataagataa gaagcgtgta ccttctttca
     1981 tgaattttgc aaagtctaaa cgcagaccaa ttgccgctaa agcagtgatc tcaaaccagc
     2041 tgctgataaa atgtgccgat tggtcaataa ctacaggtaa agaaaacaag ctgttgacca
     2101 aacaaaagat cacaaaccct aatacatacc aaggaagcgg taattttcgt tttttctgcg
     2161 tatcagttgg cgtttcaaac agagctggtt gtttcttgtg catgaatttt tcaaacagat
     2221 agacaacaac aacgagcatc ataatacgta gaatcttaaa tagcatggcg aattcgacaa
     2281 cagaattgtc aagtaagctt gccccagcga ctacttgacc aacagattgg agtgtaccac
     2341 ccaaaagtgc acttttttca atgagatttc caccaaacaa ctgtaatcca agaaaaggga
     2401 gagtcaacat catcacagta cccagtagat tgactagtgt gatgatctgt cctttttctt
     2461 cctctttcgc atggatggaa ggggcgattg aagcaatagc agaagaacca caaacagcgt
     2521 ttcctccagc catcatcaaa gaagaagtat cgctgaattg cagtttttta ccaatcaagt
     2581 aagcaccgat gatagtgact gacatttgca gtaacacaaa aacaattcct tttacaccaa
     2641 tttgagcaat cgtttgagct gtgacagttg cacctagtaa aaaaacagag aattctaata
     2701 gtttgctttc agcaaatttt gtgccgattg ctagaacagg ctgtttaaaa aaagtatttc
     2761 ctaataaaat acccaataaa atagcaatag tcgctccacc taactgtggc agataaatag
     2821 caagaagcct gctgaaggca gcgatggcaa aagaaagcag taaaccgggt aagatttcgc
     2881 ggaccgctga atcctttaca gattttattg gatgtgacaa tgatttcatt tgatcggttc
     2941 ctttcgtcaa taaatactaa aagcatactc gaacatagta gcactaaacc agtattttga
     3001 caaaagaaca agaggtcgtg acaaaagtct tgtacgacct cttgtttcga ataaacggtg
     3061 ttccagaagt agcttctacg gaaataagcc gaaatttcac aaaaatttga gaagcaattt
     3121 tcgtaaattc cttcttattt cttgaagctg accacttctt ttacgacctc ttggtaaacg
     3181 gtgttcaaaa gcttgcatct gcggtaataa gcccaaccag tacaaaaaca tgaacagctg
     3241 tttttgtttg gccgttctta ttacttgatg cagaacagct ttttcacaac ctattgaatc
     3301 gattattaag cgattttttt agaaacaaat tttccatgtt tgctatgttc tttaattaag
     3361 aaacgtacgt aatgagcaag aacgtagaag acaactacac ttgcaaacag acattccgca
     3421 acttttaaga aatccaatac gctactatag atacctaaac tgttgaagta atgaacagta
     3481 gtaaatatag ctgttgcaga gataacaagc gggaatgtaa aggctgcata gcttggataa
     3541 aaacgaagct tgaacaaacg tggaagcata gacagcacaa cgaaatacaa tacttgagat
     3601 aaagcgaaca atgctacgac aagataaaaa ttaggttcag caaagttttt aagataacct
     3661 gttaggcaaa gagaaccagg tgctgcaacg atcgtaatca gcggacgtgt cggatgagcc
     3721 atatttctta cgaaaaagac acgtttgatg atgatcggca ataagaacgc atagcagatc
     3781 aatgaaatcc aaaagaaaat ttttccaata agtgggaaga agttcccggc agttacagtg
     3841 atgaccccaa atccaacgta aatgataaac cagcttggat aaattgcttc aattgaaaga
     3901 tcagctttga taacatggtg ataattgaaa taaaagacaa ggatcacttg gaagataaca
     3961 gcgcacagcc aaatgaattt gacaattggt gcaatacttt catatgaaac aaaataagta
     4021 caaataacca tgagtgacat cgtaaaagtt ggtgaaacgg aggcgatgat tggatcatgc
     4081 aacgtttgtt tcgtgtgttc gaataagata aacaatttaa caaaaatgaa tatcataaga
     4141 atcatagaga taccgccagt tagattgcct aaagcaacat ggtgatagtc tttaaacaga
     4201 tttccaaggg aagctaaccc aaggatcaac ccgcagatcg ggatcggaat aattttcaaa
     4261 aagttcttca tatataatat tctccttttt atttaataga ttttagtacc aagatgaacg
     4321 aatacattgt aaaatgaata ggaatgtatg attacgagca agcagacagc tgattgtctt
     4381 catctaagtt actagttgga tcagcaactg ttttgttgta catttctatt atagatggtt
     4441 ttttgtatat ttaaatcaaa tagtttttat agtaattatc ataaaaagtt atggaagtgg
     4501 tgccatgttt aagttattaa cgacttttcg agcagtatat gaaacaagaa atttttccag
     4561 agcagcagaa gtactgtttt tatctcaacc agcagtctcg aatcaaatca aacagttgga
     4621 gcgggaattg aatatcgatc tttttcaacg taacggaaga aaagagatgc ttccaactaa
     4681 gcaagccgac ttattatatg aacggacact ggttttgtta gaagaatggg aagaaactca
     4741 gcaaaaattg ctcaatgaac aaaatgaaga agaagtatgt cggatcgttg cttcccacac
     4801 ctttgctgtc tatcttttgc ctcagttgat gaaacatctg atcaaaaggt ttcctaaagt
     4861 gcattttgag atcgaaatca cgaattctca tgaatcatta gaacaagtag cgaaacatga
     4921 atccgatttt ggttttatcg aaaagccatt agagacttcc ggcgtacaaa gatactcttt
     4981 gatggaagac cagctagtca tcgctgggga cccaaaaagc aaattgtggc ttgtaagaga
     5041 agcaaactct ggtgtttttc attacacgca gcgttatttt gaagagatga atatccaaga
     5101 taagaagatg atcgtgaaaa gtaacgatgt aattgttgcg ttgctgaagg aaggaatcgg
     5161 tcgctcgatt ttgtctaagc gctctgttcc agaaaatgtg ccaatgactc cgttgaattt
     5221 ccatcgatat ttttatttca tccaacgaac caatctggtt tctgatcagc tgctagagat
     5281 tgccgaagag atccttcgat acaatgaaag cacagaaact tctcacagcg aggagaatct
     5341 cagctggtag ataattgcat gtatcaaaaa aagttctatg atacactaaa gataagcagt
     5401 aggcttttgt actgcatttg ctcaagcaac tgcacaaacg gaaagttaat ttttcccaaa
     5461 tgtctatgga aagactttcc attatctaac tggaccgcta taataatcag cagtatggtt
     5521 tttaagaaaa gggatgaaac atggaaaaaa aattaaaagc ttcgaacgat aatcgtattg
     5581 attatggcgt gattttacca gtctttctgt tatgtttgat tggtctgctc tcattatatg
     5641 tagcacttag ccatgatccg aacaatccag cagtcttgaa aggcgtaggg atgcaagttg
     5701 tctggtattt agttggagcg gcagcaatcg ttgtcatcat gcatttgaat tccaaatgga
     5761 tctggaaact gactccttac ctttatggtt tggggttagt ggtaatggga ctcttgctca
     5821 aattctatga tcagaattta gctgcttcaa cagggtctaa aaactggttt cgattcggtg
     5881 cgtttacttt ccagccagca gagttgatga agattgcgta catcttgatg atggcactga
     5941 tcgtaactaa acataatacg caagtaaaag aacggacgat gaaatcggac ttttggctga
     6001 ttgggaagct cttgatcgtg acaataccgg ttttagcatt gatcatggcc caagatgact
     6061 ttggaacgat gcttgtcttt cttgcgatct ttggcgggat tttcctgatg tctggaatct
     6121 cttggcgcat catcgcacct gtagtaatct tggcagtagt cgttggagca ggaacgatct
     6181 tcttggttac gactgaaggc ggaagagact tgctttataa agttggattc aaatcttatc
     6241 agtttgcacg tattgattca tggctggacc ctttccatga tacgtcagga atgagttatc
     6301 agccggccca aggacttttg gcaattggta ccggtggctt gtttggtaaa ggattcaacg
     6361 taagcaatat ctatgtgcct gttcgtgagt ccgatatgat ttttaccgtt atcggtgaaa
     6421 acttcggctt tatcggtggt gcattcgtca ttttcttgta ttttatttta atctatcgta
     6481 tgattcgggt gtgtttcgat acgaataatg aattttacgc ctatatcgca agcggtttga
     6541 tcatgatgct gctgttccat gtctttgaga atattggagc aaatatcgga ttacttcctt
     6601 taacagggat cccattgcct ttcattagtc agggaggatc ctcgatttta gggaacatga
     6661 tcggtatcgg gttgatatta tcgatgagat accaaaatga agcacctgca agatcttcaa
     6721 ggaggtaaga aaattgtaca ccttttactg gtacccgaaa tgttcaacat gcaaaaaagc
     6781 caaagcgtgg ttagaccagc atggcgttag ttatcaaacc gttgatatga tcaaagaagt
     6841 accgaaggcc tcaacgattg agacttggct agaaacgagt caagtaccaa tccgtcgcct
     6901 gttcaacaca agcgggatga aatatcggga acttggactg aaagatcaag taggcgagtt
     6961 tacggcttca aaagcgagcc aagtcttatc gacagatggt atgctgatca agcgaccact
     7021 gatcttacga gaggaccagc ttgttgcaat tggattcaat gaaaaaattt atgaaggaga
     7081 actgatataa aatggcaggc aagcatttga agaaaaagga caacctttgg atcttattta
     7141 atggaaaaga atactgtgtt ggtttgacaa atgaagcgca agaagaactt ggagacatca
     7201 cgtttgccaa tctgccaaaa gcaggacaat cgctaaaaaa agatgaacct ctgatcgaag
     7261 ttgaagcgga aaaagcggtc aatgaatttg ccagcccgct aacgggtaca gtttcttctg
     7321 ttaatgataa aatcggtgaa gatatcaata tcttgaatga ttcagatgaa atgaatgcgt
     7381 ggatcttaag tttcaaagat gttgatccaa aagaatttga cgaattataa aatcagaaaa
     7441 tcacttgaaa aaagttgaca ctgattcttc cagttgctat aatcataagc aatcctatat
     7501 gaaacgagaa agctttgaaa agaagagtac atgtcacgga cgtttgaaga gagccctgtt
     7561 tgctgaaaat gggtaacgaa aatgagatgg aagatggtct tagagcagag taagcgagcg
     7621 taagcaaact tactacggga gtgcccgtta cagcgcgaca gtatacggaa agaagctaag
     7681 tccaagtact ggaagaggct gttattgcga agtaacggta aatcaaggtg gtaacacgga
     7741 agttcaagct ttcgtccttt tgcttaaggc aagagggcga aggctttttt gtttgaaaaa
     7801 ttataagaga aagatgtgaa tggcatgtca ttaatccaac taacgaatgt caaaaagaca
     7861 ttcaatggga aaaacggaaa catccaagcc gtcaatgacg tttcactaaa cgttgaaaaa
     7921 ggggatattt atggaatcgt cggctattct ggcgcaggaa aaagtacatt agtccgtctc
     7981 ctgaatggac ttgagcttcc cacgagcggc gaagtgatcg taaacgatca agatattaca
     8041 aaattaaaaa acaaagaatt acgtacattc cgaaagaaaa tcggtatgat cttccagcat
     8101 ttcaacctgt tatggtcaag aactgtactt gaaaatatcc agctgccgct ggaattagct
     8161 ggcgtaccaa agagcaaacg taaagaacgg gcagaagaac tgttgcgttt agtcgggtta
     8221 gaaggacgcg gacaagcata tccttcacaa ctttccggcg gacagaaaca acgcgtcggt
     8281 atcgcacgag cattggcaaa tgatcctgag atcctgcttt gtgatgaggc tactagcgca
     8341 ttggacccgc aaacaacaga agaagtatta gacttattgt tagcgatcaa taagaaattg
     8401 aatttgacca tcgtcttgat cacacatgaa atgaatgtga tccgcaaaat ctgcaacaaa
     8461 gtcgctgtga tggagttggg gaaagtcgta gaagaaggag acgtattgac tgtcttcaga
     8521 caaccgaaaa aagaagtaac caaacgcttt gtacaacaag acatcgaacc agaagctgat
     8581 tcgacagaac tattagctga actcatcaaa gaaaatccaa gcggccgttt ggcaactttg
     8641 acctttagcg aaagaaatgc caacgagcca gtcatttccc aagcgattcg acgctataac
     8701 atcgatgtca acgttgtgta tggaaagatc aaacaagtga aagaaggttc ctttggttca
     8761 ttgactgtct tgatgactgg tgaaaccaaa gaattagatc aagcagaagt gttcatcaaa
     8821 gaacaaggag taggaattga ggtgatccat cgtggataaa agcttttcag aaacatatct
     8881 agattttaaa caaatcaacc cggaaacgat gaatcaggct atccttgata cactatatat
     8941 gacattaatc tcaatggtct tagtgacgat catcggatta gttttgggat tgatccttta
     9001 ctctattgga cgcaaaaaca aaccgtatgc tcgaatcatt tatggaatcg tatcgatcat
     9061 cagtaatatt ttccggtcaa cgccatttat gatcctgatg gttttgatca ttccatttac
     9121 taaagcaatc gtaggcacta tgctaggtgc gcaagcagcc attccagctc ttgttttatc
     9181 cgcagcacca ttttatgccc gtctagtaga gattgctttt cgtgaagtca gccctggtgt
     9241 actggaagca gcagatgcaa tgggagcaag ttactgggaa atcatctgga aggtcttgat
     9301 acctgaaagt attcccgcat tgatttctgg attaacagtt acaacgatct cgatgatcgg
     9361 atttactgcg atggccggcg cgatcggcgc aggtggactt ggaggtcttg cttggcagga
     9421 aggctaccag cgtggtaatc taacagtgac attcgttgcg actttaatta ttttactaat
     9481 cgtatttatc gttcaaggaa tcggtgattt cttaactaag aaaacagaca aaagataata
     9541 ggatataggg ggatgtagga tatgaaaaag aaaatttttg gatttgcagc agcgcttttg
     9601 ctgactgtag gattagcagc atgcggtaac aacagcgaca gcaaagattc agcaaataaa
     9661 gcagacgaca cgacattgaa agtcggcgct tcaccgacac cgcacgcaga gattttggaa
     9721 catgtgaaac cattattgaa agatgaagga atcgatcttg aaatcgtcaa attcgatgac
     9781 tatgtattac cgaaccaatc tttggaagaa ggcgacattg atgccaatta cttccagcat
     9841 attccttatt tgaacaaaga aatcaaagaa aaaggatatg actttgtcaa tgccggtgct
     9901 gtccacatcg aaccaatggg cttgtactct aaaaaagtca aagatgtttc cgaattaaaa
     9961 gatggtgcaa caatcattac ttcaaattct gaatcagact ggggacgcat catcacaatt
    10021 ctacaagatg ctggcttagt caaagtcaaa gatggagtag atctggaaac agcaacattc
    10081 gatgatattg aagaaaaccc taagaacttg aagttcaacc atacgatcga cccatcactt
    10141 ttagcttcta cgtaccaaaa tgatgaaggt gacctagttg caatcaacgc aaacttcgct
    10201 tacggcgcag ggttgaatcc attagaagat gccgtattgc ttgaaaagga caactctcct
    10261 tacgtgaata ttattgcgac tcgtaaaggt gatgaagaca gtgacaaaat caaaaaactg
    10321 attgaagtgc ttcaccgcaa agaagtccaa gaatggatca cagacaaatg gggcggctct
    10381 gttaaaccag taaaggctga tgcaaaataa ggtatggaac aaaaaagatg agaaataagt
    10441 ctgtaatggc ttatttctca tcttttttat acctattcta atttagaaat caaaattgtt
    10501 taataataaa taaaagatat ttgattttag taaggaaggt cattgcgctt cattctcaat
    10561 tagtctaaaa tgagtgagaa cgaaagagaa tttgggggta taatgcatgt ctgtcttaga
    10621 aataaaaaat ctacatgtgt cgatcgagga aaaagaaatc cttaaaggcg taaacctaac
    10681 aatgaaaacc ggagaaatcc atgccatcat gggacctaac ggcacaggaa aatctacctt
    10741 atctgccgct atcatgggca atccaaacta tgaagtaaca gaaggcgaaa ttttgtttga
    10801 cgggaaaaat gttttagact tggaagtaga tgaacgtgcg cgacttggct tatttttagc
    10861 tatgcaatat ccaagtgaga taccaggtat cacgaatgcg gaattcatgc gtgcagcgat
    10921 caatgcgaaa cgtgatgaaa acgacaaaat ttctgttatg gcatttttga aaaagttaga
    10981 tgaaaaaatg gctttactaa atatgccaga agaaatggct gaacgttatc tgaacgaagg
    11041 tttttctggc ggagaaaaga aacggaatga gatcctgcaa ttgttgatgt tagaacctac
    11101 atttgcaatt ttagacgaaa tcgattcagg tctggatatc gatgctttga aagtcgttgc
    11161 taaaggagta aatgaaatgc gcggagacaa tttcggtgca ttgatcatca cccactatca
    11221 acgattattg aactatatca cacctgatgt cgttcacatc atgatggaag gccgagtagt
    11281 catgacaggg aatgctgacc tagctaaacg tctggaagca gaaggctacg ctggtatcag
    11341 taaagaatta ggaatcgact ataaagaaga agaagcgtaa gggggagcaa gaatgaaaga
    11401 aaaaaactgg ctcgattatc ttgacgcggt aaatgatttt tctttaagta aaggcgaacc
    11461 tgattggatg agaaccttcc gtcaagacgc actagcgaaa gcagatgaac tgccattgcc
    11521 gcatatcgat cgggtgaaat tccatcgctg gtcgttattt gatgtgaaag aaacacaaac
    11581 catcagtgaa acaggcacga ttccggcatt tgatgcaatg aaagacaatc ctgtgcttgt
    11641 ccaacaaggt tcatggacga tttttgaaca actgcctgtg gaattagcag aaaaaggagt
    11701 catttttacc gacttgttca ctgcaatgat cgaatatcca gaactagtcc aagaatatta
    11761 tatgaaaaaa gcagtaaaca tgaacgaaga ccaattgact gcactccatg tagcttttat
    11821 gaacagcggt attttcctgt atgtgcctaa aaatgtcgtg atcgatgaac cactcgagtc
    11881 cttattcatt caagatggcg catccgatga gcacttcttc aagcatgtgt tgatcgtggc
    11941 agatgaacat agtgaatttt cttatctgga acgtttccag acaaccaaag aacaagtagc
    12001 aaaatcatca ggtaatatca tcgtggaagt catcgctaaa gctggttcaa aaatcaaata
    12061 ttcagccgta gatcaattag gagaaaatat tacttcttat atgaatcgcc gtgggcacat
    12121 tttacgagat gcttctgtcg actgggcaat tggtgtcatg aatgacggtc atgtgatcgc
    12181 tgactttgac tcagatttag caggagaagg tgcacacgca gaagtcaaaa tcgtggcaat
    12241 cagctcgggc agacaaatcc aaggaatcga tacacgagta accaataaag cccctcatac
    12301 gatcgggcat attttgcaac acggagtgat ccgagaaaaa ggaacattga cctttaatgg
    12361 tatcggccac atcttgaaag gggcaaaagg tgcagatgcc caacaagaaa gtcgtgtctt
    12421 gatgctttct gataaagcac gtggtgatgc gaatcctatt cttttgatcg atgaaaacga
    12481 agtaactgct ggacatgctg ccagtgtagg acgagttgat ccagaagaaa tgtactatct
    12541 gatgagtaga ggattgcata aagaagaagc agagcgctta gtgattcgag gattcctcgg
    12601 ttctgtctta acagcaatcc ctgtagaaca agttcggaaa gaattagtcg aagtaatcga
    12661 agggaagttg aatggatgat cgatgctaaa aagctacgca atgattttcc tatcttattt
    12721 caagacgtca atgatgaacc tttagtttat ctggataatg cagcgacgac ccaaaagcca
    12781 aaacaagtac tccaggcatt agcgtactat tacgaacacg ataacgccaa tgttcatcgc
    12841 ggtgtccata cgttagcaga acgcgcgacc catgattatg aacaagcaag agaaaaagta
    12901 cgggcgttca tccatgcaaa agaaacagca gaagtgctgt ttactcgtgg tacgacgaca
    12961 agcttgaatt gggtagccag aaccttcggc gaccgctata tcaaagcagg agacgagata
    13021 gtgctctctt acatggaaca tcattctaat gttatcccgt ggcagcagtt ggcaaaacga
    13081 acaggtgctg tcttgaaata tatcgaacta acagaagaag gcttcctgga tatggaggat
    13141 gcagcgaaaa agattacgga caaaaccaaa atcgtctcca ttgctcatgt atccaatgtc
    13201 ttaggtgtct tcaatcctgt tcaagaatta gctgaacttg tccatgaaaa cggcggggtc
    13261 atcgtagtag atggtgctca atctgccccg cacatggcca ttgatgtcca agcactagac
    13321 tgtgatttct ttgctttcag cggacacaaa atgtgcggac caacaggtat tggtgtttta
    13381 tatggtaaac gaaaatggtt agaagaaatg gaacctgttg aatttggtgg cgaaatgatc
    13441 gattttgtcg aattatacga cagtacatgg aaagaactac cttggaaatt cgaagcaggg
    13501 acaccaaata tcgccggcgc tatcgcatta ggccatgcaa tcgattattt gcaaaaactc
    13561 ggtatggaca atatccatcg atatgaagaa gaactagccg cttatgtgtt gccaaaatta
    13621 caagcaattg atggattgac tgtttatggt ccacaagacc cgaattttcg gacaggagtc
    13681 gttgcattca atttggatgg gttacatccc catgatgtcg cgacagcttt agacatggaa
    13741 ggggtagccg tcagagcagg tcatcattgt gcgcaaccgc tattgaagta ccttgaagta
    13801 tcagcgactg ctcgcgcgag cttttatttc tacaacacaa cgcaagacgc cgaccgttta
    13861 gttgaagcaa tcatggcaac aaaggagttt ttccaacatg ggactatcta aattagacaa
    13921 tttataccgt caagtgatct tggatcattc ttcccatccg catcatcgtg gaagcttgac
    13981 tgactcgaca agcaagatcg aattgaacaa ccccacatgt ggagatgtga tcgaattgca
    14041 agtcgatgta aaagacggaa tcatcaaaaa tatcgccttt gacggcagtg gctgctccat
    14101 cagtacggca agtgcctcga tgatgacaga cgccgtcatc ggaaaatcaa tagaagaagc
    14161 agaacattta actgtagact ttctacagct tgtccaagga aaagaagtca aagatcccga
    14221 ccaattaggc gatgctgcta tgcttggtgg tgtagctaaa tttcccgctc ggatcaaatg
    14281 tgcgacactt gcctggaaag cactaggaaa agcaatggaa aacgatggta ccgcagcttt
    14341 ccatcattac cacaaggaag aggagtgaga acatgggcgt accagaatta gaagaatata
    14401 aattcgggtt tcacgatgat gtcgaaccgg ttttcagtac aggagaaggc ttgacagaag
    14461 aagtgatccg tgagatgtca agaatcaaag gcgaaccaga atggatgctt gaattccgtc
    14521 tgaaatctct agaaacattt aacaaaatgc caatgcaaac atggggcccc gacctttcag
    14581 acatcgattt cgatgccatc aaatattacc aaaaaccaag tgacaaaccc gctcgtgact
    14641 gggaagacgt accagaaaaa atcaaagata catttgaaaa gattggtata ccagaagctg
    14701 aacgagctta tctagccggt gcttccgcac aatatgaatc agaagtcgtg taccataata
    14761 tgaaagatga attcgaaaag ttaggaatcg tctttactga cactgactct gctttgaaag
    14821 aataccctga attattcaaa gagtactttt caaaacttgt accgccaacc gacaacaaat
    14881 tagcagcgct gaattcagcc gtatggtcag gtgggacctt tatttacgtg ccaaaaggcg
    14941 tcaaagtcga tgttccatta caaacgtact tccggatcaa tgcggaaaac acggggcaat
    15001 tcgaacgtac gctgatcatt gttgacgaag gagcaagcat ccactatgtc gaaggttgta
    15061 cggcacctac ttattcaagt aacagcctgc atgccgccat cgtagaaatc tttacaagaa
    15121 aagatgcgta ttgccgttac acaacgatcc aaaactggtc agacaatgtg tacaacttag
    15181 taaccaaacg agcaaaagca tacgaaggcg caaccgttga atggatcgac ggaaacctag
    15241 gtgcaaaaac aacgatgaaa tacccaagtg tttacttaga tggaaaaggt gcacgaggca
    15301 ccatgctctc catcgccttt gcaggagcaa accaaatcca agatacgggt gcaaaaatga
    15361 tccacaatgc accaaataca tctagctcta tcgtctctaa atcaatttca aaagacggcg
    15421 gagaagtaaa ctatcgagga caagtaacct ttgccaaaaa cagtgcgggt tcgatctctc
    15481 atatcgaatg tgacacgatc atcatggacg aatggtcgaa atccgatacg atcccattca
    15541 atgagatcca caacagccaa gtctctttag aacacgaagc caaagtctca aaaatctcag
    15601 aagaacaact ctactatctg atgagccgag gcctgaccga atctgaagca actgaaatga
    15661 tcgtcatggg ctttgttgaa ccattcacca aagaacttcc aatggaatat gcagtcgaac
    15721 tcaatcgatt gatcagttat gagatggaag gcagtgttgg gtaaacctgt tataggagct
    15781 agaacccttg atatataagg gttctagctt ttttgttttg cctgtatact agaaatgacc
    15841 agattttgac cagctctttt actgatgagc catcatatca gcaaagtctt gacctgtttt
    15901 cttaggtgca ttatttgtaa tatgtgcata gatgtctaat gtcactttac tattttcatg
    15961 ccctaatcgt tgttgaacta ccttgacaga ttctcctgct tctaataata aactagcatg
    16021 tgtatgtcta aacccatgag gtgtaatacg aggcaagtta tattttttca aaatatatct
    16081 taaccaatca ttaacagtgt ttggaatgta tagagtgttt ttaactgttg taaaaacatg
    16141 ttgctcttta gagctagtat tgtatccaag ttttaaatac tcttcttttt gctgtaattt
    16201 ccagttgttg aggatagaaa gagtctcagt atctaaagag ataactcgtc tagaagctct
    16261 tgttttcgga gattgaatta taactttacc aaattcatct acagcaagag ttttattgac
    16321 atgtagttct ttattaaacg tgtctatgtc tttccactgt aaagcgagta cttggtgtta
    16381 gtatcaaatc ttagacagct tttcgtagag acaagaataa attaaactta tctacgagag
    16441 gatgaatttt tatgacccga tatgaagaaa attttaaaca aatgattgtt gaactgaatc
    16501 aaactggacg ttctgttcga gggttagcga aagaatatgg cttatctgaa gcaacgattt
    16561 acaaatggaa gaatttatat ttacctgatc agtccacagg actgactgga aaagaagtag
    16621 ctgaactgag aaaagaaaat gctcgtttaa atgaggaact tgaaatctta aaaaaagccg
    16681 cagccatatt ctctcggaaa acctaaattc gcttgttcaa tttattgaaa aatggtgtaa
    16741 ggattacact gtttctttgt tatgtcggtt attagaaatc cctagaagtg tctattattt
    16801 ttataagaat aaaccgttga cagctacaga aatcagaaat aataagttga aaaagaagat
    16861 ttcaacaatt ttttttacga ataaacaacg ctatggtgcc acaaagatcc atcaagtttt
    16921 attaaaagaa gggatttcag tatctcttaa acatgtccaa aagctaatga aacaattaaa
    16981 tttaaggtcg attgtagtta aaaaatatag acctcaaagg tctaataaac cgatcatttc
    17041 aaaagagaat ctcttaaatc aggacttttc tactgaaacg atctgtgaga aatgggcagc
    17101 tgacattacg tacattccta ctaagaaaaa tggttggtgt tacttatctt caatcatgga
    17161 tttacacacg aaaaaaatta ttagttatac attttcaaaa cgaatgactg tggattgtgt
    17221 cattcaaaca ttgaataaag caaaaataca ttatcacatt ccagaaggaa tgattctaca
    17281 cactgacttg ggcagccaat acacagcaag agaagtggaa caatggctta aaaccaacaa
    17341 aataaggcat tcttatagta gaaaaggaac accttatgat aatgctggaa tcgaatcttt
    17401 ccatgcctca ttgaaaaaag aagaagtcta cacgactagc tactcagatt ttgaagaagc
    17461 aaatcgagcg ctatttagct acattgaagg attttataac cgaaatcgaa tccatagctc
    17521 gattcactat ctaaccccac aggaatttga agaattagca aaagaaaaaa tggcttaacc
    17581 gtctctacta a
//
LOCUS       DAZKLD010000051        17345 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.051, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000051 DAZKLD010000000
VERSION     DAZKLD010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17345
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.051"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(114..659)
                     /locus_tag="ACG8KR_001409"
     CDS             complement(114..659)
                     /locus_tag="ACG8KR_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HFQ0200786.1"
                     /translation="MNALISIDYTYDFVAEDGKLTTGTAGQVIEKKLTAHTKKFIDQK
                     DFVVFAIDAHDPKDSFHPENRLFPPHNVIGTGGRELYGSLKSLYQDYEQEENVYWIDK
                     RHYSAFSGTDLDIRLRERQITDIYLTGVCTDICVLHTAVDAYNLGYKLHIFKDSVASF
                     DPVGHEWALRHFESTLGAEIL"
     gene            complement(671..1363)
                     /locus_tag="ACG8KR_001410"
     CDS             complement(671..1363)
                     /locus_tag="ACG8KR_001410"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008782 - adenosylhomocysteine
                     nucleosidase activity [Evidence IEA];
                     GO_function: GO:0008930 - methylthioadenosine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009164 - nucleoside catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HFQ0200787.1"
                     /translation="MKIGIIGAMEEEVKILRENLSEPLSWERAGALFISGSLGNHEVI
                     VVRSGIGKVLASITTSLLIQQYGVNMVINTGSAGGIGEGLQVGDIVISDKVAYFDADA
                     TGFGYNPGQLPGMPLYYEASTYLRSEMVKAAKATNLNAKEGLIVTGDTFVDSPDKIKE
                     ILTNFPEALACEMEGAAVGQTARQFNIPFLIVRAMSDTADHSATQSFDEFIEDAGKRS
                     AEMVIEFVKHLV"
     gene            complement(1382..1666)
                     /gene="macP"
                     /locus_tag="ACG8KR_001411"
     CDS             complement(1382..1666)
                     /gene="macP"
                     /locus_tag="ACG8KR_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall synthase accessory phosphoprotein
                     MacP"
                     /protein_id="HFQ0200788.1"
                     /translation="MAKGPLITRSELRKRQQAQASESLKKQRKAETAYQQEEKKIASF
                     YRKESKKNKPITKTRISEREKTTKWNSFLMKSLIIVILMLCVVFLAIAFI"
     gene            complement(1679..2242)
                     /locus_tag="ACG8KR_001412"
     CDS             complement(1679..2242)
                     /locus_tag="ACG8KR_001412"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HFQ0200789.1"
                     /translation="MLNNEQFEEKTISRKEIYSGKIIDVAVDEVRLPDGGTSKRELVF
                     HPGGVGIIAFDEQDRLLLVKQFRKPLEKVILEIPAGKIDPGEGQNPEMTAARELEEET
                     GYRAKSLSHLTSMYLSPGFANEVLHIYHAQGVEKVENPLAQDEDEVLELYHLTLEEAQ
                     QAMKDQLICDAKTIYAIQYWELLTKGK"
     gene            2470..3132
                     /locus_tag="ACG8KR_001413"
     CDS             2470..3132
                     /locus_tag="ACG8KR_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     family protein"
                     /protein_id="HFQ0200790.1"
                     /translation="MIKRNWPWLLALVLLFLFVRNNSFSFVLGLLLIGVVLVLWGLFG
                     SRRKKGKQEEIPALTDELETHYEKSGMTANEISFFRQTMNQTKNEINQLQMNMQQTAK
                     LKAIDLRHDPVKAAKALFKELVKEPNRLHEASQFLYTHLPNLVDLTNKYNEINDHEIK
                     NKQTYEKLEESAQIIDQLSALIAQDYQKFVAEDLDDIDVEISVAKQSLKRDNKYEPEQ
                     KD"
     gene            3256..4452
                     /locus_tag="ACG8KR_001414"
     CDS             3256..4452
                     /locus_tag="ACG8KR_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HFQ0200791.1"
                     /translation="MDHKPTETKDVTPVNDTLEDLLNNPFSTPTDTLTTSQQAEIDQL
                     QEQQTAARLIDKLPAERQEQAKQLAAKIDANDAQSVISYGSAAQAKLSEFSQSMLNHV
                     QAQDIGPVGDSLTELMYRLQEANPDELRAGEGNIFQRVFGKVKQSIYEVTAKYQKIGA
                     QIDKISVKLDKEKDGLLKDNLMLEQLYQKNKDYFDALNIYIAAGELKMEELQTTIIPE
                     AMKRAEETGDQMDVQIANDYTQFLDRLDKRTHDLRLARQITIQQAPQIRLIQNTNQAL
                     AEKIQASIATAIPLWKNQVVIALTLLRQKDAVTAQRQVSETTNDLLKKNSEMLKISAI
                     ETAKENERGIVDIETLQTTQNDLIETIQETLRIQKEGKEKRRHAEVELGHMEEDLKQK
                     LLDLTQ"
     gene            complement(4553..5929)
                     /locus_tag="ACG8KR_001415"
     CDS             complement(4553..5929)
                     /locus_tag="ACG8KR_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFQ0200792.1"
                     /translation="MDLFRKKEINLNHTSEMKKELKTSDLIMLGIGAIIGTGIFVVTG
                     VAANQNAGPALSLSFVLAAIVVILSGLSFAEFASRVPVIGGPYAYLYVVFGEFAAWLT
                     GWLLIGEFLLAVSSVASGWSGYMQGFLKSLGAELPQALTGGYNPENGTYIDLIAVLVV
                     IFVTYIVSLEAKKALRLNNAMVYVKFGIIALFIIVGIFFVKPDNWQPFMPFGFSGVLD
                     GAALVFFAFLGFDAVAMAAEEVKNPQKDVPRGIIGSILIATVLYIIVTLILTGIVPYT
                     ELGVNDPVAFAMRYVGHGTVGAVIAVGAILTLLTVTISMMYSLARLLFAVSKDGLLPK
                     FMTEIDQKHRTPKKATYAAGVAAVFFAGFFPLNVLAELTNIMALAYLMLVSLGLLKLR
                     KMFGKPKAGEFKVPFVPLLPIVSILTCIVLMLRLQTVTWIVFGIVMVIGLIIYFGYGY
                     GHSKMNEK"
     gene            complement(5966..6832)
                     /locus_tag="ACG8KR_001416"
     CDS             complement(5966..6832)
                     /locus_tag="ACG8KR_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GRP family sugar transporter"
                     /protein_id="HFQ0200793.1"
                     /translation="MEILIALVPMLAWGSIGLVSGKIGGDANQQTLGMTIGAFLFSLV
                     VFFVVSPVITPWIFLIGFLSGLAWSVGQNGQFHGMKYMGVSVGLPLSTGFQLILNTIA
                     GAVFFHEWTQTKDYVYGIIALALLVSGAYLTARQDDEGKIDTDNKMLDFGKGFRALIF
                     STIGYGVYTIIVNWANLDAMSIILPQSIGMILGASFFAFRKVKVDQFVWKNMICGLLW
                     GLGNICMLLTVKSLGLAVGFSLSQMGIIISTLGGIFILGERKTKKELIYVIVGCLLVI
                     LGGILLGYMKTV"
     gene            complement(6921..7946)
                     /locus_tag="ACG8KR_001417"
     CDS             complement(6921..7946)
                     /locus_tag="ACG8KR_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFQ0200794.1"
                     /translation="MNFFKYLKDQWTLILGWLFFIALTIFVMWLAPNIKVDLSTIAYL
                     ALIEGVFLIFYLLGGYLSKRRWWGKLADIQKTSPLQNYLSGGRTEEEKLVEEYINQLI
                     REHQEVMQSAISNQQDQKDYIDSWVHEIKVPLSASKLLVRSIEFDIDDQKYTLLENEL
                     SKIDEYVEQVLYYARLDSFSRDYLIQEYGLKGIIQPVIRSQANYFIQKNIRYEIVGED
                     HTVLTDAKWIAFIFNQLLSNAIKYTPDHGNIIVSIEKEAQGVSLSVKDSGIGIPAEDL
                     KRIFDKGFTGRNGRLSKTHSTGLGLYLAKNLAEKLGIHLTAESTEGKGTTMTLFFPIL
                     NFYNEKR"
     gene            complement(7964..8638)
                     /locus_tag="ACG8KR_001418"
     CDS             complement(7964..8638)
                     /locus_tag="ACG8KR_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFQ0200795.1"
                     /translation="MAKIMVVEDEEIIRQLIMEELEKWQFETFGTTDFNQVFSDFERE
                     EPQLVLLDINLPVLDGYYWCQKIREVSKVPIIFISSRNTNMDMIMAMNMGADDFVTKH
                     FQIDVLIAKINALLRRSYNYTELSSEMMSHNGITLNVDNGSMEINGEIIDLSKNEYRL
                     LFILMKHNGKILSREKLLRALWDDERFVDDNTLTVNINRLRRKIEQAGIQGYIETKVG
                     QGYIVP"
     gene            complement(8822..9715)
                     /locus_tag="ACG8KR_001419"
     CDS             complement(8822..9715)
                     /locus_tag="ACG8KR_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFQ0200796.1"
                     /translation="MKKKIGNRPQTQISLLLQLFMAVMFLAGAAVFTYPFLADALSNY
                     LDQRRIENYQKQLERDKEEKHEQQLAVHEKKNQTLAHTSVIPGMGQVKDPFEQAVRNV
                     QNPGKEYYEQHMIGAIYIPKINVSLPLFDETNDLLLDRGATVLQGTSFPTGGESTHSV
                     ITAHSGIAEKKLFTDLEEMEQDDRFYLEVYGRMLAYEVVEKIVVLPTKTNTLAIREKQ
                     DLVTLITCTPYTVNTHRLLVTGRRVPFTEEVSSKIEQTKKYHLYRLLILLLGILLVLT
                     LFGYWCYRKFKRQKQRKKNNR"
     gene            complement(9789..11486)
                     /locus_tag="ACG8KR_001420"
     CDS             complement(9789..11486)
                     /locus_tag="ACG8KR_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFQ0200797.1"
                     /translation="MNKKMIVTGMSVLLLVTAGIGIFGNGKLVKAEEVAQKPSEVTIT
                     LHKKGFSSVPEERPNSGLVSTDFGEENIPGVDFDLFDVTEVYYDLIRDNPLTPEREDG
                     LNSAEAIEWIQKRHTESWFLKYRLTSIDKQTTNEAGEAVFSTVQVTEEAPSSRDKVYL
                     FLETYSPAHISRIASPMVVMMPVMMPDMVDGVWDGSTWKDTYNTDVHLYPKNEIREAD
                     KQMNVEESDLRQVTIINEAGEQETISYIDLERGKTASYTITAPIPYFIDSVLENGSAV
                     IKNYKITDTPTVGLTYYDQEIEVRAGETILTKGQDYIVEVVSNGFVVTILTEENGVAK
                     VDTLGRLADARGGDLTITYNLKVSTELEADDFHNNTAVIEIGRNDEFDYEEGVEPPEK
                     VTTGGRKFEKYDASSSELLKDARFELWNEDRSEYAIFYKGESPLAVYESGADRIEWAT
                     SGQATEFVADGNGYFEVQGLDYGTYQMKETMAPEGYVLPTGEAAFTEFIISYGSYNEE
                     IQIVGVENPGPERVPNMKRGSLPATGGNGLLAFLLIGISLMIGAYSWYRKSKMKSEV"
     gene            complement(11479..12792)
                     /locus_tag="ACG8KR_001421"
     CDS             complement(11479..12792)
                     /locus_tag="ACG8KR_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin N-terminal domain-containing protein"
                     /protein_id="HFQ0200798.1"
                     /translation="MKKLLCLMLQLLVLLPLAAVLLPHAMSAEESGEDVVEFVLHKRM
                     IRDIDYNDQFEYHENDGLAISEEAGETADIISQTVPLNGATFAIYDMTDYYHEKKAAE
                     NMTPKEFVQQIAETNRNNIRQLITEERLVQIGTSVTTGKDTVGGLGDGIARIKAPKKN
                     QDRDAVYLIFEESIDPEVGLDVDIDQTAIPIAAILPIYHPTESTEELQEIHIYPKNVG
                     YLRDPYFFKYGRQRGTTEKGKPLEGAVFALYQMIEGGKYYLDMAPANDLKNQWIKPAD
                     NDPLNDKNVSKFISDKEGLVTTGQRFLPSGTYYFEELKGVEGYEMDEASKRIEVIIPE
                     FWVDAKGQPQYVVVNGQEMAELESGKVPVSAYESATPRVYNETVKETTDKPKEPSGPS
                     NPGQSGKWSVFPQTNEARTLISLLGILLLLGAVLLIKKERGEKNE"
     gene            complement(12792..16733)
                     /locus_tag="ACG8KR_001422"
     CDS             complement(12792..16733)
                     /locus_tag="ACG8KR_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFQ0200799.1"
                     /translation="MEKLDEPIVPSGQALQRIQRQLFNGILERLGPTATQDPYEYIYP
                     SGEVENEQHRYPKFTTNDYTKVLGNWTQSNERYQGDKGSPYDGENLIDDSDYTNGGAN
                     WRNYDYATNNGSTAGEEDRNVPAQTVQLWGNERNFENSYLDYNGAYIKKWVEPVLPSS
                     TASDLHPEDATTLYNVYLDVIGGEKKEISPIDIVFVLDKSASMSELTAGTNSQTKNAA
                     LIEAVNEMSKDLLSDPSLDIRIGMVNFYHNSTAINNHEQISSDIFPLTNDINRLTGSE
                     NTALNRTPIGGTPLTLGLKNGYETLYKDNGGENRNPEKILIVVGDGTPTFSYAPIQSS
                     YRTSTNGAWSNWTVMEDKIAEDNDVLFRNFEEFSGNTSNAGFTHPVTYASDFNRPEDE
                     VNVHYRYGEVKEGDNKATHWVGDGSSNNNTNGSPTSQEKSSAINTVAYHHWLKNKYQE
                     NPPSIFSIGLGIDGNVSGRQRLDAIGRNVLKNIADLEEDGVTPRYYNANNKNDIVTAL
                     EDISSTFKRTIQQATLYDETGFNVSPFGAGRSADIQFYHLDPGENGMKYQEPEIWDEE
                     RHGQAPPEVVATPTTYVGRENQAYKFSPISLGEGEMVRIKYQVKLDAGAQDGNFCTVN
                     NMAYIQNLEDYENDITTGRMYFPSSSIRYEHKGRNFQVSKKGQNGEPLAGVEFALYDQ
                     NPESGEAEPIETSKTTTDGIAIFETKMPILGDETGAVSDIFWIREIAGPPRYQLVDET
                     YQFQIKKYAANEDEMLGHYELVDEYTSGSRVGGPEGKDPNSYYEGEYHKLSVVQEPNI
                     EGTEYDELYVKLEMRNEFKPIHMNINKLIEGSNMPINGAEFELYETDGNGLEIGYPVA
                     KGLSGVNEVEEEKGRLTFYSVDEDGKYVIVDGEKVIHPIGEPDSFVYKDSEETTNYAE
                     YKIIESQNPEGFKEPDENDTWILRLYDNAIGTIQLKQTGEENWQYLTYTTDEEDRLWV
                     AFTAYNTFDYREIRVRKLDHEGHTLDGAGFDIRKVGDSSFPLYRAYTGNPLEPEESKR
                     LPGIGYFYSRAVGVEKYVDLNYDQPLHLSIGEYEVSESVAPNGYRLSDQVFQFALNEE
                     GKFIIEDTVIEDETAPLPEGYEIIDGVLQVTLEDELAPIDLELLKIDSMNSRRLEGAE
                     FSIEKQNSAGEYEIIEGGLAPDPNDSSLFNASDLLEGVYRIKEVKSPDSYRKLPGYFI
                     LEISYREEPDIENDRVVPGKEAGTLKVEISYYPNEEAETPDLQQELEYELLETNRIRL
                     QLSVGNDPENPLPATGGSGRLFYILLAAFFMALAGAAYGLFVRQSKKEGAR"
     gene            complement(16842..17300)
                     /locus_tag="ACG8KR_001423"
     CDS             complement(16842..17300)
                     /locus_tag="ACG8KR_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFQ0200800.1"
                     /translation="MKGLKLKLLQLVTVSLLFGPYFQSVYHMTTLDVAAESTETKEDE
                     VFLDEDYAKIEGKYIEKEETLEWHLAFEKKESANEGRIRLAIDAAAAGIGTVKNVRGT
                     GLSSYDEDGEDLKVETIDGQEWYVGKVYSKEAETGTLIFETEKIPDVNEG"
BASE COUNT         4860 a         3893 c         3019 g         5573 t
ORIGIN      
        1 agaataagtt tttttaaaat aacgtataaa aaagagcata aaacaaagtc gtttgtttta
       61 tgctcttctt ttattatttt gtgcttttta ttttatcaaa tcactttgtt tttttataaa
      121 atttctgcgc ctagtgtgga ctcaaaatgt ctcaatgccc attcatgtcc aacgggatcg
      181 aaacttgcca cggaatcttt gaaaatatgg agtttgtatc ctagattata cgcatcgact
      241 gctgtatgca acacacagat atctgtacat acgccggtca aataaatatc tgtgatctgg
      301 cgttctcgca aacggatgtc tagatctgtt ccactaaaag cggaataatg ccgtttgtct
      361 atccaataaa cattttcctc ttgctcatag tcttggtata aactttttaa agagccgtac
      421 aattcccgtc cacccgttcc tatcacatta tgcggaggaa ataagcggtt ctccggatga
      481 aaggaatctt ttggatcatg cgcatcaatg gcaaaaacga caaaatcctt ttgatcaata
      541 aacttctttg tatgagcagt cagctttttt tcaattactt gtcctgctgt cccagtagtc
      601 agctttccat cctctgcaac aaaatcatat gtgtagtcaa tcgagatcaa tgcgttcatc
      661 ttagtcctcc ttatacgaga tgtttgacaa attcgatcac catttctgct gatcgtttgc
      721 ctgcgtcttc gataaattcg tcaaagcttt gtgtcgccga gtgatctgct gtgtcgctca
      781 ttgcacgtac gatcaaaaat gggatattga actgacgagc tgtttgtcca acagcggctc
      841 cttccatttc gcaagctaat gcttcaggga aattcgttaa gatttctttg attttatctg
      901 gcgagtcgac aaatgtatca cctgtaacga tcaatccttc ttttgcgttc aaattcgttg
      961 cttttgctgc tttaaccatt tcactgcgca aatatgtgct tgcttcataa tatagaggca
     1021 ttcctggtaa ttgtcctggg ttgtagccaa atcctgttgc atctgcatca aaataagcta
     1081 ctttgtctga tataacgatg tcacctactt gtaagccttc accgattccg ccagctgaac
     1141 ctgtattgat caccatgttc acgccgtatt gttggatcaa cagacttgtg gtaatagatg
     1201 ctaagacttt tcctataccg gaacgtacga cgatcacttc gtggttacct aatgaacctg
     1261 aaataaataa agcgcctgcg cgttcccatg ataatggttc gcttaggttc tctctcaaaa
     1321 tttttacttc ttcttccatt gcgccgataa taccgatttt catcttttta attccttctt
     1381 tctatataaa tgcgattgct aaaaaaacca cacataacat taggataaca ataataagag
     1441 atttcatcaa aaaagagttc catttggtcg tcttttcccg ttcgctgatc cttgttttcg
     1501 tgattggttt attctttttg ctttctttac gataaaagct ggcaatcttt ttttcttctt
     1561 gctgataagc tgtttctgct tttcgctgtt tttttaatga ctcgcttgct tgtgcttgtt
     1621 gtcgcttgcg aagttcgctt cgcgtaataa gcggtccttt ggccataagc cgatccctct
     1681 actttccttt ggtcaatagt tcccagtatt gaatcgcata aatcgtcttt gcatcacaaa
     1741 tcaactgatc tttcatcgct tgttgcgcct cttcaagagt tagatgatac aactccagta
     1801 cttcgtcttc atcttgtgct aaaggattct cgaccttctc taccccttgt gcatgataaa
     1861 tatgaaggac ttcatttgca aaacctggtg aaagatacat cgaagtaaga tggctcagcg
     1921 acttcgcacg atagccagtt tcttcttcta attctctagc tgctgtcatt tcgggatttt
     1981 gtccttctcc tggatcgatt tttccagctg gaatctccaa aatcactttt tctaaaggtt
     2041 ttcgaaattg tttgacaaga agcagacgat cttgttcgtc aaaagcaatg atcccgacac
     2101 cgccaggatg aaataccagt tctctcttcg aagttcctcc atctggcaaa cgaacttcgt
     2161 ctacagcaac atcaataatt tttccgctat agatttcttt gcgactgatt gttttttctt
     2221 caaattgctc attgttcagc atatcttctc ctgctttctt tgtaatcata actgcttttt
     2281 tttcattttt caatttttct caaaaactcc ttattagttt accatagata aaccttagat
     2341 tttctctaaa tatagaaaat ccttctttag tccgatactc tttctagtag tccgtatgct
     2401 aaaataagta tgtaatcgaa taacgaaaag aattcaatac tcaacgataa atgacatgga
     2461 gggtttatta tgataaaacg aaattggccg tggcttctag cattggtctt gctatttctc
     2521 tttgtccgaa acaatagttt ttcttttgtt ctaggacttt tattgatagg tgtagtttta
     2581 gttttgtggg gactctttgg ttcgcgcagg aaaaagggaa aacaagaaga aattcctgca
     2641 ctgactgacg aacttgaaac tcactatgaa aaaagcggta tgacagcgaa tgaaatttca
     2701 ttttttcgac aaacaatgaa tcaaacaaaa aatgagatca atcaattgca aatgaatatg
     2761 cagcagacag ccaaacttaa agcaatcgat ctccgccatg atccagtcaa agcagcgaaa
     2821 gctttattca aagaattggt gaaagaacca aaccgactgc atgaagcaag tcagtttctt
     2881 tatacgcatc tgccgaatct agttgatttg acaaataagt ataatgagat caatgaccat
     2941 gagatcaaaa acaaacagac ctatgaaaaa ctagaagaaa gcgcgcagat catcgaccag
     3001 ctgtcagctt taatcgcaca agattatcaa aaattcgttg ctgaggatct agatgatatc
     3061 gacgtagaga tatctgttgc gaaacagagc ttaaagcgtg ataataaata tgaaccagaa
     3121 caaaaggatt aactcttttg ttcataaaag aagactcgga acattcagat gatgtctggc
     3181 tgtttcgagt actttttcta aagtagagac attcatgtaa gaatcgagaa cagattcaac
     3241 aaggaggaat tttttatgga tcataaaccg actgaaacaa aagatgttac accagttaac
     3301 gatacacttg aagatttatt gaacaacccg ttttcaactc caacagatac gctgaccact
     3361 tcacaacaag cggagattga tcagctccaa gagcaacaga cagctgctcg tttaattgac
     3421 aaactaccgg cggaacgcca agaacaagct aaacagctag cagcaaaaat cgatgctaat
     3481 gatgcacagt ctgtcatcag ctacggatcg gctgcccaag caaaattaag tgaattctca
     3541 caatcgatgt tgaatcatgt gcaagcacaa gatattggtc ctgtaggaga ttccttaact
     3601 gaactgatgt atcgtctcca agaagccaat cctgacgaac tgcgtgccgg agaagggaac
     3661 atcttccagc gtgtcttcgg gaaggtcaaa caatcgatct atgaagtgac agcaaaatat
     3721 caaaagattg gtgcacaaat tgacaagatt tcagtgaaat tagataaaga aaaagacggt
     3781 ctgctaaaag ataatctgat gttagaacag ctttaccaga aaaacaaaga ctactttgat
     3841 gcactgaata tttatatcgc tgctggagaa ttgaaaatgg aagaacttca aacaacgatc
     3901 attccagaag cgatgaaaag agcagaagaa actggcgatc agatggatgt tcagattgcc
     3961 aatgattaca cgcaattcct agatcgtctg gataaacgta cgcatgatct tcgcttagct
     4021 agacagatca cgatccagca agcaccacaa atccgtttga tccaaaacac aaatcaggcc
     4081 ttggctgaaa agatccaagc atctatcgct acagccattc cgctgtggaa aaaccaagtc
     4141 gtgatcgcat tgaccttgct tcgtcaaaaa gatgcagtaa ctgctcaacg ccaagtctct
     4201 gaaacaacaa acgacttatt gaagaaaaat tcagaaatgc tgaaaatctc agccatcgaa
     4261 acagcgaaag aaaatgaacg tggaatcgtg gatatcgaga cccttcaaac cactcaaaat
     4321 gatttgatcg aaacaatcca agagacattg cgtatccaaa aagaaggaaa agaaaaacgt
     4381 cgtcatgcag aagtagagct tggacacatg gaagaagatc tgaaacaaaa actgttggat
     4441 ttaacccaat agatcacctt tcgcttgtta ctaaaacaaa cgcccaacaa cagtttatta
     4501 caactgttga tgggcgtttt tcttatcttt cttctaactt ctccattgga gtctatttct
     4561 cattcatctt gctgtgtcca tagccatagc caaaataaat gatcagtcca ataaccatta
     4621 cgataccgaa cacgatccat gtcacagttt gcagacgaag catcaataca atacaagtca
     4681 aaatggagac gattggcaat agcggaacga atggcacttt gaattctcca gcttttggtt
     4741 tgccaaacat ttttctcaac ttcagtaagc ctagactaac aagcatcaaa taggcgagcg
     4801 ccatgatatt cgttagttcc gctaatacat tcaatgggaa aaatccggca aagaatacag
     4861 ctgccacacc agcggcataa gtcgcttttt tcggtgtacg atgcttttga tcgatttctg
     4921 tcataaactt aggaagtaag ccatccttgc tgacagcaaa cagtaaacga gcaagtgaat
     4981 acatcatcga gatcgttacc gtcagtagcg ttaaaattgc ccctacagcg atcaccgcgc
     5041 ccactgttcc atgtcctaca tacctcattg caaaagccac tgggtcattg actcctagtt
     5101 cagtatacgg aacgattcct gtcaagatca gcgtcacgat gatgtaaagg acagtagcaa
     5161 tcagtatcga accaatgatt cctcttggca catctttttg cgggtttttg acttcctcgg
     5221 ctgccattgc aacggcatca aatcccaaga aagcaaaaaa cacaagagct gcaccatcta
     5281 gcacaccgga aaaaccaaaa ggcatgaatg gctgccagtt atcaggttta acgaaaaata
     5341 ttcctacaat gataaacaac gcgatgatac caaatttgac atataccatg gcattattca
     5401 aacgtaatgc tttcttggct tcaagcgaca cgatgtaagt gacgaatatc actacgagca
     5461 ccgcaatcaa gtcaatgtat gtaccatttt ccggattgta cccgccagtt aatgcttgtg
     5521 gaagttcggc ccctaaactt ttcaaaaaac cttgcatata gccagaccat ccggaagcaa
     5581 cagaggaaac cgctagtaaa aattccccga tcaacaacca ccctgtcagc caggcagcaa
     5641 attcaccaaa tacaacatac agataggcat atggcccacc aatcactggc actcgcgaag
     5701 caaattctgc gaaacttagt ccagacaaaa tgacaacgat tgcagctaag acaaatgaaa
     5761 gtgacagtgc tggaccagca ttttgattgg ccgcaacccc tgtcaccaca aatattcccg
     5821 taccgatgat cgcaccaatt ccaagcatga tcaaatcaga cgtttttagt tcttttttca
     5881 tctcactcgt atgattgaga ttgatctctt tcttacgaaa caaatccatt gaagttcctc
     5941 cccattttag gttggaaaac agcgattaaa ctgttttcat ataccctaaa aggataccac
     6001 ctaaaatgac taaaaggcaa ccaacaataa cataaatgag ctcttttttc gtttttcttt
     6061 cgccaaggat gaaaatccca cctaaagtag agataatgat ccccatttgt gaaagtgaga
     6121 agccaacagc taaaccaagt gatttgactg ttagaagcat gcagatgttt cctaagcccc
     6181 ataataatcc acagatcatg tttttccaca caaactgatc gactttaact ttacggaaag
     6241 caaagaagct tgcgcctaag atcattccga tactttgagg taagataatt gacatcgcat
     6301 ccaaattcgc ccagttgacg atgatggtat atacaccata accaatcgta gagaagatca
     6361 atgctctaaa acccttacca aaatcaagca ttttattatc tgtatctatt tttccttcgt
     6421 catcttgtcg ggcagtcaaa tacgcaccgc ttacaagaag agctaaagca ataataccgt
     6481 atacataatc tttcgtttgt gtccattcat ggaagaaaac ggctcccgca atcgtattca
     6541 aaattaattg aaaaccagta gacaatggta gccctacaga aacacccatg tacttcattc
     6601 catggaactg tccattttgt ccaacactcc aagctaagcc ggataaaaat ccgatcaaga
     6661 aaatccaagg ggtgattaca ggactaacaa cgaaaaagac aactaaagaa aacagaaatg
     6721 caccgatcgt cattcctagt gtttgttgat tggcatcgcc accaatttta ccacttacta
     6781 aaccaatact accccatgcc aacataggga ctaatgctat taaaatttcc ataaccaatc
     6841 tccttttatt tcacatgata agaaacttat cagcgaaaac gttaacaaca caagcacttt
     6901 ttttcgtttc taacgatttt ctaacgtttt tcattataaa aatttaagat tgggaaaaat
     6961 aaagtcattg ttgtcccttt tccttcagtt gattcagccg tcaaatggat acctaatttt
     7021 tcggccagat tttttgctaa ataaagtcct aatcctgttg aatgtgtttt gctaagtcga
     7081 ccatttctcc cagtaaatcc cttatcaaag atccgtttca agtcttccgc tgggataccg
     7141 atccctgaat ctttaactga aaggctgact ccttgcgctt ccttttctat gctcacaatg
     7201 atgtttccgt gatccggcgt atatttgatg gcattactaa gcaattggtt gaagataaaa
     7261 gcgatccatt tagcatcggt caaaacagta tgatcctcac caacgatctc gtagcgaata
     7321 tttttttgga taaaataatt tgcctgactg cgtatcacag gctgaatgat tccttttagt
     7381 ccatattctt ggatgaggta gtcccgcgag aaactgtcga gtcttgcata atacaaaact
     7441 tgttctacgt attcatcgat ttttgataat tcattttcta gtaatgtata cttttgatcg
     7501 tcaatatcga actcgatcga acgaactagc aatttagaag cggaaagtgg cactttgatt
     7561 tcatggaccc atgagtcaat ataatctttt tggtcttgtt gattactgat tgctgattgc
     7621 attacttctt ggtgttcacg gatcagctga ttgatatatt cttctaccag tttttcttct
     7681 tctgtcctcc caccactcaa ataattttgc aaaggggaag ttttttgtat gtcggctaat
     7741 tttccccacc atcttctctt gctcaagtaa ccacccagca gataaaaaat cagaaagacg
     7801 ccctcaatca aagctaaata agcaattgtg ctcaaatcaa ctttgatgtt tggcgccagc
     7861 cacatcacaa agatcgtcaa tgcaataaaa aacagccatc ccaagatcaa tgtccactga
     7921 tctttcaaat atttaaaaaa attcatctta accgctcatc ctttcaggga acgatatatc
     7981 cctgtcctac ttttgtttcg atatatcctt ggatacctgc ctgttcgatt tttcggcgta
     8041 accgattgat gttgactgtc aatgtattgt catctacaaa tcgttcgtca tcccacagtg
     8101 ctcgcagcaa tttttcccga cttaagatct ttccattgtg cttcatcaaa ataaataata
     8161 gacgatattc atttttgctc aaatcaatga tctctccgtt gatttccata cttccgttgt
     8221 caacattcaa cgtaatgcca ttgtggctca tcatttcact agataattct gtgtagttgt
     8281 aagaacggcg aagtaatgca ttgatttttg caatcagtac atcgatttgg aaatgttttg
     8341 tcacaaaatc atctgccccc atgttcatag ccatgatcat atccatattc gtattcctgc
     8401 tggagataaa aatgatcggt actttcgata cttcacgtat tttttgacac caataatagc
     8461 catccaaaac gggaagatta atatctagta atacaagctg tggttcttct ctttcaaagt
     8521 ctgagaacac ttgattgaaa tcggtcgttc caaaggtttc aaattgccat ttctcaagtt
     8581 cctccatgat caattgtcga atgatctctt cgtcttcgac taccatgatt tttgccataa
     8641 aaaatccctc catctttctt cttgctacta caatatcaaa gaagcccttg tgaaacaagt
     8701 tggcttacct ggaaaatgtc attttagcaa acgttaaaaa aaactggaaa tatgcgattt
     8761 aatcgcgttt gtctcttcca agcagcttaa atttattatg ttattcttaa taattggtct
     8821 attatctgtt atttttcttt cgttgttttt gtcttttaaa ttttcgataa caccaataac
     8881 cgaaaagcgt tagcactagc aatataccta gaaggagtat aagtaatcga taaagatggt
     8941 atttttttgt ctgttctatc tttgaagaga cttcttctgt aaagggaact cgtcttcctg
     9001 tcactagtaa tcggtgagtg ttcacggtgt aaggtgtaca agtgataagt gtgactagat
     9061 cctgcttttc tctaattgct aatgtatttg tttttgttgg aaggacaaca attttctcaa
     9121 cgacttcata agcgagcatc cgaccataca cttcaagata gaatcggtca tcttgctcca
     9181 tttcttcaag atccgtaaag agttttttct cagcaatacc gctatgggcg gtaatcacgg
     9241 aatgtgtact ctctcctcca gttggaaaag aggtgccttg caggactgtc gctcctctat
     9301 ctaacaaaag atcattcgtt tcatcaaata aaggcaagct aacattgatt ttcggaatat
     9361 agatggctcc gatcatatgt tgttcgtaat attcttttcc tggattttgg acatttctaa
     9421 ccgcttgctc aaaaggatct ttgacttgtc ccattcctgg gattactgat gtatgagcaa
     9481 gcgtctgatt tttcttttca tgcactgcta attgttgctc atgtttttct tctttatcgc
     9541 gttcaagctg cttttgataa ttctcgatcc ttctttgatc caaataattg ctgagagcat
     9601 ctgctaaaaa tggatatgta aaaactgcag ctccggcaag aaacatcact gccataaata
     9661 attgtaataa aagactgatt tgggtttgag gtctattacc tattttcttt ttcattttct
     9721 tcaccttctt atttaaaaaa actgcagcaa cctttataaa accgattgct gcagcttatt
     9781 tttgttgctt atacttcaga cttcatttta gatttacggt accagctgta cgctccaatc
     9841 atcaaactta ttccgattag taagaatgcc aagagcccgt tcccaccagt agcaggaagt
     9901 gacccgcgtt tcatattcgg tactctttct ggccctggat tttctactcc tacgatctga
     9961 atttcttcat tatagcttcc ataagagatg ataaattcag taaaggctgc ctcacctgtt
    10021 ggaagtacat aaccttctgg cgccatggtc tctttcattt gataggttcc ataatctaac
    10081 ccttgaacct caaagtatcc atttccatct gcgacaaatt ctgttgcttg tccgcttgtt
    10141 gcccactcaa ttctatctgc tcctgattca taaacagcta aaggcgactc ccctttataa
    10201 aatattgcgt attcagaacg gtcttcattc cacaattcga atcttgcatc tttaagcaat
    10261 tcactgcttg atgcatcata tttttcaaac tttcttccac ctgttgtgac tttctctggc
    10321 ggctctactc cttcttcata atcaaattca tcatttcgtc cgatttcaat aaccgctgtg
    10381 ttgttatgga aatcatccgc ttctagttct gtggaaacct tcaaattata cgtgatcgtt
    10441 aaatctcctc cacgtgcatc tgctaatcta cctaaagtat ctactttggc tactccattt
    10501 tcttctgtca aaatcgtcac gacaaacccg ttgcttacta cttctactat ataatcttgt
    10561 ccttttgtta aaatcgtctc gccggctcgc acttctattt cctgatcata ataagttaga
    10621 ccaactgtag gcgtatctgt gattttgtaa tttttgataa cagcgcttcc gttttcaagg
    10681 acagaatcga tgaaataagg aataggtgct gtgatcgtgt aagaagccgt ttttcctctt
    10741 tctagatcaa tataagaaat cgtttcttgt tctccagctt cattaataat cgttacttgt
    10801 ctaaggtcgc tttcctctac attcatctgt ttatctgctt ctctgatttc gtttttcgga
    10861 tacaaatgca catctgtatt gtatgtgtct ttccatgtag aaccatccca cacgccatcc
    10921 accatatcag gcatcatcac tggcatcata acaaccattg gagacgcgat tctggaaata
    10981 tgagctggcg aataagtttc taagaaaaga tagactttat ctctagaaga aggtgcttct
    11041 tccgtcactt ggacggtaga gaataccgct tctccagctt cattcgttgt ttgtttgtca
    11101 atacttgtca gtcgatattt taaaaaccaa ctttctgtgt gccgtttttg aatccattca
    11161 atcgcttcgg cagaattaag accgtcttct cgttccggtg ttaaaggatt atctcgaatc
    11221 aaatcgtaat atacttccgt tacgtcaaac aaatcaaaat cgactcctgg aatattttct
    11281 tcaccaaaat ccgtagaaac caatccgcta ttcggtcgtt cttccggaac agatgagaaa
    11341 ccttttttgt gtaatgtaat cgtcacttca cttggttttt gtgcgacttc ttcggctttg
    11401 actagttttc cgttcccgaa aatccctatt cccgcagtaa cgagtaacag tacactcatt
    11461 cccgtgacaa tcattttttt attcattttt ttctccccgt tcttttttta ttaataaaac
    11521 agcacctaac aataaaagaa ttcctagtaa agaaatcaac gttcttgctt cattcgtttg
    11581 cgggaatacg gaccattttc ctgattgacc tggattgctt ggaccactcg gctctttcgg
    11641 tttgtccgtt gtttctttta cggtctcgtt ataaacacgt ggtgttgcgg attcataggc
    11701 agagactgga actttcccgg attctaattc agccatctcc tgtccgttaa ctactacata
    11761 ttgtggttgg ccttttgcat ccacccaaaa ttcagggatg atgacctcga ttctcttact
    11821 tgcttcatcc atctcgtaac cttccacacc ttttaattct tcaaaataat aggtgccaga
    11881 aggcaagaaa cgttgtcctg tcgtaacaag gccctcttta tccgaaatga atttgcttac
    11941 atttttgtca ttcaaaggat cattgtctgc tggtttgatc cattgatttt tcaagtcatt
    12001 agcaggagcc atatctaaat aatatttccc cccttctatc atctgatata gagcaaaaac
    12061 agcaccttct aatggttttc ctttttcagt tgttcctctt tgacgtccat acttgaagaa
    12121 gtatggatct cttagataac cgacattttt aggatagata tgaatctctt gcagttcctc
    12181 ggttgattct gtcgggtgat aaattggcaa aatagcagca attgggatag ctgtctgatc
    12241 gatatcaaca tccaatccaa cttctggatc aatcgattct tcgaaaatca aataaactgc
    12301 atcccgatct tggtttttct taggtgcttt gatacgtgca atcccgtctc ctaatccgcc
    12361 aaccgtatct ttcccagttg ttaccgaagt ccctatttgg acaagccttt cttctgtgat
    12421 tagctgacgg atattgtttc tgttcgtttc agcgatttgc tggacgaatt ctttaggagt
    12481 catgttttca gcagcttttt tttcatgata ataatcggtc atgtcgtaaa tggcaaatgt
    12541 cgctccattt agtggcaccg tctgtgaaat gatatctgct gtttcccctg cttcctcaga
    12601 gatagccaag ccatcatttt catggtattc aaattgatcg ttataatcga tatctcgaat
    12661 catccgttta tgcaacacga attccacgac atcttcccct gattcttctg ccgacatcgc
    12721 atgtggaaga aggactgctg caagcggtag taaaacaagt aattgaagca tcaaacacaa
    12781 cagctttttc attaccttgc tccctccttt ttagattgtc tgacaaacaa accataggct
    12841 gctcctgcaa gcgccatgaa gaatgcagca agcaggatat agaataatcg tccggatcct
    12901 cctgtagcag gcaacggatt ttcaggatcg ttcccgacac taagctgtaa tctgattcga
    12961 tttgtctcca aaagttcata ttctaactct tgctgtagat caggcgtctc tgcttcttcg
    13021 tttggataat acgaaatctc aacttttagt gtacccgctt cttttcctgg aactacccga
    13081 tcattttcta tgtcaggttc ttctcgataa ctaatttcta aaataaagta acctggaagt
    13141 tttctatagc tgtcaggtga cttcacttcc ttgatccgat agaccccttc caacaagtct
    13201 gaagcattga atagggaaga atcgtttgga tctggtgcca agcctccctc gatgatttca
    13261 tactctcctg cactattttg tttctcaatt gaaaattctg cgccttcaag acgtcggcta
    13321 ttcatactgt caattttcaa aagttccaaa tcgattggcg ctagttcatc ttctaaggtt
    13381 acttgtagta caccatcgat gatttcatag ccttccggca agggagcagt ttcgtcttcg
    13441 atgaccgtat cttcaatgat aaacttacct tcttcattca gtgcaaactg gaacacttga
    13501 tcagatagcc gatacccatt tggtgcgaca gattccgaga cctcatattc accaatagac
    13561 agatgcagag gttggtcgta atttaaatcg acatactttt ctactcctac ggcacgagaa
    13621 tagaagtatc cgatcccagg caaacgtttc gattcttctg gttctaacgg attaccagtg
    13681 tatgctcgat acagcgggaa ggaagaatcc cccactttcc tgatatcgaa acctgcacca
    13741 tctaatgtat ggccttcatg gtctaatttt ctaactcgga tctcccgata atcaaacgta
    13801 ttgtaagcag tgaatgctac ccataaacga tcctcttcat ctgttgtata cgtgagatat
    13861 tgccaatttt cttcacctgt ttgcttgagt tggatagtcc ctatagcatt atcgtaaagt
    13921 cgcaagatcc atgtgtcatt ttcatcaggt tctttgaaac cttctgggtt ctggctctca
    13981 atgattttgt attctgcata gttggtagtt tcttcactat ccttataaac aaatgaatct
    14041 ggctctccga ttggatgaat aactttctca ccatccacga ttacatattt cccgtcttca
    14101 tctactgaat agaacgtgag tctccctttc tcttcctcta cttcgttcac accagaaagc
    14161 cctttcgcta ctggatatcc gatttccagt ccgttaccat ctgtttcata aagttcaaac
    14221 tctgcgccat tgattggcat attactgcct tcaattagtt tattgatatt catgtggatt
    14281 ggcttgaatt catttcgcat ttccagtttg acatacaact catcatactc tgtgccttct
    14341 atgttaggct cttgtacaac agaaagcttg tgatattctc cttcataata ggaattcgga
    14401 tctttacctt ccggtccgcc gactctcgaa ccagatgtat attcatctac aagctcgtag
    14461 tgacctaaca tctcatcttc gttggctgca tattttttga tttggaattg atacgtctca
    14521 tctactaact gataacgtgg tggtccagcg atctctctga tccaaaatat gtcgctgact
    14581 gctccagttt catctcctaa aatcggcatc ttcgtttcaa agatcgctat tccgtccgtg
    14641 gtagttttac ttgtttcgat tggttcggct tccccactct ctggattttg gtcatataat
    14701 gcaaattcga ctccagccaa cggctcgcca ttttgaccct tcttgctgac ttggaaatta
    14761 cggcctttat gttcgtaacg aatggaagaa gatgggaaat acatccgtcc agtagtgata
    14821 tcattctcat aatcctccag gttctgaata taggccatat tgttcactgt gcaaaaattt
    14881 ccgtcttgag ctcccgcatc aagttttact tggtacttga tcctgaccat ttccccttca
    14941 cctaaagaaa tcggagaaaa tttataagct tggttttccc gtccgacata cgttgtcgga
    15001 gtcgccacta cttctggtgg ggcttgtcca tgacgttctt catcccatat ttctggctct
    15061 tggtacttca tgccgttttc tcctggatct agatggtaaa actgaatatc tgccgatctg
    15121 ccagctccaa atggtgagac attaaaacca gtttcatcat aaagtgtggc ttgctggatc
    15181 gttctcttga aggtagaaga gatatcctcc aaggctgtaa caatgtcatt cttgttgttc
    15241 gcattgtaat atcgcggtgt tacgccatct tcttctaaat cagcaatatt tttcaataca
    15301 tttcgtccaa tagcatctag tcgttgtctc ccgctaacgt ttccatcgat tcctaaacca
    15361 atggagaaga tacttggcgg gttctcttga tacttgtttt tcagccaatg atgataagca
    15421 actgtgttga tcgcagatga cttttcttgc gaagtaggac tgccattcgt attgttatta
    15481 gaagaaccat caccaaccca gtgagtcgct ttattatctc cttcttttac ctctccataa
    15541 cgatagtgta catttacttc atcttctgga cggttaaagt cagatgcata ggtaactgga
    15601 tgagtaaacc ctgcattaga agtattccca gaaaactcct caaaatttct gaacaaaacg
    15661 tcattatctt cagcaatctt atcttccatc acagtccagt tgctccaagc tccattagtg
    15721 ctcgtacgat aacttgactg aatcggtgca taactgaagg tcggtgtccc atctcccaca
    15781 acgattagga ttttttctgg atttctattt tctccgccat tgtctttata caatgtttca
    15841 tagccatttt taagccctag tgtaagcgga gttcctccga ttggtgtacg atttaatgct
    15901 gtattctcac ttcccgtcaa acgattgata tcatttgtca atggaaagat gtccgaactg
    15961 atttgctcat ggttattgat agctgtacta ttatggtaaa aattcaccat accgattcga
    16021 atatccagac tcgggtcaga gagcagatct ttactcattt cgtttactgc ctcaatcaac
    16081 gcagcatttt tagtttgact attggtccca gcggttaatt cactcatact cgctgactta
    16141 tctagaacaa aaacgatatc gattggactg atttcctttt tctcgccccc gatgacatct
    16201 agatagacat tgtaaagggt tgttgcatct tcaggatgta gatcagatgc tgtgctgctt
    16261 ggcagaacag gttctaccca ttttttgatg tacgccccgt tataatccaa gtaactgttt
    16321 tcaaaattcc gttcatttcc ccaaagctga acggtttgag ctggaacgtt tctatcttct
    16381 tcgcctgcag ttgatccatt attagtagca tagtcgtaat ttcgccagtt cgctcccccg
    16441 tttgtataat cactatcatc gatcaagttc tctccgtcat atggcgagcc tttgtctcct
    16501 tgatagcgtt cattgctttg tgtccaattt cctaacactt ttgtatagtc attcgttgta
    16561 aacttcgggt agcgatgctg ttcgttttca acttctccgc taggatagat atattcatag
    16621 gggtcttgcg tagctgttgg cccaagacgc tccaaaatac cgttgaataa ctggcgttga
    16681 atgcgctgga gtgcttgacc gctgggtacg attggttcgt ccagcttttc catctcattt
    16741 tcttcgctac gttgaatttc agcggaatct tctgtatcag tttcattttc ttgtaattct
    16801 tggttatcta ttctttcatc aaccaccact tgaagaggta gttacccttc gttcacatcg
    16861 gggatctttt ctgtttcaaa aatcaatgtg ccggtttctg cttcttttga ataaactttt
    16921 ccgacatacc attcttgacc atcgatcgtc tcgactttta ggtcttctcc atcttcatca
    16981 taggaggata gacctgttcc acgaacgttt tttacggtac caatcccggc ggctgccgca
    17041 tcgattgcta aacgtattct accttcattg gcactttcct ttttttcaaa agccagatgc
    17101 cattcaaggg tttcttcttt ttcaatatac ttcccttcaa tttttgcata gtcctcatct
    17161 aagaatacct catcctcctt tgtttctgtt gactcagcag ctacatccag ggtcgtcata
    17221 tgatagactg actggaaata tggtccaaat agcaaactga cagttactaa ctgcaaaagc
    17281 ttcaatttta accctttcac atccttatac tcctttcaaa aatatttgtc ttctttcaca
    17341 acaat
//
LOCUS       DAZKLD010000052        16999 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.052, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000052 DAZKLD010000000
VERSION     DAZKLD010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16999)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16999)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16999
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.052"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1152
                     /locus_tag="ACG8KR_001424"
     CDS             <1..1152
                     /locus_tag="ACG8KR_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HFQ0200801.1"
                     /translation="PSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHNSAPSTSAPSA
                     EAPKEKVETSGSASVVEAADPNKRVLAMPSVRQFAREKDVDISQVTATGKGGRVTKED
                     IENFLAGGPSSAPAKSEAPEAAAPKEAAPAAESKPAAPAKPFKSNLGDLEERVAMTPT
                     RKAIAKAMVNSKHTAPHVTLHDEVEVSKLWDNRKRFKEVAAANGTKLTFLPYVVKALT
                     ATVKKYPVLNASIDDANQEIVYKHYYNIGIATDTDHGLYVPNVKDADRKGMFAIADEI
                     NEKAKLAHDGKLSAEDMRNGTITISNIGSVGGGWFTPVINYPEVAILGVGTIAQQPIV
                     NAEGEIVVGRVMKLSLSFDHRIVDGATAQQAMNNIKRLLADPELLMMEG"
     gene            1160..2566
                     /gene="lpdA"
                     /locus_tag="ACG8KR_001425"
     CDS             1160..2566
                     /gene="lpdA"
                     /locus_tag="ACG8KR_001425"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HFQ0200802.1"
                     /translation="MVVGDFAVELDTVVIGAGPGGYVAAIRAAEMGQKVAIIEREYIG
                     GVCLNVGCIPSKALIAAGHHYQESLDSEMFGVTSENVKLDFAKTQEWKENKVVHTLTS
                     GVGFLLKKHKVETIEGEAFFVDDHTLRVIHPDSAQTYSFNNAIIATGSRPIEIPGFKF
                     GGRVLDSTGGLALKEVPKKFVIIGGGVIGAELGGAYANLGAEVTILEGSPQILPTYEK
                     DLVKLVEDDFKKKGVTVVTNAMAKEAVDNGDSVTVKYAVDGKEESVTADYVMVTVGRR
                     PNTDDMGLEQAGVEVGERGLITVDKQGRTNVPNIYAIGDIVPGAALAHKASYEAKIAA
                     EAISGKKVAVDYKAMPAVAFTDPELASVGMTIKEAKDAGIEAKAYKFPFSGNGRALSL
                     GKTEGFIRLVTTIEDNVLIGAQIGGVGASDMVSELALAIESGMNAEDIALTIHPHPSL
                     GEIVMDASELALGLPIHI"
     gene            2728..3003
                     /locus_tag="ACG8KR_001426"
     CDS             2728..3003
                     /locus_tag="ACG8KR_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HFQ0200803.1"
                     /translation="MQEYQYPLDLDWTTDEMVIVMKMWEDLERANEKGINNEAFLKTY
                     QQFKTVVKSIGEEKRLGREFEKASGYSLYRTVQQAKKNPNKTLRMNG"
     gene            3009..3776
                     /locus_tag="ACG8KR_001427"
     CDS             3009..3776
                     /locus_tag="ACG8KR_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HFQ0200804.1"
                     /translation="MEQMIDHIKSWLEEAGEKIKSSQQTLTISQKSNRTDLVTNVDRN
                     IQNFLIEKIHSFDPEAKILAEEEGCNQLSDLDGRVFIIDPIDGTLNFVIEGENFCIML
                     AVYESGVGKLGFIYDVMRNELFWGGKGIGVYKNDEPLPRPANKSLSEGLLGVNSYLFG
                     HNKYGIREIAESSMGTRMYGCAGLELVAILKGNHIAYISNLSPWDYAAGNVLLEEFGM
                     VTSGLFGTPLTFEKREYYLAGTSCAYAEIADLLKKEG"
     gene            3944..5773
                     /gene="typA"
                     /locus_tag="ACG8KR_001428"
     CDS             3944..5773
                     /gene="typA"
                     /locus_tag="ACG8KR_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HFQ0200805.1"
                     /translation="MNYRNDIRNVAIIAHVDHGKTTLVDELLKQSDTLDAHTQLQERA
                     MDSNALEKERGITILAKNTAVDYKGIRVNIMDTPGHADFGGEVERIMKMVDGVVLVVD
                     AYEGTMPQTRFVLKKALEQHITPIVVVNKIDKPSARPEHVVDEVLELFIELGADDDQL
                     DFPVIYASALNGTSSLSDDPADQEPTMAPIFDTIIEKIPAPVDNSDEPLQFQVSLLDY
                     NDYVGRIGIGRVFRGTIKVGDQVALIKLDGTVKKFRVTKLFGFFGLKRLEIQEAKAGD
                     LIAVSGMEDIFVGETVTPVDHQDALPILHIDEPTLQMTFLVNNSPFAGREGKFVTARK
                     IEERLMAELQTDVSLRVEPTNSPDAWTVSGRGELHLSILIENMRREGYELQVSRPEVI
                     EKEIDGVKCEPFERVQIDTPEEYMGSVIESLSLRKGEMQDMVHTGNGQIRLTFLTPAR
                     GLIGYSTEFLSMTRGYGIMNHTFDQYLPMLPGQIGGRHQGALVSIDTGKATTYSIMSI
                     EERGTVFVEPGTEVYEGMIIGENSRDNDLTVNITKAKQMTNVRSATKDQTSVIKKPKQ
                     LTLEESLEFLNDDEYCEVTPESIRLRKQILEKNAREKASKKKK"
     gene            6034..7806
                     /gene="recQ"
                     /locus_tag="ACG8KR_001429"
     CDS             6034..7806
                     /gene="recQ"
                     /locus_tag="ACG8KR_001429"
                     /EC_number="5.6.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HFQ0200806.1"
                     /translation="MNDLLKQYFGYDEFRPGQKEIIQKVIDQENVLGIMPTGSGKSIC
                     YQLPALLLDGLTVVVSPLISLMKDQVDAANQLGIPATFINSSLDGYETARRFQEIDRQ
                     QYRLLYIAPERFIMPDFIQAMKRWNVRMIAIDEAHCISQWGHDFRPSYLQMANQLDQL
                     PNRPVIVALTATATVQVAADIKRLLKIPENNHIQTGFERENLRFQVIKDQKKEQYLIE
                     YLKINKNQSGIIYAATRKEVDRLYHLLRKFDFSVGRYHGGLNENERTEMQEAFLYDRL
                     QLIVATNAFGMGINKSNVRFVIHYQIPGSLEAYYQEAGRAGRDGLSSEAILLFSPQDI
                     QVQKFFIQQSQREEGQKQKEYEKLKAMTEYVYIESCLQQYILNYFGETSSPCNRCGNC
                     LDDREIVEVTTEAQMVLSCLKRMGENYGKQMLMKVLAGSKEQKLQALGFGHLSTYGLM
                     KNQSQKETMQLIEYLISNGYLLTINGEYPVLKVTERGIQVLKGQESVYRKEPKKVQQL
                     SDEETDTLFEVLRELRTDLASEAGVPPYVVFSDSTLKEMSRIRPSSRLEMLQIKGVGQ
                     SKLDKYGEAFLSRIKNADSNVLDK"
     gene            7920..8222
                     /locus_tag="ACG8KR_001430"
     CDS             7920..8222
                     /locus_tag="ACG8KR_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HFQ0200807.1"
                     /translation="MDSLSKKLAIQVLNDEAERIKRLIRNQNNSLCISQCKAFEEVVD
                     TQMYGFSRQVGFAVRIGVIGKNEGQLMLSELERELNQLYSEVYQEIYDKKEVGKEE"
     gene            8225..9388
                     /locus_tag="ACG8KR_001431"
     CDS             8225..9388
                     /locus_tag="ACG8KR_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HFQ0200808.1"
                     /translation="MPKKVKKRHLLDYSILIPYLILCVLGLIMVYSSTSYLLLENGQN
                     PSASVINQSIFLVLSLIAIALLYKMKTDVLKNQRLIMAAIAVLTILLLIVVFFGKEIN
                     GAKGWLQIAGFSIQPAEYLKIISIWYLSLTLSKRQNSVQKDFLGTVKRPLAMVIGLTA
                     LVAILPDFGNAAVIFLIILVLLLASGVNYVYTLIVGVGGFCLSTFTIWLINITNGKIL
                     PGRLQYIYNRFAIYQNPFSDELNKGHQLVNGYYAMFNGGLFGRGLGNSIQKKGFLQEA
                     QTDFIYAIVVEELGVIMGILILALLFFMIVRIVLVGIRSKDPFNSLLCIGIGAMFLIQ
                     VFVNLGGITGVIPLTGITFPFLSQGGSSLLMLSICVGFVLNISADEKRKSLGL"
     gene            9414..12842
                     /locus_tag="ACG8KR_001432"
     CDS             9414..12842
                     /locus_tag="ACG8KR_001432"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004736 - pyruvate carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009374 - biotin binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HFQ0200809.1"
                     /translation="MKKVLVANRGEIAVRVFRACTELGIKTVGIYAEEDEYSVHRFKA
                     DEAYLVGQGKKPIDAYLDIEGIISIAKECGADAIHPGYGLLSENLNFAQRCEEEGIIF
                     VGPKLHHLDIFGDKIKAKAAAIEAGIASIPGTDGPIASIDDALEFAKQYGYPIMIKAA
                     LGGGGRGMRVAHDEKSAREGYERAKSEAKAAFGSDEVYVEKYIANPKHIEVQILGDTH
                     GNVIHLFERDCSVQRRHQKVVEVAPCVSMNEQQRQKICQAAVQLMKYVGYVNAGTVEF
                     LVEGDDFYFIEVNPRVQVEHTITEMITDIDIVTTQLLIAQGLDLHKEIGLPQQEGIKL
                     NGSAIQCRITTEDPLNNFLPDTGKIDTYRSPGGFGVRLDVGNAYAGYVVTPYFDSLLV
                     KVCTHGATFETAIQKMERCLKEFRIRGVKTNIPFMLNVITHPEFQSGNAKTTFIDSTA
                     TLFEFPRLRDRGNKTMKYIGEITVNGFPGIESGEKPFYEEPRMPKDLITRTDYVTAKN
                     VLDAKGADALVEWIKGQENLLLTDTTFRDAHQSLLATRVRTKDFKQIARLTGEGLPEL
                     FSSEMWGGATFDVAYRFLNEDPWQRLRKIRSLMPNTLLQMLFRGSNAVGYSNYPDNVL
                     VEFVKEAAAQGIDVFRIFDSLNWTPQMEKSIQAVRDTGKIAEAAICYTGDINDPSRAK
                     YNVQYYKDMAKELEQLGAHIIAIKDMAGLLKPQAAYRLISELKETTDLPIHLHTHDTS
                     GNGIITYSAASKAGVDIVDVAMSAMSGNTSQPSMSSLYYALVNGPRLPEITIENAQKL
                     NHYWEDVRMYYKPFENGLNAPETEVYMHEMPGGQYSNLQQQAKAVGLGHRWDEIKQMY
                     HTVNLMFGDIVKVTPSSKVVGDMALFMVQNDLTEEDIYEKGETLSFPESVVTFFQGEL
                     GQPVGGFPKKLQKIILKGRPALNERPGLLAESVDFNEVKKELAEKIGYEPKQDEVLSY
                     LMYPQVFLDYQKAYNQFGDVTLLDTPTFFQGIRLGETINVQIERGKTLIIRLDEIGEP
                     DIEGNRVLFFNLNGQRREITIKDNSIISTVQTRLKAEPTNREQIGATMSGSVLDVLVK
                     KGDNVKKGDTLMITEAMKMETAIEARFDGEVAHVYVSSGDTISSGDLLIEVTEK"
     gene            12863..13987
                     /locus_tag="ACG8KR_001433"
     CDS             12863..13987
                     /locus_tag="ACG8KR_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP-associated domain-containing protein"
                     /protein_id="HFQ0200810.1"
                     /translation="MKRLGLFWLILLLVIVAFYMEPVLFPPKLEVKENSDQQISNQMT
                     TWKYQELKATGFSTYIGKPIDQLEKDFGQAYDRLTSGFGFETRYYENKASHLTFEANI
                     QDSRVTTVKVLQTGPADIAPFYLGMTMQDLTKITTIYTNFTFEYHDTEVGIELMEEDM
                     NYRPLIAFDNDTFAILFFDQTTNGLFSVVYLDKDSLLKLLPYQVFGEGLPHYQADKSA
                     DWESINRVKERKSTTLLNMLRREDELPIYNQTLAFQNHSQLLLHDFLTRPEEILSEER
                     MADWQKSLGSAQTTVKFSLTNDELEKLISKQKLENTSGLFIHPAIDATFSFLYWYSDP
                     YNHDRFMDPGTDSVGIAFSKENMLVLLQEENGESSDSSDR"
     gene            13968..14414
                     /locus_tag="ACG8KR_001434"
     CDS             13968..14414
                     /locus_tag="ACG8KR_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HFQ0200811.1"
                     /translation="MIAVIVNEELFQLEDQCLQVAEMIKKSKTMASYLEHKQSMEESA
                     KVCQLKEAFLSKKEAFENVAAYGEYAPDYREKKRAVRKAKRQLDMDEHVAAFRYSETQ
                     LQSLLDQIVQVLAEEVSNEIKVDAGSPFFETKKHFGCGGNCHAIRQ"
     gene            14398..14736
                     /locus_tag="ACG8KR_001435"
     CDS             14398..14736
                     /locus_tag="ACG8KR_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HFQ0200812.1"
                     /translation="MQLDNSEFEITKRRGLIVWVYSLKQLKNLKKFGLLHYVSRRMKY
                     VVLYLNEETLEETEERIRKLHFVRNVERSYRPDIEMNFAEKIGNKTVLKDEGFEIEEL
                     NTEIRLAEHL"
     gene            14855..15415
                     /gene="rsmD"
                     /locus_tag="ACG8KR_001436"
     CDS             14855..15415
                     /gene="rsmD"
                     /locus_tag="ACG8KR_001436"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HFQ0200813.1"
                     /translation="MRIISGNYGGRRLKSLAGANTRPTTDKVKESIFNMIGPYFDGET
                     VLDLFAGSGGLAIEAVSRGCSHAVCVDKNYQALKIIKENIEITKEPEKFTTIKLDADK
                     AIERLAAEKQQFDYLFLDPPYAKQKIIDQIHKMEELGLFSKTAVIVCETDKTVELPET
                     VAGFHQIRKQIYGITAVTIYRKEGNQ"
     gene            15412..15903
                     /gene="coaD"
                     /locus_tag="ACG8KR_001437"
     CDS             15412..15903
                     /gene="coaD"
                     /locus_tag="ACG8KR_001437"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HFQ0200814.1"
                     /translation="MKRALFPGSFDPFTKGHLDTVERAAKLFDEVVIGVFINTSKKSL
                     FPPEERMTLITKAVSHLPNVKVMHQENQLTVETAKEIGADALIRGIRSIKDFEYEREI
                     AQMNHHLYDELETVFLLAKPEYSHVSSSILKEVLHFGGDVSSYLPPVINEALAGKRES
                     NES"
     gene            15893..16936
                     /locus_tag="ACG8KR_001438"
     CDS             15893..16936
                     /locus_tag="ACG8KR_001438"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SepM family pheromone-processing serine
                     protease"
                     /protein_id="HFQ0200815.1"
                     /translation="MKAKQWLKTVLIIIFGMLIIGFVVVPLPYYVESPGATIDLKELI
                     TVNDKEDKGSGSFSLTSVGIRQATGFLALKAKMTPFEELISEEELTGGATNEEYNQMQ
                     QYYMDSSQNAAIEQALKLADKPYKMEFKGVYVMSVEKTSNFFGKLSVGDTVTKVDGKS
                     FQSTEAFMDYVKSQKVGQTIEISYLHNGEEKTASGDLIELPTDKKAGIGITLTDHTEI
                     ESDTDVRINSGSIGGPSAGLMFTLEIYEQVTGKNLRHGKQIAGTGTINSEGEVGRIGG
                     IDKKVASADKAGVEIFFAPDDTISDDVKKEYPEIKTNYEEAKAAAKKLNSSMKIVPVK
                     TVQEALDYLEKMK"
BASE COUNT         5754 a         2945 c         3751 g         4549 t
ORIGIN      
        1 ccgtcacctg tcacagggac agttaaaaac gtgattgttc cagaaggcac tgtagcaaat
       61 gttggggatg tattagtaga aatcgatgct cctggacaca atagtgcacc gtcaactagt
      121 gcgccatcag ctgaagcacc aaaagaaaaa gtggaaacat caggttcagc aagtgtcgtt
      181 gaagctgccg atccaaataa acgtgtattg gctatgccat ctgttcgtca atttgcccgt
      241 gaaaaagatg tagatatctc acaagtaact gcaactggaa aaggtggcag agtaacaaaa
      301 gaggacatcg aaaacttctt ggcaggtggt ccaagcagtg caccagcgaa atcagaagcg
      361 ccagaagcgg cagcaccgaa agaagcagct cctgcagcag aaagcaaacc agctgcacca
      421 gcaaaaccat tcaagtccaa cttaggagac ttggaagaac gtgtagcaat gacgccaaca
      481 cgtaaagcta tcgctaaagc aatggtcaac agtaagcata cagctccaca cgtgacattg
      541 catgatgaag tagaagtgtc taaactatgg gataatcgga aacgatttaa agaagttgct
      601 gctgcaaatg gtacgaaact tactttcttg ccatatgtcg tgaaagcttt aacagcgact
      661 gttaagaaat acccagtatt gaatgcatct atcgacgatg caaaccaaga aatcgtttat
      721 aaacattact acaatatcgg tattgcaact gatacggatc acggactata tgtaccaaat
      781 gtgaaagatg cagaccgcaa aggaatgttt gctattgcag acgaaatcaa tgagaaagca
      841 aaattagctc atgacggcaa actatctgca gaagatatgc gcaatggaac aattacgatc
      901 agcaatatcg gatctgtagg tggcggttgg tttacaccag tcatcaacta tccagaagta
      961 gctattttag gtgtaggaac gattgcacaa caaccaatcg taaatgctga aggcgaaatc
     1021 gttgtcggtc gtgtgatgaa actgtcatta agtttcgatc accgtatcgt agatggagca
     1081 actgcgcaac aagcaatgaa taatatcaaa cgtcttttag ctgatccaga gctattaatg
     1141 atggaaggat gataaacaaa tggtagttgg agattttgct gtagaattag atacagttgt
     1201 aattggtgcg ggacctggtg gctatgtagc tgccatccgc gctgctgaaa tgggacaaaa
     1261 agtagcaatc attgaaagag agtatattgg cggtgtttgt ttgaatgtcg gctgtattcc
     1321 ttcaaaagca ctgatagctg caggacacca ttatcaagag tcactagatt ctgaaatgtt
     1381 tggagtaact agtgaaaacg tcaaactaga ttttgcaaaa acacaagaat ggaaagaaaa
     1441 caaagttgtc catacgttga cttctggtgt tggtttccta ttaaaaaaac ataaggttga
     1501 aacaatcgaa ggagaagcgt tcttcgtaga tgatcacaca ctacgcgtca ttcatccaga
     1561 ttcagctcaa acttattcgt tcaataacgc aatcatcgca acaggttccc gtccaatcga
     1621 gattccagga tttaaattcg gaggacgtgt gttagattct actggcggat tagcattgaa
     1681 agaagtacct aagaaatttg tgatcatcgg tggcggtgtg attggtgccg aattgggcgg
     1741 agcgtacgca aacttaggag cagaagtaac gattcttgaa ggctcaccac aaattttacc
     1801 aacttacgaa aaagatctag taaaattagt agaagatgat ttcaagaaaa aaggcgtaac
     1861 agtcgtaacg aatgcaatgg cgaaagaagc agtcgataac ggtgacagcg tgacagtgaa
     1921 atatgcagtg gacgggaaag aagaatccgt tactgctgat tatgtgatgg tcactgtagg
     1981 tcgtcgtccg aatacagatg atatgggctt agaacaagcg ggtgtcgaag taggagaacg
     2041 aggattgatc acggtggata aacaaggacg tacgaacgta cctaacatct atgccatcgg
     2101 tgatatcgtt ccaggggcgg cattagcaca caaagccagt tatgaagcaa aaatcgctgc
     2161 tgaagcaatc tctggcaaaa aagtagctgt agattataaa gcaatgccgg cagtagcctt
     2221 cactgatcct gaattggctt ctgtaggtat gacgatcaaa gaagcaaaag atgctggtat
     2281 cgaagcaaaa gcatataaat tcccattttc agggaacggt cgggcattat cgcttggtaa
     2341 aacagaagga ttcatccgtc tagttacgac gatcgaagat aacgtgctta ttggcgcgca
     2401 aatcggtgga gtcggcgcaa gtgatatggt ctctgaatta gctttagcaa tcgaatcagg
     2461 aatgaatgct gaagatatcg ccttgacgat tcatcctcat ccatctcttg gcgaaatcgt
     2521 catggatgct tcggaattag cattaggttt accaatccat atttgatttg aataatacag
     2581 gcgaaacgca actgaaaaat cagttgcgtt ttgtttttta gaattatcaa taatattatt
     2641 atgtaaactt aaaaatatga caacttcttg gtttattacc acttcctatg ctacactgaa
     2701 gaggaatatg aaataggaga gttgaagatg caagaatatc agtatccact tgatttagat
     2761 tggacaacag acgaaatggt cattgtaatg aagatgtggg aagatctgga acgtgcgaat
     2821 gaaaaaggga taaacaatga agcttttttg aagacttatc agcagtttaa aactgttgtc
     2881 aaatccattg gtgaggaaaa acgattggga cgggaatttg aaaaagcttc cggttattct
     2941 ttatatcgta cagtgcagca agcaaaaaaa aatccaaaca aaactttgag aatgaacggg
     3001 tgagataaat ggaacagatg attgatcata tcaagtcctg gcttgaagaa gctggtgaga
     3061 aaatcaaatc ttctcaacaa acattaacaa tctcgcaaaa atcaaatcga acggatctag
     3121 taacaaatgt cgatcgaaac atccagaatt ttctaatcga aaaaatccat tcctttgatc
     3181 cagaggcgaa aatattggca gaagaagaag ggtgtaatca attatcggat ctcgatggaa
     3241 gagtctttat catcgatcct attgatggca ccttgaattt tgtgattgaa ggggaaaatt
     3301 tttgtattat gctcgctgtt tatgaatccg gcgttggaaa gcttggtttc atttatgacg
     3361 tgatgagaaa tgaacttttt tggggtggaa aaggaatagg cgtgtataaa aatgatgagc
     3421 cattgcctag accagcaaat aaatccttat cggaaggctt acttggtgtg aatagttatc
     3481 ttttcggtca taataaatat ggaatccgag aaattgcgga aagcagcatg gggacacgta
     3541 tgtacggatg cgctggtctt gaactagtgg caatcttgaa aggaaaccac attgcttata
     3601 tttcaaacct tagcccttgg gattatgcag cgggaaatgt cttattggaa gagtttggta
     3661 tggtaacaag cggtcttttt ggtactcctt tgacatttga aaaaagggaa tactatttag
     3721 cagggacttc ttgtgcatat gcagaaatcg cagacctttt gaaaaaagaa ggctgaaaaa
     3781 aaggtagaaa tcattcacaa aaatgataaa aacgagcaaa agattaattt tctcgaaaaa
     3841 atgaaaacat tttactatat ttcatgaaaa aagtctgcta taattagcag tcgagtaaaa
     3901 tttgccctgt cataaagaac caataaaaaa ggagttagtt acattgaatt atagaaacga
     3961 tatccgtaac gtcgcaatta ttgcccacgt tgaccacgga aaaacaacat tagttgatga
     4021 actattaaaa caatcagata cattagacgc acatacgcaa ttgcaagaac gtgcgatgga
     4081 ctcaaatgcg ctagaaaaag aacgtggtat cacgatttta gccaaaaaca cagctgtaga
     4141 ctataaaggt attcgcgtaa acatcatgga tactccagga cacgcggact tcggtggcga
     4201 agtagaacgg atcatgaaaa tggttgacgg cgttgtgttg gtcgtagatg cgtatgaagg
     4261 aacaatgcct caaacacgtt ttgtattgaa aaaagcactg gaacaacata tcacaccaat
     4321 cgttgtcgta aacaagattg acaaaccttc tgctcgtcct gagcatgtcg tagatgaagt
     4381 tcttgaacta ttcatcgaat taggagcaga tgatgatcag ttagactttc ctgtgattta
     4441 tgcatctgca ctaaatggta cgtcaagctt gtcagatgat ccagcagatc aagaaccaac
     4501 catggcacct atttttgata caattattga aaaaatccct gctcctgtcg ataacagcga
     4561 cgaaccatta caattccaag tttcattact tgactacaac gattatgtag gtcgtatcgg
     4621 aatcggacgt gtatttagag gaacaatcaa agtaggagac caagtagcat taattaaatt
     4681 agacggaaca gttaagaaat tccgtgtaac gaaattattt ggtttctttg gactaaaacg
     4741 tctagagatt caagaagcaa aagctggaga cttgattgct gtctcaggta tggaagatat
     4801 ctttgtcggt gaaacagtta ctcctgtgga tcatcaagat gcgttaccta tccttcacat
     4861 tgacgaacca acattgcaaa tgactttctt agttaataat tcaccatttg ccggtcgaga
     4921 aggtaaattc gtaaccgctc gtaagattga agaacgtttg atggcagaac ttcaaacaga
     4981 tgtatcctta cgagttgaac caacaaactc tccagatgca tggacagttt ctggtcgtgg
     5041 agaattgcat ttgtctatct tgattgaaaa catgcgccgt gagggatacg aattgcaagt
     5101 atctcgtcca gaagttattg aaaaagaaat cgacggtgta aaatgcgaac cgtttgaacg
     5161 cgtgcaaatc gatacaccag aagaatatat gggtagtgtt atcgagtcct tgagcttacg
     5221 taaaggtgaa atgcaagata tggtccatac aggaaacgga caaatccgtt tgacattctt
     5281 aacacctgcc cgcggtttga ttggttattc aactgaattc ctttcaatga cacgtggtta
     5341 cggaatcatg aaccatactt ttgaccaata tctgccaatg cttcctggtc aaattggcgg
     5401 acgtcaccaa ggggcgcttg tttcgatcga tacaggaaaa gcaacgactt attctatcat
     5461 gagtatcgaa gaacgcggta cagtcttcgt agaaccaggt acagaagtat atgaaggaat
     5521 gattatcggg gaaaacagcc gtgataatga tttgactgtc aacatcacga aagcaaaaca
     5581 aatgacaaac gtacggtctg ctactaaaga ccaaacatct gtcatcaaaa aaccaaaaca
     5641 attgacgttg gaagaatctt tggaattctt gaatgatgat gaatattgcg aagtaacacc
     5701 agaaagcatt cgcttgagaa aacaaatttt agagaaaaat gctcgtgaaa aagcatctaa
     5761 aaagaaaaaa taatctgtaa aaggatgaag agagaaacca gcttgttata aaaaatagct
     5821 ggtttctttt tttgtccaac tcttttctgt ggagaagtca tctttgtcaa tggacataaa
     5881 ttcagaaaat acgccgaaag acgtttaaaa gacttttaaa aaacttattt ctagagagtt
     5941 tttgtttcat aataagataa tgtttcacga aaggagatca acttgtcctt cgaagaaaat
     6001 cgaagtaaaa tggcaagaga aaggagcggg aagatgaacg acttattaaa acaatacttt
     6061 ggctatgatg agtttagacc tggtcaaaaa gaaattatcc agaaagtcat tgatcaggaa
     6121 aatgtattag gaatcatgcc taccggaagt gggaaatcaa tctgttacca gcttccagct
     6181 ttactattag atgggcttac tgtggttgtg tcccctttaa tctctttgat gaaggatcag
     6241 gtggacgcgg cgaatcaatt aggtataccc gccactttta tcaatagctc gttagatggt
     6301 tacgaaacag ctagacgctt ccaagaaatc gatagacagc agtaccgttt actttatatc
     6361 gctcctgaac gttttatcat gccggatttt atccaagcaa tgaagcgatg gaacgtccgt
     6421 atgatcgcta ttgacgaggc acattgtatc tctcagtggg ggcatgattt ccgcccaagt
     6481 tatttacaga tggcgaacca gctagatcaa ctgccaaatc gtcctgtaat tgttgctttg
     6541 acagctacag cgactgtgca agtagctgct gacataaaaa gacttttaaa aattccagaa
     6601 aacaaccata ttcagactgg ttttgaacga gaaaatctac gatttcaagt aattaaagat
     6661 caaaaaaaag aacaatatct aatcgaatat ttaaagatca acaaaaatca atctggtatc
     6721 atttatgcag caacgagaaa agaagtagac cgtctctatc atttgcttag aaaattcgat
     6781 ttttccgtgg gtagatatca tggtggtcta aatgagaatg agcgaacaga aatgcaagaa
     6841 gcctttttat acgatcgctt gcagctgatt gtagcgacaa atgcatttgg tatgggtata
     6901 aataaaagca atgtccgttt tgttatccat tatcaaattc ctggctctct agaagcatac
     6961 tatcaggaag ctggtcgggc gggaagagat ggtctttcca gtgaagccat cctgcttttc
     7021 tctcctcaag atatccaagt ccaaaagttt tttatccagc agtcccaaag agaagaaggg
     7081 caaaagcaaa aagaatatga aaaattaaaa gcgatgacag aatacgtgta tattgagtcc
     7141 tgtcttcagc aatacatcct aaactatttt ggcgagacaa gcagtccgtg caaccgttgt
     7201 ggtaactgtt tagatgacag agaaatagta gaagtaacga cagaagcaca gatggttctt
     7261 tcttgcttaa aacgtatggg tgagaactat ggtaaacaaa tgctgatgaa agtcttagca
     7321 ggatcgaaag aacaaaagct gcaagcttta ggttttggac atttatctac ctatggactg
     7381 atgaaaaacc aatcgcaaaa agaaacgatg cagttaattg aatatttgat atcgaatggc
     7441 tatcttctga ccatcaatgg tgagtatcct gttttgaaag taacagaaag agggatacaa
     7501 gtattgaaag gacaagaatc tgtttatcga aaggaaccca agaaagtcca gcagctatcg
     7561 gatgaagaaa ccgatacact atttgaagtg ctgcgggaac tgcgaacgga tctagcatca
     7621 gaagcaggcg ttcccccgta tgtcgtattt tcagattcca cattgaaaga aatgagccga
     7681 atacgtccat caagcagatt ggagatgctt cagataaaag gagttggcca aagcaagctt
     7741 gataaatatg gcgaagcctt tttatctcgg ataaaaaatg cggactctaa cgttctggat
     7801 aagtagaaag aacgaatacg taaaaatgaa taaaaacaac tggttaaatc tgtttttttt
     7861 gctccttatg aaaatcaaat atgttataat taaggaatta gaatgggagg taagcacaga
     7921 tggattcttt gtcgaaaaaa cttgcgatcc aagtattaaa tgatgaagca gaacggatca
     7981 aacgattaat ccgaaatcaa aacaacagtc tctgtatctc acaatgcaaa gccttcgaag
     8041 aagtggtaga tacacagatg tacggctttt cccggcaagt cggttttgct gtccgtatag
     8101 gagtaatcgg aaagaacgag ggccagctga tgttaagtga gctagaacga gaattgaatc
     8161 agttatatag tgaggtttac caagaaattt atgataaaaa agaagttggc aaggaggaat
     8221 agacttgcct aagaaagtga aaaaaaggca tctattagac tatagcatct tgattcccta
     8281 tttaatttta tgtgtattgg gattgatcat ggtatatagt tctacgtcgt atctattatt
     8341 agaaaacgga caaaatccgt ccgcatcagt cattaaccag agtatttttt tggtgcttag
     8401 cttgattgct atcgcactac tttataaaat gaaaacagat gttttgaaaa accagcgttt
     8461 gatcatggca gccatcgcag ttttgacgat tttgctgctg attgttgtgt tttttggtaa
     8521 ggagatcaat ggggcgaaag gttggctgca aattgcagga ttctcgatcc aacctgccga
     8581 atacctaaaa atcatttcta tctggtacct gtctctgact ttatctaaaa gacaaaacag
     8641 tgtacaaaaa gattttcttg ggactgtgaa acgtccactt gcaatggtta tcggacttac
     8701 tgcattagtc gctattcttc ccgattttgg taatgcggca gttatctttt tgattatttt
     8761 ggttctgctt ttagccagcg gagtcaatta tgtgtatact ttgattgtag gagtaggagg
     8821 cttttgtcta agtactttta cgatctggct gatcaatatc acaaacggaa agatcttacc
     8881 cggacgttta cagtatatct ataatcgatt tgctatttac caaaatcctt tctcagacga
     8941 attaaataaa gggcatcaac tagtcaacgg ctactatgcc atgttcaacg gaggactatt
     9001 tggcagggga ttaggtaaca gtatccagaa aaaaggattt ttacaagaag cacaaaccga
     9061 ttttatttat gcaatcgtag tggaagaact gggagtgatt atggggattt tgatattagc
     9121 gttgctattc tttatgattg tgcgcattgt ccttgtaggt atccgttcaa aagatccgtt
     9181 caattcgctt ttgtgcattg ggatcggcgc aatgtttttg atccaagtat ttgtcaatct
     9241 tggagggatt actggcgtta ttcctttgac agggatcacg ttcccatttt taagtcaagg
     9301 aggctccagt ttgctgatgc tttcgatttg tgtcggcttt gtcctcaaca tcagtgcgga
     9361 tgaaaaacgc aagagcttag gtttataata aaaaaagatg taggagtggt tcgatgaaaa
     9421 aagttttagt tgctaacagg ggtgaaatcg ctgtacgtgt atttcgtgct tgtacagaat
     9481 taggaattaa aactgttgga atctatgcag aagaagatga atattccgta caccgtttca
     9541 aagcggatga agcatattta gtaggacaag gaaaaaaacc gattgatgcg tatctagata
     9601 ttgaagggat catttctatc gcgaaagaat gcggagctga cgctattcat ccgggttacg
     9661 gtcttttgtc ggaaaacttg aattttgcgc aacggtgcga agaagaaggc atcatttttg
     9721 ttggacccaa actgcatcat ttagatattt ttggagataa aatcaaagca aaagctgcag
     9781 ctatcgaagc ggggattgct tcgattccag gcacagatgg accaatcgct tctatagacg
     9841 atgccttgga gtttgcgaaa caatacggtt atccgattat gatcaaggca gctctaggcg
     9901 gcggcggacg cggcatgcgt gtagctcacg atgaaaagag tgcaagagag ggctacgaac
     9961 gtgcgaaaag cgaagcaaaa gcagcctttg gtagtgatga agtgtatgtt gaaaaataca
    10021 tcgctaatcc taagcatatc gaagtacaga ttctcgggga tacgcatgga aacgtcatcc
    10081 atctttttga aagagattgt tctgttcagc gccgtcacca aaaagtagta gaagtagcgc
    10141 cttgtgtctc tatgaatgaa cagcaacgtc aaaaaatctg ccaggcagct gtccaattga
    10201 tgaaatatgt cgggtatgtc aacgcaggta cagtagaatt tcttgttgaa ggagatgatt
    10261 tttactttat cgaagtcaac cctcgcgtcc aagtggaaca tacgatcaca gaaatgatta
    10321 cagatattga tatcgtaaca acgcaactgt tgattgctca aggtttggat ctgcataaag
    10381 aaatcggatt gcctcagcaa gaaggaatta aattaaatgg ttcagccatt caatgccgaa
    10441 tcactacaga agacccgttg aataactttt taccagacac agggaaaatc gatacgtatc
    10501 gttcacctgg tggttttggc gtacgtctgg atgtcggaaa tgcttacgcg ggctatgtag
    10561 tgacaccgta ttttgattca ttgctagtaa aagtatgtac gcatggtgct acctttgaaa
    10621 cagccattca gaaaatggaa cgttgcttga aagaatttag aatccgtgga gtgaaaacaa
    10681 acattccatt tatgctcaat gtcatcacac atcctgaatt ccaatcagga aatgcaaaaa
    10741 caaccttcat tgatagtaca gcaacattat ttgaatttcc tcgactacga gaccgcggaa
    10801 acaaaacgat gaaatatatc ggtgaaatta ctgtgaatgg ttttccaggt atcgaaagtg
    10861 gcgaaaaacc attttatgaa gaaccacgta tgcctaaaga tctaatcaca agaactgatt
    10921 acgtaacggc taagaatgta ttagatgcga agggtgccga tgctttggtc gaatggatca
    10981 aaggacagga aaatttgttg ttgacggata cgacatttag agacgcccat caaagtctgc
    11041 tagcaactcg tgttcggaca aaggatttca agcagattgc acgtttgact ggagaaggtc
    11101 ttcctgaact cttttcaagt gagatgtggg ggggagcaac atttgatgtg gcttaccgct
    11161 ttttgaatga agatccttgg caacggttga gaaaaattcg ttctttaatg cctaatacgt
    11221 tgctgcaaat gcttttcaga ggttcaaatg ccgttgggta ttctaattat cctgacaatg
    11281 tattggtgga atttgtcaaa gaagcagcag cgcaaggaat cgatgtattt cgaatctttg
    11341 atagtttgaa ctggactcca caaatggaaa aaagtatcca agctgtacgg gatactggta
    11401 aaatcgcaga ggcagctatt tgctatactg gcgatatcaa cgatccaagt cgggcaaaat
    11461 acaatgttca gtattataaa gacatggcaa aggaactaga acaactgggt gcgcatatca
    11521 ttgcaatcaa agatatggct gggctgctta aaccgcaggc agcttatcgt ttgattagtg
    11581 aattgaaaga aacgacagat ctgccaatcc atctgcatac tcatgatacc agcgggaatg
    11641 gaatcatcac ttattctgct gcttcaaaag caggagtaga catcgttgac gtggcaatga
    11701 gtgcaatgag tgggaatact agtcagccta gcatgagcag cttgtattat gcactggtca
    11761 atggtccacg gctgccagaa atcacgattg aaaatgcaca gaaattaaac cattattggg
    11821 aagatgtgcg catgtattac aaaccgtttg aaaacggctt gaatgctcct gaaacagaag
    11881 tatatatgca tgaaatgcca ggaggacagt attcgaatct acagcaacag gcaaaagctg
    11941 tcggattagg tcatcgctgg gatgaaatca aacaaatgta tcatactgtc aacttgatgt
    12001 ttggcgatat cgttaaagta actccatcat ctaaagtagt tggagatatg gcactattta
    12061 tggtccaaaa tgatcttaca gaagaagata tttatgaaaa aggcgaaaca ctaagtttcc
    12121 ctgaatctgt tgtgactttc ttccaaggag aactagggca acccgttggc ggatttccta
    12181 aaaaattgca aaagattatt cttaaaggca gacctgcatt gaatgaacgt ccaggtcttt
    12241 tggctgaatc agtcgacttc aacgaagtaa aaaaagaatt ggcagaaaaa attggctatg
    12301 aaccaaaaca agacgaagta ttaagctatt tgatgtatcc gcaagtcttt ttggactatc
    12361 aaaaagcata taatcaattt ggcgatgtta cattactgga cacaccaaca tttttccaag
    12421 gtatacgttt gggtgaaaca atcaatgtac aaatcgaacg cggaaaaacg cttatcattc
    12481 gtttagatga aatcggagaa cccgatatcg aagggaatcg cgtactgttc ttcaatttga
    12541 atggacaaag acgtgaaatc actatcaaag ataattcgat catcagtacg gtccaaaccc
    12601 gattgaaagc tgaaccgaca aaccgagaac aaattggagc aacgatgtct ggttctgttt
    12661 tggatgtatt agtgaaaaaa ggcgataacg ttaaaaaagg tgatacgttg atgattactg
    12721 aagcgatgaa aatggaaacg gccatcgaag cacgttttga cggagaagtc gcacatgtat
    12781 atgtgtcttc aggagacact atttcgtctg gtgacctttt gattgaagtg actgagaaat
    12841 aataagcgaa aggtggggaa acatgaaaag actgggtttg ttttggctga ttctgctatt
    12901 agttattgtg gcattctaca tggaaccagt cttgtttccg ccaaaacttg aagtgaagga
    12961 aaactctgat caacaaatat caaaccaaat gacaacgtgg aaataccagg agcttaaagc
    13021 aactggtttt tctacttata tcggaaaacc gatcgatcag ctggaaaagg attttggcca
    13081 agcctatgat cgattaacta gcggatttgg ttttgaaaca agatattatg aaaataaagc
    13141 tagtcatctc acatttgaag cgaacatcca agacagcaga gtcacaactg ttaaagtact
    13201 gcaaacaggg cctgctgata tcgcgccgtt ttatttaggg atgacgatgc aagatcttac
    13261 gaaaatcact actatctata ctaatttcac ttttgagtac catgatacag aagttggaat
    13321 cgaactaatg gaagaggata tgaattatcg tccgctaatt gcttttgata acgacacttt
    13381 tgctatttta ttttttgatc aaacaacaaa cggcctattt tcagttgtgt atttagataa
    13441 agatagtctt ttgaaactat tgccatatca agtgtttggt gaaggccttc cacattatca
    13501 agcagataaa tcggctgatt gggaatcaat caatcgcgtt aaagaacgaa aaagtacgac
    13561 tttattaaat atgcttagaa gagaagacga acttcccata tacaatcaaa cacttgcctt
    13621 tcaaaaccac agtcagctgc tactacatga ttttttgaca aggcctgagg aaatattgtc
    13681 agaggaacgt atggctgatt ggcaaaaatc gctaggcagt gctcaaacga cagtgaaatt
    13741 ctcactgacg aatgatgaat tggagaagtt aatcagtaaa caaaaattag aaaatactag
    13801 tgggctattt attcatccgg cgattgacgc tactttttcg tttttatatt ggtacagcga
    13861 tccttacaac catgaccgtt tcatggaccc gggaacggat tctgtaggca ttgctttttc
    13921 taaagaaaat atgttagtct tattgcaaga agaaaacgga gaaagtagtg atagcagtga
    13981 tcgttaacga agagctgttt caactggagg atcaatgcct ccaagtcgct gaaatgataa
    14041 aaaagagtaa aacgatggct tcttatcttg aacataaaca gtcaatggaa gaatctgcaa
    14101 aagtttgcca gttgaaagaa gcgttcttgt ctaagaaaga agcatttgaa aatgtagcgg
    14161 catatggtga gtatgcacct gattatcgtg aaaaaaaacg agctgttcga aaagcaaaaa
    14221 gacagcttga tatggatgag catgttgcag ctttccgata tagtgaaaca cagctgcaaa
    14281 gtctgttgga tcaaattgtc caagtacttg ccgaagaagt ttctaatgaa atcaaagtag
    14341 atgcaggtag cccgtttttt gaaacgaaaa aacactttgg atgcggaggt aattgtcatg
    14401 caattagaca atagtgaatt tgaaataaca aaacgacgcg ggctgatcgt atgggtctac
    14461 agcttaaaac aattaaagaa tctgaaaaag ttcggcttgt tgcattatgt ctcaagaaga
    14521 atgaaatatg tcgtgcttta cttaaatgaa gagacattgg aagaaacaga agaacgaatc
    14581 cgtaaattac attttgttcg aaacgtagaa cgttcttacc gaccggatat cgaaatgaat
    14641 tttgccgaaa aaataggaaa caaaacagta ttaaaagacg aaggtttcga aattgaagag
    14701 ctaaatacag aaatccgatt agctgaacat ctttaaaaat ctcttatgag cagtttttaa
    14761 ataaaaaata acttggataa cgatcgattt ttgatcattg tccaagtatt tttttggtat
    14821 actagtacca gtaccatttc taaggagtga gtagatgcgg ataatatcag gaaattatgg
    14881 cgggcgccgt ttaaaaagtt tagcaggggc gaacaccaga ccaactacag ataaagtaaa
    14941 agaatccata ttcaatatga ttggaccata ttttgatgga gagacagtat tggatctgtt
    15001 tgcaggaagc ggtggtttag ctatcgaagc agtatctaga ggctgcagtc atgcggtatg
    15061 cgtagacaaa aattaccaag cgctaaaaat catcaaagaa aacatcgaaa ttacgaaaga
    15121 accagagaaa tttaccacaa taaaacttga tgcggacaaa gcaatcgaac ggctagcagc
    15181 ggagaaacaa caattcgact acttgtttct tgatccgcca tatgcaaaac aaaaaataat
    15241 cgatcaaatc cataaaatgg aagaactggg acttttttct aaaaccgcag ttatcgtatg
    15301 cgaaacggat aaaacagtcg aattgccaga aactgtggct ggattccatc aaataagaaa
    15361 acagatttat ggcataacgg ccgtaactat ctatagaaaa gagggaaatc aatgaaacgt
    15421 gcattattcc caggaagttt tgatcctttt acaaaaggtc acttagatac ggtggaacgt
    15481 gcggctaaat tattcgatga ggtagtaatt ggtgttttta tcaatacttc aaagaaaagt
    15541 ttgtttcccc cagaagaaag aatgacattg atcacaaaag ctgtttctca tcttcctaat
    15601 gtaaaagtca tgcatcaaga aaatcaattg acggtagaaa cagcaaaaga gattggtgca
    15661 gatgcgttga tcagaggcat acgaagtatc aaagactttg aatatgaacg agagattgct
    15721 cagatgaatc atcatctata tgatgaattg gaaactgtct tcttattagc aaaaccagaa
    15781 tatagtcacg tgagttccag tattttaaaa gaagtactgc actttggcgg agatgtgagc
    15841 agctacttac cgccagtgat caacgaagct ttagctggaa agcgtgaatc aaatgaaagc
    15901 taaacaatgg ttgaaaacag tactgattat catatttgga atgttgatca ttggatttgt
    15961 agtagttccc ttgccgtatt atgtagaatc accgggagcc acgatcgatc tgaaagaact
    16021 aattacggtc aatgataagg aagataaagg ttctgggtct ttctctctta cctctgttgg
    16081 tatcaggcaa gctacaggtt tcttagcttt aaaagctaaa atgacccctt ttgaagaatt
    16141 gatcagtgaa gaagaattga ctggtggggc gacaaatgaa gaatataatc aaatgcaaca
    16201 gtattatatg gattcttccc aaaatgcagc aatcgaacaa gcgttaaaac ttgcagacaa
    16261 gccttataaa atggaattca aaggcgtata tgtcatgagt gtagagaaaa catcgaattt
    16321 ctttggcaag ctttcagttg gagatacagt aacaaaagta gacgggaaaa gctttcaaag
    16381 tacagaagca tttatggatt atgtaaaaag ccaaaaagtt ggacaaacaa tcgaaatcag
    16441 ttatttgcat aatggtgaag aaaaaacagc ttctggtgat ctgatcgagt taccaacaga
    16501 taaaaaggca ggaataggta tcacgcttac tgaccatacc gagatcgaat cagatacaga
    16561 tgttcgtatt aattctggct ctatcggtgg gccatcagcg ggattgatgt tcactttaga
    16621 aatctatgaa caagtcactg gtaaaaactt gcgacatggt aagcaaattg caggaactgg
    16681 aacgatcaac agtgaaggag aagttggccg aatcggagga atcgataaaa aagtggcaag
    16741 tgcggataaa gctggcgtag agattttctt tgcaccagat gatacgattt cagacgatgt
    16801 taagaaagaa tatcctgaaa taaaaaccaa ctacgaagaa gcaaaagctg ccgctaaaaa
    16861 attgaattcc tcaatgaaaa tcgtaccagt aaaaacagta caagaagcgc ttgactatct
    16921 agagaaaatg aaatgaaact aaataattaa agaggggttg tgacacgagt tctgtcacaa
    16981 cccctctttt tcgtcaaaa
//
LOCUS       DAZKLD010000053        16878 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.053, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000053 DAZKLD010000000
VERSION     DAZKLD010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16878)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16878)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16878
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.053"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(258..1763)
                     /locus_tag="ACG8KR_001439"
     CDS             complement(258..1763)
                     /locus_tag="ACG8KR_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFQ0200816.1"
                     /translation="MIKPKKSEKRELKGPSLTIKWAFASSFFIFVVFTIFAVITYKSS
                     INLIVAKERNNVERTISEVASRLANSDEELTLVNTYRNLTKATNDGDEVVDHTSLVEG
                     NLMKIDSFIAELGQPSMDLYVYDLDENLIFKTHENSRNLIQTTRKVPTIVTLEGKTGF
                     LSIQPIYSKGTREKIGYAQSFYELSSFYDIRNKLLLTLIVLEVVSLILSSILGFFLSS
                     YFLKPLKVLRDTMDTIRKDPQSDIHMPEIDTNDELADLAEIFNEMLDRMRLYIEQQEQ
                     FVEDVSHELRTPVAIIEGHLSLLNRWGKDDPEILEESLSASMQEISRMKSLVQEMLDL
                     SRAEQVDVHYANETTKGKEVTYQVYNNFKILYPDYVITLDDDLTKEVTLQIYRNHFEQ
                     LIIIILDNAVKYSTTRKEVHISISSTLNEFEIAIQDFGEGIPKEDLNKIFNRFYRVDK
                     ARARTKGGNGLGLSIAKQLVENYKGRILAESVVGQGTIFRIFIPIAKAEDK"
     gene            complement(1760..2446)
                     /locus_tag="ACG8KR_001440"
     CDS             complement(1760..2446)
                     /locus_tag="ACG8KR_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFQ0200817.1"
                     /translation="MSNILIIEDEKNLARFVELELKHEGYNAEVHYNGRTGLDAALNN
                     EWDAILLDLMLPELNGLEVCRRVRQVKNTPIIMMTARDSVIDRVSGLDHGADDYIVKP
                     FAIEELLARLRALLRRIDIEGDKNVAKQTTITYRDLTIEKENRVVRRGNEVIELTKRE
                     YELLLTLMENVNVVLARDVLLNKVWGYETEVETNVVDVYIRYLRNKIDVPGEESYIQT
                     VRGTGYVMRS"
     gene            complement(2639..4060)
                     /gene="gndA"
                     /locus_tag="ACG8KR_001441"
     CDS             complement(2639..4060)
                     /gene="gndA"
                     /locus_tag="ACG8KR_001441"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HFQ0200818.1"
                     /translation="MSKQQIGVVGMAVMGKNLALNIESRGYSVALFNRTGAKTTAVVE
                     EHPDKNFKATYTIEEFVESIEKPRRILLMVKAGPATDATIQELLPHLDKGDILIDGGN
                     TFFKDTMRRNEELANSGINFIGTGVSGGEEGALKGPSIMPGGQKEAYALVAPILEQIS
                     AKAEDGAPCVTYIGPNGAGHYVKMVHNGIEYGDMQLIAESYDLMKNILNLSVEEMADI
                     FKEWNQGELDSYLIEITADILTRKDDEGTGKPVVDVILDAAGNKGTGKWTSQSALDLG
                     VPLPLITESVFARYISAYKEERVQASKILSRTNDFEFTGDKKELVEKIREALYFSKIM
                     SYAQGFAQLRVASKEFDWDLPFGEIAKIWRAGCIIRARFLQKITDAYDKNPEIENLLL
                     DDYFVEITKKYQQSVRDVVALAVQAGVPVPTFSSAIAYFDSYRAERLPANIIQAQRDY
                     FGAHTYERVDKEGIFHYSWYHEE"
     gene            complement(4230..4409)
                     /gene="rpmF"
                     /locus_tag="ACG8KR_001442"
     CDS             complement(4230..4409)
                     /gene="rpmF"
                     /locus_tag="ACG8KR_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HFQ0200819.1"
                     /translation="MAVPARRTSKAKKAKRRTHYKLTIKGLNECPNCGEMKKSHHVCA
                     NCGYYDGKDVMSKEA"
     gene            complement(4467..5036)
                     /locus_tag="ACG8KR_001443"
     CDS             complement(4467..5036)
                     /locus_tag="ACG8KR_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HFQ0200820.1"
                     /translation="MKWSLLELRKYQETPLTFHETLDLKAALMKRDNLILDVAPVEVE
                     GLVSVDKKGYIVHYTVQTTLTVPSTRSLEPVELPMDFSVDEVFMTEEQFQSRDEQMSA
                     DEILLIEGPLLNLAESIEDNILLAIPMRVLTKEEEAATELPKGNDWEVVSEEEYQRRK
                     MEEAENKIDPRLAKLSELFDSKSKDDDNK"
     gene            5222..6124
                     /locus_tag="ACG8KR_001444"
     CDS             5222..6124
                     /locus_tag="ACG8KR_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFQ0200821.1"
                     /translation="MNTKDIEYYVQIVKQKNFTKVANYFRVSQPTITYALQRLEKEFD
                     TVLITRDRSHHELIVTPSGKQLFLHAQTILQEISTARAEITQLQKKKLRFGMPPIIGN
                     EYFPKLSSYFIKHELMNQIEITDGGSRDLYGMIRQGRIDLAVLGSTQPIVDQYLETEL
                     LLDKRFMIVVPPTHPLSKQDTVSFKDIADEQFVLLNEHYVHPAAFTKLSKQTHTHPKV
                     VYQSNDLTILKSMVRENVGIGFLAEIAVHPEDHLVCLPLSDEVQPRFLISLAKRTQQI
                     VTPLQEQVVQVIHEFVTAQKNSSS"
     gene            complement(6159..7943)
                     /gene="pyk"
                     /locus_tag="ACG8KR_001445"
     CDS             complement(6159..7943)
                     /gene="pyk"
                     /locus_tag="ACG8KR_001445"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HFQ0200822.1"
                     /translation="MKKTKIVSTLGPASNSVEIISQLIESGANVFRFNFSHGDHEEQL
                     SRMTMVREAVKKTGKDVGILLDTKGAEIRTTVQGTTEADFGRAGYIKFEVGDQTRISM
                     DPEHVGSKEKIAVTYPGLFDDVHVGGHILFDDGLIDMQIIEKDEANRELVVEVKNAGM
                     LGSRKGVNAPGVSISLPGITPKDADDIRFGLDNDIDFIAASFVRKAQDVLDIREILEE
                     KDMTHVQIFPKIESQEGIDNIDEIIKVSDGVMIARGDMGVEIPAELVPMVQKRIIKKC
                     NAAGIPVITATQMLESMQENPRPTRAEASDVANAVFDGTDATMLSGESANGDYPVEAV
                     STMARIDQEAENALSELGTFQLNEFDKTDVTETIGLSVARAAKNLGVKTIVAATESGY
                     TAKMISKYRPDADILAVTFDERTKRGLMLNWGVYPTVAEKPSTTDEMFELAAKKAVEL
                     GFASEGDLILITAGVPVGERGTTNVMKIQMIGSKLLEAQGVGQKSVVANAVIASSAEE
                     AVKKAKDGMVLVVPSTDKEYMPAIEKAAAVIVEEGGLTSHAAVVGIAQDIPVIVGAKG
                     ATTTITEGELITVDSRRGIVYRGETTAI"
     gene            complement(8017..8979)
                     /gene="pfkA"
                     /locus_tag="ACG8KR_001446"
     CDS             complement(8017..8979)
                     /gene="pfkA"
                     /locus_tag="ACG8KR_001446"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010746303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HFQ0200823.1"
                     /translation="MKRIGILTSGGDAPGMNAAVRAVVRKGIYEGLEVYGINYGFAGL
                     VAGDIRRLDVADVGDKIQRGGTFLYSARYPEFATEEGQLKGIEQLKKFGIEGLVVIGG
                     DGSYHGAMALTKRGFPAVGIPGTIDNDIPGTDFTIGFDTAINTVLESIDRIRDTATSH
                     VRTFVIEVMGRNAGDIALWSGVAGGADEIIIPEHDFDMASVAKKIQEGRDRGKKHCLI
                     ILAEGVMGGNEFAEKLSEFGDYHTRVSILGHVVRGGAPSARDRVLASKFGAYAVELLQ
                     EGKGGLCVGIHDNEIVASDIIDTLENNKHKPDLSLYDLNNSLSF"
     gene            complement(9147..12461)
                     /gene="dnaE"
                     /locus_tag="ACG8KR_001447"
     CDS             complement(9147..12461)
                     /gene="dnaE"
                     /locus_tag="ACG8KR_001447"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HFQ0200824.1"
                     /translation="MFFPQLYTITSYSLLQSTIRIREYVQEAKKQGYTSLAITDKDVL
                     YGAPEFYRICVEEGVQPIIGLHLSYTYEEKNYGLLAYAKDEIGYQQLMKLSSRKMIQE
                     KLELAEMTETDHLAFVVPANHSVSEAFNKEDNFFGHWQFLLENVFTRNFYIGLTENTP
                     DQLPEWYQYVIKENYSLCALHPIASLQQEELFALEVMKHIKEGSQIPLENLSASLPLT
                     ANVYLLSEQEMIKRFEKAGLSQAVIGAQRLAESISFEFQFHQKLLPHYPVPNGKQAGE
                     YLRELCYSQVERRITDYTKEYQERLDYELSVIHQMGFDDYFLIVWDVMAFAHRNKIVT
                     GAGRGSAAGSLVAYVLEITDVDPLEYDLLFERFLNPERFTMPDIDLDIPDNRREEVLN
                     YVREKYGQYHMAQIATFGTMAAKMVLRDAARVFGLSQSEANRWSKAIPNQLKITLTQA
                     YKTSHQLVELVQASEKNQLLFKTAVILEGLPRHVSTHAAGVVISDKNLLELVPLQRGS
                     DEAFLTQYTMNEVEAIGLLKMDFLGLRNLSIIDFTLKAVKRVEGYEIRLKEIPLNDAK
                     TLLLFQRGETSGVFQFESAGIRNVLRRLDPENIEDIAAVNALYRPGPMQNIDTFIARK
                     KGKEAIRYPDDSLIPILKNTYGVIVYQEQIMQIASTMAGFTLGQSDILRRAVSKKKKD
                     VLDEEKSHFVSGALQQGYSEKTAATVYEYIEKFANYGFNRSHAFAYSFVGFQMAYLKA
                     HHPGAFFTSLMNSVRHNTSKLKEYIAEARKNKLKLSSPSINQSNSSFELVNKEEIRFG
                     LTAIKGIRRDFVEDILNERKQNGPFQSVDQFLIRIDKRWLKLELLQSLTAVGVFDELS
                     PNRKQLMLDLEGKIQNIVYSGGSLDLLGIMALKEEEVADYTLEERLKLEEEYLGIYLS
                     GHPAEGFDRLRLAKQVQLITDIVPNQPVNLLVMIKEVREIRTKKGELMAFVEGTDSSG
                     EISVTVFPTLYRKYRNLFAVDRVIFVQGRSEISKYNQELQLIGESFADPNQLEAQYPS
                     QTCYLRITEEREDPEIMKQLQVIFQKHPGYIPVVMYHEKTQRKVVLSDAYWVDDSKSI
                     KRQLIYLLGEDNVVFK"
     gene            12692..12877
                     /locus_tag="ACG8KR_001448"
     CDS             12692..12877
                     /locus_tag="ACG8KR_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HFQ0200825.1"
                     /translation="MRYIVTLFWAVVLGQVVGYIGAALTSGTYDFTLTTIISFIAGVI
                     ILLIGAVAPRKETSAHS"
     gene            complement(12939..14135)
                     /locus_tag="ACG8KR_001449"
     CDS             complement(12939..14135)
                     /locus_tag="ACG8KR_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HFQ0200826.1"
                     /translation="MEINWRKNLVIAWIGCFFTGASISLVMPFIPVYVEQLGTPKDQI
                     ELFSGLAISVTAFASAVVAPIWGNLADRKGRKLMMIRAAAGMTLTMGSLAFVPNAYWL
                     LIMRFWNGILSGYIPNATAMIASQAPREKSGWALGTLSTGAIAGNLIGPSMGGALAQW
                     FGMENVFLITGALLMITTVLTIFLVKEDFHPIEKKDLISTKEIFSKMDHLSILIGLFI
                     TTLILQIGITTISPILTLYIRELSGSTENILFVSGLIVSIAGVSAVFSSPKLGKLGDK
                     IGNQKVLIGGLILSFCCYLPMAFVTTPLQLGILRFILGFSTGALMPSVNTLISKITPQ
                     EGVSRIYSYNQMFSNFGQVLGPMLGSTVAHAYNYSAVFIVTSLFVLSNILLSLFNFRK
                     VLRSRL"
     gene            complement(14305..15741)
                     /locus_tag="ACG8KR_001450"
     CDS             complement(14305..15741)
                     /locus_tag="ACG8KR_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HFQ0200827.1"
                     /translation="MEKFFGLKKNNTNVSTEIMAGVTTFFAMSYILFVNPTILSASGM
                     PFQAVFLATIIASIIGTLVMGLFANVPYAQAPGMGLNAFFTFTVVFGLGYSWQQALAM
                     VFICGLINIFITVTNIRKMIIRAIPESLQHAIGGGIGIFVAYVGIKNAGFLSFSADQS
                     AISSSVVEGGKATNVTINGGIVPALANFDNAPILLAVIGLVLTAILVVKNVRGAILIG
                     IVATTVLGIFMGVVDLSSIDWQTNSLGNSFKELGTTFGAAFGSEGMQSLFSDSSKIPQ
                     VLMTIIAFSLSDTFDTIGTFIGTGRRTGIFSKEDEIALEDGRGFKTKMDKALFADAIA
                     TSVGAIFGTSNTTTYVESAAGIGAGGRTGLTSVVVAVLFALSSLFSPLIAIVPNQATA
                     PALILVGVMMMASFADIKWLDMEEALPAFFASIFMGLCYSISYGIAAGFIFYTIVKVI
                     KGKYNQVSVALWIVDILFIINFIILATI"
     gene            16034..16843
                     /locus_tag="ACG8KR_001451"
     CDS             16034..16843
                     /locus_tag="ACG8KR_001451"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFQ0200828.1"
                     /translation="MNKKLIAIDLDGTTLNQDSMITSKTKETLKKAINAGHHVSIATG
                     RPFRMSHQFYQQLELTTPMVNFNGALVHIPNQHWDGERETLINREIAFEILSQKKQLN
                     LDFIAAENRDTFFIDSFDFFDEKIFASSRPGEKNLLSPKNLTTNPTSLLVRTDKRFAG
                     AVSAELTRQFGSYVDVRTWGGPTAILEIVSKGIQKAKGVQEIANYLSIDQKDVIAFGD
                     EHNDLELLDYAGWGVAMANGTDQLKGIANDVTPLSNQEDGLAVYLEKLLKL"
BASE COUNT         4952 a         3539 c         2950 g         5437 t
ORIGIN      
        1 aaataataat atagcaatct ctttataaaa ttgcaataga aaattcccta tttttattta
       61 ttatttttaa acacgaatat tacagcattt tattgttttt tcgttcatct aaaagatact
      121 tacatttaat taacggatgt tattctattt aattgttcgc tagttttttt ataatgacat
      181 ccctgaattt ctcacttgct tgtattttgt accttattct ttgttggata tataaaaaaa
      241 caagtaaagc acatgcttta cttgtcttct gcctttgcga taggaataaa gattctaaaa
      301 attgtccctt gccctactac actttctgct aagatacgtc ctttatagtt ttcaaccagt
      361 tgtttagcga tagacaaccc cagaccattt ccacccttcg ttctagctct tgctttatcc
      421 actcgataaa aacgattgaa gatcttatta aggtcttctt tgggtattcc ttcgccaaag
      481 tcctgaatcg cgatctcaaa ttcattcaga gtggaagaaa tagagatatg gacttctttt
      541 cgtgtagttg aatactttac tgcgttatcc aatatgataa tgattaattg ttcaaagtga
      601 ttacgataaa tttgcagggt cacttctttt gtcaaatcat catctaaagt gatcacatag
      661 tctggatata atatcttgaa attattataa acttgatagg tgacttcttt tcctttagtt
      721 gtttcattcg cataatgaac atctacttgt tctgctcttg ataagtctag catctcttga
      781 actaagcttt tcatacggct gatttcttgc atgctggcgc ttaatgattc ttctaatatc
      841 tcaggatcat ctttccccca acgatttagc agactaagat gcccttcaat aattgcaaca
      901 ggtgtcctta actcatgaga tacgtcttcc acgaattgct cttgttgttc aatatacaaa
      961 cgcatacggt caagcatctc attgaatatt tcagctaaat ctgccaattc atcatttgtg
     1021 tcgatttcag gcatgtggat atctgattgc ggatctttac gtatagtatc cattgtatct
     1081 ctgagtacct tgagtggttt taagaaataa gaagataaaa agaaacctaa aatactactt
     1141 aagatcaaag aaacaacttc taaaacaata agagtcaata atagcttatt tcgaatatca
     1201 tagaaagaag ataattcata gaagctttgc gcatagccga tcttttctct tgtaccttta
     1261 gaatatatag gctggataga taaaaagcca gtttttcctt ctagtgtaac gatcgttgga
     1321 actttacgtg tagtctgaat caggtttcga ctgttttcat gggttttaaa aatcaagttc
     1381 tcgtccaaat cataaacata aagatccatt gatggctgcc ctagctcagc tataaaggaa
     1441 tcgatcttca ttaagtttcc ttcgaccagt gaagtatgat ctacgacctc atctccgtca
     1501 tttgtcgctt tcgtcagatt acgatacgta tttactaatg tcagttcttc atccgagtta
     1561 gccaaacgag aagctacttc tgaaatcgta cgctctacat tgtttctttc ttttgccaca
     1621 atcagattga tagatgattt ataggtaatg acagcgaaaa ttgtaaacac aacgaatatg
     1681 aagaaagaac tcgcaaaggc ccacttgatt gtcaaggagg gccctttcag ttctcttttt
     1741 tccgattttt tcggctttat cacgagcgca tcacataacc tgttccgcgt acagtttgga
     1801 tatagctttc ctccccggga acgtcaattt tgttgcgaag ataacgaata tatacgtcca
     1861 ctacgtttgt ttcaacttct gtttcgtatc cccacacttt atttagtaaa acgtcacgtg
     1921 ctaataccac gttcacgttt tccattaaag taagcagcaa ctcgtattct ctttttgtta
     1981 gttcaattac ttcatttccg cgacgtacga cacggttttc tttttcgata gtcaaatcgc
     2041 gataagtaat agtagtttgt ttagccacat ttttatcgcc ttcaatatcg attcgacgca
     2101 acaatgcacg aagacgtgct aataattctt cgatagcaaa tggtttgacg atgtagtcat
     2161 ctgctccgtg gtcaagtcca gatacacgat cgatcacaga atctcttgca gtcatcataa
     2221 tgattggtgt atttttgact tggcgtacac gtctgcagac ttctaaaccg ttcaattctg
     2281 ggagcatcag atccaacaat atggcatccc actcattatt taatgccgca tctaaaccag
     2341 ttcggccgtt gtagtggact tctgcgttat acccttcatg cttcaactct agctcgacaa
     2401 atcgtgccaa gttcttttca tcttcaatga ttagaatatt gctcatttat tattttatcc
     2461 tttctattct actggctttt taaaaactct atcattatac cgataatatg cttaaaaatc
     2521 tatagtcatt ttattatgga tagcaatttt aaaaaagagc tgaagtctac tcataacaat
     2581 cgacttcagc tctttcgtcc tctttttatt ttctcatttc ctaacagccg aaagactatt
     2641 attcttcatg gtaccatgag taatggaaga ttccttcttt gtcgacacgc tcataggtat
     2701 gtgcgccaaa gtaatcgcgt tgggcttgga ttatatttgc aggcagacgt tctgctctgt
     2761 atgaatcgaa gtaagcgatg gctgatgaga aagtcggtac aggtacgcca gcttggacag
     2821 ccaaggcaac gacgtctctg actgattgct gatatttttt cgtgatttca acgaagtaat
     2881 catctagcaa taaattttcg atctcaggat ttttgtcata ggcatccgta attttttgca
     2941 agaatcgagc tcggatgata catccagcgc gccagatttt tgcgatttca ccaaatggca
     3001 agtcccaatc aaattctttt gaagcgacac gtaattgagc aaatccttgg gcataactca
     3061 tgattttact gaaatacaac gcttctcgga ttttttcgac caattctttt ttgtctcccg
     3121 tgaactcaaa atcgttcgta cgagacaaaa tcttgcttgc ttgtacgcgt tcttctttat
     3181 aagcagaaat atagcgggca aatacagatt cagtgatcaa tggaagtggc acacctaagt
     3241 caagcgcgct ttggcttgtc catttacctg ttcctttatt cccagctgca tcaaggatca
     3301 catctactac tggtttacct gttccttcat cgtctttacg tgtcagaatg tcagcagtga
     3361 tttctatcaa atagctgtct aattcgcctt gattccattc tttaaagata tcagccattt
     3421 cttctactga aaggtttaaa atattcttca ttaagtcata agattcagca atcagttgca
     3481 tatctccata ctcaataccg ttgtgtacca ttttcacata atgtccggct ccatttgggc
     3541 caatatatgt gacacatggt gctccgtctt ctgcttttgc agaaatttgc tctaagattg
     3601 gtgcaactag cgcataggct tctttttgtc caccaggcat gattgaaggt ccttttaaag
     3661 ctccttcttc accaccggaa acgcctgtac cgataaagtt gatgccagag ttggctaact
     3721 cttcattacg acgcatcgta tctttaaaga atgtatttcc tccgtcaatc aaaatatcgc
     3781 ctttatcaag gtgaggaagt aactcttgga ttgttgcatc tgttgccggt cctgctttga
     3841 ccattagcaa gatccgacgt ggtttctcga tagattctac aaattcttcg attgtatatg
     3901 ttgctttgaa atttttatct gggtgttctt ctactacagc ggttgtttta gcccctgtac
     3961 gattgaataa agccactgaa tagccgcggc tttctatgtt caaggctaga tttttaccca
     4021 taacggccat accgacaacg ccgatttgtt gttttgacat atgaactcct cctacatacg
     4081 ggatttctcc ctaatctctt acattatagc ttaacacggc tacattgcaa actaaagaac
     4141 aacatatttt ttatttaccg actacaaaaa aataaacctc aaggctccat ggttagcctt
     4201 gaggttttac attttctaat taaaatgtat taagcttctt ttgacattac gtccttacca
     4261 tcatagtaac cacaatttgc acatacgtga tggctttttt tcatttcacc acagtttgga
     4321 cattcgttta accctttgat tgtcaatttg tagtgtgtac gacgttttgc ttttttagct
     4381 tttgatgttc ttctagctgg tactgccatt ttgctacacc tccttgtaaa gtcttgtatc
     4441 taaattagac acaatatatt ccaatcttac ttgttatcgt cgtcttttga tttagaatca
     4501 aaaagttcag atagttttgc aagacgagga tcaattttat tctcggcttc ttccattttt
     4561 ctgcgctggt attcttcttc ggaaacaact tcccaatcat tgccttttgg aagttctgtt
     4621 gctgcttcct cttcttttgt caatacccgc attggaatcg ccaataagat gttgtcttca
     4681 atcgactctg ctaaattcaa taatggacct tcaattagca aaatctcatc tgcagacatt
     4741 tgttcatctc tactttggaa ttgttcttct gtcatgaata cttcgtctac agaaaaatcc
     4801 atgggcagtt caacaggttc tagtgaacgt gttgaaggaa ctgtcaaagt cgtctgcaca
     4861 gtgtaatgaa cgatatagcc tttcttatca actgagacca aaccttcgac ttccactggc
     4921 gcaacatcaa gtattaaatt gtctcgcttc atgagagcgg ctttcaagtc gagcgtctca
     4981 tgaaaagtta acggtgtctc ctggtatttt ctcaattcca ataaagacca tttcattttt
     5041 catcactcct aagcaacaaa agcaattata cagggcaaaa agcatgttgt caatgaaatt
     5101 ttcttgacac cttgcagttc ataaaaaaac agaaaaatct ttattagaaa gcctctgttt
     5161 gctatcatcc tcagaatcat ttacaataat cattattcca ttaaaggagc ggatgatctt
     5221 catgaacacg aaagatatcg aatactatgt ccaaatcgtc aaacaaaaaa attttacgaa
     5281 agtggctaat tatttccgtg tgagccaacc tactattaca tatgcattac aacgtcttga
     5341 aaaagagttt gacaccgtac ttattacaag agatcgatcg catcacgaat tgatcgtcac
     5401 gccttctgga aagcagctgt ttctccatgc acaaaccatt ctccaagaga tttctactgc
     5461 aagagctgaa atcactcagt tgcaaaagaa aaagcttcgt tttggcatgc ctcctattat
     5521 cggcaatgaa tactttccta aactgtcaag ttactttatc aagcatgaat tgatgaatca
     5581 aattgagatt accgatggag gctctcggga cttatacggg atgatccgac aagggaggat
     5641 cgatttagct gtattaggct caacacagcc aatcgtggat caatatttag aaacagagtt
     5701 acttttggac aaacgtttca tgatcgtcgt gccacctaca catcctcttt ccaagcagga
     5761 tactgtctct ttcaaagata tagcagatga acagtttgta ctattgaacg aacattatgt
     5821 ccaccccgca gcattcacta agctttctaa acaaacacat actcatccaa aagttgttta
     5881 tcaaagcaat gacttgacga ttttaaagag tatggtccga gaaaatgtgg gtatcggttt
     5941 tttggcagag attgctgtcc atcctgaaga tcatttagtt tgcctccctc tttcagatga
     6001 agttcagcct cgcttcttga tttcattggc aaaacgaaca cagcagatcg tcacgcctct
     6061 tcaagaacaa gtagtccaag ttatccatga attcgttaca gcacaaaaaa acagctcttc
     6121 gtaaaagaag agctgttttt attttttatt aagctaaatt agatagctgt tgtttcgcca
     6181 cggtaaacga tgccgcgacg tgaatcaacg gtaatcaatt caccttctgt aattgttgtt
     6241 gttgcgcctt tagcaccaac gattacagga atatcttgtg caattcctac aactgcagcg
     6301 tgagaagtca atccgccttc ttcaacgata acagcagctg ctttttcgat tgcaggcata
     6361 tattctttgt cagttgatgg tacaactaga accattccgt cttttgcttt ttttacagct
     6421 tcttcagcag atgaagcaat aactgcattt gcaacgacag atttttgtcc aacgccttga
     6481 gcttctaata atttagaacc aatcatttga attttcatca cgttagttgt tccgcgttca
     6541 ccaactggta caccagcagt gatcaagatt aaatcgcctt ctgatgcgaa acctaattca
     6601 acagcttttt tagctgctag ttcaaacatt tcatcagttg ttgatggttt ttcagcaact
     6661 gttgggtaaa cgccccagtt taacatcaaa ccacgttttg tgcgttcgtc aaaagtaaca
     6721 gccaagatat ctgcatctgg acgatatttt gaaatcattt ttgcagtgta tcctgattca
     6781 gttgcagcaa cgattgtttt cacgcctaga ttttttgcag cacgagctac agataaaccg
     6841 attgtttcag ttacatctgt tttatcgaat tcgttcaatt ggaatgtacc taattctgat
     6901 aatgcatttt cagcttcttg gtcgatacgt gccattgttg aaacagcttc aactgggtag
     6961 tccccgtttg cagattcacc agatagcatt gttgcatctg tgccatcaaa tacagcgtta
     7021 gcaacgtcag aagcttccgc acgtgttgga cgtgggtttt cttgcattga ttctaacatt
     7081 tgtgtagcag tgataactgg gataccagca gcattacatt ttttgataat gcgtttttga
     7141 accattggta ctagttcagc tgggatttca acacccatat ctccgcgggc aatcataaca
     7201 ccgtcagaaa ctttgatgat ttcgtcaatg ttgtcgatac cttcttgaga ttcgatttta
     7261 gggaagattt gaacatgagt catgtctttt tcttctaaga tttcgcggat atctagaacg
     7321 tcttgtgctt tacgtacaaa acttgcagcg ataaagtcga tatcgttgtc taaaccaaaa
     7381 cgaatatcgt cagcatcttt tggagtaata cctggcaaac taatagaaac gcctggtgca
     7441 tttacccctt tacgtgatcc tagcatacct gcatttttaa cttctacgac taattcacgg
     7501 tttgcttcgt ctttttcaat gatttgcata tcgatcaaac catcatcaaa taaaatgtgt
     7561 ccgccaacgt gaacatcgtc aaataatcct gggtaagtaa ccgcgatttt ttcttttgaa
     7621 cctacatgtt caggatccat agagatgcgt gtttgatctc ctacttcaaa tttgatgtat
     7681 cctgcacgac caaaatcagc ttctgttgtg ccttgaacag ttgtacggat ttcagctcct
     7741 tttgtatcta aaagaatacc gacatctttt cctgtctttt taactgcttc acggaccata
     7801 gtcatacgtg ataattgttc ttcatggtca ccatgagaaa aattaaagcg gaagacattt
     7861 gccccagatt caatcaattg agaaataatt tctactgagt tacttgctgg tcctaatgta
     7921 ctaacgattt tggttttttt catttaaaaa actctcccat tcaattttgt ttatttaaag
     7981 ctgtacacag cttaaatagc ttggtttctt tcactcttaa aatgaaagtg aattatttag
     8041 atcatatagt gataagtctg gtttatgttt gttgttttca agtgtatcaa tgatgtcaga
     8101 cgcaacgatt tcgttgtcat ggataccgac acataatcca ccttttcctt cttgaagcaa
     8161 ttcgacagcg taagcaccga atttacttgc caacacgcgg tcgcgtgcgc ttggtgctcc
     8221 accacggaca acgtgtccca agatagatac acgtgtatga tagtcaccaa attctgaaag
     8281 tttctcagcg aattcatttc cgcccattac accttcagcc aagatgatca agcaatgttt
     8341 cttcccgcga tcgcggcctt cttggatttt tttagcaact gaagccatat caaaatcatg
     8401 ttctgggatg atgatttcat ccgctccgcc agccacacct gaccataaag cgatgtctcc
     8461 tgcattgcgt cccattactt cgataacgaa tgtacgaaca tgagaagtcg ctgtgtcacg
     8521 aatacggtca attgattcta gtactgtatt gattgctgta tcaaaaccga ttgtgaaatc
     8581 agtcccagga atatcattat cgattgttcc aggaatacct actgctggga aaccgcgttt
     8641 tgtcaaagcc attgcgccat gataagagcc gtctcctccg ataaccacta gaccttcgat
     8701 tccgaatttt ttcaattgtt cgattccttt taattgacct tcttcagttg caaattctgg
     8761 ataacgtgca gaatacaaga atgtaccacc acgttggatc ttgtcaccta catctgccac
     8821 atcaagacga cgaatatctc cagcaactaa accggcaaaa ccataattga tcccgtatac
     8881 ttccagtcct tcgtaaatac ctttacgaac aacggcacga acagctgcat tcattccagg
     8941 tgcgtctccg ccactggtca aaattccgat gcgtttcata ataaacattc acctcattaa
     9001 aatactttat cctaagtatg acttaggttt acaaccattt ttgcgttaac cacgactaac
     9061 attctaacat tatttttctc acttgtcttg atttacggat aaaaaaatga aaaaaaatga
     9121 aagcaagtag tattttttac cgtaagttat ttgaaaacca cattatcttc gccaagcaga
     9181 taaataagtt gccgctttat actcttgctg tcatccaccc aataagcatc tgacagaact
     9241 acttttcgtt gcgttttttc gtgatacata actactggta tatatcctgg gtgtttctga
     9301 aaaatcactt gtaattgttt cattatctcg ggatcttccc gttcttctgt aatacgcaaa
     9361 tagcaagttt gtgaaggata ctgggcttct aactggttag gatcagcaaa gctttctcca
     9421 attagttgca gttcctgatt atatttgctg atttcgctcc gcccttgaac aaagatcact
     9481 cgatcaacgg caaacaaatt acgatattta cgataaagag ttggaaaaac tgtgacagaa
     9541 atttctccac tactgtcggt tccttcaaca aaggccatca gttctccttt ttttgtccga
     9601 atttctctaa cttccttgat catcactaat aaattgactg gttgattagg gacgatatct
     9661 gtaatcagct gaacttgttt agctaaacga agacgatcaa acccttctgc aggatgacct
     9721 gacaaataaa tacctaagta ctcctcttcc agcttcaagc gttcttctag tgtatagtca
     9781 gcgacttctt cttcttttaa tgccatgatt cccagcaagt caagacttcc accactgtaa
     9841 acgatatttt ggatttttcc ttcgagatca agcatcaatt gtttcctgtt aggagataat
     9901 tcatcaaaaa ctcctactgc tgtcagtgat tgcagtaact ctaatttcag ccaacgctta
     9961 tcgatccgta tgaggaattg atccactgac tggaacgggc cattttgttt tctttcgttt
    10021 aatatatctt ctacaaaatc acgccgaata ccttttatcg ctgtcagtcc gaatctgatt
    10081 tcttccttat tgaccaactc aaaactggaa ttactttgat taatggacgg cgaggaaagt
    10141 ttgagtttat ttttacgtgc ttctgcaata tattctttca attttgatgt gttatgccga
    10201 acagaattca tcaggctggt aaagaaagca cctggatgat gggcttttag atacgccatt
    10261 tggaatccca cgaatgaata agcaaatgca tgtgaccgat tgaatccata gtttgcaaat
    10321 ttttcgatat attcatatac ggtcgctgct gttttttccg aatatccctg ttgtaatgct
    10381 cctgatacaa aatgcgactt ttcttcatct aatacgtctt ttttcttttt actcactgcc
    10441 cgtcgcagaa tatctgactg ccctaatgta aatccagcca tagtcgaggc gatctgcatg
    10501 atttgttcct gatagacgat cactccatat gtgtttttca aaatcgggat cagtgaatca
    10561 tctgggtagc gaatggcttc ttttcctttc ttcctggcaa tgaaggtatc tatattttgc
    10621 atagggcctg gtcgatacaa tgcattgact gcggcaatat cttcgatgtt ttctggatct
    10681 aatcttcgta aaacgttccg gataccagca gattcaaatt gaaatactcc tgaggtttcg
    10741 cctcgttgaa acaagagcag cgtttttgca tcatttagcg ggatctcttt taatcggatc
    10801 tcataccctt ctactcgctt aacagctttc aaagtaaaat caataatcga taagttgcgt
    10861 agtcctaaaa agtccatttt caaaagaccg attgcttcta cctcattcat cgtatactgg
    10921 gtcagaaaag cttcatcaga accgcgttgg agcggaacta gttcaagtag attcttatcg
    10981 ctgataacta cacctgctgc gtgagtcgaa acatgtcttg gcaatccttc taaaatcact
    11041 gcagttttga ataaaagttg gtttttttcg cttgcctgaa ctaattctac taattgatgc
    11101 gaagttttat aagcttgggt caatgtgatt ttcaattgat tgggtattgc ttttgaccag
    11161 cggtttgctt cactttgcga taaaccaaac actctagctg catctctaag aaccattttt
    11221 gctgccattg tgccaaatgt tgcgatttgt gccatatgat attgtccata cttttctcga
    11281 acataattca atacttcttc acgtcgattg tcaggaatat ccaaatcgat atctggcatc
    11341 gtaaagcgtt cggggtttaa aaagcgttcg aacagcaaat catattctaa tggatccaca
    11401 tctgtgattt ctaacacata ggctactaat gaacccgcag cagaaccacg acctgcaccg
    11461 gtaacgattt tgtttctatg agcaaaagcc attacatccc atacgatcaa gaaataatca
    11521 tcgaatccca tttgatggat gacagataac tcgtagtcca aacgttcttg gtattccttt
    11581 gtatagtcag tgattcggcg ttccacttgc gaatagcata actctcttaa atattctcca
    11641 gcttgttttc cattaggaac tggataatgc ggaagtaatt tctgatggaa ctggaattca
    11701 aatgaaatcg attcagctaa acgctgggcg cctattacag cttgggaaag acccgctttt
    11761 tcgaatcgtt tgatcatttc ttgttctgaa agaagataga catttgctgt tagtggtaaa
    11821 gatgcagata aattttctaa aggtatttgg cttccctctt tgatatgttt catcacttca
    11881 agtgcaaaaa gttcttcttg ttgtaaacta gcgatgggat gtaatgcaca aagcgaataa
    11941 ttttctttta tcacatactg ataccattca ggcaattgat caggcgtgtt ttctgtcaag
    12001 ccgatataaa aatttctagt aaaaacattt tcaagtaaaa actgccaatg accgaaaaag
    12061 ttatcctctt tattgaaagc ctcagaaaca gaatgatttg ccggaacaac aaacgctaga
    12121 tggtcggttt ctgtcatttc tgcaagttcc aatttttctt ggatcatttt tcggctggaa
    12181 agtttcatca gctgctgata acctatttca tcttttgcgt acgcaagcaa gccataattt
    12241 ttttcttcat aagtataact aagatgtaat ccaatgattg gctgtactcc ttcttctaca
    12301 caaatacgat agaattcagg tgcaccgtat aagacatctt tatccgttat ggctaatgaa
    12361 gtgtagcctt gtttttttgc ttcttgtaca tattctcgaa tacgtatcgt actttgtaaa
    12421 agagaataag aagtaattgt ataaagttgt gggaaaaaca tcttgcattc cgcctttttt
    12481 ttcgttaaaa ttcactagcg ttaaaaggtt tgatcctttt actttcatta agatagcagg
    12541 cgctgaagtt gattattcca cttcatttgc ttgcttgtaa tctgtaggaa tgagttcttt
    12601 cgcaagaact caaattttat tgagatttgg tctcgtttct ttacattcaa agaaaatctg
    12661 gtaaaattaa gatagaaaag aggtgttctt tatgcgatat atcgttacat tattctgggc
    12721 tgtcgttcta ggtcaagtag tcggttatat tggtgctgca ttaacaagcg gaacatatga
    12781 ttttactttg acaacaatca tttcattcat tgcgggtgtc attatccttt tgattggtgc
    12841 agttgcgcca agaaaagaaa cctctgccca ttcgtaaaaa aacagccaag agcttgagat
    12901 ttctcaagct cttttttatt ttctactcct caccaccatt ataatctgct gcgaagaact
    12961 ttacgaaagt tgaataaaga aagaagaata tttgacagaa caaacaaact tgtcacaata
    13021 aaaacagcac tgtaattata agcatgcgcg actgtcgatc ctagcatggg acctaaaact
    13081 tgtccaaagt tgctaaacat ctgattgtag ctatagattc ggctgactcc ttcttgaggc
    13141 gtaatcttac tgatcagtgt attgacagaa ggcatcaaag cgccagttga aaagcctaaa
    13201 ataaagcgaa gtatacctaa ttggagcggt gtagtgacga atgccatagg taaataacag
    13261 caaaatgata aaatgagtcc tccaattaga actttttgat tcccaatctt atcccctagt
    13321 tttcctagtt tcggtgaaga aaagacagcg gaaacaccag ctatcgaaac aatcaagccg
    13381 ctgacaaaaa ggatattttc tgtacttccg ctcaattcac gaatatataa ggtcaaaata
    13441 gggctgatcg tagtgattcc tatctgtaaa atcaaggtcg taatgaagag accgatcaaa
    13501 atagagagat gatccatttt tgaaaaaatt tctttggtgc ttatcagatc ttttttctcg
    13561 attggatgga aatcttcttt gactaagaaa atggtcaata ccgttgtgat catcaacaaa
    13621 gcccctgtga tcaagaatac attttccata ccaaaccact gagccagtgc accgcccatt
    13681 gaaggtccga tcagatttcc agcaatcgct ccggtagaca atgttcctaa cgcccatccg
    13741 ctcttttctc taggtgcctg tgaagcaatc atcgctgtgg catttggaat atatccagac
    13801 aaaatcccat tccaaaaccg catgatcaaa agccaatacg cgtttggtac aaaagccaaa
    13861 gatcccattg ttaatgtcat ccctgctgct gcacggatca tcatgagttt tcggcctttt
    13921 cgatcagcta gatttcccca gattggtgca accacggcag atgcaaaagc agtcacagag
    13981 attgctagtc ccgaaaacaa ttcgatttga tcttttggtg ttcctagttg ctcaacataa
    14041 actggtatga atggcataac taaactaata cttgcaccag tgaaaaaaca gccaatccac
    14101 gcaatcacta aattttttct ccaattaatc tccatctatc atccctcttt cgtctctttt
    14161 atcataccta ttttcgaata aacaagcaac gatctcgcca gtaaaaagag aaaaatcccg
    14221 caaaggtcat tttatatgta acgttctttt tatgtaaaac aaaaagagat cagaacaaat
    14281 actgttccaa tcccttctct ttagttaaat tgttgctaga atgatgaagt tgataatgaa
    14341 taaaatatca acgatccaca aagcaacaga aacttgattg tattttcctt tgattacttt
    14401 cacaattgta tagaaaataa atcctgctgc aatcccatag gaaatgctat agcacaatcc
    14461 cataaagatc gatgcaaaga aggccggtaa agcttcttcc atatctagcc atttgatgtc
    14521 tgcaaaagaa gccatcatca ttactccaac taaaatcaat gctggcgcag ttgcttgatt
    14581 tggtacaatg gcgatcagtg gagagaataa actacttaat gcgaatagca ctgccactac
    14641 tactgaagtc aaaccagtac gtccaccggc cccgatccca gctgcagatt cgacataagt
    14701 tgttgtattc gatgtaccaa agatagcccc cacagaagta gcgatcgcat cggcaaatag
    14761 tgctttatcc atttttgttt tgaacccacg accatcttcc aacgcgatct cgtcttcttt
    14821 tgagaaaata cctgtgcggc gtcctgtacc gataaatgtt ccgatcgtat cgaaagtatc
    14881 agataaactg aatgcgatga tcgtcatcaa tacttgagga atcttggaag aatcactgaa
    14941 taaagattgc atgccttcag aaccaaatgc tgcaccgaaa gttgttccta attctttaaa
    15001 tgaattacct aacgaattcg tttgccaatc aatggaactt aaatcaacaa cacccataaa
    15061 aataccaagg actgtggtcg ctacgatacc gataagaata gccccacgga cattttttac
    15121 aactaaaatg gctgtcagta ccaaaccaat cacagccaat aagataggag cgttgtcaaa
    15181 gttggctaac gcagggacga ttccaccatt tatcgtaaca tttgtcgctt ttccgccttc
    15241 aacgacagag ctcgaaatag ctgactggtc tgctgaaaaa gataagaatc ctgcattttt
    15301 gattccgaca tacgcaacga aaataccgat cccgccacca atcgcatgtt gaagactttc
    15361 gggaatcgcc cgaataatca tttttcgaat atttgtgaca gtaataaaaa tattgattaa
    15421 tccgcaaata aacaccattg ccaaggcttg ttgccaagaa taaccaagcc caaaaacgac
    15481 cgtaaaagtg aaaaatgcgt tcaatcccat accaggtgcc tgagcatatg gtacatttgc
    15541 gaacaatccc attaccagcg taccaataat cgatgcaatg attgtcgcta gaaatactgc
    15601 ttggaagggc atacctgatg ctgacaaaat cgtcggattt acgaataaga tataactcat
    15661 ggcaaagaat gtcgttactc ccgccattat ttcagttgat acatttgtat tgtttttctt
    15721 taaaccgaaa aacttttcca taaaaagttc cactccctga atttttttct gatggaaaac
    15781 tataataata aaaaataagt aaaatagggc aaatccatcg aacaatttca aattatatag
    15841 acgtttcatt cgcttttcaa cgttttttta acaaaaaaag cgaaaataat acttaacgtt
    15901 cgtgtttagc ttatgtttag ctatttatat agccataact gtagtttttg ttaggaatag
    15961 ctgattgctc ttgttaatta cctaaaatat gctagactaa aacaaatgct agttgaggaa
    16021 ggaaggaccc ttattgaata aaaaattaat tgccatcgat ctggacggaa ctaccctcaa
    16081 tcaagattcg atgattacat ctaaaacaaa agagactcta aaaaaagcaa tcaatgccgg
    16141 tcatcatgtc agtattgcta caggacgccc attccgaatg agccatcagt tttaccaaca
    16201 attagaatta acaacaccga tggttaattt taacggcgca ttagtacaca tccctaatca
    16261 gcactgggat ggtgaaagag aaacgttgat caatcgtgaa atcgcttttg aaatcttatc
    16321 gcaaaaaaag cagctaaatt tagattttat cgctgctgaa aatcgtgata ctttttttat
    16381 tgacagtttt gatttctttg atgaaaaaat ttttgcttct tctcgtccag gagagaagaa
    16441 tttgctttct cctaaaaatt tgacgacaaa tcctacatct ttgcttgtcc gaacagacaa
    16501 acgttttgca ggagctgtat ctgctgaatt aacacgtcaa tttggcagtt atgtcgatgt
    16561 tcggacatgg ggtggaccta ctgccatctt agagattgtt tcaaaaggaa tccaaaaagc
    16621 aaaaggcgtg caagagattg ctaactacct gtctatcgat caaaaagacg tcattgcctt
    16681 tggtgacgag cataatgatt tggaattgtt ggattatgca ggttggggag tagctatggc
    16741 taacggtact gaccaactca aaggcattgc aaatgatgtc actcctttga gcaatcagga
    16801 agacggttta gctgtttatc tggaaaaact acttaaacta taatagagaa ttagattcca
    16861 caaaaaagag gttgtgac
//
LOCUS       DAZKLD010000054        16795 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.054, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000054 DAZKLD010000000
VERSION     DAZKLD010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16795)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16795)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16795
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.054"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..151)
                     /locus_tag="ACG8KR_001452"
     CDS             complement(<1..151)
                     /locus_tag="ACG8KR_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFQ0200829.1"
                     /translation="MKKNAKQIVHELYNDISISKDPKYSDILEVLQKVYLKLEKQKYE
                     LDPSPL"
     gene            complement(153..350)
                     /locus_tag="ACG8KR_001453"
     CDS             complement(153..350)
                     /locus_tag="ACG8KR_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II bacteriocin"
                     /protein_id="HFQ0200830.1"
                     /translation="MKHLKILSIKETQLIYGGTTHSGKYYGNGVYCTKNKCTVDWAKA
                     TTCIAGMSIGGFLGGAIPGKC"
     gene            complement(744..2471)
                     /locus_tag="ACG8KR_001454"
     CDS             complement(744..2471)
                     /locus_tag="ACG8KR_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate binding
                     protein"
                     /protein_id="HFQ0200831.1"
                     /translation="MNNLLNYQLPVANWVENITEWFTTTFSGLFSFLQTIGQAVMSGI
                     TNLLLVIPAPLFILLLTVAAFFISKKRPGLTLFTLIGLWFIYNQGLWNDLMNTVTLVL
                     LSSVISIIIGVPLGILMAKSSKAQSIIKPILDFMQTMPGFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLGIRQVPKELVEASDSFGSTSRQKLFKLELPLAKSTIMAGIN
                     QTTMLSLSMVVIASMIGAPGLGRGVLSALQRAQVGNGFVNGVALVILAIIIDRFTQHL
                     NQPNNKKAAGATTQKSKKRQGLIIGAVVVVILGAIGIGSFSSAKETKRINLSYVEWDT
                     EVASTNVVGEVLKQMGYDVTMTPLDNSIMWKSVSNGESDAMVSAWLPKTHGSQYAQYK
                     DQVEDLGANLTGAKVGLAVPAYMDVNSIDELTDQAGKKIIGIEPGAGVVTAAENTIQK
                     YDNLKDWKVETSSSGAMTVALGQAIKKHEPIVVTGWTPHWMFAKYDLKYLEDPENGMG
                     SEEQIHTMVRKGLKEDQPEAYKVLDNFHWSEKDMEKVMLEINNGKDPQQAAKDWIKEN
                     QELVESWKK"
     gene            complement(2464..3657)
                     /locus_tag="ACG8KR_001455"
     CDS             complement(2464..3657)
                     /locus_tag="ACG8KR_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HFQ0200832.1"
                     /translation="MVKVEVKHLTKIFGKKTQAALDMMNDHQPKTEILKKTGATVGVY
                     DVNFDVKEGEIFVIMGLSGSGKSTLIRLLNRLIEPTSGSIYIDGEDVSKLSKEELREV
                     RRHKINMVFQNFGLFPHRTILENTEYGLEVRGVPKEERQEKAEKALENSSLLSFKDQY
                     PSQLSGGMQQRVGLARALANDPEILLMDEAFSALDPLIRREMQDELLDLQANVQKTII
                     FITHDLNEALRIGDRIALMKDGEIMQIGTGEEILTNPANDYVREFVEEVDRSKVLTAQ
                     NIMVPALTTNIESDGPNVALTRMRNEEVSMLMAVDRKRHLKGIITADQALEARKQKRP
                     LIDFLDENVTVIGKDMVVSDIFNIIYDSPTPLAVVEDGKLKGVVIRGSVIEALAETSE
                     VSEHE"
     gene            complement(3844..4488)
                     /locus_tag="ACG8KR_001456"
     CDS             complement(3844..4488)
                     /locus_tag="ACG8KR_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFQ0200833.1"
                     /translation="MSGKKKIVRPRYQQIAVDLAERIVENRYKVGEKIHARSTLASTY
                     NASPETTRKAINVLVDLGIMEVRHGSGAFVASKEKAKDFLLQYQDVQSIQDTKAEIMA
                     SVEKQQDELNHFSQLLDQLVNQTKRIHKMNPLLPFELSLSEKAMHLDRSISELNIWQA
                     TGATVIAISHNEELLVSPGPYAKFTAGDIVHFIGNEYTLQRMTNFFYPEHQLAE"
     gene            complement(4582..4716)
                     /locus_tag="ACG8KR_001457"
     CDS             complement(4582..4716)
                     /locus_tag="ACG8KR_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HFQ0200834.1"
                     /translation="MATFLLSVLIFGSAGAIVYTRLKSGKNCDDCQTACPVKKEQIKR
                     "
     gene            complement(4718..6850)
                     /gene="feoB"
                     /locus_tag="ACG8KR_001458"
     CDS             complement(4718..6850)
                     /gene="feoB"
                     /locus_tag="ACG8KR_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HFQ0200835.1"
                     /translation="MNIALVGNPNSGKTSLFNLLTGSNQYVGNWPGVTVEKKEGSLKK
                     NPNIHIQDLPGIYSLSPYTPEEVIARDYLVQEQPELIINIIDATNLERNLYLTTQLLD
                     LDIPMIIGLNMMDVIKKEGKKINLEKLSYGLGVPVLPISVLKKQGIEKLIEKIQESAK
                     KRPSIPVPCTYDPRLEAALHEIEDVLGEQSGKKRWYAMKYFERDQKVREDYEIAVSQQ
                     KEIEQLIQLTEKLLDDDSETILVNERYEFITQLCTLCVVSNDSFQLSMSDKIDQIATN
                     RWLALPIFAFVMWLIYYLAIQTVGTMGTDWINDTLFGTWLPEHVGRLLAEWQVAGWMQ
                     ELILNGIIAGVGAVLGFLPQLIILFLCLSFLEDCGYMARIAFVMDRLFRKFGLSGKSF
                     IPMLIATGCGVPGVMASRTIENEQDRRLTIMVTTFMPCSAKLPIIALVAGAFFPHQSW
                     VAPSAYFLGMTAIIFSGISLKKTRLFAGDTAPFIMELPAYHFPQWSAVLRQTYDRSKS
                     FVKKAGTIIFVSSIVIWFSSSYNFYAQPVSEDQSILAFFGRILAVVFQPLGWGNWQGT
                     VAAITGLVAKENIIGTFGILFGHAEVSENGREIWQVLQHTYTPAAAYSLLAFNLLCAP
                     CFAAIGAIHREMNAKKWTWIAIGYQCGLAYLVSFVIYQLGHLLEGGQVALGTYLAILL
                     MIGLVYVLFRQPKSKNQFYTILSLEGEE"
     gene            complement(6847..7320)
                     /locus_tag="ACG8KR_001459"
     CDS             complement(6847..7320)
                     /locus_tag="ACG8KR_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HFQ0200836.1"
                     /translation="MQTLAQAKQNFVYQVERIETEEKTKRHLQNIGIVAGSKIVVVNL
                     SGNNAILLVKSHRVAVSREILGQIFVNKKTASSESWTSLDQLKAGETGIVVAIHGQGA
                     VKRRLMDMGLTRNVKIVIRKLAPLGDPIELTVRGYELTLRKSEAELILIQREEKE"
     gene            7721..7945
                     /gene="nrdH"
                     /locus_tag="ACG8KR_001460"
     CDS             7721..7945
                     /gene="nrdH"
                     /locus_tag="ACG8KR_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HFQ0200837.1"
                     /translation="MNVKIFSKNNCMQCKMVKRFLTENQIAFEEVNIDEQPEAVDWLK
                     AQGFQSVPVITSDAETVIGFRPDQLRKLAS"
     gene            8104..10263
                     /gene="nrdE"
                     /locus_tag="ACG8KR_001461"
     CDS             8104..10263
                     /gene="nrdE"
                     /locus_tag="ACG8KR_001461"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000523276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFQ0200838.1"
                     /translation="MSLKNLQDVSYFKLNNEINRPVNGQIPLNKDKEALVAFFEENVK
                     PNTMTFPSIMEKINYLIDQEYIEAEFIQLYSPSFIEKLYQFLDEQHFSFKSFMAAYKF
                     YSQYALKTNDGSAYLETYEDRVAFNALYFANGDEELALALADEMIHQRYQPATPSFLN
                     AGRKRRGELVSCFLTQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSAKKENADEKIR
                     VKTLSLGVIVPDKFYELTRKNEDMYLFSPYSVEKEYGVPFSYVNITAEYDHLVANPNI
                     RKQKIKARDLENEISKLQQESGYPYVINIDTANRENPIDGKIIMSNLCSEILQVQTPS
                     VINGKQEYEVLGTDISCNLGSTNIVNLMQSPNFGQSVRTMTRALTFITDASDIDVVPP
                     IQNGNKLNHTIGLGAMGLHTYLAKEQMEYGSEDSLDFTNIYFMLLNYWTLVESNNIAR
                     ERKQVFHNFEKSAYADGTYFEKYVTGEFQPKSEKVKALFEGIFIPTAEDWNALKQAVM
                     KDGLYHQNRLAVAPNGSISYINDTSASIHPITRMIEERQEKKIGKIYYPAPYLSNETI
                     PYYTSAYDMDMRKVIDVYATAQQHVDQGMSLTLFMRSEIPEGIYEWKTTTKQTTRDLN
                     ILRHYAFHKGIKSIYYVRTFTDDAEEIGSNQCESCVI"
     gene            10313..11278
                     /gene="nrdF"
                     /locus_tag="ACG8KR_001462"
     CDS             10313..11278
                     /gene="nrdF"
                     /locus_tag="ACG8KR_001462"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005971 - ribonucleoside-diphosphate
                     reductase complex [Evidence IEA];
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFQ0200839.1"
                     /translation="MSETYYEAINWNEIEDIIDKSTWEKLTEQFWLDTRIPLSNDLDD
                     WRTLSQLEKDTVGHVFGGLTLLDTVQSESGMDQLRKDVRTPHEEAVLNNIQFMESVHA
                     KSYSSIFSTLNTKKEIEEIFDWTNTNPYLQKKAERINEIYKHGTPLEKKIASVFLETF
                     LFYSGFYTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEAEQETL
                     KDWMYNLLYELYENEERYTEELYDPIGWTEEVKTFLRYNANKALMNLGMDPLFPDTAN
                     DVNPIVMNGISTGTSNHDFFSQVGNGYLLGHVEAMKDDDYLIGLE"
     gene            complement(11367..12506)
                     /locus_tag="ACG8KR_001463"
     CDS             complement(11367..12506)
                     /locus_tag="ACG8KR_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFQ0200840.1"
                     /translation="MQSRFKQSKLFFWTVELVLTIIGVYFILQMPNIFSPILKMLSAI
                     FLPLLVAGFIYYMFDPIVVFLEKHRIPRVFGFLLTFLVVIILVVLTVMNVVPQLMEQT
                     IQLTQSLPMYADETSKWLNELAQREEFKNFNLEEQLASANLSLSNLFNIILVSLSASV
                     SKIISFMMQFFILLFTVPFILLFMFKDGHKFIDALSHFFPKAIRKELRQTVKELNETL
                     SAYISSTILDALIIGVMSFLAMRIFDQPYSLLLAVICGITNIIPYVGPFIGAVPAIIV
                     GLFISPFQALYMALSILVIQQLDGNLIKPLLFGKSMNIHPLTIILVLIGAGSVAGILG
                     MLICIPVYAVIKTIILNIRKIYLLRRMEHVSEKLDSPKVLEETSE"
     gene            complement(12815..13528)
                     /locus_tag="ACG8KR_001464"
     CDS             complement(12815..13528)
                     /locus_tag="ACG8KR_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HFQ0200841.1"
                     /translation="MNDMVLTIPEIILRLGIAMLFGGAIGFERQYKNRPAGMRTHILV
                     CMGACIIALIQVEIASGALRDALEYPQLAGTIRSDEARLIAQVVSGVGFLGAGTIIVT
                     KQSVTGLTTAASLWAIAGLGIAIGMGYYSIAIISFIGIAIALTVVKRVIHVPTTKKLE
                     IQFIHRKETKDFLTDYFEEKNIVIEDVNFDVKFVDDYRVYKNIYTIDLPKGLTYADVI
                     EELSVHKNITKLHLVSIAQ"
     gene            complement(13782..14483)
                     /gene="nagB"
                     /locus_tag="ACG8KR_001465"
     CDS             complement(13782..14483)
                     /gene="nagB"
                     /locus_tag="ACG8KR_001465"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HFQ0200842.1"
                     /translation="MEIIRVKNAEEGGKKAFEIIKEGMNKGAKVLGLATGSTPVTLYK
                     EMTSSDLDFSEMTSVNLDEYVGLGGSDEQSYRHFMDVQLFDKKPFKETFVPDGKAEDL
                     EAACKKYDQIIDAHPIDIQILGIGQNGHIGFNEPGASFDGTTSVVDLTESTINANKRF
                     FDKAEDVPTKAVSMGIGSIMKSKEIVLMAFGESKADAIKGMIEGPVTNHLPASVLQNH
                     DNVVVIIDEEAASKL"
     gene            14717..16249
                     /locus_tag="ACG8KR_001466"
     CDS             14717..16249
                     /locus_tag="ACG8KR_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFQ0200843.1"
                     /translation="MSILTLNNITQQFGDKILYEGVDLQLNAGEHLGLIGQNGAGKST
                     LIKIITGEILPDDGQIHWQKNIHKGYLDQYVEVDETLTIEEFLKTAYAKEYEKEEKIA
                     FLYQTYSETFDDSLLEKAGKLQTELDQGVFYQIDTLVAEMSSGLGIDVLGLDSLLKNL
                     SGGQRSKVILAKLLLENPDVLILDEPTNHLDDQHIQWLTQFLQDFDGAYIVISHDQEF
                     LDQITTHIADIEFGKITKYTGHLKQALKQKEQNRESYLRQYHAQQKQIEKTEAYIRKY
                     KAGSRSTMAKSRQKQLDKIERLTPPASASKAVFDFPFSPIVTTLAVETTDLVIGYDRP
                     LLAPINLTIRFGENIAIRGFNGIGKSTLLKTLIGEIEKISGDFHFPDNTKINYFSQDL
                     YWENPLETPLQYLSSQFPKATIKELRRQLAKAGLVNQLASEPLTTLSGGEQTKVKLAQ
                     LTMNQGNLLILDEPTNHIDQETKESLQDGIQKFPGTVIIVSHEQEFYQDLVQRVIEIE
                     PK"
     gene            complement(16440..16619)
                     /locus_tag="ACG8KR_001467"
                     /pseudo
     CDS             complement(16440..16619)
                     /locus_tag="ACG8KR_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(16639..>16795)
                     /locus_tag="ACG8KR_001468"
     CDS             complement(16639..>16795)
                     /locus_tag="ACG8KR_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HFQ0200844.1"
                     /translation="SSLEKLGCQNFNSRSVKGLESRFESILLAYSVLLSRAQRRFEGT
                     LRYSLGY"
BASE COUNT         5214 a         3459 c         2903 g         5219 t
ORIGIN      
        1 ttaaaggact gggatctaat tcatattttt gtttttctaa ttttaaatat accttttgta
       61 aaacctcaag aatatcagaa tatttaggat ctttacttat agatatatca ttatataatt
      121 catgaacaat ttgcttagca ttttttttca ttttagcact tccctggaat tgctccacct
      181 aaaaaaccac ctatagacat tcctgcaata caagtagttg ccttggccca atcgaccgta
      241 catttatttt tagtgcaata cactccattt ccataatatt ttccactatg agtggtaccc
      301 ccatagataa gttgtgtctc tttaatagac aaaattttta aatgtttcat aatctaatca
      361 cccctaaatt aatgttttta gactattatt ctaacattca ttaaatttca aaaaacaatt
      421 ccttcttgaa acgtagcaaa ataattcctg aataagaatt taatcacttt taagtatata
      481 aactatgtat attctttaat attttttgag aatgcacttc gtacataata aactttaatc
      541 acattagtct ttcataccta cgatcgatct ctttgactcg cacccataaa ttcataatag
      601 tttgtcggcg tctttgaacg tttcgaagat gagctacgca ttctcttttt ttatagacaa
      661 aaaagataga gacgaacatg gagcttccaa atttggatat ttgatccaat tttggaagct
      721 ccatttattt cgtgtctgtc tttttatttt ttccagcttt cgactaattc ttggttctct
      781 ttgatccagt cttttgctgc ttgctgtgga tcttttccat tgttgatttc caacataact
      841 ttttccatat ctttttctga ccaatggaaa ttgtctaata ctttgtaagc ttctggctgg
      901 tcttctttca atccttttcg aaccatcgta tggatctgct cttcgcttcc cattccgttt
      961 tcaggatctt ctaagtattt cagatcatat ttggcaaaca tccaatgagg tgtccatcct
     1021 gtaactacaa ttggttcgtg ttttttgatc gcttgaccta gtgcaacggt cattgctcct
     1081 gaagaagatg tctctacttt ccagtctttc aagttgtcat atttttggat cgtgttttca
     1141 gcagccgtaa ctacaccagc tcctggctcg attcctatga ttttcttacc tgcctgatcg
     1201 gttaactcat cgatcgaatt tacatccatg taggctggca ctgctaaacc aacttttgct
     1261 cctgtcaaat tggcacctag gtcttcgact tgatctttat attgtgcata ttgtgaacca
     1321 tgagttttag gtagccaagc tgaaaccatg gcatctgact cgccatttga aacagatttc
     1381 cacatgattg aattatctaa tggcgtcatc gttacatcat agcccatttg tttcaatact
     1441 tcaccgacta catttgttga agcaacttct gtatcccatt caacataaga taagttgata
     1501 cgttttgttt ctttggcaga tgaaaaactc cctataccaa tcgcacctaa gatcactact
     1561 actacagcac caatgatcag tccttgacgt tttttacttt tctgtgttgt tgctcctgca
     1621 gcttttttat tgtttggctg gttcaaatgc tgtgtaaaac ggtcaatgat gattgctaag
     1681 ataactaatg caaccccgtt gacaaatcca ttcccgactt gtgctctttg caatgctgat
     1741 aatacgccgc gtcctaagcc gggtgcaccg atcatagagg caataacgac cattgaaagt
     1801 gacagcattg ttgtttgatt gatccctgcc atgatcgtac ttttagctaa aggcaattct
     1861 agtttgaata gtttttgacg acttgtgctc ccgaatgaat cagaagcttc tactaattct
     1921 ttaggaactt gtcgaatccc taaattcgta aaacgaacgg ttggtggcag agcaaagatg
     1981 actgaggcaa atacccctgg aaccatcccg ataccgaaaa atgcaactgc tggaatcaaa
     2041 taaacgaatc ctggcattgt ctgcataaag tcaaggatcg gtttgataat actttgggct
     2101 ttactgcttt ttgccatcaa gatccctaat ggtaccccaa tgatgatcga gatcacacta
     2161 gataaaagaa caagtgtgac agtattcatc aaatcattcc atagaccttg gttataaata
     2221 aaccatagac cgattaatgt gaacaacgta agtcctggac gtttcttaga aataaagaaa
     2281 gctgctaccg tcaataacaa gataaacaaa ggagctggaa tcactaacag taaatttgta
     2341 atccccgaca tcactgcctg tccaatcgtt tgtaaaaatg aaaataggcc agaaaaagtt
     2401 gtcgtaaacc attctgtgat attttctacc cagtttgcca ctggtaattg atagttcaat
     2461 agattattca tgttcactca cctcgcttgt ttctgctaat gcttcaatca cactacctcg
     2521 gatgacgaca ccttttaatt ttccatcctc tacaactgca agtggagtcg gtgagtcata
     2581 aataatattg aaaatatcac ttaccaccat gtctttccca atcaccgtca cattttcatc
     2641 taggaagtcg atcaatggac gtttttgctt cctagcttct aacgcttgat ctgcagtgat
     2701 gatccctttc aggtgtctct ttctgtcaac agccatcagc atgctgactt cttcattacg
     2761 catccttgtc aatgcaacgt ttggtccatc agattcgata ttcgtcgtca atgcaggtac
     2821 catgatgttt tgagccgtaa gcactttgga acgatcgact tcttccacaa attcacgtac
     2881 ataatcattt gctggattgg tcaatatttc ttcacctgtt ccgatctgca tgatttcgcc
     2941 gtctttcatc aaggcgattc tgtccccaat ccgtaaagct tcattcaaat catgggtaat
     3001 gaaaataatc gttttttgaa cattggcttg caggtccagt aattcgtctt gcatttctcg
     3061 ccggatcagc ggatcaagag ccgagaacgc ttcgtccatc agcaagatct caggatcatt
     3121 tgctaaagct ctggctaggc caacccgttg ttgcatccca ccagataact gactaggata
     3181 ttggtcttta aatgaaagca aactagaatt ttctaatgct ttttctgctt tttcttgtcg
     3241 ttcttcttta ggtacaccac ggacttctaa cccgtattct gtattttcca agatcgttcg
     3301 atgtggaaac agaccgaagt tttggaaaac catattgatt ttatgacgac ggacttctcg
     3361 caattcttct ttgcttaact tcgatacatc ttctccatca atatagatac tgcccgaagt
     3421 tggctcaatc agacgattca atagacggat caatgtggat ttcccactcc cggaaagccc
     3481 catgatcacg aagatttctc cttcttttac gtcaaaattc acgtcataca caccaaccgt
     3541 tgcaccggtt ttctttaaaa tctccgtttt cggctggtga tcattcatca tatctaaagc
     3601 ggcttgtgtc tttttaccaa atatctttgt taaatgtttt acttctactt ttaccaaaca
     3661 aattctcctt ttttatgtaa attcattttt ctttctgaaa aaagtgacca cactatgtta
     3721 acatcatgta gtcactttgt caaaaatgaa agaaatttgc ttgtccaaac tgaaaaaaac
     3781 ttggctaaat ctttgtaaat aaaaggttta gccaagttaa tattttttta atatttcaca
     3841 caatcattct gcaagctgat gctccgggta aaaaaagttt gtcatacgct gtagtgtata
     3901 ttcatttcca ataaaatgaa cgatgtcccc tgccgtaaat ttagcatagg ggccaggtga
     3961 aacaagcagt tcttcattat gagaaattgc gataactgtg gcgccagtcg cctgccagat
     4021 attcaactcg ctaattgaac ggtccagatg cattgctttt tctgataaag ataattcaaa
     4081 cggaagtaaa ggattcatct tatgtattcg cttcgtttga ttgaccagct gatccaataa
     4141 ttgagaaaaa tgattcagct catcttgttg cttctccaca cttgccatga tttctgcttt
     4201 tgtatcttgg atggattgta catcttgata ttgcaatagg aaatcttttg ctttttcctt
     4261 cgaagcaaca aatgccccac ttccatgccg tacttccatg attccaagat ctaccagcac
     4321 attgatagcc ttgcgggtag tctcaggaga agcattgtac gtactggcaa gtgttgagcg
     4381 ggcatggatc ttctcaccca ctttatatcg gttttcaacg atacgttcgg ctaaatcaac
     4441 agcaatctgc tggtagcgcg ggcggacgat ttttttcttt ccagacattt tctttcctct
     4501 ttcttttttt actttccctc atcttactat gctctagcaa aataagcaaa aaaggggaga
     4561 atagcctctt gcaacgactg atcatcgctt gatctgttcc ttttttactg ggcaagctgt
     4621 ttgacagtca tcacaattct taccggattt taaacgagta tagacaattg cgcctgcact
     4681 tccaaagatt aacacactta ataagaatgt cgccatccta ttcctctcct tctaatgaca
     4741 atatagtata aaactggttc ttggattttg gctgacggaa caaaacataa acgagtccta
     4801 tcatcagtaa aatagcaaga tacgtcccta acgctacctg tccaccctcc aataagtgtc
     4861 ccaactgata aatcacaaaa ctaactaaat aagctaaacc gcattgatag cctatggcga
     4921 tccaagtcca tttcttggca ttcatttccc gatggatagc cccaattgcc gcaaaacaag
     4981 gcgcacaaag taaattgaag gctaacaatg aataagctgc tgcgggagta tatgtgtgtt
     5041 gtagcacttg ccagatttct ctgccatttt cggatacttc cgcatgacca aacaaaatac
     5101 caaatgtgcc aataatattt tctttagcga ccaatcctgt aatcgctgcg actgttcctt
     5161 gccagtttcc ccaaccaagc ggctgaaaaa caactgccag gattcgcccg aaaaaggcta
     5221 agatactttg gtcttcagaa acaggttgag cataaaaatt ataagaagag ctgaaccaga
     5281 tcacgatact agaaacaaag ataatcgttc ctgctttttt aacaaaagac ttgctgcggt
     5341 cataggtttg tcttaggacc gctgaccatt gaggaaaatg atatgcgggc aattccatga
     5401 taaaaggagc tgtatctcca gcaaacaaac gtgttttttt caagctgatt ccagagaaaa
     5461 taattgcagt cataccaaga aaataggcag aaggagctac ccaagattga tgaggaaaaa
     5521 aggcccctgc tactaaggca ataatcggca actttgcaga acaaggcatg aaggttgtga
     5581 ccatgatcgt caaacgacga tcttgctcgt tttcaatcgt gcgactggcc atgacgcctg
     5641 gcactccaca tcctgtagca atcagcatag gaatgaagga ttttcctgaa agaccgaatt
     5701 tcctgaaaag tcgatccatg acaaaagcga tcctcgccat atatccgcag tcttccagaa
     5761 agctcaagca taaaaacaat ataatcaatt gcggtagaaa tcctaacact gctcccacgc
     5821 ctgcgatgat gccgttcaag atcagctcct gcatccatcc cgctacttgc cattcagcta
     5881 acaatctgcc aacatgttct ggtaaccatg taccaaacaa tgtgtcattg atccaatctg
     5941 tccccattgt tcctactgtt tgaatagcca gataatagat cagccacatc acaaatgcga
     6001 agatcggcaa ggctaaccaa cgattcgttg caatctgatc gattttgtca ctcatcgata
     6061 attggaaaga atcatttgac acaacacaga gtgtacatag ttgggtaata aattcataac
     6121 gttcattgac cagaatcgtc tcactatcgt cgtccaatag tttctctgtt aactggatca
     6181 gttgctcgat ctctttttgc tgagaaactg ctatctcgta atcctctctt accttctgat
     6241 ctcgttcaaa atatttcatt gcataccagc gtttcttgcc gctttgttct cctagtacat
     6301 cttcaatttc atgtaaagct gcttctaaac gtggatcata tgtacaggga acaggaatag
     6361 aaggccgctt ttttgcagac tcctgaatct tttcgattag tttttcgatt ccttgctttt
     6421 tcaatacgct gattggtaaa actggtacac caagaccata ggaaagcttt tctagattga
     6481 ttttttttcc ttcttttttg atgacatcca tcatattcag accaatgatc ataggaatgt
     6541 ctaaatccaa taactgcgta gttaaataaa gatttcgttc taaattggta gcatcaataa
     6601 tgttgatgat cagttcaggc tgttcctgaa ccaaataatc tcttgcaatc acttcttctg
     6661 gtgtataagg agacaaagaa tagatgccgg gtaggtcctg aatatgaatg ttcggatttt
     6721 ttttcaacga tccttctttt ttttctactg ttacgcccgg ccaattccct acatattgat
     6781 tcgaaccagt caagagattg aacaagctag ttttcccgct gtttggattt ccgactaacg
     6841 cgatattcat tctttttcct ccctttgtat caagattagt tcggcctctg actttcgtaa
     6901 cgtcagttca taccctcgca ctgtcagttc gattggatct cccagagggg ctagttttcg
     6961 gataactatt tttacatttc gcgtcaatcc catatccatt aaccgtcgct tcactgcgcc
     7021 ttgtccgtgg attgctacga cgatcccagt ctctcctgct tttaattgat caagagaagt
     7081 ccagctttca gaggaagcag tctttttatt gacaaagatt tgacctagta tttcccgact
     7141 tactgccact cgatgacttt ttaccagtaa aatggcattg ttgcctgata aattgacgac
     7201 gacgatttta cttccggcaa cgatcccgat attttgcaaa tggcgttttg ttttttcttc
     7261 tgtttcgatt cgttcgactt gataaacgaa attctgcttt gcctgcgcta aggtctgcat
     7321 attgattcct ctcttgtgaa ataaatcatg tattaaaatc attcattata tgttctaatt
     7381 ttcttacgtg ttacgcaaat aaatgagaat cattctcaca tagaatactc tcggtaattg
     7441 agaatgtcaa ttatacttgt gatattatga gcgaatcttt agtattgata gataaaaatg
     7501 agaaaaaaat atttcttcat agttttccac aattttttct ctattaaatt tgctattttc
     7561 ccacacgata cttgttttca gctctttttt caaaaacaca aaacttagta ttccacagtt
     7621 tccacaacac aatatgtagt gtttgttgtt tgactttttt actaagagtt ttatactatc
     7681 tattatagat aataagaatg tgttagagga ggcactcacc atgaacgtaa aaatcttttc
     7741 taaaaataat tgtatgcaat gtaagatggt caaacggttt ttaacagaaa atcaaattgc
     7801 ttttgaagaa gtcaatatcg atgaacagcc tgaagcagta gactggttga aagcacaagg
     7861 attccaaagt gttcctgtca ttacttctga tgcagaaaca gttatcggat tccgtcctga
     7921 tcagttgcgt aaacttgcca gctaacatga ctaaaagaat caaaacgtga tacatggatt
     7981 tgaagggctt gagacagcac cagtttttgt cgcaaacttc caaatccttt tttaaagctt
     8041 aaaaacaatg caatgatttg caatcaagca tcatcaaata cgaaatgaag gttgggcgta
     8101 cgtatgagtc ttaaaaattt acaagatgtc agttatttca agctgaataa tgaaatcaat
     8161 cggccagtca atggtcaaat ccctttgaat aaggataaag aagctttagt tgcctttttt
     8221 gaggaaaatg tcaaaccaaa tacgatgact tttccatcta ttatggaaaa aatcaattac
     8281 ttgatcgatc aagaatatat agaagcagaa ttcattcaat tgtattcacc atcatttatt
     8341 gaaaagctat atcaattttt ggacgagcaa catttttctt tcaaatcttt tatggctgct
     8401 tacaaatttt attctcaata tgcgttgaaa accaacgacg gttccgctta tctggaaact
     8461 tatgaagatc gagtagcctt caatgcgctt tactttgcga acggggatga agaattagca
     8521 cttgctcttg cagacgaaat gatccatcaa cgatatcagc cagctacacc ttctttcttg
     8581 aatgctggac gtaaacgtcg gggcgaattg gtgtcttgtt tcttgactca agtcactgac
     8641 gatatgaact ctatcgggcg ctcaatcaac tctgctttgc aattgtcccg tattggtggc
     8701 ggcgtaggga ttactttatc aaatcttcgt gaagccggcg ctcctatcaa aggatatgag
     8761 ggtgctgcaa gtggtgtcgt tccagtcatg aaattattcg aagatagttt cagctattct
     8821 aatcaattag gtcagcgtca aggagctggc gtggtttatt tgaacgtttt ccatccagac
     8881 attattgctt tcctttcagc aaaaaaagaa aatgcagatg aaaagattcg cgtaaaaact
     8941 ctttctcttg gcgtgatcgt acctgataaa ttttatgaat tgacacgtaa aaacgaagat
     9001 atgtatttat ttagtccata tagtgtcgaa aaagaatatg gtgtcccatt ctcttatgta
     9061 aatattacag ctgagtatga tcatctagtt gcaaatccaa acattcgcaa acaaaaaatc
     9121 aaagcgcgtg atcttgaaaa tgaaatctca aaattacaac aagaatctgg ctatccttac
     9181 gtaatcaata ttgatacagc gaaccgcgag aatccaatcg atggcaagat catcatgagt
     9241 aatttatgtt cagagatttt acaagttcaa acgccttctg tgattaacgg caaacaggaa
     9301 tatgaagtat taggaacaga tatctcatgt aacttaggct cgacaaatat cgtgaatttg
     9361 atgcaaagcc ctaactttgg tcaatctgtg cgtacgatga ctcgtgcatt gacatttatt
     9421 acagatgcat ctgatatcga tgtcgtacca ccgatccaaa acggaaataa gctgaatcat
     9481 acgattggat taggtgccat gggcttacac acttatcttg caaaagaaca aatggaatat
     9541 ggctcagagg attcattaga ctttacaaat atttacttca tgctgttgaa ttattggaca
     9601 cttgttgaaa gcaacaatat cgctcgtgaa agaaaacaag ttttccataa ttttgaaaaa
     9661 tcagcttatg ctgacggcac ttatttcgaa aaatacgtaa ctggagaatt ccagccaaaa
     9721 tcagaaaaag tcaaagcatt gtttgaaggc atttttatcc caactgctga agattggaat
     9781 gctttgaaac aagcagtaat gaaagatgga ctgtatcatc aaaatcgctt ggcagtagca
     9841 ccaaatggct caatttcata tatcaacgat acgagtgcaa gtattcatcc tatcactcgc
     9901 atgatcgaag aacgccaaga gaagaaaatc ggtaaaattt attacccagc gccttatcta
     9961 tccaatgaga cgattccgta ttacacttct gcttatgata tggacatgcg taaagtaatc
    10021 gatgtttatg caacggcaca acaacacgtc gatcaaggca tgagtttgac tttattcatg
    10081 cgttctgaaa tcccagaagg catttatgaa tggaagacaa caacgaagca aacgacacgt
    10141 gatttgaata ttttaagaca ttacgctttc cacaaaggaa tcaaatccat ttattacgta
    10201 cgtaccttta ctgatgatgc agaagaaatc ggaagcaatc aatgtgaaag ctgcgtgatc
    10261 taatatcata gatagattcg cttattcata ttttaattaa aggagcattc tcatgtcaga
    10321 aacatattat gaagcaataa actggaatga aattgaagat atcattgata aatccacttg
    10381 ggagaagctg acagagcaat tttggctaga tacacgtatc ccactttcaa atgacctaga
    10441 tgattggcgt acattgtctc aattggaaaa agatacagta ggacatgtat ttggcggttt
    10501 gacactgcta gatactgtcc aatctgaaag tggcatggat cagttgcgta aagatgtccg
    10561 cactccccat gaagaagccg ttttgaacaa tattcaattc atggaatccg ttcatgccaa
    10621 aagttattct tctattttta gtacactgaa tacgaaaaaa gaaatcgaag aaatttttga
    10681 ctggacaaat acgaatcctt atctgcaaaa aaaagctgag cgtatcaatg aaatctacaa
    10741 acacggtaca ccgctagaaa agaaaattgc cagcgtcttt ttagaaacat tcttgttcta
    10801 ttcaggtttt tatactcctc tttattattt aggaaacaac aaattggcta acgtagcaga
    10861 gatcatcaaa ctaatcatcc gagatgaatc tgtacatgga acctacatcg gttacaaatt
    10921 ccaattagga ttcaacgagt tacctgaagc agaacaagaa acgttaaaag actggatgta
    10981 caatcttctt tatgagcttt acgaaaacga agaacgttat acagaagaat tatacgatcc
    11041 aatcggctgg acagaagaag tcaagacatt cctacgctat aatgccaaca aagcattgat
    11101 gaacctaggg atggatccac tctttccaga tactgccaat gatgtcaacc caatcgtaat
    11161 gaacgggatt tcaacaggca caagcaacca tgacttcttc tcacaagtcg gaaacggcta
    11221 tttgttaggt catgtagaag cgatgaaaga cgacgattat ttgattggat tggaataagg
    11281 atttatttta attcaaaaat ttttcgaaaa ataaaaagag tttagcgtac agatatttta
    11341 aatgtacact gaactctttt ttatgcttat tcacttgttt cttccagcac ttttggactg
    11401 tctaactttt ccgaaacatg ttccattctt ctcaataaat agattttccg gatatttaat
    11461 attatcgtct taatcactgc atagacaggt atgcaaatca gcatgcctaa tattcctgcg
    11521 acacttcctg ctccaatcaa taccaagatg atcgtcaaag gatggatatt cattgatttc
    11581 ccaaataaca acggcttgat caaatttcca tctaattgtt ggatcactaa aatcgacaac
    11641 gccatataca aagcttgaaa tggagagata aacaaaccga caatgattgc tggtaccgct
    11701 ccaataaaag ggccgacata cgggataata ttcgtgattc cgcaaatgac cgcaagcaac
    11761 aagctataag gctgatcgaa tattctcatt gccaaaaagc tcattacacc aataatcaaa
    11821 gcatctaaga tcgtactgct gatataggca gataatgttt cgtttaattc ttttaccgtt
    11881 tggcgtaact ctttacgaat cgctttcgga aagaagtgag acaacgcatc aataaatttg
    11941 tgcccatcct tgaacataaa tagcaaaata aatggaacgg taaataacaa aataaaaaat
    12001 tgcatcataa atgaaatgat ttttgagaca cttgcagata aactgactaa tatgatattg
    12061 aacaaattac taagagataa atttgctgat gctaattgtt cttccaaatt gaaattttta
    12121 aactcttccc gctgcgccaa ttcatttaac catttgcttg tttcatctgc atacattggc
    12181 aagctctggg tcaattgaat tgtctgttcc attaactgag gcactacatt catgacagtt
    12241 aaaacaacta aaatgattac gaccaaaaac gtcaataaaa aaccgaacac tctcgggatc
    12301 cgatgctttt cgagaaaaac aacgattgga tcgaacatat aatagataaa accagcaaca
    12361 agcaatggta aaaaaattgc cgaaagcatt ttcaatattg gactaaaaat attcggcatt
    12421 tgtaaaataa agtacacgcc gatgatcgtg agtactagct ccactgtcca gaaaaataat
    12481 tttgattgtt taaaacgtga ttgcatacat ctcctccaag gtcctttgat acttctattc
    12541 tatcatttaa caaagagtta cgcgctgaga ttttcatttg cttaaaaaaa acaaaaaaaa
    12601 taaacatatt ttaatgagta gacgagtact taaacaaaaa cacggtttgg agtagtgaca
    12661 aaaattatac tgcagtccct ttttccaaat aaacggtgtt caaaagcttg catcttcgta
    12721 ataagcccag ccaaacgaaa aatataagga actattttta tttggctggg cttattactt
    12781 gatgtaggac atctatttta caacctcaga tcatttattg cgcaatactg actaaatgca
    12841 actttgtgat atttttgtgt acggacaact cctcgatcac atcggcataa gtgagccctt
    12901 ttggaagatc aatcgtatag atatttttgt acactcggta gtcatcaacg aattttacgt
    12961 caaaattcac atcttcgatt acgatatttt tctcttcaaa ataatcggta agaaaatctt
    13021 tggtttcttt tctatggata aattggattt ctaacttttt agtcgtaggt acatggatca
    13081 cgcgtttcac tactgtgagt gcaatagcaa tcccgataaa actgatgatc gcaatggaat
    13141 agtaacccat gccgatggcg atccctaatc cagcaattgc ccataaagaa gcggcagtcg
    13201 taagtcccgt caccgattgt ttcgtgacga tgatcgttcc cgcgcctaag aaaccgacac
    13261 cactcaccac ttgagcaatc agccttgctt catctgaacg aatcgttcca gcaagctgtg
    13321 gatactctaa agcatctctt aatgcaccac tagcgatctc aacttggatc aatgcaatga
    13381 tacatgctcc catacaaact aaaatatgcg tacgcatacc tgctggacga tttttatatt
    13441 gtcgttcaaa accgattgct ccgccaaata gcatagcgat ccctaaacgt aaaataatct
    13501 ctggaattgt caataccata tcattcatct aaacaccctc cagtccaata tatcatgtct
    13561 gaaaggagca gacaaaaaaa gtgcaatttt tacctcttac gaaaaaagga ctgtgacaaa
    13621 atttttgtca tagtcctttt tatgaataaa cggtgttcaa tcagctgacc ctcggtatta
    13681 ctcaacaaac ggcaaaatat ggggagctat ttttcctttg tcttctctaa tactcaggtc
    13741 aaaacgctga tttcacaacc tctttagcct actattgaaa attacaactt acttgctgct
    13801 tcttcatcga tgatcacaac aacattgtcg tgattttgca atacacttgc tggcaaatga
    13861 ttcgttacag gtccttcaat catccctttg attgcatcag ctttgctttc gccaaatgcc
    13921 atcaagacga tttctttact tttcataatt gaaccgattc ccattgaaac agcttttgtt
    13981 ggtacatctt ctgctttatc gaagaatcgt ttgtttgcat tgattgttga ttctgttaag
    14041 tcaactactg aagtcgttcc gtcaaatgaa gcaccaggtt cattgaaacc gatatgtcca
    14101 ttttgcccga ttcctaagat ttggatatcg attggatgtg catcgatgat ttgatcatac
    14161 tttttgcatg ctgcttccag atcttctgct tttccatctg gtacaaacgt ttctttgaat
    14221 ggttttttat caaataattg gacatccatg aaatgacggt agctttgttc atctgaaccg
    14281 cctagtccaa cgtactcatc taagtttaca gaagtcattt cactgaaatc caaatcgctt
    14341 gaagtcattt ctttatataa agtaactggt gtgcttcctg ttgccaatcc taatactttt
    14401 gcacctttgt tcataccttc tttgatgatt tcaaatgctt tttttccgcc ttcttcagcg
    14461 ttttttacac gaatgatttc catagtgatt gctcctttat attttggtat agcccaatta
    14521 tagcattctt tttttctttt tacaataatt agtatagacc aaattgcatt tttttcattt
    14581 tcttgacaat aaataggaat ccgtgttagg tttcataaga tggttcaatc aaacaatcca
    14641 tgttgcatga aaaccaagca agtgattcac tgtggacgct ttatgtctgc agttttttta
    14701 tgaaaaagga gttattatga gtatactgac attaaacaac attacccagc aatttggcga
    14761 caaaatcctc tatgaaggtg tcgatctgca attaaatgct ggcgaacatc ttgggctgat
    14821 tggacaaaac ggagccggta aaagtacatt gattaaaatc atcactggtg agatcttacc
    14881 agatgacgga cagatccatt ggcaaaaaaa tattcacaaa ggttacctcg atcaatatgt
    14941 ggaagtcgat gaaacattaa caatcgaaga atttctaaaa actgcgtacg caaaagaata
    15001 cgaaaaggaa gaaaaaattg cttttcttta ccaaacatat agcgagacct tcgatgattc
    15061 cttgctggaa aaagctggga aactccaaac ggaattagac caaggagtgt tttatcaaat
    15121 cgacacctta gttgctgaga tgagcagcgg gttggggatc gatgtcttag gtttagattc
    15181 actgttgaaa aatctaagtg gtggacaacg ttcaaaggtg attttagcaa aactactttt
    15241 agaaaaccca gatgttctga tcttagatga accaaccaat cacttagatg atcagcatat
    15301 ccaatggctg acgcagtttc ttcaggattt tgacggtgcg tatatcgtga tttcccatga
    15361 ccaagaattt cttgatcaga tcaccaccca tatcgcagac atcgagtttg gaaagattac
    15421 taagtatact ggccatctaa aacaagcatt gaaacaaaag gaacaaaatc gcgaaagcta
    15481 tttgcgccaa tatcatgctc aacaaaaaca aatcgaaaag accgaagctt atattcgaaa
    15541 atacaaagcc ggttcccgat caacgatggc aaaaagccga caaaaacaat tagataaaat
    15601 cgaacggctg acgcctccag cttcagcaag taaagctgtc tttgattttc cattctcgcc
    15661 tattgtcacg acattagcag tggaaacaac tgatttagtc atcggatatg atcgaccgct
    15721 acttgcaccc atcaatttga ccatccgctt tggtgaaaat attgccattc gaggcttcaa
    15781 cgggattgga aaatctactt tattaaaaac actgatcggt gagattgaaa aaataagtgg
    15841 cgatttccac ttcccagata acacaaaaat caattacttt tcacaagatc tgtactggga
    15901 aaatccatta gaaacaccac tacaatattt aagcagtcaa tttccaaaag cgactatcaa
    15961 agaattacgt cgccaactag ctaaagcagg gttagttaat caattagcaa gtgaacctct
    16021 cactacatta agtggtgggg aacaaactaa ggtgaaactt gcacagttga caatgaacca
    16081 aggtaattta ctgattctcg atgaaccgac gaaccatatc gatcaagaaa cgaaagaaag
    16141 cttacaagac gggatacaga aatttccagg aacagtaatc atcgtctctc atgaacaaga
    16201 attttatcaa gatcttgtgc aacgagtcat tgagattgaa ccaaaataaa cgtttgattg
    16261 aatagttgaa aaaaaggagc tgtgacaaaa gttttgtcac aactccttct tctgaataaa
    16321 tggtggccaa ttagctgacc ttcggtatta gctcaaaaaa cggcaaaata tgaggagcta
    16381 tttttatttg gctattctta ttacttgatg cagaacagct tttcacaacc tcttttcgtc
    16441 tattttttat tttcgagatt tctctttcct ttaaaaacaa ctgtttgaaa gaagaacgca
    16501 catccttgaa aaacaagccc gctaaccgtt acacctaacc agccatactg ctgccacatt
    16561 gtcgtcccta gcaatgaacc agccgcacct ccaataaagt acataaacat aaatacagac
    16621 ccgtggtgct agttaatttt aatatcctaa agaatatctc aaagttcctt caaaacgtcg
    16681 ttgtgctcga cttaatagaa cactgtaagc cagtaatatg ctttcaaatc tactctctag
    16741 ccctttaaca gaacgtgagt tgaaattttg acaacctaat ttttctaagg aagaa
//
LOCUS       DAZKLD010000055        16723 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.055, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000055 DAZKLD010000000
VERSION     DAZKLD010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16723)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16723)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16723
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.055"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            468..1640
                     /locus_tag="ACG8KR_001469"
     CDS             468..1640
                     /locus_tag="ACG8KR_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS256 family transposase"
                     /protein_id="HFQ0200845.1"
                     /translation="MTQVHFTLKSEEIQSIIEYSVKDDVSKNILTTVFNQLMENQRTE
                     YIQAKEYERTENRQSQRNGYYERSFTTRVGTLELKVPRTRDGHFSPTVFERYQRNEKA
                     LMASMLEMYVSGVSTRKVSKIVEELCGKSVSKSFVSSLTEQLEPMVNEWQNRLLSEKN
                     YPYLMTDVLYIKVREENRVLSKSCHIAIGITKDGDREIIGFMIQSGESEETWTTFFEY
                     LKERGLQGTELVISDAHKGLVSAIRKSFTNVSWQRCQVHFLRNIFTTIPKKNSKSFRE
                     AVKGIFKFTDINLAREAKNRLIHDYIDQPKYSKACASLDDGFEDAFQYTVQGNSHNRL
                     KSTNLIERLNQEVRRREKIIRIFPNQTSANRLIGAVLMDLHDEWIYSSRKYINFDK"
     gene            1869..2822
                     /gene="yidC"
                     /locus_tag="ACG8KR_001470"
     CDS             1869..2822
                     /gene="yidC"
                     /locus_tag="ACG8KR_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HFQ0200846.1"
                     /translation="MNKWKNWLLGSGLVSILLFLSGCVRMDSNGNPDTSGIVYRVLVH
                     PMGQAITYLVENFNWSYGWAVIAMTVIVRIIILPLGISQSKKTMIQSEKMQALKPQVE
                     AAQQKLKAATTREEQMAAQAEMQQVYRENGLSMTGGIGCLPLLIQMPIFSALYFTARY
                     TEGIRESSFYGIDLGSPSMVLVAIAGGAYLLQGYISTIGIPEEQKKTMRTMLIVSPAM
                     IVFMSISAPAGVTLYWVVGGVFSCLQTFITNVIMKPRLKAQVAEELKQNPPKQVVTPR
                     KDVTESAAKPADNKQATKQIKQPKNSTNNKGRNAGRQQKRK"
     gene            2944..4153
                     /locus_tag="ACG8KR_001471"
                     /pseudo
     CDS             2944..4153
                     /locus_tag="ACG8KR_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012008915.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182 family transposase"
     gene            complement(4198..4598)
                     /locus_tag="ACG8KR_001472"
                     /pseudo
     CDS             complement(4198..4598)
                     /locus_tag="ACG8KR_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723846.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(4845..5279)
                     /gene="tnpA"
                     /locus_tag="ACG8KR_001473"
                     /pseudo
     CDS             complement(4845..5279)
                     /gene="tnpA"
                     /locus_tag="ACG8KR_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005576373.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(5457..5823)
                     /locus_tag="ACG8KR_001474"
                     /pseudo
     CDS             complement(5457..5823)
                     /locus_tag="ACG8KR_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387104.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
     gene            complement(5837..6122)
                     /locus_tag="ACG8KR_001475"
                     /pseudo
     CDS             complement(5837..6122)
                     /locus_tag="ACG8KR_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311319.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
     gene            complement(6131..6322)
                     /locus_tag="ACG8KR_001476"
                     /pseudo
     CDS             complement(6131..6322)
                     /locus_tag="ACG8KR_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018659219.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(6322..6798)
                     /locus_tag="ACG8KR_001477"
                     /pseudo
     CDS             complement(6322..6798)
                     /locus_tag="ACG8KR_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290865.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
     gene            complement(7266..7619)
                     /locus_tag="ACG8KR_001478"
     CDS             complement(7266..7619)
                     /locus_tag="ACG8KR_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0200847.1"
                     /translation="MAMSEKAKKYLKEIKGAKTIPELKDVEIAIKRDGILAWAEFTKL
                     NEAVEEKKVALRKKKQETSLQEILFWAYKKESDKLLKMMDEGADKDAIQMQVQRYDSI
                     GQIIAEADLEDEYEV"
     gene            complement(7747..8157)
                     /locus_tag="ACG8KR_001479"
     CDS             complement(7747..8157)
                     /locus_tag="ACG8KR_001479"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0200848.1"
                     /translation="MEEIMQMILGNAKFQLGQVVSTPAVLETFNPLQTANGIPEALSN
                     LWFRQEISIAEIEDAVLDAKNGKELANSLNRIKLFRKFTLDRELDGMVRLKGTDVWKN
                     ISYLKVYTATPPKKPTPPRFQSNGWSLFFSHKSA"
     gene            8316..8507
                     /locus_tag="ACG8KR_001480"
     CDS             8316..8507
                     /locus_tag="ACG8KR_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0200849.1"
                     /translation="MIIRDDHIYTCDSCHYSFPADEQPERCPDCEKTATRLDTEIETE
                     DYYRVRAEIKAEIKALNAG"
     gene            complement(8549..9415)
                     /locus_tag="ACG8KR_001481"
     CDS             complement(8549..9415)
                     /locus_tag="ACG8KR_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFQ0200850.1"
                     /translation="MEELFSNALKSVKSNRVLYTASFFARSSLLYLQEIGQLTALKAH
                     TSRRTGLSSYLCFLVEVGSGTLEYKGQKYTLQVGDMVFIDCLKPYVHESSEDDLWTLR
                     WCHFNGIGMTEVYKKYLERGGQPVFKTSKTDKYADLLRSLHMVAEGQEYVRDMKINVL
                     LSELLMHLMEDSWNPDKVPNFSTKKMELQNIKQYLDDHYTEKITLDQLSAEFFINKQY
                     LAKIFRETYGTTVVGYLNQKRITQAKHLLRFTNLSMEEIATKIGITDANYFSRTFFKV
                     EGVRPSEFRKMW"
     gene            complement(9439..11091)
                     /locus_tag="ACG8KR_001482"
     CDS             complement(9439..11091)
                     /locus_tag="ACG8KR_001482"
                     /EC_number="5.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HFQ0200851.1"
                     /translation="MNYKVEYERWLNHANADIIEELRSYSEKDIEDSFYKNLSFGTGG
                     LRGTIGAGTNRMNVHTVGKASQGLADYLNKNYEAASVVVGYDSRIKSDVFAKTAADIF
                     AANGIQVHLWPELLPVPTVSFATRYLKADAGVMITASHNPSKYNGYKVYGADGCQITT
                     EAATAILAEIEKLDIFADVKMDGDASKIASIQSEVLTAFIEEVKGQSVLFGEEVDKNV
                     AIVYSPLNGTGLVPVTRTLAEMGYSNVTVVEEQRLPDGNFPTCPYPNPEIKEAMSLGM
                     EYAKKYNADLLLATDPDCDRVGIAVKSAHGDYVLLSGNETGLLLLDYICSQRIKHGKM
                     PADPVMVKTIVTMDLGERIASNYGVRTIDVLTGFKFIGEQIGMLEKAGKVDSYIFGFE
                     ESYGYLSGSYVRDKDGVNAAYLICEMFSYYKTQGISLLDKLNEIYTKYGYCLNTLHSF
                     EFDGAAGFTKMQEIMVEFHKGLDIIGGKKVIETLDYSKGLNGLPKSDVLKFMLEDNCS
                     VVVRPSGTEPKLKTYISVSAENKEAAEAMEAIMAAELAEMFE"
     gene            complement(11096..11998)
                     /locus_tag="ACG8KR_001483"
     CDS             complement(11096..11998)
                     /locus_tag="ACG8KR_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I phosphomannose isomerase catalytic
                     subunit"
                     /protein_id="HFQ0200852.1"
                     /translation="MMNLQKELRCLLLRKLGMLYAPGWTELCCVWSEYQGMTLTEVLQ
                     EHPEFLGTHPQTKGELPILIKFIDAKKDLSVQVHPTDEYAIEKENGQLGKTEMWYVLD
                     ASLIYGLQKITEEAVIREAIAQGTVERYLQKVPVKKNDVFFIEAGTIHAIGAGALIAE
                     IQENSNLTYRLYDYDRVDKNGKKRELHVDKALEVANLKGLSEPKQPLRVLKYRAGVAA
                     ELLCRCKHFEVSRMIVNTERRQTVHYRADSISFRVLLCVNGCGTVRFDNESLDIYKGD
                     CLFVPADSTEISIHGSIQFLDVRG"
     gene            complement(12082..13395)
                     /locus_tag="ACG8KR_001484"
     CDS             complement(12082..13395)
                     /locus_tag="ACG8KR_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HFQ0200853.1"
                     /translation="MNIVLLSGGSGKRLWPLSNDIRSKQFIKIFKTEDGYESMVQRVY
                     RQIKKVDNDATVTIATSKTQVSAIHNQLGEDVGVSVEPCRRDTFPAIALATAYLVDVM
                     GIDPEESVVVCPVDPYVEDDYFEALKSLSDQADKGEANLVLMGIEPTYPSEKYGYIIP
                     ESAEQTAFVNTFKEKPSEAVAKEYIAQGALWNGGVFVYKLKYVMERAHQLIDFVDYQD
                     LFNKYDTLTKISFDYAVVEHEDKIQVQRFAGMWKDLGTWNTLTEGMEESIIGKGVMNE
                     NCDGVHILNELDVPVLAMGLHDVVICASREGILVSDKEQSSYIKPFVDKFEQQIMFAE
                     KSWGSYKVMDVEAESMTIKVTLNASHSMNYHSHKNRDEVWVVLAGTGKTIVDGYEQVV
                     KAGDVITMSAGCRHTIMADTELKLIEVQLGKDINVKDKQKFELEY"
     gene            complement(13463..14407)
                     /locus_tag="ACG8KR_001485"
     CDS             complement(13463..14407)
                     /locus_tag="ACG8KR_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014424865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase family protein"
                     /protein_id="HFQ0200854.1"
                     /translation="MMEKDAKIYVAGHRGMAGSAIVRELNRQDYNNIITRTHKELDLC
                     RQDAVEAFFAQEKPDYVFLAAAKVGGIIANQNALADFMYENMILEMNVINSAWENGCK
                     KLQFLGSSCIYPRMAPQPMPESCLLTSELEKTNEAYALAKISGLKYCEFLNKQYGTDY
                     ISVMPTNLYGPNDNYHPTHSHVLPALIRRFHEAKEAGLPTVTCWGDGSPLREFLYVDD
                     LANLCVFLMNNYSGDETVNAGTGKELSIKELTEMVAKVIGYEGEILWDTSKPNGTPRK
                     LLDVSKATKLGWTYKTELEDGIRLSYEDFLNNPMRAER"
     gene            complement(14444..15529)
                     /locus_tag="ACG8KR_001486"
     CDS             complement(14444..15529)
                     /locus_tag="ACG8KR_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFQ0200855.1"
                     /translation="MNKHICFYLSSHGFGHIARNLPIIMATLCRCEAKITLVCGANHL
                     DFAKRNLEQELPKELIDRVRYRPINTDIGLINREGTLLVDTGKLTEICEYFLAKVPSL
                     AKTEAEWLKREAVSAVLCDMPIWAMDACDMAGVPFLYVGNFTWTELYREFLPEHIWKE
                     YAKRYGKIQHTMLYALNNEEMLEFLQNSEKTPTSVVARPIHNDEVQRIKSQHEKPPVF
                     VALGMSARFKQPIDVSHAPYDFITTLGVPLVGENVTVISNSTPNTQDYIAAADYVITK
                     AGWGTVAECILAQRPMALFARDTVLEDRTTICKLQKLGLAISVTPENLHRVERVIEDL
                     KQLDTSNFDTFYDAASEIADKLISLMQ"
     gene            complement(15537..16583)
                     /gene="gmd"
                     /locus_tag="ACG8KR_001487"
     CDS             complement(15537..16583)
                     /gene="gmd"
                     /locus_tag="ACG8KR_001487"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0019673 - GDP-mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HFQ0200856.1"
                     /translation="MKKALITGITGQDGSYLADFLLEKGYEVHGITRRASISNTARID
                     HLMGKITLHDGDLSDSSSLIRIISIVQPDEIYNLAAQSHVQVSFDVPEYSGDVDALGV
                     MRILEACRILGLTKKTKIYQASTSELYGKVEEVPQRETTPFHPYSPYAVAKQYGFWIT
                     KEYREAYGMFAVNGILFNHESERRGENFVTRKITLAAGRIAEGLQDHLELGNMDSLRD
                     WGYAKDYVECMWMILQHETPEDFVIATGEQHTVRDFTEKAFAANGMTIRWEGTGIDEK
                     GYDVETGKMLVCVNPQWFRPTDVDNLWGDPTKAKTVLGWNPQKTSYEELVNIMAKHDR
                     ELAKREAAMKNAVK"
BASE COUNT         4727 a         3898 c         3268 g         4830 t
ORIGIN      
        1 aaaaagttca aaagatcaag tgtgcgcttc gctcccactc ccctcgacgt tttagtagcc
       61 tttccctcac ttcgttcagt ccaagccaac taaaaagttt tcgggataca ttctccttct
      121 cttcctaata atcaattaaa attttaatct ttatatttct gctaatcact caggaaacag
      181 tacagaaaaa caaacacgta tcatagatat aaatgtaatg gcatagtgcg ggttttattt
      241 tcagcctgta tcatagctaa acaaatcgag ttgtgtgtcc gtttttgggc gttctgctag
      301 cttgtttaaa gtctcttgaa tgaatgtatg ttctaagtca aaaaactctg acagcgcctt
      361 tatatagata aagtccgtat aattgtgtaa aagtaaaaag gccatataac agtcctttta
      421 cggtacaatg tttttaacga caaaaacata cccaggagga cttttacatg acccaagtac
      481 attttacact gaaaagcgaa gagattcaaa gcattattga atattctgta aaggatgacg
      541 tttctaaaaa tattttaaca acggtattta atcaactaat ggaaaatcaa cgaacagaat
      601 atattcaagc aaaagaatat gaacgaacag aaaaccgaca aagtcaacga aatggctatt
      661 atgagcgcag ctttacgaca cgtgtaggca cgctagaatt aaaagtaccc agaacacgtg
      721 atggccattt ttcacccaca gtgtttgaac gttatcaacg aaacgaaaaa gccctcatgg
      781 cttcaatgtt ggaaatgtat gtatcaggcg tttcaactcg taaagtatca aaaattgtgg
      841 aagaactttg tggtaaatcc gtctctaagt ccttcgtttc tagcttaaca gaacagctag
      901 aacctatggt taacgagtgg cagaatcgtt tattatcaga aaaaaattat ccttacttaa
      961 tgaccgatgt actctatata aaagtacgag aagaaaatcg agtactctca aaaagctgtc
     1021 atatagcgat tggaataacc aaagatggcg accgtgaaat tatcggcttc atgattcaaa
     1081 gtggcgaaag cgaagagacc tggacaacat tttttgaata cctaaaagaa cgcggtttac
     1141 aaggtacgga actcgttatt tctgatgcgc acaaaggatt agtctctgcc attagaaaat
     1201 ccttcaccaa cgtaagttgg caaagatgcc aagttcactt cctaagaaat atctttacca
     1261 ccattcctaa aaaaaattca aaatctttca gagaagctgt taaaggaatt tttaagttca
     1321 cagatattaa cttagcgcgt gaggctaaaa atcgattgat tcatgattat atcgatcaac
     1381 caaaatattc aaaagcttgc gcatcattgg atgatggatt cgaagacgcc tttcaatata
     1441 ccgtacaagg aaattcccac aatcgactaa agagtaccaa tctaattgaa cgactgaatc
     1501 aagaagtacg cagaagagaa aagattattc gcatcttccc caatcaaaca tcagccaatc
     1561 gcttaattgg agccgttctt atggacctac atgatgaatg gatttattct tcaagaaaat
     1621 acatcaattt tgataagtag aaatggtaaa aacattgtat agcattttac acaggagtct
     1681 ggacttgact cttggaatca ctcttttctt tctaagtatt tctttcttga ttttttgtct
     1741 tttttcaaaa tttttcgcta cgaaaaagaa cgaatattta tatgaaattt ctatgaagtt
     1801 gattgaattc catgcgcttt tttagtatag ttaagtttgt gtaaaaatta tagaagagga
     1861 agtattctat gaataaatgg aaaaactggt tattgggatc cggacttgtc agtatcctgc
     1921 tctttttatc tggctgtgtg cggatggatt caaacggaaa ccctgacact tcaggaatcg
     1981 tttatcgagt actcgtccat cctatgggcc aagcgattac atatctagtt gagaatttca
     2041 attggtctta cggctgggca gttattgcaa tgactgtgat cgttcgtatc atcatcttgc
     2101 cactaggtat cagtcaatcg aaaaaaacaa tgatccaatc tgaaaaaatg caagcgttaa
     2161 aaccacaagt tgaagcagca caacaaaagc taaaagctgc aacaacacgt gaagaacaaa
     2221 tggcagcaca agcagaaatg cagcaagtat atcgcgaaaa tggattgagt atgaccggcg
     2281 gaatcggctg tctgcctcta ttgatccaaa tgccgatctt ctctgccttg tattttacgg
     2341 ctcgctacac agaaggtatc cgtgaatcat ctttttacgg catcgatcta ggctcaccaa
     2401 gtatggtgct tgtggccatc gcagggggcg cttatctgct tcaaggatat atttcaacaa
     2461 tcggtattcc tgaagaacag aaaaagacga tgcgtacgat gctgatcgtc tctcctgcta
     2521 tgatcgtctt catgtcgatc agtgcgccag ccggtgttac actttactgg gtagtcggtg
     2581 gtgtgttcag ctgtctgcaa acattcatca caaacgttat aatgaaaccg cgtttgaaag
     2641 cacaagtggc agaagagttg aaacaaaacc cgccaaaaca agtcgttact ccaagaaaag
     2701 acgtgacaga atcagcagca aagccagctg acaacaaaca agcaacaaaa caaatcaaac
     2761 aaccaaaaaa cagtacaaac aacaaaggcc gcaacgcagg aaggcagcag aagcggaaat
     2821 aaaaatatat gacaaaaaac tgtagacaaa taaaaaaatt tctttgttct acagtttttt
     2881 tgttacaatt aaagaaaacg ttcgcatttc gaggaggaat gtataatgat gtccaaataa
     2941 tcaatgtata gtcgaatgga agaagacaaa atttcgctag atgcacttgt tcctaaaaat
     3001 catttagtcc gtaaaattga caaattattg tcatttgatt ttatttatct cattgttgaa
     3061 tcaacttact cgaccattgg tcgccctagc gttgatcctg tcgtcttagt aaaattagta
     3121 ttcattcaat atttatttgg aatttgttcg atgcgtcaga ccattaaata agttgaaaca
     3181 aacctagctt atcgttggtt tcttgggctt agtttaattg ataaagttcc tcatttttca
     3241 acttttggta aaaactatgc acggcgtttc agagaaaccg atttgtttga acagatattt
     3301 atcattattc tcgaataagc agttgatgct agatttattc atgaagaaat tttgtatatg
     3361 gattcaactc atattaaagc gaatgcgaac aaacgtattt ttacaagaga tatggcacat
     3421 aaagaagcca ttttatacca aaaacaatta gaagaagaaa tcaatgaggc aagaattgcc
     3481 gaaggcaaac gtccttttgg ttcaatcatt cgtcaggagg tggtcgaaag aaaatttagc
     3541 atcgcagatc cagaaagtgg ttattatgta aaaacagaac gagaaaaaca gtttgcttat
     3601 tctgctcata ccgttggtga tgaaaatggt tttgtattag atgtcatgat tacgcctgga
     3661 aatctacatg atagccgaat gctcgtgcca caaatcgaac gcgttaaatc acgttatggt
     3721 gtagctgcta atgctgcgta caaaacacct tggaacgtca agtatttaat cgaacgaaaa
     3781 ctacgaccca ttctccctta tatcagtttc gcacaactac tcgagatggc tataaaaaat
     3841 atgtatctaa atcttttatt tgtgaaagtt gtccattatt gaaaaattgt acagaaagcc
     3901 aaaaacacca aaaagaaata catcgacata tttgggaaga ctacttagat gaagcagaac
     3961 atttacgcca tacggtgtac aatcgtgaaa aatataaaaa aagaaaagaa acgattgaac
     4021 gcatttttgc agacgcaaag gaaaaacatg gaatgcgcta caccaagtat cgaggcttgg
     4081 aaaaaatcag gtgcatacaa tgcttgtttt tgtcgcaatg aatctgaaaa aattagctac
     4141 ttggcagtgg gagcatatgt cgttatatct tttattatac aaaaatagac aaaagtatca
     4201 gtctacactc ttagtaaaga ggaaactgat gcttttgtct acagtcttag agagtgggac
     4261 aatagttttt tcttctttac gtcttttcct ctccatgtat tttcctaatt ttcttaattt
     4321 aacgccatga gtgccaagcc aatgtcaatc ttggctcctt gtttccttca taagtggaaa
     4381 tccgtttccg atagatcgaa ccttcttttt cctctaaaag tttttttaca ttctgctttg
     4441 aaataacgcc atgtattatt cactaaaatt tgtcggtttc gctcactttt tgctttcgta
     4501 cattgactac gaacaggtca gcaccgacca ctttcacatt cgtaaatttt gaagacacgt
     4561 ataaatccac ttttatcttt tcttcgacta aagcgttgac cgcaaaagcg acatctgttt
     4621 cagaaatata ttgttccata tctaatggta aaattttttg gttgatggta taatttttaa
     4681 acataaagca ctcgcttcct ttctgattgg gtttggtcac tttaatccta cagggatgag
     4741 tgctttttgt gtaccttaaa atcagtaaat atccatccgt gaaaacgggt ggctttttaa
     4801 cagccctaga agggctcacc actcacagcc ctatctctct ttaactattt aaatggatct
     4861 acatactctt gtttacttat actgtctcta attcgatcat ctgcttcttg ttctcaaata
     4921 tatatttcaa ttgttttttg atttaaccca accgtactca cgtagtatcc tttggtccaa
     4981 aagcttttat tcccatactt atatttcaaa tttgcatgtt tttcatggat taaggttgca
     5041 cttcgtcctt ttaagtagcc cataaaacta gaaatactaa tctttggcgg tattcttact
     5101 aacatatgaa tgtgatcagg cattgcatga gcttcaatga tttcaacatc tttcctctca
     5161 cacaattttc ttaatatctg gccaatactt actcttaact ttccataaat cacttttcgt
     5221 cgatacttcg gggtaaagac tatgtgatac ttacaatccc atctcgtatg tgctaaactt
     5281 ttatcgctaa agctatttcg tccacgcgta gaaacgcgcg tttttttgtt tcgtacgcta
     5341 cgccaacgaa actgactaaa gtcaataaaa aaagtggggc aataatcaga tttttttctg
     5401 actattgtcc cactctcttt ttattactct tcatcttcta ctattccact aattaattag
     5461 ttgacagata caatgctttc acatcctgca cgaacaaact cgggctctga accaatcact
     5521 tgcgagttga taaattcaat gaccatagga agattaatcc gacataaagt ttaatctgtt
     5581 gtccttccat tatcagttta gaaatgatat tagtaggtgt accgcctaaa agatcgcata
     5641 gtaccacaaa atcatttaac gaatctatga cagtaagagc ttttgtctgt atgtttcagt
     5701 gccatcttct ggtaataaag gtaacgtgta gatattctct tgaggtccca taatcatttt
     5761 tgtacttttt ttcaattcct cacaaaattt tccgtgacta atcaatatca attttctact
     5821 catttttggc tctcccttat ttcgaaatca cacacaatgc agataaagcc acacacaagt
     5881 aatacgataa aaatcgctcg agttgggtca tatttttctt tcctaacaac caataaacac
     5941 ctcgacgatc ccagcgagta agattttaga taatatcata tcgatatagt tttgcatatt
     6001 caatgtcaca tcgccaatcg ttggtaccat aaaaatttaa tatcaatcat tgttgcaact
     6061 aatgctccta ccatgaacac tcttaataaa gtcgctacgt ccgttaatac agttaagcga
     6121 gattacacta gatgatctgt tcctgccaag gcaagagcca gaagcggttg cctgcccatt
     6181 tgcctttggt gtgtcggagc tgtccgataa agttcaccgc ccgatccgct ctggctttgt
     6241 cgtagtgaca ttccgaaagc atgaatcggc ttggtgtgta ttcctttaat ttgggatagc
     6301 cctttggtct ggtttctgcc atcagctacc acctcccaac agagcctcca tcgcatcttc
     6361 tgctccgaca ccaccgccat caccggcaat gatacggctt ctggacgaag gcgtcagacc
     6421 gaactgctcg gaaaatcggt tcataatttt cagataggtc tgggcaataa acacctgtgg
     6481 tacctgctgc caatagccgg atagggtttt cacgatgata ccgtgctggg taatgaattc
     6541 ctcggcttcc ttccagcggg cgtaggcttg gcactaaccg gcaaaggcag ccatatccac
     6601 atctgtcaag attccgatgg cctccatctg ctttgccaga cgcttccatt ccttctttgc
     6661 ctctggttcc agccacttcg gacaggtcgg tgccttcttt tccggcgttg actcttttgc
     6721 attgagtggg cgattgcccg gatttccctc cagcttcttc atggctgtcg gggttgattt
     6781 tcttcctctg gttgccacag tcgtcacctc ccttcaatgt atttctgcca gtagacagag
     6841 aacagagcca agtcgaaacc tgactctctt ctctatctgc taacttgtat ttcagtccac
     6901 tcttctgatg aatcgcaccc gcaggatttt caagatgccg caggcaaatt ccaagaagtt
     6961 tctgaccgca tcataaccat cgtagccgtg tagccacatc cttccacctc gccgccgtcc
     7021 tcgtcgctga tataaatgat tctgtattcg ctcatctgtt gttttctcca gtcttttgtt
     7081 tttttgtagt acatatatca ctctaaaagt gattattatc aagtcgtttg aaagccataa
     7141 tgtacacaat cattcgactg gtttttggtt cataattgac cgccaaagtg agcaaaagaa
     7201 agggacctct gtctgtttca gaagtccctt tcggtatttg gttcttagtg gttcaggctg
     7261 tattctcaaa cctcatactc atcttcgagg tctgcctctg caatgatctg gccgatgctg
     7321 tcgtaacgct ggacctgcat ctggatggca tccttgtctg ctccctcgtc catcatcttc
     7381 agcagcttgt ctgattcctt tttgtaggcc cagaaaagga tctcctgaag gctggtctcc
     7441 tgctttttct tgcgaagggc gaccttcttc tcttccaccg cctcgttcaa cttggtgaac
     7501 tctgcccaag caaggatacc gtctcgcttg attgcgatct ccacatcctt cagctccggg
     7561 atggtctttg cacccttgat ctccttcaaa tatttctttg ccttttcgct cattgccatt
     7621 agcgtttcct ccttgttttt gggattttcc cctttggttg tgtgtatatt gccatagtct
     7681 ctcgactata tcaagtcaat ccacaggcat atactacaca atcatttgga gctgtatttg
     7741 tatacattat gccgatttat gagagaagaa aaggctccag ccgttcgact ggaaccttgg
     7801 gggggtcggt ttcttaggcg gcgtggcggt gtagaccttg aggtagctga tgttcttcca
     7861 gacgtccgtg cccttcagtc tgaccatgcc gtccagctct cggtcgaggg tgaacttgcg
     7921 gaaaagcttg atgcggttga ggctgtttgc tagctccttg ccgttcttgg catccagaac
     7981 tgcgtcctcg atctccgcaa tactaatttc ctgtctaaac caaaggttgc tcaaggcttc
     8041 gggaatgccg tttgcggtct gcagggggtt gaaagtttcc aatacagcag gggtcgaaac
     8101 cacctgtccg agctggaatt ttgcgttccc taaaatcatc tgcataattt cttccatgtg
     8161 aatcatccat ttctgccgtt gtggtattcc ctttggcagt acagtactat atatcactct
     8221 gaatcgtaca tttatcaaga agcggatttt tgtggaatca ccaattcgat ataatagtaa
     8281 ttgcatgaaa aatctaagca ctggattggt aggtgatgat tatacgtgac gatcacattt
     8341 acacctgtga ttcctgccac tacagcttcc ctgccgacga acagccagag cgttgtcctg
     8401 actgcgaaaa gacagcaact cggctggata ccgaaatcga aactgaagat tactatcgtg
     8461 ttcgtgctga aatcaaagct gagattaaag ctttgaatgc gggttaacga caggtactga
     8521 ccggcggctc aacaccgccg gctttttatt accacatctt gcggaattcg ctgggacgaa
     8581 caccttccac cttgaaaaaa gttctggaaa aatagtttgc atctgttatg ccaatttttg
     8641 ttgcaatctc ttccatactt agatttgtaa agcgtagcaa gtgcttggcc tgtgtgattc
     8701 gtttctgatt cagataaccg acaacagttg taccgtaagt ttcacgaaaa atcttagcta
     8761 agtactgttt attgataaaa aactctgcac tcaactgatc cagcgtaatc ttctccgtgt
     8821 aatggtcatc cagatattgt tttatgttct gcaattccat tttttttgta gagaaattcg
     8881 gcactttgtc aggattccaa gaatcttcca tcaaatgcat cagcagttca ctgagtagaa
     8941 cattgatttt catatcccgg acatactcct gtccctctgc caccatatga aggctcctca
     9001 gcaagtcagc atacttatct gtttttgatg ttttaaagac cggttgacca cctcgttcaa
     9061 ggtatttttt gtagacttcc gtcataccaa tgccattaaa atggcaccac cgcaaagtcc
     9121 acaaatcatc ttctgatgac tcatggacat acggcttcaa acagtcaata aacaccatat
     9181 ctcctacttg aagcgtatat ttctgcccct tatactccaa tgtgcctgaa ccaacttcta
     9241 ccaggaaaca aaggtaagat gacaatcctg tacgcctaga tgtatgtgcc ttcagagcag
     9301 tcagctgtcc aatttcctgc aaatacaaaa gagaggatct tgcaaagaac gatgccgtgt
     9361 acagcacacg atttgacttc accgatttca aagcgttaga aaacagttcc tccattacaa
     9421 gatttctcct ttcttttctt actcgaacat ttccgccagt tcagcagcca taatagcttc
     9481 catagcttcg gcggcttcct tgttctctgc gctgacgctg atataggtct tcagcttcgg
     9541 ctcagtacca gaaggacgaa ccacaacaga gcagttatct tccagcataa acttcaaaac
     9601 atcagacttc ggcagaccat tcaggccctt actgtagtct aaagtttcaa taaccttctt
     9661 accaccaatt atatcgagac ccttgtggaa ttcaaccata atttcctgca tcttggtaaa
     9721 acctgccgca ccgtcaaact cgaaggaatg cagggtgttc aaacaatagc catactttgt
     9781 ataaatctcg ttcagcttat ccagcaggct gataccctga gtcttgtagt aagagaacat
     9841 ttcacagatt agataagcag cgttaacacc gtccttgtca cggacatagc taccggacag
     9901 atagccgtaa gattcctcaa agccgaagat atagctgtca accttaccag ccttttccaa
     9961 cataccaatc tgctcaccga taaacttaaa gccggtcagc acatcgatgg tacgaacacc
    10021 atagttggat gcgatacgtt cacccaaatc catggtgacg atggtcttga ccatcacagg
    10081 gtcagcaggc atttttccat gcttaattct ctgagaacag atgtagtcca gaagtagcag
    10141 accagtctca ttaccagaca acagaacata gtcaccatgt gcgctcttaa ctgcgatacc
    10201 aacacggtcg cagtcagggt cagtggccaa cagcagatcc gcattgtact tcttggcata
    10261 ctccataccc agagacatag cttccttaat ttcagggttc ggataaggac aagttgggaa
    10321 attaccgtcc ggcagacgct gttcttccac aacggtcaca ttgctgtatc ccatctctgc
    10381 cagggtgcgg gtaacaggca ccagaccggt accattcaga ggagaataca cgatagcaac
    10441 attcttgtcc acttcttcgc caaacaggac ggactgcccc ttcacttctt cgatgaaagc
    10501 agtcagaact tccgactgaa tggatgcaat cttagaagca tcgccatcca tctttacatc
    10561 agcaaagatg tccagctttt cgatttctgc cagaattgca gtagcagcct cggtagtaat
    10621 ctgacagccg tctgcaccgt ataccttgta gccgttatat ttagaagggt tatgagatgc
    10681 cgtaatcata acaccggcat ctgccttcag ataacgagtc gcaaaagata ctgtaggaac
    10741 aggaagcagt tctggccaca agtgcacctg aataccattt gcagcaaaga tgtctgcggc
    10801 agtctttgca aacacatcag atttgatacg ggaatcatag ccaaccacca cggaagcggc
    10861 ttcatagttc ttattcagat agtctgccag accttggctt gccttaccta cggtgtgtac
    10921 gttcatgcga ttagtgcctg caccgatggt accacgcaga ccaccagtac cgaaagacaa
    10981 atttttatag aaagagtcct caatgtcctt ctcagaatag gaacgcagtt cttctataat
    11041 atctgcattg gcatgattca gccaacgctc atattctact ttataattca tattattatc
    11101 ctctcacatc cagaaactga atcgacccgt gaatgctgat ttcagtagaa tcggccggca
    11161 caaaaaggca gtccccctta tagatgtcca acgattcatt atcaaatcga acggtaccac
    11221 agccattgac acacagcaac acacggaagc tgattgaatc cgcacgatag tgtaccgtct
    11281 gtcttctctc agtgttgaca atcatacgag agacctcgaa atgcttgcat ctgcaaagca
    11341 attccgcagc tacaccagca cgatatttta gaacacgaag cggctgtttc ggttctgaca
    11401 accctttcaa gttcgctact tccagcgctt tgtctacatg aagttctctc tttttaccat
    11461 ttttgtcaac acggtcataa tcgtagagac gataagtcag attggaattt tcctgaattt
    11521 ctgcaattag tgcacccgca ccaatcgcat gaatcgttcc cgcctcaata aagaacacat
    11581 cattcttttt gaccggaacc ttctgaagat aacgctcaac agttccctga gcaatcgctt
    11641 cacggatcac agcttcttct gttattttct gcagcccata gattaagctg gcatccagta
    11701 cataccacat ctctgtcttt ccaagctgcc cgttttcttt ttcaattgca tattcatctg
    11761 tgggatgtac ctgaacagac aaatcctttt tcgcatcaat gaatttgatc aggatcggca
    11821 attctccttt ggtctgcgga tgcgttccca gaaactctgg gtgttcttgc aacacctccg
    11881 tcaaggtcat cccctgatac tctgaccaga cacaacaaag ctcggtccat ccgggtgcgt
    11941 agagcattcc aagtttccgc aagaggagac atctcaattc cttttgcaaa ttcatcattc
    12001 aggcggttcc cccccacaga taatcctttg cggaaggctt caataaaaac ggtttctgta
    12061 taacgagccc tcctccgttt tttaatactc taactcaaat ttctgtttat ctttaacatt
    12121 gatatccttg ccaagctgta cttcaatcag cttcagctca gtatctgcca taatggtatg
    12181 ccgacagcca gcactcatgg tgatcacatc gccagcctta acaacctgct catatccatc
    12241 cacgatggtc ttaccagtac cggccagaac cacccacacc tcgtcacggt tcttgtggct
    12301 gtgataattc atggagtgac tagcattcag agtaacctta atggtcatgc tctctgcttc
    12361 tacatccata accttataac taccccaaga cttctctgcg aacatgatct gctgctcgaa
    12421 cttgtcaacg aaaggcttaa tatagctgga ctgttcctta tcggaaacca gaataccttc
    12481 ccgagatgca cagatgacca catcatgcag acccatagcc aacacaggaa catccagttc
    12541 gttcaggatg tgtacgccat cgcaattctc attcataaca cctttaccga taatggattc
    12601 ctccatacct tcggtcagag tattccaagt ccccagatcc ttccacatac cagcaaaacg
    12661 ctgtacctga atcttgtcct catgctcaac aacggcatag tcgaaggaaa tcttagtcag
    12721 agtatcgtat ttgttgaaca gatcctgata atctacaaag tcaatcagct gatgtgcacg
    12781 ctccatgaca tacttcagct tgtagacaaa cacaccaccg ttccacagtg caccctgtgc
    12841 gatatattcc tttgccacag cctcagaagg tttttccttg aaggtattta cgaaagcagt
    12901 ctgctctgcg ctctcaggaa tgatgtagcc gtacttctcg gaaggataag tcggttcaat
    12961 acccatgagc accagattag cttcgccttt gtcagcctga tcggacagag atttcagggc
    13021 ctcaaaatag tcatcttcta cgtaagggtc aaccgggcaa acaactacgg actcttctgg
    13081 atcaatgccc atcacatcta ccagataagc ggtagccaat gcgattgccg ggaaggtatc
    13141 gcggcgacaa ggctctacag atacacccac atcctcacca agttggttat ggatagcaga
    13201 aacctgtgtc ttagaggttg caatcgtaac agttgcgtcg ttatctacct tcttaatctg
    13261 gcggtagaca cgctgaacca tggattcata tccatcttca gttttgaaaa tcttaatgaa
    13321 ctgcttggaa cggatgtcat tagacagcgg ccaaaggcgc ttaccagaac cgccagacag
    13381 taaaacaata ttcataatga tctctccttt ttataagaaa gaggctgctg caatcctcaa
    13441 aaatcacagc aacctcggta aattagcgtt cagcacgcat cgggttattc aggaagtcct
    13501 cgtaggacag acggatgcca tcttccaact cagtcttata tgtccatccc agcttggttg
    13561 ccttggaaac atccagcagc ttacgaggtg taccgttcgg cttgctggta tcccacagaa
    13621 tctcgccttc gtaaccaata acctttgcta ccatctcggt cagttccttg atagacagtt
    13681 ccttgccagt acctgcatta acagtctcgt caccgctata gttattcatc aggaacacac
    13741 acaggtttgc cagatcgtca acatatagga attcgcgcag aggagaacca tcaccccagc
    13801 aggtaacagt aggcagacct gcttccttag cctcatggaa gcgacgaatc aaagcgggga
    13861 gcacatgact gtgagtagga tgatagttgt cgttaggtcc atacaggtta gtaggcataa
    13921 cagagatata gtcagtaccg tactgcttgt ttagaaactc acagtacttc aggccagaaa
    13981 tctttgccag tgcataagcc tcgttggtct tttccagctc acttgtcagc agacagcttt
    14041 ccggcatcgg ctgcggtgcc atacgcggat agatacagga agaaccgagg aactgtaact
    14101 tcttacagcc attctcccat gcggagttga tgacgttcat ttccagaatc atgttttcat
    14161 acatgaagtc tgccagagcg ttctggtttg cgataatacc gccaaccttt gcagcagcga
    14221 gaaatacata atccggcttt tcctgtgcga aaaatgcttc aacagcgtcc tgacggcaca
    14281 ggtcaagctc cttatgggtg cgggtgataa tattgttgta gtcctgacgg ttcagctcgc
    14341 gcacgattgc ggaaccagcc atgccacgat gaccggctac gtagattttt gcgtcttttt
    14401 ccatcataat aatgcttgct cctttctaaa tttagagcat ctctcattgc atgagagaaa
    14461 tcaatttgtc tgcgatttcg ctcgcagcat cataaaatgt atcaaaattt gaggtgtcta
    14521 gctgtttgag gtcctcaatc acccgctcta cgcggtggag gttttccggt gtgacggaaa
    14581 ttgccagacc cagtttttgc aatttacaaa tagttgttct atcttccaaa actgtatcgc
    14641 gggcgaacag tgccatcgga cgctgtgcga gaatacactc cgcaacagtt ccccatccag
    14701 cctttgtaat aacgtaatct gccgccgcta tatagtcctg tgtattgggg gtgctgttag
    14761 aaataacggt tacgttttct cccacaagcg gaacacctag cgttgtaatg aaatcatacg
    14821 gcgcgtgact gacatcaatc ggctgtttga accgcgcgga cataccgagt gcgacaaata
    14881 caggcggttt ttcatgctgt gatttaatgc gttgcacttc gtcgttatga atcggacgcg
    14941 ccacaacgga agtcggtgtt ttttcgctgt tttggagaaa ttccaacatt tcttcgttat
    15001 ttagcgcata aagcatggtg tgctgaattt taccgtaacg cttggcatat tctttccaaa
    15061 tatgttcggg taaaaattcg cgatacagct ctgtccaagt gaagtttcct acatacagga
    15121 aaggtactcc tgccatatcg caggcatcca ttgcccagat tggcatatcg caaagcactg
    15181 cgctgacagc ttctcgcttg agccattcgg cttctgtctt tgccaaagaa ggaacctttg
    15241 ccaaaaaata ttcacagatc tcggtcagct tacccgtgtc caccagtagg gtgccctcac
    15301 ggttaatcag accgatatct gtgtttattg gacgatagcg cacgcggtct atcagctctt
    15361 tgggcagttc ctgctccaaa ttgcgctttg caaaatccag atgatttgcg ccgcaaacca
    15421 gtgtaatctt cgcttcgcat cggcagagtg ttgccataat aatgggcaga ttccgcgcga
    15481 tatgtccgaa gccgtgactg gaaagataga aacagatatg tttattcaat gtcagcttac
    15541 ttgactgcgt tcttcattgc agcttcgcgc tttgccaatt cacggtcatg ctttgccatg
    15601 atgttaacca gttcctcgta agaggtcttc tgcggattcc aaccgagaac ggtctttgcc
    15661 ttagtcgggt cgccccacag gttgtcaacg tcagtcggac ggaaccactg tggatttaca
    15721 caaacgagca tcttgccggt ttcaacatcg tagccctttt catcaatgcc agtgccttcc
    15781 caacgaatgg tcatgccatt tgctgcgaat gccttctcgg tgaagtcacg aacagtgtgc
    15841 tgctcgccgg tagcgatcac gaagtcctcg ggagtctcat gctggagaat catccacatg
    15901 cactcaacat agtcctttgc atagccccag tcacgcaggg agtccatgtt gcccagttcc
    15961 agatggtcct gcaggccttc tgcaatgcgg ccggcagcga gggtgatctt acgggtgacg
    16021 aaattctcac cgcgacgttc ggattcatgg ttgaacagaa taccgttgac agcgaacatg
    16081 ccataagctt cacgatattc cttagtgatc cagaagccat actgctttgc aacagcataa
    16141 gggctgtaag gatgaaacgg agtggtttca cgctgaggaa cttcttcaac cttgccatac
    16201 agctcagaag tagatgcctg ataaatctta gtcttcttcg tcaggcccag aatacggcaa
    16261 gcttccagaa tacgcataac gcccaaagca tcaacatcgc cggagtactc aggaacatca
    16321 aaagaaacct gaacatggct ctgtgcagcg aggttataga tttcatcagg ctgaacaatg
    16381 ctgatgatac gaatcagaga agatgagtcg gacaggtcac cgtcatgaag ggtgatcttg
    16441 cccatcaaat ggtcaatgcg agcggtattg gaaatagaag cacggcgagt gataccgtga
    16501 acttcgtagc ccttctctag caggaaatct gccagatagg aaccgtcctg accagtaata
    16561 ccagtaatca gcgctttttt catgatacat tatcctttcc tatgtaagtg ataaggccat
    16621 ataaatggct cgtaatttct aatacttttc aagaatacac gataattttc gatatttcat
    16681 agaacaatct acgcacgcat tagcacaata ttagtttttc gtg
//
LOCUS       DAZKLD010000056        16591 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.056, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000056 DAZKLD010000000
VERSION     DAZKLD010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16591
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.056"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..173
                     /locus_tag="ACG8KR_001488"
     CDS             <1..173
                     /locus_tag="ACG8KR_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HFQ0200857.1"
                     /translation="NKQIKRYSRRKEQFQNEESLERFLVSIFDTYNQKFLNRSHKGFQ
                     QVTDTLVSMFTE"
     gene            complement(376..1032)
                     /locus_tag="ACG8KR_001489"
     CDS             complement(376..1032)
                     /locus_tag="ACG8KR_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFQ0200858.1"
                     /translation="MDSRVLKEYLDKNDFPIIRKGYHKYLTFEGMEDSHTYILKEGTV
                     KASVISNDGREFNLRYIKDLEIVSLLKDEYSQFIDSPYNIRVESEYAEFYRINRVRFW
                     QNINQDPKLQHYVKEYYRYRLLYSMKKMQQMLLNGKLGAVCTQLYELYDLFGVRTEEG
                     MLIDFSITNEEIAKFCGISTASSVNRILKQLKKDGIITVKDHHIVILNMDLLEDNIIF
                     "
     gene            1225..1503
                     /locus_tag="ACG8KR_001490"
     CDS             1225..1503
                     /locus_tag="ACG8KR_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0200859.1"
                     /translation="MNYWMNTIINRLETAYQTRFDMKASLVFLNDAYQNSIELIKAVD
                     ENPTNECEEFLNLFMSTRDLFIRQLVDRYPSNYHDVEVQIQKLKAYSA"
     gene            1522..2730
                     /locus_tag="ACG8KR_001491"
     CDS             1522..2730
                     /locus_tag="ACG8KR_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFQ0200860.1"
                     /translation="MEKKLFNKYFIGITLINFIVYLIYYLLMVIMAVIAQDTLHASLS
                     QAGFASGIYIIGTLLARLFMGKQLELFGRKFTLRGGAIFYLLSTLAYLYTPTIGALYL
                     VRFLNGFGYGTVSTATNAIVTAYIPASKKGEGINYYGLSTSLAAAVGPFLGMILLNMT
                     DFRFIIWFSIVLVFFVTIACLVFPVKNIPLSEEQKISLRSWNINSFIEKKALFITGIA
                     FLMGLSYSSVLSFLSSYTKVIHLVAAGSFFFVVYALVITFTRPLTGRIFDVKGEQYVM
                     YPSYLFLTAGLFLLSVTTNSVTLLISGALVGLGYGTFMSNGQAVCLKIVREHRISIAL
                     STYFIGLDLGLGVGPYLMGMLKSLTSFRGLYVIAGVIPLICTALYLVNGRKHSHEAEG
                     ELIESLKNEI"
     gene            complement(2878..3243)
                     /locus_tag="ACG8KR_001492"
     CDS             complement(2878..3243)
                     /locus_tag="ACG8KR_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2512 family protein"
                     /protein_id="HFQ0200861.1"
                     /translation="MKHVKALVVKAIMIWAILWVVLTGLYGVSFMDSTIVGVIIVVMI
                     YVLGDLLILRKVGNIAATIADAGSAAVILWLYLYFMNDTVDIWMKVLIPALLIGVAEW
                     FFHKWLLSQGIVPDERKME"
     gene            complement(3390..4163)
                     /locus_tag="ACG8KR_001493"
     CDS             complement(3390..4163)
                     /locus_tag="ACG8KR_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFQ0200862.1"
                     /translation="MLNEVENRQQEIVNYLKNNQFARVTDLIELVNYSEATIKRDLVF
                     LEKSGLIRRVRGGAMLIDNRKIDVPYLMKITKLEEETEKRKIADTASSLIRDDMVLFL
                     DSSTTSLHLVRNLARFDGLQIITNGIVTAAMLSEYTTAKVSVLGGAIVPKRATINGSK
                     AYNDALSYYADLAIMSCRGLDFSQGVTETHEGEAMVKKAFRKQSDRLAVLVTQQKIAH
                     KFMHQAIAFHEIDYLVTSKELTKEQYAKVKEHHIECLYG"
     gene            complement(4337..5197)
                     /locus_tag="ACG8KR_001494"
     CDS             complement(4337..5197)
                     /locus_tag="ACG8KR_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HFQ0200863.1"
                     /translation="MLYTMKELLKVAKENQFAVPAFNICSYDMMKAIIEEAERLEAPV
                     ILEIHPDEIAYLSDEFVAAVRAYAYTSKLPIVIHLDHGGTIEDVLRAIRNGYSSVMID
                     ASLKSFDDNVQITKKVVALAHAVNVSVEAELGTIGNNGSAEGGAETIIYTDPDQAKIF
                     VKETGIDTLAVAIGTAHGLYPKNRTPKLNIDLLKELNQQIDLPFVLHGGSGNPDAEVS
                     EAVKYGVAKVNLSSDLKSVFFNALREILSENPSMYEPNAIYPAANKKVQEVVKHKMEI
                     LNTVGKASLY"
     gene            complement(5197..6615)
                     /locus_tag="ACG8KR_001495"
     CDS             complement(5197..6615)
                     /locus_tag="ACG8KR_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFQ0200864.1"
                     /translation="MKIVGVTACPTGIAHTYMSAEKLEVTARNLNHQAKFETQGVKTE
                     NQLSEQEIKEADAIILAVDKEIELDRFAGKKVKRVSTSRAIKEPQVVIDEALRDIGVF
                     VVSNEKEPAANEKKPTIYNHFMNGVNYMLPFVIAGGIIIALSFAFGITAADPNSADYN
                     VLAAAFSRIGGDTAFAMMVPALGAGIATSIAGKAGFAPGIVAGLLASTGGSGFLGGMI
                     GGVLAGYICDFLANKISVKKEFSAIYQLIVVPFLSILIIGLLMVFVIEQPITWVLDAL
                     TNWLNSLGNTSGLLFGLLIGIMMAADMGGPINKSTSTFSIGLMSAGVNAPIAACMAAG
                     MTPPLGIALATLLFKHKFTKEERTSGQSCWILGASYITEGAIPFAVSDPIRVIPSLMT
                     GSAVAAGISMAVGVTSMAPHGGIWVMLIPNVMNNLPMYVLAIASGTVTTAFMLGLLKK
                     TVKEKKQDKWTKNENLTNEVEA"
     gene            complement(6672..7121)
                     /locus_tag="ACG8KR_001496"
     CDS             complement(6672..7121)
                     /locus_tag="ACG8KR_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFQ0200865.1"
                     /translation="MRFDQLIAEELIVFDPSIDSKGSLFERIGDVLKKTNKVRNVQKV
                     VKALFKREEEISTGIEDGFGIPHAKSKYVTKPALCFFHTAKLTDYSGIDEMPIEYIFA
                     IVVPEKSADMHLEILSTLSRRLMNADFRKGIKEATTPADIIRQISQE"
     gene            complement(7566..7829)
                     /locus_tag="ACG8KR_001497"
     CDS             complement(7566..7829)
                     /locus_tag="ACG8KR_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HFQ0200866.1"
                     /translation="MKENIHPDYHPVVFMDSTTGFKFLSGSTKTSQETVEWEDGNTYP
                     VIRVEVTSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAE"
     gene            complement(7928..9223)
                     /gene="rho"
                     /locus_tag="ACG8KR_001498"
     CDS             complement(7928..9223)
                     /gene="rho"
                     /locus_tag="ACG8KR_001498"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HFQ0200867.1"
                     /translation="MSDYLTMAELENKTLKEVYSYAKEFKIPYYSKMNKKELSLAVIR
                     AQAEKQGFYYMEGILDIVGQDGYGFLRPINYGPSAEDIYISASQIRRFSLRNGDKVAG
                     KARPPKESERYYGLMHVESVNGKDPEEAKERPHFPALTPLYPQRQLQLETTPQRLSTR
                     MIDIFSPIGFGQRGLIVAPPKAGKTSVLKEIANGITENYPEVELIVLLIDERPEEVTD
                     LERSVKGDVVSSTFDLQPQNHTRVSELVLERAMRLVEDKRDVVILMDSITRLARAYNL
                     VVPPSGRTLSGGIDPAALYKPKKFFGAARNIEEGGSLTILATALVDTGSRMDDVIYEE
                     FKGTGNLELQLSRELSERRIFPAIDIKKSGTRKEELLMPSEELEEIWKLRKNMTGDSL
                     EYTEQLIRFLRKTKNNQQFFKEFQDVSFGKKNPTRNLKR"
     gene            complement(9220..10491)
                     /locus_tag="ACG8KR_001499"
     CDS             complement(9220..10491)
                     /locus_tag="ACG8KR_001499"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFQ0200868.1"
                     /translation="MKKIVIKGNRPLAGEVTISGAKNSAVALIPAAILADSPVILEGV
                     PDIQDVHSLIEILEIMGATVHFSNNILEIDPREVVSIPMPHGKINSLRASYYFMGTLL
                     GKFGEAVVGLPGGCYLGPRPIDLHVKGFEALGAEVTNEHGAMYLRTENKGLRGNRIFM
                     DVVSVGATINVMLAAVKAKGQTVIENAAREPEIIDVATLLNNMGAKVRGAGTDVIRIE
                     GVETLHGCRHFMIPDRIEAGTYLALAAAVGNGIKVKNVIFEHLESFIAKLQEIGVHMK
                     ISEDEIEVYPSKELKPANIMTYPYPGFATDLQQPLTALLLMTTGTSEIIDTIYAKRVN
                     HVPELARMGADITVEGNMIIVNGPNKLHGTEVVASDLRAGACLVIAGLLAEGTTTIYN
                     VDYILRGYDHIIEKLTALGASIEMIEEVGAE"
     gene            complement(10656..11525)
                     /locus_tag="ACG8KR_001500"
     CDS             complement(10656..11525)
                     /locus_tag="ACG8KR_001500"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HFQ0200869.1"
                     /translation="MPVVSGAEFLKAARKGGYAVGGFNTNNLEWTQAILEAAEAKQAP
                     VLIQTSMGAAKYMGGYKICKDLITDLVEAMNITVPVAIHLDHGDYEAALECIELGYTS
                     VMFDGSHLPFEENLKLAKDVVEKAHAKGISVECEVGSIGGEEDGIIGNGELADIEECK
                     QMVATGIDYLACGIGNIHGQYPENWKGLAFDHLQAIADAVGPDMPLVLHGGSGIPQDQ
                     IEKAISMGISKVNVNTEFQLSFAAATRKYIEAGKDKEGKGFDPRKLLAPGKAAIVEDA
                     KSHIDWFGSANKA"
     gene            11628..11840
                     /locus_tag="ACG8KR_001501"
     CDS             11628..11840
                     /locus_tag="ACG8KR_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0200870.1"
                     /translation="MKMKIFKKFFAKNKISNSALIEKSLLLSIFFPTNNNKSEKEKSD
                     KKIAYPLLLLLFSFYQVQKLFCFVIC"
     gene            complement(11817..13427)
                     /locus_tag="ACG8KR_001502"
     CDS             complement(11817..13427)
                     /locus_tag="ACG8KR_001502"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HFQ0200871.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITNEIKEKIMRAAKMTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGTDNVMYIHTTLIPYLKAAGEMKTKPTQHSVKELRGLGIQPNILVVRTEQPVS
                     QSVKNKLAQFCDVEPEAVIESPDVDTLYSIPLVLQAQGMDQIVCDHLKLDTPEADMTE
                     WKELEERVLNLKKKVRIALVGKYVELPDAYISVVEALKHSGFAYNSDIEIDWIKAQEL
                     TRENVEERLKDADGILVPGGFGDRGVEGKIEAIRYARENDVPFLGICLGMQMACVEFA
                     RNVVGLEDASSAETNPDTANNIIDLMADQENIENLGGTLRLGLYPCKIKKGTKTAEAY
                     DGADVVQERHRHRYEFNNKYRQLFEEQGLVFSGVSPDNRLVEIVELPEKKIFVGCQFH
                     PELISRPNRPQKLIKAFVGASLADHETK"
     gene            complement(13649..13894)
                     /locus_tag="ACG8KR_001503"
     CDS             complement(13649..13894)
                     /locus_tag="ACG8KR_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0200872.1"
                     /translation="MKEKDCFYDKLYKLLLQVGKSSSLLGGILSIIMFVLFFYLLFHL
                     KFFSNDFLICLGLTLAMFISSLLCGLNFFCTHKGLKK"
     gene            complement(14620..15960)
                     /gene="glnA"
                     /locus_tag="ACG8KR_001504"
     CDS             complement(14620..15960)
                     /gene="glnA"
                     /locus_tag="ACG8KR_001504"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HFQ0200873.1"
                     /translation="MVKKQITGEEIKRIIEEENVRFLRLMFTDILGTIKNVEVPVSQI
                     DKVLENKMMFDGSSIEGFVRIEESDMYLYPDLSTWMIFPWESAHGKVARLICDIYNPD
                     GTPFAGDPRGNLKRALKDMRDLGFTSFNLGPEPEFFLFKLDEDGGITTTLNDKGGYFD
                     FAPTDLGENCRRDIVLELESLGFEVEASHHEVAPGQHEIDFKYADVVDACDNIQTFKL
                     VVKTIARKHGLHATFMPKPLFGINGSGMHCNMSLFNDEGNVFYDKDGELELSETAYHF
                     LGGLLKHARAYTAVCNPTVNSYKRLVPGYEAPVYVAWSGRNRSPLVRVPESRGLSTRL
                     ELRSVDPSANPYLAMAVLLQAGLDGIRNQITPPAAVDRNIYVMDEEEREAAHIQDLPS
                     TIHNAIKELRKDNVMIDALGQHIFSNFVEAKRLEWAAFRQTVSEWEREQYLELY"
     gene            complement(15988..16380)
                     /locus_tag="ACG8KR_001505"
     CDS             complement(15988..16380)
                     /locus_tag="ACG8KR_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFQ0200874.1"
                     /translation="MGEKELRRSMSVFPIGTVMKLTDLSARQIRYYEEQDLVHPERSE
                     GNRRMYSLNDIDMLLEIKDYLSEGLNMAGIKRVYEMAQAKKQEAKNKKPLTDHDVRQI
                     FRDEILAQGGLSKTGHYQSQGLQRQFFD"
BASE COUNT         4758 a         3509 c         2818 g         5506 t
ORIGIN      
        1 tcaataagca aattaaaaga tacagccgta gaaaagagca gtttcaaaat gaagaatcac
       61 tagaacgctt tctagtcagc atttttgata catacaatca aaaatttcta aacagaagcc
      121 ataaaggttt tcaacaggta accgatacat tagtttcaat gtttactgag taactaatta
      181 ttttgcagga ggacaattta tttacacaaa attattgacg ctcccaaacg gcaggctcac
      241 aacctcttgg taaacggtgt tcaatcagct gaccctcggt attagctcaa aaaacggcaa
      301 aatatgagga actatttttc cgtttgtttc tctaatactc aggtcaaaac gctgatttca
      361 caacctcttt gttcatcaaa agataatatt gtcttctaac aagtccatat tcaaaataac
      421 gatatgatgg tctttaacgg tgataatacc atcttttttc agttgtttta aaattctgtt
      481 tacactgcta gcagttgaga tgccacaaaa tttggcgatt tcttcattcg tgatcgaaaa
      541 atcgatcagc atcccttctt ctgtccgcac accgaataaa tcatataatt cgtaaagttg
      601 cgtacaaaca gctcccagct ttccgtttaa tagcatttgc tgcatttttt tcatcgaata
      661 aagcagacga tagcggtaat actcttttac atagtgttgt agctttgggt cttggttgat
      721 attttgccaa aagcgcacac gattgattcg ataaaactct gcatattctg attcaactcg
      781 aatattatac ggcgaatcga tgaattgtga atattcgtct tttaaaagag agacgatttc
      841 aagatctttg atatagcgaa ggttaaattc tctcccatca tttgaaataa cacttgcttt
      901 taccgttccc tctttcaaga tatacgtatg ggaatcttcc atcccttcga atgtcaaata
      961 tttatgatat cctttgcgta taatcggaaa atcattttta tccaaatatt cctttaagac
     1021 tctactatcc aatgatgctc ccccatccaa actttcatct aaaaagatca tacagtactt
     1081 taaaactttt taaagtccaa aggtatttta ctaatgatat taacatttgt taagtacctt
     1141 tttccatcac ttatgcgatt tttttccttt tttttattcg ttatactgca gaagtaaaat
     1201 tttttaagaa tagagggaaa aaacatgaat tactggatga acacaatcat taatcgatta
     1261 gaaacagcat atcaaacaag atttgatatg aaagcttctt tagtcttttt aaatgatgcg
     1321 tatcaaaact cgatcgaatt gatcaaagca gtggatgaga atccaacaaa cgaatgcgaa
     1381 gaatttttaa atctgtttat gtctacaaga gatttattta ttcgccaact tgttgatcgc
     1441 tatccatcca attatcacga tgtagaagtt caaatacaaa aattaaaagc atattcagct
     1501 taaggggaat gtataacata catggaaaag aaattattta ataaatattt tattggaatc
     1561 acattgatca attttattgt ctacctgatt tattatctgt tgatggtcat catggcagtc
     1621 attgcgcaag acaccttaca cgccagcttg agccaagcag gttttgcttc tgggatctat
     1681 atcatcggaa cattgctggc acgcttattc atggggaaac agttagaact atttggtcgg
     1741 aagtttacct taagaggcgg cgcgatcttc tatctccttt caacattagc atatctttat
     1801 acgccaacta tcggggcatt gtatttagtt cgattcttga atggattcgg ctacggtaca
     1861 gtttctacag ctacaaatgc tatcgttact gcatatatcc ctgcatctaa aaaaggggaa
     1921 ggcatcaatt attatggatt aagtactagt ttagctgccg ctgttgggcc atttttagga
     1981 atgattttgc tgaatatgac tgattttcgt ttcattatct ggttttcaat tgtactggtc
     2041 ttctttgtta cgattgcctg ccttgtcttt cctgtaaaaa atattccatt atctgaagaa
     2101 caaaaaatat ctcttcgctc atggaatatc aatagtttta ttgaaaagaa agcacttttc
     2161 attacaggaa ttgccttttt gatgggtctt tcttattcaa gtgttttatc atttttatct
     2221 tcttatacaa aagtaatcca cttagttgct gctgggtcat ttttcttcgt cgtatatgct
     2281 ttggtcatca catttacacg tccattgact ggaaggattt tcgatgtcaa aggagaacaa
     2341 tacgtgatgt atcctagcta tttattctta acagcaggat tatttttact gagtgtaacg
     2401 acgaatagtg tgacattgct tatctctgga gcattagtcg gcctaggtta tggtacgttt
     2461 atgtcaaatg gacaggctgt ttgtttaaaa atcgtccgcg aacatcgaat cagtattgct
     2521 ttatctacct actttattgg tttagaccta ggcttaggcg ttggtcctta tttgatgggg
     2581 atgctaaaat ctcttacttc atttagagga ttgtatgtga ttgccggagt cattccattg
     2641 atatgtacag cactctatct tgttaatggc cgaaaacata gtcatgaagc agagggcgaa
     2701 ttgattgaat cattgaaaaa tgaaatttga tcttgatgaa tagataaaaa aaaccgttca
     2761 gatctgaaaa taatctgagc ggttttttga gtaaaaatag tttatgatta ttcttaatta
     2821 aacatctatt tatttttaaa gctttcattt atcgataaga atagtttaac tgttgattta
     2881 ttccattttc cgttcatctg gtacgatgcc ttgacttagc aaccacttgt ggaagaacca
     2941 ttctgccaca ccgatcaata gagcaggaat caatactttc atccaaatat cgactgtatc
     3001 attcataaaa tatagataga gccacaagat aactgcagct gaaccagcat ctgcgatcgt
     3061 cgctgcgatg ttccctactt tacgtaagat caacagatcc cctagcacat agatcatgac
     3121 tacaataatt accccaacaa tcgttgaatc cataaagcta acaccgtata aacctgtcag
     3181 cacgacccac agaatcgccc agatcatgat tgccttcact accaacgcct tcacatgttt
     3241 cattttctgc actcctttct cctggataaa aaagtaagat tatctaacaa gataaccttt
     3301 tcattattat accatcggct gagagaagcg agcaaagata aggtcctatc taaaaatgag
     3361 gaagccactt agaacattca cgtaataatt tatccgtaca gacattcgat atggtgttcc
     3421 tttactttcg catactgttc ttttgtcagt tctttacttg tcaccagata atcgatttca
     3481 tgaaaagcaa tggcttgatg catgaattta tgtgcaattt tttgctgtgt aactaaaacg
     3541 gcaagccggt ctgattgttt tctaaaagct tttttgacca ttgcctctcc ttcatgagtt
     3601 tctgttactc cttggctgaa atctagcccg cgacaactca ttatcgctaa atccgcataa
     3661 tatgacaatg catcgttata ggcttttgaa ccgttgattg ttgctcgttt cggaacaatt
     3721 gcccctccca atacagatac ttttgccgtt gtatattcac tcaacatagc agctgtgacg
     3781 atgccattcg taatgatctg caaaccatca aatcttgcta ggtttctgac taagtgtaaa
     3841 gaggttgtac tggaatccaa gaataagacc atatcatctc gaatcaaaga agaagctgta
     3901 tctgcaatct tacgtttttc tgtttcttct tctaatttgg tgatcttcat caggtaaggc
     3961 acgtcaattt ttcgattgtc aataagcatg gcgccaccgc gaactctacg gatcaatcca
     4021 cttttttcca gaaaaacaag gtcacgttta atcgtggctt cgctataatt tactaactcg
     4081 atcaaatcgg tgactcttgc aaattggttg ttttttagat aattcacaat ctcctgctgt
     4141 ctattctcca cttcattcaa cataaaatca ccccatttac tttatatagt aacaggaaaa
     4201 aagaggttgt gacagaagtg attggcttcg agaaataaga agaaattttt cgaaaaaact
     4261 acttctgaac atcgtgtgtg gttaagaggg tatgacaaga ctattgtcat accctcttga
     4321 ccacacattt ttatttttag tataaactag ccttgcctac tgtgttcaaa atttccattt
     4381 tatgtttgac cacttcttgt acctttttat tagctgctgg ataaattgca ttcggctcgt
     4441 acatcgatgg attttcactc aatatttctc tcaaagcatt gaaaaagacg cttttaagat
     4501 ctgagctcaa attcactttg gcaactccgt attttactgc ttcgctgact tctgcgtccg
     4561 gattacctga accgccatgt aaaacaaatg gaagatcgat ttgttggttc aattctttca
     4621 gtaagtcgat atttaatttt ggagttctgt ttttcggata caagccatgc gctgttccaa
     4681 ttgctacagc aagtgtatcg attcctgttt ctttcacaaa aatcttcgct tgatcaggat
     4741 ctgtataaat gattgtttca gcacctcctt cagcagaacc attattccca atcgttccaa
     4801 gctcagcctc cactgagaca tttaccgcat gagctaacgc aaccactttt ttcgtaatct
     4861 gtacattgtc gtcgaatgat ttcaaagacg catcgatcat cacggaagaa tagccgtttc
     4921 gaatcgcccg taagacatct tctatcgtac cgccatgatc caagtggata acgatcggca
     4981 gtttacttgt ataagcatag gctcttactg ccgcaacaaa ctcatcactt aaataggcga
     5041 tttcatcagg atgaatttct aagattactg gtgcttctaa tctctctgct tcttcgatga
     5101 ttgctttcat catatcatag ctgcaaatat tgaaggctgg tactgcaaat tgattctctt
     5161 tagccacttt caataactct ttcattgtat ataacattac gcttccacct catttgttag
     5221 attttcattt ttcgtccact tgtcttgttt tttctctttc actgtttttt tcaatagtcc
     5281 aagcataaag gctgtagtga ctgtacctga tgcaattgcc aagacataca taggtaaatt
     5341 gttcataaca tttggaatta gcatcaccca aatccctcca tgaggtgcca ttgaagtaac
     5401 tcctactgcc atggaaatcc ctgcagcgac tgctgaacct gtcatcaaac taggtatcac
     5461 gcggatcggg tcagataccg caaacggtat agcaccttct gtaatatacg aagcgcctaa
     5521 aatccagcat gattgacctg aagtccgttc ttctttggta aatttatgtt tgaataataa
     5581 agtggctaaa gcaataccta ggggaggtgt cattcctgca gccatacaag cagcaatcgg
     5641 tgcatttact ccagctgaca tcaaaccgat cgagaaagtc gaagtcgatt tattgatcgg
     5701 cccacccata tcagctgcca tcatgatccc gataagtaat ccgaacaata gcccggatgt
     5761 atttcccaag ctattcagcc aatttgtcaa agcatccaac acccatgtga tcggttgttc
     5821 aatcacaaat accatcaaaa gcccaataat taagatcgat aaaaagggga cgacaatcag
     5881 ctgataaata gcagaaaatt ctttttttac gctaatttta tttgccagaa aatcacagat
     5941 ataccctgct agcacgccac caatcattcc gccaagaaaa cctgaaccac ctgtagaggc
     6001 cagtaatccg gcaacaattc ctggggcaaa acctgctttt ccagcaatcg atgtagcaat
     6061 ccccgcgcct aacgcaggaa ccatcatggc aaacgctgta tctcctccga tccttgaaaa
     6121 tgcagctgct aacacattat agtctgctga gttgggatca gccgctgtaa tcccaaaggc
     6181 aaaactcagt gcgatgatga ttccgcctgc gatcacaaat ggaagcatgt aattcacacc
     6241 attcatgaaa tgattataga ttgtcggctt tttctcattt gcagctggtt ctttttcatt
     6301 agacacaacg aatacgccaa tatctctcaa tgcttcgtct attactactt gcggttcttt
     6361 gattgctcgg cttgtagaga cacgctttac ttttttcccg gcaaatcggt ccaattcgat
     6421 ttctttatct actgcaagaa tgattgcatc tgcctctttg atttcttgtt cagataactg
     6481 attttctgtt tttacacctt gtgtttcgaa ttttgcttga tggtttagat ttcttgctgt
     6541 tacttctaat ttttctgccg acatataagt atgagcaatt ccagtcgggc aagcagttac
     6601 accaacgatt ttcatgatta ttttcctcct ttttcttcac tatcgatact cggacaaaga
     6661 ctctactatt ttcattcttg actaatctgt ctgatgatat ctgctggcgt agtggcttct
     6721 tttattccct ttctaaaatc tgcattcatc aatcgtctgg agagtgtgct gagaatctct
     6781 agatgcatat ctgctgattt ttcaggaact acgatggcaa aaatatattc aattggcatt
     6841 tcatctatcc cgctatagtc cgttaatttg gctgtatgaa aaaaacacag tgctggtttt
     6901 gtcacatatt tgctttttgc atggggaatc ccaaatccat cttctattcc tgtggagatc
     6961 tcctcctctc gtttgaatag tgccttaacg actttttgaa catttcttac cttatttgtt
     7021 tttttcaaca catcccctat tcgctcaaat aatgatcctt ttgaatctat cgatggatcg
     7081 aaaacaatca gttcttctgc gatcaattga tcaaacctca taaaaaacct ctcccccaca
     7141 aaaaatgtta acgttttctt ggaactgagg tcagtatagc atcgctagct gtaccgttca
     7201 atattatgaa tgaacagagg aaaaagaccc atcatccgcg tgtataagtc attaaaatga
     7261 acgatctagc tcacaaacaa tcgcaagtct ataacaaaac aaaaagcctt ccttgaaagg
     7321 aaagcttagc ttctaatttt aggtaaatgg ttttttactt cgaaataacc cctcttcatt
     7381 tcgttaatat aaggtgtttt tatcaagtgg ataattaaaa ctatatacta gtttttcatt
     7441 gttttgtgca ttatgatcct acgacttttt cagcatcaaa cgaagtttta aattggcaaa
     7501 aaacataaaa aaacagacta tccctatcag atagtctgct aaacgttgag ttatcagcgt
     7561 ttttattatt ccgcagcgtt tgcgtctttg agaccgtatt ttttgttgaa acggtccaca
     7621 cgtccgtctg cttgtgtgaa tttttgacgt ccagtgtaga atggatgtga gtcagaagtt
     7681 acttcgacac ggatgactgg gtatgtatta ccgtcttccc attcaactgt ttcttgtgaa
     7741 gtttttgttg aacctgacaa gaatttgaaa ccagttgttg aatccatgaa tactactggg
     7801 tggtaatctg gatggatgtt ttctttcatg attttttcgc tcctttgccc tgctccattt
     7861 gctgaaccag agttgatttg tcgatttaca acattaacat cttaacatgt ttcagtcaag
     7921 gttgcaatta tcttttcaga tttctggtcg gatttttttt accaaaggag acatcttgaa
     7981 attctttgaa aaattgctga ttgttttttg ttttacgcaa aaagcggatc agttgttccg
     8041 tatattctaa tgaatctcca gtcatatttt ttcgaagttt ccaaatttct tctaattctt
     8101 cacttggcat caataattct tcttttcggg tacctgattt tttgatatcg atggctggga
     8161 agatccgacg ttcagataat tcacgtgaaa gttgtaattc tagatttcca gttcctttga
     8221 attcttcata gatcacgtca tccatacggc ttcctgtatc gactaaagcg gtcgctaaaa
     8281 tcgtcaaact gccaccttct tcgatgtttc ttgctgcccc gaaaaatttc ttaggtttat
     8341 acaatgcggc tgggtcaatc ccgccactca atgtacgtcc gcttggtgga acgactaagt
     8401 tataggcccg agcaagtcta gtaatactat ccatcaagat aacgacatct cgtttatctt
     8461 cgactaaacg cattgcgcgt tccaatacta attcagaaac tcgagtatgg ttttgcggct
     8521 gcagatcaaa tgtagaagat accacgtcgc ctttcacgct tcgttctaga tccgttactt
     8581 cttccggtcg ttcatcaatc agtagaacga ttaattctac ttccggatag ttttcggtga
     8641 taccatttgc gatttctttt aatacagaag ttttccctgc ttttggcggt gcgacgatca
     8701 atccacgctg accaaaaccg attggagaaa agatatcgat cattcgggta gatagtcttt
     8761 gcggtgttgt ttccagttga agctgtcttt gcggataaag aggtgttaag gctggaaaat
     8821 gcggacgttc ttttgcttct tccggatctt ttccattaac gctttccacg tgcatcaaac
     8881 cataataacg ttccgattct ttaggtggac gggcttttcc tgccactttg tccccatttc
     8941 tcaaactgaa tcgtctgatt tgtgaggcag aaatatagat atcttctgca cttggtccat
     9001 aattgattgg ccgtaaaaaa ccgtaaccat cttgaccaac gatatccaat attccttcca
     9061 tataataaaa tccttgtttc tctgcttgtg cacgaatcac agctagagat aattcctttt
     9121 tattcatctt actgtaataa ggaattttaa attctttggc ataactataa acttctttta
     9181 acgttttgtt ctctagttct gccattgtca aataatcgct cattcagctc ccacttcctc
     9241 tatcatttca attgatgccc ctaaggctgt tagtttctcg ataatatgat cataaccgcg
     9301 taagatataa tctacattgt aaatcgtcgt tgtcccttct gccaatagtc cagcaatcac
     9361 taaacaagca cctgcccgaa gatcagaagc cacgacttct gtgccgtgaa gtttatttgg
     9421 accattcacg atgatcatat ttccttccac ggtaatatca gcgcccattc gtgctagttc
     9481 cggcacatgg ttcacacgtt tcgcatagat cgtatcgatg atttctgaag tgcctgtagt
     9541 catcaatagt aacgcagtaa gtggctgctg cagatctgtg gcaaatcctg gataaggata
     9601 ggtcatgatg tttgcgggtt ttaattcttt cgaggggtaa acttcgattt catcttcgct
     9661 gattttcata tggacaccaa tttcctgcaa ttttgctata aagctttcca aatgttcaaa
     9721 gatcacattt ttcactttga taccatttcc tactgcagct gctaatgcta gataggttcc
     9781 tgcttcgatg cggtcaggaa tcatgaaatg ccgacagcca tgtaatgttt cgactccttc
     9841 gatacggatc acatctgtac ccgctcctcg aacttttgcc cccatattat tcagtaaagt
     9901 tgccacatct atgatttccg gttctcgtgc agcgttttca attactgtct gtccttttgc
     9961 ttttacagca gctaacatca cattgatcgt tgctccgact gaaaccacgt ccatgaaaat
    10021 ccgattgcca cgcaatcctt tattttctgt ccgtaaatac atggcaccat gttcatttgt
    10081 cacttcagca cctagagctt caaatccttt gacgtgtagg tcaatcggtc tagggcctaa
    10141 ataacagcca cctggcaggc ctactaccgc ttcgccaaat ttaccaagca gtgttcccat
    10201 aaaataataa gaagcacgca aactattgat ttttccgtgc ggcatcggaa tcgatacgac
    10261 ttctcgagga tcgatttcta gtatgttgtt ggaaaaatgg acagttgcgc ccatgatctc
    10321 taaaatttca attaatgaat gaacatcttg tatgtccggc acaccttcca agatcactgg
    10381 ggaatcagct aagattgcag ctggaatcaa tgcgactgca ctattttttg caccgctgat
    10441 cgttacttca ccagctaaag gtcggtttcc tttgattacg atttttttca tctgtttcca
    10501 ccttgtttat taatcttttt tcatggatat gcttgtattt tatcatagat aaaaaaaaga
    10561 aacgagcatg gtctgccgtt ttaagtaaaa aagaatggag agcgacttca aaagtcgttc
    10621 tccattccta tatatcgtat ctgttatgtg gatgcttacg ctttgttagc tgaaccgaac
    10681 cagtcgatat gagatttagc atcttcaacg attgctgctt tacctggagc taataattta
    10741 cgagggtcga atcctttacc ttctttatct ttaccagctt cgatatattt acgagtagct
    10801 gcagcaaatg ataattggaa ttcagtgtta acatttactt ttgagatacc cattgagata
    10861 gctttttcga tttgatcttg aggaatacct gatccgccat gtaatactaa aggcatgtct
    10921 ggacctacag catcagcaat tgcttgtaag tggtcaaaag ctaatccttt ccagttttct
    10981 ggatattgac catggatgtt accgataccg caagctaagt agtcgatacc tgtagcaacc
    11041 atttgtttgc attcttcaat atcagctaat tcgccgttac cgatgattcc gtcttcttct
    11101 ccgccgattg aaccaacttc acattcaact gagatacctt ttgcatgtgc tttttcaaca
    11161 acgtctttag ctaatttcaa gttttcttca aatggtagat gtgaaccatc gaacattact
    11221 gaagtatagc ctaattcgat acattccaaa gcagcttcgt aatcaccatg gtctaagtgg
    11281 atagcaactg gaacagtgat gttcattgct tcaactaaat cagtgattaa atctttacag
    11341 attttgtaac cacccatgta tttagctgca cccattgatg tttggataag tactggtgct
    11401 tgttttgctt cagccgcttc aaggattgct tgagtccatt ccaagttgtt tgtgttgaat
    11461 ccgccgacag cgtaaccgcc tttacgagca gcttttaaaa attcagctcc tgatactact
    11521 ggcataataa aattcctcct aagttttgta aaactaaatt tgttaagaca attcttaacc
    11581 aacgacccta ttttaccaaa catttcggta gttttctact aaaatcaatg aaaatgaaaa
    11641 ttttcaagaa gttttttgca aaaaataaaa tatccaatag cgctttaatc gaaaaaagtc
    11701 ttttgctttc tatctttttt cctacaaata ataacaaatc agaaaaagag aaaagtgaca
    11761 agaaaattgc ttatcccctt ctcttgctac ttttctcttt ctatcaagtt caaaaattat
    11821 tttgtttcgt gatctgctaa tgatgctccg acaaatgctt tgattaattt ttgcgggcga
    11881 tttggacgag aaatcaattc tgggtggaat tggcatccca caaagatttt tttctctggc
    11941 agctctacga tttctactaa acggttatct ggtgagacac ctgaaaagac tagcccttgt
    12001 tcttcaaaca attgacgata tttgttattg aactcatagc ggtgacggtg acgttcttgc
    12061 acaacatcgg ctccatcata tgcttcagcc gtcttcgttc cttttttgat tttacatggg
    12121 tataatccaa ggcgtaaagt tccacccaaa ttctcgatgt tttcctgatc agccattaaa
    12181 tcaataatat tgtttgctgt atctggattc gtttcagctg atgacgcatc ttctaatcca
    12241 acgacgttac gtgcaaattc gacacaagcc atctgcatac caagacaaat acctaaaaac
    12301 ggaacgtcat tttcgcgagc ataacgaatc gcttcgattt tcccttctac tccgcgatca
    12361 ccaaatccac caggaaccaa gattccatct gcgtctttca aacgttcttc tacattttct
    12421 ctcgttaatt cttgtgcttt gatccaatcg atctcgatgt ctgaattata ggcgaaacca
    12481 gaatgtttca atgcttcaac tactgagata tatgcatctg gtaactcgac atatttaccg
    12541 acaagcgcaa tccgtacttt tttcttcaag ttcaagacac gttcttctaa ttctttccat
    12601 tctgtcatgt ctgcttctgg tgtatctagt tttaaatggt cgcaaacaat ttgatccatt
    12661 ccttgtgctt gtaaaactaa tgggatggag taaagtgtat ctacatctgg cgactcgata
    12721 actgcttctg gttcgacatc acaaaattgt gccaatttgt ttttcacgct ttgagaaaca
    12781 ggttgctctg tacgtaccac caaaatattc ggttggatac ctaaaccacg tagctctttt
    12841 acactgtgct gcgttggttt agttttcatt tctccagcag ctttcaagta aggaatcaat
    12901 gttgtatgga tatacataac attatctgta ccgacatctg ctttcatttg acgaagtgct
    12961 tcaaggaatg gcaaagattc tatatcccct actgttcccc ctacttctgt aatgatcaca
    13021 tctgaatcag tcattttagc tgctcgcatg attttttctt tgatctcatt tgtaatatga
    13081 ggaataactt gaacagttgc tcccaagtat tcgcccttac gttctttacg caaaacttca
    13141 gaatagattt ttcctgtcgt cacgttggag tatttgttca aattgatgtc aataaaacgt
    13201 tcgtagtggc ccagatccaa atctgtttca gcaccatcat ctgttacgaa tacttctccg
    13261 tgctgatatg ggctcatcgt ccccggatcc acgttgatgt atggatcaaa tttttggata
    13321 gttactttta agccgcggtt tttcaataag cgtcctaaag aagctgcaac aatccctttt
    13381 ccgattgatg aaacgacacc gccggtcaca aaaatatatt ttgtcataag ttcaaaaact
    13441 cctttttaat ttatgtgcag gaaaagtctc agacaataaa caaagctccc tattcttcaa
    13501 taagaatagg gagccgaaat ttctgtaaac ttctgaccct ctaaaagggt gcccaaggtg
    13561 tataatacaa gctgactaag ggaaagtcaa gtactcaaat gaatactatc cgcttacaat
    13621 tttttctttt tcgatatcct ttgagaaatt atttcttaag tcctttatga gtgcaaaaaa
    13681 aatttaaccc acataataag cttgatataa acatagctaa cgttaaacct aagcaaatca
    13741 gaaaatcgtt actgaaaaat tttaagtgaa ataatagata aaaaaataaa acaaacatta
    13801 tgatagataa tatcccacct aacaaagaac tactttttcc tacttgcagc aacagtttat
    13861 atagcttatc gtagaaacaa tctttttctt tcacccttat ttacctcctt tacgtaaaaa
    13921 acgtagtcca cagttctcgg atttttggca tatcttttct attaaataat atcaaaaaca
    13981 aaacaattcc actaattttt tcaaagatga cctttttttc aacaaaagtg ccttgtaggg
    14041 attcattgaa ggtaacgaat cacataagta aaaaataagg agatgcgaca aaagtcgtgc
    14101 ctccttattt ctaaataaac ggtgttctcc tgccaactcc tcggtcacaa gtcacaatat
    14161 ccatcaaaca tgagaagctg tttttgaaat tatttcttgt gattcggagt taaatggtag
    14221 gctcacaacc tctggataaa cggtattcaa aagccagttt ctcggtatta agccaaattt
    14281 atgcaaaaat gtagaaagcc attttcacaa aatttttctt aatactcgaa actaaacgtc
    14341 tttttcaaaa cctcttggta aacggtgttc aaaagcttgc atctgcggta ataagttcag
    14401 ccaattcaaa aacaagaaaa gctgtttttg cttggctatt cttattactt gttgcagggc
    14461 agctttttca caacctctcc actaaacggt gttcaatcag ctgaccctcg gtattagctc
    14521 aaaaaacggc aaaatatgag gaactatttt tccgttagtt tctctaatac tcaggtcaaa
    14581 acgctgattt cacagcctct aattcacggt attcctgttt taataaagtt ctagatattg
    14641 ttctctttcc cactcggaaa cagtttgtct gaatgccgcc cattcgagac gttttgcttc
    14701 aacaaaattt gagaagatat gttgtcctaa tgcgtcaatc attacattat ccttacgcaa
    14761 ttctttgata gcattatgga tagttgaagg taagtcctgg atatgtgcag cttctctttc
    14821 ttcctcatcc attacataaa tgttacggtc aacagctgct ggcggtgtga tctgattacg
    14881 gataccgtct agtcctgctt gcaacaatac tgccattgcc aagtatgggt ttgcagatgg
    14941 atcaactgac cgcaattcca aacgtgtgga taaaccgcga gattctggta cacgtactaa
    15001 tggcgaacga ttacggccac tccatgcgac ataaacaggc gcttcataac ctggaacgag
    15061 acgtttataa gaattgactg ttggattgca aacggctgtg taggcacgag catgtttcag
    15121 caaaccacct aagaaatgat aagctgtttc acttagttcc aattcaccgt ctttatcata
    15181 gaaaacattt ccttcatcat tgaacaaaga catattgcaa tgcataccag aaccattgat
    15241 cccaaataac ggtttcggca tgaaagtcgc gtgaagtcca tgcttgcgtg caatcgtttt
    15301 gacaacgagc ttgaaggttt ggatattatc acaagcatct accacatctg catatttaaa
    15361 gtcaatttca tgctgtcctg gcgctacttc atgatgtgaa gcttccactt caaagcctag
    15421 actttctaat tccaacacga tgtcacggcg gcaattttca cctaaatctg ttggtgcaaa
    15481 atcaaaataa ccacctttat cattcaaggt cgttgtgatc ccaccgtctt cgtctaattt
    15541 aaataagaag aattctggtt caggtcctaa attgaaagag gtaaaaccaa ggtcgcgcat
    15601 atctttcaat gctcgtttca aattcccgcg aggatcgccg gcaaatggtg tcccatccgg
    15661 attatagata tcacaaatta aacgagcaac ttttccatga gcactttccc acgggaagat
    15721 catccaagtg gagagatctg gatacaaata catatcgctt tcttcaattc gaacaaatcc
    15781 ttcaatcgaa gaaccgtcaa acatcatttt attttctaat actttgtcga tctggctcac
    15841 tggaacttct acatttttga tcgtacctaa gatgtctgtg aacatcaatc gtaaaaatcg
    15901 tacgttttct tcctcaatga ttcgtttgat ttcttcacct gttatctgtt tctttaccat
    15961 ggtatccctc ttcctttttt aatttattca gtcaaagaat tgtctttgca atccttgcga
    16021 ctgataatga cctgttttgc ttaacccacc ttgcgccaaa atctcatcac gaaaaatttg
    16081 tcgaacgtca tgatctgtca acggcttctt gttttttgct tcttgttttt tcgcctgtgc
    16141 catttcgtat acacgtttga tcccagccat attcaatccc tctgaaagat agtctttaat
    16201 ctcaagcaac atatcaatat cattcagcga atacatacgt cggtttcctt cgcttcgttc
    16261 cggatgaact aaatcttgtt cttcataata gcgaatttgc cgagcagaca aatcagtcag
    16321 cttcataact gtaccaattg gaaatacgga catagatcgg cgcaattcct tttctcccat
    16381 cagacgtccc ctttcgtttt ataagttggc taaagtgtac caattaaaaa aaatattgtc
    16441 aactgcatat gttaggtttt ctaacatgag atctctattt tttataaaat aacaaaaaaa
    16501 taagaacaca aaaaaggtat ctagttgatt ttcgtaccag atacctttta ttttttatta
    16561 tactgtacta tcctttatta gtgtttttat t
//
LOCUS       DAZKLD010000057        16588 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.057, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000057 DAZKLD010000000
VERSION     DAZKLD010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16588)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16588)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16588
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.057"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(64..1173)
                     /gene="rpoD"
                     /locus_tag="ACG8KR_001506"
     CDS             complement(64..1173)
                     /gene="rpoD"
                     /locus_tag="ACG8KR_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HFQ0200875.1"
                     /translation="MANGTDKKTYEAAVAAFIKENKPKRQVVYDDLSNQLATPFTLNA
                     DEMDKLIQKIEDAGISVVDENGDPSIHSLKSAEKKAEQAKTEDLSAPTGVKINDPVRM
                     YLKEIGRVSLLTAEEEVALALKIEEGDQEAKQRLAEANLRLVVSIAKRYVGRGMQFLD
                     LIQEGNMGLMKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETIN
                     KLIRIQRQLLQDLGREPTPEEIGAEMDLPTEKVREILKIAQEPVSLETPIGEEDDSHL
                     GDFIEDQEATSPAEHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVG
                     KVFGVTRERIRQIEAKALRKLRHPSRSKQLKDFLE"
     gene            complement(1197..3038)
                     /gene="dnaG"
                     /locus_tag="ACG8KR_001507"
     CDS             complement(1197..3038)
                     /gene="dnaG"
                     /locus_tag="ACG8KR_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HFQ0200876.1"
                     /translation="MAQRIPQEVIETVRSQTNIVEVIGQYVQLKKSGKNYLGLCPFHE
                     ERTPSFSVAEDKQIFHCFGCGKGGNVFTFLQELEGLSFPEAVVKVADFEQIPLEDQWR
                     QRTIAVQNPESATGKLIAAHEKAAEIYHHMLLHTKAGQPALEYLQSRGLSLELIEEFN
                     IGFAPNERSFLQQVFKNESISNELLERSGLFVTHDDGRMSDRFYQRVMFPLRNSQGQT
                     IGFSGRWLAPEKGQEKDQPKYLNSPETELFNKRQVLFNLDKARSDIRKNGEILLFEGF
                     MDVIAAWQAGIKTGLASMGTSLTAQQIQQMEKLTKELVFCYDGDNAGFEATNRGIELL
                     RQNSRLALSVVVVPEKLDPDEYLRKYGEASFQKLVAHGRETVFTFKSRYLKQGKNLDN
                     EKDKIEYLEELLVELSRVVSPIEQDMYLSQLSTEFQIGRETIQKQLRDLRRTNQQNRQ
                     EPQESQHTQVTRQETARKKRPLTQVEKAEQILLYRAFKEVSVRNILKEREFQFIHDAY
                     AEVYFLFDAYVSQHYEFNLAEFLDFLQDENLRRLVVEIEYLPVAEESSPREIQDLLDV
                     ISKSGLADEITLKKQMQQEARQTGNKQLELELTIEIINLTKQLKQAK"
     gene            complement(3220..5535)
                     /locus_tag="ACG8KR_001508"
     CDS             complement(3220..5535)
                     /locus_tag="ACG8KR_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFQ0200877.1"
                     /translation="MNGLTQQEVVQKMASGQQNDYQEDASKSTKQIFSDNILTLFNFL
                     NFGIGICLLFVGAYSNMAYLAIIVVNIVIGIYQEIHARNLVRKLSIVSQSEVLVIRDG
                     KEQSISTKELVLGDLVKIGAGEQIPADLQVISGRAEANEALLTGESDLIVKETGAELL
                     SGSFLTSGSVMAEVIRVGADNYAVRLTSEAKTHKAIRSELVDSIRKVSKFTSFVIIPL
                     GIILFLEALFIRDASIKISVIASAAALLGMLPKGLVLLISIALTTGVTKLAKKRILVQ
                     DMYSIETLAHVDTLCLDKTGTITEGKMSIQRVEALHPLYEKAIPEIMGTYLAESSDNN
                     ITMRAMRDYFELSNRYDVTNKVAFSSDRKWGAMEFPELGVVYVGAPERLVEEDRLPDS
                     LKTAQANGYRVLMLGIAPNQQLNEEKPAFVEPVALFEIDDPIRKNAQETLAYLHEEGV
                     DLKVISGDNPFTVSNIARRAGLPNYDAYVDLSQITEEMEVREAAHRYTVFGRVSPQQK
                     KLLVNELKESGRTVAMTGDGVNDVLALREADCSIAMAEGDGATRQIANLVLLDSDFTT
                     LPEVLFEGRRVVNNVTKVSGIFFIKTIYSFILSIICAVTAIGFPFIPIQITLIDLAIE
                     GYPSFFLSFEQDKRKITYRYLPTALINALPNALLVVVNIIAVYLLGKTFAWGNAETTT
                     LMYYLLIGISCMAVVKACYPFNPLRIFLAVTTIVGIYVAAMLFHGILEVNLGLGESVG
                     YFFVFMLVNIVLRVLYWKADIQNQLLKRVAN"
     gene            complement(5720..6580)
                     /locus_tag="ACG8KR_001509"
     CDS             complement(5720..6580)
                     /locus_tag="ACG8KR_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HFQ0200878.1"
                     /translation="MEKRQKMENQEVGYTTKDWMLRFVKGMFIGSGFILPGVSGGALA
                     AVFGMYERLISFLAHLTKNFKENVLFFLPVGLGGVTGIFLLSFLVSFLLGAYETTILW
                     FFVGCIAGTVPALWKEAGKKGRDTSDVIIMVVTFVLAYLFLLYGAQLFDGQVDQNFGT
                     WLIAGGLIGLGMIVPGLSPSNFLVYMGLYKAMADGFKEVRLEVIVPIGIGGILCVLGL
                     SKIMDYIFSKAYSKLFHFILGVVFASTIMIIPTDYAGLGVAGVLACPVLFIAGTALGW
                     WMSRLEEQYK"
     gene            complement(6639..7271)
                     /locus_tag="ACG8KR_001510"
                     /pseudo
     CDS             complement(6639..7271)
                     /locus_tag="ACG8KR_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289460.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme family protein"
     gene            7438..8199
                     /locus_tag="ACG8KR_001511"
     CDS             7438..8199
                     /locus_tag="ACG8KR_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFQ0200879.1"
                     /translation="MTDIIVILYTVGDYVKKEKKYAKMPDGSEIYYEKSGQGFPLFLL
                     HGNDGSGRFFSEQVPVLERYYTVYLVDSRGHGRSTNEASMLNFQLMAEDLNTIMLLEK
                     IDQADFLGFSDGANLALVFASSFPKKVHRLILNSGNTLVKGVRFSARVISNIHYAWVW
                     LLSLFRPSLRKNLLVIKLLLHDIGLTENDLKKINSPTLIIVGKKDVIKLKHSLYIAKT
                     IPKASFVLVKEQGHELARKDPERFNREVLQFLSET"
     gene            8225..10816
                     /gene="mprF"
                     /locus_tag="ACG8KR_001512"
     CDS             8225..10816
                     /gene="mprF"
                     /locus_tag="ACG8KR_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HFQ0200880.1"
                     /translation="MLKNTIQWLKNHLGLFKTIFLISVIVIIVGELMSIGKTLSIQQL
                     GETFATIPVWKSGLMLLIGLISVLPMVGYDIILNRMLEQKQNPRYLFETSWLINTINN
                     IAGFGGFVSVGLRSELYGQKKESRNVIQALSKVFLFLMAGLSIYSLLSFLLVVFTPVA
                     PFLKQYWIWLIGGGLYFPVVFLFTTFKKKGFLGGTSLKTQGQLLLVSLLEWTGVLFSF
                     LSVGYLMEIRIDLWQTIPLFIAASVIGIVSMIPGEIGSFDVMMIIGLSAIGVPREIVV
                     VWILLYRLFYYIIPFLIGIVFFFKNIGSTFDQRYSGIPKQLATEIAHKIVVVLLYFSG
                     IMLVLSATIPQAFTEFRWLHSLNPLKFHFIIQFPSILLGFLLIIMGRGIAARVKRAYL
                     PTIFLIVLALLYVLLSDFSFTPVVFLSILLLIILASKNELFREQLIYSWEWRTIDGII
                     IGALSLLYIVIGVYNLPDFPHRRHHFISFFLFPSEKIWFSGLLAIIAVSFMIVLFVHF
                     LQGEKKQIGEAFNEEKALKILTTYGGNSDSQLIFLKDKRMFAYEKDGEPTVLLQFACF
                     NNKCIVMGDPSGKKEDFPEAIEAFIEETDRLCYLPVFYETSEEIVMILHEFGYDFIKM
                     GEEAYVDLNSFTTSGKKMKGTRAVLNRIEREGFTFDVLQPPFSTEQMSTFKNISDNWL
                     GSRKEKGFSLGFFSEDYLQRAPIAVVKNTQEEIVAFATIMPTYTNNKVGTIDLMRYDS
                     KKAPSGSMDFLFLHLFAYMKEEHIQWFNLGMAPLSNVGTSRKSFLQERIAYLVYEFGS
                     HFYSFHGLREYKSKYATNWVSRYTLYSRDSWIAYVMIALLIIDNAPVEQTQGRISGLK
                     KWIRRKY"
     gene            complement(11004..12191)
                     /gene="tuf"
                     /locus_tag="ACG8KR_001513"
     CDS             complement(11004..12191)
                     /gene="tuf"
                     /locus_tag="ACG8KR_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014335040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HFQ0200881.1"
                     /translation="MAKEHYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLGKKGLANP
                     QDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQMDGA
                     ILVVSATDGPMPQTREHILLSRQVGVKYLIVFLNKVDLVDDEELIDLVEMEVRELLSE
                     YGFPGDDTPVIKGSALKALQGDPDAEAAIMELMDTVDEYIPTPERDTDKPLLLPVEDV
                     FSITGRGTVASGRIDRGAVRVGDEVEIVGIKPETQKAVVTGVEMFRKTLDYGEAGDNV
                     GVLLRGIQRDDIERGQVLAKPGSITPHTKFKAEVYVLTKEEGGRHTPFFNNYRPQFYF
                     RTTDVTGTITLPEDTEMVMPGDNVTIDVDLIHPIAVENGTTFSIREGGRTVGSGIVTE
                     IEA"
     gene            12559..12915
                     /locus_tag="ACG8KR_001514"
     CDS             12559..12915
                     /locus_tag="ACG8KR_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFQ0200882.1"
                     /translation="MKKLIGVLVVLGIFLGGSFAAYHYFYGGEAYYTKITTNGEMSAT
                     QADNGEKFTTYTYKQAAYNKDGEKKQVTLREEREHPLKMNAYLKLKVNPRKGVLSWEE
                     IKASEVPKNAAEKIDN"
     gene            complement(13156..14160)
                     /locus_tag="ACG8KR_001515"
     CDS             complement(13156..14160)
                     /locus_tag="ACG8KR_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HFQ0200883.1"
                     /translation="MDIYLKKAILHIIDRETGSPVFSQKELDLTKEYIRDFLQKKIQK
                     ISSAQTKTGQLAEESMIVESLKIAQENFVEASEAIVQRWFDIYQESEEAPSADVFVVL
                     YELDTVMYLSLLKVNYREAYTHFVEADEAGIDNRLILNRAILGAKTQKADEAFAVNLT
                     DWTYELIEKKYEFSGKKELYFSGRVIESVPAPSLEENVKVIQKVAKHLGKKFETEEFD
                     IMADLKEAVYDTIEEKGRLDHEMIAEKVFKENITAKMAFKEEVQEQGFVPEAPPVKEV
                     QEISEKKFGKQKFKLSNGIELIVPVDVYRNPDLIEFVNNPDGTISVMIKNVDEVLNRL
                     "
     gene            14326..15177
                     /locus_tag="ACG8KR_001516"
     CDS             14326..15177
                     /locus_tag="ACG8KR_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFQ0200884.1"
                     /translation="MNSLTSTYRLSNGYEIPVVGFGTWQTPDGDVAVSSVKEALAAGY
                     RHIDTAQGYKNEESVGQAIKESGIPREEIFLTTKLWNANHSYELVMSSFEESLKKLQT
                     DYLDLFLIHWPNPVAFRDNWEQANADTWRAFEELYEAGKIKAIGVSNFLPHHLDTLAK
                     TAKIMPMVNQVFLAPGELQTTVVEYAKKHDMILEAYSPLGTGKIFDVPEMKQIAEAHD
                     KTIAQVALRWSLQHEFLPLPKSVTPSRIKENTELFDFELTEEEMKQIDQLDGVVGKAK
                     DPDTTQF"
     gene            15194..15439
                     /locus_tag="ACG8KR_001517"
     CDS             15194..15439
                     /locus_tag="ACG8KR_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0200885.1"
                     /translation="MDPIERLNSLSEEVTQTFHSDFVFLIDAEKIQHFPARNWTHDQI
                     IEELKKRFDHSLMVKPWHEHEVIYSPELPVFALIPKK"
     gene            15626..16330
                     /locus_tag="ACG8KR_001518"
     CDS             15626..16330
                     /locus_tag="ACG8KR_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0200886.1"
                     /translation="MKKKQKQKLLNQFRPSLDGVRGQLFRSLEEESLRRYGLPLQVML
                     DPKNRQILTIGLAGKTNEDTRINVPIDDNFNTVLKRIRSGENGIFERFRDNLLIEIVS
                     YWNDQRLKNDEPTAQAVATPVTEETVSVPEEKTEKPKADTTENTRSTDTNAALSFADF
                     SKAVAEYPKFYTEQNEHEVVINEKNADEPRKLATISMTTENEFVIEKALERKYKVKLT
                     LIPLIEQFAATAITDR"
BASE COUNT         4890 a         3413 c         2927 g         5358 t
ORIGIN      
        1 tttttttctt ttaaaataaa ccaaacagtt ttactgattt ctcttctggt caatagtcca
       61 cgcttattct aagaaatctt ttaattgttt tgaacgagaa ggatggcgca attttcttaa
      121 tgctttagcc tcgatctgac gaatacgttc ccgagtaaca ccaaatactt tccctacttc
      181 ttccaatgtt ctcgtccggc catcatctaa accaaaacgt aagcgaagga cattctcttc
      241 ccgatcagtc aatgtatcta gtacatcttc caattgttct tttagtaact cataagctgc
      301 gtgttctgct ggacttgttg cttcctggtc ttctatgaaa tcacctaagt gagagtcatc
      361 ttcttcaccg attggcgttt caagggaaac aggttcttgc gcgattttca agatttctcg
      421 gactttttct gttggaagat ccatttccgc accgatttct tctggcgtag gttctcttcc
      481 aaggtcttgc agcaattgac gttggatacg tatcaattta ttaattgttt caaccatatg
      541 aactggaata cggatcgtac gtgcttggtc agcgattgct cgagtgatgg cttgacgaat
      601 ccaccatgta gcataggttg aaaacttaaa tcctttgcgg tagtcaaatt tttcaactgc
      661 cttcatcagt cccatgtttc cttcttggat caagtcaagg aactgcatgc ctcgtcctac
      721 ataacgttta gcgatactta ctactaaacg aaggtttgct tctgccagac gctgtttcgc
      781 ttcttggtcg ccttcttcga ttttcaatgc taacgctact tcttcttccg cagttaaaag
      841 ggagacacgg ccaatttctt tcagatacat acgaaccggg tcattgattt ttacaccggt
      901 tggcgcagaa agatcttctg tttttgcttg ttcagctttt ttctcagcac tcttcaagct
      961 gtgaattgat ggatcaccat tttcatccac cacactgata cctgcgtctt caatcttttg
     1021 gatcaatttg tccatttcat ctgcattcaa agtgaatggt gtagcaagct gattggataa
     1081 atcatcatat acaacttgtc gtttgggttt gttttctttg atgaaagctg ctactgctgc
     1141 ttcatacgtt tttttatctg ttccatttgc cataaaagaa ggcccccctt gagtagctat
     1201 ttggcttgtt ttaactgttt cgttaaattg atgatttcaa tcgtcaattc aagctcaagc
     1261 tgtttgtttc cagtttgtct ggcttcttgc tgcatttgtt tttttaatgt tatttcatca
     1321 gctaagccag atttgctgat cacatctagc aagtcttgga tttctcgtgg cgaactttct
     1381 tctgccaccg gtaaatattc gatttccacc accaaccgcc ttaaattttc atcttgcaaa
     1441 aagtctaaaa actctgcaag gttgaattca taatgttgtg agacatatgc atcgaataaa
     1501 aaataaactt ctgcatatgc atcatgaata aactgaaatt ctcgttcttt taaaatatta
     1561 cggacagaaa cttctttaaa tgcacgatat aatagaatct gctctgcctt ttctacttgc
     1621 gtcaaaggtc tctttttccg tgcagtttct tgtctcgtaa cctgagtgtg ttgactttcc
     1681 tgaggttctt gacgattttg ctgatttgtc cttcgaagat cgcgcagttg tttttgtatc
     1741 gtctctctgc cgatctgaaa ctctgttgac agttgagata gatacatatc ttgttcgatt
     1801 ggtgaaacaa ctcggctaag ttcaactagt aattcttcga ggtattcgat cttgtctttt
     1861 tcattatcta aatttttccc ttgttttaaa taacgtgatt taaaagtaaa gactgtttcg
     1921 cgcccatgag ccactaattt ttggaaagat gcttctccat acttacggag atattcatca
     1981 ggatcaagct tttctggaac gactacgaca gaaagtgcta atcgactgtt ttgccgaagc
     2041 aattcaatcc ctcgattggt tgcttcaaaa cctgcgttgt ctccgtcata gcaaaagacc
     2101 aattctttcg tcagcttttc catttgttgg atctgttgag ctgttagact tgtccccata
     2161 gaagctaatc cagttttgat accagcctgc catgcagcaa tcacatccat aaacccttcg
     2221 aacaacaaaa tttctccgtt tttccggata tcgcttcttg ctttatcaag attaaataaa
     2281 acttgtcttt tattgaacag ttctgtttct ggactattca agtatttggg ctgatctttt
     2341 tcctgccctt tttcaggtgc aagccagcgt cctgaaaaac cgatcgtctg gccttgagaa
     2401 ttacgaagtg gaaacatcac tctttgataa aaacgatcag acatacggcc atcatcatgc
     2461 gtgacaaaca atccagagcg ttctaacaat tcatttgaaa ttgattcatt cttgaatact
     2521 tgctgaagaa aagaacgctc attaggtgca aaacctatgt tgaattcttc aattaattct
     2581 aaagacaacc cacgactttg caaatattct aaagctggct gtccagcttt tgtatgcaat
     2641 agcatgtgat gatagatttc cgctgctttt tcatgagcag caatcagttt ccccgtagca
     2701 gattcaggat tttggacagc tatagtacgc tgacgccatt gatcttctaa cggtatctgt
     2761 tcgaagtctg ccacttttac cactgcttct ggaaaactta gaccttctag ttcttgaaga
     2821 aaagtaaaca cgtttccgcc tttaccacaa ccaaagcaat gaaaaatctg cttgtcttct
     2881 gcaacagaaa aagacggtgt acgttcttca tgaaaaggac acagccccaa gtaattttta
     2941 ccagactttt tcaactggac atattgtcca atcacctcaa cgatattcgt ttgactgcgt
     3001 actgtttcga taacctcttg aggaatccgt tgagccattt tttcaccccc tgctctattg
     3061 atatcttttg ggtttaacag aagatatttc aggagaataa ccggtaaaaa atcgcactaa
     3121 ctgaaatcag tgcgatttta aaggacacaa ttatacatgt ttgattgtat catatttcat
     3181 tgctttttca agtataacac atttctatct cattaagcat caatttgcta ctcttttcag
     3241 taactgattt tgaatatcag ccttccagta caatactcga agaacaatat ttacgagcat
     3301 gaatacaaag aagtatccta cgctctctcc caaccctaag tttacttcca aaattccatg
     3361 gaacagcatt gcagctacat atatccctac gatcgtcgtt actgctagga agatgcgcaa
     3421 tgggttgaag ggataacaag cttttacaac tgccatgcaa ctgatcccaa tcagcaagta
     3481 atacatcaaa gtagtagttt cagcatttcc ccatgcgaat gtttttccaa gtagatacac
     3541 cgcaatgata ttcactacta caagcaaggc atttggtaaa gcattaataa gtgctgtagg
     3601 caaataacgg taagtgattt ttcttttatc ttgttcaaaa gataaaaaga aggaaggata
     3661 cccttcaatc gctaaatcaa tcagcgtgat ttggataggg ataaacggaa atccaattgc
     3721 tgtaacggca catataatcg ataaaataaa tgaataaatc gttttgataa aaaagatgcc
     3781 tgagactttc gtcacattgt tgaccactcg gcgtccttca aacaggactt ctggcaatgt
     3841 agtaaaatca gagtccaaca ataccagatt cgcaatctga cgtgttgctc catcaccttc
     3901 tgccatagca atgctgcaat ctgcttctct taacgccaaa acatcattca caccgtcgcc
     3961 tgtcattgca accgttctgc cgctttcttt caattcattt accagcaatt tcttttgctg
     4021 cggactcact cgtccaaaaa ccgtatagcg atgagcagct tctcgcactt ccatttcttc
     4081 agtaatttga gaaagatcga catacgcatc ataattcggt aatcctgcac gtctagcgat
     4141 attggagaca gtaaaaggat tgtctccaga aatcactttc agatccacac cttcctcatg
     4201 aagataagct agtgtttctt gagcgttttt tcggattgga tcatcgattt caaacagagc
     4261 aaccggctca acaaatgctg gtttttcttc gttcaattgc tgatttggag cgatccccaa
     4321 cataagtacg cgatagccat tcgcttgggc tgtttttaga ctgtccggaa gccgatcttc
     4381 ttccaccaaa cgttcaggcg ctcctacgta cacgactccc agttctggaa attccatcgc
     4441 gccccatttg cgatcagaag aaaatgcgac tttgtttgtc acatcatacc gattagaaag
     4501 ctcaaaataa tctctcatag cccgcatagt gatgttgtta tcactgcttt ctgccagata
     4561 cgtccccata atttctggga tggctttctc gtacagcgga tgtagtgctt ctacccgctg
     4621 gatactcatt ttcccttcag taatggtccc tgttttatcc aagcataatg tatcgacatg
     4681 ggccagcgtt tcaattgaat acatgtcttg aacaaggatt cgtttttttg ccagtttggt
     4741 tacccctgtc gttaatgcaa tacttattag caataccaaa ccctttggaa gcatgcctaa
     4801 caaagctgct gcagatgcaa taacagaaat tttaatagaa gcatcgcgga tgaacagtgc
     4861 ttccaaaaat agtatgatcc ccaaaggaat aatcacgaaa ctagtgaatt tggatacttt
     4921 gcgaatcgaa tctacaagtt cagaacgaat agctttgtga gtctttgcct cgctagtcaa
     4981 gcggacagca taattatctg cacctactcg gataacttca gccatgacag aaccgcttgt
     5041 caaaaagctt ccagaaagca attcagctcc agtttctttg acaatcaaat cagattcgcc
     5101 tgtcaaaaga gcttcatttg cttctgcccg tccagatata acctgtaaat cggctggtat
     5161 ctgttctcct gctccgattt ttaccaaatc tcctaatacc agctctttag tagaaatcga
     5221 ttgttcttta ccgtcccgga tcactagtac ttctgattgg gaaacaatcg ataatttccg
     5281 tacaagattt cttgcatgta tttcttgata aattccaata acgatattga cgacaataat
     5341 tgctaaatac gccatgttgg aatacgcacc tacaaataac agacaaatcc caataccaaa
     5401 gttcagaaaa ttgaataacg tcaatatatt atcgctgaat atttgttttg tactttttga
     5461 tgcatcttcc tggtagtcat tttgctggcc agaagccatt ttctgaacta cctcttgctg
     5521 tgtaagtcca ttcattttct ttttctccat tttccgccaa ctttcaaaaa taatcacgtg
     5581 tttaagtcta gcgtgttcca agattaaaaa caacttgttt ttataaaatt tatagaaaaa
     5641 cacacgaatg attttacaaa aaagatgcca acctttttgt ttgaatcacc cgtgtgctgt
     5701 ttttattatc aatctatctt tacttatatt gttcctccaa acggctcatc caccaaccta
     5761 gtgctgtacc ggctataaaa agaacaggac aagctagtac cccagcaact cccaatcccg
     5821 cgtaatcggt aggaatgatc ataatcgttg aagcaaaaac tacacctaaa atgaaatgga
     5881 atagcttgct atatgccttt gagaaaatgt aatccatgat ttttgacaat ccaagaacac
     5941 aaagaatccc accaattccg atcggcacaa tcacttcaag tcgtacttct ttaaatccgt
     6001 cagccatcgc tttatacaat cccatataca ccaaaaaatt agaagggctc aatcctggga
     6061 cgatcatacc caaaccaatc aatccgccgg caatcagcca tgttccaaaa ttctgatcga
     6121 cttgcccgtc aaataattga gcgccgtata ataaaaacaa ataagccaga acaaaagtaa
     6181 caaccatgat gatcacatca gaagtatctc ttcctttttt ccctgcttct ttccataacg
     6241 caggaacagt tccagcgata catccgacaa aaaaccataa tatcgttgtt tcatacgcac
     6301 ctaataaaaa actgactaaa aaagaaagta gaaagatgcc tgtcactccg cctagaccaa
     6361 caggcagaaa aaacaaaaca ttttctttga aattttttgt aaggtgggct aaaaaagaaa
     6421 tcagccgctc atacatgcca aagacagctg ctaatgcacc accgctgact cctggcaaga
     6481 taaatcctga accgataaac attcccttca caaaccgtaa catccagtct ttggttgtgt
     6541 accccacttc ttgattttcc attttttgcc ttttttccac tatttgcgca ctccttcttt
     6601 catttaattt tattcatgat tgaacatttc ctgctatttt acttaaagac tttttcatag
     6661 gtctctatta ttttttcatt cattttgaca atatcaggat aaaacatgtt acctccatta
     6721 tgatagagat agccgccatt gtaaagcaag gactggatcc gccaatatcg gtattgtttc
     6781 ccttcctcat ttcctaaaag tgggctcagc acatctttag aatacgcttc tgctaattct
     6841 actgtgtttt ctccgccatg attttttaca taggcaatat aatctttccc aaaattatat
     6901 gcttggacag ctgtccataa atcgcatcct tgtttctttg cttctactat catttccgcc
     6961 aaatatttga caccttgatc aatactgtca gctggattat caatgatctc agcttctcca
     7021 tgcagacttt ccgagctcta catgagatct gtcccttttc cttttgattc ggtcaatatt
     7081 atcgccttga tcaaattctc gtagttttcc atatgataat tttttgcagc tgttgttatt
     7141 tctatttgat agctttccac tcgttttacc gaacgataag ttcttcctcc taaaaacaac
     7201 accgcaccaa taaccgtcag aatgatcatg taaaaaagaa tacggataat agataatcgt
     7261 tttcgcttca tttcatcacc tagttatttt gacatctttt tttatcttac tatttcttta
     7321 tcttcttggc tatttcttcc ttgatttctg tgtacgcctt aagaaaaaat gaattttcct
     7381 gtattttttg acatcttacg aaaatgatcg ttgtttttca gtaaaaacaa ttgcccaatg
     7441 actgatataa tcgttatact ttacacagta ggtgattatg tgaaaaaaga aaagaaatat
     7501 gcaaaaatgc cagatgggag cgaaatttac tatgaaaaga gcggtcaagg cttccctctc
     7561 tttttactgc atggcaatga cggcagcggc cgcttttttt cagaacaggt ccctgtactc
     7621 gaacgctact ataccgtgta tctagtagat agccgaggac atggtcgttc gacaaacgaa
     7681 gcatctatgt tgaattttca gttaatggcc gaggatctga atacgatcat gttgctggaa
     7741 aaaatcgatc aggcagattt tcttggattt agtgatggag caaatctagc actggtattt
     7801 gccagcagct tccctaaaaa agttcatcga ttgattttaa attctggaaa cacgcttgtt
     7861 aaaggcgttc gtttttctgc cagagtcatc agtaatattc attatgcatg ggtatggtta
     7921 ctctctttat ttcgcccaag tcttcgcaaa aatcttttag ttatcaaact tttgctgcat
     7981 gacatcgggc ttacagaaaa tgatttaaaa aaaatcaact cccccacttt gatcatcgtg
     8041 ggaaaaaaag atgtaatcaa gctaaaacat tctttgtata tagctaaaac tataccaaaa
     8101 gcttcctttg tgcttgtgaa agaacaagga catgaattag ctagaaaaga tcctgaacgc
     8161 ttcaatcgag aagttcttca atttctttct gaaacataac tattaggaat agaaggtgat
     8221 ttatttgtta aaaaatacca tacaatggct gaaaaaccac ctaggactgt tcaaaacaat
     8281 ctttttgatt tctgtcattg tcattattgt cggtgaattg atgtccatcg gtaaaactct
     8341 atctatccag caattaggag aaacgtttgc cactatacct gtatggaaaa gcggactaat
     8401 gctattgatt ggtcttatct ctgtgttacc tatggttggt tatgatatta ttttgaatcg
     8461 gatgcttgaa caaaagcaga atcctcgtta cctgtttgag acaagctggc tgatcaatac
     8521 gatcaacaat attgccggtt tcggcgggtt tgttagtgtg gggcttcgct ctgaactata
     8581 tggacaaaag aaagagagca gaaacgtgat ccaagcatta tcgaaagttt tcttgttttt
     8641 gatggctgga ctttctattt acagtctgct ttctttttta cttgtagtct tcacgccagt
     8701 tgctcctttt ttgaagcaat attggatatg gttgatcggc ggcggtctat attttcctgt
     8761 cgtcttttta tttactactt tcaaaaagaa aggattcctt ggtggtacct cacttaaaac
     8821 tcaaggacag ctattgctag tttcattgtt ggaatggacc ggtgtcctgt ttagtttcct
     8881 ttctgttggt tatttaatgg aaatccggat tgatctgtgg caaacaattc cattattcat
     8941 tgctgcttca gtgataggaa tcgtgtctat gattccagga gaaattggga gtttcgatgt
     9001 aatgatgatt atcgggttgt ccgctattgg tgtgccaaga gaaatagtcg ttgtgtggat
     9061 cttgctctat cgcctgttct actacatcat tccgtttttg attggaatcg tctttttctt
     9121 caaaaatatc ggttccactt ttgatcagcg ttattctggt atccctaaac aattagcaac
     9181 agaaattgcg cataaaatcg tggttgtctt attatacttt tctggaatca tgctagtact
     9241 ttctgccact atcccgcaag cttttacaga gtttcgctgg cttcatagtt tgaacccatt
     9301 gaaatttcat tttattatcc aatttcctag tatactattg ggatttcttt taatcattat
     9361 gggcagaggg atcgcagcac gtgtgaaacg agcatatttg cctacaatct tcttgattgt
     9421 tctagcttta ctttatgttt tattaagtga ttttagtttc acaccggtgg ttttcttgtc
     9481 tattctgctt ctgatcattc ttgcttctaa aaacgaatta tttcgggaac aattgatcta
     9541 ttcatgggag tggcgtacga tcgatggaat cattattggt gcactgtcct tgctttacat
     9601 cgttattggg gtatacaatc tgccagattt ccctcatcgg cgtcaccatt ttatttcctt
     9661 tttcttattt ccttctgaaa agatttggtt ttcgggattg ttggccatca tcgctgttag
     9721 tttcatgatc gttctatttg ttcatttcct tcaaggagaa aagaaacaga ttggcgaagc
     9781 ctttaatgaa gaaaaggccc tgaagatcct tacaacttat ggaggaaact ccgatagtca
     9841 attgatcttc ttaaaagata agcggatgtt tgcctatgaa aaagacggtg aaccaactgt
     9901 tcttctccaa ttcgcttgct tcaataataa atgtattgta atgggtgatc cttctggtaa
     9961 gaaagaagat tttccagaag caatcgaagc ttttatcgaa gaaacagatc gactatgcta
    10021 tttaccagtt ttctacgaaa caagcgaaga gattgttatg attttgcatg aattcggcta
    10081 cgatttcatt aagatggggg aagaagctta tgtagacctg aatagtttta caacttcagg
    10141 gaaaaaaatg aagggcactc gtgctgtctt gaatcggatc gaacgagaag gatttacatt
    10201 tgatgtctta caaccaccat tttctactga gcaaatgagt actttcaaaa atatctctga
    10261 taattggttg ggttcacgaa aggaaaaagg gttttcttta ggctttttct ctgaagatta
    10321 tctacaaaga gcgcctattg cagttgtcaa aaatacccaa gaagaaattg tcgcttttgc
    10381 aacgatcatg cctacttaca cgaacaataa agtaggaacg attgatctga tgcgatatga
    10441 ttctaaaaaa gcaccttctg gcagtatgga tttcttgttc ttgcatttgt ttgcctatat
    10501 gaaagaagaa catatccaat ggttcaattt agggatggcc cctctttcaa atgtcggaac
    10561 ttccagaaag agttttttac aggaaaggat tgcatacttg gtttacgagt ttggttctca
    10621 tttttattct ttccatggat taagagaata taaaagcaag tacgctacaa actgggtctc
    10681 acgttatact ctatattcta gagatagttg gattgcatat gttatgattg ctttgctgat
    10741 tattgataat gcgccagttg agcagacaca aggtagaata agtggtttga aaaaatggat
    10801 acgaagaaag tattaaaaac tgcataccga tagatgaaca ctctcatgat gaataaaaga
    10861 gactgaagta aaacgaaagt tcatgcttct acaatctctt cttatacaat tgaagaacaa
    10921 agagcggtcc aaacagaaat cctgtttgga ccgctctttt attttaaaca tttttcatta
    10981 ccaaaatatt cgagtcgcgt ttcttatgct tcgatctctg taacgatacc tgaacctact
    11041 gtccgtccgc cttcacgaat tgagaatgtc gttccgtttt caacagcgat tggatggatc
    11101 aagtcaacat caatcgtcac gttgtcacca ggcatgacca tttctgtatc ttctggcaat
    11161 gtgattgttc ctgtaacatc agttgtgcgg aagtagaact gtggacgata gttgttgaag
    11221 aatggagtat gacgtccacc ttcttctttt gtcaacacgt acacttctgc tttgaatttt
    11281 gtatgtggag taatggaacc tggtttagca agtacttgtc cacgttcgat atcgtcacgt
    11341 tggatccccc gtaacaacac gcctacgtta tccccagctt ccccgtaatc taacgtttta
    11401 cggaacattt ctacacctgt tacaactgct ttttgtgttt caggtttgat ccctacgatc
    11461 tctacctcat caccgacacg aacagcacca cgatcaatac gacctgaggc aacagttcct
    11521 cgacctgtaa ttgagaagac gtcttccact ggcaatagta atggtttatc tgtatcacgt
    11581 tctggtgttg ggatatattc atctactgta tccatcaatt ccataatagc agcttcagca
    11641 tctggatcgc cttgcaatgc ttttaatgcg gaacctttga tcacaggagt gtcatcgcct
    11701 gggaaaccat attcgctcaa taactcgcga acttccattt ctaccaaatc gatcaattct
    11761 tcatcatcga ctaaatcaac tttgttcaag aaaacaatca ggtattttac accaacttgg
    11821 cgtgacaata aaatgtgttc acgtgtttga ggcatcggac cgtcagttgc agaaacaact
    11881 aagattgcac catccatttg tgctgcccct gtaatcatgt ttttcacata gtccgcgtgt
    11941 cctggtgcat caatatgcgc gtagtggcgt ttttctgttt catattctac gtgtgcggta
    12001 ttgatcgtaa tcccacgttc acgttcttct ggagctgcat caatactagc atagtcttga
    12061 ggatttgcta ggcctttttt gcctaatact gtagtgatcg cagcagttaa agtagttttc
    12121 ccatggtcaa cgtgtccaat cgtaccaatg ttaacgtgtg gtttacttct gtcataatgt
    12181 tcttttgcca taatttacaa acctcctaaa taatatagat cactgaaaaa tttcagctct
    12241 caccttttta ttatagtctt tttttgacta aatcaaaaag aatacgtccg acttactttt
    12301 tgtaagattc gaactaagct tattatacgt caatattggt caaagtcaac taagagcact
    12361 cgtctgtctt atacgcgaaa aaagtacctt ttataaataa aaaaatgtgt tttttgagaa
    12421 aaacttcaag atagtcctta actttctttt gaagcaataa gaacaaaatg aggaaaagtt
    12481 taaaattcag ctgttgccaa caactttcaa tagacgaaat cttctatttc gttaataata
    12541 aggaaagagg tgaagtacat gaaaaaatta attggcgtac ttgtcgtttt aggcatattt
    12601 ctcggcggaa gttttgcagc ttatcattat ttttacggcg gcgaagctta ttatacaaaa
    12661 atcacgacga atggcgaaat gtcagctacc caagcagata acggagagaa attcacgact
    12721 tatacctaca agcaagcggc ttataataaa gatggtgaaa aaaaacaggt gacgcttcgc
    12781 gaagaaagag aacatccatt aaagatgaat gcgtatctta aactcaaagt caatcctcgt
    12841 aaaggtgtat taagctggga agaaataaag gcttctgaag tgcctaaaaa tgcggcagaa
    12901 aaaatcgata attagtaaat ggtcttaaaa ttttattaga taaaaagagg ctgggacaga
    12961 agcgtttaac tccaagaaat aagaaggagt tgcttctgct tccgccgttt atacgtgttt
    13021 agagcgtgag acaaaagtgt tctttacttt tgtctcacgc tctttttttg ttatattcgt
    13081 cttttccgat ccttctcttg acacaattca tttaactgat cactggtact tgaacaccta
    13141 atattctagt catttttata aacgattcaa tacttcatcc acatttttga tcatcacgga
    13201 tatcgtacca tccggattat tcacaaattc aatcaaatct gggttgcgat atacatctac
    13261 tggtacgatc agttcgatcc cgtttgatag cttgaatttt tgttttccaa attttttctc
    13321 agaaatttct tgtacttctt ttacaggagg tgcttctggg acaaatcctt gctcttgtac
    13381 ttcttcttta aaagccattt ttgccgtgat gttttccttg aagacttttt ccgcaatcat
    13441 ctcatgatcc aaccgacctt tttcttctat cgtatcatag acagcttctt tcaaatcagc
    13501 catgatatca aattcttctg tctcaaattt ttttccaaga tgcttggcta ctttttggat
    13561 cacttttaca ttttcttcta aagaaggtgc aggaacactt tcgatcactc ttccagaaaa
    13621 ataaagctct tttttgccag aaaactcata ttttttttca attaattcgt aggtccaatc
    13681 agtcaaattc acagcgaatg cttcatctgc tttttgtgtt ttcgccccta gaattgctcg
    13741 attcaaaatc aagcggttgt cgatccctgc ttcgtctgct tcaacaaagt gagtgtaagc
    13801 ctcacgataa ttgactttca ataaagataa gtacatcact gtatctaatt catacaagac
    13861 aacaaataca tctgcactag gtgcttcttc actttcttgg tagatatcga accaccgctg
    13921 aacgattgct tcacttgctt ccacaaaatt ttcttgagcg attttaagtg attcaacgat
    13981 catgctttct tctgctagtt gtccagtttt tgtttgagca gaagagattt tttggatctt
    14041 cttttgaaga aaatcacgaa tatattcttt tgtcagatcc agctcttttt gtgaaaagac
    14101 tggagaacca gtttctctgt caataatatg cagaattgct ttttttaaat agatatccat
    14161 tttctcattc ccttctgtct tagtttacgg aaaaaaaatc aaaaatacca gaatcaaaat
    14221 cattttcttt taaacaaaaa tttcaagagt agaaacaaaa tggattcttt ttttcgaaaa
    14281 atgcgctaca cttatagttg aacgaataaa aaaggagctg aaagtatgaa ttcattaaca
    14341 agcacttatc gtctatcaaa tggttacgaa atcccggtag taggattcgg cacatggcaa
    14401 acacctgatg gggatgtcgc tgtttcctct gttaaagaag cgctggctgc aggttatcgc
    14461 catatcgata ctgcccaagg atacaagaat gaagaaagcg tcggccaagc gattaaggaa
    14521 agtggaattc ctagagaaga gatattcttg acaacaaaat tatggaatgc aaaccacagc
    14581 tatgaacttg tgatgtcttc ttttgaagaa tcattgaaga agctccaaac agattattta
    14641 gatctgtttt tgatccactg gccaaaccct gttgcttttc gtgataattg ggaacaagca
    14701 aatgcagata catggcgagc atttgaagaa ctgtatgaag caggtaaaat caaagcaatc
    14761 ggtgtgagca atttcttacc ccaccatcta gatacactag caaaaacagc aaaaattatg
    14821 ccgatggtca atcaagtttt ccttgcacca ggcgaattac agacaactgt agttgagtat
    14881 gcaaaaaaac atgatatgat cctcgaagca tatagtccat taggcactgg taaaatcttc
    14941 gatgtgcctg aaatgaaaca aatcgctgaa gcacatgaca agacgattgc acaagttgct
    15001 ttgcgttggt cgctgcaaca cgaattcctt cctcttccta agtcagtgac accaagcaga
    15061 atcaaagaaa atactgaatt atttgatttt gaattaacag aagaagaaat gaaacaaatc
    15121 gatcagttag atggtgtagt cggcaaagca aaagacccag atactaccca attttaagtc
    15181 gaaaggtgat tttatggatc caatcgaacg cttgaattct ctctcggaag aagttaccca
    15241 gacttttcat tccgattttg tctttttgat cgatgcagag aaaatccaac attttcctgc
    15301 aagaaactgg acacatgatc aaatcatcga agaactgaaa aaacggttcg atcattcgct
    15361 aatggtcaaa ccctggcacg aacatgaagt catttattca cctgaactac ctgtttttgc
    15421 cttgataccg aaaaaataga aagaatggaa cggattatgt atatatacat aatccgttcc
    15481 attttttatt tttctttcaa aaaacgggaa aatacgcaaa tttttctgtt gtaatttgcg
    15541 tatacgcaag tttttttgca ttactccttt tcatttggct gtttttttct tataatcgaa
    15601 agggtatgag cgaaggagag aacatatgaa gaaaaaacag aaacaaaaat tattaaatca
    15661 attccgtcct tcattggatg gtgtaagagg acaattattc cgttctctag aagaagagag
    15721 cttgcgacgt tatggtttgc cgttacaagt catgcttgat cccaaaaacc gtcaaatctt
    15781 aacgatcgga ttagctggga aaacaaatga agatacacgg atcaacgtgc caatcgatga
    15841 caacttcaat actgtattaa agagaattcg ttcaggagaa aacgggattt tcgaacgatt
    15901 ccgtgacaat cttcttattg aaatcgtgtc ttactggaat gatcaacgat tgaaaaacga
    15961 tgaaccaact gcgcaagctg ttgcgacacc agttacagaa gaaacagtca gtgtgccaga
    16021 agaaaaaacg gaaaaaccaa aagcggacac aactgaaaat acacgctcaa ctgatacgaa
    16081 tgccgcttta agttttgctg atttttcaaa agccgttgct gagtatccta aattttacac
    16141 agaacaaaat gaacatgaag tagttattaa tgaaaaaaat gctgatgaac ctcgtaaatt
    16201 agcaactatt tctatgacta ctgaaaatga gttcgttatc gaaaaagcac tcgaacggaa
    16261 atacaaagtc aaattgactt tgattccttt gatcgaacaa tttgcagcga cagcgattac
    16321 tgatcgataa aaattctatt tgtcataaaa ggctgggatt taaaatccca gcctttttag
    16381 tcatataata atttctacag caagtaaaaa gaaattcctt ctaacatcag gctcaaatat
    16441 cagcttgacc tgtccaacaa atcacctatg atcctacaat gttaggcgac ttctcttcta
    16501 cctgaagata tcttggagtt gcctgatttc ttaagatgcc taaccttaac tataaaaaat
    16561 tctaataata aacatatttt ctgataga
//
LOCUS       DAZKLD010000058        16372 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.058, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000058 DAZKLD010000000
VERSION     DAZKLD010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16372
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.058"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(153..551)
                     /locus_tag="ACG8KR_001519"
     CDS             complement(153..551)
                     /locus_tag="ACG8KR_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HFQ0200887.1"
                     /translation="MKDLVALNLHMIVTIVDGGMGEDVIDFSKEAGAAGGTILHGRGS
                     GVHDSGKFLGLDISPEKDIVLTLVPDSLTNKVMDAIGQGINIDIPGNGICFSIDVDKV
                     IGITKIHQYREIIGMQELLDENNDREKSES"
     gene            complement(548..1321)
                     /locus_tag="ACG8KR_001520"
     CDS             complement(548..1321)
                     /locus_tag="ACG8KR_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HFQ0200888.1"
                     /translation="MSFWQQVIDGIEIVTMEVILAVAPIVILFVLMNIISFRMRKKRF
                     FDIMKGFLITTIGLILFLHGVNMAYVPVGQHLGSAIAGLPYNYILVPLGFLMGFLVGF
                     AEPAIHVMIKQVEELSEGRIRSKIMLMVISIGIGAAVGLSMWRLLVGFSLYYFLIPGY
                     GLAFILGRKVDKIFLAMAFDNGGVATGPMCSTFILSMSVAIAAQIEGRDPIIDGFGVV
                     AMIALTPILSTLVLGYIYKRKDEKDRIEKERLQKLGESK"
     gene            complement(1318..2001)
                     /locus_tag="ACG8KR_001521"
     CDS             complement(1318..2001)
                     /locus_tag="ACG8KR_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HFQ0200889.1"
                     /translation="MLKNFKEVIAAILPMTVLIVILTFVFAPLEGEDLASFLVGAAIM
                     MIGMTLFLFGADYSMMEVGDLVGKYMIKKKSLTVLVSLGFAIGIVITIAEPSVQVLGQ
                     QVYDISDGEIGRVLLIGIVSVGTGVFLAFALLRVVFKLSYYQLMAIGYIGVLIASFFT
                     SSEFMPIAFDSGGVTTGPVTVPFILALAGGMTSMIKQKKNENDSFGMVGIASLGPILA
                     VMILGVIFQ"
     gene            complement(2168..3055)
                     /locus_tag="ACG8KR_001522"
     CDS             complement(2168..3055)
                     /locus_tag="ACG8KR_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0200890.1"
                     /translation="MKNMVEKETIERPNVKKQALELRSTIGSVLKYTALSILSFISIF
                     PFVWMVLGMTNTPVDITSGKIKIGSNLIINFQNLFSNELNFTRSLWNSAMIALVTTVF
                     ALLISSMAGYGFEIYQSKRKDLIFNILLLSMMVPFAALMIPLYRMFSQLNGTVLGINS
                     FLVVILPSVSTAFLIFFFRQNVKSFPKDLVEAARLDGLKELSIFFRVYMPTAKNTYAA
                     AAIITFMSSWNNYLWPLVALQSPDKRTVPLVLSAMGASYTPDYGMIMAGLVIATLPTA
                     IVFFVLQKQFVQGMIGSVK"
     gene            complement(3052..3921)
                     /locus_tag="ACG8KR_001523"
     CDS             complement(3052..3921)
                     /locus_tag="ACG8KR_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0200891.1"
                     /translation="MKQKDLFKNGHAFLILPVILISIMVFIPMVLALITSFQSGPPVN
                     MTFNGLGNYQRMLTDATFKKAFFNTFLYLLVQVPIMLFLALFISNLLNDKKLKYKGLF
                     RTAIFLPCITSMVSYSLIMKSLFSQSGLVNNLLLGIGMIDTPIGWLTHPIWARVLIIL
                     SITWRWTGYNMIFFLSGMQNIDSEIYEAASIDGASKWQQFIKVTIPNLKPIILFTSIT
                     STIGTLQLFDEVQNITGGGPANATTTLSQYIYNLSYKFTPNFGYAAAVSFVIVSFIVI
                     LSIIQTKVGGESR"
     gene            complement(4058..5344)
                     /locus_tag="ACG8KR_001524"
     CDS             complement(4058..5344)
                     /locus_tag="ACG8KR_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFQ0200892.1"
                     /translation="MNIRKLIAGVVCLAAASGFLTGCGSASSTADKEEKVTVWAWDET
                     FNIKAVNEAKKVYENEETEIEVVTMSQDDIVQKLNTALASGNTEGLPNIVLIEDYRIQ
                     GYLTSYPDAFANLSAIVKEDHFAPYKFAVNKVGDKIYGVPFDSGVTANFYRTDLMAEA
                     GYTEEDMENLKWDEYIQVARDVKEKTGKKIAEVNPSDLGRVRMIMQQAGEWYTSEDGE
                     TVTIQDNASLKYGLELFATLLKEDLVEQTSDWNAGVTAIQSGAVASSPTGAWYSSTIQ
                     GAENQSGKWKIAPIPALPENLQKAQASNLGGAGWYVIKGVAGEDSAKDFLEATFASNE
                     ELMGTLAKEIGLVSTMLSANEQEAYQEASEFYSGQKIFDDFSTWTSEIPEVNYGHETY
                     AIEAVVAEALHRIINGEETDKVLADTQKQVEAQLAN"
     gene            5617..6195
                     /locus_tag="ACG8KR_001525"
     CDS             5617..6195
                     /locus_tag="ACG8KR_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFQ0200893.1"
                     /translation="MPIRVILIDDEQTILDGMKQLINWSEHGFQLIGTFSSPMKALDF
                     SLVHAIDIVVTDVMMPELNGIELSRLLKQQQPETQILILSSYDEFDMVKDSFKEGVAD
                     YLLKPRLSPDFFLNSLTAVSQKIKKKPNKSPITNRYEQICENLSQQIAGQTRPLLDPL
                     LFEHTLFFCYIPKNGHSVQNKALNKNSRSPIH"
     gene            6195..7124
                     /locus_tag="ACG8KR_001526"
     CDS             6195..7124
                     /locus_tag="ACG8KR_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0200894.1"
                     /translation="MDSDDSFTIYPVSTFSEESGYVINTDSKDTLRKFVQKFRQEVFP
                     GDFFVVSDSFAIQDINEIFQEIRNCTKGQRFYHKGKNLAYQHELLPLLTNHEQQASSY
                     LTKIVHKDYALAVKELENFFTTILEQPIDPAYLKHEAINRFYALLNALADEHVGNEEL
                     SLLKIALPSLLADAEYFEDFSILVKETLEKLTHFSANAKKDNFDILTLIYEFVQENYE
                     KEINLQLLSEKYHFSYSYLSAIFTEKFGINFSKYLKKVRISKAKLFLTETRLTLTEIC
                     EKVGYTELGYFSRVFKEETGMTPSQYRKGTFVK"
     gene            7121..8857
                     /locus_tag="ACG8KR_001527"
     CDS             7121..8857
                     /locus_tag="ACG8KR_001527"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFQ0200895.1"
                     /translation="MRKKIKEHELFAKLLFILFIGLFLQAIVISLFIYHRSRDAYIQL
                     FNQSNDVVLKKIQSEFESLNDTIENTLAAFDSNPAVKSYFSNDPAQHMEQLQQLRTIQ
                     KMNDSLSKIHPMIDYDVLIFGENGRTFVGNDMLTAVSADSFFQSAIAQRVNERAADTQ
                     MLFAHHGLTLRDKKAPSVFFVKKLKNTLNHVYGYAVLSISSKQLANLFQSVVNPEISK
                     ISFMNEEQTIIASNEENTIGSRSMIFKKLTVGETTISNGQQLTRLPLYRQDCTLLSQV
                     DLHSLSNQMGVIFPIILYNIVTLAFAGSLVFMYLDRHTKSIYRLIDSLKRLEEDGLKA
                     TVPVQGSHEVQLLGNTINQLLSNAQHHYEQTIQDEKKKRSLEIQTMQAQIQPHFIYNT
                     LTSLKFLIWQKKTDEAVQGIDSFITLLRSTIGKKEEVIPVKEEIKSVQSYIDLLSMRY
                     GERISAKIMIPDELQSLSMPNMILQPIIENAYLHAFHNKTQGFITVYGKTKNQLLLFE
                     VIDNGDGFDTENTKQKNDHFSGIGISHVDERIRLMYGDAYGLTIQSVIGTGTTVTICL
                     PLLPKNQSEYQK"
     gene            complement(8920..9756)
                     /gene="pheA"
                     /locus_tag="ACG8KR_001528"
     CDS             complement(8920..9756)
                     /gene="pheA"
                     /locus_tag="ACG8KR_001528"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HFQ0200896.1"
                     /translation="MNVSYLGPESSFTYQAACQLFPTEQLTAYASIPVCLRALFRKQV
                     DLAVVPVENSLEGAVHHTIDLLSKHPEVEVKSEIVLPIKQQLLGNPATKITKILSHPQ
                     ALAQSQQFLETHYPNVPLVATESTTAAAMYVAEHPKEDAAAIASLETAQHVGLEILAE
                     NIQDNELNQTRFWIVGDRKMTSQQSAPVKMSVILTLPANRPGMLHKMLAAFGWREINL
                     SKIESRPLKTSLGEYFFVIDLLLDRPMTLVENALQEIKMLGGESQILGCYPVLTVEET
                     RR"
     gene            complement(9753..10262)
                     /locus_tag="ACG8KR_001529"
     CDS             complement(9753..10262)
                     /locus_tag="ACG8KR_001529"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HFQ0200897.1"
                     /translation="MASILLIGFMGAGKTTIGKGLAQCLQKEYVDLDTKIEEHIQLSI
                     AEYFHYYGEKSFRKVESDILRKLSNEDKIIATGGGIVQSAENRRFLKTQPIVLYLEAE
                     ADCLVDRIQQDETSIRPLALGKTREEIKALLAQRLSWYEESATHRINTTNQTPEKIID
                     TIIERIKSI"
     gene            complement(10255..11550)
                     /gene="aroA"
                     /locus_tag="ACG8KR_001530"
     CDS             complement(10255..11550)
                     /gene="aroA"
                     /locus_tag="ACG8KR_001530"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HFQ0200898.1"
                     /translation="MQLLQQIHGLRGTVRIPADKSISHRSIMFGAIAEGTTTIQNFLR
                     AEDCLSTLHAFQQLGVEIEEEEEVIKIHGRGSHSFVQPTESIDMGNSGTTIRLLMGIL
                     AGQPFTTTLFGDASLSKRPMGRVMEPLREMGADLQGEENDQYLPITVTGTRSLSPIRY
                     NMPVASAQVKSALLFAALQAEGTSVIVEKERSRNHTEEMIRQFGGRITVEDKTIIVTG
                     PQKLTGQQITVPGDISSAAFFLAAGLLVPESQLLLKNVGVNPTRTGILDVLEEMGAAI
                     TQTNHNEQNQSADLSVKTSHLKKATIGGEIIPRLIDELPILALVATQAEGITIIKDAE
                     ELKVKETNRIDAVAEELQKMGAKIKATADGLIIHGPTPLHGAKVSSRGDHRIGMMLQV
                     AALIADGPCELEGAEAVSISYPAFFDDLAELVSGGEAHG"
     gene            complement(11563..12654)
                     /locus_tag="ACG8KR_001531"
     CDS             complement(11563..12654)
                     /locus_tag="ACG8KR_001531"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HFQ0200899.1"
                     /translation="MAQHVFIVGLGLIGASMALCIRQTNPELIVVGWDSQSKTREQAV
                     EQQIVDYVASDFETGAEQADVILLAVPVRTTLAYLDVLERINLSDHVIITDVSSTKQQ
                     VVTSAEQKNLRFVGGHPMAGSHKSGIQAADAKLFENAYYIFTPLEKTKKDVEKLQELF
                     CGTKAKYVTLTAREHDRITGMLSHFPHILAAGLVNQAEQFNQEYPRAKQLAAGGFRDI
                     TRIASSDPVMWTDILLSNKQILLERLADWQQEMTQIAEWIMTENQSEIFSFFNRAKES
                     RDQLPVHKQGAIPAFFDLFIDVPDEPGVIAEVTGLIGKAGVSLINLKIQETREDILGI
                     LQISFKNQQDLLQAKRIIMSNTSYHCWIK"
     gene            complement(12671..13837)
                     /gene="aroC"
                     /locus_tag="ACG8KR_001532"
     CDS             complement(12671..13837)
                     /gene="aroC"
                     /locus_tag="ACG8KR_001532"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HFQ0200900.1"
                     /translation="MRYMTAGESHGPELTAIIEGLPAGLPLSEEEINHELARRQAGYG
                     RGGRMKIEKDRVRITSGIRHGKTIGSPVTLVVENKDWKNWTTVMSVAEVPEKDQSLRR
                     VARPRPGHADLVGGMKYHHRDLRNVLERSSARETTMRVAIGAIAKKILEELDIIVAGH
                     VICLGGIQAEIPGNISIQEIKEISEASEVRVVDPAVEQRIKDLIDQTKKNGDTIGGIV
                     EVLVGGVPAGLGSYVQWDTKLDAKIAQAVVSINAFKGVEFGVGFTSGSLPGSEVMDEI
                     LWEEERGYFRRTNNLGGFEGGMTNGEPLVIRGVMKPIPTLYKPLQSVDIDTKEPYKAS
                     VERSDSTAVPAASVVAENVVATVVATELLSKFPSDSLVELKAAVKEYRDYLKHY"
     gene            complement(13839..14903)
                     /gene="aroB"
                     /locus_tag="ACG8KR_001533"
     CDS             complement(13839..14903)
                     /gene="aroB"
                     /locus_tag="ACG8KR_001533"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HFQ0200901.1"
                     /translation="MLTVHVDNQKYDIHIEKGALEQAGSWLQTIWRPQKVAVITDTNV
                     APLYAEKIMFQLRQANFEPVLSIVPAGETSKSLEQAAELYDFLADNDFTRSDGILALG
                     GGMVGDLAAFVASTFMRGIHFVQIPTTLLAQVDSSIGGKTAVNTNKAKNLVGTFSQPA
                     GVLIDPEVLKTLPVRRLREGIAEIIKSAAIADATLWKQLNELKDEKDLLEHAVPIITA
                     TLEIKRKVVEEDVFDQGNRLILNFGHTIGHAIENTAGYGVVSHGEGVAIGMVAITSHA
                     EKIGLSPTGSTAALERILQKFHLPITYDLSHPDELFHAITHDKKARGSQLNIILLEKI
                     GQAKIVPVPTETFKDYLGGL"
     gene            complement(14936..15958)
                     /gene="aroF"
                     /locus_tag="ACG8KR_001534"
     CDS             complement(14936..15958)
                     /gene="aroF"
                     /locus_tag="ACG8KR_001534"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HFQ0200902.1"
                     /translation="MIVIMKKEATEEQIKKVVDHIEKEGFKVSIDQGTERVVIGLKGD
                     TRSLQEGAFTRYEGVENAVRILNTYKLTSREFHPGNTVVDVDGVKIGDGSFVTMAGPC
                     SIEGLDQIRECARMAKAGGAKILRGGAFKPRTSPYAFQGLEEEGLKYIRQAADEFGMK
                     VITEVMDEGHIDMVAEYSDILQIGARNMQNFKLLSAVGKTGKPVGLKRGISGTINEWL
                     NAAEYIAVEDKSPVIFIERGIRTYETATRNTFDLSAVPLMKKLTHFPVIVDPSHGTGI
                     WELVPPMARAGVASGADGMIVEIHPDPANAWSDGPQSLNEKTYSRMMKEVAIMKEAME
                     KIQTLD"
BASE COUNT         4724 a         3620 c         2910 g         5118 t
ORIGIN      
        1 agcatatttt gaaaagaaag tctgatgctt agagtagaat ttcataaaaa tttactattt
       61 cataagtatt cacgagaaaa atgcaaacaa aaaagcggaa ataacgctca tccgccttct
      121 tgtttgtgtt tatttcctct tcaaacgctt gtctatgatt cagacttttc tctatcgtta
      181 ttttcatcta aaagttcttg cattccgata atttctcgat attgatgaat cttagtgata
      241 ccaatgactt tgtctacatc aatactaaaa caaataccat tacctggaat atcgatgttg
      301 atgccttgac caatcgcatc cattacttta ttcgtcaaac tatcgggtac taatgtcaac
      361 acaatatctt tttctggcga aatgtccaaa cctaaaaatt tccctgaatc atgtacacca
      421 gaacctcttc catgtaagat cgttccgcct gctgcacctg cttcttttga aaagtcgata
      481 acatcttcgc ccattcctcc gtcaacgatc gtcacaatca tgtgaagatt caatgctaca
      541 agatctttca tttgctttcc cccaattttt gcaatctttc tttttcgatt ctgtcttttt
      601 catcttttcg tttataaata tatcctagta ctaaagtgga taatattggt gtcaatgcaa
      661 tcattgcaac cacaccaaac ccgtcaatga tcggatctct tccttcaatt tgtgcagcga
      721 ttgccacgct cattgataaa ataaatgtcg aacacatcgg gcccgttgct actccaccgt
      781 tatcaaaagc cattgctaaa aagatcttgt ccacttttcg tcctaaaata aatgctagcc
      841 cgtatcctgg gatcaagaaa taataaagag agaatccgac gagcaaacgc cacatagaca
      901 atcctactgc tgcgccgatc ccaattgaaa ttaccatcag catgattttc gatcggattc
      961 tcccctcgct caattcttca acctgtttga tcattacatg aatcgccggc tcagcaaacc
     1021 ctactaagaa ccccattaaa aagccaaggg gaaccagtat ataattgtaa ggcaatcccg
     1081 caatagcact gcctaaatgc tgtcctaccg gtacataagc catattgact ccgtgcagga
     1141 aaaggatcaa cccaatagtc gttatcaaga aacctttcat aatgtcaaag aaacgctttt
     1201 tgcgcatacg aaaactaatg atattcatta aaacaaatag gattacgata ggtgccactg
     1261 ccaaaatgac ttccatggta acgatttcga ttccatctat cacttgctgc cagaaactca
     1321 ttggaagatc acccccaaaa tcatcacggc aagaatcggc cctaacgaag caataccgac
     1381 cattccaaaa ctgtcatttt catttttctt ttgtttgatc atactagtca tccctccggc
     1441 aagggccaag ataaatggaa cagttactgg accagtagtc actccccccg aatcaaaagc
     1501 aatcggcata aattcggaac tggtgaaaaa tgaggcgatc aaaacgccta tgtagcctat
     1561 agccatcaat tgataatagg atagcttgaa tacgacccga agcagcgcaa aggctagaaa
     1621 tactccagtg ccgacgctga cgatcccaat cagcaacact ctgccaatct ctccgtcaga
     1681 tatatcatat acttgttgtc ccaaaacttg cacggaaggt tcagcaatcg tgatcacgat
     1741 cccgatagca aatcccaaac tgaccaaaac ggtcaaactt ttctttttga tcatatattt
     1801 ccctactaga tctccgactt ccatcataga ataatctgct ccaaacaaaa agagcgtcat
     1861 tccgatcatc atgattgccg caccaaccag aaatgaagcg aggtcttctc cttctaacgg
     1921 tgcaaaaaca aacgttaaaa tgacaatcag gacagtcatc ggcaatatag cagcaatcac
     1981 ttctttaaaa ttttttagca tgtatcctcc tcctttactc tttaagttta ccttaaaatg
     2041 aaagagtttt cgagcacaaa cttaaaacgt tatcatttaa tttttctcaa cataaaataa
     2101 cacacgtccc aaaagtatct ttttacttgg aaaaatgact ttgaaggaac gcgcttattg
     2161 tgttttttta ttttacagag ccgatcattc cttgaacgaa ttgcttttgc aaaacaaaaa
     2221 atacgatggc agttggtaaa gtagcgatga ctaatccagc catgatcatt ccatagtctg
     2281 gtgtatagga agcacccata gcagataaaa caagcggaac cgtccgttta tcaggagatt
     2341 gcaaagctac taagggccat aaataattgt tccagctact catgaatgtg atgattgcag
     2401 ccgcggcata ggtattttta gccgtcggca tatacactct aaaaaaaata cttagttctt
     2461 ttagaccgtc taatcgagcg gcttctacta gatcttttgg aaaagattta acattttgtc
     2521 gaaagaagaa gatcaaaaaa gcagtgctaa cagatggcag gatgacaacc aagaaactgt
     2581 tgattcccaa aaccgttccg ttcaattgtg aaaacattcg atataaaggg atcatcaatg
     2641 ccgcaaaagg aaccatcatc gatagcagta gaatattgaa aattaggtct tttcgtttac
     2701 tttgataaat ctcaaatcca tatcctgcca ttgacgaaat cagcaatgca aacacagtgg
     2761 tcaccaaagc gatcatcgct gaattccaaa gggaacgagt aaaattcagc tcattggaaa
     2821 acaaattttg aaaattgatg atcaagttac tgccaatctt tatttttccc gatgtgatat
     2881 ccacgggtgt attcgtcatt cccaaaacca tccagacaaa agggaaaatg gaaataaatg
     2941 ataggatgct taatgctgta tactttaata ctgacccaat tgtcgaacga agttctaatg
     3001 cttgtttttt gacgttgggg cgttcgattg tttctttttc taccatattt ttcatcgact
     3061 ttctcctcct actttcgttt ggattatcga caatatcaca ataaaggaaa caataacaaa
     3121 ggaaactgct gccgcatatc caaaattagg cgtgaattta taactcaaat tatagatgta
     3181 ttgagatagg gtggtcgttg catttgctgg tcccccgcca gtaatatttt gtacttcatc
     3241 aaaaagctgc aatgtcccga tcgtcgaagt aatggaagta aacaaaatga ttggttttaa
     3301 atttgggatc gtcactttga taaactgctg ccatttagaa gcaccgtcaa tagatgctgc
     3361 ttcataaatc tccgaatcaa tattttgcat gcctgaaagg aaaaaaatca tattgtatcc
     3421 agtccaacgc caagtgatcg aaagaatgat cagaactctc gcccaaattg gatgagtcaa
     3481 ccagccaatc ggtgtgtcaa tcataccaat gcccaacaac aagttgttta caaggccaga
     3541 ttgagaaaat agacttttca tgatcagaga ataactgacc atcgaagtga tacatggaag
     3601 aaagatagcg gttcgaaaca accctttata tttcaacttt ttatcattta aaaggttgga
     3661 aataaaaagt gctagaaaca acatgatcgg tacttgtaca agtaaataca ggaatgtatt
     3721 aaaaaaagct tttttaaaag ttgcatctgt caacatccgc tgataattac ctaacccgtt
     3781 aaatgtcata ttgacagggg gaccactttg aaaagacgtg atcaaagcca aaaccatcgg
     3841 aataaaaacc atgattgata tcaaaatgac tggcaagatt aaaaaagcat gcccgttttt
     3901 gaataaatct ttctgcttca ttcttttccc tccaattcta tcattgatta tctacatgaa
     3961 aagacaagaa agacaatatc tgtaaggcag acgtgatctt ttttgtcttt tcgtttgtct
     4021 gatgaaaaag cgaagaaatt cactttttta ccaccgatca attagccaat tgtgcttcta
     4081 cttgtttttg tgtatctgct agtaccttgt ctgtctcttc tccattgatg attcgatgaa
     4141 gagcttcagc tacgactgct tcaattgcat aggtctcatg tccgtaattc acttctggta
     4201 tttcagatgt ccatgttgaa aaatcgtcaa agatcttttg accactgtaa aattcagaag
     4261 cttcttgata agcttcttgt tcattcgcgc taagcatcgt agatactagt ccgatttctt
     4321 tcgcaagagt gcccatcagt tcttcattcg aagcaaacgt tgcttctagg aaatctttcg
     4381 ctgaatcttc tccagctact cctttgatca cataccagcc agcgccgcca aggttagaag
     4441 cttgcgcttt ttgcagattt tctggaagtg caggtattgg tgcaatcttc cattttccag
     4501 attggttttc tgctccttgg atcgttgaag aataccaagc gccagttggg ctagaagcta
     4561 ctgctcctga ttgtatcgca gttactccag cattccagtc agaggtttgt tcgactagat
     4621 cttctttcaa aagagtggca aacaattcaa gaccatactt taaagatgca ttgtcttgga
     4681 tcgtgactgt ctccccatct tcagatgtat accattctcc tgcctgttgc ataatcatcc
     4741 ttactcgacc aaggtcagaa ggattgactt cagcgatttt ctttcccgtc ttttctttga
     4801 catctcgcgc tacttgaata tactcgtccc acttcagatt ctccatatcc tcttctgtat
     4861 aacctgcttc tgccatcaga tccgtacgat agaagtttgc tgttacgccc gaatcgaaag
     4921 gaacaccgta tattttatct cctactttgt ttaccgcaaa tttatacggt gcaaaatgat
     4981 cttcctttac aatagcagac aaattagcga atgcatctgg ataagaagtc aaatagcctt
     5041 gtatcctata atcttcaatc aagacgatat tgggcaatcc ttctgtattc ccgctcgcta
     5101 aagcggtgtt cagtttctga acgatatcat cctgagacat cgtaacaact tctatctctg
     5161 tttcttcgtt ctcataaact ttttttgctt cattcactgc tttgatattg aatgtctcat
     5221 cccacgccca gacagtgact ttttcttctt tgtctgctgt actactagca cttccgcatc
     5281 ctgttaagaa acctgatgct gccgctaaac aaacgactcc agcaattaac tttctgatgt
     5341 tcatttttat ccgctccttt tccatccatt agttgtttac gtttgctaca ttcatcatac
     5401 caaaatttga aaacggtttc tttattattc agatacttca aaaatttctc ctaaaataat
     5461 aaaaaaatac aaatcaacta tgacaaaagg aataatctta gcatctagac caaagatcga
     5521 aatggtgagg tttctttaaa ataaaaatat ccttgcattt tctcctagaa ataagtattt
     5581 ttaaattaca agactattct taaggcgggt aaaaaaatgc caatacgcgt aattcttatc
     5641 gatgatgaac agacgatttt agatggaatg aaacagttga tcaattggtc tgagcacggg
     5701 tttcaattaa tagggacttt ttccagtccg atgaaagcgc ttgatttttc tttggtacat
     5761 gcaatcgata tcgtggtcac agatgtaatg atgcctgaat taaatggaat cgaattatcg
     5821 cggctattaa aacagcaaca acctgaaaca cagatcctta tcctctctag ctatgatgaa
     5881 tttgatatgg ttaaagattc ttttaaagaa ggcgtcgctg attatctttt aaagcctcga
     5941 ttgagtcctg atttttttct aaattcctta actgccgttt cacaaaaaat caaaaaaaag
     6001 cccaacaaaa gcccaatcac caatcgttat gagcagatct gcgagaatct ttcacagcag
     6061 attgctggac aaactcgtcc attgcttgac cctcttcttt ttgaacacac cttgtttttc
     6121 tgttatatac cgaaaaacgg ccattcagtt caaaacaagg ctctaaacaa aaacagtcga
     6181 tcacccattc attgatggat agcgacgatt cgtttactat ttatcctgtt tctacctttt
     6241 cggaagaatc cggctatgta atcaatactg attccaaaga tacacttagg aagtttgttc
     6301 aaaagtttcg tcaagaagtt tttccaggtg atttttttgt tgtttctgat tcttttgcaa
     6361 tccaagatat caatgaaata ttccaagaga tcagaaattg caccaaagga caacgtttct
     6421 atcataaggg aaagaacctt gcttatcagc atgaacttct tccgttatta accaaccacg
     6481 agcagcaagc aagtagttat ttaacaaaaa tcgttcataa agactacgct ttagctgtca
     6541 aagaacttga gaatttcttc acgacgatat tggaacagcc tattgatcca gcttatttga
     6601 aacatgaggc aatcaatcgt ttctatgcat tactcaacgc tttggctgac gagcatgttg
     6661 ggaatgaaga actttctttg ctcaagatcg cccttccttc tttattagcc gatgctgagt
     6721 atttcgaaga tttttctatt cttgtcaaag aaacacttga gaaactcacc cacttttctg
     6781 caaatgcaaa aaaagacaat ttcgatatct tgactttgat ctacgagttc gtccaggaaa
     6841 attatgagaa agaaatcaat ttacaacttt tatccgaaaa atatcatttt tcatatagct
     6901 atctctcagc catctttact gaaaaatttg gtatcaactt ttcaaaatat ctaaaaaaag
     6961 tccgtatcag taaagccaag ctctttttga cggaaacacg gcttactttg acggaaatct
     7021 gtgagaaagt cggctatacc gaactgggtt atttctccag agtttttaaa gaagagacag
     7081 gaatgacacc ttctcaatac cgtaaaggaa cttttgtaaa atgagaaaaa aaatcaaaga
     7141 gcatgaactc tttgccaaac ttctgttcat tttgttcatc ggtctttttc tgcaagccat
     7201 cgtcatttct ttgtttattt atcatcgatc aagggatgcc tatatccagc tgttcaatca
     7261 atcgaatgat gtggtactaa aaaagattca atctgaattt gaaagtttga atgatacaat
     7321 cgagaataca ttagccgctt ttgattctaa tccagctgtc aaaagctatt tttccaatga
     7381 tccagcccaa catatggaac agcttcagca actaagaaca atccaaaaaa tgaatgactc
     7441 tctttcaaaa atccatccaa tgatcgatta cgatgtattg atctttggag aaaatggaag
     7501 aacttttgtc ggaaacgaca tgctcactgc cgtatctgcc gattcttttt ttcaatctgc
     7561 gattgctcag agagtgaatg aacgtgctgc tgatacacaa atgctttttg cacaccacgg
     7621 attgactctt cgcgataaga aagcacccag cgtcttcttt gtcaaaaaac tgaaaaatac
     7681 attgaatcac gtttacggat atgctgtttt atcgatctca tccaagcaac tagcgaatct
     7741 ttttcaatct gtagtaaatc ctgagatatc gaagatctcc ttcatgaacg aagaacagac
     7801 gatcatcgca tcaaatgaag aaaatacaat tgggagccga tcaatgattt tcaaaaaact
     7861 aacagtaggt gaaacaacga tttcaaatgg acagcagttg acccgtcttc ctctgtaccg
     7921 ccaggattgt acattactca gtcaagtcga tcttcattct ttatctaacc aaatgggcgt
     7981 gatttttccg attattttgt ataatatcgt gacacttgct tttgctggct cattggtatt
     8041 tatgtatttg gatcgacaca caaaatcgat ctaccgcttg attgattcgc taaaacggct
     8101 agaggaagat ggcttaaaag ctactgttcc tgtacaagga agccatgaag tccaattgct
     8161 agggaatacg atcaatcaat tgctaagtaa tgctcagcat cattatgaac agacgatcca
     8221 agacgaaaag aagaaacgtt cgcttgaaat ccaaactatg caagcacaga tacagcccca
     8281 ttttatttat aatacgctga ctagcttgaa atttttgatt tggcagaaaa aaacagatga
     8341 agcagttcaa ggaatcgaca gtttcattac tttactaaga agtacgattg ggaaaaaaga
     8401 agaagtgatt ccagtcaagg aagaaatcaa aagcgtacaa agttacatcg atcttttgag
     8461 tatgcgatat ggcgaaagaa tctcggctaa aataatgatt cctgatgaac tgcagtcatt
     8521 atctatgccg aatatgatcc ttcaaccaat tatcgaaaac gcctatcttc atgcttttca
     8581 caataaaacg caaggattca tcacggtgta cggcaaaaca aaaaatcagc ttcttttatt
     8641 cgaagtgatt gataacggcg atggcttcga tactgaaaat accaaacaaa aaaatgatca
     8701 tttttcagga atcggcatca gccatgtaga tgagcggata cgcctaatgt atggcgatgc
     8761 atatggctta acgatccaat cagtgattgg gacaggaacg actgtaacga tttgtctacc
     8821 gcttcttcct aaaaatcaat cagaatatca aaaataaaga atagccaagc gctgattttt
     8881 ttattttaag ttaaataaat caatcagtat tcttgacttt catctacgcg tctcttctac
     8941 tgtcagcact ggataacagc ccaatatttg actttctcct ccgagcatct tgatttcttg
     9001 gagcgcattc tctactaatg tcattggacg gtccaacaac aaatcgatca cgaaaaaata
     9061 ttctcccaag ctagttttca atggtcggga ttcaatctta cttaagttga tttcccgcca
     9121 gccaaaagct gccagcattt tatgaagcat ccctggccga ttagcaggta gtgtcaagat
     9181 gacactcatt tttactggag ctgactgctg gctggtcatc tttcggtcac cgacgatcca
     9241 aaaacgtgtc tggttcaact cattatcttg gatattctca gctaagatct ccaatccaac
     9301 atgctgtgct gtttctaatg aagcaatcgc tgctgcgtct tctttcggat gttctgctac
     9361 atacattgct gcagcagttg ttgattctgt tgctactaaa ggaacattag gataatgcgt
     9421 ttctaaaaac tgttgtgatt gtgctagtgc ttgtggatgt gaaaggattt ttgtaatctt
     9481 tgttgctgga ttgcctaaaa gctgctgctt gataggaaga acgatttcac ttttaacttc
     9541 gacttctgga tgtttggaaa gaagatcgat tgtgtgatga acagctcctt ccaatgaatt
     9601 ctctacagga acgacagcca gatcgacttg tttgcgaaac aaagcgcgca aacaaacagg
     9661 gatggaagca tacgcagtca attgttctgt aggaaatagc tgacaagccg cctgataagt
     9721 gaatgaactt tctggcccta gatagctgac gttcatatgc ttttgatcct ttctataatg
     9781 gtatcgatga tcttttctgg tgtttgattg gtcgtgttga ttcgatgtgt cgcactttct
     9841 tcataccaag aaagtcgttg tgccaataat gcttttattt cttctcttgt ttttccaaga
     9901 gctaaaggtc gaatagacgt ttcatcttgt tggatacgat caactagaca gtccgcttct
     9961 gcctctaagt aaagaacaat cggctgtgtt tttaaaaaac gccgattttc tgcactttgg
    10021 acaatccctc caccagtcgc gataattttg tcttcatttg acagctttct taaaatatct
    10081 gactccactt ttcgaaagct tttttcaccg taatagtgaa aatattcggc aatcgacaat
    10141 tggatatgtt cctcaatctt tgtatctaaa tctacatact ctttttgtaa acattgagct
    10201 aaccctttac cgattgttgt ttttccagcg cccatgaaac caatcagcaa aatgctagcc
    10261 atgagcttct cctcctgaca caagttctgc taaatcgtca aaaaatgccg gataagagat
    10321 tgaaacagct tcagctcctt ctagttcgca aggtccatca gctatcaaag cagctacttg
    10381 cagcatcata ccgattcgat gatcgcctct gctggacact tttgcgccat gcagaggtgt
    10441 cggcccatgg atgatcagac cgtctgctgt cgcttttatc tttgctccca ttttctgtaa
    10501 ttcttctgcc accgcatcaa ttctgttggt ttcttttact tttaattctt ctgcgtcttt
    10561 gatgatcgtg attccttctg cttgagtagc cactaaagct agaatgggca attcatcaat
    10621 cagacgcgga atgatttctc caccaatcgt tgctttcttc aaatgactcg tcttgacact
    10681 taaatcagcc gattggttct gttcattgtg attcgtctgg gtaatcgctg cgcccatctc
    10741 ctccaacaca tctaagatac cagttcttgt tggattgacc ccgacatttt ttaacaacag
    10801 ttggctttcc ggaacaagaa gtcctgctgc tagaaagaaa gcagctgatg aaatatcacc
    10861 tggaacagtt atctgttgac cagttaattt ttgcggtccg gtcactatga tcgttttatc
    10921 ttccactgtg atccttccgc caaattgacg aatcatttct tccgtatggt tacgggaacg
    10981 ttctttttca acgattacgg atgtgccttc tgcttgtaat gccgcaaaca gcaaagcaga
    11041 tttgacttgc gcactggcta caggcatatt gtatcggata ggtgataaag agcgggttcc
    11101 tgtcacagtg attggtagat actggtcatt ttcttctcct tgcaagtcag cgcccatctc
    11161 gcgtaaaggt tccatcactc gccccattgg acgtttagac aacgaagcat ccccgaacag
    11221 agtagtcgta aaaggctgtc cagccaaaat acccatcaat aaacgtatgg tcgtaccgga
    11281 gtttcccatg tcaattgatt cggttggttg gacaaatgag tggctaccgc gaccatgaat
    11341 cttgatcacc tcttcctctt cttcgatctc gacacctaat tgttggaagg catgtaaagt
    11401 actcagacaa tcttctgcgc gcaaaaaatt ttgtatagtc gtcgttcctt cagcaattgc
    11461 tccaaacatg atgctgcgat gggaaattga tttatctgct ggtatcctaa cagtccctct
    11521 taatccatgt atttgttgta ataattgcat ttatccagcc tcctatttga tccagcaatg
    11581 ataactggta ttagacataa tgattctttt tgcttgtaac aaatcctgtt ggtttttaaa
    11641 actgatttgc agtattccta aaatatcttc cctggtctct tggatcttca aattaatcaa
    11701 agaaactccc gcttttccga tcagtccagt tacttctgca ataacgccag gttcatctgg
    11761 cacatcgata aataaatcaa aaaaggcagg aatcgcgcct tgcttatgga caggcaactg
    11821 gtctcgactt tcttttgccc gattgaaaaa agaaaagatt tccgattggt tctcagtcat
    11881 gatccattct gctatttgtg tcatttcttg ttgccaatct gccaatcgtt ccaataatat
    11941 ctgcttattg ctcaacaaaa tatctgtcca catgacagga tctgaggaag cgatcctagt
    12001 gatatctcga aagcctcctg ctgccagttg tttggctcgc ggatactctt gattgaattg
    12061 ttctgcctga ttgactaagc cagctgccag aatatgtgga aaatgactta gcattcctgt
    12121 tatacggtca tgttctcttg ctgtaagagt tacatatttt gctttggttc cgcaaaatag
    12181 ttcttgcagt ttttcaacat cttttttcgt cttttccaaa ggtgtaaaga tgtaataggc
    12241 attttcaaat agtttagcat ctgctgcctg tatacctgat ttatgagaac cagccattgg
    12301 atgtccaccg acaaaccgaa gatttttctg ctcagcggat gtcacgactt gctgcttcgt
    12361 actgctgaca tccgtaatga tgacgtgatc agacaagttg attctttcaa ggacatcaag
    12421 ataagctaag gttgtacgga cgggaacggc taataagata acgtctgctt gttctgcacc
    12481 tgtttcaaaa tcacttgcaa cgtaatcaac gatctgctgt tctactgctt gctcacgggt
    12541 ttttgactga ctatcccaac caactactat cagttctgga ttggtctgac gaatacaaag
    12601 tgccatagaa gcaccaatca gtccaagccc cacgataaaa acatgttgtg ccatcttatt
    12661 tgcccccttt ttagtaatgt ttcaagtagt cacggtactc cttcactgca gctttcaact
    12721 caacaagcga atcacttggg aattttgaca atagctctgt cgctaccaca gtggctacaa
    12781 cattttccgc aacgacgcta gctgcaggaa ctgctgtact atccgaacgt tctacacttg
    12841 ctttgtaagg ttcttttgta tcgatatcaa cactttgaag tggcttgtac aatgtaggaa
    12901 tgggtttcat gaccccgcga atgacaagtg gttcgccatt ggtcattccg ccttcgaagc
    12961 cgcctagatt attggtacgt cggaaatagc ctctttcttc ttcccatagg atctcatcca
    13021 tcacttctga gcctggtaat gaaccagaag tgaatccgac cccaaattct acgcctttaa
    13081 aggcattgat actaaccaca gcttgtgcga ttttggcatc tagcttcgtg tcccattgta
    13141 cgtagcttcc taatccagct ggaacgcctc caacgagcac ttctacaatt cctccgatcg
    13201 tatctccatt tttctttgtc tgatcgatca aatcttttat tctttgctca actgctggat
    13261 caacaactcg tacttctgac gcttcagaaa tttctttgat ttcttgaatc gagatatttc
    13321 caggtatttc tgcttggatt ccaccaagac aaatcacgtg tcctgctacg atgatgtcaa
    13381 gttcttccag aattttcttg gcaattgcgc cgattgctac acgcatggtc gtctctcttg
    13441 cagaggaccg ttcaagcaca ttccgaagat ctcgatgatg gtatttcata ccgcctacca
    13501 aatccgcgtg tcctggacgc ggacgtgcaa ctcgccgcag tgattggtct ttttcaggca
    13561 cttctgctac agacatgaca gttgtccaat ttttccaatc tttattttct accactaaag
    13621 tgacaggtga accaattgtt ttgccgtgac ggatgccaga cgtgatccga acacgatctt
    13681 tttcgatctt catccttcct cctctgccgt aaccagcttg tctgcgagcg agttcgtgat
    13741 tgatttcttc ttctgacaga ggtaatcctg ctggtagtcc ttcaataatg gctgttaatt
    13801 ctggtccatg ggattctccc gcagtcatat aacgcatctt acagtcctcc taagtaatct
    13861 ttgaatgtct ctgtgggaac aggaacgatt tttgcttgcc caatcttttc tagcaagatg
    13921 atattcaatt ggcttcctct tgctttctta tcatgggtta tcgcgtggaa taattcatct
    13981 ggatgactta gatcgtacgt aatcggcaga tggaattttt gaagtatcct ttctaaagca
    14041 gctgtcgatc cagttggaga caagccgatt ttttctgcat gagaagtgat ggctaccatt
    14101 ccaatagcta ctccttctcc atgactgact accccatatc ctgctgtatt ttcaattgca
    14161 tggccaattg tatgaccaaa atttaaaata agtcgatttc cttggtcaaa gacatcttct
    14221 tccactactt ttcgtttgat ttccaacgta gcagtaatga ttggaacagc atgttccaga
    14281 agatcttttt cgtctttcag ctcgttcagc tgtttccaaa gcgttgcatc tgctatagct
    14341 gctgatttga tgatttcagc gattccttct cgtaaacgac gtaccggcaa agtttttaga
    14401 acctcaggat caatcaatac accagctggc tggctaaatg ttccgacaag gtttttcgct
    14461 ttgtttgtat tgactgctgt cttcccgcca atactagaat ctacttgtgc aagtagcgta
    14521 gtcggaattt gaacgaaatg gatcccacgc atgaacgtcg aggccacgaa tgctgctaga
    14581 tcacccacca ttcctccacc aagtgcaagt atcccatcgc tgcgggtaaa atcattatct
    14641 gctaaaaaat cgtataactc agctgcttgt tctaaagatt tactggtctc acccgccggt
    14701 acgattgaaa ggactggttc aaaattagct tgtctcaatt ggaacatgat tttttctgca
    14761 tatagtggag caacgtttgt atcagtgatc acagctactt tttgaggtct ccaaatcgtt
    14821 tgaagccatg atccagcctg ttctagtgcg cctttttcaa tatgaatatc gtatttttgg
    14881 ttatcaacat gtacagtaag cattcgtttc acttctttct tttaaaaagc tggaattaat
    14941 cgagtgtctg aatcttttcc attgcttctt tcatgatcgc aacttctttc atcatgcgtg
    15001 agtaagtctt ttcattcaac gattggggac cgtctgacca agcatttgca ggatcaggat
    15061 ggatctctac gatcatgcca tctgctccgc ttgccacacc cgcacgagcc attggtggta
    15121 caagttccca aattcctgta ccatgacttg gatcaacaat cacagggaaa tgagttaatt
    15181 ttttcatcaa tgggacagca cttaaatcaa acgtattacg tgtcgctgtt tcgtaagtcc
    15241 gaatcccacg ttcgataaaa atgacaggag atttgtcttc gactgcaatg tattctgcag
    15301 cattaagcca ttcattgatc gtaccggaaa ttccacgttt caatccaaca ggtttacctg
    15361 ttttaccgac agcagatagc agtttaaaat tttgcatatt acgtgcgcca atctgtagga
    15421 tatcgctgta ctcagccacc atatcgatat gcccttcatc catcacttca gtaatcactt
    15481 tcattccaaa ttcatcagcc gcttgacgga tgtatttcaa tccttcttct tctaaacctt
    15541 ggaaagcata tggagaagta cgtggtttga aggcaccacc tcgcagaatt tttgcgcccc
    15601 cggctttggc cattcgcgca cattctctga tttggtctaa tccttcgata gaacaaggac
    15661 cagccatcgt cacgaaactg ccgtcaccaa tttttactcc gtctacatcc acgactgtat
    15721 tgccaggatg gaattctcga gaagtcaatt tgtatgtgtt taaaatacgc acagcatttt
    15781 ctactccttc atagcgtgta aatgctcctt cttgtaaact tcgcgtatca ccttttagtc
    15841 caatcactac tcgttctgtt ccttgatcga ttgagacttt gaatccttct ttttcgatat
    15901 gatcgacgac tttttttatt tgttcttccg ttgcttcttt tttcataatt actatcattg
    15961 tttttcccgc tttcttttgt ttacttatct atcttggctg attttctcta ctttttcaag
    16021 gtggttttac attaattcct actaaatgac cgtgtcctgt tgttctcatt ttcgctcctt
    16081 ctttcctact tatgacccag ataatcaaca aaaaaagatg tccctgtagg aaattgcctc
    16141 tacagtgaac atcttcaacg agccttaaaa agcgctgcaa aaatataaaa tagccactat
    16201 agatgaattt ctctacgtgg ctgtaagtcg tgtttaaaaa tctttagcca tcatagatac
    16261 aaacctggtt tgtttgtacc cacgaagtca tgaatacaaa cctatctaaa atagctaaag
    16321 taatacgtat tcagttttgt aagttttcta gatttcataa aaaatcgctc ca
//
LOCUS       DAZKLD010000059        16187 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.059, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000059 DAZKLD010000000
VERSION     DAZKLD010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16187)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16187)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16187
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.059"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            43..1470
                     /locus_tag="ACG8KR_001535"
     CDS             43..1470
                     /locus_tag="ACG8KR_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0200903.1"
                     /translation="MIEEYIEKNILRQLFLCGQFYVNKEVNLEKLSNLLHVCKTTLLN
                     DINNIKKEFEEQIAYTHREKDRYTLYFSEHIPRCKIMQQLSQNSLFLKTCLLYLEEDE
                     PDYLQLTECEFISVSKAYSLKKQVLAYFNDCGIEIDRYSPRFTEMERRLLLLNVSYRL
                     GGFNSWELPESFFERADRFIESVTENSGRFYDKENKEILSIGFAISFLRQQVCAVTID
                     SKFIEEIKKRPIYNYVESAWENTDFQTYYKKEEFAFILTLFNLCNYGFHSYQLIAEDF
                     QQLHQVFIDNTPEIKELVATFESHFNQELFGNQPFERALIHLMRSAWDNYQLFMPEKF
                     YLLNEEQTNLYKEVQTIFSSWSSQLPYDLRLNPNCMRAFVIELSGILRLTKEHLTIYI
                     VTNSDVHYLIYREALEAVTTFDFQVAPTIYSSISDIKKYAQQSSNRVLCERTLYTPDA
                     VQYENIIPISINTIDRAIISAVQNK"
     gene            complement(1562..1927)
                     /locus_tag="ACG8KR_001536"
     CDS             complement(1562..1927)
                     /locus_tag="ACG8KR_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HFQ0200904.1"
                     /translation="MTTELMQFLLPYFEKLVLGLDIFAICLLVWGVCLSIKDFLKYLF
                     VHTKVQSDRVSENNAIKKMLGGYILLSLEILVSAGIIESIIKPTLQDIFQLAALVVIP
                     TIISYFLNKEIGPVDVQNK"
     gene            complement(1924..2289)
                     /locus_tag="ACG8KR_001537"
     CDS             complement(1924..2289)
                     /locus_tag="ACG8KR_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HFQ0200905.1"
                     /translation="MFRSNLIEFLEWIIAILNICSIGILIVGIVLVLFKLFDWSHVKK
                     NYAERNTRNADTRKLLASYILLSLEVLVVADIIESVIKPTWTDILKLALIIIIRTVIS
                     YFLNQEIEDKEKQRRGEVG"
     gene            complement(2481..2729)
                     /locus_tag="ACG8KR_001538"
     CDS             complement(2481..2729)
                     /locus_tag="ACG8KR_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0200906.1"
                     /translation="MKNASLFSLSISKDSSFLLECQMIGREEKGNFDKKEEVSDFPVN
                     KNRVGKKKRFEEVYGSIFRNLGTKKAPRQCFFEKLMTD"
     gene            2728..2856
                     /locus_tag="ACG8KR_001539"
     CDS             2728..2856
                     /locus_tag="ACG8KR_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanol dehydrogenase"
                     /protein_id="HFQ0200907.1"
                     /translation="MSKKLIKVGIGLGLLALGAAYLGKKTGLFEDDSHLYDEFESI"
     gene            2913..4607
                     /locus_tag="ACG8KR_001540"
     CDS             2913..4607
                     /locus_tag="ACG8KR_001540"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050151 - oleate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HFQ0200908.1"
                     /translation="MRKSKAIMIGAGIANMAAAVYLIQEGKWKGDQITFYSLDDHGSN
                     DGAPTDTVVDEYWNKNHPLENQKGYIARGGRMLNYRTYVDLMDLLSRIPSVTEPGMTA
                     EEDTRDFDAKHRTFDKARLMEGGKGIIDGGKLGFNNKDRILLTKLIAMPDSEEEKLDN
                     ITIEEYFKDDPHFFETNFWFMWETTFAFRTRSSAQELRRYMHQMIYEFTQIEHLVGVN
                     RTRYNQYESIMLPLIEYLKEQGCKIILNRRVTDWEFKETPMQDEITVTGLKITNTLTQ
                     EEEYVVVDEDTAVIFTNGSITDSATLGDYETPAVENMDYGAASSLWKKASERFYNLGN
                     PDKFFADRDASEWVSFTLTTKNHLLLNEITRITTQEPGNALNSFISTTPITPLGQKDV
                     NMSIVVHHQPHFTSQKPNETVIWGYFLYPRRRGEFVDKQYIKMNGKEMLEELIGQLSK
                     VDPGPVNIREKETEIFDSVINNIPVYMPYASALFNNRAKSDRPKVIPKHSTNLAFTGE
                     FVEQPYQMIFTEQSAVRSGEIAAFHFAGIPMSRLVKTPRYDKDIPTLARAAKKMFE"
     gene            4707..4880
                     /locus_tag="ACG8KR_001541"
     CDS             4707..4880
                     /locus_tag="ACG8KR_001541"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0200909.1"
                     /translation="MQAFEHRLPRGYEKAALHQVIRAAKLKQLLMFLTWLDLLPQMQA
                     FEHRLPRGYEKGV"
     gene            5258..6391
                     /locus_tag="ACG8KR_001542"
     CDS             5258..6391
                     /locus_tag="ACG8KR_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HFQ0200910.1"
                     /translation="MKAVTWQGKQKMEIRNVDDPKILEPTDAIIRITATAICGSDLHL
                     YHHGEAVLEKGYVVGHEPMGIVEEVGSQVQTLKKGQRVVIPFNISCGHCHFCLNHMES
                     QCDESNPDQLYGGLFGFGKLNGNHWGGQAEYLRVPYADSTSFVVPDNDLPDEKVLFLS
                     DILPTAYWSVENAGVKKGDTVVILGSGPVGLFAQKFAVMAGAERVIAVDPVQHRLDKA
                     ANYNGVETYFLEDTAQAGTELYELAKGGADVIIDCVGMDGLEPVKEKAKNLVSLQSGT
                     ISPLQMASQAVKKFGTVQVTGVYMTPASSYPLQEFFMRDIAVKHGQAPIIHLMPKIYA
                     MIAEGLFDPTQIITHSLPLSEAAQAYEIFDKKEDKNIKVVLKP"
     gene            6422..6742
                     /locus_tag="ACG8KR_001543"
     CDS             6422..6742
                     /locus_tag="ACG8KR_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YrhK family protein"
                     /protein_id="HFQ0200911.1"
                     /translation="MPKIKRNTHEIEIGKEEDIEIRGRRFRFYFQNRYTLISLAVDLL
                     TGIFYIFGSLASLTKIPDRYGTYFYLAGAIFLTIRPILRIVRNIFIYDEKKAMKRTSK
                     KKEA"
     gene            complement(6771..7235)
                     /locus_tag="ACG8KR_001544"
     CDS             complement(6771..7235)
                     /locus_tag="ACG8KR_001544"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0200912.1"
                     /translation="MVTYNWIKLREHPELVKQSANWFSSKWGVPAVVYEESIRTGIAN
                     KESIPQWYVVLDEKETIVGGAGVIENDFHNRPDLTPNLCALFVEPEKRKQGIAKRILA
                     EIRKEMHQLGYEKLYLITDHTTFYERYEWTFLTMVKEESGTTARLYQSDTIG"
     gene            complement(7367..8620)
                     /locus_tag="ACG8KR_001545"
     CDS             complement(7367..8620)
                     /locus_tag="ACG8KR_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFQ0200913.1"
                     /translation="MDKFVAFMEKHFIPVASKIGAQRHLVAIRDSFMVSMPLMILGAL
                     AVMINNLPIPGFQELMNSIFGGESWKGFGAAAWNGTFAILSVLIAFLLAYHLANGYRK
                     DGVSAGVISLGSFFALGGALGMSSNGLFIAIIVGIISTEIFVRLSGNEKLIIKMPDGV
                     PPAVAKAFASLLPAMITISAFALVAAIFAAFGVDDIVGSFYTVVQEPFMGLANSYPSA
                     LLLAFITPFLWFFGLHGANMVDPLMQTINAPAIEANVNAIAAGHSAPFIVNKPFFDSF
                     VNLGGTGATLGLLLAIYLVGRKNKPYMVVTNLSIAPGVFNINEPTMFGLPIVLNPIMF
                     IPFILTPMVLVSVAYFATSTGLVPAATVMPPWVTPPIIGGVLATKSIAGGVLAAVNLV
                     LSILIYLPFVKVATIQERRKEALDA"
     gene            complement(8649..8966)
                     /locus_tag="ACG8KR_001546"
     CDS             complement(8649..8966)
                     /locus_tag="ACG8KR_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFQ0200914.1"
                     /translation="MEDQKNLEAIMGLIMFGGNAKSDAMEAIAAAKKGDFTLADEKIK
                     DAEESLVQAHHSQTEMLTQEAQGNHVEVTLLTVHSQDHLMTSIAFTDLAKEIIDLYRR
                     LNA"
     gene            complement(8992..9312)
                     /locus_tag="ACG8KR_001547"
     CDS             complement(8992..9312)
                     /locus_tag="ACG8KR_001547"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFQ0200915.1"
                     /translation="MEKKTIMLVCSAGMSTSLLVTKMQKAAEAKGMEADIFAVSASDA
                     DNQLESKNVDVLLLGPQVRFMQNQFAEKLAPKGIPLDVINMADYGMMNGEKVLDQAEK
                     LINK"
     gene            complement(9776..11191)
                     /locus_tag="ACG8KR_001548"
     CDS             complement(9776..11191)
                     /locus_tag="ACG8KR_001548"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFQ0200916.1"
                     /translation="MKITIPKNFILGAASSAWQTEGWKGKKEGQDSFIDSWYKNEHFV
                     WHNGYGPAVATNFMEQYQQDVDLMKSIHLTHYRTSINWARFFTDYENCIVDEEYAQHI
                     SEMIDSLIEADVEPMICLEHYEVPAFLLEKYDGWSSRKVVDFYAKYAQIAFDRYGDRV
                     KNWFTFNEPIVPQTRCYLDAVRWPHEQNTKKWMMWNYHKVLATAKAVEIYRSGNYGGR
                     IGCILNPEMVYARSNSPEDQKAAYMYDLFYNRVFFDPMVKGYYTEEFLELCQKQSIYF
                     DPATEDLETIQHHTVDFLGINQYYPKRVRAPRYQWNEETPFHPERYYEVFDLPGKKMN
                     NSRGWEIYPKIMYDIAMYLKENYHNIPWLITENGMGREYEEQYMDEKGIVQDDYRIEF
                     IGQHLHYLIKATEEGANCEGYMLWAFTDCVSPMNAFKNRYGLVRIELNDERSRTLKKS
                     ASWYQTLIDKRELDLPDEIYK"
     gene            complement(11208..12524)
                     /locus_tag="ACG8KR_001549"
     CDS             complement(11208..12524)
                     /locus_tag="ACG8KR_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFQ0200917.1"
                     /translation="MRGSGMSFMDKLANVLGNVATRINNLRYIMVIKNAFAALIPVII
                     TGAFGTLFSAMVFDSENGLAQISFLRFLEELKPIASAISYVTLSFLTIYAVFLIGIEL
                     AKLNKVDGVFPGIIAVMSYLSVNPTVYELVNENQTVIVENVLAKQYTDTKGLFLGMLV
                     SILSIELYCWLRKQKKLQLRMPDTVPTNVSASFSALFPTILTVSGIAAAGFIIKELTG
                     MYLYDIIYNVVQRPLEGVVQGLPGILLLMFIAQVFWVIGIHGNQMVKPIREPLLLAAI
                     AVNTEAFEAGKEVPNIITMPFWDMYMSMGGSGVTIGLLIAVLLVGKREDMKQITKLSL
                     APGIFNINEPVIFGMPIMLNPILAIPFILTPLITGTIGYFATALGFAAKAVVMVPWPM
                     PPIVNAYLATAGNIGAVITQLVCIIVSILIYLPFVKVANRVENDGI"
     gene            complement(12674..13372)
                     /locus_tag="ACG8KR_001550"
     CDS             complement(12674..13372)
                     /locus_tag="ACG8KR_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HFQ0200918.1"
                     /translation="MEENKTLVEVTTDKILRYLKEMQLQVGDKLPNEYELSDYLEVGR
                     STVREAVRALASRNILEVRQGAGTFVSDKRGIANDPLGFSLIEDQDRMIRDLFELRYL
                     LEPKMAGLAAEYATKNQIVQMKKLTQKIETSFREGTQEHVQLDIDFHTMIAEASGNVA
                     LLHIIPIINESIALFNKNYDIQHLKIETIDIHTEIVQAIERRNALAAFDAMTVHMAIN
                     RRELQKIIGEKETN"
     gene            complement(13661..14650)
                     /locus_tag="ACG8KR_001551"
     CDS             complement(13661..14650)
                     /locus_tag="ACG8KR_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFQ0200919.1"
                     /translation="MYIADSARYEKMEYRKSGHSGLKLPILSLGLWQNFGDYDPIHNQ
                     REILRGAFDMGITHFDLANNYGGPAGAAEKNFGRIFREDFQAYRDELIISSKAGYHMW
                     EGPYGEWGSRKSIISSCDQSLQRMGLDYVDIFYHHRPDPDTPLEETAEALMQLVRQGK
                     ALYIGISNYNGEDTKKMTEILKRKEAPFIIHQMRYNMFSRALLEDDLSPVLEEEGLGA
                     ITFSPLAQGLLTNRYLHGIPEDSRAHRKEIPFLSEEQVGSTLEKIKALQTIAVSRGQS
                     LAQMALAWNLRQKSVTSVLVGASRLSQLQESVRMMDNLDFSPEEELRIDQILE"
     gene            complement(14730..15563)
                     /gene="msrA"
                     /locus_tag="ACG8KR_001552"
     CDS             complement(14730..15563)
                     /gene="msrA"
                     /locus_tag="ACG8KR_001552"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFQ0200920.1"
                     /translation="MDEEQIFIQTLYNLILNPNTRDWERKVLIQTKNDIRENISVKEQ
                     LSKLEATLRPLAIRMNLTPDVMDFYLLITEGFDKEQKYDFSKHAMQDADYQERAVFAG
                     GCFWCMVEPFESKKGILSVLSGYTGGHVEKPNYDQVSGGYTGHVEAVEIIYDTREISY
                     SELLTIYWQITDPTDTFGQFQDRGKQYRPVIFYQVERQKELAEQSKQKLDSSGTFHQP
                     IVTKIEPAGTFWPAENYHQQFYKKQPKRYKKIQQARNQFLIYQRVKNKWQKNIRKNHF
                     D"
     gene            complement(15738..>16187)
                     /locus_tag="ACG8KR_001553"
     CDS             complement(15738..>16187)
                     /locus_tag="ACG8KR_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0200921.1"
                     /translation="ISQPGHIVVNKETVEAYTTKGMDYFLYDYSPIASLENKEHIIKH
                     EAKIGSQWVNLPYKTTGSSIIYRVTNEKSQVITLPVYAYLGTNVEINHQPVKEQVNAN
                     GLVQISGVKGVADIKVSTSYTVTTIISYLVTLTAYLCLFFKIVFHKR"
BASE COUNT         5032 a         3270 c         2756 g         5129 t
ORIGIN      
        1 tttcccatat tgtgcaaaaa aatacaaggc ggaggctcta ctatgattga agaatatatt
       61 gagaaaaata ttttaagaca gctgttttta tgtgggcaat tctatgtaaa taaagaagtc
      121 aatctagaaa agctctccaa tctcctgcat gtttgcaaaa ccactttact caatgatatc
      181 aataacataa aaaaagaatt tgaagaacag atcgcttata cacatcggga aaaggaccgt
      241 tacactttat atttttcaga acatatacct cgatgcaaga tcatgcagca attgtctcaa
      301 aattcgttgt ttttaaaaac ttgtctgctt tatttggaag aagatgaacc tgattatctt
      361 caattaactg aatgtgaatt catctctgtc agcaaagctt attcattaaa aaaacaagta
      421 ttagcttatt ttaatgactg cgggatcgaa atcgaccgct actcccctcg attcactgaa
      481 atggaaagac gtctgctgct tcttaatgtg tcgtatcgtt taggcggttt taattcttgg
      541 gagctacctg aatcgttttt tgaacgtgcc gatcgtttca ttgaatccgt aacggaaaat
      601 agtggcagat tttatgataa agaaaataaa gaaatcttat cgattggttt tgctatcagt
      661 ttcctacgcc agcaagtgtg tgctgtgaca atagattcaa aatttatcga agaaatcaaa
      721 aaacgtccta tttacaatta tgtcgaatca gcatgggaaa acactgattt ccaaacctat
      781 tataaaaaag aagaattcgc ctttattttg acactattca atctttgtaa ttatggtttc
      841 cattcttatc agttgattgc ggaagacttt caacagctac accaagtgtt tattgacaat
      901 actcccgaaa taaaagaact agttgctacc tttgaatctc acttcaatca agaattattc
      961 ggaaaccaac cgttcgaacg tgcgctgatt catctgatgc gttcggcttg ggataactat
     1021 caattattca tgccagaaaa attttatctt ttaaatgagg aacagacaaa tttatataaa
     1081 gaagtccaga caattttttc cagctggtcc agccagttac cttatgatct acggttgaat
     1141 cctaattgca tgcgggcttt tgtgatcgaa ttgagcggaa ttctccgtct gacaaaagaa
     1201 catctgacta tctatatcgt cacaaattcg gatgttcatt atctgattta tcgggaagcc
     1261 ttggaagctg taactacttt cgattttcaa gttgcaccaa caatttattc aagtatatct
     1321 gatataaaga agtatgcgca gcagtcctca aaccgggtat tatgtgaacg tacattatat
     1381 acaccagatg cggtccaata tgaaaatatt attcctattt cgatcaatac gatcgatcga
     1441 gctatcattt cagctgtgca aaacaaataa ggggctgtga caaaaatcat gtcacagcct
     1501 cttatttgtt gaagatgccc atttctgtaa caatctcttg atacacgtca ggctcacaac
     1561 ctcacttatt ttggacatct acgggtccga tctctttatt taaaaaataa gaaatgatgg
     1621 ttgggataac taccaacgcc gctagttgga aaatatcctg caaggtaggt ttgataatcg
     1681 attcgatgat cccagctgaa acaagaattt ccaaacttag aagtatatag cctcccaaca
     1741 tctttttaat agcattgttc tcgctgactc ggtcgctttg cacttttgta tggacaaata
     1801 aatatttcaa aaaatccttg attgatagac aaacacccca aaccaataaa cagatagcaa
     1861 agatatcaag tcccagtaca agtttttcga aatatggaag aagaaactgc atcaactctg
     1921 tcgtcatcct acctctcctc ttctttgttt ttctttgtct tctatttcct gattcaagaa
     1981 ataagaaatg actgttcgaa tgataataat aagtgcaagc ttcaaaatat ctgtccaagt
     2041 cggcttgata actgactcga tgatatcagc aacgactagt acttccaagc ttaacaaaat
     2101 ataacttgct agcagttttc ttgtatctgc attacgagta ttacgttctg catagttttt
     2161 cttgacatga gaccaatcaa aaagtttgaa tagaacaaga acgatcccga caattaagat
     2221 accgatagaa caaatattta aaatagcaat gatccattct agaaattcaa ttaaatttga
     2281 gcgaaacata tacgctacct actttctttt aatttgttcc tattctttta ttgtaaaaga
     2341 aagttacctt gttcgtctat ttaaaagttg ttctaatgat aacggttcgc ttaaattttt
     2401 ctttttcact ctcccatttt tctccctcca tccataaatc gtggaagtat ccaaaacaac
     2461 gtagcaagtt aaaataaggc ttagtccgtc atcagctttt caaagaagca ctgtctcggt
     2521 gctttttttg tacccaaatt tctaaaaata gaaccataca cctcctcaaa tcttttcttc
     2581 ttacctaccc tatttttatt tacaggaaaa tcagacactt cttctttttt gtcaaaattt
     2641 cctttttctt ctcgtcctat catttggcat tctaataaaa acgaactatc cttggatata
     2701 gaaagagaaa acaaggaggc gtttttcatg agtaaaaaat taatcaaagt cggcatcggt
     2761 ttaggtttgc ttgcccttgg cgctgcatat ctaggaaaaa agacaggact attcgaagat
     2821 gacagtcatt tatatgatga attcgaatca atctagcatc ctatttagtt gaatcactac
     2881 ggacatactt ggaagacatt ggaggaatca caatgagaaa aagtaaagca ataatgattg
     2941 gtgccggtat tgccaatatg gcagcggctg tttatttgat tcaagaaggc aaatggaaag
     3001 gggatcagat caccttttat tcattggatg atcacggttc aaatgatggt gcaccgaccg
     3061 acactgtagt ggatgaatat tggaataaaa accatccatt agaaaaccaa aaaggctata
     3121 ttgctcgagg cggtcgcatg cttaattacc gtacctatgt agatttgatg gatctgttaa
     3181 gtcgaatacc ttctgttact gagccaggga tgactgctga agaagatacg agagattttg
     3241 atgcaaaaca ccgaaccttt gacaaagcac gtttgatgga aggtggcaaa ggaatcattg
     3301 atggcggaaa actgggattc aataataaag accgtattct tctgactaaa ctgattgcta
     3361 tgcctgattc agaagaagaa aaactagaca atatcactat cgaagaatat ttcaaagatg
     3421 atccacactt ttttgaaaca aacttctggt ttatgtggga aacaacattt gctttcagaa
     3481 cacgtagttc tgctcaagaa ttgcgtcgct atatgcatca aatgatttat gaattcactc
     3541 aaatcgaaca tctagtgggc gtcaatcgaa cacgttacaa tcaatacgaa agtattatgc
     3601 ttcctttgat cgagtacttg aaagaacaag gatgcaaaat cattttgaac cgccgtgtca
     3661 ctgactggga attcaaagaa acaccgatgc aagatgaaat cactgtgact ggtttgaaaa
     3721 ttaccaatac actgactcaa gaagaagaat acgttgtggt cgatgaagat acagcagtta
     3781 ttttcactaa tgggtccatt acagattcag ctactttagg agattacgaa acaccagctg
     3841 tagaaaatat ggattatggt gcagcttcta gtctctggaa aaaagccagt gaacgtttct
     3901 acaacttggg aaatcctgac aaattctttg ctgatcgaga tgcaagcgaa tgggtcagct
     3961 ttaccctgac gacgaaaaac catttgttgt tgaatgaaat cacccgtatc acgactcaag
     4021 aaccaggaaa tgccttgaat tcatttattt caaccacacc gatcacgccg ttaggccaaa
     4081 aagatgtcaa tatgtctatt gtcgtgcatc atcaaccgca ttttacgtct cagaaaccaa
     4141 atgaaacagt tatttggggt tatttccttt atccaagacg acgtggcgaa tttgtggata
     4201 aacagtatat taaaatgaat ggaaaagaaa tgctagaaga actgattgga caactttcaa
     4261 aagtagatcc tggaccagtg aatattcgtg aaaaagaaac agaaattttc gacagcgtaa
     4321 taaataatat ccctgtctat atgccttatg cttccgcgtt gttcaataat cgtgccaaaa
     4381 gcgatcgacc aaaagtcatt cctaaacatt cgaccaatct ggcctttaca ggagaatttg
     4441 tcgagcagcc ttatcagatg atctttactg aacaaagtgc tgtacgttct ggagaaatcg
     4501 ctgctttcca ttttgcgggt attcctatgt cccgtcttgt caaaacaccg cgttacgaca
     4561 aagatattcc tactctagct cgtgccgcaa aaaaaatgtt tgaataatca agaggttgtg
     4621 aaaaagctgc cctgcatcaa gtaataagaa caaccaggct aaaacagctt cttatgtttt
     4681 tgatttggtt ggacttatta ccgcagatgc aagcttttga acaccgttta ccaagaggtt
     4741 atgaaaaagc tgccctgcat caagtaataa gagcagccaa gctaaaacag cttcttatgt
     4801 ttttgacttg gttagactta ttaccgcaga tgcaagcttt tgaacaccgt ttaccaagag
     4861 gttatgaaaa aggcgtttag tttcgagtat gaagaaaaat tttgtgaaaa tggttcctca
     4921 cgtttttgca taaatttgac ttaataccga gaaactgatc ccttctgtca taacctcttt
     4981 ttatttatgt ctgtttcttt ttgaaaacaa ctattttttc aacgtttact ctcttgccaa
     5041 tccaacatca tctaacggat acaactattt caatgattac cattacacta aaaaatcgga
     5101 aacacacctc tccgaacaca aatacataag actatagaat gtttcattta agaaaaaaag
     5161 cgtttcatta tcaaaaaggt aagatctcct ttaaaatgaa attatctgta gtactaataa
     5221 tgcagacaat tttatgaatc tgaaaggagc atctccaatg aaagcagtca cttggcaagg
     5281 aaaacagaaa atggaaatca gaaacgtaga tgatccaaaa atattagaac caacagatgc
     5341 aatcatccgt atcacagcaa cagccatttg cggctccgat ctgcatcttt accatcatgg
     5401 tgaagcagtt ttggaaaaag ggtatgttgt aggacacgaa ccaatgggaa tcgttgaaga
     5461 agtgggatct caagttcaaa cattgaaaaa agggcaacgt gtggttatcc cttttaatat
     5521 tagttgtggt cattgtcatt tctgcctgaa tcacatggaa agccaatgtg atgaatcaaa
     5581 tccagatcag ttatatggcg ggcttttcgg atttggcaag ttgaacggta atcattgggg
     5641 tggtcaagcg gaatatctaa gagttcctta tgcagactct acttcatttg ttgtaccaga
     5701 taatgatctt cctgatgaaa aagttttatt tctatcagat attttgccca cagcttattg
     5761 gagcgttgaa aacgcaggag taaaaaaagg cgatacagtt gtgattctag gttctggtcc
     5821 agtcggtcta ttcgctcaaa aattcgctgt tatggctgga gcagagcgcg taatcgcagt
     5881 agatccagta cagcaccgtc tagataaagc ggctaattac aatggggttg aaacttattt
     5941 cttagaagac acggcacaag ctggaacaga attgtatgaa ctggctaaag gtggcgcaga
     6001 tgtcattatc gattgtgtag gaatggacgg attagagcct gtcaaagaaa aagcgaaaaa
     6061 tttagtaagc ttacaaagcg gcacaatctc tccattacag atggcatcgc aagctgtcaa
     6121 aaaatttgga acagtccaag taactggtgt ttatatgact ccagcttctt cttacccgct
     6181 gcaagaattt tttatgcggg atatcgctgt caagcatgga caggcaccaa ttatccatct
     6241 aatgccaaaa atttacgcaa tgattgcaga aggactattc gatcctacac aaatcattac
     6301 tcattcgctg ccactatcag aagcagcaca agcatatgag atctttgaca aaaaagaaga
     6361 taaaaatata aaagttgttt tgaaaccttg aattcagtaa aaaatacagg tgattttctt
     6421 tatgcccaaa atcaaacgaa atacccatga gatcgaaata ggaaaagaag aagatatcga
     6481 aatacgagga cgtcgcttcc gcttctattt ccaaaatcgt tacacattga tctcactggc
     6541 tgtcgattta ttaaccggga ttttctatat ttttggaagc cttgcttcct taactaaaat
     6601 ccccgatcga tatggcacct atttttattt ggctggtgct attttcttga cgattcgtcc
     6661 tattctcaga atcgtaagga acatttttat ttacgacgaa aaaaaagcaa tgaagagaac
     6721 aagcaaaaaa aaagaggcat gagaattttt caaatctcat gcctcttttt tcaccctatc
     6781 gtatcacttt gatacagacg agcggtcgtc ccactttctt ccttgaccat tgtcaaaaat
     6841 gtccactcgt atcgttcata aaacgtggta tgatctgtaa ttaagtaaag tttttcgtac
     6901 ccaagttgat gcatttcttt tctgatttcc gctaatatcc gcttggctat cccctgctta
     6961 cgcttttccg gttcaacaaa cagggcacaa agatttggtg tcagatcagg gcggttatgg
     7021 aagtcgtttt caatgacgcc cgctcctccg acgatggttt ctttttcatc taagaccaca
     7081 taccattgag gaatactttc cttattagcg attccagtac gaatactttc ctcatacaca
     7141 acagcaggaa ctccccattt tgatgaaaac caattcgcgc tttgttttac taattctggg
     7201 tgttctctta atttgatcca gttatatgta accaatgttt aaacctccga ctaaaataaa
     7261 acgtttacaa aaattataca agtctttatg aaaattagca atgacaacaa tagtgatgta
     7321 aaaaaaaaaa cggaaaacaa tcacttgttc tccgtttttt gattaatcaa gcatctaaag
     7381 cttcttttct tctttcttgg atcgtagcta ccttgacgaa cggtagatag atcaaaatag
     7441 aaagaaccaa attgacagcg gctagtacac cacctgcaat actttttgtt gccaatacac
     7501 cgccaataat tggtggagtt acccatggag gcataacggt agctgctggc actaaacctg
     7561 tagaagtcgc aaagtaagca acactaacta agaccattgg tgtcaaaata aatggaatga
     7621 acatgatcgg gttcaacacg ataggtaacc caaacatcgt tggttcatta atattgaaga
     7681 cacctggcgc aatcgacaag tttgttacaa ccatatatgg tttgttttta cgtcctacta
     7741 aataaatagc taacagtaaa cctaaagttg cacctgttcc tcctaagttg acaaagctgt
     7801 caaagaaagg tttgttcacg ataaacggtg cgctatgtcc agctgcgatt gcattaacgt
     7861 ttgcttcaat ggctggcgca ttgattgttt gcatcaaagg atcgaccata ttcgctccgt
     7921 gtaaaccaaa gaaccataag aatggtgtga taaatgcaag cagtaaggca gatggataag
     7981 agtttgcaag tcccataaat ggttcttgga ccactgtata gaaagaacct acgatatcat
     8041 caacaccgaa tgcagcaaaa attgcagcaa ctaaagcaaa cgcagagata gtgatcattg
     8101 ctggtagtaa tgaagcaaat gcttttgcaa ctgccggtgg cacaccatca ggcattttga
     8161 tgatcaattt ttcatttccg cttaaacgta caaagatttc tgttgaaata atcccaacaa
     8221 taatagcaat aaacaaaccg tttgagctca tacctaatgc tccgcctaaa gcgaagaaag
     8281 agcctaaaga aatcacacct gcggaaacac cgtcttttcg gtagccgtta gctaaatgat
     8341 aagcaagcaa gaaagcaatc aagacagaaa gaatggcaaa tgtaccgttc caagcagcag
     8401 ccccgaatcc tttccatgat tcaccaccaa aaattgagtt catcaattct tggaacccgg
     8461 gaatcggtaa gttattgatc attactgcta atgctcctaa gatcattaac ggcatactca
     8521 ccataaatga gtcccgaatc gcaactaaat gccgttgtgc accaattttt gatgctacag
     8581 gtataaaatg tttctccata aatgcaacaa atttatccat aaatcttccc tcttttcttt
     8641 actgtagatt acgcgttcaa tcgacgatac agatcaatga tctcttttgc taaatctgta
     8701 aaagctatag atgtcatcag atgatcttga ctgtggaccg tcaaaagcgt gacttcaacg
     8761 tgatttcctt gtgcttcttg tgtcagcatc tctgtttgag agtgatgtgc ttggactaaa
     8821 gattcctctg catctttgat tttttcatct gctaaagtga aatcaccttt tttcgcagca
     8881 gcaatcgctt ccattgcatc gctttttgca tttccgccaa acatgatcag acccatgatg
     8941 gcttctagat ttttttgatc ttccatttat tgtatcctcc gataattttt attatttgtt
     9001 tattagtttt tctgcttggt cgagtacttt ttcaccattc atcatgccat agtcagccat
     9061 attgattaca tctaaaggaa tgcctttagg tgctagcttt tcagcaaatt gattttgcat
     9121 aaagcgtact tgcggtccta aaagtaaaac gtctacattt ttagattcta gttgattatc
     9181 cgcatctgaa gcagatacag caaagatgtc tgcttccatt ccttttgctt ctgctgcctt
     9241 ttgcattttt gtgactagga gacttgtact cattccagct gaacatacaa gcatgattgt
     9301 tttcttttcc attttaaaca cgttcctttc tatacttaca cttgtatata agcaagtagc
     9361 gtgccaactt taaaagcgct ttaaaaagtt gatatcatca catttaaggt ctataccaag
     9421 ttgcatacac actaaaaaag aaagcgcaca ctaaacacac aaattaattt ttattacctt
     9481 ttttctttta acgtgcacaa aaaaagttgt gacacaaaac ttgtcacaac ttttttcaaa
     9541 taaacggtat tcaaaagctt gcatctgcgg taataagtcc aaccaagtta aaaacataag
     9601 atgttgtttt tactttgcta ttcttattac ttgatgcagg acagcttttt cacaacctct
     9661 caataaacgg tgttcaatca gctgaccctg ggtattagct caaaacgctg gattcacaac
     9721 ctcttttatt tcaaataacc agtaaataca atgaacatct acaaaaaact tctatttatt
     9781 tatagatttc gtcaggcaaa tccaattctc tcttatcaat caaagtctga taccacgaag
     9841 cagatttttt caatgttctg cttcgttcat cgttcaattc gattcgaact aatccatatc
     9901 gattcttaaa tgcattcatt ggtgacacac agtcagtgaa tgcccataac atatagcctt
     9961 cgcaatttgc gccttcttct gttgctttga tcaagtaatg caaatgctgt ccgataaatt
    10021 cgatacggta atcatcttgc acgattcctt tctcatccat atactgttct tcatattctc
    10081 ttcccatacc gttttctgtt atcagccaag ggatattatg ataattttct tttagataca
    10141 tggctatatc atacatgatt ttcggataga tttcccatcc cctggaatta ttcattttct
    10201 tcccaggtaa atcaaagact tcataatatc gttcaggatg gaaaggtgtc tcttcattcc
    10261 attgataacg aggggcgcga acgcgtttag gataatattg attgattcca aggaaatcta
    10321 ctgtatgatg ctgaatcgtt tctaaatcct ctgttgcagg atcgaaataa atcgattgtt
    10381 tttgacacaa ctccaggaac tcttctgtgt agtatccttt taccatcgga tcaaaaaata
    10441 ctcgattata aaacaaatca tacatataag cagctttttg gtcttctgga gaattggatc
    10501 gagcatagac catttcagga tttaaaatac agccaatcct tccaccataa tttccgctgc
    10561 ggtaaatctc tactgcttta gccgttgcta aaactttatg ataattccac atcatccact
    10621 tttttgtgtt ttgttcatgc ggccagcgaa cagcatccaa atagcaacgc gtttggggaa
    10681 cgattggttc gttgaatgta aaccagtttt ttaccctatc accgtaacga tcaaaggcaa
    10741 tttgagcata cttagcataa aaatcaacta cttttcttga agaccagcca tcatattttt
    10801 ccaaaagaaa agctgggact tcataatgtt ccaaacaaat catcggttct acgtctgctt
    10861 cgatcaaaga gtcgatcatt tcacttatat gctgagcata ttcttcatct actatacagt
    10921 tctcataatc agtaaaaaaa cgagcccaat tgatagaagt ccgataatgt gtcagatgga
    10981 tggatttcat cagatccaca tcctgctggt attgttccat aaaattagtt gctacggctg
    11041 ggccataccc attatgccaa acaaaatgtt catttttata ccagctatct ataaacgaat
    11101 cctgtccttc tttttttcct ttccaacctt ccgtttgcca agcagaagat gcagcaccaa
    11161 gaataaaatt ttttggaata gtaattttca tgatttcctc ctgtttttta gatcccatca
    11221 ttttccacac gatttgctac ttttacaaat ggcagataga tcaaaataga aacaataata
    11281 caaacaagtt gcgtaataac tgcaccgata tttcctgctg ttgccagata agcattgacg
    11341 atcggcggca ttggccaagg gaccattaca actgctttcg ccgcaaatcc taaagcggtt
    11401 gcaaaataac caatcgttcc agtgataaga ggcgttaaga taaacggaat agctaaaatt
    11461 ggattcagca tgatcggcat accaaaaata actggttcat tgatgttgaa gatcccagga
    11521 gcaagggata acttggtgat ttgtttcata tcttcccgtt ttccaactaa caatactgca
    11581 atcagtaaac cgatcgtgac tccagacccg cccatactca tatacatatc ccaaaatggc
    11641 atagtaataa tgttcggtac ttcttttcca gcttcaaaag cttccgtatt tacagcaatt
    11701 gccgctaaaa gcaacggttc ccgtatcggc ttcaccattt gatttccatg gataccaatc
    11761 acccaaaaga cttgcgcaat aaacatcaat agcaaaatac ccggtaaccc ttgtacgaca
    11821 ccttctaatg ggcgttgtac gacattgtaa atgatatcat aaaggtacat tcctgtaagt
    11881 tctttgatga taaatcccgc agccgcgata cccgatacag tcagaatcgt cgggaataaa
    11941 gcagaaaaac tagcagaaac attcgttggc acggtatcag gcattctcag ctgtagtttc
    12001 ttctgtttcc tcaaccagca ataaagttca atagaaagga tggagaccaa cattcccaaa
    12061 aataaccctt tcgtatctgt atattgttta gccaatacgt tttctactat aacagtctga
    12121 ttttcattca ctaattcata aacagttgga ttaacagata aataagacat cactgcgatg
    12181 ataccaggga atacaccatc cactttattc agcttggcta attcgatccc tatcagaaaa
    12241 acagcataga tggtcaagaa acttagtgtg acatagctga tggcagatgc gataggcttt
    12301 aattcttcta aaaaacgcaa gaaacttatc tgtgctaagc cattttccga gtcaaagacc
    12361 attgctgaaa acaatgtgcc aaaagcaccc gtaataatta ccggaatcaa tgcagcaaat
    12421 gcgtttttta tcaccatgat ataccttaaa ttattgattc tcgttgccac gttccctaaa
    12481 acattggcta atttatccat aaaactcatt ccagatcccc tcattcctgc ttcttctcct
    12541 tgacgtcatc tctttttgtc tattagtcag cgcttacatt tcgatgaaca gaaactattt
    12601 actcctcttt tttaccaaat ataaaacaaa aattcatctg atgtatttat cataaaatac
    12661 atcagatgaa ctgtcaatta gtttcttttt ctcctattat tttctgtaat tctctccgat
    12721 ttattgccat atggacagtc attgcatcaa acgcagcaag tgcattccgc ctttcgatgg
    12781 cctgaacgat ctctgtatga atatcgatcg tctcgatttt cagatgctgg atatcataat
    12841 ttttattaaa caaagcaatt gattcattaa tgattgggat aatatgcagc aaggctacgt
    12901 ttccgctagc ttctgcaatc atcgtatgaa aatcgatgtc taattgaaca tgttcttgtg
    12961 tgccttctcg aaaagaagtt tcaatctttt gcgttaattt tttcatttgg acgatttgat
    13021 tcttcgttgc atattccgct gccaaacctg ccatcttagg ctccaataaa tatctcagtt
    13081 caaataagtc tcgtatcatc cgatcctgat cttcaatcaa agaaaaaccg agtgggtcat
    13141 tggcaatccc tcgcttatca ctgacaaatg tccccgctcc ttgccgaact tcaaggatat
    13201 tcctggaagc tagagcccgc acagcttctc gcaccgtgct tcgccccacc tctaaataat
    13261 cggacaattc atattcattc ggcagtttat ctcctacttg caactgcatt tcctttaaat
    13321 atctaaggat cttgtcagta gttacttcta ctagggtttt attttcttcc aaaccacata
    13381 cctccttttc atctgatgta ttttttcgta attattataa cacgtttgaa tcatctgatg
    13441 attttttgta tacaaaattg attaatccgt tcatagacat acaaacgaag aagatattta
    13501 ccataaaaaa gactaggata aaattcactc ctagccttcg atcattttta ttttgaacga
    13561 caaaaaatat gatgttcaat aacagacgat cttcattcga ttcttgatgt catcttttgt
    13621 tttttttaat atatggataa aaagtttgat tacatcgttt ctattctaga atttggtcga
    13681 ttctcaattc ttcttctggt gagaaatcta aattatccat cattcgaaca ctttcttgta
    13741 actgacttaa ccgacttgct ccgaccagta cacttgtaac agatttttgt cgcaaattcc
    13801 atgcaagggc catttgcgct aatgattgtc ctcgtgatac ggcgattgtt tgcagtgctt
    13861 tgattttttc cagagttgat ccaacttgtt cttctgaaag gaatggtatt tccttacgat
    13921 gtgctctaga atcttcagga ataccatgta gataacgatt tgtcagcaac ccttgagcaa
    13981 gcggactgaa cgtgattgcc cctagccctt cttcttctaa tactggagat agatcgtctt
    14041 ctagtagtgc acgagaaaac atattgtagc gcatctgatg aataatgaac ggtgcttcct
    14101 ttctttttaa aatttcagtc attttttttg tatcttctcc attatagttg gatataccaa
    14161 tatataatgc ttttccttgt cttacaagtt gcatcaaagc ttctgctgtt tcttccaaag
    14221 gtgtatcagg gtctgggcga tgatgataga agatgtcaac ataatctagc cccattcttt
    14281 ggagactttg gtcacaactt gaaatgatgg atttacgaga gccccattcc ccatatggcc
    14341 cttcccacat atgataacct gctttgctag aaataatcag ttcatcacgg taggcttgaa
    14401 aatcttctct aaaaatacgc ccaaagtttt tctctgcagc tccagcaggg cctccataat
    14461 tattcgcaag gtcgaaatgg gtaattccca tgtcgaatgc gcctcttaaa atctctcttt
    14521 gattatggat aggatcataa tcaccaaaat tttgccataa tccgagagat aaaatcggta
    14581 atttcaaacc tgaatgaccg gatttacgat attccatttt ttcataacgt gcactatctg
    14641 caatatacaa tttttctcct cctcatcaaa tctttttaaa acgctttctt ttttctattt
    14701 ttagtttact acattttaaa gaaaaggagt taatcaaaat gattttttcg tatatttttt
    14761 tgccatttgt tttttactcg ttgatagatc agaaattgat tacgtgcttg ctggattttt
    14821 ttataacgtt tcggttgttt tttgtaaaat tgctgatgat agttttctgc tggccaaaac
    14881 gtaccggctg gttcgatttt tgtgacgatt ggttggtgga aagttcctga actgtctagt
    14941 ttctgttttg attgttccgc taattctttt tgtcgttcga cttgataaaa aatcactgga
    15001 cgatattgct tccctctatc ttgaaattgt ccaaatgtat ctgtgggatc agtaatttgc
    15061 caatagattg tcagcaactc gctataactt atttctcttg tgtcataaat gatctccaca
    15121 gcttccacat gacctgtgta accaccactt acttgatcat aatttggttt ttcaacatgt
    15181 ccgcccgtgt atcctgacaa aacagatagg atcccttttt tcgattcaaa cggctcgacc
    15241 atacaccaga aacatccccc tgcaaatact gcacgttctt gatagtcagc atcttgcatg
    15301 gcatgcttgg aaaagtcata tttttgctcc ttatcaaatc cttccgtaat caataaataa
    15361 aagtccatta catcaggcgt cagattcatt cggatagcta atggacgtag tgttgcttct
    15421 agttttgata actgttcttt aacggatata ttttctctta tgtcattctt tgtctgaatc
    15481 aatacttttc gttcccaatc tcgtgtattc ggattcaaaa tcagattata aagtgtttga
    15541 atgaatattt gttcctcatc catagattct cctcctttcc gaagaagaca agctagtgac
    15601 tttagctgta atttatcata aagtgaggaa ataagcgatt acttcatccc cctacagatt
    15661 tttcgtataa aaaagagaga atccttatcg acagattctc ctttttaatc aatttatttt
    15721 ataaatgaaa agttaattta ccttttatgg aaaactattt taaaaaataa acataaatat
    15781 gcagttaatg taacaagata agaaataata gtcgttactg tataacttgt gcttacttta
    15841 atatcagcaa ctcctttgac tccgcttatt tgtaccaatc cattagcatt cacctgctcc
    15901 tttacaggtt gatgatttat ttctacattc gttcctaaat aagcatatac aggaagtgtg
    15961 attacttggc tcttctcatt agttactcta tatatgatag aactaccagt tgttttataa
    16021 ggaaggttta cccattgact gccaatcttt gcttcgtgtt ttattatatg ctccttattc
    16081 tccaatgaag cgataggaga ataatcatat aaaaaataat ccattccttt agtggtatac
    16141 gcctctactg tttctttgtt tacaacaata tgtcccggct gagaaat
//
LOCUS       DAZKLD010000060        16104 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.060, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000060 DAZKLD010000000
VERSION     DAZKLD010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16104)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16104)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16104
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.060"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..361
                     /locus_tag="ACG8KR_001554"
     CDS             <1..361
                     /locus_tag="ACG8KR_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HFQ0200922.1"
                     /translation="SDSSTTTSDSTDTSASSDSTDTSSTTSDSSDSSTSSDSTDTSST
                     TSDTRHASVSFSKTSSDNRTNFNLTNKTSNDNGNKSKRALPKTGSQSNNWITLAGVIL
                     LVIGLRITFMSYKKSKR"
     gene            complement(516..1502)
                     /locus_tag="ACG8KR_001555"
     CDS             complement(516..1502)
                     /locus_tag="ACG8KR_001555"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HFQ0200923.1"
                     /translation="MKLHPMWENYPALSKELTETLKRMEETVRLKNKPVEQAIKETIH
                     AGGKLLRPAYQLLFSQFGPEQDRDKAIALAASIEMLHTATLIHDDIVDEADLRRGQPN
                     IRSQFGNVVSVYAGDYLFVCCFKLLSDYSTSLKSLQLNSRSMEKVLNGELGQMDDRYN
                     YELSVKEYLENISGKTAELFQLSCSVGAFESGTSERFAKKAGDIGLSIGMAFQIIDDI
                     LDYTKESQEIGKPVLEDMRQGVYSLPLIYSLQKNKPKLLPYLEKKAAMTEEDVDAVRK
                     IVEHTGGVEEARKLAASYTEKALKEIKKLPATSLRTKENLFSLTQLILDRKD"
     gene            complement(1525..2091)
                     /locus_tag="ACG8KR_001556"
     CDS             complement(1525..2091)
                     /locus_tag="ACG8KR_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HFQ0200924.1"
                     /translation="MSKLQKMIYISLLVAQGVIIGLLENMIPYPFSFAPGAKLGLANL
                     ITIVAIFTMKKRDSFLLLWLRLFLTTLLGGTISTFLYSMSGALLSYFGMLLVKQLGPK
                     RVSIIGISAAGGFMHNVGQLLMASFIAQSWTVMLYLPVLSVLGILSGIAIGIAANYLL
                     RHVQTLQRFQKDYEQGTKQKWSPMFTKK"
     gene            complement(2105..2521)
                     /locus_tag="ACG8KR_001557"
     CDS             complement(2105..2521)
                     /locus_tag="ACG8KR_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HFQ0200925.1"
                     /translation="MSLKEFAEKSLLKPWDVFIILLLTVLSFLPVMIFGLQQNTATAE
                     KEAVLRVDGTEIKTFPLMAGEESNTYTYKDEHGDYNLIEIDGDRVRIKEADCDDQICV
                     RRGWATKNGETIVCLPHKLVIEVRSTDGGDEDSLIY"
     gene            complement(2978..4912)
                     /locus_tag="ACG8KR_001558"
     CDS             complement(2978..4912)
                     /locus_tag="ACG8KR_001558"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFQ0200926.1"
                     /translation="MAKQNIVVVGAGYAGVSATKYLAKKLKKEDVAITLIDRHSYHTM
                     MTELHEVAGGRVEPEAIQYDLQHLFARQKNVQLVTDTVIGIDKERKIVKTKLGSYPFD
                     QLILGMGGEPNDFGTPGVKAHGFTLWSFEDSVRIREHIEKTVAKAALEPDDELRKAML
                     TFVVCGSGFTGIEMIGELIDWKNRLAKDYKLDPNEITLMVVEAMPTILNMLDRNDAAK
                     AERYLRKHNVELLLNAPIVEVAADHIKLKDGRNIPTHTLIWTAGVKGTSDAADFGLES
                     ARGQRLIANEYMQAKGYEDKNIYIIGDLVFYEEFPGTPTPQIVQAAEQTGHTAAANIV
                     ASIKGGEKHKFKGNYQGFMVSIGAKWGVANLFDKIHLSGFLAIVMKHIVNLKYFFDIR
                     SGYYMFQYLMHEFFHIKDDRNVTRGHSSRYGNVLWSVPLRIFYGLVWFVEASKKIIGN
                     GEVFKPSTWFGEGSWFTGNSVFPWTWEYNVTDVASGASAAGDATTAASGAGASAGEAA
                     TQAAHFGLSYAYGEEPMAVIEKSPDWFTKIMQVIMPNNDVAMFFQKFMVFFEIALALA
                     LIAGLFTWLCSATTIVLVITFCLSGMFYWVNIWFIFVAFALMNGSGRAIGLDRWVIPW
                     LQRTLGNWWYGRPSSQYGKK"
     gene            5214..6149
                     /gene="pplA"
                     /locus_tag="ACG8KR_001559"
     CDS             5214..6149
                     /gene="pplA"
                     /locus_tag="ACG8KR_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular electron transfer flavoprotein
                     PplA"
                     /protein_id="HFQ0200927.1"
                     /translation="MKKKRFVTGFAVLAFSALVLGACGADDNGSSNSSSESSTAQSST
                     AKSTTESSAKVVAGGDLQDGTYKLEEKNYSNGYRAVFEMVVKDGKITESKYDNVNENG
                     ESKTKDAEYNKNMEAKSGTNPEKYIPELNDQFLKAQSASGVEVVTGATHSSESFQNYA
                     QQLIQAAQAGNTDTIEIDNGADLKDGTYKLEEKNYSNGYRVQFEMTVAGGKVTESNFD
                     YIDKDGKSKQDDTEYNENMKAKSGTEPKTYIPTLNDEFVKAMGEEDGSPANVEVVTGA
                     THSSHSFIMYAQQLVNAAEKGDTQTIEVDNIVTEK"
     gene            6239..7342
                     /locus_tag="ACG8KR_001560"
     CDS             6239..7342
                     /locus_tag="ACG8KR_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HFQ0200928.1"
                     /translation="MRKKLLFSLITLGISFMVLTGCSNSANKTTTESSAATKILKDPY
                     SDEQFLLGTYVRIRVYDEGKESALKPAFDRVKELGDKITINQKGSEIDEVNEQAGIKP
                     VKVSDDVYTLVKRAYEYSQDSQGGFDMAIGAITQLWRIGFDDARKPSQEEIDQALKLV
                     DYHKIELNDKEKTVYLKEKGMIIDLGAIAKGYITDEVVKVLKKQGVTTAIVDLGGNVY
                     VLGHSPRGENQDWTVGIQDPNMARGSVLGSIKERNKTLVTSGIYERYLEVDGKKYHHL
                     FDPKTGYPFDNDIASVTIITDKSIDGDGLSTAVFSMGVKKGLEYVESELNNGTEAIFV
                     TKDDKVYVTDPIKDTFKLSEDSHYTMGDRSELK"
     gene            7355..8308
                     /gene="menA"
                     /locus_tag="ACG8KR_001561"
     CDS             7355..8308
                     /gene="menA"
                     /locus_tag="ACG8KR_001561"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HFQ0200929.1"
                     /translation="MTLKIFLEVVEIRTKVASVFPFAVGVLFSIAFFHEIHWLNTFLF
                     FIGMLVFDLATTAINNYMDYEKAHSEVYKYEENVIGRRNLSPVLIRNMILAMIAFVFV
                     IGVILTINTGWLLLLMGFICCFIGVFYTFGPIPLSRMPLGEIFSGVTMGLGIFAMVVY
                     VNTFGSDVYDLTVNFSSGQFALVGNLWAIAAIVLASLPLVFTIANIMLANNLRDLDRD
                     IENHRYTLVFYIGRPAGTVLFQLLMYACYFVILMGLLSGIYEWPILVTFLTLPIVKKH
                     LDQFKQTLPQPISFSYSIKNMVLFNASYVLGLLLTIIMQAI"
     gene            complement(9122..10468)
                     /gene="gor"
                     /locus_tag="ACG8KR_001562"
     CDS             complement(9122..10468)
                     /gene="gor"
                     /locus_tag="ACG8KR_001562"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HFQ0200930.1"
                     /translation="MQIYDYIVIGGGSGGIASANRAGMHGAKVLLIEANELGGTCVNV
                     GCVPKKVMWQASTILETIERDASSYGIQADLKNVDFAGLVERREKYIDFLHGAYQRGL
                     DSNHVEAIKGYATFIDSQTIEVNGETYRAPKILIATGGRASKMTIPGGEYAIDSNGFF
                     ELTELPKRAIVLGAGYIAAELSGVFRGLGSEVMWAYRKERPLRTFDKMLSDNLIQMYQ
                     EAGIKTYAHHIAKEITKNNDEYTVLFENGETLTGDCVLFAGGRVPNTEKLGLENTNVE
                     LDKKGFIKVDKYQNTTDEHIFAVGDVIGKLDLTPVAIAAGRRLSERLFNGKKDSYLDY
                     KLVPTVVFTHPPIATIGLTEEEALEKYGENELKVYRSRFTPMYFALNDYRQKCEMKLI
                     CVGKEERIVGLHAIGVGVDEMLQGFAVAIKMGATKEDFDNTVAIHPTGAEEFVTMR"
     gene            complement(10602..11855)
                     /locus_tag="ACG8KR_001563"
     CDS             complement(10602..11855)
                     /locus_tag="ACG8KR_001563"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1901576 - obsolete organic substance
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HFQ0200931.1"
                     /translation="MMKEMTVKRKLKRPEILAPAGTLEKLKTAIHYGADAVYIGGNAY
                     GLRSRAGNFTYEEMAEGVAFAKEHDAKVYVAANMVTHEGNEDGAGEFFRSLRDVGISA
                     VIVSDPALIEICATEAPGLPIHLSTQASATNFETLEFWKEEGLERVVLAREVSMEEVA
                     EIRKNTDTEIEAFIHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYGLFDMPFG
                     QERNSLTDTGEVEEEFSMSAVDMSMIEHIPELIENGVDSFKIEGRMKSIHYVSTVSNV
                     YKAAVDSYMEDPENYVCKQEWIDELWKVAQRELATGFYYNTPTENEQLFGERRKIPVY
                     KFVGEVLSYDEATQIATIRQRNLFSVGDEIEFYGPGFSHFSQTVEVMHNEEGESIDRA
                     PNPMMILTMPVAQPVKPGDMIRKRK"
     gene            complement(11861..12784)
                     /locus_tag="ACG8KR_001564"
     CDS             complement(11861..12784)
                     /locus_tag="ACG8KR_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HFQ0200932.1"
                     /translation="MIELITTVETIEQAEQLLAAGADTLYFGEEQFGLRLPASFTREE
                     QRQLVELAHKYGKKVNIAVNGIIHPEKMKNVPEYLAFLKEINVDVITVGDTGIIYTMK
                     KNPELRIPYIYDAETLVTSARQINFWAKKGAVGAVLAREVPFEEMKAMEEKLDIPVET
                     LVYGATCIHQSKRPLLQNYYNYTKQDEQKDRERGLFISEPKKEETHYSIYEDSHGTHI
                     FASNDLNLSNELNELYQHHYCTWKLDGLFTPGEKFVNITRLFAEAKKQILEGSWDQEK
                     AEKMTSEISKLHPENRELDTGFFYIDPSAIK"
     gene            complement(13005..15497)
                     /locus_tag="ACG8KR_001565"
     CDS             complement(13005..15497)
                     /locus_tag="ACG8KR_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HFQ0200933.1"
                     /translation="MEELFTERAQAVLEIAQEEAKRLKHQSVSSEHVLLALVVEQNGI
                     AGKVLREMDLNETEIYEEIEHLIGYGGIRSYPKGVFLPYSPRMKQVFALASSEVKKTG
                     SQKVGTEHILMSLLKDESIMATRIMINLGISLSKARQVLKQKMGLAGDSAGKGMNRRR
                     NVNVQDKRQTPQGTPTLDSLARDLTKLAKENKLDPVVGRSREVKRLIQILSRRTKNNP
                     VLVGEPGVGKTAIAEGLAQKIILGEVPEDMQEKRLMMLDMGSLVAGTKYRGEFEDRMK
                     KVIDEIYNDGQVILFIDELHTLIGAGGAEGAIDASNILKPALARGELQTIGATTLDEY
                     QKYIEKDSALERRFARIQVDEPTPEEAEVILQGLRTRYEEHHGVEITDEAVRAAVNLS
                     VRYITSRQLPDKAIDLIDESAAKVRLDQTDHLTKSTVIKLEIDELVQEKEAAIQKQDF
                     ENAAQLRRQEKALRKKLQKVSAIEAKQEQGYSDRVTEEDVATVVSEWTGVPLQQLEKK
                     ESERLLELEGLLHERVVGQDEAVKAVSRAIRRARSGLKDPDRPIGSFMFLGPTGVGKT
                     ELAKALSEVMFGSEDALIRVDMSEFMEKYSTSRLIGSPPGYVGYDEGGQLTEKIRQKP
                     YSVILLDEVEKAHPDVFNLLLQVLDDGHLTDSKGRKVDFRNTIMIMTSNIGATQIREE
                     KNVGFNVQDVTKDHKAMQKRILEELKKAFRPEFLNRIDETVVFHSLSKDEIHTIVQIM
                     SKAIIKRLKEQDIQVKITPSAIDVIGKAGFDPEYGARPIRRALQKEIEDRLSEALLGG
                     EIRLGDHVTVGASKGKITLNVRGPKKETVGSL"
     gene            complement(15504..15971)
                     /locus_tag="ACG8KR_001566"
     CDS             complement(15504..15971)
                     /locus_tag="ACG8KR_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HFQ0200934.1"
                     /translation="MAHQNTSDLIESYIKKILENSEMIEIRRIEMADLFNCVPSQINY
                     VINTRFTIQRGYLVESKRGGGGYIRIAKVRISDKKQLLEQINQLFDDTISEKNAFAII
                     QKLYEDQIITKKEGNLMLSAIAKNTLNFNEYEDHTRARILRAFLERLSYEDGK"
BASE COUNT         4726 a         3362 c         2862 g         5154 t
ORIGIN      
        1 ctcagattca tctaccacta caagtgatag cacggacacg tcagcttcaa gtgacagtac
       61 agatacatcc tctaccacaa gtgatagctc agattcatca acttcaagcg atagtacaga
      121 cacgtcgtct accacaagcg atacacgtca tgcaagtgtc tccttctcaa aaacaagttc
      181 ggataacagg acgaacttta acttaactaa taaaacaagc aatgacaatg gaaacaaatc
      241 aaaaagagca cttcctaaaa ctggaagtca atcgaataat tggataacac tcgcaggcgt
      301 tatattactt gtaatcggtt taagaatcac ttttatgagt tataagaaat cgaaaaggta
      361 aaaaaatatt caagataaaa aagagcaacg tcgctaacgg acgttgctct ttatttttgc
      421 tttttatcat aaatacaaga actacaattc aatttgtcaa aaaacacgta gatttccttc
      481 tgagaaattt atttacgtat cgaacctttc cctattcagt ctttgcgatc caaaatcagt
      541 tgggtaaggg aaaagagatt ctcttttgtt cgaagagagg tggcaggtaa tttctttatt
      601 tccttcaaag ccttttctgt gtaagaggcc gctaacttcc tagcttcttc cacgcctcct
      661 gtatgttcaa cgattttgcg tacggcgtcg acatcttctt ctgtcattgc cgctttcttc
      721 tctaaataag gtagtagctt tggtttgttt ttttgcaaag agtagatcaa cggtaaagaa
      781 tagacccctt gacgcatgtc ttctagtaca ggtttgccga tttcctggct ctcttttgta
      841 taatctaaaa tatcatcgat aatctgaaag gccatcccga tagaaagtcc gatatctcct
      901 gctttttttg cgaagcgctc gcttgttcca ctttcaaaag cgccaacaga acaacttaat
      961 tggaacagtt cagctgtctt acctgaaata ttttccaaat attcttttac agaaagttca
     1021 taattatagc gatcatccat ctgccctaat tcgccattca atactttttc catactgcga
     1081 gaattcaatt ggagactttt caaggaagtg gagtaatcag acaatagttt aaaacagcag
     1141 acaaataaat agtctcctgc atagacagaa acgacattac caaactgact acggatatta
     1201 ggctgaccac gtcgcagatc cgcttcatca acgatatcat catggatcag tgtggccgta
     1261 tggagcattt cgatagaagc tgccaaagcg atggctttgt ctcgatcttg ctctggtcca
     1321 aattgtgaga acaatagctg ataggcagga cgcaacagct ttccgccggc atggatcgtt
     1381 tctttgattg cttgttcgac aggtttgttt ttcaggcgga cagtttcttc catccgtttt
     1441 agtgtttctg tcaattcttt acttaaagca ggatagtttt cccacattgg atgtagcttc
     1501 ataaaaaaac tccttgtgtg acgattattt tttggtaaac attgggctcc atttttgttt
     1561 ggttccctgc tcataatctt tttggaatcg ttgaagtgtc tgcacatgtc gtaaaagata
     1621 attcgctgcg atcccgattg caatccctga taaaatccct aaaactgaaa ggactggcaa
     1681 gtacaacatg actgtccatg attgtgcaat gaaagaagcc atcaaaagct gtccgacatt
     1741 atgcatgaaa ccgccggccg cactaattcc gatgatactg acacgttttg gtcctaattg
     1801 cttgaccaat aacattccaa aataactaag caaagcacca ctcatactat aaaggaatgt
     1861 ggaaatcgtt ccgcctagca aagtagtcag gaatagtcgt agccaaagta gtaagaagct
     1921 gtctcgtttt ttcatggtaa aaatggcaac gatcgtgatt agattggcta agcctagttt
     1981 tgcaccaggc gcaaaagaaa aaggataagg gatcatattt tccaataaac caatgatcac
     2041 tccttgtgca acaagtagcg aaatataaat cattttttgt aatttactca tggtgtgtga
     2101 aggtctaata aatcagactg tcttcgtcac ctccatctgt tgaacggact tcgatcacta
     2161 atttatgtgg caaacagacg atggtttcgc catttttcgt tgcccagcct cgacgtacgc
     2221 agatctggtc atcgcaatct gcttctttta tccgtactcg atcgccatct atctcgatca
     2281 agttataatc tccgtgttca tctttgtaag tatatgtgtt gctctcttct ccagccatca
     2341 aagggaatgt tttgatttct gtgccatcca cacgcagtac cgcttctttt tctgctgttg
     2401 cggtgttttg ctgaagacca aaaatcatga ctggtaaaaa agaaagaaca gtcaaaagca
     2461 aaataataaa aacatcccaa ggtttgagca gacttttttc agcaaattct ttaaggctca
     2521 tttttgttct cctttttcca gattaactca cccaatttta gcataaaaac aagaggttgt
     2581 gacacctgtg tcttgctttg agtaataaga acgaagaata gaaaacagct ttccatgttt
     2641 tctaattctt tgggcttatt accgagaagc agcaggtgga acaccgttta taacaagagg
     2701 ttgtgacaga agtgggcaac ttcaagaaat aagaaggaat ttacgaaaat tacttttcaa
     2761 gtttttgtga aatttcggct tatttttcga agaagctact tctggaacac cgtttataac
     2821 aagaggttgt aacagaggtt gtgacacctg tgtcttgctc atggaggctt atctcgggca
     2881 tcttattcct ttaattttac ataaaaagga ggctgtgacg gtttgtctca gcccctttct
     2941 attgaaatct tgcattcaaa taacgttctt tatctattta tttcttgccg tactgggaac
     3001 ttggtctacc gtaccaccaa ttacctagtg tacgttgtag ccatggaatt acccagcgat
     3061 ccaacccaat cgcacgtccg gaaccgttca tcaatgcgaa agcaacaaaa atgaaccaaa
     3121 tgtttaccca gtaaaacatg cctgataagc agaacgtgat caccaataca atcgttgtcg
     3181 cactgcatag ccatgtgaat aatccagcaa tcaatgctaa tgccaaggca atttcaaaga
     3241 ataccatgaa tttttggaag aacattgcta catcattgtt tggcatgatc acttgcatga
     3301 tctttgtgaa ccagtctggt gacttctcaa tcacagccat tggttcttca ccgtatgcat
     3361 aactcaaacc aaaatgtgca gcttgagtcg ctgcttctcc ggcacttgcc cctgctccgc
     3421 tggcagctgt ggttgcatca ccagcagcag aagcaccact ggcaacatct gttacattgt
     3481 attcccatgt ccaagggaac acactgtttc cagtgaacca tgatccttca ccaaaccaag
     3541 tacttggttt gaacacttca ccattcccaa tgattttttt ggaagcttca acgaaccaaa
     3601 ctaaaccata gaaaatacga agtggcacac tccataagac attgccgtaa cgagaagaat
     3661 gtcctcgagt cacgttgcga tcatctttga tatggaaaaa ctcatgcatt aaatattgga
     3721 acatataata accagaacga atatcaaaga agtatttcaa attcacgata tgtttcatta
     3781 cgatagctaa aaatccgctt aaatggatct tatcaaatag gttagctacc ccccatttgg
     3841 caccaataga aaccataaat ccttgatagt ttcctttaaa tttatgcttt tctccgcctt
     3901 tgatcgaagc cacgatattc gctgcggctg tgtgtcctgt ttgttcagca gcttgaacga
     3961 tctgcggtgt cggtgttcct gggaattctt cataaaagac taaatcacca atgatataga
     4021 tgtttttatc ttcgtagcct tttgcctgca tatattcatt ggcgatcaac cgctgtccgc
     4081 gtgctgattc taatccaaag tcagcagcgt cagaagttcc tttgacacca gctgtccaaa
     4141 tcagtgtgtg agtcggaatg ttgcgtccgt ctttcaattt gatatgatca gcagccactt
     4201 ctacgattgg tgcattcaat aataattcca cgttgtgctt acgcaaatag cgttcagctt
     4261 ttgcagcatc atttcgatcc aacatattca agatcgtcgg cattgcctct acgaccatca
     4321 gtgtgatttc gtttggatct aatttataat ctttggctag gcgatttttc cagtcgatca
     4381 gttccccaat catttcaata ccggtaaatc cagaaccaca gacaacgaaa gtcagcatgg
     4441 ctttacgcag ttcatcatct ggttccaatg cagcttttgc tactgtcttc tcaatatgtt
     4501 cccgaatcct tacagaatct tcaaatgacc aaagggtgaa tccgtgtgct ttgacaccag
     4561 gtgtaccaaa gtcattcggc tctccgccca ttccaaggat cagctgatca aaaggatagg
     4621 aacctaattt ggtcttgacg atttttcgtt ctttatcgat accgattaca gtgtctgtga
     4681 caagttgtac attcttttga cgagcaaaca aatgctgcag atcgtattgg attgcttctg
     4741 gttctacacg tccgccagct acttcatgca actcggtcat catagtatgg tacgaatgac
     4801 gatcgatcag agtaattgct acatcttctt ttttcagttt ctttgccaag tattttgtag
     4861 ctgaaacacc ggcatatcct gctcctacaa cgacaatatt ttgctttgcc attgatcatc
     4921 cgctccttat agactatcta tataatgaat atatgataat tcttttagtt tgatacttat
     4981 tacacaatct ccatatatta tatagatgtt ctgttctttt gtctaactcc ttttggattt
     5041 ttttcaaatt ttaaaataaa gcgttttttc acgaaatttg ctgaaagaaa acgtttgaaa
     5101 tattacatgg gttatttatc aaaaatagtt gacttatagg ctaaaaagat tagaatgtat
     5161 aatgaatcac aaagaaaaat tgtggactag aaatggaatg gtggggtatt aaaatgaaga
     5221 aaaaacgttt tgtaactgga tttgccgtat tagcattttc agctctagtg ttaggcgctt
     5281 gcggcgcaga tgacaacgga agctcaaact cttcttctga atcatctaca gcacaatctt
     5341 cgactgcaaa aagcacaaca gaatcatctg caaaagttgt agctggcgga gatttgcaag
     5401 acggcacata taaattagaa gaaaaaaatt attcaaatgg ttaccgtgca gtctttgaaa
     5461 tggttgtaaa agacggcaaa atcactgaat caaaatatga taacgtcaat gaaaacggcg
     5521 aatcaaaaac aaaagatgca gaatacaaca aaaacatgga agcaaaatct ggcacaaacc
     5581 cagaaaaata cattcctgag ttgaatgacc aattcttaaa agcacaaagc gcaagcggtg
     5641 tggaagtcgt aactggagct actcactcat ctgaaagctt ccaaaactac gcacaacaat
     5701 tgatccaagc tgctcaagct ggtaacacag atacaatcga aatcgacaat ggtgctgatt
     5761 tgaaagacgg cacttacaag cttgaagaaa agaactactc taacggctac cgtgtgcaat
     5821 tcgaaatgac agttgctggc ggcaaagtaa ctgaatcaaa ctttgattat atcgacaaag
     5881 acggtaaatc aaaacaagat gatacagaat ataatgaaaa tatgaaagca aaaagcggta
     5941 cagaaccaaa aacttacatc cctactctga acgacgaatt cgtcaaagca atgggcgaag
     6001 aagatggctc acctgctaat gtagaagttg ttacaggtgc tacacacagc tcacattcat
     6061 tcatcatgta cgcacaacaa ttagtgaatg cagctgaaaa aggcgacact caaacaatcg
     6121 aagtagataa tatcgtaaca gaaaaataat cacataatca tactaataaa agttggaggg
     6181 tgggacgata gccagattag gcagtcgtct cacctttcaa aatgaagaga ggaaaaagat
     6241 gcggaaaaaa ctactatttt ccttaatcac actgggcatc tcttttatgg tgctaactgg
     6301 ctgcagcaac tcagcaaata aaacaaccac tgaaagttca gcagctacca agatattgaa
     6361 agatccatat tctgatgaac agttcttgtt agggacttac gttcgtatcc gtgtgtatga
     6421 tgaaggcaaa gaatcagcat tgaaacctgc cttcgatcga gtaaaagaac ttggtgataa
     6481 aatcacgatc aatcaaaaag gttctgagat cgatgaagtg aatgagcaag ctgggatcaa
     6541 accagtaaaa gtttctgatg acgtctacac gttagtcaag cgggcctacg aatatagcca
     6601 agattctcaa ggcggattcg atatggcgat tggtgcaatc acacaattat ggcgaatcgg
     6661 ttttgatgac gccagaaaac cttcccaaga agaaatcgat caggcattga aattagtgga
     6721 ttatcataag atcgaactaa atgacaaaga aaaaactgtt tatctaaaag aaaaaggaat
     6781 gatcatcgat ttaggtgcaa tcgccaaagg ttacattacg gacgaagtag tgaaagtctt
     6841 gaaaaaacaa ggagtgacca ctgccatcgt tgacttgggc ggtaatgtct atgtcttagg
     6901 ccacagccca agaggcgaaa accaagactg gacagtcggc atccaagatc cgaacatggc
     6961 tcgcggatct gttttaggtt ctattaaaga acggaacaaa acattagtta cctctggaat
     7021 ttacgagcgt tatttagaag tagatggcaa aaaataccat catttgtttg atccaaaaac
     7081 gggttatccg ttcgacaatg atatcgcaag tgtcacgatc ataacagata aatcaattga
     7141 tggtgacggg ttgtctacag cagtcttttc aatgggtgtg aaaaaaggac tggaatacgt
     7201 cgaaagcgaa ttgaataacg ggacggaagc gatcttcgtg acaaaagacg ataaagtata
     7261 tgtgaccgat ccaatcaaag acaccttcaa attatcggag gactcccatt acacgatggg
     7321 tgaccgttct gaactaaaat aaggagtacg aaccatgaca ctaaaaatct ttttagaagt
     7381 agttgaaata cgaacaaagg tggctagtgt atttcctttt gcggtagggg tattattctc
     7441 tatcgctttt tttcatgaga tacattggct aaacactttt ttatttttta ttggaatgct
     7501 agtttttgat ttagcgacta ccgctatcaa taattacatg gattatgaaa aggctcattc
     7561 tgaagtatat aagtatgaag aaaatgtcat tggccgcaga aatctatctc ctgtattgat
     7621 tcgaaacatg attttagcta tgattgcatt tgtttttgtt atcggcgtta ttctaacgat
     7681 caatacgggt tggcttttac ttttgatggg atttatctgt tgttttattg gagtattcta
     7741 tacattcgga ccgattcctt tatctcgaat gcctttaggg gaaattttca gtggggtgac
     7801 tatgggcctt ggaatttttg ccatggtcgt ttatgtcaat acattcggtt cagatgtcta
     7861 tgatctgaca gtcaactttt catctggaca atttgctttg gtaggaaatc tttgggctat
     7921 agcagcaatc gtattagctt ccttaccctt ggtgtttacg attgccaata tcatgttggc
     7981 taataatttg cgggatcttg accgtgatat tgaaaatcat cgctatacac tagtttttta
     8041 tatcggacgt cctgcgggaa cagtcttatt ccagctattg atgtatgctt gctatttcgt
     8101 gattttaatg gggttgttga gcggtatcta tgaatggccg atcttggtca ccttcttgac
     8161 cttgccaatt gtgaagaagc atttggatca attcaagcaa acgttacccc agcctatcag
     8221 tttcagctat tccatcaaaa atatggtctt attcaatgcc agttatgtat tggggctatt
     8281 actaacaatc attatgcaag ccatttaaca gcatgaagag gttgtgaaaa aactgccctg
     8341 catcaagtaa taagaacagc caaataaaaa tagctcctta tatttttcgt ttggttgggc
     8401 ttattaccgc agatgcaagc ttttgaacac cgtttatcag gaggttgtga aaaagtcgct
     8461 tagtttcgag tattaagaaa aattttgtga aaatggcttt ccacattttt gcataaattt
     8521 ggcttaatac cgagaaactg gcttttgaac accgtttatc agaggttgta aaatcagtgt
     8581 tttgtcctga gtattagaga aacaaatgga aaaatagttc ctcatatttt gccgtttttt
     8641 gagctaatac cgaaggacag ctgattgaac actatttgtt cggaaaaagt gactaactat
     8701 gacacaattt ttttcacagt cccttttttc acttgatttg attcgtacca gcgaacttga
     8761 cgagaacttt cttggtttgg taactatatg gtattctttt aacatcctcg tttgcttctt
     8821 ctaatttatt tatacaaagt cacacttttt taaacatctg ttcttcgata catttctatt
     8881 tcttttaaac atcgatacca atattttgaa cttaagagtg cataccaaat ggattcattt
     8941 actcttcgtc agttacgaag ataatcaaaa tctccaactt ccctacttat ttcgttttga
     9001 gaattatcga agcaatctta cataaataac taaaaaacaa aaaaccagaa aatcagtata
     9061 ctgattttct ggttttacat ggtttgttca ataaaaagct gttggaactg ttaatattaa
     9121 attatcgcat cgttacgaat tcttccgctc ctgttggatg gatggctact gtattgtcaa
     9181 agtcttcttt agttgcaccc atcttgatag ccacggcaaa accttgcagc atctcatcga
     9241 caccaacacc aatagcatgt aagccgacga ttctttcttc ttttccgaca cagatcaatt
     9301 tcatttcgca tttttggcga taatcattta atgcaaagta cattggggta aagcgtgaac
     9361 gataaacttt caattcattt tcgccatatt tttccaatgc ttcttcttct gtcaaaccaa
     9421 ttgttgcaat tggtggatgc gtaaagacga ctgtcggaac aagtttatag tcaagataag
     9481 aatctttctt tccattaaat aaacgttctg ataaacgtct accagctgcg attgcaacag
     9541 gagtcagatc taatttaccg atcacatcac caacagcaaa aatatgttca tccgttgtat
     9601 tttggtattt atctactttg ataaaccctt ttttgtccaa ttccacattt gtattttcca
     9661 gacctaattt ttcggtattc ggtacacgtc caccggcaaa aagtacacaa tcaccagtta
     9721 aggtttcacc attttcaaat agcaccgtat attcgtcatt gtttttagta atttctttag
     9781 caatatgatg tgcgtaagtt tttattccag cttcttggta catttggatc aaattgtccg
     9841 aaagcatttt atcaaacgtt ctaagtggtc gttcttttcg atatgcccac atgacttcac
     9901 tacctaaacc acgaaataca cctgaaagtt cagccgcaat gtatccagca cctaaaacga
     9961 ttgctctctt aggtaactct gttaattcaa aaaatccatt tgaatcaatt gcgtattctc
    10021 caccaggaat ggtcatttta cttgcacggc ctccagtagc gatcaagatt ttaggagcac
    10081 gataggtttc accgtttact tcgatcgttt gactatcgat aaaagtagcg taacctttta
    10141 tagcctcgac atgattgcta tctaatcctc tttgataagc accgtgtaga aaatcaatat
    10201 atttttctcg tctttctact agaccggcga aatcaacatt ttttaaatct gcttggatac
    10261 cgtaacttga ggcatctcgt tcaattgttt ccaaaatcgt acttgcttgc cacataactt
    10321 ttttaggtac acaaccgacg ttgacacatg ttccgcccaa ttcattcgct tcaatcagca
    10381 aaacttttgc gccatgcatc cctgctctgt ttgctgaagc aataccgcca gagccgccac
    10441 caattacgat gtaatcatat atttgcattt tgttcctcct tattgaagag atcaatcaaa
    10501 acctttattt aacgatcctt tttctacaga aaacaagtgt aacacttttc ttgaattttg
    10561 tatgcgaaga tgcttaaaac agggatatat cagctttttc cttatttacg ttttcggatc
    10621 atatctccag gtttcaccgg ctgtgcgact ggcatagtta aaatcatcat tggatttggt
    10681 gctcggtcaa tggattcgcc ttcttcatta tgcataactt caacggtttg actgaaatga
    10741 ctgaatcctg gaccataaaa ttcgatttca tctcctacag aaaacaagtt tctctgacgt
    10801 atcgtagcaa tctgtgttgc ctcatcataa gataatactt caccgacaaa tttatatact
    10861 gggattttac gacgttcacc gaataattgt tcgttttcag ttggtgtatt gtagtaaaaa
    10921 cctgtagcta attctcgttg tgctactttc cataactcgt cgatccattc ttgcttgcag
    10981 acatagtttt caggatcttc catataactg tctacagccg ctttatagac atttgacacc
    11041 gtagatacat aatggatcga tttcattctt ccttcgatct tgaagctgtc tacgccgttt
    11101 tcaatcaatt ctggaatatg ttcgatcatt gacatatcca ctgcactcat cgagaattct
    11161 tcttccactt cgcctgtatc agttaaactg tttcgttctt gaccaaaagg catatcgaac
    11221 aatccatact tccagcgaca agattgagaa catccgccac ggtttgcatc ccgcatcgac
    11281 atatggttgg acaatgtgca tcgaccagaa taagagatac acatggcacc atggataaat
    11341 gcttcgattt cggtatccgt atttttacga atctctgcca cttcctccat tgaaacttca
    11401 cgagctaaaa cgacacgttc caacccttct tctttccaga attccaatgt ctcaaaatta
    11461 gttgctgaag cttgagtgga taaatggatc ggcagacctg gggcttctgt cgcacaaatt
    11521 tcgatcaaag ctggatcaga aacaatcact gcagaaatac cgacatcacg caaactgcgg
    11581 aaaaattccc ctgctccatc ttcatttcct tcatgggtga ccatattagc agcaacgtat
    11641 accttcgcat catgttcttt tgcaaaagca actccttcag ccatttcttc ataagtaaaa
    11701 ttacctgcac ggcttcttaa accatatgca ttcccgccaa tatatactgc atctgctcca
    11761 taatggatcg cggtttttaa cttttccagt gttcctgctg gcgctaggat ttctggtcgt
    11821 ttcaattttc tttttactgt catttctttc atcatactcc ttatttgata gcagaaggat
    11881 caatataaaa gaatcctgta tctaattcac ggttttcagg atgcaattta ctgatttcgc
    11941 tagtcatttt ttccgctttt tcttgatccc agctaccttc taaaatttgt ttttttgctt
    12001 cggcgaatag tcgagtgatg tttacgaatt tttcgcctgg agtaaataat ccgtccaatt
    12061 tccatgtaca gtaatgatgc tgataaagtt cattcaattc attcgagaga ttcagatcat
    12121 tgctggcaaa aatatgtgtg ccatgactat cttcataaat agaataatgt gtctcttctt
    12181 ttttaggttc cgagataaaa agacctcttt cacgatcttt ttgttcgtct tgtttcgtat
    12241 agttatagta attctgtagc agtggacgtt tggattgatg gatgcatgta gcaccataca
    12301 caagggtttc tacaggaata tcgagttttt cttccatcgc cttcatctct tcaaaaggaa
    12361 cttcacgagc aagtactgct ccaactgcgc cttttttcgc ccagaaattg atttgacgtg
    12421 cgctcgttac taatgtttca gcatcataaa tatacggtat acgcaattca ggattttttt
    12481 tcatcgtata aataatacct gtatcaccaa ctgtgatcac atctacgttt atttctttta
    12541 aaaaagccaa atattcaggt acatttttca ttttttcagg atgaatgatc ccatttacag
    12601 caatattcac ttttttacca tacttgtgtg ctaattctac caattgtcgc tgttcttctc
    12661 gtgtaaatga agcaggcaga cgcaatccaa attgctcttc accgaaatat agtgtatctg
    12721 cacctgcagc aagcaactgt tctgcttgtt caatagtttc tactgtggtt atgagttcga
    12781 tcataatttt ctcctcctac aacttcataa tagtagcata gctctaaaaa ctttccaata
    12841 cttcttctat ttaaagtcgg atttttgaaa attatttcac aaaaaatcaa aataaagttc
    12901 gatatattct gtccaaatat aaaaagagat tgtgacaaaa atcttgtcac aacctcttaa
    12961 tcaagtctta ctattataaa aattcagcta cacaggccaa gtaactatag ggaacctact
    13021 gtttcctttt taggtccgcg aacattcaat gtgatcttac cttttgaagc acctactgtt
    13081 acgtggtcac ctaaacgaat ttctccgcca agcaaagcct cgctcaatcg atcttcaatt
    13141 tctttttgaa gtgcgcgacg gattggacgt gcaccatatt caggatcaaa tccagctttc
    13201 ccgatcacat cgattgcaga tggtgtgatt ttcacttgaa tatcttgttc tttcaatcgt
    13261 ttgataatcg ctttgctcat gatttggacg attgtatgga tttcatcttt actcaatgaa
    13321 tggaagacga cagtctcatc gattcgattc aagaattcag gtcggaaagc tttctttaac
    13381 tcttctaaga tacgtttttg catggctttg tgatctttag taacatcttg gacattgaat
    13441 ccgacatttt tttcttctcg aatttgtgtc gctccgatat ttgaagtcat aatcatgatt
    13501 gtatttctaa agtccacttt tctaccttta gaatctgtta aatgaccatc atccaatact
    13561 tgcagaagta aattgaaaac gtctggatgt gctttctcca cttcatctaa gagaatcact
    13621 gaatatggtt tttgacgaat tttttctgtc aactgtccac cttcatcata ccctacgtat
    13681 cctggaggag acccgattaa tcggcttgta ctgtattttt ccataaattc tgacatatca
    13741 acacggatca gggcatcttc acttccaaac atgacttctg aaagtgcttt ggccaattct
    13801 gtttttccga caccagttgg tccaaggaac atgaatgaac caattggacg atcaggatct
    13861 ttcaatccgc ttcgtgcacg gcggatcgca cgtgaaactg ctttgacagc ctcatcttga
    13921 ccaacgacac gttcatgcag aagtccttcc agttctaaca agcgttcgct ttcttttttc
    13981 tccaactgct gtagagggac accagtccac tccgatacaa ctgtagcaac atcttcttct
    14041 gttacacgat cagaataacc ttgctcctgt tttgcttcaa ttgcagaaac tttttgcagc
    14101 tttttacgta gtgctttttc ttgtctgcgt aattgagcag cattttcaaa gtcttgtttt
    14161 tggatcgctg cttccttttc ttgtactagt tcatcgattt ctagtttgat cacagttgat
    14221 tttgttaagt gatcggtttg atccaaacgg actttcgctg ctgattcatc gatcaaatca
    14281 attgctttat cgggtagttg acgagaagtg atataacgaa cagaaagatt gactgctgct
    14341 cgtacagctt catctgtgat ctccactcca tggtgctctt catatcttgt acgtaatcct
    14401 tgaagaatca cttctgcttc ttcaggagtt ggctcatcta cttgaatgcg agcaaaacgt
    14461 cgctctaatg cagaatcttt ttcgatatat ttttgatatt catctagggt cgttgctccg
    14521 atcgtttgta attctcctcg agctaacgcc ggtttcaaga tattcgaagc atcaattgct
    14581 ccttctgctc cacctgcacc aattaaggta tgcaattcat caatgaataa gattacttga
    14641 ccatcattat agatttcgtc gatgaccttt ttcattcgat cttcaaattc accgcgatat
    14701 ttcgtaccag caaccaaaga tcccatatca agcatcatca ggcgcttttc ttgcatgtct
    14761 tctggcactt cacctaaaat gattttttga gctaaccctt cagcaatggc tgttttgcct
    14821 acaccaggtt cacccaccag tacagggtta ttctttgtcc gtcggctaag aatctggata
    14881 agccgtttca cttctctcga acgaccaaca actgggtcca gcttgttttc ttttgctaat
    14941 ttcgtcaaat cacgtgctaa tgaatctagc gtaggtgttc cttgcggtgt ttgccgttta
    15001 tcttgtacat ttacatttct acgacgattc attccttttc cagcagaatc accagctagc
    15061 cccatttttt gtttcagaac ttgacgggct tttgaaaggc tgatacctaa gttgatcatg
    15121 attcgagtag ccatgatact ttcatctttt aataagctca tcaaaatatg ttctgttcca
    15181 actttttgag aaccagtttt cttcacttcg ctgctagcta aagcaaaaac ttgtttcatt
    15241 cttggtgaat atggaagaaa gactccttta gggtaagaac ggataccacc ataaccaatc
    15301 agatgttcaa tttcttcata aatttccgtt tcattcaaat ccatttcacg taatactttt
    15361 ccagcaattc cattttgctc gaccactaag gctagtaaaa catgttctga actaactgat
    15421 tgatgtttca accgttttgc ttcttcttgt gcaatttcta atactgcttg ggctctttca
    15481 gtaaaaagtt cttccatcta atctcacttc ccatcttcat aacttaaacg ttccagaaat
    15541 gctcggagaa tccgagcacg tgtatgatct tcatattcat tgaaattcaa tgtgtttttt
    15601 gcaattgcac tcaacatcaa gtttccttct ttttttgtaa ttatttggtc ttcataaagt
    15661 ttttgaataa tagcaaaagc atttttctca ctgattgtat catcaaataa ctgatttatc
    15721 tgctcaagta attgtttttt atctgaaatc cttacttttg caattcgtat ataaccacca
    15781 ccaccacgtt tgctttctac tagataacca cgttggatgg taaaacgggt attaatcaca
    15841 tagttgattt gtgaaggaac acagttaaag aggtctgcca tttcaatcct tcgaatctcg
    15901 atcatttcac tattttccag aattttcttt atatatgatt caattaaatc cgaggtattc
    15961 tgatgtgcca tcttattcat tccctttgac taatattgac cttaattata acgaatttga
    16021 taatagaaag aaagaagaac gctttttgtt aaataaatcc tttagaatca acaaaaaaaa
    16081 atacggaata tactattccg tatc
//
LOCUS       DAZKLD010000061        16047 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.061, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000061 DAZKLD010000000
VERSION     DAZKLD010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16047)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16047)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16047
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.061"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(342..2312)
                     /locus_tag="ACG8KR_001567"
     CDS             complement(342..2312)
                     /locus_tag="ACG8KR_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0200935.1"
                     /translation="MEIMGRSQLLNFKKKRKTKKEQKTRNSKVVNNTFVSDLTSLVGY
                     QFTPSYIRTGKRWGTIVKLVNTYGMNRNYGFGWMVKAIPEVRVEGVKAYLIEADKPVS
                     QKDQQEIMHENVGGSTNSYENNLEGKNSDAEISKTVMRIMDLNTASLLDSMANKIIDW
                     KLEILLVSDSPNKISEQLIKLQRLYDEKMYGLKLMSYGGDQRDLFDRLLSEPIGNVYE
                     YTSMSSDFAGNDHAIRKGLDDANGWAIGTMSTSYASGVAFMALDDSFTSKVLVASHKN
                     ATIKGYDEKISASSLWGQLIANNAMANHKRTFHIVLNKFDYFGDPTGRTFVAKPSLNS
                     VIEKVNLAEEGLNSLELFGNQKDIGDLYRRNLDKLTQIFYLLAGRTLDSATRVLLKKE
                     LGNFYISQKLWDINYEERSKLLVNSSDTMPVLGDFISYLTNAETKAKKSGTEKEVDRI
                     IVLKDTLRTALDSFSRMFNQRTTLKEIGKSDKLQFYYNLGDLRAETNVMEAQFLNTFD
                     FITSQAKKDDIVMIHGFDKLSIETVGLIKKRLTMLEEDGIRLAFLFDTIGSGEDNEYK
                     EDIETADIFNTDGKIYQSFESDFNYTIIGKMTSGELMKYEKKVAPARKGEDDQKLSVD
                     LRQYLTTGRSNKFQIRRPVDLTNNFVIGEFIL"
     gene            complement(2327..3193)
                     /locus_tag="ACG8KR_001568"
     CDS             complement(2327..3193)
                     /locus_tag="ACG8KR_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0200936.1"
                     /translation="MADNSNYGFKHNLHQKPSETIVRVEWLEKHVNSTYSVTAKRLIV
                     SAIVVAVVVLGSIVYLVSDTIKLKFIFVPTLLMIIGFLILISQYMFIDPSSGSLISGS
                     IRLFKSVVNSARVSMGKKKPFRGTEILKVKQDGTIVYRDGSVALMFNLDGATSLTAFP
                     EEVMAQESKSIQYQNTRDRSLAEIKITSSQPQNTERQTRNMKIQKRNARSNKAVFDII
                     DQQERFVSEYIDGVKTTVIQYLILKSQSKMVLDEGVERLENYTSRGLYYSVTQLSAKQ
                     TKEILSDIYALK"
     gene            complement(3183..3416)
                     /locus_tag="ACG8KR_001569"
     CDS             complement(3183..3416)
                     /locus_tag="ACG8KR_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HFQ0200937.1"
                     /translation="MREEKIVYPVIQNNQENMTSSQHLQEAQLVYQVNELPQTNETMS
                     KSISLIGLLIVAIVITVGVYRKLNVKGSISDGR"
     gene            complement(3428..5590)
                     /locus_tag="ACG8KR_001570"
     CDS             complement(3428..5590)
                     /locus_tag="ACG8KR_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0200938.1"
                     /translation="MAKFKDITESINYVAETVPVVALSLKPQLLEVYQKEEKEAKAKK
                     AKVKVWKEFDALLTKDLPKKNYMVIEDVQKLNSEEVIDEDDAILDSEKYVVLLKSSLN
                     YVADLPEKFKEKLLAGVRFMDSSGQLLLIPDDPNMFESEVEEATDFYSAVEQLEVATN
                     GLERLAETFNSSEEVEFEALAEDIPDEKEALFADDDKPTEAENQEVSIEETQTQIQTE
                     TYQQSKIDEKPESQSDKENYVYEVEKIVSESDVPKSLDADFLEKNFEQIIADKFQPVV
                     LDEINDPVDLNEEMLSIAELKPLLFLAKESMNARISIANNKINSELNKTKEQLILKGK
                     QMLADELLRIEQATSLNGQNQFTNTLSVAKESYEAEASKIDDLVMEKSSIIRKDYDMA
                     KQAYLDAELKKLEQRYDAEHLPEVKQQEDEYRKATEESLDQQYQEAIESIKNDALKQN
                     QEAKEVVVERIINSLKPEIENSLTDVQEKTLEIVEKTKSENEAEFHDTKEKITDVVVK
                     YFANKEEVEKQVANNTKELETKLVEQQKTIEEYKDRMKEVKDLAEGYRSNSEKLAEQS
                     EALREDLSRSRSDLAQMAEKTKSLAVATENQSSSKRDFTSKKMATIISVAAMACGTIL
                     GSSYFISHNNQQAQAQTQTSISRVENSIGTNTNSASTNSSYEATPTTEFKVGKDVVIS
                     IDGKTTKAEVISIDGEKAVVKTPDGNQYKIQAHLSDNN"
     gene            complement(5590..5856)
                     /locus_tag="ACG8KR_001571"
     CDS             complement(5590..5856)
                     /locus_tag="ACG8KR_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0200939.1"
                     /translation="MREESKCKMVFDREKMLAHANEVLMSGLKGTELAKIMNMNVNQF
                     YDYRNGSKKIEKARLETLIKFEKAYVYMLDKQKRTIDRKKGVLQ"
     gene            complement(5942..6280)
                     /locus_tag="ACG8KR_001572"
     CDS             complement(5942..6280)
                     /locus_tag="ACG8KR_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0200940.1"
                     /translation="MFDSIKNLVSGMTPDMKTLVQVVLYFLAFVFSLKPGFTAMKFFG
                     DAKWGDGLKYVGAIIAIFIIPVITTVMLYTMGTKTGNDLNQKANIISALIPVVGIYLV
                     SKFPKNEQLG"
     gene            complement(6679..10320)
                     /locus_tag="ACG8KR_001573"
     CDS             complement(6679..10320)
                     /locus_tag="ACG8KR_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3659 domain-containing protein"
                     /protein_id="HFQ0200941.1"
                     /translation="MKFKKYLLQNLRIKRWVVRGLVIGGVLAVTLGAPFGMNDPTPDN
                     QDSKQETVINKNVAYAESKPAEQGMWTLYARILMGDKEVNDSAAKTGQMNLLGQGGIS
                     ADFPYSDISKQGNSIAGGNAGTQLASTLATYSYYGYIETVSGNAIASQASSILGSIGR
                     TIGSFIGSISLILSNIAESINELLGKFFMSMNIFSLMGWGEGGSDVSNPVSKALTSLL
                     NNLGLNNELFKAIASLSMLFFTAILGYQGMKLLSKDGVNSKGLSPVLEKWIVRIFVLF
                     IFLPVAGSLLGVYINGIQSAKKDGDIPTNVVSDYIINTRLWASTQNLAPSSDTGGDVP
                     DGKPTSEIKFVDTDYEPSKKSAMIRAINTSSYARQNNDLDSNSVGMALLKEWSSNDTF
                     NINTYAGDVNVAENDAYMGRAPAVWTGYNLGDGKEGSAIKKPTNLNGYIWSANQNVTD
                     DNRKPDGKGTAHNFDATQPWGVANNATFSTQSVALLLQSALDTSGAKFYAYNIAPSGI
                     QGSAKNMSTVKTEWRTASLVGHGAMGKFGSWLAMNAESIVLAVLYLACAWAMFNVNIV
                     KAFKREFIRIWEVVLTGSLASGAGVMAISLGVVTTSLIGLQLPIFLITFIRAFGDTAV
                     GFIPNTLVGDGVANIVRSAMILGVGYLLVFKKGEKVNNLINMLIGLPLEVAFGFDERA
                     KLLFGNRNDAVRVVDGVSKAIKKSQGAKNPYYDGGNSSVKDMVAGGLGAVGAQKMAER
                     LNNMGDSNADAKSIADKVIPETPEGMSFNGGGVGSTINNTSFKDLVRQDQLRNNSQQY
                     IDHLGRMVDENGNFVDENGEILKDSKYGKDAKGNFIDEKGRLIDEDGNLIDTNGKPLE
                     DSEDGNIGMDSEGRLIDEKGRLVDEDGNLVDENGRRLLNGDFGQDSNGRLVDEKGRLV
                     DEDGNLVDEFGNKLGKHSDGYNVNGDLVDENGNIIETRDALVADRNRELEQQRHTPIA
                     VAIPENEDDEITLDQTHQELRNEMSGEDVSDRVHRELGNGASGESQSRELNSNFDNSA
                     IRNDTALKDRESLSKETDKEQSDVKKKFIPTPNINQTDKYETNNGLGMSKDNQSIREP
                     ELTNDNNRTNNSKSTSDNSGKSNQSTKKPSTAKSAVEYLSKTGKNVYTSADKALAKSP
                     KGSLAGITYEAKNEAIKIGKGTAKVAKATATGASKTGKVVKKSTMSVKDYIVKYGNQK
                     STENKSEDE"
     gene            complement(10323..12272)
                     /locus_tag="ACG8KR_001574"
     CDS             complement(10323..12272)
                     /locus_tag="ACG8KR_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFQ0200942.1"
                     /translation="MENSTEFFKNIQTEFNHALTPMKPPKHKLVGRDDELHELSLILN
                     RPETPQAILLGEAGTGKTALASEWAERQRQDGKKVQVYKLSIGQLRVGGDDVLQTRLE
                     TLIPRLKEYERILKAEDKDNEFVLFIDEFHKVVSSFGSGNKIGGDSLKEPLANESLQM
                     ITATTRKEYENYIKRDDALGRRLQPLELNELKSDVVVDILRQWLNTSSVKDDPQASFR
                     LSQRIPDELLQEIVQANKLYREDLSEPAKSIAVMEDLIAKDRVDGNAYNRESLLQIFG
                     KYNIQLDSDFDMEKLQEELKSIKGQPLAVYTITMLFKRMKLGFDRFKGRKQPKATALF
                     TGSTGVGKTAMAKAVARGLYGDENKVLLVNMPDYKTKDDAMRFRRLIGTHVRHDPSAI
                     IVCDEYEKAGVETLDAFLSILDEGRISFTERGWDDREVHDTVSLSNTILFATTNAGAE
                     MFDSLNRYSSLSRVTNKENSNEYSTELKREYRKVASGIKQALIGEGLKPEVINRFGRI
                     VPFWALSEQTLLEIAYNEMLKSAELVRKHLNIDLRLPPKKERVYGQNFYADDLSMHLV
                     FDMASQEDSKSGGARNILHLVESEFEEAITEAIITYPNAKIFDVKLSEAGAFEGKGDS
                     NAEIIAKPSVAESKENKIVFSIGGA"
     gene            complement(12314..13372)
                     /locus_tag="ACG8KR_001575"
     CDS             complement(12314..13372)
                     /locus_tag="ACG8KR_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliS"
                     /protein_id="HFQ0200943.1"
                     /translation="MEKNVNSTSTKDQYSIIGGLLGDKDKRTRHGLRIMLATFLGVLF
                     LLTGLGSYIYQLGNYNYYLVNNTTPIGSSLEFARSGAEITISDVWTDQNRDVTVVKLG
                     YSQNARKLLSTQGKNYNLYMATKEKDRPKKLEMSYGMLSTDGDAFLFLKGKLEERAYQ
                     VFIANQVSLISGDDTADDDTKVNVNEADSVTKALSQYSMNDVNNEGVISFGKKAKPVN
                     TPDNINFRINPYSDSTNVYQGSFLTKDGDIDYSKVVAVTSKNNVVNKLEDQLNASNTN
                     IKKIEATKAEYLERLQQNPDDKDSQESIRQLDSRMETEIKAINDTQKLIDQYKNADFN
                     KDSFGDMKTTFKYLTLTD"
     gene            complement(13490..13945)
                     /locus_tag="ACG8KR_001576"
     CDS             complement(13490..13945)
                     /locus_tag="ACG8KR_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0200944.1"
                     /translation="MNAINWHEFVNYMESLLDDLKSFSIADAVKHGLPVSTTMKLRGL
                     KIPDEIIKLRTKTIIETAKVVDNMNHELKGKKTTYEKDFERFLKSVIGKYLGKKSFSE
                     LRDEEIVPTPMVSKIRKNKAEGNYYLGTIAKIIDYLENKEQKKGTKKGR"
     gene            complement(14529..15332)
                     /locus_tag="ACG8KR_001577"
     CDS             complement(14529..15332)
                     /locus_tag="ACG8KR_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFQ0200945.1"
                     /translation="MSQEVTEKIIDSDVDLVSEQTNAIVRYNNGLNTTALRGFKPVEM
                     DVFWAILSKMKRQGTAEVTFDFNLFRYLVKYDRREKDSFYSVLKALSDKLGTLTYRFE
                     DSDVYEQLWLFQKFRIDKQKETVTIKANEEFEFILNSIGKNFTRFELDNFTKLRSSYT
                     KELYRHLMQYRNKEWRNGYWVVKVEDFRRALSIPSSYRMTNIDKRILEQAKEEFLAPD
                     ENGIQVFETFDYEKIYAKKGRRVDRLRFTFSEPESNLPTISMHNWLEED"
BASE COUNT         4641 a         3213 c         2265 g         5928 t
ORIGIN      
        1 aaactctcgc tttttcttca tcagtaaagt ttttatacaa ctgattacaa taatgaggtt
       61 tttgaagttt ctgaatacct atactaactt caaatccttc ttcatgttga attaggatta
      121 cttctgtgtc tctacctaga aatattcgat ctaaaatttt tatgtttggt ttcattattt
      181 aacctccact ataattattt cactcagtaa ggtttcactt tatgaaattt ctatagttct
      241 cttttcacaa aacgtaccat gttctctaag actgttttta gtctcaaata tacatgtttt
      301 ttcttatcct ttccttatac actcaaaatc aaagataaaa cttaaagaat aaactctcca
      361 ataacaaaat tattagtcag atccactggt ctcctgatct gaaatttatt acttcttcct
      421 gttgtcaaat actgtctcaa atcaacagaa agtttttgat catcttctcc ttttctagca
      481 ggagcaactt ttttttcata cttcattaac tctccactgg tcatctttcc aatgatagtg
      541 taattaaaat cactttcaaa actttggtaa atttttccat ctgtattgaa tatatcagct
      601 gtttcaatat cttctttgta ttcattgtct tctcctgaac cgattgtatc aaatagaaag
      661 gcaagacgaa ttccatcttc ttcaagcatt gttaatcttt ttttaattag cccaacggtt
      721 tcaatagata acttatcgaa gccgtgaatc ataacaatat catctttttt tgcttgtgat
      781 gtgatgaaat caaaggtatt taaaaattga gcttccataa cattggtttc tgctcttaga
      841 tcacctaagt tatagtaaaa ctgtaatttg tcacttttac cgatttcttt cagtgtcgtt
      901 ctttgattaa acatcctaga gaagctgtct aatgctgttc tcaatgtgtc ttttaataca
      961 ataatacgat cgacttcttt ttcagtacca gattttttgg cttttgtttc agcattagtc
     1021 aaatagctaa taaaatcccc aagaactggc attgtatcac tactgttcac caaaagtttt
     1081 gaacgttctt cataattaat atcccaaagt ttctgactta tataaaagtt acctaattct
     1141 tttttcaata aaactctagt tgcagaatct aatgttctgc ctgctaacaa ataaaaaatc
     1201 tgagttagtt tatcgagatt ccgtctatat aaatcaccaa tgtccttttg attaccaaaa
     1261 agctccaaag agttaagacc ttcttcagcc aaattaacct tttctattac actgtttaac
     1321 gaaggtttgg caacaaatgt tctaccagtt ggatcaccaa agtaatcaaa tttatttaag
     1381 acaatatgaa aagttcgttt atgatttgcc attgcattat tagcaatcaa ttgcccccaa
     1441 agtgatgatg ctgagatttt ttcatcatag cctttaattg tagcgttttt gtgtgatgcc
     1501 actaaaactt tacttgtaaa agaatcatcc agtgccataa aagctacacc acttgcgtag
     1561 ctagtagaca tagtgccgat agcccatcca ttcgcatcat ctaaaccttt gcgaattgca
     1621 tgatcatttc ctgcaaaatc cgaactcata gacgtatact catagacatt cccgattggt
     1681 tcacttaaaa gtctatcaaa cagatcacgt tgatctcctc catatgacat aagctttaat
     1741 ccatacattt tttcatcata caaccgttgg agtttaatta gctgttcgct gattttatta
     1801 ggagaatcgg atactaatag gatttctagc ttccaatcaa taattttatt tgccatgctg
     1861 tctagtaaag atgctgtatt caaatccata atccgcataa cagttttgct aatttcagca
     1921 tctgaatttt ttccttctaa attattttca taactattcg ttgatccccc aacattctcg
     1981 tgcattattt cttgttgatc tttttgtgac actggcttat ctgcttcaat tagataggct
     2041 tttacccctt ctactctcac ttcaggaata gctttaacca tccaaccaaa tccataattt
     2101 ctattcattc cataagtatt cactaatttc acaattgttc cccaccgctt acctgtccta
     2161 atgtaagatg gtgtaaattg ataaccaact aaagatgtta aatcactaac aaaggtatta
     2221 ttcactacct ttgaatttct agttttttgt tcttttttag tttttctctt ttttttgaaa
     2281 tttaatagtt gtgatctacc cataatttcc atttaatctc tcacctctat ttcagtgcat
     2341 aaatatcaga taatatttct tttgtttgtt tagctgacaa ctgagtgaca ctataataaa
     2401 gtcctctact cgtatagttt tctaaacgtt ccacaccttc atccaatacc atcttagatt
     2461 gagatttcaa gataagatat tggattactg ttgttttcac accatcaata tattctgata
     2521 caaatcgttc ttgctgatct attatgtcaa agactgcttt gttactcctt gcatttcgtt
     2581 tttgtatttt catatttcga gtttgtcttt ccgtattctg aggttgagat gaagtgattt
     2641 tgatttcagc taaggaacga tctctggtat tttgatattg tattgatttc gattcctgag
     2701 ccataacttc ttctggaaaa gcggttagtg atgtagcccc atctagattg aacattaatg
     2761 ctacactccc atctcgatat acgattgttc catcttgttt cacttttaaa atctctgtgc
     2821 ctcgaaatgg tttctttttc cccatagata ctcttgctga attaactact gatttgaaca
     2881 gtctaataga accagaaatc aatgaaccac tagatggatc aataaacata tactggctaa
     2941 ttagaataag aaatccaatt atcattaata acgttggaac aaaaataaat ttcagtttaa
     3001 tcgtatcaga aactaagtaa acaattgatc caagaacaac gactgcaaca actatagctg
     3061 atacaattaa tctttttgct gtaacagaat aggtgctatt gacatgcttt tcaagccatt
     3121 caactctgac aattgtttca gaaggttttt gatgtaagtt atgtttaaat ccatagtttg
     3181 agttatctgc catcagaaat acttcctttc acgttcaact tacggtaaac acctacagtt
     3241 atcactattg caacaataag caagccaatt aaagatattg acttgctcat tgtctcgttc
     3301 gtttgtggta gttcatttac ctgatacacg agctgtgctt cctgtaagtg ctgagaagag
     3361 gtcatgtttt cttgattgtt ttgaattact ggataaacta ttttttcttc tcgcataata
     3421 gccctcctta gttattatca gataagtgag cttgaatttt gtactgattt ccgtctggag
     3481 ttttcacaac tgctttttct ccatcaatag aaatcacttc agccttagtt gttttcccat
     3541 caatactgat gacaacatct ttcccaactt tgaactctgt agttggtgtt gcttcatagc
     3601 tactatttgt tgaagcagag ttcgtatttg tcccaatcga attttccact ctcgaaatag
     3661 atgtttgagt ttgagcctgt gcttgttgat tattatgact aataaagtag gacgatccca
     3721 agatcgttcc gcaagccatt gcagcgacag aaatgatagt agccatcttt ttacttgtaa
     3781 aatcacgttt tgaacttgat tgattttctg tagcaactgc taatgatttc gttttttctg
     3841 ccatttgagc tagatcactt cgggaacgag ataagtcttc tcttaaagct tcactttgtt
     3901 ctgccaactt ttcgctattt gatctataac cctctgctaa atctttgacc tctttcattc
     3961 gatctttata ttcttcgata gttttttgtt gctctacaag ttttgtttct aattctttcg
     4021 tgttgttagc tacttgcttt tcaacttctt ctttgttcgc aaaatatttg acaaccacat
     4081 ctgttatttt ctcttttgta tcatggaact cagcttcatt ttctgacttt gttttttcaa
     4141 caatctctaa cgttttttct tgcacatctg ttaatgagtt ttcaatttct ggtttcaatg
     4201 aattgataat ccgttccacc acaacttctt ttgcttcttg gttttgtttc aatgcatcgt
     4261 ttttaataga ttcaattgct tcttggtact gttgatcaag actttcttca gttgcttttc
     4321 tatattcatc ttcttgttgt ttaacttctg gtaaatgttc agcatcatac cgttgctcta
     4381 gtttctttag ttcagcatcc aaataagcct gtttcgccat atcataatct ttacgaatga
     4441 tactggattt ttccatcact aaatcatcaa tcttagatgc ttctgcttcg tagctctctt
     4501 tagcaaccga taatgtattt gtaaactgat tttgaccatt taatgaggtt gcttgctcta
     4561 tccgcaaaag ctcatcggct aacatttgct ttcctttgag gatcaattgc tccttcgtct
     4621 tatttaattc actgttgatt ttattattag cgatagaaat acgagcattc atactctctt
     4681 tagctaaaaa cagcaatggt ttcaactcag ctatggatag catctcctcg tttaaatcaa
     4741 caggatcatt aatctcgtct aaaactacag gttgaaattt atcagcgatg atctgttcaa
     4801 aatttttttc taaaaaatca gcatctaagc ttttcggtac atcactttcg gatacgattt
     4861 tttctacttc ataaacataa ttttctttat cactctggga ttcgggtttt tcatcaattt
     4921 ttgattgttg gtaggtttct gtttgtattt gagtttgtgt ttcttctatg cttacttctt
     4981 gattttctgc ttcggttggt ttgtcatcat ctgcaaaaag agcttctttt tcatctggta
     5041 tgtcttctgc taaggcttca aattcaactt cttcagaaga gttgaaggtc tcagcaagtc
     5101 gttctaatcc atttgtagct acctctaatt gctccactgc gctatagaag tctgtagctt
     5161 cttcaacttc tgattcaaac atattaggat catccggaat aagtaataat tgaccactac
     5221 tatccataaa acggacacct gctaatagct tctctttaaa tttttctggt agatcagcta
     5281 cataattcaa acttgacttc aatagaacga catacttttc ggaatcgaga atagcatcat
     5341 cctcatctat aacttcctca ctgttgagct tttgtacatc ttcaattacc atataatttt
     5401 tctttggtaa atctttggtc agcaacgcat cgaattcttt ccataccttt accttagctt
     5461 tttttgcctt cgcttctttt tcttcttttt ggtacacttc gagtaattgt ggcttcaaag
     5521 agagtgcaac tacaggaact gtttctgcca cataattaat agattcggta atatctttga
     5581 attttgccat tattgtaaaa ctcctttttt tcggtcaata gttcttttct gtttgtctag
     5641 catatataca tatgcttttt cgaatttaat aagcgtttca agtctggctt tttcaatctt
     5701 tttactccca ttccgataat catagaactg gttaacattc atattcatta tctttgccag
     5761 ttcggtacct ttcagaccgc tcattaatac ttcgttggcg tgagctaaca ttttttctct
     5821 atcaaaaacc atcttacatt tactttcttc cctcacttct aaactacgct tggagcgtag
     5881 ttttgtggta aaaagtaaat tgaacaaatc ggcatattat ccaaatttgt tcaattcact
     5941 tttaacctaa ctgttcattt tttggaaatt ttgaaactag atagatacca acaactggaa
     6001 taagtgctga tataatatta gctttttggt tcaaatcatt acctgtttta gtacccattg
     6061 tgtaaagcat aacggttgta attacaggga taataaagat tgcaataatc gccccaacat
     6121 attttaatcc atctccccat tttgcatcac caaaaaattt cattgcagtg aatcctggtt
     6181 tcaacgaaaa aacgaatgcc aagaaataca aaacaacttg tactaaagtt ttcatatcag
     6241 gcgtcattcc gctaaccaaa tttttaatag aatcgaacat ataaaaacct cctttttaat
     6301 atttttaata agttaattat atcatcgaca aatcttattt gttcacttgt caatgatcta
     6361 actaagaaaa agacgtctcg atatttacat ctttatgtta tcataataac ataaattttt
     6421 gatgattaaa agagattttc cattaaatta tttcattttt tttattggct aaaaatgctg
     6481 gtttatcaag tttttgttta gataataatt ctgttcttta ccagcataag ccctttttaa
     6541 ttaaacaact ttgaacagta aatatttaat tcatttatag ataattttaa aaattttttt
     6601 gcataaatag aaatttttct tgacatcttc agttaaaaat aaaagtaaaa agaaggagca
     6661 aatcaatgct ccttcttttt attcgtcttc agacttattt tcagttgatt tctgattacc
     6721 gtattttaca atatagtcct tgactgacat ggtagatttc ttcactactt taccagtctt
     6781 ggatgctcct gtagcagtag ccttggcaac cttagccgtt cctttaccta ttttaatagc
     6841 ttcgttttta gcttcatagg ttatccctgc tagactacct tttggagatt ttgctaaagc
     6901 tttatctgct gaggtgtaaa catttttacc agttttcgat agatattcaa cggctgattt
     6961 ggctgttgat ggtttcttgg ttgattgatt agatttccct gaattatctg aagtagattt
     7021 agaattattt gttctattat tatcatttgt cagttcaggt tctctaattg attgattgtc
     7081 tttcgacatt cctaatccat tatttgtttc atatttatct gtttgattaa tattaggagt
     7141 aggaatgaac tttttcttca catctgattg ttctttatcc gtttctttcg atagcgattc
     7201 tctgtcttta agtgctgtgt cattccgaat agcactatta tcaaagttac tgttaagctc
     7261 tcttgactga ctttcaccag atgctccatt tcctaattcc cgatgcactc tgtctgacac
     7321 atcctcacca ctcatttcat ttcttagttc ttgatgagtt tgatctaatg taatctcgtc
     7381 atcttcattc tcaggaattg caacagcaat tggcgtatgc cgttgttgtt cgagttctct
     7441 atttcgatct gccacaagcg catctctcgt ctcaatgata ttcccattct catcgactaa
     7501 atcgccattc acattatatc catcgctgtg tttaccaagt ttattaccaa actcatcaac
     7561 aagatttcca tcttcatcaa ctaaacgccc tttttcgtca actaaacgtc cgttactatc
     7621 ctgaccaaaa tcaccattta gcaacctgcg tccattttcg tcaacaagat ttccatcttc
     7681 atcaactaag cgaccttttt catcaattaa acgtccttcg ctatccatac caatattacc
     7741 atcttcacta tcttccaatg gtttaccatt agtatctata aggtttccat cttcatcgat
     7801 caaacgccct ttttcgtcaa taaagttgcc tttagcatct ttaccgtatt tactatcttt
     7861 taatatttca ccattttcat cgacaaagtt tccgttttca tcaaccatac gtcctaaatg
     7921 atcaatatat tgttgggagt tattacgtag ttgatcttga cgaactaaat ctttgaaaga
     7981 ggtattattg attgttgaac caactccgcc accgttgaat gacattcctt ctggtgtttc
     8041 aggaattact ttatcagcaa tggacttagc atcagcattg ctatctccca tattgttcaa
     8101 acgttcagcc attttttgcg ctccaactgc tccaagacct cctgcaacca tatcttttac
     8161 tgagctgttg ccaccatcat aatagggatt tttagctcct tggcttttct tgattgcttt
     8221 agaaacgccg tccacaactc gcacagcatc atttctatta ccaaataata acttagcacg
     8281 ttcatcaaac ccaaacgcta cttcaagtgg taagccaata agcatattaa tcaaattatt
     8341 tactttttcg ccttttttaa aaactaaaag atatcctaca cctaaaatca tagcagatcg
     8401 aacaatattt gctacgccat cgccaactaa tgtattagga atgaatccca cagcagtatc
     8461 accaaatgca cgaataaaag taatcagaaa gataggtaat tgtagtccga tcagagatgt
     8521 tgtaacaaca cctaatgaaa ttgccatcac acctgctcct gatgcaagtg atcctgttag
     8581 aactacttcc caaatccgaa taaattcacg tttaaatgct ttcacgatat tcacattgaa
     8641 catcgcccaa gcacatgcta agtaaagaac tgctaaaacg attgattcag cgttcatcgc
     8701 tagccacgag ccaaacttac ccatcgctcc atgaccaact aaggatgctg tacgccattc
     8761 tgtttttact gttgacatat tcttagcact gccttgaatt cctgaaggag cgatattata
     8821 cgcataaaac ttagctcctg atgtatcaag tgccgattgg agcaataaag ctaccgactg
     8881 tgtgctaaaa gttgcattgt tagcaacgcc ccaaggttga gtagcatcaa agttatgcgc
     8941 tgttcctttt ccatcaggct tgcgattgtc gtctgtcaca ttttgatttg ctgaccaaat
     9001 atatccattt aaattggttg gtttcttaat tgcacttcct tcttttccat cccctaaatt
     9061 atagcctgtc caaacagcag gcgctcttcc catataagca tcattttcgg cgacattaac
     9121 atcaccggca taagtattaa tattgaaagt atcattcgat gaccattctt ttaatagagc
     9181 cataccgact gaattagaat ctagatcatt attttgtctg gcataactgc tagtattgat
     9241 cgctcttatc attgcagatt ttttagatgg ttcataatct gtatcaacga acttgatttc
     9301 ggatgtaggc tttccgtcag gtacatcacc accagtatca gatgatggag caagattttg
     9361 agtagaagcc caaagtcttg tgttaatgat gtagtcagaa acaacatttg ttggtatatc
     9421 tccatctttt tttgctgact ggatgccgtt gatataaact cccaatagcg atcctgccac
     9481 tggtaagaat ataaacaata caaaaatacg aacaatccat ttttcaagta ctggtgataa
     9541 tcccttcgag ttaacaccat ctttcgacaa taatttcatt ccttgataac ctagtattgc
     9601 tgtgaaaaag agcatagata agcttgctat cgctttaaat aattcattat ttaatcccaa
     9661 attgttaagc aagctagtta atgctttaga taccggatta gatacatccg atccaccttc
     9721 tccccacccc ataagagaaa atatattcat actcatgaaa aattttccaa ggagttcatt
     9781 tatggattca gcaatattag aaaggattaa actaattgat ccgataaaac taccaatcgt
     9841 cctaccaata cttcctagaa tggatgatgc ttgacttgca atagcatttc ctgagactgt
     9901 ttcaatatat ccgtagtaac tataagttgc taaagtagat gctagttgcg tacctgcatt
     9961 accccctgca atcgaattac cttgtttaga aatgtcagaa taagggaaat cggcactgat
    10021 accaccttgt cctaacaaat tcatttgtcc agttttagcg gcactatcat tgacttcttt
    10081 atctcccatt aggatacgag catatagtgt ccacattcct tgttctgccg gtttactttc
    10141 cgcataagca acgttcttat ttataactgt ttcttgtttg ctatcttgat tgtcaggagt
    10201 tggatcattc attccaaaag gagcgcctaa agtaactgct aatactcctc caataactag
    10261 acctcttacg acccatcgtt ttattctaag gttttgtagt aaatatttct tgaatttcat
    10321 agctacgccc ctccaatcga aaatacaatc ttgttttctt tactttcagc aacggaaggc
    10381 tttgcaataa tttcagcatt agaatcacct tttccttcaa acgctccggc ttcactcaac
    10441 ttaacatcga atatttttgc gtttggataa gtaattattg cttcagtaat cgcttcttca
    10501 aactcagatt caactaaatg caagatattc cttgcacccc ctgatttaga atcctcttgt
    10561 gaagccatgt caaaaactaa gtgcatggag agatcgtctg cataaaaatt ttgaccatat
    10621 actctttctt tcttaggtgg aagacgtaaa tctatattta aatgttttcg tactaattca
    10681 gcactcttca acatttcatt ataagcaatt tccagaagag tttgttcgga taaagcccaa
    10741 aatggaacta ttcgtccaaa tcggttaata acttcaggct ttagaccttc accaatcaag
    10801 gcttgtttga tccctgatgc gacttttcgg tattctcttt ttagttcagt gctgtattca
    10861 ttagaatttt ccttgtttgt aacacgagat agtgagctat atcggtttaa cgaatcgaac
    10921 atctccgcac ctgcatttgt tgtcgcaaat aaaattgtat ttgacaatga aacggtgtcg
    10981 tgaacttctc gatcatccca tcctctttca gtaaaactta tcctaccttc atctaaaatt
    11041 gataaaaagg catcgagtgt ttctacacca gctttctcat attcatcaca cacaataatc
    11101 gcagaaggat catgccttac atgagtacct ataagtcgtc taaaacgcat tgcatcatct
    11161 ttggttttgt agtccggcat attaactaag agaactttat tttcatcgcc ataaagtcct
    11221 cttgccacgg ctttagccat tgcagtttta ccgacccctg ttgatcctgt aaacagagca
    11281 gttgcttttg gttgctttcg acctttaaaa cgatcaaatc caagcttcat acgtttaaac
    11341 aacattgtta tagtataaac tgctaacggt tgtcctttta tggactttaa ttcctcttga
    11401 agtttttcca tatcaaaatc agaatctagc tgaatattat acttaccaaa tatttgcaat
    11461 agactttctc gattataagc atttccgtct actcgatctt ttgcaatcaa atcctccatt
    11521 acagcaattg atttagccgg ttcggacaaa tcctcacgat aaagtttgtt tgcttgtacg
    11581 atttcttgaa gtaattcatc aggaatcctt tgcgataatc taaaagaagc ctgaggatca
    11641 tctttaacag aggatgtatt tagccattgt ctcaaaatat cgacgacaac atcgcttttt
    11701 agttcattca attctaacgg ttgtagccgt ctccctaagg catcatctct tttgatatag
    11761 ttttcatatt cttttcttgt agtagcggta atcatttgaa gtgattcatt tgctaacggt
    11821 tcttttagcg aatcaccccc aattttattg ccactcccaa aagagctaac aactttatga
    11881 aactcgtcaa tgaagaggac aaattcatta tctttgtcct cagcttttaa aattcgctca
    11941 tattctttca atcttggaat taatgtttct aaacgtgttt ggaggacatc atctccccca
    12001 acacgcaatt gtccgattga taatttatat acttgtactt tctttccatc ttgtcgctgt
    12061 ctttcagccc attcacttgc aagtgctgtt ttacctgttc cggcttcacc taacaagata
    12121 gcttgcggtg tttccggtct attcaagatc agtgacaact catggagttc gtcatcacgt
    12181 cccactaact tatgttttgg tggtttcatt ggtgtgagag catgattaaa ttcggtttga
    12241 atattcttaa aaaactcggt actattttcc atctgtttgt gttcacttcc tctaaaataa
    12301 gaataattta cttctagtca gtcaaagtta ggtacttaaa tgtcgtcttc atgtctccaa
    12361 aagaatcttt gttaaagtca gcatttttgt attgatcaat caatttttgt gtatcattga
    12421 ttgctttaat ttcggtctcc attcgactgt ctagttgacg aatactttct tgcgaatctt
    12481 tatcgtcagg attttgttgt aaacgttcca gatactcagc ttttgtggct tcaattttct
    12541 tgatgttagt atttgaagca tttaactgat cttctagctt atttacaaca ttattcttac
    12601 ttgtaactgc tacaactttt gaataatcaa tatcgccatc tttggtaaga aagcttcctt
    12661 gatagacatt agttgaatct gaatatggat ttattctgaa attgatatta tcaggagtat
    12721 taacaggttt tgcttttttc ccaaaagaaa ttactccttc attattaaca tcattcatag
    12781 aatattgaga aagtgctttt gtaactgaat ctgcttcatt cacattgact ttggtatcat
    12841 cgtcagctgt atcatcacca gaaattagtg atacttggtt tgcaataaaa acttgataag
    12901 ctctttcctc taatttacct tttaggaata agaaagcatc cccatcagta cttaacatcc
    12961 catagctcat ttcaagcttc ttaggacgat ctttttcttt tgttgccata tataagttat
    13021 agttttttcc ttgcgtagat aacaactttc tagcgttttg ggagtaacca agttttacta
    13081 ctgttacatc tctattttga tctgtccaca catctgaaat agtgatttct gctcctgatc
    13141 gagcaaactc caatgaagac cctataggtg ttgtattgtt cacgagataa taattatagt
    13201 ttcctaattg ataaatgtag ctacccaagc ctgttaataa aaaaagcact cctagaaaag
    13261 ttgcaagcat aatgcgtaat ccgtgacgtg ttctcttatc cttatcacca agcaaccctc
    13321 caataatact gtattgatct ttggtagatg ttgaattgac gtttttttcc atcagtttgt
    13381 ctgccccttt cataaaagag caaacaatca gacgtttttt cggtaattct aggttcggga
    13441 ttttgaatca tttcggtata ttcttcccca ttaatattta acacaaaaat tatcttcctt
    13501 tcttcgttcc tttcttttgc tctttatttt ccaaataatc aatgattttt gctattgtac
    13561 caagatagta attcccctca gctttgtttt ttcggatttt gctaaccatt ggagtaggta
    13621 caatctcttc atctctcaat tcactgaaag attttttacc tagatatttt ccaataacag
    13681 attttaaaaa acgttcaaaa tctttctcgt aagttgtttt cttgcctttt agttcatggt
    13741 tcatattgtc aacgactttt gcggtttcaa taattgtttt agttctcagc ttaataattt
    13801 catctggaat tttcaatcct cgtaatttca tcgttgtact aactggtaac ccatgcttta
    13861 ctgcatcagc aatactaaaa ctttttagat catctagtaa gctctccata tagttaacaa
    13921 attcatgcca attaatagca ttcattgtct caccccttca tttttttaaa atgaggttcg
    13981 ggcgtaacac atttatgtta ttataatatc ataataacat aaataaatca atcagcaaat
    14041 ttagatatcc cttaaagtat cagcaattat tcaacaagat atttatttta gctatttcag
    14101 tcatcataaa agactaatta gcaataaaat aactaatatt atccatttat cagacgaaaa
    14161 gaaaaaactt atctaattac tctatattaa cttttaacaa atagttgact tgtccaaaaa
    14221 tgttaccttc aatctcacaa agatattaag atgtttccta ccaaaactga tatcaccgga
    14281 ctttttattc tgtgttgtcc aaaagtgtta cctttagtcg atcaacaaat taaaaaaaat
    14341 tgtttttttc ttgacttgtc caaaagtgtt acccatagtt gttaaaattt gtatagatta
    14401 gccattcttt cgaatattat ttagactgaa acttgcttac aaaaaaactt cttgcattta
    14461 ttgtccaaaa gtgttaccca tactttttta aagaagcagg ttgtattaaa attacaacct
    14521 gcttcttctc aatcttcttc taaccaatta tgcattgaaa tggttggcaa gtttgattct
    14581 ggctcactaa aagtgaatct gagccgatca actcttcttc ctttctttgc atagattttt
    14641 tcataatcaa aagtttcaaa tacctgtatt ccgttttcat caggagctaa aaattcttct
    14701 ttcgcctgct ctagtattct tttgtcaata tttgtcattc tataacttga aggtatactt
    14761 aatgctcttc taaaatcttc aactttgact acccaataac catttcgcca ctctttattt
    14821 ctgtattgca tgagatgacg atacaattct tttgtgtagc tacttcttag ttttgtaaaa
    14881 ttatcaagtt caaaacgagt aaagtttttg ccaattgagt ttaagataaa ttcaaattcc
    14941 tcatttgcct taattgtaac tgtttccttc tgtttatcta tccggaattt ttgaaaaagc
    15001 cacagctgtt catagacgtc tgaatcttca aaacggtagg ttaaagtacc tagtttgtca
    15061 ctcaaagctt ttaacacaga ataaaaactg tccttttctc ttctgtcgta tttgaccaaa
    15121 taacgaaaga ggttgaaatc aaaagttact tctgctgttc cttgacgttt catttttgat
    15181 aaaatagccc aaaacacatc catttcaaca ggcttaaatc ctcgcaaagc tgttgtattc
    15241 agaccattgt tataacgcac aattgcattt gtttgttctg atactaaatc tacatcacta
    15301 tcaataatct tttcggtaac ttcttgagac attgattact ctccaatctt ctttttgcgc
    15361 tcctttaatc atgttaatct ggtactaatt aaaggaaaca attttttctg cttgttacct
    15421 aaattaaatc acaactaaag gtaacagtca acaaagattt gttactaatc taattttcta
    15481 ttttttcgcc ttccaaatca agaaaaccga aggtaacgtc ttatcctagt gtgatacctc
    15541 aaatgtccac ttttgttacc tttgctgtcc aaaagtgtta cccttactgt ccacttttgt
    15601 tacctttact gtccaaaagt gttaccttta taccccttta atacttactc tcccaagaga
    15661 ttaagcctcc cctaacagat atattaacag atatttaaca gatatattaa cagatattta
    15721 ataaaggagg attactcctc tcttatttca aaatcttaaa aataaaaaat cttaaaaaaa
    15781 actaacacta tctaggagtt tattttgctt gtgtaacttt gaataattga aaaaaaggaa
    15841 aaggaggaag cttcagcttc cccttttatt tatatttata atatatattt taatctttaa
    15901 aactttagca accttcaaaa aggctttata tcaatcttaa atagatttta gggtggacat
    15961 aaagcgatgc gaacatggac aaaaagcgat gtgaatgtgg acaaaaagcg atgcaaacgt
    16021 ggacaaaaag cgatgtggta ttccgaa
//
LOCUS       DAZKLD010000062        16047 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.062, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000062 DAZKLD010000000
VERSION     DAZKLD010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16047)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16047)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16047
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.062"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(83..802)
                     /locus_tag="ACG8KR_001578"
     CDS             complement(83..802)
                     /locus_tag="ACG8KR_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0200946.1"
                     /translation="MGRKWANIVAKKTAKDANNSRIYAKFGIEIYAAAKSGDPDPHAN
                     QKLRFVIDRAKTYNVPKHIIDRAIEKAKGAGDETYSELRYEGFGPSGSMVIVDTLTNN
                     VNRTAADVRAAFGKNGGNMGVSGAVSYMFDNTAIFAFAGEDADEILEYLMEKDIDVRD
                     VMEEEGQIIVYGEVADFHSIQEALKEKGITDFSIAEIQMIPQNEVTLSPEDQETFEKM
                     IDALEDLEDVQHVFHNVALED"
     gene            complement(933..2084)
                     /locus_tag="ACG8KR_001579"
     CDS             complement(933..2084)
                     /locus_tag="ACG8KR_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HFQ0200947.1"
                     /translation="MKSCGIIVEYNPFHNGHRYHVEMARKITGAEVVIAVMSGNFLQR
                     GEPAIIDKWHRANEALQNGVDLVVELPVEWAVQSADYFAKGAVKILQTLDCDSLCFGT
                     EEETSFDYSSFGRFVQENQDRIEEAFQELSDQTYSYPQKMTEVFRSLYPDITLDFSSP
                     NHILGLSYAKENAKYVHPMELHPIRRKSAGYHEKIITDQRIASATAIRHALEEGKRIA
                     GLVPPQTEEDLSTCSLQTWESYWPYLRYRLLSSTLLELTDIYQMTEGIESRMKTAAAT
                     ADSFEDFVSKVKTKRYTWTRIQRLSCYVLLNIKKQEIPVQQNKNYLRLLGFTDAGRRF
                     LKEKKEISIYSRIGKKEAQEAELLVRSDQIYQLGGNIPEQNFGQIPIRV"
     gene            complement(2301..3041)
                     /locus_tag="ACG8KR_001580"
     CDS             complement(2301..3041)
                     /locus_tag="ACG8KR_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFQ0200948.1"
                     /translation="MAYETFAFIYDEVMDETLYQQWLAFSKRHLQEDTKNILELACGT
                     GALAVAFAKDGYKVTGLDLSEEMLMIASQRAYEEDASIQFVEGNMLDLSEIGQYQAIT
                     CFSDSLCYMKNRQEVQQVFDEVYQALEENGRFLFDVHSTYQVDEVFPEYSYHYQGDNF
                     AFLWDSYPGSEKHSIEHFLTFFVEEQEGSGRFIREDELHQERTYPLESYLRMLENSGF
                     SKVEAFSDFTDEAASEKSKRWFFVAYKD"
     gene            complement(3045..3368)
                     /gene="rsfS"
                     /locus_tag="ACG8KR_001581"
     CDS             complement(3045..3368)
                     /gene="rsfS"
                     /locus_tag="ACG8KR_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HFQ0200949.1"
                     /translation="MEAADSKRAEDIIALDVRNVSLLADYFMICSANSERQINAITEE
                     IIDKEEENKYEVKRIEGKEGGKWVLIDLGDVIVHVFHAPERNFYNLEKLWSDAPLVDL
                     SEWLD"
     gene            complement(3425..4018)
                     /gene="yqeK"
                     /locus_tag="ACG8KR_001582"
     CDS             complement(3425..4018)
                     /gene="yqeK"
                     /locus_tag="ACG8KR_001582"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HFQ0200950.1"
                     /translation="MNMDYSNEYTAYSRENLMQKIQMRMSEQRFKHVLGVEETAVALA
                     KKYGASPEKASIAALTHDYAKERPDEEFKMVIVRDGFDPELLNYNNAIWHGLVGASFV
                     ERELGITDAEILHAIRVHTTGAAKMSLLDKIIYVADYIEPGRDFPGVQDARAIAWADL
                     DEAVAFETKHTLAHLLAQEQQIYPKTIETYNYWVAKK"
     gene            complement(4002..4652)
                     /locus_tag="ACG8KR_001583"
     CDS             complement(4002..4652)
                     /locus_tag="ACG8KR_001583"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HFQ0200951.1"
                     /translation="MMNTQAKTFVRSQLFPEEMPQFLEKKKQVGILGGTFNPVHLAHL
                     VMAEQAGRNLGLDRVFLMPSYQPPHVDEKQTIDAKHRLNMLELAVEDNPFLQIETIEL
                     ARGGKSYTYDTMKELTQNNPDTDYYFIIGGDMVEYLPKWYKIDELTSMVNFVGIRRPG
                     YTTDTPYPVIWVDVPEIDISSTKIRQKIKEGCSIRYLVPDKVIDYIQNEGLYEYGL"
     gene            complement(4662..4973)
                     /gene="yhbY"
                     /locus_tag="ACG8KR_001584"
     CDS             complement(4662..4973)
                     /gene="yhbY"
                     /locus_tag="ACG8KR_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HFQ0200952.1"
                     /translation="MSLRGKQKRFLRSKAHHLQPIFQIGKGGINSAMIVQIEEALEKR
                     ELIKVSLLQNTDEIAEEAAQVLEKEIDCEIVQIIGRVIVLYKPSTKEKYQRLSKEVKA
                     I"
     gene            complement(4989..6098)
                     /gene="yqeH"
                     /locus_tag="ACG8KR_001585"
     CDS             complement(4989..6098)
                     /gene="yqeH"
                     /locus_tag="ACG8KR_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HFQ0200953.1"
                     /translation="MTEELQCIGCGAVIQTNKPGELGYTPQTALEKGLASGEVYCQRC
                     FRLRHYNEIQDVSLTDDDFLRLLNELGQKDALIVNVVDIFDFNGSLIPGLHRFVGDNP
                     VLLVGNKVDILPKSLKKTKLTQWMKERAYEAGIRPVDVLLTSAKKSHEMTELLEKIEE
                     YRDGRDVYIVGVTNVGKSTLINQIIKNTAGVQDVITTSQFPGTTLDKIEIPLDDGHFL
                     IDTPGIIHRHQMAHYLGKKDLRIIAPLKEIKPKVYQLNEGQTLFLGGLARFDFISGNR
                     ASFIAYVSNDVNIHRTKLEKADEFYQKHVGGLLQPPRPDEVEAFPELVRFEFSVKEKT
                     DIVFAGLGWITVTAPVVIAGWAPKGVDVLKRKALI"
     gene            complement(6095..6622)
                     /locus_tag="ACG8KR_001586"
     CDS             complement(6095..6622)
                     /locus_tag="ACG8KR_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HFQ0200954.1"
                     /translation="MFSNYKPTWMVEAIYQITPAQLKNLGIKAVLTDLDNTLIAWNNP
                     DGTEELLTWILEMKNAGIPVVVVSNNNSKRVARAIEKFDLLYVARAMKPLARGINIAK
                     KQLDLADDEIVMVGDQIMTDIRGANRAGIRSILVKPIIATDSWKTQFNRFWEKRIMAY
                     LLAKHPDMGWRGGIE"
     gene            complement(6802..7587)
                     /locus_tag="ACG8KR_001587"
     CDS             complement(6802..7587)
                     /locus_tag="ACG8KR_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HFQ0200955.1"
                     /translation="MDKTAHDVVQLARAKERWTSLDFFEEIFDSFEECHGDRLFADDE
                     AIVGGIARLDGKPVTVIGIQKGRDLQENIRRNFGSPHPEGYRKALRLMKQAEKFNRPV
                     ITLINTAGAYCGVGAEERGEGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALGLAD
                     EVWMLEETIYAVLSPEGFASILWKDGSRAAEAAELMKITAPELRELGIVDRVIPETFN
                     GEQLPRYKVAHIMKKALIERLTELVRLDQHKLLEKRYKRFRQY"
     gene            complement(7580..8449)
                     /gene="accD"
                     /locus_tag="ACG8KR_001588"
     CDS             complement(7580..8449)
                     /gene="accD"
                     /locus_tag="ACG8KR_001588"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HFQ0200956.1"
                     /translation="MALFKKKKYIRINPNRESKPANSPSVPDNMWAKCPNCKHILYTK
                     DIGEEKVCPHCGYAFRIGAWQRLALIIDEKSFEEWDTDLVTKDPLAFPEYTEKIKKMQ
                     DKTGLHEAVLTGKATIQGIPFIIGVMDPNFIMGSMGTIVGEKITRLFERATKESLPVV
                     LFTASGGARMQEGIFSLMQMAKISAAVKRHSNEGLFYLTVLTDPTTGGVTASFAMEGD
                     IILAEPQSLIGFAGRRVIEQTIKQELPEDFQKAEFLLSHGFVDQIVPRMELKQKIHTL
                     LELHTQKGWIDRG"
     gene            complement(8450..9829)
                     /locus_tag="ACG8KR_001589"
     CDS             complement(8450..9829)
                     /locus_tag="ACG8KR_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HFQ0200957.1"
                     /translation="MFSKVLIANRGEIAVRIIRACRELGIQTVAIYSEADAEALHTQL
                     ADEAICIGPAKAVDSYLNVQEVLSAAIVTKAEAIHPGFGFLSENSRFASMCEECNITF
                     IGPKSATIDAMGNKINARQLMQEANVPVIPGSTGVLSTVEEALEIADRIGYPVMLKAA
                     AGGGGKGIRKVQSKEELPQHFSSAQQEAAAAFGNDDMYLEKIIYPARHIEVQILGDRF
                     GHVIHLGERDCSLQRNNQKVLEESPSVVISQTKREALGDAAVRAAKAVNYENAGTIEF
                     LMDEEGDFYFMEMNTRIQVEHPVTEMVTGIDLVKKQVEIAAGEPLNVCQEEVVFQGHA
                     IECRINAENPAFHFAPSPGKIQNLLLPAGGMGLRVDSAVYPGYTIPPYYDSMIAKIIV
                     HGNTRFEALMKMQRALSEFITEGIITNAEFQMDLISHPAVIAGDYSTAFLQEEFLPNW
                     TPETEIGGA"
     gene            complement(9837..10256)
                     /gene="fabZ"
                     /locus_tag="ACG8KR_001590"
     CDS             complement(9837..10256)
                     /gene="fabZ"
                     /locus_tag="ACG8KR_001590"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HFQ0200958.1"
                     /translation="MNIQEIKEIIPHRYPMLLIDRVEEMVEGERIVAKKNVTINEPFF
                     QGHFPEEPVMPGVLIVEAMAQAGAVALLSLEQFKGKTAYFGGLDKAKFRKKVTPGDTL
                     YLEVEILKVKASAGIGKGIAKVDGKKVAEAELTFMIG"
     gene            complement(10281..10757)
                     /gene="accB"
                     /locus_tag="ACG8KR_001591"
     CDS             complement(10281..10757)
                     /gene="accB"
                     /locus_tag="ACG8KR_001591"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HFQ0200959.1"
                     /translation="MDINEIRELVSQFDQSSLTEFDLREGQFELYMNKNKTSRGMNMV
                     QTNETLPVSEPVNVISEQATTAPEIMKEKESAVFDGIEVVSPIVGIVYLQPAPDKPAF
                     KKVGDQVAKGEVICIIEAMKLMNEITSEADGVITEILVENESVVEYGQPLFRIQQK"
     gene            complement(10760..11995)
                     /gene="fabF"
                     /locus_tag="ACG8KR_001592"
     CDS             complement(10760..11995)
                     /gene="fabF"
                     /locus_tag="ACG8KR_001592"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HFQ0200960.1"
                     /translation="MNRVVITGYGVTSPIGNDADSFLNSLKEGTNGIAPITKFDASET
                     GITLAGEVKDFPYDKYFIKKDSKRMDMFSIYGIYAALEAMEMSGLDKEKMNQDRFGVI
                     IGSGIGGLPTIENQVIRLHEKGAKRVSPMFVPMAIANMAAGNIALRVGAKGICTAIVT
                     ACASGTNSIGEAFRNIKHGYSDVILAGGTEATICEMGIAGFAALTALSESTDPNRGSI
                     PFDENRNGFVMGEGAGVLVLESLEHAQARGAKIYGEIVGYGANCDAYHMTAPTPDGSG
                     ASKAMKLAMEEAGVQPEQVGYINAHGTSTPANDQAEAKAIQIALGENFRDTYVSSTKS
                     MTGHLLGAAGGIEALATLLALEHQFIPPTINVEKQDPEIDLTVVKNESKPASFTYAMS
                     NSLGFGGHNAVLCMKRWEE"
     gene            complement(12014..12751)
                     /gene="fabG"
                     /locus_tag="ACG8KR_001593"
     CDS             complement(12014..12751)
                     /gene="fabG"
                     /locus_tag="ACG8KR_001593"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HFQ0200961.1"
                     /translation="MDVKGKNVFITGSTRGIGKAMALAFAKAGANIILNGRGEIPKEK
                     IEEIEAFGVKCVGVSGDISDYEKAGQMIKEAEEKLGSIHVLVNNAGITNDKLVMRMDA
                     EDFKKCLDINLIGTFNMTQHVLKKMMKQREGAIINLSSVSGLIGNIGQANYAASKAGV
                     VGLTKSVAREAATRGITCNAIAPGFITTDMTEVLADKVKEQAEKQIPMQRFGQVEDIA
                     QTAVFLAQNPYITGQVINVDGGLVMHG"
     gene            complement(12763..13680)
                     /gene="fabD"
                     /locus_tag="ACG8KR_001594"
     CDS             complement(12763..13680)
                     /gene="fabD"
                     /locus_tag="ACG8KR_001594"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFQ0200962.1"
                     /translation="MKTAFLFSGQGAQYQGMGKDLYEEAVVKQTFDEASEILGYDMAE
                     LCFTENERLDQTQYTQPAILTVSIAYYRLLKEHGIIPDAALGLSLGEYSALVASNALS
                     FTEAVALVAKRGAYMTEATPAGSGKMVAVMNAPIETIEESCHEASKYGIVSPANYNTP
                     QQIVIGGEEKAVDEAVLLLKEKGFKRMIPLNVSGPFHTAILEPAAKKLAKDLSQIQFS
                     EPSFPIISNTTTEIMKKETIAGLLEQQVMQPVRFYESIHKLKTIGIEQVIEVGPGKVL
                     SGFMKKIDKTIPVLRVENKQTFDETIAIL"
     gene            complement(13787..14011)
                     /locus_tag="ACG8KR_001595"
     CDS             complement(13787..14011)
                     /locus_tag="ACG8KR_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000035 - acyl binding [Evidence IEA];
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFQ0200963.1"
                     /translation="MVFEKIQEIIAEELGKETEEIKLTTDIQEDLEADSLDLFQIINE
                     IEDEFDVKIETEDGIKTVQDLVTYVEKQTA"
     gene            complement(14064..15026)
                     /locus_tag="ACG8KR_001596"
     CDS             complement(14064..15026)
                     /locus_tag="ACG8KR_001596"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HFQ0200964.1"
                     /translation="MEQFGMITATASVLPEKIITNNDLSKMMDTSDEWISSRTGIRER
                     RCVTNQETSDLCILVAEKLIKQKNLNPKDLDFILVATMSPDYTTPSVAAQVQGKLGAT
                     HAIAFDISAACSGFVYALSMAEKMIRCGSTKGLVIGGETLSKLIDWSDRTTAVLFGDG
                     AGGILLEANPQKKLLKEKLAADGTRSSSLTAGYQQNKNPFYSEDSEGSYYLQMTGRDI
                     FDFAVRDVANNIQEVMKDKPVDYLLLHQANLRIIEKIARKVKMPQEKFLTNMDKYGNT
                     SAASIPILLDEAVSSGKITLGKQQKVIFTGYGGGLTWGSILMEL"
     gene            complement(15036..15476)
                     /locus_tag="ACG8KR_001597"
     CDS             complement(15036..15476)
                     /locus_tag="ACG8KR_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005471259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFQ0200965.1"
                     /translation="MEPDLKTVNDYLVSVFNDILTIEESELKKSQFKDLSITEMHTIE
                     AIGMYKKKTTSEVAKELSITVGTLTTAINKLVKKGYVERIRSEDDRRVVKLGLTKKGK
                     LLFRVHQHFHREMVKNILDGMATEEQHALLDALKNLHDFLQDYK"
     gene            15782..>16047
                     /locus_tag="ACG8KR_001598"
     CDS             15782..>16047
                     /locus_tag="ACG8KR_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFQ0200966.1"
                     /translation="MLKEKMKTLPMLPGVYLMKDRDGKVIYVGKAKRLKNRVSSYFHQ
                     NKQHSKKVLRMIHHITDFDFVVVDTELDALLLECQLIQHYRPFY"
BASE COUNT         4251 a         3628 c         2742 g         5426 t
ORIGIN      
        1 acacaaaaaa gcaagacaat ctgttagtga gattgtcttg cttttcgaaa aagactgaat
       61 acttttcgtt aaaactggtt gcttagtctt ctaatgccac attatggaac acatgctgta
      121 catcttctag atcttccagt gcatcaatca ttttttcaaa tgtttcttga tcttctggag
      181 atagtgttac ctcgttttga gggatcattt ggatctcagc gattgaaaaa tcagtgattc
      241 ctttttcttt taatgcttct tgtatactat ggaaatccgc tacttcaccg taaacgatga
      301 tttgtccttc ttcttccatt acatcgcgaa catcgatgtc tttttccatt aggtattcaa
      361 ggatctcgtc agcgtcttct cctgcaaaag caaagattgc tgtattgtca aacatgtaag
      421 aaaccgctcc ggaaacaccc atgttcccgc catttttccc aaatgctgcc cgtacgtctg
      481 ctgctgttcg attcacattg ttcgtcaatg tatcaacaat caccattgag ccgcttggtc
      541 caaatccttc atagcgcaat tcagagtatg tttcatctcc tgcacctttt gctttttcga
      601 ttgctcggtc aataatatgt tttggtacat tataagtttt agcgcgatca atgacgaaac
      661 gtagtttttg gtttgcatgc ggatcaggat cacctgattt tgctgccgcg tagatttcaa
      721 tcccaaattt tgcataaatc cggctattat tagcatcttt agcagttttc tttgctacga
      781 tatttgccca tttacgtccc atattttcac ttcgctttct gatttgatag tttcataacg
      841 caaaaacgtc ctgttccatt atacttgtga atcacgattc tgtgaagctt tttttctttc
      901 ttccttcatt cttctcattt ttagtcaatt atctatactc ggattgggat ttgcccgaaa
      961 ttctgttccg gaatattgcc tcccaattga tagatttgat cacttctaac taacagttcc
     1021 gcttcttgtg cctctttttt tccgattcgg gaataaatag aaatctcttt tttttctttt
     1081 aagaatcttc ttccagcatc ggtaaatcca agtagtcgaa gataattttt attttgttgg
     1141 acaggaatct cttgcttttt gatattcagc aagacataac aagataatcg ttggatccgt
     1201 gtccatgtat atctttttgt ttttaccttt gacacaaaat cctcaaaact atcagcagtt
     1261 gcagctgctg ttttcatgcg cgattctatt ccttcggtca tctgatagat atctgtcagt
     1321 tccagcaaag tagaagataa caaccgataa cgcaaatacg gccaataaga ttcccaagtc
     1381 tgcagcgaac aagttgaaag atcttcttct gtctgaggcg gcacaagtcc agctattctt
     1441 tttccttctt ctaacgcatg gcgaatggct gttgcactgg ctatcctctg atctgtgatg
     1501 attttttcat gatagcctgc acttttacgt cggatcggat gtaattccat agggtggaca
     1561 tacttggcat tttcctttgc ataacttaat cccaagatat gatttggcga agaaaaatca
     1621 agcgtaatat cgggatacag actgcgaaaa acctcagtca ttttttgcgg ataactatac
     1681 gtttgatcag acagttcttg aaatgcctct tctatcctat cttgattctc ttggacaaag
     1741 cgtccgaaac tgctgtaatc aaacgaagtc tcttcttctg ttccaaaaca caaactgtca
     1801 cagtccagtg tttgcaagat ttttactgca cctttagcaa aatagtcagc tgactgaacc
     1861 gcccattcga cgggaagctc cactactaaa tccacaccat tttgtaaagc ttcattcgcg
     1921 cgatgccatt tatcgatgat agccggttcg cctctttgca aaaaattccc gctcattaca
     1981 gcaatgacga cctctgctcc tgttattttt cgtgccattt ccacatgata gcgatgtcca
     2041 ttgtgaaaag gattgtactc tacgataatc ccgcagcttt tcatgcgcat cctccttcat
     2101 agttatcgat aaaaaagaaa tagagacaag taaaaaaagg caaaaatcgg aaaaaatacc
     2161 cgaactatat aaactattct cgcaaacaca atacttctgc gacgaaaagc tgcactgcaa
     2221 gagcaatgcc tatatcgttc ggatatataa tttatgttga agatttattc ttttcttgtt
     2281 aactcgagca ggcaaaaccg ctagtctttg tatgctacaa agaaccagcg ctttgatttc
     2341 tctgatgctg cttcgtctgt aaaatcagaa aatgcttcga ctttcgaaaa gccgctgttt
     2401 tccagcattc ttaaataact ttccaatgga taggtacgct cttgatgtag ttcatcttca
     2461 cgaataaatc gaccagaccc ttcttgttct tcaacgaaaa aggtaagaaa atgttcgatg
     2521 ctgtgtttct cgcttcctgg ataactatcc cacaaaaaag caaaattatc cccttgataa
     2581 tgatagctat attctggaaa aacctcatct acttgatagg tggagtgtac gtcaaataaa
     2641 aatcggccat tttcttctaa cgcttgatag acttcatcaa acacttgctg tacctcttgt
     2701 ctatttttca tatagcacag cgaatcagaa aaacaggtaa ttgcctgata ttgtccgatt
     2761 tctgataaat cgagcatatt gccttccaca aattggatcg atgcgtcttc ttcatatgct
     2821 cgctggctgg caatcatcaa catctcttct gaaaggtcta accctgtcac tttatatcca
     2881 tcttttgcaa aggcaaccgc taatgctcca gtaccgcatg ccagttccaa aatatttttt
     2941 gtgtcttctt gtaaatgacg tttcgaaaaa gctaaccatt gctgataaag tgtttcgtcc
     3001 atcacttcat cgtaaatgaa agcaaatgtt tcataagcca tatgttaatc caaccattca
     3061 ctaagatcga ctagcggagc atctgaccat agtttttcca gattatagaa atttcgttct
     3121 ggcgcatgga aaacatgtac gatcacatct ccaagatcga tcaataccca ttttccgcct
     3181 tcttttcctt caatacgttt tacttcatat ttgttttctt cttctttatc aatgatttct
     3241 tctgtaattg cgttgatttg acgttcgcta tttgctgaac agatcatgaa ataatctgcc
     3301 aacaaggaaa catttcgaac gtctaaagcg ataatgtctt ctgcccgttt tgaatctgct
     3361 gcttcaatcg caatttttaa ttgttcttta ctatctatga tgatttcctc ctaatatgag
     3421 cagcttattt ttttgctacc cagtaattat atgtttcgat tgtttttgga taaatctgtt
     3481 gttcttgcgc aagcaaatgg gccaatgtat gttttgtttc aaatgctaca gcttcgtcta
     3541 gatcagccca tgcaatggct cgtgcatctt gtaccccagg aaaatctcga cctggttcaa
     3601 tatagtctgc tacataaatg attttgtcaa ggagactcat cttagcagcc ccagtcgtat
     3661 gcacccggat tgcatgaaga atctctgcat cggtgattcc taattcccgc tctacaaacg
     3721 aagcgccaac aagaccatgc cagattgcat tattataatt caataactcc ggatcaaaac
     3781 cgtctcttac gattaccatt ttgaactcct catctggtcg ttcttttgca taatcatgtg
     3841 tcaatgcagc aatactagct ttttcaggag aagcaccgta ttttttggca agagccacgg
     3901 ctgtttcttc tactccaagc acatgcttga agcgttgttc gctcattctc atctgtattt
     3961 tttgcatcag gttttctcta gaataagcag tatattcatt actatagtcc atattcatac
     4021 aacccttcat tttgaatata atcaattact ttatctggaa caaggtagcg gatagaacag
     4081 ccttctttga tcttttgacg tattttcgtg gaactgatgt cgatctcagg tacatctacc
     4141 caaattacag gatatggagt atccgttgtg tatcctggtc gacgtatacc cacaaagttg
     4201 accatagaag tcagctcatc gattttatac cattttggca aatattctac catatcccca
     4261 ccgataataa agtaataatc tgtatccgga ttgttttgcg tcaattcctt catagtatca
     4321 taggtgtaac ttttcccacc acgagctaat tcaatcgttt caatctgcaa aaaaggattg
     4381 tcttccaccg ctaattccaa catgttgagt cgatgttttg catcaatcgt ctgtttctca
     4441 tcaacatgag gtggctgata agaaggcatc aaaaatacgc ggtcaagacc taaattccgg
     4501 ccagcttgtt cagccatcac taaatgtgca aggtgaacgg gattgaatgt tccgcccaag
     4561 atccctactt gctttttctt ctctaagaat tgaggcattt cttcaggaaa taattgtgaa
     4621 cgaacaaatg tcttagcttg cgtattcatc atggtcactc cttatattgc ttttacttct
     4681 tttgatagcc tttgatactt ttcttttgtg gaaggtttgt ataatacaat aacacgtcca
     4741 atgatttgga cgatttcgca atctatttct ttttctaata cttgagctgc ttcttctgca
     4801 atctcatctg tattttgaag taacgatact ttgatcagtt ctcttttttc taaagcttct
     4861 tcaatttgaa cgatcattgc tgaattgatc ccaccttttc cgatttgaaa aataggctga
     4921 agatgatgcg ctttgctacg taaaaaacgc ttttgttttc ctcttaaact cataattttc
     4981 cttcttcctt aaatcaatgc tttgcgtttc aatacatcta caccctttgg cgcccagccc
     5041 gcaattacta ctggcgctgt tacagtgatc cagcctagac cggcaaaaac gatgtctgtt
     5101 ttttctttca cagaaaactc aaaacgaaca agctccggaa atgcctcgac ttcatcagga
     5161 cgaggaggct gtaataatcc acctacatgt ttttgataaa attcgtctgc tttttccagt
     5221 ttcgttcgat gaatatttac atcatttgac acataagcaa taaatgaagc acgattgccg
     5281 ctgataaaat caaatcttgc aagtccgccc aagaaaagtg tttgcccttc attcagctga
     5341 taaactttag gtttgatttc ttttaacggt gcaatgatcc gcaaatcttt tttacctaag
     5401 tagtgcgcca tttgatggcg atggataatt cccggtgtat caatcaaaaa atgcccgtca
     5461 tctaaaggaa tctcgatttt atccaaggtc gttcctggaa actgcgaagt tgtgattaca
     5521 tcttgtactc ccgctgtatt tttgatgatt tgattgataa gcgtcgattt tccgacattt
     5581 gttactccga cgatatagac atcccgtcca tcgcggtatt cttcgatttt ttctaatagt
     5641 tccgtcatct catgagattt cttagcacta gttaataata catctactgg acgaattcct
     5701 gcttcgtaag cacgttcctt catccattgt gttaatttag tttttttcaa tgatttaggt
     5761 aatatatcca ctttgttccc aactaaaagt actggattgt caccgacaaa acggtgtagt
     5821 cctgggatta gtgaaccatt gaagtcaaaa atatccacca cattgacgat caatgcatct
     5881 ttttgcccta attcattcag taagcgcaaa aagtcgtcat ccgtcaatga gacatcttgg
     5941 atctcattat aatggcgtaa tctgaaacaa cgctgacaat agacttcccc ggaagctaaa
     6001 cctttttcca aagccgtttg cggcgtgtat cctaattcac cgggtttgtt tgtttgtata
     6061 acggcaccac agccgataca ttgtaattct tctgtcattc gattccacct cgccagccca
     6121 tatcagggtg ttttgctaat agataggcca taattctttt ttcccaaaaa cgattaaatt
     6181 gtgtcttcca actatctgtt gcgataatgg gttttacaag gatgctacgt atccctgcac
     6241 gattggctcc acgtatatca gtcataatct gatcgcctac cattactatc tcatcatctg
     6301 ctaaatccaa ttgtttcttt gctatgttga tccctcttgc taaaggtttc attgctcgtg
     6361 caacatacaa aagatcaaat ttttcaatcg ctcgcgcgac acgttttgaa ttattgtttg
     6421 aaacaacaac aacagggatt cctgcatttt tcatttctaa aatccaagta agcaactctt
     6481 ctgttccatc tggattattc catgcaatca acgtattgtc taaatcggtc aatactgctt
     6541 tgatgcctag attctttaat tgggcaggtg taatctgata gatggcttct accatccatg
     6601 ttggtttgta atttgaaaac atgctgtctc ctcatctgca aaaaatgaag gagcacataa
     6661 agtactcctc aaccccgtat tatactgcat atcagcaaaa aaaactacct ctatactcgt
     6721 tcgtctttca gaaaaagcat ttctttacaa aatgaccaaa aataaagatt gatcacagaa
     6781 aaatttagaa acaaaaaaca actaatattg gcggaacctc ttgtatcgtt tttccagtaa
     6841 tttatgctga tctaatcgaa caagttccgt caatctttca atcaacgctt ttttcataat
     6901 gtgtgcgact ttatatcgtg gcaactgctc gccgttgaac gtttcaggaa taactcgatc
     6961 aacgattcct aactcgcgta gttctggcgc agtaattttc atcaactcag ctgcttctgc
     7021 ggcgcgacta ccgtctttcc acaaaatcga cgcaaatcct tctggagaaa gcacagcata
     7081 aatcgtttct tccagcatcc agacttcatc cgctaaacct agagcaaggg caccgccact
     7141 tccaccttca ccaatgatga tggcgatgat cggtaccttt aaatcagaca tttcgagcag
     7201 atttctggca atagcctctc cttcgccgcg ctcttctgct cctacaccgc aatatgcacc
     7261 tgctgtattg atcaaggtaa tcaccggtct attgaacttt tccgcttgtt tcatcagtcg
     7321 taaagctttt cgatagcctt caggatgggg agaaccaaaa tttctgcgga tattttcctg
     7381 cagatcccgt cctttttgaa taccgatcac tgttaccggt ttgccatcta atcgagcgat
     7441 tccaccaaca atcgcttcgt catctgcaaa taaacgatca ccatggcatt cctcgaaaga
     7501 atcgaagatc tcttcaaaaa aatccaagct tgtccatcgt tctttagctc ttgctaactg
     7561 aacgacatca tgtgctgtct tatccacgat ctatccatcc tttctgtgta tgcaattcaa
     7621 gaagcgtatg gattttctgt ttcaattcca tgcggggaac aatctgatcg acaaaaccat
     7681 gagataataa gaattcagcc ttttggaaat cttctggaag ttcctgcttg atcgtctgtt
     7741 cgatgacgcg tcttccagca aaaccgatca agctttgggg ttctgctaga ataatatcgc
     7801 cttccattgc aaaactggca gttactcctc cggtcgtcgg gtccgttaaa acagttagat
     7861 aaaaaagacc ttcattgcta tgccgtttga cagcagcaga gatttttgcc atctgcatta
     7921 gagaaaagat tccttcttgc atccgtgcgc cacctgaagc tgtgaacaag acgactggaa
     7981 gactttcctt tgttgctcgt tcgaataatc tggtgatctt ttctccgaca atcgttccca
     8041 tacttcccat gataaaatta ggatccatca ctccaataat gaagggaatc ccctgaatgg
     8101 ttgctttacc agtgaggact gcttcatgaa gtcctgtttt atcttgcatt tttttaattt
     8161 tttccgtata ttcgggaaat gctagcggat ctttggttac aagatccgta tcccattctt
     8221 caaaactttt ttcatcaata atcaaagcta gacgctgcca tgcaccgatt cgaaaagcgt
     8281 atccgcaatg agggcatact ttttcttcac cgatgtcttt cgtataaagg atatgtttgc
     8341 aatttggaca ctttgcccac atgttatcag gaacagatgg cgagttagca ggtttgctct
     8401 ctctattagg attgatccga atatatttct ttttcttaaa cagagccatc tatgcgcctc
     8461 ctatctccgt ttctggtgtc caattcggta aaaattcttc ttgtaaaaat gctgtgctgt
     8521 aatcacctgc tatgactgca ggatgactaa tcaaatccat ctgaaattct gcgttcgtga
     8581 tgattccttc tgtgataaat tcacttaagg ctcgctgcat cttcattaat gcttcaaaac
     8641 gcgtatttcc atggacgatt atttttgcaa tcattgaatc ataatatggc gggatcgtat
     8701 atcccgggta aactgcactg tctactcgca gtcccatccc acctgctgga agcagtaagt
     8761 tttggatctt ccctggagaa ggagcaaaat ggaaagcagg attttctgca ttaatccggc
     8821 attcgattgc gtgtccttgg aatactactt cttcttgaca cacattcaaa ggctcaccgg
     8881 ctgctatctc cacctgtttt tttactaaat cgattcctgt gaccatttct gttactggat
     8941 gttccacttg gattcgagta ttcatttcca tgaaataaaa atcgccttct tcatccatca
     9001 gaaactcgat cgttccggca ttttcataat tgactgcttt agctgctcga acagcagcgt
     9061 ctcctagtgc ttctcttttt gtttgtgaga tcactacaga aggagattct tccagtactt
     9121 tttgattgtt gcgttgtaaa gaacaatctc gctcgcctaa atgaatgaca tgaccaaatc
     9181 gatcacctaa gatctgaact tctatatggc gagcaggata aatgattttt tccaaataca
     9241 tatcatcatt tccaaatgcc gctgctgctt cttgctgagc tgaagaaaaa tgctgtggga
     9301 gctcttcttt tgattgaact ttccgaatgc ctttcccgcc accacctgca gctgctttca
     9361 gcatcactgg ataaccaatt ctgtctgcta tttccaatgc ttcttctact gtagataata
     9421 caccagtact tcctggaatg actgggacat ttgcttcttg catcaattga cgcgcattga
     9481 tcttatttcc cattgcatcg atcgttgcac ttttaggacc aataaatgtg atattgcact
     9541 cttcacacat ggaggcaaat cggctatttt cagataaaaa accaaatcct ggatgaattg
     9601 cttcggcttt tgtcacgatt gcagcgctca gtacttcctg aacattcaga tatgaatcaa
     9661 cggcttttgc cgggccaatg cagattgctt catctgctaa ctgtgtatga agtgcttcgg
     9721 catctgcttc tgaatatatc gctacggttt ggatccctag ttcgcggcat gcacgaatga
     9781 tacgtacagc gatttcaccg cggttagcta tcaatacttt agaaaacata tttcacctat
     9841 ccaatcataa aggttaattc cgcttcggct acttttttac cgtctacttt agcgattcct
     9901 ttgcctattc cggctgaagc tttcactttc aatatctcaa cttcaaggta taaggtatct
     9961 cccggtgtaa ctttcttacg gaattttgct ttgtccaatc cgccgaaata agccgtcttt
    10021 cccttgaatt gttcaagaga aagtaatgca actgctcctg cttgagccat tgcttcaaca
    10081 atcaataccc ctggcataac aggttcttct ggaaaatgtc cttgaaaaaa gggctcattg
    10141 atcgttacat ttttcttagc aacgatccgt tctccttcaa ccatttcttc cactcgatca
    10201 atcaaaagca ttggataacg atgtggaatg atttctttta tttcttgtat gttcattgtt
    10261 ttccctcttt cctgctttgc ttatttttgc tgaatcctaa agagtggctg tccatattcg
    10321 acaactgact cgttttctac aaggatttct gtgatcactc cgtcggcctc actagttatc
    10381 tcattcatca gtttcatcgc ttcaataatg cagataactt ctcctttggc aacttgatca
    10441 cctacttttt tgaaagctgg tttgtcgggc gctggttgca gataaacgat tcccacgatt
    10501 ggcgaaacaa cctcgattcc atcaaatact gccgattctt tttctttcat tatttctgga
    10561 gcagttgttg cttgttcaga tatgacattg actggctccg aaactggcaa cgtttcgttt
    10621 gtttgaacca tattcatgcc tcgacttgtt ttatttttat tcatatacaa ttcaaattga
    10681 ccttcacgca aatcgaattc cgttagcgag gattgatcaa attgactgac taattctctg
    10741 atttcattga tatccaaaat tattcctccc agcgtttcat gcacagaacc gcattatgtc
    10801 cgccaaaccc taatgaatta ctcattgcat aagtaaaact tgcaggtttg ctttcgtttt
    10861 tgacaactgt caagtcaatc tcaggatctt gtttttctac attgatcgta ggtgggataa
    10921 attgatgttc taatgccagc aaagttgcca aggcttcgat cccgccggct gctcccaata
    10981 aatgtccagt catcgatttc gtgctgctga cgtaagtatc tctgaaattt tcacctaatg
    11041 cgatttggat tgcttttgct tctgcctggt cattggctgg tgtacttgtt ccgtgagcat
    11101 tgatatatcc gacttgttcc ggctgtacac cagcttcttc catcgcaagc ttcattgctt
    11161 tagatgcacc agaaccatct ggagtaggag ctgtcatatg gtaagcgtca cagtttgcac
    11221 cgtatccaac gatttctcca tagattttgg caccgcgagc ttgtgcatgt tctaaagatt
    11281 caagaactag aacgcctgct ccttcaccca tcacaaaacc attccgattt tcgtcaaacg
    11341 gaatcgaacc acgattagga tcagttgatt ctgagagtgc tgttaaagct gcaaatccag
    11401 caatccccat ctcacaaatc gttgcttctg ttcctccagc aagaatgaca tcagaatagc
    11461 catgtttgat gttacggaat gcttccccaa ttgaattcgt tccgctagca caagctgtta
    11521 cgatagctgt acaaatacct tttgcaccta cacgcaaagc aatatttcca gctgccatgt
    11581 tggcaatcgc cattggtacg aacattggag aaactcgttt tgcacctttt tcatgtagac
    11641 gaataacttg gttttcgatt gttggaagac caccgatccc agatccgatg atgacgccaa
    11701 accggtcttg attcattttt tctttatcta aaccgctcat ctccattgct tctaaagcag
    11761 cgtagattcc atagatcgaa aacatatcca ttcgtttgct atctttttta ataaaatatt
    11821 tatcgtaagg gaaatctttg acttcacccg ctaaggtaat ccctgtttct gaagcatcaa
    11881 atttagtgat cggtgcgatg ccattcgtcc cctcttttaa gctattcaaa aagctgtctg
    11941 cgtcatttcc aataggtgaa gtgactccat agccagtaat taccactcga ttcatttgtt
    12001 tgctctcctt ttcttatccg tgcatgacca atccgccatc gacattgatg acttgtccgg
    12061 tgatatatgg attttgtgct aagaaaactg ctgtttgtgc aatatcttct acttgtccga
    12121 aacgctgcat cggaatttgc ttttccgctt gttccttgac tttgtctgct aaaacttccg
    12181 tcatatcagt tgtgatgaat cctggcgcaa tggcattaca tgtaatgcct ctagttgcag
    12241 cttctcgtgc cacagatttt gttaatccta ctacgccagc tttactagct gcataatttg
    12301 cttgaccaat atttccaatc aacccagata cactagagag attaatgatg gccccttctc
    12361 gctgcttcat catttttttc aatacgtgct gcgtcatatt gaaggttcca atcaaattga
    12421 tatccagaca ttttttgaaa tcttctgcat ccattcgcat cacaagtttg tcatttgtga
    12481 ttccggcatt gtttactaac acatggatcg atccaagctt ttcttctgct tctttgatca
    12541 tttgtcccgc tttctcataa tcagagatgt caccggaaac tcctacgcat tttacaccga
    12601 atgcttcgat ttcttctatc ttttcttttg gaatctctcc gcgaccattc aaaatgatat
    12661 ttgctccagc ttttgcaaaa gctaaggcca tcgctttgcc gattcctctt gtactacctg
    12721 tgataaatac attttttcct tttacatcca taacgttcct ccttatagta ttgctattgt
    12781 ttcatcaaat gtctgcttat tctcaacacg cagaactggt attgttttat cgattttttt
    12841 cataaatccg cttaatactt ttccggggcc gacttcgatg acttgttcga tgccgattgt
    12901 ttttaactta tggatactct catagaaacg aacaggttgc atgacttgct gttcaagtag
    12961 tcccgcgatt gtttctttct tcattatttc cgttgttgtg ttgcttatga taggaaaaga
    13021 aggttcagag aattgaatct gacttaggtc cttcgccagt ttttttgctg ccggttctaa
    13081 aatagctgta tgaaaaggcc cgctgacatt tagagggatc attcttttga accctttttc
    13141 ttttaatagt aaaacagctt cgtctactgc tttttcttcg ccaccaatca cgatctgctg
    13201 aggtgtatta taatttgcag gagatacgat gccgtacttg ctagcttcat gacagctttc
    13261 ctcgatcgtt tcaatcggcg cattcatcac tgcaaccatt ttcccgcttc cagctggggt
    13321 agcttctgtc atataagcgc ctctttttgc tactaacgca actgcttcag tgaaggatag
    13381 cgcattacta gctactaatg ccgaatattc tcctaaactc agtcctaaag ctgcatctgg
    13441 gatgattcca tgctccttca ataagcgata atacgcgatg ctgactgtta gaatagctgg
    13501 ctgtgtatat tgcgtctgat ccaaacgttc attttctgta aagcaaagtt cagccatatc
    13561 gtagccgaga atctcacttg cttcatcaaa tgtctgcttt acaacggctt cttcatataa
    13621 atcttttccc ataccttgat attgtgcacc ttggccacta aataagaatg ctgttttcat
    13681 gtttttctcc aatatatcca gaatatatgt ttgattcttc ctattcaaaa aggccgagac
    13741 attcctattt caggaaatgt cccagccccc aacctaattt taatgattat gcagtttgtt
    13801 tttctacgta agtcactaaa tcttgaaccg ttttgattcc gtcttctgtt tcgattttca
    13861 cgtcaaattc atcttcaatt tcattgatga tttggaataa atccaagctg tcagcttcta
    13921 agtcttcttg gatgtcagta gtcaatttga tttcttcagt ttcttttcct aattcttctg
    13981 cgatgatttc ttgaattttt tcgaatacca taataatata ttcctccgat attgtttgct
    14041 ttttattttt ataatttaac agtctacaac tccatgagaa tacttcccca tgttaaacct
    14101 ccgccatagc ctgtaaatat tactttctgc tgttttccta aggtgatctt tccactagaa
    14161 actgcttcat ccaataagat agggatgctt gcagcagatg tattcccata tttatccata
    14221 ttggtcagga acttttcttg cggcatcttt acttttcggg caattttctc aataattctg
    14281 aggtttgcct gatgcaaaag cagataatca actggtttgt ctttcatgac ttcttggata
    14341 ttgtttgcaa cgtctcgtac ggcaaaatca aaaatatctc tgccagtcat ttgcaaataa
    14401 tacgaacctt ctgaatcttc tgaatagaac ggatttttat tttgctggta gccagcggtc
    14461 aatgagctac ttctggtacc gtcagctgct aatttttctt tcagcaattt tttctgtgga
    14521 ttcgcttcta agagaatccc acctgctcca tcgccaaaca acacagcagt cgtacgatca
    14581 gaccaatcga ttagcttaga tagtgtttca ccaccaatca ccagtccctt agttgagccg
    14641 cagcggatca ttttttcagc catgcttaac gcataaacaa atcctgagca cgcagcactg
    14701 atatcaaaag cgatagcatg agttgctccc aatttccctt gtacttgtgc tgctacagat
    14761 ggtgtcgtgt aatcgggcga cattgttgct actaaaataa aatccaaatc tttagggttt
    14821 agatttttct gttttatcaa tttttccgcc actaaaatgc ataaatcaga agtctcctga
    14881 tttgttacac atcttcgttc acgaataccc gtccttgacg aaatccattc atcagaggta
    14941 tccatcatct tggaaagatc attattagta ataatctttt caggaaggac gctggctgtt
    15001 gcagtaatca ttccaaattg ttccattaaa tatcctcact tatagtcttg caagaagtcg
    15061 tgcaagtttt tcagtgcatc cagcaaggca tgctgttcct ccgtagccat tccgtctaag
    15121 atgttcttga ccatttcacg atgaaaatgc tgatggaccc gaaatagtaa ctttcctttt
    15181 ttcgttagtc ccaactttac aacgcggcgg tcatcttcac tgcggatacg ttcgacatag
    15241 ccttttttta ctaacttgtt aatagcagtg gtcaaggtcc caacagtaat tgatagttct
    15301 ttggctactt ctgaagttgt ttttttcttg tacatcccga ttgcctcgat cgtgtgcatc
    15361 tctgtgattg acagatcttt gaattgagat tttttcagct cagactcttc gattgtcaaa
    15421 atatcattga agacactaac aagataatca tttactgttt ttaaatctgg ttccatgttc
    15481 caccgtccac taattagttt gattatcaaa tcttttgatg ttcaaactat atcttaactt
    15541 ttttcaaaat gcaaccttaa aagatcattt ttttactata tgagcttttt ttaagatttt
    15601 actcatttaa aacaactgat aaatcagcat ttataatttc ttttattaga aaaatcatct
    15661 atctcacata ttttgattat caaagtattt tctgtgaaca gaactataga attccctaaa
    15721 acttgtccat tatttcaaaa tttgttatag tctttttatt caaagcgtaa aaggagatcc
    15781 tatgttaaaa gaaaaaatga aaactctccc catgctgccg ggtgtctatc ttatgaaaga
    15841 ccgagatggt aaggtcatct atgttggtaa agcaaaacgg ctcaaaaata gagtaagcag
    15901 ctattttcat cagaataaac agcattcaaa aaaagtatta cgtatgattc atcatataac
    15961 agattttgat ttcgtcgtag tagacacgga attagatgct ttattgctcg agtgtcaatt
    16021 gatccaacat taccgtcctt tttataa
//
LOCUS       DAZKLD010000063        15699 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.063, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000063 DAZKLD010000000
VERSION     DAZKLD010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15699)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15699)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15699
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.063"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..497)
                     /locus_tag="ACG8KR_001599"
     CDS             complement(<1..497)
                     /locus_tag="ACG8KR_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEf1 family transposase"
                     /protein_id="HFQ0200967.1"
                     /translation="MNDFTTEIVQTLVTKGDLNELFRSHLEKAINTLLRTELTAFLDY
                     EKYDRTGFNSGNSRNGSYFRSIKTEYGELTLEIPRDRNGEFKQQTLPAYKRTNDTLET
                     TIIHLFEKGVTMSEIADLIEKMYGHHYTPQTMSNMTKVLTEEVNAFKSRALNDKYVAI
                     FMDAT"
     gene            complement(904..1701)
                     /locus_tag="ACG8KR_001600"
     CDS             complement(904..1701)
                     /locus_tag="ACG8KR_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HFQ0200968.1"
                     /translation="MMKKFFLFFLLLVSVGWPVATHADTATVNDEAGLFTTDQIQSLE
                     QQAEKLNEKIKGRVLIVTTTSNSEEPRDFADNYLRDAVGNDQNGSVLLLDMGQREIYI
                     STSGNMIDYLTDSRIDSILDDVYDQMTNAAYYNAAKAYLSKSATYVEDGVPGGHYRVD
                     EETGKITRYKVLTTVEIIIAVVLALILSLVFYFVTVSRYQLKSGIYKYPFREKSSIKL
                     TEKTDRLTNSFVTTRRIPKSPPPGSGGGGSTTHSSGGGTFGGGGRSF"
     gene            complement(1698..2882)
                     /locus_tag="ACG8KR_001601"
     CDS             complement(1698..2882)
                     /locus_tag="ACG8KR_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HFQ0200969.1"
                     /translation="MDVLTHKCPNCGGPLTFDPKDQKFHCEYCLNIYTEEEVSQYEQK
                     QKEARMAGEESKEEPSPTEDFTFTAQEQLADMNEAERKAVDEAGGFSDTADESADNTA
                     AIDLFLCPNCGAEIVTDATTAATYCYYCHNPVVLSGRLSGEFLPNKVLPFAVEKEEAI
                     NRFLAWTKKKWFVPKAFFNKDQIDKLTGVYFPYWATDAEVDGAMQANGTVIRIWRIGD
                     IEYTETKQFAVSRAGKLSFKELVKNALSKNTQQKMVEGVQPFPLDKAIDFKSQYLAGF
                     QAEKRDIEYEAIKSQIQSELKDYSEKLLKDTANGYTTLTGVRTSASITNEVNNYVLLP
                     VWLVTYRSNDSSKKVYYYAMNGQTGKVSGVLPISQKKLGLTALGIFAGLAILFLIGGY
                     LI"
     gene            complement(2907..4154)
                     /locus_tag="ACG8KR_001602"
     CDS             complement(2907..4154)
                     /locus_tag="ACG8KR_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFQ0200970.1"
                     /translation="MGLIKAATSMVGGGLADQWLEVIEPDNMSDTTVMTKGVKVRKDD
                     KRGSNRKGTEDVLTDGTVVHVYPNMMMLLVDGGKIIDYTAEEGYYTIKNDAAPSMFNG
                     TLKEAIAETFDRFKFGGVTPQKQQVFYINLQEIKGIKFGTSAPLNYFDNFYNAELFLR
                     AHGTYSIHVVDPILFYTNAIPKNKTQVEINDINEQYLAEFLTALQSAINQMSADGQRI
                     SYVPSKSLELSKYMDTALDDSWRELRGMEIVSVAVASISYTDDSVKLINMRNEGAMLG
                     DPSVREGYVQGSIARGMEAAGKNEAGAMTGFMGVGMGMNANGSYLSQAAQNNQEQIKQ
                     QAEKQNQQTAQGNADTWTCPVCGTENSGKFCSNCGAAKPVENVQPKLQMRCSECNEIV
                     DLSNGIPKFCPNCGKPFKGIPVN"
     gene            4369..6114
                     /gene="dnaX"
                     /locus_tag="ACG8KR_001603"
     CDS             4369..6114
                     /gene="dnaX"
                     /locus_tag="ACG8KR_001603"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HFQ0200971.1"
                     /translation="MAYQALYRVWRSQRFEDIVGQKAVTQTLKNAIVSHKTSHAYLFT
                     GPRGTGKTSAAKIFAKAINCPNSKDGEPCNECEMCRSITAGTQEDVIEIDAASNNGVE
                     EIRFIRDRANYAPTKAAYKVYIIDEVHMLSTGAFNALLKTLEEPKESVIFILATTEPH
                     KIPATIISRTQRFDFKRINTADIVEHLEFILKESTISYEPAALNVIARSAEGGMRDAL
                     SILDQAISFSEGTITLDDAMQVTGSLTDEMMDSYLSACVKKEVPEALETLNQILAAGK
                     ESRRFLEDMLQYCRDLLMYQQAPNLVEEQSSTIRSTFTELAQTVSADQLFSMIKILSD
                     TQNEIRFTNSAAIYLEVATVKLAQLPSASVPSADVAFADDQIVKQLQQEIKELRNEIK
                     ELRSNTGIENTKVQPVQQTNHKKQTSTYRVPTERVYKVLREATRQHLNQVKEVWEDLL
                     MSLSVTQRAMLKASEPVAAGPTGLVIAFDYEIVCQRAANDEELQLSVHNNLSRMIKDY
                     APDPVYITRESWPELRRNYLTQAQEEGTENSFASEDSEGEIELLPQEQEVPEETLVVK
                     AEELFGSEAVTVVDD"
     gene            6134..6448
                     /locus_tag="ACG8KR_001604"
     CDS             6134..6448
                     /locus_tag="ACG8KR_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HFQ0200972.1"
                     /translation="MMRGMGNMQGMMKQVQKMQKEMAEAQELLNKTEFTGKATNELVK
                     AVFTGDRRMKDIQINEEVVDPDDIEMLQDLVVMAVNDAIAQIDVETEKKLGKYAKGLP
                     GF"
     gene            6473..7069
                     /gene="recR"
                     /locus_tag="ACG8KR_001605"
     CDS             6473..7069
                     /gene="recR"
                     /locus_tag="ACG8KR_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HFQ0200973.1"
                     /translation="MQYPEPIAKLIDSYMKLPGIGQKTATRLAFYTIDMKDEVVNEFA
                     KSLLSVKRDLHFCSICGNITEEDPCEICRNPSRDRSVILVVEEPKDVMAMEKMREYHG
                     LYHVLHGVLSPMEGTGPEDINISSLLQRLHDADVKEVIIATNATTEGEATAMYLSRLI
                     KPAGIKVTRLAHGLSVGSDIEYADEVTLLKAVEGRREL"
     gene            7133..7777
                     /gene="tmk"
                     /locus_tag="ACG8KR_001606"
     CDS             7133..7777
                     /gene="tmk"
                     /locus_tag="ACG8KR_001606"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFQ0200974.1"
                     /translation="MNGLFITIEGPDGAGKTSILNELYPLLKEVAKTEIIQTREPGGI
                     PIAEEIRAVILDPKNDRMDERTEALLYAAARRQHLVEKVLPALAKGKIVLCDRFVDSS
                     LAYQGAGRRIGVTEIARLNEFATEGTTPDFTLYLDVDSDTGLRRIKKNRQNQIDRLDS
                     EGLEFHQRVRHAYLKLAEENPERIHKVDARKSFEEVLQTSYHTIIEQYPQFFEN"
     gene            7792..8121
                     /locus_tag="ACG8KR_001607"
     CDS             7792..8121
                     /locus_tag="ACG8KR_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HFQ0200975.1"
                     /translation="MKIILAIIQDKDSNRLSNELIDANIRATKLSSTGGFLKAGNSTF
                     IVGIEDDRVEEALEIIKKTCESRKQFVSTPVTLDISMDGGVPYPVEVEVGGATVFVLP
                     VEGFHQF"
     gene            8135..9073
                     /gene="holB"
                     /locus_tag="ACG8KR_001608"
     CDS             8135..9073
                     /gene="holB"
                     /locus_tag="ACG8KR_001608"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HFQ0200976.1"
                     /translation="MNQEINLAQMQPIVYQQLQRSFEHGRIAHAYLFEGEKGTGKHEV
                     GIWLAQHLFCTQMKDNLPCGKCNNCQRIEKKEHPDVLVIEPEGQTIKVDQIRRLQTEF
                     SRSGYESRKKVFLIKEAEKMNSSAANSLLKFLEEPPGDFLAVLETDAVGRILPTIQSR
                     CQVLHFQELSKEALIHRLQEKQIPLEKAKLLAFLTNSLGKAVEISQDEWFNDAKDLIY
                     QWFVYLQKQDTQAFIYVQKKLVKSFKEKTQQFTGLSVLMFYYQEAQRQAVANGEYGKV
                     KQINQIIERILIAEQKLRSNVSFQAVAEQFVLHTIF"
     gene            9109..9932
                     /locus_tag="ACG8KR_001609"
                     /pseudo
     CDS             9109..9932
                     /locus_tag="ACG8KR_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332104.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
     gene            9925..10272
                     /locus_tag="ACG8KR_001610"
     CDS             9925..10272
                     /locus_tag="ACG8KR_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HFQ0200977.1"
                     /translation="MDKRSLYDGLNQLESELRNTLTELVEMKHALHEIVEKNTTLEME
                     NQRLREHLRELNQVAQAPAENVKQELSKSRMNLEKIYEEGFHVCYDLYGSRRDNDEPC
                     AFCLEVIYRESGR"
     gene            10341..11213
                     /gene="rsmI"
                     /locus_tag="ACG8KR_001611"
     CDS             10341..11213
                     /gene="rsmI"
                     /locus_tag="ACG8KR_001611"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HFQ0200978.1"
                     /translation="MYNQKSFKGNPVYGDLYLVPTPIGNLEDMSYRSVRMLQESDLIA
                     SEDTRNTQKLLNHFEIHTPQKSLHEHNYKERVPQLIALLKEGKTIAQVSDAGMPSISD
                     PGHELVLACIKEGISVISIPGPTAGMTALIASGLVPQPFLFYGFLPRKKKEQKETLKK
                     LKEQTVTLIFYESPYRITATLTNFLEIFGNRQVVLCRELTKVHEEYLRGSTEELLAYI
                     EEHPVKGECCLLVEGNLFEMPVTEVDEELGTLKEQVEAKIASGEKPNAAIKAVALKNG
                     LKKQEVYRQYHELD"
     gene            11322..12443
                     /gene="dinB"
                     /locus_tag="ACG8KR_001612"
     CDS             11322..12443
                     /gene="dinB"
                     /locus_tag="ACG8KR_001612"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HFQ0200979.1"
                     /translation="MMELQFPLHNDTSRKIIHIDMDAFFASVEERDHPEFRGHPLVIA
                     RHPSDTGGRGVVTTANYEARKFGIHSAMSAQKAYELCPQAIFKPGDHHKYSDISKQVR
                     EVFYRYTDIVEPVSIDEAYLDVTENKINCKSAIKIARMIQADIWESVQLTCSAGVSYN
                     KFLAKLASDYQKPRGITVVAPQDAVPFLKALPIEKFHGVGKKTVPRMHELGIYTGKDL
                     YECTEMMLIRNFGKMGYSLYRKVRGIHDSPVNVTRERKSVGKEHTYGQPLQTEEAVLT
                     QLRQLAEKVEEALRRVQKHGKTVVLKVRYTDYSTVTKRVTLPEYIYKKEALFYQASLI
                     WEEILGVEKGIRLLGITLTNLDPMTYENIVLPLWENQEI"
     gene            complement(12497..13099)
                     /gene="nrdG"
                     /locus_tag="ACG8KR_001613"
     CDS             complement(12497..13099)
                     /gene="nrdG"
                     /locus_tag="ACG8KR_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HFQ0200980.1"
                     /translation="MRNPKPKEWLAKDYSQNYIADYKPFNFVDGEGVRNSLYVSGCLF
                     ACEGCFNKAAQNFRYGKPFTPELEEQIIADLRNDYVQGLTLLGGEPFLNTDVCLQVVR
                     RIRKEFGTTKDIWSWTGYTFDELLQDSEDKLELLSQIDILVDGRFELSKRDLKLQFRG
                     SSNQRIIDVQKSLESNQVVIWEKCTDATETYEQIKKQDLI"
     gene            complement(13414..15603)
                     /gene="nrdD"
                     /locus_tag="ACG8KR_001614"
     CDS             complement(13414..15603)
                     /gene="nrdD"
                     /locus_tag="ACG8KR_001614"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HFQ0200981.1"
                     /translation="MKVNYEEKTVVTQQPETSQMKVIKRDGRHVDFDEQKINEALIKA
                     EKKIHGSLSPLTYEKIQMIADLVVQEIKSRFAENVKIYEIQNIVEHILLEKNEYELAE
                     EYINYRTKRDFARSKATDINFSIEKLINKDQTVVNENANKDSNVFNTQRDLTAGIVGK
                     SIGLKMLPPHIANAHQKGDIHYHDLDYHPYTPMTNCCLIDFKGMLNNGFKIGNAEVES
                     PKSIQTATAQISQIIANVASSQYGGCSADRTDELLAPFAELNYKKHLKDAEEWIDSPE
                     RQKEYAKAKTKKDIFDAMQSLEYEINTLFTSNGQTPFTSLGFGLGENWFEREIQKAIL
                     QIRINGLGSEKRTAIFPKLIFTLKKGVNLNPEDPNYDIKQLALECATKRMYPDILNYD
                     KIVELTGSFKVPMGCRSFLQGWKDENGQEVNVGRMNLGVVTLNLPRIAIESKGDQNKF
                     WQLLSDRLEIMKDALLYRVERCKEAIPANAPILYMYGAFGKRLSRTDSVNELFKNRRA
                     TVSLGYIGLYEVASAFFGGEWETNPEAKAFTLDIVKELKANADAWGDEYGYHFSVYST
                     PSESLTDRFCRLDTEKFGIIENITDKEYYTNSFHYDVRKNPTPFEKLEFEKDYPKYCS
                     GGFIHYCEYPMLQQNPKALEAVWDFAYDKIGYLGTNTPIDHCYQCGFEGDFKPTERGF
                     ECPECGNHDPKTCDVVKRTCGYLGNPQARPMVHGRHKEISSRVKHMK"
BASE COUNT         4840 a         2951 c         3270 g         4638 t
ORIGIN      
        1 taagtagcgt ccataaaaat agcgacatac ttatcattta aggctctgga tttaaaggca
       61 tttacttctt cagtcagaac tttagtcatg ttggacatgg tttgtggagt atagtgatga
      121 ccgtacattt tttcgatcaa atcagcaatt tcagacatcg taacaccttt ttcgaataaa
      181 tggataatag tggtttccaa tgtatcgttt gttcttttgt aggctggtaa agtttgttgt
      241 ttaaactcac cattacgatc tctaggtatt tccaatgtta attcaccata ttcggttttg
      301 attgatcgaa agtaagaacc gtttctcgaa ttacctgaat taaaaccagt gcgatcatat
      361 ttttcgtaat ctaaaaaagc cgttaattca gtccgtagga gtgtgtttat cgctttttct
      421 aagtgcgaac ggaataattc atttaaatcg cctttagtga ctagagtttg cacaatttct
      481 gtagtaaaat cattcatagg gaagtcctct tttctgtgaa ttggttgtcg ttaactttat
      541 tctacagaag ggacttcctt ttttgtatgg attttttcat ttacacaaaa tattttatac
      601 tctctgccgt ttaactctga gtcacaagga acaatttcaa aaaacagctt ttcatgtttg
      661 atgaatattg tgacttgtga ccgaggagtt ggtaggagaa caccgtttac caagaggttg
      721 tgaaaaagct gttctgcatc aagtaataag aagagccaag taaaaacagt ctcttatgtt
      781 tttaacttgg ctggacttat caccgtagat gtaagctttt gaacgccgtt tatttgcaac
      841 aaaaggggct gtgacacgat ttttgtcaca gccctttttt tgcgaacatt catttagcaa
      901 tttttagaaa cttctgccgc caccgccaaa agtcccgcca ccgctagaat gggtagtact
      961 tccgccaccg ccgcttccag gaggtgggct tttggggatt cttcttgtcg tgacaaaaga
     1021 atttgtcaga cgatcggttt tttcagttaa tttgatactg gatttttctc ggaatggata
     1081 tttgtagatg cctgatttca attgataacg cgaaacagtc acaaaataaa agacaagact
     1141 aaggataaga gccaaaacaa cagcaatgat gatttctact gtcgtcaaaa ccttatagcg
     1201 tgtaattttg ccagtttctt cgtctacacg ataatgtcct cctggaactc cgtcttccac
     1261 atatgttgct gatttagaca ggtaagcttt tgcagcattg taataggcag catttgtcat
     1321 ctgatcataa acatcatcta agatcgaatc gattctgcta tcagtcaaat aatcgatcat
     1381 attaccagaa gtcgaaatgt agatttcacg ctgtcccata tctaatagca atactgagcc
     1441 attttgatca tttccgactg cgtctcttaa gtaattatca gcaaagtctc ttggttcttc
     1501 tgaattagat gtagtcgtta cgatcaagac tcgtcctttg attttttcat tcagcttttc
     1561 tgcctgttgt tccaaagatt ggatttgatc tgttgtgaat aacccagcct catcatttac
     1621 cgtagctgta tctgcatgag tagccaccgg ccagccaaca cttactagca aaagaaagaa
     1681 taaaaaaaat tttttcatca tatcaagtaa cctccgatca gaaagaggat cgctagccca
     1741 gcgaaaatcc caagagcggt cagtcccaat tttttctggc tgatcggcag gacaccgctg
     1801 acctttcctg tttgtccatt cattgcgtag tagtagactt ttttacttga gtcattgctt
     1861 cgataagtaa caagccatac cggcagtaat acgtaattat ttacttcgtt tgtgattgaa
     1921 gcagaagttc gcacaccggt caatgttgta tatccatttg cggtatcttt taacagcttc
     1981 tctgaatagt cttttaattc agattgaatc tgtgatttga ttgcctcata ttcgatatct
     2041 cttttttctg cttggaaccc ggctaaatac tggcttttaa aatcaattgc tttatctaac
     2101 gggaaaggtt gcacgccttc gaccatcttt tgttgcgtat tttttgataa ggcatttttt
     2161 actagttctt taaaagaaag cttgcctgct cttgaaacag caaattgctt cgtttctgtg
     2221 tattcgatgt cacctatccg ccatatgcgg ataactgtac cattcgcctg catggcacca
     2281 tctacttcag catctgtagc ccaataaggg aaatacacac ctgtcaactt atcgatttga
     2341 tccttattga aaaaagcttt gggaacaaac cattttttct tggtccaagc gagaaatctg
     2401 ttgattgctt cttctttctc gacagcaaaa ggaagtactt tatttggcaa gaattctccg
     2461 cttaaacgtc ccgatagtac gacgggatta tggcagtagt aacaataagt cgcagcagtt
     2521 gtcgcatcag tcacgatctc tgcaccacag ttgggacaaa gaaaaagatc tatcgctgcg
     2581 gtattatctg cagattcgtc cgctgtatct gaaaaaccgc ctgcctcgtc aaccgctttg
     2641 cgctcggctt cattcatatc ggcaagctgt tcttgggctg taaaagtaaa atcttcggta
     2701 ggtgaaggtt cttctttgct ttcttcgcca gccatacgcg cttctttttg cttttgctca
     2761 tattggctga cttcttcttc tgtatagata ttcaggcagt attcgcaatg aaatttttgg
     2821 tctttgggat caaaagtcaa cggaccgccg caattaggac atttatgtgt aagaacatcc
     2881 atcagctgca cacctttcta aataaattaa ttaacgggga ttcctttaaa cggtttacca
     2941 cagtttggac agaatttagg gatgccgttg gaaagatcca cgatctcatt gcattcactg
     3001 catcgcattt gtagcttagg ctgaacgttt tctacaggtt tggcagctcc gcagttggaa
     3061 cagaattttc cggaattttc tgtcccgcaa actggacatg tccaagtatc cgcatttcct
     3121 tgtgcagttt gttgattttg tttttctgct tgttgtttga tttgttcttg gttattttga
     3181 gctgcttgag aaagataaga accatttgca ttcattccca tacctacacc cataaagccg
     3241 gtcattgctc ctgcttcatt ttttccggct gcttccatac cacgtgcaat ggaaccttgc
     3301 acgtatcctt cacgtacgct aggatcacct agcatcgccc cttcgttacg catattgatc
     3361 agtttcacag aatcatcggt gtaagaaatg ctggctacag ccacagaaac aatctccatc
     3421 ccacgtaatt cacgccaaga atcatccaat gcagtatcca tgtatttgct taattccaaa
     3481 ctcttagatg gtacataaga aatccgttga ccatctgcag acatctgatt gattgctgat
     3541 tgtagcgctg ttaggaattc agctaaatac tgctcattga tatcattgat ttccacttgt
     3601 gttttgtttt tagggattgc gtttgtataa aataaaatcg ggtcaacgac atgaatagaa
     3661 taagtgccat gtgcccgtaa gaataattct gcgttataga aattgtcaaa gtagttcaac
     3721 ggcgcgcttg taccgaattt gattcccttt atttcttgga gattgatata aaaaacttgt
     3781 tgtttctgcg gtgtgacgcc gccaaatttg aatcggtcaa aagtttcagc aattgcttct
     3841 ttcagtgttc cattgaacat ggaaggagct gcgtcatttt taatggtgta ataaccttcc
     3901 tcagcggtat aatcgatgat ttttccacca tcgaccaaaa gcatcatcat gtttggatag
     3961 acatggacga ctgttccgtc tgttaaaaca tcttctgttc ctttacgatt ggagccacgt
     4021 ttgtcatctt tgcggacttt gacacctttt gtcatgaccg ttgtatcact catattgtct
     4081 ggttcgatga cttctagcca ttgatctgcc agaccgccgc caaccatact tgttgctgct
     4141 ttgataagac ccataaaaaa ttcctcctct gattcagcta gtgccaaaca attattgttt
     4201 atttattata ccataggaag atgtgaaact tctcggacta gagacggaat tgaaatggaa
     4261 aattgcgtat tgtttaaata cctgaaaaag ccgagaagga tagtagtttt tttacctaat
     4321 atcacgtata ataagagata gtgaattttt taaaaaaggg ggatccttat ggcatatcaa
     4381 gcactttatc gagtctggcg ttcgcagcgt ttcgaagaca tcgttggaca aaaagctgtg
     4441 acacagacac taaaaaatgc tattgtttcc cacaaaacct cacatgccta cttgtttaca
     4501 ggacccagag gaaccggaaa gacgagtgca gcaaagattt tcgcaaaagc aatcaactgt
     4561 ccgaacagta aagacggtga gccatgcaac gaatgcgaaa tgtgtcgatc aattactgct
     4621 ggtacgcaag aagatgtgat cgaaatcgat gctgccagca ataacggagt cgaagaaatc
     4681 cgatttatcc gtgatcgggc aaattatgcg cctacaaagg cagcgtacaa agtgtacatt
     4741 attgatgagg tacatatgct ttcaacagga gctttcaatg ccttgttgaa aacattggaa
     4801 gagccaaaag aaagtgtcat attcatctta gcaacgaccg aacctcacaa aataccagct
     4861 acgatcattt cacggactca gcgatttgat tttaaacgga tcaatacggc agatattgtc
     4921 gaacatcttg aatttatctt gaaagaatcg actatcagct atgaaccagc tgcattgaat
     4981 gtgattgccc gttctgcaga aggcgggatg cgggatgcat tgagtattct tgatcaggca
     5041 atctcgttta gtgaaggaac aattacttta gatgatgcca tgcaagtgac aggtagctta
     5101 actgacgaaa tgatggacag ttacttgagt gcttgcgtga aaaaagaagt accagaagca
     5161 ttggaaacat tgaaccaaat tctcgcagcc ggcaaagaat cacgtcgctt tctagaggat
     5221 atgttacaat actgccgtga cttgctgatg tatcagcaag cacctaacct agttgaggaa
     5281 caatcgagta cgatcagaag tacatttaca gaattggcac aaactgtttc agcggatcag
     5341 ctgttttcaa tgataaagat tttgagcgat actcaaaatg aaattcgttt taccaatagt
     5401 gccgctattt atttggaagt cgctacagtc aaacttgcac agctaccgtc tgcttctgtt
     5461 ccttcagcag atgtagcatt tgcagatgat cagatagtaa aacagctaca acaagagatt
     5521 aaggaactaa gaaacgaaat caaggaacta cgcagtaata caggaattga gaatacaaaa
     5581 gtacagccgg tacagcaaac aaatcataaa aaacagacaa gtacctatcg tgtaccgacg
     5641 gaacgagtat ataaagttct gcgggaagcg acaagacagc atctgaacca agtaaaagaa
     5701 gtatgggaag acttgctgat gagtctatct gtcactcagc gagcaatgct taaagcaagc
     5761 gaaccagttg cagctggacc tacaggacta gtgattgcgt ttgattatga gattgtttgt
     5821 caaagagcag caaatgacga agaattgcag ctttcggtac acaacaactt aagccgaatg
     5881 atcaaagatt atgcgcctga tcctgtttat attacaagag aaagctggcc agaacttcga
     5941 agaaattatt tgacgcaggc acaagaagag ggaacagaaa actcttttgc ttctgaagac
     6001 tcagaaggag aaatcgagtt gcttcctcaa gaacaagaag taccagaaga aacacttgta
     6061 gtaaaagcag aggaactgtt tggctccgaa gctgttacag tggtagatga ttaataaaaa
     6121 ggagtagata attatgatgc gtggaatggg caatatgcaa ggcatgatga aacaagtaca
     6181 aaaaatgcaa aaagaaatgg cagaagcaca agagctgcta aataaaacag agtttacagg
     6241 taaagccacc aatgaattgg tcaaagcggt ttttacaggc gatcgtcgca tgaaagatat
     6301 ccaaatcaat gaagaagtcg ttgatcctga tgatatcgag atgcttcagg atcttgtcgt
     6361 aatggcagtc aatgatgcga tcgcacaaat tgatgtggaa actgagaaaa aattagggaa
     6421 atacgcaaaa ggtttgccag gattttagtt ttaaactgaa ggagtaatga atatgcaata
     6481 tccagaacca attgcgaaac tgatcgacag ctacatgaaa cttccaggaa tcggtcagaa
     6541 aacagcgaca agactcgcct tttatacgat cgatatgaaa gatgaagtag taaatgaatt
     6601 tgctaaatct ttgttaagtg taaaacgcga tttgcatttt tgtagtatct gcggaaacat
     6661 cacagaagaa gatccctgcg aaatatgtag aaatccttca cgtgatcgaa gcgttatcct
     6721 agtggtcgaa gagccgaaag atgtaatggc aatggaaaaa atgcgagaat atcacggact
     6781 ttatcatgtt ctccatggtg tgttatcacc gatggaaggg acaggtccag aagacatcaa
     6841 tatttcttct ctgttgcagc ggctgcatga tgcagatgta aaagaagtga ttatcgcaac
     6901 aaatgcaaca acagagggag aagcaacagc tatgtatctc tcacgtctga tcaaacccgc
     6961 aggtatcaaa gtgactcgtc tagcacatgg tctgtctgta ggctctgata tcgagtatgc
     7021 agatgaagtt actttattga aagctgtcga aggaagacgt gagttataaa tttctaatta
     7081 agtgtttttt cagatacaat ataaaagcag cacaagagat gggggcaaca atgtgaacgg
     7141 actatttatt acaatcgagg ggcctgatgg tgcaggaaaa accagcatct tgaatgaact
     7201 ttaccctctt ttgaaagaag tagcaaaaac agaaatcatt caaacaagag aaccaggagg
     7261 cattccgatt gctgaagaga tccgagcagt gatccttgat ccaaaaaatg atcggatgga
     7321 tgaacgtacg gaagcactgc tttatgcagc agcaagaaga caacatctag tcgaaaaggt
     7381 tctgcctgct ctggcaaaag ggaaaatcgt tttatgcgat agatttgtcg acagttctct
     7441 tgcttatcaa ggtgcaggcc gacgcatcgg cgttaccgag attgctcggt tgaatgagtt
     7501 tgctacagaa ggtactacgc cggattttac cctttatctt gacgtagatt cagacaccgg
     7561 attaagaaga atcaagaaaa acagacaaaa tcaaatcgac cgtttagatt cggaaggttt
     7621 ggaattccac cagcgtgtac gtcatgctta cttaaagctg gcagaagaga atccggaaag
     7681 aatccacaaa gttgatgcaa gaaaaagctt tgaagaagtg ctgcaaacaa gttatcatac
     7741 aattattgaa cagtatccgc aattttttga aaactaggaa ggtgaacgtt tatgaaaatt
     7801 attttagcga ttatccaaga caaagacagc aaccgtttat caaatgaatt gattgatgca
     7861 aatatccgtg ccacaaaact atcatcgaca ggcggcttcc ttaaggctgg aaacagtacg
     7921 tttatcgtgg gtattgaaga tgaccgtgta gaagaagcat tggaaatcat caaaaaaacg
     7981 tgtgaatcaa gaaagcaatt tgtatctaca cctgttacac tggatatttc gatggatggg
     8041 ggcgtccctt atccagtaga agtagaagta ggcggtgcca cagtcttcgt cttacctgtt
     8101 gaaggattcc atcaatttta ggaggagctg agctatgaat caagaaataa acttggctca
     8161 aatgcaaccg atcgtgtatc agcagcttca acggagtttt gagcatgggc gtatcgctca
     8221 tgcttatctt tttgaagggg agaaaggaac aggaaaacat gaagtaggta tatggcttgc
     8281 acagcacctg ttctgtaccc aaatgaaaga caatctgcct tgtggaaaat gcaacaattg
     8341 tcagcggatc gaaaagaaag aacatccaga tgttttggtt attgaaccgg aaggacaaac
     8401 catcaaagtc gatcagatca gacgtttgca gacggaattc agtcgcagcg gttacgaatc
     8461 aagaaaaaaa gtttttttga tcaaagaagc tgaaaaaatg aatagcagtg cggcaaacag
     8521 tttgttgaaa ttcttggaag agccccctgg agatttttta gcggtgttag aaacagatgc
     8581 agtaggaaga attctcccta ctattcaatc tcgttgtcaa gttctgcatt ttcaagaact
     8641 ttcgaaagaa gcactgatcc atcgattgca agaaaagcag attccattgg aaaaagcaaa
     8701 acttttggct ttcttaacaa acagtttagg aaaagcagtt gaaatctctc aagatgaatg
     8761 gtttaatgat gctaaggact tgatttatca atggttcgtt tacttgcaaa agcaagatac
     8821 tcaagccttt atctatgtcc aaaaaaaatt ggtaaaatca tttaaagaaa aaacacaaca
     8881 gtttacagga ttatctgtct tgatgtttta ttatcaggaa gcacagcgtc aagcagtagc
     8941 aaacggcgaa tatggcaaag tgaaacagat aaaccagata attgaacgga tattgatcgc
     9001 agagcagaaa ctacgcagca atgtcagttt tcaggctgtt gcggaacagt tcgttttgca
     9061 tacgattttt tagcttgccg caaaggatga tgatcaagga ggatggaaat ggtagaagta
     9121 gtaggtgtcc gatatagaga tgcaggtcat atctattatt ttgcaccagg agactctgaa
     9181 tatgtttata gtgacaaggt attagtcgaa tctcagcaat ccttacatat tgctactgtt
     9241 gtccttccta aaaaagagat tgatcccaat gaccttcctg aagctatccg tccgatcttg
     9301 cataaagcgt cacaagctga attagaaaaa gacaaaaaaa caaaaaagat gcttatgatg
     9361 cattgaaaat cgcaaaagaa aaagtgcagt tgcgtgagtt ggaaatgaaa atggtcaaag
     9421 ccgaatacac atttgatcga aataaattga ttttttcttt cacagcggac gggcgaattg
     9481 actttagaga attggtaaaa gatctggccg ctgtatttcg tactcgtatt gagcttcgac
     9541 aaattggtgt ccgcgatgaa gcaaagataa taggcggaat tggtccatgt gggcgtccat
     9601 tatgctgttc ttctttttta ggcgatttcg tgcctgtatc aatcaaaatg gcaaaagatc
     9661 aaagtttatc tttgaatcca acaaaaattt ccggtttatg cggacgcttg atgtgctgtc
     9721 tgcaatatga gagcgaagaa tacgagacag cgaaaaaaga actccctgac tttggaaaag
     9781 taattgctac acctgatggt aaagggaaag tcgtagggtt gaatctgctt agccgtatcg
     9841 ttagtattcg gctgactgga cgggaaaatc ctgtcgaata tgaatgggat gaattgaaag
     9901 aatttatcca agtaggtgag atgaatggat aagagatcat tatacgacgg attgaatcaa
     9961 ttagaatcag agttgagaaa tacattaaca gaacttgttg aaatgaagca tgctttgcat
    10021 gagatcgtag aaaaaaatac aactttggag atggaaaatc aacgtctgcg ggaacacctg
    10081 cgggaattga accaagttgc acaagctcca gctgaaaatg tgaagcagga actttctaag
    10141 tctcgtatga atcttgaaaa aatttatgaa gaaggattcc atgtctgcta cgatttatat
    10201 ggatcaagaa gggacaatga cgagccttgt gcgttttgtc tagaagtgat ctatcgcgaa
    10261 agcggtcgat gaccaataac aagaggagac tggagcggag aaatactgct ccagtctttt
    10321 tattaaggag attgtgacag atgtataatc aaaaaagttt caagggaaat ccggtatatg
    10381 gggatttata tcttgtaccg acgccaattg gaaatttaga agatatgagc tacagaagtg
    10441 ttcgtatgct gcaagaatcc gatcttatcg caagtgagga tacgcgtaat acacaaaagt
    10501 tattgaatca ttttgaaatc catacacccc aaaaaagttt acatgaacat aattataaag
    10561 aacgtgtccc tcaattgatt gctttgttaa aagaaggaaa aacgattgct caagtcagtg
    10621 acgcaggaat gccttccatc agcgatcctg gacatgagct agttttagcc tgcataaaag
    10681 aagggatttc tgtgatctct atcccaggac ctacagctgg gatgacggct ttgattgctt
    10741 cagggctagt gcctcaacca tttttatttt atggcttcct tccacgtaag aaaaaagaac
    10801 agaaagaaac attgaaaaag ctaaaagaac agacagttac gctgattttt tatgagtcac
    10861 cttatcggat cactgctaca ttgacgaatt tcttagaaat ttttggaaat cgccaagtcg
    10921 ttttgtgtcg ggaattgaca aaggtccatg aagaatacct tagaggaagt acggaagagc
    10981 tactcgctta tatcgaagaa catccagtaa agggtgaatg ttgtctgctt gtagaaggga
    11041 atttgttcga aatgccagtg acggaagttg atgaagagtt gggaacgtta aaggaacaag
    11101 tagaagcaaa gatcgcttca ggcgaaaaac caaatgctgc tatcaaagca gtagcgctga
    11161 aaaatgggct gaaaaaacaa gaagtctacc gtcagtacca tgaattagac tgagatagta
    11221 taagttgtct ataatgaaag actaatgagg aaatcagcta ctaacttttc aaatctcttg
    11281 aacgcgaacg aatgttcttg tataatgaat aagaggtgag gatgatggag ctgcaatttc
    11341 ctttacataa tgatacatca agaaaaatca ttcatattga tatggatgct tttttcgctt
    11401 cggttgaaga acgagatcat cctgagtttc gcggacatcc attagtaatt gccagacacc
    11461 ccagtgacac tggtgggcgt ggagtagtga ctacagcaaa ttatgaagca agaaaattcg
    11521 gaattcattc agcaatgagt gcacaaaaag cgtatgagct ttgtccgcaa gccatcttta
    11581 agcctggcga ccatcataaa tacagcgata tttccaaaca agtacgggaa gtattctatc
    11641 gttatacaga tattgtggaa ccagttagta tcgatgaagc gtatcttgat gtgaccgaaa
    11701 ataaaatcaa ttgcaagtca gccatcaaaa tcgcccgaat gatccaagca gatatttggg
    11761 agagtgtcca gctaacatgt tctgcgggag taagttacaa taagtttttg gccaaattag
    11821 catcagacta tcaaaaacct agaggaatca cggtagttgc ccctcaagac gctgtgccat
    11881 ttttaaaagc tttgccaatt gaaaaatttc atggggtagg gaagaaaact gtacctagaa
    11941 tgcatgagct agggatttat acaggaaaag acctatatga atgcacagaa atgatgttga
    12001 ttcgaaattt tggaaaaatg ggctattctc tttatcgtaa agtcagagga atccatgatt
    12061 ctccagtaaa cgttacaaga gagcgaaaat ctgtgggaaa agagcatact tatgggcagc
    12121 cgttgcaaac ggaagaagca gtgctgactc agttgcgcca actagctgaa aaagtagaag
    12181 aagcactgag aagagtccaa aaacatggga aaacagtagt tttgaaggtc aggtacacgg
    12241 actattctac tgtgaccaaa agagtgacat tgccagaata tatctataaa aaagaagcac
    12301 tcttttatca agcaagcctt atatgggagg aaatcttggg agtagaaaaa ggaatccgat
    12361 tgttagggat tactttgacg aatctggacc cgatgactta tgaaaacatc gtccttccat
    12421 tatgggaaaa tcaagaaata taaaaacagc tttgagagtt gaaaccggaa atccgttttc
    12481 tctcccaaag ctgtttttat atcaagtctt gctttttgat ttgttcatag gtttctgttg
    12541 catccgtaca tttttcccaa atcacaactt gattggattc taaagatttt tggacgtcaa
    12601 tgatccgctg gttgctactt ccgcgaaatt gtagttttag gtcacgtttg cttaattcaa
    12661 agcgtccgtc aacaaggata tcgatttgcg aaaggagttc taatttatct tctgaatctt
    12721 gtagcaattc atcaaaggta tagcctgtcc atgaccagat atcttttgtc gtaccgaact
    12781 cttttcggat acgtcgtacg acttgcagac aaacatctgt attcaaaaaa ggttcgccac
    12841 caagtaaagt taatccttgc acgtaatcgt tgcgcaagtc agcgatgatt tgttcttcta
    12901 attcaggtgt gaatggtttg ccatatcgga aattttgtgc agctttgttg aagcagcctt
    12961 cgcaggcaaa taagcagcca ctaacgtaaa gactgttgcg gacaccttca ccatcgacaa
    13021 aattaaacgg tttgtagtca gcaatatagt tttggctgta gtcttttgct aaccattctt
    13081 ttggttttgg atttctcata aaaaaacttc tttcatttaa ggttcaaaga tatagctatt
    13141 gaagataaaa tctactcgac cacataaata agaacaaact gacctaaatc cattgttcaa
    13201 gaaagcttgt tcttattttg atagagggcg ttcaaagata caaagccatc gaaagagggt
    13261 ggtgaaaaaa gacttgtcat agcctcttat tctgaataaa cggtgttcaa aagcttgcat
    13321 ctgcagtaat aagcccaact aaacgaaaaa tatgaggaac tatttttgtt tagctattct
    13381 tattacttga tgcaggacag ctttttcaca acctcatttc atgtgtttca cacgtgagga
    13441 aatttcttta tggcggccat gtaccatcgg acgagcttgc ggatttccta aatagccaca
    13501 agtacgtttg actacatcac atgtttttgg atcatgattt ccacattctg gacattcgaa
    13561 ccctcgttct gtcggtttga aatcaccttc gaaaccacat tgatagcaat gatcgattgg
    13621 cgtattcgta ccaagatacc cgattttgtc atatgcaaaa tcccatacag cttctaaagc
    13681 tttcggattt tgttggagca tagggtattc acagtagtgg atgaatccac ctgaacaata
    13741 ttttggataa tccttctcaa attccaattt ttcaaatggc gttgggtttt tacgcacatc
    13801 atagtggaaa ctgtttgtat aatattcttt atctgtgata ttttcgatga tgccgaattt
    13861 ttctgtatcc aaacggcaga aacggtcagt tagactttca cttggagttg aatagacact
    13921 gaaatgataa ccatattcat ccccccatgc atctgcatta gcttttaatt ctttgacgat
    13981 atcaagtgtg aaagcttttg cttctgggtt tgtttcccat tcgccaccga agaaagctga
    14041 agcaacttca tacaatccaa tgtagcctaa cgatacagta gctcgacggt ttttgaataa
    14101 ttcattgaca gaatcggtac gtgaaagacg tttcccgaaa gcaccataca tatataaaat
    14161 tggcgcattt gctggaatgg cttctttaca acgttctaca cgatagagaa gcgcatcttt
    14221 catgatttct aagcggtcag acagtaactg ccagaattta ttctgatctc cttttgattc
    14281 gatcgcgatt cttggcaaat tcaatgtaac aacacctaaa ttcattcgtc cgacatttac
    14341 ttcttgacca ttttcgtctt tccacccttg caagaatgaa cgacagccca taggtacttt
    14401 gaaacttcca gttaattcaa cgattttatc gtaattcaga atatcaggat acattctttt
    14461 cgttgcacat tccaatgcta gttgtttgat gtcgtaatta ggatcttcag ggtttaagtt
    14521 cacacctttt ttcaaagtga agatcagttt agggaagata gctgtccgct tttcactccc
    14581 taatccattg attcgaatct gtaaaattgc tttttgaatt tctctttcaa accagttttc
    14641 tcctagtcca aatcctaatg aagtgaaagg agtttgtcca tttgaagtaa ataaggtatt
    14701 gatttcatat tccaaacttt gcatagcatc gaagatatct tttttggttt ttgcttttgc
    14761 atattctttt tggcgttctg gcgaatcgat ccattcttcc gcgtctttta gatgtttttt
    14821 atagttcaat tctgcaaaag gagcaagcaa ttcatctgtt cggtcggcag aacagccacc
    14881 atattgactt gacgctacat tggcaataat ctgtgagatt tgtgctgtgg cggtctggat
    14941 agattttggc gattcgactt cggcattgcc gattttgaac ccattgttca gcatgccttt
    15001 gaaatcaatc aagcagcaat tcgtcatcgg tgtataagga tgataatcca aatcatggta
    15061 atggatatcg cctttttgat gcgcatttgc tatatgtggt ggcaacattt tcagtccgat
    15121 tgattttcca acgatcccgg ctgtcagatc tcgctgtgta ttgaaaacat tactgtcttt
    15181 atttgcgttt tcgttcacga cagtttggtc cttgttgatt agcttttcaa tcgaaaagtt
    15241 gatatctgtt gctttactgc gagcaaaatc tcgtttggta cgataattga tgtactcttc
    15301 tgctaattca tattcgtttt tttctagtaa tatatgttcc acgatgtttt gaatctcata
    15361 gattttgaca ttttcagcaa accggctttt gatttcttgg acaaccaagt ctgcaatcat
    15421 ttgaattttt tcataagtaa gtggtgataa tgagccgtga attttctttt ctgctttgat
    15481 cagtgcttca ttgatctttt gttcatcgaa atcaacatgg cggccatcgc gtttgatgac
    15541 tttcatctgg gatgtctctg gctgctgagt aacgacagtt ttttcttcat aatttacttt
    15601 cattatcttc aactcctgct aaattgttct tgtctatcat aaaacaaaat cggggaagga
    15661 gcaactaaat atcggctata tatagtattc aatgtgctg
//
LOCUS       DAZKLD010000064        15296 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.064, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000064 DAZKLD010000000
VERSION     DAZKLD010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15296)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15296)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15296
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.064"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            333..947
                     /gene="gmk"
                     /locus_tag="ACG8KR_001615"
     CDS             333..947
                     /gene="gmk"
                     /locus_tag="ACG8KR_001615"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HFQ0200982.1"
                     /translation="MSERGLLIVLSGPSGVGKGTVRKAIFESEDNDFQYSISMTTRKM
                     REGEVEGVDYFFRSKEEFESMIEAGEMLEYAEYVGNYYGTPLSYINKTLDEGKDVFLE
                     IEVQGAKQVKEKVPDGVFIFLTPPDLAELKSRIVGRGTDADEVIEERMKVAKEEIEMM
                     ALYDYAVVNDEVPLAVERIKNIIASEHFRVDRVIGKYIKMLKEM"
     gene            952..1257
                     /gene="rpoZ"
                     /locus_tag="ACG8KR_001616"
     CDS             952..1257
                     /gene="rpoZ"
                     /locus_tag="ACG8KR_001616"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFQ0200983.1"
                     /translation="MMLKPSIDSLLDRVNSKYSLVILASKRAHELDASAQPTLDSFDS
                     VKSVGQALEEIDAGNVVNDPHPELKRERLKMEEEERQAQKEREQHELESRIREEQKM"
     gene            1510..3918
                     /gene="priA"
                     /locus_tag="ACG8KR_001617"
     CDS             1510..3918
                     /gene="priA"
                     /locus_tag="ACG8KR_001617"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HFQ0200984.1"
                     /translation="MTLTAEVIVDVPTMQTDQPFTYLVPSEMETALQVGMRVEVPFGN
                     GNRHVQGFVMAIQKSEESANPSLKAVIRLLDLAPVVNEELLSLADYMKKITYAFKITC
                     LQTMLPSVMKAEYDKLIYPLADTPEVQVLFDQREVISWKEAEEEGSLSQLIRWRQEQL
                     VDIKYEVHTRNKVKTIRLVRSLLTEKQIEEEWAKLRQNAKKQKELLLCLSELSQEEPI
                     AYFKNKGISTAVLNQGKEKGWLEFIESERYRDPYKDRVFDQTTALELNAEQKNAVEQI
                     ITAGQQQKDQVFLLEGITGSGKTEVYLQAIADVLSENKTAIMLVPEIALTPQMVERFK
                     GRFGESVAVLHSGLSQGEKYDEWRKIEREEAQVVVGARSAIFAPLKNIGLIIIDEEHE
                     SSYKQDETPRYHARDLAIWRSKYHHCPIVLGSATPSLESRARAQKGVYQLLQLNHRAK
                     AAAQLPAIEIVDMREEFQNHRTSTFSANLQEKIQNRLDKKEQTVLLLNRRGYSSFVMC
                     RDCGFVLPCPNCDISLTLHMDTRSMRCHYCGHEEPIPNRCPNCGGNKIRYYGTGTQKV
                     EEELRELYPQARILRMDVDTTRRKGAHEQILQKFGAKEADILLGTQMIAKGLDFPEVT
                     LVGVLNADTSLNLPDFRSSEHTFQLLTQVSGRAGRAEKAGEVVIQTFNPQHYAIELAK
                     KQNYEQFYQQEMHVRHRGGYPPYYFTVKITASHPEEQVAAKKIFQIANQLKEVLSPQS
                     LLLGPTPSMILRVKNRYYYQLIIKYKHEPNLPHVLDEILNGSQKEQRQGLFVAIDNEP
                     LNFI"
     gene            3933..4424
                     /gene="def"
                     /locus_tag="ACG8KR_001618"
     CDS             3933..4424
                     /gene="def"
                     /locus_tag="ACG8KR_001618"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFQ0200985.1"
                     /translation="MRYPILIHPNDKLKRTAQPIDVITDETIALLDNLYETMIANDGI
                     GIAAPQVGQNKRIAVIEVDEGEKFELINPEIIEAKGESLDVEGCLSIPHVYGTVKRAD
                     EVTVRYYDRDGEEIEVTAFGYLARAFQHEIDHLDGILFIEKMIQQIPEEELEEYMEEH
                     LDD"
     gene            4417..5355
                     /gene="fmt"
                     /locus_tag="ACG8KR_001619"
     CDS             4417..5355
                     /gene="fmt"
                     /locus_tag="ACG8KR_001619"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HFQ0200986.1"
                     /translation="MTKLVFMGTPAFSVPILEGLIESGYEIQAVVTQPDRPVGRKRVI
                     TPTPVKEAALKHGIRVLQPEKISGSPEMEEIIELVPDVIVTAAFGQFLPEKLLQVPKL
                     GAINVHASLLPKYRGGAPVHYAIMNGEKETGVTIMEMIKKMDAGGIYAQESIPITKQD
                     DVGTMFEKLSLLGRKLLLETLPNILDGQKPVPQDESEATFSPNITREQEAIDWHKTAE
                     EIDNQVRGMRPWPIAFTTYEGIRWKLLSVEVLAETTDKAPGTIIRKDKKNLWIACGKQ
                     TVLAINELQPAGKGKQAVHDFLNGSGQQVQIGQQVK"
     gene            5355..6713
                     /gene="rsmB"
                     /locus_tag="ACG8KR_001620"
     CDS             5355..6713
                     /gene="rsmB"
                     /locus_tag="ACG8KR_001620"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HFQ0200987.1"
                     /translation="MKKIPKKMKKSVRYMALLTLERVNKGGAYSNLLLNEMIKQSELN
                     QKDVGLFTELIYGTISHQRLLEFYVAPLIAKAKKVDDWVKTLLYLSVYQLEFLDKVPA
                     HAILNEAVEIAKVKGNPGTGKFVNGVLRNYQRQGAPDLQGIADPIERLSVKISLPLWM
                     TKRLVDQIGYEETEKLGLSLYQPSHASARVDTRRLSRDQAITVLQEEEITAEKSQVSP
                     YGIVAKKGHLASSSLFHDGVMTIQDESSMLVAPSMQIEPEHHVLDACAAPGGKTTHIA
                     SFLDPSCGGEVVALDVHEHKVKLIEDNAARLGVSSAVYAQKMDAREVSKQFEAEQFDR
                     ILVDAPCSGLGLLRRKPDIRYKKQPDELANLPKIQLEILESCAPTLKSSGILTYSTCT
                     ILKEENQDVVSAFLERHPDFERIDVLTNPMLQSSIEEKMLTLYPHQFYTDGFFICCLR
                     KK"
     gene            6726..7466
                     /locus_tag="ACG8KR_001621"
     CDS             6726..7466
                     /locus_tag="ACG8KR_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HFQ0200988.1"
                     /translation="MQIEYQSDVGRRRNTNQDYASVFTSRSGLKLAVLADGMGGHRAG
                     DIASQMAVTNLGEAWEKQEAVDDEKIAQWFIQSIQEENMLIYQRGQEQPEYNGMGTTI
                     VAAALSDDWFTIANVGDSRAYLIREDKIMQLTEDHSLVNELVKSGEISEEMAVNHPRK
                     NILTRSVGMPGTVEVDVSTHLWQLKDRLLLCSDGLTNMLSEEEIGTIINQEGPLSHKV
                     TELIDRANEAGGADNITVLLIEYKEDSV"
     gene            7463..9532
                     /gene="pknB"
                     /locus_tag="ACG8KR_001622"
     CDS             7463..9532
                     /gene="pknB"
                     /locus_tag="ACG8KR_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HFQ0200989.1"
                     /translation="MIELGKKLNGRYQITGNIGSGGMANVFLAHDLILDRDVAVKVLR
                     FDFQNDQTAIRRFQREALAATELVHPNIVSVYDVGEEDNMQYLVMEYVKGMDLKRYIQ
                     THYPVPYETAVNIMQQILSAISLAHNHQIIHRDLKPQNVLIDNEGVVKITDFGIAIAL
                     SETSITQTNTMLGSVHYLSPEQARGSMATKQSDIYALGIILYEMLTGSVPFDGESAVT
                     IALKHFQDDLPSIKALDPNVPQALENVILRATAKEPADRYKSAEEMSDDLSTVLSPAR
                     ANEEKWQPHVMDNETKVITPLTEDAPMPDSFKSMPLPKDKPENETLPEEEEVLPDPKK
                     KGQKKWWLILLAILAVLGIGTAVFFASGGRGEVVVPDVSDLSEASARETLAKAKLEVA
                     TKTEEIADDKIEEGNVVKTDPAAGTTVKQKREVTLYISSGTKKIKLDDYSGKSYEDAE
                     ASLKELGFSADLIQKEEEFSSEVESGMIISQSPSEDTEVDPKTDTITFTVSKGPETVT
                     LSSHVGENYETVRNWLVTQGFNINNISATYDYSDTVPSGSIISQDPASGEVVAEETYI
                     NFVVSQGTEPKLNDISGYTKSEAQSYLASVGAEYIGHETSEYSNTVDKDKVIRTVPGA
                     GTTITKGMVVNVIYSKGPDPASSSSEKDSSDSSSSSSSSSSTSNSSKEESSSSTSSSA
                     STSESSK"
     gene            9697..10596
                     /gene="rsgA"
                     /locus_tag="ACG8KR_001623"
     CDS             9697..10596
                     /gene="rsgA"
                     /locus_tag="ACG8KR_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HFQ0200990.1"
                     /translation="MALIKGQIRKAISGFYYVYANGETYQTRGRGNFRNRKITPLVGD
                     QVIFESENQTDGYLLEILPRKNQLVRPPVTNVDLGIIVTSIVEPAFSYNLLDRFLVTL
                     EYESIEPIIFLTKTDLSDDPEKIEEIKQTYTKIGYPVIVPNHTGDTTELIRYFPERLS
                     VFMGQSGAGKSTLLNQISPELNLATGEISDALGRGRHTTRHVELIPIYGGLVADTPGF
                     SSIDFLTIEAGELPRQFPEFVEASVHCKFRECMHAKEPGCEVKHRVETGGIAQTRYDN
                     YLQFLQEIEKRKPMYAKKEGKRK"
     gene            10609..11259
                     /gene="rpe"
                     /locus_tag="ACG8KR_001624"
     CDS             10609..11259
                     /gene="rpe"
                     /locus_tag="ACG8KR_001624"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HFQ0200991.1"
                     /translation="MKIAPSILSADFANLQRDIELVEKGGADYIHVDVMDGQFVPNIT
                     LGPNIVQAIRPVTKLPLDVHLMIVDPENYIPAFAKAGADIITVHVEATPHIHRALQMT
                     KSLGVKAGVVINPGTPVSMIKHVLPLADQVLVMTVNPGFGGQSFIEETVEKITELADL
                     RRENNWTYAIEVDGGIVPETAKICKDAGADVFVAGSYIYGAENPVGQIERLKEALD"
     gene            11260..11898
                     /locus_tag="ACG8KR_001625"
     CDS             11260..11898
                     /locus_tag="ACG8KR_001625"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004788 - thiamine diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HFQ0200992.1"
                     /translation="MKILLAAGGNPQNWPQSLLSKYDAYVGIDRGALYLLQAGYPVDL
                     AVGDFDSLSNEEQKEVFAKAKEYTRSPAEKDDTDTQLGILKTFERYPQAEVTLIGATG
                     GRLDHLLANLWLGMEPRFKPFAHQLIIRDCQNYLSYYLPGEHIVRQIPDMKYLAYCCL
                     TPVSNLTLKGSKYVLENKEVEYPTSYASNEFLSDTAEFSFSDGMIAVIQSKD"
     gene            12053..12907
                     /gene="mreC"
                     /locus_tag="ACG8KR_001626"
     CDS             12053..12907
                     /gene="mreC"
                     /locus_tag="ACG8KR_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HFQ0200993.1"
                     /translation="MKKFNPNKNIIITLVIVIIVVTILSVTAAQRANEGKPNFFQSIV
                     NDSVAMVDKTISAPVRWVRNGVDSVHNLFSTYAENERLKEKIDSYDDVSQQNKNYERE
                     IEALKKELELNQTLTSYEKVTANVITRSPDSWQDMLIVDKGSKDGIEANMAVMSQKGL
                     IGRVIEVNTASSKIELLSSSNESSNHFPVRVSSANGEAFGLLKNYDEKLHALVVTQLT
                     GDTDIKEGDVVQTSGLGGNSPANLPIGTVIKTKPDSYGLDREVYVKPYAEMYDVSVVT
                     IVQRLAED"
     gene            12911..13420
                     /gene="mreD"
                     /locus_tag="ACG8KR_001627"
     CDS             12911..13420
                     /gene="mreD"
                     /locus_tag="ACG8KR_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HFQ0200994.1"
                     /translation="MLKKETMKYYLPIVLFFLMLIDGHLTRMLGEWSKGTYMSNAHFL
                     ILALLCCSMAFEKRYLLITTIVLGAIYDAYYIGVIGIYAVALPLIVWLMYVMKDVIHV
                     NIFTEFFSMIIFVTGYELFTMVVQLIFKLAVVNNTYFITRFLGPTLLLNMIIFVLFIF
                     PFKKLFSDE"
     gene            13675..15249
                     /locus_tag="ACG8KR_001628"
     CDS             13675..15249
                     /locus_tag="ACG8KR_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HFQ0200995.1"
                     /translation="MKKSLISAVMVCSMTLTAVASPIAAAADDFDSQIQQQDQKIADL
                     KNQQADAQSQIDALESQVSEINTQAQDLLAKQDTLRQESAQLVKDIADLQERIEKRED
                     TIQKQAREAQVSNTSSNYIDAVLNADSLADAIGRVQAMTTMVKANNDLMEQQKQDKKA
                     VEDKKAENDAKLKELAENQAALESQKGDLLSKQADLNVLKTSLAAEQATAEDKKADLN
                     RQKAEAEAEQARIREQQRLAEQARQQAAQEKAEKEAREQAEAEAQATQASSTAQSSAT
                     EESSATQSSMTEESSSATQSSATEESTTPESSTEESTAPESSATEESTTAPESSATEE
                     STTVPESSATEESTTVPESSTTEESTTPAPTTPSTDQSVDTGNGTGSSTPAPTPTPTP
                     EQPKPVTPAPAPSGSVNGAAIVAEAYKYIGTPYVWGGKDPSGFDCSGFTRYVYMQVTG
                     RDIGGWTVPQESAGTKISVSQAKAGDLLFWGSQGGTYHVAIALGGGQYIHAPQPGESV
                     KVGSVQWFAPDFAVSM"
BASE COUNT         5127 a         2872 c         3324 g         3973 t
ORIGIN      
        1 gtattaagcc aaatttatgc aaaaatgtga gaaactattt tcacaaaatt tttcttcata
       61 ctcgaaacta atcggcttct tcacgacctc ttatacacgg cgttcaaaag cttgcatctg
      121 cgataataac cccaaccaaa cgaaaaacat gagaagctgt ttatgtttgg ctgttcttat
      181 tacttgatgc aggacagctt ttttacgacc tcttggtaca gggtagcttt tttaccaatt
      241 tctagctata attaaagata accttgaaag ttgcagaaaa aaagtgcaaa ataaacgata
      301 agtacaaaaa gactagttga aaggacgtcg tcatgtcaga gcgtggatta ttaatcgtgt
      361 tgtctggtcc atccggtgta ggaaaaggca cagtacgaaa agcaattttt gagagtgagg
      421 ataatgattt tcagtattcg atctcaatga ctacccgtaa aatgcgtgaa ggggaggtcg
      481 aaggcgttga ttatttcttc cgaagcaaag aagagtttga atcaatgatc gaagctggag
      541 agatgctgga atacgcagaa tatgtcggga attattatgg aactcctttg tcatatatca
      601 acaagacatt ggatgaagga aaagatgttt tcttggagat tgaagtccaa ggagcaaaac
      661 aagtcaaaga aaaagtgccc gatggtgtat tcatcttttt gactccgcca gatttagcgg
      721 aattaaaatc acggatcgta ggccgaggaa cagatgcaga tgaagtaatc gaagaacgaa
      781 tgaaagttgc aaaagaagaa atcgaaatga tggctcttta cgactatgct gttgtaaatg
      841 atgaagtgcc gctagcagtt gaacgaatca aaaacattat tgccagtgaa cattttcgag
      901 tagatcgcgt aatcggtaaa tatattaaaa tgttaaagga gatgtagtct tatgatgtta
      961 aaaccttcaa ttgactcact attagatcgt gtaaattcaa aatattctct agtgatttta
     1021 gcaagtaaac gcgcgcatga attggatgca agtgcacagc ctactctaga ttcttttgat
     1081 tcagtaaaaa gcgtcggtca agctttagaa gaaatcgatg caggaaatgt agtcaatgat
     1141 ccacatcctg aattaaaacg tgaacgtttg aagatggaag aagaggaacg tcaagcacaa
     1201 aaagagcgtg aacaacacga attggaatca cgtatccgtg aagagcaaaa aatgtaataa
     1261 agaaaaatag agcgaacatc taaaaagaaa agcaaaccca gctgtttcct tttcaaacat
     1321 tcgccactta ttgtctggta gaaaaggctg tgtctgaact ttttgacaca gttctttttt
     1381 ttcttatctg ctgttaattt tttgttagga tttggcagaa gataagacaa atccatcgat
     1441 ctctagtaaa ataggcaagg aactttttgt cgcttgataa gaaaagacaa aaaaaggagg
     1501 aaacgaacca tgacattaac agctgaagtc attgtggacg tacctactat gcagaccgat
     1561 cagccattca cttatttggt tccctctgaa atggaaacag ctttgcaagt cgggatgcga
     1621 gtagaagtcc cttttggtaa tggcaatcgt catgtccaag gatttgttat ggctatccaa
     1681 aaatcagaag aatcagctaa tccatctttg aaggcagtta ttcgtttgct agaccttgct
     1741 ccggtggtca atgaagaatt actttcatta gctgattata tgaaaaaaat tacttacgca
     1801 ttcaaaatca cttgtctaca aacgatgctt ccgagtgtga tgaaagcaga atatgataaa
     1861 ttgatctatc ctttggcaga cacaccagaa gttcaggtat tgtttgatca gcgagaagtg
     1921 atttcctgga aggaagcaga agaagaaggc agcctttctc aattgattcg ctggcgccaa
     1981 gagcaattag tagatatcaa atatgaagtc catacacgaa ataaagtaaa aacgattcgt
     2041 ttggtccgtt ctttgttaac tgaaaagcaa atcgaagaag aatgggcaaa attacggcaa
     2101 aatgccaaga aacaaaaaga acttcttttg tgtttatctg aactgagcca agaagaacca
     2161 attgcttatt tcaaaaataa aggaatcagt actgctgtct tgaatcaagg aaaagaaaag
     2221 ggctggctgg agtttataga atcagaacgt tatcgcgacc cttataaaga ccgagtattt
     2281 gaccaaacga cggcattgga actgaatgca gaacaaaaaa atgctgtcga acaaatcatc
     2341 acagcaggtc agcagcaaaa agatcaggtc tttttgctgg agggaatcac tggcagcggg
     2401 aaaacagaag tatatcttca ggcaatcgct gatgttttgt cagaaaataa aacggctatt
     2461 atgttagtgc ctgaaatcgc attgactccg caaatggtcg agcgattcaa aggacgcttc
     2521 ggagaatcag ttgcggtctt acatagcgga ctttcccaag gtgaaaaata tgatgaatgg
     2581 cgcaagattg agcgggaaga agcgcaagta gtagtaggcg cacgatctgc tatttttgcc
     2641 ccgctgaaaa atatcgggct gatcattatc gatgaagaac atgaatcaag ttacaaacaa
     2701 gatgagacac cgcgttatca tgcgagagat ctagcaattt ggcgcagtaa atatcatcat
     2761 tgcccaatag ttctaggaag cgcgacgccc tctttagaat caagagcacg agctcaaaaa
     2821 ggcgtttatc agttattgca gttaaaccac cgagccaaag cagctgcaca gcttccagct
     2881 attgagatcg tagatatgcg tgaagagttt cagaatcatc ggacctctac tttttctgct
     2941 aatttgcagg aaaaaataca aaatcgatta gacaaaaaag aacaaactgt cctactgctc
     3001 aatcgtcgag gatattcgtc ttttgtgatg tgtagggact gtggatttgt tcttccatgt
     3061 cctaattgcg atatctctct tacactgcat atggatacac gttcaatgcg ctgccattat
     3121 tgcggacatg aagaacctat tcccaatcgc tgtcctaatt gtggcggcaa taaaatccgc
     3181 tactatggaa caggtactca aaaagtagaa gaggaactcc gagaacttta tccgcaagct
     3241 aggattttgc gtatggatgt agataccact cgaagaaaag gggcacacga acagatttta
     3301 caaaaattcg gtgcaaaaga agcagatata ttattaggga cacaaatgat tgctaaaggt
     3361 ttggactttc cagaagtgac attagttggg gtgttgaatg ctgatacctc cctgaattta
     3421 ccggattttc gttctagtga gcatacattc caattgctta cccaagtttc aggacgagca
     3481 gggcgagcgg aaaaagcagg cgaagtcgtg atccaaacat tcaatcctca acattacgcc
     3541 atagaattag ctaaaaaaca aaattatgag cagttttatc agcaggaaat gcatgtacgg
     3601 catcgtggag gctatcctcc ttattacttt actgtgaaaa taaccgccag ccatcctgaa
     3661 gaacaagtag cggcaaagaa aatctttcag attgccaatc agctaaaaga agtgttaagc
     3721 cctcaaagct tgctattagg gccaacgccc agcatgattt tgagagtgaa aaaccgatat
     3781 tattatcaac taatcatcaa atataaacat gaaccgaatc ttcctcatgt tttggatgaa
     3841 attttgaatg gaagccagaa agaacagcga caaggtcttt ttgtggcaat tgacaacgaa
     3901 ccattaaact ttatttaaga aagggccaaa ttatgcgcta tcctatattg atccacccta
     3961 atgataaatt aaaaagaact gctcagccaa tcgatgtaat cacggacgag acaatcgctt
     4021 tattagacaa tttatatgag acaatgattg cgaatgacgg aatagggatc gctgctcctc
     4081 aagtaggaca aaacaagaga attgctgtga tcgaagtaga tgaaggcgag aaatttgaac
     4141 tgattaaccc agaaatcatc gaagcaaaag gtgaaagcct tgacgtagaa ggttgtctca
     4201 gtattccgca tgtctatgga acggtcaagc gagcagatga agtgaccgtg cgttattatg
     4261 atcgagacgg agaagagata gaagtgacgg cttttggcta tttagcccgt gccttccagc
     4321 atgaaatcga tcacttagac ggtatcttgt tcattgagaa aatgatacag cagatcccgg
     4381 aagaagaact tgaagaatat atggaggaac atcttgatga ctaagctagt atttatgggc
     4441 acacctgctt tttctgtgcc gattcttgaa gggctgatag aatcaggtta cgagatccag
     4501 gcagtggtaa cacagcccga ccgtccagtt gggcggaaac gagtaattac gccgacaccg
     4561 gtaaaagaag cggctctgaa gcacggtatc cgtgtgctgc agccagagaa aatctccggt
     4621 tcgccagaaa tggaagaaat cattgaattg gtgcctgatg tgatcgtcac tgcggcattt
     4681 ggccaattct tgcctgaaaa gttattgcaa gtaccaaaat taggggcaat caatgtccat
     4741 gcttctttac ttcctaaata tcggggaggt gcgcctgttc attatgccat catgaatggg
     4801 gagaaagaaa ctggtgtaac gatcatggaa atgatcaaaa aaatggatgc aggcggaatc
     4861 tatgcgcaag aaagcatacc aatcactaaa caagatgatg tagggacgat gtttgaaaag
     4921 cttagccttt taggaagaaa gctattgcta gaaacattgc ccaatatttt ggatggacaa
     4981 aaaccagtac ctcaagacga gtcagaagca actttttcgc caaacatcac acgggaacaa
     5041 gaagcaatcg attggcataa aactgcagaa gaaattgaca accaagtacg cggaatgcgt
     5101 ccttggccaa ttgcattcac gacctatgag ggcatacgct ggaaattatt atcggttgaa
     5161 gtattggcag aaactacaga caaagcacct ggtacgatca tccggaaaga caaaaaaaat
     5221 ctatggattg cttgcggtaa acagaccgtc ttagccatca atgaattaca gccagctggt
     5281 aaaggaaaac aggccgtaca tgatttcctt aacggaagcg gtcagcaagt tcagattggt
     5341 cagcaggtga aataatgaaa aaaatcccta aaaaaatgaa aaaatcagta cgatatatgg
     5401 cattactgac attagagcga gtgaacaagg gtggcgccta ctcgaatctt ttgttgaatg
     5461 agatgatcaa acaaagcgag ctaaatcaaa aagatgtggg tttatttaca gaattgatct
     5521 atggtacgat tagccatcag cgactattgg aattttacgt tgcaccattg atcgctaaag
     5581 caaaaaaagt ggatgactgg gtcaagacgc ttctttatct ttctgtatac caattagaat
     5641 ttttagataa agtccctgca catgctattt taaacgaggc ggtcgaaatc gcaaaagtaa
     5701 aaggaaatcc tgggacaggg aaatttgtaa atggtgtctt gaggaattat cagcgtcaag
     5761 gagcaccaga tctgcaaggc attgctgatc ctatcgaaag actttctgtc aaaatcagcc
     5821 tgcctctatg gatgacgaaa cgactggtgg atcaaatcgg atacgaagaa actgaaaagc
     5881 tcggtttatc gctatatcag cctagccatg ctagtgcccg agtagataca cgccgacttt
     5941 ccagagatca ggccatcact gttttgcaag aagaagagat cacagctgaa aaaagccaag
     6001 tcagtccata cggtatagta gcgaaaaaag ggcatttagc ttctagctca ttgttccacg
     6061 atggcgtcat gacgatccaa gatgaaagtt cgatgcttgt agcgccgtct atgcaaatcg
     6121 agcctgaaca tcatgtctta gatgcctgtg cagcacccgg aggtaagacg acgcatattg
     6181 cttctttttt agatccgtca tgcggtggag aagtggtcgc tttagatgta cacgaacaca
     6241 aagtcaaact cattgaagat aatgcagcac gtctaggtgt ctcttctgct gtctacgctc
     6301 aaaagatgga tgctagagaa gtcagtaaac agttcgaagc agagcagttt gatcggattt
     6361 tagtagacgc gccttgttca ggtcttgggc tgcttcggcg aaaaccagat attcgttata
     6421 aaaaacagcc ggatgaatta gcgaatttac caaaaatcca attggaaatt ttagaaagct
     6481 gcgcaccaac tttaaagtct tccggtattc tgacttatag cacttgtacg atcttaaaag
     6541 aagaaaacca agacgttgtt tctgctttct tggaaagaca ccctgatttt gagaggattg
     6601 atgttttgac caatcctatg ctacaatcat caatcgaaga gaaaatgttg actttgtatc
     6661 cgcatcagtt ttacacagat ggatttttca tttgttgttt gcgcaaaaag taatgaggtg
     6721 aaaaaatgca gattgaatat caatcagatg taggaagacg acgtaacaca aatcaagact
     6781 atgccagtgt atttaccagc cgttcaggtc tcaagcttgc tgtattagca gacgggatgg
     6841 gtggtcatcg tgccggagac attgccagtc agatggcggt aacgaatcta ggcgaagctt
     6901 gggaaaaaca agaagctgtt gacgatgaaa aaatcgccca atggttcatt caatcgattc
     6961 aagaagaaaa tatgctgatt tatcagcgtg gacaagaaca gccggaatat aacggtatgg
     7021 gaacaacaat cgtagcggcc gctctgtcag atgactggtt cacgattgcc aatgttgggg
     7081 atagcagagc ttatttgatt cgtgaagata aaatcatgca attgacggag gatcattctt
     7141 tagtcaatga actagtcaaa tcaggagaaa tcagcgaaga aatggcagtg aaccatccaa
     7201 ggaaaaatat tttaacacgt tctgtcggca tgcctggaac agtcgaagta gatgtttcta
     7261 ctcatctttg gcaactaaaa gatcgactgc tgttatgttc agatggcttg acgaacatgc
     7321 tgtctgaaga agaaatagga acgatcatca atcaagaagg cccattgtct cataaagtaa
     7381 ctgagctgat tgatcgtgcc aatgaagcag gcggagcgga taatatcacc gttcttctta
     7441 tcgaatataa ggaggacagt gtatgatcga gttgggcaaa aaattaaatg gccgttatca
     7501 aatcactgga aatatcggta gcggtggtat ggcaaatgta tttttggcgc acgatttaat
     7561 cttggatcgt gatgtagcag ttaaagtctt acgatttgac tttcaaaatg accagactgc
     7621 gattcgacga tttcagagag aagctttagc agcaacagaa ctcgttcatc caaacattgt
     7681 tagtgtttac gatgttggtg aagaagacaa catgcagtat ctggtgatgg aatacgtcaa
     7741 aggaatggac ttgaaacgat atatccaaac acattatcct gttccttatg aaacagcagt
     7801 aaatattatg cagcagatcc tttctgctat ttctttagct cataaccatc aaatcatcca
     7861 ccgagatttg aaaccccaaa acgtactgat cgataatgaa ggtgttgtaa aaatcacgga
     7921 tttcgggatt gctatcgctt tatctgaaac atcgatcacg cagacgaata cgatgctggg
     7981 atctgttcat tatctgtccc ctgagcaggc cagaggaagt atggcaacga agcagtcaga
     8041 tatttatgct ttagggatca tcctttatga gatgttgacg ggtagcgtac cgtttgatgg
     8101 tgaatctgct gtaacaattg ctttaaaaca ttttcaagat gatcttccat ctataaaagc
     8161 acttgatcca aatgtgccac aagcactgga aaacgtgatt ttacgggcaa cggctaaaga
     8221 acctgcagac cgttataagt cagccgagga aatgtctgat gatctttcta cagtattatc
     8281 accagctcgt gcaaacgaag aaaaatggca gcctcatgtg atggataatg aaacaaaagt
     8341 tatcacaccc cttacggaag atgcaccaat gcctgattca ttcaaatcta tgcctctgcc
     8401 aaaagacaag ccagaaaacg aaaccttgcc agaggaggaa gaagttctgc ctgatcctaa
     8461 aaagaaagga cagaaaaaat ggtggctgat cctattagct attcttgctg ttttaggaat
     8521 cggaacagct gtattctttg cttctggcgg gcgaggagaa gttgttgttc ctgatgtttc
     8581 tgatctttct gaagcttctg ctagagaaac actcgcaaaa gcgaaattag aagtagcaac
     8641 aaaaacagaa gaaattgcag atgacaaaat cgaagaaggt aatgtagtga agacagaccc
     8701 ggcagcggga acgacagtca agcaaaaacg cgaagtcact ctgtatatta gtagcggcac
     8761 gaaaaaaatc aaattagatg attacagtgg taaaagttat gaagatgccg aagcaagttt
     8821 gaaagaatta gggttttctg ctgacttgat tcaaaaagaa gaggaattca gttcagaggt
     8881 agaaagcgga atgatcattt cgcaatctcc atcagaagat acagaagtcg atcctaaaac
     8941 agacacgatt actttcacag tcagcaaagg accagaaaca gtgactttga gtagtcatgt
     9001 aggagaaaac tacgaaactg ttcgtaattg gcttgttaca caaggattca atatcaataa
     9061 tattagtgct acttatgact atagcgacac tgtaccatca ggttcgatca tcagtcagga
     9121 tccagcctcg ggtgaagttg tagcagaaga aacttacatc aattttgtcg taagccaagg
     9181 aacagagcct aaattgaatg atatctccgg ctatacaaaa agcgaagcac aaagttattt
     9241 agctagtgtc ggtgcagaat atatcgggca tgagacttcc gaatattcaa acacggtgga
     9301 taaagataaa gtgattcgta cagtaccagg ggcaggaacg acaattacca aaggaatggt
     9361 tgtgaacgta atttactcta aaggaccaga tccagcgtct tctagttctg aaaaagactc
     9421 ttcagacagt tctagcagct caagtagctc cagtagcaca agcaactctt caaaagaaga
     9481 atcttccagc tccacaagtt catcagctag cacctcagaa agttcaaaat aagtgctgaa
     9541 taaaatatat cagaagggaa tgcgacattg atttgttgca ttctcttttt tgtgtttcta
     9601 tctcttttga tcaattttca agccatatac tttgagaagt ttgcatggaa taacacctac
     9661 tttcctcttc gtatggtaaa ataaacaaaa acggaggtgg cacttatcaa aggacaaatc
     9721 agaaaagcaa tcagcggttt ttactatgta tacgcaaacg gagagaccta tcaaacaaga
     9781 ggaagaggaa atttccggaa tcggaaaatc acgccgcttg tcggagatca ggtgatcttt
     9841 gagagtgaaa atcaaacaga cggctatctt ctggaaatct tgccgagaaa aaatcaatta
     9901 gttcgcccac cagtcacaaa tgttgatctt ggaatcattg tcacaagtat cgtagagcct
     9961 gcattttctt ataatctact tgaccgcttt ctagttacct tggaatacga gtcgatcgaa
    10021 ccgattattt ttttgacaaa gaccgacttg tcagacgacc cagaaaaaat cgaagagatc
    10081 aagcaaacct atactaaaat aggctatccg gtgattgtac ccaatcatac gggtgatacg
    10141 acagaactga tccgttactt cccagaacgg ctatcggtct ttatgggtca gtctggagca
    10201 ggaaaatcga cgctactgaa tcaaatatca ccggaactga atttagcaac aggagagatt
    10261 tctgatgcac ttggaagagg acggcataca acacgtcatg tggaactgat tcctatctat
    10321 ggcgggttag tagcagatac accaggattt agctcgattg actttttgac aattgaagca
    10381 ggagaactgc cgagacaatt tcctgaattt gtagaagctt ctgtccattg caaatttaga
    10441 gagtgcatgc atgctaaaga acctggctgc gaagtaaaac atagagtaga aacaggtggt
    10501 atcgcacaga cacgttacga caattatttg caattcttac aagaaatcga aaaaagaaaa
    10561 ccaatgtatg caaaaaaaga aggtaaacga aaataggagg aaacaagaat gaagatagca
    10621 ccatccattt tgagtgcaga ttttgccaat ttacaaagag acattgagtt agtggaaaaa
    10681 ggaggcgcag attacatcca cgtagatgta atggatggcc aatttgtccc aaatatcaca
    10741 ttagggccaa atattgttca ggcaatccgt cctgttacta aattaccgtt agacgtccat
    10801 ctgatgatcg ttgatccaga aaactacatt ccagcatttg caaaagctgg agcagatatc
    10861 attactgtcc acgtcgaagc aactcctcat attcatcggg cacttcaaat gacaaaaagc
    10921 ttaggagtta aagctggagt agttatcaat ccaggaacac cagtctccat gatcaaacat
    10981 gttttgcctt tggctgatca agtactagtc atgactgtca atcctgggtt cggcggacaa
    11041 agctttattg aagaaacagt agaaaaaatt acggaactcg ctgatctacg tagagagaac
    11101 aattggactt atgcaatcga agtagatggc ggaatcgttc cagaaacagc aaaaatctgt
    11161 aaagatgcag gggcggatgt cttcgttgca ggatcatata tctatggagc cgaaaatcca
    11221 gtcggacaaa tcgaacgatt aaaagaggca ttagactaaa tgaaaatttt attagctgct
    11281 ggaggtaatc cgcaaaactg gccgcagtct ctgctgtcca aatacgatgc ctatgtcggg
    11341 atagaccgtg gtgcgctgta cctgcttcaa gcaggctacc cagtagatct ggcagtgggc
    11401 gattttgatt cattgtcgaa cgaagagcag aaggaagtat ttgcaaaagc caaagagtat
    11461 acccgttccc ctgcagaaaa agacgataca gatacacagt taggtatctt aaaaacattt
    11521 gaacgttacc cgcaagcgga agtcactttg attggagcta cgggcggaag attggatcat
    11581 cttttagcca atctctggtt aggtatggaa cctcgcttca aaccctttgc ccatcagctt
    11641 attatcagag attgtcaaaa ttatttgtct tattatttgc ctggagagca tatagtaaga
    11701 cagataccag atatgaagta tttagcttat tgctgcttga ctcccgtctc gaatcttaca
    11761 ttaaaaggca gcaagtatgt attggaaaat aaagaagtag aatatccgac atcttatgca
    11821 agtaacgaat ttctttcaga taccgcagaa ttttcttttt cagatggtat gattgcagtt
    11881 attcaaagca aagattgatc cataaacaaa taaaaaaatt tgtctttcag gctaaaaatg
    11941 acagaatcta ttatttgcta aaaaaaagct taagaggagc aacttacctt ttgtttcacg
    12001 gcatttagtg ataaaattga acacaagagt ctaattaatt cgaggtgtct ttgtgaagaa
    12061 gttcaatcct aataaaaata ttattattac cttggttatt gttatcatcg tggtgacgat
    12121 actaagtgtg accgctgcgc agcgagcgaa tgaaggaaag cccaatttct tccaatccat
    12181 cgttaatgac agtgttgcaa tggttgataa aacaatctct gccccagtac ggtgggtacg
    12241 aaatggagta gattctgtcc ataatctttt ttcaacttat gccgaaaatg agcggctaaa
    12301 agaaaaaatc gatagctatg atgacgtttc acagcaaaac aagaattacg agagagaaat
    12361 cgaagcgttg aaaaaagaac ttgagttgaa tcagacgctg actagttacg aaaaagtaac
    12421 ggcaaatgtc attactcgtt cgcctgacag ttggcaggac atgctgatcg tagacaaggg
    12481 ttctaaagac ggtatcgaag caaatatggc agtcatgtcg caaaaaggat tgattggccg
    12541 agtgatcgag gtcaatacgg cttcgtctaa aatcgaatta ctgtcatcct ctaatgaaag
    12601 ctccaatcat tttccagtac gggtatcttc ggctaatggc gaagcgtttg gtttgcttaa
    12661 aaactatgat gaaaagctcc atgccttagt ggtgacccaa ttaactggtg atacggatat
    12721 caaagaaggg gatgttgtcc agacatccgg tcttggaggg aattctccag ctaacttgcc
    12781 gatcggtacg gttattaaaa cgaaaccaga tagttatggg ctggatcggg aagtttatgt
    12841 gaaaccttat gcagaaatgt atgacgtgtc agttgtgacg attgtccaac ggctagcgga
    12901 ggattagtcc atgttgaaga aagaaacaat gaagtactat ctgccaatcg ttttgttctt
    12961 tttgatgttg atagatggtc atttaacaag aatgctaggg gagtggtcga aaggcaccta
    13021 tatgtcaaat gcccactttc tgatattggc attattatgt tgcagtatgg cgtttgaaaa
    13081 acgttattta ctgattacca cgattgttct cggggctatc tatgacgctt actatattgg
    13141 cgttatcggt atctatgcag tagctctccc tttaattgta tggttgatgt atgtaatgaa
    13201 agacgttatc catgtcaaca tctttactga atttttcagt atgatcatct ttgtcacggg
    13261 ttatgaattg tttacgatgg tggtccagtt gatttttaaa ttagcagtag taaataacac
    13321 gtattttatt acaaggtttt taggacctac actgctgttg aacatgatta tatttgtatt
    13381 attcattttt ccctttaaga aattattcag tgatgaataa tttctttttt tatttttagg
    13441 tcgaaagcga aaaagaatcg ttgttatctt aagaaatgct agacttaacg cggctttttc
    13501 atgatcattt tctgaaacga acagttttgt catttttaaa agttgttttg aaacattttt
    13561 gtaacataac tattatatcg ttgacacact gctatggtac aatttttata tcgttaagaa
    13621 agacttaaat atatttagat atttattaaa ttaagccagt cggaggaatg aagagtgaaa
    13681 aagagtttaa tatcagcagt aatggtatgt tcaatgacct tgactgccgt agcatcaccg
    13741 atcgctgccg cggcggacga ttttgattct cagatacaac agcaagatca aaagattgca
    13801 gacttgaaaa atcaacaagc agatgcacaa tcacaaatcg atgccttaga aagccaagtt
    13861 tctgaaatca acacacaagc acaagaccta ttagcaaaac aagatacatt gcgtcaagaa
    13921 tctgcacaat tagtaaaaga cattgcagat ttacaagaac gtattgaaaa acgtgaagat
    13981 acaatccaaa aacaagcacg cgaagcacaa gtaagcaaca caagctcaaa ctacatcgat
    14041 gctgtattga atgctgattc attagcagat gctattggac gcgttcaagc aatgacaacg
    14101 atggtaaaag caaacaacga cttgatggaa caacaaaaac aagataaaaa agctgtagaa
    14161 gacaaaaaag ctgaaaacga tgctaaacta aaagaattag ctgaaaacca agctgctttg
    14221 gaatctcaaa aaggcgatct tttatctaaa caagctgatt tgaacgtact gaaaacttca
    14281 ttagctgctg aacaagcaac tgctgaagac aagaaagctg acttgaaccg tcaaaaagca
    14341 gaagctgaag cagagcaagc tcgtattcgt gaacaacaac gtctagcaga acaagctcgt
    14401 caacaagctg cacaagaaaa agctgaaaaa gaagcacgcg aacaagcaga ggctgaagca
    14461 caagctactc aagcttcatc tactgcacaa agctcagcaa cagaagaatc atcagctaca
    14521 caatcttcaa tgactgaaga atcatcatca gctacacaat cttcagcaac agaagaatca
    14581 acaacacctg aatcttcaac agaagaatca acagcgcctg aatcttcagc aacagaagaa
    14641 tcaacaacag cgcctgaatc ttcagcaaca gaagaatcaa caacagtgcc tgaatcttca
    14701 gcaacagaag aatcaacaac agtgcctgaa tcttcaacaa cagaagaatc aacaacacct
    14761 gcgcctacaa caccatcaac agatcaaagt gttgatacag gaaacggcac aggaagtagt
    14821 actccggctc caacgccaac accaacacct gaacaaccaa aacctgtaac acctgctcca
    14881 gctccttctg gaagtgtgaa tggtgcagca atcgtagcag aagcttacaa atatattggt
    14941 actccatatg tttggggcgg taaagatcca agtggctttg actgctcagg gttcacacgc
    15001 tatgtttaca tgcaagtaac tggtcgtgac atcggtggtt ggacagttcc tcaagaatca
    15061 gctggaacaa aaatttcagt ttctcaagca aaagctggag acttgttatt ctgggggtca
    15121 caaggcggaa cttaccacgt agcgattgct ttaggcggag gacaatatat ccatgctcct
    15181 caaccaggcg aaagcgtaaa agttggttca gttcaatggt ttgcccctga ctttgctgtc
    15241 agcatgtaag aatgattaat caaaaagagt tagcccattc tggactaact cttttt
//
LOCUS       DAZKLD010000065        15259 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.065, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000065 DAZKLD010000000
VERSION     DAZKLD010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15259)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15259)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15259
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.065"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            44..535
                     /locus_tag="ACG8KR_001629"
     CDS             44..535
                     /locus_tag="ACG8KR_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HFQ0200996.1"
                     /translation="MAKDASFDIVSEVNTEEVKNAIQQTTKELSNRFDFKDSTVEIKL
                     ENSNLVVLSDDDFKIEQIKDVLFGKLIKRNVPIKNIHFSESEHALGGKARQTADLVNG
                     IDRENAKKITTAIKNEKLKVKAQIQEDQIRVTGKNRDDLQAVIALLRKLDLPIELQFT
                     NYR"
     gene            complement(559..2934)
                     /locus_tag="ACG8KR_001630"
     CDS             complement(559..2934)
                     /locus_tag="ACG8KR_001630"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HFQ0200997.1"
                     /translation="MIKQSKISVRHLVEFILRRGSIDNRKKSNHTALEGARIHRKLQK
                     DAGENYEKEVFLKTTVQLDEYYLIVEGRADGIFKRDGIYYIDEIKTSEPRFEDLEPEQ
                     VELFFHQARVYAYIYSQEHDLQEINLQLTYFQTAEEVITRKVEHQTREQLETFFKKLT
                     EDYKEWLVFQENWRTVRNASLMALKFPHDEYRKGQRELAVAAYKTIRTKQKLFVEAPT
                     GTGKTISTLFPALKAVGEEEGEKIFYLTAKTITRQVAEDAMTALKDTGAEVKSVTLTA
                     KDKICFLDETTCNPDQCPYANGYYNRINEGLWDLLNHENQITREVIETYARKHTLCPF
                     ELSLDVSIWCDVIIGDYNYLFDPTVYLRRFFEDEKNEDYLFLIDEAHNLVNRSREMYS
                     AELSYEKTKRSKEAIPKEFKKLHRRFNKLLKEFDSIREIAKEDHWDYHHQKAPAESLV
                     KAGYQLSEKIKEWLAEFPEHPSQEQLLPYYFDLLHFLKVSEFYDDHYETTVEKTYRDL
                     IVKEFCIDPSLFLEQSLDKGKSSILFSASFSPLSYYQETLGGQKSLAYKLPSPFPEGN
                     QQVFIANYLETTYRKREHSLAKLVETIHAFADGKVGNYFVFFPSYQYLDLAVEAFRQQ
                     YPDSNVLIQETDMNEEERERFLTKFQTNPAETLIGFCVLGGIFSEGIDLRGDRLIGSM
                     IVGVGLPQMNHEQELIKSYYDEKEHLGFAYAYQLPGMNKVLQAGGRVIRGMADQGIVV
                     LADQRFSSFSYRRLFPQHWQTAREVRSVGELEEGIQRFWLSKEAVNQKNEE"
     gene            3260..3886
                     /locus_tag="ACG8KR_001631"
     CDS             3260..3886
                     /locus_tag="ACG8KR_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFQ0200998.1"
                     /translation="MHIERLKTGMIEENCYLVYNEEALLIIDPGEDAERIKQEIKKTN
                     QQPVAILLTHTHYDHIGAVEPLRAYYQIPVYVSPLEQKWLGDPILNLSGLGRHDDIAD
                     VIVSPAEYEFEMKTYTLGNMSFRVVPTPGHSIGSVSFIFDDFVVSGDALFRGSIGRTD
                     LYTGNLEQLLHSIRTQLFVLPDEFAVYPGHGDATTIEQEKRTNPFFNR"
     gene            3909..4613
                     /locus_tag="ACG8KR_001632"
     CDS             3909..4613
                     /locus_tag="ACG8KR_001632"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFQ0200999.1"
                     /translation="MTETTLYIVRHGKTMFNTIERVQGWCDTPLTKQGQEGIHYLGRG
                     LKNVDFSFAYSSDSGRAIETARIILGEHSKGKEIPYFIEKRIREWCFGSLEGGYDMEM
                     WGVIPRVLNFKTYDEMFTTEVSFEQIANAVYNLDTAGWAETYETLRDRVWTGFEDIAH
                     HCEKNGGGNVLVVSHGLTIAFLLNLIDPKQPVRAGLLNGSVTKVTYANGRFSIHGIND
                     TSYIDNGKTHSRDSFL"
     gene            4805..5638
                     /locus_tag="ACG8KR_001633"
     CDS             4805..5638
                     /locus_tag="ACG8KR_001633"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HFQ0201000.1"
                     /translation="MFFANIIKAVILGIIEGITEWVPISSTGHLILADEFIKLDASSQ
                     FMSMFNVVIQLGAILAVVVLYFHKLNPFSPSKSTVEKKDTWTLWFKVIVACIPAIIIG
                     LPLDDWLEANFHKFLPVAIVLIVYGIAFIIVEKRNKNKTPRWTSLNDFTYQAALLVGL
                     FQVLALVPGTSRSGATILGSILIGASRFVATEFSFFLGIPVMFGASGLKIVKYLAEGN
                     SFKMEETVVLLVGTFVSFVVSVLAIKFLLSYLKKNDFTAFGWYRIILGIILIGYWFIA
                     M"
     gene            5835..7763
                     /locus_tag="ACG8KR_001634"
     CDS             5835..7763
                     /locus_tag="ACG8KR_001634"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFQ0201001.1"
                     /translation="MTNQKKAFAATIGCLLFMIFGFIFDRFSLTITPLFYISAIIIGG
                     YKQTSEGLKELWNDRTLNVDLLMALAAIGACLIGNYFEGAMLTFIFCLSGALEEYTTN
                     KSQKEITALMNLQPQKAQLLKENGQMQEVDVSQLKVDNLVFVAKGAAVPIDGFLESTQ
                     ATIDESALSGESVPVEKYLGDEMYAGTLNQGNPITIRVTKTSSETVFAKIIQLVEEAQ
                     NTPTQTASFIERIENNYVKLILLAVPLMILLPHFFLGWSWDESFYRGMVLLVVASPCA
                     LVASATPASLAALSNSAKNGILIKGGIHLEKLSELKAIAFDKTGTLTKGKPVVTDSFF
                     FDEKDLAQQLLVAMEQKTTHPLAHAILQYFDCTIPEEIQYLPIEEITGFGLQTTYLGA
                     QWKVGKHAYDPETMIISKEIAEMIERLENQGKTVIYLSKDQQLIAVLGLLDIPKANTQ
                     QVISYFKSQNIHTSMITGDHSGTAKAIAEQVGIEHYYASCTPEEKTQLVKKENELYQV
                     NAMVGDGVNDAPALAAASLGVAMGQGTDAAMDIADIVLMKSDLNKFVYSHRLSLKMRR
                     IIKQNIIFSILVILLLIASNFLQFLNLPLGVVGHEGSTILVILNGLRLLRPLPDLSDS
                     SKKCKSCPLYKARLSSVK"
     gene            7901..8356
                     /locus_tag="ACG8KR_001635"
     CDS             7901..8356
                     /locus_tag="ACG8KR_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HFQ0201002.1"
                     /translation="MESWLFLGIILLVAILAKNQSLMIAAAFVLIMKLIPASRSFFPW
                     LQGKGINIGVTIITAAILIPIATGEIGLRDLLEAFKSPIGWVAVFCGGLVAVLSSKGV
                     GLIASDPQITVALVFGTILGVVFLRGIAAGPVIASGITYCILQVFALGK"
     gene            8436..8564
                     /locus_tag="ACG8KR_001636"
     CDS             8436..8564
                     /locus_tag="ACG8KR_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0201003.1"
                     /translation="MFEKLKYFIGTIGWFLLAVLLILGLAYLLFPDLIQNVFDTAF"
     gene            8826..9095
                     /locus_tag="ACG8KR_001637"
     CDS             8826..9095
                     /locus_tag="ACG8KR_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0201004.1"
                     /translation="METFEGLLIDTYVLQEQPMMLTGINLQLEDRVINGLLKDRYLAK
                     YILRLPLNKYVLEVSGKWNRRNQLVIKKMLIKNIDEYIKIIGTPE"
     gene            9495..9749
                     /gene="rpsT"
                     /locus_tag="ACG8KR_001638"
     CDS             9495..9749
                     /gene="rpsT"
                     /locus_tag="ACG8KR_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HFQ0201005.1"
                     /translation="MPNIESAIKRVRTSENANVKNSSQTSAMRTAIKKFEDAVASGAD
                     NVDALYKEAVKAIDMAESKGLIHKNKANRDKSRLSKKIAK"
     gene            complement(9838..10884)
                     /gene="holA"
                     /locus_tag="ACG8KR_001639"
     CDS             complement(9838..10884)
                     /gene="holA"
                     /locus_tag="ACG8KR_001639"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HFQ0201006.1"
                     /translation="MVSNLSIQAELQKIRKEKLASCYLILGTEKFLQDQVRTEIVKKI
                     QLEGEDDLNFLSFDMENASLDEVVAEAETLPFFGDQRLVFVENPYFLTGEKVNNGIEQ
                     NTDLLTAYLKEPLESTVLVFFAPYEKLDERKKVTKQLKKTAIIINAQQLNEKEVRQYL
                     MNTLENSSIKMERSAIDLFLRLTDLDLSKMMRELQKLVLYGQNQQQITVREVEELVPK
                     TLEHNIFDMTQYILKGKTEQALRLYEDLVMQGEETIKINAILLSQLRLFLQTKFLVKI
                     GYQQANIAETLKIHPYRVKLAMQEVKKFDEHLLVQLFDQLVEMDYQIKTGQIEKELSF
                     QLFVLRTGQLLRNR"
     gene            complement(10922..13195)
                     /locus_tag="ACG8KR_001640"
     CDS             complement(10922..13195)
                     /locus_tag="ACG8KR_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HFQ0201007.1"
                     /translation="MSSVLERHVRNRLIFPAITSMIICLLYYTDNWGYRLLLAGIFLW
                     FSLKEGRFIAYLCILCSITVSISYRLQEYSFLEEKTTSAQITVFTDTIRANGNFLTME
                     GRLSENKQKLQLSYQVNTEAELKTWLNFRGKGIELTAEGEFISPAKKRNLYNFDQEAY
                     FRNHRISGRFQIQKIEQITMQNHWRNWLQRKRGSFISFVQSEFPQKTSVYINSLLWGY
                     KDAGFREPEEIFRESGLLHLFSLSGLHIQFYLGWLYYLFRRIGWPLHVSIIPLSLLTI
                     CYVSIAGSSISVLRASLLFLLRLLVKLLNKHYSTMDLFAVVFWLLLLFDPRFLFHAGG
                     QLSFFMSFLFILISFDGDWLKKTASHVLFTMITMPLIVWHFYEWPILGSLFTLLIAPV
                     FKFLFLPGVLFLTLFNRWLPETLLLLIEEGLILFERIIDFSSGSSLIIGRLPLVLSGM
                     YIFGLLICMDRLKEQPVKHLFYVGLFSFIFLTLPFLRFSSTIAFIDVGQGDSIFLQSP
                     FRKETILIDTGGQLHFEREKWAQGMIRPPAERNIVPYLKGQGISVIDKLILTHDDTDH
                     VGELPTLSQHFTIKKIYIGWGAGRNEPLQRHLSEAQKNGTEIIEIKHGDRISGYYDLY
                     VLTPFEKGEGRNEDSIGLWMEYNNRRFLFLGDLNQAMEKQLLLIYPNLKADVVKLGHH
                     GSRTSSNTDFLSHIEAKHGIISCGVNNRYGHPHSEVLETLEENQIKTWRTDQQGMITY
                     RWHPVFHPHGLVETMID"
     gene            complement(13188..13727)
                     /locus_tag="ACG8KR_001641"
     CDS             complement(13188..13727)
                     /locus_tag="ACG8KR_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HFQ0201008.1"
                     /translation="MLEKSSKACDTNKEKVTDKSHNERIPWDQYFMAQAVLLSLRSTC
                     TRLEVGATLVREKRIIAGGYNGAVSGDVHCIDEGCYVVDGHCLRTIHAEMNALLQCAK
                     LGIPTEGSEIYVTHFPCLACTKALLQAGVKGINYLHDYRNDPYAQALIEQLGVSVHQV
                     ALDSQHFSKLQNELTGKHV"
     gene            complement(13863..14081)
                     /locus_tag="ACG8KR_001642"
     CDS             complement(13863..14081)
                     /locus_tag="ACG8KR_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFQ0201009.1"
                     /translation="MIVQLKKDYSSDHAFFITPPAVDETKQKYRNNKLIKEYISVLTE
                     VAKRHDCEVSIFIRNFLNKWIRRIFIMG"
     gene            complement(14078..14323)
                     /locus_tag="ACG8KR_001643"
     CDS             complement(14078..14323)
                     /locus_tag="ACG8KR_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HFQ0201010.1"
                     /translation="MIVRKETLKKPMLNVYLQNKISGIHIMNTAVSGNNSQALRERFA
                     KDVLSYTADKVFILIGTNDLAEHKQLSKETYQKICSG"
     gene            complement(14439..15167)
                     /locus_tag="ACG8KR_001644"
     CDS             complement(14439..15167)
                     /locus_tag="ACG8KR_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016925838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HFQ0201011.1"
                     /translation="MKGVMNDFYWSFFEKRTMIEKISKKILASPKLIIWMSMIVLIFC
                     IASGFLLSNLLFYRETEETAAKEILSSFSSSGEDPSAEGNDVEEDARPKVMYTDIKGS
                     VKEPGIYSFSSEERVYDVLKRAGGLLEEADSDRINFSAKIEDQQVLYIPAVGEELPEH
                     LDQLASSEGKQPTADSEPPKININTATPSELQQIPGIGSVKAQEIIRFREENGSFQKV
                     EDLQEISGIGEKTVEKLKNFVTIK"
BASE COUNT         4656 a         2953 c         2723 g         4927 t
ORIGIN      
        1 tagatgtatg gtaaatattt atatgtatat caggagggtt cctatggcaa aagatgctag
       61 cttcgacatt gtatcagaag tcaatacaga agaagtaaaa aatgcgattc agcaaacaac
      121 aaaagaattg tccaatcgct ttgacttcaa ggattctaca gtagaaatca aattagagaa
      181 cagcaatcta gttgtattga gcgatgatga tttcaaaatc gaacagatca aagatgtatt
      241 atttggtaag ctgatcaaaa gaaatgtgcc aatcaaaaat atccattttt ccgaatcaga
      301 acacgctcta ggtggcaaag ctcgacaaac tgccgatttg gtcaacggaa tcgatcgtga
      361 aaacgcgaag aaaatcacta cagctatcaa aaatgaaaaa ctgaaagtaa aagcacaaat
      421 ccaagaagat cagattagag tgacaggtaa gaaccgtgat gatctacaag ctgtgattgc
      481 tttattacgt aaacttgact taccaatcga gttgcaattc actaactatc gttaatcaaa
      541 aaacgaggct gacgtctgtc attcctcgtt tttttgattt actgcttctt ttgataacca
      601 aaaacgctgt attccttctt ccaattcccc aacacttcgt acttctcttg ctgtttgcca
      661 atgttgtggg aataaacgtc gataagaaaa actagaaaat cgttgatctg ccaatacaac
      721 gattccttga tccgccatgc cacgaatcac tcgaccacca gcttgcaaga ctttgttcat
      781 tccaggaagc tgataagcgt aagcaaatcc aaggtgttcc ttctcatcat aatatgattt
      841 gatcagttcc tgctcatgat tcatctgcgg caaacctaca cctacgatca tactgccgat
      901 caatcggtcc cctctcaaat cgattccttc tgaaaaaatc ccgccaagta cgcaaaatcc
      961 aatcaatgtt tcagcgggat ttgtttgaaa ttttgttaaa aatcgttcgc gttcttcctc
     1021 attcatgtcc gtttcttgga ttagcacatt gctgtcggga tattgctgtc tgaatgcttc
     1081 caccgcaaga tctaaatatt gataagaagg aaagaaaacg aaataattcc cgacctttcc
     1141 atcagcaaaa gcatgaatcg tctctactaa ttttgccaga ctgtgttccc tttttcgata
     1201 agtcgtctct aggtaatttg cgatgaaaac ttgctggttt ccttctggga aaggactcgg
     1261 cagtttgtaa gccagacttt tttgtccccc cagtgtttcc tgataataag agagtggtga
     1321 aaaactagca gaaaaaagaa tactgctttt tcctttatcc aacgactgct caagaaacaa
     1381 gcttggatca atacaaaact cttttactat cagatcgcga tacgtttttt cgactgttgt
     1441 ttcgtagtga tcatcataaa attcactgac cttcaaaaaa tgaagcaaat caaaataata
     1501 aggaagcaat tgttcttgtg atgggtgctc aggaaattct gccagccatt ctttaatttt
     1561 ttcgcttaat tgataacctg cctttactaa agattcagcc ggtgcttttt gatgatgata
     1621 atcccaatga tcttcttttg ctatttctcg gatcgaatca aattctttta atagtttgtt
     1681 gaaacggcga tgaagttttt tgaattcctt tggtatagct tccttacttc gtttcgtttt
     1741 ttcataagat agttctgccg aatacatctc tcgggaacga ttcaccaaat tatgtgcttc
     1801 gtcaatcaaa aacagatagt cctcgttttt ttcatcttca aaaaaacgtc ttaaataaac
     1861 agttggatca aataaataat tgtaatcccc aatgattacg tcacaccaga tactgacatc
     1921 taatgatagt tcgaaaggac aaagtgtatg ctttcttgca taagtttcga tgacttcacg
     1981 agtgatctga ttctcatgat tcaaaagatc ccataaacct tcattaatcc gattgtaata
     2041 accattggca tatgggcact gatctggatt acaggtcgtc tcatctaaaa agcagatctt
     2101 gtctttcgcc gtcaaagtga cactttttac ttcagctcct gtatctttta atgctgtcat
     2161 agcatcttca gctacttgtc tagttattgt tttagccgtt agataaaaga tcttttctcc
     2221 ctcttcttcc cctacagctt tcaaagcggg gaataacgta gagattgttt ttcctgttcc
     2281 ggtaggtgct tctacaaaca atttctgttt cgtacggatc gttttataag cagctaccgc
     2341 tagttcacgc tgacctttcc tatactcatc atgggggaat ttcaaagcca tcaaagaggc
     2401 attgcgtaca gtgcgccagt tttcttgaaa aacaagccat tctttataat cttcagtcag
     2461 ctttttaaaa aaagtttcta actgctctct tgtctgatgt tcaacctttc tcgtgatcac
     2521 ttcttctgcc gtctggaagt aagtcaattg cagatttatt tcttgtaaat catgttcttg
     2581 actatagata taggcataca cccttgcttg atgaaagaaa agttccactt gttctggttc
     2641 taaatcttca aatcgtggtt cggaggtttt gatttcatct atataataaa taccgtctct
     2701 tttgaaaata ccgtctgcac ggccttcaac gatcaggtaa tattcatcta attgaactgt
     2761 tgtttttaaa aagacttctt tttcatagtt ttctcccgca tctttttgga gcttgcggtg
     2821 gattctcgct ccttctaatg cagtatggtt acttttttta cgattgtcga tacttccacg
     2881 tcgcaaaata aattctacta agtgccgcac agatattttt gactgtttga tcatttccac
     2941 cacctctttc tctctaagat tacctgaaaa aacctagttt agcaaaaaga gctctctttc
     3001 caaacataaa aaaacaccct gctgattagg cagggatata ggatatagaa tttggatttt
     3061 tggatgaatc taatgtaaca aggacatatt aaaatgctat gaaaacttac tttccttctc
     3121 tttagaatat ccaaatactt ttcatatagt ctgataattc agataagtaa agagaaataa
     3181 gtacctgttt ttgtttccta gcaatgaatg cgttatacta atggatgaaa gaagttttgg
     3241 ctatcaagga ggaatgcaca tgcatatcga aagattaaaa acaggaatga ttgaagaaaa
     3301 ctgttatttg gtctacaacg aagaagcact tttgatcatt gaccctggag aagatgcgga
     3361 acgaatcaaa caagaaatca aaaaaacaaa ccaacagccg gtagcgattt tgcttaccca
     3421 tactcactac gatcatatcg gtgcagtgga acctttacgc gcgtattatc aaatcccagt
     3481 ttatgtcagc ccactagagc aaaaatggct aggtgatccg attttgaatc tttctggact
     3541 cggtagacat gatgatattg ctgatgttat tgtctcgcct gcagaatatg aatttgaaat
     3601 gaaaacttat actttaggaa acatgtcttt tcgcgtcgtc cctaccccag ggcattctat
     3661 aggaagcgtc agctttattt ttgatgattt tgtcgtttct ggcgacgccc tgtttagagg
     3721 aagtatcggt agaaccgatc tgtatacagg gaatttagaa caattacttc atagtattcg
     3781 gacccagctg tttgtattac ctgatgaatt tgcagtctat ccaggacatg gcgatgcaac
     3841 gacgattgaa caggaaaaaa ggacaaatcc ttttttcaat cgttaaaacg acttaggagg
     3901 agaataaaat gacagaaact acactttata tcgtacgaca cggaaaaacg atgttcaata
     3961 caattgaacg agtacaaggc tggtgtgata cacccctcac aaagcaagga caagaaggca
     4021 ttcattattt aggtcgcggg ttaaaaaatg tcgacttttc atttgcttat tctagtgaca
     4081 gtggtcgtgc aatcgaaact gcccgtatta ttctaggaga acattctaag ggaaaagaaa
     4141 ttccttattt tattgaaaaa aggattagag aatggtgttt cgggtctctt gaaggtggat
     4201 atgacatgga aatgtggggt gtgatccctc gtgtattgaa ttttaaaaca tatgatgaaa
     4261 tgtttaccac agaagtttct tttgaacaaa ttgctaatgc tgtatacaac ttagataccg
     4321 ctgggtgggc ggaaacttat gaaacattgc gagaccgtgt atggacagga tttgaagata
     4381 tcgcccatca ctgcgaaaaa aatggtggtg gaaatgtttt agtcgtatct catggtttga
     4441 cgatcgcctt tttgcttaat ctgatcgatc ctaaacagcc tgttcgtgct gggcttttaa
     4501 atggcagcgt aacaaaggtc acttatgcaa acggtcgatt ttctatccac ggcatcaatg
     4561 atacgtctta tatcgataac gggaaaacac atagtcgcga ctcgtttcta taatggggat
     4621 attttaattt ttaccagaga atcataaaag gcaaagtctg aacgagggct ttgtcttctt
     4681 ttttctcttc gtgtacttat ctgctttatt tctcaagttt ttcttaatat taaaaaacag
     4741 attgggtttc tcaaaaaata acggtatact ataagatgta tcttaattgg gaaggaagga
     4801 atttatgttt tttgcaaata ttatcaaagc agtgattctc ggtattattg aaggaataac
     4861 ggaatgggtg cccatcagta gtactggtca tttgatccta gcagatgaat tcatcaaact
     4921 ggatgccagt tcacaattta tgtcgatgtt caatgtcgtt atccaattag gggcgatctt
     4981 ggctgtcgtc gttctttatt ttcacaagtt aaacccattt tctccaagta aatcaacggt
     5041 agaaaaaaaa gatacgtgga cactttggtt taaagtgatc gttgcttgca taccagccat
     5101 catcattggt ttgcctttgg atgattggtt agaagcaaat ttccacaagt tcttaccagt
     5161 tgccattgtt ttgatcgttt acggtattgc ctttattatt gtcgaaaaac gcaacaaaaa
     5221 caaaacacct cgttggactt ctctgaatga ctttacgtac caagcagctc ttcttgttgg
     5281 gttattccaa gtcttggcac ttgttcccgg aacatcccgt tctggcgcaa cgatcctagg
     5341 atctatcttg attggtgctt cgcgttttgt cgctacagag ttttcatttt tcttagggat
     5401 tcctgtcatg tttggcgcaa gcggcttgaa gatcgtcaaa tatttagctg aagggaattc
     5461 attcaaaatg gaagaaactg ttgttcttct tgtaggaaca ttcgtatctt ttgtggtttc
     5521 cgtcttagct atcaaattct tactaagcta tttgaagaaa aacgacttta ccgcttttgg
     5581 ctggtatcgt attatccttg gtatcatttt gattggttat tggtttattg caatgtagaa
     5641 aaaaccagaa cacttctctt atttgagaaa tgctctggtt ttttattttc tttaataata
     5701 atcattataa ataataaaaa caacatcaag cttaatcacc aaatgataac cattatcgtt
     5761 ctcatttagc tgttattatt atcaattaca tttttattat gctatccttg ttttgacaag
     5821 gaggtctaat aacgatgact aatcagaaaa aagcttttgc agcaacaatc ggctgccttt
     5881 tatttatgat cttcggtttt atcttcgacc gattctctct taccatcacc ccactttttt
     5941 atatcagtgc cattatcatc ggtggatata aacaaacatc ggaaggcttg aaagaattgt
     6001 ggaatgatcg gacactgaat gtggatttat tgatggcttt agctgctatt ggtgcctgtt
     6061 tgatcggtaa ttactttgaa ggagcaatgt tgacattcat cttttgttta agcggtgcat
     6121 tagaggaata cacaactaat aaaagtcaaa aagaaattac tgctttgatg aatcttcaac
     6181 cacaaaaagc ccaattatta aaagaaaatg gacaaatgca agaagtagat gtttctcaac
     6241 taaaggtgga taacttggta tttgtcgcta aaggagcagc tgttccgata gatggctttt
     6301 tagaaagcac acaggcaacg atcgatgagt ctgcattaag cggtgaatct gtgcctgtag
     6361 aaaaatattt aggcgatgag atgtatgcag gaacgctgaa tcaaggaaat cccatcacga
     6421 ttcgagtaac aaaaacgagt tccgaaactg tctttgctaa gattatccaa ttagtagaag
     6481 aagcgcagaa tacgccaact caaacagctt catttatcga acgaatcgaa aataattatg
     6541 taaaattgat tttgcttgct gttcctctaa tgatccttct cccccacttt ttcctaggtt
     6601 ggagttggga tgaaagcttt tatcgaggaa tggtattatt agttgtggct tctccttgtg
     6661 cgcttgtcgc atctgctaca ccagcttctc tagctgctct ttcaaattct gcaaaaaatg
     6721 ggattttaat caaaggcggt atccatttag aaaagctttc tgaattaaaa gcgattgcct
     6781 ttgataaaac aggaacgctc actaaaggaa aaccagttgt aactgacagc tttttctttg
     6841 atgaaaagga tttggcacaa caacttcttg ttgctatgga acaaaaaacg acgcatccac
     6901 ttgctcatgc gattttgcaa tatttcgatt gcacaatacc tgaagaaatc caatacttac
     6961 caatagaaga aattactggt tttggattac aaactaccta tttaggtgcg caatggaaag
     7021 ttggcaagca cgcatatgat cctgagacga tgataatcag caaagagatt gcggaaatga
     7081 tcgaacgttt ggaaaatcaa ggcaaaaccg tgatttatct ttctaaagat cagcagttaa
     7141 ttgctgtttt aggtctgcta gatattccaa aagcaaatac ccaacaagta atttcctact
     7201 ttaaatccca aaacatccat acaagtatga taaccggcga ccattctggc acagcaaagg
     7261 ccattgctga acaagtaggg attgaacatt actatgcaag ctgtactccg gaagaaaaaa
     7321 cacaactagt caaaaaagag aatgaacttt accaagtaaa tgcgatggtt ggtgatggag
     7381 tcaatgatgc gcctgcttta gcagccgctt ctttaggtgt cgctatgggg caagggacag
     7441 atgccgctat ggatatcgca gacatcgtct taatgaaaag cgacttaaat aaatttgtct
     7501 acagtcatcg gctatcttta aaaatgcggc ggatcatcaa acaaaatatt attttttcga
     7561 ttcttgttat tctcttgttg attgcttcta acttcctgca atttttaaat ctacctcttg
     7621 gagttgtagg acacgaagga agcacaatat tggttattct gaatggcttg cgtttactaa
     7681 gacctttacc tgatctttct gattcttcaa aaaaatgtaa gtcttgtcca ctatacaaag
     7741 cgcggctgtc ttctgtcaaa taagcaaagt gtttttttat gattttgttt attttatgaa
     7801 aaaaacagct tatgaatagg tcttgaagga tgaattccta cttttgtaac tttctaaaaa
     7861 catgttatga ttgtttcgaa gaaagtgagg aatatatttc atggaaagtt ggcttttttt
     7921 aggaattatt ttacttgtag caattttagc taaaaatcaa agtctgatga tagcggcagc
     7981 ttttgttttg attatgaagc tcataccagc ctcccgttcc tttttccctt ggcttcaagg
     8041 caaaggaatc aatattgggg tgacgatcat cacagcggct attctaatcc ctatcgcaac
     8101 aggagaaatc ggtctacgtg atctattaga agcctttaaa tcccctattg gctgggtagc
     8161 tgttttttgc ggtgggctag tggctgtctt atcttccaag ggtgtaggat tgattgcttc
     8221 tgatccacaa atcactgttg cacttgtttt cggcacgatt ttaggtgttg tcttcttaag
     8281 aggaatcgcc gccggaccgg tcattgcttc tggtatcact tactgtatct tacaagtatt
     8341 tgctttagga aaatagaaca aattttgtct attttaggag attcttaaca cccccaattt
     8401 actgatccgt gatatactga caacgaggtg atacaatgtt cgaaaaatta aagtatttca
     8461 taggaacaat cggctggttt cttctagcgg tacttttgat tctgggctta gcttatctat
     8521 tatttcctga tttgatacaa aatgtgtttg atactgcttt ttgattgacc tattaaaacg
     8581 atatagggga tgctcctgcg atgcaggggc tttttttaat caatgttttg taataattta
     8641 ggccgtgcca atgagacttc ctatagaatt cgatgctctt ttttcttttt ggctcttctg
     8701 ctcttttttg ttatactaga ttacagtgga caaaagaaag aatgatcgca tccacttcaa
     8761 cgaatatcaa aatggaagaa ttagtgcctg ttttcaggtt actttgaaaa gaaaggaaga
     8821 cgcttgtgga aacttttgaa ggtcttttaa ttgatacgta cgttttacaa gaacagccaa
     8881 tgatgctgac tgggatcaac ttgcagcttg aggaccgtgt gatcaatggc ctgcttaaag
     8941 atcgctattt ggcaaaatat atcttacgtt tgcccctaaa taaatatgtg ctggaagtaa
     9001 gtggcaaatg gaaccgtcgg aaccagttgg tcataaaaaa aatgttgata aaaaatatcg
     9061 atgaatacat taaaataatc ggtacaccag aatagccttt actgatcaaa ggatactggt
     9121 tataaaataa ctgcagtttc ttaatagaaa aacaaagaac aacagggaat cgtatccctt
     9181 ttcaaaaaaa actttgcaaa cggttctctt tgttatataa taacttagta ccaaacattc
     9241 ccattcaaac tttgtttaaa tagataaata aaaatctagt ttggtttgac gctattgcct
     9301 cccacaatag cgtctttttg atttttttca gcttgtacta tccgttttta ctgaaaaaaa
     9361 atcctaataa aagactgtta aggaaaaaaa cttgtcatgt tcatattgca aagcacgctg
     9421 cctttaggta taataatgaa tgttgaatta tgcggtaaca cgcgtaagaa cttaagttat
     9481 cggaggtgaa actaatgcca aacattgaat ctgcaatcaa acgtgttcgt acaagcgaaa
     9541 acgctaatgt gaaaaattca tctcaaacaa gcgctatgcg tactgcaatc aaaaaatttg
     9601 aagatgctgt agcttcaggt gctgacaacg tagatgcgtt atataaagaa gctgttaaag
     9661 ctattgatat ggctgaatca aaaggtctaa tccacaaaaa caaagctaac cgcgacaaat
     9721 ctcgtttaag caaaaaaatc gctaaataaa agaatcggct tcggccgatt tttttatttg
     9781 tcaaaaaaag gacaaaaaca aacgaccttc ccgcatgctt tgccctcgtc tttcttctta
     9841 tcgatttctt aacaactgcc ctgtgcgcaa aacgaatagt tgaaaactta attctttttc
     9901 gatttgtccc gttttgattt gataatccat ctcaactaat tgatcaaaca actgtaccag
     9961 caaatgttca tcgaattttt ttacttcctg catggcaagt tttacacgat atggatggat
    10021 tttcaatgtt tcagcaatat ttgcctgctg ataaccaatc tttacaagaa attttgtctg
    10081 taaaaataaa cgtaattgag aaagcaaaat cgcattaatt ttgattgttt cttccccttg
    10141 cataactaaa tcttcgtata aacgcaaagc ttgttccgtc ttccctttta aaatatattg
    10201 ggtcatgtca aaaatattat gttctaacgt tttaggaact aattcttcta cttctcttac
    10261 cgtgatttgc tgttgatttt gtccataaag gaccaatttc tgtaactctc tcatcatttt
    10321 ggataaatct aagtcggtca acctcaagaa taaatcaatt gcacttcgtt ccattttgat
    10381 tgaggaattc tctaaggtat tcatcagata ttgacgtact tctttttcat tcaactgttg
    10441 ggcattgatg ataattgctg tttttttcaa ttgtttcgtt acttttttcc gttcatccaa
    10501 cttttcatat ggcgcaaaga atactaatac agtcgattct aatggttctt tcaaataagc
    10561 cgtcaataaa tcagtatttt gttcgattcc attgtttact ttttcacctg ttaaaaaata
    10621 aggattctct acgaatacta accgttgatc tccgaaaaac ggcaacgtct ctgcttcagc
    10681 tactacctca tctaaacttg cattttccat gtcaaaactt aaaaaattta agtcatcttc
    10741 gccctctaat tggatttttt tgacaatttc tgtccgaact tgatcttgga gaaacttttc
    10801 tgttcctaaa atcaagtaac aagatgcaag tttttcttta cgtatttttt gtaactctgc
    10861 ttgtatactc aaattactca ccactttcaa aaattatcct accatgagca gatgagaatt
    10921 actagtctat cattgtttcc actagcccat gaggatgaaa aactggatgc cagcgatagg
    10981 tgatcattcc ttgttgatcc gttcgccatg tttttatctg attttcttct agtgtctcca
    11041 atacttcgct gtgcgggtga ccataacgat tatttacgcc gcaggatata atcccatgtt
    11101 ttgcttcaat atgggaaaga aaatctgtgt tacttgacgt acggctaccg tgatgaccta
    11161 atttgactac atcggctttc agattaggat aaatcaaaag caattgtttt tccattgctt
    11221 gattcaaatc tcctaaaaat aaaaagcgcc tattgttata ttccatccat agaccaatcg
    11281 aatcttcatt tcttccctct cccttttcaa aaggagtcaa tacataaaga tcataataac
    11341 cgctgatcct gtccccatgt ttgatttcaa tgatctcagt accatttttt tgagcttcag
    11401 ataaatgcct ttgtaatggc tcatttcgtc cagcgcccca accgatataa atttttttga
    11461 tagtaaaatg ctgagataac gtgggtaatt ctcccacatg atccgtatcg tcgtgtgtca
    11521 agatgagttt atcgattaca cttatcccct gaccttttag atatggaacg atatttcttt
    11581 cagcaggagg acgtatcata ccttgcgccc acttttctct ttcaaagtgt aactgacctc
    11641 cagtatcgat caaaatcgtt tcttttcgaa aaggactttg caaaaagata ctgtctcctt
    11701 gaccgacatc gataaaagcg atcgttgaag aaaagcgtaa aaatggcaaa gtcaaaaaaa
    11761 taaaagaaaa aagaccaaca taaaacaagt gtttcacagg ttgttctttc aacctatcca
    11821 tacaaatcag taatccgaaa atatacatcc cagatagcac aaggggcaaa cgtcctataa
    11881 tgagagacga ccctgaagaa aaatcgatta ttcgctcaaa taagattaat ccctcttcta
    11941 tcagcaaaag taaagtttcc ggcagccagc gattgaagag tgtgagaaat aacacaccag
    12001 gaagaaaaag aaacttgaat accggtgcaa tcagtaatgt aaaaagacta cctaaaatag
    12061 gccactcata aaaatgccat acaatcaacg gcatcgtgat catagtaaac aggacgtgag
    12121 aggccgtttt tttcaaccaa tctccatcaa aagaaatcag gataaacaaa aaactcatga
    12181 aaaaactaag ctgtccaccc gcatgaaaaa gaaatcgagg atcaaatagt aaaagaagcc
    12241 aaaagacaac tgcaaataag tccatggtgg aataatgctt attcagcaat ttgactagca
    12301 atcgcaataa aaacagcaaa ctagcacgca aaacagaaat agaacttcct gcaatagaaa
    12361 cataacagat agttagtaga ctcaacggaa taatactgac gtgtagtggc catccgatac
    12421 gtcgaaataa ataatacagc caccctaaat aaaattggat gtgtagaccc gatagactga
    12481 acagatgcag caaaccactc tccctaaaaa tttcttctgg ttctctgaat cctgcatctt
    12541 tatagcccca taataaagaa ttaatatata cacttgtttt ttgcggaaat tcagattgga
    12601 caaaggagat aaaagatcct ctttttctct gaagccagtt tcgccagtga ttttgcatgg
    12661 tgatctgttc aatcttttga atttggaacc gtccagaaat tctatggttc ctgaagtaag
    12721 cttcttggtc aaaattataa agattccgct tttttgctgg agagatgaac tcgccttcag
    12781 cagtcaattc tattcccttt cctcggaagt taagccatgt tttcaattct gcttctgtgt
    12841 ttacttgata agaaagctgg agcttttgtt tattctctga taaccttcct tccattgtca
    12901 aaaaattacc atttgcacga atcgtatctg taaatacagt aatttgcgca gatgttgttt
    12961 tttcctctaa aaatgaatac tcttgtagcc tataagaaat ggataccgtt atgctacaca
    13021 aaatacacaa gtacgcgata aaacgtcctt cttttagcga aaaccataaa aaaattccgg
    13081 caagaagcaa acgataaccc caattgtcag tgtaatacaa caaacagata atcatcgacg
    13141 taattgccgg aaaaatcaac cgatttcgaa catgcctttc taaaacgcta gacatgtttt
    13201 cctgttaatt cattttgaag ttttgaaaaa tgctgtgagt ctaaagcaac ttggtgtaca
    13261 gatacaccta attgttcgat caacgcttgg gcataaggat catttcgata gtcatggaga
    13321 taattgatcc ctttcacacc agcttgcaac aaagcctttg tacaggccaa acatggaaaa
    13381 tgtgtgacat agatttctga accttccgtc gggataccaa gttttgcaca ctgtaggagt
    13441 gcattcatct ctgcatggat cgttcgcaag cagtgtccat ccacgacgta acacccttca
    13501 tctatgcagt ggacatctcc actgactgct ccattatagc cgccagcaat gatccttttt
    13561 tctcgaacga gtgtcgctcc tacttctaaa cgtgtacacg tactacgtaa agataacagt
    13621 actgcctgag ccataaagta ctggtcccaa ggaatgcgtt cattatgtga cttatcagta
    13681 actttttctt tattcgtatc gcatgctttt gaagattttt ctaacatgtt ttatattcct
    13741 ccgttttttc aagttgatta aaaagtactc attgcttaaa caaaatattc tttaaatata
    13801 acgaatcatc ctgtctgaca aaagatccta cccttcactg ccgaaatgaa gtccgtcatc
    13861 cattagccca ttataaaaat tcttcgaatc catttgttca agaaattccg tataaaaatc
    13921 gatacttcac aatcatggcg ttttgctact tctgtgagaa cagagatata ttctttgatc
    13981 aacttgttat tccgatattt ttgttttgtc tcatcgactg ctggtggcgt gataaagaaa
    14041 gcatgatcag aactatagtc ttttttcagc tgaactatca tccactgcag attttttggt
    14101 acgtttcttt agaaagttgt ttatgctctg ctagatcatt cgtcccaatg agaataaata
    14161 ctttatctgc tgtatagcta agtacatctt tggcaaagcg ttctcgtaga gcttgactgt
    14221 tattgccgct gacagccgta ttcataatat gaatcccaga tattttattt tggagataga
    14281 cattcagcat tggttttttt aatgtttctt tgcgaacaat caagctattt cctatccaaa
    14341 aatactacct cctcgtcctg ccaagctctt agaattggat tattcttagt atccattagc
    14401 gtccatttat tgctcgtgcc agtcgatttt gaatcattct atttaattgt aacgaagttt
    14461 tttagttttt caactgtctt ttcaccgata ccagaaatct cctgcaggtc ttccactttc
    14521 tggaaggatc cgttttcttc tctaaatcga atgatttctt gcgctttgac tgatccgatc
    14581 ccaggaatct gctgaagttc tgatggagtg gctgtattga tatttatttt gggaggctcg
    14641 gaatctgctg taggctgttt tccttccgag gatgccaact gatcaagatg ttctggcagc
    14701 tcttcaccta cagcagggat atataggact tgctgatctt ctatcttcgc agaaaagttg
    14761 attcgatcgc tatccgcttc ttctaataat ccacctgcac gctttagtac atcatagacc
    14821 cgttcttctg aagaaaagga atagatacca ggctccttca cacttccttt gatatcggtg
    14881 tacatgacct ttggacgagc atcctcttct acatcgtttc cttcagctga tggatcttct
    14941 ccactggaac taaacgaaga aagtatttct tttgcagcag tctcttctgt ttctctataa
    15001 aatagcaaat ttgataataa aaaaccactt gcgatacaaa agataagaac tatcatagac
    15061 atccaaatga tcaatttggg tgaagctagt attttcttgc tgattttttc tatcattgtt
    15121 cttttttcaa agaatgacca gtaaaaatca ttcattactc ctttcattta aaattgaaga
    15181 aacagtgagc tctggcaaaa tcgtcccttc aacatctaat tacgtcaatt ttttcttttg
    15241 tttttatttt tatcgtgaa
//
LOCUS       DAZKLD010000066        15132 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.066, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000066 DAZKLD010000000
VERSION     DAZKLD010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15132)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15132)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15132
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.066"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(312..878)
                     /locus_tag="ACG8KR_001645"
     CDS             complement(312..878)
                     /locus_tag="ACG8KR_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFQ0201012.1"
                     /translation="MLQTALHFSHTLLQEILQPGDHVVDATMGNGHDTVFLAEHIGKT
                     GHVYSFDIQQQAIDATRERLEQRQLEERVSLFLQGHETLGEVIAEQQNLKAGIFNLGY
                     LPKSDKAIITMPETTRTAMEEILKRLVPRGRLILVVYYGHEGGEKELDMVDSFCSKLP
                     QETYNVLNYRFINQKNQPPILYCIEKKR"
     gene            complement(878..1834)
                     /locus_tag="ACG8KR_001646"
     CDS             complement(878..1834)
                     /locus_tag="ACG8KR_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390904.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HFQ0201013.1"
                     /translation="MTFPYSEGNKRYHSWNYALRNEFGGKIFKVPIDGGFDCPNRDGT
                     VAHGGCTFCSVSGSGDMIVAPEDPLPIQFQKEIDMMHQKWPHVQQYIVYFQNFTNTHA
                     PVEIIKERFEQVVNLPGVVGLSVGTRPDCLPDEVVDYLAELNERMYLWVELGLQTTFE
                     ETSKLINRAHDYQTYLDGVAKLRKHNIRVCTHLINGLPGESLEMMKENVRRTILDSDI
                     QGIKLHLMHLMRKTRMLRDYHEGRLQLMSRPDYVNVICDQLEMIPKEIIIHRLTGDAP
                     WDSLIGPMWSLKKWEVLNAIDEELLRRDSFQGKYDVRKKVSV"
     gene            complement(1834..2490)
                     /locus_tag="ACG8KR_001647"
     CDS             complement(1834..2490)
                     /locus_tag="ACG8KR_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HFQ0201014.1"
                     /translation="MKYKTSFQVAGSFFLVLFFILGWIVKFHLSWLSGFDQWFTGIVR
                     ASYPDLNSYQLFMTKFGNPLTVIILFACAALWMWYKKKRAETFWFVSGFIVIAGIINP
                     VIKLFFMRERPSLAHLVVETSYSFPSGHAAGSMILYGSLLFLLPAFVQKRTLRTLLQL
                     LMMFIIVSVGISRIYLGVHFPTDIIGGYLLSLSWLCLTYPVYREKRKQIDNSYRKKDF
                     "
     gene            2860..4878
                     /locus_tag="ACG8KR_001648"
     CDS             2860..4878
                     /locus_tag="ACG8KR_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFQ0201015.1"
                     /translation="MENIARKERRRLEQTKRFRKAKRGAAIVGTAMVGCSVAAPLVQP
                     VPVQADETPVQFGARINTAAFITEIATYAQPIAQANDLYASVMIAQAVVESGWGSSAL
                     SQAPYYNLFGIKGSYQGQTVYMDTLEFLNNQWVNKKEPFRQYPSFAESFNDNAYVLRN
                     TSFGNGYYYAGTWKSNTKSYTDATACLTGRYATDPGYAGKLNNIIATYGLTQYDTPAS
                     GNAGGGATTGGNNTGSAGSTGGSSNSGNTNSGSATSTTYTVQSGDSVWGISNKFGITM
                     DQLIQWNNIQNNFIYPGQKLTIKGSQENGSSTNNSGNNTNSSGNAGTSNGGQTTGAKY
                     TVQSGDSVWKISNDHGITMAQLIEWNNIKNNFVYPGQQLIVSNGGSSNAGANTGSNTG
                     NTSGSSSSSSSSSNTASGTKYTVKAGDSVWSVSNKYGITMAQLIQWNNIQNNFIYPGQ
                     QLTVSNSGSTNSTSGNTGSSTNTNNSSSVNTGSSSSQGTYTVKAGESVWSVANKNGIT
                     MDQLIEWNNIKNNFIYPGQQLIVKGGTSANTNTGSTTSAAKPNTPNTSATTSTSSTGN
                     TMYTVKAGESVWSVANKHHITMDQLIEWNNIKNNFIYPGQQVIVKKGSSQSTNQQSVT
                     GSKTYTVKSGESVWSVADSHGITMAQLIEWNNIKNNFIYPGQTLIVAK"
     misc_binding    4948..5154
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            5305..7719
                     /gene="leuS"
                     /locus_tag="ACG8KR_001649"
     CDS             5305..7719
                     /gene="leuS"
                     /locus_tag="ACG8KR_001649"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HFQ0201016.1"
                     /translation="MSYNHKEIEKKWQKYWAKTNTFNTHDDPEKPKFYALDMFPYPSG
                     QGLHVGHPEGYTATDILSRVKRSQGYNVLHPMGWDAFGLPAEQYALDTGNDPAEFTQK
                     NIETFRRQINSLGFSYDWNREVNTTDPEYYKWTQWIFTKLYEKGLAYEAEVAVNWVPE
                     LGTVISNEEVIDGKSERGGYDVVRKPMRQWMLKITAYADRLLDDLELVDWPESIKEMQ
                     RNWIGRSVGANIEFKVAGTDKSYTVFTTRPDTLFGATYSVLAPELDLVREITTPEQKA
                     AVEAYIEETAKKSDLKRTDLAKEKTGVFTGAYAINPVNGKEIPIWIADYVLSSYGTGA
                     IMAVPAHDERDFEFAQTFGLEILPVIEGGDVEKAAYTEDGTHINSEFLDGMNKQEAID
                     KMNEWLEENGVGKKEVSYRLRDWLFSRQRYWGEPIPIIHWEDGTVTAVPEEELPLRLP
                     KTKNIKPSGTGESPLANIEEWVNVVDPVTGKKGRRETNTMPQWAGSSWYYLRYIDPHN
                     KKELADYEKLKRWLPVDIYIGGAEHAVLHLLYARFWHKFLYDIGVVPTKEPFQKLYNQ
                     GMILGENNEKMSKSRGNVVNPDDVVETYGADTLRMYEMFMGPLDASIAWSENGLEGSR
                     KFLDRVWRLIVDENNKMRDRITTLNDGKLDKVYHQTVKKVTEDYENLHFNTAISQLMV
                     FINEAYKVDALPYEYIEGFVQLLAPIAPHMGEELWAILGNDGGISYAPWPTYDEAALV
                     EDEVEVVFQVNGKVRAKSNVPRDLGKDELEKAALANEIVQEYIEGKTVRKVIAVPNKL
                     VNIVAN"
     gene            8067..9620
                     /locus_tag="ACG8KR_001650"
     CDS             8067..9620
                     /locus_tag="ACG8KR_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WD40/YVTN/BNR-like repeat-containing protein"
                     /protein_id="HFQ0201017.1"
                     /translation="MDQELFIIEVLSAKQKIIEKAKKRAKRIMFKLLSLVFFSGLLFF
                     GAVSFLYRHMSAVVRSAEGMQKLNNLYHFSWGVGGITIVVLIVFLLLIWLRYHQLSRH
                     FDQWLSQRAQQFLHNKPTSQDDDYLYFEKTSTSQAFRIQKSDCQLLLRSLDDLPIYLG
                     ESKSKFGMNTFHIFLLENEPRWEKSQSSPGGYLTTKMSTLVTGFFLILLAGTIYYGRY
                     TQPKMSSHINAASHMTMNSTNDTHIQKTNEGLLADPKQKVNLTTQKINDLKLDTETHS
                     LYMTTDSGANWVFVPIDSQWLRFGEYTLTTGLIPEGYWMDNTYDISADFSWFIYSSGD
                     DVRLLTSRDNGKTWQTSIITQSVQPIRYRKAVFYGNDGLFICTSASGMSAETLYIYTT
                     MDGGITWIKSGSTMINQPVQNVSFVTTSLGFVSTRESLFYTNNGGRSFKESVLSIPEN
                     YQTGGIDLFQSPNEVTQISANTLETKFYLLKKGPIDAGKMFACLFRSTDNGETWQFVQ
                     QLSQVEQGD"
     gene            complement(10001..11785)
                     /locus_tag="ACG8KR_001651"
     CDS             complement(10001..11785)
                     /locus_tag="ACG8KR_001651"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0201018.1"
                     /translation="MEKKKTLNTRALIRFWKMIKPEHKYFYGLFLCSLFGNLLVVAMP
                     LIMGIGIDDLLSEIRKTGIGQLSFSDIKQALLFPVLLLIGCSVLSSITSFIQERTMAA
                     LSEKITLRVRKEITKKFKTLPMSFFDNHQVGDIISRTTTGLNQLSQVLLTGINQFFTS
                     VVTIAAAGLMLFYIESKLTLLVLLLIVGSSIVTTKIANKNKQISEQNQYELGVLNNRM
                     EEILAGNLVVKTFNQQAHAEAAIHEVNKKQYNAFKRAQFMNCAIYPAIRFINQLAFII
                     SAVFGAMLVLSGGVTIGFLQAYLQYINQISEPISTASYVINSIQSAMAAIDRIFEILD
                     ETDEIPDLPDARLLEQPKGKIAFKNVQFGYTSDKILMNQVDFSVKPKQTVAIVGPTGA
                     GKTTLVNLLMRFYELNKGKIEFDGLDITKLSRGELRRSFGMVLQNTWLFEGTVADNIA
                     YGKMDATREQIIEAAEIAQCDHFIRTLPDGYDTIISSENGTLSQGQQQLLTIARVILA
                     NPPVVILDEATSSVDTRTEALIQQAMEAVTKDRTSFVIAHRLSTIENADMILVMKNGD
                     IVEKGTHSELLQSPTLYASLYNSQFQIA"
     gene            complement(11800..13521)
                     /locus_tag="ACG8KR_001652"
     CDS             complement(11800..13521)
                     /locus_tag="ACG8KR_001652"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0201019.1"
                     /translation="MSILKTFLQKNKGLALLTFVMICLEIAGTLGVPKLVAKLIDTGI
                     ASGEASVIKKIGVEMLLVAFLGTIAAVASSYFSALASARFGFQTREKYFRKFQQLSMK
                     DISRFSSGSLLTRMTNDVDNVQQMIVLFCQMILPAPVICVFTILMMFRYSLLLTWVTL
                     ISVVFYVWIVYRLMKRGAPLSLSIQPKMDRITITLREFFTGINMIRAFDNQSYEEKRT
                     NKSFADYANQMIKVNRIFAWVTPIAFLLMGIVYASILWFGGNLVAEGALQIGVVTAVV
                     EYSMLTLAYLMIAAMVLVIIPKSFASLRRIEEILQAEIEIEEETIKTVAEPTFDQTNA
                     AAFEHVTFKYTEISDPVLEDIDFTIPKGKTTAIVGGTGSGKSSLAKLLLRLSDPTIGK
                     VTLGGIPLTQMTQEQIRSHISYVPQKAFLFSGTILSNLQMGNEYASTEELSKAIKIAQ
                     LEELVDTLPDGLDSFVAQGGSNYSGGQKQRICIARALIKPAEIYVFDDSFSALDYKTD
                     AALRAALHKHMSEKTLVIVAQRLSTIQQADNIIVLDEGKIVGQGTHEELLSNCQTYQE
                     FAKSQGI"
     gene            13621..14382
                     /locus_tag="ACG8KR_001653"
     CDS             13621..14382
                     /locus_tag="ACG8KR_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFQ0201020.1"
                     /translation="MKKMYTIGQVSELFDLPKSTIRYWDEQGLIRSSRQEENDYRLFD
                     IDDIFMLYDIVFYRKLDIPIKQMKNLYGKTLTELYETLDETERRIHKELVVMKQKQKE
                     IREQKKQLKLMIDTNEEEFPVEEIPFDCMISTEFEDIVEIKKFLPNYSSFGMMSMPAS
                     SSQTMYGFFIDPSEVHLFTQDVIWEKKDTAVYRRFLLKSEMNHAERNNILEIRERMYE
                     KGWKTGEVIGQYLLTNTDENNIRTEYYHAWIEMKK"
BASE COUNT         4929 a         2752 c         2981 g         4470 t
ORIGIN      
        1 aatataaaat cttttaaaaa tgcttatata tcaatgtttt tgccattctt ttatatatct
       61 gttatatgtc aaaaaaatgt gttcaatttc aatttgtatt tgaataaaga ggttgtgaaa
      121 aagctgtcct gcatcaagta ataagaacag ccaatcaaaa acagcttctc atgtttttga
      181 tttggttgga cttattaccg cagatataag cttttgagca ccgtgaataa agaggttatg
      241 aaatcaacgc tctaacctga gtattagaga aaaaaacgca aaacatatca tttatgtttt
      301 gcgtttttta tttatctttt tttctcaata caatataaaa taggcggctg gttcttttgg
      361 ttgataaacc gataattcag cacattatat gtttcctgtg gcagtttgga acaaaaagaa
      421 tcaaccatat ccagttcttt ttctccacct tcatgaccat agtagacgac aaggatcaat
      481 cgtccgcgag gcacaagtcg tttcaatatt tcttccatag ctgtgcgtgt agtctctggc
      541 atagtgatga ttgctttatc actttttggg agatacccta gattgaaaat ccctgctttg
      601 agattttgtt gttcagctat cacttctcct aacgtttcgt ggccttgtag aaacaatgag
      661 acacgttctt ctaactgacg ctgttctagc ctttcacgag tagcatcgat tgcttgttgc
      721 tggatatcaa aactatagac atgaccagtt tttccgatgt gttctgctag aaaaaccgta
      781 tcgtggccat tgcccatcgt tgcatctacg acgtgatccc ctggttgcaa gatttcttgc
      841 agtaatgtat ggctgaaatg caaagcagtt tgcaacatca gacgctcacc tttttccgta
      901 catcatattt tccttggaag ctatctctgc gaagaagttc ttcatcaatt gcattcaaga
      961 cttcccattt tttcaagctc cacattggtc caatcaagga atcccacggc gcatcccctg
     1021 tcaaacgatg gatgatgatc tcttttggta tcatttccag ctggtcgcaa atgacattga
     1081 catagtcagg acggctcatt aattgaaggc gaccttcatg atagtcccgc aacattctcg
     1141 ttttgcgcat cagatgcatc aaatggagct tgattccttg gatatctgaa tctaaaatgg
     1201 tgcgtctgac attttctttc atcatttcta aactttcgcc tggtaatccg ttgatcaaat
     1261 gcgtacaaac acggatatta tgtttgcgta atttagccac gccatctaaa taagtttgat
     1321 aatcatgtgc tcggttgatc agttttgaag tttcttcaaa agttgtttgc aatccaagtt
     1381 ctacccataa atacatccgc tcgttcagtt cagcaaggta atcaactact tcatctggca
     1441 gacaatccgg tcgtgtaccg actgataatc ctacgacacc tggtaaattg actacttgct
     1501 caaatcgttc tttgattatt tcaactggcg catgggtgtt tgtaaaattt tggaaataaa
     1561 cgatgtattg ttggacatga ggccattttt gatgcatcat gtcgatttct ttctggaatt
     1621 ggattggaag agggtcttca ggtgcaacga tcatgtctcc cgaaccagat acactgcaaa
     1681 aagtacaacc gccatgggca acagtcccat ctcgattcgg acagtcaaat cctccatcga
     1741 tcggcacctt gaaaattttc ccgccaaact catttcgtaa agcgtaattc caagaatgat
     1801 atcttttatt tccttctgaa taaggaaaag tcattaaaag tccttctttc tgtaagagtt
     1861 atctatttgt tttctttttt ctctatacac tggataagtc aggcacagcc aagaaagact
     1921 gagtaagtag ccgccgatga tatcagtagg aaaatgaacg cccagataaa tgcggcttat
     1981 cccgacactt actataataa acatcatcag caattgaagc aatgtgcgta aggttctttt
     2041 ttgaacaaaa gctggaagca gaaatagtaa agaaccgtaa agaatcatac tgcctgctgc
     2101 atgtccacta gggaaactgt aacttgtttc aactactaag tgcgccaatg aaggacgctc
     2161 tcgcatgaaa aatagtttga tcaccgggtt gatgattcca gcaattacaa taaatccaga
     2221 aacgaaccaa aatgtttctg cccttttttt cttataccac atccataaag ccgcacaagc
     2281 aaataaaatg atgacggtaa gaggattacc gaatttagtc ataaagagtt ggtagctgtt
     2341 cagatcagga taagaagctc tgacaatgcc tgtaaaccac tgatcgaaac cagaaagcca
     2401 cgacagatgg aactttacga tccagcccaa aatgaaaaac aggacaagaa agaagctacc
     2461 agccacctga aatgatgttt tatatttcat acaattcctc ctttgaagca atgacttatt
     2521 atagcatttt tggtccagcg aaaaaaacgg taaagagata aaaccaatgt atgattcctc
     2581 tttgaataac caatattctt ataaaaaaaa taactgtaat cggaaagaca ttttttggta
     2641 atgcaaatga aatgttaggg aaataaaatc agtgtttttc gaaatccttt taaacaagaa
     2701 cgtagacgta ggaagaactt gaaaatgaac agaagttgtt cattacggta aattacaata
     2761 tgattacaaa aaacaacaaa gctcttaaat aacttttaat aaatattaag agaagttata
     2821 attattaata atagataaat tgaagttgga ggatagaaga tggaaaacat tgcaagaaaa
     2881 gaacggagac gtttagaaca gaccaaacgt tttcgaaaag caaaacgagg ggcagcaatc
     2941 gtgggaactg cgatggtagg ctgttctgtc gctgccccat tagtacagcc ggttcctgtt
     3001 caagcggatg aaacacctgt tcagttcggc gcaagaatca atacagcggc atttatcaca
     3061 gaaattgcta cttatgctca accaatcgct caagccaacg atctatatgc atcagtgatg
     3121 atcgcacaag cagttgtaga aagcggttgg ggcagtagtg cgttatctca agcaccttat
     3181 tataatcttt ttgggataaa aggcagctat caaggacaaa ccgtctacat ggatacgctt
     3241 gaatttttaa ataatcaatg ggtaaataaa aaggagcctt tccgacaata tccttctttt
     3301 gctgaatctt tcaatgacaa tgcctatgta cttagaaata cttcatttgg caacggttat
     3361 tactatgccg gtacttggaa gagcaacacg aaatcctata cagatgcgac agcttgtttg
     3421 acagggcgtt atgcgactga tcctggttac gcaggcaaat taaataatat catcgcaact
     3481 tacggattga cccaatatga tacaccagct agcggaaacg caggcggtgg agcgactaca
     3541 ggtggcaata atactggaag tgcaggtagt actggcggtt caagcaattc tggtaataca
     3601 aactcaggtt cagcaacttc gaccacttat actgtccaat ctggtgattc tgtttgggga
     3661 atctcaaata aattcggtat cacaatggat caattgatcc aatggaacaa catccaaaac
     3721 aatttcattt atccaggaca aaaactgaca atcaaaggta gccaggaaaa tggctcttcg
     3781 acgaataatt ccggtaacaa taccaactct tcaggaaatg ctggtacatc aaacggagga
     3841 caaacaactg gtgcgaaata cactgttcaa tctggtgatt ctgtttggaa gatctcaaat
     3901 gatcacggaa ttacgatggc tcaattgatt gaatggaaca atatcaagaa caatttcgtt
     3961 tatccaggac aacagctaat cgtatcaaat ggcggttctt ctaatgctgg agcaaatact
     4021 ggctcaaata ctgggaatac aagtggctct agttcgtcca gctcaagttc ttctaataca
     4081 gcttcaggaa cgaaatatac agttaaagcc ggagactctg tttggagcgt ctcaaataaa
     4141 tacggaatta cgatggctca gctaatccaa tggaacaata tccaaaataa ctttatctac
     4201 ccaggacaac aactgactgt ttcaaacagt ggatcaacaa acagcacgtc aggtaataca
     4261 ggttcgtcaa ccaatacaaa taacagcagt tcagttaata ctggcagcag ttcgtctcaa
     4321 ggcacttata ccgtgaaagc aggtgaatct gtttggagcg tggcaaataa aaacggcatc
     4381 acgatggatc agttaatcga atggaataat atcaaaaaca acttcattta tccaggacag
     4441 caattgatcg ttaaaggtgg aacttcggca aatacaaata caggttcaac tacttctgca
     4501 gcgaaaccaa atacaccgaa tacgtctgct acgacaagta catcatccac tggtaatacg
     4561 atgtataccg tgaaagcggg cgagtcagtt tggagcgtag cgaacaaaca tcatatcacg
     4621 atggatcagt taatcgaatg gaataatatc aaaaataatt tcatctatcc aggacagcaa
     4681 gtgattgtga agaaaggttc atctcaatca acaaaccaac aatcagttac tggttcgaaa
     4741 acctatactg tgaaatctgg agaatctgtt tggagtgttg ctgacagtca tggaattaca
     4801 atggctcaac taatcgaatg gaacaatatt aagaacaact ttatttatcc aggtcaaacg
     4861 ttgattgtag ctaaataaaa tagctgttcg aagaaataac gattgaattc cacaaaaata
     4921 tttgttacta taaggctact taaatatgac aacacttgaa aaggaagtag taaaaagact
     4981 gaagtttcag agagcgcatg gctggtgaga atgtgcacgg atgcttttga actcgccttt
     5041 gagttgccct tgctgaatac ccgagtaagc aagcggcggt gacggccgtt acaacgttcg
     5101 aggccttttc actaaggtaa aattaggtgg taccgcgtag attacgtcct ataaaaagag
     5161 caatcttttt ataggatttt tttatttctt ttgtgaagtt tgagaaaaca aagcaaaagg
     5221 aaataaaaat aaacagattc tgccggtttt ttcgttatat ttataataga aacaagtgtt
     5281 ttcaatagaa caggaggaca gcaaatgagt tacaatcata aagaaattga gaaaaaatgg
     5341 caaaaatact gggcaaaaac gaatactttc aacacccatg atgatcctga aaagccaaaa
     5401 ttttacgcac tggatatgtt tccctatcca tccggtcaag ggctacatgt agggcatcct
     5461 gaaggttata cggcaacgga tatcctttca cgtgtgaaaa gaagtcaagg atacaacgtg
     5521 ctgcatccaa tggggtggga tgcattcggt ctgcctgctg aacaatatgc gctagataca
     5581 ggaaacgatc cagctgagtt tacccaaaaa aatatcgaaa cattccgacg ccaaatcaat
     5641 tctttaggat ttagttatga ctggaatcgc gaagtgaata caactgatcc ggagtattat
     5701 aaatggacac aatggatctt tacgaagctt tatgaaaaag gtctagctta tgaagcagaa
     5761 gtagcggtaa actgggtacc tgaattagga acagttattt caaatgaaga agtgatcgat
     5821 gggaaaagtg aacgtggtgg ctatgatgta gtcagaaaac caatgcgcca atggatgctg
     5881 aaaatcactg cttatgctga tcgcttattg gatgatctag aattagttga ttggccagaa
     5941 agcattaaag aaatgcaaag aaattggatc ggtcgttcag taggagcaaa tattgaattt
     6001 aaagtggctg gtacggataa atcctatacc gtatttacga cacgaccaga tactctattc
     6061 ggtgcaactt actcggttct tgcacctgaa ctagatcttg ttagagaaat tacgacccct
     6121 gaacaaaaag ctgctgtaga agcttatatc gaagagacag ctaaaaaatc agatctaaag
     6181 agaacggatc ttgcaaaaga aaaaacagga gtatttactg gtgcttatgc aatcaatcca
     6241 gttaatggca aggaaatccc aatctggatc gccgattatg tattatcttc atacggcaca
     6301 ggagccatca tggctgttcc agcacatgat gaacgtgact ttgaattcgc tcaaactttc
     6361 ggactagaaa ttcttccagt tatcgaaggt ggagatgttg aaaaagctgc ttacacagaa
     6421 gatggtacgc acatcaactc tgaatttcta gacggcatga acaaacaaga agcaatcgat
     6481 aaaatgaatg aatggctaga agaaaatggc gtagggaaaa aagaagtcag ctatcgctta
     6541 cgtgactggc tattctcccg tcaacgttac tggggtgaac caatcccaat catccattgg
     6601 gaggacggaa cagtcacagc tgtcccagaa gaagagctac cattacggtt gcctaagaca
     6661 aaaaacatca agccaagcgg tactggagaa tctccattag caaatatcga ggagtgggtc
     6721 aatgtcgttg atcctgtaac aggtaaaaaa ggccgtcgcg aaacgaatac aatgccacaa
     6781 tgggcaggaa gctcatggta ttacctgcgt tatatcgacc ctcataacaa aaaagaacta
     6841 gcagattatg aaaagttgaa gcgttggcta ccagttgaca tctacattgg tggagcagaa
     6901 catgcggtac ttcatttact atatgcacga ttctggcata aattcttata cgatatcggt
     6961 gttgttccta caaaagaacc attccaaaaa ctatacaatc aaggaatgat cttaggcgaa
     7021 aacaacgaaa aaatgtcaaa atctcgtggt aatgtagtca atcctgatga tgtggtcgaa
     7081 acttacggtg cagatacact gcgcatgtac gaaatgttca tggggccatt agatgcttcg
     7141 attgcttgga gcgaaaatgg cttagaagga agccgtaaat tcttggatcg tgtatggcgt
     7201 ttgatcgttg atgagaacaa taaaatgcgt gaccgtatca ctacattgaa tgatggcaag
     7261 cttgataaag tgtatcatca aacagtgaaa aaagtgacag aagactatga aaatcttcat
     7321 ttcaatacag caatctccca attgatggtc tttatcaatg aagcctataa agtggacgct
     7381 ctgccttatg aatatatcga aggatttgtt caactactcg caccgattgc tcctcacatg
     7441 ggtgaagaac tatgggcaat ccttggaaat gatggtggta tttcttacgc accatggcca
     7501 acatacgatg aagcagcact tgttgaagac gaagtagaag ttgttttcca agtaaacggt
     7561 aaagttcgtg cgaaaagcaa tgttccccga gacttaggaa aagatgagct agaaaaagca
     7621 gcattagcca atgaaatagt gcaagaatat attgaaggca aaacagtcag aaaagttatt
     7681 gcggtaccaa acaagttagt caatatcgtt gctaattaat tggctctttg tcaataagga
     7741 ctgatgagtt gtgcaaaatc aaatctgggc cagaatgaac ttcattctgt ctcagatttt
     7801 ttgttatttc aaattgatta attgattgat caaaaaggtt ttgattttca tgttctcaaa
     7861 cacgttcaat gtctttttct tctgaatggt cttgtaagag atctctgtaa tcatcaaatt
     7921 tttgtctatt aatcttagta ttttttgatg tataaactac ctgaaattga gagtacatac
     7981 caaattcata aattttttat tagtgggact agtgattgta tggattttga cgcataatga
     8041 atgaagtatg agaaaaggag agacaaatgg atcaagaact atttattatc gaagtattat
     8101 cagctaaaca aaaaattatc gagaaagcga aaaaaagagc gaaaagaata atgttcaagc
     8161 ttttaagctt agtatttttc tctggtttgc tttttttcgg agctgtatcg ttcctatatc
     8221 gccatatgag tgcagtagtc cgttcagctg aagggatgca aaaattaaat aatttgtatc
     8281 acttttcgtg gggagttggc ggaataacaa ttgttgtgct tattgttttt cttctactta
     8341 tttggctccg ttaccatcaa ttgtcgcgtc atttcgatca atggcttagt caaagagcac
     8401 aacagttttt acataataaa ccaacttctc aagatgatga ctatctttat tttgagaaga
     8461 cttcgacgag tcaagctttt cggatacaaa aaagtgattg ccagttattg ctgagatcac
     8521 ttgatgattt acctatctac ttaggcgaaa gcaaatcaaa attcggaatg aacacattcc
     8581 atattttttt actagaaaac gaacccagat gggaaaaaag ccagtcttcg cctggtggat
     8641 acctgacaac taagatgagt acattagtca ctggtttttt cctcattctt ttagctggaa
     8701 caatctatta tgggagatac actcagccaa aaatgtcttc tcatataaat gctgcttctc
     8761 acatgacaat gaactcaact aacgatacgc atatacaaaa gacaaatgaa gggttgttag
     8821 ctgatcctaa gcaaaaggta aatttgacaa cacaaaagat aaatgatctc aaacttgata
     8881 cagagacgca ctctctctat atgacaacag atagtggtgc taattgggta tttgtgccga
     8941 ttgattcgca atggttgcgt tttggcgaat acaccctgac aacggggctg attcctgaag
     9001 gttattggat ggacaacaca tatgatattt ccgccgattt ctcatggttt atctattctt
     9061 ctggggatga tgtacgtctg ctgacttcaa gagataatgg taaaacatgg caaacgtcta
     9121 ttatcaccca atcagttcag ccgatacgtt atcgaaaagc ggtattttat ggcaacgatg
     9181 gtctatttat ctgtacatca gccagtggga tgtctgcaga aactttgtac atttacacta
     9241 ctatggatgg cggaatcact tggataaaaa gtggaagtac aatgatcaat cagccagtac
     9301 aaaatgtcag cttcgttaca acttctttag gttttgtttc gacacgtgaa agtttgtttt
     9361 atacaaacaa cggaggacgc tcattcaaag aatcagttct gtcgattccg gaaaactatc
     9421 agacaggggg gatagatctt tttcaatcac caaatgaagt tactcaaata agcgcgaata
     9481 cgttagagac aaaattttat ttgttgaaaa aaggaccaat cgatgcaggg aaaatgtttg
     9541 cctgcctctt ccgttcaact gataacgggg aaacatggca attcgttcag caactatcgc
     9601 aagtagaaca aggagattaa agaagagaga ttgtgagcct gacgttcagc accgagtcac
     9661 aagaaacaaa ttcaaaaaac agcttctcat atttgttgaa tagtgtaact cgtgattcgg
     9721 agttgacaag agcaacaaga agcgattttg aagtatagct tctcgtattt tacaagatcg
     9781 tgtcttattg ccgataggtt gtctcttgaa caccgtttat tcgtaaaaag ggattgtgac
     9841 aagtcttttg tcactactta ccgaacgaaa ctgaaggagc tatccaaaaa gtatcattag
     9901 tattattctt tgtgtttcta gtggcaccac tagtacattt aaaaaagcta tcgatcatgg
     9961 actaatcata catgatcgat agctttttag ataatgtttg ttacgctatc tgaaattgac
    10021 tattataaag actggcataa agcgttggtg attggagtag ttcagaatgt gttccttttt
    10081 cgacgatatc gccattcttc attacaagga tcatgtctgc attttcaatc gttgacaaac
    10141 ggtgtgcaat cacaaaactt gtcctatctt ttgtcacagc ttccattgct tgctgaatca
    10201 atgcttctgt tcgtgtatct acgctagaag ttgcttcatc taaaataacg acaggaggat
    10261 tcgctagaat gactcgagca attgtcaata attgttgctg tccttgagat aacgtgccgt
    10321 tttcgctaga aatgatcgta tcatatccgt ctggcagtgt tcgaataaag tgatcacatt
    10381 gtgcgatttc agccgcttcg ataatttgtt ctcttgtggc atccattttc ccgtaggcga
    10441 tattatcagc taccgttcct tcaaacagcc aggtattttg taaaaccatc ccaaaagaac
    10501 gacgtagttc tccacgtgaa agcttggtaa tatcaagacc gtcgaattcg atctttcctt
    10561 tattcagttc ataaaaacgc atcagcagat tgactaaagt cgtttttcct gcaccagttg
    10621 gaccaacgat cgctaccgtt tgttttggct ttactgaaaa atcgacttga ttcatcaata
    10681 ttttatctga tgtgtaacca aactgtacat ttttgaaagc tatttttcct ttcggttgtt
    10741 ctaaaagtct tgcatctgga aggtctggaa tttcatctgt ttcatctaat atctcaaata
    10801 tcctatcgat tgctgccatt gcggattgga tcgagttgat cacataagaa gcggtagaaa
    10861 ttggttcaga aatctgattg atgtactgaa gataagcttg taagaagccg atcgtcacac
    10921 cacctgatag aacaagcata gcaccgaata ccgcacttat gatgaaggcg agttgattaa
    10981 tgaaacgaat ggccggatag atagcacagt tcataaattg agcacgttta aaagcgttgt
    11041 attgcttttt atttacttcg tgtatagcag cttctgcatg agcttgctga ttgaatgttt
    11101 tgaccactag attaccagct aagatttctt ccattcgatt atttaaaaca cccagttcat
    11161 actgattctg ttcagaaatc tgtttgtttt tgttcgcaat cttagttgta acaattgaac
    11221 tgccaacgat cagcaataag acgagcaaag taagcttgct ttcgatataa aacagcatca
    11281 gaccagcagc agctattgtt acgacagaag taaaaaattg attgatacct gtcaataaca
    11341 cttgagaaag ttgattgaga ccggtagttg tccgactaat gatgtcacct acttgatgat
    11401 tgtcaaaaaa cgacatcggt aatgttttga atttttttgt gatttctttt cgcaccctca
    11461 atgtgatctt ttcgcttaac gctgccattg tccgttcctg aataaatgaa gtgatgctgc
    11521 ttaagactga acagccgatc agcaataaga cagggaacaa caatgcttgt ttgatatcac
    11581 tgaaagaaag ctgtccaata cctgtttttc ttatttctga taagagatca tcgatcccga
    11641 ttcccatgat caagggcatg gctacgacta ataggttccc gaataaactg catagaaaca
    11701 atccgtaaaa atatttatgt tcaggtttga tcattttcca aaaacgtatc aaagcacgcg
    11761 tgttcaacgt ttttttcttt tccatcgtat cccttctttc tatattcctt gtgattttgc
    11821 aaactcctga taagtttggc aattagacag tagttcttca tgagtacctt gtccaacgat
    11881 ttttccttca tctaaaacaa tgatgttatc tgcttgttgg attgtactga ggcgttgagc
    11941 aacaatcacg agagttttct ccgacatgtg tttatgaagg gctgctctta atgctgcatc
    12001 tgttttatag tctaaagcgg agaaactatc atcgaataca tagatctctg ctggtttgat
    12061 caacgctcga gcaatacata tccgctgttt ctggccccct gaataattag aaccgccttg
    12121 agcaacaaag ctgtccaatc cgtcaggaag ggtgtccact agttcttcta attgagcgat
    12181 cttgatcgct ttagacaact cttctgttga ggcatattca tttcccatct gcaagttgct
    12241 taaaattgtc ccgctgaata agaaagcctt ctgagggaca taactgatgt gggagcggat
    12301 ctgttcttga gtcatttgag taagaggaat accaccaagg gttacttttc ctatcgtagg
    12361 gtcactaagt ctcaagagaa gtttcgccaa actacttttt ccagatccgg tcccgccaac
    12421 gatcgctgtt gtttttcctt taggtattgt gaaatcaata tcttctagaa ctggatcaga
    12481 gatttcagta tatttaaatg tgacatgttc aaaagcagct gcatttgtct gatcaaaggt
    12541 tggttcagct actgttttga tcgtctcttc ctcaatttcg atctctgctt gcagtatttc
    12601 ttctatccgt cgtaatgagg caaacgattt agggataatg accaatacca tagctgcgat
    12661 catcaagtaa gcaagtgtca gcatagagta ttcgactaca gcagtcacga caccgatttg
    12721 taatgctcct tctgctacaa gatttccgcc aaaccacaaa atagaagcat aaacgattcc
    12781 catgagcaaa aaagcaattg gagtgaccca agcaaatatt cggttcactt tgatcatttg
    12841 attagcatag tcagcaaagc tcttattcgt tcgcttttct tcatatgatt gattatcaaa
    12901 ggcacggatc atattaatcc cagtaaagaa ttctctcaat gtgatggtga tccgatccat
    12961 ttttggctgg atactcaaag ataaaggtgc ccctcgtttc atcagtcgat aaacaatcca
    13021 tacataaaaa acaacagaga tcaatgtaac ccatgttagt aatagagaat aacgaaacat
    13081 catgagaatg gtgaaaacgc aaataaccgg tgctggtaag atcatttggc aaaataaaac
    13141 aatcatttgc tggacattat cgacatcatt cgtcattctt gtcaaaagag atccagaact
    13201 aaaacgactg atatccttca ttgacagctg ttggaattta cggaaatatt tctcccgcgt
    13261 ttggaatcca aacctagcgg aagccaatgc tgaaaagtaa ctagaggcaa ctgcagcgat
    13321 tgttcctaga aacgctacaa gcagcatttc aactccaatt ttttttataa ctgaggcttc
    13381 gccacttgct atacctgtgt cgatcagctt tgctactaat tttggtacac ctaatgtacc
    13441 ggcgatttct aaacagatca tcacaaaggt aaggagagcg agtcctttgt ttttctgtaa
    13501 gaatgttttt aagatagaca tattattcct ccttttttcc tgatactttt cgattctata
    13561 ccttcaagta acttgaaggt caatagaaaa aagaataaac tgaaaaagag gtgagaaaaa
    13621 atgaaaaaaa tgtatacgat tggccaagta tcggaactat ttgatttgcc aaaatcaacg
    13681 atccgctatt gggatgaaca aggattaatc cgttcaagta gacaagaaga gaatgattat
    13741 cgattgtttg atatcgatga tatttttatg ctttatgata tcgtttttta tagaaaatta
    13801 gatataccga tcaaacagat gaagaattta tatggaaaaa cattgacgga gttatatgag
    13861 acacttgatg aaacagaaag aagaatccat aaagaactgg tggtgatgaa acaaaaacaa
    13921 aaagagataa gggagcaaaa aaaacagttg aaattgatga tcgatacaaa cgaggaagaa
    13981 tttcctgtag aagagatccc ttttgactgt atgatctcaa cggagtttga agatattgtg
    14041 gaaatcaaaa aatttctacc taactattca agctttggga tgatgagtat gccagcttcc
    14101 agttcacaga cgatgtatgg attttttatc gaccctagtg aagtacattt atttactcaa
    14161 gacgtgattt gggagaaaaa agatacggct gtttatcgtc gattcttatt gaaatccgag
    14221 atgaaccatg cggaacgtaa taatatttta gaaatcagag aaagaatgta tgaaaaagga
    14281 tggaaaacag gagaagtgat cggccagtat ctattgacga atacggatga aaacaatatc
    14341 agaactgaat attatcatgc atggatagaa atgaaaaaat aggaaatatg tgtttaagat
    14401 aactcgaaaa tgatggttgt tttagttata aagaaaaatt ttctggttcg attacgggtt
    14461 taatgtgggg gattattagt cgtggataga tagaatttta gttttttaga ggaaatatgt
    14521 ctttaaaaat ggaatggttg caattcttag cgatatatac gatgtaattg tcctcttaaa
    14581 aaagtatgga aaaataattg tattgggtgt tatgtataat tcatggtgaa ctgagactaa
    14641 tgagaagaaa aattcatatt gttatgagtg agattgtatc agatgagaat gataaattaa
    14701 ataggtgcat aaaagcatta cacttaaaat acattgttgc ttacattatt acaatatgtt
    14761 aataaggcaa aaataaatag atggattgaa taaacatatt ttttaatcaa atagtcccta
    14821 ctgtgacttt cctcagctat cggtgatact accgatagca tttttttgct tataaaataa
    14881 ttataagtat ttagagattt ttcttctaac attcttcaaa caagaaaaat cacaaaggtt
    14941 attgttattt ttatatattc tttttaagaa tgattgtttt tttatagaaa ttttaaaaaa
    15001 taaaacacgt tattgatgga tagttagttt gttatttggt aatattatag aagttgactg
    15061 gtagtgattt atttttcgtc gttttataaa aatgttattt tttggaaaaa ttcacaaata
    15121 tgtaacgaat gt
//
LOCUS       DAZKLD010000067        15105 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.067, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000067 DAZKLD010000000
VERSION     DAZKLD010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15105)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15105)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15105
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.067"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(120..1370)
                     /locus_tag="ACG8KR_001654"
     CDS             complement(120..1370)
                     /locus_tag="ACG8KR_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFQ0201021.1"
                     /translation="MKLFVGIDVSSEKLDVCFLTDDNQLSILSEISVANDIEGASFIR
                     ETILEFNNSYHFDQIVIGMESTSMYSFHPSMFFHEDEKLKKLNTLVTIENPFRVKQFS
                     RMFDENKTDRNDALRIADFLRIQRFTTSPIKEEKYMALQRLTRTRYQLIKQLIRTKQH
                     FLENLTYKCNTLAREMRDESTSLFSATLISLMTEDFTLDELAELPLEAFCDLLQEKGK
                     GRFKQPEKIAKAIQRAITMSYRLGALAQESINVVLSVLVREIRALEKNIKELDKAIEQ
                     VIVVIPEYQCLTSIPGVGKVYAAGLIAEIGQIERFEDQTKLAKYAGLSWKVNQSGNYQ
                     SQNTPLTKQGNRYFRYYLVEAANSVKNYLPEYKAFYQSKYKEVPKHQHKRALVLTARK
                     FTRLVDTLLRKHQLYTPPRSVIEK"
     gene            1779..4151
                     /locus_tag="ACG8KR_001655"
     CDS             1779..4151
                     /locus_tag="ACG8KR_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArdC-like ssDNA-binding domain-containing
                     protein"
                     /protein_id="HFQ0201022.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNEQIEKMFEHQKMTNGQ
                     PMNDKRNGRSWFNQQERIENKVQSLREEIKQQEEYVEKLERQEELKEMGYNKYGGLDM
                     TIDNIPRIKEEIERFEKGESAFSAATIRKYQRKLETLEQLKEKSEKGKENILPEVQAI
                     IDSGRVTQWKKNPTIYFLKGYRKVALELNENGGFEESKKYQATSEEEKAAVRELLEEA
                     RPTDSMKEKENDHFYRVEFNENDPEKGLKSYGGEIVTFELIDQLKTLDNQYAAEAGYY
                     KFYFEEVKNGEVVDQIRMDLGDGVNSNQPIYLELAKLCEQVPEKEQKQSVKNEPVSKE
                     QMQSKETKTKQKGYQKRTAEEIKQEVKSLSQQALDAVKKHTHSPEDVKELLNFMSRFP
                     ERSFRNQLLIEEQFPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYEKGRGF
                     VRETEATPEDKKRIESGQIEVIKKPYYTIEKVFDITQTQLKPEDYPKIFPNRVFDFQL
                     DKKGQSELQAGIQAVANNIGIQIRDMTESEVYQRELGQARGAYVRNEFTGEEEIVMNT
                     RNTPTQHLATSIHELAHARMHKFSELDTATKELQAEMTSYIVCKHFGMDTSEKAIPYI
                     AAWTKNGQTLDDKEAAERGKIMNDVSRVANEFIQTISNEINQYREREPEIQPAEVKQE
                     HKNTLETPENKNIVPVGSLWIDKKDGKVMEEDTGRTLHLKDAAFIEDPQHNQVILKGK
                     SYGKEIQTALPSEEFALGNPFRFSLETVSEVAKRKNEIYYEQQLSQKERV"
     gene            4212..5672
                     /locus_tag="ACG8KR_001656"
     CDS             4212..5672
                     /locus_tag="ACG8KR_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HFQ0201023.1"
                     /translation="MTEKKMSLIDRCKQIDIVDFARNNGMAVVNKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDPKQQFKAATDFILKNEDKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPEWLIDYAEKEGLIAELKPKYERQNF
                     LVRDDRLDHAVAFLWKDPQTKETVGASYQGTFIDYERFGERGTYKHIDKNSTANHGFN
                     LKIGDPKQIKFFESSIDLLSYAALNRDQLNDTWLVSMEGLKHHVISHYFGEAVSELRK
                     KQAFPQSIEICVDNDRAGHIFYEKEQLMGAVDPFTNQKVRCERGIANDWQVPKEYKII
                     YEEVAKEEKVTPEAIMAIHKTENNLQLTNQLVSAHKVNAFFGQQLSVNDSIEAINLKD
                     ICREVAKELKVCERVDGTYDFDRFYQEKGDINAQILFSYKAEQYYKGYKNHEHEFVPE
                     VKKDWNDQLKHEIQQQEIRKQKRAMLFQQGRQQERE"
     gene            5683..7887
                     /gene="topB"
                     /locus_tag="ACG8KR_001657"
     CDS             5683..7887
                     /gene="topB"
                     /locus_tag="ACG8KR_001657"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFQ0201024.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFPGETVIT
                     YGFGHLVELDSPDMYDENWKQWSLEHLPIFPTHYHYHVPKDKKKQFKVVKQQLQSADT
                     IIIATDSDREGELIAWTIIQQVGADQGKTFKRLWINSLEKEAIYQGFQQLRDAKETYP
                     KFEEAQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FKKEPYFEGKSEVIAQNGAFDAKLDPNETQATQEAFEDYLKEKGVQLGKQPGMIRQVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EYAYLLDHLDEYKHFLRAEEISTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTEATYES
                     LTPLQQAIYEQVLKTTVAMFAEKYTYEETTILTQVQQLQLKAIGKVPIDLGWKKLFGK
                     ESDGKEKEEEPLLPKVTKGEMVTVDLQVLEKETKPPQPYTEGTLITAMKTAGKTVDSE
                     EAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGVLLCQAVEKEPLLT
                     SAEMTAKWESYLLKIGERKGTQTTFLANIQKFVSHLLEVVPGQIQSIDFGSTLQEVKA
                     ASEKQEAARHLGICPKCQEQEVLLYQKVAACTSEACDFKLWTTIAKKKLTATQLKEII
                     QNSRTSQPVKGLKGQKGSFEATIVLKEDFTTGFEFSEKKKTNFKKRTRRTTK"
     gene            7903..8052
                     /locus_tag="ACG8KR_001658"
     CDS             7903..8052
                     /locus_tag="ACG8KR_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0201025.1"
                     /translation="MRNTTHKQQEIENQKAKIRSEQEKLRASKRRLQQLKNQQRIQKE
                     IELDR"
     gene            8127..8720
                     /locus_tag="ACG8KR_001659"
     CDS             8127..8720
                     /locus_tag="ACG8KR_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HFQ0201026.1"
                     /translation="MQEKLKELLAIRDGNLSMKEARKLGIAATTVQRLVNQGKLIKVD
                     RGYYVEDGHGIDDLFWLQQRFSRGIFSHETTLDIYQLSTNMPKFIHLTFPHGYNVDRS
                     ITKEQQLQFHFVKKEVYELGKVEGTSFQGNPITMYDKERTLCDIWNPRYEIEYEMKLE
                     ALKEYMEDPLRDPSKLRDYMTKLHVEKEMKYHMQSLY"
     gene            8733..9632
                     /locus_tag="ACG8KR_001660"
     CDS             8733..9632
                     /locus_tag="ACG8KR_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HFQ0201027.1"
                     /translation="MTPTQFAAKTRNLAKRTGLNAQLVQRHFMMDRLIEQISESKYKN
                     DFILKGGFLLGSKYGIDRRTTIDIDTTFRNSKLTEEKLKSIFNDLTRKPTKEGIVFSI
                     QGMKETREADFYPGFQIKLVANLEKVRVPFKMDVTTGDSIYPEVDTHYHQLMFEDKKV
                     EIPAYPTAQIIAEKLSATFSFGTDNSRAKDYYDLFTIPKLENIDTKELYKSVRNTFTK
                     RGDTKPLSFYYEREMDKIIGNDYLEKQWEKYRSVNTFANSISFTDTVNEIDHLMKSVI
                     KIENQERKRQFKRSQQMNQEMER"
     gene            9635..10132
                     /gene="ssb"
                     /locus_tag="ACG8KR_001661"
     CDS             9635..10132
                     /gene="ssb"
                     /locus_tag="ACG8KR_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFQ0201028.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQNGNR
                     EADFINCVIWRKPAETMVTLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRAHADQSSTSPSTTTFEQRDTATPNNNGLNALQNPFGNPFGGQSIDISD
                     DDLPF"
     gene            10498..10758
                     /locus_tag="ACG8KR_001662"
     CDS             10498..10758
                     /locus_tag="ACG8KR_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0201029.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIASMYVRNPDHFTRSMGI"
     gene            11203..11409
                     /locus_tag="ACG8KR_001663"
     CDS             11203..11409
                     /locus_tag="ACG8KR_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HFQ0201030.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGQRAKCIKSYRLYQC
                     PTCGKKYTLSYGDYILIDEKGKKR"
     gene            11430..11660
                     /locus_tag="ACG8KR_001664"
     CDS             11430..11660
                     /locus_tag="ACG8KR_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0201031.1"
                     /translation="MTVKEVVQILVDKEVISEKVAEALLIPRYWTIETPLVEDAPTML
                     FEDLVKIAGYELHYQENMETYENEYWLTKLEN"
     gene            11676..12113
                     /locus_tag="ACG8KR_001665"
     CDS             11676..12113
                     /locus_tag="ACG8KR_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0201032.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYHETRVPKKHYKERLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNAKWFFQALLCLEVGVKPSTIKPSEYQALE
                     LTYGKFVETKKAKTVSSEWLDYFENINKYGAYYTMKKEDNENE"
     gene            12106..12813
                     /locus_tag="ACG8KR_001666"
     CDS             12106..12813
                     /locus_tag="ACG8KR_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0201033.1"
                     /translation="MNKVIYQLTTEDVSHFLTNKEHLNEVIRRIQKDFSCMEEMKYFI
                     QRVEEQLYLESETSQTKVTVSNNYLDVYKHRPETVKKGTELLRLETEKYIMNVYLERS
                     ELVENFVSCTICRKHYYSESEYYSIFESMELMEEEMEELNSKNATIDDYYLVFTRFLT
                     CGTESIVLMGMDYELAVPEAFLREYVEEVDNPGDDWYTTLTDFLDTYTYDDTEEIQNE
                     LDRKRMPYQRFERKKRQ"
     gene            12977..13177
                     /locus_tag="ACG8KR_001667"
     CDS             12977..13177
                     /locus_tag="ACG8KR_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0201034.1"
                     /translation="MNKIIPLLFVLCIGEILMRHGAAVHLNFVVFLGILVFCLIFWRF
                     VLALVSLLFKLIAAIFFIGLFR"
     gene            13193..13372
                     /locus_tag="ACG8KR_001668"
     CDS             13193..13372
                     /locus_tag="ACG8KR_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0201035.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESKIMCIALDLL
                     IAQEKAGFDIPALKK"
     gene            13697..13960
                     /locus_tag="ACG8KR_001669"
     CDS             13697..13960
                     /locus_tag="ACG8KR_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PbsX family transcriptional regulator"
                     /protein_id="HFQ0201036.1"
                     /translation="MEMLPIKKWGNSNGLRLPKYIMEYLGIHTEDKVKIIQEEVNGQK
                     RLIIEAANIENELTIEQLFENYTDERNHVTIQNLGEAVGNEKW"
     gene            13954..14304
                     /locus_tag="ACG8KR_001670"
     CDS             13954..14304
                     /locus_tag="ACG8KR_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFQ0201037.1"
                     /translation="MVRKPRQGDILLLNTAPRSGHEQTGKRPYIVLSHDIIADYSNVV
                     TVAPISSTTRNYPLYVNINSEYEMKTTGKVLLDQLTTIDYEARECIFLEKAHDSLVEE
                     LLMKVRTVFQKVSK"
     gene            complement(14328..14942)
                     /locus_tag="ACG8KR_001671"
     CDS             complement(14328..14942)
                     /locus_tag="ACG8KR_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFQ0201038.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDFVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVQLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSANAKDPQ
                     RRLVYKNIVEDLKKGVAIAKIAKDYNVTRQTVYRIKKDSMVNHE"
BASE COUNT         5468 a         2512 c         3082 g         4043 t
ORIGIN      
        1 caagatgaaa tttaagattt tctttattga gtaatatgtt ttgaagcgca cgaaactaga
       61 cccgatcaaa atcaaatttt tttcgggttg attcaaggat ttgcgtcaat aacggattgt
      121 tatttctcta tcacactcct tggtggcgta tagagttggt gtttacgtag tagcgtatcc
      181 accagacgcg taaattttct tgcggttagg acgagtgcac gtttgtgttg atgctttgga
      241 acctccttat atttactttg ataaaaagct ttgtattcag gaagataatt ttttacagag
      301 ttggcagctt caactaagta gtatctaaaa tatcgatttc cttgttttgt cagaggtgta
      361 ttttgcgact gatagtttcc agattggttc actttccaac ttaatccagc gtattttgcc
      421 aattttgtct gatcttcaaa tctttcgatt tgaccaattt cagcgattaa accggcagca
      481 tagactttac caacgcctgg aatactggtt aagcattgat attctggaat gacaacaatt
      541 acttgttcga tggctttatc taattctttg atattttttt caagtgctcg tatttctcgt
      601 acgagaacac ttaaaacaac attaattgat tcttgtgcaa gagcacctag gcggtaactc
      661 atggtaattg cacgttgaat tgctttagcg attttttctg gttgcttgaa acggcctttt
      721 cctttttctt gaagtaaatc acagaaagct tctaaaggaa gctcagcaag ttcatccaat
      781 gtgaaatctt ccgtcatgag tgaaatgagg gttgcactaa aaagactcgt cgattcgtct
      841 cgcatttctc gagcaagcgt attacatttg tacgttagat tttcaagaaa atgttgcttt
      901 gtacgaatca attgcttaat aagttgatag cgagtacgtg ttaaacgctg taaggccatg
      961 tatttctctt ctttgatagg ggaagttgtg aatcgttgaa tacgcaagaa atcagcgatt
     1021 cttaatgcat cgtttcgatc agttttattc tcatcaaaca tgcgactaaa ttgtttcacc
     1081 cgaaaaggat tttcaatcgt tacaagtgtg ttgagcttct ttagtttctc atcttcatga
     1141 aaaaacatgg aaggatggaa gctgtacatg gacgttgact ccatgccaat cacaatttga
     1201 tcaaagtggt agctgttatt gaattcaaga attgtttctc gtatgaatga agcaccttcg
     1261 atgtcattgg caacagagat ttcagataag atagacaatt gattgtcgtc tgttaaaaaa
     1321 caaacatcta gtttttcaga gctaacgtca ataccgacaa ataatttcat ggaagaagga
     1381 cctcctttcg atataaattt tggaaaatgc ttcgatactg tgggatttcc cagaaactca
     1441 ctgtatagcc acaacctcgc ctaatagaat acggtgataa aagagctaga agctgccttc
     1501 caaactacta tttgaaagtc tagtccatta tcgaaacagc taatgagttg gtagtgataa
     1561 cagtggttcg ggtaacagac taaacagggt agacaaacgc tacaggagga aatagcaaat
     1621 tcccgttgga tcctttccat acatccattg tactctgaga aatcacacaa tatcgaaaca
     1681 aattccgtta ggaatgtgtt attcaataaa ttttataatc tagaaacatt ttgaataaaa
     1741 actattagac aaatgatatt ttacgaggag gaaagaacat ggcagaagaa aaagaaccac
     1801 tagacaatag tcgtttggga aaatccaaaa gaaaactagt acgattgcaa aatgaattaa
     1861 acgaacaaat tgaaaagatg tttgaacacc aaaaaatgac gaacggtcaa ccaatgaacg
     1921 acaagaggaa tggccgttca tggtttaacc agcaagaacg aatcgaaaat aaagtccaaa
     1981 gtctgagaga agagatcaag caacaagaag aatacgtgga aaagcttgaa agacaagaag
     2041 agctaaagga aatgggctat aacaagtacg gcggactgga tatgacgatc gataatattc
     2101 caagaatcaa agaagaaatc gaacgctttg aaaaaggtga atcggctttt tcagccgcca
     2161 cgatccgaaa ataccaacgg aagttggaaa cattagaaca gctgaaagaa aaatcagaaa
     2221 aaggaaaaga aaatatcctc cctgaagtac aagcgatcat tgacagcgga cgagtgacgc
     2281 aatggaaaaa aaatccaacc atttattttt tgaaaggcta tcgaaaagtt gctttagaac
     2341 tgaatgaaaa cgggggattt gaggaatcaa aaaaatacca agccacgtcg gaagaggaaa
     2401 aagcagctgt tcgtgaatta cttgaagagg ctagaccaac tgattctatg aaggagaaag
     2461 aaaacgatca tttctatcga gtagaattca acgaaaatga tccagaaaaa ggattgaaaa
     2521 gttatggtgg ggagatcgta acattcgaat tgatcgatca actcaaaaca ttggataacc
     2581 aatacgccgc agaagcaggt tattataaat tctattttga ggaagtgaag aacggagaag
     2641 tagtcgatca gatccgaatg gatctagggg atggcgtcaa cagtaatcaa ccaatctatt
     2701 tagagttagc aaaattgtgc gaacaagtac ctgagaagga acaaaaacaa tcggtcaaaa
     2761 atgaacctgt ttcgaaagaa caaatgcagt cgaaagaaac aaaaacaaaa caaaagggct
     2821 atcaaaaacg aacggcagaa gagattaaac aagaagttaa atccctttcg caacaggcac
     2881 tggatgcggt gaaaaagcat acccattctc ctgaagacgt caaagaactt ttgaacttta
     2941 tgtctcgttt tccagaacga tcctttagaa atcaattatt gattgaggaa caatttccag
     3001 gtgcaacggc ttgtttaggt cgtgccgcgt tgaaaaaaga aggaatctat atcaaaaaag
     3061 gggaacaagg gaacaagatc tttgtcagaa aaacggtcaa agggttttat gagaaaggac
     3121 gtggctttgt tcgagaaaca gaagctacac cagaagataa aaaacgaatc gaaagtggtc
     3181 aaatcgaagt gatcaaaaaa ccttactata cgattgaaaa agtgtttgat atcacacaaa
     3241 cacagttaaa acctgaagac tatccgaaaa tctttccgaa ccgtgtcttt gattttcaat
     3301 tggataaaaa aggacagtca gaacttcaag ctgggattca agcggtagcc aacaatattg
     3361 gaatacaaat tcgagatatg acagaaagtg aagtctatca aagagaatta ggacaagcac
     3421 gtggggcgta tgttcgtaac gaatttactg gcgaagaaga aatcgtcatg aatactcgaa
     3481 atactcctac gcagcattta gctacaagta ttcatgaatt agcccatgca agaatgcaca
     3541 aattttcaga attagatacc gcaactaaag aattgcaggc agaaatgact tcttatattg
     3601 tgtgcaaaca ttttggaatg gacacgtcgg agaaagctat tccttatatt gcagcatgga
     3661 cgaagaatgg tcagacgttg gatgataagg aagctgcgga acgtgggaaa atcatgaatg
     3721 atgtcagccg tgtcgcaaat gaattcattc aaaccatttc taatgaaatc aatcaataca
     3781 gagaaagaga accagagatc caaccagcag aagtgaagca ggagcataaa aatactttag
     3841 aaacgccaga gaataagaat attgtgcccg ttggttcact ttggatcgac aagaaagacg
     3901 ggaaagtaat ggaagaagat acgggtcgaa cgttacattt gaaggatgcg gcgtttattg
     3961 aagatccgca gcataatcaa gtaattttaa aaggaaaaag ttatggaaaa gagatccaaa
     4021 cggcgttacc ttcagaagaa tttgcgctag gaaatccatt ccgattttca cttgagacag
     4081 tgtcagaagt ggcaaaacga aaaaatgaaa tctattatga acaacaattg tcacaaaaag
     4141 aacgtgtata aaacaagtaa aaaagactat ctgaaaaacg gatagtcttt tttcttttgg
     4201 gaggttttaa aatgaccgag aaaaaaatga gtcttattga tcgatgtaaa cagatcgata
     4261 tagtggattt tgctcgaaac aatggaatgg ctgtagtcaa taaaggacga gattatcgct
     4321 tagaagacca tgattcattt gtttttgatc gaagaaaaca acgtttttat tggaacagtc
     4381 aaaatattag tggcgacatt attgaattag ctaaactttt tttcatagac aaagagatcc
     4441 aagaccctaa gcaacaattt aaagcagcca ctgattttat tctaaagaat gaagacaaaa
     4501 cagaacgagt ggaaaatctt cattttgaaa cagaaaagta caaagatcac cccgttgatt
     4561 atcaaccact cacagaaaaa ggaaggaatt atctgaagga agaacgaaaa ctaccagagt
     4621 ggctgattga ttatgcagaa aaagaagggt tgattgccga actaaagcca aaatatgaac
     4681 ggcaaaattt cttagttcga gacgatcgtt tagatcatgc ggtcgccttt ttatggaaag
     4741 atccacagac aaaagagaca gtaggggcga gttatcaagg aacgttcatt gattatgaac
     4801 gttttggtga gagaggcaca tataagcata tagataaaaa ttcgacagcg aatcatggct
     4861 ttaacctaaa gatcggtgac cctaagcaga taaaattttt tgagagtagt attgatttac
     4921 ttagctatgc tgcactgaat cgtgatcagt taaacgatac gtggttagtc tctatggaag
     4981 ggttaaaaca ccatgtgatc agtcattatt ttggtgaggc agtatctgaa ttgagaaaga
     5041 aacaagcctt tcctcaatcc atcgagattt gtgtggataa tgaccgtgca ggacatattt
     5101 tctacgaaaa agaacaatta atgggcgcag tcgatccatt tacgaatcag aaagtacgtt
     5161 gtgaacgagg aatcgctaat gattggcaag tgccaaaaga atacaaaatc atttatgaag
     5221 aagtcgcaaa agaagaaaag gtcacaccag aagctattat ggccatacac aagacagaaa
     5281 acaatctgca attaacgaac caactggttt ccgctcacaa agtaaatgcg ttttttggcc
     5341 agcagctttc tgttaacgat tcaatagaag ccattaattt gaaagatatt tgtagagaag
     5401 tcgcaaaaga attaaaagtt tgtgaacgcg tggatggtac gtacgatttt gatcgttttt
     5461 atcaagaaaa aggggatatt aatgcgcaga ttcttttctc ttataaagca gaacaatact
     5521 acaaaggcta taaaaatcat gaacatgaat ttgtacctga agtaaaaaag gactggaacg
     5581 atcaattgaa gcatgagatc cagcaacaag aaatcagaaa gcaaaaacgt gccatgttgt
     5641 ttcaacaagg cagacaacaa gaaagggagt gaaatcgtca tgatgaaaac cgttatttta
     5701 gcagaaaagc cgtcacaagc gaaagcttat gccgatagct tctctaaagc cactcgaaaa
     5761 gacgggtatt ttgaaatcca agatcgattg tttcctggtg aaactgtgat cacgtatggt
     5821 tttggtcatt tagtcgaatt agattcgccc gatatgtacg atgaaaattg gaaacaatgg
     5881 tcattagaac atttgcctat tttccctaca cattatcact atcatgttcc taaagataag
     5941 aaaaagcaat tcaaggtggt gaaacagcaa cttcaatcgg ccgacaccat tattattgcg
     6001 acagatagcg atcgtgaagg ggaactgatc gcttggacga tcattcaaca agtaggcgcc
     6061 gatcaaggaa aaacctttaa aagactttgg atcaactcct tagaaaaaga agccatttat
     6121 cagggattcc agcagttacg agacgcaaaa gagacctatc caaaatttga agaagcacaa
     6181 gcacgtcaga tcgccgattg gttaattggg atgaacggta gcccactgta tagtttactc
     6241 ttgcagcaaa aaggcatacc aggaagcttt tccttaggca gagtccaaac cccaacgctt
     6301 tacatgattt atcaattgca ggaaaagatt agaaatttca agaaagaacc ttattttgaa
     6361 ggaaaatccg aagtaatcgc tcaaaatggc gcatttgatg cgaagcttga tccgaatgaa
     6421 acccaagcga cgcaagaagc atttgaagat tatttaaaag aaaagggggt tcaattggga
     6481 aaacagccag gcatgattcg ccaagttgaa accgagaaaa aaagcgcggc aagtccacgt
     6541 cttttttctc tatccagcct tcagtctaaa atgaatcagc tcatgaaagc tagtgccaaa
     6601 gacactttgg aagccatgca aggcttgtat gaagggaaat atctaagcta tccgcgaacg
     6661 gatacccctt acattacgga aggggaatat gcgtatctgt tggatcactt agacgaatac
     6721 aagcattttt tgagggctga agaaatttcg actcctattc atacaccgaa tagtaggtac
     6781 gtgaacaaca agaaagtcca ggaacattac gcaatcattc ctacaaaaac agttatgaca
     6841 gaagcgacgt atgaaagtct gacaccttta caacaagcga tttacgagca agttttaaaa
     6901 acgacagtgg cgatgtttgc ggaaaaatat acctatgaag aaacaacgat cttgacgcaa
     6961 gtccagcagc ttcagttaaa ggcaatcgga aaagtaccga tagatctagg ttggaagaag
     7021 ttatttggca aagagagcga cggaaaagag aaggaagaag agccgctact tcctaaagtg
     7081 acaaaaggag aaatggtgac ggtagatcta caagtccttg aaaaagaaac caagccacca
     7141 caaccttata ccgaaggtac gttgatcact gcgatgaaaa cagctgggaa aaccgtggat
     7201 agtgaagaag cgcaatccat attgaaagaa gtggaaggaa tcggtacaga agcgacacgg
     7261 gcgaatatta ttgaaacctt gaaacaaaag gaatatatca aggtagagaa aaataagctt
     7321 gtggtgacga ataaaggggt tttgctttgc caagctgtgg aaaaagagcc gctgcttacg
     7381 agtgccgaaa tgaccgccaa atgggaaagc tacttgttaa agattggaga acgaaaagga
     7441 acacaaacca cttttctagc caatatccaa aagtttgtct ctcacttatt ggaagtcgtc
     7501 ccaggacaaa tccaatccat tgattttgga tcgacgttac aagaagtgaa agcagccagt
     7561 gaaaagcaag aagccgcgcg tcatttagga atttgtccaa aatgccagga acaagaagtc
     7621 ttactctatc aaaaggtggc agcttgtacg tctgaagcct gtgattttaa attatggaca
     7681 acgattgcca aaaagaaact cacagcgaca cagctgaagg agattattca aaatagccga
     7741 acaagccaac cagtgaaagg gttaaaaggt cagaagggtt cgtttgaagc aacgattgtt
     7801 ttaaaagaag acttcactac aggctttgaa ttttctgaaa agaaaaaaac gaacttcaag
     7861 aaaagaacac gacgcacgac aaaataatgg agggttaatc ttatgcgtaa tacaacacac
     7921 aaacaacaag aaatcgaaaa tcaaaaagcc aaaattcgtt cagaacaaga gaaattacgt
     7981 gccagtaaaa gaagacttca gcaattaaag aaccagcaac ggattcaaaa agaaatcgaa
     8041 ttagatcgat agacattaac cttgttacaa atacactatc atgatatatt taatgtagtg
     8101 tatttgtaac aaaagggggg aagtaaatgc aagagaaact gaaagaattg ttggctattc
     8161 gcgatggaaa tttatctatg aaggaagcca gaaaattagg aatcgcagca accactgtac
     8221 aaagattggt gaatcaagga aaattgatca aagttgatcg tggttattat gtagaggatg
     8281 gtcatggaat agatgattta ttttggcttc agcaacgttt ttccagggga atcttctctc
     8341 atgaaacgac tttagatatt taccagcttt ctacgaatat gccgaaattc attcacttaa
     8401 cttttcccca tggatataac gttgatcgtt caattacaaa agagcagcag ttacaatttc
     8461 atttcgtgaa gaaagaagtt tatgaactag gaaaagtaga gggaacttcc tttcaaggaa
     8521 atcctattac tatgtatgat aaagagcgta cactttgtga tatttggaat ccgagatatg
     8581 aaattgaata tgaaatgaaa ttagaagcat taaaagagta catggaagac ccattgagag
     8641 atccaagtaa attaagagat tacatgacaa aactacatgt agaaaaagaa atgaaatacc
     8701 acatgcaaag cttatattga tggaggaaaa agatgacacc gacacaattt gcagcaaaaa
     8761 caagaaattt agcaaagagg acaggattaa atgcacagtt agtacaacgg cattttatga
     8821 tggatcgttt aattgaacaa atttctgaat ctaagtataa aaatgatttc attttgaaag
     8881 gtggcttttt attaggatca aaatatggaa tcgatagaag gacgacaatt gatatcgaca
     8941 cgacatttag aaatagcaaa ttaactgaag aaaaattgaa gagtattttt aatgatttaa
     9001 ctaggaaacc cacgaaagaa ggtattgttt tctctattca aggaatgaag gaaacaagag
     9061 aagccgattt ttatcctgga tttcaaatta aattggtggc taatttagag aaagtacgtg
     9121 tgccatttaa aatggacgtg acaacaggag attctattta tcctgaagta gatacacact
     9181 atcaccaatt aatgtttgaa gataaaaagg tggagattcc agcttatcca acagcacaaa
     9241 ttattgcaga aaagctaagt gctacgttca gctttggcac agataattcg agagcaaaag
     9301 attactacga cttatttacc attccgaaac tagaaaatat cgatacgaaa gagttataca
     9361 aaagtgttcg aaatacattt acaaaacgtg gggatacaaa accgttgtcc ttttattatg
     9421 aaagagaaat ggataagatc ataggaaatg actatttgga aaagcagtgg gaaaaatatc
     9481 gctcagttaa cacgtttgcg aattccattt catttacaga tacagtcaac gaaattgatc
     9541 acctaatgaa aagtgtgatc aaaattgaaa atcaagaaag aaaacggcag tttaaaagat
     9601 cacaacaaat gaatcaagaa atggagagat aaagatgatc aataatgtaa ctttagtagg
     9661 acgcttaacc aaagatccag atttacgtta taccgcgagt ggtactgcag tagcgacttt
     9721 taccctagct gtcaaccgaa atttcacgaa tcaaaacggc aatcgagaag ctgattttat
     9781 taactgtgtg atttggcgca aaccagcaga aacaatggtg acattagcta aaaaaggtag
     9841 cttgattggt gtagttgggc ggattcaaac gcggacttat gacaaccagc aaggacaacg
     9901 tgtgtatgtg acagaagttg tggccgataa tttccaacta ttagaaagca aagcagcgac
     9961 agaaagccgt gcgcacgctg atcaatcgtc caccagtcca agcaccacca cttttgaaca
    10021 acgggataca gcgacgccta ataacaatgg attgaacgct ttacagaatc catttgggaa
    10081 tccatttggt ggacaatcaa tcgatattag cgatgatgat ctaccgttct aagtagcatt
    10141 ttctattggt tgcatttgct acggtttatg ttataattta gtcaacaaaa agaattttca
    10201 atctataacc aaaaaccact agctggcggg ctagtggttt tcttttacgt ttctattata
    10261 agaattaacg ggtaggctta ctgtctttct tggcaaattt acaatcaagc catttgcaga
    10321 tatagtgcgc ggctacttct gccatgattg caaaaacaaa atcaaaaaga attttcaaac
    10381 ctataacctc ctccccttgc cagattaggc cggggtagca gacagcaaga ctattatagc
    10441 ataattgaaa tatccaaatg attggatttt tgctataatt ttagcgaggt gattgtgatg
    10501 aacaaatatg ttggtttgct tgataaaatc cgagtaataa aaacgcaacc gttattggtt
    10561 cgctttactt tacaaacgat tcatgaatcg attaattgtg tagttgcaga tattgaaatt
    10621 atcgataaat tgctaattat ggatgatgga aaatacaata ttgccgtcac aggtcacttc
    10681 aataaacgaa atcagctagt tattgctagc atgtatgtta gaaatcccga tcattttacg
    10741 cgatcgatgg gcatataaaa aaagaagagt tgcaatccta atgtaggact gcagctcttt
    10801 ttgttttaca tagaaacaag aaaaatgaaa aggccttttt aggaaggcgc gagcgccttc
    10861 tcaattgaaa aacctctctc actccaaccg ccaccgccgt tcgagaagaa aggaagtgaa
    10921 cgttgcacgt tcactaagat aaagggtctt gcaaagcaag acaaaataga aaaaacttcc
    10981 tctcaccaaa aggttcgttc ctgttcgtta tctctttatt gatttgccag accggcaaca
    11041 agttgccttt tatcaccaaa aagcgacaag gacaagggta aatggcacgc tgcgcgccct
    11101 tgtccttgtc gcttttcgtt gattcattct ggccaacaaa tcaataaaga aagaactaac
    11161 gaaaggaagt aaaacctttc ctaagaggag gcagaatcaa tcatgcgaaa gaaatttgat
    11221 tattggggtg ttcccttcag cgaattattc ccaaactatc atgcaccaca tactgtcgaa
    11281 tgcgattgtg gacaaagagc gaaatgtatc aaaagctatc gtttgtatca atgtccaaca
    11341 tgcggaaaaa aatacacctt gtcttatggc gattatattt tgattgatga gaaaggaaag
    11401 aaacgctgat gaaaacataa cataaattat tgactgtaaa agaagtcgtt caaattttgg
    11461 tggacaaaga agtcatttct gaaaaagtag cagaagcttt acttattccg cggtactgga
    11521 cgatagaaac ccctttagta gaggatgcac cgactatgct atttgaagac ctagtgaaga
    11581 ttgcaggata tgaactgcat tatcaagaaa acatggaaac ttatgaaaac gaatattggt
    11641 taaccaaatt agagaactaa aaaggagaaa aaaatatggc tcaaaattac tatgacgaat
    11701 ttgtcaaatt acctttggat aaaatggcac aaaaaatgga ggatatgaca ttcctatacc
    11761 atgagacacg tgttccaaaa aaacattata aggaaagatt gtcggtagct gtggaagaaa
    11821 tgattgaatc aggtgtcgaa atgaatctca ttgcgacgta ctatcgaaca ctggaagaac
    11881 tgaaaaaaca aaatgcaaaa tggtttttcc aagccttact atgcttggaa gtaggtgtca
    11941 aaccaagcac tattaaacct tctgaatatc aagctttaga actaacgtat gggaaatttg
    12001 tcgaaacgaa aaaagccaag actgtttcaa gtgaatggtt agattatttt gaaaatatca
    12061 ataagtatgg cgcctattac accatgaaaa aggaggacaa cgaaaatgaa taaggtgatt
    12121 tatcaactaa caacggaaga tgtgtcacac tttttaacaa acaaagaaca tttgaatgaa
    12181 gtaatcagac ggattcaaaa agactttagt tgtatggaag aaatgaagta ctttattcaa
    12241 agagtcgaag aacaattgta tttggaaagt gaaacgagtc aaacgaaggt cacagtcagc
    12301 aataattatt tagacgtgta taaacatcgt cctgaaacgg taaaaaaagg aacggagctt
    12361 ctacgactag aaacagaaaa atacatcatg aatgtttacc tagaaaggag tgaactggtc
    12421 gaaaactttg tcagctgcac gatttgcagg aaacactatt actcagaatc agaatattat
    12481 tcaatttttg agagcatgga attaatggaa gaagaaatgg aagagctaaa cagcaaaaat
    12541 gcgacgatag acgattatta tcttgtattt actagatttt tgacgtgtgg aacggaatct
    12601 atcgtgttaa tgggaatgga ttatgaactc gctgtacccg aagcattttt gagggaatat
    12661 gtagaagaag tagataaccc aggcgacgac tggtacacga ctttaactga ttttttagat
    12721 acctacactt acgacgatac agaagaaatc caaaatgaac ttgaccgtaa acgaatgcca
    12781 tatcaacgat ttgaaaggaa aaaaaggcaa taatcattta taccgaaaag tgagaatgtg
    12841 tgaaataaga acaaaaacct tgaaattaag ggaatggttc ttatttcgca ccaatcgctc
    12901 caaccgcccc cgccgcttag gtggtgcgaa ataagcgggc aaagcccgat aacctaagag
    12961 aaagaaggag atccatatga ataaaatcat tcctctgttg ttcgtactgt gtattggcga
    13021 gatacttatg cgtcatggtg cagctgtaca tttaaacttc gtagtattct taggaatact
    13081 agtattttgt ttgatttttt ggcgctttgt gttagccctt gtgagtctac tattcaagtt
    13141 gattgcagcc atatttttca tagggttatt tcgatgaaag gagagagtac taatgccaat
    13201 tgcaatagga aataaacggt tacccgtgac attagatgaa aagagacaaa aagaattgca
    13261 gcaactaaag cagaagtacg gaaaaagtga atctaagatt atgtgtattg cgttagatct
    13321 attgatcgcg caagaaaaag caggatttga tataccagca ctaaaaaagt gacgtcactt
    13381 ttcatttgaa gaaacaaaag tgatagcact taattgttcc tactatttag aatattttct
    13441 atgtcctttt tattccattt tgtatcaata actatgaaat ctttaaagta tccagatcaa
    13501 tttttttctt acctgattct aaagattttg ttttcttaaa catattatga tttcctttcg
    13561 actattttca tataaatgtt aacatagttt atggattcat ggaatgttaa tctaatttaa
    13621 tagaaaagga taattaagta tatacgtttt ttcttatgta tgttatacta tgtatataca
    13681 gaaagaggtg tctactatgg aaatgttacc tataaaaaaa tggggaaata gtaatggcct
    13741 tagattgcca aagtatatta tggaatatct tggaattcat acagaagaca aagtgaagat
    13801 aatacaagaa gaagtaaacg gtcagaagcg gctaattatc gaagcggcta atatagaaaa
    13861 tgaattaaca attgaacagt tatttgaaaa ttacactgat gaaagaaatc atgtgacgat
    13921 tcaaaattta ggtgaagcag taggtaatga aaaatggtga gaaaacctag acaaggcgat
    13981 attctattat tgaatacagc acctcgatct ggacacgaac agacaggtaa aagaccttat
    14041 attgttttaa gtcatgatat tattgcggat tatagtaacg ttgttacagt tgctccaatt
    14101 tctagtacaa ctagaaacta tccgctatat gtaaatataa actcagaata tgaaatgaag
    14161 acaacaggaa aagtattatt agatcaacta acgacaattg attatgaagc cagagagtgt
    14221 atttttttgg aaaaagcgca tgatagttta gttgaggagt tattgatgaa agttagaaca
    14281 gtttttcaaa aagtaagcaa ataaaataga aacagcagca tgatcaacta ttcgtgattg
    14341 accatgctgt ctttttttat tctatatacg gtttgtctcg taacattgta gtcttttgca
    14401 atcttagcga tcgcaacacc tttttttaga tcctcaacga tgtttttata aacaagacga
    14461 cgttggggat cttttgcatt ggcactgtat agcttaggac gtcctttata aacaccgttc
    14521 gcttttgcaa tcttaatgcc ttgtgcctga cgtcgtttag attcggtacg ttcttgttca
    14581 gcgatcatgg ccaaaatttg aatgatcaaa tctttaatga atttatccag gagaggattg
    14641 ccaattgctt cggccatgat cggtagactt gtgatgatca gctgcacatt tttcttcttc
    14701 aagtaattga ctgaagcgat gatctcgtcg taatttcttc caagtcgatc aatagcttcc
    14761 acaataaaaa tatcttgatc tcggacgaaa tccagtgctt cttgaaaaac aggtcgatga
    14821 gtaatcgttg cgccagattg tttctcaatg aaaattctct ctgcaccgaa ttttttcatt
    14881 tcttcaatct gccgttgttc attttgatcg atggatgaga cacgtatgta tgcaactctc
    14941 aaaattttta cctactttct gcaactattt aaaaaacacc ctaatcatac acgagaaaac
    15001 cagatttttc aatgtcgcgg tcgtgtctag ttagggtata ccttatatag acaaaaacta
    15061 atgatggagg aaatagtatg tgtcactttt ctttttaaat ggcaa
//
LOCUS       DAZKLD010000068        14815 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.068, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000068 DAZKLD010000000
VERSION     DAZKLD010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14815)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14815)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14815
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.068"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(296..1024)
                     /locus_tag="ACG8KR_001672"
     CDS             complement(296..1024)
                     /locus_tag="ACG8KR_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0201039.1"
                     /translation="MLRIAIVDDDCTIVEGIRHYLLKKVPDIIIDHCYTDGDEAFKNL
                     QNQRFDLLITDITMGKISGLELIESLQQTSNRYFTSIVISAHQNFQYAKKGIDLSIKS
                     YITKPIDFPYLVKLVQEEKMRKSAANQNSLTDDSFNESIQQVIDYVQNNYSEKLTLKD
                     VATQFHYHPAYLGRAFKKETGYSFHDYLLDFRIKRASALLKDTFLSIDEISQKVGIDH
                     VSYFSVLFKKTTGHSPSNYRKKLL"
     gene            complement(1002..2576)
                     /locus_tag="ACG8KR_001673"
     CDS             complement(1002..2576)
                     /locus_tag="ACG8KR_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HFQ0201040.1"
                     /translation="MKNDLSKVIMRFTYILIFFITLLACFTLFFSYINVIEKEKNQNL
                     QNIQQITHYMETYNRILSGIVTEIYQNYDFQENLNDYYLSSPAEYMEDILSKDRSPTY
                     LVDNLKKLVQDDEVKNISISLDYEKYLSAYAPTKNEKISFIRSLSFISEDMPDATLIL
                     DVSINALKKSLFPTHYNYSLSNGIDSVNLSEDISPSKWKKTQQIAIGNHTLQVYENPN
                     YSSQTLLGLAIMIFLLMSLFILAIHQLLNRSFRNYIQQYQEIITSLNHSSTGDYKSIN
                     IENKVGDLKEIAMHINQFYLDNATYQKTLYENNWLKQEAKYAALQYQIEPHFLFNNLE
                     FIRMTAILNDDEEISDSIYLLSKLYRNNTYQPDIIDLKEELINIDLSLQLHQRRYRHN
                     FDYQLIDHIDQLMLPKLSLQPFVENYIKHGFDSSKKENRLTVYATETTNHYVLKIVEN
                     GKGMDQDKLEHIQQKLNDDIPYSENIGISNSFRRIKHLYPGDTRFVIESQLHVGTTIT
                     IKIQKGGNMHVENCNC"
     gene            2805..3743
                     /locus_tag="ACG8KR_001674"
     CDS             2805..3743
                     /locus_tag="ACG8KR_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0201041.1"
                     /translation="MKLKKGLLGNLKKNRALFIMLLPGALWLFIFCYIPMFGVVIAFK
                     DFRISSRGFFTSLFNSPWVGWENFKYLFASQDAWIITRNTVVYNVVGILLSTFCTVSL
                     AIILSQMANKRLLKKMQTFMIFPNFLSWVVISYFVYAFLSTDKGLINILLSNFGVEPI
                     NWYSEPKYWPFIIVFTSLWKGVGYGSIIYYASIMGIDTSYYEAALVDGASKWQQIRNV
                     VLPHLRSIIVIMLILSVGSIFRADFGLFYQLPRNSGALYDVTNVLDTYVYRGLMNSGD
                     IGMSTAAGLYQSSVGFVLILITNKIVNKLDPSSALF"
     gene            3755..4666
                     /locus_tag="ACG8KR_001675"
     CDS             3755..4666
                     /locus_tag="ACG8KR_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0201042.1"
                     /translation="MISKLKRQMYFSLPVERFFTILLILFTLSCIFPFIFVIVISLTD
                     EKALQLNGYELLPAQWSTDAYQYLIQDGGQLLRSLGVTIMITVIGTLITVFMTGTYAY
                     VLSRASFPYRKFFTFYLFFTMLFAGGMVPSYLVMTKMLGLKNTIWALILPLAFSPYNV
                     IILRTFFKKSIPESIIESAKMDGCSEFRVFFQIVLPLAIPGVATIGLFSSLGYWNDWF
                     NALLYIDTNKLIPLQYLLMKIQNSMEFLANNNDITLAQQQAIQNSLPQESTRMAMVVV
                     ATLPIAIVYPFFQKYFVQGLTIGGVKE"
     gene            4690..6162
                     /locus_tag="ACG8KR_001676"
     CDS             4690..6162
                     /locus_tag="ACG8KR_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFQ0201043.1"
                     /translation="MKKNMQKYLIGGASVLTLGLLGACGSSNGEANNETGTTKVTWYQ
                     VGEEPKDFDEVMTKVNEKLKEEINVELDMRLIPDGDYQQKLGVMINSGEEYDICFVNG
                     TDYVNYGNKGAFISLNDHLDKELKDYAAELNEGFIEGGAINGETYGLPVNNDIATTEY
                     WTFNKDLVDQYQLDVENVEGVETLAPVLEKFAEKNTDTNITPIGMTRTSVCIPYDNII
                     GGGVPLAVAFEGDTQTVVNTYETPEMMTYLKAMHEFYKKGYILKDAATVTTDPFRIDS
                     KNWFLGRNSSGYQPLAQKKLEALAGHGLILKPAGKAFKTTGFARVAMHAVSASSKHPE
                     AALKLLNYVNTNQEVADLMVYGIEGKHYDRNEDGSITLTNSDDYYQGAWALVNTYLVS
                     KTSPITEESKKLEEESKKFQNEAPMSPILGFSFDASDFRTEIASVQNIVSQYEVLLSS
                     GTVDPEQYVPELLEKLEKAGLDKIQASAQEQYDEWRKSNK"
     gene            6213..6824
                     /locus_tag="ACG8KR_001677"
     CDS             6213..6824
                     /locus_tag="ACG8KR_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HFQ0201044.1"
                     /translation="MNENKWTKGFNWVFQLLLLNFTYFILICLGFGILGFFPATIALI
                     AGVVNRKKYGLQESVKFMSKIYKKNFVRYNLYCWSATLLLAGNITTIWLCWQQSAPLF
                     YLLSTLLSVITLFLVFAMLNWVVIKSLGLWMMGDRLIDYLLFTATMLPTVVMQGLVLG
                     IYFRILMILPVIFLFGGTAVTVILISKLTERALAKLPCQIQSH"
     gene            6854..8767
                     /locus_tag="ACG8KR_001678"
     CDS             6854..8767
                     /locus_tag="ACG8KR_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="HFQ0201045.1"
                     /translation="MKLTDKQVANIAFIQQLEIPLTQKDEEQIDLIFTDTKANVRQIK
                     ITKNEQQRICLSYSHEDLLYRGLFICLPRFLAQGGEMPYIEETSIIETSYSLDASRNG
                     VMNKETLKKFISYLALMGYNTLYIYTEDTYEVEDLPYFGYLRGAYTKEEVNEIVEFAS
                     GFGIEVVPSIQTLAHLTQFLKWYAQQELMDDQNTLLIGEEKVYAAIEKMIIACKEMYQ
                     TERIHLGMDEAYQAGLGRYLSLYGYCDRTTLMIQHMDRVLSIVDKYKMKPMIWSDFIY
                     KLLDETGKDRYYNPDFKLKALAAKGLPAGLTYVHWDYGSDEVSSYEKVIDNHLKFCDI
                     DHYVLASGAHIWGRLAPNHGKTEAIVSAAVKASKNKGVRSVMLTTWGDEGQETEHWHS
                     LIGALKLADYVYSEEEWNDFEEIYDRLLGTGSYQLMSELRYFDEVDTITNNNLQMASV
                     SKLLLWQDPLVGIYDFHVAAHNKIATRTIGTYYHELAQKLDMNKNWNPKQPILTTIRQ
                     RYILLAEILAQKAELGIYLQHCCLVEDLAGLMELANRCEALANRVNELASIHEIIWYQ
                     TYKTYGWEVLEQRYAALAHRLKTVARRIKNHLQGQDALEELLEERLPFCQYQVPLEIS
                     GFNYRQSSVSGYN"
     gene            8895..11876
                     /locus_tag="ACG8KR_001679"
     CDS             8895..11876
                     /locus_tag="ACG8KR_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HFQ0201046.1"
                     /translation="MLSDYYQNLSVISVNTEPLRNYYIPYASKEEAKQELEREKTSQF
                     IDLNGTWDFQYYPSVQQLPENFFQEAFTESYDRISVPGVWQTEGFDLPQYINVSYPIP
                     FDPPYVPNDTPCGVYRRQFQIEQTDARFYLNLEGADSNCFVWINRNFVGYDQVSHSTS
                     EFDVTKYLQVGVNEIQILVMKWCDGTYFEDQDKWRMSGLFRPVYLLQRPTMHLTSYQL
                     RTLTDPKKGSGTLMVSFQRFTPIEVSLPISYELFHPDGRLIAKGECYSEQQINLDVED
                     CLFWTAETPKLYTLLLTCNGEYFKEKVGFRALTIKNRKLLLNGTPIKLYGVNYHDVNQ
                     QGTPVMTREQLRADLLLMKKLNVNTVRTAHYPKSPDFYELADEIGLYVINEADLECHG
                     VVNLYDTEANYQLIANDPTFEKPIMDRVIRSILPYQNRSSIIMWSMGNESGYGVNFEK
                     AQALARKLDDSRLIHNERSIEPVIDQVNDFSNLDVISQMYPPLEDVHRHCQRSDTEKP
                     FFMCEYSHAMGNGPGDLKTYFDLIQQYDSFLGGCVWEWTDHAINRGTLETPSYLYGGD
                     FGEKYHDGNFCVDGLLTPDRQLTVGALEYRNIHSPIQLIAVDEELQEVTLSNITAFTA
                     GSAFNLSLEWYIDGKKTVYEVMNPFILEPGKSCSLPITVPTTIHGAHTVAVSLSVYKN
                     DELYGQQQLFLKKEKMRLPRTAHHNDAPQISVKGTELIIKGESFIYIYQLAKAAFTSV
                     KINQKELLERPTQFSIWRAPTDNDRNLVNKWKAAGYAEMSLEIRKKDWKFVDDHLLIE
                     TSYRGVIPGLQPIFTMHIQLKINGAGVINQKVSVEKNPIFPDFPRFGMDWPLNDTFSK
                     VSYCGYGPYESYPDKHYAAMFGIYEMEIEEFDQPYIKPQEYGNRWQCDWVSIKNHQGS
                     TMTVYGNSNFSALPYSQAQLTEKSHHYELIKENRTWFNLSYKQNGIGSHSCGPYLEDR
                     FRFMEDTFDWNIVITFKGENR"
     gene            11873..13534
                     /locus_tag="ACG8KR_001680"
     CDS             11873..13534
                     /locus_tag="ACG8KR_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein O-GlcNAcase"
                     /protein_id="HFQ0201047.1"
                     /translation="MKETKINQVKKKLSRYQEVVYRNHCLKWSKNKLNVRFNGLFYRF
                     EQLEEVWKPVTNFQSCPVQLINDPKETYDLCITHLYNQHLKNDGFYLKVTNDNQLTIE
                     SANLRGFQYAMEALLESFFSVGDQLLLPELILKHEPSVKIRGIIEGFYGIPWTHEMRL
                     SLFSYMKDNQLNTFMYAPKDDELLRKKWRELYDAQELDKFKELLSAAEASNIDFWYLI
                     SPGNDIDITCEEDIQVLLKKLEQLIELGVFQFGLLMDDIDYQLKGAAKRRFREPAFAH
                     AYLVNRVEEYLSTRLSNHELVICPTEYDNRHGSRYLATLSQEIPEQLPFFWTGPSTLA
                     ASISTEELQEMASVYQRPMLIWDNIPVNDYLEDKELLFMSPYENRTPNLSKERYQVTG
                     VVSNPMAQLEASKFTINSMANYLWNCERFDPLETWTSVVEKVVGSKLQPAYLTLTNAF
                     PNHYTSSLLSDEELLLAKDPEWVTKEMAALVQAVKWIQQEQHTSRLRTELEPWLQAIT
                     ESWTFWQEWQLKKDPKEAEEWLAKKKTHRIGRDLVTAHLEQIIKS"
     gene            13574..14506
                     /locus_tag="ACG8KR_001681"
     CDS             13574..14506
                     /locus_tag="ACG8KR_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005229489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HFQ0201048.1"
                     /translation="MVKRLISANYSDVKKMTSKELKQSIKASEGRVIVCETVANTLPQ
                     SGEITNAEVASAFGADLILLNGFDCYRPAVFGLPNVTIEKVFDLQQTIENPIPKLKEL
                     VGRPVGINLEPIAPKTNMMSEKVEISQGRASSVETIQKAEELGVDFICFTGNPGTGVT
                     NLEITKSVKMAKKYFSGLIIAGKMHSAGSEEPVVNKEAIISYAEAGADILLVPSVGSI
                     PGFLEQDLIEAVRLAKKYDLLTMSAIGTSQESARPETLRQLAIMNKSCGVDLQHIGDA
                     GYGGLAPAENIFETSVAIRGMRHTINCVSRSINR"
BASE COUNT         4815 a         2332 c         2978 g         4690 t
ORIGIN      
        1 atttaaataa ctacatccat ttattctatc ttctaaaata gcctttttca tttcagttaa
       61 atagttcagc gccatttttg cactcgactt gcatagtagt tttttccata ttcctcaacc
      121 tcagcggggg ttgaagacat aaggaaaatc ctatgcgtta tgctcaactg gatctgatta
      181 aatattctaa tgtatttttc tctaccatca ttaaatggtt aatagccaaa aatcaccact
      241 tttcaatggc tccttgttgt ataagtataa ctagaagatg aacacgtctg agaaattata
      301 gaagcttttt acggtaatta ctggggctgt gtccagtagt ttttttgaag agaacagaaa
      361 aatagctcac gtgatctatt cctacttttt gactaatttc atcaatagat aagaatgtgt
      421 ctttcagtag agcacttgct cttttgattc gaaagtctag caagtaatca tgaaaggaat
      481 aacctgtttc ttttttgaag gcccgtccta aataagcggg atggtaatgg aattgtgtag
      541 ctacatcttt gagtgtaagc ttttcactat aattgttttg aacgtaatca atcacttgtt
      601 gaattgactc attaaaagaa tcatcagtta gagagttttg attagctgct gatttacgca
      661 ttttttcttc ttgtactaac ttaactaaat aaggaaaatc aatgggtttc gttatgtaac
      721 ttttaataga taaatcaatc cccttttttg catactgaaa attttggtgg gcactaatga
      781 caatactagt aaagtagcga ttggatgttt gttgcaaaga ttcgattaat tcgagtccag
      841 aaattttccc catcgtaata tctgtgataa gtaaatcaaa gcgctggttt tgcaggtttt
      901 taaatgcttc gtctccgtct gtgtagcagt gatcgataat gatgtcgggg actttcttca
      961 aaagataatg ccttatgcct tctacaattg tacaatcatc gtcaacaatt gcaattctca
     1021 acatgcatat tccccccttt ttgtattttg atagtaatcg ttgttccgac gtgtagttgg
     1081 ctttctataa caaaacgtgt atctccagga tagagatgct ttatacgtcg aaaagaattt
     1141 gagatgccga tattctcact ataaggaata tcatcattaa gtttttgttg aatatgctcc
     1201 agcttgtctt ggtccattcc tttcccattt tcaacgattt ttaacacata atggttggtt
     1261 gtttcagttg catagacagt caaacgattt tccttttttg atgaatcgaa gccgtgttta
     1321 atataatttt caacaaatgg ctgtaaagaa agttttggta acattaattg atcaatatga
     1381 tcaatcagct gatagtcaaa gttatgtcga taacgacgtt gatgtagttg caaggagagg
     1441 tcgatattaa ttaattcctc tttcaaatca attatgtcgg gctgatatgt attattgcga
     1501 tacaacttgc tcaataaata tatactgtcg ctgatttctt cgtcatcatt caatatggca
     1561 gtcattcgaa taaattccaa attattaaat agaaaatgag gctcgatttg atattgaagt
     1621 gctgcatact ttgcttcttg cttcaaccaa ttattttcat aaagtgtttt ttgatatgta
     1681 gcattatcta aataaaactg attgatatgc atagcgattt ctttcaaatc accgactttg
     1741 ttttcgatat tgattgactt gtagtctcct gtagatgaat gatttaagga agtaatgatt
     1801 tcctgatact gctggatata gttacgaaaa cttctattta ataattgatg aatagcaaga
     1861 ataaacaaac tcattaataa gaaaatcatg attgccagac ctaataaggt ttgtgacgaa
     1921 taatttggat tttcatatac ttgtaatgtg tggttaccga tagctatctg ttgcgttttt
     1981 ttccatttag aaggcgagat gtcttcagat aaattgacgg aatcaatacc attagacaaa
     2041 gaataattgt aatgtgttgg aaagagactc ttttttagcg cgttgatcga aacgtctaga
     2101 atcaatgtag catcgggcat gtcctcacta atgaaactta atgaacgaat aaaagaaatt
     2161 ttttcgtttt ttgtaggtgc gtaggcactt aagtattttt cgtaatctaa tgagatagat
     2221 atatttttaa cttcatcatc ttgcacaagt ttttttaagt tatctactaa ataggtaggg
     2281 gagcgatcct ttgacaaaat atcttccatg tattcagcag gtgatgagag gtagtagtca
     2341 ttaaggtttt cttggaaatc ataattttga tagatctctg tcacgatacc ggagagaata
     2401 cgattatatg tttccatata gtgggtgatt tgctgaatgt tttgcaggtt ttgatttttt
     2461 tctttttcaa tcacattgat ataggaaaag aacaatgtga aacaggcaag taaagtaata
     2521 aaaaaaatca atatgtatgt aaaacgcata ataacttttg ataaatcgtt tttcattcaa
     2581 agtcctcatt ttgataatct ctaattttat gaatcattat agcacatatt acaagtgatg
     2641 taaacgcttt gcaaacaggg ctacttatga ttgtgaatac agtttccgtt taaaaactat
     2701 tctattgtag aggtttaata aactaaaaat aatgtataaa aaattaatgt aagcgattaa
     2761 ccaagtgggt ataatcaagg aaacgctgag agaggagaga caaaatgaag ctaaagaaag
     2821 ggttattagg aaatctaaaa aaaaaccgag cattgtttat tatgctttta ccaggtgcgt
     2881 tatggttatt tatattttgc tatataccaa tgtttggagt tgtaatcgct tttaaagatt
     2941 tcagaatttc atcaagggga ttctttacaa gtttgttcaa cagtccttgg gttggctggg
     3001 aaaatttcaa atatctcttt gcttcgcaag atgcttggat cattactcgc aatacggttg
     3061 tttataacgt tgtaggtatt ttgctaagta ctttttgtac agtgtcatta gcaatcattc
     3121 tgagccaaat ggcgaacaaa cggttattga aaaagatgca gacatttatg atttttccta
     3181 attttttatc atgggtagtc atcagttact ttgtttacgc atttttgagt acggataaag
     3241 gattaattaa tattttacta agcaattttg gtgtggaacc cataaattgg tatagtgaac
     3301 cgaagtactg gccttttatt attgttttta cgagtttatg gaaaggcgta ggctatggat
     3361 ccatcattta ttacgcgtca attatgggga tcgatactag ttattatgaa gctgctttag
     3421 ttgatggtgc cagtaaatgg caacagataa ggaatgtagt cttgccacat ttaagatcga
     3481 ttattgtcat tatgttgatt ttgtctgttg gtagtatttt tcgagctgat tttggattgt
     3541 tttatcaatt gccaagaaat tcgggggctc tttatgatgt gacgaatgta ttagatactt
     3601 atgtctaccg tggattaatg aatagcggag atatcggaat gtctacggca gcgggattat
     3661 atcagtcatc agttggcttt gtcttgattt tgattactaa taagatagtc aacaagttag
     3721 atcctagttc agcactattt taggaggaat caaaatgata tcaaaattaa aacggcagat
     3781 gtacttttcg ctgccagtcg aacggttttt cacaatatta ctaatcttat ttactcttag
     3841 ctgtattttt ccctttattt tcgtaatcgt catttcatta acagatgaaa aagcattaca
     3901 actcaacggt tacgaattat tgccggctca atggagcact gatgcatatc aatacttaat
     3961 acaagatggc ggacagttac ttcggtcatt aggcgttaca attatgatta cagtgattgg
     4021 aacgttgatt actgttttta tgacgggaac ctatgcttat gttttatcaa gagcctcttt
     4081 cccttaccgg aagttcttta ctttttatct tttttttacc atgttatttg cgggagggat
     4141 ggttccttct tacttagtga tgacgaagat gcttggctta aaaaatacaa tttgggcttt
     4201 gattctacct cttgctttta gcccatacaa tgtcattatt ttaaggacat tctttaagaa
     4261 gtcgattcca gaatcgatta tcgaatccgc taaaatggat ggctgtagtg agtttcgagt
     4321 ttttttccaa atcgttttgc ctttagcgat cccaggggtt gcaacaatag gtttattttc
     4381 aagcttaggt tattggaatg actggttcaa cgcattgctg tatattgaca ccaacaaatt
     4441 gattccattg caatatttgt taatgaagat tcaaaattca atggaattct tagcaaataa
     4501 taatgacatt acattagcgc agcaacaagc aattcaaaat agccttcctc aagaatcaac
     4561 acgaatggca atggtcgtag tagctacttt accgattgca attgtttatc cgttcttcca
     4621 aaaatacttt gttcaaggat tgacgatcgg tggagtgaaa gaataaaaaa gaataggaga
     4681 tgttaaaaga tgaaaaagaa catgcagaaa tatttgattg gtggtgcttc tgtactaacc
     4741 cttggcctat taggtgcttg tggtagcagt aatggcgaag caaataatga aacaggaacc
     4801 acaaaagtta cttggtatca ggttggagaa gaacccaaag attttgatga ggtcatgaca
     4861 aaagtcaacg aaaaattaaa ggaagaaatc aatgtagagt tggatatgcg gctgattccg
     4921 gacggggatt atcagcaaaa gcttggtgtg atgatcaatt caggagaaga atatgatatt
     4981 tgtttcgtca atggcacaga ttatgtgaat tatgggaaca aaggtgcttt cattagtctc
     5041 aacgatcatt tggataaaga gttgaaagat tatgcagctg aattgaatga aggatttatt
     5101 gaaggtgggg caattaatgg cgaaacttat ggattgccag tcaataacga tattgccaca
     5161 acggaatatt ggacctttaa taaagatttg gtagatcaat accaattgga tgtagagaat
     5221 gttgaaggtg tagaaacatt ggctccagta ttagaaaagt ttgcagaaaa aaatactgat
     5281 acgaatatta cgccaatcgg tatgacccgt accagtgtct gtattcctta tgacaatatt
     5341 atcggtggtg gtgtaccgct agcggttgca tttgaaggcg atacacaaac agttgttaat
     5401 acttatgaga ctccagaaat gatgacttat ttgaaggcta tgcatgagtt ttataaaaaa
     5461 ggatatattt tgaaagatgc agccactgtt acgacagatc catttcgaat tgatagtaaa
     5521 aactggtttt taggtcgtaa ctcaagtggt tatcaaccat tagcacaaaa aaaattagaa
     5581 gctctagcgg gacatggatt aattttaaaa ccagctggaa aagcttttaa aacgactgga
     5641 tttgcaagag tggcaatgca tgcggtatct gcttcaagta aacatccgga agcagccttg
     5701 aaattgttga attatgtgaa taccaatcaa gaagtagcgg atttaatggt ttatggaatt
     5761 gaaggaaaac actatgatcg caatgaagat ggttcgatta ctttaacaaa ctctgatgat
     5821 tattatcaag gcgcttgggc attggtaaat acttatctag tttcaaaaac ttcaccgatt
     5881 acagaagaaa gtaaaaaact agaagaagaa agtaaaaagt ttcaaaatga agcaccgatg
     5941 tctcctattt taggtttctc ttttgacgct tctgattttc gaacagaaat tgcaagtgta
     6001 caaaatatcg tgtcacaata tgaagtactt ttaagctcgg gaacagttga ccctgaacaa
     6061 tatgtaccag agttgttaga aaaactagaa aaagctggat tggataaaat tcaggcatct
     6121 gctcaagaac aatacgatga atggcgaaaa tctaacaaat aattttttac taaatgaaaa
     6181 gaatagacta agcagttagt ggggggattc aaatgaacga aaacaaatgg accaaaggat
     6241 ttaattgggt ttttcaattg ttgttactaa actttactta ttttatacta atttgtttag
     6301 gatttggaat tttaggtttt ttccctgcta cgatagcttt gattgctggc gttgtgaatc
     6361 gaaaaaaata tggcttgcaa gagtctgtga agttcatgag taaaatctat aagaaaaatt
     6421 ttgttagata taatctatat tgctggtcag caactttgtt gttggccggc aatattacta
     6481 cgatctggct ttgttggcag caaagcgcac cattgtttta tttattaagt acattattat
     6541 cggtaattac actgttccta gtctttgcaa tgttgaattg ggtcgtgatc aaaagtcttg
     6601 ggttgtggat gatgggagac cgactgattg actatttgct tttcacagca acgatgttgc
     6661 caacggtagt catgcaaggc ttggtgcttg gaatttattt ccgaatattg atgattttgc
     6721 ctgtgatttt tctgtttggc ggaacagctg taacggtaat attgattagt aaattaactg
     6781 aaagagcgtt agcaaagttg ccttgtcaga tacaatcgca ctaatttttt cacaaaaagg
     6841 agtatttctt tctatgaagt taactgataa acaagtagct aatatagcct ttatccagca
     6901 acttgagatt ccattgacac aaaaagatga agaacaaata gacttgattt ttactgacac
     6961 aaaggctaat gtgcgacaaa tcaagattac taaaaatgag cagcaacgaa tttgcttgtc
     7021 ttattcacat gaagacttgc tgtatcgagg attgtttatc tgccttccac gttttttagc
     7081 tcaaggagga gagatgccat acattgaaga aacctcgatc attgagacca gttattcatt
     7141 agatgcttct cgtaatggcg tgatgaataa agagactcta aaaaaattca tttcttactt
     7201 agcgttgatg gggtacaaca cattgtatat ttacacagaa gatacctatg aagtagaaga
     7261 ccttccatat tttggttacc ttcgcggagc gtatacaaaa gaagaagtga atgaaattgt
     7321 tgaatttgct tctggttttg gcatagaagt agttccatcg atccagaccc ttgctcactt
     7381 gacccaattt ttgaagtggt atgctcaaca agaactgatg gatgatcaaa atactctact
     7441 gatcggtgaa gaaaaggttt atgcggcaat cgaaaagatg attattgcct gcaaagaaat
     7501 gtatcagaca gagcgtattc atttgggaat ggatgaagcc tatcaagcag gtctgggacg
     7561 atatttaagc ctgtatgggt attgtgatcg gacaacttta atgattcaac atatggaccg
     7621 tgtgttatca attgttgata aatataagat gaaaccaatg atttggagtg atttcattta
     7681 taagctatta gatgaaaccg gtaaagatag gtattacaat cctgatttta aactcaaagc
     7741 attagcagct aaaggattac cagcaggatt aacctatgtt cattgggact atggttcaga
     7801 tgaagtttcc tcctatgaga aagtaattga taaccatttg aaattttgtg acattgatca
     7861 ttacgtttta gctagtggtg cccatatctg ggggagacta gccccgaatc atgggaaaac
     7921 agaagcaatt gtttctgcag ctgtaaaagc ttctaaaaat aaaggtgtac gttctgtgat
     7981 gttaactact tggggagatg aagggcagga aacagagcat tggcattcct taattggggc
     8041 attaaaatta gcagattatg tctattctga agaagagtgg aatgattttg aggaaatcta
     8101 cgatcgttta ttaggaacag gtagttatca attaatgtcc gaattacgat atttcgatga
     8161 agtggatacc attacaaata acaacttaca aatggcttcc gtgagtaaac tgctactttg
     8221 gcaagatccg ttagttggta tatatgactt tcatgtagcc gctcataaca aaattgccac
     8281 tagaacaatt ggtacttatt atcatgagtt agctcagaaa ttagatatga ataaaaattg
     8341 gaatccaaaa caaccaattt taacaactat tcgtcaacga tatatacttt tagctgaaat
     8401 tttagcacaa aaggcagaac taggcatcta tttacaacac tgctgtctgg tagaagacct
     8461 tgcaggatta atggaattag cgaatcggtg tgaagcacta gccaatcgag taaatgaatt
     8521 ggcatctatt catgaaataa tttggtatca aacatacaaa acctatggtt gggaagtatt
     8581 agaacaacga tatgctgcat tagctcatcg tctgaaaacc gttgcgcgtc gtattaaaaa
     8641 tcatcttcag gggcaagatg ccctagagga gcttttggaa gaacgattac cgttttgtca
     8701 atatcaagtg ccgcttgaaa tttctggctt taattatcgg caaagctcgg tgtcaggcta
     8761 caattaacga aggaatcttc ccgttgaacg gaccacttgc tcatctaatg ggaaagtggt
     8821 tcgttttcta ttcataagta gcttaccact aactctttta ttaagaagag aaaaaaagta
     8881 ggaagtgaga aaatatgtta tcggactatt atcaaaatct gtcagttata agtgtcaata
     8941 cagaaccatt acgtaattat tatattcctt atgcaagtaa agaagaagca aaacaagagt
     9001 tagaaaggga aaaaacgagc cagtttatcg atctcaatgg tacatgggat tttcagtatt
     9061 atccttctgt ccaacagtta ccggaaaatt tttttcaaga ggcctttact gaaagctatg
     9121 atagaatttc tgttcctgga gtttggcaaa cagagggatt tgacttacct cagtatatca
     9181 atgtatctta tccaattcct tttgatccgc cttatgtacc taatgatacc ccttgtggtg
     9241 tatatcggcg tcagtttcaa attgagcaaa cagatgcacg cttttactta aatctagaag
     9301 gagcagatag taattgtttt gtttggataa atagaaactt tgtaggctat gaccaagtga
     9361 gtcatagtac atctgaattt gatgtgacca agtatttaca ggttggggtt aatgaaattc
     9421 aaattctagt gatgaagtgg tgtgatggta cctactttga agatcaggat aagtggcgaa
     9481 tgagcggtct atttcgtcca gtgtatcttc ttcaacgacc aactatgcat ctgacaagtt
     9541 atcaactacg gacgttgact gatcctaaaa aaggaagcgg aacattaatg gtttcttttc
     9601 aacgatttac tcctattgaa gtatcgttac ctatttctta cgaattattc catcctgatg
     9661 gacgtctcat agcgaaaggg gagtgttact cggaacaaca aataaatttg gatgttgaag
     9721 attgcctttt ttggacagca gaaacgccta aattatatac attactttta acatgtaatg
     9781 gtgaatattt taaagaaaaa gttggctttc gagcactgac gatcaagaat cgtaagttat
     9841 tgctgaacgg aacacctatt aaattatatg gagtcaacta tcatgacgtg aatcagcaag
     9901 gcacgccagt gatgacccga gagcagttac gtgcagattt gttattaatg aaaaaattaa
     9961 atgtgaatac tgtacgaacg gcacattacc ctaaatcacc agatttttat gaattggcgg
    10021 atgagattgg tctgtatgta atcaatgaag cagatttgga gtgtcatggt gtggttaatc
    10081 tatatgatac tgaagcgaat tatcaactaa ttgctaatga tccgacattc gaaaagccga
    10141 ttatggaccg agtgatccgt tctattcttc cttatcaaaa tcgaagctct atcattatgt
    10201 ggtctatggg gaatgaatct ggttatggag taaattttga aaaagctcaa gctttagcac
    10261 ggaaattgga tgattcacgg ttgattcata atgaacgctc gattgaacca gttattgacc
    10321 aagtgaatga ttttagtaac ttggatgtta ttagtcaaat gtatccccca ctggaagatg
    10381 ttcatcgtca ttgtcaaaga agtgatactg aaaaaccttt ttttatgtgt gaatatagcc
    10441 acgctatggg caacggtcct ggagatctta aaacgtactt cgatttgatt caacagtatg
    10501 attcctttct tggcggttgt gtctgggagt ggactgatca tgcaatcaat agaggaactt
    10561 tagaaacgcc atcttattta tacggtggtg attttggtga aaaatatcac gacggcaatt
    10621 tttgcgtaga tggtttattg accccagatc ggcaattaac agtaggtgca ttggagtacc
    10681 gtaatattca ttcgcctatt caactgattg ctgtagatga ggagctgcaa gaagtaacct
    10741 tgagcaatat aactgctttt acagccggtt cagcatttaa cctttctctt gaatggtata
    10801 tagatggaaa aaaaacagtt tatgaagtaa tgaatccttt catacttgag cctggcaaaa
    10861 gttgttcatt gcccatcaca gtacctacaa cgattcatgg tgctcatacg gttgctgttt
    10921 cgctatctgt ttataaaaat gatgagcttt atggacagca acagctcttt cttaaaaaag
    10981 aaaaaatgag attgccaaga actgctcatc ataatgatgc accacaaatt tcagtaaaag
    11041 gcacagagct gataatcaaa ggagaatcct ttatttatat atatcaatta gcaaaagctg
    11101 cgttcacttc tgtaaagatc aatcaaaagg aacttttgga acgtcctact caattttcaa
    11161 tttggcgtgc accaacggat aatgatcgta acttagtaaa caaatggaaa gcagctggat
    11221 atgctgaaat gtctttagaa atcagaaaaa aagactggaa attcgtagat gatcatctcc
    11281 tgatcgaaac gtcttatcga ggagttattc caggattaca gccaattttt acaatgcaca
    11341 tacagttgaa aataaatggt gcaggcgtaa tcaaccaaaa agtatcagtg gagaaaaatc
    11401 cgatttttcc agatttccct cgctttggga tggactggcc gttgaatgat actttttcga
    11461 aggtttctta ttgtggctat gggccgtatg aaagttatcc tgataagcac tatgctgcga
    11521 tgtttggtat ttatgagatg gagattgagg aatttgatca gccatatatc aaaccacaag
    11581 aatatggtaa ccgctggcaa tgtgattggg tatcaatcaa aaatcatcaa ggatctacca
    11641 tgacggttta tggaaactct aatttttctg ctctgcctta tagtcaagct caattaaccg
    11701 aaaagtcaca ccattatgaa ttaataaaag aaaatagaac ttggttcaat ttaagttata
    11761 aacaaaatgg tatcggctca cacagttgcg gtccttattt agaagatcgt ttccgtttta
    11821 tggaagatac ttttgactgg aatatagtga ttactttcaa aggagaaaat agatgaaaga
    11881 aacaaagatc aatcaagtca aaaaaaaatt atctcgttat caagaagttg tttatcgaaa
    11941 ccattgctta aaatggtcaa aaaataagct gaatgttcgt tttaatggat tattttatcg
    12001 tttcgaacag ttagaagaag tctggaaacc ggtaacgaat ttccaatcat gtcctgtaca
    12061 gctcataaat gatccaaaag aaacatacga tttatgtata acacaccttt ataaccagca
    12121 tttgaaaaat gacggatttt atttaaaggt tactaatgac aatcaactta cgattgaatc
    12181 agcaaacctt cgagggtttc aatatgctat ggaagcctta ttagagagtt ttttttcggt
    12241 aggagatcaa ttattattac cggaattgat tctgaaacat gaaccatcag tgaaaattcg
    12301 aggaatcatt gaagggtttt atggtatccc ctggacacat gaaatgcgcc tctctctttt
    12361 ttcatatatg aaagataacc aattgaatac ttttatgtat gcccctaaag atgatgaatt
    12421 attacgtaaa aaatggcgtg aactctacga tgcgcaagaa ttagacaaat ttaaggagct
    12481 tttatctgct gctgaggcta gcaatatcga tttttggtac ttaattagtc ctggaaatga
    12541 tattgatatt acgtgtgaag aagacattca agttcttctg aaaaagttag aacaactgat
    12601 tgagcttggc gtatttcaat ttggtctatt aatggatgat attgactatc aattaaaagg
    12661 tgctgctaaa cgccgttttc gagagccagc attcgctcat gcctatttag tgaatcgagt
    12721 agaggaatat cttagcactc ggttgtccaa tcatgaatta gtcatttgtc ctacagaata
    12781 tgataatcgt catggttctc gctatttagc aacgttaagt caagaaatac cagaacaatt
    12841 accgttcttt tggactggcc caagcacgtt agcggcgtca atttctacag aagagctgca
    12901 ggaaatggct tctgtctatc aacggccgat gctgatttgg gataatattc cggtaaatga
    12961 ttatttagag gataaagaac tattgttcat gagtccatat gagaatcgaa caccgaatct
    13021 ttctaaggag agataccagg ttacaggagt agtttcgaat ccaatggctc aattagaagc
    13081 ttcaaagttt acgatcaata gtatggctaa ttacttatgg aactgtgagc gttttgatcc
    13141 tttggaaact tggacgtcag tcgttgaaaa agtagttgga agtaaactac aaccagctta
    13201 tcttacctta accaatgctt ttccaaatca ttacacaagc agtctgcttt cagatgaaga
    13261 attactgcta gccaaagacc cagaatgggt cacaaaagaa atggctgctt tagtccaagc
    13321 agtaaaatgg atccagcaag aacaacatac atctagattg agaacagaat tggagccttg
    13381 gttacaagca atcacagaaa gttggacttt ctggcaagaa tggcaattaa aaaaagatcc
    13441 aaaagaagca gaagaatggt tggctaagaa aaaaacacac cgcataggac gtgatttagt
    13501 aactgcccat ttagagcaaa ttatcaaaag ttagatatct taaaaaataa aaagcaagaa
    13561 aaaggagaac aaaatggtca agagattaat tagtgcaaac tacagtgatg tcaagaaaat
    13621 gacgtcaaaa gagttgaagc aatccatcaa agcgagtgaa ggacgggtga ttgtttgtga
    13681 aacggttgct aatactcttc cacaatctgg agaaattacg aatgcagaag ttgcgtcagc
    13741 atttggtgca gatttgattc ttttaaatgg tttcgattgt tatcgtcctg cagtttttgg
    13801 actaccaaat gtgacgattg agaaggtttt tgatctacag caaacgattg aaaacccgat
    13861 tcctaaatta aaagaactag tcggtcgtcc tgtgggaatt aatttagaac ccattgcccc
    13921 taaaacaaat atgatgagtg agaaagtaga aatcagccaa ggaagagcaa gctctgttga
    13981 aacgatacaa aaagcagaag aactaggagt cgattttatt tgctttaccg gtaatccagg
    14041 cactggagta accaatttag aaattactaa atccgtaaaa atggcaaaaa agtatttttc
    14101 gggattgatt attgctggga aaatgcatag cgccggctca gaagagccag ttgtgaataa
    14161 agaggcgatt atttcttatg cggaagcagg cgcagatatt ttactagttc cttcagtagg
    14221 gtctatccca ggctttttag agcaagacct aattgaagcg gttcggttgg caaaaaaata
    14281 tgatttgtta acgatgtctg caatcggtac tagtcaggaa agtgcccgac cagaaacatt
    14341 gcggcaatta gccattatga ataaaagttg tggtgttgat ttacaacata ttggagatgc
    14401 tggatacggg ggattggcac cagcagaaaa tattttcgaa acctcggttg caattagagg
    14461 aatgcgacat accatcaact gcgttagccg ctcgatcaat cgctaacaaa attaaataaa
    14521 aaatggttaa attgattatt taagtgggaa catacttgag atatcagcta acttctgtca
    14581 ctttaaaaaa gggactgtga cagaagtgaa aatagacaaa aaatatccga acaagagact
    14641 aggatagcag aaaaactatc gttttgcaac gaggaccaac tggaccgtag gagcaattct
    14701 cttattgttc ggatatttcg cttgttcaag gacttatgtc tgtagtgccc ttttttggta
    14761 ggaaaaatgg tagggaacca tatcattttt aatgaaaatc aagcatttca aaagt
//
LOCUS       DAZKLD010000069        14676 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.069, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000069 DAZKLD010000000
VERSION     DAZKLD010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14676)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14676)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14676
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.069"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(248..601)
                     /gene="tnpB"
                     /locus_tag="ACG8KR_001682"
     CDS             complement(248..601)
                     /gene="tnpB"
                     /locus_tag="ACG8KR_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFQ0201049.1"
                     /translation="MLYDLLQTKHIYIVCGKTDLRKGIDGLASLIQQEYQLELYEDAV
                     FLFCGNRQDRFKLLYWDGDGFLLCYKRIENGKLKWPRTKDEVRTLTNQQVRWLLEGLS
                     IDQPRAILPGKKGVF"
     gene            complement(591..785)
                     /locus_tag="ACG8KR_001683"
     CDS             complement(591..785)
                     /locus_tag="ACG8KR_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0201050.1"
                     /translation="MYKLNEIVPVQLGNEKSAPEKGKPAAAKLAMRIKRENGSEVLIY
                     NGINNYILQAILKELLSNAL"
     gene            complement(1011..2213)
                     /locus_tag="ACG8KR_001684"
     CDS             complement(1011..2213)
                     /locus_tag="ACG8KR_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFQ0201051.1"
                     /translation="MKTKYPCVYQDKNGRFSYLVELGTDQVTGKRIQKKGTKDSLGKR
                     FSSAREAHKEVMRIKNEYMVVNGFANYDLTYKEFMEQSYVPHYKASVQKSTWSSRECG
                     LIQITEFFGDKKLREINVRDCENYRIWLLNDSGYSQAYSSLMYGMFRKSLDYAVTLQF
                     LNENISKRTKAIPKGKSIVPYWTKEEFEEVLAKIYKDDFYEHMCFLMIWLYYMTGVRV
                     SEGLALNWNDVDFKRKKLRVHHTLEMTNQKDFIRKPYTKTESGLRIISLDDDTIEVLK
                     KWKVIQKNHGVEQFILSYTDLPLYRSTVQRIIQRYAKLAKVPLIQGKGLRHSHVSYLI
                     NEFNADILVVSQRLGHSSPEITLKHYAHLWSRNDEGIAEQMTGNIKFTCATKSKVANF
                     NGNQAVKI"
     gene            complement(2216..2410)
                     /locus_tag="ACG8KR_001685"
     CDS             complement(2216..2410)
                     /locus_tag="ACG8KR_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HFQ0201052.1"
                     /translation="MKNTICRKDLEALGFKTHQATTIIRQAKTRMVNQGYPYYNNKRV
                     GVVPRQVIEEIIGVPLSQEE"
     gene            complement(2457..3305)
                     /locus_tag="ACG8KR_001686"
     CDS             complement(2457..3305)
                     /locus_tag="ACG8KR_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rep protein"
                     /protein_id="HFQ0201053.1"
                     /translation="MQAYNQKIIETPTYIEIYDYEAIITPKRKTIENEVNLERKQKLA
                     WIYENDITLEKRKNYDELSAQGQYDSLKRKQKHYQSMRFEIARLVDTNFDNQTKFLTL
                     TFKENIQDIEYANNEFKKFIKRLNYDLYKTKKANLKYLATWEKQQRGAIHYHIILFDF
                     PFISFNRLTTIWGQGFVKINKIDVDSIENRGRYISKYFDKELELKEHKKKAFFKSRNL
                     KLPTVTKKMVKKPYDSTNQDILYTSEYIVRRPFFYNALALNELGSPEQVMDFQESTVH
                     YTKIKK"
     gene            complement(3510..3860)
                     /locus_tag="ACG8KR_001687"
     CDS             complement(3510..3860)
                     /locus_tag="ACG8KR_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002401157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0201054.1"
                     /translation="MPIKITDLSLPKETLEKEYQVVNVTRWQKEGEILGWSYECILPK
                     LRYEKISVKVKSEHPVITPEELTQQGLAIITFTNLVIVPWGRSNGQFVSYGLSATADT
                     ATLIPRKQGTTTQN"
     gene            4038..4901
                     /locus_tag="ACG8KR_001688"
     CDS             4038..4901
                     /locus_tag="ACG8KR_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002401156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFQ0201055.1"
                     /translation="MFGLVQPNKVKVGQRIKEIKDGMSLSFTELGNRLGLKKPTISSY
                     VQGYALAPKSVINQLSSISGKPVGWFYFGHVEEYIREYLFLIGQKKIVDDYPEIIEKI
                     KQEFYTGEFKNPAWENELGYPLEEFIQECFVEYSSEIMKDYVVQITKEVLEESKTLNK
                     LSSKAKEEAVTILSSGIIDYIEMSGEIKYGEKDRIAKMVKESIKTFDVKQDLTFNDSY
                     LIGKLINILGTDEDTELFLSSLSMIFTDKHFSTRFGGEELVEIFQSMRPALIKLYREK
                     TPDEFYDWFEK"
     gene            5525..6517
                     /locus_tag="ACG8KR_001689"
     CDS             5525..6517
                     /locus_tag="ACG8KR_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0201056.1"
                     /translation="MYWEEIELEKTLGPGGEIRAVQRDKKNISKWEKLKKQLRRDKWM
                     YLLLLPGIIMTFIFKYVPMYGAVIAFKDYNPMLGILESPWVGLDHFREFISSPNFTLL
                     LSNTLKISIYGLLLGFFPPIILALSLNQIINKKIKQKIQLILYAPNFISVVIIVGMLF
                     LFLSANGPINGLVEKVTGQPIMFMSNPAYFRTIYILSGIWQGIGWSSILYTATLAGVS
                     TDLYEAANIDGANILQKIWHIDLPALRPVMVISFILAAGGIMNVGYEKAYLMQTSMNI
                     PASEIISTYVYKVGLQSGDYAYSAAVGLFNMIINVVLLLIVNNITKRLNDGQGL"
     gene            6533..7417
                     /locus_tag="ACG8KR_001690"
     CDS             6533..7417
                     /locus_tag="ACG8KR_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016608569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0201057.1"
                     /translation="MNSALSKIDRQILWLNRVIVTFLVVITIVPLIYVLVASFMDPAT
                     LINKGISFNPKDWTIQGYQRVFADDSILRGFVNSLFYSFSFSLLTVFITMITAYPLSR
                     PNLAGKKIIMTFFVITMFVGGGLIPTYLLVKNLGMLDSVWAIIVPGAINVWNIILART
                     YFSALPKELSEAATIDGANDLQILFKVMLPLAKPIMFVLFLYAFVGQWNSYFDAMIYI
                     KDPDLQPLQLVLRNILIQNQPSQDMVGANTAMAEMKQIAELIKYATIVISSLPLIIMY
                     PFFQKYFDKGVLVGSVKG"
     gene            7466..9073
                     /locus_tag="ACG8KR_001691"
     CDS             7466..9073
                     /locus_tag="ACG8KR_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFQ0201058.1"
                     /translation="MKAKWMLPIVFTSVLILGACGGSKEKSKSSPDYELENVSFPLKE
                     KVSLKMMSQSAPLAPNDPNDKLIFQRLEEETNLHIDWTNYSSDFAEKRNLDIASGDLP
                     DAIFNAAAGDYDLLNWAESGVIVPIEDLIEDYMPNLKKIFDENPEYRQMSTAPDGHIY
                     SMPWIEELGEGKESIHTVNGMAWINKEWLDNLGLEMPQTTDQLMTVLEAFKTQDPNGN
                     GEADEIPFSFINNGGNEDLKFLMGAFGLGDNDDHLVVDNDGQVQFTANKEGYKEAIKY
                     FNEMNNKGLIDKEAFEQDWNTYVAKGKEHKYGVYFTWDKANITGADDSYDVLPVLAGP
                     DGTKHVTRTNGMGFSRDRFVITSANKNLELTARWIDKMYEPLQSVQNNWGTYGDEAQA
                     NIFELKDGMLKHLPLEGTAPGELRQKTEAGGPLAVLDSYYGTVTTMPDDAKWRLDLMH
                     DTYLPYITEDNIYPRVFLEKEETDRLAKIETDMWDYIYRKRAEWITGGKIDEEWEEYL
                     AELERVGLEEWIQIKQTGYDNFLKGAQ"
     gene            9074..10558
                     /locus_tag="ACG8KR_001692"
     CDS             9074..10558
                     /locus_tag="ACG8KR_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016621147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HFQ0201059.1"
                     /translation="MLVEETAILDKANRFIEMMRGKVNPQYRQNYHFSAPIGWINDPN
                     GFIYYKGHYHLFYQYYPYDSRWGPMHWGHARSSDLVHWEDLPVALAPDQPYDSGGCFS
                     GSAIERDGKLYLFYTGCQEIDGISHQHQCLAVSEDGIHFSKYSGNPVVAEKQLGENGL
                     IQEFRDPKVIVHNDGYYMVVATKSTQSTGRIVMFQSSDLYTWHFYSVLLEGTSEQGIM
                     WECPDLFHLDGRDVLIMSPIQIKKNGLEYHNISSTMACIGEVNWETGQMIVDNFHEMD
                     FGMDFYAPQTTRDPNGGRVMIAWMQMWDRTLPTHELGHLWAGSMSLPRELRVKKNRLV
                     QKPISTVYSHLVYQQGSENIAVTDTSVVFRNALSDSSYLHLVADLEKAEVFTIELLRS
                     KTDCLKLAYDTATQVFELDRQKLGRELTGAEEDPLNVRKVEVPLIDNQLVLEIFRDTS
                     SIEVFINGLEVMTATFYELEKARDILFHSIGESRIQAFETASVK"
     gene            10735..11727
                     /locus_tag="ACG8KR_001693"
     CDS             10735..11727
                     /locus_tag="ACG8KR_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005230454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0201060.1"
                     /translation="MKATMKDVAKLAGVGVGTVSRVLNNGSVKASTREKVEAAMTELN
                     YQPDFYARGLKMKHTNTVALILPTVWHPFFGEFAYHVETALERQNYKLYLCNSSGNSE
                     KEQEYIQMVSQNKVDGIIGITYSDIDEYISSNLPFVSIDRYFTEEVIYVTADNAAGGR
                     LAANELYRRGCQNVAYIGGYQETPNETKSRRKYFEIECRQLGISYKALDMLEPLDHLS
                     ERVTNFLLENPDIDGIFTINDPMALAVIDMLEKIGKNVPEDVQVIGFDGQKMTEGDPY
                     LVSTIAQPTKQMAEVAVAKLIAVINGETVEKRTVLPIEFGEGDTTKPLVRNDFA"
     gene            11779..12648
                     /locus_tag="ACG8KR_001694"
     CDS             11779..12648
                     /locus_tag="ACG8KR_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005472675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFQ0201061.1"
                     /translation="MLYGGIEAGGTKFVCGIGNEQLQVIERKSFPTTTPEETMEQVFD
                     FFDKYKGNIASIGVGAFGPIDIQIESRTYGYITNTPKLAWQNYHFVGALENALSVPIY
                     WTTDVNAACYGEYMAGYGKGKNSVLYYTVGTGVGGGAVVDGKIVAGFSHPEMGHMLLQ
                     RHNEDQFEGICPFHHDCLEGLASGPAIEKRFGRKAQTISFDHPYWTIEADYLAQCVYN
                     TTLLYAPEVIILGGGVMKQPQLLELIRQSFKEKMGSYVDTPTLEEYLLTPRLADNAGL
                     IGCLALAIKKLNH"
     gene            complement(13068..14633)
                     /gene="guaA"
                     /locus_tag="ACG8KR_001695"
     CDS             complement(13068..14633)
                     /gene="guaA"
                     /locus_tag="ACG8KR_001695"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HFQ0201062.1"
                     /translation="MSSPTRFDEEPKIIVLDYGSQYNQLITRRIREFGVFSELMSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDEKAFGIDEKIFDLGIPVLGICYGMQLITYKLGGK
                     VKPAKNREYGKAELEITSAEAALFQNTPEKQTVWMSHGDLVTQAPTDFDVVATSKDCP
                     IASIQNAERKMYGIQFHAEVRHSEYGNDLLRHFTLDVCQCTGDWTMDNFIDMEIAKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTCIFVDHVLLRKGEAEQVMESLGGK
                     FGLNIIKVDAKERFLSKLAGVSDPEEKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFKLIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGELTEEKLEIVRESDAILREEIANAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
BASE COUNT         4481 a         2510 c         3007 g         4678 t
ORIGIN      
        1 cgtccaaata atttgttggt taaataattg accttttcat tcaatagttg gatttcagtt
       61 gtaagattct gaatggtttg ttgttgtgct tgattggttt tatgggcttc ttgaagcaac
      121 tgaagcaacg tatctgtagt ttccaaaatt tttcaccacc ttgaatcgaa ctgattttgt
      181 tacagaatac cacaagaaac gcacaagaaa taggggttca acacattttt tgtgttaacc
      241 ccccagctta aaaaactcct ttttttcctg gcaaaatagc tcgaggttga tcgatactta
      301 atccttctaa caaccatcga acctgttggt tcgttaaggt tcgaacttcg tctttcgtcc
      361 gaggccattt taacttgccg ttttctatcc gcttataaca gagaagaaaa ccatcaccgt
      421 cccaatagag aagtttgaaa cggtcttgac ggttcccgca aaataaaaaa acggcatctt
      481 cgtaaagctc gagttgatac tcttgttgaa taagactggc aagaccatca attccttttc
      541 gcaaatctgt ttttccgcac acaatataaa tatgtttggt ttgaagtaaa tcataaagca
      601 ttggacaaca gttctttcaa aatagcttga agaatataat tgttaatacc gttatagatc
      661 aaaacttcag aaccattctc tcttttgatg cgcatcgcta acttggcagc cgcgggttta
      721 cctttttctg gtgcactctt ttcgtttcct aattggacag gaacaatctc atttaattta
      781 tacatagaaa acctccagac ttcttgatag ctcaagtata tcttgaggtg catttgttag
      841 taaggtactg ttctatgggg cgcttacgtt gaaaaacgtt gatttatcgg cattccccat
      901 ccatattata tttacgtctt attcccactc aatatgagta gcctcgtaaa gaagctatta
      961 tatcagtgct ttttcagcct attcctcatt ttggtaggga aatggtaggg ttagattttt
     1021 actgcttgat ttccattaaa gtttgcaact ttagattttg ttgcacaggt aaatttaatg
     1081 tttcctgtca tttgttctgc aattccttca tcattacggc tccatagatg agcgtagtgc
     1141 tttaaagtga tttcagggct agaatggcct aaccgttgcg acacgaccag aatgtctgcg
     1201 ttaaactcat tgattaggta gctaacatga gaatgacgta atccttttcc ttgtattagg
     1261 ggtaccttag ctagtttggc ataacgctga ataattcgtt gaacagttga acgatagagt
     1321 gggaggtcag tgtagctaag aataaattgt tctacaccat gatttttttg aataactttc
     1381 cactttttta gcacttcaat tgtatcatcg tccagagaaa tgatccgcaa gccgctttca
     1441 gtttttgtgt aaggtttccg aataaaatct ttttgatttg tcatttccaa ggtgtgatga
     1501 accctcaact ttttacgttt gaagtccacg tcattccaat taagtgctaa tccttcgctt
     1561 acacgaacac cagtcatgta atatagccag atcattaaaa aacacatatg ttcgtagaaa
     1621 tcatctttat aaatttttgc caaaacttct tcaaattctt cttttgtcca gtaaggaacg
     1681 attgattttc cttttggaat tgcttttgtt cttttggaaa tattctcatt cagaaattgc
     1741 agggtgaccg catagtctaa tgatttacga aacataccgt acataagtga actgtatgct
     1801 tgtgagtatc cgctatcgtt caataaccaa attcgataat tttcacaatc tcgcacattg
     1861 atttcacgaa gcttcttatc accgaaaaat tcagtaatct gaatcaatcc acactcacga
     1921 gaactccaag tacttttttg tacgctagct ttgtagtgtg gaacgtaaga ctgctccata
     1981 aattctttgt aagttaaatc ataattcgca aatccattta caaccatgta ttcatttttt
     2041 atacgcatga cttctttgtg tgcttcacga gctgatgaga atcgttttcc tagagaatct
     2101 tttgttcctt tcttctggat cctctttcca gtcacttgat ctgttcctaa ctcaactaga
     2161 taagaaaagc gaccattttt atcttgatac acacaagggt attttgtttt catagttatt
     2221 cctcctgtga aagaggaaca cctataattt cttcaattac ttgtcggggt actaccccaa
     2281 ctcttttatt attataatat ggatatccct gatttaccat tcttgttttt gcttgtcgga
     2341 ttattgtggt agcttgatgt gttttaaatc ctagagcctc taaatctttt cgacaaattg
     2401 tatttttcat tgtataaact cactttcaaa gaaaacacta tcattgggta atagtgttat
     2461 ttctttattt ttgtataatg aacggtactt tcttgaaaat ccatcacttg ttcaggactt
     2521 cccaactcat ttaaagctaa agcattgtaa aaaaatggac gtctaactat atattcgctt
     2581 gtatatagta tatcttgatt cgtggaatca tagggtttct ttaccatctt tttggtgaca
     2641 gttggtagtt tgagattccg ggacttgaaa aaagcttttt tcttatgctc tttcaattcc
     2701 agctctttat cgaaatattt gctgatatag cgcccgcgat tttcgatact gtctacatcg
     2761 atcttgttga tcttaacgaa accctgaccc caaatcgtag ttaaccggtt aaatgagata
     2821 aaggggaaat caaacaagat aatatggtag tggattgcac cgcgttgctg cttttcccac
     2881 gtggcgagat actttaaatt tgctttttta gttttataga ggtcataatt cagtcgtttg
     2941 ataaattttt taaattcatt atttgcatac tcaatgtctt gaatgttttc tttgaaagtg
     3001 agagttaaaa atttcgtttg attgtcaaaa ttcgtgtcta caaggcgggc aatctcaaat
     3061 cgcatacttt gataatgttt ctgtttgcgt tttaaactgt catattgtcc ttgagcggat
     3121 aattcatcat agttttttct tttttcaaga gtaatatcat tttcataaat ccaagctaat
     3181 ttttgttttc gttccagatt tacttcgttt tcgatcgttt tacgtttggg agtaatgatt
     3241 gcttcataat cataaatttc aatatacgtg ggagtttcaa tgattttctg attataagct
     3301 tgcaaaaatc atcactcctt gttgcgtatt cgattgtcaa agaaccagtc attttctcct
     3361 gatgttgatc tataaatcaa gttaaggggg aaggcctgcg tagcagccct tctagcctat
     3421 ggggtgttgg gcggtcttaa cggacagaaa cggctctgtg ggcgctgccc cgccgtttcg
     3481 tcctaaccac ccaacactcc atagtatagt tagttttgtg tagtggtacc ttgttttctg
     3541 ggaatcaaag ttgccgtatc agcggttgcc gataacccat aactgacaaa ttgtccattc
     3601 gagcgtcccc aaggtacaat caccaaattg gtaaatgtaa ttatggctaa accttgttgg
     3661 gtaagttctt ccggcgtgat cacaggatgt tcagatttga ctttgacact gattttttcg
     3721 tagcgaagtt ttggtaggat acattcgtag ctccaaccta gaatttcgcc ctccttttgc
     3781 cagcgagtga cattgacgac ttggtattct ttttcgagtg tttcttttgg taaactgaga
     3841 tcggtaattt ttattggcat cacaattctc cttttttata cgaaagttta tacgatttga
     3901 ttaaaaaata tagatggcca tctacgactt gtattatata cgagaactta tacgtagtca
     3961 agagattttc taaaaaaata tatacgattt tttatacgag tgttataatg aaaaaaatga
     4021 taaggaggaa tttgaatatg tttggtttag tacaacctaa taaagtgaag gtgggccaac
     4081 gaatcaaaga aataaaagac ggaatgtctc tctcttttac agaattaggg aatcgattag
     4141 gtttaaaaaa gccgacgatc agttcctacg ttcaaggcta tgcgttggct cctaagagtg
     4201 tgatcaacca attatctagt atcagcggaa aacctgtcgg ctggttttat tttgggcatg
     4261 tggaagaata tatccgagag tatttgtttc tcattggtca gaaaaaaata gtggacgatt
     4321 atcctgaaat cattgagaaa ataaagcaag aattttatac gggcgagttc aaaaatcctg
     4381 cttgggagaa tgagctaggc tatcctttgg aggaatttat ccaagaatgt tttgtagaat
     4441 attcgagtga aatcatgaag gactatgtcg tacagattac gaaagaggta cttgaagaat
     4501 ccaaaacgct aaacaaacta tcatcaaaag caaaagaaga agctgtcacg attctttcat
     4561 ctgggatcat tgactatatc gagatgagtg gtgagatcaa gtatggagag aaagatcgca
     4621 ttgcgaaaat ggtgaaagaa tcaataaaaa cctttgatgt gaaacaagac ctgacattta
     4681 atgattccta tttgattgga aagttgatca atattcttgg tacagatgaa gatacggagc
     4741 tgtttctttc ttctttatca atgatattta ctgataagca tttttccact cgatttggtg
     4801 gggaagaatt agtcgaaata tttcaatcga tgcgaccagc gttaatcaaa ctctatcggg
     4861 agaaaacacc agatgaattt tatgattggt ttgagaagta aaataagaaa agtggcttag
     4921 attacaagtt ggtgaaaaaa tttttttcag cataacatca gtaatctata aaggggttgt
     4981 tgattatact gtagaagtta agtactcaag agattttttg tacgtaaaca aagcaaaaaa
     5041 gggtgcagtt tcactagaaa atgtactctt ttttgtgatt tttcttagtt atagagtttt
     5101 tttgaaactt taaaaatgag aaaacatatt gattttaaag agtgtagaca tagcttcttt
     5161 tgctaaatag attaaactta ctggtgagtg gtcttgaaca tacttttaaa tcacatttta
     5221 ctgagtttta ttggatttta taattcattg gaaacctcgt acttaatctg tttttatcta
     5281 tcgactagaa tctgtatata ttccagctat caatgaggcc gcagaaaagg gtgcttaaaa
     5341 ataatctgtt gtttaaaata ttattatcag aatacttaga tacaaaattc tggcacaaag
     5401 taaaaaaaat attgacttaa aaaaatggat atggtaatct ataaacgaaa atagtggtaa
     5461 cgtttccaaa atgcatcttc gttaaatttt tattttttta aacgaaaatg gaaacgtttc
     5521 taaaatgtat tgggaggaga ttgaattgga aaagacattg ggaccaggtg gtgagataag
     5581 ggcggtacaa cgggataaaa aaaatatttc gaagtgggaa aaattaaaga aacagttgcg
     5641 gcgtgataag tggatgtact tgttgctctt accaggtatc atcatgacgt ttatctttaa
     5701 gtatgtgcca atgtacggtg ctgtgattgc atttaaagac tataatccta tgctaggaat
     5761 tttggaaagt ccttgggtag gactagatca ctttagggaa tttatcagtt caccaaattt
     5821 tacgcttttg ctgagcaaca cattaaaaat tagtatttat ggtttattgt taggtttttt
     5881 tccaccaatt attttagcac tgagcttgaa tcaaattatc aataaaaaaa taaaacagaa
     5941 aatccagttg attctatatg cgccgaattt tatctcagta gttattattg tggggatgct
     6001 gtttttgttc ctatccgcaa atggacctat taatgggtta gtagaaaagg ttacggggca
     6061 accaattatg ttcatgtcta atccagctta tttcagaacg atttatattt tatcgggaat
     6121 ttggcaagga atcggctggt catccattct ttacaccgca acgctggcgg gagtcagtac
     6181 cgatttatat gaggcagcaa atatcgatgg ggcaaatatt ttacaaaaga tttggcatat
     6241 cgatttgcct gcattgaggc ctgtgatggt gatcagcttc atcttggccg ccggcggtat
     6301 tatgaatgta ggatatgaaa aagcctactt gatgcaaact tcaatgaaca tccctgcctc
     6361 agaaatcatt tccacttatg tatacaaagt cggcttgcaa tcaggtgatt atgcctactc
     6421 tgctgctgtt ggtctgttca atatgattat taatgtcgtt ttattactga ttgtcaacaa
     6481 tataaccaag cggctaaacg atggtcaagg cttgtaatga aaggagaaaa ttatgaatag
     6541 tgctttatca aaaatcgacc gtcaaatctt atggttgaat cgagtgattg taactttttt
     6601 agtcgtcatc accattgttc cgctgattta tgtactggta gcttctttta tggacccagc
     6661 tactttgatc aataaaggta taagcttcaa tccgaaagat tggacaatac agggatatca
     6721 acgggtattt gcagatgatt ccattttacg cggttttgta aattcgttgt tttactcttt
     6781 tagcttcagc ttattgactg tatttattac gatgatcact gcgtatcctt tgtctcgacc
     6841 taacttggcg gggaaaaaga ttattatgac tttttttgtc attacaatgt tcgtgggtgg
     6901 tggcttgata cctacctatt tattggtaaa gaacttgggc atgttagata gtgtatgggc
     6961 gattatcgta ccaggtgcta taaatgtgtg gaatattatc ttagcccgta cctattttag
     7021 tgctttgcca aaagaacttt ctgaagctgc tactatcgat ggcgccaatg acttgcaaat
     7081 actgttcaaa gtcatgcttc cattagcgaa accaattatg ttcgtattgt ttttatatgc
     7141 ctttgttggg caatggaact catactttga tgccatgatt tacatcaaag atcccgactt
     7201 acagccgttg caactcgtat taagaaatat tttgatccaa aaccaacctt cacaagacat
     7261 ggtgggagcc aatactgcca tggcagaaat gaaacaaatt gctgagttga tcaaatacgc
     7321 aacgatcgtc atttctagtc tgccattaat cataatgtat cctttcttcc aaaaatattt
     7381 tgataaaggt gttttagttg gttctgttaa agggtaagta ttgttcacta tttgtatgaa
     7441 taaaaattat aaaggagatt ttaagatgaa agcaaaatgg atgctgccaa tcgtttttac
     7501 ttccgtacta attttggggg cttgtggagg ctcaaaagaa aagagtaaat caagccctga
     7561 ttatgaacta gaaaatgtct cttttccatt gaaagaaaaa gtatctttaa aaatgatgtc
     7621 tcaaagtgcc cctttggctc ccaatgatcc taatgacaaa ttgattttcc aacgattaga
     7681 agaagagacc aatctccata ttgattggac aaactacagt agtgattttg ccgaaaaaag
     7741 aaatttggat atcgctagtg gtgatttacc agatgccatc ttcaacgccg ctgcaggtga
     7801 ctatgattta ttaaactggg cggaaagtgg cgttattgta ccgattgaag atttgatcga
     7861 agattatatg ccaaatctga agaaaatttt tgatgaaaac cccgaatacc gacaaatgtc
     7921 tactgcacct gatggtcaca tttactctat gccttggatt gaagaattag gcgaaggaaa
     7981 agagtcgatt catacagtaa atgggatggc ttggatcaac aaggagtggt tggataattt
     8041 gggtcttgaa atgccgcaaa ccactgatca gttgatgact gtgttggagg ctttcaagac
     8101 gcaagatcca aatggtaatg gtgaagcaga tgaaattcct tttagcttta ttaacaatgg
     8161 aggaaacgaa gacttaaaat ttttaatggg ggcattcggt ctaggtgata atgatgacca
     8221 cttagtcgtt gataacgatg gtcaggtaca atttactgct aacaaagaag ggtataaaga
     8281 agccatcaaa tatttcaatg aaatgaataa taaaggtctt attgataaag aggctttcga
     8341 acaagattgg aatacgtatg ttgccaaagg gaaagaacat aagtatggcg tgtactttac
     8401 ttgggataaa gcaaatatca cgggtgcaga tgatagttat gacgtcttgc cagtcttagc
     8461 gggaccagat ggaacgaaac atgttactcg tacgaatggc atgggtttct caagagatcg
     8521 tttcgtaatt actagtgcga ataaaaactt ggaacttaca gctaggtgga tagataaaat
     8581 gtatgaaccg ttacagtctg tccaaaataa ctggggaacc tacggtgatg aagcacaggc
     8641 gaatattttt gaattaaagg atggtatgtt aaaacatttg cctttagagg ggacagcacc
     8701 gggggaacta agacaaaaaa ccgaagctgg aggaccatta gcagttcttg attcttatta
     8761 cggcacagtc actactatgc cagatgatgc gaagtggcgt ttagatttga tgcatgacac
     8821 gtatttgcca tatattacag aagacaatat ctatccacgt gtctttttag aaaaagaaga
     8881 aacagatcga ttagcaaaaa tcgaaacgga tatgtgggat tacatctatc gcaaaagagc
     8941 agagtggatc accggcggta aaatcgatga agaatgggaa gaataccttg ctgaattgga
     9001 acgtgtaggt ttggaagaat ggattcaaat aaaacaaact ggctatgata attttttgaa
     9061 gggagcacaa taagtgttag tagaagaaac ggcgattttg gataaagcaa atcgatttat
     9121 tgaaatgatg cgaggaaaag ttaaccctca gtatcgacaa aactatcatt tttccgcacc
     9181 catcgggtgg ataaatgacc ccaatgggtt tatatattac aaagggcatt accatctctt
     9241 ctatcaatac tatccttacg atagtcgatg ggggcccatg cattgggggc atgcgcgcag
     9301 tagtgatttg gtccattggg aagatctacc agtagcgtta gccccagatc aaccttatga
     9361 cagcggcggg tgtttctcag gaagtgcaat tgaaagagac ggaaaactat atttatttta
     9421 tacaggctgt caagaaattg atggtattag ccatcagcat caatgtttgg cggtctctga
     9481 agatggtatt cattttagca aatacagtgg taatcctgtg gtagcagaaa aacagctagg
     9541 tgaaaatggg ctaatacaag aatttcgtga tcctaaagta attgttcaca acgatggtta
     9601 ctatatggta gtagctacta aatctacaca gtctactggt agaatagtga tgtttcaatc
     9661 gtcagatttg tatacttggc acttttactc tgtgctgcta gaagggactt cagagcaagg
     9721 aataatgtgg gaatgtcctg atttattcca tttagacgga cgagatgtct taatcatgtc
     9781 tccgatacaa atcaagaaga acggactgga atatcataat atcagttcta ccatggcttg
     9841 tattggtgag gtaaactggg aaacaggtca gatgatagtt gataatttcc atgaaatgga
     9901 ttttggaatg gatttttatg caccacaaac aacaagagat cctaatggtg gacgggtgat
     9961 gattgcttgg atgcagatgt gggatcgtac attgcctaca catgagttag gccatctttg
    10021 ggcaggctct atgagtttgc cccgagagtt aagagtgaaa aaaaatcgat tggtacaaaa
    10081 acccatctca actgtatata gtcatttagt ttatcaacaa ggcagtgaaa atattgcagt
    10141 gactgacaca tcagtcgtat ttcgaaacgc gctgtctgat tcttcatatt tacatctagt
    10201 cgcagatttg gagaaagctg aagtttttac aattgaatta ctgcggtcta agacggattg
    10261 tctcaaacta gcctatgaca ctgctacaca ggtttttgaa ttggatcgtc agaaactagg
    10321 aagagagctt acaggggcgg aagaagaccc tctgaatgta cgaaaagttg aagtaccttt
    10381 gattgataat cagctcgttc ttgagatttt tagagatacc tcatcaattg aagtctttat
    10441 taatgggtta gaagttatga cggctacttt ctatgagctg gaaaaagctc gagatatctt
    10501 gtttcattcg atcggagaaa gtcgtattca agcttttgaa acagcgagtg tgaaataaat
    10561 gtttgattgc ttaggcgatg aatcaattat ttaataggtt ttgaaggata atgtttttag
    10621 aaaagaacag atagctagat ttcccatgga tttgctatta gtattttgaa aaaattagta
    10681 atttatgaca aaattcatta tactaaactt gtgcagaaat gtgagggatc aactatgaaa
    10741 gcaacaatga aagatgtcgc aaaattagca ggtgttggag taggaactgt ttcccgggta
    10801 ttgaataacg gttctgttaa agcttctact agagaaaaag ttgaagcagc aatgacagag
    10861 ttaaactatc aaccagattt ttatgcccgg gggttaaaga tgaagcatac gaacacggtt
    10921 gctttgattc tacccacggt atggcatcca ttttttggcg aatttgccta ccatgtagaa
    10981 acagcattag aaagacaaaa ctacaagctt tatttgtgta actccagtgg taatagtgaa
    11041 aaagaacaag aatatatcca gatggtcagc caaaataaag tagacggcat cattggaatt
    11101 acttattcag atatcgatga atacatttcg agtaatctgc cttttgttag tattgaccgt
    11161 tatttcactg aagaggtgat ttatgtgacc gctgacaatg cggccggtgg acgtttggct
    11221 gcaaatgaac tttatcgccg aggatgtcag aatgtagcat atatcggtgg gtaccaagaa
    11281 acaccgaatg aaacgaagag tcgtcgaaaa tattttgaaa tcgagtgccg tcaactgggg
    11341 atttcctata aagcgttaga tatgttagaa cctttagacc atctctcaga acgtgtaaca
    11401 aattttttgt tagagaatcc cgatatcgat gggattttta cgatcaacga tccaatggca
    11461 ttagctgtca ttgatatgct tgaaaaaata gggaaaaacg taccagagga tgtccaagtt
    11521 atcggatttg atggtcaaaa gatgacagaa ggagatcctt atctagtctc aacgattgcc
    11581 caacctacaa agcaaatggc ggaagtggca gtggcaaaat taattgctgt cattaatgga
    11641 gagacagtag aaaaacgtac cgtattgccg attgaatttg gcgaaggtga cacaacgaaa
    11701 cctttagtga gaaacgattt tgcttagtgc aaatacgttt ctttttttga aaatgagtga
    11761 atgaaaggtg tagataaaat gttatatggt ggaatagaag ccggtggaac aaagtttgtc
    11821 tgtgggattg gtaatgaaca attacaagtg atagaacgga aaagttttcc taccacaaca
    11881 ccagaagaaa cgatggagca agtgtttgat ttctttgata agtacaaagg aaatatcgca
    11941 tcaattggtg taggagcatt tgggcctatt gatattcaaa tcgaatcaag aacttacgga
    12001 tatatcacca acacaccgaa gttagcttgg caaaattatc attttgtagg agctttggaa
    12061 aacgccttgt cggttcccat ctattggact accgatgtaa atgctgcttg ttatggagaa
    12121 tatatggcag ggtatggaaa aggtaaaaat agtgttctgt attacaccgt tggtacaggt
    12181 gttggtggtg gtgctgtagt ggatgggaaa atagtcgcag gttttagtca tcctgaaatg
    12241 ggacatatgc tcttacaacg tcataatgaa gatcaatttg aaggaatctg tccttttcat
    12301 catgattgtt tggaagggtt agcatcagga cctgcgattg aaaaacgctt tggaagaaaa
    12361 gcccagacta tctcatttga tcatccttat tggacgattg aggcagatta tcttgctcag
    12421 tgtgtgtata acacaacgtt actatatgca ccggaagtaa taattttagg cgggggtgta
    12481 atgaaacagc cgcaactact cgagttgatt cgccaatcgt ttaaagaaaa aatgggttcc
    12541 tacgtggata cgcctactct cgaagaatat ctactaactc caaggcttgc tgacaatgcg
    12601 ggcttgattg gctgcctagc tttagcaata aagaaactca atcattagaa tagtatttat
    12661 tagaactatt cataacatac cggaagttta aaagggtatt ctgaaactta aatagtctca
    12721 attcattctt ctttcttgag aatctgagta gagactttta ttctgtttca aattaatata
    12781 attacgaaat gatattgagt acaagaaagt tctttttacc agatctttta aatcgaaaaa
    12841 tctgatatat atatttatag tactaaaaat ctgattagat aaaaagctaa ttcaatagga
    12901 aaataaaggt agtgaataaa agcaaagccc ttgttagtag ttaatctaac aagggctttg
    12961 gtagggaaaa tggtaggaaa ccatcttatt ttttacgaaa atcaggaact ttagtaattg
    13021 aataatgttg atttataggt attcctcatc catattatgt tggctcttta ttcccactcc
    13081 actgttgcag gtggtttgct ggtaatatca tacacgatac ggttaacgtg tgcgacttca
    13141 tttacgatac ggacactgat tttttgcaag acttcccaag ggatacgggc aaagtcagcg
    13201 gtcatgccgt caatagaagt gacagcacgg atacctacag tgtagtcata cgtacggcca
    13261 tctcccataa ccccaacaga gcgaatgcct ggtaggacgg tgaagtattg ccagatgtcg
    13321 cgatctaaac cggcattggc gatttcttcg cgcaagatag cgtctgattc acggacgatt
    13381 tctaattttt cttctgtcaa ttcaccaagg acacgaatac ctaagcctgg tccagggaat
    13441 ggttggcgcc aaacgatgct gtcaggcatg cctaattggg tacctaattc acgtacttcg
    13501 tctttgaaca atgtgttcaa tggctcgatc agcttgaatt gcatgtcttc aggtaatcca
    13561 cctacattgt gatgtgattt gatggtttgg gcagtttcag ttcctgattc gatcacatcg
    13621 gtataaaggg ttccttgtgc aaggaaagaa acgccttctt caccggcaag tttggttgct
    13681 tcatcatcga agacatatac aaattcattt ccaatgattt tacgtttttc ttctggatca
    13741 gacacgccag caagttttga taagaaacgt tctttggcat cgacttttat aatgttcaag
    13801 ccaaattttc cgccaagact ttccataact tgttctgctt cacctttacg caacaatacg
    13861 tgatcaacga agatacatgt taattgatcg ccgatcgctt tttgtaaaag aacaccaaca
    13921 acagaggaat ctactccgcc tgaaaggcct aataaaactt ttttgtcacc gacttgttca
    13981 cgaatttttg cgatctccat gtcgatgaag ttatccattg tccagtcacc agtacattgg
    14041 cagacatcta gtgtaaaatg gcgcaacaag tcatttccat attcggaatg gcgaacttct
    14101 gcatggaatt ggattccgta cattttgcgt tctgcatttt ggatcgatgc gatggggcaa
    14161 tctttgctgg tagcaacgac atcgaaatca gtcggtgctt gggtaactag gtcaccatga
    14221 ctcatccaaa cggtttgttt ttcaggagtg ttttggaaaa gggccgcttc tgcagaagtg
    14281 atttccaatt ctgcttttcc gtattcacgg tttttggctg gtttaacttt tccgcctaat
    14341 ttataagtaa tcaactgcat accgtaacag attcctaaaa ctgggatccc caaatcaaaa
    14401 atcttttcat caataccaaa ggccttctca tcataaacac tgtttggtcc gccagaaagg
    14461 atgattcctt tagggttcat tgcctttact tcttctgcgg taatgcggtg gctcatcaac
    14521 tctgagaaaa caccgaactc acgaatacgg cgagtgatca gttggttata ctgactaccg
    14581 taatcaagta cgataatttt tggctcttcg tcaaatcgtg ttggtgaaga caaatttgaa
    14641 taaaacttag tccgatatgc ggcgcgttct gtgcgc
//
LOCUS       DAZKLD010000070        14561 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.070, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000070 DAZKLD010000000
VERSION     DAZKLD010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14561)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14561)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14561
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.070"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            22..834
                     /locus_tag="ACG8KR_001696"
     CDS             22..834
                     /locus_tag="ACG8KR_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HFQ0201063.1"
                     /translation="MLRFFPQKNKYIQEKSEFQFNTDGPIPQHIAIIMDGNGRWAQNR
                     RLPRVAGHKEGMETVKKVTKKASRLGVKVLTLYAFSTENWKRPKDEVSFLMQLPVDFF
                     DTFVPELIKENVKVHVMGYENVLPEHTQDAVRRAIEQTKNNTGMVLNFALNYGSRAEI
                     VTAVQEIAEEVAKGEIHAEEIDDELIAKHLMTGFLPKELQDPELMIRTSGEERISNFL
                     LWQIAYSELYFTKALWPDFDGAHLEEAIASYQNRDRRFGGVKKTEDEKGDQS"
     gene            831..1631
                     /locus_tag="ACG8KR_001697"
     CDS             831..1631
                     /locus_tag="ACG8KR_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HFQ0201064.1"
                     /translation="MKQRVITAAVALVLFIPIIWIGGIAVELTAAILAVVGVYELFRM
                     KGLTLLSFEGVLSAVGAVLLVLPKERWFFFLPENVSTFMLFYLIVMILLGTSVISKNT
                     YTIDEAGFPVLVSLYVGIGFQNFVNARTESLAVLIFGLFIVWATDIGAYMVGRRYGQH
                     KLSPDISPNKTIEGALGGIGSAVVVAFLYLLLYPAKELFNHGMFVMLLMTIIFSAVGQ
                     FGDLVESAIKRHYDVKDSGNILPGHGGILDRFDSLLFVFPIMHLFGII"
     gene            1792..3060
                     /gene="rseP"
                     /locus_tag="ACG8KR_001698"
     CDS             1792..3060
                     /gene="rseP"
                     /locus_tag="ACG8KR_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HFQ0201065.1"
                     /translation="MKTILTFIIVFGILVIVHEFGHFFFAKRSGILVREFAIGMGPKI
                     YGHQAKDGTTYTLRLLPIGGYVRMAGNGDDETEMAPGMPLSLLLNSDGIVEKINLSKK
                     IQLTNAIPMELSRYDLEDELTITGYVNGDETEVVTYPVDHDATIIENDGTEIRIAPKD
                     VQFQSAKLWQRMLTNFAGPMNNFILAIVLFIILAFMQGGVQVTNTNRVGEIMPNGAAA
                     EAGLKENDEVVSVDGKEIHSWNDLTTVITKNPGKTLDFKIEREGQVQSVDVTPKSVES
                     NGEKVGQLGIKAPMNTGFMDKIIGGTRQAFSGSLEIFKALGSLFTGFSLDKLGGPVMM
                     YQLSSEAANQGVTTVISLMALLSMNLGIVNLLPIPALDGGKLVLNIFEGIRGKPLSQE
                     KEGILTLAGFGFLMLLMVLVTWNDIQRFFF"
     gene            3128..4837
                     /locus_tag="ACG8KR_001699"
     CDS             3128..4837
                     /locus_tag="ACG8KR_001699"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HFQ0201066.1"
                     /translation="MRQSKMLIPTLREVPNDAEVLSHQILLRAGYIRQVSAGIYSYLP
                     LANRVLEKLKRIMREEFEKIGAVEMLMPAILPAELWEESGRYETYGPNLYRFKDRNDR
                     KMILGPTHEETFTDLMRNEINSYKKLPLNLYQIQPKYRDEKRPRFGLLRGREFIMKDA
                     YSFHATEESLDETYKDYEKAYTEVFKRCGLEFRSIIGDGGAMGGKDSKEFMAISEIGE
                     DTICYSTESDYAANLEMATSYYVSKKSHETQLELEKVATPNVKSIDEVADFFSVEPQK
                     IIKSVLFIADEQPVLVLVRGDHEVNDVKLKNFLSADFLEEATEEEAQKYLGAEFGSVG
                     PVGVSDDVKVYADRYVQDLANAITGANETGFHYKNVNPKRDFTVLSYEDLRFVQEGDP
                     SPDNNGVLAFTRGIEIGHIFKIGTRYSESMGATVLDENGREKFVIMGCYGIGVSRLLS
                     AIVEQHSDEQGINWPKGIAPFDLHIVQMNLKDEYQTSLTNELEEAMTKAGYEVLVDDR
                     NERAGVKFADSDLIGCPIRITVGKKAVDGIVEVKIKKTGEMVEVRKEELANTLPILLN
                     QGE"
     gene            5049..9401
                     /locus_tag="ACG8KR_001700"
     CDS             5049..9401
                     /locus_tag="ACG8KR_001700"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HFQ0201067.1"
                     /translation="MSEKRDLFEKLLEQIQLEEAEKNHPLLTAGEMDSVVVHRKSRLW
                     EFTLHFSKILPIMLYRSLTQHLTIAFKDIAQVKLNILADDQTFDEQLLQDYWAQALEN
                     QQCDTPLAQQVLKTQVPVIKDRKVILPIDSTGAISYLKQQYLPLVEELFVSYGFPKFR
                     IEPEVDEQQAERVLKLFEERKQEQAEAFMKQAAESLVVHEQKKKERKEQSPALEGPIQ
                     LGRNIPADEPTTPMISIVEEERRVTLEGYVFDKEVRELRSKRKILTLKITDYTSSFIV
                     KKFSNGEKDEQVFDAISVGSWLKVRGSIQEDTFVRDLVMNAQDIIEVKHTPRKDYAPE
                     GEKRVELHVHSNMSTMDATNSISDLVAQAGKWGHRAIAITDHGGAQAFPEAHSAGKKA
                     GVKILYGVEANVVDDGVPIAYNDAHEALSEATYVVFDVETTGLSAVYDTIIELAAVKM
                     YKGNVIESFDEFIDPGHPLSRTTVDLTGITDEMVRGSKSEEEVLRMFLEFSKDTILVA
                     HNAAFDMGFLNTSYARYGIPEAANPVIDTLELARYLYPQFKRFGLGVLSKKFGVSLEQ
                     HHRAIYDAEATGHLAWIFVKEAMDNHNMLYHDQLNEHIGEGDSYKRARPFHVTILAKN
                     QAGLKDLFKLISMSNVEYFERVPRIPRSQLKKMRENLLIGSACDKGEIFEAMMQKGVE
                     EARNRAKFYDYIEVMPKAVYAPLIEQELVKNEHDLEEIIQNLVEIGKSLDKIVVATGN
                     VHYLNEEDAIYRKILINSMGGANPLNRHSLPDVHFRTTDEMLTAFHFLGEETAKEIVV
                     ENTNKIADICEEVIPVKDELYTPKIPGSEDEISELSYTKAKQMYGDPLPEIIQKRLEK
                     ELNSINGNGFSVIYLIAQKLVHKSNEDGYLVGSRGSVGSSFVATMTGITEVNPLAPHY
                     YCPECQYSEFFEDGTYGSGFDMPEKQCPKCGARLNKDGHDIPFETFLGFHGDKVPDID
                     LNFSGDYQAEAHNYTKVLFGEDYVYRAGTIGTVADKTAYGYVKGYERDNNLQFRSAEV
                     DRLAKGATGVKRTTGQHPGGIIVIPDYMDVYDFTPIQYPADDQNSEWKTTHFDFHSIH
                     DNVLKLDILGHDDPTVIRMLQDLSGIDPQTIPTDDPEVMRIFAGPEVLGVSQEQIYSK
                     TGTLGIPEFGTRFVRGMLEETHPTTFAELLQISGLSHGTDVWLGNAEELIRRGDATLA
                     EVIGCRDDIMVYLIHAGLDSGMAFKIMETVRKGQWNKIPDELRETYLSAMKENNVPDW
                     YIDSCSKIKYMFPKAHAAAYVLMALRVAYFKVYFPILYYCAYFSVRADDFDLVSMCKG
                     KDAVKQAMKEITDKGLDASVKEKNQLTVLELANEMLERGFKFGMIDLYKSDAVNFVIE
                     GDTLIAPFRAVPSLGTNVAKQIVEARKDGPFLSKEDLATRGKVSKTLIEYMNDNGVLK
                     DLPDENQLSLFDML"
     gene            9545..10018
                     /gene="rimP"
                     /locus_tag="ACG8KR_001701"
     CDS             9545..10018
                     /gene="rimP"
                     /locus_tag="ACG8KR_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HFQ0201068.1"
                     /translation="MSSVVETVTEMVTPILDEQNFELVEVEFVKEGKSWFLRVFIDKE
                     GGIDIEECAFVSEKLSEKLDAADPDPIPQAYFLEVSSPGAERPLKKESDYEKALGDYV
                     HVSLYQPVDGEKQYEGFLQSFDAEQLTLKIRIKTREKELTFDRKNIAKARLAIQF"
     gene            10042..11217
                     /gene="nusA"
                     /locus_tag="ACG8KR_001702"
     CDS             10042..11217
                     /gene="nusA"
                     /locus_tag="ACG8KR_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HFQ0201069.1"
                     /translation="MSKEMLNALDALEAEKGISKEIVIDALEAALVSAYKRHYGQAQN
                     VEVEFDQKKGKIHVYAVKEVTEEVMDSQLEVSLKDALLINPAYEIGDTIRFEVTPKDF
                     GRIAAQTAKQVILQRVREAERTIIYNEFSAYEKDIMQGIVERQDKRYIYVNLGKIEAV
                     LSKQDQMPNEFYQPHDRIKVYVSRVENTSKGPQVFVSRSHPDLLRRLFEQEVPEVYDG
                     IVEIVSIAREAGDRSKVAVRSNDPNIDAVGTCVGPKGQRVQAIVNELKGENMDIVEWN
                     EDPAVFIANALNPAQVMDVIFDETNPKACTVVVPDYQLSLAIGKRGQNARLAAKLTNH
                     KIDIKSESDMTEFYENQEQQKETEELHDEAIVQSDLTDDEYETIAFNNETVEEKPEA"
     gene            11239..11532
                     /locus_tag="ACG8KR_001703"
     CDS             11239..11532
                     /locus_tag="ACG8KR_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HFQ0201070.1"
                     /translation="MKQRKIPLRKSVVSGEMKPKKEMVRITRSKEGEVSIDPTGKMPG
                     RGAYVSLEPEEVQQAWDKKILDRVLETTLTDEFYQELLDYVTHQKARKELFGK"
     gene            11529..11840
                     /locus_tag="ACG8KR_001704"
     CDS             11529..11840
                     /locus_tag="ACG8KR_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HFQ0201071.1"
                     /translation="MNTVNRQKAMNLIGLAMRAGKLITGEELTIAEIRKQKAKLVFVA
                     TDASENTRKKIKDKSSYYEVPCFELFSEAEITQMIGKPRKVFGIMDAGFAKKTKELIE
                     G"
     gene            11853..14159
                     /gene="infB"
                     /locus_tag="ACG8KR_001705"
     CDS             11853..14159
                     /gene="infB"
                     /locus_tag="ACG8KR_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HFQ0201072.1"
                     /translation="MGNKRIYELAKELKKSSKDVVEKAQQLGIDVKNHMGAITSSDEK
                     KLQQAFKNPSTKQQAAQQASRKPANQQPNDQRKKETTNNQQKNKNNRSYQDRGQGSGQ
                     VNQGKKPTTNQNNTGNTSSQNRQGTSQGGNQNRTNNNNKPNNNNRGKFNNNNRNRYNK
                     KGKKGKQQQSTKPAVPPRKFRELPDVLEYTEGMNVADIAKKIHREPAEIIKKLFMLGV
                     MVNQNQALDKDTIELLATDYGMEPQEKVQVDIADIDKFFEAEEVDPDKLVSRPPVVTI
                     MGHVDHGKTTLLDTLRHSRVTSGEAGGITQHIGAYQIDIDGKPITFLDTPGHAAFTSM
                     RARGASITDITILVVAADDGVMPQTVEAINHAKAAGVPIIVAVNKIDKPGANPQHVMQ
                     ELSEYELIPEAWGGETIFVEISAKFGQNIEELLEMILLVAEVEDLKADPTQRAIGTVI
                     EARLDKGKGPVSTLLVQEGTLHVGDPIVVGNTYGRVRVMTNDLGRRDKEAGPATPVEI
                     TGLNDVPQAGDRFVVFEDEKTARAAGEERAKRALLENRAATSRVTLDNLFESLKEGEL
                     KEVNVIIKADVQGSAEALAASLKKIDVEGVRVKIVHSAVGAINESDVTLAAASNAIII
                     GFNVRPTPQAKVQADTEEVDIRLHRIIYKAIEEIETAMKGMLDPEFEEKITGQMIVRE
                     TFKVSKVGTIAGAYVTEGYIRRDSGVRVIRDGIVIYEGQLASLKRFKDDVKEVKMGYE
                     CGAMIEKFNDIKVDDVIEGFIMEEVKND"
     gene            14186..14533
                     /gene="rbfA"
                     /locus_tag="ACG8KR_001706"
     CDS             14186..14533
                     /gene="rbfA"
                     /locus_tag="ACG8KR_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HFQ0201073.1"
                     /translation="MANYRDRRVGQEILKEVNDILRKKVRDPRVENVTITDVKVTGDL
                     QQATIYYSILSDLASDKKKAQDGLNKASGLIRRELGHNLSIYKTPELFFELDESVVYG
                     NHIDELLRDLNKD"
BASE COUNT         4969 a         2574 c         3245 g         3773 t
ORIGIN      
        1 ttaagtatag gaggaaaaag aatgttacgt ttttttccgc aaaaaaacaa atatattcaa
       61 gaaaaaagcg aatttcaatt taataccgat ggaccaattc ctcagcacat tgccatcatc
      121 atggacggca atggtcgttg ggcgcaaaac cgtcgtttgc cacgagttgc tggccataaa
      181 gaaggcatgg aaacagtaaa gaaagtcaca aaaaaagcat caaggcttgg agtgaaagtg
      241 ttgacgcttt atgccttttc gactgaaaac tggaagcgac caaaagatga agtaagtttt
      301 ctcatgcaac tacctgtcga cttctttgat acgttcgtac cagaattgat taaagaaaat
      361 gtcaaagttc atgtcatggg ttatgaaaac gtattgccag aacatacgca agatgctgta
      421 agacgtgcaa ttgagcaaac aaaaaacaat actggaatgg tattgaattt tgctttgaat
      481 tatggaagta gagcagagat cgtgactgct gtccaagaaa ttgccgaaga agtagcaaaa
      541 ggagaaatac acgcagaaga aatcgatgat gaattgattg ctaagcattt aatgacaggt
      601 ttcttgccta aagagttaca agatcctgag ctgatgatcc gtacaagtgg agaagagcga
      661 attagtaatt ttctgttatg gcagatcgca tatagtgagc tgtattttac aaaagcgctt
      721 tggcctgatt ttgatggtgc gcatcttgaa gaagcaatcg cctcttatca aaatcgagac
      781 cgtcgattcg gcggtgtaaa aaaaacagaa gatgagaaag gggatcagtc atgaaacaac
      841 gtgtgatcac agcagctgtg gcgctagtct tgtttattcc gattatatgg atcggaggca
      901 tagcggttga gcttacagca gcaatcttag cggtagtagg agtttacgag ctgttccgga
      961 tgaaaggatt gacactctta agttttgaag gagttttatc cgccgttggt gcagtgttac
     1021 ttgttttgcc aaaagaaaga tggtttttct ttcttccaga gaatgtaagt acatttatgt
     1081 tattttatct tatagttatg attcttttag gaacatcagt tatttctaaa aatacgtata
     1141 cgattgatga agcaggtttt cctgtgcttg tcagcttgta tgtgggaata ggtttccaga
     1201 attttgtcaa tgcacgaaca gaaagcttgg ctgttttgat atttggtcta tttattgttt
     1261 gggcaactga tatcggagca tatatggttg gtcgacgtta tggtcagcat aagttaagtc
     1321 ctgatatttc gcccaacaaa acgattgaag gtgcgctcgg tggtatcggt agtgctgttg
     1381 tagtcgcatt tctttattta ttgctatacc cagcaaaaga actattcaat catggtatgt
     1441 ttgtcatgct tctgatgacg attatttttt cagcagtcgg acaatttggt gatttggtcg
     1501 aatctgcaat caaacgtcat tatgatgtga aagactcggg aaatatctta ccaggccatg
     1561 gtggaatctt agaccgtttc gacagtttgc tgtttgtatt tcctatcatg catttatttg
     1621 gcattattta gtaagtatac ctgcgtttct ttgtgaattt cataaaagac gcaggttttt
     1681 atttttcatt atattgatac tattggtgtc tttggtatac tatggtaaaa ttatagtgtt
     1741 ttatcgttgt tttataaaaa acacaaaaaa caagtaaagg agaaaaaagc tatgaaaacg
     1801 attctgacat ttatcatcgt ttttggtata ttagtgattg ttcatgagtt tggtcatttc
     1861 ttctttgcga aacgatcagg aatcctcgtc cgcgaatttg ctatcggtat gggtccgaaa
     1921 atttatgggc atcaagcaaa agacgggaca acatatacgc ttcgcctatt acctattggc
     1981 ggttatgttc gaatggcagg caatggggat gatgaaacag aaatggcacc aggaatgcct
     2041 ctttctcttt tactaaattc tgacggcatc gtagagaaga taaatttaag taagaaaatc
     2101 caattgacaa atgctatccc gatggaattg agccgttatg atttagaaga tgagctgact
     2161 atcactggtt atgtgaatgg agacgaaaca gaagtagtaa cctaccctgt tgatcacgat
     2221 gcgacgatta ttgaaaacga cggaacagaa atcagaattg cgccaaaaga cgtacagttc
     2281 caatcggcta aattgtggca gcgtatgctg accaactttg ctggacccat gaataacttt
     2341 attttggcaa tcgtgttatt catcattctg gcatttatgc aaggtggtgt tcaagtgacg
     2401 aacactaatc gcgtaggaga gatcatgcct aatggagctg cggcagaagc aggcttaaaa
     2461 gaaaatgatg aggtagtcag tgtggacgga aaagaaatcc attcatggaa tgatctgaca
     2521 acagtcatta cgaaaaatcc aggtaaaaca ttagacttta agatcgaacg agaaggtcag
     2581 gtgcaatctg tcgacgtgac accgaaatct gtagagtcaa atggcgaaaa agttggccaa
     2641 cttggtataa aagccccaat gaatacaggt tttatggata agatcattgg agggacgcgt
     2701 caagctttca gcggttcatt ggaaattttc aaagccttag gatcactatt tacaggtttc
     2761 agtttagata aattaggcgg acctgtcatg atgtaccaat tgtcttcaga agcagcaaat
     2821 caaggtgtaa caactgttat tagtttgatg gcgcttcttt caatgaatct cggaatcgtc
     2881 aatctgcttc cgattcctgc cttagatggc gggaaattag tcttgaatat ttttgaaggg
     2941 attcgcggga aaccattaag tcaagaaaaa gaaggaatat tgacattagc tggctttgga
     3001 tttttaatgt tgttgatggt gctagttaca tggaacgata ttcaacgatt ctttttctag
     3061 aaaaagttga caaatagggt ataataacaa acgataacta ttttgaggaa ataaaggagt
     3121 atatagaatg agacaatcaa aaatgttgat tccaacatta cgcgaagttc caaatgatgc
     3181 agaagtatta agccatcaaa tcttgctaag agctggatat atccgccaag tatcagcggg
     3241 tatttattca tatctgccat tagcaaatcg tgtattggaa aaactgaaac gcattatgcg
     3301 ggaagaattt gaaaaaatcg gagcggtaga aatgctgatg cctgccatct tgccagcaga
     3361 actttgggaa gaatccgggc gttacgaaac atatggacca aacttgtatc gtttcaaaga
     3421 ccgcaatgat cgaaaaatga tccttggacc aactcacgaa gaaactttta ctgatttgat
     3481 gcgtaatgaa atcaattcat ataagaaatt acctttaaat ctttatcaaa tccaacctaa
     3541 atatcgtgac gaaaaacgac ctcgcttcgg tttgcttcgc ggaagagagt ttatcatgaa
     3601 agatgcatat tctttccatg caacggaaga aagtttggac gagacatata aagattacga
     3661 aaaagcctat accgaagtat tcaagcgttg tggcttagaa tttcgctcga tcatcggaga
     3721 cggcggcgca atgggcggga aagactcaaa ggaattcatg gcgatctcag aaatcgggga
     3781 ggatacgatc tgttattcga ctgaaagtga ttatgctgca aacttggaga tggctacaag
     3841 ctactatgta tcaaaaaaat cgcatgaaac acagcttgaa ttggagaaag tagctacacc
     3901 taacgtaaaa tcaattgacg aagtagcaga tttcttttct gttgaacctc aaaaaatcat
     3961 caagtctgta ttgttcattg cggatgaaca acctgtcctt gttttggtac gtggagacca
     4021 tgaagtcaat gacgtaaaac tgaaaaactt tttatctgct gactttttag aagaagcaac
     4081 agaagaagaa gcacaaaaat atctaggtgc tgagtttggt tctgtcggac cagtcggtgt
     4141 ttcagatgac gtaaaagtat atgcagaccg ttatgtccaa gatttagcga atgccattac
     4201 tggagcgaac gaaacaggat tccactataa aaatgtgaat ccaaaacgcg atttcactgt
     4261 gctttcttat gaagatcttc gttttgtaca agaaggagat ccttcaccag acaacaacgg
     4321 tgttcttgcc tttactcgag gaatcgaaat cggacatata ttcaaaatcg gcactcgtta
     4381 cagtgaatct atgggagcaa ctgtactaga cgaaaatgga cgagaaaaat ttgtgattat
     4441 gggctgttat ggtatcggtg taagtcgttt attatcagca atcgtagaac aacattcaga
     4501 tgaacaagga atcaattggc caaaaggaat tgcgccattt gatctgcata tcgtccaaat
     4561 gaatctaaaa gacgaatacc aaacaagcct gacgaatgaa ctagaagaag cgatgacaaa
     4621 agctggctat gaagtactgg ttgatgacag aaatgaacgc gcaggagtga aattcgcgga
     4681 ttctgatttg attggctgtc caatcagaat cactgtcggt aaaaaagcag tggatggaat
     4741 cgtagaagtg aaaatcaaga aaactggcga aatggttgaa gtacgtaaag aagaattggc
     4801 gaatacttta ccaatcctct tgaaccaagg cgaataataa gcgttaaaac acagaaaata
     4861 aaaatagctg aatcagctgt ttttgacaat ctgatgattc gcttttaaaa ttggaactgc
     4921 gatttgaatc gcagttccaa ttttttaatg taaaactcag aaaaaacgag tatactaaaa
     4981 ggtactgaaa aagaagagga taagatgcga tcaattgcat tgtttctcat cagaaagggc
     5041 gtatatcttt gtcagaaaaa cgagatttat ttgaaaaact gttggaacaa atccaactag
     5101 aggaagcaga aaaaaatcat ccactgctta ctgctggtga aatggatagt gtagtggtcc
     5161 atcgtaaatc gcgtttatgg gaatttacgc tacatttttc caaaattttg cctattatgc
     5221 tatatcggtc attgactcag catctgacta tcgcttttaa agatattgca caagtcaaac
     5281 tgaatatctt agcagatgac cagacattcg atgaacagct cttgcaagat tactgggccc
     5341 aagctttgga aaatcagcaa tgcgatacac cactagcaca gcaagtattg aaaacgcagg
     5401 tacctgtgat aaaagatcgt aaagtcatct tgccaatcga cagtacagga gctatttctt
     5461 atttgaagca acaatacttg ccattagtag aagagttgtt cgtcagttac ggattcccaa
     5521 aatttcgtat cgaaccagaa gtggatgagc agcaagcgga acgtgtattg aaactttttg
     5581 aagaacgcaa acaagaacag gcagaagcct ttatgaagca agcagctgaa agtctagttg
     5641 tccacgaaca aaagaaaaag gaaagaaaag agcaatcgcc agctcttgaa ggacccatcc
     5701 agctaggacg aaatattcca gccgatgaac caacgacacc aatgatcagt atcgttgaag
     5761 aagaacgaag agtcacactt gaggggtacg tattcgacaa agaagtcaga gaactacgtt
     5821 caaaacgcaa gatcttgact ttgaaaatca cagattatac ttcatcattt atcgtcaaaa
     5881 aattctcgaa cggcgaaaaa gatgaacaag tatttgatgc gatcagtgtc ggtagctggt
     5941 taaaggtacg aggaagtatt caagaagata cgtttgtccg tgatttggtc atgaatgcgc
     6001 aagatatcat agaagtcaaa catacacctc gcaaagatta tgctcctgaa ggggaaaaac
     6061 gagtagagct tcatgttcat agcaatatga gtacgatgga tgcaacaaac agcatctctg
     6121 atcttgtggc acaagcagga aaatggggac accgagctat cgccattaca gaccatggtg
     6181 gtgcgcaagc ctttccggaa gcacatagtg ctggaaaaaa agcaggtgtg aaaatcttat
     6241 acggcgtgga agcgaatgtc gtagatgatg gtgtcccaat tgcttataat gatgcacatg
     6301 aagcattgag tgaagccaca tacgtcgtat ttgacgtgga aacgacagga ttgtctgcag
     6361 tatatgacac gatcatcgag ctagccgcag tcaaaatgta taaaggaaat gtgatcgaaa
     6421 gctttgatga atttatcgat ccaggacatc cattgtcacg aacaacggta gatctgactg
     6481 gtatcacgga tgaaatggtt cgagggtcaa agtcggaaga agaagtattg cggatgttcc
     6541 ttgaattttc aaaagataca attttggtag cccacaacgc ggcttttgat atggggttct
     6601 tgaatactag ctacgcaagg tatggtattc ctgaagcggc caatccagtc attgatacgt
     6661 tggaattagc tagatatctg tatcctcaat tcaaacgttt cggattaggt gtgttatcta
     6721 aaaagtttgg tgttagcttg gaacagcatc accgagcaat ctatgatgct gaagcaactg
     6781 gtcacttagc atggatcttt gtgaaagagg caatggacaa tcataacatg ctttatcatg
     6841 atcagctaaa tgagcatatc ggagaaggag attcttacaa aagggctcga cctttccatg
     6901 taacgatttt agcaaaaaat caggcaggac tgaaagattt gttcaaactg atttcaatgt
     6961 cgaatgtcga atacttcgaa cgcgtcccac gaataccacg ctcccaattg aaaaagatgc
     7021 gtgaaaatct attgatcggc tcagcttgtg ataagggaga aatattcgaa gccatgatgc
     7081 aaaaaggggt agaagaggca agaaaccgag caaaattcta tgattatatt gaagttatgc
     7141 caaaagcggt ctatgcgcct ttgatcgagc aagaattggt aaaaaatgaa catgatttgg
     7201 aagaaattat ccaaaatttg gtagaaatcg gaaaaagttt ggataaaatc gttgttgcaa
     7261 caggtaatgt acattatctg aacgaagaag atgccattta ccgaaaaatt ttgatcaatt
     7321 ctatgggcgg agcgaatccg ttgaatcgtc atagtttgcc ggatgtccat ttccgaacaa
     7381 ccgatgagat gctaactgca tttcactttt taggggaaga aacagcgaaa gagatcgtag
     7441 tagaaaatac gaataaaatc gcagatatct gcgaagaagt gattccagtc aaagatgaac
     7501 tttatacacc gaagattcca ggttcagaag atgaaatatc agaattgagt tacacaaaag
     7561 caaaacaaat gtacggagat ccgttacctg agatcattca aaaacgatta gaaaaagagt
     7621 tgaattctat taatggtaat ggcttctcgg ttatttatct gatcgcccaa aaactagtgc
     7681 ataaaagtaa tgaagatggc tatctagtag gttcacgtgg ctctgttggc tctagctttg
     7741 ttgcgacgat gacaggaatc actgaagtta atccattagc ccctcattat tattgtccag
     7801 agtgccaata ttccgagttt tttgaagatg gtacgtatgg ttctggattt gacatgccag
     7861 aaaaacaatg tccaaaatgt ggtgcacgct taaacaaaga cggtcatgat attccatttg
     7921 agacattttt aggattccat ggagacaaag tgcccgatat tgatttgaac ttctcaggtg
     7981 attatcaagc agaagcccat aactatacga aagtattgtt tggcgaagac tatgtttacc
     8041 gtgccggaac gattggtaca gtcgccgaca aaacagcata tggctatgta aaaggatacg
     8101 aaagggacaa taatctgcag tttcgaagtg cagaagtcga cagattagca aaaggcgcta
     8161 ctggagtcaa acggacaacc ggccagcatc caggggggat catcgttatt cccgattata
     8221 tggatgtcta tgattttacg ccgatccaat atccagcaga cgatcaaaat tcagaatgga
     8281 aaacgactca ttttgatttc cactcgattc acgataatgt attgaaatta gatattctag
     8341 ggcatgatga cccaactgtt attcggatgc ttcaagattt atcagggatc gacccacaaa
     8401 cgatccctac ggatgacccg gaagtgatgc ggatatttgc cggtcctgaa gtacttggag
     8461 taagtcagga acaaatttat tccaaaacag ggacattagg tattccagaa ttcggcactc
     8521 gttttgttcg agggatgtta gaagaaacgc atcctacaac ttttgcagaa ctactgcaaa
     8581 tttccggact atctcatggt acagacgtat ggttaggaaa tgcggaagaa ttgattcgac
     8641 gaggagatgc gacactggcg gaagtaatcg gctgtcgtga tgatattatg gtttatttga
     8701 tccatgctgg attagatagc gggatggcat ttaaaatcat ggaaactgtt cgtaaaggac
     8761 aatggaacaa gatccctgat gaattgcgag aaacctatct atcggcgatg aaagaaaata
     8821 atgtgcccga ctggtatatc gattcttgtt caaaaatcaa atatatgttt ccgaaagccc
     8881 atgctgcagc ttacgtattg atggctcttc gtgtcgctta tttcaaggtt tatttcccta
     8941 ttttgtatta ctgtgcatac ttttctgtac gtgcagacga ctttgatcta gtctccatgt
     9001 gcaaaggaaa agatgctgtg aaacaagcaa tgaaagaaat cacggacaag ggattagatg
     9061 cttccgtaaa agaaaagaat caactgacag tgctggagtt agccaatgaa atgctggaac
     9121 gaggattcaa atttggaatg attgatttgt ataaatcgga tgccgttaac tttgttattg
     9181 aaggtgatac gttgatcgca ccatttagag ctgtcccaag tttgggaacc aacgtggcga
     9241 aacaaatcgt tgaagcaaga aaagacggtc catttttatc caaagaagat cttgccaccc
     9301 gcggtaaagt ttcaaaaaca ttgatcgaat atatgaacga caatggcgta ttgaaagatc
     9361 taccagatga aaatcaatta tcgttattcg atatgctata agcatctgtt ttcctgtctg
     9421 cttgtaattt taagcagagt atggtatagt tcttataggt aatgatacta actgtgagtg
     9481 agcggatttt tcgctcactc tttttaatgg aattacgcac ttaattttta aggaggcgaa
     9541 acttttgagt agcgtcgttg aaacagtcac agaaatggtg acaccgattt tagatgaaca
     9601 aaattttgaa cttgtcgaag tcgaatttgt aaaagaagga aaaagctggt tcttacgagt
     9661 atttattgac aaagaaggcg gtatcgatat cgaagaatgc gcctttgtca gtgagaaact
     9721 gagtgaaaaa cttgatgcag cagatccaga tccgattcct caagcttatt tcctggaggt
     9781 atcctccccg ggagcagagc ggccattgaa aaaagaaagc gactatgaaa aggcacttgg
     9841 tgattatgtc catgtctcct tataccaacc agtagatggc gaaaaacaat atgaaggatt
     9901 tcttcaatca tttgatgctg aacagcttac attgaaaata cgcataaaaa cacgagaaaa
     9961 agaattgact tttgaccgaa aaaatattgc aaaagctcgc ttagctatcc aattttaatc
    10021 acggagagga atcagaaaaa aatgagtaaa gaaatgttaa atgcgttaga cgctttagaa
    10081 gctgaaaaag ggatctcaaa agaaatcgtg atcgatgctt tagaagcagc attggtttct
    10141 gcatacaaac gtcactatgg ccaagcacaa aacgtcgaag tggaatttga tcagaaaaaa
    10201 ggaaagatcc atgtctatgc ggtaaaagaa gtaacggaag aagtgatgga ttcacagttg
    10261 gaagtctcat tgaaagatgc acttttaatc aatccagcat atgagatcgg tgatacgatc
    10321 cgttttgaag taacaccaaa agattttgga cggatcgctg cgcaaacggc taaacaagtt
    10381 attttacagc gtgtacgtga agcagaaaga acgattattt ataacgaatt cagtgcttat
    10441 gaaaaagaca tcatgcaagg aatcgtcgaa cgccaggata aacgttatat ttatgtcaat
    10501 cttggtaaaa ttgaggcagt tctttctaaa caagatcaaa tgccaaatga attttatcaa
    10561 ccacatgatc gaatcaaagt ttatgtttcc cgagtagaaa atacgtctaa aggtccgcaa
    10621 gtatttgtta gccgcagcca tccagaccta ttgcgtcgct tatttgaaca agaagtacca
    10681 gaagtctatg atggaatcgt tgaaattgtc agcatcgccc gtgaagctgg cgatcgttca
    10741 aaagtggcag ttcgctcaaa tgatccaaat atcgatgcag tcggtacatg tgtcgggcca
    10801 aagggacaac gagttcaagc aatcgtcaat gaattaaaag gcgaaaacat ggatatcgtc
    10861 gaatggaatg aagatccagc tgtattcatc gcaaatgcat tgaatcctgc acaagtcatg
    10921 gatgtgatct tcgatgagac aaatccaaag gcctgcacag ttgtcgttcc agactaccaa
    10981 ttatcgctag caatcggtaa acgcggacaa aatgcacgtt tagctgctaa attgactaac
    11041 cataaaatcg atatcaaatc agaatctgac atgacggaat tttatgaaaa tcaagaacaa
    11101 caaaaagaaa cagaagaact gcatgatgaa gcaatcgttc agtcagattt aacagacgat
    11161 gaatatgaaa caattgcatt caacaatgaa acggtcgaag aaaaaccaga agcttaactt
    11221 tagtaaggag gcaaagagat gaaacaaagg aaaattcctt tacgcaaatc cgtggtttca
    11281 ggtgaaatga aacctaaaaa agagatggtc cgaatcaccc gttcaaaaga aggggaagta
    11341 tcgatcgatc caactggaaa aatgcctgga cgaggagcgt atgtctcact tgagccggaa
    11401 gaagtacaac aagcttggga taaaaaaatt ctcgatcgag tactcgaaac aacattaaca
    11461 gatgagtttt atcaagaact tcttgactat gtcactcacc aaaaagcacg taaagagtta
    11521 tttggaaaat gaatacagtt aatcggcaaa aagccatgaa tctcatcggt ctagcgatgc
    11581 gcgcgggaaa actaatcacg ggtgaagaac tgaccattgc tgaaatccgc aaacaaaaag
    11641 caaaattggt gtttgtggca accgatgcaa gtgagaatac aagaaaaaag ataaaagata
    11701 agagttcata ttatgaagtt ccttgctttg agctattttc tgaagcagaa atcacgcaga
    11761 tgatcggaaa acctcgcaag gtattcggaa taatggatgc cggatttgcg aagaagacca
    11821 aggagctaat tgaaggttag gaaggtgatt gcatgggcaa caaaagaatc tatgaattag
    11881 caaaagaact caaaaaatct agtaaagacg tcgtggaaaa agcacaacaa cttggcatcg
    11941 atgtgaaaaa ccatatgggt gcgattacta gcagtgatga gaagaaattg caacaagcgt
    12001 tcaaaaaccc aagcacgaaa caacaagctg cacaacaagc aagcagaaaa ccagccaatc
    12061 aacagccgaa cgatcaacgt aaaaaagaaa caacgaacaa tcaacaaaaa aataaaaata
    12121 accgcagtta tcaagatcgc ggtcaaggga gcggtcaagt gaatcaagga aaaaaaccaa
    12181 caacaaacca aaataatacg ggaaatacca gcagccaaaa tcgtcaagga acgagccaag
    12241 gcggcaatca aaaccgtact aacaataaca acaaaccaaa caataacaat cgcggcaaat
    12301 ttaataataa taaccgtaat cgttataata aaaaaggtaa aaaaggcaaa cagcaacaat
    12361 caactaaacc agctgtacca ccacgcaaat tccgtgaatt gccagatgta ttagagtata
    12421 ctgaaggtat gaacgtagca gatattgcga aaaaaattca tcgtgagcca gcagaaatca
    12481 ttaaaaaatt atttatgctt ggtgtcatgg tcaatcaaaa ccaagcactg gataaagata
    12541 cgattgaact gttagcgacg gactatggca tggaaccaca agaaaaagtc caagttgaca
    12601 ttgctgatat cgataaattc ttcgaggcag aagaagtcga tccagataaa ttagtctcac
    12661 gtccgccagt agtaacgatt atgggacacg tagaccacgg gaaaacaacc ttattggata
    12721 cattgcgcca ttcacgtgta acaagcggcg aagccggcgg aatcacacag catatcggtg
    12781 cctaccagat cgatattgat ggtaaaccaa tcactttctt ggatacacca ggacatgcgg
    12841 cctttacaag tatgcgtgca cgtggagcaa gtatcacaga tatcacgatc ttagttgttg
    12901 ctgcagatga tggcgtcatg ccacaaacag ttgaagcaat caaccatgcc aaagctgcag
    12961 gcgttccaat catcgtcgct gtaaataaaa ttgataaacc aggtgcaaat cctcagcatg
    13021 ttatgcaaga attaagtgag tatgaattaa ttcctgaagc atggggtgga gaaacgattt
    13081 ttgttgagat ctcagcgaaa ttcggccaaa acatcgaaga attgctggaa atgatcctgt
    13141 tagtcgcaga agtagaagat ttaaaagcag atccaacaca acgcgcaatc ggaacagtga
    13201 tcgaagcacg tcttgataaa ggtaaaggtc ctgtgtcaac cttacttgtc caagaaggaa
    13261 cacttcacgt tggtgaccca atcgttgtcg gtaatactta cggacgtgtc cgtgtaatga
    13321 caaacgatct tggccgtcgc gataaagaag caggacctgc aacgccagta gaaatcactg
    13381 gcttgaatga tgtacctcaa gcaggtgacc gtttcgtagt cttcgaagac gaaaagacag
    13441 cacgtgcagc gggtgaagaa cgcgcaaaac gtgcattatt ggaaaaccgt gctgccacaa
    13501 gccgtgtgac tttggacaac ttatttgaaa gcctgaaaga aggcgaattg aaagaagtta
    13561 acgtaatcat caaagcagat gtacaaggtt cagcagaagc actggctgca tcattgaaga
    13621 agattgatgt ggaaggtgtc cgtgtaaaaa tcgtccattc agctgtcgga gcaatcaatg
    13681 aaagtgatgt aaccttagca gcagccagca acgctattat catcggtttc aacgttcgtc
    13741 ctacaccgca ggcaaaagtg caagctgata ctgaagaagt cgatattcgt cttcaccgta
    13801 tcatctataa agccatcgaa gaaatcgaaa ctgcgatgaa agggatgcta gaccctgaat
    13861 ttgaagaaaa aatcaccggt caaatgatcg ttcgtgaaac cttcaaagtt tctaaagttg
    13921 gtacgattgc aggtgcctat gtaacagaag gatatattcg tagagatagc ggtgtccgtg
    13981 taatccgtga cggaatcgta atctatgaag gccaacttgc aagtctgaaa cgattcaaag
    14041 atgacgtgaa agaagtcaaa atgggttatg aatgtggtgc gatgattgaa aaattcaatg
    14101 acatcaaagt agatgacgta attgaaggat ttatcatgga agaagtcaaa aatgactaaa
    14161 aactgataaa aggaggccaa tcattatggc taactaccgt gatcgccgag tcggtcaaga
    14221 aatcttaaaa gaagtcaatg atattttgcg caaaaaggtc cgtgaccctc gtgtggagaa
    14281 cgtaacgatc acagatgtga aagttacggg ggacttacag caagcaacga tctattacag
    14341 cattttatct gacttagcat cagataagaa aaaagcacaa gatggattga ataaagcatc
    14401 aggattgatt cgtcgagagt tagggcacaa tctaagtatt tataagacac ctgaattgtt
    14461 ctttgaactt gatgaatcag ttgtctatgg gaaccatatc gatgaattgc ttcgcgactt
    14521 gaacaaagac taatcaaaaa atcccgcaaa tcctgtgaaa t
//
LOCUS       DAZKLD010000071        14419 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.071, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000071 DAZKLD010000000
VERSION     DAZKLD010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14419
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.071"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..193)
                     /locus_tag="ACG8KR_001707"
     CDS             complement(<1..193)
                     /locus_tag="ACG8KR_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0201074.1"
                     /translation="MKSLEQEHMAFKQAMFKENIYLNHNYIRVSKACSPVLNMLGGGN
                     GLYHLLFVDVCWLIFLPDEL"
     gene            complement(200..748)
                     /locus_tag="ACG8KR_001708"
     CDS             complement(200..748)
                     /locus_tag="ACG8KR_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HFQ0201075.1"
                     /translation="MKRIIKIFGVLGVFLLGLSTFVSTGTSVYATEKVNTVDLEIKNK
                     LVDELTFYFDTVGFLDEHGVYHIKDFGAIRERISQDNEQGRAARVFYEHIIVPRMEKV
                     DWKRYAGCVAINSLPGGGVAWAIGTQGDFIEALKAFNFEKAADLLLKIAEKQLGKAAA
                     KELAKVNIVAGIAAAAASCALE"
     gene            complement(793..1125)
                     /locus_tag="ACG8KR_001709"
     CDS             complement(793..1125)
                     /locus_tag="ACG8KR_001709"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0201076.1"
                     /translation="MSEEKKEIVESLVALKDSLSKIEYVDRQEIQKLIDDTIIEIQDA
                     RCEGIKISVALSKVIEKMNRSLAFNGLKLDRQTSLIWDHLKDLYDKSKRSERTAVSIL
                     KGLWGMNS"
     gene            complement(1139..1396)
                     /locus_tag="ACG8KR_001710"
     CDS             complement(1139..1396)
                     /locus_tag="ACG8KR_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003653123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0201077.1"
                     /translation="MKKLIGYSVMCIGFVGVGLFVSTESASAAEWYNPIWYNPNIECQ
                     KGYPYGPTNLKNGKCRVNWNGVVDSVITGSMDSALHGFTGH"
     gene            complement(1591..2133)
                     /locus_tag="ACG8KR_001711"
     CDS             complement(1591..2133)
                     /locus_tag="ACG8KR_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF0163 family protein"
                     /protein_id="HFQ0201078.1"
                     /translation="MKKGIILVFSWLCLIGLTGCHWFSSEKQKTQLRTTEETIEAVKE
                     TSGTLQSTESNSQIGSLEDETAQQMQIDEMTKNFLKDFARKWSNFTDVYKRNQAVRDY
                     MTDRCIKDNSIDVDPHVQMKTTGKLYQVTQDIENPDQYLLFGEETMNKVTQFVLLQLK
                     VDQEAQKIDAIKIYYVRSAY"
     gene            complement(2154..3245)
                     /locus_tag="ACG8KR_001712"
     CDS             complement(2154..3245)
                     /locus_tag="ACG8KR_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFQ0201079.1"
                     /translation="MKKGCLGCLLFFILPFFLLPFFFSGELSEHGGEATGLAVAFNGE
                     YTEGLPIFSEIKGRGPITDEHAQAAVGAAVKYQLLPSVILSQLGYESAYGQSQSGRND
                     TNFFGITWFPGCPYPQGSARGIGGSEGGFYMKFPNLKACYDYYGFMVASQSNFNQCVG
                     NKDPGSCLLILGRGGYAAAGITEGSPYYRGAMSIITSYNLTEYDTFAIKHWNSLPTKK
                     TSLIAGKSKGNAKVLDRIIGRSVNNGECYGGTAWYVSQLGGPQLMGSGHMFAMLIGED
                     YNWTTYGWQVIMNPKPSDLQAGDVVNWQAGGALSPGIYGHTGIITDVSYGGQHFSSVE
                     QNAEQGRIMARYQRTYNQTKIRSLVRKVK"
     gene            complement(3253..5463)
                     /locus_tag="ACG8KR_001713"
     CDS             complement(3253..5463)
                     /locus_tag="ACG8KR_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD3337/EF1877 family mobilome membrane protein"
                     /protein_id="HFQ0201080.1"
                     /translation="MAYIKHKEKKWCVLFFLTIGFGLLFVGGLVYAAEKQVDQSIYEI
                     GKFHIDTFSSYLSDSEFSLGKMLFGANGTDFLKMMTNMFFSLSKLIWQGLDFVIEKLY
                     EGAAMNELIHQFFTFSDTIYSKIYDPIGAGIVVLYVLYLFTLRIFRGKHYAKMVFIRF
                     LFVVIFSMVWFGYGSNVPSKGEAFTKGLNDLSVEVEGFIFSATNSLDELATKDVNGVL
                     VPTTQKEAIQKVRQLYYKAAVVDPYILLNYGTTNLEELYQAEIEPQEFLAKEASSEEL
                     SDIETKVTTAAGEDRGKKKAYRVYIQPTKAVYKAVVGSMVPLLNLSIGLPLVVIGMIR
                     FIFQMLVLCLLLVIPFSLLASFVPVFDYMLMNTLKSFLGLMFQKSFYSVIILVAFLIY
                     NIVDDLIPMNTVFGFLGNMFVKALLSILLIVKRKDLLKKLGLGAADQTINNTKNIASH
                     SLSQMKQLPGQLSTAGQNLAIKGAGLAGNVYRATNPLASAAIHGFQKFQQKRTPQESV
                     KGRQVVNPFELGGRSNQALPYSGRHVRYHGVEPEMSSTKSKTEWNSGLSNGLIPNSSM
                     NSVRPKQQVQSLAQLSKQQGNNQRKPLLNKGIAFVGQSIPLPSGKVPVPQLTAAGSLS
                     QQIRQVKNQAKNGSYPAMQQLLNQYSVPNSSVATLSGNRSVQQPGQRSSESKPSISAP
                     KSLQPAFSSLDKPTEQSEKIEKDPKTLPILDAKPGTDFTLPKGRTVQKPLSKKG"
     gene            complement(5479..8007)
                     /locus_tag="ACG8KR_001714"
     CDS             complement(5479..8007)
                     /locus_tag="ACG8KR_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFQ0201081.1"
                     /translation="MVLVKEPVKAFKKNCLLNGEDCIWAYYHVFPRTISKNTPKKTQE
                     IKEMMARLLLLLADYEDVELVMYPRTMNLRTKFDTLGKGFSPDSQAIGAYYAKETVTL
                     LEREGQVYRPSFILGIKVKEKATVESFHDVINLIWLDLAGSLARLFGLEVDDLETLFS
                     ESDPYVKEVTQLLYSFSIRPLVKKELLYLNRLQYIRGMAHKVEEQNNWEVLKDNQGTY
                     VRIPKEHKGLLQLEDELGSSYLALLPIGETPENMRNCDLFQFAQDLSFPAELRMKIHY
                     PRLKGQGGIGLKLNWLSERFKTEHKELVQNDEEPSERLLTVRGLVKHLKQRVDNGQPV
                     VDWIACFIVYGQTVEEVRQNSATVIQQLHTIIHIHRAKNDQRELFYKCLLGQPLGRQL
                     HWKHRTTVEAVGEWLFSAVSELGMESGWYIGRQDTLGDGGSRGEKTPLEELIYASNRF
                     VFLNLLAIAEGIKGALFDAPHVAVTGKTGKGKTFLVGLLFLYSSFMNVQSLFVDPKGE
                     KRKWFTKLIQDPYYQKHYPLFVEHLQKMSYVTLDATNPRNYGVLDPIVCLSRVDAKQV
                     AQDMIDELSPLGTNHLLKGAVLKGIQEVLVQKEAGQQVGLMHVVDYLEQMEQKEVREY
                     GEFLRLTVEDSILRLGFSYGENPGLDFNAKTTILEIQDLKLPNDNVRPELYTDADRKS
                     LCLMISLGRFCEMFGKRDSSKKTAIYFTEAWVFNNSNAGRSIIAAMARVGRSQMNQLV
                     LDTQFIGDLGSDKEKGNFGVVFAFDEDSEREKILNHLRLEVNEANLAEMSHMIKGQCW
                     MLDPYGRVGKLNVHSLFEEMTAALQTTEKTSHSQVEEQDTLIMQ"
     gene            complement(8059..8469)
                     /locus_tag="ACG8KR_001715"
     CDS             complement(8059..8469)
                     /locus_tag="ACG8KR_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TcpE family conjugal transfer membrane protein"
                     /protein_id="HFQ0201082.1"
                     /translation="MDWEPYDYSIVYAEPYRVQQITSNWRLPFPVALVNAATFLLVVI
                     FEYLFLRSFIGWVISFFPPAGVVFYIGLPYLCVLALAKLKPDGKKVHFYLWDWFCYMI
                     RMMNGDCVYYNWDKVSNNALEELLFVNKYVYLED"
     gene            complement(8482..8700)
                     /locus_tag="ACG8KR_001716"
     CDS             complement(8482..8700)
                     /locus_tag="ACG8KR_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TcpD family membrane protein"
                     /protein_id="HFQ0201083.1"
                     /translation="MTLKGIVDFILTQGGYFLIMVVVYLGLRNFMKSKVGNALISIAA
                     GAAVYFFLNDPTRVFDAFGKIIEKLFMD"
     gene            complement(8697..9689)
                     /locus_tag="ACG8KR_001717"
     CDS             complement(8697..9689)
                     /locus_tag="ACG8KR_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFQ0201084.1"
                     /translation="MKKRQLRRKEKVYTEKVPKQKKIHSKKAVKWIWCLVLFLCFSGG
                     LAFFQSMKTKVQVSFLKDEIQQLQKKEVQSRSELTYTPEIESFMNRFVALYMNTFDNH
                     EQEQQRQETLKKEFYAQNFIQEPGSVSGVRKLTAASFIGLKEIETIPTASYKVTYDIR
                     SMNPESKKETSVTITQVLNLPFSISNKGCRIIAYPYFTALPKNTEKQRMITYDDTLYE
                     TVDASEAKDVEGFVEDFLDKYAKNNAKDMSYMMKEPEGLNQTAKVDQVEMKVLRYKET
                     KKPFIVKATVVFRDGDTSLLQKEQMTIVITKKTNQYYVEKLTHNWLDRKGEERK"
     gene            complement(9768..10679)
                     /locus_tag="ACG8KR_001718"
     CDS             complement(9768..10679)
                     /locus_tag="ACG8KR_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HFQ0201085.1"
                     /translation="MKKTTLAVVTTLGISLFSFQLGSSLVYADETVETPKSEIVETLP
                     GEGLPDVETELNGGTTEKPAEPEQLQSEKPEKEPVTPEPEFPESTLPTDSIEEEGVHS
                     STTTDSSIVTTPEQSGTTSSSEKNTDSSSTEQPTMSSTSEEQKEEPKQPVEKSEAIPK
                     ENKPIVPAKEVTVSVTPSGEITTNTSQGMSVPIVTSNVEELTHIPTPTTPLKVAGDQT
                     IVGVKDGIPLIQDSEGNLVKDLSIPVKKLPSGNIEIKTADGKTKVLPKTGEEIHVALS
                     VLGGVLTSIAGFVGYKRKRKGIENEGN"
     gene            complement(10698..11042)
                     /locus_tag="ACG8KR_001719"
     CDS             complement(10698..11042)
                     /locus_tag="ACG8KR_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0201086.1"
                     /translation="MWRLLFLVVCGMALFYVYRVYLYPRMERWTSWYRDPLKQRKGLK
                     RYRYELLVEFSDGRQAQYYFFANYPKYNTSELLIQLFKNNPYVGLEENGREFFYRSDN
                     ISAASMNKTRIA"
     gene            complement(11062..11340)
                     /locus_tag="ACG8KR_001720"
     CDS             complement(11062..11340)
                     /locus_tag="ACG8KR_001720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0201087.1"
                     /translation="MSVEHFCDCYIALLRSQQLLTVERYGAEFGYVLQAERHRATRCY
                     EKEDQVIDGYEELCIRNNRYLQQRLYEQLMMDFSNLYRGYECSMIQER"
     gene            complement(11699..12199)
                     /locus_tag="ACG8KR_001721"
     CDS             complement(11699..12199)
                     /locus_tag="ACG8KR_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0201088.1"
                     /translation="MKKIMIYVGAYWSRDPTVLENPEAVHYCLRQLFYLYKERLESLI
                     RQLPYTDRRLDELLLRYPAMYKRRKNRLLPEEYPIEKHELEGRFVAYFYDDVRMRLVE
                     QRMEIENDRYYFINYCKKRKYQVTDDFYDCILQDGEVILSKIAPSFRELVPIDFLKKC
                     HLRILP"
     gene            complement(12200..12745)
                     /locus_tag="ACG8KR_001722"
     CDS             complement(12200..12745)
                     /locus_tag="ACG8KR_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0201089.1"
                     /translation="MNAYTTTEWLRLLDLKAAIEALNEKMVDLSYFRFRVPYIEQAVK
                     AGRYQEKENWQEIARLLEVRKGYEQELEELEFSRRKGRLEFIRFYRFSLPIPAILAVK
                     KGCDKMKIYENCVAALSSEKPLMEEISLVTVTWEMSRQPTEEQTYLSLEEIEIELEEI
                     GRYATCSTYCGSVISIAGVIV"
     gene            complement(12742..13998)
                     /locus_tag="ACG8KR_001723"
     CDS             complement(12742..13998)
                     /locus_tag="ACG8KR_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFQ0201090.1"
                     /translation="MTPIELKSYRKKLSAIHNISGDKKPLSQARFAQLMGCSKDYIRK
                     MEQGKRKIPEDFEQRIKERFRLYMKSGIDLRAMIDYLRLSFFGTDYKTVIQRVLRMDL
                     TEFETRPTGLYLFDCLSVRGNIWVFWHSKRTVTENVLVQFSGQGCREYEEVMREQQTD
                     WRTWLYSIWKNQGKLEAGYTRVQCSRFDIALDELWGLETEEHFDLFELLEKQKAGLLE
                     MDFKRFQNIGGCFLDDGYFRSEGLSLYFGSRKSPLFFNFYEKRFEIANREKISELEAL
                     TKYGIYNRYELRLANEKATQAVEAFILGDSPKRLGEIGVGLINAKLIVWDRLESDARI
                     VDKRWAKLFRSMEELDFTVKPEKFSLDRSERWFLKQVAPTLKLLQEADRILGRDFVQE
                     GIDNAELNDEQQNYLEWMGTSGGKIA"
BASE COUNT         4296 a         3134 c         2091 g         4898 t
ORIGIN      
        1 ctagttcatc tggtaaaaaa attaaccaac aaacatcgac aaacagtaaa tgatataatc
       61 catttccacc tccaagcata ttcaatactg gtgaacatgc cttagaaaca cgaatatagt
      121 tatggtttaa atagatattt tctttaaaca ttgcttgttt aaaagccata tgctcttgtt
      181 ctaaactttt cataaacttt tactctaatg cacagctagc agcagctgct gcaattcctg
      241 ctacaatatt taccttagct aactctttag ctgcagcttt tcctaattgt ttttctgcaa
      301 tttttaataa taaatccgca gctttttcaa aattaaaagc ttttaatgct tcaataaaat
      361 ctccctgcgt acctatagcc caagccactc ctcctccagg aagagaatta atggctacac
      421 atccagcata tcgtttccaa tcaactttct ccattctagg aacaataata tgttcataaa
      481 agactcttgc tgctcttcct tgttcgttat cttgactgat tcgttcacga atagcaccaa
      541 aatcctttat atggtataca ccatgttcgt ctaaaaaacc aactgtatca aaataaaaag
      601 ttaattcatc tactaattta ttctttattt ctaaatcaac tgtattcact ttttctgtgg
      661 catacacact tgttccagta ctaacaaaag tacttaaacc taataagaaa acacctaaaa
      721 caccaaatat tttaattatt cttttcataa tttctcctcc ataaaaaata agttactttt
      781 taaatatctt gtttatgaat tcattcccca aaggcctttt aagattgata cagcagtccg
      841 ttcacttctt ttagacttgt catacaagtc ttttaaatga tcccatatca gactagtttg
      901 tctatctagc ttcaatccat taaacgctaa tgaacggttc attttttcta ttactttaga
      961 taaagctaca gaaattttta taccttcaca acgcgcatct tggatttcaa taatagtatc
     1021 atcaattagt ttttgaattt cttgcctatc aacatattca atcttagaaa gagaatcttt
     1081 taaagctact aatgattcaa cgatttcttt tttctcttca gacattttct tcctccttct
     1141 agtgacccgt aaagccatgt agagcagagt ccattgaacc agtaattaca ctatctacaa
     1201 cgccattcca atttactcta cattttccat ttttcagatt tgtcggtcca tacggatagc
     1261 ctttttgaca ttcaatgttc ggattatacc agataggatt ataccattca gccgcagaag
     1321 cagattcagt cgatacaaac aatcccacac caacaaatcc aatacacatc acactatagc
     1381 caattagttt tttcatctcg tatccatctc ctaaaaaata gaatagttct tatagttatt
     1441 cataagacat attttttccg tttttgtttt atcctttctg tcctataaca acttttttca
     1501 agtaaagaat gacatagttt ttcttaaaaa aacagtctgt ttttttctat ttcttcggtg
     1561 tataataaat atgagtgttc attttttatt ttaatacgca ctccgtacat aataaatctt
     1621 gatcgcatca atcttttgag cttcttgatc tactttcaat tgaagtaata caaattgagt
     1681 aaccttattc attgtttctt ctccaaataa caaatattga tccggattct ctatatcttg
     1741 tgtcacctgg taaagtttcc cagttgtttt catttgtaca tgcgggtcta catcaatact
     1801 attgtcttta atacaacgat ctgtcatata gtccctcaca gcttgattgc gtttataaac
     1861 atctgtgaaa ttactccatt ttctagcaaa atcttttaag aaattttttg tcatttcatc
     1921 tatttgcatt tgttgcgctg tctcgtcttc taatgaacca atttgactgt tactttcagt
     1981 actttgaaga gttccacttg tttcttttac tgcttcgatt gtttcttctg tcgttctcaa
     2041 ttgtgttttt tgtttttcag aagaaaacca atgacaacca gtaagaccta tcaaacataa
     2101 ccaactaaat actaaaatta tccctttttt cacgtttcta gtcactgcct ttcttatttc
     2161 accttacgaa ccagactacg aatttttgtt tgattgtacg tgcgttgata ccgagccata
     2221 atccgtcctt gctcggcatt ctgttccaca ctagagaaat gttgtccgcc atatgaaaca
     2281 tctgttataa ttccggtgtg accatagatt ccaggagaca atgcaccacc tgcttgccaa
     2341 ttcaccacat ctcctgcttg taaatcactt ggttttgggt tcatgattac ttgccaacca
     2401 taggttgtcc agttataatc ttcccctatt aacatcgcaa acatgtgtcc agaacccatt
     2461 aactgtgggc cacctaattg agatacatac catgccgtac caccataaca ttccccatta
     2521 tttacggatc gtccaatgat ccgatccaaa accttcgcat tgcctttact ttttcctgca
     2581 ataagcgaag ttttttttgt tggtaggcta ttccaatgct tgatcgcaaa agtatcatat
     2641 tccgttaagt tataggacgt tatgatactc attgctcctc gataatacgg actcccttcg
     2701 gtaatacctg ctgccgcata gcctccacga cctagaatca ataaacaact tccaggatct
     2761 ttattgccca cacattgatt aaagttgctt tggctcgcca ccataaagcc ataatagtca
     2821 taacaagctt ttaagttcgg aaacttcata tagaaaccac cttcagaacc accaattcca
     2881 cgtgcagaac cttgtgggta gggacaacca ggaaaccagg taatcccaaa aaagttcgta
     2941 tcgtttcttc cagattgtga ctgtccataa gctgattcat agcctaattg agaaagaatc
     3001 acactcggta ataactgata ctttacagct gcaccaactg ctgcttgcgc atgttcatct
     3061 gtgattggac cacgtccttt aatctctgaa aaaatcggta atccttcggt atattcccca
     3121 ttaaaagcga cagctagacc tgtagcttct cctccatgct cagaaagttc tcctgaaaaa
     3181 aagaatggca atagaaaaaa gggaagaata aagaaaagaa gacaacctag acaccctttc
     3241 ttcatttctt cctcatcctt tcttcgataa tggtttttgg actgttcgtc cttttggtaa
     3301 agtaaaatca gttcctggtt ttgcgtccaa aatcggcagt gttttaggat ccttttctat
     3361 tttttccgat tgctctgttg gcttatctaa agaagaaaat gcaggttgta acgattttgg
     3421 tgcggaaatc gacggtttac tttcagacga acgttgcccc ggttgttgca cagaacgatt
     3481 tcctgataga gtggctaccg acgaattagg aacagagtat tggttcaata actgctgcat
     3541 tgctggataa ctcccatttt tcgcctgatt tttcacttgg cgaatctgtt gtgaaagaga
     3601 accagcagct gttaattgtg gcacgggtac ttttccagaa ggcaacggaa tcgattgacc
     3661 aacaaaagcg atacctttat ttaacaatgg ttttctttga ttattccctt gttgcttact
     3721 caattgtgcc aatgattgta cctgttgttt tggtcttact gaattcatgg acgaatttgg
     3781 aatcaaacca tttgaaagac cagaattcca ctctgttttc gatttggtag aagacatttc
     3841 aggctctacc ccatgataac gaacatgacg accactataa ggcaaggctt gatttgaacg
     3901 cccacctagt tcaaagggat taaccacttg cctccctttt accgattctt gtggtgtacg
     3961 tttttgttga aacttttgaa atccatgtat cgctgcagaa gcgagtggat tcgttgctcg
     4021 atacacattc cctgctaatc ctgcaccttt gattgccaaa ttttgtccag cagttgatag
     4081 ttgaccaggc agttgtttca tttgagacaa agaatgactt gctatgtttt tcgtgttgtt
     4141 tatagtttga tctgcagctc ctaaccctag tttctttaat aaatctttcc gttttacaat
     4201 gagtaaaatt gataggagtg ctttgacaaa catatttcct aaaaaaccaa aaacagtatt
     4261 cattggaatt aaatcgtcta ctatgttata gatcaaaaat gccaccaaaa taatcacact
     4321 gtaaaaagat ttttgaaaca tcaagcccaa aaaggacttt aaggtattca tgagcatata
     4381 atcaaatact ggaacaaaag aagcgagtaa tgaaaaagga atcacaagta ataaacacaa
     4441 caccaacatt tgaaagataa aacgaatcat gccaatcact acaagtggta atccaatact
     4501 tagattaagc aatggcacca tagaaccaac gactgcttta taaacagctt tagttggttg
     4561 aatatagaca cgataagcct tctttttccc tctatcttct cctgcagcag tggtcacttt
     4621 tgtctcaata tcagataatt cttcacttga cgcttccttg gctagaaatt cttgtggttc
     4681 aatctctgct tgatacaatt cttctaaatt tgttgtccca taatttaaca atatatacgg
     4741 atcaacaaca gccgctttat aataaagttg gcgcactttt tgaatggctt ccttttgtgt
     4801 cgttggcact aaaacaccat tgacatcttt tgtcgccaat tcatctagac tatttgtagc
     4861 agaaaaaata aagccttcta cctccacgga caaatcattt aaccctttgg taaaagcttc
     4921 tcctttacta ggtacgttac taccatagcc aaaccaaacc attgaaaaaa tgacaacgaa
     4981 gagaaatcga ataaacacca tttttgcgta atgtttccct ctaaatatac gaagagtaaa
     5041 tagatataac acatacaaga cgacaatccc cgcaccaata ggatcgtata tttttgaata
     5101 aatagtatcc gaaaaagtga aaaactggtg aattaactcg ttcattgcag ctccttcata
     5161 gagcttctca atgacaaaat caaggccttg ccaaatcaat ttacttaaac taaagaacat
     5221 attcgtcatc atttttagaa aatctgtgcc atttgcccca aataacattt tgcctaatga
     5281 aaattcacta tcagataaat aagaagaaaa ggtatcaata tgaaatttcc caatctcata
     5341 gatcgattga tccacctgct tttctgccgc atacaccaat ccaccaacaa aaagcagtcc
     5401 gaatccaatc gttaaaaaaa acaaaacgca ccatttcttt tccttatgct ttatataagc
     5461 cattgtttca cctcatttct attgcatgat taacgtatcc tgttcttcta cttgagagtg
     5521 acttgttttt tccgttgttt gtaatgcagc ggtcatttct tcaaacaaac tgtgtacatt
     5581 gagttttcct acacgtccat agggatctaa catccaacac tgtcctttaa tcatgtggct
     5641 catttcagct aagttcgctt catttacttc caaacgtaaa tgattcaaaa tcttttctcg
     5701 ctcactgtct tcatcaaaag caaagacgac accaaaattc cctttttctt tatctgaacc
     5761 taagtcacca ataaattgtg tatctaacac taattgattc atctgggaac gccccacacg
     5821 tgccattgct gcaataatac tacgtcctgc gttcgaatta ttaaataccc atgcttctgt
     5881 aaaataaatc gctgttttct tactactatc tcgttttcca aacatttcac aaaaacgacc
     5941 taaactaatc atcaaacaca aagactttcg atccgcatct gtatatagtt ctggtcggac
     6001 attgtcattt ggtaatttca aatcttggat ctctaaaatc gttgttttgg cattaaagtc
     6061 taatcccggg ttttctccat aagaaaatcc taaacgtaaa atcgaatctt ctaccgtcaa
     6121 gcgtaaaaac tcaccatatt ctcgaacctc tttttgttcc atttgttcta aatagtcgac
     6181 cacatgcatc aagccaactt gttgtccggc ttctttttga actaaaactt cttggattcc
     6241 ctttaatacc gcccctttta aaaggtgatt ggtacctaac ggacttaact catcaatcat
     6301 atcctgggct acttgtttcg catctactcg ggataaacaa acaattggat ctaatacacc
     6361 ataattacgg ggatttgtag catctaacgt gacataactc attttttgca aatgttctac
     6421 aaacaaaggg taatgtttct gataatacgg gtcttgaatg agtttcgtaa accatttgcg
     6481 cttttctcct ttgggatcca caaataaact ttgtacattc ataaacgaac tatacaaaaa
     6541 gagcaagcca actaaaaagg tcttcccttt accggttttt ccagttactg ctacatgagg
     6601 cgcatcaaac aacgcacctt tgattccttc cgcaatggct agaagattca aaaagacaaa
     6661 acgatttgac gcataaatta attcttctaa tggtgttttt tctcctcgag aaccaccatc
     6721 ccctaatgta tcttgccgac caatatacca gccggattcc atacctaatt ctgatactgc
     6781 tgaaaacaac cattcgccaa ctgcttctac agttgttcgg tgcttccaat gtaactgccg
     6841 tcccaatggt tgccctaata aacatttata gaacaattct cgttgatcat tttttgcacg
     6901 gtggatatgg ataatcgtat gaagttgttg aataaccgtt gcactatttt gacgaacttc
     6961 ttcaaccgtt tgaccgtata caataaaaca tgcgatccaa tcgacgacag gttgcccatt
     7021 atctactcgt tgctttaaat gttttactaa tcctcgaaca gttaataagc gttcactagg
     7081 ttcttcatca ttttgaacca attccttatg ctctgttttg aaccgttccg atagccaatt
     7141 cagttttaaa ccaatacctc cctgtccttt caagcgtgga tagtgaattt tcattcgcaa
     7201 ttctgctggg aaagaaagat cctgagcaaa ttgaaataga tcacaatttc tcatattttc
     7261 aggtgtttcc ccaataggca acaatgctaa ataactactc cctaactcat cttctaattg
     7321 aagaaggcct ttatgttcct tcggtatacg tacatacgtt ccttgattgt ctttcagtac
     7381 ttcccaatta ttttgttctt ctactttgtg agccattcct cgaatatatt gcagacgatt
     7441 gagatacaac agctcttttt ttactaaggg acgaatagaa aagctataaa gtaattgtgt
     7501 cacttctttt acataaggat cactttcaga aaacaaagtt tccaaatcat ctacttctaa
     7561 tccaaataag cgtgctaaac ttcctgctaa atctaaccag attagattta ttacatcatg
     7621 aaaactctcc accgttgctt tttctttgac tttgattcct aaaataaatg aaggacgata
     7681 gacttgacct tctcgttcca acaatgtcac tgtttctttt gcataatacg caccaattgc
     7741 ttgactatcg ggagaaaatc cctttcctaa tgtgtcaaac ttcgtccgta aattcattgt
     7801 tcgtggatac atcaccaact ccacatcttc ataatctgct aaaagcaaca gtaaacgagc
     7861 catcatttct tttatttcct gtgtcttttt tggtgtgttt tttgaaattg ttcgtggaaa
     7921 aacatgatag taggcccaga tacaatcctc tccatttaac aaacaatttt tcttgaatgc
     7981 ttttactggt tcttttacaa gcaccaatga atttcacctc ccaataaaaa aggaacaacc
     8041 agttgattgt ccctttttct aatcctctaa ataaacatac ttattcacaa aaagtaattc
     8101 ttccaatgca ttgtttgata ccttgtccca gttataatac acacaatcac cattcatcat
     8161 acgaatcata taacaaaacc aatcccataa ataaaaatgc actttttttc catcaggttt
     8221 tagttttgcc agtgctaaca cacataaata ggggagtcct atataaaaaa caactcctgc
     8281 tggtggaaaa aaagaaatga cccaaccaat aaatgaacgt aaaaataaat attcaaagat
     8341 tacgaccaac aaaaaagttg ctgcattgac taatgcaact ggaaatggaa gacgccaatt
     8401 gcttgtaatc tgttgtacgc gataaggttc cgcataaaca atcgaataat cataaggttc
     8461 ccaatccact tcactatcct cctaatccat aaataatttt tcaatgattt taccaaacgc
     8521 atcaaataca cgtgttggat catttaagaa gaaataaact gcggcacctg ctgcgataga
     8581 tattaatgca tttcctactt ttgatttcat aaaatttcgc aatcccagat aaacaaccac
     8641 cattattaaa aaatagccac cttgcgttaa tataaaatct acaattccct tcagtgtcat
     8701 ttccgttctt ctcctttcct atccaaccaa ttatgtgtta atttttcaac ataatactga
     8761 tttgtttttt tcgtaatgac aatcgtcatt tgttcttttt gtaaaagtga cgtatcacca
     8821 tctcgaaaga cgaccgttgc ttttacaata aaaggttttt tcgtttcttt ataacgcaat
     8881 actttcatct ctacttgatc aactttagct gtttgattga gtccttcagg ttctttcatc
     8941 atgtatgaca tatcttttgc attgtttttc gcatacttat ctaagaaatc ttccacaaat
     9001 ccttcaacat ctttcgcttc cgacgcatca accgtttcgt ataatgtgtc atcataagta
     9061 atcattcgtt gtttttctgt attctttggt agtgccgtaa aataaggata agcaataata
     9121 cgacagccct tgtttgaaat agaaaaggga agattgagta cttgtgtgat tgtcacactt
     9181 gtttctttct tactctctgg attcatcgag cgaatatcat aagttacttt ataactagca
     9241 gttggaatgg tttcaatttc ttttaatcca ataaacgaag cagcagttaa ttttcgaaca
     9301 cctgaaacac ttcctggttc ttgaataaag ttttgtgcat aaaactcttt ttttaatgtt
     9361 tcttgacgtt gttgttcttg ttcatgatta tcgaatgtat tcatatacaa agcaacaaaa
     9421 cgattcataa aggattcaat ttcaggtgtg tacgttaatt ctgaccgact ttgtacttct
     9481 ttcttttgta attgttggat ttcatctttc aaaaacgaca cttgaacctt cgttttcatg
     9541 gattgaaaaa atgccaagcc ccctgaaaaa caaagaaata aaaccaaaca ccaaatccat
     9601 ttcactgctt tttttgaatg gattttcttt tgcttcggta ctttctctgt ataaactttt
     9661 tctttccgcc gtagttgtcg ttttttcata agctactcct tctagtatgt ttttactagt
     9721 acgtacgatt gacaacgata agcttgtttt tctgaaaaat agtccattca attaccctca
     9781 ttttctattc cttttctctt tcttttatat ccaacaaatc cagcaatact tgtcaacact
     9841 cctcctagaa cagaaagtgc aacatgtatt tcttcaccag tctttggtaa gacttttgtt
     9901 tttccatctg ctgttttgat ttcaatattg ccacttggaa gtttctttac aggaatcgac
     9961 aaatccttta ccaaattccc ttcactatct tggattaatg gaataccatc ttttacacca
    10021 acaattgttt gatcacctgc tacttttaat ggggtggttg gtgttggaat atgggtcaat
    10081 tcctctacat tactagtgac aatcggtaca ctcattcctt gagaagtatt cgtcgtaatt
    10141 tcaccgcttg gggttaccga tactgtgact tcttttgctg gaacaatagg tttattttct
    10201 tttggaattg cttctgattt ttcaacaggt tgtttcggtt cttctttttg ttcttcactg
    10261 gtagacgaca tcgttggctg ttccgtactt gacgaatcag tattcttttc agaagaactg
    10321 gttgttcctg attgttccgg agttgtgact atacttgaat ccgttgttgt tgaactatgc
    10381 acaccttctt cttcgatcga atcagtcggt aaagttgatt caggaaactc tggttctggt
    10441 gtcactggct ctttttcagg tttctctgac tgcaattgtt caggttctgc tggtttttcc
    10501 gttgttcctc catttagctc tgtctcaaca tctggtaatc cttcccctgg caatgtttct
    10561 acaatttctg acttaggtgt ttctacagtt tcatctgcat aaaccaatga actccctaat
    10621 tgaaacgaaa ataatgaaat tcctagtgtt gttacgactg ctaatgttgt ttttttcatg
    10681 attattcctc caatttttta tgcaattcgt gttttgttca tagacgccgc actaatatta
    10741 tctgaacgat aaaagaactc ccgtccgttt tcttctaacc caacatatgg attatttttg
    10801 aataattgaa tcaatagttc actggtgttg tattttggat agtttgcaaa aaaataatac
    10861 tgtgcttgtc gcccatccga aaattcaaca agtaattcat aacgataccg cttcaatcct
    10921 tttcgttgtt ttaaaggatc tctataccaa cttgtccatc gttccattcg tggatagaga
    10981 tagacacgat acacatagaa caatgccata ccacatacca ccaaaaataa taatcgccac
    11041 ataaaaattc ccccttttct tctacctttc ttgaatcatc gaacattcat accctctata
    11101 gagatttgaa aaatccatca tcaattgttc atataaacgt tgttgcaaat agcgattgtt
    11161 tcgaatacat aattcttcat agccatcaat cacttgatct tctttttcat agcatcttgt
    11221 agctctatga cgctcagctt gcaagacata accaaactct gcaccatatc gttctactgt
    11281 taataactgt tgactacgca ataatgcaat ataacaatca caaaaatgtt caaccgacat
    11341 ttgatttttc tcacgtttgt gaaataggta ttctttagaa agcacattat tcgttaaaca
    11401 atctgataaa tagcgataat ctggcggtcg gttattttca tctgtgaatt tttcccataa
    11461 taaactgatt tttattcgtt cagcggctag atagtcttgt tcgcgtggat aaaattcatc
    11521 aatcgcacaa agttttttat cacctgaata aacaaatcca tataatgcga aacgaactct
    11581 tccttttcaa ttggctctcc taaaaattcc agtgacatac catcttctcc ttcctaaaaa
    11641 aaataaaaaa gcagaaaccg atttggcttc tacttttctt tccttttata gctctttttt
    11701 acggaagaat ccgcaaatga cattttttca aaaaatcaat cggcacaagt tcacgaaaac
    11761 ttggagcaat tttggataga atcacttccc catcttgtaa aatacaatca taaaaatcat
    11821 cagttacttg atacttacgc ttcttacaat aattgataaa atagtagcga tcattttcta
    11881 tttccattcg ttgttctact aagcgcatac gaacatcatc atagaaataa gccacaaatc
    11941 gcccttcaag ttcatgtttt tcaatgggat actcttctgg taacaatcta ttttttcgtc
    12001 gtttatacat tgctggatac cttagaagaa gttcatctaa acgtctgtca gtatatggta
    12061 actgacgaat caacgattct aatcgttctt tatacagata gaataattgg cgcaagcaat
    12121 agtgtaccgc ctctggattt tctaatactg ttggatcacg tgaccaatac gcaccaacat
    12181 aaatcataat ttttttcatt tagacaatca caccagcaat actaatgacc gatccacaat
    12241 aggtagaaca agtggcatag cgaccaattt cctctaattc tatctcaatc tcttctaaag
    12301 aaagatatgt ctgttcttct gttggctggc gactcatttc ccaagtgaca gtcaccaatg
    12361 aaatttcttc cataagaggt ttttcgctgc taagtgctgc cacacagttt tcatatatct
    12421 tcattttatc gcagcctttt ttaaccgcta aaatcgcagg tattggcaat gaaaatcgat
    12481 aaaagcgaat gaattctagc cttcctttcc ttcggctaaa ttccaactct tcgagttctt
    12541 gttcgtatcc cttacgaact tctaataagc gtgcaatttc ttgccagttc tctttttcct
    12601 gataacgtcc agcttttacc gcttgttcaa tataaggaac tcgaaaacga aaataagaca
    12661 aatcaaccat cttttcattt aatgcttcga ttgcagcctt taaatccaac aggcgcagcc
    12721 attctgttgt cgtatatgca ttcatgcaat tttccctcct gacgttccca tccattctag
    12781 atagttttgt tgctcgtcat ttagttcggc attatcaatc ccttcttgta caaagtctct
    12841 tcctaaaata cgatctgctt cttgtaacaa ctttaatgtc ggagctactt gtttcaaaaa
    12901 ccagcgttct gaacgatcta atgagaattt ttctggcttt actgtaaaat ccaattcttc
    12961 catcgaacga aataattttg cccatcgttt atcaacaatc cgtgcatcac tttccagtct
    13021 atcccaaaca ataagttttg cattaattaa accaacgcca atctctccta aacgtttcgg
    13081 tgaatcacct aaaataaatg cttctactgc ttgtgttgct ttctcattgg ctaaccgtaa
    13141 ctcatatcga ttataaatac catatttcgt taatgcttct aattctgaaa ttttttctcg
    13201 gttcgcaatt tcaaaacgtt tctcataaaa attaaaaaag agaggactct ttcgcgatcc
    13261 aaaatacaaa gataatcctt cacttcgaaa atagccatca tctaaaaaac aaccgccaat
    13321 attctggaat ctcttaaagt ccatttctaa taaacctgct ttttgttttt ctagtaattc
    13381 aaagagatcg aaatgttctt ccgtttccaa tccccaaagt tcatctaacg caatatcgaa
    13441 tcggctacat tgcacacgtg tataaccagc ttccaacttt ccttgatttt tccaaatgga
    13501 atatagccat gttcgccaat ctgtttgttg ttcacgcatg acttcctcat attcccggca
    13561 accttgtcct gaaaattgca caagtacatt ttctgttaca gttcgttttg aatgccagaa
    13621 cacccagata ttcccacgaa cagaaagaca gtcaaaaaga tacaagcccg ttggacgtgt
    13681 ttcaaattct gtcagatcca ttcgtaatac acgttgaatc actgttttgt agtctgtccc
    13741 aaaaaaggaa agacgcaaat aatcaatcat tgctcgaaga tctattccgc ttttcatata
    13801 caatcgaaag cgttccttga ttcgttgttc aaaatcctct gggatttttc ttttcccttg
    13861 ttccatcttt ctaatgtaat cttttgagca gcccatcaat tgcgcaaatc ttgcttgtga
    13921 tagcggcttt ttatctccac ttatattatg aattgcactt aacttctttc tgtagctttt
    13981 tagctcaatc ggtgtcattt tctcgctcct tttataaaat aacaaaatcg atttttttta
    14041 accctttaga aacaccgata tatcaactag tccccctcgt tttttgggaa aagtagtaca
    14101 actgctgacc ttagataatc acttgataca aaagggttta taaaactttt ttctatgtgt
    14161 tatgttattt ctgcccccca tattatatat cggggggctt tacacaaaga ggccgtgcgc
    14221 aagcgcacga cctctttgtg agctgcacac gctgctagcg agtgcgcatt ataggcccat
    14281 gcttcgcagc cttaatgcgc accgccaggc agagtgcggc taccagacta cgtctagagc
    14341 cctcccgata gataataact acaaaattaa atgatctaat tttgttattt cactatattt
    14401 ttcaggagaa aaaacgcga
//
LOCUS       DAZKLD010000072        13984 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.072, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000072 DAZKLD010000000
VERSION     DAZKLD010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13984)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13984)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13984
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.072"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..357)
                     /locus_tag="ACG8KR_001724"
     CDS             complement(<1..357)
                     /locus_tag="ACG8KR_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HFQ0201091.1"
                     /translation="MEPEQLRAIVKENNSLETKLTKRNQQYIFDLKKSLEAANLSEEE
                     KVKALHDILPTLVAEQKGGRTARQLFGTVSERTEAIINKPAEVKNSSTPLLMWLDNTL
                     LLLGLLSIMIAITGLFS"
     gene            complement(447..1547)
                     /gene="ychF"
                     /locus_tag="ACG8KR_001725"
     CDS             complement(447..1547)
                     /gene="ychF"
                     /locus_tag="ACG8KR_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002269933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HFQ0201092.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDWRLQRLTELVHPKKTVPTTFEFTDIAGIVKGASKGEGLGNQFLSHIRQVDAICH
                     VVRCFDDDNITHVEGRVDPLEDIDTINLELVLADLESINKRYTRVAKVAKTKDKDAVA
                     ELAVLDKIKPVLEEGKSARTIEFTEEEQKIVKSLFLLTTKPILYVANVSEDDVADADA
                     NHYVQEVRNFAAEENAEVIVVSARAEEEIAELDEEDKAEFLEALGIEESGLDQLIRAA
                     YDLLGLATYFTAGEQEVRAWTFKKGIKAPQAAGIIHSDFERGFIRAETVSFEDLDHYG
                     NMHAAKEAGRVRLEGKDYVVQDGDVMLFRFNV"
     gene            complement(1556..1747)
                     /locus_tag="ACG8KR_001726"
     CDS             complement(1556..1747)
                     /locus_tag="ACG8KR_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HFQ0201093.1"
                     /translation="MYDLHDIVEMKKPHACQTNRWEVIRMGADIKIRCMNCGHIVMMT
                     RRDFEKKLKKVIEKNAEKE"
     gene            complement(1768..2658)
                     /locus_tag="ACG8KR_001727"
     CDS             complement(1768..2658)
                     /locus_tag="ACG8KR_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HFQ0201094.1"
                     /translation="MKKSKGLGRGIDALFQDLENLETVDVKDDTVVQLPLNELRPNPY
                     QPRKTFEEVSLQELANSIEQSGVFQPIIVRRSAVKGYEIIAGERRFRASKLAEKETIP
                     AIVRDFDEEAMMQVAVLENLQREDLNPLEEAEAYEMLMKNLKLTQAEVASRLGKSRPY
                     IANYLRLLSLPDLVKEMVQDQRLSMGQARTLLGLKNKTLILKLANRAVTENLTVRQLE
                     QLVSELNQNDGKEKKKIPRLAKEKPYYIRESEDRLMDKFGTSVAIQEKNGKGKIEIEY
                     LSQKDLTRILDILNIQFDEE"
     gene            complement(2645..3412)
                     /locus_tag="ACG8KR_001728"
     CDS             complement(2645..3412)
                     /locus_tag="ACG8KR_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HFQ0201095.1"
                     /translation="MAQIISVANQKGGVGKTTTTVNLGACLASLGKKVLLVDMDAQGN
                     ATSGVGIRKPDVTRDIYDVLVNELPIDEATLITEHENLSIVPATLQLAGAEIELTSMM
                     ARESRLKGSLAEVSSQYDYILIDCPPSLGHLTINSFTASDSILIPVQCEYYALEGLSQ
                     LLNTVRLVQKHFNPELEIEGVLLTMYDARTNLGNEVVEEVRKYFREKVYETIIPRNIR
                     LSEAPSHGKPIIDYDPRSRGAEVYQALAKEVVSREEK"
     gene            complement(3424..4137)
                     /gene="rsmG"
                     /locus_tag="ACG8KR_001729"
     CDS             complement(3424..4137)
                     /gene="rsmG"
                     /locus_tag="ACG8KR_001729"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HFQ0201096.1"
                     /translation="MIPEEFRQSLAEKGIELTDHQMNQFAIYYRTLVEWNEKMNLTAI
                     TDEKDVYLKHFYDSLTLAFEVDFTQPLSLCDVGSGAGFPSIPLKIAFPELQITIVDSL
                     NKRITFLSELTRLLGIDASLYHDRAETFGQNPHFRASFDLVTARAVARLNVLAELCLP
                     LAKQEGYFYALKAAKSEEELTEAKQAIALLGGKFLKEEAVTLPNVSDQRHIIVIQKKK
                     PTPKKYPRKPGLPNKQPIK"
     gene            complement(4369..6270)
                     /gene="mnmG"
                     /locus_tag="ACG8KR_001730"
     CDS             complement(4369..6270)
                     /gene="mnmG"
                     /locus_tag="ACG8KR_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HFQ0201097.1"
                     /translation="MEHYEADAYDVIVVGAGHAGSEAALAAARMGSKTLLLTINLDMV
                     AFMPCNPSVGGPAKGVVVREIDALGGEMGKNIDKTYIQMRMLNTGKGPAVRALRAQAD
                     KHAYSTEMKHTIEKEPNLTLRQGIVEELIVEDGVCHGVVTSTGASYRAKAVVITAGTA
                     LRGEIIIGELKYSSGPNNSQPSIGLANHLKELGFAIERFKTGTPPRVKSSTIDYSVTE
                     EQPGDKEPNHFSFSTPDSAYKLDQESCWLTYTGETTHQIIRDNLHRAPMFTGIVEGVG
                     ARYCPSIEDKIVRFADKPRHQLFLEPEGLNTEEVYVQGLSTSLPEDVQVNMLHSIAGL
                     EKVEMMRTGYAIEYDVVEPHQLRPTLETKVVENLYTAGQTNGTSGYEEAAGQGLIAGI
                     NAALKVQGKEPLVIKRSDGYIGVMIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLR
                     LTEMGHRVGLVKEDQYKNYLKKKEAVEQEIKRLMNVRIKPTEEVQDFLATINAAPLKD
                     GVLASDFLRRPEITYAELLRFIPENEELTRKEIEQVEIQIKYEGYIKKAMEKVEKLKR
                     MEAKKIPENIDYAAINGLATEAKQKLQKIQPETIAQASRISGVNPADLSILMVYIEQG
                     KIAKVSEPA"
     gene            complement(6287..7684)
                     /gene="mnmE"
                     /locus_tag="ACG8KR_001731"
     CDS             complement(6287..7684)
                     /gene="mnmE"
                     /locus_tag="ACG8KR_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HFQ0201098.1"
                     /translation="MAEITLEFDTIAAISTPPGEGAISIVRLSGDQAVQLADKVYQSG
                     NKRLSEVPSHTIHYGHIVDPKSNQLVDEVMVSVMRAPKTFTREDVVEINCHGGIVVVN
                     QILQLLLREGARLAEPGEFTKRAFLNGRVDLSQAEAVMDLIRAKTDKAMGLALNQLDG
                     NLSALIRSLRQEILETLAQVEVNIDYPEYDDVEELTTKLLLEKAQMIQQRIQALLATS
                     KQGKVLREGLSTAIIGRPNVGKSSLLNHLLREEKAIVTDIAGTTRDVIEEYVNVRGVP
                     LKLIDTAGIRETEDVVERIGVERSRKALAEADLILLVLNQSEPLTAEDEQLLEATSGL
                     KRIILLNKTDLPAQLEQEKLKKLIENEPVFSISVAKNDGLDRLESAISDLFFSGETGE
                     RDATYVSNTRHIALLEKASLSLEEVIAGIDSGMPVDLVQIDMTRCWDYLGEVVGDSVQ
                     DELITQLFSQFCLGK"
     gene            7983..8366
                     /locus_tag="ACG8KR_001732"
     CDS             7983..8366
                     /locus_tag="ACG8KR_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0201099.1"
                     /translation="MVALSKLYTFERRKYVSPFRSAALEEESDPRINDVVLSTGNINQ
                     KYIVRDIVFVVECLEADLFDPSFPKDDLFLTTKRKLKTKVLEYGANAVINCHFEYQQK
                     KRDGKYFLELFAYGTVIQFIQTTIG"
     gene            complement(8534..9292)
                     /gene="jag"
                     /locus_tag="ACG8KR_001733"
     CDS             complement(8534..9292)
                     /gene="jag"
                     /locus_tag="ACG8KR_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding cell elongation regulator Jag/EloR"
                     /protein_id="HFQ0201100.1"
                     /translation="MPIYEAATIDEAVQLGLADLGLAKDQVEIEILEEGKKGFLGMGR
                     KNARLSIQPSISEKVSETVEQTVEEILEEEPTAEIEVVNETILEEKTEEVSELGDEAA
                     LTELAVYLTEISKQLDAPALVKMERESNNMVVFHLETQKQGILIGKHGKTLNALQYLA
                     QVFIHRLARNKLSVVVNVGNYREKRQEIIQRLADRTAEKVKRTGQPVFLEPMPAFERK
                     QIHAALSKDEHVKTHSEGEEPYRYLVVEPAKKYY"
     gene            complement(9310..10128)
                     /locus_tag="ACG8KR_001734"
     CDS             complement(9310..10128)
                     /locus_tag="ACG8KR_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HFQ0201101.1"
                     /translation="MKKYKRLLLMAGIVSLVIVLSGCGTGEVSAQSTGIWDRYIVYYF
                     AEAIKALSFGNAGIGIILFTLIIRVILLPLMHFQTKSMRKTQELQPKLKALQQQYSSR
                     DPETQSKLREEQQRLYAENGVNPYAGCLPLLVQMPILMALWQSISRVPELKQGHFLWL
                     NLGNADPTFILPILAAIFTFASTYLSSMSQIESNASMKIMNFVMPVMILVMGVNLASG
                     LSLYWVVSNAFQVVQTLLINNPFKIRKEREEEARRIRERERALKKAMNPKKKRK"
     gene            complement(10125..10481)
                     /gene="rnpA"
                     /locus_tag="ACG8KR_001735"
     CDS             complement(10125..10481)
                     /gene="rnpA"
                     /locus_tag="ACG8KR_001735"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HFQ0201102.1"
                     /translation="MRKAYRVKKEREFQAVFHTGDSFANRKFVVYRLEKPGQPHFRVG
                     LSVGKKVGNAVNRNEVKRKIRAAVYSIKEEIDPELDFIVIARPSVKGLTYDEVRSNLV
                     HVLKLAKIIDREEKSK"
     gene            complement(10599..10733)
                     /gene="rpmH"
                     /locus_tag="ACG8KR_001736"
     CDS             complement(10599..10733)
                     /gene="rpmH"
                     /locus_tag="ACG8KR_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HFQ0201103.1"
                     /translation="MKRTYQPNKRKRQKVHGFRKRMSTKNGRRVLASRRRKGRKVLSA
                     "
     gene            11256..12590
                     /gene="dnaA"
                     /locus_tag="ACG8KR_001737"
     CDS             11256..12590
                     /gene="dnaA"
                     /locus_tag="ACG8KR_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HFQ0201104.1"
                     /translation="MVSLDALWNELKATYQKDLSPASYNTWIETANPRTLDQNQLVVE
                     VPSKIHKEYWEKNLATKIVEVGYMLSGNEIIPRFITGEEAEQEEVIEEKNPKVVAPSP
                     LKKAMLNPKYTFDTFVIGKGNQMAHAAALVVAEDPGSIYNPLFFYGGVGLGKTHLMHA
                     IGHQMLQSQPNAKVKYVSSETFANDFINSIQNKTAEEFRQEYRNVDLLLVDDIQFFAE
                     KEATQEEFFHTFNALYNEGKQIVLTSDRLPNEIPKLQERLVSRFAWGLSVDITPPDLE
                     TRTAILRKKAAAERLEIPDDTLSYIAGQIDSNIRELEGALVRVQAFAAMNSEDISTSL
                     AADALKTLKSGKGHPQLSILQIQEEVAKYYHIQLKDLKGKKRVKSIVVPRQIAMYLAR
                     ELTDNSLPKIGAEFGGKDHTTVIHAHEKIQQLMDSSVSMQNEVSEIKNFLLN"
     gene            12788..13918
                     /gene="dnaN"
                     /locus_tag="ACG8KR_001738"
     CDS             12788..13918
                     /gene="dnaN"
                     /locus_tag="ACG8KR_001738"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HFQ0201105.1"
                     /translation="MKVTLNRASFMQELQTVQRAISSKTTIPILTGVKITLTQEGLTL
                     TGSNADISIETFLSVENEKANMQIESTGSIVLQARFFSEIIRRLPEETFTLEVLENKQ
                     VAITSGKANFIVNGLDADNYPHLPVVESHNQMKLPVHVLTKLINETVFAVSQHESRPI
                     LTGVHFILSDNSLLAVATDSHRLSQRVIPVEQAADHFDIVIPGKSLIELSRSLTNEEE
                     IVEISIMENQVLFKTETMYFYSRLLEGNYPDTNRLIPSSFNTEVEFSVPSFLAAIERA
                     SLLSHEGRNNIVRLSIRPDAVVLYGNSPEIGKVEESLSYTASSGDPLDISFNPDYMKA
                     ALRAFGDMSIKVKFISAIRPFTLEPTEDGVQFIQLITPVRTN"
BASE COUNT         3873 a         2951 c         2498 g         4662 t
ORIGIN      
        1 tgaaaacaat ccggtaatcg caatcatgat acttaataga ccaagcaaca gcaacgtatt
       61 atctaaccac atcaataaag gggtggatga attttttact tctgctggct tattgatgat
      121 tgcttccgtt ctttcagaga ctgtcccaaa caattgacgg gctgttcttc cgcctttttg
      181 ttctgcaact aaagtaggca gaatgtcatg aagggctttt actttttctt cttcagacaa
      241 attcgctgct tctaaagatt ttttcaaatc aaaaatgtac tgttgatttc ttttggttaa
      301 ttttgtttct aaactattgt tttcttttac gattgctcgt aattgttctg gttccattat
      361 ctaatagtcc tccttagccg ttccttttct caacggtcgt cacaaaagac attctaccat
      421 attctacagc tagtttgata gcccttttac acattaaagc ggaataacat tacgtcgccg
      481 tcttgtacga cataatcttt tccttctaat cgaacacgtc cagcttcttt tgctgcatgc
      541 atgtttccgt aatgatccaa gtcttcaaaa gagacagttt ctgcacggat aaatccgcgt
      601 tcgaaatctg aatggatgat tcctgctgct tgtggtgctt tgattccttt tttgaatgtc
      661 caagcacgta cttcttgttc cccagcagta aagtaagtag ctaagcctaa caaatcataa
      721 gcagcacgga tcaactgatc caaacctgat tcttcgattc ctaaagcttc taaaaattcc
      781 gctttgtctt cttcatctaa ttctgcgatt tcttcttctg cacgagcact gaccacgatc
      841 acttctgcat tttcttctgc agcaaagttg cgcacttctt gtacataatg attcgcgtct
      901 gcatctgcta cgtcatcttc tgagacattg gctacataaa gaattggttt tgtcgtcaat
      961 aaaaatagtg atttaacgat tttttgttct tcttcagtaa attcgatcgt tcgggcagat
     1021 tttccttctt ccagtacagg tttgatttta tccagtacag ctagttctgc cactgcatct
     1081 ttatctttgg ttttagcaac ttttgctaca cgagtatagc gtttgttgat agattctaag
     1141 tctgctaaca ctagttccaa gttgatcgta tcaatatctt ctagtggatc aacacgtcct
     1201 tctacgtgag taatattatc atcatcaaaa cagcgaacaa cgtgacaaat agcgtctact
     1261 tggcggatat ggcttaagaa ttgatttccc aaaccttctc ctttacttgc gccttttacg
     1321 attccagcaa tatctgtaaa ttcaaaagtc gtcggaacag tttttttcgg atgtacaagt
     1381 tctgttaagc gttgcaaacg ccagtcaggt acttctacca tccctacgtt ggggtcgatc
     1441 gtcgcaaagg gatagtttgc tgcttctgct cctgcttttg tgatcgcatt aaacaaagtt
     1501 gattttccta cgttgggcaa cccaacaatt ccagcagtca atgccataaa tttattcact
     1561 ctttctctgc atttttttca attacttttt ttaatttctt ttcaaaatct cgacgggtca
     1621 tcatgacgat atgtccgcaa ttcatacatc tgattttgat atctgcgccc attcgaatga
     1681 cttcccagcg attcgtctgg caagcgtgag gttttttcat ttcaacgata tcgtgcaagt
     1741 cgtacataaa aatagcctcc tttttgttta ttcttcatca aattgaatat tcaagatatc
     1801 aagaattcga gtcaaatctt tttgagaaag atattcaatc tcgatcttac ctttcccatt
     1861 tttttcttgg atagctacac ttgtaccaaa tttatccatc aaacgatctt cgctttctcg
     1921 gatataatat ggtttttcct ttgctagcct tgggattttt ttcttttctt ttccatcatt
     1981 ttgatttaac tcagaaacaa gctgttccag ttggcggaca gttaaatttt cagttactgc
     2041 acgattggct aatttcaaga tcaatgtttt attttttaaa cctaacagcg tacgggcttg
     2101 tcccatagat aaacgctgat cttgtaccat ttccttgaca agatccggta aagaaagcaa
     2161 gcgcaaataa ttggcgatat aaggtctgct ctttcctaaa cgactagcta cttctgcttg
     2221 tgttagtttc aggtttttca tcagcatctc atatgcttct gcttcttcta acggattcag
     2281 atcttctcgc tgcaagtttt ccagcactgc tacttgcatc atcgcttctt cgtcaaagtc
     2341 tctaacgata gcagggatcg tttctttttc agctagtttg gacgctcgaa aacgtcgctc
     2401 ccctgcaata atctcatatc cttttactgc ggaacggcgt acgatgatgg gttgaaagac
     2461 acctgattgt tcaatagaat tagctagttc ctgcaaagat acttcttcaa atgttttacg
     2521 gggctgatac ggatttggtc gcagctcatt caatggtagc tggacaacgg tatcatcttt
     2581 tacatccacc gtctctaagt tttctaaatc ttgaaacagt gcatcgattc ctcggcctaa
     2641 tcctttactt ttcttcacga cttaccactt cctttgccaa tgcttggtag acttcagctc
     2701 ctcgagaacg tggatcgtag tcgatgatcg gttttccatg actaggtgct tctgataaac
     2761 gaatatttct tggaatgatc gtttcgtaaa ctttttcgcg gaagtattta cgtacttctt
     2821 ctacgacttc atttcccaga tttgttcgtg catcatacat tgtcagtaag actccctcga
     2881 tctctaattc tggattaaag tgtttttgga ctaaacgaac cgtattcagt aattggctta
     2941 atccttccaa tgcgtaatac tcacactgaa caggtatcaa aatcgaatca cttgctgtaa
     3001 atgaattgat tgtcagatgg ccaagagaag gcgggcaatc gatcaaaata taatcatact
     3061 gactgcttac ttctgctaaa gatcctttta atcttgattc tcttgccatc attgacgtca
     3121 attcgatctc tgctcctgct aattggagag tcgcaggcac aatactcaaa ttttcgtgtt
     3181 cagtgatcaa agttgcctca tcaattggca attcattgac tagaacatca tagatgtctc
     3241 ttgtcacatc aggttttctg atcccgacac cactggtagc atttccttgt gcatccatat
     3301 ccactagcaa tacttttttg ccaaggctag caagacaagc acctagattc accgtcgtcg
     3361 ttgttttgcc gacgccacct ttttggttcg caacagatat tatttgtgcc atctcttctc
     3421 ctcctatttg attggctgct tattaggcag tccgggcttt cttgggtatt tctttggggt
     3481 tggtttcttt ttttgaataa cgatgatatg ccgttgatca gagacattcg gcaaggtaac
     3541 cgcctcttct ttgaggaatt taccacctaa taaagcaatc gcttgttttg cttctgtcaa
     3601 ttcctcttca ctttttgctg ctttcaatgc ataaaaatag ccttcttgtt tagccaaggg
     3661 caaacacaat tccgctaaca cattcaaacg agcaaccgca cgagcagtga caaggtcaaa
     3721 tgatgcacga aagtgaggat tttgcccaaa tgtttctgcc cggtcatgat ataaggaagc
     3781 atcgattcct aataatcggg ttaactcaga taagaaggtt attcgtttat tcagagaatc
     3841 aacgatcgtt atctgcagtt ctggaaaggc aatttttaaa ggtatgctag gaaaccctgc
     3901 ccccgaacca acatcgcata aagataaagg ttgcgtaaag tctacttcaa atgctaatgt
     3961 aagtgaatcg taaaaatgct tcagatatac gtctttttca tccgtaatag ctgtaaggtt
     4021 catcttttcg ttccattcga ctaatgtccg ataataaata gcgaattgat tcatttgatg
     4081 atcggtaagc tctatccctt tttctgccaa ggattggcga aattcttctg gtatcataaa
     4141 tattcctttc attgtgaaac agcaattgtt tcacagattc tctccctact ttaacgcaaa
     4201 atctctagac atgcaatcct tccatatgga ttcttaaaaa tttgaaaagt aagaaaagcc
     4261 gatagacttt ttctttctag tatgactcat aaaatataca cagaagagag aactagcttt
     4321 gagcaatttc tgcaaaaact agttctctct tctatttatt attacgtatt acgctggttc
     4381 actgactttc gcaatcttcc cttgttcgat atagaccatt aggatactta ggtctgcagg
     4441 gtttacgcca ctgattcggc tagcttgggc aattgtttct ggttggattt tctgcaattt
     4501 ttgttttgct tctgttgcta atccgttgat tgctgcgtag tcgatgtttt ctggaatctt
     4561 tttcgcttcc atgcgtttta atttttctac tttttccatg gcttttttga tgtagccttc
     4621 atatttgatt tggatttcta cttgttcgat ttcttttcgt gtcaattctt cattttctgg
     4681 aataaaccgt agaagttctg cataagtaat ctcaggacgt cttaaaaagt cgcttgctag
     4741 tacaccatct tttaatggtg ccgcattaat cgttgctaaa aagtcttgga cttcttctgt
     4801 tggtttgatt cgtacattca tcaaacgttt gatttcttgt tcaactgctt cttttttctt
     4861 caaataattc ttgtattgat cttcttttac taagcctaca cggtgaccca tttctgttaa
     4921 tcgcaaatct gcattatcat gacgtaaaag cagacggtat tcagcacgtg aagtcagcaa
     4981 acgataaggt tcgttcgttc cttttgttac caaatcatcg atcatgacac cgatgtaacc
     5041 gtcacttcgt ttgatcacta aaggttcttt tccctgaact ttcaaagctg cattgatacc
     5101 ggcaatcagc ccttgtccag ccgcttcttc ataaccgctt gtaccatttg tttgtccagc
     5161 tgtataaaga ttttcaacga ctttagtttc taatgtcgga cggagctggt gtggctctac
     5221 tacgtcatat tcgatagcat atcctgtacg catcatttct actttttcta atccagcaat
     5281 agaatgaagc atgttgactt gaacatcctc tggtaaagaa gtagataaac cttgaacata
     5341 gacttcttcc gtatttaagc cttctggttc caaaaataat tgatgacgtg gtttgtctgc
     5401 aaaacgcacg atcttatctt cgatggatgg acaatagcga gcgcctaccc cttcaacgat
     5461 ccctgtaaac attggggcac gatgtaagtt gtctcgaata atttggtgag tcgtctcacc
     5521 tgtataggtc agccagcaag attcctgatc tagtttatag gcactatctg gtgtgctgaa
     5581 gctaaaatga ttcggctctt tgtcaccagg ctgctcttct gtaacagaat agtcgatcgt
     5641 acttgatttt actcgaggcg gtgttcctgt tttgaaccgt tcgatagcaa agccaagttc
     5701 ttttagatga ttcgctagtc caatagaagg ttgagagtta ttcggacctg atgaatattt
     5761 caattcaccg ataatgattt ctccgcgaag tgctgttccg gctgtaataa caactgcttt
     5821 ggcccggtag ctcgctcctg tggacgtcac tactccatga caaacaccat cttcgacgat
     5881 cagttcttcg acgattcctt gtcttagcgt caaatttggt tctttttcga tcgtatgttt
     5941 catttctgtt gaataagcat gtttgtctgc ttgtgctcgc aatgctcgga cagctgggcc
     6001 ttttcctgtg ttcaacatac gcatttggat ataggtttta tcgatattct tacccatttc
     6061 tccgcctaac gcatcgattt ctcgaacgac aacgccttta gctggtccgc ctacagaagg
     6121 attacatggc atgaatgcta ccatgtccaa atttattgtc agtaataatg tttttgatcc
     6181 catacgtgca gcagcaagtg cagcttctga accggcgtgt cctgcgccaa caacgatcac
     6241 gtcatacgca tctgcttcat aatgttccaa tcgtttgttc ctccttttat tttcctaaac
     6301 aaaattgact gaataattga gtgattagct catcttgtac actgtcgccg acaacttctc
     6361 ctagatagtc ccagcaacgc gtcatatcta tttgaacgag atccacaggc attcctgaat
     6421 caatacctgc aatgacttct tcaagtgaga gggaagcttt ttctaacaac gcgatatggc
     6481 gggtattaga aacataagta gcgtctcttt ctcctgtttc tccgctgaag aataaatcag
     6541 aaatagctga ttccaaacga tctagtccgt cgtttttagc tacggaaatt gaaaaaactg
     6601 gttcattttc gatcaatttc ttcaattttt cttgttccag ctgtgctggc aaatctgttt
     6661 tatttagaag aatgatccgt ttcaatccac ttgtcgcttc caataattgt tcatcttctg
     6721 ctgtcagtgg ttcgctttgg tttagaacaa gtaagatcaa gtctgcttct gctaatgctt
     6781 ttcggcttct ttcgaccccg atccgttcca ccacatcttc tgtttcccga attccagctg
     6841 tatcgattag cttgagcggt accccccgaa cattgacata ctcttcgatt acatctcgag
     6901 tcgttcctgc aatatccgta acgattgctt tttcttctct taacaaatgg tttaacaaac
     6961 ttgatttgcc gacatttggc cggccgatga ttgccgtact taacccttcc cgcagaacct
     7021 tcccttgctt ggatgtagca agtagcgctt ggattctttg ttggatcatc tgcgcttttt
     7081 caagcaataa cttcgtagtc aattcttcga catcatcata ttctggataa tcaatattta
     7141 cttctacttg tgctaatgtc tctaaaatct cttgtctcaa tgagcggatc aaagcagaca
     7201 gattgccgtc taactgatta agagctagtc ccattgcttt gtctgttttt gcccgaatca
     7261 aatccatcac agcttctgcc tgtgaaaggt caacacgacc attcaagaat gctcgttttg
     7321 tgaattcgcc tggctcagct aatcttgcac cttctcttaa caatagctgc aaaatttgat
     7381 tgactacaac gatcccaccg tgacaattga tttctactac atcttcccgc gtaaatgtct
     7441 ttggtgctct catcacggag accatgactt catcgacaag ctgattgctc ttgggatcaa
     7501 cgatatgtcc ataatggatc gtatgacttg gtacttctga taatcgtttg tttccagatt
     7561 gataaacttt atctgctagt tgcacagctt gatcgccact taaacgaacg atactgattg
     7621 caccttctcc aggtggggta gaaattgccg caatcgtatc aaattctaaa gtaatttcag
     7681 ccattttttc acttcctttt tcattttgcg cacaaaaaaa gtgcccactc caccctttat
     7741 gcacaataaa cggtgaactt cgcactttga ctgcttgttc ttgattaaat tctggtcata
     7801 gattatcaca cttgctataa ggaatcaagt gtttaaaaca attagatgaa gttgagcctg
     7861 ttctgcttcc attaccttat atttaattgt ccttggatgt ctctttgcgt tctttggttt
     7921 attcgaagag aagatactgt ataaacaagg ctttatcttt cttaaaaaaa acggtacact
     7981 aaatggtagc gctatctaaa ctatacactt ttgaaaggag aaaatatgtg agcccgttca
     8041 gatctgctgc attggaagaa gaaagtgacc ctcggatcaa cgatgtcgtt ttatctacag
     8101 ggaatatcaa ccagaaatat atcgtccgcg atatcgtttt tgttgtggaa tgcttagaag
     8161 ctgatttgtt tgacccatct tttcccaaag atgacctttt cttaacaacg aagaggaaat
     8221 taaagacgaa ggtacttgaa tatggtgcga atgcagtaat caactgtcat tttgagtatc
     8281 agcaaaaaaa acgcgacgga aagtattttc tggaactttt tgcttatgga acagtgatcc
     8341 aatttatcca aacaacgatt ggttaaatga aacagacaaa aaaagcggat caaaactgaa
     8401 gacagtttcg atccgctttt ttctatcaaa gttagtgact acttcatcgt atactctgat
     8461 aagaatgatt tctagtaaaa tatacttggt ttgaaaaact ttgtcaacgt gatttattca
     8521 aaaagtaatg gaatcaataa tattttttag caggttctac tactaaataa cgataaggtt
     8581 cctcaccttc cgaatgtgtt tttacatgtt catctttgct taatgcagca tggatttgtt
     8641 tacgttcaaa tgcaggcatt ggttctaaga aaacaggctg ccctgtccgt tttacttttt
     8701 cagccgtgcg atcagctaaa cgctgaatga tctcttggcg cttttcacgg taattgccta
     8761 cattgactac tacagacagc ttatttcttg ctaaacgatg gatgaagact tgtgccagat
     8821 attgcaatgc attcagtgtt tttccatgtt tcccaatcag tatgccttgc ttttgtgttt
     8881 ctaaatggaa aacaaccata ttatttgatt ctctttccat tttgacaaga gctggagcat
     8941 caagctgttt tgaaatttca gtaagataaa ctgccaattc cgttaaagct gcttcatcac
     9001 ctagctcact tacttcttct gttttttctt caagaatcgt ttcgttgact acctcgatct
     9061 cagctgttgg ttcttcttcc aaaatttctt ctacagtctg ttctactgtt tcagaaactt
     9121 tttcgctgat acttggctgg atcgaaagcc gtgcattctt tcgacccatt cctaggaaac
     9181 ctttttttcc ttcttccagt atttcgattt cgacttggtc tttggccaaa cccaaatcag
     9241 ccagacctag ttggacagct tcgtctattg ttgcagcttc ataaatcggc attagcatta
     9301 cctcccctat tatttccgtt ttttcttagg attcattgct ttcttaagag cacgttctct
     9361 ttcgcggatt ctccgtgctt cttcttcacg ctcttttcga attttgaaag ggttgttgat
     9421 cagcagcgtt tgtacaactt ggaaggcatt ggaaactacc cagtataatg aaagaccgct
     9481 ggctaaattg acacccatca ctaaaatcat tacaggcatc acgaaattca tgatcttcat
     9541 tgatgcattc gattcaattt gactcatgct agataaatac gtacttgcaa aagtaaagat
     9601 tgctgctaaa atcggcaaaa taaatgttgg atccgcgttc cctaagttca accataagaa
     9661 atgcccttgt ttcaactctg gtacacgtga aatagattgc cataacgcca tcaagattgg
     9721 catttgtact aataaaggaa gacaaccagc atatggattg acgccatttt ctgcatataa
     9781 acgctgctgc tcttctctta acttactttg tgtttccgga tctcttgaac tatattgttg
     9841 ctgcagtgct tttaatttcg gttgtaattc ttgcgtttta cgcatgctct ttgtttggaa
     9901 atgcatcaac ggcaatagga tcacgcggat gatcagtgta aataaaatga tccctatccc
     9961 tgcattacca aatgacaacg ccttgatcgc ctcagcaaag taataaacaa tataacgatc
    10021 ccaaattcct gtactctgtg cgctgacttc cccagttccg caaccggata agacgatgac
    10081 caaactaacg ataccggcca tcaaaagtaa acgtttgtat ttcttcactt actcttttcc
    10141 tctctgtcga ttatttttgc aagttttaag acgtggacta aattggagcg aacctcatca
    10201 taagtcaacc ccttcacact tggtctagca atcacaataa aatccaactc cggatcaatt
    10261 tcttctttta tagaataaac tgctgctcgg atttttcgtt tgacttcatt gcggtttacc
    10321 gcgttgccga ccttttttcc tacagataaa ccaactcgaa aatgaggctg tcccggtttt
    10381 tccaaacggt aaacgacgaa tttgcggtta gcaaaagaat ctcccgtatg gaacacggct
    10441 tgaaactctc gttctttttt tactcgatac gcttttctca ttagttttct ttccttttct
    10501 atctctcact tacgctcata atcataccat aattacgatt gtcctttcat catttcccta
    10561 aaaaagagta taaaaaaaac cactgaaacc ccagtggcct aagctgaaag aacttttctt
    10621 cctttgcgac gacggctagc taacacacga cgtccatttt tagtactcat acgtttacgg
    10681 aatccgtgaa ctttttgacg tttacgttta tttggttggt atgttctttt catatattcc
    10741 acctccaaaa tgttcttatc tattttataa acacagacat actttgatag tataacgaca
    10801 aagtagtgat tttgtcaata taaaatagaa gatcgattgt taaaacttat ccacaagatt
    10861 atttttttct atgggaatcc tatgtggaaa aagttatgca cagggattat ccacagataa
    10921 taaaaggctg gttttacaca gaaaacacac gtgtaaaaaa agtaatccac aatcaggcga
    10981 tttcctgtgt attccgtctc ctaactattg gtatatctgt attttttatc cacagacgca
    11041 agaggaaatc tattggtttt tacgagtttt ccacctgtgc attagtggtg tggaaaactt
    11101 tttttgacaa ctgtgttttt ctcttttttc tttttgtcga ttgtgtggat aattgtaatt
    11161 aaaaaatttt tttcttcggt ttctgtggaa aactattgaa aaaaatgcta aattagtact
    11221 agttattcac tttttaatga aggaggaggc cttatatggt atccctcgat gctttatgga
    11281 atgaattaaa agcaacatac caaaaggatt tatcgcctgc tagctataat acatggatcg
    11341 aaacagcgaa ccctcgaacc cttgatcaaa accagctcgt tgttgaggtt cctagcaaga
    11401 ttcataaaga atattgggaa aaaaacctgg cgacaaaaat cgttgaagtc ggttatatgt
    11461 tatcaggaaa tgagattatc cctcgcttta ttactggcga agaagcagag caagaagaag
    11521 ttatagaaga aaaaaatcca aaagttgtgg cacctagtcc gctaaaaaaa gccatgctga
    11581 accctaaata tacctttgat acgtttgtca ttggtaaagg aaaccagatg gctcatgcag
    11641 cagcgctcgt tgtagcagaa gatccaggtt ctatttataa cccgctcttc ttttatggag
    11701 gcgtgggact tggtaagact cacttgatgc atgcaattgg tcatcaaatg ctgcaaagtc
    11761 agcctaatgc caaagtaaaa tatgtaagca gtgaaacttt tgctaatgat ttcatcaatt
    11821 cgatccaaaa caaaacagca gaagagttcc gtcaagaata tcgaaatgtt gatttactgc
    11881 tagtggatga cattcaattt tttgcagaaa aagaagctac gcaagaagag tttttccata
    11941 ctttcaatgc cttgtacaat gaagggaaac aaatcgtact aaccagtgat cggttgccaa
    12001 acgagattcc gaaactgcaa gaacgccttg tttctcgctt tgcatggggg ctgtctgttg
    12061 atattacacc gccggatctt gaaacaagaa cagcgatttt acgtaaaaaa gcggcggctg
    12121 aacgtctaga gatcccagat gacacactaa gctacatcgc aggacaaatt gattctaaca
    12181 ttcgtgaatt agaaggtgcg cttgttcgag tgcaagcttt tgctgcgatg aatagtgaag
    12241 atatttcgac tagtctagct gcagacgcac tgaaaacgtt aaaatcagga aaaggacatc
    12301 ctcaattgtc aatcctgcag atccaagaag aagtggcaaa gtattatcac atccaattga
    12361 aagatctcaa aggaaagaaa cgcgtcaaat ctattgttgt tcctagacaa atcgctatgt
    12421 atttggcacg tgagctcaca gataattctt taccgaaaat cggagctgaa ttcggtggca
    12481 aggatcatac aacggttatc catgctcatg aaaaaatcca gcagctgatg gattctagtg
    12541 tcagtatgca aaatgaagta tcagaaatca aaaacttttt actaaattaa tttgtcgatt
    12601 ctatcaactg aaaatagcat gtggataaaa gaaaaaaacg ttcaaaagtt atacacactt
    12661 tttccacagc tgttttttcg tttctctctt acgttttttg agttttccac agaatcaaca
    12721 tgccctatta cttttattat tctttttaac taataataaa taaacaaaca ttcaaaggag
    12781 atacatcatg aaagttactt taaaccgagc tagctttatg caggaattgc aaactgttca
    12841 acgagctatt tcaagcaaaa ccacgatccc tattttgaca ggtgtaaaaa tcacactgac
    12901 acaagaaggt ttgactttga cggggagcaa cgctgatata tcaattgaaa cttttttgtc
    12961 tgttgaaaac gaaaaagcaa atatgcaaat cgaatctact ggttccattg ttttacaagc
    13021 acgtttcttt agcgaaatca ttcggagact tcctgaagaa acatttactt tagaagtttt
    13081 agaaaataaa caagtagcga tcacttctgg aaaagcgaat tttatcgtaa atggattaga
    13141 tgcagataac tatcctcatc ttcctgttgt cgaaagccat aaccagatga aattacctgt
    13201 acacgtattg actaaactaa tcaacgaaac agtttttgct gtctcacaac atgagagtcg
    13261 tccaatcttg acaggtgtgc atttcatttt atctgataat tctttattag ctgtagctac
    13321 tgattctcac cgtctaagtc aacgcgtgat tccagtagaa caagcggctg atcattttga
    13381 tattgttatt cctggaaaaa gtttgatcga attatctcgc tcattaacaa atgaagaaga
    13441 aatcgtcgaa atcagcatta tggaaaacca agtgttgttc aaaacagaaa cgatgtattt
    13501 ctattcccgt ttgctagaag gaaactatcc agataccaat cgtttgattc cttcaagttt
    13561 taatacagag gttgaatttt ctgttcctag ctttttagcg gcgatcgaac gggcttcttt
    13621 actttctcat gaaggccgta acaacatcgt tcgtttatct attcgtccag atgcggttgt
    13681 tttatacgga aattcgccag aaatcgggaa agtagaagaa agcttgagtt atacagcaag
    13741 cagtggcgat ccattagata tttctttcaa tccggattat atgaaagcag cgttgcgtgc
    13801 atttggagat atgagcatca aagtgaaatt tatctctgct attcgtccat ttacattgga
    13861 accaacggaa gatggcgttc aatttattca gctgattact cctgtacgta caaattaaca
    13921 atgattttca atgttcttga aaatttaatt gaaaagaaat agaaaaaata aaattttaaa
    13981 caga
//
LOCUS       DAZKLD010000073        13969 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.073, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000073 DAZKLD010000000
VERSION     DAZKLD010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13969)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13969)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13969
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.073"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(325..2322)
                     /gene="tkt"
                     /locus_tag="ACG8KR_001739"
     CDS             complement(325..2322)
                     /gene="tkt"
                     /locus_tag="ACG8KR_001739"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFQ0201106.1"
                     /translation="MFDKIDQLGVNTIRTLSIEAVQKANSGHPGLPMGAAPMAYALWT
                     KHLKVNPTTSKNWADRDRFVLSAGHGSAMLYSLLHLAGYQVTIDDLKQFRQWDSKTPG
                     HPEVHHTDGVEATTGPLGQGIAMAVGMAMAEAHLAATYNKENFNVMDHYTYAICGDGD
                     LMEGVSQEASSMAGHMKLGKLIVLYDSNDISLDGPTSKAFTENVGARYEAYGWQHILV
                     KDGNDLEAISKAIEEAKAETDKPTLIEVKTVIGYGAPKEGTSAVHGAPIGEDGIKMAK
                     EVYGWEYPDFTVPEEVAARFHQTMIEEGQKAEDAWNEMFANYKKAYPELAQQFEDAFD
                     GKLPENWDAELPTYEVGSSQASRVSSKEVIQELSKAIPSFWGGSADLSGSNNTMVAAD
                     KDFTPEHYEGRNIWFGVREFAMASAMNGIQLHGGTRIYGGTFFVFVDYLRPAVRLAAI
                     QHTPVVYVLTHDSVAVGEDGPTHEPIEQLASVRCMPGVQVIRPADGNETRAAWKVAME
                     TTDAPTILVLSRQNLPVLPSTKEVADEMVKKGAYVLSPSQGETPEGILIATGSEVDLA
                     VKAQKELAEKGKDVSVVSMPSFDLFEKQSSEYKESVLPKSVKKRVAIEAAASFGWERY
                     VGTEGATITIDHFGASAPGTKILEEFGFTVENVVNTYNQLS"
     gene            complement(2424..2675)
                     /locus_tag="ACG8KR_001740"
     CDS             complement(2424..2675)
                     /locus_tag="ACG8KR_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HFQ0201107.1"
                     /translation="MLSPEKIERINELARKKKENGLSKAEEQEQINLRKEYLEAFRSG
                     MRHHIEGMKVVDPEGNDVTPEKLKEIQKKKGLHNRNDNF"
     gene            2822..3445
                     /gene="lexA"
                     /locus_tag="ACG8KR_001741"
     CDS             2822..3445
                     /gene="lexA"
                     /locus_tag="ACG8KR_001741"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HFQ0201108.1"
                     /translation="MARQTESRQIEVLRYIHEQVTQKGYPPTVREIGEAVQLSSTSTV
                     HGHLARLEKKGFIQRDPTKPRAIELTKQGLEKIGIRPTTIPVLGVVTAGEPILAVEDA
                     SDFFPIPPNLSSEEGSLFMLTIRGDSMINAGILDGDNVIVRKQETAQNGDIVIAMTAE
                     DEATCKRFYKEKNFFRLQPENDLLEPIILDQVSILGRVVGLYRSHIY"
     gene            3624..3842
                     /locus_tag="ACG8KR_001742"
     CDS             3624..3842
                     /locus_tag="ACG8KR_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0201109.1"
                     /translation="MKSMRRLGLVLTVLFLGIIIGNFGIRPALLVFVPLFTLWFMLWD
                     EKKYRQSERRRIHEDHFYHEPYYRSRQS"
     gene            complement(4130..4642)
                     /locus_tag="ACG8KR_001743"
     CDS             complement(4130..4642)
                     /locus_tag="ACG8KR_001743"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HFQ0201110.1"
                     /translation="MDLKDYIASIPDYPSKGIVFRDISPLMADGDAYREATKQIVNYA
                     KEKRIDMVVGPEARGFIVGCPVAYELGVGFAPVRKKGKLPRETIEVTYGLEYGTDTLT
                     LHKDAIKPGQRVLICDDLLATGGTIKATIELIEQLGGVVVGCAFLIELMDLHGRDKIK
                     GYDIITLMEY"
     gene            complement(4656..6953)
                     /gene="recJ"
                     /locus_tag="ACG8KR_001744"
     CDS             complement(4656..6953)
                     /gene="recJ"
                     /locus_tag="ACG8KR_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008409 - 5'-3' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HFQ0201111.1"
                     /translation="MRQANFDWKLSETEPSESFLQMIRELKLSPFVGKLLWQRGYHEE
                     KDINRFLHPKEQELHDPYLMHDMDKAISRIQEAVISGEKILVYGDYDADGITSATVMK
                     ETLELLGAEVEVFLPNRFEHGYGPNQSVYQEKIEEGIQLIITVDNGVAGNDAVAYAQQ
                     AGVDVIITDHHELPENLPDAYAIVHPRHPAGHYPFPDLAGVGVAFKVASALLEEPPVE
                     FLDLVAIGTIADLVSLTDENRILVSLGINAIHHSERIGLQALFEESGVKMRDADETTI
                     GFSIAPRLNAIGRMGDPRPAVSLLATFDEEEASVQAKKLNEINEERKAIVEQITQEAL
                     AMVNKENHIHLLVNRGWHEGVLGIVAGKIMNETGKPTLVLTLKEDGSAKGSGRSIEAL
                     NLFEMLDQMRDLFTYFGGHHAAVGLTMPSENVTILQEKMNQYIVDHQIDLMRGPELRI
                     DEVLLPNEVTVERIDELKLLAPFGTDNPLPQFLFRQVQAENIKRIGANQQHLKFVLSN
                     ASSQLDAVAFGFGTQEEELLNNQVDVVGKLSINEWNGRKKPQLMVSDFSVGGLQVFDW
                     RAKRFREQSRTSEGSLYLAFDSSTLKFVPEIIQEAAIVFEGLDSVKAIIEKRKPNSLV
                     IMDCPDDLADLKEVFRSYSFNRVYLMGISPDEAYLNGVGTREQYAKLFKLIHSQERID
                     IRHKIKAIAQYLRIPEKLLIFMIQVFFELKFVTIENGVLQKVASPESHPLTESRLYQR
                     RLNKIKVEEFLLLSDIPTIKKWLTT"
     gene            complement(7095..7538)
                     /locus_tag="ACG8KR_001745"
     CDS             complement(7095..7538)
                     /locus_tag="ACG8KR_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA
                     domain-containing protein"
                     /protein_id="HFQ0201112.1"
                     /translation="MKELKKQSNVILGFVLVLIIVLFAVLNNKNVPVSFGFTSFSAPL
                     ILVIIGSALIGALIVFLTASATLWQQKKQIKQLTQEMSEYQTELQKKIEEAKEEQQRE
                     FRNERAELEAAYQAELQAKTAQISQLKAPEEQKTQSPKQSFNYFD"
     gene            complement(7539..8327)
                     /locus_tag="ACG8KR_001746"
     CDS             complement(7539..8327)
                     /locus_tag="ACG8KR_001746"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HFQ0201113.1"
                     /translation="MNLEDKVVLVTGSSGGLGAQICYEAAKQGAIVISCARRMAFVEG
                     VRDECRRLSGKEAYAFKVDVSNPESVDELYEKVMEEVGRVDILVNNAGFGIFEDFLTF
                     DLGKAYDMFEVNILGMMVLTQKFAIDMAERRQGHIINIASMAGKMATAKSTVYSATKF
                     AVLGFSNALRLELKPLGVAVTTVNPGPIETDFFDKADPSGSYLEKVGQIVLEPAKLAK
                     MIVRNMRHPKREINQPFILEVASKFYTLFPTIGDYLASGIFNKK"
     gene            complement(8324..9265)
                     /gene="rnz"
                     /locus_tag="ACG8KR_001747"
     CDS             complement(8324..9265)
                     /gene="rnz"
                     /locus_tag="ACG8KR_001747"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HFQ0201114.1"
                     /translation="MELQFLGTGAGVPAKHRNVTSIALKLLDERNEVWLFDCGEGTQM
                     QILRTTIRPRKIGKIFITHLHGDHIFGLPGLISSRSFQGGDTPLEIYGPKGIEEYIKV
                     SLGISQTRLSYPLKFIELNETAPIFTDQQFSVYTKKLNHGIDSFGYRVVEHDHKGELQ
                     VDRLKELGIPAGPLYGKLKQGETIQLEDGRTINGKDFVGPDKKGRIVTILGDTRKTHN
                     SVVLAENSDILVHESTFNKDEARMAHNYFHSTTHQAAEVAKEAQAKRLLLTHISARYL
                     GKAALELEKEAQEVFENTTIMKDFDSIEIPFREEEEA"
     gene            complement(9442..10029)
                     /locus_tag="ACG8KR_001748"
     CDS             complement(9442..10029)
                     /locus_tag="ACG8KR_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HFQ0201115.1"
                     /translation="MSKRVIILNFQVESQAYQAFSEIKKLHAVRSIKGEQMAVVSHDP
                     NGTHQFKIEDFIDFTGNNKSSTGGLIGMLVGVLGGPLGILLGWFAGGMIGATQDAKEI
                     KDAQTVFDFVGNKIGEGATGLILIADEEDNRPLNQLVMMELGGEITRLDYEDVEKDVH
                     DAKEAENHAKEAAQKTWKDKHPQTSNDNESSGSSN"
     gene            complement(10250..11563)
                     /gene="obgE"
                     /locus_tag="ACG8KR_001749"
     CDS             complement(10250..11563)
                     /gene="obgE"
                     /locus_tag="ACG8KR_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003126661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HFQ0201116.1"
                     /translation="MSMFLDQVTIDVKAGKGGDGMVAFRREKYVPDGGPAGGDGGRGG
                     DVILIVDEGLRTLMDFRFNRHFKAQPGENGMSKGMHGRGSEDTYVKVPQGTTVRDAET
                     GALLGDLIENGQTLVVAKGGRGGRGNIRFASPRNPAPEIAENGEPGQERKIELELKVL
                     ADVGLVGFPSVGKSTLLSVISSARPKIGAYHFTTLVPNLGMVTTSDGRSFAAADLPGL
                     IEGASQGVGLGTQFLRHIERTRVILHVIDMSGMEGRDPYEDYLAINKELSTYNLRLLE
                     RPQIIVANKMDMPDAPENLVKFKEQLNKEKEDEFADDIPVFPISGVTRQGLDALLNAT
                     ADLLEVTPEFPLYEEELEEETVHYGFNPEGPEFQIDRDSDATWILSGEKIEKLFQMTN
                     FDHDETVMRFARQLRGMGVDEALRARGAKDGDLVRIGEFEFEFVE"
     gene            11851..12861
                     /gene="gap"
                     /locus_tag="ACG8KR_001750"
     CDS             11851..12861
                     /gene="gap"
                     /locus_tag="ACG8KR_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFQ0201117.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIKEVSDDIEVVAINDLTSPTMLAQ
                     LLQFDSTHGTYPGTVTATEDSIVVDGEATRVYAEPDASKIPWAKENGVDIVLECTGFY
                     TSEEKAKAHLDAGVKRVVISAPAGAMKTIVYNVNDDTLDENDKIISAGSCTTNCLAPM
                     AYFLNNEFGIEVGTMTTVHAYTSTQMLLDGPVKGGNLRAARSAADNTIPHSTGAAKAI
                     GLVIPELQGKLQGHAQRVPVVDGSLTELVSVLKTKVTADQVNEAIKKHTVDNPSFGYD
                     DRQIVSSDVIGTTQGSIFDPTQTEVTTAGDFQLVKTVAWYDNEYGFTCQMIRLLEKFA
                     NL"
     gene            complement(12946..13881)
                     /locus_tag="ACG8KR_001751"
     CDS             complement(12946..13881)
                     /locus_tag="ACG8KR_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFQ0201118.1"
                     /translation="MKLWMKILLGVALALIVALVFAGNYFYNYAVVPSEKDFLDEDTP
                     GTTQTSKETDAHKWFSSKENRSDWTITSKDGLKLSAIYLPAETKSEKTALVAHGYMGN
                     AETMTNYAKMFHDMGYNVLVPDARGHGKSEGDYIGFGWPERKDYVQWINKVLENNGKS
                     QEIVLYGVSMGAATVMMTSGEKLPDNVKAIIEDCGYSSVHDELAYQLDDMFSLPAFPL
                     MQVTSLVTKVRAGYFFGEANAVEQLKKNQRPMLFIHGDEDTFVPFEMLDKVYRATKGP
                     KEKYVVKGAEHAEAYKTDPEKYQQVVQQFLSQYVH"
BASE COUNT         3856 a         3130 c         2455 g         4528 t
ORIGIN      
        1 gagcaatcgc ttttttattg atcaccattt ttcttttagg tctatggtgt cgggcactgc
       61 gggtcttcag ctcttgcatt caaattcctc ctcaaaataa aaaaacttaa taaaaagttc
      121 tcttcttaaa acaaacttaa ttaattaaat tatactttta attctcaaga aacacaaact
      181 aatccgaaga attctttgtt ttgtcattta aatgtaacat agccgaaaac gaaaagtaat
      241 attgatttat tcttctaaca cataaagaac agctgtctcc aacttgcaga cagctgttct
      301 tatcagtcaa acaattgctt ggatctatga tagttgattg tacgtattca cgacattttc
      361 tactgtaaat ccaaattctt ctaaaatttt agtgccaggg gcagatgcac cgaaatgatc
      421 gattgtaatc gttgcgccct ctgttcctac gtagcgttcc caaccaaatg atgcagcagc
      481 ttcgatagcc acacgtttct tcactgattt aggaagtaca ctttctttat attcagaaga
      541 ttgtttttca aagaggtcaa agcttggcat agaaacaaca gaaacatctt tgcctttttc
      601 agccaattct ttttgagctt tcacagccaa atcaacttca gaacctgttg caatcaaaat
      661 accttctggt gtttctcctt gtgatggaga aagaacatac gcgccttttt tcaccatttc
      721 atctgctact tcttttgtag aaggcaatac tggaaggttt tgacgactca atacaaggat
      781 agtaggcgca tctgttgtct ccatcgctac tttccaagca gcacgtgttt catttccgtc
      841 tgccggacga atcacttgaa caccaggcat gcagcgaaca gatgctagtt gttcaatcgg
      901 ttcgtgcgtt ggtccgtctt ctccgacagc aaccgagtca tgtgttaaga cgtaaacaac
      961 aggagtatgt tggattgctg ctaaacgaac tgctggtctt agataatcga caaagacaaa
     1021 gaatgttcca ccgtagatac gagttccgcc atgaagttgg ataccattca tagccgaagc
     1081 cattgcaaat tcgcggacac cgaaccagat gttgcgtcct tcataatgtt caggagtgaa
     1141 atctttatct gcagccacca ttgtattatt ggaacctgac aagtccgctg aaccacccca
     1201 gaagcttggt atagctttcg ataattcttg gatgacttct ttacttgaaa cacggcttgc
     1261 ttgactgctt ccaacttcat aagttggcaa ttctgcatcc cagttttctg gtagtttgcc
     1321 atcaaaagca tcttcaaatt gttgtgctaa ttcaggataa gcttttttat aattggcaaa
     1381 catttcattc catgcatctt cagctttttg tccttcttct atcatcgttt gatggaagcg
     1441 ggcagctact tcttctggaa cagtgaaatc tggatactcc caaccataaa cttctttagc
     1501 catcttgatg ccatcttcac caataggagc accatgaaca gcagatgttc cttctttagg
     1561 agcaccatag ccgatgactg tttttacttc gatcaatgtt ggtttgtctg tttcagcttt
     1621 tgcttcttca atcgctttag agattgcttc taggtcattt ccgtctttca ctaagatatg
     1681 ttgccagcca tatgcttcat agcgagcacc aacgttttct gtgaaagctt ttgaagttgg
     1741 accgtctagt gaaatatcgt ttgaatcgta taaaacgatc aatttcccta gtttcatatg
     1801 acctgccatc gaacttgctt cttgagatac cccttccatc aagtctccgt caccgcatat
     1861 tgcatacgta tagtgatcca tcacattaaa gttttctttg ttatacgttg ctgcaaggtg
     1921 cgcttctgcc attgccatac cgacagccat tgcaatccct tgtcctaatg gccctgtagt
     1981 ggcttctaca ccatctgtgt gatgtacttc aggatgtcct ggtgttttag aatcccattg
     2041 acggaattgt ttcaaatcat cgattgtcac ttgatagcca gctaaatgaa gcaagctata
     2101 caacattgct gacccatgtc ctgccgaaag aacgaaacgg tcgcggtcag cccagttttt
     2161 tgatgtcgtt ggatttactt tcaggtgttt tgtccataac gcatatgcca taggtgctgc
     2221 gcccatcggc aatccaggat gtcctgagtt tgctttttgg actgcttcaa tacttaatgt
     2281 acgaattgtg ttaacgccaa gttggtcaat tttgtcaaac aaaataaccc ctcctattaa
     2341 tttaggtttt ttttaccttt atttcttaca ctttcattct ataagatgtc acacgaaaaa
     2401 gcaatctttt tcagtatttt ttactaaaag ttgtcatttc ggttgtgtaa gccttttttc
     2461 ttttggattt cttttaattt ttccggggtc acgtcattgc cctcaggatc gacgactttc
     2521 attccttcga tatgatgacg cataccggaa cgaaaagctt ccaaatactc tttgcgtaag
     2581 ttgatttgtt cttgttcttc ggcttttgag agaccatttt ccttcttttt acgtgcaagt
     2641 tcattgatcc tctctatttt ttcaggtgac agcatgctca gccctccttt tactctcctt
     2701 catattatat aaaaactgaa aaaaaaacaa ctagataaac taaataaaat acgaacattc
     2761 gtttgattta gcgcttttta tatggtacta tttatttatc aagtgaaaga aggtgtacct
     2821 tgtggcccga caaactgaat caagacaaat cgaagtctta cgatatatcc atgaacaagt
     2881 aacccaaaaa ggatacccgc cgactgtccg tgaaatcggg gaagctgttc agctttcttc
     2941 tacttctact gttcacggtc atcttgcacg gcttgaaaaa aaagggttta tccaaagaga
     3001 tccgacgaaa cctcgtgcaa tcgagttgac aaaacaaggt ttagaaaaga ttgggattcg
     3061 tccaacaact attccagtac ttggcgtcgt aacagctgga gaacccattt tagcagtaga
     3121 ggatgcttca gacttctttc caatccctcc taatctttct tccgaagaag gcagtctctt
     3181 tatgctgaca attcgtggag atagtatgat caatgctggg atcttggatg gtgacaacgt
     3241 gatcgttcgg aagcaagaaa ctgctcaaaa tggcgatatt gtcattgcaa tgactgccga
     3301 ggacgaagcg acttgcaaac gattctataa agaaaaaaac tttttccgtc tgcagcctga
     3361 aaacgacttg ctcgaaccaa tcatcttaga tcaagtatct atattaggac gagtagtagg
     3421 actttatcgt agtcatatct attaattgct actaataaaa gaaaactttt taaaaagagt
     3481 ttgagacgat ggtctcaaac tcttttttcg cccgggttct tcttctttca aatttttatc
     3541 acttccctct ttacaaaccg aacaaatatt cgtatactaa atatacgaac aattgttcgc
     3601 ataaattaac gaggtgagaa ttcatgaaat caatgagacg tcttggctta gtattaactg
     3661 ttttgttttt aggtattatt attggtaatt tcggcatacg tccagcttta cttgtgtttg
     3721 ttccgttatt tactttgtgg ttcatgttgt gggatgaaaa gaagtaccgt caatctgagc
     3781 ggcgcagaat tcatgaagat catttctatc atgagcccta ctacagatcc aggcaatcat
     3841 aataaggatt gactgctaat tattcaaaag acaagaagtt aaacttttac gggggtgtga
     3901 caaaaatcgg tcacatcctt ttttcgaata aacggtgttc taaagccagt ttttcggtat
     3961 taagtcaaat tgcggaaaaa gtgatttttt aaacttctac tggtctttgc ttgtttccat
     4021 ttataaaaaa atagcgaccc tcaaaagtca gatagagaat ctgacttttg agggtcgctt
     4081 taaaatcgct tctttaagaa taacatcaag aatagcttct tttttctgct taatattcca
     4141 ttaatgtaat gatgtcatat cctttgattt tgtcgcgtcc atggagatcc atcaattcga
     4201 tcaaaaatgc gcagcctacg actacaccgc ctaattgttc gatcagttcg atcgtcgctt
     4261 tgattgttcc tccagtagct agcaaatcgt cacaaatcag cacacgttga cctggtttaa
     4321 tggcatcctt atgcaatgtc aatgtatctg ttccatattc taatccataa gtcacttcga
     4381 tggtttctcg aggtaacttc ccttttttgc ggactggggc aaaaccgact cctaactcat
     4441 aagcaacagg acatcctacg atgaatccac gggcttctgg cccaactacc atatcaattc
     4501 gtttttcttt tgcataattt acgatttgct tcgtagcttc ccgataagcg tcaccatctg
     4561 ccatcagtgg ggaaatatct cgaaacacaa ttccttttga cggatagtcg ggaatactgg
     4621 caatataatc ttttaaatcc actctttgtt tcctcctagg ttgttaacca tttctttatg
     4681 gtaggaatat cgctcaaaag caaaaattcc tcaactttga ttttattgag ccgccgctgg
     4741 tataatctgc tctcagtcaa aggatgactt tctgggctag cgactttttg taaaacaccg
     4801 ttctctattg taacaaattt caattcaaaa aacacctgta tcataaaaat taataatttt
     4861 tcagggatac gcaaatattg agctattgct tttattttat gacgtatatc aattcgttct
     4921 tgagaatgga tcagtttaaa caattttgcg tactgttctc ttgtgcctac cccattcaaa
     4981 taggcctcat caggcgaaat ccccatcaga taaacacgat tgaatgaata agaccgaaaa
     5041 acttctttca gatccgctaa atcatctgga caatccataa taactagaga gttcggtttt
     5101 cttttttcaa ttattgcctt tacagaatct agtccttcga aaacaatcgc agcttcctgg
     5161 ataatctcag gcacgaattt caacgtagaa gaatcaaatg ctaaataaag gctaccttcc
     5221 gacgttcggc tctgttcacg gaaacgtttt gcccgccaat caaatacttg cagaccaccc
     5281 acagaaaaat cagataccat cagctgcggt ttcttacgcc cgttccattc attgatcgaa
     5341 agctttccta ccacatctac ctgattattc agcagttctt cttcttgagt accaaaacca
     5401 aaagcgaccg catcaagctg actcgatgca ttggaaagga caaatttcag atgctgttgg
     5461 tttgctccga ttcttttgat attttctgct tggacttgtc gaaataaaaa ctgaggcaaa
     5521 ggattatccg taccaaatgg cgccaatagt ttcagctcat caattcgctc tactgttact
     5581 tcatttggca gcaatacttc atcaattctc aactcaggcc ctctcatcaa gtcaatttga
     5641 tgatcgacta tatattggtt catcttctcc tgtagtatag tgacattttc acttggcatc
     5701 gttaaaccga ctgctgcatg atgtcctccg aagtaagtaa ataaatctcg catctggtca
     5761 agcatttcaa aaagattcaa ggcttcaata ctacgtccag atcctttagc tgatccatct
     5821 tcttttaaag taaggacaag cgttggttta cccgtttcat tcattatttt cccagcaaca
     5881 atgcccaaaa cgccttcatg ccatcctcgg tttaccaata gatggatatg attctcctta
     5941 ttgaccatcg ctaatgcttc ttgtgttatc tgctctacga ttgcttttct ttcttcattg
     6001 atctcattca atttttttgc ttgaacggat gcttcctctt catcaaacgt cgcaagtaac
     6061 gagacggcag gacgtggatc gcccatccga ccgattgcat ttagtctggg agcaatcgaa
     6121 aaaccgatcg ttgtttcgtc tgcgtccctc atttttacgc cgctttcttc aaatagcgcc
     6181 tgaagtccta tccgttctga atgatgaatg gcattgattc ctaatgaaac aaggattcga
     6241 ttctcatcag tcaatgaaac taaatcggcg atcgttccta tcgctactaa atccaaaaat
     6301 tctacaggcg gttcttccaa taatgcagaa gctactttga atgctactcc gactcctgcc
     6361 aaatctggaa atggatagtg ccccgctgga tgacgagggt gaacaatcgc ataagcgtct
     6421 ggtaaattct caggtagttc gtgatgatct gtaatgatca cgtctacacc agcttgctga
     6481 gcgtatgcta ccgcatcatt tcctgctacc ccgttatcaa cagtgatgat caactggatt
     6541 ccttcttcga tcttttcttg gtatacagat tggtttggtc catacccatg ctcaaatcga
     6601 tttggcagga atacttctac ttctgcacca agtaattcca gtgtttcttt cattaccgtt
     6661 gcactggtga tcccatctgc gtcataatca ccgtaaacga ggattttttc tccactgatt
     6721 acagcttctt ggattcggct gattgcttta tccatatcgt gcatcagata cggatcatgt
     6781 aattcttgtt cctttggatg taaaaatcgg ttgatatcct tttcctcatg gtaacctctt
     6841 tgccacagta acttaccgac aaatggagat aatttcagtt cacggatcat ttggagaaag
     6901 gattcagaag gctctgtctc gcttaacttc caatcaaaat ttgcttgtct cactttctca
     6961 ctcctaactt agttattata gcaaaggaaa tcagcaaaaa aaagcagaaa gaaagaataa
     7021 aaacagatca gatccgctag acagatgttc agcaggacct gatcttttgt acgaccattt
     7081 atgaaatatc cctattagtc aaaataattg aagctttgtt tgggcgattg agttttttgt
     7141 tcttccggcg ctttcaattg actgatttgt gcggttttcg cttgtaactc cgcctgataa
     7201 gcagcttcca actccgcacg ttcgtttcta aactcgcgtt gctgttcttc ttttgcttct
     7261 tcaattttct tttgaagttc tgtttgatat tctgacattt cctgagtcag ctgtttgatt
     7321 tgcttttttt gctgccatag tgttgccgaa gcagtcaaaa atacaatcag tgcaccaatc
     7381 agcgcagaac cgatgatcac taagatcaat ggcgcagaaa aggaagtgaa gccaaaactt
     7441 acaggaacat ttttattgtt caatacagca aaaagaacaa taatcaaaac taaaacaaat
     7501 ccaagaatca cattgctttg ctttttcaat tccttcacct actttttgtt aaaaattccg
     7561 ctggctaaat aatcaccaat cgttggaaac agcgtataaa attttgatgc aacttctaaa
     7621 ataaacggtt gattgatttc ccgttttgga tgtctcatat tgcggacgat catttttgcc
     7681 aacttggcag gttctaaaac gatttgtccg actttttcca aatagcttcc gcttggatca
     7741 gccttatcga agaaatctgt ctcgattgga cctggattca ctgtcgtaac agctacgcca
     7801 agtggtttca attctaatcg caaagcgttg gagaagccaa ggactgcaaa tttcgtggca
     7861 gaataaaccg ttgattttgc agttgccatt tttccagcca tagacgcgat attaatgata
     7921 tgaccttgtc ttctttctgc catatcgatc gcaaattttt gtgtcaaaac catcattcct
     7981 aagatgttga cttcaaacat atcgtatgct tttccaaggt cgaatgtaag gaagtcttcg
     8041 aaaatcccaa aacccgcatt gtttaccaga atatcgacgc gcccgacttc ttccatcacc
     8101 ttttcatata attcgtccac gctttcagga ttgctgacgt ctaccttgaa agcataagcc
     8161 tctttgccgc tcaagcgtct gcactcgtct ctgacacctt ctacaaatgc cattcttcgt
     8221 gcgcaactta taacgattgc tccttgcttt gctgcttcat aacagatttg tgcgcccaga
     8281 ccgcctgaac tacctgttac taaaacaact ttatcttcta aattcatgcc tcttcctcct
     8341 cgcgaaatgg aatctcaatg ctatcaaaat ctttcatgat cgtcgtgttt tcaaacactt
     8401 cttgtgcttc tttttcaagc tctaaagctg cttttcctaa atatctcgca ctgatatgag
     8461 taagtagcag ccgttttgcc tgcgcttctt ttgcaacttc agcagcttga tgagtcgtgg
     8521 aatggaaata gttgtgcgcc attcttgctt catctttatt aaatgtgctt tcatgtacca
     8581 gaatatcgct attttccgca agcacaacgg aattatgtgt ttttctcgta tcgcctaaaa
     8641 tagtcacgat tcgtcctttt ttatctggac ctacgaaatc ttttccattg atcgtccgcc
     8701 catcttctag ttggatcgtt tcaccttgct tcagtttgcc gtacagtggt ccagctggaa
     8761 tacctaattc ttttagacga tccacctgca attctccttt atgatcatgt tcaaccacac
     8821 gataaccaaa actgtcgatc ccatgattta gtttttttgt ataaacagaa aattgctgat
     8881 cagtaaaaat cggggctgtt tcatttagct caatgaattt cagcggataa cttaaacgcg
     8941 tttgcgaaat acctaaagag acttttatgt attcctctat cccttttggc ccgtaaatct
     9001 ctaatggcgt atccccacct tgaaatgacc ggctgctgat gagcccggga agtccaaaaa
     9061 tatgatcgcc gtgtaaatgt gtaataaaaa ttttaccgat ttttctcgga cgaatagtcg
     9121 ttcgcaaaat ctgcatttgc gtgccttcgc cacagtcgaa caaccaaact tcattgcgct
     9181 cgtctaaaag tttcaatgct atacttgtca catttctatg ttttgcagga acacctgcac
     9241 ctgttcctaa aaattgtaat tccataatct acctgacttt ctttaagttc ctctttaatt
     9301 ttagggtaaa aagaagggat aatcaaccgt aagtcgagaa tgggttcaaa ttaaaaataa
     9361 cgttctaaag cgatagcgtt ttctttgaaa attactataa aaacagcagc gaacccgttt
     9421 gagatcctct gctgtttttt attaatttga agatccagaa ctttcattgt cgttggaagt
     9481 ttgcggatgt ttgtccttcc aagtcttttg tgcagcttct ttcgcatgat tttcagcttc
     9541 tttcgcatca tgcacatctt tttcgacatc ttcataatcc aagcgggtaa tttctccgcc
     9601 taattccatc attaccaatt gattcagtgg gcgattgtcc tcttcatcag caatcaagat
     9661 cagtcctgta gctccttcac cgattttatt tcctacaaag tcgaacacgg tctgggcatc
     9721 ttttatttct ttggcatctt gtgtcgcacc gatcattcct ccggcaaacc atcctaataa
     9781 aatccctagc ggaccaccta atacaccaac caacattccg attaatccac ccgtagaaga
     9841 tttgttatta ccggtaaagt cgataaaatc ttctattttg aattgatgtg tcccattagg
     9901 atcatggctg acaactgcca tctgttctcc tttaatcgaa cgaacagcat gtaacttttt
     9961 gatttctgaa aatgcctgat atgcctggct ttctacttga aaatttaaaa tgattactcg
    10021 tttgctcatt aaaaacgccc ctttcatacc ttcttgttat ttattttacc tttaattgta
    10081 aaataagaaa agaaaagcac tctttttttc caataaaata gggctgtgac aaaagtcgta
    10141 tcacagctcc tttttccgaa taaacggcgt tcttgaagct gatcacttct gtcacaacct
    10201 cccttaaatc tgaatctata ttttttggta tttgttttta tttgcttgcc tattcgacaa
    10261 attcaaattc aaactcaccg atacgtacaa ggtcgccatc tttcgcaccg cgtgcccgaa
    10321 gagcttcatc cacacccata ccacgtaatt gacgagcaaa acgcatcact gtttcatcat
    10381 gatcgaaatt cgtcatttgg aataattttt cgatcttctc accagataag atccacgttg
    10441 catctgaatc acgatcgatt tggaactcag gaccttctgg attgaatcca tagtgtacag
    10501 tttcttcttc cagttcttct tcgtacaatg ggaattctgg cgttacttct aacaaatctg
    10561 ctgtagcatt caataacgcg tccaatcctt gtcgagtcac tccagaaatt ggaaatactg
    10621 ggatgtcatc agcaaattca tcttcttttt ccttgttcag ctgttctttg aatttcacta
    10681 gattttccgg tgcatccggc atatccattt tgtttgccac gatgatttgc ggacgctcta
    10741 gcaagcgtaa gttataagta gataattctt tgttgatggc aagatagtct tcataaggat
    10801 ctcgtccttc catcccactc atatcgatca catgtaaaat tactcgtgtt ctttcgatat
    10861 gacgaaggaa ttgtgtaccc agcccaacac cttgcgaagc tccttcaatc aagcctggca
    10921 agtctgctgc ggcaaaactt ctgccatcac ttgtagtaac cattcctaga ttagggacga
    10981 gcgtcgtgaa atgataagcg ccgatttttg gacgagcaga tgaaataacg gatagtaaag
    11041 tcgatttccc gacagatgga aaacctacaa gtccaacgtc tgccaatact ttcaattcta
    11101 gttcgatttt tcgctcttgt cctggttcgc cgttttcagc aatttccggt gcaggattcc
    11161 gaggagaggc aaaacggata ttccctcgtc caccgcgtcc accttttgcc acaactagtg
    11221 tttgaccatt ttcaattaaa tctcctaaaa gagcaccagt ctccgcatct cggacagtgg
    11281 ttccttgtgg tactttaaca taggtatctt ctgaaccacg accgtgcatc cctttactca
    11341 tcccattttc acctggctgc gctttgaaat ggcgattgaa ccgaaaatcc atcaatgtac
    11401 gtaacccttc atcgacgatc aagatgacat cgccgccacg accaccgtct ccgccagccg
    11461 gtccgccgtc aggaacgtat ttttcgcgac gaaatgcaac cattccgtct ccgcccttgc
    11521 cagctttcac atcgatcgtt acttgatcta aaaacatgga cataattaag tcctcctatt
    11581 attcatttat ttatttgact gcctgaattt ccacaataaa caaaagtctt acttatttta
    11641 tcggtgaaat ctgattttgt ctatcatttc agcaaaaaga ttgtttacct tcttcttttt
    11701 ttatcatttg gtaaggtttc caaccttttt tctgctacca agtataattt tttgacaaaa
    11761 tattctttgc gtagcagaat aacagtgtat taattgtatt aattgaggat gccgtttcac
    11821 agcaccccta ataaaggagg aacttttgat atgacagtaa aagtaggtat taacggattt
    11881 gggcgtattg gccgtctggc tttccgccgc atcaaagaag tctcagatga tattgaagta
    11941 gtagcaatca acgacttaac aagtccaact atgttagcac aattattgca atttgactct
    12001 actcatggta cttatcctgg cacagttact gcaacagaag actcaatcgt agtggatggc
    12061 gaagcaactc gtgtttatgc agaacccgac gcaagcaaaa tcccttgggc aaaagaaaac
    12121 ggtgtagata tcgtactaga atgtactggt ttttatacat ctgaagaaaa agcaaaagcg
    12181 catttagatg ctggtgtcaa acgagtagtt atttctgccc cagctggtgc tatgaaaacc
    12241 atcgtctata acgtcaatga tgatacatta gatgaaaacg acaaaatcat ctcagcaggt
    12301 tcatgtacaa caaactgttt agcacctatg gcttatttct taaataatga atttgggatc
    12361 gaagttggta caatgacaac agttcatgcc tacacttcca cacaaatgct cttagatgga
    12421 cctgtaaaag gcggtaattt gcgtgctgct cgttcagctg ctgacaatac tatcccacat
    12481 tctactggtg ctgcgaaagc aatcggctta gttatccctg agctacaagg aaaattacaa
    12541 ggacacgcac aacgtgttcc agtcgttgat ggttcattaa ctgaattggt ttcagtattg
    12601 aaaacaaaag taactgctga tcaagtaaac gaagcaatca aaaaacacac agttgacaat
    12661 ccttcattcg ggtatgatga ccgtcaaatc gtttctagcg atgtcattgg aacaactcaa
    12721 ggttcgatct ttgacccaac gcaaacagaa gtaacaactg ctggtgattt ccaacttgtt
    12781 aaaactgttg catggtatga caatgagtat ggatttactt gccaaatgat tcgcttacta
    12841 gaaaaattcg ctaacttgta atttgataga tcaatcgata aaagaagcct ttgactggat
    12901 ccagtcaaag gcttctttct tttcatcccc tacttttaaa ctcgttcaat gaacatattg
    12961 gcttaaaaat tgttggacta cttgctgata tttttcagga tctgtcttgt atgcttccgc
    13021 atgctcggct ccttttacaa catacttttc ttttggtccc ttcgttgcac gatagacctt
    13081 atctaacatt tcaaatggta caaatgtatc ttcgtctcca tgtataaaga gcattgggcg
    13141 ctggtttttc ttcaattgtt ccactgcatt tgcttcaccg aaaaagtagc cagcacggac
    13201 ttttgtcact agactcgtta cttgcataag cggaaatgct ggaagactaa acatatcgtc
    13261 tagctgataa gcaagctcat catgaacgga agaataaccg caatcttcaa tgattgcttt
    13321 cacattatct ggtagttttt ctccgctagt catcataact gttgctgctc ccatgctgac
    13381 accgtataga acgatttctt gcgattttcc attgttttct agcactttgt ttatccactg
    13441 tacatagtct ttacgctctg gccatccaaa tccaatgtaa tccccttcgc tttttccatg
    13501 tcctcttgca tcaggaacaa ggacattgta acccatatca tgaaacatct ttgcgtaatt
    13561 ggtcatcgtt tcagcgtttc ccatatagcc atgagccaca agagctgttt tttcactttt
    13621 cgtttcagca ggaagataaa tcgctgataa tttcagaccg tcttttgaag tgatggtcca
    13681 atcgcttcgg ttttcttttg aactaaacca tttatgtgca tccgtttctt tggatgtctg
    13741 tgttgtacca ggtgtatctt cgtctaaaaa gtctttctca gaaggcacaa cagcataatt
    13801 ataaaaataa ttccctgcaa acactaacgc aacaatcaga gctaaagcga cgcctaaaag
    13861 gattttcatc caaagtttca taaatcatcc atctcatttc tcttttttca tataaccttg
    13921 aattagtata atcaaagaat ctctactatg caatgttcat ttttataac
//
LOCUS       DAZKLD010000074        13968 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.074, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000074 DAZKLD010000000
VERSION     DAZKLD010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13968)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13968)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13968
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.074"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(248..817)
                     /gene="thiT"
                     /locus_tag="ACG8KR_001752"
     CDS             complement(248..817)
                     /gene="thiT"
                     /locus_tag="ACG8KR_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015234 - thiamine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HFQ0201119.1"
                     /translation="MGKNRVWVEGTIVAALAMVLSLIPIQIGSSFSISLGQIPLTLFA
                     LRRGTKAGMLAGFIWGILHFPLGQVYYLSVVQVLIEYPIAYTFAGAAGLMASNVQKGY
                     VDDNTKQIRKSIVFGALIGALSRYFWHFIAGVVFWGAYALWGMNPWLFSFVMNGASGL
                     STAIVTIIVLLGIQQTTPSIFVPDKRQEL"
     regulatory      complement(883..985)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(991..1962)
                     /locus_tag="ACG8KR_001753"
     CDS             complement(991..1962)
                     /locus_tag="ACG8KR_001753"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HFQ0201120.1"
                     /translation="MSKHYFDPRLKIFALNSNRPLAEKIAAAVGVELGKSSVTQFSDG
                     EIQVNIEESIRGSHVYVIQSTSSPVNDNLMELLIMIDALKRASAKTINVVMPYYGYAR
                     QDRKARAREPITAKLVANMIQKAGATRMLTLDLHAVQIQGFFDIPVDHLMGAPLIANY
                     FLEKGIKGDDVVVVSPDHGGVTRARKLAEFLKSPIAIIDKRRPKANVAEVMNIIGQVK
                     GKTCVLIDDMIDTAGTITLAANALKEAGATSVYASCTHPVLSGPALQRIEDSAIERLV
                     VTDSIYLPEDRKIDKIDEVSVGGLMGDAIKRIHENKPVSPLFETKNK"
     gene            complement(2056..3429)
                     /gene="glmU"
                     /locus_tag="ACG8KR_001754"
     CDS             complement(2056..3429)
                     /gene="glmU"
                     /locus_tag="ACG8KR_001754"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HFQ0201121.1"
                     /translation="MDARYAIILAAGKGTRMKSKLYKVLHPVSGKPMVEHIINRVSET
                     KPDEVITIVGHGAEQVKAQLGERSKYALQAEQLGTGHAVLQAASFLEGKKGTTLVISG
                     DTPLLTTETLNNLFEYHQGKNASATILTAQAENPTGYGRIIRDHIGIVEKIVEQKDAT
                     PEEALVQEINTGTYCFDNEALFDALSKVGTNNAQGEYYLTDIIEILKEEGHTVAAYQT
                     DDFEESMGVNDRIALAKANEIMRKRINQMHMVNGVSFVDSATTYIDAGVEIGPDTLIE
                     AGVQIQGNTVIGSDCVIGSHSKIVDSRIEDHVVIENSVIESSHVKKHADVGPYAHLRP
                     KAEIGENVHIGNFVEVKNAQIGKGTKVGHLTYVGDATLGEEINVGCGVVFVNYDGKNK
                     HHTTVGDHSFIGSSTNIIGPVEVAKNSSIAAGSTITDNIPEYALAIARARQVNKEGYA
                     KKLPYLN"
     gene            complement(3932..4750)
                     /gene="purR"
                     /locus_tag="ACG8KR_001755"
     CDS             complement(3932..4750)
                     /gene="purR"
                     /locus_tag="ACG8KR_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0045982 - negative regulation of purine
                     nucleobase metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HFQ0201122.1"
                     /translation="MKVRRSERLVDMTQYLLNHPHELISLTSFAERYESAKSSISEDL
                     GIIKKTFKERGYGTLETIPGAAGGVLFIPEISYEEAKKYIESLAERLSEQDRLLPGGY
                     VYLSDLLGEPELLRQVGRIIASKYLGEKIDAVMTVATKGVPIAQAVSYYLNAPFVIVR
                     RDSKITEGSTVSVNYVSGSSERIEKMELSKRSLKRGSRVLVVDDFMKGGGTVNGMKSL
                     IEEFESELVGITVFAESKFNGHRAIDDYTSLLYVKDVDTQTKNITVVPGNYFSE"
     gene            complement(4917..5741)
                     /locus_tag="ACG8KR_001756"
     CDS             complement(4917..5741)
                     /locus_tag="ACG8KR_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HFQ0201123.1"
                     /translation="MALLSYEFMRRAFLAAIFIAGIAPMLGVFLVIRRQSLMADTLSH
                     VSLAGVALGFFLNLNPTLTTMLVVVLAAVILEYLRTIYRTYSEISIAILMAGGLALAL
                     VLMNLSGGNSATSIQSYLFGSIVTITWEQVIFLGILFVAIGILFFLFKRPMYVLTFDE
                     DTAHVDGLPVHLMSMMFNVITGVAIAVMIPIAGALLISAIMVLPAAIGMRLGKSFNVV
                     IAISVCVGFLGMLSGLTSSFYLDTPPGATITLVFIALFLIVNIIKRILVAFRRKNN"
     gene            complement(5714..6394)
                     /locus_tag="ACG8KR_001757"
     CDS             complement(5714..6394)
                     /locus_tag="ACG8KR_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HFQ0201124.1"
                     /translation="MRYIEISDLTFYYDDEPVLEDVSYHVDAGEFVILTGENGAAKST
                     LVKASLGLLKPSKGMIKIAEKNQEGKKLSIGYIPQQVASFNAGFPSTVIELVRSGRYP
                     RGRWFKRLSEKDHQHVQRALEAVGMWDMRNRRIGELSGGQKQRISLARIFATDPDLFV
                     LDEPTTGMDEESRNEFYRLLRHSAHVHGKSVLMITHDHEDIKQYADRQIRLVRKEDSQ
                     WRCFHMNS"
     gene            complement(6417..7355)
                     /locus_tag="ACG8KR_001758"
     CDS             complement(6417..7355)
                     /locus_tag="ACG8KR_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HFQ0201125.1"
                     /translation="MKKYLLTATVMIGALLFAACGNTNKEADKKEDLTIVTTFYPMYD
                     FTKEIVGDEGNVKLLIPAGTEPHDFEPSAKERAEISDADVFVYNSSDMEFFVDSLKDS
                     VDSKQTLMIEAAKGIDRLESQEADEHEESEEGHGHSHEYDPHVWLDPVLAIKEVRTIA
                     EELGEKYPDKKETFTKNADAYIKKLEALDQKYSDELKDATNRTFVTQHAAFAYLANQY
                     DLKQVAISGVSPDQEPTPSRLAELKEFVKKNNIKVIYFEENASSKVAETLSNETGVKL
                     EVLNPLESLTNEQIKAGENYISVMEKNLKALKESIN"
     gene            complement(7600..8451)
                     /gene="ispE"
                     /locus_tag="ACG8KR_001759"
     CDS             complement(7600..8451)
                     /gene="ispE"
                     /locus_tag="ACG8KR_001759"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HFQ0201126.1"
                     /translation="MELIEKAPAKINLGLDVLYRRQDGYHELEMVMSSVDLADHLFFE
                     EITEDKIIVETNKAFLPVDERNNVYQAALLLKNKYNIKTGVKIFVKKNIPVAAGLGGG
                     STDCAAALRGINQLWNLGCSLEELAEIGLEVGTDVPYCVYGSTAFVGGKGEEIQVLPS
                     MPQCWVVLVKPRMSVSTRTVFREVDTQAIQHQDIHGLAEAIREQDYQQMIKKMGNSLE
                     DITGKRHPVIHQIKERMIKYGADAALMSGSGPTVFALCQHYSRAQRVYNGLKGFCEEV
                     YLVRTLQ"
     gene            complement(8605..11178)
                     /gene="mprF"
                     /locus_tag="ACG8KR_001760"
     CDS             complement(8605..11178)
                     /gene="mprF"
                     /locus_tag="ACG8KR_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HFQ0201127.1"
                     /translation="MKNKRYLMLQWIKEHSLILKLIFFGSVLVFVANQVANIANGMSW
                     QDIWQRMGQQSRMTILLMILAGLLGVTPMLLYDWVTIRVLEKQGKPKMERKEFLLAAW
                     VTNTINNLAGFGGVVGASLRASFYGKQTNRKMVLATVSKVALFMLTGLSVWSFLTFID
                     VFFIQSESIFRSYWIWLFGGSFLAPVIFFFAYLKRKTLFFEFYPKGLLGLLLASFGQW
                     TGALAVFLIIGKLMRIDISMLSIYPMFMIATLIGMLTMVPGGMGTFDVLMILGMSQLP
                     VRQDVAVVWLLYYRLFYYVLPFMTGLLLFITHTGVKINRFLDNIPRLFFQKVAHIVLV
                     SAVYFAGIMMVLLSTITNLSAISRLFQVLLPFSFNFLDQTVNLLVGFLLLGLARALSV
                     KVKKAFLPTIALLSFGILNTITRTLSWQLILVYVLILLAVWLSRKEFYRTKFVYSWGA
                     IIFDASLFGFLFIVYAIAGYHSGRFLVGRITGNHFILFPSDDVWFSGLIGLGISLLAL
                     VALYQYLAEDTTKLGSEYEKDRFDRLLETYGGTATSHQLRLSGYSYFYYQEDDQDQVV
                     FGYQIKGNKCFVLGDPVGNPKMMRAATNQFMKQADCLGYQLAFYKISESYVVMLHELG
                     FHFTKVGEAGIVDLATEDLSRLSQQLEYQRLMKEGYHFTWYDQLPEELHQAVNEVSQK
                     WLQGAREKYFSVGRFDKSYVLSSGVGIVRKDQKVVGFITAQPMTKKQAGYDLLRVLPK
                     ESEELADYLLANLFIVYEKMGYSEANLGLAPLANVGETDFSFFQEKVMHIVYNYGNFF
                     YSFQSAYEKKLKYVDHWEGRYFAYRKGSNFGFATMQLLSLIGKEKGNVPSLAEEVLTE
                     L"
     gene            11319..12125
                     /gene="proC"
                     /locus_tag="ACG8KR_001761"
     CDS             11319..12125
                     /gene="proC"
                     /locus_tag="ACG8KR_001761"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HFQ0201128.1"
                     /translation="MKVGFIGTGNMAQAIIDGLLKKGTVSKEEVFVSSGHFENAQKFA
                     AETGVHACKSNQEVASQSDIVLLAVKPKIIEKVLEELREYSQKVLFISIASGKSLTDL
                     ETALEDPQASIVRVMPNVNVSVGAGVSAICKNEAATEEQLQQATMIFEAIGSVYPLAE
                     SDFSTFIGLAGSSPAFVYMLIDAMGRTGVLNGLSKQTATEIAAKAIMGSCQKFLSSDK
                     NPWELVDQVSSPGGTTVAGVVALEEAGFIPAIIKGINTTIEKDLEMLNKK"
     gene            complement(12204..13760)
                     /gene="rny"
                     /locus_tag="ACG8KR_001762"
     CDS             complement(12204..13760)
                     /gene="rny"
                     /locus_tag="ACG8KR_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HFQ0201129.1"
                     /translation="MVFNILLAIIGLIVGLGLGFMVAKSRHEKEIAGAQSSAAGIIDS
                     AKKEAETLKKEALLEAKEENQKYRSEVESELRESKLELKSQENRLIQREQTLNRKDDS
                     LEKRENSLESKEEALSSKQQLIEEREKDVEKMIEDQQKELEKIAGLSKEDARDVIMKS
                     TEEELNHELTLMVKESEQRAKEEADRKAKNLLSLAIQRCAADQVSETTVSVVTLPNDE
                     MKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVVLSGFDPIRREIARMTLEKLIQDGR
                     IHPARIEEMVEKSRKEMDERIREYGEEAAFEVGAHTLHPDLIKIIGRLRFRTSYGQNV
                     LKHSVEVAKLAGILAAELGEDIQLAKRAGLLHDIGKALDHEIEGSHVEIGAELAAKYK
                     ENPVVINAIASHHGDVEATSVISVLVAAADALSAARPGARSESLENYIRRLRSLENIS
                     NSFAGVESSYAVQAGREVRVMVKPEEISDLDAVRLVRDIRKRIEEELDYPGHIKVIVI
                     RETRATDYAK"
BASE COUNT         4043 a         3083 c         2417 g         4425 t
ORIGIN      
        1 atggtaaata tcgtatccca atctatgtga ttgaagatta tatttggaaa ttaaaaggca
       61 cccggtatcg agaagaggaa atcatcgcta ctttaatcta ctctgtcacc gatcgacact
      121 ggcaattggt agaagatgcc gatctttctt atctgccatt gaagtagatc tatttcttat
      181 caaaaaataa aaaggaaaca agaaactagg acatgattct gtctcaagtt tcttgcttct
      241 tttttattta cagctcctgt cgtttatctg gcacaaaaat cgaaggagtc gtttgttgga
      301 tccctaataa aacgatgatc gttacaatag ctgtactcaa cccgctggca ccattcatta
      361 cgaaagaaaa gagccaagga ttcatccccc atagtgcata cgctccccag aaaaccactc
      421 ctgcaataaa atgccaaaaa tatctggaaa gtgcgccaat caatgcacca aacacgatgc
      481 tttttctaat ttgtttcgta ttatcgtcta catagccttt ttggacattt gaagccatga
      541 gccctgctgc gcctgcaaaa gtataggcaa tcgggtactc aatcaacact tgaacgacac
      601 ttaaataata aacttgtccc aacggaaaat gtaggatgcc ccaaatgaaa cctgccagca
      661 tccctgcctt tgtccctctt cttaacgcaa aaagagtcaa tggaatctgc cctaatgaaa
      721 tagaaaaact gctgccgatc tggatcggaa tcaacgacaa caccatcgct aacgctgcaa
      781 caatcgttcc ttctacccaa actctatttt ttcccataaa aaaacttcct cctttgaata
      841 aaaaagtcac aaaggaggaa gcagctttcg aaaatagtca aacagctgtt tattcacaat
      901 tcctacgctc gcataaccga tacaggtacg aagggtttag aaatagattc aatctcagcc
      961 caattggact cccctttgtg agtttattta ttatttattt tttgtttcaa acaatggact
     1021 aactggtttg ttttcatgga tacgtttgat tgcatctccc attagtccgc caacacttac
     1081 ttcatcgatt ttgtcgattt tacgatcttc tggaagatag atcgagtctg taacaactag
     1141 acgctcgata gctgaatctt ctattctttg taaagcaggg ccggataata ctggatgtgt
     1201 acatgaagca tacacactag ttgcaccagc ttctttcaaa gcattggcag ccaatgtgat
     1261 cgtacctgct gtatcgatca tatcatcgat cagtacacaa gttttgcctt tgacttgtcc
     1321 aatgatattc attacttctg caacatttgc ttttggacgg cgtttgtcta tgatcgcaat
     1381 cggagatttc aaaaattccg ctaatttacg tgcccgtgtg acaccaccat ggtctggtga
     1441 aacaacgaca acatcatcgc ctttgattcc tttttctaag aaataattgg caataagtgg
     1501 cgcacccatc aaatgatcga ctggaatatc aaagaaacct tggatttgaa ctgcatgaag
     1561 gtccaaagtc agcattcttg ttgcaccagc tttttgaatc atgtttgcta ctaatttcgc
     1621 tgtgattggt tcccgtgcac gtgctttacg gtcttgacgc gcatagccat aataaggcat
     1681 gactacgttg attgtttttg cacttgctcg tttcagtgcg tcaatcatga tcagcaactc
     1741 catcaagtta tcattgactg ggctgctcgt tgattgaata acatagacat gcgatccacg
     1801 aatactttct tcaatgttta cttgaatttc tccatcacta aattgagtaa cagatgattt
     1861 tcctaactcg acgccgactg ctgctgcaat tttctctgct aatgggcgat tggaatttaa
     1921 cgcaaaaatt tttaatcttg ggtcaaagta atgttttgac atggggacct ccactttctt
     1981 tttcacaact ctagaattct acctaataaa tagtagtcat attttttaaa taaatcaagt
     2041 gattttgcaa aagtcttagt ttagataagg gagttttttt gcatatcctt ctttattgac
     2101 ttgtcttgct ctcgcaattg ccagcgcata ttctggaata ttgtctgtaa ttgttgaccc
     2161 ggcagcaatc gaactgtttt tagctacctc gacaggtccg atgatattcg tgctagaacc
     2221 aataaagctg tggtcgccga cagtcgtatg atgtttgttc tttccatcat agttgacgaa
     2281 gacaacacca catccgacat tgatttcttc tcccagcgta gcatcaccaa cataagtcaa
     2341 gtgaccgact ttagttcctt tgccgatttg agcatttttc acttccacaa agtttccgat
     2401 atggacgttt tcgccaattt cagcttttgg tcgtaaatgt gcataaggtc ccacatctgc
     2461 atgtttcttg acatggctgc tttcaatgac tgagttttca atgacgacgt gatcctcaat
     2521 cctactgtct acgatttttg aatgtgaccc gatgacacaa tcagaaccaa tgactgtatt
     2581 tccttggatt tgtacacctg cttcgatcaa tgtgtctgga ccgatttcta cgcctgcatc
     2641 gatgtatgtc gttgctgaat cgacaaagct tacgccattg accatatgca tctggttaat
     2701 tcgctttctc atgatctcat ttgctttagc taacgcgatc cgatcattga ctcccattga
     2761 ttcttcaaaa tcatccgttt gataagcagc aactgtatgg ccttcttctt ttaagatttc
     2821 gatgatatct gttagatagt attcaccttg agcattgttt gttccaactt tgcttaacgc
     2881 atcgaacaaa gcttcgttgt caaaacagta agttcctgta ttgatttctt gtacaagagc
     2941 ttcttctggt gttgcatctt tttgttccac gattttttct acgatcccaa tatggtcacg
     3001 gatgattcgt ccatatcctg ttgggttttc tgcttgtgcc gtcaagatcg ttgcacttgc
     3061 attttttcct tggtgatatt caaaaagatt gttcagcgtt tcagtagtca ataatggtgt
     3121 atcgccactg ataactaacg ttgttccttt ttttccttcc aagaaagaag cagcttgcaa
     3181 aacagcatgt cctgttccca attgttctgc ttgtaacgca tatttactgc gttctcctag
     3241 ttgtgcttta acttgttctg ctccgtgtcc aacgatcgta atcacttcat ctggtttcgt
     3301 ttcactgacc cgattaatga tatgttctac cataggttta ccagaaacgg gatgtaaaac
     3361 tttatataat tttgacttca tgcgggtacc tttccctgcc gccaaaataa ttgcataacg
     3421 tgcgtccaac tgcgtcactc ctaaatatag tttgctgatt tcagtttatc ccagtatacc
     3481 cattttttca acaaaacaag tgattttctt ttatcccagc gttttctgga taaaaaaata
     3541 gatagaccat aacgggccta tctatttggt aaccggtgct ctcctgccaa ctcctcggtc
     3601 acaagtcaca ctactcataa aacatgaaaa gctgtttttt gaaatcgttt cttgtgactt
     3661 agagttaaac ggtaggctta caacctcttc attaaacgtt tttcaatcag ctgaccttcg
     3721 gtattagctc aataaacggc aaaatatgag gagcaatttt tccgtttatt gagctaatac
     3781 tcaggacagg acgctgattt cacaacctct agataaacgg tgttcaaaag ccagtttctc
     3841 ggtattaagc caaatttatg aaaaaatgtg gaaagccatt ttcacaaaat ttttcttact
     3901 actcgaaact aagcagcttt ttcacaacct cttattctga aaaataattt cctggcacaa
     3961 ccgtaatatt tttcgtttga gtatctacat ctttgacata aagcagtgac gtataatcat
     4021 cgatggcgcg atgaccgttg aattttgatt cggcaaatac agtgatgcca acaagttctg
     4081 attcgaattc ttcaatcaag ctcttcattc cgttgactgt tccgccgcct ttcatgaaat
     4141 catccactac aaggacacgt gaaccacgtt tcaaacttct cttagacagt tccatctttt
     4201 caattctttc agaagatcca gacacatagt tgacacttac tgttgatcct tctgttattt
     4261 ttgagtcccg gcgtacaata acgaatggcg cattcagata ataggacaca gcttgggcaa
     4321 taggtacgcc ttttgtggct acagtcatta cagcatcgat tttttcacca aggtatttag
     4381 aagcaatgat tcttccaact tgacgcagca gttcaggttc tcctaacaaa tcagagagat
     4441 aaacatatcc tcctggaagc agacggtctt gttctgatag acgttctgct agagattcaa
     4501 tatatttttt cgcctcttca tatgaaattt cagggatgaa caatacacca cctgcagcac
     4561 ccgggattgt ttccaaggtt ccataccctc gttctttaaa tgttttcttg atgattccca
     4621 aatcttcact gatagaagat ttagcagatt cataacgttc ggcaaagctt gttaaggaaa
     4681 tcaattcatg cgggtgattc aataaatact gtgtcatgtc aactagacgt tcactgcgac
     4741 gtactttcac ctattccaac tccttttaag aaattttatc gttttacatt atataagttt
     4801 ttcttcaaaa attcgagcat tttatgaaaa aataagaaaa tagttcggta tttattaaaa
     4861 taaaaagaag cctataaaaa atttttcgtt tttataaggc ttcttttctt tataatttag
     4921 ttattttttc ttctgaaggc aaccaatatt cgtttgatga tatttacaat caggaacaaa
     4981 gcaataaaga ctaatgtgat tgttgctcct ggaggtgtat ctagataaaa cgagctagtt
     5041 agtcctgaca gcatccctag aaatcctaca caaacactga tagcaatcac gacattgaag
     5101 cttttgccta atcgcatacc gattgctgcg ggaagaacca tgattgcgga tatcaaaagt
     5161 gcaccggcaa tcgggatcat cacagcaata gccacaccag taatcacatt gaacatcatc
     5221 gacatcaaat gaacgggtag cccatccacg tgggcggtat cttcatcaaa agtcagaaca
     5281 tacatcggcc gtttgaataa aaagaacaat ataccgatgg caacgaataa gattcctaaa
     5341 aagatgactt gctcccatgt aatggtcacg atcgaaccaa acagatagga ttggatactt
     5401 gttgctgaat tccctccgct caaattcatc aatactaacg ctagcgccaa accaccagcc
     5461 attaaaatgg caatggagat ttctgaatag gttcggtaga tcgttcgcag atactccaat
     5521 atcacagctg ctaatacaac gactagcatg gtcgtaagtg taggattaag gttcaaaaag
     5581 aatcctaacg ctacccctgc taaagagaca tgagaaagcg tatcagccat cagcgattgt
     5641 cttctgatca ctaaaaacac acctagcatc ggggcgattc cggcaatgaa gatagcggca
     5701 agaaacgctc ttctcatgaa ttcatatgaa agcaacgcca ttgagaatcc tcctttctca
     5761 ccagtcggat ctgtctgtcc gcatactgtt tgatatcttc atgatcatgg gtgatcatta
     5821 gaactgattt cccatggaca tgcgcactat gtcggagaag acgataaaat tcatttctgg
     5881 attcttcatc catacctgtt gttggttcat caagaacaaa taagtcaggg tcagtagcaa
     5941 aaatacgcgc taaactgatt cgctgctttt gtccgccaga caattcacct attcgccgat
     6001 ttcgcatatc ccacatccct acagcttcta aagcacgctg tacatgttga tgatcttttt
     6061 cagagagacg cttgaaccag cggcctcttg gatatcttcc tgaacggact aattcgatca
     6121 ccgtactagg aaaacctgca ttgaaagatg caacttgttg aggaatataa ccgatactta
     6181 atttttttcc ttcttgattt ttctcagcaa tctttatcat tccttttgaa ggtttcagta
     6241 atcctaacga tgctttcacc aaggttgact tggctgctcc attttctcca gttaaaatga
     6301 caaattcacc cgcatctaca tgataagaga catcttctag tacaggttca tcatcatagt
     6361 aaaaggtcaa atcagatatt tcgatataac gcaccttaca acctccattc taaaacttaa
     6421 ttgatacttt cttttaaagc tttcagattt ttttccatga cagaaatata attttcgcct
     6481 gctttgattt gttcattagt caagctttct aaaggattca acacttctag cttcactcct
     6541 gtttcattcg acaatgtctc agctaccttc gaagaggcat tttcttcaaa ataaatcact
     6601 ttgatgttgt ttttcttcac aaattctttt agctcagcta agcgacttgg tgtgggttcc
     6661 tgatctggtg aaacgcccga aatcgctact tgttttaagt cgtattgatt cgctaaataa
     6721 gcaaaagcag catgctgagt gacaaatgtg cggtttgttg catcttttag ctcatcggag
     6781 tatttttgat ccagtgcttc aagctttttg atataagcat cggcattctt cgtaaaagtt
     6841 tcctttttat ctggatattt ttcgcctaat tcttcagcaa tcgttctgac ttcttttata
     6901 gctaataccg gatcgagcca aacatgtgga tcgtattcat gtgaatgacc atgcccttct
     6961 tctgattctt catgttcgtc tgcttcttgc gattctaaac ggtcgattcc tttagctgct
     7021 tcgatcatca aggtctgttt gctatccact gaatccttta acgaatccac aaaaaattcc
     7081 atgtctgaac tattatacac gaaaacatct gcatcggaaa tttctgctct ttcttttgca
     7141 cttggttcga aatcatgcgg ctccgttcca gcagggatca aaagtttcac gtttccttca
     7201 tcccctacga tctcttttgt aaaatcatac atcggataaa aagtcgtaac gatcgtcaaa
     7261 tcctcttttt tgtctgcttc cttgttcgta tttccacatg ctgcaaacag taaagcacct
     7321 atcattaccg tagctgtcaa aagatatttt ttcatttata tgctcctttc aatgaacaaa
     7381 tcggaatttt acgatttatt aacgtgaact aatgtatcaa aataaaaaaa cacagtcaat
     7441 agaaatccat caatttctga tttaagcatg tatattcttg attaaatcgt aaaatggatg
     7501 agttgctgct catatctagc catttcacta caataaaaaa ctgaagaata ccattttagg
     7561 attcctcagt ttttcatcac ctattttact tcttatattt tattgcaggg ttcgtactaa
     7621 ataaacttcc tcacaaaaac cttttaaccc attatatact ctctgcgctc ttgaataatg
     7681 ttgacacaag gcaaaaacgg ttggtccgct tccactcatc aatgcagcat ctgctccgta
     7741 cttgatcata cgctctttga tttgatggat gactggatgc cgctttcccg ttatatcttc
     7801 taaactgttt cccatttttt tgatcatctg ctgataatct tgctctcgga tggcttcagc
     7861 aagtccgtgg atgtcttgat gttggatggc ttgcgtatct acttctctaa acactgtacg
     7921 tgtagagaca ctcattcttg gtttgaccaa cacaacccag cactgtggca tagaaggtaa
     7981 tacttggatt tcttcccctt tgccaccgac aaatgccgta cttccgtaga cacagtaagg
     8041 aacatctgta ccgacttcca aaccgatttc tgccagttct tctaacgaac agcccagatt
     8101 ccataattgg ttgatccctc ttagagcagc tgcacagtct gtacttcctc cgcctaatcc
     8161 agctgctact ggaatattct tcttaacaaa gatcttgaca ccggttttga tattgtattt
     8221 attttttagc aataaagcag cttgataaac attgttccgt tcatctaccg gtaaaaatgc
     8281 tttatttgtt tctacaatga ttttgtcttc tgttatttct tcaaaaaata gatgatctgc
     8341 caagtctacg ctcgacatca ccatttccaa ctcatgatac ccatcttgcc gtctatataa
     8401 tacatccagt cctaaattta tcttggctgg cgctttttca atcaattcca tcctctcacc
     8461 cattcgaaaa gtctaagtcg caagtattct atcataggga aaaataaaag tatagcttat
     8521 gtctgcctag tggttgattt tgtcatcgtt tcaacagctt tcacagaatt ttctcttcat
     8581 aatccaaaca agttctagtg attcttataa ttctgtgagc acttcttctg ccaaactcgg
     8641 aacatttcct ttttcttttc ctatcagact gagtagctgc atggtagcaa atccgaaatt
     8701 acttcctttc ctataagcaa aataacgtcc ttcccaatga tcgacatatt ttaatttctt
     8761 ttcataagca gactggaagg aatagaaaaa attaccataa ttatatacga tatgcatgac
     8821 tttttcttga aagaaagaaa aatctgtttc tccaacatta gctaatggag ctaagcctag
     8881 attggcttcc gagtagccca ttttctcgta tacaataaac agattggcta acagatagtc
     8941 agctaattcc tcagattctt taggtaagac ccgcaacaaa tcatatcccg cctgcttctt
     9001 cgtcatcggc tgagcggtga tgaagccaac gactttttga tctttacgga caataccgac
     9061 accgctagat aatacatatg atttatcaaa tcgtcccaca gagaagtatt tttctcgtgc
     9121 accttgaagc catttttgtg aaacttcatt tacagcttga tgtaattctt ccggcagttg
     9181 atcgtaccaa gtaaaatggt atccttcttt catcaaacgt tgatattcaa gttgctgact
     9241 cagccgagac aagtcttctg ttgctagatc aacaatccct gcttcaccta ctttcgtgaa
     9301 gtgaaaaccc aattcatgaa gcatcactac gtagctttct gatatcttat aaaacgcaag
     9361 ctgatagcct aaacaatctg cttgtttcat aaactgattc gttgctgccc gcatcatttt
     9421 aggattgcca actggatctc ctaaaacaaa gcatttgttt cctttgatct gataaccaaa
     9481 aacgacttga tcttggtcgt cttcttggta ataaaaataa ctgtaacctg acaagcgaag
     9541 ctgatggctt gtagctgtgc ctccgtatgt ttccaataaa cggtcaaaac ggtctttttc
     9601 gtactcactg ccaagcttcg tagtatcttc agctaaatat tgataaagcg ccactaaagc
     9661 tagcaatgag atgcccaaac caataagccc tgagaaccac acatcatctg aaggaaacaa
     9721 aataaaatga tttcctgtta ttcgaccgac taaaaagcgt ccgctgtgat agcctgcaat
     9781 tgcgtaaacg ataaacaaaa agccgaacaa gctagcatca aagattatag ctccccaaga
     9841 ataaacgaat tttgttcgat aaaattcttt tctggataac cacacagcta acagaatcaa
     9901 gacatatacc aatatcaact gccaagataa cgtacgtgtg attgtattta aaataccaaa
     9961 agatagtaaa gcaatcgtag gcagaaatgc ctttttgact tttactgaaa gtgctctagc
    10021 aaggccgagt aataaaaagc caaccaatag attcactgtc tgatctaaaa aattgaatga
    10081 gaacggcaat aatacttgaa atagccgact gattgcagac agattagtga ttgttgatag
    10141 aagtaccatc atgatccccg caaaatagac agcacttacg agcacgatat gcgcaacctt
    10201 ttggaaaaac agtcgcggaa tgttatctaa aaatcgattg attttgactc ctgtatgtgt
    10261 gatgaacaat agaagtcccg tcataaaagg tagaacataa taaaacaacc gataatataa
    10321 tagccaaact actgctacat cttgccgaac aggcaattga ctcattccta agatcatcaa
    10381 aacgtcaaaa gtccccattc cacctgggac catcgtcagc attcctatca atgtagcaat
    10441 catgaacata ggatagatcg aaagcatcga aatatctatt ctcatcaact tgccgatgat
    10501 caaaaatacc gcaagggctc ccgtccattg tccaaaggat gccagtagca gaccaagcaa
    10561 gccttttgga taaaactcga agaacaatgt ctttctcttt agataagcaa aaaagaaaat
    10621 gactggtgct aaaaaactac cgccgaaaag ccagatccaa tagcttcgaa aaatactttc
    10681 tgactgaata aaaaagacat caataaatgt aagaaacgac catacagaca aaccagtcag
    10741 cataaatagt gcgacttttg atacagtggc taaaaccatt tttctatttg tctgctttcc
    10801 ataaaaacta gcccgcagac ttgctcctac cacaccacca aaaccggcga gattgttgat
    10861 cgtgtttgtt acccacgcag caagaagaaa ctcttttctt tccatttttg gttttccttg
    10921 tttttctaag actctgatcg tcacccaatc atacaacaac atcggagtga cacccagcag
    10981 tccagccaga atcatgagca ggatcgtcat gcggctttgt tgtcccatcc tttgccaaat
    11041 atcttgccaa ctcatgccat tagcaatatt tgcgacttga tttgccacaa agactaacac
    11101 ggagccgaaa aaaatcagtt ttaaaatcag actatgttcc tttatccatt gcagcatcaa
    11161 gtatcgttta tttttcacat tttcccattc ctaacttgta tacttttgtg tcatacccca
    11221 atgttatctt atttatttta atttttccac tttagacctg ttctttgctt gcgatgatag
    11281 aaaaagaaag atatcctaat gctaaaggag aggataacat gaaagtagga tttatcggca
    11341 cagggaatat ggcacaagct attattgatg gtttattaaa aaaaggaaca gtctcgaaag
    11401 aagaggtgtt cgtttccagt ggacattttg aaaatgcaca aaaatttgct gctgaaactg
    11461 gcgtccatgc atgcaaaagt aatcaggaag ttgcatcaca atcagatatc gttcttttag
    11521 ccgtgaaacc aaaaatcatt gaaaaagtat tggaagaact aagagaatat agccagaaag
    11581 tcttattcat ttctattgct tcaggaaaat cattaacaga tttagaaaca gcgcttgaag
    11641 atcctcaggc ctccatcgta cgtgtgatgc cgaatgtcaa tgtcagcgta ggagcaggcg
    11701 tttctgcgat ttgcaaaaat gaagctgcca cggaggaaca gcttcagcaa gctacgatga
    11761 tttttgaagc aatcggcagc gtctatcctt tagcagaatc agatttcagt acattcattg
    11821 gccttgctgg cagctcaccc gcattcgttt acatgctgat cgatgcgatg ggacggactg
    11881 gtgtactgaa tggtttgtcc aaacagacag ccactgagat tgctgcaaaa gcaattatgg
    11941 gttcttgtca aaaattcctt tcaagtgata aaaatccatg ggaacttgta gaccaagttt
    12001 cttctcctgg cggtacaact gttgcaggtg tagttgcctt agaagaagca ggattcatcc
    12061 cagcaatcat caaaggaatc aatacgacga tcgagaaaga tcttgagatg ttgaataaaa
    12121 aataatgata gcaatgtttt ttcgtgcctt acaaaaaaag aagagctgtg gcttttgccg
    12181 cggctcttct tttgtatttt ttattattta gcataatcag ttgcacgagt ttcccgaata
    12241 acgattactt tgatgtgtcc aggataatcc agttcttctt cgattcgttt acgaatatca
    12301 cgtactaaac gaacagcgtc taaatccgag atttcttctg gcttgaccat tacccgaact
    12361 tcgcgtccag cttgaactgc gtaacttgat tctacaccgg cgaagctgtt cgaaatattt
    12421 tccagacttc ttaaacgacg gatgtagttt tctaatgatt cacttcgcgc accaggtctt
    12481 gctgctgaga gcgcatctgc tgcagctaca aggacagaga taacagaagt agcttccaca
    12541 tctccatgat gagaagcgat cgcgttgatt actactgggt tttctttgta cttggcagct
    12601 aattccgcac cgatctcaac gtgagatcct tcaatctcat ggtcaagtgc tttcccaata
    12661 tcatggagca acccggcacg ttttgctaat tggatatctt cgccaagctc tgctgctaag
    12721 ataccagcta attttgcaac ctcgaccgaa tgcttcaaga cgttttgtcc ataactagtt
    12781 ctgaatcgca gacgtccgat gatcttgatt agatctggat gaagtgtatg agcaccgact
    12841 tcaaacgctg cctcttcccc gtattcgcga atacgttcat ccatttcttt acgagatttc
    12901 tcgaccattt cttcgatacg cgctggatgg attcgtccat cttgtatcag tttttccaag
    12961 gtcatccgtg cgatttctct tcggattgga tcgaatccgg aaagaacaac tgcttccggt
    13021 gtatcatcga tgattaaatc gataccagtc agcgtctcaa gcgttcgaat attacgacct
    13081 tctcgtccaa tgatacgacc tttcatttca tcattcggaa gggtaacgac tgaaacagtc
    13141 gtttctgaaa cttgatctgc tgcgcagcgt tgaatcgcta atgataataa gtttttcgct
    13201 ttgcgatctg cttcttcttt ggcacgctgt tcagattctt ttaccattag agttaactca
    13261 tgattcagct cttcttcagt cgatttcatg atgacatctc gtgcgtcctc ttttgataaa
    13321 ccagcaattt tttctagttc tttttgttga tcttcaatca ttttttcaac gtctttttct
    13381 cgctcttcaa ttaattgctg ttttgaacta agtgcttctt ctttactttc aagtgagttc
    13441 tcgcgttttt ctaacgagtc atctttacga ttcagcgttt gttcgcgttg aatcaaacga
    13501 ttctcttgag attttaattc taacttactt tctctcagct cactttcaac ttcagaacgg
    13561 tatttctgat tttcttcctt agcttctaac aatgcttctt tttttagagt ttctgcttct
    13621 ttttttgcac tatcgatgat tccagcagct gatgattgtg caccagctat ttctttctcg
    13681 tgacgtgact ttgcgaccat aaaccctaaa ccaagaccga caattaaacc gatgatagcg
    13741 aggagaatgt tgaataccat aaaatccacc tccatactat ctttttcatt tttatcatct
    13801 atacagcttc tatgataaat tattctaaac tcgtcaatat gcatcttcgc atatatgtgt
    13861 acaaaaaaca tgcacaactt tattctaatg tttgtacccc gtagtgtcaa ctttaaataa
    13921 acaaagaaac ggctttctac ttattcatta aacgatggtt tttaaaat
//
LOCUS       DAZKLD010000075        13847 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.075, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000075 DAZKLD010000000
VERSION     DAZKLD010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13847)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13847)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13847
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.075"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(169..1005)
                     /locus_tag="ACG8KR_001763"
     CDS             complement(169..1005)
                     /locus_tag="ACG8KR_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HFQ0201130.1"
                     /translation="MLRFLILSGYFELMMYLQVSGKLDQYINVHYRYLAILSMILSAL
                     LALVQLYLWVKNGKAEEKAHEHDHDHGLSKPYQRGIAYVLLALPVIVGTLFPTVSLDT
                     TIVEAKGFNFPVSKESVGDPEMQTQYLKPDTSIYFNKSDYLDQMNELKEKYGDKQTIK
                     ITDENYLEVMELIYNYPSEFIGKKISYEGFVYQTPNDSNTDIFLFRFGIIHCVADSGV
                     FGLLTHMPEGVTVKNDEWYKIEGTIQSDYYQPFKREIPSVVVTNAEKVSAPKNQYVYR
                     SF"
     gene            complement(1016..1945)
                     /locus_tag="ACG8KR_001764"
     CDS             complement(1016..1945)
                     /locus_tag="ACG8KR_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HFQ0201131.1"
                     /translation="MFSFIPNSVLQMSTIFLSIVIEALPFVLLGCLISGALQVFLTPE
                     RVKRWLPENRIFSIFTGSILGFFFPSCECGIVPIVHQFVKKGVPVHTAFAFMLTAPII
                     NPIVIFSTFIAFGNSWQMASWRVIGSFFVALVIGVWLAYFQKDAVLKEKITSKVAENH
                     HHHVHEEEQSEKNEPKNQLIHQTGHVLTHSIDEFFDTGRYLIIGGLIAAAMQTYLPTR
                     IMLTLGSTKILAIIIMLLLAFTMSLCSEADAFIGSSLLSLFGTAPVAAFLVFGPMVDI
                     KNLLMMKRYFTGRFILSLIGLIALSVIGFTLFI"
     gene            2137..2490
                     /locus_tag="ACG8KR_001765"
     CDS             2137..2490
                     /locus_tag="ACG8KR_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HFQ0201132.1"
                     /translation="MKQTTNLTEVQDILQQSSVAVIYLSMPNCSVCHAVRPRLEELLT
                     HYDIPALHLDAFETPEVASKFEVLTAPVVLIFHQGKEVFRQARFIDFKKIRYLLDELT
                     AEDDSLSYEEIFRTK"
     gene            complement(2564..3220)
                     /locus_tag="ACG8KR_001766"
     CDS             complement(2564..3220)
                     /locus_tag="ACG8KR_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0051775 - response to redox state [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HFQ0201133.1"
                     /translation="MKDHIIPKATAKRLPLYYRYLRILNNAGKKKVSSTELSEAVQVD
                     SATIRRDFSYFGELGKRGYGYDVEDLMHFFAKTLNDDQLTNVALIGVGNLGSALLKYK
                     FHQSNSIRISCAFDVNEEIVGRIVDGIPVYPMEDMSEQIKIQQIEVAILTIPAERAQE
                     VVNQLTEAGIKGILNFTAARLNAPSDVLIQNVDLTNELQTLIYFLHSDANAHSENTAE
                     "
     gene            3446..5380
                     /locus_tag="ACG8KR_001767"
     CDS             3446..5380
                     /locus_tag="ACG8KR_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFQ0201134.1"
                     /translation="MILLQANQIARHFGAEVLFENIHLEISTGARVALVGRNGAGKST
                     LLKIIAGIEAPDEGKIAKNKTATLGYLAQDTGLDSNETVWNEMLKAFEQVRQMEERMR
                     ELEIFISEGTPDTPAYSSYLKEYDRLQHDFSDRNGYGYENEIRSVLHGFKFDSSFYDQ
                     KIDTLSGGQRTRLALARMLLQHPDILILDEPTNHLDIETLSWLENYLQSYTGALLIVS
                     HDRYFLDKVVNEVYELSRRKMRHYKGNYSKYLDLKAEQLASEWKAYEKQQTEIHKLED
                     FVARNLVRASTTKRAQSRRKTLEKMDRLDRPQGDEKQANFLFSIEKPSGNVVLQIEDA
                     AIGYDNVTLSEPINLDIRRQEAIALVGPNGIGKSTLLKSIIHQIPFIRGKETLGTNVT
                     IGYYDQGQADLHTNKTILAELWDEHPTTPEKEIRSALGAFLFSGEDVEKPIPLLSGGE
                     KARVALAKLAMNKENFLILDEPTNHLDIDNKEVLENALIDYDGTLLFVSHDRYFINRI
                     ATKVVELSENGSKVYLGDYDYYLEKKQEEEEIAELLAQDEPESAPAPTNKSDYYQSKE
                     QQKLIRSLQRKITQVEEEMARIDEWIDQLEQEMVAPENLEDHVKLNELNQDLEAARQD
                     QETKLTEWEELSLELEEYEN"
     gene            complement(5466..6689)
                     /locus_tag="ACG8KR_001768"
     CDS             complement(5466..6689)
                     /locus_tag="ACG8KR_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFQ0201135.1"
                     /translation="MLKAGGEMLDKVMFEEIIHPEKWINECYKELMEKEWNNEIERCR
                     RHTERLIKRISNSQYQFPSECPIKGRYPLRDNIGWTEGFWTGINWLLFEKTGDPLFQQ
                     TAEKADIDFIHRIDCQIITNHHDLGFLYSLSTVADYKITGNRKMKEASIDAAETLLQR
                     FWEKAGVIQAWGDPSDPSQQGRMIMDCCMNLPLLYWASEETGNQKYKKAAQAHIQNAA
                     DYLVREDGSTYHTFYMDVKDGKPRYGSTHQGYSDNSCWARGQAWGIYGFALSYRYLKE
                     QKYFDLSKILANYFMNRLPEDTICYWDLIFDDSSREEKDTSSMVIMVCGLLELIESGL
                     LDKTEKNLYQSAVWKIMRQLKERYATTEEEDGFLTQSVYNKPKNNGVNTPCIWGDYYY
                     LEALVRMTSNWSSYW"
     gene            complement(6683..8554)
                     /locus_tag="ACG8KR_001769"
     CDS             complement(6683..8554)
                     /locus_tag="ACG8KR_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HFQ0201136.1"
                     /translation="MKHPVPLLRTRQDYFDLFKKINQPLQPFYRASGAQIDYIHQGVG
                     YGNKIAGMEGFSRVLWGAGPAIDQLDERWLKLILSGIKAGTDPSHPDYWGEIHERDQR
                     MVEMPAMLLALYHHEQLLWKKLDTGEKSQIIHWFSQIFEYECADGNWQFFKIIVGTII
                     QKFGYPVKESALKEAFDNIENCYLQNGWYRDSSRGRQDYYNPFAFHYYGLIYSVLEPE
                     KPISQIYKNRAIDFSRDYIHFFAEDGANVPFGRSMIYRFAVSSFWSAMLWADLLPFKL
                     GEIKGLVNRNIRWWMQKEIFDEKGILNIGYTYPQLTLTEPYNSTLSPLWLNKIFLLLA
                     LPEDHEYWTAKEMPMPIREGTKLLSEANLLIQHDNGHTFFLNAGQLGPNFHTLTNEKY
                     LKFAYSSQFGFSIPRANQLKAEMAMDSMLGIQRADTYITTSFKNESIKTYGQFIVRNN
                     VRDIVCKKEVLASTWEPTLQAKIRTWLIPFEGWQIRVHKVELDTEYVLYETGFAIECS
                     PEKEGIIIEEDKENYRVSEAGFSGIICLSDDTIKRKSTAIMGLPNTNLMTWENTFIPG
                     LEGTFGKGTHWLGTGVVAHQDRDYSLEVWNNRPKIDFDGTTGLTIQNKNNKKRLKLC"
     gene            complement(8575..9693)
                     /locus_tag="ACG8KR_001770"
     CDS             complement(8575..9693)
                     /locus_tag="ACG8KR_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFQ0201137.1"
                     /translation="MNEQTKEWSRKILNVASEKIDAELDRMGNKIPYISYNGKYTDVI
                     KDRGIDWWTNGFWSGILWQLFHKTKREKFKEAAIQQEQLLESALYHFSDVHHDVGFLF
                     LPSAIAHYQQSQNKHSRQLGLHAATVLAGRYNISGNYLVAWNDHKPGWMIIDSMMNLP
                     LLYWASRETGDPRFSKIARQHADTTAKYLVRKDGSTGHIAEFDAESGKYIRLLGGQGY
                     NENSCWSRGNAWALYGFALSYRYTKNPYYLAIAEKIAVNFIQKIEKTGYIPLIDFAVK
                     DSNIDTSAGVCAACGLLELAGYVSSVRSKMYHEAAINILQKTEKHYANWNMDEDGIIG
                     GGAEAYHRELTYNVPLIYSDYFFIEGLLRLEQSELAIW"
     gene            complement(9776..10609)
                     /locus_tag="ACG8KR_001771"
     CDS             complement(9776..10609)
                     /locus_tag="ACG8KR_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0201138.1"
                     /translation="MTKQKIQSRIFTYVIIVFGALIFLMPYLYMIVASTQSNQEILSG
                     GINFKFGDMLLKNWQAIMARYNYPLIIWNSIVIAVISTVLATASSTMAGYALAKYRFM
                     GNKLLFNTVMLSRMVPFFTTLIPLFYIMSRLGLSNTYAGVIIPSIASTTSVFMMRQYS
                     SQFPTALMEAARIDGANEWVIFTKIAVPILSPTIITSGLLIFMGSWNQYLFPLVMLSD
                     KDKYTVPLIIKNMTVSSTGEPINYGAIMLILATSVIPMVLIYVWAQSKFKENDIGAAN
                     K"
     gene            complement(10602..11519)
                     /locus_tag="ACG8KR_001772"
     CDS             complement(10602..11519)
                     /locus_tag="ACG8KR_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0201139.1"
                     /translation="MIQSSLPSKTIKNSKKKATDTSHKKSAYGLVTPFVIFFIVTILI
                     PIIISFAFSFMKIGYSFEFVGFDNYLAIFRDPLFIKSYTNVLIIMAGSIPITMLLAIY
                     FAVLLNSAQLKGKGFFRTVYYIPTITSTVAVASVFMTFFNPTGLFNGFLNALGINGVQ
                     WLTNPFWIRISMIITMIWLSVGYNTILFLAGLQGISKEIYESAEIDGASKFRQFFLLT
                     IPMLKPIILMATVLATINGLGNFNIPNIFFGTSNGPENSALVVGVNLYKTSFEMVDFG
                     KASAIAWTMVFVSVILSAIQFKFGGDKND"
     gene            complement(11746..12870)
                     /locus_tag="ACG8KR_001773"
     CDS             complement(11746..12870)
                     /locus_tag="ACG8KR_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFQ0201140.1"
                     /translation="MKKRYKLVIALLNITLLFSLSACGKTNEEVTANIADPMIKKATT
                     LEVYVANGKLTNYVENAAKIYNRKTGANIDLKIVNVASGTATVQMITPKLVTHEDMPD
                     IISMADTSAAGVLEKFQDAFYSADEYGFYDKYGDQFYDQKLNILRQQTSNETIIPWAS
                     DFTPALCYYQPKKFAEIGVDFEEISSWEEYIQVAKALKEKTGSFGIALPESGDQELFI
                     NMLAQQNQSLLDEEGNIRLNTKEAKHAAKLIKEMIDSDIVHFYGAQDAEKAFQESAMF
                     VAGGWYATNMSLNFPDASDKWRMAPLIPFSKENPGKGAVSGGSSFYVPKDAKNPQVAQ
                     QFLTFMLTDEECLANALELGVATSNSLAYQTEAAEKKFDY"
     gene            13053..>13847
                     /locus_tag="ACG8KR_001774"
     CDS             13053..>13847
                     /locus_tag="ACG8KR_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFQ0201141.1"
                     /translation="MVLLLTNNQSSNLVKKTLTYSIDTHLHLSAAPYFRLLINESANL
                     YAEFAHKSIELDNPSLLFIDKQQEVQIFSTQKETQTFIIIQFYFDEQLLAHITGQSFS
                     IDQEITSIITFLKNEQFKIDYLKHSKPAISYDQEINSFFILCQQMSIHLLSLLVTYCL
                     QKIQLSSLPRHYQQELALLTIPSNEKVIPKKKHILYSDELVDPIIRYLEEHLSENFLL
                     EQLAQIFFISPASLKKHFRKVNGKSVISYFKELKMEKAKRLVLENEL"
BASE COUNT         4370 a         2752 c         2259 g         4466 t
ORIGIN      
        1 aattctcttc acaatctttt ccaacacctt tgaaaccgat atcaaatact ttcaggatgc
       61 tgcttctaaa ataaatatca aacaaatgaa tacatcatgc aaaaaaagcc agacaaccga
      121 cgtccttgcg tgtcagctgt ctgagctatg tttatttatt ttgttctttt aaaaagaacg
      181 atacacatac tgatttttcg gagcagagac tttctcagcg tttgtaacaa ctacagatgg
      241 gatttcacgc ttgaatggtt gataatagtc tgattggatg gttccttcta ttttatacca
      301 ttcatcattt ttaaccgtta ctccttccgg catatgcgtc aacaaaccaa agactcctga
      361 atctgccaca caatggataa tgccgaatcg gaacaagaag atatctgtat tgctatcgtt
      421 cggtgtttgg taaacaaatc cttcatagct gatttttttg ccaatgaatt cgctagggta
      481 attataaatt agctccataa cttctaaata gttttcatct gtgatcttga tagtttgctt
      541 atccccatac ttttctttca actcattcat ttggtctaag tagtctgatt tgttgaaata
      601 aatactggta tccggcttta agtattgtgt ctgcatctcc ggatccccta ctgattcttt
      661 acttactggg aaattaaatc ctttggcttc tacgatcgta gtgtcgagac taactgttgg
      721 aaacaacgtc cccacgatta ctggcaaagc tagcagtaca taagcgattc cacgttgata
      781 aggttttgac aacccatggt catgatcgtg ctcgtgtgcc ttttcttctg cttttccgtt
      841 tttgacccac aaatacagct gaactaaagc caaaagagca gataaaatca ttgaaagaat
      901 agctaagtag cgataatgga cattgatata ttgatcgagt ttgccagaaa cttgcaagta
      961 catcatcaat tcgaaatacc cagataaaat caaaaaccgc agcatatttt ccctcctaaa
     1021 taaataatgt gaatccaata acgctaaggg caatcagtcc gatcagcgat aaaataaagc
     1081 gtcctgtaaa atatcgtttc atcatcagta gatttttgat atcgaccatt gggccaaaga
     1141 ccaaaaaggc agctacaggt gctgtgccga acagacttaa caaagaagat cctataaacg
     1201 catctgcttc tgagcaaaga gacatcgtaa aggccaataa cagcatgatg ataatggcta
     1261 aaatctttgt tgagccgagc gttagcataa ttctagttgg taaataagtc tgcatcgcag
     1321 ctgcgattaa tccgccaatg atcaaataac gacctgtatc aaaaaattcg tcgattgaat
     1381 gtgtcaacac atgcccagtt tgatggatca gctgattttt tggttcattc ttctcacttt
     1441 gttcttcttc atggacatga tgatggtgat tttctgctac tttagacgtg attttttctt
     1501 tcaatacagc gtctttttga aaataggcaa gccagacacc aatcacaagg gcaacaaaaa
     1561 agctgcctat cacccgccag cttgccatct gccaagaatt gccaaatgcg ataaaagtgg
     1621 aaaaaatcac gattggattg atgatcggag cagtcagcat aaaagcaaac gctgtgtgaa
     1681 ctggtacgcc ttttttaaca aattgatgaa cgatcggtac gatgccgcat tcacatgaag
     1741 gaaagaaaaa tcctaagatc gaaccagtaa aaatagagaa gatacgattt tcaggaagcc
     1801 atctcttcac acgttctggg gtcaaaaata cttgtaaggc gcctgagata agacagccta
     1861 ataatacgaa aggtaatgct tcaataacga tcgataagaa aatcgtactc atctgcaaaa
     1921 ccgaatttgg aataaaagaa aacatgagtt tcctcctttt tatttctagt tcattcgctt
     1981 tgcaaatata cgcttttatt caaaaaaaac aagttgctct gtaaattttt acacaactct
     2041 tattttttac caaagcactt caattgactt tttctgttat tcccattaaa ttaaggtctc
     2101 atacatatca cttctatcat aaaagggaga ataaaaatga aacaaaccac taacttgact
     2161 gaagttcaag acatactgca acagtcatcc gttgccgtca tttatctttc gatgcctaat
     2221 tgcagtgttt gtcacgctgt tcgtccacga ctagaggaac ttctcacaca ttatgatatt
     2281 ccggcacttc atttggatgc atttgaaaca ccagaagtag ctagtaaatt tgaagtgctg
     2341 actgcgccag ttgtcctgat ttttcatcaa ggaaaagaag tctttcggca agcacggttc
     2401 atcgacttta aaaaaatccg ttatctacta gatgaactga cagcagaaga tgattccctt
     2461 tcttatgaag aaatcttccg aacaaaataa tcgttgtcca aacagaaaaa agcgattgaa
     2521 caagtgaaag ttttgccttc atttgttcaa tcgcttttta tttttattct gctgtatttt
     2581 cggaatgcgc atttgcgtca gaatgcaaga aatagatcaa ggtttgcaat tcattcgtca
     2641 aatcaacatt ttggatcaat acatctgacg gagcattcaa tcgagcagcg gtaaagttca
     2701 agattccttt gattcctgct tccgttaact gattgactac ttcttgtgct ctttcagcgg
     2761 gtatcgttaa aattgctact tcgatttgtt ggatcttgat ttgttctgac atatcttcca
     2821 ttggataaac ggggataccg tctacgatgc gcccaacgat ttcctcattc acatcaaaag
     2881 cacaactgat acgtatgcta ttactttgat ggaatttgta ttttagtaaa gcacttccca
     2941 agttccctac accgatcagc gcaacatttg tcaattgatc gtcgtttaat gtctttgcaa
     3001 aaaagtgcat aagatcttct acatcataac catacccacg ttttcctaat tcgccaaaat
     3061 aagaaaaatc tcgacggatc gttgcactat ctacttgaac ggcttcactt aattcagttg
     3121 aagacacttt tttctttcca gcattattta aaatccttaa atagcgataa tacaaaggca
     3181 gtcttttagc tgtcgccttt ggaataatat gatctttcac gaaatgacct ccacgtattg
     3241 tctaattaca tctttgattt ctccatcata gcagtattta taggaaaaaa tcaattccat
     3301 tgctcagata ttgtgaaatt ttattcttta ttcatttatt tatcatttct gcaaatctcg
     3361 cagtataatg gaatttatgg tagactacta attgaaaaat agttttttat ttgaggagaa
     3421 ttcacaaagt agaaaagagg attccatgat attattacaa gccaatcaga ttgctcgcca
     3481 ttttggtgca gaagtcttat ttgaaaacat acatttagaa atcagtacag gcgctcgtgt
     3541 cgctttagtt ggcagaaatg gcgctggaaa atcgacttta ttaaagatca ttgctggaat
     3601 tgaagcacct gatgaaggaa agatcgctaa aaataaaaca gcgactctcg gatacctcgc
     3661 tcaggacaca ggacttgatt ccaatgaaac agtttggaac gagatgctga aagcttttga
     3721 acaagttaga caaatggaag agcggatgcg cgagctagag attttcatta gcgaaggtac
     3781 gccagacaca cctgcttata gttcttattt aaaagaatac gaccggctgc aacacgattt
     3841 ttcagatcga aacggctatg gttatgaaaa tgaaattcgt tccgtattgc atgggtttaa
     3901 attcgattct tcattttatg atcagaaaat cgatacttta tcaggcggac agcgcacacg
     3961 tttagcttta gctcgcatgc ttttgcaaca ccctgacatt ttgattctag atgaaccaac
     4021 gaaccattta gatatcgaga cactttcctg gcttgagaat tatctgcaaa gctatactgg
     4081 tgcattattg attgtttccc atgaccgtta ttttttagat aaagtagtca atgaagtcta
     4141 tgaattaagc agaagaaaaa tgcgccatta caaaggtaac tattcgaaat atctagattt
     4201 gaaagcagaa cagttagcaa gtgaatggaa ggcttatgaa aagcagcaaa cagaaatcca
     4261 taaattagaa gacttcgttg ctcggaacct tgttcgcgcc tctaccacaa aacgagcgca
     4321 aagccgcaga aagacattag aaaaaatgga ccggctggat cgtcctcaag gggatgaaaa
     4381 acaagcaaac tttctattca gtatagaaaa gccttctgga aatgtagtct tgcaaatcga
     4441 agatgccgct atcggatatg ataatgtcac cttatctgaa ccaatcaatc tagatatccg
     4501 tcgtcaagaa gcgattgctc tagtaggtcc gaatggtatc gggaaatcga cgttgctgaa
     4561 atcaatcatt caccagatcc cttttatccg tggaaaagaa acgctaggga caaatgtcac
     4621 catcggctat tacgatcaag gtcaagctga tctgcataca aacaagacga tcctagcaga
     4681 attgtgggat gagcatccta ccactccaga aaaagagatc cgaagtgctt taggtgcttt
     4741 cttattttct ggcgaagatg tagaaaaacc aatccctctg ctaagtggcg gtgaaaaagc
     4801 acgggttgct ttagccaagt tagcgatgaa caaagaaaat tttttgattt tggatgaacc
     4861 aacaaaccat ttagatattg acaacaaaga agtattagaa aatgcattga tcgattatga
     4921 cggcacactg ctttttgttt cccatgaccg atatttcatc aatcggatcg cgacaaaagt
     4981 cgtcgaactc tctgaaaatg gcagcaaagt ctatctagga gactatgatt attatttaga
     5041 gaaaaagcag gaagaagaag aaatcgccga attgttggca caagatgagc cagaatctgc
     5101 tcctgctccc acgaacaaat ctgattatta ccaaagcaaa gaacagcaaa agcttatccg
     5161 tagccttcaa cgaaaaatta ctcaggttga agaagaaatg gcgcggatcg atgaatggat
     5221 cgatcagttg gaacaagaaa tggtcgcgcc agaaaacctg gaagatcacg taaaactcaa
     5281 tgagctgaac caagatttag aagctgctag acaagatcaa gaaacaaaat tgacggaatg
     5341 ggaagaacta agcttggaat tagaagaata tgaaaattaa cgaaaaaacc aaaacagaaa
     5401 ccattcatgc tgttttggtt tttctttttt tattttatca aacaaaaatt ccatatttac
     5461 ctttttcacc aataagaaga ccaattacta gtcattctca ctaaagcttc taaataatag
     5521 taatcacccc aaatacaagg tgtatttacc ccattatttt tgggcttatt ataaacagac
     5581 tgtgttaaaa agccatcttc ttcttccgtt gtagcatatc tttctttcaa ttgtcgcata
     5641 attttccaaa cagcagactg gtataagttc ttttctgttt tgtctaatag acctgattct
     5701 attaattcta ataatccaca aaccatgatc accatactag atgtatcttt ttcttctctg
     5761 ctagaatcat caaagatcaa atcccaataa caaatggtat cttctggcaa ccgattcata
     5821 aaataattgg ctaatatttt gcttaaatca aaatattttt gttctttcaa ataacgataa
     5881 cttaatgcaa aaccgtaaat cccccatgct tggcctctag cccaacagga attatcacta
     5941 taaccttgat gcgtacttcc atatcttggt tttccatctt tgacatccat ataaaatgta
     6001 tgataagttg aaccatcttc tcttactaaa taatctgctg cattttgaat atgagcctga
     6061 gcagcttttt tatatttttg atttccagtt tcttcactcg cccaatacaa taatggaaga
     6121 ttcatgcaac aatccatgat cattctacct tgttgtgaag gatcactagg atctccccaa
     6181 gcttgtataa ctcctgcctt ctcccaaaaa cgctgaagta atgtttctgc agcatcaatt
     6241 gaagcttcct tcatttttcg atttcccgtt attttataat ctgctacggt tgataaagaa
     6301 tacaaaaaac caagatcgtg atgattagtg ataatttgac agtcaatcct gtggataaaa
     6361 tctatatctg ctttttcagc tgtttgttga aataaaggat cacccgtctt ctcaaataac
     6421 aaccaattaa ttcctgtcca aaatccttct gtccaaccaa tattgtctct taatggataa
     6481 cgtcctttta taggacactc gcttgggaat tgatactgac tgttactgat acgtttgatt
     6541 aacctctccg tgtgtctgcg acaacgctcg atctcattat tccattcttt ttccattaat
     6601 tctttataac attcatttat ccatttttct ggatgtatta tctcttcaaa catcacttta
     6661 tcaagcattt ccccaccagc ctttagcata attttagtct ttttttattg tttttatttt
     6721 ggatagttag cccggttgtc ccatcaaaat caattttggg acgattgttc catacttcta
     6781 aactataatc ccgatcttga tgagcaacca ctcctgttcc gagccaatgg gttccttttc
     6841 caaatgtacc ctctaatcca ggaataaaag tgttttccca tgtcatcaag tttgtattgg
     6901 gcaaacccat aatagctgtg gattttcgtt taatagtatc gtcacttaaa cagataatac
     6961 ctgaaaagcc agcttcactc accctatagt tttccttgtc ttcttctatg attattcctt
     7021 ctttctcagg agaacattca atagcaaacc ctgtctcata tagaacatat tctgtatcta
     7081 attctacttt gtgtacgcga atctgccagc cttcaaaagg aataagccaa gttctgattt
     7141 ttgcctgtaa agtaggttcc catgtagagg caagtacctc tttcttacat acaatgtctc
     7201 gtacattatt tcgaacgatg aactgtccat aggttttgat gctctcattc ttaaaactgg
     7261 tcgtgatata tgtgtccgct cgctgaatcc ctagcattga atccattgcc atttctgctt
     7321 tcaattgatt cgctcgcggt atcgaaaaac caaattgact agaataagca aattttaaat
     7381 atttttcatt ggtcaatgta tggaaatttg gccctaattg tcccgcattc aaaaaaaatg
     7441 tatgtccatt atcatgttgt atgagcaaat tggcttctga aagaagttta gttccttcac
     7501 gaataggcat aggcatttct ttagccgtcc aatactcatg gtcttcaggt agcgccaaca
     7561 ataaaaaaat cttattcagc cataatggag ataacgtact attatagggt tcagtcaatg
     7621 tcaattgagg gtaggtataa ccaatattca ggataccttt ttcatcaaat atttcttttt
     7681 gcatccacca tcttatattc cgattgacta accccttgat ttcacctaat ttaaaaggca
     7741 aaagatcggc ccacaacata gctgaccaaa agcttgaaac tgcaaaacga taaatcatgc
     7801 ttcgaccaaa aggcacattt gcaccatctt ccgcaaaaaa atgaatatag tctctagaaa
     7861 aatcaatagc tcggttctta tatatctggc taatgggctt ttctggttct agtacgctat
     7921 aaatcaaacc ataataatga aatgcaaaag gattataata atcttggcga cctcttgaac
     7981 tgtctcgata ccagccattt tgaagataac aattttctat attatcaaat gcttctttca
     8041 gtgctgattc tttaactgga taaccaaatt tttggataat tgttccgaca attatcttaa
     8101 aaaattgcca attaccatct gcacattcat attcaaaaat ttgcgagaac caatgaataa
     8161 tctggctttt ttctcctgta tctaattttt tccataataa ttgttcatga tgatataacg
     8221 ctaataacat tgcgggcatt tctaccatac gctggtctct ttcatgtatt tctccccaat
     8281 aatcgggatg ggaaggatct gttccagctt ttattcctga tagaattaat ttcaaccatc
     8341 gttcatctaa ttggtcaatt gcgggccccg ctccccataa aacccgagag aatccttcca
     8401 ttcctgcaat tttatttcca tatcctaccc cttgatgtat ataatcaatt tgtgcgccac
     8461 ttgctcgata aaacggctgg agtggttgat ttatcttttt aaataagtca aagtaatcct
     8521 gtctggtcct taatagtggt actggatgtt tcattgtatt ttctcctctt tttactacca
     8581 aatagctaat tccgactgtt ctaatcgaag taagccttca ataaaaaaat aatcagaata
     8641 gatcagtggc acattataag tcagttctct atggtatgct tctgctccgc ctccaatgat
     8701 cccatcttca tccatattcc aatttgcata gtgcttttct gtcttttgga gtatattgat
     8761 agcagcttca tgatacattt ttgatcgtac agaggaaaca tatcctgcta attccagaag
     8821 accgcatgca gcgcatactc cagcactggt atcaatattc gaatctttta cagcaaaatc
     8881 aatgagagga atataaccag ttttttcgat tttctgaata aaatttactg ctatcttttc
     8941 tgctatagct agatagtacg gattttttgt gtaacgataa cttaacgcga atccatataa
     9001 agcccatgca tttcctctgc tccaacaaga attctcattg tatccttgcc cgccaagtaa
     9061 ccgaatatat ttccctgatt ctgcatcaaa ttctgcgata tgcccggttg atccatcttt
     9121 cctcactaga tacttagctg ttgtatctgc atgctgccga gcaattttag agaacctcgg
     9181 atcaccagtt tccctcgaag cccaatacag taatggtaaa ttcatcatac tatcaataat
     9241 catccaacca ggtttatggt cattccatgc tactaaatag ttccctgaaa tattatagcg
     9301 tccagcaagt accgtagctg catgtaaccc tagttgtcga ctatgtttgt tttgagactg
     9361 ttgataatgt gcaatagccg atggtaagaa taaaaaacct acatcatgat gaacgtctga
     9421 aaaatggtac agggcagatt ctaataattg ttcttgttgt attgctgcct ctttgaactt
     9481 ttctcgtttt gtcttgtgaa agagctgcca taaaatacca ctccaaaaac cattggtcca
     9541 ccagtcgatt cctctgtcct ttattacgtc tgtatatttt ccgttgtaag aaatatatgg
     9601 aattttattc cccattctgt ctaattccgc atctatcttt tcagaagcta cattcaaaat
     9661 ttttcttgac cattccttcg tctgttcatt catggatatc ccgccattct tttaattttt
     9721 atgaatgtgg agttgctcct agaacgaact agatggttaa cattcgttat tttttttact
     9781 tatttgcagc tccaatgtca ttctccttaa atttagactg cgcccacaca taaatcaaga
     9841 ccattgggat cactgaagtt gctaaaatta gcataattgc gccataatta attggctcac
     9901 ctgtactaga aaccgtcata tttttgataa ttaagggaac cgtatattta tctttatctg
     9961 aaagcatgac taatggaaat agatattgat tccaacttcc cataaagata agtaaaccac
    10021 tagtaataat cgtaggtgat aaaatcggta cagcgatttt cgtaaaaatc acccactcat
    10081 tggcaccatc gattctggca gcctccatca gtgctgtagg aaattgactg ctatattgtc
    10141 gcatcataaa cactgaagtt gtactggcaa tcgaagggat gatcacacct gcataagtat
    10201 tcgataatcc taaacgactc ataatgtaaa ataaaggaat caatgtggta aaaaaaggca
    10261 ccatcctaga tagcataact gtattgaaca acagtttatt ccccataaaa cgatactttg
    10321 ctaatgcata acccgccatt gtagaactgg ctgtagctaa aactgtactg attacagcaa
    10381 taacaatcga attccaaata atcaagggat aattatatct cgccatgatg gcttgccagt
    10441 tttttaatag catatcccca aacttgaaat tgattccgcc acttaaaatt tcttgattac
    10501 tttgcgttga agctacaatc atatataaat atggcatcaa aaatattaag gcaccaaaca
    10561 caataatgac gtacgtaaaa attctgcttt gtatcttttg tttagtcatt tttatcacct
    10621 ccgaatttaa attgaatagc cgataatata acagatacaa acaccatcgt ccaggcaata
    10681 gcagaggctt ttccaaaatc aaccatttca aaacttgttt tatataaatt gactcctacg
    10741 accaaagctg aattttctgg tccattggaa gtcccaaaga atatatttgg gatattgaaa
    10801 ttacccaagc cattgatagt ggctaaaact gtggccatta aaatgattgg ctttagcatg
    10861 gggatcgtta ataaaaagaa ttgccgaaat ttcgatgctc catctatttc agcactttcg
    10921 tatatctctt tagaaatccc ttgaagacca gctaaaaata aaatagtgtt atatcctaca
    10981 ctaagccaaa tcatcgtgat gatcattgat attcgtatcc aaaaaggatt tgttaaccac
    11041 tgtacaccgt taattcctag cgcattcaaa aatccattga ataacccagt cggattgaaa
    11101 aaagtcatga atacggaagc tacagccacc gttgatgtaa tggttggaat ataatagaca
    11161 gttctaaaaa atccttttcc ttttaactgt gctgaattca ataaaacagc aaagtaaata
    11221 gctaatagca tcgttattgg aattgatcct gccatgataa tcaaaacatt cgtgtaagat
    11281 ttaataaaca atggatctcg gaagatagct agataattgt caaatccaac aaattcaaat
    11341 gagtagccaa ttttcataaa actaaaagca aacgagataa tgatgggaat caaaatcgtc
    11401 acaataaaga aaataacaaa aggtgtgacc aatccataag cacttttttt atgagacgta
    11461 tctgttgctt ttttcttgct gttctttatt gtcttgcttg gtaacgaaga ttgtatcatc
    11521 gttaagtcct cctaatattt tttaatcacc agttaatttt acgccgtact tatgagcaaa
    11581 attttctgct tcttttagat atgaattttc aaaatcacct gtttgccaat agttatatgc
    11641 agcggtagtc aaataagacg tagcatctga ataagtggct ggtaaaacag tatttgatgt
    11701 attttcattg attttattca atagttcata gtatttttgt cctccttagt agtcaaactt
    11761 tttctcagct gcttctgttt gataggccaa tgagttagat gtagccacgc ccagctccag
    11821 tgcgttggct aagcattctt catctgtcag cataaatgta agaaattgtt gtgccacttg
    11881 tggatttttg gcatctttag ggacataaaa acttgatcct cctgatactg caccttttcc
    11941 tggattttct tttgaaaaag gaatcagtgg agccatgcgc catttgtcag atgcatcagg
    12001 aaaattcaaa gacatattcg tagcatacca gccgccagca acaaacattg cagattcttg
    12061 aaatgctttc tcagcatctt gtgcaccgta gaaatgtaca atatctgagt cgatcatttc
    12121 tttgattaac tttgcagcat gtttcgcttc tttagtattc agacggatat ttccttcttc
    12181 atcaagcaaa gattgattct gctgggctag catgttgata aacaattctt ggtccccaga
    12241 ttctggcaag gcaattccaa aactaccagt tttttctttt aatgcttttg ccacctggat
    12301 atattcttcc caagaactga tttcttcaaa atctacacct atttcagcaa atttttttgg
    12361 ttgataataa caaagtgctg gggtaaaatc actagcccaa ggaataatcg tttcattact
    12421 cgtttgttga cgcaaaatat tcaatttttg atcataaaac tgatcgccat atttgtcata
    12481 aaatccatat tcatcagcac tataaaaagc atcttgaaat ttttccagca cacctgctgc
    12541 tgaagtgtct gccatggaga taatatccgg catatcttcg tgtgttacca attttggggt
    12601 gatcatttgt actgttgctg ttcccgaagc tacattcaca atttttaagt caatatttgc
    12661 tccagttttg cggttataga tttttgcagc attttcaaca tagtttgtca gtttgccgtt
    12721 tgcgacatat acttctaatg tagtggcttt ttttatcatt ggatctgcga tgtttgctgt
    12781 tacttcttcg ttagtttttc cacatgcact taagctgaat aatagggtaa tgtttagcaa
    12841 agctatcacc aatttgtatc tttttttcat gtctatccct tctttctttt caagttctta
    12901 tataaaacgc tttcacttat gtatattaac ggaattagta acacaaatat ttatataaaa
    12961 aaatatttta cttgtcgttt tggcttttca tttttgttat aatagtcatt aattataaat
    13021 agaaagcttt ttatataaaa agagaaagga aagtggttct tttgttaaca aacaaccaat
    13081 cctccaatct tgtcaaaaaa acactaacct attccataga tacccatctt catttatctg
    13141 ctgctcccta ttttcgacta ctaataaatg aaagcgctaa tttatatgct gaatttgcac
    13201 ataaatctat agaactagat aatccttccc ttttattcat tgataaacag caagaagtcc
    13261 aaatcttttc tactcaaaag gaaacacaaa cttttatcat tatccagttt tattttgatg
    13321 agcaattatt agcacatatt acgggacaat ctttttctat agatcaagaa ataacatcta
    13381 tcattacttt tctgaaaaat gaacagttca aaattgatta tctaaaacat agtaagccag
    13441 ctatttccta tgatcaggaa atcaattctt ttttcatact atgccaacag atgagcattc
    13501 atttgctttc cttattagtt acttattgtc tgcaaaaaat tcaattgtct tccttaccaa
    13561 ggcattatca acaggaactt gctttattga caataccttc aaatgagaaa gtcattccta
    13621 aaaaaaagca tattctttat tcagatgaat tagttgatcc aatcatacga tatttagaag
    13681 aacatctttc agaaaatttt ttgcttgaac aattagccca aatttttttt atcagtccag
    13741 ccagcttaaa aaaacatttt agaaaagtga atggaaaaag tgttattagc tattttaaag
    13801 aactaaaaat ggaaaaagca aaacgtctcg tgctagaaaa tgaatta
//
LOCUS       DAZKLD010000076        13805 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.076, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000076 DAZKLD010000000
VERSION     DAZKLD010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13805)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13805)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13805
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.076"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            7..174
                     /locus_tag="ACG8KR_001775"
     CDS             7..174
                     /locus_tag="ACG8KR_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HFQ0201142.1"
                     /translation="MKKFVSGILVGSLATAAAVAGLVASVKKTVIDPIDEKEAMIEEN
                     RKKAMRKRVSR"
     gene            complement(236..2047)
                     /locus_tag="ACG8KR_001776"
     CDS             complement(236..2047)
                     /locus_tag="ACG8KR_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HFQ0201143.1"
                     /translation="MKIGIIGAGPRGLSIAERILHNNQDRHSLELFLFDPNGPGGRVW
                     RLNQPPELLMNSVSQQVTLFTDETLSSGGLVTQGPNLFQWSKGPATDYIEQTVTKNKQ
                     LIIEEINRLEKDQQSTRCLYGLYQRWFFSKLQEEFPASIQFVYSLVKKVSKRKDGYLL
                     QTDQQSYSIDQLVLASGHWENDETEEERVWVDYAKKNDLLYQLPSNPADVDLSKIPPK
                     ENVFLRGLGLSFFDYVGLFTVSREGRFENEKGKLVYHPSGKEPIVYSGSRRGLPYYPR
                     GRNQKQGGSMAWPRLLTKENLEQWHQSGFLTGETFFDYLKKDVELFYYKKIIEEYHLP
                     IPKKNFEHDFLSLSSEECLGKHPALIPYRWDWSYLEIPLTYQDESFAEASRAFIEGQI
                     KEAEKGNCTGAFASTFDALKDWRDPIRQAIEWHVFSAKEYKEILWGWFTHLNSFLTIG
                     PPVIRTKELAALIDAGIFHLFEPPFDIQQKHNRYVISLQDKEISSRYLIEARLPQTSF
                     SRSSNPVIKNLKENKLVRPFSFSDNTGSYHSEAIDVERGTNRVIDASGEIQSDFYCYG
                     IPVEGVDWLTAAVARPYTDPWNLRQADKIAQLILTDK"
     gene            complement(2044..2409)
                     /locus_tag="ACG8KR_001777"
     CDS             complement(2044..2409)
                     /locus_tag="ACG8KR_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HFQ0201144.1"
                     /translation="MIKLKRVYLDAEKEDGYRILVDRFWPRGISKEKAQLDLWLKEIA
                     PSNEARKTFDHVPERFASFAKKYQQELIFEEEKKQAIFKLSQLMKEHPVITFVYGAKN
                     EHQNHAVLLKKFIEMEACK"
     gene            complement(2572..2967)
                     /locus_tag="ACG8KR_001778"
     CDS             complement(2572..2967)
                     /locus_tag="ACG8KR_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFQ0201145.1"
                     /translation="MVLWVINIILLLIVLAIIFWEVYLRVMAKRSATTLTEEEFREGM
                     RKAQVIDVREKDVFDAGHILGARNIPYTVLKDSLGSIRKDQPVYIYDQKKSLSIRTAN
                     KLRKAGYQEIYLLKGGYDGWSGKIKSKKV"
     gene            complement(2985..3947)
                     /locus_tag="ACG8KR_001779"
     CDS             complement(2985..3947)
                     /locus_tag="ACG8KR_001779"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0051156 - glucose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HFQ0201146.1"
                     /translation="MDKKIIGIDLGGTTAKFAILTPEGEIQQKWSIDTNILDDGKHIV
                     PEIIESINHRLNLYNMKAEDFIGIGMGTPGSVDSEAGTVIGAYNLNWTEVQFVKKLIE
                     AGTGIKFAIDNDANVAALGERWKGAGENDPDVVFVTLGTGVGGGIVAGGNLIHGVSGA
                     GGEIGHITVDPEGFECTCGKRGCLETVSSATGVVRLGRYLAEEYAGDSKLKAMLDNGE
                     EVTSKDIFEMAQEDDPFALMVVDRVCFYLGLACANLGNTLNPSSIVLGGGVSAAGEFL
                     RSRVEKYFKEFTFPQVRESTKIKLAELGNEAGVIGAASLALKFA"
     gene            complement(3947..4159)
                     /locus_tag="ACG8KR_001780"
     CDS             complement(3947..4159)
                     /locus_tag="ACG8KR_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HFQ0201147.1"
                     /translation="MESLYDVQQLFKKFGIFIYVGARIYDIELMTIELRNLYDSRLID
                     HDTYMTAWRILKREHRIEESRKKGNL"
     gene            complement(4180..4878)
                     /locus_tag="ACG8KR_001781"
     CDS             complement(4180..4878)
                     /locus_tag="ACG8KR_001781"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HFQ0201148.1"
                     /translation="MNYQQQIKLRAWLRRPLWTYAFLGIQTAVFLMMELFPRLEIPYY
                     AGMYGPYLVHFNEWWRLVTPIFIHFGLMHFVMNSLILYFMGQQIEAIYGHWRFFLIYM
                     FSGIMGNTASFAFNEANVLSGGASTSIFGLFGALFILGFHFKHNPAIQQLVRHFLLFI
                     VLTFVFGLLDTSVDVWGHVGGLIGGLVLGNVLGLPKQQTSYSIHQRILSTLVFVFLFV
                     ICILLGLKKYGLLV"
     gene            complement(4898..5440)
                     /locus_tag="ACG8KR_001782"
     CDS             complement(4898..5440)
                     /locus_tag="ACG8KR_001782"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HFQ0201149.1"
                     /translation="MDQLKVRLRRKGIEKLKNLAKSGKKKEKEQRIYKQLFASEYWKS
                     AKVIGTTLSNEFELDTQPVIQRAEKEQKKIVVPKTLPKRQMAFYEMDEQSVLVRSSFG
                     VLEPQSEKLYLPEMIDLLIVPGIIYNRAGYRIGFGGGYYDRYLNNFHKKTCSLVFREQ
                     LNEQWQPESFDQKIQQLFID"
     gene            complement(5572..6576)
                     /locus_tag="ACG8KR_001783"
     CDS             complement(5572..6576)
                     /locus_tag="ACG8KR_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFQ0201150.1"
                     /translation="MKKIYWTFYFLLFVLVILSIGSLMIGTPFIYLDQLFATFIGKES
                     SSLALIVREFRVPRLLISLLAGACLGVSGYILQGVTRNELADSSILGINAGAGFLVML
                     YLGFFSHYSLPFLLLVVAFLGGVLAACCVYLAAYDRNGFLGMNRILLSGIAVNAGLSA
                     LTLLCTIQLSKENYGFVNAWLAGSIWGASWPYVWALLPWAVILIPLGGLYAQRIGLLS
                     FGQERAQGLGLNVAKSQKILLLLAVALASGSVAVTGSLSFVGLLAPHAAKLVVRKENN
                     WTFVLSGLFGSIFLLSADMVARVILPSGEVPTGILIALFGAPYFLYALFIRQKHALNS
                     "
     gene            complement(6573..7562)
                     /locus_tag="ACG8KR_001784"
     CDS             complement(6573..7562)
                     /locus_tag="ACG8KR_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFQ0201151.1"
                     /translation="MRTMKKNRLLLLVCFVLLIIFFVALSNGAATVGKADFWQALFDF
                     DRSKQSHQIIRNIRLPRVAGAFLVGSCFALSGALMQGVTRNPLADSGLLGINAGASLA
                     MALSFAFWPKASDFSVFLMSLAGSFAVTLLVFGFLQFSKMGMNPLQLILAGVAISSFF
                     TAISQALTQFFNLQQDLAFWFVGGAANITWKQLAVLFPLYILAVLVSFSLGKAISLIH
                     LSETHALALGSHPKRTRLIVFCLVAVLAAIAVSLVGPVSFIGLLVPPVVRGMVGTDYR
                     YVLPVSFLVGGGLVMLADFTARMINPPFETPFGLVIALIGVPFLLIQVRREQR"
     gene            complement(7559..8365)
                     /locus_tag="ACG8KR_001785"
     CDS             complement(7559..8365)
                     /locus_tag="ACG8KR_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0201152.1"
                     /translation="MTRLRAADIAAGYENKKIIENMSVTIPEGKITSLIGPNGSGKST
                     LLKSFTRILPVKSGKIIVNNSDISTYSSKELAKKITLLAQTSEHPTGMTVEEVVSYGR
                     YPYQKLFSGMDTQDHEAIEWALDATGLAGFRMRDIATLSGGQTQRVWIAMALAQETDI
                     LILDEPTTFLDPAHQLEILQLLTEINQMKQTTILMSIHDINHASRFSDYLLAMKNGKL
                     ITEGTPEEVITKECMKKIFEIDATIVTLPRTNKPVFLTYDLLPKEQEDQL"
     gene            8531..9487
                     /locus_tag="ACG8KR_001786"
     CDS             8531..9487
                     /locus_tag="ACG8KR_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFQ0201153.1"
                     /translation="MKLKTKVFTLSSILLLAGVLSACSPSSDKETATTANSTTSSNST
                     HTVTDTLGHKVEIPNAPKRIIGSYLEDYLIALGEKPIAQWTVGSGSIQDYLQDDLKDV
                     PTISYDLPYEKVLSFEPDLLLISSSATVEGGKYEQYSKIAPTYVVKNGTDVTWEEQLK
                     DVGKALAKEKEAEKIISDYQKSIKKTREELADKIENKTAAVLWVTNNSAFMVSKNRSS
                     GRIVYDDLEFGVPDLVEEVSKKATSDWSAVSSEKLAELDADYIILVNSDKDAAMFNEP
                     SWKNLKAVTDNHVLQFGPESSWLYNGPIASQEMVNDIKEKLN"
     gene            complement(9564..10082)
                     /locus_tag="ACG8KR_001787"
     CDS             complement(9564..10082)
                     /locus_tag="ACG8KR_001787"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0005542 - folic acid binding [Evidence
                     IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HFQ0201154.1"
                     /translation="MFISMWAQDKNGLIGKNGLLPWRLPNDMRFFREHTMDRILVMGR
                     KTYEGMGDLSLPYRHIIVLTTQPNFRTKENAEVMHSIDELLSYSQTISEDIYVSGGSR
                     IFQELLPYTGRIWRTLIDSTFEGDTYIGNIDFSDFALIEEYEGITDQENLYAHRFQKW
                     ERVKNRVASKEE"
     gene            complement(10170..10319)
                     /gene="rpmG"
                     /locus_tag="ACG8KR_001788"
     CDS             complement(10170..10319)
                     /gene="rpmG"
                     /locus_tag="ACG8KR_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFQ0201155.1"
                     /translation="MRVNITLECTSCKERNYLTSKNKRNNPDRLEKQKYCPRERKVTL
                     HRETK"
     gene            10547..10993
                     /locus_tag="ACG8KR_001789"
     CDS             10547..10993
                     /locus_tag="ACG8KR_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HFQ0201156.1"
                     /translation="MPWNMKDFPASMKNLDKLTRKKAIDIANALLDEGYPDNRAIPIA
                     IDQAKKWYEDASESEKRTFEKEKNPSKTDEHDTNPRAGKLLDSDVIVEYEEEQWIVKS
                     KGAKKASNHFDTKKEAIEKGKQVAQNKESTLFIYKKDGTKEKEIDY"
     gene            complement(11188..13083)
                     /gene="asnB"
                     /locus_tag="ACG8KR_001790"
     CDS             complement(11188..13083)
                     /gene="asnB"
                     /locus_tag="ACG8KR_001790"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HFQ0201157.1"
                     /translation="MCGIVGFVNDKDNKKTIINTMMDRIVHRGPNSSGEYIDKHVALG
                     FRRLSIIDLEGGTQPIYNEDRTKIIIFNGEIYNYQPLREELIAAGHVFQTHADTEVLL
                     HGYEEWGTELLQKIRGMFAFAIWDNEKNELFGARDHFGIKPYYYAEMNGTFMFGSEIK
                     SFLPHPDFNKELNKEALKPYMTFQYSPLNGETFFKDVYRLPEGHYYTYKDGKLDIQQY
                     WDADFETKETHSRQEWIEKIDETVQASIEAHTVSDVEVGSFLSSGVDSSYVTSVLKPD
                     HSFSIGFDDKTYNEAIEARKLTELLDLDNTAAVIDGDMSFKAFPLIQYHLDEPDSNPS
                     CVPLYFLANLASQSVRVVQSGEGADELFAGYQTYGFHTNSKFIRVIAQGLKKLPKGTR
                     YNLGRKIGKMKNFHGRIHLYESLAPAKEYFIGHARVFEESEASEVLTPKYQTAPSVDE
                     IMTVHYEKTEGIKDEVNKMQYVDLHQWMPKDILLKADKLSMASSLEVRVPLLDIEVMK
                     LAQQIPSKFLLNQNNTKDIFRQAANKHLPEEWSNRVKLGFPVPIKAWLKEEHGYEQVK
                     ALFEADFAKEFFDQEKIMKLLDDHHEGRKEEQRKIWTIFSFLTWYKVYFVDETIPQAE
                     AIDYVTV"
     gene            complement(13408..>13805)
                     /locus_tag="ACG8KR_001791"
     CDS             complement(13408..>13805)
                     /locus_tag="ACG8KR_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
                     /protein_id="HFQ0201158.1"
                     /translation="NHFSNQISLNVYSRGMGGLGLPGDLSSVSRFVKATFTKMNAASG
                     DSESESISQFFHILGSVEQQKGVCDTGEGKYEYTIYSSCCNVDKGIYYYRTYEDSQIT
                     AVNMNKEDLDRHELVSYPVIKEQQINYVN"
BASE COUNT         4307 a         2921 c         2349 g         4228 t
ORIGIN      
        1 gattcaatga aaaaatttgt atctggaata ttagtaggaa gtctggcaac agccgctgcc
       61 gttgctggtt tggtcgcttc tgtgaaaaaa acagtgatcg atccaattga cgaaaaagaa
      121 gcaatgatcg aagaaaaccg taaaaaagca atgcgcaaac gcgtttctcg ttaataatta
      181 tttaaaaaca cgcatgaaga cttgtgaaag aacagataag tcatcatgcg tatttttatt
      241 tgtcagtcaa tataagttgt gctattttat ctgcctgtct cagattccat ggatctgtat
      301 aaggtctagc tactgctgca gttagccaat ccacaccttc aacaggtatt ccatagcaat
      361 aaaagtcaga ctggatttcc cctgaagcat caatcacgcg attcgtacct cgctcaacat
      421 ctatagcttc tgaatgataa cttcctgtat tatctgaaaa tgaaaaaggg cgaacaagtt
      481 tgttttcctt caaatttttt atgacaggat tacttgaacg agaaaagctt gtttgtggca
      541 atcgagcttc aatcaaatag cgactgctga tttctttatc ttgtagagaa ataacatagc
      601 ggttgtgctt ttgctgaata tcaaatggcg gttcgaacaa atgaaaaata cccgcatcta
      661 tcagtgctgc taattctttc gtccgaatga ctggcggacc gatcgttaaa aagctattta
      721 gatgagtaaa ccatccccat agtatttctt tgtattcctt tgcagaaaaa acgtgccatt
      781 caatagcctg ccggattgga tcacgccagt cttttaatgc atcaaatgtt gaagcaaatg
      841 ctcctgtgca attgcctttt tccgcttctt ttatctgccc ttcaataaat gctcggctag
      901 cttctgcaaa ggactcatcc tgataggtaa gaggaatttc taaatacgac caatcccatc
      961 tataagggat tagtgctgga tgctttccga gacactcctc tgaagagagg gagagaaaat
     1021 catgctcaaa atttttcttt ggaatcggca aatgatattc ttcgatgatt ttcttgtaat
     1081 aaaacaactc aacgtctttt ttcagatagt caaaaaatgt ttcgccagtc aaaaatcccg
     1141 actgatgcca ttgctccagg ttttcttttg ttagtaaccg tggccatgcc attgatccgc
     1201 cttgcttttg gtttcttcct cgagggtaat aagggagccc ccttcttgat ccactgtata
     1261 caatcggttc cttgcctgat ggatgataga caagttttcc tttttcgttc tcgaagcgcc
     1321 cttctcttga tactgtaaac aatccgacat aatcaaaaaa acttaagcct agacctctta
     1381 aaaagacatt ttctttaggc ggtatcttcg aaagatcgac atctgcagga ttagaaggaa
     1441 gctgatacag taaatcattt ttctttgcgt agtctaccca cactctttct tcttctgtct
     1501 catcattttc ccagtgaccg cttgctaaaa caagctgatc gatagaatag ctttgctgat
     1561 ctgtctgtaa aagataacca tcttttcttt tgctgacttt ttttactaaa gaataaacaa
     1621 actgaataga agctggaaat tcttcttgca actttgagaa aaaccagcgt tgatagagtc
     1681 cgtataaaca tcgagtagat tgctgatctt tttccagacg attgatttct tctataatta
     1741 gttgcttatt tttggttacc gtctgttcga tataatctgt agcaggtcct ttactccatt
     1801 gaaacaagtt gggtccctgt gttaccaatc ctccactgga caatgtttca tctgtaaaaa
     1861 gcgtcacttg ttgagaaaca gaattcatta ataactccgg tggctgattc aaccgccaaa
     1921 ctcgacctcc aggaccattg ggatcgaaaa gaaaaagttc aagagaatgt ctgtcctgat
     1981 tattatggag aatcctttct gctatactta atcctctagg tccggcacca atgatcccta
     2041 ttttcatttg catgcctcca tttcgataaa ttttttcaat aaaacagcat ggttctgatg
     2101 ttcatttttt gcaccataaa caaaagtaat gactggatgt tctttcataa gttgactgag
     2161 tttaaaaata gcttgcttct tttcttcttc gaaaatcaat tcttgctggt atttctttgc
     2221 aaatgaagca aatcgttctg gtacatggtc aaaagttttg cgtgcttcat tactaggtgc
     2281 aatttctttc aaccacagat ctaactgcgc tttttctttt gaaattcctc gtggccagaa
     2341 cctatccacg agtattctat agccatcctc tttttcagca tcaaggtaaa cacgttttaa
     2401 tttgatcata gttgtttcct tctttctgcc ttttcgctta gcttatatat gtagataata
     2461 gcacaaaata aaaaaaatga acggcacaac tatccattgc gccgttcatg tgatcttggt
     2521 caaaatctaa tgattgattc ggtttttaaa gctgtcatac aatcatctgt tttatacttt
     2581 tttacttttg atttttccag accatccgtc atatccaccc ttaagtaaat aaatctcttg
     2641 ataacctgct ttacgtaatt tatttgctgt acggatgctc aagcttttct tttgatcgta
     2701 aatatagaca ggctggtctt ttcgaataga tcctaatgaa tctttcaaaa cagtgtaagg
     2761 gatgtttctt gcacctaaaa tatgcccagc atcaaacaca tctttttcgc gaacatcgat
     2821 tacttgtgct tttctcattc cttctcgaaa ttcttcttct gtcaaagtcg ttgctgaacg
     2881 cttggccatt actcgtagat agacttccca aaagatgatt gcaagcacaa tcaatagcaa
     2941 aatgatatta attacccata aaaccatgta atgcctccgt ttttttaagc aaatttcaag
     3001 gctaatgagg cagcaccaat gacaccagct tcattaccaa gctctgctaa tttgattttt
     3061 gttgattctc ttacttgcgg gaaagtaaat tctttaaaat atttctctac acggctacgt
     3121 aaaaattctc ctgctgccga tactcctcca cctaacacaa tacttgacgg attcaatgta
     3181 ttccccaagt tggcgcaagc taaacctaag tagaagcata cgcgatcaac aaccatcaat
     3241 gcaaacggat cgtcttcttg agccatttca aagatatctt tacttgttac ttcttcaccg
     3301 ttgtctaaca ttgctttcag ttttgaatct cctgcgtatt cttctgccaa gtaacgacct
     3361 agacgtacca caccagtagc gctagacaca gtttctaaac atccgcgttt accgcaagta
     3421 cattcgaacc cttcaggatc cacagtgata tggccgatct ctccgcctgc tccgctcacg
     3481 ccgtgaatca aatttccgcc agcaacgata ccaccgccaa cacctgttcc tagagtaacg
     3541 aatacaacgt ctggatcgtt ttctccagca cctttccatc gttcacctaa agcagcaaca
     3601 ttcgcatcat tatcgatagc gaatttgatt cctgttcctg cctcgatcag ttttttcaca
     3661 aattgaactt ctgtccagtt taagttatac gcaccaataa ccgttccagc ttcgctgtcc
     3721 acgcttcctg gggtacccat gccgattccg ataaagtctt cggctttcat attatataaa
     3781 ttcaaacgat ggttgattga ttcaatgatt tcaggaacga tatgcttacc atcatccaaa
     3841 atattggtat cgatgctcca tttttgttga atctctcctt ctggtgttaa aattgcaaat
     3901 tttgctgttg ttccacctaa atcaatccca ataatcttct tgtccattac aaatttcctt
     3961 tctttcggct ttcttcaatt cggtgctctc tttttaatat gcgccacgct gtcatgtatg
     4021 tatcatgatc aatcaaacgg ctatcataca gattacgcaa ttcgatagtc attaattcaa
     4081 tgtcatagat gcgtgcaccc acatagataa agatgccaaa ttttttgaac agctgctgta
     4141 catcatataa gctctccatc tttaagcact tccttcatat tatacaagta aaccatattt
     4201 ttttaaaccc agtaaaatac atattacaaa aagaaataca aaaactagtg tagaaagaat
     4261 cctttggtgt atcgaatagc ttgtctgttg tttcggtaga cccaaaacat tccctaacac
     4321 taatccgcca attagcccac cgacatgtcc ccaaacatct acagaggtat ccagaagacc
     4381 aaaaacaaat gtcaacacaa taaataaaag aaaatgtctg actagctgct ggatcgctgg
     4441 gttatgttta aaatgaaacc ctaagataaa caacgcaccg aacaaaccga atatcgaagt
     4501 actcgcacca ccagacagaa cattcgcttc gttaaaagca aagcttgctg tatttcccat
     4561 tatcccgctg aacatataaa tcaagaaaaa acgccaatgg ccatagattg cctcgatttg
     4621 ctgccccata aaatacagta tcagtgagtt cattacgaaa tgcatcagtc caaaatggat
     4681 gaagattggt gtcactaaac gccaccattc attgaagtga acaagataag gaccatacat
     4741 tccggcataa taagggatct caagtcgcgg aaataattcc atcatgagga agaccgctgt
     4801 ctgtatccct aaaaaagcat aggtccataa cggtcgtctt aaccatgcac gcaattttat
     4861 ttgctgttga taattcatga aaacctccca tttcatttta gtcaataaac aattgttgga
     4921 tcttctgatc aaagctctcc ggttgccatt gttcattcaa ttgttcacga aaaacaaggc
     4981 tacatgtttt tttatgaaaa ttatttaaat accggtcgta ataaccaccg ccaaaaccga
     5041 ttcgataacc ggctctattg tagataatac ctggtacaat gaggagatcg atcatctccg
     5101 gcaaatagag cttctctgat tgtggctcaa gaacaccaaa actcgaacgt acaagaacgg
     5161 actgttcatc catttcataa aacgccatct gacgtttcgg caaagtttta ggtacaacta
     5221 ttttcttttg ttctttttct gctcgttgga taaccggctg tgtatccagt tcaaattcat
     5281 tcgataaagt cgtccctatc acttttgctg acttccagta ctcgctggcg aatagctgtt
     5341 tatagattcg ctgttctttt tcctttttct tgccactttt ggccagattt ttcagctttt
     5401 cgattccttt ccggcgtaat cttactttca attggtccaa aaactcaccc ttccttctct
     5461 cataaaatgg tataggaaaa aaagaaaaat agcaatcgaa ctttcaaata aatgcataaa
     5521 tagaataaaa agaagaccta gaaatgattg gccttccttt tttaataaat ttcaactgtt
     5581 cagcgcgtgt ttttgtctga taaaaagtgc atacagaaaa tatggcgcac caaaaagggc
     5641 aatcaaaatt cctgtaggta cttccccact tggtagaatc actctagcaa ccatatctgc
     5701 actaagcaga aaaatggaac cgaacaaacc gctcagaaca aaggtccagt tattttcttt
     5761 acgtacgact agcttcgcag catgaggagc tagtagtcct acaaaagata aactacctgt
     5821 caccgctaca ctaccgcttg ccaaagcaac agctaacaac aaaaggattt tttgactttt
     5881 agccacgtta aggcctaatc cttgcgcgcg ttcttgacca aatgatagca atccaatcct
     5941 ctgtgcataa agtcctccta gcggaattaa gataaccgcc catggaagca atgcccaaac
     6001 ataaggccag ctcgcacccc agattgatcc cgctaaccat gcattcacaa atccataatt
     6061 ttccttagat aattggatcg tgcatagcaa tgttaatgca gaaaggccgg cattaacggc
     6121 aataccggac aataagattc gattcatacc caaaaagcca tttcgatcat aggccgcaag
     6181 atagacacag caagcagcta aaacaccacc tagaaaggct acaactagca atagaaacgg
     6241 cagcgaatag tgcgaaaaga aacctaaata aagcatcact aaaaatcctg ctccggcatt
     6301 aatgcccaat atactagaat ctgccaattc atttcttgtt actccttgca agatataacc
     6361 agaaacgcca agacaagctc ctgctaataa actgatcagt aatcgaggaa ctcgaaattc
     6421 tctaacaatc agcgccaaag aagatgattc tttccctatg aacgtggcaa aaagctgatc
     6481 caggtaaata aaaggcgtac caatcattaa actacctata gacaggatga caagtacaaa
     6541 aagcaagaaa tagaacgtcc aatatatttt tttcaccgtt gttccctcct tacttgtatc
     6601 agtaaaaacg gaacgccaat caatgcaatg actaatccaa aaggtgtttc aaaaggggga
     6661 ttgatcatcc tcgctgtaaa atccgccaac ataaccaatc caccgccaac taaaaaagag
     6721 acaggtagaa cataacgata gtccgttcca accattccgc gaacaactgg cggaaccaac
     6781 agcccaataa atgaaacagg tccgactaac gaaacggcaa tggctgctaa cactgcaact
     6841 agacagaaaa caatcaaccg cgttcgttta ggatgacttc caagagctaa tgcatgtgtt
     6901 tcactcaaat gaatcaaaga tattgctttt cctaatgaaa aagaaaccaa aacagcaagt
     6961 atatataaag gaaacaatac tgctaattgt ttccatgtga tattggcagc tccgcctaca
     7021 aaccaaaaag caagatcctg ctgcagatta aagaattggg taagtgcttg gctgatagca
     7081 gtaaaaaaag aactaatagc tacgccagct aaaatcagct gtaaaggatt catgcccatt
     7141 ttcgaaaact gcaaaaaacc aaatacgagc aaagttacag caaaagaacc tgccaatgac
     7201 attagaaaca cagaaaaatc agaagcttta ggccaaaaag caaaactaag ggccattgcc
     7261 aacgaagcac ccgcgttgat tcctagtaag cctgaatctg ctaaaggatt tcttgtcact
     7321 ccttgcatca atgcaccgct caatgcaaaa caactcccta caagaaatgc tcccgccaca
     7381 cgaggcaaac gaatatttcg tatgatttgg tgactttgtt ttgatctatc aaaatcaaac
     7441 aacgcctgcc aaaaatctgc ttttcccaca gtcgctgcgc catttgaaag agcaacaaaa
     7501 aaaataatca aaagaacaaa acagaccaaa agcaggagtc tatttttctt catagtcctc
     7561 ataattgatc ctcctgctct tttggcaaca aatcataggt tagaaaaact ggtttatttg
     7621 ttcttggaag cgtgacaatc gttgcatcaa tctcaaatat ttttttcata cactcttttg
     7681 tgatcacttc ttcaggtgta ccttctgtga tcaacttacc gtttttcatt gctaataaat
     7741 aatctgaaaa acgtgaagca tgattaatgt catgaatcga catcaagatc gttgtttgtt
     7801 tcatttgatt gatttcagtc aataactgta ggatctctaa ttggtgtgcc ggatctaaaa
     7861 atgttgtcgg ttcatccaag atcaagatat ctgtttcctg agcaagcgcc atagcgatcc
     7921 agacacgctg ggtttgtcca ccagacagcg ttgcgatatc ccgcatacga aatccagcta
     7981 gacctgtagc atccaacgcc cactcgattg cttcatgatc ttgcgtgtcc atgccactga
     8041 acagtttttg ataagggtat cgtccgtaag atactacttc ttctactgtc atacctgtag
     8101 ggtgttcact agtttgtgct aataatgtaa ttttttttgc aagttctttt gaactgtatg
     8161 tagagatatc agagttattg acaatgatct ttcctgattt cacaggtagt atacgggtaa
     8221 aacttttcaa taaagtagat tttccacttc catttggccc aatcaaacta gtgattttcc
     8281 cttcaggaat cgtaacagac atgttttcaa tgattttttt gttttcgtag ccggctgcaa
     8341 tgtcagctgc tcttaatctc gtcatttcac ccatcctttc gctgaaaatc attctcaatt
     8401 atgacgctgt ttcaacattt cgtcaagcgc tactttcata tctatagcca aatgaagcgg
     8461 ttgacagcgt tcgaaatatc aggaataata accaatgaga aagattctca attatgttag
     8521 gaggaaagaa atgaaattaa aaacaaaagt attcacatta tctagcatat tgctgctggc
     8581 tggagtactg agcgcttgct ccccctcttc agataaagaa acagctacaa ctgccaattc
     8641 gacgacttca tccaatagta ctcatacagt cacagataca cttggacata aagtagagat
     8701 tccaaacgcg cccaaacgga tcatcggcag ttacttagaa gactatctga ttgcactagg
     8761 ggaaaaacct atcgcccagt ggactgttgg cagtggttcc attcaagatt atctgcaaga
     8821 tgatttaaag gatgtcccaa cgatttctta tgaccttcct tatgagaaag tgttaagctt
     8881 tgagcctgat ttgctgttga tttcttcttc cgctactgtc gaaggcggga aatacgaaca
     8941 atatagcaaa atcgcgccaa cttatgttgt aaaaaacgga acagatgtca catgggaaga
     9001 acaactaaaa gatgtcggca aagctttggc aaaagaaaaa gaggcagaaa aaatcatttc
     9061 cgattatcaa aaatccatca agaaaactcg agaagaacta gcagataaaa tcgagaacaa
     9121 gacagctgca gtgttgtggg tgaccaacaa ctcagccttt atggtcagca aaaatcggtc
     9181 aagtggaaga atcgtatacg acgatctgga atttggtgtt cctgatctag tggaagaggt
     9241 ctccaaaaaa gccacttctg actggtctgc tgtttcctct gaaaagctag ctgaattaga
     9301 tgctgactac atcatccttg tgaacagtga caaagatgct gccatgttca atgaaccaag
     9361 ctggaagaat cttaaagcag tgacagacaa tcatgtactg caattcggac ctgaatctag
     9421 ttggctatat aacggaccta ttgccagtca ggaaatggtc aatgatatca aagaaaaact
     9481 aaattgattt taactaaaaa aagaatcttc acgattagtc gtgagatgct ttttttctta
     9541 ttaacaaact agacgcatag attttattct tcttttgaag caaccctatt ttttactctt
     9601 tcccattttt gaaaacgatg ggcatataga ttttcttgat ccgttatccc ctcatactcc
     9661 tctataaggg cgaagtctga aaaatcaata ttcccaatat acgtatcgcc ttcaaacgtg
     9721 ctatcgatca atgttcgcca gatccgacca gtgtaaggaa gaagttcttg aaaaatccga
     9781 ctaccacccg aaacatagat atcttctgag atcgtttgac tatacgacaa aagttcatcg
     9841 atcgaatgca ttacttccgc attttctttc gtgcgaaaat ttggttgcgt tgtcaacacg
     9901 atgatatggc gataaggaag tgaaagatcc cccattcctt cataggtctt tcttcccatc
     9961 actaaaattc gatccattgt atgttcgcga aagaaacgca tatcatttgg caatcgccat
    10021 ggtaacaaac catttttgcc gatcagacca tttttatctt gtgcccacat ggaaataaac
    10081 atattatccg ctccttatga ttttagatgt tttgagataa aaaaagtctc aattctttga
    10141 ccagcaagga attgagaact ttaaaaacat tattttgttt cacggtgcaa agtaactttg
    10201 cgttcgcgtg ggcaatattt ttgtttttcc aaacgatcag gattgttacg tttgtttttg
    10261 cttgttagat agttacgttc tttacaagaa gtacactcta aagtgatgtt tacgcgcatg
    10321 tttttccctc ccatttttac aagaatcctc taagatgact ctcagacttg attatcttac
    10381 catgattttt tctagattgc tagtgttttt cacgattttg ttaagtaaaa ctacttgacg
    10441 ataaatacaa cggttttact tctcaaacaa aatctttttc tttttgtata aataccctta
    10501 cacttgttta tatagattat gttttttaat aaagggggat actaatatgc catggaacat
    10561 gaaggacttt cctgcatcaa tgaaaaactt agataaattg acaagaaaaa aagcaatcga
    10621 catcgcaaat gcattattag atgaagggta ccctgataac cgtgcgatcc caattgccat
    10681 tgatcaagca aaaaaatggt atgaagatgc tagtgaatca gaaaaacgaa ctttcgaaaa
    10741 agaaaaaaat ccaagcaaga cggatgaaca cgatacgaat cctcgtgctg gaaaactgct
    10801 tgattcagat gtgatcgtag aatatgagga agaacaatgg atcgtgaaat ctaaaggggc
    10861 aaaaaaggca agcaaccatt ttgatacaaa aaaagaagca atcgaaaaag gaaagcaagt
    10921 agcacaaaac aaagaatcaa ctttgttcat ttataaaaaa gatgggacaa aagaaaaaga
    10981 aatcgattat tgaacatatg taaaggggtt gtgacgaaaa tcgtgtcaca acccccttat
    11041 gttcgaaaca atggtgttcc tctgccaact ccgaaccata agtcataata tctataaaac
    11101 gggaagagct gttttttgaa attgtttctt atcactcagt gttaaacggt aggagaaaac
    11161 ctctttttta ttgatatttg attttcatca aacagtcaca tagtcgattg cttctgcttg
    11221 cggaatcgtt tcatctacaa agtagacttt ataccatgtc aagaaactga agattgtcca
    11281 gatttttctt tgctcttctt tacgtccttc gtgatggtca tctagcaact tcatgatttt
    11341 ttcttgatca aaaaattctt ttgcaaaatc tgcttcgaat aatgctttga cttgttcgta
    11401 gccgtgttct tctttcaacc aagctttgat tggcacaggg aatcctaatt tcacacgatt
    11461 cgaccattct tccggaagat gtttgttcgc agcttgtctg aaaatatctt ttgtgttgtt
    11521 ttgattcaat aaaaacttac ttggtatttg ttgagccaat ttcatgactt cgatatccaa
    11581 taaaggtact cggacttcca aagaacttgc catagaaagt ttatctgcct ttaacaagat
    11641 atcttttggc atccattgat gaagatcgac atattgcatc ttattgactt catctttgat
    11701 tccttctgtt ttctcgtaat gaacagtcat gatttcatcc acagatggtg ctgtttgata
    11761 ttttggtgtt aacacttctg atgcttctga ttcttcgaac actcgagcat gaccgataaa
    11821 atattctttt gcaggagcaa gtgattcata taaatggatc cgtccatgga aatttttcat
    11881 ttttccgatt ttacgtccta aattatagcg tgttcctttt ggtaattttt tcaatccttg
    11941 agcaatcaca cggatgaatt ttgaattcgt atggaatcca tacgtttggt atcccgcaaa
    12001 cagttcatcc gccccttctc cagattggac tacccgcaca ctttgtgaag caagatttgc
    12061 taaaaagtac aaaggtacgc aagaaggatt tgaatcaggt tcatctaagt gatactggat
    12121 caaagggaat gctttgaacg acatgtctcc gtcgatcact gctgctgtat tatctaagtc
    12181 caatagttcg gttagttttc tggcttcaat cgcttcatta taggttttat catcaaaccc
    12241 gattgagaaa gaatgatctg gtttcaatac agaggttaca taactggaat caaccccgct
    12301 ggataagaaa gaaccaactt ctacatcact gaccgtatgt gcttcaatag aagcttgaac
    12361 ggtttcgtcg attttttcga tccattcttg tcgggaatgc gtttcttttg tttcaaaatc
    12421 tgcatcccaa tattgctgaa tgtctagttt tccatcttta tacgtataat aatggccttc
    12481 aggcaaacga tagacatctt tgaagaatgt ttcgccgttt aatggagaat attggaaagt
    12541 catataaggt ttcaacgctt ctttgttcaa ttctttgtta aaatcaggat gaggcagaaa
    12601 acttttgatt tctgaaccaa acataaatgt tccgttcatt tctgcataat aatatggttt
    12661 gatcccaaag tggtctcttg caccgaataa ctcattcttt tcattatccc aaatagcaaa
    12721 agcaaacatt ccgcggatct tttgcaataa ttctgttccc cattcttcgt aaccatgcaa
    12781 aaggacttct gtatctgcat gtgtttgaaa aacgtggcca gctgcaatca attcttctct
    12841 caaaggctgg taattataga tttcgccatt aaaaataatg atctttgtcc gatcttcatt
    12901 gtaaataggc tgagtaccgc cctctaagtc aataatactt aatcttctga atcctaaagc
    12961 aacatgttta tcgatatatt ctcctgaact attaggtcca cggtgaacga ttcgatccat
    13021 cattgtatta ataattgttt ttttgttgtc cttgtcgtta acaaaaccta caataccgca
    13081 catttaactt tcacatccta atataaaatt actgtttatt tcattcatat gcatcttatc
    13141 atagaataaa tagcggacga atattttctc tcttacaatt tcgtcacgtg aaaacaaaag
    13201 atgaaagttt ctgtttttac agaaaataaa gtcccaatat ctaatttacc atgttttctt
    13261 ttttgaggct accttattct cttcgtgtta aaaaaatatt agtggatgct atttccaaaa
    13321 aaaacagcaa aaaaagacac gacctaaaga tcgtgtctaa ttatgactgt atcagaaaat
    13381 atgataccaa acggatctat tcattaatta attgacatag tttatctgtt gctcttttat
    13441 gactggataa ctgaccaatt catgacgatc caagtcttct ttattcatat ttacagcagt
    13501 aatttgactg tcttcatacg tacgataata ataaatccct ttatcaacgt tacagcaaga
    13561 agaataaatc gtatattcgt attttccttc tccagtatcg catactcctt tttgttgctc
    13621 gactgagcct agtatatgaa aaaattgact gatactttca gattctgaat cacctgatgc
    13681 agcattcatt ttagtaaatg tggcttttac aaatcgagaa acagaagaga gatctccagg
    13741 taagcccaat cctcccatcc cacgactata aacattcaag ctgatctggt ttgaaaaatg
    13801 atttt
//
LOCUS       DAZKLD010000077        13758 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.077, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000077 DAZKLD010000000
VERSION     DAZKLD010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13758)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13758)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13758
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.077"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            134..376
                     /gene="yaaA"
                     /locus_tag="ACG8KR_001792"
     CDS             134..376
                     /gene="yaaA"
                     /locus_tag="ACG8KR_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HFQ0201159.1"
                     /translation="MKQQVILNTEFMTLGQMLKEVTVIGSGGQAKWYLAENTVLVDGE
                     PENRRGRKLYPGMMVEVPEVGTFFMVKKGNDDNEAE"
     gene            363..1487
                     /gene="recF"
                     /locus_tag="ACG8KR_001793"
     CDS             363..1487
                     /gene="recF"
                     /locus_tag="ACG8KR_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HFQ0201160.1"
                     /translation="MRLNKLYLKNYRNYEELNIEFSKNLVIFLGENAQGKTNLLESIY
                     VLAMTRSHRTSSEQELIGWTDDQAMIQGEITKGSSTLPLEILLSKKGRKTKINHIEQK
                     KLSTYVGQMNVILFAPEDLSLVKGSPQIRRKFLDMELGQINPIYLYDLVQYQAILKQR
                     NQYLKQLNEKKQTDLLYLDILSEQLAAFGSKVLKARAQFVKRLEFWANSLHQQITHQK
                     EQLEIEYLTAVDSLETHTQEQIQEQFLALLNQNKKKDLFRGTTTVGPHRDDLSFFINQ
                     KNVQTYGSQGQQRTTALSVKLAEIDLIKEETGEYPILLLDDVMSELDDNRQLHLLETI
                     EGKVQTFLTTTTLDHVKNKMTVEPEIFYVQQGKLKGEETE"
     gene            1484..3430
                     /gene="gyrB"
                     /locus_tag="ACG8KR_001794"
     CDS             1484..3430
                     /gene="gyrB"
                     /locus_tag="ACG8KR_001794"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HFQ0201161.1"
                     /translation="MTEERSLVERAKEYDASQIQVLEGLEAVRKRPGMYIGSTSSEGL
                     HHLVWEIVDNSIDEVLAGFATKIHVIIEKDNSITVIDDGRGIPVDIQAKTGRPAVETV
                     FTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKEGNVYYQEYRRGAVVD
                     DLKIIEQTDRHGTTVHFTPDPEIFTETTEFDFSKLATRIRELAFLNRGMRISIEDKRE
                     EEPVINEYHYDGGIKSYVEYLNANKTVIFPEPVYLEGEQQDIAVEVSMQYTDGYHSNI
                     MSFANNIHTYEGGTHESGFKTALTRVINDYARKQKLMKENDDNLTGEDVREGLTAVIS
                     IKHPDPQFEGQTKTKLGNSEVRTVTDRLFSEHFMKFLLENPSVGRQIVEKGLLASRAR
                     LAAKRAREVTRRKGALEISNLPGKLADCSSNDPEKCELFIVEGDSAGGSAKQGRNREF
                     QAILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGEDFDVSKARYHKLVIMTDA
                     DVDGAHIRTLLLTLFYRYMRPIVEAGYVYIAQPPLYGVKQGKNITYVQPGKNAEEELK
                     QVVASLPASPKPSVQRYKGLGEMDDHQLWETTMDPSNRMMARVSVDDAIAADQIFEML
                     MGDRVEPRRAFIEENAHYVKNLDI"
     gene            3450..5921
                     /gene="gyrA"
                     /locus_tag="ACG8KR_001795"
     CDS             3450..5921
                     /gene="gyrA"
                     /locus_tag="ACG8KR_001795"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HFQ0201162.1"
                     /translation="MSEEIRENIHDVNLTSEMKDSFIDYAMSVIVARALPDVRDGLKP
                     VHRRILYGMNELGVTPDKAHKKSARIVGDVMGKYHPHGDIAIYESMVRMAQPFSYRYM
                     LVDGHGNFGSVDGDGAAAMRYTEARMSKIATEMLRDINKNTVDFQSNYDDTEKEPVVL
                     PARFPNLLVNGTTGIAVGMATNIPPHNLSEVVAAIDLLMDNPDVTTNELMEVLPGPDF
                     PTGGLVMGKSGIRRAYETGKGSITVRAKVEITEMPNGKERIIVTELPYMVNKAKLIER
                     ISELHRDKRIEGITDLRDESSREGMRIVIDIRRDASASVILNNLYKLTSLQNSFGFNM
                     LAIEKGVPKVLSLKQILENYIEHQREVITRRTVFEKEKAEARAHILEGLRIALDHIDE
                     IIAIIRGSKSDDEAKATMIERFDLSDRQSQAILDMRLRRLTGLEREKIENEYQELLKL
                     IEDLTDILARPERVTEIIKNELADLNQRFGDKRRTELLVGEVLSLEDEDLIEEEEIVI
                     TLTNNGYIKRLANNEFRAQRRGGRGVQGMGIHNDDFVKNLVSCSTHDTLLFFTNNGKV
                     YRAKGYEIPEYGRTAKGIPIINLLGIDSSESIQAIIAVTGEAEEGHYLFFTTRQGTVK
                     RTSVTAFANIRSNGLIAIGLKEDDELVNVLLTDGQSNIIIGTHNGYSVTFKEEAVRDM
                     GRTASGVRGIRLREEDYVVGAALMKENQEVLVITEKGYGKRTKVSEYPVKGRGGKGIK
                     TANITEKNGPLAGLTTVSGDEDIMLITDKGVIIRFNVSTVSQTGRSTLGVRLMKMEAD
                     TKVVTMAAVEPEAAEQTVEEQGSEE"
     gene            6157..6456
                     /gene="rpsF"
                     /locus_tag="ACG8KR_001796"
     CDS             6157..6456
                     /gene="rpsF"
                     /locus_tag="ACG8KR_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005226363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HFQ0201163.1"
                     /translation="MENTKYEIMYIIRPNIDEEAKTALIERFDAILKDNGAEVIESKD
                     WEKRRFAYEMNGYREGIYHIVKVSSPSSANAVNEFDRLAKINDDIIRHMIVKEEA"
     gene            6506..7018
                     /gene="ssb"
                     /locus_tag="ACG8KR_001797"
     CDS             6506..7018
                     /gene="ssb"
                     /locus_tag="ACG8KR_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFQ0201164.1"
                     /translation="MINNVVLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQSGNR
                     EADFINCVIWRKSAETLANYARKGTLLGVTGRIQTRSYDNQQGQRVYVTEVVADNFQL
                     LESRSASENRQQSGGFQSSGQSAGGFGGNNNSNQTSQSSNGMPDFDRDTSDPFGSSST
                     IDISDDDLPF"
     gene            7044..7280
                     /gene="rpsR"
                     /locus_tag="ACG8KR_001798"
     CDS             7044..7280
                     /gene="rpsR"
                     /locus_tag="ACG8KR_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HFQ0201165.1"
                     /translation="MAQQRRGGRKRRKVDYIAANHIEYIDYKDIELLKRFISERGKIL
                     PRRVTGTGAKNQRKITVAIKRARIMGLLPFVSED"
     gene            7423..9396
                     /locus_tag="ACG8KR_001799"
     CDS             7423..9396
                     /locus_tag="ACG8KR_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HFQ0201166.1"
                     /translation="MENKNNHLSPKPLFLILFILIEVLVVLILPFRFVSASVILLMNL
                     WGVRYWLKMKRWLILNKREKIQQASTVAETNLAYVSEVMPVGVIAYQPSSQKIEWLNP
                     LALKIKDQSGLTEEELLDTFFTAKEKKKNQVYLADKTFQFDMDTVKGVVYFNDITQNQ
                     LLKLHQTNSQPVIGILSIDNYADAIDKLDDKEISYLNSFVTTLVSDWMNEYHVFFKRI
                     NAERFFFVAQMIDLKKMMDKKFDLLDRFRAEAEKQSSPITLSMGIAYGSRTLDEIGGE
                     AQNNLDVALVRGGDQVVVKEVKEGAKPVYYGGKSAKNVKRTRVRSRAMSTALRRIFDE
                     TGDVFIMGHKFPDMDALGSAFGVSCLARFNQKKAYIVINENEIIPDVERCLEEIHKHP
                     ELEMQLISPERAIELKKEEDLLVMVDYHKPSLSISQGLYEQFDKIVIIDHHRRGEEFP
                     SKPLLTYIESSASSASELVSELIQYQSSKTNRLDRFEATLLLAGMVVDTNSFNVRTTA
                     RTFDVASYLRSCGADASLVQYLLSSDLATYLEISNLIAGSDYVTSDIVVAAGTDEKEY
                     NSVIAAKTADTLLSMININAAFVITKRTDGLIGISARSTGTINVQIIMESLGGGGHFT
                     NAATQLENTTVEETKQKLFAAIKENMNQIYDKE"
     gene            9402..9854
                     /gene="rplI"
                     /locus_tag="ACG8KR_001800"
     CDS             9402..9854
                     /gene="rplI"
                     /locus_tag="ACG8KR_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HFQ0201167.1"
                     /translation="MKVIFLQDVKGKGKKGDIKEVPAGYAQNYLIKNNLAKEATKSSI
                     SQLAGQKKAQEKHEAEILEEAKKLKEFLEKEDTVVELKAKAGEDSRLFGSIPSKQIAE
                     GLNKQYKIKLDKRKIELENPIRSLGYTNVPVKLHHEVTATIKVHVVQE"
     gene            10128..11495
                     /gene="dnaB"
                     /locus_tag="ACG8KR_001801"
     CDS             10128..11495
                     /gene="dnaB"
                     /locus_tag="ACG8KR_001801"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HFQ0201168.1"
                     /translation="MNEVWQDRIPPQNIEAEQAVLGSVFLDAETIIDAMEYIEPKDFY
                     RRGHQIIFETMIELNDRNEAIDVVTVKDRLEQANLLEDAGGLSYLSDLALAVPTAANI
                     VYYAKIVEEKSLLRTLIQTATGIVTKGFEQGEEVQTILDDAERSILEVSEKRNRSGFL
                     SIAEVLNSTIENIDRLAQNNEEITGLPTGYQGLDKMTAGFQPEELIILAARPAVGKTA
                     FALNIAQNVGTKTDRSVAIFSLEMGAESLVNRMLCAEGSIEASHLRTGQLSEEEWSNL
                     IVAMGSLSRANIFIDDTPGIKISEIRAKCRKLAQEKGNLGLILIDYLQLIEGNGKENR
                     QQEVSDISRQLKKLAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYEREGGDDDDGNEPKNDNVIEVIIEKNRSGARGTVELLFIKEYNKFSSLS
                     PREEF"
     gene            12275..13567
                     /locus_tag="ACG8KR_001802"
     CDS             12275..13567
                     /locus_tag="ACG8KR_001802"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HFQ0201169.1"
                     /translation="MSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGDNAGHTIK
                     FNGVTYKLHLIPSGIFYKDKISVIGNGVVVNPKSLVKELAYLHEKGVETSNLRISDRA
                     HVILPYHIKLDQLQEDAKGENKIGTTIKGIGPAYMDKAARVGIRIADLLDKEIFEERL
                     RLNLDDKNRQFVKMFDSEPLDFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDETGEQIREVGREYGTTTGRPRRVGWFDSVVMRHSKRVSGITNLSLNSIDVL
                     SGLETVKICTAYELDGELIYHYPASLKELNRCKPIYEELPGWSEDITGCKTLAELPEN
                     ARNYVRRISELVGVRISTFSVGPDRTQTNILESVWAQI"
BASE COUNT         4585 a         2482 c         3021 g         3670 t
ORIGIN      
        1 gtttattttt gtttaaatca ccttttttag attaaatcgt cttatatgga aaaatatatg
       61 gataaatttg ttttattgcg aaaaaaaagg tataatagag tatactttta ataatgaaaa
      121 tgagggatgg gcattgaaac aacaagtcat tctaaatacg gaattcatga cgttgggcca
      181 aatgttgaaa gaagtcacag ttatcggtag tggcggacaa gcaaaatggt atttggcaga
      241 aaacactgtg ttagtcgatg gcgaaccaga aaatcgtcgt ggacgcaaac tttatcccgg
      301 catgatggtt gaagttccag aagtaggaac tttttttatg gtaaaaaaag gaaacgacga
      361 taatgaggct gaataagctg tatttaaaaa attatcgaaa ttacgaagag ctgaacatcg
      421 aattttccaa gaacctcgtc atctttttag gagaaaatgc gcaaggaaaa acgaatcttt
      481 tggaaagtat ctacgtatta gctatgacgc gaagtcatcg gacaagtagt gagcaggaat
      541 tgattggctg gactgacgat caggcgatga ttcaaggaga gatcacaaaa ggttcatcca
      601 ctcttccatt agaaatctta ttatctaaaa aaggccgaaa aaccaagatc aaccatatcg
      661 aacagaaaaa gttaagtact tacgtgggac aaatgaatgt cattttattt gcaccagaag
      721 atctttcttt agtaaaaggc agcccgcaga tcaggcggaa gtttttagat atggaattag
      781 gccagatcaa tccaatctat ctttatgatc ttgtccaata ccaagcaatt ttgaaacaaa
      841 gaaaccagta cttgaagcaa ttgaatgaaa aaaaacaaac tgatttgctt tatttggata
      901 ttttgtccga acaactagca gcgtttggca gcaaagtatt gaaagcaaga gcacagttcg
      961 tcaagcgatt ggagttttgg gctaattctt tgcatcagca aatcacccat caaaaagagc
     1021 agctggaaat cgagtatctc accgcagtcg attctttaga aacacatacg caagagcaaa
     1081 tccaagaaca atttctcgca cttttgaacc aaaataaaaa gaaagacctt tttcggggaa
     1141 caacgacagt tggtcctcat cgtgacgatc tttccttttt tattaatcag aaaaatgtgc
     1201 agacttatgg atcgcaagga cagcaaagaa caactgcact aagtgtcaaa ttagcagaaa
     1261 tcgatttgat taaagaagag acaggggaat atccaatcct tttgttagat gatgtgatga
     1321 gcgagctgga tgataatcgt caactccatc tgctagaaac aatcgaagga aaagttcaaa
     1381 cgttcttaac aacaacgaca cttgaccatg taaaaaataa aatgactgtt gaacctgaga
     1441 ttttttatgt tcaacaaggg aaactaaaag gagaagaaac tgaatgacag aagaaagaag
     1501 tttagtagaa cgcgctaaag agtatgatgc cagtcagatt caagtacttg aaggacttga
     1561 agccgttcgt aaacgacctg gcatgtatat cggttcgact agttctgagg gtctgcatca
     1621 ccttgtttgg gaaattgtcg ataattcgat cgatgaagta ttagcaggtt ttgcaacgaa
     1681 aatccatgtg attattgaaa aagacaacag tatcaccgtt attgacgacg gtcgggggat
     1741 acctgtagat atccaggcaa aaactggacg tcctgcagtt gaaacggtct ttactgtttt
     1801 acatgctgga ggaaaattcg gcggtggtgg gtacaaagtt tctggtggac ttcacggagt
     1861 aggatcttct gttgtaaacg cgctttctac acagcttgat gtcaaagtat acaaagaagg
     1921 aaatgtgtac tatcaagaat atcgtcgtgg agctgtagtg gacgacttaa agattatcga
     1981 acaaacggat cgccatggaa caacggttca ctttacgcca gaccctgaaa tttttacaga
     2041 gacaactgag tttgattttt caaaattggc tacccggatc cgtgaacttg ctttcttgaa
     2101 ccgcgggatg cgtatatcga ttgaagacaa acgagaagaa gaaccagtca tcaatgaata
     2161 ccattatgac ggcgggatca aaagttatgt cgaatacttg aatgcaaaca aaaccgtgat
     2221 ttttccagaa cctgtatatt tggaaggtga acagcaagat atcgcagttg aagtctctat
     2281 gcaatataca gacggctacc attccaatat catgagtttt gccaacaata tccatacgta
     2341 tgaaggtgga acacatgaat caggattcaa gactgcttta actcgtgtga taaatgatta
     2401 tgcccgaaaa caaaagctga tgaaagaaaa tgacgacaac ttgaccggtg aagacgtgcg
     2461 agaaggattg acagctgtta tttctatcaa gcatcctgac cctcaattcg aaggacaaac
     2521 aaaaacaaaa ttgggcaatt ccgaagtacg gacagtcact gatcggttat ttagcgaaca
     2581 tttcatgaaa ttcttgctgg aaaacccaag tgtgggccgg caaattgttg aaaaaggatt
     2641 actagcttcc agagctcgtc tagcagcgaa acgtgcgcgt gaagtaacga gacgtaaagg
     2701 tgctttagag attagtaatc tgccaggaaa attagcggac tgctctagca acgatcctga
     2761 aaaatgcgaa ttgttcatcg tcgaaggaga ttcggccggc ggatcagcta agcaaggtcg
     2821 taatcgagag tttcaagcca tcttgcctat tcgtgggaaa atcttgaatg ttgaaaaagc
     2881 aagcatggac aagatcttgg caaatgaaga gatccggtct ttatttactg ctatgggtac
     2941 agggtttgga gaagattttg atgtatcgaa agctcgctac cacaaattag ttatcatgac
     3001 tgatgccgat gtcgacggcg cgcatatccg tacattgctg ctgactcttt tttatcgtta
     3061 catgcgtcct attgttgaag ctggatacgt ctatattgct cagccacctt tatacggggt
     3121 aaaacaaggg aagaatatta cgtatgtaca accaggaaaa aatgcagaag aagagttgaa
     3181 acaagtcgtt gcttcacttc ctgcctcacc aaaaccaagc gttcagcgtt ataaaggact
     3241 tggagaaatg gatgatcatc aactatggga aacaactatg gacccaagca atcggatgat
     3301 ggcccgcgtc agtgtggacg atgctattgc agcagatcag atatttgaaa tgttgatggg
     3361 ggatcgagta gaaccccgtc gtgcattcat cgaggaaaat gcacactatg tgaagaactt
     3421 agatatttaa tcatgaagga ggaaaaagaa tgagtgaaga aatcagagaa aacatccatg
     3481 atgtcaatct aaccagcgaa atgaaagact ccttcatcga ttatgcgatg agtgttattg
     3541 tcgcacgtgc acttcctgat gtcagagatg gtctgaaacc cgttcatcgc cgtattttat
     3601 acggcatgaa cgaattaggt gtaacaccgg ataaagccca taaaaaatct gccagaatcg
     3661 tcggagatgt catgggtaaa taccatcctc atggagacat tgcgatctat gagtcaatgg
     3721 tacggatggc acagccattc agctaccgtt atatgctagt ggatggtcat ggaaactttg
     3781 gttctgttga cggtgacggt gccgccgcta tgcgttatac cgaagcacgg atgagcaaaa
     3841 tcgctacaga aatgctgcgt gatatcaata agaacactgt tgatttccaa agcaactatg
     3901 atgatacaga aaaagaacca gttgtactac ctgctcgctt cccgaatctt ttagtgaacg
     3961 gaacaacggg gatcgccgta ggtatggcca cgaatatccc acctcataac ttgtctgagg
     4021 tagtagcggc tatcgatttg ctgatggata atcctgatgt gacgaccaat gaattgatgg
     4081 aagtattacc tggaccagat tttccaacag gtggtttggt tatggggaaa tcaggaatcc
     4141 gaagagcgta tgaaacaggt aagggttcta ttaccgtacg tgcaaaagtt gagattactg
     4201 agatgccaaa tggtaaagaa cggatcatcg tgacagaatt gccttatatg gtcaataaag
     4261 caaaattgat cgaacggatt tctgaactgc acagagacaa gcgtatcgaa gggatcacag
     4321 atttgcggga cgaatcttcc cgtgaaggta tgcggatcgt cattgacatc cgtcgtgacg
     4381 ccagcgcatc cgttatcttg aataacttat ataaattgac atccttgcaa aattcatttg
     4441 gattcaatat gttggcgatc gaaaaaggtg tgccgaaagt tttgagcttg aaacaaatcc
     4501 ttgaaaacta tatcgaacat caacgcgaag tcatcacacg acggacggtc tttgaaaaag
     4561 aaaaagcaga agcaagagca cacatcttag aaggtttgcg tattgctttg gatcatatcg
     4621 atgaaatcat tgcgattatc cgcgggtcta aatcagatga cgaggcaaaa gcaacaatga
     4681 tcgaacggtt cgatttatcc gatcgccagt ctcaagctat tttagatatg cgtttacgcc
     4741 gattgaccgg tttggaacgg gaaaaaatcg aaaatgaata ccaagaactc ttaaaactga
     4801 ttgaagattt aactgacatt ctggcaagac cagaacgtgt tacagaaatc atcaagaatg
     4861 aattagcaga cttgaatcaa cgatttggtg ataaacgacg cacagaatta ctagtcggtg
     4921 aggtcttaag tctggaagat gaagatttaa tcgaagaaga agaaatcgtt attacattga
     4981 caaacaatgg ttacatcaaa cgtttggcaa ataatgaatt cagagctcaa cgaaggggtg
     5041 gacgtggtgt ccaaggaatg ggtatccaca atgacgactt cgtcaaaaat ctggtttctt
     5101 gttctaccca tgatacattg ttattcttta cgaataacgg aaaagtgtat cgggcaaaag
     5161 gatacgaaat tccagaatac gggcgtacag ccaaaggaat cccaatcatc aatttactag
     5221 gtatcgattc gagtgaaagc atccaggcaa tcattgcggt gacgggagaa gcagaagaag
     5281 gccactatct cttctttacg acgcgtcaag gtacagtaaa acgtacttct gtcactgctt
     5341 ttgctaacat ccgaagtaat ggtctgatcg caattggttt gaaagaagac gatgagctcg
     5401 tcaatgtgct actgacagat ggtcaatcta atatcattat tggaacacat aacggctact
     5461 ctgtcacttt caaagaagaa gcagtccgcg atatgggacg tacagcttcc ggggtcagag
     5521 ggatacgcct gcgtgaggaa gattatgtcg ttggggcagc cttaatgaag gaaaatcaag
     5581 aagtattagt gatcacagaa aaaggttacg gaaaacggac aaaagtatcg gaatatccag
     5641 tgaaaggccg cggaggtaaa gggatcaaaa cggcgaacat cactgaaaaa aatggtccac
     5701 tagctggatt gaccactgtt tctggagatg aagacatcat gctgattaca gataaaggtg
     5761 tcattatccg tttcaacgta tcgactgttt ctcaaactgg acgttcaaca ttaggcgtac
     5821 gtttgatgaa gatggaagct gatacgaaag ttgtcacgat ggcagcagtc gaaccagaag
     5881 ctgcagaaca aacagttgaa gaacaaggat cagaagaata accatcaaga ctaagtgtgt
     5941 aagctagcaa gaataaccga ttaaaaaatc tggttattcc tgctggcttt tttgattagt
     6001 tgaatttctc ttaaaatatt cgaaactgtt cattctgtat tgcttttgac caaaatagag
     6061 tgtataatta gagcttgtga gtagtctaat taattagata actctccttg ctcttaggaa
     6121 cctaagagcc gaaagtccac agggaggtga atatcaatgg aaaacacgaa atatgaaatc
     6181 atgtacatta ttcgtccaaa cattgatgaa gaagcaaaaa ctgctttaat cgaacgtttc
     6241 gatgcaatct tgaaagataa cggagcagaa gttatcgaat caaaagattg ggaaaaacgc
     6301 cgtttcgctt atgaaatgaa cggttaccgt gaaggtatct atcacatcgt taaagtatct
     6361 tctccatcat cagcaaatgc tgtcaatgaa tttgaccgtc ttgctaaaat caacgacgat
     6421 atcattcgtc acatgattgt taaagaagaa gcttaattgt tgtttcatgt gaaacaatag
     6481 aaaacgatga gaggagatga ctattttgat taataatgtt gtattagttg gacgtctgac
     6541 gaaagatcca gatttgcgtt acacagcaag tggtacggca gtcgcaacat ttactttggc
     6601 tgtgaatcgt aattttacaa accaaagcgg caatcgcgag gctgatttta tcaactgtgt
     6661 gatttggcgc aaatcagcag aaactttagc aaactatgct cgtaaaggaa cacttttagg
     6721 tgttactgga cgtatccaaa ctcgttctta tgataatcag caaggacaac gtgtatacgt
     6781 gacagaagta gtagctgaca acttccaatt gctggaaagt cgttctgcat ctgaaaatcg
     6841 ccaacaaagc ggcggattcc aaagttctgg tcaatcagct ggcggatttg gcggaaacaa
     6901 taactctaat caaacatctc aatcatccaa cggtatgcct gatttcgacc gtgatacttc
     6961 tgacccattc ggctcaagtt caacgatcga catttcagac gatgatttac cattctaaac
     7021 gaatttgata ggagggaaat agaatggcac aacaaagaag aggcggacgt aaacgtcgta
     7081 aagtagacta cattgctgct aaccatattg aatatatcga ttataaagat atcgaattac
     7141 taaaaagatt tatttcagaa cgcggtaaaa tcttgccacg tcgtgtaact ggtacaggcg
     7201 ctaaaaacca acgtaaaatt actgttgcaa tcaaacgcgc tcgtatcatg ggcttgctac
     7261 ctttcgttag cgaagactaa taaatcctag gaattgaatg actgatcctt ttgatcagct
     7321 attcaattcc tttttttctg taaaaataac ctctattcgt ttagtcgctc gaatgacgaa
     7381 agttatggta aaatcaaatt agtcatatat gggaggaaaa ttatggaaaa taaaaacaat
     7441 catttatccc cgaaacctct cttccttatc ctgtttatat taatcgaagt attggtagtc
     7501 ttaattttac catttcgttt tgttagcgct tcggtcattc tattgatgaa tttatggggt
     7561 gttcgctatt ggttgaaaat gaaacgttgg cttattttaa ataaacgtga aaaaatccag
     7621 caggcttcga ctgtagctga aaccaatcta gcttatgtct cagaagtcat gcctgtagga
     7681 gtgattgctt atcagccatc aagccaaaaa atcgaatggc tcaatccatt agcattgaaa
     7741 atcaaagatc aaagtggatt aacagaggaa gaattgctag atactttttt tactgcaaaa
     7801 gaaaaaaaga aaaaccaagt ctatctagca gataaaacat ttcagtttga tatggataca
     7861 gtaaagggtg ttgtttattt taatgatatt acccaaaacc agttgttaaa actccatcag
     7921 acgaacagtc agccggtgat tggtatttta tctattgata actatgcaga tgccattgat
     7981 aaattagatg acaaagagat ttcttatttg aactctttcg tcactacact agtttctgac
     8041 tggatgaatg aataccatgt ctttttcaaa agaatcaacg cagaacggtt tttcttcgta
     8101 gcacagatga tcgatctgaa aaagatgatg gataaaaaat ttgatttgct ggatcgcttt
     8161 cgagcagaag ctgaaaaaca gagctcgcct atcaccctta gtatggggat tgcttatggc
     8221 tcaagaaccc tcgatgaaat cggtggagaa gctcagaata atttagatgt tgctttagtc
     8281 cgtggaggcg accaagttgt cgtgaaagag gtaaaagaag gagcaaaacc tgtttattat
     8341 ggggggaaat ctgctaagaa tgtcaaacgc acgcgtgttc gttctcgagc gatgagtacc
     8401 gctttgcgtc ggatttttga tgaaacaggc gatgtgttta tcatggggca caagtttccg
     8461 gatatggatg ctttaggctc tgcttttggt gtcagttgtc tggcacgctt caaccagaaa
     8521 aaagcttaca tcgttatcaa tgagaacgag atcattccag atgtagaacg gtgtcttgaa
     8581 gaaatccata agcatcctga attagaaatg caactaatct ctccagaacg tgccatagag
     8641 ctgaaaaaag aagaggacct cctcgtgatg gtggattacc ataaaccttc tctatcgatc
     8701 tctcagggcc tatatgagca atttgataag attgtgatca ttgatcacca tcgtcgtggg
     8761 gaagagtttc caagtaaacc tctgctgact tatattgaat cttcagcttc ttcagcttca
     8821 gagctagttt ctgagctgat ccaataccaa agcagcaaaa caaatcgctt agatcgtttt
     8881 gaggccacat tattgcttgc agggatggtt gtagatacaa atagtttcaa tgtccgtaca
     8941 actgcacgaa cttttgacgt tgccagctat ttaaggtctt gtggagcaga cgcatcctta
     9001 gtacaatatt tattgagctc ggatttagcg acatacttgg agatcagcaa tttgattgct
     9061 ggaagcgact atgtgacttc tgatattgtt gtggcggcag ggacagacga aaaagagtat
     9121 aatagtgtta tagcagcaaa aacagcagat acattgcttt cgatgatcaa tatcaatgca
     9181 gctttcgtca ttacaaagcg gacagacggc ttgattggga tcagtgccag aagtaccggc
     9241 acgatcaacg tccaaatcat catggaatcc ttaggtggcg gaggacactt cacaaacgca
     9301 gctactcaac tggaaaatac gacagttgag gaaacaaaac agaagctatt tgctgcaatc
     9361 aaagaaaata tgaatcaaat atatgataag gagtaatcca aatgaaagta atttttttac
     9421 aagatgtaaa aggaaaaggt aaaaaagggg acatcaaaga agttccagca ggttatgcac
     9481 aaaattatct aatcaaaaat aacttggcaa aagaagcaac gaaaagcagt atcagtcagt
     9541 tagcaggaca aaagaaagca caagaaaaac acgaagctga aatcttggaa gaagctaaaa
     9601 agttaaaaga attcttggaa aaagaagata ctgtagtcga attgaaggcg aaagcagggg
     9661 aagacagccg tttgtttggt tcgatccctt caaaacaaat tgctgaaggg ttaaacaaac
     9721 aatacaaaat caaattagat aaacgaaaaa tcgaattaga aaatccgatc cgttcactag
     9781 gctacacaaa cgtacctgtt aagcttcatc atgaggtgac ggctacgatc aaagttcatg
     9841 tcgtacaaga ataacaaatc ttgatagcga tgttcagctg gtaagtataa ccagaaggaa
     9901 aaaagacaaa ccgaaattta tctgagaaaa cagctgcagc aatgaaagca aaagctgcaa
     9961 atcgatttca gattgatttc ggtttctttt ttgggaacag caaaatagca taaagaaaac
    10021 gagtataata aaagagaact atttttttat tacactttcg gtacgaatga tgacttttct
    10081 ctcattttct tgtaaaatga agcaattgaa gacaaggagc gatacgtatg aatgaagtgt
    10141 ggcaggatcg aatcccaccg caaaatattg aagctgaaca agcggtttta ggctctgttt
    10201 ttcttgacgc agaaacgatc attgatgcca tggagtacat agagccaaaa gacttttatc
    10261 gtcgtggcca tcaaattatt tttgagacaa tgatcgagtt aaatgatcgt aatgaagcga
    10321 ttgacgtggt tactgtcaaa gaccggctgg aacaagccaa tttgttagaa gatgccggtg
    10381 gtttaagtta tctctcagac ttggcattag cagttccgac agcagcaaat atcgtctact
    10441 atgcgaaaat cgttgaagaa aaatcattgc tgcgtacatt gatccagaca gcaacaggaa
    10501 tcgttacgaa aggattcgag caaggagaag aagttcaaac aattttagat gatgccgaac
    10561 gcagtatctt ggaggtttcg gaaaaaagaa atcgaagcgg ctttttatct attgctgaag
    10621 tactgaactc cacgattgaa aatatcgatc gactagctca aaacaatgaa gaaatcacag
    10681 gtctaccaac tggctatcaa ggtctggata aaatgacagc aggtttccag cccgaagaac
    10741 taatcattct tgctgcacgt ccagcggtag gtaaaacagc gtttgcattg aatattgctc
    10801 aaaatgtagg aacaaaaaca gatcgttctg ttgcgatatt tagtttggaa atgggggcag
    10861 aatctcttgt aaatcggatg ctttgtgcag aaggttcgat tgaggccagc catttacgta
    10921 caggtcagct ttcggaagaa gaatggtcga atctgatcgt tgcgatgggg agtctttctc
    10981 gagcaaatat ttttatcgat gatacaccag ggatcaaaat ctctgaaatc cgtgcgaaat
    11041 gtcggaaact agcgcaagaa aaaggcaatc taggcttgat attgatcgat tatttgcagt
    11101 tgattgaagg taacggtaaa gagaaccgtc aacaagaagt atcagatatc tctcgccaac
    11161 tgaaaaaact ggcgaaagag ctgaaagtac ctgtcattgc tctttcacag ttatctcgtg
    11221 gagtagaaca gcgacaagac aaacgaccag tgctaagtga tattcgtgaa tccggatcaa
    11281 ttgaacaaga tgcggacatc gtagccttct tgtatcgaga tgattattat gaaagagaag
    11341 gcggagatga cgatgatgga aacgagccaa aaaatgacaa cgtcatcgaa gtcatcatcg
    11401 agaagaaccg aagcggtgca cgaggaacag ttgaactact gtttatcaaa gaatacaata
    11461 agttttcgtc gctttcgcca agagaagaat tttaaagaag aggttgtgag ccttgagcaa
    11521 tcccgagagc tctcgtaaga agatgcaagg agtaaccgca ggaacgacta gagtacttaa
    11581 tttaaacaga tctccaaagg gattgtgaca aaagtcttgt cacagtccct ttttccgaat
    11641 aaacggtgtt caatcagctg acccttggta ttagctcagc aaacgtcaaa atatgaggaa
    11701 ctattttgac gtttgtttct ctaatactca ggtcaaaacg ctgatcttac gacctctttt
    11761 ccttaaacgg tgttctcctg ccaactcctc ggtcacaagt cacaatatca tcaaacatga
    11821 gaagcagttt tttgaaattg ctccttgtga ctcagagtta aacggcaggc tcacaacctc
    11881 tttcttaaac ggtgttcaaa agcttgcatc tgcggtaata cgcccaatca aacgaaaaat
    11941 atgagaagct atttttgttt ggctgttctt attacttgat gcaggacagc tttttcacaa
    12001 cctctgattc acggtgttcc aaaagtagct tcttcgaaaa ataagtcgaa atttcacaaa
    12061 aatttgagaa gcaattttcg tacattcctt cttatttctt gaagctgacc acttctgtta
    12121 caacctcttt tttaataacc attctaaaaa gataaagttc gttttttaag cgtttttata
    12181 aaataaaaat aaaaatcatt cgattttatt tgaaatatgt atctattatt gatacaatag
    12241 ttcaggatta aataatatag aacgaggtgt tcacatgtca tctgtagtag tagtaggtac
    12301 gcaatggggc gatgaaggga aaggaaaaat cacagacttt ctcagtgaaa atgctgaagt
    12361 gattgcacgg taccaaggtg gcgacaatgc agggcacaca attaagttta acggtgtgac
    12421 ttataaactg catttgattc cttctgggat tttttataaa gacaagatca gtgtgatcgg
    12481 aaacggcgtt gtagtcaatc ctaaatcatt ggttaaagaa ttagcttatt tacatgaaaa
    12541 aggtgtagaa acaagtaatt tgcgtatttc tgaccgcgcg cacgtaatct tgccttatca
    12601 tatcaaattg gaccaattac aagaagatgc taagggcgaa aataagattg gtacaacgat
    12661 taaaggaatc ggacctgcct atatggataa agcagctcgt gtaggtatcc gtattgcaga
    12721 cttattagat aaagaaattt ttgaagaacg tcttcgtttg aatctagatg acaaaaaccg
    12781 tcaatttgta aaaatgtttg attcagaacc acttgatttt gacgatattt ttgaagaata
    12841 ttatgaatac ggacaacaaa tcaaacaata cgtcacagat acttccgtta tcttgaatga
    12901 tgctttggat gcaggcaaac gcgtgttgtt tgaaggcgca caaggtgtaa tgctggatat
    12961 cgaccaggga acttatcctt ttgttacttc ttctaaccca gtagccggtg gggtaacaat
    13021 cggaagcggt gtagggcctt caaaaatcaa taaagttgtc ggcgtctgca aagcctatac
    13081 gtcacgtgtc ggtgatggac catttccaac tgaattattt gatgaaacag gagaacaaat
    13141 ccgcgaagta ggacgagaat acggtacgac aactggtcgt ccgcgtcgtg taggctggtt
    13201 tgattctgta gttatgcgcc attctaaacg agtttcaggt attaccaacc tatctttaaa
    13261 ctcgatcgat gtattaagtg gattggaaac agtaaaaatt tgtacggctt atgaattaga
    13321 tggcgaacta atctaccact atccagcaag cttgaaagag ttgaaccgct gcaaaccaat
    13381 ctatgaagaa ttgccaggct ggtcagaaga cattacagga tgcaaaacac ttgcagaact
    13441 gccagaaaac gcacgtaact atgtacgtcg tatctcagag cttgttggtg tacgtatctc
    13501 aacattttct gtcggacctg atagaacaca aacaaacatc ttagaaagcg tctgggcgca
    13561 aatctaagga taaaaacgac gactgaatca ggacgttctt accagagcga gtcgaagtgt
    13621 ttatctatac gataataata acttgaagag gtagtgaaag agacgtttag ccttattgcc
    13681 gataggctgt ctcttgaaca ctgtttattc agaataaggg actgtgacaa aaaccgtgtc
    13741 acagtccctt tttccgaa
//
LOCUS       DAZKLD010000078        13705 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.078, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000078 DAZKLD010000000
VERSION     DAZKLD010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13705)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13705)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13705
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.078"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..901)
                     /locus_tag="ACG8KR_001803"
     CDS             complement(<1..901)
                     /locus_tag="ACG8KR_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HFQ0201170.1"
                     /translation="MRKVIIDCDPGIDDTLALSLAVKSPDIEVVAITVVCGNVPADIG
                     TQNVLKCLERCDRLDIPVYQGMEKPLKQPFISAQDTHGMDGLGETNFPMILRKQAEPL
                     HAVDFLADYFKEKTNTSVIALGPLTNIAFALKVNPNIGKHMDRFVSMGGTYKSHGNCS
                     PVAEYNYWCDPEAASYVFENLKQTIEMVGLDVTREIVLTPTILEYCCQMNPEEGEYLK
                     AITRFYFDFHWKQERILGCVINDPLAVAYFIEGAICEGFKSFTAVETQGISRGQTLVD
                     RYEFWQKPANSKIMTKVDTRLFSE"
     regulatory      complement(925..969)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            1184..2152
                     /locus_tag="ACG8KR_001804"
     CDS             1184..2152
                     /locus_tag="ACG8KR_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFQ0201171.1"
                     /translation="MVIFYFILILIIIGISYHFLSYFYAKKLVRAGVQRGYDWYEDTG
                     HRLMDPAAVTSVEKKRQKLEEECESFWEQGTPKTIKSYDGLKLAGQMFIHPNKQNKWV
                     ICVHDYRSTGKRDMSHIGKRYAEKGFNVLVPDLRAHGESEGEIIGMGWLDRLDLIAWI
                     QLILDEQPNASIILHGGSMGASTIMMASGEKLPSAVKGFILDSGYVSVYAEFRYMLSK
                     ITVFPKKMVMRYANHYAQKYAGYSLKQASATRQLGSNHLPLLVIHGERDHFVPTEAAY
                     TIQNATAGDKALLLVPEAEHLEASMKDPKTYWTVIFSFIEQRITMD"
     gene            2265..2543
                     /locus_tag="ACG8KR_001805"
     CDS             2265..2543
                     /locus_tag="ACG8KR_001805"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0201172.1"
                     /translation="MEIKEEKNRLALFNDEQQEIGEMTWSDAGPDIMIIDHTFVDPAY
                     RGQKLAEKLVYTGVELARRDGKKIIPLCPYAKKEFEKKPEYQDVLRQS"
     gene            2661..2897
                     /gene="secG"
                     /locus_tag="ACG8KR_001806"
     CDS             2661..2897
                     /gene="secG"
                     /locus_tag="ACG8KR_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015136 - sialic acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015739 - sialic acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HFQ0201173.1"
                     /translation="MYNLILGIVIVLSIVMVIAIMMQPSKQNSAASAFTGGADQLFGK
                     QKARGFEAVMQRSTAVMGAIWMILLFVLAYLSSK"
     gene            3000..3755
                     /locus_tag="ACG8KR_001807"
     CDS             3000..3755
                     /locus_tag="ACG8KR_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFQ0201174.1"
                     /translation="MRRVRQLPKPLYAKHGKRAVLLLHAYSGSPNDVRMLARFLEKAD
                     YTVYAPLFKGHGTMEPYDILQEKAESWWADTKKAIHFLQSEQFSDIAVLGLSMGGIFA
                     VRALEEESVIGGGFFCSPLSPVKTNVPENFEKYVRQVLKTAGKSEKEINEKAVAYRSL
                     AEQQLMDIQDQAAIVESRLSDIQQPIFLAQAGKDEMIDPNGVFETARKLSRQRVTLQW
                     YPESGHVITVGTARRELEKDVLEFLEKLPWNEE"
     gene            3758..6115
                     /gene="rnr"
                     /locus_tag="ACG8KR_001808"
     CDS             3758..6115
                     /gene="rnr"
                     /locus_tag="ACG8KR_001808"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HFQ0201175.1"
                     /translation="MTKETIKEKILFFMESSRKRSFSMEEIAEGLGFQKSDDFKLLVQ
                     TIAQMEREQSIVFNKKGKVKLPLKPILIEGTFRANERGFGFVTIDPEEDDIYIPKEAT
                     GFAMDGDTVAIDIMKRANTDMDRGAEGKVVEIRDRATTQLAGEFVAYSEEEIQETDLY
                     GVVIPKDRKLNQFKVYAAAEGVRPVDGSIVLIELTHYPEKGYATSLEGIIKQVIGHKN
                     DPGMDILSIVVANGIPTKFPDDVLSEADEVPDQISEKDITGRRDLRNQLIVTIDGEDA
                     KDLDDAVTVKKLDNGNYFLGVHIADVSYYVTQGSQLDREAYERGTSVYLTDRVIPMIP
                     QRLSNGICSLNPHVPRLTMSCEMEIDPNGQVVSHDIFPSVIQTTERMTYTAVNQILED
                     QDEETMQRYAQLVPMFQEMGELHRILETMRIKRGAISFEDREAKILVDAEGHPNDIQL
                     RSRGIGERLIESFMLAANETVAEHYNKLNLPFIYRIHEQPKEEKMQRFFDFASALGIL
                     VRGTKGTITPKDLQHVIEGVEDKPEAAVINTMLLRSMQQARYSEDNFGHYGLAADYYT
                     HFTSPIRRYPDLIVHRLIRSYSEDKSESNQSSWAAELPEIADHSSKMERRAVETEREV
                     DSMKKAEFMMDKVGEEFDGIISSVVKFGLFIELPNTVEGLIHINELKQDYFHFVENHL
                     ALVGERTGLTFKIGQKVRVKVVKADPDERAVDFELVSAEEVTALERPKARNGKFGKRE
                     GKQAGKRSEKNGKKNKRYSDSKQTFGKKKGKKPFYKDVKKKKKKK"
     gene            6137..6601
                     /gene="smpB"
                     /locus_tag="ACG8KR_001809"
     CDS             6137..6601
                     /gene="smpB"
                     /locus_tag="ACG8KR_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HFQ0201176.1"
                     /translation="MPKGEGKLIAQNKKARHDYSIIDTMEAGMVLQGTEIKSIRNSRI
                     NLKDGFIRVRNGEAFLHNVHISPYEQGNIFNHDPLRTRKLLLHKKQIVRLENELKNTG
                     ITVVPLKVYIRNGYAKVLIGLAKGKKSYDKREDLKRKDIDRQIDRTLKNFSR"
     gene            6903..7622
                     /gene="atpB"
                     /locus_tag="ACG8KR_001810"
     CDS             6903..7622
                     /gene="atpB"
                     /locus_tag="ACG8KR_001810"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HFQ0201177.1"
                     /translation="MDERSLTFHIGPIWFDGTVCIMVLLTCLIVFFLAYFFTRNLKLK
                     PTGKQNAIEWVIDFSRGIVTDNLPRKELSNFHLLAFTLFLFVFVANNIGLVTKIVLPN
                     ETTLWKSPTADPFVTLTLAFIMILLTHLFGVKKLGFKGYFVNSFLKPYSFMFPMKLIE
                     EFTNLLTLALRLYGNIYAGEVLLTLIAKMMTSLGWFSLPLAIPLEMVWIAFSLFIGSI
                     QAFVFVTLSMVYMSHKIEVEE"
     gene            7675..7890
                     /gene="atpE"
                     /locus_tag="ACG8KR_001811"
     CDS             7675..7890
                     /gene="atpE"
                     /locus_tag="ACG8KR_001811"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HFQ0201178.1"
                     /translation="MNYIAAAIAIMGAAIGAGYGNGQVISKTIESMARQPEMSGQFRT
                     TMFIGVALVEAVPILGVVIALILVFAV"
     gene            7981..8505
                     /gene="atpF"
                     /locus_tag="ACG8KR_001812"
     CDS             7981..8505
                     /gene="atpF"
                     /locus_tag="ACG8KR_001812"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HFQ0201179.1"
                     /translation="MLNQLAIAEVGNAMLGNIIVVSGSFLILLALLKHFAWGPISDIL
                     KKREDKIANDLDSAEQSRINSAKMEQEREQQLLASRSDAADIIKNAKESGELSRQNIL
                     KDAQEEAARLKSKAQADITVERDSALNSVKDDVAELSLQIAEKILNKELSPEMHESLI
                     NQYIEGLGSSNETR"
     gene            8492..9034
                     /locus_tag="ACG8KR_001813"
     CDS             8492..9034
                     /locus_tag="ACG8KR_001813"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HFQ0201180.1"
                     /translation="MKLDKYTVGRRYGKALFELAIDSNQAEEIYQDLVSLRQIYEQVP
                     GLGNMLSDVRLEPHEKREIMDKLVSGYDGIVKNFLEVVYEYNRMDDLLFMIEEYERRY
                     DEHQGLLLGSVTTAVPLSDEQRIQLEKNVAKTMDYQSVELKQIVDSSIIGGAIVEANH
                     RVIDGSIRTQLEKMRNQLNR"
     gene            9057..10613
                     /gene="atpA"
                     /locus_tag="ACG8KR_001814"
     CDS             9057..10613
                     /gene="atpA"
                     /locus_tag="ACG8KR_001814"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HFQ0201181.1"
                     /translation="MAIKAEEISALIKEQIENYQNVLSVEEIGTVTYVGDGIARAHGL
                     ENAMSGELLEFSNGSYGMAQNLESNDVGIIILGDFESIREGDKVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPIDGLGEIVTDKARPVEAMAPGVMQRKSVNEPMQTGLKAIDALVPIG
                     RGQRELVIGDRKTGKTSIAIDTIINQKGQDMICIYVAIGQKDSTVRTQVETLKKYGAM
                     DYTIVVNAGASQPAPLLYIAPYAGTAMGEEFMYNGKHVLIIFDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDDLGGGSMTALPFVETQAGDISAYIPTNV
                     ISITDGQIFLESDLFYAGTRPAVDAGLSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEILKQKLHAPLAVEKQVVILYALTHGFLDSIPV
                     DSILDFEHELFEYLDTNHSDIFETIRTTKDLPEEEALNSAIQEYKDMFLATKGNNSST
                     EDKLKSIQNA"
     gene            10627..11529
                     /locus_tag="ACG8KR_001815"
     CDS             10627..11529
                     /locus_tag="ACG8KR_001815"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HFQ0201182.1"
                     /translation="MGASLNEIKTRIASTKKTSQITRAMQMVSASKLTKSEASSQKFQ
                     VYATKVREIVTHLTATQLNDIASENPRGDINYNSMLLSRPVKKTGYIVITADGGLVGG
                     YNSSILKQTMSILEEDHQSPDDYVMIAIGGTGADFFKARGINLAYELRNLSDQPSFDE
                     VRKIVNMATTMYQNEVFDELYVCYNHHINSLTSQFRVEKMLPISDLDPEEATTFEQEY
                     IFEPSKEEILSQLLPQYAESLIYGAIVDAKTAEHAAGMTAMKTATDNAANIIDELTVS
                     YNRARQGAITQEITEIVAGASALE"
     gene            11552..12958
                     /gene="atpD"
                     /locus_tag="ACG8KR_001816"
     CDS             11552..12958
                     /gene="atpD"
                     /locus_tag="ACG8KR_001816"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HFQ0201183.1"
                     /translation="MSSGKIVQVIGPVVDVEFSLDQSLPDINNALVVYKNDENKSKVV
                     LEAALELGDGVIRTIAMESTDGLQRGMEVIDTGKAISVPVGKETLGRVFNVLGDTIDL
                     ETPFPEDAERSEIHKKAPAFDELSTSTEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYYEMKDSGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSITSIQAIYVPADDYTDPAPATAFAHLDATTNLERKLTE
                     QGIYPAVDPLASSSSALAPEIVGEEHYKVATEVQHVLQRYRELQDIIAILGMDELSDQ
                     EKVLVSRARRVQFFLSQNFNVAEQFTGLPGSYVPVEETVKGFREILEGKYDDLPEEAF
                     RSVGRIEDVVEKAKTLGY"
     gene            12972..13394
                     /locus_tag="ACG8KR_001817"
     CDS             12972..13394
                     /locus_tag="ACG8KR_001817"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HFQ0201184.1"
                     /translation="MDQYLTVNVVTPAGLVYDHHAAIVVARTTAGELGILPKHAPIIV
                     PLTIDEVRVKRTDSDTHVDWIAVNGGIMEVRDNVVSIVADSAERERDIDVSRAERAKQ
                     RAERQIEEAKAKQDTNELKRATVALHRAINRIKVSKHS"
BASE COUNT         4529 a         2453 c         2988 g         3735 t
ORIGIN      
        1 tttcggaaaa aagacgagta tctacttttg tcataatttt actgttagca ggtttctgcc
       61 aaaattcata acgatcaacc aatgtttgtc cacgacttat tccctgagtt tccacagcgg
      121 tgaagctttt gaaaccttca cagatggccc cctcgatgaa ataagcaaca gctaacggat
      181 cattgatgac acatcctaaa attctttctt gtttccagtg gaagtcaaaa taaaaacgag
      241 tgatcgcttt taaatactct ccctcttcag gattcatttg acagcagtat tcgaggatag
      301 tcggtgtcaa gacgatttct cttgtcacat ctaacccaac catttctatc gtttgtttga
      361 gattctcgaa tacataagaa gctgcttcag gatcacacca gtaattgtat tcagcaactg
      421 gcgaacagtt accatggctt ttgtaagttc cacccatcga gacaaaacga tccatatgtt
      481 tcccaatatt tgggttgact ttcaaagcaa aagcgatatt ggtcaaagga cccaaagcaa
      541 tgactgatgt atttgtcttt tctttgaaat aatcagccaa aaaatcaacg gcatggagag
      601 gttctgcctg ttttctcaag atcatcggga aatttgtttc ccctaaacca tccataccat
      661 gagtgtcttg tgcactgatg aaaggttgct tcaaaggttt ttccattcct tgataaacag
      721 gaatgtctaa acgatcacag cgttccaggc atttcagcac attctgcgta cctatatctg
      781 cagggacatt cccacaaact acggtaatag caactacttc gatatcaggg gatttgacag
      841 ctaatgataa agctaaagtg tcgtcgatcc ctggatcgca atcaataatt acttttcgca
      901 ttatttccaa tctcctttct ttagagatac ttagtttttt attctgggaa gtttccgaac
      961 cagtccaaga cgagcgtctt gattataatg cacgaaatta gtatactggc ttttattgcg
     1021 aatgtaaatg ctctcttaca agtaaatcta ttgaggagaa ctttaagtaa atgatacaat
     1081 gaaaaagaaa tggaatttac gtgtgccgaa aataaagaat cctatggatc ggtacattgg
     1141 ataatagata gattatagtt tggcagaaag cggcgggtgt acaatggtta ttttttattt
     1201 cattcttatt ctgataatta tagggatcag ttaccatttt ctttcttatt tttatgcaaa
     1261 gaaattagta agagcgggag tacagagagg atatgactgg tatgaagaca ctggtcatcg
     1321 tttgatggac cctgctgcag tcacgtcagt agaaaaaaaa cgacaaaaat tagaagaaga
     1381 gtgtgaaagc ttttgggagc agggaacacc caaaacaatc aaaagttatg acgggttgaa
     1441 actagctggt cagatgttca tccatccaaa taagcagaat aaatgggtca tttgcgtaca
     1501 tgattaccga agcacaggga aaagagacat gtctcatata ggtaaaagat atgcagagaa
     1561 aggattcaac gttcttgtac ctgatttacg tgcgcacgga gaaagtgaag gagaaatcat
     1621 cggaatggga tggctagatc ggctagattt aattgcctgg atccaactca tcttagatga
     1681 acagccgaat gcgtccatta ttttacacgg cggatcaatg ggagcttcta cgatcatgat
     1741 ggcaagtggt gaaaaacttc catcagcggt caaaggattt attttagaca gcggatatgt
     1801 ttctgtttat gcagaattcc gatatatgtt aagtaaaatc actgtctttc ctaaaaaaat
     1861 ggtcatgaga tatgcgaatc attatgccca gaaatacgcc ggttactcct taaagcaagc
     1921 atcagctacg agacagcttg gcagcaacca tctgccttta ttagtcatcc atggtgaaag
     1981 agatcatttt gtccctacag aagcagcgta cacgattcaa aatgctacgg caggggataa
     2041 agcactgctg cttgttccag aagcagaaca cttggaagca tcgatgaaag atccgaaaac
     2101 ctattggaca gtgatttttt catttattga acaaagaata acgatggatt aattttctct
     2161 tcttttttgt aagtaattcg aggataaata attgtttata gatctggaga ctgctaaaat
     2221 aaggtaggaa taaaaaaata tcagttattt gaggagtgcg gcatatggaa atcaaagaag
     2281 aaaaaaatcg tcttgcttta ttcaatgatg agcaacaaga gattggagaa atgacatggt
     2341 cagatgcggg cccagacatc atgatcattg accatacatt tgtagatcca gcatatcgtg
     2401 gtcaaaagtt agctgaaaag ctagtgtata ctggagtaga attagcaaga cgagacggga
     2461 aaaagatcat tcctttatgc ccttatgcaa aaaaagagtt tgaaaagaaa ccagaatatc
     2521 aagacgtctt acgtcagtca taagaataac agctgaaagt tttgaacttt cagctgttat
     2581 tccatttata tagagttgtc acggactttt atctatgata aagttaaaga gacgatatct
     2641 tttgggaagg aggctgcgat atgtataatt taattttggg aatcgtgatc gttttatcga
     2701 ttgttatggt cattgcgatt atgatgcagc caagcaagca aaatagtgca gcgagtgcat
     2761 ttaccggcgg tgcggatcaa ctattcggca aacaaaaagc tcgcggcttt gaagctgtca
     2821 tgcagcgatc aactgcagtt atgggagcta tctggatgat cttattattt gtcctagcat
     2881 atttatcttc gaagtagttg aataagcctc tcgcaaaacg gcgggcggct ttttttattg
     2941 caaagagaac gcttaagaag aagttatgaa agaaactatg gtaaaatgag gcgaaagagg
     3001 tgagacgagt gagacaatta ccaaaacccc tttatgcaaa acatggaaag cgagcggttt
     3061 tgctgcttca cgcttactca ggaagtccaa acgatgttcg aatgctcgca cgatttttgg
     3121 aaaaagcaga ttatacagtt tatgcgccgc tttttaaagg acacggaaca atggagccat
     3181 atgatatctt gcaagaaaaa gcagaaagct ggtgggcaga cactaaaaaa gccatccatt
     3241 ttttacagtc tgagcagttt tctgatattg ccgtattagg attatcaatg ggcggcatct
     3301 ttgctgtacg agcactagaa gaagaatcag tgattggcgg cggttttttc tgttcgcctt
     3361 tgtctcctgt aaaaacaaac gtgcctgaaa actttgaaaa atacgtacgt caagtgctga
     3421 agacagcagg taaatcagag aaagaaatca atgaaaaagc agttgcttac cgctcgttag
     3481 ctgaacaaca attaatggat atacaagatc aggcagcaat cgtagaaagt aggttgtcag
     3541 atatccaaca gccgatattt ttagctcaag ctgggaaaga tgaaatgatc gaccctaatg
     3601 gcgtatttga aactgcacgc aagttgagcc gtcagcgagt gacacttcaa tggtatcctg
     3661 aaagtggtca tgtgattaca gttggtacag caagacgcga attagaaaaa gacgtcttgg
     3721 aatttttaga aaaattacct tggaatgagg aataaatatg acaaaagaaa cgataaaaga
     3781 gaaaattctc tttttcatgg aaagcagtcg caaacgcagt ttttctatgg aagaaatcgc
     3841 tgaaggttta gggttccaaa aaagcgatga ttttaaatta ctagtacaaa cgattgctca
     3901 aatggaaaga gaacagagta tagttttcaa taaaaaagga aaagtgaaac ttcctttaaa
     3961 accaatcttg atcgaaggaa catttcgagc aaatgaacga ggatttggat ttgtaacgat
     4021 tgatcccgaa gaagatgaca tctatatccc aaaagaagcg actggttttg caatggatgg
     4081 agacaccgta gcaatcgata tcatgaagcg agcaaataca gatatggatc gcggagcaga
     4141 ggggaaagta gtcgagattc gagaccgggc gactactcag ttagcagggg aattcgttgc
     4201 ttattcagaa gaagaaatac aagaaacaga tctttacggc gttgttatcc cgaaagatag
     4261 aaagctcaac caatttaaag tatatgcagc agcagaggga gttcgtccag ttgacggaag
     4321 tattgtgctg atcgaactga cccattaccc tgaaaaaggg tatgcgacta gtttagaagg
     4381 gataatcaag caagtgattg gtcataagaa tgaccctggc atggatatcc tctcaattgt
     4441 cgtggctaat ggtatcccta cgaaatttcc tgatgatgta ttgtctgaag cagatgaagt
     4501 tccagatcaa atcagcgaaa aggatataac tggacgtaga gatttaagaa atcagctcat
     4561 tgttacgatt gatggagaag atgcaaaaga tctagatgat gcagttacgg ttaaaaaatt
     4621 agataacggt aattattttc ttggagtaca cattgcagat gtttcttact atgtgacaca
     4681 aggcagtcaa ttggacagag aagcatatga aagagggaca agtgtatatt tgactgatcg
     4741 agtgattccg atgatcccgc aacgtttatc gaatggtatc tgttcactga atccccatgt
     4801 tccaaggctg accatgagtt gcgaaatgga gattgatcca aatggacaag tagtctctca
     4861 tgatattttc ccaagtgtga tccaaacaac agaacggatg acctacacag ctgtcaatca
     4921 aatcttagaa gatcaagacg aagaaacgat gcaacgctat gctcagctcg tacctatgtt
     4981 ccaagaaatg ggtgaattgc atcgaatcct tgaaacgatg cgaatcaaac gtggcgcgat
     5041 ttcttttgag gatcgcgaag cgaaaatcct tgttgatgct gaaggacacc caaatgacat
     5101 ccagctaaga agcagaggaa ttggtgaacg cttgatcgaa tctttcatgc ttgcggccaa
     5161 tgaaacggta gctgaacatt ataataaatt aaatctgccg tttatttacc gaatccatga
     5221 acagccaaaa gaagaaaaaa tgcagcgctt ctttgatttt gcttcagctt tgggcattct
     5281 ggtccgagga acgaaaggaa cgatcacacc aaaagattta cagcatgtga tcgaaggagt
     5341 agaagacaaa ccagaagctg cagttatcaa tactatgttg ctaagaagca tgcaacaagc
     5401 acgctattca gaagacaatt ttgggcatta tggcttagcg gcagattact acacacactt
     5461 tacatctcct attcgacgct atcccgatct aatcgtccat cgtctgatcc gttcttacag
     5521 tgaagataaa agtgaaagca atcaaagttc gtgggctgcg gaacttcctg aaattgcaga
     5581 tcatagttct aagatggaac gtcgtgcagt agaaacagaa cgtgaagtag attccatgaa
     5641 gaaagcagaa ttcatgatgg acaaagtggg agaagaattc gatgggatca tcagctctgt
     5701 tgtgaaattc ggcctgttta tcgaactgcc gaatacagta gaaggattga tccatatcaa
     5761 tgaattgaaa caggactact tccattttgt tgaaaatcat ttagctcttg taggtgaaag
     5821 aactggtctg acttttaaaa tcggacaaaa agtgcgcgta aaagtagtca aagctgatcc
     5881 agatgaacga gcagtggatt ttgaactcgt ttccgcagaa gaggtcactg cgttggaacg
     5941 tccaaaagca cgcaatggaa aatttggaaa acgagaagga aaacaagcag gcaaacgctc
     6001 agaaaaaaat ggcaagaaga acaaacgata tagtgatagt aagcaaacgt tcggtaagaa
     6061 aaaagggaaa aagccatttt acaaagatgt caaaaagaag aaaaagaaaa aataaaatga
     6121 gttggaggaa acagctatgc caaaaggcga gggaaaacta attgcacaaa acaagaaagc
     6181 tcgccatgat tattcgatca tcgacacgat ggaagcaggg atggtcttgc aaggaacgga
     6241 gatcaagtcg atacgaaaca gccggatcaa tctgaaagat ggatttattc gcgtccgcaa
     6301 cggggaagct ttcttgcata atgttcatat cagtccttat gaacaaggaa atatttttaa
     6361 tcatgatccg ttgcgcacga gaaagttatt attacacaag aaacaaatcg ttcgtcttga
     6421 aaacgaattg aaaaacactg gaatcactgt tgttccttta aaagtctata ttcgtaacgg
     6481 ctatgccaag gtattgattg gtctggcaaa agggaaaaaa tcgtatgata aacgggaaga
     6541 tttgaaacga aaagatatcg atcgacaaat tgatcgaaca ttaaaaaatt tctctagata
     6601 atttcattat cctttttcgc atattcggtg cttttgcttt tacttaatga taaaatttgt
     6661 tagcatttaa tctgataaaa catgattatc acaggttttt tcttagatca ggagcaccgt
     6721 gcatggggaa aaattcgctt cgtttttgac tgaaataaaa aagtgtctaa ttttcatgaa
     6781 aatgttttga attttctgtt tcatggtggt atgataccgt gtggttagaa accgattgga
     6841 aaaaaccaga tacgcacatg aattgatttt atgtgaattt cggggaaaag aggtgaaatg
     6901 gattggatga acgctcgcta acgttccaca ttggaccaat ttggtttgat ggtacagttt
     6961 gtataatggt gttactgacc tgtttgattg ttttcttctt agcttacttt tttacaagaa
     7021 atcttaagtt gaaaccaaca ggcaaacaaa atgcgataga atgggtcatt gattttagtc
     7081 gaggaatcgt aacagataat ctgcctagaa aagaattgag caatttccat ttgttggcat
     7141 ttacattgtt tttatttgtt tttgttgcaa acaacatcgg tttagtaaca aaaatcgttt
     7201 tacctaacga aacaacactt tggaaaagcc cgacagcaga cccgtttgtt actttaactt
     7261 tggcatttat aatgatctta cttactcact tattcggggt gaaaaagtta ggcttcaaag
     7321 gatatttcgt aaattcgttc ttgaaaccct atagttttat gtttccgatg aaactgattg
     7381 aagaatttac aaacttatta acacttgctt tacgtcttta tgggaatatt tatgcggggg
     7441 aagttttgct aaccttgatt gcaaaaatga tgacaagctt aggctggttc tcattaccat
     7501 tagcaattcc attagagatg gtctggatcg cgttctcctt gtttattggg agcatccaag
     7561 catttgtctt tgtcactttg tcaatggttt atatgagcca taagattgaa gtagaagaat
     7621 aatactcggt taaaaaacaa actactattt atctatttta ggaggaaaac aattatgaat
     7681 tatatcgcag cagcaatcgc aatcatggga gcagcaatcg gagccggtta tggtaacggt
     7741 caagttatct ctaaaacaat cgaatcaatg gctcgccaac ctgaaatgtc tggtcaattc
     7801 cgtacaacaa tgtttatcgg ggtagctcta gttgaagctg ttcctatctt aggggtagtt
     7861 atcgccttga ttctagtttt tgccgtttaa ttgaatcagt attaaggcag aaacaacatc
     7921 gtttgttact gcttttttac atggaaacta gaaatactcc aaagaaagga aggctcgcct
     7981 atgctgaatc aattggcaat tgcagaagtt ggcaatgcga tgttaggtaa tatcatcgtt
     8041 gtcagcggct catttttgat actactggct ctcttgaagc actttgcttg gggaccaatc
     8101 agcgatattt tgaaaaaacg tgaagacaag atcgccaatg atttagattc tgcagaacaa
     8161 tctcgcatca actcagcgaa aatggaacaa gaacgcgaac aacaattgtt agcctctcgt
     8221 tctgatgcag ctgatatcat caaaaatgcg aaagaaagtg gagaattaag ccgccaaaat
     8281 attttgaagg atgctcaaga agaagcagct cgtctaaaaa gcaaagccca agctgatatc
     8341 actgtagaac gtgattcagc gctgaactct gtaaaagacg acgttgcaga actctctctt
     8401 caaatcgcgg aaaaaatctt gaacaaagaa ttatctccag aaatgcatga atcattaatc
     8461 aatcaataca ttgaaggtct aggttcttca aatgaaacta gataaatata cagtaggaag
     8521 acgttacggg aaagctttat ttgaactggc gatcgattca aatcaagcag aagaaatcta
     8581 tcaagatctc gtaagtttac gtcaaatcta tgaacaagta cctggattag ggaatatgtt
     8641 aagtgatgtt cgtctagaac ctcatgaaaa acgagaaatc atggacaagc ttgtcagcgg
     8701 ttatgatggg atcgttaaaa actttttaga agttgtttat gagtataacc gaatggatga
     8761 ccttttgttc atgatcgaag aatatgaacg tcgatatgat gaacatcaag ggttactatt
     8821 gggaagtgtc acaacagcag tcccactttc agacgaacaa cgaatacaat tagaaaagaa
     8881 tgttgcaaaa acaatggact atcaatcagt tgaattaaaa caaatcgttg attcttctat
     8941 tattggtggc gcgattgttg aagccaacca tcgagtaatc gacggaagca ttcgcacgca
     9001 attagaaaaa atgcgtaatc aattaaacag atagtacaaa gaggtaggtg aatcgaatgg
     9061 ccatcaaagc agaagaaatt agtgccttga ttaaagaaca aattgaaaat tatcaaaacg
     9121 tgctttcagt cgaagaaatc ggtaccgtaa cttatgtagg tgacgggatc gctcgtgcac
     9181 atggtttaga aaacgcgatg agtggtgagt tgcttgaatt ttcaaacggc tcatacggaa
     9241 tggcgcaaaa cttagagtca aatgatgtag gtatcattat ccttggcgat ttcgagtcca
     9301 ttcgtgaagg agataaagta aaacgaacag gtaagatcat ggaagttcca gttggagagg
     9361 ccttgatcgg tcgggtagtc aatccgctag gtcaaccaat cgacggacta ggtgaaatcg
     9421 ttactgacaa agctcgtcca gtagaagcga tggcaccagg tgttatgcaa cgtaaatctg
     9481 ttaacgaacc aatgcaaaca gggctaaaag cgatcgacgc ccttgtgcca atcggacgcg
     9541 gacaacgtga attagtcatc ggtgaccgta aaacagggaa aacttctatt gcgatcgata
     9601 cgatcatcaa ccaaaaaggt caagatatga tctgtatcta tgtagcaatc ggacaaaaag
     9661 attctacagt tcgtacacaa gttgaaacat tgaaaaaata tggcgcaatg gattacacaa
     9721 tcgttgtgaa tgccggtgcg tctcaaccag caccattgct ttatatcgca ccatatgctg
     9781 gtactgcaat gggtgaagaa ttcatgtaca acggtaaaca tgtattgatc atttttgatg
     9841 atctttcaaa acaagctgtt gcttatcgtg aactttcctt gctgcttcgc cgtccaccag
     9901 gtcgtgaagc ttatccaggg gatgttttct acttacactc acgcttattg gaacgtgcag
     9961 caaaattaag tgatgattta ggtggcggtt caatgactgc cttaccattt gttgaaacac
    10021 aagctggaga tatctctgct tatatcccaa caaacgttat ttcgatcact gatggacaaa
    10081 tcttcttaga aagcgacttg ttctacgcag gtactcgtcc agcggttgat gccggactat
    10141 ctgtatctcg tgtagggggt tctgcgcaaa tcaaagcaat gaaaaaagtt gctgggactt
    10201 tacgtctaga cttagcaagt taccgtgaat tagaagcatt tacacaattc ggttctgact
    10261 tggatgcagc aacacaagca aaactaaatc gtggacgccg tacagtagaa atcttgaaac
    10321 aaaaattgca tgcaccgcta gctgttgaaa aacaagtagt gattttgtat gctttgacac
    10381 atggattctt agatagtatc cctgtagaca gtattttaga cttcgaacat gaattattcg
    10441 aatatctgga taccaatcat tcagatatct ttgaaacaat ccgtacaaca aaagatcttc
    10501 cagaagaaga agcgttgaac agtgcgattc aggaatataa agacatgttc ttagctacaa
    10561 aaggaaataa ttcctctaca gaagataaat tgaaatccat tcaaaatgca tagtgaggtg
    10621 agatgaatgg gtgcttcatt aaatgaaatc aagacacgta tcgcttcaac gaaaaaaacg
    10681 agccaaatca cgcgggcaat gcagatggtg tctgcttcta agttgacaaa atctgaagct
    10741 tcttcacaga agtttcaggt ttatgcaaca aaagtaagag aaatcgttac gcatttgact
    10801 gctactcagt taaatgatat tgcctcagaa aatcctagag gggatatcaa ttacaacagc
    10861 atgttgctca gccgtcctgt taagaaaacg ggttatatcg tcatcactgc agatggcggt
    10921 ttagtcggtg gatataacag ctcgatattg aaacaaacga tgtctatttt agaagaagac
    10981 caccaatcac ccgatgatta cgtgatgatc gcaattggcg gaactggtgc agacttcttt
    11041 aaagcacgcg gtatcaatct agcatatgag ctgcgcaatc tatctgatca gccaagcttt
    11101 gatgaagtcc gcaaaatcgt aaatatggca acaactatgt accaaaacga agtattcgat
    11161 gaattatacg tatgttataa ccatcatatc aattcgctaa ctagtcagtt tcgtgtagaa
    11221 aaaatgctgc caatctcaga cttagatcca gaagaagcaa ctactttcga acaagaatat
    11281 atttttgaac cttcaaaaga agaaatcttg tctcagttat tgcctcagta tgctgaaagt
    11341 ttgatctacg gtgcgatcgt tgacgccaaa acagcagaac atgctgctgg tatgacagca
    11401 atgaaaacag caactgataa tgctgctaat atcattgatg agctgacggt ttcttacaac
    11461 cgtgcaagac aaggggctat tacccaagaa attaccgaaa ttgttgccgg agcttcggca
    11521 ctagaatagt attgagattg gaggaaaaaa gatgagttca ggcaagattg ttcaagtaat
    11581 cggtcccgtt gtcgacgtgg aattttcttt agaccaatcc ttaccagata tcaacaacgc
    11641 attagttgtt tataaaaatg acgaaaataa atcaaaagtt gttcttgaag cagcgttaga
    11701 attaggggac ggagtgatcc ggaccattgc gatggaatca acagatggtt tacaaagagg
    11761 aatggaagtc attgatacag gcaaagcaat ctctgttcct gtaggtaaag aaacattagg
    11821 tcgcgtattc aacgtactag gagatacgat cgatttagaa acacctttcc cagaagatgc
    11881 ggaaagaagc gaaattcata aaaaagcacc agcctttgac gaattaagta caagtacaga
    11941 aattttggaa acagggatca aagttatcga tttgcttgcc ccatatttaa aaggtgggaa
    12001 agtcggacta ttcggtggtg ccggtgttgg taaaaccgta ctgatccaag aactgatcca
    12061 taatatcgcc caagaacatg gtggtatttc tgtatttacc ggtgtaggtg aacgtactcg
    12121 tgaaggtaat gacttgtatt atgaaatgaa agattcagga gttatcgaaa aaacagccat
    12181 ggtgttcgga caaatgaacg aaccaccagg tgcacgtatg cgtgttgctt tgactggttt
    12241 gacgattgcg gaatatttcc gtgatgtaga aggtcaagat gtactgttgt ttatcgacaa
    12301 cattttccgt ttcactcaag ctggatctga agtatcagcc ttgttaggac ggatgccttc
    12361 tgcggttggt tatcaaccaa cattggcaac agaaatgggt caattgcaag aacgtatcac
    12421 atctacgaaa aaaggttcga tcacatcgat ccaagcaatc tatgtgccag ccgatgacta
    12481 cactgacccg gcgccggcaa cagcattcgc ccatttggat gcgacaacca acttggaacg
    12541 taaattaaca gaacaaggga tttatccagc ggtagatcca ttggcttctt cttcaagtgc
    12601 tttagcacct gaaatcgtgg gagaagaaca ttataaagta gcaacagaag tccaacacgt
    12661 attgcaacga tatcgtgaat tacaagatat cattgctatc ttaggtatgg atgaattatc
    12721 cgatcaagaa aaagtgttag tttctcgtgc ccgcagagtc caattcttct tgtcacaaaa
    12781 ctttaacgtg gctgaacaat ttactggtct tccaggttca tatgtccctg tagaagaaac
    12841 agtgaaagga ttccgcgaaa tcttggaagg caaatacgat gatcttcctg aagaagcttt
    12901 ccgaagtgtt ggccgaatcg aagacgtcgt agaaaaagcg aaaacactag gctactagaa
    12961 aggggtgccc catggatcaa tatttaacag taaatgtggt aactcctgcc ggtttggtct
    13021 atgatcacca tgcagctatc gtcgttgcac gtacgacagc tggtgagctt gggattttgc
    13081 ctaaacatgc accaatcatc gttccattga cgattgatga agttcgtgta aaacgaacag
    13141 attcagatac ccacgtcgat tggatcgctg ttaatggcgg aatcatggaa gttcgtgata
    13201 atgttgtttc aatcgttgct gatagtgcgg aacgtgaacg tgatatcgat gtatctcgtg
    13261 cggaacgtgc aaaacaacgc gcagaacgcc aaatcgaaga agcaaaagca aaacaagata
    13321 cgaacgaatt gaaacgggca actgttgcgt tgcaccgagc aatcaatcgt atcaaagtat
    13381 ctaagcactc ataaaatgag atgcggatgt ttatccgtgt ttaagaacag taaaaagagg
    13441 ttatgacaga agtggtcagc ttcaagacat aagaaggaat ttacgaaaat tgcttctcaa
    13501 atttttgtga aatctcagct tatttttcga agaagctact tctggaacac cgtatatcgg
    13561 aggttgtgaa atcagcgttt tgtcctgagt attagagaaa caaatggaaa atagttcctc
    13621 atattttgcc gttttttgag ctaataccga gggtcagatg attgaacacc gtttatttgg
    13681 aataagggac tgcgacaaga ctttt
//
LOCUS       DAZKLD010000079        13433 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.079, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000079 DAZKLD010000000
VERSION     DAZKLD010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13433
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.079"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(93..635)
                     /locus_tag="ACG8KR_001818"
     CDS             complement(93..635)
                     /locus_tag="ACG8KR_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HFQ0201185.1"
                     /translation="MIDPKMLGMIFLINFAYITLNTLRFMLTMKGYRLIAPLVSMVEI
                     TIYIVGLSLVLDRLDNPLNLFVYALGYAVGISVGILIEDKLALGYIMVTTILPANSAE
                     DKSLPGILREHGYGVTQTSAMGLEGERLVLEILSPRKSERELYRLIKEIEERAFIISY
                     EPKYISGGFWTKKVRKRQGK"
     gene            770..1159
                     /locus_tag="ACG8KR_001819"
     CDS             770..1159
                     /locus_tag="ACG8KR_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0201186.1"
                     /translation="MKTVNRVFCASHVLSLLLSIYLGVYSIQQPIEVENIVFQMSLTD
                     GLILSVFFFLILFIGNIFGSVASFLNFSIYPLLSLALGVVGLCSLCLFPEPFSPAFIL
                     FGTLNLFQTTVGAWLLWRSGNLMKIGE"
     gene            1163..1927
                     /locus_tag="ACG8KR_001820"
     CDS             1163..1927
                     /locus_tag="ACG8KR_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFQ0201187.1"
                     /translation="MNPIILKRSVHHIPVLEVVLKELREERIPVVIYYHGWQTSKELV
                     LTQGRKIAQQGIRVILPDAMNHGERKQPVSSIPSYTFWDSIYGNLFEFDTLIKHLEKR
                     GLLHDQLAVGGVSMGGMTTCALLAKHPHIVAGVCLMGSPAPLEYGKEIVRRASEYNYV
                     VPKDYFDLISWVKSYDLSLQPEKLAGRPLFFWHGQADEKIPYIQTERFVEKNRHKSYG
                     KEIVFRSSKTQRHMVQIPLMEEASTFLAKNLIKKYK"
     gene            2056..3003
                     /locus_tag="ACG8KR_001821"
     CDS             2056..3003
                     /locus_tag="ACG8KR_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0201188.1"
                     /translation="MPTLADVAKRANVSKMTVSRVINHPEQVTDELKELVFSAMAELD
                     YRPNIAAKALVTNRSQIIKLFILEEIDTTEPYYMNLLMGIAKRIGKAHYSLQLVTNDA
                     FDVGSCDGYIITGVRKKDFDWIDRLEKPVVLFGENSEGYDYVDSDNEYGTAKATQYAL
                     ERDYQTIIYIGIEVDEPFEISRETGYLSVMKEVDLPSKILRFPNHSTEVERYMTENWA
                     DFTDKTCFVCSSDRLALGAVRGIINAGGHLPEEFGVIGFDGVFLDQISSPKLTTIKQD
                     IIRMGEVCGEMLLKKIEEKGASQGYRRFFPELVIRETTN"
     gene            3133..3873
                     /locus_tag="ACG8KR_001822"
     CDS             3133..3873
                     /locus_tag="ACG8KR_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0201189.1"
                     /translation="MKEIRRKHFRFPAYDDELGVKLTNDKKRVLFDGQEDLLTEQNQS
                     PFEQMKEFHFTNTNTEKIRRKRRATNGLKSAPRKENLEKHKGKLPDYTGHYQSEITST
                     GRKKLFGDSSRSVRQSETRTESKHTTRNASVDTKVRERSYFVPKYVPASIIPDPRESK
                     ISEDHLIESMKKTDDSYLLFDTEPTPYQEKRNGEPSVRTFNRTKTLEETKQSEEKKSK
                     KSHGILERSLQGLIDDQGNSLGENSYFK"
     gene            complement(4164..4832)
                     /locus_tag="ACG8KR_001823"
     CDS             complement(4164..4832)
                     /locus_tag="ACG8KR_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFQ0201190.1"
                     /translation="MKKLNLFTELCGIILGAGIYGLAVTGINIPSKLADGGVTGIALL
                     LHNLFGFAPSVTSLLFNLPLLLISLFLFGKHAFLRTIVGTFALVFFLHLWESLHIHFA
                     VDSLLINSLMTGILSGIGCGLVFRFGGSTGGTDIIYQAIEKYFHIKIGKSLFLITFGI
                     LVVSLLYLDLTHFLYTLLSCSILSYTLNKVKYLEIGSVLPQLEAKQTTETFEPLDPLE
                     QLEH"
     gene            4966..5253
                     /locus_tag="ACG8KR_001824"
     CDS             4966..5253
                     /locus_tag="ACG8KR_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0201191.1"
                     /translation="MRAADLLTILTKSPKKTEYQGVFLAQEQKLVPVTMVQINKANQF
                     IFLFEHGKAPLAMKDIFLFLMKNREKAVYYQSKEELIPLYGVKEIENKVVI"
     gene            5669..7207
                     /locus_tag="ACG8KR_001825"
     CDS             5669..7207
                     /locus_tag="ACG8KR_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HFQ0201192.1"
                     /translation="MNNFIQTLSERKGELLEAAFQHLSISLISLLIAALIAIPLAIWA
                     ANHKKMAEVLLQITGVLQTIPSLALLGLLIPFVGIGTVPALIALVIYALLPIFQNTYI
                     GLAEIDPSIEEAAVAFGMSRMRRLLKVELPIALPVIISGIRTALVLIIGTATLAALIG
                     AGGLGTFILLGIDRNTPVLTLIGAISSALLAIIFSGLIRWLQHKKPRYSIITLAAVVC
                     LFGGVSLYQSALFKNETITIAGKLGAEPEILIQMYKELIEDNTKTEVELKPNFGKTSF
                     LFNALDQGQIDIYPEFTGTVLESLVKVPEAMKSADLTKEKTYDEAEELLNKQFDMTLL
                     KPMAYQNTYALAVKRSFANEHQLKKISDLAAISDQIKAGFTLEFIDRSDGYKGIQEAY
                     GLNFSSVQSMEPSLRYQAINNGDVNIVDAYSTDSQLKEYDLVTLEDDRGLFPAYQGAP
                     LMKKDFAKEHPEIVKALNKLSGKITEDQMIDMNYQVNVEKKKPTEVARIFLKNEGLVK
                     EEDQ"
     gene            7204..8169
                     /locus_tag="ACG8KR_001826"
     CDS             7204..8169
                     /locus_tag="ACG8KR_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0201193.1"
                     /translation="MTKLIEFKHVQKKYDGKYVIDDLNLAIKKGEIFVLVGPSGSGKT
                     TTLKMINGLSKPSAGDIYFKGKSLNEYNLQKMRWNMGYVLQQIALFPTMTVKQNIEVI
                     PEMLGWEKQKRADRVDELLQKVGLSPDIYRDRMPRELSGGEQQRIGIIRAIAASPDVI
                     LMDEPFSALDPISRNSLQELVLSLHEELGTTIVFVTHNMEEAIKLGDRIAFMKDGEII
                     QCDTPEQLLMNPKNDYVRHFFDEPKQTKEWRVEDLVVNGYFLNEIPENARQQVCFDTP
                     MKEVYSLLSVYPSIVIVEKQRSIGSLTSKEIFAFLSREEEGNENI"
     gene            8156..9478
                     /locus_tag="ACG8KR_001827"
     CDS             8156..9478
                     /locus_tag="ACG8KR_001827"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFQ0201194.1"
                     /translation="MKTYDAIIIGSGVSGLSAAYGLKEAGKTVLVVEEDLWGGTCPNR
                     GCDPKKVLLSAVEARNRVKQLSGKGFNEIPTANWEELQKFKRTFTDPVPESRKKQLAE
                     AEIDHLSGTARFLDDSSIEVNEEVFHADYLVLATGQRPTILPVEGKEYLKTSADFLSL
                     PVLPKEIIFIGGGYIAFELATIANAAGSKVTIVHHNQRPLKEFEASLVEEAVHQMEAS
                     GIQFAFGVETQKIISEGTRYRLVGKETELVADMIFCATGRQPNTESLALEQANIVFDK
                     HGIAVNDYLQTSNPKIFACGDIVSRKTPKLTPVATFEGNYVAKRITDATSEPIKYPII
                     PTIVYASPKLAEVGVTKSHASSSDQVVEMDLTSWFTYHRVNEPVAKAELTFDQQNYLI
                     GAAVISEQADELIDDLTLVINQKLTKKELDSYIMGYPTLASDLSYLLK"
     regulatory      9541..9645
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            9719..10462
                     /locus_tag="ACG8KR_001828"
     CDS             9719..10462
                     /locus_tag="ACG8KR_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0201195.1"
                     /translation="MKKNLYPAVSMAFILIIWQIVVIVWKIPAFILPGPAAVFSALAA
                     DAQVLLSHSVTTLNEAVLGLVIASVLSFITALAMDYWKFLEMSFYPLLVISQMLPIMV
                     LGPLLTLWFGFGMLPKVILVVLISYFPIVVSFSDTLRKVSKEQIIFLKTMGADTGKIY
                     RIYKIPAGLSGFFSGLKVAATYCVSGAVVGEWLSAQAGLGYYMIRVKNSYQIDKVFAA
                     IICVILLSLLLNGACSLLKKGYYRMLYIF"
     gene            10477..11469
                     /locus_tag="ACG8KR_001829"
     CDS             10477..11469
                     /locus_tag="ACG8KR_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFQ0201196.1"
                     /translation="MKKLWLLLPLLLLLSACGTAKETSSKQEIKDLKKVTLVLDYVPN
                     TNHTGIYLAKEKGYYKEAGLNVQIIEPGDNSTSIGLVGADKAQFGVSYQEDVTYAHAD
                     GQNIPVKAIATVIKHNTSGFATLSDSNIHSPKDFEGKTYAGWQSPSEEAVLKAVMEKD
                     GGDFSKLTMVGSNGEGPESLGKSSDIQWYFEGWDMIKAKEAGIELNYIPLKELDERLD
                     YYTPVIITNDQLIKSDPELVQSFMDATKKGYQEAIKDPNDSAKLLQKYAKENDRTFLE
                     ESQAFLSKNYTDDPENWGLMEEKVWRNYTAFMQENGLIKQDVPSQELFTNQFIK"
     gene            11481..12218
                     /locus_tag="ACG8KR_001830"
     CDS             11481..12218
                     /locus_tag="ACG8KR_001830"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0201197.1"
                     /translation="MSIQISHLNVVIENLKILEDIHFTIPKDTFTSIVAPSGAGKSTL
                     LKTLTGVQPMTSGTIKVDDQKVTGLHTAFSYMPQEDMLLPWLSVYQNVTLYQKINHLT
                     INEEQVRDYLDIFGLNGYEHFLPEQLSGGMKQRTALLRTIMNPSSYLLLDEPFGALDA
                     MTRGQMQDWMLKLPKKAKRTTLLVTHDIEEAIYLSDRILVLSARPAHVIAEIQVPEKR
                     RSREWLLQQSELKQTIYQLLAGELDVK"
     gene            12208..12930
                     /locus_tag="ACG8KR_001831"
     CDS             12208..12930
                     /locus_tag="ACG8KR_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HFQ0201198.1"
                     /translation="MLSDAHCHLDGSQSLALLQQEHTILTIINCDSPEEWKENRQLAA
                     SKTQALSYGIHPWKADSYTFEQVEPFLKKARIIGEIGLDNIWTNVPMTTQKKVFERQL
                     AFAAINEKPVVLHTKGCEKEILNYIQAYPNHYLIHWYSSLDYQQDYIDLGCYFSIGLD
                     LKKNPAVWQLAKAVPIDHLLIETDGLHAAEWALGGIFSEKDYVSLLEEQLKTIAEIKE
                     LSVEEVKKNTYQNLERFIRLGK"
     gene            complement(13014..>13433)
                     /locus_tag="ACG8KR_001832"
     CDS             complement(13014..>13433)
                     /locus_tag="ACG8KR_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HFQ0201199.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEQPVVQETVQTEK
                     QAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQLDSSYL
                     NGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
BASE COUNT         4427 a         2296 c         2789 g         3921 t
ORIGIN      
        1 atatgtcgat gttttttaat caaaataata aataaaaact gtctaacatt taaaatttaa
       61 tttagatatt agacagtttt taagtttgtg agttattttc cttgtcgttt tctcactttt
      121 ttagtccaga aaccacctga aatatatttt ggctcataag aaatgataaa agcacgctct
      181 tcgatttctt tgatcaggcg atacagttca cgttcgcttt ttctaggaga aagtatttcg
      241 agaactagcc gctcaccttc cagccccatt gcagaagtct gtgtcacacc ataaccgtgt
      301 tctctcagta tacccggcaa acttttatct tctgcgctat ttgctggcaa aatcgttgtg
      361 accatgatgt atcctaatgc caatttgtct tcaatcaaaa ttccgacact gatacctact
      421 gcgtaaccta acgcatagac aaataaattc agtggattat ctaaacgatc taaaacaagg
      481 gataagccga cgatataaat cgtgatctcg accatactga caagtggagc aatcagtcga
      541 tagcctttca ttgtcaacat gaaacgtaac gtgttcagag tgatataagc aaaattaatg
      601 aggaagatca tccccaacat ttttggatca atcatttgta aaactccttt catttatata
      661 acaacaatat caaaaaaaat cgtaagtgaa gaaaaattga aactttatac atacaaaaaa
      721 atgataaaat gattataata gaattataaa gataggttag gtgattaaaa tgaaaacggt
      781 caatcgtgtg ttttgtgcca gccacgtgct aagtctcctt ctcagtatat acttaggtgt
      841 ctattcgatt caacagccaa ttgaggtaga gaacattgtt tttcagatgt cgctgacaga
      901 tggtttaatt ttaagcgttt ttttcttttt gatactgttt attggtaata ttttcggtag
      961 cgttgccagt tttttgaatt tttctattta tccactcctt tcgctagcat taggagtggt
     1021 gggactttgt tctctgtgct tatttcccga gccattcagt cctgcattta tattatttgg
     1081 tacactgaac ctgttccaga caactgttgg agcatggttg ctatggcgat caggaaattt
     1141 aatgaaaata ggtgagtaac aattgaatcc aattatattg aaacgttctg tccatcatat
     1201 tccagtgtta gaagttgttc tgaaagaatt aagagaggaa cgtattcccg ttgtcatcta
     1261 ttatcacgga tggcagacaa gtaaggagtt agttttgacg caaggaagaa aaattgcgca
     1321 gcagggcatt cgtgtgatcc tgccagatgc gatgaatcat ggggagcgca aacagccggt
     1381 ttcttctatt ccatcctata cattttggga tagtatctac ggaaatctgt ttgaatttga
     1441 cacacttatc aagcatttag aaaaaagagg tcttctacat gatcagcttg cagttggcgg
     1501 ggtttcgatg gggggaatga caacatgcgc cttgttggct aaacatcctc atatagtagc
     1561 gggcgtgtgt ttgatgggtt cccctgcacc tttggaatat ggaaaagaaa tcgtaaggcg
     1621 cgcatcggaa tataattatg tcgtaccaaa agactatttt gatttgattt catgggttaa
     1681 gagctatgat ttgtcattgc agccggaaaa gttagctgga cgtcctctgt tcttttggca
     1741 tggacaagct gacgaaaaaa tcccttatat acaaacggaa cgatttgtag aaaaaaatcg
     1801 gcataagtct tacggaaaag aaattgtttt tcgttcctca aaaacacagc gacatatggt
     1861 acaaatacca ctaatggaag aagcttccac ttttttagca aaaaatttga taaaaaagta
     1921 taaataattg aaacggaaaa tcactcatga cgatagagga ttttccgttt tttattttgg
     1981 gctataataa actctgttag gaaaatttgg tataattgat agtaagaatg acaacatgtt
     2041 agaaaggaag agagcatgcc cactttagca gatgttgcaa aacgtgccaa tgtttcaaaa
     2101 atgacggtct ctcgagtcat caatcatccg gaacaggtga ctgacgaatt gaaagagtta
     2161 gttttctcag caatggctga actggattac cgtccaaata ttgctgcaaa agcacttgtc
     2221 acgaatcggt cgcagatcat caaattattt atactggaag aaatcgacac aactgagccc
     2281 tattacatga atttgctgat gggaatcgct aaacggatcg gtaaagcgca ttattctctc
     2341 caattagtta caaatgatgc cttcgacgtc ggttcttgtg atggatacat catcacaggt
     2401 gtcagaaaaa aagacttcga ttggatcgac cgtttggaaa aaccagttgt cttgtttggg
     2461 gaaaattctg aaggatatga ttatgtagac agcgataatg aatatggtac agcaaaagca
     2521 acgcaatatg ccttagaaag agattatcaa acgattattt atatcggtat cgaagtggac
     2581 gagccttttg aaatttcaag ggaaactggt tatttatctg tgatgaagga agttgatctt
     2641 cctagtaaaa tcttgcggtt tccaaatcat tcgacagaag tagaaagata tatgacagaa
     2701 aactgggcag attttacaga taaaacttgt tttgtatgta gttcagatcg gctagcacta
     2761 ggagcagtaa gagggattat aaatgctgga ggacacttgc ccgaagaatt tggtgtgatt
     2821 ggatttgatg gggtattttt agaccaaatc agttcaccta aattgacaac gatcaagcaa
     2881 gatattatac gtatgggtga agtatgtgga gaaatgttgt taaaaaaaat agaagaaaaa
     2941 ggggcgagtc aaggttatcg tcgttttttt cctgaactag ttatcagaga gacaacgaat
     3001 taagtcttat tactatatat atatttatga aattcatcgc tattttctta aaataatcag
     3061 cgcattgcta ctgaaaacaa tgaatctgtg ctattattga gaaaagttcc atttttgaga
     3121 aggatggttt gaatgaaaga aatcagacga aaacatttcc gttttcctgc gtacgatgat
     3181 gagttaggcg taaaattaac caacgacaag aagcgagtat tatttgacgg acaagaagat
     3241 cttttgactg aacagaatca gtccccgttt gaacagatga aagaatttca tttcacgaac
     3301 acgaacaccg agaaaatccg tcgcaagcgt cgtgcaacaa atggattgaa gtcagctccg
     3361 cgtaaggaaa atttggaaaa gcataaagga aaattaccgg attataccgg ccattaccaa
     3421 tcagaaatca catcgacagg acgaaaaaaa ctttttggtg attcctctcg ttctgtcaga
     3481 caaagtgaaa caaggacaga gtccaagcat actacacgaa acgcatctgt tgacacgaag
     3541 gtgcgggagc gttcttattt tgttcctaaa tatgtacccg catcgattat cccagaccct
     3601 agagaaagca aaatttctga ggatcattta atcgaatcga tgaaaaaaac ggatgactct
     3661 tatctgttgt tcgatacaga accgacgccg tatcaagaaa aaagaaatgg tgagccgtct
     3721 gtacgaacat tcaatcgaac aaaaacgtta gaggaaacaa agcagtcaga agaaaaaaaa
     3781 tcgaaaaaaa gccatggtat cttagaacgc tcattacaag gattgatcga tgatcaagga
     3841 aactctttgg gagaaaatag ctattttaaa taagtgatat acaaatttat attcggctac
     3901 agttggaaag gaaacgctgc ggcgtttcct tattttatac tctatccgaa agtttatatc
     3961 gtatcctcaa atttttttga gtttaagcga attttttctc ccaataagta agaaaataat
     4021 ggcaaaataa aaaaacaaac tattttcgca gatcgtttgc aaaaatagtt tattttttta
     4081 taaagaagat tttactgaat aatatcattt tagtttacat aatgatattt ctgtaaatta
     4141 aataaagtag tgaaaaataa gaatcagtgc tctaactgtt caagaggatc aagtggttca
     4201 aatgtttctg tagtctgttt tgcttccaat tggggcaaaa cagatccaat ttccagatac
     4261 ttcactttat tcaatgtata agatagaata gaacagctaa gtaatgtata cagaaaatgt
     4321 gtgagatcca aataaagcaa ggagacaact aaaataccaa aagtaatcaa gaaaagactt
     4381 ttgccaatct taatatgaaa atatttttca atggcttgat aaatgatatc tgttccgcct
     4441 gtgcttccgc cgaatcgaaa gacgagaccg caaccaatgc cagaaagaat acctgtcatc
     4501 aaactattga tcagtagact atctacagca aaatgtatat gaaggctctc ccataaatgt
     4561 aaaaagaaaa ctagcgcaaa tgttccgacg atcgtccgga gaaaagcgtg ttttccaaaa
     4621 agaaacaatg agataagcaa taaaggtaaa ttaaaaagca gtgaagtgac agaaggagcg
     4681 aatccaaaaa gattatgcaa aagcaaagca ataccagtaa ccccaccatc tgccagctta
     4741 gatggaatat tgattcctgt cacagctaat ccgtagattc ctgcgcctaa aatgattccg
     4801 cacaattctg taaataaatt taattttttc atttctagcc tcttttcttt tgaaaatttt
     4861 cagcaacttc tttattatac tgacttatac tcttacttca agaggaaaat gaaaaaaaat
     4921 tatttttggc tgtttctttt tatttatcaa ataaggagtg gaaaaatgcg agcagcagac
     4981 ttattgacaa ttcttacaaa atcacccaaa aaaacagagt atcaaggagt ttttttggct
     5041 caagaacaaa aactggtgcc tgtgacaatg gtccagataa ataaagcaaa tcaatttatc
     5101 tttttatttg aacacggcaa agcaccactt gctatgaaag acatctttct ttttttgatg
     5161 aagaaccgtg agaaagctgt ttattatcag tcaaaagaag aactcattcc tctttatgga
     5221 gtgaaagaga tagaaaataa agtggtgata taagtaaaaa taagaaaaca agacgtttat
     5281 gatgtcaaaa caaccatttt ttacattttt ggcaaaaaag tgtcatgata cagtataata
     5341 ttcgttgaac aggccaataa aggtgttctc ccccacgaga tttctacttt attggctgtt
     5401 tctttttatt aaaataaaag ttgtgtgtgt aaaaaagata tagctgattc caagtgacaa
     5461 tttcgtatag atttttatgg tattcgtagt gaaaaaacga gaaccaaaaa gttgatccga
     5521 ctttgttgct tggattcttt ttttgtcagc atatttagag gtattttttt cttttttttg
     5581 ttcgtgctgt aaggttaagg caagacaagt tagttgggaa aaaggaaaga aatgtttata
     5641 cttaaagaat aacagaaagg aaggtcgcat gaataatttt atacaaacat taagtgaaag
     5701 aaaaggcgag ttgctggaag ctgcatttca acatctgtcc atttctctca tttctttgct
     5761 gattgcagca ctgattgcta ttcctttagc gatttgggca gccaatcata aaaaaatggc
     5821 agaagtctta ttgcagatca cgggtgtgct ccaaacgatt ccttcacttg ccttattagg
     5881 tttacttatc ccatttgtag ggatcggaac tgttccagca ttgattgcac ttgttattta
     5941 cgctttgctt ccgattttcc aaaacacgta tattggtcta gctgaaattg acccgtctat
     6001 tgaagaagca gcggtagcat ttggtatgtc cagaatgaga cgcttgctga aagtagagct
     6061 tcccatcgca ttacctgtca ttatctcagg tatccgaact gccttagttt taatcatcgg
     6121 tacggctact ttggcagcat tgatcggagc aggtggatta gggacattta ttttgttagg
     6181 tattgaccga aatacacctg ttttgacatt gattggtgcg atcagttctg ccttgcttgc
     6241 aattatattc agcggactga ttcgctggtt gcagcataag aaacctcgat attccatcat
     6301 tactttagca gcagtggtgt gtttatttgg cggcgtttct ttatatcagt ctgccttgtt
     6361 taaaaatgaa accatcacga ttgctggaaa attaggagcg gaacctgaaa tcctgatcca
     6421 aatgtataaa gaattgattg aagacaacac aaaaacagaa gttgaattaa aaccgaattt
     6481 tggcaagacg agttttttgt tcaatgcact ggatcaaggt caaatcgata tctatcctga
     6541 gttcacaggt actgtcctag aaagcttggt gaaggtacct gaagcaatga aaagtgctga
     6601 tttgacaaaa gaaaaaacat atgacgaagc agaagaatta ttgaataagc aattcgatat
     6661 gacattatta aaaccaatgg cttaccaaaa tacgtatgcc ttggcagtca agagatcttt
     6721 tgcgaacgaa catcaactta aaaagatttc cgatttggca gctatctctg atcagatcaa
     6781 agcgggattc acactggaat ttattgatcg ttcagatgga tataaaggaa tccaagaagc
     6841 atatggcttg aatttttcaa gtgtacaaag catggagcca agtttgcgtt accaagcaat
     6901 caataatgga gatgtcaata ttgtggatgc ctattccaca gatagccagc tcaaggagta
     6961 cgatctagtg actctagaag atgatcgagg gttattccca gcttatcaag gcgcaccgct
     7021 gatgaaaaaa gattttgcta aagaacatcc tgaaatcgtt aaagcactga ataaattaag
     7081 cggaaaaata acagaggatc aaatgattga tatgaattac caagtcaatg tcgaaaagaa
     7141 aaagccgaca gaagtagcac gaatcttcct gaaaaatgaa ggattagtaa aggaggaaga
     7201 tcagtgacta aattaatcga gttcaaacat gtgcaaaaaa agtatgatgg aaagtatgtc
     7261 atcgatgacc taaatctggc aatcaaaaaa ggagaaatct ttgttttggt tggtccttcc
     7321 ggtagtggaa aaacaactac tttgaaaatg atcaatggat tatctaaacc atcagcagga
     7381 gacatctact tcaaaggaaa atcgttaaat gaatataatc tgcagaaaat gcggtggaat
     7441 atgggatatg tgctgcagca aatcgctttg ttcccgacca tgacagtcaa gcaaaatatt
     7501 gaagtcatcc ctgaaatgtt aggttgggaa aagcagaaaa gagcagaccg tgtagatgaa
     7561 cttcttcaaa aagttggctt atctcctgat atttatcgag atcgtatgcc tagagagttg
     7621 tctggaggag aacagcaacg gatcgggatc atccgagcta ttgctgcaag cccagatgtg
     7681 attttgatgg acgagccatt tagcgcatta gatccgattt caagaaactc attacaagaa
     7741 ttggttttgt ccttacacga agaattgggc acgaccatcg ttttcgttac gcacaatatg
     7801 gaagaagcaa tcaagctagg tgatcgtatt gcatttatga aggatggaga aattatccaa
     7861 tgtgacacac cagagcagtt attgatgaat cctaaaaacg actacgttcg gcatttcttt
     7921 gatgaaccga aacagacgaa agagtggcga gtagaagatt tagtcgtcaa tggatatttc
     7981 ttaaatgaaa taccagaaaa tgctcgtcaa caagtttgct ttgatacgcc tatgaaagaa
     8041 gtatattcgc ttctttcggt gtatccaagc attgtgatag tagaaaagca aagaagtatt
     8101 gggtcgctga caagtaaaga aatctttgct tttttaagtc gagaggagga aggaaatgaa
     8161 aacatatgat gcaatcatca tcggttcagg tgtgagcggc ttaagtgctg cttatggatt
     8221 gaaagaagca ggtaaaacag tactagtggt cgaagaagat ctatggggcg ggacttgtcc
     8281 taaccgtgga tgtgatccta aaaaagtctt gttaagtgca gtagaggcac gtaatcgggt
     8341 aaaacaattg agtggaaaag gttttaacga gatcccaact gccaactggg aagaacttca
     8401 aaaattcaaa cgaacattta cggatccagt cccagaaagc cgaaaaaaac aattagcaga
     8461 agctgaaatc gatcatcttt caggaaccgc tcgttttttg gatgattcat ctattgaagt
     8521 caacgaagaa gtcttccatg cggattactt agttttggca accgggcaaa gacctacgat
     8581 cttaccagtg gagggaaagg aatacttgaa aactagtgca gactttttat cattgcctgt
     8641 actgcctaaa gaaatcattt ttatcggtgg tggttatatt gcttttgagc tagctacgat
     8701 tgccaatgct gcagggagca aagtaaccat tgtccatcat aatcaacggc cattaaaaga
     8761 atttgaggcg tctttggtag aggaagccgt ccatcaaatg gaagcatctg gcattcagtt
     8821 tgcttttggt gttgaaaccc aaaagatcat ttcagaaggt actcgctatc gtttagtcgg
     8881 aaaagagacg gaattagtag cagatatgat tttttgtgcg actgggagac agccgaatac
     8941 agaatcttta gcacttgagc aagcgaatat cgtctttgat aaacacggaa ttgcagtcaa
     9001 tgattatctg caaacgagca atccaaaaat ttttgcgtgt ggagatattg tttcaaggaa
     9061 aaccccaaaa ttgacaccag tcgccacttt tgaaggaaat tatgtagcta agcggataac
     9121 ggatgcaaca agcgaaccta tcaaatatcc gattatacct acgatcgtct acgccagtcc
     9181 taaacttgct gaagtaggag ttacaaaaag tcacgcctct tcgtcagatc aagtggtaga
     9241 gatggatctt accagttggt tcacctatca tcgtgtcaat gaaccagttg ctaaggctga
     9301 attgaccttt gaccagcaaa attatttgat tggtgctgca gtgattagcg agcaagcaga
     9361 cgaattgatc gatgatttga cactcgtcat caatcaaaaa ttgactaaaa aagagttgga
     9421 tagctatatc atggggtatc cgacattggc aagtgatctc tcctatttat tgaaataatc
     9481 agcatgataa ctaaagcgct tgttgctttt tccttcttgt gctacacttt aaaaaatgaa
     9541 tacctgtttc tggggatgtc gtagacgaca tgagagtaag ttgtgaactt agacccattg
     9601 aacctgtcag ttaatactgg tgaagggaga aacatagcag acgcttgaag cgggttacta
     9661 tgtcgtgccc atttcaagcg ttttttgtta ttcatattag aagaaaggag gtaacaaaat
     9721 gaagaaaaat ctttatccag cagtcagtat ggcattcatt ttaattattt ggcagattgt
     9781 tgtcatagtt tggaaaatac ctgcctttat tttacccgga cctgctgctg tattttctgc
     9841 attagcggca gatgcacaag tattactttc ccattctgtc acgacattaa atgaagcagt
     9901 gcttggtttg gtaatagcta gcgttctttc ttttattact gccttggcaa tggattattg
     9961 gaagttttta gaaatgtctt tttatccgtt actagtgatt tcacaaatgt tgccaatcat
    10021 ggtgttaggg ccattactta ccctatggtt tggtttcggt atgcttccaa aagtgatcct
    10081 tgttgtcttg ataagctatt ttccgatcgt agtttctttt tcggatacat tgaggaaagt
    10141 atcaaaagag caaatcatct ttttgaaaac gatgggggca gatacaggga agatttatcg
    10201 aatatataaa ataccagcgg gtttatccgg cttcttttcc ggtttaaaag tggctgctac
    10261 ttattgtgtc agcggagcag tcgttggaga atggctaagc gctcaagcag gcttagggta
    10321 ttacatgata cgcgtaaaaa acagctacca gatcgataaa gtttttgctg cgatcatttg
    10381 tgtcattcta ttgagtttat tgctgaatgg cgcttgttca ttattgaaaa aagggtatta
    10441 tcgtatgctt tatatttttt aagagaaggg gatcaaatga aaaaattatg gcttttatta
    10501 ccgctactgt tgcttttatc cgcttgtggc actgcaaaag aaacaagtag caaacaagaa
    10561 ataaaggatt tgaaaaaagt gacacttgtt ttggattatg taccgaatac taatcatact
    10621 ggcatctacc tagcaaaaga aaaaggctat tataaagagg ctggtttaaa tgtccaaatc
    10681 atcgaaccag gagataattc gacaagtatt ggtctagtag gtgcggacaa agcacaattt
    10741 ggtgtaagtt atcaagaaga tgtcacttat gctcacgcag atggacagaa tataccagtc
    10801 aaagcaattg ctacagtgat caaacataat acttctggat tcgccaccct atcagacagt
    10861 aacatccatt caccaaaaga ttttgaagga aaaacatatg caggttggca aagtccaagt
    10921 gaagaagctg tcttaaaagc tgtgatggaa aaagacggcg gggacttttc aaaactgaca
    10981 atggtaggaa gcaatggaga agggccagaa agcttaggaa aatcctcaga tatccaatgg
    11041 tactttgaag gttgggatat gatcaaagca aaagaagcgg gtattgaact aaactatatc
    11101 ccattgaaag aattagatga acggttagat tactatactc cggtaattat tacgaatgat
    11161 caactgatca aaagtgatcc agaattggta cagtctttta tggatgccac gaaaaaaggc
    11221 tatcaggaag ctattaagga tccaaatgac agtgctaaac tgttgcaaaa atatgcaaaa
    11281 gaaaatgatc ggacgttttt agaagaatca caagcattct tgtcaaaaaa ttatacggat
    11341 gatcctgaga attggggatt gatggaagaa aaagtttggc gcaattatac agcttttatg
    11401 caggaaaacg gattgatcaa gcaagatgta ccttcgcaag aactattcac taatcagttt
    11461 ataaaatagg agtgatagat atgtcaatcc aaatcagcca cttgaatgta gtcattgaaa
    11521 acctgaaaat attagaagac atccatttca ctatccctaa agataccttt acatcgattg
    11581 tcgcgccaag cggagccggt aagtcaacat tgctgaaaac tttaacagga gtccagccta
    11641 tgacttctgg aacgatcaaa gtagacgatc aaaaagtaac tggtctccat acagcattta
    11701 gttatatgcc gcaagaagat atgttacttc cttggctgag cgtctatcaa aacgttacgt
    11761 tatatcaaaa aatcaatcat ctaacaatca atgaagaaca agtaagggat tatctggata
    11821 tctttgggtt aaacggctat gaacattttt tgccggaaca attatctggc gggatgaagc
    11881 agcgaacggc cttgttacga acaatcatga atccttcttc ttacttgctt cttgatgaac
    11941 catttggagc gttagatgct atgaccagag ggcagatgca agactggatg ttgaagctgc
    12001 ctaaaaaagc aaagcggact actttacttg tgacgcatga tatcgaagaa gcaatttatt
    12061 tatcagacag gattcttgtg ctttccgccc gtccggcaca tgtgattgct gagattcaag
    12121 tgcctgaaaa acgacggtca cgtgaatggc tactgcagca atcagagttg aagcaaacga
    12181 tttatcagtt attggcaggt gaattagatg ttaagtgatg ctcattgtca tttagatggg
    12241 agtcagagtt tagcactcct tcagcaggaa cacacgattt tgacgatcat caattgtgat
    12301 tcgccagaag aatggaagga aaacagacag cttgcagcaa gtaagactca agccttaagt
    12361 tacggtatcc atccttggaa agcagatagc tatacgtttg aacaagttga gccttttttg
    12421 aaaaaagcaa ggatcatcgg tgaaataggg ttagataata tttggacgaa tgtaccgatg
    12481 actacacaaa aaaaagtatt tgagcgacag cttgcttttg cagccataaa tgagaaacct
    12541 gttgtactgc acacaaaagg atgcgaaaaa gagatcttaa attatatcca agcttaccct
    12601 aatcactatt tgatccattg gtattcttct ttggactatc aacaagacta tattgattta
    12661 ggctgttatt tcagcattgg tcttgatttg aaaaagaatc cagcagtctg gcaactagct
    12721 aaagcagtcc caatcgacca cttacttatt gaaacggatg gactacatgc agcagagtgg
    12781 gctttagggg ggattttttc agaaaaagat tatgtctcac tgcttgaaga acagttaaag
    12841 acaatcgctg agatcaaaga gctatcagtt gaagaagtta aaaaaaatac ttatcaaaac
    12901 ttggaacgtt tcattagatt aggtaaataa aaaagagatg tgacacaact agtgtcgcac
    12961 ctctttttgg ctttctttgt ataaaagtaa actgtttgat taataaatga atcttagtac
    13021 caaccgtttg cttcccagaa tgctttcgcc gcttcccaag aaccataacg tgaagtaaca
    13081 tattgttctg ctactctttc ttggtttgca gcagagtagt caccatttaa gtaagaagaa
    13141 tctaattggt aacgtccgat gtaacgtccg ttcgttgctg tgtaagaacc gcttgattct
    13201 ttttgtgcga tccattcttt tgcagagctt gtgcttgctg gagtagcagc agtttctgtt
    13261 acagcaggag caggttgtgt ttcagcaaca ggcgctgctt gtttttccgt ttgaaccgtt
    13321 tcttgtacaa caggttgttc agcttggact ggtgcttctt gaacggctgc ttgaggttca
    13381 gcagctgctt gtgcagagcc ttctgttggg attgttaatt gttgtcctga gta
//
LOCUS       DAZKLD010000080        13314 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.080, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000080 DAZKLD010000000
VERSION     DAZKLD010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13314
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.080"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            24..425
                     /locus_tag="ACG8KR_001833"
     CDS             24..425
                     /locus_tag="ACG8KR_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0201200.1"
                     /translation="MVIDNSKEKERLNKQISAIKTSLEEHFGLKLFQDSVGEDELPDD
                     FNYFILETGEIEMITEPKYSVGQNLYLTFYSENREDLTGDSLDIISLIQNRSIRFQRM
                     DPNHLKLENQDRYIDQLVFTFRRLLKSDCHG"
     gene            418..822
                     /locus_tag="ACG8KR_001834"
     CDS             418..822
                     /locus_tag="ACG8KR_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0201201.1"
                     /translation="MAKNSWELKINGHDELLVRMERYSSESERLINEALKSKGSAIAV
                     DRITEKIPVSEADLRRGHQHAKNSRPLKTQYINLGFIIRPTRKFEYLKYPDLGIGTSK
                     RNQPDEFMRRGLGLALDPITELLIRQFDKLNK"
     gene            834..1442
                     /locus_tag="ACG8KR_001835"
     CDS             834..1442
                     /locus_tag="ACG8KR_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFQ0201202.1"
                     /translation="MAKTKTVVTTFDNVSIKRIAFNFKNAGNAIATNCNGQLDGETEM
                     QTVVKKCGATEVKSKSKPINMTVTITAHVPMEVYRRFNGLKQDGRIKPGIYSYGPDSV
                     GEDFSLAAEIVDDFEENRKLVGMLACTSNTGLTFSIENGADEVAALELETKVMQDEFG
                     KFYHEAIVAELEEDLTDQWMTNLSADVIKKTSVVTTTATQSQ"
     gene            1462..1824
                     /locus_tag="ACG8KR_001836"
     CDS             1462..1824
                     /locus_tag="ACG8KR_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0201203.1"
                     /translation="MNEDYSKIELNDGTILNLEPKLNIKKLLMINRDFNTDEFAKMTV
                     GKGSMDISVIQGAKAVYIAYRQANMTDYISFDEFIDKWDFDMATASYIYQLMMFKQAR
                     DAYQKEFEKANKEKKLQK"
     gene            1827..2009
                     /locus_tag="ACG8KR_001837"
     CDS             1827..2009
                     /locus_tag="ACG8KR_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0201204.1"
                     /translation="MPKLLVETWVDVYSMLTDVFSMPSDLVLSDICLDDILQMAYNKS
                     AYEGWKNYAINQSQKN"
     gene            2026..5457
                     /locus_tag="ACG8KR_001838"
     CDS             2026..5457
                     /locus_tag="ACG8KR_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFQ0201205.1"
                     /translation="MAKKRTEAEVTFIANDDGLKSTLKEISAELTKNRAELKLEQAQL
                     QQTGSESDKLGSKLSSLEKQYELQSQKVEVTSQRLANAKKYYGENSTEVQKLERELIN
                     QQTAQQRLSNEIDKTSNALAQAKGEIQTYESTMQQLDSEQKNVQASASLIESEYKKWQ
                     ATAGQSASEAEKLAKAQEYVSQQSENAEKTIDILRRQLEATQSEFGATSTEAMQMEAK
                     LNDAEREFEELGQAAKNVDTTNLDDIGSKIDMNNLMEASDVLSDIGDKLTELGKQAVD
                     SANSVGSSQSKIQANFGLSKQEAEELTNVARDIYYKGFGESLDQSTDALILVKRNLGD
                     LNNQDLQNITEQAMVLENTMGADMDETLRGVNGLMVNFGLSAQDAMDLMVSGTQNGLD
                     KTHELGDNMAEYSQLWSQMGYSADETFGMLQNGLDAGAYNLDKVNDLVKEMGISLTDG
                     RFEQNMDMFSESTRKAFEEWKNGGGTQKDVINSMIQDFSNMDGQYDQLNKASTIWSAL
                     GEDNAMKVVQSLTDVNHTFDDVSGSAQKMNEDSTTPLQELNGKIAELKDSLAPIGNTI
                     IDALEPVIDFLGKMADAFNNLPQPVQDFIVAIGGLTAAFGILLPAILAVSYLFGPMML
                     IIGGIIAVIAGVIVAIKNWGAITDWFSGLWKKFTDWLGDTWESIKDGASSVWDGVKET
                     WSGFVDWVQDIWQGVSDWFGELWSGLVEGASNIWQGVQETWQTFVDWVSNIWNGVKEV
                     WSIIWADIVGIVQIPWTLITSLIQAGINIIVGIFDVAGQLLGAAWQAVWTPISDFLKN
                     TWDTMTQWISIAWNGIVTTFHTIFDPVVAWWNGIWTAISTTASNIWNSISATASSIWN
                     SIKNTITSLVQAAATVIQNIWSTVSSWLGGIWNSISSTASNIWNSVTSSISNAINAAK
                     SAIQSVWNSISSWISGIWNGIKNTALNLWNGITSTISSKVNDGKNAISSGWSNLTGIV
                     SDIFNNVKSTIANIWEGIKKTVSAPIDWIRDKISGIFDNLNISIPHIPLPEFIMEGSF
                     NPLKGQIPHLRVKWHAKGGIFTKPTLLGGMNGVGEAGPEAVLPLKRSVLQEIGDRILS
                     STSVSSRAQTIQPVNNYEFNFTIDGNADEVTMKQTTQQIIDSITKIQNDNASAWR"
     gene            5508..6245
                     /locus_tag="ACG8KR_001839"
     CDS             5508..6245
                     /locus_tag="ACG8KR_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFQ0201206.1"
                     /translation="MTDCIHSIIDGFPDYLHKLALAERPTIPSPKRQRVETSVLGRLG
                     GLVQDYSFEDMSFTLHYNYLEDVEDHQAFKQSFYIMRHWLNYAKKLEFSDDPNVYYVI
                     QTIDIGDAENDIVEWGEFDVNITAKPFARVQEDVPITVDKPQSFNLLNNSLEESFPKI
                     IITPSATSCQFILNDYVFSFEGLVAGTDVVIDSDLMLCYEEQSDGDILDRSNKMKTMQ
                     YPTLQVDINHFNCTGLSKIQIYRNGLR"
     gene            6255..8546
                     /locus_tag="ACG8KR_001840"
     CDS             6255..8546
                     /locus_tag="ACG8KR_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail spike protein"
                     /protein_id="HFQ0201207.1"
                     /translation="MIDNLITIYDKNDANNLAEHLYDTQGLGALSDWLTATVSNKLNG
                     AEIFQGTYSINGTNADLIVDGRIIQCYVDENRAKQRLRIYYAKTSVIGNTIEVKAEPI
                     FNDIRKSVLNKYDSGTEKITATQAWQNAKVLAKPAIPSQFSFSSLVDTLANVKIEKAN
                     FLEFFGGKEGSILDRFHGEFLKDNNTLRHEKSLGTDHKIKAIYTKNLTGLDLEIDAQS
                     VLVGVYPFISSSSEGEDEITLPEEVIFTDYVDDYPAGYVSFVDFKDKATDVATLREAA
                     KDWLKTNIDKQKPQVSGSIELVPLRHQRGYEKFVDLEKVSMGDGVDVYHPQLKVNMSA
                     RIVEYTFNVLTNSYDKLVVGNVKTNFLENTENNVSNLINDAIDQLKNGGEISDLLNDI
                     VDHQTDMITGQNGGYVLLDPKEAPSRILIMDTPDKNTARNVLQINNAGIGFSKTGING
                     TYETAWTLDGGFNASFITAGEIVGITIRGTTLISDGADYRTSIANGKMTWYSKKVNKD
                     IMELEARDYVSADAGIVSYTMKTGGGFMIRNPQGNLVFSTWDNGNNRPFLSFGAPNFR
                     YSNASYVTSGDGSSLSINGSAGNSWEFKVAGRTMKFTSDGMLTLPGCFFGSWEDGKLA
                     RFEQSTVQVYKDFTVRGTKNSTVPTEHYGQRLLNAYETPEYYFADYGEAVTGDNGKVR
                     VDIDPMFAETVNLSRYMTHVTPTELVLCAVTHEDIDHFIIETSKPNVLVRWNLVAHRL
                     GYEDIRLKEDTAYDSTVLDQKRF"
     gene            8570..10696
                     /locus_tag="ACG8KR_001841"
     CDS             8570..10696
                     /locus_tag="ACG8KR_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0201208.1"
                     /translation="MASSLYNLALDFSKELNYTKAIMARQGDKGITVTVKPFLNGLQM
                     DTSGGTFTLKGTTPSNRYVDNVATSVTSEEVTFSLDGTFMSEAGYYKHCYVEYRKDNQ
                     ILTTQDIIFFSLGVSDISQGQADEYVSQLEELIRKYNETFDAFMAEIKGRVDSLNQQI
                     TDLTGQAKTLQDKLDALKEEISKLGNLQVMYSNSIDFGDYDYSENPNLMPYITEPWVG
                     PLLGNGHTVKDSVKRVITHTKTRTANSGDILSLGLGIPCTAEANNRYLITTLRPSTTY
                     TLSVTMSVGSDWTGETNTIGVRLRYLNEQGGIELPINALIPANVERDKMVTHTFTGIT
                     KDNVTSITNCYVEIFSLNSEYKGTVSVSYDVKLKAHYPNLLDGPYWLGKVPLGENIAD
                     PTVVFPHKTSEYMVYGRRNTENYIADQTYTISMKATKLTVQSFAVYIAAGRVKVGDMK
                     PTEGLANTWELTFTVTKQHIDSGVTNYLEIYQYPSATKGAVQIEWLKLEKGNTRTPNI
                     SEYKYRGTGMRDSNNPKDYVWDLAPEYVEDNLATDIKISEITGKANNYTDGKVSEINS
                     QLTASINEVDTTAKDAQTKANANATAIDELDNKIDERINDTATTTLTVTNGNTGSAKL
                     YREGKTVSIYFVALNGKSSGGNDSTILTIPEGYRPPISFEQLVGSIDRSTLNSAQLSI
                     GADGAIKWRRNSSYGSDYTFAITYTI"
     gene            10713..10862
                     /locus_tag="ACG8KR_001842"
     CDS             10713..10862
                     /locus_tag="ACG8KR_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0201209.1"
                     /translation="MKAAYRPIEPYGFEQIIVNDEEHLPEECTEVEPPIPNWKPKFNY
                     WEGNK"
     gene            10859..11305
                     /locus_tag="ACG8KR_001843"
     CDS             10859..11305
                     /locus_tag="ACG8KR_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0201210.1"
                     /translation="MKNIWKYGRTGGEYAGKVLDDMLVSVPYTDQPPLEGIRADGEPL
                     TIADQMFDPKLNQWIILANALDHNDLNNLKAMYESLENENGDLKQINAKLMLSDVAIK
                     QENTALKEKADSLAQINSKMMLASLQNSKDISEIKEQLNPASKGGE"
     gene            11307..11444
                     /locus_tag="ACG8KR_001844"
     CDS             11307..11444
                     /locus_tag="ACG8KR_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HFQ0201211.1"
                     /translation="MFSFSDVKMMYDWGCFTNEQVMVFVPLCITEEKADKIISKEESA
                     S"
     gene            11482..11775
                     /locus_tag="ACG8KR_001845"
     CDS             11482..11775
                     /locus_tag="ACG8KR_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0201212.1"
                     /translation="MINLGEWGAIAGSITAIVSLILLVIKPITASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHAEKLVEHTQQIKTLFRERRK"
     gene            11772..11996
                     /locus_tag="ACG8KR_001846"
     CDS             11772..11996
                     /locus_tag="ACG8KR_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HFQ0201213.1"
                     /translation="MNNKTFEVLKWFALVIIPALATFVGLVGKALNWQYTDICVVIIT
                     GFGAFLGSVLGVSNRTYKMFSAESEEGGNK"
     gene            11993..13018
                     /locus_tag="ACG8KR_001847"
     CDS             11993..13018
                     /locus_tag="ACG8KR_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition family protein"
                     /protein_id="HFQ0201214.1"
                     /translation="MKKKITITAMSLLTALFLLPINTFAYTINDEYNLAPNQGDSRLA
                     IPNKIILHETGIDAPARNVAANMKNNYNGSNSYTTDVIGDGGIVYRVGEQGYVSWGAG
                     NANPYAPVQIELQRTYDKALFEKNYRAYIEYTRDSAKKYGIPLTLDQGTSLFTKGIIS
                     HLWVTNYVWGNHTDPYGYLSQMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGA
                     ANPTLTDGTNHAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADG
                     IYRPDVNQAYNTSGNVGYHVSFNMRNFPSKKVYVMMRATNDPEGNTKGGAQDFHDKRW
                     YLNIPKR"
     gene            13207..13280
                     /locus_tag="ACG8KR_001848"
     tRNA            13207..13280
                     /locus_tag="ACG8KR_001848"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13241..13243,aa:Ile,seq:cat)
BASE COUNT         4640 a         2085 c         2807 g         3782 t
ORIGIN      
        1 ttatcttgaa agtgtgggtg aatatggtga ttgataattc taaagaaaaa gaacgtttaa
       61 ataagcaaat ttctgctatc aaaacttcct tagaagagca ttttggcctc aaactctttc
      121 aagactccgt aggcgaggat gagctacctg atgattttaa ttacttcatt ctcgaaacag
      181 gagaaataga aatgatcact gagccaaaat atagcgtggg tcaaaatcta tatctaactt
      241 tctattcaga aaatagagaa gatttaacag gagactcact agatattatt tcattgattc
      301 aaaatcgttc gattcgtttt cagagaatgg atcccaatca tttaaaacta gagaaccaag
      361 atcgctatat cgatcaattg gtatttacgt ttagacgatt attgaagagt gattgtcatg
      421 gctaaaaata gttgggagct aaaaataaat ggacatgatg aacttcttgt gcggatggaa
      481 cgctattcaa gcgagagcga acgactgatt aacgaagcat tgaaatcaaa aggttcggct
      541 attgcagtgg ataggattac agaaaaaatt cctgtttctg aagcagattt aagaagaggg
      601 caccaacacg caaaaaatag tcgtccactt aagactcaat acattaattt gggtttcatc
      661 attagaccta caagaaaatt tgagtattta aaatatcctg atttggggat aggtacttct
      721 aaaagaaatc agccggacga atttatgaga agaggattag gtcttgcact tgatccaatt
      781 acagaacttc tgattcgtca attcgataaa ttaaataaat agggggaaca acaatggcta
      841 aaacaaaaac tgtagtaaca acgttcgata acgtaagtat caaacgaatt gcttttaatt
      901 ttaagaacgc aggaaatgca atcgcaacga attgtaatgg acaattagat ggcgaaacag
      961 aaatgcaaac ggtggttaaa aaatgtggag cgacagaagt aaaatcaaaa tctaaaccaa
     1021 tcaatatgac ggtaacaatt actgcacatg taccgatgga agtttatcga cgtttcaatg
     1081 ggttgaaaca agatggacgt attaaaccag gcatttactc ttacggtcct gattccgtag
     1141 gcgaagattt ctcacttgct gcagagatcg tggatgactt cgaagaaaat agaaagttag
     1201 ttggtatgtt agcatgcact tcgaatacag gattaacatt ctctattgaa aatggtgcgg
     1261 atgaagtagc tgcgttagaa ctagaaacaa aagttatgca agatgaattt ggtaaatttt
     1321 atcatgaagc aattgttgca gaacttgaag aagacttaac agatcaatgg atgacgaatc
     1381 tatctgctga tgtgattaaa aaaacttcag ttgtgacaac tacggccact caatcacagt
     1441 aaaaaaaacg gaggtagcga aatgaacgaa gattactcaa aaattgaact aaacgatgga
     1501 acaattttga atttagaacc taaactgaat atcaagaaat tattgatgat caatagagat
     1561 tttaacacag acgagtttgc aaaaatgact gtgggaaaag gatccatgga tatttctgtt
     1621 attcaaggtg caaaggctgt gtatattgct taccgccaag cgaacatgac tgattatatt
     1681 tcattcgatg aatttatcga taaatgggat tttgatatgg ctactgccag ctatatttat
     1741 caattgatga tgttcaaaca agcacgcgat gcttatcaaa aagaatttga aaaagcaaat
     1801 aaggaaaaaa agcttcaaaa gtaaaaattc caaagctctt agttgaaacg tgggtcgatg
     1861 tctattcgat gttgaccgac gttttttcta tgccttcaga tttggtttta agcgatatct
     1921 gtttagatga cattttgcaa atggcttaca acaagagtgc ttatgaagga tggaaaaact
     1981 atgcaataaa ccaatcccag aaaaactaaa gaaaggaggt aaaaaatggc taaaaagaga
     2041 acagaagcag aagtaacttt catagctaac gatgacggat tgaaatctac gttaaaagaa
     2101 atcagtgctg aattaactaa aaatagagca gaattaaaac tagaacaagc tcaattacaa
     2161 cagactggtt ctgaatcaga caagttagga agtaaattat cttctttaga aaagcagtat
     2221 gaattacaaa gtcaaaaagt tgaagtaact agccaacgtt tagccaatgc gaaaaaatat
     2281 tatggagaaa attccaccga agttcagaaa cttgaaagag aactgattaa tcaacaaaca
     2341 gcgcaacaac gtttgtcaaa cgaaattgat aaaacgagta atgcactagc tcaagcaaaa
     2401 ggcgaaatac agacgtacga gtctacaatg caacagttgg atagcgaaca aaaaaatgtt
     2461 caagctagtg cttctctgat tgaatccgaa tacaaaaaat ggcaagcaac tgctggtcaa
     2521 tcagcttctg aagccgagaa attagcgaaa gcccaagaat atgtttctca acaatctgaa
     2581 aatgcggaga aaacgataga tatcctaaga cgacagttag aagctacaca gtctgaattt
     2641 ggcgctacat ccacagaagc aatgcagatg gaagcgaagc ttaatgatgc tgaacgtgaa
     2701 tttgaagagt taggacaagc tgctaaaaat gtagatacaa ctaacttgga cgatatcgga
     2761 agcaaaatag atatgaataa tttaatggaa gcttctgacg ttttaagcga cattggcgat
     2821 aagcttacag aattagggaa acaagcagtg gactctgcta acagtgtagg tagttcccag
     2881 agtaagatac aagctaattt tggtttgtct aaacaagagg ctgaagaatt aacgaatgta
     2941 gccagagaca tttattataa aggttttgga gaatcgttag atcagtccac agatgcattg
     3001 attttggtaa agcgtaattt aggagattta aataatcaag atttacaaaa tatcacggaa
     3061 caagctatgg tcctagaaaa caccatgggc gctgatatgg atgaaacgtt acgtggtgta
     3121 aatggcttaa tggtcaattt cggcttgagt gctcaagatg caatggattt aatggtttcg
     3181 ggtactcaaa acggtttaga taaaacgcac gaattaggcg acaatatggc agaatatagc
     3241 caattatgga gtcaaatggg atattcagct gatgaaacgt tcggaatgct tcaaaatggt
     3301 ttagatgcgg gtgcttataa ccttgataaa gtcaatgact tagttaagga aatgggaata
     3361 tcgttaacag atggtcgatt tgagcaaaac atggatatgt ttagtgaaag tactagaaaa
     3421 gcttttgaag agtggaaaaa tggcggagga acacaaaaag acgttattaa ttccatgatt
     3481 caagatttta gcaatatgga tggtcaatac gaccaattaa ataaagcttc gacaatttgg
     3541 tctgcgcttg gcgaagataa tgcgatgaaa gttgtccaat ctttaactga tgttaaccat
     3601 acatttgatg atgttagtgg atctgcacaa aaaatgaatg aagattctac tactccgttg
     3661 caagagttga acgggaaaat agctgaatta aaggattcat tagctcctat aggcaacaca
     3721 atcatagatg cactcgaacc agtaattgat tttctaggaa agatggctga tgcgtttaat
     3781 aatcttccac aaccagtaca ggatttcatt gttgctatag gtggtcttac agcagcattt
     3841 ggcatactgt taccggcaat actagccgtg tcatatttat ttggtccgat gatgcttata
     3901 attggaggaa ttatagctgt tatagcaggc gttattgtgg caattaagaa ctggggtgca
     3961 attactgact ggtttagtgg tttatggaaa aaatttactg attggttggg tgatacttgg
     4021 gaaagtataa aagacggagc ctcatcagtt tgggatgggg ttaaagaaac ctggtctgga
     4081 tttgtagatt gggttcaaga tatttggcaa ggagtttctg attggtttgg agagctatgg
     4141 agcggattag ttgaaggagc ttccaacatc tggcaaggag tccaagaaac ttggcaaaca
     4201 ttcgttgatt gggtttcaaa tatttggaac ggagtcaaag aagtatggtc gattatttgg
     4261 gcagacattg taggaattgt tcaaatacca tggacattaa taacgtcatt gattcaagct
     4321 ggtattaata ttatcgtggg tatttttgat gtagctggac agttattggg cgcagcttgg
     4381 caagctgttt ggacacctat ttctgatttc cttaaaaata cttgggatac tatgacacaa
     4441 tggataagta tcgcttggaa tgggattgta actacattcc atactatatt tgatccagta
     4501 gtggcatggt ggaatggtat atggacagct attagtacta cggcttcaaa tatttggaat
     4561 tcaattagtg caacagcttc tagtatttgg aacagtatca agaatacaat cactagcttg
     4621 gtacaagcag ctgctacagt aattcaaaat atttggtcaa ctgtatctag ttggttaggt
     4681 ggaatttgga attcaatcag ctctacagca tcaaatatct ggaatagtgt gactagtagt
     4741 ataagcaatg ctataaacgc agctaaaagt gccattcaaa gtgtttggaa tagtatatct
     4801 tcgtggatta gcggaatttg gaacggtatc aaaaatactg ctttgaatct ttggaatgga
     4861 attacaagca ctattagctc taaagtaaac gatggaaaaa atgcaatttc aagcggttgg
     4921 tccaatctaa caggtattgt ttccgacata ttcaataatg ttaaaagtac aattgctaac
     4981 atatgggaag gcatcaaaaa gactgttagc gctccaattg attggattag agacaaaatc
     5041 agtggcattt ttgataattt gaatatttct ataccacata ttccgttacc ggaatttatc
     5101 atggaaggca gctttaaccc gctaaaaggt caaatccccc atttgcgtgt aaaatggcat
     5161 gctaaaggag gtatctttac taaaccaact ttactaggtg gaatgaacgg tgtcggtgaa
     5221 gcaggacctg aagcagtttt acctttgaaa agatctgttt tgcaagaaat tggtgatcgt
     5281 atcttgagta gcacatcagt ttcatctagg gcacaaacga ttcaacctgt gaataactac
     5341 gaattcaatt tcacaattga tggtaacgca gatgaggtta ctatgaagca aacaactcaa
     5401 caaatcattg atagcattac aaaaattcaa aatgataatg cttcggcatg gcgttaaaca
     5461 ggagagtatt tctcctgttt ttttagtatt aaaaaggatg tgaaaaaatg actgattgta
     5521 tacattctat aatcgatgga tttcctgatt atttgcataa attggcttta gctgaaagac
     5581 caaccatacc ttctccaaaa agacagagag ttgaaacttc tgttttaggt aggttaggtg
     5641 gcttagtaca agattactcg tttgaagata tgtcgtttac attgcactat aactatttag
     5701 aggatgtgga agaccatcaa gcgttcaagc aatcgtttta tatcatgcgt cattggttaa
     5761 actatgcaaa gaaattagaa ttctctgacg atcccaacgt ctattacgtt atccagacta
     5821 tcgatattgg ggatgcagaa aacgatattg ttgaatgggg agagttcgat gtaaatatta
     5881 ctgcgaaacc attcgcaaga gttcaagaag atgtacctat aaccgtagat aaaccacagt
     5941 catttaactt gctgaataat agtttagaag aaagttttcc aaagattatc atcactcctt
     6001 cagctacttc atgccagttc atcttaaatg attatgtgtt tagtttcgaa ggcttagtag
     6061 caggaactga tgtagtcatt gatagtgatt tgatgctttg ctacgaagag caatcggacg
     6121 gagatatttt agatcggtcc aacaaaatga agaccatgca atatccgaca ttgcaagtgg
     6181 atatcaatca ttttaattgc actggtttga gcaaaataca aatttatcgt aacgggttaa
     6241 ggtaggtgaa atagatgatc gataatttaa taactattta cgataaaaat gacgcgaata
     6301 atttagctga acatttatat gatacgcaag gtttaggcgc tttgtcagac tggttaacag
     6361 ctactgttag caataaacta aacggagccg agatatttca gggtacttat tcaataaacg
     6421 gaactaatgc agatttgatt gtagacggac gtattattca gtgttatgta gatgaaaatc
     6481 gagcaaaaca gcgtctacgg atttattatg caaaaacttc tgtaataggc aatacgatag
     6541 aagtaaaagc tgaacctatt ttcaatgata taagaaaatc ggtgttgaat aaatatgaca
     6601 gcggaacaga aaagatcact gctactcagg catggcaaaa cgcaaaagtt ttagcgaaac
     6661 cagctatccc ttcgcagttt tctttctcgt cattagtaga tacgcttgct aatgtgaaga
     6721 tagaaaaggc gaatttttta gaattctttg gtggaaaaga gggatctatt ctagatcgat
     6781 ttcatggtga gtttctaaaa gataataaca cattacgtca tgaaaaaagt ctaggtacgg
     6841 atcataaaat caaagcgatt tatactaaaa acttaactgg tcttgactta gagattgatg
     6901 ctcaaagtgt tttagttgga gtttatccat tcattagcag ctcttcagaa ggagaagacg
     6961 agatcactct accagaagaa gttattttca cggattacgt ggatgattat cctgctggat
     7021 atgtttcttt tgttgatttt aaagacaaag cgactgatgt agccacatta agggaagctg
     7081 ctaaagactg gttgaaaaca aacatagata aacaaaaacc acaagtgagt ggttcgattg
     7141 aattagtacc attgaggcat caaagaggct atgaaaaatt tgttgatcta gaaaaagttt
     7201 cgatgggtga cggagtagat gtgtatcatc cacagttaaa agtgaatatg tcagcgagaa
     7261 ttgtggaata tacgtttaat gttctaacca attcatacga taaattagtt gtaggaaacg
     7321 tcaaaacaaa cttcttagaa aacacagaga ataatgtcag caatttgatt aatgatgcca
     7381 ttgatcaatt gaaaaacggt ggcgaaatca gtgatttact caatgatatt gtagatcatc
     7441 aaactgatat gattactggt caaaatggtg gttatgtttt attagatcct aaagaagcgc
     7501 ctagtcgtat tttgattatg gacacaccag ataagaatac cgcaaggaat gttttacaaa
     7561 tcaacaatgc tggtattggt ttttctaaaa ctggcattaa tggaacatat gaaacggcat
     7621 ggacgttaga tggcggattc aatgcctcgt ttattacggc tggtgaaata gtaggaatta
     7681 ctattagagg tactacatta attagtgatg gtgctgatta tagaacaagt attgctaatg
     7741 gcaaaatgac ttggtactca aaaaaagtta acaaagatat tatggagcta gaagcacgtg
     7801 attatgtaag tgctgatgcc ggtattgtat catacaccat gaaaactggt ggtggtttca
     7861 tgattagaaa tccacagggt aacttggttt ttagtacgtg ggataatggt aataacagac
     7921 cgtttctatc ttttggtgcg cccaatttca ggtatagcaa tgctagttat gtaacttctg
     7981 gcgacggtag ttctttaagc attaatggta gtgcgggtaa ctcatgggaa tttaaggtag
     8041 ctggtaggac tatgaaattt actagtgatg gtatgctaac gttaccaggt tgtttttttg
     8101 gttcatggga agatgggaaa cttgctaggt ttgaacaatc aacggtacaa gtatataaag
     8161 attttactgt tagaggtact aaaaactcaa ctgtaccaac agaacattat ggacaacgac
     8221 tattgaacgc ttatgaaact ccagaatatt atttcgctga ttatggggaa gccgttacag
     8281 gtgacaatgg taaagttcgt gttgatattg accccatgtt tgctgaaaca gtaaatctaa
     8341 gtcggtatat gacacatgtg acacctacag aactagtttt gtgtgctgtt actcatgaag
     8401 atattgacca tttcatcatt gaaactagta agccaaacgt attagttaga tggaatttag
     8461 tggcacaccg tctagggtat gaagatatta gattaaaaga ggatacagca tatgatagca
     8521 cagtgcttga ccaaaaacgt ttttaaaacg aagacaagga ggtatataaa tggctagcag
     8581 tttatataat ttggctttag atttcagcaa agaattaaac tacaccaaag ctattatggc
     8641 tcgtcaaggt gataaaggga ttacggtgac ggttaaaccg tttctaaatg gcttgcagat
     8701 ggatacgagt ggcggaacat ttactttaaa aggaacaaca ccatctaacc gttacgtaga
     8761 taatgttgca actagtgtaa ctagtgaaga agtcacgttt tctcttgatg gcacatttat
     8821 gagtgaagca ggatattata aacactgcta cgtagaatat agaaaagaca atcaaatttt
     8881 aacaacgcaa gatatcattt ttttctcact aggagtgtct gacatttcgc aaggccaagc
     8941 cgatgaatat gtttcgcaat tagaagagtt gattcgaaag tacaacgaaa cttttgatgc
     9001 ttttatggct gaaatcaaag gtagagtgga tagcttaaat caacagatta ctgatttaac
     9061 tggtcaagct aaaacgctac aagacaagtt agatgctctg aaagaagaaa tttctaagtt
     9121 aggtaactta caagtgatgt acagtaacag catcgacttc ggggactatg attattcgga
     9181 gaacccgaat ttaatgccat atattacaga accttgggtt ggacctttgt taggtaatgg
     9241 acatacagtt aaagatagtg ttaaaagggt tataactcat actaaaacta gaacagctaa
     9301 ttccggcgac atattatcgc ttggcttagg tattccgtgt acagcggaag ctaataatag
     9361 gtatcttata accactttac gaccatctac gacatacaca ttaagcgtta ctatgagtgt
     9421 cggttcagat tggactggag agaccaatac tataggggtt aggcttagat atttaaatga
     9481 acaaggtggt atagaactgc ccattaatgc attaatacca gcaaatgtag aacgcgataa
     9541 aatggtcact catacattta ctggtataac aaaagataat gtaactagca taacaaactg
     9601 ttatgttgaa atattctcac ttaatagtga atataaagga acggttagtg tcagctatga
     9661 cgttaagtta aaagcacact accctaactt actagatggc ccttactggt taggtaaagt
     9721 tcctttaggc gagaatattg ctgaccctac agttgtcttt ccacacaaaa ctagtgaata
     9781 tatggtttat ggtagacgca atacagaaaa ctatatcgct gaccaaacat ataccatatc
     9841 aatgaaagca actaaactaa cagttcaatc gtttgccgtg tatattgctg ctggccgcgt
     9901 taaagtagga gacatgaagc ctactgaggg gttagctaat acatgggagt taacttttac
     9961 tgtaactaag caacatatag atagtggggt aactaattat ctggaaatat accaataccc
    10021 gtcagctaca aaaggagctg ttcaaattga gtggctcaaa ctagaaaaag gcaatactcg
    10081 aaccccgaat attagtgagt ataaatatcg tggtactggt atgcgtgatt caaacaatcc
    10141 aaaagattat gtttgggatc tagcaccaga atatgtcgaa gataatttgg ccacagatat
    10201 taaaatttct gaaattactg gtaaagcaaa caattatacc gatgggaaag tatcggagat
    10261 taattcgcag ttgactgctt caattaatga agtagacacc acagctaagg atgctcaaac
    10321 aaaagcgaat gctaatgcga ctgctataga tgaattagac aataagatcg atgaacgcat
    10381 taatgataca gctactacca cattaacagt tacaaacggg aataccggat cagcaaagct
    10441 ttatcgtgaa ggaaaaacag tttctatata ttttgtggct ttaaacggaa aaagcagtgg
    10501 tggaaatgat tcaacgatac taacaattcc agaaggctat cggccaccaa ttagttttga
    10561 gcaactggtt ggctcgatag accgttctac tttgaacagt gctcagttat ctattggtgc
    10621 agatggagcc attaaatggc gaagaaactc aagttatgga tcggattata cctttgcaat
    10681 tacttacacg atttagaaaa gcgtgaatcg atatgaaggc agcatataga ccaattgaac
    10741 cttacggatt cgagcaaatc attgtgaatg atgaagaaca tttaccggaa gaatgcacag
    10801 aagtcgaacc accgattcca aattggaaac cgaaattcaa ttactgggag ggaaataaat
    10861 gaaaaacatt tggaaatatg gacgtactgg cggagagtac gcaggaaaag tattggacga
    10921 catgcttgta tccgttcctt acacagatca gccaccgctt gaaggaattc gtgctgatgg
    10981 cgaaccgtta acaatcgctg atcaaatgtt tgatcctaaa ttgaatcaat ggattatttt
    11041 agcgaacgca ctagatcaca acgatttaaa caatctcaaa gcaatgtatg agtcgttaga
    11101 aaatgagaac ggcgatttaa aacagatcaa tgccaaactc atgctaagcg atgtagcaat
    11161 taaacaggaa aatactgcat tgaaagaaaa agcggatagt ttagcacaaa tcaattcaaa
    11221 aatgatgctt gcttcgttac aaaatagcaa agacatttca gaaattaaag agcaactaaa
    11281 tccagcttca aagggaggtg agtagtatgt ttagttttag cgatgtgaaa atgatgtatg
    11341 attggggctg tttcactaac gaacaagtaa tggttttcgt tccgttgtgc attactgaag
    11401 aaaaagcaga taaaatcatt agcaaagaag agagcgcatc ttaattgatg tgctttttat
    11461 tttgattcaa ggagttgtca catgattaat ttaggggaat ggggagcgat agcaggatca
    11521 ataaccgcta tcgtttcttt gattttatta gtaataaaac caattactgc atctttctcg
    11581 aagattactg agactctttc aaaagtaagt cacaatttag atttgctgac taaagattta
    11641 gaatcgagca aatcagatcg attgatgatt catgaagaac taaagaaaca cgatgaaaga
    11701 ttagatacac atgcagaaaa attggtagaa cacacgcaac aaattaaaac tttatttagg
    11761 gagagaagaa aatgaataat aaaacgttcg aagtactaaa atggttcgca ctggtaatta
    11821 ttcccgcact agctactttc gtggggttag ttggtaaagc gctcaattgg cagtacacag
    11881 atatctgtgt tgtcatcatt actggttttg gcgcgttttt agggagtgtg ttgggtgtat
    11941 caaatcgaac ctacaaaatg ttctcggctg aaagcgaaga aggaggaaac aaatgaaaaa
    12001 gaaaattact attactgcga tgagcctgtt aacggctctt tttttattac ccattaatac
    12061 gtttgcttat actattaatg acgagtataa tttagcgccg aatcaaggag actccagatt
    12121 agcaattcct aacaaaatta ttttgcacga aactggaata gatgcaccag caagaaacgt
    12181 agccgccaac atgaaaaata attataacgg aagcaattct tatactacag atgttattgg
    12241 tgacggtggg attgtttacc gtgtgggtga gcaaggatat gtttcgtggg gagctggtaa
    12301 cgctaatcct tatgcgcctg tacagattga attacagcgc acatatgata aagcattgtt
    12361 tgaaaaaaac tatcgagctt atattgaata tacaagagat agtgcaaaaa aatatggaat
    12421 tccattgact cttgatcaag gaacttcttt atttacaaaa ggaatcattt ctcatttgtg
    12481 ggtgacaaat tatgtttggg ggaaccacac agatccatat ggttacttat cgcaaatggg
    12541 agttagcaaa gaaaagcttg cttatgattt agctcatgga tttaccgatg aaaatccaac
    12601 aacttctgaa aacaagcctg tcattgatcc aacacgagct ggtgcagcta atcctacgct
    12661 gacagatgga acaaatcacg cccacattga tcagtttggg gaaatcgaaa acgcaaactt
    12721 gcatgtggct ggatggcaca ttgctaacta taaatacgag tatattttca ttatggacta
    12781 caatactggg aaagaattag ctcgagtaag agctgatgga atttatagac cagatgtaaa
    12841 ccaagcttat aatacttctg gaaatgttgg ttatcatgta tctttcaata tgcgtaattt
    12901 tcctagtaag aaagtctatg taatgatgcg ggcaacgaat gatccagagg gaaacactaa
    12961 aggcggtgcg caagatttcc atgacaaacg ttggtattta aatattccta aacgataaaa
    13021 atagctcctc gttgaggagc attacataac tatattgaca actataaaaa tcattcgata
    13081 aaatagtgat gttatcgcat atcttcacta tcacccataa tagtcacact ccaagctatg
    13141 cgataacagg tttgttgcca cacattctac tggttgattg tttatggctt tatgtggcaa
    13201 caaccagtac ccttagctca gttggtcaga gcagacggct cataaccgtc cggtcgtagg
    13261 ttcgagtcct acagggtaca ttaacgtagc catttgaatc gttctgtgtt agaa
//
LOCUS       DAZKLD010000081        13300 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.081, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000081 DAZKLD010000000
VERSION     DAZKLD010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13300)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13300)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13300
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.081"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            8..2206
                     /locus_tag="ACG8KR_001849"
     CDS             8..2206
                     /locus_tag="ACG8KR_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0201215.1"
                     /translation="MKKSTVFYKIFLPMFLLGAVLVIGFSLFIYQNTYESIESSYLTD
                     KKNLLRQVKTNVEWKIRTIEYSFSTYGSTKNFSDIFKHPLTYTDYSTYSEIRKELNFI
                     ETIVMDDNNYDLVSLAGKWGVINGSLSQLTEEEIEKYQQKYINNKNNLFWQKKNKGIE
                     MVISLPMFEKEKYALGIATIEKHTIEQVVGESGEDILSIENEVGNLFTTQTVKNKEQP
                     TVLTDVDLDYNQLKVIREKDKTYIVLKSDYNNWIYRLEVDSSAIGAMIRNLRIGLVAV
                     SLTLVGLIGLLSYLFSDRFARPISQIQERLNLKSKGFSGKELDLVAQSVSRIIGEKEA
                     LSAHLVTQKPQLETLFVLSLFRNRVERRELDQRLQQFGYSSQNDCYYTALVQIDLLED
                     SHGGERDLLLLAINNMITEIVPKENRMLPIVLNEEMQATIYRMDKDDAEASKKVMEYC
                     RLIQKRVKEYLNLTISIGISNRFETLNESKQSVDRAKEALYYRVNTGPSSIIFYQEIV
                     PAAHEKTLIRYPIEEQNRLFEAIRSGNQEVKNLVHELIDALFAQNKNPLSREVVSVRL
                     INELVQLGQLLGVDSKNFDNMKQIYVKALNNYHPKELEKLVLEQLVLPITECSQAITD
                     HEFKNLSENIMHIVYTEYDRDLSLDVIADRLHYNPNYLSNVFKKETGEKFGDFVQNHR
                     LEVAKKWLKETNLSVKEIAERLQYRNPQNFIRFFKKKESITPGEYRKKYL"
     gene            complement(2664..3536)
                     /locus_tag="ACG8KR_001850"
     CDS             complement(2664..3536)
                     /locus_tag="ACG8KR_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0201216.1"
                     /translation="MKKKVSIGERSFTLFNNTFLILLALICIVPFLNIIATSFASTQE
                     VVAKKFILFPTTFSLDAYRYILSTPTIFRALAVSIGVTGVGTIVSMCATSLMAYGLSR
                     KYLFGRGFVNFLVVFSMLFSGGMIPTFLVVRSLGLVNSYWAMILPVAVNAMNMIIMRN
                     FFQALPDSLEESAKMDGCTDFGVFFKIMLPLALPSIATISLFYAVTYWNTYMTAILYI
                     NDSSKWPIQILLRQIVIVSSGMQAESSAVDVIPPAQTVKMAVIVIATVPMLIVYPFVQ
                     KYFVKGALVGSVKG"
     gene            3836..4810
                     /locus_tag="ACG8KR_001851"
     CDS             3836..4810
                     /locus_tag="ACG8KR_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0201217.1"
                     /translation="MESVKKQEPFQLTLSKKEEKLRRRKKIIAGIKTNKFLYVMILPG
                     VLYFLIFRYLPMGGLVIAFQDYQPFLGIMGSPWVGFKHFIRLFTEPTFFTLLRNTLIL
                     FGLNIFVFFPMPIILSLLLNEVTNKRLKNGIQTIIYIPHFMSWVIIVSITYVFLNVDG
                     GVLNEFLATMGFEKISFLTSPEWTRTVYIGQVIWKELGWSTIIYLSAITAVDTQLYEA
                     AEMDGAGRLRKTWHVTLPAIRPVIITLLILKIGQTLDLGFEHMYLLLNSLNRSVAEIF
                     DTYIYTAGLKNGQLSFSTAMGLFKGVVGLFMVILANKIAKKLGEDGVY"
     gene            4832..5524
                     /locus_tag="ACG8KR_001852"
     CDS             4832..5524
                     /locus_tag="ACG8KR_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HFQ0201218.1"
                     /translation="MLQSLDKINGRLNRILQLVYLNFLWGGLVIAGLGVFTFGPASYA
                     MVAVIRKWLRSKETFPLTKTYFTYFKENYRETLLMSWIYGAIGIVLLVDLSFIHNWYL
                     RIALYIAAFIYVLSALYIFPIMAHYDWKGYFFKMKMAFLFGFSHLHYSLVLILSMVGI
                     YLMIIQTMPGVLTFIGGSLFFSAVTWTANSIFCHLEWKNSYTEKTEENKNIIKTVKEK
                     IYEKSNARKIRS"
     gene            5490..6998
                     /locus_tag="ACG8KR_001853"
     CDS             5490..6998
                     /locus_tag="ACG8KR_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFQ0201219.1"
                     /translation="MKKATLVKLGLSFAGILALAACGSSSSNSSSETEDGKAKITWLN
                     ILHHASPPTDTIVNLIEEKTDTELTFNWIPDASKEERLTTALASDELGDIVTLQIAML
                     KNSSVRNALKSGVFWDVSEYLKDYKNLSQISEDRIEAASIDGALYGVPIQKDYARAGL
                     VIRKDWLDNLGLDVPTTMDELYEVAKQFTENDPDGNGVDDTVGFGDRAPSDIRYTSFK
                     LFTSYFGAPNGWAVDDNGKFTPEFDTEEYMKALNFSRDLFENGYLAQDFAVTQKTDQQ
                     EQFAQGRTGIYTGMVDIKNLKTMGQGIQDDMELVPVNKISDGEGGGYHVWSEGNGIGG
                     LLAFPKSQVKDEAQLKKLLQFVDDLLEEDVYKLMTGGIEGTHYELNDDGSIEFIDMDL
                     WQQEVQPLSSSRPSEVTFSFKDTDPEKEFSNQLIKENEEFAVMDPSVPLDSPTNNEVG
                     SEIEKIVIDATVQYIMGQLDEDGFKKAVEDWKAQGGDRITQEYEEAYKAAAQ"
     gene            7072..8175
                     /locus_tag="ACG8KR_001854"
     CDS             7072..8175
                     /locus_tag="ACG8KR_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFQ0201220.1"
                     /translation="MENWSKKLAKSIMERTPRLYEEKWYKGKWSYDYGVVLKGFQLLW
                     ERTQEKIYFDFIKDNIDYFVQEDGTIRGYSVEEYNIDHVNTGKLFFLLYKETGEEKYK
                     KAAELLSQQLANHPRTSEGAFWHKEIYPYQIWLDGLYMGSPFYAEFIQNFSEEKDYQD
                     VLRQFEISYKHLFDPKTNLLIHAWDEKKVQPWADPKTGLSHHFWSRSIGWYLMAAADT
                     YELLHEETDCSLLKEILEKTLAALLPYQEENSGTWYQVTTETKRKGNYLEASGSSMFV
                     YAMAKGIRLGLLNKEEWLPQVKKSHQGLLDEFVLETKEGWLNLNKNCQVAGLGGADQR
                     DGSYAYYISEPIICNDQKGVGAFLQALIEVEAL"
     gene            8178..9515
                     /locus_tag="ACG8KR_001855"
     CDS             8178..9515
                     /locus_tag="ACG8KR_001855"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HFQ0201221.1"
                     /translation="MYDILTFGASIDELNTEAIQQAIDAAASDGGGTVVVPAGEFLTG
                     ALFLKSNVELHLSAGAVLKFSDDPKDYPVVHSRWEGVHRKVYASCIYAQNVENISVTG
                     FGTLDGNGKKWWHTFRNEPDNLAYPRPKLMSFHNCHRITVKDIKLIQSPSWTINPILC
                     SNATFDNLTILNPADSPNTDGIDPESCKNVRISNCHIDVGDDCIAIKAGTEDTYERIA
                     CENITITNCTMVHGHGGVVLGSEMSGSIRNITISNCIFQETDRGIRLKSRRGRGGIVE
                     DIRVSNIVMDNVMCPFILNLYYFCGPRGKEPYVWEKKAYPIDERTPAFRRIHFSNITA
                     RNVHASAGFIYGLAEQFIQEITFDNIDVSMAQDAVPGVPAMMTGIEEMSRQGFYIGFA
                     GKVSFNQMSIENHEGPAFLLENTKEISIEKFQSNQSENMDELVVRKNFAYAVE"
     gene            9502..10380
                     /locus_tag="ACG8KR_001856"
     CDS             9502..10380
                     /locus_tag="ACG8KR_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HFQ0201222.1"
                     /translation="MQLNDFLGAPSSRHTPSGKRLYREKQNGFMLEYYQLYLNELETV
                     PALYAYPEKEGPFPPVLYLHSHGGDFSVGKSELIHGAPYLASPSFAEVLTGMGYAVWS
                     IDAWGFEERGGISESELFKQFLLTGKTLWGMRIYDVISLIDYLETREDTDTQRLATIG
                     LSMGGLLSWWVTALDSRVKVCIDLAAQVDIEILLLHRRLDHHGFYYYVPNLLTAYTTL
                     AIQEKIAPRPRLSLVGKNDTMCLDEGVLKLRKGLDKKYQSMGSPENFSSFSLTGGHME
                     TQEMRNIWKQFIREHL"
     gene            10377..11300
                     /locus_tag="ACG8KR_001857"
     CDS             10377..11300
                     /locus_tag="ACG8KR_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030599 - pectinesterase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pectinesterase family protein"
                     /protein_id="HFQ0201223.1"
                     /translation="MRMKDKLIIGKNAIDTPTDFETIQEGIDYLAGLPDSHPKVLIVK
                     EGRYDEYIESRLSNFKLVGAGTVVISGNRFAKQEYLDGSQLGTFRSATFFLEGSNVVL
                     ENLHIENTAGPGEKVGQAVALFNSGDRVSLKNCRLSGYQDTLCTGPLPDLQKDGTPFC
                     TPASANPEYCRQTYEYCWIEGTVDFIFGGADAVFHQCHIHSLASKNPGYITAASTPKN
                     QKTGYRFESCTLTAEPDTRNVYLGRPWRPYANVLFEACKIGGHVHPTGWDNWDDKKNE
                     QTVKFSERNNNYETSIKRPEWIHLEKQGVQK"
     gene            11297..11707
                     /locus_tag="ACG8KR_001858"
     CDS             11297..11707
                     /locus_tag="ACG8KR_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HFQ0201224.1"
                     /translation="MKRKWELLLGMVGGSLSLIFFGGLAVTLSNMSASEFKKSYQSLA
                     VDHSTLSLENTFGLLQDMTGLFAVVLFISLAFLAVALFLTAKGKYLTTATGLYFITGF
                     ILLIGTQFIAFPFAFFYFAAGAFSLYRVRMRKGA"
     gene            11711..13279
                     /locus_tag="ACG8KR_001859"
     CDS             11711..13279
                     /locus_tag="ACG8KR_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HFQ0201225.1"
                     /translation="MQTIKVKNPILRGFNPDPNILRVKERYYIIVSSFEWLPGIRVYE
                     SKNLVNWSHTTDILTTQVSLQGNPTNGSIWAPQISYADGLFYLVYTDVKSAGRPFKDV
                     HNYLITASEITGPWSEPIYLNSNGFDPSLFHDLETGKKWLVNELWDYRLDTPNKSSGI
                     VLQEYDAEKKKLDGPIYKIFAGTELAKTEAPHLYFRNGYYYLMTAEGGTGRGHSVTVC
                     RAKNITGPYELDPGFPMLTASDKPASTLQCTGHGSLVQTPSGRWYMTYLCTRPLNGAA
                     ILGRETAIQEVYWTSDDWLRLKDSQTTPMEEIMIETNEEVQQEKDHQFMDTFEGELKK
                     NWNARRILPNADWCDTKCRPGFLRLISGESLQSTFEHHLLAIRQTDFTFDAETAFEYM
                     PNNFNQMAGLVLYLNESNYIYCYVTWNEDKGKCLRMIESENGVAQIEDWTIPLTEKKT
                     FLKVTVKKETAQFHLKDVSTEEWKKVGPQKNMCILSGGFTGNFIGIAVHDLNKKRGSY
                     ADFEFFKYDGKDHL"
BASE COUNT         4444 a         2194 c         2813 g         3849 t
ORIGIN      
        1 gaagaaaatg aagaaaagta cagtatttta taaaatattt ttaccgatgt ttttattagg
       61 agccgtactt gtcataggat ttagtttatt catttaccag aatacctatg aatcgatcga
      121 atcgagttat ttaacagaca aaaagaattt gctcaggcaa gttaaaacta acgttgaatg
      181 gaaaattaga acgattgaat attcgttttc tacgtatggt tcgaccaaaa atttctcgga
      241 catattcaag catccgctga catatacaga ttactccact tattctgaaa tcaggaaaga
      301 actaaatttt atagaaacga ttgttatgga tgataataat tatgatttag tcagtttggc
      361 tggtaaatgg ggagtaatca atggatcgtt gagtcaactg accgaagagg aaattgaaaa
      421 gtatcaacag aagtacataa ataacaaaaa taatttattt tggcaaaaga aaaacaaagg
      481 aatcgaaatg gtgatttctt tgccaatgtt tgaaaaagaa aaatatgcat tgggaattgc
      541 tacgatagag aaacatacaa tagaacaagt tgttggtgaa agtggagaag acatactttc
      601 tattgaaaac gaagttggca acctttttac tactcagaca gtaaaaaata aagagcaacc
      661 aactgtatta acggacgtgg atctagatta caatcagctg aaagtgataa gagaaaaaga
      721 taaaacgtat atagtactga aatcagatta taataattgg atttacagat tagaagttga
      781 ctctagtgca attggcgcaa tgatccgcaa tctgagaatc gggctagttg cagtgtcttt
      841 aacactggta ggactcattg gtcttctttc ctacttattt tcggacagat ttgcccgtcc
      901 gatttctcaa atacaggaac gtctgaatct taaatcaaaa ggattcagtg ggaaagaact
      961 agatttggtc gctcaatcag tcagtcggat cataggagaa aaagaagcgt taagtgcaca
     1021 cttagttacc caaaagccac agctggaaac gctatttgta cttagtttat ttagaaatcg
     1081 agtagaaagg cgagaactag atcagcggtt gcagcaattt ggttatagtt ctcaaaatga
     1141 ctgctattat acagctttgg ttcaaatcga tctgttagaa gatagtcacg gtggggagcg
     1201 tgatttgcta cttttagcga tcaacaacat gatcacggaa attgtgccaa aggaaaatcg
     1261 aatgctgccg atcgttttga atgaagaaat gcaagccact atttatcgaa tggataaaga
     1321 tgatgcagaa gcaagcaaaa aagtcatgga gtattgccga ctcattcaaa aacgtgtaaa
     1381 agaatacttg aatttaacta tcagtatagg catcagtaac cggttcgaaa cgctgaatga
     1441 aagcaagcaa tcggttgatc gggcaaaaga agccctgtat tatcgtgtaa atactggtcc
     1501 aagttcaatt attttttatc aggaaatcgt tcctgctgct catgaaaaaa cacttattag
     1561 atatccgata gaagaacaaa atcgattgtt tgaagccatt cgttctggaa atcaggaagt
     1621 gaagaattta gtacatgaat tgattgatgc attgtttgca caaaataaaa atcctttgag
     1681 tcgggaagta gtttcggtcc gcttgatcaa tgaactcgtt caattaggac agttattggg
     1741 agtagattca aaaaactttg ataacatgaa acaaatttat gtaaaagcac tgaacaatta
     1801 tcatcccaaa gaactagaaa aacttgtttt agaacaatta gttctgccaa tcacagaatg
     1861 ctctcaagca atcactgatc atgaatttaa aaatttgtcg gagaatatta tgcatatcgt
     1921 ctacacagaa tatgatcgtg atctgtcttt agatgtgatc gctgataggt tgcattataa
     1981 ccctaactat ttaagtaatg tatttaaaaa ggaaacagga gaaaaatttg gtgattttgt
     2041 tcaaaatcat cgtttagagg tagcaaaaaa atggttgaaa gagactaacc tctccgtaaa
     2101 agagattgca gaaagattgc aatatcgaaa tccgcaaaat tttattcgtt tctttaaaaa
     2161 gaaggaatcg attacgccgg gagaatatcg aaaaaaatat ttgtgaatca agaggctgtt
     2221 tttcttgtgg actgattcaa gcaataaaag aagttagaat aaccctagta tttgtattta
     2281 tggatagtta aatttcaagg ctaagcacgc aaaaagaact gtgacaaaag tcttgtcaca
     2341 gttctttttt acgaataaat ggcgttcaag agacaggcta tcgacaataa ggcataattt
     2401 tgtgaaatat gggaagctat ttttcaaaat cgcttcttat tgctcctgtg ggttactcgt
     2461 cgcaatgttt gtgagagttt ttatcatcgc tcaaggctga acgtctcttt cacaacctct
     2521 gatacacggt gttccaggag tagattcttc gaaaaataag ccgaaatttc acaaaaattt
     2581 aagaagcaat tttcgtaaat gccttcttat ttcttgaagc tgaccacttc tgacacaact
     2641 accattttgc gttcagtaca taatcaccct ttgacagaac ctacgagtgc tcctttgaca
     2701 aaatatttct gaacaaaagg ataaacaatc agcataggca ctgttgcaat aacgataaca
     2761 gccattttaa ctgtttgtgc tggtggaatc acatcaacgg cactactttc tgcttgcatc
     2821 ccacttgata caatgacgat ttggcgtaaa agaatctgta tcggccattt agatgaatcg
     2881 ttgatataca aaatagcagt catataagtg ttccagtaag taacagcata aaaaagcgag
     2941 atagtagcaa tagacggtaa cgctaatggc agcataattt tgaaaaatac accaaaatct
     3001 gtgcaaccat ccatttttgc tgattcttcc aaactatccg gcaaggcttg gaaaaagttt
     3061 ctcataatga tcatattcat tgcattaaca gccacaggca ggatcatggc ccagtaagaa
     3121 ttgaccagcc ctagagaacg aacaactaag aacgttggaa tcatcccccc gctgaacaac
     3181 atagaaaata caacaagaaa gttgacgaaa cctcgaccaa acaaatactt tctggaaaga
     3241 ccgtaggcca tcagtgaagt tgcacacata ctgacgatcg ttccgacacc tgtaacacca
     3301 atcgaaacag caagtgctct aaaaatggtc ggcgtagaaa gaatgtagcg atacgcgtct
     3361 aaagaaaagg tagtaggaaa aaggataaac tttttcgcga cgacttcttg tgtagaagca
     3421 aaagatgtag cgatgatatt taaaaaagga acgatacaga tcaaagctag caaaattaga
     3481 aacgtattgt tgaaaagtgt aaaacttctt tctcctatag agactttctt tttcatatag
     3541 ataggctcct cctcaaaata aattaaatgt ttctgtttct atatgttcaa tttatcatcg
     3601 gtgaaagatt acgtatataa tcatttccta tgctaagtgt cagatagaaa aaaatagaag
     3661 gtttgctatg cgtagtttat ttgtgaaaaa atacacttta tcgttttgta tagcacaaaa
     3721 atcattgcta tgtaagcgtt tttacttacg gtaaaaatga ttatatacgt aattcagata
     3781 gttatctatc atgaagtcat aagcagcaaa cattcacaaa actaaggagg ggcttatgga
     3841 aagtgtaaag aaacaagaac catttcaact gacgctatcg aaaaaagaag aaaagctcag
     3901 aagacgaaaa aaaataattg caggaatcaa aacgaataaa tttttatatg tgatgattct
     3961 gccgggagtc ctttatttct tgatttttag gtatcttcct atggggggac tcgttattgc
     4021 ttttcaagat tatcaaccat ttttaggaat aatgggaagt ccttgggtcg gtttcaaaca
     4081 tttcattcgt ttgtttacag aaccgacatt ctttacatta ttaagaaata cgttgatctt
     4141 gttcggatta aatatcttcg tgttttttcc gatgccaatc attttgtctt tactattaaa
     4201 tgaagtaacg aacaaacgat tgaaaaacgg gattcaaacg atcatttaca tccctcactt
     4261 catgtcttgg gtaatcattg tttcgattac ttatgtattt ttgaatgttg acggaggggt
     4321 attgaatgaa ttccttgcaa caatgggatt tgaaaaaatc agttttctaa catcccctga
     4381 atggacacgc acagtatata tcggacaagt gatctggaaa gagttgggct ggtctacgat
     4441 catctacctt tcagcgataa ctgcagtcga tacacagttg tatgaagctg cagaaatgga
     4501 tggagcagga cgcttacgca aaacatggca tgttacattg ccggcaattc gtccagtaat
     4561 cattaccttg ctgattctaa aaatcggtca gacattagac ttagggttcg agcatatgta
     4621 cttgttattg aattcattga atcgaagcgt tgcagaaatt ttcgacacct atatttatac
     4681 agctggattg aagaatggac agttaagttt cagtacagca atgggattgt ttaaaggggt
     4741 cgtaggattg tttatggtaa ttttagctaa taagattgcc aaaaagctag gagaagacgg
     4801 cgtttactaa gatagaaaga gagggaaaga tatgctgcag tcattagata aaataaacgg
     4861 aaggctaaat cgtattttac agttggttta tttgaatttt ctgtggggcg gtttagtcat
     4921 cgcaggtttg ggagttttta ctttcggtcc tgcatcgtat gcgatggttg cagtgattag
     4981 aaaatggctg cgctcaaaag aaacgtttcc tttgacgaaa acgtacttca catattttaa
     5041 agaaaactat cgtgaaacct tgctgatgag ctggatatat ggcgccatcg ggattgtttt
     5101 acttgtggat ctttctttca tacacaattg gtatcttagg atcgctttgt atattgctgc
     5161 gttcatatat gtgctctcag cactctatat tttcccaatc atggctcatt atgattggaa
     5221 aggatatttt ttcaaaatga aaatggcatt tttattcgga ttctcacatc ttcattactc
     5281 attagtgttg atattaagta tggtagggat ttatctgatg atcatccaaa caatgccagg
     5341 tgttttaacc tttatcggtg gcagtctctt cttttccgca gtcacgtgga cagcaaattc
     5401 tatattttgc catttggaat ggaagaactc atatacagaa aaaacagaag aaaacaaaaa
     5461 tattattaaa acagtgaagg agaaaattta tgaaaaaagc aacgctcgta aaattaggtc
     5521 ttagttttgc aggaattcta gcacttgccg catgcggcag cagttcaagt aacagttctt
     5581 cagagacaga agatggaaaa gctaaaatca cttggttaaa tattttgcat catgcttcac
     5641 cgcctacaga cacgattgtg aacttgatcg aagaaaaaac cgatacggaa ctgacattca
     5701 actggattcc tgatgcttca aaagaagaaa gattaacaac tgcattggct tctgatgaac
     5761 taggagatat cgttacactt caaattgcga tgttgaaaaa ctcttctgtc cgcaatgcgt
     5821 taaaatcagg cgtattctgg gatgtatcag aatatttaaa agactataaa aatttaagtc
     5881 aaatttctga agatagaatc gaagctgcga gtatcgacgg tgccttgtac ggcgtaccga
     5941 tacaaaaaga ctacgctcgt gctggcttag tcattcgaaa agactggttg gataatctag
     6001 gactagatgt tccaactacc atggatgagc tttatgaagt agctaaacaa tttacagaaa
     6061 acgatcctga tggaaatggt gtggacgaca cagttggttt tggcgatcgt gcgcctagtg
     6121 atattcgtta taccagcttt aaacttttca cttcttattt tggagcacca aatggctggg
     6181 ctgtagacga taatggcaaa ttcactccag aatttgatac agaagagtat atgaaagctt
     6241 tgaatttttc aagagatcta tttgagaatg gctatttggc acaagacttt gcagttaccc
     6301 aaaagactga tcagcaagaa caatttgcac aaggaagaac tggaatctac actggaatgg
     6361 tggatatcaa gaatctgaag acgatgggtc aaggtattca agatgatatg gaattagtgc
     6421 ctgtaaacaa gatctcagat ggtgaaggcg gcggttatca tgtttggtca gaaggaaatg
     6481 gtattggcgg cttgctagca tttcccaaat ctcaagtaaa agacgaagca caattgaaga
     6541 aattacttca atttgttgat gatcttttag aagaagatgt gtataaattg atgactggcg
     6601 gtatcgaagg aacgcattat gaattgaatg atgatggttc tatcgaattt atcgatatgg
     6661 atctttggca acaagaggtg caaccgttat caagctctcg tcctagtgaa gtgacgttct
     6721 ctttcaaaga tacagaccct gaaaaagaat tctctaatca attgatcaaa gaaaatgaag
     6781 aatttgctgt aatggatcct tctgtgccat tggattcacc gacgaacaat gaagtaggat
     6841 cagaaattga aaaaatcgtc attgatgcta cggtgcaata catcatggga cagcttgacg
     6901 aagatggatt caaaaaagct gtagaagact ggaaagcaca aggaggcgat cgaatcacac
     6961 aagagtacga ggaagcatac aaagcagcgg cacagtaata tagacgattg ggacaaatac
     7021 ttcttggtat ttgtcccaaa tcctatttta atttttaaag gagagcaaat aatggaaaac
     7081 tggtcaaaga aattagcaaa aagtatcatg gaaagaacac ctcgcttata tgaagaaaag
     7141 tggtataaag gaaaatggtc ttatgactat ggcgtcgtac ttaaagggtt ccaattactt
     7201 tgggaacgaa cccaagaaaa aatttatttt gatttcataa aagacaatat cgactatttc
     7261 gtgcaagaag atggaacgat ccgtggctac agcgttgaag aatacaatat cgaccatgta
     7321 aatacaggaa agcttttctt tttattgtac aaagaaacag gcgaagaaaa atataaaaaa
     7381 gcagcagaac tcctttccca gcagttggca aaccatccac gcacatccga aggagctttt
     7441 tggcataaag aaatttatcc ttatcaaatt tggttggatg gcttatatat gggttctcct
     7501 ttttatgctg aatttatcca gaattttagt gaagaaaaag attaccaaga tgttttgaga
     7561 cagtttgaaa tcagttataa acatctattc gatccaaaaa caaatttgct gattcatgca
     7621 tgggatgaaa agaaggtcca accatgggct gatccaaaaa caggtctatc ccatcatttt
     7681 tggagccgat cgattggctg gtatttgatg gctgcagcag atacctatga attacttcat
     7741 gaagaaacag attgttcttt gctgaaagaa attctagaaa agacactggc tgcattactt
     7801 ccatatcaag aagaaaacag cgggacatgg tatcaagtaa caacagaaac aaaacggaaa
     7861 ggaaattatc tggaagcttc cggcagtagt atgtttgttt acgcaatggc taaaggaata
     7921 cgtttgggac tgctgaacaa agaagaatgg cttccacaag tgaagaaatc acatcaagga
     7981 ttacttgatg aatttgttct ggaaacaaaa gaaggctggc tgaacttgaa taaaaactgt
     8041 caagtagcag ggttaggcgg agcagatcag cgagatggtt catatgctta ctacatcagt
     8101 gaaccaatta tttgtaatga tcaaaaaggc gttggtgcat tcttgcaagc attgattgaa
     8161 gtggaggcat tataaatatg tatgatattt tgacatttgg cgcatcaatt gatgaattga
     8221 atactgaagc aatacaacaa gccattgatg ctgcagcaag tgacggaggc gggaccgtag
     8281 ttgttccagc aggtgaattt ctaacaggag ctttattttt aaaaagcaat gtcgaacttc
     8341 atttaagcgc aggagcggtt ctgaaattct ctgacgatcc aaaagattat cctgttgttc
     8401 attcacgttg ggaaggagtc catcgaaaag tttatgcttc ctgtatctat gctcaaaatg
     8461 tagagaacat ctctgtcact ggtttcggta cattagatgg aaatggaaaa aaatggtggc
     8521 atacttttag aaatgaacca gataatctcg cttatcctcg tcctaaattg atgagtttcc
     8581 ataactgtca tcgaattact gtgaaagata ttaaattgat ccagtcacca agttggacga
     8641 tcaacccgat tctttgcagt aatgcaacgt ttgataactt gacgatcttg aacccagcgg
     8701 attcaccaaa tacagacgga attgatccag aatcatgcaa aaatgtgcgt attagtaatt
     8761 gccatattga cgtgggagat gactgtattg caatcaaggc agggactgaa gatacgtacg
     8821 aaaggatcgc ttgcgaaaat atcacgatca caaattgcac gatggtccat ggacatggcg
     8881 gtgtcgtatt aggaagtgaa atgagtggta gtattcgtaa tattaccatt tcaaattgta
     8941 tcttccaaga aactgatcga ggaatacgat tgaaatctag acgaggtcga ggcgggatcg
     9001 ttgaagatat tcgagtatcc aatatagtca tggataatgt gatgtgtcca tttatactga
     9061 atctttatta tttctgtggt ccgcgaggga aagaaccgta tgtatgggaa aaaaaggctt
     9121 atccgataga tgaacggaca ccagcattca gaaggatcca tttttcgaat atcacggcaa
     9181 ggaatgtcca tgcttctgca ggttttattt atggattagc ggaacaattc atacaagaaa
     9241 tcacatttga caatatcgat gtctcgatgg cccaagatgc agttcccggt gttcctgcta
     9301 tgatgacagg aatagaggaa atgagcagac aaggatttta tattggcttt gcgggtaagg
     9361 tttcctttaa ccagatgtcg attgaaaatc acgagggacc agcatttcta ttagaaaata
     9421 ctaaagaaat cagtattgag aaattccaat caaatcaatc agaaaatatg gatgaactgg
     9481 ttgttcgtaa aaactttgct tatgcagttg aatgattttt taggtgctcc atctagcaga
     9541 cataccccta gtggaaaacg attataccga gaaaaacaaa acggttttat gcttgaatac
     9601 tatcaattgt acttaaatga attggaaaca gtaccagccc tctatgctta tccagaaaaa
     9661 gaaggaccgt ttccgccggt cctttacctg cattctcacg gcggagattt ttcagtcggg
     9721 aaaagtgaat tgatccatgg tgctccttac ttagcttctc catcttttgc agaagtactg
     9781 acaggaatgg gatatgctgt ttggtcgatt gatgcttggg gatttgaaga acgcggaggt
     9841 atttctgaaa gcgagttgtt caaacaattt ttattaacag gaaaaacgtt atggggaatg
     9901 cgtatttatg atgtgatcag cttgatcgat tatttggaga caagggaaga cacagatact
     9961 caacggctcg caacgattgg tctttctatg ggaggcttat taagctggtg ggtgacagca
    10021 ttggattctc gtgtcaaagt ttgtattgat ctagcggcac aggtggacat cgaaatactt
    10081 ttgctacatc gccgcctaga tcatcatggg ttttattact atgtgccaaa tctattgaca
    10141 gcttatacaa cgttagcaat ccaagaaaaa atcgctccgc gccctcgtct tagcttagtt
    10201 ggaaaaaacg atacgatgtg cttggacgaa ggcgtactga agctgagaaa agggctggac
    10261 aaaaagtatc aatcaatggg tagtccagaa aacttttctt ctttttcttt aacaggcggt
    10321 catatggaaa cacaagaaat gagaaatatt tggaaacaat tcataaggga gcatttatga
    10381 ggatgaaaga caaactgata attggaaaga atgctataga taccccgacg gattttgaaa
    10441 ctatccaaga aggaatcgat tatttggcag gcctgccgga tagccatcca aaggtcttga
    10501 tagtaaaaga aggaagatat gatgaatata tagaaagcag attatccaat tttaaacttg
    10561 tgggagcagg aacagtagtt atctccggta atcgttttgc aaaacaggag tatcttgacg
    10621 gtagccagtt agggactttc cgaagtgcta cttttttctt agaaggatcg aatgtcgtat
    10681 tagaaaatct tcacatcgaa aatacagcag gtccaggaga aaaggtagga caggctgttg
    10741 ctttattcaa ttcaggagat cgcgtatctt taaaaaactg tcgactatct ggttatcagg
    10801 ataccttatg cacgggtccg ttgcctgatt tgcaaaaaga tggtactcct ttttgtacac
    10861 cggcttctgc caatcctgag tattgtcgtc agacctatga atattgctgg attgaaggaa
    10921 cggtggattt catatttggt ggggcagatg ccgtttttca tcagtgtcat atccatagct
    10981 tagccagtaa aaatcctgga tacatcacag cagcatctac gccgaaaaat caaaaaacag
    11041 ggtatcgttt tgaaagctgt accttgactg cagaacctga tactagaaac gtctatttag
    11101 gaagaccgtg gcgtccgtat gcaaatgtct tatttgaagc gtgtaagata ggtgggcatg
    11161 ttcacccaac tggttgggat aattgggatg acaagaaaaa tgaacagact gtcaaattct
    11221 ctgaacgtaa taacaattat gaaacatcga tcaaaagacc tgaatggata catttagaaa
    11281 agcagggggt tcagaaatga aaagaaaatg ggaactattg ctaggaatgg tcggtggcag
    11341 tctatctttg attttctttg gcgggttagc agttacttta agtaatatgt ctgcgtcaga
    11401 gtttaaaaaa agttatcagt cgcttgctgt agatcattca actttgtctt tagaaaatac
    11461 atttggatta ttacaggaca tgactggtct gtttgccgtt gtgttgttta tttcacttgc
    11521 ttttttagca gtagcattgt ttttgactgc taaaggaaaa tacttaacaa ctgccactgg
    11581 tttgtatttt ataacaggat tcatattgtt gattggcact cagtttattg cgtttccttt
    11641 tgcatttttt tatttcgcag ctggggcatt cagtttatat cgagtaagaa tgagaaaagg
    11701 agcctgaatc atgcagacta tcaaagtgaa aaatcctatt ttaagaggat tcaatccaga
    11761 tcctaatata ttaagagtga aagagcggta ttacattatt gtctcctctt ttgaatggct
    11821 tccaggaatc cgagtatatg agtcgaaaaa tttagtcaac tggtcacata caacggatat
    11881 tttgaccact caagtatctt tgcaaggaaa tcctacaaat ggaagtatat gggcgccaca
    11941 gatcagttat gctgatggat tgttttatct tgtttacaca gatgtcaaaa gtgcaggccg
    12001 tccgtttaaa gatgtccaca attatttgat cactgcatct gagatcacag gtccttggtc
    12061 cgaaccaatt tatttaaata gtaacggctt tgatccttct ttgttccatg atcttgaaac
    12121 gggaaaaaaa tggcttgtca atgaactatg ggattatcgt ctggacacac cgaataaatc
    12181 cagcggtatc gtattacaag aatatgatgc agagaagaag aagcttgatg gtcccatcta
    12241 taaaatattt gcaggtacag aattagcgaa aacggaggca ccacatttgt attttcgtaa
    12301 cgggtattat tatttgatga cggcagaagg cggtacagga agaggacatt cagttacagt
    12361 ttgccgagca aagaatatta ctggtcctta cgagcttgat cctggttttc ctatgctgac
    12421 agctagtgac aaacctgctt ctacattgca atgtacagga catgggagct tagtacaaac
    12481 cccgtcagga agatggtaca tgacatatct ttgtaccaga ccattaaacg gtgcggctat
    12541 tttgggaaga gaaaccgcta ttcaagaagt gtattggacg tccgacgatt ggttaagact
    12601 taaagatagc cagacaactc cgatggaaga aataatgatt gagacaaatg aagaggtaca
    12661 acaagaaaaa gatcatcagt ttatggatac atttgaagga gagctaaaga agaactggaa
    12721 tgccagacgc attttgccaa atgctgattg gtgtgataca aaatgtagac caggtttttt
    12781 acgtctgatt tccggtgaat ctcttcaatc taccttcgaa catcatttgt tagctattcg
    12841 gcaaacggat tttacttttg atgcggagac agcatttgaa tacatgccta ataattttaa
    12901 tcagatggct ggactggtac tgtacttaaa tgaatccaat tatatttatt gttatgttac
    12961 ttggaatgag gacaagggaa aatgcctacg tatgatagaa tcagaaaacg gagtagcaca
    13021 aatagaagat tggacgattc cactcacaga aaagaagact tttttgaaag tcactgtaaa
    13081 gaaagagaca gcacagtttc atttgaaaga cgtatcaact gaagaatgga agaaagtagg
    13141 tccgcaaaaa aacatgtgta tactctcagg agggtttact ggaaatttta tcgggatagc
    13201 agtgcatgat ctaaataaaa aaagaggaag ttatgctgat tttgagtttt tcaagtatga
    13261 tggaaaagat catctgtaat ttaattcttt aataaatgag
//
LOCUS       DAZKLD010000082        13167 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.082, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000082 DAZKLD010000000
VERSION     DAZKLD010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13167)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13167)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13167
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.082"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..608
                     /locus_tag="ACG8KR_001860"
     CDS             <1..608
                     /locus_tag="ACG8KR_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0201226.1"
                     /translation="ENQLFAGDVLEIVLNDQAGEAAAKGVQNKPSTNDEIGNHNPSDT
                     PVVYHDAPPFAPATKIVVEGGLSFLAPSKLDCGQIKATGLTQEAFGQMDIQEKLWIYD
                     QRETKSPWILTIKLSQPFTTDAGFSMQDVLYFKKAEEYQLVNEEELEIVRGQNSTNVQ
                     TDYSEYLNNGRAFKLVLTKENQIAGNYSAEMTWTLADAPTE"
     gene            628..1656
                     /locus_tag="ACG8KR_001861"
     CDS             628..1656
                     /locus_tag="ACG8KR_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HFQ0201227.1"
                     /translation="MNRIWNKSHRNNCSLLLILLLGFILWQTPVHASEFNFAVDFELP
                     ANQIDKNKTYINVRMEPGKTQEIPYTVFNRTDKSVTVETLIHSATTNSNGVIEYGESK
                     SKFDETLSYDMKNLAKTVGEITVPPQSAVTETLSLKAPEKELAGVVAGGITFQEKKEN
                     VSNKSTVGVVNQFSQVKAILIRNSEKESSPNLKLKKTYADQSDGRNVIKANLQNPNAS
                     YMFGLTTSVEIYKKETKEPYLSIKKEKIDVAPNTNFDLLVPLEGKKLAPGHYQAVVKG
                     AWKEKRWNLHTDFEITDDAADQLNEKDIDLKNQPETFDWRWLILVGVILIGLFAGLFI
                     LKERRKKK"
     gene            1653..2411
                     /locus_tag="ACG8KR_001862"
     CDS             1653..2411
                     /locus_tag="ACG8KR_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HFQ0201228.1"
                     /translation="MKKTIALSTVLAGSLLLCTQSVFAENKSSATTEGTISVTKPDAA
                     NPEDVVPVPPVDPNDPDTPFTPNEPNQGTSGLLTIDQAPDLDFGTIKLGTAKTVYAAL
                     QNGTDSTGTATQVQNFVQVTDKSGIYAGWKLSVKRTEFIGTTSDTNKLTGSKVKFKNA
                     VIRNSATNSSGTPSTISGTGAEGIEIPADQAVDLVTAGANEGIGTWIYSLGENVEQGR
                     QSVELFVPVSNYAVDNYVSTFTWSLTDAPTEGKA"
     gene            complement(2497..3237)
                     /locus_tag="ACG8KR_001863"
     CDS             complement(2497..3237)
                     /locus_tag="ACG8KR_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HFQ0201229.1"
                     /translation="MKKYFIIEDNQFHSEWIIKELVNHHIENIVQLTSISQINQLIQL
                     GTIDNHDVFFIDIDLKQSLTGIDVAEQIRKKNAHSNIVFFTAYTNFAVEIINRKILPI
                     GYIRKDRNLSEQIRQVLEQINWKEQQIVNVDKIFIDQKGGSLVLYPREILYIESIKGL
                     KNQVMVKTTTEDKLVNTSLKQLKTELEKYAYFLLLKSYCINLSQLQKIDSAHYVLEFF
                     NGDSLLVGRKIFEKTKERFHDFQKTASS"
     gene            3298..4551
                     /locus_tag="ACG8KR_001864"
     CDS             3298..4551
                     /locus_tag="ACG8KR_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HFQ0201230.1"
                     /translation="MVATVTLYLTFTLILDLGIYLFFSKQLLVKNSFGFIALMVVTLL
                     FPIPKVSFPYLVGLLLMFLFYFTTKNLFISCLISNLFFAITGLSFFLVFDLMDYLGKR
                     HLGTASFFALLIAFIFYLLVKSIDKQTRFLNLLFTYPTEHYFGGICILLSWGVIYLVL
                     GSVHFRSSNYLLLSLLSIVISFFSLFFSIMLISFQVKERQHQESLQLYQINEEYYHHL
                     EEFKHDYKSLLFSLKITLQENKKETLEFLEILEDHSAAIFEKPQIQQLTNIVSPAVRG
                     VFLKFLEKAESEKIPIQVVIPVEVKTIPIDLVVFIRCLSIFISNAFDHRVADQSPITI
                     NLLEEKEGLRFSIANKANPTNLTLSEMVKRGKTSKKNGGLGLYSAKKMLDVYENAELK
                     IEFSKQNHYFFVEMYLASRKSKSAY"
     gene            4846..5100
                     /locus_tag="ACG8KR_001865"
                     /pseudo
     CDS             4846..5100
                     /locus_tag="ACG8KR_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308281.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
     gene            5222..5548
                     /locus_tag="ACG8KR_001866"
     CDS             5222..5548
                     /locus_tag="ACG8KR_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HFQ0201231.1"
                     /translation="MNSMEKVNQFRDERNWRQFHNEKDLAISISLEASELLELFQWKT
                     PEEVKETQLERIKEELADVLIYSYMMADNLQLDLDEIIEEKLIKNNLKYPVEKSAGVR
                     KKYTEF"
     gene            5616..6329
                     /locus_tag="ACG8KR_001867"
     CDS             5616..6329
                     /locus_tag="ACG8KR_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HFQ0201232.1"
                     /translation="MKKKGKAKRWLVNILLFLLLLVGLALIFNEQIKDYLVRETGDKY
                     AIANVTKEDLKKNNDKDVSFDFDAVEPMTTEGVMRSQMRGTDLPVIASIAVPSVSINL
                     PIFKGLDNTSLLYGAGTLSPDQEMGKGNYALASHRATNPELLFTPLENLEMGAKIYLT
                     DLENVYTYKTFFKEKVAPTDTQLLNEVEGKEIVTLITCGDMDAVTRLVVQGELESVTS
                     IKDATDDMRSAFNLETKTF"
     gene            6639..7490
                     /gene="licT"
                     /locus_tag="ACG8KR_001868"
     CDS             6639..7490
                     /gene="licT"
                     /locus_tag="ACG8KR_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator LicT"
                     /protein_id="HFQ0201233.1"
                     /translation="MKIDKILNNNVVISKNGFGEEVVCMGKGLAFQKKIGDEISPEAV
                     QKEFVLRDSFAKNQFQQLMADIPAEEIEWVKQVVELAEDKLKVEFSPNIYLTLTDHLH
                     YAITRAKENIQLPNPLLFETKKFYPKEYQAAKEALLFVKQKTGVELSEDEAGFIAFHF
                     VNSQQGNEDMQVTMTATTMVRDILSIISKFFGIVFDEESLNYQRIITHLQFFAQRYLK
                     GEGSDEQDEFLYALVQGKYPKAFHCVERINEYLRQTQKQEMGIAEQIYLTIHIQRVVS
                     EKKSIQS"
     gene            7611..9476
                     /locus_tag="ACG8KR_001869"
     CDS             7611..9476
                     /locus_tag="ACG8KR_001869"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFQ0201234.1"
                     /translation="MDNQAVGRRVWEAVGGQKNVKSLVHCATRLRFRLKDESLADTQK
                     LKEDPDVIQVVQSGGQYQVVIGSNVADVYQSIVDEEGLAVSEDGKEESKNILNRLIDI
                     ISSIFTPFLGAMAAAGVLKGFLSLATVMGWLTADSGTYQILFAAADGVFTFLPVMLAF
                     TAAKKFKANQFLAVAIAMALVYPAITAVAGAGEAISFFGIPVILSPSGYTSSVIPIIL
                     AVWVQSKLEPFVKKVIPQFLQMILVPLVVLVVMVPLTFLALGPIGTVAGNALGGLFNS
                     IYGFSPIVAGLIMGSLWQVFVMFGMHWGFVPIMFLNIEQYGFDVLMPMLLPAILAQGG
                     AALAVALRTKDTKLRALGISSTVTSLFGITEPTVYGVTLPLKKPFIAACISGGIGGAI
                     IGFSGVKAFSSSLVSLLTIPTFINTVDGVESNVTVAVIATGIAFVLAFVGTLILGFDE
                     QTQDNQLENKHANAGEPITSARHTLKSPLTGKVLPLSEVPDQVFSSGVMGKGIAIDPE
                     VGELVAPADGEITTIFPTGHAVGITTTDGAEILIHIGMDTVELNGNGFEILVKQGDLV
                     KAGDLLIRFDIEAIRAAGYSVITPVVITNTDAFADILELDQKEIIANEDVLAIVK"
     gene            9490..10932
                     /locus_tag="ACG8KR_001870"
     CDS             9490..10932
                     /locus_tag="ACG8KR_001870"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFQ0201235.1"
                     /translation="MQSRFSEKFLWGGATAANQLEGAYDRDGKGLSVADAMPGGKQRF
                     AIIGSEEFDWTIDQEKYIYPNHRGIDHYDRFKEDLALFAEMGFKCYRFSIAWTRIFPN
                     GDEGTPNEAGLEFYDQLIDECLKYDIEPVITISHYEMPLHLAKEYGGWKNRKLIDFYE
                     RFAQTVLERYSSKVKYWMTFNEINSAFHFPALSQGLVKSNGAGEYQNIFQAWHNQFVA
                     SSKAVKIGHELRSDIQIGCMIIYATTYSIDANPVNQAATMIQNQEFIFFCTDVQVRGE
                     YPAYTARTHKKYGVDPEKLEQTEKDFQLLKEYPVDYIGFSYYMSTAINETDPQAATSE
                     GNLLGGVKNPFLEASEWGWQIDPEGLRIALNELYNRYQKPLFIVENGLGAIDHMEEDG
                     TIIDDYRIDYLRRHIEAMANAVADGVDLMGYTPWGCMDLVSASTGEMSKRYGFIYVDL
                     DDEGNGTLNRSKKKSFDWYKEVIASNGVNL"
     gene            11040..11741
                     /locus_tag="ACG8KR_001871"
     CDS             11040..11741
                     /locus_tag="ACG8KR_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFQ0201236.1"
                     /translation="MKRTRVLYLEVADKIKEDIFSGKYPVGSMLPTESELEELFQVSK
                     ITVRKAIELLASDEYVAKKSGRGTTVLSNRPYNRLSKAASFTQILENSRYEVRKETLD
                     LQLVHIKKEDPLFSFFGEQAVCFKRLYYLNNQPYIYFVHYLPQEMMQFSREEFGKESL
                     YRLLTKKGYTIDSFSDDFSAVFLTEEEQEILKTDAQIGIKRIRKSSTQIGEMIEYSEA
                     VYHTELHPYHIDYET"
     gene            11880..12392
                     /locus_tag="ACG8KR_001872"
                     /pseudo
     CDS             11880..12392
                     /locus_tag="ACG8KR_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014566402.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
     gene            12403..12909
                     /locus_tag="ACG8KR_001873"
     CDS             12403..12909
                     /locus_tag="ACG8KR_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lyxose/D-mannose family sugar isomerase"
                     /protein_id="HFQ0201237.1"
                     /translation="MDYKKRVQQMFLDSGVVLKETELERIEYTDFGLGAIETEGLNLF
                     VYENNERYCGKELVLLPYQTCPEHRHPPRDENEGKKETFRCRKGLVYLYVEGKETKKL
                     SCIIPKGQERYYTAKKEIILHAGDQYTIEQNIKHWFQAGATGAVITEFSSSSDDPSDS
                     FTNPAIYR"
BASE COUNT         4413 a         2202 c         2785 g         3767 t
ORIGIN      
        1 cagaaaatca actttttgca ggagatgtat tagaaattgt attaaacgac caagcaggag
       61 aagctgctgc taagggtgtg cagaacaagc caagtaccaa cgatgaaatt gggaatcaca
      121 atccaagtga tacgccagtt gtttatcatg atgccccgcc ttttgcacct gctacgaaga
      181 ttgtagtaga aggtggtctc tcatttcttg ccccttccaa actcgattgt ggtcagatca
      241 aagcaactgg gttgacgcaa gaagcatttg gccaaatgga tatacaggaa aaattatgga
      301 tttatgatca gcgagaaaca aaaagtccat ggatcttaac gataaaatta tctcagcctt
      361 tcacaaccga tgcaggattt tctatgcagg atgtcctata tttcaagaaa gctgaggagt
      421 atcaattagt gaatgaagaa gaactggaaa ttgtcagagg acaaaattca acgaatgtgc
      481 aaacagatta ttcagaatat ttaaataatg gtcgggcgtt taaacttgtc ttaaccaaag
      541 aaaatcagat agctggtaat tactcagcag agatgacatg gacattagca gatgcgccga
      601 cagaataaag atcaaggaga atgattgatg aatcgaatat ggaataaaag tcatcgaaac
      661 aactgctctt tacttctgat ccttctcctt ggattcatcc tttggcagac acctgtccat
      721 gcctccgaat tcaattttgc ggtagatttt gaattaccgg ctaatcagat tgataagaac
      781 aaaacatata taaatgtaag gatggagcct gggaaaacac aggaaatccc ctacacggtc
      841 tttaaccgaa cagataaatc agtaacagtt gagacattga tccactccgc tacgacgaat
      901 tcaaacggtg tgatcgaata tggagaatca aaaagtaaat ttgatgaaac attaagttat
      961 gatatgaaaa acttagcaaa aacagtggga gaaataactg tccctcctca aagtgcggtc
     1021 actgagacac tttcactgaa agctccagaa aaagagctgg ctggtgtggt cgcaggagga
     1081 atcacgtttc aagaaaaaaa agagaacgtt tctaacaaaa gtacagttgg tgtcgtcaat
     1141 cagttttctc aagtgaaagc aatcttgatt cgaaatagcg aaaaagagtc ttccccaaat
     1201 ttgaaattaa aaaaaactta tgcggatcaa agcgatggaa gaaatgtgat taaggcgaat
     1261 cttcaaaatc caaatgcttc ttatatgttc ggcttgacta catcggttga aatctacaaa
     1321 aaagaaacaa aagagcctta tctttcaata aaaaaagaaa aaatagatgt agcacccaat
     1381 acaaattttg atttacttgt cccattagaa ggaaaaaaat tagctccggg acattatcaa
     1441 gcggttgtga agggtgcttg gaaagaaaaa cgctggaatc tccataccga ttttgaaata
     1501 actgatgatg cagcggacca attgaatgaa aaggatattg atttaaaaaa tcagccagaa
     1561 acatttgact ggcgatggct gatacttgtt ggagtcattc ttattggctt atttgctgga
     1621 ttatttattt taaaagaaag aaggaaaaag aaatgaaaaa aacaattgca ttaagtaccg
     1681 ttctagctgg aagtctatta ttatgtacac aaagtgtttt tgctgagaat aagagctcag
     1741 ccactacaga aggaacgatt tctgtaacaa aaccagatgc cgcaaatcct gaagacgttg
     1801 ttccagttcc tcctgttgat ccaaatgatc ccgatacgcc gtttacacct aacgaaccaa
     1861 atcagggaac atccggatta ctgacgattg accaagctcc agatcttgat tttggaacaa
     1921 tcaagttagg tacagcaaaa acagtatatg ctgcattaca aaacggaacg gacagtacag
     1981 ggacagcaac acaagtacag aatttcgtac aagtaacgga taaaagcgga atttatgccg
     2041 gttggaaact atcagtcaaa cgaacagaat ttattggtac aacatctgat acgaataaac
     2101 taactggttc aaaggtgaaa ttcaaaaacg ccgtgattcg aaatagcgca actaatagca
     2161 gtggaacgcc atctactatt tctggtactg gcgctgaagg aattgagatc ccagcagatc
     2221 aggcagtaga tctagtaact gcaggagcaa atgaagggat tggcacatgg atttattcat
     2281 taggagagaa tgtggaacaa gggagacaat cagtggaatt atttgttcct gtatccaatt
     2341 atgccgtaga caactacgtt tcaacattca catggtcgtt aacagatgct cctacagaag
     2401 ggaaagcgta aaatcaaatc aatgacttca gggagtggag ccgattcaag gccaactccc
     2461 tgaaatctgc tatgcttacg tctcagctct tattcatcaa ctcgaagcag ttttttggaa
     2521 gtcatggaaa cgttcctttg tcttttcaaa gatctttcga ccaacgagta aggagtcacc
     2581 attgaaaaat tctaacacat aatgagcaga atcaattttc tgcaattgcg ataagttgat
     2641 acagtaagat tttaatagta aaaagtaagc atacttttcc aactctgtct tcaattgttt
     2701 taaagaagta ttcactaatt tatcttctgt agttgttttg accataactt ggtttttcaa
     2761 acctttgata ctttcaatat ataaaatttc ccgaggatac aggacgagac ttccaccttt
     2821 ttgatcaata aagattttat cgacatttac gatttgctgt tctttccaat tgatctgttc
     2881 tagcacttga cgaatttgtt cagataaatt cctgtctttt ctgatatatc caatcggaag
     2941 gattttccga ttgatgatct ctactgcaaa attcgtataa gcggtgaaaa aaacaatatt
     3001 actatgtgca ttttttttac gtatttgttc ggcgacatca atacctgtga gtgattgctt
     3061 caagtcgata tcgataaaga aaacatcgtg attatcaata gtacccaact ggatcaactg
     3121 attgatttga gagatagaag tgagctgcac gatattctcg atatgatgat tgaccaattc
     3181 tttgatgatc cattcgctgt gaaactggtt atcttctatg ataaaatact ttttcatatg
     3241 caggctccta ttaaaaaaat atgtgtacat tttgaaacaa aacaatggag gaataagatg
     3301 gtcgcaacag tgacacttta tttgaccttt actttgattt tagatttagg tatctattta
     3361 ttcttttcga aacaactgct agtaaaaaac agctttggtt ttatagcttt gatggtggtg
     3421 acacttttat ttcctatccc taaagtgagc tttccttacc tagttggctt attattgatg
     3481 tttctttttt attttactac gaaaaacctg tttatcagct gtttgatcag taatcttttt
     3541 tttgctatca ctggactcag cttttttctg gtttttgatc tgatggatta tttaggaaag
     3601 agacatcttg gaacagcaag tttttttgca ctacttatcg catttatttt ctatctatta
     3661 gtaaaaagta tagacaaaca gacgagattt ctcaacttgc tttttactta tccaactgaa
     3721 cactattttg gaggtatttg tattttatta agctggggcg tgatttactt ggtattggga
     3781 agtgttcact tccgctcatc taattatctg cttctatctc ttttgtcgat agtcatcagt
     3841 tttttttctt tgtttttttc cattatgttg atcagtttcc aagtaaaaga aagacaacat
     3901 caggaatcgc ttcaactgta tcaaataaat gaagaatatt accatcatct agaggaattc
     3961 aaacatgatt ataaaagtct actgttttca ttaaaaatta ctcttcagga aaataaaaaa
     4021 gaaacgttgg aatttttaga aatcttagaa gatcattctg cagctatttt tgaaaaaccg
     4081 caaatacaac agctaacaaa tattgtatcc ccagctgtca ggggtgtctt tttaaaattt
     4141 ctagaaaaag cagaatcgga gaaaatcccg atccaagtag ttattcctgt cgaagtgaaa
     4201 acaatcccga ttgatctggt tgtttttatt cgctgtttat ctatttttat ttctaacgca
     4261 ttcgatcatc gtgtagccga tcagagtccg attacgatca atctgctgga agaaaaagag
     4321 ggattacgtt ttagtatagc gaacaaggca aatccgacga atttgacgct atcggagatg
     4381 gtcaaaagag gaaaaacctc taaaaaaaat ggcgggttag gtctatacag tgcgaaaaaa
     4441 atgttggatg tttacgaaaa tgcagaacta aaaatcgaat tttcaaaaca gaatcactat
     4501 ttttttgtag aaatgtatct ggcatctaga aagagcaaat cagcctactg acttactgtc
     4561 aaaggataag cttgaggaaa gtctacacgg agatagagac aacaacacag catattcaaa
     4621 agaaacagaa gcgtgatgac cgttgtcacc cttctgtttc ttttgctatg tagttaaaca
     4681 aagtagttga cacttccgct acttagtatt agtacgaaag tatacttcat gatactaagt
     4741 agataagaag tgaaaagcgg aacccacgat cgtcaaaatg ctaactgctt tgcttacacc
     4801 agataaaggg aagagcaata tcgcttattt taggcgtgga gaagcaagaa aatccctacg
     4861 atcacacaaa aaatgctgac ccagcagtta agagagttag aacaagatgg cattgtccat
     4921 cgaaaagtct ataaccaagt cccgcctaaa gtagagtact cgctcaccga agaaggaaaa
     4981 tcattaagag agattctagt agcgatgtct gtttggggag aagaacgtat cgaaaaacaa
     5041 aaagcctcag gggaagaagt cacattactg catacacatg gtggctacct aaattactga
     5101 tttctatgac atttatttta aataaaagaa aacaggagtt tttttaaaag catcatctat
     5161 tttttatgta aaaaaatggt atgattaatc aagtaagaaa tgattggaag aaggcaagca
     5221 aatgaatagc atggaaaaag ttaaccagtt tagagatgaa cgtaattggc gacaatttca
     5281 taacgaaaaa gatttggcta tttctatttc tttggaagca tctgaattgt tagaactttt
     5341 tcaatggaaa acgccggaag aagtaaaaga aacacaatta gaacgaatca aagaagaact
     5401 ggcggatgta ttgatctatt catacatgat ggcagacaac cttcagctgg atctagatga
     5461 gatcattgaa gaaaaattga tcaaaaataa tttgaaatat cctgtagaaa aaagcgcagg
     5521 tgtacgaaaa aaatacacag agttttagaa atgtgtcatt cggctgttgg aaagagcaaa
     5581 agaaaaacag taaaatgagt ctggagggag agaaaatgaa aaaaaaagga aaagcaaaaa
     5641 gatggctagt caatatcctc ttgttcctct tgttattagt aggactagct ttgattttca
     5701 atgaacagat caaggattat cttgtcagag agacaggaga taaatatgct atagcgaatg
     5761 taacaaaaga agatctgaaa aagaataacg ataaagatgt cagttttgac tttgatgcgg
     5821 tagaaccaat gacgacagaa ggggtcatga ggtcgcagat gagaggaaca gatctaccag
     5881 tgattgctag tatcgcagtc ccttcagttt caatcaattt accgattttc aaaggcttgg
     5941 ataatacgtc actactttac ggagcaggaa cattatctcc agatcaagaa atgggaaaag
     6001 gaaattatgc cttagcaagc catcgtgcaa cgaaccctga attgctgttt acaccgctag
     6061 aaaatttaga gatgggtgcc aagatctatt tgacagattt agaaaatgtg tatacataca
     6121 aaacattttt taaagaaaaa gttgctccta cagataccca attactgaat gaagtcgagg
     6181 gaaaagaaat tgtcacacta attacttgtg gcgacatgga cgccgtcacg cgactagttg
     6241 tacaaggaga actggaaagt gttacatcga taaaagatgc gacagatgat atgagatctg
     6301 ctttcaatct agagacaaaa acattttagg atactgggac ataagtcgtt acagattaaa
     6361 aacagccaaa ggatatgaat cgataaggaa aaaccaattt gctgaaaaaa agagaaacgc
     6421 aaagaggtgc atctctgatt tggttgtttt ctagatagac ttgtgttcca gtttttttat
     6481 tttttatatg aaaaggcttg cataaataaa aattcgatgg tatgatgatt ttgaataaga
     6541 aatggattgt tactgtacgg caggcaaaac cagaattcag ctaagaaaca gtaggcgaat
     6601 tctaggtttt tttatttttc ttgagagggc gaagcaaaat gaaaattgat aaaattttga
     6661 ataacaatgt cgtcatttcg aaaaatggat tcggggaaga agtcgtttgt atgggaaaag
     6721 gactagcttt tcaaaaaaag attggggacg agatttcgcc agaagcggtc caaaaagaat
     6781 ttgttttaag agattctttt gctaaaaacc aattccagca gctgatggct gatattccgg
     6841 cagaggagat cgaatgggtc aaacaagtag tcgaactggc tgaagataaa ctaaaagttg
     6901 aattttcgcc taatatttat ctgacattga ccgatcatct tcattatgcg atcacacgag
     6961 caaaagaaaa tatccaactg cctaatccat tattatttga aacaaaaaaa ttttatccga
     7021 aagaatatca agcagcgaaa gaggctcttt tgttcgtgaa acaaaagacg ggtgtggaac
     7081 tttctgagga tgaagccggt tttattgcct ttcattttgt gaatagccaa caaggaaacg
     7141 aagacatgca agtgacgatg accgctacca caatggtaag agatatactg agtatcatca
     7201 gtaaattctt cggcatcgtg ttcgatgaag aatctttgaa ttatcagcgt atcattactc
     7261 atctgcagtt ttttgcccag cgttatctca aaggggaagg atctgacgag caagatgaat
     7321 ttctctacgc attagttcaa ggaaagtatc caaaagcgtt tcattgtgtc gaacggatca
     7381 atgagtatct tcgacaaacg caaaaacagg aaatggggat cgcagagcag atttatttaa
     7441 cgattcatat tcaaagagta gtaagtgaga aaaaaagcat ccaatcataa ggattgttac
     7501 tgtatggcag gcaaaacctg gattgaaaaa ataggttcac gaatgtttgt gactgttttt
     7561 ttcaatctgg gtttttattt tttaaataaa aaagaaagaa ggaaaaaaca atggataatc
     7621 aagcagttgg tcgtcgtgta tgggaagcgg taggaggaca aaagaatgtc aaaagtttag
     7681 tacattgtgc tacgcggttg cgattcagat taaaagatga atctttagca gatacgcaaa
     7741 agttaaaaga agacccggac gtgatccaag tcgtacaaag tggcggtcag tatcaagtag
     7801 tgattggcag taatgtagca gatgtttatc aatcgatcgt ggatgaggaa ggtcttgcag
     7861 tttcggagga tggaaaagaa gagtccaaaa atattttgaa tcgtctgatc gatattatct
     7921 ctagtatatt tacacctttt ctaggggcga tggcagccgc cggggtattg aaaggattct
     7981 tatcattggc aacggtgatg ggctggctga cagctgatag cggtacttat caaatattgt
     8041 ttgctgcagc agatggtgtc tttaccttct tacctgtcat gcttgctttt acagcagcta
     8101 aaaagttcaa agccaaccag tttttagcag tagcaattgc tatggcactt gtgtatccag
     8161 ctatcacagc agttgctggt gcgggagaag ctatcagttt ctttgggatc ccagtgattc
     8221 tttcaccatc tggttatact tcttctgtca taccaatcat tctagccgta tgggttcaaa
     8281 gtaaattaga gccattcgta aaaaaagtga ttccgcaatt tctgcaaatg attttagttc
     8341 cgcttgtcgt tttagtagtg atggttccat taacatttct tgctttagga ccaatcggga
     8401 cggttgccgg taatgcttta ggcggtctct tcaactcgat ttatggattt agtccaatcg
     8461 tagcaggtct aatcatggga agtttatggc aagtattcgt gatgttcggc atgcattggg
     8521 gattcgtacc gatcatgttt ttgaatatcg aacaatatgg gtttgatgta ttgatgccaa
     8581 tgcttcttcc agccatcttg gcacaaggtg gggctgcttt agctgtagca ttgcgaacaa
     8641 aagatacgaa acttcgagca ttagggattt cttctacagt gacttcgtta tttggaatca
     8701 ctgaaccaac tgtgtatggt gtgacattac ctttgaagaa accattcatt gccgcttgta
     8761 tttctggagg tattggtgga gctattatcg gattttccgg agtaaaagct ttctccagca
     8821 gtttggtcag cttattgacg attccgactt tcattaatac ggtggatggt gtagagtcaa
     8881 atgtgacagt agcagtaata gctacaggaa ttgcttttgt tcttgcattc gtaggtacat
     8941 tgattttagg atttgacgaa caaacacaag acaatcaatt agaaaacaaa catgcgaatg
     9001 caggagaacc gattacttca gcacggcata ccttaaaaag tcctttaacg ggtaaagtac
     9061 tgccattgag tgaagtacca gaccaagttt tttcttctgg cgtgatggga aaaggtatcg
     9121 ctattgatcc agaagtagga gaactagttg cacctgcaga cggagagatc acgactattt
     9181 ttccaactgg acatgctgta gggatcacaa cgactgacgg agcggagatt ttgattcata
     9241 tcgggatgga tacagtagaa ttaaatggga atggctttga gatcttagtc aaacaagggg
     9301 atctcgtcaa agcaggagat ttattgattc gttttgacat cgaagcaatt agagcagctg
     9361 gctatagcgt aatcactcca gttgtcatta cgaatactga tgcatttgcc gatatacttg
     9421 aattagacca aaaagagatt attgcaaacg aagatgttct ggcaattgta aaataaggag
     9481 gaaatcagga tgcaatcacg tttttcagaa aaatttttat ggggtggagc aactgctgcc
     9541 aatcaattag aaggggctta tgatcgagac ggaaaagggc tatccgttgc agatgctatg
     9601 cctggaggta agcagcgctt tgcgattatc ggcagtgaag aatttgactg gacgattgat
     9661 caagaaaaat atatttaccc gaatcatcga ggaattgatc attacgatcg tttcaaagaa
     9721 gatcttgcct tgtttgcgga aatgggattc aaatgttatc gtttttcgat tgcctggaca
     9781 cgaatttttc caaatggaga cgaaggtact cctaacgaag cgggattaga attttatgat
     9841 cagctgattg atgagtgctt aaaatatgat attgaacctg tgatcacgat ttctcattac
     9901 gaaatgcctc ttcatttggc aaaagaatac ggtggctgga aaaatcgaaa actgatcgac
     9961 ttctatgaac gatttgctca gacagtatta gagagataca gcagtaaagt caaatattgg
    10021 atgactttca atgaaatcaa ttcggccttt cactttccag ctttaagtca aggattagta
    10081 aaaagcaatg gagcaggaga gtaccaaaat attttccaag catggcataa tcaattcgtt
    10141 gcaagtagca aagccgtaaa aatcggccac gaattgcgtt cggatatcca aatcggctgc
    10201 atgattatct atgcaacaac ttacagcatc gatgccaatc cagtcaatca ggcagcaaca
    10261 atgatccaaa accaagaatt catttttttc tgcacggatg tccaagtacg tggagaatac
    10321 cctgcataca cagcgcgtac ccataaaaaa tatggagtcg atccggaaaa attagagcaa
    10381 acggaaaaag acttccagtt gctaaaagaa tatccagtgg actacatcgg atttagttat
    10441 tatatgtcta cggcaatcaa tgagactgat ccacaagcag caacttcaga agggaacttg
    10501 ctgggaggag taaaaaatcc gtttcttgag gcaagtgaat ggggctggca gatcgatcca
    10561 gaagggctga gaatcgcatt gaatgaatta tacaatcgtt accagaaacc attattcatt
    10621 gttgaaaacg gtcttggagc cattgatcat atggaagagg atggcacgat tattgatgat
    10681 tatcggatcg attatttacg tcgtcatatc gaagcgatgg ctaatgcagt agcagacggt
    10741 gtagatttga tgggctatac gccttgggga tgtatggatc ttgtcagtgc atcaacagga
    10801 gaaatgagta aacgttacgg ctttatctat gtggatctag acgatgaagg aaatgggaca
    10861 ctgaatcgat caaagaaaaa atcttttgat tggtataaag aagtaattgc ctcaaatggc
    10921 gtaaacttgt aattaaagca gctgaatgaa aaagaacaca cttctcacat gtgatttgtg
    10981 ttcttttttt attttttatg tgctattctt ttagtaatag taaagcaaag ggtgacgtta
    11041 tgaaaaggac acgtgtacta tacttggaag tcgcagataa aattaaagaa gatattttct
    11101 caggaaaata tccagtcggt tcgatgttac cgacagaaag tgaattagaa gaactgtttc
    11161 aagtcagcaa aatcactgtc cgaaaggcaa tcgaactgct ggcatcagat gaatatgtcg
    11221 caaagaaaag tggtcgtggg acgacggttc taagcaatcg tccttacaat cgattatcca
    11281 aagcggcaag ctttacgcaa atcttggaga actcccgata tgaagtccgg aaagaaacgc
    11341 tggatttgca attggtccat ataaagaagg aagatccttt gttttccttt ttcggtgagc
    11401 aagctgtttg ttttaagcgc ttatattatt tgaacaatca gccttatatt tattttgttc
    11461 attatcttcc acaagaaatg atgcaattca gcagagaaga atttggcaaa gaatctttgt
    11521 atcgtttgct gacgaaaaaa ggatatacta ttgactcatt ttctgatgat ttctcggcag
    11581 tatttttaac agaagaagaa caagaaattc tgaaaacaga cgcacagatc ggcatcaaac
    11641 ggatacggaa atcgtctacg caaattgggg aaatgattga atattcggag gcggtctacc
    11701 atacggagct gcatccttac catattgatt atgaaacata aataaaaaaa gaggttgtga
    11761 aaaagctgcc ctgcatcaag taataagaac agccaaacaa aaatagcttc tcatattagc
    11821 ttctcatatt tttcgtttgg ttaggcttat taccgcagat acccggctgt ttctggatta
    11881 ccatttattc tggataaggg ggtgtggcac gattttcaga aggaatctat gaaccaatca
    11941 ctgtattgat tttagcttcg atggtaaccc cattcggctt gacgatcgct tatttcttag
    12001 ggaaaatcat tcgtaaaaat atattcaatc gtcaagaaat cgatacattg aagaccgctt
    12061 ttcctatggg gatctgtcag atcacagagg gatgtatccc tatcgtattg aacgacttgg
    12121 cacgcaatgt catagcaacg ggagtcggtg gggcagtcgg cggtggattg agcatgtttt
    12181 ggggcgcaga tagccgtatt ccggcatcgg gaatgtttgc agtcgctacg atgaccagac
    12241 catgggcatt cattggtgca ttgttagctg gttcttttgc tacgggaata actattttat
    12301 tattgaaaaa accagttgac ccaaatgcag aagtcatcca agaagaaaaa gaggaagaag
    12361 agatatcttg ggatgacttg acaatcagtt aggaggaaca gaatggatta caagaaaaga
    12421 gtacaacaaa tgttcctaga ttcaggagta gtattgaaag aaacagaatt agaaagaatc
    12481 gaatatacag attttggatt aggagcaatc gagactgaag gattgaattt atttgtttat
    12541 gaaaataatg agcgatactg tgggaaagaa ctggtattat tgccttatca aacttgtcca
    12601 gagcatcggc acccacctcg agatgagaat gaaggaaaaa aggaaacctt tcgttgtcga
    12661 aaaggcttag tatatcttta tgtcgaagga aaagaaacaa aaaaactttc ctgtattatt
    12721 ccaaaggggc aagaacgtta ctatacggca aaaaaagaaa tcatacttca cgctggagat
    12781 cagtacacga tcgaacaaaa tattaaacac tggttccagg caggtgcaac aggcgcggtt
    12841 atcaccgaat tttcttccag cagtgatgat ccaagtgata gttttacgaa tccagctatt
    12901 tatcgttaat acaaaggttt gggaacaagc tgatgaacca aaatgatcaa atttcgatta
    12961 tttggttcgt cggctttttt atttagagaa acatcaggcg ttggtgaaac ataacacgag
    13021 aataaaatgg atttttcatt aataggagaa acaagtgtgt gactatttaa ataatgcgtg
    13081 ttattgacat gcaggacgaa atcccaattt cttctataag taagtcgttt caattttata
    13141 tttactagtt atttattttt ttggtta
//
LOCUS       DAZKLD010000083        13150 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.083, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000083 DAZKLD010000000
VERSION     DAZKLD010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13150)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13150)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13150
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.083"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(80..152)
                     /locus_tag="ACG8KR_001874"
     tRNA            complement(80..152)
                     /locus_tag="ACG8KR_001874"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(117..119),aa:Lys,seq:ctt)
     gene            533..1102
                     /locus_tag="ACG8KR_001875"
     CDS             533..1102
                     /locus_tag="ACG8KR_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HFQ0201238.1"
                     /translation="MEKMRQQLMEWGKELETFRLPHWEELPDLELYMDQVRTLVDRYL
                     SPVIQGEKHPLLTSSMVNNYVKLGLIPAPVKKRYNKEHVAFLLAITTLKQVLTIPEIK
                     EGILFQGKTVGIREAYNLFCDEQEAAVWMVSQLAQGKSHPQKFDQATPVEYIAVRAAT
                     LSFAMKLLAEKTIVLETEYLKEEKTNEKQ"
     gene            1089..1949
                     /locus_tag="ACG8KR_001876"
     CDS             1089..1949
                     /locus_tag="ACG8KR_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFQ0201239.1"
                     /translation="MKNNKIAILVDSGTDVPEELIKKYEMYMIPLKIIYKDQVYTDKI
                     DITSEEIYQRLPEEIPGTSLPDGESITEIFEQIKKDGYEKVLAVTISSGLSGTYNVVR
                     LIAQQQEELEAYVLDTKNIGIGAGFSAIQAAKWLEEGVEWDQLISNLNELVERTKVFF
                     NVATLEYLQKGGRIGLVASIVGTALKLNPIISCNEEGIYYTVGKARGRKKSLDKTVAY
                     VKEKIGNAKVFNLAVAHGDAKEEAIEMEARLKREFPQANQIYFGQISPALVVHTGPGL
                     LGVGVQVLEG"
     gene            2139..2843
                     /gene="yycF"
                     /locus_tag="ACG8KR_001877"
     CDS             2139..2843
                     /gene="yycF"
                     /locus_tag="ACG8KR_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator YycF"
                     /protein_id="HFQ0201240.1"
                     /translation="MKKVLVVDDEKPISDIVKFNLAKEGYDVYTAYDGEEALEKVAEV
                     EPDLILLDLMLPKMDGLEVAREVRKTYDMPIIMVTAKDSEIDKVLGLELGADDYVTKP
                     FSNRELVARVKANLRRGATAAKEPEEAAPAELTIGDLTIHPEAYMVTKRGETIELTHR
                     EFELLFYLAKHLGQVMTREHLLQTVWGYDYFGDVRTVDVTVRRLREKIEDNPSHPNYL
                     VTRRGVGYYLRNPEQE"
     gene            2848..4683
                     /gene="walK"
                     /locus_tag="ACG8KR_001878"
     CDS             2848..4683
                     /gene="walK"
                     /locus_tag="ACG8KR_001878"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HFQ0201241.1"
                     /translation="MKRKVRFFRSVNFKIAITFILILLISIEIIGAYFIRGLERSTIN
                     TFIKDMNQTVESLATTISPELNRKDNADDEEVNANIKRFIENSATSDIIEIRVVDEKG
                     IIRGTTDVNEQSAVGKKNDYVDINDFTTKRYVALDNDDKRVNINVQPILSPTGDAVIG
                     ALYVKSNIEQKYSEINNTAVIFFTASLIAAFISMIVSVLVARSITQPIGEMREQAIRI
                     ARGDYSRKVKVHGQDELGQLADTFNQLAERIEEAQDTMEAERNRLDSVLSHMTDGVIA
                     TDRRGKVITINDMAVSLLDIKNEEAIGQSILTLLDIEEEYTLRKLLEDPNEMVLDRST
                     STLESDQMILRVDFSMIRRESGFISGLVAVLHDVTEQEKTERERREFVSNVSHELRTP
                     LTSMRSYIEALSEGAWKDEEVAPNFLKVTLDETDRMIRMINDLLNLSRMDTGNTQLQL
                     EYVNFNELVNFVLDRFDMMVGNQEKNYKIRREFTQRDLWVEVDTDKIIQVVDNIMNNA
                     IKYSPDGGTITCRLLETHNNVVLSITDQGLGIPKKDLNRVFERFYRVDKARARAQGGT
                     GLGLAISREVIKAHRGAIWAESKEGKGSTFYISLPYEPYEEEWWE"
     gene            4680..5996
                     /gene="yycH"
                     /locus_tag="ACG8KR_001879"
     CDS             4680..5996
                     /gene="yycH"
                     /locus_tag="ACG8KR_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system activity regulator YycH"
                     /protein_id="HFQ0201242.1"
                     /translation="MRISEKVVRIGLILMVALSIYFSYAIWLSPAGKSSINVDETNTQ
                     MIESQSNRKTSEVFLPLHVTWHHSGMIQETNSENLVYRLQLIIDKARFGKLSEVVSGD
                     KSQFEQVKNLNSGFELAYNAPFLLREYKEAFDLDLDVSNLAKESENMYFTRIQFDQGN
                     KKIRFLNYSNHLVYEANISLNWTDINKELTAKDTKWTEMTTEPSTIDTQYATKHSIKL
                     KKYSYILSQQPYTLFRNAFFQKPDSVKNNDESNELIFYGGNETMSIHSETQVVDFRGE
                     LPEKKENASVFSESFPYISKLGSSLGNLRYFDRSDNTIDYRIFVEGFPVFGTDSRGQI
                     GVEVGGETNGSVQVNIQTSLNTIQVPIPSDEEVELASTNEIIQQLKAKGADSNKIGSV
                     IVGYTWQNVKETNRVVDLLPEWYVKYKDNWYSANDLLEKLPGLEAN"
     gene            5997..6866
                     /locus_tag="ACG8KR_001880"
     CDS             5997..6866
                     /locus_tag="ACG8KR_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system regulatory protein YycI"
                     /protein_id="HFQ0201243.1"
                     /translation="MDFKKIEWIFFVAFLGLNVFLFSSYHDAVYQENNVIRSNQTESI
                     QQRLASDDITYTGKISSENKQGYYLSAEQTNLSTSLANYRKAGKSGLLASGVFLDGDV
                     LTKSLSSSDAEKNVIDPDKISSSLEDFLSNKDNILFGNDYVYTKNFSHLEEEPLEIQA
                     SQEYEGIPFNDDTAKIVLSLEKNDNDYSITKYMQTHLSDIEQLREKTELYTEEEAINT
                     LYINNKIPRGSKILWRQLAYSCILKVREKNVYVPVWHVAIETSDKVVQIESVNAFSNT
                     IVTNNTVPKVEDQ"
     gene            6921..7730
                     /locus_tag="ACG8KR_001881"
     CDS             6921..7730
                     /locus_tag="ACG8KR_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFQ0201244.1"
                     /translation="MAEEKPAFKISILASGSSGNSLYIESEKKRLLVDAGLSGKKITS
                     LMAQIDRTPDQLDGILVTHEHRDHIHGVGVLARKYHLDVFANQQTWEAMEPLIGNVPV
                     EQKHLFEMGKVRTFGDIDIESFGVSHDAAAPQFYRFHKENRSFVMLTDTGYCSDYMRG
                     VIENADGYLMESNHDLEMLRMGPYPWNLKQRILGDRGHLSNEDGALVMTDVIGNRTKR
                     IYLGHLSKENNMKELAHLTMENVLKEKDFGVGHDFEIYDTDPDEATELFSI"
     gene            complement(7833..8462)
                     /locus_tag="ACG8KR_001882"
     CDS             complement(7833..8462)
                     /locus_tag="ACG8KR_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HFQ0201245.1"
                     /translation="MKIALIGATGHAGSFILDEALSRDLDVTAIVRHPEKLPSDVPFI
                     QKDLFELTTTDLASFDVVVDAFNAPKGKEEMHQTSLSHLIEMLEGTDTRLIVVGGASS
                     LFLDTEKTTRMIDQVPKNAPFYPTAFNMYEGLLKLKQSKNLHWTYISPASLFDPHGKR
                     TGTYLLSDDYLQRNAAGDSTISMADYAIALVDEILDEKHEKQHISVVSR"
     gene            8632..9924
                     /locus_tag="ACG8KR_001883"
     CDS             8632..9924
                     /locus_tag="ACG8KR_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFQ0201246.1"
                     /translation="MKEWLDRAFLPMITSVVNLRPLTALRNGMTYTIPIIIVGSLFFL
                     FAHFPVDYIAQWIDKTGFSMYFTQVYQATFDVIAFWIVFAIAYAYAKEEDIDRLPVGI
                     TALSAYLLLIQPLNTQIDTVWTGAEGILGAVTTGLIIGWGYARLVRLAKGWKQREKIP
                     ENVWQSFTSLIPIIVIVTFTLIGSAVFQQLFRMTVVQGVWTYVQLPIQSFIDTLFGVL
                     LLGFFVPFLWLFGLHGSAMVNGLVSPVLQANSLANAEILASGQELTVANGGHIVTQQF
                     LDQFMTVTGAGLTLGAVFFMTVFAKSKKYRELGNLALLPAFFNINESIIFSTPIVMNP
                     MMAVPFIFAPVVSGLITYSALYFGFVPLFTAVQVPWTTPPILSGFLVGGVPAAILQGI
                     VLSISFFIYFPFLKKIDSANCKKERQTKNDGAAEKMND"
     gene            complement(10000..10662)
                     /locus_tag="ACG8KR_001884"
     CDS             complement(10000..10662)
                     /locus_tag="ACG8KR_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HFQ0201247.1"
                     /translation="MSLKKYSFLSIFCYGLIFISPLILASIGLVKSTSELITATAVMY
                     ILGAVVLAIFYFKQREPLAIESAVKKASAPKIVIYGLVGIFVALILQSIAVMLESVLF
                     GKATPSENTQNIIQMIMEAPAFILATTVAGPIMEEFVFRRSILGIISRYSNFWVGAVI
                     SSLLFAFAHNDGHLLIYFFLGFFFSLEYKATGRIWTSMITHVGMNTLVVLVQLAVQKG
                     LV"
     gene            10887..11171
                     /gene="groES"
                     /locus_tag="ACG8KR_001885"
     CDS             10887..11171
                     /gene="groES"
                     /locus_tag="ACG8KR_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HFQ0201248.1"
                     /translation="MLKPLGDRVIIEVAKEEEKTVGGIVLASAAKEKPQTGTVVAVGE
                     GRLLENGEKVPAAVKAGDQVMFEKYAGTEVKYEGKEYLIVAGKDIMAIVE"
     gene            11222..12847
                     /gene="groL"
                     /locus_tag="ACG8KR_001886"
     CDS             11222..12847
                     /gene="groL"
                     /locus_tag="ACG8KR_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736685.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HFQ0201249.1"
                     /translation="MAKELKFAEDARAAMLRGVDKLADTVKVTLGPKGRNVVLEKSYG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPLGIRRGIELATKAAVEELHNISTVVDSKEAIAQVAAVSSGSDKVGHLI
                     ADAMEKVGNDGVITIEESKGIETELDVVEGMQFDRGYLSQYMVTDNDKMEAVLENPYI
                     LITDKKISNIQDILPLLEQILQQSRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITDDLGLELKDVTIENLGNASKVVVDKDNTTIVEGS
                     GEKEAIEARVQLIKNQIAETTSDFDREKLQERLAKLAGGVAVVKVGAATETELKELKL
                     RIEDALNATRAAVEEGMVSGGGTALVNVISKVSAVEAEGDVATGIKIVVRALEEPIRQ
                     IAENAGYEGSVIVDKLKNVELGTGFNAATGEWVNMVEAGIVDPTKVTRSALQNAASVS
                     ALLLTTEAVVADKPEPAAPAAPAMDPSMMGGMM"
BASE COUNT         4374 a         2265 c         2832 g         3679 t
ORIGIN      
        1 aaataaaaaa agaacagcgt aaatgttttc gaaatgcttt cgcaccaaca tttacctgtt
       61 ccgtgggttt ggcacccaat gggccgtgag gggctcgaac ccgcgacccg ctgattaaga
      121 gtcagctgct ctaccaactg agctaacggc ccgtacaaaa agtacttaat aatagtagca
      181 ctaagaattt taaaatgcaa gactacattt aaagaaaatc agaaaaatct ttacttcctt
      241 ataggaaatc attcatagat tattcacatt tgctcgctat tatataagca taccaacaaa
      301 caaagttctt gcaagagaac aaaccctaac acttttttca ttttttactc ctttcccgcc
      361 gtctccccgg cgggtctcct gttcttaacg ataaaagaca agaaggctta gccttcttgt
      421 tttttgtttt catggaatcc cgctaatcaa atcacctagt taaagcaact agattgaatg
      481 tttatcataa gtctgatatc atgtttatga aatgaaaaaa ggagcggtta tgatggaaaa
      541 aatgcgtcag caattaatgg agtgggggaa ggaactagaa acattccgtc tgcctcactg
      601 ggaagaactc cctgacttag aactttatat ggaccaagtc agaacgttag ttgatcgtta
      661 tctttcacct gtgatacaag gagaaaagca tccattgctt acctcttcta tggtaaacaa
      721 ttacgttaaa ctaggtttga taccagcacc agttaaaaaa agatataaca aagaacacgt
      781 agcattttta cttgcaatca ctacgttgaa acaagtattg actattcctg aaatcaaaga
      841 agggatattg ttccaaggaa aaacagtagg aattcgtgaa gcctataatc tattttgcga
      901 tgaacaagaa gcagctgtgt ggatggtcag ccaattagca caaggtaaat ctcatccgca
      961 aaaattcgac caggcaaccc ctgtagaata tattgctgta agagcggcaa ctttatcgtt
     1021 tgcaatgaaa cttcttgcgg aaaaaacgat tgttctagaa accgaatatt taaaagagga
     1081 gaaaacaaat gaaaaacaat aaaatcgcaa ttttagtaga ttcaggtacg gatgtgccag
     1141 aagaattgat aaaaaaatac gagatgtata tgatcccatt gaaaattatt tataaagatc
     1201 aggtatatac agataaaatt gacattacct ctgaagagat ctatcaacgt ttgccagagg
     1261 aaatccctgg tacatctttg ccggatggtg agtcgatcac agaaatcttt gagcaaatca
     1321 aaaaagacgg ttatgaaaag gtacttgcgg taacaatttc cagtggatta agcgggacat
     1381 ataatgtggt tcgtttaatt gcacaacagc aagaagaatt ggaagcatat gtactagata
     1441 cgaaaaatat tggtattggt gcaggttttt ctgcgatcca agcagcaaaa tggctggaag
     1501 aaggagtaga atgggatcaa ctgatcagta atctgaatga gttagtcgaa agaacaaaag
     1561 ttttctttaa tgttgcgaca ttggaatatc ttcaaaaagg tggacggatc ggtttagttg
     1621 cttctattgt aggaaccgct ttaaagctta acccaatcat ctcatgtaat gaagaaggga
     1681 tttattacac agtaggaaaa gctaggggac gtaaaaaaag tttggataaa acggttgctt
     1741 acgtcaaaga aaaaatcggt aatgcaaagg tcttcaactt agctgttgcc catggggatg
     1801 cgaaagaaga agcaattgaa atggaagcga gacttaaaag agagttccca caagcaaacc
     1861 aaatctactt tggccagatt tctccggcat tagtagtcca cactggtccg ggattattgg
     1921 gtgtcggtgt acaagtttta gaaggataaa tcaaaaaaag gaaacactga ggtgaaaatc
     1981 agtgttttcc ttttttattg aagttagaac aaaaaataat aaagtatttt gcactttcat
     2041 tttaattttc tcttttgttt tataggcaaa gatgaaacaa aagcagaaat acggtataat
     2101 gagaaggtat gacaataata tataagaggt ggctctttat gaaaaaagtt ttagttgtcg
     2161 atgatgagaa gccgatttcg gatatcgtca aattcaattt agccaaagaa ggctatgatg
     2221 tttatactgc atatgatggg gaagaagcct tggaaaaagt tgctgaggta gaacctgatt
     2281 tgattttact tgatttgatg ctacctaaaa tggacggctt agaagttgct cgtgaggtac
     2341 gcaaaactta tgatatgccg atcattatgg taacagccaa agattctgaa attgataaag
     2401 tcctaggctt agagttaggc gccgatgatt acgtgaccaa gccattttcc aaccgagaat
     2461 tagttgcacg tgtcaaagca aatcttcgtc gcggtgccac ggctgcgaag gaaccagaag
     2521 aggcagcacc tgcggaattg acgattggtg atttgacgat ccatccagaa gcttacatgg
     2581 tgacgaaacg aggagaaaca atcgagttaa cacatcgtga attcgaacta ctcttttact
     2641 tggcgaagca ccttggacaa gtgatgacaa gagaacattt attacaaacg gtttggggtt
     2701 atgattattt tggagatgta cgaacagtgg atgtaactgt acgtcgtttg cgtgaaaaga
     2761 tcgaagataa tccaagccat ccaaattatt tagtgacacg tcgtggtgtc ggttactatt
     2821 tgcgtaaccc agaacaggag taattggatg aaaagaaaag tccgcttttt tcgatcagtc
     2881 aattttaaga tcgctattac ctttattctg attttattga tctcgatcga aattattggt
     2941 gcctacttta tacgtggttt agaacgttcc acgatcaata catttattaa agatatgaat
     3001 caaacggtgg aatcattagc aacaacaatc agcccggaat tgaatcgaaa agataatgca
     3061 gatgatgaag aggtaaatgc gaatatcaaa agatttattg aaaacagtgc gactagcgat
     3121 atcatcgaaa tacgggtcgt tgacgaaaaa gggatcatcc gaggaacaac cgatgtcaat
     3181 gaacaaagcg ctgtgggaaa gaaaaatgat tatgttgata tcaatgattt cacgactaaa
     3241 cgttatgttg ctttagataa cgacgataaa cgggtgaata tcaatgttca accgatcctg
     3301 tccccgaccg gcgatgccgt tatcggggct ctttacgtca aaagtaatat cgaacaaaaa
     3361 tattcggaaa tcaataacac agcagtcatt ttctttactg cctcactaat tgcggcattt
     3421 atttcaatga tcgtatctgt tttagttgct cgttcgatta cacagccgat cggtgaaatg
     3481 agagaacaag ccattcgaat cgctagagga gactacagtc ggaaagtcaa agtgcatgga
     3541 caagatgaac ttggacagct ggctgatacc ttcaatcagt tagctgaacg tattgaagaa
     3601 gctcaagata caatggaagc tgaacgcaat cgtttggata gcgtgctatc ccatatgaca
     3661 gacggcgtca ttgcgacaga tcgcaggggt aaagtcatta cgatcaatga tatggctgtt
     3721 tccttgctag atatcaaaaa cgaggaagca atcggacaat cgattttgac acttttagat
     3781 atcgaagaag aatatacgtt gcggaaatta ttagaagatc caaacgaaat ggtacttgac
     3841 cgttcaacca gtacattgga aagtgatcag atgatcttgc gagtagactt ttctatgatc
     3901 cgtcgcgaat caggttttat cagcggatta gtagctgtct tgcatgatgt gacagaacaa
     3961 gaaaaaacag aacgggaacg tcgtgaattc gtctctaatg tttcccatga acttcgtaca
     4021 ccgttgacaa gtatgcgtag ttatatcgaa gcattgagcg aaggtgcgtg gaaagacgag
     4081 gaagtagcac caaatttttt gaaggtaacg ctagatgaaa cagaccgaat gattcgaatg
     4141 atcaatgacc tcttaaatct atctcgcatg gatacaggta atacccagct tcaattagaa
     4201 tatgtcaatt tcaacgagtt agtgaatttt gttcttgacc ggtttgatat gatggtggga
     4261 aatcaggaaa aaaattataa gatccgtcgt gaatttacgc aaagagatct ttgggtcgaa
     4321 gtagatacag ataaaatcat ccaagtagtc gataatatca tgaataacgc gatcaagtat
     4381 tcaccagatg ggggaacgat tacttgtcgc ttattagaaa cgcataataa cgtggtttta
     4441 agtatcaccg atcaaggatt ggggattcct aaaaaagatc tgaaccgagt gttcgagcgg
     4501 ttctatcgag tagataaagc acgtgcaaga gcacaaggtg ggacaggact tggattagct
     4561 atttctagag aagtgattaa ggcgcatcga ggcgctattt gggcagagag caaggaagga
     4621 aaagggtcga ctttttatat ctcgctgcca tatgaacctt atgaggagga atggtgggaa
     4681 tgagaatctc tgaaaaagtc gttcgtatag gactcatttt gatggttgcg ttaagcatat
     4741 acttctctta tgcaatctgg ctgagcccag caggaaaaag ttcaatcaac gtagatgaga
     4801 caaacactca aatgatcgag tctcaaagca atcgaaaaac ttccgaagtc tttcttccgc
     4861 ttcatgttac atggcaccat tcgggaatga tccaagaaac caacagtgaa aatctcgtgt
     4921 accgattgca attgatcatc gataaagcaa gatttggaaa gctttctgaa gtagtttcag
     4981 gagacaaatc acagtttgaa caggtgaaaa atctgaactc tggttttgag ttggcctata
     5041 atgcgccttt tttgctaaga gaatacaagg aagcatttga tttagattta gatgtgtcga
     5101 acttggcaaa agaaagcgaa aatatgtatt ttactagaat acaattcgat caaggaaata
     5161 aaaaaatccg atttttgaat tattcgaacc atctcgtata tgaagcaaat atttcgttga
     5221 attggacaga tatcaataaa gaattaacag caaaagacac aaaatggaca gaaatgacaa
     5281 cagaaccatc gacgatcgac acgcaatatg ctacgaaaca ttcgatcaaa ctgaaaaagt
     5341 atagttatat cctgtcacag caaccctata cactttttag aaatgctttt ttccagaaac
     5401 cagatagcgt gaaaaataat gatgaaagca atgaattgat tttttacggt gggaatgaaa
     5461 caatgtctat ccattcggaa acgcaagtag ttgattttcg tggagagctg ccagagaaaa
     5521 aagaaaatgc tagtgtattt tctgaaagtt tcccctatat tagcaaatta ggcagttctt
     5581 tagggaacct cagatatttt gaccgcagtg acaatacgat cgattatcgg atattcgtgg
     5641 aaggcttccc ggtttttggg acagacagca gaggacagat tggtgtggaa gttggcggtg
     5701 aaacgaatgg ttctgtgcaa gtgaatatcc aaaccagttt gaatacgatt caagtgccga
     5761 taccttcaga cgaagaagta gaacttgcaa gtacgaatga aatcatccag cagttaaaag
     5821 ctaagggagc agatagcaac aagattggtt cagtgatcgt tggctataca tggcaaaatg
     5881 tcaaagaaac gaatcgtgtc gttgatctct tacctgaatg gtatgtgaaa tataaagata
     5941 actggtattc agctaacgac cttctggaaa aattacctgg attggaggcg aattaattgg
     6001 atttcaaaaa aatcgaatgg atatttttcg tcgctttttt agggttgaat gtgtttttat
     6061 tcagcagtta tcatgacgcg gtataccaag aaaacaatgt cattcgctct aatcagactg
     6121 aatcaatcca acaacgtctg gctagcgacg atatcactta cacggggaag atttcgtctg
     6181 agaacaagca aggctattat cttagcgcgg agcagaccaa tcttagcact tcattagcga
     6241 attatcgcaa ggctggtaaa agcggcttgc tggcaagtgg tgtgttttta gacggtgatg
     6301 ttttgacgaa atctttatcc agttcagatg ctgaaaagaa cgtgatcgat ccagataaaa
     6361 ttagtagttc cttggaagat tttttatcta acaaagacaa tattttattc ggtaatgatt
     6421 atgtgtacac gaaaaacttt tctcatttag aagaagaacc actagaaatc caagcaagcc
     6481 aagagtatga aggcatccca ttcaatgatg atacagcaaa aatcgttctt tctttagaaa
     6541 aaaacgataa tgattatagc atcacgaaat acatgcagac gcatttatct gatatcgagc
     6601 agctgcgcga aaagacggaa ctgtatacgg aagaggaagc aatcaataca ttgtacatca
     6661 ataacaaaat accaagagga tcaaaaattt tatggcgtca gctagcttat tcctgtattc
     6721 tgaaagtccg ggaaaaaaat gtgtatgttc ctgtttggca tgtagcgatc gaaacaagcg
     6781 ataaagtggt ccaaattgag agtgtcaacg ctttcagcaa tacgatcgtg acaaacaata
     6841 ctgtaccaaa agtggaagat caataaaaaa gcgagtataa tgaaaaaagt ttacaaagta
     6901 agaagggaag taggagtcaa atggctgaag aaaaaccagc ttttaaaatc agtatcctag
     6961 caagtggaag ttcgggtaat tcactttata tcgaaagtga aaaaaaacgg cttttggtcg
     7021 atgcaggact tagcggtaaa aaaatcactt ccttgatggc tcaaattgat cggacaccgg
     7081 accaattaga tggaatactt gtgacgcatg aacatcgaga tcatatacat ggcgtgggtg
     7141 tcttggctcg taagtatcat ttggatgttt ttgccaatca gcagacttgg gaagcgatgg
     7201 aaccactgat tggaaatgta ccagtggaac aaaaacatct atttgagatg gggaaagtca
     7261 gaacattcgg cgatatcgat attgagagtt tcggcgtctc ccatgacgca gcggctccgc
     7321 aattttatcg tttccataaa gaaaatcggt cattcgtcat gttgacggac accggatatt
     7381 gcagtgatta tatgcgaggt gtcattgaaa atgccgatgg ttatttgatg gaaagtaatc
     7441 atgatcttga gatgttacgc atgggacctt atccgtggaa tttaaaacag cgtattttag
     7501 gagatagagg acatctttcc aacgaagacg gtgcacttgt catgactgat gtgatcggta
     7561 atcggaccaa acgaatctat cttggccatc ttagcaagga aaacaatatg aaagaacttg
     7621 ctcatttaac aatggaaaat gttttgaaag aaaaagattt tggcgttgga catgattttg
     7681 aaatctatga tacagatcca gatgaagcaa ctgagctatt ttcgatttaa acagttcaat
     7741 caataaaaat ataaaaagca gaagataaaa tcaatcaggg ttggttttat cttctatttt
     7801 tattttttta gcatattaaa aaaataaccg tattatcgac tgactacact gatgtgctgc
     7861 ttttcatgtt tctcatccag tatctcatct actaatgcaa tcgcgtaatc tgccatgctg
     7921 atcgtactgt cgccagcagc gtttctttgt aaataatcat cacttaacaa ataagtacct
     7981 gtacgtttgc catgtggatc aaataaagaa gctggactga tataagtcca atgtagattt
     8041 ttagattgtt tcaattttaa caatccttcg tacatattga aagctgttgg atagaagggt
     8101 gcatttttag gtacttggtc aatcatacga gtcgtctttt ctgtatctaa gaataacgaa
     8161 gaggcacccc caacgacaat taagcgtgta tcggttcctt caagcatctc aatcaaatgg
     8221 gacaaagaag tttgatgcat ttcttctttt cctttgggag cgttgaaagc atcaacgaca
     8281 acatcaaaag atgcaagatc agtagttgtc aactcaaata aatccttttg tataaacgga
     8341 acatctgatg gcaatttttc tggatgacgc acgattgccg tcacgtctaa atctctggac
     8401 aatgcttcgt ctaaaataaa tgaacctgca tggccagtag ctccaatgag tgcaattttc
     8461 ataaaaaacc tcctttttta ttcctgtcca ttgtaagcag ttgctgaata ttcgtcaaag
     8521 aatccgattt ttgaaaagct cagtgcgcat tcctttcatg gttcgtgatc attgggtata
     8581 gttaaattaa gaaaactgat tgataaaaat agaagttgaa ggagataaga aatgaaagag
     8641 tggttagatc gtgccttttt accaatgata acatctgttg tgaatcttag acccttaact
     8701 gctttgcgta atggaatgac ttacacgatc ccaataatta ttgttggctc gctgtttttc
     8761 ttatttgctc attttcctgt ggattacatc gctcagtgga tagacaaaac aggattttct
     8821 atgtatttta cacaagtata ccaggctaca tttgatgtga tagccttttg gatcgttttt
     8881 gccattgcat acgcttatgc aaaagaagaa gacatcgatc gcttaccagt cggcataaca
     8941 gccttgtctg cttatctact gcttattcag ccactaaata ctcagataga tactgtctgg
     9001 acaggtgctg aagggatact aggagcagtt acaactgggc tcatcatcgg atggggctat
     9061 gcacgtttag taagacttgc gaaagggtgg aagcaaagag agaagatacc tgaaaatgtt
     9121 tggcagtctt ttacttcatt gattccaatc attgttatag tcacttttac gcttatcggt
     9181 tcagcggttt ttcaacagtt gttcagaatg actgttgttc aaggagtatg gacatatgtc
     9241 cagcttccca ttcaatcgtt catagacacg ttgtttggtg tgcttttatt aggttttttt
     9301 gttccgtttt tatggttatt tggtcttcat ggatctgcga tggtcaatgg gttggttagt
     9361 cccgtcttgc aagcgaattc attagcgaat gcagaaatct tagcctcagg acaagagttg
     9421 acggtggcaa atggcggaca tatcgttact cagcaatttc tcgatcagtt catgacggtt
     9481 acgggtgctg gattgacgtt aggggctgta ttttttatga cggtttttgc gaaatcgaag
     9541 aaatacagag aattaggaaa tcttgctctt ttaccagcct ttttcaacat caatgaatca
     9601 attatttttt ccacaccgat cgtgatgaac ccgatgatgg ctgttccttt tattttcgca
     9661 ccggtcgttt caggattgat tacctattcc gctttatatt ttggattcgt tcctttattt
     9721 acagcagtcc aagtcccttg gacaacaccg ccgatcctct cgggtttctt agtaggcgga
     9781 gtgcctgcag cgattttaca aggtatcgtg ttgtcaatta gttttttcat ttatttccca
     9841 tttttgaaaa aaatagattc tgcaaactgc aaaaaggaaa gacagacaaa aaatgatgga
     9901 gcagcagaaa agatgaacga ctaaaaaaca aaaatactcc tgtcgcttta gattgggaaa
     9961 atcaccaatc taggatgaca ggagtattta tagtcatgtt tatactagcc ctttttggac
    10021 agctaattga acaagaacaa ctaatgtgtt cattcctacg tgtgtgatca tcgatgtcca
    10081 gatccgacca gttgctttgt actctaggct gaagaagaaa cctaggaaaa agtagattag
    10141 cagatgacca tcattatgag caaacgcaaa cagcaaagag ctgatcactg ctccgaccca
    10201 aaaatttgaa tatcgactga tgatccccag tatactgcgg cggaatacga actcttccat
    10261 gatgggtcca gcaacagttg tcgccaaaat aaaagcgggt gcttccatga tcatctggat
    10321 gatattttgt gtattctcgg aaggggttgc tttgccaaac aatacagatt ctaacatgac
    10381 agcgatgctt tgcagaatca atgcgacaaa gattccaacc aatccataaa taacaatctt
    10441 tggagcagaa gcttttttta ccgctgattc gatcgctaag ggttcacgtt gcttaaaata
    10501 aaaaatagct aggacaacgg cgcctagtat atacatgact gcagttgctg tgatcaattc
    10561 agacgtagat tttacaagtc ctatcgaagc taggatcaag ggtgagataa aaattagacc
    10621 atagcaaaaa atgctgagga agctatattt ttttaaagac atgtaattcc tccttttaag
    10681 ggataacaag catgcaaggc aagcttatat cgattgattt caattatatc ataatttcaa
    10741 taagcagctg tcagaataaa atttcttttc tttgatttaa aagcttgcaa aaaagaggga
    10801 gaatgagtat tattaaaaat gtaggttagc actcgaaggt taagagtgct aacagattaa
    10861 aaaaagagtt atcggaggga tttatcgtgt taaaaccatt aggtgatcgc gtcattatcg
    10921 aagtcgcaaa agaagaagaa aaaactgtcg gaggaatcgt tttagcttct gctgctaaag
    10981 agaaacccca aactgggaca gttgttgcag tcggtgaagg ccgtttgtta gaaaacggtg
    11041 aaaaagttcc tgccgctgta aaagcaggcg accaagttat gttcgaaaaa tacgccggta
    11101 cagaagtaaa atacgaagga aaagaatatc tgatcgtcgc aggaaaagat atcatggcga
    11161 ttgttgagta aatttggaag ataaaaaaat aatcaaaaat aatcaatgag gtgaatcaat
    11221 catggcaaaa gaattgaaat ttgcagaaga cgcacgcgca gctatgttac gcggggtaga
    11281 taaattagca gatacagtga aagtaacatt aggacctaaa ggccgaaatg tcgtactaga
    11341 aaaatcttac ggctctccat tgatcacaaa tgatggtgtg acgatcgcaa aagaaatcga
    11401 attagaagat cattttgaaa acatgggtgc aaaacttgta tcagaagtag catcaaaaac
    11461 aaatgatatt gcaggagacg gtacaacgac agcaactgtt ctgacacaag caatcgttcg
    11521 cgaaggttta aaaaacgtaa ctgcaggtgc taatcctcta ggcattcgtc gcgggatcga
    11581 attggcaaca aaagcagcag tcgaagaatt gcataatatc tcaactgttg ttgattcaaa
    11641 agaagccatt gcacaagtag cagctgtttc ttcgggttct gataaagttg gacatttgat
    11701 tgctgatgca atggaaaaag ttggaaacga cggtgtcatc acaatcgaag aatcaaaagg
    11761 gatcgaaaca gaactagatg ttgtagaagg tatgcagttt gatcgtggtt acttatcaca
    11821 atatatggta acagataacg acaaaatgga agctgttcta gaaaacccat atatcttgat
    11881 cacagacaaa aaaatctcta acatccaaga tattctgcca ttgttagaac aaatcttgca
    11941 acaatcacgt ccattgttga ttattgctga cgatgtagat ggtgaagcat tgccaactct
    12001 tgtattgaac aaaatccgtg gaacattcaa cgtagtagct gtaaaagctc ctggtttcgg
    12061 cgatcgtcgt aaagcaatgc ttgaagatat cgctattttg acaggtggta cagtaattac
    12121 tgacgatcta ggtttagaat tgaaagatgt aacaatcgaa aacttaggaa atgcttctaa
    12181 agttgttgta gataaagaca atacaacgat cgtagaaggt tctggagaaa aagaagcaat
    12241 cgaagctcgt gtgcaattga tcaaaaacca aattgctgaa acaacttctg attttgaccg
    12301 cgaaaaatta caagaacgct tagctaaatt agcaggtggt gtagctgttg ttaaagttgg
    12361 tgcagctact gaaacagaat tgaaagaatt aaaattacgc attgaagatg cattgaatgc
    12421 tacacgtgcc gcagtagaag aaggaatggt ctcaggtggt ggtacagctc tagtgaacgt
    12481 tatcagcaaa gtttctgctg tagaagcaga aggagacgtg gcaacaggta tcaagatcgt
    12541 tgttcgtgct ttagaagaac caattcgtca aatcgctgaa aacgctggtt atgaaggatc
    12601 agtgattgtt gataaattga agaacgtgga actaggcact ggtttcaatg ctgctactgg
    12661 agaatgggta aacatggttg aagcaggtat cgtagaccca actaaagtaa cacgttctgc
    12721 cttgcaaaat gccgcttccg tttctgcctt gttattaacg acagaagcag tcgttgcaga
    12781 taaaccagaa ccagcagcgc cagcagctcc tgcaatggat ccatcaatga tgggcggtat
    12841 gatgtaagaa aaagcaactt gcttatctac aactgatcat tagtgaaaga cagataaaaa
    12901 gaggctggaa cagaagcgtt taactccaag aaataagaag aaattcacgg aaattgcttt
    12961 tcaaattttt gtgaatttca gcttatttcc gaaggagttg cttctgcttc cgctgtttat
    13021 acgtgtttag agcgtgagac aaaagtgttc tttacttttg tcccacgctc ttttttgtgt
    13081 gtaattataa tgaaaatagt tatttttaat agttataata ttttatctat taaaagtaaa
    13141 tatccattca
//
LOCUS       DAZKLD010000084        12917 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.084, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000084 DAZKLD010000000
VERSION     DAZKLD010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12917)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12917)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12917
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.084"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            137..580
                     /locus_tag="ACG8KR_001887"
     CDS             137..580
                     /locus_tag="ACG8KR_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFQ0201250.1"
                     /translation="MDKRQLLIEEYLDLANEIIHRNKPEMEKEFAGFTLNELEVIEHI
                     GKIPDPNVTKLANASYMTRGAISKLTKKLIARGIINTYQSEENKKEIYFQLTPIGQKI
                     RNRHEQLHQKWVQRDATVFENMSKEEFDTVFRFIGRYREFIRSSK"
     gene            complement(586..1770)
                     /locus_tag="ACG8KR_001888"
     CDS             complement(586..1770)
                     /locus_tag="ACG8KR_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance MFS efflux pump"
                     /protein_id="HFQ0201251.1"
                     /translation="MYSFTNKHSKVFGYVSVLFTGLGFTIVSPVLPFLTLPYSHSIHQ
                     QAFYITLLMSVYALAAFLSAPILGSLSDHFGRHPILIISLLGSSIGYLLFGLGNSIWM
                     LFLGRIIEGLTAGEISTLYAYFADITEPNERTKVFGWMGALVGIGTTLGPIIGGLLAE
                     LGNSVPIFIGALFTFLNAVYGYTFMLESLPIKKRSVDLSFSHVRPFHQLQQLFKISSV
                     IPLLTAGFAVWLAAGSLQSIFSQFSIDAFQWKAGLVGLSFSLIGILDTVSQLFIMPRL
                     LKKFSEQQITRIGMFSEILAYLFITLSGILLLPILFLFGIICYGFGDSIFTPVFNGQL
                     SNSVSDNQQGLVMGGTQSIQSLSRVIGPLIAGQLYAAAPFLPTVFGFLLMIFAYYHYN
                     KK"
     gene            2038..2736
                     /locus_tag="ACG8KR_001889"
     CDS             2038..2736
                     /locus_tag="ACG8KR_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HFQ0201252.1"
                     /translation="MLNLENYLSAGQTVISNLLLKNYHRIGLQSEEFLFVLQLHMAQL
                     EGDTFPDLQMIAMDMGIKQDQIFQILDRLVTNGFIKIETTIVNGKKADRYNLYPIYDL
                     LGEYLKTQEKKYEEKTQEKEIQSVYQLFEQEFGRPLSSIEFQRIGQWLEEDHYQPEIL
                     KLALREAVLNQAYSFNYVDRILLSWERKNLRTKQQVEEDQKRRKQQLLQREVEQSAQQ
                     EELPKVSLKNWLEE"
     gene            2743..3420
                     /gene="nth"
                     /locus_tag="ACG8KR_001890"
     CDS             2743..3420
                     /gene="nth"
                     /locus_tag="ACG8KR_001890"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HFQ0201253.1"
                     /translation="MLSKQKTMEALETMYGMFPEAHGELKHNNPFELLIAVILSAQAT
                     DVSVNKATPDLFASFPTPDALAEASIDEIILKIKTIGLYRNKAKNIKACAQQLIERFD
                     GQVPTSREELMSLPGVGRKTANVVLGDAFGIPAIAVDTHVERVSKRLRICKLDATVME
                     VEETLMRKVPQELWVKTHHTLIFFGRYHCTARNPKCEVCPLLSICQDGKNRMRLKEKA
                     LKKKPRL"
     gene            complement(3546..4079)
                     /locus_tag="ACG8KR_001891"
     CDS             complement(3546..4079)
                     /locus_tag="ACG8KR_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0201254.1"
                     /translation="MVLNNFLKEGFILSYLIVCLIIIICLHHLFLSKTIPRYSKNKKA
                     EKFCLLLNKFTLIAPILAFIIFSVLLSTTLKGKFMERSSHAMILTFLWLLFTRIYIFL
                     MSLKPPKSISLCLVINGIFLLSLIIFITPLDRYVTYLYNPLEYWTYFIGILEGIIFYI
                     GYFPNKNNNFYFYRNKL"
     gene            complement(4376..6757)
                     /locus_tag="ACG8KR_001892"
     CDS             complement(4376..6757)
                     /locus_tag="ACG8KR_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HFQ0201255.1"
                     /translation="MANEQTRSSRRQKQPTPKKSVKKNSGKGSGKSSGTHKKGLFIKI
                     LLGILSFFCILFLAGVGLFWYYAKDAPELTDKKLDATVSSKLYTQDGELFEDLGAEKR
                     EKISANELPKTLEDAIVSVEDRRFYKHIGVDPIRIIGSALSNFTSGGLQGGSTLTQQL
                     IKLSFFSTSAEDQTLKRKAQEAWMAVRLEQKKSKQEILTYYVNKVYMSNGLYGMETAS
                     EMYFGKKLSELSLPQTALLAGMPQAPSAYDPYVYPDQAKKRRDTVLYTMLQNEKISQT
                     EYDQAVNVPVTDGLQELTQSDDNTKIVDNYVKEVINEVQEKTDKNVYTDGLEIYTNLD
                     LDAQKKLYDIVNTDQYVSYPDDEMQVASTLIDTNTGKVKAQIGGRHIAEDVTLGNNLA
                     VNTSRDFGSTMKPVTDYGPAFEYLKYSTGKTITDAPYNYEGTSTPVGNWDNQYMGTIT
                     LRQALYLSRNVPAVKLFNEVGSDKVASFLKNLGIEYSTIHQSNAISSNTEEQDGTKYG
                     ASSLKMAAAYAAFANGGTYYKPQYVNKIVFQDGTEETYEPDGKTAMSPETAYMITDIL
                     KDTITEGTGTNAQIAGLYQAGKTGTSNYTDDEYAKLGISSGVYPDILFAGYTPNYSIS
                     VWTGYNKKMTPVTSESSHVASDVYRELMQYVSANVTNTDWEMPSGLIRVGGELYYKDQ
                     YTARSNAITPSTTIPSSSYVQTPGSSTTETTTQSSSSTSQSESTAESSKESTTAETSE
                     PASSTTVPSSSSEESSTPSSSAPPASSSEPATSGADAANDHTPSSSTSASGNR"
     gene            complement(6747..7394)
                     /gene="recU"
                     /locus_tag="ACG8KR_001893"
     CDS             complement(6747..7394)
                     /gene="recU"
                     /locus_tag="ACG8KR_001893"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HFQ0201256.1"
                     /translation="MVLRYPNGQPYYKNASPVEKIQEKRNQITEFGNRGMRFEEAINE
                     SNKYYLSRGLAVIHKKPTPIQIVKVDYPKRSAAVIKEAYFTQASTTDYNGVYRGFYLD
                     FEAKETKNKTSFPFKNFHAHQIEHMKACLEQNGVCFVLLWFSSLKRCFYLNSVHLIAY
                     WDAQKEKGRKSLPLSLIEELGIEIKTGISPRIPYLDAVDFYLSTLSIEGESKNGK"
     gene            7458..7997
                     /locus_tag="ACG8KR_001894"
     CDS             7458..7997
                     /locus_tag="ACG8KR_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HFQ0201257.1"
                     /translation="MDKSKVIYVSGYRSFELGVFKENDPKIQVVKNVLKKELHQLAEE
                     GLEWVLVSGNLGVELWAVQIVAELKEEYPELSLGILYPFSDFGNNWNEGNQEKKQLAE
                     QLADYVEAVSHQPYQSPSQLKNHTRFILEHTVGCLLIYDEEFPGKTKYFFEDAQNYQA
                     DHPYEIRLISMDDLQNFSE"
     gene            8087..8506
                     /gene="gpsB"
                     /locus_tag="ACG8KR_001895"
     CDS             8087..8506
                     /gene="gpsB"
                     /locus_tag="ACG8KR_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HFQ0201258.1"
                     /translation="MANLVYSPKDILQQEFKTKMRGYDPVEVDEFLDNIIKDYETYSK
                     ELLALQEENDRLSAKVAQLSKTQGAAQTRVQQTEAPKSAAVTNFDILKRLSNLEREVF
                     GKKLDQQASAVKPAQPNPNNYTNADTSLDDNEKTRQF"
     gene            8528..8895
                     /gene="rnpB"
                     /locus_tag="ACG8KR_001896"
     ncRNA           8528..8895
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="ACG8KR_001896"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            9104..10270
                     /locus_tag="ACG8KR_001897"
     CDS             9104..10270
                     /locus_tag="ACG8KR_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HFQ0201259.1"
                     /translation="MEKNRKFNLVATAASGLEALVGKELRDLGIECQVENGRARFEGT
                     METIATANLWLRTADRVKIVVGEFDAYTFDELFENVKALPWEDFLPLDAAFPVAGKSI
                     KSKLYSTPDCQAITKKAIVERLRTYYHRPASVPLTETGAHFQLEVALLKDHVMVTLDT
                     TGPSLFKRGYRLEKGGAPLKENMAAALIMLTNWRKDRPFYDPVCGSGTLCIEAALIGH
                     NIAPGFNREFACESWDWFSQEIMENVRSAAEEKADYDIELDITGSDINGRMIEIAKAN
                     AEEIGLGSSITFKQQAVKDFKTDKEYGVIVANPPYGERLGEEETVRKLYKEMGDVFRP
                     LKTWSKYILTSDLAFEEFYGQKATKKRKLYNGALRTDLFQYWGTRPPRKKEKDE"
     gene            10316..11812
                     /locus_tag="ACG8KR_001898"
     CDS             10316..11812
                     /locus_tag="ACG8KR_001898"
                     /EC_number="3.4.17.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HFQ0201260.1"
                     /translation="MKEKEFLQEVKEINLLQQALGILDWDTQTGMPEKASAYRSEVDS
                     YLYSIYFSKKIGPKIKEAIRYFSEHPEELSEVGTAVFAKVKEEYDLEHRVPEDLMRKY
                     TAAVSSAHGQWQKARETQQFSDFQHALNQNIELTKQLIPYWRKDEKTNYDVLLNQYEP
                     GMTVEILDKVFDQLKEGILSIRRTLQEKGTEPETDFLNRRMTKKQQKRFVVKVIEQLG
                     YDFSRGRLDDTVHPFMTGINPNDARITTRWNETDFKMAVFGVIHEAGHGMYEQDIDDK
                     YAYTPVYEGTSMGIHESQSLFNEIIIGSSRSFWKKQYPFFQECAEGTFDDIPFDKFYR
                     LLNHTKSSLVRIEADSLTYPLHIIIRYEIEKMIFNEGVDVAELPEIWNQKYEEYLGIR
                     PTNDVEGILQDVHWSGASFGYFPSYALGYMYAAQLLHSLQQEIDVENVLASDDYSPIK
                     NWMSEHIHQYGASRKPNQLIMDATHEPLNPQYLIDYMKDLYFNVYQVK"
     gene            11893..12576
                     /locus_tag="ACG8KR_001899"
     CDS             11893..12576
                     /locus_tag="ACG8KR_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFQ0201261.1"
                     /translation="MDKLQVIADYSMDQLAHDQTGHGSDHTKRVVKLAEKILETEPQA
                     DRFVTLAAAYLHDTIDDKVVKNENEAKQQLRVFLRSLPIVEEQISMIFSIIENMSFSK
                     NLSEAAELSLEGKIVQDADRIEALGAIGILRTAYFGGGHGHPIFDPELYPQTFKDKEN
                     YRKGTTVINHFYEKLFLLSDKMNTDYGYEEAKRRETFMREFLEEFFLEWSIDDENFTP
                     DNWIKNNKK"
BASE COUNT         4338 a         2250 c         2663 g         3666 t
ORIGIN      
        1 agaaaaaggg gttatgacaa gctttgtcaa cccctttttt tcttggtttc agattgggtt
       61 ttcctacaaa atatttttat ctttgttgaa taggaaacaa aaaaacaata taatgaaaga
      121 aaaagaaggt gtagaaatgg ataaaagaca actgctaata gaagaatatc tggatttagc
      181 taatgagatt attcatcgta ataaaccaga aatggaaaaa gaatttgcag gatttactct
      241 aaatgaacta gaagtgattg aacatatcgg gaaaatccct gatcctaacg tgacaaaatt
      301 agcgaatgct agttacatga ctagaggagc catcagtaag ctaacaaaaa aactgatagc
      361 tagagggatc ataaacactt atcaaagcga agagaataaa aaagaaatct attttcaatt
      421 gacgccaatc ggacaaaaaa taaggaatag acacgagcaa ctccatcaaa aatgggtaca
      481 gcgagatgct actgtatttg aaaatatgtc aaaagaagaa tttgacacag tatttcgttt
      541 tatcggtcga tacagagaat ttatacgaag tagcaaatga agctactatt ttttattgta
      601 gtgataatag gcaaaaatca taagcaaaaa tccgaatacc gtcggaagaa atggtgctgc
      661 agcataaagc tgtcctgcga ttagcgggcc gatgacgcgc gatagagatt gtatactttg
      721 ggtaccgccc attacaagac cttgctggtt atctgagaca gaattggata gttggccatt
      781 gaatacagga gtaaaaatag aatcaccgaa accataacag ataatcccaa ataaaaacaa
      841 aatcggcagt agaagaattc cagataatgt gatgaataga taggcgagga tttcactgaa
      901 catccctatt cttgtgatct gctgttctga aaattttttg agcaatcgag gcataatgaa
      961 caattgcgag acagtatcca agattccaat caaagagaag cttaaaccaa ccaacccagc
     1021 cttccactga aaggcgtcaa tcgaaaattg agaaaagata gattgaagag agccagctgc
     1081 caaccaaaca gcaaaacctg ctgtcaataa aggaataacg gatgaaatct tgaataattg
     1141 ttgtaattgg tgaaaaggtc ttacatggga gaaacttaaa tctactgaac gtttttttat
     1201 tggaaggctt tctagcataa atgtgtagcc ataaacggca tttaagaaag taaagagtgc
     1261 tccaataaat ataggaacag aattccccaa ctcagctaat agacccccta taataggacc
     1321 cagcgttgtt cctataccga ctaaagcacc catccatcca aaaactttgg ttctttcatt
     1381 aggttccgtg atgtcggcaa agtaagcata taaggtggag atttctcctg ccgtcaatcc
     1441 ttcaatgata cgaccaagga acaacatcca tatcgaattt cctaaaccaa aaagcaaata
     1501 tccgatactt gatcctaata aactgataat aaggatcgga tgacgcccaa aatgatcact
     1561 taagctaccg agaatggggg cagataagaa ggcagctaga gcataaacag acatgagcaa
     1621 agtgatatag aaagcttgct gatgtatgga gtgagaataa ggcagcgtta aaaacggaag
     1681 tacaggactg acgatagtaa atcctaatcc agtgaataag acagagacgt atccaaaaac
     1741 tttagaatgt ttatttgtga aactgtacat gtaaaaactc ctttatgcaa taatgttaac
     1801 ttggaaacaa aaatagtgta tagtaatttt gtttccttgt caacattcta aggaacagcg
     1861 aaatctactt aatttattta ttccgaaaag tgaagatacg catcaaaaac atgaacaaac
     1921 aaaaatcaag tagacgacat tgtaaatctg accgatcagt gaagtaaata gaaaagaaac
     1981 ctgttcttgc ttcccatgtt ttttttacgt atactgtgat tgagcggagg agaaatcatg
     2041 ttgaatttag aaaattactt aagcgctgga caaacagtca tatctaatct tttacttaaa
     2101 aattatcata ggatcggtct acaatctgaa gaatttttat ttgttctaca gttgcatatg
     2161 gcacagcttg aaggagatac tttccctgat ttgcagatga ttgctatgga tatgggaatc
     2221 aaacaagatc agatttttca aatactggat cgtctcgtaa caaatggatt tatcaagata
     2281 gaaacgacga ttgtcaacgg taaaaaagca gatcgatata atctttaccc catctatgat
     2341 ctattaggtg agtatttaaa aacacaagaa aaaaaatacg aagaaaaaac acaagaaaaa
     2401 gaaatccagt cagtgtatca attatttgaa caagaatttg gacgcccgct ttcttcgatc
     2461 gagttccagc ggatcggaca atggctagaa gaagaccatt atcagccgga aattttgaag
     2521 ctagcactgc gggaagccgt attaaatcag gcttatagtt ttaattacgt tgaccgcatt
     2581 ttgctatcgt gggaaagaaa aaatctacgt acaaaacaac aagtggaaga agaccaaaaa
     2641 cgaagaaaac agcagcttct tcaaagagaa gtagaacagt cagctcaaca agaagaattg
     2701 ccgaaagtct cactgaaaaa ttggttggag gagtagcgga atatgttaag caaacaaaaa
     2761 acaatggaag ctctagaaac gatgtatgga atgtttcccg aagcacatgg agaactcaag
     2821 cacaataatc catttgagtt attgattgca gtgatcttaa gtgcccaggc gacagatgta
     2881 tcagtgaata aagcaacgcc agatttgttt gcctcttttc cgacacccga tgcgttagca
     2941 gaagcttcaa tcgatgaaat cattcttaaa ataaaaacga ttggtttgta tcgaaataaa
     3001 gcaaaaaaca tcaaagcgtg tgcacagcag ctgattgaac gtttcgatgg gcaagtgcca
     3061 acgtcaagag aagaattgat gtctcttcct ggtgtaggca gaaagacagc aaatgtcgta
     3121 ttgggggatg cattcgggat accagcaatc gcagtagata cccacgtgga acgtgtctca
     3181 aaacgcctga ggatctgcaa actagatgct acggtcatgg aagtagaaga aacactgatg
     3241 cggaaagtac cacaagaatt atgggtaaag acacatcata cactgatttt ttttggtaga
     3301 tatcattgca cggcaagaaa tcccaagtgc gaggtatgtc ctttgctatc aatatgtcaa
     3361 gatgggaaaa atcggatgcg tctaaaagaa aaggcgctaa aaaagaaacc acgcctgtag
     3421 acgtggtttc tttaattgca aataaatgta tgaattatct tgttattatg gagaaaagct
     3481 gtttcgttta tcttttttgc atgaaaaagt aaatgtacat actcagtagc taggatggta
     3541 acttattaca atttattgcg ataaaaataa aagttgttat ttttattggg aaaatacccg
     3601 atgtagaaaa taatgccttc caatatacct ataaaataag tccagtattc caaaggatta
     3661 tataaataag tgacgtatct atctaaaggt gtaataaaaa tgataagtga taataaaaaa
     3721 ataccattta taactaaaca taaactaatg gatttgggcg gtttcaggct catcagaaaa
     3781 atatagattc tggtgaacaa caaccataaa aaagtgagaa tcattgcatg agagcttctt
     3841 tccataaatt ttcctttaag agtagtgctt aaaagtacag aaaaaataat aaatgcaagg
     3901 ataggagcaa ttaaagtaaa tttatttaat aataaacaaa atttttcagc ttttttattc
     3961 ttggaataac gaggtattgt tttcgataaa aaaagatggt gcagacaaat tatgataatt
     4021 aagcatacta tcagataact caaaatgaag ccttctttca aaaaattatt caaaaccata
     4081 tattgtattt tttatttgat aataacaatg ggattattaa gaagtggcac taaataaaag
     4141 actagtgtgt atttagccga tttaattata aacaagtaaa tggataaagt atatgtaatg
     4201 ataaaagaaa aatggatttt ctaattttta agctaaatgt tagatcacac atgtatatgc
     4261 tggatagtct tataaaaaat catgtaatac agcttggtac atgattttta tttgtagctg
     4321 ttttttacat agacacaaaa aagaaaccac gcttgtagac gtggtttctt ttttactatc
     4381 tgtttccaga agctgaggtg ctgctggatg gtgtatggtc atttgcagca tccgctccgc
     4441 tagttgctgg ctcactggaa gaagcaggag gagcagatga agacggtgtc gagctttctt
     4501 cggatgaaga agaaggaacc gttgttgaag acgctggctc gcttgtttct gctgtcgtgc
     4561 tttcttttga ggactcagca gttgattcgc tttgactagt agagctagag ctttgagtcg
     4621 ttgtctctgt tgttgagctt ccaggagtct ggacatagct ggaagatgga atagttgttg
     4681 atggtgtaat tgcattcgat ctagctgtat attgatcttt gtagtacagt tcaccgccca
     4741 cacgaattaa tccgcttggc atctcccagt ctgtatttgt cacgttagct gaaacatact
     4801 gcataagctc gcgataaaca tcagaggcta cgtgtgacga ttctgaagtg acaggtgtca
     4861 tttttttatt gtaacctgtc cacacggaaa tcgaataatt tggcgtatag cccgcaaata
     4921 ggatatctgg atacacccca gaagaaatac caagttttgc atactcgtca tctgtgtaat
     4981 tggatgttcc ggtcttaccg gcttgatata acccagcgat ttgcgcattc gtaccagttc
     5041 cttcagtgat cgtatctttt aaaatgtctg tgatcatata agctgtttct ggagacatgg
     5101 ctgtttttcc atctggctca taagtttctt ctgttccgtc ttggaagacg attttattaa
     5161 catattgagg tttgtaataa gtccctccat tggcgaaagc cgcataggct gctgccattt
     5221 ttaaggaaga agctccgtat tttgtcccat cttgttcttc cgtattacta gagatcgcat
     5281 tcgattgatg gatcgtactg tattcgatcc caagattttt taagaaagaa gccactttat
     5341 ctgaaccgac ttcattgaat aatttgactg ctggaacatt acgggataag taaagtgctt
     5401 ggcgtaaagt aatggtgccc atatattgat tatcccagtt tcctacagga gtcgaagtcc
     5461 cttcatagtt atacggtgcg tcagtaatag tttttccagt agaatatttc aaatattcaa
     5521 aagctggtcc gtaatctgtg acaggtttca tcgtagaacc aaaatcacgg gaagtattca
     5581 ctgctaagtt gttgcctaaa gttacgtctt ctgcgatgtg acgtccgcca atctgggctt
     5641 ttactttccc ggtattggtg tcgataaggg tggaggcaac ttgcatctcg tcgtctgggt
     5701 aagagacata ctgatccgtg ttgacgatat catatagttt cttttgggcg tccagatcca
     5761 aattcgtata aatctctaaa ccgtctgtgt agacattctt atctgttttt tcttgtactt
     5821 cattgatgac ttctttgaca taattatcga cgatcttagt attgtcatcg gattgagtta
     5881 gttcttgcag accatccgtt accggtacat taacagcttg atcatattct gtttgagaga
     5941 ttttttcgtt ttgaagcatc gtatacaaaa cagtatctcg tcttttcttc gcctgatctg
     6001 gataaacata aggatcatag gcggaagggg cttgaggcat cccagcaagc aatgctgttt
     6061 gcggtaaaga aagttctgat aatttcttac caaagtacat ctcagaagct gtctccatcc
     6121 cgtacaagcc gtttgacata tacactttat ttacgtagta ggtcaagatc tcttgtttcg
     6181 attttttttg ttccaatctt acagccatcc acgcttcttg ggccttgcgt ttcagagtct
     6241 gatcctcggc actggtggag aagaaggaaa gtttgatcaa ttgctgtgtc aaagtacttc
     6301 cgccttgcag tccgccagag gtaaagttag agagggcaga accgatgatc cgaatagggt
     6361 caaccccgat gtgtttataa aaacggcgat cttctaccga cacgatcgca tcttctaatg
     6421 ttttcggtaa ttcattcgca gaaatttttt cacgcttttc tgcgcctaaa tcttcgaata
     6481 gttcgccatc ttgtgtatat agtttggaag atacggtagc gtctaatttt ttatccgtca
     6541 attcgggcgc atcttttgcg taataccaga aaagtcctac acctgctaaa aagaggatac
     6601 agaaaaatga gagtattcct aaaaggatct tgataaataa tcctttttta tgtgttccag
     6661 aggactttcc agaacctttg ccagaattct tcttcacact ctttttagga gtaggctgtt
     6721 tctgtcgtct agagcttcta gtttgttcat ttgccattct tgctttctcc ttcaatagat
     6781 aatgttgata aatagaagtc aaccgcgtcc aaataaggaa tccgcggaga aataccagtt
     6841 ttgatctcta ttcctagttc ttcgatcaaa gataaaggta gtgatttgcg tcctttttcc
     6901 ttttgggcgt cccagtaagc aatcaaatga acactattca aataaaaaca gcgcttcaaa
     6961 gacgaaaacc ataacaagac aaagcagacc ccattttgtt cgaggcatgc tttcatatgt
     7021 tcgatttgat gggcatggaa gtttttaaag ggaaaagatg ttttgttctt ggtttctttt
     7081 gcttcaaagt ccaagtaaaa gcctcgatag acgccattat agtctgtagt tgaggcttgg
     7141 gtaaagtagg cttccttgat cacagcagca cttctcttag gatagtctac tttcacgatt
     7201 tggatagggg taggcttttt atggataacc gccaacccgc gggaaagata atatttatta
     7261 ctttcattga ttgcttcttc aaagcgcatc ccccgattgc cgaattcagt gatctgattt
     7321 cttttttctt gaattttttc gacgggagag gcattcttat aataaggttg gccatttgga
     7381 tagcgcaata ccatctttaa tcacactcct aggccacatt atagcaaaaa agcggataga
     7441 aagaaaagga atctgagatg gataagtcaa aagtaatcta tgtgtctggg tatcgaagtt
     7501 tcgaactcgg tgtttttaaa gaaaatgatc ctaaaatcca agttgttaaa aatgttttaa
     7561 aaaaagaact ccatcagtta gcagaagaag ggctggaatg ggtacttgtc agtgggaatt
     7621 tgggcgtcga actatgggct gtgcaaatag tagcggaatt gaaagaagaa tatccggaac
     7681 tatctcttgg tattttgtat cctttttctg attttggtaa taactggaat gaagggaacc
     7741 aagaaaaaaa acaattagca gaacaattag cagattatgt cgaagctgtt agccatcaac
     7801 cctaccaatc acctagtcag ctaaagaatc acactcgttt tatacttgag catacagtgg
     7861 gctgtctgct gatctatgat gaagaattcc ctggaaaaac gaagtatttt tttgaagatg
     7921 cacagaatta ccaagcagat catccctacg aaattcggct gatcagtatg gacgatcttc
     7981 aaaattttag tgaataatcg atattgtgtt tgatttatca acttttttcc tatacaatga
     8041 aaaaggtaga aatgttatta cgtaaatgaa aactagaggt gtaaatatgg cgaatttggt
     8101 ttatagtcct aaagacattt tacaacaaga attcaaaaca aaaatgcgcg gctatgatcc
     8161 cgttgaagtt gatgaattct tagacaatat catcaaagat tatgaaactt acagcaaaga
     8221 actccttgct ttacaagaag aaaacgatcg attgagtgca aaagtagctc aattatctaa
     8281 aactcaagga gcagctcaaa cacgcgtgca acaaacagag gcaccaaaga gcgcagcagt
     8341 gaccaatttt gatatcttaa aacgcttgtc aaatcttgaa cgtgaagtat ttggtaaaaa
     8401 gcttgatcaa caagcaagtg cagtcaaacc agcacaacct aatccaaata actacacaaa
     8461 tgcagacaca agcttagatg ataatgaaaa aacacgtcaa ttttaatttt tgattaatga
     8521 atttgcggtt ttcgggtaat cgcggtctga cttgatcagg ctgaggaaag tccatgctcg
     8581 cacaggctgt gatgcctgta gtgttcgtgc ttagcgaaac cataagctaa ggtactcttt
     8641 ggagtaacgg caggaaaaca agctaaggct tcggctatgc ttaagtatcc ttgaaagtgc
     8701 cacagtgacg aagcggctgg ggaaacccat ccggtggaac gcggtaaacc cctcgagcga
     8761 gcaacccaaa caatggtagg ggcgcttttt gtctggaaga agaacaggac aaaaaggcag
     8821 tataactgca gatagatggt taccgctttg ttttttctcc ctggaaaagc aaagtacaga
     8881 acatggctta tagaaaaccg caaacatcag ggaattaggt cgtgacaaaa gtaatgcgtt
     8941 ataagaaaca agaaaaacat ttgaaagcta tttttcaata cttcagatga atcgttctta
     9001 ttacaaaaca aacttttgta tcaccggtta ttcggaattt gagtaaaagt gtggggtagg
     9061 actgatgtcc cacctccttt ttttataaat aaggagattg tgtatggaaa aaaacaggaa
     9121 gttcaacctc gtcgcaactg cggcaagcgg gctagaagca ctggttggta aagaattaag
     9181 agatcttgga atcgaatgcc aagttgagaa cggacgtgca cgttttgaag gaacaatgga
     9241 aacgatcgct acagctaatt tatggttacg tacagcagat cgagtaaaaa tcgtggttgg
     9301 ggaattcgat gcgtatacgt ttgatgaatt gtttgaaaac gtaaaagcat tgccttggga
     9361 agattttctt ccactagatg cagcttttcc tgtagctgga aaatcgatca agtcgaaact
     9421 atatagcaca cctgattgcc aagcgatcac aaaaaaagca atcgttgaac gtctccgtac
     9481 ctattatcac cgtccagctt ctgtaccact gacggaaact ggcgcacatt ttcagttgga
     9541 agtagctttg ctgaaagacc atgtgatggt aactctggat acgacaggac caagtttatt
     9601 caaacgtggt tatcgcttag aaaaaggtgg agcaccattg aaagaaaaca tggcagcagc
     9661 acttattatg ttgacaaatt ggcgaaaaga ccgtccattt tatgatcctg tatgtggttc
     9721 aggtacgctt tgtattgaag cagccttaat aggccataat atcgcaccag gattcaatcg
     9781 tgagtttgct tgcgaatctt gggattggtt cagtcaagaa atcatggaaa atgttcgatc
     9841 agcagccgaa gaaaaagcag attatgatat tgaattagat attactggtt cagatatcaa
     9901 tggacggatg attgaaattg ccaaagccaa tgcagaagaa atcggattag gctcttcgat
     9961 tacctttaag caacaagcag tcaaagactt caaaacggat aaagaatacg gggtcatcgt
    10021 ggctaatccg ccttacggag aacgtctagg agaagaagaa actgttcgga aattatataa
    10081 agaaatgggc gatgtattcc gtccattaaa aacatggagt aaatatatct tgactagcga
    10141 cttagcgttt gaggaatttt acggacaaaa agcaaccaaa aaaagaaaat tgtacaatgg
    10201 tgcattgcgt acggatctat tccagtattg gggaactcgt ccgcctagaa aaaaagagaa
    10261 agacgaataa tcttactaat tgattcattc aattggatgt ttggaggaat agggaatgaa
    10321 agaaaaagag tttttacaag aagtaaaaga aatcaatctt ctacaacaag ctttaggaat
    10381 cctcgattgg gatacacaaa caggtatgcc tgaaaaagct agtgcatacc gaagtgaagt
    10441 agacagctat ctttacagca tttatttttc aaaaaaaatc ggtccaaaaa tcaaagaagc
    10501 cattcgctat ttttctgaac atccggaaga attgtcggaa gttggaactg ccgtttttgc
    10561 caaagtaaaa gaagagtatg atttggaaca tcgcgtaccg gaagatctta tgagaaaata
    10621 tacagctgcc gtttcttcag cacacggaca atggcaaaaa gctagagaaa cacaacaatt
    10681 ttctgatttt caacacgcat tgaatcaaaa tattgaatta acaaaacagt tgatacctta
    10741 ctggagaaaa gatgaaaaga cgaattatga tgttttgctg aatcaatacg aacccgggat
    10801 gactgtcgaa atccttgata aggtatttga tcagcttaaa gaaggaattc tttcgatccg
    10861 ccgtacgctc caagaaaaag ggactgaacc tgaaacagac tttttaaatc ggagaatgac
    10921 gaaaaaacaa cagaagagat ttgtagtgaa agtaatcgaa caacttggtt atgattttag
    10981 tcgtggtcgt ttagatgaca ctgtccaccc atttatgact ggaattaatc cgaatgatgc
    11041 acgaatcacg acacgatgga acgaaacaga tttcaaaatg gctgtttttg gagtgatcca
    11101 cgaggctggt catggtatgt atgaacaaga catcgatgat aaatatgcgt atacaccggt
    11161 ttatgaaggt acatcaatgg gtatccatga atcacaatcg ttgtttaacg agatcattat
    11221 tggaagtagt cgcagcttct ggaaaaagca atatcctttc tttcaagaat gtgcagaagg
    11281 aacattcgat gatattcctt ttgacaaatt ttatcgttta ttgaaccata caaaatcaag
    11341 tttggttcgt atcgaggcag acagtttgac ttaccctctc catatcatca ttcgttatga
    11401 aatcgaaaaa atgattttca atgaaggggt agatgttgct gaactacctg aaatatggaa
    11461 tcaaaaatat gaagagtatc tgggaatacg accaacaaat gatgtggaag gcatcctaca
    11521 agacgttcat tggtcaggtg caagctttgg ttattttccc tcttatgcat tagggtacat
    11581 gtatgcggca caattacttc attctttgca gcaagaaata gatgtggaaa atgttctagc
    11641 gtcagacgat tattctccaa tcaaaaactg gatgtcagag catatccatc aatatggtgc
    11701 atctagaaaa ccaaatcaat taatcatgga tgcaacacat gaaccgctta atccgcaata
    11761 cctgatcgac tatatgaaag acctgtattt taatgtatat caggtaaaat aaaatacaat
    11821 ttacaaagat agctgaaatt catctaatct atagatgttt tcagctattt acttaagaaa
    11881 gaagtggaac gaatggataa attacaagtg atcgctgatt atagtatgga ccaattagcg
    11941 catgatcaaa caggacatgg gagcgatcat accaaaagag ttgtcaagtt agctgaaaaa
    12001 attttggaga cggaaccgca ggcagaccgc tttgtgacgc ttgcagcagc ttacctacat
    12061 gatacgattg atgataaagt cgttaaaaat gagaacgagg caaagcaaca gcttcgtgta
    12121 ttccttcgct ctttgccaat agtagaggaa cagatctcga tgattttttc cattatcgaa
    12181 aatatgtcct tttctaaaaa tctctctgaa gcggccgaac tatctctgga aggtaagatc
    12241 gttcaagacg ctgatcgtat cgaagcatta ggagcaatcg gcattttgcg tactgcttat
    12301 ttcggcggtg gacatgggca cccgattttt gatcctgaac tgtatccgca aacattcaag
    12361 gacaaagaaa attaccgaaa aggcacgaca gtgatcaatc atttttatga aaaactgttc
    12421 ttgctgtctg ataaaatgaa tactgattac ggatatgaag aagccaaacg aagagaaacc
    12481 ttcatgcgtg aattcttaga agaatttttt ctggaatggt ctattgacga tgaaaatttt
    12541 actccagaca attggatcaa aaataacaaa aaatgatgga ggttgtgaca gaagtggtca
    12601 gctccaagat atttcggatc attttccgta aaagttactt ctggaacact gtttaccaag
    12661 aggtcgtgag cctgccgttc agctccgagt cacaaggaac aatttcaaaa aatagcttct
    12721 cgtgtttgat gaatattgta acttgtgatt cggaattggc aggagaacac cgtttattcg
    12781 gaagaatagg actgtgacaa gacttttgtc acagtccttt tttcgaattc aacaagtaaa
    12841 acttttaaga aatattcaga gagtaaaatt aaaaattgct gataaacgaa gatggaaatt
    12901 ttatgtattt ttattgg
//
LOCUS       DAZKLD010000085        12776 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.085, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000085 DAZKLD010000000
VERSION     DAZKLD010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12776)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12776)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12776
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.085"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            115..2280
                     /gene="acm"
                     /locus_tag="ACG8KR_001900"
     CDS             115..2280
                     /gene="acm"
                     /locus_tag="ACG8KR_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Acm"
                     /protein_id="HFQ0201262.1"
                     /translation="MKKCRNIILSMLFIITNITSLIPVHVYADAGRDISSNVTSLTVD
                     PTNITDGGNIKVKFSFDEKKQNIQPGDYLWINWPSEGNIRGEGFQKEIPLMIENKNVG
                     TLTVRKDSAQVVFNENIKNLDSVEGWGQFEIQARNVTDTGEENTGPFTVTSGDKTATV
                     NVTKPASGSSSSVFYYKTGDMLPEDTKHIRWFLNINNNGTYVEQPVKISDEIQSGQRL
                     DPSTFEINQIHLGEQKVYRGEEGIQQFLQDFPSATFNFSVTDNYIEITIPKNFVNLRK
                     IMVSYKTIIENPEQINFENHSEAWFKEFNKPAVDGESFNHTVKNISASGGVNGTVRGE
                     LKIFKYINDTEIGIPNVTFELRRADEQPIQGQSSILLTSNEQGEASIKGLQVGDYVVK
                     EKEAPNWIDFDPLSSNELKFSINENDTEGVSLPIYNKKKVTNITATKIWNGGTTPRPS
                     IYFKLFRASTNNWEPVPDAETKRLDNGITSVTWEDIQQYDDSGNEYTFKVQEVDQNGN
                     DYVPSGYQKIENGLVVTNENKEVISVSGQKTWEDNENQDGKRPTTITVNLLADGQPIQ
                     HKEVSEKDDWRYRFTNLPKYRDGQEIIYTVTEDNVPEYSTTIDGYNIKNSYTPEKTNI
                     SIPGSHITPWKNFPEKTEREKIRNLFSQKLQIFAHTGESSEQRSNYISKRAVPKQITN
                     KSKSILPKTSSKKSSFAVIIGLLIVTICAGIFLQKYKKV"
     gene            complement(2552..3037)
                     /locus_tag="ACG8KR_001901"
     CDS             complement(2552..3037)
                     /locus_tag="ACG8KR_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HFQ0201263.1"
                     /translation="MSRATEQEAYELLKKLGISFQKVDHPAITSVKNVPFSLPGPQVK
                     NLLLKSRKGKQIYLVILPDEKHADLKQLAEQLKEKRLSFVSEEQLPDLLGVPAGTVTP
                     LALMHDKEKKIQVVIDAAIDRQDTVGFHPNINTTTLIMDFSDFEKILVYLEHLPVYEN
                     L"
     misc_binding    3127..3472
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            3517..4158
                     /locus_tag="ACG8KR_001902"
     CDS             3517..4158
                     /locus_tag="ACG8KR_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFQ0201264.1"
                     /translation="MEYLIEVMPQLLVGALTTLKLFLFTLIGSLPLGIVLALGLSLHF
                     KPLRYLLQTYVWVMRGTPLLLQLIFIFYGLPTIGIVFDRFEAALIAFILNYAAYFAEI
                     FRGGIQSIPQGQYEAAQVLQLSPFQTVTKIVIPQVTKIVLPSVGNEVINLVKDSSLIY
                     ILGLGDVMRAGKIAMERDATLLPLLGVAIIYLTLTGILTIVMKQVEGKLNYYR"
     gene            4177..4812
                     /locus_tag="ACG8KR_001903"
     CDS             4177..4812
                     /locus_tag="ACG8KR_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFQ0201265.1"
                     /translation="MIELKKVNKAFGEKKIIKDLNLIIPDGKTLAIVGPSGGGKTTLL
                     RILAGLESSDSGEFYLDGVPFNPFELKMKEQVVGVVFQDFQLFPHLNIFENITLAPKM
                     VLKQTKEEYTKKAEELAASLGISDLLNIYPYQLSGGQKQRVAIARALAMNPRVLAYDE
                     PTSALDPELRQQVERLILSLKEDGVTQIIVTHDLVFAENVGDQLLKVSPIH"
     gene            4830..5645
                     /locus_tag="ACG8KR_001904"
     CDS             4830..5645
                     /locus_tag="ACG8KR_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HFQ0201266.1"
                     /translation="MKKTSFLFALIAGLLLFAGCSNKKTSADNWATINEEKKIVIGLD
                     DTFVPMGFRDKDGKLTGFDIDLAKAVFEQYGITVDFQPIDWSMKEFELNNGTIDLIWN
                     GYSKTPAREKKVQFTKPYMENDQVLITPKESGITNFGQMNNKRLGAQNGSSGYDVFTK
                     QPEVLKEIVKNQDAVLYDSFNEALIDLKSRRVDGLLMDKVYADYYLKQRNELEDFNIT
                     KGSYESEDFAVGARKSDSTLVKKINEALDQLEKTGEFQKISDKWFGEDVTPRN"
     gene            5964..7400
                     /locus_tag="ACG8KR_001905"
     CDS             5964..7400
                     /locus_tag="ACG8KR_001905"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFQ0201267.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            7589..10036
                     /locus_tag="ACG8KR_001906"
     CDS             7589..10036
                     /locus_tag="ACG8KR_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HFQ0201268.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            10266..11333
                     /gene="rlmN"
                     /locus_tag="ACG8KR_001907"
     CDS             10266..11333
                     /gene="rlmN"
                     /locus_tag="ACG8KR_001907"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HFQ0201269.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            11323..11529
                     /locus_tag="ACG8KR_001908"
     CDS             11323..11529
                     /locus_tag="ACG8KR_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0201270.1"
                     /translation="MPNNRILLVFSITIKGAVFLIKELFNLNKRKDSIAKAIKLFLTR
                     KTLIKQRNVLNLMTACLMGVLAII"
     gene            complement(11526..12629)
                     /locus_tag="ACG8KR_001909"
     CDS             complement(11526..12629)
                     /locus_tag="ACG8KR_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HFQ0201271.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
BASE COUNT         4360 a         2138 c         2732 g         3546 t
ORIGIN      
        1 tatcaatttt ttagttaaaa gattggaaat gagtggggga ggaattgaag aaaggatttt
       61 tgagagatga tatagtaggt tttattttta gttaaaatag gggagataga aaaaatgaaa
      121 aagtgtagaa atattatttt atcaatgtta tttattatta ctaatataac atcactgatt
      181 cctgttcatg tctatgcgga tgcaggcaga gatatcagca gtaatgtcac ttcgttgacc
      241 gtggacccaa cgaatattac agatggagga aacataaaag taaaattctc gtttgacgaa
      301 aaaaaacaaa atatccaacc aggtgattat ctttggatca attggccaag tgaaggaaat
      361 attcgcggtg aaggattcca aaaagaaatc ccattaatga ttgaaaataa aaatgttgga
      421 actttaacgg tcagaaaaga ttctgcgcag gttgtgttta atgaaaatat taaaaaccta
      481 gactcagtag aaggttgggg gcaatttgag atacaggcca gaaacgtaac cgataccggt
      541 gaagaaaata cgggaccatt caccgtgacg agcggtgata aaacagctac tgtaaatgtg
      601 acaaagccag cttctggttc ttcttctagc gttttttatt ataaaacagg agacatgcta
      661 cctgaagata ctaagcacat tcgatggttt ttgaatatta acaataacgg cacttatgtt
      721 gaacaaccag ttaaaatcag tgatgaaatc caaagtggac aaagattaga tccaagtacg
      781 tttgaaatta atcaaataca cttaggtgaa cagaaggttt atagaggaga agagggaatc
      841 caacaatttt tacaagattt ccccagcgct acatttaatt tttcagtaac tgataattac
      901 attgaaataa ctataccaaa aaactttgtc aatcttagga aaataatggt gagttataag
      961 acgatcatag aaaacccaga acaaataaat tttgagaatc attcggaagc ttggtttaaa
     1021 gaatttaata aacctgctgt tgatggagaa agtttcaatc atacagttaa aaatatttca
     1081 gcttctggtg gagtcaacgg tacagttaga ggcgaattga agattttcaa atatatcaat
     1141 gacacagaga ttggtatccc aaatgtgact ttcgaattaa gacgagcaga tgaacagcct
     1201 attcaaggac agtctagtat cctgttgact tcgaatgaac agggagaagc tagtatcaaa
     1261 ggcctgcaag taggagacta tgttgtaaaa gaaaaagaag cacccaattg gattgatttt
     1321 gatccactaa gttcaaatga actcaaattc tccataaacg aaaatgatac agagggagtc
     1381 agtttaccta tttataacaa gaaaaaggtt acgaacatca cagcaactaa aatttggaac
     1441 ggtggaacca ctccaagacc gagtatttat tttaaattat tcagagcaag tacaaacaat
     1501 tgggaaccag ttcctgatgc agaaacaaaa cgattagaca atggaataac ttcagtgact
     1561 tgggaagata tccaacagta tgatgatagt ggaaacgaat atacgtttaa agtgcaagaa
     1621 gtagatcaaa acggaaatga ttatgtccct tcaggttatc aaaaaattga aaacgggcta
     1681 gtcgttacaa atgagaataa agaagtcatt tcagtctcag gacaaaaaac atgggaagac
     1741 aatgagaacc aagacggcaa acgaccgacg acgatcacag taaatctatt agccgacggt
     1801 caaccgatcc aacacaaaga agtaagtgaa aaagatgatt ggcgctatcg tttcacaaat
     1861 ctgccaaaat acagagacgg gcaagaaatc atctatactg taacggaaga caacgtgccc
     1921 gaatacagta cgacgattga cgggtataac atcaaaaaca gttatacacc ggaaaaaaca
     1981 aatatatcta taccaggtag ccatataaca ccgtggaaaa attttccaga gaagacagag
     2041 agagagaaaa ttagaaatct attctctcaa aaattgcaaa tatttgctca tacaggagaa
     2101 tccagcgaac aaagaagcaa ttatattagt aagagagctg tacctaaaca gataacaaat
     2161 aaatcaaaaa gcattcttcc taaaacaagt agtaaaaaat ctagttttgc tgtaatcatt
     2221 ggtttattga tagtaactat ctgtgctggg atattcttac aaaaatataa aaaagtttag
     2281 ttagaatata atattttttg ttaaatatga ctgaaatcaa aagaataaaa ttccgattta
     2341 taatcgaaaa aagaagcatt tttatatatg cttctttttt cgtagaatca ataaatatgg
     2401 atttggctaa ctatggaaga atactcgtat tagttttcag cgttcatact agttgttaca
     2461 ttgtttgatt ggccaaaaga atttctgata tattttgtgc taaataaaga aaaagacgct
     2521 tgaattggga aataagaaaa ctactctatc attacaaatt ttcataaact ggcagatgtt
     2581 ctaagtaaac aagtatcttt tcaaagtcgg aaaaatccat aatcaacgta gtggtattga
     2641 tatttggatg aaagcctacg gtatcttgac gatcaatagc agcatcgatc acgacttgga
     2701 tttttttctc tttgtcgtgc atcaatgcta atggagtcac tgtacctgct ggaacgccta
     2761 ggaggtcagg aagctgttct tcagaaacaa aagataagcg cttttctttt aattgttcag
     2821 ctagttgttt taaatcggcg tgcttttcat caggcaagat gacaagatag atttgttttc
     2881 cttttcttga tttcaataat agatttttta cttgcggacc aggtagggaa aaagggacat
     2941 ttttgactga cgtaatcgca ggatgatcga ctttttgaaa agagataccc agttttttta
     3001 gtagttcata agcttcttgt tcggttgcac gactcatcgc gcatcaccct ccttttttta
     3061 ttagtgtatc ataaaaaaga gttgacaaat gaaaaataat catgtaaatt acacgtaaag
     3121 ttaaaacaaa tgacatcgaa aagaagagta gccgttaggt gtttttagag agtctctggt
     3181 tggtggaaag aggcagcagt taaccgtgaa acacatcttg gagtcgattt tctgaagcgt
     3241 atagtaggaa aatccgggga gcccgttaca gctgcggtcg ttgctagatg accagataag
     3301 gctggctttt ccgtgaggag tcggtaaaaa aaggtggaac cacgtgatta acgtcctttg
     3361 atcttcggat caaaggactt tttgttttca tctgaacggc aacgactgtg tgaggttttt
     3421 tctgcaagga aaaaataaac agaggtggaa ccacgtataa tacgtcctct tggcatttgc
     3481 caagaggatt tttttatttt aaaggaggaa aagaagatgg agtatttgat tgaagttatg
     3541 ccgcaattat tagtaggagc actcacaaca ttgaaattat ttttatttac cttgattggc
     3601 tcattaccat tagggattgt tttagcatta ggattgtctt tgcattttaa gccgctacgc
     3661 tacttgcttc aaacatatgt atgggttatg cggggaacac cgctgttgtt gcaactgatt
     3721 tttatttttt atgggttgcc gacgattggg atcgtttttg atcgtttcga agcagcactc
     3781 attgccttta tcttaaacta tgcagcttat tttgcagaga tctttcgtgg agggatacaa
     3841 tcgattcctc aaggtcaata tgaagcagcg caagttttgc aattaagtcc ctttcaaacg
     3901 gtgacgaaaa tcgtcatccc acaagtgaca aagattgttt taccttctgt agggaatgaa
     3961 gttatcaact tggtcaaaga ttcttccttg atctatatat taggtttagg tgacgtaatg
     4021 cgtgctggga aaatcgcgat ggaacgcgac gcgactttac tgcctttgct aggcgtggca
     4081 atcatctatc ttacattgac tggtattcta acaatcgtaa tgaaacaagt tgaaggaaaa
     4141 ttaaattatt accgctaact gataggagga agaaatatga ttgaattgaa gaaagtgaac
     4201 aaagcatttg gtgaaaaaaa aataatcaaa gatttgaatc taatcattcc agatgggaaa
     4261 acattagcta tcgtaggtcc ttccggtggc ggtaaaacca cgcttttacg tatcttggct
     4321 ggcttagaaa gttctgacag cggagaattt tatttagacg gtgtaccgtt caacccattc
     4381 gagcttaaaa tgaaagaaca agttgtagga gtcgtattcc aagacttcca gctatttcca
     4441 catctgaata tttttgaaaa cattacctta gcaccaaaaa tggtattgaa acagacaaaa
     4501 gaagaataca ccaaaaaagc agaagaatta gcggcttcat tagggattag tgatttattg
     4561 aatatctatc cttatcagct ttctggcgga caaaaacagc gggtagcgat tgccagagca
     4621 ttggctatga atccacgggt attggcttat gatgaaccaa caagtgcttt agatcctgaa
     4681 ttacgccagc aagtcgagcg tttgatccta tcattgaaag aagatggggt aacccaaatt
     4741 attgtcaccc atgatttagt atttgcagaa aatgtcggtg atcaattact aaaagtttcc
     4801 ccaattcatt aagaaaggag aagatagaga tgaaaaaaac cagttttcta ttcgctttga
     4861 tcgcaggtct tcttcttttt gcaggctgtt cgaacaaaaa aacttcagca gataattggg
     4921 cgactatcaa cgaagagaaa aaaatcgtta taggtttaga cgatacattt gttcctatgg
     4981 gatttagaga taaagacggg aagctcacag gatttgatat tgatttagcc aaagcagtct
     5041 ttgaacaata tgggattacg gtagactttc agcccattga ctggtcaatg aaggaatttg
     5101 aactgaacaa tggaacgatc gatttgatat ggaacggcta ttccaaaacg ccggcaagag
     5161 aaaagaaagt gcaatttaca aaaccttata tggaaaacga tcaagtgctg atcacaccta
     5221 aagaatcagg aatcacaaac tttggacaaa tgaataataa gagattaggt gcgcaaaatg
     5281 ggtcatctgg ttatgatgta tttacgaaac aaccagaagt attaaaagag atagtcaaaa
     5341 accaagatgc tgttttgtat gatagtttta atgaagcatt gatcgatctg aaaagcagac
     5401 gggtcgatgg tctgctgatg gataaagtat acgcagacta ttatttgaaa caaagaaacg
     5461 aactagaaga cttcaacata acaaaaggtt cttatgagag tgaggatttt gcagttggtg
     5521 cacgcaagtc agatagtaca ttagtgaaaa aaatcaatga agccctagat caactagaga
     5581 aaacaggtga attccaaaaa atatccgata aatggtttgg cgaagatgtt acgccgagaa
     5641 attaacaaat aggggactgt gacaaaagac gtgtcacagt ccctttttcc gaataaacag
     5701 tgttctcctg ccaactcctt gtgactcgga gctgaatggc aggctcacaa cctcttggta
     5761 aacggtgttc aaaagccagt ttttcggtat taagccaaat ttatgcaaaa atgtagaaag
     5821 ccattttcac aaaatttttc ttactactcg aaactaagcg actttttcac aacctccttt
     5881 acatattttt attttgttgt ggaaggggtt tcgtgttatt ctaaaagagg aacgattgaa
     5941 ggttatagga aaggaagaga tgaatggaac acaaagaact tacctcattt ccaaaagatt
     6001 ttctgtgggg ctctgcttct gcagcgtatc aagtagaagg agcttggcaa gaagatggga
     6061 aaggagaatc agtctgggat cgatttgtac ggatacctgg aaagacattt aaaggaacga
     6121 acggtgattt ggcagtagac cactatcatc gttataaaga agatattgct ttaatgaaag
     6181 aacaaggtct gaaagcttac cgcttctctg tagcttggac acggattttt cctaatggac
     6241 gaggagaagt caatcaagct gggctcgctt tttatgagag attgatcgac gaattgattg
     6301 aaaacgatat tgaacctatg ttgactttat atcattggga tcttccacaa gcattgcaag
     6361 atgaatataa tggttgggaa tcacgtcaga tcattgaaga tttcactcat tatgcagaaa
     6421 cattatttga ggcattccgc gggaaagtcc attactggat tagtttgaat gaacaaaata
     6481 tcttcacttc attaggttat ttgttagcag cacatccgcc aggtgtgaca gacccgaaac
     6541 gaatgtatga agttaaccat attgctaatt tagcaaatgc atctgtcatc aataaatttc
     6601 acgagttaga aatgcctgga aaaatcggac caagctttgc gtatacgcca aattatccaa
     6661 ttgacagtaa tcctgaaaat gttctggctg ctgaaaatgc agaagacctg atggccaatt
     6721 attggatgga tgtctatatg tggggaaaat atccgattgc tgccatgagg ttcttagaag
     6781 aaaaaggatg ggcaccaacg attgaagctg gcgatgcaga attgctagaa tctgctaaac
     6841 cagattttct tgggatcaat tactatcaaa cggcgacgaa tgcattcaat ccattaaacg
     6901 gagttggtgc tggaaaaatg aatacgacag gaaaaaaagg ttcatcagaa gaaactggag
     6961 ttcctggaat gtataaaaaa gttgagaatc catttgtcga acgaacaaat tgggactggg
     7021 agattgaccc agaaggatta aggatcggac tgcgtcgtat cacaagccgt taccgcatgc
     7081 cggtactgat tacggaaaat ggcttaggag agtatgacaa gttgacagaa gataaacaaa
     7141 tccatgacga ctatcgaatc aattacttac aaagccacgt caaagccatc aaagaagcaa
     7201 tttcggatgg agcagaagtc ctaggctatt gcacctggtc ttatacagat ttgcttagct
     7261 ggctgaacgg ttatcaaaag cgatatggat ttgtatatgt tgatcaagac gaaacacaag
     7321 aaggatcttt ggaacgatat aagaaagaca gttattactg gtaccaaaaa ctgattgaag
     7381 aaaatggaga gaacctttag ttcacatact taaaacaaga aggtacggct ggatatggcc
     7441 gtgccttttc ttgataaaat catttagaaa ttaagaaaaa ctgtaaaata agctgaaaaa
     7501 tgaaagaaag cgtcagttct ttcaaacaaa actttagttc ccttactttt ttttgtataa
     7561 tgaaacttga tgaaatgagg gataataaat ggcacaaaag aaacggccta ctaaaaaaag
     7621 gaaaaagacg aaaaaaacga aaaagcaaaa acagcagcag gaacaaatgc tgacgattgg
     7681 tgttgggttt gccttgattt tatttgctgt cttcggcttt ttaaaattag gttttttagg
     7741 aatattgatt gcaaacggct ttcggatcat tgcaggaaat acttaccaaa tcctctgtct
     7801 tttattagcc gtattaggat tttggattgt cattaagaat acagagtttt caattggtaa
     7861 aaaccgacgc tggtttggcg gcatactctt ttatttcggt atcttattat tgttacatgc
     7921 ccatttattt ggcaaactcc atactgggga accaaatatc atggggacga catgggattt
     7981 gttagccagt gatataaaac aaagtcaagt agacaataac gttggtggtg ggatgattgg
     8041 agcaatcctt tatcatttta cttacttctt gattgcacag cctggaagtt atttagcagc
     8101 agtgctatta ctggcaggag gagcattcct gttcagtaat ctggaaggct atcagctgtt
     8161 aaatggtatc caatcaattg gagaacgagt acaggaatta ttggaaggtg atcctgccaa
     8221 gcaagctcga aagcaagcgg caaaggaaga gagaatgaaa cagcgcgcag aagcgaaaga
     8281 agcaaggcgc ttagctgcaa aagaagctgc agaaaaagaa gctgcagaat atgagaagaa
     8341 aaaagcggtg agccagcaaa gggaaaatac tgctgatgaa tggcaggaac cagaaaatac
     8401 agagccggag cagctaagtt ttgttccaat cgacagtttt caagaaaata tccatcctgc
     8461 gaatctagag aaaccagtac cagatacacc aaaacagacc aatacagcag aaggtttttc
     8521 agatgagttg ccagaggatg acggtacctc attagaattt gagattgaag cagaacagga
     8581 aaatcaagac tatgaattac cgacagttga tttgcttgat tctataccaa ctgtggatca
     8641 aagtgatgag tataaaaaaa ttgaaaaaaa tatcggcgta ttagaacaaa ctttccaaag
     8701 tttcggtgtc gatgccaagg tcgtcaaagc cagcttagga ccatccgtca caaaattcga
     8761 agtccagccg gctgtagggg taaaagtaag taaaattgtg aacttaacag atgatatcgc
     8821 gcttgcttta gcggcgaaag atgtccggat ggaagcaccg attccaggta aatcactgat
     8881 tggtattgaa gtaccaaatg gaaagatcag tatggtttcc tttagagaaa tcatcgaagc
     8941 acaaccaaac cacccagata aattactaga agtaccttta ggacgtgatg tttctggacg
     9001 ggtacaaacg gctgatcttt cgaagatgcc tcacttactc gtggctggtt cgactggtag
     9061 tgggaagtca gtagcaatta atgggattat cacaagtata ttaatgcgtg ctaagcccca
     9121 tgaagtaaaa ctgatgatga tcgatccgaa aatggttgaa ttgaatatgt acaacggcat
     9181 acctcatctg ttgacgccgg tcgtgacgaa ccctagaaaa gcagcgcaag ccttacaaaa
     9241 agtagtacaa gaaatggaag aacgatatga aaaattcgcg gcaaccggag taagaaatat
     9301 ttctggttac aatgaatttg ttcaacagaa aaacttggaa aacggaacga aacatccaac
     9361 cttaccattc atcgtagtga tcgtagatga attagctgat ttgatgatgg tagccagcaa
     9421 tgaagttgaa gatgcgatta ttcgtttagc gcaaatggct cgggcagcgg ggattcatat
     9481 gatcctagct actcaacgtc caagtgtgga tgttatcaca ggaatcatca aagcaaatgt
     9541 accttccaga atggcatttg cggtatcaag cggtacagat tctcggacga tcattgattc
     9601 taatggggca gaaaagctct tagggcgagg agatatgcta ttcttgccaa tgggtgagaa
     9661 taaaccgatc cgtgtgcaag gagcattcat ttctgaccac gaagtagaac gggtcgttca
     9721 atttgttaca gatcagcaag aagctcatta tgaagagaaa atgatgccga cagatgaagt
     9781 tgaaacgaca ggtgctccag aacagccaca agatgagctg tttgaagaag caaaagcact
     9841 tgtcgttgaa atgcaaacgg caagtatttc ccttctccaa agaagatttc ggatcggtta
     9901 caaccgagca gcacgtttag tagatgaact ggaagctcat ggagttgttg gcccgtcaga
     9961 aggaagcaag cctcggaaag tattcattga gcaggaagaa acatcagaaa cgttgccgga
    10021 cgataatgac atgtaaaact tgtgtgatga agggttgaaa aacgaataac caataaaaag
    10081 acaaggcatg atgataccaa aagaaaaaat ggtttttttc catcttttct aatacggtat
    10141 gatgtcttgt ctttttagtt tttgtgagat taaagttcta ttcgatttat ttgtgaagta
    10201 ttgcattaag gaaatgaatc atgtatagta tgtagcgaaa ctttgagaaa aagagggaaa
    10261 atactttgga aaaacagtcc atttatggct tgacgaatga agaattaatc aattggttta
    10321 tagaaaacgg cgagaaaaaa ttccgagctg cccaagtttg ggaatggttg tatcaaaaac
    10381 gtgtaagtaa tttcacagaa atgacgaatc tatcgaaaca attgatcgaa aaattatcgg
    10441 agcattttat catcaatcct ctaaaacaga tggtcgtcca agaagcttcg gatggtacag
    10501 taaaatattt gtttgaactc cctgataaaa acatgattga gactgtactg atgagacaag
    10561 aatatggctt gtcagtttgt gtgacgacac aagtaggatg taatatcggc tgtacgtttt
    10621 gtgcaagcgg gctgctcaaa aagaaccgtg atctgactgc tggagaaatt gttgcacaga
    10681 tcatgatggt ccaacattat tttgatgagc gcaatcttgg cgaacgcgtt tcacatgtcg
    10741 ttgtaatggg gatcggtgag ccatttgata attacgacaa tgtaatggac tttttgcata
    10801 tcatcaatga tgccaaagga ttggcaattg gtgctcggca cattacagtt tccactagtg
    10861 gattagcaca caaaatcaag gaattcgcta ataacggctt gcaagtcaat ctggctattt
    10921 ccttgcacgc gccgaataat gaggtgcgaa catcgatcat gcggatcaac cgaagtttcc
    10981 caattgagaa attgatggaa gctgtggatg agtatttaga gaaaacaaac cgtcgaatca
    11041 cgtttgaata tatcatgttg aatcaagtaa atgatagacc agagcatgcg caacaattag
    11101 cagatctatt aaaagataag aaaaaattag catatgtcaa cttgattcca tataatccag
    11161 tcagtgaaca tgatcaatat tctcgcagtc caaaagctga tgtattgaga ttttatgatg
    11221 tattgaagaa aaacggcgta aactgtgtga tacgtaaaga acatggtaca gatattgatg
    11281 cagcttgcgg acaattgcga agcaagcaaa tgaaaaaaac agttgccaaa taatcgtatc
    11341 ctactcgttt tttcaattac cataaaagga gcagttttct taattaagga actgttcaac
    11401 ttaaataaga gaaaagattc tatagcgaaa gctataaagc tttttttgac aagaaaaacc
    11461 ctcatcaagc aaagaaatgt cctcaatttg atgacagcct gcttgatggg ggttttagca
    11521 attatttaac tgattttctt atatccttgc tggctttctt gtgtagcatg ataagtttct
    11581 acgtcaagaa tggcctgttt aaatttttct cgggtgattt cataaggcac atgtttaata
    11641 tcatttgttg ccatggcttt atctaagatt gcttctaaat cggtcgtgac attgagattc
    11701 atgtcggtta acgatactgg aagttggttc tttttcataa acacatagat tttatcaaac
    11761 aaatcgaact gctgatccat catcaataag gtcagtacac cataactgac agatgctccg
    11821 tgaaggtatc cctccatttt ttctagaaca gtcgtaccgt aatagaatga gtgtgccata
    11881 gcagaattat agttattgtt aactaaaaca gaaaccaatc cggtagtaac aatgatatcc
    11941 gaaacgactt gttcaattgc atatgaggga cgatcgtcgg cactatcttt caatgcttgc
    12001 gtaccataag tcaagagacg atcaatgcac atttttccga tttggattcc taatctatcc
    12061 gtgtagccta gatcatcgcc acgagcagaa aaagtagatt cgacttcttt ggataaagca
    12121 tcgccaatac ctgcccataa gtatttatca ggtgcttcgg caataattgc tgtatcaatg
    12181 aaggtgtgta caggtggttt tgaaggaaag aagagtccgt ccattacgcc ttcttcgtta
    12241 tagatgacac ataccgcagt gacgggcgaa cagttcgaac caatagtggg aaaggtgaag
    12301 actggtttag ctagtttttc accaatgact tttacagtat cgcaagcacg gccaccaccg
    12361 actgcaaaca ggacgtctgc tttttggaca ctttcatgct ggatcaactg ttcaatattt
    12421 ccataagttg aattaccccc gtaccagaga ctggcgacaa cctctatttc ctgtgtcaaa
    12481 gctactttta ttttgttttc tgcctttgaa agtgcagtct tccctccaat cagcactact
    12541 tttgtcccat attctttgac gattccattt gctttttcat acgcatctgt tccaatggta
    12601 taatttggta agaaaacact ttgactcata ttcatttcct tctttcttcg ttgaaacggg
    12661 ttaaaatttt aaagagtgat cgatctgact gcggctgtag atggagtaga ggtgaaccta
    12721 agacaggata agtgatgaat aaggtggcat gaacaccttg aaatcatcaa taagta
//
LOCUS       DAZKLD010000086        12575 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.086, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000086 DAZKLD010000000
VERSION     DAZKLD010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12575)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12575
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.086"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     regulatory      188..354
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00380"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="The M-box (ykoK) riboswitch appears to function as
                     a genetic 'off' switch that is important for maintaining
                     magnesium homeostasis in bacteria; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="magnesium"
                     /db_xref="RFAM:RF00380"
     gene            502..1059
                     /locus_tag="ACG8KR_001910"
                     /pseudo
     CDS             502..1059
                     /locus_tag="ACG8KR_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303250.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
     gene            1117..3156
                     /locus_tag="ACG8KR_001911"
     CDS             1117..3156
                     /locus_tag="ACG8KR_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HFQ0201272.1"
                     /translation="MKRSDKHGKNRTGAYIAGAVILIAAAGGGYFYYQHYQETQAVEA
                     GEKTVEQFVQALNKGDYNKAAEMTSKKAANKSALSEKEILDQYQNIYGAADVKGLQIS
                     NLKVDKKDDSTYSFSYKAKMNTSLGELKDLSYKGTLDRNDGQTTINWQPNLVFPEMEG
                     NDKVSLTTQEAARGNIIDRNGEPLATTGKLKQLGVVPSKLGDGGEKTANIKAIASSFD
                     LTEDAINQAISQSWVQPDYFVPLKIIDGATPELPAGATIQEVDGRYYPLGEAAAQLIG
                     YVGDITAEDIDKNPELSSNGKIGRSGLEMAFDKDLRGTTGRKLSITDADGVEKKVLIE
                     HEVQNGKDIKLTIDAKAQKTAFDSLGGKAGSTVATTPKTGDLLALASSPSYDPNKMTN
                     GISQEDYKAYEENPEHPFISRFATGYAPGSTFKIITAAIGLDNGTIDPNEVLTINGLK
                     WQKDSSWGSFKTTRVSSDVSQVDLKTALIYSDNIYAAQETLKMGEKKFRTGLDKFIFG
                     EDLDLPISMNPAQISNEDSFNSDILLADTGYGQGELLITPIQQAAMYSVFTNNGTLVY
                     PKLIADKETKDKKNVISETAVQTIVPDLREVVQDVNGTAHSLSALGIPLAAKTGTAEI
                     KEKQDVKGQENSFLFAFNLDNQGYMMVSMLENKEDDDSATKRASELLQYLNQNYQ"
     gene            complement(3265..4416)
                     /locus_tag="ACG8KR_001912"
     CDS             complement(3265..4416)
                     /locus_tag="ACG8KR_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HFQ0201273.1"
                     /translation="MEFIGILCLILISTTIGSHISRRLGIPAVIGQLLVGVLLGQAGL
                     NWVKPDILVHDFSEIGVFLLMFLAGLESDLSLLKKYFRPGMFVALLGIIFPVVFGWLT
                     GKAFQVSGNEAIFFGIILAATSVSISVEVLKELNVVNTKEGSTILGASVVDDILVVLV
                     LSFSLSFLTGEASSNASLPLVLIEQLIYFVFIFLLVKWIAPFLLSFAEKVYANSSIII
                     VSLVICLGMSYVADLVGLSSVIGAFFAGIAVSQTKVKEEVYHNVEALGYAVFIPVFFV
                     SVGLEVDFSDFSEQLLFISILTLVAILTKLLGGYFGAQIAGFSQHSSLMVGAGMISRG
                     EMALIILQIGQQSQLIEPQYYSPLVIVILLSTLISPLVLKYFTKKVYEV"
     gene            complement(4570..5100)
                     /locus_tag="ACG8KR_001913"
     CDS             complement(4570..5100)
                     /locus_tag="ACG8KR_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HdeD family acid-resistance protein"
                     /protein_id="HFQ0201274.1"
                     /translation="MFETIKQNIQKYALLRAAVYIISGIAIVLNPSAVFHFIGYLITA
                     YLVLLGLLNLFEAYRFKRKTGSWSIGLFSGLFFLILALIVYAFAPAIVSILPILLGLA
                     VIINSLFQLFFSMNTKAKSWSVYSSILLLIGGFVLLFNPFKSLMLLFQIFGFILIFMG
                     IQEIINFIRIRKMTIR"
     gene            complement(5199..5549)
                     /locus_tag="ACG8KR_001914"
     CDS             complement(5199..5549)
                     /locus_tag="ACG8KR_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HFQ0201275.1"
                     /translation="MAQNRIFTTSFASVYPMYVAKAERKDRIKEEVDEIICWLTGYSQ
                     TELEYQLQKQVTFEQFFAEAPVPNPSRSLITGVVCGVRVEDIEDNLMKEIRYLDKMID
                     ELAKGKKMEKILRS"
     gene            complement(5706..7235)
                     /gene="ahpF"
                     /locus_tag="ACG8KR_001915"
     CDS             complement(5706..7235)
                     /gene="ahpF"
                     /locus_tag="ACG8KR_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008785 - alkyl hydroperoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000302 - response to reactive oxygen
                     species [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HFQ0201276.1"
                     /translation="MLDKETKQNLEQYLALIESPIVFSVSLDNSENSQKLAEFTKEIA
                     EMSPKISWEKRDSMRTPSFSINPVGKESGIVFAGIPLGHEFSSFLLAMLQISGRAPKI
                     SESLSLKIKKITQTLHFDSYVSLTCHNCPDVVQALNILAVLNPNITHTMIEGGMFQKE
                     IDDKKIMAVPTVFLNGEEFSSGRMTIEQIVEKITGPLIEEELFEKEPYDVLVIGGGPA
                     ASSAAIYAARKGIRTGLVADSFGGQVVETLGIENMIGTSYTEGPKLMQQVESHVRSYP
                     IDIMMNQQAVSLNKEQHFINIGLANGISLKAKTAIIATGAHWRSINVPGEKEFKNKGI
                     AYCPHCDGPLFKGKEIAVIGGGNSGVEAAIDLAGLAKHVYVLEFLPELKADQILQDKL
                     VSLANVTVIKHAATKEISGTNQVESLSYIDRRTMEMHTLAVSGVFILVGLLPNTDWLD
                     GTIEMNPRNEIVTEKNGATNIPGVFAAGDCTDSPYKQIIVSMGSGATAALGAFDYLMR
                     T"
     gene            complement(7245..7808)
                     /gene="ahpC"
                     /locus_tag="ACG8KR_001916"
     CDS             complement(7245..7808)
                     /gene="ahpC"
                     /locus_tag="ACG8KR_001916"
                     /EC_number="1.11.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102039 - NADH-dependent peroxiredoxin
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HFQ0201277.1"
                     /translation="MSLIGKKIEEFNAHAYHQGEFLEVSEKDLMGNWSILCFYPADFT
                     FVCPTELEDLQEQYSTLKSLGVEVFSCSTDTHFTHKAWHDTSDAIGKIEYVMIGDPSH
                     QISRIFDVLDEEQGLAQRGTFIIDPDGIVQAMEINADGIGRDASALIDKIRAAQYVRT
                     HPGEVCPAKWKESGETLKPSFDLVGKI"
     gene            complement(7952..8614)
                     /locus_tag="ACG8KR_001917"
     CDS             complement(7952..8614)
                     /locus_tag="ACG8KR_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFQ0201278.1"
                     /translation="MKSSLQSQAYQAIRKQIIYADLEPGRKISEKALEEHLAIGRTPI
                     REALIQLRQQELVYTIPQSGTYVSKINLVSAENARFVREQLEEQIMIECCSKLDTQTR
                     IVLETILEEQEKAVKINDERAFFRADNLFHEACFEIAGRKEVWNWLDDHNTHLERFRW
                     LRVTIQGLPWETIMEQHYDLLKALVDKKPEEARFQTGLHLHMMLKEQETVTQRYPEYF
                     QQ"
     gene            8798..10444
                     /locus_tag="ACG8KR_001918"
     CDS             8798..10444
                     /locus_tag="ACG8KR_001918"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFQ0201279.1"
                     /translation="MIDLNNWRNFIPELSQKEVEIPHFDRASLRENGQKNPVWIHFGG
                     GNLYRGFHAEIAQRLADLGELSTGVIVCETFDEEIIERAYQDFDDLILEVVMPSSGEF
                     VKRILAATADSLYCHPDCSEHFDKMIRHFENPSLQLVTFAITEKGYRLKDTNNKYIGP
                     IAVDIKNGPKKPSHTMSILAVLLYKRFLSGAWPIALVSTDNFSQNGSQLRTSLLTIAK
                     EWQKEGYVQNTFIQYLSNEEIVAFPWTMIDRITPNPSLSVSKMLAKEGFSNMSIFQTA
                     KGNNIAPFVNTEDIHYLIMEDHFPNGRPKFEKAGVFLTDRATVDKADTMKVTTCLNPL
                     HTSLAIMGCLLGYRSIAEEMKDPDLSLLVSEIGYNEGLPVVESPGIIDPKIFLEEVIT
                     VRFSNPNIPNMPQRIATDTSQKVAIRYGETIKKYMEIEVKDPQTLVFIPLTLAAWLRY
                     LIGIDDQGKPFIPSPDPLLTDLQHKLSDIQLGTQDTSHIHEKMAPILSNKEIFGVDLY
                     QAELGRKIEEMFIKMVAGPNAVRKTLQDYLQEHGGNLSWK"
     gene            10435..11517
                     /gene="uxuA"
                     /locus_tag="ACG8KR_001919"
     CDS             10435..11517
                     /gene="uxuA"
                     /locus_tag="ACG8KR_001919"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFQ0201280.1"
                     /translation="MEMTFRWYGSQEEKITLDEIRQIPAVKGIVGTLMDIPVGEVWPR
                     ERIKELKEEIESRGLTLKVIESVNVHDAIKIGLPSREQYIENYKQTIRNLAEYGIEVI
                     CYNFMPVFDWVKSDLDYRLEDGSSTLAFISDDIPDDPREIVARIEHSSNEFGLPGWEP
                     ERLAPIKSLLEAYASVDEEKLRENFAYFLQSIIPTCEEVGVKMAVHPDDPPYSIFGLA
                     RIVKNREDLDWLCRIVDSPYNGITLCTGSIAEDPENDVYGILAEFCQRDRIPFAHVRN
                     IKFLANRDFYEAPHKSEYGSLDMYRILKTMYDNGFNGYIRPDHGRMIWNETGRPGYGL
                     YDRALGAVYLNGLWEAIEKGNKSKDN"
     gene            complement(11625..>12575)
                     /locus_tag="ACG8KR_001920"
     CDS             complement(11625..>12575)
                     /locus_tag="ACG8KR_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HFQ0201281.1"
                     /translation="FKKEVLDRLNSGMIDKLYFLLKICKENFPATSVKDVQKEFTEYE
                     YIDFFIDLSVDILRPNLPICREQRILVNILARLSITKIESEETSLEQYKLLKKANVPH
                     VLYSREMVESLCTVFDLFIPEKEKILYSLAFARNKLYNGFLNFNQPNTPLPTFMLYQE
                     HSVLKEVQTAIEQFYMDFREQNQHLLPVILEKENVQWMVEDLVHLYDRYKKQPRIVIG
                     VNYTRDFYISKDLIVKIEQIFSNESIKIQNDFMENCNIVISDCPLKQLPSHIKKIYIL
                     KGIITPEDWKKVIMRISEYIFELKEEALMDQSKKENQVTS"
BASE COUNT         4202 a         2318 c         2346 g         3709 t
ORIGIN      
        1 cacataaaac taaaaaatta tcaaagaatc ctggtgcaac tgaaagaatt tgcaacggga
       61 ttctttcaac ttatttgtta tttttttcta aaaaatatta aagattgtta aatttcacta
      121 tttcatttca aattccattg cctaatcatt aggaaaagtc tatcatgaac tcgtaaatag
      181 aatatagtaa taaccttgtt aggtgaggct cctatacaaa cataggctgt tgcccagaaa
      241 cgtcgaaagt cgccaatggg taaaacagaa actgttggat taaggctttt tccaatagct
      301 aagaatgttt tctttacgtt gtatagtgct aaaactcgga cgatgagatg atgctaggtc
      361 agaattcttg ttcgaaaagg tttctgacct tttctttata tttttgtgac tgacttattt
      421 gatgagacaa actcgctcct cacctgacaa gtggattgct gtgtgaggag gagatgcggt
      481 gaaaaaagat cgattggtgg aggtcgctcg gattcacttc tggttgcaca atacaaccag
      541 aaaacacaac aagcaaaact gatctctatc atgagagatt catatgtcga tattccaggt
      601 tacggaatga ataagataaa tgcagcttac tcttatgggg gagttgattt attaaatcaa
      661 acattaaagg aaaatttcaa atttgaagct ccctattatg caagtatcac atttcaagat
      721 tttatcgatt gcgttaatga actgtttcct ggtggagtca agatcgatgc agaaaaatct
      781 ctagatttag atggcgtata tataaacaaa ggagagcaag tgatggatgg taatacttta
      841 ctccagtatg ctcgatttcg ggaagacaaa gaaggagact tcgggaggat cagaaggcag
      901 cagcaagtga tcaaagctat ctctcagcag ctgaaagacg taacttcaat attcaagcta
      961 cccaaagcgg ttgggaaatt acttggtagt atccaaacga accttccaga aagtgtgctt
     1021 ctggattgtg gtatggattt cctcaaagac atatgttaga ataaacaggt ataaatagtg
     1081 aaataaagga atgacaagca agagaaggag gaaaaaatga aaagaagtga caagcacggc
     1141 aaaaatcgaa caggcgctta tattgccggc gcagtgatct taatagcagc tgcgggtggc
     1201 ggttattttt actaccagca ctatcaagaa acccaagcag tagaagctgg agaaaagaca
     1261 gttgagcaat ttgtccaagc tttgaacaag ggagattata acaaagctgc agaaatgaca
     1321 tcaaaaaagg cggcaaataa aagtgcatta tctgaaaaag agatcttaga tcaataccag
     1381 aatatatacg gtgctgctga tgtcaaagga cttcagatat cgaatctaaa agtagataaa
     1441 aaagacgatt ctacttacag tttttcatat aaagcaaaga tgaatacctc attaggtgaa
     1501 ttgaaagatc tttcttataa aggaacatta gacagaaatg atggtcaaac cacgatcaac
     1561 tggcaaccca acttggtttt tccagaaatg gaaggaaatg acaaagtaag cctgaccaca
     1621 caagaagcag caagagggaa tattatagat cgaaatggtg aaccattagc aacaacaggc
     1681 aaactaaaac aattaggagt cgttccaagt aaacttggag atggaggcga aaaaacagcc
     1741 aatatcaaag cgattgcttc ctccttcgac ttaacagaag atgctatcaa tcaggcgatt
     1801 tcacaaagct gggtacaacc cgattacttt gtcccattga aaatcattga tggagcaacg
     1861 ccagaacttc cagctggagc taccattcaa gaagtagacg gcagatatta tcctttgggt
     1921 gaagcagctg ctcagctgat tggttacgtg ggagatatca cagcagaaga tattgataag
     1981 aatccagaat taagcagtaa tggaaaaatc ggtcgctcag gtttggagat ggcttttgat
     2041 aaggatcttc gtgggacaac aggtaggaaa ttaagcatta cagatgcaga cggtgtagag
     2101 aaaaaagttc tgatcgaaca tgaagttcaa aatggcaaag atatcaaatt gacaatcgat
     2161 gcgaaggcac aaaaaacagc ttttgacagt ctaggaggaa aagctggatc aactgttgcg
     2221 acaacgccaa aaaccggtga tcttcttgcg cttgctagct ctccaagcta tgatccaaac
     2281 aaaatgacaa acgggatctc acaagaagat tacaaagctt atgaagaaaa tcctgaacat
     2341 ccattcatca gccgatttgc gacaggttat gctcctggat ctacgtttaa aataattaca
     2401 gcagcaatcg gtctcgacaa cggcactatc gatccaaatg aagtgttgac gatcaacggg
     2461 cttaaatggc aaaaagacag ttcttgggga tcttttaaaa caacgcgtgt cagtagtgat
     2521 gtatcacaag tagacttaaa aactgctttg atctattccg ataatatata tgcggcacaa
     2581 gaaacgttga aaatgggtga gaaaaaattt cgtacaggct tagataaatt catttttggt
     2641 gaagaccttg atttgccaat tagtatgaat ccagcacaaa tttctaatga agatagcttt
     2701 aactcagata tcttgctagc tgatactgga tatggacagg gcgaacttct aattactcct
     2761 atccagcaag cagcaatgta ttctgttttt accaacaatg gcacacttgt ctatcctaaa
     2821 ttgattgcag ataaagagac aaaagataag aagaatgtca tcagcgaaac agcagtacaa
     2881 acgatcgtgc cagatctgag agaagttgtg caagatgtaa atggtacagc acattctctt
     2941 tctgctttag ggattccgtt ggcagcgaaa actggtacag cggaaatcaa agaaaaacag
     3001 gatgtaaagg ggcaagagaa cagtttcttg tttgctttca atcttgataa ccaaggttat
     3061 atgatggtca gcatgttgga aaataaagaa gatgatgatt cagcaaccaa acgagcatcg
     3121 gaactattgc aatacctcaa ccaaaattat caataaaaaa ttgcttttta agtacagtaa
     3181 aaggactgtg gcgttatcgt cacagtcctt ttttcaaata aacggttttc acgatgtctc
     3241 tttcacaact tctttttgat ttactcacac ttcataaact ttttttgtaa aatatttgag
     3301 aacaagtgga gaaataagtg tacttaataa aatcacgata actaaaggtg aatagtactg
     3361 tggttcgatc aattgacttt gctgtccaat ttgtaaaatg atcaaagcca tttctccacg
     3421 cgaaatcata ccagcaccga ccatcaatga actatgttga gaaaaaccgg caatctgagc
     3481 accaaaataa cctccaagca atttggtcaa aatcgctaca agcgtcaata tagaaataaa
     3541 gagtagttgt tcactaaagt cagaaaaatc aacttctaat cctacactga caaaaaatac
     3601 tggtataaat actgcatatc ctaaagcctc tacattgtgg tatacttctt ctttaacctt
     3661 tgtctgactg acagctatcc cagcaaaaaa agcaccaatg acagagctaa gcccaacaag
     3721 atccgctacg taagacattc ctaaacaaat aactagcgat acgatgataa tagaagaatt
     3781 tgcgtacact ttttctgcaa atgaaagtag gaaaggcgca atccatttga ctaataagaa
     3841 aataaaaaca aaataaatta gctgttcaat caaaacaagc ggtaaagaag cattgctaga
     3901 agcttccccc gttagaaaag ataggctgaa acttagaact aaaacgacta aaatgtcatc
     3961 taccacagaa gcacccaaaa ttgttgatcc ttcttttgta ttcactacgt ttagttcttt
     4021 taatacttct actgaaatac tgacagaagt tgccgcaaga ataatcccaa agaaaatcgc
     4081 ttcatttccc gaaacttgga atgctttgcc tgtcaaccag ccaaaaacaa ccgggaagat
     4141 gatcccaagc aaagcaacga acataccagg tcggaaatat tttttcaaaa gagataaatc
     4201 actttccaga ccagctaaaa acattaataa gaacacacca atctccgaaa agtcatggac
     4261 tagaatatca ggtttgaccc aattcaatcc agcttggcct agcaaaacac caacaagtaa
     4321 ttgtccaatc actgctggga tacctaatct ccgcgagata tgtgagccaa tcgttgttga
     4381 aatcaatatc aggcataaaa taccaataaa ttccattctc atcattcctt tgtataaaat
     4441 atagcccgaa cttagcataa catacattat tgaaaaaata ggaaaatata ctcacaaaaa
     4501 aaccaacaat ccaaaagatt tttttggatt gttggttttt atacggtcta ttttaataac
     4561 taaaaatgat taacgtatag tcatttttct tattctgatg aaattgataa tctcctggat
     4621 gcccatgaaa atcaaaataa agccgaatat ttggaataga agcatcaaac ttttgaatgg
     4681 attgaacaat aagacgaatc cgccaatcaa caacagaata ctactataaa cactccaact
     4741 tttagctttt gtattcatgc tgaagaacaa ctggaacaag ctgttgatga taacagctaa
     4801 tccaagaaga atcggcagga tagaaacgat tgcaggtgca aaagcataga cgatcagagc
     4861 taagatcagg aaaaaaagac cactgaacag accgatactc caagaacctg ttttcctttt
     4921 aaaacggtaa gcctcaaaaa gattcaacaa cccaagcaaa acaagatagg cggtgatcag
     4981 ataaccgata aaatgaaaaa cagcagaagg gtttagcaca atcgcaatcc cagaaataat
     5041 ataaacagca gctcttaata aggcgtattt ttgaatattt tgtttaatcg tctcaaacat
     5101 ataagaaaac ctcctttgac ttattttaac gtgaactagt cattttacca aattaaatgt
     5161 tacaaaatga gcgaaggaga agaatatctc tggaaaaatc aacttctgag gatcttttcc
     5221 atttttttgc cttttgctag ctcatcgatc attttatcta aataacgaat ttctttcatc
     5281 aagttatcct cgatgtcttc tactcgtaca ccacaaacaa cacctgtaat caaggaacgt
     5341 gaaggattgg ggacaggggc ttccgcaaaa aattgttcga aagtcacttg tttttgtagt
     5401 tgatattcta gttctgtttg ggaataaccc gtcagccaac aaatgatttc atctacttcc
     5461 tcttttatac gatcttttct ctccgctttt gctacataca taggataaac gcttgcaaaa
     5521 cttgtcgtaa aaatacgatt ttgcgccata atcagtcacc tttctttttt tcaaattatt
     5581 tactacttat agtatataag aaaaagagaa gaaaaagcga ttattcaaaa aaacaaaaag
     5641 gattgtgaca aaattcttgt caccatcctt ttttccgctc tctgactttt tacaatcttc
     5701 atagctcaag tgcgcatcag ataatcgaat gctcctaaag ctgcagtagc tcctgaaccc
     5761 attgaaacaa tgatttgttt gtatggacta tctgtacaat caccagctgc gaaaacgccg
     5821 gggatattcg ttgcgccatt tttctcggtc actatttcat ttcttggatt catttcgatc
     5881 gttccatcta gccaatccgt attagggagt aagccgacta gaatgaatac tcctgaaaca
     5941 gctagtgtat gcatctccat cgtacggcga tcgatatagg agagagattc tacctgattc
     6001 gtaccgctga tttctttcgt cgctgcatgt ttgataacag tgacattcgc aagagagact
     6061 agtttatcct gtaagatttg atctgctttt aattctggta agaattctag aacatataca
     6121 tgttttgcta acccagccaa atcgattgct gcttcaacac cagaattacc accaccgata
     6181 acagcaatct cttttccttt aaataaaggg ccgtcacagt gtgggcaata agcaattcct
     6241 ttatttttaa actctttttc gcctggtaca ttgatagaac gccagtgcgc acctgtcgcg
     6301 atgatggcgg tttttgcttt aagtgatata ccgttagcta agccaatatt aataaaatgt
     6361 tgctctttat ttaaggaaac ggcctgttga ttcatcataa tgtcgatagg gtaactacgc
     6421 acatgacttt ctacttgctg catcaatttt ggaccttctg tgtaagaggt accaatcata
     6481 ttttcaatcc ctagtgtttc aacgacttgt ccgccgaaag aatcagcgac aagtcctgta
     6541 cggattcctt tacgtgctgc atagatagct gcactactag ctgctggtcc accgccaatc
     6601 actaatacat cataaggttc tttttcaaac aactcttctt caatcaaagg gccagtgatt
     6661 ttttcaacaa tttgttcgat cgtcatgcgt ccgctactaa attcttctcc atttaaaaac
     6721 acagttggca ctgccatgat ttttttatca tcgatttctt tttggaacat cccaccttca
     6781 atcatcgtat gagtaatatt tggatttaat acagctaata tattcaatgc ctgtacaacg
     6841 tcggggcaat tatgacaagt caagctaaca taactgtcaa aatgcaatgt ttgtgttatt
     6901 tttttgattt taagactcaa agattcactg attttcggtg cccgtccact aatttgtaac
     6961 atagccagta aaaaagagga aaattcatga ccgaggggga taccagcaaa aacgattcca
     7021 ctttctttgc caactggatt gatagaaaaa ctaggtgtgc gcatcgaatc tcttttttcc
     7081 caagagattt taggagacat ttctgctatt tccttcgtaa attcagctag tttttgacta
     7141 ttttcactat tatccaaact aacagaaaaa acaataggag attcaatcag tgctaaatat
     7201 tgttcgagat tttgtttagt ttctttgtct aacatatcca ccacctaaat ctttccaaca
     7261 agatcaaaac ttggtttcaa tgtttcgcca ctttctttcc attttgccgg acaaacttca
     7321 cctgggtggg tacgtacata ttgagcagcg cgaatcttat caatcagtgc actagcgtca
     7381 cgaccgattc catctgcatt gatttccata gcttgaacga ttccgtctgg atcaataata
     7441 aatgttcctc gctgagctaa tccttgctct tcatccaaaa catcaaaaat acgagaaatc
     7501 tgatgagacg gatcaccgat catcacgtat tcgattttac cgatagcatc tgatgtatca
     7561 tgccatgctt tgtgagtaaa gtgggtatcg gtagagcaag aaaagacttc aacacctaaa
     7621 gatttcaatg tactgtattg ttcttgtaag tcttctaatt cagtaggaca aacaaaagta
     7681 aaatcagctg gatagaagca aagaatactc cagttcccca taagatcttt ttcactgact
     7741 tctaaaaatt ctccttgatg ataagcatgt gcattaaatt cttcgatttt tttcccaatc
     7801 aatgacatag taaaacctcc aataataatt atttatttat aattattgta atttagttgg
     7861 agttgcaaag tcaatggata ttttgttatt taataaagat tactcaaaaa cgaaggttaa
     7921 ttataaaaat agaagaggtc gcaagaaaat gctactgctg gaaatactcg ggataacgtt
     7981 gagtaacagt ttcctgttct ttcagcatca tgtgtaagtg caatcccgtt tgaaatcgag
     8041 cttcttctgg ttttttatca actaaagcct tgagtaaatc ataatgttgt tccatgatcg
     8101 tttcccatgg aagcccctga atggtcactc gtaaccagcg gaaacgttct aaatgggtat
     8161 tatggtcatc taaccagttc caaacttctt ttcttcctgc aatttcgaaa caagcttcat
     8221 gaaataaatt atctgcacgg aaaaaagcgc gttcgtcatt tattttgact gctttttctt
     8281 gttcttctaa aatcgtttct agcactattc gtgtttgtgt gtccaatttg gaacaacatt
     8341 caatcatgat ttgctcttcc aactgttctc ggacaaagcg agcattttct gcagaaacaa
     8401 gattaatttt ggaaacatac gtaccacttt gaggtatcgt atacacgagt tcttgctgac
     8461 gtagttgaat caatgcttcg cgaatgggag tccggccgat agcaagatgt tcctccagtg
     8521 ccttttcaga tattttcctt ccaggttcta agtcagcata gataatttgt ttacgaatcg
     8581 cttgataagc ttgtgattgc aagctgcttt tcatctttat tcctccaaaa tcatttctat
     8641 gaacgtagta taacagaaga ggtctgaaaa acaatctggt atgtcacatt aactagtaaa
     8701 tttgtataaa tagtaaaatt ttggttgaca tagcgatttt tattgcataa aattcacttg
     8761 aatacaaaaa ctaatatgtt agtttaggag gggaaaaatg attgatttaa acaactggag
     8821 gaactttatc cctgaattat ctcaaaaaga agtagaaata cctcattttg accgtgcatc
     8881 attaagagaa aatggacaaa aaaatcctgt ctggatccat ttcggtggcg gaaatcttta
     8941 tcgaggattt catgcagaaa tcgctcaacg tttagcggat ctaggcgaat tgtccactgg
     9001 tgtcattgtt tgtgaaacat tcgatgaaga aataattgaa agggcttacc aagattttga
     9061 tgatttgatt cttgaagtgg tcatgccaag cagtggagag ttcgtcaaac gtatattagc
     9121 tgcgacagct gacagtctgt attgtcatcc agattgttct gaacactttg ataagatgat
     9181 tcgacacttt gaaaatccta gtttacaatt agttactttt gctattactg aaaaaggtta
     9241 tcgattgaaa gatacaaata ataaatatat aggaccgatt gcagtcgata taaaaaatgg
     9301 tccaaagaag ccatcacata caatgagtat tcttgctgtg ctactttata aacgtttttt
     9361 atctggagct tggccgattg cacttgtctc tacagataat ttttcacaaa acggtagtca
     9421 attacgtact tctttgctaa cgatcgcaaa agaatggcaa aaggaaggtt atgtacaaaa
     9481 tacatttatc caatacttat cgaatgaaga aattgttgcg tttccatgga cgatgattga
     9541 tcgtatcacc ccgaatcctt ccttgtcagt tagcaaaatg ttggcaaaag agggcttttc
     9601 aaatatgtcg attttccaaa ccgcaaaagg aaataatatt gctccatttg tcaatacaga
     9661 ggacatccat tatttaatca tggaagatca ttttcccaat ggacgtccga aattcgaaaa
     9721 agctggggta ttcttgactg atcgtgcaac ggtagataaa gcagatacga tgaaggtgac
     9781 gacttgcctt aatcctttgc atacgtcttt agctattatg ggttgtttac tggggtatag
     9841 gagcatagct gaggagatga aagatccaga tttatcgctg ctagtttctg aaatcgggta
     9901 caatgaaggc ctcccagttg tggagtctcc aggtatcatt gatccaaaga tctttctgga
     9961 agaagtaatt actgtgcgct tttctaatcc aaatattcct aatatgcctc agcgaatcgc
    10021 cacggatacc tctcaaaagg tggcgattcg ttatggtgag acgattaaaa aatatatgga
    10081 aatagaagtt aaagacccgc agacacttgt attcatacca ttgactctcg ctgcttggct
    10141 tcgatacctt attggcattg atgatcaagg aaaaccattc atcccgagtc cagacccact
    10201 attgacagac cttcagcata agctttccga tattcaactg ggtacgcaag atacatcaca
    10261 tattcatgag aaaatggctc ctattttgtc aaataaagaa atttttggtg tagatcttta
    10321 ccaagcagag cttggcagaa aaatagaaga aatgttcatc aagatggtag caggaccaaa
    10381 tgccgtacga aaaacattac aggattactt acaagaacat ggaggaaatt tatcatggaa
    10441 atgactttta gatggtacgg atcacaggaa gaaaagatta ccttagatga aatcagacag
    10501 attcctgcgg tcaaagggat tgtaggcaca ttgatggata taccagtagg agaggtatgg
    10561 cctagggaga ggatcaaaga actaaaagaa gaaattgagt ctcgtgggtt aacactaaaa
    10621 gtaattgaaa gcgtcaatgt gcatgatgcc attaaaattg gattaccttc ccgtgagcag
    10681 tatatcgaaa actataagca gacaatccga aacttggcag aatatggaat agaagtcatc
    10741 tgctataatt ttatgcctgt atttgactgg gtgaaatctg accttgacta ccgcttagaa
    10801 gacggctcat ccacgttagc ttttatatct gatgatattc cagatgatcc aagagaaatc
    10861 gtcgcgcgca tcgaacattc cagcaatgaa tttggattgc cgggatggga acctgagcgc
    10921 ttagctccta tcaaatcctt gcttgaagcc tatgcatcag tagacgaaga aaaacttcga
    10981 gaaaattttg cctattttct ccaatcaatc atcccaacat gtgaagaggt aggggtcaaa
    11041 atggctgtac atccagatga tccgccatac tcgatttttg gattagctcg aatcgtaaag
    11101 aatcgagaag atttagattg gttgtgtcgc atagtagaca gcccttataa cggaatcact
    11161 ctttgtacag ggagtattgc agaagatcca gaaaatgatg tatatgggat tttagcagaa
    11221 ttttgccaaa gagatcgaat cccatttgca catgttcgaa atattaaatt tttagctaat
    11281 cgagatttct atgaggcacc acataaatcc gaatacggtt cattagacat gtaccgcata
    11341 ctaaaaacaa tgtatgacaa cggatttaac ggatacatcc gtcctgatca tggaagaatg
    11401 atctggaatg agacaggaag accaggatac ggcttatatg atcgagcatt aggtgctgtt
    11461 tatctaaatg gattatggga agcaatcgaa aaaggaaaca aaagtaaaga taattagtga
    11521 aaaaaactgt aattttatta tgtaaataag aaggatgttc aaattcttga tacataaatc
    11581 cggaggttgt taataagcat tcggatttat gtatcttatt tggtctagga agtaacttga
    11641 ttttcttttt tcgattgatc catgagtgct tcttctttta attcaaagat atattctgag
    11701 atcctcataa tcactttttt ccagtcttct ggtgtgatga ttccttttaa aatatagatt
    11761 tttttgatat gagaaggcaa ttgtttcaaa gggcagtcgc tgataacgat gttgcagttc
    11821 tccataaaat cattttgtat tttgatcgat tcatttgaaa aaatttgttc tatcttaacg
    11881 atcagatctt tactgatata aaaatcacgc gtatagttca caccaatcac aattcgcggc
    11941 tgttttttgt agcgatcata caaatgtact aggtcttcaa ccatccattg cacattttcc
    12001 ttttctaaaa taactggtaa caagtgttga ttttgttctc tgaaatccat ataaaattgt
    12061 tcaatggcgg tttggacttc tttcaatacg ctatgttctt gataaagcat gaaagtagga
    12121 aggggcgtat ttggttgatt gaagttaaga aatccattat aaagtttatt tcgtgcgaat
    12181 gccaatgagt aaagaatctt ttctttttca ggtataaaca aatcaaatac agtacaaagg
    12241 gactccacca tttctcttga ataaagaaca tgaggaacgt ttgctttttt taaaagtttg
    12301 tattgctcca agcttgtttc ttccgattcg attttcgtga tagaaagtcg agctaaaata
    12361 ttcactaaga tgcgttgttc gcgacaaatt ggcagattag gtcgcaaaat atctacagag
    12421 agatcaataa aaaagtcaat atattcatac tctgtgaatt ctttttgtac gtctttcaca
    12481 gaagttgcag gaaaattctc tttacatatt tttaatagaa aatataattt atctatcata
    12541 ccgctattta gtcggtcaag tacttctttt ttaaa
//
LOCUS       DAZKLD010000087        12483 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.087, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000087 DAZKLD010000000
VERSION     DAZKLD010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12483
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.087"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            147..719
                     /locus_tag="ACG8KR_001921"
     CDS             147..719
                     /locus_tag="ACG8KR_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HFQ0201282.1"
                     /translation="MTENKATQERTPQERLQEEQEHKEHVHHTKINAAAIADHLLGNM
                     HTLHVKLHQYHWYVKGANFFTLHEKFEELYNENEKWFDKLAERLITSGHKPASTTVEF
                     EKYSMLSEDAVNKYAKAEDMVENIIEDFRSTRDLTIRAIRLAQDEGDDALEDTLIAFK
                     NDLDKNIWMLQAFIGKEALEDDDALDEDED"
     gene            864..1568
                     /locus_tag="ACG8KR_001922"
     CDS             864..1568
                     /locus_tag="ACG8KR_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011476586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFQ0201283.1"
                     /translation="MTKHTHHEIGDHAACIRLVPIFNHLEDSAMNYIAEKAHTKQYQK
                     GEFLFRSQEKEDTLYIINRGKIRIYRLADSGKEQLVRILNPGDFTGEWTLFNPDAVHE
                     EYAEATRDTVVCMIQQKDVQDFLEQYPAISLKLLAEMSKRLESSERQTTQVAVESVIT
                     RLVLFLAENVEPEMGNSPTITLPMAKKDIASYLGTTPETISRKFGELEDEGLIQQLSG
                     KRIKIQDLDDLLLYSE"
     gene            1796..2134
                     /locus_tag="ACG8KR_001923"
     CDS             1796..2134
                     /locus_tag="ACG8KR_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0201284.1"
                     /translation="MHCFWVIVAITLSAAGAFFVVPFTKNTIGGEAKTSNVVEADRLA
                     MEKEEEVVKGAGVIGHGKVAYYEASDQGDKSVFTVWFPWSLAGILFSITMTRMTRKIE
                     EMEQIEWDSY"
     gene            2119..2973
                     /gene="lgt"
                     /locus_tag="ACG8KR_001924"
     CDS             2119..2973
                     /gene="lgt"
                     /locus_tag="ACG8KR_001924"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HFQ0201285.1"
                     /translation="MGFVLGVLNPHVVEIGSVVIHWYGVIIAAGILAALQLSTKEAKK
                     KGLEEDTIIDMALWAIPIGLLGARLYYVLFELGYYLQNPGQILAIWNGGIAIYGGLIA
                     GGGTLYWYAKKKGTSLALVLDILAPNVLLAQSIGRWGNFMNQEAHGGPVTRQFLENLY
                     LPEFIIEQMNMNGTYYHPTFLYESLWSLLGFVLIVTIRNRKQLLRQGEVALSYVLWYS
                     VGRFFIEGMRTDSLWIGEWIRVSQALSLALFIGAIVVWFIRRQDYPPISYYSDGNKGQ
                     KKTIKMVN"
     gene            3120..3353
                     /locus_tag="ACG8KR_001925"
     CDS             3120..3353
                     /locus_tag="ACG8KR_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HFQ0201286.1"
                     /translation="MEKAILQLETLSCPSCMQKIEGAVKSVNGIDKDSIKVLFNSSKV
                     KTNFDSAVTSIEEIQKAIENVGYPVLKAKVKAV"
     gene            3551..5455
                     /locus_tag="ACG8KR_001926"
     CDS             3551..5455
                     /locus_tag="ACG8KR_001926"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFQ0201287.1"
                     /translation="MQQYILSKKNVITVISGLLIVLGFFSHFVLENVGLSEWSLIIAS
                     VFGITPIAIQAFQAMKVKVISIDVLVSIAAIGALFIQNYEESAIVTFLFLFGHYLEQR
                     TLNQTRSAIKELTEMAPESALKQMDNGEFEEVEVDDIDEGDILLVKTGAKVPVDGIVL
                     TGEGHINEASITGEAIPVNKLADAEVFAGTILENGTIQIRADRVGEDTTFGKIIELVE
                     EAQDSKSEAERFIDRFSKYYTPAVLVLAIVVWAFSQNIELAITILVLGCPGALVIGVP
                     VSNVAGIGNGARNGVLLKGSEVIQDFSNVDTIVFDKTGTLTVGNPTVAATELYEKDPA
                     ETLGYLASVERESDHPLAKAVLNQIGETNFYPVEGTEVVKGGGIVSSVAGHRVAVGNV
                     ALMEKENVTLSKKVQKDVKRFEQQGNSLVLTAVDGELKVLMGIRDQVRPGVKANLQEL
                     KDLGVKNLVVLSGDNQGTVDVVAGELGLTEAHGYMLPEDKSAYIGKLQQRGQIVAFVG
                     DGVNDSPSLALADIGIAMGSGTDVAIETSDVVLMNSNFSNLPHALGLVKATANNMKQN
                     IVISIGVVLVLLTSVFFSEWMNMSIGMLVHEGSILVVIFNGMRLMKYKLKGRKEQKEV
                     SAPAEKVKIS"
     gene            5765..6127
                     /locus_tag="ACG8KR_001927"
     CDS             5765..6127
                     /locus_tag="ACG8KR_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR/SmtB family transcription factor"
                     /protein_id="HFQ0201288.1"
                     /translation="MEKVCQVTIVHKEKVNVIKKKLDQKDFSNLLVLGKCFSDSSRIK
                     IFYALETYKEMCVCDLAEILTASVATTSHHLRFLKKHGMAKSRQDGKVVYYSLANEDI
                     LSAIRVFLKLSENLSMKS"
     gene            6144..6755
                     /locus_tag="ACG8KR_001928"
     CDS             6144..6755
                     /locus_tag="ACG8KR_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CadD family cadmium resistance transporter"
                     /protein_id="HFQ0201289.1"
                     /translation="MLQSIFSAIAVYISTSIDYLFILLIIFSQSHTKKGIRHIFWGQY
                     LGTGILVAVSLFAAYVLNFIPQDWIIGLLGLIPIFLGIRVAFVGEEEEEEEEVVEKLE
                     SRGTNRLFWTVALITIASGGDNLGIYIPYFTSLAFSEIIIALIVFAISVAVLCYISYK
                     LAKISFVSETLEKYERIIVPIMFIALGIFIMIENGTIQTLLGF"
     gene            complement(6857..7765)
                     /locus_tag="ACG8KR_001929"
     CDS             complement(6857..7765)
                     /locus_tag="ACG8KR_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFQ0201290.1"
                     /translation="MSKSDKDHNHSNQNQPKKNIKIAFFINLIFSISEFFFGFLFNSV
                     SIMSDAVHDLGDSISIGFAWFFQGYSEKKQDERFTFGYNRFSLLGALITGVVLISGSF
                     FMIYRSVPRLIDPQPVNYSGMFWLSLVAIALNGYAAWILSKGTSKNESMLNLHMLEDV
                     LGWIGVLIVSILMNFTEAYRLDPILSILIALYILYKTVPEFFTTMKILLNGSPENVNV
                     KSLADSIKNVPAVKDLSHFHIWSLDGEENALIITVLIDPSDIDKAREIKEEIAEVAHK
                     SSVENHITIEITTSKEELEKGYSHNT"
     gene            complement(7807..8121)
                     /locus_tag="ACG8KR_001930"
     CDS             complement(7807..8121)
                     /locus_tag="ACG8KR_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HFQ0201291.1"
                     /translation="MDIKTTSPIDKGSILSISKLFKVISDPTRLSVLFLLQKKECSVG
                     NIAISLDMEQSAISHQLKTLKDARLVKSRREGKSMLYSLDDLHVFSILEQVLTHVNEL
                     EK"
     gene            8420..10351
                     /locus_tag="ACG8KR_001931"
     CDS             8420..10351
                     /locus_tag="ACG8KR_001931"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFQ0201292.1"
                     /translation="METIQEQQSQEKHNHNHNHDHDHDHGKMPVVLYFVGLALAVIAL
                     FLNEDYQLVQNVLFSIATISAGYHVILLEGLGETIENSKAQKKFIPNSHILMGLAAIG
                     ASLMGNFWEGTLLILIFSGAHFLEDYAEGRSKREITKLLEMNPTTARLILPDGSTKNV
                     DVSELKVGDQLQVLNGDQVPIDGVILSGSTSIDESSITGESIPKEKSKGDGVFGSTIN
                     GTGTFTMKVTKENKDTVFSKILQLVNQNQDNQTKAASIIQKFEPKYVTVVLIAIPLFM
                     LLAPFLLDWTWSQSVYRGLVLLVAASPCALAAATVSATLSTTSNLAKKGVLSKGSSYL
                     SQLADIKAIAFDKTGTLTKGKPEVTNYYFADSVNEENMIDIVVALEKESNHPLADAIL
                     RKFEQKNKLTIEVENQIGKGLTGDYKGRNYRIGKPTSFDDVSDEYSRLNNEWASEGKT
                     VVYVAVDEEVIGLIALMDVPSEYAKETIEYFRKQGIHTTLITGDSEMTGKAVAKQLGI
                     DEVIANVMPEDKSRIIDEQKEKYGVTAMVGDGVNDAPALVNADVGIAMGDGTDVAVEV
                     SDLVLMQNNLSKLVQSHNISSKMNRVIWQNIIFSMAVVAFLIVVSFLGLTDIAISVII
                     HEGSTLVVILNGLRLLKAV"
     gene            10644..11195
                     /locus_tag="ACG8KR_001932"
     CDS             10644..11195
                     /locus_tag="ACG8KR_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFQ0201293.1"
                     /translation="MGYNVQPLRSQQEINDFLFCLRRNKNAERDVFLFLIGINSGLRM
                     SDIVKLKKKDVITSKNPRIVEQKTGKTRILYLSSLQDLIHGYTAPLDPEAYLFPSTKG
                     GHLEVNTVYQMFQKVAKLLGRDDIGTHTLRKTFGYHYYKKTKDVATLMEIFGHSSEKI
                     TKRYIGINEDEISETLLNFRLGF"
     gene            11508..12050
                     /locus_tag="ACG8KR_001933"
     CDS             11508..12050
                     /locus_tag="ACG8KR_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBECR4 domain-containing protein"
                     /protein_id="HFQ0201294.1"
                     /translation="MSIKETTQITRQLNAIYKAAHLLQEHFVDKKVSFVGEVSTVAII
                     FSTTNFMHLCGIDYRRGTHLFFQDALDRKINLQDIQIKTDGTTFQKLQVIGSLDLLLG
                     KHISIVGRGVYSSLRYDAAIRTRKKILALSLKQNGLIYIPISLLNLSSKEIGPGQKVT
                     GIFSEDLTSGELKMIMEVID"
BASE COUNT         4042 a         2093 c         2608 g         3740 t
ORIGIN      
        1 gaagcctggc gtctattaag aaataatttt cttgatgagc gtcaattttt ttaacggaaa
       61 tctatcctat actaaattca gtaaagaaaa aggaggaatc aaagaaagct attttaaaca
      121 aaccaaaatt ataggaggaa taaatcatga ctgaaaataa agctactcaa gaaagaacac
      181 cccaagaaag attgcaagaa gaacaagaac acaaggaaca tgttcatcat acgaaaatta
      241 atgcagcggc cattgcggat catcttttag gcaacatgca tacattacat gtgaaactgc
      301 accaatatca ctggtatgta aaaggagcca atttctttac gttgcatgaa aaatttgaag
      361 agttgtataa cgaaaatgaa aaatggttcg acaaacttgc ggagcgtcta attacttctg
      421 ggcataaacc tgcttcgaca acggttgaat ttgaaaaata ttcgatgctt tcggaagatg
      481 cagttaataa atacgctaaa gcagaagaca tggttgaaaa tattatcgag gacttcagaa
      541 gcactcgtga cctaactatt cgcgcgattc gtttagctca agacgagggt gacgatgctt
      601 tagaagatac attgattgct tttaaaaatg acttagacaa gaacatttgg atgctgcaag
      661 cgttcattgg taaagaagca ttagaagatg atgacgctct tgatgaggat gaagattaag
      721 gtaaaagtag aagctgattt agggattgga attctttcca gtcctttttc cattttcttg
      781 atgtgcatca atttttttgt agtaagagac ttatataata aaagcagcga atataaacaa
      841 aataaataaa gggggagaat gaattgacta aacataccca tcatgaaatt ggcgaccacg
      901 cagcctgtat ccgcttggtt ccaatattca atcacttaga ggatagcgcg atgaattaca
      961 ttgctgaaaa agcccacacg aaacagtatc aaaaaggaga gtttctgttt cgttcacaag
     1021 aaaaagagga tactttatat attatcaatc gtgggaaaat ccgtatttat cgattggcag
     1081 actctggcaa agagcagcta gtgcggatct tgaaccctgg tgatttcacc ggagaatgga
     1141 ccttgttcaa cccggatgca gtacacgagg aatatgccga agcaacccga gataccgttg
     1201 tctgtatgat tcagcaaaaa gatgtccaag attttctgga acaataccca gctatttctt
     1261 taaaactatt ggcggaaatg tctaaacggt tagaaagttc agaacgtcaa acgacacaag
     1321 tagccgtgga gagtgtcatt acccgtttag ttttgttttt ggcagaaaat gtggaacctg
     1381 aaatgggcaa ctctcccacc atcaccctgc cgatggcaaa aaaggacatt gcttcctatt
     1441 taggaacgac acctgagacc atcagtcgca aatttggaga attggaggac gaaggattga
     1501 ttcaacagct gtctgggaaa aggatcaaga ttcaggattt agatgattta ttgctttaca
     1561 gcgaataatc ggacacactt ttttctacac gttggtgttc tgtcgctctt cattaacaac
     1621 gggacagaac tcagtgcttt tgcttccatc tgtccgtttt tggctttttt aagacagaac
     1681 gaccttcatt cattggaaaa ataaaagcac aaacatgata accctcaagt ttttttgttt
     1741 tatttttctt taagataggg ttgtcgaaca aaacgtaaaa ataagtaggg tgaatgtgca
     1801 ttgtttttgg gtgatagttg ccatcacgtt gtcagcagcg ggggcattct ttgtcgtccc
     1861 tttcaccaag aacacgattg gcggcgaagc aaaaacgtct aacgttgttg aggcagatag
     1921 gttagctatg gaaaaagaag aggaagttgt aaaaggagcc ggcgtaatcg gacacggaaa
     1981 agtggcgtat tacgaagcga gtgaccaggg agataaaagt gtattcactg tttggtttcc
     2041 atggagctta gccggaatct tgttcagcat caccatgact agaatgacgc gaaaaattga
     2101 agaaatggag caaatagaat gggattcgta ttaggcgttt tgaatcccca tgtggtggag
     2161 attggttctg ttgtgattca ttggtatgga gtcattattg cagcgggcat attagcggct
     2221 ctccagttga gcaccaagga agcaaagaaa aaaggactgg aagaggatac cattatcgat
     2281 atggccttat gggcaattcc gattggactc ctcggcgctc ggctttatta tgtgttattt
     2341 gaacttggtt attatctgca aaatccagga caaatccttg cgatttggaa tggcggcatt
     2401 gcgatttatg ggggattgat tgcaggaggg ggtacgctat attggtatgc aaagaaaaaa
     2461 ggtacttctt tggcactcgt tcttgatatt ttagcgccca atgtattgtt agcccagtcc
     2521 attggtcgtt ggggcaattt tatgaatcag gaagctcatg gtgggccggt tactcgtcaa
     2581 ttcttggaaa atctttattt acctgaattt atcattgaac aaatgaatat gaatggtacc
     2641 tactaccatc cgactttttt atacgagtca ttgtggagtt tgttgggctt tgtcctcatc
     2701 gtcactatac ggaaccgaaa acagcttttg cgtcaaggag aagtcgcttt gagttacgtt
     2761 ttgtggtact cagtgggtcg gttcttcatt gaaggtatgc gaacggacag tttatggatt
     2821 ggcgagtgga tccgcgtttc gcaagccttg tctctggccc tgtttattgg cgcgattgta
     2881 gtatggttta tacgcagaca agattatcca ccaatttctt attattctga tggcaataag
     2941 gggcagaaaa agactattaa gatggtgaat tgagcaaaaa gataaaaaga agtgcaaaac
     3001 ttgatatgcg tcaattttcg tttgctaaaa gtgagctata ctacagttgt aaagaggaaa
     3061 aggcagtgtt cattaatctg ctgccgataa aataaaaaca cataaaggag ataggaatta
     3121 tggaaaaagc tatattgcaa ttagaaacgt tgtcttgtcc aagttgtatg caaaaaattg
     3181 aaggtgccgt gaaatcagtt aacggtattg ataaagacag cattaaagta ttattcaact
     3241 ccagcaaagt caaaaccaat tttgattcag ctgtcacttc gattgaagag attcaaaaag
     3301 ctatcgaaaa cgtaggctat ccggttctta aagcaaaagt caaggccgtt taacagatta
     3361 aatgaactag gcaattctgg aagaaaagct atttttccag gattgctttt tttgttgcaa
     3421 tccgattcga ggtgcttaga aacgataaaa gagaactttc taaacttgat gtcagtcaat
     3481 taattactta ggcaattatc ttatactgtg tttatcaata aaagagaaat taaatttgga
     3541 gggaaaaagc atgcaacaat atatattaag caagaaaaat gttatcaccg tcatcagtgg
     3601 attgttaatc gtactcggtt tcttcagtca ctttgtttta gaaaacgtag gactttcaga
     3661 gtggtctttg atcatcgcct ctgtctttgg gattacgcca attgccattc aagcgtttca
     3721 agcaatgaaa gtcaaagtca tcagtattga tgtcttagtc agtattgcag cgattggtgc
     3781 gctttttatt caaaattatg aagaatcggc tattgtcacg ttcttattct tattcggaca
     3841 ctacttggaa caacgaacat tgaaccaaac gcgatctgct atcaaagaat tgactgaaat
     3901 ggcacccgaa agcgccttga aacaaatgga taatggcgaa tttgaagaag tagaagtgga
     3961 tgatatagat gaaggggata tcttgctcgt taaaacgggt gcgaaagttc cagtagatgg
     4021 catagtcctt acgggtgaag ggcatatcaa tgaagctagc attacaggtg aagctattcc
     4081 ggtcaacaag ctagctgatg cagaagtttt tgcaggaacc attttagaaa acggaacgat
     4141 tcaaatcaga gctgaccgag ttggtgagga caccacattt ggaaaaatta ttgaactcgt
     4201 ggaagaagca caagattcta aatccgaagc ggaacggttc attgatcgct tttctaaata
     4261 ctacacacca gctgtcttgg ttctggcaat tgttgtatgg gcgttcagtc aaaatattga
     4321 gttagcaatc accatcttgg ttctaggttg cccaggcgca ttagttatcg gagtgcctgt
     4381 ctcaaacgtt gccggtattg ggaatggtgc tcgtaacggt gttcttttaa aagggagtga
     4441 agtcattcaa gacttcagta atgtggatac aattgtattt gataagacag gtactttaac
     4501 tgtcggaaac ccaaccgttg cagcgactga actgtatgaa aaagatcctg ctgaaacgct
     4561 tggctatctg gccagtgtgg aacgggaatc agatcatcca ttagcaaaag cggtcttaaa
     4621 tcaaattgga gaaacaaact tttatcctgt tgaaggaact gaagttgtga aaggcggcgg
     4681 aatcgtttca agtgtagctg gacatagagt ggctgttggg aatgtggcct tgatggaaaa
     4741 agaaaatgtc actctcagca aaaaagtgca aaaagatgtc aaacggtttg aacaacaagg
     4801 gaactctctc gttttgacag cggtcgacgg tgaattaaaa gtactgatgg gcattcgcga
     4861 tcaagtccgt ccgggtgtga aagctaattt gcaggaatta aaagacttgg gcgtgaaaaa
     4921 tctagtcgtt ctttcaggag ataaccaagg aaccgttgat gtggtcgccg gcgaacttgg
     4981 tttgaccgaa gctcacggct acatgttgcc ggaagacaaa tcggcttata tcggaaaact
     5041 tcaacaacgt ggtcaaattg tagcctttgt tggagatggc gttaacgata gtccgtcctt
     5101 agctttagca gacattggaa tcgcaatggg aagcggaacg gacgtagcga ttgaaacatc
     5161 cgatgtcgtt ttaatgaact cgaacttcag caacttgcct cacgcattag gattagtaaa
     5221 agccaccgca aataatatga aacaaaatat cgtgatttca attggagtag tgttggtctt
     5281 gttgaccagc gtcttcttca gcgaatggat gaatatgtct atcggaatgt tggttcatga
     5341 aggtagtatc ttggtggtaa ttttcaatgg catgagatta atgaagtata agttgaaagg
     5401 tcgaaaagaa caaaaagaag tatcagcacc tgcagagaaa gtaaaaatat cttaagaata
     5461 aattagaaga ggctgagaca atagtccatc cttgaaatga caaaagcgga aactcagatt
     5521 tttaaatctg ggattttccg cttttttgag ttcttgaaaa aaaataagag tatagagtat
     5581 gttctttata aaattaattt tcgtgtaaat gattaaaaaa tttaataata aaatttaaat
     5641 tcctattaag ttgtaacaca tgaaattgct tataatagtg tattgaaatt aaggtagatt
     5701 gacaaacgag tgatcaaata gatatactac cttcaaacga ccgtttgaat gtgaggtgag
     5761 atgaatggaa aaagtttgcc aagtgactat cgtacataaa gaaaaagtaa atgtaataaa
     5821 aaaaaaattg gatcaaaaag atttttcaaa cttacttgtg ttagggaaat gtttcagcga
     5881 ttcttctagg attaaaattt tctatgcttt agaaacctat aaggaaatgt gtgtctgtga
     5941 tttagcagaa atacttactg ctagtgtcgc tacaacgtca caccatttac gttttttaaa
     6001 aaaacatgga atggcaaaat cacgtcaaga tggaaaagta gtttattatt ctttagcgaa
     6061 tgaagacatc ctttccgcta ttcgtgtttt tttaaagtta tcagaaaatc tatctatgaa
     6121 atcttagttt ggaggaagtt attttgttac aaagtatttt ttcagctatc gctgtttata
     6181 tttctaccag tattgattat ttgtttatct tgctgattat cttttctcaa agccacacaa
     6241 aaaaaggtat tcgtcacatt ttttggggtc aatatcttgg aacaggtatt ttggtagccg
     6301 taagtttgtt tgctgcttat gtgctaaatt ttattccgca agattggatc attggtcttc
     6361 tcggtctaat tccgattttc ttaggaatca gagtcgcttt tgttggcgaa gaggaggaag
     6421 aagaagagga agtcgttgaa aaactcgaat ctagaggaac taatcgctta ttctggacag
     6481 ttgccttaat aacgattgct tctggaggcg ataatttagg tatttatatt ccatacttta
     6541 cctcgttagc tttttcagaa atcattattg ctttaatcgt attcgctatc tctgttgcgg
     6601 ttctctgcta tatcagttac aaattagcca agatttcttt tgtttccgaa acgttagaaa
     6661 aatacgaacg aatcattgtc cctataatgt ttattgcgtt aggaattttt attatgatag
     6721 agaacgggac gatacaaacg ctactaggtt tttaaaaatc ataatcacga aagataaaaa
     6781 gagaggcagc gactaatcaa aatcgctgcc ttttttgctt ttcaaataat gaagtatttt
     6841 ttagcgtaga actatcttac gtgttgtggg agtatccctt ttcaagctct tctttgcttg
     6901 tagttatttc tattgtgata tgattctcta cgcttgattt gtgtgcgact tctgcaattt
     6961 cttcttttat ttccctagcc ttgtctatgt ctgaagggtc tataagaacc gtgatgataa
     7021 gggcgttttc ttccccgtcc agtgaccaaa tgtgaaaatg tgagaggtct tttacagcag
     7081 gaacattttt aatggagtcc gccaagctct ttacgtttac attttcaggc gaaccattca
     7141 ataaaatttt cattgtggtg aaaaattcag gaacggtttt gtagaggata taaagtgcaa
     7201 tcaaaatcga taagatagga tccagacgat atgcctcggt aaagttcatt agaatactca
     7261 caatcaagac tccaatccac cctaatacgt cttccagcat atggaggttc aacatgctct
     7321 cgtttttgga tgtgcctttg cttaaaatcc acgcagcata tccattcaat gcaatagcta
     7381 cgagagaaag ccagaacatg ccactataat ttacgggttg tgggtcaatc aaacgtggca
     7441 cactacgata gatcataaaa aacgagccac taattagcac aacgccagtt atcagcgcgc
     7501 ccagtaaaga aaagcggtta tagccaaaag tgaaccgttc atcttgtttt ttctctgaat
     7561 aaccttgaaa aaaccaagcg aatccaattg aaatagagtc ccctaaatca tgaactgcgt
     7621 cggacataat ggacacacta ttgaaaagaa agccaaagaa aaattcgctt atagagaaaa
     7681 taagatttat gaagaaagca attttaatat tctttttcgg ttgattctga ttcgagtgat
     7741 tatggtcttt atctgatttt gacatttaaa aacctcctaa gtttgtatgc tacatactaa
     7801 ttggttttat ttttctaatt cattaacatg agttaagact tgttcgagaa tactaaaaac
     7861 atgaaggtca tctaaactgt aaagcatact ttttccttct cttcttgatt tcactaagcg
     7921 tgcgtctttc aacgttttta attgatgaga aatagcggac tgttccatgt ccagagaaat
     7981 agcgatattt ccaacactac actctttttt ctgtaaaaga aagagaaccg acaatcgtgt
     8041 aggatcacta atcaccttaa atagtttgct tattgacaga atagatcctt tatcgattgg
     8101 agaagttgtt tttatatcca tagtgttttt attcctttca tatgaccttc cgttcatatc
     8161 tatattacca tatgagcgaa acaatgctaa tcttttgttt ataaaagaga agtggcgttc
     8221 tctttatctt gacaaaatgt atatagcgat ataaaatgag tatacaaaca tatgattaat
     8281 tagtcatatg taaggtggat tcattacgat ttacttttgg aataaacgaa aggagtcaga
     8341 aatagcagaa atttatgtga ttcaagaagt aaatgatgga aaacaaagga attataaatt
     8401 atatagggag gcaatagaag tggaaactat ccaagaacaa cagtctcaag aaaagcataa
     8461 tcataatcac aaccacgatc acgaccatga tcatgggaaa atgccagtcg ttttgtactt
     8521 tgttggttta gcattagcag tgattgctct atttttgaat gaagattatc agttggtaca
     8581 aaatgttttg ttctcaatcg ctacaatcag tgccgggtat cacgttattc ttcttgaagg
     8641 ccttggagaa acaattgaga attcaaaagc acaaaaaaag ttcataccta attctcatat
     8701 tttaatggga ttagcggcaa ttggagcttc tttgatgggg aatttttggg aaggaacatt
     8761 attgattctt attttttctg gcgcacattt tttggaagat tatgcagaag gaagaagcaa
     8821 acgagaaata acgaaattac tcgaaatgaa tccaacgact gcccggttaa tcctgcctga
     8881 tggtagtaca aaaaatgtgg atgtcagtga attaaaagtg ggagatcaac ttcaagtgtt
     8941 gaatggcgat caagtaccta ttgatggcgt cattttgtct ggatctacat ccattgatga
     9001 gtcgtctatt actggagaaa gtatcccgaa agagaaatcc aaaggagatg gcgtcttcgg
     9061 gagtacaatc aatggaacag gtaccttcac gatgaaagtc accaaagaaa acaaagatac
     9121 agtattttca aaaattttac aattagtgaa tcagaaccaa gataaccaaa caaaagctgc
     9181 gagtattatc caaaaatttg aaccgaaata tgtcacagtt gttttaattg ccattcctct
     9241 attcatgtta ttggctcctt ttctacttga ttggacatgg tcacaaagtg tctatagagg
     9301 attggtcctt ttagttgcag cttcaccgtg tgctttagca gcagctactg tctcggcaac
     9361 gctatcgacg acttctaatt tagccaaaaa aggagtcctt tcaaaaggta gttcttacct
     9421 gtctcaatta gccgatatta aagcaattgc atttgataag acgggcacct tgaccaaagg
     9481 aaaacctgaa gtaaccaatt attattttgc tgattccgtg aacgaagaaa acatgattga
     9541 tatcgtagta gctcttgaaa aagaatccaa tcacccctta gcagatgcta ttctaagaaa
     9601 gtttgagcaa aaaaacaaac taactattga agtagaaaac caaattggta aaggattgac
     9661 gggagattac aaagggagaa attatcgtat aggaaaaccg acttcgtttg atgatgtttc
     9721 agatgaatat agtcgtttaa ataatgaatg ggcgtcagaa ggtaagacag tcgtatacgt
     9781 agcagtagat gaagaggtta taggccttat tgcccttatg gacgttccga gtgaatatgc
     9841 taaagaaacc attgaatatt ttaggaaaca aggtatacac accacattaa ttactggtga
     9901 ctcagaaatg acgggaaaag cggttgctaa acaattagga atagatgaag tgattgcgaa
     9961 tgtcatgccg gaagacaaat ccagaattat agacgaacaa aaagaaaaat acggcgtgac
    10021 cgccatggtt ggagacggtg taaatgatgc tcctgctctt gttaatgcag atgtgggaat
    10081 cgctatggga gatgggactg atgtggcagt agaggtatct gatttagttt taatgcaaaa
    10141 caatttatct aaattggtac aatctcataa tatttcttcg aaaatgaacc gtgttatttg
    10201 gcagaatatt attttttcaa tggccgttgt tgctttttta attgtcgtta gtttcttagg
    10261 cttaacggat attgcaatca gtgtcattat tcatgaagga agtactttgg ttgttatttt
    10321 gaatggactt cgattgctaa aggcagtcta aagaatgatt gcacaacttc aaattaatcg
    10381 agtaatagag ataattaagg attacactac attttttatc tctattactt gtttatattt
    10441 atctagatgt tcaaagtcaa aatgatatat caaccatact cttgtggcgc gggttgaata
    10501 taagatacat gaaattggat aaaatcatgt atcaagaatc ctatccttat agggttcttt
    10561 ttttttactc cccctaaaca cacgatttcg tgttgtaata actataatca tgtattcgaa
    10621 cagactaaaa aggagggatt gtcatgggct ataatgttca gccactacgg tcgcaacagg
    10681 agattaatga ttttttattt tgcctgcgac ggaataaaaa tgcggaacgt gacgttttct
    10741 tgtttctaat tggaattaac agcggtctgc gcatgtccga catcgttaaa ctcaagaaaa
    10801 aagacgtgat tacctcaaaa aacccgcgca tcgtggagca aaagacaggg aaaacacgca
    10861 ttttatactt aagcagctta caggacttga ttcacggcta taccgcaccc ttagatccgg
    10921 aggcttactt gtttcccagt actaagggcg gccatttaga agtcaatacg gtctaccaga
    10981 tgtttcaaaa ggtcgcgaag ctgttaggaa gggacgatat cggcacccac accctacgaa
    11041 aaacctttgg ttaccactat tacaagaaaa ccaaagacgt ggccacctta atggaaatct
    11101 ttggtcacag cagcgagaag attaccaaac gctacatcgg aattaatgaa gatgaaatca
    11161 gtgaaacgtt attaaatttt cggttaggtt tttgattgta ttatataata gataagcaca
    11221 gtaagacggt tataatcacc cacagtattg gacaaatcaa cctttttttg gtatattata
    11281 tatacaaatg agagcaggct catagtcaac ttgatataaa tccggaacat aagggtccgg
    11341 cgggtggcga gcaccaaaac taaactcgat acaaatccgg aacgtaaggg tccggcgggt
    11401 ggcaagcacc aaaactaaac ctgtacaaaa ggctgctctt ttgagcagct ttttttattg
    11461 ggaaaaagga gcattcttcg cacgcggcaa aaaaagaata caaacgcatg tctataaaag
    11521 agacaaccca aattaccaga cagttaaacg cgatttataa ggctgctcat ctcttacaag
    11581 aacattttgt agataaaaaa gtatcctttg tcggagaagt ctcaacggta gcgattattt
    11641 tttcgacaac taattttatg cacctctgtg gcattgatta tcgcagaggc acacacttgt
    11701 tttttcaaga tgccttagac aggaaaataa atttacagga tattcaaatc aaaacggatg
    11761 ggacgacctt tcagaaatta caagtaatag gtagccttga tttattattg gggaagcata
    11821 tttctattgt tggcagaggt gtttactctt ctcttcgcta tgatgcagct attcgaacaa
    11881 ggaaaaaaat attggctctg tctttgaaac aaaacggatt aatctatatc cctatctctt
    11941 tgttaaacct atcatcaaag gaaattggac ctggacaaaa agttactggc atttttagcg
    12001 aggatttaac atccggggaa cttaaaatga ttatggaagt catcgattaa tcattaatag
    12061 tgatatgcga cgtcattttg aagtaattat tctattttgg gttataaaat aaagcatttt
    12121 aagacgattt caaatcattc atgtgggtta tttatcacgg atgagcttga aatcgtcttt
    12181 tatacgtatc aaattgaaat gttcggcata caacctatcg ttgttactac attactaatt
    12241 gtcacttgtt aaggaacttg gatcctttct aaacattccc tagagataaa ttttacttta
    12301 tccctccgtt aatatccaat ttaagcacct ttccgattca gggttaattt gcatatcaag
    12361 tgcagcttgt gttctagtaa gattagccct tttagtccgg ctgagttgac ttatggatat
    12421 aagatatggt catagcaaat agatactacg tatagtctgt taatttagtg tcattaatct
    12481 taa
//
LOCUS       DAZKLD010000088        12467 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.088, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000088 DAZKLD010000000
VERSION     DAZKLD010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12467)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12467)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12467
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.088"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            45..518
                     /locus_tag="ACG8KR_001934"
     CDS             45..518
                     /locus_tag="ACG8KR_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HFQ0201295.1"
                     /translation="MKFKTLLLKFVVLGISAVFILFGVLFYTQIATSEKGFNFDWQTT
                     LFVSVIYFALLLGIVIAYFLLKLLKKIEDNQAFSTQSLNILKKIERTILFIGFAFFGL
                     LPKFMKVADLDDAPGLMLVGIAVVFIPFAVYTLTSVLEKLLEHAILLKTDHDLTV"
     gene            527..754
                     /locus_tag="ACG8KR_001935"
     CDS             527..754
                     /locus_tag="ACG8KR_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0201296.1"
                     /translation="MAIIVNLDVVLAQKKMSVTELSEKVGITMANISILKNGKAKAIR
                     FSTLEKICQVLECQPGDLLTYKNDSNTIPEE"
     gene            1390..1761
                     /locus_tag="ACG8KR_001936"
     CDS             1390..1761
                     /locus_tag="ACG8KR_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0201297.1"
                     /translation="MPFIVYFFLSLLVIYIPLPTFFLLVNRLAEQHDTIAALTKLCLL
                     LFVCLDYFTSYYWLKQTRVKKRYYFFLVFFNLFEFLIHCFLLHQYHTIHLWIIWVFKV
                     VVLFCMFTFPISRKFREALFY"
     gene            complement(2017..2259)
                     /locus_tag="ACG8KR_001937"
     CDS             complement(2017..2259)
                     /locus_tag="ACG8KR_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFQ0201298.1"
                     /translation="MGFIWSLIVGGIIGAIAGAITNKGSSMGIIANVVAGLVGSALGQ
                     ALLGSWGPSIAGMAIVPSLIGAIILVLIVSFFARKL"
     gene            complement(2291..3193)
                     /locus_tag="ACG8KR_001938"
     CDS             complement(2291..3193)
                     /locus_tag="ACG8KR_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFQ0201299.1"
                     /translation="MNNNNKNNNNKMNNQKPVSAHPDPTTPNQSNGVNDEKNKTAPVS
                     AHPDPVVPNVQQGVEEKKHDAEKHAPHHPGTPESKVPEQHDAIDPSKPVEEQKHPSDH
                     AKAQEKLSSDHTNGQGKLGSDHTNNQGKHDSEQEDSMRGELNYEDKVVQKIIGIAIEQ
                     VDGLLSANGGFFSNVAGKLVNTDNVTAGIETEVGKKQVAVDMDVIVEYGKDIEKIFEE
                     MQEVIGKEVKNMTHLEVIEVNANVVDIKTKEEFEKDQETVQDKVTDAAKKTGEFASNQ
                     TDKVKSAAGSGAQKVKENTEPRVQ"
     gene            complement(3206..3394)
                     /locus_tag="ACG8KR_001939"
     CDS             complement(3206..3394)
                     /locus_tag="ACG8KR_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HFQ0201300.1"
                     /translation="MKELFSTYKLPIIFGVLGLILAVLWITVGFFKTLLILIMAAIGV
                     AIGFYLKQTKILDHYFKS"
     gene            complement(3408..3971)
                     /gene="amaP"
                     /locus_tag="ACG8KR_001940"
     CDS             complement(3408..3971)
                     /gene="amaP"
                     /locus_tag="ACG8KR_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HFQ0201301.1"
                     /translation="MNKGVKTIGVIGTLLLLSVLLFAILLSATYPLPFQLEKFRFFSI
                     TNSYVQQYVFWLAVAFAILTIITLLVFLFYPKTIGNFLLKHGDGELTLDKKAIEGMVR
                     SHLHEDEFIHSPKVNVKATKNKIAVNVKGELKRTSALIGKTGTLMKEIENEVQHILGT
                     KEPVKVSVKYTGFEEPAKRSNDRKRVE"
     gene            complement(4009..4902)
                     /locus_tag="ACG8KR_001941"
     CDS             complement(4009..4902)
                     /locus_tag="ACG8KR_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFQ0201302.1"
                     /translation="MTEPKLIDPRNLYHTEKFPKQDQDTPALQQNMIPVPDCGEESYE
                     GNNQLENRRVLITGGDSGIGRAAAIAFAREGADIALQFFPGEEKDAEEVAKYVKVAGR
                     KIVLLPFDLRDEQAPKEIIDKAVSELGGLDTMVLNAGQQISCQSIAELPLEQVKDTFM
                     INIIAMFALVKEAVPHIPAGGAIVTTTSVQAFNPSEHLLDYAATKASIANFTIGLAKQ
                     LAPKGIRVNGVAPGPIWTPLQLDEGQPSDSIPEFGQQSLLERAGQPGELAPVYVFLAS
                     NKASYVTAQIYGVTGGEAINL"
     gene            complement(4980..5903)
                     /locus_tag="ACG8KR_001942"
     CDS             complement(4980..5903)
                     /locus_tag="ACG8KR_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HFQ0201303.1"
                     /translation="MDEEKNVIETDEKKYNWLVLDSAKPEEIEQAIEEFELPDDIFVG
                     TEYPEEVSRMERLYETKLEHPFSLVVINLDQSDNRIEKKLTPISFVLSDNLLITCIDR
                     KTEFVDRLLTKHGNHLNSFEKIITYTLLDIYTHYVKELREMKKRIDALDKAARKTTEN
                     EELYKQADLERDIVYIDHTLRDQRETMDQLWNSETFIKRLDDEQLLYDVHLRQRQTEK
                     MIEIYRDLLESIGDLFNGMMDNNLNHIMKYLDSAALIISVPALFAGIWGMNTGGLPGK
                     SSALGFILVVFGSLIAAVVLGYHLYRKDYTN"
     gene            complement(5952..6302)
                     /locus_tag="ACG8KR_001943"
     CDS             complement(5952..6302)
                     /locus_tag="ACG8KR_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HFQ0201304.1"
                     /translation="MEITMSKNAVETLIEKVGENTKIALALINDSDPFLRDKGAFAKG
                     SFFQIIPFVSEFGEYATKIEHPLLDIYTSKLEQNYFGKRLNMDFNKQLDSFSLENEIA
                     VLDYNIKLKNCFFS"
     gene            complement(6335..7237)
                     /locus_tag="ACG8KR_001944"
     CDS             complement(6335..7237)
                     /locus_tag="ACG8KR_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HFQ0201305.1"
                     /translation="MNKKEIAVVASCNTKFVPHLAALFVSVLDNCNPSKFVRFYVIDD
                     DIDFESKQLLRFSVKNARMNSDVEFLKINKEFFTNVVISDRIPETAYYRIAIPELFRG
                     TEVERILYMDCDMIALQDISKLWRLDFGDSIVAAVEDAGFHQRLEKMKISAKSMRYFN
                     SGLMLINVKKWLDENITQKVLDFIEHNPEKLRFHDQDALNAILHDRWLPLHPRWNAQG
                     YIMAKAKKHPTAAGEREYEETRNNPYIIHFSGHVKPWSKDFEGPTKKYYEKYAGMTAF
                     RCVAKFPKYPKYAKVQPKTDVVVK"
     gene            complement(7230..8087)
                     /locus_tag="ACG8KR_001945"
     CDS             complement(7230..8087)
                     /locus_tag="ACG8KR_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HFQ0201306.1"
                     /translation="MEKRYGVVPVVTASDENYAPYLSVMIATALENCNKTRRIKFYVI
                     DDGLSEYSKEGLEETVNKYSSNASIQFLTVEKDIYEDFLVSDHITTTAYLRISLPNLL
                     AKEDYKKVLYLDSDVLVLDDIVKLYDEPLNGKTIGAIIDPGQVKALERLGIDSDDLYF
                     NSGVMVIDIDQWNKKEITEKTIHYLSENGDRIIYHDQDALNAVLYEDWEQLHPKWNMQ
                     TSLIFERHPAPNEKYERQYKEGNEKPSIVHFTGHDKPWNTLKDHPYTNLYLKKLAHST
                     LTKVGEINE"
     gene            8421..9230
                     /locus_tag="ACG8KR_001946"
     CDS             8421..9230
                     /locus_tag="ACG8KR_001946"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFQ0201307.1"
                     /translation="MKLAAIDLDGTLLDRNGKIPEENIQALRKFDENGGIVTVATGRN
                     SISAKDIFSELGIGGYLISSNGSLIAEMKDGKMAKVLRRSKIDSDTLRKAFFYAQEAK
                     ISIIASRETQDDQLTFDESNLVKDDPYYEHFNLQNHSFDQILNMLEDPSLSYLKLALT
                     DKSEEKLQKLQRDLQADGIQSVFSDPHFLEITPTGITKAHSLVFLADYLGIESSDIMA
                     FGDQENDIAMLNYSGLSIAMGNAQRQVKEIANQVTLTNEEAGVAYILKNFY"
     gene            9270..9767
                     /locus_tag="ACG8KR_001947"
     CDS             9270..9767
                     /locus_tag="ACG8KR_001947"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HFQ0201308.1"
                     /translation="MLVIIDMQNHILDPNSEFYIPESDQLAERIAQRLAKARQNNEYI
                     LFTRDIPVELKDKEEEREDLQLIPLLSPQENERVIKKYYFTIPPETLAEIQETLFESK
                     EEQKEIEVVGIETNLCVLSNLIGLQSAFPEADFFVDPMLVSSRKHGESALELLKDFNV
                     SIVES"
     gene            complement(10152..10225)
                     /locus_tag="ACG8KR_001948"
     tRNA            complement(10152..10225)
                     /locus_tag="ACG8KR_001948"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10189..10191),aa:Ile,seq:cat)
     gene            10412..10735
                     /locus_tag="ACG8KR_001949"
     CDS             10412..10735
                     /locus_tag="ACG8KR_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0201309.1"
                     /translation="MLGVIILPFIAILFDLVAAAAYFLQLNYQTSGILFIGMIFQGVI
                     TLLLLVMMLSYKGKRYARIQTKVFVKYVSIRYGIIILSFLVNAIVLFLYVLNYLDINP
                     LIFSR"
     gene            10899..11153
                     /locus_tag="ACG8KR_001950"
     CDS             10899..11153
                     /locus_tag="ACG8KR_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0201310.1"
                     /translation="MITLVRVLFWVPAVALVASIVYLMNWNKERFYLAILTLPAIYFM
                     WKVFNYNYFEPDSVFIEELSGLVLSLLIVILYLIRLNKKH"
     gene            11223..11468
                     /locus_tag="ACG8KR_001951"
     CDS             11223..11468
                     /locus_tag="ACG8KR_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFQ0201311.1"
                     /translation="MSIEEAILLDTLRCRLNFLRTGRSLSQVAVSSALSIPLFKYRKY
                     EEGEEIPSIIELIYIADFYDISVDYLIGRSETASKLK"
     gene            11544..11909
                     /locus_tag="ACG8KR_001952"
     CDS             11544..11909
                     /locus_tag="ACG8KR_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02328 family protein"
                     /protein_id="HFQ0201312.1"
                     /translation="MRLWHEKLISKLPRQQLLGQHRECCALRGNGWGKKHATVDYVFH
                     YSPYRLFQYHQLVMDEMEKRGYSPAPEWKNPMYRGKSCEAFFDLPTVERNVPIYPEHD
                     EVYLAECLANLEQKGIYLT"
     gene            complement(11970..>12467)
                     /locus_tag="ACG8KR_001953"
     CDS             complement(11970..>12467)
                     /locus_tag="ACG8KR_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0201313.1"
                     /translation="FRLKTKKESVIILVYFQGGNLMIKKLFLLSILTLAMAGCSDSGL
                     ATELITKSTEATTEELKCGNDHSDYRGLNRRCMGILGRSNCKHRCSNRRRCRRSNRKI
                     KRKIKRSNLCCASYTDVEDAVLLNHVRRCSFMSREVGDEPKLEYAFSFAREDEDNPKT
                     YDILA"
BASE COUNT         3587 a         2327 c         2134 g         4419 t
ORIGIN      
        1 tattctgcta tattttagct atcaaagata aaagagggat aaaaatgaaa tttaaaacat
       61 tgctattaaa atttgtcgtt ttaggtataa gtgccgtatt tattttgttc ggcgtacttt
      121 tttatactca gatcgctaca agcgaaaaag gattcaattt tgattggcag accacattat
      181 ttgtttctgt catttacttt gcccttctcc tagggatcgt tattgcttat ttcttattga
      241 aactattgaa aaaaatcgaa gataaccaag ctttttccac tcaaagctta aacatactaa
      301 aaaaaatcga acgaaccatt ttgtttattg gtttcgcctt ttttggctta cttcctaaat
      361 ttatgaaagt agcggattta gacgatgctc caggtttgat gttggtagga atcgctgtcg
      421 tattcattcc ttttgcagta tacacattaa caagcgtgct ggaaaaactg cttgaacatg
      481 cgattttact taagacagat catgatttga cagtgtgagg tgcgacatgg caattatcgt
      541 taatttagat gtagtattag ctcaaaaaaa aatgagtgtg actgaattga gtgagaaagt
      601 gggcattacg atggcaaaca tctctattct aaaaaatgga aaagcaaaag ccatccgttt
      661 ttccactctc gaaaaaatct gccaagtttt ggagtgtcaa ccaggtgatt tgttgaccta
      721 taaaaatgac tcgaatacta taccagaaga ataaaataaa gtagaacaaa cagtaaataa
      781 aaaataatct gaatgataga tgtgtgagac ttttagaagt tatttacgtc tattattcag
      841 attttttatt tcgattgaat accgttttat ctataaataa gatatgtact gactcataca
      901 aagggggcct tttcttctcc atgtactatc acttttcctg atacaactgg tttcctgtta
      961 cgttgtagtt ggatctgctt ttagtataat actttattca ttccaccaga ctgatagcag
     1021 tttgagcagt ctaaataatc atgtagtatt acaggccatt ctgtttcata ccttgaagtg
     1081 cttaaaactt cagctaatgg cccattgtgt taactcttac tccattcttt tttagtaagc
     1141 tcccatatat actgtaaagc cttctatata ttatgtcatg ttgcttttta acatttacta
     1201 tgtagttttg ataaaatgat ttgaataaca gtgatggaca attctttttt atttttaaca
     1261 ttattttgat aataatgatt atttatttaa aaatacatta gataacattt gttacgacac
     1321 gaaaaagctt cacaaacaaa tatcgttatt ttaaaatgag ggcgaactca cacaattagg
     1381 aggaatagta tgccatttat tgtttacttt tttctttcat tactcgtgat ctatattcca
     1441 ttaccaacat ttttcctgct cgtcaatcgt ctagcagagc aacatgatac gattgctgct
     1501 cttacgaaac tttgtttact tctatttgtt tgtctagatt actttacgag ctattattgg
     1561 ctaaaacaga caagagtaaa aaagagatat tatttcttcc ttgtcttttt caatctcttt
     1621 gagtttctga tccattgctt tttacttcat cagtatcata cgatccattt gtggatcatc
     1681 tgggttttca aagtggtcgt attattttgt atgttcacct ttcctatctc aagaaaattt
     1741 agggaggcat tgttttatta aagaagcttc tctgtatcaa taaagacaca tttaaggaaa
     1801 aacttttaaa aatctaattg ggaatttgcc cgcataaata agaggctgtg acaaaagtct
     1861 tgtcacagcc tcttattccg tataaacagt gttcaaaagc ttgcatcttc gataatatgc
     1921 ccaaccaaat gaaaaatatg agaagctatt tttgtttggc tgttcttatt acttgatgaa
     1981 gaacagcttt ttcacgacct ctttttaaaa gtctttttac agttttcgag caaagaatga
     2041 tacgattagt actaaaatga tggcaccaat caatgatggc acgattgcca ttcctgcaat
     2101 acttggtccc caagaaccta ataatgcttg accaagtgct gatccaacta aacctgctac
     2161 gacatttgca ataatcccca tcgaagaacc tttattcgtg attgctcctg caattgctcc
     2221 gataattcct ccgacgataa gtgaccaaat aaatcccatg tttcgttcct cctaatgttt
     2281 tacttcctta ttactgaacc cgtggttctg tattttcttt tactttttgt gcgccgcttc
     2341 cagctgctga ttttacttta tctgtttgat tcgaagcaaa ttcaccggtt tttttcgctg
     2401 cgtcagttac tttgtcttgc accgtttctt gatctttttc aaattcttct tttgttttga
     2461 tgtccactac gttagcgttg acttcgatca cttctaaatg tgtcatattt tttacttctt
     2521 tgccgattac ttcttgcatt tcttcaaaga ttttttcgat atctttgcca tattccacaa
     2581 tgacatccat atcaactgct acttgttttt taccgacttc tgtttcgatc ccagccgtta
     2641 cattatctgt attcacaagt tttcctgcca cattagagaa gaaaccaccg tttgcagata
     2701 ataaaccgtc tacttgttca attgcgattc cgatgatttt ttgcacaact ttatcttcat
     2761 aattcagctc accgcgcatt gaatcttcct gttcagaatc atgctttcct tggttatttg
     2821 tatgatcaga accaagtttt ccttgtccgt ttgtgtggtc agaactaagt ttttcttgtg
     2881 ctttcgcatg gtcagaagga tgtttttgct cttccacagg tttagaagga tcgattgcat
     2941 catgttgttc tggaacttta gattctggag ttcctggatg atgaggtgca tgcttttctg
     3001 catcatgttt cttctcttct actccttgtt gaacgttagg gactactgga tctggatgtg
     3061 cagatactgg tgctgttttg ttcttttcat cgttcacacc atttgattgg tttggtgtcg
     3121 ttgggtctgg atgtgcagac actggttttt ggttgttcat tttattgtta ttatttttgt
     3181 tattgttatt catggatgtt tcctcctaag atttaaaata gtgatctaag attttcgttt
     3241 gtttcagata aaaaccgata gcgacgccga tcgctgccat gatcaagatc aataaggtct
     3301 tgaaaaagcc cacggtgatc cataaaacgg ctaatatcaa tccaagtacc ccaaagatga
     3361 tagggagctt gtatgtcgaa aatagttctt tcatttttgg tcccccccta ttctactcgt
     3421 ttgcggtcat tgcttcgttt agctggttct tcaaagcctg tgtatttcac cgatactttc
     3481 actggctctt ttgttcccaa tatgtgttgt acttcatttt cgatttcttt catcaatgtt
     3541 cctgttttac caatgagtgc tgaagttcgt ttcaactccc cttttacatt gacggcgatc
     3601 ttgtttttcg ttgctttgac atttactttt ggtgaatgga tgaattcatc ttcatgcaag
     3661 tgagaacgaa ccattccttc aattgctttt ttatccaacg tcaactcccc atcaccatgt
     3721 ttcagtagaa agtttccgat tgtttttgga taaaacagga aaacaagtaa cgtgataatt
     3781 gtcaaaatag caaatgcaac ggcaagccaa aatacatatt gttgcacgta tgaattagtg
     3841 atggaaaaga atcgaaactt ctctagctga aaaggcaatg gataggttgc acttaatagt
     3901 atcgcaaaca aaagcacaga tagcaaaagt aatgttccga tcactccgat cgtttttaca
     3961 cctttattca ttttcgcacc tcctcatagg ggtgaaacga tcaacttctt ataagttgat
     4021 cgcttctcca ccagttactc cgtaaatttg ggctgtaaca taacttgctt tattcgatgc
     4081 taagaaaaca taaactggtg ctaattctcc tggctgacct gcacgttcta gtaaactttg
     4141 ctgaccaaat tcagggatcg aatcagaagg ttgtccttcg tctagttgta atggtgtcca
     4201 gattggacca ggtgctacac catttacacg gattccttta ggtgcaagct gttttgctaa
     4261 accgatcgta aagttagcga ttgaagcttt tgttgctgcg taatctaata aatgttcgct
     4321 tggattgaat gcttgaacag aagttgttgt tacgattgct cctcctgctg ggatatgcgg
     4381 aacagcttct ttcactaaag caaacatagc gatgatgttt atcataaatg tgtctttgac
     4441 ttgctcaagt ggtaactcag caatagactg acaagaaatt tgctgtcctg cgtttagtac
     4501 catcgtatct agcccgccaa gttcactcac ggctttatct atgatttctt taggtgcttg
     4561 ttcgtcacga agatcaaaag gtagtaagac aatcttacgt cctgcaactt tcacgtattt
     4621 tgctacttcc tctgcatctt tttcttctcc tgggaagaat tgaagagcga tatctgcccc
     4681 ttctctagca aatgcaatcg ctgctgcacg accgatccct gaatctccac cagtgattag
     4741 tacacggcgg ttttctagtt gattattgcc ttcataggat tcttctccac aatcaggaac
     4801 aggaatcata ttctgctgta aagctggtgt atcctgatct tgtttaggaa atttctccgt
     4861 atggtacaag tttcgcgggt cgattaattt tggttctgtc attgctgtat tcctcctagt
     4921 ttttaaattg tattgccaaa acaaatttct ttgtttgata cctgttttaa atagattctt
     4981 taatttgtat aatctttccg gtataaatgg taacctaaaa ctactgctgc tatcaaagag
     5041 ccaaagacta cgagtataaa gcctagagca gaacttttac ccggtaatcc tcctgtattc
     5101 attccccaaa tccctgcgaa caacgccgga acggaaatga ttaatgcagc tgaatctaga
     5161 tatttcatga tatgatttag attattgtcc atcatcccgt tgaatagatc accaatactc
     5221 tctaataaat ctcgatagat ctcgatcatt ttttctgttt gtcgctgtct caaatggaca
     5281 tcatagagta gctgttcatc gtctaaacgt ttgatgaaag tttcagaatt ccataattga
     5341 tccattgttt cccgttgatc tcttaaggta tgatcgatat atacgatgtc tctttcaaga
     5401 tctgcctgtt tatacagttc ttcattttcc gttgttttac gagctgcttt atccaaagcg
     5461 tcaattcgct ttttcatctc tcttagttct ttcacgtaat gggtgtaaat atctaataat
     5521 gtatacgtga tgatcttttc aaacgagttt aaatgatttc catgctttgt cagcagccga
     5581 tccacaaatt cggtttttct atcgatacaa gtaatcaaga gattatcgga aagtacaaaa
     5641 gatattggcg ttaatttttt ttcgatacga ttatctgact gatctagatt aatcactacc
     5701 aaagaaaatg gatgctctag tttcgtctca taaaggcgct ccatccgaga aacttcttcc
     5761 ggatattcgg ttcctacgaa aatatcatca ggaagctcga attcttcgat tgcttgctca
     5821 atctcttcgg gctttgcact atcaagtacg agccaattat attttttttc atctgtttct
     5881 attacattct tttcttcatc cacaaagtag tatctcaatg tatacctcct ttttggactt
     5941 tctcgaaact attagctgaa gaagcagttc tttaatttga tattataatc gagtactgcg
     6001 atttcatttt ctaatgaaaa gctgtcaagc tgtttgttga agtccatgtt caatcgttta
     6061 ccgaaataat tttgctctaa tttagatgta tagatgtcca ataatggatg ctcgatttta
     6121 gtcgcatatt ctccaaattc cgaaacaaaa ggaatgattt gaaaaaagct gccttttgca
     6181 aaagcacctt tgtcccttag aaatggatcg gaatcattga taagcgcgag tgcgattttt
     6241 gtattttccc caaccttttc aattaacgtc tctactgcat ttttagacat tgtaatttcc
     6301 attcctactc cccctcttca gttgttgtct tctattattt aacaactaca tctgtttttg
     6361 gttgaacttt tgcatatttt ggatatttag ggaatttagc tacacaacgg aaagccgtca
     6421 tccctgcata tttttcgtaa tatttcttcg ttggcccttc aaaatccttg ctccacggtt
     6481 tcacatgtcc tgaaaaatgg ataatgtaag gattgttgcg agtttcttcg tattcacgtt
     6541 cacctgctgc tgttggatgt ttttttgctt ttgccatgat atagccttgt gcattccaac
     6601 gcggatgaag tggtaaccaa cggtcatgta agatagcatt taatgcatct tggtcatgga
     6661 atcgtaattt ttctggatta tgttcgatga aatctaatac tttttgcgta atattttcat
     6721 ctaaccattt tttcacattg atcagcatca aacctgagtt gaaataacgc attgatttag
     6781 ctgaaatttt cattttttcc agtcgttgat ggaacccagc gtcttcaaca gctgctacga
     6841 ttgaatcacc gaagtcaaga cgccataatt tagagatatc ttgtaaagcg atcatgtcgc
     6901 aatccatata taagatacgt tcaacttctg tgccgcggaa caattccgga atcgcaatac
     6961 gataataagc tgtttctgga atacgatcgc tgattaccac atttgtaaag aattctttat
     7021 tgattttcaa gaattccaca tctgagttca ttcgtgcgtt tttcactgag aagcgtaaaa
     7081 gctgtttact ttcaaaatcg atatcatcgt caatcacata gaaccgaaca aatttagacg
     7141 ggttgcaatt atctaatact gagacaaata atgcagctaa atgtggtacg aattttgtgt
     7201 tacaacttgc gactactgct atttcttttt tattcattga tctcgcctac tttcgttaga
     7261 gtactgtgtg ctaatttttt taagtataaa ttggtatacg gatgatcttt caacgtattc
     7321 caaggtttat catgtcctgt aaagtgaacg atcgaaggtt tttcatttcc ttctttatac
     7381 tgacgttcat atttttcatt tggtgctgga tgacgttcaa aaatcagcga agtctgcatg
     7441 ttccattttg gatgaagttg ttcccaatct tcataaagta cagcattcaa agcatcttgg
     7501 tcgtgataga tgattcgatc gccattttca ctgaggtaat ggatagtttt ttctgtaatt
     7561 tcttttttat tccattgatc aatgtcaatc accatcactc ctgaattgaa gtacaagtca
     7621 tcggaatcga ttcctaatct ttccaaagct ttcacttggc ctggatcgat aatagcgccg
     7681 attgtttttc catttaacgg ctcatcatat agttttacga tatcgtctag tactaacaca
     7741 tctgaatcta agtaaaggac tttcttataa tcctctttag ctaataagtt tggtaaagaa
     7801 attcttaagt aagcggtagt tgtgatatgg tcactgacta ggaagtcttc gtagatatct
     7861 ttttccactg tcaggaattg gatacttgca ttacttgaat atttatttac cgtttcttcc
     7921 aatccttctt tactatattc tgatagacca tcgtcaatca cataaaattt tatgcgtctt
     7981 gttttattgc aattttctaa tgcagttgcg atcatcacgc tcaagtaagg agcataattt
     8041 tcgtctgaag cagttaccac aggaactacg ccgtatctct tctccatcaa aatcactcct
     8101 ttttctaatc tttttctaac ctgttctaca tctagaatat tcctcatttg gttttaaagc
     8161 aaaatatatg ctttgttttc attgaatatt ttaaaatttt tttatcacaa aacgcatcgg
     8221 taagcgctat ctattcgttt ttcatggctt tttattcaaa ggtattgacc aaaaactact
     8281 atatatagcc acctttttct taagaatgtt ttttgaaaaa attcataatg acaaagcaag
     8341 ttagtttcat ttttttaaat tctcatttaa tcttaaggta acaggaaaat agatatgtaa
     8401 aaagaaagta ggaagataaa atgaaactag cagcgattga tttagacggt accttgcttg
     8461 atcgaaatgg aaagatacca gaagaaaata tccaagcact tcggaaattt gatgaaaatg
     8521 gcggaatcgt aaccgtcgct accggaagga acagcatttc tgctaaagat attttttccg
     8581 aattaggaat cggtggctat ttgatttctt ccaatggttc actgattgct gaaatgaagg
     8641 atggaaaaat ggcgaaagtt ctcagacgtt caaaaataga tagcgataca ctgagaaaag
     8701 ccttcttcta tgctcaagaa gcgaaaatat ccatcattgc tagccgagaa acccaagatg
     8761 atcagctgac attcgatgaa tcaaatctag tcaaagatga cccttattat gaacatttca
     8821 atttacagaa ccattctttt gatcaaattt taaatatgtt ggaagatcct tctcttagct
     8881 atcttaaatt agcattaaca gataagagtg aagaaaaact acagaagcta caaagagatc
     8941 tgcaagcaga tggtatccaa tcagtattct ctgatccgca tttcttagaa atcacgccta
     9001 caggtatcac taaagctcat tcacttgttt ttctggcgga ttaccttgga atcgaatcga
     9061 gtgacatcat ggcttttggc gatcaagaaa acgacattgc tatgttgaat tacagcgggt
     9121 tgagtattgc gatggggaat gcacagcgac aagtaaaaga aattgcaaac caagtaacac
     9181 tgacgaatga agaagctggt gttgcttata ttttaaaaaa cttctattaa aaagaataaa
     9241 atgacgatat gaatgtagga ggaaagaaaa tgttagttat tattgatatg caaaatcata
     9301 ttcttgatcc aaacagcgaa ttttatattc cagagtcaga tcaattggca gaacgtattg
     9361 ctcaaagatt agctaaagca cgccaaaata atgaatatat tctcttcaca cgggatatac
     9421 cagttgaact gaaggacaaa gaagaagaac gagaagattt gcaattgatc cctcttcttt
     9481 ctccacaaga aaatgaacga gtgattaaaa agtattactt tacgattcca cctgaaactt
     9541 tagctgaaat ccaggaaact ttatttgaaa gcaaagaaga acaaaaagag atcgaagtag
     9601 tcgggattga aacaaacttg tgtgtactct ctaatctgat aggcctgcag agtgcttttc
     9661 ctgaagcaga cttttttgtt gatccaatgt tagtctccag tagaaaacac ggagaatccg
     9721 ctttagagct gttgaaagat ttcaacgtct ctatcgtcga atcataaaaa tatctatctt
     9781 catttacgat atttaacaca caataaaaaa agcacgcaga actgttttag tcctacgtgc
     9841 tttttctatc aaagtagatt ggctttttta tgacacagag tgaaacggca ggctcacgac
     9901 ctctttctta aatggtgttc aatcagctga ccctcggtat tagcttaaca aacggcaaaa
     9961 tatgaggaac tatttttccg tttgttaagc taatactcag gtcaaaacgc tgatttcaca
    10021 acctcttcct taaacggtat tcaaaagctt gcatctgcag taataagaac agccaagtaa
    10081 aaacataaga agctgttttt acttggctgt tcttattact tgatgcaggg cagctttttc
    10141 acaacctctt atgtaccttg taggactcga acctacgacc ggacggttat gagccgtctg
    10201 ctctaaccaa ctgagctaaa ggtacatcat aaataaaatt tatttttgtt acttttcaac
    10261 tatagcacga atgacaaact ctcacaagtt tcaaaatata agtaaaataa tattgttatt
    10321 cttatttctt ttacttgtgt cataactatt ttgttacttt aatttttatt tgcttatttt
    10381 agaaaaaatg ttattctttt atttattctc aatgttagga gtgattattt tgccatttat
    10441 tgcgatttta ttcgatttag ttgcggcagc agcttatttt ctccaattaa attatcaaac
    10501 ctcaggaatt ctgtttattg gaatgatttt tcaaggtgtg attacccttt tgcttcttgt
    10561 catgatgctc agctataaag gaaaaagata tgcccgtatt caaacaaaag tatttgttaa
    10621 atatgtttcc attcgctatg ggatcatcat tctttccttt ttagtgaatg cgatcgtcct
    10681 ctttttatat gtattgaatt acttagatat caatccattg attttttctc gatagataag
    10741 acacattcag cggaacagtt cctgcttatc agcagaaaac tgttctgctt tttcattttt
    10801 cattgatcta ttcttctttt attttcctag catttcattt gtgatgtatg caataacatg
    10861 ttatctttta ttatatgaat cgttgaggag tccgttacat gattacgttg gtgagagttt
    10921 tgttttgggt gcctgcggtc gcactggtcg caagtattgt ttatcttatg aattggaaca
    10981 aagaacggtt ttatttagct attttgactc ttccggctat ctactttatg tggaaagttt
    11041 ttaattacaa ttactttgaa cccgatagtg tttttataga agaattatca ggactggttt
    11101 tgtctttatt gatcgtcatt ctttatttga tacgtttaaa taaaaaacat tgagtactat
    11161 tgattgcatg tgtagctttc tatagtcatt ataactattc tactataatg aaaggtgatt
    11221 aaatgagtat cgaagaagct attttactgg atactttacg ttgtcgtctg aatttcttaa
    11281 gaactggccg atctttatct caagtagctg tttctagcgc gttaagtatt cctttattca
    11341 aatatcgtaa gtacgaagaa ggagaagaaa ttccaagcat cattgaatta atctatatcg
    11401 ctgactttta cgatatttcc gttgattacc taattggtcg ctcagaaaca gcaagcaaac
    11461 tgaagtgatt ttttccagcc ttggtgctgg ttttttcatt taacgtctaa ggttaacaat
    11521 ctatatataa aggagacatt tctatgcgat tatggcatga aaaattgatt tcaaagcttc
    11581 ctcgtcaaca gcttttagga caacatcggg aatgctgtgc attaagagga aatggctggg
    11641 gaaagaaaca tgctactgtg gattatgttt ttcactattc tccttataga ttattccaat
    11701 atcatcagct tgtcatggat gaaatggaaa aacgtggtta cagcccagca ccagagtgga
    11761 aaaatcctat gtatcgtggc aaatcatgcg aagcattttt tgatttacct actgtagagc
    11821 gcaacgtgcc gatttatccg gaacacgatg aagtttatct agcagaatgt ttagcaaatt
    11881 tggagcaaaa aggaatttat cttacctaat ataactagaa aagccttcta tctcctctta
    11941 cttcattaaa aagctttaac catcttagat caagctaaaa tgtcgtaagt tttagggtta
    12001 tcttcatcct cacgtgcaaa actaaaagcg tactctagtt ttggttcatc gccaacttcc
    12061 ctagacataa atgaacatct cctgacgtgg tttaaaagta ccgcatcttc gacgtctgta
    12121 tacgatgcac agcacagatt gcttcttttg atctttcttt tgatttttct gttgcttctt
    12181 ctacaccttc ttcgattact acatcgatgc ttacaattgc ttctgcctaa tattcccatg
    12241 catcgtcgat ttaagcctct gtagtcactg tgatcgtttc cacattttaa ctcctctgtc
    12301 gtggcttcag ttgattttgt gatcagttca gttgctaagc cagagtcaga acaacctgcc
    12361 attgctagcg ttaatattga tagtaaaaat agttttttta tcataaggtt ccccccctga
    12421 aaatagacta aaataataac gctttctttt tttgttttca gacgaaa
//
LOCUS       DAZKLD010000089        12446 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.089, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000089 DAZKLD010000000
VERSION     DAZKLD010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12446)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12446
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.089"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            359..922
                     /gene="efp"
                     /locus_tag="ACG8KR_001954"
     CDS             359..922
                     /gene="efp"
                     /locus_tag="ACG8KR_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HFQ0201314.1"
                     /translation="MISASDLRAGMTFVQDGKLIKVLDASHHKPGKGNTVMRMKLKDV
                     RSGATYDTTFRPEEKFEKAHIDTKPVQYLYTMDDTAFFMDLETYEQYEIPVETVEEEM
                     KYILENMEVKIQFFGSEVIGVQLPTTVVLRVVETQPSIKGATVTGSGKPATMETGLVV
                     NVPDFIETDELLEINTAEGSYVKRASK"
     gene            1023..1193
                     /locus_tag="ACG8KR_001955"
     CDS             1023..1193
                     /locus_tag="ACG8KR_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0201315.1"
                     /translation="MKNKKVFDFIYILKDEWQKLEKEKTALIYEEIEMIHKIENIRTI
                     PNYIEAKIFFHH"
     gene            1408..2667
                     /gene="lysA"
                     /locus_tag="ACG8KR_001956"
     CDS             1408..2667
                     /gene="lysA"
                     /locus_tag="ACG8KR_001956"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HFQ0201316.1"
                     /translation="MTELIIGGVTASTLAEKYKTPLYVYDEEKMRQTMLSFKRGFVSK
                     RFDTKVLYASKAFQTIEMLHLVREYGFGLDVVSGGEIYTALQADFPRDTIYFHGNNKT
                     PEELIYALENDLLHIVADNLMEVELLAELSQRYLKKICIMLRLNVGVEAHTHEYIVTA
                     HIDSKFGMSYESKECQQALQLIKESSYLELEGFHAHIGSQIFDVTAWLAEIDKLTNYL
                     TDFDQPLSLNLGGGFGIRYTQNDQPIPVEEALKKLIEYTEEALEKRKLTIRQLLIEPG
                     RSIVGEAGTTLYTIGFIKKTPHKKYYFVDGGMTDNIRPALYQAEYDCDLAAKLSEEKT
                     EKVTIAGKMCESGDVVIKEAYLPKAESGDLLAVYSTGAYGYSMSSNYNRATRPAVVFV
                     NNGQSRLIVRRQAFSDLLRGEVSYDPS"
     gene            2654..3454
                     /gene="dapF"
                     /locus_tag="ACG8KR_001957"
     CDS             2654..3454
                     /gene="dapF"
                     /locus_tag="ACG8KR_001957"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HFQ0201317.1"
                     /translation="MILHKYNGCGNDFILLDYAEDTDYSQLAASLCTKEKYDIDGLIA
                     VKIDPLEMIYYNKDGSRAPMCGNGIRCFARYVNDQNMMNEQEFNVETLAGTRKITVLD
                     PSPFYCSVDMGVPDDSPEKIKANQTYPIKNQEIEINGEKVTITSLFLGTIHTVVFVEN
                     AREEVWRKRGELICHHPLFKEKTNVNFVEVVNEQELIVRTYERGVGWTLACGTGCCAS
                     YVVAKETGKISADQVVVHLEQGDLMISGEDAIQMAGPAVHEWTRILEE"
     gene            3457..4338
                     /gene="dapA"
                     /locus_tag="ACG8KR_001958"
     CDS             3457..4338
                     /gene="dapA"
                     /locus_tag="ACG8KR_001958"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HFQ0201318.1"
                     /translation="MIKGSIVALITPMNEEGSVDYAGLEKLIQFHLDEQTDGLLVLGT
                     TGESSTLTQSEEEQILQLTVKKVAGRVPVIAGAGTNNTKETIEKAKHFASLGADALLV
                     ITPYYNKTSDAGLAAHFTAIAEASPIPLILYNVPSRTGMSIPIHVLVNLAEHPNIIGL
                     KEASGDMAYVMDAARLIGEEFFLYSGNDDLILPVMSVGGSGVISVWANIQPKIVHELV
                     KDTQDGRWQQAKEKQLNALELIHALFSETNPIPVKAAMSLLDLPSGPLRLPLVSLSKE
                     KKKQLAQLLLKERTVEK"
     gene            4335..5051
                     /gene="dapB"
                     /locus_tag="ACG8KR_001959"
     CDS             4335..5051
                     /gene="dapB"
                     /locus_tag="ACG8KR_001959"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HFQ0201319.1"
                     /translation="MKIGLIGSGQMGNRVEEVAQERGDTVLLLRTAPKEATTLAFTEL
                     PELLIDFSHPDNLEKILSYSLSYQLPLVIGTTGYTQSQFQEIKEHAKHVPVMYSANFS
                     FGIMVMNQLIKQAAAMLQGWQIELIEKHHSRKKDAPSGTANMLLQTIQEVQKLQPVYN
                     WQGKKREENQIGVHSIRAGSLPGEHDVLFAATDEIFTIKHESFSNRIFAAGAVEAADW
                     LKDQSPGYYKLEDLLMDKGV"
     gene            5057..5752
                     /gene="dapD"
                     /locus_tag="ACG8KR_001960"
     CDS             5057..5752
                     /gene="dapD"
                     /locus_tag="ACG8KR_001960"
                     /EC_number="2.3.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047200 - tetrahydrodipicolinate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HFQ0201320.1"
                     /translation="MEAQEIIRYIQTASKKTPVKVYLNVTEPIEFKESKVFGEGSSFV
                     VFGDYETIAPVLEAESEKIIEIEIETAARYSAVPLLDIKKINARIEPGAIIRDQVSIG
                     NNAVIMMGAIINIGAVIGENTMIDMGAVLGGRATVGKNCHIGAGAVLAGVIEPASAKP
                     VIVEDGVLVGANAVIVEGVHIGKDAVVAAGAVVLEDVAAETVVGGIPARVLKISDDQT
                     KENTALIAALREL"
     gene            5768..6769
                     /locus_tag="ACG8KR_001961"
     CDS             5768..6769
                     /locus_tag="ACG8KR_001961"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HFQ0201321.1"
                     /translation="MREIDTCIVGATGLVGQTMLKVLEEYDFPVGRLKLLASKRSAGK
                     VCVFKKKKHTVQELTETSFEGYDLALFSAGASVSKEFAPIARSQGLFVIDNSSAWRED
                     SDIPLIVPEVNLEDYTLNRLIANPNCSTIQAVLPLKALQEAFGIQRVNYSTYQAVSGS
                     GQKGIEDLHRTQNDEEPVLYPYNISQTVIPEIDQPLENGYTKEEQKMINETRKILHQP
                     ALPVSATCVRVPIENGHGVSVAVQLQKEFTTEEIRECLSQFPGIQLEDDLAAHKYPTS
                     ILARGTDMVYVGRIRKDTSLENGLLFYTTADNIRKGAAANAVQIAAALMQKIAEVAR"
     gene            6766..8127
                     /locus_tag="ACG8KR_001962"
     CDS             6766..8127
                     /locus_tag="ACG8KR_001962"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HFQ0201322.1"
                     /translation="MSNTLKVAKFGGTSMSHEEQFKKVRTIVLSDPNRQVIVVSALGK
                     RTSDDDKVTDLLYLIYAYLQHHVDWTPLWKRICDRFIQVRDVLALQLPIEDELARIQK
                     QLEDEKITEDYLVSRGEYLTAQLMAEYLGYQFIDAKDLIFFDYDGKIDQEKTAQEITK
                     AFKRYPKMVVPGFYGANPAGKEKLLGRGGSDITGAILAKMLQAESYENWTDVSGIMMA
                     DPRIIDHPKIIEEISFRELREMAYMGANVLHEAAVFPVQEADIPIQIKNTNDPFAQGT
                     KISNHEIEKENGLTGIAGRKDFLSITLFKRHMSDEIGFIWKAMSIFAKHEISIEHIPS
                     GIDNIGVIVSAEAIADKLFLITKELKETLGVEEIVVIEDLALISVVGGPHKELIGLSG
                     KVLSILNKLEIRTSILSQGAQELNLIIGVPNNQYETVVKGIYEGMVNTDAERISNGTP
                     SNS"
     gene            8087..9238
                     /locus_tag="ACG8KR_001963"
     CDS             8087..9238
                     /locus_tag="ACG8KR_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFQ0201323.1"
                     /translation="MLKEYRMALHQIPEIGFHEFKTKKYLYEQVKNSEAVIHEIGETG
                     LLLYFDNQQEETIAFRTDIDALPVKEETNLSFASKHEGYMHACGHDGHMAMLLGLTDF
                     ITKHIEDLIYNVVLIYQPSEEISGGAQSVIQSGLLDKYEVQAIFGFHLWPGLAKGEVF
                     SRPGPLMAQSSETDIIIKGQSAHIASSEKGIDSLEAAVRFLKNVYDFDRQLPAEWNHL
                     MKFGQIQGGTIRNVLAGEVIVSGSIRSYSSDMQNYLKRGLDHLAEQFHSTATISFRYN
                     DGYPAVINHRGLYAALLDSNKLHELDAPVLQAEDFGIYTQQYPCVFFFLGVGDTPALH
                     DPTFDFDMDVLENGVEWYKTILFTENLLVKIDSSSKEERVNNDEFIQSI"
     gene            9213..10382
                     /locus_tag="ACG8KR_001964"
     CDS             9213..10382
                     /locus_tag="ACG8KR_001964"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFQ0201324.1"
                     /translation="MMNLSNRFSPRLSRIEVSKIRQFDQQISSIPDVIKLTLGEPDFP
                     TPEHVKQAGIAAIEEDFSHYTGMRGLEELREAACIFQQQRYGLTYDPQTEVLTTVGAT
                     EAIASALLSVLEEGDKVLIPAPAYSGYQPLVELAGAELIPIDTSDTGFVCQPEQFERA
                     FEQYGSAVKAVILNYPNNPTGTTLSAKQLKAIAEVLKKYPVFVISDEVYAELTYSGTH
                     MSIATYLPEQTIVISGLSKSHAMTGWRVGFIFAQKALIDELIKVHQYLVTAATTMSQK
                     AAAEALINGVKDSENMKEQYQTRRDYLVQQLAPLGFEVTQPNGAFYLFCKLPAAIQTD
                     SWQFCVELAEQAKVACIPGIAFGPEGEGYIRISYASSMEKLHEACKRIKDFLTEQ"
     gene            10557..11936
                     /locus_tag="ACG8KR_001965"
     CDS             10557..11936
                     /locus_tag="ACG8KR_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFQ0201325.1"
                     /translation="MEKIKVVIVGASHGGHQSILELLTRYGENVDITLFEAGDYISFM
                     SCGMELYLEDKVTDVNDVRNFRPENFPQSNVHILNNHEVKTIDADKKTVTVTSASDNE
                     TKEYPYDKLILSSGVKPNNLPVPGADLENVYLMRGYNWATKIKEKLNDPAIKKIAVVG
                     AGYIGIEAAEASRKAGKEVVLLDVIDRPLGTYLDAEMTDILEQHLNENGIEVLTNAKI
                     EAFTGNGKVEAIKTSEKEIPADLVIQAAGVKANTEWLKGIVDLDERGWIQTNEYLQTN
                     LPDVYAVGDATLAYSIPARKKMPIALATVARREARYVVKHLFEEVPNKPFGGVVGSSA
                     LSVFDYHFAASGLNSFTAAKAGVDVRVSYYEDTLRPKYVPEEQGNPKVSIQLFSDPMT
                     HQLLGGAVLSTYDVTGQGNVLALAIQNKLTLEELAEADFFFQPGFDRQWSMLNLAAQQ
                     ALGEEPFVE"
     gene            12217..12446
                     /locus_tag="ACG8KR_001966"
                     /pseudo
     CDS             12217..12446
                     /locus_tag="ACG8KR_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         4201 a         2138 c         2734 g         3373 t
ORIGIN      
        1 gaaaaattga gtagataaaa agataagtta cctggtactc attctatgaa gggcaattaa
       61 tagacatgac aaatagtatg ctgcttttat gcaccataca acagcaacga ttcaattgtt
      121 tttggttgct gactgtcatg tgtatccctc caagtaaaat ttttattaac gatagcatga
      181 aaaaaaataa aagtcaaagg cttgaaagaa aaaaatattt cgatagcatc gtttacccgt
      241 gtttattttt ctgacaaata ggcatgagac cttaaaagat agagaatact ctagttttct
      301 ggccggttgt ctgatacaat aaagttgatt atgataaaga aaagaaggag caaaaataat
      361 gatctcagca agtgatttaa gagccggtat gactttcgta caagacggca aactgattaa
      421 agttctggat gcaagccacc ataaacctgg taaaggaaat acagttatgc gtatgaaact
      481 gaaagatgtc cgttcaggtg caacatacga cacaacgttc agaccagaag aaaaatttga
      541 aaaagcacat atcgacacaa aacctgtaca atatttgtac acgatggatg atacagcttt
      601 cttcatggat ttggaaacat atgagcaata tgaaattcct gtagaaacag tagaagaaga
      661 aatgaaatat atcctagaaa acatggaagt aaaaatccaa tttttcggta gcgaagtcat
      721 cggcgttcaa ttaccaacaa ctgtcgtatt acgtgttgtg gaaacacaac cttccatcaa
      781 aggtgcaaca gtcactggtt caggtaagcc agcgacaatg gaaactggct tagtagtcaa
      841 tgttcctgac ttcatcgaaa cagatgaatt gttggaaatc aatacagcag aaggttctta
      901 tgtaaaacgt gcgtcaaaat aattcgctaa cggattgata agaatttgaa aacagagctt
      961 gacctcttga aaggcagatc atcggatctg cctttcaaga ggttttttgt ggaatcagca
     1021 aattgaaaaa taaaaaagta tttgatttta tttatatttt gaaagatgaa tggcaaaagc
     1081 tagagaaaga aaaaactgca cttatttatg aagaaattga aatgattcat aaaattgaaa
     1141 atatccgaac tattccgaac tatattgaag cgaaaatatt ttttcatcac taaatagtgt
     1201 tgaatttatg tttttatgta taaatagctt ctaatagttc gggtattttg tttttataac
     1261 aatttttatt cttattttta aaaaaatatt tcgaaatata ctagacaatt cagaaaattt
     1321 tgtttaatat tattccaata aaattcaaat ctttatcaaa tgaaattcca ataaaaacgg
     1381 aaaaaaagtg aaaagaggaa tgaaaaaatg acagaattga taattggcgg agtcacagca
     1441 agcacattgg cagaaaagta taagacacct ctgtatgtgt atgatgaaga aaagatgaga
     1501 caaacgatgc tgtcattcaa gcgaggcttt gtatcgaaac gttttgatac aaaagtgctt
     1561 tacgcctcta aagcattcca aacgattgag atgctgcatt tagttcgtga atatggattc
     1621 gggctagatg ttgtcagtgg tggggaaatc tataccgctt tgcaagctga ttttcctaga
     1681 gatactatct acttccatgg taacaacaag acgccagagg aactgatcta tgcacttgaa
     1741 aatgatttgt tgcatattgt tgccgataat ttgatggaag ttgaattatt agcagaatta
     1801 agccaacgct atttaaaaaa aatctgtatc atgcttcggt tgaatgtcgg agtagaagca
     1861 catactcatg aatatatcgt cactgcgcat attgattcaa aattcggcat gagctatgaa
     1921 agtaaagagt gccaacaagc ccttcagttg ataaaagaaa gcagctatct agagttagaa
     1981 ggatttcatg ctcatatcgg gtcacaaatt tttgatgtta ctgcttggtt agctgaaatc
     2041 gacaaattga cgaattactt aaccgatttt gaccaacctc tttccttgaa tcttggcgga
     2101 gggtttggga ttcgttatac acaaaatgat cagccgatcc cagtcgaaga ggcactaaaa
     2161 aaactgattg aatatacgga agaagctttg gaaaaacgaa aactgaccat tcgtcaatta
     2221 ctgatcgaac cgggtagaag tattgttgga gaagctggta caacactata cacgattggt
     2281 ttcatcaaaa agacaccaca taaaaaatat tactttgtag atggcgggat gacagataat
     2341 atccgtccag ctctgtatca ggcagaatac gattgtgatt tagcagcaaa actgtcggaa
     2401 gaaaaaacag agaaagtgac gattgcagga aaaatgtgtg aatcaggcga tgtggtaatt
     2461 aaagaagctt atttgccgaa agcagaatcg ggtgatcttt tagctgttta ttccacggga
     2521 gcttatggat actcgatgag cagcaactac aatcgagcga cacgaccagc agttgttttt
     2581 gtcaataatg gccaatcaag actgattgtt cgcagacaag cattttctga tctactaagg
     2641 ggggaagttt cctatgatcc ttcataaata taatggctgc ggaaatgatt ttatcttatt
     2701 agattacgca gaagacacag attatagtca attagccgct tctttatgca caaaagaaaa
     2761 atatgatata gacggattga tcgccgtaaa aatcgatccg ttggaaatga tctattacaa
     2821 caaagatggc agccgcgcgc cgatgtgcgg aaatggcatt cgctgttttg ctcgatacgt
     2881 aaatgatcag aacatgatga atgaacagga attcaacgta gagacattag caggcacaag
     2941 aaaaattacg gtgttggatc cctcaccttt ttattgcagc gtagatatgg gagtgccaga
     3001 tgattctccg gaaaaaatca aagctaatca aacttatcca ataaaaaacc aggaaataga
     3061 gatcaatggt gaaaaagtaa cgatcactag tttattttta ggaacgattc ataccgttgt
     3121 ttttgttgaa aatgcgcgtg aggaagtctg gagaaaaaga ggtgaactga tctgtcatca
     3181 tcctttgttc aaagaaaaaa ccaatgtcaa ctttgtagaa gtagtcaatg aacaagaatt
     3241 gatcgtccgt acctatgaaa gaggagtagg ttggacactt gcttgcggta caggttgctg
     3301 tgcctcttat gtggttgcta aggaaactgg gaaaatttca gcagatcagg tagttgttca
     3361 cttggaacaa ggagacttga tgatctctgg tgaggatgcg atacaaatgg ctggcccagc
     3421 agttcatgaa tggacaagaa tattggagga atgaagatga taaaaggttc gatcgtggcg
     3481 ctgatcacgc cgatgaatga agagggatca gtagattatg ctggtttgga aaaattgatc
     3541 caattccatt tagatgaaca aacagatgga ttattagttt taggtacaac aggagaatct
     3601 tctacgctca cacaatctga ggaggagcag atccttcaac tgacagtaaa gaaagtggct
     3661 ggccgtgtgc cggtgattgc aggagctgga acgaataata cgaaagaaac gattgaaaaa
     3721 gcgaagcatt ttgcttcttt gggggcagac gcacttttgg tcatcacccc ttactacaac
     3781 aaaacaagtg atgcaggact agctgctcat tttacagcaa ttgcagaagc ctcaccgatt
     3841 ccgcttattt tgtataatgt tccttctaga acaggcatgt cgattccaat ccatgtatta
     3901 gtaaatttag cagaacatcc taatatcatt ggtttgaaag aagcctcagg agatatggct
     3961 tatgtgatgg atgccgctcg gttgattgga gaagaattct tcctttacag tggaaatgat
     4021 gatttgatcc taccagtaat gagtgtagga ggaagcggtg taataagtgt atgggcgaat
     4081 atccaaccta aaatagtcca tgaattagta aaagatacgc aggacggaag atggcagcaa
     4141 gcaaaagaaa agcaattaaa tgcacttgaa ctgatccatg ccttgttcag tgagaccaat
     4201 cctattcctg tcaaagcagc catgtcatta ttagacttac cttccggacc tctacgcctg
     4261 cctttagttt ctctttcaaa agagaaaaag aaacaattag ctcagctttt gttaaaagaa
     4321 aggacggttg aaaaatgaaa atcggactga ttggcagtgg acagatggga aatagagtcg
     4381 aagaagttgc acaagaaaga ggagatacgg tccttctttt acggacggca cctaaagaag
     4441 caactacgct tgcttttacg gaactgccag aactgcttat tgatttttct catccagata
     4501 atttagagaa aatcctttcg tatagcttat catatcaact tccgttagtc attggcacga
     4561 caggttatac acaatcacag ttccaagaaa taaaagaaca tgccaaacat gtacctgtga
     4621 tgtatagtgc taatttttcg ttcggcatca tggtgatgaa tcaactaatc aagcaagcag
     4681 ctgcaatgtt gcaaggctgg cagatcgaac tgattgaaaa acatcatagt cgtaaaaaag
     4741 atgcaccatc tggtactgca aatatgctgc ttcaaacaat ccaagaagtg caaaagctgc
     4801 agcccgtata caactggcaa gggaaaaaaa gagaagaaaa ccaaattggt gtacacagta
     4861 ttcgtgcagg ttctttacct ggtgaacatg atgtattgtt tgctgctact gatgaaatat
     4921 ttacgatcaa acatgaatct ttttctaatc gaatctttgc tgcaggcgca gtagaagcag
     4981 cggactggtt gaaagatcag tcaccaggtt attacaaatt agaagattta ttaatggaca
     5041 aaggggtgta aaaaacgtgg aagctcaaga aatcatccgc tacattcaaa cggcatctaa
     5101 aaagacgcct gtgaaagtct atctgaatgt aaccgagcca atcgaattta aagaaagtaa
     5161 agtttttggt gaaggaagca gcttcgttgt ttttggtgac tatgagacga ttgctccggt
     5221 tttagaagct gaatcagaaa aaatcattga aatcgaaatc gaaactgctg caagatacag
     5281 cgcagttcct ttgctagata ttaaaaaaat taatgctcgt atcgaaccag gtgctattat
     5341 cagagatcaa gtgtcaatcg gcaacaatgc cgtgatcatg atgggagcga ttatcaatat
     5401 tggtgcagta atcggtgaaa acacgatgat tgatatgggt gctgtcttag gtggacgagc
     5461 gacagtgggc aaaaactgcc atattggtgc aggagctgtc ttagcaggtg tgatcgaacc
     5521 agcatcggcc aaaccggtta tcgtagaaga cggcgtactg gtaggtgcga atgcagtgat
     5581 tgttgaaggt gtccatatcg gaaaagatgc agtcgtggca gcaggagcag tggttttaga
     5641 agatgtggca gcagaaactg ttgtcggtgg aatacctgca cgtgtattaa aaataagtga
     5701 tgatcagaca aaagaaaata cagcattgat tgctgcgtta cgcgaattat agaggagatg
     5761 gagaaaaatg cgcgaaatag atacctgtat tgtgggggca accggattag tcggccaaac
     5821 gatgctcaaa gtgttagaag aatatgattt tccagtagga cgattgaaat tattagcttc
     5881 aaaacgttca gccggtaaag tttgtgtatt caagaaaaag aaacacactg tacaggaatt
     5941 gacagaaact tcatttgaag gatatgattt agcgctattt tctgcaggtg cttctgtatc
     6001 caaagagttt gcgccaatcg cacgaagtca gggactattt gtcatagata attcctcagc
     6061 atggcgggaa gattcggata ttccattaat cgttccagag gtcaatctgg aagactatac
     6121 attgaatcgg ctgattgcaa atccaaattg ttcaaccatt caagcggtac ttccgcttaa
     6181 agctttacaa gaagcctttg gtatccagcg agtcaattat tcgacctatc aagcagtttc
     6241 tggttccggg caaaaaggca ttgaagatct tcatcgtacg caaaatgacg aggaaccagt
     6301 tttgtatcct tataatatta gccaaacggt gattccggaa atcgatcagc ctttggaaaa
     6361 tgggtataca aaagaagaac agaaaatgat aaatgagacc cgtaagattc tgcatcagcc
     6421 tgctttgcca gtatcagcaa cctgcgtacg tgtgccaatc gaaaatggac atggagtctc
     6481 tgtggcagtt caactgcaaa aagagtttac gacggaagaa attagagagt gtctcagcca
     6541 gtttccaggg attcaattag aagatgatct agcagcacat aaatatccga cgagcattct
     6601 tgctagagga acagatatgg tttatgttgg gaggatcaga aaagacactt ctttagagaa
     6661 cggattgttg ttctatacga ctgccgataa catacgcaaa ggagccgctg cgaatgccgt
     6721 acagattgct gcagctttaa tgcagaaaat agcggaggtg gcaagatgag taatacgctg
     6781 aaagtagcga aattcggcgg aacatcgatg tctcatgaag agcaattcaa aaaagtccgt
     6841 acaattgttc tttcagatcc caatcgccaa gtgatcgttg tttctgcttt aggcaaacgt
     6901 acgtcagatg atgacaaagt aacagactta ctttatttga tttatgctta ccttcagcat
     6961 catgttgatt ggacacccct ttggaaaagg atttgtgatc gtttcattca agtaagagat
     7021 gttcttgccc tgcagttacc tattgaagat gaactagcac gcatacagaa acaactggaa
     7081 gacgaaaaga taactgaaga ttatttagta agtagaggtg aatatctgac tgctcaactg
     7141 atggctgagt atcttggtta ccagtttatt gacgcaaaag acctgatttt ctttgactat
     7201 gatgggaaaa tcgatcagga aaaaacagca caggaaatca caaaagcatt taaaagatat
     7261 ccgaaaatgg ttgttccagg gttttacggg gcgaaccctg caggaaaaga aaaattattg
     7321 ggccgtgggg gaagcgacat taccggcgcg atactggcga agatgttgca agcggaaagt
     7381 tatgagaatt ggacggatgt ttcaggaatc atgatggctg atccgcgaat catcgatcat
     7441 cctaaaatca tcgaggaaat ctcttttaga gaactgagag agatggctta tatgggggct
     7501 aacgttcttc atgaagcagc tgttttccca gttcaagagg ctgatatccc catccaaata
     7561 aaaaatacca atgatccttt cgctcaaggg acaaaaatat cgaatcacga aatagaaaag
     7621 gaaaatggac tgacaggaat agctggacgc aaggattttc tttccatcac tttattcaaa
     7681 cgtcatatgt ccgatgaaat cgggtttatc tggaaagcaa tgagcatttt tgctaaacat
     7741 gagatatcaa tcgaacatat tccttcaggg atcgacaata tcggagtgat cgtctcagca
     7801 gaagcgattg ccgataagtt atttctcatc acgaaagaat taaaagaaac acttggcgtg
     7861 gaagaaatcg tcgtgatcga agacttagct ttgatctctg tcgttggggg tccgcataaa
     7921 gaattgattg gattatctgg gaaagtgtta tcgatattga acaaattaga aattcgcaca
     7981 tcgatcttat cacaaggagc acaggagttg aatctgatca tcggtgtacc gaataatcaa
     8041 tatgaaacag tagtgaaggg tatctatgaa gggatggtga atacggatgc tgaaagaata
     8101 tcgaatggca ctccatcaaa ttcctgaaat cgggtttcat gaattcaaga caaaaaaata
     8161 tttatatgaa caagtaaaaa acagcgaggc cgttatccat gaaatcggcg aaacaggact
     8221 actcctttat tttgataatc agcaagaaga aacgattgct tttcgaacag atatcgatgc
     8281 actgccagtc aaagaagaaa cgaatctctc ttttgcttct aagcatgaag gttatatgca
     8341 tgcatgtggt catgacggac atatggcgat gcttttaggg ttgactgact ttataactaa
     8401 acacattgag gatcttattt ataatgtagt actgatctat cagccatcag aagaaatttc
     8461 cggtggcgca caaagcgtta ttcagtcagg attgttggat aaatatgaag tacaagcaat
     8521 atttggcttt catctatggc ctggactagc taagggagaa gttttctctc gaccaggacc
     8581 tttgatggca caaagcagtg aaacagacat tatcattaaa ggccagtctg cacatattgc
     8641 ttctagtgaa aaaggaatcg atagtctgga ggcagctgta cgatttttga agaatgttta
     8701 tgattttgac cggcaattac cggcagagtg gaatcatcta atgaaatttg gccaaatcca
     8761 aggtggaacg atccgaaatg ttttagctgg agaagttatt gtatccggaa gtatccgttc
     8821 ttacagttca gatatgcaaa attatttgaa gaggggatta gaccatttag cagaacagtt
     8881 tcactctaca gctactattt cttttcgcta taatgacggc tatccagctg taatcaacca
     8941 tagaggctta tatgcagcac tgttagattc aaacaaacta catgaattag atgctccagt
     9001 tcttcaagcc gaagattttg gcatatatac gcaacaatat ccatgtgtat ttttcttttt
     9061 aggtgtcggt gatacccctg ctttacatga tccgacgttc gattttgata tggatgtttt
     9121 agaaaatggt gtagaatggt acaaaacgat tctttttaca gaaaatcttc ttgtaaaaat
     9181 cgatagcagt tcaaaagagg agcgagtaaa taatgatgaa tttatccaat cgatttagtc
     9241 ctaggctttc tagaatagaa gtatcaaaaa tacgacaatt cgatcagcaa atatcgtcga
     9301 taccagatgt gatcaaactc accctgggag aaccggattt tccaactcct gaacatgtca
     9361 agcaagcagg aatagcagcg attgaagaag acttttctca ctataccgga atgagaggat
     9421 tagaggaact gcgggaagcg gcttgtatat ttcagcaaca gcgttatgga ttaacttatg
     9481 atccgcaaac ggaagttttg acgacagttg gtgcaacgga agcgattgcc tctgcgttgt
     9541 tatctgtttt ggaagaagga gacaaggttc ttattccagc gcctgcttat tcaggatatc
     9601 agccattagt ggaactagct ggtgctgaat tgatcccgat agatacttct gacacagggt
     9661 ttgtctgtca gccggaacaa tttgaacgag catttgaaca atatggatca gctgtcaaag
     9721 cagtcatttt aaactatcct aacaatccta ctgggacgac attgtctgca aaacaattga
     9781 aagcaatcgc tgaggtttta aaaaaatatc cggtatttgt tataagtgat gaagtatatg
     9841 ctgaattgac ttatagtgga acgcatatgt ccatagctac ttatcttcca gaacaaacaa
     9901 tcgtgataag cgggttatcc aagtcacatg cgatgactgg ttggcgagtg ggctttattt
     9961 ttgctcaaaa agcccttatt gacgaactaa ttaaagtcca tcaatatctg gtgactgccg
    10021 ctacaacgat gtcgcaaaaa gctgcggctg aagcgctaat aaatggggtg aaagatagcg
    10081 agaatatgaa agagcaatat caaacacgaa gagactatct ggtccagcaa ttggcgcctt
    10141 taggatttga agtgacccag ccaaacggtg cattttactt gttttgcaaa ctcccagctg
    10201 ccattcaaac tgactcttgg cagttttgtg tagaattagc ggaacaggca aaagttgcgt
    10261 gtatcccagg aattgcattt ggcccagaag gagaaggtta tatccggatt tcttatgcta
    10321 gcagcatgga aaaattacat gaggcctgca aacggatcaa agacttttta acagaacagt
    10381 gattcataga ttttatcaaa aaaacaatcg ttaattttgc ggtatattct gcaaggagac
    10441 gattgttttt taaatttagt tcaaatgaac gcgtttcttt tgattcataa ctaatgtaat
    10501 gttaagttta aattgtttag aatgatttta aaagaatatt caaggaggtt tcatcaatgg
    10561 aaaaaatcaa agttgtcatt gtaggtgcat ctcatggagg gcatcaatcg atcttggaac
    10621 tattgacacg atacggtgaa aatgtagata tcacgctatt tgaagcagga gactatattt
    10681 ccttcatgtc atgtggtatg gagctgtatt tagaagacaa ggtaacagat gtaaatgatg
    10741 tacgtaattt cagaccagaa aactttccgc aatccaatgt tcatatttta aataatcacg
    10801 aagtaaaaac gatcgatgcc gataaaaaaa cagtaacggt tactagcgca tcagataacg
    10861 aaacaaaaga atatccttat gacaaattga ttttgagttc tggtgtcaaa ccaaacaatc
    10921 tgccagtacc aggagcagat ctagaaaatg tctacttgat gcgtggatac aattgggcaa
    10981 ccaaaatcaa agaaaaatta aatgatccgg ctattaaaaa aatcgctgtc gtaggtgcgg
    11041 gttacattgg gatcgaagca gcagaagcaa gccgaaaagc tgggaaagaa gtcgtattgc
    11101 ttgatgtgat cgatagacct ttaggcactt atttagatgc tgagatgaca gatattttag
    11161 aacaacacct aaatgaaaac ggtattgaag ttttgacaaa tgcaaaaatc gaagctttta
    11221 ctggtaacgg aaaagttgag gcaatcaaaa caagtgaaaa agaaatccca gcagatctag
    11281 tcattcaagc tgccggtgtg aaggcaaata cagaatggtt aaaagggatc gtcgacttag
    11341 atgaacgtgg ctggatccaa acaaatgaat acttgcaaac gaatcttcca gatgtgtatg
    11401 cagtaggaga tgctacactt gcttattcta tcccagcacg caaaaaaatg ccgatcgctt
    11461 tagcgactgt tgcacgtaga gaagcacgtt atgtagtaaa acatcttttt gaagaagtac
    11521 caaacaaacc gttcggcgga gtcgtaggtt cctctgcatt aagtgtcttt gactatcatt
    11581 ttgcagcaag cggattgaat agctttacag cagcaaaagc aggagtagat gtacgagtgt
    11641 cttattatga agatacgtta cgaccaaaat atgtaccaga agaacaagga aatccaaaag
    11701 tgtccatcca attattctcg gatccaatga cccatcaatt gctaggagga gcagtattat
    11761 ctacctatga tgttacaggt caaggaaatg tattggcact agcgatccaa aacaaactga
    11821 ctttagaaga actagcagaa gctgacttct tcttccagcc aggctttgac cgacaatgga
    11881 gcatgttgaa tctagccgct caacaagcgt taggagaaga accatttgta gaataagaaa
    11941 acgaaatatt tgaaaagagg ttgtgaaaaa gctgccttgc atcaagtaat aagaacagcc
    12001 aagtaaaaac agcttcttat gtttttaact tggctggact tattaccgca gatgcaagct
    12061 tttgaacacc gtttattttc aaaaaaggga ctgtgacacg atttgtgtac cgacccccaa
    12121 aagttagacc aaaaaatcta acttttggag gtcggtattt tttatggtta aatatacttt
    12181 tgaacttaaa ttaaagatcg ttcatgatta tttggatggc aaaggcgatt ggaccgtaaa
    12241 aatccagatc aagagattga agcggaaatg cttaaaattc gggagaagca caaggattat
    12301 ggctgtttac ggatgaccaa tgaactaaga aaccgcggtt tctctatcaa taaaaagaaa
    12361 gttcagcgct tgatcaagaa gctaggaata aaagtaacgg catacacgcg taaatcacgt
    12421 cgctacaatt cttatcgagg aaaagt
//
LOCUS       DAZKLD010000090        12421 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.090, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000090 DAZKLD010000000
VERSION     DAZKLD010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12421
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.090"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(477..1064)
                     /locus_tag="ACG8KR_001967"
     CDS             complement(477..1064)
                     /locus_tag="ACG8KR_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0201326.1"
                     /translation="MDFKRSYPKLADYLSNRVDFSRKEQQQSHWHWFTLILFGLMTVA
                     TLSQKLILLTLFIAIVALIKGPVMILWGMLYSFLVGLFPPIGILLSAIFFLLNIGTFT
                     KNWRMTLVGIYFYFYPFGVMALSEVMHWDNHWFIAGSLLLGLILLHVMLTKLYQHYGI
                     GRTIFWYVFSIPFALLTALLPSRLKTKIKGYHKIK"
     gene            1239..2561
                     /locus_tag="ACG8KR_001968"
     CDS             1239..2561
                     /locus_tag="ACG8KR_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFQ0201327.1"
                     /translation="MKGKLKREINLFGALATVMGTVIGAGVFFKTAAVTASTQSTSLT
                     LLAWLLGGFLTICAGLTVAELATAIPETGGAVKYIEAAYGKLPSFLLGWAQSLIYFPA
                     NIAALSIIFATQLTNLLQLSTDYLLLIAVITAVSVTGLNLLGTKVGASVQSVTLIVKL
                     IPIAVIVIWGLLTPGQGTVQLFPIEAGKDVTFVEGLSSALLATLFAYDGWLGVGAMAG
                     EMKRPEKDLPKAIILGLSFVTVVYLLINFVFLKTLPIDHLAGNLNAASEASDVIFGGI
                     GGKLVTIGILISVYGALNGYTLTGIRVPYAMALEDELPFSKQLTKLSKKFTVPYVPAV
                     FQLAVACIMMSLGSFDFLTDMLIFVMWLFSLLIFIGVFILRKKAPELPRPYKVPLYPI
                     VPIIAILGAIFILGMTMMTQTKLALIGIGVTLIGIPVYYQKKKKRSEE"
     gene            2716..3492
                     /locus_tag="ACG8KR_001969"
     CDS             2716..3492
                     /locus_tag="ACG8KR_001969"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HFQ0201328.1"
                     /translation="MIFDSHTHLNAEQFNEDIPETIQRAQEMGVTKMAVVGFDTPTIE
                     KSLQLSHDYPNIYSIIGWHPTEAGSYTKEIENKLQEQLTLPKVVALGEIGLDYHWMED
                     PKEVQDKVFRRQIAIAKEMNLPISIHTREALEDTYQILKEEDVRDIGGIMHSFSGDYE
                     WAKRFLDLGMHISFSGVVTFKKALDVQEAAANVPIERLLVETDAPYLAPVPFRGKRNE
                     PGYTRYTVEKIAELRNLPTEEVAAQTWTNAHRLFGLSEHG"
     gene            3485..4054
                     /gene="rnmV"
                     /locus_tag="ACG8KR_001970"
     CDS             3485..4054
                     /gene="rnmV"
                     /locus_tag="ACG8KR_001970"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043822 - ribonuclease M5 activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HFQ0201329.1"
                     /translation="MDKMKIQEIIVVEGKDDTRRLREVVDADTIETIGSAINEEILMQ
                     IEHAQETRGVIVFTDPDFSGEKIRKIIMEAVPQAKHAFLPRTQAKPKKKGSLGVEHAS
                     DAAILEALKKVVTPDISQTAVSEITRQDLLDYGLIAGAQAKEKREKLGDELRIGYTNG
                     KQLEKRLKMFRITPKELAQAMKIVEEQHE"
     gene            4047..4931
                     /gene="rsmA"
                     /locus_tag="ACG8KR_001971"
     CDS             4047..4931
                     /gene="rsmA"
                     /locus_tag="ACG8KR_001971"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HFQ0201330.1"
                     /translation="MSEYREIATPSRTKEILLKHGFSFKKSLGQNFLTEPNILRKIVE
                     TAQIDEHTNVIEVGPGIGALTEQLAKHAKQVVAFEIDDRLIPVLADTMQPYDNVTIVH
                     QDILKTDLSTAVRETFHEELPLKVVANLPYYITTPIMMHFLESDLVVDELVVMMQKEV
                     ADRISAEPGTKAYGSLSIAVQYYMEASLAFIVPKTVFVPQPNVDSAILKLTRRDIPAV
                     EVTDEKAFFRLTKAAFQQRRKTLWNNLQHSYGKDDQTKAWLAKSLETAGIDPKRRGET
                     LSLQEFAALSNAMSENMQ"
     gene            5382..6011
                     /locus_tag="ACG8KR_001972"
     CDS             5382..6011
                     /locus_tag="ACG8KR_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0201331.1"
                     /translation="MRKNSVRKLTISALLIGMGVIIPMVMPKIMIPPASFTLASHVPL
                     FIAMFFTPGVAVAVALGTTFGFFLTTPVIIALRALSHLVFALIGAWYLQKHPSIVLKN
                     GQFTFANWRFQGFNFVIGVIHSLAEMLVVSIFYFIGNMPETYYSQGYFYTVFILMGIG
                     GLIHSLVDYNIAYFIAGTLSKYFDIPVFTAAKKENRKESIQGYAKKTTY"
     gene            complement(6135..7244)
                     /locus_tag="ACG8KR_001973"
     CDS             complement(6135..7244)
                     /locus_tag="ACG8KR_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0201332.1"
                     /translation="MVEMALKNVYKKYDNAENYSVTDFNLSIKDREFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELNIGGKVMNDVAPKDRDIAMVFQNYALYPHMTVFDNMAFGL
                     KLRKYDKADIKKRVDNAAEILGLTEYLKRKPAALSGGQRQRVALGRAIVRDAKVFLMD
                     EPLSNLDAKLRVAMRAEIAKLHQRLETTTIYVTHDQTEAMTMADRIVIMKDGFIQQIG
                     TPKEVYDTPVNMFVAGFIGSPAMNFFNVTLKNGVITDGHGLKLRIPEGKNKVLVEKGY
                     EGKEVIFGIRPEDIHSEQVALEASPEAVVKSEVVVSELLGAETMLYTKTGSTEFISKV
                     DARDFHKPGEIVDLAFNISKGHFFDKETEQVIKLP"
     gene            7453..7875
                     /gene="mgsA"
                     /locus_tag="ACG8KR_001974"
     CDS             7453..7875
                     /gene="mgsA"
                     /locus_tag="ACG8KR_001974"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HFQ0201333.1"
                     /translation="MKIALIAHDRKKPMMVKLATAYKTILEKHELFATGTTGQRITEA
                     TGLPIHRFKSGPLGGDQQIGAMISEDQLDLVIFLRDPLAAQPHEPDVTALIRLSDVYE
                     IPLATNIGTAEVLLRGLEAGFLDFRRIVHEIDKHPLSF"
     regulatory      8062..8160
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01831"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="THF riboswitches regulate genes encoding folate
                     transporters and folate metabolism; They are most commonly
                     found in Clostridiales and Lactobacillales; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="tetrahydrofolate"
                     /db_xref="RFAM:RF01831"
     gene            8198..8734
                     /locus_tag="ACG8KR_001975"
     CDS             8198..8734
                     /locus_tag="ACG8KR_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HFQ0201334.1"
                     /translation="MKKHRLDARMIAIMGLLIALMVTLSRLVAIETPFIKISVTFIPQ
                     VIMGILFGPFWSGIGAVLADLVGMALFSKSAFFIGFTLNAFIEGAIYGFFFYRKEITW
                     KNAILATLSVTLIINLFLTPLWLALMYHVPLFSWVVWAPRLLKTVIWLPIQSIAIYYV
                     GRSIPYKKILRSLAIHAK"
     gene            complement(8774..10537)
                     /locus_tag="ACG8KR_001976"
     CDS             complement(8774..10537)
                     /locus_tag="ACG8KR_001976"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0201335.1"
                     /translation="MKVKKLSLQTLRRFIPYLTAYAKDIWLAVILGIINGIATVVMTL
                     QIGQSIDQMIGEGQVRFNRLFHLLILFGGIVLINVVSQWLIQVLSNRLAYLSVAKLRK
                     DAFAHLNRLPLRYYDQNSHGNIVSRFTNDMDNISIAISAAFNQLFSGVAIIILSFIFM
                     MRLSPLLTLVVLCSTPIIFLVSWIVARASQTNFDRQQRIIGDISGFVTERIGNQKIVK
                     AFQQEEASQQQFELLNADLYEKGQRAQFSSSLTNPSSRFVDHLAYLSIGLVGGLLALK
                     SGSSITVGVISSFTIYSSQFSKPFIELSGITTQLQTAFSGLERAFDLIDQPEEKPDLP
                     DAYALEPKNIKGKVSFDHVSFSYDPKQRLIEDFNFTAYPGQTIAIVGKTGAGKSTLVN
                     LLMRFYDTTKGTITVDDHDIRSIQRDTLRKSFGMVLQDTWLFDSTLRENLRFGNPEAS
                     DAEINDALKASYMYEFVERLPEKLDTKIGGQGLKISDGQRQLLTIARTMISNPPMLIL
                     DEATSSVDTLTEKKIQQAFLAMMEGKTSFVIAHRLSTIKNADQILVMDQGAIVEMGTH
                     DELLQKDGYYRRLYQAQFTAQ"
     gene            complement(10540..12255)
                     /locus_tag="ACG8KR_001977"
     CDS             complement(10540..12255)
                     /locus_tag="ACG8KR_001977"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0201336.1"
                     /translation="MIQLLRYAKDYRKQIILGPFFKFLEAVFELVLPLMMASLIDNGL
                     KMNDRGKIIEMGLWMVAMSVIGLICAIICQYYASVASQGFGTELRNQLIKKINTFSHN
                     ELNHFGTDTLITRMTNDINQLQLALAMVIRLLIRAPFLSIGSVVMAFVIDWEVGLFFL
                     ALLPIFSIILFFIIKKTVPLYQKVQEKLDQLNEHVSQNLSGVRVIRAFAQTKKETEKF
                     DKSTNDLANNYLRVSNISALLSPATTLIMNVGIICLLTVGGIKVNIGSLQQGQVLALI
                     NYMNQMLLALIVVSNLVVIFTRAEASGNRVKEVLDTENSILEAEVSTTPDFSSEAIIA
                     FDHVDFRYTPDSGLSLQNITFQLKRNTVLGIIGPTGSGKTTLTQLIPRFYDVSSGTVI
                     MDGMDVRSWQLDHLRQQIALVPQTSVLFTGTIRENLQWGKKDATEKECWEALKIAQAE
                     EFVRQLPNGLDTKVMENGKNFSGGQKQRLTIARALIAKPALLILDDSLSALDYQTDLD
                     LRKALQTYLGSTVIIISQRVRSIQEAHHILVMDQGKIAAQGTHEELLAQSAEYQEIVA
                     SQEEE"
BASE COUNT         3894 a         2276 c         2514 g         3737 t
ORIGIN      
        1 attcaaaggg ggtatgacac tagttgtgtc ataccccctt tttccgaaat ttgagaagca
       61 attttcgtaa attccttctt attacttgaa gccaatcata taatacagaa acagagaact
      121 atccaaaata aatcctagaa aaagaagtac gagcttcgct gatttttgtt aatttagaat
      181 aatactaata ggaatcgtaa aaacagaaaa aaagcataat gataaagggt cgggcagatt
      241 ttaattgatt catctggtac tcttttttat tgtccttaca ttcataaagt ttgaatgcaa
      301 tgactttatt acatctatat ttatatctaa cagtagcgtt gatgtgttaa ttataagttc
      361 tatcacttta tgttcaatta gtttgtgctc ttatatgtgt gattgaaaat atagataaaa
      421 aaacaatcat cggctttgac ttcgatgatt gttttcttta ttttgtaaaa atcctatcac
      481 ttgattttat ggtaaccttt tatttttgtc ttcaaacggc ttggtaagag agcagtaagc
      541 agtgcaaatg ggatcgaaaa gacataccag aaaatcgtac gtccgatacc ataatgttga
      601 tagagctttg tcaacatcac gtgtaacaag atcaacccta aaagcaagga gccagctata
      661 aaccaatgat tatcccaatg catgacttca ctaagtgcca tcactccaaa aggatagaaa
      721 taaaaataga tcccgactaa ggtcatccgc cagttctttg taaatgttcc gatattcaat
      781 aaaaagaaaa tcgctgaaag caaaataccg ataggcggaa aaagcccaac caaaaaggaa
      841 tacaacatcc cccacaagat catcacagga cctttgatca atgcaacgat tgcgatgaac
      901 aaagtcagca atattagttt ttgtgataac gttgcaacag tcattaatcc aaataaaatt
      961 aatgtgaacc aatgccaatg ggactgctgt tgttctttac gggaaaaatc gacccggttg
     1021 gataaataat ctgctaattt cggataagaa cgcttaaaat ccataaactg ttcctccttc
     1081 gtggaaaagg taggtttagt atacgaaaaa taaaaaagaa atacatccgt cttaagaggg
     1141 agtttttcac tgaaagtgga gaaaaaagaa tggtaaatta aaatattctc agttatactg
     1201 aaaattatac caaatttcaa agaagaaaga ggatgggaat gaaaggcaaa ctaaaacgag
     1261 aaatcaatct gtttggcgca ttagctaccg ttatgggaac cgtgatcggt gcaggcgtct
     1321 tcttcaaaac tgctgctgtt actgctagta ctcagtctac aagtttgaca ttgctagctt
     1381 ggcttttggg tggtttctta acgatctgtg ctggattgac agtggctgaa ttagctacag
     1441 caatcccgga aactggtgga gctgtaaaat atattgaagc tgcgtatgga aaattgccca
     1501 gctttttatt aggatgggcg caaagcttga tttactttcc tgctaatatt gctgcattat
     1561 cgattatctt tgcgacacaa ttgacgaact tattacaatt atctaccgat tatcttttgt
     1621 tgattgcagt gatcaccgct gtttctgtga cagggttgaa tttgttaggg acaaaagtcg
     1681 gtgcttctgt acaatcagtg acgttgattg ttaaattaat accaattgct gtgattgtca
     1741 tatggggatt attgacccca ggacaaggaa ctgtccagtt atttccaatc gaagctggaa
     1801 aagatgttac ttttgtcgaa ggattgagca gtgcgttgtt agcaacgttg tttgcttatg
     1861 acggctggtt aggtgtcggt gcaatggcag gggaaatgaa acggccggaa aaagatttgc
     1921 ctaaagcaat tattcttggt ttgagttttg taacagtcgt ttatctattg atcaattttg
     1981 tttttttgaa aactttgccg attgatcatc ttgccggtaa tttgaatgca gcttctgaag
     2041 catcagacgt catttttgga ggaatcggtg gaaagctggt cactattggg atcttgatct
     2101 cggtatacgg tgcattgaac ggttatacac taactgggat ccgcgtgcct tatgcgatgg
     2161 ctttagaaga cgagctacct ttcagtaaac agctgacaaa gctttcaaag aagtttacgg
     2221 ttccttatgt tcctgcggtt ttccagttag cagtagcttg tattatgatg agccttggtt
     2281 ctttcgactt tttaacagac atgttgattt ttgtgatgtg gctattcagt ttactgattt
     2341 ttatcggtgt ctttatttta agaaaaaaag cgccggaatt gccacgtcca tataaagtac
     2401 cactttatcc aatcgttccg attattgcga ttctcggtgc tattttcatt ttaggtatga
     2461 cgatgatgac gcaaacgaaa ttagcattga tcggtatagg tgttaccttg ataggtatcc
     2521 cagtctatta tcaaaaaaag aaaaaacgct cagaagagta aaatatttca acaacagaca
     2581 gaaaaagtac gaactaggtc ctctagatct tcgtgctttt tctatttcaa taaaacaagt
     2641 tcttctattt tgatttttgc tacttctgca agtagtgctt atgtgctaaa gtaagaatga
     2701 aaaggagaga taaacatgat atttgattct catacccatt taaatgctga acagttcaat
     2761 gaagatattc cagaaacgat tcagcgagca caagaaatgg gggtaacgaa aatggcggtc
     2821 gtgggcttcg atacgccaac gattgaaaag tctttgcaat taagccatga ctatccaaat
     2881 atatacagta ttatcggctg gcatccgaca gaggctggca gttatacgaa agaaatcgaa
     2941 aataaattac aagaacaatt gactttacca aaggttgtag cattagggga aattggcttg
     3001 gattatcatt ggatggaaga cccgaaagaa gtccaagata aagtatttcg cagacagatt
     3061 gctatcgcaa aagaaatgaa cctaccaatc agtattcata ctagagaagc acttgaagac
     3121 acgtatcaaa tcctaaaaga agaagatgtt cgagatatcg gtggaatcat gcatagtttc
     3181 agtggtgatt atgaatgggc aaaacgattt cttgacttag ggatgcatat ttcatttagc
     3241 ggtgtcgtca ctttcaaaaa agcattagac gtccaagagg ctgcagcaaa tgtaccaatc
     3301 gaacgattac ttgtggaaac agatgcccct tatctggcac cagttccttt tagaggaaag
     3361 cgaaatgaac caggctatac tcgttataca gtagaaaaga ttgctgaact tcggaatctt
     3421 ccgacagaag aagtggctgc ccaaacatgg acaaatgcgc accgattatt tggtctaagc
     3481 gaacatggat aagatgaaga tccaagagat tatagtcgtt gaaggaaaag acgatacaag
     3541 aaggctgcgg gaagtagttg atgcagatac gatagaaaca atagggtctg caatcaatga
     3601 agagatcctg atgcaaattg aacatgccca agagactcgt ggagtgatcg tttttaccga
     3661 tcctgatttt tcaggagaaa aaataagaaa aatcattatg gaagctgttc ctcaggcaaa
     3721 acatgctttt ttgccgcgca cacaagctaa accaaagaaa aagggaagcc ttggtgtcga
     3781 gcatgcaagt gatgctgcta ttttagaagc attgaaaaag gtcgtgacgc cagacatttc
     3841 ccaaacggct gtttctgaaa tcactagaca agacctttta gactatggtt taatagctgg
     3901 ggcacaagca aaagaaaaac gagaaaaact aggtgacgag ttgcgcatcg gttacacgaa
     3961 cggcaaacaa ttggaaaaac gtttgaagat gtttcgtatt acgccgaaag aactcgccca
     4021 agcaatgaaa atagtggagg aacaacatga gtgaatatcg tgaaattgcg acaccctcta
     4081 gaacaaaaga gattttattg aaacatggtt tttcatttaa aaaaagttta ggacaaaatt
     4141 ttctgactga accaaatatt ttaaggaaaa tcgtagaaac tgcgcaaata gatgaacata
     4201 cgaacgtgat cgaagttgga ccaggaatcg gtgcattgac ggaacaatta gccaagcatg
     4261 caaaacaagt cgtagcattt gagatcgatg atcgtttgat ccctgtattg gctgatacga
     4321 tgcaacccta tgacaatgtg actattgtac atcaagatat tttaaagacc gatttgtcaa
     4381 cagctgttag agaaacattt catgaggagc ttccgttaaa agtcgtagcg aatttacctt
     4441 actatattac gacaccgatc atgatgcatt ttcttgaatc agatttggtt gtggatgaac
     4501 tagtcgtcat gatgcaaaaa gaagtagctg atcgtatctc tgctgaacca ggaacgaaag
     4561 cttatggttc attatcgatt gctgttcaat attatatgga agccagttta gcattcatcg
     4621 taccaaaaac agtatttgtc cctcagccaa atgtagattc agcgattttg aaactgacaa
     4681 gaagagatat accggcagtc gaagtgacag acgaaaaagc atttttccga ttgacaaaag
     4741 cggcatttca gcaaagaaga aaaacgcttt ggaataactt gcagcattcg tatggcaaag
     4801 atgaccagac aaaagcatgg ttagcaaaaa gcttggaaac agctggaatc gatccaaaac
     4861 gtcgtgggga gactttgtca ttacaagaat tcgccgcatt aagcaatgcg atgtcagaaa
     4921 atatgcagta aaaaaagagg ttgtgacaga agttgtgaaa aagctgtcct gcatcaagta
     4981 ataagaacag ccaaacaaaa atagttcctc atgtttttcg tttgtttggg cttattaccg
     5041 cagatgcaag cttttgaaca ccgtttaaga aagaggttgt gggcctgccg tttaactcta
     5101 agtcacaagg aacaatttca aaaaacagct tctcatgttt ttgagttagt tggtcttatt
     5161 acttgatgca agcttttgac cactattatg ccgaacaagg agatgtgaca caaattttgt
     5221 cacatctcct atttttgttt cttgatcaaa aaattcttat acttgatact cacactcatt
     5281 ttgtatggat ttagaaaaga atatgtgtct tgacagttat tagtaagtga gttatactaa
     5341 gtcgacattt atgtgtcaag acatattagg agagagaaaa aatgagaaaa aattcagttc
     5401 gaaaactaac gatttctgct ttattgattg ggatgggggt cattatccca atggtcatgc
     5461 ctaaaatcat gattccacca gcttccttta ctttggcgtc tcatgtgcca ttatttattg
     5521 cgatgttttt tacaccagga gttgcagtgg ctgttgcgct aggtacgaca tttggtttct
     5581 ttttaacaac gccggttatc atcgcattgc gagcattgtc acatcttgtg tttgcgttga
     5641 ttggggcttg gtacttgcaa aagcatccga gtatcgtttt aaaaaatgga cagtttactt
     5701 ttgctaattg gcgtttccaa gggttcaatt ttgtgatcgg tgtgattcat tctttagcag
     5761 aaatgctagt agtcagcatc ttttacttta ttggtaatat gcctgaaaca tattattctc
     5821 aaggatattt ttatacagta ttcatcttga tggggattgg tgggcttatc cacagtttag
     5881 ttgattataa tattgcttat tttatagcag gaacactcag caaatatttt gatatccccg
     5941 tatttacggc agcaaaaaaa gaaaatcgga aagagtcgat tcaagggtac gcgaagaaaa
     6001 cgacatatta gacatgatct ctcaaaaaaa aaactctcat ctagtaatgg ataataaatc
     6061 ctctaagatg agagtttttt gtcctaaaaa gagctgcacc aacatcttct ggcacagctc
     6121 taacaggggg caatttatgg aagtttgatg acttgttctg tttctttatc aaagaagtgg
     6181 cctttgctga tgttgaaggc aaggtcgact atttcgcctg gtttatggaa atctcttgca
     6241 tctacttttg agatgaactc agtagaacca gtttttgtgt aaagcatcgt ttcagctcca
     6301 agtaattcgg aaacgacaac ttctgatttc actacggctt caggtgaagc ttcaagggct
     6361 acttgttcac tgtggatgtc ttctggacga ataccgaaga taacttcttt tccttcataa
     6421 cctttttcaa ctaatacttt gttttttcct tcaggaatac gaagcttaag cccatgtcca
     6481 tctgtgataa caccattttt caatgtcaca ttaaagaagt tcatggcagg agatccaata
     6541 aagccggcaa cgaacatatt gacgggtgta tcataaactt ctttcggtgt accgatttgt
     6601 tggataaatc cgtctttcat gataacgatt cggtcagcca tagtcattgc ttctgtctga
     6661 tcgtgagtaa catagatggt cgttgtttca agacgctggt gaagtttggc aatctcagca
     6721 cgcatggcta cacgtaattt agcatctaaa tttgataatg gctcatccat taagaagact
     6781 tttgcatccc gaacgattgc ccgtcccaat gccacacgtt ggcgttgtcc accagataaa
     6841 gcagctggtt ttcgtttcaa gtattctgtc agacctaaga tctctgctgc attgtccaca
     6901 cgttttttga tgtctgcttt atcatattta cgtaatttca agccaaaagc catattatca
     6961 aaaacagtca tatgaggata caatgcgtag ttttggaaaa ccatggcaat atcacggtct
     7021 ttaggtgcca cgtcattcat gacttttccg ccgatattta gttctccttc agagatatct
     7081 tccagtcccg cgatcattcg caaagtagtt gactttccac aaccggaagg accaacaaaa
     7141 acgataaatt cacggtcttt gattgataga ttaaaatcag ttacagaata attttctgca
     7201 ttgtcatatt ttttatatac gtttttcaga gccatttcta ccataatacg ttccacactt
     7261 tcttttttag ttttattaac tatttaaagt ataaatgaaa gcgttaacta acttcaatgt
     7321 catgttgaac aagaattttt tctttttttt gtcaatgtgc tagttttaag ctttaataaa
     7381 tatccataag aaataaaaac tgatacactg gagtaaataa gtatgttaac aatatcaaaa
     7441 ggagtgtctc ttatgaaaat cgctttgatc gctcatgacc gaaaaaaacc tatgatggtg
     7501 aaattagcaa cagcgtataa aacaatattg gaaaaacatg aactttttgc taccgggacg
     7561 actgggcaga ggatcacgga agcaaccggc ttgccgatcc atcggtttaa atccggaccg
     7621 ttaggcgggg atcagcaaat tggagccatg atttcggagg atcagttaga tttggtgatt
     7681 tttcttcgag acccgctggc tgcacagccc catgaaccag atgtaacagc acttatccgt
     7741 ttatctgatg tctatgagat tccgttagct acgaatatcg gaacagctga ggtgctacta
     7801 agaggactgg aagccggctt cctagatttt cgaagaatcg tccatgaaat agacaagcat
     7861 ccactttcat tttgatggat aagcgtatgg aaaaataacg tttaatgttc gcctatatgt
     7921 agctataaaa gctaaacaaa ctgataatat gcgatataat acgaacgtat tggataatat
     7981 aaacaataat attagttttt cgtatttgca gtaaatgtga aaatatgcta tagtttctgt
     8041 gggaaaagat cccaaaaata atacagagta gaaaataaag cgttaagtgc taagaggatg
     8101 ggatgttgcc tcttggacga aggacgtaat gttcgcggtt ttattttcgc attcgctgca
     8161 tgatacgatg tagcagctct taaaggagat gctaaaaatg aagaaacacc ggttagacgc
     8221 acgtatgatt gctattatgg gcttattgat tgcattgatg gtcactttgt cccgactggt
     8281 agcaatcgag accccattta ttaaaatcag tgtcacattt attccacaag tgatcatggg
     8341 cattcttttt ggtccttttt ggtcagggat aggcgcagtt ttagctgatt tagtaggaat
     8401 ggccctcttc tcgaaatctg cattctttat tggatttaca ttgaatgcat ttattgaagg
     8461 agcaatctac ggattctttt tctatcgaaa agagatcact tggaaaaatg ccattctggc
     8521 tacgcttagt gtcacgctga tcatcaactt atttttgaca ccattatggc ttgcgttgat
     8581 gtaccatgtg ccattgttca gctgggtcgt ttgggcgccc cgcctgttaa aaacagtaat
     8641 atggttaccg attcaatcaa ttgccattta ttacgtagga cgtagtattc cttacaaaaa
     8701 aattttaaga agtttagcga tccacgctaa ataaaaaaca agcagtcttc ccatcccaga
     8761 agactgcttg tttttattgt gctgtgaatt gtgcttgata gagccgtcgg taataaccat
     8821 ctttttgaag cagctcatca tgggtgccca tttcgacaat agctccctga tccatcacta
     8881 atatctggtc cgcatttttg attgtggaca ggcgatgggc aatcacaaaa ctggtttttc
     8941 cttccatcat tgctaagaag gcttgttgga tttttttctc tgtcaatgta tcgactgagc
     9001 tggtagcttc atctaagatc aacatagggg gattactgat catcgttcga gcaatcgtca
     9061 acagttggcg ttgtccatct gatatcttta acccttgacc gccaatctta gtatctaatt
     9121 tttccggcaa acgttctaca aattcataca tataagaggc ttttagggca tcattgattt
     9181 ctgcgtcact tgcttccgga ttcccaaaac gaagattttc tcgcaatgta ctgtcaaata
     9241 gccaagtatc ttgtaacacc attccaaaac ttttacgtaa tgtatcacgt tggatagagc
     9301 ggatgtcatg gtcatcgact gtaattgttc cttttgttgt gtcataaaaa cgcatcagca
     9361 aattaacaag tgtggatttt ccggcccctg tttttcctac tatagcaatg gtttgacctg
     9421 gataagcagt aaaattgaaa tcttcgatca aacgctgctt ggggtcataa gaaaaagaaa
     9481 catgatcaaa agagactttt cctttgatat ttttgggttc caaggcatag gcatctggca
     9541 aatcaggctt ttcttctggc tgatcgatca aatcaaatgc tcgttctaga ccagaaaaag
     9601 cggtttgcag ttgtgtcgtg attccagaaa gttcgataaa tggtttagaa aactgacttg
     9661 agtagatcgt aaagctggaa atcacaccaa cagtgattga cgaaccactc ttcaatgcca
     9721 ataggccgcc aactaaacca atcgataaat aagcaaggtg atctacaaaa cgagaagacg
     9781 ggtttgtcaa ggaagaagaa aattgtgccc gttgtccttt ttcatataga tcagcattta
     9841 gcagttcaaa ttgctgttga ctagcttctt cttgctgaaa agctttcacg attttctggt
     9901 taccgattcg ttcggtcaca aagcccgaga tatccccaat aatcctttgc tgtctatcga
     9961 aatttgtttg tgaagcacga gcaacgatcc aactcacaag gaaaataatt ggtgtagaac
    10021 aaagaacgac caatgtcaga agtggactta gacgcatcat aaaaataaac gaaagaatga
    10081 tgattgccac tccagaaaat aattgattga aggccgcaga aattgcaatg gaaatattgt
    10141 ccatatcatt tgtaaatcga ctgacgatat ttccgtggga gttttgatca taataacgaa
    10201 gtggcaatcg attcagatga gcaaatgcat cttttcttaa ttttgcaacg gataagtaag
    10261 ctaaccgatt gcttaacact tggatgagcc attgactgac tacgttgatt aaaacgatac
    10321 caccgaatag aataagtaaa tggaacagtc gattgaagcg gacctgtcct tctccaatca
    10381 tttgatcgat actttgacca atttgtaacg tcatcacaac tgttgcaatc ccattgatga
    10441 ttcctagtat cacagctagc caaatgtctt ttgcataggc agtaaggtaa ggaatgaatc
    10501 ggcggagcgt ctgcaaggat aattttttta ctttcataac tattcttcct cctgtgatgc
    10561 gacgatttcc tgatattctg ctgattgagc aagtaattct tcatgggtac cctgagcagc
    10621 aatcttacct tgatccatca ctaagatatg atgtgcttct tgaatagagc ggacacgttg
    10681 ggagataata atcactgtac tgccaagata agtctgtagc gccttacgca aatccaagtc
    10741 tgtttgataa tccaacgcac ttaaagagtc atctaaaatc agtaatgctg gcttggcaat
    10801 caaagctctt gcaatcgtca gtcgctgttt ttgtccgcca gagaaatttt tcccattttc
    10861 catgactttt gtatctaaac catttggaag ctgacggaca aattcttctg cctgagcgat
    10921 tttcagagct tcccaacatt ctttttctgt ggcgtctttc ttaccccatt gaaggttttc
    10981 acgaatagtc ccggtaaata gtacagaagt ttggggaaca agagcaattt gctgacggag
    11041 atgatccagc tgccaagaac gaacatccat tccgtccatt ataactgtac cggaactgac
    11101 atcatagaaa cgcggaatca attgtgtcaa agtcgttttc ccgcttccgg ttggaccaat
    11161 gattccaagg actgtatttc gcttcagctg aaatgtaata ttttggagag aaagaccaga
    11221 atcaggagta taacggaagt caacatggtc aaaagcgata attgcttcag aagaaaaatc
    11281 gggagtagta ctgacctcag cttcaagaat cgaattttct gtgtctagca cctctttcac
    11341 ccgatttcct gaagcttcgg cacgtgtgaa gatcacgacg aggtttgaaa caacgatcag
    11401 tgccaaaagc atttgattca tataattgat caaagcaaga acttgtcctt gttgcaagct
    11461 accaatattt actttgatcc cgccaactgt cagcaaacag atgatcccga cattcatgat
    11521 caaggttgta gcaggtgata atagggcaga gatattggaa acacgtaaat aattgttcgc
    11581 tagatcattt gtgcttttat cgaacttctc tgtttctttt tttgtctgcg caaatgcgcg
    11641 gatgacacga acaccgctaa gattctgact aacgtgttca ttgagctgat ctagtttttc
    11701 ttgcactttt tggtagagag gaacggtttt tttgatgata aaaaataaaa tgattgagaa
    11761 aataggcaat aatgctaaga aaaacagacc tacttcccag tcgatcacaa aagccatgac
    11821 cactgacccg atacttaaaa acggtgctct gatcaataaa cgaatgacca ttgctaaagc
    11881 tagctgaagc tgattgatgt cgtttgtcat tcgggtgatc aaggtatctg ttccaaaatg
    11941 attcaactcg ttgtgagaga aagtattgat ttttttgatt agctgattac gtaactctgt
    12001 accaaatcct tgtgaagcta ctgaagcata gtattggcaa atgatagcac aaatcagacc
    12061 aatcacagac atagcgacca tccataagcc catttcaatg atttttcctc ggtcattcat
    12121 ttttagtcca ttatcgatca aggaagccat cattaaaggc aggacaagtt cgaagacagc
    12181 ttcaagaaat ttgaagaacg ggcccaaaat gatttgctta cgatagtctt ttgcataacg
    12241 taataactga atcatatgat tcctcctatt atttatttgt ccacaattga aaaaacaagt
    12301 actaccacat tgtagcttag gaaatttaga atgaaaagag aacaagcctt ttcataaatg
    12361 aaagttttat tatgaagaag ctttagaaat tgaatagact atttttttta aaaaaaagaa
    12421 a
//
LOCUS       DAZKLD010000091        12294 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.091, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000091 DAZKLD010000000
VERSION     DAZKLD010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12294
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.091"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            141..461
                     /locus_tag="ACG8KR_001978"
     CDS             141..461
                     /locus_tag="ACG8KR_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0201337.1"
                     /translation="MKKRLLWCLLFLSLSLTSQPVFAEETQRSPITGTRLYKNVYVQT
                     TDLDSAIPETEERSLQPRFGYFWYCPTCDYESCWHFLHSTAAAFAFTHTQKYDGHHPV
                     IYEV"
     gene            913..1113
                     /locus_tag="ACG8KR_001979"
     CDS             913..1113
                     /locus_tag="ACG8KR_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFQ0201338.1"
                     /translation="METGTVKWFNSEKGFGFITAENGNDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEEGQRGPQATNVTKA"
     gene            1260..4049
                     /locus_tag="ACG8KR_001980"
     CDS             1260..4049
                     /locus_tag="ACG8KR_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase C-terminal domain-containing protein"
                     /protein_id="HFQ0201339.1"
                     /translation="MKPRTYAVVDIETTGTNPKEDRIIQFGCVMIESGRITSRFSIDI
                     HPGRKISKQIQHLTGITNQRVQKAPYFEDVAQTIYNLLADTIFVAHNIYFDYNFLNHE
                     LMRCGLPSLKIPGIDTVELAQIFLPTEPSFRLADLSESLGFIHENPHQADSDAEVTGQ
                     LLLLIEERMRKLPIITLEQIARLSRHTGMDTSRFIYHVIEEMKEKPEPLDPSLEIVDG
                     LALQKKEVELFTSVHYGERTYPRKKQAKEKMFGKTLMYRKEQNRLMNAVYDHFTKDES
                     KDLMIEAATGMGKTIGYLLPLSYLATPEKPAVISTVSLVLQQQIIEKDIPLLNQLLDQ
                     PIQPVIIKSYRHYIDLQRFKGTLVEPPEQKQYALYQMAVLVWLTQTKTGDLDELHLTN
                     LNHSFFADIAHRGIGFLARNQSFYEQDFVRFLQKKIRQSNFLIVNHAFLIQETQRKEP
                     LLPASPFLLIDEVHHLPEIAEKVNDKRLSINYFHKQIQQLEEPNQLFDRIQSYLPKEH
                     EALRWLSIYREELSALCEAQICMAEGLMSLVSGKQPNGQYPEEVMMTKEMLDQLPLES
                     GTAIDHLQLFYQEIQQLQSQMRLTFEIIQESWTNQMRIDLASLYSLFDQLDKQYLLMD
                     QWLEDWRDHLIHWFVPGNTAQQMIFHLHDFQAAMLPSTVWYPRYDRILYIGGTLKIGP
                     NRHYLSKRLGIEAAPLKVIASPYDYEKQARIYVPKEAPAVHQVSNQAYIDYLETALTG
                     LIEQEDRPILVLFTSHDVLQKVYQKMHLSMLEKGREILAQGFGGSREKLLKRFILSEK
                     SVLFGADSFWEGVDLPGDALQIVVVTRLPFDNPLRPMVKARNAYLESEGINPFYQESV
                     PKAALKLRQALGRLIRTETDRGVLLILDRRLVTAKYGARIIHALPKKVPIKELAMEEI
                     YQDIHEFLKKNEEGS"
     gene            4096..4623
                     /locus_tag="ACG8KR_001981"
     CDS             4096..4623
                     /locus_tag="ACG8KR_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5590 domain-containing protein"
                     /protein_id="HFQ0201340.1"
                     /translation="MKIKERRPLKKKQTKEKTINRVLLGLIVFLLAVIVISSIFYLRS
                     SRPAVQAKKEAVEIAKKYANLEEADHFYWFTRKKTYFSLTGTNDKGKEIAVIIPKSGE
                     KVKVLDQTKGLTENEAKQTIAAAHPEIQIEKAALGMYDDQPVWEVVGKETNGKLNYYL
                     IGFESGDEVKTIKGI"
     gene            4644..5834
                     /locus_tag="ACG8KR_001982"
     CDS             4644..5834
                     /locus_tag="ACG8KR_001982"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFQ0201341.1"
                     /translation="MELSKRTKRLEPSVTLAAAAKAKALKAKGRDVLSLTVGEPDFAT
                     PENIQEAAIEAIRNGKASYYTPTAGIPELRQAIVDYLKKNYGLVYEPSQTVVTDGAKF
                     ALYTLFQTILDPQDEVIIPVPYWVSYGEQVKLAEGLPVFVKGEESNSWKVTVEQLEQA
                     RSEKTKAVIINSPSNPTGMIYSKNELQAIGEWAVKNDILIVADDIYGRLVYNGNEFTP
                     IATISEAIKNQTIIINGVSKTYAMTGWRIGYAVGNPVIINGMIAVASQSTSNPTAVSQ
                     YAAVEALKGEQDTVEEMRNAFEERLNTLYSLVAELPGVSLEKPQGAFYLFPNVKETLR
                     MCKYENVTQWVEDLLEETGVALVTGEGFGAPENVRMSYATDRATLEEAVRRIKQFIES
                     KSQN"
     gene            5874..7172
                     /gene="asnS"
                     /locus_tag="ACG8KR_001983"
     CDS             5874..7172
                     /gene="asnS"
                     /locus_tag="ACG8KR_001983"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HFQ0201342.1"
                     /translation="MEQIQIIDSKNHVGETVTIGAWVANKRSSGKIAFLQLRDGTAFF
                     QGVVVKSEVSEEVFQTAKNLHQETSVLVTGEIREDSRSKFGYEIGITNVEVVSESHDY
                     PITPKEHGTDFLMDHRHLWLRSSRQHAIMQIRNEIIRATYEFFNQHHFVKIDPPILTG
                     SAPEGTTDLFETNYFDQKAYLSQSGQLYMEAAAMAFGKVFSFGPTFRAEKSKTRRHLI
                     EFWMIEPEMAFTHQEESLEVQEQYVAFLVQNVLDHCEYALDVLGRDKEVLKTYTKLPF
                     PRITYDDAIELLKKNGFDDIEWGEDFGSPHETFIANSFEQPVFILNYPKAIKPFYMKP
                     HPTREDVVICADMIAPEGYGEIIGGSERATDYDFLLEQIRNHGLDEKEYSWYLDLRKY
                     GSVPHSGFGLGLERTVTWIAGIDHVREAIPFPRLLNRIYP"
     gene            8225..8899
                     /locus_tag="ACG8KR_001984"
     CDS             8225..8899
                     /locus_tag="ACG8KR_001984"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HFQ0201343.1"
                     /translation="MKKRILFIGDSITDAGRDRAEATDLGKGYPLLIAGKLISQYPTM
                     EFECMNRGIGGDKIADLQVRWQEDCLALQPDYVSILIGINDTWHNTSDQAIFGTEKSA
                     QQFESVYRKLLNELQTVGICNIILMEPFVLPITDDRHGWRKDLDPKIQIVRKLAKEYG
                     ALLVPLDGIFNALGIVYGFSKYTREDGVHPTIGGHEIIAQSWIDAIAAVDFFSGDKNE
                     RKKSEK"
     gene            8896..9237
                     /locus_tag="ACG8KR_001985"
     CDS             8896..9237
                     /locus_tag="ACG8KR_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0201344.1"
                     /translation="MMEIRWAVKEDLPQINQLITDHPESFVKAKTAADLNDKNKLLLV
                     AVTKEKRIIGFAQADVQQSEERLDQRPSGKLIQHTLVTKENTEEIQKKFAEYLILEAK
                     KRRIKSFFAQF"
     gene            9267..9626
                     /locus_tag="ACG8KR_001986"
     CDS             9267..9626
                     /locus_tag="ACG8KR_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HFQ0201345.1"
                     /translation="MSCLGNVIWFIFGGLIGGLSWLAAGILWCITIIGIPIGMQCFKI
                     ASLSFAPFGKEVIYHQSGMSLIVNIIWLIVSGLPIALAHVVSGILLCITIIGIPFAKQ
                     SFKLARLALMPFGAEIV"
     gene            9886..10008
                     /locus_tag="ACG8KR_001987"
     CDS             9886..10008
                     /locus_tag="ACG8KR_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0201346.1"
                     /translation="MNLDKLPATGFKLSCYPVKIKKASAGWIRAVAMIEKKKKE"
     gene            10150..12231
                     /locus_tag="ACG8KR_001988"
     CDS             10150..12231
                     /locus_tag="ACG8KR_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFQ0201347.1"
                     /translation="MKSYRLEGLDCANCAMKIEKGVQKINGVKEATVNFTSGKLTIDA
                     EEDHLATIEQEIKKVVKELEPDVKVTEIDKEKVSEHGNEKERNTLFRILFSLAGIALL
                     LLFDFNEPIRLIGYLLIYLLIGYDVVKKAVMNIVKGKIFDENFLMSVATIGAMLIGEY
                     PEAVAVMLFYQIGEYFQGLAVSHSRKSIRELMAIRPETAHVQTTEGLMTVNPEDVQIG
                     QFVLVKPGERVPLDGTIIEGESLVDTSALTGESVPKSVYVGETVLSGFINKNKPFLVQ
                     VEKSYENSTISKLLELVENASSKKAPAENFITKFARYYTPVVVGLAVLLAVLPPLVVS
                     GAAFSEWIYRALTFLVISCPCALVISVPLSFFGGIGGASKIGVLVKGSNYLELLAQTE
                     TVVFDKTGTLTKGDFSIQTIDTKIDPKIFMQYVASAEQFSTHPIAQSVLEGYAGPLLP
                     TANIQEFAGEGILAEVDGKQVLAGNHKLMERFEISFPSSQEIGTLLYLAIDQSYSGYL
                     VIADTLKEDAVDALVQLKKAGVKNTVMLTGDSKKIADHIGKQVGVDKIYSELLPEDKV
                     QRLEEILQRNNKKTAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVIMNDQPS
                     KIAEAIHLAKKTLKIVKQNIVFAIGIKILVLALGAFGFASMGDAVFADVGVTVLAVLN
                     AMRSLHVKNKR"
BASE COUNT         4087 a         2147 c         2661 g         3399 t
ORIGIN      
        1 aaaaaagagg ttgtgacgtg gtcacgacct ctttttgacg attatgatac aaaaaacatc
       61 atttatgaac aaaatgtgaa taacgggcgt tcttgagata tgattttaat tgggaaaaac
      121 ttatcgataa ggaggtcttt atgaaaaaaa gactgttatg gtgtttgctg tttctatccc
      181 taagtttaac aagtcagcca gtttttgctg aagaaacaca gcggtctcct attacaggca
      241 cacgtctgta caaaaatgtt tatgtacaga caacagatct tgattctgcg attcctgaaa
      301 cagaagaacg gtctttgcag ccaagatttg gttacttttg gtactgtccg acctgtgatt
      361 atgaaagttg ctggcatttc ttacattcta cagctgcagc ttttgctttt actcatactc
      421 agaaatatga tggacatcat cctgttattt atgaggtata gatatgagca aaaaagaaaa
      481 actaatctcg ttgatcgtat tggtttgcct tgttcttctg gtaactgtca taggctatca
      541 gaaataccat gagaacatca gacctactaa agaatcttcc ccagcctcga ccgttgaaac
      601 agaaccgata agtagtcaga aagaataatt tttttagttt aaagaagaac atcagagcaa
      661 aaaaacacca actgaacaat atgtcgttta ttttaaaaaa agttcggttt ttcactttat
      721 gtaatcgctg agaaaacttt ttttaataaa aaaagtcgaa aaactctagc ttttttgtaa
      781 acggtatgat atactatagg agtagttttt gttcggtaat ctgtggtagc ttgtttcaaa
      841 taaacatctt ccagatatac accattcaag taattgcaaa atatgtgttg atacacaagg
      901 aggattttta ttatggaaac aggtacagta aaatggttta actcagaaaa aggatttgga
      961 ttcatcactg cagaaaacgg aaacgatgta ttcgttcatt tctcagctat ccaaggcgac
     1021 ggattcaaaa ctttagaaga aggccaagca gtaactttcg atgttgaaga aggccaacgc
     1081 ggacctcaag caacaaacgt tactaaagct taattaacct atactaaaaa ataaacgccc
     1141 agacatttgc gtgtctgggc gtttttgtct attttcttta aaaagcgatg agagtatttt
     1201 tctgcacttt gtagtataat ttcaaaagaa cattagaggc aggagaagag gaagatattt
     1261 tgaaaccaag aacttatgca gtcgttgata tcgaaacgac tgggacgaat ccaaaagaag
     1321 atcggatcat ccaatttggc tgcgtcatga ttgaatcagg tcggatcact tcacgttttt
     1381 cgatcgatat tcatccaggg cggaagattt caaaacaaat ccagcattta acagggatca
     1441 cgaatcaacg cgtccaaaaa gcgccttatt tcgaagatgt cgcccaaacg atttacaatc
     1501 ttttggcaga caccatattt gtcgcgcata atatttattt tgattataat tttttgaacc
     1561 atgaactcat gcgatgcgga cttcctagtt tgaagatacc aggcatcgat acagtagaac
     1621 ttgcacaaat tttcttgccg acggaaccaa gctttcgctt agctgatttg tcagaaagcc
     1681 ttggatttat tcatgagaat cctcatcagg cagatagtga tgccgaagtg acgggtcaac
     1741 ttcttttgct gatcgaagaa cggatgagaa aattgccaat catcacgtta gagcagattg
     1801 cgcgattaag tagacacaca ggtatggata cgagtcgttt catttatcat gtgattgaag
     1861 aaatgaaaga aaaaccagag ccacttgatc cctcattaga aatcgtcgat gggctagctt
     1921 tgcagaaaaa agaagtggaa ctattcacat ccgtccatta tggcgagcgg acatatccaa
     1981 gaaaaaaaca agcaaaagaa aaaatgtttg gtaaaacact gatgtatcga aaagaacaga
     2041 acagactgat gaatgcggtc tacgaccatt tcacaaaaga tgaatcaaaa gatttgatga
     2101 tcgaagcagc tacaggaatg ggtaaaacaa tcggttatct gctgccattg agttatttgg
     2161 ctacacctga aaaacccgct gtcatcagta ccgtttcgct agttttgcag cagcagatca
     2221 tagaaaaaga cattccattg ctgaatcaat tgctagatca gcccatacaa cctgtaatta
     2281 tcaaaagcta tcgccactat attgatttac agcgttttaa aggcacactt gtggaaccgc
     2341 cagaacaaaa acaatatgct ttgtatcaaa tggcggtctt agtttggctg acgcaaacga
     2401 agaccggtga tttggatgaa ttacatttaa caaatttgaa ccattcattc tttgcagata
     2461 tcgcgcacag aggtattgga tttttagcaa gaaatcagtc attttacgaa caagactttg
     2521 ttcgtttctt gcaaaagaaa atcaggcaaa gtaatttttt gattgtcaat catgcgtttt
     2581 taatacaaga aacacagcga aaagaaccat tgcttccagc tagtccattt ttgttgatcg
     2641 atgaagtaca tcatttgccg gaaatcgctg aaaaagtaaa tgacaaacga ttaagtatta
     2701 actatttcca taaacaaatc cagcaattag aagaaccaaa tcagctgttt gaccgaatac
     2761 aaagctattt accgaaagaa catgaagcct tacgctggtt atcgatttat cgagaagaac
     2821 tttctgcgct atgcgaagca cagatttgta tggcagaagg attaatgagt ttagtgagcg
     2881 gcaagcaacc taacggacag tatccagaag aagtcatgat gacaaaagag atgttggatc
     2941 agctgccact ggaatctggg acagccattg atcacctgca gcttttttat caggagatcc
     3001 agcagctaca gtcacaaatg cgtctgactt ttgagattat ccaagaaagc tggacaaacc
     3061 agatgcggat cgatttagca agtttatata gcttatttga tcaattagac aagcagtact
     3121 tgctgatgga tcaatggctg gaagactggc gagatcattt gatccattgg ttcgttcctg
     3181 ggaacacagc gcagcagatg atttttcatc tccatgattt tcaagcggct atgttgccgt
     3241 cgactgtttg gtatcctcga tatgatcgta ttctttatat tggaggaacg ctgaagatcg
     3301 gccctaatcg acactatctc tctaagcggc ttggcattga agcagcacct cttaaagtga
     3361 ttgcctctcc ttatgattat gagaaacagg caaggatcta tgtgccaaaa gaggcacctg
     3421 ccgtccatca agtcagcaat caagcatata tcgattattt agagacagca cttacaggtc
     3481 tgatcgaaca agaagatcgg ccaatacttg tgttattcac ttctcatgat gttttgcaga
     3541 aggtctatca gaaaatgcat ctgtctatgc tagaaaaagg acgagaaata ctagctcaag
     3601 ggtttggcgg aagccgggag aagcttttga aacgcttcat tttgtcagaa aaaagcgtat
     3661 tgtttggcgc tgacagcttt tgggaaggtg tagatttgcc tggggatgct ttgcagatcg
     3721 ttgtagtcac acgtttaccc ttcgataatc cgcttcgccc gatggtcaaa gctaggaacg
     3781 cttatttaga atcagagggg atcaatcctt tttaccagga atccgtacct aaagcagctt
     3841 tgaagttacg ccaagcatta ggtcgcttga taaggacgga gacagatcgt ggcgtattgc
     3901 ttattttgga tcgaagacta gtcactgcaa aatatggtgc acggatcatc catgcattac
     3961 cgaaaaaagt accgatcaaa gagctagcta tggaggaaat ctatcaggac atccatgaat
     4021 tcttaaagaa aaatgaagaa ggaagttaga ggagctgtct tttttcataa ttaaaatctg
     4081 ctataatgaa aaggaatgaa gataaaggag cgacgaccct tgaagaaaaa acagacaaag
     4141 gaaaaaacga ttaatcgcgt gttacttggc ttgatcgtat ttttattagc ggtcatcgtg
     4201 atcagctcga ttttttactt gcgttccagt cgtccggcgg tgcaggcgaa gaaagaagca
     4261 gtagaaatcg caaagaaata tgcaaatctt gaggaagccg atcattttta ttggtttact
     4321 cgaaagaaga catacttttc actgacaggt acaaatgata aaggaaaaga aatcgccgtt
     4381 atcattccta aatctggtga aaaagtaaaa gtattggatc aaacaaaggg actgactgaa
     4441 aacgaagcaa aacagacaat tgctgcggcc catcctgaaa tccaaatcga aaaagctgct
     4501 ttagggatgt atgatgatca gcctgtgtgg gaagtagtag gaaaagaaac aaatgggaaa
     4561 ctgaattatt acctgattgg ctttgaatct ggagatgaag tcaagacaat caagggaatt
     4621 taaaaggtaa aggaatgaat gggatggagt tatcaaaaag aacaaaacga ttagaaccgt
     4681 ctgtcacttt ggcagctgcg gcaaaggcca aggcattaaa agcaaaaggc agagatgtgc
     4741 tgagtttgac tgtgggagaa ccggattttg cgacaccaga aaacatccaa gaagcagcaa
     4801 tcgaagcaat ccgcaacggg aaagctagct attatacacc aacagcagga atcccagagc
     4861 ttcgacaggc aatcgttgat tatctgaaga aaaactatgg acttgtctat gaaccttcac
     4921 aaacagtcgt aacagacgga gcgaaatttg ctttgtatac ccttttccaa acgatccttg
     4981 acccgcaaga tgaagtcatc attcctgtgc cttactgggt cagttacggt gagcaagtaa
     5041 aactggcaga aggacttcct gtctttgtga aaggggaaga atccaatagc tggaaagtta
     5101 ctgttgaaca gctagaacaa gcacgttcag aaaaaacaaa agccgtaatc atcaattctc
     5161 catcgaatcc gactggaatg atttatagca agaatgaact gcaggcaatc ggtgaatggg
     5221 cagtaaaaaa tgatattttg atagttgctg atgatattta tggtcgttta gtttataatg
     5281 gcaatgagtt tactccaatt gcaacgattt cagaagcaat caaaaatcag actatcatca
     5341 tcaacggcgt gtctaaaaca tatgcgatga caggctggcg cattggttat gcggtgggaa
     5401 atcctgtgat catcaatgga atgatcgctg ttgcttccca gtcaaccagt aatccgacag
     5461 cagtcagtca atatgcagct gttgaagcat tgaaaggcga gcaagataca gtggaagaaa
     5521 tgcggaacgc ctttgaagaa cgcctgaata cgttatattc gcttgtggca gaactgccgg
     5581 gtgtttctct tgaaaagcca caaggagctt tttatctttt tcctaatgta aaagaaacgc
     5641 tacgcatgtg taaatatgaa aatgtcacac aatgggtaga agatctttta gaagaaacag
     5701 gggtagcgct tgtgactggc gaaggctttg gggcaccaga aaatgtccgc atgagttatg
     5761 ctactgatcg cgctacgtta gaagaagcgg tacgcagaat caagcaattt attgagagta
     5821 aaagtcaaaa ttaatcctaa agaaattcaa ggaatataaa aggagagtca tacgtggaac
     5881 aaattcaaat catcgattcg aaaaaccatg tgggagaaac agtaacaatc ggcgcttggg
     5941 tcgcaaataa gcgatcaagc ggaaaaatcg catttttaca attaagagac ggaacagcct
     6001 ttttccaagg tgtagttgta aaaagcgaag tgtcggaaga agttttccaa acagctaaaa
     6061 atctacacca agaaacatcc gtgttagtaa ctggagaaat ccgtgaagac agtcgttcaa
     6121 aatttggtta tgaaatcgga attacaaatg tggaagtggt aagcgagagc cacgattatc
     6181 cgatcacacc aaaagaacac ggaacagact tcttgatgga tcatcgtcat ttatggctgc
     6241 gttcttcaag acaacatgcg attatgcaga tcagaaatga aatcattcgt gcaacgtatg
     6301 aattctttaa tcaacatcat tttgtcaaaa ttgatccgcc gatcttgact ggttctgcac
     6361 cagaaggcac tacggatcta ttcgaaacaa attactttga tcaaaaagct tatttatctc
     6421 aaagtggcca attatacatg gaagctgctg ctatggcttt cggaaaagtc ttttcattcg
     6481 gtccgacttt ccgtgctgaa aaatcaaaaa cacgacgtca tttgatcgaa ttctggatga
     6541 tcgaaccaga aatggctttc actcatcaag aagaaagttt agaagtacag gaacaatatg
     6601 tagcattctt agtacaaaat gttttagatc attgcgaata tgcattagat gtactaggac
     6661 gagacaaaga agtcttaaaa acttatacaa aattgccatt ccctcgtatt acttatgatg
     6721 atgcgatcga attactgaag aaaaatggat ttgatgacat cgaatggggc gaagactttg
     6781 gttcacctca tgaaacgttc attgcgaatt cattcgaaca gcctgtcttt atcctaaact
     6841 atccaaaagc catcaagcca ttctacatga aaccacatcc aacaagagaa gacgtggtga
     6901 tctgtgcaga tatgatcgca ccagaaggtt acggtgaaat tattggcgga agcgaacggg
     6961 caacagacta tgatttcttg ctagaacaaa tccgtaatca cggtttggat gaaaaagaat
     7021 attcatggta tcttgatcta cgtaaatacg gttctgttcc gcactctggt tttggtctag
     7081 gtcttgaacg aacagtcact tggattgctg gtatcgacca tgtacgtgaa gctattccat
     7141 tcccacgttt gttgaaccga atttatccat aagaggtgat gaaaaagctg ttctgcatca
     7201 agtaattaga acagccaagc aaaaacagct gttcatgttt ttgaattggc gggacttatt
     7261 accgcagatg taagcttttg aataccgttt agtggagagg ttgtgaaaaa gctgtttagt
     7321 ttcgagtatt aagaaaaatt ttgtggaaat ggctttccac atttttgcat aaatttggct
     7381 taataccgag aaactggctt ttgaacaccg tttaaggaag aggttgtgcg cctgccgttc
     7441 agctccgagt cacaaggagt aatttcaaaa aacagttttt catgtttgat gactattgtg
     7501 acttgtgacc gaggagttgg ccggagagca ccgtttattc taaaaaagaa ctgcgaactc
     7561 tcgcagttct ttttttaaaa tacgggataa aggaagtttc cagcagacaa gagaagaaag
     7621 caaaagaaat tgctatttga ttcacaaggt tctataacga aatcgcaatc aatcaaacat
     7681 ttacaaagaa ggtaattcaa atggaaatct gatttgatga tgcttctgct gttgaaataa
     7741 agcagcatct agcacctgaa aacaaactaa tagtaacatt cgaagatgga gtaggtccgt
     7801 attttcagca tgcaataatc catatgtaag ttcaattcac ccttgaaatc atcggaccag
     7861 ataaagttat gaccaatcta tcccaagcaa tctagaagag atattagtaa agggacattt
     7921 actagaagtc ttagatgagc ctataaatat atgttctgat ggcgtcatca gtggtctgat
     7981 tgacggaagc ttaggtgtga tcgatctctc tggaccaaac aacgaaggaa tcaaggggaa
     8041 tccggcacgt taagaaaaaa atagcataaa aaactagaca cttgatgagc gttcattcaa
     8101 ttgtttgaag ggactaggct ggtgtcattt tttatacata tattttctct ttcgtcttga
     8161 tagggcttat gggataatat aaggaagaaa atcatcgttt tattcaaaaa ggagtttttg
     8221 acaaatgaaa aaacgtatat tatttatcgg tgatagcatt acagacgcag gaagagaccg
     8281 agcagaagca actgatctag gtaaaggtta tccacttttg attgccggaa aactaatctc
     8341 ccaatatcca acgatggaat ttgaatgcat gaaccgtggg ataggaggag acaaaattgc
     8401 agatctgcaa gtacgctggc aagaagattg tttagcttta caacctgact atgtatcgat
     8461 tttgatcgga atcaatgata cctggcataa tactagcgat caggctatat tcggaactga
     8521 gaaatctgct cagcaatttg aatccgttta tcgaaaatta ctaaatgaac ttcaaacagt
     8581 gggaatctgc aacatcatct tgatggagcc gtttgtactt cctataaccg acgaccgtca
     8641 tggctggcgt aaagacttag accctaaaat ccaaattgtt cgtaaacttg ctaaggaata
     8701 tggcgcattg ctggttcctt tagatggtat attcaatgct ttagggattg tttatggttt
     8761 ttcgaaatat acaagagaag atggggtcca cccaacgatt ggcggacatg agatcattgc
     8821 acaaagctgg atagatgcaa ttgccgcagt cgattttttt agtggagata aaaatgagcg
     8881 aaagaaaagt gagaaatgat ggaaatacgg tgggcagtaa aagaagatct acctcaaatc
     8941 aatcagctga ttactgatca tccagaatca ttcgtaaaag caaaaacggc agctgaccta
     9001 aatgataaga ataaactatt gctcgttgct gtaacaaaag aaaaacggat tatcggattt
     9061 gcgcaagcgg acgtgcaaca gtcagaagaa cgattggatc agcgaccatc aggaaaacta
     9121 atccaacaca cattagtaac aaaagaaaat acggaagaaa tccaaaaaaa atttgcagaa
     9181 tatttgatac tggaagctaa aaaaagaaga ataaaatcat tttttgccca gttttaatca
     9241 aaaacagtaa tagaaggaga aatacaatga gttgtttagg aaatgtcatc tggtttatat
     9301 tcggcggact aatcggtggt cttagctggt tagcagcggg gatactttgg tgtatcacga
     9361 tcataggtat cccaatcggt atgcaatgtt ttaagatcgc ctccctttct tttgcgcctt
     9421 ttggcaaaga agtaatttat caccagtctg gcatgtcatt gattgttaat atcatctggc
     9481 tgatcgtttc aggcctacca attgctctcg cacatgtcgt cagcggcatc cttctatgta
     9541 tcacgatcat agggatacct tttgccaagc aatctttcaa acttgctcga ttggcgttga
     9601 tgccattcgg tgcagaaatt gtataaaaga gaataataaa ggttagataa agcatagagt
     9661 caggtgggat acatgtattt cacctgactc tatgcgttct cctgtataag ttcttccatg
     9721 tgacttttac gtcttttatt tatatggggt tactgtttat ggaaagttga aataattttc
     9781 attaaagaac tctttctata tttttgaaag cgtagtggag cttcgaatat ctgggaagga
     9841 cattttgcag ggattgaaaa aagatattgt cctatcgaaa aattaatgaa tttagacaaa
     9901 ttgccagcaa caggcttcaa acttagttgc tatcctgtca agatcaagaa ggcaagtgcg
     9961 ggttggataa gagcagtggc aatgatcgaa aagaaaaaga aagaataagc caaaacatct
    10021 gtatggaaga aattttccga tgcagatgtt tttcattttt tctttcggat accttgacat
    10081 atgaataacc gttcatttat aattagttat gaagagatat tcatatgttg aggcgaggag
    10141 gtcattaaaa tgaaaagtta cagattagaa ggtttagatt gtgcgaactg tgcaatgaaa
    10201 atcgaaaaag gagttcagaa gatcaatgga gtcaaggaag caacggtaaa cttcacttct
    10261 ggaaaactaa cgattgacgc agaggaagat catttggcta cgattgaaca agaaataaaa
    10321 aaagtagtga aggaattaga accagatgtc aaagtaacag aaatagataa ggaaaaagta
    10381 tctgaacatg gaaatgaaaa agagagaaat acgctgttcc ggattttatt ttcccttgca
    10441 ggtatcgctt tattgctatt atttgatttt aatgaaccaa ttcgcttgat agggtattta
    10501 ttgatttatc tgctgatagg atatgatgta gtgaagaagg cagtgatgaa tatcgtcaaa
    10561 ggaaaaatct ttgatgagaa ttttcttatg tctgttgcta cgattggcgc tatgttgatc
    10621 ggtgaatatc cagaagccgt agcagtgatg ctattttatc aaatcggcga gtatttccaa
    10681 ggattggcag tcagtcattc aagaaaatcg attcgcgaat tgatggcgat ccgtccagaa
    10741 acagctcacg tgcagacgac ggaaggattg atgaccgtga accctgaaga cgtgcagatc
    10801 ggtcagtttg tacttgtgaa gcctggtgaa cgtgtaccgt tagatggaac gatcatcgaa
    10861 ggagaatcat tagtcgatac gtcagcactt acaggagaat cagtccctaa atctgtatat
    10921 gttggtgaaa cagtattaag tggatttatc aataaaaata aaccattttt agttcaagta
    10981 gaaaaatctt atgaaaattc tactatcagc aaattattag aattagtaga aaatgccagc
    11041 agtaaaaaag caccagctga aaatttcatt actaaattcg cgcgctatta cacaccagtc
    11101 gttgtaggat tggccgtttt attagctgtt ttacctccac ttgttgtttc tggagcggcg
    11161 ttttctgaat ggatctatcg agcattgaca tttttagtga tctcatgtcc atgtgcatta
    11221 gtgatctccg taccccttag tttctttggc ggtatcggag gagcaagtaa aatcggtgta
    11281 ttagtaaaag gttcaaatta cttggagttg cttgcacaaa ctgagacagt ggtattcgat
    11341 aaaaccggca cattaacgaa aggtgacttt tcgatccaga cgatcgatac aaaaatcgat
    11401 ccaaagatat tcatgcaata tgttgcgagt gcagaacagt tttctacaca tccgattgct
    11461 caatctgttt tagaaggcta cgcaggtccg cttttaccaa cagctaatat ccaagaattt
    11521 gcaggtgaag ggatcttagc tgaagttgat ggcaaacaag tattagcagg taatcacaaa
    11581 ttgatggaaa gatttgaaat ctcttttcct tcaagtcaag aaatcggtac attgctttat
    11641 ctagcgatcg atcaaagtta cagtggttat cttgttattg cagatacctt gaaagaagat
    11701 gccgtagacg cattggttca attaaaaaag gctggtgtga agaacacagt catgttgacg
    11761 ggcgattcga aaaaaattgc tgatcatatc gggaagcaag taggcgtgga caagatatat
    11821 agcgaattgc ttccagaaga taaagtgcag cggttagagg aaatcttaca aagaaacaat
    11881 aaaaagaccg catttgtcgg agatggaatc aacgatgcac cggtgctagc aagagcagat
    11941 gtaggaatcg caatgggtgg attgggaagc gatgctgcaa ttgaagcagc agatgtagtc
    12001 atcatgaatg atcaaccatc gaagattgca gaagcaatcc atttagcaaa gaaaacattg
    12061 aaaatcgtca aacagaacat tgtctttgct attgggataa aaattttggt acttgcctta
    12121 ggtgcttttg gttttgcatc aatgggagat gcagtatttg cagatgttgg ggtgacagta
    12181 ttagctgttt tgaatgccat gcgaagcttg catgtaaaaa ataaacgttg atcaatgaag
    12241 aggacgaatg aacgtcctct tttttttata tccttttctt aatgaaaaat gtaa
//
LOCUS       DAZKLD010000092        12240 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.092, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000092 DAZKLD010000000
VERSION     DAZKLD010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12240)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12240)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12240
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.092"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(131..688)
                     /gene="frr"
                     /locus_tag="ACG8KR_001989"
     CDS             complement(131..688)
                     /gene="frr"
                     /locus_tag="ACG8KR_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HFQ0201348.1"
                     /translation="MADAIMTEAKEKMKKAAQNLQRELGQIRAGRANASLLDRITVNY
                     YGAPTPLNQMASIQIPEARVLMITPFDKSILQDVEKAIMASDIGISPTNDGNVIRLVI
                     PQLTEERRKELAKDVKKEAENAKIAIRNIRRDAIDEYKKQQKNGDITEDDLRGLEKDV
                     QTLTDNSIKEVDKIAADKEKELLEV"
     gene            complement(691..1413)
                     /gene="pyrH"
                     /locus_tag="ACG8KR_001990"
     CDS             complement(691..1413)
                     /gene="pyrH"
                     /locus_tag="ACG8KR_001990"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HFQ0201349.1"
                     /translation="MVTPKYQRVVLKLSGEALAGDAGFGIKPPVIKEIIQEIKEVHEL
                     GVEMAIVVGGGNIWRGQIGAQMGMERAQADYMGMLATVMNALALQDTLENVGVPTRVQ
                     TSIEMRQIAEPYIRRKAERHLEKGRIVIFAGGTGNPYFSTDTTAALRAAEIGADVILM
                     AKNNVDGVYSADPKVDANAVKFEELTHLEVIAKGLQVMDSTASSLSMDNDIPLLVFNL
                     NEHGNIRRAILGENIGTTVRGK"
     gene            complement(1549..2430)
                     /gene="tsf"
                     /locus_tag="ACG8KR_001991"
     CDS             complement(1549..2430)
                     /gene="tsf"
                     /locus_tag="ACG8KR_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HFQ0201350.1"
                     /translation="MADVTAKMVKELREMTGVGMMDAKKALVEVEGDMEKAVDLLREK
                     GMAKAAKKNDRIAAEGLASVAVKGNTAAIVEVNSETDFVSKNEMFQDLVKEIAELVAE
                     NKPADMEAAMKIKTDKGTIESDLIEATQVIGEKISFRRFEVVEKDDNAAFGGYLHMGG
                     RIAVLTVLDGTTDETVARDVAMHVAAINPRYVNESQIPEAELEHEKTVLTEQALNEGK
                     PANIVEKMVEGRLKKFKAEIALVDQPFVKDPDMTVEKYVASKGATVKTFVRFEVGEGI
                     EKREDNFVEEVMSQVKK"
     gene            complement(2529..3311)
                     /gene="rpsB"
                     /locus_tag="ACG8KR_001992"
     CDS             complement(2529..3311)
                     /gene="rpsB"
                     /locus_tag="ACG8KR_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HFQ0201351.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKLVDAAYDYMKSVAEEGGVALFVGTKKQAQEAIKEEATRAGQYFVNHRWLGGTLT
                     NWDTIQKRISRLKQINAMEEDGTFEVLPKKEVAGLNKERERLEKFLGGIADMPRIPDV
                     MYIVDPRKERIAVQEAHKLNIPIVAMVDTNCDPDEIDVVIPSNDDAIRAVKLITSKMA
                     DAFIEGNQGEDQVVEEDFTEENTATSIEEIVDVVEGDNSSAE"
     gene            3670..4149
                     /locus_tag="ACG8KR_001993"
     CDS             3670..4149
                     /locus_tag="ACG8KR_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HFQ0201352.1"
                     /translation="MRKAQRHLLIKQVIEEFPIRTQEELLNKLGHYGVTATQATISRD
                     IRDLKIVKAPDENGVSRFVLFREKGTPETRTEEEDRLVQMIEDIVLKVERVHFLTIVN
                     TLPDNAHLFASVLDEIKPPSIVSTIAGFDTIMVISENEEDAKRVEEFLHHPTEVSLF"
     gene            4366..5457
                     /gene="phnW"
                     /locus_tag="ACG8KR_001994"
     CDS             4366..5457
                     /gene="phnW"
                     /locus_tag="ACG8KR_001994"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047304 - 2-aminoethylphosphonate-pyruvate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HFQ0201353.1"
                     /translation="MYKLLTPGPLTTSETVKNAMLIDHCTWDQEYKEITQWIRKKLLD
                     LAQVSEGIYTTVLMQGSGSFGVESVLSSVIRPEDTVLICSNGAYGQRMINMCECLKLN
                     YTVYEVSEHQILQAHVITQLIEENPSISALAMVHSETTSGILNNLEEIAAVTQEKDLL
                     FIVDAMSSFGGIPIRVAELGIDFLISSSNKCIQGVPGFSFVLANKAELEKRKNVSRSL
                     SLDLYDQYRVMENENGKWRFTSPTHTVLAFQQALKELEAEGGIETRYSRYLANNHVLR
                     QKMTEMGFQPFIQEYQGPFITSFLYPKDWRFDFNHFYQYLKQNGFVIYPGKISDHECF
                     RIGNIGEIYPEDILELTAIIKDYKENVND"
     gene            5450..6235
                     /gene="phnX"
                     /locus_tag="ACG8KR_001995"
     CDS             5450..6235
                     /gene="phnX"
                     /locus_tag="ACG8KR_001995"
                     /EC_number="3.11.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050194 - phosphonoacetaldehyde hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HFQ0201354.1"
                     /translation="MIKTVIFDWAGTTVDFGCMAPVHAFRNAFLEKGIQLTDKEIREP
                     MGKLKWDHIQQLLSLPSVKKQWIAIYGQLPQEKEVEELYQQFEHYLFKDLVKHSTLKP
                     DTLETIERLRNAGINIGSTTGFTAPMMTIVAETAKKEGYSPDFMATSEDVEGYGRPFP
                     YMIHKNMQYFKNKSVDEVIKVGDTVSDIQEGKNAGVLTVGVIEGSSLMGISYDEYQKL
                     EMGKKDRLKQSVALRFQQAGADICVNNLKELTEYILYADKNPE"
     gene            complement(6581..8272)
                     /gene="argS"
                     /locus_tag="ACG8KR_001996"
     CDS             complement(6581..8272)
                     /gene="argS"
                     /locus_tag="ACG8KR_001996"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HFQ0201355.1"
                     /translation="MNNKDLVAKAVYDAVKDDLTLEQVEQLLENPKSAEHGDVAFPAF
                     SLAKVYRKAPQQIAADLAEKIDSANFEKIEVVGPYLNFFMNKELISKKVLQTVVKEKE
                     HYGDSNIGNQGTVPIDMSSPNIAKPISMGHLRSTVIGNSIGFIMEKIGYQPIRINHLG
                     DWGTQFGKLIVAYKKWGTEEAVKAEPINELLRLYVQFHEVAETEPELNEEARAWFKRL
                     EEGDEEAIQLWQWFRDESMKEFNKIYDLLEVRFDSLNGEAFYNDKMDEIVKLLEEKHL
                     LNEDKGAEIVDLSAYDLNPALIKKSDGATLYITRDLAAALYRKRTYDFKQSLYVVGNE
                     QSYHFKQLKAVLKEMGFDWSDDMRHIPFGLITQGGKKLSTRKGKIVLLEEVLNEAIQS
                     AKEQISEKNPDLENKDAVAKQVGVGAVIFHDLKNDRLNTFDFNLEEVVRFEGETGPYV
                     QYTHARAVSLLEKAGFVPSETADYALNDDTSWEVVKLVQKYPETVLSAGEKYEPSVIA
                     KHAIKLAQAFNKYYAHTKILADDEQKEARLALVYAVTVLLKEDLRLLGLHAPDKM"
     gene            complement(8694..9641)
                     /gene="arcC"
                     /locus_tag="ACG8KR_001997"
     CDS             complement(8694..9641)
                     /gene="arcC"
                     /locus_tag="ACG8KR_001997"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HFQ0201356.1"
                     /translation="MAKRKVVVALGGNAILSTDASAKAQQEALMETAKYLVKFIEQGD
                     ELIISHGNGPQVGNLLIQQQAADSEKTPAMPLDTCVAMTEGSIGYWLQNAMGEVLKEK
                     GIDKDVVSLVTQVIVDENDPSFKNPSKPVGPFYTEEEAKEQMNADSTVTFKEDAGRGW
                     RKVVASPKPISIKEARVIETLVDQGVITVSVGGGGIPVVETATGLEGREAVIDKDFAS
                     EKLAEIIDADLLIVLTGVDNVYVNYQKPDQKKLETVTVSEMKQYIDEKQFAPGSMLPK
                     VEAAIAFVEAKPNAKAIITSLENIENLLASEEGTIIVAD"
     gene            complement(9756..10775)
                     /gene="argF"
                     /locus_tag="ACG8KR_001998"
     CDS             complement(9756..10775)
                     /gene="argF"
                     /locus_tag="ACG8KR_001998"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HFQ0201357.1"
                     /translation="MKESVFQGRSLLAEKDFTKEELQYLIDFSEHLKDLKKRGIPHHY
                     LEGKNIALLFEKTSTRTRSAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQKMVEELAEFSGVPVWNGLTDEWHPTQMIADFLTIQENFGTVEGITVA
                     YCGDGRNNMANSLLVTGAILGANMRIVAPKELQPEEEIVKMAEGFAEKSGAQLMITDD
                     VDKGVDGADVLYSDVWVSMGEEDKFEERIKLLKPYQINMEMVEKTHNTDRLIFLHCLP
                     AFHDTNTVYGEQMKERFGITEMEVTDEVFRSKYARQFDQAENRMHSIKAIMAATLGNL
                     FIPRV"
     gene            complement(10866..12095)
                     /gene="arcA"
                     /locus_tag="ACG8KR_001999"
     CDS             complement(10866..12095)
                     /gene="arcA"
                     /locus_tag="ACG8KR_001999"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016990 - arginine deiminase activity
                     [Evidence IEA];
                     GO_process: GO:0019546 - arginine deiminase pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HFQ0201358.1"
                     /translation="MDKPIHVFSEIGKLKTVLLKRPGQEVENLTPDIMDRLLFDDIPY
                     LPIAQEEHDNFAKTLQNEGVETLYLEKLAAEAIDAGNVKEQFLNKMLDESHIASNAVR
                     EGLHEFLLSMETQEMVDKIMAGVRTKDIEVRSSSLYDLSADDDYPFYMDPMPNLYFTR
                     DPSASMGNGMTVNKMTFEARRRESMFTEYILKHHPRFANKGIEVWLDRENPDHIEGGD
                     ELILSDKVVAVGISQRTNAKALEKLARHLFAKNSGFEKVLAIKIPNNRAMMHLDTVFT
                     MVDHDKFTIHPAIQSKDGKMDVFTIEPDGDDIKITHSDNLHETMKAALGLDDLVLIPT
                     GNGDEIVAPREQWNDGSNTLAIAPGVVVTYNRNYVSNELLRSYGIKVLEINSSELSRG
                     RGGPRCMSQPLVREDLK"
BASE COUNT         3361 a         2540 c         2194 g         4145 t
ORIGIN      
        1 tttcctatga aatcattgtt tttttattta aaaactgaat aattttttct ttttttaatt
       61 tttaaataaa agaaatataa ataaaaacag aggaagcgga atgatccgcc cctctgtttt
      121 tatgatttta ttaaacttcc aataattctt tttctttatc agcagcgatc ttatctactt
      181 ctttaatgct gttatctgtc aatgtttgta cgtctttttc taatccgcgc aaatcatctt
      241 cggtaatatc accatttttt tgttgttttt tatattcatc gatcgcatca cgacggatgt
      301 tacgaatcgc gatttttgca ttttctgctt cttttttcac atctttagct aattcttttc
      361 tgcgttcttc tgtcagttgc gggataacta agcgaatgac atttccatca ttggttggac
      421 tgattccgat atcacttgcc ataatagctt tttcaacatc ttgaagaatg ctcttgtcaa
      481 aaggtgtgat cattaaaaca cgtgcttctg gaatttggat tgatgccatt tggttcaatg
      541 gtgttggcgc accgtagtag ttcacagtaa tacggtccaa aaggctagcg ttcgcacggc
      601 ctgcgcggat ttgcccaagt tcacgttgaa gattttgtgc tgcttttttc attttttctt
      661 ttgcttctgt cataattgca tctgccattt ttatttcccc cttacagttg ttccgatatt
      721 ttcgcctaag attgcacggc gaatatttcc gtgttcattt aaattgaaga caagcagtgg
      781 aatatcatta tccatgctta gtgaacttgc agttgaatcc atgacttgta atcctttggc
      841 aataacttcc agatgagtca gttcttcaaa ttttactgca tttgcatcta ctttcggatc
      901 agcagaataa acaccgtcta cattgttttt agccatcagg ataacatctg cgccgatttc
      961 tgccgctcgt aatgctgctg ttgtgtctgt cgagaagtac gggttgccgg taccgcctgc
     1021 aaagataacg atccgtcctt tttctaaatg acgttctgct ttacggcgaa tatatggctc
     1081 tgcgatttga cgcatttcaa tcgaagtttg cacgcgtgtc ggtacaccca cgttttccaa
     1141 cgtatcctgt aaagctaagg cattcataac tgtagctaac atacccatgt aatcagcttg
     1201 agcacgttcc atgcccattt gtgctccgat ttgtccgcgc cagatatttc cgccacccac
     1261 aacgattgcc atttcaacgc ccagctcgtg aacttctttg atttcttgga tgatttcttt
     1321 gattactggt ggtttgattc caaatcctgc atccccagct aaagcttctc cactcaattt
     1381 taagactaca cgctgatatt tcggtgtgac catgttcatt cctccaatat tttctattgc
     1441 cattttacca tacttagatg atttcgacac tctaagactg gtttttcttg ttttgcttaa
     1501 aaaaagggag cgcacaaacc gtgcgttccc gtctgttatt caacagaatt attttttcac
     1561 ttggctcata acttcttcta caaagttatc ttcgcgtttt tcgatacctt cgccaacttc
     1621 gaaacgtacg aatgttttaa cagttgcgcc ttttgatgca acgtattttt caacagtcat
     1681 atcaggatct ttaacgaatg gttggtccac caaagcgatt tctgctttga atttcttcaa
     1741 gcgtccttct accatttttt caacgatatt cgctggtttg ccttcgttca atgcttgttc
     1801 tgtaagaaca gttttttcat gttctaattc agcttctgga atttgtgatt cgtttacata
     1861 acgaggattg atagctgcta cgtgcatagc tacgtctctt gcaactgttt cgtcagttgt
     1921 tccatctaga acagtcaata cggcaatacg tccgcccata tgcaagtatc caccaaatgc
     1981 agcattgtcg tctttttcta caacttcaaa gcgacggaag ctgatttttt ctccgataac
     2041 ttgagttgct tcgatcaagt cagattcaat tgtaccttta tctgttttga ttttcattgc
     2101 agcttccatg tctgctggtt tgttttcagc aacaagttct gcaatttctt ttactaaatc
     2161 ttggaacatt tcgtttttag aaacaaagtc tgtttctgag tttacttcaa cgattgctgc
     2221 agtgttacct ttcacagcaa ctgaagccaa tccttcagct gcaatgcggt catttttctt
     2281 cgcagccttt gccattcctt tttcacgtaa aagatcaact gctttttcca tgtcgccttc
     2341 tacttcaact aatgcttttt tcgcatccat cattcctacg ccagtcattt cgcgtagttc
     2401 ttttaccatt ttagctgtaa cgtctgccat gtttaactcc tcctgtgtgt ttttcttttt
     2461 aaaaaaagct gcttcaaagg agagtgaggc ttgtcgccca gcctttgaaa cagctccatc
     2521 attcaaaatt attctgctga agaattgtcg ccttcaacaa catctacgat ttcttcgatt
     2581 gatgttgctg tgttttcttc tgtgaaatct tcttcaacta cttgatcttc accttggttt
     2641 ccttcgatga aagcatcagc catttttgaa gtaatcaatt taacggcacg aattgcatcg
     2701 tcgtttgaag ggattactac gtcgatttca tctggatcgc agtttgtatc aaccatagct
     2761 acgattggga tatttaattt gtgtgcttct tgaacagcaa tacgttcttt acgtgggtca
     2821 acgatgtaca ttacatctgg gattcttggc atatcagcga taccgcctaa gaatttttca
     2881 agacgttcac gttctttgtt caaaccagca acttcttttt taggtagaac ttcaaaagtt
     2941 ccatcttctt ccattgcatt gatttgtttc aaacggctga tacgtttttg gatagtatcc
     3001 cagtttgtca atgttccgcc taaccaacga tggttaacga agtattgtcc tgcacgagtt
     3061 gcttcttctt tgattgcttc ttgtgcttgt ttttttgtac ctacgaataa tgctacgccg
     3121 ccttcttcag caacgctctt catgtaatca taagctgcat ctactaattt aactgttttt
     3181 tgcaagtcaa tgatgtagat tccgtttctt tctgtgaaga tatatttctt catttttgga
     3241 ttccagcgac gagtttggtg accaaagtgt acgccggctt ctagtaattg tttcatagaa
     3301 attactgcca tgtttgtttc ctccgatttg gttttatttt tcctcttctt atcttctacc
     3361 ttgccggcaa actttttcaa gcacctaccg acaatccaat aagaatgtgg atttgctgat
     3421 gcataaagca ccctcagcta ttatacagac aaaagatccg tatcgcaaga gcaaatatac
     3481 ttttctgctc aacattcaat agtttacctg aaagataaaa agaattcaag aaaaacagta
     3541 gatttctctt tgtttcttta ttatcctttt tccataaaat aattttaggt ataaaagacg
     3601 aatattattg ggcaatcctt cttttttttg ttatgataca tgtgaaatct ttcgtaaagg
     3661 agaaagaaaa tgcgcaaagc tcaaagacat ttgctgatta aacaagtcat tgaagaattc
     3721 ccgatccgta cgcaagaaga actattaaac aaacttgggc attatggcgt taccgccacg
     3781 caggcaacga tttctcgaga tatccgtgat ctaaaaatcg tgaaggcacc tgatgaaaat
     3841 ggcgtttccc gcttcgtatt gtttcgcgaa aaaggaactc ctgagactag gactgaggaa
     3901 gaggatcggc tggttcaaat gattgaagac attgtattga aagtcgaacg agtccacttt
     3961 ttgacgatcg tcaatacctt gccagacaat gctcatttat ttgcatctgt tttagatgag
     4021 atcaaacctc cctctattgt cagtacaatt gctggattcg atacgatcat ggtcatctcg
     4081 gaaaatgagg aggacgcaaa acgagtagaa gaatttcttc accatccaac tgaagtatca
     4141 cttttttaat cagagcacgt attcctaata tggaatgcgt gtttttttat tttcaatatt
     4201 ttccttatta aataaacctt tacactcttt acaaaaatac atcatttttt attcaatttt
     4261 ttcaacacct tgattgaatc acaataatca tcatttatat tggatgttaa caagagcagc
     4321 ttcttgctct ttgtgtattt caaaaaagaa atgaaaggaa atcatatgta taaattatta
     4381 actcctggac cattgacgac ttctgaaaca gtgaaaaatg ctatgctgat cgatcattgt
     4441 acttgggatc aagaatacaa agaaattaca caatggataa gaaaaaaatt attggatctt
     4501 gctcaagtat cggaaggaat atacacaacg gtcttaatgc aaggtagcgg ctcctttggg
     4561 gtagaatcgg tattaagttc tgtgatcagg ccagaagata ctgtactgat ttgttcaaac
     4621 ggcgcatatg gacaacgaat gatcaacatg tgcgaatgtc tgaaactgaa ctataccgtt
     4681 tatgaggtat cggaacatca aatcctccaa gctcatgtga ttacacagct gattgaagag
     4741 aatccctcta tctccgcact agcaatggtc catagcgaga ctacttccgg tattttgaat
     4801 aatttggaag agattgctgc tgtcacacaa gaaaaagatc ttttgtttat agtggacgcg
     4861 atgtcaagct ttggtgggat accgattcgt gtagccgaac tagggattga ttttttgatt
     4921 tccagctcaa acaaatgtat tcaaggtgtt ccaggctttt cttttgtact tgctaataaa
     4981 gcggaactag aaaagcgaaa aaatgtgagt cgaagtttgt cacttgatct atatgaccag
     5041 taccgcgtga tggaaaacga gaatggcaaa tggcgtttca cttcccctac ccatacggtt
     5101 ttggcttttc aacaagcgct gaaggaacta gaggcagaag gaggaatcga aactcgatat
     5161 tcacgttatc tcgccaataa tcatgtacta cgacaaaaaa tgacagagat ggggttccag
     5221 cctttcattc aagaatatca ggggccattc atcacctctt ttctttaccc taaggactgg
     5281 cgatttgatt ttaatcattt ttatcagtat ttaaagcaaa acggatttgt gatctatccc
     5341 gggaaaatca gcgatcatga atgttttcgt atcggaaata ttggcgagat ctatccggaa
     5401 gatattttgg aactgacagc aattatcaaa gactataagg agaacgttaa tgattaaaac
     5461 tgtaattttt gattgggcag gaacaactgt ggactttggc tgtatggctc ctgtccacgc
     5521 ttttagaaat gcttttctag aaaaaggaat ccaactgact gacaaagaaa tccgcgaacc
     5581 gatgggaaaa ttaaaatggg atcatatcca acagcttctt tccctccctt ctgtcaaaaa
     5641 acaatggata gctatttatg gacaactacc tcaggaaaaa gaggtggagg aattgtatca
     5701 acaatttgaa cattatctgt ttaaagattt ggtcaagcat tctaccttga aacccgacac
     5761 attggaaacg atcgaacggt tgagaaacgc tgggatcaat attggttcca cgactggatt
     5821 tactgctcct atgatgacaa tcgtagcaga aaccgcaaaa aaagaagggt attcaccaga
     5881 cttcatggca acttctgaag acgtcgaagg gtatggtcga ccgtttcctt atatgatcca
     5941 taaaaacatg cagtacttca aaaacaagtc ggttgatgaa gtaatcaaag tcggagatac
     6001 ggtctcagat atccaggaag ggaaaaatgc cggagtgttg acagtaggtg taattgaagg
     6061 tagttcgcta atggggattt cttatgatga gtaccaaaaa ttagagatgg ggaaaaaaga
     6121 tcgtttaaaa cagtctgttg ctcttagatt ccaacaagct ggcgctgata tttgtgtaaa
     6181 caatttaaaa gagttaacag aatacatttt gtacgctgac aagaatccag aatgactttt
     6241 atacaaaatc agaggtcgtg aaaaagctgt actgcatcaa gtaataagaa cagccaaaca
     6301 aaaatagctc ctcatatttt tcgtttggtt aggcttatta ccgcagatgc aagcttttga
     6361 acaccgttta ttcgaaaaaa aaagggagct gtgacacgac ttttgtcaca gtccccttat
     6421 ttacaaacaa acagtgttta agagacaacc tctccactaa acggtgttca ttcagctgac
     6481 tctcggtatt tgctcaaaaa acgataaaat ttgaaaagct attttctctt ttgtttctct
     6541 aatactcgga tcagaacgct gatttcacat tctctttttc ttacatctta tctggtgcat
     6601 gaagtcctag caaacgaagg tcttctttca ataatacagt cactgcataa actagagcta
     6661 agcgtgcttc tttttgttca tcatcagcta aaatcttcgt atgagcatag tatttgttga
     6721 atgcttgtgc taatttgatt gcatgtttcg caatcactga cggttcgtat ttttccccag
     6781 cagacaagac cgtttcaggg tatttttgaa ctaatttaac cacttcccag ctggtatcgt
     6841 cattcaatgc atagtctgct gtttctgaag ggacaaatcc tgctttttcc aacaggctga
     6901 ctgcacgagc atgtgtatat tgcacataag gtccagtttc tccttcaaat cgaacaactt
     6961 cttccaggtt aaagtcaaat gtattcagtc gatcgttttt caaatcgtgg aaaatgactg
     7021 ccccaacccc aacttgttta gctacagcat ctttgttttc caaatcaggg tttttctcgc
     7081 tgatttgttc tttagcagat tggattgctt catttaatac ttcttctaat aagacgatct
     7141 ttcctttacg agttgataat tttttaccac cttgcgtgat caaaccaaat ggaatatggc
     7201 gcatatcgtc agaccagtca aagcccattt ccttcaagac agctttcaac tgtttaaaat
     7261 gatagctttg ttcattccct actacatata gagattgttt gaagtcgtaa gtacgtttac
     7321 ggtacaatgc agcagctaag tcacgtgtaa tgtatagtgt cgcaccgtct gacttcttaa
     7381 tcaacgctgg attcaggtca taagcagata ggtcaacgat ctctgcacct ttatcctcgt
     7441 tcagcaaatg tttttcttct aaaagtttga cgatttcatc catcttgtca ttgtagaaag
     7501 cttcaccgtt caacgaatcg aaacgtactt ccagcagatc atagatttta ttgaattctt
     7561 tcatcgattc atcacggaac cattgccata attgaatagc ctcttcatcg ccttcttcca
     7621 gacgtttgaa ccaagcacga gcttcttcat ttaattctgg ttctgtttca gctacttcat
     7681 ggaattggac atataaacga agcaattcat tgattggttc tgctttaaca gcttcttctg
     7741 taccccattt tttgtaagcg acgatcagtt ttccaaactg tgttccccag tcacctaaat
     7801 ggttgatgcg gattggctga taaccgattt tttccataat gaaaccgatc gaattaccga
     7861 taactgtcga acgcaaatgt cccattgaga ttggtttggc aatattagga gaagacatgt
     7921 cgattggtac tgttccttga ttcccgatat tgctatcccc ataatgttct ttttctttca
     7981 ctactgtttg caaaactttt ttgctgataa gttctttatt catgaaaaag ttcaagtaag
     8041 gtccgacaac ttcgattttt tcaaagttgg cagaatcgat tttttctgct aaatctgcag
     8101 cgatttgctg tggcgctttt cggtaaactt ttgctaatga aaaggctgga aatgcaacat
     8161 ctccgtgttc agctgatttt ggattttcta gtaattgttc gacttgttcc agtgttaaat
     8221 catctttgac agcgtcatag acagcttttg cgacaagatc tttattattc atctttattc
     8281 ctcctaaagt gctcccttga ttgttaaaca aaaaaaatct ctagcgacgg catacttagt
     8341 atgccatcac tagagacgag atttcccgcg gtaccactcc aattgtccta aaaaaggacc
     8401 agctccatct gatatcggta gacgaccgtc tgtttaaaac agaattctcc aagtgcgctt
     8461 cgttgtatct tcttcattcg gcttccacca cttccgaatt cgctagccaa gaatgataca
     8521 agtactctct tgttcaacaa atcttagtct tattaaactt tcttgaatta tatcagaaaa
     8581 aaaaacgaaa ggctagtcgt ttctgataaa aaaacaatcc agatggccta aaggagaaga
     8641 aataccacct ggatagctat aaatcatttc gtaaatacga aagacttaac aaattaatca
     8701 gctacgataa tcgttccttc ttcagaagct aaaagattct cgatattttc taaagaagtg
     8761 atgattgcct tagcgtttgg ttttgcttca acaaatgcaa tagctgcctc tactttcgga
     8821 agcatgcttc ctggagcaaa ttgcttttca tcaatgtatt gtttcatttc tgaaacagta
     8881 acagtttcta atttcttttg atctggtttt tggtaattga catacacatt gtctactcct
     8941 gtcaatacga tcagcaaatc cgcatcgatg atttcggcta atttttctga agcaaaatct
     9001 ttatcgatta cggcttctcg tccttctaat ccagtagctg tttctacaac aggaatccca
     9061 ccgccaccaa cagagactgt gataactcct tgatcgacca gcgtttcgat gacacgtgct
     9121 tctttaatcg agatgggttt aggggaagcg actacttttc tccatccacg tccagcatct
     9181 tctttgaatg tcacagtgga gtcagcattc atttgttctt tggcttcttc ttccgtgtag
     9241 aatggaccga ctggtttact tggatttttg aatgaaggat cattttcatc cacaataact
     9301 tgagtgacta aagaaaccac atctttatcg atcccttttt ccttcagtac ttcgcccatg
     9361 gcattttgta accagtaacc gatagatcct tctgtcattg caacacatgt atctaaaggc
     9421 attgccggtg ttttctcaga atcagcagct tgctgctgaa tcaataggtt accgacttgc
     9481 ggaccatttc cgtgggagat gatcaattca tctccttgtt cgataaattt cactaaatac
     9541 tttgctgttt ccattaacgc ttcttgttgg gcttttgcac ttgcatccgt agacaagatt
     9601 gcattacccc ctaaagcgac aactactttt cgtttggcca tttcattcgt cctttcttca
     9661 gttctaccga attttctaga taacaaaacg agccagcgtt ttgtgatttt ccctcaatgc
     9721 tgactcgttt tttcgtgaga tgaatgtggt tatgcttaaa cgcgagggat aaacaagtta
     9781 cctaatgttg ctgccatgat cgctttgatt gagtgcatac gattttctgc ttggtcaaat
     9841 tgacgagcat atttgctgcg gaagacttca tctgttactt ccatttcagt gatgccaaag
     9901 cgttctttca tttgttcacc gtaaacagtg tttgtatcat ggaaagctgg caagcagtgc
     9961 aagaagatca aacgatctgt gttgtgtgtt ttttctacca tttccatatt gatttggtaa
    10021 ggtttcaaca atttgatacg ttcttcgaat ttatcttctt cacccattga tacccataca
    10081 tcagaataaa ggacgtctgc tccgtctaca cctttatcaa catcgtcggt aatcatcaat
    10141 tgtgcaccgg atttttcagc aaagccttct gccattttga cgatttcttc ttcaggttgt
    10201 aattcttttg gtgcaacgat acgcatattc gctcctaaaa ttgcacctgt gactaataaa
    10261 gagttagcca tattgtttcg accatctccg cagtaagcaa cagtgatacc ttctactgtt
    10321 ccaaagtttt cttggatagt taaaaagtca gcgatcattt gtgttggatg ccattcatca
    10381 gtcaagccat tccaaactgg aacaccagag aattctgcta gttcctctac cattttttga
    10441 ctgaatccac ggaattcgat tccatcaaac atacgaccta gtactttagc tgtatcttct
    10501 gtggattctt ttttacctaa ttggatatca tttgcaccta gatattcagg atgtgcacct
    10561 agatcgatcg ctgcagtagt aaatgcagaa cgagtacgtg tagatgtttt ttcaaataaa
    10621 agcgcaatat tttttccttc tagatagtga tgtgggatac cacgtttttt caagtctttc
    10681 aagtgttctg aaaaatcgat caaatattgt aattcttctt ttgtaaagtc tttttctgct
    10741 aataagcttc ttccttggaa tactgattct ttcatattaa acattctcct ttagattgtt
    10801 tttatttaag attaaagagg aaatcccgac ggcagctgac agtaagaccg ccgtcaggaa
    10861 acaaattact tcaagtcttc acggacaagt ggttggctca tgcagcgtgg accaccacgg
    10921 cctcgtgaca attcacttga attgatttct aacactttga tgccatagct acgaagcaat
    10981 tcgttcgata cataatttcg gttataagtg actacaacac ctggagcgat tgccaatgtg
    11041 tttgatccat cattccattg ttctcgaggt gcaacgatct catctccatt accagttggg
    11101 atcagtacaa gatcatctaa tccaagagca gctttcatcg tttcgtgtaa gttatctgaa
    11161 tgagtaatct tgatatcatc accatctggt tcgattgtaa agacatccat cttaccgtct
    11221 ttactttgaa ttgctggatg gatagtgaat ttatcgtgat ctaccattgt aaatacggta
    11281 tccaaatgca tcattgcacg gttgtttgga attttgattg ctaacacttt ttcaaatcct
    11341 gaattttttg cgaataggtg gcgtgctaat ttttctaaag ctttcgcatt agttcgttga
    11401 gaaatcccaa cagctactac tttatcgctt aagatcaatt cgtctccgcc ttcgatatga
    11461 tctggatttt cacgatccaa ccatacttcg attcctttat ttgcaaatct tggatgatgt
    11521 ttcaaaatat attctgtaaa catcgattca cgacgacgtg cttcaaaagt catcttattg
    11581 actgtcatcc catttcccat tgatgcggaa gggtcgcgtg taaagtacaa gtttggcatt
    11641 gggtccatgt aaaatggata gtcatcgtct gctgacagat cgtatagact gctagaacgt
    11701 acttcaatat cttttgtacg aaccccagcc atgattttat ctaccatttc ttgcgtttcc
    11761 attgatagaa ggaactcatg aagaccctct cttacagcat tcgatgcaat atgagattca
    11821 tccaacattt tattcaagaa ttgttctttg acattgcctg cgtcaatcgc ttctgccgct
    11881 aatttttcca agtaaagagt ctcgacgcct tcattttgca atgtctttgc aaagttgtca
    11941 tgttcttctt gagcaatcgg aagatatgga atatcatcga acaacagacg atccataata
    12001 tctggtgtca aattttccac ttcttgtcca ggccttttca gtaaaactgt ctttaatttc
    12061 cctatttcag agaaaacgtg aataggttta tccatgttgt gttgcctcct tctttttgat
    12121 tacatgtata ggatatcctc cttttgtaat cactttcata attttgtctc attcaaaatg
    12181 agacattcct cacattaata ttcgataatt aaataattaa acgtttttct atttttaagc
//
LOCUS       DAZKLD010000093        12182 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.093, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000093 DAZKLD010000000
VERSION     DAZKLD010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12182)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12182)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12182
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.093"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(51..1517)
                     /locus_tag="ACG8KR_002000"
     CDS             complement(51..1517)
                     /locus_tag="ACG8KR_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HFQ0201359.1"
                     /translation="MEKQQDKGFFGQPKGLSTLFFTEMWERFSYYGMRALLIYYMYDT
                     VANGGLGLPRATALAIMSIYGSLIYMSSIVGGWVSDRVLGAKRTVFFGGVFIMLGHIV
                     LALPFGVSTLFVSMALIIIGTAFLKPNVSGMVGHLYSKTDLRRDAGFSIFYMGVNLGA
                     LLAPLIVGTIGQEYNYHLGFSLAAIGMFFGLLQYIIQGRKTLDGVGTTPPNPLTTEER
                     KGFIRNLIIALVVIAAVFGGAQLTGHLTIDFFVNTISVLGIILPLYYFIKMLTSKDVT
                     AEEKPRVWAYIPLFLAAIIFWSLQEQGSSILALFASERTQSTLFGVTIQASWYQSLNP
                     IFIFILTPVFVTMWTKLGERQPSTVVKFSLGLLFAGLSYLLLMLPGMLYGTSGKVSPL
                     WLLGSFFIVVIAELCLSPVGLSVTTKLAPRAFESQTIAIWFLADASSQAINAQIARFY
                     TPGTESAYFGIIGAVAIVGGILLFTVKEPIKKLMGSIH"
     gene            1904..2500
                     /locus_tag="ACG8KR_002001"
     CDS             1904..2500
                     /locus_tag="ACG8KR_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFQ0201360.1"
                     /translation="MTTFTDTLKNRRSIYHLGRNVSLSNEELTTLIKEAIKESPTAFN
                     AQSTRAVILFGDAHEKLWEITEEALRPLTPAEAFPNTQNKLAGFKNGYGTVLFFKDTD
                     VVKGLQEQFELYADNFPDWSEQSNGIATANTWVALVDKGLGANLQHYNPVIDEAVAKE
                     WNIPSNWKLRSQLVFGSPETPAGEKEYMNDADRFRVFG"
     gene            complement(2558..3040)
                     /locus_tag="ACG8KR_002002"
     CDS             complement(2558..3040)
                     /locus_tag="ACG8KR_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0201361.1"
                     /translation="MVTFFTVLLFLFVVVMFLFLWLVRKEIIYRTVRNRWVYLVIPFL
                     VSLVIWYTLISHPTADELAKGILSAMIFISFLLDSRGITEEGLVLNSFDKKGVPFSEI
                     NKIVLYQPKGSKIVKMNFFRNGWRGPMLKFSASLEELVPFLSQRLNDEAEIDIMIDPE
                     "
     gene            complement(3146..3343)
                     /locus_tag="ACG8KR_002003"
     CDS             complement(3146..3343)
                     /locus_tag="ACG8KR_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0201362.1"
                     /translation="MTEEMINLGEQYACKPIGFTKTVIGEVVSKMTNCAVVKVAQCAA
                     EDQELLDEKASMVVAKYDTFE"
     gene            3638..4396
                     /locus_tag="ACG8KR_002004"
     CDS             3638..4396
                     /locus_tag="ACG8KR_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HFQ0201363.1"
                     /translation="MKKKTFKKLLFLGGTAALLLSGTSYYLHIHSPEHTMNTAQKSST
                     QKTKKTKEKAEVLLDVPLESQFDSPSLENGCEVTSLSMLLSFYGYETTKNQLAEQLDY
                     VPVFNADGTHGDPNEGFVGDISGGDWAMVVYVPPVASLAQQIVQTDYHTFPKTDAQLD
                     DIKKALSKGNPVWTSVTINFEVPDETDFMTWTTNNGEVRVTPLVHACVVTGYDKENIY
                     VNDPYGVKNRAVPINDFSAIFTAMGGQMLTLEKS"
     gene            4456..4884
                     /locus_tag="ACG8KR_002005"
     CDS             4456..4884
                     /locus_tag="ACG8KR_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0201364.1"
                     /translation="MEKFTHKKMDPNEIPIIFVRDCKGNVQGKVSINEWNERRRPATL
                     NELEIKLYRQSLVYYADQEYEKATDLLKFLIARTEYTRFEYIERLANIYHIMNEPVKE
                     YQLLDTVLSVAELIALPAGLEKKLVRRLLRVKQQLSDQEK"
     gene            4930..5002
                     /locus_tag="ACG8KR_002006"
     tRNA            4930..5002
                     /locus_tag="ACG8KR_002006"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4963..4965,aa:Gln,seq:ctg)
     gene            complement(5152..5523)
                     /locus_tag="ACG8KR_002007"
     CDS             complement(5152..5523)
                     /locus_tag="ACG8KR_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFQ0201365.1"
                     /translation="MKVIKYLIGLAAISGILLFGLKYYTEGSYGEIPGILDQLNPLVE
                     KGEVYVKTQKPEEVNEYGTARYVQKAADANGKERTVEFNGLSVLKENHYLKISNKGAH
                     IETYEEVPREKVPEKALAIIG"
     gene            5738..5923
                     /locus_tag="ACG8KR_002008"
     CDS             5738..5923
                     /locus_tag="ACG8KR_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0201366.1"
                     /translation="MNQEELFQKVKEMIKNGNFDGAKRFIEEHKEQLGPYKEKAQNLL
                     KDVNIDSVKNKFKNLFK"
     gene            complement(6006..6194)
                     /locus_tag="ACG8KR_002009"
     CDS             complement(6006..6194)
                     /locus_tag="ACG8KR_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0201367.1"
                     /translation="MNPYEQLIQDLIDGKIEKIDVKREELMAFREAWLKLENRKYVVG
                     KASLNGNVTYHYDPTRLF"
     gene            complement(6218..6481)
                     /locus_tag="ACG8KR_002010"
     CDS             complement(6218..6481)
                     /locus_tag="ACG8KR_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0201368.1"
                     /translation="MDHVVLEIDLHLINNIRVIYYVVSNSVEQRVLTNKINGILAKKD
                     VHRFNNGEGSYYSIPVEKIIYTTVKVREDLDTKKAYEPIFTTY"
     gene            6639..6896
                     /locus_tag="ACG8KR_002011"
     CDS             6639..6896
                     /locus_tag="ACG8KR_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HFQ0201369.1"
                     /translation="MIAIGQFVFYIPFFIMISILFYYIKWTKKKFSVLLASLPAVYFT
                     YQIFSFRHWETTSVLITHIIELTLSVIFLIIWIYFLYKNQN"
     gene            complement(6967..7158)
                     /locus_tag="ACG8KR_002012"
     CDS             complement(6967..7158)
                     /locus_tag="ACG8KR_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0201370.1"
                     /translation="MEAGKVLQEVKALCADKKYEEAKSLIEKNKENLEEKYPIAQEFI
                     ELKQGTILHRFKHFFGITE"
     gene            complement(7299..7619)
                     /locus_tag="ACG8KR_002013"
     CDS             complement(7299..7619)
                     /locus_tag="ACG8KR_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0201371.1"
                     /translation="MVRPQEVRAPKEKIEILAIIEDGTQTKKGYSIALVKWKGKKGVA
                     LRWDGDNQQDKGFPVTANGYHPAWFVLPDKFMELYSYDYARAAASIKALDKLTEEQNE
                     MEEK"
     gene            complement(7642..8076)
                     /locus_tag="ACG8KR_002014"
     CDS             complement(7642..8076)
                     /locus_tag="ACG8KR_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFQ0201372.1"
                     /translation="MSKRYQNIMVAVDGSRQSIKAFAEALDLAKDNEAHLFIVSIINK
                     VELTHSAYAFSKIYADEKQRTEVAMLKKINDAKEYGIAEIHAIVETGDPRNLIANVIP
                     GQENIDLIVMGATGKGAIQQALVGSTASYVVSHAPCSVLVVK"
     gene            8411..9049
                     /locus_tag="ACG8KR_002015"
     CDS             8411..9049
                     /locus_tag="ACG8KR_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFQ0201373.1"
                     /translation="MTSLKTLLFGTTLAAGAAFFMGTTAHADEAYTVQSGDTLSTISQ
                     KYVGDNSLINAIAESNSISDINLIYSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEPV
                     QAEQPVVQETVQTETQAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTAT
                     NGRYIGRYQLDSSYLNGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
     gene            complement(9373..9456)
                     /locus_tag="ACG8KR_002016"
     tRNA            complement(9373..9456)
                     /locus_tag="ACG8KR_002016"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9420..9422),aa:Leu,seq:cag)
     gene            9984..10484
                     /locus_tag="ACG8KR_002017"
     CDS             9984..10484
                     /locus_tag="ACG8KR_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0201374.1"
                     /translation="MKLIKLWEADLKKAYQLQTSFGPNESGFINNAYGYTFAEFLDYV
                     KKCEKTSLGIDLPESYVPATVFILESDEHEYVGIFNLRHKLNETLRNGAGHIGYGISP
                     NYRKKGYATQGLALVLQEAKKIGIDEALLSVNKKNLGSLKAQQKNGAAIYREDEKEYY
                     TKIKLM"
     gene            complement(10674..11018)
                     /locus_tag="ACG8KR_002018"
     CDS             complement(10674..11018)
                     /locus_tag="ACG8KR_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HFQ0201375.1"
                     /translation="MSEANQERSGQEIELIKERILAALEMVIDPELGIDIVNLGLIYD
                     IEFNPENGETVIKMTLTTMGCPLADILTESIHDALKEVPEVTKAEVKLVWYPAWTTDK
                     MSRYARIALGIR"
     gene            complement(11172..12092)
                     /locus_tag="ACG8KR_002019"
     CDS             complement(11172..12092)
                     /locus_tag="ACG8KR_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFQ0201376.1"
                     /translation="MTAYHKNERLKKIVVILITGLTAAVALNYFLIPANVFSAGMNGI
                     AQIISSLLYEWFHIKIDTGTFIFLLNIPVFVLGFLKVGKRATILSFVNVVCVSVMTML
                     LPVGQITDNILMNALVGGVLLGLGVGLSLKMGFTTGGMDIISLVLSQTTGKTVGNYML
                     ILNGIIVVIAGFFFNWESALYTIISIYAMTQVIDAIHTSHQKVTAMIVTVKPEEVTEE
                     LAKRMVRGMTLLPSVGGYSKKQGNMIMMVITRYEMYDLDQIVHEIDDDAFINILPTHS
                     VFGRFANENEQKMYRTTGVLPEIKNRSQKK"
BASE COUNT         3851 a         2392 c         1949 g         3990 t
ORIGIN      
        1 aaaaacagga actgctgcaa ataagcaatt cctgttttta tgttcattga ttaatgaata
       61 gagcccatta gttttttgat tggttctttc acagtaaata gcaagatacc accgacgatt
      121 gctactgcac cgataatacc gaagtaagca gattctgttc ccggtgtgta gaagcgtgca
      181 atttgtgcat tgattgcttg tgaagaagca tcagctaaga accaaatagc gattgtttgt
      241 gattcaaatg ctcgtggtgc taattttgtt gtcacagaca aaccaactgg tgaaagacac
      301 aattcagcaa taaccacgat gaagaaactt cccaacaacc acaatggact tacttttcca
      361 cttgtaccat aaagcatacc cggcaacatc aatagcaagt acgataatcc tgcaaataaa
      421 agtccaagag agaatttcac tactgtagat ggttgacgtt caccaagttt cgtccacatc
      481 gttacgaata ctggtgtcag aataaagatg aaaattggat tcaatgattg ataccagctt
      541 gcttggatcg ttacaccaaa tagcgtagat tgtgtacgtt cactagcaaa taaagctaag
      601 atagatgaac cttgttcttg taatgaccag aaaatgattg ctgcaaggaa taatgggata
      661 taagcccata ctcttggttt ttcttcagca gttacatctt ttgaagtcag cattttgata
      721 aagtaataca acggtaagat aatccccaat acactgattg tattgacaaa aaagtcaatt
      781 gtcagatgac cagttagttg tgccccgccg aatactgcag cgataacaac aagtgcgatg
      841 atcaagttac ggatgaatcc tttacgttct tcagttgtca aaggattcgg cggtgttgta
      901 cccactccgt ctaatgtctt gcgtccttgg atgatgtatt gtaataatcc aaagaacatc
      961 ccaatagcag ctaaagagaa ccctaaatga tagttatatt cttgaccgat tgtaccaacg
     1021 attagtggtg ctaataatgc ccctaagttg acacccatgt aaaaaatcga aaatcctgca
     1081 tctcttcgca agtcagtctt tgaataaaga tgtccgacca tgccagaaac atttggtttc
     1141 aaaaaggcgg ttccgataat aatcaatgcc atcgaaacaa acaatgtact tacaccaaat
     1201 ggtaaagcca agacgatgtg gccaagcata atgaaaactc caccgaagaa tacagtgcgt
     1261 tttgctccta acactcggtc agatacccat ccgccgacga tactagacat gtagatcaaa
     1321 gatccgtaga tcgacatgat agctaatgct gttgctcgtg gtaatccaag tcccccgttg
     1381 gcaactgtat cgtacatata atagataagt aatgcacgca taccgtaata actaaatcgt
     1441 tcccacatct cagtgaagaa caaagttgac aatcccttcg gctgcccgaa gaagcccttg
     1501 tcttgctgtt tttccataat ctatacccca attctttaaa ttttcagaaa atattataca
     1561 tctttaccga aaaatttaac taaaaaactt atttaagaaa tataaactaa ttttctataa
     1621 aagcaagtaa tttttaatgg caatatcata aaaaaaagaa gatagagatt taataattat
     1681 atatcagctg tttttttcaa ataagcaaaa attcctaaaa cacaacaatg aaagcgataa
     1741 ctaagaaaat ccttacataa cgatttttct tattaaaatt gttttttgaa cttcgcttat
     1801 tttttgtgag catatctgtt tacttacttt ttgtaaggtg ctatacttca tttcgtaatc
     1861 aattaaaaaa atgcaaaacg caaatcaaag gagaaatttt attatgacaa cttttactga
     1921 cactttgaaa aaccgccgtt caatctatca tctaggacgt aatgtttcac tttctaatga
     1981 agaattaaca actttgatca aagaagcaat taaagaaagc ccaactgctt tcaatgctca
     2041 atcaacacgc gcagttatcc ttttcggaga tgcacatgaa aaactttggg aaatcactga
     2101 agaagcactt cgcccattga ctccagcaga agctttccca aatacacaaa acaaattagc
     2161 tggtttcaaa aatggttatg gtacagtatt attcttcaaa gatactgacg tggtaaaagg
     2221 tctacaagaa caatttgaat tatatgcaga caacttccca gattggtcag aacaatcaaa
     2281 cggaatcgca acagctaata cttgggtagc attagttgat aaaggattgg gcgcaaactt
     2341 gcaacattac aacccagtta ttgacgaagc agttgcaaaa gaatggaata tcccttcaaa
     2401 ctggaaacta cgttcgcaat tagtattcgg ttcaccagaa acaccggctg gggaaaaaga
     2461 atacatgaac gatgccgatc gcttccgcgt atttggataa ttttatttga aataaaaaac
     2521 agaggttgtg acaaacatct tgtcgcaacc tctgttttta ttctggatca atcataatat
     2581 caatctctgc ctcatcgttt aagcgttggg acaaaaatgg aactaattct tctaatgatg
     2641 cagaaaattt cagcatcggt cctcgccatc cgtttcggaa aaaattcatt tttacgattt
     2701 tagaaccttt tggctgatat aatacaattt tattgatctc agaaaaagga acaccttttt
     2761 tgtcaaagct attcaaaaca agtccttctt ctgtgattcc tctactgtcc aacaaaaaac
     2821 taataaaaat cattgctgac aagatccctt ttgccaattc atctgctgta ggatgactga
     2881 tgagtgtata ccaaatgact agtgacacta agaagggtat caccaagtag acccatcgat
     2941 ttcgcacagt ccgataaata atttcttttc gaaccagcca aagaaataaa aacatcacaa
     3001 caacgaataa aaacaataga accgtaaaga atgttaccat gacaactcgc ctcgcttttt
     3061 cttctccaga aaataggaaa agaatactga tttaccttct caaagaaatt ggacttgcag
     3121 cacgccgcac gtccaattgt tttctttatt caaatgtatc atattttgca acaaccatgc
     3181 ttgctttttc atctaataat tcttggtcct ctgcagcaca ttgggcaact ttgaccacgg
     3241 cgcagtttgt catttttgat acaacttcac caatgaccgt ttttgtaaaa ccaattggtt
     3301 tgcatgcata ttgttctcct aaattaatca tttcttccgt catcaaaatc ctcctaaact
     3361 gctggaactt ttctccacat taccactgct atttctttgg agatttaact cattgatttt
     3421 tatttacagt taaaaatcaa ccttacaggc attatagcgt actgttttcg ttttcacaat
     3481 taaaacagct gttgtattta gaagaatttt tttcaaaaaa ttaaaacata ttccttaaaa
     3541 actaattatt tcctacatat taaaatataa tacttccatt tccaataaaa atacatttat
     3601 aataggaaag ataaacagct gaaaagggag atttcaaatg aagaaaaaaa cgttcaaaaa
     3661 actattgttt ctagggggca cagctgctct tttattaagc ggtacaagtt attacctaca
     3721 tatccattct ccagaacata caatgaacac tgcacaaaaa agttcaacac aaaaaacgaa
     3781 aaagacaaaa gagaaagcag aagttttact agatgttcca ttagagagcc agtttgattc
     3841 tccttcttta gaaaatggct gtgaagtcac ttcgctttct atgcttttat ctttttatgg
     3901 ttatgaaaca actaaaaacc agttagctga gcaattggat tacgtccctg ttttcaatgc
     3961 agatgggact catggcgatc ctaatgaagg atttgttggt gatatcagtg gcggagactg
     4021 ggctatggtc gtctatgttc ccccagttgc ctcccttgct cagcaaatcg tccaaacgga
     4081 ttatcacact tttccaaaaa ccgatgctca actcgatgat atcaaaaaag cactatctaa
     4141 aggaaatcct gtctggactt ctgtgactat caattttgaa gtcccagatg aaactgattt
     4201 tatgacttgg accacaaata atggagaagt aagagtcact cctcttgttc atgcttgtgt
     4261 agtcactgga tatgacaaag aaaatattta tgtcaacgat ccctatggtg taaaaaatcg
     4321 agcagtaccg atcaacgatt tttcggcgat tttcactgca atgggtggac agatgctaac
     4381 tttggagaaa agctagtaat tcaaacaaaa atcccttata ctcaagatac caaacctttg
     4441 aagggagcgt agataatgga aaaattcaca cataaaaaaa tggatccaaa tgaaattccg
     4501 attatttttg tccgtgactg caagggaaat gttcaaggta aagtctctat taatgagtgg
     4561 aacgagcgtc gcagacctgc taccctcaat gaactggaaa tcaaattgta tcgtcaatct
     4621 cttgtttatt acgcagatca agagtacgag aaagccaccg atttattgaa atttctaatt
     4681 gctcgaacag agtatactcg ctttgaatat attgaacgtt tagcaaacat ctatcatatt
     4741 atgaatgaac ctgtcaaaga atatcagtta ttggacaccg tcttgtcagt agcagaactg
     4801 attgcccttc ctgctggatt agaaaagaaa ctagtacgcc gccttttacg agtaaagcaa
     4861 caactatccg atcaagaaaa ataacttgcg tttttttagg aaagtgctat gattattttg
     4921 ttgaataaat gggatgtagc caaactggta aggcacttga ctctgaatca agcaattgta
     4981 ggttcgaacc ctgccatccc aatttgatgt gcttgctgaa aaaatgaacc ccctgtaaaa
     5041 gaacaatctt tttgcagggg gattattgta ttatttttac tactagaatt tagtttacat
     5101 aattaaatta tgaataataa aatcgggcgt attcttaata caaaaactga tttacccaat
     5161 aattgctaat gctttctccg gaactttttc tctaggaact tcctcatacg tttctatgtg
     5221 tgctccttta ttgctaattt tcaaataatg attttctttt aacacagaaa gaccattaaa
     5281 ttcaaccgtt cgttctttcc cattggcatc agcagctttc tgcacatacc ttgctgtacc
     5341 atattcattt acttcctctg gtttttgtgt ctttacatag acctctcctt tttcaactaa
     5401 tggatttagc tgatcaagta taccagggat ttctccataa cttccttcag tataatattt
     5461 cagtccaaat agcaaaatac cactaatcgc cgccagtcca atcaaatatt taatcacttt
     5521 catctttcta cctccttgtt atatattcag tatatgggtc agttaaagaa attccatccg
     5581 tcccaagttg gagatttttc tcctacttta gaagcgagaa tcgagtgttt ttttagtaaa
     5641 aatgatcgtt atttgatatt attgacgtgt ggataaatca gtttttctcg ttttgtccat
     5701 gtaaaaaatc atgttgatag caaaggatgt gttcaaaatg aatcaagaag aattatttca
     5761 aaaagtcaag gaaatgatta agaacggcaa tttcgatggc gcaaaacgat ttatcgaaga
     5821 acataaagaa caattaggac cttataaaga gaaagcccaa aatcttttaa aagatgtaaa
     5881 tatcgacagt gtcaaaaaca aatttaaaaa tctattcaaa taagtaaaac tttcactttt
     5941 gtaaaatcaa aggaggacaa tgctgctgca tcgtcctcct ttggttcttt tctgaatact
     6001 tcatactaaa ataaacgtgt cggatcgtaa tgataagtca cattgccgtt cagtgaggct
     6061 tttcccacca catatttacg attctccaac ttcagccacg cttctcgaaa agccattaat
     6121 tcttctcttt ttacatcaat cttttcgatt tttccatcga tcaaatcttg aataagttgt
     6181 tcatatggat tcattttttt cctccatctg ctgtcaatca ataagttgta aaaataggtt
     6241 catatgcttt tttggtatct agatcttctc tgactttgac tgtcgtataa ataatttttt
     6301 ctactgggat actataataa cttccttcac cattattgaa tcggtgaacg tctttctttg
     6361 ctaaaatacc atttatttta ttcgtcagta cacgttgttc gacactgtta cttacaacat
     6421 agtaaatcac ccgaatatta ttgatcaaat gcaaatcgat ttctaacact acatgatcca
     6481 tgaaaatccc actccttctt tcactttgat tatagcaagt ttctcctcta atttcaaaat
     6541 cgatacagat tttaccggat aaatccacaa aactgcttgc gccatttcgc acaaagtgtt
     6601 attttgtatc atggacttta tttatcggag gcaatagtat gatcgcgatc ggtcaatttg
     6661 ttttttatat tccttttttt attatgataa gcattctttt ctattacata aaatggacaa
     6721 aaaagaagtt ttcagtttta ctggcctctt tacctgctgt atattttact tatcaaattt
     6781 tttcttttag gcattgggaa acaacgtctg tattaataac acacatcatc gaattgacgc
     6841 tttcagtaat ttttcttatt atttggatct attttttata taagaatcag aattgaatcc
     6901 ctcgcatgtt taaaaagaaa acgattaaag atgcagcaaa tttctgccga tctttaatcg
     6961 ttttttctat tctgttatac caaaaaaatg tttgaatcgg tgaaggatcg ttccttgttt
     7021 taattctata aattcttgtg cgatcgggta cttttcttcc aaattttctt tgttcttttc
     7081 gatcaggctt ttcgcttctt catatttttt atctgcacat agtgctttta cttcctgtaa
     7141 tacttttcct gcttccattt gcctcaccct cttgccattt tattgcttta agttttattt
     7201 aagttatacg cattattcta gtttcatccc aaataacctt ttttcatttt tactcctcga
     7261 actaatcagt tttctaaagg ctgattggtt cgtctttttt atttttcctc catttcattc
     7321 tgctcttctg ttaatttatc taatgctttg attgatgctg cggctcttgc ataatcatag
     7381 ctgtataatt ccataaattt atcaggcaga acaaaccacg ctggatgata accgttagct
     7441 gtgacaggga atcctttatc ttgttggtta tctccatccc atctcagagc aacacctttt
     7501 tttcctttcc actttactaa agcgatcgag tatcccttct ttgtttgtgt gccgtcttca
     7561 ataattgcta aaatttctat tttttctttt ggcgcacgga cttcttgagg acgaaccatt
     7621 ttctattcct cccttatctc attacttcac gaccaacaca ctgcaagggg catgtgatac
     7681 gacataagaa gcggtcgatc caactaatgc ttgttgtata gcaccttttc ctgtcgctcc
     7741 cataacgatc aaatcaatat tttcttgtcc agggatgaca ttggcaatca aatttcttgg
     7801 atctccagtt tcaactattg cgtggatctc agcaatgccg tactctttgg catcattgat
     7861 ttttttcagc atggctactt ctgttctttg tttttcatct gcatagattt ttgaaaaggc
     7921 ataggcacta tgggtcagct caactttgtt gatgattgac acgataaata agtgggcttc
     7981 attgtccttt gctaagtcaa gggcttcagc gaacgctttg atcgattgtc ttgatccgtc
     8041 aactgctacc atgatgttct ggtatctttt actcattgga accgcctcct ttttctttta
     8101 ttgtaacatc tcaagcaaaa atacaaaaaa tatacgcaat atgtaaataa aatgatgaac
     8161 aaaagcaatt tctttcaagt tatttataca tttcttttct catcagaaca cctgttctaa
     8221 tcccattatt tctgcattat gtgaaattcc tcatcatttt tcacatcgag cgaacatcat
     8281 tctgttacaa aaaacgtgga tttgtcataa attgtcatgc ttcttttacc tatgaaatat
     8341 tttcacgtgt taaagtattc ctcgttgaac acaacaaata aaacttttta acgaataaag
     8401 gagatttttc atgacatcac ttaaaacttt actttttgga acaactttgg ctgccggcgc
     8461 tgcattcttc atgggaacga ctgctcatgc ggacgaagct tataccgttc aatcaggtga
     8521 tactctatca acgatttctc aaaaatacgt tggtgataac tccttgatca atgccattgc
     8581 agaatcaaat tcaatttctg atattaacct gatctactca ggacaacaat taacaatccc
     8641 aacagaaggc tctgcacaag cagctgctga acctcaagca gccgttcaag aagcaccagt
     8701 ccaagctgaa cctgttcaag ctgaacaacc tgttgtacaa gaaacggttc aaacggaaac
     8761 acaagcagcg cctgttgctg aaacacaacc tgctcctgct gtaacagaaa ctgctgctac
     8821 tccagcaagt acaagctctg caaaagaatg gatcgcacaa aaagaatcaa gcggttctta
     8881 cacagcaacg aacggacgtt acatcggccg ttaccaatta gattcttctt acttaaatgg
     8941 tgactactct gctgcaaacc aagaaagagt agcagaacaa tatgttactt cacgttatgg
     9001 ttcttgggaa gcggcgaaag cattctggga agcaaacggt tggtactaag attcattatt
     9061 tgaacgttta aacacattca aaatatagta agaggtcatg cgatttattc tttcgcatga
     9121 cctctttttg agtttaagtt tagtctttat tccttaacta tcgtttgcca gtatttcttg
     9181 tatcagaatc tataaatcta ctatttgaat caatctatta ggttttaaac tgtcccacct
     9241 tcacacaaat tttttttgat ttatttgaat ctggttccgt tgtttaaaat ttttcctata
     9301 ttcttttaga taatatgtta ttttctttct catcgctcca ggtgtacctt ctctttataa
     9361 aaaaacaaat tatgcggcta agaagactcg aacttccacg aggtcaccct cactagcgcc
     9421 tgaagctagc gcgtctgcca attccgccat agccgcatca catcttttag tttaggatta
     9481 catgtgtttt ttgtcaagaa catatttttg ttctttttca tttctgtacg ccctccctta
     9541 tgccatctac acaaataatt catccgagca actccatcat ctattgaggg ttggtgaaaa
     9601 aatctgtttt atccatatga tcgatttcaa tgttaaacga acgactctgg gattagtaac
     9661 tattttattt ttttcttcat ttcttttttc ctctttttct cgattcagct atcttctttc
     9721 atttggatat tgattatttc ttctgttgat tactatcctt ctggaattct agacgctgaa
     9781 gtaattcacg ttttctttct tctagagatc ataagtaacg aatgaacgaa cttcaaataa
     9841 tttttttatt gtaattcata tttgtattgt tcgatgattc ttaatacgat atgacacaat
     9901 aaataaaaat aggcatggct atttacaaca atagtgctat agtagaaaac aaatgaataa
     9961 ggttacttaa agaggtgaat acgatgaaac tgatcaaact atgggaagca gatttaaaga
    10021 aagcttatca gctacaaact agctttggac caaatgagag tggatttatc aacaatgctt
    10081 atggctatac atttgctgaa tttttagatt atgtaaaaaa atgcgaaaaa acgtctttag
    10141 gaatcgatct tcctgagagt tacgttcccg caaccgtctt tattttagaa agtgatgaac
    10201 atgagtatgt aggaattttt aatttgcgcc ataaactgaa cgaaacatta agaaacggcg
    10261 ctggccatat tggttacggc atctccccta attatcgtaa aaaaggatat gccacacaag
    10321 gattagcatt agtactacaa gaggcaaaaa aaatcggcat tgatgaggca cttttgtctg
    10381 taaataaaaa aaatcttggc agtcttaaag cacaacaaaa aaatggggct gcgatttatc
    10441 gtgaagatga gaaagaatac tatacgaaaa tcaaactgat gtaaaaaaga aggattccaa
    10501 aaggaaaggg atttgaatgt cctttatcaa tagaacacat ttatttaatt aacttataat
    10561 atagcagaca aaattttata aacaaagaaa ccttttatct cttttcttct agaaaggata
    10621 aaaggctttt ttgtttttcc tatttgcttc tcatagctag tcagccaaga tttctatcgt
    10681 atccccaacg caatccgtgc ataacggctc atcttatctg tcgtccatgc tggataccag
    10741 actagtttga cttctgcttt cgttacttct ggcacttctt ttaatgcgtc atggatagat
    10801 tctgttaaaa tatctgctaa tggacagccc atagttgtta gagtcatctt aatgactgtt
    10861 tctccatttt caggattgaa ttctatatca taaatcaagc ctaaattgac gatgtcgatt
    10921 cctaattcag gatcaatcac catttccaag gcagcaagaa tacgttcttt gattagttct
    10981 atttcttgtc cagaccgttc ttggtttgct tcgctcatcg tacgacactc cttaacacga
    11041 tttttattta tcatactctt tttttagctg cttgtaaatg agaatttaga agtaagattc
    11101 tcgtttaaat ttataaaaat gttcttatta gatgtaatag attaaattta ttctaaaata
    11161 aacctgattc ctcatttttt ttgtgaacga ttttttattt ctggcaatac gccagttgtt
    11221 cgatacattt tttgttcgtt ttcatttgca aaccggccaa atactgaatg agttggtaaa
    11281 atattgataa atgcatcgtc gtcgatttca tgaacgatct gatcaagatc atacatttcg
    11341 tagcgggtga tgaccatcat gatcatgttc ccttgttttt ttgaatagcc accgacagac
    11401 ggcagtaatg tcattccacg taccatccgt tttgctagtt cttctgttac ctcttctggc
    11461 ttgacggtaa cgatcattgc tgttactttt tgatgactcg tgtggatggc atcaattact
    11521 tgagtcattg cataaatgga aataatcgta tacaaggcac tttcccagtt aaagaagaag
    11581 cctgcgatga ctacaatgat accatttaga atcagcatgt agtttccgac tgtttttcca
    11641 gttgtttgtg aaagtacaag agagatgata tccatcccac cagttgtaaa gcccattttt
    11701 aaagataatc ctacgccaag acctaataaa acaccgccta ctagggcatt catcaaaata
    11761 ttatcagtga tctgtccgac tggaagcagc atcgtcatca ccgatacaca tacaacattc
    11821 acaaaactaa ggatcgttgc tcgttttcct actttgagaa accctaatac aaacacaggg
    11881 atattcaaaa gaaaaataaa tgttcctgtg tctatcttga tatgaaacca ttcatataaa
    11941 agcgaggaaa tgatttgagc gatcccattc atccctgcag aaaagacatt ggctggaatc
    12001 aaaaaataat ttaaagcgac cgcagcggtc aatccggtta tcaaaataac cactattttc
    12061 tttaatcttt catttttatg atacgcagtc ataattttac tcacaagcac ctctccccta
    12121 ctttggtatt ccaaatgtaa gaataacagt ttatctatta aaataaaata cttcgaactc
    12181 aa
//
LOCUS       DAZKLD010000094        12178 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.094, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000094 DAZKLD010000000
VERSION     DAZKLD010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12178)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12178)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12178
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.094"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(140..304)
                     /locus_tag="ACG8KR_002020"
     CDS             complement(140..304)
                     /locus_tag="ACG8KR_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0201377.1"
                     /translation="MKNLIAGGFLFVGGGILMNTDNFGNVIPFLGAACALIGVGMLIY
                     FVFSDDGKYG"
     gene            599..1927
                     /locus_tag="ACG8KR_002021"
     CDS             599..1927
                     /locus_tag="ACG8KR_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HFQ0201378.1"
                     /translation="MKAITPDMTTKFNKQFQANIKQQALQRAVVKNGITASAENVSAH
                     VENTPVFSIDLATGKVANQMQSGRCWMFAALNTFRHKMLNQFHLKEFELSQNYTFFWD
                     KYEKANYFYENIIATGKEAIDSRKVAFLLATPQQDGGQWDMIVSLFQKYGVVPKSVMP
                     ESANSSNSRDLNNYLNKLLRKDAVVLRKMVAQGETLDAIEEKKEEMLESIYNFLAISL
                     GTPPKEFDFEYRDEEKNYHLDRGLTPQIFYDKYIGVKLDDYVSVINAPTKDKPFNRSY
                     TVEMLGNVVGGKEVKYLNVDMETFKKLAIAQLEEGESVWFGCDVGQSSTRTSGIMALD
                     AYSMDDLFDTDFTMTKAERLDFGESLMTHAMVLTGVDLVDGQSTKWKVENSWGEKVGK
                     NGFFVMSDAWMDEYTYQIVVRKDLLTKEQLNAFNEEPIVLSPWDPMGALA"
     gene            2129..2962
                     /locus_tag="ACG8KR_002022"
     CDS             2129..2962
                     /locus_tag="ACG8KR_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HFQ0201379.1"
                     /translation="MDIQTVLSDLKLNGKVIPVVGGDVNQTYRIKTEHRAYFLKIHPN
                     VKKGFFEAEVDGLKELSAFVRVPDTYMLGETSEGAYLLMEWIEPGKGDQRDLAAALAN
                     LHQQTAPQFGFRKDNYLGTLVQKNSFEEDWWTFFFKDRLESQISLAEETNRWNVQRQE
                     KYLRFKERVLKSVEPKKITPRLLHGDLWSGNVFFDQQGHPVFVDPAVSYGNREQDIAM
                     SQLFGGFRPEFLDAYQTIFPLEKGWKDRLPIYQLYYLLAHLNMFGESYGSQVDQLLEN
                     F"
     gene            2978..3715
                     /locus_tag="ACG8KR_002023"
     CDS             2978..3715
                     /locus_tag="ACG8KR_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP thioesterase domain-containing protein"
                     /protein_id="HFQ0201380.1"
                     /translation="MAKKYTKKHEVSYYECDINQTMTFPSMLGVVIKTSEDQSDQLGR
                     GSEFVNSFGLTWVITNYSMEINRLPRVGEVISVTTQAMEYNKFFCYRNFWIHDEDGKE
                     LVKIESVFVLMDFVNRKMSSVNEEIIAPFESEKIKKIKRQEKIEKIDSGFMLPYRVRF
                     YDIDSNQHVNNAMYFNWIIDVLGYDFLTTHVPEKVIIRFDKEVEYGNEIESHFEQIPI
                     ENGVKTKHEIRMQEQLYCEANIIWKNK"
     gene            complement(3870..4838)
                     /locus_tag="ACG8KR_002024"
     CDS             complement(3870..4838)
                     /locus_tag="ACG8KR_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HFQ0201381.1"
                     /translation="MKKILVLHTGGTIAMKENMLTGGVSPDVANPLLDAEITIPKHVE
                     LLVEDIFNLPSPHIMPSHMLVLKERILSAYASGISGVVITHGTDTLEETAFFLDTTIG
                     NKLPIVLTGAMRSSNELGSDGLYNFESAIRVASCEEALDKGVLVVMNDEIHSARYVTK
                     THTTNVATFRTPTLGPIGLVTKNRILFLQELLETKRLDISAVDGTIPIVKAYAGMQGD
                     LLEAIAHTKVDGLVIEALGAGNLPPQALAALEKLLVQKIPVVLVSRCFNGIAEPVYDY
                     EGGGKELEKMGIIFCNSINSQKARLKLLIAVNYGLSGEELISFIQN"
     gene            5070..5903
                     /locus_tag="ACG8KR_002025"
     CDS             5070..5903
                     /locus_tag="ACG8KR_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HFQ0201382.1"
                     /translation="MIRIGLTSFKEHDKLTGKKNNTLYEYAGFLPLVELDTAYYGIPR
                     KSSVENWISEVPESFRFVVKVYQGISGQGDWKPYYQTEEEMIAHFLDVMSPMVDAGKM
                     FCFLVQFPSSFKCTKENVQYLRKLRKWFEGYPVSIELRDYSWYSDEFLERTRNFMSRA
                     AFSLAIIDEPQLLNTTIPFDPFVTNPNFALFRFHGRNAQGWQANDKEWRKKRTLYRYS
                     EKELQELAHEVKVVDSKTKEVGVIFNNNSGGDAAENALALQKILNIKPDGLNPTQMDL
                     F"
     gene            5937..6776
                     /locus_tag="ACG8KR_002026"
     CDS             5937..6776
                     /locus_tag="ACG8KR_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFQ0201383.1"
                     /translation="MIKAIFFDIDGTLVTNRSKALESTKQAIEYARKNGILCGVATGR
                     SPVKIKEIIDELELDMYVVYNGQLVFTADRTIIDHPFEQKVLEHIVEFADENHRQIVF
                     GARNRLDGSTTMLLGQSIFIKRLVSFLPRKFPVRLMKKILQVFSPHRQKDRYETLEIL
                     KEPIYQCILLSPESEQKKLEHRFPECTFQRSNTYTVDIIPKGGSKLLGIQAFANAVEI
                     EMEEIMAFGDHYNDIEMLKGVGIGVAMGNAQIEVKHAADFVTQSNEEDGIYEGLKHFG
                     LIY"
     gene            6802..7362
                     /locus_tag="ACG8KR_002027"
     CDS             6802..7362
                     /locus_tag="ACG8KR_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFQ0201384.1"
                     /translation="MEAETPFQKVETFHKTFDPRRPEIPTAFTENEAHTRAAFKIEEL
                     VEFVAATSPNKEQLHEAVMLLHEALDKAEEKILGKTQWGSDPLVEQVDALTDILYFTY
                     GSFSLLGIVPDRIFEIVHQANMGKLFPDGKPHYDPVTNKVLKPENWQMEYAPEPKIAA
                     EIQRQIQEAIAKKKSADIGMCTNEKE"
     gene            7561..9282
                     /locus_tag="ACG8KR_002028"
     CDS             7561..9282
                     /locus_tag="ACG8KR_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HFQ0201385.1"
                     /translation="MGNNVIIGIVIAIIIVAIVVFLVSYLMKKKNQDRLDALEKRKEE
                     LFDLPVIEEVDDVKKMHLVGQSQNTFREWSQKWNDISTSSFAELESQIFEVENLNESF
                     RFLKGKKAVVEAEATMDQMEKQVTEIREGLKELRETEERNSLAVQQALDVYEELKEKV
                     REDEHEYGPALFELQKQIKVIEKEFTQFVTLNTSGDPVEAREVLDKAEKHTYEVEALM
                     KKIPPLYEELHTTFPEQLEEISDTYEKLMEEQYVFPEEDLAEDIAKVSRRIENSLANL
                     EKTEVETVEFENRETADLIDSLYDILEREMEAQRYVKTNQSTIAEYIKHTTKNNRQLL
                     IELDHTSQSYTLNHNEIGRVRGFQTEVEEMERQNEQMIPQIRQHEIPYSEVRTFYKTV
                     FKVLEDIEKQQVEIDDSLHELRKGEKEAQEKIDTFEFKLRSLKRFVEKQRLPGLPNDY
                     LEFFFVATDRIEELSVVLNKIRVNMEEVNRLVALCEEDLELLDKKTHDLVDAAALTEQ
                     MLQYANRYRHTHEEVREAIDKSYYLFNKEYHYQEALDEIGTALERVEPGAFKRIEDFY
                     FNHPDLV"
     gene            9447..10589
                     /locus_tag="ACG8KR_002029"
     CDS             9447..10589
                     /locus_tag="ACG8KR_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HFQ0201386.1"
                     /translation="MIYFDNSATTPIFPQSLDAYVKTSQRIIGNPSSLHDLGNQANRL
                     LQQARKQIADLIHVEPEEIYFTSGGTEGDNWVLKGTMIEKREYGNHMIISGVEHPAVS
                     ETAEQLKELGIEVSIAPVDKRGFVRVDELKELIRKETVLVSVMAVNNEVGSIQPIQEI
                     SDLLDDFPKIHFHVDAVQAIGKVPFSQWLTERVDFATFSAHKFHGPRGTGFIYWKKGR
                     RLAPLLTGGGQERNQRSGTENVPGIVAMVKALRLSLEIKEQKPEQTQRLKVALINALK
                     TYEKVTIFSEGTDYAPHILCFALKGIRGEVLVHALEEKQIFVSTTSACSSRKHMASST
                     LHAMHVPNDLATSAVRISLDENNSLAEVEQFMIIFKQLYKKFSAIN"
     gene            10605..11816
                     /gene="thiI"
                     /locus_tag="ACG8KR_002030"
     CDS             10605..11816
                     /gene="thiI"
                     /locus_tag="ACG8KR_002030"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA-uracil-4 sulfurtransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HFQ0201387.1"
                     /translation="MKYSEIMVRYGELSTKGKNRKSFIMQLAQNVRQALKDFPEIKIH
                     ADRDRMHLLLNGADGQLVIPKLAKIFGIQNFSPSIRVEKDVEVLKQAVQDIMKEIYTG
                     KETFKIIAKRSDHQFELTSNELNQTLGNAVFDIFPHIQVQMRQPDIPLRVEIRRDGAY
                     LSYETIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYFAMKRGVEIEAVHFASPPYTSEQ
                     ALQKAKDLTAKLAPYVGTIHFIEVPFTEIQEEIKKSVPQGYWMTITRRMMLRLTDEIR
                     RIRHGLVILNGESLGQVASQTLQSMVAINEVTNTPIIRPVATMDKLEIIEVAEQIDTF
                     NLSIQPFEDCCTIFAPPQPKTRPQLEKVHQYEERLAIDEMIQRALAGLKMEEIQAESS
                     NEKTEEFSELL"
BASE COUNT         4152 a         2042 c         2528 g         3456 t
ORIGIN      
        1 agtaaataaa gcttatgcaa agaaaaagaa atccaatcac ttgggtttct ttttctttta
       61 ttattatgat gaatcaagct ttctgttttt gtcacgtatg ttgtaatctt tacgaaagag
      121 tagagttggc tactcataat caaccatact tcccatcatc gctaaatacg aaataaatca
      181 gcattcctac accaatcaaa gcacaagctg caccaagaaa agggatgaca ttcccaaagt
      241 tatctgtatt cataagtatg ccaccaccca caaacaaaaa gccgccagca atcaggtttt
      301 tcattttatt catctccttt cttacgttat agtatttcct tctttcaatg agtattttga
      361 aagctatctt attgtatgat gttcttctag gcctcttctt tattatacag gccattttcg
      421 ttatctcata gaaatattcc tttttatctg aaaactattg tataccgtat gacatttttc
      481 ttcatgaagt tgtaagtata ttctcattca aatgaaaatt tcatcataga agagattgta
      541 tttctaaatt cttaagagta aaatacgaag tataacttgg atttggaagg atgattggat
      601 gaaagctatt acgcctgata tgacaacaaa atttaacaaa cagtttcaag caaatatcaa
      661 acagcaggca ttgcagcggg cagttgtgaa gaacggaatt actgcttctg ctgaaaatgt
      721 ttcagcacat gtggagaata caccggtatt ttctatagat ttggctacgg ggaaagtggc
      781 taatcaaatg caatcggggc gttgctggat gtttgctgca ctgaacacat tccgtcataa
      841 gatgctgaat caattccatt taaaagagtt tgaattatca caaaattaca cttttttctg
      901 ggataaatat gaaaaggcaa attatttcta tgaaaacatc attgcaacag gaaaagaggc
      961 aatcgatagc cggaaagtag ctttcttact cgctactcct caacaagatg gcggccaatg
     1021 ggatatgatc gtttcattat tccaaaaata tggcgttgtg cctaaatcgg tgatgccgga
     1081 aagtgcgaat agttcaaatt ctcgagattt gaataactac ttgaacaaat tactgcgtaa
     1141 agatgcagta gtgttacgga aaatggttgc tcaaggagaa acattagacg ctattgaaga
     1201 aaagaaagaa gaaatgctag aaagcattta taacttctta gctatctcat taggaacacc
     1261 tccaaaagaa ttcgattttg agtaccgcga tgaagaaaag aactatcatc ttgaccgagg
     1321 actgacacca caaatttttt atgataaata catcggtgta aaattagatg attatgttag
     1381 cgtgatcaat gcaccaacaa aagacaaacc attcaatcgt tcttatacag tagagatgct
     1441 aggaaatgtc gttggcggga aagaagtcaa atatctgaat gttgatatgg aaactttcaa
     1501 aaaattagcg attgctcagc ttgaagaagg agaatctgtt tggtttggtt gtgatgtagg
     1561 tcaatcttct actagaacta gtggcatcat ggccttagat gcttattcaa tggatgatct
     1621 atttgatacg gattttacaa tgactaaagc tgaacgttta gattttggag aaagtctgat
     1681 gacccatgcg atggtattga ccggtgttga cttagtagac gggcaatcaa caaaatggaa
     1741 agtcgaaaat agctggggcg aaaaagttgg aaagaatggc ttctttgtta tgagtgatgc
     1801 ctggatggac gaatatacgt accaaatcgt tgtaagaaaa gatttattga cgaaagagca
     1861 attaaatgcg ttcaatgaag aaccaattgt tctatctccg tgggatccaa tgggtgcttt
     1921 agcatagacg agtatttcaa tgaagagtat gtagtaaaga taaaaagagc tggcttaagc
     1981 cagctctttt tatctttaaa gtccattccc tgttttttat ggtccctgtg ctataattct
     2041 aaataaagga taaggattgg aaataatcct tttatcatta aaaatatgat gacgaagtag
     2101 aataaaattg ggagtagggg ggaaagtcat ggatatccaa actgttttat ctgatctgaa
     2161 attaaatggt aaagtgattc cagttgttgg tggcgatgtt aatcagacct atcgtataaa
     2221 aaccgaacat cgtgcttatt ttttaaaaat ccatccgaat gtaaaaaaag gattttttga
     2281 agcagaagta gacggattaa aagaactgtc agcattcgta cgtgtaccgg atacgtatat
     2341 gctaggtgaa acaagtgaag gcgcttattt gttaatggaa tggatcgagc cgggaaaagg
     2401 ggatcaacga gatcttgctg cagctttagc taatctccat cagcaaacag cgccacaatt
     2461 tggttttcgt aaagataact atttgggtac acttgttcag aaaaattctt ttgaagaaga
     2521 ttggtggact tttttcttta aagatcggct tgaatcccag atttctttgg cagaagaaac
     2581 gaatcgatgg aatgtacagc gtcaagaaaa gtatctgcga tttaaagaac gtgtattgaa
     2641 atcggttgaa ccaaaaaaga tcactccacg cctcttgcac ggagacctgt ggagtggtaa
     2701 tgtgttcttt gaccagcaag ggcatccagt ttttgtcgac ccagctgttt cttatgggaa
     2761 tcgggaacaa gatattgcaa tgagccagtt attcggtggt ttccgaccgg aatttttaga
     2821 tgcttatcaa actatatttc cattagaaaa gggatggaaa gaccgactgc cgatttacca
     2881 gttatattat cttttggcac atttgaatat gtttggagaa agttacggtt cccaagtaga
     2941 tcaattacta gaaaattttt aagaggaggg cgtgtttttg gcaaaaaaat ataccaaaaa
     3001 gcatgaagtt tcatattatg agtgtgacat caaccaaacg atgacatttc cttcgatgtt
     3061 aggagtagtt atcaaaacgt ctgaagatca aagcgaccag ttgggaagag gcagtgaatt
     3121 cgtcaatagc tttggactta cttgggtcat cacgaattat tccatggaga ttaatcgtct
     3181 ccctagagtc ggtgaagtaa tctcagtaac gactcaagcc atggagtaca ataagttttt
     3241 ctgttatcgg aacttctgga tccatgatga agatgggaaa gaattagtca aaatagaatc
     3301 tgttttcgta ttgatggatt ttgtgaatcg aaaaatgagc agtgttaatg aagagatcat
     3361 tgctcctttt gagagtgaga agatcaagaa aatcaagcgt caggaaaaaa ttgaaaagat
     3421 tgattcgggc ttcatgttgc catatcgtgt tcgtttttat gatatcgata gtaatcagca
     3481 tgttaataat gcgatgtatt ttaattggat cattgacgta ttaggatatg actttttgac
     3541 tacgcatgtt ccagaaaaag taattattcg atttgataaa gaagtagagt acgggaatga
     3601 gatcgaaagc cattttgaac aaatccctat cgaaaatggt gtcaaaacaa aacatgaaat
     3661 ccgaatgcaa gaacagctgt attgtgaagc aaatattata tggaaaaata aataaaaaag
     3721 aggttgtgac agaagggggc aacttcaaga aataagaaga aatttacgaa aattgtttct
     3781 caaatttttg taaattttcg acttagtttt cgaagaagct atttttgtca cagcctctct
     3841 ttttaagttt agctattaga taatgatgtt tagttttgga taaacgaaat caattcttca
     3901 cccgacaaac catagtttac agcaatcaat agttttaaac gtgctttttg actattaata
     3961 ctgttacaga aaataatccc cattttttct agttcttttc ccccgccttc ataatcataa
     4021 acaggctctg cgattccatt gaaacaacga gaaacaagta ctacagggat tttttggaca
     4081 agaagctttt ccaaagcagc taaggcttgt ggagggagat tccccgcacc taaagcttcg
     4141 attaccaaac cgtctacttt cgtatgggcg atggcttcca ataagtcccc ttgcatccca
     4201 gcatatgctt taacgatcgg gatagtccca tctacagcgg aaatgtccag tcgctttgtt
     4261 tctaaaagtt cctgcaagaa taaaattcga ttcttagtta ctaagccgat gggacccaat
     4321 gtaggtgttc ggaaagtagc aacatttgtg gtatgggtct ttgtcacgta gcgtgcagaa
     4381 tggatctcat cgttcatgac caccaaaacc cccttatcta gcgcttcttc acaacttgcg
     4441 acacgaatcg cactttcaaa attataaagg ccgtcacttc caagttcatt acttgaacgc
     4501 attgccccag tcaaaacaat tggtaatttg ttgccaatcg ttgtatcaag aaaaaaagca
     4561 gtttcttcta atgtatctgt accgtgtgtg atcaccactc cggatatacc tgatgcataa
     4621 gcggacaaga tacgttcttt taataccaac atgtgagaag gcatgatgtg tggtgaggga
     4681 agattgaaga tatcttcaac taataattct acatgcttgg ggattgtgat ttctgcatct
     4741 aataatgggt ttgccacatc aggactaaca ccgcccgtga gcatgttttc tttcatggcg
     4801 atcgttccgc cggtgtgtag tacaagtatt tttttcattt tacagacttc cttacgaata
     4861 attttgcata gccgttaaat cttattgtat actatataaa agagttttgc tatcagtgaa
     4921 gaataacgaa tagcctaact tatcataaaa aagctgacct tattaaaatg gaaaagactc
     4981 aaccttgacc tcctaataca atggagtatc tggttgagtt tcttttacaa gaaagcagcc
     5041 aaggaaatca atcacgaagg tggccggaaa tgatcagaat cggattaaca tcgtttaaag
     5101 aacatgacaa attaactggt aaaaagaata acactcttta tgaatatgca gggtttctac
     5161 ccttagtgga attagatact gcctattatg gaattccccg aaagtcctca gttgagaatt
     5221 ggataagtga agtacctgaa tcatttcgct ttgtagtcaa agtctatcaa ggaatcagcg
     5281 gtcaaggaga ctggaagcct tattatcaga ccgaagaaga gatgattgcg cattttttag
     5341 atgttatgtc gccaatggta gatgctggaa agatgttttg ctttttagtc caattcccct
     5401 catcattcaa atgtacgaaa gaaaatgtcc agtacttgcg caaacttaga aagtggtttg
     5461 agggttatcc tgtttctata gaattgcggg attattcttg gtacagcgat gaatttcttg
     5521 aacgtacaag aaactttatg tctagagccg ctttttcttt agccatcatc gatgagccac
     5581 agttgctgaa tacaacgatt ccttttgatc catttgtcac caatccgaac tttgctcttt
     5641 ttcgattcca tggtaggaat gcgcaaggat ggcaagcaaa tgataaagag tggcgtaaaa
     5701 aaagaacgtt atatcgttat tcagaaaaag aactgcaaga actagcacat gaagtcaaag
     5761 tagttgattc taagacaaaa gaggtaggtg tgatttttaa taataattct ggtggtgatg
     5821 cggctgaaaa tgcattagca ctgcaaaaaa tattaaatat caagccagat ggactcaatc
     5881 caacccaaat ggatctgttt taagggctta ttttattcaa gaaaggggcg aagtctatga
     5941 tcaaagctat tttttttgat attgacggaa cattggtcac caatcgttca aaagcgttgg
     6001 aatcaacaaa acaagcaatt gaatatgctc ggaaaaacgg cattttatgc ggtgtagcaa
     6061 cggggcgcag cccagtaaag atcaaagaaa tcattgatga attggaatta gatatgtatg
     6121 tcgtgtataa tggtcaactg gtttttactg cagatcgcac gattatcgac cacccttttg
     6181 aacaaaaagt attggaacat attgttgaat ttgcagatga gaaccatcga caaatcgttt
     6241 ttggtgcgag aaatcggtta gatggatcaa cgacgatgct attaggacaa tcgattttta
     6301 tcaagcgact agtcagcttt ttaccgcgta aattcccggt acgtctgatg aaaaaaatcc
     6361 tccaggtttt tagccctcac cgacaaaagg atcgttatga aacgttagag atattaaaag
     6421 aaccaattta tcaatgtata ttgctcagtc cggagtcaga acagaaaaag ttagaacatc
     6481 gctttcccga gtgtacgttt cagcgttcaa atacgtatac tgtagatatc atcccaaaag
     6541 gtggttcaaa attactaggc atccaagctt tcgcaaatgc tgtcgaaatt gaaatggaag
     6601 aaattatggc attcggcgat cattacaatg atatcgagat gctgaaaggt gtaggaattg
     6661 gagtggccat ggggaatgct cagatcgaag taaaacatgc tgcagacttt gtgactcaat
     6721 caaatgaaga agatgggata tacgaaggac tgaaacactt cggtctgatt tattagtcaa
     6781 tgaaagaagg aaaaaatcag tatggaagca gagacacctt ttcaaaaagt agaaacattc
     6841 cacaaaactt ttgatccaag aagaccagag atacccacgg cttttacaga aaatgaagct
     6901 catacacgtg ctgcgttcaa aatagaagaa cttgttgaat ttgttgcagc tacttcacct
     6961 aataaagaac aactgcatga agctgttatg ctgctgcacg aagcattaga taaagcagaa
     7021 gaaaaaatac ttggtaaaac acagtgggga agtgatccgc tggttgaaca agtagatgca
     7081 ctgacagata tcctttactt tacttatggt tctttttcat tactaggaat agtgcctgat
     7141 cgaatattcg aaatcgtcca tcaggcaaat atggggaaat tatttccaga tggaaaacct
     7201 cattatgacc ctgtaacaaa taaagtgttg aaacctgaaa actggcagat ggaatatgct
     7261 ccagaaccta aaatcgcggc agaaatccaa cgtcagattc aagaagcgat tgctaagaag
     7321 aaatccgctg atataggcat gtgcacaaat gaaaaagagt gatcagagtt tcactatttt
     7381 tgttatcttt atacgatgga aaaacaaaag attttcgttt aagttagtga atgcatgaaa
     7441 atagtatgct ataatctaga atagatgtct tttgcacgag agacgtatta tttggattta
     7501 tgccaagcag aacgccatct gttgcataaa agatgcatac tgaaaacgga ggaaccataa
     7561 atgggaaata atgttatcat cggaatcgtc attgcaatca ttatagtcgc cattgtcgtc
     7621 tttttggtta gttacttgat gaagaaaaaa aaccaagatc gattggatgc cttagaaaaa
     7681 cgcaaggaag aattatttga cttgccagtg attgaagaag tcgatgatgt taaaaaaatg
     7741 catctggttg ggcaaagcca aaataccttt cgtgaatgga gtcaaaaatg gaatgacata
     7801 tctacttcct cgttcgctga actagaaagt caaattttcg aagtggaaaa cctcaatgaa
     7861 agttttcggt tcttaaaagg aaaaaaagca gttgttgaag cagaagctac aatggaccaa
     7921 atggagaaac aagtgacgga aatccgtgaa ggtctaaagg aattgcggga aacggaagaa
     7981 cgcaactcac tagctgttca gcaggcactt gacgtctatg aagaactgaa agaaaaagtc
     8041 agagaagacg agcatgaata cggaccagct ctttttgaat tgcaaaaaca gatcaaagtg
     8101 atcgaaaaag aatttacgca gtttgtgact ttaaacactt ctggagaccc tgttgaagcc
     8161 cgcgaggttt tggataaagc agaaaaacat acgtatgaag tggaagctct gatgaaaaaa
     8221 attccaccgc tgtatgagga gctccatacc actttccctg aacaacttga agaaatcagt
     8281 gatacttacg agaaattgat ggaagaacaa tatgtattcc cagaagaaga tcttgctgaa
     8341 gatatcgcaa aagtaagtcg aagaattgaa aattcattag ccaatcttga aaaaacagaa
     8401 gttgaaacgg tcgaatttga aaaccgtgaa acagctgatt tgattgattc gttatatgat
     8461 atcttagaac gggaaatgga agctcagaga tatgtaaaaa ctaaccaatc aacgattgca
     8521 gagtatatca aacatacaac aaagaataac cgtcaattat tgattgagct ggatcacaca
     8581 tctcaaagct atacattgaa ccacaatgaa attggccgtg tacgaggttt ccaaaccgaa
     8641 gtggaagaaa tggaacgtca aaatgaacag atgattccac agatccgtca acacgaaatt
     8701 ccttattcag aagtgcgcac gttctataaa acagtcttca aagtcttaga agatatcgaa
     8761 aagcaacaag tagagatcga cgactcatta catgaattga gaaaaggcga aaaagaagcg
     8821 caagaaaaga tcgatacatt cgaattcaaa ttgcgcagtt tgaaacgttt tgtagaaaaa
     8881 caacgtttgc ctggcttgcc gaatgattac ctagaattct tttttgttgc tactgaccgt
     8941 attgaagaac taagtgttgt cttgaataaa attcgagtaa acatggaaga ggtcaatcgt
     9001 ttagttgctc tatgtgaaga agatctagaa ttattagata agaagacaca tgatttagta
     9061 gatgcagcag cattaacgga gcaaatgctc caatatgcaa atcgttatcg tcatacacac
     9121 gaagaagtga gagaagcaat tgataaaagc tactatttat tcaataaaga atatcactat
     9181 caagaagcat tagacgaaat cggtacagca ttagagcgag tagaacctgg tgctttcaaa
     9241 cgcatcgaag atttttattt taatcatcca gacttagtat aaagcaaaaa agttagtaaa
     9301 ccagcaagag caagacggat tattgtcttg ttcttgtctt tttttgtata tttgaaaatt
     9361 gaaacaagtg aaaatttcac ctgctcttga aagcaccttg gacaaccgtt ataatagaaa
     9421 agaatttcaa aaaagagggg tttcttatga tatattttga taacagtgca acaaccccaa
     9481 tctttccgca aagcttagat gcatatgtaa aaaccagtca gcggattatt gggaatccat
     9541 caagcttgca tgatttaggc aatcaagcta accggttgtt gcagcaagca agaaagcaga
     9601 tcgctgattt gatccatgta gaaccagaag aaatctattt tacttcaggc ggaacagaag
     9661 gagataactg ggtattaaaa ggaacgatga tcgaaaaaag agaatatggg aatcatatga
     9721 tcatttcagg agtcgaacat ccggccgtca gcgagacagc agaacaatta aaagaattag
     9781 gaatcgaagt aagtatcgcc ccagtggata aacgcggatt tgtacgtgtc gacgaattga
     9841 aagaattaat aagaaaagag acagtattag tctctgttat ggctgtgaat aatgaagtag
     9901 gatcgattca gcccatccag gaaatcagtg atcttttgga tgattttcct aaaattcatt
     9961 tccatgtaga tgctgttcag gcgattggaa aagtaccatt ctcgcaatgg ttgacagaaa
    10021 gagttgattt tgcaacattt tcagctcata aattccatgg cccacgggga actggtttca
    10081 tttattggaa aaagggtcgt cgtttagcac cattactgac aggtggaggg caagaaagaa
    10141 atcagcgaag tgggacagaa aatgttccag gtattgtggc tatggttaaa gctttacgtt
    10201 tatcattaga gataaaggaa caaaaacctg agcagacaca acgcttaaaa gtagctctaa
    10261 tcaatgcgtt gaagacgtat gagaaagtga cgattttttc agaaggaact gactatgcac
    10321 cgcatatttt atgttttgct ctaaaaggaa ttcgcggcga agttctcgtc catgccttag
    10381 aagaaaaaca aatatttgtt tctactacta gtgcctgctc ctcacgtaaa catatggcaa
    10441 gcagtacatt gcacgcaatg catgtaccaa atgatctggc tacatctgct gttcgaatca
    10501 gcttagatga aaacaattcg cttgcagaag tcgagcaatt tatgattatt ttcaagcagc
    10561 tttataagaa attttctgca atcaattaaa agaggagtgt tcttgtgaaa tacagtgaaa
    10621 ttatggtccg ttatggggaa ctttcgacaa aaggaaagaa tcgaaaaagc tttatcatgc
    10681 aactagcaca aaatgtcaga caagctttga aagactttcc agaaatcaaa atccatgctg
    10741 atcgggaccg gatgcatctt ttattgaatg gcgcagacgg gcagctagtt attccaaaac
    10801 tagcaaaaat atttgggatc caaaatttct ctccaagtat tcgtgtagaa aaagatgtcg
    10861 aagttttgaa acaagctgtt caagacatca tgaaagaaat ttatactggg aaagaaacat
    10921 ttaaaattat cgctaaaaga agcgatcacc aatttgaatt gacaagtaat gaattgaatc
    10981 aaacattagg taatgctgtt tttgatattt tcccgcatat ccaagttcaa atgcgtcaac
    11041 cagatattcc attacgtgta gagattcggc gtgatggcgc ttatctatcg tatgaaacga
    11101 tcaagggagc tggaggattg cccgtaggaa caagcggaaa aggaatgctt atgctttctg
    11161 gggggattga ttctcctgta gcagggtact ttgccatgaa gcggggagtg gaaattgaag
    11221 ctgttcattt tgctagtccg ccgtatacaa gtgaacaagc actgcaaaaa gcaaaagatc
    11281 taacagccaa actagcacct tatgtgggaa cgatccattt tatcgaagtg ccatttaccg
    11341 agattcaaga agaaatcaaa aagtcagtgc cacaaggata ttggatgaca attactcgtc
    11401 gaatgatgtt gcgtttgacc gatgaaattc gccgtattcg tcatggttta gtcattttga
    11461 atggtgaatc attaggtcaa gtggcttcac aaactttgca aagtatggtg gctatcaatg
    11521 aagtaacgaa tacgccaatc attcggcctg tagcgacaat ggataaactt gaaatcattg
    11581 aagtagcaga acagatcgat acatttaatc tttctatcca gccttttgaa gattgctgta
    11641 cgatttttgc tcctccacag ccaaaaacac gccctcaact ggaaaaggtc catcaatatg
    11701 aagagcgttt agcaatagat gaaatgatcc aacgagcact agctggcttg aagatggaag
    11761 agatacaagc agaatcgtct aatgaaaaaa ctgaagaatt ttcagaattg ctataagagg
    11821 ttatgaaaat agtgttttga ccagagtatt agagaaacaa aacgaaaaaa tagctccatg
    11881 tattttaccg tttttgaact aatgtcgaag attagctgat tgaataccat ttatcaagaa
    11941 gttatggaaa agccggttct tttttactaa ctgaaaaata aaattttatt tagagatcaa
    12001 gctacagttg ataaatgata taaaaaagag aatttcccat gaaatagcga ggttgtgaca
    12061 gaagtgggca gcttcaagaa ataagaagga atttacgaaa attgcttttc aaatttttgt
    12121 gaaatttcgg cttatttttc gaagaagtta cttctggaac accgtgtatc agaggttg
//
LOCUS       DAZKLD010000095        11498 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.095, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000095 DAZKLD010000000
VERSION     DAZKLD010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11498)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11498)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11498
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.095"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(84..689)
                     /locus_tag="ACG8KR_002031"
     CDS             complement(84..689)
                     /locus_tag="ACG8KR_002031"
                     /EC_number="2.1.1.172"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0052916 - 23S rRNA
                     (guanine(1835)-N(2))-methyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFQ0201388.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            885..1808
                     /gene="coaA"
                     /locus_tag="ACG8KR_002032"
     CDS             885..1808
                     /gene="coaA"
                     /locus_tag="ACG8KR_002032"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HFQ0201389.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            1867..3432
                     /gene="guaA"
                     /locus_tag="ACG8KR_002033"
     CDS             1867..3432
                     /gene="guaA"
                     /locus_tag="ACG8KR_002033"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HFQ0201390.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVNARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(3923..5554)
                     /locus_tag="ACG8KR_002034"
     CDS             complement(3923..5554)
                     /locus_tag="ACG8KR_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinogalactan endo-beta-1,4-galactanase"
                     /protein_id="HFQ0201391.1"
                     /translation="MKMGIWLRKAVLFAAVLGALGAVSKTVEASASEFIRGADISILS
                     DMEKSGAKYYEDDISKDALEILKNNGVNYVRLRLWQDPYNSNGESYGAGTNDLETTID
                     LAKRAKNLGLKVLLDFHYSDFWVDPGKQNLPKAWQGLTFEEMNTALYDYTKNVLSEMK
                     QLDVYPDMVQIGNELNSGMLWPYGKSWGEGGGEFDRLAAFLKSGIQAVRDTQPKTTPV
                     MLHLADGGDTGAFTWWFDEITSRGVSFDLIGVSYYPYWHGSMNELQTNMNAISQRYGK
                     EVIVVETAYGHTTANADTMPNAFGEAEAAAGGYEPTPTGQAEYLLDLADRIQAVPNNR
                     GAGFFYWEPLWYNGNVSWATQAGMNYLSVQSTMGNEWDNQAMFDFAGNALPSLRAFKQ
                     AGAQINLVKNASFESDGPTTSPSNWQTWFQQGTAKGTIKTEAGTFFGDYKLTFWQDQA
                     YEASAYQNVSGISKGTYTLSAYVMSGGEFDTNQLYIKNYGGAELNTAVVKTSEWTKIT
                     IKDIPITTGVCEIGVYSKASGNAWTNVDFVSLVKQ"
     gene            complement(5568..7241)
                     /locus_tag="ACG8KR_002035"
     CDS             complement(5568..7241)
                     /locus_tag="ACG8KR_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose transporter subunit IIBC"
                     /protein_id="HFQ0201392.1"
                     /translation="MDGIIKQIEKGKPFFEKISRNSYLGAVRDGFLTAMPAILFSSIF
                     ILIAAVPEIFGYQWPESVSTWLWKVYNYSMGVVGLLVSATTARCLADSMNRRLPKNKV
                     INTTSVMLASVVSFLLLSVSPIEGGFSTEYMGTKGLLASFVAAFLTVNLYKFCVLKDI
                     TIKMPKEVPGTISQTFRDIFPFSFSVFAAVLIDLGIRQIFQISFAEAIITVLQPLFTA
                     ADGYLGIAIIWGAMALFWFVGVHGPSIVEPAIAAIIYANVETNLQLFNSGEQASKVLT
                     VGLGNFVGTMGGTGATLVVPFIFLLFAKSKQLKAVGKASFIPVSFAVNEPLLFAAPMI
                     LNPYFFIPFLLTPVVNVCLFKFFVDVLGMNSFMYVLPWATPAPIGLVLGTGMGILAFV
                     LAAVLIVVDFLIYLPFCKAYDAVLVEKEAQKAEVIATEEGASADVREEVPVAASQVIS
                     DANKEINVLVLCAGAGTSGMLANALKKGASESKAPISAMAGAYGSHYEMLQDFDMVVL
                     APQVNSYYDDIKEDTDRLGIKLVATKGAEYIGLTRDPEKAVQFILNQFQ"
     gene            complement(7254..7571)
                     /locus_tag="ACG8KR_002036"
     CDS             complement(7254..7571)
                     /locus_tag="ACG8KR_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFQ0201393.1"
                     /translation="MNKEELQMLGFEIVAYSGDARSTLLKLLKEVRQGNFEHVDSALK
                     EADENLTLAHNSQTKILAEEASGKEMEMGFIFIHGQDHLMTTLLLRDLIQDFIVLYRQ
                     NQG"
     gene            complement(7596..8996)
                     /gene="lacG"
                     /locus_tag="ACG8KR_002037"
     CDS             complement(7596..8996)
                     /gene="lacG"
                     /locus_tag="ACG8KR_002037"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033920 - 6-phospho-beta-galactosidase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HFQ0201394.1"
                     /translation="MLKLPEDFIFGGATAAYQVEGATKEGGKGAVAWDDFLEEQGRFS
                     PDPASDFYHQYAKDIELCERFGVNGLRLSIAWSRIFPDGAGKPNPEGIAFYHRVFEEC
                     KKRNVTPFVTLHHFDTPKRLFDHGDFLNRETIEAFVSYAIFCFHEFKEVKVWSTFNEI
                     YPVATNQYLLGVFPPGIKYDFTKIVACLHNMMVAHARVVNYFKENELPGEIGVVHSLE
                     TKYAATDAPEDKHAAFLDDALSIRFLLDATYLGYYSTETLTALDEICEANQASYHFPE
                     EDFVELKKASTRNDYLGINHYQCHFVKAYDGENAIHHNGTGEKGTSVYKVKGIGERIY
                     KEGIPRTDWDWLIYPEGLYDLLLRIKSDYPHYNKIYITENGMGYKDQFEDGIIMDQPR
                     IDYLRVYLESLSKAITAGVNVKGYFLWSLMDLFSWTNGYNKRYGLFYVDFETQKRYPK
                     ESAYWYKLVSETKTII"
     gene            9233..10018
                     /locus_tag="ACG8KR_002038"
     CDS             9233..10018
                     /locus_tag="ACG8KR_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFQ0201395.1"
                     /translation="MLKKERLLTIVEMVNKKGILTVNEIINQLDVSDMTVRRDLDELE
                     KSGKLLRVHGGAQSLSYTLDQELSHTEKATLQIQEKKEIAATAASLIHEGETIFLGPG
                     TTIELLATHLLNKKIRIITNNYPVFDILAQADGPEILLIGGDFRKNTGAFVGPITNDN
                     LRRLKFTKAFISSNGVHNEAISTYSTEEGEAQQIALNNSRKKYLLVDNKKFNRDDFYV
                     FYNLHDFDLLITDRQISREVQEHYAQYVDLQIADSTENTTIGA"
     gene            10021..10713
                     /locus_tag="ACG8KR_002039"
     CDS             10021..10713
                     /locus_tag="ACG8KR_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFQ0201396.1"
                     /translation="MKGIIFDFNGTMFLDSHLHEAAWLHMIHDHTKDGLSDEDILKNI
                     HGRTNTEILKHFISEHLTASEIAVLSEEKESYYRMLCLQNENELQLTKGLEATLDQLT
                     DQQIPLTIATATVKENVAFYFDIFDLARWFDWDKIVYDDGSFPGKPQPDIFLKAADKL
                     SLAPADCLVIEDAYSGLLAAKRAGIGTIIAIDPFGKNKTVFEQAQLNKDGTIKDFTGF
                     WTEHLSATSVNS"
     gene            complement(10987..11358)
                     /locus_tag="ACG8KR_002040"
     CDS             complement(10987..11358)
                     /locus_tag="ACG8KR_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HFQ0201397.1"
                     /translation="MRDYVTDKTQEVINQSQSLNELSDKAKEEAVAIISSGISDYIDM
                     SGEINYGDKDKIVRMIKAFIKTFDVKADLKFNDTYLVGKLINILGTEKDTETFISSLS
                     MLFTDKHFSTQLGGEELVEIF"
BASE COUNT         3479 a         2475 c         2104 g         3440 t
ORIGIN      
        1 ttctcaacaa aaaaagaact gatacgtacc cgtacatgca cgcccagttc cactctttcg
       61 tcgctccact atttttcagc tagttattct ttcacacttt tgatgatata atatccttta
      121 tcttttgtga cgatttctgc attaccaaat gtttcttcca ttttcttttg cgcactcggc
      181 gctccttgtt tcttctggat cacaattgtc agtgtaccgc ccttcttcaa ccgaggatat
      241 gcgcctgtca aaatttcatg tacaactttt tttccagcac ggatcggcgg attgcttacg
      301 atagctgcat aggtttcttc atgaactgct tcatagatat tcgattgatg gatatcaaca
      361 tgttcgattt ggttacgctt tgcgtttcct tgcgctagac ttaccgctcg ttcattaatg
      421 tcgaccattt caacaaaccg ctgtgtcgca aatgccaaag ctaacccaat cggtccatac
      481 ccgcatccca catctaagat ctttccttca tcaggtaact cttcccaatc gaaagcatca
      541 atcaggacac gtgaaccata atccactgtt tcacgtgaaa atacaccaga atctgttaca
      601 aattgaaagg ttctgccttt taactcaaaa gcccattgtt caaaatcgtg cgccgtatct
      661 gggcgttcag aatagtaatg atttgtcatg tcttccatcc ttctcatttc tttcatgaca
      721 acagtctatc cttttatgta tccgtgcgca agtcaagaat aagactccgt ttctttctat
      781 tataaagatt atacaaaaag atcggcaatc tctcttaagt ggtggggttt gcgtttattt
      841 tgtgttaaaa tagttccatt gatgggagaa cgcaggaggc taatatgaat gatccaatga
      901 actactaccg tgtggcaaga gaagaatggc gagaatttta tcgtaatggg caagcacctt
      961 tgacacaaga tgaactagat aatatcaaaa gtttgaatga ccgcatttcc atgcaggatg
     1021 ttcgagatat ctatgtacct cttgctcatc tgatttatct ttatatgaaa gaatttgaat
     1081 ctttgttctt gagtaaaggt ttatttcttc ataaatacgt agaagtaccg ccttttatta
     1141 tcgggatcgc cggaagtgta gccgttggaa aaagtacaac agccagactg ctgcagacga
     1201 ttttgtctcg taccttcaaa cgaagaaacg ttcagctgat cacgactgac ggatttcttt
     1261 atccaaatcg tatattaaaa gagcgtggaa tcatggaccg taaaggattt cctgaaagtt
     1321 atgatatgga gatgctgatc gatttcttga accgtgtaaa atctggtcag gaagagataa
     1381 aagtacctgt ttactcacat gatgtgtatg atatcattcc agacaagtac gaattgatcc
     1441 agcagcctga tatcttgatc gttgagggga tcaatacgct tcaattacct gcgaatcagc
     1501 aaatctacgt cagcgacttt tttgattttt ctgtctatgt cgatgccgat cccgtgctga
     1561 tcgaaaaatg gtatttggat cgttttggtg cgttactgga tacggctttc caaaatccag
     1621 agaattatta ttatcgtttt gctatcggag atcgtgaaga agcctttaaa atggcaaaag
     1681 atgtatggaa aaatgtgaat ttgaaaaatt tacaggaata tatcttgcct acacgaggaa
     1741 gagccgatat aattctccat aaaactgaaa atcatatcat cgattcgatt ttcatgcgaa
     1801 aatattagca ttttaaaata gtttgactat tttgagataa aatttaaagg gataggggta
     1861 ataaatttga caaacgttgc cgatatgacg aacgttgaaa agattatcgt attagactac
     1921 ggtagtcagt ataaccaatt gatcacaaga cgtattcgtg atcttggagt cttttccgaa
     1981 ttattgagcc accgcattac tgcagaagaa gtaaaagcga tgaatccaaa gggaatcatt
     2041 ctttctggcg gaccaaacag tgtatatgat gagaatgcat ttgggatcga tcctgagatc
     2101 ttcaatttag gtattcctgt attagggatt tgctatggta tgcaattgat cacgtacaaa
     2161 cttggcggtt ccgtagagcc tgctaaaaac cgcgaatatg ggaaagcctt gttagaagta
     2221 acaagcgatc aagctgtctt gtttaaagaa acaccaaaac aacaaacagt atggatgagc
     2281 catggtgact tggtaactaa agttcctgaa ggctttgaaa cagtcggaac aagcaaagat
     2341 tgtccgattg cttctatcca agacacaaca cgtaattttt atggtatcca attccatgca
     2401 gaagtacgtc attccgaata cggaatcgaa ttgctgcgcc actttgcctt tgatgtttgt
     2461 cactgtgaag gtaactggac aatggataac tttatcgata tgcaagtaga aaaaatccgt
     2521 gaacaagttg gtgacaaaaa agtcttgctt ggtctttcag gtggcgttga ttctagtgtc
     2581 gttggggttc ttttacaaaa agcgatcggt gaccaattga catctatctt tgttgaccac
     2641 ggtctgctac gcaaaggcga agcggaacaa gtaatggaaa gtctaggcgg aaaattcggc
     2701 ttgaacatca tcaaagtcaa tgcaagagat cgcttcttag acaagttgaa aggtgtatct
     2761 gacccagaac aaaaacgtaa aatcattggg aatgaattcg tatatgtttt tgatgacgaa
     2821 gcaacaaaac ttgctggtga agaaggcgtc tctttcctag cacaaggaac gttgtatact
     2881 gacgttatcg aatctggtac agaaacagca cagacgatca aatctcacca taatgtaggt
     2941 ggactgccag aagatatgca atttgaattg atcgaaccat taaacacatt attcaaagac
     3001 gaagtccgtg aattagggac tcagttaggc atgcctgatt caatcgtttg gcgtcagcca
     3061 ttccctggtc ctggtcttgg tattcgtgtt cttggcgaaa tcactgaaga caagctagaa
     3121 atcgtacgcg aatctgatgc tatcttgcgt gaagaaatcg cagctgctgg acttgaccgt
     3181 gatatctggc aatatttcac tgttcttcca gggatccgct ctgtcggtgt tatgggtgac
     3241 ggacgtactt acgactacac agtcggtatc cgtgctgtaa cttcaatcga cggaatgacc
     3301 gctgactttg cccgtatccc atgggaagtc ttgcaaaaaa tctctgtacg tatcgtaaac
     3361 gaagtagccc acgtcaaccg tatcgtgtat gacatcactt ctaaaccacc agcaacagtg
     3421 gagtgggaat aaagagccaa cataatatgg agggggaatc cctataaatc aacattcttc
     3481 aatagctaaa atgcttgatt ttcattaaaa ataagatggt tccctaccaa aagagactat
     3541 aatataagtt aatttgtttc attcttatca taaaaagaag ttctcgaaaa acgaactgtt
     3601 ttccgagaac ttttttctat ccatctaagt gctcaaatca tattcacgac ggaattctaa
     3661 ggttaccctt tcttaaacaa actttatgtt atctgcacaa ctttttgcaa ttttttcatt
     3721 gcttcttacg aaatcgttga actctcttta gcataataca aagtgtagat gaagtctggt
     3781 gctttactcg ttttggtaaa aatgactgga aatcgtttct ggttattttt ggcaaaggcg
     3841 aggtgcaggt tcgattcctg ccttctacgc taacgatgtg gctgaaggga acctccgatc
     3901 ttgaatcgaa acgatccgca gtctattgtt tcaccaatga aacaaaatca acgttcgtcc
     3961 aagcatttcc tgaagccttg ctataaaccc caatttcaca aacacctgtt gtaattggaa
     4021 tgtctttgat tgtaattttt gtccattcac ttgttttaac aaccgcagta tttagttccg
     4081 ctccaccata atttttgata tataactgat tagtatcaaa ctctccccca gacattacat
     4141 aggcactcaa tgtgtaagtt cctttgctta tgccactaac attttgataa gctgatgcct
     4201 cgtatgcttg atcctgccaa aaagttagtt tgtagtcccc aaaaaaagtg cccgcttccg
     4261 ttttgatcgt acccttagcg gttccttgtt gaaaccaggt ttgccaattg gaaggactgg
     4321 tcgttggtcc atcgctttcg aaacttgcat ttttaactag atttatttgc gcccctgctt
     4381 gtttaaacgc acgcaagctt ggcaaggcgt ttccagcaaa atcaaacatc gcttggttgt
     4441 cccattcgtt tcccatagtg gattgaacac tgagataatt catacctgct tgtgttgccc
     4501 aactcacatt tccgttgtac caaagaggtt cccaatagaa gaagcccgcc ccgcgattat
     4561 tcggtactgc ctgaatccga tcggcaagat ccagcaaata ctctgcttgt cctgtcggtg
     4621 ttggttcata tccccccgca gctgcttcgg cttcaccaaa agcatttggc atagtatcgg
     4681 cattcgctgt tgtatgccca taagctgttt caactacgat cacttcttta ccataccgtt
     4741 ggctgatcgc attcatattg gtttgaagtt cattcattga accgtgccaa tacggataat
     4801 acgacactcc aattaaatca aatgacacac ctcgactcgt gatttcatca aaccaccaag
     4861 taaatgctcc tgtatcgcca ccatcagcta agtggagcat tactggagtt gttttaggtt
     4921 gagtgtctcg aacagcttgg ataccagact tcaaaaaagc agccaatcga tcaaattctc
     4981 ccccgccttc tccccaagac ttgccataag gccagagcat tccgctattc agttcattgc
     5041 cgatctgaac catatcagga taaacgtcca gctgcttcat ttccgacaga acgttctttg
     5101 tatagtcgta caatgccgta ttcatttctt caaaggtcag accttgccat gctttaggaa
     5161 gattctgttt cccaggatca acccaaaaat cactgtagtg aaaatccagc agcaccttaa
     5221 gtcccagatt ttttgctcgt tttgcaaggt caatcgtcgt ttccagatca tttgtccctg
     5281 ctccataact ttctccatta ctattataag gatcctgcca caaacgcagt cgaacatagt
     5341 tgacaccatt attttttaaa atctctaagg catcttttga aatatcgtct tcataatatt
     5401 ttgcaccaga tttttccata tctgacagga tcgaaatatc tgctccgcga ataaattcgc
     5461 tggcagatgc ttcaacagtt tttgaaacag cccccaaagc ccctaacaca gctgcaaata
     5521 aaacagcttt acgcaaccaa attcccattt tcattatggc tcctccttta ttgaaattgg
     5581 ttcagaataa actgaacagc tttttcagga tctctcgtta atccgatata ttctgctcct
     5641 tttgtcgcaa ccagcttgat tcctaagcga tcggtgtctt ctttgatatc atcatagtaa
     5701 gaattgactt gcggtgctaa gaccaccata tcaaaatcct gcagcatttc gtaatgagaa
     5761 ccataagcac ccgccattgc actgatcggt gctttcgatt cagatgcacc tttttttaaa
     5821 gcattagcta acatcccact tgtccctgcg ccagcacaaa gtaccaacac attgatttct
     5881 ttatttgcat cgctgataac ttgactcgct gccacaggta cttcctcacg tacatcggca
     5941 gaagcacctt cctcggttgc gatcacctct gctttttgtg cttctttctc tactaagaca
     6001 gcatcgtatg ctttgcaaaa tggtagatag atcaggaagt caacgacgat tagaaccgct
     6061 gctagaacaa aagctaagat acccatgccc gttcctagaa ccaaaccgat cggtgctgga
     6121 gttgcccaag gcaaaacgta cataaagctg ttcatgccca atacatcgac aaaaaacttg
     6181 aacaggcaaa cgtttacaac aggtgttaac aaaaatggaa taaaaaaata agggttcaaa
     6241 atcatgggtg ccgcaaatag caatggttcg ttgacagcga aacttactgg aataaaggaa
     6301 gctttcccaa ctgccttcaa ctgcttagac ttagcaaaga gtaggaagat aaaaggaaca
     6361 accaatgttg ccccagttcc tcccatagtt cctacaaaat tccctaaacc caccgtcagt
     6421 actttagaag cttgttcacc ggaattgaac aattgcagat tggtttccac attggcatag
     6481 ataattgccg caattgccgg ctccacgatc gacggtccat gaacaccgac aaaccaaaac
     6541 agcgccatcg ctccccagat aatagcgatt cctaagtacc cgtcagctgc tgtaaatagc
     6601 ggctgcagca ccgtaatgat tgcttctgca aatgagattt ggaagatttg tcgaatgcct
     6661 aaatcaatta aaacggccgc aaatactgaa aaagagaaag gaaaaatatc tcggaaggtt
     6721 tgcgaaatcg ttccaggaac ttctttaggc attttaatgg taatatcttt cagcacacaa
     6781 aacttatata aattaactgt taaaaatgca gcgacaaagg aagccagtag tccctttgtc
     6841 cccatatatt ccgttgaaaa tccaccttca atcggagaga cgcttaacaa caggaaactt
     6901 accacagagg ccagcatcac agaggtggtg ttgattactt tgtttttcgg taagcgacga
     6961 ttcatcgaat cagccaagca gcgagcagtg gttgctgaaa cgagtaaccc tacaacaccc
     7021 atcgaataat tatagacttt ccatagccat gtggagacag attcaggcca ttggtaacca
     7081 aaaatctcag gaaccgcagc aatcaaaatg aaaatgcttg aaaataaaat agctggcatc
     7141 gctgttaaaa atccgtcacg aacggctcct aaataactat tacgtgagat tttttcaaaa
     7201 aatggtttcc ctttttcgat ttgtttaatg attccatcca tagcgttctt ctcctatcct
     7261 tgattctgtc tgtacagtac aataaagtct tgaatcagat ctctcagcaa taaagtggtc
     7321 attaaatgat cctgcccatg gataaaaatg aatcccatct ccatctcttt cccagatgct
     7381 tcttccgcta aaatttttgt ttgcgaatta tgtgcaaggg ttaaattttc gtcggcttct
     7441 ttcaacgccg aatcaacatg ttcaaagttt ccttgtctta cttctttcaa taatttcaat
     7501 aatgtactgc gagcgtcgcc tgaatatgca acgatctcaa aaccaagcat ttgtaattcc
     7561 tctttattca tcttttcttc ctccattatc acttattaaa taatcgtttt ggtttcactg
     7621 accaacttgt accagtaagc tgattctttt gggtaacgct tctgggtttc aaaatcaaca
     7681 taaaacaagc catacctttt gttataccca tttgtccatg agaaaagatc cataagcgac
     7741 cacaaaaaat accccttcac atttaccccg gctgttatcg ccttgcttaa ggattccaaa
     7801 tagactctca aatagtcgat tctcggctga tccataataa tgccatcttc aaactgatct
     7861 ttatacccca tcccgttctc agtgatatag attttgttgt aatgtgggta atcgctttta
     7921 attcgcagca acaagtcata tagaccttct ggataaatca gccagtccca atcagttcga
     7981 ggaattcctt ctttataaat acgttctccg attcccttca ctttataaac agaggtccct
     8041 ttttcacccg taccgttatg atggattgca ttttctccat cgtaagcttt gacaaaatgg
     8101 cattggtagt gattgatccc taagtagtca ttacgcgtgg atgctttttt caattcaacg
     8161 aaatcttctt caggaaaatg atagctggcc tgatttgctt cacatatctc atcaagagca
     8221 gtcaaggttt cagttgaata atagcctaaa taggtagcat ctaataagaa acggatcgac
     8281 aatgcatcgt ctaaaaaggc agcatgctta tcttccggcg cgtctgttgc cgcatatttc
     8341 gtctctaatg agtgaactac accaatttca cccggaagtt cgttttcttt gaaatagttc
     8401 actacacggg catgagcaac catcatatta tgaagacagg cgacgatctt tgtaaaatca
     8461 tatttgattc ctggtgggaa aacacctaac aaatattgat tggttgcaac tggatagatt
     8521 tcattaaagg tactccacac cttgacttct ttgaattcat gaaaacaaaa aatggcatag
     8581 gaaacaaatg cttctattgt ttctcgattc aaaaaatctc catggtcaaa caaacgttta
     8641 ggggtatcaa aatgatgcaa cgtcacaaac ggcgttacgt ttcttttctt acattcttcg
     8701 aacacacgat gataaaaagc gattccttct ggatttggtt tcccagctcc atcaggaaaa
     8761 atgcggctcc aagcaatgga taatcttaga ccatttacac caaaacgttc acacaattca
     8821 atgtcctttg catattgatg ataaaaatca cttgccggat cgggactgaa tcgcccttgt
     8881 tcctctaaaa agtcatccca agcaacagcg cctttgccac cttccttagt ggctccttca
     8941 acctgatagg ctgctgtggc tccgccaaaa ataaaatctt ctggtaattt aagcatgtac
     9001 atctctcctc aaatctataa tcagttttcg ctctttctaa ctgattgcat tgtaaacgca
     9061 tccaaaacat ttgtcaacaa aaacaaacac aaataatcat aaaaaccaga aaacagcaat
     9121 aaaatctatt gtaacaaaca taaaaaccac cctaaatgtt tacttttgtt cgaattagat
     9181 tgactgttgt ccgtttatat gatagattat ccttaaatta gaggtgaaga caatgttgaa
     9241 aaaagaaaga ttgcttacta tcgtagaaat ggtcaataaa aaaggaatcc ttaccgttaa
     9301 tgaaatcatc aatcaattag atgtttctga tatgaccgtt cgcagagatt tagatgaatt
     9361 agaaaaatcg ggcaagctgc tgcgtgtcca tggaggggct caaagtcttt cctacacttt
     9421 agatcaagaa ctttctcaca ctgaaaaagc cactctgcag attcaagaaa aaaaagaaat
     9481 tgctgccaca gcagcctcct tgattcacga gggagaaacg attttcttag gaccaggtac
     9541 aacaatcgaa ctattggcga cgcatttact gaacaagaaa attcggatca ttacaaataa
     9601 ttatccagtt tttgatattc ttgctcaagc agatggacca gaaattctac tgattggcgg
     9661 tgatttccgg aaaaataccg gcgcatttgt cggaccgatc accaacgaca atttaagaag
     9721 actaaagttt acaaaagcat ttatcagttc aaatggcgtt cacaacgaag cgatctctac
     9781 ttacagtacc gaagaaggag aagcacagca gatcgctcta aataattccc gtaagaaata
     9841 tctactcgtt gataataaaa aattcaatcg agatgatttt tatgtgtttt ataatttgca
     9901 tgatttcgat ctgctcatta cggatcgcca gatctcgcgg gaggttcaag agcactatgc
     9961 acaatatgtc gatctgcaaa ttgctgattc aaccgaaaat accactatag gagcgtaact
    10021 atgaaaggga ttatatttga tttcaacgga acaatgtttt tggactctca tctgcatgaa
    10081 gctgcttggc tgcatatgat acatgatcat acaaaagatg gtttatcaga cgaggatatt
    10141 ttaaaaaata ttcatggtcg gacgaatacg gagattttga aacatttcat ttcagagcat
    10201 ttgaccgcct ctgagatcgc cgtattgtca gaagaaaaag aaagctatta tcgtatgctt
    10261 tgcttgcaaa atgaaaacga gcttcagttg acaaaagggc ttgaagcgac tcttgaccag
    10321 ctgactgacc aacagattcc attaacgatt gccacggcta ctgtcaaaga gaatgttgct
    10381 ttttattttg atattttcga tttagcccgc tggtttgact gggacaagat tgtctatgac
    10441 gacggctctt tccctgggaa accgcaaccg gatatttttc ttaaagcggc agacaaactg
    10501 agcttagctc cggctgattg tcttgttatc gaagatgctt actcaggatt acttgctgcc
    10561 aaacgtgcgg ggatcggaac aattattgca attgatccat tcggtaaaaa caaaacagta
    10621 tttgaacagg cacagctaaa taaagatgga accatcaaag attttactgg tttttggaca
    10681 gagcacctgt ctgccacatc cgttaattct taaacgattc aactaactac caaagaaaag
    10741 caactgaaat gcacacctcc ttttataaaa aactgccagt gaaacgacat gaaagataca
    10801 ctgctatatc cttagtgaaa tatagcagtg tatctttgac tttttcattc ttttaaccat
    10861 atgacgcgtt catctctatc tataattaac attaaaaata gtgttttcac attatttctc
    10921 aaaccaatct taaaaatcat caggagtact tttttgatat acttttatta atgctgggcg
    10981 taatggttaa aagatttcca ctagttcctc tccaccaagc tgagttgaaa aatgcttatc
    11041 tgtaaacaac atagacaaag aggaaataaa cgtttccgta tctttctctg tcccaaggat
    11101 attaattaat tttcctacta ggtaagtatc attaaactta agatctgcct tcacatcaaa
    11161 agttttgata aaagctttta tcatgcgtac aattttgtct ttatcgccat aattgatttc
    11221 accactcata tcaatatagt cactaatccc tgaagaaata atggcaactg cttcttcttt
    11281 cgccttatca gataattcat ttaatgactg ggattgattg atgacttctt gagttttatc
    11341 tgttacgtaa tctctcatta tcctatcggc atactccgca aagtaatctt gaaaaaattc
    11401 ttctacaggg taaccaactt cgttttccca accaggagaa attcattatc agtacttatt
    11461 aaatctatta aaaaccaacc cgttctctga gtgttgat
//
LOCUS       DAZKLD010000096        11250 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.096, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000096 DAZKLD010000000
VERSION     DAZKLD010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11250)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11250)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11250
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.096"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(13..1242)
                     /gene="hflX"
                     /locus_tag="ACG8KR_002041"
     CDS             complement(13..1242)
                     /gene="hflX"
                     /locus_tag="ACG8KR_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HFQ0201398.1"
                     /translation="MEEKVIIVGVETEANQRYFSESMEELVQLTNTASGEVVFTITQK
                     RPQVDRQTIIGKGKLQELVQQADAHEADLIIFNHELTPRQSQLITDAVGLPVIDRVQL
                     ILDIFAMRARSKEGKLQVELAQLEYLLPRLVGQGKTLSRLGGGIGTRGPGETKLETDR
                     RHIRNKIFAVKKELKEVEAHRERNRQKRKNSEIFQIGLIGYTNAGKSTILNLLTQADT
                     YSKDQLFATLDPLTKRWRFAEGFEITVTDTVGFIQDLPTQLIDAFHSTLEESQNMDLL
                     LHVVDASSPDRILQEQTVLKLMDELNMKEMPILTVYNKADQIDPAMFTPTLFPNVLIS
                     TQTKEGKMALIEAIKRQLMELMTPYTLSVPSNEGQKLSELRRQTMVLDEAYMEETNEY
                     EVKGFAKTNSKWLRKNQ"
     gene            complement(1244..2161)
                     /gene="miaA"
                     /locus_tag="ACG8KR_002042"
     CDS             complement(1244..2161)
                     /gene="miaA"
                     /locus_tag="ACG8KR_002042"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HFQ0201399.1"
                     /translation="MEKVLVIVGPTAVGKTALSVELAQLFGGEIISGDSLQIYKNLDI
                     GTAKIKEEEMNNVPHHLIDVIEPTQRYSAADFQHSGRQLITEIANRGHLPIIAGGTGL
                     YIQSLLYDYQLGATEEANPEIRKKYETLAETIGSQALWNQLKEKDPAAAEKIHWNNQR
                     KVIRALEVLETTGHSITAPKEEPKQLYDYLMIGLDTKRSILYERINQRVDLMLAEGLV
                     AEARKVYELGEVQASQGIGYKEFFPYFEGKETLEEATEQVKLHSRRYAKRQLTWFRNR
                     LDAQWFDLISEPNQKSQIEQTIKKWLEAE"
     gene            complement(2165..2902)
                     /locus_tag="ACG8KR_002043"
     CDS             complement(2165..2902)
                     /locus_tag="ACG8KR_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFQ0201400.1"
                     /translation="MLQIYAHRGSSGTHPENTLPAFAEAVRVGADGIELDVHLSKDGY
                     LIVMHDEEVDRTTNGKGLIREKTLEELKKLNAGGKFSKEFPAAKVPELREVLGLLLQK
                     NYRGMLMIEIKTDQYEYPGIEEKLAETLKSRDWPFQHAYCSFHLDSLEKIALLEPEIQ
                     LDLLMSTSDKKIQLAAETPYIEGVHPKNTWLFEHPVQAADFPKAIRAWTINDEEEMAK
                     CQELKIDGIITDFPEKALRWKQSTKSR"
     gene            complement(2993..3520)
                     /locus_tag="ACG8KR_002044"
     CDS             complement(2993..3520)
                     /locus_tag="ACG8KR_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0201401.1"
                     /translation="MIVGITGLIGVLTGAVVVYIGMILQMRHLENKEQRRRELELKVK
                     EIETLNQLNKKINEILQKRTTLMEEYVSFDAFDDCYITIDDFAYLQSFSAQNSFYLPN
                     YILDELFKNISHRKVILSPDETMKIGGYAYKGGRVILENFTDSLTEMINEKKAQLKTM
                     TRQPIGFFSKDDVWS"
     gene            3700..4293
                     /gene="clpP"
                     /locus_tag="ACG8KR_002045"
     CDS             3700..4293
                     /gene="clpP"
                     /locus_tag="ACG8KR_002045"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HFQ0201402.1"
                     /translation="MNLIPTVIEQSSRGERAYDIYSRLLKDRIIMLSGQVTDDLANSI
                     IAQLLFLDAQDSEKDIYLYINSPGGSVTAGMAIYDTMNFVKADVQTIVMGMAASMGSF
                     LLTAGTKGKRFALPNAEIMIHQPLGGAQGQATEIEIAARHILQTRERLNKILAERTGQ
                     PLEVIEKDTDRDNYMTAEQAKAYGLIDEVMENSSSLN"
     gene            complement(4397..4882)
                     /locus_tag="ACG8KR_002046"
     CDS             complement(4397..4882)
                     /locus_tag="ACG8KR_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HFQ0201403.1"
                     /translation="MERFIASNATVIGDVTLSEDVTIWYQAVLRGDSNWIKIGQRTNI
                     QDGTIIHVDHDAPVDIAENVTVGHQCMLHGCTIEKGALIGMGTTILNHAVIGENSLIG
                     AGSLVTEGKVIPPNVLAFGRPAKVIRPLTKEEIQKNKENIQHYVEIGRMHAQGKFKEQ
                     R"
     gene            complement(5035..5232)
                     /locus_tag="ACG8KR_002047"
     CDS             complement(5035..5232)
                     /locus_tag="ACG8KR_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0201404.1"
                     /translation="MEQFLNEKITEAKREVDRLTHVCQTKPFDSADFFENEFQIERLA
                     AQIEVYELILKKVIEENIDVQ"
     gene            complement(5327..7087)
                     /gene="efrB"
                     /locus_tag="ACG8KR_002048"
     CDS             complement(5327..7087)
                     /gene="efrB"
                     /locus_tag="ACG8KR_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HFQ0201405.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
     gene            complement(7084..8814)
                     /gene="efrA"
                     /locus_tag="ACG8KR_002049"
     CDS             complement(7084..8814)
                     /gene="efrA"
                     /locus_tag="ACG8KR_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HFQ0201406.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
     gene            9207..9419
                     /locus_tag="ACG8KR_002050"
     CDS             9207..9419
                     /locus_tag="ACG8KR_002050"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit epsilon"
                     /protein_id="HFQ0201407.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
     gene            9421..11100
                     /locus_tag="ACG8KR_002051"
     CDS             9421..11100
                     /locus_tag="ACG8KR_002051"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HFQ0201408.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
BASE COUNT         3263 a         2429 c         1971 g         3587 t
ORIGIN      
        1 aaaaataaaa ttttattggt tttttcttaa ccattttgaa ttggtttttg caaacccttt
       61 aacttcatat tcgtttgttt cttccatgta cgcttcgtcc aaaaccattg tttgtcttct
      121 caactcgctt aatttctgtc cttcgttgct tggcacagaa agtgtataag gggtcattag
      181 ttccatcagc tgacgtttga ttgcttcgat caacgccatc ttaccttctt tcgtctgagt
      241 agaaatcaat acatttggga atagcgtagg cgtgaacatc gctgggtcga tctggtctgc
      301 cttattgtaa acagtcagta tcggcatttc tttcatattc agttcatcca tcaattttaa
      361 gacagtctgc tcctgtaaaa tacgatctgg agaactagca tcgactacat gtagcaaaag
      421 atccatattt tgactttctt ctaatgtaga atggaaagca tcgatcagtt gcgtaggcag
      481 gtcttggatg aacccgacgg tatccgttac agtaatctca aatccttcag caaaacgcca
      541 tctcttagtc aatggatcta acgttgcaaa caattgatct ttcgaataag tatccgcttg
      601 tgtcaataga ttcaagatag ttgacttccc ggcattcgtg tagccaataa gtccaatctg
      661 aaaaatctcg ctattttttc ttttttggcg attgcgctct cgatgagctt ctacttcttt
      721 caattctttc ttaacagcaa aaattttatt ccgtatatgt cggcggtctg tttctaactt
      781 tgtttcacct ggccctcttg ttccgatccc accgccaaga cgagataatg ttttcccttg
      841 ccctactaaa cgaggcagca ggtattctaa ctgagcaagt tcaacctgca gcttcccttc
      901 tttcgaacga gcacgcatcg caaaaatatc taagataagc tgcacgcgat caattaccgg
      961 aagaccaaca gcatcggtaa tcagttggct ctgtctcgga gttagctcat gattaaagat
     1021 gatcaaatcc gcttcatgcg catccgcttg ttgaactaat tcttgtaatt tccctttacc
     1081 gataatcgtt tgccgatcca cttgtggtct cttttgtgta attgtgaata cgacttctcc
     1141 tgatgcggta ttcgtcagct gaactagctc ttccattgac tctgaaaagt aacgctggtt
     1201 tgcttcggtt tctaccccta cgatgatcac tttttcttcc attttattcc gcctccagcc
     1261 attttttgat tgtttgctca atctgacttt tttgatttgg ctcacttata agatcaaacc
     1321 actgtgcatc caaacggtta cgaaaccacg tcaactgacg tttcgcgtat cttctcgaat
     1381 gtagtttgac ttgttctgtt gcttcttcca atgtttcttt cccttcaaaa taagggaaaa
     1441 attctttata tccaatacct tgactggcct ggacttctcc aagctcatac accttacgcg
     1501 cttctgctac taaaccttct gctagcatca aatcgactct ttgattgatt cgttcataaa
     1561 gaatactacg cttcgtatct aatccaatca tgaggtaatc atacaattgc tttggctctt
     1621 ctttaggtgc cgtgatacta tgtcctgtcg tctctaaaac ttccagagct cttatcactt
     1681 tgcgttgatt gttccagtgg attttctcag cagctgccgg gtctttttct ttcaactgat
     1741 tccataatgc ttgggaacca attgtttcag ctaacgtttc atactttttt ctaatctcag
     1801 gattcgcttc ttctgtcgct cctaactgat aatcatatag aagggattgg atatagagac
     1861 ccgtaccacc agctatgata ggtaggtgcc ctcggttcgc aatctcggta atcaattgtc
     1921 gacctgaatg ctgaaaatca gcggctgagt aacgttgagt aggttcgatc acatcaatca
     1981 aatgatgcgg aacattattc atctcttctt ctttgatttt tgctgtaccg atatccagat
     2041 ttttatagat ttgcaatgaa tcaccactga tgatctctcc tccaaatagt tgtgcgagtt
     2101 ccacacttag cgctgtttta ccgactgctg tcggaccgac gatcactaat actttttcca
     2161 tattttacct acttttcgtt gattgtttcc accgaagagc tttctccgga aaatcagtta
     2221 ttattccatc tattttgagt tcttgacatt tggccatttc ttcttcatcg ttaatcgtcc
     2281 atgcacgaat tgcttttgga aaatcagctg cctggactgg atgttcgaac aaccaagtat
     2341 tttttggatg tactccttct atataagggg tttctgctgc tagttggatc tttttatcag
     2401 aagtactcat caataagtcc agctgtatct ccggttccag taatgcgatt ttttccaaag
     2461 aatccaaatg aaagctgcaa tatgcatgtt gaaaaggcca atctctactt tttaatgttt
     2521 cggctaattt ttcttcgata ccgggatatt catactgatc agttttaatt tcaatcatta
     2581 gcatccctcg gtaatttttt tggaggagca gtcctaacac ttctctcaat tcaggaactt
     2641 tagctgctgg aaattctttg ctgaatttgc cccctgcatt caattttttg agttcttcca
     2701 atgttttttc tcgaatcagc cctttaccgt tagtcgtccg gtccacttct tcgtcgtgca
     2761 taacgatcaa ataaccatct ttgcttagat ggacatctag ttcgatacca tctgcaccta
     2821 ctcgtacagc ttctgcaaat gcaggaagcg tattttctgg gtgggtgccg ctgcttccgc
     2881 gatgggcata gatttgtaac atctttcttc actccgttca gttctattgt atcattaaga
     2941 aaaaaagaaa gcattaccac gctctcatgt gcaacggcaa tgcttcaaaa tatcagctcc
     3001 aaacatcatc tttcgagaag aaaccaatcg gctggcgcgt catagtcttc aactgagctt
     3061 ttttttcatt gatcatttca gtcagactat ccgtaaaatt ctctaaaatc acgcgtccgc
     3121 ctttataagc atatccacca atcttcattg tttcatctgg agataaaatc acttttcgat
     3181 gggagatatt cttaaatagc tcgtctaaaa tataatttgg cagataaaaa ctattttgcg
     3241 ctgaaaatga ttggagatac gcaaaatcgt caatcgtgat ataacaatca tcaaatgcat
     3301 caaagctgac atattcttcc atcagcgttg tccgtttttg caagatctcg ttgatctttt
     3361 tatttaattg attcagcgtc tcgatttctt tgactttcaa ttccaattca cgtctacgct
     3421 gttctttatt ttccaagtga cgcatttgca gtatcatgcc gatatagaca actacagcac
     3481 ctgtcagcac tccgataagg ccagtaatac caactatcaa tctgttgttc ctccttgttt
     3541 tgtttctctg cttatcatac cataaagcgg gtttatgcga ctaatttgcc taagtttttt
     3601 atttgacctt ttttgaccaa aaatgtatac tacattttag gtattagaaa ctaaattatg
     3661 gtacactatt tctaaacgaa atcaaggagg aactctatta tgaatttaat tcctacagtc
     3721 attgaacaat cttcacgagg tgaaagagct tacgatatct actcacgttt attgaaagac
     3781 cgtatcatca tgttaagcgg ccaagtaaca gatgatcttg ctaattcgat catcgcccaa
     3841 ttgcttttct tagacgctca agattctgaa aaagatatct atctttatat caattctcct
     3901 ggtggttcag ttacagcagg tatggctatt tacgacacaa tgaactttgt caaagcagat
     3961 gtccaaacaa ttgttatggg tatggctgct tcaatgggaa gcttcttgct gacagctggt
     4021 acaaaaggta aacgatttgc tttaccaaat gctgagatca tgatccatca acctcttggc
     4081 ggcgctcaag ggcaagcaac tgaaatcgaa attgcggcac gccatatttt gcaaacacgc
     4141 gaacgcttaa acaaaatttt agctgaacgc actggacaac cgcttgaagt gattgaaaaa
     4201 gatacagatc gtgacaacta tatgacagct gaacaagcaa aagcatatgg attgatcgat
     4261 gaagtcatgg aaaacagttc ttcattaaat taatctgttt caaaggaagc ttttttaagc
     4321 tctcttatct aaaccaaata aaaaaggatg gtcaactcaa actgaccatc cttttttatt
     4381 ttatggatgc tactgcctat ctttgctcct tgaattttcc ttgcgcatgc atccgaccta
     4441 tctcgacata atgctggatg ttttctttgt ttttttgtat ttcttctttt gtcaaaggac
     4501 ggatcacttt agctgggcga ccaaaagcca agacattagg aggaataact tttccttctg
     4561 ttactaatga tccagcacca atcaaactat tctcaccaat aaccgcatga ttcaatatcg
     4621 tggtccccat cccgatcagc gccccttttt cgattgtaca gccatgtagc atgcactgat
     4681 gtcctaccgt cacattctcc gcaatatcta caggtgcatc atgatcgaca tggatgattg
     4741 taccatcttg gatattcgtt ctttgtccta tttttatcca attactgtcc ccgcgtaaaa
     4801 cagcttgata ccagatcgtc acatcttctg acaatgtcac atcaccaatc actgtggcat
     4861 ttgatgctat aaaccgttcc atatttattt ttctcctttc attttttgta gaaactaatc
     4921 ctattgaata aaggtatcat agcatatatt tatttcataa gaaaaaaaac aggtaaaacc
     4981 tgctcaggcc agatgtctat ttacagtatc tggtaactga cgttttacct gtttttattg
     5041 tacatctata ttttcttcta ttactttttt taaaattaat tcgtacactt ctatttgtgc
     5101 tgccaagcgt tcgatttgaa actcattttc aaaaaaatca gctgaatcaa aaggcttcgt
     5161 ctgacagacg tgcgttaaac gatcaacttc tcttttggct tcagtaattt tttcatttaa
     5221 aaactgttcc atctccgtgt cctcactttt cttgataact ttactttacc attatcaaga
     5281 atagagaaca aaaaataagt tttaaactgt tttattctta tatgacttat tctgcttcct
     5341 cagcaaattg gctgttatac agtttttcat aaaatccacc ttgtgccaat aaactctcat
     5401 gcgttccttt ttcaatgatc tgaccttgat ccatcactag aatcagatct gcatcacgga
     5461 ttgtcgataa acggtgagcg atgacgaaac ttgtccgtcc ctccatcacg cgatccattg
     5521 ctttttgaat caacgcttct aaacgggtgt ctactgaact tgttgcttca tctaaaatca
     5581 agattttagg atcggagacc actgctcggg caatcgtcag caactgtttt tgtcctaaag
     5641 acacattgtc tccctcagaa ttgatggcca tatcatatcc atctggcatc gttcggataa
     5701 aatgatctac gtttgctgtt ttcgcagcat caactacttc ataatctgtc gcatcaagct
     5761 ttccgaagcg gatattatct gcaatcgttc cttgataaag ccatgcatct tgcaagacca
     5821 taccaaatag cgaacgcaca tcactgcgac tcattgattt ggtgtcgatt ccatcaatcc
     5881 ttattgctcc tttgtctacg tcataaaaac gcatcaatag attgatcaat gtcgtcttgc
     5941 cggcaccggt cggaccgacc acagctacgg tttgaccagc tttcacttca aaattcaaat
     6001 cttggattaa tggtttcttc ggatcatatg caaaatcgac atgatcgaat gtcacatctc
     6061 ctaaaatcgt ttctggcagt gaagcatcta ctttgttttc tttttcttct ggctcatcta
     6121 aaatctcgaa tacacgtttc gttgccgctg atgcactttg caaaactgaa gacaattgtg
     6181 tcacgttccc cattggctga ctgatttgcc aaatatattg gatgaatgct tgtagttgac
     6241 cgacaacgat cgccccagta ataacataaa agcttcctag aacagccata ccaatataag
     6301 tagcataggc agttaattga actagaggca tcatcaagcc agaaatgaat gctgcacgaa
     6361 acccaaagta tttcaagctg tgattaactt ttttgaatcc ttccaatgtt tctttttcgc
     6421 gtccatatac tttcaataca ctaaaacctg tcatattttc ttggacgtag ccgtttagct
     6481 caccaagaga attctgcatg ctttgaaaat atttctgcga agcattgatc actcgttttg
     6541 agatccagat cgatgttgga atcatgatca tagagatcag cgccatcatc ggctggatat
     6601 aaaacatcat tgctgcagcc aacgtaattc ccaatatcgc atttacgata ccaataaaag
     6661 cttgttgtaa agcaccgctg actgcatcta catcattcgt cacacgagac aaaatatttc
     6721 cttgctggtt tttatcgaaa tatgaaaccg gaagacgatt gattttttct tctatgtctc
     6781 ttctcaaatc atgcatggca ctttgaacga catttgtcat caaaacacca gaaagtaatt
     6841 gcgaaataca ataccctatt ccaacaactg tgatccaaat gagacatttg aatatataag
     6901 aaaaattgat tgattcgcca ttggcaatat tacggctgat ttctgtagta ggaagtcctg
     6961 taacatatgg caaagccacg ttgaaagcca ctgtcagaac tgtaaatagg atcactaaaa
     7021 gaaaagtctc acgatacggt ctgatatatc gagctaaacg ttttaaggaa gaaaaggttt
     7081 ttttcatgct aattcctcct ttgataattg agaagcggca atatcataat aaatcgggca
     7141 tttttccaac aactcccgat gcgtccccat accgacgact tctccttcat ttaatacgat
     7201 gatcttatct gcatgcatga tcgttcccac acgttgtgct acgatcaata cagtagattc
     7261 tgtcgtttct tttttcagac gagcacgtaa cttcgcatcc gtttgataat ctaatgcaga
     7321 aaagctgtca tcaaaaatat agatttccgg acggcgtatg atggctctgg ctattgccaa
     7381 tcgttgtttt tgtccacctg aaaaattgga accaccttct gataaatgtt cattaaatcc
     7441 atctggtttt cgagaaatga attctgaagc ttgagcaatc tcagctgcac gttccatttc
     7501 ttcttgcgta gcatcttctt tcccgtatcg caaattatct gcaatcgttc cagtaaatag
     7561 cagcgctttt tgcggaataa acccaatctt atctcgcaac ttgcttagcc gataatctct
     7621 cacgtctact ccatcgatca agatctttcc ttcactcaca tcataaaagc gagggatcaa
     7681 ttggatcaac gtagattttc cgctaccggt acttccaata aaagcaacgg tctctcctgg
     7741 agaggctgta aaactgacat ttcgtatcac tggctcttgc gaatggcctg gataagcaaa
     7801 tgttacattt tgaaattcga tatacccttt agtttttgta tcagatatac catttggatc
     7861 ttctacaatc gaaggtttgg catctagggc ttcttggata cgtccagctg aaacagctgc
     7921 tcttggatac atcatgaaca cactagcaaa taacatgaat gagaacaagg catggaatat
     7981 atactcgata aacgcaatca aatctcctac acgaagctcg cctgcatcga tttgcactgc
     8041 cccgctccag atgatcaata ccattacgat attaaataag aaataaaaac ccggttgtgc
     8101 aacagccatc aaacggaata agctttttga actagaagca taattctcat tgactacagc
     8161 aaaacgtttt tcttcaaact tttcattgac gaatgcacga atcacacgca atcctgaaag
     8221 attttcccgc aagatactgt tgattttatc taagtttttt tgttgctttt tagataacgg
     8281 ttcagacacc ttagcaatcg ctacgactgc caataatagt aaaggtaatg cacctactac
     8341 atacaaactg agtgtcacac ttgtacgcat gaccatgtac aaactgacaa tgaacatcat
     8401 tggtgccata aaaccgatac gtaaaatatt ttgcaggaac aacatgatct gataagcatc
     8461 gtttgttgta cgagtgatca atgaagatac gcctagattt tcgtattcct gatgcgaata
     8521 actttggatc ttttcgaaca aatcatctcg aatatctgct acgatattag tcgtaatcct
     8581 cgaaccaaaa taacctaaca agatattcaa aataataccg attacagtaa taacgatcat
     8641 aaatatccct tgttgcttca cataatcaaa atcatcattg cggattccta catcgatcat
     8701 acgtgctaaa atcgtaggaa gtcctaattc aatcaaaata aaaccaaata cgcatatgaa
     8761 attgaaaaac aagaatttct tataacgcat ggtataatgc cacattagtt tcactttctc
     8821 atctcctttt aatcttttgt tacattttca ttactaaaaa tgatagcaca acgagcctat
     8881 taatagaaca cttctctcat aaaaaagtca ttatgattat agcgggatga taaacattga
     8941 ttttttcttt caatctagtt gatttctata cttatttgct gttttatgct agaataacaa
     9001 ttaattgttg tatatttttt atacagaagg tacggttgaa aaaatatccg aacagataag
     9061 caaaaacgag gcttgtctct ttgactcgaa tccagtattc tagtcaaagc atatgaaaaa
     9121 ccaggtggct tgtcccctcg ttttgtgttg tctaaaaaaa tcaactagct aaatcactgc
     9181 tttacgcaga taaaggagac aaaacaatga tctataaagt ttattatcaa gaaacaaaag
     9241 tacgcaaccc taaacgggaa gaaacaaaat ctctttacat tgaggcaaaa agcgatgtag
     9301 acgctcgcca acgtgtggaa gaaaataccc catacaatat tgaattcgtt caacttctag
     9361 aaggaagtca tctagagtac gaaaaagaaa atgctgattt ctcattagtg gagttttaat
     9421 atgaaacttt ccatcaaaaa taacgagaca ggcgtctttg ctattggtgg attaggtgaa
     9481 atcgggaaaa atacgtatgg ggtacaattc caagatgaaa tcattttgat tgatgccggg
     9541 atcaaattcc cggaagacga tttacttgga attgactatg tcattcccga ctacagctac
     9601 atcatacaaa acttacacaa agtaaaagcc ttggtgatta ctcacgggca cgaagaccac
     9661 atcggcggaa taccctactt gctgaaacaa gcaaatattc ctatctatgc tggtcctctc
     9721 gcattagcgt tgatcacaaa caaattagat gagcatggct tgcttcgaga tgcagagctc
     9781 catgagatca atgaagatac gatcatccgt tttagaaaga cttctgtcag ctttttcaga
     9841 acgacccata gtatcccaga tgcattaggg atcgtggtga aaacaccttc tggaaatatc
     9901 gtagcgactg gagacttcaa attcgatttc actcctgttg gcgaaccagc aaacttgcat
     9961 aaaatggctc gtatcgggga agaaggagtc ttgtgccttt tatctgatag tacaaatgct
    10021 gagattccaa catttactaa atcagaaaaa acgattgggc attctatttt gaagatcttt
    10081 tcaaaaatcg aaggacgtat catttttgcc agctttgctt ctaacatctt ccgtttgcag
    10141 caagcagcag atgccgctgt ggcaactggt cgtaaaatcg ctgttttcgg acgaagcatg
    10201 gaaaatgcca tcgtcaacgg tcaacgttta ggatatatca aagttccaga cggcacattt
    10261 gtagatgcac acgaaatcaa tcgcttgcct gccaatgaaa cgatgatcct atgtacaggt
    10321 tctcaaggag aagcgatggc cgcgttgagc cgcatcgcga atggtactca ccgtcaaatc
    10381 caaatccagc cagacgatac agtgatcttt tcaagttcac ctattcctgg taatacaaca
    10441 agtgtcaatc atttgatcaa tttgctgtct gaagctggtg cagaagtgat tcacggaaaa
    10501 atcaataaca tccatacttc cggtcacggt ggacaagaag aacaaaaatt aatgcttcgt
    10561 ttgatgaagc caaaatattt tattcctgtc cacggagaat tccgcatgtt gaaagtacat
    10621 gcttctctag cacaagatgt aggtgttcct gcagaaaact gcttcatcat ggaaaatggc
    10681 gatgttttag cgttaactag cgattctgcg cgtcctgctg ggcattttgt ggctggagat
    10741 gtgtatatcg atggtagcgg gatcggtgat attggtaatg tcgttttacg tgaccgtcac
    10801 ctgctttcag aagaaggtct tgtattagct gtcgcgacag tggatatcaa gaaaaaagaa
    10861 atcttggctg gtccagatat tctctctcgt ggatttgtct acatgcgaga atcaggagaa
    10921 atgatcaatg aagctcagcg aatcttattc cacgctttaa gagaatgttt gaatggacct
    10981 gactgttcag aatataaatt gaaagaagcg atgactggcg cgcttcagcc tttcttattc
    11041 gaacgtacag aaagacatcc aatgattttg ccaatggtaa tgacaccaga tgaagaataa
    11101 ataagaggtt atgaaatcag caccttgtcc tgaatataag agaaaacaaa tgaaaaacat
    11161 gagaagccat ttttgtttgt tcttattcct tgatacaagg tggctctttc atagcctctt
    11221 aaatttattt actttttcat acaaaaaaat
//
LOCUS       DAZKLD010000097        11248 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.097, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000097 DAZKLD010000000
VERSION     DAZKLD010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11248)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11248)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11248
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.097"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(4..2013)
                     /gene="metG"
                     /locus_tag="ACG8KR_002052"
     CDS             complement(4..2013)
                     /gene="metG"
                     /locus_tag="ACG8KR_002052"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HFQ0201409.1"
                     /translation="MAEKETFYITTPIYYPSGKLHIGNSYTTIACDAIARYKRLMGFD
                     VFYLTGVDEHGQKIETKAEELGVKPQEYVDKMAADVKKLWKTLDISYDKFIRTTDDYH
                     KAAVQKIFDRLLEQGDIYLGEYEGWYSVSDEEFFTETQLAEVYKDDEGNVIGGKAPSG
                     HEVELVKEESYFFRMSKYADRLLEYYEEHPEFIQPESRKNEMINNFIKPGLEDLAVSR
                     TTFTWGVPVKSDPKHVVYVWIDALSNYITALGYGSEDESLFEKYWPADVQMVGKEIVR
                     FHTIYWPIMLMALDLPLPKKVFGHGWLLMKDGKMSKSKGNVVYPEMLVERYGLDALRY
                     YLLRAVPFGSDGVFTPEDFVSRVNYDLANDLGNLLNRTIAMINKYCDGLVPDYASLVT
                     PFDSELSTTAANVVGRYHDAMEKMEFNTALAEIWVLISRANKYIDETEPWVLAKDEEK
                     KAELNSVMIHLAESLRIAAILLQPIMTETPEKIFNQLGLDSETMNLEGLHFGEFPSGT
                     KVVAKGTPIFPRLDMEEEVAFIQEKMSEGTQTNEDTVKWDPEETELVSTKEKQIKFDV
                     FEKVELKVAEVINCQKVEGADKLLQFRLDAGDSQDRQILSGIAEFYPDPSELIGKKVV
                     IVANLKPRKMRGQISQGMILSAEAPDGSLQVIEAPKSMPNGSEIA"
     gene            2281..2559
                     /locus_tag="ACG8KR_002053"
     CDS             2281..2559
                     /locus_tag="ACG8KR_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0201410.1"
                     /translation="MQKKHLFFTLSIAFLSLAHLIFSYFYIRMYGYFNLHGHLNSFMT
                     AAWILRFIIDVYIVICGFFAIREERYKVLPFYLLFFLFNLILPFIFHI"
     gene            2656..3477
                     /locus_tag="ACG8KR_002054"
     CDS             2656..3477
                     /locus_tag="ACG8KR_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HFQ0201411.1"
                     /translation="MNRFASKEEEKLYYEHKASEEEFLNWYQKQERPEYDKPSLTVDI
                     VLMCYNKEEDQLKVLLIQRKGHPYRNSWALPGGFVQKDESTGESVLRETKEETGVVIS
                     KENIEQLHTFSTPNRDPRGWVVTVSYLAFIGEEPLIAGDDAKEVRWFTLERHSNKIHL
                     SSGEVAISLDLVTGESSGKDTLAFDHSQIILKAFNRVVNKMEHEPQVLQVLGKDFTIT
                     EARKVFAKFLGIDYKTIDHSNFKKAMLQYFDEIGERPVGIGRPSKIYQLKPGHHE"
     gene            complement(3762..4772)
                     /locus_tag="ACG8KR_002055"
     CDS             complement(3762..4772)
                     /locus_tag="ACG8KR_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease TnpB family protein"
                     /protein_id="HFQ0201412.1"
                     /translation="MLKAFKFRMYPTEEQKQQLIRTFGCARFTYNHLLKKRQKSWQQT
                     GVANFSLTPATLKKEYPFLKEVDSLALANAQLNLDRAFRNYFKGRASFPKLKTKKSMW
                     QSYTTNNQTRTIYLENGYLKLPKQKELIKINQHRPVEGSIRSATISARYNEEFYVALL
                     CDVSPVKKESLAKWIGIAYHPKTLIQTSRPLEVTLPKFHQTEEKLQHAQRKLNVKVRS
                     AHHRKIRLDQASNYQKQKRKVMDLYLKQKNQREDYLEQLSGKLVKQYDYLFVESFPKE
                     EAHADFSIHDWHKLITKLRYKSQWYNKKFLLINTDGAEESNSVRKSQVVEKMGRHSVI
                     KG"
     gene            complement(4784..5737)
                     /locus_tag="ACG8KR_002056"
     CDS             complement(4784..5737)
                     /locus_tag="ACG8KR_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0201413.1"
                     /translation="MPNKLLEVKGLKQYFNVGKKNEVHAVNDISFHIYEGETFGLVGE
                     SGSGKSTTGRTIIRLNEPTDGEILFDGQDITKIKDKKGLTKFRHDVQMIFQDPYASLN
                     PRMKVRDIIAEGIDVNGLAKSSEERAKKVDDLLRTVGLNPSHGTRYPHEFSGGQRQRI
                     GIARALAVKPRFIICDEPISALDVSIQAQVVNLLQDLQKEHQLTYLFIAHDLSMVKHI
                     SDRIGVMHNGLLLEMGTSDEIYNHGVHPYTESLLSAIPLPDPDHERKRRRIKYQPEPD
                     DGQVRQLREIAPEHFVYATEQEVPYYAKKLKRQKESLLVAN"
     gene            complement(5727..6797)
                     /locus_tag="ACG8KR_002057"
     CDS             complement(5727..6797)
                     /locus_tag="ACG8KR_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0201414.1"
                     /translation="MTKILEVKDLEISFDTYAGKVRAIRGVNFHLNKGETLAIVGESG
                     SGKSVTTRSIMRLLSSNANIDAGQILFKGNDIVGKSEREMQKIRGKEIAMIFQDPMTS
                     LDPTMPIGKQVAESLRKHNKVSKKEGQKAALELLKLVGIPEAEKRINSYPHQFSGGQR
                     QRIVIAIALVCYPEILIADEPTTALDVTIQAQILELLKEIQTKIDTSIIFITHDLGVV
                     ANVADRVAVMYGGRIVEVGTSEEIFYNPQHPYTWGLLGSMPTLDSANDRLYAIPGSPP
                     DLLNPPKGDAFYPRNEFAMKIDAEQEPPFFELSKTHKAATWLLAPQAPKVTPPEEIQR
                     RWAIFKEKQKHTYGQGGIADAK"
     gene            complement(6803..7846)
                     /locus_tag="ACG8KR_002058"
     CDS             complement(6803..7846)
                     /locus_tag="ACG8KR_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0201415.1"
                     /translation="MEMIPKRYETVADIPADEFLPLQKNTEEEREQITAPSLNFIQDS
                     WRRLKKNKAAVISMVLLLVIILISVITIFVSPHDPTAQNVAYINLPPRIPGINIDGLN
                     GKAMVGGELVDKYAQANVPSNVNFFLGTDGLGRDVLSRLFMGMRISLLIAFIAALLDV
                     TIGVAYGLVSGLLGGRVDNVMQRFLEILSGIPNLVVMILMLVVFEPGIFSIVAAMAIT
                     NWIPMARIVRAQTMKLKDQEYVLAGLTLGESRMKIAFKHILPNISSVIIIQMMFSIPS
                     AIFFEAFLSFIGLGLTPPSASLGTMLSEGYKTFLYLPYLLWIPAVTLSVIMIGFNLLA
                     DGLRDAFDPKMKE"
     gene            complement(7847..8788)
                     /gene="opp3b"
                     /locus_tag="ACG8KR_002059"
     CDS             complement(7847..8788)
                     /gene="opp3b"
                     /locus_tag="ACG8KR_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease"
                     /protein_id="HFQ0201416.1"
                     /translation="MNSYIKYVLKRVFFMIITLWLIATITFFLMQLLPGTPYTNQERL
                     SPETIEMLNKQVGLDKPVIVQYGIYLSNLLQGDFGISFQFKNQPVAHLLAGRIGPSLQ
                     LGLQAIIFGTVLGTILGTISAMKQNTWADTSSTLVAILGRSIPNFVFAVLLQYIFAIK
                     LQVLPIAKWDGFVYTILPTIALAMSPLADSARFIRTEMVEVLHSDYVELAKAKGLSRW
                     EIAFKHGLRNSLIPLMTLLGPLAVALMTGSLVVENIFAIPGIGEQFVKSITTNDYPTI
                     MAVTILYSFMLIFVILIVDLLYGLVDPRIRVSEGSRG"
     gene            8872..9219
                     /locus_tag="ACG8KR_002060"
     CDS             8872..9219
                     /locus_tag="ACG8KR_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HFQ0201417.1"
                     /translation="MKKKSIPYAVAFLLILVILIKNLINHSFTLIQLSNDLFLWSLPF
                     LIIGGFLWVFSSGFFDHFQRSVHLARTRNRKKKPEFSSLSSASYGMYSFWLIIAGILI
                     ALSAIFMLFSLLG"
     gene            complement(9289..10950)
                     /locus_tag="ACG8KR_002061"
     CDS             complement(9289..10950)
                     /locus_tag="ACG8KR_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HFQ0201418.1"
                     /translation="MKKKFTFGLVAVCGLVLAGCYGGSSATSSSSAKSGSADGGGVFN
                     LVVPQEMPTADLSVATDTISFTALNNVYEGIYRLDEDSKPQPAGASELAEVSEDGLTY
                     KIKLREDAKWSNGDPVTAADYVYGWQRTVDPATASEYAYLYAPVENAEDITAGKKDKS
                     ELGIKAVGDYELEIKLTKQTPYFQYLLAFPSFFPQKQSVVEENGDAYASASDKAVYNG
                     PFTLEDFDGPGTDTEWTYKKNDHYWDKDTVKLSEIKVSVVKESSTALNLFKDGQADDV
                     ILSGELAQQNANDPAYTSVKEARTSYIEFNQREKNSPFNNVNLRKAISYSIDREALVK
                     QVLGDGSVVSTGLIPSDMVKSPTTNEDFAKEAGKLVSYDKDKAKEYWEKAKKELGVDS
                     LEFDLMASDDDSSKKVIEYVQNSIQENLDGVTVKPTPVPFSVRLDRSTSGDFDAVLGG
                     WAADYADPSSFTDLFVTGNSYNRGRWSNAEYDEAVKAASSTDAGDEQARWDDFQKAIK
                     IISDDMGVVPVYQKAEGHLVNEKIKGIVHHAAGASWDYKWTYVEE"
     gene            11129..11248
                     /locus_tag="ACG8KR_002062"
     CDS             11129..11248
                     /locus_tag="ACG8KR_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HFQ0201419.1"
                     /translation="MVFQKPLNNFRYIVYYCHTKIKKNKQKETVTKVLSQSLI"
BASE COUNT         3173 a         2287 c         2046 g         3742 t
ORIGIN      
        1 tttctatgcg atttctgagc catttggcat ggattttggt gcttcgatta cttgtaaaga
       61 gccatctggt gcttctgcag aaaggatcat tccttgactg atttgtccac gcatctttcg
      121 tggtttcaga tttgctacaa tgacgacttt tttcccgatc aattcgcttg gatcaggata
      181 aaattcagcg attcctgaca agatttgacg gtcttggctg tctccagcat ctagtcggaa
      241 ttgcagcaac ttatctgccc cttctacttt ttgacagttg atgacttctg ctacttttag
      301 ttctactttt tcaaatacat caaatttgat ttgtttttct ttcgttgaaa caagttctgt
      361 ttcttctgga tcccatttga ctgtatcttc gtttgtttga gtaccttcag acattttttc
      421 ttgaataaaa gcaacttctt cttccatatc taaacgtggg aagattggtg taccttttgc
      481 aactactttt gttcctgaag gaaattcgcc aaagtgtaat ccttcaagat tcattgtttc
      541 agagtccaat cctagttgat tgaagatctt ctctggcgtt tctgtcatga ttggctgaag
      601 taatatcgct gcgatacgca aagattcagc taaatggatc atgacactat tcaattcagc
      661 tttcttttct tcgtcttttg ctaataccca tggttctgtt tcatcaatgt atttgtttgc
      721 acgagaaatc agcacccaga tttcagccaa tgctgtattg aattccattt tttccattgc
      781 atcatggtaa cgtccgacaa cgttagcagc cgtcgtagac agttcactat caaatggagt
      841 gacaagtgag gcatagtcag gaacgagtcc atcacaatat ttgttgatca tagcaatcgt
      901 tcgattcaat aagttcccta gatcatttgc cagatcataa ttgacacggg aaacaaaatc
      961 ttctggcgta aagacaccat ctgacccaaa cggaacagca cgaagcaaat aataacggag
     1021 tgcatctaaa ccataacgtt ccacaagcat ttcaggataa acgacatttc cttttgattt
     1081 agacattttt ccgtctttca tcaatagcca gccatgccca aatacttttt taggtagagg
     1141 caaatcaagg gccatcagca tgattggcca atagatcgta tgaaagcgaa cgatttcttt
     1201 accgaccatc tgaacatctg caggccagta tttttcgaat agactttcat cttcggagcc
     1261 atatcctagt gcagtaatat aattggataa tgcatcgatc catacataca ccacatgttt
     1321 cggatcactc ttgactggta ctccccaagt aaaagttgtt cgtgaaactg caagatcctc
     1381 taatcctggt ttgataaagt tgttgatcat ttcgttttta cgagattctg gctggatgaa
     1441 ttcgggatgt tcttcatagt attccaatag acggtctgcg tatttgctca tgcggaagaa
     1501 gtatgattct tcttttacca attctacttc gtgtccactt ggcgctttac caccgatcac
     1561 gttaccttcg tcatctttgt acacttctgc aagttgtgtt tctgtaaaga attcttcgtc
     1621 agaaacagag taccagcctt catattcacc taagtaaata tctccctgtt ctaataaacg
     1681 atcaaatatt ttttgaacag ctgctttatg gtaatcatct gtcgtgcgaa tgaatttatc
     1741 ataactgata tcaagcgttt tccataattt cttgacatca gccgccattt tatctacata
     1801 ttcttgcggt tttacaccta gttcttctgc ttttgtttcg attttttgac catgttcatc
     1861 cactccagtg agataaaaca catcaaagcc catcaaccgt ttataacgag caattgcgtc
     1921 acatgcaatc gtagtatacg agttgccgat atgaagtttg ccgcttggat aatagattgg
     1981 cgtagtgata taaaaagttt ccttttctgc cacggggctc ttcctccttt atcgcatgat
     2041 gcgaattcgt ttccttgcta gtataccata aaaaaagcat gctgttagct ttttttcaaa
     2101 atgaaaaaga ctggataaac gacttctatc tacgttcaat ctatccttta aaaggatgaa
     2161 taggttgaat aaatgcgggt ttttgttttg tttcatatct actaaaaaac ggctttttat
     2221 ttctcccctc acacgtggta taataaagac tatctatctt agtaagggac tggaacaatc
     2281 atgcagaaga aacatctttt ttttacactt agtattgctt ttctttcatt agcacattta
     2341 atattcagct atttttatat tcgtatgtat ggttatttta acctccatgg gcatttgaac
     2401 agttttatga cagcagcttg gattttgcgt tttatcattg acgtatatat cgttatttgt
     2461 ggcttctttg ccattcgtga agagcggtat aaagtattgc cgttttatct gcttttcttt
     2521 ttattcaatc tcatacttcc atttattttt catatttaaa tgataaccta gatttttcaa
     2581 gtaaatcctt ggtttttttt cagagtctgc tacaataagg taaaaagtac ttttactgtc
     2641 aatggaggtg gtcagatgaa ccgttttgct tctaaagaag aagaaaaatt atattatgaa
     2701 cataaagcaa gtgaagaaga atttcttaac tggtatcaaa aacaggaacg tccagaatat
     2761 gacaagccct ctttgactgt cgatatcgta ctgatgtgct ataacaaaga agaagatcag
     2821 ctgaaagttt tgttgatcca aagaaaaggg catccttatc gaaattcatg ggcattgcct
     2881 ggtggctttg tccaaaagga tgagtcaact ggagaaagcg tcttaagaga aacaaaggaa
     2941 gaaactggtg tggttatttc aaaagaaaat attgagcaac tgcatacatt cagtacgcct
     3001 aatcgagatc cgcgaggctg ggtcgtcaca gtcagctact tagcctttat tggagaagaa
     3061 ccgctaatcg ccggagatga tgccaaggaa gtacgctggt tcacactaga acgtcatagt
     3121 aacaagatcc atttatctag cggagaagtg gctatctcgc ttgatttagt cactggagag
     3181 tcttctggca aagatacttt ggcttttgac catagtcaaa tcatcttgaa agcattcaac
     3241 cgtgtagtca ataaaatgga acacgagccg caagttctac aagttttagg taaagatttc
     3301 acgattacgg aagctcgaaa agtatttgct aaattcttag ggatcgatta taaaacgatc
     3361 gatcattcca attttaagaa agctatgctc caatatttcg atgagatcgg tgaacgtcct
     3421 gtaggaatcg gccgtccttc aaaaatctat cagctcaagc cggggcacca tgaataaggt
     3481 tgtgagcaaa agattcagac ctaatcattc aagaaaaaac gcaaaaacaa ctctttgtga
     3541 gttacgtttt ttgtttcaga attttgatgt attcgtaaac tttctgaata aaaaattaac
     3601 aatagcagaa aaagctcgta cgaacttttt ctgctattgt tttattggtt tgtcataatt
     3661 agtgagcttt tcttcaaaag aaaaattcct agaaacaaaa gtcattaaga ctttttatta
     3721 acaaggctaa ccctaacgct cctatcagtt tcattcattt tttatccctt gataaccgaa
     3781 tgtctgccca ttttttctac tacttggctc tttcttacgg aattgctttc ttctgctccg
     3841 tcggtattga tcaaaagaaa ttttttatta taccattggg acttataacg caattttgtt
     3901 attaatttat gccagtcgtg aatagaaaaa tcagcatgtg cctcttcttt tggaaaagat
     3961 tctacaaata agtagtcata ttgtttgacc aatttcccag ataattgttc aagataatct
     4021 tctcgctggt tcttttgttt cagataaaga tccattactt ttctcttttg tttctgataa
     4081 ttgcttgcct gatctaacct gatctttcta tggtgagcgg agcggacttt cacattcaat
     4141 ttccgctgtg catgttgcag cttttcttct gtctggtgaa attttggtaa agttacttct
     4201 agaggtcgag atgtttggat caatgtcttc ggatgatagg cgatcccgat ccatttagct
     4261 aaagattctt tttttactgg cgaaacatca caaagtagtg cgacataaaa ttcttcatta
     4321 tatcgagcag aaatcgtagc ggagcgaatc gatccttcaa ctggccgatg ctgatttatc
     4381 ttgattagtt ctttttgttt tggtaacttt aagtagccat tttccagata gatcgtacgg
     4441 gtttggttgt tggtcgtata agattgccac atactttttt tcgttttaag ctttgggaag
     4501 ctggcacgtc ccttgaaata attgcgaaaa gcccgatcca aattaagctg tgcatttgct
     4561 aaagcaagac tgtctacttc ttttaagaat gggtattctt tttttaatgt agcgggtgtg
     4621 agcgaaaaat tggctactcc tgtttgttgc caacttttct gccgcttttt cagcaaatgg
     4681 ttataggtaa atcgagcaca tccaaaagta cgaatcaatt gctgtttttg ttcttctgtc
     4741 ggatacatac ggaatttaaa agcttttagc atctatgtcc ctcctagtta gcaaccagaa
     4801 gagattcctt ttgtcgtttt agtttttttg cgtaatatgg gacttcctgc tcagttgcat
     4861 agacaaaatg ttctggtgcg atttcacgaa gttgacgtac ttgtccgtca tctggttcag
     4921 gttgatattt gattcttctg cgttttctct catggtctgg atctggtaga ggaatagcag
     4981 ataacaaact ttctgtataa ggatgaacac cgtgattata gatttcatca cttgttccca
     5041 tctctaataa caagccgtta tgcattacac cgattcggtc actaatatgt ttaaccatcg
     5101 acaaatcatg tgcaataaat agataggtca attgatgttc tttttggaga tcttgcaaca
     5161 aattgaccac ttgggcttgg atcgacacat ctagtgcaga aattggttca tcacaaatga
     5221 taaaacgcgg ttttactgcc aatgctcgtg caataccaat acgctggcgt tgcccacctg
     5281 aaaattcatg aggataacga gtaccgtggc ttggattcaa tccaacagtt ctcaatagat
     5341 catcgacttt ttttgcacgc tcttcagatg atttagctag tccattaaca tcaattcctt
     5401 cggcaatgat atctctgact ttcatacgag gattcaacga agcgtatgga tcttgaaaga
     5461 tcatctgaac atcatgccgg aatttcgtca aacctttttt atctttgatc ttcgttatat
     5521 cttgaccgtc gaataatatc tcaccatcag taggttcatt caagcgaatg atcgtacggc
     5581 cagtagtgga ttttccgctt ccagattctc caactaaacc gaatgtttct ccttcataaa
     5641 tgtgaaagct gatgtcattt acagcatgta cttcattctt ttttcctaca ttgaaatact
     5701 gtttcaatcc tttaacttca agtagtttat ttggcatcag caattcctcc ttgaccgtaa
     5761 gtatgttttt gtttctcttt aaagatagcc cagcgacgtt ggatttcttc tggcggtgtc
     5821 actttgggtg cctgcggagc aagtagccaa gttgctgcct tgtgtgtttt tgatagttca
     5881 aaaaatggtg gttcttgttc tgcatcgatt ttcatcgcga attcatttct tggatagaat
     5941 gcgtctcctt ttggcgggtt caataaatcc ggtggagaac caggaatagc atacaatcga
     6001 tcgttcgcac tatctaatgt cggcattgaa ccaagcagtc cccaagtata gggatgttgc
     6061 gggttgtaga aaatttcttc cgatgttcca acttcaacga ttcttcctcc atacatgaca
     6121 gcgacacgat cagcgacatt cgctactaca ccaaggtcat gagtgataaa gataattgat
     6181 gtatcgattt ttgtctgaat ttctttcagc agctccaaaa tctgtgcttg aatagtcaca
     6241 tcaagcgctg ttgtaggttc atcagcaatc aggatttccg gataacatac cagtgcaatc
     6301 gcgatcacga tccgttgacg ctgacctcct gagaactgat gcggataact gttgatacgt
     6361 ttctcagctt cgggtattcc aactaattta agcaattcta atgcagcttt ttgtccctct
     6421 ttttttgaaa ctttattatg ctttctaagg gactcagcga cttgcttccc aatgggcatc
     6481 gtcggatcta aagaggtcat tggatcttgg aaaatcatcg ctatttcttt accacggatt
     6541 ttttgcatct ctctttctga ttttccaacg atatcgttac ctttaaagag gatttgacca
     6601 gcatctatat tggcgttgct tgacagcaag cgcatgatgc ttctggtcgt cactgatttt
     6661 ccacttccag attccccaac gatcgccaat gtttctcctt tattcaaatg aaaattgaca
     6721 ccgcgaatcg ctcgtacctt tccagcatac gtatcaaaag agatttctaa gtctttcact
     6781 tctaaaattt tcgtcatcgt cttcactctt tcattttagg atcaaaggca tcacgcaatc
     6841 catcagcaag taagttaaag ccaatcatga taaccgataa agttactgcc ggaatccata
     6901 atagataagg cagatacagg aatgtcttgt acccttcgct caacatcgtt cctaatgaag
     6961 ctgacggagg cgtaagacct aaaccgataa agcttaaaaa ggcttcgaag aaaatagcag
     7021 atggaatact aaacatcatt tgaataataa tgacactaga gatattcggc aaaatatgtt
     7081 tgaatgcaat tttcattctt gattcaccta atgtcaatcc agctaaaaca tattcttgat
     7141 cttttaattt cattgtttgt gcacgaacga tacgtgccat tggtatccag tttgtgatgg
     7201 ccatagcagc cacgattgaa aaaattcctg gttcaaaaac gaccaacatc aaaatcatga
     7261 caacgaggtt tggaattcct gataagattt ccaaaaaacg ttgcatcacg ttatcgacac
     7321 gtccgcctaa caaaccagaa accaacccgt aagctacacc aatcgttaca tctagtaatg
     7381 cagcaataaa tgcgatcagc aacgagattc tcattcccat aaataagcgg cttaatacat
     7441 cacgtcccaa accgtctgtt cctaaaaaga aatttacgtt tgaagggacg tttgcttgag
     7501 catatttatc aacaagttca ccaccaacca ttgcttttcc attcaatcca tcgatattga
     7561 ttcccggaat acgaggaggc aaattgatat aagccacgtt ttgggcagtt ggatcatgag
     7621 gagaaacaaa gattgtgatc acagagatca aaatgatgac caatagtaaa accattgaaa
     7681 taacggcagc tttgtttttc tttaaacggc gccatgaatc ttggataaaa ttcaaagaag
     7741 gtgcagtaat ctgttcacgt tcttcttcgg tatttttttg taatggaaga aattcatccg
     7801 cagggatatc tgcaactgtt tcataacgct ttggtatcat ttccatttat cctcggctcc
     7861 cttctgaaac acgaattctt ggatcaacta gaccgtacaa tagatccacg atcaaaataa
     7921 caaaaatcag cataaatgag taaaggatcg ttacagccat gatcgtagga taatcatttg
     7981 ttgtgattga cttaacgaat tgttccccga taccaggaat agcaaagata ttttctacca
     8041 ccagtgatcc ggtcatcaat gctacagcta acggtcctaa taaagtcatc aatggaatca
     8101 aactatttcg aagaccatgt ttaaaggcga tttcccaacg gctcaatcct tttgcttttg
     8161 ctaattccac ataatcacta tgcagtactt ccaccatttc cgtacggata aaccgagcag
     8221 agtcagcaag cggcgacatg gccaacgcaa ttgttggtaa gattgtgtac acaaagccat
     8281 cccattttgc gattggcaaa acttgtaatt tgattgcaaa gatatattgt aataaaacag
     8341 caaatacaaa gtttggaatg gaacgaccta atatcgctac gagtgtagaa gatgtatctg
     8401 cccatgtatt ttgcttcatg gcagagatcg ttcctaaaat cgtccctagg actgttccaa
     8461 agataattgc ctgtaaccct aactgcaagg atggtccgat tcttccagct aacaaatgtg
     8521 cgactggctg gtttttaaat tggaaggaaa ttccaaaatc tccttgaagc aaattagaaa
     8581 gataaatccc atattgtacg ataactggtt tatccaaacc gacttgttta tttagcatct
     8641 cgattgtttc aggactcaaa cgttcctgat tcgtatatgg cgtgcccggc agcaattgca
     8701 tcaagaaaaa ggtgattgtt gcaattaacc aaagcgtgat gatcatgaaa aagacacgtt
     8761 ttaatacata tttgatataa ctattcacta atgtttcccc ctagcttttg acttctgaca
     8821 gatgtttcgc tacattatta gataatgaac caaaaataaa ggagcaatca aatgaaaaaa
     8881 aaatctatcc cctacgcagt agcttttcta ctgattctgg ttattttaat aaaaaatctc
     8941 atcaaccatt cctttacact cattcaatta tctaatgatc tattcttatg gtcattgccg
     9001 tttctgatta tcggcggttt tttatgggtc ttttcttcgg gtttcttcga tcattttcag
     9061 cgatcggtcc atcttgcacg aacaaggaac cgaaagaaaa aaccagaatt ttcctcgctc
     9121 tcgtctgcca gctacggtat gtacagcttt tggctgatca ttgccggaat actaattgct
     9181 ctttctgcta ttttcatgct tttttctttg ttaggataag tgaatatgag aacgaggttg
     9241 cgacatttgc cgcagcctcg ttttttctca tgctcacggt attaacgatt attcttcaac
     9301 gtatgtccat ttgtaatccc atgaagcgcc tgccgcatgg tgaacgatac ctttgatttt
     9361 ttcattcact aggtgacctt ctgctttttg gtacaccgga acgacaccca tatcatcaga
     9421 gatgatcttg attgcttttt ggaaatcatc ccaacgggct tgttcatcgc cagcgtctgt
     9481 tgatgaagct gctttaactg cttcgtcata ttctgcattg ctccagcgac cacggttgta
     9541 tgaattacct gtgacaaaca aatcagtaaa gctgcttggg tctgcatagt cagcagccca
     9601 tccgcctagt actgcgtcaa aatcacccga agttgagcgg tcaagacgta cagagaatgg
     9661 aacaggtgtt ggtttgactg ttacgccatc caagttttct tggattgagt tttgaacata
     9721 ttcgattact tttttcgatg aatcatcatc agaagccatc aaatcaaatt ctaaagaatc
     9781 aacgcccaat tcttttttcg ctttttccca atattctttt gctttatctt tgtcataaga
     9841 aactaatttt ccagcttctt ttgcaaagtc ttcgttcgtt gttggacttt ttaccatatc
     9901 tgatgggatc aatcctgtag agacaactga tccgtcacct agtacttgtt ttactaaagc
     9961 ttcacgatcg atagaataag aaatagcttt tcttaggttc acattgttga atggtgaatt
    10021 cttttcacgt tgattgaatt cgatatagct tgtccgcgct tctttaacag aagtataagc
    10081 tggatcgttt gcgttttgct gtgctaactc accagacaag atcacgtcat ctgcttggcc
    10141 atctttgaat aagttcaatg cagtagaaga ttcttttact acactgactt tgatttcaga
    10201 aagttttact gtatctttgt cccagtaatg atcatttttc ttataagtcc attctgtatc
    10261 tgttcctggt ccatcaaaat cttctaatgt gaatggtcca ttataaacag ctttatcact
    10321 tgcagatgca taggcatccc cattttcttc cactacggat tgtttttgtg ggaaaaatga
    10381 tgggaaagca agcagatatt ggaaatatgg tgtttgtttc gtcaatttga tttctaattc
    10441 atagtcacca actgctttga tccctagttc tgatttatct ttctttccag ctgtaatgtc
    10501 ttctgcattt tcaactggcg catacaggta agcatattca gaagctgtag ctgggtctac
    10561 tgttcgttgc cagccataaa cgtaatccgc tgctgtaaca ggatcaccat ttgaccattt
    10621 tgcgtcttct cgtaatttga ttttgtaagt taatccgtct tcactgactt ctgccaactc
    10681 gctagctcct gccggctgtg gtttgctgtc ttcatcaaga cggtaaattc cttcataaac
    10741 gttattcaat gctgtaaaac tgattgtatc tgttgcaact gataagtcag ctgtaggcat
    10801 ttcttgaggc acgactaagt taaatacgcc tcctccatct gctgaaccgc tttttgctga
    10861 actactgctt gtggctgaac tgccgccata acatccagca agtacaaggc cgcataccgc
    10921 aaccaaacca aatgtaaatt tctttttcat tttacgtacc cctttattgg aattttcaga
    10981 ataattaata ttttctgaag ttgtttaaga gtttaatgaa taattattaa aaaatcaaca
    11041 cttttttttg tctttttcat aaaataatga ttttttcgtg aggtttcaag gaatttttca
    11101 gaaaaaatag ctttgtacaa ggttttccat ggtcttccaa aagccattaa ataattttcg
    11161 ttatattgtc tattattgcc atacaaagat aaaaaagaat aaacaaaaag aaactgtgac
    11221 aaaagtcttg tcacagtccc ttatctga
//
LOCUS       DAZKLD010000098        11214 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.098, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000098 DAZKLD010000000
VERSION     DAZKLD010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11214)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11214)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11214
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.098"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            6..305
                     /locus_tag="ACG8KR_002063"
     CDS             6..305
                     /locus_tag="ACG8KR_002063"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFQ0201420.1"
                     /translation="MKNRLIVACGSGVATSQTIASKIQSMLEDDGIAFPVEAVDYKSI
                     QNELLTAGIYVYVAQPDEEVLEQAKDLGIEVFPGIPFLTGMGVEPIYDSIKELVQ"
     gene            378..1739
                     /locus_tag="ACG8KR_002064"
     CDS             378..1739
                     /locus_tag="ACG8KR_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HFQ0201421.1"
                     /translation="MEVFQTIINYVLNLGSAIFVPLIILILGLLAGMKLKKAFMSALT
                     LGIAFSGMSMVIGYMSNAVSPASEALAKNTGISLPALDLGWTGAASITWSWSYAFIFF
                     AVTIGVNFVLLLFNWTKTLNVDMWNVWGKALTAYLVYYVSGSLAAGFLTAMVQVILEL
                     KLGDMFQKHIQDLTGIPLVTVTHFMTSAAVLLLPFNMIMDKIPALNKRADTNALKKKI
                     GIFSENSVMGFIIGLGLGFAAAYGVSGSLNLAIQTATALSLFPMISKMFMQSLSPLAD
                     AMSEFMKKRFKDREVYIGLDWPILAGRSEIWVTAILLVPVFIGYAIILPGNQVLPLAG
                     IINYSIAVGGLLLTGGNLARMLILGIITMPIYLYGATYLAPILSDLAEKSGAVEGIKK
                     GQLITWSSIEGPEFRVLFAEAFNRNILAIVGCVIFLGLFVLLYKYMIKAKVPSERYAE
                     LQK"
     gene            1761..2021
                     /locus_tag="ACG8KR_002065"
     CDS             1761..2021
                     /locus_tag="ACG8KR_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0201422.1"
                     /translation="MKREETDKIKWTVALCGTLLLFLYGLFTQNIIINLLVIFFALVI
                     YKYGNHVLFREYDEKRKRKIEESMKIKEATKEILREKSFIKR"
     gene            2035..2787
                     /locus_tag="ACG8KR_002066"
     CDS             2035..2787
                     /locus_tag="ACG8KR_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HFQ0201423.1"
                     /translation="MSDGRMLFERERQDMARIVRVIFERKNTNVAGGNFSFKTTDDQG
                     KDYIIMTPTMMSEAYLGEISPAQVLVVEPRTRRIVDGVGHLTREINMHEAMYETNPAI
                     KAVLHAHAPNAMFWATTGLPMPNLTEATAKVKEIPVMDFEPNCSEELAELTSDYVKKQ
                     HLELPHMLLLNSHGVLINTGGESGIEAIHKALQILDTVEWNAEIAYKQTIFQALGITD
                     GFYSKGEKIGGLEELKHKTAIYNKSVIAAGGD"
     gene            complement(2881..3486)
                     /locus_tag="ACG8KR_002067"
     CDS             complement(2881..3486)
                     /locus_tag="ACG8KR_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFQ0201424.1"
                     /translation="MTYIFEKGTSGKHLILLHGTGGDEHSLLDIAHFLAPNSTLLSFR
                     GTVQEDGMNRFFKRNGLNQFDLDSLEKETDKLHEKIKEISEKELIPLSDWLLVGYSNG
                     ANIAAHLLLERETELNKGLFFHPMSLGVHQSRSTLEDKTVWISYGDNDPIVSPASFKE
                     LADQFETRQANVTIQLTVTGHQLTMDEIKQAREWTDHLLTK"
     gene            complement(3539..4513)
                     /locus_tag="ACG8KR_002068"
     CDS             complement(3539..4513)
                     /locus_tag="ACG8KR_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HFQ0201425.1"
                     /translation="MDTQIRGIHHVTAMTSSATKIYRFFTDILGMRLIKKTVNQDDIE
                     TYHLYFTDDTGAPGTDMTFFDFPNQPKGKKGTDTISRASFRVPSDAALDFWLERFEKY
                     DVTHSAINERFGKKYIEFEDFDNQYYQLISDENNQGVAAGIPWKNSNVPTEFAITGLG
                     PVFVRVSQLEHIRLILEKILGFKETDHSGDFYLFEIGEGGNGASIIIEHRTDLPKAIE
                     GFGNIHHLALRVADKEALEYWIQVINKIELPNSGFVDRFYFQSEYFLAAHHVLFELAT
                     DGPGFLEDEPAESAGEKLSLPPFLEERREAIEAYVRPFDTSDANKKRE"
     gene            complement(4891..5610)
                     /locus_tag="ACG8KR_002069"
     CDS             complement(4891..5610)
                     /locus_tag="ACG8KR_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFQ0201426.1"
                     /translation="MPKYEEIANILRERIRSKEYPLDSFLPNQVDLVKEFGASRMTIK
                     KAINILAMEGLVYSQRGSGTKILNHPFMQKDTSPANEYEGLSLQMKRRHRSLKSQIIH
                     FDVEFPNQYIQERLMIDAEQPVYNIHRLRILDGEPYVLEHTFMPVHLVPGLKKEHLLA
                     SIYDHLHQELNVHFAGAYRAISADKSDEYDQQYLNCAENDPVLEVQQVVYLKDGQPIE
                     YSSSRNRFDVRNYSVLDVRST"
     gene            complement(5737..7008)
                     /gene="celB"
                     /locus_tag="ACG8KR_002070"
     CDS             complement(5737..7008)
                     /gene="celB"
                     /locus_tag="ACG8KR_002070"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0019191 - cellobiose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HFQ0201427.1"
                     /translation="MNGFMDKLSEKIMPLANLLGQNRYLTVLRDAFMLSFPLTMFGSI
                     VVVINNLPFFSDATKGTLSNLFGNGQNATMSIMSVFVTFGIGYYLSKSYDVEGIFGGA
                     VSFASFLILTPFAMKTAGGEDVTGVLSLDRLGAKGMFIGMIAAFLAGEIYCRITKRGW
                     QIKMPDGVPPAVTKSFAALIPAVVTLTIFLVINAVMTGIFNANLHDVVYEVIQKPLTG
                     LGSSLPATLIALFFVQFLWFFGLHGQIIVNSVMDPIWNTLMLDNLEAYQHGKELPHII
                     TKPFMETFTVGIGGSGMTLAVVLLMAFVLKKKQYRDVGRLALAPGIFNVNEPAIFGLP
                     IVLNATILIPWVVAPLIVTTFNYLVMAAGLVPAPTGVSVPWTVPIIASGVLATNSWLG
                     GMLQVIDFVIVAFIWYPFLKVLDKQPDLDIA"
     gene            7161..8558
                     /locus_tag="ACG8KR_002071"
     CDS             7161..8558
                     /locus_tag="ACG8KR_002071"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFQ0201428.1"
                     /translation="MKTYTFPENFWWGSAASGPQTEGRVSDDGKGENIWDHWYELEPE
                     KFFDQVGPEKTSQVYTKYKEDVQLMKETGHNTFRTSIQWSHLIPEGTGEINQKAVSFY
                     RDFFTELLDNGIEPFVNLYHFDMPLPLQEKGGWLSRETVDAYVSYAAACFELFGDLVK
                     KWFTHNEPIVPVEGGYLYQFHYPNHINMKEAVQVGYHENLASAKVIKAYHEGGHDGEI
                     GIILNLTPSYPRDETNPEDVKAAKIADAFFNRSFLDPAVKGEFPQELIEIVKELAIMP
                     EIHEGDLELIRENTIDLLGINYYQPRRIKAKETPINHEHSPMPDDYFDNYDMPNKKMN
                     PYRGWEIYEKGIYDILTNTRENYGNIKCYISENGMGVEGEERFVNSEGIIEDDYRIEF
                     VTDHLKYVHQAIQEGTNCVGYHMWTCMDNWSWTNAYKNRYGFIAVDLAQDGKRTIKKS
                     GRWFKTVSEKNGFDA"
     gene            8639..9079
                     /locus_tag="ACG8KR_002072"
     CDS             8639..9079
                     /locus_tag="ACG8KR_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFQ0201429.1"
                     /translation="MVDTYRNILVAIDGSEKAEKAFLEAITIAKRNQATLHILYVNEV
                     TGNYFGDFAFVTTSLQEELDEVAENQMKEHRNLAIEKGLTDIETYVLYGYPKTLIANF
                     HESKEPIDLIVMGATGLNAVERALVGSTTSYVVNHASCNVMVVK"
     gene            9227..9553
                     /locus_tag="ACG8KR_002073"
     CDS             9227..9553
                     /locus_tag="ACG8KR_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFQ0201430.1"
                     /translation="MISSDGIRGYNDAIILSILNKNDSYGYEISKTIRERTNDSYVIK
                     ETTLYSVFARLEKNQLIVSYPGEVTHGKKRTYYKITEIGRNLLEEKKQEWLTTKDVVD
                     QFLEGN"
     gene            9554..10303
                     /locus_tag="ACG8KR_002074"
     CDS             9554..10303
                     /locus_tag="ACG8KR_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease prefix domain 1-containing protein"
                     /protein_id="HFQ0201431.1"
                     /translation="MDTIRNYLDSLFIGVPQSTEIDKLKTDLLANMEDHYHELMGEGK
                     NEQEAIGTVISTFGSIDELLEELDVEKKHQADETETNTASIYLSEAENYWKEYRAASL
                     QVASGVLFISLSFASFLFFCSAGYVFMGISCLIFGIALAVGFFIASGMKITRLNHFLH
                     HRKIPEKVLAEAKEKEEEYQRSFGFSLIAGIGLCIFSLFPLLASLMWYMDGSIGASIF
                     FVTVGTGVFLIIYGSLVRHSYRQFTQSAYYW"
     gene            complement(10383..10838)
                     /locus_tag="ACG8KR_002075"
     CDS             complement(10383..10838)
                     /locus_tag="ACG8KR_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFQ0201432.1"
                     /translation="MLTIYSSRTHSYHLMANWLKDHQMPYRERIFTPKQPLTRKEIFY
                     LLSLTENGFDDLLAKRSIDYKNLKDQIDQCTTSELVDLIVSHFTLLQRPIITDGKKLL
                     VGFNEDSMRLFIPVQWRRSQLKSVYNDFTWVSNLENSTDLDDEFLNYYI"
BASE COUNT         3558 a         1960 c         2301 g         3395 t
ORIGIN      
        1 aaaaaatgaa aaatcggtta attgtcgcat gtggaagtgg agtagcaacg agtcagacaa
       61 tcgctagtaa gatccaaagt atgttagaag atgacggcat cgcatttcct gtagaagcag
      121 ttgattacaa atcgattcaa aatgagcttc taactgctgg aatctatgtg tatgtcgcac
      181 aacctgatga ggaagtactt gaacaagcaa aagatttggg tattgaagta tttccaggaa
      241 ttccattttt aactggaatg ggtgtagaac ctatttatga cagtatcaaa gagttagtac
      301 agtaaaggag gattgttgga taaaaaagtt ggttcatagt tttatttact atatttagaa
      361 agaaagaagg tttatttatg gaagttttcc aaaccattat caattatgtt ttgaacttag
      421 gatcagccat ttttgttccc ctgattattt tgatacttgg attgttggca gggatgaaat
      481 taaaaaaagc attcatgtct gctttgactt tagggattgc ttttagcgga atgtctatgg
      541 tcattggcta tatgtctaac gcagtaagtc cagcatcaga agcattggct aaaaatacgg
      601 gaatcagttt gcctgcactt gatcttgggt ggacaggtgc agcaagcatc acatggtcgt
      661 ggagttatgc ttttatattt tttgcagtaa cgattggggt caattttgta ctgttacttt
      721 ttaattggac gaagacgtta aatgttgata tgtggaatgt ttgggggaaa gctttaactg
      781 cttatttagt ttattatgtt tccggtagtt tggctgcagg ctttcttact gcaatggtcc
      841 aagttatatt ggaattaaag cttggtgata tgtttcaaaa acatatacaa gatctcacag
      901 ggattccatt agtaacagtg actcatttta tgacatctgc cgctgtcttg ttgctgcctt
      961 tcaatatgat catggataaa attcctgctt taaataaacg agcggatacg aatgcattga
     1021 agaagaaaat tgggatcttc tctgaaaact ctgtaatggg attcattatt ggattggggc
     1081 ttggctttgc tgctgcgtat ggcgtttcag gttctcttaa tctagcgatc caaacagcta
     1141 cagcattaag tttgtttcct atgatctcta aaatgttcat gcaatctttg tctcctctag
     1201 cagacgctat gtcagaattt atgaaaaagc ggtttaagga tcgtgaagta tatatcggat
     1261 tggactggcc gatattagct ggtcgttcag aaatttgggt tactgctatt ttacttgttc
     1321 ctgtgtttat tggttatgcc attattttgc caggaaacca agtattgcca ttagcaggga
     1381 ttatcaacta ttcgattgcg gtcggcggat tacttttaac aggaggaaac ttggctcgta
     1441 tgctgatctt gggaatcatc actatgccta tttatttgta cggggccact taccttgcgc
     1501 caatactcag tgatttagca gaaaaaagcg gagcagtcga gggaatcaag aaaggacagt
     1561 tgatcacatg gtcatccata gaagggccgg agttcagggt tttatttgcg gaggcattta
     1621 ataggaatat tctcgctatt gtaggatgcg tgatttttct aggactgttt gttctacttt
     1681 ataaatatat gataaaagcg aaagtaccga gtgaacgtta tgctgaacta caaaaatagc
     1741 attgaaatgg tgggaaagta atgaaaagag aagagacaga taaaataaaa tggacagtcg
     1801 ctctttgtgg tacacttctg ctatttctat atggactctt tactcagaat atcatcatca
     1861 atctattggt catttttttc gcactagtga tctataaata tggaaatcac gttttattca
     1921 gagagtacga tgaaaaaaga aaacggaaaa tagaagaatc aatgaaaata aaagaagcta
     1981 ccaaagaaat attgagagaa aaaagtttta taaaaagata atggaggggt cattatgtca
     2041 gatggacgta tgttatttga gagagaacga caggacatgg cacgtatcgt aagagtgatt
     2101 ttcgaaagga aaaacacaaa tgtcgcagga gggaattttt cctttaaaac cactgatgat
     2161 caagggaaag actacatcat catgacgccg accatgatgt cagaagccta tctaggagag
     2221 atttctcctg cacaggttct agtagtagaa ccaagaacgc gtagaatcgt tgacggagtc
     2281 ggtcatttaa ctcgtgaaat caatatgcat gaagcgatgt atgagactaa tccagctatc
     2341 aaagctgtcc tacatgctca tgcgcctaat gcaatgttct gggcgactac tgggttgcct
     2401 atgcctaatt tgacagaagc cacagctaaa gtaaaagaga ttcctgtcat ggactttgag
     2461 cctaattgct ctgaagaact tgctgaattg acaagcgact atgtaaaaaa acagcatctc
     2521 gaattaccgc atatgttgct gctcaactct catggcgtat tgatcaacac aggtggagaa
     2581 tcaggaatcg aagctatcca taaagcactg caaatccttg atacagttga atggaatgca
     2641 gaaattgcct acaagcagac gatttttcaa gcattaggga ttactgacgg tttttattca
     2701 aaaggtgaga aaatcggggg attggaagag ttgaaacata aaacggctat ttataataaa
     2761 tctgttattg cagctggtgg agactaacct atctgacaca aaaaactacg actttcacaa
     2821 aatctgatcc agatcgatat ctgcttcagg ttgtattatg taagtcgtag ttcttttctt
     2881 ttatttagtt aagaggtggt ctgtccattc cctcgcttgc ttgatttcgt ccatcgtcaa
     2941 ctgatgacct gttacagtta gctgaatagt cacatttgct tggcgtgttt cgaattgatc
     3001 ggccaattct ttaaaggaag caggggaaac gattggatcg ttatcaccat aagaaatcca
     3061 gacggtttta tcctctaatg tggaacggct ttgatgaaca cctagagaca tcggatggaa
     3121 aaacaaccct ttatttagct cggtctcacg ctccaataat aaatgagcag cgatgtttgc
     3181 gccatttgaa tagccgacaa gcagccagtc acttaacggt atcagttctt tttcgctgat
     3241 ttctttgatt ttttcatgga gtttatctgt ttctttttcc aaactgtcca ggtcgaattg
     3301 attcaatcca ttacgcttga aaaaacgatt catcccatct tcttggaccg tgccacgaaa
     3361 agaaagcaat gtactgtttg gtgcaagaaa gtgagcgata tccagtaaag aatgttcatc
     3421 gccacctgtg ccatgaagta agatcaaatg cttgccactt gttccttttt caaaaatgta
     3481 ggtcattttg ttccctcttt tctgtttcta tagatgcaaa tgaagtagca tgaagagatt
     3541 attcacgttt tttatttgca tcacttgtat cgaaaggacg gacataagct tcaatggctt
     3601 cacgacgttc ttctaagaat ggaggaagag atagtttttc tcctgctgat tctgctggtt
     3661 catcttcaag gaatccagga ccatcagttg ctaattcaaa cagtacgtga tgagcggcta
     3721 ggaagtactc agattggaaa tagaaacgat cgacgaaacc agaatttggc aattcaatct
     3781 tattgattac ttgaatccag tattccaatg cttctttgtc tgctacacgc aatgctaagt
     3841 gatggatatt tccaaatcct tcgattgctt ttggcaagtc tgtccgatgt tcaataatga
     3901 tgcttgcacc atttccccct tcaccgattt caaataagta gaaatctcct gaatggtctg
     3961 tttctttgaa tcctaagatt ttttcaagaa tcaagcggat atgctctaat tgactgacac
     4021 ggacaaagac aggtccaagt cctgtgatcg caaactctgt cggtacattg ctgtttttcc
     4081 aaggaatgcc agctgctaca ccttgattgt tttcgtcaga gatcaattga taatactgat
     4141 tatcgaaatc ttcaaattcg atatattttt tgccgaaacg ttcattgatt gctgaatgag
     4201 taacgtcata tttttcaaaa cgttccaacc aaaagtctaa tgctgcgtca cttggcacac
     4261 ggaaagaagc acgagagatc gtatctgttc ctttttttcc ttttggctga tttggaaaat
     4321 caaagaaagt catatctgtt cctggcgcac cagtatcgtc tgtaaagtaa agatggtaag
     4381 tttcgatgtc atcttggtta actgtttttt tgattaagcg cattcctaaa atatctgtaa
     4441 aaaagcggta gatttttgta gcgctagatg tcattgctgt gacgtggtga attcctctga
     4501 tttgtgtatc catgataaat ttcctcctgt taacttacaa aaagtaagta gttgatttag
     4561 acatagtata gagggtgcat ctctattttg caattaattt gtcttctaaa gtatcaggaa
     4621 ggaatcaaga atggaaaaga ggagggacgg atcaataaaa taaaaagact tacgactagc
     4681 tgtatcgatt tcaaaaaaac tagctttaaa aagctaactt tagaatcatt acgggtggtc
     4741 ataagtcttt ttatgctgag ttattttttc atttttgagc taatgcttag gatagaatag
     4801 aatgttgatt tcacgatctc ttcttgttac ttgaaactgc ttaccactgt cacgatctct
     4861 tggtaaacgg ctttttcaca acctctttgg ttaagttgaa cgcacatcca gcacagaata
     4921 gttccgaaca tcaaatcggt ttcggctgga agaatattca atcggctgtc catctttgag
     4981 atagacgact tgctgaactt ccagtacagg atcgttttct gcacagttca aatattgctg
     5041 atcatattca tcacttttat ctgcagaaat agcacgataa gcccctgcaa aatggacatt
     5101 caattcttga tgaagatgat catagataga ggctaacaaa tgttcttttt ttaaacccgg
     5161 cacaagatgg actggcataa aggtatgctc taaaacatat ggctcgccat ctaagatcct
     5221 taaccggtga atattgtaga caggttgctc ggcatcgatc atcaatcgtt cttggatata
     5281 ttgattaggg aattcaacat caaaatgaat gatttgactc ttcaaagaac gatgtctgcg
     5341 tttcatttgg agactcaaac cttcatactc gttagcgggc gatgtgtctt tttgcataaa
     5401 tggatgattc aagattttcg ttcctgatcc tcgctgcgaa tagacaagtc cttccattgc
     5461 taagatattg atggcttttt tgattgtcat ccggctcgca ccaaattctt ttaccaagtc
     5521 gacttgatta ggcaaaaaac tatcgagagg atactctttt gaacggatac gttctcttaa
     5581 aatatttgcg atttcttcgt attttggcac gttattcacc tcttctagag tataacgaaa
     5641 gattatgctt ccgacaactc gaagtagcat ctggaacatt gtttattcga aaaaagaggt
     5701 catgacaagt ttttgtcaca accccttaat gatctattaa gcaatatcta aatctggttg
     5761 tttatctagt actttcagga atgggtacca gataaaggca acgatgacaa agtcgatgac
     5821 ttgcagcata ccaccaagcc atgaatttgt tgctaagaca ccgcttgcaa tgattggcac
     5881 agtccatgga acagagacgc cagttggtgc agggacaagt ccagcagcca ttaccaaata
     5941 attgaacgtc gttacgatta gaggtgccac aacccaagga ataaggattg ttgcattcaa
     6001 tacgatcggt agaccgaaga tcgctggttc atttacattg aaaataccag gtgctaaagc
     6061 taatcgtcca acatcacgat actgtttctt tttaagaaca aaggccatca ataaaacgac
     6121 agctaaagtc attccagagc caccaatacc aacagtgaag gtttccatga atggttttgt
     6181 gatgatgtgt ggtaattctt ttccatgttg ataagcttct aagttatcaa gcatcagtgt
     6241 attccaaatc gggtccatta cagagttgac gatgatttgt ccgtgaagtc cgaagaacca
     6301 taagaattgg acaaagaaca aagcaatcaa tgttgcaggt aaactacttc ccaaaccagt
     6361 taatggtttt tggatcactt cataaacgac gtcatgtaag ttagcattga aaataccagt
     6421 catcacagca ttgattacta agaaaatagt tagtgtcact acagcaggga tcaaagcggc
     6481 aaatgatttt gttactgcag gcggaactcc atcaggcatt ttgatctgcc agccgcgttt
     6541 cgtgattctg cagtagattt ctccagccaa gaaagcagcg atcattccaa tgaacattcc
     6601 ttttgcgcca agacgatcaa gtgaaagcac accagtcaca tcttcaccgc cagcggtttt
     6661 cattgcaaaa ggcgtaagaa tcaagaagct ggcaaaagac acggcaccac cgaaaatacc
     6721 ttcaacgtca taagattttg ataaataata gccgatacca aaagtaacaa aaaccgacat
     6781 gatgctcatt gttgcatttt gcccattacc gaataaattc gataaagttc cctttgttgc
     6841 gtcactgaaa aaaggcaagt tattgataac gacaacgatc gaaccgaaca tcgttaaagg
     6901 gaaggaaagc atgaaagcat cccgcagcac tgtaaggtaa cgattttgcc ctaataaatt
     6961 agctaagggc atgattttct cagaaagctt gtccataaaa ccgttcattt ttattcacct
     7021 caaatttatt tgcaatcgat tacatacaaa gtataacgcc aaaaaaatta aatgtctagt
     7081 cattttcata aaaagtattg atttattttt tagggttcgt tatgattacg gtatcaagaa
     7141 aaaagagagg aagtaaaaaa atgaaaacct acacattccc agaaaatttt tggtggggtt
     7201 ctgctgctag cggaccgcaa acagaaggaa gagtaagtga tgatggtaaa ggagaaaata
     7261 tttgggatca ttggtatgaa ttagagcctg aaaaattctt cgatcaagta ggacctgaaa
     7321 aaacttcgca agtttacaca aaatacaaag aagatgttca gttaatgaaa gaaactggcc
     7381 ataacacatt tcgtacatct atccaatgga gtcatttgat tccagaaggg acaggtgaaa
     7441 tcaatcaaaa agctgtttct ttttatcgag atttttttac tgaattgctg gataatggta
     7501 ttgaaccttt tgtgaacctt taccatttcg atatgccact accactgcaa gaaaaaggcg
     7561 gatggttatc aagagaaacg gtcgacgctt atgtttctta tgcagcagcg tgctttgaac
     7621 tgtttggaga tctggtcaaa aaatggttta ctcataatga accaatcgta cctgtagaag
     7681 gaggctactt gtatcaattc cactatccaa atcatatcaa tatgaaagaa gcggtccaag
     7741 taggctatca tgagaatcta gcaagcgcaa aggtaatcaa agcctaccat gaaggcggac
     7801 atgatggtga gatcggaatc atcttgaatc tgacaccaag ctatccgaga gatgaaacga
     7861 acccagaaga tgtaaaagcg gcgaagatcg ctgacgcatt cttcaaccga tcattccttg
     7921 accctgcagt gaaaggagag tttccacaag agttgatcga aattgtcaaa gaattggcta
     7981 taatgccaga gatccatgaa ggagacctag aactcatccg tgaaaacacg attgacctgc
     8041 taggaatcaa ttactatcag cctcgtcgaa tcaaagcaaa agaaactcca atcaatcacg
     8101 aacatagccc catgccagat gattattttg ataattacga catgcccaat aaaaaaatga
     8161 atccatatcg tggatgggaa atttacgaaa aaggaatcta tgatatttta acgaatacaa
     8221 gagaaaacta tggtaatatc aaatgctata tttctgaaaa tgggatgggg gtcgaaggag
     8281 aagaacgatt tgttaacagc gaaggaatta ttgaagatga ttaccggatc gagtttgtta
     8341 cggatcattt gaaatacgtc catcaagcaa tccaagaagg aaccaattgt gtgggttacc
     8401 acatgtggac atgtatggac aactggtctt ggacaaatgc ttataagaat cgctatggct
     8461 ttatcgctgt tgatttggca caagacggta aacggacgat caaaaaatca ggtcgctggt
     8521 ttaaaacggt ctctgaaaaa aacggatttg atgcctgata ttttttgata aaacgttcac
     8581 aaaatgtttg ttctaacact gtaatcgtgc tacactaaca gtgaaggagg cgtttacaat
     8641 ggtagatact tatcgtaata ttttagtagc aattgatggt tctgaaaagg cagaaaaagc
     8701 atttcttgag gcaatcacga ttgccaaacg aaatcaggca acgttgcaca tcctgtatgt
     8761 caatgaagta acaggcaatt actttggtga ttttgcattt gttacaacaa gccttcagga
     8821 agaattggat gaggttgcgg aaaaccagat gaaagaacat cgaaacttgg caattgaaaa
     8881 aggcctgaca gatatcgaga catatgttct ttacggttat ccaaagacac tgattgctaa
     8941 tttccatgaa tcgaaagaac caatcgatct gattgtcatg ggggcgacag gtctgaatgc
     9001 ggtagaacgt gcactagtag gttccactac ttcctatgta gtcaaccacg catcatgtaa
     9061 cgtcatggtt gtaaaataaa taaattaagg gaacagaaag caaaaaagca taacgcttct
     9121 tttgctttct gttcctttta tttttttgta aaaaattact ttacctgatg aagtaatcat
     9181 gctattctaa ttacaacgac agatggagta atatggagga aagaaaatga tttcaagtga
     9241 tggaatacgc ggatacaatg atgcgattat tttatcgatc cttaacaaaa acgattctta
     9301 tggatatgaa atttctaaaa cgattcgtga gaggacaaat gattcctatg tgatcaaaga
     9361 gacaacactt tactccgttt ttgcccgttt agaaaagaat caactgattg tttcgtatcc
     9421 tggagaagtg actcatggaa aaaagcggac atattataaa atcactgaaa tagggcgtaa
     9481 tcttttagaa gaaaaaaagc aagagtggct gacgacgaaa gacgtagtcg atcaattttt
     9541 ggagggaaat taaatggata caatccgtaa ttatttagac agtcttttta tcggagtacc
     9601 tcaaagtacc gagatagata agctgaaaac tgatttgttg gcaaatatgg aagaccatta
     9661 tcatgaattg atgggagaag gaaaaaatga acaggaggcc atcggtacag taatttcgac
     9721 atttggttca atcgatgaat tattagaaga actagatgtc gaaaaaaaac atcaagctga
     9781 cgagacggaa acaaacacag cttctattta tttaagcgaa gcagaaaact actggaaaga
     9841 atacagagcg gcaagtctcc aagtggcatc aggtgttcta ttcattagct tatcttttgc
     9901 aagttttctt ttcttttgtt cagcaggata tgtgttcatg ggtatttctt gtctgatttt
     9961 cggtatagca ttagctgtcg gtttttttat tgcttctggt atgaaaatca cacgtttaaa
    10021 tcactttttg caccatcgga aaatacctga aaaagtatta gcagaagcaa aagaaaaaga
    10081 agaagagtat cagcgaagtt ttggcttctc actgattgca ggaattggat tatgtatttt
    10141 ttcacttttc ccacttttgg cttctttaat gtggtatatg gatggtagta tcggtgcctc
    10201 tatatttttt gttacggtag gaacgggtgt cttcttgatc atttatggca gtctagtccg
    10261 tcattcttat cgtcaattca ctcaaagcgc ttattactgg tgaatgtatt cagatttgta
    10321 aaaatattct atttatccgg cgtagaaaaa aagactgtga cacgcttttg tcacagtctt
    10381 ttttatatat aataatttaa aaattcatca tctaaatccg tagaattttc tagattgctt
    10441 acccatgtaa aatcgttata gacagatttc agttgacttc tgcgccattg tacagggata
    10501 aaaagacgca tgctatcttc attgaatccg actaataatt tctttccgtc agtaatgatg
    10561 ggacgttgta acaaagtaaa atgcgagacg atcagatcga caagctctga ggtcgtacat
    10621 tggtcgattt ggtctttcaa atttttataa tcaatcgagc gtttagctaa tagatcgtca
    10681 aatccgtttt ctgttaatga caataaataa aatatctctt tccttgttaa aggctgttta
    10741 ggtgtgaaaa ttcgttctct gtaaggcatt tgatggtctt tcagccagtt tgccatcaga
    10801 tggtaactat gtgtgcgaga agaatagatc gtaagcataa aaaaactcct taaatagtcc
    10861 attaatttgt tagatctgtt gagataaata ataggtaagg agctgtgcat aagcattgat
    10921 caaaacaacc caaacatcat tagaaataac tttctaaagt cttaaacttg cgacgagaaa
    10981 caactagact aatagaaaaa atgtcttagc catttggctt cctgtttgtt tttttgctta
    11041 tgctgcagct cctcgggaaa gggttctaac aatttgaaga aggttgatga ttgatctagt
    11101 ttaaattgtg tgaaatttga aatctggtgt ttttactaga tgaatcacct aacaactatg
    11161 attataactt atagaacttc tgttttgcat tcggttttgt tatagttgtt ataa
//
LOCUS       DAZKLD010000099        11114 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.099, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000099 DAZKLD010000000
VERSION     DAZKLD010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11114)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11114)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11114
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.099"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            81..950
                     /locus_tag="ACG8KR_002076"
     CDS             81..950
                     /locus_tag="ACG8KR_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HFQ0201433.1"
                     /translation="MKKKKFSRSLLGILVLVAVGLSGKGYFQNNSTQEHDFQIKSSVQ
                     VGNKADSESKEFLGIRYDPNKYPNNIVNINDGKYTLTKEDHEALSEKGSQTFWVNYAD
                     LDQLGRAGDVEALITYDAVNSHSSKNVERPSFKSSVHIAGEYKDGVFDQERDTWVGKD
                     SNNEITQLEGYKGYLYNKSHSLAWSLGGDMEVHNLTLGTRAQNVGKNDQNGGMAYSET
                     IVRDAIYKNKNEKVLYKVDPIYKGNELVPRGSHVEAFSVNDNGKTVNLNVYVFNNQKG
                     ININYQNGTWVRD"
     gene            970..1317
                     /locus_tag="ACG8KR_002077"
     CDS             970..1317
                     /locus_tag="ACG8KR_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0201434.1"
                     /translation="METISINGLDKSVANRIKKGNGYYLLKKGKYSVARELETMSIVY
                     VAKNHFDVEQAIFDPDSEIGKCVELSEKTVSTKGAMIIEREMLDEIRVIIPKDTQMCE
                     DSISDDEPKSERK"
     gene            1330..2592
                     /locus_tag="ACG8KR_002078"
     CDS             1330..2592
                     /locus_tag="ACG8KR_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0201435.1"
                     /translation="MSEKSIELKEYKRSDGEKYYPVASFSKSVYLVGRALVVGAIGIY
                     ILHWVTKVVQLLVDTLVSILVSLNDVNGTDDLGLKEMFKELLNAFFPNYLSYSYNVQS
                     FFAKLHLVDISILVLEAGTVFFVGYLVTLMFRHHYGEQRPFFDDMESRRLKREALEAM
                     DVKYDPNKIIGDETNNKKRKRNKTQLESDVLSTLKNIKISVHTRRDLSVNEDIREYIV
                     KIPQPKGKKVRETVLRQIQDLGSILTRATSGRAKFGEKEDEVSGDYFIFRASVNIERE
                     RKERVSTKNKETERATSSIAVSNSQFSFPINLFADNSKNIQNQTEKARKYAETQKQSL
                     ELYLSSADVPASLTDMHVGNTSIEYLFTLSRTRKIQLSSMKEEIETYLHISGVIVRIN
                     AGRIQITLPLGENDRIPIDVPSMINSVF"
     gene            2672..4048
                     /locus_tag="ACG8KR_002079"
     CDS             2672..4048
                     /locus_tag="ACG8KR_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HFQ0201436.1"
                     /translation="MAMKLLEKVKKRAEKAESILLDKEPNIKLNGKFSVNMLNTKAKD
                     PTDAIFGIKPDGTIAHFCISEMPHMLVAGTTGSGKSVLLNEILVTVMCHSTPDELKIG
                     IVDPKRVEFGRYKKLPFMLADPITDMDEAYDFFEYLVILMHERYKLMEKAGLQNITLY
                     NEYAEKNGLDRFPYVILLVDEYSQLVGTHKEVEGLIVQLGQMARAAGIHVILATQSPR
                     STVVTGIIKANFPSRVCLMVASDLESRIVLDEGGGESLSPKGDMLIKLVDGSMVRAQG
                     AYISNKEIETIFNHLRNTMPEPEYVDYRAIVAETRGEGGEDEDFGGIGKAPVRKSAKR
                     NLNTPLANPSSKEPSIEKKSAFAIKLEKNKEEKVEQGAKTLEKLQAEKLEKVLRALGK
                     ADPEPQTSNNTNNKQPIASKTKPKANDPKGSENVVITTDKSEKKQINGSTSPIMQKML
                     KAKKQVHK"
     gene            4045..4674
                     /locus_tag="ACG8KR_002080"
     CDS             4045..4674
                     /locus_tag="ACG8KR_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0201437.1"
                     /translation="MIIVSSFLRWLTPYKYTDEVVVKRAKKRDKKAVLTPKQMLDHGI
                     LGVKVLEVNKPFESNSAREKLLGAEADVAYFYHDKSKHQIAIKFNPKSAWRYQARDDL
                     KRRKLRPMLYPEGKEAFDRTHIVPIGFHGTESDNRLLVGWNSNQNRVQINKFEKKVRK
                     FNDRKTILWFADICKQPDGTAKWEAVIFDENGKKVMSETWHDKAKFVWQ"
     gene            4744..5172
                     /locus_tag="ACG8KR_002081"
     CDS             4744..5172
                     /locus_tag="ACG8KR_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0201438.1"
                     /translation="MGETIEYKDKNYIIIGIYNTRINRIGDPRITSDLVCQSVNYSPD
                     LTSRYKKYVLLEYRHKITDEIGVNNTRNIFKIGTVIPYSNNEEKIFYQIYGIEKFEYE
                     HVDLLVTYQMRLIEPWSQAEIDKAVKLNRLSKFNVLGNAF"
     gene            5731..6330
                     /locus_tag="ACG8KR_002082"
     CDS             5731..6330
                     /locus_tag="ACG8KR_002082"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HFQ0201439.1"
                     /translation="MREKNKNSSRYLVATVVFVCVLIFLFDFLQIGKSIIGNTLSAKE
                     MVALGGLYEGHLFPNIFISIVSHINLYHLISNLLFLVVLEEALNDQYEWLTLLIVLII
                     SGFLGSLVPFLLHSNIVSMGLSGGIFGWIALFAIAYIRFDSKKHLLLSVFVLLIIGIL
                     LVSTFESGEANVLSHVIGFVCGMILGFILKNVSLIKQKN"
     gene            6346..6822
                     /locus_tag="ACG8KR_002083"
     CDS             6346..6822
                     /locus_tag="ACG8KR_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0201440.1"
                     /translation="MPKRKEKKIPVFLSVSEMLSLQILLRKKIETIDEEVRNKRAMYE
                     QREALRERIHYFDDIHTKIYEARTNHEDESKLGLAQQRILEWLNTNTDQEPLRVISNL
                     FEFGTKHWFNAVEKDADLQEAFDTLSKDSKSMRDLLITYLNEHEFEQTKTKKKNKE"
     gene            6839..7741
                     /locus_tag="ACG8KR_002084"
     CDS             6839..7741
                     /locus_tag="ACG8KR_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0201441.1"
                     /translation="MLLTLLGYLCLILAFFGAIAIIIGQFQTNNPIMRNIGIGIVIVT
                     FIVFNATKIFGSNDKQFGISGVNNSYAPDSLGRVVIDGTFENVTSYKNVSLTVKEDGK
                     KASDDVLATIKPSQSQFEVFYTVKENSPVHKLTLVFNVDGEEYYKDVKINKTSTKKII
                     ESAKKEVATEESTTESSSTVEAELPDKIDFDPEKVKSFESVSGSMADRFNTEVFKAIK
                     TVKLDTEKQMINVTLFNSVESMDQDKKVTVARELGKRVIGLYRGTAKPDSDGADLPVH
                     VVYEDGKVFGDSTANEALTIKLAK"
     gene            7814..8005
                     /locus_tag="ACG8KR_002085"
     CDS             7814..8005
                     /locus_tag="ACG8KR_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0201442.1"
                     /translation="MVVNKEKVRKYVTLKIASVKYLEKRAKEEERARGKKVTVSELLE
                     ELIENDQTIRRAFTNRSIR"
     gene            8169..8756
                     /locus_tag="ACG8KR_002086"
     CDS             8169..8756
                     /locus_tag="ACG8KR_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0201443.1"
                     /translation="MENNNDIRHFSIGERVTTVSELSKDYRDKEGTIISLNKNRRGVW
                     QYVVDLGDGVTVECKDSQLIRNNGSHSIEKAILVYPKVSSKIMNDWLLGKFGEYNKKE
                     IAEVIGKSYPTVLGYLKKITSKEAKIEDVLTVRELQSLIEYRNKQITNPEYLRKEDGL
                     LKQLEQVNKIRKMQKVAPLSLDTFKKIYLKEIKKD"
     gene            8756..9655
                     /locus_tag="ACG8KR_002087"
     CDS             8756..9655
                     /locus_tag="ACG8KR_002087"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0000400 - four-way junction DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFQ0201444.1"
                     /translation="MWTVTLPFQPVSAPRPSARRNAADETNDVAEKRISTYYDKKYLD
                     YLKKIKSFIEDQDLLDDEFYSIVNDPMGAIMEVDFYYQIPKSYKKVYNIMKTTAPDLD
                     NLVKSAMDGIFNISAIRDSHITGLIAFKYNVANEGKTVVRIKRYSEFEWDTSEGLNGD
                     IWEATFDFKPVATPRPKSKFRGNGIITYYPKEYNDYLENIKNTLKEKDLVNDQLKKVM
                     NAPMGVIAQIDYFYQVRKDKKKLDSLMRTSQPDIDNLVKGTLDGIFNKEIGIKDSRVV
                     GLIAFKYNTFEKSKTNVRLQEMG"
     gene            9655..10569
                     /locus_tag="ACG8KR_002088"
     CDS             9655..10569
                     /locus_tag="ACG8KR_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0201445.1"
                     /translation="MNNNEKILHVLDSFEIIQEELKKYRDVLEQRYDFVNQQKSNHMD
                     FILNMNDLKKKLVERKEQEKLIKAYFELGEKEVKNAMELNEERRVLDQLLEQLLVMFQ
                     KGRIDEDLIEEGLRKYPSNSGIGIVLKAIDEEEIEAFIPPEDFESAMEYIKYYSQGIT
                     AFREFDPEDVIHDLNNLKEWCESYGVDDSGLDYLISIMEIEEEMPDKPDPTDILELIH
                     EARNPIAYISRGYTVLEYYKPYISAMNHLRRVLREKREYRSVLNATNRLEKAVSELDA
                     YYREHYLQAGGMPRNTKANISRYIKKAE"
     gene            10621..10836
                     /locus_tag="ACG8KR_002089"
     CDS             10621..10836
                     /locus_tag="ACG8KR_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0201446.1"
                     /translation="MKRDVFELYVLERLEDGMMAQEIIDEIDDMISRFGQLELNDGNK
                     NSVVLKQFWLNNFSYKDFIKEKANTLY"
BASE COUNT         4269 a         1501 c         2255 g         3089 t
ORIGIN      
        1 aaaatacatt tgacaaaatt tacaatatag tttattatat atttatctta ttatgataac
       61 acaataaaag gaatggtggt atgaaaaaga agaagtttag ccgatcgttg ttaggaattt
      121 tggttttagt tgcggttggt cttagtggca agggttactt tcagaataac agtactcagg
      181 aacatgattt tcagatcaaa agtagtgttc aggtaggtaa taaagcagac agtgaatcaa
      241 aagaattttt agggatacgt tatgatccaa acaaatatcc aaataatatc gtaaacatta
      301 atgatgggaa atatacactg acaaaagaag accatgaagc attatcagaa aaaggcagtc
      361 aaaccttttg ggtaaattat gcagatttag atcaacttgg aagagcagga gatgttgaag
      421 cgttgatcac ctatgatgct gtaaattctc actcatctaa aaatgtggaa cgtccatctt
      481 tcaaaagttc tgtccatatt gcaggagaat acaaggatgg agtatttgat caagagagag
      541 atacttgggt cggcaaagat tcaaacaatg aaatcactca gctagaaggc tataaaggtt
      601 atctctataa caaaagtcat tcattagcat ggtcgctagg aggagatatg gaggtacaca
      661 atttaacact tggaacgaga gcgcaaaatg ttgggaaaaa tgatcagaat ggagggatgg
      721 cttactcaga aacgattgta cgtgatgcaa tctataaaaa taagaatgaa aaagtattgt
      781 ataaagttga tccaatttat aaagggaatg aattagttcc tcgtggttct catgtcgaag
      841 ctttctcagt taacgataat ggaaaaacgg taaaccttaa tgtttatgta tttaacaatc
      901 aaaaaggaat caacatcaac tatcaaaatg gtacttgggt aagagattag aaattgagaa
      961 ggtgaagaat tggaaacaat aagcatcaat ggattagata aatcagttgc aaatcgaata
     1021 aaaaaaggaa atggctacta tcttctgaag aagggtaaat attcagttgc tagagagtta
     1081 gaaacaatga gtatcgtgta tgtagctaag aatcattttg acgtagaaca ggctattttt
     1141 gatccagatt ctgaaatagg gaaatgtgtt gaattatcag agaaaactgt tagtacaaaa
     1201 ggggcgatga tcattgagag agaaatgtta gacgaaatca gagtaatcat tcctaaagat
     1261 acgcagatgt gtgaggacag cataagtgac gatgaaccaa aatcagaaag aaaataagga
     1321 gatattcaga tgagcgaaaa atccattgaa ttaaaggaat ataagcgaag cgatggagaa
     1381 aaatattatc ctgtagccag tttttctaaa tcagtatatc tggttggacg tgcattggta
     1441 gttggtgcaa ttggcatata tattttgcat tgggtaacaa aggttgtaca actgcttgta
     1501 gataccttag taagtattct tgtctcgtta aatgatgtta acggaacaga tgatttagga
     1561 ttaaaagaga tgtttaaaga gttactcaat gcgttttttc ccaattattt aagttattca
     1621 tataatgtac aaagcttttt tgcaaagcta cacttggtag acatctctat tttagtatta
     1681 gaagcaggaa cggtattctt tgttggatat ttggttactt tgatgttcag gcatcactat
     1741 ggagaacaaa gaccattttt tgatgatatg gaaagtagaa ggttgaaaag agaagctcta
     1801 gaagctatgg atgtgaagta tgatccaaac aagattatag gagatgaaac gaacaacaaa
     1861 aaaagaaaac gaaacaaaac gcaattagaa tccgatgtgc tatcaacgct taaaaatatc
     1921 aaaatttctg ttcatacaag acgtgatcta agtgtcaatg aagatattcg agaatatatc
     1981 gtaaaaatcc cgcaaccaaa agggaagaaa gttagagaaa cagttttacg acaaattcaa
     2041 gatttagggt cgatcctgac aagagcaaca agcggacgag ccaaatttgg tgaaaaagaa
     2101 gatgaagttt caggcgatta tttcattttc cgagcatcag taaatattga aagagaacgt
     2161 aaggaaagag taagtaccaa aaacaaagaa acggaaagag caacatcttc aatcgcagtt
     2221 tcaaattcac aattttcttt tccaatcaat ctctttgccg ataattccaa aaatatccaa
     2281 aaccaaaccg aaaaagctag aaaatatgcc gaaactcaaa agcaatcttt agaactttat
     2341 ctatcttctg ctgacgtacc tgcaagtcta acagatatgc acgttgggaa tacatctatt
     2401 gaatatttgt ttactttatc tcgtaccaga aaaattcaac taagctcaat gaaagaagaa
     2461 attgaaactt acttgcatat atcaggtgta atcgtgcgga tcaacgcagg gcgtattcaa
     2521 attactttac cattaggaga aaatgatcgt attccaattg atgtaccatc aatgatcaat
     2581 agcgtgtttt agaagacatc acaaatagaa acagaaaata caaactacca gttcacaaac
     2641 agaaaagtac cgagaaaagt gaggaataga aatggctatg aaattattag agaaagtcaa
     2701 aaagagagcc gaaaaagctg aatctatttt gctagataaa gaacctaata taaagcttaa
     2761 tggaaaattc agtgtcaata tgttgaacac aaaggctaaa gacccaacag atgctatttt
     2821 tggaattaag cctgatggca caattgctca tttttgcatc agcgaaatgc cacacatgtt
     2881 ggttgcagga acgacaggat cagggaaatc agtattactg aatgaaattc ttgttacagt
     2941 aatgtgtcac tcaacgcctg atgaattaaa aataggaatt gtagacccaa aaagggttga
     3001 atttggaaga tacaaaaaat tgccgttcat gttggctgat ccaattacag atatggatga
     3061 agcttatgat ttttttgaat atcttgtgat cttgatgcat gaacgttaca aactgatgga
     3121 aaaagcaggg ttacaaaaca tcacgctata taacgaatat gccgaaaaaa atggattaga
     3181 tagatttccc tatgtgatct tattagttga tgaatattca cagttggttg gaacacataa
     3241 agaagtagaa ggtttgattg ttcaattagg acaaatggca agggcagcag gtattcatgt
     3301 tatcttagca acacaatctc ctcgatcaac agttgtcact ggtattatca aagccaattt
     3361 tccgtcacgt gtttgcctta tggtagcaag tgatttagaa agtaggattg tattagatga
     3421 aggcggtgga gaaagtctaa gtccaaaggg agatatgctt attaagcttg ttgatggaag
     3481 tatggttaga gcgcagggag cttacatcag taacaaagaa attgaaacaa tcttcaatca
     3541 ccttagaaat actatgccag aaccagaata tgttgattac cgagcaatcg tagctgaaac
     3601 tcgtggtgag ggcggtgaag acgaggattt tggtggaatt ggtaaagctc ctgtaagaaa
     3661 atctgctaaa agaaatttga acacaccttt agcaaatcca tcatctaaag agccatcaat
     3721 tgaaaagaag agtgcgtttg ctattaagct tgaaaagaac aaagaagaaa aagtcgagca
     3781 gggagctaaa acactagaaa aactgcaagc agaaaaacta gaaaaagttt taagggcatt
     3841 aggaaaagct gatcctgaac cgcaaacctc aaacaacacg aataataaac agccgattgc
     3901 atctaaaacc aaacctaaag ctaatgatcc aaaaggctca gaaaacgtag tgatcacaac
     3961 tgataaatct gaaaagaaac aaataaatgg aagtacttca cctatcatgc aaaaaatgtt
     4021 aaaagcaaaa aaacaggtgc ataaatgatc atcgtcagtt cttttttaag atggcttact
     4081 ccatataaat atacagatga agtagttgtg aaacgagcga agaaaagaga taaaaaagcg
     4141 gtactaactc ctaaacagat gcttgatcat ggcattttag gggtgaaggt tttagaagtg
     4201 aataaaccgt ttgaatcaaa tagtgcaaga gaaaagttgc ttggagctga agccgatgtc
     4261 gcatattttt accatgacaa aagcaagcat caaatagcta ttaaattcaa tccaaaatcg
     4321 gcatggcggt atcaagcaag agatgacttg aaacgtagga aacttagacc aatgctctat
     4381 ccagaaggaa aagaagcatt tgatagaacc cacatcgttc ccattggttt tcatggtaca
     4441 gaatcagata accgcctatt agtaggttgg aacagcaatc aaaatagagt tcagatcaat
     4501 aaatttgaga agaaagtaag gaaattcaac gatcgaaaaa cgatcctatg gtttgcagat
     4561 atttgtaaac agccggatgg aacagcaaag tgggaagcgg tgatatttga cgaaaatggt
     4621 aaaaaagtaa tgtctgaaac atggcatgat aaagctaaat ttgtttggca gtagcagtag
     4681 aggtgaaaaa gattgctaga actttttgaa gcaaatagaa caggctttaa tcaaaagtta
     4741 gaagtgggag aaactataga atataaagat aaaaactata ttattatcgg aatctataac
     4801 acgagaatta acagaatagg tgatccacgt ataaccagtg acttggtttg tcaatctgtt
     4861 aactattcac ctgatctgac aagcaggtat aagaaatacg tgttattaga atatagacac
     4921 aagattactg atgaaattgg tgtaaacaac acacgaaaca tatttaagat cggaacagtg
     4981 attccttata gtaacaacga agaaaaaata ttttatcaga tttacggaat agaaaagttt
     5041 gaatatgaac atgtcgatct gctagtgacg taccaaatgc gtttaataga accttggtca
     5101 caggcagaaa ttgataaagc agtaaaattg aaccgattat caaaatttaa cgtattagga
     5161 aatgcctttt aaaaatcgca atacacaaac agaaaaatac caaaaaatta gcaatgaacg
     5221 taggtaagaa catgacaata aataataata accctaaaaa atataatgct cttgtccaat
     5281 gctatatcga aaacaaaatt acaatgcaag aagtcgctga tgaattaggc aaatcaaaga
     5341 gcctagtatc acacgatttt aagcacaatg tttttccgaa cccgattgag ctgaggaaaa
     5401 aatttcaaaa gagagtttat caagaaatag gtaagtggat tgatgaaggg aatgcaattg
     5461 accctttaat tgaaaaatat ccttttttaa aaagcaaaaa taaaattcaa aggttaattc
     5521 gtcaaggcga tattcaagct aaagaacctg ttttgacaac caatgatttg gctaaaataa
     5581 atcttcataa acaaattatt gaagactttg aaaaaaatct ttcaacaact gaaattttaa
     5641 aaaaatacaa tattccgaga ggaaagctaa catcgactct ttttagagaa tatggagaat
     5701 cggcagttac taaagcaaag gaagtagcga atgagagaaa aaaacaaaaa tagtagtcga
     5761 tacctagtag cgactgtagt ttttgtttgt gtattgattt tcctgtttga ttttttgcag
     5821 attggaaaaa gcataattgg aaatacattg agtgcaaagg aaatggttgc tttaggtggt
     5881 ttgtatgaag gacacttgtt cccgaatata ttcatttcta tcgtttctca tattaacttg
     5941 taccatttaa tttctaattt attgttctta gtagttcttg aagaagcact gaatgatcag
     6001 tatgagtggt taacattatt gatcgtttta atcatatcag gatttctagg aagcttagtt
     6061 ccttttctgc tacacagcaa catcgtaagt atggggctta gtggaggtat ttttggatgg
     6121 attgctttat ttgcgattgc atacatccgt tttgatagca agaaacattt attactaagt
     6181 gtttttgtat tattgataat cggcatttta ttggtatcta cttttgaatc aggcgaggca
     6241 aatgtcttgt cacatgtaat cggatttgtc tgtggaatga tcttgggatt tattctaaag
     6301 aacgtttcat tgatcaaaca aaagaattaa aagggagtgc taaaaatgcc aaaaagaaaa
     6361 gaaaagaaaa ttccagtgtt tttgtctgtc tcagaaatgt tatcacttca aatactgtta
     6421 agaaaaaaga ttgaaacaat agacgaagaa gttagaaata aaagggctat gtacgaacaa
     6481 cgtgaagcgc taagagaacg aatccactat tttgatgata tacacactaa aatctatgaa
     6541 gcacgtacaa atcacgaaga cgaaagtaaa ttaggattag ctcaacaacg gatacttgaa
     6601 tggcttaata cgaatactga tcaagaaccg ttaagagtga ttagtaatct ttttgaattt
     6661 ggaacgaagc attggtttaa tgctgtagag aaggatgctg acttacaaga agcgtttgat
     6721 acgctaagta aggatagcaa atccatgcga gatttattga ttacttatct aaatgagcat
     6781 gagtttgaac aaacgaaaac caaaaagaaa aataaagaat agaaagttgt gatgaatcat
     6841 gctattaacc ctgttaggtt atttatgttt aattcttgct ttttttgggg caatcgctat
     6901 aattattggt caatttcaaa caaataatcc gattatgcgt aacataggca ttggaattgt
     6961 gattgtaacc tttattgtgt ttaatgccac taagattttt ggaagtaacg ataaacaatt
     7021 tggtatatcg ggagttaata atagttatgc tccagattct ttgggaaggg tagtaataga
     7081 tggaacattt gaaaatgtaa cgagttataa aaatgtttcg ctgacagtga aggaagatgg
     7141 caagaaagca tcagacgacg ttctcgctac tattaagccg tctcaatcac aatttgaagt
     7201 gttttataca gttaaagaga atagccctgt acacaagctg acattggttt ttaacgttga
     7261 tggagaagaa tactataaag atgtaaaaat aaataaaaca tcaacgaaaa agattatcga
     7321 atcagcgaaa aaagaagtag ctacagaaga aagtacaact gaatcaagtt ctactgtcga
     7381 agcggaacta ccagataaaa ttgattttga tcctgaaaaa gtcaaatcgt ttgaatctgt
     7441 ttctggttct atggctgatc ggtttaacac tgaagtcttc aaagctatca aaactgttaa
     7501 attggatact gaaaagcaaa tgattaatgt taccttattc aattcagttg aatctatgga
     7561 tcaggataaa aaagttacgg ttgcaagaga actaggaaaa agagtaattg gtttgtatcg
     7621 tggtactgct aaaccagatt cagacggtgc agatttgcca gttcatgtag tttatgaaga
     7681 cggtaaagtt tttggagatt ctacagcaaa cgaagcatta acgattaaat tggctaaata
     7741 gccagaaagt tataataaac aaatggtgtc acaccaataa tatataggtg tgacaccttg
     7801 gagatgatag ctgatggtag taaataaaga aaaagttaga aagtatgtga ctttaaaaat
     7861 agcttctgtt aaatatttag aaaagcgagc taaagaagaa gaaagagcaa gaggtaagaa
     7921 agtaacagtt tcagagttgt tagaagaatt gatcgagaat gatcaaacaa taagacgtgc
     7981 atttactaat aggtctatca gataacaata taattaataa aaaagaaaga gtgatttggt
     8041 cttagactaa atcactcttt ttatataaag tttctttaca ctcattccga ataatgttaa
     8101 aattctttat atgatatata gttccttgtc agaggaagag ataaccagca atattggagg
     8161 gataataagt ggaaaacaat aatgatatta gacattttag cattggggaa agagttacaa
     8221 cagtaagtga attatcaaag gattatagag ataaagaggg aacgattatt tctcttaata
     8281 agaatcgaag aggcgtttgg caatatgttg ttgatttagg tgatggcgtt acagttgagt
     8341 gtaaagatag tcaactgatc agaaataatg gcagtcattc cattgaaaaa gcaattcttg
     8401 tatacccaaa agtatcaagt aagatcatga atgactggtt gcttggaaaa tttggtgagt
     8461 acaataaaaa agaaatcgca gaagtgatcg gcaaatctta tccaacagta ttagggtatc
     8521 taaaaaagat aacgagtaaa gaagcaaaaa ttgaggatgt tttaacagta agagagcttc
     8581 aatcattaat cgagtatcgg aataaacaaa taacaaatcc tgagtacctt agaaaagaag
     8641 atggtctatt gaaacaattg gaacaagtaa ataaaatccg gaagatgcaa aaggtagctc
     8701 ctttaagcct tgataccttt aaaaaaatct atttgaaaga gatcaaaaaa gactaatgtg
     8761 gactgtaact ttaccgttcc aacctgtatc tgcaccaaga ccaagtgcaa gaaggaatgc
     8821 tgctgatgaa actaatgacg tagcagagaa aaggatttca acctactatg ataaaaagta
     8881 tcttgattat ttgaaaaaaa taaagagctt tatcgaagat caagatttat tagatgatga
     8941 attttactcg attgtaaatg atcctatggg cgcaattatg gaagtggact tttactacca
     9001 gataccaaaa agctataaaa aagtctataa cattatgaag acaacagcgc cagatttaga
     9061 taatttagta aaaagtgcta tggatggtat ttttaacatc tcagctatac gagatagcca
     9121 tatcacaggc ttaattgctt ttaaatataa tgtggcgaac gaaggaaaaa cagttgtgag
     9181 gatcaaacga tattcagaat ttgagtggga tacttctgaa ggattgaacg gagatatatg
     9241 ggaagctaca tttgatttta aaccagtagc aacgccaaga ccaaagtcta aatttagagg
     9301 aaatgggatt atcacttact atccaaaaga gtacaacgac tatttggaaa atatcaagaa
     9361 tacgcttaaa gagaaagatt tggtaaatga tcaactgaaa aaggtaatga atgctcctat
     9421 gggggttata gctcagatag attattttta ccaagtgaga aaagataaga aaaagcttga
     9481 ttcactgatg cgtacttcac agccggatat tgataattta gtcaaaggaa cattggacgg
     9541 aatttttaac aaggaaatag gcatcaaaga tagcagggtt gtagggttaa ttgcttttaa
     9601 atacaatact tttgaaaagt cgaaaacaaa cgtgagacta caggagatgg gctgatgaat
     9661 aataatgaaa aaattcttca tgtacttgat agctttgaaa taattcaaga agaactaaaa
     9721 aaatatcggg atgttttgga acaaagatat gattttgtca atcagcaaaa gagtaatcac
     9781 atggatttca ttctcaatat gaatgacttg aagaagaaat tagttgaacg aaaagaacaa
     9841 gaaaagttga tcaaagctta ctttgaactc ggtgaaaaag aagttaaaaa tgcaatggaa
     9901 ctgaatgaag agcgcagggt tttagatcag ctgttagaac aattattggt gatgtttcaa
     9961 aaaggacgaa tagatgagga tttaattgaa gaaggattga gaaaatatcc ttcaaactca
    10021 ggtatcggaa tagtgctgaa agcgattgat gaagaagaaa ttgaagcttt cataccgcca
    10081 gaggattttg aatcggcaat ggaatatata aaatattata gccaagggat cactgctttt
    10141 agagagtttg atcctgaaga tgttattcat gacttgaaca acctaaaaga atggtgtgaa
    10201 agttatggag tcgatgattc cggattggac tacttgatca gtattatgga aatcgaagaa
    10261 gagatgccgg ataagcctga tccaacagat attttagaat tgatccatga agctagaaat
    10321 ccaattgcct atatttctag aggatatact gtattggaat actataagcc ttatatatca
    10381 gcgatgaacc atttaagacg tgtattaagg gaaaaacgtg aataccgctc agtgttgaat
    10441 gcaacgaata gattagaaaa agctgtttca gagctagatg cttattatcg tgaacattac
    10501 ctgcaagcag gtggtatgcc aagaaatacg aaagcaaata tcagtaggta tataaaaaaa
    10561 gctgagtagg cggagttagc ccataaaatg gcaaaaggta acctgaaagg ggcattttga
    10621 atgaaaaggg atgtttttga attgtacgta ttagaacgtc tagaagacgg catgatggca
    10681 caagagataa ttgatgaaat tgatgatatg atcagcagat ttggtcaatt ggagttgaat
    10741 gatggaaaca aaaattcggt agtgctaaaa caattttggt tgaacaattt ttcgtataag
    10801 gattttatca aagaaaaggc aaatacattg tactgactaa attcacgagg agcagtgatg
    10861 agagcttatt gctaagagca gttaggctct tttttgtttg tgtaaaatta tttgttttat
    10921 aatacacatt tatacacaaa aaaatattga cagatagtac acaaatgtgg taggatatat
    10981 ttgtaagcaa gttagattaa caagttaaag gaagagcagt tatgaaaaaa gaagaactaa
    11041 tagatatatt tcaaattgtt gaacgagcaa ataatatggg aattatgttt tttgatcgta
    11101 tttctttaaa gatg
//
LOCUS       DAZKLD010000100        10877 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.100, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000100 DAZKLD010000000
VERSION     DAZKLD010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10877)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10877)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10877
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.100"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            185..1345
                     /locus_tag="ACG8KR_002090"
     CDS             185..1345
                     /locus_tag="ACG8KR_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HFQ0201447.1"
                     /translation="MKITEKNVNPKFEPTESNLKKFDLTVTLSNELVDSIVEMDAMPQ
                     KIYLYAIGHLDPKNPPFNNTVKVSKKALFALMSSSSSSSKHSRLKQVIEKLHQQSVIK
                     IQRTKPNGKTEYKVISPLEITEFNDFSDAVSIKFSSSIMPLLTNLEDHFTQYAFDQVV
                     NLTSKYAISFFQLFTKDFNLYLANRDNLTWSTKSLDRLKNPVFSIADLRKKFSIEKKY
                     KAFRDFERRVIKEPIAQLNKYTDFKIDYELIKKGHTYTNIKFLLTRTMSVPVPYKQEQ
                     RDPAYLKGAEDKTEKMNQSYLKAMNSKYTKLLIKNFLISSADLCDLKLMSRLQNKVYP
                     LYDRLSKDRGFESVVNHLSYVATHKEDYTIKNTVKYLEKSITSYLPYVNLYD"
     gene            1380..2147
                     /locus_tag="ACG8KR_002091"
     CDS             1380..2147
                     /locus_tag="ACG8KR_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HFQ0201448.1"
                     /translation="MKKDELTKYNEEFLATIDMKMDLKSYELYQLLFSYISPTKLKKT
                     GVVDLPVKAIINYMDVDQNLETELKTIVNTLQRQAVYEISNLDKKYKTYTTVSAIDRI
                     HWDLEEHSVSFSFSEDVIPLIVSIKKKYPFFVFEDLASFQKKYSIVLYKYLAMYYKKY
                     LIAKNKGDFEKAEIYKNPLIEIVKIREITKTENEYKTISNFDRRVLRESVEEINEKSM
                     LNIDFAKVRKGRNYNFAVQFNISSEFDEKTYREKKLG"
     gene            2339..2737
                     /locus_tag="ACG8KR_002092"
     CDS             2339..2737
                     /locus_tag="ACG8KR_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HFQ0201449.1"
                     /translation="MKYFFCAVFEWKGNEYAVKFPDFPDLYTHGETLEDAFEMAQEVL
                     EGYLVLLELQEKEIPSPTPVQELNARIKRSENVNLFVVKANTKVAKARNKQNYDRKSV
                     TVEHYLNVLAAEENINLSKALNDLLREKLI"
     gene            complement(3044..3418)
                     /locus_tag="ACG8KR_002093"
     CDS             complement(3044..3418)
                     /locus_tag="ACG8KR_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0201450.1"
                     /translation="MKQTQTETVNDTLADQLAGYYKLFEMGKAQRLIHYLWKYKILFL
                     FWESTLTFSVLFYKSDFYNQHLVLGSFLLIYTILMLNLYTYITLEEHLVISDAGIERN
                     IKRVSFKITLNKPTDKKLSEAF"
     gene            complement(3440..3790)
                     /locus_tag="ACG8KR_002094"
     CDS             complement(3440..3790)
                     /locus_tag="ACG8KR_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0201451.1"
                     /translation="MKATHLNYFSRTLINNLLKDTTGISVSTKHFANDLIIFEDNNLR
                     IEMFIEQATPNSIPEFCYTHTEKMKHAKSVKDVEALSESLTNALNLVKKAKLLFEFQT
                     DYHNELLAQSEILD"
     gene            complement(3807..4202)
                     /locus_tag="ACG8KR_002095"
     CDS             complement(3807..4202)
                     /locus_tag="ACG8KR_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HFQ0201452.1"
                     /translation="MKTKNIFYPAIFEKEGEAYNIVFPDLPEISTFGNSLEEAYLNAK
                     DALGLALYSVPDAEFPKPSAVEELTLKANQVVVIIQLNIKLFRRSLNTKTIRKNVSVP
                     EWLVLLGKEKNINFSQLLQKALEEELLEK"
     gene            complement(4269..4454)
                     /locus_tag="ACG8KR_002096"
     CDS             complement(4269..4454)
                     /locus_tag="ACG8KR_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HFQ0201453.1"
                     /translation="MPKTAREIVKILKSNGFKKTGQTGSHAKFYNSKTKRTVVVPIHN
                     GDVPLGTEANIWKQAGL"
     gene            complement(4536..4790)
                     /locus_tag="ACG8KR_002097"
     CDS             complement(4536..4790)
                     /locus_tag="ACG8KR_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0201454.1"
                     /translation="MNYTDEEQITLEYVFIDEEGEEIEKVLIISSKDLENALTYEPYE
                     STVTVNEFLDTYTSDDTWEIMNICNANNIQFAEKTNKSFY"
     gene            complement(4812..5057)
                     /locus_tag="ACG8KR_002098"
     CDS             complement(4812..5057)
                     /locus_tag="ACG8KR_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0201455.1"
                     /translation="MWTLEGKTFAEIWTEFIEVPTRIDPITSEKVLDADFYNLKAGTS
                     LECVWMYLEEHFKGVTLGHVLYHNGAEWDAIGNIHFS"
     gene            complement(5064..5348)
                     /locus_tag="ACG8KR_002099"
     CDS             complement(5064..5348)
                     /locus_tag="ACG8KR_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0201456.1"
                     /translation="MLTATLQQPTHVAIAESYTINAGIVKQLIKDREFKKFNSRQVYN
                     RQLKKAILKHKDSFIGNLESVDVKTVSTAFSVVEVTIDGRLISLFAFGIN"
     gene            complement(5451..5633)
                     /locus_tag="ACG8KR_002100"
     CDS             complement(5451..5633)
                     /locus_tag="ACG8KR_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HFQ0201457.1"
                     /translation="MINLFLLVTASTLFFTGISLIISYLIYKKAENHTSKATGLAVSS
                     FLTLLTLFVTLSFSFV"
     gene            complement(5839..6324)
                     /locus_tag="ACG8KR_002101"
     CDS             complement(5839..6324)
                     /locus_tag="ACG8KR_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0201458.1"
                     /translation="MGWYGDYSSANEVIEEKKGYAKNGLEIVDTKVTKIYGIILFKDA
                     EGTYDIDSFIFRDEMYKPIYFLNDSPAYFKKIPKKWLKFFVGDQEKMVKRQLDRIKEE
                     SNKPKLDDLLVAGKTYSIWDGSYVAVYQYKLKRSFIFRLQDGSLTRFTRLSAGDVKEV
                     A"
     gene            complement(6491..6757)
                     /locus_tag="ACG8KR_002102"
     CDS             complement(6491..6757)
                     /locus_tag="ACG8KR_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFQ0201459.1"
                     /translation="MTYQVRYEREAQKALKKMDKFQAKVILNWIEKNLVGTDDPRKHG
                     KGLTANKSGYWRYRVGAYRIIADISEHEVTILILSIGHRSEVYK"
     gene            complement(6759..6980)
                     /gene="relB"
                     /locus_tag="ACG8KR_002103"
     CDS             complement(6759..6980)
                     /gene="relB"
                     /locus_tag="ACG8KR_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB family
                     antitoxin"
                     /protein_id="HFQ0201460.1"
                     /translation="MPVISIRVSKEEKDFLEKVAEFNGDNISEFMRSNSLQTAETLVD
                     FATYKQLMKEHEENDQSISHEDMLKELGL"
     gene            7206..8024
                     /locus_tag="ACG8KR_002104"
     CDS             7206..8024
                     /locus_tag="ACG8KR_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFQ0201461.1"
                     /translation="MEKKLSIITGNIEKGGTGKTTTIYNLGGYAVENLGMKVLYIDED
                     KSQNLSKRFPDAFSKAKRENTINHLYLHGSAEPLAINENMDILVAGEDLKQVEMQMRD
                     MPNNRTLLFRWIVVNYEQLKEKYDLILIDTHNDAGLLAQNAWAVSDLVVGISDPSKDG
                     FEALLKLGKDIDALAKQLIDIKTGETLMETKYATIGSRIKHNTNSSREFLELVPTLSN
                     YLGYVQEKELLNATSLEGKNIAEFAKDKNIYRKNKDFFESTFTLYNKIIDTAQE"
     gene            8037..8324
                     /locus_tag="ACG8KR_002105"
     CDS             8037..8324
                     /locus_tag="ACG8KR_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0201462.1"
                     /translation="MKENRFKKFAEAEVEEVPEVVEAVEEPKQTPKETTSSLVAKLKS
                     DSSEKKKNYQFTLQPSQRKKLSKISEKLGIRSDSALLGEMIDNLYDEVFGE"
     gene            complement(8506..8859)
                     /locus_tag="ACG8KR_002106"
     CDS             complement(8506..8859)
                     /locus_tag="ACG8KR_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0201463.1"
                     /translation="MNKGKSERYVVIINREKVSELPLAEFLESEYETLGVKYSQTIKK
                     ALNLYRRVKNNEVTIIEAGNDRNDSKVAEVKKKPTDHNPIQQEHPSTVTSDHSAKKEK
                     PMLKGFNAPSNKDLL"
     gene            complement(8879..9934)
                     /locus_tag="ACG8KR_002107"
     CDS             complement(8879..9934)
                     /locus_tag="ACG8KR_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0201464.1"
                     /translation="MKEPLLFSVDLGNKSVKEMSTHVSPLSLPSRILNKNIINSGRFS
                     NIGSSLKRSLNVSEYSLVNTTESYYFGKDIHELGKDDYVIESLGFGISRYKQETYKNI
                     LSFSLANLARLEDQSDDVLTVNLVLGLPSSDYTETIVKYVMQQATRQHSVEVDGKAYN
                     INVDTVWVLPQPIGTLYNLLLADDGSVQDETLISQKIGFVDIGGGTKLFDVVQNFEID
                     ENNREQKDTGIFTLYNRIVNMLDLPISPNAFQLEKIIREGKEKGVYLFKPSGNESFDI
                     SEVVEREIKTYTNMVVAEVLATFKNFTTLDKVVFTGGGASIIDKQLVKDKIPHTLFVE
                     DSEFSNVKGFYKFGLTQ"
     gene            10150..10548
                     /locus_tag="ACG8KR_002108"
     CDS             10150..10548
                     /locus_tag="ACG8KR_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0201465.1"
                     /translation="MRLRRNKNYTTIILEDFTDQELLIVGKTKADFIEKGNASIVKAS
                     KVAVEKYFDSLEESAKEIFNGEKAGSYELDGIRFSLIPTTENIIPKKLYYIFSSEKHT
                     SMKPCIFDWQWLIDAVLDVKCSKYNITLGC"
     gene            10551..10724
                     /locus_tag="ACG8KR_002109"
     CDS             10551..10724
                     /locus_tag="ACG8KR_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0201466.1"
                     /translation="MSMLVRKLPLSVREFLFYLVCLIASGFKLIEHIFIFAYVLAMIA
                     FLSGGWLIIYLLS"
BASE COUNT         3605 a         1702 c         1865 g         3705 t
ORIGIN      
        1 ctgtcttatc aatgaaaatt gattaaaaaa ataaaatgtg ttttacagag attgagttat
       61 gttataaatt tacgatacat ttcttattgt atcgtatcga aaacaggtct atacttattc
      121 aatggaatcg taccagttcc aaatttttaa gctcgtacca agcttagaaa gaagaggcaa
      181 tcgtatgaaa ataacagaaa agaatgtaaa cccgaaattt gaacctaccg aaagcaatct
      241 aaaaaagttt gatttaactg ttactttatc gaatgaactt gtagacagca ttgttgaaat
      301 ggatgctatg ccacaaaaaa tttatcttta tgctatagga catcttgacc ctaaaaatcc
      361 gccttttaat aatactgtta aagtatctaa aaaggcttta tttgcgttga tgtcttctag
      421 ttcatcttct tcaaaacatt caagattaaa acaagttatt gaaaaattgc atcagcaatc
      481 tgtcattaaa attcaaagaa ctaaacctaa cggtaagact gaatacaaag tcatctcacc
      541 gctagaaatt actgaattca atgatttttc tgatgcagta tctattaaat ttagtagttc
      601 aattatgcct ttattaacga acttagaaga tcattttacg caatatgcat ttgatcaagt
      661 agttaacttg acaagcaagt atgcgatcag tttttttcaa ttattcacta aagattttaa
      721 tctgtattta gcaaatagag ataacttaac atggagtaca aaatcacttg atagattaaa
      781 aaatcctgtt ttctccattg cagatttacg aaagaaattt agtattgaga aaaaatataa
      841 agcttttcga gactttgaaa gacgtgtaat taaagaaccg attgcccaac tgaataaata
      901 cactgatttt aaaattgact atgagcttat caagaagggg catacttata caaatataaa
      961 gttcttgctt acacgtacca tgtcagttcc ggtaccttat aagcaagaac aaagagaccc
     1021 tgcttattta aaaggcgcag aagataaaac agagaaaatg aatcaatcgt acttgaaagc
     1081 tatgaatagc aaatacacta aattactaat caaaaacttt ttgatcagtt ctgccgactt
     1141 atgcgatctt aaacttatga gccgacttca gaataaagtt tatccgcttt atgatcgttt
     1201 atccaaagat agaggatttg aatcagttgt caatcaccta tcttacgttg ctactcataa
     1261 ggaggattac acaataaaaa atactgttaa atatctcgaa aaatcaatta cgagttattt
     1321 gccttatgtg aacctttatg attaacaact gaaaaaatgc gtgaaggaag gaaaaacttt
     1381 tgaaaaaaga tgaactgaca aagtacaatg aggagttctt agcaacaatt gacatgaaaa
     1441 tggatttaaa gtcgtatgaa ctttatcaat tgctgttcag ttatatctca cccacaaagc
     1501 taaagaaaac aggcgtagta gatttacctg ttaaagcgat tattaattac atggatgttg
     1561 atcaaaatct tgaaacggaa ctaaaaacca tagttaacac tttgcaaaga caagcagttt
     1621 atgaaatttc gaatctagac aaaaaatata agacgtatac tactgtatct gctattgatc
     1681 ggattcattg ggatttagaa gagcatagtg tatctttcag tttttcagaa gatgtaattc
     1741 ccttaattgt ctcaatcaaa aagaagtatc cgttctttgt ttttgaagac ttagcatctt
     1801 ttcaaaaaaa atatagcatc gtgttataca aatatttggc gatgtattat aaaaaatatc
     1861 ttatcgccaa aaataaagga gattttgaga aagctgaaat atataagaat cccctgattg
     1921 aaatagttaa aattcgtgag attacgaaaa cagaaaatga gtataaaaca atatcgaatt
     1981 ttgatagacg tgttttaagg gaatcagttg aggaaattaa tgaaaaatca atgctgaata
     2041 ttgattttgc taaagttaga aaaggcagaa attacaattt tgctgtccag tttaatattt
     2101 ctagtgagtt tgatgaaaaa acttatagag aaaaaaaatt aggttaatgc aaaatatatc
     2161 ttactcgttt gaagatgctt cagacgtatg ctaagatata tagtgccttg ggagcaaggt
     2221 attaatctcc tttagaggtt aattagcttg tgatcgccta tcaatttttt tggtaggcga
     2281 ttcttttttt tgaaacaaaa aagcaaaaat tattttttaa ttagagaggt gcaatgcgat
     2341 gaaatacttt ttttgtgcag tattcgaatg gaaaggaaat gaatatgcag ttaaattccc
     2401 agattttcca gacttatata cacatggtga aactttagaa gatgcttttg agatggcgca
     2461 agaagtattg gaaggatact tggtgctttt agagttgcaa gaaaaagaaa ttccatcgcc
     2521 tacgcctgtt caagagttaa atgcaagaat caagcgatct gagaatgtta atttatttgt
     2581 cgtaaaagct aatacgaaag ttgctaaagc tcgaaacaag caaaattatg atcgaaaatc
     2641 agtgactgtg gaacactatt taaatgtttt agcggctgaa gagaatatta atctgtcgaa
     2701 agctttgaat gatttgttga gagaaaagtt gatttgatgt ctctcagatg atcatattaa
     2761 gcaaaaacag cttgttttag gacgtatgga tttaagaaag acaaattatc aaagaatata
     2821 ttaaaagctc tcagaagcct tctgagagct tttaaattgc tatgtattgt actgcatctg
     2881 ctcaaattga ttttcttttc atgcttacca tgtatctaat tttccatcaa gtaattttta
     2941 gatatgtatt gagtaataaa tagcaaattt gcttgatata agctcgaatt aggtgctttg
     3001 taatgtgtta ggataaaatt atacaagaaa atgtcaaaag ctctcagaac gcttctgaga
     3061 gctttttatc ggtaggcttg tttaacgtga tcttgaagga tacccttttg atgtttctct
     3121 cgatccctgc atcgctaatt actaagtgtt cttcaagtgt gatgtaggta tataaattaa
     3181 gcatcaaaat ggtgtagata agcaaaaaac tccctaaaac taagtgttga ttataaaaat
     3241 ctgacttata aaaaagaact gagaatgtta gtgttgattc ccaaaaaaga aacaaaattt
     3301 tgtatttcca aagatagtgg atcaagcgct gtgctttacc catctcaaat aatttataat
     3361 aacctgctaa ttgatcagca agcgtatcgt taacggtttc tgtttgtgtt tgtttcatct
     3421 ttctcttctc ctgtttcaat taatctaaaa tttcagattg agcaagcaac tcattgtggt
     3481 agtcggtttg aaattcaaat aacaattttg ccttcttaac gaggtttaaa gcgttcgtta
     3541 gtgattcact taatgcttca acatctttaa ctgattttgc atgtttcatt ttttcagtgt
     3601 gcgtatagca gaattcaggt attgagttag gggttgcctg ttcaataaac atctcaattc
     3661 gcaagttatt atcctcaaaa ataattagat cattggcaaa gtgtttggta ctaacgctaa
     3721 tgccagttgt atcttttaat agattattga ttaacgttcg agagaaatag tttaagtgtg
     3781 tagctttcat aggttcggtc tcctttttac ttttcaagca actcttcttc cagtgctttt
     3841 tgaagtagtt gagagaagtt tatgtttttc tccttcccta aaagaactaa ccattccgga
     3901 acactaacat tttttctaat tgtttttgta tttaagcttc ttcggaaaag tttaatgttt
     3961 agttggatga ttactaccac ttggttggct ttcaaagtta attcttccac tgctgatggt
     4021 ttaggaaatt ctgcatctgg tacactatat agcgctaatc ctaaagcatc cttagcgttt
     4081 aaatatgctt cttcaagact atttccaaag gtagatattt ccggtaagtc agggaaaacg
     4141 atgttataag cttctccttc tttctcgaag attgccggat aaaaaatatt ttttgtcttc
     4201 atttcctcgc tccttttcta gtgtaagaga ggatagtatg cctaatacca aataggaagg
     4261 caaggctgtt aaagccctgc ttgcttccag atattagctt ctgttcctag tggtacatca
     4321 ccattgtgga tcggaacaac gaccgttctt ttagtcttgg aattataaaa ttttgcatga
     4381 ctgccagttt gtccagtctt tttgaagccg ttagatttga gaatttttac aatttctctt
     4441 gctgtcttag gcatagctac cctcccttta caaaatctat tatacacatt gttgtgtatg
     4501 gaatcaatag aaaaagagga tgttttattg caaaactaat aaaatgattt attggttttt
     4561 tcagcaaatt gaatgttatt agcgttgcaa atattcatga tttcccaagt atcatcagaa
     4621 gtataggtat ctaagaattc attgacagtt acggttgatt cgtatggttc ataagtcaaa
     4681 gcgttctcca agtcttttga tgaaataatc aagacttttt caatttcctc cccttcttca
     4741 tctatgaaaa catattctaa agtgatttgt tcttcatcgg tataattcat attgtgttct
     4801 cctttcttgt actaagaaaa atggatattt ccaattgcat cccactcggc accattgtgg
     4861 tataggacgt gaccgagcgt tacgcctttg aaatgttctt ctaaatacat ccaaacgcat
     4921 tcaaggctag tacctgcttt gagattgtag aagtctgcat ccaatacctt ttcagaagtt
     4981 atcggatcaa tgcgagttgg tacttcaata aattcagtcc aaatttctgc aaaagtctta
     5041 ccttccaaag tccacactcc ctatcaatta attccaaaag caaatagaga aatcaagcga
     5101 ccgtcaatag tcacttcaac aactgaaaat gccgttgaaa cagttttaac atctacactt
     5161 tccaagttcc cgatgaagga atctttgtgc tttagaatag ctttctttag ctgtctgtta
     5221 tacacttgcc gactgttaaa ctttttgaat tctcgatcct tgatcagctg tttaacgatc
     5281 cctgcattaa ttgtataact ttctgcaata gcaacgtgag taggttgctg taaagtagct
     5341 gttagcatct tgtcactcct tcctaaaagt taattagctt gtttacaaat acattataca
     5401 catcggtgtg tataatgtaa agagaaaaag gtgctaatgg atcagctgtt ctaaacaaaa
     5461 gaaaacgata aagtaacaaa taaagtcagc aaagttaaaa aggatgaaac ggctagtccg
     5521 gttgctttgc ttgtgtgatt ttcggctttc ttatagatca aataggaaat tatcagtgat
     5581 attccagtaa agaataatgt actagctgtc actaaaagaa ataggttaat catacgatac
     5641 actccttctg taatattggt atcagatacg tttaaatgcg tttctgagct gttttggatc
     5701 agatggtgat gtttttagcc ctaccctaaa aacaaagccc agaaaagaat ttaaaagcgt
     5761 tcgtacctaa tacgtttata ttcatataca tatacataaa cataaacgtg tatacgttta
     5821 tgtttatgta tttaagtttt aggctacttc cttaacatct cctgctgata gtcgagtgaa
     5881 ccgagtgagt gatccatctt gtagtcgaaa aataaagcta cgtttgagtt tatactgata
     5941 gacagcgaca tacgatccat cccagattga gtaagtttta cctgcaacta ataagtcatc
     6001 aagttttggt ttattgcttt cttctttaat tctatctagt tgtcgcttaa ccattttttc
     6061 ttgatcaccg acaaagaatt tcaaccattt tttcggaatt ttcttaaagt aagcaggact
     6121 gtcatttaag aagtagattg gtttgtacat ttcatctcga aagatgaagc tgtcaatatc
     6181 gtaggttcct tctgcatctt tgaaaaggat aattccatag atcttcgtta ctttggtatc
     6241 tacgatttcc aaaccatttt tggcgtaccc tttcttttct tcgatcactt catttgctga
     6301 tgaataatca ccataccatc ccataaaaaa tcgctccttt tgaaagttaa ttagcttgtt
     6361 tacacttata ttatacacac cggtgtgtat aatatcaaga ggatattgtt cttttttagg
     6421 taaagtggga tcgtaaacag tgattttgtt gggttaacaa aaaaacagac aagccttatc
     6481 agcttgtctg ttacttataa acttcacttc tgtgaccaat agacaatata agaattgtca
     6541 cttcatgctc gctaatatca gctattatac gatatgcacc taccctatat cgccagtagc
     6601 ctgatttatt agctgttaga cctttaccat gctttcttgg atcatcagtg cctacaagat
     6661 ttttctcgat ccagtttagg ataacctttg cctgaaactt atccattttt tttagcgctt
     6721 tttgagcttc acgttcatat cttacttggt aagtcatctt ataagcctaa ctcttttagc
     6781 atatcttcgt gggaaatact ttgatcgttt tcctcatgct ctttcattag ttgcttgtag
     6841 gtggcaaagt ctactaacgt ttctgctgtt tgcagggaat tactacgcat aaattcagaa
     6901 atgttatctc cgttgaattc agcaactttt tcaagaaaat ctttttcttc tttagataca
     6961 cgaatactta ttactggcat agtttatcaa ctccttttca attacattgt actacgattg
     7021 tatgacaaat gcaatagtta tatatttgaa tatatacata aacataaaca tatacatata
     7081 tatgtttatg ttgtagaata taaaagcaaa tataaatata aacacgttta tgtatataaa
     7141 catatacata aacgtgttta tagtgtataa tggagttaca aaaggttaat aagggaggtt
     7201 taatcatgga aaagaagttg tcaatcatta ctggaaacat tgaaaaaggt ggaacaggta
     7261 aaacaacaac aatctacaat ctaggtggat atgctgtcga aaatttaggt atgaaggtgc
     7321 tgtatattga tgaagataag tcgcaaaacc tatcaaaacg atttcctgat gctttcagta
     7381 aagcgaaaag agagaataca atcaatcatt tatatcttca tggatcagct gaacctttag
     7441 ctataaatga gaacatggat attttagtag caggtgaaga tttaaaacaa gttgagatgc
     7501 aaatgcgtga tatgcctaac aacagaaccc tactgtttag atggatcgtt gttaactatg
     7561 aacagcttaa agagaagtat gatctaattc tgattgatac tcataatgat gcaggtctac
     7621 tagctcaaaa tgcttgggct gtatctgatc ttgtcgtagg catttctgat ccatctaaag
     7681 atggttttga agctttgctg aagcttggta aagatattga tgcactagca aaacagttga
     7741 ttgatattaa gactggtgaa acactgatgg aaactaaata cgctacgatc ggtagcagga
     7801 tcaaacataa tactaattca agtcgtgagt tcctagaatt ggtaccaacg ttatctaatt
     7861 atcttggata tgtacaagag aaggaattgc ttaatgcaac tagcttagaa ggtaagaata
     7921 ttgctgagtt cgctaaggat aagaatatct atcgtaagaa taaggatttc tttgaaagca
     7981 cgtttacgct atacaacaaa ataattgata cggcacaaga ataaggagtg attgaagtga
     8041 aagaaaatag atttaaaaaa ttcgctgaag ctgaagtaga agaagttcct gaagtggttg
     8101 aagcagttga agaacctaaa caaacaccta aagaaacaac aagttctttg gtagctaaac
     8161 taaaaagtga tagttctgaa aagaagaaaa attatcaatt cacgcttcaa ccatcacaaa
     8221 gaaagaaatt atctaaaatc agcgaaaagt taggaattcg atcagattca gctttacttg
     8281 gtgaaatgat agataacttg tatgatgaag tttttggtga gtgatcagtt taaagctgat
     8341 cacttttttt attggaataa gcggttaggt atagcttaac taaatttcgt atacgtgttt
     8401 atggatatat acgtatacat gtttatatac atgtatacgt atatgtttat gtttgtattt
     8461 gtacataata aaaaggctag gtattgtaac ctcgcctttt tatttttaaa gtaagtcttt
     8521 gttacttggt gcattgaacc cttttagcat tggcttttct ttctttgctg aatgatcgga
     8581 tgtaaccgtt gaaggatgct cttgttgaat agggttatga tcagttggtt tcttctttac
     8641 ttctgctact ttgctatcgt ttctatcgtt gcctgcttca ataattgtaa cttcattgtt
     8701 ctttacacgc cgatataggt tcaaagcttt tttgatcgtc tgagagtatt taacgcctag
     8761 tgtttcatat tcggattcaa gaaactctgc taaaggtaac tcgctaacct tttctctatt
     8821 gattataact acataacgct ctgattttcc cttattcatg ttgatctcct tcgctgtttt
     8881 attgagttaa accaaacttg taaaagcctt ttacattaga aaattcactg tcttcaacaa
     8941 acaaggtatg cggaatttta tcttttacaa gttgtttgtc gataatagaa gctccaccac
     9001 cagtaaagac aactttatct aaggttgtga agtttttgaa tgtagctaaa acttctgcaa
     9061 caaccatatt ggtgtatgtt tttatttctc tttcaactac ttctgaaata tcaaaacttt
     9121 catttccgct tggcttaaat agatatacgc ctttttcttt gccttctcgg ataatctttt
     9181 caagttggaa agcattagga ctgatcggaa ggtcaagcat atttacgata cgattatata
     9241 aagtaaaaat gccggtatcc ttttgttcac gattgttttc gtcaatttca aaattttgga
     9301 caacatcaaa aagtttcgtg ccaccaccaa tatcaacaaa accgatcttc tgactgatca
     9361 atgtttcatc ttgtacacta ccatcatcag caagtaggag gttatatagt gttccaatcg
     9421 gctgtggcag tacccaaacg gtatctacgt ttatattata agccttaccg tcaacttcaa
     9481 ctgaatgttg tcgtgtggct tgttgcatca cgtatttgac gattgtttcg gtatagtcgg
     9541 atgaaggcag acctaaaact agattaacgg ttaaaacgtc atcagattga tcttctaaac
     9601 gtgctaggtt agctaaagag aaagatagga tatttttgta ggtttcctgc ttgtatctgc
     9661 tgatcccaaa acctagtgat tctatcacgt agtcatcttt tcctagctcg tgtatatcct
     9721 tcccaaaata atagctttca gtagtgttta ctaggctata ttcagaaacg tttagtgatc
     9781 gttttaatga ggaacctata ttagaaaaac gtcctgaatt aatgatattt ttgttcaaaa
     9841 tacgagaggg aagtgagagt ggagaaacgt gtgtactcat ttcttttact gatttattcc
     9901 ctaagtcaac ggaaaacaat aatggttctt tcatggtatg acctctttct gtttgtgttt
     9961 atggtatttc ggtacgaaaa tagtttacac ctatttacta ttagttgcaa ctgtttataa
    10021 gaaaaaaatg caatcataaa agtctaatta attataataa agtgtaaata aaaaggtgta
    10081 aataaatgct tgcaaattta tcttattatg atattataaa atcataaatt atattaagga
    10141 gtgatcctga tgcgattgag aagaaataag aattacacta ccattatttt agaagatttt
    10201 actgatcaag aattacttat tgtcggtaaa acaaaagcag attttatcga aaagggaaat
    10261 gcttcaatag taaaagcttc aaaggttgct gtcgaaaaat attttgattc tttagaagaa
    10321 agtgctaagg aaatttttaa tggagagaaa gctggtagct atgagttaga cggtattaga
    10381 tttagcttga tcccaacaac tgaaaatatt attccgaaga aattatatta tatcttttca
    10441 tctgaaaaac atacttcaat gaagccttgt atttttgatt ggcaatggtt gattgatgct
    10501 gtattggatg taaaatgttc aaaatacaat attactttgg ggtgttaatt atgagtatgc
    10561 tagttagaaa attaccatta tctgtaagag aatttctatt ttacttggtc tgcttaatcg
    10621 catcagggtt taagctgatc gaacatattt ttatctttgc ttatgtatta gcaatgatcg
    10681 catttttatc tggcggttgg ttaattattt atttgttaag ctaagtttta gatcaaaaat
    10741 aattagtaaa ttaaacgata aaataaccgt taataaatgt aatgaaagga caacgcctaa
    10801 caatcagctt gtaataagat tataaaataa cataatatca aaaatagtgc tttttaaagg
    10861 acaaattaag tgtatca
//
LOCUS       DAZKLD010000101        10769 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.101, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000101 DAZKLD010000000
VERSION     DAZKLD010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10769)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10769)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10769
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.101"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            78..193
                     /gene="rrf"
                     /locus_tag="ACG8KR_002110"
     rRNA            78..193
                     /gene="rrf"
                     /locus_tag="ACG8KR_002110"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            207..279
                     /locus_tag="ACG8KR_002111"
     tRNA            207..279
                     /locus_tag="ACG8KR_002111"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:240..242,aa:Val,seq:tac)
     gene            283..355
                     /locus_tag="ACG8KR_002112"
     tRNA            283..355
                     /locus_tag="ACG8KR_002112"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:316..318,aa:Lys,seq:ttt)
     gene            363..444
                     /locus_tag="ACG8KR_002113"
     tRNA            363..444
                     /locus_tag="ACG8KR_002113"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:397..399,aa:Leu,seq:tag)
     gene            462..534
                     /locus_tag="ACG8KR_002114"
     tRNA            462..534
                     /locus_tag="ACG8KR_002114"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:495..497,aa:Thr,seq:tgt)
     gene            546..617
                     /locus_tag="ACG8KR_002115"
     tRNA            546..617
                     /locus_tag="ACG8KR_002115"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:578..580,aa:Gly,seq:gcc)
     gene            634..719
                     /locus_tag="ACG8KR_002116"
     tRNA            634..719
                     /locus_tag="ACG8KR_002116"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:668..670,aa:Leu,seq:taa)
     gene            730..803
                     /locus_tag="ACG8KR_002117"
     tRNA            730..803
                     /locus_tag="ACG8KR_002117"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:764..766,aa:Arg,seq:acg)
     gene            813..886
                     /locus_tag="ACG8KR_002118"
     tRNA            813..886
                     /locus_tag="ACG8KR_002118"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:847..849,aa:Pro,seq:tgg)
     gene            906..978
                     /locus_tag="ACG8KR_002119"
     tRNA            906..978
                     /locus_tag="ACG8KR_002119"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:939..941,aa:Ala,seq:tgc)
     gene            1006..1079
                     /locus_tag="ACG8KR_002120"
     tRNA            1006..1079
                     /locus_tag="ACG8KR_002120"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1040..1042,aa:Met,seq:cat)
     gene            1093..1166
                     /locus_tag="ACG8KR_002121"
     tRNA            1093..1166
                     /locus_tag="ACG8KR_002121"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1127..1129,aa:Ile,seq:cat)
     gene            1190..1279
                     /locus_tag="ACG8KR_002122"
     tRNA            1190..1279
                     /locus_tag="ACG8KR_002122"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1226..1228,aa:Ser,seq:tga)
     gene            1292..1365
                     /locus_tag="ACG8KR_002123"
     tRNA            1292..1365
                     /locus_tag="ACG8KR_002123"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1326..1328,aa:Met,seq:cat)
     gene            1384..1459
                     /locus_tag="ACG8KR_002124"
     tRNA            1384..1459
                     /locus_tag="ACG8KR_002124"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1417..1419,aa:Asp,seq:gtc)
     gene            1461..1531
                     /locus_tag="ACG8KR_002125"
     tRNA            1461..1531
                     /locus_tag="ACG8KR_002125"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1493..1495,aa:Gly,seq:tcc)
     gene            1545..1618
                     /locus_tag="ACG8KR_002126"
     tRNA            1545..1618
                     /locus_tag="ACG8KR_002126"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1579..1581,aa:Ile,seq:gat)
     gene            1643..1730
                     /locus_tag="ACG8KR_002127"
     tRNA            1643..1730
                     /locus_tag="ACG8KR_002127"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1677..1679,aa:Ser,seq:gct)
     gene            1752..1823
                     /locus_tag="ACG8KR_002128"
     tRNA            1752..1823
                     /locus_tag="ACG8KR_002128"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1785..1787,aa:Glu,seq:ttc)
     gene            1846..1935
                     /locus_tag="ACG8KR_002129"
     tRNA            1846..1935
                     /locus_tag="ACG8KR_002129"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1882..1884,aa:Ser,seq:tga)
     gene            1948..2021
                     /locus_tag="ACG8KR_002130"
     tRNA            1948..2021
                     /locus_tag="ACG8KR_002130"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1982..1984,aa:Met,seq:cat)
     gene            2040..2112
                     /locus_tag="ACG8KR_002131"
     tRNA            2040..2112
                     /locus_tag="ACG8KR_002131"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2073..2075,aa:Asp,seq:gtc)
     gene            2116..2188
                     /locus_tag="ACG8KR_002132"
     tRNA            2116..2188
                     /locus_tag="ACG8KR_002132"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2149..2151,aa:Phe,seq:gaa)
     gene            2193..2263
                     /locus_tag="ACG8KR_002133"
     tRNA            2193..2263
                     /locus_tag="ACG8KR_002133"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2225..2227,aa:Gly,seq:tcc)
     gene            2276..2349
                     /locus_tag="ACG8KR_002134"
     tRNA            2276..2349
                     /locus_tag="ACG8KR_002134"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2310..2312,aa:Ile,seq:gat)
     gene            2361..2432
                     /locus_tag="ACG8KR_002135"
     tRNA            2361..2432
                     /locus_tag="ACG8KR_002135"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2394..2396,aa:Glu,seq:ttc)
     regulatory      2621..2700
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01767"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="SMK box riboswitch (SAM-III) riboswitch; SAM
                     riboswitches modulate the expression of downstream genes
                     in response to levels of S-adenosylmethionine; SMK box
                     riboswitch shares no known similarity to SAM-I and SAM-II;
                     Derived by automated computational analysis using gene
                     prediction method: cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF01767"
     gene            2698..3891
                     /gene="metK"
                     /locus_tag="ACG8KR_002136"
     CDS             2698..3891
                     /gene="metK"
                     /locus_tag="ACG8KR_002136"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HFQ0201467.1"
                     /translation="MVERHLFTSESVSEGHPDKIADQISDAILDAILKQDPTARVACE
                     TSVTTGLVLVFGEISTTAYVDIQKVVRETIKEIGYTRAKFGFDGDTAAVLVAIDEQSP
                     DIAQGVDEALEIRDEDKKDVLDEIGAGDQGLMFGFAVDETPELMPLPIALSHRLVRRL
                     ADLRKSNELTYLRPDAKSQVTVEYDDQGQPERVDTIVISTQHDDAVDNETIRHDVIEK
                     VVKEVIPAELLDDQTKYYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLARKAEVQLAYAIGVAQPVSISINTFGTGTVP
                     EEELIAAVRENFDLRPAGIIEMLDLRRPIYKQTAAYGHFGRTDVDLPWEHTDKVDALK
                     ASLAK"
     gene            4021..5493
                     /locus_tag="ACG8KR_002137"
     CDS             4021..5493
                     /locus_tag="ACG8KR_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HFQ0201468.1"
                     /translation="MKKETNVTLVTVSIFIATFMTAIEGTIVTTAMPTIVGSLHGMEI
                     MNWVFSIYLLTNAMLTPIYGKLADKVGRKPIFIFGTLLFIIGSLFCGLSQSMIGLIAA
                     RAIQGMGAGAMMPVALTIIGDLYSIEKRAKVLGLNSSAWGIASVFGPLAGGFIVETIS
                     WHWIFFINVPIGILLIILIWLYLNEPKIIHESKPMDIFGSFSLMAMLVSLLLGFQLLG
                     DEGMSPLVSALLLGSIVMLGIFVFAEKRAKDPVIMMHLFKNPLFVVVNLAAALASGFL
                     MGIDVYIPMWMQGVLGLNAAIGGLVLAPLSLVWMVGSFLASKWMTQMKTHWVLRIGLL
                     IAAIGGAWLYLMPFRSNYLWFFAISTVIGIGLGVTMTTSTVSAQNSVSRDQLGVATSF
                     NTLVRTIGQTVMVSIFGLLINGVTKRELAAANLTNDADIMNKLVNPQTAKYIDADILL
                     PLRKILYDGLHSVYLVGLLLVIIALVLTLFVHEKSGKIKQ"
     gene            5576..6364
                     /locus_tag="ACG8KR_002138"
     CDS             5576..6364
                     /locus_tag="ACG8KR_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFQ0201469.1"
                     /translation="MAFIQANIYSNVLEMEVNLNVILPQTTKKVIGTSSEQETTDIPV
                     LYLLHGMGGNHSVWERRTSIERYVANYGLAVIMPSTDLGWYTDTVYDMKYWTFIAEEL
                     PEICHELFPQLTRKREKTYAAGLSMGGYGALKLGLAKSENFAAVISLSGAVSVGNRMD
                     DLLMVRKASFWEGIFGPLDQIEGSKNDPQYLLKELVKSGKPVPRFYMACGESDFLYQA
                     NQEMVSAMQEQGLAVTFEQGPGDHNWIFWDQWIQRALAWLMEEK"
     gene            complement(6431..7099)
                     /locus_tag="ACG8KR_002139"
     CDS             complement(6431..7099)
                     /locus_tag="ACG8KR_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HFQ0201470.1"
                     /translation="MEIKLSQAIIIQAAFDILAETQALSQLSMRNIANRIHVQAPALY
                     WYFKNKQQLLQKMAETMEEELALPDPTLPWNEQLLQYMENYYDLYTRFPCGAELEINT
                     VPAFPSRLEHLEQMIQLLVREGFSVTQSHQAIASLHNLLIGQLMDQQHEQQLRQKIMD
                     GNEFLKETVLFMRNYVVENQLEGFKESMQSHAELNEKALFLTSAQTYIEGLCQQNKRR
                     ENEQ"
     gene            7214..8926
                     /locus_tag="ACG8KR_002140"
     CDS             7214..8926
                     /locus_tag="ACG8KR_002140"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0201471.1"
                     /translation="MKLILRHAKKYKVAVFISLLSVAVMVAAALWQPKLLQQVLEAII
                     TEDNDEMKTIGIYLISLALLGLAAGVTNTIFSAKVAQGVSADIREEAFRKIQTFSFGN
                     IEQFSAGNLVVRLTNDITQIQNLVMISLQSLFRIPFLFIGAFILAMITMPQLWWIIVA
                     LIVTVFLITALSFTRMGKHFMIIQKLIDRVNSIAKENLLGIRVVKSFVQEENQLKSFS
                     KVSEDLTKHNIIVGTLFSVMIPSFMLAANLAVVGAIFFVSDLVKDDPTLIGGIASFMN
                     YLMQIMMAIIIGGMMMMMTSRAAVSLKRIAEILDAEPDLTYLDVPEQELTGSVTFDHV
                     SFRYPGEDTDTLKDISFSIKPGEMVGIVGATGAGKSTLAQLIPRLFDPTEGKVEVGGV
                     DLRQVNEKSLRQTVSFVLQKAILFSGTIAQNLRQGKKNATEKEMEHASSIAQAKEFIE
                     KLSDRYEAPIEERSSNFSGGQKQRLSITRGVIGNPKILILDDSTSALDARSEKLVREA
                     LDRDLKGTTTIVIAQKIASVVKADRILVLDEGRLVGEGTHEELVAGNRVYQEIFETQK
                     GTEE"
     gene            8929..10701
                     /locus_tag="ACG8KR_002141"
     CDS             8929..10701
                     /locus_tag="ACG8KR_002141"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0201472.1"
                     /translation="MTDLIKASKFFYHYLKRYKLSFFFIFITVIIATYLQVKAPQYIG
                     EAFQELANYVGGLMQGVDDKSKFVSVIWKLLLFYVLASAANFIYSILFTQVVGKSTNR
                     MRIGLFNKLEKLTIRFFDSHQDGEILSRFTSDLDNIQNSLNQALLQVITNAVLLVGIL
                     IMMFRQNVELAWATIASTPVAVLIAVLIIRKARKYVDLQQDEVGKLNGYMDEKISGQR
                     VIITNGLQEETIEGFLEHNQSVREATFKGQAYSGLLFPMMQGMSLVNTAIVIFFGGWL
                     ALNGDLERSVALGLVVTFVQYSQQYYQPLMQISSGYSMIQLAITGARRLNEIFDEKDE
                     VKPENGKAFDGVEKGLALQHVDFGYSPEKLVLKDVSINVDKGEMVALVGPTGSGKTTI
                     MNLLNRFYDVNDGSVVIDGIDIRDYDLDALRRHVGIVLQESVLFSGTIKDNITFGKPD
                     ASDEEVINAAKQANIHDFIMTLEKGYDTEISEENNLFSTGQKQLISIARTIITNPSLL
                     ILDEATSNVDTVTEARIQKAMDEAIKGRTSFVIAHRLKTILNADRIVVLRDGEVIEEG
                     SHHELLAENGFYAELYHNQFVFES"
BASE COUNT         3095 a         1966 c         2500 g         3208 t
ORIGIN      
        1 actcggaaaa aaacttatgt gacttcaaat ccagttttga gtgaacaaaa tttactcaat
       61 aaaagataac accacagtgt ggtggcgata gcgagaagga tacacctgta accatgccga
      121 acacagaagt taagcttctt agcgccgatt gtagtgaggg gttgcccctt gtgagagtag
      181 gacgtcgcca cgcttaagtt atccatggag gtttagctca gctgggagag catctgcctt
      241 acaagcagag ggtcagcggt tcgatcccgt taacctccat atgagccgtt agctcagttg
      301 gtagagcatc tgacttttaa tcagagggtc acaggttcga gccctgtacg gctcatattt
      361 ttgcgggtgt ggcggaattg gcagacgcac tagatttagg atctagcgcc ttacggcgtg
      421 ggggttcaag tcccttcacc cgcataattt aagttttaca tgccggctta gctcagttgg
      481 tagagcatct gatttgtaat cagagggtcg agggttcaag tcctttagcc ggcatcttta
      541 aacacgcgga aatagctcag tggtagagca ccaccttgcc aaggtggggg tcgcgggttc
      601 gaaccccgtt ttccgctttg ccaaagcaat cgcgccgggg tggcggaact ggcagacgca
      661 caggacttaa aatcctgcgg tgagtgatca ccgtaccggt tcgattccgg tcctcggcat
      721 ttcaaatatg cgcccatagc tcaactggat agagtgtttg actacggatc aaaaggttag
      781 gggttcgact cctcttgggc gcgttaatta aacgggaagt agctcagctt ggtagagcac
      841 ttggtttggg accaaggggt cgcaggttcg aatcctgtct tcccgataat tataaattta
      901 aacacggggc cttagctcag ctgggagagc gcctgctttg cacgcaggag gtcagcggtt
      961 cgatcccgct aggctccatt tcatttaaat aatgaatatc tttttcgcgg tgtagctcag
     1021 ctggctagag cgtccggttc atacccggga ggtcgggggt tcgatcccct ccgccgcgat
     1081 ttttaagagc ctggaccttt agctcagttg gttagagcag acggctcata accgtccggt
     1141 cgtaggttcg agtcctacaa ggtccattgt ggcgttgtaa gtttcatatg gaggattacc
     1201 caagtccggc tgaagggaac ggtcttgaaa accgtcaggc gggtaaaacc gtgcaagggt
     1261 tcgaatccct tatcctcctt tttcatatta tcgcgggatg gagcagtctg gtagctcgtc
     1321 gggctcataa cccgaaggtc gtaggttcaa atcctgctcc cgcaattaaa aactaaaaac
     1381 actggttccg tggtgtaggg gttaacatgc ctgcctgtca cgcaggagat cgcgggttcg
     1441 attcccgtcg gaaccgccat gcgggtgtag tttagtggta aaaccacagc cttccaagct
     1501 gttgtcgcga gttcgattct cgtcacccgc ttttagtttt ttatgggcct atagctcagc
     1561 tggttagagc gcacgcctga taagcgtgag gtcgatggtt cgagtccatt taggcccatt
     1621 agtacaaaat cttattggtt ttggggaagt actcaagtgg ctgaagaggc gcccctgcta
     1681 agggtgtagg tcgggaaacc ggcgcgaggg ttcaaatccc tccttctccg tttcaatatt
     1741 ttgttttacc tggcccgttg gtcaagcggt taagacaccg ccctttcacg gcggtaacac
     1801 gggttcgaat cccgtacggg tcattaattt aatatagttt tacatggagg attacccaag
     1861 tccggctgaa gggaacggtc ttgaaaaccg tcaggcgggt aaaaccgtgc aagggttcga
     1921 atcccttatc ctcctttttc atattatcgc gggatggagc agtctggtag ctcgtcgggc
     1981 tcataacccg aaggtcgtag gttcaaatcc tgctcccgca attaaaaact aaaaacattg
     2041 gttccgtggt gtaggggtta acatgcctgc ctgtcacgca ggagatcgcg ggttcgattc
     2101 ccgtcggaac cgtttggctc ggtagctcag ttggtagagc aatggattga agctccatgt
     2161 gtcggcagtt cgattctgtc ccgcgccatt atgcgggtgt agtttagtgg taaaaccaca
     2221 gccttccaag ctgttgtcgc gagttcgatt ctcgtcaccc gcttttagtt tttatgggcc
     2281 tatagctcag ctggttagag cgcacgcctg ataagcgtga ggtcgatggt tcgagtccat
     2341 ttaggcccat ttattttatt ggcccgttgg tcaagcggtt aagacaccgc cctttcacgg
     2401 cggtaacacg ggttcgaatc ccgtacgggt catggaagat actttttgag tatctttttc
     2461 tttttatctg aaagaatgat tttaaaaagc ttagcagagt tatctttatg gatgattgtg
     2521 ctaagctttt ttggacagtt ctaatgtaaa atatttttat attttttaag caaaaaaatg
     2581 aaaaaatacg agcagaaaac tgttttatct gctatacttg gttcgttgca agttcccgaa
     2641 aggatccgca ggacggagat gccttgtaac cgaatttaac tagggggaat cattataatg
     2701 gtagaaagac acttatttac atcagaatct gtttctgaag gacatccaga taaaattgct
     2761 gaccaaatca gtgatgcaat cttggatgca attttaaaac aagatccaac agcacgagta
     2821 gcgtgtgaaa cgtctgtaac aactggtctt gttttagtat ttggagaaat atctacgacg
     2881 gcatatgtag atattcaaaa agtcgtacgg gaaacaataa aagaaattgg ttatacacgt
     2941 gcaaaattcg gatttgatgg agatacagca gctgtattgg ttgcgattga tgaacaatct
     3001 cctgatattg ctcaaggagt tgatgaagca cttgagatcc gggatgaaga taaaaaagac
     3061 gtattagatg agattggtgc tggtgaccaa ggtttaatgt ttgggtttgc tgtagatgaa
     3121 acaccagaat tgatgccttt accaatcgct ttgagtcatc gtctggtacg acgcttggca
     3181 gatttgcgta aatcaaatga attaacgtat ttacgtccag atgcaaaatc tcaagtaacg
     3241 gttgaatatg atgatcaagg acaaccggaa cgcgtagata caatcgttat ttcaacacag
     3301 catgatgatg cagtagataa tgaaacaatt cgtcatgatg tcatcgaaaa agtagtgaag
     3361 gaagttattc cagctgaatt attagatgat caaacgaaat attatatcaa tccgactggc
     3421 cgatttgtca ttggtggtcc tcaaggggat gccggattaa caggaagaaa aatcattgtt
     3481 gatacgtatg gcggttatgc tcgtcatggt ggcggtgctt tttctggtaa agatgcgaca
     3541 aaagttgacc gttctgccag ctatgctgca cgttatattg ctaaaaacat tgttgcagca
     3601 gggcttgctc gcaaagcaga agtacagcta gcttatgcga ttggtgttgc tcaacctgtt
     3661 tcgatctcaa tcaatacttt cggtactgga acggttccgg aagaagaact gattgctgca
     3721 gtgagagaaa acttcgatct tcggccagca ggaatcatcg agatgcttga tttgcgtcgt
     3781 ccgatttaca aacaaacagc tgcttacggt cattttggtc gtacagatgt agatttgcct
     3841 tgggaacata cggacaaagt agatgcgttg aaagcaagtt tagcaaaata agttatattc
     3901 agctggaacg atcgccaagt ggtggttgtt ccagcttttt ttatggcagt taacctcttg
     3961 aaaaagatag taattattta tctattttta gatcctgtca taatgaaagg gaggaataag
     4021 atgaaaaaag aaacaaatgt tactttggtc acagtgagta ttttcattgc aacgttcatg
     4081 acggctattg aaggtacaat cgtaacaacc gctatgccaa cgattgtcgg ttcattacat
     4141 ggaatggaaa tcatgaactg ggtattttcg atttatttgc tgacaaacgc tatgcttact
     4201 ccgatttacg gaaaacttgc ggataaagtg ggacggaaac caatctttat ttttgggaca
     4261 ttattgttta ttatcggttc gttattttgc ggtttatctc aaagcatgat tgggttgatt
     4321 gctgctcgtg cgattcaagg gatgggtgct ggggcaatga tgccagtagc tttgacgatc
     4381 atcggcgatc tttattctat tgaaaagcga gcaaaagttt taggtttgaa tagttcagcc
     4441 tgggggattg ccagtgtttt tggaccgctt gcgggaggat tcatcgttga aacgatcagc
     4501 tggcactgga tcttttttat caatgtgccg atcggaattc tattaattat tttgatttgg
     4561 ctatatttaa atgaaccaaa aatcatacat gagtctaaac caatggatat ttttggtagt
     4621 tttagtttga tggccatgct tgtctcattg ctgttagggt tccaattatt aggagacgaa
     4681 ggaatgagtc cgcttgtttc tgctttgctg ctaggtagta tcgtaatgtt aggtattttt
     4741 gtttttgccg aaaaaagagc taaagatcct gtcatcatga tgcatttatt caaaaatcca
     4801 ctatttgttg tggtcaactt ggctgcagca ttggctagtg gttttttgat gggaatcgat
     4861 gtttatatcc ctatgtggat gcaaggtgta ttagggctga atgcagcaat tggtgggctg
     4921 gtattagcac cattatcact agtctggatg gtcggttctt tccttgcaag caagtggatg
     4981 actcaaatga aaactcattg ggtcttgaga attggactac taatcgcagc tattgggggc
     5041 gcttggttgt atcttatgcc tttcagaagt aactatttat ggttttttgc tatatcaacg
     5101 gtcatcggta taggcttagg tgtgaccatg actacctcga ctgtcagtgc acaaaatagt
     5161 gtatctcgtg accagcttgg tgtagcaact tcttttaata cgttagttcg aacaattggt
     5221 caaacagtca tggtttctat ttttggttta ttgataaatg gggtaactaa acgagaactt
     5281 gcagcagcaa atttaacaaa tgatgcagat atcatgaata aactggtgaa tccacaaacg
     5341 gctaaatata ttgatgcgga tattttgtta ccattaagaa aaattcttta tgatgggtta
     5401 cattccgttt atctagttgg cttgttactc gtgatcattg ctttggtctt aacgcttttc
     5461 gttcatgaaa aaagtggtaa aataaaacag tagtttcggt taagatatgt acgtagccga
     5521 atcattcaaa tttaaagtta ttcaaaaata acttcttact aaggaaggcg aaaatatggc
     5581 atttattcaa gcaaatattt attcgaatgt gttagaaatg gaagtcaatc taaatgtgat
     5641 actaccgcaa acaacaaaaa aggtgattgg caccagttca gagcaagaga caacggatat
     5701 acctgtttta tatttacttc atggaatggg tggcaatcat agtgtatggg aaagacgtac
     5761 gtcgattgaa cgttatgtag caaactacgg attagctgtc atcatgccat ctactgatct
     5821 tggctggtac acagatacag tttatgacat gaagtattgg acattcattg cagaagagct
     5881 acctgagatt tgtcatgaat tattcccgca attaacaaga aaaagagaaa aaacatatgc
     5941 cgctggatta tcgatgggcg gatatggcgc gttaaaacta ggccttgcaa aatcagaaaa
     6001 tttcgcagca gtaatctctc tttcaggcgc tgtttctgta ggaaatcgaa tggatgatct
     6061 tttgatggtt cgtaaagcat ctttttggga agggatcttt ggaccattgg atcaaatcga
     6121 aggttcaaaa aatgatccgc aatacttatt gaaagaacta gtaaaaagcg gaaaaccagt
     6181 tccaagattt tatatggctt gtggggaatc tgattttctt tatcaggcaa atcaggaaat
     6241 ggtatctgct atgcaggaac aaggcttggc ggtcaccttt gaacaaggac ctggcgatca
     6301 taactggatc ttctgggacc aatggattca acgtgcattg gcgtggctaa tggaagaaaa
     6361 ataaaaaaag agtcttaaaa agatagctcc tatagagatc acaaacatag tgatctctat
     6421 aggagctttt ttactgctcg ttttctcttc ttttattttg ttggcataag ccttcaatat
     6481 aggtttgtgc actcgttaga aagagtgctt tttcattcaa ttctgcatgg gactgcatac
     6541 tttctttaaa tccctctaat tgattttcaa ctacataatt acgcataaat agaactgttt
     6601 ctttcaaaaa ttcatttccg tccataattt tttgtcgaag ctgctgttca tgctgctgat
     6661 ccattaactg tccaatcaat aaattgtgga gagaggcaat ggcctgatga ctttgggtga
     6721 ctgaaaatcc ttcacgaaca agtaattgga tcatttgctc caaatgttct aatcgactcg
     6781 gaaaagcggg cacagtattg atttctaatt ctgccccaca agggaaacgt gtataaaggt
     6841 catagtaatt ttccatatac tgcagcaact gttcattcca aggcaaagtt ggatctggaa
     6901 gcgctaattc ttcttccatt gtttcagcca ttttttgcaa gagctgttgt ttgtttttga
     6961 aataccaata taaagcaggt gcttgaacat gaattctgtt tgcgatgttt cgcatggaaa
     7021 gctgcgaaag agcttgtgtt tcagccagga tatcaaaggc ggcctgaatg atgattgctt
     7081 gagaaagctt tatttccatt ttatcaacct cgtgtattgc attatctttt taaacatggt
     7141 aaattgttag tcggtctaat tttacagtgt taaattagtc ctgtcaattt aaacaaaggg
     7201 gtgtaatcaa cgaatgaaat taatcttacg acatgcgaaa aaatataaag tagctgtttt
     7261 tatttccttg ctgtccgtag cagtcatggt tgcagcagca ttatggcaac ctaagttact
     7321 acagcaagta ttggaagcta ttattacaga agataatgat gaaatgaaaa caattggtat
     7381 ttacctgatc agtctggctt tgctaggatt agccgcaggt gtaacgaaca cgattttctc
     7441 tgcaaaagta gctcaaggcg taagtgcaga tatccgagaa gaagcatttc gaaagatcca
     7501 gacattttca tttggaaata tcgaacagtt ctctgctgga aatcttgttg ttcgtctaac
     7561 aaatgatatc acgcaaatcc aaaatttagt gatgatttct ttgcaatcac tttttcgaat
     7621 tccattctta tttattggtg ccttcatttt agctatgatc acgatgccgc aattatggtg
     7681 gatcatcgta gcgcttatcg ttacggtatt tctgattacg gcactttcat ttacaaggat
     7741 gggcaagcac ttcatgatca ttcaaaaact gattgatcgt gtgaatagca ttgcaaaaga
     7801 aaacttacta gggattcgtg tagtgaaatc ttttgtacaa gaagaaaacc aattgaagtc
     7861 attcagcaaa gtaagtgagg acttaacaaa acacaacatc atagtcggta cattattttc
     7921 tgtgatgatc ccgtccttca tgctggctgc caatttggct gtggttgggg cgatcttttt
     7981 tgtgagcgat cttgttaaag atgatcctac gttaattggt ggaattgctt cattcatgaa
     8041 ctacttgatg cagattatga tggcaatcat tattggcgga atgatgatga tgatgacttc
     8101 aagagcagcc gtatcgctga agcggattgc ggaaatttta gatgctgaac cagatcttac
     8161 ttatttagac gttccagaac aagagttgac gggttctgtc acttttgacc atgtttcttt
     8221 tcgttatcct ggtgaggata cagatacgct taaagatatc agcttttcta tcaaacctgg
     8281 agaaatggtt ggtatcgtcg gggcgactgg tgccgggaag tcaacactag cacaattgat
     8341 ccctcgtcta tttgatccga cagaaggcaa agttgaagta ggtggtgtag atcttcgtca
     8401 agtaaatgaa aagagtttaa gacaaacggt ttcctttgtt ttgcaaaaag caattttatt
     8461 ctcaggaacg attgctcaaa acttgcgcca agggaaaaag aatgcaacag aaaaagagat
     8521 ggaacatgct tcttctattg ctcaagcaaa agaattcatc gaaaagttat ctgatcgtta
     8581 cgaagcacca atagaagaac gtagcagtaa cttttctggt ggtcaaaagc aacgtttatc
     8641 cattactcgt ggcgttatcg gtaatccaaa gatcttgatc ctagatgaca gtacgagtgc
     8701 actggatgct cgctctgaaa agctggtccg agaagcactc gatcgagatt tgaagggaac
     8761 aacgacgatt gtcatcgcac aaaaaattgc ttctgttgtc aaagcagacc gcatcttagt
     8821 attggatgaa ggcagattag tcggcgaagg aacccatgag gaattggtag ctggaaaccg
     8881 tgtctatcag gaaatctttg aaactcaaaa aggaacggag gagtaagtat gactgatcta
     8941 ataaaagcaa gtaaattttt ctatcactat ttgaagcggt acaaactctc ctttttcttt
     9001 atctttatca cagtgatcat tgctacctat ctacaagtaa aggcacctca atacatcggg
     9061 gaagcttttc aagaattagc taattacgta ggaggcttga tgcaaggcgt agacgataaa
     9121 agcaaatttg tttctgtaat atggaaactc ttgctgtttt atgtactagc tagtgcagcc
     9181 aactttatct atagtatttt gttcacacaa gtagtaggga agtcaacgaa ccgaatgcgt
     9241 atcggtctat tcaacaaatt ggaaaaattg acgatccgtt tctttgattc tcatcaagac
     9301 ggtgaaattt taagtcgttt taccagtgac ttagataata ttcaaaatag tttgaaccaa
     9361 gctttgcttc aagtcatcac gaatgctgtt ttacttgtcg ggatcttgat catgatgttc
     9421 cgccaaaatg tagagttagc ttgggctaca attgcttcca ctccagtagc cgttttgata
     9481 gctgtcttga ttatccgcaa agcacggaaa tatgtggatc ttcagcaaga tgaagtgggg
     9541 aaactaaatg gttatatgga tgaaaaaatc agcggacaac gtgtcatcat cacaaatggc
     9601 ttacaagaag aaacgattga aggattctta gagcataatc aaagtgttcg cgaagcaacc
     9661 ttcaaaggac aagcttattc tggtctacta tttccgatga tgcaaggaat gtcactggtc
     9721 aatacagcga tagttatctt tttcggcggc tggttagcat taaatggtga tttggaacgt
     9781 tcggtggcat taggcttagt tgtgacattc gtccagtatt cacaacaata ttaccaacca
     9841 ctgatgcaga tttcatctgg atacagcatg atccaattag cgattacagg ggcaagacga
     9901 ttgaatgaaa tatttgatga aaaagatgaa gtcaaaccag aaaacggtaa agcatttgat
     9961 ggtgttgaaa aaggtcttgc gttgcagcac gtagattttg gttattctcc agaaaaacta
    10021 gtcttgaaag atgtctcgat caatgtggac aaaggagaaa tggttgctct tgttggtcca
    10081 acaggatcgg ggaaaacgac gatcatgaat ctgctaaacc gtttttacga tgtcaatgat
    10141 ggttcagtag tcatcgatgg aatagatatc cgagattatg atcttgatgc attacgtaga
    10201 catgtaggaa tcgttttgca agaatctgta ttgttctccg gaacgatcaa agacaatatc
    10261 acattcggca aaccggacgc ttcagatgaa gaagtcatca atgcagcaaa acaagcgaat
    10321 attcatgact tcatcatgac gttagaaaaa ggttatgata cagagatatc cgaagaaaat
    10381 aacttattca gtacaggtca aaagcagttg atcagtattg ctcgaacgat catcacaaat
    10441 ccatctttat tgattttgga tgaagcaaca agtaatgtag acacggtaac agaagcaagg
    10501 atccaaaaag caatggatga agcaatcaaa ggaagaacca gctttgtgat tgctcaccgg
    10561 ctgaaaacaa tcttgaatgc agatagaatc gttgtcttgc gtgatgggga agtcattgaa
    10621 gaaggcagtc atcatgagtt attagcagaa aatggatttt atgcggaact ttatcataat
    10681 caatttgtat ttgaaagtta aattaaaaga acctcgttat aacggggttc tttttatata
    10741 ttttatgttg tttgttttta taaggggca
//
LOCUS       DAZKLD010000102        10540 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.102, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000102 DAZKLD010000000
VERSION     DAZKLD010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10540)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10540)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10540
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.102"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            23..646
                     /locus_tag="ACG8KR_002142"
     CDS             23..646
                     /locus_tag="ACG8KR_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0201473.1"
                     /translation="MFVIWEVVKSRVQEMNEHFSVSFAENPSKYFRRLVFLLILSLLL
                     TVAMFLLPEKIREEQVNSLVYSSIPTSKLKPFDYSKADQKIKQSRAISVMFSKPNGKT
                     YQDMLDVFNDPELMEELNRPIFYYPIVYDVHELEQKYNIRTDEVTFIFFDGGKEANRI
                     TAGKGGITDFDKELIPELNRLPLANIKQLEEELDRTGVTSQNSEIAN"
     gene            1096..2145
                     /gene="trpX"
                     /locus_tag="ACG8KR_002143"
     CDS             1096..2145
                     /gene="trpX"
                     /locus_tag="ACG8KR_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan ABC transporter substrate-binding
                     protein"
                     /protein_id="HFQ0201474.1"
                     /translation="MKNKRLITVVVIILVYLAGAFIYGKASGEKESTSTNQTKKTTVV
                     GVLQYVSHPALDEIYRGIKDGLKEEGLKEGENLEIKFQNGQADQSKLATMSQQLVQSD
                     PDALIGIATPAAQSLANTTSSIPVVLGAVTDPVGAGLVASLEKPGGNVTGVSDQPPVT
                     SQIKLASDLLPNAKKVGILYASSEDNSKYQVAQAEEAAKKEGLESVKYAVPSTNEIAQ
                     TVQVMSRQVDFIYVPLDNTIANAMQTVVKEANKANIPVIPSVDTMVEQGGLATIGINQ
                     YQLGLQSGKMAAELASGKEKPETTPVYTFDQGDTVINQSQADRLGIIVPQSMKEKAKI
                     ITDDSQQETSKEDDK"
     gene            2142..3023
                     /locus_tag="ACG8KR_002144"
     CDS             2142..3023
                     /locus_tag="ACG8KR_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0201475.1"
                     /translation="MIVSAISQGMLWAILGLGIFMTYRILDFPDMTTEGSFPLGAAVC
                     VTAITHGVAPIVATLLGVGAGMIAGLVTGLLYTKGKIPVILAGILVMSGLNSVILFVM
                     RTPNLSLLNHPILQDSFHRFGLPDYFDTVLLGFISLGILIAVLLFFFNTDLGQGYIAT
                     GDNETMARSLGIKTDRMKILGLTLSNGVIALSGALIAQNDGYADVNKGIGVIVIGLAS
                     IIIGEVIFQELTLAERLIAIVVGSIIYQLLILVVIKLGFDTTYLKLFSAIILAICLMI
                     PQLKKALNLKTGFEKEA"
     gene            3026..3796
                     /locus_tag="ACG8KR_002145"
     CDS             3026..3796
                     /locus_tag="ACG8KR_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0201476.1"
                     /translation="MTDILTIKNGRKVVNNGMNEEKVLLDEINLTIREGDFITVLGGN
                     GAGKSTLFNTIAGTLQLSQGSISFKDQLITKDSEEKRARFLSRVFQDPKMGTAPRMTV
                     AENLLLAQKRGQKRKFRLRNLKNQKEDFFRLCQEVGNGLEQHLDTPAGELSGGQRQAL
                     SLLMATITAPELLLLDEHTAALDPKTAKALMRLTNQRINEQKLTCLMITHRMEDALKY
                     GNRLIVLQKGKIVQDISEKEKQHLTMGDLLQFFEEVVD"
     gene            4075..4929
                     /gene="aroE"
                     /locus_tag="ACG8KR_002146"
     CDS             4075..4929
                     /gene="aroE"
                     /locus_tag="ACG8KR_002146"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HFQ0201477.1"
                     /translation="MISGQTKLAGLFADPASHSLSPLMHNTAFEANNIDAVYLAFQVN
                     PMNLKQAVESIRTFDMLGVNLSMPNKTAVIPYLDEVSQEAQLIGAVNTIVHEKGRLIG
                     YNTDGAGFMQSVQEAGISIKKKKMTLLGAGGAAKAVTVQAALDGAEEIVVYKRNNQTF
                     AQVKETFEQLSYRTSCKIKVRDFADTHQLRKDLKESCLLVNATDIGMGKKINQSPLLD
                     ISMMHENLAVFDLIYAPRETRLLKEAKKVGAKGYNGLGMLIYQGAIAFELWTKEKMPI
                     HVIEKLFD"
     gene            5107..6270
                     /locus_tag="ACG8KR_002147"
     CDS             5107..6270
                     /locus_tag="ACG8KR_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFQ0201478.1"
                     /translation="MSRMDRYKDIHKKAKPLKRDTGSIFARRERKRTRMENEEDATRI
                     YEREQHSFEHLNQYEQQIYRDAPRAEEQQKNKKFFNRQKKNKPLKPRRKRSWKKIILS
                     ILLVLILYSGISFFIGKYVAEHDTSLEAATSETFHGQKATNGAHNILILGSDTRGEDA
                     GRADTIMVLQLDGPSHKPKLISFMRDSFVTIPGVGQNKINSAYAYGGADLVRQTLVEN
                     FGIDCQYYAKVDFKSFEKVIDALFMNGVKIDAEKDLNLDGVDIKKGVQKMDGHVLLQY
                     ARFRMDEQGDFGRVRRQQQVMNAIFSQLKNPLNLIRAPYAAGKALGYTSTDVTAWFLT
                     KNLFSIARGAGGIDRISVPVDGSWNYGQSDYAGSILEIDNQMNQSAITDFLNK"
     gene            6387..6797
                     /locus_tag="ACG8KR_002148"
     CDS             6387..6797
                     /locus_tag="ACG8KR_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFQ0201479.1"
                     /translation="MDIYLNFKNESREAIAFYEKVFQTEASGITTYGEMPGIEQMNLP
                     EEAKNLITNARLNIYDTLVMFADTPEGIGPELVVGNNVSLVIQTRNEEETKRLFESLA
                     EGGKIEMPLDAVPWAQLYGMVTDRFGINWQINQE"
     gene            6971..9199
                     /gene="topB"
                     /locus_tag="ACG8KR_002149"
     CDS             6971..9199
                     /gene="topB"
                     /locus_tag="ACG8KR_002149"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFQ0201480.1"
                     /translation="MKTVILAEKPSQAKAYAETFQQQKRHEGYYEISDPLFAGEVTIT
                     YGFGHLVDMVAPGAYEERWAKWSLENLPIFPETFRYEVPMDKRAQFSIVKRELQSADT
                     IIIATDGDREGEAIAWSIIRQANAFSKEKNYLRLWINSLEKEAIYEGFKNLQPGKNYF
                     PKYKEAQARQNADWLIGMNGSPLYSLLLQRKGIDGSFSLGRVQTPTLYMIYQLQEEIK
                     HFKKETYFEGEGLITTNKGQFSGKIVPKKAFKTQEELTKEIEKLGAHLGKQNGRILEV
                     TKKEKRLHSPRLFSLSSLQTKMNQLMKASAKATLDAAQGLYESKFLSYPRTDSFYITE
                     NEHQYLVKHLQNYKKFLGIEEVQTTEIKAKKRYVDAKKVQEHHAIIPTKTIPTPARFA
                     KLSKLQQAIYLQVLRTTVAMFAADYIYEETTVMTGVQQLKLKSIGKIPLQQGWQEILR
                     IKSKKKEAQTLPPIVENEEAEVELKSMEKETQPPKPYTEGTLITAMKTAGKTLDNDEA
                     QEILKEVEGIGTEATRANIIENLKQKHYIEVNKNEITVTPKGITLCKAVANEPLLTSA
                     EMTARWEGYLRKIGKQEGTPSVFLENIKKFILHLLAEVPNQIDKVDFSDEISGTRQIK
                     AMEKKNDQLGRCPKCKKGIVMLYPKIATCLNPECDFKLWPTVAKKKLTKTNLRDLLSK
                     GKTSKVVKGFTGKKGKFDAVLVLKEDMTIGFSFPWIENSGTKAVDGNKETSRKNNENE
                     VE"
     gene            9370..9654
                     /locus_tag="ACG8KR_002150"
     CDS             9370..9654
                     /locus_tag="ACG8KR_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0201481.1"
                     /translation="MGAITIVAVYQAFLKYPDYGTKLLSLTHNQTAYSPTLILSTLTA
                     GAKSATYFFHKTTSMLKRKKQRKQLLVVVAAMIIIYFLPVITQVFHQYFG"
     gene            9814..10050
                     /locus_tag="ACG8KR_002151"
     CDS             9814..10050
                     /locus_tag="ACG8KR_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFQ0201482.1"
                     /translation="MKKMKKYKIRKSGNSDVTTIPPEVKEFMGVQTGDAISYVFQSDG
                     SVRMIKAQEEPDIDSLVDSIMNQYEDALKDLVDL"
     gene            10047..10445
                     /locus_tag="ACG8KR_002152"
     CDS             10047..10445
                     /locus_tag="ACG8KR_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HFQ0201483.1"
                     /translation="MRYLSTKEIIKINAKVILRHSPGEMIGVKDANALDMAVKQPSQA
                     VFNQELYPEIYEKAAILAINLAKKHPFHNGNKRTALVAMLLFLQLNNCPVAFSRQEAV
                     DFIIMITTSSLDFDSLKSKITSYLRQTAIK"
BASE COUNT         3670 a         1770 c         2230 g         2870 t
ORIGIN      
        1 aggagaaaaa gtagaaaaga aggtgtttgt tatttgggaa gtagttaaat cgagagtaca
       61 agaaatgaat gaacatttct cagtgtcatt cgcagaaaat ccaagtaagt attttcgacg
      121 tcttgttttt ctgctcatct tatcattgct gttaacagtt gcaatgtttt tattgccgga
      181 aaaaatccga gaagagcaag taaatagtct cgtttattcg tccataccaa ccagcaaatt
      241 gaaacctttt gattacagca aagctgatca gaaaatcaaa caaagccggg cgatatcggt
      301 gatgttcagc aaaccgaacg ggaaaaccta tcaggacatg cttgatgttt ttaatgatcc
      361 tgagcttatg gaagaattga atcgaccgat cttttattat ccgatcgttt atgatgtaca
      421 tgaactggaa caaaaatata acatacgaac ggatgaagtg acatttattt tctttgatgg
      481 aggaaaagaa gcaaatcgga taactgcggg taagggagga ataactgatt ttgataagga
      541 gttgatccct gaactgaatc gacttccgtt agccaatatc aaacaattag aagaagaatt
      601 ggatagaacg ggagtaactt cccaaaattc cgagattgct aattgagaca ttggcgcgat
      661 gaaacggtgg gtctatatag cgctattttt aattatcaga acaagtgatg tttgatcttc
      721 aaaataacaa ataaaaaatt aaaaaatata gttgacgaaa ttcaaaaatt cgattagact
      781 taaatcaatt cttaaggaaa gggtgacctg taatgaactt atcaaaacgt atttcagcag
      841 aaaacaaaaa aaattggcgc agctggcgta gataagttgt attaatggtg agactttccc
      901 atagatacaa ctttttagag tgcatttcta aagcttgtat ctatgctaga catgaacatt
      961 gactgccgca tagattagaa tctatgcggt tttttgtgta aagacaatga ccgctttggc
     1021 cattgtcttt ttttgtagac tggatacttg cctgattata ataaataaaa agattgactt
     1081 tgcaggagga aaaaaatgaa aaacaaacga ttaataacag ttgtagtaat tattttagtt
     1141 tatttagcag gtgcatttat ttatggaaaa gcctcaggcg aaaaagaaag tacttcgacg
     1201 aaccagacaa aaaaaacaac agttgttggg gtattgcaat acgtgagcca tccggcatta
     1261 gatgagattt atcgaggaat caaagatggt ctgaaagaag aaggattaaa agaaggagaa
     1321 aatcttgaga tcaaatttca aaatggacaa gccgatcaga gcaagctagc cacaatgagt
     1381 caacagctag tacaatctga tccagatgcc ttgattggaa tcgcaacacc tgccgctcaa
     1441 tcattagcaa acacgacttc atcgatccca gttgtgttag gtgctgtaac ggatcctgtg
     1501 ggggctggat tagtcgcttc tttagagaaa cctggtggta atgtaacagg tgtttctgat
     1561 cagccgcctg ttacttcaca aatcaagctt gcttctgatt tgttgccaaa tgcaaaaaaa
     1621 gtcggaatct tgtatgcttc ttcagaggat aattcaaaat accaagtagc acaagcagaa
     1681 gaagctgcaa aaaaagaagg gttagaatca gtaaaatatg cggtgccttc cactaatgag
     1741 attgcacaaa cggttcaagt gatgagccga caagtcgatt ttatctatgt tccattagat
     1801 aataccattg ccaatgcgat gcaaacagtg gtaaaagaag caaacaaagc aaatatcccc
     1861 gtcattccat ctgtggatac aatggtggaa caaggtggac tagcaacaat cgggatcaat
     1921 caatatcaat tagggcttca aagcggtaag atggcggcag aacttgcgag tggaaaagaa
     1981 aaaccggaaa cgacgcctgt ttatacattt gatcaagggg atacagtgat caatcaatct
     2041 caagctgatc gcttaggaat cattgttccc caatcgatga aggaaaaagc caaaatcatt
     2101 acagatgact cgcagcaaga aacatctaag gaggatgaca aatgatcgta tcagctattt
     2161 cacaaggcat gctttgggca atattaggat tagggatttt catgacttat cggatcttag
     2221 actttcctga tatgacgaca gaagggtcct tcccattagg tgctgctgtc tgtgtgacag
     2281 ctattactca tggggtagcg ccgattgtag ctacattatt aggcgtaggt gctggcatga
     2341 tagcaggact cgttacgggg ttactttata caaaaggaaa gataccggtt attttagcag
     2401 ggattttagt gatgtcagga ttgaactcag ttattttgtt tgtgatgcga acaccaaatc
     2461 tttctttatt gaatcatccg attctacaag acagcttcca tcgctttggg ttacctgatt
     2521 actttgacac agtccttctt ggcttcatct ctttgggcat cttgattgct gtactgctat
     2581 tcttcttcaa tacagatttg ggtcaaggct atattgcgac aggagacaac gaaacgatgg
     2641 ctcggtcgtt agggataaaa accgatcgta tgaaaatttt aggtttaact ttgtcgaacg
     2701 gtgtcattgc attatccggt gcgctgattg cccagaacga cggatatgca gatgtaaata
     2761 aagggattgg cgtaatcgtc atcggtcttg cttccatcat catcggtgaa gtgatcttcc
     2821 aagaattgac gctagcagaa agattgattg ctattgttgt cggcagtatc atttatcagc
     2881 tattgatttt agtagtaatc aaactagggt ttgacacgac ttatctgaag ttgttctccg
     2941 ctatcatctt agctatttgt ctgatgatcc cgcaattgaa gaaagcactg aacttaaaaa
     3001 cgggttttga gaaggaggca tagtaatgac tgatatattg acaatcaaaa atggcagaaa
     3061 agtcgtaaac aatggaatga atgaagagaa agtactattg gatgaaatta accttacaat
     3121 cagagaaggg gacttcatca ctgtccttgg cgggaatggt gctggaaaaa gcacactgtt
     3181 caatacgatc gctggtacgc ttcaactcag tcaaggatcg atctctttta aagatcagct
     3241 gatcacaaaa gattcagaag aaaaaagagc acgattcctt tctcgagttt tccaagaccc
     3301 caagatggga acggctcctc ggatgacagt agcagaaaat ttgctacttg cacaaaaaag
     3361 aggtcaaaaa cggaaattcc gtttacgtaa cttgaaaaat caaaaagaag attttttcag
     3421 actatgtcaa gaagtcggta atggcctaga gcaacacttg gatactccag cgggtgaatt
     3481 atctggtggt caacgccaag cattaagctt gctgatggcg acgatcacag ctcctgagtt
     3541 actattatta gatgaacata ctgcagcttt agatcctaaa acagctaaag cattaatgcg
     3601 attaacaaat cagcggatca atgaacaaaa attgacttgt ctcatgatca cgcatcgaat
     3661 ggaagacgcc ttgaaatacg gaaatcgtct gatcgtactc caaaaaggga aaatcgttca
     3721 agatatttct gaaaaagaaa agcaacattt gacaatgggc gaccttttgc agttctttga
     3781 agaagtagtg gattgatttc gacagaaaaa ttcctttcag tagaaggact tgaaaactca
     3841 ttgacatctt cctttgattc atcctatact taaaacaata aacaattaaa catatttggg
     3901 tgttttgtag gatcacgtag tcaatgattt taacaattaa atagaatatt ctttgctctg
     3961 acaagtaaac gatgagaaaa gaatttgcta ctttgccacc tagacgtttc gtactttttc
     4021 gtatcaaacg ctaagtggct ttttttattg aagtaagaac aaaggaggaa taagatgatt
     4081 tctggacaaa ccaaattagc tgggctcttt gctgatccgg cttctcacag tttatcccca
     4141 ttgatgcaca atactgcttt tgaagcaaac aacatcgatg cggtgtattt agctttccaa
     4201 gtaaatccaa tgaatttgaa gcaagcagtc gaaagtattc gaacttttga tatgttaggt
     4261 gtaaatcttt cgatgccgaa taaaacagcg gttattcctt atctggatga ggtaagccag
     4321 gaagcgcaat tgatcggagc agtgaatacg atcgtgcacg aaaagggtcg tttgattggc
     4381 tataataccg atggtgcggg cttcatgcaa tccgtgcaag aagctgggat ttcgatcaaa
     4441 aagaaaaaaa tgactctact gggagctgga ggtgcagcaa aggcagtcac tgttcaagct
     4501 gcgttagacg gcgctgagga aatcgtagtt tataaacgaa acaaccaaac attcgctcaa
     4561 gtaaaagaaa cattcgaaca actcagctat cgaacttctt gtaagataaa agttcgtgac
     4621 tttgccgata cgcatcaatt aagaaaagac ttgaaagaaa gctgtcttct agtcaatgca
     4681 acagatatcg ggatgggaaa gaaaatcaat cagtcaccgc tcttagatat aagcatgatg
     4741 catgagaatc tagcggtttt tgatttgatt tatgcaccta gagaaacacg tttattaaaa
     4801 gaagcaaaaa aagtaggagc aaaaggatac aatggacttg gcatgttgat ctaccaagga
     4861 gcaatcgcgt ttgaattatg gacaaaggaa aagatgccga tccatgtgat tgaaaagctg
     4921 tttgattaaa aaaggataat agataaagat cttttattct cgcataaacg gtctcaagaa
     4981 aagtaatcct ttatgtatcg aagcatttat ttcgttattt tttattaaat tatcaaaaaa
     5041 tgataggctt ttgtcgattg gttatcgtat cctttattta tcagtaagaa acgaggagga
     5101 accacaatga gtcggatgga tcgctataag gacatccata agaaggcgaa acctctgaag
     5161 cgggatacgg ggagtatttt tgctagaaga gaacgaaaac gaacgcgtat ggaaaatgag
     5221 gaagatgcta cccgtattta tgaaagagag cagcattcat ttgaacattt gaaccaatac
     5281 gagcaacaga tatacagaga tgcaccaaga gcagaagaac aacaaaaaaa taaaaaattt
     5341 ttcaatcggc aaaagaagaa caaaccgtta aaacctcgaa gaaagcgatc gtggaaaaaa
     5401 atcattctat cgattttgct tgttttgatc ctttattcag gtatctcatt ttttattggt
     5461 aaatatgtag ctgaacatga cacaagctta gaggcagcga cttcagaaac ctttcatggg
     5521 caaaaagcga cgaatggtgc gcataatatt ttgatccttg gaagtgatac acgcggagaa
     5581 gatgccgggc gagccgatac gatcatggtc ttacagttgg atggtccttc ccataagccg
     5641 aaactgattt cttttatgcg tgacagcttc gttacgatcc caggggtagg acaaaataag
     5701 atcaattcag cttatgcata cggaggagcc gatctagtgc gtcaaacttt agtagaaaat
     5761 tttggaatcg attgtcaata ctatgcgaaa gtcgatttca aatcgtttga aaaagtgatc
     5821 gatgccttat tcatgaacgg agtgaagatc gatgcagaaa aagatctgaa tctggatggt
     5881 gtagatatta aaaaaggggt ccagaagatg gatggacatg ttcttttgca gtacgcacgt
     5941 ttccgaatgg atgaacaagg agactttggc cgtgtcagac gccaacagca agtaatgaac
     6001 gctattttca gccaattgaa gaatccattg aatctgatcc gtgcacctta tgcagctggt
     6061 aaagctttag gatatacatc cacagatgtc actgcttggt tcttgacgaa aaacctcttt
     6121 tcgattgcac gaggagctgg tggcattgat cgaataagtg tgccagtaga cggttcgtgg
     6181 aattatgggc aaagtgatta tgctggcagt attcttgaaa ttgataatca aatgaatcaa
     6241 tctgctatta cagatttttt gaataaatga tcataaaaaa gatcaagaag aagctgcgca
     6301 aggcttttgt catgaatcaa tcaaacgatt cactcgtaaa aaacgctttc ttatggtaga
     6361 tttaaccaag agaggagctg tttacattgg atatctatct gaacttcaaa aatgagtcgc
     6421 gtgaagccat cgctttttat gaaaaagttt ttcagactga agcatcaggt atcaccacat
     6481 acggtgaaat gcctggaata gaacaaatga acttgccaga agaagcaaaa aatctaatca
     6541 cgaatgcacg gctgaacatt tatgatacat tagttatgtt tgcagatacg ccagaaggaa
     6601 ttggtcctga attagttgtg ggaaataatg tttctttagt catccagaca agaaatgaag
     6661 aagaaacgaa gcgcctattt gaaagcttag ctgagggtgg taagattgag atgccactgg
     6721 atgctgttcc gtgggctcag ttgtatggga tggtaactga tcgatttggc atcaactggc
     6781 agattaacca agagtgacag ctaaagatga atattttctg gaaacatcaa gcagcaaacg
     6841 gatcatcgtt tgtttgcttg atgtttttgt gtctatttcg actatacggt atcttttttt
     6901 tattgccaga tgcgaaaata tgttccataa tagagaagtc cgcaaaaaat taagtaacgg
     6961 aggtcagaag atgaagacgg tgatactagc tgaaaaacct tcacaagcaa aagcctatgc
     7021 cgaaaccttc caacagcaaa agcggcatga agggtattat gaaatcagcg atccattgtt
     7081 tgctggtgaa gtaaccatca cttatggttt tggacatttg gtagatatgg ttgctccagg
     7141 agcttatgaa gagcgttggg caaaatggtc tttagaaaat ttgcctattt tcccagaaac
     7201 cttccgttac gaagttccaa tggacaaacg tgcacaattt tcgatcgtaa aacgagaatt
     7261 gcagtcagcc gataccatca ttattgcaac agatggtgat cgagaaggtg aagccatcgc
     7321 gtggtcgatt attcgacaag caaacgcatt ttctaaggaa aagaactatt tacgtttatg
     7381 gatcaattct cttgaaaaag aagcaatcta tgaaggtttt aagaatctcc aacctggaaa
     7441 gaactatttt ccaaaatata aagaagcgca agcaaggcag aacgctgatt ggctgattgg
     7501 aatgaatgga agtccattat atagcttgct acttcaacga aaagggattg atggcagttt
     7561 ttctcttggt cgtgtacaaa caccgacact ctatatgatt tatcagctgc aagaagaaat
     7621 caaacacttc aaaaaagaaa cgtactttga aggagaaggt ttaattacga caaacaaagg
     7681 gcagttttct ggaaaaatcg taccaaaaaa agcatttaaa acccaagaag agttaacgaa
     7741 ggaaatagaa aaacttggag cgcatttagg taagcaaaac ggacggattc ttgaagttac
     7801 gaaaaaggaa aagaggttgc atagtccacg tttattttcg ctatctagtt tgcagacaaa
     7861 gatgaatcag ctaatgaaag caagtgccaa agctacactt gatgctgctc aaggattgta
     7921 cgaaagcaag tttctaagct atccaagaac ggattccttt tacattacag aaaatgagca
     7981 tcagtatctg gttaaacatc tgcaaaatta taaaaaattt ttaggaatag aagaagtaca
     8041 gacaactgag atcaaagcaa agaaacgata tgtcgatgcg aaaaaagttc aagaacatca
     8101 tgcaattatt ccgacaaaga cgattccgac tcctgcacgc tttgcgaagc tttccaaatt
     8161 acagcaagca atctatttgc aagttttgcg cactactgtg gctatgtttg ctgcagatta
     8221 tatctacgaa gagacgaccg taatgacggg cgtacagcaa ttaaagctaa aaagcatcgg
     8281 aaagatccca ttacagcaag gttggcagga aatcttacgc atcaagtcaa aaaagaaaga
     8341 agctcagact cttcctccta tcgtagaaaa tgaagaagca gaagtagaac tgaaaagtat
     8401 ggagaaagaa acacaacctc caaaacccta taccgaagga acgttgatca cagcaatgaa
     8461 aactgcggga aaaacgctgg ataatgatga agcgcaagaa attctcaaag aagtagaagg
     8521 aatagggaca gaagctacac gagccaatat cattgaaaat cttaagcaga agcattacat
     8581 cgaagtcaat aaaaacgaaa taactgttac gccaaaagga atcactcttt gtaaggcagt
     8641 agccaatgaa ccattactga cgagcgcaga aatgactgcg cgctgggaag ggtacttgag
     8701 aaaaattggt aaacaagagg ggacaccatc tgttttttta gagaatatca aaaaattcat
     8761 cctacattta ttggcagaag ttcctaatca aatcgacaag gtagactttt cagatgagat
     8821 tagcgggaca cgtcagataa aagcgatgga gaagaaaaac gatcaattag gaagatgtcc
     8881 aaaatgtaaa aagggaatag tcatgcttta tcctaaaatc gctacatgct tgaatccgga
     8941 atgcgacttc aaattgtggc caacagtagc taaaaagaag ctgactaaaa cgaatctcag
     9001 agatttgctt tcaaaaggaa agacaagtaa agttgtcaaa ggttttactg gtaaaaaagg
     9061 gaaattcgat gcagttttag tattaaaaga agatatgaca atcggttttt cttttccatg
     9121 gatcgaaaac agtggaacaa aagcagttga tggaaacaaa gaaacatcaa gaaaaaataa
     9181 tgaaaacgaa gtagagtaaa tgaaaacaat gaaatagcgg aatatttatt ccgaaaagtg
     9241 agaatataaa taccaataaa actaaaaaat atcagtgaat caagcaaaaa cgttggggta
     9301 ggctgatttt atatagatag caaggagttt ttttatttga aaaaagtgca ttcattttta
     9361 aattatctta tgggagcaat cacgattgtg gctgtgtacc aagcatttct aaagtatcct
     9421 gattacggga cgaagttgtt gtcattgacg cacaatcaaa cagcatattc accaacgttg
     9481 attttatcga ctttgactgc aggagctaaa agcgctactt atttttttca taaaacgaca
     9541 agcatgctaa agcgaaagaa acagcgaaaa caattgttgg tggtggtagc ggctatgatc
     9601 attatttatt ttctaccagt tattacacaa gtatttcatc aatattttgg ttaagaatac
     9661 gagagtttgt gacaaaagtc atgtcacaaa cttttttctt tgtcattttt tcgtaagaaa
     9721 aagcaaaata aaagtatata cattgattaa aagtatatac ttggatataa tgtacttact
     9781 atagaatgag aatggtatag agggtggcga aatatgaaaa aaatgaaaaa gtataaaatt
     9841 agaaaatctg gcaatagcga tgtaacaact atcccaccag aagtaaaaga attcatgggt
     9901 gtgcagacag gtgacgcaat cagttatgtt tttcaatctg atggttctgt acgaatgatc
     9961 aaagcacaag aagaacctga tattgattca ttagttgatt cgatcatgaa tcaatatgaa
    10021 gacgcgttaa aagatttggt tgatttatga ggtatctttc gactaaagaa attatcaaaa
    10081 tcaacgcgaa agttatttta cgtcactcgc caggcgaaat gatcggtgta aaagatgcaa
    10141 atgcattaga tatggccgtc aaacagcctt cacaagcagt attcaatcaa gaactctatc
    10201 ctgaaatcta tgaaaaagcg gcgattttag caattaattt agctaaaaaa catccttttc
    10261 ataatggcaa taaacgaact gctcttgttg ccatgttgtt gtttcttcaa ttgaataatt
    10321 gtccagttgc tttttctcgt caagaagctg ttgattttat cataatgatt accactagtt
    10381 cattggattt cgattcacta aagtccaaga taaccagtta tttacgacaa accgcaataa
    10441 aatgaacgaa ttaaaaactt tatttctcaa ttagatcagc atgtcattaa ggaatatgct
    10501 gattttttta tgtttaatca tacaaaatag ttttttcata
//
LOCUS       DAZKLD010000103        10349 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.103, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000103 DAZKLD010000000
VERSION     DAZKLD010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10349
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.103"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..853)
                     /locus_tag="ACG8KR_002153"
     CDS             complement(<1..853)
                     /locus_tag="ACG8KR_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HFQ0201484.1"
                     /translation="MIKYFTLDNEKLRNTGKETDQTIWYCVERPNQEEIHQLTHQFEI
                     PSDYITSILDDAENSRTEEFNQSKLTKPALLLLQYPFAKTSPSGYFQVDTYPLSIILT
                     PKKKLITITNHEPLFLKKVFSQTFPKSDISPNLNIFFQFIWQVTLSYNSYLATLQSQC
                     DTLEAKLQVSTENKQLYQIMDIQKSLVYFREATSANLDALKELAQNKLFQENHALKNH
                     LRDVLVETEQAMTTSRIQLKLVEQMNQTFSAIVSNNLNNIMKILTSLTIILTIPTIVG
                     SIYGMNIK"
     gene            1024..1392
                     /locus_tag="ACG8KR_002154"
     CDS             1024..1392
                     /locus_tag="ACG8KR_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HFQ0201485.1"
                     /translation="MGTFTPLILFWYIYPVIVLFGCQFLVKLFSLNRRFKIKAPDLAI
                     PFLWGGMHALSRNTGTISILPFFFISVLLMGICIAVFQAYYYEEIIYPRYFKMTWRLV
                     FLLTIVLYAVLIIVNILSYL"
     gene            1562..1993
                     /gene="mraZ"
                     /locus_tag="ACG8KR_002155"
     CDS             1562..1993
                     /gene="mraZ"
                     /locus_tag="ACG8KR_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HFQ0201486.1"
                     /translation="MLMGEFQHNIDAKGRLIVPSKLREELGEKFVLTRGLDGCLFGYP
                     MSEWENLEAKLNEMPLAKKDARTFVRFFYSAATECELDKQGRINIPSTLRNYAALTKG
                     CVVIGVSNRIEIWDETRWQDFSAAAEENFDEIAESMIDFGL"
     gene            2014..2973
                     /gene="rsmH"
                     /locus_tag="ACG8KR_002156"
     CDS             2014..2973
                     /gene="rsmH"
                     /locus_tag="ACG8KR_002156"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HFQ0201487.1"
                     /translation="MTETFQHYTVMLKETVDGLNIKPDGVYVDCTLGGAGHSEYLLSQ
                     LSSKGHLYAFDQDQKAIDHAKLRLASYVEQGMVTFIKANFRELEERLSEHVDKVDGIL
                     YDLGVSSPQLDEAERGFSYHQDAPLDMRMDQSAPLSAYHVINEYSYHELVKIFFRYGE
                     EKFSKQIAREIERVRKTKPIQTTGELVEIIKQVIPAPARRKGGHPAKRIFQAVRIAVN
                     DELGAEEASLEQAIRLLKVNGRISVITFHSLEDRIVKSMFKEYSTVQDLPPGLPVIPD
                     EFQPELKVITRKPILPSEEELTENNRSRSAKLRIAEKIRMKEE"
     gene            2978..3379
                     /gene="ftsL"
                     /locus_tag="ACG8KR_002157"
     CDS             2978..3379
                     /gene="ftsL"
                     /locus_tag="ACG8KR_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HFQ0201488.1"
                     /translation="MAELKKMQDYEYDLPELVDQPEQQMPDSSSNQEIILPQSPAKRL
                     KQISALEKIAVASVAIAIVALCVLTVMLRTNISGAEKEITSIQTEINDKKQEKTSLEQ
                     EKNELSRTERIKQIAEEKGLSINDDNLRKVK"
     gene            3380..5572
                     /locus_tag="ACG8KR_002158"
     CDS             3380..5572
                     /locus_tag="ACG8KR_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HFQ0201489.1"
                     /translation="MSLKNKIRRFMEKKNLNPMNNRKKVGIILFATSIGMFFLFAVRF
                     SYIVIGGHVAGTSLAEKTKQLYQGSEVVKAKRGTIYDRNGVALAEDASSYSIKAILSK
                     TYTSGDKKLYVEEKNFDKIAEILHKNLSIDKKDALNILEDGAKKELYQVEFGSYGKNI
                     SQETKQNIEADMKKEGVAGLYFVDHQARMYPNGVFSSHFIGYAVPDKDENGLVGKLGL
                     ESAYNDILSGKDGKIIYQKDNFQNPLPGTVAEEEKAVDGQDIYTTLDSRLQSYLETLM
                     DQVNEEYQPEELTAVLMKAKTGEILAMGQRPTFNPETMEGLTGKDAIWRNFLVQDSYE
                     PGSTMKVFTTAAAIEEGEFNENETFQSGKIQVEDATINDHDFGEKGVLTMRQALSWSS
                     NVGMVILEQRLGGRWYNYLQKLGFGQSTHSGLDDEVNGALPTLNIVDRAMSAYGQAVG
                     VTNFQMMKAFTSIANNGTMIQPRYISKVVDPQTGEERTTQTEVLGQPFSKETTEKVRE
                     YMRDVVESENYGSAYGVYSVPGYNVSAKTGTAQIASDTGGYQTGDTAYLYSIVEMVPS
                     EDPDYVLYLTMKHPKTYDRMALAKIANPLMKRAMDFKETEEDSDTETKTEKVSVADYR
                     NLEADVAAADAQKSGLQPVVIGNGKKVQKQSTANGDQLISGEKLILYTGGDKLMPDVT
                     GWSKADIMKLGKILGIEVSFDGDGYCVKQELAPYEKITEDKLSFTLEE"
     gene            5598..6560
                     /gene="mraY"
                     /locus_tag="ACG8KR_002159"
     CDS             5598..6560
                     /gene="mraY"
                     /locus_tag="ACG8KR_002159"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HFQ0201490.1"
                     /translation="MEWTQALIPIVSSCAMTIAAMPLFIGYFQMKKQGQAIREEGPKW
                     HNSKAGTPTMGGLVFLIGSILTGIWVGAWQKQLTPTLFILLFVLALYGVIGFLDDFIK
                     IFKKRNMGLNSKQKLIGQILGGIIFYLVYRSEGYPGVLNFFGLDLPLGIIYGIFALFW
                     LVGFSNAVNLTDGIDGLVAGLGSISFAAYGLIAWHQHQYDVLIICLSVLGGLLGFFVY
                     NRKPAKIFMGDVGSLALGGLLAAISIMLNQEWTLLLIGLIYVMETASVMLQVTSFKLT
                     GKRIFKMSPIHHHFEMCGWSEWKIDTVFWLISIVTSLITLWIVW"
     gene            6574..7935
                     /gene="murD"
                     /locus_tag="ACG8KR_002160"
     CDS             6574..7935
                     /gene="murD"
                     /locus_tag="ACG8KR_002160"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HFQ0201491.1"
                     /translation="MKKISTYENKKVLVLGLAKSGVSAAKLLHELGALVTVNDGKPFD
                     ENPEAQELLSLGIKVITGSHPIELLDEEFSLMVKNPGIPYSHPLVAKAQEMGIPVITE
                     VELAYEVAECPIIGITGTNGKTTTTTMTGLLLNAGADQGIARLAGNIGYPASGVAQEA
                     KSEDKIVMELSSFQLMGITDFRPHIAVITNIYEAHIDYHGTRKEYVKAKWNLQKNMTE
                     KDYLILNWNQSELQELAQRTKARVLPFSTKEVLEDGVYADDYSIYYKKEKIMEISELG
                     VPGKHNVENALAAISVAKLYGISNEAIRETLHFFHGVPHRTQYVGEIQGRKFYNDSKA
                     TNILATKMALSGFETSKVVLLAGGLDRGNTFDELIPSLKGIKAMVVFGQTKEKLMDAG
                     KKAGIETIVTADSVEQAVPLALENSTDGDVVLLSPANASWDQYPNFETRGNRFMEAVN
                     RLK"
     gene            7961..9049
                     /gene="murG"
                     /locus_tag="ACG8KR_002161"
     CDS             7961..9049
                     /gene="murG"
                     /locus_tag="ACG8KR_002161"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HFQ0201492.1"
                     /translation="MKILVTGGGTGGHIYPALAFVNYVKTQEPNAEFMYVGAKRGLEN
                     KIVPDTGMPFHTLEIQGFKRKISMHNIKTVRLFLKSIRQAKKILSDFQPDIVIGTGGY
                     VSGAVVYAAAKMGIPTVIHEQNSVPGVTNKFLSRYVDRIALSFKDAASFFPENKAVLV
                     GNPRAQEVADTKKSDILKTFDLDPEKKTVLIFGGSQGALKINQAVSVFLSEYIPSSYQ
                     VLYASGDRYYEEILNKINPEIQNISVQPYIKNMAEVMASSDLLVGRAGATSIAEFTAL
                     GLPAVLVPSPYVTNDHQTKNAMSLVHAGAAKMIADNELTGESLSQTVNEIMGDEELQK
                     QMCRASKEQGIPDASKRLYDLVKQIIKK"
     gene            9064..10284
                     /locus_tag="ACG8KR_002162"
     CDS             9064..10284
                     /locus_tag="ACG8KR_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HFQ0201493.1"
                     /translation="MSKKKEPKKLSHPSSDENNLTPWQKANRKYLAEQEMKNQEPKKE
                     DITLDTPLSDSEEEKTVQEEQQEESQSEKKIEFFEEIKQKKNGGPYNGSFLNRLPNLK
                     SQRHKVLFRRLALIITVLGIPLIFLIYYVSPYSKLQAVAVSGNKTVNSQEIISDTKLS
                     LGENVWGQYFHRSTYIDRLKKAQPRIETANIHFKGMNEFDLDVTEYKEIALIAKNNQY
                     YPVIENGTVLDEKVANPTKNLPILEEFKDSAKIKELTKQYNQLSSELQKAISEIKYTP
                     RASNKNLIQLNMNDGNQVIVNINNLANQMKYYSQVAKDMDEKGVIDMEVGIFSHPYGS
                     TQETEESGQSEETTESEENADQTNESSEEAETQKAQTPGSSSTNGSSDSNDSSETSQI
                     EDNPSTTPSSLNEN"
BASE COUNT         3583 a         1717 c         2188 g         2861 t
ORIGIN      
        1 acttaatgtt cattccatag atactgccga cgattgtcgg tatagttaaa ataatcgtta
       61 aagaagttaa aattttcata atattattca gattattcga tacgattgct gaaaaagtct
      121 gattcatttg ttctactagt tttaattgaa tgcgagaagt agtcattgcc tgctctgttt
      181 ccaccaatac atcacgtaaa tgatttttca aagcatgatt ttcttggaaa agtttatttt
      241 gcgctagttc tttcaatgca tctaaattag cagaagtcgc ttctctgaaa taaacgaggc
      301 ttttttggat atccatgatt tgataaagtt gtttgttttc agtggatact tgaagtttgg
      361 cttccaaagt atcgcattga gattgaagcg tagctaaata actgttgtaa gagagtgtga
      421 cctgccaaat gaattgaaag aagatattga gattaggcga aatatcactt tttggaaagg
      481 tctggctaaa gactttcttt aaaaacagtg gttcatgatt cgtaattgtg ataagcttct
      541 ttttgggcgt caaaataata gagagcgggt atgtatccac ttgaaaataa ccgctaggac
      601 ttgttttagc aaaaggatat tgtaacaata ataacgctgg ctttgtcaac tttgattgat
      661 tgaattcttc tgtacgggaa ttttctgcat cgtccaatat ggaagtgatg taatctgacg
      721 gaatttcaaa ttgatgtgtc agctgatgaa tctcttcttg attaggacgt tctacacaat
      781 accagatcgt ttggtcagtt tcctttccgg tattacgcag tttctcatta tctaatgtaa
      841 aatatttgat catgattttc ctcctgttgc ctgtttcttt tattataacg gatagggaat
      901 tggaatacat ggaatgtgtt tgtaagatat agaatataac tgcataaatt taaataaaat
      961 gaaacagttg tgtaaagtat atacagtata cttgaagcaa cgataaaaaa ggagtttacg
     1021 ttcatgggca cattcacacc acttatcttg ttttggtata tttacccggt aatcgttttg
     1081 tttggttgcc aatttcttgt aaaattattt tcgttaaatc gtcgttttaa aataaaagcc
     1141 cctgatttgg cgattccttt tttgtggggc ggtatgcatg cattgtctag aaatacaggg
     1201 acaatttcta tcttaccgtt tttctttatt tctgttttgc taatgggaat ttgtattgct
     1261 gttttccaag catattatta tgaagaaatt atttatccgc gttatttcaa gatgacttgg
     1321 cgtttggttt ttctgttgac aatcgtcttg tacgctgtct tgatcattgt gaacatactt
     1381 tcttacctat gaaaaaaata gaaaagctgt aaaaagtctt ttaaaaggct ttttgcagct
     1441 tgatttttaa aaaaatgttt atgaataaaa aaagttgttc ccggatttcc cagaaaaggt
     1501 ggtagaaagt gggggattgt ggtagactaa ttttagttag tggaggaatg gggggctttc
     1561 aatgttgatg ggtgaattcc aacataatat tgatgcaaaa ggacgtttaa tcgtgccctc
     1621 aaaacttcgt gaagaacttg gcgaaaagtt tgttctgaca cgcggattgg atggctgtct
     1681 tttcggctat ccgatgagcg aatgggaaaa tctggaagct aaactgaacg aaatgccgtt
     1741 ggctaaaaaa gacgcacgta cttttgtgcg attcttctat tcggctgcca ctgaatgcga
     1801 acttgataaa caaggacgaa tcaatatacc cagcacgtta agaaattatg cagctttgac
     1861 aaaaggttgc gtagtcatcg gtgtatctaa ccgaattgaa atttgggatg agacacgatg
     1921 gcaagatttt tctgcagcag cagaagagaa ctttgacgaa attgctgaaa gtatgattga
     1981 tttcggacta taagaatagg aagaggctga ctaatgactg aaacatttca acactataca
     2041 gtcatgttga aagaaacggt agatggattg aacatcaaac cggatggcgt ttatgtagac
     2101 tgtacactcg gcggtgccgg acatagtgaa tatttattaa gccaactaag ttctaaaggg
     2161 catctgtatg cgtttgacca agatcaaaaa gcaatcgatc acgccaagct gcgtttagct
     2221 tcttatgtcg aacaaggtat ggtcacattt atcaaagcga acttcagaga attagaagaa
     2281 cgcttgagtg agcatgtaga taaggtagac ggcattttgt atgatctagg ggtttcctct
     2341 cctcagctag atgaagcaga acgtggattt agttatcatc aagatgcacc gttggatatg
     2401 cgcatggatc agtcagctcc tttatccgct tatcacgtaa ttaatgaata ttcctaccat
     2461 gaattagtaa agattttctt ccgctatggc gaggagaaat tttccaaaca aattgctcgt
     2521 gaaatcgaac gagtacgaaa aacaaaacca atccaaacaa ctggtgagct tgtcgaaatc
     2581 attaaacaag tcatcccagc tcccgcaaga agaaaaggcg ggcatcctgc aaaaagaatt
     2641 tttcaagctg tgcggattgc agtaaatgac gaactcggtg ccgaagaggc ctctttagaa
     2701 caggcaatcc ggttattgaa agtcaatgga agaatcagcg taatcacatt ccattcattg
     2761 gaagacagaa ttgtcaaaag tatgtttaaa gaatatagta cggtacaaga tctaccaccg
     2821 ggacttcctg tcatacctga cgaattccaa ccagaactga aagtcattac tcgtaaacca
     2881 atccttcctt cagaggaaga gctaacagaa aacaatcgat cacgcagtgc aaagttacgt
     2941 attgcagaaa aaataagaat gaaagaggag tagaatgatg gcagaattaa agaaaatgca
     3001 agattacgaa tatgatttac ctgaattggt ggatcagccg gaacaacaaa tgcctgattc
     3061 ttcttcgaat caagaaatca ttcttccaca atcaccggca aaacgattaa agcagatttc
     3121 tgctttggaa aaaattgctg tggcaagcgt ggcgattgct attgttgctt tatgtgtttt
     3181 aacagttatg ctgcgcacaa atatcagcgg agctgaaaaa gaaatcacgt caatacaaac
     3241 agaaatcaat gataaaaagc aagaaaaaac gagtttagaa caagaaaaaa acgaactttc
     3301 tcgtactgaa cggattaaac aaatcgcaga agaaaaagga ttgtctatta atgacgacaa
     3361 tttaaggaaa gtgaagtaaa tgagtcttaa aaataaaatt cggcggttta tggaaaagaa
     3421 aaatctaaat ccgatgaaca atcgcaaaaa agtaggtatc attttatttg ctacgagtat
     3481 tggaatgttc tttttatttg ccgtccgttt ctcgtatatc gttattggag gtcatgttgc
     3541 tggtacctca ttagctgaaa aaacaaaaca gttgtaccaa ggaagtgaag tagtaaaagc
     3601 aaaaagagga acgatttatg atcgtaacgg tgtggcgctc gcagaagatg cctcctctta
     3661 ttcaattaaa gcgatactct caaaaacata tacctctggg gataaaaaac tgtatgttga
     3721 agaaaagaat tttgataaaa tagcagaaat cctgcataag aatctttcta tagacaaaaa
     3781 agatgcactg aatattttag aggacggcgc aaaaaaagag ctgtatcaag ttgaatttgg
     3841 tagctatggg aaaaatatct cccaagaaac caagcaaaac atcgaagcgg acatgaaaaa
     3901 agaaggtgtc gctggtcttt attttgtcga ccatcaggct cggatgtatc caaatggcgt
     3961 cttttcttct cattttattg gatacgcagt tccagacaaa gatgagaatg gcttagttgg
     4021 taaactaggt ttggagtctg cttacaatga tatcttgagc ggtaaagatg gtaagattat
     4081 ttatcaaaaa gataattttc aaaatcctct gccaggtaca gtggcagaag aagaaaaagc
     4141 tgtcgacgga caagatatct acacgacttt agacagcaga ctacaaagtt atttagaaac
     4201 actgatggat caagtaaatg aagaatatca gccagaagaa ttgacagcag tcctgatgaa
     4261 agcaaaaacc ggagaaatct tggcaatggg acaacgacct actttcaatc ctgaaacaat
     4321 ggaaggattg actggaaaag atgctatctg gcgtaatttc ttggtacaag atagttatga
     4381 accaggatca accatgaaag ttttcactac tgcagctgct atcgaagaag gagaattcaa
     4441 cgagaatgaa accttccaat ctggaaaaat ccaagtagaa gatgctacga tcaatgacca
     4501 tgactttggt gaaaaaggtg tgctgaccat gcgtcaagct ctttcatggt caagtaacgt
     4561 cgggatggtc atactggaac agcgattagg cggacgatgg tataactatt tacaaaaact
     4621 gggatttgga caaagtactc attctggatt agatgatgaa gtaaacggtg ctttacctac
     4681 attaaatatt gtcgaccgag caatgagtgc ttatggacaa gcggtaggtg tgacgaattt
     4741 ccaaatgatg aaagctttca cttctattgc gaataacggt acaatgattc aacctcgata
     4801 tatcagcaaa gtggtggatc cccaaactgg agaagagcga acaactcaaa cagaagttct
     4861 agggcaacct ttttcaaaag aaacaacaga aaaagtccgt gaatatatga gagatgttgt
     4921 cgaaagcgaa aactacggta gtgcttatgg tgtatatagt gtacctggat acaatgtatc
     4981 ggcaaaaacc ggaacagccc aaatcgcttc tgatacaggg ggatatcaaa caggagatac
     5041 agcctacctg tattcgattg tggagatggt tccttctgaa gatccagact atgtcttgta
     5101 tctaacaatg aaacacccta agacatatga tcgaatggca ttggctaaaa tcgcaaatcc
     5161 attgatgaaa cgggcaatgg attttaaaga aactgaagaa gattcagata cagaaacaaa
     5221 aacagaaaaa gtatccgtag ccgattatcg taatcttgaa gcggacgttg cagcagctga
     5281 tgctcaaaaa agtggactgc aacctgtagt gatcggtaat ggtaaaaaag tacaaaaaca
     5341 atctactgcc aacggtgatc agttgatctc tggagaaaaa ctgatcctat atactggtgg
     5401 tgataagctg atgcctgacg taacaggatg gtcaaaagca gatatcatga agctggggaa
     5461 aatcctcggt attgaagtta gttttgatgg agatggttat tgtgtcaaac aagaacttgc
     5521 tccatatgaa aaaatcactg aagacaaact tagttttact ttagaagaat aagaagagaa
     5581 ataggagaga aaagagcatg gaatggacac aagcattgat tcccattgtc agcagttgtg
     5641 caatgacaat tgccgcaatg ccgctattta ttggttattt tcaaatgaaa aagcaaggtc
     5701 aggcgattcg tgaggaggga ccaaagtggc acaatagcaa agccggaact cctacaatgg
     5761 gcggcttagt gtttctgatc ggctctattt tgacaggaat ttgggttgga gcttggcaaa
     5821 aacagctaac tcctactctt ttcatcttgc tttttgtatt agcgttatac ggagtaatcg
     5881 gctttcttga tgattttata aaaatattca agaaaagaaa catgggactg aactcgaagc
     5941 agaaattgat tggacagatt ctagggggaa tcatctttta tcttgtttac cgttctgaag
     6001 gttatccagg tgtattgaat ttcttcggac tagatctacc tttaggaatc atttatggta
     6061 ttttcgctct cttctggctt gttggatttt caaatgcagt aaatctgaca gatggcatcg
     6121 atggattagt tgctggctta ggaagtatct cttttgcagc ttacggtcta attgcttggc
     6181 atcagcatca gtatgatgta ttaatcattt gcttaagtgt tttaggaggt ttactgggtt
     6241 tcttcgtcta caacagaaaa cctgccaaga tttttatggg agatgtcggt tcattagcgt
     6301 tgggcggact tttagcggca atttctataa tgctaaacca agaatggaca ttgcttttga
     6361 ttggtttgat ctatgttatg gaaacagcaa gtgtgatgct tcaagttact tcctttaagc
     6421 ttacaggaaa acgaattttt aaaatgtctc caattcatca tcactttgaa atgtgcggat
     6481 ggtcggagtg gaagattgat acagtttttt ggttgatcag cattgtgact tcgttgatca
     6541 cattatggat cgtttggtag gagatgacgg gaaatgaaaa aaatatcaac atatgaaaat
     6601 aagaaagtgc ttgtcttagg actcgcaaaa agcggagtaa gtgcggccaa attattacat
     6661 gaattgggcg cacttgtcac cgttaatgat ggaaaacctt ttgacgaaaa tccggaagca
     6721 caagagctgc tttctttagg gataaaagta ataacaggta gtcatccaat cgaattgttg
     6781 gatgaagaat tttcattgat ggtcaaaaat ccagggattc cctacagtca tcctttagta
     6841 gcaaaagccc aagaaatggg tattccggta attacggaag ttgagctagc ttacgaagta
     6901 gcggaatgcc caattattgg gattacagga acgaacggaa aaacaacgac aacaacgatg
     6961 acaggactac tactgaatgc aggagcagat caaggaattg cacgtttagc gggtaatatt
     7021 gggtatccag caagtggtgt agcgcaagaa gcaaaatcag aagataaaat tgtgatggaa
     7081 ttgtctagct tccaactcat gggaatcaca gatttccgcc ctcatatagc ggtgattacc
     7141 aatatctatg aagcacatat tgattatcat ggtacaagaa aagaatatgt caaagctaaa
     7201 tggaaccttc aaaaaaacat gacagaaaaa gattatctga tccttaactg gaatcagtca
     7261 gaactacaag aattagcgca aagaacaaaa gcacgcgtac tgcctttctc gacaaaagaa
     7321 gtgttggaag acggtgtgta tgctgacgat tactcaatat actataaaaa agaaaaaata
     7381 atggaaatat ctgaacttgg cgtaccaggt aagcataatg tagaaaatgc actagctgct
     7441 atttctgtag caaaattgta tggtatatca aatgaagcta tacgagaaac gttgcatttt
     7501 ttccatggcg taccacatcg tacgcaatat gtaggagaaa tccaaggcag aaaattttat
     7561 aatgattcaa aagcaaccaa tattttagcg actaaaatgg ctctaagtgg atttgaaacg
     7621 tctaaggtcg tgttattggc tggcggatta gacagaggaa atacatttga tgaattaatt
     7681 ccttcattaa aaggaatcaa agcaatggtc gtatttggcc aaacaaaaga aaagttgatg
     7741 gatgctggaa aaaaagcagg catcgagaca atagtaacag ccgattctgt agaacaagca
     7801 gttcctttag cattagaaaa cagtacagat ggtgatgtag ttttattatc accagcgaat
     7861 gccagctggg atcaataccc aaattttgaa actagaggaa acagattcat ggaagctgtg
     7921 aaccgattaa aatagaaaaa gtagaagcga gcgataaaaa atgaaaattt tggttacagg
     7981 cggaggaaca gggggacata tttatccagc tcttgccttt gtcaattatg taaagactca
     8041 agaaccgaat gctgaattca tgtatgtggg agcaaaacga ggtctagaaa acaaaatcgt
     8101 ccctgataca ggaatgcctt tccatacctt ggagattcaa ggatttaaac gaaaaatttc
     8161 catgcataac atcaagacgg tccgattgtt tcttaagagt atccgtcaag caaaaaaaat
     8221 cctcagtgat tttcaaccag atatcgttat cggtactgga ggatatgttt ccggagcggt
     8281 agtgtacgct gcagccaaaa tgggtattcc aacagttatc catgaacaaa acagtgtacc
     8341 aggtgttacc aataaatttt taagccgata tgtggatcgg atcgctcttt catttaaaga
     8401 tgcggcttcc ttttttcctg aaaacaaagc agttcttgta ggtaatcctc gagcacaaga
     8461 agtagcagat actaaaaaat cagatatttt gaaaacattt gatttggatc cagaaaagaa
     8521 aacagtcttg atttttggcg gtagtcaagg agcacttaaa atcaatcaag cagtatcagt
     8581 ttttttaagt gaatatattc cttcttctta ccaagttttg tatgcttcag gagatagata
     8641 ttatgaagag atcctaaata aaataaatcc tgaaatacaa aatattagtg tccagcctta
     8701 tatcaaaaat atggcagagg tcatggcttc cagtgatctg ctggtggggc gtgctggtgc
     8761 aacttcgatc gcagaattca cagctctggg gttaccggca gtgttggttc ccagcccata
     8821 tgttacgaat gatcatcaga ctaaaaatgc aatgagtctt gttcacgcag gagcagcaaa
     8881 aatgattgca gataatgaac taacaggtga atcactaagt cagacagtca acgaaatcat
     8941 gggtgatgaa gaattgcaaa agcaaatgtg ccgagcttct aaagaacaag gaatccctga
     9001 tgcttcaaaa cgtctttatg acttagtaaa acaaataatc aaaaaataaa ggcggtggtc
     9061 ttagtgagta agaaaaaaga accaaaaaaa ttgtctcatc cgtcttctga tgaaaacaat
     9121 ttgactcctt ggcaaaaggc taaccgaaaa tatctggcag aacaagagat gaaaaaccaa
     9181 gagccaaaaa aagaagacat aacattagat acaccccttt cagattctga agaagaaaaa
     9241 acggttcaag aggaacagca agaagaaagc cagtccgaga aaaaaatcga attttttgaa
     9301 gagatcaagc aaaagaaaaa cggtggacca tataacggtt cttttttgaa ccggctgccc
     9361 aatttaaaaa gtcaacggca caaagtacta tttcgtcggt tagctttgat catcactgta
     9421 ttgggtatac cgctgatttt tttgatctat tatgtttctc cttatagcaa attacaagca
     9481 gttgcagtat caggcaataa gacagtcaac agccaagaaa tcatctctga tacaaaattg
     9541 tcattaggag aaaatgtttg gggacagtat tttcatcgat ctacttatat cgatcgcctt
     9601 aaaaaagcac agccaagaat cgaaacagca aatatacatt tcaaagggat gaatgaattc
     9661 gatttggatg taacagaata taaagagatt gctttgatcg caaaaaacaa tcaatactac
     9721 ccagtgattg aaaatggaac agtattagat gaaaaagtag caaatcctac aaaaaatctc
     9781 cctatcttgg aagagttcaa agactctgca aaaatcaaag aattaacaaa gcaatacaat
     9841 cagttatcat cagaattaca aaaagcaatt tctgaaatca agtacacgcc aagagcaagc
     9901 aataaaaatc tgatccaatt gaatatgaat gacggcaatc aagttattgt aaatatcaat
     9961 aatcttgcga accaaatgaa atattattcg caagtagcca aagatatgga tgaaaaaggt
    10021 gtgatcgaca tggaagttgg tatcttctcg catccttacg gtagcactca agaaacggaa
    10081 gaatcgggcc aatcggaaga aacaactgaa agtgaagaaa atgcggatca aacaaacgaa
    10141 tcaagtgaag aggcagaaac gcaaaaagca caaacacctg gatcgtctag cacaaacgga
    10201 tcatctgatt caaatgattc aagtgaaaca agtcaaatag aagataatcc aagtacaact
    10261 ccgtcaagtt taaatgaaaa ttgataaaac aactttaaaa agtaagaatg tactttctaa
    10321 aaaccagtaa tttatggtag aattggaag
//
LOCUS       DAZKLD010000104        10272 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.104, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000104 DAZKLD010000000
VERSION     DAZKLD010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10272)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10272)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10272
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.104"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1578)
                     /locus_tag="ACG8KR_002163"
     CDS             complement(<1..1578)
                     /locus_tag="ACG8KR_002163"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000149450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 protein"
                     /protein_id="HFQ0201494.1"
                     /translation="MTIKVDLRKKPYNLDEDAISWIEETIDSMTLEEKIGQLFVNMGS
                     SRDKNYLSNMVNQYHIGAVRYNPGTADEVYDQNYILQTESKIPLLIAANTEAGGNGAC
                     TDGTEVGVEVKIGATNDAKYAYELGRVSGIEASAIGCNWSFAPIVDINRNWRNPIISS
                     RSFGQNPDKVLELSLAYMKGIHESGILPAAKHFPGDGIDERDQHLSFSINSLGTEEWD
                     NSFGKVYSGLIEAGLPSIMAGHIHMPAYSKHFNPDLKDEDLLPATLSKELITDLLRGK
                     LGFNGLVVTDASHMLGMTGAMKRSELLPTSIAAGCDLFLFFNDPDEDFNYMMEGYRKG
                     IITDDRLLDALKRILGLKAKLGLHKKAKEQILLPKQEALEKIGLIENKSIFKEVADQA
                     ITLVKDKQDIWPIVPEKYKRILLVDVHGVKGGFGEVVAGNRPQAIDLLKQILSAKGFE
                     VEKYISPEEELKGLSQEEKIKNIAGIYAAKRPIKNLTKKYDLIINIANVNPGTVQRIV
                     WPASKGTPDIPFYIHEIP"
     gene            complement(1591..2385)
                     /locus_tag="ACG8KR_002164"
     CDS             complement(1591..2385)
                     /locus_tag="ACG8KR_002164"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFQ0201495.1"
                     /translation="MNNWIFCIDSDGCVMDTMTYKHNLFFGPIAAEIFDVHHREKFLQ
                     DWNNINLYSETRGINRFLGLVKTLEKNDVTSINRLKHWVSLTKELSNPSLKREIEYSQ
                     SEDLLKALKWSEEVNQQISQAKGLDKPFLSARDTIKALKKYGKIIIVSSANKEAVQEE
                     WERHELLSLVDELCCQDKGKKEDIIRSVIENGCDLDKILMIGDSPGDLEAANKNKVFF
                     YPILVNKEKESWENLRTDVLFKCLSGDYVDIEQKYIDTFWKNLTNK"
     gene            complement(2395..3771)
                     /locus_tag="ACG8KR_002165"
     CDS             complement(2395..3771)
                     /locus_tag="ACG8KR_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFQ0201496.1"
                     /translation="MERKFGIRDKIGYMFGDFGNDFTFIFASSFLMIFYTKVLGISGA
                     AVGTLFLVARFIDAVTDVTMGRIVDRSKATKQGKFKPWILRMSGPVALASFLMYQTSM
                     ISASMSVKVVYMYVTYILWGSIFYTSINIPYGSMASAITSITEERTMLSTFRNVGATL
                     ASLVIGVLTPLFIYTRDDSGAQIVRGGSTFTIVAGIFSICALICYLICYKLTTERVRI
                     DQSQYKVSLLKSLKGLVKNRALLSIIGAAIFLLLAQLLIMSMNNYVFPDYYNDAQGIV
                     LMNFINPILVLIVVSPLTLFLSKKYGKKELASVGMFFSAIVYLILFLIKASNMYVFLI
                     LSSVGYMGLGVFNTVIWANITDVIDDQEVKSDQREDGTVYAVYSFARKIGQALAGGVG
                     GWTLSIIGYDSLANVQTGDVLEKLYNASTLIPSICFLIVGLILFFLYPLSKDKVLENC
                     ELLKQKHK"
     gene            complement(3891..4538)
                     /locus_tag="ACG8KR_002166"
     CDS             complement(3891..4538)
                     /locus_tag="ACG8KR_002166"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-keto-4-hydroxyglutarate
                     aldolase/2-keto-3-deoxy-6-phosphogluconate aldolase"
                     /protein_id="HFQ0201497.1"
                     /translation="MKKVEILSKLEAAGVIAVVRGKNKDEAIKAINSIIAGGIKGIEL
                     TFTVPNAEEIIKEIAKKYADQKDVIIGAGTVLDAFTARIAIMAGAEYIVSPSFDRQTA
                     EICNLYQIPYLPGCMTITEIKEALTAGVDIIKLFPGSAFGPSIVKAYQAPLPHVNIMP
                     TGGVSIENLEDWFEVGVVAVGVGGNLLASVEKGDFEEVSRVASKYIDKFYEIKNY"
     gene            complement(4622..6049)
                     /gene="uxaC"
                     /locus_tag="ACG8KR_002167"
     CDS             complement(4622..6049)
                     /gene="uxaC"
                     /locus_tag="ACG8KR_002167"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HFQ0201498.1"
                     /translation="MFLDKNFLLENEIAKRLFHKFAKHQPIIDYHCHLDPAYLLENKQ
                     YKNLTQVWLNDKGAGDHYKWRLMRANGIEEELISGPGDDYEKFLAFVKTVEKAPGNPI
                     FEWSHLELQRYFDIDLTISEKNAPEIWRRANEKIQTDSYRPKELLEMMDVKAICTTDD
                     PTDDLWYHTELSKQDNSFKVLPTFRPDKVWSIKSSGFSNYILKLSQTAAIPIKDFNGL
                     KQALSQRVDHFHQIGCRLADHGLNTYFYEIADEKILDNLLIKALAGKTDFHLSEIAQF
                     VTGIQLHLMSEYTRYGWTMQMHLNALRNNSSILKEQIGVDVGGDSMGDQASLTQNVVA
                     LFDTAEKSGCLPKTILYSLNPTDWLPLATAMQSFQGGMIQKIQLGCAWWFNDTAEGMR
                     QQLTTMAQQSLLSNFTGMLTDSRSFLSYPRHEYFRRVLCQLLSEWVKAGRIPDDEMYL
                     SQIVENICYRNAYNYFNFFEIGSRK"
     gene            complement(6123..7202)
                     /gene="uxuA"
                     /locus_tag="ACG8KR_002168"
     CDS             complement(6123..7202)
                     /gene="uxuA"
                     /locus_tag="ACG8KR_002168"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFQ0201499.1"
                     /translation="MEMTFRWYGANEEKISLQEIRQIPAMKGIVGTLMDIPAGEVWPR
                     ERIKSLKDEVEANGLTLKVIESVNVHDDIKIGLPTRDQYIENYKQTIRNLAEFGIEVI
                     CYNFMPVFDWVKSDLDYRLDDGSSTLAYISKNIPTDPEEIIREVENSTNNFELPGWEP
                     HRLSQIKQLLEAYKEVDEEKLRENFAYFLKAIIPTCEEVGIKMAVHPDDPPYSIFGLP
                     RIVKNRDDLDWLCNVVDSPSNGITLCTGSIAERPDNDVYEILSEFCQRDRIPFAHVRN
                     IKYLSDNGLDFYEAPHKSEYGSLDMYKILKTMYDNGFNGYIRPDHGRMIWGETGRPGY
                     GLYDRALGAVYLNGIWEALEKGASK"
     gene            complement(7205..8836)
                     /locus_tag="ACG8KR_002169"
     CDS             complement(7205..8836)
                     /locus_tag="ACG8KR_002169"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFQ0201500.1"
                     /translation="MVSIKNWQNSIEEFNEMQILLPCFDVKEVKQEGQQHPVWLHFGG
                     GNLYRGFHAEIAQCLADQNELETGVVVCETFDEEVVEKAYHDYNNDILEIVMSGDGEL
                     KKRLLAVTADSLYCNPKNIADYQRVVKYFEDPKLQFTTFTITEKGYSLKDSKGQFFSV
                     VKADIKSGPSNPQHTMSIVTSLLYSRFNAGELPIAMVSTDNFSQNGQRFQDSIFTIAE
                     AWQKEGYVSEKFIAYLKDKEKVTFPWSMIDRITPNPSENVKEDLLMMGLEDLDIIHTK
                     KHTNIAAFTNTEVVHYLVIEDSFPNGRPKLEKAGVIMTDRETVDKADIMKVTTSLNPL
                     HTALAVTGCLLGYQSISAEMQDDDLVGLIKGIGYLEGLPVVENPGIIDPKQFIDEVIN
                     KRLPNPYIPDTPQRIATDTSQKVAIRYGETIKKYVEKDGNAENLTFIPIAIAAWLRYL
                     LAVDDQGENFVPSPDPLLVELQDKLSVLKLGEQDPSKIHCAVQEILENKEIFGLDLYQ
                     VGVGKKTEKIFCEMLVGPQAVRKTINKYITEVGGK"
     gene            9140..9811
                     /locus_tag="ACG8KR_002170"
     CDS             9140..9811
                     /locus_tag="ACG8KR_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFQ0201501.1"
                     /translation="MTIKTSLQNQAYQSIRRQIIYADLEPGKKVSEKKLEETLSIGRT
                     PIREALIQLRQQGLVDTIPQSGNYISLIDLDSAKNARFVREHLERQIMLECCAKMNNQ
                     KKRILETIISEQEKAVSQKNARSFFHNDNLFHKACFEIAEREEVWQWLENNNTHLERF
                     RWLRVKTEGLKWEKIMEQHYQLFEALTKQDPEEANFLTSLHLHLMLNEQNIVLATHPK
                     YFKNI"
     gene            complement(9971..10162)
                     /locus_tag="ACG8KR_002171"
                     /pseudo
     CDS             complement(9971..10162)
                     /locus_tag="ACG8KR_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010818872.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
BASE COUNT         3320 a         2021 c         1344 g         3587 t
ORIGIN      
        1 tggaatttca tgaatgtaaa acggtatatc cggagttcct ttacttgctg gccagacaat
       61 tcgttgaact gtgcctggat taacattagc tatattaatg atcaagtcat attttttagt
      121 taaattttta attggtctct tagcagcata tattccagct atatttttaa ttttttcttc
      181 ttgcgataat cctttcaatt cttcttcagg tgaaatgtat ttttcaactt caaatccttt
      241 tgctgacaaa atctgtttca ataaatcaat ggcttgaggt ctatttccag caactacttc
      301 accaaaccca ccttttaccc catgtacatc tactagtaaa atccttttat atttttctgg
      361 tacaataggc caaatatctt gtttatcttt aactaatgta attgcttggt cagccacctc
      421 tttaaaaatt gatttatttt caattaaacc aattttttcc agagcttctt gcttaggtaa
      481 taaaatttgt tcttttgctt ttttgtgtaa tcccaattta gcttttaacc ctaaaattct
      541 ttttaaggca tctaataacc gatcatcagt gataattcct ttacgatagc cttccatcat
      601 ataattaaag tcttcatctg gatcattaaa aaatagaaat agatcacatc cagctgcaat
      661 agaagtgggc aataattctg atcgtttcat tgccccagtc attcctaaca tatgactagc
      721 atcagtaact actaatccat taaatccgag ttttcctcta agtaagtcag tgattaattc
      781 ttttgataaa gttgcaggaa gtaaatcttc atctttcaga tcaggattaa aatgttttga
      841 ataagctggc atatggatat gacctgccat aattgaaggt aatccagctt ctattaagcc
      901 tgagtatact tttccaaaag aattatccca ttcttccgta cctaaactat taatactaaa
      961 agatagatgc tgatctcgtt cgtcaatacc gtcaccaggg aaatgtttag ctgcaggcaa
     1021 aattccactc tcatgaatac ctttcatata agctaaacta agttctaaaa ctttatcagg
     1081 gttttgtcca aaagatcgac tagaaatgat aggatttctc caatttctat taatatctac
     1141 aataggagca aaactccagt tacatccaat agccgatgct tcaatcccag aaactctccc
     1201 taattcataa gcatatttcg catcatttgt tgcaccaatt tttacttcta caccaacttc
     1261 agtaccatct gtacaagctc cgttaccacc tgcttccgta ttagcagcaa ttaatagagg
     1321 aattttactt tctgtttgta agatatagtt ttgatcatat acctcatcag cagttcctgg
     1381 attatatcgt acagctccaa tatggtactg attgaccata ttactcaagt aatttttatc
     1441 acgactagat cccatattaa caaacaattg acctattttt tcttccaaag tcatagaatc
     1501 aatcgtctct tcaatccaag aaatagcatc ttcatctaaa ttatatggtt tttttcgtaa
     1561 atctactttt atagtcattt tctttctcct ctacttatta gtaagatttt tccaaaaagt
     1621 atctatatat ttttgttcaa tatctacata atctcccgat aaacatttaa ataaaacatc
     1681 agttcttaag ttttcccatg attctttttc tttattgacc aaaataggat aaaagaaaac
     1741 tttattttta tttgcagctt ctagatcacc aggagaatca ccaatcatta atatcttatc
     1801 caaatcacaa ccattttcaa ttacacttcg aataatatct tctttttttc ctttatcttg
     1861 acaacacagt tcatcaacta atgataataa ttcatgtctt tcccattctt cttgaacagc
     1921 ttccttatta gctgagctta caataattat cttcccatat tttttcagag cttttatagt
     1981 atctctagca cttaaaaagg gtttatctaa tccttttgct tgactaatct gttgattgac
     2041 ttcttccgac cattttaaag cttttaataa atcctctgac tgagaatatt ctatttccct
     2101 cttcaaagat ggattagaca attcttttgt tagagatacc cagtgcttta atcgattaat
     2161 cgatgttaca tcattttttt ctaatgtttt aactaaacct aaaaatctgt ttattccacg
     2221 agtctctgaa tataaattaa tattattcca atcctgtagg aatttttctc tatgatgaac
     2281 atcaaaaatc tctgcagcaa ttggtccgaa aaagagattg tgtttataag tcatcgtatc
     2341 cataacacag ccatctgaat caatacaaaa tatccaatta ttcatatttc tctcctattt
     2401 atgtttttgt tttaaaagct cgcaattttc taagacttta tctttagata atggataaag
     2461 gaaaaataaa attaatccta caatcagaaa acaaattgat ggaattaatg ttgaagcgtt
     2521 atataacttt tctaaaacat ctcccgtttg tacatttgct aaagagtcat agcctataat
     2581 agaaagagtc caacctccga cgcctccagc tagagcttga cctatttttc tagcaaaaga
     2641 atataccgca tatacggtcc catcttctct ttgatcagac tttacttctt gatcatcaat
     2701 aacatcagta atatttgccc aaataacagt attaaacaca cccaaaccca tatagccaac
     2761 cgaactaaga attaagaata catacatatt actagctttt atcaaaaata agatcaaata
     2821 tactattgct gaaaagaaca ttcctactga tgccagttct ttttttccat attttttact
     2881 taaaaacaaa gttaaaggcg aaacaactat taaaaccaaa ataggattga taaaattcat
     2941 taaaactatt ccctgtgcat cattatagta atcaggaaat acataattat tcatactcat
     3001 aattaataat tgagcaagga gtaagaaaat agccgcacca attatagaaa gtaatgccct
     3061 atttttaact aaccctttta aagattttaa caatgaaact ttgtattgag attggtcaat
     3121 tcttactcgt tcagtagtta gtttataaca tattaaataa caaataagtg cacatatcga
     3181 aaatattccc gcaacaattg taaaagttga tccaccacga actatttgag cgcctgaatc
     3241 atctcttgta taaataaata atggtgttaa tacaccaatg accaaacttg ctaaagtagc
     3301 tccaacattt cggaatgtcg atagcattgt tctttcttct gtaatagaag taatagctga
     3361 tgccattgat ccataaggaa tatttataga tgtgtaaaaa atactccccc ataatatata
     3421 cgtaacatac atgtatacta ccttaacgct catagaagct gaaatcatag aagtttgata
     3481 cattaaaaaa cttgctaaag cgactggacc actcattctt aaaatccacg gtttaaactt
     3541 accctgctta gttgctttcg atctatcaac aatacgcccc atagttacat cagtaactgc
     3601 atcgataaat cttgctacta aaaataatgt tccaacggca gctccgctta tccccaatac
     3661 ttttgtataa aagatcatta aaaaagaact agcaaaaatg aaagtaaaat cgtttccaaa
     3721 atctccaaac atatatccta ttttgtctct tataccaaat tttctttcca tacaattctc
     3781 tcccttctgt tgttaagcgt tttcattctg atgaaagtac gtactatact ctgaagcgta
     3841 aagagtcagt atttattttt tacatttcag agtattattt gttttttctc ctaataattc
     3901 tttatctcat aaaacttatc tatatattta ctagctactc gacttacttc ttcaaaatca
     3961 cctttttcaa cagaagccaa taaattccca cctacaccaa ctgcaacaac tcctacttca
     4021 aaccaatctt ctaagttctc tatacttact ccgccagtag gcattatatt tacgtgtggt
     4081 aatggagctt gataggcttt tacaatggat ggaccaaaag cagaacctgg gaatagttta
     4141 atgatatcta cgccagcagt aagtgcctcc tttatctcag taattgtcat acaacctggt
     4201 aaataaggaa tttgatataa attacaaatc tcagctgttt gtcgatcaaa agatggacta
     4261 acgatatatt ctgccccagc cataattgcg atacgagcag taaacgcatc taaaaccgta
     4321 ccggctccga ttattacatc tttttgatca gcatattttt tagcaatttc tttgattatt
     4381 tcttcagcat taggaactgt aaaagttagt tcaatccctt taattccacc cgcaataatt
     4441 gaatttattg ctttaatggc ctcatcctta tttttcccac gtacaaccgc aatgactcca
     4501 gccgcttcta acttacttaa aatttctact tttttcatat ctatctcctt cctttatgga
     4561 tacatacttt gtctagctgt actaacttac aaagaataaa tttaagcttt tatttcaagt
     4621 attattttct acttccgatt tcaaaaaaat taaaataatt atacgcatta cgataacaaa
     4681 tattctccac tatctgacta agatacattt catcatctgg aattcggcct gctttaaccc
     4741 actcgcttag taattgacat aatactcgac gaaaatactc atggcgagga tagcttagaa
     4801 aacttcgaga atcagtcaac atacctgtaa aattcgacaa caaactttgt tgtgccatcg
     4861 tagtcaactg ttgacgcatt ccttcagctg tatcattgaa ccaccaagca catcccaatt
     4921 ggattttttg aatcataccc ccttggaagc tttgcattgc tgttgctagt ggaagccaat
     4981 cagttggatt taatgaatat aaaattgttt ttggtaagca cccactcttt tcagcggtat
     5041 cgaacaatgc tacaacattt tgtgtcaaac tagcttgatc ccccatcgaa tcaccaccga
     5101 catctactcc aatttgttcc tttaatatac ttgaattatt tcttaaagca tttaaatgca
     5161 tttgcatcgt ccaaccatat cttgtatact cagacatcaa atgtaattga atacctgtaa
     5221 caaattgtgc aatttctgat aaatggaaat ccgtttttcc tgctaaagct tttattaaca
     5281 gattgtcgag tattttttca tctgctattt cataaaaata agtattcaaa ccgtgatctg
     5341 ctaatcgaca gccaatttga tgaaaatgat ccactcgttg gctcaatgct tgcttcaacc
     5401 cattaaagtc tttgattggt atagccgctg tttgactaag ttttaaaata tagttcgaaa
     5461 agcctgatga tttgatactc cagaccttat ctggacggaa agtaggtaat accttgaaag
     5521 aattatcctg tttagaaagc tcagtatgat accacaagtc atctgtagga tcatccgttg
     5581 tacagatggc tttcacatcc atcatctcca acaattcttt cgggcgatag gaatccgttt
     5641 gaattttttc gttagcacgt cgccaaattt ctggtgcatt cttctcagaa attgtcaaat
     5701 caatatcaaa gtaacgttgt aattccaaat gagaccattc aaaaatagga ttacctggcg
     5761 ccttctccac tgtttttaca aaagctaaaa atttttcata atcatcacct ggaccactga
     5821 tcaactcttc ttctatacca ttagctcgca tcaatcgcca tttataatga tctccagctc
     5881 ctttatcatt taaccatacc tgagtcaaat tcttgtattg cttattttct agcaaatatg
     5941 ctgggtctaa atgacaatga tagtcgataa ttggttggtg ttttgcaaat ttatgaaaaa
     6001 gtcttttagc tatttcattc tctaataaaa agttcttatc taaaaacata tttaaacact
     6061 cctttccttc cgttagtttt tagaagagaa aatttttcct ctaaataaac tcccttatat
     6121 aattatttac tcgccccctt ttcaagcgct tcccaaatac catttaaata aactgcacct
     6181 agagctcggt catataatcc ataaccaggt ctaccggttt ctccccaaat catccgacca
     6241 tgatctggac gaatatagcc attaaatcca ttatcgtaca ttgtttttaa tatcttatac
     6301 atatctagag aaccatattc tgatttatgc ggcgcttcgt aaaaatcgag tccattgtca
     6361 cttaaatatt tgatattacg aacatgtgca aaaggaatcc gatcccgttg acaaaattca
     6421 gatagaattt catagacatc attatctggt cgttctgcaa tacttccagt acataatgta
     6481 ataccattag aaggagaatc cactacatta cacaaccaat ctaaatcgtc acgattttta
     6541 acaatacgag gtaatccgaa aatggaatat ggtggatcat ctgggtgaac tgccatttta
     6601 atcccaactt cttcacatgt cgggataatt gctttgagaa aatatgcaaa gttctctcgt
     6661 aatttctctt catcaacttc tttataggct tctaataatt gcttaatttg tgataaccga
     6721 tgaggttccc aacccggcaa ttcaaaatta ttagtagaat tttctacttc tctaataatt
     6781 tcctccggat cagtagggat attttttgaa atataggcta acgtagaaga cccatcatct
     6841 aaacgataat ccaaatccga ttttacccaa tcaaatactg gcataaaatt ataacagata
     6901 acctcaatcc caaattctgc caaattgcga atcgtctgtt tataattctc aatgtattga
     6961 tctcgtgttg gtaaaccaat tttgatatca tcatgaacat taacactctc aataactttc
     7021 aaggtaagtc cattggcttc gacttcatct tttaaagatt taattcgttc tctcggccaa
     7081 acctctccgg ctggtatatc cattaatgtt ccaacaattc ctttcattgc tgggatctga
     7141 cgaatttctt gtagtgagat tttttcttca tttgccccat accatctaaa agtcatttcc
     7201 atagttactt tcctccaact tctgttatat atttattgat tgttttacga actgcttgag
     7261 gacctactag catttcgcaa aagatttttt cagttttctt tcctacccca acttgatata
     7321 aatctaaacc aaagatttct ttattttcta aaatttcctg taccgcacaa tgaatctttg
     7381 aaggatcttg ttcacctaat ttcaatactg ataatttatc ctgcaactct accaataacg
     7441 gatcaggact tggcacaaaa ttttcccctt gatcatcgac tgccagtaaa taacgtaacc
     7501 aagcagcaat agcgatagga ataaatgtta gattttctgc atttccgtct ttttcgacat
     7561 actttttaat tgtttcacca tagcgaatcg ctactttttg agaagtatct gttgcaattc
     7621 gttgtggtgt atctggaata tagggattag gcaaacgttt attaattact tcatcaataa
     7681 attgttttgg atcaataatg cctggatttt caactactgg taaaccttct aaataaccta
     7741 ttcctttgat taaaccaacc aaatcatcat cctgcatctc tgctgaaatt gattgatatc
     7801 ctagcagaca acctgttact gccaaagcag tatgaagtgg attcaagctt gtcgtaacct
     7861 tcatgatgtc tgctttatca accgtctctc tatctgtcat aatcacacca gccttttcca
     7921 atttaggccg gccatttgga aaactatctt caataactaa ataatgaact acctcagtat
     7981 tagtgaaagc agcaatattg gtatgttttt ttgtgtgaat gatatctaaa tcctcgagcc
     8041 ccatcatcaa tagatcttcc tttacattct ctgaaggatt tggcgtaatt cgatcaatca
     8101 tactccaagg aaaagtcact ttttctttat cctttaaata agcaataaat ttttcagaaa
     8161 catatccttc tttttgccaa gcttctgcaa tcgtaaaaat actatcttga aaacgttgac
     8221 cattctgtga gaaattatca gtagaaacca tcgcaatcgg caactctcca gcattaaatc
     8281 tgctatataa caaggatgtt acaatactca tcgtatgttg aggattgcta ggtccagatt
     8341 tgatatcagc tttgactacc gaaaagaatt gccctttaga atcttttaaa ctgtaacctt
     8401 tttcagtaat agtaaacgtt gtaaactgta atttaggatc ttcaaaatat ttaaccactc
     8461 tttgatagtc cgctatattc ttcggattgc aatataaact atcagccgtt actgccaaaa
     8521 gtcttttttt gagttcacca tctccactca taacaatttc caaaatgtca ttgttatagt
     8581 catgataagc tttttccact acttcttcat caaatgtttc acacacaact actcctgttt
     8641 ctaattcatt ttgatctgct aagcattgag caatttctgc atgaaatcct cgataaagat
     8701 ttcctccacc aaaatgaagc catactggat gttgctggcc ttcttgctta acttctttga
     8761 catcaaaaca cggcagtaga atctgcattt cattgaattc ttcgatagaa ttttgccaat
     8821 ttttaatcga aaccataatt aacctcctca aactgatatg ttagttttga tacattttat
     8881 attttaaaac ccattgtaac cgttgtcaat ataaaaaggg aataaacttg cttttttcta
     8941 ccatatcaga atgattgtat tttttattca gaaaaataaa atactccttc tttttggact
     9001 gattttatcg aaacttattt tttctttttt tcatgtattc ttttattttt ataactagtg
     9061 tattacttca aaatttgata ttatatggca tgaacagaat taaaatatca gttaaaataa
     9121 aagttaagga gcattgtgta tgacaattaa aactagtctc caaaatcaag cttaccaatc
     9181 tattcgccga caaattatct atgctgactt agaacctgga aaaaaagtat cagaaaaaaa
     9241 attagaagaa acactttcta ttggtcgtac accaatacgt gaagcactga ttcaactccg
     9301 acaacaaggt cttgttgata caattcctca aagcggaaat tatatctcat taattgattt
     9361 agattcggcc aaaaatgctc gatttgtcag agaacatttg gaacgacaaa ttatgttaga
     9421 gtgttgtgcc aaaatgaata accaaaaaaa acggatttta gaaactataa tttcagaaca
     9481 agaaaaagct gttagtcaaa aaaatgctcg tagttttttt cataatgata atttattcca
     9541 taaagcttgt tttgaaattg cagagcgaga agaagtatgg caatggttag aaaataacaa
     9601 tacacactta gaacgatttc gatggctacg agtaaaaacg gaaggattaa agtgggaaaa
     9661 aattatggaa caacattatc aactatttga ggcattaaca aaacaagatc cagaagaagc
     9721 taactttcta acatcgttgc atcttcattt aatgcttaat gagcaaaata ttgttttagc
     9781 tactcatcca aaatatttca aaaatatata acttttaact ctttttaaat taaatggttg
     9841 ttctgatcag aaaaatatcg cttgttttct tattatgtaa tatatttcat aaactattct
     9901 gcataacaac tattgtttgt actttttaca ttggataact aattatgact gctgataact
     9961 tttgatatta aactagtatc tccaatacaa ataaaaaata gatatcttgt tcatcaaaat
    10021 tagttcgtgg atacttacct tcatttacat cattcagcgg tttcccattt tcttttattc
    10081 tattatttca ttttctaata gacttggctt catttcgcaa tacttttttt caataggcat
    10141 caaattggtg ttgtttagat atttagttgt agttttgtga catgataagt atgctccttt
    10201 ttctcttttc cactaataca acgatcaaag ggtgcttttt tcaaaaaact agaaaaatca
    10261 aaataagatt cg
//
LOCUS       DAZKLD010000105        10243 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.105, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000105 DAZKLD010000000
VERSION     DAZKLD010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10243)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10243)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10243
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.105"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            175..324
                     /locus_tag="ACG8KR_002172"
     CDS             175..324
                     /locus_tag="ACG8KR_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HFQ0201502.1"
                     /translation="MNKWRSNPKLNYWGKFIIRTIFYSAVLLFLIYLYHYKNIQGGTF
                     IYNEF"
     gene            335..1849
                     /gene="dltA"
                     /locus_tag="ACG8KR_002173"
     CDS             335..1849
                     /gene="dltA"
                     /locus_tag="ACG8KR_002173"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047473 - D-alanine [D-alanyl carrier
                     protein] ligase activity [Evidence IEA];
                     GO_process: GO:0019350 - teichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HFQ0201503.1"
                     /translation="MEIKTIIEAIDDWGINEPDRVAYQADETHTFGELKQASDALAYY
                     LEKKVEGDGPIVVFGNLEFEMIVSFLGVVKSGHAYLPIEEHTPKERILSIFRVAKPSM
                     VISIGDWIEDIPSVPVITKKEFQEIKKIPVGFYPRNSVKGTENFYIIFTSGTTGEPKG
                     VQISHDNLVSFVQWTMEDFGIKPGMHFLAQAPFSFDLSVFSIYPALVSGGMLKPLNKA
                     VVQDFRQLFATLPALKLNVWVSTPSFMDICLMEPTFNGENVPELDMFLFCGEELTKKT
                     AESLLDRFPNARIYNTYGPTEATVAISSIQIDQHVLDSYDRLPIGYVKKDTKVSIVQE
                     GKPVSKGETGEIIIAGPSVSKGYLNNPEKTAAAFFCQNDLASYHTGDAGRLDEYGLLF
                     YEGRMDQQVKLHGYRIELGDIEHYLLQDNRIKQAVVVPKYQGTKVQQLVAFVVLENKT
                     KQPDFQLTKSIKEQLLEVVMDYMIPQKFHYVDLLPQTVNGKIDRKKLTAEVNPE"
     gene            1846..3051
                     /gene="dltB"
                     /locus_tag="ACG8KR_002174"
     CDS             1846..3051
                     /gene="dltB"
                     /locus_tag="ACG8KR_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0070395 - lipoteichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HFQ0201504.1"
                     /translation="MMLPHMIPYAEPFYFFLLAIALVPIVLSLLIWEKRLPVYQSAVT
                     LFFLFVSFGGNYWQQGAALIGYVLWQTILVWGYFVYRQRKNQTGWFFAAVILAIIPLF
                     LTKVTPFFEHGKASLLGFLGISYLTFKSVQMIMEIRDGMIKTYKPFRYIQFLLFFPTI
                     SSGPIDRYRRFEKDQLHPPVRDKYVDLLGKGVQNIFVGFLYKFIIGYYFGQVLLPVVG
                     RIALQHGGISWALVGYMYVYSMYLFFDFAGYSLFAVGTSYMMGYQTPINFNKPFLSWN
                     IKEFWNRWHMTLSFWFRDYVYMRLMFFMMKKKFFKSKIVMSNIGYFALFLLMGIWHGL
                     TWYYIVYGLYHACLICLTDAWIRFKKKHKEQLPSNKFTHGLAVFLTFQAVCFSFLIFS
                     GFLDKLFFS"
     gene            3096..3329
                     /gene="dltC"
                     /locus_tag="ACG8KR_002175"
     CDS             3096..3329
                     /gene="dltC"
                     /locus_tag="ACG8KR_002175"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036370 - D-alanyl carrier activity
                     [Evidence IEA];
                     GO_process: GO:0019350 - teichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HFQ0201505.1"
                     /translation="MEEQILSILEEITGTDEVRKNRDIDLFDEGLLDSLASVQLLVEL
                     DGELDIQVPVSEFEREDWSTPNKIIQQATALKG"
     gene            3332..4600
                     /gene="dltD"
                     /locus_tag="ACG8KR_002176"
     CDS             3332..4600
                     /gene="dltD"
                     /locus_tag="ACG8KR_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070394 - lipoteichoic acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HFQ0201506.1"
                     /translation="MKKKLFAIFGPVVLAVGLLFVFFTSSYQVDISNPETIRRASSSM
                     SVNVLKGDAIKNYALSEKQYIPFFGSSELSRISPFHPSVLAEKYQRNYRPFLLGAPGT
                     QSLSQYMMMRSAGDAMKNKKVVFIISPQWFVKNGVKTDYFNTYYSELQTYDWLFSMKK
                     VTPADRYLARRLLTFSKVKENDTLTAILQTIKKGKLPLPESLNQLRSQWNMLKREDEV
                     FSNIGLKDRQQKIDHESKRLPKQYQETELSILANQIGERETTNNPFGLKNDFYTHRIR
                     AHEPELKQSQKNWDYRFSPEFSDFQLVLDQLAKNHNEVLFIIPPVNEKWSDYTGLSQE
                     MLQGFAKKIKFQLNSQGFNRIADFVNQAGTNYFMEDTIHLGWKGWLAADQQIRPFLEE
                     NHITASKYHLDDAFFSKSWQHQIPDKLQLK"
     gene            4741..5823
                     /locus_tag="ACG8KR_002177"
     CDS             4741..5823
                     /locus_tag="ACG8KR_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HFQ0201507.1"
                     /translation="MDTKEEQLLIDLTSAKGVPGNEEEVREVFREYAKPFADDIFYDG
                     LGSVIAKHGAGGGPKVFISGHMDEVGFMVTKITEKGFLEFQTLGGWWGQVMLAQQVEI
                     KTREGKIVHGVIGSKPPHVLTPQVRNKPYEIKDMFIDIGASSQEEAKEWGIRPGDMVT
                     PYIEYKRMNGSKYLLAKAWDNRIGTAVSLRVLENLSKEAHPNVLFAGSDVQEEVGLRG
                     ARTSTHLVNPDIAFALDTGTAGDTPGMTPKEADSILGKGPQILIFDASMIPHKKLLNF
                     VIDVAEEMDIPFQYTVISGGGTDAGQMHLTRDGVPSVAITVPVRYLHSHTSVIHEDDY
                     LNTVKLVTELVKRLDSETVEKIRSYE"
     gene            5823..6626
                     /locus_tag="ACG8KR_002178"
     CDS             5823..6626
                     /locus_tag="ACG8KR_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HFQ0201508.1"
                     /translation="MNNRIPISEYRKKARVSLENQWGINTWLVFLSIIFTSVIQNFFD
                     GFSPFPEESMQQHLLSFLVDNLLLFAFNYALFYIALVVVRGGRPKSNLLFSVFQRKYF
                     GSILGINLINSVINWLLNSLIFLPMFLISGVSTYMQLLFSSNAVPTGVMPEDFLDISF
                     VLSMIGMIFVSALIMSIVGGLFQFAAWTKFDYPDLSVIQCLKYGWFLLKDRIGTYILL
                     QLSFIGWYLLGAIALFIGLLWVIVYVNVSVAELYEQARIEKGGPDAYFT"
     gene            6696..7436
                     /locus_tag="ACG8KR_002179"
     CDS             6696..7436
                     /locus_tag="ACG8KR_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFQ0201509.1"
                     /translation="MKLTVLGCLGAYPYKNQGTTGYLLQSDGFNLLIDAGSATLIKLQ
                     EHLDPLDLDAVILSHYHHDHIADLGVLQYYWQLHPKRNPETLLSIYGHTKDSIHFEEL
                     TLENVSKGHPYFEAEELKIGPFLITFMETIHPVVCYAMRIVEETTGKVFVFTGDSGYL
                     ESFADFAKDADLFLADTYLFEGNEHHHAHFTSKEAGELAKSANVKKLVLTHLPQFGDL
                     EQLKNEAQKAAGKGIDIELAAVDKVFNI"
     gene            7447..8451
                     /locus_tag="ACG8KR_002180"
     CDS             7447..8451
                     /locus_tag="ACG8KR_002180"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017118 - lipoyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HFQ0201510.1"
                     /translation="MIFVPNENHDPRINLAIEQYLLQEMPLDEPILLFYINEPSIIIG
                     RNQNTLEEINRDYVEENGIHVVRRLSGGGAVYHDFGNLNFSFIMPDDGDSFRDFAKVT
                     QPIIQALHELGVAGAELKGRNDLVIDGMKFSGNAMYATNGRMFAHGTIMFDSDINEVV
                     NALKVKKDKIESKGIKSIRSRVTNIKPFLPEEKQKMTTEEFRQEILLKIFGVESVEEV
                     KTYELTDEDWKKINRISEEYYRNWDWNYGKSPDFNFSRQKRFSIGSIEVHLNVSEGMI
                     KEAKIFGDFFGLGDISDVEKKLVGQKYDKASLSKIVSQIDVKKYFGAIEPEELLTLLY
                     "
     gene            complement(8496..10241)
                     /locus_tag="ACG8KR_002181"
     CDS             complement(8496..10241)
                     /locus_tag="ACG8KR_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0201511.1"
                     /translation="MAYFFYFIFSPADSNQMKEIKDSWVFYTKDDPDFKFDSKYTRTI
                     PTVDKDETFIMETTLEKPLDEANLLIKGNHQWITVYLGEKILYQRKDYQEESNPGLSL
                     AVIDLPANYIGKKLTLTVSSPYQNYAGLPPKVYLGTTGPLISFIYSQSVPQVLTMIIA
                     VFIAIGTFFYTIYLMYKQKRLEYSLILLSCFALALGFEAVSEDILSGILFEPFVHSFL
                     SHLFIILTSAFIICYYWSRMFYYKKWYGIFCTFQLLIFIGVLLYATFTPAELPELMPI
                     ANIASVISTLVTSLACIGEAYKNNRFYVACTPWIVLVAIIHCLIYIQTALGIYQTTIN
                     WSTILYIIILIVIISYNLIDHILNTDKDRRQVDFLKIKNDLLEQHYEQLRQHIQEVNT
                     LRLEFVQEMENLQDLMHTDQTKAKNYVEKILEEAKNFEMLCSFCNHQVTNLILARYQK
                     LAAKRNIQITFQADLPEELPINDDDLAQLLIHALEHSFRETHAIENPLYRKIYLSIHE
                     QEEHFVICCEHSAHYDTNLFSRGITEELDDQERFDLMMMKDVADRYTGTLTQEKDTFI
                     DRITVQLSRNYPNPV"
BASE COUNT         3288 a         1695 c         2080 g         3180 t
ORIGIN      
        1 ttttttaaaa gtacgagaaa ttataaagaa aaaagcagtg atttgatgtg ctatttttat
       61 aaaagctatg tgacaaaact gttagtaaaa aaacggtttc tgtaagctag acacatgttt
      121 ctttttgtca aaattagtta cactttgtgt gaggttatat gaggagggaa tccaatgaac
      181 aaatggcgtt ctaatccaaa attgaattat tggggaaaat tcatcattcg aacaattttt
      241 tatagtgcag tactgctgtt cctgatttat ctgtatcact ataaaaatat tcaaggagga
      301 acttttatct ataacgaatt ttaggaggaa gagtatggaa atcaaaacga ttattgaagc
      361 aatcgatgat tggggaataa atgaacctga tcgagttgct tatcaggcgg atgagacgca
      421 tacttttggt gaattgaaac aagcatcaga cgcacttgct tattatttag aaaaaaaggt
      481 agaaggcgat ggaccaattg tcgtttttgg caatttggag tttgaaatga ttgtctcatt
      541 tctaggggtt gtcaaatctg gccatgccta tttgccgatt gaagagcata cacccaagga
      601 gcgtatccta tcgatctttc gggtggctaa accatctatg gtcatcagta tcggggactg
      661 gatagaagat attccttcgg ttcctgtcat aacaaaaaaa gaatttcaag aaatcaagaa
      721 gataccagta ggattttatc ccagaaatag cgtaaaaggt acagaaaatt tttatattat
      781 ttttacttct ggtactactg gagaaccaaa aggcgtacaa atcagtcatg ataatctagt
      841 cagctttgtt caatggacga tggaagactt tgggatcaag cctggtatgc atttcttagc
      901 ccaagcgcca ttttcttttg atttatcggt attttctatt tatcctgcat tagtttctgg
      961 cggaatgctg aagccgttaa ataaagcagt tgttcaagac tttcgtcaat tattcgcgac
     1021 attgccagcc ttgaagttga atgtgtgggt ttctactcca tcatttatgg atatttgctt
     1081 gatggaacct acattcaatg gagaaaatgt gccggaattg gacatgtttt tattttgcgg
     1141 agaagaattg acgaaaaaaa ctgcagaaag tctacttgat cgattcccaa atgcacggat
     1201 atataatact tacggtccaa cagaagcaac tgtagctatt tcaagtattc agattgacca
     1261 gcatgtacta gatagttatg accgactacc gattggctac gtaaaaaaag acacgaaagt
     1321 ttctattgtc caagaaggta aacctgtttc aaaaggagaa acgggtgaaa tcattattgc
     1381 aggaccaagc gtatcaaaag gttacctgaa caatcccgaa aagacagctg ctgcgttctt
     1441 ttgtcaaaat gacttagcaa gttaccatac aggggatgca ggtagactag atgaatatgg
     1501 gcttttattt tatgaaggac gtatggatca gcaggtcaaa ttacatggat accggattga
     1561 actcggggat atcgaacatt atctgctcca agacaaccga atcaaacaag ctgtcgtcgt
     1621 accaaaatac caaggaacaa aagtacagca gctggttgct tttgtcgtat tagaaaacaa
     1681 aacgaaacag ccggattttc agttgactaa atctatcaaa gaacaattac tagaagttgt
     1741 aatggattat atgattcctc agaagttcca ttatgtcgat ctgctgcctc agacagttaa
     1801 tggaaaaatc gatcgaaaaa aattaacagc tgaggtgaat cctgaatgat gttacctcat
     1861 atgatccctt atgcagagcc gttttatttt tttcttttag ccattgcgct tgtaccgatc
     1921 gttctatctt tattgatatg ggaaaaaaga ttaccagttt atcaatcagc agtgacactt
     1981 ttctttttat ttgtcagctt tggaggtaat tattggcaac agggagcggc gctgattggg
     2041 tatgttttgt ggcagacgat acttgtatgg ggctattttg tttatcggca aaggaaaaat
     2101 cagacaggat ggttttttgc cgccgttatt ttagcgataa ttccactgtt tttaacgaaa
     2161 gtgactcctt tttttgaaca tggaaaagca tcgttacttg gttttttagg aatctcttat
     2221 ttaaccttta aatctgtaca aatgatcatg gaaataagag atggcatgat caaaacatat
     2281 aagccatttc gttatatcca gtttctgctg ttctttccaa caatctcttc tggaccaatc
     2341 gatcgctata ggagatttga aaaagatcag ctccatccgc ctgttcgtga taaatatgtt
     2401 gatcttttgg gaaaaggcgt ccaaaatatt tttgtcggtt ttttatataa attcattatc
     2461 ggttattatt ttggtcaagt actgcttcct gtagtgggac gcattgcctt acagcatggt
     2521 ggtatttcat gggcattagt aggttatatg tacgtgtaca gtatgtattt gttttttgat
     2581 tttgctggct atagcttatt tgcagtcggt acaagctaca tgatgggata tcagacacca
     2641 atcaatttca ataaaccttt tttatcatgg aatatcaaag aattctggaa tcgttggcat
     2701 atgacgctgt ccttttggtt ccgtgattat gtatatatgc gattgatgtt cttcatgatg
     2761 aagaaaaaat tttttaaaag caagatcgta atgtcaaata tcggttattt tgctttgttt
     2821 cttctaatgg gtatatggca tggattgacg tggtactaca tcgtatatgg actgtatcat
     2881 gcttgtctga tctgtctgac agatgcatgg atccgattta agaagaagca taaagagcag
     2941 ttgccttcta acaaatttac gcatggctta gccgtatttt tgacatttca agcagtatgt
     3001 tttagcttcc tgattttttc aggattttta gataaacttt tttttagcta acgaatagtg
     3061 acatattaat ttatataaca aaggagaaaa ggaaaatgga agaacaaatt ttatcaatct
     3121 tagaagaaat aacaggaaca gatgaagtaa gaaaaaatcg tgatattgat ctatttgatg
     3181 aagggttgct tgattcttta gcatctgttc aattattagt cgagctagac ggagagctgg
     3241 atatccaagt ccctgtatct gaattcgagc gagaggattg gagcacaccg aataaaatca
     3301 ttcaacaagc aactgcattg aagggttgag tatgaaaaag aaactttttg ctattttcgg
     3361 cccggttgtt ttagctgttg gcttactgtt tgtgtttttt acctcctcct atcaagtaga
     3421 tatctctaat ccagaaacga ttagacgtgc ctcttcttcc atgtcagtga acgtgttgaa
     3481 aggagatgcg ataaaaaatt atgcattatc tgaaaaacaa tatattcctt tttttggctc
     3541 aagtgaatta agcagaatca gtccatttca tccgtctgtt ctggcagaaa aataccagcg
     3601 aaactatcgt ccatttttgc tgggagcacc cgggacacaa tcgttaagcc aatatatgat
     3661 gatgcgttct gcaggagatg ctatgaaaaa caagaaagtc gtctttatta tttctcctca
     3721 gtggttcgtt aaaaatggtg taaaaactga ttactttaat acgtattatt ctgaacttca
     3781 aacatacgac tggttgtttt caatgaagaa agtaacgcct gctgatcgct atttagctag
     3841 aagattatta actttctcaa aagtaaaaga gaatgatacg ttgacggcga ttctgcaaac
     3901 gatcaaaaaa gggaagctac ctttacctga atctttgaat cagctgcgca gccaatggaa
     3961 catgctgaag agagaggatg aagtattcag caatattggg ttgaaagatc gacagcaaaa
     4021 aatcgatcat gagtcaaaac gattaccaaa acaataccaa gaaacagaat tatccatttt
     4081 agcaaatcaa attggagaac gagaaacgac gaataatcca ttcgggttga aaaacgattt
     4141 ttatacgcat cgtatccgag cccacgagcc agagctcaaa cagtcacaaa aaaattggga
     4201 ttatcgtttt tcgccagaat tttctgattt tcagctggtc ttggatcaat tagcaaaaaa
     4261 ccataacgaa gtattgttca tcatcccacc ggttaacgag aaatggagcg attataccgg
     4321 actatctcaa gaaatgttac aaggatttgc caaaaagatc aaatttcaat taaatagcca
     4381 aggattcaat cgaatagctg attttgtcaa tcaagcgggt accaactatt ttatggaaga
     4441 cacgatccat ctaggctgga aaggctggtt ggctgcagat caacagatca gaccgttttt
     4501 agaagaaaat catattactg ctagtaagta tcatcttgac gatgcttttt ttagcaaatc
     4561 ttggcaacat caaataccag ataaactaca attaaaataa agaaaacagg cgagtgttgc
     4621 tcgcttgttt tctttatttt tgaatattct gaataaaaga taaaaatacc aagtttgcct
     4681 gaacaaaaat cataagcaat ggtataatca caatatgaat atagaaatgg gggaaaacat
     4741 ttggatacaa aagaagaaca gctacttatt gatttaactt ctgcaaaagg agtacctgga
     4801 aatgaagaag aggtaagaga agtattcaga gaatatgcca aaccattcgc tgatgatatt
     4861 ttttatgacg gtttaggcag tgtgattgcc aaacatggtg ctggcggagg ccccaaggtc
     4921 tttatttctg gacatatgga cgaagttggt tttatggtaa caaaaataac cgaaaaagga
     4981 ttcttggaat tccaaactct tggaggttgg tgggggcaag tcatgcttgc tcagcaagtt
     5041 gaaatcaaaa cacgtgaagg gaaaatcgtc cacggtgtga ttggttccaa accaccacat
     5101 gttttaacac ctcaagtaag aaataaacct tatgaaataa aggatatgtt cattgatatt
     5161 ggcgcatcaa gccaagaaga agccaaagag tggggcatac gtcctggaga tatggtcact
     5221 ccatatattg aatacaaacg aatgaatggc agtaaatatt tactggcaaa agcatgggat
     5281 aatcgtatcg ggacagctgt ttctctgcga gtattagaga atctatctaa agaagcacac
     5341 ccaaatgttt tattcgcagg aagcgatgtg caagaagaag taggcttgcg aggtgctcga
     5401 acaagtacac accttgtaaa tcccgatatt gcttttgctt tagatactgg tacagcagga
     5461 gacactccag gaatgacacc aaaagaagca gattcgattt taggaaaagg cccacagatt
     5521 ttgatttttg atgcatctat gatcccgcat aaaaaattat tgaattttgt tattgatgta
     5581 gcagaggaaa tggatattcc attccaatat acagtgattt ctggcggagg aactgatgca
     5641 ggacaaatgc atttgacaag agatggtgtt ccgtctgtag ccattaccgt tcctgttcgt
     5701 tatctgcatt cccatacatc agtgattcat gaagatgatt atttgaatac agtgaaacta
     5761 gtcacagaat tagtgaaacg gcttgatagt gaaacagttg aaaaaattcg gagttatgaa
     5821 taatgaataa tagaatacct atttctgaat atcggaaaaa agcacgagtt tctttggaaa
     5881 atcaatgggg aatcaatact tggcttgttt tcttatctat aatatttacg agcgtcatcc
     5941 agaacttttt tgacggtttc agtccttttc cggaagaaag catgcagcag cacttattga
     6001 gttttctagt cgataattta cttttattcg cattcaacta tgcattgttt tacattgcgc
     6061 tagtagttgt acggggtggc agaccaaaaa gtaacctttt attttcagtt ttccaaagaa
     6121 agtattttgg ttcgatactt ggcatcaatt tgatcaattc agtcatcaac tggctactca
     6181 acagtttaat ctttctgccc atgtttttaa tcagcggagt gagtacttat atgcagctgc
     6241 ttttcagttc aaatgcggta cctacgggtg tcatgccaga agattttctt gatatcagct
     6301 ttgttttatc gatgataggg atgatttttg tttcagcact catcatgagt attgttggcg
     6361 gtttattcca atttgctgca tggacaaagt tcgactatcc tgatttgtcg gttattcaat
     6421 gtttgaaata tggctggttt ttattgaaag atagaattgg tacgtatatt ttgttgcagc
     6481 tgtcttttat cggctggtat ttgctaggag caatcgcttt gttcatcggt cttttgtggg
     6541 tcattgttta tgtgaatgtg tcagttgcag aattatatga acaagcacgg attgaaaaag
     6601 gaggtccaga cgcctatttt acttagcatg taagcagttt attcatcaaa aaaataactt
     6661 ttgtggtagg ataaatcttt gagggggaat gtacgatgaa attaactgtt ttaggatgct
     6721 taggagcgta tccttataaa aatcaaggga caacaggtta tttgcttcaa tctgacgggt
     6781 tcaatttgtt gatcgatgca ggtagtgcta cgctgatcaa attacaagaa caccttgatc
     6841 cacttgatct agatgctgtg atccttagcc attatcatca tgatcatatt gctgaccttg
     6901 gtgtattaca gtattactgg caacttcatc ctaagagaaa tccggagaca ttgctttcaa
     6961 tttatggaca cacaaaagat tccattcact ttgaagaatt gacgttggaa aatgtctcaa
     7021 aaggacatcc ttattttgaa gctgaagaat taaaaattgg tccttttctg attactttta
     7081 tggaaacaat ccatccagta gtatgttacg ccatgcgtat cgtagaagaa acaacgggga
     7141 aagtattcgt atttacaggt gattcaggtt atttggaaag tttcgctgat tttgcgaaag
     7201 atgctgattt gtttctagca gatacctatc tttttgaagg aaatgaacat catcatgccc
     7261 atttcacttc aaaagaagct ggagaacttg cgaaatcagc taacgtaaag aaacttgtac
     7321 ttacacacct acctcagttt ggtgatttag aacaattgaa aaatgaagcg caaaaggcag
     7381 caggcaaagg gattgatata gaacttgctg cagttgacaa agtgtttaac atttaggagg
     7441 aagaacatga tttttgtacc aaatgaaaac cacgacccaa gaatcaattt agcaattgaa
     7501 caatatttgc tgcaagaaat gcctttagat gaacctattt tgctctttta tatcaatgaa
     7561 ccttcaatca tcatcggcag aaaccaaaat acgcttgaag aaatcaatcg agattatgtg
     7621 gaagaaaatg gcattcatgt tgtccgtcgc ttaagtggag gcggagctgt ctaccatgat
     7681 tttggaaacc tgaactttag ttttatcatg cctgatgatg gggattcatt ccgtgacttt
     7741 gcgaaagtaa cacagccaat catccaagca cttcatgaat tgggtgttgc tggggcggaa
     7801 ttgaaaggcc gaaatgatct agtcattgat ggcatgaaat tttctggcaa cgcgatgtat
     7861 gctaccaacg gtcggatgtt tgctcatgga acaattatgt tcgactctga tatcaatgaa
     7921 gtagtcaatg cattaaaagt aaaaaaagac aaaatcgaat caaaaggaat caaatcgatc
     7981 cgttctcgtg taacgaatat caaaccattt cttccagaag aaaagcaaaa aatgacaaca
     8041 gaagaatttc gtcaagaaat cttgttgaaa attttcggcg tagagtcagt agaagaagta
     8101 aaaacgtacg aattgactga tgaagattgg aaaaaaatca atcgaatctc tgaggaatat
     8161 tatcgaaatt gggattggaa ttacgggaag tctccagatt tcaatttctc tcgccaaaaa
     8221 cgtttttcta tcggttcgat tgaagtccat ttgaatgtct cagaaggaat gatcaaagaa
     8281 gcaaaaatct ttggcgattt cttcggacta ggagatatct ctgacgtgga aaaaaaattg
     8341 gttggacaga aatatgataa agcatctcta tcaaaaatag tcagccaaat tgatgtgaag
     8401 aaatattttg gtgcgataga accagaagaa ttgcttacct tactttattg aaaataaaaa
     8461 atagcggaat ccacatatat ggattccgct attttttata caggattcgg gtaatttcga
     8521 gagagttgaa cagtgatccg atcaatgaaa gtatcttttt cctgcgttag tgttcctgtg
     8581 taacgatccg ctacgtcttt catcatcatt aagtcaaaac gttcctgatc atctaattct
     8641 tctgtgatgc ctcgactaaa taggtttgta tcgtaatgtg cactatgttc gcaacaaatg
     8701 acgaaatgtt cctcttgttc gtgtatcgac aaataaattt ttcgatacag tggattttcg
     8761 atggcatgag tttctctaaa ggaatgctct aaagcgtgga tcaaaagttg agccagatcg
     8821 tcatcgttga taggaagttc ttcgggaaga tctgcttgga aggttatctg aatattccgt
     8881 ttggcagcta acttctggta acgcgctaag ataagatttg tcacctggtg gttgcaaaaa
     8941 gaacacagca tttcgaaatt ttttgcttct tctaatatct tttccacata gtttttagct
     9001 tttgtttggt ccgtatgcat taaatcttgc agattctcca tctcttggac aaattccagg
     9061 cgaagtgtat ttacttcttg gatatgctgt cgaagctgct cataatgttg ctccaacaaa
     9121 tcatttttga tttttaagaa atctacttgt ctccgatctt tgtcggtatt taagatgtga
     9181 tcgattagat tgtagctgat aataacgatt agaatgataa tatataaaat cgtagaccaa
     9241 ttgatcgttg tttgatagat acctaatgcc gtttggatgt aaatcaagca atggataatg
     9301 gctaccaaga cgatccaagg tgtacaagct acataaaaac ggttattctt atatgcttcg
     9361 ccaatgcatg caagtgaagt gactaaagtg cttataacac tagcaatatt agcaatcggc
     9421 attaattcag gaagttctgc aggagtaaaa gttgcataaa gcagaacgcc gataaaaatc
     9481 aaaagttgga acgtacaaaa aattccgtac cattttttat agtaaaacat tcggctccaa
     9541 tagtagcaga tgatgaaagc tgacgttaag ataataaata aatgagataa aaaagaatga
     9601 acgaatggtt caaaaagaat accacttaaa atatcttctg atacagcttc aaatcctaat
     9661 gccaaagcaa agcaacttaa gagaataagt gagtattcaa gacgtttttg tttatacatc
     9721 aagtagatcg tatagaaaaa ggtccctata gcgataaaaa cagcgattat catcgtcaat
     9781 acttgcggca ctgattgtga ataaataaaa ctaatcaatg gtccagttgt tcctagataa
     9841 actttcggtg gcagtccagc atagttctga taaggagatg agactgtaag ggtcaatttt
     9901 tttccgatat aatttgctgg taaatctatc acagccaatg agagtccggg attgctttct
     9961 tcctgataat ctttccgttg gtataaaatc ttttcgccta gatatacagt aatccattga
    10021 tggtttcctt tgattagtaa atttgcttcg tccaacggtt tttcaagtgt cgtttccata
    10081 atgaatgttt cgtctttatc cacggtagga atcgtacgag tgtacttgct gtcaaactta
    10141 aaatcaggat catcttttgt atagaaaacc cacgaatctt ttatttcttt catttgattg
    10201 ctgtccgcag gagaaaaaat aaaataaaag aaataagcaa ttc
//
LOCUS       DAZKLD010000106        10123 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.106, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000106 DAZKLD010000000
VERSION     DAZKLD010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10123)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10123)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10123
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.106"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(9..488)
                     /gene="rlmH"
                     /locus_tag="ACG8KR_002182"
     CDS             complement(9..488)
                     /gene="rlmH"
                     /locus_tag="ACG8KR_002182"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HFQ0201512.1"
                     /translation="MNIKIISVGKLKEKYLIQGINEYVKRLNAYAKIELIEVPDEKAP
                     ENLSEAQMRQVKEKEGERILAKIKEQEYVYALAIEGKNPTSESFAKQIDQLGIQGKSH
                     LVFVIGGSLGLSEAVMKRSNAQISFGKMTYPHQLMRLILVEQIYRAFRINAGAPYHK"
     gene            916..2205
                     /locus_tag="ACG8KR_002183"
     CDS             916..2205
                     /locus_tag="ACG8KR_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HFQ0201513.1"
                     /translation="MDRKNVTPKMKKSKNNSLWRKLGLGLVGGIVGGLLTAGIFYAAM
                     GTGNNSSTATSGNQNSAGETVVENVKVNVDSDITKAVDKVQDAVVSVINLQSQNQSSG
                     FGGLFGQQEESSSSDDSNLEAYSEGSGVIYKKDGNTAYVVTNNHVVDGQQGLEVLMKD
                     GTKVKAELVGTDAYSDLAVLKINSDKVETVASFGDSSALKVGEPAIAIGSPLGSEYAN
                     SVTSGIISSLNRQVTSTNESNETVNINAIQTDAAINPGNSGGPLVNIEGQVIGINSSK
                     IASTSESTSNVSVEGMGFAIPSNDVVNIINQLEKDGKVTRPALGITMVDLSAVSTQQQ
                     EQILKIPSSVTNGVIVTNVQTATPAEKAGLKQYDVITKIDDKEVSSGVELQSALYKKK
                     VGDSVKITYYEGSKEKTTTVELTIDQSALKQNQNSEN"
     gene            complement(2244..2849)
                     /gene="ytpR"
                     /locus_tag="ACG8KR_002184"
     CDS             complement(2244..2849)
                     /gene="ytpR"
                     /locus_tag="ACG8KR_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtpR family tRNA-binding protein"
                     /protein_id="HFQ0201514.1"
                     /translation="MIFAYNKAHVGDTLMVIIADDKGTENTVERKWNVARIKDEQGQV
                     VAWNFFHISDHLTIEGNGQVSLTDEELAKLNRLIKEAGFEEQLEADHEPKIVVGYVKT
                     CVPHPDSDHLSITETEVDNGTVLQIVCGAPNIEAGQKVVVAKPGAMMPDGMMIWPGTL
                     RGVDSYGMICSARELHLPNAPAKKGILVLPEDAVVGEAFPK"
     gene            complement(3054..3518)
                     /locus_tag="ACG8KR_002185"
     CDS             complement(3054..3518)
                     /locus_tag="ACG8KR_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFQ0201515.1"
                     /translation="MNQEYKNILVGIDGSEQALAAFKKAVEVARRNNGKVYVANVIDQ
                     QFYNFMGYAPIDQNLVDQQTETAKEMIEECKEYGKSVDYTNIEGIIAYGSVRESMAVK
                     LPKKYEIDLIMVGQSGLNAVERFMTGSVASYVIRHSPCDVLIVSDEEEQPSN"
     gene            complement(3540..3860)
                     /locus_tag="ACG8KR_002186"
     CDS             complement(3540..3860)
                     /locus_tag="ACG8KR_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HFQ0201516.1"
                     /translation="MIIPNSLEELAGYVETGKSVFFFTAGWCGDCRFIKPQMPEIEND
                     FSDWQFIEVDRDKYIDVAAEWNIFGIPSFVVIQDGKELGRLVNKDRKTKQEIESFLES
                     VTKG"
     gene            complement(4004..5083)
                     /gene="pepA"
                     /locus_tag="ACG8KR_002187"
     CDS             complement(4004..5083)
                     /gene="pepA"
                     /locus_tag="ACG8KR_002187"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HFQ0201517.1"
                     /translation="MEEKTFQRIKELTELQGTSGFEQDVRAYMESHMEPLVDELQLDG
                     LGGIFGLRHHEEADAPRVMVAAHMDEVGFMLTQIQENGLFKVVPLGGWNPYVVSAQRF
                     TLKTSTGKNYPCISSSVPPHLLRGTSGQKSVEVTDILFDAGFESREEAMSYGVLPGDT
                     IVPYAETIKTANGKNIISKSWDNRYGCTMVLEALEALQNETLGHTLIAGANVQEEVGL
                     RGSKASVNKFKPDLFFAVDCSAADDTVTKNGTFGHLGEGTLMRIQDPGLIMLPRLREY
                     LLDIAETNNIPYQYFVSKGGTDAGAAHTQNEGIPSTVIGVVGRYIHTHQTMFSIRDFE
                     AAREMLIQTLKGLDKSTVNTIVYGK"
     gene            5283..5633
                     /locus_tag="ACG8KR_002188"
     CDS             5283..5633
                     /locus_tag="ACG8KR_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HFQ0201518.1"
                     /translation="MYEESELSYFKGGLAVGIGLGLLSGVASTIWYHRKKVLSADQVL
                     ENIKSSFLKEGAIEGSWISFEKEPIRKFAVRSSAYRGGLSRIEDGETVYYEFLADAHT
                     GTVLEISRKKGATD"
     gene            5856..6803
                     /locus_tag="ACG8KR_002189"
     CDS             5856..6803
                     /locus_tag="ACG8KR_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFQ0201519.1"
                     /translation="MEQQEKIAVLIPCYNEEATISTVIADFKRELPEADIYVYDNNST
                     DRTYELAVQGGAIVKKEPRQGKGNVIRQMFFDIDADYYLMVDGDDTYPAEAVHGLLEK
                     LRSGEADMVIGDRLSNGTYFDENKRPFHDFGNNLVRNTITRMYKTKILDVMTGYRGFN
                     RIFVKSFPIMSSGFQIETELTIHALDKKFKFVEIPIDYRDRPEGSESKLNTFSDGFKV
                     IMMIIKMFKDYKPLLFFGIWTIFFFLFGLVTGIPVIREYMLTSFITRIPSAILSTGLM
                     TLALLSLVTGLILDTVVTNAKKEYELNLYHVYHEYHSRK"
     gene            complement(6860..8278)
                     /gene="pepV"
                     /locus_tag="ACG8KR_002190"
     CDS             complement(6860..8278)
                     /gene="pepV"
                     /locus_tag="ACG8KR_002190"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HFQ0201520.1"
                     /translation="MTIDWQKEVEARKEDLLEDLKNLLRVNSERDDSKVTPDAPFGPG
                     PRDALKHMLAYGERDGFKVKNVDNYAGHIDLGEGDETLGIFGHMDVVPAGDGWDTDPY
                     EPVIKDGKIFARGSSDDKGPSMAAYYAMKIIKELDLKLSKKVRFVVGSDEESGWGDMA
                     YYFEHEEEPDFGFSPDAEFPIINGEKGNVSLALRFKGDNAGDYVLKSFVSGLRENMVP
                     GTATAALQVPSADAAIAMEEAFYQFIEANPVSGTIEADNTYVKIELVGKGAHGASPQS
                     GINAGSFLALFLDNYEFLGSAKQFIHVAAAYVHEDFYGEKLGVAYEDEKMGKLTMNAG
                     LFAFEENGTEEANFINMNFRFPKGVTVDGLQSDIEQTVGQEGATVTRGARVMEPHYVP
                     MDDPLVATLLQVYEDHTGEKGYEQIIGGGTYGRLLKRGVAYGAMFPGYTDTMHQANEF
                     MSLDDLFRATAIYADAIYRLAK"
     gene            8427..9272
                     /locus_tag="ACG8KR_002191"
     CDS             8427..9272
                     /locus_tag="ACG8KR_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HFQ0201521.1"
                     /translation="MLINEQLVKRVITPRPKISHKGTFGRIVLIGGNEQYGGAIIMSA
                     LAAVHSGAGLTTVVTDEKNRSALHSHLPEAMIVVWTDTDQFLSVIKQADVLLIGPGLG
                     TSSKSLERFKQVLENQSKEQWLVIDGSALSLLAANHLLLPYPEQTVLTPHQMEWQRLS
                     SLPIQEQRDKENKKKQQEIGAVLVLKSHETKIYGHQHFYTNPLGSAAMATGGMGDTLA
                     GMITGFLAQFPQNDQTIAAAIYLHSFIGDQLAKEQYIVLPTQISEALPKWMNYFCKKS
                     MENEA"
     gene            9276..9716
                     /locus_tag="ACG8KR_002192"
     CDS             9276..9716
                     /locus_tag="ACG8KR_002192"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HFQ0201522.1"
                     /translation="MEKFTGYVREPFYYETDQMGIIHHSNYIRWFEEARVALLVHLGF
                     PYKKIEESGIIIPVLEVNCSYKEMIHYGEKVSIHPTIQKYTGTRLDFSYEIVGTEDKK
                     LKTTGSSKHCFLLSDSQRLVKLSKVNPELDQLFQAYASLDDVNS"
     gene            9766..9960
                     /locus_tag="ACG8KR_002193"
     CDS             9766..9960
                     /locus_tag="ACG8KR_002193"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0201523.1"
                     /translation="MKRKKQVIPMYVVKVMHGYIDKTGCRTREKNLDNLLIFKDKKES
                     EAFAKRIGGRVKPIQEVRPD"
BASE COUNT         3004 a         2007 c         1919 g         3193 t
ORIGIN      
        1 aaatatcatt atttgtggta tggagccccc gcattgatcc ggaaggcacg gtagatttgc
       61 tcgacaagga tcagacgcat caattgatgt ggatacgtca tttttccaaa agagatctga
      121 gcattgcttc ttttcatgac ggcttcgctg agtcctaaag agccgccgat aacaaaaaca
      181 aggtggcttt ttccttggat acctaattgg tcgatttgtt tagcaaaaga ttcacttgta
      241 ggatttttcc cttcaattgc taaggcatag acgtattctt gttctttgat tttcgctaag
      301 atccgttccc cttctttttc ttttacttgt ctcatttgcg cttcgcttaa attctccggg
      361 gctttttcgt caggaacttc gatcaattcg atttttgcat aggcattcag ccgtttgaca
      421 tactcattga taccttggat caggtatttt tcttttaatt ttcctactga gataattttg
      481 atattcatgt tttctctcct ttggtcttca tgctcacttt gtttattata atcaattttt
      541 tcgtctatat gtttattttg gctatttttg ttaaaaagat ttccccaaca gttatgcaca
      601 acatccacag gtttatccac agaaaacagt gttattttac cttatttcat cacattcatt
      661 aggcaaacat acgttttata agaatttatc cactttatac acaggctgtg gatatgtttt
      721 atcccttatt tttccaatcc tatccgcgta gttttacaca gtatctgtgg aaaaagaaaa
      781 tcaaatattt tcatctgaaa acaatgattt ttcctaaagt ttttcataaa aagttcacag
      841 tctcaaagta gactaaagtc aacaaataag actatactaa cagtagataa atgaatgtaa
      901 ttggaggagg aacacatgga tagaaaaaat gtgacaccaa agatgaaaaa aagtaaaaat
      961 aatagcctct ggcgtaagtt aggtctgggg cttgttggag ggatcgttgg aggactactg
     1021 acagctggca ttttttatgc agcgatgggc actggcaaca atagttctac tgctacaagc
     1081 ggcaatcaga attctgccgg tgaaacagtt gtggaaaatg tcaaagtcaa tgtagattct
     1141 gatatcacca aagcggtaga taaagttcaa gacgctgtcg tttctgttat caacttacaa
     1201 agtcaaaacc aatcaagcgg ttttggcgga ctattcgggc agcaagaaga aagtagcagc
     1261 tcagatgata gtaatttgga agcttatagt gaaggaagcg gcgtgatcta caaaaaagac
     1321 gggaatactg cttatgtagt cacgaataat cacgtagtag atggccagca aggtcttgaa
     1381 gtcttgatga aagacggtac gaaggtaaaa gctgaattag ttggaacaga tgcttattca
     1441 gatttagcgg tgttgaagat caattcagac aaagtcgaaa cagttgcctc ttttggtgac
     1501 tcaagtgctc tgaaggttgg tgagcctgcg attgcgattg gttccccact aggatctgaa
     1561 tatgcaaatt ctgtcacttc aggaatcatt tcttccttga accgacaagt gacaagtacg
     1621 aatgaatcga acgaaacagt caatatcaat gcgatccaaa cagatgcagc aatcaaccct
     1681 ggaaattctg gtggtccttt agttaatatc gaaggacaag ttatcggtat caactcaagt
     1741 aagatcgcaa gtacttctga atctacttct aatgtaagcg tagaaggaat gggatttgct
     1801 attccaagta acgatgtagt gaatatcatc aatcaactgg aaaaagacgg aaaagtgaca
     1861 cgtccagctc taggtatcac aatggtcgac ttatctgccg tttctactca gcaacaagaa
     1921 caaatcttga agattccttc atctgtaact aacggtgtca tcgtgacaaa tgtccaaaca
     1981 gctacaccgg ctgaaaaagc tggattgaaa caatacgacg tcattactaa gatcgatgac
     2041 aaagaagttt cttctggtgt agaacttcaa tctgctttgt ataagaaaaa agtgggagat
     2101 tctgtcaaaa tcacttacta tgaaggctcg aaagaaaaaa caactactgt tgaattgacg
     2161 atcgatcaat cggcattaaa acaaaatcaa aatagtgaaa attaataaaa aagaggttgt
     2221 gacaaagact tgtcacaacc tctttatttt ggaaatgctt ctccgactac ggcatcctca
     2281 ggcaatacta agattccttt ttttgctgga gcatttggca aatgcaattc acgagcggaa
     2341 cagatcattc catagctatc tacccctcgc aaagttcctg gccagatcat cattccatct
     2401 ggcatcatag ctcctggttt tgcaacgaca actttttgtc cagcttcgat attgggtgca
     2461 ccgcaaacga tttgcaatac tgtaccattg tctacttctg tctcagtaat cgataaatgg
     2521 tcagaatcag gatgtgggac gcatgttttt acgtacccga caacgatctt cggttcatga
     2581 tcagcttcaa gctgttcttc gaatcctgct tctttgatca atcgattcaa tttcgctaat
     2641 tcctcatctg ttaagcttac ttggccgttc ccttcgattg ttagatgatc ggagatatgg
     2701 aaaaagttcc aagcaacaac ttgaccttgt tcatctttga tccgagcaac attccatttg
     2761 cgttcaacag tgttttctgt tcctttatcg tcggctataa taaccatcaa ggtgtctccg
     2821 acgtgtgctt tgttataagc aaaaatcatg ttctttcctc cattatcctt tgctgagatt
     2881 tatcttaaca aaaaattgca ttcactgcat gaaatttttt tcatgcttta tttccgaaga
     2941 agcctcttct ggaacaccat ttattcgtaa caagggactg tgacaagact tttgtcacag
     3001 tccctttact gttttagtta attgattaaa aaggtcgtac aagttccact tgcttaatta
     3061 cttggctgtt cttcttcgtc agaaacgatc aagacatcac aaggtgaatg acgtatcacg
     3121 tagctggcta cacttcctgt cataaagcgt tcgactgcat ttagacctga ctgcccaacc
     3181 atgattagat cgatctcata tttttttggc aatttgactg ccatggattc acgaacagaa
     3241 ccatatgcaa taattccttc tatatttgtg taatcaacag atttcccata ttctttacat
     3301 tcttcgatca tttccttggc tgtttcagtt tgctgatcga ctagattttg atcaataggt
     3361 gcatagccca taaagttata aaattgctga tcgattacgt ttgctacata tactttccca
     3421 ttattacggc gagccacttc taccgctttt ttgaaagcag caagtgcttg ttcactaccg
     3481 tcgataccta ctaaaatatt cttatattct tgattcatct tgacccctcc taacagatgt
     3541 cagccttttg ttacactttc taggaacgat tcgatttcct gttttgtttt acgatcttta
     3601 ttcactagac gacctaattc ttttccgtct tggatcacga caaaacttgg gataccaaaa
     3661 atgttccatt cagcggctac atctatgtac ttgtcacggt ctacttcaat aaattgccaa
     3721 tcagaaaagt cattttcaat ttcaggcatt tgcggtttga taaaacggca gtctccgcac
     3781 cagccggcag taaagaaaaa tacactcttt ccagtttcta cgtagcctgc taattcttct
     3841 aaactatttg ggataatcat tctttgtcct ctactttctg tttatgtatt tttcaattaa
     3901 attatacacc tttctttcga actaacaaac attgatcctc tttttataaa aaaaatctgg
     3961 caaaaagcaa tcttttgcca gattttatag tttcactatc gcattatttt ccgtatacga
     4021 tcgtatttac tgttgattta tccaaacctt tcagtgtttg aatcaacatt tctcgtgcag
     4081 cttcaaaatc gcgaatactg aacatcgttt gatgcgtatg gatataacgt cccactacgc
     4141 caatcactgt acttgggatt ccttcgtttt gtgtatgagc agcacctgca tctgtaccgc
     4201 cttttgaaac aaagtattga taagggatat tgtttgtttc agcgatatcc agcaagtact
     4261 cgcgcaaacg aggaagcatg atcaaacctg gatcttggat acgcatcaat gtaccttctc
     4321 ctaaatgtcc gaatgtgcca tttttcgtca ctgtatcatc tgctgcagag cagtctactg
     4381 caaagaacag atctggcttg aatttattga cagaagcttt agaaccacgc aagcctactt
     4441 cttcctggac attcgcgcct gcaatcaatg tatgacctaa tgtttcattt tgcaacgctt
     4501 ctaaagcttc taataccatt gtacagccat agcggttgtc ccatgattta ctgataatat
     4561 tctttccgtt ggctgttttg atcgtttcag cgtaaggaac gattgtgtct cctggaagga
     4621 caccgtaact cattgcctct tctcttgatt caaacccagc atcgaacaaa atatcggtta
     4681 cttctactga tttttgtccg cttgttcctc gcaataaatg tgggggaacg gaagaagaaa
     4741 tacatggata attcttacca gtagaggttt taagtgtaaa acgctgagca gaaacaacgt
     4801 aaggattcca gccgcctaga ggtacgactt taaaaagccc gttttcttgg atttgtgtca
     4861 gcatgaatcc gacttcatcc atatgtgctg cgaccattac acggggtgca tccgcttctt
     4921 catgatggcg cagaccaaaa atgccgccca aaccatctaa ctgtaactca tctaccagcg
     4981 gttccatatg actttccata taagcgcgaa catcttgttc aaaaccgctt gttccttgta
     5041 attctgttaa ctctttgatt cgttgaaatg ttttttcttc catatgctat tcgtctaaat
     5101 ttcagactaa actcctctca gacttatatt gatctgcgga taaaatatga ttttttctgc
     5161 agaccgatcg tatttattat agcttatttt ttctggtagc ggttatttat ttcaaagaaa
     5221 atatgagagt gtggtaaaat aagcacaagg agtaacaagt gagaagaagg aggtatgtcc
     5281 acatgtatga agaaagcgaa ctaagctatt ttaaaggcgg gttagcagtt ggaatcggtc
     5341 taggattatt gagcggggta gcttcaacga tttggtatca tcgcaaaaaa gtattgtcag
     5401 cagaccaagt acttgaaaat atcaagtcat cttttctaaa agaaggtgca atcgaaggtt
     5461 cttggatctc ttttgaaaag gaacctatta ggaaattcgc cgttcggtcg agtgcttatc
     5521 ggggaggtct ttctcgaata gaagatggcg aaactgttta ttatgaattt ttagcagatg
     5581 ctcatacagg aaccgtatta gaaatttcaa gaaaaaaagg tgcaacagat taatacgtca
     5641 aaaaagtgag agaacgaata cgcacccact attcaaaaga gaggtatcat gtccttcttt
     5701 tgtagtgggt ggttttttag atagagcttt ccaaacatat attccagtat tttcttttta
     5761 aataaatgat atttagcggg aaaaactagc atttcctttg attatgaggt atttttaatt
     5821 tagcttttgt tttctatagt taaggagagt gaaaaatgga acaacaagaa aaaattgctg
     5881 ttctgattcc ttgttacaat gaggaagcaa caatttcaac agtcattgca gactttaaac
     5941 gagaattacc agaagcagat atttatgtct atgataataa ttcgaccgat cgaacatacg
     6001 aattggccgt tcaaggtgga gcaatcgtaa aaaaagaacc tcgtcaagga aaaggaaatg
     6061 tgatccgcca aatgttcttt gatatcgatg ccgactatta tttaatggta gatggcgatg
     6121 atacttatcc tgcagaagct gtacatggtt tgctcgagaa attacgttct ggtgaagcag
     6181 atatggtcat tggtgatcgc ctatcaaatg gaacctactt tgatgagaat aagagacctt
     6241 tccacgattt tggaaataac cttgttagaa atacaattac tagaatgtat aaaacaaaaa
     6301 ttcttgatgt catgaccggc tatcgaggat tcaatcgtat ctttgtcaaa agttttccta
     6361 ttatgagttc tggttttcaa atcgaaacgg aattgacgat ccatgcttta gataaaaaat
     6421 tcaagttcgt tgaaatccca atcgattatc gtgatcgtcc tgaaggcagc gaatcaaaat
     6481 tgaatacatt ctctgacgga ttcaaagtga tcatgatgat tattaaaatg tttaaagatt
     6541 ataagccttt gcttttcttt gggatttgga ccatcttctt ctttttattt ggattggtca
     6601 cggggattcc tgtgattcgc gaatatatgc tgacaagctt tattacacgc atcccttctg
     6661 cgattttaag tactggattg atgactttag cacttctttc attagtaaca gggttgattt
     6721 tggatacagt cgtcacgaac gctaagaaag aatatgaatt gaatctatat catgtttatc
     6781 atgaatacca cagtagaaag taaaaaaagg atcaagactg caaaaataat ttgcaattct
     6841 tgatcctctt tttaattaat tatttcgcca aacgatagat ggcatctgca tagattgctg
     6901 ttgctctgaa caagtcatca agactcataa attcgtttgc ttgatgcatt gtatcagtat
     6961 atcctgggaa catcgctcca taagcaacgc cacgtttcaa caaacgtccg taagttccgc
     7021 caccgatgat ttgttcgtag cctttttctc cagtgtggtc ttcatagact tgcaataatg
     7081 tagccaccaa tggatcatcc attggtacgt aatgtggttc cattacgcga gcacctcgag
     7141 taactgttgc gccttcttgc cccactgttt gttcgatgtc actttgcaat ccgtctactg
     7201 ttacaccttt tggaaaacgg aaattcatat tgatgaagtt cgcttcctct gtaccatttt
     7261 cttcaaaagc aaatagtcca gcattcattg tcaatttgcc cattttttca tcttcataag
     7321 cgactcctag tttttctcca taaaaatctt catggacata agcagctgct acatggataa
     7381 attgtttagc agatcctagg aattcatagt tatctaagaa caatgccaag aaagaacctg
     7441 cattgatacc agattgtggg cttgcaccat gtgcgccttt accaactaat tcgattttta
     7501 catacgtatt gtccgcttca atcgttccgc ttactggatt tgcttcgatg aattggtaaa
     7561 atgcttcttc catcgcgata gctgcatctg cacttggtac ttgaagtgct gcagttgctg
     7621 ttcctggcac catattttcg cgaagtccgg aaacaaaaga tttcaagaca taatctcctg
     7681 cattgtcgcc tttgaagcgt aaagctaaag aaacatttcc tttttcacca ttgatgattg
     7741 ggaattcagc atctggtgag aaaccaaaat ctggttcttc ttcatgttcg aaataataag
     7801 ccatatcgcc ccagccgctt tcttcatcgc tgccgactac gaagcgaact ttttttgata
     7861 gtttcaagtc aagttcttta atgatcttca tcgcatagta agctgccata cttggacctt
     7921 tgtcatcgct tgatccgcgt gcaaagatct ttccatcctt gattactggt tcataaggat
     7981 ctgtgtccca gccatctcct gcaggaacga catccatgtg tccaaatatc cctagcgttt
     8041 cgtctccttc gccaagatca atatgccctg catagttatc gacatttttt actttaaatc
     8101 catcacgttc gccataagct aacatatgtt tcaatgcgtc gcgaggtcca ggtccaaatg
     8161 gtgcatctgg tgttactttt gagtcatcgc gctcgctgtt gacacgtaaa agatttttca
     8221 agtcttcaag caagtcttct ttacgtgctt ctacttcttt ttgccaatca attgtcataa
     8281 agttaatcct ccacttattt aagtcttcgt gttcatgata ccacaaatta cagaaaaaaa
     8341 tagttcaaaa aatctgacaa ttccttttct tatctgtttt tctctaaaaa aaatatacgc
     8401 tataatgagt aaaaggaggt ttttagatgc tgataaacga acaactagtg aaaagagtca
     8461 tcacacctcg tccaaaaatt tcacataaag gaacatttgg acgtatcgtt ttgattggcg
     8521 gcaatgaaca atatggcgga gcaatcatta tgagtgcttt agctgccgta cacagcggag
     8581 cgggtctcac gactgtagta actgatgaaa aaaatcggag tgctcttcac agtcatctgc
     8641 cagaagcaat gattgtagtt tggacggata ctgaccagtt tctttccgta ataaagcaag
     8701 cagatgtact cttgattggt cccggattgg gtacttcttc taagagtttg gaacgtttca
     8761 aacaagtact agaaaaccaa tcaaaagaac aatggctggt gattgatggc tctgctttaa
     8821 gtttacttgc tgctaatcat cttttgctac cctatcccga acaaactgta cttacacctc
     8881 atcaaatgga gtggcaacgg ttgagttctc taccgataca agaacaacgg gataaagaaa
     8941 acaaaaagaa gcaacaggaa atcggtgctg tgctcgtttt gaaaagtcat gagacaaaaa
     9001 tttatggtca ccagcacttc tacaccaatc ctttaggttc tgctgctatg gcaacgggcg
     9061 gcatgggtga cactttagct gggatgatca caggattttt agcccagttt cctcaaaatg
     9121 accaaacgat tgcagcagct atttatcttc atagtttcat tggagaccag ctggcaaaag
     9181 aacaatatat tgttttgccg acacaaatca gcgaagcact tccgaaatgg atgaattatt
     9241 tttgtaaaaa atcaatggaa aacgaggcat gagtcatgga gaaatttaca ggttatgtgc
     9301 gagaaccttt ttattatgaa acagatcaaa tggggattat tcatcacagc aattatatcc
     9361 gctggtttga agaggctcgg gtagccttat tggtacatct agggtttccc tataaaaaaa
     9421 tcgaagaatc tgggatcatc atccccgttc tggaagtaaa ttgcagctat aaagaaatga
     9481 tccattacgg agaaaaagtg agcatccatc caactatcca aaagtatacg gggacacggt
     9541 tggattttag ctacgaaatt gtgggaactg aagacaaaaa attaaaaact actggcagta
     9601 gtaaacactg ttttttactt tctgattcgc aacgattagt gaagctgagc aaagtcaatc
     9661 cggagttaga tcagttattc caagcgtacg cttctttgga tgatgtaaat tcttaataaa
     9721 gaccgtctaa gcatttttct tgtattgtgt cgagtagttc gtataatgaa aagaaaaaag
     9781 caggtgatcc ccatgtatgt cgttaaagta atgcacggtt acatcgataa aactggctgt
     9841 cgcactcggg aaaaaaactt agacaatctg ctcattttca aagacaaaaa agaatccgaa
     9901 gcttttgcta aaagaatcgg cggtcgtgtc aaaccgatcc aagaggtacg accagattga
     9961 ttcaatattt ttcttttcga ggctctaata ggcatgccta tcagggcttt ttttgcagac
    10021 tgtgttttta tgcccacacg atttgtaagc gatttatgtt ataatagatt atatctgcat
    10081 agaaaggaag gttaaatggt tatcaataat ttgcaacagc aag
//
LOCUS       DAZKLD010000107        10025 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.107, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000107 DAZKLD010000000
VERSION     DAZKLD010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10025)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10025)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10025
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.107"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            171..440
                     /gene="rpsN"
                     /locus_tag="ACG8KR_002194"
     CDS             171..440
                     /gene="rpsN"
                     /locus_tag="ACG8KR_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HFQ0201524.1"
                     /translation="MAKKSKIAKAKKQMAMIEKYADKRQELKAAGDRTALAKLPRDSN
                     PNRLRLRDQTDGRPRGYMRKFGMSRIKFRELAHQGLIPGVKKASW"
     gene            455..634
                     /gene="rpmF"
                     /locus_tag="ACG8KR_002195"
     CDS             455..634
                     /gene="rpmF"
                     /locus_tag="ACG8KR_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018345355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HFQ0201525.1"
                     /translation="MAVPARKTSKAKKRLRRTHQTVVKPEISFDEANGDYRRSHHVSL
                     KGYYNGKKVIKGASK"
     gene            666..1043
                     /locus_tag="ACG8KR_002196"
     CDS             666..1043
                     /locus_tag="ACG8KR_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0201526.1"
                     /translation="MEIIYPPLVEEGLKYYLETTQQSLDKCTFYRSMVERGIITETGL
                     PTQQAIENGLVKDYYEDQGLSFDDFLRIYPIFEEYDEELFQCIDGYWEIPVDMKENLV
                     SQLESGELTFEDAQQIQAYLEDR"
     gene            1064..1213
                     /gene="rpmG"
                     /locus_tag="ACG8KR_002197"
     CDS             1064..1213
                     /gene="rpmG"
                     /locus_tag="ACG8KR_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFQ0201527.1"
                     /translation="MRQNIILECVETGERLYLTSKNKRNNPERLELKKYSPKLRRRAI
                     FKEVK"
     gene            1237..2220
                     /locus_tag="ACG8KR_002198"
     CDS             1237..2220
                     /locus_tag="ACG8KR_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFQ0201528.1"
                     /translation="MGIPVTIVSGFLGAGKTTLINQALQVSPYPREEIIIIENEFGEV
                     GIDHKLLVHSEERVLQLNNGCMCCSLRSDLLAILISLLETIEEHQQPISQIIIETTGI
                     ADPQPIIQTLLTAPQLRGRVYIDSLLTVIDSDSFELIEREPQALKQLVMADRIFVSRK
                     QNLNLSALKKLQREIKSINPLSDIRMFTHSETIQKKDFYNLKKFNHPLVFESDSSENH
                     YNGKYYHQHHQVHEFKSILLTSDQDIKKDFLLQWIDWLILTNQGSLYRFKGLIALQEQ
                     DFIVAMQEVNEQVNFQYTTRQKDQTTIILIGKMLDEERIKQSFDKLLAESK"
     gene            2250..2372
                     /locus_tag="ACG8KR_002199"
     CDS             2250..2372
                     /locus_tag="ACG8KR_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HFQ0201529.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKEHKRNKMKKLA"
     gene            2390..3913
                     /locus_tag="ACG8KR_002200"
     CDS             2390..3913
                     /locus_tag="ACG8KR_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HFQ0201530.1"
                     /translation="MKKISVGLIGIAALGLLGACSSTNDAKVSNDKDGKLEIVTTFYP
                     MYDFTKNIVGDEANVDLMVPAGSEPHDYEPSAKDMAKAHDADVFVYHNENMESWVPKA
                     KESWKKAGPNVVEGTKDMILLPGSEEEDHDHGEEDHHHELDPHTWVSPKMAIKEVSNI
                     KDQLVKLYPKKAKVFETNAEKYLTKLKRLDADYTTSLKEAKQKSFVTQHAAFGYLALD
                     YGLKQVPIAGLSPEEEPSSGRLAELKEYVKKNKINYIYFEKNANDKIAKTLANEAGIK
                     LEVLNPLESLTKEQMDNGEDYVSVMEDNLKALEKTTMVAGEEVVPEKEAKDEKTVANG
                     YFKDADVKDRELSDYTGEWQSVYPLLKDGILDEVFDYKAKINKDMTAAEYKDYYTTGY
                     KTDIDTINIKDNTIDFVVNGEHHQYTYKYVGYKILNYEKGNRGVRFNFETDDAGAGRF
                     KYIQFSDHGIAPSKAEHFHIFFGGESQEKLYNELHNWPTFYPASLSKHEIAQEMMAH"
     gene            3937..4176
                     /locus_tag="ACG8KR_002201"
     CDS             3937..4176
                     /locus_tag="ACG8KR_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0201531.1"
                     /translation="MVRKVLLSMDSKNIDAYRRYRFAKQMRLNGSTLADIAATLHVSI
                     DTVHRVLKRDPDCCFIKVNTGRSRRLAKRHNSLAN"
     gene            complement(4369..4830)
                     /locus_tag="ACG8KR_002202"
     CDS             complement(4369..4830)
                     /locus_tag="ACG8KR_002202"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFQ0201532.1"
                     /translation="MHHRGKTRHTKNHVESRGKICLTHSIHGRPKSADDRSDFGHWKA
                     DIVAGKTGGTCLVTLTDRKSCFLLAGKKVPRKKAAYVSEKMIELLSSIPNEAVKTITP
                     DRGKEFSNHFAVTEALNNMPFYFPDPHALGNNEQIKIRIDSFENTCQNRKR"
     gene            complement(5025..5243)
                     /locus_tag="ACG8KR_002203"
     CDS             complement(5025..5243)
                     /locus_tag="ACG8KR_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0201533.1"
                     /translation="MSQYRHLTILERERIFLLHEEGFSISIIGNDIQHSPFTILHELR
                     RNKKKTIHLPKRKKIMLIEEKNADVITF"
     gene            5361..6977
                     /locus_tag="ACG8KR_002204"
     CDS             5361..6977
                     /locus_tag="ACG8KR_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFQ0201534.1"
                     /translation="MKKIPITILTGYLGSGKTILLNHILYGDHGKEIAVIVNDIGEVN
                     VDGVLVDKKGFKRTGEKLVSMPNGCICCTLREDLIVELDQLVQFSDLNQIVIEASGIS
                     KPIPIIQAIILAKNPLDIDLTQRLEIGAIIIVVDVKRFWDNFETGEKIQERKAEGNVV
                     EEEQDIRGLLIDQFECCNILLLNNSDLVSTKTIYKINGLIAKLQSNAKIIRTIKGEVD
                     LSKILNTNLFNIDETSYSVGWFKELELGYQNHIPETEEYGISSFIYRNRLLLDAQKFA
                     HLTDKNFPGNITRAKGFLWFAQHSDFSTIFETSGSYKSIESLNYWVASLPKIECQMII
                     QKNPELKEAWHPLFGERINKLVFIGMKMDQQCIKEQLEGCLTDSLNADNFRKISPFPW
                     KKSNMFSQISLMKFLRKAGVKIGDVIIIGILIITSFIPLFVFSIEKENETTEDAIKYA
                     IVRIDGKEMDRFDLDKINHKLVNYHPAKGQYNIVEIKDGRIRVKEDNSPDQIAVRTGW
                     ISKPGQTSICLPHKLVISIEKKESTDYYIY"
     gene            complement(7218..8558)
                     /locus_tag="ACG8KR_002205"
                     /pseudo
     CDS             complement(7218..8558)
                     /locus_tag="ACG8KR_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(8996..9142)
                     /locus_tag="ACG8KR_002206"
     CDS             complement(8996..9142)
                     /locus_tag="ACG8KR_002206"
                     /inference="COORDINATES: protein motif:HMM:NF012870.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0201535.1"
                     /translation="MMDITYIPCNDGRLYLSTYIDLATRILRYYMVDSHMKKEIVVRP
                     LQIY"
     gene            complement(9181..9375)
                     /locus_tag="ACG8KR_002207"
     CDS             complement(9181..9375)
                     /locus_tag="ACG8KR_002207"
                     /inference="COORDINATES: protein motif:HMM:NF024673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFQ0201536.1"
                     /translation="MFYEHKDRYGSPGITQKLYDEGIEINKRVVAVLMREMNLCTRGF
                     HRRKSSHGREKTIKEMVKKC"
     gene            complement(9285..9617)
                     /locus_tag="ACG8KR_002208"
     CDS             complement(9285..9617)
                     /locus_tag="ACG8KR_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFQ0201537.1"
                     /translation="MRKTYDRKFKLKISQDILEKKVTTKKIAEEYNISRPTISRWVSE
                     YRRYGKNAFAGQGKRLPDKADFYILEQENKRLKEEDECSTNTKIDTDLQGSLKNFTMK
                     ESKSINVL"
BASE COUNT         3334 a         1595 c         1985 g         3111 t
ORIGIN      
        1 atctctatgt accgataaat tcgttttcaa atagttatct attcatatga gttttatttc
       61 atgttattta ttgttgacaa gagcaactct tgttggtaaa gtatcagtgt caaaacgtaa
      121 cgattacgat ttgaggtgtc taaatcaaaa taaaaggagt ataaaggtat atggcaaaaa
      181 agtcgaaaat tgctaaagca aagaaacaaa tggcgatgat tgaaaagtat gcagataaac
      241 gtcaggaatt aaaagccgca ggagatcgca ccgctctcgc taaattgcca agagattcaa
      301 atccgaatcg tctgaggctt cgtgatcaga cggatggtcg tccaagaggg tatatgcgaa
      361 agttcggtat gtcccgtatt aagtttagag aacttgccca tcaagggttg attcctggag
      421 taaaaaaagc aagttggtaa aaggaggaaa tatcatggca gtaccagcaa gaaaaacatc
      481 taaggcgaag aaacggttaa gaagaactca tcaaactgta gtaaaaccag aaatttcatt
      541 tgacgaagcg aatggtgatt atagacgtag ccatcatgta tctttaaaag ggtactataa
      601 tggaaagaaa gtaattaaag gagcttcaaa atagtttaat atataacttt taggagggaa
      661 tcaaaatgga aattatttac ccacctttag ttgaagaagg attaaagtat tatcttgaaa
      721 cgacccagca atcgctagat aaatgcactt tttatcgttc gatggtagag agaggaatta
      781 ttactgaaac gggtttaccg acccaacagg caattgaaaa tggattagtc aaagattact
      841 atgaggacca aggcttatca tttgatgatt ttttaagaat ctatccaatt tttgaagagt
      901 atgacgagga gttatttcaa tgtatcgatg gttattggga gatacctgtt gatatgaaag
      961 aaaatttagt ttcacaattg gaatctggcg agttaacttt tgaagatgca caacaaattc
     1021 aagcatatct tgaagatcga taagtattga aggaggaaga tttatgcgtc aaaatattat
     1081 tttggaatgt gtcgaaactg gagaacgtct gtatctcacc agcaaaaata aacggaataa
     1141 ccctgaacgg ttagaattaa aaaagtattc gccaaaattg cgcagacggg caatttttaa
     1201 agaagtgaaa tagctaagtg gtaaaaaggt gacaaaatgg gcataccagt tacaatcgtt
     1261 tctgggtttt taggggctgg aaaaacaacg ctaatcaatc aagccttgca agtctcaccc
     1321 taccccaggg aagaaattat tataattgaa aatgagtttg gtgaagttgg gattgatcac
     1381 aaattattag ttcatagcga ggaacgggtg ttacaactaa ataatggatg tatgtgttgc
     1441 agtcttcgca gtgatttatt agcaatattg atttctttac tggaaacaat agaagaacat
     1501 caacaaccaa tttcacaaat catcattgaa actacaggta tagcagatcc ccaaccaatc
     1561 attcaaacac ttttaacagc gcctcaacta agaggaagag tatatatcga tagtttattg
     1621 actgttattg atagtgacag ttttgaactt attgaaagag aaccacaagc ccttaagcaa
     1681 cttgtcatgg cagatcgcat ttttgtgtct agaaaacaaa acttgaattt atctgcacta
     1741 aaaaaattac agagagaaat caaaagtatt aaccctttat ccgatattcg aatgttcaca
     1801 catagtgaaa cgattcaaaa aaaggatttt tataatttga aaaaatttaa tcatccctta
     1861 gtttttgaaa gtgattcatc agaaaatcac tataatggca aatactatca tcaacaccat
     1921 caagtacatg agtttaaatc aattctctta acttcagatc aagacataaa aaaagatttt
     1981 ttactccaat ggattgactg gctgatttta accaaccaag ggagcctgta tcgttttaaa
     2041 ggattgattg ctttacaaga gcaggatttt atagttgcca tgcaagaggt aaacgaacag
     2101 gttaattttc aatacaccac tcgacaaaaa gaccaaacca ctattattct aatcggaaaa
     2161 atgttggatg aagaaaggat caaacagagt tttgataaat tactggcgga aagcaaataa
     2221 tgataagtag tgattggggg taatttctaa tggagaaaac ggatatatct agtgcatatc
     2281 gacgacttaa aagcccgaat attaaaacta gaaaaagggc attaaaaatt atcaaagaac
     2341 ataaacgaaa taaaatgaag aaattagcat aattaaggaa ggaaaaagaa tgaagaaaat
     2401 cagtgtagga cttattggga tagcagctct aggattattg ggagcttgct ccagcactaa
     2461 tgatgcaaaa gtatcgaatg ataaagatgg aaaattagaa attgtaacaa cattctatcc
     2521 aatgtatgat ttcaccaaaa acatcgtcgg cgatgaagcg aacgtggatt taatggttcc
     2581 agcgggatca gaacctcatg actatgagcc ctcagctaag gatatggcaa aagctcatga
     2641 tgctgatgtt tttgtatatc ataacgaaaa catggaatca tgggtaccaa aagctaaaga
     2701 aagttggaag aaagctgggc ctaatgtagt agaaggaaca aaagacatga ttttattgcc
     2761 aggtagtgaa gaagaagatc atgatcacgg agaagaagat caccatcatg aactagatcc
     2821 tcatacgtgg gtttcaccca aaatggcaat taaggaagtt tcaaatatca aggatcagtt
     2881 agttaaattg tatccgaaaa aagcaaaagt atttgagaca aatgctgaaa agtatttgac
     2941 taaactgaaa cggttggatg ctgactatac cactagtctt aaagaagcga aacaaaaaag
     3001 ttttgtgacg caacacgcag cctttggata tttagctttg gattatggat tgaaacaagt
     3061 tcctattgct ggactaagtc ctgaagaaga gccatcttct ggtcgattag ctgaattaaa
     3121 agagtatgtt aaaaaaaata agatcaatta tatttacttt gaaaagaatg ctaatgataa
     3181 aattgcaaaa actttggcca acgaagctgg gataaaattg gaagtactga atcctcttga
     3241 aagtcttaca aaagaacaaa tggataatgg tgaagattat gtttcagtaa tggaagacaa
     3301 tctaaaagcg ttagaaaaaa caactatggt tgctggtgaa gaggtagttc cagaaaaaga
     3361 agcgaaggat gaaaagacag ttgctaatgg gtactttaaa gatgccgatg tgaaggatcg
     3421 cgaattgagt gattatacag gcgaatggca atctgtttat ccgttgttaa aagatggaat
     3481 tttggatgaa gttttcgatt acaaagcaaa aatcaataaa gacatgacag ctgccgagta
     3541 caaagattat tatactactg gctataagac agatattgat actatcaaca ttaaagacaa
     3601 caccattgat tttgtagtca acggtgaaca tcatcaatac acatacaaat atgttggata
     3661 caaaattttg aattatgaaa aaggaaaccg tggtgttcgt tttaattttg aaactgatga
     3721 tgcaggtgct ggtcgtttta aatatattca atttagtgat catggaattg caccaagtaa
     3781 ggcagagcat ttccatattt tctttggtgg agaaagtcaa gaaaaactct ataatgaatt
     3841 gcataattgg cctactttct atccagcttc attgtctaaa catgaaattg ctcaagaaat
     3901 gatggctcat taaaattcaa tttttggagg aaatacatgg taagaaaagt attattgtct
     3961 atggattcca agaatattga tgcttatcgg cgttatcgat ttgcaaagca aatgagatta
     4021 aatgggagta cattagctga tattgctgca actttacatg ttagtattga cacagttcat
     4081 agagtattaa aacgggatcc tgattgttgt tttatcaagg tcaatactgg aagaagtaga
     4141 cgtttagcaa aacggcataa tagcttggcc aattaattga taataccaga gtagatcatt
     4201 tttcccttat ttggatttga tctgcttttt attatgtctt gaattgtcaa atcaagcgca
     4261 acactttatc aaagaagact tcataaggtg ttctccaatc taaacacttt ctaggtctgt
     4321 tattcagttt ttcaatcatc tgatttatcg ttgagttatc taccgtagct atctctttcg
     4381 attttggcaa gtattctcga atgagtctat tcgtatttta atttgttcat tgttgccaag
     4441 ggcatgagga tctggaaaat aaaagggcat attattcaat gcttctgtaa ctgcgaaatg
     4501 attggagaat tcttttcctc tatctggagt gatcgttttg accgcctcat ttggaatact
     4561 gcttaaaagt tcaatcattt tctcgctcac ataagcggct ttctttctgg ggaccttctt
     4621 tcctgctaaa aggaagcatg attttctgtc tgtcaaagtc actaaacaag taccaccagt
     4681 tttgccagct acaatatcgg ctttccaatg accaaaatca cttctatcat ctgcggattt
     4741 tggacggcca tggatcgaat gagtaaggca aatctttcca cgagactcga catgattttt
     4801 tgtatgtctt gtttttcctc gatggtgcaa tgaacggatc aggcctttat tgccgtgtga
     4861 gaggggttct gtatcgaaaa gatcattgca aattgcacgt tagatcgtga taaaactaat
     4921 tgaaaagtcc atatgttcat gcttcaaacg attcgagatt tcctctggcg accactgaaa
     4981 atcgacaaat aaatgacgaa caattgacca aactcacgga tcatttagaa tgtgataacg
     5041 tccgcatttt tttcttcgat aagcataatt tttttccgct ttggaaggtg aatagttttt
     5101 ttcttgtttc ttctcagttc atgtaaaata gtaaacgggc tatgctgaat gtcgttgcca
     5161 attatactaa tagaaaatcc ttcttcgtga agcagaaaga ttctttctcg ttcgagtata
     5221 gtaagatgac gatactggct cataattatt ttccttctag gagtgttctc gacaaactta
     5281 tactagttgg atatcgatta tgactcattt ttatgtgttg cacttaaagt gtaaattcaa
     5341 ggtatagaaa gaggttccct atgaaaaaaa tacctattac aatcttaact ggctatcttg
     5401 gttcaggaaa aacgatactt ctaaatcata ttctgtacgg tgatcacgga aaggaaattg
     5461 ctgtcattgt aaatgacata ggggaagtaa atgttgatgg ggtattagtc gacaagaaag
     5521 gttttaaaag aactggagaa aaacttgttt cgatgccgaa tggatgcatt tgttgtacat
     5581 tacgcgaaga tttgattgtt gaactagatc aattagtgca attttctgac ttgaatcaaa
     5641 ttgttattga agcttcagga atcagcaaac caattccaat tattcaagca attatattag
     5701 ctaaaaaccc tttagacatt gatttgaccc aacgattaga gattggtgcc attattatag
     5761 tagtggatgt aaaacgtttt tgggataatt tcgaaacggg tgaaaagatt caggaaagaa
     5821 aagctgaagg taatgtagtt gaagaagaac aggatattcg tggcttgtta attgatcaat
     5881 tcgagtgttg taacatatta cttttaaata atagtgactt agtgtcaacc aagacgattt
     5941 acaaaattaa cggtctaatt gctaagttac agtccaatgc caaaataatt aggacaatta
     6001 aaggtgaagt agacttatcg aaaattctaa atacaaatct ttttaatata gatgagactt
     6061 cctattcagt gggctggttc aaagagttag agcttggcta tcaaaatcat attcctgaaa
     6121 cagaagaata tggtatctca agttttatct atcgaaatcg gttactcctt gacgcacaaa
     6181 aatttgccca tcttacggat aaaaattttc ctggcaatat tacaagagca aaagggtttc
     6241 tttggtttgc ccaacatagt gatttttcga cgatctttga aacatcgggt agctataaaa
     6301 gtattgaatc attaaattat tgggtagcta gtttacctaa aattgagtgt caaatgatta
     6361 tacaaaaaaa tcccgaattg aaggaagcct ggcatccgtt gtttggtgaa cgcattaata
     6421 aactagtttt tattggtatg aagatggatc aacaatgtat taaggaacaa cttgagggtt
     6481 gtttaactga ttctcttaat gcagataatt tccgaaaaat ttcaccattt ccatggaaaa
     6541 agtctaatat gtttagccag atatcgttaa tgaaattttt aagaaaagct ggtgtcaaga
     6601 taggcgatgt cataattatt ggcattttga ttattacctc ttttattccc ttatttgtat
     6661 tctctataga aaaggagaat gaaacaactg aagatgctat caaatatgcc atcgttcgaa
     6721 ttgatggcaa ggaaatggat cgcttcgatt tggataagat caatcataaa ctcgtgaatt
     6781 atcatcctgc gaagggacaa tataatattg ttgagattaa agatggaaga attcgagtta
     6841 aagaagataa tagtcctgat caaatcgcgg ttagaactgg atggatctca aaacccggtc
     6901 aaacgagtat ttgtttaccc cataagttag ttattagtat tgagaaaaaa gaaagcacag
     6961 attactacat ttactaatct ggaaatagat gaggaggttt ttaataagaa aaagatatta
     7021 ttattaataa tagcaggggc tttattaagt gtgttaagcg cttgtggaca agaaaaagaa
     7081 gcggtgacaa caggcacaag tatctttaac gggacctgca atggtttagt taacagtcgg
     7141 ttagattact tattttggct cttcgtcaat gagtgttggt gaagaaattc aatagaataa
     7201 tcgagatact cacacggcta gacagctatc ttgtctagcc gtgttgttgt tctggcttta
     7261 gtcagttggg tagtattctt agttttctta aaatattttc ctcgatataa aaagattcgt
     7321 tctcggtagt tttgaaagtt tctatagcca taggcaattc gttttagtac tttgatatgg
     7381 ttattcaaac attctatagg gccgttggaa tagggaacgc gcaacgctcg tttgatttct
     7441 gtttggtatt tcttgaaggt cgtgaacact ggttggtagt gctctggcaa ctccttgtag
     7501 tcttctttta ggagcgcgac gaattcagga acatcttgtc ttctgataac ggaaagaaaa
     7561 tcctggtaga cttcgtagcc tcgctttaag gaatcatcat aagataatag ccgatcgact
     7621 atctctgctt ctgtcagatg atctcgaaat gacggacgcc agtagcgatg ctgacctttc
     7681 aattcccacg cattcttttg aagtagtttc cagtatttct ttaagtgctt cgaccgacga
     7741 tccccttttt taaaggtctt cattacttga attcggtggt tggtaaaagc ccgaccaatg
     7801 tgttggctaa tatgaaagcg atcggcgacg attcgggcat tcggaaaaca ctctttcacc
     7861 aatgtgcgat agggtgtata gaaatctgaa accactagac gcacttgttc acggactttt
     7921 atagggtagc gcaagaaata gtcgcgtaaa ggattgagtt gccgattttc cacaatatca
     7981 atcagttgat gggtcttgcc gtccatcatg acaaagctca tggctcccga gacatttttc
     8041 actgatttga attcatcgaa acacaccact tctggtaaca gctggctcaa agaaatggtt
     8101 ttaggccgaa ggctgcgaag tattcgaatg acagtggtcg gagaaatgtg cttcatacgt
     8161 gcgatcaaag acatagaaag aggttcgctt aatagctcaa gaatggcttg tttgactctg
     8221 cgagcaatgg agcagcgacg atcactaact gaatcttctg ctaaaaaagt ctttccacag
     8281 tgtttacatt tgaagcgctg tttgttaatc cgcagaatgg tcttgtagtt actgacatca
     8341 ttcaataaga tggatacttt tttccagccc tatttaatga tcgtttgttc attttgatag
     8401 tggcgatgaa agcattcctc cggttgatag gtcaatgtgc catcaaaaac aaaacatgtt
     8461 tcgtcattga ttcgctcttt gcgaaaggca tcttcatgaa agataagatt taggtctaga
     8521 atatctagta actctttggt aaactctgaa taggacatct tgaaacactc ctttgaatgg
     8581 ttttcgtcga ctttattcta aaggatttta aggtgtctgt ttgcgtttat tagaaaaaag
     8641 aaaaagtatt ggcaacagaa ggcgtcacca acactagata ttatagatcc cttattttct
     8701 tcgcttcaaa ttgtcaagga gtcatcataa attttgtatg gattcttttg tttgggtagt
     8761 agtcttctaa ataatcttgg attaacacct tcatctctat tttactttta tatttgttgg
     8821 agtcgattaa ttctcatttg attgatcgat gaaacgcttc aattactgca ttgtcaacag
     8881 gagtcccagt ttggctcact gaatgtgtga tgtggtggtc gttcaacagc tcttgataaa
     8941 taaaagaccg atgctggcta cctctatcgg aatgaattac cgtaggcaat tctccttaat
     9001 aaatttgtaa tggacggacg acaatctctt ttttcatgtg tgaatctacc atatagtaac
     9061 gtagaatacg agttgctaag tcaatatagg tggacaaata gaggcgaccg tcattacatg
     9121 gaatgtaagt aatatccatc acccaaacag catcaatttg gttctgttca aattatcgat
     9181 tcaacatttt tttaccattt ctttgattgt tttttctcta ccatgagaag atttgcgtcg
     9241 atgaaatcct cttgtgcata aattcatttc acgcataaga acagctacaa cacgtttatt
     9301 gatttcgatt ccttcatcgt aaagtttttg agtgatccct ggagatccgt atcgatcttt
     9361 gtgttcgtag aacactcgtc ttcctctttt agtcgtttat tttcctgttc aagaatgtag
     9421 aaatctgctt tatcaggtaa tctttttcct tgtccagcaa aagcattttt cccgtatcga
     9481 cgatattctg aaacccatcg ggaaattgtt ggacgagaga tattgtattc ttcagcaatt
     9541 ttcttggtag ttactttttt ctcaaggata tcctgtgaaa tctttagttt aaatttacga
     9601 tcatatgttt ttctcaatag agttcattcc ttccggtaac gtaactgata gtcaagaaaa
     9661 gtgtatcata gcccaactta gtatcagggt ttgtcaacta aactgtggaa gttgatagat
     9721 aagagttttt aacaaccaaa attctcttga ctatttttga aatcagacaa attttgattg
     9781 gacacatagt tgaataaatc taccgtcgtt acggaaggta aatcgcatgc ttttcaattc
     9841 tagaggagaa gtcctcgttg aagtaccata gcccagttct ggattcgacc tcctgcccac
     9901 ttcatacgta aataaagtaa ttttaggagt gagactgtga caaaagttat ttttaagcta
     9961 aaaacgaatt aaaaaacgtt gataattaaa atcattcgtt ttagattcta tgaatcatta
    10021 tagaa
//
LOCUS       DAZKLD010000108         9894 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.108, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000108 DAZKLD010000000
VERSION     DAZKLD010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9894)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9894)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9894
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.108"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1188
                     /locus_tag="ACG8KR_002209"
     CDS             <1..1188
                     /locus_tag="ACG8KR_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFQ0201538.1"
                     /translation="IYKGTLLYKPEECSHCLCVVPSRIIRWGTTTVRLLLNDASEYRT
                     YLELKKQRFKCKSCQRTFVADTSVAEKHCFISQKVRWSVIARLKENTSMTEIARQKNI
                     STSSVYRVMKRFYRPLNPFKQALPKVLCFDEFKSVRSVASAMSFIMMDGQSHALLDIV
                     ENRRLPYLECYFSRFSLATREAVQWIVIDMYAPYVSLVKKLFPNAQLIIDRFHIVQHI
                     GRTFLNHRVKETTARLKGTSHSQRGLGKKLKRYWKLLQKEEAKLNYEKRVWRASFKDY
                     LTESEIVDRLLSACPNLRQGYQLYQDILYAVKKRDQSLFEDCLNREVSGLPETYTTTL
                     RTFKKFLPQIQNALHYSYSNGPLECLNNHIKVLKRNAYGFRSFYNFKLRIMIRHGKTF
                     LTK"
     gene            1308..1508
                     /locus_tag="ACG8KR_002210"
     CDS             1308..1508
                     /locus_tag="ACG8KR_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HFQ0201539.1"
                     /translation="MIEEEQKNIWDRYYKADPSSKNTKIGESGLGLSIVKELVRLHHG
                     SIEVTSRKNEGTTFLIQFPKEK"
     gene            1584..1781
                     /locus_tag="ACG8KR_002211"
     CDS             1584..1781
                     /locus_tag="ACG8KR_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0201540.1"
                     /translation="MVECLKNKKMEANFVKAVISGSIVFQEEYRDMEKDFSKKGIELI
                     DYPRSCPILDKEYPEILTTFF"
     gene            complement(2078..3625)
                     /locus_tag="ACG8KR_002212"
     CDS             complement(2078..3625)
                     /locus_tag="ACG8KR_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HFQ0201541.1"
                     /translation="MNITFLETSDMHGYVSPTDYSGRTDLALGTAKVSSKLKELRAKA
                     DGPVITIENGDFIQGSPLSYYLAKEYGSAKELTDLINQMSYDVQVLGNHEFNYGVDYL
                     EEAIKNYQAPILGANMVNKNDQPYFGNAYKIIEKVGVKIAILGLTTQYIPHWEKPETL
                     AGITFKSIVETAKEYVPLLRKQADLVIVSYHGGFEKDLLTGEDTEALTGENEGYELLQ
                     QVEGIDALFTGHQHRKIAETVHGVPVVQPGYRGEFIGEIRLTVEKKEGQVVVTDHQVA
                     LHSVEKSEPDPEILAAIVPVEEKLEKWLDQPLGKVNGNMRINDPMQARLEEHPYVEFI
                     NRVQLFASGSEISGTALFNNEGKGFGETITMRDVITNYIYPNTLAVIQINGADLRAAL
                     EQTANYLAVENNQIVFNPAFIQPKPQYYNYDMYEGIQYTIDMKQPVGQRITELRFKGH
                     PVLPQQMLEVVTNQYRAIGGGNYAMFGAEKIVREIQIDMTELIADYLNIHPRIEAKVN
                     HNFQVIL"
     gene            complement(3709..4512)
                     /locus_tag="ACG8KR_002213"
     CDS             complement(3709..4512)
                     /locus_tag="ACG8KR_002213"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFQ0201542.1"
                     /translation="MNILHISDIHFRREYEACEEGYKGMLATMQNPLIPLEQCIHHLL
                     QQTPLDLVIISGDLTEDGEIDDYRYLKTWLENALGETTILVTLGNHDIKEHFWVGWCD
                     EAASSAPYNQIVKYPEFTVISFDNSSYGYADGIVDDQQFQWLKKALEQEKDQPIFLVT
                     HHHLLSHQSSIPKWPGTERFLKLIAPYDIRCILNGHTHHTFTDEINGIPYFTVSSMSF
                     VGEDEGDGFVRFEERHGYNLYHLDQGRIVRQTTENFIPGNILKTLQMKD"
     gene            complement(4515..5297)
                     /gene="phnE"
                     /locus_tag="ACG8KR_002214"
     CDS             complement(4515..5297)
                     /gene="phnE"
                     /locus_tag="ACG8KR_002214"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005233297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HFQ0201543.1"
                     /translation="MMLREMNRRTLLSLVALVILVILSAATLDYSGLSTFSPSMGMDV
                     LRGLGQPDWQFLYDGSGEDLISLLLLTIGIACLGTMIATVLAIPITLISAVNLWQAHP
                     WVTKIGKLICNVLRAFPELVFAIIFVKVVGPGPFAGVMAIGVHQIGMLGKLFTEEMEA
                     MDERLVEEMHAVGANFWQTIFFVRIPYLMPIYCSLALNHFEIAVRSAATLGLVGAGGI
                     GAPLIFAIQTRSWSKVSIILIGVVVTVFILDQITGIIRKRLR"
     gene            complement(5294..6073)
                     /gene="phnE"
                     /locus_tag="ACG8KR_002215"
     CDS             complement(5294..6073)
                     /gene="phnE"
                     /locus_tag="ACG8KR_002215"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010743453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HFQ0201544.1"
                     /translation="MSLKETVHFKWYKHLLILLFLIVCFQGSAWITSADFSQVFQNSN
                     QMTVFLSRFIHPDFSYLPKLVEPMVKTLQMSLLGTVIGLILAIPVSFLATTVVTENRL
                     LSTIFRFLLGLVRTIPTLLLAALFVAVFGIGEATGVLTIAVFTFGMVSQLMFQAIETI
                     DFGPIEAAAAVGANKMQIAIWSIAPQVLSQFASYAFYAFEVNVRASTVLGYVGAGGIG
                     VILNSSLALLNYERVSIIILTILVTVAIVDKLSERVRRSLL"
     gene            complement(6045..6836)
                     /gene="phnC"
                     /locus_tag="ACG8KR_002216"
     CDS             complement(6045..6836)
                     /gene="phnC"
                     /locus_tag="ACG8KR_002216"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HFQ0201545.1"
                     /translation="MIIFDNVSKVYPNGVVGLKDIDLTIEDGEFVSIIGLSGAGKSTL
                     LRSINRLAETTKGDIRINGTSITKANKKELRQIRRQIGLISQSFNLVKRSTVQKNVLS
                     GRLGYYSTWKSILGLFTKEDYQQTKEALEKVGLSDKLQTRSDELSGGQQQRVSIARAL
                     VQQAPIILADEPVASLDPITTQKVMMDLKNINQTMDKTVIVNLHSVPLAREFSTRVLA
                     LKAAQVVFDGRPDDLTDERLEQIYGNAIFEEKAGEIDELERNRSL"
     gene            complement(6897..7826)
                     /locus_tag="ACG8KR_002217"
     CDS             complement(6897..7826)
                     /locus_tag="ACG8KR_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010743455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate/phosphite/phosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HFQ0201546.1"
                     /translation="MQFKQLAKGFIGVMLLTTLAACGSGNTSSSSEKESKEKLVVQFV
                     PTNNDGSMEAKAKPFAKYLSKKLDREVEVTLATDYSTIVEAMSSGQVDVGIMPPAAYV
                     QAKDAGAADAILTSQLGDYDQETGLPLKDKLTNTFKGEVLVRADSGLNKLTDLKGKNI
                     ATLSPNSASGYIYPVAELKDAGIDPTADTKMTTVNDIPSEITAVLNKQMDAAFVFEGA
                     RNVFASNFPDNDLFKDLKVLYLTKGDIPNDAIAVQPDMSKDLKAKIKETFLNMKDDKD
                     GKEAMALWGHQGYEEAADSAYDTIRDYTKKAAE"
     gene            8021..8704
                     /locus_tag="ACG8KR_002218"
     CDS             8021..8704
                     /locus_tag="ACG8KR_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HFQ0201547.1"
                     /translation="MKKIHDTKQLTAYVEKHNLQDYMDSDILAMASLLFFEKEEHLIQ
                     TGKTSDYLYFLVDGTVMVYSYSSDTQNICIDYAKPATPLGEASSLWELSPKSSVKAVT
                     KCICISIPLNQYRTQLQKDVRFLQNICQLLSYRLNSGINLANSLTEPVETRLAKFILT
                     HHENERFSFRLTTCASILNVSYRHLLRTITNFREENIIEKKKNYYLIHDIEALEDLAE
                     NISSPVKAE"
     gene            8865..>9894
                     /locus_tag="ACG8KR_002219"
     CDS             8865..>9894
                     /locus_tag="ACG8KR_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family protein"
                     /protein_id="HFQ0201548.1"
                     /translation="MKKILVSLLFLVSLVTLVGCSQSQGEKQSEKEKSKIALVLGFGG
                     VDDRSFNQAGWEGLEAWGKEKKLTAKKDFTYIETNNPEDNSTNIDQAIRNGFTTIISL
                     SYLQAEELENAAKDNPKLKFALVDGEITGKKNISSATFKDQESAYLAGLAAAYTTKSD
                     QVGFIGGMEGPVIQRFQSGFEAGVKAGATALNKEITVNVQYAASYDSLDKGKAIAASM
                     YKEGVDILYQAAGSTGAGVFQEAKARNENDYSRKVWVIGVDRDQADEGDYKMENGETG
                     NFTLTSTKKNVGAEVKEFAETSHNDKFPGGKHLTFGLKEEGVDLTKGQLSEEAYQAVS
                     DAKEKIISG"
BASE COUNT         3018 a         2029 c         1968 g         2879 t
ORIGIN      
        1 atttacaaag gaactcttct gtacaagcca gaagagtgtt cccattgtct ttgtgtcgtc
       61 ccctctagga tcattcgttg ggggacgaca accgttcggt tgttactgaa tgatgcgtct
      121 gagtatcgca cttatctgga gttgaagaaa caacgattta aatgtaagtc ttgccaaaga
      181 acattcgtag cggatacctc tgttgctgaa aaacactgtt tcattagtca aaaagttcgt
      241 tggtctgtca ttgctcgctt aaaagagaat acttccatga cggaaatcgc tcgtcagaaa
      301 aatatttcca cttcttcggt ctatcgtgtc atgaagcgat tctaccgccc attgaatcct
      361 ttcaaacagg ctttgccaaa ggtcttatgc tttgatgaat tcaaatcggt tcgaagtgtt
      421 gccagtgcaa tgagttttat catgatggac ggacaaagcc atgcattatt ggatattgtc
      481 gaaaatcgac gcttgcctta tttagaatgc tatttttctc ggttttcatt agcgactcgt
      541 gaagccgttc aatggattgt tattgatatg tatgcgcctt atgtttcatt ggtgaaaaag
      601 cttttcccga atgctcagtt gatcatcgac cgtttccaca tcgtccaaca tattggcaga
      661 accttcttga atcatcgagt gaaagaaacc acggcacggc ttaaaggaac ttcccactct
      721 caacgagggt tagggaaaaa attgaaacgc tattggaaac tcctacaaaa agaagaagca
      781 aagttaaact acgaaaaaag ggtttggcgt gccagtttta aagattattt gactgaaagt
      841 gagattgtcg atcgactgtt atctgcttgt cccaacctgc gccaaggcta tcagttatac
      901 caagacattc tttatgcggt aaagaaaaga gatcagtcat tatttgagga ctgtttaaac
      961 agagaagtca gcggtcttcc tgaaacctat acaaccactt tacgaacgtt taaaaagttt
     1021 ctgccacaga tccaaaatgc gcttcactac agctattcaa atggaccatt ggaatgttta
     1081 aacaaccaca tcaaagtatt aaaacgcaat gcgtacggct ttcgcagctt ttacaatttc
     1141 aaactacgga tcatgattcg tcatggcaaa acatttttaa ccaaataaac agaacagaaa
     1201 gagagtttct tctttctgtt ctgtccaaat tattctaaga tttctcttta ccaacatcat
     1261 ttgacaaaga gcctgtattt attactgtcg aggacaatgg catcggcatg attgaagagg
     1321 aacaaaaaaa catttgggat cgctattata aagccgatcc gtcaagtaaa aatacaaaga
     1381 taggcgagtc aggtcttgga ttatcaatcg tgaaggaact agtgcgcctg caccacggtt
     1441 caattgaagt gaccagccga aaaaatgaag gaacgacctt cctgatacaa tttccaaaag
     1501 aaaaataaaa ttaccagtaa aaaaccgtgg agagatagct gctattgatt tttgctatgt
     1561 cttttctttg agaggtatcc ggtatggtgg agtgtctgaa aaataaaaaa atggaggcca
     1621 attttgtgaa agctgttatt tctggaagta ttgttttcca agaagaatat cgagatatgg
     1681 aaaaagattt cagtaaaaaa ggcattgaat taattgatta tcctcgctca tgtccgatcc
     1741 tagacaaaga gtatccagaa atcttgacga ctttttttta aatatcgcag cagcggattt
     1801 attttttgta tttaatcaag atcggaaagg ggtattaggc tatattggtg ccgcaacttt
     1861 ttcagaaatg aagtattgtc tgatgcaaaa tcttattcat ggaaaaagga tcaaaatagt
     1921 cttatttaag gaaccatcag aggaaaattt tttgttttga tgaggtagat cgctggctaa
     1981 agaatggctg gatggaaatc agacagccta acaatttggc ataacaaagg tgtgggcaaa
     2041 aaactgctca cacctttttg aattttttat ttatatacta caaaatcact tgaaaattat
     2101 gattcacttt tgcttcgatg cgtgggtgaa tattcaaata gtcggcaatt aattcggtca
     2161 tgtcgatttg gatttctcgg acaattttct ccgcaccaaa catcgcataa tttcctccgc
     2221 cgattgcccg atattgattt gtcaccactt ccagcatttg ttgcggaaga acggggtgac
     2281 ctttaaaacg aagttcggta atccgttgtc caacaggctg tttcatatca atagtgtact
     2341 gaatcccttc atacatgtca taattgtagt actggggctt aggctgaata aaggctggat
     2401 tgaatacgat ttggttattt tctacagcta aataatttgc ggtctgctcc aacgccgctc
     2461 tcaaatctgc accatttatt tgaatcactg ccagagtatt cggataaata tagttggtga
     2521 tgacgtcccg catcgtgatc gtctcaccaa agccttttcc ttcattattg aataacgcag
     2581 ttccagaaat ctctgagccg ctggcaaata attgtacacg attaataaac tctacatagg
     2641 gatgttcctc aagacgagcc tgcatcggat cattaatacg catatttccg ttcactttac
     2701 ctaagggttg atccaaccat ttctctagtt tttcttctac cggtacgatc gctgcaagaa
     2761 tttctggatc aggctctgat ttttcaactg agtgtaaagc aacctggtga tctgtaacaa
     2821 ccacttgccc ctcttttttc tcgacggtaa gacgaatttc accgatgaac tcgccacgat
     2881 agcctggttg aacaacggga actccatgaa ccgtttcagc aattttccgg tgctgatgac
     2941 cggtgaaaag ggcgtcaatg ccttcaacct gctgcaatag ctcgtagcct tcattctctc
     3001 cagttagcgc ttctgtgtcc tcaccggtta acaagtcttt ttcaaatccg ccgtggtaac
     3061 taacgattac caagtctgct tgtttcctta atagaggaac atattctttt gctgtttcga
     3121 cgatactctt aaacgtaatt cctgctaagg tttctggctt ttcccagtga ggaatgtatt
     3181 gtgtcgttaa tcccaagata gcaattttta cacccacctt ttcgataatc ttataggcgt
     3241 ttccaaagta tggttggtca tttttgttca ccatattggc tcctaatata ggcgcttgat
     3301 aattcttaat cgcttcctcg agataatcca caccatagtt aaactcatga tttcccaaca
     3361 cttgaacatc atagctcatt tggttaatca agtctgtaag ctctttggct gaaccatatt
     3421 ctttagcaag ataataactc aaaggagacc cttggatgaa atcaccattt tcgatcgtaa
     3481 ttaccggtcc atctgctttt gcccgtaatt ccttcagttt cgacgatact ttagccgttc
     3541 ctaaggccag atcggttctg ccggaatagt ctgttggtga aacatagcca tgcatatcac
     3601 ttgtctctaa aaacgtaatg ttcattctgc gtgctccttt agctgactca aaatagaaaa
     3661 accgaagcgt attcagctgt tcggtctctt tttttgattt ataatgcctt aatccttcat
     3721 ttgaagtgtt tttaaaatgt tgcctggaat aaagttttct gttgtctggc gaacaatcct
     3781 tccttgatcg agatgataaa ggttataccc atgacgttct tcaaagcgta cgaagccatc
     3841 gccttcatct tcaccaacaa aggacatact cgatacagta aaatagggga tcccattaat
     3901 ttcatcagta aatgtgtgat gggtatgacc gtttaggata cagcgaatat cgtaaggtgc
     3961 gattagcttc aagaaacgtt cggtaccagg ccattttggt atactgcttt ggtgagacag
     4021 tagatgatga tgggtgacca agaaaatcgg ttggtctttt tcttgctcta aggctttttt
     4081 cagccattga aattgctgat cgtccactat accatctgcg tatccatagc ttgaattatc
     4141 aaaggaaatc actgtaaact caggatattt gactatctgg ttgtacgggg cagaagaggc
     4201 ggcttcatcg caccagccta cccaaaaatg ttctttgata tcgtgattgc ctagagtaac
     4261 aagaatcgtc gtttctccta gcgcattttc cagccaagtc ttcaagtatc gataatcgtc
     4321 tatttcgcca tcttcggtta aatctccgct gataatcaca aggtccaagg gtgtttgctg
     4381 caggagatga tgaatgcatt gctccagagg gataagggga ttctgcatcg tagccagcat
     4441 tcccttatag ccttcttcac aagcctcata ctctctgcga aagtgtatat ctgatatatg
     4501 caatatattc attcttatct caatctcttt cttataattc cggttatttg atctaaaata
     4561 aacacggtta cgactacgcc aatcaaaata atactcactt tgctccaaga ccgagtttga
     4621 atcgcgaata tcaatggagc accgattccg ccagcgccaa ctaagcctaa ggtagccgca
     4681 ctacgaaccg ctatttcaaa atgatttaat gctaaagaac aataaatcgg catcaaatag
     4741 gggatgcgaa caaagaaaat cgtttgccaa aaatttgctc ctaccgcatg catttcttca
     4801 acaagtcgct catccatcgc ttccatttct tctgtaaaaa gtttgccaag cattccgatc
     4861 tgatggacgc caatcgccat aacaccagca aaggggccag gtccgacgac tttcacaaaa
     4921 ataattgcaa aaactagttc aggaaaagct cgtaaaacat tacagataag cttgccaatt
     4981 tttgttaccc atggatgtgc ttgccagagg ttaaccgcac tgatcaaggt aatgggaatc
     5041 gctaataccg tagcgatcat tgtccctaaa caggcgattc caatggttaa taatagcaaa
     5101 ctaatcaaat cctcgccgct gccatcatat agaaactgcc aatcaggctg acctaaccct
     5161 ctcaaaacgt ccattcccat ggaaggagag aaggtgctta atccagagta atccagagta
     5221 gcggccgaca aaataacgag aatcactaaa gcaactaatg ataaaagggt tcttctgttc
     5281 atttcacgaa gcatcataac agggacctcc tgacacgttc acttaattta tcaacgatcg
     5341 cgacagttac taggatcgtt agaataatga ttgaaactcg ttcataattc aaaagcgcaa
     5401 gtgaggaatt tagaataacg ccaataccac cagctccgac atatccaagg acagtagaag
     5461 cacgaacatt cacctcaaac gcgtaaaaag catagctcgc aaactgactc aaaacttgtg
     5521 gagcgatcga ccaaatagcg atttgcattt tatttgctcc aacagccgca gcggcttcaa
     5581 tcgggccaaa gtcaatggtt tcaattgctt gaaacattaa ttgtgacacc ataccaaaag
     5641 taaagacagc gatagtcaac acgccagtcg cttcaccaat tccaaacaca gcaacaaaaa
     5701 gcgcggctaa aagcagggta gggatggtcc gtactaaacc taaaagaaaa cgaaagatgg
     5761 tagaaagaag acggttttct gtcactacag tggtcgctaa aaaactaaca gggatcgcta
     5821 aaattaagcc aatgaccgtt ccaagcaaag acatttgaag tgttttcacc ataggctcga
     5881 ctaatttagg taaatagcta aagtctggat gaatgaagcg tgataaaaag acagtcattt
     5941 gattggagtt ttgaaaaacc tgagaaaaat cagctgacgt gatccaggca cttccttgaa
     6001 aacaaacaat taagaacaga agaataagca ggtgtttata ccatttaaag tgaacggttt
     6061 ctttcaagct catcgatttc gcctgccttt tcttcaaaga ttgcattgcc ataaatttgc
     6121 tctaagcgtt catcggttaa gtcgtctgga cgaccatcaa agacaacctg agcagctttt
     6181 aacgcgagca cacgcgtaga aaactcacga gccagaggga ctgaatgcag attgacaatg
     6241 actgttttat ccatcgtttg attgatattt tttaggtcca tcataacctt ttgagtcgtg
     6301 attggatcta atgatgcaac gggttcatcc gctaaaataa tgggggcttg ctgcacgagt
     6361 gctcgcgcaa tggatactcg ctgttgttgc ccaccactga gctcatcact gcgtgtctgc
     6421 aatttatcag acaaaccaac tttttccagt gcttcctttg tctgctggta gtcttccttc
     6481 gtaaaaagac ctaaaatact tttccaagtg gagtaatagc ccaaacgtcc agatagcaca
     6541 tttttctgaa ccgtactacg ctttaccaga ttgaagcttt gcgagattag accaatctgg
     6601 cggcgaatct gtcgcagctc ttttttattc gcttttgtaa tagaggttcc attaatgcga
     6661 atatctcctt tggtggtttc tgccaagcga ttgatactgc gcagcagagt gctctttcca
     6721 gcaccactca accctatgat ggaaacaaat tctccatctt caattgttaa gtcaatatcc
     6781 ttcaagccaa caacgccatt gggatagacc ttcgaaacat tgtcaaaaat aatcattatc
     6841 cttctccatt tttttaagtg taagcaaaaa aaagcttgct gacacttact gatgttttat
     6901 tctgctgctt ttttcgtgta gtctctgatc gtgtcatagg cagaatcagc agcttcttca
     6961 tagccctgat gcccccacaa agccattgct tctttgccat ctttatcatc cttcatattt
     7021 aagaaggtct ctttaatttt cgcttttaaa tcttttgaca tgtcaggctg tacagcgatt
     7081 gcgtcattcg gaatatcgcc ttttgtcagg tacaggactt ttaaatcctt aaataaatca
     7141 ttatctggga aattcgaagc aaaaacattt cgcgcgcctt caaaaacaaa cgccgcatcc
     7201 atttgcttgt tcaacactgc ggtaatctca ctcggaatat cattcacagt ggtcatcttt
     7261 gtatctgcag taggatcaat cccagcatct ttcaattcag cgacaggata gatgtagccg
     7321 ctagcagagt taggacttaa ggttgcgata ttttttcctt ttaaatcagt taacttattc
     7381 aatccgctat cggcacgaac caatacttct cctttaaagg tgttcgtaag tttgtccttt
     7441 aatggcaagc cagtttcttg atcataatcg ccgagctgag aagtcaggat tgcatctgca
     7501 gctccagcat cctttgcttg aacataggct gcaggcggca taattccgac atcgacttga
     7561 ccagaagaca tcgcttcgac gatcgttgaa taatcagtag ctaacgtcac ttctacctca
     7621 cgatctaact ttttcgataa atacttagca aatggttttg cttttgcctc catactacca
     7681 tcattgtttg ttggaacgaa ttgaacaact aatttttctt tgctctcttt ttctgagctg
     7741 ctacttgtat tcccagaacc gcaggccgcc aaagtggtca ataacatgac tccgatgaac
     7801 ccttttgcta actgtttgaa ctgcattttt ctcctcctgt tggatataat ttctaatacg
     7861 tctaaatcat agcagggaat caaaatcaat gaagtgacgc acgtcctatt ttacacaact
     7921 tttacataca atttacaaac aattataaat aattattcgt attgagccga aaaacaatct
     7981 aagctataat aaagaaaaac ttcttagagg tgacgatttc atgaaaaaaa tccatgatac
     8041 gaagcaatta actgcttacg ttgagaagca caacctgcag gattatatgg attcagatat
     8101 attggcaatg gcttctttac ttttttttga aaaggaagag cacctcattc aaacgggaaa
     8161 aacctcagat tatttgtatt ttttagtgga tgggacggta atggtgtact cgtattcttc
     8221 tgatacacaa aatatttgca tcgattacgc aaagcccgct acaccactcg gcgaagcctc
     8281 ttcattgtgg gaattatcgc cgaaaagcag cgttaaagca gtgactaagt gcatctgtat
     8341 cagcattccg ctaaatcaat atcgaacaca gcttcaaaaa gatgttcgct ttttgcagaa
     8401 tatttgccag ttactaagtt atcgtttaaa ttccgggata aatttggcga actcactgac
     8461 agaacctgtt gaaactcgtt tagcaaagtt tattttgacc catcacgaaa acgaacgttt
     8521 ctcatttcgg ttaaccacct gcgcctcaat cttaaatgtc agttatcgac atcttttacg
     8581 aacaatcacg aattttcgtg aggagaatat tatcgaaaag aaaaagaatt attacttgat
     8641 tcatgatatc gaagctttag aggacttagc tgagaatata agtagtccag taaaagcaga
     8701 ataacagcta taaaaaaaca gcagtcagaa gtgattgctg gttttttgat agtggagaaa
     8761 ttgggaaacg gaaaatcttc gttgaagagt agtgttctat atattggtat gctatacttt
     8821 taaaagttca atgtgtcaag acacataaaa aggggagaaa aattttgaag aaaatacttg
     8881 tttcattgtt gtttttggtg tcattagtaa cgttagtcgg ctgtagtcag tctcaggggg
     8941 agaagcaaag tgaaaaagag aaaagtaaaa tcgctttggt tcttggattt ggcggggttg
     9001 atgaccgctc cttcaatcaa gctggctggg aaggattaga ggcttgggga aaagagaaaa
     9061 agctgacagc aaaaaaagat tttacgtata tagaaacgaa caatccagag gataacagta
     9121 ccaatatcga tcaggcaatc agaaacggct ttaccaccat tattagcttg agctacttgc
     9181 aagcagagga actggaaaat gctgctaaag ataatccaaa gttgaaattt gccttggtag
     9241 atggtgagat tactgggaaa aagaatatct catcagcaac ctttaaagat caagaaagtg
     9301 cgtatttagc aggactagct gccgcatata cgacaaaaag tgatcaagta ggctttattg
     9361 gtggaatgga agggcccgtg atccaacgtt tccaaagcgg atttgaagct ggcgttaagg
     9421 caggggccac agcactgaac aaggaaatta ctgttaacgt acaatatgct gcatcctatg
     9481 attcgctaga taaaggaaaa gctattgctg cttctatgta caaagaggga gtcgatattc
     9541 tttatcaggc ggcaggaagt actggagctg gggtgttcca agaagcaaaa gcccgcaatg
     9601 aaaacgacta tagcagaaaa gtatgggtga ttggtgtaga tcgcgatcaa gcggatgaag
     9661 gggattataa aatggagaac ggcgagacag ggaatttcac gttgacttct acaaagaaaa
     9721 atgtcggtgc agaagtcaaa gaatttgcag aaacttctca taacgataaa tttcctggcg
     9781 gcaagcattt gacctttggt ttgaaagaag aaggggtaga tctgacaaaa ggacaattat
     9841 cagaggaagc ctatcaggca gtttcagacg caaaagagaa aatcatcagt ggtg
//
LOCUS       DAZKLD010000109         9806 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.109, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000109 DAZKLD010000000
VERSION     DAZKLD010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9806)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9806)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9806
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.109"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(80..2200)
                     /locus_tag="ACG8KR_002220"
     CDS             complement(80..2200)
                     /locus_tag="ACG8KR_002220"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFQ0201549.1"
                     /translation="MKCIKTPAFLNKRLGFFSLLVVLLWLKTIFAYLVDFHLGIESGI
                     QYFILFINPIATTLLLLGIALYIRRPRVAYITMMVIYFLLTLLVFSNVTYYREFTDFI
                     TINTILGAGKVASGLGESAIRLFRPYDVIYWIDFIILTVLLIAKKIKLDPRPIRARMA
                     FAISTLAVMIFSGNLFMAEADRPELLTRTFSRDYLVKYLGLNAFMVYDGVQTYQTNQV
                     RAEASPNDMKEVENYVKEHYAAPNDDLFGLAKGKNVIYIHLESFQQFLIDYKLTDENG
                     LQLEVTPFINSLYHSNSTFSFDNFFHQVKAGKTSDAETLMENSLFGLNQGSLFTQLGG
                     KNTFQAAPDILSQTQGYTSAVFHGNAGTFWNRNETYKNLGYDYFFDSSYYDVNDDNSF
                     QYGLHDKPFFQQSVQYLERLQQPFYSKFIAVSNHYPYSKFTNDEAGFPLANTNDETIN
                     GYFATANYLDTAVQEFFNYLKESGLYDNSVIVLYGDHYGVSNSRNKNLAELVGKTSST
                     WTNYDNAQMQRVPYMIHIPGQTQGGINHTYGGQVDALPTLLHLLGVDTKNYIQLGQDL
                     FSKDHEQLVSFRDGDFVTPKYTYYGGTIYSNETGEAITEPTEEVQNEIDSLKEKVDKQ
                     LAVSDQINNGDLLRFYTNSGLDPIDTSDYDYKNGLEKLKEAEKSLGDKSTSVFSEHKG
                     QSTVDLYKTQTYQELHGTTTSSSE"
     gene            2430..3608
                     /locus_tag="ACG8KR_002221"
     CDS             2430..3608
                     /locus_tag="ACG8KR_002221"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HFQ0201550.1"
                     /translation="MKVKVKKRASNRLKKGYPLIQKEDLAHPQKETTQWVELVDSQNQ
                     FIAKGYLGKQNKGIGWVLSQQNIPFDQSFFEEFFVKSKEKRTFFFSDDSTTAFRIFNG
                     EGDGIGGLIVDYYAGYAVFSWYNETLYAHRELLIQAFREIYPEIVGGYEKIRFESVNL
                     PESQFIYGKEAPEPLIVKENGVKFAVYLNEGLMTGIFLDQKEVRGSLAEGFASGKTVL
                     NMFSYTGAFSVAAAMGGAIETTSVDLAKRSLKKTQEQFEVNGLDVDQQKIIVMDVFEY
                     FKYAKRKQFLYDMIVLDPPSFARNKKKVFRVAKDYGELVKDSLDILAEDGILIASTNA
                     ANVSIEQFQTMIEEEFHAKNVSFEQLALYRLPKDFQTTDTFLEGNYLKVFVYQIKNNH
                     "
     gene            3624..4382
                     /gene="aroD"
                     /locus_tag="ACG8KR_002222"
     CDS             3624..4382
                     /gene="aroD"
                     /locus_tag="ACG8KR_002222"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HFQ0201551.1"
                     /translation="MVWVKGLNIGSGRPKICAPLIGAVREEVLREAVLAREAGADLVE
                     WRVDHYREVFSFDEVAETLVLIHEMLDGLPLLFTFRTLAEGGEQDISIRQYRDLYEHV
                     IHTGLVDMVDIELFKVESLGKRLIEEIKSLNIPLIISSHDFKETPADPVLLYRLNVME
                     HFGADIGKLAVTPNNERDVLRLMELTRRANAFVSMPIITMSMGNLGKISRLAGGTTGS
                     VMTFGSLSSDSTSAPGQLPVKALKQIIQLLNEPL"
     gene            4405..4890
                     /gene="ybaK"
                     /locus_tag="ACG8KR_002223"
     CDS             4405..4890
                     /gene="ybaK"
                     /locus_tag="ACG8KR_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HFQ0201552.1"
                     /translation="MAKKKIVKTNAIRMVEQKKIPYTEHEYEWDESHLSASSVAEQLP
                     ESQSHIFKTLVAVGNVTGPLVAVIPGEAELNLKKLAKVSGNKKVEMLHLKDLEATTGY
                     IRGGCSPIGMKKLFPTYLDQIAESYEQIIVSAGRRGLQMELAPQDICALTSGQFADIK
                     Q"
     gene            complement(4961..6214)
                     /locus_tag="ACG8KR_002224"
     CDS             complement(4961..6214)
                     /locus_tag="ACG8KR_002224"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFQ0201553.1"
                     /translation="MEKLYDVIVVGAGTSGMMAAISAAEQGARVLLIEKNKKAGKKLL
                     MTGGGRCNVTNNRPVDDLIAHIPGNGKFLYSTFAQWNNFDIMNFFESQGVHLKEEDHG
                     RMFPVTNKSKTIIEALLNRLKELDVTLLFSTRVEKLIHKEHKIYGIRTEFEEFHAPAI
                     ILTTGGRTYPSTGSTGDGYKIVKRVGHTVTPLYATESPLISDEPYIQEKTLQGLSLQD
                     ITLRVLNKKGRVLTEHTMDLLFTHFGISGPAALRCSSFVNKELEKTGEPVTLSLDCFP
                     TQTKQELIHILTEKSKATKKNLVNAWHGILPERLLVFFLERLEMDHLTGQQASQKQIQ
                     DFVQLCKEFKLSINKTFPIEKSFVTGGGVSLKEIYPKTLESKIIDGLYFAGELLDVNG
                     YTGGFNITAAFATGHVAGMNAGQRA"
     gene            6330..7679
                     /gene="gdhA"
                     /locus_tag="ACG8KR_002225"
     CDS             6330..7679
                     /gene="gdhA"
                     /locus_tag="ACG8KR_002225"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HFQ0201554.1"
                     /translation="MTNATEYVQAIQEKLHQKDQGQEEFLQAIDEFMPTVMSFLDTHP
                     EYIEKNILELLTEPERVIQFRVPWQDDEGNWRVNRGYRIQYNSAIGPYKGGLRFHPSV
                     NLSILKFLAFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDNEIMRFCQSFMLELSKHIG
                     PSIDVPAGDIGVGAREIGYLFGEYKRLKKYDTGVLTGKPLDFWGSKIRTEATGYGLVY
                     YVKHLLNEEKDSFNQKTVFVSGSGNVAIYAIEKVQELGGKVVTCSDSSGYIYDPEGID
                     VKLLKEVKEINRQRLTEYANQRPSAIYHAGESVWTLKEKADIALPCATQNEIDESLAE
                     ILVENGIKIVAEGANMPCTVKAVSVLHEAGVWYCPGKAANAGGVAVSALEMSQNAQRL
                     TWEREQVDEQLDQIMETIYHTCRQTAAEYGDEKNLLLGANVAGFEKVAKAMAAEGLV"
     gene            7791..9137
                     /locus_tag="ACG8KR_002226"
     CDS             7791..9137
                     /locus_tag="ACG8KR_002226"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HFQ0201555.1"
                     /translation="MAHIHFDYSKVAPFVNEQELGYMQSQVTAAHNELREGTGAGSDF
                     RGWIDLPTNYDKEEFARIKEAAKKIQSDSEVLVVIGIGGSYLGARAAIDFLNHTFFNL
                     LDSNTRKAPQIFFAGNSISSTYIADLIEVIGDRDFSVNVISKSGTTTEPAIAFRVFKE
                     LLVKKYGQEEANKRIYATTDKAKGAVKVEADAEGWETFVIPDDIGGRFSVLTAVGLLP
                     IAVSGADIDALMQGAADASKAYSSDKLEENEAYQYAAMRNILYRKGKVTELLINYEPG
                     MQYFSEWWKQLYGESEGKDQKGIYPSSANFSTDLHSLGQFIQEGRRNIFETVVKVEKP
                     RKSITIPEQAEDLDGLGYLQGKEVDFVNTKAFEGTLLAHTDGDVPNLLVKIPEMDAYT
                     LGYTMYFFEIAVGISGYLNGINPFDQPGVEAYKKNMFALLGKPGFEDLAKELNERL"
     gene            complement(9445..9804)
                     /locus_tag="ACG8KR_002227"
     CDS             complement(9445..9804)
                     /locus_tag="ACG8KR_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFQ0201556.1"
                     /translation="MILIAFIGWKGWDYYQSTYVGKDYYAVVKAPMPAETDIKADNGE
                     VIGRGFKYNVDAYAENGEKRQLDFDVITSGDSANGSAYTEGTILQLKASEKRIIEKKV
                     ITSDKVPSSVKEKLGLS"
BASE COUNT         3130 a         1783 c         2009 g         2884 t
ORIGIN      
        1 attattctaa gatttctctt taccaacatc atttgacaaa gagcctaatt ttgtcgctgc
       61 tccttttaaa tttattttat cattcggaag aacttgtggt cgttccgtga agttcttggt
      121 acgtttgcgt tttataaaga tcaacagttg attgtccctt gtgttcacta aagacactag
      181 ttgacttgtc accaagagat ttttcagctt cttttaattt ctctaagcca ttcttgtagt
      241 cataatcact ggtatcaatt gggtctaatc cactattcgt atagaaccga agaagatcac
      301 cattattgat ttggtcagaa acagctaact gtttatcaac tttttctttc agtgaatcaa
      361 tttcattctg tacttcctca gtaggttcag taatagcttc accagtttcg ttactataga
      421 tcgtaccacc gtagtaagta tattttggtg taacaaaatc accatcacgg aaagaaacaa
      481 gctgttcatg atctttcgag aatagatctt gtcctaactg aatataattt tttgtatcta
      541 cacctagtaa gtgaagtaat gtaggcaagg catcaacttg tccgccgtaa gtgtgattga
      601 ttccgccctg tgtttgtcca gggatatgga tcatataagg gacacgctgc atttgtgcat
      661 tgtcgtagtt tgtccaagta gaacttgttt ttccaactaa ttcagctaga tttttatttc
      721 ttgaattcga gacaccgtaa tggtcaccgt aaaggacgat gactgaatta tcatacaagc
      781 cgctttcttt caaataattg aagaattctt gtacggcggt atctaaataa ttggctgtcg
      841 caaagtaacc gttgatcgtt tcatcatttg tattggctaa cgggaatcct gcttcgtcat
      901 tggtgaattt tgaataagga taatggttag atacagcaat gaattttgaa taaaatggtt
      961 gttgcagacg ttccaagtat tgtacggatt gttggaaaaa tggtttatca tgcaacccgt
     1021 attggaacga attgtcatca ttaacatcat aataagaaga atcaaagaaa taatcatatc
     1081 ctaaattctt gtatgtctcg tttcgattcc agaatgtacc agcgttccca tggaataccg
     1141 cactagtgta accttgtgtt tgacttaaaa tatcaggtgc agcttggaaa gtattttttc
     1201 cgcctaattg tgtgaacaag gagccttgat tcaagccaaa caatgaattt tccatcagcg
     1261 tttccgcatc gcttgttttt cctgctttta cttgatggaa aaagttgtca aaactgaacg
     1321 tgctgttgct gtggtaaaga ctattgatga aaggtgtaac ttcaagctgg agaccattct
     1381 catctgtcaa tttataatcg atcaggaatt gctggaagct ttctaaatgg atataaatca
     1441 cattttttcc tttagcgagc ccgaacaaat catcatttgg tgctgcataa tgttctttta
     1501 cataattttc tacttctttc atatcattgg gacttgcttc tgcccgcact tggtttgtct
     1561 ggtaagtttg aacaccgtca taaaccatga aagcattcaa gccaagatat ttgaccaaat
     1621 aatcacgaga gaatgttcga gtcagcaatt ctggacgatc agcttccgcc ataaaaagat
     1681 ttcctgagaa aatcataact gctaatgtag agatcgcaaa agccattctt gctcggatcg
     1741 ggcgagggtc caacttgatt ttttttgcaa tcagcagaac agtcaagata ataaaatcga
     1801 tccaatagat cacatcatat gggcgaaaca gtcgaatggc actttctccc agaccactcg
     1861 ctactttccc agcacctaaa atcgtgttga tcgtaatgaa gtccgtgaat tctcgataat
     1921 aagtgacatt cgaaaaaaca agcaatgtca aaagaaaata aatgaccatc atcgtgatat
     1981 aagcaactct agggcgtctg atataaagag caattccaag taataacaat gtagttgcaa
     2041 tagggttgat aaacaagata aagtattgaa tgccactttc gattccgaga tggaaatcaa
     2101 ctaaataggc aaaaatcgtt ttcaaccaaa gtaaaacaac tagtaaagaa aaaaatccga
     2161 gcctcttatt caaaaaggca ggtgttttta tacatttcaa gataatcttc ctttctgcac
     2221 ttcttaatca cagaagtgtc atgaaaccat gttacacagc tcacttattc tcatttattt
     2281 tactagtatg acaatatagt gtcaattgaa accagaagtt tatgctgttt cattaaggtt
     2341 ttctttaagc ttcatgaaaa aatgagcagt aagacaagtc ctatgttttt tgttatactt
     2401 gggaagagaa agctaaagca ggtgaaggaa tgaaagtaaa agtaaaaaaa agagcaagca
     2461 atcgattgaa aaaaggatat ccactgatcc aaaaagaaga ccttgctcat ccccaaaaag
     2521 aaaccacgca atgggtggaa ttagttgatt cgcaaaacca atttatcgca aaaggatatc
     2581 taggaaagca gaataaggga atcggttggg tattgagcca gcagaacatc ccatttgatc
     2641 aatctttttt tgaagaattt tttgtgaagt caaaagaaaa aagaactttc tttttttcag
     2701 atgattctac taccgctttt cgtatattca atggagaagg agacgggatc ggcggtctga
     2761 ttgttgatta ttatgcaggt tatgctgttt tttcttggta taacgaaacg ttatatgcac
     2821 atcgtgaatt attgattcaa gcttttcgtg aaatctaccc ggaaatcgta ggaggctatg
     2881 agaaaattcg atttgaatca gtaaatctgc ctgagagcca gttcatttac ggaaaagagg
     2941 caccggaacc cttgatcgta aaagaaaacg gagtaaaatt tgccgtttat ttaaatgaag
     3001 gattaatgac tgggatcttt ttagaccaaa aagaagtacg gggatcatta gcagaaggtt
     3061 tcgcttctgg gaaaacagtt ttgaacatgt ttagttatac cggcgccttt tctgttgctg
     3121 ccgcaatggg aggggcaatc gaaacaacaa gtgtcgatct agcaaaacgc agtttaaaaa
     3181 aaacacagga acagtttgaa gtgaatggtt tggatgtcga tcagcaaaaa atcattgtta
     3241 tggacgtatt cgagtatttc aaatacgcca aaagaaaaca atttttatat gacatgattg
     3301 tattggatcc accaagtttt gccagaaata agaaaaaagt ctttcgcgta gcaaaagatt
     3361 atggggaact ggtcaaggat agtctggata ttttagcaga agacgggata cttattgcgt
     3421 ctacaaatgc tgcgaatgtc tcgatagaac aattccaaac aatgattgaa gaggaattcc
     3481 atgccaaaaa tgtttctttt gaacaacttg ctttatatcg tctgccaaaa gatttccaga
     3541 cgacggatac ctttttagaa ggcaattatc tgaaagtgtt tgtttatcaa attaaaaata
     3601 accattagaa ggagcgatgc agaatggttt gggtaaaggg attgaatata ggaagcggta
     3661 gaccaaagat ttgtgcgcct cttatcgggg ccgtccgaga ggaagtatta cgtgaagcag
     3721 ttcttgcacg tgaagcaggg gccgatttag tcgaatggcg agtcgatcat tatcgtgaag
     3781 ttttttcttt tgatgaagta gcagaaacat tggttcttat ccatgagatg cttgatggac
     3841 tgccgttatt atttacgttt cgtaccttag ctgaaggcgg agaacaagac atatcgatcc
     3901 gtcagtatcg cgacctttat gaacatgtga tacacacagg attagtagat atggtcgata
     3961 tcgagttgtt caaagtagaa agtctaggaa aaagactgat agaggaaatc aagtctttaa
     4021 acattccttt aattatcagt agtcatgatt ttaaagaaac accagcagac cctgtcttgc
     4081 tttaccgatt gaatgttatg gaacattttg gagccgatat cggaaaactg gcagtaactc
     4141 ccaataatga acgagatgtt ttgcggttga tggagttgac tagacgggca aatgcttttg
     4201 tctccatgcc tattattacg atgtccatgg gaaatcttgg taaaatttct cgtctggctg
     4261 gcggaacgac gggttccgtt atgacatttg gttccttaag cagcgattct acttctgctc
     4321 caggacaatt accggtcaaa gcattgaagc aaattattca attattaaat gaaccgttgt
     4381 agaacaagtt tgaggtgata ttttttggca aagaaaaaaa tagtaaaaac gaatgcaatc
     4441 cgtatggtcg aacaaaaaaa gattccctat acagagcacg aatatgagtg ggacgaaagc
     4501 cacctaagtg cgtcatcggt cgctgaacag cttccagaaa gccaatctca tatatttaaa
     4561 acactggttg ctgtgggcaa tgtgacgggg cctcttgttg ctgtgatacc gggagaagcc
     4621 gaattgaatc taaaaaaatt agcaaaagtt tcagggaata aaaaagtaga aatgctccat
     4681 ttaaaagatt tggaagcaac gaccggatac atacgtggtg gctgctcgcc tataggtatg
     4741 aaaaaactat ttcctactta tttagatcaa attgcagaaa gctatgaaca aatcattgtc
     4801 tcggctggca gaagagggct acagatggag ttagcacctc aagatatctg tgcattaacg
     4861 agcggacaat ttgcagatat aaaacaataa atcatacgta taaaagaaag aaggaggccg
     4921 ggatgttgtt tgtccagtct ccttctttct tttttataca ttatgctctt tgcccggcat
     4981 tcattcctgc tacatgccct gtagcgaaag ccgctgtgat attaaagccg ccagtataac
     5041 cattgacatc cagtaattct cccgcaaaat acagaccatc aatgatttta ctctccaatg
     5101 ttttaggata aatttccttt agagaaaccc ctccgccagt tacaaatgat ttctcaatag
     5161 gaaaagtttt gttgattgat agcttaaact ctttgcatag ttgaacaaaa tcttgaattt
     5221 gtttttgaga ggcttgttgt cctgtcagat gatccatttc aagccgctca agaaagaaaa
     5281 ctaacaatcg ctcaggcaat ataccatgcc aagcattgac caagttcttt ttggttgctt
     5341 tacttttttc tgtcaatata tgaataagct cttgtttggt ttgtgtaggg aaacaatcca
     5401 gagaaagtgt cactggttct cccgttttct ctaattcttt attgacaaaa cttgaacaac
     5461 gcagtgcagc cggaccagaa ataccgaaat gggtgaaaag taaatccatc gtatgttcag
     5521 tcaatacacg acctttttta tttaaaacgc gtaaagtgat gtcttgtaaa gaaagccctt
     5581 gaagtgtttt ctcctgaata tacggttcat ccgatatcaa gggggactct gtcgcataaa
     5641 gaggcgtcac cgtgtgaccc actcgcttca cgatcttgta cccatctcca gtagaaccag
     5701 tagatggata agtccgtcca ccagttgtta agataatggc aggagcatgg aactcttcga
     5761 attccgtgcg tataccgtaa attttatgtt ctttgtggat caatttctct actcgagtag
     5821 agaatagaag cgtcacatct agttctttca gacgattgag caatgcttcg ataatcgttt
     5881 ttgatttatt tgttacagga aacatccgtc catgatcctc ttcttttaaa tgtacgcctt
     5941 gtgattcaaa gaagttcatg atatcgaaat tattccattg cgcaaacgta ctatataaaa
     6001 actttccatt tccaggaata tgtgcaatca aatcatcaac aggacgattg ttcgtaacat
     6061 tgcaccgacc accacctgtc atcagtagtt tttttcctgc ttttttgttt ttttcgatca
     6121 agagtacgcg tgcgccttgt tcagctgcac tgatcgctgc catcatcccg cttgttccag
     6181 cgccgactac gatcacatca tacagttttt ccattccctc acctcaagga aagtgtagca
     6241 tatctgaaga aagaaaacag aaaaaatgtt ttgagccaaa agagaaaatg agctaaaatg
     6301 agggaagata agcaaaggag cgtggaacga tgacaaacgc aacagaatat gttcaagcaa
     6361 ttcaagaaaa gctacatcaa aaggatcaag ggcaagaaga atttttacaa gcaatcgatg
     6421 aattcatgcc cacggttatg tcttttttgg acactcatcc cgaatatatc gaaaagaata
     6481 tcttagaact tttaacggaa ccagaaagag ttattcaatt tcgagtgcct tggcaagatg
     6541 atgaaggaaa ctggcgagta aatagagggt accgtatcca atacaactca gctatcggcc
     6601 cttataaagg tggattgcgc ttccatccta gtgtcaatct aagtatcttg aaatttttgg
     6661 cttttgaaca gattttcaag aatagtttaa ccggtctgcc aatcggtgga ggaaaaggcg
     6721 gaagtgactt cgatcctaaa ggaaaatctg ataatgagat catgcgtttt tgtcaaagtt
     6781 tcatgctgga attgtcaaaa catatcggcc catcgattga tgttcctgcg ggagatatcg
     6841 gtgtaggagc tagagaaatc ggctatttgt tcggcgaata taaacgattg aaaaaatatg
     6901 atactggtgt gttgacaggt aaacctttgg atttttgggg aagtaagata cgtacggaag
     6961 ctactggtta tggattagtg tattatgtca aacatctatt gaacgaagaa aaagattcat
     7021 ttaatcaaaa aacagttttt gtttccggaa gcgggaatgt agccatttat gcaatcgaaa
     7081 aagtgcaaga gctaggcggg aaagttgtta catgttcaga ttctagcggg tacatctatg
     7141 atccagaagg aatagatgtg aaactattga aagaagtcaa agaaatcaat agacaacgtt
     7201 tgacggaata cgcaaaccaa cgtccttctg ctatttatca tgctggagaa tctgtttgga
     7261 cattgaaaga aaaagcggat atcgctttgc catgtgctac tcaaaatgaa atagacgaaa
     7321 gcttggccga aatcttagta gaaaatggga tcaaaatcgt agcagaaggc gctaatatgc
     7381 catgtacagt aaaagctgtt tctgttcttc atgaagcagg cgtgtggtat tgtccaggaa
     7441 aagctgctaa tgctggagga gtagcagtat ctgctttaga aatgagtcag aacgcccagc
     7501 gattgacttg ggaaagagag caagtggatg agcagctgga tcaaatcatg gaaacgattt
     7561 accatacgtg tcgccaaaca gcagccgagt acggcgacga aaaaaatctg cttcttggag
     7621 caaatgttgc tggatttgaa aaagttgcca aagcaatggc agcagaaggt ttagtttgaa
     7681 aaatgaaact ttcttggaaa tattgtcagc attttgttta tttgcttgaa aaataataaa
     7741 aaaaaacgta aagtattacc tgtagagtaa acctataagg aggagaacaa atggcacaca
     7801 ttcattttga ctattcaaaa gtagcaccgt ttgtcaacga acaagagtta gggtacatgc
     7861 aaagccaagt aacagcagcc cacaacgaat tacgggaagg tactggcgca ggaagcgact
     7921 tccgcggctg gatcgattta ccaacgaact atgacaaaga agaatttgca cgtatcaaag
     7981 aagcagctaa aaaaatccaa tctgattctg aagtattagt tgttatcggt atcggtggtt
     8041 cttatttagg cgctcgtgca gcgatcgact tcttgaatca cacattcttc aatttattag
     8101 atagcaacac aagaaaagca ccacaaatct tctttgccgg aaattcaatc agctcaacat
     8161 atattgctga cttgatcgaa gtaatcggtg atcgcgactt ttcagtaaac gttatttcaa
     8221 aatctggaac aactactgaa ccagcgattg cgttccgtgt attcaaagag cttcttgtta
     8281 agaaatatgg acaagaagaa gcaaacaaac gtatctacgc tacaacagat aaagcaaaag
     8341 gtgcagttaa agtagaagca gatgcagaag gttgggaaac tttcgtgatt cctgatgaca
     8401 tcggcggacg tttctctgta ttaactgctg ttggtttgtt accaatcgct gttagtggtg
     8461 cagatatcga tgcattgatg caaggtgccg ctgatgcaag caaagcatac tcaagcgaca
     8521 aactagaaga aaacgaagct taccaatacg cagcaatgcg caacattctt taccgtaaag
     8581 gcaaagtaac tgagttgttg atcaactatg aaccaggaat gcaatacttc tcagaatggt
     8641 ggaaacaatt gtatggtgaa tctgaaggaa aagaccaaaa aggcatctat ccttcaagcg
     8701 caaacttctc aactgatttg cactctttag gacaattcat ccaagaagga cgtcgcaata
     8761 tttttgaaac agttgtgaag gttgaaaaac ctcgtaaatc aatcacaatt cctgaacaag
     8821 cagaagattt agacggtcta ggctacttgc aaggaaaaga agtggacttc gtaaatacaa
     8881 aagcatttga aggaacattg cttgctcata cagacggtga cgtgcctaac ttgctagtca
     8941 aaattccaga aatggatgct tatacattgg gttatacaat gtacttcttt gaaattgctg
     9001 taggtatttc aggttacttg aatggaatca acccattcga ccaaccaggt gtagaagctt
     9061 acaagaaaaa catgttcgca cttctaggca aaccgggctt tgaagatctt gctaaagaat
     9121 tgaacgaacg cctgtaagat aagggttttt cgctagattt aaataatcag tgatagaaac
     9181 atgttcgctt taaaatgaaa actggtaccc atttaggtac tcaaacatac tgcatcgttt
     9241 attcagaata aggggctgtg acaagacgtt tgtcacagtc ccttgtttgt atagaaatcg
     9301 attgattgcc ttgctcttta gtttgtttta ggactttaag aacagtgagg ctgacttcga
     9361 aggttaattc tgtttttttg ttttctagaa tcagtaagca aaaaagcact cacttcgagt
     9421 gcttttttgc gatatattct tagcttaaga taggcctaat ttttctttta cagatgaggg
     9481 taccttatcg ctggtaatta cttttttctc tatgatccgc ttttcgcttg ctttcaactg
     9541 caagatcgtt ccttcggtgt aagcagaacc attggcagag tcaccagaag taatgacatc
     9601 gaaatctaac tgtctttttt caccgttttc agcataagca tctacgttgt atttaaaacc
     9661 tcttccaatt acttccccat tatctgcttt gatatcagtt tctgcaggca ttggagcttt
     9721 gactaccgca taataatctt tacctacata agtagactga tagtagtccc aacctttcca
     9781 tcctataaaa gcaatcaata tgataa
//
LOCUS       DAZKLD010000110         9792 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.110, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000110 DAZKLD010000000
VERSION     DAZKLD010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9792)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9792)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9792
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.110"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(180..1028)
                     /locus_tag="ACG8KR_002228"
     CDS             complement(180..1028)
                     /locus_tag="ACG8KR_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFQ0201557.1"
                     /translation="MSVSIRLQIKTMYNDFSPKEQAIADYILENPSKVSHSPISNLAS
                     ELGIADSTFFQFTKKLGYNGFKDFKMAMLIQENDLSAISIHENIQKTDTELTMAQKVF
                     ESSISTLNDTKKLLQQKDLKKAATIINESKRVYFFGVGGSEIVATDAYHKFLRSPIAT
                     SHSTDYHIQLMEASLLTEKDCAVLISHTGQSKETIHIAETVRKTGAKTIVITSQANSS
                     LAKLGDVVFISISEETEFRSEALASRISQLSILDSLYVILMFYNEKESRRSIAKVRHT
                     ISEIKE"
     gene            1192..2094
                     /gene="gnd"
                     /locus_tag="ACG8KR_002229"
     CDS             1192..2094
                     /gene="gnd"
                     /locus_tag="ACG8KR_002229"
                     /EC_number="1.1.1.343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydrogenase
                     (NAD(+)-dependent, decarboxylating)"
                     /protein_id="HFQ0201558.1"
                     /translation="MEIGVIGLGKMGLNLALNLKEHHHKVQGLDISSTAVSQARENDI
                     PAYQEISEFLATFEQKKIIWLMLPAGEITENMLRKLFPKLGPGDIIIEGGNSYYKDSI
                     RRAEEFQKNEIGYLDCGTSGGIEGARHQACLMIGGDKETFLSVEQLFKDVAIERGYLY
                     AGASGSGHFLKMIHNGIEYGMMQAIGEGFQLVQKSEYAFDLEQVARVWNHGSVIRGWL
                     MEIMEDQLNEHPNLSNYRGIVSASGEAKWTVETALEMAVPVPVIATSLFMRNLSQEED
                     SFSAKVVSALRNGFGGHEIVEKGE"
     gene            2096..3097
                     /locus_tag="ACG8KR_002230"
     CDS             2096..3097
                     /locus_tag="ACG8KR_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-isomer specific 2-hydroxyacid dehydrogenase
                     family protein"
                     /protein_id="HFQ0201559.1"
                     /translation="MPDYHIAIVNSSSFGRVFPHHLERLKSVGDVHFFTVDQEIPGKE
                     LAELLKGFNIIIASVTPFFRRDFFENKDELLLISRHGIGYNNIDLKAAKEHQTIVSII
                     PALVERNAVAENNITNLLSMLRRTTQAQARVKDDRWEDRAEFVGRTLFNKTVGVIGAG
                     NTGSGVIEILRNGFRCDVLAYDPYKSDLYIESFGAKKVELQELLEQSDIICLCANLTE
                     ENYHMISDKEVDQMKENVYLSNSARGALVDEEAMINGLKSGKIAGYATDVLEEEPGRQ
                     DHPYLAFEQVVITPHISAYTRECLEAMGEKCVRDVEQISQHLLPERSVQTKSNYIKG"
     gene            3094..3378
                     /locus_tag="ACG8KR_002231"
     CDS             3094..3378
                     /locus_tag="ACG8KR_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HFQ0201560.1"
                     /translation="MMKKMVNGLKVKTGPQFYLYEEGGISKVSDLLKSYGAKRVLVTH
                     GTVSWEKALPKLVFLNDETIQFFYHRYSGECSYAEARRIATIIKKMKSIS"
     gene            3411..4208
                     /locus_tag="ACG8KR_002232"
     CDS             3411..4208
                     /locus_tag="ACG8KR_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HFQ0201561.1"
                     /translation="MGYSANLARIDFGVIPTLASNCAPWTPLAVMYKENGQAEGKTEH
                     FYRQAAFFITDPQLVIDSPVPYFIAGLADTLAKWYESELILEQDFLQDESFLKLAQDT
                     AKICKEEILNHSAKAIQDMAQRKLTSEFKQLSEIIFAVAGLVGGLGDKYARNVAAHAM
                     HDAISKYIPESHRFLHGEKVAYGIFYQLALEGRWEKIDELYPLYRELELPVSLEQMGL
                     FPKDKQIIDKMAEFIDSKEKVHLIPIEVTQKSLIQSMMELEGYIRKI"
     gene            4222..5055
                     /locus_tag="ACG8KR_002233"
     CDS             4222..5055
                     /locus_tag="ACG8KR_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFQ0201562.1"
                     /translation="METLSVVQSLLIALWVAAVMSRWLGGGATLTLRFSPLMTGLVVG
                     LIMGEVAQAMIVTAALQMIYMGVFSPGGSMPAEPSIAAAIAVPVALLGDLSPEAAIAV
                     AVPVGLLGSYLYQFRFFINTFLGKYTDKAVEDLNTKAMARSIILYPTVASFLLFVPLV
                     FIALYLGAPLIADVIKALEGTVVIHILEVVGGGLAAIGIATTVYVIGRKDYLVFFFLA
                     YFMSVVLKSLDITMVTYAIFGIIIALIFVQSQKGKQVQPATSNNNSASFDDEDDYDDG
                     F"
     gene            5073..5930
                     /locus_tag="ACG8KR_002234"
     CDS             5073..5930
                     /locus_tag="ACG8KR_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFQ0201563.1"
                     /translation="MAEIMDNQTGAPEEITKKDVTKAYLRWHFANEIPHSFERYLAPS
                     LLYGMMPILKKLYKDEDSLKAAYKRQLLFFNTQLSWGGGVITGLMSSMEQQRAEEEYE
                     EKEILMQDDLMYNTKAGLMGALAGIGDAIDSGTVQYIFIAIAVPWAQQGSPLGALFPF
                     ICFGLYQVLLGVFFARQAFTMGRNATGLKQNTGVQTAIEMLSVLGLFMMGVLAGNYVK
                     VESSLKFAISGREFVIQEILDQIVPGILPLAVVMGVYLFYTKKGLKVTQALLWLTGIL
                     IVLAGIGIL"
     gene            5952..6461
                     /locus_tag="ACG8KR_002235"
     CDS             5952..6461
                     /locus_tag="ACG8KR_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HFQ0201564.1"
                     /translation="MGVVNLARVDERLIHGQVMVTLSQKSGVNSIFVVDEVVAKDKFM
                     RDLYKNAGSRTGQKTIVITPEKAKFYWDEYQFKEYNCILIAKTVQTIYDLVKHGIPME
                     ELNIGGIAQKNPDTDLLVTKSVYLNKSDAEKLKEMNEQYGVKNIYFQATPSAPKTGLK
                     DVLNKFDLS"
     gene            6498..6899
                     /locus_tag="ACG8KR_002236"
     CDS             6498..6899
                     /locus_tag="ACG8KR_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0201565.1"
                     /translation="MKKNELFRDWEFRYRYIYRKRRTKKSKQRFLSALVSDIYSMRTD
                     VTVIAYDTLAYRSKNIYVGDIEKAEKVICTYYDTPVHALGSYFMFDWKDQRKKTIYSI
                     LLSFILLFSLGWWGMMIYNKNPHHVFDLLSV"
     gene            7011..7364
                     /locus_tag="ACG8KR_002237"
     CDS             7011..7364
                     /locus_tag="ACG8KR_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0201566.1"
                     /translation="MLEMIRTIDDPSVAYAFVDEGCYGEKGLDSVRSGMKKEAILFYL
                     DSVGADTPLQFSGNYFSNKEQWLKQVDKLKEKNVNYIFSARKKQAQFFYLTKTDLRGK
                     TFNWQNANQIIALFR"
     gene            7398..7841
                     /locus_tag="ACG8KR_002238"
     CDS             7398..7841
                     /locus_tag="ACG8KR_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFQ0201567.1"
                     /translation="MLEIMIVTHGKLSEGLKDAAEVIVGTSSHIETMCLESGEDIQTF
                     GEKVKEKLTMINTAEPMLVFVDLISASPYNQALLAVNELTEEKKAATYVIGGVNLPML
                     LEAINHQMLHTPIEEAVEQIVKQGKDNLTVWHASAEEKKLDEDDF"
     gene            7858..8943
                     /gene="uxuA"
                     /locus_tag="ACG8KR_002239"
     CDS             7858..8943
                     /gene="uxuA"
                     /locus_tag="ACG8KR_002239"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFQ0201568.1"
                     /translation="MKWGFRWYGEKGDSIPLKYVRQIPGINGIIGTLLTKMPGDVWEI
                     SEIEELKASVESQGLELLGIESVAIHDAIKAGTDERDYYIDNYIRTIKNLSRCGVRLI
                     CYSFKPIFGWAKTDLFNENNDGSYSLLYDQKIVDQMEPGEMYQLIHSQSKGFKLPGWE
                     EERLKKFAQLEKLYEGMTEEELFENLTYFLEKVIPVCEKADVKMAIHPDDPPWELFGF
                     PRVTKNLGDLKKILNAVDSPYNGLTLCTGSLGANPENDLVEIIHEVGSRIHFVHFRNV
                     EFLGERKFKETAHPSTEGSLDMYHIMKALVQEGFDGPIRPDHGRTVWGEVAMPGYGLY
                     DRAMGLTYMQGLYEAISKEKPLGEHYE"
     gene            8936..9649
                     /locus_tag="ACG8KR_002240"
     CDS             8936..9649
                     /locus_tag="ACG8KR_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFQ0201569.1"
                     /translation="MNKIKLFIFDMDGLLLETGRLAYRAYVKSAQKYDYEMRKEVYYL
                     LTGQTEMAIREQMGILYGEDVPYIKWREAINQYKEKIVKEDKRVYTKKGAEEILSFAK
                     ERGIHTIVASSNTRVKVEMYLKMENLYALFDQIISGDDVKKGKPEPEIFLKACSKMNI
                     PPSEALVFEDSIAGIEAARRAGILSFLIKDHLIGLPDHNGKHKLKVDVSLYESNKADY
                     EFYDLHQAKRFLESKGLYL"
BASE COUNT         3255 a         1551 c         2165 g         2821 t
ORIGIN      
        1 atctaacaag taactcgaat aataccaata caaccttctc caaagatgcg atgacatacg
       61 ttctaaccca ccgactattg atgttctgta ccacccttgc agaaccaaaa aagccactac
      121 caaatatttg gtagtggctt tttttgtttt atggattttt cgctttttct gttatggtgc
      181 tattctttga tttcagaaat agtatgtcga actttagcga tagaacgacg tgattctttt
      241 tcgttgtaaa acatcaaaat cacatataga gagtccaaaa tgcttagttg agaaatacgg
      301 gaagctaaag cttcagatcg gaattctgtt tcttcagaaa tagaaataaa aacgacatcg
      361 ccaagtttag caagggaaga attagcttgg ctagtaataa cgattgtttt tgctccagtt
      421 tttcttacgg tttcagcaat atggattgtt tcttttgatt gccctgtgtg agaaatcaaa
      481 acagcacagt ctttttccgt caataaagaa gcttccatca attgaatatg gtaatccgtg
      541 ctgtgagaag ttgcgatagg ggaacgcaaa aatttatgat aagcgtcagt tgctacaatc
      601 tctgaaccgc caacaccgaa aaaatagaca cgtttagatt cattgataat agtagccgct
      661 ttttttagat ccttttgttg taacaacttt tttgtgtcat ttaaagttga aatactagac
      721 tcgaacacct tttgcgccat agtgagctca gtatctgttt tctgaatatt ttcgtggata
      781 gaaatagcag atagatcatt ttcttggatc aacatggcca ttttaaaatc tttaaatcca
      841 ttataaccaa gtttttttgt aaattgaaaa aaagtagagt cagcaatgcc cagttcactt
      901 gctaaattgc taataggact atgacttact ttgcttggat tttctaaaat ataatcggcg
      961 atagcctgtt cttttgggct gaagtcgttg tacattgttt tgatttgtag tcttatggag
     1021 acgctcatga gaattcctca ttttcataat aagattatca ttttttccgt tctcattaat
     1081 gttaaagata caaggagagt aggaaaaaat caaacaaaaa atatttttgt aaaatctatt
     1141 tacaaaaaaa tttttttgta taatgagggt ataagaaatg gaggcgctat catggaaatt
     1201 ggtgtaattg gtttgggaaa aatgggattg aatttagctt taaacttaaa agagcaccat
     1261 cacaaagtac aaggactcga catttcaagt acagctgtta gtcaggcaag agaaaatgac
     1321 attcctgctt atcaagagat ctctgaattt ctagctactt ttgagcagaa aaaaatcatc
     1381 tggttaatgc ttcctgctgg agagataacc gaaaacatgt taagaaaact ttttccaaaa
     1441 ctcggaccag gagacattat tattgaaggc ggaaactctt attataaaga ttcaatccgt
     1501 cgagcagaag agtttcagaa aaatgaaatc ggttacttgg attgtgggac ttctggagga
     1561 atcgaaggag cacgacatca agcctgctta atgattggag gagataaaga aacttttcta
     1621 tccgtggaac aattattcaa agatgtagca attgaaagag gatatctgta tgctggtgca
     1681 tccggaagcg gacacttctt gaaaatgatc cataatggaa tcgaatatgg aatgatgcaa
     1741 gcaatcggtg aaggattcca acttgtacaa aaaagtgagt atgcttttga cttggaacaa
     1801 gtcgcacgtg tttggaatca tggttcggtg attcgcggct ggctgatgga aatcatggaa
     1861 gatcagttaa acgaacatcc caatttaagt aattatcggg gaatcgtttc tgcatcaggt
     1921 gaagcaaaat ggacggttga aacagcgcta gaaatggctg ttccagttcc agtaattgca
     1981 actagtttgt tcatgaggaa tctttcacag gaagaagata gtttttcagc aaaagtagtt
     2041 tcagcactaa gaaatgggtt tggcggacat gaaatagttg aaaagggtga gtaaaatgcc
     2101 agattatcat atagcaatcg ttaattccag cagtttcgga agagtctttc cacaccactt
     2161 ggaaagatta aagtcagttg gagacgttca tttctttact gttgatcaag agattccagg
     2221 aaaagaacta gcggagttac ttaaaggatt caatatcatt attgcaagtg ttacgccatt
     2281 ttttcgacga gatttttttg aaaacaaaga tgaactgcta ttgatttccc gacatggtat
     2341 tggttacaac aatattgatt taaaagcagc aaaagaacac cagacaatcg tatctatcat
     2401 acctgcttta gttgaacgaa atgctgtagc agaaaataat ataactaatt tgttgtccat
     2461 gcttcgacgc acgacacaag cacaagcaag agtcaaggac gatcgatggg aagatcgggc
     2521 ggagttcgtc ggtaggacgc tgtttaacaa aacagttggt gtgattggtg caggaaatac
     2581 cggaagcgga gtaatcgaaa ttttgcgtaa cggttttcgc tgtgatgttc tagcttatga
     2641 tccttacaaa tcagatttat acatcgaaag ctttggtgca aaaaaagtag aacttcaaga
     2701 attactggaa caatcagata tcatttgttt atgtgcgaat ttgacagaag agaactatca
     2761 catgatttct gataaagaag tcgatcagat gaaagaaaat gtttatctat ctaatagtgc
     2821 aagaggcgct ctagttgatg aagaagcgat gattaatggt ctgaaaagcg gaaagatcgc
     2881 aggatatgcc acagatgttt tagaagaaga accgggacgt caagatcatc cgtatttagc
     2941 gtttgaacaa gtagtgatta cgccacatat atcggcctac acaagggaat gtttagaagc
     3001 aatgggtgaa aaatgtgtca gagatgtcga acagatcagt cagcaccttc ttcctgaacg
     3061 ttctgtccag acaaagagca actatataaa aggatgatga agaaaatggt gaacggttta
     3121 aaagtgaaaa caggccctca attttattta tatgaagaag gtggtatttc aaaagtttct
     3181 gatctcttga aatcatacgg cgcgaaaaga gtactggtca cccacggaac ggtatcatgg
     3241 gaaaaggcgc tacctaaatt agtatttttg aatgacgaaa cgattcaatt tttttatcat
     3301 cgttattctg gagaatgtag ttatgctgaa gccagacgaa tagctacaat catcaaaaaa
     3361 atgaaatcga tttcgtagtt ggcgttggcg gaggaaaact gaccgatcta gtgggttata
     3421 gtgccaatct tgcaagaatc gatttcggag tcatccctac tttagctagt aattgtgctc
     3481 catggacacc tcttgctgtc atgtataagg agaatggaca agcagaaggg aaaacagaac
     3541 acttttacag acaagctgct ttttttataa cagatcccca attagtgatt gattcccctg
     3601 ttccatattt cattgccggg ttagcagata cactagcaaa atggtatgaa tctgagctca
     3661 tcttagaaca agattttttg caagatgaat catttttgaa gctagcacaa gatactgcga
     3721 agatctgcaa agaagagatt ttgaatcatt ctgctaaagc aatccaagat atggcacaaa
     3781 gaaaactgac atcggagttc aaacagcttt cagagattat ctttgctgtt gctggtttag
     3841 ttggaggatt aggagataaa tacgcaagaa atgtggcagc acatgctatg catgacgcta
     3901 tcagtaaata tattccggaa agccatcgtt ttctacatgg agaaaaagta gcgtatggga
     3961 ttttctatca actggcacta gaaggtcgct gggaaaaaat agacgagtta tatcctttgt
     4021 atcgggaatt agaactgccg gtttctctag aacaaatggg attatttccg aaagataaac
     4081 agataataga taaaatggct gaatttattg attcgaaaga gaaagtgcat ttgattccta
     4141 tagaggtaac acagaaatca ctgatacagt caatgatgga actggaaggt tatatcagaa
     4201 agatctgaag gaggaggaca catggaaaca ttaagtgtag tacaaagttt gttgattgct
     4261 ttatgggtag ccgcggttat gtcccgctgg ttaggtggag gagccacgtt gaccttacgt
     4321 ttttctcctt tgatgactgg attagtcgtc gggttgatca tgggggaagt tgcacaagcg
     4381 atgattgtta cagccgcttt gcaaatgatt tatatggggg ttttttctcc aggaggatca
     4441 atgccagctg aaccatctat tgcagcagct atcgcagtac cagtcgcatt attaggggat
     4501 ctgtcgccag aagcagcgat tgccgttgcg gtgccagtcg gattgctcgg aagttacttg
     4561 tatcagtttc gttttttcat caatactttt ttaggtaaat atacagacaa agctgtagaa
     4621 gatttgaata caaaagctat ggcccgatca attattcttt atccaacggt tgcctcattt
     4681 ttattattcg ttccgcttgt tttcattgct ttatatttag gggcgccatt gatcgctgat
     4741 gtaatcaaag ctttagaggg aacagtcgtt atccacattc ttgaagtagt aggtggaggg
     4801 ttagcggcaa tcggtatcgc gacaactgta tacgttatcg gtcgaaaaga ttatctggtc
     4861 tttttcttcc ttgcttattt tatgagtgtt gttttgaaat ctttagatat tacgatggtt
     4921 acttatgcca tttttgggat tattatcgct ttgatctttg tccagtcgca aaaagggaaa
     4981 caagtacagc ctgccacaag caataacaat tcagcatctt ttgatgacga agatgactat
     5041 gatgacggat tttaatttag gaggaatggg aaatggctga aataatggat aatcaaacag
     5101 gtgctcctga agaaatcacg aaaaaagatg taacaaaagc ctatctgaga tggcattttg
     5161 caaacgaaat tcctcactct tttgaacgtt acttggcgcc atcactacta tacggaatga
     5221 tgccaatctt gaaaaaactt tataaagatg aagactcttt aaaagcagca tataaacggc
     5281 agttactgtt cttcaacact caattaagct ggggtggcgg tgtgattact ggcctaatgt
     5341 cttctatgga acagcaaaga gcagaagaag agtatgaaga aaaagagatc ttgatgcagg
     5401 atgacttgat gtataacacg aaagcgggat tgatgggggc actagcagga atcggagatg
     5461 caatcgattc aggaaccgtc caatatattt ttatcgcaat cgcggtacct tgggcacaac
     5521 agggaagtcc tttaggcgca ctttttcctt ttatctgctt tgggttgtat caggttttac
     5581 taggggtatt tttcgcaaga caagctttca ctatgggccg taatgctaca ggcctaaagc
     5641 aaaacactgg tgttcagaca gcgattgaaa tgctatcagt tttagggttg tttatgatgg
     5701 gggtgcttgc gggaaactat gtaaaagtag aatcttcatt gaaatttgcg atatccggac
     5761 gtgagtttgt tatccaggaa attcttgatc aaatcgtgcc agggatatta cctttagctg
     5821 tcgtaatggg tgtgtacttg ttctatacga aaaaaggatt gaaagtcaca caagcattat
     5881 tatggctgac tggaatatta attgtgctag caggaatcgg tattttataa aaagaaggga
     5941 acgtgaataa gatgggtgta gtcaatttag cgcgtgtgga tgagcgtttg atccatgggc
     6001 aagtaatggt aacgttatct caaaaaagtg gagtgaattc catttttgtt gtagacgaag
     6061 ttgtagcaaa agataaattt atgagagatc tttacaaaaa tgcaggaagc cgtacaggac
     6121 aaaaaacaat tgtcattaca ccagagaaag ctaaattcta ttgggatgaa tatcaattta
     6181 aagaatacaa ttgtattttg atcgcaaaaa cagtccaaac gatttacgac ttagtaaaac
     6241 acggaatccc catggaagaa ttgaatattg gagggattgc tcaaaaaaat cctgatacag
     6301 atcttttagt gacgaaatct gtttatttaa ataaatcaga tgctgaaaaa ttgaaagaaa
     6361 tgaatgaaca atatggcgta aagaatattt attttcaagc cactccatct gcacctaaaa
     6421 caggattaaa agatgtattg aacaagtttg atttgtcata aacaaatgct tagaaaaaag
     6481 ataaagaggg cgaaaaaatg aagaagaacg agctgtttcg tgattgggaa tttcgttatc
     6541 gctatattta ccgtaagcgc cgtacaaaaa agagtaaaca gcgttttctt tcagcattag
     6601 tatcagatat ttacagtatg agaacagatg tgacagtcat tgcttacgat acgctagcct
     6661 accgatcaaa aaatatttat gttggagata tcgaaaaagc agagaaagtt atctgtactt
     6721 attacgatac acccgttcat gcattaggaa gttatttcat gtttgattgg aaagaccaaa
     6781 ggaaaaaaac aatctactct atattattgt ccttcatttt gttgttcagt ttaggttggt
     6841 ggggaatgat gatatataac aaaaatcctc atcacgtatt cgatctgcta tccgtataaa
     6901 caggtatcac tgtactagca tttgggagct actttttctt attgggaaaa gcagctcgcg
     6961 gatggcctag cagacaaact tttattcgaa atacttcatc catattaacg atgctagaaa
     7021 tgatccgtac gattgatgat ccgagtgtag cgtatgcgtt tgttgatgaa ggctgttatg
     7081 gagaaaaggg cttagacagc gtgagatcgg gtatgaaaaa agaggctatt cttttttatc
     7141 tagactctgt tggggcagat acaccgctac agttttcggg aaattatttt tcaaataaag
     7201 aacagtggct gaaacaagtt gataagttga aagaaaaaaa cgtcaattat atttttagtg
     7261 caagaaaaaa gcaagcgcag ttcttctatt tgaccaaaac agatttacga gggaaaacat
     7321 ttaattggca aaatgccaat cagattattg ctctttttcg ataagtaaaa agtgcaaaac
     7381 aaggagtgag aagactaatg ttggaaatca tgatcgttac acatggtaag ttgagcgaag
     7441 gactgaaaga tgcggcggaa gtaattgtcg ggacatcgag ccatattgaa acaatgtgct
     7501 tagagtctgg ggaggacatc caaacattcg gagagaaggt caaagaaaaa ttaactatga
     7561 tcaacacagc agaacccatg ctagtatttg ttgatctaat tagcgcaagt ccgtacaatc
     7621 aagcgctctt ggcagtcaat gaactgactg aagaaaagaa agcagccact tatgtcatag
     7681 ggggcgtgaa tctgccaatg ttgttagaag caatcaatca tcagatgctt cacacaccga
     7741 tagaagaagc ggtggaacaa attgtcaaac aaggaaaaga taatttaact gtttggcatg
     7801 cttcagcgga agaaaaaaaa ttagatgagg acgattttta gaaggaaggc tgaaaagatg
     7861 aagtggggat ttagatggta tggtgaaaaa ggggattcga tcccattaaa atatgttcgc
     7921 caaataccgg gaataaatgg gataattgga actttgctga ctaaaatgcc tggagatgtt
     7981 tgggagattt cagaaattga ggagttgaaa gcatctgttg agagccaagg actggaactg
     8041 cttggaattg aaagtgtagc gatccacgat gcaatcaagg cagggacaga tgaacgagat
     8101 tactatattg ataactatat acgaacaatc aaaaacttgt ctcgatgtgg ggttcgattg
     8161 atttgttata gcttcaaacc aatttttgga tgggcgaaaa ctgatttgtt taatgaaaac
     8221 aacgatggaa gctattctct tttatacgat cagaaaatcg tcgatcagat ggagccagga
     8281 gaaatgtatc aattgataca cagccagtca aagggattca aattgccggg ttgggaagaa
     8341 gaacggttga aaaaattcgc tcagctggaa aaactgtatg aaggaatgac cgaagaagaa
     8401 ttatttgaaa atctgactta ttttttagaa aaagtgattc cagtgtgcga aaaagcagat
     8461 gtaaaaatgg ctatccatcc agatgatccg ccatgggaac tttttggttt tcctagagtg
     8521 acgaaaaatc ttggcgactt gaagaaaatt ctaaatgcag tagattctcc atataatgga
     8581 ctaacgctgt gcacaggatc tttaggggca aatccggaaa acgatttagt tgaaatcatc
     8641 catgaggtag gttcgcgcat ccattttgtc cacttcagaa acgtggagtt tttaggagaa
     8701 agaaagttca aagaaacggc acatccctct acagaaggct cccttgatat gtatcatatt
     8761 atgaaagcgt tggtgcaaga agggttcgac ggtcctatac gccctgatca tggtagaacc
     8821 gtgtggggtg aagtggctat gcctggatat ggcctgtatg atcgtgcaat gggactcact
     8881 tacatgcaag gtctatatga ggcgataagt aaggaaaaac cactgggaga gcattatgaa
     8941 taaaatcaag ctcttcatct ttgatatgga tggcctttta ttagaaactg gacgtttagc
     9001 ttatcgtgct tatgtaaaat cagcacaaaa gtatgattat gaaatgcgta aggaagtcta
     9061 ttatttacta actggccaaa cagaaatggc gataagagaa caaatgggca ttttgtacgg
     9121 agaagatgtt ccatatatca aatggcgaga agcaatcaat caatataaag aaaaaatcgt
     9181 gaaagaagat aagagagtat atacgaaaaa aggagcagaa gaaattttat cttttgcaaa
     9241 agaaagagga atccatacaa tcgtggcttc gtctaataca cgggtaaaag tagaaatgta
     9301 tttaaaaatg gaaaatctat atgccttgtt tgatcaaatc atttcagggg atgatgtaaa
     9361 aaaaggaaag ccggaaccag agatcttttt gaaagcttgt tcaaaaatga atattcctcc
     9421 aagtgaagca cttgtatttg aagactcgat tgcagggatc gaagcagcaa ggcgagcggg
     9481 cattttgtct tttctcatca aagatcattt gatagggttg cccgatcata atgggaagca
     9541 taagttgaaa gtagatgtaa gtttatatga atccaataaa gcagactatg aattttatga
     9601 cctgcatcaa gcaaaacgat ttttagaaag taaagggttg tatctctaat aaagaggact
     9661 attttctagt cctctttatt tattgtcctg cacgactgag ggaacagatg aaaaagaagt
     9721 aattttttat tttgaagtat ctaccttatt tgtagtgaac aatttagaac aaacacaaaa
     9781 cgcatctact gt
//
LOCUS       DAZKLD010000111         9665 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.111, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000111 DAZKLD010000000
VERSION     DAZKLD010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9665)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9665)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9665
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.111"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..632)
                     /locus_tag="ACG8KR_002241"
     CDS             complement(<1..632)
                     /locus_tag="ACG8KR_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFQ0201570.1"
                     /translation="MEIKVLFENSRINLNFKSGHGLSLLINYSGKKYLFDAGANKKLV
                     YNATKMGETLNDLEAVIISHGHSDHTGGLRYLKSGNAPIYTAEGVTLPHYLKILTTFL
                     YVGIPKNEILKMHENLHTIKTKTELAPSFHIIPLGKANSTTKNLYKKMDDKFVYDDFS
                     DELLVVIEQEDGLIVFTGCSHHGIIDMMEIALETFPTKPIKYLIGGFHMIG"
     gene            975..1391
                     /locus_tag="ACG8KR_002242"
     CDS             975..1391
                     /locus_tag="ACG8KR_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFQ0201571.1"
                     /translation="MATIILASHGEFAQGLKQTATMIIGDSVPIHALSAFRDEDESIL
                     VQIKKLLATIDIEETYILTDILGGSVNNDMLTIIKEYEGLQLITGMNLPLVISIATYN
                     GKIGESELEKLIKESQQSVIDCRKLLVEQLTGGDDL"
     gene            1388..1858
                     /locus_tag="ACG8KR_002243"
     CDS             1388..1858
                     /locus_tag="ACG8KR_002243"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFQ0201572.1"
                     /translation="MIKLVRIDHRLLHGQVVFSWSKSLNINRIIIANKEAANDEFKKM
                     SLNLSKPTGIKLTIFSVEQLLEKMPKIETLNENIMLIFGNTKEIRQFCEAYPKVQEIN
                     YGGIIKKEGSKQFSNAIFLNESEQEDAKKLKALGIKQFIQQVPTSKKEDLNTML"
     gene            1872..2645
                     /locus_tag="ACG8KR_002244"
     CDS             1872..2645
                     /locus_tag="ACG8KR_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFQ0201573.1"
                     /translation="MLVQSIGLGLIGVFCILDSRLLGRMNFERPLIVSTLVGLVLGDM
                     EKGLIVGASLELMSLGLVNIGAAAPPDMNMASIIATAFAILSNANAEAALTIAIPISV
                     LGQILGILMRTILSNLTHVADRFIEQGKFSQARRIHILYGSGLYALMYFIPIFLAIYF
                     GTNLVSQIVDAIPEWLTNGLTVASKILPAYGFALLLQTMLTKKMVPFLLLGFLITAYS
                     GLGIIGIAAFACIVAFVMVQVQGKNSNGNQNEPDPLDDL"
     gene            2659..3471
                     /locus_tag="ACG8KR_002245"
     CDS             2659..3471
                     /locus_tag="ACG8KR_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFQ0201574.1"
                     /translation="MKEKDQNAWKYRQFLWRSWAIQASWNYERQMNMGFMYGIAPILD
                     NIYKNPEDEAMKKEAYERHMMYYNCTPQTSAFVLGLTASMEEQYYEDQENFNPESISA
                     VKTSLMGPLSGVGDSFFQGTIRVLAFGLGINLAQQGSILGPILAMLISFVPSFLVTYY
                     GGKIGYNMGNKYLSKLYQEGLMDKVMYVCSIVGLMVIGSMIASMIGITTPIQMGKAFV
                     LQDVLDSIMPQFLPLGLTFFMYWLLQKKVKTGWMLAICLVGGIIFSLLGILQ"
     gene            3525..4490
                     /locus_tag="ACG8KR_002246"
     CDS             3525..4490
                     /locus_tag="ACG8KR_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFQ0201575.1"
                     /translation="MNSKEIVKEIVAKKEVKSIVFVGCGASKADLYPAKYFLDQNAKQ
                     LRISHYTASEFNYATPASVDESTVVISASLGGTTPETVEANAVAKEKGATVISLTRSI
                     DSPLTKDADYVIYHRFAENYAAKLEKTGYCLELAVELLHQVEGYDHYEAMQEGFATIY
                     DLAQKSAESARKDAKKFAERFKDEKVIYVMSSGATHEVAYSTSICLMMEMQWINSGTF
                     HSGEFFHGPFEIVDKDVPFILLMNDGKTRNIDARALTFLERFDTKTEVVDALDYGLSS
                     HIAKEVLDYFNPFVITAVFRVYAEELSEAREHPLTKRRYMWKLTY"
     gene            4606..7329
                     /locus_tag="ACG8KR_002247"
     CDS             4606..7329
                     /locus_tag="ACG8KR_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFQ0201576.1"
                     /translation="MQRLNDYLYQLTLLLKSTPSDNYSARSISQALQLNRSTISSYLN
                     EGVREGLFIKVKSYPVLFLHRTALEELHITLNNSEIESIESLLTISQKPALDQVIGSK
                     GSLKEAIDQIKTAVLYPGKGLPLLLIGASGSGKTFLANKIYEYAVEEKVIHTSAPFID
                     YNCAQYVSNPELLSSALFGYTKGAFTGASQEHTGLLEKADGGVLFLDEVHRLSEEGQE
                     KLFTFMDTGEFSPMGDNSIRKKADVRLVFATTENIYTTFLPTFLRRLPVIVNLPRFQQ
                     RPSFERLSLIDEFFVSESKILAKELSVSDALIHFLMNSDFEGNVGNIKNIIKYACGNA
                     YIHQKSQQTIQVKLLDMPLEYSYKFKEQLNKPRKKRADRIYYPEETRQVQLERNNQLF
                     VQFFDQLLTGFSEVIENNKDIKLFLEEMVTKVTQVMDTLIFQEDYEKEQSLYAILNYH
                     IRQSIDFMRESYGFEQDGNRVVALANYLYVKDNQVIFENSLQEKKTKDQLNEFFANVL
                     ETPYWYAKKILSLLSECLDQPVYEEDIFFITLYFYSLNVTNVKTGIKSIVLAHGYSTA
                     SSMANVANRMLNKNVFQAYDMPLDITLEKIEAKLIQFLGDYRTDSGLILLVDMGSLNQ
                     LGGLLVNHLNGPLLIIDHINTPLLLEVGQQVLNNKSITEIGAEIETSLTFKKQLLLPK
                     KKKKKAIITCCYTGIGSATQIQEILTKCLKDAVRELVIIPYDYKKLLENKNFETPFQL
                     YDVIMIVGTEDPKINQIPYIGLDRLINGEAIGEFVHYLEKELTIEVNDLQKELIFNFS
                     ISKIVENLTILDPDKVLSFVQKAIKELSKLLNFDFSNNQLFLLYLHCSCMIERIVRKE
                     SVDEQEDIEEYMQANGEIMDKIHSAFFEIEREYTIEIPDLELRLLSDIVQG"
     gene            7342..7974
                     /locus_tag="ACG8KR_002248"
     CDS             7342..7974
                     /locus_tag="ACG8KR_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFQ0201577.1"
                     /translation="MTMGVIFDMDGVLIDSETFYFERRMNFFQELEIDPGSNQIDDYV
                     GKTEAAIWETLVPEDKSLREELYPKYVDYRKTHPIDFAKDLRKEVKPLLDFLKKRDIP
                     LALASSSPVVEIESMLIQCELTEYFSFVISGESLKQSKPHPEIYLKAKQQLDCDFFIA
                     VEDSTIGIKSAKAANIYTVALKQPMQIDQSEADIQIESLQELEQIVLEKG"
     gene            8873..>9665
                     /locus_tag="ACG8KR_002249"
     CDS             8873..>9665
                     /locus_tag="ACG8KR_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0201578.1"
                     /translation="MKNLQLKFIVDPSIVRWFRIINQFERNQLATKGELAKSNHVSER
                     TIQTDVNKMKDYFSESVQFVSTKSGYSFQKERPVLFLKQKEQLLENEILFELLGNVFY
                     GELEDMTELIDRFHLSEATFRRYLKQIQPVLEEYGLELSLYPLDLKGEEANIRKFFKD
                     FYYEGEMTPHTLVPPRDLHELVQATFYDKFEYFSIGTGTSPAEFYYSLYIAIERVRQG
                     KTIAIPQGLIERVIASENFRLMYSLKEAIQTNYQVALSEEEFCWLY"
BASE COUNT         3260 a         1477 c         1884 g         3044 t
ORIGIN      
        1 ccaatcatat ggaagccacc gattaggtat tttatgggtt tagtcggaaa tgtttcaaga
       61 gcaatttcca tcatatcgat aatgccatga tgactacagc cagtaaagac aatcagaccg
      121 tcttcttgct caattacgac caataattca tctgaaaaat catcataaac aaatttatca
      181 tccatctttt tgtataaatt cttggtggtc gagtttgctt tgcctagggg gatgatatgg
      241 aagctcggag caagttctgt tttcgttttt attgtatgta aattctcatg cattttaagg
      301 atttcatttt taggaatacc aacatacaga aacgtggtta aaatctttaa ataatggggt
      361 aaagttacac cttcagctgt ataaataggg gcgtttcctg attttaaata tcttaacccg
      421 ccggtgtggt cagaatgacc gtgtgaaata atcactgcct ccagatcatt caaagtttcc
      481 cccattttag tcgcattata gactaatttt ttattagcgc cagcatcgaa gagatatttc
      541 ttccctgagt agttaattaa gagagataac ccatgtccac ttttaaagtt caaatttatt
      601 cttgagtttt caaataaaac cttgatttcc actataacat ctcctttcac atgaacgtgt
      661 attcatatat tgtagcacat taagttctat tttttgttgt aattaccgaa aaaaatatta
      721 atggacgctt tttacgcttt atttcgtatg tggaagaaaa tttcttagga tgtggcgggg
      781 gattggggca aagctaaatt ccaattacag tgatcattta attagacacc tttaaatacc
      841 cacactttca tcaacactat aatttaggga aagaaaagtg ttggcatgat atagaaaaac
      901 ctttaattaa cagcgtttcc taaaattggc acgcatcttg cttatagaaa agcgtaagga
      961 taggaggaat gaaaatggca actataattc ttgccagcca tggggaattt gctcaaggat
     1021 taaagcaaac agctacaatg attattggag atagtgtacc aattcatgcc ttgtcagcat
     1081 ttagagatga agatgaatct attttggtac aaataaaaaa acttttagcc acaatagata
     1141 tagaagaaac ctacatttta actgatattc taggtggtag tgtgaataat gatatgctca
     1201 ctattattaa agagtatgag ggattgcaat taattactgg aatgaactta cctttagtta
     1261 tttctattgc aacctataat ggaaaaattg gggaaagtga gctagaaaag cttattaaag
     1321 agagtcaaca atcagtgatt gattgtagaa aattattagt agaacaactt actggaggag
     1381 atgacctatg attaagttag tacgtattga ccatcgtttg ttgcacggac aggtagtatt
     1441 ttcttggagt aaatcattaa atatcaatcg tattattatt gccaataaag aggcagcgaa
     1501 tgatgaattc aaaaaaatgt ctttgaattt atctaaaccg actggcatta aattaacaat
     1561 tttttcagtt gaacagttac tagaaaaaat gcctaaaatc gaaacactta acgaaaatat
     1621 catgcttatt tttggtaata caaaagaaat tcgtcaattt tgtgaagcct atcctaaagt
     1681 acaagaaatc aactatggag ggataatcaa aaaagaaggt tccaagcaat ttagtaatgc
     1741 cattttcttg aatgaatctg agcaagaaga tgccaaaaaa ttaaaagctc ttggcattaa
     1801 acaatttatt caacaggtac caacttctaa aaaagaagat ttgaatacaa tgttataaag
     1861 gaggaatgaa aatgctcgtt cagtcaattg gtttagggtt aatcggtgtt ttttgtattt
     1921 tagactcaag gcttttagga agaatgaatt ttgaacgacc gttgattgtc agtacgcttg
     1981 ttggtttggt acttggagac atggaaaaag gattaattgt tggagcctct ttggaattga
     2041 tgtccttagg attagtaaat atcggagctg ctgcaccacc tgatatgaat atggcatcaa
     2101 ttattgctac agcctttgcc attttatcaa atgcgaatgc agaagcagca ttaacgattg
     2161 ccattccaat ttcagtgtta ggtcaaatac taggtatctt aatgcggacg attttgtcaa
     2221 atttgactca cgttgcggat cgctttatcg aacaagggaa atttagccaa gcaaggagaa
     2281 ttcatatact ttatggctcg ggattatatg ctctcatgta ttttattcct atttttctag
     2341 caatttattt tggaacgaat cttgtttctc agattgttga tgcgattcct gagtggctaa
     2401 caaatggctt aacggttgca agtaaaattt taccagcata tgggtttgcg ttgttattac
     2461 aaacgatgtt aacgaaaaaa atggtgccat ttttattact tggattttta attactgctt
     2521 attctggatt gggaatcatt ggtattgcag ccttcgcatg tattgttgcg tttgtcatgg
     2581 ttcaagttca aggtaaaaac tcaaatggta accaaaatga gccagatcca ttagatgact
     2641 tataaggagg tagattaagt gaaagagaaa gatcaaaatg catggaaata tcggcagttc
     2701 ttatggcgtt catgggcaat tcaagcctca tggaactatg aacgacaaat gaacatgggt
     2761 tttatgtacg gaattgcacc aattctagat aacatttaca agaatccaga agatgaagca
     2821 atgaagaaag aagcttacga gcggcatatg atgtactaca actgtacccc tcaaacaagt
     2881 gcatttgtgt tagggttaac tgcttctatg gaggagcaat actacgaaga ccaagaaaac
     2941 tttaatccgg aatcaatcag tgcagtgaag acttctctga tgggaccgct atctggtgtt
     3001 ggtgattctt tctttcaagg aacgattcgg gtattagcct ttggactagg aattaattta
     3061 gcccaacaag gtagtatttt aggtccaatt ctagcaatgt taatttcctt tgtaccttcc
     3121 tttttagtta cttactatgg tgggaaaatt ggctataaca tggggaataa atacttgtct
     3181 aaactgtatc aagaaggttt gatggataaa gtaatgtatg tttgctccat tgtggggttg
     3241 atggtcattg gttcgatgat tgccagcatg atcgggatta caacaccaat tcaaatgggt
     3301 aaagcttttg tcttacaaga tgtattagat agtattatgc cacaattttt accattagga
     3361 ttaaccttct tcatgtactg gttattgcag aaaaaggtca aaacaggatg gatgttggca
     3421 atctgtttag tcggaggaat tatttttagt ttgttaggta tcttacaata agggaataac
     3481 caataaattt ggttgaataa tataaataag gaggaattca attgatgaat tcaaaagaaa
     3541 ttgttaaaga aatcgtagcg aaaaaagagg taaaatcaat cgtatttgtt ggctgtggcg
     3601 cttcaaaagc ggacctttat cctgcaaagt attttctaga ccaaaatgcg aagcagttac
     3661 ggattagtca ttacacagcc agcgaattta actatgcaac accagcatct gtagatgagt
     3721 caactgtcgt tatttcagct tctttaggag gtacgactcc agaaacagtt gaagctaacg
     3781 cagtagctaa agaaaaaggt gctacagtta ttagtctaac acgaagcatt gactcaccgt
     3841 taacaaaaga tgcagattat gtaatttatc atcgatttgc agagaactat gctgctaaat
     3901 tagaaaaaac aggctactgt ttagaattgg cagtagaact tttacatcaa gtagaaggtt
     3961 atgaccatta tgaagcaatg caagaaggat ttgctactat ctatgatctt gcccaaaaaa
     4021 gtgcagaatc agctcgtaaa gatgcgaaga aatttgctga acgctttaaa gatgaaaaag
     4081 ttatttatgt aatgagtagt ggtgctacac atgaagtagc atactcaaca tcaatttgct
     4141 tgatgatgga aatgcaatgg attaattctg gtaccttcca ttcaggagaa tttttccacg
     4201 gaccattcga aattgtggat aaagacgtac cgtttatatt attaatgaat gatggaaaaa
     4261 ctagaaatat agatgctcga gcattaactt tccttgaacg ctttgatacg aaaacagaag
     4321 ttgtagatgc cttggattat ggcttgtctt cacatattgc taaagaagtc ttagactact
     4381 ttaacccatt cgtgattact gcagtattta gagtttatgc agaagaattg tcagaagcaa
     4441 gagaacatcc attaactaag agacgttata tgtggaaact aacttattaa aaaatagatc
     4501 aaactaaata aggtggcttg agaaattaag ccaccttgtt ttcttcaatg tgattttata
     4561 catatggtaa aataactagt acatattgtg aaagcaggtg gcaagatgca gcgattaaat
     4621 gactatcttt atcaactaac attgcttttg aaatctacgc cttcagataa ttattcagct
     4681 cgcagtattt cgcaagcttt acagttgaat agaagtacaa tcagtagcta cttaaatgaa
     4741 ggtgttcgtg aagggctttt cataaaagta aaaagttacc cggttttatt tttacataga
     4801 acagctcttg aagaactaca tatcacactg aataactccg aaatagaatc aattgaaagc
     4861 ttgttaacaa tttctcaaaa acccgcatta gatcaagtaa ttggctctaa aggaagtttg
     4921 aaagaagcaa ttgatcaaat taaaacggca gtcttatatc ctggaaaagg gttgccttta
     4981 ttattgatcg gcgctagtgg atcagggaaa acctttctag cgaataaaat atatgaatat
     5041 gcagtagaag aaaaggtcat tcacacatcg gcacctttca tagattataa ttgtgcacaa
     5101 tatgtgagca atccagagtt actatcctct gctttatttg gctacacgaa aggagcattt
     5161 accggagcaa gccaggagca tactggtttg ctagaaaaag ccgatggagg agtattgttt
     5221 ttagatgaag tgcatcgctt atctgaagaa gggcaagaga aattatttac atttatggat
     5281 acgggtgaat tctctcctat gggggataat agtattcgga aaaaagccga tgttcgatta
     5341 gtctttgcaa caacagaaaa tatatatacg actttcttac caacttttct taggagattg
     5401 ccagttattg ttaatctacc tagatttcaa caacgtccgt cgtttgaacg tctgtcattg
     5461 attgatgaat tttttgtttc tgagagtaaa attttagcta aggaactatc tgtttctgat
     5521 gcattgattc atttcttaat gaatagtgat tttgaaggta acgtggggaa tattaaaaat
     5581 attatcaagt atgcttgcgg aaacgcatat atccaccaga agagtcagca aacaattcaa
     5641 gttaaattat tggatatgcc tttagagtac agttataaat tcaaggagca actaaataaa
     5701 cccagaaaga aaagagcaga tcgaatctac tatccagaag aaacacgaca agtgcaacta
     5761 gaaagaaaca atcagctttt tgttcaattt tttgaccaat tacttactgg attctctgaa
     5821 gtgattgaaa ataacaagga tattaaacta tttttagaag aaatggttac caaggtaaca
     5881 caagtaatgg atacgcttat ttttcaagag gattacgaaa aagagcagtc tttatacgcc
     5941 attctaaact atcatattcg tcaaagtatt gattttatgc gagaaagcta cgggtttgaa
     6001 caagatggta accgggtagt cgctttggcc aactatctat atgtcaagga taatcaagtg
     6061 atttttgaaa atagtcttca agaaaagaaa accaaagatc agcttaacga attttttgct
     6121 aatgttcttg aaacccctta ttggtatgca aaaaaaatct taagcctttt atctgagtgt
     6181 ttagatcaac ctgtgtatga agaggacatt ttctttatta cgctatattt ttatagtttg
     6241 aatgtcacga atgttaaaac aggtattaag agtattgtgc tagctcatgg ttattctaca
     6301 gctagtagta tggcgaatgt tgccaaccgt atgctgaata agaacgtttt tcaagcttat
     6361 gatatgcctt tagatattac attagagaaa attgaagcaa aattgattca attccttgga
     6421 gattatcgaa cagattcagg cttgatttta ttggtagata tgggttcact aaaccaacta
     6481 ggcggtttat tagtgaatca tctaaatgga ccactgttaa tcattgatca tatcaataca
     6541 ccgttattat tggaagtcgg ccaacaagta ttaaacaata aaagtatcac cgaaatagga
     6601 gcagaaattg aaacaagtct tactttcaaa aagcagttac tacttcccaa gaaaaagaaa
     6661 aagaaagcca tcattacttg ttgttataca gggattggta gtgcgaccca gattcaggag
     6721 attttaacga aatgcttgaa agatgcagtt agagaattag taattattcc gtatgattac
     6781 aaaaagctgt tagaaaacaa gaattttgag actccttttc aattatacga tgtcatcatg
     6841 attgtaggta cagaagatcc caaaattaat cagattccat atattggatt agatcgcctg
     6901 attaatgggg aagcaattgg cgaatttgtt cattacttgg aaaaagagct gactattgaa
     6961 gtgaacgatt tacaaaaaga attaattttc aatttttcaa ttagtaaaat tgtcgaaaat
     7021 ttaaccattt tggatccaga taaggtgcta tcttttgtac aaaaagcaat taaagaatta
     7081 tcgaagttat taaattttga tttttctaat aatcagctgt ttttgttgta ccttcactgt
     7141 agttgtatga ttgaacgaat tgtacgtaaa gaaagtgtgg atgagcaaga ggacattgaa
     7201 gaatatatgc aagcaaatgg agaaataatg gataaaattc atagcgcttt ttttgagatt
     7261 gaaagggaat acacaattga aataccagat ttagagttgc gattattgag cgatattgtt
     7321 caaggatagg agtgaattcg aatgacaatg ggagtaattt ttgatatgga tggggtactc
     7381 atagacagcg agacatttta ttttgagaga agaatgaact tctttcaaga actggagata
     7441 gatccaggat caaaccagat tgatgattat gtggggaaaa cagaagctgc aatatgggaa
     7501 acattagttc ctgaggacaa atcattaaga gaggaacttt atccgaaata tgtagattat
     7561 cgaaaaacgc atccaattga tttcgctaaa gatttacgaa aagaagtgaa accgttactt
     7621 gacttcctca agaagcggga tataccattg gcactggcat cttcttctcc agttgttgag
     7681 atagagagta tgctcattca atgtgaactt actgaatatt tttcctttgt tataagtggt
     7741 gagtcattaa aacaaagcaa accacatccc gaaatatatt taaaagcgaa acagcaatta
     7801 gactgtgact tctttattgc agtagaggat tcaacgattg gtattaaatc agcaaaggca
     7861 gcaaatatct atacggtggc actaaaacaa ccaatgcaaa tcgatcagag tgaagcagac
     7921 atacaaattg aaagtctaca agaactggaa caaattgttt tagaaaaagg ttagtgaatt
     7981 aatggaaata aggctatata tctgtgctag ctattttgac taaccaaaaa aacttgctat
     8041 aattcaggtg tagtaaaagg tgctggtaac accttttacg aaaatgcttc ggcagtgata
     8101 atgggattgg ctttttaagt tttttatttt gttcgcaatc cctaaggtca tgtgaatgat
     8161 cctagggatt gctagaatat tttgcaacat tctatctatt tcagaacgta accttcccca
     8221 gttcattggg attcattagc atacaaccaa catttcaaaa agaaattgca ctacaccatt
     8281 attagctatt ttgactaacc ccaaaaaact tgctataatt caggtgtagg taaaaggtgc
     8341 tggtatcacc ttttacgaaa ctgcttcggc agtggtaatg agtgtggctt aaaggactag
     8401 tcctttaagc tttttacttt gttcgcaatc tctaagatca agttaatgat cgataagatt
     8461 gctagaatat tttgcaacat tctatttcag aacgtaacct ttcccattcc actgtgattc
     8521 attggcaacc ctcctcacaa ccaacgtttc aaaagaaatt gtaaagttgt tggggtacca
     8581 cgaccgaaca atactacacc tgaatgatag taagttgtct cattaagtat agcatatcct
     8641 ctttttgggt aaatagaaat cggttacatt ttttatccaa gaccaagttt tttcatttgg
     8701 tcttatttgc tatacccaaa caattccgca gtatagtcga cggtattgat tttatcaggt
     8761 gagtaataga aacttgtttt ccttccccaa aacattacct ataaaggaga aaaaatggta
     8821 tcgtaattat ataggtatag gatgaaattt ttttctaaga ggagggttgt tgatgaaaaa
     8881 tttacagtta aaatttattg ttgatccatc gattgtacgc tggtttcgga ttatcaatca
     8941 atttgagcgc aatcaacttg ccacaaaagg agaattagca aagagtaatc atgtatctga
     9001 acgaaccatt caaacagatg tcaataaaat gaaggactac ttttctgaaa gtgttcaatt
     9061 tgtttcaacg aaaagtgggt attcttttca gaaggaaaga ccggttcttt ttctcaagca
     9121 gaaggagcaa ctactggaga acgaaatttt atttgagttg ttagggaatg tcttttatgg
     9181 cgaattggaa gatatgacgg agctaattga tcgttttcat ttatctgaag cgacttttcg
     9241 tcggtatttg aagcagattc aaccagtttt ggaagaatac ggcttagaat tgagtcttta
     9301 tcctcttgat ttaaaaggag aagaggcaaa tattcgcaag ttttttaaag acttctatta
     9361 tgaaggggag atgacgcccc atactcttgt tccaccaagg gatcttcatg aacttgtgca
     9421 agctacattc tatgataaat ttgagtattt ctcgattgga acagggactt ctcctgctga
     9481 attttattat agtttgtata ttgcgattga gcgtgtgaga caaggaaaaa cgatagccat
     9541 tcctcaaggt ctgattgagc gagtaattgc tagtgagaat tttcgcttaa tgtattcctt
     9601 gaaagaagcg attcaaacaa attatcaagt tgctttatca gaagaggagt tttgttggtt
     9661 gtatt
//
LOCUS       DAZKLD010000112         9630 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.112, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000112 DAZKLD010000000
VERSION     DAZKLD010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9630)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9630)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9630
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.112"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..195)
                     /locus_tag="ACG8KR_002250"
     CDS             complement(<1..195)
                     /locus_tag="ACG8KR_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0201579.1"
                     /translation="MLKISYKPSTDSKEMKKEYETVNDFLQGQYLEVPPLQDHFVVTT
                     VTLDGKEIEMPDQTISGLFNY"
     gene            complement(211..825)
                     /locus_tag="ACG8KR_002251"
     CDS             complement(211..825)
                     /locus_tag="ACG8KR_002251"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HFQ0201580.1"
                     /translation="MFERSPSELTERENYKLLIGSVIPRPVAVVTTKSAKDIVNIAPF
                     SYFNIVSSNRPILSLAIQRKQDAVKDTAKNILETREAVIHILDEDNVEEVNKTAANLP
                     NEESELYLTDLTLTDSVIISVPALTEAKVRFETSLYEHVEIRNKNKVTADLFLLEVQK
                     YHLNETVYDDKTGRIDPRKLNAVSRLAGNDYAAIGEIFTIARPE"
     gene            complement(972..1655)
                     /locus_tag="ACG8KR_002252"
     CDS             complement(972..1655)
                     /locus_tag="ACG8KR_002252"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009385 - N-acylmannosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HFQ0201581.1"
                     /translation="MDTLEQIKGGVVVSCQALENEPLHSSFIMGRMALAAKEGGAVGI
                     RANTTEDINEIKRIVDLPVIGIIKRDYDGSEVFITATEKEVEELLETQAEIIALDATS
                     RKRPTEKTTAELVQMIHEHGRLAMADISTFEEALQAQEDGFDLLSTTLAGYTEYSRKT
                     QTPDFDLLNQIIEQVDIPVIMEGHTDSPEQVEKAYELGAFSVVVGSIITRPQLITKRY
                     VEAASKANN"
     gene            complement(1764..2222)
                     /locus_tag="ACG8KR_002253"
     CDS             complement(1764..2222)
                     /locus_tag="ACG8KR_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HFQ0201582.1"
                     /translation="MYQQVEEYVKKFQEKYPSYQSEKLGFFSFGDTEKMADELSELVV
                     KKIKTGTSSGYDLYKQENEPLPKVGQLDVVLNGKNEPVCIIKNINVTILPFSEVTKEH
                     AWKEGEGDRTLAYWRKVHAEFFKHAYKEALNIEFTEEKLVVYEEFEVIFF"
     gene            complement(2306..4069)
                     /locus_tag="ACG8KR_002254"
     CDS             complement(2306..4069)
                     /locus_tag="ACG8KR_002254"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HFQ0201583.1"
                     /translation="MSTVLHVLDAINSPADVKKLSVEEMKQLAEEMRQKILQKDSDVG
                     GHVGPNLGVVELTIAFHYVFDSPKDKIVWDVSHQSYGHKLLTGRKNGFEKGHYREVSG
                     YSNQHESKHDYFTVGHTSTSISLATGLAKARDLKKESGNVVAFIGDGSLSGGLAFEGL
                     NNGGVLQSNLIVLVNDNEMSIDENHGSLYKNLAELRQTNGKSSNNLFKAMGFDYRYVD
                     NGNDLETVIRLFEEVKDIDHPIVLHVHTEKGRGYQPAIDNKMKYHWHVPFDLSTGEAK
                     QVTSGETFNMIIMDYLDKKMQTSTAPIAAVNAAIPGAFGLKTFQEKHPDHYFDVGIAE
                     QHSITFASALASQGVRPVVFQSSTFLQRAYDQLSHDLAINENPVIILVGGGTISASDR
                     THQGTFDIPYLSSIPNIKYLAPATKEDMISMLDWAFEQTAQPVAIRIPTHGVEHGTAI
                     ANDYSIPAYQTVNNGGKVAILALGGFLELGKETVKAFRKKGIEATLINPGTITENDVE
                     TLDKLKETHELVITLEDGSLSGGFGENISRYYGDSSMNVLNFGAEKEFTDSVPVGQLY
                     ERYHLTPKQIVEDSLRVLNIQ"
     gene            complement(4290..5750)
                     /locus_tag="ACG8KR_002255"
     CDS             complement(4290..5750)
                     /locus_tag="ACG8KR_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0201584.1"
                     /translation="MLENYIERNIFRKVYLCEQLFEFQEIDIEQTAISLRVTTPTILH
                     DLESLAECLEYCIKEQVREKHKYKLVFKHGIALSELTQFLYGQSYFLKFLSYYFNGIF
                     TSTELADLEFISLSKVYTIKKIVLDFFKENDYLKNKEIIIPEFDSRNILLALVRYVNW
                     EGYENKNPYVEEACNELIDYVETHFFKRRYSEDEKSFIIRGIEIAIGRKAFPLGLKEE
                     DKKSAETKPLFQLVCQGLEEQKGILDLQEEDVYYIFSLFNSRNYTNENMELLRKDFEV
                     VYKNFVLNDLSLNELILQIISIAEIDDAENFIFKQSFLQFVRTLWADGQVFLPERIYL
                     LSEKEQLLYGKIVEILEEWKKKNQIQLRWNENFIRKFVKSLSLINSEQAGRQEIEIFV
                     VSESSVKQFFYRTQFHLYAEEGIAEINPILFRSLEELPDECLCAKKRVVLCDAASYQN
                     GLETEKTKIYPISLKGANIHLYHIAQELHLLKKHIS"
     gene            complement(5958..6884)
                     /gene="glsA"
                     /locus_tag="ACG8KR_002256"
     CDS             complement(5958..6884)
                     /gene="glsA"
                     /locus_tag="ACG8KR_002256"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HFQ0201585.1"
                     /translation="MEVKELEQLIGKNFHYYQTGQVADYIPALAEVDPKQLGMAIYDL
                     DTKQLIEGGDADVRFAIESMSKVPVLLLAIKDNGIEKVFQTINTEPTGFAFNSIMNME
                     INHRKHPMNPFVNAGAIATTSLIAGKDADEKFERILSFMKEICDDPNITLNEEIYHSE
                     SRTGDINRSLAYYMKGNQMIEGDVPEILDAYFRQCSLNVTATGIAKLAAVLANKGIAP
                     WNGKQIITEESATIVKSIMTTAGLYDESGEFSVHVGVPAKSGVGGGLMAAVPNRYGIG
                     VFSPALDPFGNSAAGIQLLKDVVKELDADIFE"
     gene            complement(6907..8334)
                     /locus_tag="ACG8KR_002257"
     CDS             complement(6907..8334)
                     /locus_tag="ACG8KR_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFQ0201586.1"
                     /translation="MQQSKTKIPAKKLTFFGFLAMTISMVVSLYEYPTFATSGFSLVF
                     FLLLGGLLWFIPVALCAAEMATVKGWEKGGVYTWVSRTLGKRFGFAAIFFQWFEITVG
                     YLTMLYFLTGALSYATGIGAIQNNKYLKLAILLIIFWAILLSQLKGTKYTSLIARIGF
                     VAGILLPALVLFGLGIHYVASGAPLQTILSMKTLIPDFSKLPTLVVFVSFILAYMGVE
                     TSASHANEMENPKKDYPLAMFVLVILAIILDTFGGLTVATTVPQHGLSLNTGVIQSLE
                     FLLRHIDPNLAWIAKILAILVCLGVIGEIASWVTSPSKALHVAAEEGLLPEYFAKENT
                     HGVPAHLMIANGIVATIWAAVFTLSGGGNNLSFLAAMSLTVVIYLMLYFMFFAGYLKL
                     VFKDDSLERAYQVPGGKVVKVIVACLGLLTSLFAFCISFVPPASIAAGQHGIYETILI
                     VSYLIVLVIPFTIYACRHKFQHAEK"
     gene            complement(8365..9579)
                     /locus_tag="ACG8KR_002258"
     CDS             complement(8365..9579)
                     /locus_tag="ACG8KR_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium channel activity
                     [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HFQ0201587.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
BASE COUNT         2851 a         2019 c         1606 g         3154 t
ORIGIN      
        1 gtaattaaac aatccgctaa tcgtttgatc tggcatttct atttccttac catctaaagt
       61 aacagtagta acgacaaaat ggtcttgcaa tggcggaact tctaagtact gcccttgcag
      121 aaaatcattc actgtttcat attctttttt catttctttt gaatctgtcg atggtttata
      181 gcttattttt aacataacta gtgctccttt ttattcggga cgtgcaatcg tgaaaatttc
      241 tcctattgct gcataatcat ttcctgccaa acggcttaca gcattcaatt ttcttggatc
      301 gatccttcct gtcttatcat catagactgt ttcatttaaa tgatatttct gtacttctag
      361 caaaaataaa tcagctgtta ctttattttt attccgtatc tcgacatgtt catataagga
      421 tgtttcaaaa cgaacttttg cttctgtcaa tgctggtact gaaatgatta ctgaatcagt
      481 taaagtcaag tcagtcaggt acagttcgct ttcctcatta ggcagattgg ctgctgtttt
      541 atttacttct tctacgttgt cctcatcaag gatatggata acagcttctc ttgtctcaag
      601 aatattcttt gctgtgtctt ttactgcatc ttgttttctc tggatcgcca aggataagat
      661 gggtcgattc gaactgacga tattgaagta actgaagggt gcgatattca ctatatcttt
      721 tgctgattta gtcgttacta ctgctactgg tctagggatg actgaaccaa tcagcaactt
      781 gtaattttca cgttctgtca attctgaagg actccgttca aacatggttt cgccttcttt
      841 ctataaaatt cgttaaaaag cagatcttga agacaaaatg aaaagtaatg cttttttcat
      901 cttcttagaa tctgctttaa actatctatc aatttttctt ttttgtcaag aatctgtgcc
      961 gtgcgtgttt tttagttatt tgctttactt gcggcttcga catatctttt agtgatcaat
     1021 tgcggacggg tgataataga tccaactacg actgaaaaag cacctaattc atatgctttc
     1081 tctacttgtt caggagagtc agtatgtcct tccataatta ctgggatatc gacttgttct
     1141 atgatttgat tcaataaatc aaaatcaggt gtctgcgtct ttcgtgaata ttctgtatag
     1201 cctgctaaag tcgtagacaa aagatcaaaa ccatcttctt gcgcttgaag cgcttcctca
     1261 aaagtcgaaa tatctgccat agctaaccga ccgtgttcgt gaatcatttg gacaagttca
     1321 gcagttgttt tttctgtcgg acgtttacga gaagttgcat ccagtgcaat gatttctgct
     1381 tgcgtctcca aaagttcttc cacttctttt tctgtcgcag taataaacac ttctgaccca
     1441 tcgtaatctc ttttgataat ccctattacc ggtaaatcaa cgattcgctt gatttcattg
     1501 atatcttctg tcgtatttgc tcgaatacca actgctccgc cttcttttgc agccaaagcc
     1561 attctcccca taataaaaga actgtgtagc ggctcgtttt ccaaagcttg acaagaaacg
     1621 acgactccgc ctttgatttg ttccagtgta tccacaatat ctcctccagt aatgttatat
     1681 aaaaatacag cttcatataa aaatgtaacc cttttctatt caggagacaa taatgagcta
     1741 tttctttcag cagacctttc ttactaaaag aaaattactt caaattcttc gtatacgacc
     1801 agcttctctt ctgtaaattc tatgttcaac gcttctttat aagcatgttt aaaaaactca
     1861 gcatggactt tccgccaata agctaatgtc cgatcccctt ctccttcttt ccaagcatgc
     1921 tctttcgtga cttcactaaa aggcaagatg gtcacattta tatttttgat gatacaaact
     1981 ggttcatttt ttccattcag gaccacgtct aattgaccaa cttttggtaa aggctcattc
     2041 tcttgtttat acagatcgta accacttgat gtaccagtct ttatcttttt tacaactagt
     2101 tcgctcaact cgtctgccat tttttctgta tccccaaatg aaaagaaacc taatttttct
     2161 gattgataac taggatattt ttcttggaac tttttgacat attcttctac ttgctgatac
     2221 ataacatcca cccctttgct ttttataatg aaaaaaccat ccagatacta gtataaacaa
     2281 ttatctggat ggtgagcata atgaattatt gaatattcaa gacacgcaga ctatcttcaa
     2341 cgatttgttt tggtgttaaa tgataacgtt cataaagctg ccctacagga acgctatccg
     2401 tgaattcttt ctctgcacca aaatttaata cgttcattga agaatcacca taataacgac
     2461 tgatattttc gccaaatcct cctgacaaac taccatcttc taatgtgatg actaattcgt
     2521 gtgtttcttt taatttatct aatgtttcta cgtcattttc tgtgatcgtt cctggattga
     2581 tcaacgttgc ttcgattcct ttttttctaa aagcttttac tgtttctttt cctaattcaa
     2641 gaaaaccgcc taaagctaaa attgctactt ttcctccatt atttactgtt tgataggctg
     2701 gaatactata atcatttgcg atagctgtac catgttctac accatgtgta ggaattcgga
     2761 ttgctacagg ctgcgctgtt tgttcgaacg cccaatctag catagagatc atatcctctt
     2821 ttgttgctgg agcaagatat ttgatgttcg ggatacttga taagtaagga atgtcaaatg
     2881 ttccttggtg tgttcggtca ctagctgaga tcgttccgcc accgactaaa ataatcactg
     2941 gattctcgtt gatcgctaaa tcatgtgata attgatcata tgcccgttgc aagaaagtac
     3001 ttgattggaa gacgactgga cgtactccct gagaagccaa agcagaagcg aaagtgatag
     3061 agtgctgttc agcgatcccc acgtcaaaat aatgatctgg atgtttttct tggaatgttt
     3121 tcagtccaaa ggcgcctggt atcgctgcat tgactgcggc aataggagct gtacttgttt
     3181 gcattttctt atccaaataa tccatgatga tcatattaaa tgtttctcca gaagttactt
     3241 gctttgcctc cccggtggac agatcaaatg gtacatgcca atgatatttc attttattat
     3301 cgattgccgg ctgataaccg cgaccttttt ccgtatggac gtgaagtaca atcggatgat
     3361 caatatcttt tacttcttca aataaacgga tcaccgtttc cagatcgttt ccattatcca
     3421 cgtaacgata atcaaatccc attgctttaa ataggttatt gctgctttta ccgttggttt
     3481 gacgaagctc agctaagttt ttatataaac tgccatgatt ctcatcaata gacatctcat
     3541 tatcgtttac taatacgatt agattggatt gtaaaacacc accattgttc aatccttcaa
     3601 aagctaagcc accacttagg gagccgtcac cgataaatgc taccacattt cctgattctt
     3661 tcttcagatc tctcgcttta gctaaacctg ttgctaagct gatagatgta gatgtgtgcc
     3721 ccactgtaaa atagtcatgt ttactttcat gttggttgga atatccgctc acttcacggt
     3781 aatgtccttt ttcaaaacca ttttttcgac cagttaaaag cttatgacca taagactgat
     3841 gggaaacatc ccaaacgatt ttatcctttg gtgaatcaaa tacataatga aacgcaatcg
     3901 ttaattctac tacacctaga ttcggaccaa cgtgcccacc tacatcgctg tccttttgca
     3961 agattttttg gcgcatctct tctgccaact gtttcatttc ctcaacagat aattttttga
     4021 catctgcggg gctattgatt gcatccaata catgtaatac tgtactcata atacattctc
     4081 tcctccgtac tatcttcttt taatgaaaat agatttgtta cactttatct attctactac
     4141 ttagagttga ctctaagtca atagcatttt aaaaaaattt cagagaaagc ttgttatata
     4201 agtagatctt cgcacgaatc aggactcaaa taaaaacctt ctacagacaa ttaaaaagct
     4261 ttgtagaagg tctcctaatt aaggtctcat caagaaatat gtttttttaa taggtgaagt
     4321 tcctgagcaa tatgatacaa atggatgttt gcacctttta atgagattgg atagatcttt
     4381 gtcttttcag tttctaatcc attttgatag ctagcagcat cgcacaaaac cactcttttt
     4441 ttcgcacata gacattcatc aggcaattct tctaatgaac gaaataaaat aggatttatt
     4501 tcagcgatgc cttcttccgc atacaaatga aattgggttc gataaaaaaa ttgtttgact
     4561 gaactttctg aaacaacaaa aatttctatt tcctgtctcc ccgcttgttc actgttgatc
     4621 aaagaaagac ttttgacgaa ttttcgaatg aaattttcat tccatcttag ttggatttga
     4681 tttttctttt tccactcttc caatatttct acaatttttc cgtacagaag ttgttctttt
     4741 tctgatagaa gatagatcct ttccggtaaa aatacttgtc catctgccca caacgtacgg
     4801 acaaattgta aaaaggattg cttgaaaata aaattttctg catcgtcgat ttcagcgata
     4861 ctaataatct gtaaaatcaa ttcattcagt gataggtcat tgagaacaaa atttttataa
     4921 acaacttcga aatcttttct taacagctcc atgttctcat tggtataatt tctactgttg
     4981 aacaaagaaa aaatataata gacatcttct tcttgtaagt ctagaattcc tttttgctct
     5041 tccaatcctt gacagactaa ttgaaataac ggcttcgttt ctgcgctttt cttatcttct
     5101 tctttcagtc caaggggaaa tgcctttctt ccgattgcga tctctattcc tcgtatgata
     5161 aaggactttt catcttccga atatcttctt ttaaaaaaat gagtctctac ataatcaatc
     5221 aactcgttac aagcttcttc tacatacgga tttttatttt catagccttc ccaattgaca
     5281 taacgtacta aagctagcaa gatgttccgc gagtcaaatt ctgggataat gatttcttta
     5341 tttttcaaat aatcattttc tttaaaaaaa tccaatacga tttttttgat tgtatatact
     5401 ttgctcaaag aaataaattc aagatcagcc agttctgtac tcgtgaatat accattaaaa
     5461 taataactta aaaactttaa aaaataagat tgaccgtaca gaaactgtgt gagctcagaa
     5521 agagcaatac catgtttgaa tacgagttta tacttatgct tctctcttac ttgttctttt
     5581 atacaatatt ctagacactc ggctagactt tctagatcgt gaaggatcgt tggtgtagtc
     5641 acccttagag aaatagctgt ttgttcgatg tctatttctt ggaattcaaa taattgttca
     5701 cacagataca cttttctaaa tatatttcgc tcgatgtaat tttccagcat tcctgataca
     5761 cgcctccagt ttttcttatg attttattta atcatatctg taaatcgtat ttttgtttcc
     5821 tttttattta gaaaagccct ttcaaaaacc aataaaaaaa gaaactggga caaaagtcaa
     5881 acaagctcac aatttcagcc tcattgtagg tagctttgag ccgtttggac ttttacccca
     5941 gcctctttca agaatggtta ttcaaagatg tcagcgtcta gttctttgac tacatctttc
     6001 aacaattgga taccagctgc gctgttacca aatggatcta gtgccgggct gaagacacct
     6061 atgccgtaac gatttggcac agctgccatc aacccgccgc ctacaccgct ttttgcaggt
     6121 acaccaacat ggacggaaaa ctctcctgat tcatcataca aaccagcagt tgtcataatt
     6181 gatttgacga ttgtagcact ttcttcagta atgatttgtt tgccattcca aggagcaatt
     6241 cctttatttg ccagcacagc agctaatttt gctatgcctg tagcggtgac attgagggag
     6301 cattgtctga aataagcatc gagtatctcc ggtacgtctc cttcgatcat ttgattacct
     6361 ttcatatagt aagcaagaga gcgattgata tcacctgttc ttgattcaga atgatagatt
     6421 tcttcattca atgtgatatt aggatcatca cagatctctt tcataaatga caagattcgc
     6481 tcaaattttt catctgcatc tttaccggca atcaacgagg ttgttgcaat cgctcctgca
     6541 ttaacaaaag gattcattgg atgtttgcga tgattgattt ccatattcat gatagaattg
     6601 aaggcaaaac cggttggttc cgtgttgatt gtttgaaaga ctttttctat tccgttgtct
     6661 ttgattgcta ataacaatac gggaactttt gacatgcttt cgatggcaaa gcgcacatcg
     6721 gcgtctcctc cctcaatcag ttgttttgta tccaaatcat aaatcgccat acctaattgt
     6781 ttgggatcca cttcagctaa tgctggaatg taatccgcca cttgtcctgt ttgataatag
     6841 tggaaatttt ttccaatcaa ttgttctaat tcttttactt ccatttgaat aaccagcttt
     6901 cattttttat ttttctgcat gctggaattt atgacggcat gcgtagatcg taaatggaat
     6961 aactaagacg ataagataag aaacaatcag aatcgtttca taaatcccat gctgtccagc
     7021 tgcgatacta gcaggtggaa caaatgagat acaaaaagca aaaagtgaag tcaaaagacc
     7081 taaacaagca acgattactt ttacaacttt tccgcctgga acctgatagg ctctttcaag
     7141 actgtcatct ttaaaaacca gcttcaaata gcccgcgaaa aacataaagt acaacatcag
     7201 gtagattaca acagtcaatg acatcgctgc aagaaatgaa agattattcc ccccgccgct
     7261 taatgtaaag acggctgccc agattgttgc aacgatccca tttgcgatca tcaaatgagc
     7321 aggaacaccg tgtgtgtttt cttttgcaaa atattcagga agtaatcctt cttctgctgc
     7381 tacgtgaagt gcttttgaag gagaggttac ccaagaagca atttctccga tgacacctaa
     7441 acaaacaaga atcgcaagaa ttttcgcaat ccatgcaaga tttggatcaa tgtgtcttaa
     7501 taggaattct aacgattgaa tcacccctgt attaagagac aaaccatgtt gaggtactgt
     7561 agtagcgacg gtcaatccgc caaaagtatc taaaatgatt gccagaataa ccaatacaaa
     7621 cattgctaat ggataatcct ttttagggtt ttccatttca tttgcatgac tggcagatgt
     7681 ttctacgccc atatatgcta aaataaagga aacaaaaaca accagcgtag gcaatttcga
     7741 aaaatcagga atcaatgttt tcatagacag tatcgtttgt agtggagcgc ctgaagcgac
     7801 ataatggatc cctaagccaa acaagactaa tgctggcaac aagataccag ctacaaaacc
     7861 gatccgagca atcaatgaag tgtatttggt tccttttaac tgtgagagta aaatagccca
     7921 gaaaataatc aataggattg ccaatttcag atatttattg ttttgtatag caccaatacc
     7981 cgttgcatag gataatgcac cagttaagaa atacagcatg gtcaagtagc caactgttat
     8041 ctcaaaccat tggaaaaaga ttgcggcaaa accgaatcgt tttcccaatg tgcgactgac
     8101 ccaagtatag acaccgcctt tttcccatcc tttcaccgtt gccatctctg ctgcgcacaa
     8161 tgccacaggg ataaaccata gcaaaccgcc taataacaag aaaaatacta agctgaagcc
     8221 tgaggtcgcg aatgtgggat attcatataa tgaaacgacc attgagatgg tcatagccaa
     8281 aaagccaaag aacgttaatt tttttgcagg aattttcgtt ttgctttgtt gcatgaatct
     8341 tcctcttttc tatttgaatt tgtttcaatt gagtttgttg ccaaagaaga aaaagtccaa
     8401 tccttcttct tgttcttctt ttgtagtagt gatcgttgta aagtgcatcg ccgattttaa
     8461 aatcactgtt gtcagcacat aggcatacac ggcagtaatg atgacaccaa tcacttgaat
     8521 ccctaagaag taccagctgc ttggattcgt tagtaaatgg ctgttagatg caaagatccc
     8581 tatcagaatc gtacctgtga atccgcccat gccatgaaca ccccacacat caagtgcatc
     8641 atcccattgt ttctttttac gaaaatcaac agcagcatga cagacgatac ccgcaatgat
     8701 cccaatcagc atggcacttt ttgcttctac ataaccggca caaggcgtaa tggctgccaa
     8761 tccagcaacg gatcctgtta gtacgtcgac aaaatcaaag ccatttcctt ttacttttgc
     8821 aatgatcagc caagtaatca tcgcaaatgc aagagcgatg aatgttgaac caaatgcatt
     8881 gactgcttgt tcatctgctc gaagtgcacc accggaatta aaaccgaacc agccaaacca
     8941 aagcagtcct gtaccgatag cagctgccat aagactgcta tgctgacttc tttctcctaa
     9001 aggaatcttt ctttgaccaa gtgtgaggat acatgctaaa gagccgaatc cagctgtcgt
     9061 gtggatgact gctccaccag cgaaatctct aaatcccatc tgatccaaaa atccgccacc
     9121 ccatatccag tggcaaacag ggaaataaat cagcagattc caaaaaataa caagtataat
     9181 atagcctttt aaattcaatc tccctgcaaa agctcctgtc atcaaaggaa cggtaatcac
     9241 gataaacatc agttggtaaa gaaagaacat taaaaatgga atcgttgcac catgaaacat
     9301 gttggggaag cttgtaacat gtctcatcaa aaagaaatct gccgggtttc caataacacc
     9361 gccgatatcc cgtccaaagg ccaatccgaa tccaccaaat acccacataa tcgttgttat
     9421 cccaacggag agaaaagatt gagacatgat cgttagtata ttattcttcc gtgctaatcc
     9481 gccgtaaaaa aaagctagac caggagtcat caggcaaacc attgctgtac ataaaatcat
     9541 gaatgctgta tctcctgtat tgataggtaa tgtattcatt tttttcgcct cctttctacg
     9601 attacgagta taaaagaagg tgtagttgtt
//
LOCUS       DAZKLD010000113         9493 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.113, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000113 DAZKLD010000000
VERSION     DAZKLD010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9493
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.113"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            11..538
                     /locus_tag="ACG8KR_002259"
     CDS             11..538
                     /locus_tag="ACG8KR_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HFQ0201588.1"
                     /translation="MRKRGFEVISKYKDQNINLPQRATHHAAGYDFEAAEEVVIPSIW
                     KAGWKKGLETVADGLKKDVSENEKQLKPTLVPTGIKAYMGEDEFLQLANRSSNPLKRF
                     LVLTNGVGVIDSDYYNNEANEGHIMFQFTNFGFKDVVIKKGERIGQGIFLPFLKADQD
                     QTTAQRQGGFGSSSK"
     gene            601..1971
                     /gene="radA"
                     /locus_tag="ACG8KR_002260"
     CDS             601..1971
                     /gene="radA"
                     /locus_tag="ACG8KR_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HFQ0201589.1"
                     /translation="MAKKSKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTD
                     RRTRTSLTGEKAKPTKIADVVPKKEPRIKTKLEELNRVLGGGVVPGSMVLIGGDPGIG
                     KSTLLLQVSQQLAAIGGKVLYVSGEESAEQIKLRAERLGSINTEFYLYAETDMNEISR
                     AIEHISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKIAKTNGIAIFIVGHVT
                     KEGSIAGPRMLEHMVDTVLYFEGEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEV
                     ANPSQIFLEERLDGATGSAIVVAMEGTRPILVEIQALVTPTMFGNAKRTTTGLDFNRV
                     SLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECF
                     IGEIGLTGEIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGNWEAPKGIEIVGVATLAET
                     LKRVFR"
     gene            2005..3162
                     /locus_tag="ACG8KR_002261"
     CDS             2005..3162
                     /locus_tag="ACG8KR_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HFQ0201590.1"
                     /translation="MQKRVITLLMIIVGASLGISFLPSAWQAIGQQSNGWLNNTFTNS
                     LLGALIFFLLSLLLAKHISAIIKQVEQKLSEVSLTYLLFGAIGAIIGLTLGVVISTPL
                     YNMEIPFVNSVLPILLMIILGYLGLRMGTTRIEEWKKVFGSRSKKIEQETASQVESQL
                     LERKSGENFHKYKILDTSVIIDGRIYDITKTGFLEGTLMIPNFVLYELQYIADSGDSL
                     KRVRGRRGLDILNALQKEEGISVEMYDGDFEDISEVDSKLIKLAKLLDGVIVTNDYNL
                     NKVSEFQNVPVLNINALANAVKPVVIPGETMNVLVVKAGTERQQGVAYLDDGTMVVVE
                     DGQHYMNERIEVVVTSALQTAAGRMIFAKPAHAGRGIDNKKETEKKTKQSK"
     gene            3332..4792
                     /gene="gltX"
                     /locus_tag="ACG8KR_002262"
     CDS             3332..4792
                     /gene="gltX"
                     /locus_tag="ACG8KR_002262"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HFQ0201591.1"
                     /translation="MTKVRVRYAPSPTGHLHIGNARTALFNYLFARHNDGDFIIRIED
                     TDQKRNIEDGEKSQLENLAWLGMNWDESPENPGEYGPYRQSERKEIYQPLIDQLLASN
                     RAYKCYCTEEELEAEREAQRARGEMPHYAGTCANLTPEEQAEKEAQGLESVVRFRVPR
                     NTEYAFTDMVKGAISFESDNIGGDFVIQKRDGMPTYNFAVAVDDHLMKITHVLRGDDH
                     IANTPKQLMIYEAFGWTPPTFGHMTLIINSETGKKLSKRDESILQFIEQYRELGYLPD
                     AMFNFIALLGWSPVGEDEIFEHQELIKMFDPKRLSKSPAAFDAKKLQWVNNHYIKAMD
                     LDAFTDMCLPYLIADGRVAENPDEKTIEWVKKIVSLYQPQMSYAAEIVDLSTLFFNEH
                     PVLNEAAKEVLAGETVPTVLHAFKAQLEQMEVVDAPTVKAAIKAVQKETGVKGKNLFM
                     PIRVAVSGQMHGPELPETIELLGREKSLAHLNQVLN"
     misc_binding    4789..5028
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            5114..5650
                     /gene="epsC"
                     /locus_tag="ACG8KR_002263"
     CDS             5114..5650
                     /gene="epsC"
                     /locus_tag="ACG8KR_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase EpsC"
                     /protein_id="HFQ0201592.1"
                     /translation="MSWFTRAIDAAKRNDPAARSTAEIVLTYPGFHALFWHRFSHFLY
                     QHRLFLLAKINAQFWRFLTGVEIHPGATIGTGVFIDHGMGIVIGETAEIEDDVILFHG
                     VTLGGTGKETGKRHPTVKQGAMLSANAQILGPVTIGKNAKIGAGAVVLKDVPDDATAV
                     GVPAKVVRIKGEKVRRSE"
     gene            5647..7059
                     /gene="cysS"
                     /locus_tag="ACG8KR_002264"
     CDS             5647..7059
                     /gene="cysS"
                     /locus_tag="ACG8KR_002264"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HFQ0201593.1"
                     /translation="MIKIYNTLTRKKEEFIPIQKGHVGMYVCGPTVYNYIHIGNARST
                     IAFDTIRRYFEYRGYKVDFVSNFTDVDDKIIRTANELGITAPEVADRFIRAFEEDTKA
                     LNVEPATLHPRVMDHMPDILAFIQVLVEKGYAYESSGDVYYRTRKFENYGKLSDQSID
                     ELEVGASQRTGEEQQQKEDPLDFALWKQAKPGEISWDSPWGKGRPGWHIECSVMATKH
                     LGETIDIHGGGQDLEFPHHENEIAQSEAKTGKKFANYWMHNGYVTIGEDDEKMSKSLG
                     NFITVHEMIKNVDPQVLRFFMATTQYRRPIRYSETTMKEAATNLQRLKNTFDNLKFRQ
                     ETASENLADDADKIALLENLEQRFIEEMDDDFNAANGITVVYELAKWLNTYLDQPKVS
                     AAVLAQGEALFSQWLSIFGILFTAEELLDEEIEQLIEERNQARSNRDFVRSDEIRDLL
                     KEKGILLEDTPQGTRWRREA"
     gene            7056..7451
                     /locus_tag="ACG8KR_002265"
     CDS             7056..7451
                     /locus_tag="ACG8KR_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HFQ0201594.1"
                     /translation="MRDYTQLNGLALAYVGDAIYEIYVRDHLIDQGETKPNKLHRAAT
                     NYVSANAQAFLIQQMLEEHLLTEEEELYYRRGRNAKSHTSAKNADVTTYRVATGFEAL
                     MGYLHLTKQTERMEELIRWCIQKVGEKNG"
     gene            7444..8304
                     /gene="rlmB"
                     /locus_tag="ACG8KR_002266"
     CDS             7444..8304
                     /gene="rlmB"
                     /locus_tag="ACG8KR_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HFQ0201595.1"
                     /translation="MGKERTNKHQSKRGFEDKKQNRYSNKKQTGRNQSVSDQNEEALE
                     DNFVFGHHAAVEALQQGRGNKLFLQEDSRGEKVEELKEIAREQAVPVKWVPKQKLETL
                     SDHGVHQGVVLAITPYEYLSLDQLIERTKEKKEDPFFLILDSIEDPHNFGSILRTADA
                     SGVDGVIIPKHRAVGITPIVTKTSTGAVEHVPVARVTNLVQAVQQLKETGFWVFGTDM
                     TGTNFQKWNAKGSIALVIGNEGKGMSPGLKKEMDELLTIPMTGHVQSLNASVAAGLLM
                     YQAFSQRQGE"
     gene            8308..8838
                     /locus_tag="ACG8KR_002267"
     CDS             8308..8838
                     /locus_tag="ACG8KR_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HFQ0201596.1"
                     /translation="MKKQLLIVDGYNMIGSWPELVQLKKQDKMADAREALLHRLSNYA
                     KYEGMEVIVVFDAQLVPGIQQNYKKYQLDVVFTKEDETADSYIERIAGQLNDRLTQVT
                     VATSDLAEQWMIFSQGALRTSAYELYKTIKKTEKTIAQENKDMHFIDFRRNSPWNEEQ
                     LKKLSQKLDDLMRKNK"
     gene            8915..9493
                     /locus_tag="ACG8KR_002268"
     CDS             8915..9493
                     /locus_tag="ACG8KR_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFQ0201597.1"
                     /translation="MSEELLRAARNGDEEAFAALYQKYFPLLLRLKNTYYLRNYVSED
                     WEQEARIALYQALRTYEKSKGVSFGSYYKSIISNQIYSLLRKQNALKRRDQKDEVSID
                     QKIETEGPDFLAEITLQEPLAIQHLLLKEAIENCEIQFSKIEAKIFHYYMQGVDFVDM
                     AECLAMDHKQVMSAYGRVKLKIKKHLKFYNEE"
BASE COUNT         3246 a         1726 c         2140 g         2381 t
ORIGIN      
        1 aggaaaaccg gtgagaaaaa gaggatttga agttatttct aaatataaag atcaaaatat
       61 caatctaccg cagcgcgcta cacatcatgc tgctggctat gattttgaag cagcagaaga
      121 ggtcgtgatt ccaagtatct ggaaagcagg ctggaaaaag gggttggaaa cagtcgcaga
      181 tggattgaaa aaagatgtat cagaaaatga aaaacaattg aaaccaaccc tcgtgcctac
      241 tggaatcaaa gcttacatgg gagaagatga atttttgcag cttgctaacc gttcaagcaa
      301 tccgttgaag cggtttttag tgttgacaaa cggagtaggt gtgattgaca gtgattatta
      361 caataatgag gcaaatgaag gccatatcat gttccaattc accaactttg gtttcaagga
      421 tgtagtgatc aaaaaaggcg agcgtatcgg ccaagggatc tttttgccgt ttttaaaagc
      481 agatcaagat caaacgactg ctcaacgtca aggcggattt ggttcttcca gtaaataaaa
      541 cgttaaagat acttaagctt gatttaatgt ctcatgatta ggataaaagg aggcggtaca
      601 atggcaaaaa aatcaaaagt acaatttgtt tgccaaaatt gcgggtatgt gtcgcctaag
      661 ttcttgggac gctgcccaaa ttgcggaaaa tggaatacga tggtcgaaga aatcgaacaa
      721 gatacaacgg accgcagaac tcggacaagt ttgacaggag aaaaagcaaa accaacaaaa
      781 atcgcagacg tcgtccctaa aaaagaacct cggatcaaaa caaaattaga agaactgaac
      841 cgtgtgttag gcggaggtgt ggtgccaggg tctatggtct tgattggtgg cgatccaggt
      901 atcgggaaat ccactttgct gttacaagtt tctcagcaat tagcagccat aggcggaaaa
      961 gtcttatatg tatccggcga agaaagcgca gagcagataa aattacgcgc ggaacgcctc
     1021 ggaagtatca acacagaatt ttatctatac gctgaaacag atatgaacga aatcagtcgt
     1081 gccatcgaac atatctcccc tgattatgtt atcattgatt ccatccaaac gatgacgcag
     1141 ccagacatca caagtgtggc aggaagtgtc agccaagtaa gagaaaccac tgcagaacta
     1201 ctcaaaatcg ctaaaacaaa tgggatagcc atttttatcg tcggtcatgt taccaaagaa
     1261 ggctcgatcg ccggcccaag aatgttagaa catatggtgg atacggtgtt atattttgaa
     1321 ggtgaacaac atcatagttt tcggattttg cgagcagtaa aaaaccgatt tggctcaacg
     1381 aatgagattg ggatttttga aatgcatgag catggattgg aagaagtcgc taatccttca
     1441 cagattttct tagaagaacg cctagacggt gcaactggct ctgctatcgt tgtggcaatg
     1501 gaagggacac gtccaatttt ggtagagatc caagcattag tcacacctac tatgttcgga
     1561 aatgccaaac gaacaacaac aggtcttgat ttcaatcggg tatctctgat catggcagtg
     1621 ctggaaaaaa gagctgggtt gcttcttcag aatcaagatg cttacttaaa agctgcaggt
     1681 ggggtaaaat taaatgaacc agcgattgat ttagctatcg ctgtcagtat tgcttcaagc
     1741 tataaagaaa aaggaacaca gccaacggaa tgtttcatcg gtgaaattgg actgactgga
     1801 gaaatcagac gtgtcagtca tattgaacaa cgagtcaaag aagtccaaaa actcggcttc
     1861 acaaaagtat acctacctaa aaacaactta ggtaactggg aagcgccaaa aggaatcgag
     1921 atagttggag tagcaacttt ggctgaaacg ttgaagcgtg tgttccgcta actaataaat
     1981 gattgtcgaa acggaggaaa aacgatgcaa aaacgtgtca ttactttgct gatgatcatt
     2041 gtcggcgcaa gcttgggtat ttcattttta ccctcagctt ggcaggcaat cggtcaacag
     2101 tcaaacggat ggctgaataa tacatttacc aacagcctgt taggcgcact tattttcttt
     2161 ttattatctt tgctgttggc caaacatatc tcagctatta tcaaacaagt agaacaaaaa
     2221 ctaagtgagg tcagcttgac ttatcttctc ttcggtgcga tcggtgcgat catcggattg
     2281 actttaggtg ttgtgatctc gacccctctt tacaatatgg aaattccatt cgtcaatagt
     2341 gtattgccga ttttactgat gatcatctta ggctatctgg gattgagaat gggaacgaca
     2401 cgaatcgaag aatggaagaa agtttttggt tcacgaagca aaaaaatcga gcaagaaaca
     2461 gctagtcaag ttgagagtca actgttagaa cgaaaatcag gagaaaattt ccataaatat
     2521 aaaatcttgg atacaagcgt gatcattgac ggtcggattt atgatattac aaagactgga
     2581 ttcttagaag gaaccttgat gatccctaat tttgttcttt acgaactaca atacatcgcg
     2641 gactcagggg atagtttgaa acgtgtgcgt ggtcgtcgcg gattagatat cctaaatgcc
     2701 ttgcaaaaag aagagggtat atctgtcgaa atgtacgatg gagactttga agatatcagt
     2761 gaagtagaca gcaaattgat caaattggct aagcttttag atggtgtcat tgtaaccaat
     2821 gactataact tgaataaagt ctctgaattc caaaatgtac ctgttcttaa tatcaatgcg
     2881 ttggcaaatg ccgtaaaacc agtcgtcatc cctggtgaaa cgatgaatgt tctagtagta
     2941 aaagccggga cagaacgcca acaaggagta gcctaccttg atgacggcac gatggttgtc
     3001 gtagaagatg gacagcatta catgaatgaa cgtatcgaag tcgttgtaac aagtgccctt
     3061 caaacagctg ctggacgcat gatctttgct aaacctgcac atgcaggacg aggaatcgac
     3121 aataaaaaag agacagagaa aaagactaaa caatcaaaat aaaaaagaaa ttcagccaac
     3181 tggtaaggaa attcaaccag ttggctttac tatttacctt agaaaaggta aaatttgtgc
     3241 tgagtggctt taaataaatg atatttgaga agccgtaact gacggaattt ttgctgttag
     3301 tcaaagtcta gtttgaaaga agaggaagaa aatgacgaaa gtacgcgtac gctatgcacc
     3361 aagtccaact gggcacctgc atattggaaa tgcaagaact gcattattca attatttgtt
     3421 tgcccgccac aatgatggag actttattat tcgtattgag gatactgacc aaaaacgtaa
     3481 tatcgaagat ggcgaaaaaa gccagttgga aaaccttgct tggttgggaa tgaattggga
     3541 tgaatctcct gaaaatcctg gagaatacgg cccttaccgt caatctgaac gtaaagaaat
     3601 ctatcaaccg ctgattgatc agctgcttgc tagcaatcga gcatacaaat gctattgcac
     3661 agaagaagaa ttagaagcag aacgggaagc acaacgcgca agaggagaaa tgcctcatta
     3721 cgcaggaaca tgtgcgaact tgacacctga agaacaagca gaaaaagaag cacaaggtct
     3781 agaatctgtt gtacgtttcc gtgtgcctcg caatacagaa tacgcattta cagatatggt
     3841 gaaaggcgct atttcgttcg aatccgataa tatcggtggc gactttgtca tacaaaaacg
     3901 cgatggtatg ccaacttata attttgctgt tgcggtcgat gatcatttga tgaaaatcac
     3961 tcacgtttta cgtggagatg accatattgc caatacacca aaacaactga tgatctatga
     4021 agcatttggc tggacaccac caaccttcgg acatatgaca ttgatcatca acagtgaaac
     4081 aggtaaaaaa ctaagcaaac gtgacgaatc aatcttgcaa tttatcgaac aatatcgcga
     4141 attaggctat ctaccagatg caatgttcaa ctttattgca ttattgggct ggtcacctgt
     4201 gggcgaagac gaaatcttcg aacaccaaga attgatcaaa atgttcgatc caaaacgcct
     4261 aagtaaatca cccgcagctt tcgatgcgaa aaaactgcaa tgggtaaaca accattacat
     4321 caaagcaatg gatctagatg catttactga catgtgtctg ccttatctaa tcgctgatgg
     4381 acgtgtagca gaaaacccag atgaaaagac aatcgaatgg gtgaagaaga tcgttagcct
     4441 gtatcaacct caaatgagct atgctgcaga aatcgttgat ctgtctacgc tatttttcaa
     4501 tgaacatcct gtattgaatg aagcagcaaa agaagtactt gctggtgaaa cagttcctac
     4561 tgttctccac gcattcaaag cacagctaga acaaatggaa gtagtagatg ctccaactgt
     4621 caaagctgcc atcaaagctg ttcaaaaaga aactggcgta aaaggaaaaa atcttttcat
     4681 gccaatccgt gtagctgtat caggacaaat gcatggtcca gaattgcctg aaacgatcga
     4741 attgcttggt cgtgaaaaat cattagcaca tttaaatcaa gtactgaact aaacacgatg
     4801 aacagaaaaa agtaagcagc tgatcattac aagagagata ccgggtggtg caaggtatca
     4861 atgaatggtt gccgaaatgc ctctgggagt ggtcttggcg aaacacagaa aacaggaaaa
     4921 actgttttat aggaaacaaa gtagccaaga acgtataaca ggcgttagct gtcagagata
     4981 atcattgatt atgaagaaaa gtggaaccac gcggaaagcg tcttttgata aggaaactta
     5041 tttaaaaggc gcttcttttc tttataagaa gatgatttcc tacataaaat tcttacgaaa
     5101 ggagtggaaa gtcatgagtt ggtttacccg agcgattgat gcagcaaaga gaaacgatcc
     5161 ggctgcacgt tcgacagctg agatcgtttt gacataccct ggttttcatg ccttgttttg
     5221 gcatcgtttc tctcattttt tatatcagca ccggctcttt ttattggcga agatcaatgc
     5281 tcaattctgg cgtttcctaa ctggtgttga gatccatcca ggagcaacga ttggaactgg
     5341 cgtgttcatc gaccatggaa tgggaatcgt cattggcgaa accgcagaaa tcgaggacga
     5401 tgtgatttta ttccatggcg taacattagg cggcacagga aaagaaacgg gtaaaagaca
     5461 cccaactgtt aaacaaggag ccatgctctc tgcgaatgcg cagatattag gaccggtaac
     5521 gatcggaaaa aacgccaaaa tcggtgcagg tgccgttgtg ttgaaagatg ttccagacga
     5581 cgctacagcg gtaggagtac cagcaaaagt cgttcgaatc aaaggagaaa aagtgaggag
     5641 atcagaatga taaaaatcta caatacattg accagaaaaa aagaagaatt catccctata
     5701 caaaagggac atgtcggtat gtatgtctgc ggaccgactg tgtataatta tatccatatc
     5761 ggaaatgctc gtagcaccat tgctttcgat acaattcgac gctattttga atatcgaggc
     5821 tataaagtcg atttcgtatc gaatttcacg gatgtagacg ataaaatcat ccgtacagca
     5881 aacgaattag gtattacagc accagaagtt gctgatcgtt tcatccgagc atttgaagaa
     5941 gatacgaaag cgttgaatgt cgagcctgct acactgcatc caagagtcat ggatcatatg
     6001 ccagatattt tagcatttat tcaagtgttg gttgaaaaag gctatgctta tgaatcttca
     6061 ggtgatgtgt attaccgtac ccgtaaattt gaaaattatg ggaaattaag tgaccaatcc
     6121 atcgatgaac tggaagtcgg agcaagtcag cgtacagggg aagaacagca gcaaaaagaa
     6181 gacccattag atttcgcttt atggaaacaa gcaaaacctg gcgagatttc ttgggattca
     6241 ccatggggca aaggacgtcc cggttggcat attgaatgtt ccgttatggc aacaaaacat
     6301 ttaggagaaa cgatcgatat ccatggtggc gggcaagatt tagaatttcc tcatcatgaa
     6361 aatgaaatcg cacaaagtga agcgaaaaca gggaaaaaat tcgctaatta ctggatgcac
     6421 aatggctatg taacgattgg cgaagacgat gaaaaaatga gtaaatctct agggaatttc
     6481 atcactgtac atgaaatgat caaaaacgtt gatccacaag tcttgcgttt cttcatggcg
     6541 accacacaat accgtcgtcc gatccgctac agtgaaacga cgatgaaaga agcagccacc
     6601 aatttacaac gactgaagaa tacgtttgac aatttgaaat tccgccaaga aactgcctca
     6661 gagaatttgg cagatgatgc ggataaaatc gctttgttag aaaatttaga acagcgattc
     6721 atcgaagaaa tggacgatga ttttaatgca gcaaatggga tcacagtggt ctatgagctt
     6781 gctaagtggc tgaacactta tctagaccag ccgaaagttt ctgccgctgt cttggcacaa
     6841 ggagaagctt tattctcgca atggctttcg atcttcggta ttttatttac agcagaagag
     6901 ttattagatg aagagattga acaattgatc gaagaacgaa atcaagcaag aagcaatcgt
     6961 gattttgtcc gaagcgacga aattcgtgat ttgttgaaag aaaaagggat cttattagaa
     7021 gatacaccgc aaggaacccg atggagaaga gaagcatgag agattacaca caattgaacg
     7081 gtttagcctt ggcttatgtt ggagatgcga tttacgaaat ctatgttcgt gaccacctga
     7141 ttgatcaagg agaaacaaaa cccaataagc tgcatcgtgc ggcaacgaat tatgtttctg
     7201 cgaatgcaca agcctttttg attcagcaaa tgctggaaga acatcttttg acagaagaag
     7261 aagaacttta ttatcgcaga ggaagaaatg ctaaaagcca tacgagcgca aaaaatgctg
     7321 atgttacgac gtatcgggtc gcaacagggt tcgaagcgct catgggctat ctgcatttga
     7381 caaaacaaac cgagcggatg gaagaattga tccgctggtg tattcagaaa gttggtgaaa
     7441 aaaatgggta aagaaagaac aaacaaacat caatcgaaac gtggatttga agacaaaaaa
     7501 caaaatagat attcaaataa aaagcaaact ggacgaaatc agtcagtttc tgatcaaaat
     7561 gaagaagcat tagaagataa tttcgtcttt gggcaccatg ccgcagtaga agcgctacaa
     7621 caagggcgag ggaataaact ctttcttcaa gaagattcac gaggagaaaa agtagaagaa
     7681 ctgaaagaaa tcgcacgtga acaagctgtg ccagtcaaat gggttcctaa acaaaaatta
     7741 gaaactttga gcgatcacgg cgtgcatcaa ggagtcgttc ttgcgattac accttatgaa
     7801 tatttatccc ttgaccaact gattgaacga acaaaagaga aaaaagaaga ccccttcttt
     7861 ttgattttgg atagcatcga agatccacac aattttgggt cgattttacg gacagcagac
     7921 gcaagtggcg tcgatggtgt gatcatacca aaacaccgag cagttggaat cacacctatc
     7981 gtaacaaaga cttccactgg ggcagtcgaa catgtccctg ttgcacgcgt gacgaatttg
     8041 gtacaagctg tgcaacagct caaagaaaca ggattttggg tatttggcac agatatgacc
     8101 ggcacaaact tccaaaaatg gaatgccaaa ggatcaattg ctttagtcat tggtaatgaa
     8161 ggcaaaggaa tgagtccagg attgaaaaaa gaaatggatg aattgctgac gatcccgatg
     8221 accgggcacg tccagagctt aaatgccagt gttgctgctg gtttgcttat gtatcaggct
     8281 ttttcccaac gtcaggggga gtagaccatg aaaaaacagc tgttgattgt agacggctac
     8341 aacatgattg gttcgtggcc ggaacttgtc cagcttaaaa agcaggacaa aatggcggat
     8401 gcaagagaag ctcttttaca tcgtttatca aattatgcga aatatgaagg aatggaagtc
     8461 atcgttgtct tcgatgccca actagttccg ggcatccaac aaaattataa gaaataccag
     8521 ttggatgttg tttttacgaa agaagacgaa acggctgaca gttatatcga gagaatcgct
     8581 ggtcagctga atgaccgttt gacacaagtg acagtggcga caagcgattt agcagaacag
     8641 tggatgatct tttcccaagg agcattacga acttctgctt atgaattata taagacgata
     8701 aaaaaaacgg aaaaaacaat agcccaagaa aataaagata tgcattttat tgatttccga
     8761 cgtaattctc catggaatga agagcagtta aaaaaattgt cccaaaaatt ggacgattta
     8821 atgagaaaaa ataaataaca tatcgttcta atcagttcga tgaatcattg atgataagat
     8881 tcctccataa acgaatttat ggaggaatta ttttatgtct gaggagttat tgcgcgctgc
     8941 acgtaatggt gacgaagaag catttgctgc cctgtatcaa aaatattttc cacttttact
     9001 acgtttgaaa aacacctatt atttaagaaa ttatgtttct gaggattggg aacaagaagc
     9061 aaggatcgct ttatatcagg cattgagaac ttacgaaaaa agcaaaggtg ttagctttgg
     9121 aagttattat aaaagcatta taagcaatca aatctattct ctgttgagga agcagaacgc
     9181 gctgaagcgt cgtgatcaaa aagatgaagt ctcaatcgac caaaaaatag agacagaagg
     9241 accagacttt ttagcagaga tcacattgca agaaccttta gcgattcagc atcttttatt
     9301 aaaagaagca atcgaaaatt gcgaaatcca gttttcaaaa atagaagcta aaatttttca
     9361 ttattatatg caaggcgttg attttgtaga catggctgaa tgtttagcaa tggatcacaa
     9421 gcaagtaatg agcgcttacg ggagagtaaa actcaaaata aaaaaacact tgaaatttta
     9481 taatgaggaa taa
//
LOCUS       DAZKLD010000114         9481 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.114, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000114 DAZKLD010000000
VERSION     DAZKLD010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9481)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9481
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.114"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(81..1532)
                     /gene="cls"
                     /locus_tag="ACG8KR_002269"
     CDS             complement(81..1532)
                     /gene="cls"
                     /locus_tag="ACG8KR_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HFQ0201598.1"
                     /translation="MVSSIITALYLLNALIALVAILIKPRDVAAIWAWLLVLFALPGV
                     GFVLYLFFGRGLTDKKKFYLRQSDLKELENFQSFRGDTIEHYDPDMGDKDKQQFVDFF
                     SSLNRMPLTRMNSVTLLTDGQEKLDSLLQDLKKAKHSIHIEYYAFVTDNIGQQVLHVL
                     EEKAAEGVEVRILYDAFGSHGTKAKDFNRLIKNGGHVHTFVTSQRALLRFRLNYHDHR
                     KIVVIDGKISYTGGFNIANQYVNTTKKFGYWRDTHIRIFGAASSLLQLRFLTDWNVSV
                     PEEKKVGYHLNYFFKKADRDESKLADTSIQLVSSGPNNEREQIKLSFIKLITSAKKRV
                     WIQTPYLVPDESVIAALKIATASGVDVKIMIPNKPDHPFIYRATQYYARQLIKENVQI
                     LVYENGFLHAKTLIMDDEICMVGSANQDIRSYRLNFETSAVIYDPEFLEELATQFKED
                     ETHCSSMTTETVKEMSNWLLFKQQISRLFSPIL"
     gene            1873..4539
                     /locus_tag="ACG8KR_002270"
     CDS             1873..4539
                     /locus_tag="ACG8KR_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFQ0201599.1"
                     /translation="MENKEQAQQAVYKETIDSLMQQTQSSTDGLSTTQAKEKLERDGL
                     NQLKEKPKKPTWKLFLETFKDAMVIVLLIVALIQMVMGDYIEFLVIFAVLMLNSIISV
                     VQTKKAEGSLEALKNLSAPDARVIRDGKEKTIPANELVVGDIVLLDAGDYVPADGRLL
                     DAGSLKVDEGMLTGESTSAEKEVTDITSTVPIGDRTNMVFSGTIVTYGRGKFLVTSTG
                     NNTEIGQVAGLLESTTEQVTPLQRGLDKFSKKLSLAILALSLLILGIQLFRIYLGEGT
                     GDMTADIVSAVMFAVAVAVAAIPEALQSIVTIVLSLGTNKMAKRHAIIRKLPAVETLG
                     STSIICTDKTGTLTQNKMTVVDYFLGNGDTGDFTNDPSKWTADERRLIEISVLANDAS
                     ISEDGTKLGDPTEVAFIDFSEKLNQPYQEIRNNYPRQAELPFDSDRKLMSTGHTIDNK
                     NYLFVKGGPDIVFQRSKKVLLNGEVVPMTDELMKKFQEQNEAFSTRALRVLAFAYRPL
                     DNFDLSFEDEDDLILVGLLAMIDPPRAEVTQAVTEARSAGIKTIMITGDHKTTAVAIA
                     KQIGIFEEGNLALTGTELDELTEDQLNEVLEKVTVYARVSPENKIRIVRAWQNKGHIS
                     AMTGDGVNDAPALKQADIGIAMGTGTDVAKDAAAMVLTDDNFASIVSAVEVGRNVFDN
                     IKKAISYLFAGNLGAIIAIIFALVADWSNPFTALQLLFINLVNDSLPAIALGTEKAEP
                     ATMNRPPRDPNSGIFTDKTLISVTYRGVLIGIAVIFAQWLGMQHSPEFGVAMSFSTLI
                     WCRTLQTFPARSNTQTAIQAGFFSNRSVLLAVVVCSALYCLTMIPGLKEVFSIPSAFG
                     LTEVFTCMGIALVTIVLMEITKLIIVRVQGNK"
     gene            4903..5655
                     /locus_tag="ACG8KR_002271"
     CDS             4903..5655
                     /locus_tag="ACG8KR_002271"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFQ0201600.1"
                     /translation="MNEKEWDEFAEEYYEIQQESQVTIAEDVKNYLKTEKVLPAETAA
                     DVAGGSGRFLSLAKEINRYDLIDFSSGMLENAKKEAEKNELPNVSFIKQSLEEFIQSN
                     ETYEMVFTAANPALVHLIQLEKLMKKSSKWCVIIRVVESIDDVFYPIEKQLGMIEEDP
                     NTSPVVMDQFETYLIDEGFKVKKKDFTYFTQESITEELMKLYYDAYAKDPVVTRYIRK
                     LFSEKREILSTTKLTYRLLLWSADKKRTFHDS"
     gene            complement(5677..6831)
                     /locus_tag="ACG8KR_002272"
     CDS             complement(5677..6831)
                     /locus_tag="ACG8KR_002272"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004421 - hydroxymethylglutaryl-CoA
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HFQ0201601.1"
                     /translation="MKIGIDRLSFFIPNLYLDMTELAESRGDDPAKYHIGIGQDQMAV
                     NRANEDIITLGANAASKIVTEKDRELIDMVIVGTESGIDHSKASAVIIHHLLKIQSFA
                     RSFEVKEACYGGTAALHMAKEYVKNHPERKVLVIASDIARYGLASGGEVTQGVGAVAM
                     MITQNPRILSIEDDSVFLTEDIYDFWRPDYSEFPVVDGPLSNSTYIESFQKVWNRHKE
                     LSGRGLEDYQAIAFHIPYTKMGKKALQSVLDQTDEENQERLMARYEESIRYSRRIGNL
                     YTGSLYLGLTSLLENSKSLQPGDRIGLFSYGSGAVSEFFTGYLEENYQEYLFAQSHQE
                     MLDSRTRITVDEYETIFSETLPEHGECAEYTSDVPFSITKIENDIRYYKI"
     gene            7016..9457
                     /locus_tag="ACG8KR_002273"
     CDS             7016..9457
                     /locus_tag="ACG8KR_002273"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004420 - hydroxymethylglutaryl-CoA
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0015936 - coenzyme A metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HFQ0201602.1"
                     /translation="MKEVVMIDAARTPIGKYRGSLSPFTAVELGTLVTKGLLDKTKLK
                     KDKIDQVIFGNVLQAGNGQNVARQIALNSGLPVDVPAMTINEVCGSGMKAVILARQLI
                     QLGEAELVIAGGTESMSQAPMLKQYQSETNEYGEPISSMVNDGLTDAFSNAHMGLTAE
                     NVATQFSVSREEQDRYALSSQLKAAHAVEAGVFSEEIIPVKISDEDVLSEDEAVRGNS
                     TLEKLGTLRTVFSEEGTVTAGNASPLNDGASVVILASKEYAENNNLPYLATIKEVAEV
                     GIDPSIMGIAPIKAIQKLTDRSGMNLSTIDLFEINEAFAASSIVVSQELQLDEEKVNI
                     YGGAIALGHPIGASGARILTTLAYGLLREQKRYGIASLCIGGGLGLAVLLEANMEQTH
                     KDVQKKKFYQLTPSERRSQLIEKNVLTQETALIFQEQTLSEELSDHMIENQVSEVEIP
                     MGIAQNFQINGKKKWIPMATEEPSVIAAASNGAKICGNICAETPQRLMRGQIVLSGKS
                     EYQAVINAVNHRKEELILCANESYPSIVKRGGGVQDISTREFMGSFHAYLSIDFLVDV
                     KDAMGANMINSILESVANKLREWFPEEEILFSILSNFATESLASACCEIPFERLGRNK
                     EIGEQIAKKIQQAGEYAKLDPYRAATHNKGIMNGIEAVVAATGNDTRAVSASIHAYAA
                     RNGLYQGLTDWQIKGDKLVGKLTVPLAVATVGGASNILPKAKASLAMLDIDSAKELAQ
                     VIAAVGLAQNLAALRALVTEGIQKGHMGLQARSLAISIGAIGEEIEQVAKKLREAEKM
                     NQQTAIQILEKIREK"
BASE COUNT         3065 a         1707 c         2027 g         2682 t
ORIGIN      
        1 ttcggaagaa gggactgtga caagattttt gtcacagtcc ctttaatctt agtatatttt
       61 tctctacttg aataaaaaaa ttataggatt ggagaaaata atcgagaaat ttgttgcttg
      121 aataatagcc agttagacat ttccttgact gtttcagttg tcatggatga acaatgtgtc
      181 tcatcttctt tgaactgagt agcaagttct tctaaaaact caggatcgta aatgacagca
      241 cttgtttcaa aattcaatcg gtagctacga atatcttgat ttgctgaacc taccatgcag
      301 atttcatcat ccattatcaa tgtttttgca tggaggaagc cgttctcata gacaaggatt
      361 tgtacatttt ccttgatcag ctgccgagca taatattgtg ttgctcgata aataaaagga
      421 tgatccggtt tgttgggaat catgattttc acatctacac cagaggcagt tgcgattttt
      481 aaagcagcaa tgacactttc atcaggaaca aggtaaggtg tctgtatcca aacacgtttt
      541 ttagcagaag taatcaattt gataaatgaa agcttgattt gttccctttc gttattcggt
      601 ccgcttgaaa caagctggat ggatgtatca gcaagcttag attcatctcg atctgctttt
      661 ttaaagaaat aattcaaatg atagccgacc tttttttctt caggtaccga gacgttccag
      721 tctgttaaga agcgaagctg gagcaaagaa gaagcggcac cgaaaatccg tatatgcgta
      781 tcgcgccaat agccgaattt ttttgttgta tttacatatt gattggcaat attgaaacca
      841 ccggtataac taatctttcc atcgatcaca acgatttttc ggtgatcatg gtaattcaat
      901 cggaaacgaa gtaatgccct ttgtgaggta acaaatgtat ggacatgtcc accatttttg
      961 attagacgat tgaaatcttt tgcttttgtg ccatgagagc caaatgcatc atataatatt
     1021 cgaacttcca cgccttctgc ggctttttct tctaaaacat gtaagacttg ctggccgata
     1081 ttatctgtca caaatgcgta atattcgata tggatcgaat gtttggcttt ttttagatct
     1141 tgaagcagtg aatccaattt ctcttgtccg tctgtgagaa gagtgacaga attcattctt
     1201 gtcagcggca tacgatttaa tgaagagaag aagtcaacaa attgctgttt gtctttatcg
     1261 cccatgtcag gatcgtaatg ttcaatggta tctcctctaa aagactgaaa gttttctaat
     1321 tctttcaagt cactttgtcg gagatagaat tttttcttat ccgttaatcc acgtccgaaa
     1381 aataaatata atacaaagcc caccccagga agggcaaaca atactaagag ccatgcccaa
     1441 atggctgcta catctcgggg tttgatcaaa atagccacca aagcaataag tgcatttaat
     1501 agataaaggg cggttataat actagatacc acaaaaatgt tcctccttac atacaataac
     1561 attagtatag catgatagaa gaaagaagcc taccaaggtg aatatacttt tcactccttt
     1621 catgaaaatg taggtaaatc gggctaaagt agttgctttt tgtaggttaa taaggtagga
     1681 tattagtaca gttgaatatc tccttagggg agtaaccggc aacgtttgtt gcgataatat
     1741 caacagtaag ttaaccaaat ggttttctgg tattgtatga aatggtgaga cctaagacaa
     1801 atgcgtattt gtcttaggtc tctttttttt cagacaggac tagggccaag atcaaaaaat
     1861 gggggatgaa atttggaaaa caaggaacaa gcacaacaag cggtctataa agaaacgatc
     1921 gactcgttga tgcagcaaac gcagagctca accgatggat tgagcaccac gcaagcgaag
     1981 gaaaaattag aaagagatgg cttgaaccag ctaaaagaaa agccgaagaa gccaacttgg
     2041 aaattgttct tagaaacttt caaagatgca atggttatcg tattgctgat tgtcgcactg
     2101 attcagatgg tcatgggtga ctacatcgaa ttcttagtta tttttgcagt attgatgttg
     2161 aattctataa tcagtgttgt ccaaacaaaa aaagcagaag gttctttaga agcgttgaaa
     2221 aacctttcag cgcccgatgc tcgagtgatt cgtgatggta aggaaaaaac gattccggca
     2281 aacgaacttg tggtaggaga tatcgtgttg ttagacgcag gcgattatgt acctgctgat
     2341 ggacgtttac tagatgcggg atcattaaaa gtagatgaag gtatgctgac aggagagtcg
     2401 acttctgcag aaaaagaagt aacggatatc acaagtacgg taccaatcgg agatcgtaca
     2461 aatatggtat tcagtggaac gatcgtaact tacggacgcg gtaaattctt agtgacttca
     2521 actgggaata atacggagat cggacaagtt gctggccttt tggaatcaac aactgagcaa
     2581 gtcactccgt tacaacgagg tctggataaa ttcagtaaaa aactaagttt ggctattttg
     2641 gcattgtctt tattgatttt aggtatccaa ctgtttcgta tttatcttgg agaaggtaca
     2701 ggagatatga ctgcagatat cgtcagcgca gtgatgtttg ccgtagccgt agccgtagct
     2761 gctattccag aagcattaca gtctatcgta acaatcgtgt tatctttggg aacgaataaa
     2821 atggcaaaac gtcatgccat tattcggaag cttccagcag ttgaaacact tggttcgaca
     2881 agtatcatct gtacagataa gactggtaca ttgacacaaa acaaaatgac agttgttgac
     2941 tactttttag gtaatggcga tactggggat ttcacaaatg acccttcaaa atggacagct
     3001 gacgaacgtc gcttgatcga aatcagtgtt ttagcgaatg atgcatcgat cagtgaagac
     3061 ggtactaaat tgggcgaccc tacggaagta gcgtttatcg actttagtga aaaattaaac
     3121 cagccttatc aggaaatccg aaacaactac ccaagacaag cagaacttcc ttttgactca
     3181 gatcgaaaat taatgtctac tggacacaca attgataaca aaaattactt gtttgttaaa
     3241 ggcggaccag atatcgtctt ccaacgcagt aaaaaagtat tgctaaatgg tgaagtcgtt
     3301 ccgatgacgg atgaattgat gaaaaaattc caagagcaaa atgaagcttt ttcaacacgc
     3361 gcattacgtg tactagcttt tgcttaccgt ccacttgata attttgattt atcatttgaa
     3421 gatgaagacg atttgatcct tgttggtttg cttgctatga tcgatcctcc acgtgctgaa
     3481 gtgactcagg cggtaacaga agcaagaagt gcaggaatca aaacaatcat gattactggt
     3541 gaccacaaaa caacagctgt tgctatcgca aaacaaatcg ggatttttga agaaggaaac
     3601 ctggctttga ccgggacgga actagatgaa ttgacagaag atcaactaaa cgaagtttta
     3661 gaaaaagtca ctgtttatgc acgtgtttca cctgagaata aaattcgtat cgttcgtgct
     3721 tggcaaaata aaggtcatat ctcagctatg acaggtgatg gtgtaaatga cgcaccagca
     3781 ttgaaacaag cagacatcgg aattgctatg ggaacgggta cagatgtggc aaaagatgca
     3841 gcagcgatgg tattgacgga tgataacttt gcttctattg tcagcgcagt agaagtagga
     3901 cgtaatgtgt ttgataatat caagaaagct atttcttatc tatttgcagg aaatttaggt
     3961 gcgatcattg cgatcatctt tgccttagtt gctgactggt ccaatccatt tacagcatta
     4021 caactgttgt tcatcaattt agtcaatgat tcactgccag caattgcatt aggtacagaa
     4081 aaagctgaac ctgctacgat gaaccgcccg ccaagagatc caaattcagg aatctttacg
     4141 gacaaaacat tgatttctgt aacgtatcgt ggtgtgctga ttgggattgc tgttatcttt
     4201 gctcaatggc ttggcatgca gcattcacct gaattcggtg tagccatgtc attttcaaca
     4261 ttgatttggt gtcggacatt gcaaacattc ccagcacgtt caaacacaca aacagctatc
     4321 caagcagggt tcttctctaa cagaagtgtc ttactagctg tagttgtatg tagcgcgctt
     4381 tactgcttaa caatgatccc aggactgaaa gaagtcttct ctatcccatc agcatttgga
     4441 ttgacagaag tctttacatg tatgggaatc gcacttgtga caattgtttt gatggaaatc
     4501 actaaattga tcattgtacg agtacaagga aataaataaa acgaatcaac gaggttgtga
     4561 gcctgccgtt taattctgag tcacaaggtg caatttcaac aaacagcttc tcatgtttgt
     4621 tgaatattgt gacttgtgac cgaagagttg gcaggagaac accgtttaag gaagaagttg
     4681 taaaatcagc attttgagct aatactgagg gtcatctgat tggacactgt ttattcggaa
     4741 agagggactt tgacacgact ttggtcacag tccctttttt tatttatgat agacggaaag
     4801 ccaatcattt tattattcat caaaagagat agaatagata aaagaggaga atgatttatt
     4861 ccgaaaagtg aaaacatata acaaaaaatt agaggaaatc aaatgaatga aaaagaatgg
     4921 gatgaatttg ctgaagaata ttatgaaatc cagcaagaat cgcaagtaac tattgctgaa
     4981 gatgtaaaaa attatctgaa aacggagaaa gtcctgcctg ctgaaacagc agcagacgtt
     5041 gctggaggat ctggcagatt tctttcctta gcaaaagaaa ttaatcgcta tgatttgatc
     5101 gatttttctt caggaatgct agaaaatgca aaaaaagaag cagaaaaaaa tgagttgcca
     5161 aatgtttcct ttatcaaaca gtcgttagaa gaatttatcc aatcaaatga gacatatgaa
     5221 atggttttta cagcagcaaa tccagcattg gtacatctta ttcaattaga aaaattgatg
     5281 aaaaagtcaa gcaaatggtg cgtgataatc agagtagtgg aatcaatcga tgatgttttt
     5341 tatcctatcg aaaagcaact aggaatgata gaagaagatc ctaatacatc gccagttgta
     5401 atggatcaat ttgaaacata cttaattgat gaaggattca aagtgaagaa aaaagatttt
     5461 acttatttta cacaggaatc aattactgaa gaattgatga agctatacta tgacgcgtac
     5521 gcaaaagatc ctgttgtgac caggtacatt cgcaaactat tcagtgaaaa acgagaaatc
     5581 cttagtacga ctaagctgac ctatagactg ctcctttggt ccgcagataa aaaaagaacc
     5641 tttcacgact cgtgaagggt tctttttatg attttattat attttgtagt aacgaatatc
     5701 attttcgatc tttgtaatcg aaaaaggtac atctgaagta tattctgcac attctccgtg
     5761 ttcaggaagc gtctcactga aaatcgtttc atattcgtca acagtgatcc gtgttcgaga
     5821 atctagcatc tcttgatgag attgtgcgaa gagatattct tgatagtttt cttccagata
     5881 acctgtaaaa aattcactta ctgcaccaga accgtagctg aatagaccga tgcgatctcc
     5941 tggctgtagt gatttcgaat tttccagtag agaagttagc cccaggtata atgaaccagt
     6001 gtaaagatta ccgattcgtc ggctgtaacg gatgctttct tcatagcgag ccataagacg
     6061 ttcctgattt tcttcgtctg tttggtctaa tacgctttgc aatgcctttt ttcccatctt
     6121 agtatacgga atatggaaag caatcgcctg ataatcttcg agtcctcgac ccgacaattc
     6181 tttatgtcga ttccaaactt tttggaatga ttcgatatac gtagaattag ataaaggacc
     6241 atcaacaaca ggaaattcgc tataatctgg acgccagaaa tcatagatat cttctgtcag
     6301 aaatacgctg tcgtcttcaa tcgataaaat acgcgggttt tgagtgatca tcatcgcaac
     6361 agcaccgaca ccttgcgtca cttcaccacc gcttgccaag ccgtaacgag caatatcact
     6421 tgctatgact agtacttttc gttctggatg atttttgaca tattctttcg ccatgtgcaa
     6481 agctgcggtg ccaccgtagc aagcctcttt tacttcaaaa gaacgagcaa aagattggat
     6541 tttcagtaaa tggtggataa ttaccgcact cgcttttgaa tgatcaatcc cggattctgt
     6601 tccgacgatg accatgtcga ttagctcacg gtctttttct gttacgattt tgctggcagc
     6661 atttgctcct agtgtaatga tgtcttcatt tgcacgattg actgccattt ggtcttgacc
     6721 aatccctata tggtattttg caggatcatc cccacggctt tctgccaact ctgtcatatc
     6781 taaatataaa ttaggaataa aaaaggaaag acgatcaatc cctattttca tttcaacacc
     6841 tctttactta tgataaaaaa cttactttac taaggtatca taaaaagatt aaaagaaata
     6901 taaaaaagag aatatttaag gattgttaaa atttaatctt agaaaaaaat aactggaaca
     6961 agagctattt gtttttacag caaagaatgg tatatttttg taggaggtgg cttacttgaa
     7021 agaagtcgtt atgatagatg ctgcaagaac gccgatagga aagtatcgcg gaagtttaag
     7081 tccatttact gcagttgaat taggcacact tgtgactaaa ggattattag ataaaacaaa
     7141 actgaaaaaa gataaaatcg atcaagtaat atttggaaat gtcctgcaag ctggtaatgg
     7201 tcaaaatgtt gccagacaga ttgctttgaa cagcggtctc cctgtagatg tcccagcaat
     7261 gacgatcaat gaagtttgcg gatctggtat gaaagcagtg atcttagcac gccagctgat
     7321 ccaattaggt gaagcagaac ttgtgatagc cggcggaaca gagagtatgt ctcaagcacc
     7381 tatgctgaaa cagtatcagt cagaaacaaa tgaatatggt gaaccaattt ccagtatggt
     7441 caacgacgga ttgactgacg cattttcaaa tgcacatatg ggattaaccg cagagaatgt
     7501 tgcaacacaa ttttctgtga gcagagaaga gcaggatcgc tatgccttgt cgtcccagtt
     7561 gaaagcagca catgctgtcg aagccggtgt attttctgag gagatcatcc cagtcaagat
     7621 ttctgatgaa gacgtgttat ctgaggatga agcagttcgt ggaaatagta cattggaaaa
     7681 actgggcacg ttacgtacag tattctcaga agaaggaact gtaacagcag gaaatgcttc
     7741 cccgttgaat gacggtgcct ctgtggtgat ccttgcatcc aaagaatacg cagaaaataa
     7801 taatctgcct tatttagcaa ccatcaaaga agtagcggag gtcggtattg acccaagtat
     7861 catgggaatc gctccaatca aagccattca aaaattgaca gatagatcag gaatgaattt
     7921 atcaacaatc gatctttttg agatcaatga agcatttgca gcttcttcta tcgtagttag
     7981 ccaagaactg cagttagatg aagaaaaggt caatatatat ggaggagcaa tcgcattagg
     8041 ccatccaatt ggtgcaagcg gtgcacgtat cttgactaca ttagcttacg gattgcttcg
     8101 tgaacagaag agatacggta ttgcttcatt atgtattggt ggaggtttag gcttggcagt
     8161 cttgttagaa gcgaatatgg agcaaacaca taaggatgtg caaaaaaaaa agttttatca
     8221 gcttacgcca tctgaacggc gcagtcaatt aatagaaaaa aatgtattga cacaagaaac
     8281 tgcgttgatt tttcaggaac aaacactttc tgaagagctc tccgatcaca tgatcgaaaa
     8341 ccaagtgagt gaagtggaaa tcccaatggg gatcgcgcag aattttcaaa tcaatggcaa
     8401 gaaaaaatgg attccgatgg caacagagga accatctgtc attgcagctg caagtaatgg
     8461 tgcaaaaatc tgtggaaata tctgcgcaga aacacctcag cggctgatgc gagggcaaat
     8521 cgttctatca ggaaaatcgg agtatcaagc agtgatcaat gcagttaatc atcgaaaaga
     8581 agagctgatt ctttgtgcaa atgaaagtta tccttcaatc gtgaaacgcg ggggcggtgt
     8641 tcaagatatc tctactcggg aattcatggg gagtttccat gcatacttat ctattgattt
     8701 tttagtggat gtaaaagatg caatgggcgc taacatgatc aactcgattc ttgagagtgt
     8761 ggcaaacaag ttacgggagt ggttccctga agaagagatt ctattcagta ttttaagtaa
     8821 ttttgctaca gaatcattgg cttctgcctg ttgtgaaatc ccattcgaac gcttagggag
     8881 aaacaaagaa atcggtgaac aaattgccaa aaaaatccaa caggcagggg aatatgcgaa
     8941 gcttgaccct taccgtgcag caacgcacaa caaagggatc atgaatggta tagaagctgt
     9001 agtggcagct acaggaaatg atacacgtgc ggttagcgct tcgatccacg cctacgcggc
     9061 aagaaatgga ttataccaag gattaacaga ctggcagatc aaaggggata aactagttgg
     9121 aaaactaacg gtgcctttgg ctgttgccac agtaggcgga gcttccaata tcctgccaaa
     9181 agcaaaagca tctttagcta tgttagatat tgattcagcc aaggaactag cacaagtaat
     9241 tgctgcagta gggttagctc aaaacctggc tgctttgcgt gcattagtta cagaaggaat
     9301 ccaaaaaggc cacatgggac ttcaagctcg ttcattagcg atctcaattg gggctattgg
     9361 tgaggaaatc gagcaagttg cgaaaaaatt acgtgaagca gaaaagatga accagcagac
     9421 agctatacaa atattggaaa aaatccggga aaaataaaaa agcaatatct ttagaagaat
     9481 a
//
LOCUS       DAZKLD010000115         9449 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.115, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000115 DAZKLD010000000
VERSION     DAZKLD010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9449)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9449)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9449
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.115"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            168..362
                     /locus_tag="ACG8KR_002274"
     CDS             168..362
                     /locus_tag="ACG8KR_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0201603.1"
                     /translation="MYYVEVKTKGVKNKQYVKGISNEYPLLGSWKEAAPFSKPCAIKI
                     KNELEKELTCGKAVVDIIEK"
     gene            631..930
                     /locus_tag="ACG8KR_002275"
     CDS             631..930
                     /locus_tag="ACG8KR_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0201604.1"
                     /translation="MDYTVEFDSELLKQFPQEEHFMYKEAISHKVTEYLEHNFFRIKP
                     VRTRSAKGIYEMKVKINKKNYRIAFALKKNHVFIFYISSNLQKIVFDKEVSKKIK"
     gene            complement(1027..1461)
                     /locus_tag="ACG8KR_002276"
     CDS             complement(1027..1461)
                     /locus_tag="ACG8KR_002276"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0201605.1"
                     /translation="MNIDSLMIDEISELYKSVGWTNYTKDTARLARAFEQSESLIKRN
                     GEGKIIGIVRWITDFATIAFIQDILIHPRYQRQGIGKVLLNEALEKITSYGPVQIELL
                     TDDTEKTKKFYESVGFVQVKEMGGISYVIDTRRKKVLFGNDK"
     gene            complement(1693..2403)
                     /locus_tag="ACG8KR_002277"
     CDS             complement(1693..2403)
                     /locus_tag="ACG8KR_002277"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019569 - L-arabinose catabolic process to
                     xylulose 5-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFQ0201606.1"
                     /translation="MSCEEKIAAMKQRVLEANLALPRYGLVKLTWGNVSEVDRELGVI
                     VIKPSGVRYECMQADQMVVTDLSGNIIEEDSLKPSSDLPTHVVLYQTFEDITAITHTH
                     STHSVMWAQAGRDLPAYGTTHADAFYGKVPCTRQLTKEEVREAYEVHTGNVIVETFKE
                     RKLDPNEVPGVLVYGHGPFTWGDSPMKAVENSLILDEICLMAKENELINPNICEIPQY
                     LLDKHYFRKHGKYAYYGQ"
     gene            complement(2393..3253)
                     /locus_tag="ACG8KR_002278"
     CDS             complement(2393..3253)
                     /locus_tag="ACG8KR_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HFQ0201607.1"
                     /translation="MAQIGIYEKALPKDISWKERFSLVKEMDFDFIEMSIDETDERLA
                     RLDWSEEKIAELREEMFSSGVRIHSICLSAHRRFPFGSADPEKKKAAKQLMKKAIHLA
                     HNLSVKVIQIAGYDVYYEEKSMNSREDFIEGIKESVKEASKYGIILSVEIMDDPFMNS
                     ISKFLEIKEQIHSPFLQVYPDLGNLSAWPENNPARELEKGIDYITAIHLKDTYPVTKE
                     YSGQFRDVPFGSGCVDFLGLLRHLKRLDYDGTFLIEMWSEKSQDFRREIQQAKDYLSS
                     KLKEAGYDVL"
     gene            complement(3258..3896)
                     /locus_tag="ACG8KR_002279"
     CDS             complement(3258..3896)
                     /locus_tag="ACG8KR_002279"
                     /EC_number="4.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HFQ0201608.1"
                     /translation="MSRPNLQIALDHNDLEHALGDVMKVGDVVDIIEVGTILCLQEGQ
                     KAIRCIRSMFPDKKLVADTKCADAGGTVASNVAKAGADWMTVICCATIPTMEAAQKEI
                     GELQVELYGNWTFEQAMDWHNIGIRQVIYHQSRDALLSGETWGEKDLSKIKKLIELGF
                     NVSVTGGLNPHTLHLFEGIDVYTFITGRGITAANDPMKAAQNFKDEIIRIWG"
     gene            complement(3911..4210)
                     /locus_tag="ACG8KR_002280"
     CDS             complement(3911..4210)
                     /locus_tag="ACG8KR_002280"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFQ0201609.1"
                     /translation="MRILVSCANGSGTSLMMMRSVEKAMKELNVPITKIHHCAISEGK
                     SSAGQYDVVFTPVNFLNMFTQAEKKGVTVIGIKNVMSVKEISEKFAETKWYQKNK"
     gene            complement(4253..5737)
                     /locus_tag="ACG8KR_002281"
     CDS             complement(4253..5737)
                     /locus_tag="ACG8KR_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFQ0201610.1"
                     /translation="MGEFLLSIWTYFATNILTQPAYLIGFIVLLGYILLKRPLHECIA
                     GFLKATVGYFILTVGSGGLVNNFRPILVGLKERFNLDAMVTDPYFGQNAVDAGLMETF
                     GRTFGDVMILLLIAFVLNILLVRFQKYTKLRSVFTTGNVQVQQAATAFWILLFCFPDL
                     GRIQVLIFMGLILGCYWAVASNLTVGITQELTEGAGFAVAHQQMFGIFIFAKLAEWMK
                     KRDEKKNRSIKQDKKIEDIKLPGFLSIFNENMVATSLLMLFFFGIILIVLGKDYLIQA
                     QFMQEGQSFLFYIMTTSLNFAVYLAILQLGVRTFVDELTQSFQGISNTILPGAVPGID
                     VAATFGFGSPNAVTIGFLFGALGQFLMIGLLILLKSPTIVIAGFIPLFFDNAVIAVYA
                     NNRGGFKAACLFPFLSGIIQVGGSALFATWIGMSTYGGYIGMFDWATAWPLFTVIMKV
                     LGYAGIAVVVIGLLAIPQLQYRKNPDGYFLIVEDYDTYAEKQRV"
     gene            complement(5757..6218)
                     /locus_tag="ACG8KR_002282"
     CDS             complement(5757..6218)
                     /locus_tag="ACG8KR_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFQ0201611.1"
                     /translation="MLKYFFDHQLIHYPDKEITDWREAIAESCLVLIDKEIIDETYVE
                     EIIHCVEKYGPYIVIAPQVAMPHSSEKSDGVFGTAISFTKLKQPIRFESEGENEEKTA
                     SLFFTLAAKDPEEHLENIQKLSELLMTDGLVERLISTNSIDDYQVVMAEYN"
     gene            complement(6236..7303)
                     /gene="ulaG"
                     /locus_tag="ACG8KR_002283"
     CDS             complement(6236..7303)
                     /gene="ulaG"
                     /locus_tag="ACG8KR_002283"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035460 - L-ascorbate 6-phosphate
                     lactonase activity [Evidence IEA];
                     GO_process: GO:0019854 - L-ascorbic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HFQ0201612.1"
                     /translation="MSKTVNEVTKEKWLVETFPEWGTYLNEEIETEVVDPGTFAMWWL
                     GCTGIWLKSHEGTNILCDLWCGTGKRSHGNGMMKKGHQMMRMSGVQNMQPNLRTQPFV
                     IDPFAIKNVDALIVTHIHSDHLDIHTAAAVHQNCPEATFIGPKEVVRTWQKWGIPEEK
                     TITVKPGDAVKIKDINVVALEAFDRTALVTCEDPEEVLKGKMPQEMDQIAVNYLFETS
                     GGNLYHAGDSHYSNLFAKHGNEHKIDVCLGAYGENPRGITDKVTSVDMLRMAESLNAK
                     VVIPVHYDIWSNFMADPKEITEIWKFKKDRLKYGFKPYIWQVGGKFIYPTNKDDLEFN
                     FYRGFEDVFSIENDTPFPSFL"
     gene            7521..8291
                     /locus_tag="ACG8KR_002284"
     CDS             7521..8291
                     /locus_tag="ACG8KR_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFQ0201613.1"
                     /translation="MKNSLVNIEKRQKEILELLQQTEQLSTFELAESLDVSISTIRRD
                     LNLLEEKNEIIRNYGYCSYNYKNQTDFDQSGPERIKQAIAREASKYINDYDTLFINSS
                     STALKTLDFLQSNHLTVITNNVKINYSPHKDNCSYILTGGEMRFPKEALVGDIALNTI
                     SSVNADICIIGCSGVDVVHGVTTKILNESTINEMMVNKTIKCKILVADHRKIGLTSKF
                     KIADISVFDYLITDKFCSEKVLEEIRNTGIKTIQVDSF"
     gene            complement(8564..9391)
                     /locus_tag="ACG8KR_002285"
     CDS             complement(8564..9391)
                     /locus_tag="ACG8KR_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0201614.1"
                     /translation="MIKVDGALLKEYRKKKGLTQVQLAEGICEQATVSNIENKNMSPG
                     INILSALCKKLNVEISDILVETEEDKIRKKINEIQSLNSVSKYQESYKILQTIDQEKV
                     SNSDLLNQILFYQGLLDHLYAKKPDAALFYFNQVLEQTTETDIHHLQAAANVAMIYLS
                     KGELDFAKVYVERTLKILSETDFDNLMVCIVYYDIATYYRKIEDYDKAIQLCEKGIEY
                     NKKHKSTYALEYLLYEIASCHKQLGEDDYLERYMDAKKIARFNGNDYAVKVIENDLK"
BASE COUNT         2940 a         1934 c         1481 g         3094 t
ORIGIN      
        1 taagaacaat agccttttat tatatatatt attcaaatta gttatatcga gtgaaaaaaa
       61 ctttcaaatt gattcttttt atttatcata aagatataga tacataatca ttttcactta
      121 catatatttg aatattaact aaaaacagca agaaagaaga ggatcaaatg tattatgtag
      181 aagtaaaaac aaaaggcgtc aaaaacaaac aatatgtgaa aggtatcagt aacgaatatc
      241 ctttacttgg ttcatggaaa gaagctgctc ctttttccaa accatgtgcc ataaaaataa
      301 agaacgagct agaaaaagaa ctgacttgtg gtaaagctgt tgtagatatc atcgaaaagt
      361 aaatccaatg tatagaaagg aagatcacac atgaaaagcg acgtacaaat tgctaagatt
      421 atagaattag aagaagaaga cgcagtgttg acaattgaaa aaaaataaat gtttgtatac
      481 catatgatta tttcaatatg tatcctggaa ttggagatct cgtagagcta tatcaagatg
      541 aatcaaatat acttgtcatt catcatgaaa tatagagact tataaaattg tttattttta
      601 taatgtatca gtatagaaag gaaataaaac atggattata ctgttgaatt tgacagtgaa
      661 ctacttaagc aatttccaca agaagaacat ttcatgtata aagaagcaat ttcccacaaa
      721 gtgacagagt atttggagca taattttttt cgtataaagc cagtgcgtac tcgttcggca
      781 aaaggaatat acgagatgaa agtcaaaatt aataaaaaaa attatcgcat agcatttgct
      841 ttaaaaaaaa atcatgtgtt cattttttat attagttcga atttgcaaaa aattgtattt
      901 gataaagagg tgagtaaaaa aataaaatag ctgagcattg gaggatagat tgagtgatac
      961 taagtctatc ctcttgtttt tctaaagaac taagctagaa ttttttcttt tgtacgttat
     1021 tatatgttat ttgtcattac caaaaagcac cttttttctt ctagtatcta tgacataaga
     1081 aataccaccc atttccttta cttgaacaaa tcctactgat tcataaaatt tttttgtttt
     1141 ctcagtatca tctgtcaata attcgatttg gactggtcca taactagtga tcttctccaa
     1201 agcttcattc aaaagcactt tgcctattcc ttggcgctga tacctcggat gaatgaggat
     1261 atcttgaata aatgcgatcg tcgcaaaatc agtgatccaa cgaacgattc cgataatctt
     1321 cccttcccca ttacgcttta tcagagattc agattgttca aatgctctag ctaaccttgc
     1381 agtgtctttt gtgtaattcg tccaaccaac gctcttatat aattcagaaa tctcatcgat
     1441 cataagggaa tcaatattca tttttttctt cctttcttat acaattctcc ctctaataat
     1501 acagttcttt gactatttaa tctatcacat ataactctcc tgtaaagatt ctctcgattc
     1561 cttaaaattc ctctttcctt ttagaatgca tactttttga taaataaaaa ggttgtgata
     1621 aattttcttc acaacctctg tttctctcta tcaagtgtta tcttttaatt ttctaccctt
     1681 accctagcat ctctattgcc cgtagtatgc atactttcca tgtttgcgaa aataatgttt
     1741 atctagtaaa tattgcggga tttcacatat attggggttg atcagctcgt tttctttggc
     1801 catcagacaa atttcatcta agatcaaact gttttctact gctttcatag gtgaatcccc
     1861 ccatgtgaac ggtccatgtc catagacaag tactccaggg acttcgtttg gatctaattt
     1921 tctttcctta aacgtctcga caatcacatt ccctgtatga acttcgtaag cttctcttac
     1981 ttcttctttc gttaattggc gtgtacaagg aaccttacca taaaaagcat cagcatgagt
     2041 cgtgccataa gctggcaaat cgcgtcctgc ttgcgcccac atgacggagt gcgtggaatg
     2101 agtatgagta atagcagtta tatcttcaaa agtttgatac aaaacaacat gtgtggggag
     2161 atctgaagat ggttttaagc tatcttcttc tattatattt cccgacaaat ctgttaccac
     2221 catttgatct gcttgcatgc attcatatct gacaccgctt ggcttaatga cgatgactcc
     2281 tagctcacgg tccacttcac tcacattgcc ccaagtcaat ttgactaatc cgtatcgcgg
     2341 caatgccaga tttgcttcaa ggacacgttg cttcattgct gcaatttttt cttcacaaga
     2401 catcatagcc agcctccttc aatttagaac ttaagtagtc ttttgcctgc tggatttctc
     2461 ttcgaaagtc ttggcttttt tcactccaca tttcaatcag aaatgttcca tcataatcta
     2521 gacgtttcaa atgtcttaat aatccaagaa aatcaacaca accgctacca aaaggaacat
     2581 cacgaaactg gcctgaatat tcttttgtta cagggtaagt atcttttaag tggatagcag
     2641 tgatataatc gatccctttc tcaagttctc gagccggatt attttccggc catgcggaca
     2701 aattgcctag gtctggatat acttgtagaa aaggtgaatg aatctgttct ttgatttcta
     2761 aaaattttga aatggagttc ataaatggat catccatgat ttccacggaa agaatgatgc
     2821 catatttaga agcttccttt acgctttctt ttattccttc gataaagtct tctctggaat
     2881 tcattgattt ctcttcataa taaacatcgt atccagctat ttggattact ttgacgctta
     2941 gattatgcgc caaatggatt gcttttttca ttagttgttt cgctgctttt ttcttttctg
     3001 gatcagcaga accaaacgga aatcgccgat gcgcacttaa acaaatcgaa tggatacgta
     3061 caccggagct gaacatctct tctctcagct cagctatctt ttcttcagac caatctaatc
     3121 gtgctaaccg ctcatccgtc tcatcaatcg acatctcgat aaaatcgaag tccatttctt
     3181 ttacaaggga aaaacgttct ttccatgaaa tatctttggg taacgctttt tcataaatcc
     3241 caatttgagc catttcctta cccccagata cgaatgatct catctttgaa gttttgtgcg
     3301 gccttcatcg gatcatttgc tgctgtaata ccacgaccag tgataaaggt atacacatcg
     3361 atcccttcaa ataagtgtaa tgtgtgtgga tttaatccac cagttacaga aacattaaag
     3421 cctagttcaa tcagtttttt gatcttcgat aaatcttttt caccccaagt ttcaccagat
     3481 aacagcgcat cacggctttg atggtagatt acctgccgga taccgatgtt atgccaatcc
     3541 atggcttgtt caaaagtcca attgccgtag agttctactt gcaattcacc tatttctttt
     3601 tgtgctgcct ccatagttgg aatcgttgca caacaaatga ctgtcatcca gtctgctcct
     3661 gcttttgcaa cattactcgc cactgttcct ccagcgtctg cacatttcgt atcagcgact
     3721 agttttttat ctgggaacat actgcgaata cagcgaatcg ctttttgtcc ttcctgtaag
     3781 caaagaattg tgcccacttc aataatatct actacatcac caactttcat aacatctcct
     3841 aatgcatgtt ctaagtcatt gtgatctaac gcaatttgca aattcggcct gctcatctta
     3901 gttccttcct ttacttgttt ttttggtacc actttgtttc agcaaatttt tcactgattt
     3961 ctttgactga catcacattt ttgataccga taactgtaac tccttttttc tctgcctgag
     4021 taaacatatt caaaaaattg actggcgtaa aaactacatc gtattggcca gctgaacttt
     4081 ttccctctga aatggcacaa tgatggattt tggtaattgg tacgtttagt tctttcattg
     4141 ctttttccac acttctcatc atcatcaagc ttgttcctga tccatttgca cacgatacta
     4201 aaattctcat cttaatccct ccattatgag tgttgtctaa tcagattttc ttttatactc
     4261 tctgcttttc tgcatatgtg tcatagtctt ctacaatcaa aaaataacca tcaggatttt
     4321 tccgatattg taactgcgga atagccaata aaccaatgac aactacggca attccagcat
     4381 aacctaggac tttcatgatt actgtaaaca atggccaagc tgttgcccag tcgaacatcc
     4441 cgatataacc accgtatgtt gacatcccaa tccatgtagc gaataatgct gaaccgccta
     4501 cttgtatgat tcctgaaaga aacgggaaca ggcaagcggc tttgaatcca cctcggttat
     4561 tggcataaac tgctataacg gcattatcaa agaacaacgg aatgaaaccg gctatgacga
     4621 tcgttggtga tttcaataaa atcagtaaac caatcatcaa aaactgtccc aaagcaccaa
     4681 acaagaaccc aatcgttacc gcatttggcg aaccaaagcc aaaagtagca gctacgtcaa
     4741 ttcctggaac agctcctggt aaaatcgtgt ttgagattcc ttggaaagat tgagttagtt
     4801 catcgacgaa tgtccgaaca cctaattgca agatcgccag atataccgca aaatttaaag
     4861 aagtcgtcat aatgtagaat aagaaactct gcccttcttg catgaattgc gcttgaatca
     4921 aataatcttt gcctagtaca atcagaatga tcccaaagaa aaatagcatg agtaacgaag
     4981 tagcaaccat attctcattg aaaatcgaca agaatcctgg taatttgata tcttcaattt
     5041 ttttgtcttg cttgattgat ctatttttct tttcatcccg ctttttcatc cactcagcta
     5101 attttgcaaa tataaagatt ccgaacattt gctgatgtgc cactgcaaat ccggcacctt
     5161 ctgtcaattc ctgcgtgatt cctactgtta agttagaagc tactgcccaa taacaaccta
     5221 aaatcagtcc cataaaaatc aatacttgta tcctacctag atcaggaaag caaaacagta
     5281 aaatccaaaa agcggtagct gcttgttgaa cctgaacgtt tccggtagtg aaaacggaac
     5341 gcagtttcgt atatttctga aaacggacta ataagatatt taaaacaaat gcaatcagta
     5401 ataaaatcat cacatctcca aaggtacggc caaatgtctc cattaatcca gcatccacag
     5461 cattttgtcc aaaataagga tctgtaacca tggcatccaa attgaaccgt tcctttagtc
     5521 caaccaagat tggccgaaaa ttattgacca aaccaccaga gccaacagtc aaaataaagt
     5581 aaccgaccgt tgcctttaag aatccagcga tgcattcatg aagcgggcgt ttcagcaaaa
     5641 tatagcctaa taacacgata aaaccaatca ggtatgctgg ctgcgtcaag atatttgttg
     5701 caaaatacgt ccaaatgctt aacaaaaatt cccccatctt ttttctcctc tatcaattaa
     5761 ttatattctg ccatcacaac ttggtaatca tcgatggagt tcgtagagat gagacgctca
     5821 accaatccat ctgtcattaa taattcggat aatttttgga tattttctag atgttcttct
     5881 ggatcttttg ctgcgagtgt aaaaaacaaa ctagccgttt tctcctcatt ctccccttca
     5941 ctttcaaaac ggattggctg tttcaacttg gtaaatgaaa tagctgttcc aaaaacacca
     6001 tcactttttt ctgaagaatg cggcattgct acttgaggtg cgatcacaat ataaggaccg
     6061 tatttttcta cacaatggat gatctcctcc acatacgttt catcaataat ttccttatcg
     6121 atcaatacaa gacagctttc ggcgatagct tcacgccaat ccgtgatttc tttatctggg
     6181 taatgaatca attgatggtc aaaaaagtat ttcagcaaga aactcccctt ctttcttata
     6241 aaaacgacgg aaacggtgta tcattttcaa tcgagaagac atcttcaaat cctcgataaa
     6301 aattaaactc aagatcatct ttgtttgtcg gatagatgaa tttcccgcct acttgccaga
     6361 tataaggttt aaatccatac ttcaggcgat cctttttgaa tttccaaatt tcagtgatct
     6421 cttttggatc agccataaaa ttagaccaaa tatcgtagtg gacaggaatc actacttttg
     6481 cattcaatga ctccgccatg cgcagcatat caacagaagt aactttgtct gttatccctc
     6541 gtgggttttc accataggcc cctaaacaga catcgatttt gtgttcatta ccatgtttgg
     6601 caaataaatt ggagtaatgt gaatctcctg catgataaag attccctcct gatgtttcga
     6661 ataaatagtt tactgcaatt tgatccatct cctgaggcat ttttcctttt aatacctctt
     6721 ctggatcttc acaagtgacc aacgctgtcc gatcaaacgc ttctaaagct actacattga
     6781 tgtctttgat tttgactgca tcacctggct ttactgtaat cgttttttct tcaggaattc
     6841 cccatttttg ccacgtgcga accacttctt ttggcccaat gaatgttgct tcaggacaat
     6901 tttgatgaac tgctgcggct gtatggatat ctagatgatc ggaatggata tgtgtaacga
     6961 ttaaagcgtc tacatttttt atcgcaaaag gatcgattac gaaaggctga gtacgtaaat
     7021 taggctgcat attttgaacg ccactcatac gcatcatctg atggcctttt ttcatcatgc
     7081 cattcccatg actacgtttt cccgtcccac accatagatc acataaaata ttcgttcctt
     7141 catgactttt cagccaaatt cccgtacagc ccagccacca catagcaaaa gttccaggat
     7201 ccactacttc tgtttcgatt tcttcattca agtatgtgcc ccactcagga aatgtttcta
     7261 ctaaccactt ttcttttgtt acttcattga cagttttact catttgcttg ctccccccat
     7321 atcgttgttc caacttacaa gtaaatatta atgtgtatca gcatccatga aaaccctttt
     7381 atatgaccgt ttgtttttta tatgattttc tttcagtaat caatgattgt ttcagtataa
     7441 taaagacaga tatgaatgtt taaatgaaca actagtcaaa taaatccatg gatcaagatg
     7501 tccctcagga ggcaaaagtc atgaaaaact cacttgtcaa tattgaaaaa cgtcaaaaag
     7561 aaattttgga actgcttcag cagactgaac aactatcaac tttcgaatta gctgaatccc
     7621 ttgatgtatc tattagtacg atacgaagag atttaaattt attagaagaa aaaaacgaga
     7681 tcatacgcaa ttacggttat tgttcttata attacaaaaa ccagactgat tttgatcaat
     7741 ctggtcctga aaggattaaa caagcgattg ccagagaagc tagtaaatac attaacgatt
     7801 atgatacgct gtttatcaat tctagctcga ctgcgttgaa aacactggat tttcttcaat
     7861 ctaaccattt gacagtcatt acaaacaacg tgaaaatcaa ttactctccg cacaaagata
     7921 attgtagtta tattttaact ggtggagaga tgcgctttcc aaaagaagca ctagtgggcg
     7981 acatcgcatt aaatacgatt tcttcagtca atgcagacat ttgtatcatt ggctgtagcg
     8041 gagtcgatgt agtacatggg gtcacaacaa agatattaaa tgaatcaacg atcaacgaaa
     8101 tgatggtcaa caagacaatc aaatgtaaga ttttagtcgc tgatcatcga aaaatcggac
     8161 tgacatcaaa gttcaagatt gctgatattt cagtctttga ttatttgatc actgataaat
     8221 tttgttctga aaaagtatta gaggagatca gaaatacggg gataaaaacc attcaagtag
     8281 attcatttta agaaccagga cgtaaatatg taaataggat tatcaaaaaa atgaataaca
     8341 aatttatcga taaatgaaaa acacgctacg ctcaaattga taaaggaatc ccaagatgta
     8401 tccatccaat cgataccatc ataatctatt gcttctttta taaccgttat atgttgccta
     8461 aataggaatc aaacaatatc gaaaaaagga ggtatgacaa aagtcacacc tccttttttc
     8521 gaataaacgg tattcaaatc agaacactga tttcacaacc actttatttc aggtcgtttt
     8581 caatcacctt taccgcgtaa tcatttccgt tgaaacgcgc tatttttttt gcgtccatat
     8641 atctttctaa ataatcatct tctcctaatt gcttatggca agaagcaatt tcataaagca
     8701 agtattccaa tgcataagtc gatttatgtt tcttattata ctcaataccc ttctcacata
     8761 attggattgc tttatcataa tcctcgattt tacggtaata ggtggcgata tcataatata
     8821 caatgcagac catcagatta tcaaaatcag tctcactcaa tattttaagt gtacgctcca
     8881 cataaacttt cgcaaaatct aattctcctt ttgacaagta gatcattgcc acgtttgcag
     8941 ctgcttgtaa gtgatggata tctgtttcgg tcgtttgttc caacacttga ttaaaataaa
     9001 ataatgctgc atctggtttt ttcgcataaa gatgatcaag cagtccttga taaaataaaa
     9061 tttgatttaa aagatcagaa ttacttactt tttcctgatc gatcgtttgt aaaattttat
     9121 aagattcttg gtattttgat acagaattca atgattgaat ctcatttatt tttttcctga
     9181 ttttgtcttc ttctgtttct actaatatat cggagatctc gacattcagc tttttgcaaa
     9241 gcgcacttaa gatattgatg cctggcgaca tattcttgtt ttcgatattg ctgacagttg
     9301 cttgttcaca aatgccttct gctaattgta cttgtgttaa cccctttttc tttcggtatt
     9361 cttttagtaa tgctccatct actttaatca aaacaatttc cttcccttta aaaaattatt
     9421 tctcctttat atcatacaca aaaaaaagt
//
LOCUS       DAZKLD010000116         9444 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.116, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000116 DAZKLD010000000
VERSION     DAZKLD010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9444
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.116"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            59..205
                     /locus_tag="ACG8KR_002286"
                     /pseudo
     CDS             59..205
                     /locus_tag="ACG8KR_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305108.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
     gene            282..611
                     /locus_tag="ACG8KR_002287"
     CDS             282..611
                     /locus_tag="ACG8KR_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFQ0201615.1"
                     /translation="MITKTRKQGNSIMLTVPKDFNVPNGVEVEAKLVENGILYEFVEP
                     QKEFYDFSEDILSDIIAEGYDKDEILVEFKNRKNKMHSSFRDIAEDTLTNSKVMTKEE
                     LATEIGL"
     gene            601..888
                     /locus_tag="ACG8KR_002288"
     CDS             601..888
                     /locus_tag="ACG8KR_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFQ0201616.1"
                     /translation="MAYEIVPSKHVIKYLKKLKEKPLKEKFLNLIYDEIAIDPYKGEQ
                     KTGDLSGIWSSGFKYAGTTYRVAYEIIENKVIPVLLCGTHENFYEQLKKLI"
     gene            1174..2031
                     /locus_tag="ACG8KR_002289"
     CDS             1174..2031
                     /locus_tag="ACG8KR_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HFQ0201617.1"
                     /translation="MVAKKYVIGNFKGGVGKSTCAQMLGFESAVNEGKRTLIIDLDMQ
                     GNTSDVMNLTHMNFTNEEGGGEGEPIVYENTVTDVLINNLPPKEAVYKVINNLYILPA
                     DMSFEMYDDWIKDQYNDSLSQFKYMKEKLEPLFDEFDVIYLDVPPSISVYSKSAMYIA
                     DWAIVVLQTQVKSMRNAMQYLEYMDFFVKEFDTNLYVAGVIPFMLESGDAVDQEMYKQ
                     AQEIYGEHLIKNVVLKNARLKRYDGSGITMEKTKKGLLKQWDRKSHELFISILKELEE
                     HENWYVEVE"
     gene            2032..2580
                     /locus_tag="ACG8KR_002290"
     CDS             2032..2580
                     /locus_tag="ACG8KR_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0201618.1"
                     /translation="MADIKGLDIFGNNKGLAKIKKEAEAKKKQQAITPSENKQLEVHQ
                     VQEIKTSSNVERQSRGRTKKVGAPIRKFDKVYSTKHPLKLSTLLNATSRVLVEKYETN
                     MTRDELLRKALDEYIKQNLTQEDKQDLFNDVVKELDLFREKYPIVPELDDRGEIIRTT
                     SQIKQDTEDSLRKGWGMNNKVR"
     gene            2840..3301
                     /locus_tag="ACG8KR_002291"
     CDS             2840..3301
                     /locus_tag="ACG8KR_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermophilic serine proteinase"
                     /protein_id="HFQ0201619.1"
                     /translation="MKKFKKKIYLGLVVGVSMFGFMAHTKQVTADTNQKERIMSQLDD
                     SQIEYVPNELIVSLKATQRISTEKNKPQNLLGVKVMVEKDLTLRTNESDYNKRDQVLL
                     VKLNEEISVEEAVRTFNASDMVNYAEPNYVVELEPLNDVEEPNTTDSLGNR"
     gene            3345..3431
                     /locus_tag="ACG8KR_002292"
                     /pseudo
     CDS             3345..3431
                     /locus_tag="ACG8KR_002292"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
     gene            complement(3397..4467)
                     /locus_tag="ACG8KR_002293"
     CDS             complement(3397..4467)
                     /locus_tag="ACG8KR_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFQ0201620.1"
                     /translation="MKNDIVTYANTKCEEIGISNLEECFFDTNDLSQFLRTGRTNASK
                     LANDLVAKNVLFKINTRPVLFAIKERFEEHFLIPVKDVYESISELKTDLGPNSTDSIL
                     QKMIGYDMSLSDAVEQMKTAVFYPGRGLPMMITGDTGVGKSFFVHTAYQFMKKVNVIH
                     ENAPFKVLNCAQYHNNPELLSGILFGYVKGAFTGADEDRNGLLQDADGGVLFLDEVHR
                     LPPEGQEKLFVYMDGGTYSRVGETIHHASNVRLVFATTEGKEDFLETFMRRIPIHIHI
                     PNIEERGILEKNKLSKILSLAKAKTLARLLMFLLKSLVLHKYSQNILNYYFLEKSKKN
                     RIPNFHLHFQKDLHVDNQFLNQ"
     gene            5069..6913
                     /locus_tag="ACG8KR_002294"
     CDS             5069..6913
                     /locus_tag="ACG8KR_002294"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFQ0201621.1"
                     /translation="MNYKQTAEEVLQYIGGEENISHLEHCSTRLRFTLKDNSKVNMSQ
                     LEKVDGVMGVRQNVQCQVIIGNDVVEVYDEVKALLGDNVSNTEEAPKQKQKLGSVLLD
                     FIISIFQPLIPAIAGGGVLKSLLLLASILGLISDQSQTYQILNLVGGAPLYFLPILVA
                     MTTANKLKVNQLVAVSAVGALILPEMTAMLAEGATFLSFGLDNIAYASQVFPAILTVL
                     FYAQLEKWFTKVSPKPIRIFFVPMMSLLITVPIALLLLGPLGYNVGTAFSSIIVSLYN
                     QFGWVATGVLAAVLPLMVVTGMHKAMIPYAVSSMSELGAELLYLPASLAHNISEAGAC
                     FAVSIKTKDKKLKSTAVSAGISALFGITEPALYGVTILHKRVLYAVMFSSLIGGAFAG
                     IVALQAFALVGPGIASITMFADKADPMNLVWAFAVSGISFVVAFAAALVLYKDKEVVN
                     EVETKMETAVVSDAVLSSPVNGKVISLSEVKDTVFSSGMVGDGVAIIPEEGILVAPED
                     GEITMVFETKHAIGLKTANGAELLFHIGIDTVQLEGKGFEAFVRSGEKVAKGQKLIAF
                     DIDLIKEAGFDPTVICVVTNKDNYTIRSFNLEKLVTNDSDLLAISPIA"
     gene            6969..8435
                     /locus_tag="ACG8KR_002295"
     CDS             6969..8435
                     /locus_tag="ACG8KR_002295"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016620473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFQ0201622.1"
                     /translation="MTNTTSFPKGFLWGGAIAANQVEGAWNVDGKGLSVADMATYKPN
                     LDNKDFAAHMHVTLQSIKEASEDKSDKWYPKRRGVDFYHHYKEDLALFAEMGFKTLRL
                     SIAWTRLFPTGEELEPNEKGVAFYENVFKEMQRLGIEPIVTLSHYEMPLALAVKYNGW
                     VERRVIDDFVRFANVCFDRFGKYVKYWLTFNEIDSIHRHPFTTAGIIQEKSAEGQEAQ
                     DIYQALHHQFVASALVTKDAHEKIPGSQVGCMLTKLMTYPLTCAPEDVELTLKKNLEN
                     NFYADVQVKGEYPALIEKDLENRGIQIKMEDEDREILKKHTVDFVSFSYYMSMAESAD
                     PNAERTPGNTVLGVKNPHLPSTDWGWQIDPKGLKISLIELYDRYQVPLMIVENGMGAV
                     DQVVDGKIHDPYRIDYFKAHFQQMKEAIDEGVDLIAYTSWGPIDLVSAGTSQMSKRYG
                     FIYVDADDEGNGTYNRLKKDSFNWYKEVIETNGASLFE"
     gene            8472..9314
                     /locus_tag="ACG8KR_002296"
     CDS             8472..9314
                     /locus_tag="ACG8KR_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFQ0201623.1"
                     /translation="MISIKKVLNSSVVLVEENGKEMIALGKGIGFGQKAGDEISGDKV
                     DKIFLPFEEKKSSQLLELVEEIPIEFFEITRTIVFEAEKGLDKKLNPGVYLTLTDHLH
                     FAVERAQKGVNIANRLYWEIKIYYPKEFQIGKYALTLLNEQYGIDLPKEEASNIAFHL
                     INAQSEDPDSGAGFKYAKMIGGIVNLVRYSLQKDIDTNSIHYARFITHVRFFVERYYS
                     DALLDDGEEEFYNQMWILYPSAMESAAKIKDYIEKVYHKTIPNDEVVYLGVHINRLMK
                     HANG"
BASE COUNT         3246 a         1444 c         1906 g         2848 t
ORIGIN      
        1 agcttaaatc atagataccg taagggattt tattctttat ttaaaacttt gcaacagaac
       61 ccaatcattt gagaagttaa aacctacaca tgaaggtagg tattttagaa gattgaatag
      121 acaacaccgg gttgtttata aggtagatga agatgaacac gtggttgaaa tttattcagc
      181 aaggacgcat tacgagtcac agtaattact tgtaaataca ttatctcgtg gtataatgta
      241 tttactaagt aattacatac tgttagaaat gagggattta aatgattaca aaaacaagaa
      301 aacaaggcaa ttcaatcatg ttgacagtac ctaaagattt taatgttcct aatggagtcg
      361 aagttgaagc aaagttggta gagaatggta ttctttatga atttgttgaa ccacaaaaag
      421 aattttatga ttttagcgaa gatattttgt ctgatataat tgcagaaggc tacgataaag
      481 atgagattct tgtagaattt aagaatagaa aaaacaaaat gcattcttcc tttagagata
      541 ttgctgaaga tacacttact aattctaaag taatgactaa agaggagttg gcaacagaaa
      601 ttggcttatg aaatcgttcc ttcaaaacat gtcattaagt atttaaaaaa gctaaaagag
      661 aagccattga aagaaaaatt tttgaattta atttatgatg aaattgcgat tgatccatat
      721 aagggtgaac aaaaaactgg ggatttatca ggtatttggt ctagtgggtt caaatatgcg
      781 ggcacaacat atagagtagc ttacgagatt atagaaaaca aggttattcc agtattgtta
      841 tgtggaacgc atgaaaactt ttatgaacag ttgaaaaaat taatctagtg atttgttgtc
      901 gttcttaaga aggacggctt cttttttatc gaaaatctga ttcgtctaca tgactaaagt
      961 cgcgagtatt ctctgcttct cgataaatta aataacgtca ttactttgag gaaagctata
     1021 aatcaatata tttcagcttt tccttattta tgtactacct tttacttatt aattataagg
     1081 tagtacatat atatcttttt tttggtagta actaaaaata aatatgttat aatagtatca
     1141 aatgaatatt gctaaaagaa aagaggtagg aacgtggtag cgaagaaata tgtaatcggt
     1201 aattttaaag gtggcgtagg taaaagtacg tgcgcgcaaa tgttagggtt tgaatcagca
     1261 gttaatgaag gaaaaagaac cttgataatt gacttggata tgcaaggaaa tacttctgat
     1321 gtgatgaact taacgcatat gaacttcact aacgaagaag gaggcggaga aggtgaaccg
     1381 attgtttacg aaaatactgt gactgacgtg ttgataaaca atcttccacc taaagaggct
     1441 gtctataaag taatcaataa cctctatata cttccagcag acatgtcctt tgaaatgtat
     1501 gatgattgga tcaaagacca atataatgat tcattatccc aattcaaata tatgaaagaa
     1561 aagctagaac cattatttga tgagtttgac gtcatctatt tagatgtccc accttctatt
     1621 tctgtatata gtaaatccgc gatgtacatt gcggattggg caatcgtcgt cttacagacc
     1681 caagtaaaat cgatgagaaa tgcgatgcaa tatcttgagt atatggactt ttttgtgaaa
     1741 gaatttgata caaatttata tgttgctggt gttattcctt tcatgttgga aagcggtgac
     1801 gcagtagatc aggaaatgta taaacaagcc caagagattt atggggaaca tttgattaaa
     1861 aatgttgtac tcaaaaatgc gcgcttaaaa cgatacgacg gatcaggtat tacgatggag
     1921 aagacaaaga aaggtctctt aaaacaatgg gatcgcaaaa gccacgaatt atttatcagt
     1981 attttgaaag aattggaaga acacgagaat tggtatgtgg aggtggagta attggctgac
     2041 atcaaaggac ttgatatttt tgggaacaac aaaggactcg ccaaaataaa aaaagaagca
     2101 gaagcaaaga aaaagcagca agcaattaca ccatctgaaa ataagcaatt agaagtgcac
     2161 caagttcaag aaataaaaac ttcctccaat gttgaaagac agagtagagg aagaacaaaa
     2221 aaagttggcg caccaatcag aaagtttgat aaggtttatt ccaccaagca tcctttaaaa
     2281 ctgtcgactt tgttaaatgc gacttccaga gttttagtag aaaagtatga gactaatatg
     2341 acaagagatg aattgcttag aaaagcttta gatgagtata ttaaacagaa tcttacgcaa
     2401 gaagataaac aagatttgtt taatgacgtg gtgaaagaac tagacttgtt cagagaaaaa
     2461 tatccaattg ttccagaatt agatgatcgt ggagaaataa taagaactac cagtcagata
     2521 aaacaagata ccgaagactc actgcgcaaa gggtggggaa tgaataataa agttagataa
     2581 taatcaaaga agaactaagt aactaccttt aaacggtagt tacttagttc ttctttgatt
     2641 tgcgtgaaat agaggggatt tatttttctg gttacgtgat taaaaaatcc aaaaaatgag
     2701 cataggaacg gttatgattt tagggtgtgt tttaatgaga tattgtgatt tcattaataa
     2761 gtgtatgata atctgttgtt gagttagtaa ataagtaata gagctaactc taaaataaaa
     2821 ctattaggag gagatgtaga tgaaaaagtt taaaaagaaa atatatttag gattggttgt
     2881 tggggtgagt atgtttgggt tcatggctca tactaaacaa gtaactgcag atactaacca
     2941 aaaagaaagg attatgagcc aattagatga ctctcaaata gagtatgtac ccaatgagct
     3001 aattgtcagt ttaaaggcta cacaaagaat ttcaacagag aaaaataaac cgcaaaattt
     3061 attgggtgta aaagttatgg tggaaaaaga tttgactctt aggacaaatg aaagcgatta
     3121 taataagcga gatcaagtat tattagtgaa attgaacgaa gaaattagtg tggaagaggc
     3181 agtaagaaca tttaatgctt ctgatatggt caattatgca gagccaaact atgtggttga
     3241 actagagccg ttgaatgatg ttgaggagcc aaatacaact gattctttag gaaatcgata
     3301 acgatcctaa ttggagtaaa caagctaatt taagagagat tagcatgcca tccgcttggg
     3361 aagaaaccaa gggttctaag gatgtgaagg tagcggtcat tgattcagga attgattatc
     3421 aacatgaaga tctttctgga aatgtagatg aaagtttggg atacgatttt ttttactttt
     3481 ttcaagaaaa taataattca gaatattctg actatattta tgcaacacta gtgactttag
     3541 gagaaacatc aatagccttg ctaaagtttt tgctttcgct aaagagagta ttttcgataa
     3601 tttgtttttt tctaaaatac ctcgttcctc aatatttggg atatggatat gtatagggat
     3661 tcttcgcata aaggtttcca gaaaatcctc tttgccttct gtagtcgcaa aaactaaacg
     3721 tacattggag gcgtgatgga tagtttcacc gactctagaa taagttccgc catccatata
     3781 aacaaatagt ttctcttgcc cttcaggagg caatctgtgt acttcgtcta aaaacagtac
     3841 tccaccgtct gcatcttgca acaatccatt tcgatcttca tctgcgccgg taaaagcacc
     3901 tttaacatag ccaaatagta tccccgacaa taattcagga ttattatggt attgcgcaca
     3961 attaagtact ttaaaaggag cattctcatg aataacgttc acttttttca taaactgata
     4021 agcagtatga acaaaaaaac tttttccaac tccagtatct ccagtgatca tcataggaag
     4081 ccctcttcca ggatagaaga cagcagtttt catttgttcc accgcatcgg ataagctcat
     4141 atcataccca atcatctttt gtagaatgga atctgtagaa ttgggcccca agtctgtttt
     4201 taattcagat atactttcgt atacatcttt taccggaatc aaaaagtgct cttcaaagcg
     4261 ctctttgatg gcaaataaca ctggacgagt attgatttta aatagaacgt tctttgcaac
     4321 taagtcattg gcaagtttac tggcatttgt tcttcccgtt cttaaaaatt gcgacaggtc
     4381 atttgtgtca aaaaagcact cttctaaatt actgatgcca atttcttcac attttgtgtt
     4441 tgcataagtc actatatcat ttttcatctt ataaacagtc ctttctaaac ttctatccct
     4501 taaataaata gcaaaaaaaa ggaagcaata tcgatatgct gtcctcttaa tttaataaag
     4561 cgctaatcta attcactgta ttatcatttt ttagtagtat agcacaatta tgaatgcctt
     4621 ttctttatat caaataaagt ttatataaaa aacctcatcc tgcaagaaag aaaaaatgaa
     4681 cccattcttt ttatactagg tcttaaaaag acagaacatc tgctagaaga tagaagagaa
     4741 taagagttat atatgttgga agatattctt agaaaaaaga caaaactttt agaacacgaa
     4801 aactatattg aacgaataaa atgaaaaaat gattaaaaaa tcattttatt atttacgttt
     4861 acattctaat atgttaaatt gaatataact taaacaggca tgtgactggt aatgcaggca
     4921 ggacctaaat aagttgaata acaatgtatg cgtttcaata cacgtttgcc ttgttgtcaa
     4981 tttatttagt ttctgcctgt tttttgtgtt gaggatggcc acttcgactc aaaagttcat
     5041 cactcaaaaa agaaaagagg gaaacaccat gaattacaaa caaacagcag aagaagtatt
     5101 gcagtacatc ggtggggaag aaaacatatc ccatttggag cattgctcaa ctcgtttacg
     5161 ttttacgtta aaagacaaca gcaaggtaaa tatgagccag ttagaaaaag tggatggcgt
     5221 tatgggcgtt cgccaaaatg tgcaatgcca agtaatcatt ggaaacgatg ttgttgaagt
     5281 gtatgatgaa gtgaaagcgt tattaggtga caatgtttca aatactgaag aagcaccaaa
     5341 acaaaaacaa aaattaggtt cagttctatt agattttatt atttctattt tccaaccttt
     5401 aattcctgcg attgcaggtg gaggagtatt gaagtccttg ttgctactag cttctatctt
     5461 agggctgatt agtgaccaat ctcaaaccta tcagatttta aatttggtcg gcggggcacc
     5521 gctttatttc ttgcctattc tggtggcaat gactacggct aataaattga aagtcaatca
     5581 attggttgca gtatctgctg tcggtgcatt gatccttcct gaaatgacag ctatgttggc
     5641 ggaaggtgct acattcttat cttttggatt ggataatata gcgtatgcat ctcaagtatt
     5701 cccagccatc ttaactgttt tattttatgc tcaacttgaa aaatggttta caaaagtttc
     5761 accaaaacca attcgtattt tctttgtacc tatgatgagc ttattgatta cagttcctat
     5821 tgctttgttg cttctaggac cattaggtta taatgtagga acagctttct cttctatcat
     5881 tgtgtctttg tacaatcaat ttggatgggt tgcaaccgga gtcttagcag ctgtactgcc
     5941 attaatggtt gtaacgggta tgcataaagc aatgatccct tacgctgttt catccatgag
     6001 tgaattgggt gcagaacttc tttatctccc ggcttcattg gcacacaata tttcagaagc
     6061 tggcgcatgc tttgctgtca gtatcaaaac aaaagacaaa aaattgaaat ctacggctgt
     6121 ttctgcgggt atatctgcat tattcggaat tactgaacca gctctttacg gggtaacgat
     6181 tctgcacaaa cgtgttttat atgccgttat gttttctagc ctgatcgggg gagcatttgc
     6241 aggaatcgtt gcacttcaag cctttgcttt agtgggtcct gggattgcaa gtatcacgat
     6301 gttcgctgac aaagctgacc caatgaatct ggtttgggca tttgcagtat cggggatttc
     6361 atttgtggtt gcttttgctg cagcgttggt tctttataaa gataaagaag ttgtcaatga
     6421 agttgaaact aagatggaaa cggccgttgt ttcagatgct gttctttcaa gtcccgtgaa
     6481 tggaaaagta atctcactat cagaagtaaa agacactgtt ttttctagtg gtatggtagg
     6541 tgatggagta gcaatcattc ctgaagaagg gatattagta gctcctgaag atggagaaat
     6601 cacaatggta tttgaaacga agcatgctat tggtttaaaa acggctaatg gtgcagaact
     6661 tctttttcat attggtattg atactgttca gttagaagga aaagggtttg aggcatttgt
     6721 tcgttctggt gaaaaagtag ccaaaggaca aaagctgatt gcatttgata ttgatttaat
     6781 caaagaagca ggattcgacc ccacagttat ctgtgtggta accaataagg ataattacac
     6841 tatccgttca tttaacctag aaaaattggt aaccaatgat agtgatctct tagctatcag
     6901 tccgatagct taaacacaag atagtgaaaa aaaatatatt gaatgaagga atagaggaga
     6961 atagaataat gacaaacaca acaagttttc caaaaggatt tttatggggc ggcgcgatag
     7021 cagcaaatca agtagaaggt gcttggaatg tagacggtaa aggtctttca gtagcggata
     7081 tggctactta taaaccaaat ttagacaaca aagattttgc tgctcatatg catgtcaccc
     7141 tgcagtctat aaaagaagct agtgaagaca aatctgataa atggtaccca aaaagaaggg
     7201 gcgttgattt ttatcatcac tataaagaag atttagcttt atttgccgaa atgggcttta
     7261 aaaccttgcg cctctctatt gcttggactc gcttgttccc tacaggagaa gagctggaac
     7321 cgaatgaaaa aggggttgca ttttatgaaa acgtctttaa agaaatgcaa cgtttaggaa
     7381 ttgagccgat tgtgacacta tctcattatg aaatgccatt agctttagca gttaaatata
     7441 atggctgggt ggagcgtcga gtaattgatg attttgttcg cttcgctaac gtttgttttg
     7501 atcgcttcgg caaatacgta aaatattggc tgacatttaa tgagatagac agcatccacc
     7561 gtcatccatt tacaacagct ggaattatcc aagagaaaag cgcagaaggt caagaagctc
     7621 aagatatcta tcaagcattg catcatcaat ttgttgcgag tgcgttagtg acaaaagatg
     7681 ctcatgaaaa aattccagga agccaagttg gttgtatgtt aacgaaattg atgacttatc
     7741 cattaacttg cgcaccagaa gacgttgagc tgaccttgaa aaagaactta gaaaataatt
     7801 tttatgcaga tgttcaagtt aagggagaat atcctgctct aatcgaaaag gatttggaaa
     7861 atcggggaat tcagattaaa atggaagatg aagatcgaga aattttaaag aaacataccg
     7921 tagattttgt ttctttcagc tattacatgt ccatggcaga atcagctgat ccaaacgcag
     7981 aacgcacacc gggaaatact gttttagggg ttaagaatcc tcatttgcct tcaacagatt
     8041 ggggctggca aatcgatcca aaaggtttga aaatttcgtt gatcgaactt tatgatcgtt
     8101 accaagtacc gttgatgatt gtggaaaatg gtatgggagc agtcgatcag gtagtagatg
     8161 gtaaaatcca tgatccgtac cgcatcgact actttaaagc acacttccaa caaatgaaag
     8221 aagcaatcga tgaaggtgta gatttgatag cttatactag ctggggacca attgatttag
     8281 ttagtgcagg aacttctcaa atgtctaaac gatatggatt tatttatgtg gatgcagacg
     8341 atgaaggaaa tgggacatat aaccgcttga aaaaggattc gttcaactgg tacaaagaag
     8401 tgatcgaaac aaacggtgca tccttattcg agtagtctcg atctttttaa gcagcgatag
     8461 ggggataatg agtgatttca ataaaaaaag tattgaattc aagtgtggta cttgttgaag
     8521 aaaatggcaa agaaatgatt gcattaggta aagggatagg ctttggacag aaagctgggg
     8581 atgaaatatc aggagataaa gtagataaaa tatttctgcc ttttgaagaa aagaaatctt
     8641 cacaactatt agagttagtt gaagaaatac ctattgaatt ttttgaaatc actcgtacta
     8701 tcgtttttga agcagaaaaa ggattagata aaaaactgaa tcctggagtc tatttaacct
     8761 taacagacca cctgcatttt gcagttgaac gcgcacaaaa aggggtaaat attgcaaacc
     8821 gtttgtactg ggaaataaaa atctactatc ccaaagaatt tcaaattggg aagtacgcac
     8881 tcaccctttt gaacgagcaa tatggaattg atcttcctaa agaggaagcc tcaaacattg
     8941 ctttccattt gatcaatgca caatcggaag atcctgatag cggcgctgga ttcaagtatg
     9001 caaaaatgat cggcgggatc gtcaatttgg tccggtattc gttgcagaaa gatatcgata
     9061 ctaattcgat ccattatgcc cgcttcatta cacatgtacg tttctttgta gaaagatatt
     9121 attctgatgc tttgttggat gatggtgagg aagaattcta caatcaaatg tggatcttat
     9181 acccgtctgc catggaaagc gcagctaaga tcaaagacta cattgaaaaa gtttatcata
     9241 aaacaattcc gaacgatgaa gttgtatacc taggtgtcca catcaatcgg ttgatgaaac
     9301 atgctaatgg gtgagcgctc agtaatacta ttgatgcttt taaaatcttt agagatggat
     9361 tatagtaaaa acaaggtgat ttccagctgt ttgttatcta taagagacat ttaaaaagga
     9421 ttaacgtaag ataataaaaa atct
//
LOCUS       DAZKLD010000117         9370 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.117, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000117 DAZKLD010000000
VERSION     DAZKLD010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9370)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9370)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9370
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.117"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..72)
                     /locus_tag="ACG8KR_002297"
     CDS             complement(<1..72)
                     /locus_tag="ACG8KR_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CatA-like O-acetyltransferase"
                     /protein_id="HFQ0201624.1"
                     /translation="MTFNIIELENWDRKEYFEHYFNQQ"
     gene            complement(202..1140)
                     /locus_tag="ACG8KR_002298"
     CDS             complement(202..1140)
                     /locus_tag="ACG8KR_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFQ0201625.1"
                     /translation="MKKIDEKLAAEISLKKPAVSNSRLSNSRLSGQTLGKSQPALSFD
                     AMTIVGNLNKTNAKKLSDFMSVEPQIRLWDILQTKFKAKALQEKVYIEYDKVKADTWD
                     RRNMRVEFNPNKLTHEEMIWLKQNIIDYMEDDGFTRLDLAFDFEDDLSDYYAMTDKAV
                     KKTVFYGRNGKPETKYFGVRDSDRFIRIYNKKQERKDNADVEVVSEHLWRVEVELKRD
                     MVDYWNDCFNDLHILKPAWATLEKINEQAMVYTLLHEESMWGKLSKNTKTKFKKLIRE
                     ISPIDLTELMKSTLKANEKQLQKQIDFWQREFRFWK"
     gene            1631..2848
                     /locus_tag="ACG8KR_002299"
                     /pseudo
     CDS             1631..2848
                     /locus_tag="ACG8KR_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
     gene            2982..3221
                     /locus_tag="ACG8KR_002300"
     CDS             2982..3221
                     /locus_tag="ACG8KR_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002425012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HFQ0201626.1"
                     /translation="MIVGNLGAQKEKRNDTPISAKKDIMGDKTVRVRADLHHIIKIET
                     AKNGGNVKEVMEIRLRSKLKSVLIVHYLKILYNRN"
     gene            3285..3338
                     /locus_tag="ACG8KR_002301"
     CDS             3285..3338
                     /locus_tag="ACG8KR_002301"
                     /inference="COORDINATES: protein motif:HMM:NF018061.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HFQ0201627.1"
                     /translation="MRNVDKTSTVLKQKSSP"
     gene            complement(3376..4548)
                     /locus_tag="ACG8KR_002302"
     CDS             complement(3376..4548)
                     /locus_tag="ACG8KR_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS1542 family transposase"
                     /protein_id="HFQ0201628.1"
                     /translation="MTQVHFTLNNEEVQSIIEHSVKDDVSKNILTTVFNQLMENQRTE
                     YIQADDYERSESRQSQRNGYYERDFTTRVGTLELKVPRTRDGEFSPTVFERYQRNEKA
                     LLASMLEMYVSGVSTRKVSKIVEELCGKSVSKSFVSSLTEQLDPMVNEWQNRSLSGTN
                     YPYLMTDVLYIKVREDHRVLSKSCHIAIGITEGGDREIIGFMIQNEESDDTWSIFFEY
                     LKERGLKGTELIISDAHKGLVSAIRKSFTNASWQRCQVHFLRNIFSSIPKKNSKPFRE
                     AVKAIFKFTDIELARTAKNALVGEYIDQPKYTKACEILDNGFEDAFQYTVIGNSHNRL
                     KSTNLLERLNQEVRRREKIIRIFPNRTSANRLIGAVLMDLHDEWLSSTRKYIKFDQ"
     gene            4700..5395
                     /gene="vanR-A"
                     /locus_tag="ACG8KR_002303"
     CDS             4700..5395
                     /gene="vanR-A"
                     /locus_tag="ACG8KR_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance response regulator
                     transcription factor VanR-A"
                     /protein_id="HFQ0201629.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            5373..6527
                     /locus_tag="ACG8KR_002304"
     CDS             5373..6527
                     /locus_tag="ACG8KR_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance histidine kinase VanS-A"
                     /protein_id="HFQ0201630.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
     gene            6742..7710
                     /locus_tag="ACG8KR_002305"
     CDS             6742..7710
                     /locus_tag="ACG8KR_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HFQ0201631.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            7703..8734
                     /gene="vanA"
                     /locus_tag="ACG8KR_002306"
     CDS             7703..8734
                     /gene="vanA"
                     /locus_tag="ACG8KR_002306"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HFQ0201632.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALVVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            8740..9348
                     /locus_tag="ACG8KR_002307"
     CDS             8740..9348
                     /locus_tag="ACG8KR_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HFQ0201633.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPKRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
BASE COUNT         3017 a         1714 c         1904 g         2735 t
ORIGIN      
        1 ttgctgatta aaatagtgtt caaaatattc ttttctatcc caattttcta attcaataat
       61 attaaaagtc atatataact tcctcctaaa ttttaaattt ttatatttag gaggaataat
      121 cctctgattt tttcatacgt tatgtcacct cgtaaatatt aattatactg aattagcaat
      181 ttttatcaaa taaaacttat tttacttcca aaacctaaat tcacgttgcc aaaaatcaat
      241 ctgcttttgc aattgttttt cgttcgcttt taaagtcgat ttcattaatt ccgttaaatc
      301 aattggagat atttctctaa tcaatttttt aaatttagtc ttagtattct tacttagctt
      361 tccccacata ctttcttcat gcaacaaagt ataaaccata gcttgctcat taattttttc
      421 taaagtagcc cacgcaggtt tcaagatgtg taaatcatta aaacaatcat tccagtaatc
      481 aaccatatct ctttttaatt caacttctac acgccataaa tgttcagaca caacttcaac
      541 atctgcgtta tctttacgtt cttgtttttt attataaatt ctaataaatc tatcactatc
      601 acggacacca aaatattttg tttctggctt gccattacga ccataaaaaa cagttttctt
      661 aactgcttta tcagtcattg catagtaatc gctcaaatca tcttcaaaat caaaagctaa
      721 gtctaatctt gtaaaaccgt catcttccat gtagtcgata atattttgtt ttaaccaaat
      781 catttcttca tgtgtgagtt tattgggatt aaattcaaca cgcatattac gtctatccca
      841 agtatctgct tttactttgt catattcgat ataaactttt tcttgaagtg ccttagcttt
      901 aaactttgtt tgaagtatat cccaaagtcg tatttgtggc tctacactca taaagtcaga
      961 tagcttttta gcattagttt tgttcaaatt tccaacgatt gtcatggcat caaaacttaa
     1021 tgcgggttga gattttccca aagtttgacc acttaaccgg ctattactta accggctatt
     1081 agagacggcc ggttttttta gtgagatttc tgcagctaat ttttcatcaa ttttcttcat
     1141 tttttatcgc tcctaaaata atttttggaa cgaaaaaagg acgagtggaa tcgcaaaata
     1201 aatttcgcct gtatccattc gtccattttg ttttgtattc aaaatcaaaa attgatatac
     1261 taatcatcga atgaataaca agtgtggttt gttattccgg gcgagaacgt tggtagcgtt
     1321 gctcgtcttt ttttatgtct ttttttcgtt aaaactattt taaaactaaa tatatcttaa
     1381 agcaaataaa ttcaaggggc taaaagcgcc ccttaaaaac cccttgcctc tgaaaagttt
     1441 tgtgtttcct ttccgtcggt ttcaaaagct aatttactca aaaaaataga tcaagggtaa
     1501 atgcactcac tgcgttcgcc cttgagctat tttatttcgc aaattttaac gcatttgccg
     1561 acgaaaagat aaaacacata aaaatatacg cagatatatt taaaacgtct tagaacgcaa
     1621 aattttacgt ttaaaagcta tgcaaggact gtatgaagca aaattattga gttatccaag
     1681 aacagataca ccatttatta cagagaacga atttgcttat ttaaaagcga attttggcaa
     1741 atatagcggt tttttaggac ttgatcttga aatggttcaa acagagccta gaaagcgtta
     1801 tgtggacggt agtaaggtac aggaacacca cgccattatc ccaacaaaac aagtacctac
     1861 cgaatctgca ttagcgaaaa tggacgattt acaacgaaaa atttatgctt tagtcgttaa
     1921 aacgaccgtt gccatgtttt tacctgatta cttgtatgaa gaaaccaaga tacaaacgaa
     1981 agtagctgat ttgctgtttc aatcgatcgg aaagacacca aagcaagaag ggtggaaaat
     2041 tcttttcaaa caacaaacca aagaagaaaa agaggacgtt caaacgttac cactcgttat
     2101 cattggcgaa cgtgccgaag ttggtgttaa gagtgttgaa aaagaaacgc aaccgccaaa
     2161 agcttttaca gagggtacat tattaactgc tatgaaaacg gcgaataaaa cggttgatga
     2221 tgaagaagca atcaagattt tacaagaagt tgaggggatt ggaacagaag cgacaagagc
     2281 aagtattatt gaagcgttaa aacaaaaaga atatatccaa gtgattaaga ataagcttgt
     2341 tgtaactgaa aaaggaaaat tattgtgcca ggcagttgaa agtcagcacc ttttaacgag
     2401 tgctgaaatg acggctaaat gggaaacgta tttaaaaaaa atcggtaaaa gagaaggcaa
     2461 tcaagagaac tttattacga atatcaaaaa attcattgtt catttactgg aagctgtacc
     2521 taacgatata gaaaaactaa atttttctga ttaccaggaa cagaaagaaa aagaagcaga
     2581 aaaaagtatt gtaggaaaat gtcctaagtg tggcaacaat attgtattaa aaaaatcgtt
     2641 ttatggttgt tcaaattatc ctgaatgtaa gtttacttta gctgaacatt ttagaaagaa
     2701 aaaactaacc aaaacgaatg taaaagaatt actggaggga aaagaaaccc tggtaaaagg
     2761 aatcaaaaac aaagagaaaa agccctacaa tgccgttgta aaaattgggg aaaagggata
     2821 tattgatttt atatctttct caaaataaac ataaaagccc tttaaagagg gcttttatat
     2881 attaatcaca aatcacttat cacaaatcac ttatcacaaa tcacaagtga tttgtgattg
     2941 ttgatgataa aataagaata agaagaaata gaaagaagtg agtgattgtg ggaaatttag
     3001 gcgcacaaaa agaaaaacga aatgatacac caatcagtgc aaaaaaagat ataatgggag
     3061 ataagacggt tcgtgttcgt gctgacttgc accatatcat aaaaatcgaa acagcaaaga
     3121 atggcggaaa cgtaaaagaa gttatggaaa taagacttag aagcaaactt aagagtgtgt
     3181 tgatagtgca ttatcttaaa attttgtata ataggaattg aagttaaatt agatgctaaa
     3241 aatttgtaat taagaaggag ggattcgtca tgttggtatt ccaaatgcgt aatgtagata
     3301 aaacatctac tgttttgaaa cagaagtcaa gtccataatc ctgtgtaaaa tactatacaa
     3361 tgttttaccg gtctctcatt gatcaaactt aatatatttt cttgtagaac tgagccattc
     3421 gtcatgaagg tccataagga cagctccaat taatcgattg gcagacgttc ggttgggaaa
     3481 aatccgaata atcttttctc ttctgcggac ttcctggttc agtcgttcaa gaaggttggt
     3541 gctttttagc cgattatgac tatttccgat aaccgtgtat tgaaaggcat cttcgaagcc
     3601 attatccaat atttcgcagg cttttgtata tttaggctgg tcgatatatt caccgactaa
     3661 ggcattctta gctgttcgag cgagttcaat atccgtaaac ttaaagatcg cttttactgc
     3721 ttctctaaac ggttttgaat tcttttttgg aatggaactg aagatgtttc ttaaaaaatg
     3781 gacctggcat ctctgccaac ttgcgttggt aaatgactta cgaatcgcag acactaggcc
     3841 tttatgggca tcagaaatga tgagttctgt cccctttagg ccgcgttctt ttaagtattc
     3901 aaagaagatg gaccatgtgt catcactttc ttcattttga atcatgaagc caatgatttc
     3961 acggtcgcca ccttctgtta tcccgatcgc aatatggcag cttttagaaa gcactcgatg
     4021 gtcctcacgg acttttatgt agagaacatc agtcatcaga taaggataat tcgtacctga
     4081 gagtgaacgg ttctgccatt cgttgaccat cgggtctaac tgctcagtca ggctagaaac
     4141 aaaagatttc gatacagatt ttccacatag ctcttcaaca atctttgaaa ctttacgtgt
     4201 cgaaacgccc gaaacataca tctcaagcat tgaagcgagc agtgcctttt catttcgctg
     4261 ataacgctca aacaccgtcg gtgaaaattc accatcacgt gttctaggca cttttaattc
     4321 gagtgtaccc acacgagtcg taaagtctcg ctcataatag ccatttcttt gactctgacg
     4381 actttctgaa cgttcatagt catcggcttg aatatattct gttcgttgat tttccatcaa
     4441 ttggttgaaa acagtggtta aaatattttt agaaacatca tcttttaccg aatgttcaat
     4501 aatactttga acctcttcgt tgttcagtgt aaaatgtact tgggtcatgt aacgtcctcc
     4561 tgggtatgtt tttgtggtta aaaacattgt accgtaaaag ggctgttaca tggcctttta
     4621 tcttttacac aattatatgg actttatcct ataatctgat aaattattaa gggcaaactt
     4681 atgtgaaagg gtgataacta tgagcgataa aatacttatt gtggatgatg aacatgaaat
     4741 tgccgatttg gttgaattat acttaaaaaa cgagaattat acggttttca aatactatac
     4801 cgccaaagaa gcattggaat gtatagacaa gtctgagatt gaccttgcca tattggacat
     4861 catgcttccc ggcacaagcg gccttactat ctgtcaaaaa ataagggaca agcacaccta
     4921 tccgattatc atgctgaccg ggaaagatac agaggtagat aaaattacag ggttaacaat
     4981 cggcgcggat gattatataa cgaagccctt tcgcccactg gagttaattg ctcgggtaaa
     5041 ggcccagttg cgccgataca aaaaattcag tggagtaaag gagcagaacg aaaatgttat
     5101 cgtccactcc ggccttgtca ttaatgttaa cacccatgag tgttatctga acgagaagca
     5161 gttatccctt actcccaccg agttttcaat actgcgaatc ctctgtgaaa acaaggggaa
     5221 tgtggttagc tccgagctgc tatttcatga gatatggggc gacgaatatt tcagcaagag
     5281 caacaacacc atcaccgtgc atatccggca tttgcgcgaa aaaatgaacg acaccattga
     5341 taatccgaaa tatataaaaa cggtatgggg ggttggttat aaaattgaaa aataaaaaaa
     5401 acgactattc caaactagaa cgaaaacttt acatgtatat cgttgcaatt gttgtggtag
     5461 caattgtatt cgtgttgtat attcgttcaa tgatccgagg gaaacttggg gattggatct
     5521 taagtatttt ggaaaacaaa tatgacttaa atcacctgga cgcgatgaaa ttatatcaat
     5581 attccatacg gaacaatata gatatcttta tttatgtggc gattgtcatt agtattctta
     5641 ttctatgtcg cgtcatgctt tcaaaattcg caaaatactt tgacgagata aataccggca
     5701 ttgatgtact tattcagaac gaagataaac aaattgagct ttctgcggaa atggatgtta
     5761 tggaacaaaa gctcaacaca ttaaaacgga ctctggaaaa gcgagagcag gatgcaaagc
     5821 tggccgaaca aagaaaaaat gacgttgtta tgtacttggc gcacgatatt aaaacgcccc
     5881 ttacatccat tatcggttat ttgagcctgc ttgacgaggc tccagacatg ccggtagatc
     5941 aaaaggcaaa gtatgtgcat atcacgttgg acaaagcgta tcgactcgaa cagctaatcg
     6001 acgagttttt tgagattaca cggtataacc tacaaacgat aacgctaaca aaaacgcaca
     6061 tagacctata ctatatgctg gtgcagatga ccgatgaatt ttatcctcag ctttccgcac
     6121 atggaaaaca ggcggttatt cacgcccccg aggatctgac cgtgtccggc gaccctgata
     6181 aactcgcgag agtctttaac aacattttga aaaacgccgc tgcatacagt gaggataaca
     6241 gcatcattga cattaccgcg ggcctctccg gggatgtggt gtcaatcgaa ttcaagaaca
     6301 ctggaagcat cccaaaagat aagctagctg ccatatttga aaagttctat aggctggaca
     6361 atgctcgttc ttccgatacg ggtggcgcgg gacttggatt ggcgattgca aaagaaatta
     6421 ttgttcagca tggagggcag atttacgcgg aaagcaatga taactatacg acgtttaggg
     6481 tagagcttcc agcgatgcca gacttggttg ataaaaggag gtcctaagag atgtatataa
     6541 ttttttagga aaatctcaag gttatcttta ctttttctta ggaaattaac aatttaatat
     6601 taagaaacgg ctcgttctta cacggtagac ttaataccgt aagaacgagc cgttttcgtt
     6661 cttcagagaa agatttgaca agattaccat tggcatcccc gttttatttg gtgcctttca
     6721 cagaaagggt tggtcttaat tatgaataac atcggcatta ctgtttatgg atgtgagcag
     6781 gatgaggcag atgcattcca tgctctttcg cctcgctttg gcgttatggc aacgataatt
     6841 aacgccaacg tgtcggaatc caacgccaaa tccgcgcctt tcaatcaatg tatcagtgtg
     6901 ggacataaat cagagatttc cgcctctatt cttcttgcgc tgaagagagc cggtgtgaaa
     6961 tatatttcta cccgaagcat cggctgcaat catatagata caactgctgc taagagaatg
     7021 ggcatcactg tcgacaatgt ggcgtactcg ccggatagcg ttgccgatta tactatgatg
     7081 ctaattctta tggcagtacg caacgtaaaa tcgattgtgc gctctgtgga aaaacatgat
     7141 ttcaggttgg acagcgaccg tggcaaggta ctcagcgaca tgacagttgg tgtggtggga
     7201 acgggccaga taggcaaagc ggttattgag cggctgcgag gatttggatg taaagtgttg
     7261 gcttatagtc gcagccgaag tatagaggta aactatgtac cgtttgatga gttgctgcaa
     7321 aatagcgata tcgttacgct tcatgtgccg ctcaatacgg atacgcacta tattatcagc
     7381 cacgaacaaa tacagagaat gaagcaagga gcatttctta tcaatactgg gcgcggtcca
     7441 cttgtagata cctatgagtt ggttaaagca ttagaaaacg ggaaactggg cggtgccgca
     7501 ttggatgtat tggaaggaga ggaagagttt ttctactctg attgcaccca aaaaccaatt
     7561 gataatcaat ttttacttaa acttcaaaga atgcctaacg tgataatcac accgcatacg
     7621 gcctattata ccgagcaagc gttgcgtgat accgttgaaa aaaccattaa aaactgtttg
     7681 gattttgaaa ggagacagga gcatgaatag aataaaagtt gcaatactgt ttgggggttg
     7741 ctcagaggag catgacgtat cggtaaaatc tgcaatagag atagccgcta acattaataa
     7801 agaaaaatac gagccgttat acattggaat tacgaaatct ggtgtatgga aaatgtgcga
     7861 aaaaccttgc gcggaatggg aaaacgacaa ttgctattca gctgtactct cgccggataa
     7921 aaaaatgcac ggattacttg ttaaaaagaa ccatgaatat gaaatcaacc atgttgatgt
     7981 agcattttca gctttgcatg gcaagtcagg tgaagatgga tccatacaag gtctgtttga
     8041 attgtccggt atcccttttg taggctgcga tattcaaagc tcagcaattt gtatggacaa
     8101 atcgttgaca tacatcgttg cgaaaaatgc tgggatagct actcccgcct tttgggttat
     8161 taataaagat gataggccgg tggcagctac gtttacctat cctgtttttg ttaagccggc
     8221 gcgttcaggc tcatccttcg gtgtgaaaaa agtcaatagc gcggacgaat tggactacgc
     8281 aattgaatcg gcaagacaat atgacagcaa aatcttaatt gagcaggctg tttcgggctg
     8341 tgaggtcggt tgtgcggtat tgggaaacag tgccgcgtta gttgttggcg aggtggacca
     8401 aatcaggctg cagtacggaa tctttcgtat tcatcaggaa gtcgagccgg aaaaaggctc
     8461 tgaaaacgca gttataaccg ttcccgcaga cctttcagca gaggagcgag gacggataca
     8521 ggaaacggca aaaaaaatat ataaagcgct cggctgtaga ggtctagccc gtgtggatat
     8581 gtttttacaa gataacggcc gcattgtact gaacgaagtc aatactctgc ccggtttcac
     8641 gtcatacagt cgttatcccc gtatgatggc cgctgcaggt attgcacttc ccgaactgat
     8701 tgaccgcttg atcgtattag cgttaaaggg gtgataagca tggaaatagg atttactttt
     8761 ttagatgaaa tagtacacgg tgttcgttgg gacgctaaat atgccacttg ggataatttc
     8821 accggaaaac cggttgacgg ttatgaagta aatcgcattg tagggacata cgagttggct
     8881 gaatcgcttt tgaaggcaaa agaactggct gctacccaag ggtacggatt gcttctatgg
     8941 gacggttacc gtcctaagcg tgctgtaaac tgttttatgc aatgggctgc acagccggaa
     9001 aataacctga caaaggaaag ttattatccc aatattgacc gaactgagat gatttcaaaa
     9061 ggatacgtgg cttcaaaatc aagccatagc cgcggcagtg ccattgatct tacgctttat
     9121 cgattagaca cgggtgagct tgtaccaatg gggagccgat ttgattttat ggatgaacgc
     9181 tctcatcatg cggcaaatgg aatatcatgc aatgaagcgc aaaatcgcag acgtttgcgc
     9241 tccatcatgg aaaacagtgg gtttgaagca tatagcctcg aatggtggca ctatgtatta
     9301 agagacgaac cataccccaa tagctatttt gatttccccg ttaaataaac ttttaaccgt
     9361 tgcacggaca
//
LOCUS       DAZKLD010000118         9292 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.118, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000118 DAZKLD010000000
VERSION     DAZKLD010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9292)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9292
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.118"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(92..1141)
                     /gene="recA"
                     /locus_tag="ACG8KR_002308"
     CDS             complement(92..1141)
                     /gene="recA"
                     /locus_tag="ACG8KR_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HFQ0201634.1"
                     /translation="MADDRKAALDAALKKIEKSYGKGSIMKLGEKIDQQISTIPSGSL
                     ALDVALGVGGYPRGRIIEVYGPESSGKTTVALHAIAEVQKNGGTAAFIDAEHALDPQY
                     AQKLGVNIDELLLSQPDTGEQGLEIADALVSSGAVDIVVVDSVAALVPRAEIDGEMGD
                     SHVGLQARLMSQALRKLSGSINKTKTIAIFINQIREKVGVMFGNPEITPGGRALKFYA
                     TIRLEVRRAEQLKQGTDIVGNRTKIKVVKNKVAPPFKIAEVDVMYGLGISQEGELLDM
                     AVEKDIVDKSGAWYSYKEDRIGQGRENAKIYMANHPEMMAEVSALVRAAYGIGEEVAV
                     PEDEKGQEELPLVEE"
     gene            complement(1250..2491)
                     /locus_tag="ACG8KR_002309"
     CDS             complement(1250..2491)
                     /locus_tag="ACG8KR_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HFQ0201635.1"
                     /translation="MKAEIIAVGTELLLGQVVNTNATFLSEQLADLGIEVYYQTVVGD
                     NQQRLEELIALAETRSELILLCGGLGPTEDDLTKEATAAHLGKSLIQNTEGYKKLLAY
                     FETTHRKMTKNNLQQSQIIEGGVPLPNRTGLALGTFYQTDTHAYILLPGPPNELKPMF
                     VEQVRPLLEERFPSEEKLISKVLRFYGIGESRLVTELKDLIETQINPTIAPYAKPNEV
                     TLRLTVKTNDVQAGNQALLALEEKIQERVGEYFYGYGDDNSLAKVVVELLKENKQTVT
                     AAESLTAGAFQAALGDIAGVSEVFPGGFVTYSLQTKAGFLEIDPELLAEYGTVSKECV
                     EQMAIQAREIARTDYSVAFTGVAGPDTLEGQSAGTVWIAISSKDGVVSEKYHFARDRA
                     AIRYNAVMQGFDLLRKQLLRK"
     gene            complement(2731..3309)
                     /gene="pgsA"
                     /locus_tag="ACG8KR_002310"
     CDS             complement(2731..3309)
                     /gene="pgsA"
                     /locus_tag="ACG8KR_002310"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HFQ0201636.1"
                     /translation="MNLPNKLTVLRICMIPIFILVVGLPLDWGQFNVGDTTLAVTQLV
                     GAVIFALASITDWLDGKIARARGLVTNFGKFADPLADKMLVMTAFIMLVDQGKAPGWV
                     VAIIVCRELAVTGLRLLLVEHGEVMAAAWPGKVKTATQMVAIILLFINNIPFSLIGIP
                     VDQIMLYACLIFTIYSGVDYFIKNSGVFKGSM"
     gene            complement(3518..4441)
                     /locus_tag="ACG8KR_002311"
     CDS             complement(3518..4441)
                     /locus_tag="ACG8KR_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RodZ domain-containing protein"
                     /protein_id="HFQ0201637.1"
                     /translation="MLRRIKQTGEDERVAFETIGEKLRQARLNKKISLDELQQITKIQ
                     KRYLEAIDNDDFDQLPGKFYVRAFIRQYAEAVGEDGDHLVDVFDGKSKLTGSVPERPQ
                     PETVSTSRKAMHQEESHPSRFWTSLPVILLGLVALAIVVVVGYMTWQDRQADPIIGDT
                     ASSLKVEGSVEESSSSEQSTQTKESSTVESTTKSSEEKKMAIATGEDSGSAIAVNITD
                     ATKPVTLEFTAVNRVWLGVLVDNAYVYQGTLAANETQSTVLPETATNATITIGAASNA
                     TIKANGESVPVNPGENNQSPKNVNLTLQYAE"
     gene            complement(5180..6466)
                     /locus_tag="ACG8KR_002312"
     CDS             complement(5180..6466)
                     /locus_tag="ACG8KR_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFQ0201638.1"
                     /translation="MNKTEYEQINETLYHEVLPNGLTVYLLPKNDYHKTYGLFSTNYG
                     SIDNEFIPYGEKEKVKVPDGIAHFLEHKLFEKEDGDVFQLFGKQGASANAFTSFTKTS
                     YLFSTTDQVEKNLTTLIDFVQAPYFTEETVNKEKGIIGQEIQMYEDDPNWRMFFGILN
                     NLYPTHPLHIDIAGTVESIDKITAQDLYTCYRTFYQPSNMVLFVVGKMEPEKLMKLIR
                     ENQEAKNFPPKQEIVRYFPENTKEIIKQSALEAAITRDKFVLGIKGLDTLPQEGTELL
                     RYKTAINLLFQMILGNTSRNYLAMYNQGIIDDSFGFEFSLDREFHFADFSGDTDEPEK
                     AAEKVKEIILGFADDPEVSETNLDLLKKKMLGQYFQSLNSIEYIANQFTQSLFGDRTL
                     FDLPEIIDSIQMKDVLAAGEAFISEEAFSEFYMRPK"
     gene            complement(6459..7727)
                     /locus_tag="ACG8KR_002313"
     CDS             complement(6459..7727)
                     /locus_tag="ACG8KR_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFQ0201639.1"
                     /translation="MVYQLAEGVNLHVLPTKQYKTIRIFIRFTARLQQEVITKRSLLS
                     SMLETNSLNYPDQTKLSAQLAELYGASFGLSVRKKGNLHWLNAGISFVNGEYVNDPNL
                     FSQAVDFLKEVLFYPNIKNHQFDQLTFDLEKNNLRLYLESLKEDKQTFASYALQELYF
                     ENSPEQKIPGLGVVEDLDKITASSLASYYQEMMANDQIDIFVVGDVDPDKAAEAVGQL
                     PFESRETVYPDLFYTQPQVNIVKERQVREPIVQAKLNLAYQTNVYYDEPERFALMVFN
                     GLFGGFPHSKLFMNVREKESLAYYASSSVDTFRGFMSVQTGIDEKNRNQVLHLIHEQL
                     ESLRNGEITELELSQTKAMLRNQYLLSLDSPQAAIEASFLDSWLPETKLSDEEWLKRM
                     ESVTIKEIQQVAEQIELQAIFFLAGGNANE"
     gene            7979..8440
                     /gene="mscL"
                     /locus_tag="ACG8KR_002314"
     CDS             7979..8440
                     /gene="mscL"
                     /locus_tag="ACG8KR_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HFQ0201640.1"
                     /translation="MIKEFKEFIMRGDVLDLAVGVVIGSAFTSIVNQVVQGLITPLIG
                     WFVAMITGTSDLEGALSILDWSPTKGVTFAFGNVISAIITFLITGFVLFLVVKAANRA
                     KNLRSKEEQVEVEETPTAEDYLSDIRDLLAKQVAEDNTHPVEQTNLSDTSK"
     gene            complement(8499..8945)
                     /locus_tag="ACG8KR_002315"
     CDS             complement(8499..8945)
                     /locus_tag="ACG8KR_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0201641.1"
                     /translation="MEYQVKKFEELSTAEFYAIVKERIAVFVVEQQCPYQEVDDIDGQ
                     AWHTFFQDTDGKILAYTRVYEEAESIHFGRVLVHQDNRKDGLGRKIVQETINMIQKNF
                     PEKPIVIGAQEYLQAFYESFGFKAISDVYLEDDIPHIDMKKEQVAS"
     gene            complement(9038..9283)
                     /locus_tag="ACG8KR_002316"
     CDS             complement(9038..9283)
                     /locus_tag="ACG8KR_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HFQ0201642.1"
                     /translation="MPTTLASIKKDLEGRIGSPIMLVAQTGRKRQTERRGVLTETYPS
                     VFVVDLDPEENSFERVSYSYSDVLTRTVEIEFLTEAV"
BASE COUNT         2560 a         2014 c         1658 g         3060 t
ORIGIN      
        1 aataactcac gatttcttta ttcaataaaa aaacggcagg tcaaggagag tacctactct
       61 tcagaccagc cgtttttgtt tatcataatt tttattcttc tactaaaggc aattcttctt
      121 gtcctttttc atcttctggg acagcgactt cttcaccaat accataagct gctctaacta
      181 gagcagaaac ttctgccatc atttcaggat gattagccat atagatttta gcgttttccc
      241 gtccttgacc aatccgatct tctttataag aataccaagc accgctttta tcaacaatgt
      301 ctttttctac tgccatgtct agaagttctc cttcttgtga gatccctaaa ccatacatga
      361 catctacttc agcgattttg aaaggaggtg ctactttgtt ttttacgact ttgatctttg
      421 tacggttacc aacaatatct gtaccttgtt tcaattgttc tgcacgacgt acttccaaac
      481 ggatcgttgc gtagaatttc aatgcgcgtc ctccaggagt gatttctgga ttcccaaaca
      541 tcacgcctac tttttcacgg atttggttga tgaaaatagc gattgttttt gttttgttga
      601 tcgaaccaga gagtttacgc aatgcttgag acatcaaacg tgcttgtaac ccgacatgtg
      661 agtcacccat ttcgccgtcg atttctgctc gtggaactaa agcagcaact gaatcgacta
      721 ctacgatatc tacagcccca cttgatacta aagcatcagc gatctctaga ccttgttctc
      781 ctgtgtcagg ctgtgaaaga agtagttcat cgatattcac acctaatttt tgtgcatatt
      841 gcggatctaa cgcatgctca gcatcaatga aagcggccgt tccgccattt ttttgtactt
      901 ctgcaatagc gtgtagtgca actgttgttt tacctgaact ttcaggtcca tatacttcga
      961 tgatacgtcc acgggggtaa ccgccgacac ctaaagcgac atctaacgca agagagccac
     1021 ttggaattgt agagatttgt tggtcgattt tttcgcccaa tttcataata gagcctttac
     1081 catagctctt ttcaattttt ttcagtgcag catctaaggc tgctttacga tcatctgcca
     1141 atacgataat cctccttata tttacaaatc agagcaacat ttcattatcc ttatcatatc
     1201 ttttttagtg aaaaaaagca agaaaaaacc gaacaaatat tcgcatttat cattttctta
     1261 aaagctgttt tctcaaaaga tcaaatcctt gcataaccgc attatatctg atagcagcac
     1321 gatctctggc aaagtgatat ttttccgaaa ccacgccgtc tttagatgaa atagcgatcc
     1381 atactgttcc tgcagattga ccttctaacg tgtccggtcc agccactccg gtgaaagcta
     1441 cactgtaatc tgttcttgct atctcacgtg cttgaatggc catctgctcc acacattctt
     1501 tgctgactgt tccatattct gctaatagct ccggatcaat ctccagaaaa ccagcttttg
     1561 tttggagcga gtaggtaacg aatccgcctg gaaatacttc agatacacca gcaatgtcgc
     1621 ctaatgcggc ttggaaggcg ccggctgtca aactttccgc tgctgttaca gtctgcttgt
     1681 tttctttcag tagttccact acgacttttg ctagcgaatt atcatcgccg tatccataaa
     1741 aatattcccc tactcgttct tggatttttt cttctaatgc taaaagagct tgattccctg
     1801 cctgaacatc atttgttttc actgtcagtc gtaatgtcac ttcatttggt ttggcatatg
     1861 gtgcgatcgt tggattgatc tgtgtctcaa tcagatcttt tagctcagtg accagtcgcg
     1921 attcaccaat cccataaaag cgcaaaactt tagaaattag tttttcttca gaaggaaaac
     1981 gttcctctag taaaggacgt acttgttcca caaacattgg tttcaactca ttaggcggac
     2041 cgggaagaag gatatacgca tgtgtatctg tttgataaaa agtccctaaa gcaagccctg
     2101 tacgatttgg tagaggaacc ccgccttcga taatctggga ctgttgcaga ttgtttttgg
     2161 tcatttttcg atgggttgtt tcaaaatatg ccaataattt tttgtatcct tccgtatttt
     2221 gtatgagaga cttaccgagg tgagccgcag ttgcttcttt tgtcaggtca tcttctgtcg
     2281 gtcctaatcc gccacataag agtatgagtt cacttcttgt ttcagctaga gcaatcaact
     2341 cttctaaacg ctgctgatta tctcctacaa ctgtttgata ataaacttcg atacctagat
     2401 cagctagctg ttctgacaag aaagtggcat tcgtattcac gacttgtcct agtaaaagtt
     2461 ctgttccgac agctattatt tctgctttca ttacttcccg ctcccttata tagtatacgc
     2521 tgtattttta tattttttta tttaaaaaga gggcaaggag ctgtgacaaa aatcttgtca
     2581 cagctccttg ttccaaataa acagtgttct cctgccaaaa cctcggtcat aagtcacaat
     2641 atacataaaa catgaaaagc tgttttttga aattatccct tgtgactcag agttagacgg
     2701 caggctcaca acctctccac taaacgatac ttacattgat cctttgaaca caccgctgtt
     2761 tttgatgaag taatccacac ctgaatagat agtaaagatc agacaagcat aaagcatgat
     2821 ttgatctact ggaataccga tcaggctgaa tgggatatta ttgataaaca gcaagataat
     2881 agcaaccatt tgtgtagccg tcttgacttt tcccggccaa gctgcagcca tgacttctcc
     2941 atgttcgaca agtagtaaac gcaagcctgt gacggctaat tcacgacaga caatgatcgc
     3001 tactacccaa ccaggggcct ttccttggtc aaccaacatg ataaaagcag tcataactag
     3061 cattttatct gccaacgggt ccgcaaactt gccaaaatta gtgacaaggc ctcgcgcacg
     3121 ggcaattttt ccatctaacc agtctgtgat actagctaaa gcaaaaataa ctgcacctac
     3181 tagctgtgtg acagccagtg tggtgtctcc tacgttaaat tgtccccaat ctaaaggtaa
     3241 acctacaacc aaaatgaaaa tcgggatcat acaaattcta agtacagtta atttatttgg
     3301 taaattcaca ctatcgactc ctttcttccc tattctacgt gactatcagc gaagtttcca
     3361 ccgatattat ttacttttcc aaatgaattt attcaaagga gtatttttat tgaaattgat
     3421 ctttttgaat taacgaagaa ggaccgcgac acagatttgt cacgatcctt cttttttata
     3481 cctacaagta aacagatgcg taaactaatt tatacgatca ttctgcgtat tgaagagtaa
     3541 ggttcacatt ttttggactc tgattgtttt cccctggatt aactggtacg gattctccat
     3601 tcgctttgat cgttgcattg cttgctgcac caatcgtgat ggtcgcattt gttgctgttt
     3661 ccggtaacac ggtactctgt gtttcatttg ctgccagtgt cccttgataa acataggcat
     3721 tgtctactag cacacctaac catacacgat tgacagcagt aaattcaagg gttacaggct
     3781 tggtagcgtc tgtgatattt actgcgatgg cactgccaga atcttctcct gtagctattg
     3841 ccattttctt ttcttcactt gattttgttg ttgattctac tgtagaagac tctttggtct
     3901 gcgtgctttg ctctgaggaa gaggactcct cgactgatcc ttctacttta agcgaagaag
     3961 ctgtgtctcc gattattgga tcagcttgac gatcttgcca agtcatatag cccacgacaa
     4021 cgacaatcgc caaagcaacc aatcctaata aaatgacagg aagacttgtc caaaagcgac
     4081 ttggatgcga ttcttcctga tgcattgctt ttctagaagt acttactgtt tcaggctgcg
     4141 gacgttcagg gaccgatcct gtcaatttgc ttttcccatc aaaaacatcg actaaatggt
     4201 ctccatcttc tcctacagct tcagcgtatt gccgaataaa tgcccgcacg taaaatttac
     4261 caggcaattg gtcaaaatca tcattgtcga ttgcttccaa ataacgtttt tgaatttttg
     4321 taatctgctg taattcatct aaagatattt ttttattcag gcgtgcttgc cgtaattttt
     4381 ctccgatggt ttcaaaagcc actcgttcat cttctccagt ctgtttgatt ctccgaagca
     4441 tctgctgttg caacgaaaga cgttatgttt gggtgctgtg acaaaagtca tgtcacagct
     4501 ccttttttcc gaataaacat tgttctcctg ccaactcctc ggtcacaagt cacaatatcc
     4561 aataaatata agaagctgtt ttttgaactt gttccttgtg actcggagtt aaacggcagg
     4621 ctcacgacct ctttcttaaa cggtgttcaa tcagctgttc ctcggtatta gctcaaaaac
     4681 cggcaaaata taaggagata tttttccgta tgtttctcta atactcaggt caaaacgctg
     4741 atttcacaac ctcttcctta aacggtgttc aaaagcttgc atctgcggta ataagtccaa
     4801 ccaagttaaa aacattaaga agctgttttt acttggctgt tcttattact tgatgcaggg
     4861 cagctttttc acaacctctt ggtacacggt gttccagaag tagcttcttc ggcaataagc
     4921 cgaaatatca caaaaatttg agaagcaatt ttcgtaaatt ccttcttatt tcttgaagct
     4981 gaccacttct gtcacaacct ctttgattac ttcgtttgat tttattttaa tataagttga
     5041 ttaaaagaga gcatgatttg ttttattttc tcactaattt ttccaacaca actaagaata
     5101 ccataaaagc tgattttgga ggaggttcct actacaagtc taaaacgaaa tttaagactt
     5161 ttcctcataa aatactggtt tattttggtc tcatgtaaaa ctcgctgaag gcctcttcac
     5221 ttataaatgc ttccccagct gccagtacgt ctttcatttg gatcgaatcg atgatttcag
     5281 gaagatcaaa taaagttcga tcgccaaata aactttgcgt aaattgattg gcaatatatt
     5341 cgatcgaatt taatgattgg aaatattgtc caagcatttt cttcttcagc aaatcgagat
     5401 ttgtttctga cacttccgga tcatcagcga atccaagaat gatttctttg actttttctg
     5461 ctgctttctc tggttcgtcc gtatctccgc tgaagtccgc aaagtgaaat tcacgatcca
     5521 agctgaattc aaaaccaaag ctgtcatcga taattccttg attgtacatc gctagatagt
     5581 ttcgagaagt attgcctaaa atcatttgga acaacagatt gattgctgtt ttatagcgta
     5641 acagttctgt tccttcttgc ggcaatgtgt ctaatccttt aataccaagt acaaatttat
     5701 ctcgtgtaat ggctgcttcc aatgcacttt gtttgatgat ttcttttgtg ttttcaggga
     5761 aataacgtac aatttcttgc tttggaggga aattttttgc ttcttggttt tcccgaataa
     5821 gtttcatcaa tttctcaggc tccatcttac caactacaaa taaaaccata ttgcttggct
     5881 gataaaacgt ccgatagcac gtatacaagt cttgagctgt gattttgtcg atactttcaa
     5941 ccgtgcccgc aatatcgata tgcaatgggt gtgtaggata caagttattt agtatgccaa
     6001 aaaacatccg ccagttaggg tcgtcttcat acatttggat ttcctgaccg atgattcctt
     6061 tttccttgtt tacagtttct tctgtgaagt aaggtgcttg tacaaaatcg atcagagtcg
     6121 tcagattctt ttctacctga tccgtcgtag aaaacagata gctggttttc gtaaaacttg
     6181 tgaaagcatt ggcggaagct ccttgtttcc caaataattg aaagacatca ccatcttctt
     6241 tttcaaacaa cttatgttct aaaaaatggg caattccatc tggaactttc accttttctt
     6301 tttcaccata gggaataaat tcgttatcga tcgaaccata attcgtactg aacaaaccat
     6361 atgtcttgtg atagtcattt ttaggcaaca aatacacggt caaaccgttt ggcagtactt
     6421 catgataaag tgtttcattg atttgctcat attctgtttt attcatttgc atttcctcct
     6481 gctaaaaaga agattgcctg caattcgatt tgctcagcta cttgttgtat ctctttgatc
     6541 gttacgctct ccatacgttt caaccattct tcatcagata attttgtttc cggcagccag
     6601 ctgtccaaaa aagaagcttc gattgccgct tgcggagagt ctagagaaag cagatattgg
     6661 tttctcagca ttgcttttgt ctgagacagt tccaactctg ttatttcacc atttcgcaaa
     6721 ctttctaatt gctcgtgaat cagatgaagt acttgatttc ggttcttttc atcgataccg
     6781 gtttggacac tcatgaatcc tcggaacgta tccacgctac tagaggcata ataagctaga
     6841 ctttctttct ctctgacatt catgaaaagt ttagagtgag gaaaaccgcc aaataaaccg
     6901 ttaaagacca ttagcgcaaa acgttctggt tcatcgtaat acacatttgt ctgataagct
     6961 aggttcagct ttgcttgaac gattggctca cgtacttgtc gttccttcac gatattcact
     7021 tgaggttggg tataaaacag atctggataa actgtttccc gcgactcaaa tggtaattgc
     7081 ccaactgctt cagctgcttt gtccggatca acatcgccga caacaaaaat gtcgatttga
     7141 tcatttgcca tcatttcttg atagtaggac gctagcgaac tagcagtgat cttgtccaaa
     7201 tcttcgacta cgcccaaccc tgggatcttt tgttccgggc tgttttcaaa ataaagttct
     7261 tgcaaggcat aggaagcaaa agtttgttta tcttccttca aactttccaa atatagacgc
     7321 agattgttct tttccaagtc aaacgtcaac tgatcaaact gatgattttt aatatttgga
     7381 taaaataaaa cctcttttaa aaaatctacc gcttgagaaa aaaggtttgg atcattgaca
     7441 tattcaccat tcacaaaact gatccctgcg tttagccaat gtaaatttcc tttttttcta
     7501 actgataatc caaagcttgc gccataaagc tctgcaagtt gcgcacttaa ctttgtctga
     7561 tccggataat tcaaactatt tgtttccaac atgctgctga gaagtgaacg tttcgtgatg
     7621 acttcttgtt gcagacgcgc agtaaaacgg ataaaaatcc ggatcgtttt gtattgtttt
     7681 gttggtaaga catgaagatt cacgccttct gccaattgat acaccatatt tatatgctcc
     7741 ttttcacagt tgattgcccg cttactcttt taatgggtca acctgccttt tttacttatt
     7801 cctctgcttg aaatttataa gttcaggcaa ttcgccgttt tttctaaatg atgttacact
     7861 ttatagtata gcatagatgt tttttatcaa aagaaacaga cggctaaaag cgtatattgt
     7921 ttttttattt tttatttttc gatatactaa agacaaataa aagaagggag ggtcattcat
     7981 gatcaaagaa ttcaaagagt tcatcatgcg tggagatgtc ttggatcttg ctgtcggcgt
     8041 tgttattggt agtgcgttta caagcatcgt aaaccaagtg gtgcaaggct tgatcacacc
     8101 actaataggt tggtttgtcg caatgatcac aggtacttct gatttagaag gagcactttc
     8161 aattcttgac tggtcgccga caaaaggcgt cacatttgcg tttggtaacg ttattagtgc
     8221 tatcattaca tttctgatca ctggttttgt cttattctta gttgtcaaag cggcaaaccg
     8281 tgcgaagaat ttacgctcca aagaagagca agtggaagtg gaagaaactc ctactgcaga
     8341 agattatctt agtgacatac gcgatcttct tgccaaacaa gtggcagaag acaatacaca
     8401 tccagtagag caaacaaatc tttccgatac ttctaaataa taaagaatct aaaaaaaaga
     8461 aagccgattt tcagtgtcgg ctttcttttt ttagattctt aacttgctac ttgttccttt
     8521 ttcatgtcga tatgtggaat atcatcttcc aaatacacat ctgagattgc cttgaatcca
     8581 aacgactcat aaaaagcttg taggtattct tgtgcgccga tcacgattgg tttttctgga
     8641 aaatttttct ggatcatgtt aatcgtttct tgaacaatct ttcttcctaa accatcttta
     8701 cggttgtcct gatgaacaag gacacgtcca aaatggatac tctccgcttc ttcatatact
     8761 cgcgtgtagg caaggatctt cccatcagta tcttgaaaaa acgtatgcca agcttgtcca
     8821 tcaatgtcat caacttcttg ataaggacac tgttgttcca caacaaaaac tgcgattcgt
     8881 tctttgacga tcgcgtaaaa ctctgctgtt gacaattctt caaatttctt tacctgatat
     8941 tccactaagc aaacgccccc tgctttttta ttcttagcat accgattcgc acacaaaaaa
     9001 gcaaacgaac cttacgaatc tcaggctcgt ttgcttttta aactgcttca gttaaaaatt
     9061 cgatctcaac tgtccgagta agtacatctg aatagctgta agatactcgt tcaaaagaat
     9121 tttcttcagg atccaaatca acaacgaata ctgaaggata ggtttctgtt aaaacgccgc
     9181 ggcgttctgt ttgacgcttt cttcctgtct gagcaaccaa catgattggg ctgccgatac
     9241 gaccttctaa atctttcttg attgaagcta aagttgttgg catacagttc ac
//
LOCUS       DAZKLD010000119         9172 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.119, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000119 DAZKLD010000000
VERSION     DAZKLD010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9172)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9172)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9172
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.119"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            51..2828
                     /locus_tag="ACG8KR_002317"
     CDS             51..2828
                     /locus_tag="ACG8KR_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFQ0201643.1"
                     /translation="MNKLLKKEILCYLENQTAFFDLEHMNEVFTAQRLADHFGVKRNT
                     VSHYLNQLNDEQLLVKINSRPVIYFHKEAFEKQFFKLRTNFYFSIDDLKEEQPFFAQP
                     KDLFSFMIGYDASLKESIEQIKTALYYPDGGLPLLITGESGTGKSYLVNLVYQYCLLH
                     DLLEDSAPFITFNCAQYADNPELLTSNLFGHVKGAYTGAEENKKGAFEAADGGILFLD
                     EVHRLTPEGQEKLFTYLDQGVIYRMGEPNLSRRIKTRLFFATTEEITSHFLTTFIRRI
                     PIRIELPALNQRSRNERLELIYSFFLEEQRKMGSPLSVSGPALSLLTSQKLSGNIGEL
                     KNIVKVSAAKAYAEQREQPEIHVTIHHLQKELLAQPITKNTAQQGIYITQDQTLEQLM
                     EKQQPEQQRIVKSFEQILIDFKKNNCLLYSCEGKLKQEVIQLFDFLLFETSRQEKHEL
                     LVYWTQSIRETFRQMETAYQIKFDGNSVYALSYYLYQRSTIKWLPEEHDIIQLIKELE
                     QQVSQSYPSSYHYVQRILELSKIKLDIEDTSMDRILLTIYLKKAKWSKEKRLPKAMIA
                     AHGYATASSMANVVNRLLQEDLFESFDMPLDVTPQQIASEILDYCENSDVSNGLIILV
                     DMGSLKEIHQFFKKQLSVPLLILNNVTTPLAITVGECLQKNVSLEEIAEETVRQIQPE
                     WRLLYPEENKPKALITTCFTGIGTAVHLSELLEKSLPTACQLKIIPYEYQQLKDKKNS
                     DPLFSIYEVVGMIGTTDPDITNIDYLSLEDLISGEKMSVLAEWLDTTMNASEKEIFNQ
                     RIIRNFSLEKVLDSVTILDTEKVMGEIDHFMRELEVQLNRSISNPKKLALYVHVSCLI
                     ERLIRQMPIETYQGLEKWKQCQKEGLSAIKSAFSVIEEHYSVMIPNSEIAYIYDILSE
                     DTEFLSIEEEF"
     gene            2909..3328
                     /locus_tag="ACG8KR_002318"
     CDS             2909..3328
                     /locus_tag="ACG8KR_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFQ0201644.1"
                     /translation="MDREIILASHGKFASGILDSLELIYGKNHSITVFDCYTDENFDL
                     AETVRQLFMRYKDKEIIVLTDLFGGSVNNEFLQYINRDHVYLVAGLNLPLLIELVSRL
                     DAEVETTVLIQQVLTDSKKMIQFCNERLNQKIEEDEF"
     gene            3340..3810
                     /locus_tag="ACG8KR_002319"
     CDS             3340..3810
                     /locus_tag="ACG8KR_002319"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFQ0201645.1"
                     /translation="MIVLTRVDHRLLHGQVAFSWTQTIGADCILIANDDVPTNEIRKT
                     TIKLAKPQGVKLVIKSIDDSIAALKSGVTDKYKLFIVVESIEDAYKLAEAYPEIKTIN
                     LGGIKAKPGTRSIGKAVNILPEEEERLKRLIDKQIEVEIRQVPADKKVDVREVL"
     gene            3827..4606
                     /locus_tag="ACG8KR_002320"
     CDS             3827..4606
                     /locus_tag="ACG8KR_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFQ0201646.1"
                     /translation="MIVQAVLLGIVAFVAQSEYALGTSLLSRPIVTGLFTGIVLGDVK
                     TGIIMGATLELAFIGSFSVGASIPPDVVTGGILGTAFAITAGAGTETALLLGLPIATL
                     TLILKNIYLGLLIPIMNHKADNYAEEGNYKGVEHMHLLAGIGLSLMLGVVVMVSYMVG
                     SNTIGNLLNMIPDFVQKGLSVATGLIPALGFAMLARLLINKQVAPYFFLGFAIASYLE
                     IPVTGVAIFGAILAVVVVNIMNVRQLPLQTTAGEVEEDEEF"
     gene            4593..5414
                     /locus_tag="ACG8KR_002321"
     CDS             4593..5414
                     /locus_tag="ACG8KR_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFQ0201647.1"
                     /translation="MKNSEIKNSKITKKELNHVFWRSFQMEFSWNYERQMNMAYVYAM
                     IPILKKLYQSKEEMAAALKRHLEFFNTTPHIVTLILGITAAMEEENKDDPEFDVTAID
                     SIKTSLMGPLAGLGDSFFWGTLRLIATGVGTSLAMQGNILGPILFILIFNIPHILFRY
                     LATGWGYRLGTGFLKKIQANGMMESLTLGASIIGLMVVGAMTATMITINIPLKIGSGE
                     NAVTVQSIFDDIVPNILCLGAFGIVFYLLKKEVKPLTILIGLAFFGIFGSWIGIF"
     gene            5431..6480
                     /locus_tag="ACG8KR_002322"
     CDS             5431..6480
                     /locus_tag="ACG8KR_002322"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFQ0201648.1"
                     /translation="MFTMQDYIYEEKEVLSTILKKNDFSTREHMKKTVNLLILATGSS
                     YNACLAAKPALESYGDLTVDIQEPFYFFNYGKLSPSIDTVIAVSQSGKSASTVEAVKM
                     IQKQGLPVVAITNDVQSPLALEAAQIIDLGAGVESVGFVTKGYSATVLQLLLLGIGIG
                     ISKNKISKKIEQDYMQQLRKIINHLPAIIQKTEEFFDGYQSLFRLAQRFVTIGYGPNW
                     GTAKEAETKLTETIRVPSQGFELEAYMHGPYLEADASHLLFFIEGESVNKERSQKLQR
                     YMSRYVGETLTITTKKARNEKTLGLAIECDEYLSVLALVVPFQLFAYKTAVAKGIDLN
                     KKIFEDFDTVLKSKL"
     gene            6493..7497
                     /locus_tag="ACG8KR_002323"
     CDS             6493..7497
                     /locus_tag="ACG8KR_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFQ0201649.1"
                     /translation="MLKFNEEEQIKAIKGALALRPQVEEIIDKLYAEKFDAVYYLGIG
                     GTYASSMQAVTYMNGKSNLPVFVQHAAEYYTTGNKRLTKDSIVILSSVTGTTQEVVKA
                     VEQIKKVGATLIGFIDKANSKLSQLCDFVVTYPAPGTEQIKFFMAADRLMYLNGEFEA
                     YSEYYEQLERYLPTGLVEAEKKADAFGLSFAEKHRRDSMHYFIGAGNQWGAVYSYAMC
                     YWEEQSWLPSKSIHAAEFLHGTLEIVEETTPVTLFLGEDEQRVLSERVAKLLPKICSN
                     YTLIDTKDYPVEGISEKYRGRVLSFLLMHVVTQRIDAHVEQLNCHPLEIRRYYRQFDY
                     "
     gene            8130..9149
                     /locus_tag="ACG8KR_002324"
     CDS             8130..9149
                     /locus_tag="ACG8KR_002324"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046168 - glycerol-3-phosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HFQ0201650.1"
                     /translation="MKQKIAVLGPGSWGTALAQTLAENGHDVRIWGNVPEQIDEINKY
                     HTNQHFLPDLTIPESIIGYKELAEAVDNADAILFVVPTKAIRSVAQELIGKMNTKPII
                     IHASKGLEQDTHKRISEVLAEEIPEEKRQAIVVLSGPSHAEEVAVHDITTITAASIDQ
                     KAAAYVQKLFMNDYFRIYTNNDVIGVETGAALKNIIAIGAGAIHGLGFGDDAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLIVTCTSVHSRNWRAGNLLGQGHKLEEVLE
                     NMGMVVEGVATTKAAVELAQQLGVEMPITQTIYNVLYNGEDIKKAAKEIMLRDGKMEN
                     EFSLR"
BASE COUNT         3056 a         1536 c         1942 g         2638 t
ORIGIN      
        1 attaaagatt ttattgacaa aatgaatccg ttttctatga tggaggaggg gtgaataagt
       61 tgttgaagaa ggaaatttta tgttacttag agaaccagac agcctttttc gatttagaac
      121 atatgaacga ggtctttaca gcacaaaggt tagctgatca ttttggggta aaaagaaaca
      181 cagtcagtca ttatttgaac cagttaaatg atgagcaact actagtcaaa atcaattctc
      241 gaccagtgat ttatttccac aaagaagctt ttgaaaaaca attttttaag ttacgcacga
      301 atttttattt ttctatcgat gatctcaaag aggaacagcc ttttttcgcg caaccaaaag
      361 acttattctc ttttatgatc ggatacgacg ccagtttgaa agaaagtatc gaacaaatca
      421 aaacagctct ttactatcca gatggcggat taccactttt gattacaggc gaaagtggca
      481 caggtaaaag ttatttagtt aatttggtct atcagtattg tctgcttcat gatctgctag
      541 aagattctgc accattcatc acttttaatt gtgcacaata tgcagataat cctgaacttt
      601 tgactagcaa tttgtttgga catgttaagg gagcgtacac tggtgcagaa gaaaataaaa
      661 aaggggcttt tgaagcagca gatggtggga tcttgttttt agacgaggta catcgcttaa
      721 caccagaagg tcaggaaaaa ctgttcacct atcttgatca aggtgtgatt tatcgtatgg
      781 gagaacctaa tttgtcacgc cggatcaaaa cacgtctctt ttttgctacg actgaagaga
      841 ttactagtca tttcttaaca actttcattc gaagaatacc tatccgaatc gaactacctg
      901 cacttaatca gcgtagtcgt aatgaacggc tagaactgat ttactctttt tttctcgaag
      961 aacaaagaaa aatggggagc cctttgtcag tcagtggacc agctttgtca cttttaacaa
     1021 gtcagaaatt atccggaaac ataggagagc tgaaaaatat cgtcaaagtt tcagcagcaa
     1081 aagcatatgc tgaacagcgt gaacagcctg aaatccatgt cacgatccat catttgcaaa
     1141 aagaactact tgcgcagcct attactaaaa atacagcaca gcaaggaatc tatattacgc
     1201 aagaccagac cttagagcaa ttaatggaga agcaacagcc cgaacagcag cgaatcgtta
     1261 aaagttttga gcaaatccta attgatttca agaaaaataa ctgtctgcta tattcttgcg
     1321 aaggaaaatt aaagcaagaa gtgattcaat tatttgattt tttgctgttt gagaccagta
     1381 gacaagaaaa acacgagctg ctggtttatt ggacacaatc tatccgagag acctttcgac
     1441 aaatggaaac tgcctatcaa atcaaattcg atggaaacag tgtctatgcc ttgagttact
     1501 atctctatca gcgaagcact atcaaatggt taccggaaga acatgatatt atccaactaa
     1561 tcaaagaatt agaacaacaa gtttctcagt cttatccatc tagttatcac tatgtccaac
     1621 gaattctgga attaagtaag atcaaattag atattgaaga cacaagtatg gatcggattt
     1681 tactgacgat ctatctaaaa aaagcgaaat ggtcaaaaga aaaacgactg ccaaaagcga
     1741 tgattgctgc tcacggttat gcaactgcta gcagtatggc aaatgtagta aatcgtttat
     1801 tacaagagga tctatttgaa tcatttgata tgccactaga tgtcacacca cagcagattg
     1861 cttcagaaat ccttgattat tgcgaaaaca gcgatgtttc aaatggattg atcatattag
     1921 tagacatggg atctctgaaa gaaatccatc agttttttaa aaaacagtta tccgtaccgc
     1981 ttttgatttt gaataatgtg acgacacctt tagcaatcac agtaggggaa tgcctgcaaa
     2041 aaaatgtatc attagaagag atagcagaag agactgttcg acagatccag ccagaatggc
     2101 gactgctcta tccagaggaa aacaaaccca aagcactcat tactacttgc ttcactggaa
     2161 tcggtacggc ggtccactta agtgaactgc tagaaaaaag cttacctacg gcttgtcagt
     2221 tgaaaatcat cccatatgaa tatcagcaat taaaagataa gaaaaacagt gacccattat
     2281 tttccatata cgaagtcgta ggaatgattg gcaccacaga tcctgatatc accaatatcg
     2341 actatctttc attagaagac ctcatctcgg gggaaaagat gtctgtacta gctgaatggt
     2401 tggacactac tatgaatgct tcagagaagg aaatattcaa tcagcgcatc attcgtaatt
     2461 tttcattgga aaaagtactg gattccgtga ctattctaga tacagagaag gtgatgggag
     2521 aaatcgatca ttttatgaga gaattagagg tgcagttgaa tcgaagcatc agcaatccca
     2581 aaaaattagc gttgtatgtc catgtgagct gtctcattga acgattgatc cgacaaatgc
     2641 caatcgaaac ctatcaagga ttagagaaat ggaagcagtg tcaaaaagaa gggttatctg
     2701 ctattaaatc tgcgtttagt gtcattgaag agcattatag tgtcatgatc cccaattctg
     2761 agattgccta tatctatgat attttgagtg aagatacaga gtttctctcg atagaagaag
     2821 aattttgaac ataagcaagt tgttgccact aattttggca tgcaacttgc ttttttagta
     2881 tgtgtaagtg aaaagaggag atgaaaaagt ggatagagaa attatcttag cttcccatgg
     2941 aaaatttgct tcagggatat tagattcatt agagctgatc tatgggaaaa atcattctat
     3001 tactgtattc gattgctata ctgatgagaa ttttgattta gcggaaacag taaggcagct
     3061 ttttatgagg tacaaagata aagaaatcat tgttctgacg gatctttttg gcggaagtgt
     3121 caacaacgaa ttcttacaat atatcaatcg ggatcatgtc tatctcgttg ccggattgaa
     3181 tttgccacta ttgattgagc tagtatctcg gttggacgct gaagtagaga caactgtttt
     3241 gatccagcag gtgctaactg attcaaaaaa aatgatccag ttttgtaacg agcggctcaa
     3301 tcagaaaata gaagaagacg aattttagga ggaagaaaaa tgattgtatt gacaagagtc
     3361 gaccatcggt tattacacgg acaagtagct ttttcatgga cacaaacaat aggcgcagac
     3421 tgtattttga ttgctaatga tgatgtacct acgaatgaga tacgaaagac aacgatcaaa
     3481 ttagccaagc cacaaggagt caaactagtg atcaaatcaa ttgatgactc gattgccgcg
     3541 ttgaaaagcg gggtgacaga taaatataag ttattcatcg tagtggaatc gatcgaggat
     3601 gcatataagc tggcagaagc gtatccagaa atcaaaacca tcaacttagg agggatcaag
     3661 gcaaaacctg gtactcgcag catcggaaaa gcggtaaata ttttaccaga agaggaggaa
     3721 cgcttgaaac gattaattga caaacaaata gaagtagaaa tccgtcaagt accagcagat
     3781 aaaaaagtgg atgtaagaga agttttgtaa gaaaggaaga acctcaatga ttgtacaagc
     3841 agtcttatta ggaattgttg catttgtcgc acaatcagaa tatgcactag ggacatcgtt
     3901 gctttctcgt ccgattgtga cggggctgtt tacaggaatc gtgttaggtg atgtaaaaac
     3961 tgggatcatt atgggcgcaa ctttagagtt agcatttatc ggatcgttct cagtaggtgc
     4021 ttcgattcca cctgatgttg tgaccggtgg gattttaggg acggcttttg ctattacagc
     4081 tggagccggt acagaaaccg cgcttttact aggtttgccg attgctacgt tgacattgat
     4141 attgaaaaat atttacttag ggcttttgat tccaattatg aaccataaag cagacaatta
     4201 tgcagaagaa ggaaattata aaggagtcga gcatatgcac ttgcttgccg gtatcggtct
     4261 ttccttgatg cttggtgttg tggtaatggt ttcttatatg gtgggaagca atacgatcgg
     4321 aaatctatta aatatgattc ctgacttcgt tcaaaagggg ttgtcagtag ctacagggtt
     4381 gattccagct cttggttttg cgatgcttgc tcgtttgctg atcaacaaac aagttgcacc
     4441 atattttttc ttaggttttg ctatagcttc ttacttagag attcctgtga ccggagtagc
     4501 tatttttggt gcgattttag cagttgtggt agtcaatatt atgaatgtcc gtcaactacc
     4561 acttcaaaca actgcagggg aggtagaaga agatgaagaa ttctgagatc aaaaacagca
     4621 aaataacgaa aaaagagcta aatcacgtat tttggcgttc ttttcaaatg gagttttctt
     4681 ggaattatga acgtcagatg aatatggctt atgtttatgc aatgattccg attttgaaaa
     4741 agttatatca gtcgaaagaa gaaatggcag cagctttgaa acggcatcta gagtttttta
     4801 acacgacccc acatatcgtt acactgattt taggaatcac ggctgcaatg gaggaagaaa
     4861 ataaggatga tcctgaattt gatgtgactg cgatcgatag tatcaaaaca tctttgatgg
     4921 gacctcttgc agggttagga gattctttct tttggggaac tttgcgattg attgccacag
     4981 gtgtgggtac ctctttagcc atgcaaggaa atattttagg ccctatcttg ttcattttga
     5041 ttttcaatat tccgcacatt ctgtttcgct atctagcaac aggttgggga tatcgcttag
     5101 gaacgggttt tttgaaaaag atccaggcaa acggtatgat ggaaagtttg acattaggcg
     5161 cgtctattat tggactgatg gtcgttggag caatgactgc cacgatgatc accatcaata
     5221 ttcctttaaa aatcggttca ggagaaaatg ctgtgactgt tcaaagtatt tttgatgata
     5281 tcgttccaaa tatcttatgt cttggagcat ttggcatagt gttttatcta ttgaagaaag
     5341 aagtgaaacc attaacgatc ttgatcggac tagctttctt tggaattttt ggttcgtgga
     5401 tagggatttt ttaaaaaagg agcagagaaa atgtttacga tgcaagatta tatctatgaa
     5461 gaaaaggaag tactaagtac gatcttgaaa aagaatgatt tttcgacaag agaacatatg
     5521 aaaaaaacag tgaatttact gatcttagca acgggttcat cttataacgc ttgtttagca
     5581 gcgaagccag cattggaatc ttatggtgat cttactgtcg atattcaaga acctttttat
     5641 tttttcaatt atggaaaact ttctccttca atcgatactg tgattgctgt gtcacaaagc
     5701 gggaaaagtg cttctactgt tgaagcagta aaaatgatac aaaaacaagg gttacctgtt
     5761 gttgctatca caaatgacgt gcaaagtcca cttgctttag aagctgcaca aattattgat
     5821 ttaggtgccg gggtagaatc agtaggcttc gttacaaaag gctattctgc gactgtttta
     5881 cagttactac tcttagggat aggaattgga ataagtaaaa ataaaatcag caaaaaaata
     5941 gaacaagact acatgcaaca gttgcgcaaa ataatcaacc atttaccagc gattattcaa
     6001 aaaacggaag aattttttga tggatatcaa agtttatttc gtttagcaca gcgtttcgtc
     6061 accattggat atggtccaaa ctggggaaca gctaaagaag cagagacgaa attgactgaa
     6121 acaatccgcg ttccgtcaca aggatttgag ttagaagctt atatgcacgg cccatacctg
     6181 gaggcagatg cttcgcattt gctattcttt attgaagggg aaagtgtaaa taaggaacgt
     6241 tcgcaaaagt tgcaaagata tatgagtcgg tacgttggag agactctcac cattaccaca
     6301 aaaaaagcaa gaaacgagaa aacacttgga ttagcaatag aatgtgatga atatctgtct
     6361 gtattagcgc ttgtcgttcc gtttcaacta ttcgcttata aaaccgcagt agctaaaggt
     6421 attgacttga acaaaaagat ttttgaagac tttgatacag tcttgaaaag taaattataa
     6481 gaggtggaaa tcatgctgaa attcaatgaa gaagaacaaa tcaaagcaat aaaaggcgca
     6541 ctagctttac gtccgcaagt agaagaaatc atcgataaac tttatgcgga aaaatttgac
     6601 gctgtctatt atctaggaat cggtggaacg tatgcttcct cgatgcaagc tgtcacttat
     6661 atgaatggga aaagcaactt gcctgtcttt gtacagcatg ctgcagaata ttacactact
     6721 ggaaacaaaa gattaacaaa agattctatc gttattttgt cctctgttac cggtacaact
     6781 caagaagtag tgaaagcagt agaacaaatc aagaaagtag gagcgacact gattggattt
     6841 atcgataaag cgaacagtaa gttgtctcag ctatgcgatt ttgtggtcac ttatccagct
     6901 cctggaacag aacaaatcaa attttttatg gcagcagatc gattgatgta tttgaatgga
     6961 gaattcgaag cttattcaga atactatgaa cagctcgaac gttatttacc aacagggtta
     7021 gtggaagcag agaaaaaagc agatgcattt ggcttatcat ttgcagaaaa acatcgacgt
     7081 gatagtatgc attattttat tggagcaggg aatcaatggg gagctgttta ttcatatgcc
     7141 atgtgttact gggaagaaca aagctggctt ccatcgaaat ctatccatgc tgccgagttt
     7201 cttcacggga cgttagagat cgtagaagaa acaaccccag tcacattatt tttaggcgaa
     7261 gatgaacaga gagtgttatc tgaacgtgtg gctaaacttt tgccaaaaat ttgcagcaat
     7321 tatacattga tagacacaaa agactatcca gtagaaggta tcagtgaaaa atatcgcgga
     7381 cgtgttttgt ctttcttatt gatgcatgta gtgacacaac gcatcgatgc tcatgtggag
     7441 cagttgaact gccatccatt agagatacgc cgctactatc gtcagtttga ttattaggat
     7501 agactggata aaaagaatat aaaaaataaa gagagcagcg ataactgctc cctttatttt
     7561 tttaaaatat tggcatcaat tacgttataa aacacatttt ttgcattatc tacagttcaa
     7621 cttataatct gtttaatggg acatgtgggt gccaaccatt ttttgttatg caatagtact
     7681 atttagttgt tgcatggatc cgtagtaaaa gtccaagtac aacgagttgc gccagtttac
     7741 atagcgtttt gtatccagaa tgttgactaa aacaaaagtt cctatgtcaa ccgtcaacag
     7801 agacaatctg tccgttgcag gacctgacag atttagaaat gttcaatctg tctcttatgt
     7861 attttaaatc aatactcact aggagaagtt tcctaacctc ctaatagtaa tgctggtagt
     7921 aacattgtca aaactgcttc ctcttaggaa caagcaaagc ataacgagtg taaaaataaa
     7981 atgtagacta ttgtaacata tcattgatta tttcaagtaa aaacgataaa agaagtaaac
     8041 aaacacttaa aaacaatgga aattagtccc aactttaaga aatgtatact ataatggaaa
     8101 ttatcaatta tttatttgga ggaatatcca tgaaacaaaa aatcgcagtt cttggtcctg
     8161 gatcttgggg cactgcttta gcacaaacat tggcagaaaa cgggcatgat gtccgtattt
     8221 ggggaaatgt acctgaacag attgatgaaa tcaataagta tcatacaaat cagcattttt
     8281 tacctgattt gacgatccca gaatcgatta tcggatataa agaattggca gaagcagtgg
     8341 ataacgcaga tgctatttta tttgttgttc caacaaaagc gattcgttca gtcgcacagg
     8401 aacttattgg taaaatgaat actaagccaa tcattataca tgcaagtaaa ggactagaac
     8461 aagatacgca taaacgaatc tcagaagttt tggctgaaga aattccagaa gaaaaaagac
     8521 aggcaattgt tgtattatct ggtcctagcc atgctgagga agtggcagta catgatatca
     8581 caacaattac agctgcaagt attgatcaaa aagcagcagc ctatgtacaa aaactgttca
     8641 tgaatgatta tttcaggatt tatacaaata atgatgtgat tggcgtagaa actggtgcag
     8701 cactgaaaaa tatcattgca attggtgccg gcgctatcca tggattagga ttcggagatg
     8761 atgcaaaagc agcaatcatg actcgaggat tggcagaaat cagccgtctt ggtgtcgcga
     8821 tgggtgccaa tcctctgaca tttatcggtt tgagtggtgt gggggacttg atcgttactt
     8881 gtacaagtgt tcattctcgc aattggcgtg ccggtaactt gttaggacag ggacataagc
     8941 tggaagaagt tctagaaaac atgggaatgg tcgttgaggg tgtggcaacg acaaaagcag
     9001 cagtagagct agctcaacag ctaggtgttg agatgccaat tactcaaaca atctataatg
     9061 ttttgtataa tggagaagat atcaaaaaag cagcaaaaga aatcatgtta cgtgacggaa
     9121 aaatggagaa tgaattttct cttcgttaaa tagatgatca atagttaatc gt
//
LOCUS       DAZKLD010000120         8952 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.120, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000120 DAZKLD010000000
VERSION     DAZKLD010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8952)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8952)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8952
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.120"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(7..1812)
                     /gene="glmS"
                     /locus_tag="ACG8KR_002325"
     CDS             complement(7..1812)
                     /gene="glmS"
                     /locus_tag="ACG8KR_002325"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:1901137 - carbohydrate derivative
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HFQ0201651.1"
                     /translation="MCGIVGMIGLKNVTPGLIDGLEKLEYRGYDSAGIFVSDGTTDYL
                     VKAQGRIQNLKNKINIDTTGNIGIGHTRWATHGQPSEENAHPHTSQSGRFVLVHNGVI
                     ENFEELKIAYLANDHFIGETDTEIIAHLIETFAKDTTTQEAFLKALRVIKGSYAFALI
                     DRTAPDVIYVAKNKSPLLIGLGDGFNVIASDAMAMLAHTKEFVEIEDEEMVTVTSEKV
                     IIQNFAGDIVERSSFEAQVDSSDIEKGTYPFYMLKEIDEQPIIMRRIAQKYVTEDNRV
                     SLDQKLVDTLSDSDRIYIVACGTSYHAGLAGKQTLEKLTQIPVEVHLASEFGYNTPLL
                     SKKPFFIFLSQSGETADSRQVLVKINRLGYPSLTITNVAGSTLSREASFTLLLHAGPE
                     IAVASTKAYTAQIAVLTLLAKAIGDKKEFETSLVFDITHELSLVANAMESVIAQKDYL
                     EELAEVYLSDTRNAFYIGRGADYNVSLEAALKLKEISYIQAEGFAAGELKHGTIALIE
                     EGTPVIGIISEEVTGAHTRGNLKEVESRGAKTLVIVSEGLEKESDQLVLPTVHPYLTT
                     LTTVVPTQLLAYYATLLRGFDVDKPRNLAKSVTVE"
     gene            2222..2512
                     /locus_tag="ACG8KR_002326"
     CDS             2222..2512
                     /locus_tag="ACG8KR_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0201652.1"
                     /translation="MDSKIEIMTLGMLKKQLSKFEASAGVSDDTKIFLDTGWDSIQEI
                     APDALEVAEAREFTVEDELTKESFSGYAREEKAERFDTSEQSETVIVIKNLY"
     gene            2525..3550
                     /gene="sppA"
                     /locus_tag="ACG8KR_002327"
     CDS             2525..3550
                     /gene="sppA"
                     /locus_tag="ACG8KR_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HFQ0201653.1"
                     /translation="MNKRRWAAVLIAAGLFVVSLVSASLTKPQENDQIEGLNSWLYGD
                     EELASNVLEEGDSTQKIVKLSVDGTIAAGGASGLFASEGYNHKNFMEQLKAIEEDPTV
                     KGIFLEVNSPGGGVYESAEIAKKLDTIRKEHDIPMYVSMKNMAASGGYYISAQADKIF
                     ATEETVTGSIGVIMSGLNYSGLLEKLGVEDTTVKSGALKDMGSGTRPETKEEEAVLQA
                     YIDSAYSRFVAIVSEGRNKSEEEVKKIADGRIYDGVQAKEVGLVDELGFPEDALKAMR
                     KEQQLEDAELVEYSSTPSGFASTWLGSKLAEFQGLKPSETSQILSVLENLGTAESPKA
                     MYYYGGE"
     gene            3553..4212
                     /locus_tag="ACG8KR_002328"
     CDS             3553..4212
                     /locus_tag="ACG8KR_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HFQ0201654.1"
                     /translation="MSELSNENKEQEKDKQQQPAVGPDSNFSQKVLSSFEREPLTPEE
                     IKKKQRKWKKYTDTRNEPPKANDFPKYFYAGFFIRMFAFLVDLLCIGAITRLTLGIAK
                     NLGWLNFSDSYLSIYGFTALLIYLAYFILLTKFNHGQTIGKMIFGIRVISFDEEELSW
                     STVLVREGACRFILKFPLLMLGYLPAIFNQKKQHLGDFFTNTSVVTINLIKAFNQEVN
                     S"
     gene            4369..5301
                     /locus_tag="ACG8KR_002329"
     CDS             4369..5301
                     /locus_tag="ACG8KR_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0201655.1"
                     /translation="MKPIDFPEKYENLMRVAQQALANQQYQQAKELFQRAYELKESFE
                     ANSLLVFCLYELDEKKEALKQALLHEKQYLQNEEFAEFYFDLLISTQDFLYARKLIAS
                     TDFYEAFEQRVIEKIQFAEELSGQMERQKIKALHNKSEELPSLEPARQLASIDEIEQL
                     PYHEFIQTASKLIVSPEVHILARAKLLETLRQLNECHPVFYLTIEEKLVKVIPKDLPK
                     PQQQASYQQLCVFSDRYGNEDALLSSVLNEEFTLQSAIVYPVYDTYIEDPKRWFQLTV
                     EACTGKTMYEGTEDEKQDFLKKREKILQQMIFFH"
     gene            5455..6744
                     /gene="tig"
                     /locus_tag="ACG8KR_002330"
     CDS             5455..6744
                     /gene="tig"
                     /locus_tag="ACG8KR_002330"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HFQ0201656.1"
                     /translation="MTATFEKKGTNDGVLKFTIAQDEIQKGLTTAFNKVKKSLNVPGF
                     RKGKVSRQVFNRMYGEEALYEDALNAVLPEAYEAAVKEAGIDPVSQPKIDVESMEKGQ
                     DWVITAEVTVKPEVKLGQYKDLVVEKQDREVTDADVDARIKREQEAQAELVIKEDAAA
                     ENGDTVVIDFEGFVDGEAFDGGKGENYSLELGSGSFIPGFEDQLVGHKSGESLDVNVT
                     FPEDYQAEDLAGKEAVFKVTIHEVKAKEVPELDDEFAKDVDDEVSTLDELKEKYRKEL
                     VETKEKAADDAKDEAAIRMAVENAEIVELPHVMVHDEVHRSMDEFLNNMQRQGISPEM
                     YYQLTGSTEEDLHKQFEGEAEMRTRTNLVIEAIAAAENLQATEEEINAEIQELSETYN
                     MPIEQIKRVLTEDMLEHDVTMKKAIELITGTATEAKE"
     gene            6991..8241
                     /gene="clpX"
                     /locus_tag="ACG8KR_002331"
     CDS             6991..8241
                     /gene="clpX"
                     /locus_tag="ACG8KR_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HFQ0201657.1"
                     /translation="MYDNPSSNETVRCSFCGKTQEEVKKIVAGPGVYICNECIDLCKE
                     IIDEEFYEEAVREFTEVPKPLEILEVLNNYVIGQDRAKRSLAVAVYNHYKRVNQQAQE
                     NTDDVELQKSNICLIGPTGSGKTFLAQTLAKTLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLLQSADYNVERAEKGIIYIDEIDKIARKSENVSITRDVSGEGVQQALLKILEGTV
                     ASVPPQGGRKHPHQEFIQIDTTNILFIVGGAFDGIETIVKNRMGEKTIGFGTQNQKLS
                     EDESVMQHIIPEDLLKFGLIPEFIGRLPVMAALEKLTTDDLVRILTEPKNALVKQYQK
                     LLSLDNTELEFEPEALRAIAKKAIERNTGARGLRSIIEEIMMDVMFDIPSNENIEKVI
                     ITKEAAELSGEPTVIYKDSEKKAG"
     gene            8324..8911
                     /gene="yihA"
                     /locus_tag="ACG8KR_002332"
     CDS             8324..8911
                     /gene="yihA"
                     /locus_tag="ACG8KR_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HFQ0201658.1"
                     /translation="MKVHQAEIIISAVAPKQYPDTQLPEIALAGRSNVGKSSFINTLI
                     DRKNLARTSGKPGKTQTLNFYLIENALHFVDVPGYGYAKVSKTERAKWGKMIETYLTT
                     RDQLRAVVSLVDLRHKPSQEDIQMYQFLKYYDIPVIVVATKADKIPRGKWNKHESEIK
                     KALDFDRNDTFILFSSVTKQGKEEAWAAIEQAIES"
BASE COUNT         2983 a         1558 c         1897 g         2514 t
ORIGIN      
        1 cttttattat tctactgtca ctgatttcgc taaattacgt ggtttgtcaa cgtcgaatcc
       61 ccgtaatagc gttgcgtaat atgcaagtaa ttgagtcggt acgactgtgg ttagagtagt
      121 tagataaggg tggacagttg gtagcaccag ctggtcactt tccttttcta atccctctga
      181 gacaataact agtgttttag cgccacggct ttcgacttct ttcaggtttc ctcgagtatg
      241 cgcaccagtc acttcttcac tgatgattcc aatcactggt gttccttctt cgatcaaagc
      301 aatcgttcca tgtttcaatt cgccagcggc aaaaccttct gcctggatat aactgatttc
      361 tttcaatttc aaagcagctt caagagaaac gttgtagtca gcgccacgtc caatgtaaaa
      421 ggcatttctt gtatcgctga gatatacttc tgccaactct tctaaataat ctttttgggc
      481 gatgaccgat tccatcgcat tagcaactaa actcaattca tgagtgatat cgaatacaag
      541 agaagtttca aattcttttt tatcaccgat agctttggct aataaagtta gtacagctat
      601 ttgtgcagta taagctttcg ttgatgctac tgcgatttcg ggtccagcat gcaatagcag
      661 tgtaaaggat gcttcccgcg aaagagtaga accagcaaca tttgtaatcg taagtgaagg
      721 gtagccgaga cggttgattt ttaccagaac ttggcggcta tctgctgttt cgccactttg
      781 actcaagaaa ataaagaatg gtttttttga aagaagaggt gtgttatagc caaattcact
      841 ggctaaatga acctcaacag ggatctgtgt caatttttcc agagtctgtt tacctgctaa
      901 ccccgcatgg taactagttc cgcaagcaac gatatagatt cgatcgctat cagacaatgt
      961 atcgaccagc ttttgatcaa gagagacacg attgtcttct gttacgtatt tttgagcgat
     1021 acgacgcatg ataataggct gttcgtcgat ttctttcagc atataaaaag gataggtacc
     1081 tttctcgata tctgaagaat ctacttgtgc ttcgaatgaa gaacgttcta cgatgtcacc
     1141 tgcaaaattt tgaataataa ctttttctga tgtgacggta accatctctt catcttcaat
     1201 ctcaacgaat tccttcgtat gagcaagcat agccattgca tcgctggcaa tcacattgaa
     1261 tccatcgccg agtccgatta atagtggact tttattttta gcgacgtaaa taacatcagg
     1321 tgctgtccga tcaatcaaag cgaaagcgta agaacctttg atgacccgca aagcttttaa
     1381 aaacgcctct tgcgttgtag tatccttcgc gaaagtttca attagatgag cgataatctc
     1441 agtatcggtc tcaccaataa agtggtcatt tgctaaataa gcaattttaa gttcttcaaa
     1501 gttttcgatt acgccattgt gaacgagcac gaagcgtcca ctttgggaag tatgtggatg
     1561 ggcgttttct tcagaaggtt gcccgtgagt tgcccagcgt gtatgaccaa tacctatatt
     1621 tcctgtagta tcaatattta ttttgttttt caaattttga attctgcctt gtgctttgac
     1681 cagataatca gttgtgccat ctgagacaaa gataccagct gaatcataac cacggtattc
     1741 taatttttct agaccgtcaa tcaatcctgg tgttacattt tttaatccaa tcattccaac
     1801 aattccgcac ataaaaaaca tcctactttc tataatgaat tcgtatgaat gcttccagct
     1861 gcaggaaaat ccttttgttt tatctttgca gatatttttt gtggattttc tgtagagtgc
     1921 agtccctagg gccatccgcc gaatctttcg ataaaccctc tcctcgtcat ctgtttacac
     1981 agacctggcg ctatcctaaa aggtataact atcctcctct tttgtttttt atctggaaac
     2041 atttaaaatc ataacctttt gcgaatgttc ttgtcaacaa aaaaagaata aaaaataaat
     2101 tggtatatat caaatgcttt gtcaaatgct ctttttatgt caaaaaataa tgggttttta
     2161 tctatcatct ttttcaggag ttcggtacaa tagaaagtaa tcggatagag gaggagtaaa
     2221 aatggattca aaaattgaga tcatgacact gggaatgtta aaaaaacaat tgtccaaatt
     2281 cgaagcatca gctggtgtat cagatgatac gaaaatattt ttagatactg gctgggatag
     2341 catccaagaa atcgcacctg atgccttgga agttgctgaa gctcgtgagt ttacggtaga
     2401 agatgaattg acaaaagagt cgttttccgg gtatgcgcgg gaagaaaaag cagagcggtt
     2461 cgacacttct gagcagtcag aaactgtgat cgtcataaaa aacttgtatt agaaaggaga
     2521 aagaatgaat aagagacgtt gggcagcagt tctgattgct gcaggattat ttgtggtttc
     2581 gttagtttca gctagtttga ctaagcctca ggaaaacgat caaattgagg gattgaacag
     2641 ttggttatat ggggatgaag aactagcttc aaatgtgttg gaagagggag acagtacgca
     2701 aaaaatcgta aaattatcag tagatggaac gattgctgcg ggaggggctt caggtttatt
     2761 tgcttctgaa ggttacaatc ataaaaattt tatggaacaa ctgaaggcga ttgaagaaga
     2821 tccaacagtc aaaggtatct ttttagaagt aaattctcct ggtggcggtg tctacgaaag
     2881 tgcggaaatc gctaagaaat tggatacgat tcgtaaagaa catgatatcc caatgtatgt
     2941 cagcatgaaa aacatggcag ctagcggtgg ctactatatt tctgctcaag cagataaaat
     3001 ctttgcaaca gaagaaacag tgaccggctc aattggtgtg atcatgtcag gattgaatta
     3061 ttcaggactt ctagaaaaac tgggtgtgga agatacaaca gttaaaagcg gagcgttgaa
     3121 agatatgggt tcaggaacgc gaccagagac aaaagaagaa gaagcggtgc ttcaagcata
     3181 tatcgacagc gcatatagtc gttttgtagc aatcgtcagc gagggacgga ataaatctga
     3241 agaagaagtc aaaaaaattg ctgatggaag gatttatgat ggtgtccaag caaaagaagt
     3301 aggtttagtc gatgaattgg gatttcctga agatgcttta aaagctatgc gaaaggaaca
     3361 acagcttgaa gatgcggaac tagtagaata ttcttccacg ccatctgggt ttgctagtac
     3421 atggttagga agtaaactag cagaattcca gggattgaag ccttctgaga ctagtcagat
     3481 cttatccgtt cttgagaatc tagggacagc ggaatcacca aaagcaatgt attattacgg
     3541 aggtgagtaa gcatgtcaga gctttcaaat gaaaataagg aacaagaaaa agataagcaa
     3601 cagcagcctg ctgtaggacc tgactccaac ttttcccaaa aggtactgag ctcttttgaa
     3661 agagagcctt tgacgcccga agaaatcaaa aagaaacaac gtaaatggaa aaagtatacg
     3721 gacacaagaa atgaaccgcc aaaagcaaat gattttccta agtatttcta tgcaggtttt
     3781 tttatccgaa tgtttgcttt cttggtcgat cttttgtgta tcggagctat tacgcgttta
     3841 acacttggta ttgccaaaaa tctaggttgg cttaattttt cagattctta tctgagtatc
     3901 tacggattca cagccttact tatttatcta gcgtatttta ttttattgac gaaattcaat
     3961 catggtcaaa cgattgggaa aatgatcttt ggtattcgag tcatcagctt tgatgaagaa
     4021 gaacttagct ggtcaaccgt tttagttaga gaaggtgctt gccggttcat tctaaaattt
     4081 cctttgctga tgttagggta tcttccagca atctttaatc agaaaaagca acatctaggc
     4141 gattttttca ctaatacaag tgttgttacg atcaatctga tcaaagcatt caaccaggaa
     4201 gtcaacagct gatgagcaat aaaaagcagt aacagttgaa agcaccttct aaaagtcaga
     4261 tctaaaatct agcttttaga gggtgcttca gttattttta attgtcatgc tttttttgtt
     4321 ttgctactat tgaacatgag ctgaacatga gcaagaggag gacactgcgt gaagccgatt
     4381 gattttccag aaaaatatga gaatttaatg cgagtagcac aacaagcact agcaaaccag
     4441 caatatcagc aggcaaaaga attatttcaa cgagcttacg agttgaaaga aagctttgag
     4501 gctaatagcc tgcttgtttt ttgtctttac gagttggacg agaaaaaaga agcattgaag
     4561 caagcactct tgcatgaaaa acagtatctt caaaatgagg agtttgctga attttatttt
     4621 gacttgttaa tcagtacaca agatttttta tatgctagaa aattgatagc aagtacagat
     4681 ttttatgaag ctttcgagca gcgggtcatt gaaaaaatcc aatttgcaga agaattatcc
     4741 ggacaaatgg agaggcagaa gatcaaggca ctccacaata aatcggagga acttccttcg
     4801 ttggaacctg cacgtcagct tgcgtctatt gatgagatcg aacagcttcc ttatcatgag
     4861 tttatccaaa cagcgagtaa acttattgta tcgccagagg tccatatttt agcaagggca
     4921 aaattactgg agacacttag gcaattgaac gaatgccatc ctgttttcta tttgacgatt
     4981 gaagaaaagt tagtcaaagt cataccaaaa gatctgccaa agccgcagca gcaggcaagt
     5041 taccagcaat tatgtgtatt ttcagatcgt tacggtaatg aagatgcgct gcttagttcc
     5101 gtgttaaatg aagaattcac gttgcaatct gcaattgttt atcctgtcta tgatacatac
     5161 atcgaagacc cgaagcgctg gtttcaactg actgtagagg cgtgtacagg aaaaacgatg
     5221 tatgaaggta cagaagatga aaaacaagat tttttaaaaa aaagagaaaa aattttgcag
     5281 caaatgatct tcttccatta gattttatca gtgaaaaaga taaaaatgtt atgaaaaaca
     5341 aggattctat ccattcaata tgtttacaat ttgaaagggt tagtgtacta tttaaaggta
     5401 tgcatgaaac gtttttgatg tataaaatga atttctaaac tcggagggaa ttaaatgact
     5461 gcaacatttg aaaaaaaagg cacgaatgat ggtgttttaa aatttacgat tgcgcaagac
     5521 gaaatccaaa aaggattaac aactgcgttt aataaagtga aaaaaagctt gaacgtacct
     5581 ggattccgta aagggaaagt ttctcgtcaa gtattcaacc gtatgtacgg tgaagaagct
     5641 ttatatgaag atgcattgaa tgctgtatta ccagaagctt atgaagctgc tgtcaaagaa
     5701 gctggcatcg atcctgtttc tcaaccaaaa atcgatgttg aaagcatgga aaaaggtcaa
     5761 gactgggtaa tcactgctga agtaacagtg aaaccagaag taaaattagg acaatataaa
     5821 gatctagttg ttgaaaaaca agaccgtgaa gtaacagatg ctgacgtgga tgcacgtatc
     5881 aaacgtgaac aagaagcaca agctgaatta gttatcaaag aagatgctgc agctgaaaat
     5941 ggcgatacag tagtaatcga cttcgaaggc tttgtagatg gcgaagcgtt tgatggcgga
     6001 aaaggcgaaa attactcact tgaattaggt tcaggctcat tcattccagg ttttgaagat
     6061 caattagttg gtcataaatc aggtgaaagt ctagatgtaa atgtaacttt cccagaagat
     6121 tatcaagcag aagatcttgc tggaaaagaa gcagtcttca aagtgacaat ccatgaagta
     6181 aaagcaaaag aagtgcctga attagatgac gaattcgcaa aagatgttga tgacgaagta
     6241 tcaacattag acgaattgaa agaaaaatat cgcaaagaat tagtagaaac aaaagaaaaa
     6301 gcagcggacg atgcaaaaga tgaagcagcg atccgtatgg ctgttgaaaa cgcagaaatc
     6361 gttgaattgc cacacgtgat ggttcatgat gaagtacatc gttcaatgga cgaattcttg
     6421 aacaacatgc aacgtcaagg tatctctcct gaaatgtact atcaattaac tggttctaca
     6481 gaagaagact tgcataaaca attcgaaggt gaagcagaaa tgcgcacaag aacaaatctt
     6541 gtgatcgaag caattgcagc tgctgaaaat cttcaagcaa cagaagaaga aatcaatgca
     6601 gaaatccaag aattatcaga aacatacaac atgccaatcg aacaaatcaa acgcgtgttg
     6661 acagaagata tgctggaaca tgatgtgaca atgaaaaaag caatcgaatt gatcacaggt
     6721 acagcgacag aagcgaaaga atagaaatca agtaataaat catttctgaa gaagctactt
     6781 ctagaaaatt gtttattcga aataagggac tgtgacacga ttttgtcaca gttccttatt
     6841 ttttgttaag agtaaaaaat gcaaaggtat aggtcagttg atcctttgtt aaacactttt
     6901 tctttttact aaactatgtt atgatagtaa aagactgtaa agtctttgat agtttcttgc
     6961 atactttttg caagttaagg ggtgaagacc atgtatgaca atccaagtag caatgagacc
     7021 gtacgctgtt ctttttgcgg gaaaacccaa gaagaagtga aaaagatcgt agctggtcca
     7081 ggggtctaca tctgtaatga atgtatcgac ctatgtaaag aaatcattga tgaagaattt
     7141 tatgaagaag ctgtacgcga attcactgaa gtccctaaac cactagaaat tcttgaagta
     7201 ttgaataact acgtgattgg acaagatcgc gcaaaacgct cgttagctgt tgccgtatac
     7261 aatcactata aacgtgtcaa tcagcaagca caagaaaaca cagatgatgt tgaactgcaa
     7321 aaaagcaata tctgcttgat cggacctaca gggtctggta aaacattttt agcacaaaca
     7381 ttagcaaaaa cattgaatgt accttttgca atcgcagatg cgacaagttt gacagaagct
     7441 ggttatgtgg gggaagatgt agaaaacatt ttgcttaaat tgcttcaatc tgctgattac
     7501 aatgtggaac gtgcagaaaa agggatcatc tatattgatg agatcgataa aattgcgcgt
     7561 aaaagcgaaa atgtatctat tactcgtgac gtttccggcg aaggtgtcca acaagccttg
     7621 ctgaaaattc tagaaggaac tgttgcaagc gtgccacctc agggaggacg taaacatcca
     7681 catcaagaat ttattcagat tgatacgacg aacatattgt tcatcgtagg tggtgcattt
     7741 gatggcatcg aaacgattgt gaagaatcgt atgggcgaaa agacgattgg atttggtaca
     7801 caaaaccaaa aactttctga agacgaaagt gtcatgcagc atatcattcc tgaagattta
     7861 ctaaaattcg gattgatccc tgagtttatc ggacgattac ctgttatggc agctttagag
     7921 aaattgacaa cagatgatct ggtacggatc ttgactgagc cgaaaaatgc gctagtcaaa
     7981 caatatcaaa aacttctttc tctagataat accgaacttg aattcgaacc tgaagcgttg
     8041 agagccattg cgaaaaaagc aatcgaacgc aataccggtg ctcgaggact tcgttccatc
     8101 attgaagaaa tcatgatgga tgtgatgttt gatatccctt caaatgaaaa catcgaaaaa
     8161 gtgattatca caaaagaagc agctgaattg tctggtgagc caacagtaat ctataaagat
     8221 tcagagaaaa aagcgggttg atgaaaagag gctgccgtaa aagtcatttt ggcttttgcg
     8281 acagccgctt gctttattca tcaccttgtt aaggagagag accatgaaag tccatcaagc
     8341 tgaaattatc ataagtgcag tggcaccaaa acaatatcca gatacacaat tgccagaaat
     8401 agctctggca ggacgatcaa atgtagggaa atcttctttt atcaacacat tgattgatcg
     8461 aaagaattta gcccgtactt ccggtaagcc aggaaagacc cagacattga atttttattt
     8521 gatcgagaac gcattacatt ttgtcgatgt tccgggatat gggtatgcaa aagtatccaa
     8581 aactgagcga gcaaaatggg gaaagatgat cgagacatat ttaacgacta gagaccagct
     8641 acgtgcggtc gtttctttag tagatttgcg tcataagcct tctcaagaag atatccagat
     8701 gtatcaattt ttgaagtatt atgatatccc tgtaattgtt gttgccacta aggcagataa
     8761 gatccctcgt ggtaaatgga acaagcatga gagcgaaatc aaaaaagcgt tggattttga
     8821 tcgcaatgac acgtttattc tcttttcttc cgttacaaaa cagggaaaag aagaagcgtg
     8881 ggcagcaatc gaacaagcaa ttgaatcata aaaagaataa aaaagactta ccagtcaaac
     8941 tctttcataa aa
//
LOCUS       DAZKLD010000121         8851 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.121, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000121 DAZKLD010000000
VERSION     DAZKLD010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8851)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8851)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8851
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.121"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(12..482)
                     /locus_tag="ACG8KR_002333"
     CDS             complement(12..482)
                     /locus_tag="ACG8KR_002333"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004602 - glutathione peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HFQ0201659.1"
                     /translation="MSIYNFETTLEDGTVYSLKKYAGKPIIIVNTATKCGLAPQFKQL
                     EELYQAYQTQGLVILGFPSNQFKQELDSATEAAEACKTTYGVTFPMHQLIDVNGENTD
                     PLFTYLKNEAPGTVTKAIKWNFTKFLIDRNGKVIERFAPQTSPLKMTAAIESVL"
     gene            complement(641..1099)
                     /locus_tag="ACG8KR_002334"
     CDS             complement(641..1099)
                     /locus_tag="ACG8KR_002334"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0043768 - S-ribosylhomocysteine lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HFQ0201660.1"
                     /translation="MRMNVESFNLDHTKVKAPYVRVADRKTGAHGDVIIKYDVRFKQP
                     NKEHMDMPSLHSLEHLTAELIRNHADYIVDWSPMGCQTGFYLTVLNHESYEDILDVLE
                     ATMKDVLEATEVPASNETQCGWAASHTLEGAQALAREFLAKRDEWSEVEA"
     gene            complement(1270..2415)
                     /locus_tag="ACG8KR_002335"
     CDS             complement(1270..2415)
                     /locus_tag="ACG8KR_002335"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HFQ0201661.1"
                     /translation="MHIQTKLIHGGISEDPTTGAVSVPIYQTSTYRQDGVGQPKQYEY
                     SRSGNPTRFALEELIADLEGGVRGFAFSSGLSGIHAVFSLFQAGDHILLGDDVYGGTF
                     RLFDKVLTKNGLEYTIIDTSNLDKIEQSIKPNTKALYLETPSNPLLKITDLEKSATLA
                     HQHGLIVIADNTFATPYFQRPLDLGSDIVVHSGTKYLGGHSDVVAGLVTSNHKDLADQ
                     IGFYQNAIGAVLGPQDSWLLQRGIKTLSVRMEEHQKNAFVVADFLFSHPAVEKVYYPG
                     LPDHELHGVAKQQMSGFSGMISFTLKNEESAIPFVESLQLFTLAESLGGVESLVEIPS
                     VMTHASIPKEKREEAGIKDGLIRLSVGIEYGQDLINDLAQAFDRIKN"
     gene            complement(2428..3345)
                     /locus_tag="ACG8KR_002336"
     CDS             complement(2428..3345)
                     /locus_tag="ACG8KR_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent cysteine synthase family protein"
                     /protein_id="HFQ0201662.1"
                     /translation="MIISTITEAIGSTPLYRFDNQHYSMPKGSAIYAKLEYLNPGGSI
                     KDRLGSYLIKEGFAQGKINEKTTIIEPTAGNTGIGIALAALTYKLRTVFVVPEKFSLE
                     KQQLMKALGAKIIHSPSEQGIIGAISKSKKLAEEISNSYLPLQFENKDNPAAYYHTLG
                     PEIFHEIKENIHSFVAGIGSGGTFAGTSTFLKEKYPDIRIIGVEPEGSVLNGGDPAPH
                     EIEGIGVEFIPPFLSPLSINQIETISDVEGFNYTRQLAREQGLLVGSSSGAAFAAALR
                     EIRRLPPGHRVVTIFPDAADRYLSKNIYL"
     gene            3575..4894
                     /locus_tag="ACG8KR_002337"
     CDS             3575..4894
                     /locus_tag="ACG8KR_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380-like element IS1678 family transposase"
                     /protein_id="HFQ0201663.1"
                     /translation="MTSLHKNQVKFNSNITISHTGGQLSSDSGLVLVKELMNIFGFSE
                     LAKQHIHIEDERAYFTHDNLSILEQFIMQLIAGYSADSAANLLRQDPVFKAVLDRKEL
                     ASQSSLSRFLDRLSEENIHELQALNQELIDKARLIRNDTELIIDLDSTHSDTFGHQEQ
                     TNYNTHYQTYGYHPLVAFDGLTGDFLKAELRSGNQYTSKGVKEFLTPLLEHYNHSLPN
                     TDILVRGDSGFATPGVYDSCESKKSHYVIRLKNNRRLGQIAEKSVLYGDNQKWEEREV
                     QYFSTTYQAQSWSQSRRVCIRSTREAGELLFRHEFIVTNLSENVSPDTIFSIYAKRGT
                     MENFIKEAKAGFYFDKTDSPRFLENHVRMMLSLIAYNLVNFLRTIGFEEVQKGMTIHS
                     IRLKFLKVAGKLVQTGRRVYLKLSSYHVYQNEFYRVFARLRRASQWI"
     gene            5098..5340
                     /locus_tag="ACG8KR_002338"
                     /pseudo
     CDS             5098..5340
                     /locus_tag="ACG8KR_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455809.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(5505..6119)
                     /locus_tag="ACG8KR_002339"
     CDS             complement(5505..6119)
                     /locus_tag="ACG8KR_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFQ0201664.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDYVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVKLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSADAKDPQ
                     RRLVYKSIVEDLKNGVAIAKIAKDYNVTRQTVYRIKKDSMVNDK"
     gene            6569..7894
                     /locus_tag="ACG8KR_002340"
     CDS             6569..7894
                     /locus_tag="ACG8KR_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HFQ0201665.1"
                     /translation="MMDYSKEPVNDYFLIDMKSFYASVECIERNLDPLTTELVVMSRA
                     DNTGSGLILASSPEAKKRYGITNVSRPRDLPQPFPKTLHVVPPRMNLYIKRNMQVNNI
                     FRRYVADEDLLIYSIDESILKVTRSLNLFTTEGTRSQRRKKLAQMIQENIKEELGLIA
                     TVGVGDNPLLAKLALDNEAKHNEGFVAEWTYENVPEKVWNIPEMTDFWGIGSRMKKRL
                     NQMGIMSIRDLANWNPYTIKNRLGVIGLQLYFHANGIDRTDIAIPPEPTKEKSYGNSQ
                     VLPRDYTRRNEIELVVKEMAEQVAIRIRQHNCKTGCVHLHIGTSILETRPGFSHQMKI
                     PITDNTKELQNYCLFLFDKYYEGQEVRHVGITYSKLVYTDSLQLDLFSDPQKQIDEEN
                     LDKIIDKIRQKYGFTSIVHASSMLEGARSITRSTLVGGHAGGNGGIKND"
     gene            7887..8237
                     /locus_tag="ACG8KR_002341"
     CDS             7887..8237
                     /locus_tag="ACG8KR_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0201666.1"
                     /translation="MIDYNDRGRLKWAGFYLSDHTEEIDADNEQRTSQNLAKEQMTTE
                     EIAEVLNAAVIKNRSVSIQKEERNAEGYYPEDIIGKILGSDELGIYVGKDKIHYDEIR
                     HIEFYQDNKWSRLE"
     gene            8234..8413
                     /locus_tag="ACG8KR_002342"
     CDS             8234..8413
                     /locus_tag="ACG8KR_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001021554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0201667.1"
                     /translation="MNIIQQYELKYITFDQLSEEIWGYGQRLINEVGVERFSFYVEAA
                     AGYHNFRFYIFPLYI"
     gene            8549..8839
                     /locus_tag="ACG8KR_002343"
     CDS             8549..8839
                     /locus_tag="ACG8KR_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000026576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HFQ0201668.1"
                     /translation="MSINTFVYSHPINVYIIKNLGITVEQFCELYAYPQGTVASWITR
                     QRRIKSLPASFVYDLSLASSLNMSDVYEKLLSLEKEYDSFKIKHDKKIKKHI"
BASE COUNT         2815 a         1585 c         1677 g         2774 t
ORIGIN      
        1 tttttattaa gttaaagaac ggattcaatt gcagcggtca ttttcaatgg agaagtttgt
       61 ggtgcaaatc gttcgattac tttgccattt ctatcaatca aaaatttagt aaaattccat
      121 tttattgctt tagtgacagt accaggagct tcatttttaa gataagtaaa aagaggatct
      181 gtattttctc catttacgtc aatcaattga tgcataggga aggtaacgcc ataagtggtt
      241 ttacaagctt ctgctgcttc agtcgctgaa tctagctctt gtttaaattg attggaagga
      301 aagcctaaga taaccagacc ttgtgtctga taagcttggt aaagttcttc taattgttta
      361 aattgaggtg ctagaccgca ttttgttgca gtgttaacaa tgattatcgg tttgcctgca
      421 tattttttca atgaatagac agtgccgtct tctaaagtag tttcaaaatt ataaatagac
      481 ataaagtttt ctcctttcta ttttatataa taaaaagagc gctaaactga aaactagcta
      541 tttgttttcc cgtttagaac tcttttcaga caataaatat ttaataaaat acgaagataa
      601 gatagctata ttgcttctca tctttacgtt cttttagaaa ctatgcttct acttcagacc
      661 attcatcgcg ttttgctaaa aattcacgag ccaaagcttg tgcaccttct agtgtatggc
      721 tggcagccca gccacattgt gtttcattgc ttgcaggtac ttcagtagct tcaagaacat
      781 ctttcattgt tgcttcaaga acatctaaaa tgtcttcgta gctttcatga ttcaatacag
      841 tcaagtaaaa gcctgtttgg catcccattg gagaccagtc tacgatgtaa tctgcgtgat
      901 tgcggattag ttcggcagtc aagtgctcaa gtgaatgtag acttggcatg tccatgtgtt
      961 ctttatttgg ttgtttaaag cggacgtcgt atttaatgat gacatcgcca tgcgcacccg
     1021 tttttcgatc agctacacgt acataaggtg ctttgacttt tgtatggtca agattaaaac
     1081 tttcgacatt cattctcatt tgtaagactc ctatctgttt gttcattcgt tttgatgctt
     1141 ttcactaaag aattatttag tgaaatttac aaaaacgtct ttctacttga ttctaacact
     1201 tatcgtttcc aaggtgtagc aatctgaaat attttttaga tttttctgct aagtcgttta
     1261 ttgggacttc tagttcttga ttctgtcaaa tgcctgagct aaatcgttta tgagatcctg
     1321 accatactca attcctacag aaagacggat caagccgtcc tttatgccag cctcttctcg
     1381 tttttctttt ggaatagacg catgagtcat gacagaagga atttccacta aactttctac
     1441 gccacctaaa ctttctgcta aagtaaatag ttggagtgat tcaacaaatg gaatagcaga
     1501 ttcttcattt tttaaagtaa aagaaatcat tccgctaaag ccgctcatct gctgcttagc
     1561 aactccatgg agttcatgat cgggtaatcc tggatagtag accttttcta ctgctggatg
     1621 agaaaataga aaatcagcta ccacaaaagc atttttttga tgttcctcca tacgaacact
     1681 tagtgtcttg attccgcgct gaagtaacca gctatcttgg ggaccaagta cggctccgat
     1741 agcattttgg taaaatccga tctgatcggc tagatcttta tggttagaag tcactaaacc
     1801 agctactaca tcgctgtgtc cgcctagata tttggtaccg ctatggacaa cgatatcaga
     1861 accgagatct aaaggtcttt gaaaataagg agtagcaaat gtattgtcag caataactat
     1921 caatccatgt tgatgagcca gagtggcact tttttctaaa tcagtaatct ttagaagcgg
     1981 attgctaggt gtttcaagat aaagtgcttt tgtattgggt ttgatggatt gttctatttt
     2041 atcaagatta ctcgtatcta tgatcgtgta ctcgagacca tttttagtaa gaactttgtc
     2101 aaatagacgg aatgttcctc catagacatc atctcctaat aagatatgat cgccagcttg
     2161 aaaaagagaa aaaacggcat gaatcccaga aagaccagaa gaaaaagcga atcctcgaac
     2221 accgccttcc aaatcagcaa tcagttcttc caatgcaaaa cgagtaggat taccagatct
     2281 tgaatattca tattgtttag gctggccgac gccatcttgc cgataagtgg atgtttgata
     2341 aataggtaca ctgacagctc ctgttgtggg atcttcactg atgcctccgt ggatcaattt
     2401 tgtttgaatg tgcatgtttc tttcctccta taagtaaatg ttttttgata aatagcggtc
     2461 tgctgcgtct ggaaaaatcg taacgacacg atgaccaggt ggtaaacgac gaatttctct
     2521 taatgctgct gcaaaagccg caccactcga acttccaact aatagtcctt gttctcgtgc
     2581 taactgccta gtatagttaa acccttctac atcagagatt gtctcgatct gattgattga
     2641 aaggggagaa agaaacggcg gaatgaattc tacaccgata ccctcaattt cgtgaggggc
     2701 tggatcacca ccatttaaca cagagccctc tggttctaca ccgattattc gaatgtcggg
     2761 atatttttct ttcaaaaagg tagaagttcc agcaaaggtt ccaccactcc caattcctgc
     2821 tacaaaacta tggatatttt ctttgatttc atgaaagatt tctggcccta acgtatgata
     2881 gtaagcagca ggattatctt tattttcaaa ttgaagaggc agatagctgt ttgatatctc
     2941 ctctgcaagt tttttacttt tgctgatggc accgatgatt ccttgctcac taggagaatg
     3001 gatgatcttt gctcccaatg ctttcatcaa ttgttgtttt tctaagctaa acttctctgg
     3061 aacaacaaaa accgttctta atttgtatgt gagtgcggcc agagcgatcc caattcctgt
     3121 atttccggca gttggttcga tgatcgtcgt tttttcattg atttttcctt gggcaaatcc
     3181 ttctttgatc aaataactgc ccaatctatc tttgatactt cctccaggat ttaaatattc
     3241 tagttttgca taaatagcag aacctttagg catagaatag tgctgattgt caaagcgata
     3301 aagcggtgtg gaaccaatag cttctgtaat tgtcgagata atcatgtggg cctcctaaaa
     3361 aaagataata aaaaaaggag ctgactatta aatcagctcc ttgaaagaca tagagaaaac
     3421 ctgaataatt catactttta attaaaacca tgtgaaactg cctcacggca gcttacaatt
     3481 actttttcat cttcacccgt taagtgatga aaaaagaacc cctttctgct atggttaaag
     3541 tgactaaacc aaccaaaaga aaggagttct tttcatgact agcttacaca aaaaccaagt
     3601 aaaattcaat tcaaatatca ctatttctca tacaggtggt caattatcga gtgattcggg
     3661 tctcgtccta gtgaaagagc tgatgaacat ctttggtttt tctgaactgg ctaagcaaca
     3721 cattcacatt gaagacgaac gagcgtattt tactcatgac aacttatcca tacttgagca
     3781 gttcattatg caattaattg ctggttactc agctgattct gcagctaatc tcctgagaca
     3841 agatcctgtg tttaaagctg ttttagatag gaaagaactc gcttctcaat cttcactttc
     3901 ccgattttta gatcgactat ctgaggagaa tatccatgaa cttcaagctt tgaaccaaga
     3961 acttatcgat aaagcacgcc tcatccgtaa tgatacggaa ttaatcattg atttagattc
     4021 gacccattca gatacatttg gtcaccaaga acaaacgaat tataatacac actaccaaac
     4081 ctatggttac catccattgg tagcttttga cggattgact ggtgacttct taaaagctga
     4141 actacgttca ggaaatcaat atacatcaaa aggcgtaaag gagtttctca cacctttatt
     4201 agagcactat aatcactctc taccaaacac tgacatcttg gttcgtggag acagcgggtt
     4261 cgctacacct ggtgtgtatg attcgtgtga atcaaaaaag agtcattatg ttattcgact
     4321 gaagaataat cgtagactag gtcaaatagc tgagaaatca gtactttatg gcgataatca
     4381 aaagtgggaa gaacgagaag ttcagtactt ctcgaccact tatcaagcac aatcctggtc
     4441 acaaagtcgt cgcgtgtgta tacgctcaac acgtgaagcg ggcgaactac tctttcgaca
     4501 tgaatttatc gtgacgaatc tatcagaaaa tgtttctcct gataccatct tttctatcta
     4561 tgccaaacgt ggcacaatgg agaacttcat taaagaagcg aaagctggct tttactttga
     4621 caaaacagac agtcctcgct ttttggagaa tcatgtccga atgatgctca gcttgatagc
     4681 ttacaactta gtcaacttct taagaacgat tggctttgaa gaagtccaaa agggaatgac
     4741 cattcattct attcgattga agtttctgaa agttgctggg aaattagtcc aaacgggtag
     4801 acgagtctat ctcaaattat ctagttatca tgtgtatcag aatgaattct acagggtctt
     4861 tgctcgcctg aggcgagcca gtcaatggat ctaatccaac aatctaacga tttttttact
     4921 gggaagttat ccagggagaa gtatgctcaa aatgccttac acccaaaatt tattcctcaa
     4981 tatttttgaa cagtgaatgt ttgaagtaac tttttagtca aaatcagttg atagggatat
     5041 tagtgataca tatttcaata aaatgtgcca aagaattaaa gctatgaatt attcagggat
     5101 agctttatgt atagagattt ttatgaagaa tctatagaat tgactccaac ggaaccaaat
     5161 aaatctcaaa tcactttgtc aaaggagcta gagagaaaag tagagtctca agaactagct
     5221 ataggaatta gtataaccaa tgtgtcgaca tacaaagaat tatacgaagc taaatcacaa
     5281 gataataagg attttgtaga aaatatagat aaggaaccaa caaatacagt aggggattaa
     5341 cgtaaataag tgaagattat attaacagac tctttattta gttaagtaaa aaatggtttg
     5401 ttggaatttt atgctttatc tgttctgagt aagacttaat tttatagata gtaattatat
     5461 gtaggaaatt aaagatgaga agataataaa agcagcatga tcaactattt gtcattgacc
     5521 atgctgtctt tttttattct atacacggtt tgtctcgtaa cattgtagtc tttagcaatc
     5581 ttagcaatcg caacaccatt tttaagatct tcaacgatgc ttttgtaaac aaggcgacgt
     5641 tggggatctt ttgcgtctgc actgtataat ttgggccgcc ctttgtagac gccgtttgct
     5701 tttgcgatct taattccttg tgcctggcga cgtttcgatt cagtccgttc ttgttcagcg
     5761 atcatggcca aaatttggat gatcagatcc ttaatgaatt tatccaggag aggattgcca
     5821 attgcttcag ccatgatcgg taaactagta atgatcagct tcacattttt cttcttcaag
     5881 taattgactg aagcgatgat ctcgtcataa tttcttccaa gtcgatcaat agcttccaca
     5941 ataaaaatat cttgatctcg gacgtaatct aatgcttctt ggaaaacagg tcgatgagta
     6001 atcgttgcgc cagattgttt ctcgatgaaa attctctctg caccgaattt tttcatttct
     6061 tcaatctggc gttgttcatt ttgatcgatc gatgagacac gtatgtatgc aactctcaaa
     6121 acttttacct actttctgca actatttaaa atacacccta atcatacacg aggaagccat
     6181 atttttcaat gttataccag tgtctattta gggtaaacct tatatagaca aaaactaatg
     6241 atggaggaaa tagtatgaga attttttgta agtgtctact gataacttat tctcaaatgt
     6301 tgtaaggatt ttattcattt gttaatatta aagaacggaa ctccaaaaaa gtccgtactc
     6361 taatttgcag tctctcactc ttttagagtg agagttttta ttagattttc aaattaatta
     6421 gtaaagtgat agaataaatt gatcatacaa atcaaaagaa gaagcagggt gatagcatac
     6481 ctatgcttct ttttttatgt atttagctat ttcttcttta caatatcgaa catatgtttg
     6541 tataatgtga aagaatggag tgttatttat gatggattat tcaaaagagc cagtaaatga
     6601 ttacttttta atcgatatga aaagctttta cgcaagtgtc gaatgtatag aaagaaattt
     6661 agatccatta acaacagaac ttgttgttat gagtcgagct gataatactg gttcaggatt
     6721 gatattagct tcttctcctg aagcaaaaaa gcggtatggt attacaaatg tgagtagacc
     6781 acgtgactta ccacaaccat ttcctaaaac actacacgtt gttccaccac gtatgaacct
     6841 atatatcaag cgaaatatgc aggtaaataa tattttcaga agatatgtgg ctgatgaaga
     6901 tctactgatt tactcgatcg atgaatcaat cttaaaagtg acccgatcac tgaatctttt
     6961 tacgactgaa ggaacacgaa gtcaacggag aaagaagctg gctcaaatga tccaggagaa
     7021 tattaaagaa gagctaggat tgattgctac agtaggtgtc ggagataatc cccttttggc
     7081 taaactagcc ttagacaacg aagcaaagca taatgaagga tttgttgcgg aatggacgta
     7141 cgaaaacgtt cctgaaaaag tatggaatat tcctgaaatg acggactttt ggggaattgg
     7201 ctctcgaatg aaaaaaagac tcaatcagat ggggattatg agcattagag atcttgctaa
     7261 ctggaatcct tatacgatta agaatagact aggtgtgatt ggcttacagc tgtacttcca
     7321 tgctaatggg atcgatcgaa cagatatagc aatcccacca gaaccgacaa aggaaaaatc
     7381 atatggaaat tcacaagtgt taccacgaga ttatactaga agaaatgaaa tcgagctggt
     7441 ggtaaaagaa atggcggaac aagtagcgat acgcattagg cagcataact gtaaaacagg
     7501 ttgtgtccat ttacacattg gcacgtcgat cttagaaacg agaccagggt tttcacacca
     7561 gatgaagatc cctatcacag ataatacaaa ggaattgcag aattattgtc ttttcttgtt
     7621 tgataagtat tatgaaggac aagaagtaag gcatgttgga ataacctata gcaagcttgt
     7681 gtacaccgat tcgcttcagc tggatttatt tagtgatcca caaaagcaaa ttgacgaaga
     7741 aaatttagat aaaattattg acaagattcg gcaaaaatat ggttttacgt cgattgtaca
     7801 tgcttcttca atgttagaag gcgctagaag cattactaga agcacacttg ttggtggtca
     7861 tgcgggcgga aatgggggaa ttaaaaatga ttgattacaa tgatcgtgga cgtttaaagt
     7921 gggcagggtt ttatttatct gatcatactg aagaaattga tgccgacaat gaacaacgaa
     7981 ccagtcaaaa tttagcaaaa gaacaaatga cgactgaaga gattgcggaa gtattgaacg
     8041 ctgcagtcat aaaaaataga tcagtatcta ttcaaaaaga agaacgaaac gctgaaggat
     8101 actatccaga agatatcatt ggcaagatct taggtagtga tgagctgggg atctacgttg
     8161 gaaaagacaa aattcactac gacgaaatac gtcatatcga attttaccag gataataaat
     8221 ggagtcgatt agaatgaaca ttatccagca atatgagcta aagtatataa cctttgatca
     8281 gctatcagaa gagatttggg gttatggcca acgtttgata aatgaggttg gtgttgagcg
     8341 cttctctttc tatgtagaag ctgcagcagg ttatcataat tttaggtttt atatcttccc
     8401 gttatatata taaggtattt tacggattct atagagttat ctctatgaat ccttttttgt
     8461 ttttacaaat aggagtagat actagaataa tgcgtgtgtt aaactattat tgcaaacgca
     8521 ataataaaag aaagtaggct tacgcaccat gtctatcaat acatttgttt actcgcaccc
     8581 gatcaatgtt tacattatca agaatttagg aatcactgta gaacaatttt gtgagttata
     8641 tgcctaccca caaggaacgg ttgcaagctg gataacccga caacgtagaa taaaatcatt
     8701 acctgcttca ttcgtttatg atttaagcct ggcctctagt ttaaacatgt ctgatgttta
     8761 tgaaaagctc ctgtctttag aaaaagaata tgattccttc aagattaaac atgataaaaa
     8821 aataaaaaaa catatataat atcaatcaca a
//
LOCUS       DAZKLD010000122         8815 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.122, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000122 DAZKLD010000000
VERSION     DAZKLD010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8815)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8815)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8815
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.122"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            94..741
                     /locus_tag="ACG8KR_002344"
     CDS             94..741
                     /locus_tag="ACG8KR_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0201669.1"
                     /translation="MEAKITLEPFERILSGYRKVEELAVNVTDCSKLAQKYARFGVEG
                     YRLGNYVGTGYLNRYLECMVDRAPMLIYRQNYLIPLLFRRSDSAFRLFEEEYRMEAFF
                     LLLEWSLKHRPEKILIERNEKIDTKKNKVIDSAYLAFRVSEILDCGGYPISNFQSIDQ
                     FIEWNRIYRLIDNGGIGRHSKVFDPEYPENMEELKMIISLVKLKYPETDLDLYIE"
     gene            complement(834..3113)
                     /gene="helD"
                     /locus_tag="ACG8KR_002345"
     CDS             complement(834..3113)
                     /gene="helD"
                     /locus_tag="ACG8KR_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase recycling motor HelD"
                     /protein_id="HFQ0201670.1"
                     /translation="MNEKQLEQTHVDETIELIQIEQNMLDTKQQELTQEMNHSTKDTA
                     NHKIRAGSNEAFYESAVEYRQHEQELLLKYHTLEAQQKRLQTLSVMKDSPYFARIDFK
                     EDRETETLYLGIASLRDLAEETIVIDWRAPIANLYYEGEIGPAFYETETDRIDVDLQL
                     KRQFKIQEGRILSMVDTSEVINDDFLLEILDESSSNHMKNIVSTIQKAQNAIIRDTTS
                     KVMLIEGIAGSGKTSALLQRIAFLLYHNRKWLEAENVLLFSPNHLFSDYISTVLPSLG
                     ESGVPTQTFKNYLVQLLPEFSLLDEQQQESGFLLGEKDPIQVMKSGLTLVDQIDRYIQ
                     GITNYGPLFRDMKINGRTILSKESIRQWYKETNEQLPLHHRLSLLQTKLLKKLGGLQK
                     DETRQQWVKDLAEEQLQELYATDPNLEYTEQKERSLRKKLANQIVRKRFRRIHRGIMN
                     YQFVNITKQYLHFLQTVPLSLLTRHKISETRWQEHLTTVRTHLRQRELRQEDAVLFFL
                     LMRRTYPVTVTHKARYIFIDEMQDFAPSQVALLQALFPKANLTFCGDLNQNVFGNETI
                     VGSLEDLFVDREVTRFQLTTSYRSTKEITDFANHFIVKEHQVETTARKGDLPLLIQGT
                     TDDIKLNWLADTIRTTQGKARYWRTAIIAKTQEECEALYAQLPDELKQQVQLILNESD
                     FMKRSIILLPAFLAKGLEFDRVFLWDVDAEHFSSQQDKLILYTMCTRAMHELTLFTSN
                     NESPLLNNMDISSYEKKVL"
     gene            3391..5214
                     /locus_tag="ACG8KR_002346"
     CDS             3391..5214
                     /locus_tag="ACG8KR_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFQ0201671.1"
                     /translation="MTHFKKFLLTLLIGGIALLSEFVFHQPALAFAIVAFTGGILAFL
                     MFLEMIKTLRSGRYGVDVLAITAILATLAVKEYWASLMILIMLTGGDSLEDYANKRAG
                     KELQSLLDNTPRIAHRFKEGKLQDIVIDEINIDDQLLVKPGELVPADGRVTKGESSVD
                     ESSLTGESRPETKKPGDQIMSGSLNGDGSIQMIAEKRAADSQYQMIIKLVEESKEKPA
                     RFVRLADRYAVPFTLIAYLIGGISWWFSKDPLRFAEVLVVASPCPLILAAPIALVAGM
                     SRSSKNGIVVKTGTTIEKLARTKTIAFDKTGTLTKGILEVEAIQPVNGIKKEELLLYA
                     ASAEQESSHILARSLVNNVKNSQLYPVSQLKEVTGQGIQAVVNNHEVKIGRASFVSAE
                     EIETSNTAIYVTIDRSFAGTILFSDQIRPEAAKTIKELRKLDVKNLMMITGDGSAIAQ
                     TIAEKVQLDSVHARSLPQDKLTILESIPKDQRPVAMVGDGVNDAPALAAADIGIAMGA
                     HGSTAASESADAVILKDDLLRVAKAVVISRDTMRIAKQSVLIGIFICIFLMLIASTGL
                     IPALFGAVLQEVVDTVSILSALRAKKDPAIFPDSVPKTNIV"
     gene            5261..5440
                     /locus_tag="ACG8KR_002347"
     CDS             5261..5440
                     /locus_tag="ACG8KR_002347"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016862 - intramolecular oxidoreductase
                     activity, interconverting keto- and enol-groups [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxymuconate tautomerase"
                     /protein_id="HFQ0201672.1"
                     /translation="MPFVHVELVEGRSPEQLENMMKDITEAVHKNTQAPKEHIHVIIN
                     EMKKGTYGVNGEWKK"
     gene            5777..7717
                     /gene="thrS"
                     /locus_tag="ACG8KR_002348"
     CDS             5777..7717
                     /gene="thrS"
                     /locus_tag="ACG8KR_002348"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HFQ0201673.1"
                     /translation="MIKITFPDGAVKEFESGTTTLAIAESISKSLAKKALAGKVNGKL
                     VDLTRPIEEDASIEIITPDHEDALGLVRHSAAHLMAQAMRRLYPNIHFGVGPAIDSGF
                     YYDTDNGQNQVTAEDLPAIEAEMMKIVKENLPIERRVLSKQEALEIFASDPYKVELIS
                     ELPEEEVITAYQQGEFIDLCRGPHVPSTGRIQVFKLLSVAGAYWRGNSNNQMMQRVYG
                     TAFFDKKALKEYIRLREEAKERDHRKLGKELDLFMVSPEVGSGLPFWLPKGATIRRTI
                     ERYIVDKEVSLGYQHVYTPIMGDVELYKTSGHWDHYQEDMFPPMDMGDGEMLVLRPMN
                     CPHHMMVYKNTIHSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHTFVRPD
                     QIKDEFKRTLELMVAVYADFNITDYRFRLSYRDPNNTDKYFDDDAMWEKAQAMLKAAM
                     DELELDYFEAEGEAAFYGPKLDVQVKTALGTEETLSTIQLDFLLPERFDLTYVGEDGE
                     NTHRPVVIHRGIVSTMERFVAYLTEVYKGAFPTWLAPIQATIIPVSVDAHGDYAYEIK
                     ERLQMKGLRVEVDDRNEKMGYKIRASQTQKIPYQLVVGDKELEDATVNVRRYGSKETA
                     VEDLNIFIDAMEAEVKNYSREN"
     gene            7941..8789
                     /locus_tag="ACG8KR_002349"
     CDS             7941..8789
                     /locus_tag="ACG8KR_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFQ0201674.1"
                     /translation="MSVISRIESMMEDYSSAEKKLANYIIGNIEKVPTMTANELAEAS
                     GLSAPTVVRFSKKIGFQSLTDFKITISTELQTGIDEGFSDIEANESFYSIKNKLGNNA
                     QVAIKETVDILEEETIQKVVECLESAETVFLYGVGASSLVVEDILQKWSRVGKPIIFE
                     KDIHVLLPQLVSNEKKKVLWLVSNSGRSADVVALAELAKSMNIEIIALTQFGNNPLSK
                     IADVLVQTSRPKEITNRSAATNSLLAQFATIDIIFYFYMAKNEKLSEKVKKTREAIQE
                     YFMDSK"
BASE COUNT         2777 a         1571 c         1875 g         2592 t
ORIGIN      
        1 ttattttctt ttctaattca acctgtggta tgatgagagc ataagtatca tgtgaaatga
       61 tatgaagtat gtggaagatg gacggggaag aatatggaag caaaaatcac tttagaacct
      121 tttgaacgta tcttaagcgg ttaccgcaaa gtagaagagt tagctgtgaa cgtaactgat
      181 tgttcaaaat tagcgcaaaa atacgcaaga ttcggcgttg aaggctatcg attaggaaac
      241 tatgtcggta cagggtatct gaaccgttat ttagagtgta tggtggaccg cgcaccaatg
      301 ctgatttata gacaaaatta tctgattccg ctgctttttc gtcgttctga ttcagccttt
      361 cggttatttg aagaagagta tcggatggag gctttctttc tactattaga gtggagcttg
      421 aaacatcgtc ctgaaaaaat tcttatcgaa agaaatgaga agatagacac gaaaaaaaat
      481 aaagtgatcg atagtgcata cctagcattt cgtgtatcag agattttgga ttgtggcggc
      541 tatccaatca gcaatttcca atcaattgac caattcattg aatggaatcg aatttaccga
      601 ttgatcgaca atggcgggat tggccgtcat agcaaggtat ttgacccaga atatccagaa
      661 aacatggaag aactgaaaat gattatttcg cttgtcaaat taaaatatcc tgaaacagat
      721 ttggatttat atatagaata aatgacctta aatgattgaa ttactttttc tgcaattatt
      781 attgtaaata aaaagacaac tgctaacatt aatgatagca gttgtctttt tacttacagt
      841 acttttttct cataagatga aatatccata ttgtttaaca aaggactttc attattggag
      901 gtaaaaaggg tcaattcatg catcgcacgt gtacacatgg tgtaaaggat caatttgtct
      961 tgctgagaag aaaaatgttc tgcatctaca tcccacaaga aaactcggtc aaactccaga
     1021 ccttttgcta aaaaggctgg aagtaaaata atggaacgct tcataaagtc actctcattt
     1081 aaaatcagct ggacttgttg tttcaactcg tctggcagtt gagcataaag agcttcgcat
     1141 tcttcttgtg ttttagctat gattgctgta cgccaatacc gggcttttcc ctgagtcgtt
     1201 cgaatggtat ctgctaacca attcaatttg atatcatctg ttgttccttg gatgagcaaa
     1261 ggaaggtctc cttttcttgc tgttgtttct acttgatgtt ctttcacaat aaaatgatta
     1321 gcaaaatcag tgatttcttt tgtggagcga tagcttgtcg ttaattggaa acgagtgact
     1381 tcacggtcca cgaaaaggtc ttccaatgat ccaacgatcg tttcatttcc aaagacattt
     1441 tgatttagat ctccacagaa agtcaaattt gcttttggga acaaggcttg cagcaatgcc
     1501 acttgtgatg gtgcgaaatc ctgcatttca tcaataaaga tatagcgagc tttgtgtgta
     1561 accgtcactg gatatgttcg gcgcatcagt aaaaagaata gtacagcatc ctcttgacgc
     1621 aattctcttt gacgcaagtg tgtacgaaca gttgtcaaat gttcctgcca cctggtctcg
     1681 ctgattttat gtcgtgttag aagagaaagt ggcacagttt gtagaaagtg gagatattgt
     1741 ttcgttatat tgacaaattg ataattcatg atccctcgat ggattcgacg gaatctttta
     1801 cgtacgattt ggttggccag ttttttacgt aatgaacgct ccttttgttc tgtatattca
     1861 agatttggat ctgttgcgta tagttcttgg agctgttctt ccgccagatc tttgacccat
     1921 tgctgacgtg tttcgtcttt ttgcagaccg cctaattttt tcaggagctt cgtttgtaat
     1981 agagaaagtc tatgatgtaa aggtaattgt tcatttgttt ctttatacca ttggcgaatc
     2041 gattcctttg ataggatcgt acggccattt attttcatat cacggaacaa gggaccgtag
     2101 tttgtgatgc cttggatata gcgatcaatc tgatcgacta aggtcaatcc gcttttcatc
     2161 acttgtatcg gatctttttc ccctagcaaa aaaccacttt cttgttgctg ttcatctagt
     2221 aaagaaaact ctggcagcag ctgcaccaaa taatttttga atgtctgtgt aggcacgccg
     2281 ctttcaccta atgaaggcaa tacagtcgag atgtaatcag aaaacaaatg gttgggtgaa
     2341 aatagaagaa cgttttcagc ctcgagccac ttgcgattat gatacaacag aaaagcaata
     2401 cgttgaagca aagcggatgt ctttccactt ccggcgattc cttcaatcag cattacttta
     2461 cttgttgtgt cccgaatgat tgcattttgt gctttttgta tcgtagagac gatgtttttc
     2521 atatggttgc ttgatgattc atccaatatc tctagcaaaa agtcatcatt gattacttcc
     2581 gacgtatcta ccatagataa aatacgtcct tcttggattt taaattgtcg tttcaactgc
     2641 agatcgacat ctattctatc tgtttcagtt tcataaaatg cgggaccaat ctcaccttca
     2701 taataaagat tggcaattgg tgcgcgccag tcaataacga ttgtttcttc tgccagatct
     2761 cttaaagaag cgatacctag atataatgtt tctgtttcac ggtcttcttt aaagtcgata
     2821 cgggcaaaat aaggactatc tttcatgaca gacaacgttt gcaggcgttt ttgctgtgct
     2881 tcaagagtat ggtatttcaa aagcaattcc tgctcatgtt ggcgatattc aacagcagat
     2941 tcataaaaag cttcattgga accagctcga atcttgtgat tagctgtgtc ttttgtcgaa
     3001 tgattcattt cctgagtcaa ttcttgttgt tttgtatcca gcatattttg ttcgatctgg
     3061 attagttcga tcgtttcatc cacgtgcgtt tgttctagtt gtttctcatt catcgcatgt
     3121 ttgctccttc cgccgtaaat aacgaattaa aagtatagca tagtttttcg aaaaaaggta
     3181 ctttttaact gatgaatcga aaaagtaatc cttgctgaat atttttgttt gtagaactga
     3241 tgaattttgg aaacgctgat agtgatagtt gttttcaatt aaggcctatt gttttaaaaa
     3301 gtgctagtca aggactatgc ttaagatgaa agaggcagct ggaaatttat tactaacctc
     3361 tctaagtcac ataaaagagg agaaaaaaca atgacacatt tcaaaaaatt tttacttact
     3421 ttgcttattg gtgggatagc tttattaagt gaatttgttt ttcaccagcc ggcccttgca
     3481 tttgcgattg tcgcatttac tggagggatt ctcgccttct tgatgttttt ggaaatgatc
     3541 aagacgttac gttcaggaag atatggcgta gatgtattag cgattaccgc aatccttgca
     3601 acattagccg tcaaagaata ctgggctagt ttgatgatat tgatcatgct tacgggcgga
     3661 gatagtctag aggattacgc gaataaacga gcgggtaaag aactgcagtc tttgttagat
     3721 aacaccccaa gaattgccca tcgattcaaa gaaggaaaat tgcaggatat agtaatcgat
     3781 gaaataaata ttgatgacca gttactagtc aagcctggcg aacttgtacc cgcagatggt
     3841 cgagtgacca aaggagaatc ttcggtcgat gaatcttctt tgacaggtga gtcccgcccg
     3901 gaaaccaaga aacctggaga tcagattatg tcagggtctt taaatggaga cggatcgatc
     3961 caaatgatcg ctgaaaaacg tgccgcagac agccagtatc aaatgattat taaactagtc
     4021 gaagaatcaa aagaaaagcc tgcccgattt gtccgtttag ctgatcgtta tgcagtccct
     4081 ttcacgttga ttgcttattt gatcggtggt atttcttggt ggttctcaaa agacccttta
     4141 cgatttgctg aggtactggt agtggcatca ccttgtccac tgattctagc tgcgcccatt
     4201 gctttagttg caggaatgag ccgttcaagt aaaaacggga tcgttgtaaa aactggaaca
     4261 acgattgaaa agctcgcacg tacaaaaacg attgcttttg ataaaacagg aacgcttacc
     4321 aaagggatat tggaagtaga ggcgattcag ccagtcaatg gaataaaaaa agaagagcta
     4381 cttctgtatg cagctagtgc agaacaggaa tctagccata tcttagctcg ttcgttagtg
     4441 aacaacgtga aaaacagcca actgtatcct gttagtcaac tgaaagaagt gaccggtcaa
     4501 ggaatccaag cagtagtcaa taatcatgaa gtaaagattg gacgtgcctc ttttgtttca
     4561 gctgaagaaa tagaaacgtc caatacagca atctatgtaa caattgatcg atcttttgca
     4621 ggaacgatcc tattttcgga tcagattcgc ccagaagcag caaagacaat caaggagcta
     4681 agaaaacttg atgtgaaaaa tttgatgatg atcaccgggg atggatctgc cattgctcaa
     4741 acgattgcag aaaaagtcca attagattct gtacatgccc gtagcttgcc tcaagataaa
     4801 ctgaccattt tagaaagtat ccctaaagat caaagacctg ttgcgatggt aggagacggc
     4861 gtgaatgacg caccagcact tgctgctgca gatatcggta ttgcaatggg ggctcatggt
     4921 tctacagctg ctagtgaaag tgcagatgcg gtcatcttga aagatgatct cttgcgtgta
     4981 gcaaaagcag tagtgatttc aagagatacg atgagaatag cgaaacagtc tgtactgatt
     5041 ggcatcttta tttgtatttt tttgatgtta attgcaagta cgggactaat ccccgcatta
     5101 ttcggagctg ttttgcaaga agtcgtggat acggtctcta ttttgagtgc cttgagagca
     5161 aaaaaagatc cagccatttt tcctgattca gtacccaaga caaacatagt atgatagaat
     5221 cgagttgaaa aaagaacgat cagaaagagg agagagcatt atgccattcg tacatgtcga
     5281 attagtagaa ggtcgcagtc cggaacaatt agaaaatatg atgaaagata tcacggaagc
     5341 tgttcataaa aacacgcagg caccaaaaga acacatccat gtcatcatca atgagatgaa
     5401 aaaaggaaca tacggtgtaa atggggaatg gaaaaaataa gtttagtttc ccacttgatt
     5461 tttctattta ttagtggtta aataaacaca taattgattg atgagaaagc caactgaatt
     5521 tgagagtctt cgataagaga ggaaaagcta ggctgaaact tttccaggag aaaaccaaag
     5581 gatgaccact ttcgaaaccc ttgttgaagc aagggcggtc ggaccgttac aacgcttgag
     5641 gaacgcattt gcgtttgaac attaggtgga accacgaact tttcgtccta gtatctcttt
     5701 tcaagagatg ctaggatttt tttgtttttc agcttgatga tctgatcaat caaaacattt
     5761 caaggaggaa actattatga taaaaattac ttttccagat ggtgcagtca aagaatttga
     5821 gtctggaaca acaacattgg caattgctga aagcatttca aaaagcttag caaaaaaagc
     5881 tttagctggt aaagtgaacg gaaaactcgt tgatttgact cgtccaatcg aggaagatgc
     5941 aagtattgag atcatcacac cagaccatga agatgcactt ggcttagtcc gtcattcagc
     6001 agctcatttg atggcccaag ccatgcgtcg cctctatcca aatatccact ttggcgttgg
     6061 accagctatc gattcagggt tttactacga tacagataat ggacaaaacc aagtaactgc
     6121 agaagatttg ccggcaatcg aagcagaaat gatgaaaatc gtcaaagaaa atctgccgat
     6181 cgaacgccgt gttctttcaa aacaggaagc attagaaatt tttgcaagtg atccgtacaa
     6241 agtagaactg attagtgaat tgccagaaga agaagtgatt actgcttatc aacaaggcga
     6301 attcatcgat ttgtgtcgtg gaccacatgt tccatccact ggccgtatac aagtatttaa
     6361 attattatca gtggcaggtg cttattggcg tggaaactca aacaatcaaa tgatgcaacg
     6421 tgtatacggg acagcctttt ttgacaagaa agctttgaaa gaatatatcc gtctccgtga
     6481 agaagcaaaa gaaagagacc atcgtaaact aggaaaagaa cttgacttgt tcatggtttc
     6541 cccagaagta ggttctggtt tgccattctg gcttccaaaa ggggcaacaa tccgacgcac
     6601 gatcgaacgt tatatcgtag acaaagaagt tagtctaggt taccaacatg tatatacacc
     6661 aataatggga gatgttgagt tgtacaaaac atctggacac tgggatcatt atcaagaaga
     6721 catgttcccg ccaatggata tgggagatgg cgaaatgctt gttcttcgcc caatgaactg
     6781 ccctcatcac atgatggtct acaaaaacac gatccattct tatcgtgaat taccaattcg
     6841 tatcgctgaa ttaggaatga tgcatcgcta tgaaaaatca ggtgctttgt caggtttaca
     6901 acgtgtacga gaaatgacgt tgaatgatgg tcatacattt gttcgtcctg atcaaatcaa
     6961 agatgaattt aaacgtacgc ttgaattgat ggtggcagta tatgctgact tcaatattac
     7021 agattatcgt ttccgcttga gctatcgcga tccaaacaat acagataaat attttgatga
     7081 tgacgcaatg tgggaaaaag cccaagcaat gctgaaagcc gctatggatg aattagaact
     7141 agattacttt gaagcagaag gcgaggcagc cttttacgga ccaaaactag atgttcaagt
     7201 caaaacagct cttggtacag aagaaacatt atcaactata caattagact tcttattgcc
     7261 agagcgcttt gatttgacat atgttggaga agacggggaa aatacgcatc gtccagtagt
     7321 tatccaccgc ggaattgttt ctacaatgga acgttttgtc gcttatttaa cagaagtata
     7381 caaaggcgct tttccaacat ggttggcacc tatacaagca acaatcatcc ctgtttctgt
     7441 tgatgcacat ggcgattatg cttatgaaat caaagaacgt ttgcaaatga aggggttgcg
     7501 tgtagaagtt gatgatcgta atgaaaaaat gggctataag atacgtgcct ctcaaacaca
     7561 aaagatccct taccaattag ttgttggaga caaggaattg gaagatgcaa cagtgaacgt
     7621 acgtcgttat ggaagtaaag aaacagctgt agaagatttg aatatcttta ttgatgctat
     7681 ggaagcagaa gtcaaaaatt acagcagaga aaactaatag gtaaatttcc tgtaaaaaag
     7741 tagtagatag aaagcaatcc gaacaataga taacaggtat tcctcaaaga cacagatgct
     7801 caatagaatc tgtgggattg atgggataag atattctgtt gggcggattg tttttttgtt
     7861 ttttatccag tatacgaccg tgaataaggg tctgatatgg tatacttaca tagactatca
     7921 ttaaaagggt ggaggaactt atgagcgtaa ttagtcgtat tgaaagtatg atggaagatt
     7981 attcttcagc agaaaaaaag ttggccaact acattatcgg taatattgag aaggtcccta
     8041 caatgacagc taatgaatta gcagaagctt ccgggttgag tgcgccgaca gttgtacgtt
     8101 tttccaaaaa aatcggtttt caaagtctga cggacttcaa aatcacgatt tcaacagaac
     8161 tacagactgg aatcgatgaa ggttttagtg atatagaagc aaatgagtcc ttttactcta
     8221 tcaaaaacaa acttggtaac aatgcacaag tagcgataaa agaaacagta gatattctag
     8281 aagaagaaac gatccaaaaa gtcgtagagt gtttggaatc ggcagagact gtttttttat
     8341 atggggttgg cgcctcttct ttagtagtag aagatattct gcaaaaatgg tctcgtgtcg
     8401 gtaagccaat catttttgaa aaagatatcc acgtcttgct cccgcaactt gtcagcaatg
     8461 agaaaaagaa agtgctttgg cttgtttcaa actcaggtag aagtgcagat gtagtcgcat
     8521 tagccgagct ggcgaaaagc atgaatatcg aaatcattgc gttgacgcaa tttggaaata
     8581 acccgcttag caagatagcc gatgtactcg tacaaacatc tagaccaaaa gaaatcacga
     8641 atcgaagtgc ggcaactaat tcacttttgg cccaatttgc tacgatcgat atcatttttt
     8701 atttctatat ggctaaaaac gaaaagctta gcgaaaaagt gaaaaaaaca cgagaagcaa
     8761 tccaagaata ttttatggat tcgaaataaa aaccttacta ttttttatat aagtt
//
LOCUS       DAZKLD010000123         8704 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.123, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000123 DAZKLD010000000
VERSION     DAZKLD010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8704)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8704)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8704
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.123"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            123..1268
                     /locus_tag="ACG8KR_002350"
     CDS             123..1268
                     /locus_tag="ACG8KR_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFQ0201675.1"
                     /translation="MATIKQYEKKDGTKAWMFQTYLGISPLTGKEVRTTRRNFKTKKE
                     AQIELNRLLVDFEENGLKRETNETFQEVYDLWYESYKTTVKEVTQLKIEIKFNKWILP
                     KYGQFRIKEITTKHVQKILNQWAKTTDQYKVLHSTVSRIFKYAMTLGLITKNPCELVI
                     MPNRNVEKKKDTVKIYSRDQLETLFQYLESKRSTYRAEYDKTLLRFLFFSGCRISEAL
                     ALDWSDIDFDSNTVTINKTLSQTKYGYKISSPKTRNSYGSLSLDNKTINWLKKWQINQ
                     KRYMLTLGITDPTMIFCGIYKEIITKHAIYSRMITICKNSGIPFLGNHVTRHTHASML
                     LESGVSMKEVQERLRHSSIKLTMDTYAHLTKETKEKTVEKLAEHLNF"
     gene            complement(1353..1658)
                     /locus_tag="ACG8KR_002351"
     CDS             complement(1353..1658)
                     /locus_tag="ACG8KR_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HFQ0201676.1"
                     /translation="MTEHNHSHDHEGHEHITLVDDQGNETLYEILLTVDGQEEFGRNY
                     VLLYPAGVSEDEDVELQAYAYIENEDGTEGELEQIETDAEWDMIEEVFNTFMAEEEE"
     gene            complement(1683..2114)
                     /gene="ruvX"
                     /locus_tag="ACG8KR_002352"
     CDS             complement(1683..2114)
                     /gene="ruvX"
                     /locus_tag="ACG8KR_002352"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HFQ0201677.1"
                     /translation="MRVMGLDVGSKTVGVAISDPFGWTAQGVEIIRINEAEEEFGFDR
                     LKELVAQYEVDRFVVGLPKNMNNSIGPRAEASMSYGEKIKELFSLPVDYQDERLTTVQ
                     AERMLVEQANTSRAKRKKVIDKLAAVMILQNYLDGSGKQLF"
     gene            complement(2111..2377)
                     /locus_tag="ACG8KR_002353"
     CDS             complement(2111..2377)
                     /locus_tag="ACG8KR_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HFQ0201678.1"
                     /translation="MGFTDETVRFGFDDSRKKEVSETLAIVYRALEEKGYNPINQIVG
                     YLLSGDPAYIPRYRDARNLIRRHERDEIMEVIVKDYLSNHGVNL"
     gene            complement(2632..3357)
                     /locus_tag="ACG8KR_002354"
     CDS             complement(2632..3357)
                     /locus_tag="ACG8KR_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HFQ0201679.1"
                     /translation="MSKKTTKAIVGIVAAVIAGAFGVSTTQKDSDIFQQITTIFDSTP
                     QASQVADVPEYDGKHQEIEINGNQPEFTKEDLSLSKGTWESYSNIDKLNRVGQANAML
                     GKDLFPTEKREPLYIDPTGWKQKKLSDGQWLYNRSHLIGFQLTGQNNNIKNLMTGTRS
                     LNAPYMLAHENDIAAYIKETNHHVRYRVTPHFEGNELVARGVQLEAESIEDKKIEFNV
                     FIYNVQDGYTINYETGQATKTKN"
     gene            complement(3718..3788)
                     /locus_tag="ACG8KR_002355"
     tRNA            complement(3718..3788)
                     /locus_tag="ACG8KR_002355"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3754..3756),aa:Gly,seq:ccc)
     gene            3905..4657
                     /locus_tag="ACG8KR_002356"
     CDS             3905..4657
                     /locus_tag="ACG8KR_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HFQ0201680.1"
                     /translation="MTGKELDLLLDTCLLAGKIMMESNAEMYRVEDTMSRIALASGNF
                     RLVSYVTQTGLFIGLDRTSTIRMEQITNRSINLEKVVKVNDLSRKYVASELTLEELYC
                     ELQAIDRDRSFFPVWLQIVSAAVISGTIMVLFGGKMTDLPVTLVIGGLGYAAYLYSLK
                     FFRIKFLSEFLSSLLIGIAAIYSVRFGLGSNQDLIIIGCVMPLVPGVQITNAIRDLLA
                     GHYVSGVSRGTEAMMTATMIGFAIAFIFQLFY"
     gene            4673..5152
                     /locus_tag="ACG8KR_002357"
     CDS             4673..5152
                     /locus_tag="ACG8KR_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HFQ0201681.1"
                     /translation="MGTIFIQFSFSFLATAAFAVITNVPRRSLICCGLSGAFGWMIYW
                     ICVQIGGSAAFGSLLGSLGVAFISDLFSKRLKMPVTIFNIPGMVPLVPGGLAYQAVRN
                     LVTESYREAAHYAVQAIMIAGAIALGLVLSEVFNHNIRNFKVKRDAIMMKRKKRLFK"
     gene            complement(5403..6983)
                     /locus_tag="ACG8KR_002358"
     CDS             complement(5403..6983)
                     /locus_tag="ACG8KR_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HFQ0201682.1"
                     /translation="MYKRQLLELIRVNLLYVNPQLTNKAREKGKKGKRLTIYLLSQNL
                     FSGLIFLLIYGFAMFAMNFVKLPGFFTYYVALFGMLGLTQSISVIYNIFFEGNDLQVF
                     LPLPFGQEQVFSAKILVVALTIIPFVFPLLVMFILTGWRADLGILLTIIISILLFSLF
                     LIILFCLASLIVFGLARTAVFKKHRKTVSSILLGSSMLIAFAGIMWMNHQTGTIENEL
                     TDRHPISILLPFYDAASQPLSQQGLLGWGILAITALGFFLAVKQLIVPKLTEQLTDSN
                     SEFKIKRTHKKNQNVHQLLFSYNAQLVKEPNLIMQVLSSSLLTPIIFILAFALGGEIK
                     LTDLDNKLIGVVFLVGIALAFLTVNQTSFISNLISLDQENFLFIRSLPISMNQYLKEK
                     FRFGWILQSFLTGGIALLSGLSFQLPLFFIPSLLFGSLFGCYLLSLRYFARDYRLLLL
                     NWTNINQLFNRGSGSLGLVATMMGSLIISIILIVLYGMLALFYPFWLVNIPVIGLVLI
                     LSALWIVYYQRTFWKKFE"
     gene            complement(6976..7719)
                     /locus_tag="ACG8KR_002359"
     CDS             complement(6976..7719)
                     /locus_tag="ACG8KR_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0201683.1"
                     /translation="MIEFINVSKSYGKKQALKDLSLSIRQGEIFGFLGHNGAGKSTTI
                     KSLVSIIEPSSGTILVDGMKLTENRLSIKQKIGYVPDSPDIFLQLTAGEYWDLISAAY
                     ELDTQKKEKRLAELTALFDMYSHQNETIASFSHGMRQKTILIGTLLPDPDIWVLDEPL
                     QGLDPQAAFDLKEMMKAHAAKGKTVIFSTHVLDTAQQLCDKLAILKKGELIYQGAVSE
                     LLNGSPDETLEKIYLKMAGRQATGDGPYV"
     gene            complement(7895..8671)
                     /locus_tag="ACG8KR_002360"
     CDS             complement(7895..8671)
                     /locus_tag="ACG8KR_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HFQ0201684.1"
                     /translation="MVTRIFILDDSLVFEKMLVEILEESRILLSPWKFEIVQGLNVIQ
                     FVEFIKNNEIYSSDIFFLDIDLNAYYSGIELGEMLREKNEKCKIVYLTSHPNKAITVI
                     NRKITPSGYLIKEFTYEKNKKSIQSYLLKEKENYPVQHATKKRWTTVRYGNSDHYIHY
                     DEILYLMSMPGYKNKIGLVLKDEEILVNGTIKKYKKSLEYEYLCKDLKAYIINTENIT
                     SISRVNEIVTFANGEQLEMGKRSIDKLKNFMQEKIENESF"
BASE COUNT         2768 a         1681 c         1447 g         2808 t
ORIGIN      
        1 gatgaaataa aaacaactac attcaacaaa aaaaaatatt ttcaaaatca atatgatata
       61 gaagaataaa gattacattt tcctatcaaa agcacgccac gcaactgata ggaggaaaaa
      121 caatggcaac aattaaacaa tatgaaaaga aagacggaac caaagcttgg atgtttcaaa
      181 cataccttgg aatcagcccg ctaactggta aagaagtccg tactacacgt agaaatttta
      241 aaacaaaaaa agaagctcaa atagagctta atagactact tgtagacttt gaagagaatg
      301 gacttaaaag ggaaacgaac gaaactttcc aagaagtgta tgatctctgg tatgaaagct
      361 ataaaactac tgtaaaagaa gtcactcaat taaaaattga gatcaagttt aataaatgga
      421 ttcttccaaa atatgggcag tttagaatta aagaaattac tacaaaacat gttcagaaga
      481 tattgaatca gtgggctaaa acaacagatc aatataaagt cctacattct accgtaagcc
      541 gtattttcaa atacgcaatg actctagggc tgatcactaa aaatccctgt gagctcgtaa
      601 tcatgcccaa cagaaatgtt gaaaaaaaga aggatactgt caaaatttat tcacgagatc
      661 aattagaaac cttatttcaa tatttggaat ccaaaagaag tacgtatagg gcagagtacg
      721 ataaaacttt actgcgcttt ctatttttta gtggctgtcg aatcagtgag gctttagcct
      781 tagactggtc agatattgac tttgattcaa acaccgtcac tataaacaaa accttaagcc
      841 aaacaaaata tgggtacaaa atttcaagtc caaaaacgag aaatagctat ggttccctat
      901 ctttagacaa taaaaccatt aattggctaa agaaatggca aatcaatcaa aaaagatata
      961 tgttaaccct cgggattact gatccaacaa tgattttttg tggaatctac aaagaaatca
     1021 ttactaaaca cgctatctat tctcgtatga tcacaatctg caagaattct ggtattccat
     1081 ttctaggtaa ccatgtaacc agacacactc atgctagtat gctgttagag tcgggagtct
     1141 ccatgaagga agttcaagaa cgccttagac actcatcaat caaattaaca atggatacct
     1201 atgctcatct aactaaagaa accaaagaaa aaaccgttga aaagcttgca gagcacctaa
     1261 atttctagcg gaacgtattc aatcccgtat tcaattcaaa acaaaaaaaa gctgaaccct
     1321 tatctaccaa gggttcagct atcatttaca gcttattctt cttcttccgc catgaatgta
     1381 ttgaatactt cttcaatcat gtcccattct gcgtctgttt cgatttgttc taactcgcct
     1441 tctgttccat cttcgttttc gatgtatgca taagcttgta attctacatc ttcatcttct
     1501 gaaacaccag caggatataa aagaacatag ttacgtccaa attcttcttg tccgtcaact
     1561 gttagtagga tttcatataa tgtttcattt ccttggtcat ccaccaatgt gatgtgttcg
     1621 tgtccttcat ggtcgtggga atggttatgt tctgtcataa gattacctct tttcttttat
     1681 tattaaaata attgcttacc tgagccgtct aaataatttt gtaagatcat cacagctgct
     1741 aatttatcga tgactttttt tcgtttggct cttgaagtat ttgcctgctc tactagcata
     1801 cgttctgcct gaacagtggt taatcgttca tcttgataat caactggcaa ggagaatagt
     1861 tctttgatct tttctccgta tgacatggaa gcttctgccc ttggtccgat ggaattgttc
     1921 atatttttag gtaatccaac tacaaaccgg tctacttcat attgtgctac taattccttc
     1981 aaccgatcaa agccaaactc ttcttctgct tcattgatcc gaatgatctc tactccttga
     2041 gctgtccagc caaaggggtc actgatagca acacctactg tcttagaacc gacatccaag
     2101 cccattactc tcataaattc actccatgat ttgagaggta gtccttgaca attacttcca
     2161 tgatctcatc gcgttcgtga cggcgaatca aatttcgagc atcacgataa cgaggaatgt
     2221 aggccgggtc tccagaaagc aagtagccga caatctgatt gattggattg tagccttttt
     2281 cttccaaagc ccgataaaca atcgctaatg tttcgctcac ttcttttttg cgactatcgt
     2341 caaaaccaaa acgtacggtt tcatctgtaa aacccatgat tctacacctc tttcccgcaa
     2401 ggtaattcca gcttaccttc tacaacctta ttttactcga agaaagggaa aactacaaac
     2461 aaatattcca aacttccgaa taaattaaga aacactattt gtatgttttt ttcctttttt
     2521 ctgtagcttt atcaaaagta ctcttttcat aaagtatacg ctatatttct ttgaatgtat
     2581 gtttttacac aattttttca taatattcat aaaaaattcg aatagctgct actaattttt
     2641 tgtttttgtt gcttgtcctg tctcataatt gatcgtgtag ccatcttgaa cattgtagat
     2701 aaagacattg aattcaattt ttttgtcttc aatgctttct gcttccaatt gcactcccct
     2761 ggctaccagt tcattcccct caaaatgagg agtgacacgg tagcgtacat gatgatttgt
     2821 ttctttgata tatgccgcaa tatcattttc gtgagcaagc atgtatggag cattcaacga
     2881 acgtgtccct gtcatcaagt ttttaatgtt gttattttga ccagtcagct ggaaaccaat
     2941 taaatggctg cgattatata accattgccc gtcacttaat ttcttttgtt tccaacctgt
     3001 cgggtcaata tacaaaggct ctctcttttc agtagggaac aaatcttttc caagcatagc
     3061 attggcttgt cctactctgt tcaatttatc aatatttgaa tagctttccc atgttccttt
     3121 ggaaaggcta agatcctctt ttgtaaattc cggctgattc ccattgattt ctatttcttg
     3181 atgttttcca tcatattctg gcacatccgc tacttgagaa gcttgtggtg tggaatcaaa
     3241 aatagttgta atctgctgaa agatatctga atctttttgt gtggtggaaa caccgaatgc
     3301 ccctgcaatc acagcagcga cgatccccac gatcgccttg gttgtttttt tgctcatttc
     3361 tttactcctt tactaacagc tccctaaatc ttaacatatg ttcccctata aataaatact
     3421 agaaagaaat agaaacagca aaaaataaaa cctgttttaa gatggcttaa aaatacaaaa
     3481 aagagacgga ttcctcgtct ctaatcagtc accactctat ctgtaaggta tttattatta
     3541 ttcaaaaatt ttcttcatac tcaagacaat acactgattt cacaaccgct tggtaaacgg
     3601 tgcgcaaaag cttgcatctg cggtaataag tccatccaag ttaaaaacat aagaagttgt
     3661 ttttgcttgg ctgttcttat tacttgttgc agagcagctt tttcacaacc tattttaagc
     3721 gggtgacggg aatcgaaccc gcgacatcag cttgggaagc tgggatttta ccactaaact
     3781 acacccgcaa tacaaaaaca gtatacgcct cttttattcc ttcctcaagt ttttttattc
     3841 tttacgcttc tggtatgttt tgtagtaaaa taaaactaat caatcaatta ttggagaaga
     3901 tgctatgacg ggaaaagaat tagatctgtt gctggacact tgtttactcg ctgggaaaat
     3961 catgatggaa agcaatgcgg agatgtatcg ggtagaagat acgatgagtc gaattgcttt
     4021 agcttctgga aatttccgtt tggtcagcta tgtcactcaa accggtctct ttattggtct
     4081 tgatcgtacc tcgaccatcc gaatggaaca aattaccaac cgttcgatta atttagaaaa
     4141 agtagtaaaa gtaaatgatc tttcacgtaa atatgttgct tcagagctaa cattagaaga
     4201 actttactgc gaactgcaag caatcgaccg agaccgttct ttctttcctg tctggttgca
     4261 aatcgttagc gcggcagtga tcagtggaac aattatggtg ttatttggcg gaaagatgac
     4321 cgatttacct gtgactttag tcattggagg attgggatac gcagcttatc tatatagttt
     4381 gaaatttttt agaatcaaat ttttatcgga atttttatct tctttattga ttggcatagc
     4441 cgctatctac agcgtacgtt ttggcttagg aagcaatcag gatctaatca ttatcggatg
     4501 tgtgatgcct ttagttcctg gagttcaaat tacgaacgct atccgtgatt tgctagctgg
     4561 acattatgtc tcaggagttt ctcgcggaac agaagcgatg atgactgcta cgatgatcgg
     4621 ttttgcgatt gcttttattt tccagctttt ttattaggaa aggaacacaa gtatggggac
     4681 gatatttatt cagttttctt ttagtttttt agcgactgcg gcatttgccg ttattacaaa
     4741 tgtacctagg cgttcattga tttgttgtgg tctttctggt gcttttggct ggatgatcta
     4801 ctggatctgt gttcaaatag gcggatctgc tgcttttggt tcattgcttg gttctttagg
     4861 agtagcattc atcagcgatc ttttctctaa acgtttgaaa atgcccgtaa cgattttcaa
     4921 tattcccggg atggttcctc tagtccctgg tggtttagct tatcaagcgg ttcgaaatct
     4981 cgttactgaa tcctatcgag aagcggctca ttatgcggtc caagcaatta tgattgctgg
     5041 agcaatcgct ttaggtcttg tattatcaga agtctttaac cataacatcc gtaatttcaa
     5101 agtaaagcgt gacgcaatca tgatgaaacg aaaaaaacgg ctttttaaat aaacgaagtg
     5161 taacaaagaa aaggttgtga aagagacgtt cagccttgag cgatgacaaa atatctcaca
     5221 aacattgcga caagtaaccg caggagcaat aagaagtaat tatgagtatt aaaaaaagtt
     5281 ttgtaaaaat agcttctcac atttttacat aaaattgact taatactgag aaactgactt
     5341 ttgaacaccc tctatctgta aaaaaggggc tgcgacatga tttttgtcac ggcccctctt
     5401 ctttattcaa attttttcca aaacgttcgt tgataataaa cgatccatag agcggaaagt
     5461 attagaacta aaccgatcac aggaatattc actaaccaaa atggataaaa caaagccaac
     5521 attccgtata agacgataag aataatactg atgatcaatg aacccatcat agttgcgact
     5581 aaacccaaac ttcctgaacc tctgttaaat aattggttaa tattggtcca gttcagcagc
     5641 agcaaccgat aatctctagc gaaataacgt agtgacaata agtagcagcc gaataaagaa
     5701 ccaaatagca agctagggat aaaaaacaaa ggcagctgaa aagacagtcc gcttaataat
     5761 gcgatcccac cagttaaaaa actctgcaat atccatccga atctgaactt ttcttttaaa
     5821 tattggttca tagagattgg caaagaacgg ataaacagaa aattttcttg atccaaagaa
     5881 ataagattgc tgataaaaga agtctgatta actgttaaga aagccaatgc gatccctacc
     5941 aggaaaacaa caccgattaa cttattatct aaatcagtta attttatttc gcctcctagg
     6001 gcaaaagcaa gaataaaaat gattggagtt aacaaggaag aagaaagaac ttgcataatc
     6061 aaattaggtt cttttactaa ttgagcattg taactaaata aaagctgatg cacattttgg
     6121 ttctttttat gggttctttt tattttgaac tcactgtttg aatcagtcag ttgctctgta
     6181 agctttggta ctatcagctg ttttacagca aggaaaaatc ccagagccgt gatagctagt
     6241 attccccaac ccaataaacc ttgttgagaa agtggctgac tggcagcatc atagaatggc
     6301 aataatatcg aaatcggatg ccgatctgtt aactcatttt caatagtccc cgtttgatga
     6361 ttcatccaca tgattccagc aaatgcgatc agcatcgagc tgccaagcaa gatactagaa
     6421 actgtttttc tatgtttttt aaacactgct gttcgagcca aaccaaaaac aatcaaacta
     6481 gccagacaaa aaagaataat caaaaataga gaaaacaaaa ggattgagat aatgatagta
     6541 agcaaaatcc ctagatcagc tcgccatccg gttaaaataa acatgactag aagcggaaaa
     6601 acaaaaggaa tgatggtcaa agcaactacc aaaatctttg ctgaaaaaac ctgttcttgt
     6661 ccaaaaggca gaggcaaaaa tacttgcagg tcatttcctt caaaaaagat attataaatg
     6721 actgatatac tttgagttaa acctaacata ccaaaaagtg ccacgtaata agtaaagaaa
     6781 cctggcaatt taacaaagtt catagcaaac atagcaaatc cgtaaatcag taaaaaaatc
     6841 agccctgaaa aaagattttg agacaaaaga tagatagtta atcgctttcc cttttttcct
     6901 ttctctcttg ctttattcgt gagctgtgga ttaacatata aaagattaac tcgaattaat
     6961 tctaataatt gtcgcttata catacggtcc atctcctgtc gcttgccttc cagccatttt
     7021 caaataaatc ttttctagcg tttcatcagg tgagccattc aataattcac taactgcccc
     7081 ctgataaatc aattcacctt tcttcaaaat agccagttta tcgcataact gttgtgcggt
     7141 atccaataca tgtgtagaaa aaatgactgt ttttcctttt gcggcatgag ccttcatcat
     7201 ttctttcaaa tcaaatgctg cttgaggatc aagtccttgc aaaggttcat ccaataccca
     7261 aatatctgga tctggcagca acgtgccgat caaaatcgtt ttttgacgca ttccatgtga
     7321 aaaacttgcg attgtttcat tttgatgact atacatatca aaaagcgctg tcaattctgc
     7381 taaacgcttt tctttttttt gtgtgtctaa ttcataggca gctgaaatca agtcccaata
     7441 ttctccagcc gtcaattgta aaaaaatatc tggggaatca ggcacataac cgattttttg
     7501 tttgatactc aatcgatttt ccgtcaattt cattccatct actaaaatcg ttcccgatga
     7561 tggttcaata atactaacta aacttttaat cgtagttgat tttccggcac cattatgtcc
     7621 taaaaatcca aaaatttccc cttgtcttat cgagagagac aagtctttta atgcttgttt
     7681 tttcccgtaa cttttactta cattgatgaa ttcaatcatt tctgtactcc ttttgtttgt
     7741 tattactttt tattgtaccc ttcgttattg gtggagtaaa tgtcaaaaag agtgcataac
     7801 aaaaaaggat agaaacgact aggctgctac ttgggttgtt ctcttctaca ggaaaacact
     7861 cttctaacag gtaagatatg agtctttgtt ttctttaaaa tgattcattt tctatctttt
     7921 cttgcataaa attctttagt ttatcaatac ttctctttcc catctctagt tgttctccat
     7981 ttgcaaatgt tactatttcg tttactctgc taatagatgt aatgttttca gtattaatga
     8041 tataagcttt taaatcttta cataaatact catattcgag tgatttttta tattttttaa
     8101 ttgtgccgtt tacgagaatc tcttcatctt tcaatactag gcctatttta tttttataac
     8161 ctggcatgct cattagataa aggatttcat cgtaatggat atagtgatcg ctattaccat
     8221 aacggacagt tgtccacctt tttttcgtag catgttgaac aggatagttt tctttttctt
     8281 tcaataagta actttggatg ctttttttat ttttttcata agtgaattct tttatcaaat
     8341 aaccactagg cgtaatcttt cgattaataa cagttattgc tttattcgga tgagacgtta
     8401 gataaacaat tttacacttt tcattctttt ctctaagcat ttcgcctagt tcgataccgc
     8461 tataataggc gttcaaatcg atgtctagga aaaaaatatc tgaggaatag atttcattat
     8521 ttttaatgaa ttcaacaaat tgtataacgt tcaatccctg aacgatctca aatttccaag
     8581 gacttaataa aattctggat tcttccaaaa tttctactag cattttttca aaaaccaaac
     8641 tatcatccaa aataaatata cgcgttacca taaaaagtac ctccactcga tattttacta
     8701 tcaa
//
LOCUS       DAZKLD010000124         8542 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.124, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000124 DAZKLD010000000
VERSION     DAZKLD010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8542)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8542)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8542
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.124"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            105..356
                     /locus_tag="ACG8KR_002361"
     CDS             105..356
                     /locus_tag="ACG8KR_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0201685.1"
                     /translation="MRKVLVFNTRGQSFLGIEGTLVTVDGWCDCFEVGKHVELMAFTE
                     LKDKNNVEFFESDIGWDDHEEVYGKFILRKGNSYTNGEA"
     gene            469..711
                     /locus_tag="ACG8KR_002362"
                     /pseudo
     CDS             469..711
                     /locus_tag="ACG8KR_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010708076.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            801..1040
                     /locus_tag="ACG8KR_002363"
     CDS             801..1040
                     /locus_tag="ACG8KR_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0201686.1"
                     /translation="MIDISLPEDLKPDPVTIGNSVMIFDSLDEAMSYGWKEIKDEGSN
                     WYGMRFGDGEVAKKAGIPIGKWTVDFHEAGSFFVL"
     gene            1187..5470
                     /locus_tag="ACG8KR_002364"
     CDS             1187..5470
                     /locus_tag="ACG8KR_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010746400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VaFE repeat-containing surface-anchored protein"
                     /protein_id="HFQ0201687.1"
                     /translation="MYAKTKMNWLKALALVAFSQFCFLVGTAQAHAEETTAIQPTMEA
                     IAQVASEVTEVQPAEAGVPAGEATVEQAPVTEAPTVNSKTTLDQTIGETQHPGNSPGT
                     SQPVEETASITTPASETSTLEGEKAAEATPSVQPESSEKQAEEKKRIEETVEQPAATA
                     DKEGKNEENVEKTISSTAASQNQPTKPNKESTASIENQKKKEQNIPKTGTPTLLVTVP
                     TNRSVRVRRATNAAVLGGRKIDWGENIGVTSSPNNLNFTLNYMVRFEINGQVAFCIEP
                     GVSAAEGQVYTATALKNYLKNSNVRKKTSLISYLGYVTNKDKSDEQYIAAQFMIWEAL
                     GTKIPIKNIRVDYDRRKAEIQRLINNFGEKATFNNQTHTVKIGETLKVKNKNDFLKYV
                     KDIQKPKGVNVSIHGNEVWITVTKEAPDKAKIQLNKIKVQGAPLAYRRPGAQTIGVLH
                     PTEPNASVINLQILKQGNIRVFKEDAETGVKAQGAASLENAVYGLYQADGKKIKEITL
                     KKINGKVQAEIKDLDPGKYVLKEIKAPKGYVLSDEAIEIVVEPGKTVNATAKDQVIKG
                     TIDLVKVANKDLVDSTNPDNKPKLAGIEITLTSKTTGKVVKTVVTDNDGYATFGKNTV
                     VFDTYILSETKGKEGYKLFEPLEVTISENGQTFHYVLEDKVIEQRLKVVKVDQETGKV
                     IPLANTEFKIFDTLANRYVTMEIPNDTETTDIFKTNEKGFFLTNGTLTYGKDRYRLEE
                     IKAPVNYVLNQNPLVFSITNDTEPIKVIHFANRQARKSVKLFKYEEWNKKKTPLAGVN
                     FTLYNGKNQAIGEYTTDQNGEILVKGLVADDYYFLENAPLESFQPNTDKQAFTVAFKQ
                     NAPDDGETLVVSVRNYLIPPKLETQASNKADGEKVVDPLETIVIKDSVSYTNLFVGKE
                     YTISGVLMNKATNAPILQDGKEITSTLAFIADKVNGTKDLEFVVNASVLKGQTIVVFE
                     KLFRDGIEVAAHADINDEAQSVKVNNPTVHTTAVFEDGLTVADPTGLFTLNDTVEITE
                     ILPGKAYLVKGILMDKATGEPLLVHGNTVETSVGFIAKAENETVIVPFTFDLTGINGR
                     EIVVFESLFRNDEELANHKDINDKGQTVRVTNPSVRTTATHKPTGLKEVNPLTKVTIT
                     DAVKYHDLVVGKTYTVNGVLMDKVTEKPVLVDGKEVTSTLTFVAETTDGTVNLDFILD
                     ARALRGKEVVVFEDLFREGVLLASHADITDNDQTIKISNPTITTKATNKNGGKEILAL
                     PNQTVLEWVKVDGLAIGQIYRLIVQGYLTPDGTPFEGTHAEKVFAADKEMMEFLFEFL
                     VDGRNLAGKGLSFAEWLQAKTEDEKETFEEVAKHNVDLTNKDQTVQIVEAPKPPQPVK
                     PEQRVKPATVVPMLPKTGEQTSSILMVLGLVVLVIALGITLYKVRKAKD"
     gene            5569..5934
                     /locus_tag="ACG8KR_002365"
     CDS             5569..5934
                     /locus_tag="ACG8KR_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0201688.1"
                     /translation="MAEKALRMKKEFLGQVFLLGSREPNPPKEINGVSGRYVQLQAEC
                     SREEQPAFFVYLTAEEDKRLRLPEKEVRISFEEAYFVFRKERVNSRETNDMGQTERRT
                     TGYQDVGVFLTGVKIGEVL"
     gene            5936..6388
                     /locus_tag="ACG8KR_002366"
     CDS             5936..6388
                     /locus_tag="ACG8KR_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0201689.1"
                     /translation="MGLKFPNNYDITEALDETCLGAVRFGALEESDRVFDNETGDYTG
                     EIRSQRAQLVFGNPDEPKIARRVKFPADVDLTGFKRRDPVKLIDPEYHVVYIPAQDGN
                     REYSDLVIYAKGLEKVEAPTNNPNMKQSQEKGLDQKQPSVDSKEKEKK"
     gene            6837..8312
                     /locus_tag="ACG8KR_002367"
     CDS             6837..8312
                     /locus_tag="ACG8KR_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HFQ0201690.1"
                     /translation="MKAHFFKYRGRRIRYFNRYLWFCYYGFFLFPFLILLGWFSWKVF
                     YPRLSTFSNWQMYLIPGISIVLYLLLVSGLILGLMHWSRLKEGYFASVYWRQLLVRML
                     IDNRFVYTKKQVSEKQTREKMRLPKVYFYQKKQELVVSFPLDGGRFHERFLKMGHLLS
                     EVFLRDLIREEREKARINYVFLSDSGRIPIDQCIAENGRIHLMKTLDWVYDQSPNMLI
                     AGAIGGGKTYLLYSLIQACLSVGTVDICDGKAADLAALGDVDVFKGHVFYKINEEMII
                     CLRNALKEMNQRYAYMKTMKDPKYEPGKNYAYYGLPPHFIFFDEWAAFYGSLDMEARN
                     RVDKLIQPLVLKGRQAGMYIILAMQRPDAEYFPSGVRDNLTFRISVGRLSPIGYLMTF
                     GEDFKNKPFYNKDQPGRGYAFPGFDVPVEFFAPFVYKDYHFIKEFEKFTEMIVYDFTV
                     KEDSQDQTMGDDNQLLQEQLERQMYLAKDPKGTSFSDAHIL"
     gene            8335..>8542
                     /locus_tag="ACG8KR_002368"
     CDS             8335..>8542
                     /locus_tag="ACG8KR_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0201691.1"
                     /translation="MAESCYYRVATAIRMINPSLSSRTFYDWLNRIEQVTDYRFLRKE
                     RVFTGKVINQVLLTKKDIERLTRLY"
BASE COUNT         2956 a         1297 c         1838 g         2451 t
ORIGIN      
        1 gaaaagatag taatttccga ttattggagt ttttttattt ataaagaaga agcgaataag
       61 ttgtgattcc tagatttcgc gcgtgattaa ttaatgagca aaaaatgcgg aaagttttag
      121 tgtttaatac acgaggacaa agtttcttag gtattgaagg cacacttgta acggttgatg
      181 ggtggtgtga ttgtttcgag gttggaaaac atgttgaact tatggcattt actgagctaa
      241 aagataagaa caacgtggaa tttttcgaga gtgatattgg ttgggacgat catgaagaag
      301 tttatggaaa atttattttg cggaagggaa attcttatac gaatggggaa gcataagtag
      361 cgatttgtta gagatcattg ataggattga aattgtcggg aatatttatg agaatctaga
      421 gttatttgag gtgatagaat gagtgatggt tttgaacaag tatcaataat taaaaatcaa
      481 gttcgggaga ttcttagaaa aaaaggctat ttggtagata gttattttga aggagattac
      541 gaaacatggg taggtgtcta tgctcgtcca gagaataaac ctacctattt agatcctacg
      601 acgtcagaag atgcctattt acaaaatagg tatcgagtag atggttttaa gcaagacttt
      661 gcggagtggt ttgaatggga aattgaaaac ggagaagtaa aagaagaata agagtacttg
      721 ttgtatcaaa ataagccagt agaacaacca aaccagtagc tagtaatgac aataatactg
      781 aggaatcgga tcattatggt atgattgata tttctcttcc agaagatctt aagccagatc
      841 ctgtaacaat tggcaatagt gtcatgattt ttgattcatt agatgaagct atgtcatatg
      901 gttggaaaga aattaaggat gaaggtagta attggtatgg catgagattt ggtgatggtg
      961 aagttgccaa aaaagctggt attccaattg ggaaatggac agttgatttc cacgaagcag
     1021 gttctttttt tgttctgtaa ttatagtggg agggtaaaat cattgatgaa atgtgttcag
     1081 ataagagcgc cagataaaaa tagaagagac agttcacgtc gttatcactt ttggtaacgg
     1141 cgttttgttt tattatataa aattctcagg aaaaggagga attcttgtgt atgcaaaaac
     1201 gaaaatgaat tggttgaaag cactcgcatt ggtggctttt tctcaattct gtttcttggt
     1261 gggaactgct caagcacatg cagaagaaac gacggctata caacctacga tggaagccat
     1321 tgcccaagtc gcttctgaag tgacagaagt ccaaccagca gaagccgggg tcccagctgg
     1381 tgaagccact gttgaacagg cccctgtgac agaagcgcca acggtcaatt ccaaaacgac
     1441 tctcgatcag acgattgggg aaacacaaca cccagggaat agtcctggta cttcacaacc
     1501 agttgaagaa actgcctcaa ttacaacccc tgcttcagaa acgtccactc tagaaggaga
     1561 aaaagctgcc gaagcaacgc cttctgtgca gccagagtct tctgagaaac aagcggaaga
     1621 aaagaaacga atagaagaaa cagtggaaca accagcggct actgctgaca aagaaggaaa
     1681 aaatgaggaa aacgttgaaa aaacaatctc ttcaaccgca gctagtcaaa atcaaccaac
     1741 gaaaccaaac aaagaatcta cggctagtat tgaaaatcaa aagaaaaaag agcagaacat
     1801 tccaaaaaca ggtacaccta cactgttagt aacagtacca accaatcgtt cggttcgtgt
     1861 tcgccgtgca accaatgcag ctgttttagg tggtcggaaa attgattggg gcgaaaatat
     1921 tggtgttact tctagtccga acaatctaaa cttcacttta aattatatgg ttcgttttga
     1981 gattaatgga caagtggcgt tttgtattga gcctggtgtc tcagccgcag aaggtcaagt
     2041 ttatacagct actgctttga aaaactattt gaaaaattca aatgtacgga aaaaaacaag
     2101 cttgatctct tatttaggat atgtcacaaa taaagacaaa tccgatgaac aatacattgc
     2161 ggcacaattt atgatttggg aagctttggg cacaaaaatc ccaatcaaaa atatccgtgt
     2221 tgattatgat cgtcgaaaag cagaaattca acgtttgatt aataactttg gtgaaaaagc
     2281 aacttttaat aatcaaacac atacggtaaa aattggcgaa acgttgaaag taaagaataa
     2341 aaatgatttt ttgaaatatg tgaaagacat tcaaaaacca aaaggtgtca atgtatctat
     2401 tcacggcaat gaagtatgga ttacagtcac aaaagaggca ccggataaag caaaaattca
     2461 attgaataaa attaaagttc aaggtgcgcc attagcctat cgtcgtccag gagctcaaac
     2521 aattggtgtg ttacatccaa cagaacctaa tgcgtctgtt attaatttac aaattctaaa
     2581 acaaggaaac attcgtgtgt ttaaagaaga tgcggaaact ggtgtgaaag cacaaggtgc
     2641 agcttcttta gaaaatgcgg tctatggttt gtatcaagca gatgggaaaa agattaaaga
     2701 aattaccttg aaaaaaatta acggcaaagt acaggcagaa attaaagatc ttgatcctgg
     2761 caaatatgtt ttaaaagaaa tcaaagcgcc aaaaggttat gtgctttctg atgaagcaat
     2821 tgaaattgtt gttgaacctg gaaaaactgt caatgcgacc gcaaaagatc aagtgatcaa
     2881 aggaaccatt gatttagtga aagttgctaa taaagattta gtcgattcaa cgaatccaga
     2941 taacaaacca aaattagctg gcattgaaat cactttgacg tcaaaaacaa ctggcaaagt
     3001 ggtgaaaacc gttgtgacag acaatgatgg ttatgctact tttggtaaaa ataccgttgt
     3061 ctttgatacg tacattcttt ctgaaaccaa aggaaaagaa gggtataaac tatttgaacc
     3121 acttgaagtc acaatttctg aaaatgggca aactttccat tatgttttag aagataaagt
     3181 aattgaacaa cgtcttaaag tggtgaaagt ggatcaagaa acaggaaaag tgattccact
     3241 tgctaataca gaattcaaga tttttgatac tttagccaat cgttatgtca caatggagat
     3301 tccaaatgat acagaaacaa cggatatctt taaaaccaat gaaaaaggct ttttcctaac
     3361 taacggtaca cttacttatg gaaaagatcg ctatcgtttg gaagaaatca aagcccccgt
     3421 aaattacgtt ttgaatcaaa acccattagt gttctcgatt accaatgata cagaaccgat
     3481 caaagtgatc cattttgcga accgtcaagc acggaaaagt gtaaaattat tcaagtatga
     3541 agaatggaac aagaagaaaa cgccgctcgc aggtgtgaac tttaccttgt ataacggtaa
     3601 aaatcaagca attggtgaat ataccactga tcaaaacggg gaaattttag tgaaaggtct
     3661 agttgccgat gactattact tcttggaaaa tgccccatta gaaagtttcc aaccaaatac
     3721 agataaacaa gcattcacgg ttgcattcaa acaaaatgcg ccagatgatg gggaaacgtt
     3781 agtggtttct gtacgtaatt acttgattcc accaaaacta gaaacacaag cgtcaaataa
     3841 agccgatggt gaaaaagtgg ttgatccatt agaaacgatc gtcattaaag acagtgtttc
     3901 ttataccaac ttgtttgtcg gaaaagaata caccatttca ggagtattga tgaacaaggc
     3961 aacgaatgcg ccaattttac aagatggcaa agaaatcaca agtaccttgg cctttattgc
     4021 agacaaagtc aatggcacaa aagaccttga atttgtggtt aatgctagtg ttctgaaagg
     4081 tcaaacgatc gttgtttttg aaaaactttt ccgtgatggc attgaagttg cagcacatgc
     4141 ggatatcaac gatgaagccc aaagtgtgaa agtgaataat ccaaccgttc ataccacggc
     4201 tgtctttgaa gacggattga cagtcgcaga cccaacagga ttgtttacct taaacgatac
     4261 cgttgaaatt accgagattc ttccaggaaa agcatacttg gtcaaaggga tcttaatgga
     4321 taaagcaacc ggtgaaccct tgcttgtgca tggaaatacg gtagaaacga gtgttggctt
     4381 tattgccaaa gcagaaaatg aaacggtgat cgtaccattt acctttgatt tgacaggtat
     4441 aaatggtcgt gaaatcgtcg tgtttgaaag tctttttaga aatgatgaag aactagcaaa
     4501 ccataaagac atcaacgaca aagggcaaac ggtacgagta actaacccaa gtgtacgtac
     4561 aacggctaca cataaaccca ctggcttgaa agaggttaat ccattaacga aagtcaccat
     4621 tacagacgct gtgaaatacc acgatttggt cgttggaaaa acatacacag tcaacggtgt
     4681 tctaatggac aaagtaacgg aaaaaccagt gctagtagat ggaaaagaag tcacaagtac
     4741 cttaacgttt gtggcagaaa caacggatgg tacagtcaat ctagacttta ttctagacgc
     4801 acgtgcgttg cgaggcaaag aagtcgtggt ctttgaagat ctgtttagag aaggcgtatt
     4861 attggcttct cacgcagata ttaccgacaa tgaccaaacc atcaaaatca gtaatccaac
     4921 cattacgacg aaagcgacga ataaaaatgg cggaaaagag atcttggcct tgccaaatca
     4981 aactgtgctt gaatgggtca aagtagacgg tcttgcgatt ggtcaaatct atcgcttaat
     5041 tgttcaaggc tacttaacac cagacggcac accatttgaa ggcactcatg cggaaaaagt
     5101 ctttgcggcc gataaagaaa tgatggaatt cttatttgaa ttcttagtgg acggtcgcaa
     5161 tcttgcagga aaaggcttga gttttgcgga atggctccaa gcgaaaacgg aagatgagaa
     5221 agaaacgttt gaagaagtgg cgaaacacaa tgtagatcta acaaacaaag atcaaacggt
     5281 tcaaattgtg gaagcgccaa aaccaccgca accagtgaaa ccagaacaac gggttaaacc
     5341 agctactgtt gtaccaatgt tgccaaaaac aggtgaacaa acaagtagta tacttatggt
     5401 tcttggatta gtagtattag tcattgcttt aggtataaca ctttataaag tacgtaaagc
     5461 caaagattag tcatgaggaa caagcggtta ggatttttaa ccgcttgttt gattgtaatg
     5521 tagaaatatt tagaatttat tttggttttt aagaggagga aaaactttat ggcagaaaaa
     5581 gcattgcgta tgaagaaaga gtttttaggt caagtatttt tattgggttc acgggaacct
     5641 aatccaccaa aggaaattaa tggtgtgtct gggcgatatg ttcaattaca agcagagtgt
     5701 tcgcgagaag aacagcctgc tttttttgtt tatctcacag ctgaagagga taaaagatta
     5761 cgattaccag aaaaagaggt gcgtatttca tttgaagaag cttattttgt ttttcgtaaa
     5821 gaacgagtga atagtcgtga aacaaatgat atgggacaaa cagagcgtcg aacaactggc
     5881 tatcaagatg ttggtgtctt tttaactggt gtaaaaattg gggaggtttt gtaagatggg
     5941 attaaaattt ccaaataatt atgacattac agaagcatta gatgaaactt gtttaggagc
     6001 cgtacgcttt ggcgcattag aagagtctga tcgtgtattt gataatgaaa caggcgatta
     6061 tacgggagaa attcgatcac agcgagcaca attagttttt ggaaatccag atgaaccaaa
     6121 gattgcacga cgagttaaat ttccagcaga tgtagatttg actggtttta aacgacgtga
     6181 tcctgtgaaa ttaattgatc cagaatatca tgtggtatat attccagcac aagatggcaa
     6241 tcgtgaatat tcagatttag tgatttatgc gaaaggatta gagaaagtag aagctccaac
     6301 aaataatcct aatatgaaac aatcacaaga aaaaggttta gatcaaaaac aaccaagtgt
     6361 tgattctaag gaaaaagaga aaaaataagg tttttttgaa agaataggaa ggagtcttcc
     6421 tattcttttt atctaagata gaaggaaggg gagattttga attcagataa aaccaatcaa
     6481 atactttgag agattgtgct tgttatcaaa atatccaaca attgattgag tattttggag
     6541 acaatccaac gatttttgta caaacaaatg catatatttg tgcggttatt gggcagtgta
     6601 tgtttttacg tgatcttgtt cgtttttcat taccgtacca gtggaaacaa gatcatcagg
     6661 aagtgatttg gattcgtata gtacgaatat gtaatatgtg tgcacaccct tataaaaatc
     6721 gattaaatct ttttcgatta tgttttttta ctaaatgtga tattcctgta ttagttgctt
     6781 tttgtcaaaa gtttttagca caggaaaagt aaaaacagaa aggaggaatg tgacaattga
     6841 aagctcattt tttcaagtat cgtggtcggc gaattcgcta tttcaatcgt tatttatggt
     6901 tttgctatta tggtttcttt ttgtttccat ttttgattct gttaggttgg tttagttgga
     6961 aagtatttta tccacgttta agtacgtttt cgaattggca aatgtatctt attcctggaa
     7021 taagtattgt gttgtatttg ttgctggtta gtgggttaat tttagggttg atgcattggt
     7081 cgcgactaaa agaaggatat tttgcctctg tttattggcg tcagttactt gttcgaatgt
     7141 taattgacaa tcggtttgtt tatacaaaga aacaagttag tgagaaacaa acaagggaaa
     7201 aaatgagatt accaaaagtt tatttttatc aaaagaaaca agaattagtg gtttcttttc
     7261 ctttagatgg tgggcgtttt cacgaacgat ttttaaaaat gggtcatttg ttatctgaag
     7321 tatttttgcg tgatttaatt cgtgaagaac gagaaaaagc ccgaatcaat tatgtattcc
     7381 tttcagattc tggtcggatt cctattgatc aatgtatagc agaaaatgga cgtattcatt
     7441 taatgaaaac tttggattgg gtatatgatc aatcaccaaa tatgttgatt gctggtgcaa
     7501 tcggtggtgg gaaaacctat ttattgtatt ctttaattca agcttgttta agtgttggga
     7561 cagtagatat ttgcgatgga aaagcggcag atttagcagc attaggtgac gtcgatgtat
     7621 tcaaaggaca tgtgttttac aaaataaatg aagaaatgat tatttgttta cgaaatgcat
     7681 taaaagaaat gaatcaacgg tatgcctata tgaaaaccat gaaagatcct aaatatgaac
     7741 cagggaaaaa ttatgcttat tatgggttac caccgcattt tatctttttt gatgaatggg
     7801 cagcttttta tggttcgttg gatatggaag cgagaaatag agtcgataaa ttgattcagc
     7861 cattagtatt aaagggtcgt caagcaggga tgtacattat tttagcaatg caacgaccag
     7921 atgcagaata ttttccaagt ggtgttcggg ataatttaac attccgtatt tctgttggtc
     7981 gattgtctcc cattggttat ttaatgactt ttggagaaga ttttaaaaat aagccatttt
     8041 acaacaaaga tcaacctggt cgtggatatg cttttccagg atttgatgta cctgtagagt
     8101 tttttgcgcc atttgtttac aaagattatc actttatcaa agaatttgag aaatttactg
     8161 aaatgattgt ctatgatttt acagtaaaag aggatagtca agatcaaacg atgggagatg
     8221 ataatcagtt gttacaagaa caactagagc gccaaatgta tttagcaaaa gatccaaaag
     8281 gaacaagttt ttctgatgcg catattttgt aatcaactaa ggaggtgaag ttaaatggca
     8341 gagagttgtt attatagagt agctacagca atacgaatga ttaatccttc tctttcttcc
     8401 agaacgtttt atgattggtt gaatcgtatt gaacaagtaa ctgattaccg ctttttgcgc
     8461 aaagaacgtg tttttacagg aaaagttatt aatcaagtat tgctaacgaa aaaagatata
     8521 gaacgattga ctcgattgta tc
//
LOCUS       DAZKLD010000125         8390 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.125, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000125 DAZKLD010000000
VERSION     DAZKLD010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8390
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.125"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(35..328)
                     /locus_tag="ACG8KR_002369"
     CDS             complement(35..328)
                     /locus_tag="ACG8KR_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HesB/YadR/YfhF family protein"
                     /protein_id="HFQ0201692.1"
                     /translation="MKLTVTPKAQKWFDSEIDLPETYGIRFFGKVYGKTEVHDGFSIG
                     MSVEQPEHPVKKVEMDGTLFFIEEADEWFFKGYDLLVDYDPNLEEPTYHFKKQ"
     gene            complement(356..1021)
                     /locus_tag="ACG8KR_002370"
     CDS             complement(356..1021)
                     /locus_tag="ACG8KR_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HFQ0201693.1"
                     /translation="MKQNFAIVGLGRFGGSICRTLIEAGQEVLAIDSNEDRVNEYMNI
                     ATHAVVANAQDEMTLRSLGIRNFDHVIVAIGEDIQASILVTLMVKEMGVPNILAKAQN
                     EYHARVLEKIGADRVVHPERDMGIRIAHNLVSKNILDYLELSDKYSLAEIRVSNPKFF
                     NKTLAELNFRQRFDLTVVAIRRVDQTVIASPAADEYVRENDNLLVIGETGDVDILDDK
                     MNQ"
     gene            complement(1161..1793)
                     /locus_tag="ACG8KR_002371"
     CDS             complement(1161..1793)
                     /locus_tag="ACG8KR_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFQ0201694.1"
                     /translation="MIKVLLVDDHEMVRLGVSSYLSIQEDIEVIGEAENGRQGYEKAM
                     ALRPDVILMDLVMEEMDGIESTKAILKDWPKAKIIIVTSFIDDEKVYPAIEAGAAGYL
                     LKTSTAHEIADAIRATQRGERVLEPEVTTKMMEKMSRRNDPVLHEELTNRENEILMLI
                     SEGKSNQEIADELFITLKTVKTHVSNILAKLEVEDRTQAAIYAFKHGLVK"
     gene            complement(1800..2867)
                     /gene="liaS"
                     /locus_tag="ACG8KR_002372"
     CDS             complement(1800..2867)
                     /gene="liaS"
                     /locus_tag="ACG8KR_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     LiaS"
                     /protein_id="HFQ0201695.1"
                     /translation="MMGRISKAMLAVYSGIAAFLIILFSLFTYFYASNQSHWWGELLR
                     ARLLYVPLIFHLMAISLGVGLIVFLLLSLIQKAKYGKIEEKLRALSSGNYESKLLHQP
                     IPSASDDLYIKDIDMEITKIKEKMIEVSSELQIMTSRPQYVDGQTKEEILKLERHRLA
                     RELHDSVSQQLFAAMMMMSALTEQAEKSETPEMFRKQLKMVAEIINASQSEMRALLLH
                     LRPVNLEEKSLKQGIEQLLKELQNKIQISLKWEVEDVKLTSSIEDHLFRIVQELLSNT
                     LRHAKANELEVYLHKIDNNLLLRIIDDGTGFNMNETKTGSYGLNNIKERVAGIGGTVK
                     IISFKGQGTSVETKVPLMKEA"
     gene            complement(2864..3595)
                     /gene="liaF"
                     /locus_tag="ACG8KR_002373"
     CDS             complement(2864..3595)
                     /gene="liaF"
                     /locus_tag="ACG8KR_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein
                     LiaF"
                     /protein_id="HFQ0201696.1"
                     /translation="MNSSWRFFVVVEALLLLFAVWQIVNNTGLLLLVIFGIFNIYLAM
                     RKYPRTKFQNFQLILGSLVIFFSLVNSPALWMMVVLAVLFIGLKGVEISGIDLTKNAF
                     WRKKQIMMVQTEQLKTHNNERKRQQLFGNQRIGNDVYEWDDINIAIISGDTIIDLGNT
                     LLPKDDNIVIVRKGIGRTRILVPLGVAIRLEHATLVGNVLFEEEQFSLKNEQIKICSS
                     DYDENPRRLKIITNTLVGDIEVIRI"
     gene            complement(3763..4242)
                     /gene="greA"
                     /locus_tag="ACG8KR_002374"
     CDS             complement(3763..4242)
                     /gene="greA"
                     /locus_tag="ACG8KR_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HFQ0201697.1"
                     /translation="MVEKVYPMTLEGKEKLEKELEELKTVKRGEIIERIKIARGFGDL
                     SENSEYESAKDEQAFVEGRITTIENMIRFAQIIDNDGVDADEVSIGKTVTFIELPDGD
                     EEEYTIVGSAEADPFSGKISNDSPIAQALIGKRKNDEVTIATPGGDMLVKIVKVEQA"
     gene            complement(4378..5553)
                     /gene="mltG"
                     /locus_tag="ACG8KR_002375"
     CDS             complement(4378..5553)
                     /gene="mltG"
                     /locus_tag="ACG8KR_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HFQ0201698.1"
                     /translation="MSGNKQHPSKDKKEASKAATADIIDKRRNLRKKEDKIVRKIILV
                     IALTLLIIGGFLGFTVYRYVDSGLKPLDKSDDQLVQVEIPSGSSNKQIGEILEKDNII
                     KSGIVFNYYTKFKNLTGFQAGYYQLAPNMTLDEIGKQLQEGGTSEPTKVADGKIAIPE
                     GYDIDQIAERVAKVTGKDKKEFLDLVNDETFFNELYQKFPELLESASKAENTKYRLEG
                     YLFPATYDYYTSTSLKDLVIEMVNKTNTVMQNYYSAIKQKNLTVQEVLTLASLVEKEG
                     VKENDRKNIAQVFFNRIKANMPLQSDISVLYALGEHKELVTYEDTAVDSPYNLYTNTG
                     YGPGPFDNPSEEAIKAVLEPAENDYYYFVADTSTGNVYFAKTYEEHMELVQKYVNNS"
     gene            5718..6878
                     /locus_tag="ACG8KR_002376"
     CDS             5718..6878
                     /locus_tag="ACG8KR_002376"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HFQ0201699.1"
                     /translation="MAKGKHQKKQQISLSFLAVLLLAVSFFSTGIITGWQLTKQPQPQ
                     IYKTEPAQLASNLTSNEQMLASKIDQLLQKSDYIGSIYVRKNNRVILEKGYGYANLRI
                     KESNSPSLYYQIGSIQKAMTALLILKQIQSGKITFDTKLSEFYPQIPGSQQISIKNML
                     YMRSGLHRTASPKKPMSDSEIIQFALHHLKFTGYDTYHYEPLNYTLLTGVLIKLMNQP
                     YETLLKKEIIRPLHLEHTDFYENVKNSPQHAVSYQMSATDDYSKALVEPETDIRNELG
                     TGNISMSVYDLNKFFTSVLSGDIIPKELLFSLWQNNGDKHPYSGGVYSGNDYILAQGN
                     INRFHAVAAMKKDTKDAIVMESNIQSDKNIKLPATDLRNQIYELMEGVNLKS"
     gene            complement(6918..7865)
                     /locus_tag="ACG8KR_002377"
     CDS             complement(6918..7865)
                     /locus_tag="ACG8KR_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFQ0201700.1"
                     /translation="MLNYLITGGAGFIGSTLANYLGKENNVVVIDDLSMGKKENLDES
                     KHITFIEGDVADKNLMEKVMKEYHFDYIFHLAAVASVADSVERPLETHRVNFDSALLL
                     LELVRKYQSGLKRLVFSSSAAVYGDEPTLPKKEESVIRPLTPYAIDKFAAEQYVLDYC
                     HLYDVPTSAVRFFNVYGPNQNPNSPYSGVISILVDRYKKQLAGEKTEFTLFGDGSQSR
                     DFVYIEDVIQALLLVAKEEKALGQQFNVGTGKSTTLLELIHSIDQILGTELALKYEAE
                     RSGDIRDSLADISKIRSLGYQPKFDILNGMERYLKTEID"
     gene            complement(7898..>8390)
                     /locus_tag="ACG8KR_002378"
     CDS             complement(7898..>8390)
                     /locus_tag="ACG8KR_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008754 - O antigen ligase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HFQ0201701.1"
                     /translation="HINNTQTPDNTLVRKDVAENTDISNARITIWKSAWEIFKTSWLI
                     GVSPKNVVSYAQSIIPDGYIAKVGIAIHNAYLNVLTSTGILGIIPFMAFLIKSFVLNV
                     YRIIYNKCIGKDYHFVYCLVILSYALYAALNNELVYENTVGTFVFWLFLGRVNSKNNS
                     SYF"
BASE COUNT         2505 a         1751 c         1309 g         2825 t
ORIGIN      
        1 gaaggtgaca aaaattgtgt caccctctct ttttttattg ttttttaaag tgataggttg
       61 gctcctccag gttgggatca taatcaacaa gtaaatcata tcctttaaag aaccattcat
      121 ctgcttcctc gataaaaaaa agtgtcccat ccatttccac tttctttaca ggatgttctg
      181 gctgttcaac tgacattcca atcgaaaaac catcatgaac ctctgttttg ccgtagactt
      241 ttccaaaaaa acgtattccg taagtttctg gcagatcaat ttctgagtca aaccactttt
      301 gtgcttttgg cgttaccgtt aatttcatcc caacaaccta ctttctcttt tctttctatt
      361 gattcatctt atcgtctaga atatccacat cgcctgtctc tccaataacc agtagattat
      421 cattttcacg aacatattca tctgctgcag gagaagcgat aaccgtttga tctactcttc
      481 gtatggcgac tactgttaag tcaaaacgtt gcctgaaatt taattcagct aatgttttgt
      541 tgaaaaattt aggattggag actcggattt ctgctaaaga atatttatct gacagctcta
      601 agtagtctag aatattcttt gatactaagt tatgtgcaat ccgtatcccc atgtctcgct
      661 cagggtgtac gacacgatct gctccgatct tctctagtac acgggcatgg tactcatttt
      721 gggcttttgc taaaatattc ggtacgccca tttcctttac catcagagta actaagatgc
      781 tggcttggat atcttctcca atagcgacaa ttacatgatc gaaatttctt atacctaatg
      841 aacgaagtgt catctcatcc tgtgcatttg ccacaacagc atgggtggct atattcatat
      901 attcattgac tcgatcttca ttactgtcaa tagccagtac ttcttgtcca gcttcgatca
      961 gtgtacggca gatgcttccg ccaaatcgtc caaggcctac aattgcaaaa ttttgtttca
     1021 ccattttcct cctttagtat cttcttttct cagtataaca aagcctttcc aaaaacaaaa
     1081 gcaagcatca ttattgtaaa aaagccaaga aaaataatag ttattttctc agcttttgat
     1141 tatcctcttt atcatcgttt ttatttcacc aaaccatgtt taaacgcata gatagctgct
     1201 tgagttcggt cttctacctc taacttagct aatatattcg acacatgcgt tttgactgtt
     1261 ttcagtgtga taaacaattc atccgcgatt tcctgattac ttttcccttc agaaatcagc
     1321 atcaaaattt cattttctcg attcgttaat tcttcatgca atactggatc atttcgccga
     1381 ctcatttttt ccatcatttt tgttgtcact tcaggttcta gtactcgctc gccgcgttgc
     1441 gtagccctta tagcatcagc tatttcatgt gctgtagacg tcttcaacaa atatcctgct
     1501 gctcctgctt caattgcagg ataaactttt tcatcatcaa taaaacttgt gacgataatg
     1561 attttggctt ttggccaatc ttttaagatt gcttttgttg attcgatgcc atccatttct
     1621 tccatgacta gatccattaa aatgacatcc ggtcgaagtg ccatcgcctt ttcatagcct
     1681 tgacgtccat tttcagcttc tccaatcact tcaatgtctt cttggataga taaataagaa
     1741 gagacgccta agcgtaccat ttcatggtca tctactaata aaacttttat catctttatc
     1801 tatgcctcct tcatcaaagg gaccttggtc tccacgctcg taccttgccc tttaaaacta
     1861 atgattttta ctgttccacc aataccagct actcgttctt tgatattgtt caatccataa
     1921 cttcccgttt tggtttcatt catattaaat cctgtcccgt cgtcaatgat acgtaataaa
     1981 agattgttat ctattttatg caaatagact tctaattcat tagccttagc atgtcttaat
     2041 gtgtttgata acaattcttg aacgattcgg aacaagtgat cctcaatgga actagttaat
     2101 tttacatctt ctacctccca ttttagcgaa atctggattt tattctgcaa ttccttcaat
     2161 aactgctcaa tgccttgttt taaacttttt tcttctaaat tgactggacg cagatgaaga
     2221 agcagcgcac gcatctcaga ctgggaagcg ttgatgatct ctgccaccat tttcaactgc
     2281 ttacggaaca tttctggtgt ctcacttttt tctgcttgct ccgtcaatgc cgacatcatc
     2341 atcatcgctg caaataattg ctgtgaaact gaatcatgaa gctcacgagc taatcgatgc
     2401 ctttctaatt ttaaaatctc ttctttggtt tgtccatcta catattgagg acggctagtc
     2461 ataatctgta attcactgga tacttcgatc attttttctt ttattttagt aatttccata
     2521 tcaatatctt tgatgtacag atcatccgac gcgcttggga tcggctgatg caaaagtttg
     2581 gattcataat tgccagaaga aagtgcacga agcttttctt cgattttccc gtattttgcc
     2641 ttttgaataa gtgataacaa tagaaatacg atcaatccta cacctaatga aattgccatc
     2701 aaatggaaga taagcggaac gtataataaa cgcgcacgca gcaattctcc ccaccaatga
     2761 ctttggttac tggcataaaa ataagtgaaa agtgaaaata gaataataag gaaagccgca
     2821 atccccgaat aaacagctag cattgctttg gatattcttc ccatcatatt cgtatcacct
     2881 cgatatctcc aaccaaagta ttggtaatga tcttcaatct gcgaggattt tcatcataat
     2941 cactgctgca gattttgatc tgttcgtttt ttaaggaaaa ctgctcttct tcaaacaaga
     3001 catttcctac taatgtcgca tgctctaaac gaatagccac tcccaggggt actaagatcc
     3061 gtgttctgcc tatccctttt cttacaatca caatattatc atcttttggc aaaagtgtat
     3121 tgcccaaatc gatgatagta tcaccagaaa tgatggcaat gttgatatca tcccattcat
     3181 agacgtcgtt tccaatacgt tggttaccaa ataattgctg tcttttacgc tcattattat
     3241 gtgtttttaa ttgctctgtt tggaccatca tgatctgttt ttttcgccaa aaagcatttt
     3301 ttgtcaaatc tattcctgaa atctctacac ctttcagccc gataaataaa acggccaata
     3361 caaccatcat ccataaagca ggactgttga ctaagctaaa aaaaatgacc aagctcccta
     3421 agatcaattg aaaattttga aatttagtac gaggatattt gcgcatagct agatatatat
     3481 tgaaaatacc gaatattact aaaagcaaaa gtccagtatt gtttacgatt tgccaaacag
     3541 caaataacag cagcagtgct tcgactacca caaaaaaacg ccaagaacta ttcatcgctt
     3601 tttctccctc ctgacacatt ctcatttcat catacctatt tttttgactg ttggctatag
     3661 gactttagat gtaagatcgt tcagtgaaac ctgcaatact caaagagaaa agatctagag
     3721 tgcgtcatca gtaagatgaa ctctctagat ctttcagtca gactacgctt gttctacttt
     3781 gacgatttta actaacatat cgccacctgg tgtagcaatg gttacttcat catttttacg
     3841 tttgccaatc aatgcttgcg cgataggaga atcattggaa atctttccag aaaacgggtc
     3901 tgcttcagca cttccgacaa tcgtgtactc ttcttcatct ccatcaggca actcgatgaa
     3961 cgtaactgtt ttaccgatcg atacctcatc tgcatctacg ccgtcattat cgatgatttg
     4021 tgcaaaacgg atcatatttt caattgttgt gattcttcct tcaacaaatg cttgttcatc
     4081 tttagctgat tcgtattcgg agttttcgga aagatctccg aaaccgcgag cgattttgat
     4141 tcgttcaatg atttctccgc gtttaactgt ttttagttct tctaattctt tttctaattt
     4201 ttctttacct tctagagtca ttggataaac tttctctacc atgtgtaacg tctccttttt
     4261 tcgataaaat accgcaaaag gtaatctttt gaggaatata gtccaaaaaa gattaccacg
     4321 cttttcttaa aatgtaaatg tttttctaaa aatgatcaat gactagcccc tgtttttcta
     4381 agaattattt acatattttt gcactagttc catatgttct tcatacgttt tagcgaaata
     4441 aacatttcca gtgctcgtat ctgctacgaa ataataatag tcattttctg ctggttctaa
     4501 tactgccttg attgcctctt cgcttggatt gtcaaaagga ccaggcccat agccggtatt
     4561 ggtatacaaa ttgtatggtg aatctacagc tgtatcttca tacgtcacca attctttatg
     4621 ttctcctaat gcatataaaa cagagatatc tgactgaagt ggcatatttg ccttgattcg
     4681 gttaaagaag acttgtgcaa tatttttacg atcattttct tttacgcctt ctttttcaac
     4741 tagagaagcc aaagttaaaa cttcttgtac agtcaaattt ttctgtttga tagctgaata
     4801 atagttttgc atgaccgtat ttgttttgtt caccatctca atcacaagat ccttaagcga
     4861 agtactcgtg tagtaatcat aagtagctgg gaataagtag ccttccaaac ggtattttgt
     4921 gttctcagct ttagaggcac tttccaacaa ttctgggaat ttttgataca actcgttaaa
     4981 gaaagtctca tcatttacta aatctaaaaa ttctttcttg tctttaccag ttaccttcgc
     5041 tacccgctca gcaatctgat cgatgtcgta gccttctgga atggcaattt tcccatctgc
     5101 aacttttgtt ggctcagatg tgccgccttc ttgcaactgc ttgccaatct catcaagagt
     5161 catattaggc gctaactgat aataaccagc ttgaaagcca gttaagtttt taaattttgt
     5221 ataataattg aaaacaatcc cacttttaat aatattgtct ttctccaaaa tttcgccaat
     5281 ctgcttatta gaagagccgc ttgggatttc cacttggact aattggtcgt ccgatttatc
     5341 taaaggcttc aacccgctat ctacataacg atatacggta aacccgagaa atccgccaat
     5401 aatcaataat gtaagagcga tcactaaaat gatcttcctc acgatcttat cttctttttt
     5461 acgcagattt ctccgcttgt cgattatatc tgctgtagct gccttactgg cttctttttt
     5521 gtctttgctt gggtgttgtt tgtttcctga caaaacaact cctccttact atcatcttcg
     5581 taactctgtt tattatacat gaaatttcta ctgccgtgaa ttattttacc aaatattatg
     5641 tcaaaattgt aagtggcaaa tgctcttttt gaggattaca atgaaaaata gttaaaaatg
     5701 gataggaagg atttaatatg gcaaaaggaa aacatcaaaa aaaacagcag atctctcttt
     5761 cttttctcgc agttttgttg ctggctgtca gcttcttttc aacaggcatc attacaggat
     5821 ggcaattaac aaaacaacct caaccacaaa tttataaaac ggaacccgca caacttgctt
     5881 ccaatttaac atctaatgag cagatgcttg ccagcaaaat tgatcaatta ctgcaaaaat
     5941 cagattatat cggttcgatt tatgtacgca aaaataatcg agtgatatta gaaaaaggat
     6001 atgggtacgc aaatctacgc ataaaagaat cgaattctcc ttctctttac tatcaaatcg
     6061 gttcgatcca aaaagcgatg actgctttac ttatcctaaa acaaattcaa tctggcaaga
     6121 taacttttga cacaaagcta tctgaatttt atcctcagat tcctggaagt cagcaaatct
     6181 caatcaaaaa tatgctttac atgcgctctg gtcttcatcg aaccgcttct ccaaaaaaac
     6241 ctatgtcaga tagtgagatc attcagtttg cccttcatca tttgaaattt actggttatg
     6301 atacgtatca ttatgaacca ttaaattata cattgcttac aggggtattg atcaaactaa
     6361 tgaatcagcc ttatgaaact cttttaaaaa aagaaatcat tcggccactc catctagaac
     6421 atacagattt ttatgaaaat gtaaaaaatt cccctcagca tgctgtatct taccaaatgt
     6481 ctgcaactga cgattattca aaagcattgg tcgagcctga aacagacatc cgaaatgaat
     6541 taggcacagg aaatataagt atgtctgttt atgacttgaa caaattcttt accagtgttc
     6601 tgtctggcga tattatacca aaagaactac ttttctcatt gtggcaaaat aatggtgata
     6661 aacatcctta cagcggcggt gtttacagtg gaaatgatta tatccttgct cagggaaata
     6721 tcaatcgatt ccatgcagta gcggcgatga aaaaagacac aaaagatgca atcgtcatgg
     6781 aaagtaatat ccaatctgat aaaaacatca aattgccggc cactgattta agaaatcaga
     6841 tttacgaact gatggaaggt gttaatttaa aaagctaagt cgagacaaaa aaaccagcag
     6901 gatgctggtt ttttgtttca atcgatttct gtctttaaat aacgttccat tccgttcaat
     6961 atatcaaatt tgggttgata acctaaagaa cggatctttg aaatatccgc taaagaatca
     7021 cgaatatccc ctgaacgctc cgcttcatat ttaagcgcta gctctgttcc taagatttga
     7081 tcaattgaat gaatcaattc aagtagcgta gtgctttttc ctgtaccgac attgaattgc
     7141 tgtcctaaag ctttttcttc cttagcaaca agtaaaagtg cttgtataac atcttctata
     7201 taaacgaagt ctctagattg gctaccatca ccaaataaag taaattctgt tttttctcct
     7261 gccaattgtt ttttgtatcg gtccactaga atcgaaataa ctccgctata tggagagtta
     7321 ggattttgat ttggaccata aacgttaaaa aatcggacag cactagtcgg aacatcatat
     7381 aaatgacagt aatccaaaac atattgttct gctgcaaact tatcgatggc atatggtgtt
     7441 aaaggacgga tgaccgattc ttcttttttt ggcaacgttg gttcatcgcc ataaactgca
     7501 gctgatgaac taaataccaa tcgttttaaa ccagattgat attttcgtac caattcaagc
     7561 aataaaagcg cactgtcaaa atttacacga tgagtttcta gaggacgttc tacagaatct
     7621 gccacactag ctacagcagc tagatggaaa atataatcga aatggtattc tttcatgacc
     7681 ttttccatca aatttttgtc agcgacatcc ccttcaataa acgtgatgtg cttcgattca
     7741 tccagattct cttttttccc catcgataaa tcatcgatca caactacatt gttttctttt
     7801 cctagataat tcgcgagcgt cgagccaatg aagccggcgc caccggtaat caagtaattc
     7861 aacatttttt ctcctctttt caagataaaa tttattacta aaaatagcta gaattatttt
     7921 tgctattaac tcttcctaaa aatagccaaa aaacaaacgt tcctacagtg ttttcataga
     7981 ctaattcatt gttaagagct gcgtatagtg cataagataa aataactaaa cagtaaacaa
     8041 aatgataatc tttaccaatg catttattat agatgattcg ataaacattc agcacaaaac
     8101 tctttattaa aaaagccatg aaaggaatta ttcctagaat acctgtcgat gttaatacat
     8161 tcaagtatgc attatgaatg gcaattccta ctttcgcaat atatccatct ggaataattg
     8221 actgtgcata agatactaca tttttcggag agactccgat aagccaactt gttttaaata
     8281 tttcccatgc acttttccat atagtaattc tagcattaga aatatctgta ttttcagcta
     8341 cgtctttcct aacaagagta ttatcaggtg tttgagtatt attaatatgt
//
LOCUS       DAZKLD010000126         8323 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.126, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000126 DAZKLD010000000
VERSION     DAZKLD010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8323
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.126"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(61..276)
                     /locus_tag="ACG8KR_002379"
     CDS             complement(61..276)
                     /locus_tag="ACG8KR_002379"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0201702.1"
                     /translation="MRELTLSSKELEKKTALLNEIFSADCIIQSLKESSFSKLTDQEK
                     ISYMLSLDNLGTKYDESASSDKLNRLH"
     gene            complement(327..1955)
                     /locus_tag="ACG8KR_002380"
     CDS             complement(327..1955)
                     /locus_tag="ACG8KR_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0201703.1"
                     /translation="MPEIEGIFSPKIGKKQLDFSSFPSVNRDYNLGDEVQILNIDFKE
                     LDFAVAVSNKPKALERVKKIYQENDQNIKNMIKKYRRDNSKVEDVLFNGTAKHDLNVY
                     MALGVALYCNSINQTDILVNIVRNSYQKILKISSETNPYYAMKYLGDNLLNDKKLMHN
                     TEELLSNLAAINFLFKTGSEDYFIDNVLKGAYGTAFYPLYNMKQHKAEQKDNKLEIKK
                     MKELLGIGFVKPNSKVTIASLATTNNDGKIVNGFDDSDIDILSKSYFSLYGDFADLMG
                     IDSYLVNNFEIKGSELNDIVYFVLISLYAIDQNTEYRQIDYLLVFYSLLYQFVLTRLF
                     KNAKEELILNSDYEIAKQIASIRKENKSEVIKLRKELSIASKENGRLESLVKSAKKEE
                     ELLKQEIKELKGIRKELEQLRFENKVLKQLQEQEHRQSLNGKTEEPSLSEMIAYLKHK
                     NILLVGGHVNFQNKLTELLPNIKMFDVSENNGKILKNLNTCDAIFLYTDYLNHGLYYR
                     VMSEISNKDKIFYLDNHVNMNLTIKRIYDLLSLI"
     gene            complement(1975..2178)
                     /locus_tag="ACG8KR_002381"
     CDS             complement(1975..2178)
                     /locus_tag="ACG8KR_002381"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0201704.1"
                     /translation="MSEGTEKYTIADMVNEVETLLASFFENGHVNDEWRKEEPKRVNA
                     YIARLKRFIDKYKENSDKFSKDK"
     gene            complement(2179..2868)
                     /locus_tag="ACG8KR_002382"
     CDS             complement(2179..2868)
                     /locus_tag="ACG8KR_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein"
                     /protein_id="HFQ0201705.1"
                     /translation="MRTHYIYLEDKIVDEIPAKSREQARKLFIEKYKEINGDSEVPKV
                     VVTVRKRQTKKRSTFHVSPDIYNLIERHRSEDNISISQYIATVIDELISGKYDYAFDF
                     EGEKDYKSVAVFQSDFEKLKNYAGRRNVNFARLLEEFYSEELGVGNEYKRETYAKLSH
                     KLSQALKEVTEVKAELDAKLEEVKKYQTERKNNMKNQVEFDEQKEFEKARKRYNKIMN
                     ELSYEHLTVHE"
     gene            complement(3123..3443)
                     /locus_tag="ACG8KR_002383"
     CDS             complement(3123..3443)
                     /locus_tag="ACG8KR_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0201706.1"
                     /translation="MKKKIVEGNVGVIILKDSTDYAKSMALKDEKVARMDKDLVEFLM
                     DDTERLKGDQFLSLMKDKGLNTYGLFDYKKLAIEWVKEGEIFFIEEWEGTENLVLHQY
                     LLKA"
     gene            complement(3476..4594)
                     /locus_tag="ACG8KR_002384"
     CDS             complement(3476..4594)
                     /locus_tag="ACG8KR_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0201707.1"
                     /translation="MGNNSIYTPEMQNKIKRLVGLGWMTRGKLDSFSTIKYLLMDIYR
                     QNKQTGIHFLGYDKPEDAPFFVPSSAYRASTSGSMYNLDLFEEGAKMIEYDFNEAYSR
                     IMRNYPLPSNTYLKAKYNTDQAILRAINRPKVRPYENLKTYMFFLVNFEGTAKEGTYA
                     NPKSQSMFADYGENPKTANEPIWLSEIELTLILDYYDIKEMEVLDSHTFLTKKGLLKE
                     YFERIEPLKTDPDTLKLYKNLRTKVFGAIGQKSLREGETSTFDKYSMYNRAYSSMVAG
                     VFRDRMVRYEQKYVHSAYGLLKIRTDGLYFRSEVPEFEELYKLGVVKKKVHTISKEEV
                     EKALEFKNKGFENKGSFGKSKDEVKGVVIDDDFEFDFD"
     gene            complement(4623..5417)
                     /locus_tag="ACG8KR_002385"
     CDS             complement(4623..5417)
                     /locus_tag="ACG8KR_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0201708.1"
                     /translation="MVAKKNTLKEASEKYQDMKRRGLIPKDKKGIWREFVKEVSSDRS
                     GGDFVGRYTAKGYSRRLRITVEYGTNESATELKQRYLDTRREASKKYDDFKSRGLIPK
                     KKKGIWREFFKEEDSNRNKGDFIGRYTAKGYTKNLGIKVEYGTGESSWELRNRYLNTL
                     LEKYKAELLELFDVNDFIEMAEKYGHEEGFMINDIMVIDNFTDLLALAKKARDLRETQ
                     MYLFRNKNGRNRRTESSEIEDYYDAFTHDLGAFVDEISGVMNKYRV"
     gene            complement(5427..6302)
                     /locus_tag="ACG8KR_002386"
     CDS             complement(5427..6302)
                     /locus_tag="ACG8KR_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0201709.1"
                     /translation="MTTNKRWSELVQNTVKKVMPDGYHFNYIQCSQTTESIYFSILNE
                     NRLYYFRVSSHHRKKREDNVTTFLSGKYNGFKELSEAIGEYLKSNRFVRISDKHILIL
                     LLYLNNSFKLFWRDDTFYYIENGMDIYSGINGILKHYIKALLRYGVLYQEDLSNQIKI
                     SNSGKSLLAKYKKLLSPIYKGIDMEAVNSETLFNLVVDEKFANEFFKSKKIERLNNLF
                     PENAIKAYVDNNKSRLYNWFIPKRLRGKIKIGDQVKVINLNHTKIVTVTELYIAEKED
                     ITKMKPVIKILDNKE"
     gene            complement(6519..6776)
                     /locus_tag="ACG8KR_002387"
     CDS             complement(6519..6776)
                     /locus_tag="ACG8KR_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0201710.1"
                     /translation="MINEDFRSENKMIDISLIEKLLFSNKYTAKELATRWQISESAIK
                     AYRQNKGSVGWRDWKGISIGKAEDIMKREGFQADNDSKKVE"
     gene            complement(6779..7003)
                     /locus_tag="ACG8KR_002388"
     CDS             complement(6779..7003)
                     /locus_tag="ACG8KR_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0201711.1"
                     /translation="MNQRRRKRLEEISERLSELKDELEVIQEEEYKENIPKNLQGSER
                     YEKAEESVDQLEEAVDGLSDILELIEDIAS"
     gene            complement(7176..8219)
                     /locus_tag="ACG8KR_002389"
     CDS             complement(7176..8219)
                     /locus_tag="ACG8KR_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HFQ0201712.1"
                     /translation="MKKFLVGILFTMGLVLLSPSVSAESSISMYRMYNPNSGEHLYTR
                     SVGERDNLKRYGWHYEGVAWQAPTSGTPVYRLYNRYNGEHFYTVDAGERDKISHTGWT
                     YEGVAFYSYNGANRVPVTRLYNKYVNWHHYTLDQNEKNVISKQGWKIEGTGWYAMPNS
                     VPNPTPAPSTEKVLNVPVVWQGNTMLCEGASLLEGLHYKGVATNQSLTSFVNYMPKAN
                     DNNPYHGYSGEWRHNVNGTYQGMMADPVVKWAKNVGGNAVNITGSGTNGIKNEIRNGN
                     PVVTWITYNYATPEFKQMPWGRAVWNGHVVLVDGFRNGAYHVVDPVFGAKWINASTYE
                     RAFNVTGMAVAVR"
BASE COUNT         2339 a         1703 c         1041 g         3240 t
ORIGIN      
        1 catgaaaaat cctccagtgt gttttttatt attctacaca ccttattttt tctgtctagt
       61 ctagtgtagc cgattcagct tatcgctact tgctgattca tcatatttag ttcctagatt
      121 atctaaactt agcatataag aaattttttc ctgatctgtt agttttgaaa atgaactttc
      181 ttttaaactt tggattatac aatcagcact aaatatttcg tttaataaag cagttttctt
      241 ttctaattcc ttagaagata aagttagttc tctcatagtg acttacctac tttccaaatt
      301 ttgtgtccta tttatcactt ctctctttat ataagtgata aaagatcgta aatacgttta
      361 attgttaagt tcatattcac atgattatcc aaataaaata tcttatcttt attagaaatt
      421 tcactcataa cacgataata tagaccatga ttcaagtaat ctgtatatag aaaaatcgca
      481 tcacacgtat tcaaattttt aagtatcttt ccattatttt cgcttacatc aaacatttta
      541 atatttggta atagttcagt taatttattt tgaaaattta catgtcctcc cactaaaaga
      601 atgtttttat gttttagata agctatcatt tcagacagtg aaggctcttc agttttacca
      661 tttaatgatt gtctatgttc ttgctcctgc aattgcttta atactttatt ttcaaacctc
      721 agctgttcaa gttcttttct gattcctttt aattctttaa tctcttgctt caaaagttcc
      781 tcttcttttt ttgccgattt gactaaagat tctaaccgac cgttttcttt actcgcaata
      841 cttaattctt ttcttaattt gattacttca gatttgtttt cttttctaat acttgcaatt
      901 tgttttgcta tttcataatc agaattcagg atcaattctt ccttagcatt tttaaacaac
      961 cgagttaaaa cgaactgata taacaaacta taaaaaacta gcaaatagtc aatttgtcgg
     1021 tactcggtat tctgatcaat tgcatataga gagattaaaa cgaaatacac aatatcattc
     1081 agctcgctac ccttgatctc aaaattattg actaagtaac tatcaatacc catcaaatct
     1141 gcaaaatctc catacaaaga aaaatatgat tttgacaata tatctatatc tgaatcgtca
     1201 aacccattta ctattttccc atcattgtta gtagttgcta acgaagcaat agtgactttt
     1261 gaattgggtt ttacaaatcc tatacctaat agttctttca tctttttaat ttcaagtttg
     1321 ttatcctttt gttcggcttt atgctgtttc atattgtaaa gcggataaaa tgctgttccg
     1381 taagcacctt ttagtacgtt atctataaaa taatcttcag aacctgtttt gaatagaaag
     1441 ttaatcgcag ccaaattaga taacagctct tcagtgttgt gcatcaattt tttatcattt
     1501 aataaattat ccccaagata tttcattgca taataagggt ttgtttcgct acttattttt
     1561 aatatttttt gatagctatt cctcacgata ttaaccaaaa tatctgtctg attaattgaa
     1621 ttacagtaca aagccactcc taatgccata taaacattca aatcatgttt tgccgtaccg
     1681 ttgaataaaa cgtcttcaac ttttgaatta tctcgtctat actttttgat catatttttg
     1741 atattttgat cattctcttg gtatattttt ttcactcgtt ccaatgcctt gggtttgttg
     1801 gaaacagcaa cagcgaagtc tagttctttg aaatcaatat ttagtatctg tacctcatca
     1861 cctagattat aatctctatt tacgctcgga aaactgctga aatctaactg ttttttacct
     1921 atttttggcg aaaatattcc ttcgatttct ggcaaatgta atcacactct ctccttattt
     1981 atctttagaa aatttgtcac tattctcctt atatttatca ataaaccttt ttaatcgagc
     2041 aatataagcg ttaacccttt taggttcttc ttttcgccat tcatcgttta cgtgaccatt
     2101 ttcaaagaaa gaagctaaaa gagtttccac ttcattaacc atatctgcga tcgtatattt
     2161 ttctgtaccc tcactcaatt attcatgaac tgttaagtgt tcatagctaa gttcattcat
     2221 aattttgtta tagcgttttc ttgctttttc aaattctttc tgttcgtcaa attccacttg
     2281 attcttcatg ttgttcttcc tctctgtttg atattttttc acttcttcaa gtttagcatc
     2341 tagttccgct ttaacttctg tcacttcttt aagcgcttgg gataacttat gagacaattt
     2401 cgcataagtt tctctcttgt attcatttcc tactcccaac tcttcggagt agaactcttc
     2461 tagtagtctt gcaaaattaa catttctcct gccagcatag ttttttagct tctcaaaatc
     2521 agattgaaag acagcaacac ttttgtaatc cttttcaccc tcaaaatcaa aagcatagtc
     2581 atactttcct gaaatcaatt catcaataac tgtagcaata tattgagaga tagaaatatt
     2641 atcttcactt ctatgtcgct ctataagatt atatatatca gggctgacat gaaacgtact
     2701 tctctttttt gtttgccttt ttctaactgt tacgaccact tttggaactt cactatctcc
     2761 attgatctct ttgtattttt caataaataa ttttctagct tgttctcgac tttttgccgg
     2821 aatttcatct acaattttat cttctaaata aatatagtgt gttctcatac attcgtcctc
     2881 tatcttcttt actctcaatc ttcatgatct tagttcttac cttgtttact cttaaacatt
     2941 gcaaaaaaat ctagtcaaaa tcaaacttga aatcatcgtc tatcacaaca cccttaactt
     3001 catctttaga tttcccaaag gaacccttat tttcaaatcc tttatttttg aatgatgatg
     3061 gttatggaaa acttcctttg aaattctgac aagcataact agtcagaact gttttactta
     3121 gattaagctt tcaaaagata ctgatgaaga actaagttct cagtaccttc ccattcttca
     3181 ataaaaaata tttctccttc ttttacccac tctatagcta atttcttata atcaaataaa
     3241 ccatatgtgt ttaagccttt gtctttcatt aaactcaaaa actgatcccc ttttaatctt
     3301 tctgtatcat ccatcagaaa ttcaactaaa tctttatcca tgcgtgcaac tttttcgtct
     3361 tttagtgcca ttgatttagc atagtcagtt gaatctttta gaataataac acctacattt
     3421 ccttccacaa ttttcttctt catatttacc tcctaccatc ttttccaaag agtatttagt
     3481 caaaatcaaa ctcgaaatca tcgtctatca caacaccctt aacttcatct ttagatttcc
     3541 caaaggagcc cttattttca aatcctttgt ttttaaattc taaagctttt tcgacttctt
     3601 ctttggatat tgtatgaact tttttcttaa ctactccaag tttatacagc tcttcaaact
     3661 ctggtacttc ggatctgaaa tacaatccat cagtacgaat ttttaatagc ccatatgcgc
     3721 tatgaacgta tttctgttca tatctaacca tacgatctct aaatacgcct gctaccatgg
     3781 aggaatacgc tcgattatac atactgtatt tatcgaaggt gcttgtttct ccttctctta
     3841 atgatttttg tccgatagca ccaaatactt tcgttctaag atttttataa agcttcaacg
     3901 tatcaggatc agtttttaat ggctcgattc tttcaaaata ttctttgagt aatccttttt
     3961 tcgtcaaaaa tgtgtggcta tctaggactt ccatctcttt aatatcataa taatcaagga
     4021 taagtgttag ctcaatctcc gataaccaaa taggctcatt agctgttttg ggattttcgc
     4081 cataatccgc aaacatcgac tgtgattttg gattagcata tgttccttct ttggctgttc
     4141 cttcaaaatt aaccaaaaag aacatatacg tttttaaatt ttcatacggt cttacttttg
     4201 gtctatttat cgctctcaaa atagcttgat ctgtattata ttttgctttc aaataagtgt
     4261 ttgacggcaa aggataattt ctcatgatcc tcgaataggc ttcgttgaaa tcatattcga
     4321 tcatctttgc tccttcctca aacaaatcca agttatacat gctaccggag gtactggctc
     4381 tataggctga acttggaaca aagaaaggtg catcttccgg cttatcatag cccaaaaaat
     4441 gtattcctgt ttgcttgttc tgacggtaaa tgtccatcaa aagatatttg atcgtactaa
     4501 aagaatctaa ttttcctctt gtcatccacc ctaatccgac aagtctttta attttattct
     4561 gcatttcagg tgtgtatatg ctattgtttc ccatttatct gctcatctcc ggctttttta
     4621 ttctagaccc tatatttgtt cattacacca cttatctcgt ctacaaaggc tcccagatcg
     4681 tgtgtaaagg catcatagta atcttcaatc tcactcgatt cagttctccg gtttcttccg
     4741 tttttatttc tgaatagata catctgcgtt tctcttaaat cccttgcttt tttcgcaagt
     4801 gctaataaat ccgtaaaatt atcaataacc ataatgtcat tgatcataaa accttcttca
     4861 tgaccatact tttcagccat ctcgataaaa tcattaacat cgaacaactc caataattct
     4921 gctttatatt tctctaacaa tgtatttaaa tagcgatttc tcaattccca tgaactttca
     4981 ccagttccgt attcaacctt gatccctaaa ttcttagtgt agccttttgc tgtgtatcgt
     5041 ccaatgaagt ctcccttatt ccgattgcta tcttcttctt tgaaaaactc acgccaaatc
     5101 ccctttttct tcttaggaat tagacctctg ctcttgaaat catcatattt ttttgaagct
     5161 tctcttcgtg tatctaagta cctttgcttc agctctgtag ctgattcatt cgttccatat
     5221 tcaacagtga tccttaagcg cctgctataa ccttttgctg tatatcgtcc aacaaagtca
     5281 ccgccacttc gatcactact gacttcctta acaaattctc tccatatacc ttttttgtcc
     5341 tttggtatta atcctcgtct tttcatatct tgatattttt cagaagcttc ttttagagta
     5401 ttcttttttg ctaccatata ttccactcac tctttgttat ctagtatttt aataactggt
     5461 ttcatttttg taatatcttc tttttcagct atatacaact ccgttacggt aacgatctta
     5521 gtatgattta gattaattac tttcacttga tcgccaattt tgatttttcc tcgcaaccgt
     5581 ttgggaataa accagttgta taatcgtgat ttattattat ctacataagc ttttattgca
     5641 ttttcaggaa acaggttatt tagtctttca atttttttac ttttaaaaaa ttcattcgca
     5701 aacttttcat caacaactaa attaaataaa gtttcagaat ttacagcttc catatctatt
     5761 cctttgtaga taggagaaag caatttttta tattttgcca gtaatgactt tccagaatta
     5821 ctaatcttaa tttggttact taaatcttct tgataaagaa ccccatatcg aagtaacgcc
     5881 ttaatataat gttttaatat gccgttgata ccagaataaa tatccatgcc gttctcaata
     5941 tagtagaaag tgtcgtctct ccaaaacaat ttgaagctat tattcaaata aagtaacaag
     6001 atcaatatat gtttgtcaga aattctgaca aatctattac tttttagata ctcaccaatg
     6061 gcttcactta actctttgaa gccattgtat tttcccgata aaaatgtcgt aacattatct
     6121 tctctctttt tcctatggtg tgaacttacc ctgaaataat agagtctatt ctcatttaat
     6181 attgaaaaat aaatactttc agttgtttgt gagcattgta tatagttgaa atgatatcca
     6241 tctggcatga cttttttaac tgtattttgt actagctctg accacctttt gttagtagtc
     6301 atatattttt ttccattaaa ccaaacataa atatcttttt taaaaatttt gttccccccc
     6361 tattgcgttt tctttgacat taaagccctt tgttttcatc acattcgtat agccctagcc
     6421 actcataacc tgatgctttt gcgaagttta tttaaaagtg ttttcctttg taatctacct
     6481 ttcccctcgt cctggtactt ttcttggcga ctattaaact attcaacttt tttagaatca
     6541 ttgtcagctt gaaatccttc acgtttcatt atgtcttctg cttttccaat gctgattcct
     6601 ttccaatcac gccatcctac gcttccctta ttttgacgat aggctttaat agctgattcg
     6661 gatatttgcc atcttgtagc caactctttg gctgtgtact tatttgaaaa aagtagcttc
     6721 tctatcagtg aaatatcaat cattttattc tcactcctga aatcttcatt aatcatctct
     6781 agctagctat atcttcaatc aattctaata tgtcggataa accatcaaca gcttcttcaa
     6841 gttgatcaac ggattcttct gctttttcat atctttctga tccctgtaga tttttaggta
     6901 tattttcttt gtactcttct tcttgaatta cttctaactc atcctttagc tcggaaagtc
     6961 gctcgcttat ttcttctaac ctttttcttc ttctttggtt catcaaaatc acctcttcgc
     7021 ataatctcta cgattttaat ataccacttt tgtgtactac attcaatatt ttttgttata
     7081 gattcgtatt acaaaaattt cctctaaaaa aataagagcc tatttaccat ttcagtaatt
     7141 aagctcttat ttcatttagt tgataattct atcaattaac gtacagcaac cgccattcca
     7201 gtcacattaa atgctctctc atacgtgctt gcattgatcc attttgcgcc aaatacaggg
     7261 tcaacaacat ggtatgcacc atttctgaag ccatccacga gaactacatg tccattccat
     7321 actgcacgac cccaaggcat ttgtttgaac tctggcgtag catagttata agtaatccaa
     7381 gttacaaccg gattaccatt gcgaatttca ttcttgattc cattcgttcc tgaaccagtg
     7441 atattgacgg cattaccgcc aacgtttttt gcccatttaa ctactgggtc agccatcatg
     7501 ccttgatacg ttccattaac gttatgacgc cactctcctg agtaaccatg ataaggatta
     7561 ttgtcattag cttttggcat atagtttaca aaactggtta agctttgatt tgtggctaca
     7621 cctttgtagt gtaaaccttc taataaagaa gctccttcgc ataacattgt atttccttgc
     7681 catacaacag gaacgttcaa cactttttca gtgcttggag caggtgtagg atttggtacg
     7741 ctgtttggca ttgcatacca gcctgtacct tcaatcttcc aaccttgttt actaattacg
     7801 ttcttttcat tttgatctaa cgtgtagtga tgccaattca cgtatttgtt atataaacgt
     7861 gttacaggaa ctctatttgc tccgttgtag gaatagaacg caacaccttc gtacgtccat
     7921 cctgtgtgtg aaatcttatc tctttctccg gcatcaactg tgtaaaaatg ttcaccgtta
     7981 taccgattat acaaacgata aacaggagta ccacttgttg gagcttgcca tgcgacacct
     8041 tcgtaatgcc atccataacg tttcagattg tctctttctc caacgcttct agtgtataga
     8101 tgctctccgc tattgggatt atacatacga tacatggaaa tagagctttc tgctgaaact
     8161 gaaggtgata ataatactag ccccattgtg aatagaattc ctactaaaaa ttttttcatt
     8221 tgattttacc ccttttcatt ttttattgtc attattgcat gaaaattatt cgtaagattg
     8281 ccaattacat aaacgattgt tttaagatca taatcgttgt tgt
//
LOCUS       DAZKLD010000127         8268 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.127, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000127 DAZKLD010000000
VERSION     DAZKLD010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8268)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8268)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8268
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.127"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            157..2256
                     /locus_tag="ACG8KR_002390"
     CDS             157..2256
                     /locus_tag="ACG8KR_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division site-positioning protein MapZ
                     family protein"
                     /protein_id="HFQ0201713.1"
                     /translation="MKKCPNCGSKKMEQNTICPDCGHQITAEDQQIKESIEETNTDAA
                     MQDKSTEDAVFPDTELNDPIEWSELKDLPLESVMELFEKAEPEDEEPPAHDQVTNIEK
                     NEQKKATVHETSEESGKNQEETLESKQKRRVAELKEIVDNEEENSILSAYIKAHREDT
                     KEEHAKELLKMISEKIAQENKVEPEKEQETEKDSDRENATGKETVKIKDVKTDITEKT
                     EVFTEKQLEKEKTNKLPEAEKKEILENTDKKSTEPDEINSESDAENQSVSKEGTDLEA
                     KIEKEENQPKLFEAEAKSITIVPDTEKTHQESNLEKTDPDKAQKKKSKKVPYLVLAAV
                     LLLGGGSWAYYDHHQKVEAEVAAEAKRQQQMEELHEKLAAFYTDDHHQFIRADMIHQD
                     LSKLKNDLSSLKNEKGYSELETTYQDIQSKIRDIQKVNEWFIAPAIVDDHLADDIKLK
                     ADKEIQAIETDNTDFGKLIQQAQTEAKNQYAELQTAKEKTAIVFKDGKVVDSATRDQY
                     KTAKEAVEKVKNTDLSAQLNEQLKQVDKMLTDKEKKEEEEKKKAEEAKKAAEEQAKQA
                     AQAAAAAQAAQQAAVAQAAQQEAASSTPSSSNSANQPIKSARASDIADASNPAWTWAP
                     GVQSKVIATCIQRGYIVEGGYRLEKARIENGEGYYNLYATSTKSALMKGIGESALPFY
                     IVTINCKTGWFGGNGSN"
     gene            2452..3315
                     /locus_tag="ACG8KR_002391"
     CDS             2452..3315
                     /locus_tag="ACG8KR_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor B family protein"
                     /protein_id="HFQ0201714.1"
                     /translation="MNDLLATIFTGIIIDENEKNYFVQKNGQTFGLSKSEGEHKIGEA
                     VEGFGYQNQKHENRITTQIPKSRIGHYAFGIVTATRKDLGAFVDIGLPDKDFVVSLDE
                     LPTMTELWPKKGDRLMISLRVDAKDRIWGSLADEKIFKSLSKYGTEEMKNKDVIGTAY
                     RLKLTGTYVLTEDFYLGFIHPSERYNEPRLGEQIKGRVIGVRPDGVLNLSLKPRAYEA
                     ISDDAQMILTILERSADQQINFTDKSDPEEIMKAFGISKGAFKRALGNLLKQGLITQE
                     NGVTKLVKKSN"
     gene            3420..3893
                     /locus_tag="ACG8KR_002392"
     CDS             3420..3893
                     /locus_tag="ACG8KR_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HFQ0201715.1"
                     /translation="MVMNAISAMKKTKQQLHEAGFKLTPQREATLLVLLENEKEHLSA
                     EEIFFLVKKKSPEIGLATVYRTLEILTELKIVDKVSFNDGLARYDLRKEGAKHFHHHL
                     LCLNCGTIEEVEEDLLLEVEQVVEERYHFLVKDHRLTFHGICQECYEKEKRNQSV"
     gene            3989..4876
                     /gene="xerD"
                     /locus_tag="ACG8KR_002393"
     CDS             3989..4876
                     /gene="xerD"
                     /locus_tag="ACG8KR_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HFQ0201716.1"
                     /translation="MKEQINDYLHYLNIERGLSANTRQSYERDLEQYLHFLQEQKIAS
                     WQDVDRFLVIRFLEKLHEEKKATATVTRMISSLRRFHQFLRQERITDHDPMQHIDTPK
                     KAQKLPSTLTLTEVEKLIEAPDTTKTLGIRDRAILEVMYATGMRVSELIGLKLGDLHL
                     SLGLVQTVGKGDKERIIPLGDYAIQWLERYLDEARPLLVKDASEMHVFVNNHGKGLSR
                     QGIWKNLKQLVREAGIYKEVTPHTLRHSFATHLLENGADLRTVQELLGHADISTTQIY
                     THITKKRMTDVYKQHFPRA"
     gene            4895..5266
                     /locus_tag="ACG8KR_002394"
     CDS             4895..5266
                     /locus_tag="ACG8KR_002394"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0201717.1"
                     /translation="MLTHYRKENRKIAMGLLSFHKKLTGKTSLLKEIDTYESEDDYEL
                     FFFTPEGSTNIQGIIGIYWATNDQLIIHDISLNPSYRGENMGFQMLDELQEKYPDVSI
                     RATEITEPYIGKWLAKKKKKE"
     gene            5263..6033
                     /locus_tag="ACG8KR_002395"
     CDS             5263..6033
                     /locus_tag="ACG8KR_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HFQ0201718.1"
                     /translation="MNELTEINIKLDVFEGPLDLLLHLIQQMEIDIYDIPIATITEQY
                     MNYIHTMKTLELAVAGEYLVMAATLMAIKSKTLLPVQEFVTDDEELWEEDPREELVTQ
                     LLEYRKYKYAAERLTEKAEERSLYYTKEPMNIDGLADAEKPLKRGQVKKIDLIRAMQK
                     ILERKKFSQKVEKTITPDDTTIEERMVFIEERIKRNAHGCTFDSFFESPSKSEVVTTF
                     MALLELMKKGQIIAQQKENYESIMLYPAVGDTEHDEIK"
     gene            6017..6586
                     /gene="scpB"
                     /locus_tag="ACG8KR_002396"
     CDS             6017..6586
                     /gene="scpB"
                     /locus_tag="ACG8KR_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HFQ0201719.1"
                     /translation="MTKLSELEALLFIVGEEGISNEELSGLLNLSEAEVTSLVKELKE
                     SYTQNSHSALHIFETESHYVLTTKKELAPLLKRYAQGTSNTLSQAAVETLAIIAYKQP
                     ITRAEIEQIRGVQVSGAVQRLAAHKLIEEKGRVEGPGRPILYGTTGYFLDYFGLKSLE
                     ELPDTQEMEISLEEDVSPDLFFDGMNKGE"
     gene            6592..7308
                     /locus_tag="ACG8KR_002397"
     CDS             6592..7308
                     /locus_tag="ACG8KR_002397"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HFQ0201720.1"
                     /translation="MERLQKVIAHAGITSRRKAEEFILAGRVKVNGQVVRELGIKVGK
                     HDTVEVDEVPIYQEEYGYYLLYKPKGVISAVADDKGRKVVTDLLPMVKERIYPVGRLD
                     YDTSGILLLTNDGDFAQRLTHPKHEVDKVYVAKVKGIATKSMLAPLTKGIKIEGRKTS
                     PAKFQILSVDPKTNHSIVELTIHEGRNHQVKNMLQAVGLPVQKLKREKYGDLTLQGLR
                     PGDYRELNKKEVSSLLNHSK"
     regulatory      7390..7515
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            7638..8249
                     /locus_tag="ACG8KR_002398"
     CDS             7638..8249
                     /locus_tag="ACG8KR_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFQ0201721.1"
                     /translation="MKNTRVQKMVAVAMFAAIALVLQYFAFPIMPAFSFLKIDFSDIS
                     VLISMFLFGPAAGVATAFLRSVLHLLTTGFSPDNLVGDTASFLSTTIFTFPIYYFFSV
                     KKQHKGRNKFLGVITGTIALTIFMSIANYFVITPLYLMFLGLNATQMLGMSLANYVLI
                     GIVPFNLIKGFIVSAVFLVLHAQLLPWLSRKQHQFEQRHTAVK"
BASE COUNT         3014 a         1418 c         1747 g         2089 t
ORIGIN      
        1 gcttgagact acgcagtctc aagctttttt ttctatagga aggaaattca ttcattattt
       61 ttaagtaaat cctaaaaata agctattggc gtgacgtata gaatatgata gattaaatgg
      121 tagcgtaaac ctagtaaagg agccgatcgc taagtgatga aaaaatgtcc aaattgcgga
      181 tcgaaaaaaa tggagcagaa tactatctgt ccagactgcg gtcatcaaat aactgcagaa
      241 gatcagcaaa taaaagaatc aattgaagaa acaaatacag atgcagctat gcaggataaa
      301 tcaactgaag atgcagtctt tcctgataca gaattaaacg atccaatcga atggtcggaa
      361 ttgaaagatt tacctctaga atctgtaatg gaattattcg aaaaagcaga accagaagac
      421 gaagaaccac ctgctcacga tcaagtaact aatatagaga aaaatgaaca gaagaaagca
      481 actgttcatg agacttcgga agaatcagga aagaatcaag aagaaacgct tgaatcaaaa
      541 caaaaaagaa gagtagctga actgaaagaa atcgtcgata atgaggaaga aaattcgata
      601 ctatccgctt atatcaaagc acatcgtgaa gatacaaaag aagagcacgc gaaagagctg
      661 ctcaaaatga tcagtgaaaa aattgcacag gaaaacaaag tagagccaga aaaggaacaa
      721 gaaactgaaa aagactctga tagagaaaac gccacaggta aagaaacagt aaaaataaaa
      781 gacgtaaaaa cggatatcac agaaaaaact gaagtattta ctgaaaaaca attagaaaag
      841 gaaaaaacga ataaactgcc ggaagcagag aaaaaagaaa tactcgaaaa cacagacaaa
      901 aagtcaacag agcccgatga aataaatagt gaatccgatg ctgaaaatca atctgtaagc
      961 aaagagggta ctgatcttga agcaaaaata gaaaaagaag agaaccagcc taagctgttt
     1021 gaagcagaag cgaaatcaat aacaatcgtg ccagatactg aaaaaacaca tcaagaatca
     1081 aacttagaaa aaacagatcc agataaagcg caaaagaaaa aatcaaaaaa agtaccttat
     1141 ctcgttttag cagcagtact tctactcggt ggaggaagct gggcttatta tgaccatcat
     1201 caaaaagtag aagccgaagt agcagcagaa gcaaaaagac agcaacaaat ggaagaactt
     1261 catgagaaac ttgctgcctt ttatacagat gatcaccatc aatttattcg tgcagatatg
     1321 attcatcaag atttatcaaa actaaaaaat gatttatcca gtcttaagaa tgaaaaagga
     1381 tattcagagt tagaaacaac ttaccaagat atccaatcta agatccgaga tatccaaaaa
     1441 gtgaatgagt ggttcatagc acctgccatc gtcgatgatc atctagcaga tgacatcaag
     1501 ttgaaagcag ataaagagat ccaagcaatc gaaacggata acacagattt tggaaaattg
     1561 attcaacaag cacaaacaga agctaaaaat caatatgctg aattgcaaac agctaaagaa
     1621 aaaacagcta tagtctttaa agacggaaaa gtagtggatt cagcaacacg cgaccaatac
     1681 aagacggcaa aagaagcagt agaaaaagtg aaaaatacag atctatctgc tcagttgaat
     1741 gaacaattga aacaagtaga taagatgctc actgataaag agaaaaaaga agaagaggaa
     1801 aagaaaaaag cagaagaagc gaaaaaagca gctgaagaac aagcaaaaca agccgctcaa
     1861 gcagcagctg cagctcaagc cgctcaacaa gctgcggtcg cacaagcagc acagcaagaa
     1921 gcggcatcct cgaccccttc ctcttcaaac tcagcgaatc agccaatcaa atctgccaga
     1981 gcaagtgata ttgccgatgc atctaatcca gcatggacgt gggcaccagg tgtccaaagc
     2041 aaagtgattg ctacatgtat ccaacgtgga tatatcgttg aaggaggtta cagattagaa
     2101 aaagccagaa tcgaaaatgg ggaaggctat tataatctat atgccacttc aacaaaatct
     2161 gcattgatga aaggcattgg agaaagtgct ttgccatttt acatcgtaac catcaactgt
     2221 aaaactggat ggttcggagg aaatgggagc aattaatttt aggcaaatga aagaggctgt
     2281 gagcctgccg tttaacaccg tttattcgaa aaaggggagt atgacacaac tgttgtcata
     2341 ctcctttttt cacctttcca aaccaaggct cagctattca agtagataac attatgttat
     2401 gatagcaaag aaatgtatta agaaaacctt tcactataga gggagaaaaa aatgaacgat
     2461 ttattagcaa ctatatttac aggtattatt attgatgaaa atgaaaaaaa ctactttgtc
     2521 caaaaaaacg gacaaacctt tggcttaagc aaatcagaag gtgaacacaa aatcggtgaa
     2581 gcagttgaag gattcggtta ccaaaatcaa aaacatgaaa atcggatcac tacacaaatt
     2641 cctaaaagcc gtatcggtca ttacgctttt ggaatcgtta ctgctacacg aaaagattta
     2701 ggtgcgtttg tcgatatcgg tcttccagat aaagactttg tcgtatctct tgatgaattg
     2761 ccgacaatga cagagctatg gcctaaaaaa ggggatcgct tgatgatttc ccttagagtt
     2821 gatgcaaaag accgtatttg gggcagttta gcagatgaaa aaatattcaa atctttaagc
     2881 aaatatggaa cagaagaaat gaaaaacaaa gatgtgatag gaacagcata ccgcttgaaa
     2941 ttaacaggta cgtacgtttt aacggaagat ttctatcttg ggtttatcca tccttctgaa
     3001 cgatataatg aacctcgtct aggcgagcag ataaaaggtc gagtgattgg tgtccgacca
     3061 gacggtgtac tgaatctttc attgaagcca agagcatatg aagctatatc agatgatgca
     3121 caaatgattt taaccatttt agaacgttct gcagatcagc aaatcaactt tacagataaa
     3181 tcggatccag aagaaatcat gaaagctttt ggtatcagca aaggagcatt taagcgcgca
     3241 ttaggtaatc ttttgaaaca aggattgatt acacaagaaa acggtgtgac aaaactagtc
     3301 aaaaaatcta attgagaatc ccttgcatgt ttttcttaac tagcttataa taattttaat
     3361 taacatagtg ttatatcttt ttttatttgt aattattata aattgttggg gttgagtaaa
     3421 tggtcatgaa tgctatttcg gcgatgaaaa aaacaaaaca gcagctacat gaagcggggt
     3481 ttaagctgac accgcaaaga gaagctactc tgcttgtgct tttggaaaat gagaaagagc
     3541 atttatctgc agaagaaatc ttctttttag tgaaaaaaaa gagtccggaa attggacttg
     3601 ctactgttta tcgaaccctt gaaatcttga cagaattgaa aattgtcgat aaagtaagtt
     3661 ttaatgatgg attagcccgt tatgatttga gaaaagaagg cgccaaacat ttccatcatc
     3721 atttactatg cttgaactgc ggcacgatcg aggaagtaga agaggatctt ttacttgaag
     3781 tggagcaagt agtagaagaa cgttatcatt ttttagtaaa agatcatcgg ctaacgttcc
     3841 atggaatctg tcaggaatgc tatgaaaaag aaaaaagaaa ccagtccgtc tgagtgtcta
     3901 gacggctttt tctttgctca gattatggaa aaagactatc agaatgaagc ggtatctgat
     3961 atgatagaga ggcatgaggg ggattattat gaaagagcaa atcaacgatt atctccacta
     4021 cttgaatatt gaacggggat tgtccgccaa tacacgacaa agctatgaac gtgatttgga
     4081 acagtacttg cattttctac aggagcagaa aattgcttca tggcaggatg tcgatcgctt
     4141 tttagtcatc cgttttctgg agaagttgca tgaagaaaaa aaagcgacag caactgttac
     4201 tcggatgatc tcaagtctca gaagattcca tcagttttta cggcaagagc gtatcactga
     4261 tcatgatccg atgcaacata tcgatacgcc gaagaaagca cagaaattac caagtacatt
     4321 gactctgaca gaagtagaaa agctgattga agcacctgat acgacaaaaa cgttagggat
     4381 aagagatcgt gcaatcttgg aagtcatgta tgctactggg atgcgcgtca gtgaattgat
     4441 tggattgaaa ttgggagatc ttcatctttc tcttggactg gtacagacag ttggaaaagg
     4501 agataaagag cggatcattc ctttaggtga ttatgcgatc caatggctgg aacgctactt
     4561 agacgaagca cgtccattac ttgtaaagga cgcatctgaa atgcatgtat ttgtcaataa
     4621 tcatgggaaa ggtctttctc ggcaaggaat ctggaaaaat ctgaagcagt tagtacgaga
     4681 ggctgggatt tacaaagaag taacaccaca tacgctgcgg catagctttg ccacacatct
     4741 gctagaaaat ggagctgatt tgcggacagt acaggaattg ttaggtcacg cggatatctc
     4801 tacgactcag atttatacac atatcacgaa gaaaagaatg acggatgttt ataagcagca
     4861 ttttccaagg gcttagtcat aggaaggtga gaagatgtta acacattacc ggaaagaaaa
     4921 ccgtaaaatt gctatgggac tattgagttt ccataagaaa ctgacaggta aaacttcttt
     4981 gttgaaggag attgatacgt acgaatctga agacgattat gaactttttt tctttacgcc
     5041 agaaggttca accaacattc aagggattat tggtatttac tgggcaacga acgatcagct
     5101 gatcatccat gacatttctt tgaatccgtc ttatcgcggt gagaatatgg gctttcaaat
     5161 gttagatgaa ttacaggaaa agtacccgga tgtctccatc cgtgcaactg aaatcacaga
     5221 gccttacatt ggaaaatggc tagcaaagaa aaagaagaaa gagtgaatga gttgacggag
     5281 atcaatatta aattagatgt attcgagggt cctctcgatc tgctgcttca tctgattcag
     5341 caaatggaaa tcgatatata tgatataccg atcgctacga tcacggagca atatatgaat
     5401 tatatccata caatgaaaac actcgagcta gctgttgcag gagaatactt agtgatggct
     5461 gctacattga tggcaatcaa aagcaagaca ttgcttcctg tccaagaatt cgtcacagac
     5521 gatgaagaat tatgggaaga agatcctcga gaagaattgg tcactcagct tttagaatac
     5581 cgtaaatata aatatgcagc tgaaagactg acagaaaaag cagaggaacg aagtctttat
     5641 tacacaaaag aacctatgaa catcgatggg ttagcggatg ctgaaaagcc gttgaagcga
     5701 ggacaagtca aaaaaatcga tttgatccgt gccatgcaga aaatcttaga acgtaagaaa
     5761 ttttctcaaa aagtggaaaa aacgatcacg cccgatgaca caacgatcga agaacgaatg
     5821 gtatttatcg aagagcgtat aaaaagaaat gcgcatggct gtactttcga ttcttttttt
     5881 gagtcacctt ctaaaagtga agttgtcact acttttatgg ctttgctaga attgatgaaa
     5941 aaagggcaga tcattgcgca acaaaaagaa aactatgaat caattatgct ttatccagca
     6001 gtaggagata ccgaacatga cgaaattaag tgaacttgaa gcccttcttt ttattgttgg
     6061 cgaggaaggg atcagtaatg aagaattaag cggactgtta aatctcagcg aagcagaagt
     6121 cacaagttta gtgaaagaat taaaagaatc ttacactcaa aactcgcact cagctttgca
     6181 tatttttgaa acggaaagcc attatgtatt aacgactaaa aaagagctgg ctcccttact
     6241 taagcgatat gcgcaaggga catcaaacac attatctcaa gccgccgtcg aaaccttagc
     6301 aatcatcgct tataaacagc caatcacaag agctgaaatc gagcagattc gaggagttca
     6361 ggtttcagga gcggtacagc gtctagctgc ccataaactg attgaagaaa aaggtagagt
     6421 agaaggacca ggtcggccga tattatatgg aacaactggc tacttcttag attattttgg
     6481 tttgaaatca ttagaagaac tgcctgatac acaagaaatg gaaatttcac ttgaagaaga
     6541 cgtgtcacca gatctattct ttgatggaat gaataaagga gaataattgt aatggagaga
     6601 ttgcagaaag taattgccca cgctggcatc acttctcgtc gaaaagcaga agaattcatc
     6661 cttgcaggac gtgtaaaagt aaacggacaa gttgtaaggg aattagggat aaaagttggg
     6721 aaacatgaca ctgtggaagt ggatgaagtc ccaatttatc aagaagaata cggttactat
     6781 ttattgtaca aacctaaagg agttatttct gctgttgcag atgacaaagg aagaaaagtt
     6841 gttacggatc ttttgccgat ggtaaaagaa cgtatctatc ctgtaggtcg actggattac
     6901 gatacttcgg ggatcttgct actgacaaat gatggagatt ttgctcagcg cctgacacat
     6961 ccaaaacatg aagtggataa agtttacgtt gcaaaggtaa aagggatcgc tacaaaatcg
     7021 atgcttgcac ctttgacaaa aggaataaag atcgaaggac gaaaaacatc tccagctaaa
     7081 ttccaaattt tatcagtgga tcctaagaca aaccacagta tcgtggaatt gacgatccac
     7141 gaaggacgta atcatcaagt gaaaaatatg cttcaagcag ttggtcttcc tgtgcaaaaa
     7201 ctaaaacgtg aaaaatacgg agatctgact ttacaaggcc tacgtcctgg ggattaccgt
     7261 gaactgaaca aaaaagaagt cagcagtttg ctgaaccact caaaatgaac acttactttt
     7321 tataagtgtt agatgttgcg ttttttgaat agatatgtat aatgaatatg taaacaaaaa
     7381 ttatataagg ttcgtcttca ggggcagggt gaaattcccg accggtggtt atagtccacg
     7441 acctattttg caaaaaaata gctgaatcgg tgaaattccg ataccgacag tatagtctgg
     7501 ataaagaaga tagagcttat ttgacgacgc ttgatcaaat aactctaact ctattttccc
     7561 tggaaaatag agttttttca tttggagaga aacgtcaaaa agttcgctga agaggagtcc
     7621 taacaggagg atttcaaatg aagaacaccc gtgtacagaa aatggttgca gtagcgatgt
     7681 ttgctgcgat cgcattagtt ttacagtatt ttgcttttcc gatcatgccg gcttttagtt
     7741 ttttgaagat cgattttagt gatatctcgg tattgatcag catgttctta ttcggtccag
     7801 cggccggagt tgcaacagcg ttcttgcgtt cagtattaca tctgctgact acaggttttt
     7861 ctccggataa tttggtagga gacactgcta gtttcttatc aacaacgatc tttacattcc
     7921 cgatttatta tttctttagt gtaaagaaac agcataaagg aagaaacaaa tttttaggtg
     7981 tcattacagg aaccatcgct ctaactattt ttatgagtat cgcaaattat ttcgttatta
     8041 cgcctcttta cttaatgttt ttagggctga acgcaacaca gatgctgggc atgtctttag
     8101 caaactatgt actgattggg atcgttcctt tcaacttgat caaaggattc atcgtgagtg
     8161 ctgtcttttt agtgctgcat gcgcaacttc ttccatggtt aagtcgaaaa caacatcagt
     8221 tcgaacaacg tcatacagca gtcaaataaa tatcaaaaag atcaagaa
//
LOCUS       DAZKLD010000128         8215 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.128, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000128 DAZKLD010000000
VERSION     DAZKLD010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8215
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.128"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            250..1989
                     /gene="yfcC"
                     /locus_tag="ACG8KR_002399"
     CDS             250..1989
                     /gene="yfcC"
                     /locus_tag="ACG8KR_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HFQ0201722.1"
                     /translation="MKKTNSSGKPKRDFTKLSTPHTYVIIFGVVIFAWILTFVVPAGK
                     FSTQDIEYKDANGETSTRTVLRQDSFRYAYELDKSYVFDQLEELQDHPAEREKLDVPE
                     KGLEKVIADGEKNLTQEKLDEISLTDDVLYDQYGENIYDTSKKLHKTAKIWGTDDFGG
                     FGFLNFVFEGLVSGDKYGSAVGIAALILVVGGAFGIIMRTGAIDAGIYAFISKTKGLE
                     RLALPLLFFAFSFGGATFGMAEEVIPFSMVMVPFVIALGYDSIVAVTVTYVASQVGNA
                     TSWMSPFSVAVAQGIAGIPVLSGATFRLIMWVVVTALAAGYMMIYAEKIRKKPESSLT
                     YKSDDYFRSHIKKTSDENKKFMLGHKLILLEMLVVLVWIVWGVTQKGYYIPEIASQFF
                     VMGLVAGIIAVLFKLDGMKVNDIASSFQSGAADLAGTAIVVGMAKGILLVLGGSDASV
                     PSALNTILHGIGTALTGVPAVIGAWAMYIFQSLFNLVVTSNSGQAALTMPIMAPLADL
                     VGVSRQVAVLAYQLGSGFMDAFTPVSASLIGVLGVARIEWAKWARFQIKMQGFLFVLG
                     TIFIMIAIAIGLQ"
     gene            2146..3330
                     /gene="iadA"
                     /locus_tag="ACG8KR_002400"
     CDS             2146..3330
                     /gene="iadA"
                     /locus_tag="ACG8KR_002400"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008798 - beta-aspartyl-peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HFQ0201723.1"
                     /translation="MKIIRQIEVFAPDYLGKMDVLIAGGKILAVEEQLEGGYEGVEVE
                     ELSGEGKVLTPGFIDCHFHILGGGGEGGYQNRTPEVTLSQLTTAGVTTVVGCLGTDGE
                     GRDMTALISKAKGLEAEGISTYVYEGSYRLPVKTVTDSIIKDFLTIDKIIGIGEIAVS
                     DHRSSQPSFEEFARAVADARVGGMLSGKAGIINVHLGGGKRKLELLTRIVEETEIPIT
                     QFLPTHANRTPELFEACVAFAKRGGTIDFTASEDPDFWEKTDGEVRFSKALKRLIEED
                     VSLDNFTMTSDGQGSLPYFDENNHFLGLGVGSAKALLVGIKEAVQKESIPLEIALRAI
                     TSNPARILKLDKKGKIEIGADADLCILDKETLDIDTVIAKGEIMVQEKEVKVWGTFEK
                     SF"
     gene            3441..3644
                     /locus_tag="ACG8KR_002401"
     CDS             3441..3644
                     /locus_tag="ACG8KR_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HFQ0201724.1"
                     /translation="MADLKGRSKDMKDKVVGEGKEAYGKATDDKSKETEGKAQSLGAD
                     IKEKARNLGDEVKDKFSRKDEKK"
     gene            complement(3745..4407)
                     /locus_tag="ACG8KR_002402"
     CDS             complement(3745..4407)
                     /locus_tag="ACG8KR_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0201725.1"
                     /translation="MLRTEQLGFWYQDPQDALYKDVELNFEAGKMYAILGASGSGKTT
                     FLSLISGLDAPKEGKVYYQDRSLNKIGLRSYRKNDVSIIFQAYNLLPYLSALDNVIAA
                     MEITKTKQENYRQVALDRLARVGIDEILAKKQVTKLSGGQQQRVAIVRAICCEHQLIV
                     ADEPTGNLDETTSQDIVKLFQEIAHEQNRCIIIVTHEIEVAKSCDEVFELKNKKFQKI
                     EV"
     gene            complement(4420..5922)
                     /locus_tag="ACG8KR_002403"
     CDS             complement(4420..5922)
                     /locus_tag="ACG8KR_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0201726.1"
                     /translation="MNFMKRAWKSTKVKWGRSLLLFAVFTAILIFVLAGLTIRSAAEE
                     AANQAQKEVGATVTLSANREASFKKQEQTSSSGSTTEERPDPGSFSLTPVSLTDAEKI
                     AELDNVKSYSFESSASAMAKDGITAITSSDSSEDSSETTDAGGEPAGGMGGNPQMTQA
                     DFQISGVSATAQNSNFSDGTAKITDGEGIDDSDKGTNNVVIDSTLAETNDLAVGDTFT
                     ITSTEDEDTTYEMTIKGIYESSETSGSMGMKFNFMNPSNTLYTSYTFANELNGTSDDN
                     TIDSAIYTLSDPDKMDEFLKEAEKLIDTDTFSLQSNDSMYQSMLEPLNNVASFSKNVV
                     LLVAVAGIIILTLIIMITIRERRHELGVLLSLGESRSKIILQLFTEVAICMILALGVA
                     SLSGNVVANAVGQQLLDQQTETTTQNQQAPGGGQMPGNRQEQGGQRDGQQGTTNNPFA
                     VSDQVSELEINVKPAQLGLLAGVAFGISLFSVFLASIGILRLNPRKILLN"
     gene            6470..8149
                     /locus_tag="ACG8KR_002404"
     CDS             6470..8149
                     /locus_tag="ACG8KR_002404"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HFQ0201727.1"
                     /translation="MSTIKIIPLGGVRENGKNMYVAEVGEDIFILDCGLKYPENELLG
                     IDVVIPDFTYLEENIDRVAGVFLTHGHADAIGALPYLLSKISVPVFGTELTIELAKLN
                     VSRNEAAKGFKDFHVVDEHTEIDFGETVVSFFRTTHTIPDSVGINLKTKEGSIVYTGD
                     FKFDQTAIPMYQTDYARLAEIGKEGVLALLSESANAENPQPVASEAQIADEVFDTIKY
                     WEGRIIVACVASNLQRVQQVLDAAEKAQRKVVLTGQDFERIIRTAMRLEKLQLPSEDL
                     LVTLKDMKNYDPEELIILETGRMGEPIKSLQKMANGTHRNVKIEEGDLVYITTTPSIA
                     METIVAKTEDIIYRAGGTVKLISENMRVSGHANPNDLQLMINLMKPTYFVPVQGEYRE
                     LAAHADLAHAVGMPYKNIYITGRGDVLEYKNKRMSVAGTTSAENVMIDGLGIGDIGNI
                     VLRDRKILSEDGIFVAVVTISRREKKIISKPQITSRGFVYVKASRDLMKESSNMIETI
                     VEKHLESNDFEWSKLKQDIRDQLGRYLFEQTKRRPVILPVIMEATQRRNKK"
BASE COUNT         2582 a         1447 c         1761 g         2425 t
ORIGIN      
        1 aaaaacagga cgagattttc tctaatctaa aaataaaaaa agaattttaa ggaaaaacag
       61 agaaataaaa tacttaatat aaatggaaag agaaaaaaat aatcatacat aggagaaaaa
      121 aaacaaaata gaaaaaagtg attttctaat aaattattca tgtggaaatg tcatttttac
      181 tgtataattc atttgattat ccatttttta ttcatcaaaa ttactgaaaa aagaaaggaa
      241 gaaataaatt tgaagaaaac aaattcttca ggaaaaccga aacgcgactt cactaaatta
      301 tcgacacccc atacgtatgt cattattttt ggtgtagtca tctttgcatg gatactgact
      361 tttgtcgttc cagcaggaaa gttcagcacg caagatattg aatacaaaga tgcaaatggc
      421 gaaacaagta ctcgtactgt tttacgtcaa gattccttcc gttatgctta cgagctggat
      481 aagtcgtatg tgtttgatca attagaagaa ctgcaagatc atccagcaga acgtgaaaaa
      541 ctggatgttc cggaaaaagg attggaaaaa gtcattgctg acggagaaaa aaatcttacg
      601 caggaaaaac tagatgaaat ctccttgaca gatgatgtgc tttacgatca gtacggcgaa
      661 aatatctatg atacttctaa aaaattgcat aaaacagcca aaatatgggg aacagatgat
      721 tttggcggct ttggtttctt aaattttgtt tttgaaggac tagtatctgg agataagtat
      781 ggatctgctg tagggatcgc tgcattgatc cttgttgttg gaggtgcttt tggtattatt
      841 atgcgtaccg gagcgatcga cgcaggtatc tatgcgttta tcagtaaaac taagggactg
      901 gagcgtttag ctttaccgct attattcttt gctttttcat tcggtggcgc tacattcggg
      961 atggcagaag aggtaattcc attctcaatg gtgatggttc catttgtcat tgcgttagga
     1021 tatgactcga tcgtagcagt aaccgtgact tatgtcgcat ctcaagtcgg aaatgcgacc
     1081 tcttggatga gcccgtttag cgtagcggtg gctcaaggga ttgctggaat ccccgtgttg
     1141 tctggtgcga cattccgatt gattatgtgg gtagttgtta ctgcattagc cgctggttat
     1201 atgatgatat atgcagaaaa aatccgtaaa aaaccagaaa gttcgttgac ttacaaatca
     1261 gatgattatt tccgttctca tatcaaaaag acatctgatg aaaataaaaa attcatgctt
     1321 gggcataaat tgatcttatt agagatgtta gttgtccttg tctggatcgt ttggggcgtg
     1381 actcaaaaag gctattacat tccagaaatc gcttctcaat tctttgtgat gggattagtt
     1441 gctggaatca ttgctgttct tttcaaatta gatggaatga aagtcaatga catcgcttct
     1501 tctttccaat ctggtgcagc tgatttagct ggtacagcaa tcgtagtagg tatggccaaa
     1561 gggattcttc ttgtcttggg aggatctgat gcatctgttc cttcagcttt gaatacgatt
     1621 ttgcatggga tcgggacggc gttgaccggt gttccagctg tcattggtgc ttgggcgatg
     1681 tacatcttcc aaagtttgtt caacttggtt gttacatcaa attcaggaca agcagctttg
     1741 actatgccaa tcatggctcc actggcagat ttagtaggtg tatccagaca agtagcagtt
     1801 cttgcttatc agcttggttc aggttttatg gacgctttta cacccgtatc agccagcttg
     1861 attggtgtgc taggtgttgc acggatcgaa tgggcaaaat gggcaagatt ccaaatcaag
     1921 atgcaaggat tcctgtttgt tttaggaact attttcatca tgattgccat tgcaataggt
     1981 ctgcagtagt actggagacc tgtaaataaa aaagtaatcc aaacagtaga taaggactcc
     2041 aagaaccgtc attttacggt aaaatcaaag gagtaaagat cctatcgttt ggattatttt
     2101 ttaagatcct atcatcagtt atacttaaag aaagaggaaa aatgtatgaa aatcattcga
     2161 caaattgaag tgtttgcacc agattactta ggaaaaatgg atgtactgat tgccggtggg
     2221 aagattttgg ctgtagaaga acagctagaa ggcggctacg aaggagtgga agtagaagaa
     2281 ctttcaggtg agggaaaagt actaaccccg ggtttcattg actgtcattt ccatatttta
     2341 ggtggaggag gagaaggcgg ttatcaaaat cgaacgccag aagtcacatt gagccaattg
     2401 actacagctg gtgtaacgac agttgtcggt tgtttgggaa cagacggaga aggtagagat
     2461 atgactgcgt tgatcagcaa agcgaaaggt ctggaagcag aaggaatctc cacttatgta
     2521 tacgaaggtt cgtaccgatt gccggtaaaa acagtaacag attctatcat caaagacttt
     2581 ttgacaattg ataaaattat cggaatcgga gaaatcgcag tatccgatca ccgttcttct
     2641 cagccaagtt ttgaagagtt cgctcgtgca gtagcagatg ctcgtgtagg aggcatgtta
     2701 tccggaaaag ctgggatcat caatgtccac ttaggcggag gaaaaagaaa gctagagcta
     2761 ttgacacgaa tcgtagaaga aacagaaatt ccgatcacac aatttttgcc tactcatgca
     2821 aatcgcacac cagaattatt tgaagcttgc gtggcatttg ccaaacgtgg tggcacgatt
     2881 gacttcactg ctagcgaaga tcctgatttt tgggagaaga cagatggtga agttcgcttc
     2941 agcaaagcac tgaaacgatt gatcgaggaa gatgtttctt tagataattt cacgatgacc
     3001 tcagatggac aaggaagctt gccttatttt gatgaaaaca atcatttcct agggttagga
     3061 gtaggaagtg ccaaggcatt gctagtaggg atcaaagaag ctgtgcaaaa agaaagtatc
     3121 cctttggaaa tcgccttacg agctataacc agtaatccag cacgtatatt gaaattggat
     3181 aaaaaaggaa aaatcgaaat tggtgctgat gcagatctgt gtatcttgga taaagaaacg
     3241 cttgatattg atacagttat cgctaaagga gaaataatgg tccaagaaaa agaagtcaaa
     3301 gtatggggga ctttcgaaaa aagtttttaa tcaacagctc taactatctt ttttagaaaa
     3361 cgagcgttct gtttgtctgt atctgttgtt gatgttaccc tgaaggtaga tcaataaaca
     3421 aactaagagg aggaagcaca atggcagatt taaaaggacg ttctaaagat atgaaagata
     3481 aagtagtcgg tgaaggaaaa gaagcatacg gcaaagcaac agacgacaag agcaaagaaa
     3541 cagaagggaa agcacaatca ctaggtgctg acatcaaaga aaaagctcga aatttaggcg
     3601 acgaagtcaa agacaagttt tctcgaaaag acgaaaaaaa ataaaaacca cttattacta
     3661 attgaatcaa gcaaataaaa aatccgaacc atatctgcga gacagatatg gttcggattt
     3721 tttatttgat cattatcctg ttttttaaac ttcgattttt tgaaattttt tattttttag
     3781 ttcaaagact tcatcacagc tttttgctac ttcaatctca tgagtcacga tgataataca
     3841 gcgattttgt tcatgggcga tttcttggaa aagtttgacg atatcttgtg aagttgtttc
     3901 atctagattt ccagtaggct catccgctac gattagttga tgttcacagc agatagcacg
     3961 aacgatagcg actcgctgct gctgacctcc cgaaagtttg gtcacctgtt tttttgctaa
     4021 tatttcatca attcctacac gagccagtcg atctaaagca acttgtcgat aattctcttg
     4081 cttggtctta gtgatttcca ttgctgctat gacgttgtct agagccgata aataaggaag
     4141 caagttgtaa gcttggaaaa taatcgagac atcattttta cgataacttc ttaacccgat
     4201 tttgttcagt gaacgatctt gatagtatac ctttccttct tttggtgcat ctagtccaga
     4261 aataagcgaa agaaaagtcg tttttccaga accacttgct cctaaaatag cgtacatttt
     4321 tccagcttcg aaattcagtt ctacatcttt ataaagcgca tcttgcggat cttgatacca
     4381 aaatcccaat tgttctgtcc gtaacatcaa attcctcctt tagtttaata ggatttttct
     4441 tgggtttaat cttaatatcc ctatagaagc taaaaagaca gaaaacagac tgattccaaa
     4501 tgctactccg gctaatagtc ccaattgtgc aggtttgaca ttgatttcca attcgctgac
     4561 ttgatcagaa acagcaaatg gattattcgt ggttccttgt tgaccatcgc gttgaccgcc
     4621 ttgttcttgt ctgttacctg gcatttgtcc accgccagga gcttgctggt tttgtgtcgt
     4681 ggtttctgtc tgttggtcaa gtagctgctg tcccacggca ttcgcgacga cgtttccgct
     4741 aagcgaagca actcctaatg caagaatcat acaaatagcc acttcggtaa acaattggag
     4801 aatgatttta cttctggatt ctcctaaaga tagcaagacg cctaactcat gccgacgctc
     4861 ccgaatggta atcataatga tcagagtaag tatgataata ccggctacag ccactagtaa
     4921 aacaacattt ttagaaaaac ttgccacgtt gtttaaaggt tccaacattg actgatacat
     4981 ggaatcattt gattgtaagc taaatgtatc tgtgtcgatc agcttctcag cttctttgag
     5041 aaactcatcc attttatctg gatcagatag agtgtaaata gcagaatcta tcgtgttatc
     5101 gtcagatgtc ccatttagtt catttgcaaa agtgtaagaa gtgtaaagtg tattcgacgg
     5161 attcatgaag ttgaatttca tacccatact gccgcttgtt tcgctggatt cataaattcc
     5221 tttgatcgtc atttcataag tggtatcttc atcttcagta ctcgtgattg tgaaagtatc
     5281 tccgactgcc aggtcatttg tttctgctag agtagaatca atgactacat tgtttgtgcc
     5341 tttgtcgctg tcatctattc cttcaccatc cgtgattttt gctgttccat cagaaaaatt
     5401 actattttgt gcagttgctg acacaccact gatttgaaag tctgcttgag tcatttgcgg
     5461 attcccgccc attcctccag caggttcacc gcctgcatca gtagtttctg aactgtcttc
     5521 acttgaatca gaactagtga tggcagttat tccatctttt gccatagctg aggcggaact
     5581 ttcaaaggaa taactcttta cattatctaa ttcagctatt ttttccgcgt ccgttaagga
     5641 aactggtgta agagaaaaac tgcctggatc tggacgttct tcagtggtag aaccgcttga
     5701 agaagtttgt tcctgttttt taaatgaagc ttctcgattg gcacttaaag tcactgttgc
     5761 tccaacttct ttttgtgctt gattcgctgc ttcttctgca gcgcttcgaa tcgtgaggcc
     5821 agctagcaca aaaataagaa tggctgtgaa cacggcaaac aataacaatg aacgtcccca
     5881 ttttactttt gtacttttcc atgcacgttt cataaaattc atattttgca tccctcctag
     5941 tagatgcatt tgataataga caacctatct taaagctagc ttaaatctta tgctgtttgc
     6001 acaccatcaa catgaggttc tgtatatata aaaagtcact atagttattg caaaaaatgt
     6061 ggtaaaatga aaaaagtatg tttcaaaccg acgaagaaga aatgaaagag gaatagcaaa
     6121 agaaatccta gatgctacag agggattttt acgtgcatag ttgttctaac aattgctgat
     6181 actagcgatt tagtttattt gttgtataat aatggggaac agctgaatac ttatataagt
     6241 attctggcta tagcgtttcg caaaagatcc attttgttaa tgaagtatat ccaaaataaa
     6301 atgcccatcc aactgtcaga acgcggatgt gctggtaatt ttcctgctga ttctccataa
     6361 gttgagaaat agtgattttg catatgcttc attggaatgg ctcatatatt tagataaaga
     6421 agaaaatcga catctttaaa cttggatgtc ataagaagag gtgaagtttg tgagtaccat
     6481 taaaattatc ccattaggcg gcgttcgtga aaatggaaaa aacatgtatg tcgcagaagt
     6541 gggcgaggat atttttattt tagattgtgg gctaaaatat cctgaaaatg agttgttggg
     6601 gatcgacgtc gtaatccctg attttacgta tttagaagaa aacattgatc gtgtagctgg
     6661 tgtcttttta acacatggac atgcagatgc gatcggggca cttccttact tgttgtcaaa
     6721 aatttctgta ccagtattcg ggacagagtt gacgatcgag ttagcaaaat tgaatgtcag
     6781 tcgtaatgaa gcagccaaag gatttaagga tttccatgtg gtagatgagc atacagaaat
     6841 agactttggt gaaacagtag tgagcttttt ccgtaccact catacgatac cagattcagt
     6901 ggggatcaat ctgaaaacaa aagaaggtag tatcgtttat acgggagatt ttaaatttga
     6961 ccaaacggct atcccaatgt atcaaacaga ttatgcacga ttggcggaaa tcggcaaaga
     7021 aggagtctta gccttactaa gtgaatcggc aaatgcagaa aatcctcagc ctgtagcttc
     7081 tgaagcccaa atagcagatg aagtatttga tacgatcaaa tattgggaag ggcgtatcat
     7141 cgtcgcttgt gtagcaagta atttgcaacg agtgcaacaa gtattggatg cagcagaaaa
     7201 agcacaacga aaagttgtct tgactggtca agatttcgaa cgaattattc gcacagcgat
     7261 gcgtttagaa aaacttcaat taccaagtga agatctgctt gttacgttga aagacatgaa
     7321 aaactatgat ccagaagaac tgatcatttt agaaactggc cgaatgggtg aacccatcaa
     7381 gtctctgcaa aaaatggcaa acggtacaca ccgtaatgtc aagattgaag aaggagattt
     7441 ggtttatatc actactacgc caagcatcgc gatggaaacc atcgtggcga aaacggaaga
     7501 tatcatttat cgagctggtg gaacagtgaa gttgatctct gagaatatgc gcgtttctgg
     7561 ccatgccaat cctaatgact tgcaattaat gatcaatttg atgaaaccaa catattttgt
     7621 gccagttcaa ggagagtatc gtgaattagc tgctcatgca gatttggctc atgcagtagg
     7681 gatgccatac aaaaatatct atattactgg acgcggagac gtgctggaat ataagaacaa
     7741 gcgtatgagt gttgcaggaa ctacaagtgc agaaaatgta atgattgatg gattaggcat
     7801 aggtgatatc ggaaacattg ttttacgtga tcgaaaaatc ttgtcagaag acgggatttt
     7861 cgtagcggtt gtcactatca gtcggcgaga aaagaaaatc atttcaaaac ctcaaattac
     7921 ttcacgggga ttcgtttacg taaaagctag ccgtgatctg atgaaagaaa gcagcaatat
     7981 gattgaaaca atcgttgaga aacatttaga aagcaatgat tttgagtgga gcaaattaaa
     8041 gcaagacatc cgagatcaat taggccgtta cttatttgaa caaacaaaac gccgcccagt
     8101 gatcttgcca gtcattatgg aagctactca acgtcgaaat aaaaaataaa caaaaagagt
     8161 aagatgaacc tctctataga gtgctcgtct tgctcttttt ttgcgaaaaa caaat
//
LOCUS       DAZKLD010000129         8096 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.129, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000129 DAZKLD010000000
VERSION     DAZKLD010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8096)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8096)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8096
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.129"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(58..579)
                     /locus_tag="ACG8KR_002405"
     CDS             complement(58..579)
                     /locus_tag="ACG8KR_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HFQ0201728.1"
                     /translation="MDISVIDATKTNTQKGILYGSSNAPKKMVEFINLSCPYCRQWFE
                     ESYELLEEAVQSGQLQRVIKLFDKEKESLQRGNVMHRYLTISDGQKAIKEIKQIFDTQ
                     DEWKHLSLQEVADFAVDKLKLTEQKDEQLSQAVINEAEQAHIRFVPTVILGKEIFDES
                     ISIEELKELIQTK"
     gene            777..4457
                     /gene="nifJ"
                     /locus_tag="ACG8KR_002406"
     CDS             777..4457
                     /gene="nifJ"
                     /locus_tag="ACG8KR_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HFQ0201729.1"
                     /translation="MRKMKTMDGNTAAAYISYAFTEIAAIYPITPSSTMAELVDQWSA
                     AGKENVFGQPVKVVEMQSEAGAAGVVHGSLKTGALTTTYTASQGLLLMIPNMYKIAGE
                     LLPSVFHVASRAVTTNALNIFGDHGDVMAARQTGFAMLAESSVQEVMDLAPVAHLASI
                     EGEVPFLNFFDGFRTSHEIQKIEVLEYEELAPLINQEKLASFRKRGMNPNHPTVSGTN
                     QNPDIHFQQRETINPYYDKLPEIVQKYMKEINRLRGTSYDLVTYYGADDAEEVIVSMG
                     SVAQTIEQTIDYLNANGRKTGFLNIHLYRPFPVETFVQKLPHTVKTIAVLDRSKEPGA
                     GGEPLLLDVQSALYDSELRPAVIGGRYGLGSKDVTPDQISAVFDELKKDPSIRKKRFT
                     IGIVDDVTYQSLEPKESLDLTEPQTFQAKFWGFGSDGTVGANKSAIKIIGDHTDKYAQ
                     GYFYYDSKKSGGLTVSHLRFGDTPIRSAYLVEHADLVACHTPAYLHSYDLVKGLKPGG
                     IFLLNTLWSDEQLETHLPLKLKRYLAENNIRFYTINAMRLAQEVGLGRRINTAMETAF
                     FKLADIIPFDEVLPLLKEEALKSYGHKSMAIVEKNIQAIDRTTELLHQVAIPDHWRTL
                     EMPEKINKRNTGYIQEILEPVNAQEGNQLSVGTLVANGMTTGEMPLGTASMEKRGVAL
                     EVPEWISDRCTMCNECAFVCPHAAIRPFLADEEEMEEAPEGFIVREMRGADGVKYRIQ
                     VSVEDCTGCGLCVEACPAKEKALVMKPYEEQKEQAVNWAFAMTLRQKANPVKGKNTVL
                     STQFNQPLLEFSGACSGCGETPYVKLLTQMFGDRMMIANATGCSSIWGGASPASPYTT
                     NECGQGPAWSNSLLEDNAEFGYGMLLAAQTRREALASSIKEVLPKVSEELRELMEDWL
                     VHMFESEGTQQRAAKLQAAIESERSIVPELEDIYKRKDLFVKNSQWMIGGDGWAYDIG
                     FGGIDHVLASGADVNILVLDNEVYSNTGGQTSKATPASAIAKFSASGKYASKKDLGMM
                     AMTYGNVYVAQIASGANQMQTIKAFEEAEKYPGPSLIIAYTPCITHGLLGGMKESLKE
                     AKEAVSSGYWSLYRYNPAMVEKNKNPMTLDYKKPDFSAMPDFMRKQVRFSSLENANPN
                     LAKALFEKTIQDAKTRFYNYAALTGELEKIKKRLEPEESIDVPVKTRKEKTTDLEAEE
                     RRAARAERRKKMEQATKEKKEK"
     gene            4646..5533
                     /gene="cdaA"
                     /locus_tag="ACG8KR_002407"
     CDS             4646..5533
                     /gene="cdaA"
                     /locus_tag="ACG8KR_002407"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0006171 - cAMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HFQ0201730.1"
                     /translation="MSFQIGELFNLDYWQQMFSGDLFSLNFLVNILDILVVWYLVYKL
                     IQLLRGTKAIQLLKGVAIFIVIRIVAEIIGLHTLSWLMNQVITYGVIAAIVIFQPEIR
                     RGLEHLGRSSLFKQSKSEQQEDEKMILSFDKAIQYMSKRKIGALITIERHTGLDEYIE
                     TGIALDADITGELLINIFIPNTPLHDGAVIVKEGKIAVASAYLPLSESMLIPKEFGTR
                     HRAAVGISEVSDAITIVVSEETGDVSITLDNELMAGLSQQEYLAILRRELIAEEKTKN
                     KKNVIQSFLDGMTKGGDRK"
     gene            5530..6699
                     /locus_tag="ACG8KR_002408"
     CDS             5530..6699
                     /locus_tag="ACG8KR_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HFQ0201731.1"
                     /translation="MISKEKRTSILYSFIALLFSIALYFNANGQSVPNTLSGNEAYNQ
                     TVTGVPIKISYDSKRYYIHGYENAVTVKLSSANRVQLNAEANEDTRMFRVTADITKLG
                     EGTHEVPLKIQNLSSAVTAKIEPSTITVTVEKKVIKDFDVETLIPSTFTPSGYELDDT
                     SVSPKKVSITTGDKTLAEIKRVVADVDASMVTDDGINSEVPIQALNAAGEVLSIVSDP
                     VQVTVRADSIKPSKSVRLYGIQQGTPAAGVESYDFSFSKLEAEVSGSSDLLASIGDSI
                     AVPINVSGINHRTTRKIEIPVEEGLSIKPESVSVEITPVLQTSSSSSASTSSRTQENT
                     STSRSISSADSGTEPSTTTISSSSESGTSSSTTSTEETSETSESTQMSRTNSSEN"
     gene            6723..8078
                     /gene="glmM"
                     /locus_tag="ACG8KR_002409"
     CDS             6723..8078
                     /gene="glmM"
                     /locus_tag="ACG8KR_002409"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010739004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HFQ0201732.1"
                     /translation="MGKYFGTDGVRGEANKELTPELAFKLGRCGGYVLSQHEEEGKRP
                     RVLVGRDTRISGQMLEEALISGLLSVGIEVFQLGVISTPGVAYLTRLQKASAGVMISA
                     SHNPAQDNGIKFFGADGFKLVDDQELEIEALLDAEVDELPRPSAEGLGTVEEFPEGLL
                     KYSQFLVQTINGDLSGLTICVDAANGATSTSVNRLFADLETDFYTMGTNPDGLNINDG
                     VGSTHPERLAEMVVEKGADAGLAFDGDGDRIIAVDELGNIVDGDKIMFICAKYLAEKN
                     RLKKDTIVTTVMSNLGFHKAVEGIGLKDVVTQVGDRYVVEEMRKNDYNFGGEQSGHMI
                     FLDFNTTGDGMLSGIQLLNIMKQTGKKLSELAAEVTIYPQKLVNIRVSDKNGAMDVPA
                     IKKVIEEAEAEMGSEGRILVRPSGTEPLLRVMAEAPTDEKVNYYVDKIAAVVKEEIGL
                     D"
BASE COUNT         2602 a         1483 c         1837 g         2174 t
ORIGIN      
        1 gtgtttagag cgtgagacaa aagtgttctt tacttttgtc ctacgctcct tattttttta
       61 ttttgtttgg atcaattctt ttaattcctc tattgaaata gattcatcaa agatttcttt
      121 acccaagatg acagtcggaa caaatcgaat atgtgcttgc tcagcttcgt tgatgacagc
      181 ttggctcagc tgttcgtctt tttgttctgt cagctttagt ttatctactg caaaatccgc
      241 tacttcttgg agagaaagat gtttccactc gtcttgagta tcgaatattt gtttgatctc
      301 tttgatcgct ttttgaccat cgctgattgt gagataccgg tgcatcacgt ttcctcgctg
      361 caagctttct ttttctttat cgaacaattt gatcactcgc tgcaattgcc cgctttggac
      421 tgcttcttct aatagctcat aagattcttc aaaccattgt cggcaatacg gacaagatag
      481 gttgatgaat tcgaccattt ttttaggagc attcgatgaa ccataaagga ttcctttttg
      541 tgtatttgtt ttcgttgcat caatcactga tatatccatg ttaaaacacc tctttcgctt
      601 atagtttata catgtctgac aaaaaaaggc aacgatttag actgtttgtc taaaaattag
      661 atgaccaatt caaatagaca tatgtgaaat atttttataa ataaaagaaa aaatggaatg
      721 ttttacgtta taatggaaca gataataaga gagaatatga acaaggatga tgaatcatgc
      781 gaaaaatgaa aacaatggat ggcaatacgg cagccgcata tatttcttat gcatttaccg
      841 agattgctgc aatatatcca ataacaccca gttcgaccat ggctgaatta gtagatcaat
      901 ggtcagcagc aggaaaagaa aatgtattcg gtcaaccagt aaaagtagta gaaatgcaat
      961 ccgaagcagg agcagctggt gtggtccatg gatcattgaa aactggtgca ctgacaacta
     1021 catataccgc ttctcaaggg ctgcttttga tgataccgaa tatgtacaag attgctgggg
     1081 aactactgcc aagtgttttc catgttgcta gtcgggcagt gacgacaaat gcgttgaata
     1141 ttttcggaga tcacggagac gtgatggctg caagacaaac aggatttgct atgttagctg
     1201 aaagcagtgt acaagaagtg atggatttag cacctgttgc ccatcttgct tctattgaag
     1261 gagaagtacc ttttttgaat ttctttgatg gattccgtac gagtcacgag attcaaaaaa
     1321 tcgaagtttt ggaatatgaa gaattagctc ctctgatcaa tcaggagaaa ttagcttcat
     1381 ttcgtaaacg agggatgaat cccaatcatc cgactgttag tggtacgaac cagaatccag
     1441 acatccattt tcaacaacgg gaaacgatca acccctatta tgataaatta cctgaaatcg
     1501 ttcaaaaata tatgaaagag atcaaccgat tacgaggaac ttcttatgac ttggtgactt
     1561 attacggagc agacgatgcg gaagaagtca ttgtttctat gggatcagtt gcacagacga
     1621 ttgaacaaac gattgattac ttgaatgcaa acggacggaa aacaggtttt ttgaatatcc
     1681 atctttatcg tccttttcca gtggaaacat tcgtgcaaaa gttgcctcat acagtaaaaa
     1741 caattgctgt tcttgatcga agtaaagaac caggggcagg tggagaacct ctcttattag
     1801 acgttcaaag tgcgttatat gattctgaac ttcgcccggc tgttattggt ggtcgctacg
     1861 gattggggtc taaagatgtg actcccgacc aaatctcagc agtttttgat gaattgaaaa
     1921 aagacccaag catacgcaaa aaacggttta ccatagggat cgtggatgat gtgacttatc
     1981 aatctttgga accaaaggaa agtctggatt tgacagaacc gcaaacattc caggccaagt
     2041 tttggggatt tggatctgac ggaacagttg gagcaaataa atcagctatc aagattattg
     2101 gagatcacac cgataaatat gcgcaaggct atttttatta tgattcgaaa aaatctggcg
     2161 gtctgactgt ctctcatctt cgttttggag atacgccgat acgttcagcg tatcttgtcg
     2221 agcatgcgga ccttgttgcc tgtcacacac cagcttacct gcatagctat gacttggtca
     2281 aaggattgaa gccaggtggc atttttttat tgaatactct ttggagcgac gaacaattag
     2341 aaacgcatct gcccttaaaa ttaaaacgtt acttggctga aaataacatt agattctata
     2401 cgatcaatgc catgagattg gcccaagaag tcggtttggg acgccggatc aatacggcaa
     2461 tggaaacggc tttttttaaa ttagctgaca tcattccttt tgatgaggtg cttccgctgc
     2521 taaaagaaga agccctgaaa agctatggac ataaatcaat ggctatcgta gaaaaaaaca
     2581 tacaagcaat cgatcggacg accgaactgc ttcatcaagt cgcgatccca gaccattgga
     2641 gaacgttgga gatgcctgaa aaaatcaata aaaggaacac tggttatata caagaaatac
     2701 ttgaaccagt gaatgcacag gaaggaaatc aactctctgt tggtacactt gtagcaaatg
     2761 gaatgacaac tggcgaaatg cctttaggaa cggcttctat ggaaaagcgg ggcgtcgctt
     2821 tagaagtacc tgagtggatt agtgatcgct gtacgatgtg taatgaatgt gcctttgttt
     2881 gtcctcatgc tgccatccgt ccatttttag cagatgaaga ggaaatggaa gaagcgccag
     2941 aaggatttat cgtgagagaa atgcgaggcg cagacggcgt aaaataccgt atccaagttt
     3001 cagtggaaga ctgtacaggt tgtggtctgt gtgtagaggc ttgtcccgca aaagaaaaag
     3061 ctttagtgat gaagccttat gaagaacaga aagaacaagc tgtgaattgg gcttttgcaa
     3121 tgacattaag acaaaaagca aatccagtca aaggaaagaa tactgtcttg tctacacaat
     3181 tcaatcaacc gcttcttgaa ttctcgggcg cttgctcagg ctgtggcgaa acaccttatg
     3241 taaaattatt gacacaaatg ttcggtgatc gaatgatgat cgcgaatgcg acaggctgtt
     3301 cttctatttg gggcggtgct tctcctgctt ctccttatac gaccaatgaa tgtggtcaag
     3361 ggcctgcatg gtcaaattct ttattggaag ataatgctga atttggttat ggtatgcttc
     3421 tagcggcaca gactcgtcgc gaggctttgg caagttcaat caaagaagta ttgccaaaag
     3481 tatcagaaga actacgagaa ttgatggaag actggttggt acacatgttt gagagcgaag
     3541 gaacgcagca aagagcagca aaactgcaag ctgcgatcga aagcgaacga tcaattgtcc
     3601 ctgaactgga ggatatatac aagagaaagg atctattcgt aaaaaacagc caatggatga
     3661 ttggtggaga cggatgggct tacgacattg gttttggcgg gatcgatcat gtacttgcca
     3721 gcggagcaga cgtgaatatt cttgttttgg ataacgaagt ctattccaat actggcggtc
     3781 aaacctcaaa agcaacacca gcttcggcaa tcgctaaatt ttcggctagt ggaaagtatg
     3841 cttcaaagaa agatctagga atgatggcga tgacatatgg aaatgtttat gtggcgcaaa
     3901 ttgcttcagg tgccaatcaa atgcagacga taaaggcttt tgaagaagca gaaaagtatc
     3961 ctggaccttc tttaatcatt gcctatacgc cttgtatcac acatggtttg cttggtggaa
     4021 tgaaagaatc gcttaaagaa gcaaaagaag ctgtttcttc tggctattgg tcattatatc
     4081 gatataaccc tgcaatggtc gaaaaaaaca agaacccaat gactttggac tacaaaaaac
     4141 ctgatttctc tgctatgcct gatttcatga ggaaacaagt tcgcttttcc tcgctagaga
     4201 acgcaaatcc aaatcttgca aaagctttat ttgaaaagac tatccaagac gctaaaacac
     4261 gtttttacaa ctatgctgca ctgacgggag aattagaaaa aatcaagaaa cgacttgaac
     4321 cagaggaatc tatcgatgta cccgttaaga ctagaaaaga aaaaacgacg gaccttgaag
     4381 cagaagaaag aagagctgcc agagcagaac gacgtaaaaa aatggaacaa gctacaaaag
     4441 aaaaaaagga aaaataacag aaaaaatata atgaataatt ctctgaagtt taaaaatatt
     4501 tattgcagag aaaaataaaa cgcatacttg caattttcct gaaaattatg cgtttttctc
     4561 aagaaaaata cgagcagatc agcctctaaa attaaaacat atgatatact agagaggaaa
     4621 tctcaaaaga gaagagaggc tttttatgtc atttcaaatc ggggaacttt ttaatttgga
     4681 ctattggcag caaatgtttt ccggtgactt gttctctctg aattttttgg tgaacatact
     4741 tgatattctg gttgtctggt atcttgtata caaactgatc caactgctgc gaggcacaaa
     4801 agcgattcag cttctgaaag gtgtagctat ttttattgtt atccgaatcg tagcggaaat
     4861 cattggtctg cacaccttgt cttggctgat gaaccaagtc attacgtacg gggtgatagc
     4921 tgctatcgtg atttttcagc cggaaatccg gagaggactg gaacatcttg gacgcagttc
     4981 gcttttcaaa caatcaaaaa gcgaacagca ggaagatgag aagatgatct tgtcttttga
     5041 caaagccatc caatatatgt ccaagcgaaa aatcggtgcg ctaattacta tcgaacggca
     5101 tactggatta gatgagtata ttgagacagg aattgcatta gatgcagata tcactggaga
     5161 gctgctgatc aatatcttta ttcctaatac accgctgcat gacggtgcag ttattgtaaa
     5221 agaaggaaag atcgcagtag caagcgccta tcttcctcta tcagaaagta tgttgattcc
     5281 aaaagaattc gggactcgac accgggcagc tgtcggtata agcgaagtca gcgacgcgat
     5341 taccatcgtt gtctctgaag aaactgggga tgtcagcatt acattagata atgaactgat
     5401 ggctggctta tcccagcaag aatatttggc aattttgcgt agagaactga ttgctgaaga
     5461 gaaaacaaaa aacaagaaaa acgtgattca atctttccta gacggtatga ctaagggagg
     5521 ggatcgcaaa tgatctcaaa agagaaacgg acaagcattc tttacagttt tattgcattg
     5581 ctgttcagta ttgcgctgta cttcaatgca aatggccagt ccgttccaaa tacgctttct
     5641 ggaaatgaag cgtataatca aacagttact ggggtgccaa tcaagatttc ttatgattca
     5701 aagcgctact atatccatgg atatgaaaat gcagtgactg tcaaactttc cagtgcaaac
     5761 cgagttcagt tgaatgcaga ggcaaacgaa gatacacgga tgttccgcgt cacagcagat
     5821 atcactaagc tgggcgaagg aacacatgag gttccgctaa aaattcaaaa tttgagcagt
     5881 gctgtcacag caaagatcga accatcgact attacagtaa cggttgagaa aaaggtaata
     5941 aaagatttcg atgtggaaac gttgattcct tcaacgttca caccatctgg atatgaactt
     6001 gatgatactt ccgttagtcc taaaaaagtc tctatcacta caggtgacaa aacattggca
     6061 gaaatcaaac gagtagtagc tgatgtggac gcttcgatgg tcacagatga tgggattaat
     6121 agtgaggtcc caatacaggc gctgaatgca gcaggggaag tattaagtat cgtctctgat
     6181 ccagtgcaag taacagtgag agctgattcg atcaagccaa gcaagtcggt acgactttat
     6241 gggatacagc aaggaacacc agctgccggg gtagaaagct atgatttcag tttttcaaaa
     6301 ctggaagcag aagtaagcgg ctctagcgat ttgcttgcat cgatcggtga cagtattgct
     6361 gtaccgatca atgtctcagg tatcaatcat cgaacaactc gtaaaattga aataccagta
     6421 gaagaggggt taagtatcaa gcccgaatca gttagtgtgg agattacacc cgtgttgcaa
     6481 acgtcaagca gtagctctgc ttcgacgagc agtcggacac aagaaaatac aagtacgagt
     6541 agaagtattt catctgctga ttcaggaacg gaaccatcga caacaaccat ttcttccagt
     6601 tcggaatctg ggacaagcag ctcgaccaca agtacagaag aaacatcaga aacaagcgaa
     6661 tcaacacaaa tgagcagaac aaattcctca gaaaattaag ttaaaggagt aattttacaa
     6721 ctatgggtaa atattttgga acagacggcg tacgcggtga agccaacaag gaacttacgc
     6781 cagaacttgc atttaagctg ggacggtgcg gaggttatgt cctaagccag catgaagaag
     6841 aaggaaaacg cccacgcgtg ttagtagggc gggatacaag aatctcaggt caaatgttag
     6901 aagaagcgct gatttcagga cttttatcgg tgggtatcga agtgttccag ctaggtgtga
     6961 tctctacacc aggtgttgct tatctgacac gtttgcaaaa agcaagcgca ggggtcatga
     7021 tttctgcttc tcataatcca gcacaagata atggaatcaa atttttcggt gcagatggtt
     7081 ttaaactagt agatgaccaa gagctagaaa ttgaagcatt attagatgct gaagtggatg
     7141 aattgcctcg tccatcagct gaaggattgg gaactgtaga agaatttcca gaaggcttat
     7201 tgaaatattc tcaattcctt gtacaaacga tcaacggtga cctttccggt ttgaccattt
     7261 gtgtcgatgc agcaaacgga gcaacttcaa caagtgttaa tcgtttgttt gccgatcttg
     7321 aaacagattt ttataccatg ggcacaaatc cagacgggtt aaatatcaat gatggcgtag
     7381 gctctaccca tccagaacgt ttagctgaga tggtcgttga aaaaggagca gatgctggac
     7441 tagctttcga tggcgatggc gatcgtatta ttgcggtgga tgaacttgga aacattgtcg
     7501 acggtgataa aatcatgttt atctgtgcta aatacttggc agaaaaaaat cgattgaaaa
     7561 aagatacgat cgttacgact gtcatgagta acttaggatt ccacaaagcg gtggaaggaa
     7621 tcggtttgaa agatgtcgtt acacaagttg gtgatcgtta cgttgtagaa gaaatgcgca
     7681 aaaacgatta taactttggc ggcgagcagt ctgggcacat gattttctta gattttaaca
     7741 caactggaga tgggatgctt tcaggaattc agttactgaa tatcatgaaa cagacaggta
     7801 agaaattatc agagcttgca gcagaggtaa caatctatcc gcaaaaatta gtgaatatcc
     7861 gagtaagtga taaaaacggt gcgatggatg ttccggcaat caaaaaagtg attgaagagg
     7921 cagaagctga aatgggatca gaaggaagaa tcctagttcg tccttccggt acagaaccat
     7981 tacttcgagt gatggcagaa gcgcctactg atgagaaagt taattactat gtcgataaaa
     8041 tcgcggcagt agtcaaagaa gaaatcggac tggattaaga gatattttca taaaaa
//
LOCUS       DAZKLD010000130         8062 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.130, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000130 DAZKLD010000000
VERSION     DAZKLD010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8062)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8062)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8062
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.130"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..514
                     /locus_tag="ACG8KR_002410"
     CDS             <1..514
                     /locus_tag="ACG8KR_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CatA-like O-acetyltransferase"
                     /protein_id="HFQ0201733.1"
                     /translation="GYEIYPSLIYAIMEVVNKNKVFRTGINSENKLGYWDKLNPLYTV
                     FNKQTEKFTNIWTESDKNFISFYNNYKNDLLEYKDKEEMFPKKPIPENTIPISMIPWI
                     DFSSFNLNIGNNSSFLLPIITIGKFYSENNKIYIPVALQLHHSVCDGYHASLFMNEFQ
                     DIIHRVDDWI"
     gene            complement(1207..2145)
                     /locus_tag="ACG8KR_002411"
                     /pseudo
     CDS             complement(1207..2145)
                     /locus_tag="ACG8KR_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
     gene            complement(2145..2762)
                     /locus_tag="ACG8KR_002412"
     CDS             complement(2145..2762)
                     /locus_tag="ACG8KR_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFQ0201734.1"
                     /translation="MAKIGYARVSSKEQNLDRQLQALQGVSKVFSDKLSGQSVERPQL
                     QAMLNYIREGDIVIVTELDRLGRNNKELTELMNQIQIKGATLEVLNLPSMNGIEDENL
                     RRLINSLVIELYKYQAESERKKIKERQAQGIEIAKKKGKFKGRQHKFKENDPRLKHAF
                     DLFLNGLSDKEVEEQTGINRRTFRRYRARYNVTVDQRKNNEKRDS"
     gene            complement(2776..2946)
                     /locus_tag="ACG8KR_002413"
     CDS             complement(2776..2946)
                     /locus_tag="ACG8KR_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000713595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0201735.1"
                     /translation="MKKFIYRVLENDEVVAIFNEQQYAQDFIAYEKTISDKQFEIEKV
                     DIADWLLQPREF"
     gene            complement(3294..4784)
                     /locus_tag="ACG8KR_002414"
     CDS             complement(3294..4784)
                     /locus_tag="ACG8KR_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015543625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase C-terminal domain-containing protein"
                     /protein_id="HFQ0201736.1"
                     /translation="MNIPFVVETVLHDGLLKYKFKNSKIRSITTKPGKSKGAIFAYRS
                     KSSMIGGRGVVLTSEEAIQENQDTFTHWTPNVYRYGTYADENRSYTKGHSENNLRQIN
                     TFFIDFDIHTAKETISASDILTTAIDLGFMPTMIIKSDKGYQAYFVLETPVYVTSKSE
                     FKSVKAAKIISQNIREYFGKSLPVDLTCNHFGIARIPRTDNVEFFDPNYRYSFKEWQD
                     WSFKQTDNKGFTRSSLTVLSGTEGKKQVDEPWFNLLLHETKFSGEKGLIGRNNVMFTL
                     SLAYFSSGYSIETCEYNMFEFNNRLDQPLEEKEVIKIVRSAYSENYQGANREYITILC
                     KAWVSSDLTSKDLFVRQGWFKFKKKRSERQRVHLSEWKEDLMAYISEKSDVYKPYLVT
                     TKKEIREVLGIPERTLDKLLKVLKANQEIFFKIKPGRNGGIQLASVKSLLLSIIKVKK
                     EEKESYIKALTNSFDLEHTFIQETLNKLAERPKTDTQLDLFSYDTG"
     gene            complement(4827..5126)
                     /locus_tag="ACG8KR_002415"
     CDS             complement(4827..5126)
                     /locus_tag="ACG8KR_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0201737.1"
                     /translation="MNHDECKTYIKNSLLEIRKLANIYTLETFKKELEKRNIYLETKS
                     DKYFSSEGEDYIYKLIENNKIIYSISGKKLTYKGKKSFSKHAILKQLNEKANQVN"
     gene            complement(5492..5776)
                     /locus_tag="ACG8KR_002416"
     CDS             complement(5492..5776)
                     /locus_tag="ACG8KR_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFQ0201738.1"
                     /translation="MELAFRESLKKMRGTKSKEKFSQELEMSRSNYSRIESGKSDPTI
                     KTLEQIAKLTNSTLVVDLIPNEPTEPEPETESEQVTLDLEMEEEKSNDFV"
     gene            complement(6295..6780)
                     /gene="vanZ-A"
                     /locus_tag="ACG8KR_002417"
     CDS             complement(6295..6780)
                     /gene="vanZ-A"
                     /locus_tag="ACG8KR_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance protein VanZ-A"
                     /protein_id="HFQ0201739.1"
                     /translation="MGKILSRGLLALYLVTLIWLVLFKLQYNILSVFNYHQRSLNLTP
                     FTATGNFREMIDNVIIFIPFGLLLNVNFKEIGFLPKFAFVLVLSLTFEIIQFIFAIGA
                     TDITDVITNTVGGFLGLKLYGLSNKHMNQKKLDRVIIFVGILLLVLLLVYRTHLRINY
                     V"
     gene            complement(6933..7844)
                     /locus_tag="ACG8KR_002418"
     CDS             complement(6933..7844)
                     /locus_tag="ACG8KR_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase VanY-A"
                     /protein_id="HFQ0201740.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYEPWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHTNSRR"
BASE COUNT         2433 a         1456 c         1205 g         2968 t
ORIGIN      
        1 aggatatgaa atttatccct ctttaattta tgcaattatg gaagttgtaa ataaaaataa
       61 agtgtttaga acaggaatta atagtgagaa taaattaggt tattgggata agttaaatcc
      121 tttgtataca gtttttaata agcaaactga aaaatttact aacatttgga ctgaatctga
      181 taaaaacttc atttcttttt ataataatta taaaaatgac ttgcttgaat ataaagataa
      241 agaagaaatg tttcctaaaa aaccgatacc tgaaaacacc ataccgattt caatgattcc
      301 ttggattgat tttagttcat ttaatttaaa tattggtaac aatagcagct ttttattgcc
      361 tattattacg ataggtaaat tttatagtga gaataataaa atttatatac cagttgctct
      421 gcaacttcat cattctgtat gtgatggtta ccatgcttca ctatttatga atgaatttca
      481 agatataatt catagggtag atgattggat ttagttttta gattttgaaa gtgaatttaa
      541 ttttatacac gtaagtgatc ataaaattta tgaacgtata acaaccacat tttttggttg
      601 cttgtggttt tgattttgaa tttggttttg aacttatgga ctgatttatt cagtccattt
      661 tttgtgcttg cacaaaaact agcctcgcag agcacacgca ttaatgactt atgaaacgta
      721 gtaaataagt ctagtgtgtt atactttact tggaagatgc accgaataaa aaatattgaa
      781 gaacaactag caaaagattt taaagagtta ttttatttta agtctttata acatgagtga
      841 agcgaatttt taaatttcga tagaaatttt tacatcaaaa agccccctgt caaaattgac
      901 gaagggggtt ttttggcgca cgcttttcgt tagaaatata caagattgaa aatcgtgtat
      961 aagtgcgccc tttgttttga acttagcacg ttacatcaat tttttaaaat gatgtataag
     1021 tgcgcccttt taaattttga gtgattatat tttttgagtt agaaaaaggg attgggaaaa
     1081 tttcccaaaa taatttaaaa aataagcaaa aattttcgat agagaatgtg ctattttttg
     1141 tcaaaggtgt ataccttgac tgtgcttgct gttacattaa gtttattttt aagttattaa
     1201 aaaagaaata gcttttaaag tttggctcgc tgtcgcttta taaagctgat tgacttttga
     1261 ttgcaaacta cttaaagaaa acaaactcgg actattcgtt ttcttctctt tggtttgaac
     1321 atcagcaatt atcccctctt gattgcctat tttagcttgt ttagaagaaa caaaagctaa
     1381 aagctcctct tgggttttaa aacgctgtgt ggggcttaga acgcccttaa acgacccttg
     1441 gtttactttt atactagctt ccacctcgaa aaaaggttct tttttaaaat tctctatggc
     1501 ttcctggcgc tgaaaaataa ggtataaggt gggcgtttga acacgtccta gtgaaaatgt
     1561 accttgtacg cccttctgtt gtaaatttaa cgtatacaaa gggcttgcgt tcatgccgat
     1621 caaccaatcg gcaatttggc gtgtttgcgc ttcttgataa aagggatagt aattcattcc
     1681 aggttgcaaa ttttgaaaac cgcttcggat tacatctttt tctaagctat tgatccatag
     1741 tcttttaaat gttttatctt ttgaaaaggc atttgcttta tggataatcg accaggcgat
     1801 attttcacct tctctgtcgc tatctgttgc aacaataatt gtatttgcct ttttgagaag
     1861 ttctgcaaca attttaaact gctttccctt atcttttgca acttcaaaat cgtatcgatc
     1921 aggaaaaatc ggcaaagatt caagtttcca attttgccac ttttcgtcat aatgacctgg
     1981 ttctgctaat tccactaaat gcccaaaacc aaaggtgata aacgtttcat ctgtaaatag
     2041 tgggtctttg atctcaaaat aaccgtcttt tttggtgctt tgttttaaag cacttgcgta
     2101 ggctaatgcc tggcttggtt tttcagctaa aataaccgta ctcattaact atccctcttt
     2161 tcattgtttt ttctttgatc gactgtcacg ttatatcttg ctcgatacct tctaaacgtt
     2221 cggcgattga ttccagtttg ttcttcaact tctttatcgg ataaaccatt caaaaacaaa
     2281 tcgaaagcat gctttaaacg tggatcattt tctttaaatt tatgctgacg acctttgaat
     2341 ttgccttttt tcttagcaat ttcgattcct tgtgcctgac gttccttaat tttttttcgt
     2401 tctgattctg cttgatactt gtacaattca atgacaaggc tattaatcaa acgccttaaa
     2461 ttttcatctt caataccatt cattgagggt aaatttaaga cttccagggt tgccccctta
     2521 atttgaattt gattcatcaa ttctgttaat tctttattat ttcgtcctaa tcgatctaat
     2581 tcagtaacaa taacaatatc cccttcacga atatagttaa gcatagcttg taattgtggg
     2641 cgttcgaccg attgaccgct taatttgtct gaaaagacct tagaaacgcc ctgtaacgct
     2701 tgtaattgcc gatctaagtt ctgttctttg ctactgacac gtgcataacc aattttagcc
     2761 attttcaacc aacctctaaa attctctcgg ttgcaataac caatcagcaa tatctacttt
     2821 ttcaatttca aattgcttat cagaaattgt cttttcgtaa gcgataaaat cttgcgcata
     2881 ttgttgctca ttaaaaatag ccaccacttc gtcattttct aaaactcgat aaataaattt
     2941 tttcatttta ctcctcctat tatgcccaac ttaaatgacc tattcaccaa gtcaattata
     3001 ctgctaaaat catattagga caaataggta tactctaatg acctataaaa gatagaaaat
     3061 taaaaaatca agtgttcgct tcgctctcac tgcccctcga cgttttagta gcctttccct
     3121 cacttcgttc agtccaagcc aactaaaagt tttcgggcta ctctctcctt ctccccctaa
     3181 taattaatta aaatcttact ctgtatattt ctgctaatca ttcgctaaac agcaaagaaa
     3241 aaacaaacac gtatcataga tataaatgta atggcatagt gcgggtttta ttttcagcct
     3301 gtatcatagc taaacaaatc gagttgtgtg tccgttttag ggcgttctgc tagcttgttt
     3361 aaagtctctt gaatgaatgt atgctctaag tcaaaagaat ttgtcagcgc ctttatatag
     3421 ctttcttttt cttctttttt tactttaatg atcgatagca acaatgattt aacactagca
     3481 agttgaatgc caccatttct tcctggttta atcttaaaga aaatttcctg attcgccttc
     3541 agtaccttca gcaatttatc taatgtccgt tcaggaatgc ctagcacttc tctaatctct
     3601 tttttggtcg tcactaaata aggcttgtat acatcgcttt tttcgctaat ataagccatt
     3661 aaatcttctt tccattctga caaatgaaca cgttgacgtt cgcttctttt tttcttgaat
     3721 ttaaaccacc cttgacggac aaataaatct ttactggtta aatcacttga tacccaagct
     3781 ttgcaaagaa tggtaatgta ttccctatta gccccttgat agttttctga ataggcactt
     3841 ctaacaattt tgattacttc tttttcttct aagggttgat ctaatcgatt attaaactca
     3901 aacatattat attcgcacgt ttcgattgaa tagcctgaac taaagtaggc taaagagagg
     3961 gtaaacatga cgttattacg ccctattaaa cccttttctc ctgaaaattt cgtttcgtgc
     4021 aataagagat taaaccaggg ttcatctact tgttttttgc cttctgtacc gcttaaaacc
     4081 gttagacttg aacgagtaaa gcccttatta tctgtttgtt tgaaagacca atcttgccat
     4141 tctttgaaag aataacggta attaggatca aaaaattcta cattgtccgt tcttggtatg
     4201 cgagcaatac caaaatgatt acacgttaga tcaactggca aagactttcc aaaatattct
     4261 cggatatttt gcgaaattat tttggctgct ttgacagatt taaattctga ttttgaagtc
     4321 acatagactg gcgtttctaa aacaaaatat gcttgataac ctttatcaga tttgataatc
     4381 atagtaggca taaaacctaa atcaatagcg gttgttaaaa tatcgcttgc tgaaatagtt
     4441 tcttttgccg tgtgaatatc aaaatcaata aagaaggtat tgatttgtct taaattgttt
     4501 tcagaatgtc ctttcgtgta tgaacggttt tcgtctgcat acgttccata acgataaacg
     4561 ttgggtgtcc aatgtgtaaa tgtatcttga ttttcttgaa tcgcttcctc ggaagtcaga
     4621 acaacaccac gaccgccaat catgcttgat tttgagcgat acgcaaaaat agcccctttg
     4681 cttttacctg gcttggtagt gattgagcga attttactat ttttaaattt gtactttaac
     4741 aagccgtcat gaagcacagt ttctacaaca aaagggatat tcattcagct gttctccttt
     4801 cctataaatc ctataaaata ggttgtttaa ttaacttggt ttgctttttc attcaactgt
     4861 ttcaatattg catgttttga aaaagatttt tttcctttat aagtcaattt ttttccacta
     4921 atcgaataaa ttattttgtt attttctatt aacttatata tataatcttc cccctccgaa
     4981 gaaaaatact tatctgattt tgtttctaag tagatatttc tcttttctaa ctctttctta
     5041 aacgtttcta gtgtatagat atttgctaat tttcttatct ccaataaact attttttata
     5101 taagttttac attcatcatg attcatacaa actccacctt ctataaatga atacaaaaaa
     5161 agcaatcaaa cgatttccga ttgattgctt aacaattctt aaattcagta gcttagatac
     5221 ttgaaaactc tctgatttcc ctatataatg atagtacggt tatataccgt cttcaaacaa
     5281 agttaattaa ataacttctt acgagggaag agttcatctg actaactgat aagcgttggt
     5341 ttggcaatct tatcgggcta tgcatttata aaatgtcgtc aaacatttta taaatgtgtc
     5401 atggctcttt tttcgtttct attcagttcg ttgtttcgtt atatctagta taccgctttt
     5461 aaaaaaaata agcaacgatt tcgtgcatta ttcacacgaa gtcattgctt ttttcttctt
     5521 ccatttctaa atccaatgtt acttgttctg attctgtttc tggttctggt tctgttggct
     5581 catttgggat taaatccact actagcgttg agttagttaa ctttgcaatt tgttctagtg
     5641 tttttatggt tggatctgat tttcctgatt ctattcgtga ataatttgat ctactcattt
     5701 ctaattcttg ggagaatttt tcttttgatt tggtacctct catctttttt aagctttctc
     5761 taaatgctag ttccaagttt ctcccccctc tttttgtgta ataaaattca cgctttattt
     5821 aaattatagc ttttaatatc ttaataaaca agtgattaat cacttgtgat ttgtgattat
     5881 tccaaaagta ggtaatttca ccaacctttt tcaaaagttt ttatcgacga ttattggact
     5941 ggatcgtgtt tcctaaagcg attctaagcg catgtttaat tttaaagtgg gtaattctct
     6001 ttaaaacgtc ctacaaagat tcaaaaaaag catggctgct agtgggggta ccgtcaggaa
     6061 aatgcggatt tacaacgtta aggaaattta ttaaagagac caattcctca gcactaaaat
     6121 tgataattgg gctttctttg ttactatagt gttaggtaag ctttttactt tatcaccggt
     6181 tttcccctca cttcacacct acgggcgagt ttccccacat cccgttttcc atctaatcca
     6241 attcattcaa tatctttatg tgtatgaaag atcagattgc ttgatttaga catcttacac
     6301 gtaatttatt cttaaatggg tacggtaaac gagcaataat acgagcaaaa gtatacctac
     6361 aaaaataata actctgtcta attttttttg attcatatgc ttattgctta aaccatataa
     6421 tttcagtcca agaaagcctc caacagtatt tgtaattaca tctgttatgt ctgtcgctcc
     6481 aatagcgaag ataaattgaa ttatttcaaa agtaagactt aaaaccagta caaaagcaaa
     6541 cttaggtaaa aatccgattt ctttaaaatt gacattcaaa agcaagccaa atggaataaa
     6601 gattataaca ttatctatca tctctctgaa attcccagta gcagtaaatg gagtcaagtt
     6661 aagacttctt tgatgataat taaatactga taaaatattg tattgtaatt tgaataacac
     6721 taaccagatt agtgtcacta aatataaagc tagcaatcct ctagataata tttttcccaa
     6781 tataatttcc tctaattctt ttgtttaagt tgtatcatta gtatagcaat gtcctagtta
     6841 acgtgggttt taaattaaaa agctaacgtt accccaaaag tgctagtaca ataatgctgt
     6901 tcattaattt cgttggtttc attccgccat ccttacctcc ttgaattagt atgtgttgat
     6961 ccgggaaaca ctgtcacaat tacaccgtct atattgtttc ctgatatctc ataacgaaga
     7021 ttagtcggca catgaatggt ggtattttta gtaacaggat aataaaagat ctcatatttt
     7081 tccccattta cactaacaga aatggttttt tcttctttta ggtaatccat atattcctcg
     7141 agaacgaaat tcttttcttt cataatcgca ctatgtggta aaccaacata gcgaatatgc
     7201 catggttcat attgaattcc tgttaactct gttttgtcct ctggataacg taaaatgaac
     7261 ccgtatttcc aagcattttc ttctatccac tttccttcag gggctcgttc cattttcgtc
     7321 aagcttgatc ctacatctag tgataaacct gaattatgct cactataacc tgctggtaag
     7381 gcatactcag cccccatttc ttggtaaagc acactttgct catcaaagtc tcgatagcca
     7441 ctattaataa taaaatgact aacgccaccc tttacagcat cattgaccat ctctgaaaat
     7501 ttttgtgcta tttcttttga catataaata ttactatcaa gcaacccgta tccatttatt
     7561 aattcgtcat gtttagataa attcacgata tctgacttca cactttcttg gcgaacagga
     7621 tatttactat tgattaatag cagatttcct tgataaacct gttcttctgt aattgttttc
     7681 tcttgggtat tttcagaagt cccactattt tctaaatgtt ctttgggatt ttgatcataa
     7741 ttttgaaatt cgactttttc ctgagaaaac agtgcttcat taacgtagtc ataacctaag
     7801 tatattaaga ataacaataa caataaaaaa aacaacttct tcattttcag tctcctcctt
     7861 aactatatag ttggaagtat gaaatatttc tagaatcttc cccttaaacg gcacaatctc
     7921 gtttgagatt gtgccgtttt gccccgtaaa ttacagtcaa ttatttcgcc ctactggatt
     7981 tactattatc accaatgtag atgaattttc ataagtgata cccatgctga atttaacgag
     8041 gctgaactgc ctgttgacaa tg
//
LOCUS       DAZKLD010000131         7983 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.131, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000131 DAZKLD010000000
VERSION     DAZKLD010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7983)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7983)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7983
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.131"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            476..1339
                     /locus_tag="ACG8KR_002419"
     CDS             476..1339
                     /locus_tag="ACG8KR_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFQ0201741.1"
                     /translation="MYQIVTDSCCDLPYHVLAKEKVDFLSMNLQLNDQELVDDLGKTF
                     NYDRYMNQLKNGAMPTTSQINVGRYLEFFRSYAEKQIPVLYVAFSSGLSGSYSSALQA
                     VELLKEEYEDPEIYVFDTKAASIGEGLLVLEAVRLKKEGKSIEETLEWLESNYLKVHS
                     WVTVDDLKYLERGGRISKASATLGSLLSVKPIITVDKEGRLTNVDKVRGRNKSIQTIV
                     NETVKTIVAPLDQIVYVAYAGDLESAEKAKRLLEEQIKMKDVKLYPLGPTIASHTGYG
                     CIAIFSMGISR"
     gene            complement(1468..3090)
                     /locus_tag="ACG8KR_002420"
     CDS             complement(1468..3090)
                     /locus_tag="ACG8KR_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFQ0201742.1"
                     /translation="MITVNDVSLHFSDRKLFDDVNIKFTPGNCYGLIGANGAGKSTFL
                     KVLSGDLQPSTGSVTMGPDERMAVLKQNHYDYEDYTVLETVIMGHKRLYEVLKEKDAI
                     YMKEDFTDEDGIRAAELEGEFAELNGWEAEPEAAVLLQGLNIPEELHHQQMSELTGGQ
                     KVKVLLAQTLFGKPDVLLLDEPTNGLDIQSINWLEEFLINFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWLESSQLAAKLQANANAKKEEQIKELQEFIARFSANA
                     SKSKQATSRKKMLEKITLDDIQPSSRRYPFVGFKPEREIGNDLLQVENVSVTIDGKKI
                     LDDISFNLSKEDKVAFVAENDITTTTLFKVIMGEITPDTGTVRWGVTTSNSYLPKDTT
                     KEFDTDLTILDWLRQYASKEEDDNTFLRSFLGRMLFSGEEVLKPVNVLSGGEKVRCML
                     SKMMLSKANVLVLDDPTNHLDLESITALNDGLMAFTGSILFASHDHQFIQTLANRIIA
                     VSDKGVIDRADTTYDEFLENADIQKQLNELWA"
     gene            3339..3860
                     /locus_tag="ACG8KR_002421"
     CDS             3339..3860
                     /locus_tag="ACG8KR_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HdeD family acid-resistance protein"
                     /protein_id="HFQ0201743.1"
                     /translation="MDEKRTIDWGSLILGILFVLVSLLSFQDPVGNLVAIVVVFAIFA
                     FIKGIFELFVRNRLKELTGYKGKMPLIIGIIDILVGVFFLFNIGAGVAALPFVFAVWF
                     IADSVLALFTADLARGVSEGYYWFTIIVNILGILLGIFLLFNPISSALTLSFLVGFYF
                     MLFGITHIVYAFR"
     gene            complement(4046..5776)
                     /gene="spxB"
                     /locus_tag="ACG8KR_002422"
     CDS             complement(4046..5776)
                     /gene="spxB"
                     /locus_tag="ACG8KR_002422"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047112 - pyruvate oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HFQ0201744.1"
                     /translation="MSKINAGIAMVKVLESWGIDHIYGIPGGSFNSIMDALYQEKTEI
                     NYIQVRHEEVGALAAAADAKLTGKVGAVFGSAGPGATHLINGLYDAQMDHVPLVAFLG
                     QVASTSMNYNSFQELNENPIFADVSVYNRTVMTPQSLPHVVDEAIKAAYEHKGVAIVT
                     IPVDLGFEEIDEQPFSTAHTHKTSVILPEEKDLLAALPYLEKAKKPVLYVGQGTRNGF
                     PQIKEFSEHFSVPVVASVLAKGIIPDDYENFLGFAGRVATKPGNEALAEADLILFVGS
                     DFPFGRAFFNPTAEFIQIDIDASKFGRRHETSLSILGDANTALQRLVELGDARPADSW
                     YLANQENKEKWVNWLKSFEDREEEPIRPEAVYKEINRIAEDDAIFVTDVGNTTIHSIR
                     LLNMNGKQKHTTSGWFATMGNGVPGGIAAQLSYPEKQVFTLSGDGGFAMVMQDIITQV
                     KYQLPIINVVFSNDSFGFIEAEQEDTEQKKFGVFLEDADFGKVGEALGANSFTVTEYS
                     QLRFAFDAAAKSDRPVVIDVKINNKRPLPVEDLHLDPRKYSEEEIQAFKEKYEVHDLP
                     VLNELYEKRL"
     gene            5941..6759
                     /locus_tag="ACG8KR_002423"
     CDS             5941..6759
                     /locus_tag="ACG8KR_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HFQ0201745.1"
                     /translation="MVEWLLRLEPWQQALAGTAFTYFMTALGAGLVFFFKEIKKEVLN
                     LMLGFASGVMIAASFWSLLDPAITKAEENGDIAWLVVSIGFGLGGVFLYMADKTLPHM
                     HFGPQHEAEGLPTHLKRTILLVFSITLHNIPEGLAVGVAFGAAATADNPTAAILAAVS
                     VALGIGIQNFPEGAAVSIPLRQEGLSRKKAFVYGQASGIVEPIAGVIGALLVTKVELL
                     LPYALAFAAGAMIYVVVEELIPEAQQTLSSKRHFAVFGVMSGFIIMMILDVALG"
     gene            complement(6862..7059)
                     /locus_tag="ACG8KR_002424"
     CDS             complement(6862..7059)
                     /locus_tag="ACG8KR_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0201746.1"
                     /translation="MRQKNMFLNIFMVLLVLIAASFVISLFVSVLSGLLWFAVKVLIP
                     IALIIWLVRWIKGNTHRKNYY"
     gene            7228..7806
                     /locus_tag="ACG8KR_002425"
     CDS             7228..7806
                     /locus_tag="ACG8KR_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HFQ0201747.1"
                     /translation="MPKNRLEAFTDAVIAIIMTILVLELHEPNGDTWEALAALGYRFF
                     IYIVSFIILAIYWNNHHHLFQVVHKINGRVLWANSFFVFALSLFPFATSWISEYINSL
                     APEMTYGFVTLTANIAYYILAKELVKANKGHEKALKAMLNNYKKSYLSIGLNVLGIVL
                     GYLVAPVAVLIANIVILAAWIIPSRTIESQYR"
BASE COUNT         2379 a         1458 c         1579 g         2567 t
ORIGIN      
        1 ccactaaacg gtgttctcct gccaactcct cggtcacaag tcacaatatt caacaaacat
       61 gagaagctgt tttttgaaat tgttccttgt gactcggagc taaacggcag gctcacaacc
      121 tcttggtaaa cggtgttcaa tcagctgacc ctcggtatta gctcaaaaaa cggcaaaata
      181 tgaggaacta ttttttccgt ttttttctct aatactcagg tcaaaacgct gatttcacaa
      241 cctctgattc acggtgttcc agaagtagct tctacggaaa taagccgaaa tttcacaaaa
      301 atttgagaag caattttcgt aaattccttc ttatttcttg aagctgacca cttctgtcac
      361 aacctcttat tttgattaga acttgcgaaa ataaaccggt taaagtgtgt ctagcgcaag
      421 agatttgcta taatagacga taagggtaaa atgtaacgag aaggggttga aaaatgtgta
      481 tcaaattgta acagattctt gttgtgatct gccataccat gtattagcaa aagaaaaagt
      541 tgatttttta tcaatgaatc tccaattgaa tgatcaagag ttagtagatg acctcggaaa
      601 gacattcaat tatgaccgct acatgaatca gctgaaaaat ggtgcgatgc ctacgacatc
      661 acaaatcaat gttggccgat atttagaatt tttccgttct tatgcggaaa aacagattcc
      721 tgttttatat gtggcttttt ccagtggatt aagcggctct tacagtagtg cgcttcaagc
      781 ggttgaattg ctgaaggaag aatatgaaga ccctgagatc tatgtttttg atacaaaagc
      841 tgcaagtatt ggggaagggc ttttagtatt ggaagctgta agattgaaaa aagaaggtaa
      901 aagcatcgaa gaaaccctag agtggctgga aagtaattat ttgaaagtcc attcatgggt
      961 aacagtagac gatctgaaat atctagaacg aggcggtagg atttccaaag caagtgctac
     1021 tcttggcagt ctattgagtg taaagcctat tattacggtg gataaagaag gaaggctgac
     1081 gaatgttgac aaagttcgag gaagaaacaa gtcgatccag acaatcgtca atgagacggt
     1141 aaaaacaatc gttgcaccac ttgatcaaat cgtttatgtg gcttacgctg gggatctaga
     1201 gagcgcggag aaggcaaaac ggcttttgga agagcagata aagatgaaag atgtgaagct
     1261 ttatccctta ggaccaacga tcgccagtca cacaggctat ggctgtatcg cgattttttc
     1321 gatgggaatc agtcgataaa agcagtaact cattctgtaa aaaagcccag aaattgattg
     1381 taacaaaaac agcgactctt aaaagataga tgagcatcct atctcttaga ggtcgctgtt
     1441 tttttatttt ttaagctaaa taggccatta tgcccataac tcgttcaatt gtttttgaat
     1501 atctgcattt tccaaaaatt catcatatgt cgtatcagct cgatcgatca cacctttatc
     1561 cgaaacggca atgatgcggt ttgccaatgt ttggataaat tgatggtcat gtgacgcaaa
     1621 taagattgag ccagtaaatg ccatcaagcc gtcattcaat gctgtgattg attctaagtc
     1681 taaatggttc gttggatcat ctaatacaag tacgttagct ttcgaaagca tcatttttga
     1741 aagcatgcaa cggactttct ctccaccaga taaaacattc actggtttca atacttcttc
     1801 accagaaaac aacattcttc ctaagaagct tcttaagaag gtgttatcat cttcttcttt
     1861 acttgcatat tgacgcaacc agtctaaaat cgttagatca gtatcgaatt ctttcgttgt
     1921 atcctttggt aaataagaat tacttgttgt cacaccccaa cgtactgttc cagtatctgg
     1981 cgtaatttca cccatgataa ctttgaatag cgttgttgtt gttatatcat tttctgcgac
     2041 gaaagcgacc ttgtcttctt tgctcaaatt aaaagatata tcatctaaaa ttttctttcc
     2101 atcaatcgtt acacttacat tttctacctg caataagtcg tttccaatct cacgttcagg
     2161 tttgaatcca acgaatggat aacgtctaga agaaggttgg atatcgtcta aagtaatttt
     2221 ttcaagcatt tttttacgtg aagttgcttg ttttgatttt gatgcattcg cactaaagcg
     2281 ggcaataaat tcttggagtt ctttgatttg ttcttctttt ttggcatttg cgtttgcttg
     2341 aagtttagcc gctagttgac tagattccaa ccaaaaatca taatttccta cataaagttt
     2401 gattttacca aaatctagat ccgccatatg tgtacatact ttgttcaaaa agtgacggtc
     2461 atgggataca acgatcacag tattttcaaa attgatcaaa aattcttcca gccaattgat
     2521 cgattggata tccagaccgt ttgttggttc gtctagcaaa agaacatcag gtttaccaaa
     2581 tagtgtttgt gctaataaca cttttacctt ttgtccacca gttaattcac tcatttgctg
     2641 atggtgcaat tcttcaggga tgttcagacc ttgcagtaaa acagctgctt ctggttcagc
     2701 ttcccatcca ttaagctcag caaattctcc ttctaattca gctgctcgaa taccatcttc
     2761 gtcagtgaaa tcctctttca tgtaaatcgc atctttttct ttcagcactt catacagacg
     2821 cttgtgtccc atgatgactg tttcaagtac agtatagtct tcatagtcgt aatggttctg
     2881 tttcaatacg gccatccgtt catctggtcc catagtaacg gaacctgtgg aaggctgcag
     2941 atcccctgat aacactttaa ggaacgttga tttacctgca ccatttgcac caatcaagcc
     3001 atagcagttt ccgggggtaa atttaatatt cacatcatca aatagtttgc ggtctgaaaa
     3061 gtgcaaactc acatcattta cagtaatcaa tttttttcct cacttttatc attattagtt
     3121 ttgacaagtc ttatagaatt atatagggga aaggtaaaag attcaaggca atcgaacatt
     3181 ctttgtgttt cacgtgaaac agaatgaaaa aaataaaaat aaaagcggag cgaaaatagc
     3241 tactatagga tttattagtt tgcaacagca taatttttga cgttatttat aagagggagt
     3301 actataaaac tgtaaaagaa atgaaagaag gattgaaaat ggatgaaaaa agaaccatcg
     3361 actggggctc attgattttg ggaattttat ttgtacttgt ctcattgtta tcatttcaag
     3421 accctgtcgg aaatttagtt gcaattgtag tagtctttgc gatttttgcc tttattaaag
     3481 ggatatttga actgtttgta cggaacagat taaaagagct aacaggatat aaaggaaaga
     3541 tgcccttaat aatcgggatc atcgatattc tagttggcgt ctttttctta ttcaatattg
     3601 gtgcaggagt cgcagcattg ccttttgttt ttgcagtatg gtttattgct gattcagttt
     3661 tagcgttatt tactgctgac ttggcaagag gagtaagtga aggatattat tggtttacga
     3721 tcatcgtcaa tattttagga attttattag gaattttctt attgtttaat ccaatttctt
     3781 ctgcgttgac attaagcttt ttagttggat tctattttat gttatttggt atcacacata
     3841 tcgtctatgc atttagataa gtaaaatcaa acaaataaaa aagccggctt ccgattacag
     3901 ttttactgtt gtaattggaa gccggctttt ttatcatgga caaatatgca cgaggtgatt
     3961 tttaggtgtc gagacaaatt tatgcaaaaa tgtgagaagc tatatacaca aattttatct
     4021 taatactcga agcatagcgc agttgtcaca acctcttttc atataattca ttcaatactg
     4081 gcaaatcatg gacttcatat ttttctttga atgcttggat ttcttcttca gaatattttc
     4141 ttggatctaa atgcagatct tctactggca atgggcgctt gttattgatc ttcacatcga
     4201 ttactacagg acgatctgat tttgcagctg catcaaaggc aaatcgaagt tgcgagtatt
     4261 ctgtgactgt aaagctattt gccccgagcg cttctccaac tttaccgaaa tctgcatctt
     4321 ctaaaaatac accaaatttc ttttgttccg tatcttcttg ttctgcttcg atgaaaccaa
     4381 aagaatcatt cgaaaagaca acattaataa ttggaagctg atatttgact tgtgtaatga
     4441 tatcttgcat gaccatagca aacccgccat caccacttaa ggtaaatact tgtttttctg
     4501 ggtagcttag ttgtgcggca attcctcctg gcacaccatt acccatagtt gcgaaccatc
     4561 cagatgtcgt atgtttttgt ttaccgttca tattcaataa acgaatagaa tggatagtcg
     4621 tgttaccgac atctgtgacg aaaatcgcat cgtcttcagc aatacgattg atttctttat
     4681 agacagcctc tggacgaatc ggttcttcct ctcgatcttc aaaagatttc aaccaattta
     4741 cccatttttc tttattttct tgatttgcta ggtaccagct atcagcaggg cgagcatccc
     4801 ctaactcgac aagtctttgc aaagctgtgt ttgcatcacc gaggatcgac aaagatgttt
     4861 catggcgtcg tccaaatttt gacgcatcga tatcgatttg gataaattca gcagttggat
     4921 taaaaaatgc acgtccaaat gggaagtcgc taccgacaaa taagatcaga tcggcttctg
     4981 ccaatgcttc atttccgggt ttagtagcga cacgtccagc aaaaccaaga aagttttcat
     5041 aatcatcagg aataattcct tttgctagaa cggaagcgac aacaggaact gaaaaatgtt
     5101 ctgagaattc tttgatttgc ggaaatccgt ttcgcgttcc ttgaccaaca taaagaactg
     5161 gtttttttgc tttttcaaga taaggcaaag cagctagcaa atctttttct tctggtaata
     5221 tcacacttgt tttgtgagtg tgagcggtgg agaaaggttg ttcgtcaatt tcttcaaaac
     5281 caagatcaac agggatagtc acgatagcca cgcctttatg ctcataggcg gctttaatgg
     5341 cttcatctac tacatgcgga agactttgtg gtgtcatgac tgtacgattg tatacgctaa
     5401 catctgcgaa aataggattt tcatttagtt cctggaaaga gttatagttc atggatgtag
     5461 aagcaacttg tcctaagaac gctactagag gcacatggtc catctgtgca tcatacaaac
     5521 cattgatcaa atgtgtcgca ccaggtcctg cagagccgaa gacagcgccg actttccccg
     5581 ttaattttgc atctgcagcg gctgcgagtg cgccgacttc ttcatgacga acttgaatgt
     5641 agttgatttc ggttttttct tgatatagcg catccataat tgaattgaaa gatcctccgg
     5701 gaatgccgta aatatggtcg ataccccagc tttcaagaac ttttaccatt gcgattcctg
     5761 cattgatttt tgacatggtg tttcactcct ttagcaatga tgatacataa attttttgta
     5821 tctagagtat agaacttttt atcgaaaatc ccaactaatt cgtcttttca caaaaatgta
     5881 gtcatgtttt tgggaatatg ctagagtagt acatagatag attcaagaga ggggaatggc
     5941 atggttgaat ggctattgag acttgaacct tggcagcaag cacttgctgg gacagctttt
     6001 acttatttta tgacagctct tggtgccggg ttggtttttt tctttaaaga aatcaaaaaa
     6061 gaagtgctaa acttgatgtt aggatttgct tctggtgtca tgattgctgc aagtttttgg
     6121 tctttgttag atccggctat tacaaaagca gaagaaaatg gagatattgc ttggctagtt
     6181 gtaagtattg ggtttggttt aggcggtgtg tttttatata tggcagataa aacactgccc
     6241 catatgcatt ttggaccaca acatgaagca gaaggattgc ctacccattt aaaacggacg
     6301 atcttgcttg ttttttctat tacattgcat aacattcctg aaggtttagc cgtgggggta
     6361 gcattcggtg cagcagcaac tgcggataat ccgacagccg ctatattagc cgctgtttcg
     6421 gtggcgctag gtattggtat ccaaaacttc ccagaaggag cagctgtatc aatcccgttg
     6481 cgacaagaag gtctgagtcg taaaaaggcc tttgtttacg gacaagcttc tggaatcgtt
     6541 gaaccaattg ctggagtgat tggagcgcta ctagtaacga aagtggagct gctgcttcct
     6601 tatgcacttg cttttgccgc aggagcaatg atttatgtcg tggtggaaga attgatccct
     6661 gaagcacagc agacattgtc cagcaaacgt cattttgcag tttttggcgt catgagtgga
     6721 tttatcatta tgatgatttt ggatgtggca ttaggataaa taaaaaaaat atatatgata
     6781 tatcaaatag ggccgcgaac ttatatcgcg accctatttt tttagtaagt tatatcataa
     6841 tgaagtgaat ggtatcatac attaataata gttttttcta tgtgtatttc ctttgatcca
     6901 gcgaacaagc cagatgataa gtgcgattgg gattagaact ttgaccgcaa accataaaag
     6961 cccgcttagg acactgacaa aaagactgat gacgaagcta gctgcaatta gcactaaaag
     7021 aaccatgaag atatttaaga acatattttt ttgacgcata caaaaccctc ctcttgttca
     7081 ttctttacta tagaaaaagt ataacaggga ttgtttaaaa acacctccaa ctgaagactg
     7141 atttttactt catctactta aatgatcaaa caattgcttt ttctctggtt tatcatctaa
     7201 aatagagcgg aaaaggagga gaatatcatg cctaaaaatc gtcttgaagc atttacagat
     7261 gctgtaatcg cgattatcat gactatttta gttttagaac ttcatgaacc taacggagac
     7321 acgtgggagg cgctcgctgc attaggttac cgatttttta tctatattgt cagttttatc
     7381 attttagcga tttattggaa caatcatcat catttatttc aagttgtaca taagataaat
     7441 ggacgggtct tatgggcgaa cagtttcttt gtttttgcgc tttcgctttt tccgtttgca
     7501 acttcatgga tcagtgagta catcaatagc ctggctccag aaatgactta tggttttgtt
     7561 accttgaccg ctaatattgc gtattatatc ttggcaaaag aacttgtcaa agcaaataaa
     7621 ggccacgaga aagcattgaa ggcgatgttg aataattaca agaaatcgta tctatcgatt
     7681 ggtttgaatg tgttaggaat tgtcttaggg tatttagtag cgccagtagc cgtgctgatc
     7741 gcaaacatcg tgattctagc tgcttggatc atcccaagtc gtaccatcga atcacaatat
     7801 cggtaaaatg atccgtgttt aaaatttaag agaaaaatat agaaaaaata aataaacgat
     7861 caaatatccg agtgatacta gtattcaaag agggatgcag atggatcaaa aaagaattca
     7921 tgtaggactg attactagtt ttgtcacgat gcttatttat ttggatcaag gtcatttgac
     7981 ttg
//
LOCUS       DAZKLD010000132         7426 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.133, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000132 DAZKLD010000000
VERSION     DAZKLD010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7426)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7426)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7426
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.133"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(51..962)
                     /locus_tag="ACG8KR_002426"
     CDS             complement(51..962)
                     /locus_tag="ACG8KR_002426"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFQ0201748.1"
                     /translation="MKKRFLALAIVLGTGLLSGCTNAGEKTAVSYKGGTISEQEVMDS
                     LKKMQGADSAVQQLIVYQVFEDKYGDDVSTKEIDSQYDQTKKQLGDSFDSQLKSAGYT
                     EQTFKDSIKQSLAFQEGLKKHIKLTDEDLKTAWESFHPEVEAQIIQVASEDDAKDVKK
                     AADKGDDFSKLAKDKSTDTTTKEDGGKVKFDSTTTTVPAEVKEAAFKLKDGQVSDVIT
                     STNASTYTTEYYVVKMVKNQNKGNDMDKYKKELKEIATDTKLSDSTFQNKVIGEVLKD
                     ANVKIKDKDFENVLSTFTSDSSTASSK"
     gene            complement(1339..1977)
                     /locus_tag="ACG8KR_002427"
     CDS             complement(1339..1977)
                     /locus_tag="ACG8KR_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0201749.1"
                     /translation="MEPLIVIKGAKKIIRNKTILHLDRFTADAGDFVTVVGESGAGKS
                     TLLNILGLNDQFSSGQFYLFNQETKHLTGREKRLIKRNALSFLFQDFGLIEYETIDFN
                     LDIGLKFSNLNVSDKRTAKYEALKKVNINKKLSTPIYTLSGGEKQRIACARVLLKNSK
                     IILADEPTGSLDKRNKEQVVEILSQQASQGTLVIVVTHDPYLTKIGNKKIML"
     gene            complement(1981..3963)
                     /locus_tag="ACG8KR_002428"
                     /pseudo
     CDS             complement(1981..3963)
                     /locus_tag="ACG8KR_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370994.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
     gene            4143..4808
                     /locus_tag="ACG8KR_002429"
     CDS             4143..4808
                     /locus_tag="ACG8KR_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0201750.1"
                     /translation="MNIGEKIRTYRRNCKLTQKELGEKIGVSDKTISSWENSRTMPDL
                     EMLSLLHQTLGLPIDFPSIESATESIEPTLPDEPLVIKKSYSSKQPFSLKKTLSFFLL
                     VFCLVYLFLINYQYKNETVDRFNPFLKPQIGYTTLPVGYVENSIEHWVCNDIFVHGTW
                     LSFHAGEVEKGKNHAVIKHKGKHVMNVRLLTWEAIPSDYQEILIQDYDDALKRSPQEP
                     DRH"
     gene            complement(5205..7238)
                     /gene="brxL"
                     /locus_tag="ACG8KR_002430"
     CDS             complement(5205..7238)
                     /gene="brxL"
                     /locus_tag="ACG8KR_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease Lon-related BREX system protein BrxL"
                     /protein_id="HFQ0201751.1"
                     /translation="MEQQEILKLSEFFPGKIVRKDLTKKIKEGANVPVYVLEYLLGMY
                     ASSLDETLVEKGVENVKNILARNYVRPDEAEKVKAKIRELGGYTVIDKVTVHLNEKKD
                     CYEAEFSNLGLKNVQISEEYPKKFDRLLGGGIWCIVSMEYWYDENDRWNSPFIAKKVD
                     PIQMPGLDIEAYKMARKEFTTNEWIDLLLRSIGMEPTQFNKRVKWLHLARLIPLIENN
                     FNLCELGPRGTGKSHVYKEISPNSILVSGGQTTVANLFYNMASRTMGLVGMWDCVAFD
                     EVAGIKFKDNDGVQIMKDYMASGSFARGKEEKNASASMAFVGNINQSVDVILKTSNLF
                     EPFPEVMGTDSAFLDRMHCYLPGWEIPKYRPDFFTNDYGFITDYFSEVMRELRKVSFG
                     DSFDKYFKLGSQLNQRDTIAVRRIVSGMMKLLYPDGRFEKSDVEEILRFALEMRRRVK
                     EQLKKIGGMEFYDVNFSYIDLEEEREEYVSVPEQGGGTLIPEGLGKPGHLYTVSYGKT
                     GQLGTYKLETQMTSGNGKFERTGINSDAGAREAMDSAYRYLRANYGSVSQSISLTTKD
                     FVVHVQDMNGIGMTKTLSLSTIIALCSVSLNKPVLPSLVILGDITIGGSMIKVDNLAN
                     CLQVAKDAGAKKVLVPMISAADIGTVPPELMGTFNLIFYQTPEDAVFKALGVE"
BASE COUNT         2382 a         1439 c         1098 g         2507 t
ORIGIN      
        1 gatgaacagt tttattgatt taaatagttc atatttttta tttataccgc ttatttacta
       61 cttgctgtac tagaatctga agtgaatgta ctcaaaacat tttcaaagtc tttgtctttg
      121 atttttacat tagcgtcttt caatacttcg ccaattactt tattctggaa ggtactgtca
      181 cttaattttg tgtctgttgc aatttctttc aattcttttt tgtatttatc catgtcgttg
      241 cctttgtttt ggtttttcac cattttaacc acataatatt ccgtagtata agttgaagca
      301 ttggtagaag tgatcacatc agaaacttga ccatctttca acttaaaggc tgcttctttg
      361 acttctgccg gaaccgttgt agtagtggaa tcaaatttca cttttccgcc atcttctttt
      421 gtcgtagtat cagttgactt gtctttggct aatttagaga aatcgtctcc cttatcagca
      481 gctttcttca catcttttgc atcatcttca cttgctactt ggataatttg agcttccact
      541 tctggatgga aagattccca agccgttttt aaatcttcat cggtcaattt gatatgtttc
      601 tttagacctt cttgaaaggc tagactttgt ttgatactgt ctttgaatgt ttgttctgtg
      661 taacctgcag atttcaattg tgaatcaaat gaatcaccca attgtttctt tgtttgatca
      721 tattgggaat cgatttcttt ggttgatacg tcatctccat atttatcttc aaatacttga
      781 taaacgatca actgttgcac agcggaatct gctccttgca tttttttcaa gctatccatt
      841 acttcttgtt cgctgattgt accgccttta taactaacgg ctgttttttc accggcattc
      901 gtacagcctg acaataagcc agtgcctagt acgatggcaa gtgctaaaaa tctttttttc
      961 atttatcaat tctcctactt ttcgtttttt gcgaattgct aaaaacaaag gtgttcaaga
     1021 tgaaaatcat caagaacacc tttactaatc tgctcgatac gtcctgaata gatataaggc
     1081 tacacaatcc agaaagttcg ctcagcagaa taaatgtcaa gtagcacatc acaccttaat
     1141 atactacttc ttttgttacc gtttttcaag tctatcttgc gttccgtatt taacgtaact
     1201 aattcttaga acaaatgtta tcgattttgt ttttcttctt ccgctgttgg gatattcaaa
     1261 tcagacactt ttatgttcga ttttccagac atttttttgt tgtttttatt agaaaaagtt
     1321 tgttttttca tttgttttct aaagcatgat cttcttgttt cctattttgg tcagatatgg
     1381 atcatgcgtg accacgataa ctaacgtacc ttgggaagcc tgttgcgata agatttcaac
     1441 gacttgctcc ttatttcgtt tatctaatga tccagttggt tcatcggcta aaatgatttt
     1501 gctattcttc aataagactc tcgcacaggc gattctttgc ttctcaccac cagataatgt
     1561 gtagataggc gtacttagct tcttgtttat atttactttt ttcaatgcct catactttgc
     1621 tgttcgctta tctgaaacat ttaaattcga aaacttcaat ccaatatcta aattaaaatc
     1681 gattgtttca tattcaatca acccaaaatc ttgaaaaaga aaagagaggg catttctttt
     1741 tatcaagcgc ttctctcttc cagttaaatg ttttgtttct tgattaaata aataaaattg
     1801 ccctgaactg aattgatcat tcaatcctaa tatattcaat aaagtacttt tccctgctcc
     1861 gctctctcca actacagtca caaaatcccc agcatccgca gtgaaacgat ccaaatgaag
     1921 aatcgttttg tttcgaatga tttttttcgc accttttatc actatcaacg gctccaattg
     1981 ttaacctcca ttcatcagca ataactgact tttcttttct gtagacaaaa taaaaacaac
     2041 tgaaagaata aaatcaatga agctaaagaa aaaaatatta aaaagatatt taggctctct
     2101 ttctataaga aaaaggaaga ttacacttgt cgttccccat ttgatgaggt tcatcatgaa
     2161 gaaatcttta tacgtttcaa agagcgaata accacttgaa cgaaagatag cgattttccg
     2221 acaattgtag acaaaatata tgcataacat gacatagctc aacaagaaga aagaaaagaa
     2281 agtgagtaaa aatatctgta tcaagtaatg aaatgatctg cttaacactt tgacagctat
     2341 ctcttcagtc tgagtatacg gaaatgataa ctgcaagtta tcacgtaact gactttcttc
     2401 aatcagctca aataaccttt ctttcgacaa agagcctatc ttgatcttta acggtggata
     2461 gatttctccg ctgagaatat ttctttccca atacgtactg tttttcaatg tcagtatttc
     2521 aacaaccgga taatgttcga tccaggggat agaaggaata aatgtataaa tgggttgatt
     2581 gttttttata aatagaaatt tcggttcttt ttcactgatt tcttgataat attctgttat
     2641 ttgcgccagg tcatttttta aatctgtatg tctatatcgt tcagggatca atataacagg
     2701 gtgcgtttct ttttcggtta tttggatcaa ccttccagag acatcaatca aaggaaattt
     2761 cttaagataa ttcagattta tttttatccc tctgccaaac tgttcatttt cttcattcaa
     2821 gtaatctgat acattgacta atatacttcc gtttttattc aaagtttcat ataattcctg
     2881 atcggctgct gatgcaaaac gattggaata aataaaatcc acgtggtttt tcccgatggt
     2941 tactggataa aatacatgat agtcctcacc agcgatttct tttggtgttt catgggaaac
     3001 agctaactgt gaagaagaaa aaacgatagt tgctaattcg acattggcaa cggtcaaaag
     3061 aagagaaaac aacaatccaa atgtaaaaag aatgtattgg atatagttga acgcttctct
     3121 taacgaagcg attttttcaa ctaataaaag agaaatcaaa gagataccct atattccgct
     3181 gaacaagaaa acgtacatct ggaacaaata aagaaattgc tccttataat acataaaaat
     3241 aaaaccacca atgattcctg caattatgaa tgtaataatg attttctttt tccccaataa
     3301 acaccacaat acttgatgta atgaaaagcc attattcaac aataaactga tttctttatt
     3361 catcaataag aaaaaggatg ataacaaaag gaataaaaga atcaatccaa tagttaaata
     3421 cactcgataa ttatatacgt ctgtacttga aataaccgaa tacgaatagc tattttcaaa
     3481 gtcttcaaaa atattagtgg cataatcagt gtgaaatgct tgattgaatc gttccacaaa
     3541 tagtaaaatg aaccgttcat aggcttcttt ttctctcgtc atcaagaaaa attcaccttc
     3601 aaaatctttt attttgatcg attcttcaat ggacgttacg cgaaaggagg tattagaata
     3661 gggtaccttc tcgtgagaag atttgacatc cccttgataa tacaatgtca tttcaagctt
     3721 tggctgtatc ttgaagttcc aagatgtatc tacttcattg gattcattta ctgttttttt
     3781 catataagaa agctcagttt ctgatgagac attttcaaat accatgatta gctgctcata
     3841 ttcttcttgc ccaataaaat gttgtggcaa tcgaatcgga gtataagtat ctgtttgatg
     3901 gaaaaattca tttgtccgat agagtacctg tttatttata ataaccaagg caaaaccact
     3961 catgataccg gtcactatga ttgtgagtaa aactgtgaac ttttttttga ttatcaccac
     4021 tctcccttac ttaaatgatt ctttgataaa atcataccgt ttttcatcaa aaaaacgtat
     4081 ctatcaaatg tataggtcat gtagaataaa tctatatact gtagaatcga aaggagaaga
     4141 agatgaacat cggagaaaaa atacgaactt accgcagaaa ttgtaagctt acgcaaaaag
     4201 aattaggcga aaaaatcggt gtttccgata aaacaatctc tagttgggaa aattctcgta
     4261 caatgccaga ccttgaaatg ctgtcacttt tacatcagac tttaggtctg ccgattgatt
     4321 ttccttctat tgaatcagct accgaatcaa tagaaccaac attgccggat gaacctcttg
     4381 ttatcaagaa atcttattca tcaaaacaac ccttctcact caaaaagaca ctatctttct
     4441 ttctgctcgt cttttgtttg gtctatcttt ttcttatcaa ttatcaatat aaaaatgaaa
     4501 cggttgatcg attcaatcct tttttgaaac cacaaatcgg ctataccaca ctccctgtag
     4561 gatacgtgga aaattccatc gaacattggg tatgtaatga tatctttgtt catggtacat
     4621 ggctgtcttt tcacgctgga gaggtagaaa aaggtaagaa tcatgctgta atcaaacaca
     4681 aaggaaaaca cgtcatgaac gttcgcctgc tcacttggga agccattccc tcagattacc
     4741 aagaaatcct catccaagac tatgacgatg ccttaaaaag aagccctcag gagccagata
     4801 ggcattaagt aggagatgaa cgtagagata gaaaaaatca atgcacatga tcttatcaaa
     4861 aaaaaacagg ggtacaaatt aattgaaaaa gctttgagat gaaagttcct aaataaaaaa
     4921 gttcagaatg ataggtaaaa aaatgactaa ctattctagt attaggctat ttgtattatt
     4981 cttctgttat ttttatatca aaaaatccag aaataactaa atataggtat ttctggctta
     5041 tagtataata tttacgccac agcccccaat gattcttttt ggaatcatga tcaaggacgg
     5101 acatacacaa aaatattttg cccattcgat ccgaatagat taacgactaa tctagtaagt
     5161 aatatagatt ggtacgtagt caaaccagca aatggaaaag ttgtctattc caccccaagg
     5221 gctttaaaga cagcatcttc cggcgtttga taaaaaatca aattaaaggt acccattaat
     5281 tctgggggta cagttccaat atcagcagcg cttatcatag gtacaagtac ttttttcgct
     5341 ccggcatctt ttgctacttg taaacagtta gctaaattat ctaccttaat catagaacca
     5401 ccaattgtaa tatcccctaa aataactaaa cttggtaata caggtttatt taaggaaact
     5461 gagcataaag ctattatagt tgataacgat aatgtttttg tcataccaat gccattcata
     5521 tcttgaacat gaacgacaaa atcttttgtc gtcaaactaa tagactgact aacacttcca
     5581 taattagctc ttaaatatcg gtatgccgaa tccatcgctt ctcttgctcc agcatctgaa
     5641 ttgatcccag tacgttcaaa ctttccattt cccgaagtca tttgcgtttc caatttatag
     5701 gtacctaatt gcccagtttt gccataagat accgtgtaca aatgccctgg cttccctaac
     5761 ccttctggaa tcaaagttcc tccaccttgt tcaggaacag aaacatactc ttctcgctct
     5821 tcttctaaat caatgtatga aaaattgaca tcataaaatt ccattccacc aattttcttt
     5881 aattgttctt ttacacgacg acgcatttca agagcaaatc ttagtatttc ttcgacatca
     5941 cttttttcaa atctaccatc aggatacaac agtttcatca tcccagatac aatccgacga
     6001 actgcaatcg tatcccgctg atttaattga gaaccaagtt tgaaatattt atcaaaacta
     6061 tcgccaaaac taacttttcg aagctcacgc atgacttcag aaaaataatc tgtaatgaag
     6121 ccatagtcat ttgtaaaaaa atctggtcga tatttaggaa tttcccaacc tggtaaataa
     6181 caatgcatcc gatccaaaaa ggctgaatcc gtccccataa cctccggaaa tggctcaaat
     6241 aaattcgaag ttttcaatat tacatcaacg ctttgattaa tatttcctac aaaagccatt
     6301 gatgctgaag cattcttttc ttctttgcct ctagcaaaag atcctgaagc catgtaatct
     6361 ttcatgattt gaacgccatc attatcctta aatttaatcc cagcaacttc gtcaaaagca
     6421 acacagtccc acatacctac aagtcccatt gtacgactag ccatattata gaaaaggttt
     6481 gctactgttg tttgtccacc tgaaacaagg attgagtttg gtgaaatttc tttatataca
     6541 tgcgatttac ctgtaccacg tggtcctagt tcacagagat taaaattatt ttcaattaat
     6601 ggaataagtc ttgctaaatg aagccatttt acccttttat taaattgtgt aggttccata
     6661 ccaatggacc gtagaagtaa gtcaatccat tcatttgtag taaactcctt gcgagccatc
     6721 ttgtaagcct caatgtctaa accaggcatt tggattggat caaccttttt tgcaataaaa
     6781 gggctattcc aacgatcatt ctcatcatac caatattcca tcgaaacaat acaccagatc
     6841 cccccaccta ataatcgatc aaattttttc ggatactctt cactaatttg aacatttttt
     6901 aatcccaggt tagagaattc tgcttcataa caatctttct tttcatttaa atgtacagtt
     6961 actttatcaa taactgtata accacccaat tcacgaattt ttgcttttac tttttctgct
     7021 tcatcaggac gcacatagtt tcgagcaaga atatttttaa cattttccac acctttttca
     7081 accaatgttt catccaatga cgaagcatac ataccaagta aatattccaa tacataaaca
     7141 ggtacattcg caccttcttt aattttcttt gttaaatctt ttctgacaat ctttccggga
     7201 aagaattcag ataactttaa aatttcctgt tgttccacta gaattcctcc atgtgttcag
     7261 tcattataat agcaattaaa gtatatcata cttaaccagt aggcttgata atacaaaaga
     7321 aaaagtatcc acaaaaaatt gtggatacct cgtttttata tatcaaaatc aaatgattct
     7381 gcaaaaggat tatctataat gaattttatt cgctcggtca aatcat
//
LOCUS       DAZKLD010000133         7316 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.134, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000133 DAZKLD010000000
VERSION     DAZKLD010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7316)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7316)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7316
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.134"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..386
                     /locus_tag="ACG8KR_002431"
     CDS             <1..386
                     /locus_tag="ACG8KR_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0201752.1"
                     /translation="DRLTCSNCGTLFYYEEVRNIVKHRKKEMPIRCPNCNEVIKRKLS
                     HGYFVSYELYEESKINLKKKPKYYKNLKTGAIITEKEFKNLVTEEESRFYNLSGNICP
                     GFMDQIIENESIESIENYVPVDLPK"
     gene            complement(559..762)
                     /locus_tag="ACG8KR_002432"
                     /pseudo
     CDS             complement(559..762)
                     /locus_tag="ACG8KR_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
     gene            728..925
                     /locus_tag="ACG8KR_002433"
     CDS             728..925
                     /locus_tag="ACG8KR_002433"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0201753.1"
                     /translation="MKDSIVEPEGMENTFELYNKTTVDKLIPILVPTDLNGHDVLLRF
                     NGFLGEDYRYLKISDPQPLTE"
     gene            complement(968..1543)
                     /locus_tag="ACG8KR_002434"
     CDS             complement(968..1543)
                     /locus_tag="ACG8KR_002434"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HFQ0201754.1"
                     /translation="MAQLFYRYGTMNSGKSIELLKVAHNYEEQGKRVIIMTSAIDTRS
                     GFGKVESRIGVSKPAIAITSEMDVYGELVEIIDQYGKVDCILVDESQFLSRENIIDLS
                     LIVMNEEVPVICYGLKNDFQNNLFKGSEALLTFADKIEEIKTICHFCNKKAVMNARIV
                     DGIPVYSGEQIVVGGNEDYLSVCRNHYYNED"
     gene            complement(1698..2224)
                     /locus_tag="ACG8KR_002435"
                     /pseudo
     CDS             complement(1698..2224)
                     /locus_tag="ACG8KR_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            2681..4192
                     /gene="gadC"
                     /locus_tag="ACG8KR_002436"
     CDS             2681..4192
                     /gene="gadC"
                     /locus_tag="ACG8KR_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015082498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate:gamma-aminobutyrate antiporter"
                     /protein_id="HFQ0201755.1"
                     /translation="MNKKKLSLFGFFALTASMVLTVYEYPTFATSQLHLVFFLLLGGI
                     LWFLPVALCAAEMATVEGWKDGGIFSWVSQTLGERFGFAAIFFQWFQITVGFITMIYF
                     ILGALSYVINFPELNTDPFIKFIGVLVIFWVLTFSQLGGTNRTAKIAKAGFVIGIVIP
                     SIILFGLAAAYFIGGNPIQMAINAKAFIPDFSQLSTLVVFVSFILAYMGVEASASHIN
                     ELENPQRNYPLAMFLLVILAIALDAIGGFSVAAVIPQHDLSLSAGVIQTFETLILHFN
                     SHLHWLVKVIALMIACGVMGEVSSWVVGPSRGMFAAAQRGLLPVFFRKTNKQDVPVPL
                     IMIQGIIVTIWAAVLTFGGGGNNLSFLVAISLTVVIYLVGYLLFFLGYFVLVFKKKDL
                     KRTYNVPGKTVGKTIIGGIGFILSVFALLISFVPPASIAASEHRTYQIILLISFVVTV
                     LLPFIIYEVHDKSAHTTIEEPTHVKAEDVNPAVYPAARGEHHVTKNDEHILKH"
     gene            4205..5605
                     /locus_tag="ACG8KR_002437"
     CDS             4205..5605
                     /locus_tag="ACG8KR_002437"
                     /EC_number="4.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004351 - glutamate decarboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006540 - glutamate decarboxylation to
                     succinate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HFQ0201756.1"
                     /translation="MLYGKDNQEEKNYLEPIFGSASEDVDLPKYKLNKESIEPRIAYQ
                     LVQDEMLDEGNARLNLATFCQTYMEPEAVKLMTQTLEKNAIDKSEYPRTTEIENRCVN
                     MIADLWHAPNNEKFMGTSTIGSSEACMLGGMAMKFAWRKRAEKLGLDIQAKKPNLVIS
                     SGYQVCWEKFCVYWDVELREVPMDEKHMSINLDTVMDYVDEYTIGIVGIMGITYTGRY
                     DDIKGLNDLVEAHNKQTDYKVYIHVDAASGGFYAPFTEPDLVWDFQLKNVISINSSGH
                     KYGLVYPGVGWVLWRDQQYLPEELVFKVSYLGGEMPTMAINFSHSAAQLIGQYYNFVR
                     YGFDGYRDIHQRTHDVAVYLAKEIEKTGIFEIINDGSELPVVCYKLKEDPNREWTLYD
                     LSDRLLMKGWQVPAYPLPKDLDQLIIQRLVVRADFGMNMAGDYVQDMNQAIEELNKAH
                     IVYHKKQDVKTYGFTH"
     gene            complement(5770..7205)
                     /locus_tag="ACG8KR_002438"
                     /pseudo
     CDS             complement(5770..7205)
                     /locus_tag="ACG8KR_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328084.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
BASE COUNT         2313 a         1222 c         1306 g         2475 t
ORIGIN      
        1 atgatagatt gacgtgttct aattgtggta cattatttta ctatgaggaa gttagaaata
       61 ttgtaaaaca tagaaaaaaa gaaatgccta tacgttgtcc aaattgcaat gaagttatta
      121 agcgaaagtt gtctcatggt tattttgtca gctacgaact atatgaagaa agtaaaataa
      181 atctgaaaaa aaaaccaaaa tactataaaa atttgaaaac tggagctatt ataactgaaa
      241 aagaatttaa aaatttagtg actgaagaag aaagcaggtt ttataatttg tctggtaata
      301 tttgccctgg atttatggat cagattattg aaaatgagtc aattgaatca attgaaaact
      361 atgttcctgt tgacttacca aaatagaaaa aataatcctg tttttacagt gaattatttt
      421 aattttgatt ttcaataaaa atatattaca ataataatcg taatagctaa tgaaacaatt
      481 tctcaatctt tttaccactc caaacaggga gcgtcaataa ttttgtgtaa ataactcgtc
      541 cttctgcaaa ataatgggtt actcagtaaa cattgaagct aatgtatccg tcacttgttg
      601 gaagccttta tggcttctat ttaagaattt ttgattgtat gtatcaaaga tggatactag
      661 gaaacgctct agtgattctt cattttgaaa ctgctctttt ctgcggctgt atttcttgat
      721 ttgtttattg aaagattcga ttgtagaacc agaaggaatg gagaacactt ttgaacttta
      781 caacaaaact actgttgata aattaattcc aattttagtt ccaactgatt taaatggtca
      841 tgatgtttta cttcgtttta acggattttt aggtgaggat tatagatacc taaaaatttc
      901 tgatcctcaa cctttaacag aataataaaa aacgtgtaat caattatttg attacacgtt
      961 ttttatttca atcttcgttg tagtagtggt tgcgacatac cgaaaggtaa tcttcattac
     1021 ctcctactac aatctgttca cctgaataaa ctggaatacc atcaacaata cgagcattca
     1081 ttacagcctt tttattgcaa aaatggcaaa tagttttaat ttcctcaatt ttatcagcaa
     1141 atgtcaataa tgcttcggat cccttgaaga gattattttg aaaatcattc ttcaatccat
     1201 agcaaattac tggtacttcc tcattcatta cgataagtga tagatcaata atattctctc
     1261 tacttaaaaa ttgagactca tcgaccaaaa tacaatcaac ttttccgtac tgatcaataa
     1321 tctccactaa ttctccgtac acatccattt cagaagtaat tgcaatagct ggtttcgaaa
     1381 cacctatacg actctcaact tttccaaatc ctgatcgagt atctattgcg cttgtcatta
     1441 tgataactct cttcccttgt tcttcataat tatgagccac tttcagtagc tcaatactct
     1501 ttccactatt cattgttcca tatctgtaaa ataattgtgc cattaaattt attcccttct
     1561 tttacaattc aattattcct taattccatc tgcttttatg cagctgctcg tttattcctt
     1621 gatatgtcta taacatctac tatccctaaa accaatttat aagccattag agatggtatt
     1681 cctgaacatc tctcatattg ttttttgagt ggtatatcat ctgaaaaact gaaggaatca
     1741 tgaaaatagc gacatactta tcatttagag ttctaacttt aaatgcattt acttcttcag
     1801 tcagcacttt agtcatgttg gacatggttt ggggcgtgta gtgatgccca tacattttct
     1861 caatcaagtc tgcgatttcc gacatggtaa taccttttcg gaagaggtga atgacggtct
     1921 cctctaatgt gtcattcgtc cgtctataag caggaacagt ctgttgcttg aactcgccat
     1981 tgcggtcgcg cggaatctgg agatgaagtt ccccatactc ggtcttgacc gtacggtcat
     2041 aggagccgtt acgagaatta cccgtgttaa aaccaatgcg atcgtacttt tcgtaatcga
     2101 ggaaagccgt taactccgtt ttgagaagcg tattgacagc tttttccaaa tggacacgga
     2161 ataatccatt caaatcgcct ttgttcgcta gagtctttag gatttctgta gtaaaatcgt
     2221 tcatatggaa gtcctctttt ctgtgaatgt gttgtggtaa ctctattcta cagaagggac
     2281 ttcctttttt ctatttacac aaaatatttt acactctcaa caaaagtatc ttcgattaat
     2341 acttgctcaa gttatgtgca gctacgaaaa agtaggtacc gaggtacttt ctttttcgca
     2401 gctttttttg gacttatgag aagtacattc gctggaagaa tacgatataa aacgagcttt
     2461 ttcactaaag atattaatag agtgaaagaa acaagctttt gttgtcagtt ttttcacttt
     2521 tctaaaaatg aaaataaaaa attctggaag tggagaagaa ttttgcttgc tatactttgt
     2581 cttgtagaaa agaggtggac aaaataatgc aagctcaaat aagcgatttt ccataacctt
     2641 ggtaggaatg acaaattaaa taggaaaggg aagtaataat atgaataaaa aaaagttatc
     2701 tctattcggt ttttttgccc tgacagcttc catggttttg actgtttatg agtatccgac
     2761 ctttgcaaca tcacaattac atttggtttt ctttcttctt ttgggaggta ttttatggtt
     2821 tctaccagtt gctttatgtg ctgcagaaat ggcaacagtt gaaggatgga aagatggtgg
     2881 gattttcagt tgggttagtc aaactttagg agaacgtttt ggttttgcag ctattttttt
     2941 ccaatggttt caaattacag taggttttat tacaatgatc tacttcatac taggagcgct
     3001 gtcgtatgta attaattttc cagagcttaa tacagatcca tttatcaagt ttattggtgt
     3061 attagtcatt ttctgggtac tgactttttc acagttgggc gggaccaatc gtaccgctaa
     3121 aattgctaaa gcaggatttg tcattggaat tgttattcct tcgatcattc tctttggttt
     3181 agcagcagcc tactttatcg gcggtaatcc gattcaaatg gctattaatg cgaaagcttt
     3241 tattcctgac ttttctcagc tttcaacttt agtagtattt gtatctttta tattggctta
     3301 tatgggcgta gaagcttcag catcacatat taatgagctg gaaaatcccc aaagaaacta
     3361 cccattggca atgtttttac tagtgatttt ggccatcgcc ttagatgcaa tcggcggttt
     3421 ctcagtagca gcggttattc cacaacatga cttgtctctc agtgctggtg taatccaaac
     3481 atttgaaacg cttattttac attttaacag tcatttacat tggttggtaa aagtgattgc
     3541 tctcatgatt gcctgtggcg ttatgggtga agttagttct tgggtagttg gtccatcacg
     3601 agggatgttt gcagctgctc agcgaggtct gttaccagtt ttcttccgga aaactaataa
     3661 acaagacgtt ccagttccgt tgattatgat acaaggaatt atcgttacta tttgggcagc
     3721 agtcttaact tttggtggag gaggcaacaa tttatcattc ttagtagcaa tctccttaac
     3781 tgttgtcatt tatctcgtag gatatttact atttttccta ggttactttg tattagtttt
     3841 taagaaaaaa gacttaaaac gaacttacaa tgtaccagga aaaactgttg gcaaaacgat
     3901 tatcggtgga attggtttta ttttatcagt gttcgcattg cttatctctt ttgtaccacc
     3961 agcttcaatt gcagctagtg agcaccgaac ataccaaatc atactactta ttagctttgt
     4021 agtgactgtt cttttacctt ttatcattta tgaagtacat gataaaagtg ctcacacaac
     4081 tattgaggaa cctactcacg tgaaagctga agacgttaat cccgctgttt atccagctgc
     4141 tagaggagag caccacgtta caaaaaatga tgaacatatt ttaaaacatt aggaggaatt
     4201 cattatgtta tacggaaaag ataatcaaga agaaaaaaac tatttggaac caatttttgg
     4261 ctctgcaagt gaggatgttg acttgcctaa atataagtta aacaaagaat ccattgaacc
     4321 acgaattgct tatcaattag tacaagacga gatgttggat gaaggaaatg cgcgattaaa
     4381 cttagctact ttttgtcaaa cgtatatgga acctgaagca gtgaaattga tgacccaaac
     4441 gttagaaaaa aatgcaattg ataaatcaga atacccacga acaacggaaa ttgaaaaccg
     4501 ctgtgtaaat atgattgctg atttatggca tgctccaaat aatgaaaaat tcatgggaac
     4561 ttcaacgatc ggctcttcag aagcctgcat gctgggtggt atggccatga aatttgcttg
     4621 gcgtaaacgt gctgaaaaat taggtcttga tattcaagca aaaaaaccta acctggtgat
     4681 ctcttctggt taccaagttt gttgggaaaa attctgtgta tattgggatg tggaactgag
     4741 agaagtccca atggatgaaa aacatatgtc aattaatcta gatactgtca tggattatgt
     4801 ggatgagtac acaattggta ttgtaggtat tatgggtatt acttacactg gtcgttatga
     4861 tgatatcaag ggtctgaatg atttagttga agctcacaat aaacaaactg actataaagt
     4921 atacattcat gttgacgctg catcgggtgg cttttatgca ccatttactg aacctgatct
     4981 agtttgggat tttcaattga aaaatgttat ctcaattaat tcttcaggtc acaaatatgg
     5041 tttggtatat ccaggtgtgg gttgggtctt atggcgtgac caacaatact taccagaaga
     5101 attagtattt aaagttagtt acttaggtgg agaaatgcca actatggcta tcaacttctc
     5161 tcatagtgca gcacaactaa ttgggcaata ctacaacttt gtacgctatg gctttgatgg
     5221 ttatcgtgat attcaccaaa gaactcatga tgttgctgtt tatttagcca aagagatcga
     5281 aaaaactggt atttttgaaa tcattaatga tggatcagaa ttgccagttg tgtgctataa
     5341 gctgaaagaa gatcccaatc gcgaatggac actatatgat ttatctgatc gtctgttaat
     5401 gaagggatgg caagtcccag cctacccatt acctaaagac ttggatcaat taattattca
     5461 acgcttagtt gttcgagcag actttggaat gaacatggct ggtgattatg tacaagatat
     5521 gaaccaagca attgaagagt tgaataaagc tcatattgtt tatcataaaa aacaggatgt
     5581 gaaaacgtac ggctttactc actaaaattc ttcagagaaa tataaaagaa gttaattaac
     5641 taataaaagg agttgtgatt accacacagc tccttaaatc tatttgaata aatatatgat
     5701 attcatttgt tacactctca atcttagctg ttgttctatt catcattact cttattgtaa
     5761 gtagttcagt caaatttgct tgttcaattg atcgataaca gtatgaattg tttgaaaagt
     5821 aatcggatat ataattgttt tctcactacc taagccttct tggtataaag aactatcgca
     5881 aagtataatc cgctgagtac aaaagaaagt gtcgtctact atttctctaa gttgtttaca
     5941 aatgatatta ctaatagaaa agtgttctcc caaaatgtct tctaactgtt gtcgatagta
     6001 taaataccta aagtctgacg gagctactat gaacacttca attggagatt tccgtttatg
     6061 ctcatttaaa agacttaaac taatggttag tttacgaata gaattctcat tccaacgcag
     6121 aaataaattg ttttgttttc gccattgttc aagaatttca tgaacttcta agtacaaagc
     6181 gcgttgctct tcatttaaca agtaagtctt atcaggttga aatatctgaa tatcagccca
     6241 aagtgagcgg ataaacggga gaagtgcttt atcaaaaagt atctgatttt cttcagatat
     6301 attcagagat aagataagtt tttgactgag ctcaatcacc aaagggttat ctgctacatg
     6361 gttttgataa acaacgttca agtctttttg taacaattca aagttatcgc atagataatt
     6421 tctagagttg aatagaaagt agatataaag gcattcatct tcgtcatgac ataaagagaa
     6481 agaaagttgc tctaatcctt tctgtattag ctgaaacaag ggagtatgtc gggcaactct
     6541 tttttcttcc tgactaaaat atattgggtt agtcttagat ctctgcaaac cgatagcgat
     6601 acctaataaa ataaattgtt tttcaactgt agggtaactt cttgaaaaaa atgtttttct
     6661 acataatcaa tcaattgttg accgctttga ataattgatt tatttagctg gatcaactct
     6721 gtccgatttg tctcatagat tatcgttagt aacaaacttc gataatcctt ttcaggaatt
     6781 tcaaaatggc catcagcatt taagtactgc atttgtttaa aaaaattaat caactcattt
     6841 cgtaagttgt aaactttgct taacgaaatg aaggtagtat cagctatatt actccaattg
     6901 tgttcccctg ataaataatg tcctagtaca ttgaaaaagt cagagcgctg atagatgagt
     6961 tgagtgagtg ccaacctcga aaacgaagag tcgaatgcga ctgtaaagct acttctagat
     7021 ttctccactg aaataatttg ctgatggagt atgtcggtaa tgcattccaa gtcgttagtg
     7081 acagtgagtg ggcaaacgtt caattgatct gctatttcgc gaatctgaat ggtccctttt
     7141 tcgtgtaatt gctcacaaat gtaaacttga cgaaaaatac ttttctccag ataggtttcc
     7201 aacatagtac tcctcctagt cattttatac ataactatta taactctacc aatcggaata
     7261 gtcctgtaat atattgaatc aaaaatataa attataaaat aattagatgg ggtatt
//
LOCUS       DAZKLD010000134         7275 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.135, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000134 DAZKLD010000000
VERSION     DAZKLD010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7275)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7275
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.135"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            123..770
                     /locus_tag="ACG8KR_002439"
     CDS             123..770
                     /locus_tag="ACG8KR_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0051775 - response to redox state [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HFQ0201757.1"
                     /translation="MREKTDTLPKATAKRLPLYLRYLKMLDDSGISRIKSNEFSEITQ
                     IPSATIRRDFSQLGELGRSGYGYDVPFLIDVFNNILNTKEEKRIALVGYGNLGKALKH
                     NNFRRNENLNIVCVFDNDPALINRVIDGEMIYPIDRFAEIAKAKNVTVAISTVPSKYS
                     QSAIDEIVKGNVTAILNFAPDRVTVPAYVNVQYIDLTTELQTLIYFDENYSEVFS"
     misc_binding    899..1115
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1163..3808
                     /locus_tag="ACG8KR_002440"
     CDS             1163..3808
                     /locus_tag="ACG8KR_002440"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HFQ0201758.1"
                     /translation="MTEENNLSTKYNPQEVEAGRYQHWLEQDLFKPSGDKKAKPYSIV
                     IPPPNVTGKLHLGHAWDTTLQDMLIRQKRMQGYDTLWLPGMDHAGIATQAKVEAKLRE
                     QGISRYDLGREKFIDQVWEWKEEYASHIREQWAKMGLSLDYSRERFTLDDGLSEAVRK
                     VFVTLYEKGLIYRGEYIINWDPQARTALSDIEVIHKDIEGAFYHMSYELADGSGVVEI
                     ATTRPETMLGDTAIAVHPEDERYQALIGKKVILPLVNKQIPIIADEYVDMEFGTGVVK
                     ITPAHDPNDFEVGNRHDLPRVNVMDDDGTMNELAGKYAGMERFAARKAIVQDLKEIGR
                     LIKIDTMIHSVGHSERTGVVVEPRLSTQWFVKMAPLAKKAIDNQETDQAVEFFPPRFN
                     QTFLRWMENVHDWVISRQLWWGHQIPAWYHKETGEMYVGMEAPADSENWVQDPDVLDT
                     WFSSALWPFSTMGWPDTESEDYKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGERPF
                     ENVLIHGLIRDEQGRKMSKSLGNGIDPMEVIEKYGADALRWFLSNGSAPGQDVRFSYE
                     KMDASWNFINKIWNASRFVIMNIEGMTVEEIDLSGEKSVADRWILTRLNETIERVTEL
                     FDRFEFGEAGRQLYNFIWDDFCDWYIEMSKEVLYGDDAVSKQTTRSILVHTLDQILRL
                     LHPIMPFVTEEIWEHIPHQGNSLVVAEYPVVRPEFDDETASKGMEVLKELIRAVRNIR
                     SEVNTPLSKPITLLIKTNDAKIDQFLVENTSYIERFCNPEELTISSEITAPDLAMSAV
                     LTGAEIFLPLAGLINIEEEIKRLEKELEKWTSEVKRVQGKLSNERFVSNAPEEVVEAE
                     RAKEKDYLEKQTAVKERIESLRTIQ"
     gene            4118..5431
                     /locus_tag="ACG8KR_002441"
     CDS             4118..5431
                     /locus_tag="ACG8KR_002441"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HFQ0201759.1"
                     /translation="MTIEEAIEWIHSRLPFGSRPGLDRVEALLEVVGHPERKVKTIHI
                     AGTNGKGSTVAYLRSLLEEAGLTVGTFTSPYIETFNERIAINGKSIPDNDLIQLVKKY
                     QPLIKQLDQFEAVAGITEFETLTAMAFDYFLMKKVDVAIIEVGLGGLLDSTNVAVPML
                     TGITTIGLDHTEILGDTIEEIAAQKAGIIKQGVPVVTGNIAPEAFTVIQQTAKAKNAP
                     LYVYGENYKINYLHPDEMWGEVFEFFNEIGKIPQLTTPLLGQHQAENAGVAIELYFVY
                     CQLEKLPFREKDVLKGLKKSRWPGRMERLSNEPLVVLDGAHNTHAMNRLVENLKKEFK
                     GYQINILFSALRTKNVREMLERLQQVPNVHIYLTTFEHPKAIELSEVSDLASERVSIV
                     SLWQFGLADILEKMTNEDVLVVTGSLYFISEVRHLLIQLGGDHEV"
     gene            5421..6074
                     /locus_tag="ACG8KR_002442"
     CDS             5421..6074
                     /locus_tag="ACG8KR_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFQ0201760.1"
                     /translation="MKYNAVIFDMDGLLFDTELVYYEATQVVADKMGFPYDKDLYLKF
                     LGVSDEEVWANYHMIFSEFGKTSVQRFIDDSYQETLERFSQGAVQMKPGVLELLSFLE
                     EHQIPKVVASSNQRAVIELLLKKNNLFERFETIVSAENVQRAKPDPEIFLLANQKLGT
                     AKKETLILEDSQNGILAASAAEIPVVMVPDLLPPSQELIKKTAGVVSSLHEIPALLK"
     gene            6129..6821
                     /gene="radC"
                     /locus_tag="ACG8KR_002443"
     CDS             6129..6821
                     /gene="radC"
                     /locus_tag="ACG8KR_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HFQ0201761.1"
                     /translation="MGKRLIKEVPTSSLPRERMEIYGAEALSDQELLAILLRTGQHPY
                     SVMSIAGNLLKTFGGLASLRQATLHELEEIRGIGRVKAIEIKALIELGRRIQTDHERA
                     APPVRTSYELAQQLILEMKDHKQEHLVCIYLDTKNQVILKKTVFIGSLNQSVAHPREI
                     FHYAVRYCAARIVLAHNHPSGNVIPSQQDMNFTKRIQKCGEMMGITVLDHLIIGRKRY
                     FSLREEGMMEEK"
     gene            6971..7171
                     /locus_tag="ACG8KR_002444"
     CDS             6971..7171
                     /locus_tag="ACG8KR_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFQ0201762.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
BASE COUNT         2402 a         1234 c         1597 g         2042 t
ORIGIN      
        1 atgcaagctt ttgaacaccg ttttatttgt ctcacaccct tttccttcct acttgtaacc
       61 ttgctcccgt gttagaaaca tgttacacta aatatgtgaa attattaaca aggagcgtcg
      121 ttatgcgaga aaaaacagat acactgccaa aagcaactgc gaaaagatta cctttatatt
      181 tacgctactt gaagatgctg gatgactcag gtataagtag aatcaaatca aatgagttca
      241 gcgaaatcac ccaaattcct tctgcaacga tccgcagaga cttctcgcaa ttaggcgagc
      301 taggcagaag cggatatggc tatgatgtcc cttttcttat cgatgtattc aataatatcc
      361 ttaatactaa agaagaaaaa cgaatcgcat tagtaggata tggaaatctt ggcaaagcat
      421 tgaaacacaa caattttaga agaaatgaaa atctaaatat cgtgtgcgtc tttgataatg
      481 atccagcact gatcaatcga gtaattgatg gagaaatgat ctatccgatt gatcgtttcg
      541 cggagatagc caaagcaaaa aatgtgacag tagcaatctc tacggtacca agtaaatatt
      601 cacaatctgc tattgatgaa attgtcaaag gaaatgtaac cgctatactt aattttgcgc
      661 ctgacagagt aactgtccca gcttatgtca atgttcaata cattgatttg acaaccgagt
      721 tgcagacgct tatttacttt gatgagaatt actcagaagt tttttcttag gaaaatttga
      781 gtaggcttat tgatcttgcg agaactcgtc gtaattcttt caaaaatttt ttagtactaa
      841 atgaagtggt tttttcacga ccacttgttg actaaaaaaa aaaaccattg tagaatgaag
      901 cataacgctg aacaagagga gtaactggca cattcattct tagagagaga actatctgct
      961 gaaaggttct caatgacaac cagtgaaggt agcttgggag tttctgtctt gaatggagta
     1021 gagatagacg agtcgtgatc gttaactcac ttgagaggca ataatgattg attattgcaa
     1081 tttaggtggt accgcgtaaa tcatacgtcc tagaatacga aagtatttta ggacgttttt
     1141 tagtttaaag gaggaaaaca gcatgacgga agaaaataac ctatcaacaa aatataatcc
     1201 gcaagaagtt gaagctggac gttatcagca ttggttagaa caagatttgt ttaaaccgag
     1261 cggagataaa aaagcaaaac cttattcaat cgtaatcccg cccccaaatg ttactggaaa
     1321 acttcattta gggcatgctt gggatacgac cttgcaagat atgctgattc gtcaaaagag
     1381 aatgcaagga tatgatactt tatggctgcc tggtatggac catgcaggaa tcgcgacaca
     1441 agcaaaagta gaagcgaagc ttagagagca agggatttct cgttatgatc taggtcgtga
     1501 aaagttcatc gatcaagtgt gggaatggaa agaagagtat gcgagccaca tccgcgaaca
     1561 gtgggctaaa atgggcttat ctttagatta cagtagggaa cgttttacat tagatgatgg
     1621 attatctgaa gcagtacgta aagtttttgt taccttatac gaaaaaggtt tgatttatcg
     1681 tggtgaatat atcatcaact gggatcctca agcacgaaca gctctatctg atatcgaagt
     1741 tattcataaa gatatcgaag gcgcctttta tcatatgagc tatgaactag cagatggatc
     1801 aggcgtagta gaaattgcga caacccgtcc agaaacgatg ttgggagata cggcgattgc
     1861 tgttcatcca gaagacgagc gttatcaagc gctcatcggc aaaaaagtga ttctaccttt
     1921 agtcaataaa caaatcccaa tcattgcaga tgaatatgta gatatggaat tcgggacagg
     1981 tgtagtaaaa atcactccag cacatgatcc aaacgacttt gaggtaggta atcgtcatga
     2041 tcttccacgc gtaaatgtta tggatgatga tggtacgatg aatgaactag caggaaaata
     2101 tgctggaatg gaacgttttg ctgctcgtaa agcaatcgtg caagatctga aagaaatcgg
     2161 tcgattgatt aaaatcgata cgatgatcca cagtgtgggt cattccgagc ggacaggtgt
     2221 cgttgttgaa cctcggctgt ctactcaatg gtttgtaaaa atggcgccgt tagcgaaaaa
     2281 agccattgat aaccaagaaa cagatcaagc agtggaattc ttccctccaa gattcaatca
     2341 aacgttttta cgttggatgg aaaatgtcca tgattgggta atctcacgac aattatggtg
     2401 gggacatcaa attcctgcat ggtatcacaa agaaacaggt gaaatgtatg tagggatgga
     2461 agcaccagcg gatagtgaaa actgggtaca agatcctgat gttttagaca cttggtttag
     2521 ttcagctttg tggcctttct caacaatggg ctggccagat acggaaagcg aagattataa
     2581 gcgatacttc cctactagca ctttagtaac cggttatgac atcattttct tctgggtaag
     2641 cagaatgatt ttccaaagct tggaatttac tggcgaacgt ccatttgaga atgtcttgat
     2701 tcatggattg attcgtgacg aacaagggcg gaagatgagt aaatcattag gtaatggaat
     2761 cgatccgatg gaagtaatcg aaaagtatgg agcagatgct ctccgctggt tcttatcgaa
     2821 tggttctgca ccaggacaag atgtccgctt cagctatgaa aaaatggatg cttcttggaa
     2881 ctttatcaac aaaatctgga atgcttcacg tttcgttatc atgaatatcg aaggaatgac
     2941 cgtagaagag atcgatttat ctggagaaaa atcagtggct gaccggtgga tccttactcg
     3001 tttgaatgaa acgatcgaac gagtaactga gctatttgat cgttttgaat ttggtgaagc
     3061 aggtcgacaa ctgtataact ttatctggga tgatttttgt gactggtaca tcgaaatgag
     3121 taaagaagta ctttatggag atgatgctgt ttcaaaacaa accacccgta gcatcttagt
     3181 ccatacactt gatcagatcc tacgtttatt gcatccaatc atgccattcg ttacagaaga
     3241 aatatgggaa catattcctc atcagggaaa ttctctagta gtggcagaat atcctgttgt
     3301 acgtccagag tttgatgatg aaacagcttc taaagggatg gaagtattga aagaattgat
     3361 tcgagccgtt cgaaatattc gttcagaagt gaatacacca ttgtctaaac caattacgtt
     3421 gctaattaaa acaaacgatg ctaaaatcga tcaattttta gttgaaaata caagctatat
     3481 cgaacgcttc tgtaatcccg aagagttgac gatatcaagt gagatcactg cgccagatct
     3541 agcgatgtca gctgttctga caggtgcaga aatcttcttg ccgttagcag gattgatcaa
     3601 tattgaagaa gaaatcaaac gattagaaaa agaactggaa aagtggacaa gcgaagtaaa
     3661 acgtgtccaa ggaaaacttt ctaatgaacg ttttgtttct aacgcgccgg aagaagtagt
     3721 agaggcagag cgtgcaaaag aaaaagatta tttagagaag caaacggctg tcaaagaacg
     3781 aatcgaaagt ttgcgtacta ttcaataaga atagaaaaag agagtgtgaa atcagctagc
     3841 tattactaaa agctagctga ttgaacatcg ttacaggaaa gagtttgagc ctcactctta
     3901 gcttcgattc ataaggagat gtgacataaa ttttgtcgca tctctttttt agtctactct
     3961 tattatgtag gagaaggagg gaggtggatc atgtatcaag agtcaataaa gtagttgata
     4021 aaatcgttcc tctctctgtt tcttttctct ttgtcatact tttatttttc ttatgtgttc
     4081 tatttgttat aataaaagag aaatggggga attgttgatg acgattgaag aagcaataga
     4141 atggatccat agtcgattac catttggttc acgtcctggc cttgaccgag tcgaagcttt
     4201 attagaagtg gtagggcatc cagagagaaa agtaaaaacg atccatatcg ctggaacaaa
     4261 cggaaaaggt tctactgtag cttatttgag aagcctatta gaagaggctg ggctgacagt
     4321 aggtacattt acttcacctt atatcgaaac gttcaatgaa cggatcgcaa tcaatggcaa
     4381 aagcattcca gacaatgatt tgatccagct tgtaaaaaaa tatcagccac tgatcaaaca
     4441 acttgatcaa tttgaagcag tagctggtat tactgaattc gagacattaa cagcaatggc
     4501 atttgattat tttttgatga agaaagtgga cgtagcgatt atcgaagttg gcttaggcgg
     4561 cttattggat tctaccaatg tagctgtacc aatgctgacg ggaataacaa cgattggact
     4621 tgatcatacc gaaatacttg gagatacaat tgaagaaatc gctgcacaaa aagctggaat
     4681 catcaaacaa ggcgttccag tagtaacagg aaatattgcc ccagaagctt ttactgtcat
     4741 ccaacaaaca gctaaagcaa aaaatgcacc gctttatgta tacggggaaa attataaaat
     4801 aaactatctg catcctgatg agatgtgggg agaagtattt gaatttttta atgaaatagg
     4861 aaagatccct caattaacca ctccgctttt agggcaacat caagctgaaa atgcaggagt
     4921 agccatcgaa ttgtattttg tttactgtca attggaaaag ctgcctttcc gagaaaaaga
     4981 tgttttgaag ggattgaaga aatctcgatg gccaggaaga atggagcgtt tgagtaacga
     5041 gccattagtt gttttagatg gtgcccataa tacgcatgca atgaaccggc ttgttgagaa
     5101 tttgaaaaaa gaattcaaag gatatcaaat caacattttg ttttccgcct tacgaactaa
     5161 aaatgttaga gaaatgctag aacgattgca gcaagtacca aatgtccata tttatttgac
     5221 gacctttgaa catccaaaag ccatcgaatt aagtgaagta tcagatttag caagtgagag
     5281 agtatcaatc gtttcattgt ggcaattcgg attagcagat atattggaaa agatgacaaa
     5341 tgaagacgta ttagttgtga ccggttcact ttatttcatt tcagaagttc gccatttatt
     5401 aatacaatta ggaggagatc atgaagtata atgcagtgat ttttgacatg gatggtttgc
     5461 tgttcgacac agagttagtg tattacgaag ccacacaagt agtagcagac aagatgggtt
     5521 ttccttatga taaagacctg tatttaaaat tcttaggtgt ttcagatgaa gaagtatggg
     5581 cgaattatca tatgattttc tcggaatttg gcaaaacaag tgtacaaaga ttcatagatg
     5641 attcatacca ggaaacgctt gaacgttttt cgcaaggagc ggtacaaatg aagcccggtg
     5701 tgttggaact cctctctttt ttagaagaac atcagattcc caaagttgtc gcttccagta
     5761 accaacgagc agtcattgaa cttttattga agaagaacaa tctgtttgaa cgatttgaga
     5821 cgattgtttc tgcagaaaat gttcaacgag caaaaccaga cccagaaatt ttcttgttag
     5881 ctaatcagaa gctaggtaca gcgaagaaag aaacattgat tttagaagat tcacaaaatg
     5941 gtattttagc agcttctgcg gctgagatcc cggtagtcat ggtaccagat ctgcttccac
     6001 catcccaaga attgatcaaa aaaacagctg gggttgtttc ttctttacat gaaatccctg
     6061 cattgttaaa ataaaaatcc atctcttttc gtatcttatt ttagataaga tagaaaggag
     6121 atgttttttt gggaaaacga ttgatcaaag aagtacctac tagctcttta ccaagggaac
     6181 gtatggaaat ttatggcgcg gaagctttat cggatcagga gcttttggca atcttactgc
     6241 gtaccggtca acacccttat agtgtgatgt cgattgctgg aaatctgtta aaaacgtttg
     6301 gaggactggc ctctttacga caggctactt tgcatgagtt agaagagatt cgagggatag
     6361 gacgcgtgaa agcaatcgaa atcaaagcct tgatcgaatt aggtcgacga attcagacgg
     6421 atcatgaaag agcggctcca ccggttcgta ccagctacga attggcccag caattgattc
     6481 tggaaatgaa agatcacaaa caagaacatt tggtttgtat ttatttggac acgaagaatc
     6541 aagtgatctt aaaaaaaaca gtgttcatcg gttcgctgaa tcagtcagtc gctcatccta
     6601 gagaaatttt ccattatgct gtacgttatt gtgcagcaag gatcgtttta gcgcataatc
     6661 atccttctgg taatgttatt ccatcgcagc aggatatgaa ttttaccaaa aggatacaaa
     6721 agtgtggaga aatgatgggg atcactgtac tggaccactt gatcatcgga cgcaaacgat
     6781 attttagttt gagagaagaa ggaatgatgg aagagaagta agttgctttc ttgcaaaaaa
     6841 aatgaagcaa gtgtacaatt atattgtaat tttgtttggg aatagaagtt gaaacatctg
     6901 ttgcacctct atcgtacacg agcaatgtta caatttaaat tagtgctttt ggcacgagga
     6961 ggatattatt atggaaaacg gaacagtaaa atggttcaac gctgaaaaag gatttggatt
     7021 tatctcacgt gaagatggaa gcgacgtatt cgttcacttc tcagctatcc aaggtgacgg
     7081 attcaaaaca ttagaagaag gtcaagcagt gactttcgac gttgaagaat cagaccgcgg
     7141 cccacaagct gcaaacgttg taaaagctta attttatctg taaatcaaat tgatggtaga
     7201 catcaatttt tacgagagaa caaagcgagt aacaaaaata cctgccacaa gagacaattt
     7261 ctcttgtggc aggta
//
LOCUS       DAZKLD010000135         7157 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.136, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000135 DAZKLD010000000
VERSION     DAZKLD010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7157)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7157)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7157
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.136"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..402
                     /locus_tag="ACG8KR_002445"
     CDS             <1..402
                     /locus_tag="ACG8KR_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HFQ0201763.1"
                     /translation="SVENKKLDEILIESLIEKEWLYQVWGDNIVNCLKLVGNNDYSQF
                     YDWYKKFKYAIKSAEKEQKIQLKIIYEICNNKYFVDYVREQLSMTLRDLIRRAKTDHR
                     IKQKDDYIFASLKNKALELIELQISEGIDKQ"
     gene            478..696
                     /locus_tag="ACG8KR_002446"
     CDS             478..696
                     /locus_tag="ACG8KR_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0201764.1"
                     /translation="MIENRLIDQVWCLDQNTDNLELIETICFNTIVLDLRSENDNCVT
                     HIIIDRKTAQKLSESLRKWAEGGNNENS"
     gene            683..3376
                     /locus_tag="ACG8KR_002447"
     CDS             683..3376
                     /locus_tag="ACG8KR_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pLS20_p028 family conjugation system
                     transmembrane protein"
                     /protein_id="HFQ0201765.1"
                     /translation="MKIVKEKIASRKIHWLALSILVLMFIVVFTGNVVQASIFNWFDD
                     TESQTEFLNNPVYQSYLKKYNGNFYSQFGLWIGWSVIKSMFAIVDGIQNMIPEVLDLF
                     SFIESTGMNKVYQSVLSTVVIGLMILSLMFVGYKMITGKGTVDLKSVGMNVVMSVALI
                     LLMPTLIGSTEKLSGIMFAKTLYNDATSINNQDGGVAWSLIEQGVTDLAYINKTDQYS
                     TIDKTNDRNNLTKENFWKTDLTQVLTDNVIDKMEKENPTADNLRYELVENSDNEFVAT
                     KFSDNFLSTFVDGIKSGYYRYQANLWGISFGLIALAVAYVFSAFIIITAILELAFKRV
                     LGVLVFATDLETGQRSKQVLSDILQCYLTVGFQGFGLSMFAMFINYLNTGEGISANIF
                     IKTIAYICAVFVLIKGSGTVMRYFGVDIGLKEGYGQLASAFGMGAMLFRKGTGGMNRS
                     KGGKNNGGNDQAEGEERKPERNFGQSLSKKANKTGKALGYAHERGLSGLASDGSTMAS
                     ERATKPFKSMRDIANDTKSKFKEGLDDGTVSAINKNSKPMLAKNKEDETGKYADSMPT
                     RLSDRKDGAKVENADSIMSSSERMREAMKNNAESNNNPVSAIQQKVQQDIEERKNAMH
                     GQAKSAEELINQKRHEAKYTPEAMNREEIMLRKRVEGRTGANIDGKEALTKERIQEAK
                     NSNTGLEKLVKENPQNSTSSHGGRSVDVRENIQGSLNGHVGRNVDVRENLQSSSNAQG
                     TKTIDVRENLQSSSTSQPKTVDVRENVQNSTSSQGFKSVDVRENVQGSSASQPKTVDV
                     RENVQSSTSSQGFKTVDVRENVQKDSGEMKEKTQKINIVEDRKSAKKFDSNHETVIID
                     SEIRENDKGSKPRRRFTYEDNELFRDTLNDPNPLFDSLLKK"
     gene            3390..3587
                     /locus_tag="ACG8KR_002448"
     CDS             3390..3587
                     /locus_tag="ACG8KR_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0201766.1"
                     /translation="MQLILQISTHDFLELDELCESVKKATNEKITVTNYIELMIQEEL
                     KMKNEIESDFKCCSLEKNVRG"
     gene            3591..4202
                     /locus_tag="ACG8KR_002449"
     CDS             3591..4202
                     /locus_tag="ACG8KR_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="HFQ0201767.1"
                     /translation="MDDKQKMYEKNRKHSEALNFRVDKKTYLELTLIAKQRGISRADV
                     CRERLKGLKYEPPLLAEDDRVVLTKQLNQIGNNLNQAVKALNIIAKYFREESEDKKEK
                     SLHFSFDASRYNIFKDELANSIAKKLMISYPDDIFLESNRSAPSFFKLSEVEKEYFEY
                     WIEKQTRPGTKEEYSQKVDRNLIHSIQIIASLKDEVKQLWELL"
     gene            4187..5578
                     /locus_tag="ACG8KR_002450"
     CDS             4187..5578
                     /locus_tag="ACG8KR_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HFQ0201768.1"
                     /translation="MGATITSKGRKKNLSSLLQYAANPSKKEKCVAVSGINCSDDSEE
                     ALQEMNLVRTKMNKSKDIAQAFHVIHSFDKSTSKELSIDKMHEIAVEFAEKAFPNSQI
                     IVASHNDKDHFHSHLVINNINMETCKRLRIDPKDLELMRKINDQVLNEHGLESVNNEY
                     YENLEKRTNLYSNDPSKMKYGKINYQEFVQNAIEATLEKCEVNSFEDFSDHLAKEYNI
                     EVYQFSKTNNKLGYVLYKQGVNFYERRGELITLGRDKQSRKEKSQYVERTFSARKLGK
                     RYSFESVSEQLEKNLEESHIKNFFNMEREQVMSLISGVNYKTGELNVPFVELDSIAQK
                     QFSDREGDEKFIKYDDFLREVDKHFVNTMSLEDDQEYTIKLHTDVNPQTLRSAKSKTI
                     SIKKTDQKELFLPAKGFENCSHTPSAKQIHYQISRIMNDDVKMYRAKKDYEFTQQMIE
                     NQQKRQQNFEMEF"
     gene            5794..6108
                     /locus_tag="ACG8KR_002451"
     CDS             5794..6108
                     /locus_tag="ACG8KR_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0201769.1"
                     /translation="MNYPYFKVSASEETKEIFNNFYNQNKGVFGSKANMFRVMVSNLP
                     VLASPSNNKFNDSESIKFEQKISELESMISNEVIEKLDDIDQKLSYSLKNKYKTEEKK
                     DV"
     gene            6101..6595
                     /locus_tag="ACG8KR_002452"
     CDS             6101..6595
                     /locus_tag="ACG8KR_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0201770.1"
                     /translation="MFKVYFPFQWLVKGILLVLLGLILFGSYLTIKARHFPEEKPIKI
                     VTIDLKIGETYQADGVELTFKNIEVTEKRNEFAQHDRVLQVTYTLKNDTEKPIRPQQW
                     SIENKDGEELYEYAMILDNPEYIESDKEETVNYYYELPIERQDYQIVVRRANGYFVGL
                     PFTL"
     gene            6672..6986
                     /locus_tag="ACG8KR_002453"
     CDS             6672..6986
                     /locus_tag="ACG8KR_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0201771.1"
                     /translation="MAEENKVVPFETLVINKEEFEQLFLDFFIQYDRIYDFSSSLDLF
                     ELEAEIIENSREKNGKIIISVEKFNELFSETFEIDPDYYQPQEVFREFRNQIIRSSNI
                     EN"
BASE COUNT         2720 a          955 c         1398 g         2084 t
ORIGIN      
        1 tcagttgaaa ataaaaaatt agatgaaata ttaattgaat cattaattga aaaagaatgg
       61 ctttatcagg tatgggggga taatattgta aactgtctga aattagtggg taataacgat
      121 tacagtcaat tttatgactg gtataaaaaa ttcaaatatg cgattaaatc tgctgaaaaa
      181 gaacaaaaaa ttcaactgaa aattatctat gaaatctgta ataacaagta ttttgtagat
      241 tatgttagag aacagctatc aatgacttta cgtgacttga tacgaagagc aaaaacagat
      301 caccgtatca aacaaaaaga cgattatatc tttgcttctc taaaaaataa agcgttagaa
      361 ctaattgaat tgcaaatcag tgaaggaata gataagcaat aagaatagaa ttgagtcaaa
      421 tgaagtggag gggtatctcc gcttcatttg agaactttga atataggagg ggttattttg
      481 attgaaaatc gtttaataga tcaagtttgg tgtctagatc aaaatacaga taatctcgaa
      541 ttaatcgaga ctatttgctt caacactata gttctcgatt taagatctga aaatgacaat
      601 tgtgtaactc atatcataat tgacagaaaa acagctcaga agctctctga gagtctcaga
      661 aaatgggctg agggaggtaa taatgaaaat agttaaagaa aagattgcca gtagaaaaat
      721 acactggtta gctttgagca tacttgtttt aatgtttatc gttgttttca ccggtaatgt
      781 tgtccaagca agcattttca attggtttga tgacacggaa agtcaaactg aatttttgaa
      841 taatcctgtc tatcaatcgt atttaaaaaa atataacgga aatttttaca gtcagtttgg
      901 attgtggata gggtggtctg ttataaaatc gatgtttgca attgtagatg gtattcaaaa
      961 tatgattccc gaagtgttag atttgtttag ctttattgaa agtacgggaa tgaataaagt
     1021 ttatcaatcg gtcttaagta cagttgttat tggtttaatg atactttcac tgatgtttgt
     1081 tggttacaaa atgattactg gaaaaggaac agtggattta aaatcggttg gaatgaacgt
     1141 agtgatgtct gtagcattaa ttcttttaat gccgactttg attggatcaa cagaaaaact
     1201 ttcaggaata atgtttgcaa agactcttta taatgacgca actagtatca ataatcaaga
     1261 tggcggtgta gcatggtcat tgattgagca gggagtgaca gatttagctt atattaataa
     1321 gacagatcaa tattcgacaa ttgataaaac aaatgaccga aataatctaa caaaagaaaa
     1381 tttctggaaa acggatctta cgcaagtatt gacagataat gtaattgata aaatggaaaa
     1441 agagaaccca acagctgata atctgcgtta tgaattagtt gaaaattctg ataatgaatt
     1501 tgttgctact aaattttcag ataatttttt atcaaccttt gttgatggta tcaaatcagg
     1561 atattatcgc tatcaagcga acctgtgggg aatttctttt ggattgattg ctttagcggt
     1621 agcgtatgtt tttagtgctt tcattataat tacagcaata ctcgagttag catttaaacg
     1681 tgtactcgga gttttagtat ttgctactga cttagaaaca ggacaacgat caaaacaagt
     1741 tttgtcagat attttacagt gctatttgac agtcggattt caagggttcg gtttatctat
     1801 gtttgcaatg ttcataaatt acctaaatac tggcgaaggt atctcagcaa atatttttat
     1861 aaaaacaatt gcttatatct gtgctgtgtt tgttttaatc aaaggcagtg gaacagtcat
     1921 gagatacttt ggtgtggata ttggactcaa agaaggctat gggcaacttg cttctgcttt
     1981 tggaatgggt gcaatgctat tccgtaaagg aacaggtggt atgaaccggt ctaaaggtgg
     2041 gaaaaataat ggtggaaacg atcaagctga aggggaagaa cgaaaacctg aacgcaactt
     2101 tggacaatca ctttctaaaa aagctaataa aacggggaaa gcgcttgggt atgctcatga
     2161 acgtggatta tcaggtttag cttcagatgg atctacaatg gctagtgaaa gagcaacaaa
     2221 accatttaag agtatgcgtg atatagctaa tgataccaag agtaagttta aagaaggttt
     2281 agatgatggt actgtgtccg ctattaataa aaatagcaaa cctatgttag ctaaaaataa
     2341 agaagatgaa actggaaaat atgctgattc catgccaaca cgtctttcgg atagaaaaga
     2401 tggtgctaag gttgaaaatg ccgatagtat tatgtctagt tctgaacgta tgcgtgaagc
     2461 aatgaaaaat aatgctgaat cgaataataa tcctgtaagt gcaattcagc aaaaagtcca
     2521 acaagatatt gaagaacgta aaaatgctat gcacggtcaa gctaagtctg ctgaagaatt
     2581 aattaatcag aagcgtcatg aagcgaaata cacgcctgaa gcaatgaatc gcgaggaaat
     2641 aatgttaagg aaacgtgtag aagggcggac aggcgctaat attgacggaa aagaagcgtt
     2701 gaccaaagaa agaattcaag aagctaaaaa ttcaaataca ggattagaaa aattagtcaa
     2761 agaaaatcca caaaattcta caagtagtca tggtggccga tcagtagatg tccgagaaaa
     2821 tattcaaggt tcactgaatg gtcatgttgg acggaatgtt gatgtaagag agaatttaca
     2881 aagttcttca aatgctcaag gtactaagac aattgatgtt cgagaaaatc tgcaaagtag
     2941 cagtacaagt caaccaaaaa cagtagatgt tcgagaaaat gtacaaaatt ctacaagtag
     3001 tcaagggttt aaatcagtgg atgtccgaga gaatgtgcaa ggtagcagtg caagtcaacc
     3061 aaaaacagta gatgttcgag aaaatgtaca aagttctaca agtagtcaag gattcaaaac
     3121 agtagatgtt cgggagaacg ttcaaaaaga tagtggagaa atgaaagaaa aaacacaaaa
     3181 aatcaatatc gttgaagaca gaaagtcagc gaaaaaattt gattcaaatc atgaaacagt
     3241 gattattgat agtgaaatcc gtgaaaatga taagggatca aaaccaagac gaaggtttac
     3301 ctatgaagac aacgagttat tcagagatac acttaatgat cctaatccac ttttcgatag
     3361 tttacttaaa aaataaagag gtgattgtca tgcaattaat tctacagatt agtacacatg
     3421 atttcttaga acttgacgaa ttatgtgaat cagtaaagaa agcaacaaat gaaaaaatca
     3481 cggtcactaa ttatattgaa ctgatgattc aagaagaatt aaagatgaaa aatgaaatag
     3541 agagcgattt caaatgttgc tctctagaaa aaaatgtgag gggataatca atggatgata
     3601 aacaaaaaat gtacgaaaaa aatcgcaaac atagtgaggc cttaaatttt cgtgtcgata
     3661 aaaagactta tcttgaactg acattgattg caaaacaaag gggaatttct agagcagacg
     3721 tttgccgtga gcggttaaag ggactcaaat atgaaccgcc gttgttagct gaagatgata
     3781 gggtagtttt aaccaaacag ttaaatcaaa ttggtaacaa tttgaatcaa gcggtaaagg
     3841 cattaaatat cattgctaaa tattttagag aagaatctga agataaaaaa gaaaagagcc
     3901 tacattttag ttttgatgct agccgttaca acatttttaa agatgaactt gcgaatagca
     3961 tagcaaaaaa gttgatgatt agttatccag atgacatttt tttggagtcc aatcggagtg
     4021 ccccctcttt ttttaaatta tcagaagtag aaaaagaata tttcgaatat tggatagaaa
     4081 agcaaactag accgggtacg aaagaagagt attcccaaaa agttgatcgg aatttaattc
     4141 actcaattca gattattgca agtttaaaag atgaggtgaa acaactttgg gagctactat
     4201 aacatcgaaa ggacgcaaga aaaatttatc ctcgctatta caatatgcag ctaatccttc
     4261 aaaaaaagaa aaatgtgttg cagtctcagg aataaattgt agtgatgata gtgaggaagc
     4321 actgcaagaa atgaatttag tacgtaccaa gatgaataaa tctaaagata tagcacaagc
     4381 gtttcatgtt attcattcgt ttgataagag tacttctaaa gagttaagta ttgataaaat
     4441 gcacgaaata gctgtcgaat ttgcagagaa agcttttcca aattcgcaaa tcattgttgc
     4501 aagtcacaat gataaagatc attttcatag tcatcttgtc attaataata ttaacatgga
     4561 aacatgtaaa aggttgcgta ttgaccctaa agatttagaa ctaatgcgaa aaattaacga
     4621 tcaagtcctc aatgaacatg gtcttgaatc agtgaataat gagtactatg aaaacttaga
     4681 aaagagaacc aatctttatt caaacgatcc ttctaagatg aaatatggaa aaattaatta
     4741 tcaagagttt gtgcaaaatg cgatcgaagc aacgttggaa aaatgcgaag tgaattcttt
     4801 tgaagatttt tcggatcatt tggcaaaaga atataacatt gaagtttatc aattttcaaa
     4861 aacaaataac aaacttggct atgttcttta caaacaaggt gtaaatttct atgagcgtag
     4921 aggtgagtta attactttag gaagagataa acaatctaga aaagaaaaaa gtcagtatgt
     4981 cgaacgtact tttagtgcaa gaaagttagg gaaaagatat tcatttgaat ctgtttctga
     5041 acaacttgaa aagaatttag aagaatcaca tataaaaaat ttttttaata tggaaagaga
     5101 gcaagttatg tcgctaatta gtggagtaaa ttacaaaact ggtgagttaa atgtaccttt
     5161 tgtggaatta gattccatcg cacaaaagca atttagcgac cgtgaaggcg atgaaaaatt
     5221 cattaaatat gatgacttct tgcgagaggt agacaagcat tttgtaaaca caatgtcttt
     5281 agaagatgat caagaataca cgattaaact tcatacggat gtgaatcctc aaactttacg
     5341 tagtgcgaaa agtaaaacaa tttctattaa aaagactgat cagaaagagt tatttctacc
     5401 ggcaaagggt tttgaaaatt gttctcatac accttctgct aaacagattc actatcaaat
     5461 cagtcgcata atgaatgacg atgtaaaaat gtatcgtgct aaaaaggatt atgaattcac
     5521 acaacaaatg attgaaaatc agcaaaaaag gcaacaaaat tttgaaatgg aattctgaat
     5581 gggcttgtta gtggactttc cagttttttg cctatgaaga gttgcttgga aagaagcagt
     5641 ttttaggaca tacgccagct acccatctac ttgccagtca atccgccccg ctcacgctag
     5701 gctactttct ggcaagtcag atggtgggta aatctctgag ggagcaagct ccctacagac
     5761 tccctcccaa aaaaagaaag gtggaagaaa aaaatgaact atccatattt taaagtctcg
     5821 gcttcagaag aaactaaaga aatattcaac aatttttaca accaaaacaa aggagttttt
     5881 ggttccaagg caaatatgtt tcgtgttatg gtttcaaatt taccagtgtt agctagtcca
     5941 tcaaataata aatttaatga ttctgaaagc attaaatttg aacaaaaaat ttcagaacta
     6001 gaatcaatga taagtaatga agtaattgaa aagcttgatg acattgatca aaagttatcg
     6061 tattctctaa aaaacaaata caaaacggag gagaaaaaag atgtttaaag tttacttccc
     6121 gtttcagtgg ctcgttaaag gaattctttt agtgctacta ggattgattc tttttggaag
     6181 ttatctaaca attaaagcca gacattttcc agaagaaaag ccaataaaaa ttgtaacaat
     6241 agatttaaaa ataggtgaaa cttatcaggc agatggggtt gaactaacat ttaaaaatat
     6301 tgaagtgacc gaaaaaagaa atgaatttgc acaacatgat cgtgtattac aagtgaccta
     6361 tacacttaaa aatgatacag aaaaaccaat acgtccacaa caatggtcga ttgaaaataa
     6421 ggatggtgaa gagctttatg agtatgctat gatattagat aatccagagt atattgaaag
     6481 tgacaaagaa gaaacagtaa attactatta tgaattaccg attgaaaggc aggactacca
     6541 aatcgtagta cgtagagcga atggttattt tgttggatta ccctttactt tgtaagtctt
     6601 attttgttag tggcttttaa gttaatatca tttttgtgat ttttgaaaat gaaagtgagg
     6661 aaattggaag aatggctgaa gaaaataaag ttgttccgtt tgaaacatta gtgataaata
     6721 aggaagagtt tgagcaactt tttcttgatt tttttattca atatgacagg atttatgatt
     6781 ttagttcgtc actagattta tttgaattag aagcagaaat aatagaaaac agtcgagaga
     6841 agaatggtaa aatcattatt tcagtagaaa aattcaatga attattctca gaaacatttg
     6901 aaatagatcc agactattat caaccacaag aagtttttcg agagtttaga aatcagatta
     6961 ttcgttcaag taatattgaa aattgagtca ataaaagaag cgttgaagaa aagttccttc
     7021 tttcgacgct tctttttaaa agattttaca aatgtcttat ggagatttgc ttcaatgact
     7081 acttgtagtc atttcgttta aaataattat gttacaccct tatgtgtcac agagaaatac
     7141 tgatataata agaaata
//
LOCUS       DAZKLD010000136         7089 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.137, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000136 DAZKLD010000000
VERSION     DAZKLD010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7089)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7089)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7089
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.137"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1256)
                     /locus_tag="ACG8KR_002454"
     CDS             complement(<1..1256)
                     /locus_tag="ACG8KR_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086557923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFQ0201772.1"
                     /translation="MSHTQLIKDTLNILDLNIYFEENCLTKEKYKGQICLIYKGTLLY
                     KPEECSHCLCVVPSRIIRWGTTTVRLLLNDVSEYRTYLELKKQRFKCKSCQRTFVADT
                     SVAEKHCFISQKVRWSVIARLKENTSMTEIARQKNISTSSVYRVMKRFYRPLNPFKQT
                     LPKVLCFDEFKSVRSVASAMSFIMMDGQSHALLDIVENRRLPYLERYFSRFSLATREA
                     VQLIVIDMYAPYVSLVKKLFPNAQLIIDRFHIVQHIGRTFLNHRVKETTVRLKGNSHS
                     QRGLGKKLKRYWKLLQKEEAKLNYEKRVWRASFKDYLTESEIADRLLAACPNLRQGYQ
                     LYQDILYAVKKRDQSLFEDCLTRDISGLPETYTTTLRTFKKFLPQIQNALHYSYSNGP
                     LECLNNHIKVLKRNAYGFRNFYNFKLR"
     gene            1575..2300
                     /locus_tag="ACG8KR_002455"
     CDS             1575..2300
                     /locus_tag="ACG8KR_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0201773.1"
                     /translation="MKIIRYATARLIYYKKQTILYCLFSSFSAFLLIACLTLYHSQSA
                     LANQVQDRLSFIGASASTELLPALELASRFYIKGIFSLFFLFAFLFIFFFYYSVRQNK
                     QELINWRLTGLSKTKLFLFIFWQLFFPLFLCCILVLLWIVSFQSVYEALLQRINFLVL
                     KTTELPDIRDILTSVSGLTIPMDQQTFFTIDFQNDLFFTDILKGFAQTLIGLSGCCLI
                     LSLFPFTFFIHHLQSRRPGRNET"
     gene            2290..2799
                     /locus_tag="ACG8KR_002456"
     CDS             2290..2799
                     /locus_tag="ACG8KR_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0201774.1"
                     /translation="MKPEYLIHAEHLTVILCPLEKQHKILEHLKKEVLVAKLDNFAII
                     QKNWPMFPYLNLKDHVLLDVPEKEIKQDRLAYQEKLDISPSLLNCAAAELTSFEKVKL
                     QLLHALLSKRNNIVIEDTFDELSVLEIQELLHLLSYLAHEENQGILLFTHDATIAQSP
                     YIDRLEPAG"
     gene            complement(2869..4317)
                     /locus_tag="ACG8KR_002457"
     CDS             complement(2869..4317)
                     /locus_tag="ACG8KR_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFQ0201775.1"
                     /translation="MRYLYQQLLAFCGMVALILLIVGVSFTQLTKQTIEENNYQQLFG
                     YAESVEKTTQTYADTLPQFSQDQAFQNALLLTEQVLTEQNVNFIFIDKNERVIYPTNT
                     ALLNFTLTADQWKNLRNGRQEKFTSNKNILGQAQATSYALVPFNLNHEFYGALVVSQP
                     ARNIDNSVRPITLNLFKGFIFSSIVAVIASYAFAAFQVKRINRLRNATKEVTNGNFDV
                     QLPVHDKDEFDELADDFNKMTNSLKESQAEIEEQENRRRQFMADASHEMRTPLTTING
                     LLEGLEYNAIPENQRENAIKLMKNETERLIRLVNENLDYEKIRTNQISMVIKKFNGTE
                     TLKNIVAQLEAKAEAAGDTLTLKAVDDIDVYADYDRFVQIMVNIIQNAIQFTENGQIT
                     ITLEKGYLETIITIEDTGIGMSEQQMKSIWDRYYKVDPSRKNTKYGESGLGLPIVQQL
                     VRLHKGKLEVESELGKGTKFAVRLPDVEIKKE"
     gene            complement(4317..5033)
                     /locus_tag="ACG8KR_002458"
     CDS             complement(4317..5033)
                     /locus_tag="ACG8KR_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HFQ0201776.1"
                     /translation="MNILMIEDNQSVSEMMQMFFLNEGWEATFKYDGKEGLDAFLENP
                     QHWDMITLDLNLPTLDGMLVGREIRKVSSTVPIIMLTARDSESDQVIGLEMGADDYVT
                     KPFSPLTLIARMKALHRRSELAEAREEAAVSDDHFDIETSHFKMNTKTREAYLDNRPI
                     EGLTPKEFDLLFTLAKKPRQVFSREQLLELVWDYQYFGDERTVDAHIKKLRQKIEKVG
                     PQVIQTVWGVGYKFDDSGVA"
     gene            complement(5014..5364)
                     /locus_tag="ACG8KR_002459"
     CDS             complement(5014..5364)
                     /locus_tag="ACG8KR_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HFQ0201777.1"
                     /translation="MIFFSIGILFLIIGSLFRILPSKGNLPFYGYHSPLAAKTDAHWR
                     LAQKTSGNWFFLMGLLMALIGYYLKTSGHTNYFLIEMLLLVFPIMPIFIMTEKKLQKY
                     DLETGGNDNEYFND"
     gene            5508..6281
                     /locus_tag="ACG8KR_002460"
     CDS             5508..6281
                     /locus_tag="ACG8KR_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFQ0201778.1"
                     /translation="MSNYRAITFFDLDGTLLDAKSKITPEVAKAMNALKENNVLPVIA
                     TGRTEAEIHSIMEDAGITSAVTMNGSFIRVDGEEIYSQTFSSEECQKMYDHVQLNGHE
                     LSYYNDQKIWCTGHSQTMINAYDFIHSDVPEIDPLGFKENTVNMLLILSQSGDEYYHE
                     HFPELTFYRNGPYSIDTVKKGISKGAGVKRLQKELQLEKVPTFGFGDGPNDFALLEAC
                     DNKIAMDNAYDGLKELSTFITKKNTEGGIVHALKYFDLI"
     gene            6452..6818
                     /gene="ssrA"
                     /locus_tag="ACG8KR_002461"
     tmRNA           6452..6818
                     /gene="ssrA"
                     /locus_tag="ACG8KR_002461"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(6864..>7089)
                     /locus_tag="ACG8KR_002462"
     CDS             complement(6864..>7089)
                     /locus_tag="ACG8KR_002462"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0201779.1"
                     /translation="GTCLNHNCVFPSISIKVHLKKSVRTKVLFVNLKIDFPEYVLGIS
                     KCLNVFLNAKSMQKILYFSTFLSLTKIKKR"
BASE COUNT         2069 a         1387 c         1266 g         2367 t
ORIGIN      
        1 cgtagtttga aattgtaaaa gttgcgaaag ccgtacgcat tgcgttttaa tactttgatg
       61 tggttgttta agcattccaa tggtccgttt gaatagctgt agtgaagcgc attttggatc
      121 tgtggcagaa actttttaaa cgttcgtaaa gtggttgtat aggtttctgg cagaccgcta
      181 atgtctctag ttaaacaatc ctcaaatagt gactgatctc ttttctttac cgcataaaga
      241 atgtcttggt ataactgata gccttggcgt aagttgggac aagcagctaa cagtcgatcg
      301 gcaatctcac tttcagtcaa ataatcttta aaactggcac gccaaacccg tttttcatag
      361 tttaactttg cttcttcttt ttgtaggagt ttccaatagc gcttcaattt tttccctaac
      421 cctcgttgag agtgggaatt tcctttaagt cgtaccgtgg tttctttcac tcgatgattc
      481 aagaaagttc tgccaatatg ttggacgatg tggaaacgat cgatgatcaa ctgagcattc
      541 gggaaaagct ttttcaccaa cgaaacataa ggcgcataca tatcaataac aatcaattga
      601 acggcttcac gagtcgctaa tgaaaaccga gaaaaatagc gttctaaata aggcaagcgt
      661 cgattttcga caatatccaa taatgcatgg ctttgtccgt ccatcatgat aaaactcatt
      721 gcactggcaa cacttcgaac cgatttgaat tcatcaaagc ataagacctt tggcaaagtc
      781 tgtttgaaag gattcaatgg gcggtagaat cgcttcatga cacgatagac cgaagaagtg
      841 gaaatatttt tctgacgagc gatttccgtc atggaagtat tctcttttaa gcgagcaatg
      901 acagaccaac gaactttttg actaatgaaa cagtgttttt cagcaacaga ggtatccgct
      961 acgaatgttc tttggcaaga cttacattta aatcgttgtt tcttcagctc cagataagtg
     1021 cgatactcag acacatcatt cagtaacaac cgaacggttg tcgtccccca acgaatgatc
     1081 ctagagggga cgacacaaag acaatgggaa cactcttctg gcttgtacag aagagttcct
     1141 ttgtaaatca agcaaatttg ccctttgtat ttttctttag ttaaacaatt ttcctcgaaa
     1201 tagatattta ggtctagaat atttagggta tctttgataa gctgagtatg agacatcttg
     1261 aatcctccta tgaattggta gccaaattaa ttctaaagga tttcagggtg tcttttctat
     1321 atttttttat acaaaaaaga tgttggtgaa gttcaccaac atcaaaaagt atagagccca
     1381 aaattattgt cgctgctcct cttttcgaat aaacagtgtt caaaagcttg catctacggt
     1441 aatagaatag ggcagcttct tcacaacctc ttgcttttta caaaatattt tctggtttta
     1501 attcgtgatt tcactaaagg aatttcatac tttattcata ttcattttct atacttttta
     1561 tagaggtgaa aagtatgaaa ataatccgct atgctactgc tcgattaatt tattacaaaa
     1621 agcagacaat actctattgt ttattttcaa gcttttctgc ttttttattg atagcatgcc
     1681 tgactcttta ccattcgcaa tcggctttag ccaatcaagt ccaagatcgg ctttcattta
     1741 ttggcgcttc tgcatctaca gagcttcttc cggctttaga attagctagc cgattttata
     1801 taaaaggcat attcagttta ttttttctat ttgccttcct ttttatcttt ttcttttact
     1861 attcagtcag gcaaaacaag caggaattga tcaattggcg cctgactggt ctttctaaaa
     1921 ccaaattatt cctttttatt ttctggcagc tgttctttcc tctctttctt tgttgtatct
     1981 tggttttatt gtggatcgtc agcttccagt cagtttatga agcgcttctt caaaggatca
     2041 attttcttgt cttaaagact actgaactgc cagatatccg tgatatcttg acttcggtaa
     2101 gcggacttac aattccgatg gatcaacaga ctttttttac catcgatttc caaaatgatc
     2161 tttttttcac agatatactt aagggatttg cccaaactct tattgggctc agtggatgct
     2221 gtctcattct tagtctattc cctttcactt tttttatcca tcatttgcaa agtaggaggc
     2281 ctggacgtaa tgaaacctga atatttgatt catgctgaac acttgaccgt tattttgtgt
     2341 cctctcgaaa aacagcataa aatattggag cacttgaaaa aagaggttct tgttgcaaaa
     2401 ctggataatt ttgcgatcat ccaaaaaaat tggccgatgt ttccttatct gaatcttaaa
     2461 gaccatgtcc tgttggatgt accggaaaaa gaaatcaaac aagatcgttt ggcctatcaa
     2521 gaaaaacttg atatctctcc ttccttgctt aattgtgcgg cagctgagtt gacgtctttt
     2581 gaaaaagtaa agctgcaatt acttcatgcc ttgctttcca agcgaaacaa tattgttatt
     2641 gaagatacat ttgatgaact tagtgtattg gagatccaag agctactcca cttgctttct
     2701 tatttagctc atgaagaaaa tcaaggtatc ttactcttta ctcatgatgc tactattgct
     2761 caaagtcctt atattgatcg tttagagccc gcgggctaaa tcaatcattt tatagtaaaa
     2821 acaagcggta tagaagatcg attcatgatc ttttataccg cttgtttttt actctttttt
     2881 gatttctacg tcaggcaaac gaacagcgaa ttttgttcct tttcctagtt cgctttctac
     2941 ttctaatttt cctttatgca agcgtacaag ttgctgcacg attggtaaac ctaatcctga
     3001 ttcaccatat tttgtatttt ttcttgaagg atctacttta taataacgat cccagatact
     3061 tttcatttgt tgttcagaca ttccaattcc cgtatcttca atcgtgatga tcgtttcaag
     3121 atagcctttt tctaaagtaa tcgtgatttg gccgttttca gtaaactgta tcgcgttttg
     3181 aataatattg accatgattt ggacaaatcg gtcataatca gcgtaaacat caatatcatc
     3241 aacggctttc aatgtcaatg tatctcctgc tgcttctgct ttagcttcta actgtgcaac
     3301 aatatttttc aatgtttctg ttccattgaa ttttttaatg accatactga tttgattcgt
     3361 acggattttt tcataatcca agttttcatt caccaaacgg atcagacgct ctgtttcatt
     3421 tttcatcaat ttaatcgcgt tttctcgctg attttcaggg attgcattgt attctaaccc
     3481 ttccagaagt ccgttgattg tcgtaagagg tgttctcatc tcgtgagaag catctgccat
     3541 aaattgtctt cgacgatttt cttgttcttc aatttctgct tgagattctt tcaatgaatt
     3601 cgtcatttta ttgaaatcat ctgctaactc atcaaattca tctttgtcat gtacggggag
     3661 ctgtacatca aaattaccat tcgtcacttc tttcgtcgca ttccttaacc gattgatacg
     3721 cttaacttgg aatgctgcaa aagcatagct ggcaataact gcaacgatac tggaaaaaat
     3781 aaatcctttg aacaggttca gtgtaattgg acgaacgctg ttatcgatat ttcgcgcagg
     3841 ttgactaact acaagtgctc catagaattc atgattcaaa ttaaatggca caagtgcgta
     3901 agaggtcgcc tgtgcttgtc ctaaaatatt tttattcgat gtgaattttt cttgacgtcc
     3961 attcctcaga tttttccatt gatcagctgt taaggtaaaa ttcagtaaag ctgtattagt
     4021 cggataaatg acacgttcgt ttttatcgat aaatataaag ttcacatttt gttctgtcag
     4081 tacttgctct gtcagcagca atgcattttg aaacgcctga tcttgactaa actgcgggag
     4141 cgtatcggca taggtttgcg ttgtcttttc aactgattca gcatatccaa acagttgctg
     4201 gtaattgttt tcttcaatcg tttgttttgt cagttgtgta aaagaaactc caacgatcaa
     4261 taagatcaac gcgaccattc cgcaaaaagc taagagttgc tgatacaaat aacgcattag
     4321 gcaacaccag aatcatcgaa tttgtagccg actccccaaa cagtttggat gacttgtggt
     4381 ccgacctttt cgattttttg tcgtaatttc ttgatatggg cgtccactgt ccgttcatca
     4441 ccaaagtact gataatccca gactaattca agcaactgct cccgagagaa tacttgacgt
     4501 ggtttttttg ctaaagtaaa caatagatcg aactctttag gcgtcagtcc ttcaattggc
     4561 cggttgtcca gatacgcttc tcgtgttttc gtattcatct tgaaatgact agtctcgata
     4621 tcaaaatgat catctgaaac tgctgcttct tctcttgctt ctgccaactc actacggcga
     4681 tgcaaagctt tcattcgagc aataagcgtc agaggactga aaggttttgt gacataatca
     4741 tctgccccca tctctaagcc gatcacctga tcgctttcag aatctctagc tgtcagcatg
     4801 ataatcggca cagtacttga aactttgcgg atctctcttc ctactagcat accgtccagt
     4861 gtgggcaaat tcaggtctag tgtaatcata tcccaatgtt gtgggttttc taaaaaggca
     4921 tcgagccctt cttttccatc atacttgaaa gttgcttccc aaccttcatt taagaaaaac
     4981 atctgcatca tttcagaaac agattgatta tcttcaatca ttaaaatatt cattatcgtt
     5041 ccctccagtt tccaggtcat atttctgtaa tttcttttct gtcataataa aaataggcat
     5101 aattggaaat actagcagaa gcatttcaat cagaaaataa ttcgtatgcc cagatgtctt
     5161 taaataatag ccgatcaatg ccatcagtaa gcccataaga aaaaaccagt ttccgcttgt
     5221 cttttgagcc agccgccaat gagcgtctgt ttttgcggca agaggcgaat gatacccata
     5281 aaaaggcaga tttccttttg agggcagaat ccgaaaaaga ctgccaatta ttaagaataa
     5341 gataccaata ctgaaaaaaa tcattctttc cctcctaaat tctcttctta tagtatactc
     5401 taaattgttg actgaaccaa tatgttcagg aattcttcac gaaaggtttg tcttttgttc
     5461 attctttgtt aagataaact tattccaata aaaaaggagt tttatttgtg tcaaattatc
     5521 gtgcaatcac tttctttgat cttgatggaa cattactaga tgcgaaatca aaaatcacac
     5581 ccgaagtggc aaaagctatg aacgcattaa aagaaaacaa tgttctcccc gtcatcgcta
     5641 caggacgtac agaagcagaa atccactcta tcatggaaga tgcgggcatt acttctgcgg
     5701 tcaccatgaa tggttctttc attcgagtag acggcgaaga aatctattct caaacatttt
     5761 cttcagaaga atgtcaaaaa atgtatgatc atgtacagtt gaacggtcat gaactttctt
     5821 actataacga tcagaaaatc tggtgtaccg gtcatagcca aacaatgatc aatgcttacg
     5881 acttcattca ttcagatgtt ccggaaattg atcctttagg ctttaaagaa aacacagtaa
     5941 atatgttgct gatcttatct caatcaggtg acgaatacta tcacgaacat tttcctgagc
     6001 taacttttta tcggaatggt ccttactcga tcgatacagt gaagaaagga atctcaaaag
     6061 gggcaggtgt caaacgtctg caaaaagaac ttcagctgga aaaagttcct acttttggat
     6121 ttggcgatgg tccgaatgat tttgccttgt tggaagcttg cgacaacaaa atcgctatgg
     6181 acaacgccta tgacggatta aaagaacttt ctacctttat cacaaagaaa aatacagaag
     6241 gcggaattgt ccacgcattg aagtattttg atctgattta agatcttttt ttgaagctac
     6301 ctcttttctt aagaaaatcg cttatttttt cttaagaaag gggcagttat tatataaata
     6361 atgggatatg attcgctttt ttattgaatt tatcccaata tatgctatac taattttgta
     6421 tgaaaaatac acgtaaccct tgttagctaa cggggacgtt acggattcga caggcacagt
     6481 cgagcttgaa ttgcgtttcg taggttacgt ctacgtaaaa acgttacagt taaatataac
     6541 tgctaaaaac gaaaacaact cttacgcttt agctgcctaa aaacagttag cgtagatcct
     6601 ctcggcatcg cccatgtgct cgagtaaggg tcctaacttt agtgggatac gtttcaactt
     6661 tccgtctgta agttgaaaaa gagaacatca gactagcgat acagaatgcc tgtcactcgg
     6721 caagctgtaa agtgaatcct taaatgagtt gactatgaac gtagattttt aagtggcgat
     6781 gtgtttggac gcgggttcga ctcccgccgt ctccatatat gtatggttga gacagataaa
     6841 catgtatgaa aaacgttgtt aaatcaacgt ttttttattt tggtcaagga gagaaaagta
     6901 gagaaataca aaattttttg cattgatttt gcattcaaaa aaacattcaa acatttagaa
     6961 atccccaata cgtattcagg aaaatctatt ttaaggttta caaaaagaac ttttgttcgt
     7021 acactctttt tgaggtgaac ttttatgctg atggaaggaa aaacacaatt atggtttaag
     7081 catgttcct
//
LOCUS       DAZKLD010000137         6945 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.138, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000137 DAZKLD010000000
VERSION     DAZKLD010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6945)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6945)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6945
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.138"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..734
                     /locus_tag="ACG8KR_002463"
     CDS             <1..734
                     /locus_tag="ACG8KR_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="BREX system P-loop protein BrxC"
                     /protein_id="HFQ0201780.1"
                     /translation="IKNFHKQNSTQKKIWENAQDKIRLIEKSRRQTSSQEIDQLIHEM
                     KTILKKADPYSDMMKLNELIQEFIEQYNQLLELESQEILNKLTEEKQATFKLLEGKDF
                     KDQFVPIVDQEFQELKAKIEQANTIDDLVFLSLNVAEQYLIFNQRFDNEENRRRQEEQ
                     ARKQIEAANLIRETEKDEIDSAAKAEEIAIVKPTIQQKTVPMRTLNVHVKTIKSAADI
                     DELTAEINQKLKEQLQENTEITIQF"
     gene            798..4430
                     /gene="pglX"
                     /locus_tag="ACG8KR_002464"
     CDS             798..4430
                     /gene="pglX"
                     /locus_tag="ACG8KR_002464"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BREX-1 system adenine-specific
                     DNA-methyltransferase PglX"
                     /protein_id="HFQ0201781.1"
                     /translation="MDKKAIKNFAVNARRKLIDEVMTKAKRLGIDESKIEDVKKIDSN
                     LQEIEKSGIRIQGKEIAQRNKLIAELKQRASVTSYSEAFQELIEEVAYTWFNRLIAIR
                     FMEVNDYLPDTYRVLSSEISGKIEPDIITDIYDADIFEELSSNEQQQVRTWKTDNSAE
                     AMDKLYQLIFIKICNQLNDYLPELFEEVEDYTELLFTASYIKKDGVIADLLSIPEDDF
                     YVQAGGQVEIIGWLYQYYNSEPHNQVVNIYKGIVKKQDIPAATQLFTTDWVVRYMVDN
                     SLGKYWLDHYPNSELRNKLKYLIPDKYAFEQATSVSNLKPEELKIIDNAMGSGHILVY
                     AFDVLMEIYLTLGYSERDAARAIVEYNLYGLEIDKRAYQLSYFAIMMKGRQYDRLFLK
                     KSLSPNLREFIDSPKVTDEYQERISELNNDKYSERWNEYKELIFDQFENGKELGSIIK
                     LVDKNNQAITKEYLGKVREYISKFNEFTNMDILYGMKEIQEKYLHILDVAEMLVDKYV
                     AVVTNPPYLNKMDKKLKKYVNDNYSDVKKDLFSVFIKLNSQMLIDNGYASFMTPFVWM
                     FISSYEKLRSFLINERNISSLIQMEYSAFEEATVPICTFTIKNSNEQDGSYIKLSDFK
                     GGMEVQKIKTLEAIHQHGECDYFYSTNQDNFKKIPGMPIAYWASDNVITDFAQGKLTN
                     DVADAKQGLATGNNNKFLRYWFEVEFNKIKFDAKTLEDAKKSRKKWFPTTKGGRFRKW
                     YGNNEYVVNWENDGDEIKNYVNENGKLKSRPQNLNYYFKKGITWSTLSSGQVSFRLQN
                     QAIFETKGSVLFSKDITDFEEIFGYVNSKLVNHFLTFISPTLDYHEGPMGKLPFLKVT
                     SERKLKKIREKVDENIDIARKDYNALETSYEFRRNILLSYMFKKDLSEAFNCLSRQLV
                     NQFNTLKSNEEELNDIFINHYSLEKELTKEVQDEYISARLPDKAREIKDFISYSIGCI
                     FGRYSLDEDGLIFAGGEWDPSRYQKFKPDTDNVLLITDDEYFKDSQIDIVNRFIDFVE
                     ITFGKETLEENLQFIADTLGGKGTSREIIRRYFVQDFYKDHLKTYSNRPIYWQLDSGK
                     QNGFKALMYLHRYTPDQLGKVRTEYLHELQKAYDNRIELRQQQISTSDNPKERNAMTK
                     EIVKIQKQLKEVREYDEKLGHLALKRIELDLDDGVIVNYDKLQRDPKTGEKFQIFAKG
                     VKEPKK"
     gene            4546..>6945
                     /gene="pglZ"
                     /locus_tag="ACG8KR_002465"
     CDS             4546..>6945
                     /gene="pglZ"
                     /locus_tag="ACG8KR_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BREX-1 system phosphatase PglZ type A"
                     /protein_id="HFQ0201782.1"
                     /translation="MTDINTQQIEEKLNQRFPNYGCRKIIFWFDSNQDFLEEIDSLVI
                     QNAKIYRLEKDAQFKTKRLLEYEDPETNYLIYAPFPRWDDKDEDNHLLSVLNYSEEFS
                     ADRLAIIMNELAIPLRFHQTVKQYRNFFNAKDRMRSFGEFSTHMTINKSETIELMIMA
                     VLLKSKSIRLNDLLRVVLKSFTESRENPLRELDKYQLTAKFWEFIQMKFGFTDSQPTL
                     EKLTMCLYLNYFYYQIDQEMPKKFKGFEILHLKANIITFMDQFMNDTRYMESFEQLSK
                     KVYQAIDGDGLLDSIEIDDLIEADVFSEIHAKILMFYAERLKASDTTTKIAGQTIFDS
                     LKQREKMHFASDYIYEYQLLKHAYYLVDKNRLCLPMNMDNLLTQYEKEMYWVDTHYRK
                     FIWNSDHIEEKQKYGQLYRLVEANYSKFLDQSGQIWNQTFEFKNRPSIRKFYNQAVMK
                     RSVKTVVIISDAFRYELGMNLERKLVREKKYSTNMASFLSVLPSVTEFGKAALLPNNQ
                     LTYNEGTEVLIDGKKTNGLSNRNAILQQRDANAVALNYDDVVKMNKAEVREVFNGKSV
                     IYIYHDQVDRTGDHSNENRVFEACDLAISEISRLINRLHNDGSIYRFIVTADHGFIYK
                     RSHVEEVDKIENPSRNEKDRVERRFILSEEEYEIIGTKNYSLGDSLGNDDTRNICVPI
                     TSSIFKKAGGGQSYVHGGSSIQEMLVPVLEITVAKGSNAKEPVAIELMTSNRRITGLS
                     TTLEFYQKNAISDVYDPASFWLYFEDEYGERISNEEIYVADSKTEDANQRFTKFVFEF
                     VN"
BASE COUNT         2616 a          937 c         1312 g         2080 t
ORIGIN      
        1 aaattaaaaa tttccacaag caaaatagta ctcagaaaaa aatctgggag aatgcacaag
       61 ataaaattag attaatcgaa aaaagtcggc gtcaaacttc tagccaagaa attgatcaat
      121 tgattcatga gatgaaaacc attttgaaaa aggctgatcc ttattcagat atgatgaaac
      181 taaatgagtt gattcaagaa tttattgaac aatataatca actgttagag ttggaaagtc
      241 aggaaatatt aaataaatta acggaagaaa aacaagcaac atttaaatta ttagaaggaa
      301 aagattttaa ggatcaattt gtaccaatag ttgaccaaga atttcaagaa ctgaaagcaa
      361 aaattgaaca agcgaatact atagatgatc ttgttttctt atcattaaac gtagcagaac
      421 agtatttgat ctttaatcaa cgtttcgata atgaagaaaa ccgcagacgt caagaagaac
      481 aagcacgtaa acaaattgaa gcagcaaact tgattcgtga aactgaaaaa gatgaaatag
      541 actctgcagc aaaagctgaa gaaatagcga tagtaaaacc tactattcaa cagaaaacag
      601 taccaatgag aacacttaac gttcatgtta aaactattaa gtcagcagca gatattgatg
      661 agttaacagc tgaaattaat caaaaactga aggaacaact tcaagaaaat acagaaatca
      721 cgattcaatt ctagtgaaaa gacaggcata aaaaggtgcc tgtcttttca aaggttacat
      781 atagaaggga aagaaaaatg gataagaaag cgataaagaa tttcgcggtc aatgcccgta
      841 gaaaattaat tgatgaagta atgactaaag caaaacgttt aggaattgat gaatcaaaaa
      901 ttgaagatgt gaagaaaatt gatagcaatt tacaagaaat cgaaaaatca ggaataagaa
      961 ttcaaggaaa agaaatagcg caaagaaaca aattgattgc agaactaaaa caacgtgctt
     1021 ctgtcacttc ttattcagaa gcttttcaag agttaatcga agaggttgct tatacgtggt
     1081 ttaaccgatt gattgccatt cggtttatgg aggtaaatga ttatttacct gacacgtatc
     1141 gtgtattatc tagtgaaata agtgggaaaa ttgagccaga tattattaca gatatttatg
     1201 atgccgatat ctttgaagaa ttatctagta atgaacagca acaagtcaga acttggaaaa
     1261 cagataattc agcagaagca atggacaagc tgtatcaatt gatttttata aaaatctgta
     1321 atcaattgaa tgattactta cctgagctat ttgaagaagt agaagattat acagaacttt
     1381 tgtttacagc ttcttatatt aaaaaagatg gtgtaatcgc tgatctttta agtattccag
     1441 aggatgattt ctatgttcaa gctggtggac aggtagagat tattgggtgg ttgtatcagt
     1501 actataatag tgagccacat aatcaagtgg tgaatattta taaagggatt gtcaaaaaac
     1561 aagatattcc ggcagcaact caactattta ccacagattg ggtcgttcgt tatatggttg
     1621 acaatagttt aggaaagtat tggttagatc attatcccaa tagtgaatta agaaataagt
     1681 tgaaatactt aattccagat aaatatgctt ttgaacaagc aacatctgta agtaatttaa
     1741 aaccagaaga actaaaaatt atcgataacg caatggggtc tgggcatatt ttagtttacg
     1801 ccttcgatgt attgatggaa atttatctta cgttaggata ttctgagcgt gatgctgcac
     1861 gtgcaatcgt tgaatacaat ttatatggtc ttgaaatcga taaaagagcg tatcaattgt
     1921 cttattttgc aattatgatg aaagggcgtc aatacgatcg tctttttttg aaaaaaagtc
     1981 tatcgccgaa tttaagagag tttattgatt ctcctaaagt gacagacgag tatcaagagc
     2041 gtattagtga attgaacaat gataagtata gtgaacgctg gaatgaatac aaagagttaa
     2101 tttttgatca atttgaaaat gggaaagaac ttggatcaat cattaagtta gtcgataaaa
     2161 ataatcaggc aataactaaa gaatacttag gaaaagtaag agagtatata tcaaaattca
     2221 acgaatttac gaatatggat attctttatg ggatgaagga aatacaagaa aaatacttgc
     2281 acattcttga cgtggctgaa atgctagttg ataaatatgt agcggtagtc actaatccgc
     2341 cttatcttaa taaaatggat aagaaattaa aaaaatatgt aaatgataac tattctgatg
     2401 tcaaaaaaga tctattttct gtgtttatta aattgaatag ccaaatgctg attgataatg
     2461 gatatgcttc ctttatgacg ccatttgttt ggatgttcat ttctagctat gaaaagttac
     2521 gctcattttt aattaatgaa agaaacattt ctagtttgat tcaaatggag tattcagctt
     2581 ttgaagaagc aaccgttcca atttgtacgt ttacgataaa aaattcaaat gaacaagatg
     2641 gaagctatat aaaattatca gattttaaag gtggaatgga agttcaaaaa attaaaacgt
     2701 tagaagcaat tcatcagcat ggtgaatgcg attattttta tagtactaat caagacaact
     2761 tcaaaaaaat cccaggaatg ccgattgctt actgggcaag cgataacgta atcactgatt
     2821 ttgctcaagg aaaattaact aacgatgttg cagatgcaaa acaagggttg gcaaccggaa
     2881 ataacaataa atttttgcgg tattggtttg aagtagaatt taacaaaata aagtttgatg
     2941 ctaaaacatt agaggacgct aaaaagtcta gaaaaaaatg gtttcctaca actaaaggtg
     3001 gtcgttttag aaaatggtat ggaaacaatg aatacgtggt taactgggag aatgatggag
     3061 atgaaataaa aaattatgtt aatgaaaatg ggaagttaaa atctagacca caaaatctta
     3121 attactattt taaaaaggga attacttggt cgacactttc atctggacaa gtatctttta
     3181 gattgcagaa tcaggcaatt tttgaaacta aaggatctgt tctgttctca aaggatataa
     3241 ctgactttga agaaatattt ggctatgtaa actctaaatt agttaatcac tttttaactt
     3301 ttatttctcc caccttggat taccatgaag gaccaatggg aaaactacct tttcttaaag
     3361 ttacatctga gagaaaatta aaaaaaataa gagaaaaagt ggatgaaaat atcgatatag
     3421 ctagaaaaga ctataatgct cttgaaacgt catacgagtt tagaagaaac attttgttaa
     3481 gttatatgtt taaaaaagat ctctcagagg catttaactg tctaagtagg cagttggtta
     3541 atcaatttaa tactctaaaa tcgaatgaag aggaactgaa cgacatattc attaatcatt
     3601 attctcttga aaaagaatta acaaaagagg ttcaagatga atatatctct gcaaggttac
     3661 ctgataaggc gagggaaatt aaggatttta tttcttattc aataggttgt atcttcggtc
     3721 gttactccct agacgaagat ggtctcatct tcgctggcgg cgaatgggat ccttcccgct
     3781 atcaaaaatt taaacctgat actgataatg ttttactcat caccgatgat gagtatttca
     3841 aggatagcca aattgacatt gtcaatcgtt tcattgattt cgttgagatt acttttggta
     3901 aagaaaccct tgaagaaaat ctccagttta tcgctgacac tttaggtggc aaagggacaa
     3961 gtcgagaaat tattcgtcgt tacttcgtac aagattttta caaagaccat ttaaaaacat
     4021 actccaatcg accaatttat tggcaattgg actcaggaaa acaaaatggc tttaaagcgt
     4081 taatgtactt gcatcgctat acaccagatc aattaggaaa agtacgtact gaatacctac
     4141 acgaactaca aaaagcgtat gacaatcgaa tcgaattgcg tcagcaacaa atttcaacaa
     4201 gtgataaccc aaaagaaaga aatgcaatga caaaagaaat tgtaaagatt cagaagcaat
     4261 taaaagaagt tcgtgagtat gacgagaagt tgggtcactt agcattaaaa cgaattgaat
     4321 tagacttaga tgatggtgta attgtcaatt atgataagtt gcagcgtgat cctaaaaccg
     4381 gcgagaaatt ccaaattttt gcaaaagggg tcaaagaacc aaaaaaatag ttatctaaga
     4441 aagttgtttt ctaacaactt tcttttttat tcttaaattg cgtgcgaaat tatccaaatt
     4501 atgtcaatat aaggggtata gaaagagaac gattaggtga gattgatgac agatataaat
     4561 acgcaacaaa ttgaagaaaa attaaatcaa cgttttccta attatggatg taggaaaatc
     4621 attttttggt ttgattcaaa tcaggatttt cttgaagaaa ttgactcatt ggtcattcaa
     4681 aatgcaaaaa tctatcgatt agaaaaggac gcacaattta aaactaagcg tttattggaa
     4741 tatgaagatc ccgaaactaa ctatttaatc tatgcgccat ttcctcgttg ggatgataaa
     4801 gatgaagata atcatttgtt gagtgtattg aattattcag aggaatttag tgcagaccgc
     4861 ttagcgatta tcatgaatga attggctatt ccacttagat ttcatcagac agttaaacag
     4921 tatcgtaatt tctttaatgc aaaggatcgc atgagaagtt ttggtgaatt tagtacgcat
     4981 atgacaatta ataaatcaga aacgattgaa ttaatgataa tggctgtttt gttgaaatcg
     5041 aaaagtattc gattaaatga tttactgcgt gtagttctaa aaagctttac agaaagtagg
     5101 gagaatcctt taagggaact tgataagtat cagttaacag ctaaattttg ggaatttatt
     5161 caaatgaaat ttggctttac ggatagccag ccaacattgg aaaagctgac gatgtgtctt
     5221 tatttgaact atttttatta tcaaattgat caagagatgc cgaagaagtt caaaggattt
     5281 gaaatactac atttgaaagc aaacattatt actttcatgg atcagtttat gaatgataca
     5341 cgttacatgg aaagctttga acagctatct aaaaaagtat atcaggctat tgatggtgat
     5401 ggattattag acagtataga aattgatgat ctaattgaag cagacgtatt ttctgagatt
     5461 catgcaaaaa tattaatgtt ttatgcagaa cgattaaagg cttctgacac tacaacgaaa
     5521 attgcaggac aaacaatttt tgatagttta aagcagcgcg aaaaaatgca tttcgcatcg
     5581 gattatattt atgagtatca attattgaaa catgcttatt atcttgtgga taaaaacaga
     5641 ttgtgcttgc cgatgaacat ggataatttg ctaactcaat atgagaagga aatgtattgg
     5701 gttgatacgc attatcggaa atttatttgg aatagtgatc atatagaaga aaagcaaaaa
     5761 tatggccaat tgtatcgatt agttgaagca aattattcga agtttcttga tcaatcagga
     5821 caaatttgga atcaaacttt tgaatttaag aatcgtccat ccattcgaaa attttataat
     5881 caagctgtaa tgaaacgctc tgttaaaact gtagttatta tttcagatgc ttttcgatat
     5941 gagttaggga tgaatttaga acgtaagtta gttagagaaa agaagtattc aacaaacatg
     6001 gctagctttc tttctgtatt accttcagtt actgaattcg gtaaagccgc cttattacca
     6061 aacaatcaac taacttacaa tgaaggaaca gaagtactga ttgatgggaa aaaaacgaat
     6121 ggtttatcca atcgaaatgc gattctccag caacgagatg caaatgctgt agctttaaat
     6181 tatgatgatg tggttaagat gaataaggct gaggtacgag aagtatttaa tggcaaatca
     6241 gtcatttata tttatcacga tcaagtggat agaacaggtg atcactcgaa tgaaaatcga
     6301 gtttttgagg cctgtgattt agcgataagc gaaatttccc gacttattaa tcgcttgcat
     6361 aatgatggca gtatctatcg ttttatagtt acggctgatc atggatttat ttataaaaga
     6421 agccacgttg aagaagtaga taagattgaa aatccatcta gaaatgaaaa agatcgtgta
     6481 gaacgtcgtt ttattcttag tgaagaagaa tacgaaataa ttggtacgaa aaattactct
     6541 ttgggagatt ctttaggaaa tgatgacact agaaacattt gtgtcccgat tacttcaagt
     6601 atttttaaga aagctggcgg gggacaaagc tacgttcatg gagggtcatc tattcaagaa
     6661 atgctagtcc ctgttctaga aattacggta gccaaaggta gcaatgcaaa ggaaccagta
     6721 gcaattgaat taatgactag caaccgcaga attacaggat tatcaaccac gttagaattc
     6781 tatcaaaaaa atgcaattag tgatgtttat gaccccgcat ctttctggct ttattttgaa
     6841 gatgaatatg gtgaacgtat ctcaaatgaa gaaatctatg ttgcggattc aaaaacagaa
     6901 gatgcaaatc agcggtttac taaatttgtt tttgaatttg taaat
//
LOCUS       DAZKLD010000138         6794 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.139, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000138 DAZKLD010000000
VERSION     DAZKLD010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6794)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6794)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6794
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.139"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(137..550)
                     /locus_tag="ACG8KR_002466"
     CDS             complement(137..550)
                     /locus_tag="ACG8KR_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HFQ0201783.1"
                     /translation="MANVPSIRDMFPDFNDVFSPAFNDFLGVSSYPKVDLVENEKEYK
                     LTADMPGCDKEDTTVEYSDNTLTISANHESHTEDKEDGNYVRKERHSVSYKRSFYLPN
                     VDEEKITGTFKNGVLKLVLPKTAYQPKETKKIELN"
     gene            complement(1006..1491)
                     /locus_tag="ACG8KR_002467"
     CDS             complement(1006..1491)
                     /locus_tag="ACG8KR_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0201784.1"
                     /translation="MHKGIYAGLLGTVILFSACGYKTKNSVKESSAAISTTEVGSTKS
                     TSTTSITNTSTGNVDSQHSQSSIQSFSISSTSEMQESTVPSLPLENSDKALLFLIAHR
                     SELQSDDIVISFYQKIDRDYLFTASSKQIRSQGGSGSVGFYRVSPEGSITMTDANGTP
                     F"
     gene            complement(1630..1845)
                     /locus_tag="ACG8KR_002468"
     CDS             complement(1630..1845)
                     /locus_tag="ACG8KR_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HFQ0201785.1"
                     /translation="MKTLDAIALTLLIVGGLNWLLVGLFELDLVAMIAGGSTTIFAKI
                     IYIVVGICAIYCLKFFPMITRKVDERY"
     gene            2069..2869
                     /locus_tag="ACG8KR_002469"
     CDS             2069..2869
                     /locus_tag="ACG8KR_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFQ0201786.1"
                     /translation="MSDWLDIAGKVVIVTGGSSGIGRSIVENLLKQNAQVANFDVTEC
                     RIQHENLLSLKVDVSSKTDIEEGIYKVMKHFKTIDGLVNNAGINIPSLLIDRNHPKSK
                     YELSEQVFDKMIAVNQKSVYLMSQAVGRILVQKGSGVIVNLSSESGLEGSEGQSCYAA
                     TKAAMNSFTRSWAKELGKRNVRVVGVAPGILEETGLRTQEYEEALSYTRGISVEQLRN
                     GYSRTSTIPLGRSGKLQEVADLVCYYLSERSSYITGVTTNISGGKTRG"
     gene            2888..4756
                     /locus_tag="ACG8KR_002470"
     CDS             2888..4756
                     /locus_tag="ACG8KR_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HFQ0201787.1"
                     /translation="MTLVNRWYQLLKLLVTHKKVSIQEVQKEIKTSNQTIKKDIGELN
                     EEIAGIAKIIQKNKHFELEIYDFDFFDEIMQGKLKTASDFNSASKRIAYLLNRLIETE
                     DFLRMDDLSEEVGVSRGTVVKDLKTLKEMINDFDVKITGTPNKGLRIIGDELELRLLL
                     FYFVSPYFSETKTEQFLEEKQAIKHFQKKTNASKQEITLLTKVIGISLNRISSNYLLK
                     KPINRYSGCFEYIQEFNELINQLEEIYELTLSQYEKDFISFPLNIINTSITMIRKANS
                     DLRLYFDPMMMRIRRLDLIDLDESFLYQEMKQHLSLLINRVIFRYRLTDLFYGEIEKK
                     YPFSYQIASECIQALEAILQHKISKVEISYLTLYLELAFRYQKEHFSSKKVAIVHTTG
                     KGTALMIQRQIKRILGRDIQITHVSEEQYEKVDLNKYFAVFTTIPLKNVNEETPVIHL
                     TNLFDDSWLRAQWQKAKKKRLAGFHSTVFFYQIWKEKKTYQEYLNKLVAPLIRGYYVD
                     EEFEKRILKREYQQSTFVNKEFAFPHTTNQLSQHIVLSVGLITDQGLAETGGNGVIFL
                     FAIPEEMTAEVENELFELYDILFELIKMGIDEFIELAPTQESFLKLIKSKGERLHE"
     gene            4749..5240
                     /locus_tag="ACG8KR_002471"
     CDS             4749..5240
                     /locus_tag="ACG8KR_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HFQ0201788.1"
                     /translation="MNDMYLFIVFAASAYVLQAVISWKQLKHFNQTYSELRRKGKVAI
                     GKRPGKIRSGTIVMLAIDDHDQVLEVRKMQGVTSLAKFHSLPVYAGEDIHYIDRYHPS
                     VRAEDRLTQAALENAREVYLRIEAGNEKSEQPSLPFIAMLNNFRIWKSTIKEKRSDKR
                     WIS"
     gene            5228..5782
                     /locus_tag="ACG8KR_002472"
     CDS             5228..5782
                     /locus_tag="ACG8KR_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HFQ0201789.1"
                     /translation="MDIITKFAEGFMRLFQTGAETFISWMTGIVPVVLMLMVAMNTLI
                     AFLGEDRVTKVAKLSAKNPLTRYLVLPFLSAFMLGNPMSFTMARFLPEYYKPSYYAAQ
                     AQFCHTSNGVFPHINPGELFVWMGIAQGIETLGLNSMELAIRYLLVGLLMNFIGGWIT
                     DFTTGFVCRQQGIVLSKKVELSVD"
     gene            5800..>6794
                     /locus_tag="ACG8KR_002473"
     CDS             5800..>6794
                     /locus_tag="ACG8KR_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HFQ0201790.1"
                     /translation="MYGDIKIVRGNGGYGGPLIIRPTKDKHKFIYVTGGGERPMIVDK
                     IEELTGMESINGFKTSIPDSEIALAIVDCGGTLRCGIYPKKGIPTINIVSTGKSGPLA
                     QYITEDIYVSAVGLEQIQPLMETDDNKETVNTASPDKKYKYDTSKKITEQKAEQQNWI
                     AKIGMGAGKVVATFNQSAREAVDTMIRTVIPFMAFVSLLIGVIQGSGIGNVFANIMAP
                     LAGNAVGLTIIGFICSLPFLSPLLGPGAVISQVIGTLIGVEIGKGNIPPQLALPALFA
                     INTQNACDFIPVGLGLEEAKAETVEVGVPSVLYSRFLNGIPRVIIAWVASFGLYS"
BASE COUNT         2368 a         1086 c         1324 g         2016 t
ORIGIN      
        1 attttttacc aagagagatc ataacatctt tatcgtaaaa acaaaatatt tttttgctca
       61 ttttgaaaat ttaaacatac tcgttcttat ccaaatatat aaaaatcgcc cctcattcat
      121 cgagaggcga taagttctaa tttaactcaa ttttttttgt ttcttttggc tgataagctg
      181 ttttaggtaa taccaatttc agcacaccgt ttttaaaggt tccagtgatt ttttcttcat
      241 caacattagg aagatagaat gaacgtttgt aagagaccga atgtctttct ttccgtacat
      301 agttgccatc ttctttatct tctgtatggg actcatgatt ggcggagatc gtcaaagtat
      361 tatctgaata ttcaactgtc gtgtcttctt tgtcacagcc aggcatatca gcagttagtt
      421 tgtattcttt ttcattttcg actaaatcta cttttggata acttgaaaca cctaaaaaat
      481 cattgaatgc tggactaaac acgtcattga aatcagggaa catgtcgcga atgctaggta
      541 catttgccat ttttgatcat cctttcaata agtatttttt atttatccct atttacgtaa
      601 taactctatt ttgaaaaaaa tgcaagtata atggttagct ttgtgaatat ttcatctaat
      661 acgatacaaa ggctatcttt ctaatttgaa aaagggatca ttaaaataat aagaagaaaa
      721 tatgtcaaaa ggtaaaaaag taaattcttc tatccaaata tagaaaaatt gaatctagaa
      781 aaccgcccga caataaattt cattaactca gttgaccgaa aatgaagtag ataccaaaaa
      841 tttatcaaga gagcaagtag agaagtgagt atattcataa gagaaatagc tgtaacgaat
      901 tattaggtta atgcaaatgc ttatttatac tccgtataac tttctcagaa aaaatgatac
      961 caataatgac ttgctagtac tgtaaatccg tataatatca tctaatcaaa atggtgttcc
     1021 atttgcatct gtcatcgtaa tcgatccttc gggtgacact cgataaaaac caacgcttcc
     1081 agatcctcct tgggaacgga tttgtttgct ggaagctgtg aacaaataat ctcgatcaat
     1141 tttttgataa aagctaataa caatatcgtc actttgtaat tcggaacggt gggcaataag
     1201 aaatagaagt gctttgtcag agttttctaa tggaagagaa ggtacagtgc tttcttgcat
     1261 ttcagatgtt gaagaaatgc taaacgactg aatacttgat tggctatgtt gcgaatctac
     1321 attaccagta gaagtattag taatggaagt agtagaagta cttttagttg aaccaacctc
     1381 agttgttgaa atggctgctg aagattcttt aacagaattc tttgttttat atccacaggc
     1441 agaaaaaagt attacagttc ccaacaagcc tgcgtaaatc cctttatgca tgatagctcc
     1501 tcctaaaata gattatcttt attcagtata tcacttaaac aaagaaaagc agatggttta
     1561 aaatgaaaaa accttcccaa taaagggaag gttcatgaag ataataactt ttttaataat
     1621 tatagctttc taatatcttt catcaacttt tctagtaatc attgggaaaa atttcaagca
     1681 gtagatcgca caaataccta cgacgatata aatgatcttc gcaaaaatag ttgttgaacc
     1741 accagcaatc atagctacta aatcaagttc gaacaagcct accaacagcc agttcaaccc
     1801 tcccacaatc aataaagtta atgcaatagc atctaaagtt ttcattagaa ttctcctttc
     1861 ttaaatgata aaacaattat atatcaaaaa gagaaacatt gcttatataa cgctcaggaa
     1921 ttttagcaat ctatgcaaaa agatgaaaat tttttttgct cttccattag tacacatttt
     1981 ttagctgaag ctgtaaattt catgtcttaa ttttttttag tgagctttta tactggattt
     2041 gtaagaaaaa agaaggagag gatcacatat gtcggattgg ttggatattg caggaaaagt
     2101 agtaattgta actggaggtt cctcaggaat tggaagaagt atcgtcgaaa atttgttgaa
     2161 gcagaatgca caagtagcga attttgatgt aactgaatgc cgtatacagc atgaaaacct
     2221 actatcttta aaagtcgatg tgtcttctaa aacggacata gaagaaggga tatataaagt
     2281 aatgaaacat ttcaagacga tcgatggtct agtcaataat gcaggaataa atattccttc
     2341 tcttctaata gatagaaatc atccaaaaag caagtatgaa ttatcagaac aagtttttga
     2401 taagatgatc gctgtcaatc aaaaaagtgt atatttgatg agccaagcag ttggaagaat
     2461 cttggtgcaa aagggatcag gagtgatcgt gaatctttct tctgaatcag gattggaagg
     2521 atcagaagga cagagctgtt atgctgctac aaaagcagcg atgaacagtt ttactcgttc
     2581 ttgggcaaaa gagttaggaa agaggaatgt tcgtgtggta ggtgttgcac cagggatact
     2641 ggaagaaacc ggtttgagaa cacaagaata tgaagaagct ctttcttata cgagaggtat
     2701 aagcgtcgaa cagctgagaa acggttacag tcgaacaagt acgatccctc taggaaggag
     2761 tggtaagctg caagaagtgg cggatctagt ctgctattat ctttcagaaa gatcaagtta
     2821 tatcactgga gtcacgacta atatttcagg aggtaaaaca agaggataaa ggataggggg
     2881 gacgcacgtg acgctagtaa acagatggta ccaattattg aaattactag tcactcacaa
     2941 gaaagtttca atccaggaag tgcaaaaaga aatcaaaacc agtaatcaaa ccataaaaaa
     3001 agatattggg gagttgaatg aagaaattgc cggtattgca aaaatcatcc aaaaaaacaa
     3061 acattttgaa cttgaaattt atgattttga tttttttgat gagatcatgc aaggaaaatt
     3121 gaaaacagcc agtgatttca attcggcatc caaaagaatc gcttatttac taaataggct
     3181 gatcgaaaca gaggatttct tacgtatgga tgatttgtct gaagaagtcg gtgtcagcag
     3241 aggaacagtg gtcaaggatc taaagacatt aaaagagatg atcaatgatt ttgatgtaaa
     3301 gattacaggc acaccaaata aaggactaag aataatagga gatgagctag aattacgact
     3361 acttttattt tattttgttt ctccctactt ttcagagacg aaaacagaac agtttctaga
     3421 agaaaaacaa gcaatcaaac attttcagaa gaagacgaat gctagtaaac aagaaatcac
     3481 ccttttaacg aaagttatcg gtatttcttt gaatagaatc tctagtaact atctgttgaa
     3541 gaagcccatc aatcgttaca gtgggtgttt tgaatatatc caagaattca atgaactgat
     3601 caatcagttg gaagaaatct atgaactaac tttgagccaa tatgaaaaag attttatcag
     3661 ttttcctttg aacatcatca acacaagtat aacgatgata aggaaagcta actctgattt
     3721 gcgcttatat ttcgacccca tgatgatgcg tattcgacga ttagatctca ttgatctgga
     3781 tgagtccttc ttatatcaag agatgaaaca gcacctctct ttattgataa atcgggtaat
     3841 ttttcgttat cgattgactg atttatttta tggtgagata gaaaaaaagt atcctttttc
     3901 ttaccaaatc gctagcgaat gcatacaagc attagaagct atcttgcaac ataagatctc
     3961 taaagtagag atcagctacc taacgttgta tttggagtta gcatttcgat atcaaaaaga
     4021 gcatttttcc tctaaaaaag tagccattgt tcatacgaca ggaaaaggaa cagcattaat
     4081 gatccaaaga caaatcaaaa ggatattggg gagggatata caaataacac atgtttcaga
     4141 agaacaatat gaaaaagtag atctaaacaa atatttcgct gtttttacaa cgattccgtt
     4201 aaaaaatgta aatgaagaga caccggtgat ccatttgact aatctttttg atgactcttg
     4261 gttaagggca caatggcaaa aagcaaaaaa aaagcgatta gccgggtttc attctactgt
     4321 ttttttctat caaatatgga aagaaaaaaa gacgtatcaa gaatatttaa acaaattagt
     4381 cgctcctttg atcagaggct attatgtgga tgaagaattt gaaaaaagga tcttgaaaag
     4441 agaatatcag caaagcacat ttgttaacaa agaatttgct tttcctcaca caaccaacca
     4501 gctatctcaa cacattgttt tatcagtagg gctaataact gatcaaggtt tagcagaaac
     4561 aggaggaaat ggagttattt ttttatttgc catacctgaa gaaatgacgg ctgaagttga
     4621 gaatgaatta tttgagctat acgatatcct atttgaactc ataaaaatgg gaatagatga
     4681 gtttattgaa ttagcaccga cacaagaatc atttttaaaa ctaatcaaaa gtaaaggaga
     4741 gagacttcat gaatgatatg tatctattca tagttttcgc agcttcagct tatgtgctgc
     4801 aagcagttat cagttggaag caactaaagc atttcaatca aacatatagc gaactgcgaa
     4861 gaaaaggaaa agtagcgatt ggtaaacgac ctgggaaaat ccgttcaggc acgattgtta
     4921 tgctagctat agacgatcat gatcaagttt tagaagtcag aaaaatgcaa ggggttacat
     4981 ctttggcaaa atttcattca ttacctgtct atgcgggtga agatatccat tacatcgatc
     5041 gttaccatcc ctcagttcga gcggaagatc gtttgacaca agcagcttta gaaaatgcca
     5101 gagaagtata cttacgaata gaggcaggaa atgaaaaaag tgaacaacct tcattgcctt
     5161 ttattgctat gctcaataat tttcggatat ggaaaagtac gatcaaagaa aaaaggagtg
     5221 acaaacgatg gatatcataa caaaatttgc tgagggattt atgaggctgt tccaaacagg
     5281 tgcagaaaca ttcataagct ggatgacagg aatcgtacca gttgtcttga tgctgatggt
     5341 tgcaatgaat acgctgattg cttttttagg tgaagaccga gtaacgaaag tagccaaatt
     5401 atcagcgaaa aatccgctta ctcgttacct tgtgttaccg ttcttatctg cttttatgtt
     5461 gggaaatcct atgtcattta caatggctcg tttccttcca gaatactaca aaccaagcta
     5521 ttatgctgct caagctcaat tttgtcatac gagcaatggt gtattccccc atattaatcc
     5581 tggagaattg tttgtatgga tggggattgc tcaagggatc gaaaccttag ggctaaattc
     5641 gatggagctg gctatccgat acttgcttgt gggactgctg atgaacttta ttggtggttg
     5701 gataactgat tttactactg gatttgtatg cagacagcaa ggaatcgttc ttagcaaaaa
     5761 agtagagctt tcagttgatt agagaaagga gagttttaaa tgtatggtga tattaagatc
     5821 gtaagaggaa acggcgggta tggaggtcct ttgattatta ggccgacaaa agataaacac
     5881 aagttcatct atgtcacagg tggaggtgag aggccaatga tcgtagataa aatagaagaa
     5941 ctgacaggta tggagtctat caatgggttc aaaacatcaa tacctgatag tgaaatcgcg
     6001 cttgcaatcg ttgactgtgg agggactctt agatgtggaa tttatcctaa aaaagggata
     6061 ccaaccatca atatcgtttc cacaggaaaa agtggcccat tagcacaata tatcactgaa
     6121 gatatttatg tttcagcagt agggttagaa caaatacagc cactaatgga aactgatgat
     6181 aacaaagaaa cagtaaatac tgcatctcca gataaaaagt acaaatatga tacttctaaa
     6241 aagataaccg agcaaaaagc agagcagcaa aactggatag caaaaattgg aatgggcgcg
     6301 ggaaaagtag ttgctacatt caatcaatca gcaagagaag cagtagatac gatgatccgg
     6361 acagtgatcc cttttatggc tttcgtttcc cttttgattg gtgtgataca aggttctggg
     6421 atcggtaatg tatttgctaa cattatggca cctttagcag gaaatgcagt tggattaaca
     6481 atcattggct ttatttgttc actgccattt ttatctcctt tgcttggtcc aggggcagtt
     6541 attagccaag tgattggaac attgatcggt gtagagattg ggaaaggaaa tattcctccc
     6601 caattagcat taccggcact atttgctatc aatactcaaa atgcatgtga ttttattcca
     6661 gtcggtctag gtttggaaga agcaaaagct gagacagtag aagtaggtgt accatcagtt
     6721 ctttattcaa gatttcttaa cggcatccca cgagttatca ttgcttgggt agcaagtttt
     6781 ggattatata gctg
//
LOCUS       DAZKLD010000139         6763 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.140, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000139 DAZKLD010000000
VERSION     DAZKLD010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6763)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6763)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6763
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.140"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..887
                     /locus_tag="ACG8KR_002474"
     CDS             <1..887
                     /locus_tag="ACG8KR_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ArsR/SmtB family transcription factor"
                     /protein_id="HFQ0201791.1"
                     /translation="LQINEESLPVYEALASKTRIKIIQLLSKKKMNVKDLAKELGVSS
                     AITTMHVKKLEEANIIKTEKVGQQKISSLRVDKIDISFPEKIFNAFDTKETSIPIGHY
                     TNYAIEPTCGLATIHDFIGKVDEPRYFMDPRRMDARILWFTSGFVEYQAPNFLNTEDT
                     LEMLEVSVEISSEFPFSNDNWPSDITFSLNGVELGTWTSPGDFADIRGKYTPDWYPDN
                     LNQYGLLKTIRITKHLTNMNGEPLSNITINDIPKEQDTWHLRIEVKDDAKHVGGCTLF
                     GKGFGNYDQDIKLKVYYS"
     gene            complement(960..1784)
                     /gene="nadE"
                     /locus_tag="ACG8KR_002475"
     CDS             complement(960..1784)
                     /gene="nadE"
                     /locus_tag="ACG8KR_002475"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HFQ0201792.1"
                     /translation="MSLQTEIINELGVKPTIDPKEEIRKSIDFMKAYLKKYPFLKTFV
                     LGISGGQDSTLAGRLAQLTMEEMREETKDENYQFIAVRLPYGEQADEEDAKAALDFIQ
                     PDVSLRVNIKPAVDAQVQVLSEAGVGISDFNKGNIKARQRMITQYAVAGERAGAVLGT
                     DHAAENITGFFTKFGDGGADILPLFRLDKRQGKQLLQALNAPEKLYTKIPTADLEDGK
                     PMIADEVALGVTYNEIDDYLEGKEVPAQAQEKIEAWWNKTQHKRHLPISVLDDFWK"
     gene            complement(1796..3268)
                     /locus_tag="ACG8KR_002476"
     CDS             complement(1796..3268)
                     /locus_tag="ACG8KR_002476"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HFQ0201793.1"
                     /translation="MNNTYPDDSLTLHTDLYQINMMQTYWELGRADLHAVFECYFREM
                     PFNHGYAVFAGLERLVNYLENLTFNDSDIAYLRGLEVYPEGFLEYLQNFEFKATVRSA
                     REGELVFANEPLIQVEGPLAHCQLVETALLNMVNFQTLIATKAARIKSVIGEDPLLEF
                     GTRRAQELDAAVWGTRAAYIGGADATSNVRAGKIFGIPASGTHAHSLVQSYGNDYEAF
                     MAYAKTHKDCVFLVDTYDTLKSGVPSAIRVAKELGDKINFQGVRIDSGDMAYISKRVR
                     EQLDAAGFTEAKIYASNDLDEATILNLKMQKAKIDVWGVGTKLITAYDQPALGAVFKL
                     VSIEDDEGKMMDTIKLSSNAEKVTTPGKKQVWRITRNFDGKSEGDYVTLWDEDPREEE
                     AIFMFHPVHTFINKTVRDFTARPVLQDIFIEGKRVYELPALNEIKEYTKENLDSLWEE
                     YKRDLNPQKYPVDLSTECWNHKMAIMERMKKSVAELHTEA"
     gene            complement(3905..4267)
                     /locus_tag="ACG8KR_002477"
     CDS             complement(3905..4267)
                     /locus_tag="ACG8KR_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HFQ0201794.1"
                     /translation="MKLIETPVNSNLNIKTFYPKVVEFFFGNTAINYYKLFSLDRTQL
                     LLVDTYDKKQVVMINTKKKITRQEIDYAIHHVLKMTREDVKVHVGVKQELERAGIQFK
                     RPNKDIVVIEQKNTKDSI"
     gene            4490..5242
                     /locus_tag="ACG8KR_002478"
     CDS             4490..5242
                     /locus_tag="ACG8KR_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFQ0201795.1"
                     /translation="MEYTIKKMSEISGVSPRTLRFYDEIGLLKPARINSSGYRIYGKK
                     EVDRLQHILFYRTMAFKLDDIQEVLDNPSFDHQKALIKHQQMLLEKRAQIDTLLTTVQ
                     QTLDMYEGGRKMSDKEKFEGFKQQKLKENEESYGQEIRQKYGKKKVTASNEKWMNMDK
                     ETYEEMQTVEKQMLFDLTLYLKEPEDESLADRIFQAHKKWFSYSWPEYQPKAHKGLGM
                     MYEADERFTAYYDERSGHGAAKALNEIIQKKA"
     gene            complement(5382..6305)
                     /locus_tag="ACG8KR_002479"
     CDS             complement(5382..6305)
                     /locus_tag="ACG8KR_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0201796.1"
                     /translation="MANIRDIAKLTGYSVSTISRVINGHPYVDEEKRKEILAVMKEVD
                     YIPNANARQLSYGKTKSIGVILPYTNHPYFDQLISGIIEAAFDYDYKVTLLPTGYHKE
                     KERRYLEEFAAKAFDGLIITSRANTLDDLLAYQKYGPLVFCEEIKGKQASCVFIDREQ
                     SITEGLTYLKQQGIKQLGITLGRSGRLSYNSKITLQLCHQLFPSFDESLVFWDCIDAE
                     KGKQAGFFFKELGVDGVFTNSDMVAAGILQSYLQDKTPVVIGRDNLLISELLGFPTID
                     HHLEACGEMAFQLFLTEKKDKVKIPYTFIQR"
     gene            complement(6320..6592)
                     /locus_tag="ACG8KR_002480"
     CDS             complement(6320..6592)
                     /locus_tag="ACG8KR_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0201797.1"
                     /translation="MNTYVEITDGTTNNYFYAFVEFKGSVLTLYSFQGLNRNIVKEIP
                     MNEIENLTKDIYWGGQRISFSHEGKMYQFFECGPAVVDYLQENLFV"
BASE COUNT         2064 a         1349 c         1155 g         2195 t
ORIGIN      
        1 aactacaaat aaacgaagaa tccttaccgg tatatgaagc attagctagt aaaacaagga
       61 tcaagatcat ccagctactt tccaaaaaaa agatgaatgt caaagatttg gcaaaagaac
      121 ttggagtgag cagtgctatc acgacgatgc acgtgaaaaa gctagaggaa gcaaacatca
      181 tcaaaacaga aaaagtagga caacaaaaaa tttcgagcct tcgtgtcgat aaaatcgata
      241 tcagttttcc agaaaaaata ttcaatgctt ttgatacgaa agaaacatcg atacctattg
      301 gtcactatac aaattatgca attgaaccaa catgcggttt agctacgatc catgatttta
      361 tcgggaaagt ggatgaacca cgctatttta tggatcctcg aaggatggat gcccgcattc
      421 tctggtttac cagcggattt gtcgagtatc aggcacctaa ttttttaaat acagaggata
      481 cattggagat gttggaagtt tctgtggaaa tttcttctga atttcctttt tcaaatgaca
      541 attggccttc agatatcact ttcagcctaa atggtgtgga gctaggaaca tggacgagcc
      601 ctggagattt tgctgatatc cgcggtaaat atacgcctga ttggtatcca gataatctta
      661 atcaatacgg cttgctgaag acgatccgaa ttaccaaaca cttgacgaat atgaatggag
      721 aacccttgtc aaacatcacg atcaacgaca tccctaaaga gcaagacact tggcatttgc
      781 gaatcgaagt gaaagacgat gccaaacatg tggggggctg tactttattt ggtaaaggat
      841 tcggtaatta cgatcaggat atcaaattaa aagtttacta cagctgatat ttttcttttc
      901 tattttataa aacaaaaaca gccaatccct atgaagggac ggctgttttt gctttattgt
      961 tatttccaga aatcatccaa aacggaaatt ggcaaatgtc gtttatgttg tgtcttgttc
     1021 caccatgctt cgattttttc ttgtgcttga gcgggaactt cttttccttc tagataatca
     1081 tcaatttcat tatatgtgac acctagtgcg acttcatctg caatcattgg tttcccatct
     1141 tcaagatctg cagtcggaat ttttgtgtat aatttttccg gtgcattcaa tgcttgaagc
     1201 aattgtttcc cttgtcgttt gtccaaacgg aataatggca aaatgtcggc tccaccatca
     1261 ccaaatttcg tgaagaatcc agtaatgttt tctgcagcat gatctgtccc taatacggct
     1321 cctgctcttt ctccagctac ggcatattgc gtaatcatac gttgacgtgc tttgatattt
     1381 cctttgttaa aatcgctgat tcccacacct gcttcgctta atacttgtac ttgtgcatca
     1441 acagctggtt tgatatttac tcgcagacta acatcgggct ggataaagtc aagagcagct
     1501 tttgcatctt cttcatctgc ttgttcacca taaggaagac gaacagcgat aaattgatag
     1561 ttttcatctt ttgtttcttc acgcatttct tccatcgtta gttgcgccaa acggcctgct
     1621 aaagtagaat cttggccgcc gctgatacct agtacaaaag ttttaagaaa cggatatttt
     1681 ttcaaatatg ctttcataaa gtcaatactt ttacgtattt cttcttttgg atcaattgtt
     1741 ggtttgacac ctaattcatt gataatttct gtctgcaggc tcatttattc tcctcctatg
     1801 cttctgtatg aagttctgct acgctttttt tcattcgttc catgatagcc attttatggt
     1861 tccagcattc agtagacaga tcgactgggt atttttgcgg attcaagtct cgtttatact
     1921 cttcccatag tgaatctaga ttttcttttg tatattcttt gatttcgttt agtgcaggta
     1981 attcgtaaac tctttttcct tcgataaaga tatcttgtaa aactgggcga gctgtaaagt
     2041 ctcgaacagt tttattgata aacgtatgca ctggatggaa catgaagata gcttcttctt
     2101 ctcgagggtc ttcatcccat aatgtcacgt agtctccttc agatttccca tcaaaatttc
     2161 gagtgatgcg ccacacttgt tttttccctg gagtagttac tttttctgca ttgcttgata
     2221 acttgatcgt atccatcatt tttccttcat catcttcgat cgatactagc ttgaatactg
     2281 caccaagggc tggctgatca taggctgtga tcaattttgt cccaacaccc catacatcaa
     2341 ttttagcttt ttgcatcttt aaattcaaaa tcgttgcttc atccaagtca ttcgaagcat
     2401 agatttttgc ttccgtaaat ccggctgcat ctaattgttc ccgaacacgc ttagatatat
     2461 aggccatatc tccactgtcg atgcgtacgc cttggaagtt gattttgtca cctaattctt
     2521 tcgccacgcg aatcgcactt ggcacacctg atttcaacgt atcataagtg tcgactaaga
     2581 acacgcagtc tttatgggtt ttagcgtagg ccataaatgc ttcataatca tttccataag
     2641 attggactaa cgaatgagca tgggtcccgc tagctggtat cccaaatatc ttcccagcac
     2701 gtacattgct tgttgcatct gctccgccga tatatgccgc tctagttccc cagactgctg
     2761 catccagttc ttgtgcccgt ctagttccaa attccaatag tggatcttct ccgatgacag
     2821 atttgatacg agctgctttt gtcgcgatca acgtttggaa attgaccata ttcaacagtg
     2881 ctgtttctac taattggcag tgcgctaaag gcccttccac ttggattaat ggttcatttg
     2941 caaaaactag ttctccttca cgagcagaac gaactgttgc tttaaattca aaattttgta
     3001 aatactccaa aaaaccttca ggatagactt ctaatccacg caagtaagca atatcggaat
     3061 cattgaacgt cagattctct aaataattga ctaaccgttc caatcctgca aaaaccgcgt
     3121 atccgtggtt aaaaggcatt tctcgaaagt aacattcaaa aacagcatgt aaatcagctc
     3181 gacctaattc ccagtatgtc tgcatcatat tgatttgata tagatccgta tgcagcgtca
     3241 aactgtcgtc tgggtaagta ttattcatct tgtattactc ctcttggcaa gttttcattt
     3301 cctacccatt ataacaaaaa aaattaatag gaaaagaaat cttccatttt ttctgaaaaa
     3361 gcacatgttt tacggatatt tcttgctaaa aatgaactcc atctgctaaa actatccttt
     3421 cttattggta tttaacctaa acgagaaaga atacttattt tattcaatgt atccgagaca
     3481 cgaagaaatt agcgttttct atttttctta cttgataaaa taagtagatt ttcctactac
     3541 aatcgtagcc gttaagatac cgatgattga acaaatataa ccaaatgtct ctacaacata
     3601 atcaatggca aaagtttttg aagcaataat acgtctttcc ttttttgctt cttatcccca
     3661 aagaaacgga tataacttat tatgtattga atttttttgg aaaggcgaat tatgacaaga
     3721 tataccttgc gttgaaaaaa ctcgcggaaa aaatatcaca cgtttttaaa aagatacaaa
     3781 gtaagaaagc agctgtcaat agaatggaaa caaaaatggc gagacatcaa tcagtctcgc
     3841 cttgtttttt attgtataca ttagatatgg agcaggttac cataaagatg attattagtc
     3901 gttattaaat agaatccttc gtattttttt gttctatcac tacaatatct ttatttggac
     3961 gtttgaattg tattccggca cgttctagct cctgtttcac tcctacatgg actttgacat
     4021 cttctcgtgt catttttaaa acgtgatgaa tcgcatagtc gatttcttgc cttgtgattt
     4081 ttttcttcgt attgatcatc acgacttgct ttttatcata tgtgtctacc aatagcagtt
     4141 gtgttcgatc taacgaaaat aatttgtaat aattaatagc cgtattccca aaaaagaatt
     4201 ccactacttt aggataaaat gtcttaatat ttaaattact attcacaggt gtttcgatta
     4261 gcttcatctc attcctcttt tctatatagt cgatttagat ccaatcttaa gttatgttac
     4321 catctttcct agcattaagg aatcggttac aattgaacct ttaaagacaa aaggatttac
     4381 cttttatcac ttaatagtat aacataattt cgtgagaaaa aaatacaatt ttttcttgac
     4441 catctcgtta cgtaaaggat tatgcttaca tttgtcagga ggaaaaataa tggagtacac
     4501 aataaagaaa atgtcagaga tatcaggagt cagtccgaga accttgcgct tttatgatga
     4561 gattggactg ctaaaaccag ctaggatcaa ttcttcaggt tatcggattt atggcaaaaa
     4621 agaagtcgat cgtctgcaac acatcttatt ttatcgaaca atggcattca agttggatga
     4681 tatccaagag gtcttagaca atccatcttt tgaccaccag aaagctttaa tcaagcatca
     4741 gcaaatgttg ctggaaaaac gggcacaaat cgataccctc ttgacaactg tccaacagac
     4801 acttgatatg tacgaaggag gaagaaaaat gagtgacaaa gaaaaatttg aagggtttaa
     4861 acaacaaaag ttgaaagaaa atgaagaatc ctatggacaa gagattcgac aaaaatacgg
     4921 aaaaaaaaag gttacagcat ccaatgaaaa atggatgaat atggataaag aaacgtatga
     4981 agaaatgcaa actgttgaaa agcagatgtt atttgatctc actctttact taaaagaacc
     5041 agaagatgaa agcttagcag accgtatctt ccaagctcat aagaagtggt tctcttacag
     5101 ctggcctgaa tatcagccaa aagcgcataa aggactagga atgatgtacg aagccgatga
     5161 acggttcact gcatattatg acgagcgaag tggtcatggt gcagcaaaag ctttaaatga
     5221 aattatccaa aaaaaggcgt aatcttttac atgatccaac aaaaaagtac acgtaaaatg
     5281 acctaataac aaaacagcag acacccaagt tagattttta tgctaacttt tggtgtctgc
     5341 tccattcttt cacaagtttt ttcatcctac tttaaatata ttcatcgttg aataaacgta
     5401 taaggtattt tcactttatc ctttttttct gtaagaaaca attgaaatgc catttctccg
     5461 caagcttcca gatggtggtc gatggttggg aaaccaagaa gctcgctgat cagcaaatta
     5521 tcgcgtccaa tcaccactgg tgtcttatct tgcagatagc tttgcaagat gcctgctgca
     5581 accatatccg aatttgtaaa gacaccatca actccaagct ctttaaaaaa gaagcctgct
     5641 tgttttcctt tctctgcatc tatacaatcc caaaaaacaa gtgattcatc aaaagatgga
     5701 aaaagttgat gacatagctg caaagtgatt tttgagttat aacttagcct gccacttcgc
     5761 cccaacgtga tgcccaattg ctttatacct tgctgcttca aataagtaag accttctgta
     5821 attgattgtt ctcgatcaat aaagacacag cttgcctgtt ttcccttgat ttcttcacaa
     5881 aaaactagag gtccatattt ttgatatgcc aataaatcat ctagtgtatt ggcacgagac
     5941 gtaatgatca atccatcgaa agctttagct gcaaattctt caagatacct tcgctctttt
     6001 tctttatgat agcccgtcgg taaaagtgtc actttgtaat cataatcaaa cgctgcttca
     6061 atgatcccac tgatcaactg atcaaaatac ggatgattgg tataaggcag aatcactcca
     6121 atgcttttcg tttttccata acttaactgt ctggcatttg cattagggat ataatcgact
     6181 tctttcatga ccgcaagtat ttctttcctt ttttcctcat ctacatatgg atgtccattg
     6241 attacacgtg aaatagtaga aactgaataa cctgtgagct ttgcgatatc tcgaatgttt
     6301 gccactgctg cttcgccacc tatacaaaaa gattttcttg taaatagtct accacagctg
     6361 gaccgcattc aaaaaactga tacatttttc cttcatgcga aaaactgatc cgctgcccgc
     6421 cccaatatat atcttttgtc aaattttcga tttcattcat tggtatttct tttactatat
     6481 tcctattcaa cccttgaaaa ctgtacaacg ttaatacgga tcccttgaac tcgacaaacg
     6541 cataaaaata attgttcgtt gtgccatctg ttatctctac ataagtattc attgcaagcc
     6601 ctccttttga tactttgagt atagtgtatg aaacgcgtct catagcaagg agtttgtttt
     6661 gccaaagaaa agttttattc ctctttcatt attaccaaag tctcttattt taaattgaat
     6721 acattataat gatactgtaa atatatcttt attattagtg aat
//
LOCUS       DAZKLD010000140         6572 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.141, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000140 DAZKLD010000000
VERSION     DAZKLD010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6572
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.141"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            242..931
                     /locus_tag="ACG8KR_002481"
     CDS             242..931
                     /locus_tag="ACG8KR_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFQ0201798.1"
                     /translation="MKILIADDDKEIVELLSIYVHNEGYEAVKAYDGKEALSKIRTIP
                     DIELLILDIMMPEMDGMQVVKELRKESQIPIIMLTAKTTDMDKIKGLVAGADDYVTKP
                     FNPLEVMARVKSLLRRTKMQIAPDQPDVLEVNALIINKDSHEVKTIDGKEIQLTALEF
                     GILYLLASHPNRVFSADEIFERVWKQESIVSAKTVMVHVSHLRDKIEEATGGEKVIQT
                     VWGVGYKIDAR"
     gene            918..2126
                     /locus_tag="ACG8KR_002482"
     CDS             918..2126
                     /locus_tag="ACG8KR_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HFQ0201799.1"
                     /translation="MTQDSKRATEKRRRINLTSKEISELLAEGIITIILLLLLNVSIL
                     VVISSVINSSPSLTNAIWDSKNIFAERLNTDLFWNGRNFIIPFFFLLDIGVLYWRLIR
                     RYRQMQLRHIISELHYIANGNYDHRIPFELSGDLSRVVTSINGLVDSTVAAIEDERKI
                     EKSKDELITNVSHDIRTPLTSIIGYLGLIEDGQYHSEEDLLKYTHTAYIKAKQMKSLV
                     DDLFEYTKVRQPAVPVNFSAFDMIQLIEQLAADFELEASKKNIQILVQSKVDSLIMDG
                     DTEKLVRVFNNLLTNALKYGKGATKIVIEVERIGSEVVATVKNNGAMIPQQAIDNLFD
                     RFYRVEESRSQATGGTGLGLAIAQSIVALHGGYIYAKSDKQWTSFIMHLPIKKNEKLP
                     IDTQEVIDES"
     gene            2420..3691
                     /gene="serS"
                     /locus_tag="ACG8KR_002483"
     CDS             2420..3691
                     /gene="serS"
                     /locus_tag="ACG8KR_002483"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HFQ0201800.1"
                     /translation="MLDVKMMRQNIEEVQEKLMTRGVKKETLAEFMELDENRRKLLVK
                     SEELKKYRNDVSAEIAQLKRAKEDASAKIAEMKEVGGNIKALDTEIAEVDEKLKQIAT
                     TLPNLPHDSVPVGTDEEDNVEIRRWSEPRTFAFEPKPHWEIAENLDILDFERGAKVAG
                     SRFVYYKGLGARLERAVYNFMLDQHIYEHGYTEMITPYIVNSKAMFGTGQFPKFKEDV
                     FQLENTDLTLIPTAEVPLTNYYNDEILDGADLPIYFTALSPAFRSEAGSAGRDTRGLI
                     RLHQFNKVEMVKFSDVEHSYEELEKMTADAEDILKKLHLPYRVMALSTGDMGFSAAKT
                     YDLEVWIPAQDTYREISSCSNCEDFQARRAMIRYRDNDGKIHYTHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTVPEALVPYMGNLKVIK"
     gene            complement(4240..5724)
                     /gene="guaB"
                     /locus_tag="ACG8KR_002484"
     CDS             complement(4240..5724)
                     /gene="guaB"
                     /locus_tag="ACG8KR_002484"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HFQ0201801.1"
                     /translation="MSNWETKFTKKGFTFDDVLLIPAESHVLPNEVDMSVQLAKNIKL
                     NIPIISASMDTVTDSKMAIAMARQGGLGVIHKNMTISQQADEVRKVKRSESGVIIDPF
                     FLTPQHLVADAEELMSKYRISGVPIVETLENRKLVGIITNRDMRFVTDYHMPIADVMT
                     KDNLVTAPVGTSLKDAEKILQKHKIEKLPIVDNEGRLSGLITIKDIEKVIEFPNAAKD
                     EHGRLLVAAAVGVTSDTFERANALLEAGADAIIIDTAHGHSAGVIRKIQEIRSTFADA
                     TLIAGNVATAEATKALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQLTAIYDAASVAR
                     QYGKAIIADGGIKYSGDIVKALAAGGHAVMLGSMLAGTDESPGEFEIFQGRRFKTYRG
                     MGSLGAMEKGSKDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIIFQMIGGLRSGMGY
                     VGAANLQQLRDEAQFIQMSGSGLKESHPHDVQITKEAPNYSVES"
     gene            complement(6229..>6572)
                     /locus_tag="ACG8KR_002485"
     CDS             complement(6229..>6572)
                     /locus_tag="ACG8KR_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1129 family protein"
                     /protein_id="HFQ0201802.1"
                     /translation="SRGTTPAYGFSTLVLGSAIGGWVFYMMYKYIYQYEYPGADRSKK
                     PGMGKTLLILGGTTLLWVVLFSATTLLPPVINPVLDPVVVIIIGATALAIRYYLKKKY
                     GIIGSLSVPRR"
BASE COUNT         2006 a         1248 c         1287 g         2031 t
ORIGIN      
        1 atttatcaac atttctagct tacatgatca ttggttagtt ctggtccttt ttcgttcgtc
       61 tttataactc cgagagacgt aaaatccttt tgttttcagt ttaatgttca tgttcttgtc
      121 ttattcacac caactagatg tctcaccttc taaaattttc cttaacaaag tgctgctttt
      181 atctatttct gctttttttt gctagaataa tagtaacaat atgactctgg aggatttatt
      241 tatgaaaatt ttgattgctg acgatgataa agaaatcgtt gaattattaa gtatctatgt
      301 tcacaatgag gggtatgaag cagtaaaggc ttatgatggg aaagaagcat tatctaaaat
      361 cagaacaatc cctgatattg aattacttat tttagatatc atgatgcctg agatggatgg
      421 gatgcaagta gtgaaagagc tacgaaaaga atcccaaata ccaatcatta tgctgacagc
      481 aaaaactact gatatggata agatcaaagg actggtcgct ggtgcagatg attatgtgac
      541 caagccattt aatccgttag aagtaatggc tagagtaaag tctttattac ggcgtacaaa
      601 aatgcagatt gccccagatc agcctgatgt attagaagta aatgcattaa tcatcaataa
      661 agactctcat gaagttaaaa caatcgatgg gaaagaaatc cagttaacag ctttggaatt
      721 cggtatcctc tacttgcttg ctagtcatcc taatcgagta tttagcgcgg atgaaatttt
      781 tgagcgtgtt tggaaacaag aaagtattgt ctctgctaaa actgtcatgg ttcacgttag
      841 tcatttaaga gataagattg aagaagcaac tggtggtgaa aaagtcattc aaaccgtttg
      901 gggagtgggc tataaaattg acgcaagata gtaaacgagc aactgaaaaa cgccgtcgga
      961 tcaatttgac ttcaaaagaa atcagcgaat tgttagcgga aggaatcatc acgattatcc
     1021 ttttgcttct attaaatgtt tctattctgg ttgttatcag ttcggtgatc aacagttcac
     1081 cttctttgac gaatgcgatt tgggattcaa agaatatttt tgcagaacga ttgaacactg
     1141 atttgttctg gaacggccgg aattttatta ttcccttttt ctttctatta gatattggtg
     1201 ttttgtattg gcgcttgatt cgaagatatc gccaaatgca attgcggcat atcatcagcg
     1261 aacttcacta catcgcaaac ggaaattatg atcatcgtat cccttttgaa ctgagtggtg
     1321 acttaagccg agtggtaaca agtatcaatg gattggtgga tagtaccgta gcagcgattg
     1381 aagacgaacg taaaatcgaa aaatcaaaag atgaattgat cacgaatgtc agtcatgata
     1441 tccgtacccc actcacgtcc atcattggat acctaggact gatagaagat ggccagtatc
     1501 actcagaaga agatctttta aagtatacgc atactgctta tatcaaagca aagcagatga
     1561 aatctttagt ggacgactta tttgaataca ctaaggtacg ccagcctgct gtcccggtga
     1621 atttttctgc ctttgacatg atccagttga tcgaacagtt agcagctgat tttgagttgg
     1681 aagcttctaa aaaaaatatc caaatacttg ttcaatcaaa agtagactcc cttatcatgg
     1741 atggcgatac cgaaaaactt gtacgtgtct tcaataatct tttgacgaat gcgttgaaat
     1801 acggaaaagg agcaacgaag atcgtaatcg aagtagaacg tatcggttca gaagttgttg
     1861 caacagtcaa aaataacgga gcaatgatcc cacaacaggc aatcgataat ctattcgacc
     1921 gtttttatcg tgtagaagaa tctcgttcac aagcaactgg aggaactgga cttggtttag
     1981 caatcgctca aagcattgtg gctttgcacg gcggatacat ttatgcaaaa tcagataagc
     2041 aatggacttc tttcatcatg caccttccaa tcaagaaaaa tgaaaaatta cctattgata
     2101 cacaggaagt tattgacgaa agctgaaata ttcgctattc ttattacaat tattagaata
     2161 aatgaaaaac acagaaccca gtacacttta ttctttttca gagagctgat ggcaggtgta
     2221 aatcagcaaa ggataatttg gaagttccag ttcgtttacg tgccctttcg aaataagaaa
     2281 ggtcggttca acagccgtta tgtgttcaac aagtgcagct gacgctgaac ttgggtggta
     2341 ccgcgaattt cagactgatt cgtcccaaga ataatgggat ggatcagtct ttttttattt
     2401 tcaaaaagga ggaaatcata tgttagatgt aaaaatgatg cgacaaaata ttgaagaagt
     2461 acaagaaaaa ttgatgactc ggggcgtaaa aaaagaaaca ttagctgaat tcatggaact
     2521 agatgaaaat cgtcgtaagc tactagtcaa aagtgaagaa ttaaaaaaat atcgaaatga
     2581 tgtttctgct gagatcgccc aattgaagcg tgcaaaagaa gatgcttctg ctaaaatcgc
     2641 tgaaatgaaa gaagtcggtg gcaacatcaa agcattggat actgaaattg ctgaagtgga
     2701 tgaaaaattg aaacaaattg caactacttt gccaaacctt ccccatgatt ctgtgccagt
     2761 tggaacagat gaagaggaca atgtagaaat ccgtcgttgg agcgaaccac gtacatttgc
     2821 ttttgaacca aaaccacact gggaaattgc agaaaatctt gatattttag attttgaacg
     2881 tggcgcaaaa gtagccggta gccgttttgt ctattacaaa ggattaggtg ctcgtttaga
     2941 acgtgctgtc tacaacttca tgcttgatca gcatatttac gaacacggct atactgaaat
     3001 gatcactcct tatatcgtca acagtaaagc gatgtttggt acgggacaat ttccaaaatt
     3061 caaagaagat gttttccaat tggagaatac tgatctaaca ttaatcccaa cagctgaagt
     3121 accattaact aactattata atgatgaaat tttagatggt gcagatttac ctatttattt
     3181 cactgcacta agccctgctt ttcgttctga agccggaagt gctggtcggg atacacgagg
     3241 actgattcgt cttcaccaat tcaataaagt agaaatggtc aaattttcgg atgtagagca
     3301 ttcttacgaa gaattggaaa aaatgacagc tgatgcagaa gatatcttga aaaaattgca
     3361 tttgccttat cgtgtgatgg ccttatcaac tggagacatg ggcttttctg cagctaaaac
     3421 ttatgactta gaagtatgga tacctgcaca agatacttat cgtgaaatca gttcttgttc
     3481 taactgtgaa gatttccaag cacgccgcgc aatgatccgt tatcgcgata atgatggaaa
     3541 aatccactat actcatactc tgaatggttc aggacttgca gttggccgta cagtagctgc
     3601 cattttggaa aactaccaaa atgaagatgg ttctgtcaca gttccagaag cgctcgttcc
     3661 ttatatgggg aatctgaaag ttattaaata aagaggctgt gaaaaagccg cttagtttcg
     3721 agtattaaga aaaattttgt gaaaatggct ttccacattt ttgcataaat tggacttaat
     3781 accgagaaac tggcttttga acaccattta tcgagaggtt gtgaaatcag cgaattaacc
     3841 tgagtattag agaaaaaaat ggaaaaatag tccctcatat tttaccgttt gttgaactaa
     3901 taccgagggt cagctgattg aacaccgttt agtggagagg tcgtaagcct accccccagc
     3961 tcctagttac aaggacaaat tcaaaaaaca gctcttcata ttttatgaat gttgtacttg
     4021 tgattcggag gtggcaaggg aacactgttt attcaaaaag aggctgggac agaagcgttt
     4081 aactccaaga aataagaaga aattcacgaa aattgctttt caaatttttg tgaatttctt
     4141 cttatttccg aaggagttgc ttctacttcc gccgtttata cgtgtttaga gcgtgagaca
     4201 aaagtgttct ttacttttgt cccacgctct ttaatgtttt taagattcaa ctgaatagtt
     4261 tggtgcttct tttgtgattt gtacatcatg agggtgtgat tcttttaatc cactgccgct
     4321 catttggata aattgcgctt catcacgtaa ttgctgtaga ttggctgccc caacataacc
     4381 cattcctgaa cgcaaaccgc cgatcatttg gaaaataata tctgcaacac ttcctttata
     4441 agcaacccga ccttcgattc cttctggtac taatttattt gcttcattga cactaccttg
     4501 gaagtaacgg tcttttgaac ctttttccat cgcgcctaat gagcccatac ctcggtatgt
     4561 tttgaatcga cgtccttgga agatttcgaa ttcacctggt gattcatctg tccctgccaa
     4621 catacttcct agcatcactg catgtccgcc ggcagctaat gctttgacga tgtcaccaga
     4681 atatttgatc cctccgtctg caataatcgc tttgccatat tgacgagcaa ctgatgctgc
     4741 atcataaata gctgtgagct gaggtacacc tacaccggct accacacgtg tcgtacaaat
     4801 cgatcctggt ccgataccaa cttttacgac atctacacct gcatcatata aagcttttgt
     4861 cgcttcagcc gtcgcaacgt ttcctgcaat caatgttgca tctgcaaaag ttgaacggat
     4921 ttcttgaatt tttcggatca cacctgcact atgtccatga gctgtatcga tgatgattgc
     4981 atctgcccct gcttctaata aagcattcgc acgctcaaat gtatcgcttg ttacaccaac
     5041 ggctgctgct acaagcaatc gaccatgttc atcttttgct gcatttggaa attcgatcac
     5101 tttctcgatg tctttgatcg tgatcaatcc gcttaaacga ccttcgttat ctacgattgg
     5161 taatttttcg attttatgtt tttgcaaaat cttttctgca tcttttagag aggtacctac
     5221 tggggcagtc actaagttgt cttttgtcat cacatcagca atcggcatgt gataatctgt
     5281 tacaaaacgc atgtcgcggt ttgtaattat ccctaccaat tttctgtttt caagtgtttc
     5341 aacgattggc acaccactga tacgatattt gctcatcaat tcttctgcgt ctgctacaag
     5401 atgctgtggc gttaagaaga acggatcaat gattacgcca ctttcagaac gtttgacttt
     5461 tcttacttca tccgcttgct ggctgatcgt catgttttta tggatcacac ctagtccgcc
     5521 ttgtcttgcc atagcaatgg ccatcttgct gtcggtaaca gtatccatac ttgcactgat
     5581 gattggaata ttcaacttaa tatttttagc taattgtacg ctcatatcca cttcgtttgg
     5641 taacacgtga ctttctgctg gtatcaataa gacatcatca aaagtaaatc cttttttcgt
     5701 gaactttgtt tcccagttgg acatttttag taccactcct ctaatttagt ttatctatta
     5761 aaataattga ttttttcatt taagtcaatg atctacttgg aatcaagtta gctttctcat
     5821 agaataccaa tataaagcgt ttacatctgt gtgtattaaa agagattgta aaaaagctgt
     5881 cctgcatcaa gtaataagaa cagccaatca aaaacagctt ctcatgtttt tgaattggtt
     5941 gggtttatta ccgcagatgc aaacttttga acaccgttta gtggagaggc tgtgaaaaag
     6001 ctgcttagtt tcgagtagta agaaaaattt tgtgaaaatg gctttccaca tttttgcata
     6061 aattggactt aataccgaga aactggcttt tgaacaccgt ttatcgtaaa aagggactgt
     6121 gacaagactt ttgtcacagt cccttactat tatttaacta tggataaaca aaatgaggtt
     6181 ctttactacc cctagatatc tagcaaaata gctgatcttt ttgctagttt atcttcttgg
     6241 gacggataaa cttccaataa tcccgtattt tttcttcaag tagtaacgga ttgctaaagc
     6301 agttgccccg ataatgataa ctactactgg atctaatact ggattgataa ctggtggcaa
     6361 tagtgttgtc gcagaaaaca gtacgaccca aagaagtgtc gttccaccta ggataagcaa
     6421 tgtcttaccc attcctggtt tcttgcttcg atctgctccg ggatattcat attgatagat
     6481 gtatttgtac atcatataaa agacccagcc accgattgca gaacctagaa cgagtgtaga
     6541 aaagccataa gctggcgttg ttccacgtga aa
//
LOCUS       DAZKLD010000141         6386 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.142, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000141 DAZKLD010000000
VERSION     DAZKLD010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6386
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.142"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            149..2824
                     /locus_tag="ACG8KR_002486"
     CDS             149..2824
                     /locus_tag="ACG8KR_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF927 domain-containing protein"
                     /protein_id="HFQ0201803.1"
                     /translation="MKMNHTSIQKHLQLLNTLGISYVNLRTFSTLNKKTVSHIFSTAD
                     SQAIINYLEKQKLSEGTVNITYNPIKQEVITQKKHSVRDNDIEKIRFVFIDIDPKRKE
                     GSATDSEKKKAENVMEQVERYLKEKRIESFVKVDSGNGYHIFLPINEQPNNHETILTI
                     QNFLQLLHRRFGVEDEVDIDTSVYNPSRLCKLVGTPAVKGKSTKERPHRLSSFLSIPD
                     NIDRSNCFKILEQIVEENSMMSTSRVKSHPSFREVLPFIQADYKQWLAHYPELTYTIK
                     EGDHKGVTLLIFDECPLRKHTNNSNGSCLSVTADNKIRFHCLHASDKNKDIQDFVKKY
                     PLPKPVNPSVESLESNQEYYFDQFKLNQEGVFLVKEGQEEKVACPMFIKQIRKNIEQK
                     VITLVLAYYVDHCWDEIEIEGGCLQTNNFKQLAKHGLIIKARREAEVVDFLQIQRENA
                     PIFFEHESLGWKKENEAMIFQLQDSFGREIPSVLTKNSCYQLTTNGTEAEFDALVHKY
                     VLGTNLELAWSIGIASIVLGYLNVTKANTFLSLLINLQGKSTTGKTTTLHFIASLFGN
                     PTTVLRNMNATNKAIIKLASNNQGGVPLILDELGAATQNNFSSLIYQLASGEERLRLD
                     SNCQLMPQETFSVLTLTSSEERLENYFNEKLTGLKVRYIEFSDIKFTKDANHAEKVKS
                     LSSNTFGWGIKLFLLKLFEQGITKIEATFVEAQKKLWAVLPEHSLKQRIGNNLALIYS
                     GALLIQDILGYPLRLEHIQTVLINSYEQLIVDTSLGSEQTVFDRVKTLLVMNSHKFLT
                     NSDNKHTPSTIWGKVSIQKNGDIKVNIFPIPFEDLLRREFQVKDLTYLFRQLLDSGNM
                     CAEKGRRTKRIHMNRQVLPTYEILLPSSLKSYFRL"
     gene            3006..3245
                     /locus_tag="ACG8KR_002487"
     CDS             3006..3245
                     /locus_tag="ACG8KR_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0201804.1"
                     /translation="MTKNKMTLKAEVLLYIQEHFSNQAFFTKPIYLDFEIRGVSAGSI
                     GGTLQALKNEGYLENHFVQRSFNRRVVKKWYLVHS"
     gene            3261..4634
                     /locus_tag="ACG8KR_002488"
     CDS             3261..4634
                     /locus_tag="ACG8KR_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFQ0201805.1"
                     /translation="MRVACYDRTSVYEEAKISNDAQYDAVKEYVLEKYEQIPQRFSDK
                     GESGEKAERPAFRQLRAWVCASEEEGVIYVWRYDRLARQAKIALEFIELCQQHQVQVY
                     SISEPLPASFSHASTNKLFVQLLFTFAEQQRNVIIENVRAGLSYKKQQRQYLSSAVPY
                     GYRLVNGKIQQEVHEARIVRYIFQLYLTKKYGYKKLCQRLTQQKLFFRERPFQPYHIY
                     SILKNPLYYGEIKGGSLGKYLGTFEPILSKATFLQAQEIRQSRCTAKKDTYLYLLRQK
                     IRCPFCGRHLSSKYQWNTKKTKTLHYYHCTDRSCQGIFLRAERVESEVLEKIQAFFQY
                     EGVYQALIAQLTQQLAEKRKDEKRKVIQQKKQEAQWIKAFEEGNCSLEELKQQLQALQ
                     KPMSTSSFLSLQQCEAKVKEWLQLKDLSSRQLLCDYVCRIEVTKDKTVCGIYFRQLTQ
                     NILERNE"
     gene            4638..6272
                     /locus_tag="ACG8KR_002489"
     CDS             4638..6272
                     /locus_tag="ACG8KR_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFQ0201806.1"
                     /translation="MEMIQTKRVALYIRVSTQEQALEGHSIQAQEQNGRQFAERMGYE
                     VVEVYADEGISGKSTKNRFAYQRMMNDARNHRFDLLVIWKLTRLGRNMLDVLQTVEEL
                     LRYDVGLHSISEQFDVTTSTGKLMLQLLGSFGEFERNQIAENVQMSMQSLVKDKKRYA
                     GGRRLGYVSGIDTNGQKQLVIEPEEAKIVRLIYAKYLGGAGYRAIANFLNRQGYQTVK
                     KNAFSTTAVKDILRNKIYAGYLEYARYINWETKRRKGKNPRPILVKGTHEPIISEEDY
                     QKVQERLALEGKQPKWNHTGENVLTGLLRCPECGAPMAASNVTNTLKDGTKKRIRYYS
                     CSVFRNKGASVCHANSIRAEQAETFVADRLKEIVQLPEVLPRLVAALNEEIVRQSQPL
                     EQELVVLLERKEELKTKIEKWEAALEDSPELFPMLKDRLDELTEKRRQLHIRENEILG
                     IFQQGEPIQVKDVQRILTSLDRFLAQSEKKQIKALYRTFIEKITFDPNHKEMLEITMK
                     FTHAVVQQLNEQYQIAVSKTKDTAIFVLKTPFTLTI"
BASE COUNT         2218 a         1048 c         1282 g         1838 t
ORIGIN      
        1 gttgtgattt ccaaaatcac aacttttttc ttttgtcaag attttgagtg ggatatattt
       61 ttgtagaact gaagatcatg aaattatgag aggttggagg taatgtagtc atacaaataa
      121 aaatttttta gaaagtgagt ggtaattaat gaaaatgaat cacacttcaa ttcagaagca
      181 tttacaactg ttaaatacac tggggatttc ttatgttaat ttgagaactt tttctacatt
      241 aaacaagaaa accgtttctc atattttttc tactgcagat tctcaagcaa ttattaacta
      301 tttagaaaaa cagaaattgt ctgaaggtac agtaaacata acttataatc ctattaaaca
      361 agaggtgatt acacaaaaaa agcattctgt gagagacaat gatattgaaa aaattcggtt
      421 tgtatttatt gatattgacc ctaaaaggaa agagggaagt gcaacagata gcgaaaagaa
      481 aaaggcagaa aatgttatgg aacaagtaga acgttatttg aaagaaaaga gaatagaatc
      541 atttgtaaaa gttgatagtg ggaatggcta ccatattttt cttcccataa atgaacaacc
      601 aaataatcac gagacgattc ttactattca gaattttctt caattacttc accgaagatt
      661 tggagtagaa gatgaagttg atattgatac ttctgtttat aatccttcaa gattatgcaa
      721 attagttggt acaccagcgg tcaaaggaaa gtccacaaaa gagcgtccgc atcgtttatc
      781 tagtttcctt tctattcccg ataacataga tagaagtaat tgttttaaaa ttcttgaaca
      841 gatagttgaa gaaaattcca tgatgtcaac ctctagagta aaaagccatc catcttttag
      901 agaagtgtta ccttttattc aagcagacta taaacaatgg ttagctcact atcctgaatt
      961 aacttatacc ataaaagagg gcgatcataa aggagttacc ttacttatat ttgacgaatg
     1021 tccattaaga aaacacacga ataattctaa tggttcttgt ttgtctgtta cggctgataa
     1081 taagattcga tttcattgtc tgcatgcttc ggataaaaat aaagatattc aagactttgt
     1141 aaaaaaatat ccgttgccaa aacctgtaaa tccttctgta gaatcattag aatccaatca
     1201 agaatattat tttgaccagt ttaaactgaa tcaagaagga gtatttttgg tgaaagaagg
     1261 tcaagaagaa aaagttgctt gtccgatgtt tattaagcaa atacgcaaaa atatcgaaca
     1321 aaaagtcatt acacttgttc ttgcctatta tgtcgatcat tgttgggatg aaatagaaat
     1381 agaaggtgga tgtttacaaa ctaataattt taaacaatta gcaaaacatg gcttaatcat
     1441 taaagctaga agagaagctg aagtcgttga ctttttacag attcaacggg agaatgctcc
     1501 aatctttttt gaacatgaat ctcttggctg gaaaaaagaa aatgaagcaa tgatttttca
     1561 gttacaagat tcttttggac gtgaaattcc ttctgtatta actaaaaact catgctacca
     1621 attaacaact aatggtacag aagcggaatt tgacgcattg gttcataagt atgttttagg
     1681 aacgaactta gaacttgctt ggtcgattgg tattgcttct attgtgttag gctatttaaa
     1741 cgttacgaag gctaatactt ttttgagttt actaattaac ctacagggca agagcacaac
     1801 gggtaaaacc actacgttac actttattgc ctctttattt ggaaatccaa ctacggttct
     1861 tagaaatatg aatgctacca ataaagccat tataaagcta gcatcaaaca atcagggagg
     1921 tgttccacta atactagatg agttaggagc agcaactcaa aataacttta gctcgttgat
     1981 ctatcaatta gcttcaggag aagaaaggct aagattagat tctaattgtc agcttatgcc
     2041 acaagagact ttttctgttt tgacccttac ctcttctgaa gagcgtttgg aaaattattt
     2101 caatgagaaa ttaactggtt tgaaagttcg atatatcgaa ttttctgata tcaagtttac
     2161 aaaggatgcg aatcacgcag agaaagttaa aagtctttct tctaatactt ttggttgggg
     2221 tataaagtta tttcttttga aactctttga acaagggatt acaaaaattg aagcaacttt
     2281 tgtcgaagct caaaaaaaac tatgggctgt tttacctgaa cattctttga agcaacgcat
     2341 tggtaataat ctagctttga tttatagtgg agccctccta attcaagata tactgggcta
     2401 tcctttacga ttagagcata tacagacggt gcttattaat tcgtacgaac agttaattgt
     2461 agatactagt ttaggaagtg agcaaacggt atttgaccga gtgaaaacat tgttggtgat
     2521 gaatagccat aaatttttaa caaacagtga caacaaacat acgcccagta cgatttgggg
     2581 aaaagtatca attcaaaaaa atggggatat taaggtaaat atttttccta tcccgtttga
     2641 ggacttgtta aggcgagaat ttcaagtaaa ggatctaacc tatcttttta ggcaattgct
     2701 tgattcgggt aatatgtgtg cagaaaaagg gagaagaacg aaacgtattc acatgaatcg
     2761 tcaagttctt ccaacatacg agattctatt accttcttca ctcaaatcgt attttcgatt
     2821 gtaatttaga ataaaccaaa gtatgaaagt gaggcaccat gttatggaaa tatttcttat
     2881 tgatttagca aaacaactga ttacttatct gtatgatagc gagcaaaccc aaacggaagt
     2941 aaatgaagct tgatctatgc ttcatttact tcattcaatc aaacatgaaa gaaaggaaaa
     3001 ttctaatgac taaaaataaa atgacactaa aagcagaggt tcttctgtat attcaagaac
     3061 atttttcaaa tcaagccttt tttacaaaac caatttactt ggatttcgaa ataagagggg
     3121 tatctgctgg atctatcggg ggtactcttc aagcgttgaa gaacgaagga tatttagaaa
     3181 atcactttgt gcaacgttcg ttcaatagaa gagtcgtgaa aaaatggtat ttagttcatt
     3241 catagagagg ggaatcaagc atgcgtgtgg cttgttatga tcgaacgtct gtttatgaag
     3301 aagcgaaaat tagtaatgac gctcaatatg atgcagtgaa agagtatgtt ttagagaaat
     3361 atgaacaaat tccccaacga ttttcagaca aaggagaatc aggggaaaaa gcggagcgtc
     3421 cagcttttcg acagcttaga gcttgggttt gtgcgtcaga agaagagggg gtgatctatg
     3481 tttggcgtta tgatcgattg gcacgtcaag ccaaaatagc cttagaattt attgagttgt
     3541 gccaacaaca tcaggttcaa gtgtattcga tttctgaacc tcttcctgct tctttctcac
     3601 acgcgtcaac gaataaacta tttgtgcaat tattgttcac ttttgcagaa caacaacgaa
     3661 acgtcattat tgagaatgtt cgtgccggac tttcctataa aaagcaacaa cggcaatatt
     3721 tgtcttctgc ggtaccatat gggtatcgtt tagtgaatgg gaaaatccaa caagaagtac
     3781 acgaagcaag gattgttcgc tatatttttc aactttattt gaccaaaaaa tatggctaca
     3841 aaaagctttg tcaacgattg acgcaacaaa agcttttctt ccgcgaacgt ccttttcagc
     3901 cctatcatat ttatagtatc ctaaaaaatc cattgtacta tggcgaaatc aaaggaggat
     3961 ctttggggaa gtatctagga acctttgaac cgattctctc gaaagcaacc tttttacaag
     4021 cccaagagat tcgccaatca aggtgtacag ctaaaaaaga tacgtatctg tacttattac
     4081 gtcaaaaaat tcggtgtccc ttctgtggtc gtcatctgag ttcgaagtat caatggaata
     4141 caaagaagac gaagacgctt cattattatc attgtaccga ccgaagctgc caaggaattt
     4201 ttcttcgggc agaaagggtt gagtcagaag tattggaaaa gatacaggct ttttttcaat
     4261 acgaaggcgt ctatcaagcc ttgattgccc aattaacgca acaattagcc gaaaagcgaa
     4321 aagatgaaaa acgcaaagta atccaacaaa agaaacaaga agcacaatgg attaaggcat
     4381 ttgaagaggg caattgttca ctggaagaac tcaagcaaca actgcaagct cttcaaaagc
     4441 cgatgtctac ttcttcgttt ctttcactcc aacagtgtga agcaaaagtg aaagaatggc
     4501 ttcaactaaa agacctctct agtcgacaat tattatgtga ctatgtttgt cggattgagg
     4561 taacaaagga taaaacagtt tgtgggattt acttccgaca actgacacaa aatattttag
     4621 aaaggaacga atgatctatg gaaatgatac aaaccaaacg agtcgcattg tatattcgcg
     4681 tgagtacaca agaacaagct ttagaaggac atagtattca agcacaagaa caaaatggac
     4741 gacagtttgc cgaacggatg ggctatgaag tggtagaagt ttatgccgat gaaggaatca
     4801 gtgggaaatc cacaaaaaat cggttcgcct atcaacgaat gatgaacgat gctcggaacc
     4861 atcgttttga tctactagtg atttggaaat tgacccgtct cggacgcaat atgttagatg
     4921 tcttacaaac agtcgaggaa ttattgcgat atgacgtagg attacattcg atctcggaac
     4981 aatttgacgt gaccacttcg acaggtaaat tgatgttaca actccttggg agtttcggtg
     5041 aatttgaacg caaccaaatt gcggagaatg ttcaaatgtc aatgcaaagc ttagtgaaag
     5101 ataaaaagcg ttatgcagga ggccgacgtt taggctatgt cagtggtatt gacacgaatg
     5161 gtcaaaaaca attggtcatt gaaccagaag aggcgaaaat tgttcgtctg atctatgcaa
     5221 aatatttagg cggagcaggg tatcgagcga ttgccaattt tttgaatcgt caaggatatc
     5281 aaacagtcaa aaagaatgct ttttctacca cggcagtcaa agatatctta cgcaataaaa
     5341 tttatgcagg gtatttagaa tatgcgcgct acatcaattg ggaaaccaaa cgacggaagg
     5401 ggaagaatcc gagaccgatt ttagtgaaag gaacccatga accgattatt tcggaagaag
     5461 attatcaaaa agtacaagaa cgccttgctt tagaaggaaa acaacccaaa tggaaccata
     5521 caggtgaaaa tgtattgaca ggcttgttac gttgtccaga atgtggcgca ccgatggcag
     5581 cgagtaatgt cacaaatacc ctaaaggatg gcacaaagaa acggattcga tattattctt
     5641 gttcagtctt tcggaataaa ggagcaagtg tgtgtcatgc caattcgatt cgtgccgaac
     5701 aagcggaaac atttgtcgcc gaccgattaa aggagatcgt tcagctacca gaagttcttc
     5761 cccgattggt ggcagcgtta aatgaagaaa tagtgcgaca aagccaacca ttggaacaag
     5821 aactcgtggt gttactggaa cgaaaggaag aattgaagac taaaattgaa aaatgggaag
     5881 ctgctttgga agatagtcct gaattgtttc cgatgttaaa agaccgctta gacgaattga
     5941 ctgaaaaacg acgacagttg cacattcgag aaaatgaaat cttagggatc tttcaacaag
     6001 gagagccgat tcaagtcaaa gatgtccaac ggatattgac tagtttggat cgttttcttg
     6061 cccagagtga gaagaagcaa atcaaagcct tgtatcgtac ctttatagag aaaataacct
     6121 ttgatcctaa ccataaggag atgttggaaa ttacgatgaa attcacgcat gccgtggtac
     6181 aacaattgaa tgagcaatat caaatagccg tatccaaaac aaaggatacg gctatttttg
     6241 tgttaaaaac accctttacg ttaacgatct agtctaggtt atatcaaaca acgttagagg
     6301 ggcgctaacg cttctctagc gatttgaaat cgttagagaa gtagttaggg aggtccttat
     6361 cataaatagg gatatttatt aatgaa
//
LOCUS       DAZKLD010000142         6354 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.143, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000142 DAZKLD010000000
VERSION     DAZKLD010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6354)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6354)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6354
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.143"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            214..1089
                     /locus_tag="ACG8KR_002490"
     CDS             214..1089
                     /locus_tag="ACG8KR_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFQ0201807.1"
                     /translation="MKESASCFVLSYRGGELTVYVMKKLNHNAVLIDDDGIEKIAMGK
                     GIGFSAKVSKPFDVEDADKLFVLDSKEKIKRFSEIVSQIPIEFMDFAEEVIELITARI
                     SSPLDSNIYITLTDHIYFAIQRKREDRDVTAIMLPEMRFLYPNEYDIAVEVVERINHR
                     YQTELGSNEVGFITMHIINAELGEPNSLKSLKIVEMTTTILSHLEKSYSNVYDKESLT
                     YHRLMVHIKFLVKRLIYQEETPVENIGFFTETFKHSKAYCTALKIKDIIEQQFQLAIQ
                     ENEIIYLAIHLARIY"
     gene            1193..1957
                     /locus_tag="ACG8KR_002491"
     CDS             1193..1957
                     /locus_tag="ACG8KR_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate deacetylase"
                     /protein_id="HFQ0201808.1"
                     /translation="MGKVIFNSDDFGYSHGVNYGIMDAYQRGILTSTTLMANMPGFEH
                     AVKLRKEMPRLGVGVHLTLTCGKPLLKTVDTLIESGEFRSLSYYQHPFNIDKDQLYQE
                     WNAQIQKIYHAGIIPTHLDSHHHTHTLDENQEVVVTLAKKYDLPVRSNFERKDEVRHV
                     DYFEPCFDVVGETDEKKRQINTSVEEYLASLLMILREDKTIEVMCHAAYLDQELLNGS
                     SFVYPRTHQVEFLIHSAFAQKVKNDPLIELVTYEDI"
     gene            1944..3386
                     /locus_tag="ACG8KR_002492"
     CDS             1944..3386
                     /locus_tag="ACG8KR_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFQ0201809.1"
                     /translation="MRIFNRGGIKMNVKSNVQKLGRAMLLPVAAMPLAGLIMRLSADD
                     MLNIPVIGAAGNAVFGNLDILFAIGVAIGFVKNKDKGIPALTGFLAIATLKKGLEIMD
                     PEVNMGIFGGIISGLVAAWTYNRFKEQKLPVVFSYFAGEKFPLTMVMILQTIAAVFLG
                     FIWPLIQIGIDNFAKLLVDMGALGVGIFMFLNRLLIPFGLHHVLNTYIYYDLGSYTSP
                     SKEVFRGEMTRFINGDPQAGLFLSGFFVVMMFGIPAICLAIYKAAYKENKEMVKGIMG
                     SGAGTSFIANITEPVEFSFMFLSPMLYVIHALFAGLAGIVCYLFNIRIGFTFGACIVD
                     YLINFRIATNAILILPIGIFFFALYYVTFYYLINKRNIQTLGREAKAEFGNEVTLEET
                     ELGLASKNYEYMATKMLQAFGGKANILDVYSCNTRLRVEVVDPTMVEEQRIKQLGISG
                     IIKPTEKNYQIIIGLEVTYVMAEFNKLLEE"
     gene            3397..4032
                     /locus_tag="ACG8KR_002493"
                     /pseudo
     CDS             3397..4032
                     /locus_tag="ACG8KR_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361883.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
     gene            4123..5082
                     /locus_tag="ACG8KR_002494"
     CDS             4123..5082
                     /locus_tag="ACG8KR_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFQ0201810.1"
                     /translation="MTYTHLTTDELVIIESYFKMNQSVAKTAHCLNRSRQTIHKVYLF
                     FKQGKSALEYYQQYKKNKSNCGRRPLVLPEEQSEYIQRKVVQGWTPDVIVGRAAFPIS
                     CSARTIYRMFKKGLFDSSDLPMKGKRKPNGHQERRGKQTFRRSIHEREKDYSQFSNEF
                     GHLEGDTIVGLKHKSAVITLVERLSKVIITLKPCGRQAIDIEKKLNQWFESVPKNLFK
                     SITFDCGKEFSNWKQISNVNDIAIYFADPGTPSQRGLNENSNGLLRRDGLLKSMDFNS
                     VDEFFIQSVASKRNNIPRKSLDYRTPLEVFLSYVSIDDLSNLI"
     gene            5215..5421
                     /locus_tag="ACG8KR_002495"
     CDS             5215..5421
                     /locus_tag="ACG8KR_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HFQ0201811.1"
                     /translation="MNNRTFDILKWIAIVVIPALATFVGLVGKGVNWRYTDITVMIIT
                     GFGAFLGSILGVSNRTYKKISSDK"
     gene            5432..>6354
                     /locus_tag="ACG8KR_002496"
     CDS             5432..>6354
                     /locus_tag="ACG8KR_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFQ0201812.1"
                     /translation="MKKKITITAMSLLTALFLLPINGFAYTINNEFNLGANEGSSQVA
                     NNQYILLHETANETATGRNEAQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGS
                     YANANSPVQIELQHTHDKATFEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHL
                     WVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAAN
                     PTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIY
                     RSDVNQAYNTSGNVGYHVSFNMRNFPNKKVYV"
BASE COUNT         2130 a          978 c         1315 g         1931 t
ORIGIN      
        1 ttttttttgt ttttatgtaa ttacctccaa atcaattcga tacaagtatt atcataaaac
       61 gagtaaaaaa gatattgata acgcttttat tatgtgttaa actatttttg taaattatgt
      121 agggtgctac tattcaatta ggcacgacct aagaaagggt tctttcttag gtcgtttttt
      181 gtctttaaaa attgtttgga gacaaaaata tgtatgaagg agtctgcaag ttgttttgtt
      241 ttatcatata gaggaggtga attaacggtg tatgtgatga aaaagctgaa tcataatgct
      301 gtattgatcg atgacgatgg aatagaaaaa attgcgatgg ggaaagggat tggcttttct
      361 gctaaagtga gtaagccatt tgatgttgag gatgctgaca aattatttgt attagattct
      421 aaagaaaaga tcaaacgatt cagtgagata gtctcgcaga ttccaatcga atttatggat
      481 tttgcggaag aagtgattga actgataaca gctaggatct cctctccatt agattcaaat
      541 atctatatta cattgacaga tcatatttat tttgctattc aacgaaaaag agaagataga
      601 gatgtcacag caattatgtt gccagaaatg agattccttt atccaaatga atatgatata
      661 gcagtagaag tggtcgagcg aatcaatcat cgatatcaaa ccgagttagg gtcaaatgaa
      721 gtaggattca tcactatgca tatcattaat gctgagctag gagagcctaa tagtttaaag
      781 agcttaaaaa tagtagaaat gacgaccacg attttgtccc atctagaaaa atcctattca
      841 aacgtttatg ataaagaatc actgacttat catcgcttaa tggttcatat caaattcctg
      901 gttaaacgct tgatttatca ggaagaaacg ccagtagaaa atattggttt ttttactgaa
      961 acatttaaac atagcaaagc gtattgtaca gcactaaaga ttaaagatat catcgaacag
     1021 caatttcaac ttgccattca agaaaatgaa atcatctatt tggcaattca tctagcaaga
     1081 atctattaaa ataagggcgt aactatctat aggcgcaacc aaatgacaca tagtaaatgg
     1141 actatgatta tttggttgcg cctattttag tagaaaaaag aaggagaatg aaatgggaaa
     1201 agtgattttt aattcggatg attttggcta tagccatggt gtgaattatg ggattatgga
     1261 tgcttatcag cgaggaattt tgacatcaac tacattgatg gcaaatatgc cgggtttcga
     1321 gcatgctgtg aaactaagaa aggaaatgcc taggctaggt gtaggagtcc atttaacatt
     1381 gacgtgtggc aaaccactcc tcaaaactgt cgatactctt atcgaaagcg gagagttcag
     1441 atccttatct tattatcagc atccatttaa cattgacaaa gatcaactgt atcaggaatg
     1501 gaatgctcag attcaaaaaa tttatcatgc ggggatcata ccaactcatt tagacagtca
     1561 tcatcatact catactttgg atgaaaatca ggaggtagtt gttacattag caaaaaaata
     1621 tgatttgccg gttcggagca actttgaacg aaaagatgaa gttcgtcatg tcgattattt
     1681 tgagccttgt ttcgatgtcg taggagaaac tgatgaaaaa aaacgacaaa tcaatacgag
     1741 tgtggaagaa tatttggcaa gtttactcat gatattgaga gaagataaaa cgatcgaagt
     1801 catgtgtcac gcagcgtatt tggatcagga attgttaaat ggttcaagtt ttgtgtatcc
     1861 tcgtacccac caagtcgaat ttttaattca ttcagcattt gctcaaaaag tgaaaaatga
     1921 tcctttgatt gagttagtaa catatgagga tatttaatag aggaggaata aaaatgaatg
     1981 ttaaatcaaa tgtgcagaag cttggaagag cgatgctact tccagttgca gcgatgccat
     2041 tagcgggatt gatcatgcgt ttatcagctg acgatatgct gaacatccca gtaattggtg
     2101 cagctggaaa tgccgttttt ggaaacttgg atatcttatt tgctatagga gttgcaatcg
     2161 gttttgtcaa aaataaggac aaaggaatac ctgcacttac tggtttttta gcgattgcta
     2221 ccttgaaaaa aggcttagaa attatggacc cagaagtgaa tatggggatt tttggtggaa
     2281 ttatttccgg tttagtagcg gcatggacgt ataatcgctt taaagaacaa aaattaccag
     2341 tagttttttc ttattttgcg ggcgaaaaat tcccattgac tatggtgatg attctacaaa
     2401 caatcgcagc tgtttttttg ggctttatct ggccgctcat ccaaattggg attgataatt
     2461 ttgcaaaact gctcgttgat atgggagcat taggtgtggg gatatttatg tttttgaatc
     2521 gtttgctgat cccctttgga ctgcaccatg tgttgaatac gtatatttat tacgatttag
     2581 gaagctatac atctcctagc aaggaagttt tcagaggaga gatgacacgc tttatcaacg
     2641 gagatcctca ggctggactg tttttatcag gtttttttgt agtaatgatg tttggtatac
     2701 cagctatatg tctagctatc tataaagcag cttataaaga aaataaagag atggtcaaag
     2761 gaatcatggg aagcggtgca ggaacttcat ttattgcaaa tatcactgag cctgttgaat
     2821 ttagtttcat gtttctttct cctatgttgt acgtgatcca tgcacttttt gccggtttag
     2881 cagggattgt ctgttactta ttcaatatac gaattggttt tacattcgga gcatgtattg
     2941 ttgattatct catcaatttt cgaatcgcta ccaatgctat tttgattctt ccaatcggaa
     3001 ttttcttttt tgccttgtat tacgttactt tttattattt gatcaataag agaaacattc
     3061 agacattagg acgtgaagct aaggcagagt ttggcaatga agtaacctta gaagaaacag
     3121 agttagggtt agcaagtaaa aattatgaat atatggcaac aaaaatgcta caagcgtttg
     3181 gaggaaaagc caatatcctg gatgtctata gttgtaatac acgcctacgg gtagaggtag
     3241 tggatcccac catggtcgag gaacagcgaa tcaagcaatt agggataagc gggatcatta
     3301 aaccaactga aaaaaattat caaattatca tcggcttaga agtgacttat gttatggcag
     3361 aattcaataa attattagaa gaatagaaag gaaaagatga tgatgaaaaa acagattatc
     3421 actatagctg gaggcgggag tacgtataca ccaggaatcg ttcaagccat tttgaacaat
     3481 gaaaaacggt tgccattatc tgaaatacgt ttatatgata ttgatgaaga aagaaatatg
     3541 gatatgtact tgattgttaa atttatgctg aaaagaaaag gtttttcaaa tattcgcatt
     3601 cgtgcaacgg atgatccaaa gcttgctttt acaggatgcg attttgtttt ctcacaaatt
     3661 cgtgttggag gattggagat gcgagaaaag gacgaaaaga ttccacttaa acatggatta
     3721 gtaggacaag aaacgtgtgg actcggagga tttgcttatg ggatgcgctc aatcaaaggt
     3781 ttattggaaa ttgtcgatca tatacaagac tatgccccca aagcatggat tttgaattat
     3841 acaaatccag agtcgatcgt ttctgaagct gttcgtcgta aatatccgaa tgtaaaaatg
     3901 attaatgcct gtgatatgac tatttcaatt gaagaaacga ttgcagtgaa ttacggttat
     3961 gatcgtaaga attggatcgt tacgtattat ggtttaaacc actttggctg gtatacatcg
     4021 atttatgatc gccgattgta aaattaagct agacaactaa aaaagtcatt tgtgctacac
     4081 tcaaaatagt tccgaccaaa gaattataag gagtgagtac aaatgaccta tacccatctt
     4141 acaacggatg aacttgtaat aatagagtct tatttcaaaa tgaatcaatc tgttgctaaa
     4201 actgcccatt gcttgaatcg ttcaagacaa acgatccata aagtatatct gttcttcaag
     4261 caaggaaaat cagccttaga atattatcaa cagtataaga aaaacaaatc aaactgtggt
     4321 agacgtccgc ttgttttacc cgaggaacaa tcagaatata ttcaaagaaa ggttgttcaa
     4381 ggatggacac ccgatgtgat tgttggtcgt gcagcgtttc ctattagttg ttctgctcgt
     4441 accatttatc gtatgttcaa aaaggggcta tttgattctt ctgacttacc gatgaaaggc
     4501 aagcgtaaac cgaatggaca tcaagaaaga cgtggaaaac aaactttccg ccgctctatt
     4561 catgaacgtg aaaaggatta tagccaattc tcaaatgagt ttggtcacct tgaaggtgac
     4621 actatcgtag gtctgaaaca taaaagtgct gtaattacct tagttgaacg attatcaaaa
     4681 gttatcatca cattgaaacc gtgtggtaga caagcgattg atattgaaaa aaaattaaat
     4741 caatggtttg aatctgtacc gaaaaaccta ttcaaatcca tcacttttga ttgtggaaag
     4801 gaattttcaa attggaaaca gatcagtaat gtcaatgata ttgccattta tttcgctgat
     4861 ccaggaacgc cgtctcaaag aggcctaaac gagaattcta acggattgtt acgtagagat
     4921 ggtttattga aatctatgga tttcaattca gtagatgaat tttttattca atctgtcgca
     4981 tctaaacgaa ataatattcc tagaaaatca ctggattatc gaacaccttt ggaagtattt
     5041 ttgagttacg taagtattga tgatctgtct aacttaattt gacaattaag aaagataaaa
     5101 ttgaacaact ggacgggcaa attgatgcct tgctttcaga aagagaaata ttgatagaag
     5161 aaaacaagcg attgaaaaag aaagtagccc aaccaatgaa gggaggtgga tagtatgaac
     5221 aatagaacct ttgatatttt gaaatggatc gctatagtag ttatccccgc tttagcgact
     5281 tttgtcggtc ttgtcggaaa aggtgttaac tggcggtaca cagatataac ggtgatgatt
     5341 attacaggtt ttggagcatt tctagggagt attttaggtg tatcaaatcg aacgtataag
     5401 aaaatttcat cagataaata ggaggataga tatgaaaaag aaaattacta ttactgcgat
     5461 gagcctgtta acggctcttt ttttattgcc aattaacgga tttgcctata ctatcaacaa
     5521 tgaatttaat ttgggcgcaa atgaaggtag ctcacaagta gcaaataatc agtatatttt
     5581 actgcatgaa acggctaatg aaacagcaac aggacgcaat gaagcgcagt atatgcaacg
     5641 ttcatggact agcgcttata ctgcttatat tgtgggagac ggcggaattg tttatcaagt
     5701 cggtcaacct ggttatgtac agtacggtgc tggttcgtat gctaatgcca acagtcctgt
     5761 gcagattgag ttacaacaca cacatgataa agcaacgttt gagaaaaact acaaggcata
     5821 cgttgaattg gctagagatt cagcaatgaa atatggtatt ccattaacgt tggacactcc
     5881 ttataaccaa ccgggaatca aatcgcattt atgggtaaca caaaacatct ggggcgatca
     5941 tacagatcct tacggttatc tttctgaaat gggcgtaagt aaagaaaaat tagcatatga
     6001 tttagctcat ggatttaccg atgaaaatcc aacaacttct gaaaacaagc ctgtcattga
     6061 tccaacacga gctggtgcag ctaatcctac actgacagat ggaacgaatt acgcccacat
     6121 tgatcagttt ggagaaatcg aaaatgcaaa tttgcatgta gctggatggc acattgctaa
     6181 ctataaatac gagtatatct tcattatgga ttacaatact gggaaagaat tagctcgagt
     6241 aagagctgat ggaatttata gatcagatgt aaatcaagct tataatactt ctggaaatgt
     6301 tggctatcat gtatctttta acatgcgtaa cttccctaat aagaaagtct atgt
//
LOCUS       DAZKLD010000143         6326 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.144, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000143 DAZKLD010000000
VERSION     DAZKLD010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6326
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.144"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..208
                     /locus_tag="ACG8KR_002497"
     CDS             <1..208
                     /locus_tag="ACG8KR_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0201813.1"
                     /translation="AESFCERYPEYKLSFLSENKMIKVEFSSQFLLSKVYSILLEGTI
                     GYILLDSLYKGTYQSLENLSQKII"
     gene            340..1368
                     /locus_tag="ACG8KR_002498"
     CDS             340..1368
                     /locus_tag="ACG8KR_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0201814.1"
                     /translation="MYWQSFDENHSDILSSIKNKRQLLEKFAKHAPYTRSIDRKKWIS
                     LLRISLQRMKQGHSITYVPEEMNKFVHPLITKEEFTHYILEPFFEANFWSMNHITPTE
                     QAYLYFMFGVMNTYLEEDLLDTPDKVQESWGWIESSIVENFEKNFKICFTTSEKNYLY
                     VNLAMIHLYSRVFGTKKKVDAFGKSAEDRDFQHIFPVMYPIMQQFFSGVARRIPELNT
                     YYEKNRRLIFQYCMLVRDIFIKHEQPVIFYIQSKYGKTQELWAKKRILSMTERPIKYS
                     ASADQLPDIVISDYPIDKKAYDQTNTQIFYWNGQPTKNDWARLKAEIIKIRNEKNRSI
                     LEKLDFVN"
     gene            complement(1394..1834)
                     /locus_tag="ACG8KR_002499"
     CDS             complement(1394..1834)
                     /locus_tag="ACG8KR_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFQ0201815.1"
                     /translation="MMIKLYTGSGCSSSRRAKRWLIVHDIPFIEQNLIRQPLTKNEFI
                     HLLKLTNNGVTEMLSTHSISYKNLVHRIDELSLSELYIEIRTSPSLLKKPIIYDQSAK
                     LLTGFSEDKIRMFLSKTKRKQELNELLLSGHLVDFKENNLFAMS"
     gene            1994..2500
                     /locus_tag="ACG8KR_002500"
     CDS             1994..2500
                     /locus_tag="ACG8KR_002500"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HFQ0201816.1"
                     /translation="MSNTADAIVVIDLQKGVCFDSSPIHELETVTALVNKRIKTYETI
                     GKPVIFVQHEDAQLVPQTKPWELLSELSIPEHSYFVRKTHANSFYKTNLSVLLTELDC
                     HSIELCGAQAEYCIDTTVKFAHGLGYQLMMCRGATTTYDNPFMKAKETIAFYEKIWDK
                     RFLTFLEQ"
     gene            2668..4581
                     /locus_tag="ACG8KR_002501"
     CDS             2668..4581
                     /locus_tag="ACG8KR_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFQ0201817.1"
                     /translation="MNSLRTKQVLKGALVILFAVSTSVPQWTVYGESISSESESREVE
                     TTETTDAGNETKSSTSQTETNTETTVETSVTSTDQETTATTDNQQTEEATATKMSNDE
                     SSSAKVSDKENGTENDDIGKGENFNGKEYLNYFGKQMTEILNGPTEDIKSKLIELLVT
                     ELAVNDYGVDKEIIKHLIATYLLYDIAFAKNSVLMSMLGGTDDGTTPTENTGFYPNTK
                     QLHQTVLNKQDGKMMDVYAYYVDQGSDKTVMIHGGFRGNWNNGIVTEEYNDFYKAGYN
                     LLFVDSRATGNSGGDYVTYGQYESDDVLYWINQEVRERPSQKILLYGGSMGAATMMSV
                     LAKDIPVNVKGIIENCGFASIDEQLRFTYSQTVAPALPDAIKNQLDIIGDQEHEDLFM
                     GLLKQYYFDQEMHLDTKAALPTIGMSDSLPKLIIHGTADDVVPVSNAQKLYELSGGYK
                     DLLLVEGAGHGKAQEVDHAAYTKHVTDFLKVVFHDQINVKYVDENNKSLLKDQDEIQL
                     YGAYGENYMTEQKTFEGYELANVEGPTEGIFNETIPTIIFYYKKIPVAPPKKQDPTEN
                     ADNKGKNEPREDQSSDDTQPIQMTVKVQKKTDAKKRMLPKTGEKKHSLLIYSGGFLSV
                     STIITWIWRRKRG"
     gene            4586..5587
                     /locus_tag="ACG8KR_002502"
     CDS             4586..5587
                     /locus_tag="ACG8KR_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFQ0201818.1"
                     /translation="MKWFKRIVIAVPVIIVVILVTVFLINQITPKPISLLVRKQFDTS
                     EKEQVYARPDDFQQKIEQIDIKKEISYPSKYKNNNMDIYTPKVEKKKLPILFWMHGGA
                     YVGGDKNDCRDYLEYLCSDTQQIIVNIDYERSPEAKHPTPVIQLNEAIQAAKKEIKDQ
                     ADWSNISIGGNSAGAQISGEYLLALQNEEIKKADHLDPTLENKQITKFISLSGLLDPS
                     ALTQVSDKISSFLYEKCGWAYFDNKDFEQSEKVKSLALVRHADRWTQNTFLTDGNTNT
                     FTKQMEEVTSVFEKAGVKVTKVDYAEKNAVLNHEYQFDFSNKQAQETYQKLVMFFKE"
     gene            complement(5789..5995)
                     /locus_tag="ACG8KR_002503"
     CDS             complement(5789..5995)
                     /locus_tag="ACG8KR_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HFQ0201819.1"
                     /translation="MKAIHISLLFFASALILFAIHLCSQPYINNNGILVEPAFFCLPL
                     GFLSLLASAGFGFVAFFKRSKQQI"
BASE COUNT         2338 a          961 c         1243 g         1784 t
ORIGIN      
        1 agcagaatcg ttttgcgaaa ggtatccaga atataaatta tcttttcttt cagaaaacaa
       61 gatgataaaa gtcgagtttt cttctcagtt tttactgagt aaagtttatt ctatactttt
      121 agagggaacg attggttata ttttactgga ttcactttac aaaggtactt atcaatcctt
      181 agaaaatctt agtcaaaaaa ttatataagt ttgcgtacga ttcaaagaaa attaagagaa
      241 cttaatacta ttttgaaaaa ctatcattta tcttgcagct tgaaaaaagg taatccgata
      301 gaaggagaag aataccgtat ccgatacttt tttcacttga tgtattggca gtcattcgac
      361 gaaaatcatt ctgatatatt atcaagtata aaaaataaac gtcaactatt agaaaaattt
      421 gcgaaacatg cgccatatac gcgtagtata gatagaaaga aatggatcag cctactaaga
      481 atttcgttgc aacgaatgaa acaaggccac tcgattacgt atgtcccaga agagatgaac
      541 aaatttgtgc atccattgat aacaaaagaa gagtttacgc attacatact agagcctttt
      601 tttgaagcaa acttctggtc gatgaatcac atcactccaa cagagcaagc ttacctttat
      661 tttatgtttg gtgtaatgaa cacctatttg gaagaagatt tgttggatac tccagataaa
      721 gtacaagaaa gttggggctg gattgaatcg tctattgtag aaaattttga aaaaaacttc
      781 aagatttgtt ttacaacttc agaaaaaaat tatctatatg tcaatttggc aatgatccat
      841 ttgtattcaa gggtttttgg gacaaaaaag aaggtggatg cttttggaaa atcagcagaa
      901 gacagagatt ttcagcatat atttccagtg atgtatccaa tcatgcaaca gttttttagt
      961 ggggtagctc gcagaattcc agagcttaat acttattatg aaaagaacag acggttgatt
     1021 tttcaatact gtatgttagt cagggatatt tttataaaac acgaacaacc tgtcatattc
     1081 tatattcaat caaagtatgg taaaacacag gaattatggg caaagaaaag gatattatct
     1141 atgactgaac gcccaataaa atatagtgca tccgctgatc agttacctga tattgtcatt
     1201 agcgattatc ctatcgacaa aaaggcatat gatcaaacga atacacagat attttactgg
     1261 aatggacaac ctacaaaaaa tgattgggcg cgtctgaaag ctgaaatcat aaaaatcagg
     1321 aatgagaaaa atagatccat attggaaaaa ttggatttcg ttaattaaga aaagcagatt
     1381 gtttatgcca aaattaagac attgcaaaaa gattattttc tttaaaatcg acaagatgac
     1441 cagaaagcaa gagttcgttg agctcttgct ttcttttagt tttagaaagg aacatcctga
     1501 ttttatcttc gctaaatcca gtcaaaagtt ttgcagattg gtcgtagata atcggttttt
     1561 tcaataaaga aggggatgta cgaatctcaa tatatagttc actcaaactg agttcatcta
     1621 ttctgtgaac aagattttta taactaatag aatgagtaga gagcatttcg gtcacgccgt
     1681 tgtttgtcaa ttttagtaag tggataaatt catttttggt tagtggttga cgaattaagt
     1741 tctgttcgat aaacggtata tcatggacga taagccaacg ttttgcgcgt cttgaagacg
     1801 agcagccaga acctgtatac aatttaatca tcatacattc ctccaatagc taagtattta
     1861 tgaaataata acatgaagaa atagtcgaaa agtgccattt agaggtgtaa aataagaaaa
     1921 tgttgtcacg tataatggaa ctagctagat aagaaatact attaaatcaa gaaaaaagaa
     1981 agaaggcaca agtatgtcaa acacagccga cgcaatagta gtgatcgatt tacaaaaagg
     2041 ggtatgtttt gattcttcgc cgatacacga attagaaaca gtgacagcat tagtgaataa
     2101 aagaatcaaa acatacgaaa cgattgggaa accagttatt ttcgttcaac acgaagatgc
     2161 tcaattagtg ccacaaacaa aaccatggga gctattgtcg gaattatcta ttcctgagca
     2221 cagttatttt gtgagaaaaa cccatgcgaa ttcattttat aagactaatt tgagtgtttt
     2281 attaactgaa ttggactgtc attcgatcga attatgtggg gcacaagcag agtattgtat
     2341 agatacgact gtgaagtttg ctcatggatt aggataccag cttatgatgt gtagaggagc
     2401 aacgacaact tatgataatc cttttatgaa agcaaaggaa acaatcgctt tttacgagaa
     2461 aatttgggat aaacgatttc tgacattttt ggagcaataa gttgtttctt cgtttaaaaa
     2521 aagcagaatg gatactggtt tatccattct gctttattac gtaaaatgag aaaataatcc
     2581 tgttctattt ttatgggata aaccatttat tttttgtata aaaaatgttt gcgtttacat
     2641 ataattctcc tagaaacata aaaaggagtg aatagcttga gaacaaaaca agtattaaag
     2701 ggagcgttgg tcatcttatt tgctgtaagt acaagcgtcc cgcaatggac agtatatgga
     2761 gagagcattt cttccgaatc ggaatctcgg gaggtagaaa ccacagaaac tactgatgct
     2821 ggaaatgaaa cgaaatcgag tacttcacaa acagaaacaa atactgaaac aacggttgag
     2881 acatctgtta ctagcacaga tcaagaaacg accgctacca cagataacca acaaacagaa
     2941 gaagcaacag ctacaaaaat gtcaaacgac gaatcctctt ctgctaaagt aagtgacaaa
     3001 gagaatggga cagaaaatga tgatatcggt aaaggtgaaa atttcaatgg taaagaatat
     3061 ttgaattact tcggtaaaca gatgacagaa atacttaatg gaccaacaga agacatcaag
     3121 tcaaaattaa tagagttatt agtcactgag cttgctgtta atgattatgg tgtagataaa
     3181 gagattatca aacatctgat tgctacttat ctattgtacg atatcgcttt tgcaaaaaac
     3241 agtgtgttga tgtcgatgct tggaggtaca gatgatggga caacgcctac agaaaacaca
     3301 ggcttctatc caaatacaaa acaacttcat caaactgtac taaacaaaca agatggcaaa
     3361 atgatggatg tatatgcata ttatgtcgat caaggttcag ataaaacagt catgatccat
     3421 ggcggatttc gagggaattg gaataacggg atcgttacag aagaatacaa tgatttttat
     3481 aaggccgggt ataatttatt atttgttgat tccagagcga ctggtaatag cggaggcgat
     3541 tatgtgacct atggacaata tgagtcagat gacgtccttt actggataaa tcaagaagtt
     3601 cgagagcggc catctcaaaa gattttactt tatggtggtt cgatgggagc agctacgatg
     3661 atgtctgtat tagcgaaaga tattcctgtc aatgtcaaag gaattattga aaattgcgga
     3721 tttgcatcaa ttgatgaaca actacgtttt acgtacagcc agacagtagc ccccgcacta
     3781 ccggatgcca taaaaaatca gttagacatc attggggatc aagagcatga agatttattt
     3841 atgggccttt tgaaacaata ctattttgat caggagatgc atttggatac aaaagctgct
     3901 ctacctacga ttggaatgtc agattcattg cctaaattga ttatccatgg aacagcagat
     3961 gatgtagtac ctgtgtctaa cgcgcaaaaa ttatatgagt tatcaggagg gtataaagat
     4021 cttttattag tagaaggagc agggcatgga aaggcacagg aagttgacca tgctgcctac
     4081 acgaaacatg taacggactt tttgaaagta gtgttccatg atcagataaa cgtaaagtat
     4141 gtggatgaaa acaataaaag tctactcaaa gaccaggacg aaatccagct ttacggagct
     4201 tatggagaaa attatatgac agagcaaaag acattcgaag gatacgaatt agcaaatgtt
     4261 gaaggaccga ctgaagggat ttttaatgag acgataccaa ctattatttt ctattacaaa
     4321 aaaatcccag tagctccacc aaaaaagcaa gatccgacag agaatgcaga caataaggga
     4381 aagaatgaac ctcgagagga tcaatcaagt gatgatacgc aaccgattca gatgacagtg
     4441 aaagtacaaa aaaagacaga tgcaaaaaaa agaatgttgc cgaaaactgg agaaaagaaa
     4501 cattcgcttc tcatttattc aggtggattt ttatctgtta gcacgattat cacatggatt
     4561 tggcgaagaa agagagggta aatgaatgaa atggttcaag cgaatagtga ttgcagtacc
     4621 agttatcatc gtagttattt tagtcactgt atttttgatt aatcaaatca caccaaagcc
     4681 gattagcttg cttgttagaa aacaatttga cacaagtgaa aaagaacaag tatatgcccg
     4741 accagatgat ttccagcaaa aaatcgaaca aatcgatata aaaaaagaaa tatcctatcc
     4801 ttcaaagtac aaaaataata atatggatat ctacacaccg aaagtggaaa agaaaaaatt
     4861 accgattctt ttctggatgc atggaggcgc atatgtagga ggggataaaa atgattgtcg
     4921 tgattatttg gaatatctat gttcagacac gcagcaaatc atcgtaaata tagattatga
     4981 acgatcaccg gaagcaaaac atcctacacc agttatccaa ttgaacgaag ccattcaagc
     5041 agctaaaaaa gagataaaag atcaagcaga ttggtcgaat atttcgattg gtggaaattc
     5101 ggcaggtgca caaattagtg gagaatattt gttagcactt caaaatgaag aaattaaaaa
     5161 agctgatcat cttgatccga ctttggagaa caagcagatt acgaagttta tttctttgtc
     5221 tggtctgttg gatccttctg cacttactca agtaagtgat aaaatctcta gctttcttta
     5281 tgaaaaatgt ggatgggctt actttgataa taaggacttt gaacaatcag agaaggtcaa
     5341 aagtttagcc ttggtcagac atgcggatcg ttggacgcaa aacacattct taacggatgg
     5401 aaatacaaat acgtttacga aacaaatgga agaagtaact tctgtatttg agaaagcggg
     5461 ggtgaaagtc acaaaagtag attatgcgga gaaaaatgct gttttgaacc atgaatatca
     5521 gtttgatttc agcaataaac aagcgcaaga aacatatcaa aagttagtaa tgtttttcaa
     5581 ggagtaaagt cttatgatgc aaaagaggtt gtaatctgtc acttagctcc aagtcacaag
     5641 gagcaatttc aataaacaag cttttcatgg tttatgaata ttgtaacttg tgactgagga
     5701 gttagagatg gcaaggggca ccttttattc gaaatttggg aatatcacac gattattgtc
     5761 acgatctctt ttacgtaaat tttataagtc agatttgttg ttttgatcgt ttaaaaaaag
     5821 caacaaatcc gaagcctgcg gaggcaagaa gggacaaaaa gccgagtggt aagcaaaaga
     5881 atgctggttc cactaatatt ccgttgttat tgatataagg ctgggaacaa agatgaatag
     5941 caaataaaat caatgcacta gcgaaaaaaa gcaaagaaat atggatagct ttcataagca
     6001 gcacctcctt ttttataatt gtatgataaa tagattaaga atctataaat gtgacaagtt
     6061 tgttaggatg tgaataaaat gatcatctag cgaacttgaa acaaagatgg ctaatgataa
     6121 gcaattaatg attcagcaat cagataggta caaagtttac catcatacgt ctagaccaat
     6181 aatagaaaat atgggaatat aatagaaaag atttaggata cccccaaaag aatttcccca
     6241 aaaattcgat gtatcctaaa cttttttatt ttacataaca atcatatcaa ctgtgaaata
     6301 aaattacaaa tgataaccta aaatga
//
LOCUS       DAZKLD010000144         6314 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.145, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000144 DAZKLD010000000
VERSION     DAZKLD010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6314
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.145"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(89..1219)
                     /locus_tag="ACG8KR_002504"
     CDS             complement(89..1219)
                     /locus_tag="ACG8KR_002504"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HFQ0201820.1"
                     /translation="MSKNRPFTVDHVGSFLRPQSIKEARKKVQEGLLSKEELRAIEDQ
                     EIKKLVEDQKAVGIKGITDGEFRRGFWHLDFLEELNGIEGYVPETGYNQTFHGKAAPA
                     YNIRVVDKLSFNPNHPFLKDFAFLKEVVETDDTFVAKATIPSPTMILRQEILSNDGSS
                     KIKEIYPDLSDFYEDLSSTYKAAIHAFYEKGCRYLQFDDTNWAFLADQTKREALLQKG
                     IDPKEMAHICTEIINNALEDKPEDLTITTHICRGNHASSWLFSGGYEPIAEELFSTNY
                     DGFFLEYDSDRAGDFTPLRHWSNKESKVVLGLVTSKFPDLEDTEKIKERIKEAQQFVP
                     LENLSISPQCGFASTEEGNHLTEEEQWKKIQLLQQVAKEVWL"
     misc_binding    complement(1279..1506)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(1868..3070)
                     /locus_tag="ACG8KR_002505"
     CDS             complement(1868..3070)
                     /locus_tag="ACG8KR_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFQ0201821.1"
                     /translation="MPKIKNIYQDKKTKKWFFRAYLGIDENGRKVQKTKRGYASQKDA
                     KIAYDKYMETHDFNKTIPNQLCSTNQMTFEEFYKDRFVKWYERQVKSQTYENAQFIFE
                     KRMQFFYHFRVRDITSHDIEDWLFELSQTESRNSRKKVNGETLSKSYINRIRGHLKIV
                     LDRAVKEGVIAKNPVEDVSSLPSENKKVEFWEHSEFQKVMNELKDDKIQTHHRKIVYE
                     MLFYTGIRIGELEALSWSNVDFEKNTITIEKTLIYNTKDDWYFSTPKTKCAYRTIGLG
                     KKLSLKLRQWYDLQKRIGNFEYVAQLDGTFTPPYSFANWLKEAAKKAGVKPIKLHALR
                     HSHVAFLIEQNIQPLSIQERLGHANIQITLGTYGHLYAKSDQQVVSAIDNVQEGAILS
                     KNDNLLPV"
     gene            complement(3130..3333)
                     /locus_tag="ACG8KR_002506"
     CDS             complement(3130..3333)
                     /locus_tag="ACG8KR_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HFQ0201822.1"
                     /translation="METKCDFMVNRAILIEMGFKPSQAARMIKESKAYLARIEGIDFY
                     NNRQVGVVPSRVIEHLFHIQVAE"
     gene            complement(3475..4539)
                     /locus_tag="ACG8KR_002507"
     CDS             complement(3475..4539)
                     /locus_tag="ACG8KR_002507"
                     /inference="COORDINATES: protein motif:HMM:NF014537.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFQ0201823.1"
                     /translation="MDSLQEEVVPVNRVLIDYLTIHFFSNQHLKLIEEIIEMPIERFE
                     ELDYAPKGYIGQYVWNQVITIRYSIDDAVKGTVMEFSGQGCKHLARWLKTRKTNWQQF
                     FRKVLDYQGNFTRIDFTLDDFVGSLSIPELKHKVILGHVWTTFQVSESHGGTDIINNE
                     SNGETLYLGSKKSQCRFCFYQKDHEQRKRRGIPLEEAKVKNRFELRYRKEKAQSLAKI
                     ISKTHDLTKLFFELLNGAICFYDRDPNDLGAKVDAKWAAFISNYGAITISLDTIPQSF
                     EKTMSWLIHGVSPSLVFIAQVDQTFHSSLLPLIIEQGEMNPRQIKLLQNMKADPEYYQ
                     EEVEYYERKLQSISNEKTSY"
     gene            complement(4600..4707)
                     /locus_tag="ACG8KR_002508"
     CDS             complement(4600..4707)
                     /locus_tag="ACG8KR_002508"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0201824.1"
                     /translation="MNQVNQHDLGEAIRVSREERGWTQRYLAEKVGISR"
     gene            4870..5682
                     /locus_tag="ACG8KR_002509"
     CDS             4870..5682
                     /locus_tag="ACG8KR_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0201825.1"
                     /translation="MEIDKQAVGNRIRQIRQELKLSMEKFGKLIGDLPRSTVNNWERG
                     INLPKTETLHQIAEIGHVTNEYLLYGDQENQYILEMLQKKAGKLDPKIEGLIVDEIKH
                     ADLVSEKEMDRMIEFFITNLIPPTEQDQFTFQCIDEKKQLYLGSTNFGKEAQLYLQHD
                     NQNHILHMMPFTFSNFSVDRILVYLANQKSYSYFGKHLPKELGEKAILLYSINQLTSD
                     VRIAPLVYSEETASYQYTEDNQYLLEQQYLYLPFVMEVEKERLLNAAYPSSK"
     gene            complement(6056..6247)
                     /locus_tag="ACG8KR_002510"
     CDS             complement(6056..6247)
                     /locus_tag="ACG8KR_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0201826.1"
                     /translation="MKQKFFIAIIATKKFHRGKRFGQKWKFPPKVTAPQLKSIKELEF
                     ENALVICMTCTDEVFRKHF"
BASE COUNT         1873 a         1299 c          997 g         2145 t
ORIGIN      
        1 tataaaaggt aaataaaaaa gagtggattt ctccactcca tgaaaagccg actttttggg
       61 acgctttttt ttgccaagcc tcacattttt atagccaaac ttcttttgcg acttgctgca
      121 ataattggat tttcttccat tgttcttctt ctgttaaatg gtttccttct tctgttgaag
      181 caaacccaca ttgaggactg atacttagat tttccaatgg tacaaattgt tgtgcttctt
      241 ttattcgttc ttttattttc tccgtatctt ccaaatctgg aaacttagaa gtaactaatc
      301 ctaaaacaac tttactttct ttgttactcc aatgtcttaa aggagtaaaa tctcctgctc
      361 ggtcagaatc gtattccaaa aagaaaccat cataatttgt tgaaaacaac tcttctgcaa
      421 tgggttcata tccaccggaa aacagccaag aagaagcgtg attcccgcgg cagatatgtg
      481 ttgtgatcgt caaatcttct ggtttgtctt ccagtgcgtt attgataatc tctgtacaaa
      541 tatgggccat ttctttcgga tcaattcctt tttgaagaag tgcttctctt ttagtttgat
      601 cagctagaaa cgcccaattt gtatcatcga attgcaggta acggcaacct ttttcataaa
      661 aagcatgaat cgctgctttg taagtagagg acaagtcctc ataaaaatca gatagatcag
      721 gataaatctc cttgattttt gaagaaccat cattagatag gatctcttgt cgtaaaatca
      781 tcgtgggact agggatcgtt gcttttgcaa caaaagtatc atctgtttcc actacttctt
      841 tcagaaaagc aaaatctttt aaaaatgggt gatttggatt gaaagataat ttatctacta
      901 cccgaatatt ataagctggt gctgcttttc cgtggaatgt ttgattatat ccagtttctg
      961 gaacatagcc ttcgatacca tttagttctt ctaaaaaatc aagatgccaa aatccacgcc
     1021 gaaattcacc atctgtaatt cctttgatgc cgactgcttt ttggtcttcc actaattttt
     1081 ttatttcctg gtcttcgatt gcacgaagtt cttcttttga aagtaagcct tcttgaactt
     1141 tttttcttgc ttctttgatc gattgcgggc gtaagaaact tccgacatga tctactgtaa
     1201 atggtctatt tttgctcatt tcactcactc ttttctttgt tttttgtaaa aaaaaatcct
     1261 tttactagca aatactagta aaaggacgat tatactcgtg gtaccacctt ttttcagaaa
     1321 aaacattctt tctctcagcc tgacaataaa atcagtggtc ttggtaacga aagactgttc
     1381 gtttttgcct acctttcgac aaaaaatcct ctaaggccat tttcgacagt caacttctag
     1441 ctttttacac caaccaaagc ctctcttgaa ccattttctg tctactttcc ttctcatcgg
     1501 atactattag aataaaatta gaatagcatg tttgaaagaa aagtcaatag ggaataagaa
     1561 caaacaaaaa ctaatttgtt attcttagta atttgtttgt ggaatacaat aaagcttttc
     1621 taatatgata attgttcagt cgtcctttca tcttagatca cttccttaaa atgatcatac
     1681 ccgtttcaac cacataaaaa aggacttaat tttgaaaaat ttcccatcaa aactaagtcc
     1741 tttttattat agttatgcaa atttgcttat tttctcgcta tttccgatac atcttgaaca
     1801 ggagcaattc gactgaaaac actggtgcaa aagcatttat ttgtcttatg gtagaaccgt
     1861 ggaaaactca gactggtaat aaattgtcat tttttgaaag aatagcgccc tcttgaacat
     1921 tatcaatcgc acttaccact tgttgatctg actttgcata taagtgaccg taagttccta
     1981 aagtgatttg aatattcgca tggcccaatc gttcttgaat agatagcggt tgaatattct
     2041 gctcaattag aaatgcaaca tgagaatgtc gtagcgcgtg aagcttaatc ggtttgacac
     2101 ccgctttttt agcagcttct tttaaccagt tcgcaaaaga ataaggtggc gtaaatgttc
     2161 cgtccaactg agcaacatat tcaaaatttc caatacgttt ttgcaagtca taccactgtc
     2221 tgagttttaa ggaaagcttc ttccccaacc caatcgttcg ataagcacat ttagttttgg
     2281 gagtcgaaaa gtaccaatca tcttttgtat tataaatcaa tgtcttctca atcgttatcg
     2341 tgtttttctc aaaatctaca ttcgaccaag acaaagcttc aagttctcca atccgaatgc
     2401 cagtataaaa gagcatttca tacacaatct tccgatggtg ggtttgaatc ttatcatcct
     2461 tcaattcatt catcactttt tgaaactcac tgtgttccca aaactcaact ttcttattct
     2521 cagaaggaag cgaagaaaca tcttcaacgg gatttttagc aatcaccccc tctttaacag
     2581 cccgatccaa tacaattttt aggtgccctc gaatccgatt gatataagat tttgataacg
     2641 tttcgccatt aacttttttc cgtgaattac gtgactctgt ctggcttagt tcaaataacc
     2701 aatcctcaat atcgtgactt gtaatatctc gaacacgaaa atgatagaaa aactgcatac
     2761 gcttttcgaa aatgaattgc gcattctcat atgtttgact tttgacctgt ctctcgtacc
     2821 attttacaaa cctatccttg taaaactctt cgaaagtcat ttgattggta gaacacagtt
     2881 gattaggaat tgtcttgtta aaatcatgtg tttccatgta cttgtcataa gcaattttgg
     2941 catctttttg tgaagcataa cctcgtttag tcttctgaac ctttcttcca ttttcatcta
     3001 tacctagata cgctcggaag aaccattttt ttgtcttttt atcttggtaa atatttttaa
     3061 tttttggcat cgttccattc ctccctgtac ttgagaaaag ctactttaat agtaccgcgc
     3121 ttgtactgtt tattccgcaa cttgaatgtg aaaaagatgt tcaattactc gggatggcac
     3181 cacaccaact tggcgattat tataaaaatc aataccttca atccttgcaa gatatgcctt
     3241 gctctcttta atcattcgtg cagcttgact aggtttaaag cccatctcta tcaaaattgc
     3301 acgatttacc atgaaatcac attttgtttc cattctctca cctcctgatt ctttactcct
     3361 tttaaaataa tcctctttat aatctattgt ttctcattcg atggttgtgt tatactcgtc
     3421 ttgtcgaggc ggagtacgtt gcacaacttg ttgtgtaagc agtactttgc ttttttagta
     3481 gcttgttttc tcatttgata tactttgtaa ttttcgttca taatactcaa cctcctcctg
     3541 ataatactcc gggtctgcct tcatattttg gagaagtttg atctgtcttg gattcatttc
     3601 tccttgttca ataatcaaag gcaacaagga agaatgaaac gtttgatcga cctgtgcaat
     3661 aaaaactaat gatggtgaaa caccatgaat taaccaactc atagttttct caaaactttg
     3721 aggaattgta tctaaagaaa tggtgattgc gccataattg ctaataaagg ctgcccattt
     3781 ggcatcaact tttgcaccta gatcatttgg atcacggtca taaaaacaaa ttgctccatt
     3841 aagtaactca aagaacagtt tggttaaatc atgggttttt gaaataatct ttgccaagct
     3901 ttgcgctttc tcttttcgat accgtagttc aaagcgattc tttacttttg cctcttctaa
     3961 aggaattcct cgtcgcttcc gttgttcatg gtccttctga taaaagcaaa aacgacactg
     4021 actcttcttt gagcctaaat acaaggtctc accatttgat tcattgttta tgatatccgt
     4081 accgccatga gattcactca cttgaaaagt ggtccacaca tggcctagta ttaccttatg
     4141 cttaagttct ggaatgctga gactccccac aaaatcatct aaagtaaaat caatccgtgt
     4201 aaagttcccc tgataatcta ataccttacg aaaaaactgt tgccaatttg tttttcgtgt
     4261 ttttagccat cttgctaagt gtttacagcc ttgacctgaa aactccatca cagttccttt
     4321 aaccgcatcg tcaatggaat accgaatcgt gattacttga ttccacacgt attggccaat
     4381 atagcccttt ggcgcataat ccagttcctc aaaacgttca atgggcattt caataatctc
     4441 ttcaattagc tttaaatgtt gattcgagaa aaagtgaatg gttagataat caatcaaaac
     4501 acggttaact ggaacgacct cttcttgcaa actatccaac acaagattca atttttcttc
     4561 acttaattgt ctcgttcctt tttctacttt cgataataac tatctcgaaa tccccacttt
     4621 ttctgctaaa tatcgttgtg tccaccccct ttcttctcga gaaactcgaa tggcttcacc
     4681 gagatcatgt tgattaactt ggttcatcga caaacctcct tctgctattt acttgtcaaa
     4741 gaacctattg aacttatttt aagtacattt ttattgtacg cctgtattta ttttaagtca
     4801 atatgttttg acttttttta agtacacacg tatactaaat ttaagaactt atcctgagga
     4861 ggagttttca tggagataga caagcaagca gtcggcaatc gaattcggca gattcgccaa
     4921 gaattaaagt tgagtatgga gaaatttggc aaactaattg gtgatttacc gcgaagtaca
     4981 gtcaataact gggaacgtgg gatcaacctt ccaaaaaccg aaacccttca ccaaatcgca
     5041 gagataggtc atgtcaccaa tgaatatctt ttgtatggtg atcaagagaa tcaatacatt
     5101 ttagaaatgc tccaaaaaaa ggcaggtaag cttgatccta agattgaagg tctcatagta
     5161 gacgaaatca aacacgctga ccttgttagt gaaaaagaaa tggatcgaat gattgaattt
     5221 tttattacaa atctcattcc accaactgaa caagaccagt ttactttcca atgtatcgat
     5281 gaaaaaaagc agctttatct tggttcaact aattttggta aggaagccca gctctatttg
     5341 caacatgata atcaaaatca tattctgcac atgatgccct ttaccttctc caactttagt
     5401 gtggatcgca tactcgtcta tttagcaaat caaaaatcct attcctattt tgggaaacat
     5461 ttacccaagg aactaggtga gaaagccatc cttctatact caattaatca attgaccagc
     5521 gatgtgcgaa ttgccccgtt ggtctattca gaagaaaccg cttcttatca gtacacagag
     5581 gacaatcaat atctactaga acagcaatat ctttatctac cattcgtgat ggaagtcgag
     5641 aaagaacgcc tgttaaatgc agcgtatcca tcatcaaaat gaggtgacaa aattgataag
     5701 cacgtgacaa agaaaatact gctaggacac tccccaatgg gtagttgtca cagtttcctg
     5761 ttctttgtta gccccctgtt agaaccgggg gctttattcc tgcagtccgg ctacgccttc
     5821 ctgcaataga caaaactcca gatcgggtaa ttcgcttgtg gcgaaacccg acctggagtt
     5881 tgtctctgta ttttgaaccc taaaaattaa tcaaacagac tgatattctc ataatcagcg
     5941 cgaaataagc cattaagtac agttttccta cctatttttg ataacccgct ggttagttaa
     6001 tattcctttt ccgagagaga taaatgattg gttaaatcta tatacatctc aacctttaaa
     6061 aatgttttcg aaatacttca tcagtacatg tcatacaaat taccagtgca ttctcaaatt
     6121 ctagttcttt aattgatttc agttgaggag cagttacttt tggcggaaat ttccattttt
     6181 gtccaaacct cttcccccga tgaaatttct ttgttgcaat tattgcaatg aaaaattttt
     6241 gtttcaaaaa tatacataat tacttcctcc aataaaaact atctacctct caaattattt
     6301 ttctctaata agaa
//
LOCUS       DAZKLD010000145         6303 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.146, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000145 DAZKLD010000000
VERSION     DAZKLD010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6303
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.146"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            334..714
                     /locus_tag="ACG8KR_002511"
     CDS             334..714
                     /locus_tag="ACG8KR_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HFQ0201827.1"
                     /translation="MSEQHQNLASDHSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVAPD
                     DAQRLSENLKIMQKELQKIWQQLK"
     gene            696..1610
                     /locus_tag="ACG8KR_002512"
     CDS             696..1610
                     /locus_tag="ACG8KR_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HFQ0201828.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIR
                     TLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPNHQIAIYTHTDKDHIHN
                     HIVINSVNLETGKKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKTEKRTMSEIKL
                     KEKNEPVWKDEIRFAIDQTMKNKKTRTYTQFCDSLKTFGIHCLNRGKNFTYELVHKKK
                     KVRSNKLGKDYEKETILHELDRRERTYNQQRTNELRRARASIRETAEEVRREQRTFTP
                     EQSEIRPTRRNHEQHVERGTDHQQELSL"
     gene            1855..2544
                     /locus_tag="ACG8KR_002513"
     CDS             1855..2544
                     /locus_tag="ACG8KR_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0201829.1"
                     /translation="MAFYLPYLLISASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLV
                     GVIFALSIVLSGLFGVLEGNKRVSRSILLGDITQKYEAACDKKKKEQALAQKMEEFTA
                     CYEDMNNIFVDQEKRLTDKNMKKLTRFYQNLPEKQQKEVQEKYEQVKKDVQYFKDTQT
                     EEACCNLFSDTIPFSTSEQERKERQQSVTYERYETLLQQATNIQNPTKKETALNYLES
                     VKEWLDQQQQN"
     gene            complement(2665..2751)
                     /locus_tag="ACG8KR_002514"
     CDS             complement(2665..2751)
                     /locus_tag="ACG8KR_002514"
                     /inference="COORDINATES: protein motif:HMM:NF033608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HFQ0201830.1"
                     /translation="MFERFICPLLVEVCVSLFSYWLNNRNKK"
     gene            3427..4377
                     /locus_tag="ACG8KR_002515"
     CDS             3427..4377
                     /locus_tag="ACG8KR_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HFQ0201831.1"
                     /translation="MANEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMREKGDKTVR
                     LTFDQLKDLSNYKATANNRFINDLEMTYDKLMDLRFGKRSANGLDIDRFVMFSEFKIR
                     GTAKDPYVDIQVYEKALPLLNNLDEWVRYSLQQFNELQSSYAKTMFRLLKQYRTKGFA
                     YFSKEDFHELLDIPKSYWNNQANVDNRVIKPIRQELTAIFKGLSIKKKYGKGRGKPVV
                     GYQFTFKPEIKKADDFNKHAPTKEEQNKALALADKQKEYEEPATTINEKIKQKKRELE
                     LLQQIKQLEKEKEALEKQTESALSKKEKKSLLSDLDNLFQ"
     gene            4398..4931
                     /locus_tag="ACG8KR_002516"
     CDS             4398..4931
                     /locus_tag="ACG8KR_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HFQ0201832.1"
                     /translation="MQPQETFTIKELSELFKMSRQAISKHIQKLDSSMIAKNERGYKV
                     VLRSGVLQLARNLGNQMLLDRLHEYEQDNDKNVNIPSKNVVTNKLLDQLREENEFLRE
                     QLALREQNNSHIQKLLDQQQQLTLQANKQIEKLQEKLQLTYTEEAPENDSTTLSEATE
                     TIDKQIDTEKKWWQLWK"
     gene            4947..5117
                     /locus_tag="ACG8KR_002517"
     CDS             4947..5117
                     /locus_tag="ACG8KR_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0201833.1"
                     /translation="MTRKNLLFGSILLGILGFSPMLLSVINKGNKHGELKLNPNYYNS
                     PNEELFVKETDK"
     gene            5567..6100
                     /locus_tag="ACG8KR_002518"
     CDS             5567..6100
                     /locus_tag="ACG8KR_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0201834.1"
                     /translation="MKKFTLGVALLTIGAVVLPSTTIVYASENSQVVSLNENNATENV
                     VIEYTEDGMVVTKYEDGYSAEDFANSSDIMTRAAVARWGKKEYTNIAVTTGVAANAIN
                     AAFWAGGKASTSMFGIPGWAIGALLTGASWTKKGASPGNAVAKEWDKNGNGWIGFYYQ
                     RGYDAAGRVVATRYSTL"
     gene            6112..>6303
                     /locus_tag="ACG8KR_002519"
     CDS             6112..>6303
                     /locus_tag="ACG8KR_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0201835.1"
                     /translation="MFLISKSLKSLKTFTKKNPQIATIRIFIFAIAVAIIVNMLSGGQ
                     LYKSGLFLVLILVVLDIIKS"
BASE COUNT         2323 a         1102 c         1174 g         1704 t
ORIGIN      
        1 aaaaaaaaat aaatcgatac attttagaag aatagggttg tttgaatatt acaaagcaac
       61 aatttcacct tcggtgaaat aagcgaaact ttacaaaaag tggctagcaa atgctagcct
      121 ttttttgtat tgtggagcgt ggattttgag ggggattcaa gggggagctt gcgctccttt
      181 gacaagcggt tatttgcacc aaatacggag gtatttggct agcaaatata gagcttgcca
      241 aaccctttta gtgagcttta ctcattgtac ctttttaggt acaatggtat tgcgaacgat
      301 acgtccgtag gacgtactga aagggtggga cttatgagcg aacaacatca aaatttggct
      361 agcgatcatt ctaaaaaata tacctatcga tccgagccaa aacaaatcag ttttcgagtg
      421 agcgaatccg aatttgcaaa gctgaagcag tcagctgaag ctttgcaaat gagtgtgccg
      481 gctttcgtga aagccaaggc acaaaacgcg cgtctggtga caccgaaagt ggcgccagac
      541 attgcccagg caatcgcgcg cgatttagcc aaagctggag ggaatatcaa tcaaattgcc
      601 aagtggtgca atacacacca acacgacgtt gctcctgatg atgctcaacg tttatccgaa
      661 aacctaaaaa tcatgcaaaa ggagctacaa aagatatggc aacagttaaa gtaagtcgaa
      721 ccacttcatg tagccgagca atcaattacg cagaaccacg agcaaccgtc aaaacaggaa
      781 taaactgtga cattgattat gccaaaagtg aaatgaaaca aatacgcacg ctttatggaa
      841 aagacgatcg tgtacaagca cacttattga ttcagtcctt tcgaccagga gaaattaccg
      901 ctgaaaaagc caatcaacta ggggcagagt atgcgaaaaa aatagcccct aaccaccaaa
      961 tcgcgattta cacacataca gacaaagacc atattcataa tcacattgtc attaactccg
     1021 ttaatcttga aactgggaaa aaatttcaag cccatgggca agcattttta aacaaatgtt
     1081 atgacctaaa tgacgagatt tgtcttgaac atgacttaac aatcacggaa cgaggaaaga
     1141 aaaccgaaaa acgaaccatg tctgaaatta aattaaaaga aaaaaatgaa ccggtttgga
     1201 aagacgaaat tcggtttgcg attgatcaaa caatgaaaaa caaaaaaact cgaacgtata
     1261 cccaattttg tgattcactt aaaacattcg gcattcattg cttgaatcgc ggaaaaaatt
     1321 ttacttacga attggtccac aaaaagaaaa aagtacgatc caacaaacta ggaaaggatt
     1381 acgaaaagga gacgatttta catgagctgg acagacgaga acgaacctac aatcaacaac
     1441 gaaccaacga gctacgaaga gctcgagcta gcattcgcga aactgcagaa gaagtacgaa
     1501 gagaacaacg aacttttacg cctgaacaat cggaaattag accgactcga agaaaccatg
     1561 aacaacacgt tgaacgagga acagaccacc aacaagaatt atcgctctaa aacgttaaaa
     1621 gacatcgaaa acgaaataaa gagacaacgt gcctttcttg cgccttatac ccaagaaagg
     1681 atacaaacta cgatggattc acaagtcaaa aaactccacc aagcgctatt agaccagcga
     1741 aaccaagaac aaaaaaaggc tttaattcaa cgatcgatgg atattcttgc ccccttatgt
     1801 tgcttagtac taacattatt agccttttgg ttggtatggg gagaataaac agacatggca
     1861 ttctatttac cctatctact catttctgcc agtattttag gaattatttg gcttagctat
     1921 aaggcttatc aaacaagata ttcattaaaa ggctctaaaa ttcgatttaa gcgattttta
     1981 ttggtcgggg ttatatttgc cttatcaatc gttttaagtg gtttattcgg cgttttagag
     2041 ggtaataaac gtgtttcaag aagcatttta ttaggggata tcactcaaaa gtatgaagca
     2101 gcatgtgata aaaagaaaaa agagcaagca ttagcacaaa aaatggagga atttaccgct
     2161 tgttatgagg acatgaacaa tatttttgtg gatcaagaga aacgtttaac agataaaaac
     2221 atgaaaaaac tcactcgttt ttatcaaaac ttaccagaaa aacagcaaaa agaggtacaa
     2281 gaaaaatatg agcaagtaaa aaaagacgtg caatatttta aagatacgca gacagaagaa
     2341 gcttgttgta acttatttag cgatacaatc cctttttcaa ccagtgaaca agaacgaaaa
     2401 gaacggcaac aatcggtcac atacgaacgt tatgaaacac tgcttcagca agcaacaaac
     2461 atacaaaatc caacgaaaaa agaaacggca ttaaattatc tagaaagtgt caaggaatgg
     2521 ttggatcagc aacaacaaaa ttgaatttga ttagcaatca tgttaaactg aacctgcaaa
     2581 tgatcaaaca ttagatacac gaaagcccca gagaggtcgg attctctggg gctttttttc
     2641 aggttgatgt cgcctttatt gattttactt cttgtttcgg ttattcaacc aataactgaa
     2701 taaagagaca caaacttcta caagaagggg gcaaatgaac cgttcaaaca ttagatacac
     2761 ctccttccag tcgcaagact gtcggatagg cgacaagtta ttatacctta caaaataaaa
     2821 tctgtgtggt gtttttaagt atcccttatt ttcgggttgt tagggcttgc cctgaccgcc
     2881 tgtaaggcgc tttaagaaca agataaggtt ttttacccaa gactgtctta aaacgactta
     2941 gacgagctat cagaggcttc ccccacctcc gttactgact tgtttttagc aaaggagcct
     3001 ttaggcgact gcgcagtaca atgtgagagc atagcgatca cactttttta gcgaccgtag
     3061 ggagcaaggt tttgaattgg cagttttgaa aaatgcgcga acctgttggt agaaaaaaga
     3121 agtggttttt aggcgggaaa ttttgtcacc ctcttttata atctcttttt aaaccttttt
     3181 ttaaacctct ttaccgtcgg ctgtgtcctt actccttcaa tggatacagc gttttataaa
     3241 ctacattttg tctgctttta aactacattt tgtctgcttt taaactacat tttgtctgct
     3301 tttaaactac attttgtctg cttttaaact ctttttttgc actcacgtat gttataatat
     3361 gcattatcac agacaaaacg ttgtttataa cgtctgtatc cattgaagga gtaaggagga
     3421 atatctttgg caaatgaaat cgtgaaatac catcatgaat taaataccat tccgttgcgg
     3481 aaatttacac cagttgaaat gaatctattt ttctctattg tgtctagaat gcgcgaaaaa
     3541 ggcgataaaa cagtccgtct tacttttgac caactaaaag atttaagtaa ttataaagca
     3601 actgctaata atcgtttcat taatgattta gaaatgactt atgataaatt aatggattta
     3661 cgttttggta aaagaagtgc taatgggctc gatatagata gattcgttat gtttagtgag
     3721 tttaaaatac gtggaactgc taaagatcca tatgtagaca tacaagttta cgaaaaagct
     3781 ttacctttac taaacaattt agatgagtgg gttcgctatt cactccaaca atttaacgaa
     3841 ttacaaagta gctatgcaaa gactatgttt cgattactaa aacaatatcg aactaaaggt
     3901 tttgcttatt tttctaaaga ggattttcat gaactattag atattccaaa aagttactgg
     3961 aacaaccaag caaacgttga taatcgtgtt ataaagccta tacgacagga attaaccgca
     4021 atcttcaaag gattatcaat caaaaagaaa tacggaaaag ggcgaggaaa acctgtcgtt
     4081 ggctatcaat ttacgtttaa accagaaatc aaaaaagcag atgattttaa taaacatgca
     4141 ccaaccaaag aagaacaaaa taaagcattg gctttagctg ataaacaaaa agaatacgaa
     4201 gaacctgcta caacaattaa cgaaaaaatc aaacagaaga aacgagaact tgaactcctc
     4261 caacaaatca aacaactaga aaaagagaaa gaagctcttg aaaaacaaac agagtccgca
     4321 ctttcgaaaa aagagaaaaa aagtttatta tctgatttag ataatttatt ccagtagcaa
     4381 cctaggaggc aaccagtatg caaccacaag aaacctttac aataaaggaa ttgtcagaac
     4441 tttttaagat gagtaggcaa gcaatatcga aacatatcca aaaattagat tcttccatga
     4501 tagctaaaaa tgagcgtggt tataaggttg tattgcgttc tggagtattg caacttgctc
     4561 gcaacttagg caaccaaatg ttgctagata ggttgcacga gtacgaacaa gacaacgata
     4621 aaaatgttaa tataccaagc aaaaacgtgg ttacaaacaa gttgctagat cagttgcgtg
     4681 aagaaaatga atttttacga gaacaacttg ccctcagaga gcaaaataat tctcatatac
     4741 aaaaactact cgaccagcaa caacaactca ccctacaagc caacaaacaa atcgaaaaac
     4801 tgcaagaaaa actacagttg acttatacag aagaagctcc agagaatgac tcaacgactc
     4861 tttctgaagc aacagaaaca atagacaaac aaattgatac tgaaaaaaaa tggtggcaac
     4921 tttggaaata actggaggga acattcatga caagaaaaaa tcttctattc ggttctattt
     4981 tactaggaat tctaggattc agtccgatgt tattgtcagt gatcaataaa gggaataaac
     5041 atggtgaatt gaaattgaat ccaaattatt acaacagtcc taacgaagaa ttgtttgtaa
     5101 aagaaacaga taaataataa aaaagactgc tctttcgagc agccagttaa aaaataatga
     5161 gtgtttattc ttgaaaaagc aacgcgctta tgaagcgcta ccaaaaattt atcatatttt
     5221 attaaaaata acaacccagt aaaaaatgac tggtaaaaat tcacaaagaa acggaaagaa
     5281 gcaaccgcag aaaatcaagc ttaaaaagag cttttgacct agaccaatcg aaaatcacaa
     5341 agtgagcgcc tgtatctatt gctacaggcg ctacttggca tagtgtacat tatgtcaaca
     5401 agaatttaat ataacatatt ttcctaatgc attctaacta taccacatta tcaaaaatat
     5461 aaattatctt tactaaataa aatattgaca agcctatacc ttttgtttta tgattttcac
     5521 aaagaaaacg ttttctatat atttttctgt atcaggagga aattctatga aaaaatttac
     5581 tttaggggta gctttgttaa ctattggggc agttgtttta ccgtcaacta ctatagttta
     5641 tgcttcagaa aattcccaag tggtatcatt aaacgaaaac aatgctactg aaaatgtagt
     5701 tattgagtac actgaagatg gtatggtcgt aactaaatat gaggatggtt atagtgcgga
     5761 agattttgca aattcttcag atatcatgac aagagctgca gttgcaagat ggggcaaaaa
     5821 ggaatataca aatattgctg taaccactgg agtagctgct aatgctatta atgctgcctt
     5881 ttgggcagga gggaaagcta gcacttctat gtttggtatc ccaggttggg caattggtgc
     5941 tttgttaaca ggtgcaagtt ggactaaaaa aggtgcttct ccagggaatg cagttgcaaa
     6001 agaatgggac aaaaatggta atggctggat tggattttat taccaaaggg gatatgatgc
     6061 agctggaaga gttgtagcta ctagatattc aacgttataa gttaaggagt gatgtttttg
     6121 atatctaaat cattaaaatc attaaaaaca tttactaaaa agaatccgca aatagctact
     6181 attcgtattt ttatatttgc tatagctgtt gctataattg ttaatatgct aagcggaggg
     6241 caattataca aatctggttt atttttagtg ttaatcttag ttgtcttaga cataataaag
     6301 tct
//
LOCUS       DAZKLD010000146         6276 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.147, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000146 DAZKLD010000000
VERSION     DAZKLD010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6276)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6276)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6276
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.147"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..290
                     /locus_tag="ACG8KR_002520"
     CDS             <1..290
                     /locus_tag="ACG8KR_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000751236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0201836.1"
                     /translation="SDEELYKEFGDLSGEQLEESIPGISDSDKIVVLSAGGQLMGDGK
                     SELVSYKQNHRQFIDVNTDSNAISIKDLKELLNSKRNELKQIKETNSVELE"
     gene            304..750
                     /locus_tag="ACG8KR_002521"
     CDS             304..750
                     /locus_tag="ACG8KR_002521"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0201837.1"
                     /translation="MKKIFGVSVGLLMMLTFGNAVYAAEKEVISDPEQYASRYFADFE
                     EEDKVLALPEGGFLKGEAKIVSMDNPDIIQGSYNSETDPASVSVTEAKKIVAEKFKND
                     IETTPIMPRGAFPPDVGTSGHLLVLAGGGSYKSQPFSGAGWREVWV"
     gene            741..989
                     /locus_tag="ACG8KR_002522"
     CDS             741..989
                     /locus_tag="ACG8KR_002522"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0201838.1"
                     /translation="MGVKPADGTGNFLRWKSYIDDGRVGNVNQANATKSGSIQGMALY
                     AREGFKYFAPNGPGWTMNIGIYYTYNPVAGTYYVVQNI"
     gene            1114..3465
                     /locus_tag="ACG8KR_002523"
     CDS             1114..3465
                     /locus_tag="ACG8KR_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase domain-containing protein"
                     /protein_id="HFQ0201839.1"
                     /translation="MDEMVLSTQKWLNKKYSNVTGFDKVPENGRTGWPTIYGLIEGLQ
                     VELGITNLVANFGPTTEKMYDNQVTPKWGKNLPKNIVFLIQGAFWCKGINPGGFDGVY
                     TPYLDTAVKELQTDAGFNGSAVTGVLDAKWAKALFDMSAFVLVPGGDSKIRKMQQWLN
                     VNYLEYTGIMPCDGIYQRNSNQALIFALQAELGYSPSEATGSYGNGTTSKTYPVSEGN
                     SGNYVRIIQYGLYVNGYFEDGTFDGIFTKYMGLEVLAFRKFMVLPEYTEIADVVVIKG
                     LLSSAGDIRRSADGADTSTQLTRSQIQTLVDNDIKIVGRYLTGTVGIGKDERNKYLTT
                     EELNNIFSKNLSVFPIYQDGGAALAYFTYDRGLSDGKKAIDAAKNLNIPLTTVIYFAV
                     DLDMLGEEILAYAGEYFKGVSAVMSYSGYQTGVYGTRNVCSQIINNGYASFAFVSDMS
                     TGYSGNLGFPMPRQWSFDQFVEFTIGSGNGAVGIDLIATSGRDSGFNELSNDTNNDNY
                     IAKYNQKVINQMVRAYQYLSQAGLDNPWNPHLTFYRYVNYSGLSWDIISSPVTEHDRK
                     IYDEYRNKLTNSEGLYNYFIDPNTGSIIGLPHLIVTLQSQMFLADTINDSVSDFAGWL
                     GDLMTCWGEVKKLGIQMEQGVFALVGTAAGGTFSLEDLYQDVDAVNVYTLAKRNSDST
                     ALSALKTYYCDKGAIDRFNQFVKNRFGSSDKIASVTNSLLENDETVDILGVGIISFFI
                     DKIVNNSLGTDFTYLDFIGDEGKVANGFSEKIIQFQENRSKLN"
     gene            3545..3940
                     /locus_tag="ACG8KR_002524"
     CDS             3545..3940
                     /locus_tag="ACG8KR_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0201840.1"
                     /translation="MMIMEEIEWNNLTQNNKNNSGCGIIFMFVIVLGIVVLLITVKIV
                     DYFANTPEEQIQRKLPEFERVPPYDPRDNSFQVKKLKESPNEMWFEVTVESSDFKEKK
                     KEYNYIVKAKVSKRFIFNPKLEIYSVVIE"
     gene            4265..4882
                     /locus_tag="ACG8KR_002525"
     CDS             4265..4882
                     /locus_tag="ACG8KR_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFQ0201841.1"
                     /translation="MRKFFYIRVSSYDQSIERQLISAKELGIPEEYIFVEKASGKDFK
                     RPEYQLMKRMFRKGDLLYLQSLDRLGRNKQMILEEWRELTKEKQIDISVLDMPLLDTA
                     SYKQLDGLETLISDLILQLLSYMAEDERKRINSRQREGVEAAKQKGVKFGRKKIALPP
                     EFPAIYSDWKAGKITAVYAMDQLGMKRNTFYRRVKEYEHTLHHFS"
     gene            4923..5222
                     /locus_tag="ACG8KR_002526"
     CDS             4923..5222
                     /locus_tag="ACG8KR_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BRCT domain-containing protein"
                     /protein_id="HFQ0201842.1"
                     /translation="MDLTNQRVVFTGTLQQMTRTQAIGLVHSLNGHCQSSVTSKTNFL
                     VCGQYSKSLFDDSLYTKKEQTARDLIALGHEITILSEVEFYALISQQLKEWQRYL"
     gene            5256..5423
                     /locus_tag="ACG8KR_002527"
     CDS             5256..5423
                     /locus_tag="ACG8KR_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFQ0201843.1"
                     /translation="MKRKSYDRWGIEITSFNRNIAFHVECDCGELAKSVYQKNYFYCP
                     KCHRRYGVHGT"
     gene            complement(5468..6073)
                     /locus_tag="ACG8KR_002528"
     CDS             complement(5468..6073)
                     /locus_tag="ACG8KR_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFQ0201844.1"
                     /translation="MGKIGYIRVSSQDQNLDRQLAMMEGLNIDKLFQEKASGKDTERP
                     EFQKLLNYIREGDCVIVTSLDRLGRDYEDIKNTVAFMKQKKVALKILDAQFLDFNTGN
                     ELLDTAMFDMFLSLLSYIAQNEREKIRERQRQGVLLAQKAGRYKGRPIEYSINSTDPQ
                     KRLVFKTVVDMLKQGIPVAEIAKENGISRPTIYKIKKANDL"
BASE COUNT         2155 a          874 c         1248 g         1999 t
ORIGIN      
        1 tgtcagatga agaattgtat aaagaatttg gtgatttatc cggggaacag ttagaagaaa
       61 gtattccagg aattagtgac tcagataaaa ttgttgtctt gtctgctggt gggcaattaa
      121 tgggagatgg caaatccgaa ttagttagtt ataaacaaaa tcacagacag tttattgatg
      181 ttaatactga ttccaacgct atttcgatta aggatctaaa agagctttta aatagtaaaa
      241 gaaatgaatt aaagcagata aaagaaacta attcagtgga attagagtaa aggaagattc
      301 attatgaaaa agatttttgg tgtttctgtt gggctactta tgatgttaac atttggaaat
      361 gcagtatatg ctgctgagaa agaagttatt tcagatccag agcagtacgc ctcacgatac
      421 tttgctgatt ttgaagaaga agataaagta cttgcattgc cagaaggtgg ttttttaaaa
      481 ggggaagcta aaattgtatc tatggataat ccagatataa ttcaaggttc atataattca
      541 gaaactgatc ctgcttcagt ttcagtgaca gaagcaaaaa aaattgttgc tgaaaaattc
      601 aaaaatgata ttgaaactac tccaattatg cctcgaggag catttccacc cgatgttggt
      661 actagcggac accttcttgt actagcagga ggaggaagct ataaatcaca gccatttagt
      721 ggtgcgggat ggcgggaggt atgggtgtaa aaccagctga tggtacaggc aactttttac
      781 gttggaaatc ctacattgac gatggaagag taggaaatgt aaatcaagcc aacgcaacta
      841 aatctggttc tattcaagga atggcattgt atgctcgtga aggattcaaa tactttgcac
      901 caaacgggcc tggttggaca atgaatatag gtatttatta cacttataat cctgttgcag
      961 gtacgtatta tgttgttcaa aatatttaat aaaacagcac aaagagattt ctttgtgctg
     1021 ttttattatt tttagttgtt gacaaacact aaaatataaa atataatcaa cacataaacg
     1081 aacgtatgtt cggcaagaaa gcaggggatt tttatggatg agatggtttt aagtactcag
     1141 aaatggctaa acaagaaata cagtaatgtt acagggtttg ataaggttcc agaaaatggg
     1201 cgaacagggt ggcctacaat ctatggactt attgaaggac tacaagtaga acttggtatt
     1261 actaatctag tagctaattt tggacctacc actgaaaaaa tgtacgataa tcaggttact
     1321 cctaaatggg gcaaaaatct acctaaaaat attgtatttc ttattcaagg tgccttttgg
     1381 tgtaagggga ttaatccagg tggcttcgat ggggtatata ctccatattt agatactgct
     1441 gtaaaagagt tgcagactga tgcagggttt aatggaagcg ctgtaactgg agttttggat
     1501 gcgaaatggg ccaaagcgtt atttgatatg agtgcttttg ttcttgttcc cggcggagat
     1561 agtaaaatta gaaaaatgca gcaatggtta aatgtaaatt atttagaata cacgggaata
     1621 atgccttgtg atggtattta tcaaagaaat tcaaatcaag cattaatttt tgcattacaa
     1681 gctgaactag gatattctcc cagtgaagca acagggtctt atggtaatgg gacaactagt
     1741 aaaacttatc ctgtgtctga aggtaactcg ggaaactatg taagaataat tcaatatgga
     1801 ttatatgtta atggttattt tgaagacggt acattcgatg gtattttcac taaatatatg
     1861 ggtttagaag tactggcatt ccgaaaattt atggtattgc ctgaatatac tgaaatagca
     1921 gatgttgttg taataaaagg actattgtct agcgctggag atattagaag atctgcagat
     1981 ggcgcagata cttctacgca attaactaga tctcaaatcc aaacattggt tgataatgat
     2041 ataaaaatag ttggtagata tcttactggt actgttggaa ttggaaaaga tgaacgaaat
     2101 aagtatctaa ctacagagga attgaataat attttttcta aaaatttatc tgtgtttcct
     2161 atctatcaag atggaggtgc agcattagct tattttactt atgatcgtgg cttatcagat
     2221 ggtaagaagg ctatagatgc agcaaaaaat ctaaatatac cattaacaac agttatttat
     2281 tttgctgttg atctagacat gttaggggaa gaaattcttg catatgctgg agaatatttt
     2341 aagggggtat ctgctgtaat gtcatatagc gggtatcaaa cgggtgttta cggaacaaga
     2401 aatgtttgtt ctcaaataat caataacggg tatgcttcgt ttgcatttgt ttcagatatg
     2461 tcgactggtt atagtgggaa tttaggtttt cctatgccta gacaatggtc ttttgatcaa
     2521 tttgttgaat ttaccatagg aagtggcaac ggagctgttg gtatcgattt aatcgctaca
     2581 tcgggtagag atagcggatt taatgagtta tcaaatgata ctaataatga taattacatt
     2641 gccaaataca atcagaaggt tataaatcag atggtacgtg cgtatcaata tctatcacaa
     2701 gctggtcttg ataatccttg gaaccctcat ttgacatttt atagatatgt aaattattct
     2761 ggtttgtcat gggacatcat ttcttctcct gtaacagagc atgatagaaa aatttatgat
     2821 gaatatagga ataaattaac taatagcgag ggcctgtata attattttat agatcctaat
     2881 actggttcaa taataggttt gcctcactta attgtgacgt tacaatcaca gatgtttcta
     2941 gccgacacaa tcaatgatag tgtttctgac tttgcaggtt ggttaggaga tttaatgact
     3001 tgctggggtg aagttaagaa attaggaata caaatggagc aaggtgtatt tgcattagta
     3061 ggtacggctg caggtggaac atttagtcta gaagatttat accaagatgt tgacgcggta
     3121 aatgtttata ccttagcaaa aagaaattct gattcaacag ctttatcggc tttaaaaacg
     3181 tactattgtg ataagggtgc tattgataga tttaatcaat ttgttaagaa cagatttggt
     3241 tcttcagata aaatcgcttc agtaacaaat agtttattag aaaatgacga aacagttgat
     3301 atcttaggtg tagggataat ttcatttttc attgataaaa tagttaataa ttcacttggg
     3361 acagatttta cttatcttga ttttattgga gatgagggga aagttgcaaa tggattttct
     3421 gaaaaaatta ttcaatttca agaaaataga agtaaactta attaagaatc gtttactatt
     3481 taaacagtgt ccttttcctt tttaaatata tacgataaaa taagtatatg aattgtaaaa
     3541 ggacatgatg attatggaag aaatagaatg gaataatctg actcaaaata ataaaaataa
     3601 ttctggatgt ggaattatct ttatgtttgt tatagttttg ggaatagtag tacttttaat
     3661 aactgtcaag atagtagatt actttgcgaa tactcctgaa gaacaaatac agagaaaact
     3721 tcctgagttt gaaagagtac caccttatga tccaagagat aattcatttc aagtaaaaaa
     3781 attaaaagag tctccaaatg aaatgtggtt tgaagttaca gttgaatcaa gcgacttcaa
     3841 agaaaaaaag aaagaataca attatattgt aaaggcaaaa gtatcaaaaa gatttatttt
     3901 taaccctaaa ttagaaatat atagtgttgt aatagaataa tagtgttaaa aaaattgtaa
     3961 aagttaaaga agtcaaattc tgatgtgaaa aataaaatat accatggaag acataaaaga
     4021 aaaactggat aactagttta tttaatggca gaccgttaag gcctgccatt tttatgtatt
     4081 ttagcggaat aaatataatc atgcgggagt gtaaacttac tgtttcaaaa agtatacttt
     4141 ttgacatgcc ttgattttct ttgtttatgt atggcataat aaccacataa tacaatttga
     4201 aacgtttctg atttaatctg attgttttga aacaatttta gtgaaaaaag ggtagataaa
     4261 aacgatgaga aaattttttt atatccgggt cagtagttat gatcaatcga tcgaacgaca
     4321 actgatttcc gcgaaggaat taggaatccc tgaagaatat atatttgtag aaaaagccag
     4381 cggcaaagat tttaaacgcc ctgaatatca attaatgaaa cggatgtttc gaaaaggaga
     4441 tttactctat ctacaatcat tagatagact aggcagaaat aaacaaatga ttttagaaga
     4501 atggcgagaa ttaactaagg aaaagcagat tgatatttct gtgttagaca tgcctctgtt
     4561 agatactgct agttacaagc aactagatgg gttagagacg cttatctctg acttaattct
     4621 gcaattactc agctatatgg ccgaagacga gcggaaacga attaatagcc ggcaaagaga
     4681 aggcgtagaa gctgcgaaac aaaagggtgt aaaattcggc agaaagaaaa ttgctttacc
     4741 tcctgaattt cctgcgatct attctgactg gaaagcagga aagataactg cagtctatgc
     4801 tatggatcaa ttgggcatga aacgaaatac cttttatcga cgggtgaaag aatatgaaca
     4861 taccttgcac cacttttctt gatagatata acagctatta gacgggaagg gagagtttga
     4921 aaatggactt aacgaatcaa agggtcgttt ttactggaac gctgcagcaa atgacacgaa
     4981 cccaagccat cggtttggtt cactctttaa acggccattg tcaatcaagt gttacttcaa
     5041 aaacaaattt cctggtttgt ggtcagtatt ctaagtcgtt gtttgatgat tctctctata
     5101 caaaaaagga acaaaccgcg cgggacttga ttgcgttagg tcatgagatt accattttat
     5161 cagaagtcga attttatgcg ttaattagtc aacagttgaa agaatggcaa cggtatttat
     5221 aatcgttagt aaaatttaca aggaggaaaa tttttatgaa gcgaaaatct tatgatcgtt
     5281 ggggcattga aataactagt tttaatcgaa atattgcttt tcacgtggag tgtgactgtg
     5341 gggaattagc aaaaagtgtt tatcaaaaaa attactttta ctgtcctaaa tgccatcgcc
     5401 gatacggggt acatggaaca tagaaacgaa atagtgcggt agaaaagcga atgaatgtag
     5461 tatatttcta caaatcattc gctttcttta ttttataaat agttggtcga gagatcccgt
     5521 tttcttttgc aatctctgca actggaatgc cttgctttaa catgtccacc acggttttaa
     5581 aaactaagcg tttttgcgga tctgttgagt taatagaata ctcgattggt cgtcccttat
     5641 atcggccggc tttttgagcc aataatactc cttgccgctg acgttcacga attttctcac
     5701 gctcgttttg tgcaatataa cttagcagtg ataaaaacat atcgaacata gcggtatcca
     5761 ggagttcatt tccagtatta aaatctaaaa actgtgcatc taatattttt aacgctactt
     5821 ttttctgttt cataaaagca actgtatttt ttatatcttc ataatctctt cctaaacgat
     5881 ctagtgatgt aacgatgacg caatcacctt cacggatata atttagtaat ttttgaaact
     5941 caggacgctc agtatcttta ccactggctt tttcttggaa taatttatca atgttaagtc
     6001 cttccatcat tgccaattgt cgatccaaat tttgatcttg tgaagatact cgaatatagc
     6061 caatttttcc cataaaaaac tcctttttaa aagtgatata atttacatgt aaaatttact
     6121 tgtagtttta ctgctatcat agcattttta aaaataaatt ttatgtagac ttaaaattta
     6181 cttcatggag ggtttaaatg gatgaaatca aaagtaacaa aaaagcattt ggcatttatt
     6241 taggagaacc tagtacagaa caattaaacc aatttt
//
LOCUS       DAZKLD010000147         6267 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.148, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000147 DAZKLD010000000
VERSION     DAZKLD010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6267)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6267)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6267
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.148"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(41..229)
                     /gene="rpmB"
                     /locus_tag="ACG8KR_002529"
     CDS             complement(41..229)
                     /gene="rpmB"
                     /locus_tag="ACG8KR_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008536053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HFQ0201845.1"
                     /translation="MAKVCYFTGRKTKSGNNRSHAMNSTKRTVKPNLQKVRVMVDGKP
                     KKVWVSTRALKSGKVERV"
     gene            499..861
                     /locus_tag="ACG8KR_002530"
     CDS             499..861
                     /locus_tag="ACG8KR_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFQ0201846.1"
                     /translation="MAVKIQTSAGTIEITNDVIATVVGGAATDVFGVVGMASKNQIKD
                     NITEILRKENYSRGVVVRQEDNGIAVDVYTIISYGTKISEVSRNVQEKVKYNLEALLG
                     VTANSVNVFVQGVRVLPD"
     gene            913..2595
                     /locus_tag="ACG8KR_002531"
     CDS             913..2595
                     /locus_tag="ACG8KR_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HFQ0201847.1"
                     /translation="MSVTEISASQFQEMVQAGANRLQVNAEYVNSLNVFPVPDGDTGT
                     NMNLSMTSGAKAVVDSTSEKVGELAAMLSKGLLMGARGNSGVILSQLFRGFSKQIPDV
                     VSLNASDLAAAFTHGVETAYKAVMKPVEGTILTVAREAAKAGEKKAKSTDDVVEVMTA
                     VVKGAKRALAKTPDLLPVLKEVGVVDSGGQGLLFVYEGFLSALNGEFQADETYEPSPA
                     EMDDMVNAEHHRSVQGQMATEDIHFGYCTEIMVKIGEGPTVDSTFDYEEFRNYLDGIG
                     DSLLVVNDDEIIKVHVHTENPGEVLNYGQKFGSLVKIKVDNMRLQHETILEHDEEVNE
                     FAKKETPRIPYGIVAIAAGKGVQELFESLGANYVISGGQTMNPSTEDIVKAIQEVNAD
                     KVIVLPNNKNIFMAADQAAEVADIPVAVVPSRTISQGLTAMLSFNDQASLEENKETMT
                     EMLSSVVSGQVTHAIRDTSIDGVEIKEGDFLGMIDGKILISNPDITATALSTLEQMIN
                     EDTEIVTILAGDEGTVEQANEFAEKLTEKHPDLEIEIHQGDQPVYPYLFSAE"
     gene            2712..4748
                     /gene="recG"
                     /locus_tag="ACG8KR_002532"
     CDS             2712..4748
                     /gene="recG"
                     /locus_tag="ACG8KR_002532"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HFQ0201848.1"
                     /translation="MRSLDDPITMLAGVGEKRAASLASLGIQTIEDLLTYYPFRYEDI
                     QERNLNEIQDQEKVTLKGIVVSPPVMNRFGYKKSRLQFRMMQEHDVFNVSFFNQPYLK
                     EKVILSEEIAVYGKWDAKRKALNGMKILGSQTMDDFSPIYHVNKSIRQTTLVDLIKKG
                     FQEYGTLIEENLPNNLVEKYRLLDRASAVRSMHFPKDHEENHQAKRRVVFEEFFLFQM
                     RLQGLKKAEKAETNGLEILYDVQKLKEFTQKLPFELTNAQKRVTNEICRDLRSPQHMQ
                     RLLQGDVGSGKTVVAAIALYATVTAGFQGALMVPTEILAQQHMESLQQLFDPNEVTIA
                     LLTGSTKAKERRELLEKLEQGEINIVIGTHALIQDGVEFQNLGLVITDEQHRFGVNQR
                     KVLREKGWRPDVLFMTATPIPRTLAITAYGEMDVSIIDELPAGRIPIETRWVRTPQLD
                     SVLDWTKKELARGHQMYVICPLIEESEALDVKNAVEIYEKLRELFAPKYEVGLLHGKM
                     KNQEKEVIMEAFKDNQMQILVSTTVIEVGVNVPNATVMLIMDADRFGLAQLHQLRGRV
                     GRGSDASYCILVANPKNELGVERMKIMTETTNGFVLSEKDLELRGPGEVFGFRQSGLP
                     QFAIADLVTDGNVLEVAREEAAALWQIKDWQMLPEYQPLAQSLQIENQDNRYFD"
     gene            4797..5798
                     /gene="plsX"
                     /locus_tag="ACG8KR_002533"
     CDS             4797..5798
                     /gene="plsX"
                     /locus_tag="ACG8KR_002533"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HFQ0201849.1"
                     /translation="MKIAVDAMGGDHAPQAIVEGVALAQQDFPEVEFLLYGKEAEIKK
                     YLTNEKNITIIHTDEKINSDDEPVKAIRRKKTASMVLAAQAVKNGEADAVFSAGNTGA
                     LLAAGLFIVGRIKNIERPGLMSTLPVVGKEGAGFDMLDLGANAENKPEHLLQYGILGS
                     FYARKVRGIGRPRVALLNNGTEATKGSEVTKKAYELLQNETSLNFIGNVEARELLNGV
                     ADVVVTDGFTGNAVLKSIEGTAMNMMNLLKSAILNEGIKGKMGAMLLKDGLRSLKAEM
                     DYSKHGGAVLFGLKAPVIKTHGATGPDAVRYTIRQIHTMLETDVVGQLVEQFEKSEE"
     gene            5920..6162
                     /gene="acpP"
                     /locus_tag="ACG8KR_002534"
     CDS             5920..6162
                     /gene="acpP"
                     /locus_tag="ACG8KR_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFQ0201850.1"
                     /translation="MTREEVFNKVAKIISNHFEIDTDKVTDELNIKDDLKADSISIME
                     FVLELEDEFGTEISDEDAEQIETVGGAVDYISSHLK"
BASE COUNT         2080 a         1092 c         1430 g         1665 t
ORIGIN      
        1 aaaaaccgtt cactatctga acggtccaag tatcattcaa ttaaacgcgt tcaacttttc
       61 ctgatttcaa agcacgagtt gacacccaaa cttttttagg ttttccgtcg accataacgc
      121 gaactttttg taagttaggt ttaacagtac gttttgttga gttcatagcg tgtgaacggt
      181 tgtttccact ttttgtcttg cgacctgtaa agtaacaaac ttttgccatg gtaatttcct
      241 cctttgcctt gatttggagc aatagaataa ttcagctcat accagactaa catatcatat
      301 agaatggaaa tttgcaagta cattttcata gatacaattg acaattgaga agaataattg
      361 taaattaaac aaggttatac ctgaaatttt gcaaggtttt tgagtacttt gggtaactgc
      421 cttttatttc aagttgcgct atgataaaat atgatagaag aaaaatggga gttttttacc
      481 cgttaaggag gctcattcat ggctgtaaaa attcaaacgt cagctggaac cattgaaatt
      541 accaatgatg taattgcaac agttgttggt ggcgccgcga cagatgtgtt cggtgttgta
      601 ggaatggcca gcaaaaatca aattaaagat aatattactg agattttgcg taaagaaaat
      661 tactcaagag gagtagttgt ccgccaagaa gataatggta ttgctgtaga tgtttatacg
      721 attatcagct atggtacgaa aatctctgaa gtttcccgca acgtccaaga aaaagtgaaa
      781 tacaatctag aggcattatt aggcgtaact gccaattcag ttaatgtctt tgtacaaggt
      841 gttcgtgtac tgccagacta ggcaggagac tggctggtaa ctcagcagtc gatctgaagg
      901 aggattatct aggtgagtgt aacagaaata agtgcaagcc agttccagga aatggtccag
      961 gcaggtgcga atcgtctgca agtaaatgca gaatacgtca attcattaaa tgtattccct
     1021 gtgccagatg gcgatacggg aacaaatatg aatctatcta tgactagtgg agctaaggca
     1081 gtagtagatt caacctcaga aaaagtagga gaattagcag caatgctttc aaaaggattg
     1141 ctgatgggtg cgcgcggaaa ttccggcgtt attttgtctc aattgtttcg tggattttct
     1201 aagcaaattc cagatgttgt ctcattaaat gcttccgatc ttgctgcagc cttcactcat
     1261 ggagttgaaa ctgcgtataa agcagtaatg aaaccagtag aaggaacgat tttgacagta
     1321 gctagagaag cagcgaaagc tggagaaaag aaagcaaaaa gtactgatga tgtcgttgaa
     1381 gtaatgacag ctgttgtaaa aggggcaaag cgtgctttag ctaaaactcc agacttgctt
     1441 ccagtcctaa aagaagtcgg tgtcgtagac agtggagggc aaggactgct gtttgtttat
     1501 gagggctttt taagtgcatt gaatggagaa ttccaagcgg atgaaacata tgaaccttct
     1561 ccagcagaaa tggatgatat ggtcaatgca gaacatcatc gcagtgtcca aggtcaaatg
     1621 gcgacagaag atatccattt tggttactgt acagaaatca tggtaaaaat cggagaagga
     1681 cctacagtcg atagtacatt cgattacgaa gaattccgta actatttaga tggtatagga
     1741 gattctttac ttgtggtcaa tgatgacgag atcatcaaag tccacgtgca tacagaaaac
     1801 ccaggagaag tcttgaatta tggacaaaaa ttcggttcat tagtcaaaat caaagtcgac
     1861 aacatgcgct tgcagcatga gacgattttg gaacacgatg aagaagtgaa cgagtttgcg
     1921 aaaaaagaaa caccacgtat cccgtatggt atcgtagcga tcgctgctgg taaaggcgtc
     1981 caagagctgt ttgaaagttt aggcgctaat tatgttatca gtggtgggca aacaatgaat
     2041 ccaagtacgg aagacattgt taaagcgatt caagaagtaa acgcagataa agtgattgtc
     2101 ttgccaaaca ataagaacat cttcatggca gctgatcaag cagcagaagt agcagatatc
     2161 ccagtagcag ttgttccttc aagaacgatt tcccaaggat tgacagctat gctttcattc
     2221 aatgatcaag cttctttaga agaaaacaaa gaaacgatga cagaaatgtt gtctagcgtt
     2281 gtgagcggtc aagtcactca cgcgatcaga gatacctcaa ttgacggtgt agagatcaaa
     2341 gaaggagact ttttgggcat gatcgatgga aaaatcctga tctctaaccc agatatcaca
     2401 gcaacagcgt taagtacact agaacaaatg atcaatgagg acacagagat cgtaacgatc
     2461 ttggcaggtg atgaaggaac agttgaacaa gcaaatgaat ttgctgagaa actaacagaa
     2521 aaacatcctg atttggaaat cgagatccac caaggagatc agcctgtcta tccttatcta
     2581 ttttctgctg aataatttct gtgtcagaat atctttctaa cacagtattc attcataaaa
     2641 ggccttcggt tagtgatcag gtatgatcgc tgaccaagtc ctttttttgt taagggaggg
     2701 aggaaaaatt tatgcgttct ttagatgatc caatcactat gctggcagga gtcggagaaa
     2761 aaagagcagc tagtcttgcc agcttgggca tccaaacgat tgaagactta ctgacttatt
     2821 atcctttccg ctacgaagat attcaggaaa gaaatctaaa cgagatccaa gatcaagaaa
     2881 aggttacttt gaaaggaatc gtagtctcgc ctcctgtgat gaatcgattt ggttataaaa
     2941 aaagccgatt gcaatttcgt atgatgcaag aacatgatgt gttcaacgtt tcttttttta
     3001 atcaacctta tctaaaagaa aaagtcattt tatcggagga aatcgccgta tacggtaaat
     3061 gggatgcaaa gcgtaaagca ttgaacggga tgaagatttt aggttctcaa acaatggatg
     3121 acttttcgcc gatatatcac gtcaacaaat caattcgtca aacgacatta gttgatctaa
     3181 tcaaaaaagg gtttcaagaa tacggcacat tgattgaaga aaatctgcca aacaaccttg
     3241 tggagaaata tcgactgctt gaccgagctt ctgctgtaag aagtatgcat tttccaaaag
     3301 accacgaaga aaaccatcaa gccaaacgac gtgtcgtttt tgaagagttc tttttatttc
     3361 aaatgcgtct tcaaggattg aaaaaagcag aaaaagcaga aacgaatggt ttggagatcc
     3421 tttatgatgt ccaaaaattg aaagaattta ctcaaaagct accttttgaa ctgacaaatg
     3481 cacaaaaacg tgtaacgaat gaaatctgtc gggatcttcg cagtccgcag catatgcagc
     3541 gacttttgca gggagatgta ggtagcggaa aaacagtggt tgccgcgatt gcgttatacg
     3601 ctaccgttac agcaggtttc caaggtgcgt tgatggtgcc tacagaaatc ttggcacaac
     3661 aacatatgga aagcttgcaa cagctgtttg atcccaatga agtaaccatc gcattgctta
     3721 cggggtcaac gaaagcaaaa gaacgtcgtg agctccttga aaaactagag caaggggaaa
     3781 ttaacatcgt gatcggcaca catgcattga tccaagatgg agtcgaattt cagaatttag
     3841 gtctagttat tacggatgag cagcaccgtt ttggcgtgaa ccagcggaaa gtacttcgtg
     3901 aaaaaggctg gcgaccagat gtcttattta tgacagcgac tccgattcct agaacattag
     3961 ctattacagc atatggtgag atggatgtat ctatcattga tgaacttcca gctggacgta
     4021 tcccgatcga aacacgctgg gtaagaacac ctcaattgga cagtgtactg gattggacga
     4081 aaaaagaact ggcaagagga catcaaatgt atgtcatctg tccgctgatt gaagaatcag
     4141 aagcattaga tgtaaaaaat gcagtggaaa tctatgaaaa acttcgtgaa ctatttgcac
     4201 caaaatatga agttggcttg ttacatggta aaatgaagaa tcaggaaaaa gaagtaatca
     4261 tggaagcctt caaagacaac cagatgcaga tattagtttc gactactgta attgaagtag
     4321 gggtaaacgt gccgaacgcg accgtcatgc tgatcatgga tgcggatcga tttggattgg
     4381 cacagctgca tcaacttcgt ggacgtgttg gacgcggaag tgatgcttcc tactgtatct
     4441 tagttgctaa tcctaaaaat gaactaggtg tggaacggat gaaaatcatg acagagacca
     4501 ccaatggttt tgtactaagc gaaaaagatc tggaattacg tggtcctggt gaagtatttg
     4561 gcttccgtca atctggtctg cctcaatttg cgattgctga cttggtgacg gatggaaatg
     4621 ttttggaggt agccagagaa gaggctgcag ccctttggca gataaaggat tggcagatgc
     4681 ttcctgagta tcagccttta gcgcaaagcc tccaaataga aaatcaggat aatcgttatt
     4741 tcgattagca gactgtgcta tactttatac tgatgacgga aaaaggagtg gaaacaatga
     4801 aaattgccgt agatgcaatg ggcggagatc acgcacctca agcaattgta gaaggcgtgg
     4861 ctttagcgca gcaagatttt ccagaagtag aattcttact ttacggaaaa gaagcagaaa
     4921 tcaagaaata cttaacaaat gaaaaaaaca tcacgattat ccatacagac gaaaaaatca
     4981 atagtgatga tgagccagta aaagcaattc gacgaaaaaa gactgcatca atggtccttg
     5041 ctgcacaagc agtgaaaaac ggtgaagcag atgctgtatt ttcagcaggg aatacaggcg
     5101 cgctactagc agcagggttg ttcatcgtag gacgtatcaa aaatatcgaa cgccctggct
     5161 tgatgtctac cttgccagta gtagggaaag aaggtgccgg tttcgatatg ctggatcttg
     5221 gcgctaacgc agaaaacaaa ccagaacatt tgttgcaata cggtatttta ggttcttttt
     5281 atgcacgtaa agtccgaggt atcggacgtc ctcgtgtcgc actcttaaac aacggaacag
     5341 aagcaacaaa aggtagtgaa gtgacgaaaa aagcctatga actccttcag aatgaaacaa
     5401 gcttgaattt tattggaaac gtagaagctc gtgagctact caatggtgtg gctgatgtag
     5461 tggtcacaga tggatttaca ggcaacgctg ttttgaaatc tattgaagga acagcaatga
     5521 atatgatgaa tctattaaaa tctgctattc taaatgaagg gatcaaagga aaaatgggcg
     5581 ctatgctttt aaaagacggc ttacgttctt tgaaagcaga aatggattat tccaaacatg
     5641 gcggtgccgt tttgtttggc ttgaaagcac cagtcattaa aactcatgga gcaacaggcc
     5701 ccgatgctgt tcgctatacg atccgccaaa tccacacaat gcttgagaca gatgtagttg
     5761 gacaattagt cgaacaattt gaaaaaagcg aagaatagta tttggatatc tgaagcaatc
     5821 cgttccattg aaaaaaaggt ggacattcct gaaaaatgcc agtaaaatta gccatggtgt
     5881 aggtaattga caagtgtatg attcttggag gtgaaaaagt tgactcgcga agaagtattt
     5941 aacaaagtag cgaaaattat ttcaaatcat tttgagatag ataccgataa agtgacggat
     6001 gaattgaaca tcaaagatga tttaaaagct gattcaatta gtattatgga attcgtttta
     6061 gagcttgaag atgaattcgg tacggaaatt tcagatgaag acgctgaaca aattgaaaca
     6121 gtcggtggcg cagtagatta tatcagcagc catcttaaat aatttcaaat aaaagcaaag
     6181 aaggataaaa agctgcatac agctttttat ccttcttttt ttaatctttt tgacacgtaa
     6241 aagtcatttt tctgataatt ctggcga
//
LOCUS       DAZKLD010000148         6175 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.149, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000148 DAZKLD010000000
VERSION     DAZKLD010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6175)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6175)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6175
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.149"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            233..482
                     /locus_tag="ACG8KR_002535"
                     /pseudo
     CDS             233..482
                     /locus_tag="ACG8KR_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001206386.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(726..1556)
                     /locus_tag="ACG8KR_002536"
     CDS             complement(726..1556)
                     /locus_tag="ACG8KR_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HFQ0201851.1"
                     /translation="MFRHQKELQFEAKPDRPNPLIAKYLQELIGGQYGEMSVAMQYLF
                     QGWSCRGEEKYKDMLMDIATEEIGHVEMLATMVARLLEGAPVEDQEAAMQKDPTIGAI
                     ISGMNPQHAIVAGLGPRPADSVGNPWSGAYIIASGNLLADFRANLNAESQGRLQVARI
                     YEMIDDRGVKDMLSVLLARDSYHQNLWECAVKELEEKEGSILVPSTFPRDKERKDIAY
                     DFYNFSEGDQSSEGSWASGKALDGEGEYQWLKEPKIEGAAPKLEPVTPKMYGTPPFKK
                     "
     gene            1764..3098
                     /locus_tag="ACG8KR_002537"
     CDS             1764..3098
                     /locus_tag="ACG8KR_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFQ0201852.1"
                     /translation="MKKLLFTGLLASSALFLLGGCGSSNASDASSSAANSSEKTSDGR
                     KNIDFWSFWGSGARKEAIEEIIEDFNQSQNEIHVNYKYQPWGDIWTKSLSAITAGNAP
                     DVIVQDINSVAQRAEAQQATNLSKYVEKDTSKDFYPQLWDTVEYEGDPYAIPFNTDTQ
                     VIFYNKKLFKEAGISEQDLPTTWDELEETARKLDKKEGKEFTRIGFYPLWNLGADVWA
                     LNADNGTSWFDKDEKVKIDTKNKVEALEWIQDWQDYYGKNTINKLEAEFGSGVSDPFI
                     SGLVAMRAQNINYYSSLMENAPDDFDFGVMQLPEKEEGSGHWSWGGGFVLEVPYGAKD
                     PEASYEFIKYLSSPEVQEKFGEKSFDIMASKTANENLVNNDNLTDKGKMIYQMADENF
                     KNTVITPVPLDAPDYTTLVNEQIDQILLGTKSPKEGLADAQKAVENLVKQNQ"
     gene            3139..4050
                     /locus_tag="ACG8KR_002538"
     CDS             3139..4050
                     /locus_tag="ACG8KR_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0201853.1"
                     /translation="MKRLSIRKKKEARWAYLFISPFIIGFLVFMLGPMLFSVIGSFTD
                     YNLTSKMNFIGLANFKRMFVHDDLFWKSLYNTVYFVIFNVPLTTIFSVFLATVLNQKI
                     KGISVFRTAFYLPAVLSGVAVYILWMQLLSPSAGLINTFLGWFHIPGPAWLFDPQWTK
                     PALILMKIWSSGGAMLLYLATLQNVPRSLYESAEIDGAGVWGKFKSITLPLITPIIFY
                     DVITSTIGSFQIFQEAYVMTKNGTGGPANSLLFYNLHMWNKAFVAFDMGYAMAMSMIL
                     FLIVLVLTFVNLKLAPKWVYYSGGDGK"
     gene            4047..4901
                     /locus_tag="ACG8KR_002539"
     CDS             4047..4901
                     /locus_tag="ACG8KR_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0201854.1"
                     /translation="MMDYFKKTSVNRQTKKILSYVLMTVIGIILIIPLLWMVFTSLKP
                     MEEIVRYPPTFFPEKIVWENYLDTIAAFPFWRYARNTLFITVLVVIGNVLSNSFIAYG
                     FAKLDFPGKKLMFALVLSTMMIPGFVTMIPQYVLFSKIGWVGTYLPLIVPSFFGNAFN
                     IFLMRQFYLSINNELIEAAEIDGANHLYIWSHLMLPLTKPALITIAINSFNAAWNDFL
                     GPLLYIQDQEKYTLQIGLQVFQNQATTQWNYLMAGATLVLIPTILLFFFAQRYFIEGM
                     DLTGGSKG"
     gene            4898..5515
                     /locus_tag="ACG8KR_002540"
     CDS             4898..5515
                     /locus_tag="ACG8KR_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HFQ0201855.1"
                     /translation="MMNRSIGKKANDYFLEIIEWIPRLIHLQLLWFLCVLPVFTLGTA
                     SRTVLYMIYHYKKNEEKKLGSLFWKKFRENFSLYGKQDSLASVYFLLLLIDYQIFRYW
                     GGGIGYTLMYTTLFLLLLSALLFSYKILLQLEQKEASWITAFFLFFNDFKSALFYLAG
                     TLILLVAFWFAGPIYFALLGFSFLFYFQVLLFIHRTQEKVLKKEE"
     gene            5517..6035
                     /locus_tag="ACG8KR_002541"
     CDS             5517..6035
                     /locus_tag="ACG8KR_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HFQ0201856.1"
                     /translation="MLRLIAKIDDSIINKFAKQRKTDSRKINFLKFISNISNGGFIWL
                     LLAAVLVLIPDTREYGLAILIGSLIYFFVGLLFIKKTTRRKRPYEKNRRLVLYTSKPK
                     TSSFPSGHTALAFLSVAALFELSPVAGILAFLFACLIAYSRIFLLLHYPTDILGGAIL
                     GSVIGILCAAVI"
BASE COUNT         1844 a         1100 c         1235 g         1996 t
ORIGIN      
        1 cttcttgaag cattttccat ttataaaaaa tcaaaaaaat aaaatagatt gccgtaagag
       61 atctttttca tttcattttt ttctgaaaac ccgttgtttc tgtcagtgaa gcagcgggtt
      121 tttagattta ttgtctttag acctaattga gcgcgccgag cgattgaaat acaaaaccac
      181 ttgctatgcg ggtggagcaa taaagggaaa tagtttagcc cgtacaaagt agatgtgtaa
      241 ataccacatt gtattcacac caaagtatag aagaaaaata atttataatc aatatcgagc
      301 caatattcag gagatcatca agagattgtg caaatctaaa atataaattt gggtatcgac
      361 acttttgggt ggaaggatac tatgtgacaa aagtaggaca aaatgaagcg accatcaaaa
      421 agtacattca agaacaagaa aaacatgata gtgcgttaga taagctgagt gtacgagagt
      481 aagacgatcc ttttaagggc taaggagtag tacataaacc ttttaagagg tgggcaaaac
      541 tggcaaaggc aatatggctt gaacataaaa tatgaaagcc agcgtcttta ggcgctggcc
      601 ggtgatttga gcttatagct ctagagaaaa ccaccctttt gaagggtggt tatgattaat
      661 gactttagtc agttttgctc acaaagcgtg tggaacaaaa aacgggtgtt tctacgggta
      721 gatatttatt tcttgaatgg aggtgtccca tacatttttg gtgttactgg ttctaatttt
      781 ggtgcagcgc cttcgatctt aggttctttc aaccattgat attcgccttc gccatccagt
      841 gctttgccac tagcccaaga accttcacta ctttggtctc cctctgagaa gttatagaag
      901 tcataagcaa tgtcttttcg ttctttgtca cgcgggaaag tacttgggac taagattgaa
      961 ccttcttttt cttctaattc tttcactgca cattcccata gattttgatg atagctatct
     1021 cttgcaagga gaacagacag catatctttt acgccacgat catcgatcat ttcgtaaata
     1081 cgtgctactt gaagtcttcc ttgtgattcg gcatttaaat tagctctaaa gtctgctaac
     1141 aagttgccac tcgcaatgat ataggcaccg ctccatggat tgcccacgct gtcagcagga
     1201 cgaggtccta agccagctac gatcgcgtgc tgtggattca ttccagagat aatggcaccg
     1261 attgttggat ctttttgcat cgctgcttct tgatcttcaa caggcgcacc ctctaataaa
     1321 cgagcaacca ttgtcgccaa catttccacg tggccgattt cttctgttgc aatgtccatc
     1381 agcatatctt tatatttttc ttctcctcgg cagctccagc cttggaacag gtactgcata
     1441 gctacactca tctcgccata ttggccgccg atcagctcct gcagatattt cgcgattaat
     1501 ggattcggac gatctggttt tgcttcaaac tgcaattctt tctggtgtct aaacatcaaa
     1561 attcctccta atctattttt aatacattct cattctgcta cgaaagatta aaaaattcaa
     1621 agaaagcgct cgcgaattat ataaagtatt ggtatcatta ataatttttt tagaatcata
     1681 ccttataaat tgttattttc agaacaaaat gatatcgtta tattaaaacc ggcatcggtt
     1741 acattaaaat ttggaggtaa aatatgaaaa aattattgtt tacagggttg ctggcaagtt
     1801 cagcattgtt tttattagga ggatgcggat caagtaatgc aagcgatgcc agttcaagtg
     1861 cagcaaattc ttctgaaaaa acaagtgatg gaagaaaaaa tattgatttc tggtcctttt
     1921 ggggatccgg tgcacgaaaa gaagctatcg aagaaatcat tgaggatttc aatcaatcac
     1981 aaaacgagat ccatgtaaat tataagtatc agccatgggg tgacatctgg actaaatctt
     2041 tatctgcaat aacagctgga aatgcacccg atgttattgt acaggacata aattccgttg
     2101 cccaaagagc agaagcacag caagcaacta atctctccaa atatgtagaa aaagacactt
     2161 ctaaggattt ttatccgcag ctttgggata cagtagaata tgaaggtgat ccttatgcga
     2221 tcccattcaa tacagataca caagtcattt tttataataa aaagttattt aaagaagctg
     2281 gaatcagtga acaagatcta ccgacaactt gggatgaatt agaagaaaca gcacgtaaac
     2341 tagataaaaa agaaggaaaa gaatttacac gcatcggttt ttatccatta tggaatttag
     2401 gggctgatgt atgggcgcta aacgcagata acgggactag ttggtttgac aaagatgaaa
     2461 aagtcaagat tgatactaaa aataaagtgg aagccttgga gtggatccaa gactggcaag
     2521 attattatgg gaaaaatacg atcaataaat tagaagccga atttgggtct ggtgtatctg
     2581 atccatttat ttccggatta gtagccatgc gtgcccaaaa tatcaattat tactctagtt
     2641 tgatggaaaa tgcaccggat gattttgatt ttggcgtgat gcagctccca gaaaaagaag
     2701 aaggttcagg ccattggtcg tggggtggcg gattcgtctt ggaagtacca tacggagcaa
     2761 aagatcctga ggcctcttat gaattcatca aatatctttc ttctccagaa gtacaagaaa
     2821 aattcggcga aaaaagtttt gatatcatgg cgagcaagac agccaatgag aatttagtga
     2881 ataatgacaa cctaacggat aaaggaaaaa tgatctatca aatggcggac gaaaacttta
     2941 aaaatacggt gatcacacca gttcctttag atgctccgga ttacacgaca ttagtgaatg
     3001 aacagattga ccagattctt ttaggaacaa aatcaccaaa agaaggattg gcagatgcac
     3061 aaaaagcagt agaaaactta gtcaagcaaa atcaataatc catttttgac tgtctaggta
     3121 cagaggaggc aagaaaattt gaagcgttta tctatccgaa agaaaaaaga agcaagatgg
     3181 gcctatttat ttatctcgcc ttttattatc gggttcttgg tatttatgct agggcctatg
     3241 cttttttcag ttattggtag tttcaccgat tataatttga catccaagat gaatttcatt
     3301 ggtctagcaa atttcaaaag aatgttcgtt cacgatgatt tattttggaa atcgttatac
     3361 aacaccgttt atttcgtcat cttcaatgta ccgctgacga cgattttcag tgtgttttta
     3421 gccacagtac tcaatcaaaa aatcaaagga atcagtgtat tccgtacagc tttctattta
     3481 ccggcagtct tatcaggagt agcagtctac atcttatgga tgcagctgct ttctccatct
     3541 gcaggattga tcaacacatt tctgggctgg ttccatatcc ctggaccagc ttggctgttt
     3601 gatccacaat ggacgaaacc tgcgttgatt ttgatgaaaa tatggagttc tggaggagca
     3661 atgctacttt acttagctac attacaaaat gttcctagat ctttatatga atcagcagaa
     3721 atcgatggag caggcgtttg gggaaagttc aaaagtatta ctttgccatt aatcactcct
     3781 attatttttt acgatgtgat cacctctacg atcggttctt tccaaatttt tcaagaagct
     3841 tatgtcatga cgaaaaatgg aacaggcgga cctgcaaatt ctttgctttt ttataatctg
     3901 catatgtgga acaaggcgtt cgtagctttt gatatgggtt atgcgatggc tatgtcaatg
     3961 atcctgtttc tgatcgtgtt ggttctgacg tttgtcaatt tgaagcttgc acctaagtgg
     4021 gtctattatt ctggaggtga cggcaaatga tggattattt caaaaagacc agtgtgaatc
     4081 ggcaaacaaa aaaaatacta agttatgtcc tcatgacagt aataggaatc attttgatca
     4141 ttccattgct ttggatggtg tttacttcat tgaaaccaat ggaagaaatc gttcgttatc
     4201 ctccgacctt tttcccagaa aaaatcgtat gggaaaacta tctggatacg attgccgcct
     4261 ttcctttttg gcgctatgca agaaatacat tattcatcac tgtgctagta gtgatcggta
     4321 atgtcctttc aaattctttt attgcttatg gatttgcgaa gcttgatttt cctgggaaaa
     4381 agctgatgtt tgctttggtt ctttctacga tgatgatccc ggggtttgta acgatgatcc
     4441 ctcaatatgt attattctcc aagatcggct gggtaggtac ttatttgccc ctgatcgttc
     4501 catcgttctt cggcaatgcg ttcaatatat ttctgatgcg tcaattttac ttatcgatca
     4561 ataatgaatt gatcgaagct gctgaaatcg acggagcgaa tcatttgtat atttggagtc
     4621 atttgatgct tccattgacg aaacctgcct tgattaccat tgcgatcaat tccttcaacg
     4681 cagcttggaa tgatttctta ggtcctctgt tatatatcca agatcaagaa aaatacacgt
     4741 tacagattgg cttgcaagtt tttcagaacc aagcaacgac acaatggaat tatctgatgg
     4801 caggagcaac acttgtcttg atcccgacga ttttgctgtt cttttttgca caacgttatt
     4861 tcattgaagg gatggatttg acaggaggtt caaaaggatg atgaatcgat cgattggtaa
     4921 aaaagccaat gattatttct tagaaatcat tgaatggatt ccacgtctta tccatttaca
     4981 gctactatgg ttcctgtgcg tcttgcctgt tttcacatta ggaactgcaa gtcgaacagt
     5041 tctgtatatg atttatcatt acaaaaaaaa tgaagagaaa aagctcggtt cactattctg
     5101 gaaaaagttt cgtgagaact tctctcttta tggaaagcaa gatagtttag caagtgttta
     5161 ttttcttctt ctattgatcg attatcagat tttccgctat tggggtgggg gaattggcta
     5221 tacattgatg tatactacgt tgtttttgct tctattaagc gcgcttctct ttagttacaa
     5281 aatcttgctt caactggaac aaaaagaagc ttcttggatt actgcatttt tcttgttttt
     5341 taatgatttc aaaagcgcgc ttttctattt ggcaggcacg ttgatccttc ttgtcgcatt
     5401 ctggtttgca ggaccaatct attttgcttt attaggtttc agtttcttgt tttattttca
     5461 agtcctgctt tttatccatc gtacgcaaga aaaggttctt aaaaaggagg aataacatgt
     5521 tgcgtttgat tgctaaaatc gatgatagta ttatcaacaa gttcgccaag caacggaaaa
     5581 cggatagtcg aaaaatcaat tttttgaaat ttatcagtaa tatctctaat ggtggtttta
     5641 tttggttgct ccttgctgct gttttagtct tgatacctga tactagagaa tatggtctag
     5701 ccattcttat cggcagtttg atctattttt ttgttggttt gctttttatc aaaaaaacga
     5761 caagacggaa aagaccttat gaaaaaaatc gtcggcttgt cctttacaca agtaaaccga
     5821 agacttcttc ttttccttca ggacatacag cgctagcctt tttatcagta gctgcgttgt
     5881 tcgaattaag tccagttgca ggaatattag cctttttatt tgcttgtttg atcgcttatt
     5941 cgaggatttt tttactttta cattatccta ctgacatttt aggaggagct attttaggaa
     6001 gtgttattgg catcctatgc gctgccgtta tctgaattaa ttgtacgaat aaagcatgag
     6061 tgcgtaaatg cgcatttgtg ctttattttt ttgtttcgag gaaagcgtag ttgtttattc
     6121 atttgtctta ataacttaag ttttgttaaa tgagaacgtt ataaaatttt ataat
//
LOCUS       DAZKLD010000149         6077 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.150, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000149 DAZKLD010000000
VERSION     DAZKLD010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6077)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6077)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6077
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.150"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            50..1249
                     /locus_tag="ACG8KR_002542"
     CDS             50..1249
                     /locus_tag="ACG8KR_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HFQ0201857.1"
                     /translation="MNLTRDLIFLVLYLIGVFSIVYWRKQKKDPSLFFYGSTWTFTVI
                     LTYFVVLEIFYSTFYFLIPLLFFSIFAFSYFTEKRRLLNGLLFNVLLISFGIYLFVLL
                     YETQNIFLGGLIALITIPLLLVLLFGIYGLIVFLFWNGVTVLRRESHSLANLLTLILA
                     IFLTLFLVFDFFLLKYLPQWINALFFCVPLILIYLFIVFYNFLTVSFLYQFNRPRYNQ
                     DFIVVLGAGLINGETVSPLLAKRINKAIAFYRAQSRATLNPPILLMSGGQGADEKVPE
                     AIAMKQYAMEQGIPERDILVETNSTTTLENMLYSKEIMDQQMKGPYRAIFSSNNYHIF
                     RAGLYARQAKLKANGIGAKTAFYYLPNAFLREYLAILMMNKKRHMIICGLLFAGSAFL
                     SLITLLF"
     gene            complement(1276..2544)
                     /locus_tag="ACG8KR_002543"
     CDS             complement(1276..2544)
                     /locus_tag="ACG8KR_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HFQ0201858.1"
                     /translation="MEKQLPSPVRILVYMLKWLVITAILGIFMGSLSAFFLNSLTVVT
                     DIRLSHPWLLCLLPVSGALFAYLYAYHGGLSSRGNNLVIDQGNGGEEKIPLRLIPLTL
                     FGTITTHLFGGSVGREGTAVQMGGALADNTARLFRLDKSEREILIISGISAGFSSVFG
                     TPLAGTLFGLEVLAIGKVRTEAIFPSFFAALFANFMTESYGVSHLHYQMGVIPEWSML
                     LFFKVFVAGIAFGLIGWVFSRSIVFLKARYAQWIPHPVCRNTIGGAVVVAAALILQTQ
                     RYLGLSLPLLQESFNGQAHVYDFIGKLFFTVLSLGAGYQGGEVTPLFEIGATLGAALA
                     PLLHVSVPFLAGLGFIGVFSGATNTPIACFVMGIELFGSQAAVYFFMICLISFMCSGN
                     SGIYSAQQVAVKKGILFLPIVKRMKEKNKV"
     regulatory      complement(2599..2665)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01734"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Fluoride riboswitches increase expression of
                     downstream genes that help mitigate the toxic effects of
                     very high levels of fluoride; They are found in a wide
                     variety of bacteria and archaea; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="fluoride"
                     /db_xref="RFAM:RF01734"
     gene            complement(2707..3353)
                     /locus_tag="ACG8KR_002544"
                     /pseudo
     CDS             complement(2707..3353)
                     /locus_tag="ACG8KR_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728800.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FusB/FusC family EF-G-binding protein"
     gene            complement(3761..4264)
                     /locus_tag="ACG8KR_002545"
     CDS             complement(3761..4264)
                     /locus_tag="ACG8KR_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFQ0201859.1"
                     /translation="MTEKWREDEEYMSYVQDLLETEAVKKLANYTQHVHSTRLEHSIS
                     VSYYSYLLAKKWGGNAKATARAGLLHDLFYYDWRTTKFDEGTHAYIHPRIAVKNAEKI
                     TDLSDLERDIILKHMWGATIAPPKYKEGYIVTFVDKYCAVKEAAQPMSASMRKRWQRY
                     FGKESSI"
     gene            complement(4387..5151)
                     /locus_tag="ACG8KR_002546"
     CDS             complement(4387..5151)
                     /locus_tag="ACG8KR_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HFQ0201860.1"
                     /translation="MKEIQSVKNPRIKELKKLHKKKYREEKAEYLLEGFHLIEEAASS
                     DAEIKELFVTQRGMKEWGDWLAVNKEISTFLVSDEVMTAMSDLPTPQGILAVMTLTSE
                     DTPDFSGGWLLLDNVQDPGNVGTMIRTADAFGMAGVLLGKGTADIYSTKVLRSMQGSN
                     YHLPVLQKDLAEVIPVMKGKGFTVYGTELNEEAHALDKVEKTEDFAIIMGNEGQGVSQ
                     EILSSTDQNVYIPMKGNAESLNVGVAAGIVLYHFSL"
     gene            5258..5533
                     /locus_tag="ACG8KR_002547"
     CDS             5258..5533
                     /locus_tag="ACG8KR_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HFQ0201861.1"
                     /translation="MRKVKMNVQGRVQGVGFRYMTKMVADQLGVTGTVKNEDDGSVTI
                     EAIGNDDIIQKFIEEVKKSPSPSGRVQYVDVQEDPMIEERKKFDVVG"
BASE COUNT         1778 a         1271 c         1085 g         1943 t
ORIGIN      
        1 ttttttcatc tattttcgtt atactgtata agaaaaagga gttgaattta tgaatctaac
       61 cagagatctg atctttttag ttctctatct tattggggta ttctctatcg tttattggcg
      121 aaaacagaag aaggatcctt ccttgttctt ttacggcagt acgtggacat ttacggtaat
      181 cttaacttat tttgtcgtgt tggaaatttt ttattcgacc ttttattttt tgattccatt
      241 gttgttcttt agtatctttg ctttttctta tttcacagaa aaacgaaggc tactgaatgg
      301 tcttttattc aatgttttac tcatcagttt cggcatctac ttgtttgtct tgctgtatga
      361 aacgcaaaat atttttcttg gcggactgat cgccttgatc acgattccat tgctacttgt
      421 ccttttattt ggtatttatg gattgattgt ttttcttttt tggaacgggg tcacagtttt
      481 acgtagagaa tcgcattcat tagctaatct tttaacgttg atactagcca tttttcttac
      541 actgtttctt gtttttgact ttttcttgtt aaaatatttg ccgcaatgga taaatgcttt
      601 gtttttctgc gttcccctga tcttgatcta tctatttatc gtattctaca actttttgac
      661 tgtctcattc ttgtatcagt tcaaccggcc tcgatacaat caagacttca tcgttgtgtt
      721 aggcgctgga ttgatcaatg gagaaacagt ttctcctctt ctagccaagc gaatcaataa
      781 ggcaattgct ttttatcggg ctcaaagccg tgctacatta aatccgccca tcttattgat
      841 gtctggcggg caaggagcag acgaaaaagt accagaggcc attgcgatga aacaatatgc
      901 catggaacaa ggaatccctg aaagagacat tcttgtcgaa accaattcga caaccacttt
      961 ggaaaacatg ctctattcaa aggaaatcat ggatcagcaa atgaaagggc cataccgcgc
     1021 gatctttagt tccaacaatt accacatttt tagagccggt ctttatgcgc ggcaagccaa
     1081 attgaaagca aatggtattg gtgcaaaaac agctttctac tacttgccta atgcgttttt
     1141 aagagaatac ctcgccatct tgatgatgaa caaaaaaaga catatgatca tttgcggatt
     1201 gcttttcgca ggctccgcat tcttatcgct gattacttta cttttctaaa aaaacaaggt
     1261 gtgacaaaac atatgtcaca ccttgttttt ttctttcatt ctcttaacta ttggcaaaaa
     1321 gagtatccct tttttgaccg ctacttgctg agcagaataa ataccgctgt tgcctgaaca
     1381 cataaagctg atcaagcaga tcataaagaa gtatactgcc gcttgtgaac cgaacaattc
     1441 gatccccatc acaaagcagg caatcggtgt atttgtcgca ccagaaaata ctccgataaa
     1501 gcccaatcct gcaagaaaag gaacagaaac atggagcaaa ggcgctagtg ctgcaccaag
     1561 cgttgcacca atttcaaaca atggtgtcac ttctccccct tgatatcccg cgcctaaaga
     1621 caaaactgtg aagaataact tcccaataaa atcatagaca tgtgcctgtc cattaaatga
     1681 ttcttgaagt aacggaagac tcaatccgag ataacgttgt gtttgcaaaa tcaatgctgc
     1741 tgccacaact acagctccac ctatcgtatt tcgacaaaca ggatgaggaa tccattgtgc
     1801 atatcgcgct tttaaaaaga cgatcgaacg gctgaatacc caaccaatca aaccaaaagc
     1861 aatcccagca acaaaaactt tgaagaacag caacatactc cattcaggaa tcacacccat
     1921 ttggtaatgt agatgagaaa caccatagct ttctgtcatg aaatttgcaa acagtgcagc
     1981 aaagaagcta gggaagatcg cttctgttcg gactttcccg attgccaaca cttctaatcc
     2041 aaataatgtt ccagctaaag gtgtgccaaa tacagaacta aaacccgcac tgattccact
     2101 gataatcaag atttcccgtt ctgatttatc caatcggaac aaacgtgccg tattatctgc
     2161 taacgctccc cccatttgga ctgctgttcc ttcacggcca acagatcccc caaataaatg
     2221 ggtagttatg gttccaaaca gtgtaagtgg aataagccgc aacgggattt tttcttctcc
     2281 accatttcct tgatcgatca ctaagttgtt tccacgacta gaaagtccgc cgtgatacgc
     2341 atagagataa gcaaaaagcg caccactaac tggtagtaga caaaggagcc aaggatgtga
     2401 tagtcggata tctgtcacaa ctgtcaggct atttaagaaa aaagcagaca agctccccat
     2461 gaatataccg agaatcgctg tgatcaccag ccacttcagc atatacacta aaatgcgtac
     2521 tggactcggt aattgttttt ccataaaaaa ttcctcctca ataaaaaaac gcctactaaa
     2581 gaaaggggct gtccccagac tttagtaggc gtcattagct atctttcgtt agcatttaaa
     2641 ggcgaacacc atcacctatt cacttccatt aaaagatagc atatttttct gtaaaaaaca
     2701 ataaaattat cgttctttct ttacacgttc agcaaattca tagaaaggag ccaatttcgt
     2761 taactgttga ttacattggt cactatcatg acaaatataa ttcccctttt tcgtatacgt
     2821 gccatcagaa cctgctttcg ttgtagaaag aaaaagcgaa acattcgatg ttttctgaca
     2881 gattgcacag attcccttat ttatcgttgg tgataaagta ccgctaatcc cttttaattt
     2941 ttcatcttcg taatagacca tgaatttttt ctgcgtacct ggatcattcc agccgatgta
     3001 cgtatgttct tgcaagtcga tttcattcca agctggttgt ttgagttttt tcacttttcg
     3061 gaataatttc tcgatttgtg gtttgctagg ctgcataaac ggctgaacga tttgcttcag
     3121 ctcctccaat gctctttctg cttgcgcttt tgtcagtttt acgtctaaaa ctgtcgttaa
     3181 aaatgtatcg attctggcag ttcgtgatct aatatgtctc gaatcttttc agctgctagt
     3241 gcctgaactg tttgtaccgt ttttggatca ttgacagatg agtacgcatt taataactgt
     3301 tctacttctc ttattataaa aaaatattgg tatggtttta ttgtttgttc catgatttta
     3361 aagtcctttc ttctaaccaa agtgcatact ttgtagaaaa aagtgatctt ctgcaaagta
     3421 tgtgttacct gaagatcctg gactctttta aataagatac gttttgcatc ttatcatcac
     3481 cccctttaat gcttattatt ctatgtacat gattcattcg ataaataaaa gtaaaccgta
     3541 tcttgaacag atagtcaact gcaaacagaa aaaaggactg tgacaaaagt cgtgtcaaag
     3601 tcccttgttt cgaatgaatg gtgttcaaaa gcttgcatct gcggtaataa gccgaaactt
     3661 cacaaaaatt tgagaagtaa ttttcgtaaa ttccttctta tttcttgaag ctgaccactt
     3721 ctgtcacaac ctcaatatgg tctattattg gttttacctg ttaaatcgag gattctttcc
     3781 caaagtaacg ttgccatctc ttacgcatac ttgcagacat gggctgagcc gcttctttga
     3841 ctgcgcagta tttgtccacg aatgtcacaa tatatccttc tttatactta ggcggtgcga
     3901 tcgttgcacc ccacatgtgc ttcaaaatga tatctcgctc aagatccgac aaatcggtga
     3961 ttttttctgc attctttaca gcaattcttg gatggatata cgcatgtgtc ccctcgtcaa
     4021 atttcgtcgt acgccaatca tagtagaaca gatcatgcaa aagtccagca cgcgctgttg
     4081 ctttagcatt gccaccccat ttttttgcga gcagataact atagtaagaa acactgatgg
     4141 aatgctccaa acgagtggaa tgaacatgct gtgtgtaatt cgctaatttt ttcactgctt
     4201 ctgtttctaa aagatcttgt acataagaca tatattcttc gtcttcacgc catttttctg
     4261 tcataattat cgatttgcca cctttttgaa tttcattttc ttctgaaatt gatctaagta
     4321 taccaccagt gttcggatta ttctagtcgc tctagataaa atagctaaag cttaatagtt
     4381 ccttaatcat aaagaaaaat gataaagaac gatccctgct gcgactccga cattcaatga
     4441 ttccgcattt cctttcattg ggatatatac gttctgatca gtcgaagaca agatttcctg
     4501 acttactccc tgaccttcgt ttcccatgat tatagcgaaa tcctctgttt tttctacttt
     4561 gtctaatgca tgtgcttctt catttaattc tgtaccgtac accgtaaacc ctttgccttt
     4621 cattacaggg atcacttctg ccaaatcttt ttgtaacaca ggcaaatgat aattgcttcc
     4681 ctgcatgctt cgcagcacct ttgtactata aatatctgcc gttcctttac ctaataaaac
     4741 accagccata ccaaacgcat ctgccgtacg gatcatcgtt cccacatttc caggatcctg
     4801 tacattatct agtaaaagcc agcctcctga aaaatctggc gtatcttcag atgttaacgt
     4861 catcactgct aagatccctt gcggtgtagg tagatctgac atagcggtca tgacctcatc
     4921 tgaaactaga aaagtagaaa tctctttgtt tacagccagc cagtctcccc attctttcat
     4981 acctctctgt gtcacaaaaa gttcttttat ttctgcatca gacgacgcag cttcttcgat
     5041 cagatggaac ccttccaaaa gatattctgc cttttcttct ctgtatttct ttttatgtag
     5101 tttttttaat tctttgattc ttggattttt tactgattgg atctctttca cattcctcac
     5161 ctcaactgat tatatcatgg aataggtgct tgagcttgtt tcatcctaaa aaaatcggta
     5221 tcattgattt ataaaacagg aaagaaaggt gacgaacatg cgcaaagtta aaatgaatgt
     5281 ccaaggacgt gtccagggcg tgggatttcg ctatatgacc aagatggttg ctgatcagtt
     5341 aggcgtgacc ggaacggtaa aaaacgaaga cgacggctca gtcacaatcg aagcaatcgg
     5401 aaatgacgac atcatccaaa aatttatcga agaagtcaag aagtctccga gcccaagtgg
     5461 acgagtgcag tatgtggatg tccaagaaga tccgatgatt gaggaacgaa agaaatttga
     5521 tgtggtggga taaggttgtt tttgaagcgc tttgcctcga gtattaagga gaaacatttt
     5581 ccaaatagct tctcatattt tgaaatgtaa tgacttaata ccgagaggca gctgataaaa
     5641 caccgtcaat aaggttgtga aaaagctggt ctgcatcaag cattaaggac tggttgtgaa
     5701 aaatagctct ccatattttt caacaggctg gacttaatgc cgtagatgca agcttttgaa
     5761 catcgtcgat aaggttgcga aaaagctgat ctgcatcaag cattaaggac tggttgtgaa
     5821 aaagtcgctt agttttgagt attaaggaaa attttgtgaa aatggctttc cacatttttg
     5881 cataaatttg acttaatacc aagaaactga cttttgaaca tcgtcgataa ggttgttttt
     5941 gaggcacttt gcctcaagta ttaaagacag gtagtaaaaa agctgatcta taacttgcaa
     6001 taaatttgct taacccatat tttccagaca gaacaaaaaa gtttttagtt ttttaaataa
     6061 gaaagaaaca cttggaa
//
LOCUS       DAZKLD010000150         5989 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.151, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000150 DAZKLD010000000
VERSION     DAZKLD010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5989)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5989)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5989
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.151"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(76..1170)
                     /locus_tag="ACG8KR_002548"
     CDS             complement(76..1170)
                     /locus_tag="ACG8KR_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HFQ0201862.1"
                     /translation="MGKLGISIYPERSTFEKDKAYLDLAHKYGYKRVFTSLLQINDDK
                     EKVLSEFKEVVDYANSLGMEVMVDINPALFEQLEISYDDLSFFHKMGAYGVRLDIGFT
                     GAEEAKMTRNPFGIKIEINMSSGTNYVDNIMSYSPNTDNLLGSHNFYPHRYSGLGYEH
                     FVFCSEKFRKYNLNTMAFVNSQSAEFGPWPTQDGLCTLEDHRDLEIATQVKHLILTGL
                     IDDISIGNAYASEEELKEMAEAFNADYPTLKVDTEEGITENERICLFDNLHSYRGDRS
                     EYILRSTMTRVYYKDKDFPPHNTRDMHHGDVLIDNEGYGQYKGETQIALKDMKNDGRV
                     NVVGRISDDELFLLDFLKPWSSFKLIENKK"
     gene            1359..2120
                     /locus_tag="ACG8KR_002549"
     CDS             1359..2120
                     /locus_tag="ACG8KR_002549"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFQ0201863.1"
                     /translation="MKKNKVILSISLAAALLFAAGCSANNTTSSSSSTESSKSTSTAE
                     STTASSVDLNALELPQLTKDVAENEDLVEMVTNKGTIKIKLFPEHAPKAVENFMTHAK
                     DGYYNGLTFHRVIKDFMIQGGDPKGDGTGGESIWGEGFETEINNHLYNIRGALSMARS
                     QDPNSNGSQFFIVQNTDDMHDGLLKDDYPQAIIDAYKNGGYPSLDGHYTVFGQVIEGM
                     DVVDAIANIETDSSDKPKEEVKIDKINILQEAKQK"
     gene            complement(2235..2675)
                     /gene="fabZ"
                     /locus_tag="ACG8KR_002550"
     CDS             complement(2235..2675)
                     /gene="fabZ"
                     /locus_tag="ACG8KR_002550"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HFQ0201864.1"
                     /translation="MSYVLSATEVMELIPNRYPICYIDYVDTLEPGKKIVATKNVTIN
                     EDFFQGHFPDNPVMPGALILETLAQAGSILILKSEEFLGKRAYIGGINKAKFRQKVVP
                     GDVLKCHFEITKMKGSVGTALSAAYVDDKKVCECEFTFIVNQNA"
     gene            2895..3650
                     /gene="fabI"
                     /locus_tag="ACG8KR_002551"
     CDS             2895..3650
                     /gene="fabI"
                     /locus_tag="ACG8KR_002551"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HFQ0201865.1"
                     /translation="MSFLEGKKIVIMGVANKKSIAWGCAEAMLKQGAEIIYTYQNERM
                     KKSLVKLVGEEAFTVECDVSSDESIEKAMNAIGEYAGEIHGLVHAVAYANKEELSGNV
                     SDISRDGYQLAQDISSYSLIAVSKYAQPFLAPNSGIVSMTYLGSERAIPNYNMMGIAK
                     ASLEAAIRYLAAEFSPKGIRVNGISAGAIKTLAVTGVKDYQKLIQLSESRTPDQQGVT
                     IEEVGNACAFLISPLSSGIIGDIIYVDKGVHLS"
     gene            complement(3760..4812)
                     /locus_tag="ACG8KR_002552"
     CDS             complement(3760..4812)
                     /locus_tag="ACG8KR_002552"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HFQ0201866.1"
                     /translation="MIKCGENNEGGKYMQIDWNNLGFSYIKTPWRFIAKWKDGQWEEG
                     ELTEDNYLTIHEGSPALHYGQQCFEGLKAYRRKDGKVNLFRPEQNSRRMNQSARRLLM
                     PMVPEKMFVDAVKQVVKANEDFVPPYGTGATLYLRPLLIGVGENIGVHPAPEYLFVVF
                     CTPVGAYFKGGLKPTNFIVSDYDRAAPKGTGAAKVGGNYAASLLPGTEAKELDYSDCV
                     YLDPATHTKIEEVGAANFFGITKDGKFITPQSPSILPSITKYSLLQLAEERLGLQAIE
                     GDIFIDDLDQFAEAGACGTAAVISPIGGIYHNNNLHVFYSETEVGPVTKRLYDELTGI
                     QFGDIEAPEGWIEVVE"
     gene            4938..5858
                     /locus_tag="ACG8KR_002553"
     CDS             4938..5858
                     /locus_tag="ACG8KR_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HFQ0201867.1"
                     /translation="MFSLLATAGTTDSSGETLTDQAVSKVNAFQRFWDGIDWDAILAA
                     FIQKSLYLIFLIVLFWILHRVGKYLIDKSFNQYSKKAILNESRLKTLHSLLNTIFHYT
                     LGFFFIYAILSAIGVPIGSLLAGAGIAGVAIGLGAQGFMSDVITGFFIIMEQQMDVGD
                     YIKLANLTIEGTVVSVGIRTLQLKATDGTVHFIPNRNITTISNLSRANMQVLLDIRIV
                     PEEGYDKIYEIIDRVNRTLAEKYQEELQTEPSIFGLVDLGNSNFAIRTVCYALNGKQY
                     ALKEEFLSSYVKELTASGFTIPNSPIAVGK"
BASE COUNT         1830 a         1197 c         1076 g         1886 t
ORIGIN      
        1 cctataaaaa aacaaaataa aaaagcagaa ataactggct atttctagcg gattatttct
       61 gcaataacta attatctatt ttttattttc aattaattta aagcttgacc atggtttcaa
      121 aaagtcaagg aggaataatt cgtcatcgga gattcttcct acaacattga cacgtccgtc
      181 gtttttcata tccttcaagg cgatttgtgt ttctcctttg tattgaccgt acccttcatt
      241 gtcaatcagt acatccccat gatgcatatc tcgcgtatta tgaggcggga aatctttatc
      301 tttgtagtat acacgagtca tcgttgaacg aaggatatat tctgagcgat cccctcgata
      361 actatgaagg ttatcaaaca agcagattcg ttcgttttct gtaatacctt cttccgtatc
      421 tactttcaat gttggataat ccgcgttaaa tgcttctgcc atttctttca attcttcttc
      481 acttgcgtac gcatttccaa tagaaatatc atcaatcaat cctgtaagga ttaaatgctt
      541 cacttgtgtc gcaatttcaa gatctcggtg gtcttccagc gtgcacaagc catcttgcgt
      601 aggccaaggg ccaaactcag cagattgcga attgacgaat gccatcgtat tcaaattata
      661 ttttctgaat ttttctgaac aaaaaacaaa gtgttcgtat cctaaaccag agtatcgatg
      721 aggataaaaa ttatgagaac ctaataaatt atccgtgttt ggcgaataac tcatgatgtt
      781 gtcaacgtaa ttcgtgcccg aactcatatt gatctcgatt ttgataccaa aaggattgcg
      841 cgtcattttt gcttcttctg ctcctgtgaa tccgatatct aagcggaccc cgtaggcacc
      901 catcttatgg aaaaaagata agtcatcgta agaaatctcc aactgttcaa ataacgctgg
      961 attgatatca accattactt ccatgcctag gctattcgcg taatcaacta cttctttaaa
     1021 ttctgaaaga actttttctt tatcatcatt gatctgcagc aaactggtaa aaacccgttt
     1081 atacccatac ttatgagcta aatccaagta cgctttgtcc ttttcaaatg ttgaccgttc
     1141 tggataaatc gagattccta attttcccat attaccattt cctcctgcat cttttcgttt
     1201 ttgtatccac ttacattata gcaaacacaa agtaaatggt ctacaccttt tctttttctt
     1261 ttaaaataaa tgataaattc caataattta agtaggtatt ttccctctat tttgttatca
     1321 ttaaagataa caaaatagta aattggaaag gaacagttat gaagaaaaat aaagttattt
     1381 taagtattag tttagcagca gcattgctgt ttgctgctgg ttgttctgca aacaacacga
     1441 cttcttctag ttcgtctaca gaaagttcaa aaagtacatc tactgcggaa agcacgactg
     1501 ccagtagcgt cgatttgaac gcattggaat taccacaatt gacgaaagat gtcgccgaaa
     1561 atgaagatct ggtcgaaatg gtgacgaata aaggcacaat caaaatcaaa ttatttcccg
     1621 aacacgcacc aaaagcggtt gaaaacttta tgacacatgc aaaagacggt tactacaacg
     1681 gactgacttt ccatcgcgtc atcaaagatt tcatgatcca aggtggcgat cctaaaggcg
     1741 atggcactgg aggggaaagc atttggggag aaggatttga aacagagatc aacaaccacc
     1801 tttataatat aagaggtgcg ttatctatgg ctcgctcaca agatcctaac agtaacggca
     1861 gccagttctt tatcgttcaa aatacagatg atatgcatga tggtctttta aaagacgact
     1921 atcctcaagc aatcatcgac gcttataaaa acggcggtta tccttcacta gatggccatt
     1981 atactgtctt cggccaagtg attgaaggaa tggatgtcgt ggatgctatt gcaaatatcg
     2041 aaacagatag ttcagacaaa ccaaaagaag aagtaaaaat cgataaaatc aatatactgc
     2101 aagaagcaaa acaaaaataa aaaaataacg tctgaatcct gcatagatga caacgtatgc
     2161 taggattcag gcgtcttctt tttacataaa aagcaagtaa gcttcctttt gttttgctat
     2221 atcaggtttt atagtcaagc attttggttg acgatgaacg tgaactcgca ctcacagact
     2281 tttttatcat ctacatatgc tgcagataac gctgtaccga ctgatccctt cattttggtg
     2341 atttcaaaat gacatttcag cacatctcct ggaacaactt tttgacggaa tttggcctta
     2401 ttgatcccac cgatataagc tctttttcct agaaactctt ctgattttag gataagtatc
     2461 gaacctgctt gtgccagtgt ttctagaatc aacgcacccg gcatcactgg attgtcagga
     2521 aagtgacctt ggaaaaaatc ttcattgatt gttacatttt ttgtcgcaac gatctttttt
     2581 cctggttcta aagtatctac ataatcaata taacaaatag gataacgatt aggaatcaat
     2641 tccattactt cagtagcaga taatacgtaa gacataatta aactccttct ttcaggcaat
     2701 tgccattttc attactttga ctatcaaagt atcatattcc taaatattta gcaatctttt
     2761 tttaatttaa gaaaaaatag tttgacaatc aaaacatttt gactaataat taagaaatca
     2821 agaagttatt ttagctatta ttctctattt ttttacgact atcaaatttt tatcagaaac
     2881 aaaggagagt cattatgtca tttctagaag gaaaaaaaat cgtcattatg ggcgtcgcta
     2941 acaaaaaaag cattgcttgg ggttgtgctg aagcaatgtt gaaacaaggg gcagaaatta
     3001 tctatactta ccaaaatgaa cgaatgaaaa aatcattagt caaattggtc ggcgaagaag
     3061 cattcactgt agaatgtgat gtttctagcg acgaaagcat cgaaaaagca atgaatgcca
     3121 tcggtgaata tgctggggag atccacggcc ttgtccatgc agttgcttac gccaacaaag
     3181 aagaattgtc cggaaatgtg tctgatattt ccagagacgg ctatcaatta gctcaagata
     3241 tcagcagcta ctctttaatt gctgtatcaa aatacgcaca accgtttctt gcaccaaaca
     3301 gcgggatcgt tagcatgact tatctaggtt cagaacgcgc aatcccgaat tataacatga
     3361 tgggtattgc gaaagcttct cttgaagctg ctatccgtta tttggcagct gagttctcgc
     3421 ctaaaggaat tcgggtcaat ggaatctcag ctggggcaat caaaacatta gctgtcacag
     3481 gtgttaaaga ctatcaaaaa ctgatccagt tatctgaatc tcgtacgcct gatcagcaag
     3541 gtgtgacgat cgaagaagtc ggtaatgctt gtgctttctt aatcagccca ctttccagcg
     3601 gaatcatcgg tgatattatc tacgttgaca aaggtgtcca tctttcataa acttttgttg
     3661 cattctttaa agtaaaacat ataaaaatca ggaaaaaact ttttaaagtc tcttcctgat
     3721 tttttctttt tacgtttttt tcaactcatt actttgaaat tattccacga cttcaatcca
     3781 gccttctggt gcttctatgt ctccaaattg aatacctgtc aattcatcat acaaccgttt
     3841 tgttactggt cctacttccg tttcactata aaatacatga agattgttat tgtgataaat
     3901 cccaccgatt ggagaaataa cagccgctgt accacatgca ccagcttctg caaattgatc
     3961 tagatcatca atgaaaatat ctccttcaat tgcttgcagt cctagtcgtt cttcagctaa
     4021 ctgcaacaag gaatatttcg tgatacttgg taagatagag ggcgattgcg gagtgatgaa
     4081 ttttccgtct tttgtaattc caaagaaatt tgctgcacct acttcttcga ttttcgtatg
     4141 tgtggctgga tcaagataaa cacaatctga gtaatccagt tcttttgctt ctgttcctgg
     4201 aagtaaactc gctgcgtaat ttccgccgac ttttgctgca ccagtcccct taggagcagc
     4261 acgatcgtaa tcagatacaa taaaattcgt tggttttagt ccacctttga aatacgctcc
     4321 tactggcgta cagaagacga caaataaata ttctggagct ggatggacac caatgttttc
     4381 accgacccca atcagtaaag ggcgcaagta taatgtagcg cctgttccat agggcggcac
     4441 aaaatcttca tttgctttta ccacttgctt gacagcatca acgaacatct tttcagggac
     4501 cattggcatc agcaatcgtc tagcactttg attcatccgt cgactatttt gttcaggtcg
     4561 aaagagattg accttgccgt cttttcgacg ataagctttt agaccttcaa aacattgctg
     4621 accataatga agcgctggag agccttcatg aatcgtcagg taattatctt ctgtcaattc
     4681 tccttcttcc cattgtccgt ctttccattt ggcaataaaa cgccaaggag ttttgatata
     4741 agaaaaacct agattgttcc aatcgatttg catatactta ccaccttcgt tattttctcc
     4801 acattttatc attatccata ttatttgtca ttttattttc agcttttttc cttcattata
     4861 gttatttttg ctatttcctt ttttgaaaac ttcttggtac aatacagaaa gaatcaaatt
     4921 ttagaaaaga ggattttatg ttttcattat tagcaactgc cggtacgaca gactcttctg
     4981 gcgagacatt gacagatcaa gctgtcagca aagtaaatgc gtttcagcgg ttttgggacg
     5041 ggattgattg ggacgccatt ttggctgcct ttatccaaaa aagtttatat ctgatttttt
     5101 tgatcgtcct tttttggatt cttcaccgtg tgggaaaata tctgattgat aagtctttta
     5161 atcagtattc taaaaaagcc attctaaacg agagccgctt aaagacttta catagtcttt
     5221 taaatactat ttttcattat acccttggtt tcttttttat ctatgcaatt ttatctgcta
     5281 tcggtgtgcc gatcggatca cttttggctg gtgccggaat tgcaggtgtg gcaatcggtc
     5341 ttggtgccca agggttcatg agtgatgtta ttaccggatt ttttattatc atggaacaac
     5401 agatggatgt tggggactac atcaagctag caaacttaac gattgaagga accgtcgtat
     5461 ctgttggtat ccgtaccctt cagctgaaag caacggatgg tactgtccat ttcatcccga
     5521 atcgaaacat caccacgatc agcaatctct ctcgtgcaaa tatgcaagta cttttagata
     5581 ttcggattgt tcctgaagaa ggatatgaca aaatctatga gatcattgat cgagtaaatc
     5641 gtacattagc tgaaaaatac caagaagagc tccaaactga accttctatc tttggattag
     5701 tggatcttgg aaacagcaat tttgcgattc gtacggtctg ctacgcattg aacggaaaac
     5761 aatatgcctt aaaagaagag ttcttgagct cctatgtcaa agaattgaca gcttctggtt
     5821 tcacgattcc gaacagtcca attgctgtcg gaaaataatt tggacaaacg atgcaacgaa
     5881 tctcactaaa aagagcgtgg gacaaaagta aagaacactt ttgtcccacg ctctaaacac
     5941 gtataaacgg cggaagcaga agcaactcct tcggaaataa gctgaaatt
//
LOCUS       DAZKLD010000151         5914 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.152, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000151 DAZKLD010000000
VERSION     DAZKLD010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5914)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5914)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5914
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.152"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(321..1103)
                     /locus_tag="ACG8KR_002554"
     CDS             complement(321..1103)
                     /locus_tag="ACG8KR_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0201868.1"
                     /translation="MENSSNSAEVNVSTSTSEVVKCSQETTSLPEELLGDWQINNKDA
                     GIEISDSKAVFRIGRSQVESKDYYVDVNNKMLLHVYFSFEYDSVKYIEVSIERTGYSE
                     FISKSVGILTMHTKDGQYISLGVKRVSGENNETEAVTEQTMDERIDSSKSNETEVDTG
                     KTIDERIDSFIETYTAIPPTWKLVNEDKGITINTDTEISTDAQSLSDLLANLTGISNA
                     VTEQVGKDIPLRLLENGKKDYSVVFKNGQVTDYNEVFKKVIE"
     gene            complement(1168..2004)
                     /locus_tag="ACG8KR_002555"
     CDS             complement(1168..2004)
                     /locus_tag="ACG8KR_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFQ0201869.1"
                     /translation="MRVIKTLNNSLILCLDKQGKEVILMGKGIGFQLKKGDLVPADKI
                     QKVFKLDSLSDALKKDYIKLFEAAPDDLIICVKRIIDYANAKFDFKLNKSLFFTLVDH
                     LKHAIERSQKGIVFQNRLLIEIQKYYPKEFEIGCYAIQLINKKMNVQLPEEEAGNIAF
                     HIVNAQSEQANMEETMLSVRALKDILAMLGYLFPEKKVDKDSMLYLRFVTHLQFFIAR
                     MIKKEELPEKDDFLLASVKQRFPKEYRAALKIKEYVQEEMGASANQDELLYLTLHISR
                     VY"
     gene            complement(2022..3431)
                     /locus_tag="ACG8KR_002556"
     CDS             complement(2022..3431)
                     /locus_tag="ACG8KR_002556"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010757186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFQ0201870.1"
                     /translation="MQYKQLSAFPEKFLWGASISAYQAEGAYQEDGKQPSIIDKYQHT
                     EGVANFEVASDFYHHYKEDIQLFKELGLKAFRFSIAWTRIMDIENNKTNQKGISFYHQ
                     VIDECLNQGIEPIVTMYHFDLPYFLEEQGGWLNRATIDYFVDYVQVLLTEYGDKVNYW
                     LTINEQNTMILHPGAIGLPPSGELPSKKELFQINHHVLLAQARVIHLYHQLNLKGKIG
                     PAINLTAMYQATSSPEDAIAAHNWETLRGWSFLDVAVRGKYNYLFENYLNDRGLYPKI
                     EAEDQAILSSGKPDFIAINYYSTATIAASKNDGSDVSARAGDQQIMLGEEGVYRAAEN
                     PYVDKTPYGWVVDPTGLRLTLRKLYDRYDLPILITENGYGAPDIVEEDRRINDQDRID
                     YLAKHILAIQAALTDGVDVFGYLPWSAIDVVSTHQGFNKRYGFIYVDRTDENLKELKR
                     IKKESFFWYQKVIARNGLA"
     gene            complement(3445..5268)
                     /locus_tag="ACG8KR_002557"
     CDS             complement(3445..5268)
                     /locus_tag="ACG8KR_002557"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFQ0201871.1"
                     /translation="MDYREVGRNVLQQVGGKENVVSLTHCATRLRFELKDKRKADTKA
                     LEKTLGVISVVDSGGQYQVVIGNEVQTAFKEIQKEVGTSIKKGPSSDNQESIISRLIS
                     VISTTFTPMIPAITGAGMVKAILAVLTLTGVLSDSSQTYIILNTIADAAFYFMPVLLA
                     YGAAIKFDVNPILAITVAGILLHPNISQLFSTGDAVSFLGIPVLAADYAGSVLPIIFT
                     VWIMSYVEPFADRISPSFIKFFTKPMLVFLIMGPLALVVIGPFGTLLNDLIAMGAEWI
                     NGKAAWLIPFLMGGLQPFLVVTGTAWAMTPIATMQLSSTGSEMINGPGMLASNIAQSG
                     ATFAVAAKTKNKELKQLAVSTGITALMGITEPSLYGVTLKLKKPLVASMIGGAIAGLY
                     AGLSGLVRYAFVSPGLAALPAFIGENPMNIVHAIITCVIAFVATFIIAYLIGFDDTSD
                     ESEQTAIAEAVIVENPVKGTVCPISEVNDAVFSKGLLGKGVAIHPEENSIYSPVEGEV
                     ITILESKHAIGLRSKEGIELLIHVGIDTVNLKGEPFEVFVKAGDQVSAGQLLINADFA
                     AIRNAGYDDTVIVVVTNSENYAGISEASGSLKEHEPLMQIS"
BASE COUNT         1828 a         1279 c          956 g         1851 t
ORIGIN      
        1 atctttagaa ccatttcaga tacatatgaa acaattacat atgatttttt cataaaaaaa
       61 cagtactgtc tagtagtata aaatatacaa atttcttaaa aattttttca ctaaaatcac
      121 tacctgaaac agtctaaaac acttatatat caacgtttaa tgaaactatc tccgtttcat
      181 ttcagataca aataaatata gtcgggctcc aaatccgaaa aaaaataccc ccagtagctt
      241 atcctaagct actggagatg tataagtacg gtcatataac aacggggtag ggtaaataaa
      301 tgaaaaagat ggaagaatca ttattcaata acttttttaa atacttcgtt gtaatcagtt
      361 acctgtccgt tcttaaatac cacagaataa tcttttttcc cattttcaag taaccgaagt
      421 ggaatgtctt taccaacttg ctctgtcact gcattactga tacctgttag attagctaat
      481 aaatctgata aagattgagc atctgtagaa atctctgtat cagtatttat cgtgattcct
      541 ttatcttcat ttacaagctt ccaagtaggt ggtatagctg tgtatgtttc gatgaatgaa
      601 tcaattcttt catctatcgt cttcccagta tctacttctg tctcattact cttagatgaa
      661 tcgattcttt catccatcgt ctgctccgta actgcttctg tctcattatt ctcaccggaa
      721 actctcttca cacccaaact tatatattgt ccatctttag tgtgcatagt taaaattccc
      781 acgcttttac taataaattc actataacca gttctttcaa tagagacttc aatgtatttc
      841 acactatcat actcaaaaga aaaatagaca tgtaaaagca ttttgttgtt tacatctaca
      901 taataatcct ttgactctac ttgagacctt cctatacgga atacagcttt actgtctgat
      961 atctctatac cagcatcctt attattaatc tgccaatctc caagtagctc ttctggcaag
     1021 ctagtagttt cttgtgaaca ctttaccact tcactggtag aggtcgaaac attcacctcc
     1081 gcactgttag aagaattttc caaggaatca ggagccttag cacaaccaac aagtctttat
     1141 atattgtgca gccgatattt tcttcattca gtacaccctt gaaatatgca gtgttagata
     1201 aagcagttcg tcctgatttg ctgaggcacc catctcttct tgaacgtact ccttgatttt
     1261 caaggccgcc cgatactctt tcggaaaacg ctgcttcact gaagccagca aaaaatcatc
     1321 cttctccggc aactcttcct tcttaatcat tcgggcaata aaaaactgta aatgcgtcac
     1381 aaatcttaag taaagcatcg aatctttatc aacctttttt tcgggaaaca gatatcctag
     1441 catagccaag atatccttta gtgctcttac cgagagcatg gtttcttcca tatttgcttg
     1501 ttcactttgg gcatttacaa tgtgaaacgc aatgtttcca gcctcctctt ctggcaactg
     1561 aacattcatt tttttgttga tcaattggat agcatagcag ccgatctcaa attcttttgg
     1621 gtaatacttt tgaatttcaa tcagcaggcg attttgaaaa acaatgcctt tttgactgcg
     1681 ttcaatggca tgcttcaagt gatcaaccaa ggtaaaaaat agacttttat tcagcttaaa
     1741 gtcaaacttg gcgttggcat aatcgataat tcgcttgacg caaataatta gatcatccgg
     1801 agcagcctca aacagtttaa tgtaatcctt ttttagagca tcagaaagtg aatctaattt
     1861 aaatactttt tggattttgt cagccggaac caaatcgcct ttctttagtt gaaatccaat
     1921 ccctttcccc atcaaaatta cttctttgcc ttgcttatct aagcaaagaa ttaaactgtt
     1981 gtttaacgtt ttaattactc tcattagatt caccgttctc tctaagctaa gccatttctt
     2041 gcgattactt tttgatacca aaagaagctt tcttttttta ttcgttttaa ttctttaaga
     2101 ttctcatcgg ttcggtcaac atatataaaa ccataacgct tattgaagcc ttgatgggta
     2161 ctaacaacat caatcgctga ccaaggtaaa taaccaaaaa catctacacc atctgttaaa
     2221 gctgcttgaa tagccagaat atgtttggct agataatcaa ttcgatcctg gtcattaatt
     2281 cgtctatctt cctcaactat gtcgggtgcc ccatagccat tttcagtaat taaaataggt
     2341 aaatcatagc gatcgtacaa tttcctcaat gttaatcgta gtccagtagg gtcaactacc
     2401 cagccatatg gcgttttatc tacataagga ttttcagctg cccggtagac accttcttct
     2461 cccaacatga tctgttgatc ccccgctcta gcggaaacat ctgatccgtc atttttgctg
     2521 gcagcgatgg tggcagttga ataataatta atcgcaataa aatcgggttt accggaggat
     2581 aaaattgcct gatcctctgc ctcaatttta ggatataatc cacgatcatt caaatagttt
     2641 tcaaacaaat aattgtattt tccccgaact gctacatcta agaaagacca gcctctcaat
     2701 gtttcccaat tatgggctgc aattgcatct tctggactgg aggtagcttg atacatagcc
     2761 gttaaattga ttgctggacc gatttttcct ttaagattca actgatgata caagtgaatt
     2821 actctagctt gagctaataa aacatggtga ttaatttgaa agagttcttt ttttgaaggc
     2881 agctctccac ttggtggcaa accaatcgct ccaggatgaa ggatcatcgt gttttgctca
     2941 ttaattgtca gccaatagtt caccttgtca ccatactcag taagcaatac ctgtacatag
     3001 tccacaaaat aatcgatagt tgctcgattt aaccaaccgc cctgctcctc tagaaaataa
     3061 ggtaaatcga aatggtacat ggtgacaatc ggttcgattc cctgattcag gcattcatca
     3121 attacttgat gataaaaaga aattcccttt tgattggttt tattattttc aatatccata
     3181 attcgcgtcc aagcgatgga aaagcgaaaa gcttttaagc ctaattcttt aaacagctgt
     3241 atatcttctt tatagtgatg gtaaaaatcc gaggcaactt caaaattagc taccccttct
     3301 gtatgctgat acttgtcaat aatagacggt tgtttcccat cctcctgata ggcaccctct
     3361 gcctggtagg cactaatgga ggcaccccat aaaaacttct ctggaaatgc tgaaagctgt
     3421 ttgtattgca caaaaatcct ctccttaact aatttgcatc aatggttcgt gttcctttaa
     3481 gcttccgctt gcttcactaa ttcctgcgta attttcactg ttggtcacta ccacaatcac
     3541 tgtatcatcg tagccagcgt ttctaatggc agcgaaatcg gcgttgatta ataattgtcc
     3601 tgctgacacc tgatctcctg cttttacaaa tacttcaaag ggttcccctt ttaaattcac
     3661 ggtgtcgatc cccacatgga tcaataattc aataccttct ttagaacgta gaccaatcgc
     3721 atgtttagat tctaaaatag taatgacttc tccctcaaca ggtgaataaa tgctgttttc
     3781 ttctggatga atggctacgc cctttcccaa tagtccctta gaaaagacag catcgttcac
     3841 ttcagaaata ggacacacgg ttccttttac cggattttca acaatgactg cttcggctat
     3901 tgccgtttgc tctgattcat cagaagtatc gtcaaatcca attagataag caatgataaa
     3961 ggttgcaaca aaggcgatta cacacgtaat aattgcgtga acaatattca ttggattttc
     4021 tccaataaag gctggtaagg ctgctaagcc aggggaaaca aaggcatatc tgactaaacc
     4081 gctcaagcca gcatacaaac cagcaattgc accaccaatc atggaagcaa ctaatggttt
     4141 tttcagcttc aaggttactc catagagtga aggctcagta attcccatta aagcagtgat
     4201 cccagtggac acagctagct gcttcaactc tttgttttta gttttcgcgg ctacagcaaa
     4261 ggtagcacct gattgagcaa tattagaagc cagcattcct ggcccattga tcatttcaga
     4321 acccgtactg ctcaattgca tagtagcgat tggcgtcatt gcccaagctg tacccgtaac
     4381 caccaaaaat ggctgcaaac cacccatcaa aaatggaatt aaccaagctg ctttgccgtt
     4441 aatccattca gcccccattg ctataagatc gttcagcaac gttccaaacg gtccaatgac
     4501 aaccaacgct aaaggcccca taattaaaaa taccagcatc ggcttagtaa agaatttaat
     4561 aaacgaaggc gaaattcgat ccgcaaaggg ctctacatag gacatgatcc aaacagtaaa
     4621 gataatcggt aaaactgatc ccgcataatc tgcagctaat accggaattc ctagaaaaga
     4681 aacggcatcg ccagtagaaa aaagctgtga aatatttgga tgaagcaaaa tgccagcaac
     4741 agtaatcgcc aaaattgggt tgacatcaaa ctttatggct gccccatagg ctaatagtac
     4801 tggcataaaa taaaaggctg catcagcaat agtattcaaa atgatgtaag tttgactact
     4861 gtctgatagt acaccagtca aagtcaagac ggccaaaatg gcttttacca tcccggcacc
     4921 tgttatagct ggaatcatcg gtgtaaaggt ggtagaaatg acactaatca atcttgaaat
     4981 aatactttct tgattgtctg aagaggggcc ctttttaatt gaagttccca cttccttttg
     5041 aatttccttg aaggctgttt ggacttcatt gccaatcacc acttggtatt gtccaccgct
     5101 atccaccaca ctaattacac caagtgtttt ttctaaggct ttcgtatcgg ctttcctttt
     5161 atctttgagt tcaaaacgca gtctggtggc acaatgggtt aagctgacca cattttcttt
     5221 tcccccaacc tgttgcaata catttcttcc tacttctctg taatccatta ttctctctcc
     5281 ttaaagcagt aaaaaagacc aagtcaaacc acgccccaat caacattgaa gtgttagttt
     5341 gtcttggtct tgcctgcata atcagtaaca tgccaataac gttattttgc tgtaatccaa
     5401 taatagcaca ttatgatagc gcaatcaaca gggaatttaa ttttttatct tttcgtttga
     5461 ccgtataggg taatataaca gttagatcac atcaactagg gtatatctgc gtatacttac
     5521 tgctaaaata agtatatcta atattaacta taccagtaaa accattccta cataaccagc
     5581 gggaaattcc caaaatcgtg aaagcatcaa aaatgcatat aacggatcac acaacttcaa
     5641 caattctgct cttgggaaaa attgatgaat ttggtgagta aaggatggaa taaacagcat
     5701 tccaacaata taggcaacaa tagtgatact tgtttttttg gtaaaaatca gtaagcaata
     5761 gccaatttca atcattccga gatataaagg tatcgataga atgatttggg ctagtattct
     5821 taaaaattaa ctagcaaatg tttaaactga tttatttcta atctacatgt agtaagtatt
     5881 tcaattccac caattatcaa taaaaatacc aaac
//
LOCUS       DAZKLD010000152         5905 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.153, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000152 DAZKLD010000000
VERSION     DAZKLD010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5905)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5905)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5905
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.153"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..450)
                     /locus_tag="ACG8KR_002558"
     CDS             complement(<1..450)
                     /locus_tag="ACG8KR_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFQ0201872.1"
                     /translation="MKYKEMFVPSLIQLAVEVKNKEEAFLIAAQQLEKMGYVNENYLN
                     GLIAREREFPTGLITKYLNIALPHSECEYVEKPFVFVMQLKNPIYFYQMGDNQEMTVE
                     NLFFLGIKEPKEQVGLLQAFMNLFMDETFVENYKKQETPQNIYELITN"
     gene            674..1465
                     /locus_tag="ACG8KR_002559"
     CDS             674..1465
                     /locus_tag="ACG8KR_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFQ0201873.1"
                     /translation="MLKKERQEQIITLLEKDFYLTNSEIANKLGVSEMTIRRDVTELA
                     KKNKVKKLYGGVERKEPYNRELSTQEKIDTNVAEKKYIGQVMNSMIPDNSTIYVGAGT
                     TILYALPFIQKKNLFVITNSLIAFNYLKNHTSYRILLTGGEFSPITEEFIGEVAERSF
                     ESLNVDISFAATNGIFDNNVTTAQFIEGNIQNAALNAAKIKCIVADYSKLNKSDVYTF
                     RKLTDFDYLITDNHLSEQDFNHYSKYLTILKEPKNDTDNHDEPIH"
     gene            1431..2384
                     /locus_tag="ACG8KR_002560"
     CDS             1431..2384
                     /locus_tag="ACG8KR_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase family hexose kinase"
                     /protein_id="HFQ0201874.1"
                     /translation="MILTITMNPSIDSIYFTKHFELGTMTRFDSPVRYVGGKGINCGR
                     ASALLGSSVILTGFLGGYFGSMIQDMLKNENLFDLHFLPIAEESRGAVTIMHDDETQT
                     ELVEAGPEIADTTIKKLMNDIKLISKNNYLSVITINGSVNNPDPFLYAKLLQYIKEQI
                     DPSTYILMDISGQHLREVLASKYKPMFIKPNIHEFSDIIHQKITQKSDIKHHLKTNPL
                     FKDIDVIMISCGSYGAVVKVKEEFWNVEIPKIPIINTTGSGDATVGGFAHSLEQNYPL
                     KECVRYAMACGMSNAQQSEVGKVIPADVKNLAKSIKITEMK"
     gene            complement(2782..3756)
                     /locus_tag="ACG8KR_002561"
     CDS             complement(2782..3756)
                     /locus_tag="ACG8KR_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFQ0201875.1"
                     /translation="MNKELNITQEWDKVFPKSNQVNHRKVSFPNRFGITLVADLYEPK
                     EVSEKKLPAIAVSGPFGAVKEQSSGLYAQTMAERGFLTIAFDPSFTGESGGVPRYVAS
                     PDINTEDFQAAVDFLSVQKNVNPERIGIIGICGWGGMALNAAAIDTRVKATVVSTMYD
                     MTRVTANGYFDVMDENARYEMREELNAQRIIDFKQGDYARAGGVVDPLPEDAPQFVKD
                     YHAYYKTDRGYHVRSLNSNDGWNRTSGLSLMNAKLLHYIPEIRHAVLVIHGEKAHSRY
                     FGEDAFKELKGDNKELMIIPGASHTDLYDQTDIIPFEKMATFFEENLV"
     gene            3871..4305
                     /locus_tag="ACG8KR_002562"
     CDS             3871..4305
                     /locus_tag="ACG8KR_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFQ0201876.1"
                     /translation="MYSIGQVSEMFGLPVSTLRYYDKEGLFPDIQRVSGIRKFSENEL
                     EALRVIDCLKKSGLEIKDIKQFMEWSKEGSKTFEVRKELFEKQKEVVEKEIAKLERVL
                     AMLNYKSWYYEEAIKAGNEEAVLTMIPDDLPQHVKEAYVHSH"
     gene            complement(4430..5452)
                     /locus_tag="ACG8KR_002563"
     CDS             complement(4430..5452)
                     /locus_tag="ACG8KR_002563"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HFQ0201877.1"
                     /translation="MKKIGFLSFGHWMEQGSLVKSAQDAYLQSIDLAVEAEKIGVDGA
                     FFRVHHFAPQIGSPFPLLAAIGAKTSKIEIGTGLIDMRYENPYYMAENAGLTDIITQG
                     RVQLGVGRGSPEQVLDGWKYFGYDYSEEEIKEVTRERTLEFLELLEGKPFEEPNPQPM
                     FPQGSGKLRIEPYSEGLRQRIWWGAGSQQTAIWAAQHGMNLQSSTLVNYESHEPFHIQ
                     QANQLRAYKEAWKQAGHDFEPRTLVTRSIQPITTDLDRRLFGQDGSPQQDAVGYLAYH
                     RNPTIFGKTFAGEPDQLIKELAEDEAIKEADTVLVTIPNTLGVEYNMHLLETIVKDIA
                     PELGWR"
     gene            5844..>5905
                     /locus_tag="ACG8KR_002564"
     CDS             5844..>5905
                     /locus_tag="ACG8KR_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (thioredoxin)"
                     /protein_id="HFQ0201878.1"
                     /translation="MKKIYFLCTGNSCRSQIAEG"
BASE COUNT         1859 a         1113 c         1010 g         1923 t
ORIGIN      
        1 atttgtaata agttcgtata tgttttgcgg tgtttcttgt ttcttgtaat tttccacgaa
       61 tgtttcatcc atgaataaat tcataaacgc ttgaagtaat cctacttgtt cttttggttc
      121 tttgattcct aaaaaaaata gattttctac agtcatttcc tgattatccc ccatttgata
      181 aaaataaatt gggttcttca gctgcataac aaaaacaaat ggcttttcga catattcaca
      241 ttccgaatgt ggcagcgcga tatttaaata cttagtaatc aatccagtag gaaattctct
      301 ttctcttgca atcagtccat tcaaataatt ctcattgacg taccccattt tttctaattg
      361 ttgtgcagcg attaaaaaag cttcttcttt attttttact tctaccgcta attgaatcaa
      421 actaggaacg aacatctctt tatatttcat tccaacctcc tcctttttga ttaagtttat
      481 taatttattg ttctactaag ttcatctttg ttttacaaga acattatatt tctgttagtt
      541 tgttttgtca acgctttcaa aagaaattta ttttgttaaa tttgatctac ttgaaaataa
      601 aatcgtttat ctatataatt aagaagaaca aagtcgaaca aaaattgact tttatgttaa
      661 aggagtaatt catatgctga aaaaagaaag acaagaacaa atcattacat tattagagaa
      721 agatttttat ttaactaatt cagaaatcgc taacaaatta ggcgtatcag aaatgaccat
      781 acgccgagat gtcactgaat tagcaaaaaa aaataaagta aaaaagctat atggtggcgt
      841 tgaacgaaag gaaccttata atcgagaact atcgactcaa gaaaagattg atacgaatgt
      901 agccgaaaaa aaatatattg gtcaagtcat gaacagtatg attcccgata attctactat
      961 ttatgtcggt gctggaacaa cgatcctata tgcacttcct ttcattcaaa agaaaaatct
     1021 atttgtaatt acaaacagtt tgattgcttt caactattta aagaatcata caagttatcg
     1081 gattttactt actggtggcg aattttctcc tattacagaa gagttcattg gggaagttgc
     1141 agaaagatct tttgaaagtt tgaatgtcga tatctctttc gctgcaacaa acggcatatt
     1201 tgataacaat gtgacaaccg ctcaatttat tgagggaaat attcagaacg cagcgctcaa
     1261 tgcagcaaaa atcaaatgca ttgttgcaga ttattcaaaa ttaaataaat cagacgtcta
     1321 tacttttaga aaattaaccg attttgatta tctaataacc gataatcatt tatcagaaca
     1381 ggatttcaat cattattcaa aatacttaac aatattaaag gagcccaaaa atgatactga
     1441 caatcacgat gaacccatcc attgattcca tttattttac aaaacacttt gaattaggta
     1501 cgatgacacg ctttgattct ccggtacgct atgtaggcgg caagggaatc aattgcggac
     1561 gagcttctgc tctactaggt tcatcagtta ttttaacagg gtttttgggc ggttattttg
     1621 gcagtatgat tcaagatatg ctgaaaaatg aaaatttgtt tgatttgcat tttttaccta
     1681 ttgcagaaga atcaagaggt gccgtcacga tcatgcatga tgatgaaacg cagacagagc
     1741 tagtggaagc tggtcctgaa atagcagata ctactatcaa aaaattaatg aatgatatca
     1801 aactaatcag taaaaataat tacctttcag tcattacaat aaatggttct gttaacaatc
     1861 cagatccctt tttgtatgct aaattattac aatatatcaa agaacaaata gatccttcca
     1921 cctatatatt aatggatata tctggacaac atctccgtga agtcttagca agtaagtata
     1981 aaccgatgtt catcaaacct aatatccatg agttttcaga tatcatccat caaaaaatta
     2041 ctcaaaaaag cgacataaaa caccatctaa aaacaaatcc actatttaaa gatatcgatg
     2101 ttatcatgat ttcttgcggc tcgtatggtg cagtagtcaa agtaaaagaa gaattttgga
     2161 atgttgagat ccctaagatt cctatcatta atacaacagg ctctggggat gccactgttg
     2221 gaggttttgc tcattcatta gaacagaatt atcctttaaa agaatgtgta aggtacgcaa
     2281 tggcttgcgg catgtcgaat gcacagcaat cagaagtcgg gaaagttatt ccagctgatg
     2341 taaaaaattt agcaaaaagc attaaaatca cagaaatgaa ataaatagta caaagtaaat
     2401 aattgtttat atgaaatgag acacaaagag actgtgacaa aaaacgtgtg taccgacccc
     2461 caaaagttag attttttggt ctaacttttg ggagtcgata cattgtcaca gtcctttttt
     2521 cgaataaacg gtgttctcct gccaactcct cggtcacaag tcacaatatt caacaaacat
     2581 gagaagctat tttttgaaat tgctccttgt gactcagagt taaacggcag gctcacgacc
     2641 tcttggtata aacggtgttc aaaagcttgc atctacgcta atgagtccaa ccaagtcaaa
     2701 aatgaggagc ttttttggct gtccttatta cttgatgcag ggcagttttt tcataatctc
     2761 ttttagatta aatccttttg gttacacaag gttctcttca aagaatgttg ccattttttc
     2821 aaatggaatg atgtctgtct ggtcgtatag atctgtatgg gatgctcctg gaatgatcat
     2881 gagttccttg ttgtcacctt ttagttcttt gaaagcgtcc tccccaaagt aacgtgaatg
     2941 agctttttcc ccgtgaatga ctaagactgc atgacggatt tctggaatat aatgcaatag
     3001 ttttgcattc ataagtgaca accctgatgt cctattccaa ccatcattgg agtttaaaga
     3061 acggacatga tatccgcggt ctgttttata atacgcatga tagtctttga caaattgagg
     3121 agcatcttca ggcaacggat caaccacacc tccagcacgt gcgtagtccc cttgtttgaa
     3181 atcaatgatg cgttgtgcat ttaattcttc acgcatttca tatcgggcat tttcatccat
     3241 aacatcgaag tagccatttg ctgttactcg agtcatgtca tacattgtgg aaacaactgt
     3301 tgctttgacc cgcgtatcga tagctgctgc attcaatgcc atacctcccc atccacaaat
     3361 accaatgatt ccgattctct cagggtttac atttttttgt acagataaaa agtctactgc
     3421 cgcttggaaa tcttccgtat tgatatcagg cgaagcaaca tatcgaggta ctcctccgct
     3481 ttctcctgta aacgatggat caaacgcaat cgttaaaaag cctctttcag ccatcgtctg
     3541 cgcatataga ccagacgatt gttccttcac tgcaccaaat ggcccgctca cagcgattgc
     3601 cggcaatttc ttttcactta cttcttttgg ttcatataaa tcagcgacta atgtgatacc
     3661 aaaacgattc gggaagctca cctttcgatg attgacttga ttgctttttg gaaatacttt
     3721 gtcccattct tgtgtgatgt ttaattcttt attcatattc tagactctcc tttgatcaaa
     3781 attgacattt gtttttgaat gtattattat gataaaactt aaacttgact ttaggtcaag
     3841 gtaaaaattt tattttagga ggattttttt atgtattcca ttggacaagt gtctgaaatg
     3901 tttggattgc ctgtttctac tttacgttat tacgacaaag agggattatt tcctgatatc
     3961 cagcgtgttt ctggtattag aaaattttca gagaatgagt tagaggcttt aagagtcatt
     4021 gactgtttga aaaaatctgg tttagaaata aaagacatta agcaattcat ggaatggagc
     4081 aaagaaggca gtaagacatt tgaggttcgt aaagaactat ttgaaaaaca aaaagaagta
     4141 gtagaaaaag aaatagcaaa attagaaaga gtactcgcta tgctaaacta caaaagctgg
     4201 tactatgaag aagcaataaa agccggcaat gaagaagccg tattgacaat gattcctgat
     4261 gatttacccc aacatgtaaa agaagcttat gtacattctc attgaacaaa cggataactc
     4321 actctccttc tctatgaact tcatgaaaac atgaataaac acctctaact gattgttaga
     4381 agtgtttatt ttttcactca agtatcctgc acttacaaaa atatcctttt taccgccatc
     4441 ctaattctgg agcgatatct tttacgattg tttcaagcaa gtgcatgtta tattctaccc
     4501 ccaaagtatt aggaatcgtg acaagtactg tgtctgcttc tttgattgct tcatcttcag
     4561 ctagctcctt gattaattga tcaggttcac cagcaaaagt ttttccaaaa atcgttgggt
     4621 ttcggtgata agcaagatag ccgacagcat cttgttgcgg actaccgtct tgtccaaata
     4681 agcgtcgatc caaatcagtg gtgattggct gaatcgagcg agtaactaat gttcttggtt
     4741 caaaatcatg acctgcttgc ttccacgctt ctttatacgc acgtaattgg tttgcttgtt
     4801 ggatatggaa tggctcgtgg gattcgtaat tgactaatgt agatgattgc agattcattc
     4861 catgttgtgc tgcccagatt gccgtttgct gcgaaccagc tccccaccag attctttgtc
     4921 gtaatccttc tgaataaggt tctatccgta attttcctga tccttgtggg aacattggtt
     4981 gaggatttgg ttcttcaaat ggttttcctt caagtaattc caagaattca agggtgcgtt
     5041 cccgtgttac ctctttgatt tcttcttcac tgtaatcata accaaagtat ttccagccat
     5101 ctagaacttg ttcaggtgaa ccacgcccca ctcctagttg cactcttcct tgcgtgatga
     5161 tatctgtcaa tccggcattt tctgccatat aataagggtt ttcatatcgc atatcaataa
     5221 gtcctgtacc gatttcaatc ttacttgtct ttgcgccgat tgccgctagc aaaggaaacg
     5281 gcgacccaat ctgaggggca aaatgatgta cgcggaaaaa cgctccgtcc actccgattt
     5341 tttctgcttc caccgctaaa tcaatcgatt gcaaataagc atcttgagct gatttcacaa
     5401 gcgatccttg ctccatccaa tgtccaaagc ttaaaaagcc gatttttttc aaggtaatga
     5461 cctcctttaa acctacaaaa atggaaggtt ttttattttt aatatgaact gattctacta
     5521 ctttttttct taaaagacga ataattgttc tcgcctcaca atcaagaaat gtatcggctt
     5581 actacacccc tactttcttc atcatctgcc gtgtcttaat ctcttttaac agtctgttac
     5641 atcgttgatt tttgactcaa aatgattgtt tctaacatgt atttatggta aaaaagacaa
     5701 gattagttct ttgtacacat cgatttatac gctgaacaaa aagaaatata taaactaaga
     5761 aacccattgt tttttcgctc catcacatag agtttacttt agataaacag tgacatttac
     5821 taatccataa cggaggaaat caaatgaaaa aaatctattt cttatgtacg ggcaattcat
     5881 gccgcagtca aatagcagaa ggcta
//
LOCUS       DAZKLD010000153         5877 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.154, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000153 DAZKLD010000000
VERSION     DAZKLD010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5877)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5877)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5877
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.154"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            641..1375
                     /locus_tag="ACG8KR_002565"
     CDS             641..1375
                     /locus_tag="ACG8KR_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFQ0201879.1"
                     /translation="MQNTFQSKSLYYQLVEILKQRIENSMVAHDKLPSERELTSFYGV
                     SRTTVRLALQELEKMGYIYRQHGKGTFVSELRTNATDLAGAYSFTEQMRAMGKHPETK
                     ILAFKKIEATKYLASHLNVQLGEPLFQLDRLRLADDLPMMIEKSYLPVKLFMSLSQEI
                     LFLKPLYNLFSENFNQIVRLADEEFYASIVQKEDSELLNIPEGSPVLHLVRSTYNLEN
                     EVIEFTLSVARADKFHYKIRHLRSTN"
     gene            1391..2560
                     /locus_tag="ACG8KR_002566"
     CDS             1391..2560
                     /locus_tag="ACG8KR_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFQ0201880.1"
                     /translation="MFMFSKEELRKLGAENTTREIRQQPELWHETFDRYQETVADLDR
                     LFTDAKQHAGERPIRVIFTGAGTSQYVGDTLIPFLTSQGNTNQFIFQSIGSTDIVATP
                     KEYLIPDETTILVSFARSGNSPESLAAVKMANQLVNKIYHIVITCAPDGLLATQMPTK
                     KENLVLLMPNKANDAGFAMTSSFSCMTLTALLIFDQASNQQKERYVHQLSRLGEEVIQ
                     RESEIQGYLASDFHRIIYLGSGSLGGLTREAQLKVLELTAGKIATVFDTSLGFRHGPK
                     SFVNEQTLVFLFVSNDSYTQKYDVDMLEEIKNDGIAQRVVGIGQKGSLFSGDSFELVA
                     DSLLPDGYLALADIMVAQTIALLCSVKVGNTPDTPSPSGTVNRVVKGVTIYPYEK"
     gene            2576..3571
                     /gene="lacD"
                     /locus_tag="ACG8KR_002567"
     CDS             2576..3571
                     /gene="lacD"
                     /locus_tag="ACG8KR_002567"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009025 - tagatose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HFQ0201881.1"
                     /translation="MLEWSENKRQAMERLSTPEGIITALAIDQRGALKKMMSALGMEA
                     TKEKIEQFKTLISQELTPYASAILLDPEYGLPAAQKRAAASGLLLAYEKTGYDQTVPG
                     RLPDLLSDCSVLRLKELGADAVKFLLYYDYEEAEEINRQKQVFVERIGSECMAEGLPF
                     YLELLSYDAKNVDTKSLSYAKAKPRKVIEMMKEFSKTQYKVDVLKVEVPVNMSFVEGN
                     ATGEIAYTRQEAAAYFKEQSDATDLPYIFLSAGVSAALFMETLRFAKHAGARFNGVLC
                     GRATWKDAVAPFVEKGEAATLEWLTEQGTQNIRQLNEVIRETAIPWYEKVSESTC"
     gene            3650..4576
                     /locus_tag="ACG8KR_002568"
     CDS             3650..4576
                     /locus_tag="ACG8KR_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HFQ0201882.1"
                     /translation="MNKRLLDYSASDFLKAKPMELKQAILASEGRTIMAENVPSSCPY
                     MGKSVTNAELERAAGADLILFNALDLFNPQISGVPLTLKENPIIWIKKALGRAIGVNL
                     EPVDKEVLMSENRTEISSGRIVSPETLKKANELGFNFICLTGNPGTGVSNRTIEKAIT
                     LSKSIFNGLIIAGKMHGAGVDEPIMNLDIAKRFIKTGVDILLVPAPYTVPYFMESDLR
                     GIVDYIKDFNRNKSIEEKVLVLTANGTSQDSSDQETIKKIALASKACGADIQHIGDSL
                     NGICLPENIFALGQAIRGYRHQIIMLGKSNYR"
     gene            4595..5845
                     /locus_tag="ACG8KR_002569"
     CDS             4595..5845
                     /locus_tag="ACG8KR_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFQ0201883.1"
                     /translation="MIDKVQKIAAKISNQRHLLALRDSMAAVMPLIIIGSFFMLLANF
                     PIEGFTKWLSEIGVQSFLNKASDSTFGIIGLAVTYSLGHNLAKTYGVDSLSAGLLSLS
                     GYVLLTPLLDADGSIGFPTKYFGTSGLFVGIIVALVSTEIFRWFVLKNITIKMPASVP
                     PNVSRAFVAIIPGFFVVLLWFIVVVICYKLGIENVHALIADTLAKPLSLLTKTLPGII
                     LVILIQCFFWMFGIHGAQVTGPIIEPLLLQNSDVNRIAYQAGKELPNIITYEFLYNFV
                     FSGGAGCLFALAILTFFFSKSQENKTLGKLSIAPVSFQVAEPLLFGFPTILNIKMLIP
                     FVTAPVVTTLITYFSMSMGLVAKPVGATIPWTTPPIIAGFLASGGRISGAVIQVITIA
                     INVLIYYPFFKLDDNAKLKSEKND"
BASE COUNT         1967 a          955 c         1204 g         1751 t
ORIGIN      
        1 gtttgaaaaa tgctatttcg aggtagaagg atattatata agcacggttg actaaacgaa
       61 gtgataataa aaaaagttat tcaagaataa gaaaaattat gatacgaatt cagataaact
      121 ggagaataca gagaccttta aaagggataa gaaacaacat aaattcctct taagagagaa
      181 cgaaaggaca aagccagcaa cttgatacac gagctagtat tagaggaatt tattctcata
      241 gactcccttt tgaatacgtt tcaactttaa ttttcatgtt cataaatgat ttgaaaccaa
      301 agcaaatgcg ttgcactttt cctccgtctt ccgaaaatcg ttcaattcac tggtaagtag
      361 tcatttgata gtttgaggca aattgttgat tgatgaaggg taggagctaa aaaaagatac
      421 cagttgaaga gagaaaaaga aaaaaataga gggtgttctt gaatgaaaaa agttggaaaa
      481 ttatttttca tttcctttta ttcctattaa agtcagtgat gtcaatcata ttaacaagac
      541 taaaaatgaa aataaaaaaa agattgttct tagtattgac tcataaaaaa taccactact
      601 atacttacat tgtggttata gccagttagg agagacatcg atgcaaaata cctttcaatc
      661 aaaatcactt tattatcagc tggtagaaat attgaaacaa cgaatcgaaa acagtatggt
      721 cgctcatgac aagctgccat cagaaagaga gttgaccagt ttttatggcg ttagtcgcac
      781 gacggtgcga ctagcattgc aagaactgga aaaaatgggc tacatttatc gacaacatgg
      841 aaaaggaacc tttgtttcgg aattaaggac aaatgcgact gatctagctg gcgcatatag
      901 ttttaccgaa caaatgcggg caatgggcaa acatcctgaa accaagatat tggccttcaa
      961 gaagattgaa gcaactaaat atttagcgag tcatctgaat gttcaactag gtgagccttt
     1021 atttcaatta gaccgattgc gcttagctga tgatttgcca atgatgatcg agaaatccta
     1081 tttgccagtg aagctattta tgtccctttc tcaagaaatt ctatttctga aacctttata
     1141 taatttgttt tcagagaatt tcaatcaaat cgtacggttg gctgacgagg aattttatgc
     1201 cagcattgtc cagaaagaag atagtgagtt gctaaatatt ccagaaggat caccagtgct
     1261 ccatttagtt cgctcaacct ataatctcga gaacgaagtg attgaattta cattaagtgt
     1321 agcgcgggct gataaatttc attataagat caggcatctg cgttccacta actgaattgg
     1381 aggaaagaaa atgtttatgt tctcaaaaga agaactacga aagctaggag cagaaaatac
     1441 tacccgagaa attcgacagc aaccggaact gtggcatgag acgtttgacc gttatcaaga
     1501 aacagttgca gatttagatc gtttgtttac ggatgcgaaa caacatgctg gagagcgacc
     1561 aatccgagtc atttttacag gggccggaac ctcgcaatat gtgggagata cactaattcc
     1621 gtttcttact agtcaaggaa atacgaacca gtttattttt caaagtattg gttcgaccga
     1681 tatcgttgct acgccaaaag aatacctgat tcctgatgag acaacgatct tggtctcatt
     1741 tgcccgtagt ggaaatagtc cagaaagtct agccgcagtt aagatggcca atcagctggt
     1801 caacaagatc tatcatatcg tgatcacctg tgcaccagac ggattattgg ctacacaaat
     1861 gcctacaaaa aaagaaaatc tagttttact aatgccaaac aaagccaatg acgctggttt
     1921 tgctatgaca agcagtttca gctgtatgac gctgactgcg ctacttattt ttgatcaagc
     1981 aagcaatcag caaaaagaaa gatatgttca tcaattaagc cgactaggag aagaagtcat
     2041 ccaacgagaa tcagagatcc aagggtatct ggcatcagac tttcatcgca tcatttactt
     2101 aggttcggga agcttaggcg gattgactag agaagcgcaa ttaaaagttc tcgagttaac
     2161 tgctggaaaa atcgcaaccg tatttgatac atctttagga ttcagacatg gtcccaaatc
     2221 ttttgtcaat gagcaaacat tggttttcct gtttgtcagc aatgactcat atacgcagaa
     2281 atacgatgta gacatgctgg aagaaatcaa aaatgacggt attgcccaac gcgttgttgg
     2341 aattggtcaa aagggttcat tgtttagtgg tgattccttt gaattagttg cagattcgtt
     2401 actgccggat ggctatcttg ctttagcaga tatcatggta gcccaaacga tcgcattact
     2461 ttgttcggtg aaggttggta atacaccaga tacaccatcg ccaagtggaa cagtcaatcg
     2521 tgtggtcaaa ggcgtaacga tttatccata tgaaaaatag gaaagagtgt gaagcatgtt
     2581 agaatggtcg gaaaataaaa gacaagcaat ggaacgtcta tcgacgccag aaggcattat
     2641 taccgctttg gcgatcgatc agcgaggcgc gctgaagaaa atgatgtcgg ctttagggat
     2701 ggaagcgaca aaggaaaaaa ttgagcagtt taaaacacta atttcccaag aattgactcc
     2761 ttatgcctca gcaattttat tggatcctga atacggactg cctgctgcac agaagcgtgc
     2821 agcagcgagc ggtctgctgt tggcatatga aaaaactggt tatgatcaaa ccgtacccgg
     2881 ccgcttgccg gatctgctgt cggactgttc agttctgcga ttgaaagagt taggtgcgga
     2941 tgcggtgaaa tttttactgt attacgatta tgaagaagca gaggaaatca atcggcaaaa
     3001 gcaagttttt gttgaacgaa ttggaagcga atgcatggct gaaggactgc cattttattt
     3061 agagttattg tcatatgatg ctaaaaatgt agatacgaaa tcgctttcct atgccaaagc
     3121 aaaaccgcgc aaagtgatcg aaatgatgaa agaattctcg aaaacccagt ataaagtaga
     3181 tgtcttaaag gttgaagtac cagtaaacat gtcatttgtt gaaggaaatg caacagggga
     3241 gatcgcctat acacgtcaag aagctgccgc ttattttaaa gaacaaagcg atgcaacaga
     3301 tttgccgtat atcttcttaa gtgctggggt atcagcggca ttattcatgg agacattgcg
     3361 ttttgccaaa catgctgggg cacgattcaa cggtgtactc tgtggcagag caacttggaa
     3421 agatgcagtc gctcctttcg ttgaaaaagg agaagcggca actttagaat ggctgacaga
     3481 acaagggaca caaaacattc gtcaattgaa tgaagtgata agagaaactg ccatcccatg
     3541 gtatgagaaa gtaagtgaaa gcacttgtta atgtttctaa tatattagta gtgataagag
     3601 atatgtgtga ttataattta tcattattga aactgggagg atattttata tgaataaaag
     3661 attgttagat tattcagctt cagatttttt gaaagctaag ccgatggaat taaagcaggc
     3721 aattttggct tctgaaggta gaacaattat ggcagaaaat gtgccaagta gctgtcctta
     3781 catgggaaaa tcagtgacaa atgcagaatt ggagagagct gctggagctg atttaatttt
     3841 gtttaatgca ctagatcttt ttaatcctca aatttcaggt gtacctttaa ctctaaaaga
     3901 gaatccaata atctggatta aaaaagcttt aggaagagct attggtgtaa atttagaacc
     3961 tgtagacaaa gaagttttga tgagtgaaaa tcgaactgaa atttcaagtg gtagaatcgt
     4021 ttctcctgag actttaaaaa aggcaaatga attaggcttt aattttattt gtttaactgg
     4081 aaatccagga acaggagtat ctaatagaac tattgagaaa gcaataacat tatccaaatc
     4141 aatttttaat ggattaatta tcgctggaaa aatgcatggt gctggtgttg atgaaccaat
     4201 tatgaactta gatatagcaa aaagatttat taaaacaggt gtagatattt tattagttcc
     4261 tgctccatac actgttccgt atttcatgga aagtgatttg cgaggaatcg ttgattatat
     4321 aaaagatttt aatagaaata aatctattga ggaaaaagtt ctagtactaa cggctaatgg
     4381 aacaagccaa gactcttctg accaagaaac tattaaaaaa attgcattag catcaaaagc
     4441 atgtggagct gatattcaac acatagggga ttctttaaat ggtatttgtc ttccagaaaa
     4501 tatttttgct ttgggtcaag caattcgggg atatcgtcat caaattatta tgttaggaaa
     4561 atctaattat cgttaattaa ggaggaaatg aaagatgatc gacaaagttc aaaaaatagc
     4621 tgcaaaaatt agtaatcaaa gacatctact agctttaaga gacagcatgg ctgcagttat
     4681 gccattaatc attattggtt ccttttttat gttattagca aatttcccga tagaaggatt
     4741 tacaaaatgg ttatctgaaa ttggagtaca atcctttttg aacaaagcat cagattcaac
     4801 atttggtatt attggattag ctgttaccta ctctttggga cacaatctag caaagactta
     4861 tggtgtagac tcattatcag caggattatt atctttgagt ggatatgttt tacttacacc
     4921 tttattggat gcagatggtt ctataggatt tccaacaaaa tactttggta cctcgggtct
     4981 ttttgttggt attattgtag cacttgtatc tacagaaatt ttccggtggt ttgtacttaa
     5041 aaatattaca attaaaatgc cggcaagcgt tccaccaaat gtaagccgag ctttcgttgc
     5101 tattattcct ggcttttttg tcgttctact atggtttatt gttgttgtaa tttgttataa
     5161 acttggaatt gaaaatgttc atgctcttat tgcagataca ctcgcaaaac cactatcttt
     5221 actgacgaaa actttaccag gaattatatt ggttatactt attcaatgtt tcttttggat
     5281 gtttggtatt catggtgcac aggtaacggg gccaataatt gaaccgttat tattgcaaaa
     5341 ctctgatgta aatagaattg cttatcaagc tggaaaagaa cttcctaaca ttataactta
     5401 tgaattctta tataactttg ttttttctgg tggtgcaggt tgtttatttg cacttgccat
     5461 tttaacattt ttcttttcaa agagtcagga aaataaaact ttaggtaagt tatcaatcgc
     5521 tccagtaagt tttcaagtag cagagcccct tctatttggg tttccaacta tattaaatat
     5581 aaaaatgtta attccatttg taactgcacc agtagtaaca acattaatta cttatttttc
     5641 aatgagtatg ggattagttg caaaacctgt aggagcaaca atcccatgga caactccacc
     5701 gataattgcc ggatttttag cttctggtgg acgaataagt ggtgcagtga ttcaagtaat
     5761 aacaattgca ataaatgttt tgatatacta tccattcttt aaattagatg ataatgccaa
     5821 gctaaaaagc gaaaaaaatg attagaacta cctatatgtt tctaactgta gttaagt
//
LOCUS       DAZKLD010000154         5809 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.155, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000154 DAZKLD010000000
VERSION     DAZKLD010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5809
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.155"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            450..1604
                     /locus_tag="ACG8KR_002570"
     CDS             450..1604
                     /locus_tag="ACG8KR_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C47 family peptidase"
                     /protein_id="HFQ0201884.1"
                     /translation="MIILKPKNKIYLGLLFSLMIGIFSIGITNTYASENSNFNINSQP
                     IEEQAINFAKTDFEHSITSILLMENNTETDINYTLGSPFKMLDGENTANSSIFFPVFS
                     DADNTIKYVYTVKKNTNGEYSGSISQFLAKELQNLVDSNITDTTLFFQDNSIYYSDNG
                     QIKLLWKSPLSTEKNGVSSFTEKNDLISVNIKNKIKNFSKKQNFPAVKRVKSVSSGDN
                     FIMINWRIAETQTSFPWCAAYVEAAILCNKTDVRATSARAIMHYTYPNLNNSQIESKS
                     LNQDQIIKYANYCGVYPKKVARILNLSEVQTQLRKGNAIYMGTAGYGNYSNSRHALAL
                     FGWVNQGGYQTYYVWNPWYNYPTVISANAGPVTIPVPGGGFTWDNTITNW"
     gene            1577..2020
                     /locus_tag="ACG8KR_002571"
     CDS             1577..2020
                     /locus_tag="ACG8KR_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0201885.1"
                     /translation="MGQYHYKLVNKPIVLVMGLVLVLFLTSCSKKQEIQKTELIPFLG
                     TWQTKDHSERLVITNLNTSKNIEQYTILVKENNHKIQIIDKNLNINEQVFIYKATNNL
                     LTLRSNKKQLDYTFSLDSPKKLVFILTTNDGTEGTAKPIEFFKAD"
     gene            3143..4075
                     /locus_tag="ACG8KR_002572"
     CDS             3143..4075
                     /locus_tag="ACG8KR_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFQ0201886.1"
                     /translation="MANEIVKYKPELNTIPLRKFTPIEMNLFFSIVSRMRDKGNQKIR
                     FYFDQLKDLSAYKQTAGEVFIKDLTRTYDHLMDLRFGKKSNGGLTIERFTMFTDFEIH
                     GEADNPYVDIKVHEKALPLLNNLESWVRFSLTEFRDLNSSYAKTMFRLLKQYRTQGWA
                     EFSKEDFLELLDIPKSYRQTNINQFVLKPIKEELTPLFKGLTIRKKYGKGRGKPVIGY
                     RFTWKAEINHADDFSKGKQEDLRTKLFNIEHNGELTQEEKWRAKDRILDLPLGTHEAN
                     FKKQQQTEKEEVEKQTLSNELKQDLLGNLQNLFD"
     gene            4092..4607
                     /locus_tag="ACG8KR_002573"
     CDS             4092..4607
                     /locus_tag="ACG8KR_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HFQ0201887.1"
                     /translation="MADKTIKDLAEELGVSKTAINKKVSDENRKLWFAKIGNKFVINE
                     IGQKAIKSMFFTENENSNQQPVGEKSKTENHKNANQVSELELFFFLEKQLNQKDIQLQ
                     EKDEQLKQMQKLLDQQQQLTLQANQQIKKLHEQLQLTYAEESSEKEPLSVTEETQKGQ
                     NKEKKWWQFWQ"
     gene            4903..5184
                     /locus_tag="ACG8KR_002574"
     CDS             4903..5184
                     /locus_tag="ACG8KR_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000353845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0201888.1"
                     /translation="MDILVRNINPKIITRVDKRCQELTTKTGEKWSRNKYLKVLIESD
                     FDRQLMNYKKEQFDLLLEKFIDIQTKNTETLNDYVHTNNQLIETLIGGI"
     gene            5186..5602
                     /locus_tag="ACG8KR_002575"
     CDS             5186..5602
                     /locus_tag="ACG8KR_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0201889.1"
                     /translation="MRKTSVQKHVTLYPSHIDVLDSIIEKNPDINNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     EKQLNPSQRNQPKNRQKETSQTFTSKNFDPTNFLKR"
BASE COUNT         2228 a          961 c          930 g         1690 t
ORIGIN      
        1 aaaaaaaaag actgctcgaa agagcagtct caagcaaaaa ataaacgaac aaacgcaatt
       61 actcacgaac acaaacaaat acaataaaat acctaaataa tgaaaataag tgataaaaaa
      121 caagggtatt tatagtttaa caagcgctca taaaaataac aagtctttaa aaaatgacag
      181 ataaaaatgc acaaagaatc ggacacagat aaccgcgaaa aatcaagctt tagaaagttg
      241 tttttagcta gaccaatgaa aaatcacaaa gtccgcccct gtatccgttg gtagaggggc
      301 tacttggttt agtatacatt atgttaacta gctcgttaaa taacgattat gttcccaaaa
      361 caatatttta tgaactataa caaaaatttg ttatcgcata ttgtatattt taattatagg
      421 tgtatatcac ttaattttaa cacaaaggcg tgattatttt gaaacccaaa aataaaattt
      481 atctaggtct cctcttttcc ttgatgattg gaatttttag tataggaatt acaaatacct
      541 atgcttcaga aaattctaat ttcaatatca actcccaacc aattgaagaa caagcaatta
      601 attttgcgaa aactgatttt gaacactcca taacttcaat tcttttaatg gaaaataaca
      661 ctgaaactga tattaactat actcttggtt cgccatttaa aatgctcgat ggagaaaata
      721 cggcaaatag ttctattttc tttccagtat tttcagacgc tgataatacg attaaatatg
      781 tttataccgt caaaaaaaat acaaatggag aatattcagg aagtatttca caatttttag
      841 caaaagaact tcaaaatcta gtagatagca atataacaga tacaacctta ttttttcaag
      901 ataatagcat ttattattcc gataatggcc aaattaaact actttggaaa tcacctttaa
      961 gtacagaaaa aaatggtgtc tcttccttta cagaaaaaaa tgatcttata tccgtaaata
     1021 taaaaaataa aattaaaaat ttctctaaaa aacaaaattt tcctgcggtt aaacgtgtaa
     1081 aaagtgtctc ctccggagat aactttatta tgattaattg gcgtatagca gaaacccaaa
     1141 caagttttcc gtggtgcgct gcttacgtag aagctgctat cttatgtaat aaaacagatg
     1201 tacgcgctac ttcagctaga gccattatgc attataccta tcctaattta aacaacagtc
     1261 aaattgaatc aaaaagtctt aatcaagatc aaataataaa atatgcaaat tactgtggtg
     1321 tttatcctaa aaaagtagca cgaatactaa acctttcaga ggtccaaaca caactaagaa
     1381 aaggaaatgc catttatatg ggaactgctg gttatggaaa ttatagtaac agccgtcatg
     1441 cacttgcatt atttgggtgg gtcaatcaag gtggttatca aacttactat gtgtggaatc
     1501 cttggtataa ctacccaact gtaataagtg caaatgcagg gccagttaca attcctgtac
     1561 ctggaggtgg ttttacttgg gacaatacca ttacaaattg gtaaataaac ccattgtttt
     1621 agttatgggt ctagtgttag ttcttttctt aactagttgt tcaaaaaaac aagaaataca
     1681 aaagactgag ctgattcctt ttctaggaac ttggcaaact aaagatcact cagaacgtct
     1741 agtaatcact aatttaaata cttcaaaaaa tatagaacaa tataccatct tagtaaaaga
     1801 gaacaatcat aaaattcaaa tcattgataa aaatttaaat ataaatgaac aagttttcat
     1861 ttacaaagca acaaataatt tactaacact gcgctctaat aaaaaacaat tagattacac
     1921 ttttagtttg gattctccaa aaaagctcgt atttattttg acaacgaatg atgggacaga
     1981 agggacagct aaaccaattg aattttttaa agctgattga tgaatagttg tttgatatta
     2041 caaaacaata atttcaccga aggtgaaaca agcgaaactt tacaaaaagt ggctagcatt
     2101 tgctagcctt ttttgtattg cggagcgtga gttttgaggg ggattcaagg gggagtttct
     2161 cccctttgac aagcggttat ttgcaccaaa tacgaaggta tttggctagc aaatatagag
     2221 cttgccaaac cctttagtgt gttttgtaga aagggacctt atagtcactt tcacattaca
     2281 aacgatacgt cctatggacg tatcaaaagg gtgggactta tgagcaaatt aaagcaaaat
     2341 ttggctagca atccttctaa aaaatacacc tattgatcag agtcaaaata aatcagtttt
     2401 cgagtgagcg aatccgaatt tgcaaatctg aagctttgca aatgagtgtg ccggcttccg
     2461 taaaagtcta ggtaacatta gtcgatatat acataaggct ctaatttgcg ttttaaggcg
     2521 ttttaaacgt tctggtatgt aatttatacc aaagcgactt taaaacgcct taaaacgaat
     2581 tttataggtc ttgtaggacg tttcagggtt gttagggctt gccctgaccg cctgtaaggc
     2641 gctttaaaaa caagataagg gattttatct agacttgtct taaaacgccg tagatgagcg
     2701 ctcagagctt cttaaaggac tccttacgaa ctgtttttaa gcaaaggagc ctttaggcga
     2761 ctgcgcagtt caatgtgaga gcgtagcgat cacaccttta aagcgaccgt agggagcaga
     2821 ttcccagttt aggaaatttc gcgaacctgt ttggagaaaa aaagtaggaa atttttaggc
     2881 gggaaatttt gcccccctct tttataatct cttttataac ctctttttaa acctctttac
     2941 cgtcggctgt atccttactc tcccaaggcc tgtagctttt ttagggcatg aaattatctg
     3001 tttataggca tgaaattatc tgtttatagg catgaaatta tctgtttata ggcatgaaat
     3061 tatctgtttt aggtattgtt ttaagacata atatacctta aaattatgtc aataaaatta
     3121 gttagactgg agtatcaaat aaatggcaaa tgaaatcgta aaatataaac cagaattaaa
     3181 taccattcct ttaaggaaat ttactccaat agaaatgaac ttgttttttt ctattgtttc
     3241 tagaatgcgt gataaaggta atcaaaaaat acgcttttat ttcgatcagc ttaaagattt
     3301 aagtgcttat aagcaaactg ctggagaagt ctttataaaa gatttaacaa gaacatacga
     3361 tcatttaatg gatttacgtt ttggaaaaaa aagcaatggt ggattaacca tagaacgatt
     3421 tacaatgttt actgattttg aaattcatgg agaagccgat aacccttatg tggatataaa
     3481 agttcacgaa aaagctttgc ctttacttaa caatttagaa agttgggtac gtttctcttt
     3541 aacagaattt cgagatttaa atagcagtta tgctaaaaca atgtttagat tactaaaaca
     3601 ataccgaaca caaggatggg ctgaattttc caaggaagat tttttagaac tattagatat
     3661 tcctaaaagc tatagacaaa caaatataaa tcagtttgta ctcaaaccca taaaagaaga
     3721 actcacacca ttatttaaag gattaacgat tcgtaaaaaa tatggtaaag gccgaggcaa
     3781 acctgttatt ggttatcgtt ttacttggaa agctgaaatt aatcatgctg acgatttttc
     3841 aaaagggaag caagaagatt tacgtacaaa attatttaat attgaacaca atggagaatt
     3901 aacgcaagaa gaaaaatggc gcgctaaaga tcgaatacta gatcttccgt taggtactca
     3961 tgaagctaat tttaaaaaac aacaacaaac tgaaaaagaa gaagtggaaa aacaaacact
     4021 ttcaaacgaa cttaaacaag atttattagg aaatttacaa aacttattcg attaaagtat
     4081 aaggagtatt tatggcagat aaaacgatca aagaccttgc tgaagaatta ggagttagta
     4141 agacagctat taacaaaaaa gtttctgacg aaaatcgaaa actttggttt gcgaaaatcg
     4201 gaaacaagtt tgtgattaac gaaatcggtc aaaaagctat aaaatcaatg ttttttaccg
     4261 aaaacgaaaa ctcaaatcaa caaccagttg gcgaaaaatc gaaaaccgaa aaccataaaa
     4321 acgccaacca agtttccgaa ttagaactct tttttttcct agaaaaacaa ttaaatcaaa
     4381 aagatataca gctacaagaa aaagacgaac aattaaaaca aatgcaaaaa ttactcgacc
     4441 agcaacaaca actcacctta caagccaatc aacaaatcaa aaaacttcac gaacaactcc
     4501 agctaaccta tgcagaagaa tcttcagaaa aagaacccct ttctgttact gaagaaactc
     4561 aaaaaggaca aaacaaggag aaaaaatggt ggcaattctg gcaataaccc aacaattttt
     4621 attcataaaa attgttgggt tactttcttt ttttgctttt acaagcatag cgacatgatc
     4681 tcacgtcgct attttttaaa gagcataagg aaacgcggta tcactttctt acgaaaggtg
     4741 ctaggtaggc tgtgcctacc acgtttctaa gaaaaaaatt tgctatcact ttttcgccat
     4801 tgtggctagc ttatgtatag tatttcggta aaaaacagct atacaaatac tatatttgta
     4861 tgatatacat aaattataca cacataagaa aggacgtttt ctatggacat tttagttcga
     4921 aatatcaatc caaaaattat tacaagagta gataaacgtt gtcaggaact tacaaccaaa
     4981 acaggagaaa aatggagtcg aaataaatat ttaaaagtgc taatcgaaag tgattttgac
     5041 cgtcaactta tgaattacaa aaaagagcaa ttcgatttat tactcgaaaa atttatcgat
     5101 attcaaacaa aaaatactga aacattgaac gactatgttc atacaaacaa ccaactcatt
     5161 gaaacactta tcggaggaat ataacatgag aaaaacttct gttcaaaaac acgttaccct
     5221 ttatcccagt cacattgacg ttttagattc catcatcgaa aaaaatccag atatcaataa
     5281 ctactcggaa gccattcgct ttttatgtat gagttatgaa cctgatacca atacaacaac
     5341 taccagtaac caagccaaac taaatgcgat gggaaaagaa atttctatca tgaccgaaat
     5401 tatcgcagaa tttgctgatt tgtatttaaa agatacaggc gttcttatcg gtacaaactc
     5461 gaaagtatat caagaagtaa aagaaaaaat tgaaaaacag ctaaatccct cccaacgaaa
     5521 tcaaccaaag aatcgtcaaa aagaaacgag tcaaacattc acttctaaaa atttcgaccc
     5581 aaccaacttt ctaaaaagat aaaaaaataa accaggaaat ttttaatttc ctggtttatt
     5641 ttctagagag cggtcttcca ataagatttg caaatcgatc ccaatttgcg taaaaaaaaa
     5701 gctgctcgta cgagcagcta aacaagaaaa agtcagcttg ttttaagggt aaaataagtg
     5761 taacaaattt tgcgaatgca acgatctttt ttattatacg caaacaatc
//
LOCUS       DAZKLD010000155         5774 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.157, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000155 DAZKLD010000000
VERSION     DAZKLD010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5774)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5774)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5774
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.157"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(438..941)
                     /locus_tag="ACG8KR_002576"
     CDS             complement(438..941)
                     /locus_tag="ACG8KR_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFQ0201890.1"
                     /translation="MRYVSKFLLGIYLFFLLWLILFKLSFNLPQFLTYSYSNVNLVPF
                     STFSFENTTVLRETTYNLIVFFPFGVLLNVNFKRLSFSKKLGIIFLVSFLAELIQFLF
                     GIGVADITDLITNTTGGLIGLWAYQLLNKHLSTNKLDRLAIILGYILFLFLIVAFLFL
                     TRTVRLQ"
     gene            1300..2325
                     /locus_tag="ACG8KR_002577"
     CDS             1300..2325
                     /locus_tag="ACG8KR_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFQ0201891.1"
                     /translation="MFKTYFRFNKIQDNPFRIMNLMLLIFIVLWLGYINHDFLISSFS
                     SLGIFTFFYYQNIPMKYLLKRMFLIGCGLLVAFVLGILSTYVLWLEPFAVALVAFSSR
                     FVLRLFHISKPGGLFFAMLSAMGTSMQLPIAQLPIVSLYFFMGVVFALIAAVITKLLD
                     SRPEQTIEKATLKERFHEEPLVIIDSVFYSAALFLSVYVSHGLNLHNPYWLTLSCASI
                     LLAENLDAMKHRQVQYLIGSMGGLCVSAFLSFVPFTQLQTIFLITFLYGIAQFLVARN
                     YAVANIFLNPMALMLSTLIRGAYLISLIEYRFLGIVIGSFIGLGVAWVMTVGLQHYLT
                     VVRQKLE"
     gene            2648..3526
                     /locus_tag="ACG8KR_002578"
     CDS             2648..3526
                     /locus_tag="ACG8KR_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase domain-containing protein"
                     /protein_id="HFQ0201892.1"
                     /translation="MRFKDMKRGNLMKKISNEIQILAETLTLPKVVIEQMIVQAKNIL
                     DEVFEQYSHELMDPNKAKATAKKIDKVYEEEPFAALAIYLYAASQSWKKIYQPLKIPR
                     TIYLATMNAFTRFIHEHFQVTGSYRFDRGFWIWHYTSGLIFRIGELEYEMTYFPKGYT
                     DLRLEGKQCLSLHIPSDADLSQNKIRDSYESAIRFFELYFPDYHYQVMYTDTWLLSPN
                     LTKWLKKESKICLFAADYRLLSVDEQDDSGVPWIFGRVDAQIHDYPESTSLQRQAKEQ
                     LLAGEHIGSGLGILTS"
     gene            4002..4118
                     /locus_tag="ACG8KR_002579"
     CDS             4002..4118
                     /locus_tag="ACG8KR_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HFQ0201893.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKDVKRNSGKR"
     gene            5046..5384
                     /locus_tag="ACG8KR_002580"
     CDS             5046..5384
                     /locus_tag="ACG8KR_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0201894.1"
                     /translation="MTQPVSSEEHDNLFRATEEFKKYQENIVPETPLASEPITDIDGN
                     QIGWYIQYEVMVVNNDSIVKNTVGLELTPIEKVSSLLTYIYTDESDETEIILTDYSKI
                     ATDKKYILWM"
     gene            5478..5708
                     /locus_tag="ACG8KR_002581"
     CDS             5478..5708
                     /locus_tag="ACG8KR_002581"
                     /inference="COORDINATES: protein motif:HMM:NF042636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6568 family protein"
                     /protein_id="HFQ0201895.1"
                     /translation="MLVEKGKENIYYVNVAKVREDENEWKEFKSRYSINSTPTFTVYR
                     EGSIEKTVFWTKESGMSLAEVEEFLDYVSMQQ"
BASE COUNT         1958 a          866 c         1069 g         1881 t
ORIGIN      
        1 ttctaaaaac tactgttttt ttcctttgaa atgccttcaa aaagtaggat caggcagaat
       61 atactggcag aatcaaggag aagggagcct aaataaggag aaaaaccata gaaaatataa
      121 agtaattact tataaaacaa gctttttata gaaaaataat atacctgagt agggtataaa
      181 ttaagacgga attcctttct agattgagat attgactagg aagaatgtta agattttaag
      241 cctttaagct aaaaaaaaac cctttttgtt aaataagggg aatatagcct gtcagtttca
      301 aactgcaagt cgtcctgctg caataatata gtagattaga gatgaaaata gattttttta
      361 aaccaatgac tgaataaaaa gaccgtcata gatacacatt ggaatgtgtc tattacggtc
      421 tttttttacg aacgatttta ttgaagacgg acagttcggg tcaaaaataa aaaagctacg
      481 atcagaaata agaataaaat gtatcccaaa ataatggcta gacggtctaa cttatttgtc
      541 gatagatgtt tgtttagaag ttgataggcc cataaaccta tcagtcctcc tgttgtatta
      601 gttatcaaat cagtgatgtc cgctacgcca ataccgaata ggaattgaat caattctgcc
      661 aagaagctta caaggaaaat aatccctaat tttttagaaa aagaaagtct cttgaaattg
      721 acattcaaaa gcaccccaaa aggaaaaaag acaatcaagt tataagtggt ctcacgtaat
      781 accgtcgtat tttcaaaaga aaaagtagaa aaagggacga gattgacatt gctgtaagaa
      841 taagtgagaa attgtggaag attaaatgat agcttgaata aaatcaacca taatagaaag
      901 aataaataaa tccctaataa aaattttgat acataccgca tagacatctc ctccatttaa
      961 aaagtatacc atcgaaaacc gctgaaaaat cataaaaaag tatgaagttt aaggaccaac
     1021 gtagttatcg tacgaaaatt agaagctttt ttttgttggg aatccggttt ctttaacaaa
     1081 gctaagatag cgagttgctt ggaaaagttg ctatcttttt tctgtcaaaa cgactcggaa
     1141 tgttcgtttt ttgttctaat ttctccaagt tgtttacttt ttgatactaa gtttatgaaa
     1201 aatcaagaaa tagttgttat actaggtaat gctgacaaac gggattggca tttcatcttg
     1261 gttcagcttt ttttccaatt actaggaagg agggatagat tgttcaagac ttattttcgt
     1321 ttcaataaaa tacaagataa tccttttcgt atcatgaatc tgatgctact tatctttatt
     1381 gtattatggt taggttacat aaatcatgat tttttgattt caagtttttc atcattaggg
     1441 atctttactt ttttctatta tcaaaacata ccgatgaaat atttgctgaa acgtatgttt
     1501 ctgatcggtt gcggactgtt ggttgctttc gttttgggga tcttatcgac ttatgtttta
     1561 tggcttgaac catttgccgt agctttggtt gctttttcca gcagattcgt gttgcgatta
     1621 ttccatattt ctaaaccagg tggcttgttt tttgcgatgc tttctgccat gggaacgagc
     1681 atgcagctgc caatcgctca attaccgatc gtctcacttt atttttttat gggtgtcgtt
     1741 tttgccctga ttgcggcagt gattacaaaa ttactggatt cacggcctga gcagacaatt
     1801 gaaaaagcaa ctttaaaaga aagatttcac gaagagcctt tagtgatcat tgattctgtt
     1861 ttctacagcg ctgccttatt tctttccgtt tatgtcagtc acggcttgaa tctgcataat
     1921 ccttattggt tgaccttgtc atgtgcatcg attttgctag cagaaaactt ggatgcgatg
     1981 aagcatcgac aagtccaata tctgattgga tcgatgggtg ggttatgtgt gtcggcattc
     2041 ttgtcatttg tgccgtttac gcagttgcag accatttttc tgatcacttt tctatatgga
     2101 atcgcacaat ttctagttgc tagaaattat gcagttgcta atatttttct taatccgatg
     2161 gcgttgatgc tatctacact tattcgtgga gcatacttga tttctttgat cgaatatcgt
     2221 tttttaggga tcgtcatagg aagtttcatc ggtttaggtg ttgcgtgggt aatgacggtt
     2281 ggtttgcaac attatttaac ggttgttcgt caaaaattag aatagaagga caaatatatg
     2341 tgccaaaaga ggttatgaca agatttttgt cacaacctct aattcaaggt gttcaaaagc
     2401 cagattctca gcattaagtc aaattcacac aaaaatgtgg aaaggaactt tcaaaaaaat
     2461 tttatattta ttctatcaaa aaattttgtt tatgatggta actgaataaa ctttcctgag
     2521 atggtcaatg ctttccttga aagtcttaca gatattcttt cttcatagaa agtttaagtt
     2581 ttatagttag tgtacaataa aagtatgttt ataccatatg gtgtccggat aatcagtcat
     2641 ttaaacaatg aggttcaaag atatgaaaag gggaaatttg atgaagaaaa taagcaatga
     2701 aatccaaatt ctggcagaaa ctttaactct tcccaaagtc gttatagagc aaatgattgt
     2761 acaagcaaaa aacatccttg atgaggtgtt tgagcaatat agtcatgaac taatggatcc
     2821 taataaagca aaggcgactg caaagaaaat agacaaagtc tatgaagagg aaccttttgc
     2881 agcattagct atttatctat atgcagcttc tcaaagttgg aaaaaaattt atcaaccatt
     2941 gaagattcca agaactatct atttggcaac aatgaatgct tttactagat ttattcatga
     3001 acatttccaa gtaacaggaa gctatcgctt tgaccgtggt ttttggattt ggcattacac
     3061 tagtggcttg atctttcgga ttggtgaatt ggagtacgaa atgacatatt ttccgaaagg
     3121 gtacacggat ctaagattag aaggaaagca gtgcctttct cttcatattc cttccgatgc
     3181 agatttatct cagaataaga taagagatag ttacgaaagt gccattcgtt tttttgaact
     3241 ttattttcct gattaccatt accaagtgat gtacacagat acttggttac tatcacccaa
     3301 tcttactaaa tggttgaaaa aagagtcaaa aatctgtctt tttgcagccg attatcgatt
     3361 attatcagtt gatgaacagg acgatagtgg agtcccttgg atctttggac gagtagatgc
     3421 ccaaatacat gattatccag aaagtacgag tttacagcga caagcaaaag agcaattgct
     3481 tgcaggcgag cacattggct ctggcttagg tattcttact tcataagttt agtacttcga
     3541 aatagtttaa aagagcgtga gacaaaagta aagaacactt ttgtctcacg ctctaaacac
     3601 gtataaacgg cggaagcaga agcaactcct tcggaaataa gctgaaattc acaaaaattt
     3661 gaaaagcaat tttcgtgaat ttcttcttat ttcttggagt taaacgcttc tgtcccagcc
     3721 tcttttttag tgttctatcg aacacacagt acgttcaata ggtaaactag ctataacaat
     3781 agatattaaa gttaacggac aagagggcct tttttaggta gctaaaacgt aataatgaaa
     3841 atttatttcc agagatagga gtcaatcagc tagtctgatc gactgtttta acaagttgac
     3901 tttcatttta acttttgata ccatttaaaa cacaaatcgt aattgtttcg atttgtgttt
     3961 tttagcatga ctacaatcaa agaagaaagt gggtcgtcca tatggaaaaa acagatatct
     4021 caagtgcata ccgccgcttg aaaagcccaa atatcaaaac acgaaaacgt gcgctgaaga
     4081 tcatcaaaga tgtgaaacgg aatagtggga aaaggtaaga agaaggaaac ttaattaaaa
     4141 aaatagccga aaaacgtttt tcggctattt tcaataatcg gtttttgtgt aaggtttccg
     4201 aaagaactgc tgttgatttt ccatttttaa catatgataa attggtagaa tctttctttt
     4261 caaattgata ccattcccta attccagtta tatagttaac tacagaacca caaagcacgt
     4321 acgtttataa aagtattatc tgtagttatt tttcaattcc ctttcaacct cttattttga
     4381 ccagtagtat tgatcacttt accttttaac acctttgttc gtttttgaaa catttttagt
     4441 caagaatgga aacgtaacta cataatcaaa ggttttgcga aatatttcat gaaatatttc
     4501 atgtagaagc gagcaatacg gttgcaaata accattatcg tttaataagc agttacgaaa
     4561 gctcccaata tctctaacgt tcagctcact ccatttcttt tcttcaaaaa aatcagtgat
     4621 tcgtatctct ttctatttgt cgaaaaagtt aactttaaaa agtaacgccg aaaaaatatg
     4681 caaaaaaata aaaaagcagt tactattgct agaataacat caaggaataa taagttttgg
     4741 ttttcagata atagtcgata agttgtgtat tggaatttta gatacagtaa taaagtattt
     4801 ttcatgaaat tttgtgagaa tcactattac aagtatcttt aataaaatta atggttaagg
     4861 aggaacttaa agtagaatgt aaatgaatag ttgtagtcag tcgattgtga tactagactc
     4921 aaagttgcag gaataagttt ttcaatttgt gattggaaga taaaaaatga aaaagaaggg
     4981 ctttttttta tactttgttg attgtagtgt ttattttccc tctgtttagc caagtaacat
     5041 tttctatgac tcaaccagtt tcaagtgagg aacatgataa cctttttcgt gctacagaag
     5101 agtttaagaa atatcaggaa aatattgttc cggagactcc attagctagt gagccaatta
     5161 ctgatattga tggaaaccaa ataggatggt atatacagta cgaagttatg gtggtaaata
     5221 atgactcaat agtaaaaaat acagtaggct tagagctaac accgattgaa aaggtgagtt
     5281 cattgttaac gtatatttat actgatgaaa gtgacgagac tgagatcatt ttaacggatt
     5341 actcaaaaat tgctactgat aaaaagtata tattgtggat gtagacacta gtgaaatact
     5401 ttatgatata gaaattgatg aaaatttact agatgtagca caagaaatcg atcatcaaaa
     5461 gcaggaagct attcgatatg ctggtagaaa agggaaaaga aaatatttat tacgtgaatg
     5521 tagcaaaagt tagagaagat gaaaatgagt ggaaagaatt caagagtcgc tactcaatta
     5581 actcgactcc gacatttaca gtttacagag aaggatcgat agaaaaaact gttttttgga
     5641 ctaaagaaag tggaatgtct ttagctgaag tagaagagtt tttggattat gttagtatgc
     5701 aacagtaagg gagttcttat tatgaaaaat tttgacacat attatgttat tgttttttcg
     5761 atatctttgc tgtt
//
LOCUS       DAZKLD010000156         5730 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.158, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000156 DAZKLD010000000
VERSION     DAZKLD010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5730)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5730)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5730
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.158"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            147..1043
                     /locus_tag="ACG8KR_002582"
     CDS             147..1043
                     /locus_tag="ACG8KR_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HFQ0201896.1"
                     /translation="MNKNDVLLIAKNKSLYFFPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMDFSSSEPKTDLFFGATNNGRKERAEKYKQEFTKR
                     GVTTNYFILPLRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYQFYHSENIFLLQERDLDELPEFLHTPFQPIPSELLEFFDAENWAKR
                     FFTTDSAIYEQYEYDLSSVKNK"
     gene            complement(1186..2127)
                     /locus_tag="ACG8KR_002583"
     CDS             complement(1186..2127)
                     /locus_tag="ACG8KR_002583"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HFQ0201897.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVVLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNKHVDLIVALTKANQLDDMFRSIQSIITENTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEAKLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAEGM
                     VRTIVSEFASIAAKEGITLDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAVWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            2253..3374
                     /locus_tag="ACG8KR_002584"
     CDS             2253..3374
                     /locus_tag="ACG8KR_002584"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HFQ0201898.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALGGTWRTIDGMDLLFRQRKISYLLTNWQGKNTAVIESAQVLGLDLLDKP
                     TDQTIRLASSYALGELIKDTLLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTRKLHLDRRNVLVSKVKVIIAADVTNPYTGQQGAAWQFGRQKGGTDTTLAFLD
                     KQAEKIAQQIKDVWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFELVSQITGLEKQI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDRELEGLFDFLPIV
                     LSIQLGPIDLEEAMEHRQTLEKTEILGQTLSRFFNILPK"
     gene            3632..5134
                     /gene="eat(A)"
                     /locus_tag="ACG8KR_002585"
     CDS             3632..5134
                     /gene="eat(A)"
                     /locus_tag="ACG8KR_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection-like protein
                     Eat(A)"
                     /protein_id="HFQ0201899.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQAFVYFPQQTKDKERLTYYVLNEIMDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEIAKNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKKVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDALTMNSQVSHHKRLLSVENLQLGYENLLFEPVHF
                     TIEPHQRMAISGPNGAGKSSIIHYLLGTFNGKVVGEKSQPKHLTISYVRQNYEDNRGT
                     LAEFAKKNRVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYI
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLEKI"
     gene            5201..5728
                     /locus_tag="ACG8KR_002586"
     CDS             5201..5728
                     /locus_tag="ACG8KR_002586"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFQ0201900.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQALAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVVDRLNLPMTADERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECMEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELISYE"
BASE COUNT         1880 a         1016 c         1213 g         1621 t
ORIGIN      
        1 tattttaatg gaagaatgat ttatttcatc gtattttcat agaaatgtta aatttttata
       61 aaaataatag gaagcgtttt actggctttt tactatttaa acaggctatc ataggaaaaa
      121 gtgacagaga aagtgaggcg gaacagatga acaaaaacga cgtattgcta attgcgaaaa
      181 ataaatcact ttattttttt ccttccatcc gctctttaga tgtttggtcc ggaaaagatt
      241 tactacagtc ggaattcatt aaatcagctc gacaacagtt ggcaaattat cagctgatcg
      301 tttttttaga ttatggattt gatatggaga tggctcgacg cgtgcgtcca tttaccaatg
      361 caaaaatcgt gttatttttt tggaaccact ttaaagcaga acacgaggcg atgctgaagc
      421 aggcacaaga tgaaccggca atcgatgaga tttatcattt tgatttttta gaagcgaaag
      481 aacttggact aaaacataat agttcctttt attcgaaaca tatggatttt tcttctagcg
      541 aaccaaagac ggatctattt tttggtgcaa caaataacgg cagaaaagaa cgggcagaaa
      601 aatataagca ggaatttaca aaaagaggtg ttacgaccaa ttactttatt cttccgcttc
      661 gaggaaatga acaacctgga taccttactt atgaagaata tctgaaaaaa accggggaat
      721 cgagggggat tctagaactg ttgcgggaag ggcagcaagg agtgactttg cgaacgtttg
      781 aatctatgta ttttcaaaag aaactagtga cagataatca agccatagct cattatcagt
      841 tttatcattc tgaaaatatt tttttgcttc aagaacgtga tttagatgaa ttaccggaat
      901 ttttgcatac gccatttcaa ccaatcccat cagaactgtt ggaatttttt gatgcagaaa
      961 attgggcaaa acgttttttc actactgact ccgctattta tgaacaatat gaatacgatc
     1021 tttcctctgt taaaaataaa taaaagtgcc aaccaatcat agatgaaaat aaacaagcgc
     1081 aattcttatg gcaacatgag aattgtgttt ttctttttga actttcatgg gggtaaaaaa
     1141 atgagaacca gctgctgatc cagctgattc tcatcagaga gccttttatt ctgcgccaag
     1201 gattccttct tttgcatgga ctaattgggt caaaaaggca caatatggtg tagcaatatc
     1261 atatttttgt ccttttcgcc aaacggcacc attgatgtag tcgatttcag tcaaacgatg
     1321 gtttttgatc aggtcttggt acatagatgg gtaatgcaac ccgatgttgt caggatcata
     1381 agtagaagca atatgttggt acacttcttc ttgatctaat gtgattcctt cttttgctgc
     1441 gatagaagca aactcagata caatcgttcg aaccatgccc tctgccgctt tttgcgcacc
     1501 taattcagca atattacaat ccaatatcgt gcataatcca tttaatgttc cgtttacaca
     1561 agcttttcgc caaatggaat aacgaacatt atgactatag attggattca attttgcttc
     1621 ttgaaagact tctacaactt tttttgtaaa tgcttcgcct tctggttcca agttctctag
     1681 ttcgatttct ccatcaccta ataatgtgac ttttccggga ccagctagac cagctgtcca
     1741 catcgtgatc ccaaataaga tatttttctt tggtacatat ttttccagta catcttcatg
     1801 acctaatcca ttcagtaaac acaaaacata agtattctca gtaataatgg attggatcga
     1861 acgaaacata tcatctaatt gattcgcttt tgtcaaggct acgatcaaat ccacgtgttt
     1921 gttgctttcg atgatttctt ctggtgaata gattggcagt tttgcaacta cttctttgcc
     1981 attaaagtca gcaatcaatc catttgtacg aatggcttca atgtgagcag gccagcgatc
     2041 gatcaagacg acctcatttc cgctttgatg caacattaat ccgattcggc tgcccattgc
     2101 tccggctcca gctattgcga ttttcatgtt ttttctctcc tttattcaat gagttccctt
     2161 ttagtatata gaaaaacaga agaaaatagc attgttttgt ttgaaaaaag tgaaaaaaag
     2221 tgtcttactt aagaagaaag tgagggatat agatggatat tttggcggca atcgattcat
     2281 ttaaaggaag tgcttcttca tttgaattaa atcaggcagt tctaaatggt tgggaagacc
     2341 gtaaaggaag aaaaatcaat gtgccgattg cagatggcgg agaaaatact gcagaagcaa
     2401 tctattacgc gctcggggga acctggcgta cgattgatgg gatggatctc ttatttcgtc
     2461 agcgaaaaat cagttattta ttgacgaact ggcaaggaaa aaataccgca gtcattgaat
     2521 ctgcacaagt attaggatta gatttacttg ataaaccaac ggatcaaacc atccgacttg
     2581 cttctagtta tgccttggga gagcttatca aagatacact gcttcaagaa gtggaacaaa
     2641 tcattgtcac tttaggagga agcggatgta gcgatggtgg tctaggatta ctccaatctt
     2701 tgggtgcttc tttagaaggg tacacagaag gaaatccttt gttatctact agaaaacttc
     2761 atttggatcg aaggaacgtt cttgtttcta aagtaaaagt gatcatcgct gcagacgtga
     2821 ccaacccata tacaggtcaa caaggagctg cttggcaatt tggaagacaa aaaggaggaa
     2881 cggatacgac gttagctttc ttggataaac aagctgaaaa gattgctcag cagataaaag
     2941 atgtttggga ggtggattta gatgacatcc caggaagcgg agcagcaggc ggtattggcg
     3001 gagccctttt tttgttagga gcgaagatgg tctctggttt tgagttggtc agccaaatca
     3061 ctggattaga aaaacagatc gaacaagcgg atgtcatcat tacaggagaa gggcggattg
     3121 atcagcaaac aatccacgga aaagttccat atggcgtagc agtgcttgca aaaaaatatc
     3181 gaaaaccaat aatcgctatt tgcggaagta gagaccgaga gctggaagga ctctttgatt
     3241 ttctccccat tgtcttaagc attcaattag gaccaatcga tttagaggaa gcaatggaac
     3301 acaggcaaac attagaaaaa acagaaatct taggacagac actcagcaga tttttcaaca
     3361 ttctcccgaa atagttgaca aaaacaattt ctttatgcta tcgtaatttc aaatatagga
     3421 caaagtgagg gatccgtgca ttgaaaaatt aagtcgattg atgaaagctg cattcagact
     3481 gtaccttgtg gagacaaggg agcagtgtgc agattttcgg gattggcttg gttctttcga
     3541 tgtatgggtc tttttgtgtg ccccaaccct tgcatcctct ttagccagtt ctgaaaatag
     3601 gactggtctt tttttacgag gtgatggaat aatgtctaaa atcgaaataa aaaatctgac
     3661 attcggctat gacagccaag gcacattatt atttgaacaa gccaatctaa actttgacac
     3721 acaatggaaa ctaggactta tcggacgaaa cggtcgagga aagacaactt tactgaatat
     3781 cctgcaaaac aaactccctt atcaagggca agtgatccat cagcaagcgt ttgtctattt
     3841 cccgcagcaa acaaaagata aagagcgttt gacctattat gtgttaaatg agattatgga
     3901 ttttgagata tgggaaattg aaagagagct ccaactgatg caaacagatc ctgaaatctt
     3961 atggagagaa ttcagcacac tatcaggagg agagaagaca aaagtcctac tggcactctt
     4021 atttgtggat gacactcatt tcccgttaat cgatgaacca acgaatcatt tggatatctc
     4081 tggcagaaaa caagtagcgg cttacttgaa aaagaaaaaa caaggattca tcgtggtgag
     4141 tcatgatcgg ggatttatcg atgaagtagt ggaccatgtt ttagcaatcg aaaagagcca
     4201 actggaactt tatcaaggga atttctctat ctatgaagaa cagaaaaaac ttcgtgatga
     4261 atttgaaata gctaaaaatg aaaaattgaa aaaagaagtc agcaggctaa agaaaacagc
     4321 agctgaaaaa gcagaatggt ctcgttcccg agaaggggac aaaacaaaga aaaaagtcgg
     4381 attcatcgat acggaatcta ggcgagtgaa taaaggagca gtaggcgctg atgctgcacg
     4441 gacgatgaaa cgatctaaag caatcgtgaa tcggatggaa acccagatca gtgagaaaga
     4501 aaaattatta aaagatatcg agtatatcga tgctttgacg atgaatagcc aagtgtctca
     4561 tcataaacgg ctgttaagcg tagaaaatct tcaattaggg tatgaaaatc tgttattcga
     4621 gccagttcat tttacgatcg aacctcatca gcggatggca atttcaggtc ctaacggcgc
     4681 gggaaagtca tccattatcc attatcttct ggggacattc aacggcaagg ttgtaggaga
     4741 aaaatcccag cccaaacatt tgaccattag ttacgtacgc caaaattatg aagacaatcg
     4801 agggacattg gcggaattcg cgaagaaaaa ccgagtagac tatcaagcat ttttgaataa
     4861 tctccgaaag cttgggatgg aaagagatgt ttttcataat aaaatcgaac agatgagtat
     4921 gggacaacgg aaaaaagtgg aattggctaa atctttatcc caaccagctg agctctatat
     4981 atgggatgaa ccattgaatt atttggatgt cttcaatcaa gaacaattag aacaactgat
     5041 tttgaacgtg aaacctgcta tgttactagt ggaacatgat caaacctttc tagataaagt
     5101 atctactgag attatttctc ttgagaaaat ctaaaccttg taatctcaaa gacaacaagg
     5161 tatggttaag gagaactttg tcaacgaagg agagaatcac atgctttatg taacgagaca
     5221 cggagaaaca acttggaacg cacaaggatt agtttgcggg cgagcagatg tgccgctgac
     5281 tgaaaaagga cagatgcagg cacaggcact agcagaaaag gtagtagatt tgcctgttcc
     5341 aatcacgaaa atcatccatt cccctcttca acgagcaaga gatacagcac aagcagtagt
     5401 tgatcgtctc aatttgccga tgaccgctga tgagcgattg gtagaaatgg attttgggga
     5461 ttatgacgga ctgccaagca aagatgaaaa ctttcaaaaa gcgcgtttag cgtttgctgt
     5521 acgtttccca aatggagagt cggtcttaga tgtgtacgca cggatcgttc ctttattgaa
     5581 agaatgcatg gaagacgaag aaaatgtcta cttgttagtc tgtcataatg ccttgataag
     5641 agtgatcaat gcttattttc atccaatgcc taatgaaggg ttctttacat tcatggtaga
     5701 caatacagaa ttaatttctt atgagtaatt
//
LOCUS       DAZKLD010000157         5665 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.159, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000157 DAZKLD010000000
VERSION     DAZKLD010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5665)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5665)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5665
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.159"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(136..462)
                     /locus_tag="ACG8KR_002587"
     CDS             complement(136..462)
                     /locus_tag="ACG8KR_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0201901.1"
                     /translation="MNKVNMNKKFIIIVIVVLAIIGIIFSKMTGTSGLEKSSKPLVTQ
                     ILQEQYNLNRSCDDVTITHENGDNTYRAKAILDNGSAININIEYYPKKDRIYVEIPYA
                     EVLMLN"
     gene            complement(508..870)
                     /locus_tag="ACG8KR_002588"
     CDS             complement(508..870)
                     /locus_tag="ACG8KR_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010717431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HFQ0201902.1"
                     /translation="MYCRKCGKEITDESKYCQFCGALQETDETTGADAVKKSTEQTIN
                     EMKEQIVHGQETGKLYLIIGWISMVVALAFIPVVFGAVAVIMGYLYRDKDEQMGTILM
                     IAGVAGAIFGVLLGMSMY"
     gene            complement(907..1121)
                     /locus_tag="ACG8KR_002589"
                     /pseudo
     CDS             complement(907..1121)
                     /locus_tag="ACG8KR_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324259.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            1249..1584
                     /locus_tag="ACG8KR_002590"
     CDS             1249..1584
                     /locus_tag="ACG8KR_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFQ0201903.1"
                     /translation="MKQKQYAKVIREKRKALGWTQKELAEKIFSTQQAVARWENSVTE
                     PNLDSLTALSRALGTPVSHFLDNVVVDYEEEFLALYRSLSTEDAVRTIDYMKLLKRQE
                     NERNQLLKD"
     gene            complement(1660..3657)
                     /locus_tag="ACG8KR_002591"
     CDS             complement(1660..3657)
                     /locus_tag="ACG8KR_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0201904.1"
                     /translation="METSMINIPIYVDKEKLLEKPVTKKEKTDVRDRCLDNYREVTRD
                     EFVEILNSSQSFIPSKTKNKGNDKESFVETRVIILDVDNTVKDEKGKVVDLSKDDSRY
                     LSIEKVLSINLVHNSAFAIQKSIRYSENLEKYKIVFLLKEAITDYGKMVAVYEYLKKN
                     IPGCDDNVNASNRMFFGGYKSDAVIIINEDNMLDITELPIDFTMTCSKNCQSDSGREY
                     ELVNETDFVKLIKNDDKEEMKQWFKDCLFDSSDMSFNEIYERLLTIDMNKLLKSSKNL
                     RCLFHNDHNPSASIFTSKTGHSIFYCHSSNCDISENFIGVVMLILKKDSRVETFNWLL
                     ENLDLYPTHFRKLKEESKVLFDTLESMKDKLFRTIRNYLEEMRRVYDVLLSDVNFFDD
                     SKESVTCILSGEQLAKKMSFYYGKTYDIDKCNKLLSLMTFIGLIKKLDDEDVPLKMLE
                     YLNRLKEEKALGNFNHKRSNVYRLDVFNATSVIQKLNEDILPLLEKFHFTYQYFSYEW
                     VKICFNEKEAKRVFPQNSRSELSKEKQLILDEAHEVLQGLSWQGTYLMTEKELTNTVC
                     ERLRNVGNNSVENALKENRGYFVKQGFTLERASKDIKRLYNVKASSSFLVYLAKGAGI
                     DEVIKELILSGVELVPLYQFTSVTRDDGRRVRKRKDPIVLN"
     gene            complement(3755..4030)
                     /locus_tag="ACG8KR_002592"
     CDS             complement(3755..4030)
                     /locus_tag="ACG8KR_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0201905.1"
                     /translation="MSLQIIDSIVSIQVEQQVKEIITVLKPMLEDIISGVTLEYMDYQ
                     QASEYLGVSVGTIRKYVSQYGLPVIKIDTVIRFKKSDIDSFMERYKG"
     gene            4262..4996
                     /locus_tag="ACG8KR_002593"
     CDS             4262..4996
                     /locus_tag="ACG8KR_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFQ0201906.1"
                     /translation="MIKRPPINYLERKKILGTKIKAIRKSKKLTQPAFGLMINNGQLI
                     DKKTIYEWEKGTYLPIPERLSRIADLGNMSIEELVCGNVEEYILGIILYRDSIVLDGI
                     TFPDKNLFQHLRQQFPPVHSNLDTWLDRYSKLEPEMQEFIANKTCNKVKNEKISLFNI
                     LKIEELFINAIVEEFDNNILFLTSSIEELLERMVDEWLPIQLKDMSYPEEAVREITDN
                     INKLEQTISSIGKKYTKKKMKGGDTI"
     gene            5035..>5665
                     /locus_tag="ACG8KR_002594"
     CDS             5035..>5665
                     /locus_tag="ACG8KR_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFQ0201907.1"
                     /translation="MAKISKYKKKNGQVAWMYKGHIATDPRTGEKINTTRRGFSSKQE
                     AQKDYDEYRHQILYGVKKKAPDMTFEDLYNEWITHQRTSVKASTIAISVRYANNQILP
                     AFGKLKLSNISVPYCQKVVDEWHSKYESYDYMRKQTAQILRYGVAMQYIDNNPMEKTL
                     LPRKKEYEKNRKFYSKEELNNLLDAFKDFGNMKQYAFFRLLAYTGMRKSE"
BASE COUNT         1903 a         1046 c          804 g         1912 t
ORIGIN      
        1 aaaattcccg acaatcattg taatattttt ttgtcacctg tattagtatt taatttatag
       61 gaaactataa aaactcccta gtgaatttac acctttggga gttcctgttt ggacggtatg
      121 cataatacat ttaaatcaat ttaacattaa cacctcagca tatggtatct ccacatatat
      181 acggtctttc tttggatagt actcaatatt tatgttgata gcacttccat tgtctaatat
      241 agctttagcc cggtatgtgt tatctccatt ttcatgagta atcgtcacat catcacatga
      301 tcgatttaag ttgtactgtt cttgtaaaat ctgtgttacc aatggtttcg atgacttctc
      361 taatccagat gtacctgtca tttttgaaaa aatgatacct ataatagcca agacaacgat
      421 aactataatg ataaacttct tgttcatatt tactttattc aactttttat ctcctatctc
      481 caaaataatt tgctcttatc cctcttatca gtacattgac atccctaaaa gtacaccaaa
      541 gattgctcca gctacccctg ctatcatcaa aatcgtaccc atttgttcat ctttatctcg
      601 atataagtag cccataataa cagcaacagc accaaataca acaggaataa atgctaacgc
      661 tactaccata gaaatccaac caataatcaa atacagcttc cctgtctctt gtccatgtac
      721 gatttgttcc ttcatttcat taattgtctg ctcagtactt ttctttactg cgtctgctcc
      781 agttgtctca tctgtttctt gtagtgctcc gcaaaactgg caatacttac tttcatctgt
      841 tatttctttg ccacattttc tacaatacat gtcttctcct cctaaaaaca ttcaattgtt
      901 tattagttaa ctgtttactt tattaaatac atcagaaaac agctcattca ttggtacact
      961 taatatctca gaaagcatct tcatttgata gggtttagga atcgttctcc ctgtttgcca
     1021 cgaacttaca cattgctgtg taacattcat ttttttgcta actccgtttg agaaagtcct
     1081 ttttcttttc tgtatcggct gagattactt acttttttca tgattctccc ctcctgtttt
     1141 attatataca acaatatgat gtatttcaac tcataaaata taaaaaaact tcaaatacca
     1201 ttacaaatac aacaacttgt tgtactatac tgagatgggg gtgtattaat gaaacagaaa
     1261 caatatgcaa aggtcattag agaaaagcga aaagctctcg ggtggacaca aaaagaatta
     1321 gctgaaaaaa tcttctctac acaacaggca gtagcaagat gggaaaattc tgtaacagag
     1381 cctaacctcg atagcttaac cgctctttct agagccctag gaacccctgt aagccatttt
     1441 ctagacaatg ttgtcgtaga ttatgaggaa gagtttttgg ctctctatcg ttctcttagc
     1501 actgaagatg cagttcgaac aattgactat atgaagctat taaaaaggca agaaaatgaa
     1561 cgcaatcagc tattgaaaga ttgaaaaata aataacccac cgaactttaa cggtgggtgt
     1621 attgagatga aataagatta agagctatgg ataacctgtc tagtttaata caattgggtc
     1681 ttttctcttc cttactctcc gtccatcatc tcttgttaca cttgtaaatt gataaagagg
     1741 aactaattca actcctgata atatcaattc ttttattact tcatctatcc ctgctccttt
     1801 tgctaggtag actaaaaaac tactacttgc tttcacattg tatagtctct taatatcctt
     1861 agaagctcgt tcaagagtga atccttgttt aacaaagtag cctctgtttt cttttaaagc
     1921 attttccacg ctgttattcc caacattacg tagacgttca caaacagtgt tagtaagttc
     1981 tttctctgtc attagatatg ttccttgcca cgataagccc tgtaaaactt catgtgcttc
     2041 atctaaaatc aattgttttt ccttgctaag ttctgatcgg gaattttgag gaaatactct
     2101 tttagcctct ttttcgttaa aacatatctt aacccattca taagaaaaat attgatatgt
     2161 aaaatgaaat ttttccagaa gtggaaggat atcttcattc aatttttgaa tcacagaagt
     2221 tgcattaaaa acgtctaatc gatagacatt gcttctctta tgattaaagt ttcctaacgc
     2281 tttctcttcc tttaacctat ttaggtattc gagcatcttc aaaggaacat cttcatcatc
     2341 taattttttt atcaatccta taaaagtcat aagtgatagt aacttattac atttatctat
     2401 gtcataagtt ttaccatagt aaaaactcat ttttttagct aattgttccc ccgacaaaat
     2461 acaagtcaca ctttccttac tatcatcaaa aaaattaaca tcagaaagca agacatcata
     2521 gacacgtcgc atttcctcta gataattcct tattgtcctg aaaagtttgt ctttcattga
     2581 ttcaagagtg tcaaaaagta cttttgattc ctctttcaat tttcgaaaat gtgtaggata
     2641 caagtctagg ttctctaata gccaattaaa tgtttctacc cgactgtctt tctttaaaat
     2701 tagcatcaca actccaataa aattttcact gatatcacaa ttactggaat gacaataaaa
     2761 aatggaatgc ccagttttac tggtaaaaat actagcagag ggattatgat cgttgtgaaa
     2821 cagacatctt aagtttttac ttgacttaag taatttattc atatcaatgg ttaacaatct
     2881 ttcataaatc tcattaaaag acatgtcgga ggaatcaaac aggcagtctt taaaccactg
     2941 tttcatttct tctttgtcgt cattttttat caacttaaca aaatcggttt cattgactag
     3001 ttcatactct ctccctgaat cactttgaca atttttagaa catgtcatgg taaaatcaat
     3061 cggaagctca gtaatatcta acatgttgtc ttcattaatt attatcactg catctgactt
     3121 atatcctcca aaaaacattc tattcgaagc atttacattg tcatcacacc cggggatgtt
     3181 ctttttcaag tattcgtaaa cagcaaccat tttaccatag tcagtaattg cttcttttaa
     3241 tagaaagaca atcttgtatt tttctagatt ttcagagtac cgaatacttt tttgtatcgc
     3301 aaacgcagaa ttatgaacta aattgataga taaaaccttt tctatgctta agtatctact
     3361 atcatcttta ctcaaatcta ctaccttccc cttctcatct ttaacagtgt tatcgacatc
     3421 cagaataatt actctggttt ccacaaaact ctccttgtca ttgcctttat tctttgtctt
     3481 gcttggaata aatgattgtg atgaatttag tatctctaca aactcatctc ttgtaacttc
     3541 tctatagtta tctaaacacc tatctcttac atcagttttt tctttttttg taactggctt
     3601 ctctaataat ttttctttat ctacataaat tggaatatta atcattgatg tttccatttg
     3661 tgtttcctcc ttataaaatt ctgttaaaga ctaaaacata cagataataa aaacaaactc
     3721 tttattacta tatgttttag tcttatttgt ttactcaacc tttataccgt tccatgaaac
     3781 tatcaatatc agatttttta aatctaatga ccgtatcaat ttttataaca ggtaacccgt
     3841 attgagaaac atattttcta atcgttccta ctgatacacc tagatattca cttgcctgct
     3901 gatagtccat atactctaga gtgactccag aaataatgtc ttctagcata ggctttagca
     3961 ctgtaattat ctctttcact tgttgttcaa cttgtataga aacaatgcta tcaatgattt
     4021 gtagagacat atttccccct cctttcgtga ttgcttgaac aaattttcta agaattatct
     4081 tcgtattttt tgctcaaaat aaaagtattt tgttattaac cgattaatca aacaaaaaat
     4141 gattaccagt tcatcgacat acacataata cctcgataaa aaagaggtgt caatatcttt
     4201 gtaaaattta tttacaacac ccctttttcg aggtatacta attttaggag gtgtggtcaa
     4261 tatgataaaa cgtccaccaa taaattattt agaacgaaaa aagatactcg gaacaaaaat
     4321 aaaagctata agaaagagta aaaaattaac acaaccagca tttggtctta tgattaacaa
     4381 cggtcaatta attgacaaaa aaactatcta cgaatgggag aagggaacct atttaccaat
     4441 accagagcga ttatcccgaa ttgctgattt gggaaatatg agtatagagg agttagtttg
     4501 tggcaatgta gaagaatata ttttgggaat tattctttat agagatagca ttgtactaga
     4561 tggtataaca tttccagata aaaacctgtt ccagcatctt cgtcaacagt tcccaccagt
     4621 acactctaat ttagatacat ggctggatag atattccaaa ttagaaccag agatgcaaga
     4681 atttattgct aacaagactt gtaataaagt taaaaatgaa aaaattagtc ttttcaatat
     4741 cttaaaaata gaagaattat tcatcaatgc tattgtagaa gaatttgaca ataacattct
     4801 tttcctcact agcagtatag aagaactact agaaagaatg gtagatgaat ggctacctat
     4861 tcaattgaaa gacatgtctt atcccgaaga agctgtgaga gaaattactg ataatataaa
     4921 caagctagaa caaacgattt catcaattgg gaaaaaatac actaagaaaa aaatgaaggg
     4981 aggtgatacc atctagtggt aagtactcct tgtaaactaa gaggagatga tttcatggca
     5041 aaaatatcaa agtacaaaaa gaaaaacggt caagtggcgt ggatgtataa aggacacatt
     5101 gccaccgatc cacgaactgg tgaaaaaata aatactacga gaagaggttt tagtagcaaa
     5161 caagaagctc aaaaagatta tgacgagtac agacatcaaa tattatacgg tgttaaaaag
     5221 aaagcacctg atatgacatt tgaagacctg tataatgaat ggataactca tcagcgtacc
     5281 agtgttaaag cttcaacaat cgcaatctca gtaagatacg caaataatca aatacttcca
     5341 gcatttggta agcttaaact atcgaatatc tctgtcccat attgtcaaaa agtggtagat
     5401 gaatggcaca gtaaatatga aagttacgat tatatgagaa aacagactgc acaaatttta
     5461 cgctacgggg ttgcaatgca atatattgac aataatccta tggaaaaaac acttcttcca
     5521 cgaaaaaaag agtatgagaa aaatcgtaaa ttttactcaa aagaggagct taataattta
     5581 ctggatgctt ttaaagattt tggtaacatg aaacaatacg ccttcttcag attattggca
     5641 tacacaggta tgagaaagag tgaag
//
LOCUS       DAZKLD010000158         5646 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.160, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000158 DAZKLD010000000
VERSION     DAZKLD010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5646)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5646)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5646
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.160"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..287)
                     /locus_tag="ACG8KR_002595"
     CDS             complement(<1..287)
                     /locus_tag="ACG8KR_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0201908.1"
                     /translation="MTYKHLTIDELTMIESYYLQHNKPVEIANRMGRAIQTIYNVVNK
                     FKQGKTALDYWHQYKENKKKCGRKVIQLPAHEVDYIKEKVTLGWTPDVIIGR"
     gene            622..2013
                     /locus_tag="ACG8KR_002596"
     CDS             622..2013
                     /locus_tag="ACG8KR_002596"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HFQ0201909.1"
                     /translation="MNKNGEWNAARRIFIILMDVLVLNVSIYLSFLLKFQGEVPVRNL
                     ETFQHSAIFISIIFIALNILLGAYVFYNRMISDIVFITVIGQVLIALWIMVVTFAGRW
                     FAFPRGVILISFILGTILLSVYRIVVYKLYLRVSRDKKVLIVGLEKDVAPAIQNFQSK
                     KNNKHKVEAVAIDNYYENTKKLIDDMDIVYLASKIPEDEKINIYQLVTKKEKKLFLNT
                     TFENLTMINPNIMNFEDESIIEASGFRIQPEYELFKRLFDIIVSLILLILASPFMLIT
                     AILVKTTSPGPIIYKQVRITKNQKEFSIYKFRSMSATAEAKSGPVLAKSNDARVTPVG
                     KFIRAVRFDELPQIFNVLKGDMSIVGPRPERPFFVDQFNEENPYYYLRHNVRAGITGY
                     AQVYGKYVSDYNSKLRFDLLYIKKYSLLLDLKILLQTIKILFDKMSSQGLDEDENISS
                     LAEIDFSQEKIYR"
     gene            2072..2875
                     /locus_tag="ACG8KR_002597"
     CDS             2072..2875
                     /locus_tag="ACG8KR_002597"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFQ0201910.1"
                     /translation="MLLSLYDKEKPEYLKEALTSIFEQTVMPKEIVLVYDGPINEKLQ
                     VVVTYFQQKDPHLFTVVKLEKNQGLGIALAIGLEHTKNRLVARMDTDDIMEPQRLEKQ
                     LEIFQKDPEIAIVGSNIEEFTGTLSNVIGKRIVPEYNNAIRVFSKRRNPFNHMTVMFD
                     KKAILEVGNYQPLSGFEDYYLWARLLKAGYKGYNIQETLVYARTGKDMYARRGGMKYL
                     GSGLMGRYRIWKDGLGSVKDFIIVSCGHIFISLLPNQLRGKFYQTQLRK"
     gene            2908..3762
                     /locus_tag="ACG8KR_002598"
     CDS             2908..3762
                     /locus_tag="ACG8KR_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LicD family protein"
                     /protein_id="HFQ0201911.1"
                     /translation="MKKIQQITLKMSQEIVDFCLKNQLLCYFCGGGAIGTVREQGFIP
                     WDDDLDFFMPREDYEKFYQLWVNSEKSKKYPIQKASKNYNDHNSFTTIRDADTTFIKT
                     YQRDLDIVHGITIDIFPLDVAPVSKFSRKIQKLWALVYALFCSQVVPEKHGGIMATGS
                     KILLSIFRSPRTRYKIWSFAEKQMTKYNNEPTGYVTELCVGPKYMGNVYPAKDFESAV
                     WVPFEDTEMPVPVGYDNYLSQVFGDYMQLPPEKDQVSHHEAVYIDPEHPYTMYKGKYY
                     LTKGAEKK"
     gene            3759..4460
                     /locus_tag="ACG8KR_002599"
     CDS             3759..4460
                     /locus_tag="ACG8KR_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HFQ0201912.1"
                     /translation="MITAIIIAGGVGKRMGQDIPKQFITIEGKPIIIYTLESFQHHPQ
                     VDRILVVCKSGWEETMWAYIKEFNISKVKWVITGGSKGQESINNGVQFLKDYSEDDDT
                     IIIHDGIRPLVDELVLSDVIVKCKEFGNGVTSLPYNEQIFIKKTEETTEQYVDRNTLR
                     RVSTPQAYQYGKLRAAYDRAVKEDIGMTDSSYTNTMMVDLGETLYFAAGSDKNIKLTT
                     TDDLELFKAYLKMKD"
     gene            4468..5535
                     /locus_tag="ACG8KR_002600"
     CDS             4468..5535
                     /locus_tag="ACG8KR_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFQ0201913.1"
                     /translation="MMTLIKQPMYQEDLAKVINHTPKIKQLHGKSVLMIGASGMIGSF
                     LIDTLMAANEQLGIKIKVYAMGRNRQKLEKRFASYLELDTFEIVEGDVTEPLPKEIQA
                     DYLIHGASNTHPKAYATDPIGTIMTNLAGTEQVLKHAVATQPVRVLFLSTVEIYGENR
                     GDIEKFTEDYCGYIDCNTLRAGYPEGKRVSESLCQAYIAKHDLDIVIPRLCRTFGPTM
                     LLSDTKASSQFILNAVEQKDIVLKSEGNQYFSYVYVGDAVSAILHLLLEGEKGEAYNV
                     SSENFDLRLKELAQQLADISGKQVIFELPDEIEQKGFSKASTAVLENKKIKETGWKPI
                     FPLNESLVHTVELVKGEKYNV"
     gene            5528..>5646
                     /locus_tag="ACG8KR_002601"
     CDS             5528..>5646
                     /locus_tag="ACG8KR_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFQ0201914.1"
                     /translation="MCEISIIVPVYKVEKYLRKCVDSILAQTFTDFEVILVDDG"
BASE COUNT         1945 a          869 c         1074 g         1758 t
ORIGIN      
        1 cgcccgataa tgacgtcagg cgtccaacca agagtgactt tctctttaat gtaatctact
       61 tcatgagcag gtaattgaat gacttttcta ccacattttt tcttattttc tttatactgg
      121 tgccaataat caagagcagt cttgccttgc ttgaacttat tgactacatt ataaatagtt
      181 tgtatagcac gtcccattcg gttagcgatt tcaaccggct tattatgttg aagataatat
      241 gattctatca ttgtcagttc gtctatggta agatgtttgt aggtcattta tggttacact
      301 cctttgtttt ctttcgtcgg aaatacaatt tgagtgtacc ataaatgcct ttttattttt
      361 ctaacttaat tttacaattc gcgttatatg aatattgtaa cttgtgaata aggaattagc
      421 aggagaatac tagttcagca taaaggaagt gatgggactt ctgagacaac tttctgttat
      481 gcagaaatac acatataaac agacaataaa cgcttttccc cgtttctttt aatttccttt
      541 acaatttggt tattcactag gtttttaaac tttgctatgg taagatgata gaggcattat
      601 tttatggtta ggagtgtata tatgaataaa aatggggagt ggaatgcggc acgcagaata
      661 ttcattattc ttatggatgt acttgtatta aatgtatcga tctatcttag ttttttgctg
      721 aaattccagg gagaagtacc agtgagaaac ttagaaacct ttcagcattc agcaattttt
      781 atttccatca tctttattgc gttgaatatt ttgttaggag cttatgtatt ctataaccga
      841 atgatcagtg atattgtctt cattactgtt attggacaag tactaatagc attgtggatc
      901 atggtagtta cttttgctgg ccgatggttt gcatttccgc gtggcgtcat tttgatctcg
      961 tttatcctag gtacgatatt acttagtgtt taccggatag tcgtttacaa actgtactta
     1021 agagtcagtc gtgataagaa agtccttatc gtgggtttgg agaaagatgt cgcaccagct
     1081 atccaaaatt ttcaatctaa gaaaaacaat aaacataagg ttgaagcagt ggcaatcgat
     1141 aattattacg aaaatacaaa aaaattgatc gatgatatgg atatcgtcta tttggcttct
     1201 aaaatcccag aagatgaaaa aatcaacatt tatcaattag tgactaaaaa agagaagaaa
     1261 ttatttttaa atacgacttt tgagaatctg acaatgataa atccgaatat tatgaatttt
     1321 gaagatgaaa gcatcattga agcttctggg ttcagaatcc aaccagaata cgaactattc
     1381 aagcgtctct ttgatatcat cgtttcgctt attttactga tcctcgcctc accttttatg
     1441 ctaatcacag cgattttggt aaaaacaact tcacctggac caatcatcta taaacaagta
     1501 cgtattacga aaaatcaaaa ggaattttca atctataaat tccgttccat gagtgcgaca
     1561 gctgaagcaa aatcaggacc agtcttggct aaaagtaacg atgcacgtgt gacaccagta
     1621 ggtaaattta tccgtgctgt acgttttgac gaattacctc aaatctttaa tgtattgaaa
     1681 ggcgatatgt cgattgtcgg tccaaggcca gaaagaccat ttttcgttga tcagttcaat
     1741 gaagaaaatc cttattatta cttaagacac aatgtacgtg cgggaattac cggatatgca
     1801 caagtatacg gaaaatatgt atcagattat aacagtaaac tacgttttga tttgttgtat
     1861 atcaaaaaat attcattact attagatttg aagattttac ttcaaacaat caaaatctta
     1921 tttgataaga tgtcatctca aggactagat gaagatgaaa atattagttc attagcagaa
     1981 atcgactttt cacaagagaa aatttatcgc tagattggaa aaattttgag caaagaggta
     2041 ggagagaaca ttggaaaata aaaatgtaag tatgctgctt tctttatatg ataaagaaaa
     2101 gccagagtat ttaaaggaag cattaactag tatttttgag caaacagtaa tgccaaaaga
     2161 aattgtatta gtttatgacg gccctataaa tgaaaaactt caagtagttg tcacttattt
     2221 ccaacaaaaa gatcctcact tgtttacagt tgtaaaatta gaaaaaaatc aaggattggg
     2281 gattgcttta gcaattggat tagaacatac gaaaaatcgc ttagtagcca gaatggatac
     2341 agatgatata atggaacccc aacgattaga aaaacaatta gagatcttcc aaaaagatcc
     2401 agaaatagcg attgttggga gtaatattga ggaatttact ggcacgctta gcaatgttat
     2461 cggaaaaaga attgtacctg aatataacaa tgcaattcgt gtattttcta aaagaagaaa
     2521 tccctttaat catatgaccg ttatgtttga taaaaaagct attttggaag tgggaaatta
     2581 ccaaccatta agtgggtttg aagattatta tctttgggca agattattaa aggcaggtta
     2641 taaaggatat aatattcaag aaacattagt ctatgcgcga actggcaaag atatgtatgc
     2701 aagaagaggc ggaatgaaat atcttggttc tggtcttatg ggtcgttatc gtatctggaa
     2761 agacggttta gggtcagtaa aagattttat aattgtttct tgcggacata tttttattag
     2821 cttactacca aatcaattaa gaggaaaatt ttatcaaacg caattaagaa aataggaggt
     2881 ttttctttgt atagcactga tccaaaaatg aaaaaaatac aacagattac gttgaaaatg
     2941 tctcaagaga ttgttgattt ttgtctaaaa aatcaacttc tttgttattt ctgcggtggt
     3001 ggtgccatcg gtactgtgag agaacaaggg tttattccat gggatgatga tttagatttc
     3061 tttatgccta gagaggatta tgaaaaattt tatcaactat gggttaattc agaaaaaagt
     3121 aaaaaatatc ccatacagaa ggcttctaaa aattataatg atcataattc ttttacaact
     3181 atccgcgatg cagatactac atttattaaa acttatcaaa gagatttaga tatagtccat
     3241 ggaattacta ttgatatttt cccgttagat gttgcccctg tttcgaaatt tagccgtaaa
     3301 attcaaaaac tatgggcttt agtatatgct ttattttgtt ctcaagtcgt ccctgaaaaa
     3361 catggtggaa taatggctac aggtagtaag atactcttat ctattttccg ctcacctcgt
     3421 acgagatata agatatggtc ttttgctgaa aaacaaatga caaaatataa taatgaacca
     3481 acgggctatg tgactgaact ttgtgtcgga cctaaataca tgggaaatgt ttatcctgca
     3541 aaagactttg aatctgctgt gtgggtgcca ttcgaagata cagagatgcc agtaccagtt
     3601 ggttatgata attatttaag tcaagttttt ggtgactata tgcagcttcc gcctgaaaaa
     3661 gatcaggttt ctcatcatga agcagtttat attgatccag aacatccata cacaatgtac
     3721 aaaggaaaat attatttaac taaaggagca gaaaaaaaat gattacagct attattattg
     3781 ctggtggtgt gggtaaacga atgggacaag atatcccaaa acagttcatt acaattgaag
     3841 gaaaacctat tattatctat actttagaat catttcaaca ccatcctcaa gttgaccgga
     3901 ttttagtcgt ttgcaaatct ggttgggaag aaacgatgtg ggcgtatatc aaagaattca
     3961 atattagcaa agtcaaatgg gtcattacag gtggatcaaa gggacaagag tcgatcaata
     4021 atggtgttca gtttttgaaa gactattctg aagatgatga tacgatcatc atccatgatg
     4081 gaatccgtcc actagtagac gaactggtat tgtcagatgt aatcgttaaa tgtaaagaat
     4141 ttggtaacgg tgtaacctct ctaccttaca acgaacaaat ttttatcaag aaaacagaag
     4201 aaacgacgga acagtatgtc gatcgtaaca cattgaggcg tgtctcaact ccacaagctt
     4261 atcaatacgg aaaactacgg gcagcatatg accgggcagt gaaagaagac atcggtatga
     4321 ctgattcttc ttatacgaat accatgatgg tagatttggg agaaacttta tattttgctg
     4381 ctggttctga taaaaatatc aaattgacaa caacagatga tctagaattg ttcaaagcat
     4441 atttaaaaat gaaagactag gtgaaagatg atgacgttga ttaaacaacc aatgtaccaa
     4501 gaagatttgg caaaagtaat caatcataca ccaaaaataa aacagctaca tggaaaatcc
     4561 gttctgatga ttggtgcttc tggaatgatc ggctcttttt tgattgatac gttgatggct
     4621 gctaatgaac aactaggcat caaaatcaaa gtttatgcaa tgggcagaaa ccgtcagaag
     4681 cttgaaaaaa ggtttgccag ttatcttgaa ttagatactt tcgaaattgt tgaaggtgac
     4741 gtcacagaac ccttaccaaa agaaattcaa gctgactatt tgatacacgg tgcaagtaac
     4801 acacatccta aggcttacgc aactgatcca atcggtacga tcatgactaa tcttgcaggt
     4861 acagagcaag tattgaaaca tgcagtagca acccaaccgg ttagagtatt atttttatca
     4921 acagtagaaa tctatggcga aaatcgaggc gatatagaaa aattcacaga agattactgt
     4981 ggatacatcg attgtaatac actgagagca ggctaccctg aaggaaaaag agtcagtgaa
     5041 tcattatgcc aagcctacat cgcaaaacac gacctcgaca tcgtgattcc aaggctttgc
     5101 cgtacgtttg gtccgacgat gcttttatct gatacgaaag catcctcaca atttatttta
     5161 aatgcagtcg aacaaaaaga tattgtttta aaaagtgaag gaaaccaata tttttcgtat
     5221 gtatatgttg gagatgctgt ttctgctatt cttcatttac tgcttgaagg agaaaaagga
     5281 gaggcgtaca acgtttcttc agaaaacttt gatttacgac taaaagaatt agcccaacag
     5341 ctagctgata tcagcggcaa acaagtaatt tttgaattgc cggatgagat tgaacaaaaa
     5401 ggtttctcaa aagctagcac agcagtactt gaaaataaaa aaattaaaga gacaggttgg
     5461 aaaccaattt ttcctttgaa cgaatcattg gttcatacag tagaattagt taaaggtgag
     5521 aaatataatg tgtgagatta gtattattgt tcctgtgtat aaagttgaaa aatatttaag
     5581 aaaatgtgta gattcgattt tagcccaaac atttactgat tttgaagtta tattagtaga
     5641 tgatgg
//
LOCUS       DAZKLD010000159         5395 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.161, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000159 DAZKLD010000000
VERSION     DAZKLD010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5395
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.161"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1342
                     /gene="gmhB"
                     /locus_tag="ACG8KR_002602"
     CDS             <1..1342
                     /gene="gmhB"
                     /locus_tag="ACG8KR_002602"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HFQ0201915.1"
                     /translation="HIMKTVIMAGGRGSRISELFPDIPKPMIPIDGIPVLERELCSLR
                     DQGFNDIILTVSYLHEKIEEHFGDGSKLGIKIKYFIEKTPLGNAGALYFLLDELGDEP
                     FLLLNADAVFDVDFRQMIRFHVENNADVTLFTHPNSHPYDSGLIISDDNKKVLQWLAK
                     EDQRPEYYKNRVNAGLHVINPKVLRENPLMEIVNKSIVEGGGLPYKIDLDRMILKPLA
                     NTGRMYCYDSPEYVKDMGTPDRYRAVIKDFQKGIIVGKNLSNPQKAIFLDRDGTINKY
                     VGFLRNAEEFELIDGVSEAIKIINSLGFLCIVITNQPVIARGEVTVDELNSIHNKLET
                     MLGHAGGFLNGIYYCPHHPHKGYAGEVIELKIDCNCRKPKPGLLLEASKDFNINLSQS
                     FMVGDSESDVIAGKNAGCKTVLITEDITLPANYGQDYSVCSLLEFVKNHLCTMQ"
     gene            1377..3014
                     /locus_tag="ACG8KR_002603"
     CDS             1377..3014
                     /locus_tag="ACG8KR_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular biosynthesis protein"
                     /protein_id="HFQ0201916.1"
                     /translation="MKVLIPAAKVVEKELQSIGMLPAIIYPINKKIVFDYLYNQYNAI
                     CDSLDIICYEKADKVHRRLKNYHDSRIHICDLPELGDLGQTVYFGLSDEDEPVLINFA
                     DTIVMESMSDIEMDTFYYSEDFVSSTWTYFKINEGIITDIHDKEEAYVSDKREKLFIG
                     VFMFSSSKLLKSCLREAVESRKQGGINSFYRALQLYSERCPLKAVRTEKWFDIGHIDK
                     YYNTKLEVKAREFNHISIDKDRGILKKTSDDKDKFIGEIKWYLKLPADIEYVRPRIFD
                     YSTSYVAPYVDMEYYAYHTVHELYLYGDLKYNQWHDLFSRIKFICSDFKRYTLKDEKI
                     RESLEDMYLRKTMQRFQKLSEDKRFSAFFEKQIQINGRKFISLTGVIELLKNLIPSML
                     YDVNEFCIIHGDLCFANMMVDSNCSFVKVIDPRGKFGKYDIYGDSRYELAKLFHSLDG
                     KYDYIIKDLFTVEYDTECAVIDYTINTRVRDYDLFELFKSVFSDEIKDDFDKIVLIEA
                     LLFLSMIPLHGESFDHQIVMLATGMELLARVSNKVIL"
     gene            3116..3511
                     /locus_tag="ACG8KR_002604"
     CDS             3116..3511
                     /locus_tag="ACG8KR_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular biosynthesis protein"
                     /protein_id="HFQ0201917.1"
                     /translation="MYSEYSFVFDIDGTLCPIKKKDERYEDLVPYENMVEKLRFYKEN
                     GAKITLFTSRNVNSYKGNLGMINANTAKVLLAWLDKWDIPYDEILYGKPWPGHKGFYV
                     DDRTVRPDEFLRCSVDELNEICNSSKKRF"
     gene            3529..4260
                     /locus_tag="ACG8KR_002605"
     CDS             3529..4260
                     /locus_tag="ACG8KR_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFQ0201918.1"
                     /translation="MEKKMTVIITMAGLGSRFRKIGYAVPKYMINAKGRTLFDWAMDS
                     LVGYNKNVDKYIFVVRQEDNASAFIKEHCAYFGIENVEVIEIDYMTDGQATTCMLAIP
                     YCNQDNEIMVYNIDTYVESNEMKYEDICGDGHIPCFHADGDHWSFAKLNDRNEVIEVR
                     EKVRISDNCTLGAYYFSSAKLYKALYEEYYADSSNMEKNEKYIAPLYNFMIKKGLKVT
                     ISIVDAKKVHVLGTPEELNVFLNQE"
     gene            4284..>5395
                     /locus_tag="ACG8KR_002606"
     CDS             4284..>5395
                     /locus_tag="ACG8KR_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HFQ0201919.1"
                     /translation="MTSNNLIVKTLSRYKNTPLPVKAALWFMVCGFLQRGVSMITTPI
                     FTRVLSTEQYGLYSTYLSWESLLLMITSLSLFKAMMNLYVKYDNQEHILSTVSGLTLV
                     LTIFWFVIAIFIKDILAEILGLTPVLVVCMFSYFIFQAEIQCWSLHKRYIYDYKTLLV
                     ATLAVTVGSSVVGCLAVLFISPTAEARALMNVLVTGVVGVAVYISVFRRCKDFKDNEV
                     WNFAVVFCVSLMPHYLSEFILNGSDKIMINYLCGSSDVAIYSVAYSIGALINLVTNSV
                     NSTFAPYQYQKIKSGEYDLLSKRANEVLGLVAAMLFALMLFSKEIVLIFGGQKYIDSV
                     NLIVPICIGIYFNYMFQLFARVQEYFEKKLTVVIPSV"
BASE COUNT         1779 a          692 c         1125 g         1799 t
ORIGIN      
        1 gcatattatg aaaacagtaa ttatggcggg cggacgagga agccgaattt cggaattgtt
       61 tccggatatt cctaagccaa tgattcctat agatggcatt ccagttttag aacgagaatt
      121 gtgttcgctt agagatcaag gctttaatga tattattttg actgtttcct atttgcatga
      181 aaaaatagag gagcatttcg gagatggttc aaaattagga attaaaatca aatattttat
      241 tgaaaaaact ccacttggca atgcaggtgc attgtatttt ttgttggatg aactgggtga
      301 tgaaccgttt ttacttttga atgctgatgc agtatttgat gttgatttca ggcagatgat
      361 tagatttcat gttgagaata acgcagatgt tactttgttt acgcatccta actcacatcc
      421 gtatgatagt ggattaatta tatccgatga caataagaaa gtgcttcaat ggttagcgaa
      481 ggaagatcaa agacctgaat attataaaaa ccgagtaaat gcaggtcttc atgttataaa
      541 tccgaaggtg ttgagagaaa atccattaat ggaaattgta aataaatcaa tagttgaagg
      601 tggggggcta ccatacaaaa ttgatttaga tagaatgatt ctgaaaccat tggctaacac
      661 agggcgtatg tattgctacg attctccaga gtatgttaaa gatatgggaa caccggaccg
      721 atatagagct gtaattaagg actttcaaaa gggaattatt gttggtaaaa atctatccaa
      781 tccacaaaaa gcaatctttt tagatagaga tggaacgatt aataaatacg ttggtttttt
      841 gagaaatgca gaagaatttg agttaattga tggagtatcg gaggcaatta aaattattaa
      901 ttctctaggt tttctgtgta ttgttataac aaatcagcct gtaattgcaa gaggtgaagt
      961 tactgttgat gagcttaata gtattcacaa taagcttgaa acaatgctag ggcatgctgg
     1021 tggattttta aacggtattt attactgtcc gcaccatccc cataaaggat atgcaggaga
     1081 ggttatagaa ttaaaaattg attgcaattg tcgcaagcca aaacctggac tgttattaga
     1141 agcatcaaag gattttaaca taaacctaag tcaatcattt atggttggag atagtgaaag
     1201 tgatgtaatt gctggaaaaa atgctggttg taagacagta ctgattacag aagacatcac
     1261 cttgcctgct aattatggcc aagactatag tgtctgttca ttgttagaat ttgttaaaaa
     1321 tcatttatgc actatgcaat aattggttta gtgaaacttg taaaaggagt aattagatga
     1381 aggtattaat tccagcagca aaagttgttg aaaaagaatt gcagagtata ggaatgttac
     1441 cagcaattat ctatccaatt aataaaaaaa ttgtctttga ttatttgtat aatcagtata
     1501 atgcaatctg cgattcattg gatattatct gttatgaaaa agcggataag gttcatagaa
     1561 gactgaaaaa ctatcatgat tccagaattc atatatgtga ccttcctgaa ttaggggatc
     1621 tagggcaaac cgtgtatttt gggttatcag atgaagatga gcctgttcta attaacttcg
     1681 cagatacaat tgttatggag agtatgtctg atatagaaat ggatacattc tattattctg
     1741 aagattttgt gtcgagtact tggacatact ttaaaattaa tgaaggaata attactgata
     1801 tccacgataa agaggaagct tacgtatccg ataaacgtga aaaattattt attggcgtat
     1861 ttatgttttc ttcgtcaaaa cttctgaaga gttgcttaag agaggcagtt gagagtcgga
     1921 aacagggggg aataaactca ttttatcggg ctttacagct atacagcgaa cgttgtccgc
     1981 taaaggccgt aagaacagaa aagtggtttg atattggaca catcgataaa tattacaata
     2041 caaagttgga agttaaagca cgagagttta atcatatctc tatagataaa gatcgaggga
     2101 tattgaagaa aactagcgat gacaaggata aatttattgg agaaataaag tggtatttaa
     2161 aacttcctgc tgatattgaa tatgtaagac ctagaatttt tgattattct acatcttacg
     2221 tagctcccta tgttgatatg gaatattatg catatcatac ggttcatgag ttatacttat
     2281 atggtgattt aaaatataac cagtggcatg atctgtttag ccgtataaag tttatctgta
     2341 gcgattttaa gcgatacacc ttaaaagatg aaaagattag agagtcattg gaagatatgt
     2401 atttgcgaaa aaccatgcag agatttcaaa aattgtctga agataaaaga ttttcagctt
     2461 tttttgagaa acaaattcaa attaatggtc gtaagtttat ttctttaacc ggtgtaattg
     2521 aacttctaaa gaatcttatc ccaagtatgc tctatgatgt aaatgaattt tgtattatac
     2581 atggagattt gtgttttgct aatatgatgg tagattccaa ttgttccttt gttaaggtta
     2641 ttgatcctag gggaaaattt ggaaaatatg atatttatgg ggacagcaga tatgaattag
     2701 caaaactatt tcatagttta gatggtaaat atgattatat catcaaggat ctatttactg
     2761 tagaatatga taccgagtgt gcagtgatag attatacaat taatacgcgt gtaagagatt
     2821 acgatttatt tgaattgttt aagtcggtct tttcggacga gattaaggac gattttgata
     2881 agattgtatt gatagaagcg ttgctatttc ttagtatgat tccattacac ggtgaaagtt
     2941 ttgatcacca aatagtgatg cttgctacag gtatggaact tcttgcaaga gtaagtaata
     3001 aagttatact ctaatataaa ttgtgcctta atgaataacg gttacaatac tgttttagca
     3061 atgtaggcat taaggcacga ttatattaat taatacaata gaagggaaga gaaaaatgta
     3121 tagtgaatat tcttttgtgt ttgatatcga tggcactttg tgtccgataa agaagaagga
     3181 tgagcgatat gaggatttgg tgccgtatga gaatatggtt gaaaaactga gattctataa
     3241 ggagaatggt gctaaaatta cattgttcac ctcacgcaat gttaattcct ataaaggaaa
     3301 tttgggaatg ataaatgcta atactgcaaa agtgctcttg gcgtggttgg ataaatggga
     3361 cattccgtat gatgaaatcc tgtatggtaa gccttggccg gggcataaag gcttctatgt
     3421 agatgatcga acggttagac ctgatgagtt tttgagatgc tctgtagatg aattaaatga
     3481 aatctgcaac agttcaaaaa aaagattcta aataaggagg ttagttctat ggaaaaaaag
     3541 atgacagtta tcattacgat ggcaggtttg ggaagtcgtt ttcgaaaaat tggttatgcg
     3601 gttcctaaat atatgattaa tgccaaagga agaactttat ttgattgggc catggatagt
     3661 ttggttggat acaataaaaa tgttgataag tatatttttg ttgttcgtca ggaagacaac
     3721 gcttcagctt ttataaagga gcattgtgct tactttggta ttgaaaacgt agaagtgatt
     3781 gaaatagact atatgacaga tggtcaagca actacatgta tgttggctat tccttattgc
     3841 aatcaagaca atgagattat ggtttacaat attgatacat acgtggaatc aaatgagatg
     3901 aaatacgaag atatttgtgg agatggacat attccatgct ttcatgctga tggcgatcat
     3961 tggagttttg caaagcttaa cgatcgaaat gaagtaatag aagtccgcga aaaagttcgc
     4021 atttctgata attgtactct tggtgcgtat tatttctcat cggcaaagct gtataaagct
     4081 ttatatgaag agtattatgc agactcctct aatatggaga agaatgaaaa atatatagca
     4141 ccattatata actttatgat aaaaaaaggg ttaaaggtga ctattagcat agtagatgcg
     4201 aaaaaggttc atgtgcttgg tactccagaa gagctcaatg tgtttttgaa tcaagaataa
     4261 atagtgttta ggagaaaata gcagtgacca gtaataatct aattgtaaaa acattaagca
     4321 gatataagaa tactccactt cctgttaagg ctgcattatg gtttatggta tgtggttttt
     4381 tacagagagg ggtttcaatg attacaaccc caatttttac tagagttttg tctacagaac
     4441 aatacggttt atacagcact tatctatcgt gggaatcctt attgctcatg attacatcgc
     4501 ttagtttgtt caaagccatg atgaatcttt atgtaaaata tgataatcaa gaacatattc
     4561 tttcgacagt atccggattg actttagtac tgactatttt ttggtttgtt attgctattt
     4621 ttattaagga tatattggca gaaattttag gattgacacc agttctagtt gtctgtatgt
     4681 tctcatattt tatatttcag gcagaaatac aatgttggtc tttacacaaa cgatatatat
     4741 atgattataa gactttatta gtagctaccc ttgcagttac tgttggctca tctgtagttg
     4801 gctgtttagc ggttctgttt atatctccta ccgctgaggc gagagcctta atgaatgtcc
     4861 tagtgactgg tgttgttggc gtggctgtgt atatatcggt atttagaaga tgtaaggatt
     4921 ttaaggataa tgaagtgtgg aatttcgcag tagtattttg tgtatcgttg atgccacact
     4981 atttatctga atttattctg aatggttctg ataaaattat gataaattac ttatgtggtt
     5041 cgtcagatgt tgctatttac agcgtggcat attctattgg tgctctaatt aaccttgtta
     5101 caaattctgt caattccact tttgcacctt atcagtatca aaaaatcaag tcaggagaat
     5161 atgatttact atcaaaaaga gctaatgaag ttcttggttt ggtagcggct atgctttttg
     5221 cattaatgct atttagtaaa gaaattgtgt tgatatttgg tgggcagaaa tatatcgaca
     5281 gtgttaattt aattgtacct atatgtatcg gtatttattt caactatatg tttcagctgt
     5341 ttgctagagt acaggaatac tttgaaaaga aactcactgt cgttattcca tcggt
//
LOCUS       DAZKLD010000160         5323 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.162, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000160 DAZKLD010000000
VERSION     DAZKLD010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5323
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.162"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(46..321)
                     /gene="prgO"
                     /locus_tag="ACG8KR_002607"
     CDS             complement(46..321)
                     /gene="prgO"
                     /locus_tag="ACG8KR_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA segregation protein PrgO"
                     /protein_id="HFQ0201920.1"
                     /translation="MALLPTNKDKKKIGAEDISIKDDINVELGERKYTAKERKPVQVD
                     PPVLKTIRDLSYAKDIPMYEIVKLAMDAYVDTLNDEERIIYNRRSKD"
     gene            complement(293..1246)
                     /locus_tag="ACG8KR_002608"
     CDS             complement(293..1246)
                     /locus_tag="ACG8KR_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFQ0201921.1"
                     /translation="MENLRTAFKTSDKLGKVVMLTGRKGGIGKTTDNDLLAIVSSQLF
                     EKDVLLIDYDQQRNTTSNIGSTYQITSFDRSMSAAIKKGDWVSGITQVSPHLYIMAGS
                     PGSEELNEYLSEKYPDRRKRSLAFIKPLEELRKNFDYIFIDCPPSTDNVVRAFLTAAD
                     YIIAMQELKRYAMEGTEDFINKVLVPIVTNFEESHLQIIGILPVLFSVRRSSQHANYQ
                     KTIDKYGESNIFKSIVKGSDRLEMYGENGIQLNDYVDRRWWAIFADIFTELEERIEYY
                     EQHGDIEGFDYTPKYADSMANHILPLGKEIKLNGIITNEQR"
     gene            1858..3351
                     /locus_tag="ACG8KR_002609"
     CDS             1858..3351
                     /locus_tag="ACG8KR_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase C-terminal domain-containing protein"
                     /protein_id="HFQ0201922.1"
                     /translation="MLSEIYSAILKNGLRTYKYRNSTMKPAGHIDSSRSGAIFAYRSK
                     QLMNVGRGMVITSEEAILENEKKLTHWTPNTYRFGTYADDYRTVVKGHSEKNLSQINT
                     FVVDIDSKENHQGEIILACLDQVGYMPTLILESDHGYQVYFVLKTPGYVTKKSNFKVI
                     DVAKKISKTIRLQLAEKLSGVDLGCNHFGIARFPQENNIVFKEMNYQYSFADWIQWSM
                     KLESDQKTKEDRERKLIVFPEKKEIRQVDEPWFDMLLHKANIVGGEGRLGRNNVIFTL
                     SLAYYSSGYALDTCEYNMFTFNERLQEPLSEAELVKIVRSAYSGNYQAASREFIIELC
                     QQWVSPTLKNNDLFIQRRGWWKFKKPRDQRKYSHKNEWKEDLLAYLSEKSLASEPYLN
                     LSKKELASQLTMPKRTLDKILNELREENKVFYKVTRGRNGGMIIASVKTLFARVIQLS
                     KEKRLDYSTRITEVFGLNESLVEETFRQLSTPLNPLKEVNLFEVDTG"
     gene            3486..3782
                     /locus_tag="ACG8KR_002610"
     CDS             3486..3782
                     /locus_tag="ACG8KR_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HFQ0201923.1"
                     /translation="MSLKNYVYSHPVNVFIIGRLGLSVEVFCELYGFKQGTLSSWVTR
                     EKTVASLPIEFLHGLSLASGETMDQVYDCLCVLEQEYIEYKIANELRKRKKYIQ"
     gene            3883..4734
                     /locus_tag="ACG8KR_002611"
     CDS             3883..4734
                     /locus_tag="ACG8KR_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0201924.1"
                     /translation="MKSDDWIEHTWLPLKRNSKRKKFWVLQLWPLGKLCSYGARLILD
                     LTVYSIGVFGTAYYLINDPNRIAASDIFTVLMTLIFVMLLSIFTQLVMQFTRQKLTAG
                     KLFKSLFDLIVVNAILATLIKQIEQSGRTTKGVKDFLLTFIHFVNGHMYLFEVGIILF
                     FVGYVSTKLIKRSIEKEIEIDLIPTLLGYEYNVDFGRYDELINTDIVHTSIHESTVKG
                     IRSILKNGQEMKAIDRRKEIYNFYEVEASLRYGIGKLWLDGSNIRQNKPYIFWNSSDL
                     ELPMKDK"
     gene            4755..4967
                     /locus_tag="ACG8KR_002612"
     CDS             4755..4967
                     /locus_tag="ACG8KR_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0201925.1"
                     /translation="MIPLYRIVLIVGMELKTLKKEQLFKVKERHNQLLCSKTGTMNIE
                     YRTEYLELTNQTDFLLIRKQQEQEKN"
BASE COUNT         1819 a          826 c         1000 g         1678 t
ORIGIN      
        1 aagtatacta aatatattta ctatatctag tatacttact atattttaat cttttgatcg
       61 tctattatat ataatccgtt cctcgtcgtt taaagtatca acataggcgt ccattgcaag
      121 tttgactatt tcatacatag gaatatcttt tgcataacta agatcccgaa tggtttttag
      181 tacgggagga tctacttgaa ctggttttct ttctttcgca gtgtactttc gttcccctaa
      241 ctcgacatta atatcatctt taattgaaat atcttcagca ccaattttct tcttatcttt
      301 gttcgttggt aataatgcca tttagtttaa tctcctttcc aagaggtagg atatgattgg
      361 ccattgagtc agcatattta ggtgtataat caaatccttc aatatcacca tgttgttcat
      421 agtattcaat tctttcttct aattctgtaa agatgtctgc aaaaatagcc caccatcttc
      481 gatctacata atcatttaat tggataccat tctcaccata catttctaac cgatccgagc
      541 ctttaacaat gcttttaaaa atgtttgatt caccatactt atcaattgtt ttttgatagt
      601 ttgcgtgttg agaggaacgt cgcactgaga aaagaacagg caaaattcca atgatttgaa
      661 gatgagactc ttcaaaattc gtgacaatcg gaactaaaac tttattaata aaatcttctg
      721 ttccttccat tgcgtaccgt tttaattctt gcattgcaat aatataatct gcagcagtta
      781 agaatgcacg aaccacatta tctgttgatg gtgggcagtc gataaagata taatcgaaat
      841 tttttctcaa ttcttctaaa ggtttaatga aggccaatga tctcttacga cgatcaggat
      901 atttttcgga caagtattca ttaagttctt cagatccagg agagccagcc ataatatata
      961 aatgtggact tacttgagta atacctgaaa cccagtcccc ttttttgatt gctgcagaca
     1021 tggatcgatc aaagctagta atttgatacg ttgatccaat gttggaagta gtatttcttt
     1081 gttggtcata atcaattagc aacacgtctt tttcaaaaag ttgactagaa acaatcgcta
     1141 ataaatcatt gtctgtagtc ttaccaatgc cgccttttct acctgtaagc attacaactt
     1201 ttcctagttt atctgatgtt ttaaatgcag tccgtaagtt ttccatagtt ccctccaatt
     1261 tcctatacta aatatattta gtatacttca tatacttaat atattcgata tatctaatat
     1321 actccaaatt gataagaatt cagactttat aaggaatata gtggatatac taaatatatt
     1381 tgctatattc agtatatcag atatattcaa aatgtcaaaa tatatttgtg cgttttgcgt
     1441 tgacaattta gagtgaaaag aggtaaactt aattctatag aaaccacaga taattcacta
     1501 tttacaaagt agcaaaagca gtggtactct ttaaacagaa aaattacata aaaaaaagag
     1561 acggaaataa ttgattttag cagaatcaat tatttctgaa tcacatggtt aggacatgtg
     1621 ttcacgctcg tctcaagaat atgtaagtag ctaccaacta cttatattaa aagttgatcg
     1681 gatacctttt ccgattgcct ttattctacc aaaaaaaggc gagcaactca acagaagaac
     1741 gtttatttag tgtacttaaa ttaaatattc ttaaacactg aagagagaac acatactatg
     1801 gctgagatta aagctaaact gtgttctctt tttgtttgag aaaggacgtt acaatcgatg
     1861 ttgagcgaaa tttattcagc tattcttaaa aacggcttac gtacatacaa atatcgaaac
     1921 agcaccatga aacctgcagg tcatattgat tccagtagga gtggtgctat ctttgcgtac
     1981 cgtagcaagc aattaatgaa tgttgggcgt ggtatggtca ttacttcgga agaagctatt
     2041 ttagagaacg aaaagaaact aacgcattgg acacctaata cctatcgctt tggtacttat
     2101 gcggatgatt atcgaacagt ggttaaaggt cattctgaga aaaacttaag tcaaatcaac
     2161 acatttgtag ttgatatcga ttcaaaagag aaccatcaag gcgaaattat tttagcctgt
     2221 ttagatcaag tgggctacat gccaacgcta attttagaat cagatcatgg ttatcaagtt
     2281 tattttgtac taaagacacc aggctatgtc acaaagaaaa gcaatttcaa ggtgatcgat
     2341 gtagcaaaaa aaatttcaaa aacaattcgt ctacagttgg ctgaaaaatt gtccggagtg
     2401 gatctaggat gtaaccactt cggaattgct cgattcccac aagaaaataa tattgttttt
     2461 aaagaaatga actatcagta ttcttttgct gactggattc aatggtcgat gaagcttgaa
     2521 tcagatcaaa aaacaaaaga agatcgagaa cgaaaactaa ttgtctttcc tgagaaaaaa
     2581 gaaattcgtc aagtagatga accgtggttc gatatgttac ttcataaagc aaacatcgtc
     2641 ggcggagaag gacgtcttgg tcgaaataat gtgattttca cgctttcttt agcttattac
     2701 tcttcaggtt atgcattgga tacttgcgaa tacaatatgt tcacattcaa cgaacggtta
     2761 caggaaccgc tatctgaagc agaattagta aagattgtac gtagtgctta ttctggtaat
     2821 tatcaagcag caagcagaga attcattatt gaattgtgcc agcaatgggt ttcaccaacc
     2881 ttaaaaaata atgatctgtt cattcaacga cgtggctggt ggaaatttaa aaagccacga
     2941 gatcaaagaa aatatagtca taaaaatgaa tggaaagaag atctattggc ttatctaagc
     3001 gaaaaaagtc ttgctagtga gccttatttg aacctgtcaa aaaaagaatt ggcttcacaa
     3061 ctaacgatgc ccaagcgaac acttgataag atcttaaatg agttacgaga ggagaataaa
     3121 gtcttctaca aggtaacacg aggaagaaat ggcggaatga tcattgcttc agtcaaaacg
     3181 ttgttcgcga gggttattca actaagtaaa gaaaagagac tagattattc aacaaggatc
     3241 acagaggtat ttggactcaa cgagagctta gtagaagaga catttagaca attatcaacc
     3301 cccttgaacc ctcttaaaga agtgaatctt tttgaagtag atacaggttg aaaaataaaa
     3361 ggtgcaaatt gccctataca atatatctat gatacttctg taggtgttgc tccctttgcc
     3421 tagctacgtg taggtggtat acttataact gcgtatgcag tcttatgtat catggaggaa
     3481 gttgtatgtc gttaaaaaat tatgtctatt cacatcctgt taatgtgttt atcattggtc
     3541 gtttgggatt atctgtggaa gtgttctgtg agctttatgg ttttaagcag gggactttgt
     3601 cttcttgggt taccagggaa aaaacagttg cttctttgcc gattgagttt cttcatgggt
     3661 tgtccttagc ttctggggag actatggatc aggtatatga ttgtttgtgt gtgttagagc
     3721 aggagtatat tgagtataaa atagctaacg agttaagaaa aaggaagaag tatatccagt
     3781 agtagttttt atttacttaa aaaattggtt cattgagcaa cctatcgctg ctaaagtagc
     3841 aaaggaaaac gttaaaggaa agaatttagg gaagaaatcg aaatgaaatc ggacgactgg
     3901 attgaacaca cctggcttcc tctgaaaaga aatagcaaac gaaagaaatt ttgggtattg
     3961 caattatggc cattaggaaa actttgtagt tacggggcac gacttattct tgatttaacc
     4021 gtttactcga ttggagtatt tggaacggct tattacttga taaatgatcc caataggatt
     4081 gcagcatcag acatttttac agtgctaatg acgttgattt ttgtcatgct attgtctatt
     4141 tttactcaat tagtcatgca atttaccagg caaaaattaa cagcaggaaa acttttcaaa
     4201 agcctattcg atttaattgt tgtaaatgca attctagcga ctttaataaa acaaattgaa
     4261 caatctggaa gaacaacaaa aggagtgaag gattttttat tgacgtttat tcactttgta
     4321 aatggacaca tgtatttgtt tgaagtgggt attattctct tttttgtcgg gtatgtatcc
     4381 acgaaactta ttaaacgttc aattgaaaaa gaaattgaaa ttgacctgat tccgacactt
     4441 cttggttatg agtacaatgt tgattttgga agatatgacg aattgatcaa tacggatatc
     4501 gttcacacga gtatccatga atctactgtt aagggcatca gatctatttt aaaaaatggc
     4561 caggaaatga aagcaataga tcgaagaaaa gagatctaca atttttatga agtcgaagca
     4621 agcctacgat atggcatcgg aaaattgtgg ttagatggaa gtaatattcg tcaaaacaaa
     4681 ccatacattt tttggaacag tagtgattta gaacttccaa tgaaagataa ataattatgg
     4741 agaatcgttt gaaaatgatt cctctataca ggattgtgct tatagttggc atggagttaa
     4801 aaaccttaaa gaaggagcag ttattcaaag tgaaagaacg tcataatcag ttactatgta
     4861 gtaaaacggg tacgatgaac attgaatacc gaacagaata tttagaatta acgaatcaaa
     4921 ctgatttttt gttgattcga aagcaacaag agcaagaaaa aaattaatgg ctacttatag
     4981 ccaattgatt gatgaatggg aacaattcaa ggaatctaaa attccagaac gtctctaaaa
     5041 gcctttattt gtaaagagca taaggaaacg tgggaacact ttttaaaaaa agtgttccaa
     5101 taggctgtgc ctattacgtt tctcaaaaat aaatgtggga acaaaatatt taaaaatagg
     5161 atagtgaaag gatagtgaag gatagtaaaa ggagggtagt aggatagcaa aaaggatagg
     5221 ctccttttcg ctatcctttt taaattggag gaattaatat ggaagtaaga ataagaaatg
     5281 tggatgagaa aatcgtaaga gaaattgatc gaagatgtga cga
//
LOCUS       DAZKLD010000161         5315 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.163, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000161 DAZKLD010000000
VERSION     DAZKLD010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5315)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5315)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5315
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.163"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(45..1022)
                     /locus_tag="ACG8KR_002613"
     CDS             complement(45..1022)
                     /locus_tag="ACG8KR_002613"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HFQ0201926.1"
                     /translation="MAQKTMIQAITDALALELENDENVVVFGEDVGKNGGVFRATEGL
                     QEKFGEDRVFDTPLAESGIAGLSFGLALEGFRPVPEIQFFGFIFEAMDEVVAQMARTR
                     YRMSGTRNLPITIRSPFGGGVHTPELHSDNLEGLIAQSPGIRVVIPSNPYDAKGLLIS
                     SIRSNDPVVFLEHMKLYRSFREEVPDEAYEVPLDKAAVTREGTDVSIITYGAMVREAI
                     KAADNLAKENISVEIIDLRTVAPLDVETIIQSVEKTGRVVVVQEAQRQAGVAAQVVSE
                     ISERAILSLEAPIGRVSAPDTVFPFGQAENVWLPNAKDIEDKVKEIAEF"
     gene            complement(1025..2134)
                     /gene="pdhA"
                     /locus_tag="ACG8KR_002614"
     CDS             complement(1025..2134)
                     /gene="pdhA"
                     /locus_tag="ACG8KR_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HFQ0201927.1"
                     /translation="MANKKAPIDFQALLDEVNADFPVYQALDQDGNVVNKDLVPDLSD
                     DELVELMTHMVWSRVLDQRSTALNRQGRLGFFAPTAGQEASQLASAYAFDKEDVLLPG
                     YRDVPQLVKHGLPLSQAFLWSRGHAAGNSYPEELKALPPQIIIGAQYVQAAGVALGLK
                     KRNKKNVVFTYTGDGGSSQGDFYEAINFAGAYHANAVFYIQNNGYAISTPREKQTAAK
                     TLAQKAVAAGIPGIQVDGMDPLAVYTVSKMAREWAISGNGPVLIETLTYRYGPHTLSG
                     DDPTRYRTKDTDDDWQKKDPLIRFRKYLTEKGLWSEEKEEQVIEATKEEIKAAIAEAD
                     KVPKQKVSDFLKNMFEVSPQSIKEQIAIYEAKESK"
     gene            2296..2553
                     /locus_tag="ACG8KR_002615"
     CDS             2296..2553
                     /locus_tag="ACG8KR_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0201928.1"
                     /translation="MPIRILTALVNKITIRKAFYISSAFFLFSNQTYNHLFLFKNVGV
                     IQFCCKHLFYLHIVNGRTNNGFFLVSLYRILFPKKKKQLEI"
     gene            complement(2595..4019)
                     /locus_tag="ACG8KR_002616"
     CDS             complement(2595..4019)
                     /locus_tag="ACG8KR_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFQ0201929.1"
                     /translation="MWEKVEKKQGPIYRQIMEQIMREIENGRINPGSQLESERKLGQL
                     FGVNRSTVVHALEELRELGVLTSKRGSGWFVNQTEWGKFAVPRIDWRQMISPRYEQTD
                     WYEQKIREAKQIQKTSFLDLYASEMPDELLPNFEFPSYSLEEILAEEKEMDLLGYTPL
                     RNKIKRYLESKFELAFSPNQLLVTGGGQQAIFLILQTILSAGEGIAVESPSFFYRLPL
                     FKASGTRLFGIPMDEEGIDLEKLKESIHKNKLKAVLINPNFQNPTGKVMSQKRREKLV
                     ELCRQYRLPIIEDDVFTDLSFLNQEEVLPIRTIDPDNVLYVGSLSRVLGKTTKIGWVI
                     GPETFISQLAQAQEMMEFSMNILTQITVANVFNEAIDLKMQDVRKKLKEKSRLISAWG
                     DSQSFFKLHSILGGYYAWITWDGKKLDPELAEKVVDMGLGIAPSLLFGQDINGIRINY
                     SRLDENQIPLFYSKMKILEKWLTE"
     gene            complement(4283..5260)
                     /gene="guaC"
                     /locus_tag="ACG8KR_002617"
     CDS             complement(4283..5260)
                     /gene="guaC"
                     /locus_tag="ACG8KR_002617"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902560 - GMP reductase complex [Evidence
                     IEA];
                     GO_function: GO:0003920 - GMP reductase activity [Evidence
                     IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HFQ0201930.1"
                     /translation="MKVFDYEDIQLIPNKCIVNSRSECDTTVTLGKHTFKMPVVPANM
                     QTIIDETIAEFLAENGYFYIMHRFDEAARIPFIKKMKKRGLITSISVGVKKEEYSFIE
                     KLAEESLNPDYITIDIAHGHANSVIDMIQHIKKYLPETFVIAGNVGTPEAVRELENAG
                     ADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIAKS
                     VRFGATMVMIGSLFAGHEESPGETKVENGIVYKEYFGSASEFQKGEKRNVEGKKIWIQ
                     HKGSLKDTLVEMQQDLQSSISYAGGRDLEAIRKVDYVIVKNSIFNGDTI"
BASE COUNT         1465 a         1138 c          910 g         1802 t
ORIGIN      
        1 aatttaaatt gataagccat gactttgttt cgtcccttct tttcttaaaa ttccgcgatt
       61 tcttttactt tgtcttcgat atcttttgca tttggcaacc agacattttc tgcttgtcca
      121 aatgggaaga ctgtatcagg tgcagaaact cgtccgattg gggcttctaa tgaaaggata
      181 gcgcgttctg agatctcaga aactacttgc gcggcaacgc ccgcttgtcg ttgtgcttct
      241 tggacaacaa ctacgcgacc agttttttcc acggattgaa tgatcgtttc tacatctaat
      301 ggcgcaactg tacgaagatc aatgatttca acagatatat tttcttttgc aagattgtct
      361 gctgctttga ttgcttcacg caccattgct ccgtatgtga taatagatac atctgttccc
      421 tcacgagtaa ctgctgcttt atccaaagga acttcgtaag cttcatccgg cacttcttct
      481 cggaatgagc gatatagttt catgtgttct aggaaaacaa ccggatcgtt acttctaata
      541 gaagaaatca atagtccttt tgcatcataa gggttggaag gaatcactac tcgaatacca
      601 ggagactgag cgatcaaacc ttctaaatta tccgaatgca attctggtgt atgaacaccg
      661 ccaccaaatg gcgaacgaat tgtgatgggt agatttctag tgccgctcat tcgatatctt
      721 gtacgagcca tttgagctac tacttcgtcc attgcttcga aaataaaacc aaagaattgg
      781 atttcaggaa ctggacgaaa tccttctaac gcaagaccga aactcaatcc tgcgattcca
      841 gattcagcta aaggagtatc gaatactcgg tcttctccga acttttcctg caatccttca
      901 gttgcccgga aaactcctcc gtttttccca acatcttcac caaaaacaac gacattctcg
      961 tcattttcta gttcaagtgc taaggcatct gtgattgctt ggatcattgt tttttgtgcc
     1021 atgattattt cgactccttc gcttcataaa ttgcaatttg ttctttgatt gattgtggag
     1081 atacttcgaa catgtttttc aagaaatctg aaaccttttg ttttggtact ttgtcggctt
     1141 cagcaatagc tgctttgatt tcttctttag ttgcttcgat gacttgttct tctttttctt
     1201 cagaccaaag tcctttttcc gtcaaatatt tacggaagcg gatcaatgga tcttttttct
     1261 gccaatcgtc atctgtgtct tttgtacggt agcgggttgg atcgtcacca gatagtgtat
     1321 gtggtccata acgataagtc agtgtttcga ttaggactgg tccatttcca ctgatcgccc
     1381 attcccgtgc cattttagat acagtatata ctgcaagcgg atccattcca tcgacttgaa
     1441 taccaggaat cccagctgcg acagcttttt gtgcaagtgt ttttgctgca gtttgttttt
     1501 cacgaggtgt agagatcgca tacccgttgt tttggatata aaatacggca tttgcatggt
     1561 aagcgccagc gaaattgatt gcttcgtaaa aatcaccttg tgatgaaccg ccatcaccag
     1621 tataagtgaa gacgacattt ttcttgtttc gtttcttcaa acctaatgca acaccggctg
     1681 cttgtacata ttgtgcaccg atgataattt gtggtggtag agctttcaat tcttccggat
     1741 aagaattacc tgcagcatgt cctcgagacc ataaaaaagc ttgagataaa ggtaaaccat
     1801 gttttactag ttgaggtaca tcccgataac ctggaagaag aacatcttct ttatcaaatg
     1861 cgtaagcact agccagctga cttgcctctt gtccagcagt tggtgcaaaa aagcctaggc
     1921 gtccttggcg gttcagcgct gttgaacgtt ggtctaatac acgagaccat accatatgag
     1981 tcattaattc aacgagttca tcatcggata aatcaggtac taaatctttg ttaactacgt
     2041 ttccatcttg atcaagtgct tgatatactg ggaaatctgc attgacttca tctagcagtg
     2101 cttggaagtc gatcggtgct tttttgtttg ccatttgtca cacattcctc tctcatctat
     2161 aagaaaatct cttcattctg cgattgaaac agtgaataaa actgtatcac tatttaaccg
     2221 tcttcattaa taaattatca tggatttatg agaaatacaa gctaaaagca caaattttca
     2281 cgatgaaagg aaattatgcc aatccgcata ttaacagcat tagtgaataa aatcacaata
     2341 aggaaagcgt tttatatttc atctgcattt tttctatttt ctaatcaaac ttacaaccat
     2401 ctgtttctat ttaaaaacgt tggtgttata cagttttgtt gcaaacattt attttattta
     2461 cacatcgtga acggacgtac aaataatggt tttttcttag tttctttata tcgtattctt
     2521 tttccgaaaa aaaagaaaca gcttgaaatt taaatccaag ctgttatatt ctttaatcta
     2581 tttactttgt gaaattattc tgttaaccac ttttctagaa ttttcatctt tgaataaaac
     2641 aacggtattt ggttttcatc cagcctactg taattgattc tgattccatt tatatcttgc
     2701 ccaaaaagta gacttggtgc aattccaaga cccatatcta ctactttttc tgctagttcg
     2761 gggtctagtt ttttcccatc ccaagtaatc caggcatagt aaccacctaa aattgaatga
     2821 agcttgaaaa acgattgtga atctccccaa gcgcttatca gtcggctttt ttctttcaac
     2881 ttttttctta catcttgcat tttcaaatca attgcttcat taaatacatt agccactgtg
     2941 atttgtgtaa ggatattcat cgaaaactcc atcatttctt gcgcttgagc tagttgcgag
     3001 ataaaagtct caggtcctat cacccaaccg attttcgttg tttttcctaa aaccctagat
     3061 aacgaaccga cgtataacac attatccgga tcaatcgttc gtataggaag gacttcttct
     3121 tggtttagaa atgaaagatc cgtaaataca tcgtcttcaa taattggaag ccgatactgt
     3181 cgacacaatt ctactagctt ttctcgtcgt ttttgactca tcacttttcc tgtagggttt
     3241 tgaaaattcg ggttgataag cactgctttc aatttatttt tatggatcga ttctttcagt
     3301 ttttctaagt cgatcccttc ttcatccatc ggtatcccaa ataaacgcgt tcctgaagct
     3361 ttgaaaagag gtaatcggta aaaaaaagaa ggtgactcta cagctatccc ctctccagct
     3421 gaaagaatcg tttgtaaaat caaaaagatt gcttgctgac cgccaccagt cacaagaagc
     3481 tggttggggg aaaatgccaa ttcaaattta ctttccaaat accgtttgat tttgttacgt
     3541 aacggagtat agcctaaaag atccatttct ttttcttccg ctaagatttc ttcaagagaa
     3601 taagaaggaa actcaaaatt cggcaaaagc tcatctggca tctcgctagc gtataaatca
     3661 agaaacgatg ttttttggat ctgctttgct tctcttattt tttgctcata ccagtccgtc
     3721 tgctcataac gaggagagat catttgccgc caatcgattc ttggaacagc gaatttcccc
     3781 cattcagtct gattaacaaa ccatccgctg ccacgtttgc tcgttagtac cccaagctcc
     3841 ctaagctctt ccaatgcatg gacaacggtg gaacgattca caccaaaaag ttgccctagt
     3901 tttcgttctg attcaagttg acttccagga ttgatccttc cgttttcgat ttctctcata
     3961 atctgttcca taatttgacg ataaatcggt ccttgttttt tctctacttt ttcccacata
     4021 agcttctctt ttctattgtc tattttttct tttgatttta ccgaatttac gagaaaaaag
     4081 aaaaactttt tttgcaaaac ataagggact gtgacaaaaa tcttatcaca gtcccttatt
     4141 ctgaataatg atgttcaaaa gcttgtatct gcgataataa ttccagccaa acaaaacata
     4201 taaagaacta tttttgtttg gctgttctta ttacttaatg caggacaact ttttcacaac
     4261 atcctaatat tttatttatt ttttagattg tgtctccatt aaaaatcgaa tttttcacga
     4321 tgacatagtc aacttttcga atggcttcca gatctcttcc tccagcataa gaaatagatg
     4381 attgaaggtc ttgttgcatt tccactagtg tatctttcaa agatcctttg tgttggatcc
     4441 agattttctt tccttcaaca tttcgttttt cgcctttttg gaattctgaa gcagaaccaa
     4501 agtattcttt atacacgatt ccattttcga cttttgtttc gccaggagat tcctcgtgac
     4561 cggcaaacaa tgaaccaatc atgaccattg tcgcaccaaa acggacagat tttgcaatat
     4621 cgccatgtgt tctgatccca ccgtcagcga tgatcggctt gcgagctgct ttcgcacacc
     4681 atctaagagc tgccagttgc cagcctccag tcccaaagcc tgtcttgatt tttgtaatac
     4741 atacttttcc tggaccaatc ccgacttttg ttgcatctgc gcccgcattt tccaattcac
     4801 ggacggcttc cggtgtacca acattaccgg caatcacaaa agtttcaggc aaatattttt
     4861 taatatgctg aatcatatca ataactgagt tagcatgtcc atgcgcgatg tcgatagtga
     4921 tataatcagg atttagcgat tcttctgcca acttctctat aaaggaatat tcctctttct
     4981 tgacgccaac actgatcgag gtaatcaatc cacgcttttt catttttttg ataaacggaa
     5041 ttcgagctgc ttcatcaaac cgatgcatga tatagaaata accattttcc gctaagaatt
     5101 ctgcgattgt ttcatcaata atcgtctgca tgttggcagg cactactggc attttgaatg
     5161 tatgtttacc taatgtgacc gttgtatcac attcagaacg gctatttacg atacatttat
     5221 taggaattaa ttgaatatct tcgtaatcaa atactttcat tacattatcc tcctgatgtt
     5281 cacatccagc aaaacacgaa cattattaat ttaat
//
LOCUS       DAZKLD010000162         5311 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.164, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000162 DAZKLD010000000
VERSION     DAZKLD010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5311
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.164"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            5..361
                     /locus_tag="ACG8KR_002618"
     CDS             5..361
                     /locus_tag="ACG8KR_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine-rich SFCGS family protein"
                     /protein_id="HFQ0201931.1"
                     /translation="MIKVVIADRMGKGQNVAKGVEAAGGKAVVVPGMGADMRLGDVMQ
                     QEHADMGISFCGSGGAGALTAATKYGYPERHGMRSIEEGITAIKDGKTVLGFGFMDQE
                     ELGKRLTEEFIKKHGK"
     gene            377..727
                     /locus_tag="ACG8KR_002619"
     CDS             377..727
                     /locus_tag="ACG8KR_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4312 family protein"
                     /protein_id="HFQ0201932.1"
                     /translation="MSVQKTIQQKVIVSGKGETKQHAFASALAEIQNKIMEQSNVLLR
                     IEPVNVDILKAVENKYTERFLFFFFPRQRISYEVELSVTVAVTEIKLADISFSQQMTE
                     DPNGIAIPFVTKKI"
     gene            756..1535
                     /locus_tag="ACG8KR_002620"
     CDS             756..1535
                     /locus_tag="ACG8KR_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4311 domain-containing protein"
                     /protein_id="HFQ0201933.1"
                     /translation="MDFVIILVKSVIIGGLLGFAASVGAARMFHAPNTQGLGAFRTLG
                     EMNACMGDPASHFSFGLGFFFNAWASSVGAGAFTQDVTHRIIPNWAAAALLAKNKDVT
                     QTMHDPKKMGIIGAVIGAVVVAFLNTTAAAIPEALQVTAVSVLVPAATILINTVMPVI
                     FWLAALDAGKRTGFWGTLFGGIAQLIMGNAVPGVVLGILIGKGVDESGWNRVTKSMMI
                     AIVLLFIFSGFFRGFDMEMIKSFQLQVPNWLDNLHNIFSVK"
     gene            1546..2223
                     /locus_tag="ACG8KR_002621"
     CDS             1546..2223
                     /locus_tag="ACG8KR_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4310 family protein"
                     /protein_id="HFQ0201934.1"
                     /translation="METTEEQAKVLEAKRQKSFWFADWSFPLIVGIMSAAVFAGTHMY
                     VMYGVGAFNEVSIVAMLKAGLDGGSYGAAAAFGASFLFARILEGSLVGILDLGGSILT
                     GIGIGVPAILLSMKIAAPIDNFALSLLTGLVLGLIVGGIIVLIRKFTINQSNSTFGAD
                     VMMGAGNSSGRFLGPLIILSACAASIPIGIGATLGALGFYAWKKPIAGGAILGAMILG
                     AIFPAVN"
     gene            2242..3348
                     /locus_tag="ACG8KR_002622"
     CDS             2242..3348
                     /locus_tag="ACG8KR_002622"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase/deacetylase family
                     metallohydrolase"
                     /protein_id="HFQ0201935.1"
                     /translation="MFDTLIKNGRLVDGSKIEVAIEKGMIKAVAAEINQPAQEVVDLE
                     GKYYLSAGWIDDHVHCYEKMNLYYDYPDQIGVEKGVTTVIDAGTTGAENIREFYQLTK
                     SVKTNVYALMNISKWGIVKQDELADLTKIQEDLVSTALAELPEFIVGIKARMSKTVVG
                     ENGIKPLELAKKIQTENNDLPLMVHIGSAPPELEEVLSRMERGDVLTHCFNGKPNGIL
                     DPKTDQIKNFVWDAYNNGVVFDIGHGTDSFNFHVAETALKAGMKATSISTDIYIRNRT
                     NGPVFDLATTLEKLRVVGYSWEEIIEKVTAVPARNFHLKNKGQLAVNYDADLTIFEIV
                     ENEKELKDSNGFTRIAKEQILPIKTIIGGVIYDN"
     gene            3338..4429
                     /locus_tag="ACG8KR_002623"
     CDS             3338..4429
                     /locus_tag="ACG8KR_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DgaE family pyridoxal phosphate-dependent
                     ammonia lyase"
                     /protein_id="HFQ0201936.1"
                     /translation="MTISYGKFDLKEVINASGKMTILGVSKVSESVLAAQRFGGEHFF
                     EMSDLAIKTGAYLSQLLKVEDAQIVSSASAGIAQSVAALIGQGDMYHVYHPYTEKIAR
                     REIIIPKGHNVDYGTPVEVMVAQGGGKVIEAGYANMCTPEHVSMMITEKTAALLYIKS
                     HHAVQKSMLTVAEMVEVAKAHRLPLIVDAAAEEDLFKYSEMGVDLVIYSGAKAIEGPS
                     AGLVIGKKKYIQWIRLQSKGIGRAMKIGKDNILGFTQALEDYLKNGSESGDSMKQRLA
                     PFVNAINQLPDLSAKIVQDGAGRDIYRASVTINGDKSAKEVIQELKARNPAVYTREYQ
                     ANNGIIEFDIRSVSQNEMEKIIKRLREIM"
     gene            4442..5182
                     /locus_tag="ACG8KR_002624"
     CDS             4442..5182
                     /locus_tag="ACG8KR_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KDGP aldolase family protein"
                     /protein_id="HFQ0201937.1"
                     /translation="MSVIPNYYKNRVCLNVLAHSVENAKACYEAAEGHVVLGVLSKNY
                     ASDEAAIEDMKRYQDATNNALSVGLGAGDPNQSQMVSRLSGVLQPQHVNQVFTGVGTS
                     RALLGQNDTVINGLVSPTGKIGYVNIATGPLSSQAPAAEVPIETAIQMLQDMGGTSIK
                     YFPMKGLAHREEFKAVAEACAKYEFYLEPTGGIDLSNFEEILKIAIDAGVKKVIPHVY
                     SSIIDKETGDTRIGDVKILLSMVKKIIH"
BASE COUNT         1708 a          866 c         1265 g         1472 t
ORIGIN      
        1 aatcatgatt aaagtagtta ttgcagaccg tatgggtaaa ggacaaaatg tggccaaagg
       61 ggtagaagca gctggtggaa aagcagttgt cgtaccagga atgggggcag atatgcgttt
      121 gggcgatgtg atgcaacaag agcatgcaga tatgggtatc tctttttgtg gtagtggtgg
      181 tgcaggtgca ttaacagcag caacaaaata tggctatcca gaacgacatg gtatgcgttc
      241 gattgaagaa gggatcacag ctatcaagga tggcaaaact gttctaggtt tcggctttat
      301 ggatcaagag gaattaggga aacgtctgac agaagaattt atcaaaaagc atggaaaata
      361 gaaagaggga catgaaatga gcgttcaaaa gacaattcag caaaaagtta tcgtctccgg
      421 taaaggggaa acaaagcaac atgcttttgc atcagcgtta gcagaaattc aaaacaaaat
      481 aatggaacaa tcaaacgtat tattgcgaat cgaaccagtc aatgtggata ttttaaaggc
      541 ggtcgaaaac aaatacacag aacgctttct atttttcttc ttcccacggc aacgtattag
      601 ctatgaagtc gaactctctg ttactgtggc tgtaacagaa ataaaattgg cagacatatc
      661 gttcagccag caaatgactg aagatccaaa tgggattgcc attccatttg taactaaaaa
      721 aatctaattt tgaatcataa gggggaaaga ataacgtgga tttcgtaatc attctagtca
      781 aatcggtcat tatcggggga ttacttggtt ttgcagcatc tgtcggggca gctcgaatgt
      841 ttcatgcacc taatacacaa ggtttaggag cttttagaac attaggcgaa atgaatgcct
      901 gcatgggtga tccagcttct catttttcat ttggtttagg ctttttcttt aatgcatggg
      961 cttcttcagt tggtgctggt gcattcacac aagatgtgac gcatcgaatc attcccaatt
     1021 gggcagcggc agcacttcta gcaaaaaaca aagatgtcac ccagaccatg catgatccga
     1081 aaaaaatggg gatcattgga gcggttatcg gggcagtggt ggtagccttc ttaaacacaa
     1141 cagctgcagc aattccagag gcgttacaag tgacggcggt ttccgttttg gtaccagcag
     1201 cgacgattct gatcaataca gtaatgccag ttattttttg gttggcagct ttagacgcag
     1261 ggaaacgaac tggtttttgg ggcactcttt ttggtggtat cgcgcaattg attatgggaa
     1321 atgcggtacc tggtgttgtt ctaggaatct tgattggtaa aggcgtcgat gagagcggtt
     1381 ggaatcgcgt cacgaagagc atgatgatcg ctattgtttt attatttatt ttcagtggat
     1441 ttttccgtgg atttgatatg gaaatgatca agagcttcca gcttcaagtg cctaattggc
     1501 ttgataactt gcacaacatc tttagtgtga aataggagga gaatcatgga gacaacagaa
     1561 gaacaagcaa aagtattaga agcaaagcgg caaaagagct tttggtttgc agactggtct
     1621 tttccgctaa tcgtaggaat tatgtccgct gctgtatttg caggaacaca catgtatgtg
     1681 atgtatggtg tgggtgcatt caatgaagtt tcgatcgtgg cgatgttaaa agccgggctg
     1741 gatggcggat cctatggcgc agcggcagcg tttggtgcta gtttcttatt tgccagaatt
     1801 ttagaaggct ctttagttgg gatcttggac ttaggcggtt cgatcctaac tgggatcgga
     1861 attggggtac cagcaattct cttaagtatg aaaatagcgg caccaatcga taattttgca
     1921 ctctctttat tgactggttt ggttctaggt ttgattgttg gtggcatcat tgtcttgatt
     1981 cgtaaattta cgatcaatca atcaaattcc acttttggtg ctgacgtcat gatgggggca
     2041 ggaaattcat ctggacgttt tttgggtcca ttaattatat taagcgcgtg tgcagcatct
     2101 attccaatcg gcattggcgc tacattagga gcactaggat tctatgcttg gaaaaaacca
     2161 attgctggag gtgctatttt aggagcaatg attttaggag caatcttccc ggcagtaaat
     2221 taatagaaag agggtcatta aatgtttgat actttgatta aaaatggaag gttagttgat
     2281 ggatctaaaa tagaggtcgc tatagaaaaa ggcatgatta aagcagttgc agcagaaata
     2341 aatcagccgg cgcaagaagt agttgacttg gagggaaagt attatttatc cgctgggtgg
     2401 atcgatgatc atgtccactg ctatgaaaag atgaatcttt attatgacta tcctgatcag
     2461 ataggggtcg aaaaaggtgt gacgacggtc attgatgctg gaactacggg agcagaaaac
     2521 atccgtgaat tttatcaatt gacgaaatcg gtcaagacaa atgtgtatgc gctaatgaat
     2581 atctctaaat ggggcattgt caaacaagat gaacttgctg atctaacaaa gattcaagaa
     2641 gatctagtaa gtactgcgtt agcagaactg ccggaattta ttgtcgggat taaagcgaga
     2701 atgagtaaga cagttgttgg tgaaaatggc attaagccat tagaattagc taaaaaaatc
     2761 cagacagaaa acaatgatct gccattgatg gttcatatcg gttctgcacc tcctgagtta
     2821 gaagaagtat tgtcaagaat ggaaagaggc gatgtattga cccattgttt taatggaaaa
     2881 ccaaacggta ttttagatcc aaagacagat caaattaaaa actttgtctg ggatgcttac
     2941 aataatggtg tcgtctttga tattggacat gggacggata gctttaattt ccatgtggca
     3001 gaaaccgcac taaaagcagg aatgaaagct acctctatta gtacggatat ctacatccgc
     3061 aatcgtacaa atggcccagt ttttgatcta gcaactactt tagaaaaatt gagggtagtc
     3121 ggctattctt gggaagaaat cattgaaaaa gttacagcag ttcctgcacg gaattttcat
     3181 ttgaagaata agggccagtt agctgtcaat tatgatgcag atttgacgat atttgagatt
     3241 gttgaaaatg aaaaggaatt gaaagattcc aacggattta cacgtatagc taaagaacaa
     3301 attttaccga ttaaaacaat tattggaggc gtcatatatg acaattagct atggaaagtt
     3361 tgatttaaaa gaagtcatta atgcatcggg aaaaatgacg atcctagggg tttctaaggt
     3421 atctgaaagt gtcctagcag cacaacgatt tggaggcgag catttttttg agatgagtga
     3481 tcttgccatc aaaaccggcg cttatctttc tcaattactg aaagtggaag atgcgcaaat
     3541 cgtttcttct gcttcagcag ggattgctca atctgttgct gctttaatcg gacagggcga
     3601 catgtaccat gtctatcatc cctatacaga aaaaatcgct agacgggaaa tcatcatacc
     3661 taagggacat aatgtagatt atgggacacc ggttgaagta atggttgctc aaggtggagg
     3721 aaaagtcata gaagctggct atgcaaatat gtgtacaccg gaacatgttt ccatgatgat
     3781 tacggaaaaa actgctgcgc ttctatatat taaaagtcat catgcagttc agaaaagtat
     3841 gctgacggtg gcagaaatgg tagaagttgc caaagctcat cgtttgccat tgatcgttga
     3901 tgcagctgcg gaagaagatt tattcaaata tagcgaaatg ggcgttgact tagtgattta
     3961 ctctggggca aaagcaatcg aaggtccgag tgccggactg gtcataggaa aaaagaagta
     4021 tattcaatgg attcggctac agtctaaagg tattggccga gcaatgaaaa tcggtaaaga
     4081 taatatttta ggctttaccc aggcactaga agattattta aaaaatggga gcgaatccgg
     4141 tgactccatg aagcaacgat tagcgccttt tgttaacgca atcaatcaac ttcctgacct
     4201 ttcagcgaaa atagttcaag atggtgccgg cagagatatt tatcgagcaa gtgtaacgat
     4261 taatggcgat aaaagtgcga aagaagtcat tcaggaatta aaagcacgaa atccagcagt
     4321 ctacacccga gaatatcaag caaataacgg aatcattgag ttcgacattc gttccgtcag
     4381 ccaaaacgaa atggaaaaaa ttataaaaag attgagagaa atcatgtagg aggcttttaa
     4441 attgtcagtt atcccaaatt attataaaaa tcgtgtatgt ttaaatgtat tagctcattc
     4501 agttgaaaat gcaaaagcat gctatgaagc agcggaaggg catgtcgtac taggtgtgct
     4561 atcgaaaaat tatgctagcg atgaagcagc aatcgaagat atgaaaaggt accaagacgc
     4621 aaccaataat gcgttgtcag ttggattagg cgctggagat ccaaaccaaa gtcagatggt
     4681 ttcgcgttta tctggggttt tacaaccaca acatgtcaat caagtattta ctggcgtggg
     4741 gacttctcgt gctttattgg gccaaaatga tacagtcatc aatggattgg tttcaccaac
     4801 aggtaagatc ggctatgtca atattgctac aggtccgtta agttcacaag cgccagcagc
     4861 tgaagtgcct attgaaactg ccattcaaat gctgcaagac atgggaggta cttcaatcaa
     4921 gtatttcccg atgaaaggct tagcacatag ggaagaattt aaagcagttg cggaagcttg
     4981 cgcaaaatat gaattctatt tagagccaac aggaggaatt gatctttcaa actttgaaga
     5041 aattctgaaa attgcaatcg atgcaggtgt aaaaaaagtg attcctcatg tttatagttc
     5101 aattatagat aaagaaacag gagatacgcg tataggagac gtaaagatac tattaagcat
     5161 ggtaaaaaaa attattcact aactcattag aaaataagaa gcttattcca gaatgaaatt
     5221 aaatggaata agctttttat aattaattac ggaaattgtt aatgtagata ataatttaga
     5281 tgtacgtaag cagaagtaca gaaatgtaag a
//
LOCUS       DAZKLD010000163         5269 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.165, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000163 DAZKLD010000000
VERSION     DAZKLD010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5269)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5269)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5269
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.165"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(321..2087)
                     /locus_tag="ACG8KR_002625"
     CDS             complement(321..2087)
                     /locus_tag="ACG8KR_002625"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0201938.1"
                     /translation="MQMIGGLTMKSFYKNKFALSDQGAEALTKASISSFFVYCINMVP
                     AFIIMMLIDELVLENTKPRWLYFAVSFVTLLFMYWLLDREYENLYNSTYKESAHLRVQ
                     IADDLSNLPLSYFSKHNLSDLSQTIMSDVEGIEHAMSHAIPKAGGMALFFPFISVMLL
                     VGNVKMGLAVILPTLFSFVLILLSKKSQTKANTKYYDTLRENSEEFQETIELQQEINS
                     FNLSKKVQDRLFKKMEESERIHLKVELSTFSVMALSSIFSYVSLAVVILVGVHLLLTG
                     EVTILYVVGYLLAAIKIKDSFDSMKEAVLEIFYLAPKIQRIRAMKETSIQEGSDSPLK
                     SFDIELRDVSFSYDNNTPILDHISFTAKQGEVTALVGASGSGKTSILKLVSRLYDYDE
                     GCILIDGYDIKRVSPASLFSKIAIVFQEVTLFNTSILENIRIGNSQASDEEVKKAARL
                     ANCEDFIEKLPDGYHTLVGENGSSLSGGERQRLSIARAFLKNAPILILDEITASLDAE
                     NEKRIQESLNRLIQDKTVLIISHRLKSIEKVNKIVVMDQGKLVDQGTHNELYRRSEIY
                     KNLIKKTKLSEKFVYEKEAQSR"
     gene            complement(2047..3801)
                     /locus_tag="ACG8KR_002626"
     CDS             complement(2047..3801)
                     /locus_tag="ACG8KR_002626"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0201939.1"
                     /translation="MKVYKKLFSYVPKMKYYGWLATLFSDFSVVLTVYGYYSIYKFLQ
                     SLIIVGDEYLAKSYAVQTVAWLTLGALFYIFSGLFSHILGFRLETNLRKKGISGLTEA
                     SFRFFDLNSSGYIRKTIDDNAAKTHMAIAHLIPDSAQAIVTPVLSVALGFFINFKIGL
                     VLIAMLVIGVFLLKKMTENTNFIQKYQESLDVLSSETVEYIRGIQVIKIFGAKVTSFK
                     ALNKAINDYAKFAYKYSLSGKTPFVLFQWLFFGIVCILIVPITFFITSLTTPKILAVD
                     LLMLFFLSGIIFVSFMRIMYVSMHLFEARYAVETLDSLYLEMKQDQLKYGNETQIKNC
                     DIAFENVSFSYKEECVLENFNLVLEQKRIYALVGKSGSGKSTIAKLISGFYNVDKGQI
                     KIGGKPLNHYSKDTIINTIAFVFQDTKLFNQSIYDNVSIANPQAPASKVFDALRLAGC
                     ESILEKFPEREHTLIGSKGVYLSGGEKQRIAIARAILKDAKIVIMDEASASIDPDNEY
                     ELQKAFQHLMKEKTVIMIAHRLSSIKSVDEILVLSEGKIVERGNHNELMAKHQVYKQL
                     VTNYENANDWRLDNEKFL"
     gene            complement(3911..4480)
                     /locus_tag="ACG8KR_002627"
     CDS             complement(3911..4480)
                     /locus_tag="ACG8KR_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HFQ0201940.1"
                     /translation="MKTKDYIFIGLATVISLVIYFFSIMVSSIGGAFGHSISPGIFGL
                     LSGIIFVYISYNYPRKGIFTIYTIVLLLFFTLMGGAYLPWFISSISMAILADIILSVF
                     GYDRAIPQVASWALMQLGSAAGQWIPIWFFTDRFRQDWIDKGQSAATMDAMIHYAVGI
                     WGIISVLVVASLSMIGVLIGRKVLKKYKK"
     gene            complement(4498..>5269)
                     /locus_tag="ACG8KR_002628"
     CDS             complement(4498..>5269)
                     /locus_tag="ACG8KR_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFQ0201941.1"
                     /translation="NRTVKTTTIQLLKTENNNALRKFDIFNSSIEVPYIEKVSADVLK
                     VEEITYENILKDINFDVKVGDVIGLVGKNGVGKTTLLRILMKIIRPTKGKIIENKKNL
                     SSPFLVMQEMDYQFFTESVRSELALGNEIVSKDQQEKILKKMELYKMKDQLPFDLSGG
                     EKQRLLVSIASLSKTNLFLFDEPTSGLDYINMERIADLIKDLKEKGAVVIATHDPEFL
                     YKTCNRIIYLKNGGIKKDIKLRVEDSTLIEQIFNDITK"
BASE COUNT         1755 a          983 c          704 g         1827 t
ORIGIN      
        1 gctctgaaaa ataagaattc acttttatta gtccaatcaa acacaaatac cgttttaagt
       61 agcttttcct ctacttaaaa cggcaatatg aaagaaacta taacttttta ttcaatcttt
      121 tatgtttaac tggtttatcg ccaatttttt aaaactttga ttattcctta tattataaga
      181 atagacatta agtgaactaa ttcaatcata ctgattcttt cgtgtattca aaattagatg
      241 ctgtttaata aaattattct ttttgaaaaa gaattataac tatatcccaa aaattgtagg
      301 ttaatttaaa gcctatttca tcatcgtgat tgagcttctt tttcatagac gaatttctca
      361 gatagtttag ttttctttat gagatttttg tatatttctg accttcgata caactcatta
      421 tgagtacctt gatcaactag ttttccttga tccatcacta caattttatt taccttttca
      481 atagacttca agcgatgcga tataattagt actgtcttat cttgtattaa acgatttaaa
      541 ctttcctgaa tacgtttttc attttccgca tctaaactgg cagtaatttc atctaaaatt
      601 aaaattggcg catttttcaa aaaagcccgt gctatggata ggcgttgtcg ctctcctcca
      661 gaaagtgaac ttccgttttc tccgaccaat gtatgataac cgtcaggtag tttttcaata
      721 aagtcttcgc agttagctaa acgagctgct tttttcactt cctcatctga cgcttgacta
      781 tttccaatac gtatattttc caaaatactt gtattaaaca aagttacctc ttgaaaaaca
      841 atagcgattt tgctaaacaa agaagctgga gatacacgtt taatatcata cccatcaatt
      901 aaaatacagc cttcatcata atcatacagt ctagacacta atttcaagat actagttttc
      961 ccggaaccac tagcacctac taacgcagtc acttctcctt gtttagccgt aaaagagata
     1021 tgatctaaaa ttggggtatt gttatcatat gagaaagaga catctcgcaa ctcaatgtca
     1081 aaagatttca gaggactatc tgatccctct tggatactgg tttccttcat agctctaatt
     1141 cgctgtattt taggagctaa ataaaatatt tcaagcactg cttctttcat agaatcgaag
     1201 gaatccttta tttttattgc ggctagtaag taaccaacga cgtagagtat agtcacttca
     1261 cccgttaaca gtaagtgaac acctactaga atgactactg ctaaactaac atatgagaaa
     1321 atagaggata aggccatgac tgaaaaagta cttaattcta cctttaaatg aatcctttca
     1381 ctctcttcca tttttttgaa aagtctgtct tgaacttttt tagatagatt aaagctatta
     1441 atctcttgct gcaattctat agtttcttga aattcttccg agttttctct caaagtatcg
     1501 taatatttag tattggcttt cgtttgggat ttctttgata acaagattaa gacaaaacta
     1561 aataacgttg gcaaaataac agctaatccc attttgacat taccaaccaa aagcatcact
     1621 gaaataaaag ggaaaaacag agccataccg ccggctttag gtattgcatg actcatcgca
     1681 tgctcaatac cttcaacgtc agacatgata gtttgagata aatctgataa attatgtttt
     1741 gaaaaatagg ataatggtaa atttgacaaa tcgtctgcaa tttgcactct taaatgcgca
     1801 ctttctttat aagtactatt atataagttt tcatactccc tatctaacaa ccaatacatg
     1861 aaaagcaaag taacaaacga tacggcaaaa taaagccaac ggggctttgt attttctaaa
     1921 actaattcgt ctatcagcat cattataatg aatgctggta ccatattaat gcagtataca
     1981 aaaaaactag aaatagaagc tttagttaaa gcttctgcgc cctgatcact taaagcaaat
     2041 ttgtttttat aaaaactttt cattgtcaag cctccaatca tttgcattct cataattagt
     2101 gaccaattgt ttatacactt gatgtttagc cattaattca ttatgatttc cacgttctac
     2161 tattttccct tctgacaaga caagaatttc atctacagat ttaatgctgg ataatcgatg
     2221 agctatcata ataactgttt tttctttcat cagatgttga aaagcctttt gtagttcata
     2281 ctcattatct ggatcaatag atgcactggc ttcatccata ataacaatct ttgcatcttt
     2341 taaaattgct ctggcaatag ctatacgttg tttttctcct cctgacagat aaactccttt
     2401 actaccaatt aaggtatgct ctctttcggg aaatttttct aaaatcgatt cgcaaccggc
     2461 taatcttagc gcgtcaaata ctttacttgc tggcgcttga ggatttgcta tagaaacgtt
     2521 atcgtaaata ctctgattga atagtttcgt atcttgaaag acaaaagcaa ttgtgttaat
     2581 aattgtgtct tttgaatagt gatttaaagg cttaccacca attttaatct gtcccttatc
     2641 cacattgtag aaacctgaaa ttagtttagc tatcgtactc tttccgcttc ctgattttcc
     2701 tactaatgca taaattcttt tttgttctaa aactaagttg aaattttcta aaacacactc
     2761 ttctttgtat gaaaagctga cattttcaaa tgcaatgtca caatttttaa tctgtgtctc
     2821 gttcccatat tttagttggt cttgcttcat ttctaaataa agggagtcta gtgtttcgac
     2881 tgcatatctt gcttcaaaca agtgcatact tacatacatt attctcatga aagaaacaaa
     2941 aataattcca cttaaaaaaa acaacatcaa caagtcaaca gctaatatct tgggagtggt
     3001 aagagaagtt ataaaaaagg ttataggaac tatcaaaatg caaactatgc caaaaaatag
     3061 ccattgaaaa agaacaaaag gtgttttccc actcaaagaa tacttatatg caaacttagc
     3121 ataatcgtta attgctttat tcaaggcttt aaatgatgtt actttagcac caaatatctt
     3181 tataacttga attccgcgga tatattctac cgtttcagag cttaacacat ctaacgattc
     3241 ttgatatttt tgaataaaat ttgtattctc tgtcattttc tttaacaaga atacaccaat
     3301 tactaacatg gcgattagaa ccaatccaat tttaaagtta atgaaaaagc ctaaggcaac
     3361 tgataaaaca ggtgttacta tggcttgagc actatctgga atcaagtgag caattgccat
     3421 atgtgtttta gccgcgttgt catcaattgt ctttcgaata tatcccgaag aatttaaatc
     3481 aaaaaaacga aaacttgctt ctgttaaacc gctaattccc ttttttctca aattagtctc
     3541 taagcgaaac ccaaggatat gagaaaataa gccagaaaaa atataaaata aagctcctaa
     3601 agttaaccaa gcaacagttt gaaccgcata actcttagct aaatattcat ctcctacaat
     3661 aattagagac tgaagaaatt tgtaaatact gtaatatcca tagactgtta atacgacaga
     3721 aaaatcagaa aataaagtgg ctaaccatcc gtagtatttc attttaggga cataactaaa
     3781 taattttttg tatactttca ttttttcctc ccccgcattc aatttcttct cttattcgtt
     3841 tacaactgtt aatgctttcc gtaatagcgt aaattagaga tgtacaaaca gataaaaggt
     3901 aaactacgtt ctattttttg tattttttta agacttttct tccaatcaat acaccaatca
     3961 tagataaact tgctacaact aacactgaaa tgattcccca tatacctacc gcatagtgga
     4021 tcattgcatc cattgttgca gcagattgtc ctttatctat ccaatcttga cgaaaacgat
     4081 ctgtaaagaa ccatatagga atccactgtc cggcagcact gcctaattgc attagagccc
     4141 aagacgcaac ttgaggaatt gctcgatcat atccaaatac agatagaata atatcagcta
     4201 agatcgccat actaatcgaa ctgataaacc aaggtaagta cgccccgccc attaacgtaa
     4261 aaaataacaa taaaacaatt gtgtaaatag taaaaattcc ttttctaggg tagttatagc
     4321 taatataaac aaatatgatt ccagataata atccaaaaat cccgggggat atgctgtgac
     4381 caaacgcccc accaatacta ctaaccatta ttgaaaaaaa gtaaattact agggaaataa
     4441 ctgtagctaa accaataaaa atataatctt ttgttttcat atatattctc ctcaattcta
     4501 tttcgttatg tcattaaaaa tctgttctat caaagttgaa tcttctactc taagttttat
     4561 atcttttttt attccaccat tttttaaata aataattcta ttgcatgttt tataaagaaa
     4621 ttccggatca tgagtagcta taactactgc acccttttct tttaagtctt taataaggtc
     4681 agcaatcctt tccatattaa tataatccaa cccacttgta ggttcatcaa atagaaataa
     4741 attagttttt gacaatgaag ctatagaaac caataatctt tgtttttctc caccggataa
     4801 atcaaacggt aattgatctt tcattttata aagttccatt ttttttaaaa ttttttcttg
     4861 ctgatcttta ctaactattt cattccccag ggctaactcg ctcctcactg attctgtgaa
     4921 aaattggtaa tccatttctt gcattactaa aaacggcgat gacaaatttt ttttattttc
     4981 gattatcttt cctttggtag ggcgaataat cttcatgagt attcttagta aggttgtttt
     5041 ccccacaccg tttttcccta ccaaaccaat aacatctcca actttgacat caaaatttat
     5101 gtcttttaga atattttcat aagtaatttc ttcaactttt aatacatctg cacttacctt
     5161 ttcgatatag ggaacttcaa tcgaactatt aaaaatgtca aatttcctta atgcgttatt
     5221 gttttcagtt tttaacaact gaatagttgt agtttttaca gttctattt
//
LOCUS       DAZKLD010000164         5112 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.166, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000164 DAZKLD010000000
VERSION     DAZKLD010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5112)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5112)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5112
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.166"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(275..445)
                     /locus_tag="ACG8KR_002629"
     CDS             complement(275..445)
                     /locus_tag="ACG8KR_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0201942.1"
                     /translation="MTKKHLLFGSILLGILSFSPMLLSVANKGNKKGELKLNPNYYNS
                     QNEEVFVEKLDK"
     gene            complement(460..993)
                     /locus_tag="ACG8KR_002630"
     CDS             complement(460..993)
                     /locus_tag="ACG8KR_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HFQ0201943.1"
                     /translation="MQPQETFTIKELSDLFKMSRQAISKHIQKLDSSMIAKNERGYKV
                     VLRSGVLQLARNLDNQRLLDKLHKHEQNNDKNAYIPNKNVVANKLLDQLREENEFLRE
                     QLALKEQNNSHIQKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENSSTTLSEATE
                     TMDEQTKNWKKWWQLWK"
     gene            complement(1014..1958)
                     /locus_tag="ACG8KR_002631"
     CDS             complement(1014..1958)
                     /locus_tag="ACG8KR_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HFQ0201944.1"
                     /translation="MANEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMREKGDKTVR
                     FTFDQLKDLSNYKATANVRFVEDLKATYEKILSLRFGRRSESGLSYDMFVMFTEFKIN
                     GDVENPYVDIRIYEKALPLLNNLDEWVRYSLQQFNELQSGYAKTMFRLLKQYRTKGFA
                     YFSKEDFLELLDIPKSYKQPDIDKRVIKPIRQELTAIFKGLTIKKKYGKGRGKPVIGY
                     QFTFKPEIKKADDFNKHAPTKEDQNKALALADKQKEYEEPATTINEKIKQKKRELELL
                     QQIKQLEKEKKALEKQTESALSEKEKKSLLSDLDNLFQ"
     gene            2595..2681
                     /locus_tag="ACG8KR_002632"
     CDS             2595..2681
                     /locus_tag="ACG8KR_002632"
                     /inference="COORDINATES: protein motif:HMM:NF033608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HFQ0201945.1"
                     /translation="MFELFICPLLIEVCVSLFSYWLNNRNKK"
     gene            complement(2802..3491)
                     /locus_tag="ACG8KR_002633"
     CDS             complement(2802..3491)
                     /locus_tag="ACG8KR_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0201946.1"
                     /translation="MAFYLPYLLIFASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLP
                     GVIFTLSIVLSGLFGVLEGNKRISRSILLGDITQKYEAACDKKKKEQVLTQKMEEFTT
                     CYEEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYFKDTQT
                     EEACYNLFSDTIPFSTSEQERKERQQSVTYERYKALLQQATNIQNPTKKETALNYLKS
                     VKEWLDQQQQN"
     gene            complement(3498..3776)
                     /locus_tag="ACG8KR_002634"
     CDS             complement(3498..3776)
                     /locus_tag="ACG8KR_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0201947.1"
                     /translation="MKEEQTTNKNYRSKTLQDIENEIKKQRAFLAPYTKERIQTTIDS
                     QVKKFHHALLNQRNKEQKKVIMQRSMDILAPLCCLIMTLLAFWLVWGE"
     gene            complement(3736..4650)
                     /locus_tag="ACG8KR_002635"
     CDS             complement(3736..4650)
                     /locus_tag="ACG8KR_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HFQ0201948.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIR
                     MLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHN
                     HIVINSVNLETGNKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKPEKRTMSEIKL
                     KEKNEPVWKDEIRFAIDQTMKNKKTRTYNQFCDSLKTFGIHCLNRGKNFTYELIHKKK
                     KVRSNKLGKDYEKETILHELDRRERAYNQQRTNELRRARASIRETAKEVRREQRTLTT
                     EQPEIRPTRRNHDQHVERGADYQQELSF"
     gene            complement(4632..5012)
                     /locus_tag="ACG8KR_002636"
     CDS             complement(4632..5012)
                     /locus_tag="ACG8KR_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HFQ0201949.1"
                     /translation="MSEQNQYLASNHSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVDPG
                     DAQRLSENLKIMQKELQKIWQQLK"
BASE COUNT         1296 a          944 c          925 g         1947 t
ORIGIN      
        1 tatactacca agtaacgcct gtaccaaggg atccaggcgc tgattttgtg aatttttatt
       61 ggtctaggtt aaaagctctt tttaaggctt ggttttctgc ggttgtttct ttctaattct
      121 ttgtgaatat tgatcggtca ttttttaaat acttgttatt tttgtttgtg tttgttatgc
      181 tttttacata tctcatattc atttattatt cttttctaag gttaataata ggataatctc
      241 tttcagaaag actgctcgta cgagcagtct tttcttattt atcaagtttt tctacaaata
      301 cttcctcatt ttgactgttg tagtaattag gattgagttt tagttcccct tttttatttc
      361 ctttgttggc cactgataat aacattgggc taaagcttaa tattcctagt aggatagaac
      421 cgaatagcaa gtgttttttt gtcataagta tttccttctt catttccata gctgccacca
      481 ttttttccaa ttctttgttt gttcgtccat tgtttctgtt gcttcagaaa gagtcgttga
      541 actattctct gaagactctt ccgtataagt caactgcagt tgctcctgaa gtttttcgat
      601 ttgttggttg gcttgcaggg tgagttgttg ttgctggtcg agtagttttt gtatatgaga
      661 attattttgc tctttgaggg caagttgttc tcgtaaaaat tcattttctt cacgcaactg
      721 atctagcaac ttgtttgcaa ccacgttttt gtttggtata taagcatttt tatcgttgtt
      781 ttgttcatgt ttatgcaact tatctagcaa cctttgattg tctaagttgc gagcaagttg
      841 caatactcca gaacgcaata caaccttata accacgctca tttttagcta tcatggaaga
      901 atctaatttt tggatatgtt tcgatattgc ttgcctactc atcttaaaaa gatctgacaa
      961 ttcctttatt gtaaaggttt cttgtggttg catactggtt gcctcctagg ttgctactgg
     1021 aataaattat ctaaatcaga taataaactt tttttctctt tttccgaaag tgcggactct
     1081 gtttgttttt caagagcttt tttctctttt tctagttgtt tgatttgttg gaggagttca
     1141 agttctcgtt tcttctgttt gattttttcg ttaattgttg tagcgggttc ttcgtattct
     1201 ttttgtttat cagctaaagc caatgcttta ttttggtctt ctttggttgg tgcatgtttg
     1261 ttaaagtcgt ctgctttttt gatctcaggt ttgaacgtaa attgatagcc aataacaggt
     1321 tttccccgtc cttttccgta tttctttttg attgttagtc ctttgaagat tgcagttagt
     1381 tcttgacgta taggttttat aactcgttta tctatatcag gttgcttata acttttagga
     1441 atatctaata attcaagaaa gtcttctttg gagaaatatg cgaaaccttt ggttctgtat
     1501 tgctttaaca gacgaaacat agtctttgca tagccacttt gtagctcgtt gaattgttgt
     1561 aaagaatagc gtacccactc gtctaaatta tttaacaaag gcagtgcttt ttcgtaaatt
     1621 cgtatatcta cgtaaggatt ttctacgtcc ccgttgattt taaattctgt aaacattaca
     1681 aacatatcgt aagaaagccc agactcactt ctacgaccaa agcgtaaaga taagattttt
     1741 tcataagttg cttttaaatc ttctacaaat cgtacatttg cagttgcttt gtagttactt
     1801 aaatctttta gttggtcaaa cgtaaaacga actgtcttat cccctttttc acgcattcta
     1861 gacacgatgg aaaaaaacaa attcatttca accggtgtaa attttcttaa aggaattgta
     1921 tttaattcat ggtggtattt tacaatctca ttagccatat aatcacctca atccaattat
     1981 gaatgacatt ttttaaatgt caagacattc atatttgacg ttcaaacaga caaaatgtcc
     2041 ttcataaaca gacaaaatgt ccttcataaa cagacaaaat gtccttcata aacagacaaa
     2101 atgtccttca taacattctg tatccattgg tagagtaagg acacagctta cggtaaagag
     2161 gtttaaaaag aggtttaaaa agagatataa aaagaggggg gctaattttc ccgcctaaaa
     2221 accacttctt ttttctttaa acaggttcgc gcatttttca aaactgccaa tccaaaacct
     2281 tgcttcctac ggtcgccaaa aaggtgtgat cgctacgctc tcacattgta ctgcgcagtc
     2341 gcctaaaggc tcctttgcta aaaacatgtt ggtaaagagg cctttgagcg gttctgaatg
     2401 cttatctaag ccgttttaag acagtcttgg ataaaaatcc ttatctagct ttaaagcgcc
     2461 ttacaggcgg tcagggcaag ccctaacaac cctaaaataa gcgatactta aaaacaccac
     2521 acaagtttaa ttttttaaga tataataact tgtcgcctat ccgacagtct tgcgactgga
     2581 aggaggtgta tctaatgttt gaattgttca tttgccccct tcttatagaa gtttgtgtct
     2641 ctttattcag ttattggttg aataaccgaa acaagaagta aaatcaataa aggcgacatc
     2701 aacctgaaaa aaagccccag agaatccgac ctctctgggg ctttcgtgta tctaatgttt
     2761 gatcatttgc atgtttagtt taacatgatt gccgatcaaa ttcaattttg ctgttgctga
     2821 tctaaccatt ccttgacact ttttagataa tttaatgccg tttctttttt cgttggattt
     2881 tgtatgttcg ttgcttgctg aagcagtgct ttataacgtt cgtaggtaac tgattgttgc
     2941 cgttcttttc gttcctgttc actagttgaa aaagggattg tatcactgaa taaattataa
     3001 caagcttctt ctgtctgcgt atctttaaaa tattgcacgt cttttttggt tcgctcatag
     3061 ttgtcttgaa cctctttttg ctgtttttct ggcaagtttt gataaagacg agtgagtttt
     3121 tccatgtttt tatccgtcaa acgtttttct tgattcacaa aaatatcgtt catttcctca
     3181 taacaagtgg taaactcttc cattttttgt gttaacactt gctccttttt ctttttatca
     3241 catgctgctt catacttttg agtgatatcc cctaataaaa tacttcttga aatacgttta
     3301 tttccctcta aaacgccgaa taaaccactt aaaacgattg ataatgtaaa tataacccct
     3361 ggcaataaaa acctcttaaa tcgaatttta gagcctttta atgaatatct tgtttgataa
     3421 gccttatagc taagccaaat aattcctaaa atactggcaa aaatgagtag atagggtaaa
     3481 tagaatgcca tttctattta ttctccccat accagccaaa aggctaacag tgtcatgatt
     3541 aagcaacata agggggcaag aatatccatt gatcgctgca taattacttt tttctgttct
     3601 ttatttcgct ggtttaatag cgcatggtgg aattttttga cttgtgaatc aattgtagtt
     3661 tgtattcttt ctttggtata aggtgcaaga aaagcacgtt gtttctttat ttcattttct
     3721 atgtcttgta acgttttaga acgataattc ttgttggtag tctgctcctc tttcaacgtg
     3781 ttggtcatgg tttcttcgag tcggtctaat ttccggttgt tcagtcgtaa gagttcgttg
     3841 ttctcttcgt acttcttttg cagtttcgcg aatgctagct cgagctcttc gtagctcgtt
     3901 ggttcgttgt tgattgtagg ctcgttctcg tctgtccagc tcatgtaaaa tcgtctcctt
     3961 ttcgtaatcc tttcctagtt tgttggatcg tacttttttc tttttgtgga tcaattcgta
     4021 agtaaaattt ttcccacgat tcaagcaatg aatgccgaat gttttaagag aatcacaaaa
     4081 ttggttatac gttcgagttt ttttgttttt cattgtttga tcaatcgcaa accgaatttc
     4141 gtctttccaa acaggttcat ttttttcttt taatttgatt tcagacatgg ttcgtttttc
     4201 gggtttcttc cctcgttcag tgattgttaa gtcatgttca agacaaatct cgtcatttag
     4261 gtcataacat ttgtttaaaa atgcttgacc atgggcttga aatttgttcc cagtttccag
     4321 attgacggag ttaatgacaa tgtggttatg aatatggtct ttgtctgtat gtgtgtaaat
     4381 cgcgatttgg tggtcagggg ctattttttt cgcatactct gcccctagtt gattggcttt
     4441 ttcagcggta atttctcctg gtcgaaaaga ctggatcaat aagtgtgctt gtacacgatc
     4501 gtcttttcca tacagcatgc gaatttgttt catttcgctt ttggcataat caatgtcaca
     4561 gtttatccct gttttgacgg ttgctcgtgg ttcggcgtaa ttgatcgctc ggctacaaga
     4621 agtggttcgg cttactttaa ctgttgccat atcttttgta gctccttttg catgattttt
     4681 aggttttcgg ataagcgttg ggcgtcgcca ggatcaacgt cgtgttggtg tgtgttgcac
     4741 cacttggcaa tttgattgat attcccgcca gctttggcta aatcgcgcgc gattgcctga
     4801 gcaatgtctg gcgccacttt cggtgtcacc agacgcgcgt tttgtgcctt ggctttcacg
     4861 aaagccggca cactcatttg caaagcttca gctgactgct tcagctttgc aaattcggat
     4921 tcgctcactc gaaaactgat ttgttttggc tcggatcgat aggtatattt tttagaatga
     4981 ttgctagcca aatattgatt ttgttcgctc ataagtccca ccctttcagt acgtcctacg
     5041 gacgtatcgt tcgcaatacc attgtaccta aaaaggtaca ataagtaaag ctcactaaag
     5101 ctcactaaag gg
//
LOCUS       DAZKLD010000165         4973 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.167, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000165 DAZKLD010000000
VERSION     DAZKLD010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4973)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4973)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4973
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.167"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            330..1253
                     /gene="truB"
                     /locus_tag="ACG8KR_002637"
     CDS             330..1253
                     /gene="truB"
                     /locus_tag="ACG8KR_002637"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HFQ0201950.1"
                     /translation="MDGLLTLWKERGMTSHDCVFKLRKILHTKKIGHGGTLDPDVDGV
                     LPICIGKATKVIEYLTDSGKIYVGEITLGFSTTTEDRSGERVETKPVDMSLSEEKIDE
                     VMASFIGEITQIPPMYSAVKVNGKRLYEYARNNETVERPVRKAQIYRFIRTSELTRDE
                     VTGTISWRFEVECGKGTYVRTLAVDTGKKLGYPAHMSDLTRTASAGMSQKDALTLEEV
                     AQAVKNGTIDDCLLPIEKGVKRFKRVDITDEVWQKVKNGMRLNYTVFALPEMPSEEIA
                     LFYKGKVVSIYQPNPKEKNKLKPSKVLRNEE"
     gene            1257..2198
                     /gene="ribF"
                     /locus_tag="ACG8KR_002638"
     CDS             1257..2198
                     /gene="ribF"
                     /locus_tag="ACG8KR_002638"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006747 - FAD biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009398 - FMN biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HFQ0201951.1"
                     /translation="MNIIKIRHPYQSEQIPEDDVVLVLGFFDGVHIGHQKVIKTGMEI
                     AQKEGLKLALMTFNQHPSIVFKKIDPSSVKYLTSLKQKEEKMAALGIDYLYEVDFTSS
                     FAHLAPQEFVDQYIVGLHAKYAVSGFDYTYGPKDIADVAHFPEYAKGRFKIVTVSKEE
                     EQGEKISSTRIRGLLDSGQVKEAAKLLGCTYEVEGVVVHGDARGRLLGFPTANVKVKS
                     TVHLPKEGVYVSEIKIGEIWYPAMGSIGHNDTFGEGRELTVELYILDFNQDIYGEHVA
                     VRWNQFLRDQVKFSSVEELIEQLKEDEQATAEYFSQQ"
     gene            2593..3378
                     /locus_tag="ACG8KR_002639"
     CDS             2593..3378
                     /locus_tag="ACG8KR_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFQ0201952.1"
                     /translation="MYKELENKVAVVTGGSKGIGTAIAQRFGKEKMNVVVNYNSDKEG
                     AEKAVKAIEEAGGKAVSVQADVSSEEGIQRLLDTALENFGTLDVWINNAGMENQHPTH
                     ELSLEDWERVLKVNLTGVFIGTKAALNYFTENDKKGNIINMSSVHEQIPWPTFAHYAA
                     SKGGVKLFTETVAMEYAPKGIRINSIGPGAIRTPINAAKFDDPEQKELLESMIPLGRV
                     GEPKEIAATAAWLASDESSYVTGITLFADGGMTLYPSFQGGKG"
     gene            3391..4242
                     /locus_tag="ACG8KR_002640"
     CDS             3391..4242
                     /locus_tag="ACG8KR_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GRP family sugar transporter"
                     /protein_id="HFQ0201953.1"
                     /translation="MGLLIALIPAVGWGIQPLILKKIGGRPTNEILGTGIGAVLVGLL
                     VQLFASPAGISVTTFLISLLSGAFWVIGQTGQYTSFNLIGVSKTMPISTALQLVGTSL
                     IGVFAFGEWSGSMGKIIGAAAIILLVFGSALTAISDDPGKKGGITKGISLLASTSIGY
                     WVYSALPKLVDASGLSIFFPQMIGVFLGAVIYVIFRKPTAFSEDKSWKATIVGAIFSV
                     SALAYIFSANENGVATAYIITQLNVVISTLGGMYILHENKTPKEKKLTIIGLLLIVVG
                     SMVTVFI"
     gene            complement(4366..4818)
                     /locus_tag="ACG8KR_002641"
     CDS             complement(4366..4818)
                     /locus_tag="ACG8KR_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0201954.1"
                     /translation="MKDFYSVNELAEQLGVTTRSIRNYLHEGKLKGTKVGGQWKFSER
                     NLFEFLYGDQAEEAAKEMQRFMLNAPITMRFNLQYRDFTAINQIREQLVQYHNDVYAN
                     KKDRLLQYDLYKDNHAEILIGGNFNHVVNFSQWINEKLLMQTDISLVS"
BASE COUNT         1681 a          815 c         1038 g         1439 t
ORIGIN      
        1 gattaagaaa ctgaaaaaat aaataaaaaa aaacggaaaa aagactggaa gaatggacag
       61 tctttttttt cgtttttggg aagtgaaaac caaactattt acctgtaagc cacccataaa
      121 tccaatcaaa tggtgcaaag aaaaaatctt ccattctatt ccctccttta aaaaaaacat
      181 aacatgattt ctatttacgt gagcatgaat tttgttacgt ttttattgcc gaaaaataaa
      241 aaccgttcat tatgatgtca aattcgtcag taattgatgc aatgtgttat aataattcag
      301 ttaaatcaaa aatgaaatgg agaaaagaca tggacgggtt attaacttta tggaaagaac
      361 gcggaatgac aagtcatgat tgcgtattca aattaagaaa aatcttacat acgaaaaaaa
      421 tcggacatgg gggtacgctt gacccagacg tagacggtgt gttgcccatt tgtattggaa
      481 aagccacaaa agttatcgaa tatttgacag attctgggaa aatctatgta ggcgagataa
      541 ctctaggttt cagtacaacg acggaagatc gatccggaga acgggtagaa acaaaaccag
      601 tagatatgtc tttatctgaa gaaaaaatag atgaagtgat ggcttcattt attggtgaga
      661 tcacccagat accaccaatg tactcagcgg taaaagtcaa cggtaagcgg ttatatgaat
      721 atgcccgcaa taatgaaaca gtggagcgtc ctgttagaaa ggctcagatt taccgtttta
      781 tccggacaag tgaattgaca agagacgaag tgacaggaac catttcttgg cgcttcgaag
      841 tagaatgcgg gaaaggaacc tatgtccgca cattagctgt agatacaggc aagaaacttg
      901 gttaccctgc gcacatgtct gatctgaccc gaacagcaag tgccggtatg tcccagaaag
      961 atgctctgac attagaagaa gtagctcaag ctgtgaaaaa tgggacaatc gatgattgtt
     1021 tgctgccaat agaaaaagga gtgaaacgat tcaaacgagt agatattact gatgaagtat
     1081 ggcagaaagt aaaaaacggt atgagactta attatactgt atttgcgctc ccagaaatgc
     1141 cgtcagaaga aattgctttg ttttataaag gcaaagttgt cagtatctat cagccaaacc
     1201 cgaaagaaaa aaacaaatta aaacctagta aagtattgag aaatgaggaa taacgaatga
     1261 atatcatcaa gatcagacat ccttaccaat cagaacaaat tccagaagac gatgtcgttt
     1321 tagtactagg attttttgac ggcgtgcata ttggacacca gaaagtcata aagacaggta
     1381 tggaaattgc acaaaaagaa ggattgaaat tagcattgat gactttcaat cagcatccat
     1441 cgattgtctt taaaaagatc gatcctagca gcgtgaaata tttgactagc cttaagcaaa
     1501 aagaagaaaa aatggccgct ttgggtatag attatttata tgaagttgat tttacttctt
     1561 cttttgctca tttagcaccg caggaatttg ttgatcaata catcgtaggg ttacatgcaa
     1621 aatatgctgt ttccggtttt gattatacgt atggaccaaa agacattgca gatgtcgctc
     1681 attttcctga atatgccaaa ggaagattca aaatcgtcac agtttctaaa gaagaagaac
     1741 aaggtgaaaa aatcagctct acccgtattc gaggattact agacagcgga caagtaaaag
     1801 aagcagcaaa actattaggt tgcacatatg aggtagaagg ggtagttgtc catggcgatg
     1861 cacgaggcag actactagga ttccctacag caaatgtcaa agtaaagagc acagttcatt
     1921 tgcctaaaga aggtgtttat gtatcggaaa taaaaattgg ggaaatttgg tatccagcca
     1981 tgggttcaat tggacataat gatacattcg gtgaaggacg ggagctgact gtagaattgt
     2041 atattttgga ctttaatcaa gatatctatg gtgaacatgt cgcagtacgc tggaatcaat
     2101 ttttaagaga tcaagtgaaa ttttctagtg tagaggaatt gattgaacaa ctcaaagagg
     2161 atgaacaagc aacagctgaa tatttttctc agcaataaaa cattatgcat aaaatctctt
     2221 gtttatgtat aactagcaat ttcaataaaa tgggtgataa ataagcccag gaaaggggct
     2281 gtgacaaaag tcttgtcaca gccttttttt ccaaataaat ggtttttcag aagtagcttc
     2341 ttcggaaata agccgaaatt tcacaaaaat ttgagaagta atttttgtaa attacttctt
     2401 atttcttgaa gctggccact tctgtcacaa cctctttctt tatagaaata aaaggacact
     2461 agcaattcat gaaaacgatt gctaaagcgt atcatttgct ttagactata tatcttggtg
     2521 tacactcact ttaagacgga aataaaagtt atccgtaata acaggtttag gtatgaaaaa
     2581 ggagtgggta ctatgtataa ggaattagaa aacaaagtcg cagtagtaac tggtggctca
     2641 aaggggattg gaacagcgat tgctcaacga ttcgggaaag aaaaaatgaa tgtagttgtc
     2701 aactataact cggataaaga aggagcagaa aaagcagtca aagcaatcga agaggcaggc
     2761 ggaaaagcag tctctgtaca agcagatgtc agttctgaag aaggaataca gcgtttactt
     2821 gatacggctt tagaaaattt tgggacactg gacgtttgga ttaataatgc tgggatggaa
     2881 aatcagcatc caacccatga acttagcctt gaagattggg aaagagtatt aaaagtcaat
     2941 ttgactggcg tttttatcgg aacgaaagcg gctttaaatt actttacaga gaatgataaa
     3001 aaaggaaata tcatcaatat gtcttctgtt catgaacaaa tcccatggcc aacgtttgct
     3061 cattacgctg cttcaaaagg cggagtaaaa ctattcaccg aaacggtagc aatggagtac
     3121 gcaccaaagg gaatcagaat caatagtata ggacctggtg ctatccgtac acctattaat
     3181 gcggcaaaat ttgatgatcc tgaacaaaaa gaactcttgg aaagtatgat cccactagga
     3241 cgcgtaggtg aaccaaaaga aatagcggcg acagctgcat ggttggcttc agacgaatcc
     3301 agttatgtta caggtattac actatttgca gatggtggga tgactttata tccttctttc
     3361 caaggtggaa aaggataagg gtgaataaaa atgggtttat tgattgcatt gatcccggca
     3421 gttgggtggg ggatccagcc actgattttg aaaaagattg gtggcagacc aacaaatgag
     3481 atattaggaa ctggtattgg agccgtgctc gttggcttac ttgttcaatt attcgcttct
     3541 ccagctggta tttcagtgac tacatttttg attagtttac tttctggtgc attctgggtc
     3601 atcggacaaa caggacagta tacatctttc aacttgattg gtgtttctaa aacgatgcca
     3661 atttcgactg cattacagtt agtcggaacc tcattgattg gtgtcttcgc ttttggagaa
     3721 tggtctggaa gtatgggaaa aatcattggt gcagcagcaa tcattcttct tgtatttggt
     3781 tctgctttaa cggcaatcag tgatgatcct ggaaaaaaag gcggtatcac aaaaggaatc
     3841 agtctattag catcaacaag tattggttat tgggtttaca gtgcattgcc caaactagtt
     3901 gatgcaagcg gactttccat tttctttccg caaatgatcg gtgtcttttt aggggcagtg
     3961 atttatgtga ttttccgaaa accaaccgca ttttctgaag acaagagttg gaaagcaaca
     4021 atagttggag ccatcttcag tgtgtcagca ttggcttata ttttttcagc aaatgaaaat
     4081 ggggttgcta ctgcatatat cattacacag ctaaacgtag ttatctctac attaggcggt
     4141 atgtacatcc tgcatgaaaa taagacacca aaagaaaaaa aattaacgat tatcggatta
     4201 ttgctgatcg tagtaggaag catggtcacg gtctttattt gaggataaag atggtaaaat
     4261 ctcatttatt aaaaaaatat ttgtttgaac caaacagtaa gtacttataa aaaaatagcg
     4321 ctagaatttc caacatctct ggaatttcta gcgcttttta attgtttaag atacaagtga
     4381 aatatctgtc tgcatcaata atttttcatt gatccattga ctaaaattta cgacatgatt
     4441 gaagttacca ccgattagta tctccgcatg attgtcttta taaagatcgt attgtaaaag
     4501 acggtctttt ttatttgcgt aaacatcgtt atggtactgg actaattgtt ctcggatttg
     4561 attgattgct gtaaaatcac gatactgaag gttgaaacgc atcgtgattg gtgcatttag
     4621 catgaatcgc tgcatttctt ttgctgcttc ctctgcttga tctccgtata ggaactcaaa
     4681 aagattacgt tcagaaaatt tccattgccc gccaaccttt gttcctttta gttttccttc
     4741 atgaagataa tttctgatag aacgtgtagt aacacctaat tgttcggcta attcatttac
     4801 tgagtaaaaa tctttcaatg ttcaccctcc tatgtgaatg gttctactga cttagttgat
     4861 atttgcttaa tcacgaaatg tagaaagaac gattttaatt aaggtcattg taaaaatcaa
     4921 gtcagaaaat tctaactttt ctttctctaa agaaattcgt tctctcctaa agt
//
LOCUS       DAZKLD010000166         4805 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.168, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000166 DAZKLD010000000
VERSION     DAZKLD010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4805)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4805)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4805
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.168"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(51..1199)
                     /gene="nagA"
                     /locus_tag="ACG8KR_002642"
     CDS             complement(51..1199)
                     /gene="nagA"
                     /locus_tag="ACG8KR_002642"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HFQ0201955.1"
                     /translation="MRTFIYADKFFLASGVKHAGYLEITDGLFGEYTKELPEGEVNII
                     DQSGKWIAPGLVDTHIHGYMNHDVMDNDAEGIKVMSEGLLSCGVTSFLPTTLTSSKER
                     LKDVAETIGKMYQEAPGAKIKGIYFEGPFFTEEHKGAQNPSYFGDPDLDTFHEWQEAS
                     GGLIKKIALAPERQGVEEFVKQVTEEGVVVSLGHSNATLEEAQKAVEAGASVFVHAFN
                     GMRGLNHREPGMVGALLSLEHVFSELICDGHHVHPNAAGILMEKAGHDHVALITDCMM
                     AGGMPDGNYNLGEFPVVVKEGTARLESGNLAGSILKLKEAIKNVVDWGLATPEQAVMM
                     ASLIPAISCKIDDTCGKIAQGRDADFIVLNPDMTLEATYLDGVERFHA"
     gene            complement(1275..2111)
                     /locus_tag="ACG8KR_002643"
     CDS             complement(1275..2111)
                     /locus_tag="ACG8KR_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0201956.1"
                     /translation="METLIESFGTIIKEIRKEQKMTQKMLSHDICSQSVLSRIENNEE
                     LPNVLVMMQICQRLGVTIDHVMNLSRKTTRTNDKLFELLDSCFQKRDYRKLEQVLKSS
                     DISENLYQATDFQRYYYYLGVCEFTLRQNISQSLYYLKEALSYSSQKERIHVSDTEIQ
                     LISCIGKIYGVAGKTAEARYYLSRSIQLLHQSADERSKSEFSQIFYNYGNFLFHQNEI
                     DEALEQVNQGIYWAQEKNSYYYLNDLFVLKSLIYKRKEEPERAAFYEGLAQAVKQISK
                     NL"
     gene            complement(2295..3512)
                     /locus_tag="ACG8KR_002644"
     CDS             complement(2295..3512)
                     /locus_tag="ACG8KR_002644"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFQ0201957.1"
                     /translation="MREFEKSNKLEGVSYDVRGPVLEEAERMQEEGISILKLNTGNPA
                     PFGFEAPNEVIRDLIMNARDSEGYSDSKGIFSARKAIEQYCQLKHFPNVTINDIYTGN
                     GVSELITMCMQGLLDNGDEVLVPMPDYPLWTASIALAGGTPVHYICDEEAEWYPDIDD
                     IKSKITSRTKAIVIINPNNPTGALYPKELLEEIVEVARQNNLIIYSDEIYDRLVMDGL
                     EHIPIATLAPDLFVVTLNGLSKSHRVAGFRVGWMVLSGDKSHVKGYIEGLNMLSSMRL
                     CSNVLSQQIIQTALGGYQSVDELLLPGGRIYEQREYIYNAINDIPGLSAVKPKAAFYI
                     FPKIDTKRFNILDDEKFVLDFLHEHHILLVHGGGFNWNQPDHFRIVYLPKMDDLKQTA
                     KKMREFLATYRQK"
     gene            complement(3661..3822)
                     /locus_tag="ACG8KR_002645"
     CDS             complement(3661..3822)
                     /locus_tag="ACG8KR_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0201958.1"
                     /translation="MEKYDGEFSGLGMILGVLIGLAFGRFLLGFMLGIICGIAMDWAA
                     NLWNDYHDN"
     gene            3895..3978
                     /locus_tag="ACG8KR_002646"
     tRNA            3895..3978
                     /locus_tag="ACG8KR_002646"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3929..3931,aa:Leu,seq:gag)
     gene            complement(4218..>4805)
                     /locus_tag="ACG8KR_002647"
     CDS             complement(4218..>4805)
                     /locus_tag="ACG8KR_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0201959.1"
                     /translation="FLDMNILFLLLFFLIFLLTYRKCQNYYVRILTALPFFLNLIIYF
                     GNTMGQSFTYVNGNKRAMIWSSSNLNNLFTELGTKLSLFYPGTWIATLVVLALLLCLI
                     VGIYLSFDNKKTSIFLVILMIMGFCSRLIMGFSPTVWASGMRTYYILYVVIAILVLMA
                     VKELMKSMSVQKNEFMQFGLTVLGICTFIITVINR"
BASE COUNT         1459 a         1001 c          775 g         1570 t
ORIGIN      
        1 ccttaaaacc aaacttaaga agtttagttt taaggttaac ttaaattata ttatgcgtgg
       61 aaacgttcca cgccatctag ataagttgct tccaatgtca tatcagggtt caatacgata
      121 aagtctgcat cgcgtccttg agcgattttt ccacatgtat catcgatttt acagctgatt
      181 gctggaatca gactagccat catgactgct tgttctggtg ttgctaatcc ccagtctaca
      241 acatttttaa ttgcttcttt caatttcaat atacttccag ctaagttgcc agattctaga
      301 cgtgctgttc cttcttttac tactactgga aattcgccta aattgtaatt tccatcaggc
      361 atcccaccgg ccatcataca gtctgtaatt aatgcgacat gatcatgtcc tgctttttcc
      421 attaggattc ccgcagcatt tggatgtaca tgatggccat cacaaatcaa ttcagaaaag
      481 acatgttcta aagataataa cgcacccacc atacctggtt cgcgatgatt caatccacgc
      541 ataccattga aagcatggac aaatacactt gcgcctgctt caacagcttt ttgcgcttct
      601 tctagtgtag cattactgtg tcctagagaa acaactactc cttcttcagt cacttgtttg
      661 acaaactctt ctacaccttg acgttccggt gctaaagcaa tttttttgat taaaccacct
      721 gaagcttctt gccattcatg gaaagtatcc aaatcaggat ccccgaaata acttgggttt
      781 tgtgcccctt tgtgttcttc tgtgaagaaa ggaccttcaa aatagattcc tttgatttta
      841 gctcctggtg cttcctgata cattttgccg attgtctcag ctacatcttt taaacgttct
      901 ttgcttgacg tcaatgttgt tggtaaaaat gaagtcactc cacaagataa cagaccttca
      961 gacatcactt tgatgccttc tgcatcatta tccattacat catgattcat atagccgtga
     1021 atatgtgtat ctaccaaacc aggagcaatc catttgccgc tttggtcaat gatgttgact
     1081 tctccctcag gtagttcttt ggtgtattca ccaaaaagtc catcggtaat ttcaagatag
     1141 cctgcatgtt tgacacctga cgctaaaaag aatttgtcag catagataaa agttctcatt
     1201 ctattctctc cctttgtttt tctacctcta aagtactctt atttagagaa gaagtcaaga
     1261 atggtctaca ccatttacaa attttttgaa atttgtttca cagcttgtgc taagccttca
     1321 tagaatgctg ctctttctgg ttcttcttta cgcttgtata ttaagctttt aagcacaaat
     1381 aaatcattga ggtaatagta gctattcttc tcttgagccc aatagattcc ttgattcacc
     1441 tgttctaacg cttcgtcgat ttcattttga tggaataaga aatttccata attataaaaa
     1501 atttgcgaaa attcactttt tgatcgttca tcagctgatt gatgcagcag ttggatgctt
     1561 cgactcaaat agtagcgtgc ttcagccgtt tttcctgcca caccatatat ttttccgata
     1621 caactaatca gttgtatttc tgtgtcagaa acatggattc tttctttttg gctagagtag
     1681 cttaatgctt ctttcaaata ataaagggac tgagagatat tttgacgcaa cgtgaattcg
     1741 caaacaccga gataataata atagcgttga aagtcagttg cttgatacag attttcagaa
     1801 atatcagaag attttagtac ttgttctaac ttccgataat cacgtttttg gaaacaacta
     1861 tccaacagtt caaataattt atcgtttgtc cgagtagttt ttcgagagag attcatcaca
     1921 tgatcaatcg ttacccctag ccgttgacaa atctgcatca tcaccaatac attcggcagt
     1981 tcctcattat tttcgatccg acttaataca ctttgtgaac aaatatcatg cgaaagcatt
     2041 ttttgcgtca ttttttgttc ttttcgaatc tctttaatga tcgttccaaa ggactcgatt
     2101 aacgtttcca taagtatcct ccccaaaata tgcgtattcg cattctattc gtattttact
     2161 ttaggaagtc gcaaacttca agaaaagact tgcaactaaa taatatactt tagttattta
     2221 agaaaactcc actagaaaga gagcctcagt gtgtttgtct gtggccctcc tactgaaaaa
     2281 tttggttttt aaaatcattt ttggcgatag gttgctagga attctctcat tttcttagct
     2341 gtttgtttca aatcatccat tttaggaaga taaacgattc gaaaatgatc tggctggttc
     2401 caattaaatc caccaccatg tacaagtaga atatgatgtt catgtaaaaa gtccaaaacg
     2461 aatttttcat catccaaaat attgaatcgc tttgtatcaa tttttgggaa tatataaaaa
     2521 gcagcttttg gttttacggc tgaaagacca ggaatatcat tgattgcatt atagatatat
     2581 tctctttgtt catagatcct tccgcctggc aggagcagct catcgacact ttgataaccg
     2641 cctagcgcgg tttggataat ttgctgagac aatacattgg aacacaaacg catggaggac
     2701 aacatattca gcccttctat atagcctttg acgtgtgact tatctccact taggaccatc
     2761 cagcctactc gaaatcctgc tacacgatga gatttggata gaccgttcag cgtaacaacg
     2821 aacaaatcag gcgctaatgt agcaatcgga atatgttcta gaccatccat cactaagcga
     2881 tcatagattt cgtcggaata aataatcagg ttattttgtc gagctacttc aacgatttct
     2941 tctaataatt cttttggata aagtgcccca gtaggattat taggattaat gataacaata
     3001 gcttttgtcc gactcgttat ttttgatttg atatcatcaa tatcaggata ccattctgcc
     3061 tcttcatcgc aaatataatg aacaggtgtg cctccagcta atgcaataga agccgtccac
     3121 aacggatagt ccggcattgg aaccagtact tcatctccat tgtccagcaa cccctgcata
     3181 cacatcgtga tcaattcgct cactccgttg ccggtgtaga tatcattgat cgtcacattc
     3241 ggaaaatgtt tcagttggca atattgctca attgcttttc tagcagaaaa gatccccttg
     3301 gaatctgagt agccttctga gtcacgggca ttcatgatca aatcacgaat gacttcgttt
     3361 ggtgcttcaa atccaaaagg tgcgggattt cccgtgttca attttaaaat actaatgccc
     3421 tcttcttgca tccgctccgc ttcttctaga acgggaccgc gaacatcgta actgacacct
     3481 tctaatttgt tggatttttc aaattctctc atcacttagc caatccctca tttcaaaaag
     3541 ttagaacttt ccaaattttt tgttatttac tatttcaata tcatactcca tcctagaaaa
     3601 ttttgccact tatttaggaa aaacgtaagt attaaaaaaa cacaaacaag cgttgagaac
     3661 ttaattgtca tgatagtcat tccatagatt cgctgcccaa tccattgcaa ttccacaaat
     3721 gattcctagc ataaaaccaa gcaaaaaacg tccgaatgca agtccaatca atacacctaa
     3781 gatcattccc agtcctgaaa attctccgtc atatttctcc atcttaataa gtcctcccat
     3841 ttttgagctt gacaactatg taaaacctcg ctatcattag tgtgttatta ttacgcagat
     3901 gtgttggaat tggcagacaa gcatgattga gggtcatgtg agcgttagct cgtgtgggtt
     3961 cgagtcccat catctgcact acaactcgtg aagttaattt tgcttcacga gtttttttgt
     4021 ttgaacgttt ctttatgcca ttatatacat atcactgtat cctttacaaa aagaacgtac
     4081 aatcatgtaa acagcctgag gtgagtatat gaaggtcgga aacaagctac tcgtagaaac
     4141 ttgcaagtgt taaaacaatt aatcgaaaaa cgagtaaaat ccctcaccta atactaaagc
     4201 gagggatttt ttgtatatta tctgtttatc accgttatta taaaggtaca aataccaagc
     4261 acggttaatc caaattgcat aaattcattt ttttgtacac tcatgctttt cattagctct
     4321 ttaactgcca ttagtactaa gatggcgata acaacataaa gtatatagta cgtccgcatt
     4381 ccggaagccc atactgtggg agaaaatccc ataattaacc ttgaacaaaa tcccattatc
     4441 atcaaaataa ctaaaaaaat agaagttttc ttattgtcaa aacttaaata aattccaaca
     4501 attaaacata gtaataatgc caagactact aaagtagcta tccaagtacc tggataaaaa
     4561 agtgacagtt tcgttcctaa ctctgtaaag agattattca ggttactact actccaaatc
     4621 attgcccttt tatttccatt gacgtaagta aagctttgac ccattgtatt accaaaataa
     4681 ataatcaggt ttaagaaaaa cggtaatgca gtcaatatac gcacataata gttttgacat
     4741 ttcctatatg ttagaaggaa aattaagaaa aatagcaata aaaataagat attcatgtct
     4801 aaaaa
//
LOCUS       DAZKLD010000167         4752 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.170, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000167 DAZKLD010000000
VERSION     DAZKLD010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4752)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4752)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4752
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.170"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(80..1888)
                     /gene="pepF"
                     /locus_tag="ACG8KR_002648"
     CDS             complement(80..1888)
                     /gene="pepF"
                     /locus_tag="ACG8KR_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HFQ0201960.1"
                     /translation="MEVKQLPKREELPENLTWDLTKIFSSDQEFDEKYLELSEELKQS
                     EKHKGTLDQGASQFLNAIEFVLRVYRQTEVIYVYAHLKNDQDTGNTDYQALYARASSL
                     FSKVSEAVSWFEPEILQLSDDQIWQYFKEEPKLEVYRHYIQQIVDNRAHVLSAEQESL
                     LAGAGEIFDASSDTFAVLNNADLVFPTIEGENGEIVQLSHGVYGQLLESTDRRVREAA
                     FKGLYSVYEQFRNTFASTLGTHIKGHNFKAKVRNYSSAREASLSNNHIPESVYDTLVD
                     VVNKHLPLLHRYMELRKRLLEVEKLHMYDLYTPVLGEAPITFTYEEAKEKALEALKPM
                     GEEYMAIVEKAFSERWIDVVENKGKRSGAYSSGSYDTNPYILLNWHDTLDQLFTLVHE
                     MGHSVHSYFTRSNQPYVYGDYSIFLAEIASTTNENILTEYLLETEKDPRVRAYVLNHY
                     LDGFKGTVFRQTQFAEFEHFMHTEDEKGVPLTSEYLSDSYGKLNAKYYGPAVEEDPEI
                     KFEWSRIPHFYYNYYVFQYSTGFSAASALAKKILNQEPEALENYLAYLKAGNSDYPVE
                     VMKKAGVDMTQAAYIEDAMSMFEQRLNELEELIDRL"
     gene            complement(2064..3062)
                     /locus_tag="ACG8KR_002649"
     CDS             complement(2064..3062)
                     /locus_tag="ACG8KR_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA family protein"
                     /protein_id="HFQ0201961.1"
                     /translation="MLLAMNEYNKCCFAKHAEKGKKYYCRGCRKRVILKKGKKKCAHF
                     AHQKSDNCSVFSERESKEHLQLKECFMDWLGQSVEPVFLEAYLPRLRQRPDILLANLA
                     IEIQCSRLSHQRFIERTQNYLNNSYQVWWILGNSFLGQSQFSLIEKSCCYYNRKRGVH
                     CWKADLKKQKLYLYHHITETVSGHISFCSSCWTFSSRDLKEIFTSNEIKMNQMKKIEK
                     VSEDGKKWLTRQLIHKQKNTVSIQEQCYLRHKHLLHLSSWIYQKSRFFFYLQEQVFLY
                     RMLYEEALENQKVPDFNSWLCQIKEHKREWLFPMIDEEMVYQQFFNECIHLSSLKK"
     gene            complement(3288..3941)
                     /locus_tag="ACG8KR_002650"
     CDS             complement(3288..3941)
                     /locus_tag="ACG8KR_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HFQ0201962.1"
                     /translation="MEMEHINENTIRVLIGHEDLEERGVSFLDLLGNHKEIENFFYSI
                     LEEVDIEEEFRGSEAVTFQVLPKGDGLELFISKNLPDEEISQSEDSTVDSEDVSDFLR
                     QQIIGSDPEEELSGSALEEQERQATFAFEDFEQVIQLATDVELESAWTDLYLFEERYY
                     LVVHFWMENLNQADVENQIARILEFAEKSDRTPETLNEYGQCLMARNAIERTKFYFV"
     gene            complement(4273..4671)
                     /gene="spxA"
                     /locus_tag="ACG8KR_002651"
     CDS             complement(4273..4671)
                     /gene="spxA"
                     /locus_tag="ACG8KR_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SpxA"
                     /protein_id="HFQ0201963.1"
                     /translation="MLTLYTSPSCTSCRKARAWLQEHQIPFVERNIFSEPLNSSELKA
                     ILQMTEDGTEEIISTRSKVFQKLNMDLDDLPLQELLELVQNNPGLLRRPIMIDDKRLQ
                     VGFNEDEIRRFLPRDVRQLELRQAQLMAGL"
BASE COUNT         1392 a         1001 c          743 g         1616 t
ORIGIN      
        1 aaaaaatacg tcgactccct tacaagagag ccgacgtatt tttttcgttt ctattcgaga
       61 aaatcaaaaa tagaaagcct tataaacgat cgatcaattc ttctaattca tttagacgtt
      121 gttcaaacat cgacattgca tcttcaatat atgcagcctg tgtcatatca actcctgctt
      181 ttttcattac ttccacagga taatcgctgt tacctgcttt taggtaagcc aagtagttct
      241 ctagtgcctc tggttcctgg tttagtattt tcttcgccag tgctgaagca gcagaaaagc
      301 cagtcgagta ttggaaaaca tagtaattat aataaaaatg cggtatccgt gaccattcga
      361 atttaatttc tgggtcttct tcgactgctg gaccataata ttttgcattc aatttaccat
      421 aactatcact taggtattca ctagtcaatg gcacaccttt ttcatcttcc gtatgcatga
      481 aatgctcgaa ttcagcgaat tgtgtctggc ggaaaactgt tcctttaaac ccatccaagt
      541 aatggttgag tacataagcc cgtacacgag ggtctttttc tgtttccaat aaatactccg
      601 ttaggatatt ttcattcgtt gtcgaagcaa tttctgctaa aaagatggag tagtcgccgt
      661 acacataagg ctggttcgaa cgagtgaaat aactatgaac actatgtccc atttcgtgga
      721 caagcgtaaa tagctgatcc agcgtatcat gccaattcaa taaaatatat ggatttgtgt
      781 catagcttcc cgaagaataa gcaccgcttc gtttcccttt attttcgaca acatcgatcc
      841 aacgttcaga gaatgctttt tctacgatgg ccatgtattc ttcacccatt ggtttcagtg
      901 cttctaaagc tttttctttt gcttcttcgt acgtaaaggt aattggagct tcccctaaga
      961 ccggtgtata aagatcatac atgtgcagtt tttccacttc taataaacgt ttccgtaatt
     1021 ccatgtatcg atgtaacaaa ggcaaatgct tgtttaccac gtctaccaaa gtatcgtata
     1081 cactttcagg aatatgatta ttgctcaaag acgcttctct ggcagagctg taattacgga
     1141 ctttcgcttt aaaattatgt ccttttatat gtgtgcctaa agtagaagca aatgtatttc
     1201 taaattgttc gtaaacactg tacaacccct taaatgctgc ttcgcgcacc cttcgatccg
     1261 tgctttctag caactgacca tacacgccat gagataattg gactatttca ccattttctc
     1321 cttcaatcgt tggaaaaact agatctgcat tattcaaaac agcaaatgta tcacttgaag
     1381 catcaaagat ttcacctgct ccagcaagaa gagattcctg ctcagcagat aagacatggg
     1441 ctcgattatc tactatttgc tggatataat ggcgatagac ttccaatttt ggttcttctt
     1501 tgaaatattg ccaaatctgg tcatctgaca attgcaatat ttctggttca aaccaggaaa
     1561 cggcttcact aactttcgaa aacagactgc ttgctcttgc ataaagcgct tggtaatctg
     1621 tatttccagt gtcttgatcg tttttaaggt gcgcatatac ataaatgact tcagtttggc
     1681 gataaaccct caatacgaat tcaatcgcat ttaaaaattg agaagcgcct tgatcaagtg
     1741 ttcctttgtg tttttcagat tgttttaact cttctgataa ttccaaatat ttctcatcaa
     1801 actcttggtc gcttgaaaag atcttggtca agtcccaagt taaattttca ggcaattctt
     1861 ctcgttttgg caactgcttt acttccatga aatccactcc ttttcttccc ttagctctat
     1921 ctatcaaaat agatcatagt ctaacatcca catgtcacag catctaactt ttctactcat
     1981 tcatgtgtct gcttccatca attttagcat aactaaatct tgttttacat agaaaaccga
     2041 ttcctagcct atttttatac ttactatttt ttcaaagaag acaagtgtat gcattcgtta
     2101 aaaaactgct gatagaccat ctcttcatca atcataggaa aaagccattc ccttttatgt
     2161 tcttttattt ggcaaagcca tgagttgaaa tcaggaactt tctggttttc caaggcttct
     2221 tcgtacaaca ttcgataaag aaaaacctgt tcttgtaaat aaaagaaaaa ccgacttttt
     2281 tgatagatcc acgatgatag atgcaaaaga tgtttatgac gcaagtagca ttgttcttgt
     2341 atggatactg tattcttttg cttgtgaata agttggcgtg tcagccattt cttcccatct
     2401 tcactcactt tttctatttt tttcatttgg ttcattttaa tttcgttact agtgaatatt
     2461 tctttaaggt ccctactaga aaaagtccag cagctactgc aaaaggaaat atgccctgat
     2521 acagtctctg taatatgatg atacaaataa agcttttgtt tcttgagatc agctttccag
     2581 caatgcactc ctctttttct attatagtag cagcaacttt tttctatcaa tgagaattgc
     2641 gattgtccaa gaaaagaatt accaagtatc caccatactt ggtaactgtt attcaaataa
     2701 ttttgggttc tttcaataaa tctctggtga gacagtctag aacattggat ttctattgct
     2761 agattcgcca acaaaatatc cggcctttgc cgcaacctcg gcaaataggc ttccaaaaat
     2821 actggttcta ctgactgtcc cagccaatcc atgaagcatt cctttaactg taagtgctct
     2881 tttgattctc tttcactaaa gaccgaacag ttatctgatt tttgatgggc aaaatgagca
     2941 cattttttct ttcccttttt caaaatcact ctttttcgac agccacgaca ataatatttc
     3001 tttccttttt ctgcatgttt agcaaaacag cacttattat attcattcat tgcaagcagc
     3061 atttttctcc ctctgaactt atttacgtct aagaatttat ttacacgaaa aaagagggcg
     3121 tgaaaaagct attctgcatc aagtaataag aacagctaaa caaaaatagt tcccaatatt
     3181 tttcgtttgg ttgggcttgt taccgcagat gcaagctttt gaacacattt atacgaaaaa
     3241 aagactgcga cacaattttt gtcacagtct cttactataa tgctgattta tacaaaataa
     3301 aatttcgttc gctcgatagc attacgcgcc atcagacatt gtccatattc attcaatgtt
     3361 tctggtgtac gatcgctctt ttcagcaaac tccaaaattc gagcaatctg attctctaca
     3421 tccgcttggt tgagattttc catccagaag tgaacgacta ggtaatagcg ctcttcgaat
     3481 aaatacaaat cagtccaagc agactctagt tcgacatctg ttgcaagttg gatcacttgt
     3541 tcaaaatctt caaaagcaaa agttgcttgt ctttcctgtt cttctagggc gcttccagat
     3601 aattcttcct ccggatcgct gccaataatc tgctgacgaa gaaagtcact cacatcttct
     3661 gaatcaacag tagaatcttc tgattgagaa atttcttcat ccggaagatt tttactgata
     3721 aacagttcta atccgtctcc ttttggcaaa acttggaaag tgacagcttc gcttcctcgg
     3781 aactcctctt cgatatcaac ttcttccaaa atactataaa agaaattttc gatttctttg
     3841 tgattgccaa gtaaatccaa aaatgaaacg ccccgttcct ctaaatcttc atgaccaatt
     3901 agtacacgga tcgtattttc attaatatgt tccatttcca ttttgctaca cctcactttg
     3961 ccacaatgtt taatatagat tcattgtagc gaaatcagct taaatgtaaa gaaaaaaaat
     4021 ctcgctgtta agcgaattta ataaaaccag cggatttttc aacctttcaa gctatcttat
     4081 ttaactataa catttttggc agacgaatac tatatagccg acttttcagc taattttccc
     4141 tctctatttt tcgtgcaatc atcatcttct actacctttt tatacatcta atcaagaata
     4201 ctttttgtaa aaacaaaaaa acttggaaaa actatctttg taatagtctt ttccaagttt
     4261 cgtattatcc ttctataatc cagccatcaa ttgtgcttga cgtaattcta attgtcgtac
     4321 atcgcgcggt aggaaccgac gaatttcatc ttcattaaaa ccaacttgca gacgtttatc
     4381 atcaatcatg attggacggc gaagcaatcc aggattgttt tgcacaagtt ccaataattc
     4441 ttgtaacggc agatcatcta aatccatgtt cagtttttgg aatactttgg aacgtgtcga
     4501 gatgatttct tctgttccgt cttctgtcat ttgtaaaatc gcttttaatt cagaactgtt
     4561 caatggttct gaaaaaatat tacgttcaac aaatggaatc tgatgttctt gcaaccacgc
     4621 acgagccttt ctgcaggaag tacaacttgg ggaagtatat aatgttaaca acgtgcacca
     4681 ctccttttcg tgataagaat cacttttttg taacagaaga tcataataat ctcccatctg
     4741 tttctattat ac
//
LOCUS       DAZKLD010000168         4737 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.171, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000168 DAZKLD010000000
VERSION     DAZKLD010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4737)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4737)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4737
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.171"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(201..1163)
                     /locus_tag="ACG8KR_002652"
     CDS             complement(201..1163)
                     /locus_tag="ACG8KR_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004233175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0201964.1"
                     /translation="MVAKLTDVAELAGVSPTTVSRVINNKGYLSEKTKQKVHEAMKTL
                     GYKPNNLARGLQGKSPQLIGLIFPNISNIFYSELIEYLEIELFKHGYKTIICNSQNDP
                     AKEREYIEMLEANQVDGIISSSHNLGIADYERVRAPIVAFDRNLAPNVSIVSSDNFEG
                     GKLAAKTLQKNGCQNTIMITGNDNTDSPTELRALGFSFQNPNGKIFKIPNNLSTIRRE
                     MEIKSTIINHKPDGIFVSDDLTAILTMKIAHQLKLNIPEDLKIIGYDGTSFIEKFFPQ
                     LTTIRQPIDEIASLIVDILLKKIKGEKTNKDYILPISILSGGSI"
     gene            complement(1165..2604)
                     /locus_tag="ACG8KR_002653"
     CDS             complement(1165..2604)
                     /locus_tag="ACG8KR_002653"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004564 - beta-fructofuranosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HFQ0201965.1"
                     /translation="MNLPREIRYRAYSDWTKDEMDKIKDNVKRSPWRASYHIEPKTGL
                     LNDPNGFSFFNGKYTLFYQNWPFGAAHGLKEWVHNESDDLVHFHVTGAELLPDTKRDS
                     HGAYSGSAYEIEGNLFLLYTGNVRNENWERDTLQIGAWMDKDYNITKGENVLIHKPAD
                     VTDHFRDPQMFNYEGQLYAIVGAQNLEKSGFIKLYKADDNNVETWEEVGNLDFGGTGS
                     EYMIECPNLVFVDKKPVLIYCPQGLDKSELNYGNIYPNTFKICQSLDIEKVKLVGASE
                     IQNLDYGFEAYATQGFNTPDGRALIVSWIGLPDIDYPTDKYDYQGAMSLVKELSIKDG
                     KLYQYPVEAITSLRASSEQFTAKAETNNTYELELTFPPNQKSELLLFSDSKGNGLSLI
                     VDTKEGKIILDRSKAGIQYATDFGTIRECSIDTKETTANIFVDNSIIEIFINKGEKVF
                     TSRVFPENHQNGIQIKSGEPTGKYFELKY"
     gene            2789..4735
                     /locus_tag="ACG8KR_002654"
     CDS             2789..4735
                     /locus_tag="ACG8KR_002654"
                     /EC_number="2.7.1.211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-specific PTS transporter subunit IIBC"
                     /protein_id="HFQ0201966.1"
                     /translation="MNYSKVASDVIQAVGKDNLIAAAHCATRLRLVLKDDTKVNQKAL
                     DENPDVKSTFKIDGQYQVIIGAGDVNFVYDELIKKTGLSEISTDDLKQIASKDKKFNP
                     IMALIKLLSDIFVPIIPALVAGGLLMALQNFLTAAGLFGPKSIEEMYPAIEGISSMIQ
                     LMSAAPFMFLPILVGISAAKRFGANQFLGAAIGMIMTTPDLGGSTEYWNIFGYHVSQT
                     NYAYQVIPVLAAVWILSILEKFFHKKLPSSIDFTFTPLLSVMITGFLTFTVIGPVMLL
                     LSNGITDIIVWLYNTTGFIGMGIFGGTYSLIVMTGLHQSFPAIETQLLSAWTNGIGHG
                     DFIFVVASMANVAQGAATFAIWFLTKNSKTKSLASSAGVSALLGITEPALFGVNLKYR
                     FPFFCALIGSGIATAVAGLLKVVAVSLGSAGFLGFLSINATSIPFYVLCELISFVVAF
                     AITYFYGKTKAAGIFKAEEIAEQTPVNSSKVATTDPISTDVESTQTKVSSETIVSPIS
                     GKVISLSSVNDPVFSSEAMGKGIAIKPNGNTVHSPVDGTIQLTFETGHAYGIKSNNGA
                     EVLIHIGIDTVSMGGKGFTQKVTTNQKVRKGDILGAFDRSMIEKEGLDDTTMIIVTNT
                     SDYSEVNIVAENTVTEGTDLLEIK"
BASE COUNT         1506 a          811 c          784 g         1636 t
ORIGIN      
        1 atttcgtaaa acaaccgtaa cagttgtgta aaaatatcat tttagagcat gaaaaaagga
       61 gtggatttct ccactccaaa aaagtctaac ttttgggggt cactacaagt tgacaactcc
      121 tttttgttta caaatcaatt ggatatctac aaattgtatt ttctaacgaa ttttatagac
      181 attaaaaaat taatttaacg ttaaatactt cctccagaaa gtattgagat tggtaaaata
      241 taatccttat ttgtcttttc gcctttaatt tttttcagaa gaatatcaac aataagactt
      301 gcaatttcat caattggttg tcttattgtt gtcaattgtg ggaaaaattt ctcaatgaaa
      361 gaggtaccat cgtatccaat aatttttaaa tcttctggga tattaagttt aagttgatga
      421 gcaattttca tagtgagaat ggctgttaaa tcatcagaaa caaaaatacc atctggtttg
      481 tgatttataa tagttgactt tatttccatt tcacgacgga tagtagacag attattagga
      541 attttgaaaa tttttccatt tggattttga aaagaaaaac ctagtgcacg taattctgtg
      601 ggagaatccg tgttatcatt tcctgtaatc ataatggtat tttgacaacc atttttttgt
      661 aaagtcttag ctgcaagttt tcccccttca aagttatctg aagaaacaat ggaaacatta
      721 ggagctaaat ttctatcaaa agcaacaata ggtgctctca cacgctcata atcagcaatt
      781 ccaagattat ggcttgatga gattatgcca tcaacttggt tggcttcaag catttcaatg
      841 tattctctct ctttagcagg atcattttga ctattgcaaa taatcgtttt atatccatgt
      901 ttaaataact ctatttcgag atactcaatg agttcagagt aaaagatatt actaatatta
      961 ggaaaaataa gaccaataag ctgaggtgac tttccttgaa gtccacgtgc taaattattt
     1021 ggtttataac caagagtttt catagcttca tgaacttttt gttttgtttt ttctgataaa
     1081 tatcccttat tattaatgac gcgtgaaacg gttgttgggc taactccagc taattcagcg
     1141 acatcagtta attttgctac cattttaata ttttaattca aagtattttc cagtaggttc
     1201 gcctgatttg atttgaatac cattttggtg attttcaggg aatacacgac tagtaaacac
     1261 tttttctcct ttattaataa aaatctcaat gattgagtta tctacaaaaa tgttagcagt
     1321 tgtttcttta gtatcaattg agcattcacg aatcgtacca aagtcagtgg catattgaat
     1381 gcctgctttg ctgcgatcta aaataatttt tccttctttg gtatctacaa tcaagctaag
     1441 tccatttcct ttactatcag agaaaagaag taactccgat ttctgatttg gaggaaaagt
     1501 tagttcaagc tcataagtat tatttgtttc tgcttttgct gtaaattgtt cagaactagc
     1561 acgtaatgat gtaatagctt ctactggata ttggtaaagt ttaccatctt ttattgaaag
     1621 ttcttttaca agactcattg ctccctgata atcatactta tcagttggat aatcgatatc
     1681 aggtagtcca atccaactga caatcagagc acgaccatca ggagtattga atccttgagt
     1741 cgcatacgct tcaaatccat aatcaagatt ttgaatttct gaagcgccca caagttttac
     1801 tttttctatg tcaagcgatt gacagatctt aaaagtattc ggatagatat tcccataatt
     1861 tagctcagat ttatccaacc cttgaggaca ataaatgaga acaggttttt tgtctacaaa
     1921 tactagattt ggacactcaa tcatgtattc tgaaccagtg ccgccaaaat caaggttgcc
     1981 aacttcctcc catgtttcca cgttattgtc atccgctttg taaagtttga taaagcctga
     2041 tttttctaaa ttttgtgctc caacaatcgc atacaattgt ccttcataat taaacatttg
     2101 tggatcacga aaatgatcag ttacatcagc aggtttatga ataagaacat tctcaccttt
     2161 cgtaatgttg tagtctttat ccatccatgc tccgatttga agtgtatcac gttcccaatt
     2221 ttcatttcga acgtttcctg tatagagtaa gaatagattc ccctctattt catatgcaga
     2281 accggaataa gcgccgtggc tatctcgttt tgtgtctgga agaagttctg ctcctgttac
     2341 atgaaaatga actaaatcat ctgattcatt gtgtacccat tctttcaacc catgagcagc
     2401 tccaaatggc caattttgat aaaaaagcgt atattttccg ttaaaaaaag aaaaaccatt
     2461 aggatcgttt aacaatcctg ttttaggttc gatatgatag ctagcacgcc aaggtgatcg
     2521 ttttacatta tccttaattt tatccatctc atctttagtc caatcagaat aagcacgata
     2581 tcgtatttct cgaggtaagt tcatatttta ctccttcaat ttatcttttt acaactagta
     2641 tggtaaacgt tttccgttga aaaatcaatc atttcattta aaaaaataaa ctatatgaca
     2701 aacgtttgac atatttttaa gatatgctaa aataaatttg catttcagtg aaccgttttc
     2761 acgtaaaaat aaaaaaggag tgattcgaat gaattacagt aaagtagctt ccgacgtaat
     2821 acaagcggtc ggaaaagata accttattgc agctgcacac tgcgctacac gtcttcgtct
     2881 cgtactaaaa gatgacacga aagtgaatca aaaagcttta gacgaaaatc cagatgtgaa
     2941 aagcacattt aaaattgatg gacaatatca agtgattatc ggtgcaggtg atgttaattt
     3001 cgtttatgac gaacttatca aaaaaactgg actttcggaa atttcaacag atgatttaaa
     3061 acaaattgct agtaaagata aaaaattcaa ccccattatg gcattaatta aattattatc
     3121 agatattttt gttccaatta tacctgccct tgttgcaggt ggtttattaa tggcattaca
     3181 aaacttttta acagctgctg gactttttgg accaaaatca attgaagaaa tgtatcctgc
     3241 gattgaaggt atttcctcaa tgatccaact gatgtccgct gcaccattca tgttcttacc
     3301 aattttagta ggtatttcag ctgctaaacg ttttggagca aaccaatttc ttggtgctgc
     3361 aattggtatg atcatgacaa caccagatct tggtggctca actgaatatt ggaatatttt
     3421 tggttatcat gtttcacaaa caaactatgc ttatcaagtt attccagttt tagcagctgt
     3481 ttggatatta tctatattag aaaaattctt tcataaaaaa ttgccatcat ctattgactt
     3541 tacattcacg ccattattat cagtaatgat tactggattt ttgactttca cagttattgg
     3601 ccctgtaatg ttattgttgt ctaatggaat tacagacatt attgtttggc tatataacac
     3661 tactggattt attggaatgg gtatttttgg aggaacatat tcacttatag tcatgactgg
     3721 tttacatcaa tcatttccag ctattgaaac tcaattactt tcagcgtgga caaatggtat
     3781 cggacatgga gactttatct ttgtagttgc atctatggca aacgttgctc aaggtgctgc
     3841 aacatttgcc atttggttct taactaaaaa ttctaagaca aaaagtcttg cttcctctgc
     3901 gggtgtctca gcacttcttg gaattacaga gcctgccttg ttcggagtta acttaaaata
     3961 ccgctttcca ttcttttgtg ctttaattgg ttcaggaatt gctacagcag tagcaggatt
     4021 acttaaagta gttgctgttt cacttggttc agcaggtttc ttgggatttc tttcaattaa
     4081 tgcaacatct attccatttt acgtattgtg tgaattgatt agctttgtag ttgcttttgc
     4141 aattacttac ttctacggta aaacaaaagc tgctggtatc tttaaagcag aagaaattgc
     4201 tgaacaaaca ccagttaatt cttcaaaagt agcaacaaca gatccaatat ctactgatgt
     4261 tgaatcaaca caaacaaaag tttcatcaga aacaattgtt agtcccattt ctggtaaagt
     4321 gattagtctt tcttcagtaa atgatccagt cttttcatca gaagctatgg gaaaagggat
     4381 tgccattaaa ccaaatggaa acacggtcca ttctccagta gatggtacta ttcaactcac
     4441 gtttgaaact ggacatgctt atggtattaa atcaaataat ggtgctgaag ttctaattca
     4501 tattggtatt gatactgtat caatgggagg aaaaggattt acacaaaaag ttactactaa
     4561 tcaaaaagtt agaaaaggcg atattcttgg agcatttgac cgttcaatga ttgagaaaga
     4621 aggtcttgat gatacaacaa tgattattgt gaccaatact tcggattatt cagaagtaaa
     4681 tatagtagct gaaaatacag tcactgaggg tactgattta cttgaaatca aataaat
//
LOCUS       DAZKLD010000169         4719 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.172, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000169 DAZKLD010000000
VERSION     DAZKLD010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4719)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4719)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4719
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.172"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            115..561
                     /gene="nrdI"
                     /locus_tag="ACG8KR_002655"
     CDS             115..561
                     /gene="nrdI"
                     /locus_tag="ACG8KR_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HFQ0201967.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEYEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQRFGFPFLADYELRGTPSDVTRVYQILAEKNK"
     gene            complement(631..2613)
                     /locus_tag="ACG8KR_002656"
     CDS             complement(631..2613)
                     /locus_tag="ACG8KR_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose PTS transporter subunit IIA"
                     /protein_id="HFQ0201968.1"
                     /translation="MAKNELTLEELAKQIYQGAGGMGNVESIVHCMTRVRMKVINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQEMVDLAGVKLGEKLPSASATTAS
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAGTWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALATGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKAVPQASA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVISSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAADK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(2627..3523)
                     /gene="murQ"
                     /locus_tag="ACG8KR_002657"
     CDS             complement(2627..3523)
                     /gene="murQ"
                     /locus_tag="ACG8KR_002657"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA];
                     GO_process: GO:0046348 - amino sugar catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HFQ0201969.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELPSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISEHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEAAQAKLAEANGFVKGTL"
     gene            complement(3526..4587)
                     /locus_tag="ACG8KR_002658"
     CDS             complement(3526..4587)
                     /locus_tag="ACG8KR_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MupG family TIM beta-alpha barrel fold protein"
                     /protein_id="HFQ0201970.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQNRLIELGSEAKKNKLELMVDISGEALKRAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPNDIDELKEAAADFSQIEAWHNYYPRLETGLDKEWYQEK
                     NQWLKTQGFLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSEHVQEQFASFQKEQTVLLHTEPVDEVFYEYILGKHTNRQDDARDVIRSADARF
                     REIPPIPVRNTAKRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
BASE COUNT         1289 a         1058 c          845 g         1527 t
ORIGIN      
        1 tctaccgact tttttctcat tatacaagca aagcgcctag tccctttcca aattacacca
       61 ccaaaaaaac ttaaaaagat gctatactaa agggaaagaa ttgggggatg atccatgaac
      121 attttatata tatcgatttc aggaaatacg cgtgcttttg ccaaacatct agcagaatat
      181 gctgaaaaaa tgcatgcaga agatcctgtc aatcctgaag ttactttaaa agaaatccat
      241 gaaaatagtg attttacgaa agaaacagaa ccatttttta ctttcgttcc tacttattta
      301 gacggcggga atgggttaga caacggtgac acagaaatct tgaccgaaac gatgcgcgaa
      361 tatctggaat acgaagacaa tcataaactt tgccttggtg tagtcggtag cggtaataaa
      421 aacttcaaca atcaatattg tctgacagcc aaacaatacg cacaaagatt cggctttcct
      481 ttcttggcag attatgaatt acggggaaca ccatctgatg tgacacgcgt ttatcaaatt
      541 ttggcagaaa aaaataaata acacaaaaga agaagctgaa aaataaattt cggcttcttc
      601 tttttatgtt ctctattgtt tctattctta ttaagctttg acatccatca caggtgcttt
      661 tgctttcgct ttacctgttt tttccaagac aaagtttttt acacgatcca tattggttac
      721 caagacgatc atggaggttt ctttgtctgc tgctttgatt gcttctaagt cagcttctgc
      781 tacaagcgta tcaggtgtca cttgctgtcc ttctgttact ttcacatcga atggcgtacc
      841 gcctaagtca actgtgttga tgcccatgtg aagcaagatt tctaagccat ttggtgtcgt
      901 aatgccgata gcatgttttg ttgggaaaac agaacttatt actccagtaa ccggggaata
      961 gatatgtccg ttttctggtt ctacaaagta accttcaccc atcatttttt gtgcaaaaac
     1021 tgggtcactt gctttatcta aagaaacaac tgttccgtca gcaactgatg ataagatagt
     1081 cacactttca gctgaagctt gaggtactgc tttttcaggt acttcattta ctgtggctgt
     1141 ttcggtcgct agttcttctt tttctaaacg gtctttagga ataccgaaga agaatgtagc
     1201 aacgaagcca ccagcgtaag cagccaataa accaaggata tagcctaacc atttaccatc
     1261 agagatcaat ggaatcaaag cggctcctga aggtccaata gcgattgctc cgacatttcc
     1321 gatcattccg atcactgccc caccgatacc tccaccgata caagcagtaa taaatggacg
     1381 tcctagaggt aatgttactc cgtaaatcaa tggttcgccg attcctaaga ttccgactgg
     1441 taaagcccct ttgatcattt ctgttaattc tttgtctttt ttgcagcgga tccataatgc
     1501 caatgctgca cccacttgtc ctgctccggc catcgcaagg attggaagca ataaggtacg
     1561 accagtttca tcaatcattt gtacatggat tggcgtcaag atctgatgta gtccgaacat
     1621 gaccattggc aagaataatg tacctagaac gaagccggca aatgctcctc caacaccaag
     1681 tacccagttg atcccaccga ccatgccgtc agaaatgaac cctgctaaag gcatgatgaa
     1741 gaaaatttcg atcagaccga tggcaatcag ggaaagagtt ggcgtaacga tgatatctac
     1801 cgcatcaggt actactttat gcagtttttt ctcaacgatt gacaataacc aaactgcgaa
     1861 gataacacct aagatcccgc cttgtccagt tgcaagagct tcaccggtaa acaagttttt
     1921 cagtggtgct tctggattca tccctgtcag catcacaaca gccccgataa ctccaccaag
     1981 cgttggcgtt gcaccaaaaa cttgtgcagc attgatacct gtatagataa ctagatagct
     2041 aaacatcccg tttttcaaga tgttcatcac atcgatatat tgttgccatg ttcctgcgtc
     2101 tagtgttcca gctgtgacta agtttgctaa aactgcggca atccctgcca cgatcccagt
     2161 gccgacaaat gcagggatca ttggtacaaa gatattagag atgtctttta ataccgattt
     2221 gattttagag ggtttttgtt tcgatttttg tgccgctttc atttcttgtg cccgttcgtt
     2281 tacacggtct tttccagatg cagaagaact tgctgtagtt gcactagcag aaggaagttt
     2341 ttctcctaat ttcacgcctg ctaagtcaac catttcctgc gctactttgt tcactttccc
     2401 aggtccgatg atgacttgga gctgttcatc ctctactacg ccaagtactc ctggaatcgc
     2461 ttttaattcc gagacattta caagatcatc attgatgacc ttcatacgta cacgagtcat
     2521 gcagtggaca atgctttcaa cattgcccat accgcctgct ccttgataaa tttgttttgc
     2581 cagttcttcc aaagtcaatt cgttttttgc catggtcttt tccccctcat aaggttcctt
     2641 ttacaaaacc gtttgcttca gctaattttg cttgagctgc ctctttatca ctatttgtca
     2701 aaatcatgac gatagccagt ttgacatttt gatctgcttc ctcaaagtac ttttcagctg
     2761 tttcgtagtc agcttctgtc gcttgcatga tgattcgttt tgagcgttca attagttttt
     2821 catttgttgg tttgacgtcg accatcaagt tattgaatac tttaccgata ccgatcatac
     2881 tgatagtaga aatcatattc aaaatcagtt tttgtgctgt tcctgacttc aatcgagtag
     2941 agcctgtcaa aaattctggt cctgcatcga cttcgattgg gaaatccgca tgctcgctga
     3001 tttctgcttg tttattgcaa gaaatcgttc ctgtaacagc accaatcgat tttgcgtaat
     3061 ctaatccacc aatcacatat ggtgtacgac cgctagctgc gataccaata actatatcat
     3121 tttttgttaa attcagcttg attagatctt cttgccctaa ttcataagaa tcttcggctc
     3181 cttcgacggc tacagtcatt gcttcttctc caccagcaat taggcctatg accatttccg
     3241 gttctacacc gaatgtcggt acacattcag cagcatctaa tacaccaagt ctaccactcg
     3301 ttccagctcc catataaatc aagcgaccac cttttttaaa ggattcaatc gtgttggcga
     3361 tcactggctc gatacttggc agttcttttt caacagcagc ggccactttc tgatcttctt
     3421 tgttcatgag ttggactgct tgagaaatac tcatttcatc taatccaaaa gtttcttggt
     3481 ttctgcgttc cgttgttagg ttctctaaat tgattttgct catttttatc ttccttcttt
     3541 tcttataaat tgatagttca taccagcttt gatttggttg atcagcggta gttcttcttt
     3601 gatgactttg gctactacat tcactttttc atccgctggc aaatcatatt tagttaattg
     3661 gatctcaccc atatagcgca aatacttttc gttatctaaa gtaatagatc ctttcatacg
     3721 cttagctgta tttcttactg ggattggcgg aatctctcga aaacgcgcat ctgcgcttcg
     3781 tatgacatct cttgcatcat cttgccgatt cgtatgtttt cctaatatat attcgtaaaa
     3841 aacttcatct acaggttccg tatggagcaa gactgtctgt tctttttgga aagaagcaaa
     3901 ttgctcctga acgtgttctg acagacctgc atctccgata taaacaatat ccgtgttact
     3961 tgctaataga tcaagagcac ctgccagagg atggatccct cgatgttctt ccaaagttgg
     4021 taatccttga tacagtggac ctctcaactc tgtatctcct ggtacaaagc cttgtacaag
     4081 gaatccttga gttttgagcc attgattttt ttcctgatac cattctttgt ccaaccctgt
     4141 ttcaagacgt ggataataat tatgccaagc ttcaatctgt gagaaatcag ccgctgcttc
     4201 tttcaactca tcgatatcat taggcgtgat cgtgctggca ttcaaactga ttttcaattg
     4261 atgtgaccag tcggcgatct gctgattaga aatatgataa tccattctta accctgttac
     4321 accaattgct ttcaagggtg ttagatcttc taatgaaaag ccagctcgtt tcaatgcctc
     4381 tcctgaaata tcgaccatca actctagttt attctttttc gcttcgcttc ctagttcgat
     4441 caatcgattt tgataagccg aaacatcatc ttccggtata tgcatggaag tgaaaatacc
     4501 gacaaagcca ttggatgcca tttggcggat atactgtttc ttctcttctg acaaagaatc
     4561 attcataaat attgaaaagc caaacatatt atccctcttt ttctgtatcc gtcttcattt
     4621 ctatgactag agtatatatc taaagaaaca aaatttcaat acttttgtta aaataattaa
     4681 attttatttc taaaaattgg atttataatg ccaaaagaa
//
LOCUS       DAZKLD010000170         4702 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.173, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000170 DAZKLD010000000
VERSION     DAZKLD010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4702)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4702)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4702
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.173"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(81..368)
                     /locus_tag="ACG8KR_002659"
     CDS             complement(81..368)
                     /locus_tag="ACG8KR_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5960 family protein"
                     /protein_id="HFQ0201971.1"
                     /translation="MNKEYFVQQDAFLEDYKSASKKLTINIDELADQVLSQLNETQEE
                     YYTMPANDAKDNVSHRFPFSRRIYSDENDGTLNTEYTYEGQPYVLETDDDL"
     gene            complement(539..1891)
                     /locus_tag="ACG8KR_002660"
     CDS             complement(539..1891)
                     /locus_tag="ACG8KR_002660"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidase"
                     /protein_id="HFQ0201972.1"
                     /translation="MKVVVIGCTHAGTAAVKSILKNHPEAEVTVYERNDNISFLSCGI
                     ALYVGGVVKDPAGLFYSNPEELSSLGATVKMEHDVTDVDTENKTVTAKDLKTGETETV
                     SYDKLVMTTGSWPIIPPISGIDAKNVLLCKNYNQANEIIAQAKEAKRVVIVGGGYIGI
                     ELVEAFVESGKQVTLIDGLDRILNKYLDKPFTDILEKELTDRGVTLALGENVTEFITD
                     EEGKVKQVATPTDTFDTDMVILCVGFRPNTKLIEGKVDTLPNGAIKVNEYMQTSDPDI
                     FAAGDSAVVNYNPSGTQNYIPLATNAVRQGLLVGNNLTDHKMAYRGTQGTSGLYLFGW
                     TIGSTGVTKESATLNDLEVQATVFEDNYRPEFMPTTEKVMMELVYEKGTNRIVGAQFM
                     SKYDITQSANTMSLAVQNKMTVEDLALSDFFFQPHFDRPWNYLNLLAQEALGEIETAQ
                     "
     gene            complement(2163..2609)
                     /locus_tag="ACG8KR_002661"
     CDS             complement(2163..2609)
                     /locus_tag="ACG8KR_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HFQ0201973.1"
                     /translation="MENKLVEEVIEKLKKENIRITPQRYAVLEYLIEHHSHPTADEIY
                     KALEHRFPNMSVATVYNNLRLFTSIGFVQEMKYGDASSRFDFSSKRHYHVICENCGKI
                     VDFHYPGLEDIEMAASRLTDFEINEHRLELYGLCPDCKESAKKAQM"
     gene            complement(2845..3771)
                     /gene="cysK"
                     /locus_tag="ACG8KR_002662"
     CDS             complement(2845..3771)
                     /gene="cysK"
                     /locus_tag="ACG8KR_002662"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HFQ0201974.1"
                     /translation="MTAIYNSVTELIGKTPIVKLNKIVPEDSADVFVKLEFFNPGGSV
                     KDRIALSMIEKAEHDGLLKPGDTIIEPTSGNTGIGLSMVGVAKGYKVIIVMPETMSIE
                     RRLLMKGYGAELILTPGADGISGSIREAERLAKENGYFLPLQFENEANPLVHEKTTGP
                     EIHQAFGVNGLDAFVAGIGTGGTITGAGRELKRVYPKIELIGVEPAESAILEGKEAGP
                     HKIQGIGTGFVPKTLDTSVYDKVLSISGDEAMETAREVGRKEGILVGISSGAAIAAAL
                     KVAKELGKGKKVLAVVPDNGERYLSTALYQEI"
     gene            complement(3911..4702)
                     /locus_tag="ACG8KR_002663"
                     /pseudo
     CDS             complement(3911..4702)
                     /locus_tag="ACG8KR_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627954.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
BASE COUNT         1320 a          988 c          783 g         1611 t
ORIGIN      
        1 atagtaaaga gacagtcatc ggtaattgtg atgatttttt gtcatcactt aaggatgagc
       61 ggccttttca aaaactgtct ttaaagatca tcatcagttt ctagcacata tggctgacct
      121 tcatatgtgt actctgtatt caatgtgccg tcattttcat ctgagtagat gcgtctactg
      181 aaaggaaaac gatgagaaac attatctttg gcgtcatttg ctggcatcgt gtaatattcc
      241 tcttgcgttt cattcaactg agagagtact tgatctgcca actcatcaat attgatcgtt
      301 aatttcttcg aagcactttt ataatcttct aaaaatgcat cttgttggac aaaatattct
      361 ttattcatct tctttctcac ctttcttttc aaaatcaaat aaagctgctt gttcttttat
      421 gtaacagctt tatctttgct gcttattgtg acaaaaaatt cgaagaaaga caactaattg
      481 tacttttcac aaaaaaagac agagggtcgt gacaaaattg tgtcacaacc ctttttcttt
      541 attgtgcagt ttcgatttcg cctagcgctt cttgtgcaag taggttcaag taattccatg
      601 ggcggtcaaa atgtggttgg aagaagaaat ctgaaagtgc gagatcttct acagtcattt
      661 tattttgaac agctaaagac atggtatttg ctgattgtgt aatgtcgtat ttggacatga
      721 actgagcacc tacgatacga ttcgttcctt tttcatagac tagctccatc attacttttt
      781 cagtagttgg catgaactct ggacgataat tatcttcaaa tacagtcgct tggacttcta
      841 gatcgttcaa agttgcgctt tctttggtca cacctgtcga accgatcgtc cagccaaata
      901 ggtaaagtcc tgaagttcct tgagtaccac gatacgccat tttatgatct gtcaagttat
      961 ttcccacaag taaaccttga cgaacggcat ttgttgccaa aggaatatag ttttgcgtgc
     1021 cacttggatt gtagttgact actgcagagt ctccagctgc aaaaatgtct ggatcacttg
     1081 tctgcatgta ttcattcact ttgattgccc cgtttggcag tgtatctact tttccttcga
     1141 tcagttttgt atttggacgg aagcccacac ataaaatgac catatctgta tcaaatgtat
     1201 ctgttggtgt agctacttgt ttcacttttc cttcttcatc tgtgataaat tcagtaacat
     1261 tttcgcctag cgcaagagta acaccgcggt ctgtcaattc tttttctaag atatctgtga
     1321 atggtttatc tagatattta ttcaagatgc gatctaatcc atcaatcaat gtcacttgtt
     1381 ttccagattc gacaaaagct tctactaatt caataccgat atatccacca ccaacaatta
     1441 ctacacgttt tgcttctttc gcttgtgcaa taatttcatt tgcttgattg tagtttttgc
     1501 ataggagtac atttttcgca tcgattccac taatcggtgg gataataggc catgaaccag
     1561 ttgtcatcac taatttatca taagaaaccg tttctgtttc tcctgttttc agatcttttg
     1621 ccgttactgt tttattttct gtatcgacgt ctgttacatc gtgttccatt ttaactgttg
     1681 ctcctaatga agataattct tcaggatttg agtagaacaa gcctgctggg tcctttacta
     1741 ctccgccgac ataaagtgcg ataccgcaag ataagaaaga aatattgtca tttctttcat
     1801 atactgtcac ttctgcttct ggatgatttt ttaaaatact ttttacagct gcagtacctg
     1861 catgagtaca gccaattact actactttca tttatggtcc ctccgattaa cattttgtcc
     1921 ttcgacaaaa ctactataac agaaataaaa cggatacaac agtttaatgc ttgtgaattc
     1981 acaaaggttt gtttgtagtt tcattccttt tcaagcataa gaacccaata tctacaagat
     2041 tcttattgtg ataatattca ctttgatatc taagattgtg aataaaagac aaaaaaataa
     2101 aaaagctagc gtttttttac acagctagct ttttttaggt ataacactta tagaaactgt
     2161 ttttacattt gcgccttttt tgctgactct ttacaatccg gacagagtcc atacaattcc
     2221 aaccgatgtt cattaatctc aaaatcagtc agtctgctcg cagccatttc tatatcttca
     2281 agccctgggt aatgaaaatc aacaatctta ccgcagtttt cacaaattac atgataatga
     2341 cgctttgaac tgaaatcaaa tcggctagaa gcatcgccgt acttcatttc ttgaacaaat
     2401 ccaatcgaag taaataagcg aaggttattg taaacagtcg caacactcat atttggaaac
     2461 cgatgttcaa gtgctttata aatctcatca gccgttggat gggaatgatg ttcaatcaaa
     2521 tattctaaaa ccgcgtaccg ttgaggtgtg attcgaatgt tctctttttt taacttttcg
     2581 ataacctctt cgactaactt attttccatg taaacacccc acgttgttat tctcgtctta
     2641 tcatactatt tttacatttt aaaatccact taacatgaaa agaaaattca attttattgt
     2701 attttaatga aaaaagacgt aaaacagtcg cgttcactat tttacgtctc tattcaaata
     2761 atttttctca tcataaattt ttgtgtgttc ttttcatctt taaaagtttt ttacataaac
     2821 ctatctggag aaaaaccaag tgccttatat ctcttggtaa agggctgtag ataaataacg
     2881 ttcaccattg tcgggaacca cagctaatac cttcttgcct tttcctaatt cttttgcgac
     2941 ttttagcgcg gcagcaattg cagcgccaga tgagatgcct actaaaatcc cttcttttcg
     3001 cccaacttct ctagctgttt ccatcgcttc gtctccacta atagaaagga ctttatcata
     3061 cacagaagta tccagtgttt taggaacaaa acctgtaccg attccttgga ttttgtgagg
     3121 tcccgcttct ttcccttcta aaatagcaga ttctgctggt tctaccccga ttaattcgat
     3181 tttcggatac acacgtttta attctcggcc agcgccagtg atcgttcctc ctgtaccgat
     3241 acctgcaaca aacgcgtcca agccatttac tccaaatgct tgatgtatct caggaccagt
     3301 cgtcttctca tgcacaagag gatttgcttc attttcaaat tgtaatggta aaaagtagcc
     3361 gttttctttt gccaaacgtt ctgcttctct gattgatcca gaaataccat ccgcaccagg
     3421 agtcaaaatc aactctgcgc cgtacccttt catcaataaa cgtctttcga tactcatcgt
     3481 ctcaggcatc acgatgatta ctttatatcc ttttgcaact cctaccatcg acaatccgat
     3541 tcctgtattt ccagaagtag gttcaatgat agtatcacct ggtttcagta gaccatcgtg
     3601 ttctgctttt tctatcatac ttaaagcaat acgatctttt acacttcctc ctggattaaa
     3661 aaactcaagc tttacaaaaa catctgctga atcttctggt acgattttat ttaatttgac
     3721 gattggcgtt ttgccaatca attcggtgac tgagttatag atagctgtca tcttcaattc
     3781 ctccttgtct ttgctcttct tacttatcat aatacaatct gttcatttat ggcgagtgat
     3841 tcgtctttga ctagcaaaaa aaagacaaag atataccata tatggtagtt tctttgcctt
     3901 tttatctaaa tcaaacgttt agttcttgac caactaataa agaataactg gacatagaca
     3961 tgccattttt cgccattaga tcatcaacag acatgccaaa tttttgtgca atatcccata
     4021 gcgtatcacc tgacacgact gtatatgtct gaccattatt tgtatttaca tctgttgttg
     4081 aagatgatga cgttgaagtc gtcgtagtag tcgttgttgt tgtacttgtg gtagccgaac
     4141 ctgttgatgg cgtatcaaaa cgggttaaat tataagtatt aatcaatgag ttcaatttgg
     4201 ccccataatt tggatcggta gcataacgac ctgtcaagta cgctgttgca tcataaaaac
     4261 ttgttgtatt gcttttccac acgccagcat aatgataatc gcccgtggaa aaactggttg
     4321 aacgaagaac attcgcatgg tcttggaagg attcccaata agaagaatat tttcggaatg
     4381 gttcattcat cgttacccat tgtccgttac atattctttg gttgccatat aaacgacctg
     4441 tccgttatag cttcctttca caccgaatag attataattt ggcgcactag ctaatccgga
     4501 agtgccatat gcactttcca acaacgcttg agcgatcatg actgaagcat acaagtcatt
     4561 acttgcagca acttctgacg cagaccagcc gatttgatcg ataaacgctt gaggatcagc
     4621 aactgtttga gatccttgtt catctgcatg aacatgttgc ggtaccgctg taaccatcat
     4681 cggcccaact aaaagactcg tt
//
LOCUS       DAZKLD010000171         4662 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.174, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000171 DAZKLD010000000
VERSION     DAZKLD010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4662)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4662)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4662
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.174"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            162..392
                     /locus_tag="ACG8KR_002664"
     CDS             162..392
                     /locus_tag="ACG8KR_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 family protein"
                     /protein_id="HFQ0201975.1"
                     /translation="MQVYGIDAVLRIVSHLAFIYLAFWSLRSLRIETFFRPMHNTQIR
                     MAIVLFSIFLGYTASNFFLEVIALCRNLFVSL"
     gene            489..1790
                     /gene="murA"
                     /locus_tag="ACG8KR_002665"
     CDS             489..1790
                     /gene="murA"
                     /locus_tag="ACG8KR_002665"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFQ0201976.1"
                     /translation="MEEIIVRGGNQLNGTVRIEGAKNAVLPILAASLLAEEGITTLDN
                     VPILSDVFTMNQVIRHLNVDVDFDEQKNQVTIDASRQLEIEAPYEYVSQMRASIVVMG
                     PLLARNGHAKVAMPGGCAIGKRPIDLHLKGFQALGAKIIQKNGYIEAIADELIGNTIY
                     LDFPSVGATQNIMMAAVKAKGTTIIENVAREPEIVDLANILNKMGAQVYGAGTETMRI
                     EGVDHLHAVNHSIVQDRIEAGTFMVAAAMTQGNVLIADAISEHNRPLISKLIEMGAEI
                     IEEEGGVRVIGPKHILPTDVKTMPHPGFPTDMQAQMTAIQLVAEGTSVVTETVFENRF
                     QHLEEMRRMNAHVKIDGNVAIMDGNHELQGAEVYATDLRAAAALVLAGLKANGITRVR
                     NLNYLDRGYYNFHIKLQQLGADVERVDMDQTSAEKTAQTIA"
     gene            1803..1979
                     /locus_tag="ACG8KR_002666"
     CDS             1803..1979
                     /locus_tag="ACG8KR_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFQ0201977.1"
                     /translation="MSNQRYIILTLIKILVVILLLILLFVAGTMIGYGVIGGGNPFKV
                     FQPSLWIHIRDFFH"
     gene            2114..2410
                     /gene="yidD"
                     /locus_tag="ACG8KR_002667"
     CDS             2114..2410
                     /gene="yidD"
                     /locus_tag="ACG8KR_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HFQ0201978.1"
                     /translation="MKKILIYLVRGYQRFISPAFPPSCRYYPTCSNYMIKAIQTHGAL
                     KGTVMGTARILRCHPFVKGGIDYVPLTFQLTRNKKDKKEPDYKKVVKNTSSTER"
     gene            2594..3061
                     /locus_tag="ACG8KR_002668"
     CDS             2594..3061
                     /locus_tag="ACG8KR_002668"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0201979.1"
                     /translation="MIEELNKKDLPWSLLLDADPDKEKVQVYVSRGSGWIWKEKEKTI
                     GVLIYVAREKEYEIVNVAVAPTYQGKGIGGKLLETAFQKLSQLASSQTQIIIRTGSTS
                     SAALHLYQKMGYVEIGRVKDYFIHHYSEPIFENGDQLRDQVILAIHLPKNSFN"
     gene            3146..4030
                     /locus_tag="ACG8KR_002669"
     CDS             3146..4030
                     /locus_tag="ACG8KR_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFQ0201980.1"
                     /translation="MGILVFDFGGSAVKYGCWDGKEIKGKGQFATPESWEEMKAQLFQ
                     VYKKILVSFEVEGIGISSPGVVNNEKQVIEGISAIPYIHGFNIFRDLETLFQLPVTIE
                     NDANCAGMAEFYEGAARDYQDVAFVVAGTGIGGALFTKGQINKGAHLYGGEFGLMFLN
                     GDKTFSKLGTAVQMAWRYCERKGLDKNSYTGKDVFELAETGDAIAKEEVESFYTYLTK
                     GLFSIQFSFDPEVIVLGGGVSAKEGLIDEIKSRMKKLTEQFDLHDFEPQILLCEYRND
                     ANLVGAAANYLAVRQGEN"
     gene            4144..4380
                     /locus_tag="ACG8KR_002670"
     CDS             4144..4380
                     /locus_tag="ACG8KR_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HFQ0201981.1"
                     /translation="MMSKKNKDVEVRIEEVKKDIKGKNYEVTQLFIGKKMIGEILAYG
                     PKEYEIFFGEEDFGKEKSLENAIETVIRHWNLHE"
     gene            4468..4548
                     /locus_tag="ACG8KR_002671"
     tRNA            4468..4548
                     /locus_tag="ACG8KR_002671"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4502..4504,aa:Tyr,seq:gta)
     gene            4555..4626
                     /locus_tag="ACG8KR_002672"
     tRNA            4555..4626
                     /locus_tag="ACG8KR_002672"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4587..4589,aa:Gln,seq:ttg)
BASE COUNT         1571 a          728 c         1021 g         1342 t
ORIGIN      
        1 ttttgtctca gtcccttttt ttgcatattt ttagcattta tttcctaaaa aatgtaaggg
       61 gattcctata tttttaagta tacttgtttt tccttttata cattttatgg tattatcatt
      121 ttattagtta ttataaaaca atcgttagaa aggacgtcat tatgcaagta tacggaattg
      181 atgcggtgct tcgaatcgtg agtcatttgg cgtttatcta cttagccttc tggtctttac
      241 gttcgttacg cattgaaact tttttcagac caatgcataa tacgcaaatt agaatggcaa
      301 tcgtactttt ttcaattttt ttagggtaca ctgcaagtaa ttttttctta gaagttatcg
      361 ctttatgccg aaatcttttc gttagcctgt aaattttaaa aaaaggtact attttaaaag
      421 caaggatgtt ataatagaaa agattttgag taagtgtatg ctcattggaa ttttttggag
      481 ggaacaacat ggaagagatc atcgtaagag gtggcaatca attaaatgga acagtaagaa
      541 ttgaaggagc taaaaatgct gttttgccta ttttagctgc tagtttgtta gcagaagaag
      601 gtattacgac attagacaac gttccgattc tttctgatgt cttcactatg aatcaagtga
      661 ttcgtcactt aaacgtagat gtagattttg atgaacaaaa gaatcaagta acaatcgatg
      721 cttcaagaca attagaaatc gaagcgcctt atgagtatgt tagtcaaatg cgtgcttcaa
      781 tcgttgtgat gggtccgcta cttgccagaa atggacatgc aaaagtagct atgcctggtg
      841 gatgtgcgat tggtaaacga ccaatcgatt tgcatttaaa aggatttcaa gctttaggag
      901 caaaaattat tcaaaaaaac ggctatatcg aagcaattgc ggatgaattg atcggtaaca
      961 cgatttattt agatttccct agtgtaggtg ctactcaaaa tatcatgatg gctgccgtaa
     1021 aagcaaaagg aacaaccatc attgaaaacg tagctcgcga accagaaatc gttgatttag
     1081 caaacatttt gaataaaatg ggcgctcaag tctatggagc tggtactgaa acaatgcgta
     1141 ttgaaggcgt ggatcattta catgcagtca accactcaat cgtacaagat cgtatcgaag
     1201 caggtacctt tatggttgct gctgcaatga cacaaggcaa tgtgctgatt gcagatgcta
     1261 tctctgaaca taaccgccca ttgatttcta aattgatcga aatgggtgca gaaatcattg
     1321 aagaagaagg cggagtccgt gtcatcggac caaaacatat cttacctaca gatgtaaaaa
     1381 cgatgcctca tcctggtttc cctacagata tgcaagctca aatgacagct atccaattag
     1441 ttgcagaagg tacaagtgta gtgactgaaa cagtgtttga aaatcgtttc caacatttag
     1501 aagagatgcg ccgtatgaac gctcatgtca aaatcgacgg aaatgtagca atcatggatg
     1561 gaaaccatga attgcaaggt gcagaagtat atgcaacaga cttgcgggca gcagcagcat
     1621 tagttttagc tggcttgaaa gcaaatggaa tcacacgtgt gcgtaacttg aattatctag
     1681 accgtggata ttataatttc catatcaaac tacagcaatt aggtgcagac gttgaacgtg
     1741 tagacatgga tcaaacttct gctgaaaaaa cagcacaaac gattgcttaa ggaagtggct
     1801 gaatgagtaa ccaacgatat atcatattaa ctttgataaa gattttagtg gtgatcttat
     1861 tgcttatcct tctttttgtg gccggaacga tgatcggata tggtgtcatc ggtggcggca
     1921 atccatttaa agtattccag ccaagtttgt ggattcatat tcgagacttt tttcattgat
     1981 aaataagaat cacagtggtt ctagtcgata tgaagtgagc catgaagcag aacatgacaa
     2041 aagaaaacag aagggaacgt tgacgttagt cgcgatccct ttttttagta aaagaaaaga
     2101 aggaatctaa ttgatgaaga agatattgat ttatttagtt agaggatatc agcgctttat
     2161 ttcgcctgca tttccaccta gctgccgtta ttacccaacc tgttccaatt atatgataaa
     2221 agcaattcaa acccatggtg cactaaaagg gacggtaatg gggactgcac ggatcttacg
     2281 ctgtcatcct tttgtcaaag gcggtatcga ttatgtacca ttgacattcc aattaacgcg
     2341 aaataagaaa gataaaaaag aaccagatta taaaaaggtt gtgaaaaata cgagtagcac
     2401 tgagcgataa agaaacaatt tggaaaatag cttcttatat tttgcaaaat tgtgagttat
     2461 tgccgaagtg ttggattttg aacaccgtca aaaaaaggtt gtgaaaaata cgggtagcac
     2521 tgagcgataa agaaaaagag gaaaattatt gtcaaagtaa caactccgca actacttata
     2581 tgggggacaa aaaatgatcg aagaactaaa caagaaagat ttaccatggt ccttgttatt
     2641 agatgcagat cctgataaag aaaaagttca agtatatgtt tccagaggga gtggctggat
     2701 atggaaagaa aaagaaaaaa caataggtgt actgatctat gtggcaaggg aaaaagaata
     2761 cgaaatcgtc aatgtagcag tagccccaac gtatcaagga aaggggattg gcggaaaatt
     2821 acttgagaca gcttttcaga agctttcaca actcgcttcc agtcaaactc agatcatcat
     2881 acgaaccggc agtactagca gtgcggcgtt acatctctat caaaaaatgg gctatgtgga
     2941 gattggtcga gtcaaagact attttatcca tcattactct gagcccattt ttgaaaatgg
     3001 agatcaatta agggatcaag taattttagc aattcactta cctaaaaata gctttaacta
     3061 ataaactttc aattcgagaa aatgctttca aatgctaaat agatacatta caatagcttt
     3121 agcaaacaaa aaggagaata tttttatggg aatattagtt tttgattttg gtggatcggc
     3181 tgtcaaatat ggttgttggg atggcaaaga gattaaaggt aagggacaat ttgcaacgcc
     3241 tgaatcttgg gaagaaatga aagcgcaatt atttcaagtt tataaaaaaa tactcgtttc
     3301 ttttgaagtc gaaggaattg gaatcagctc tcctggagta gtgaataatg agaaacaggt
     3361 gattgaggga atcagtgcga ttccttatat tcatggattt aatattttcc gagatttgga
     3421 aactctgttt caactcccag taacgattga aaatgacgcc aactgtgccg gaatggctga
     3481 attttatgaa ggtgctgccc gtgattatca agatgttgct tttgttgtgg caggaactgg
     3541 gattggcggg gcgttgttca cgaaaggaca aatcaataaa ggggcacatc tttatggtgg
     3601 agaatttggg ttgatgttct taaatggcga taagacattc agtaaacttg gtacagcggt
     3661 acagatggct tggcgttatt gtgaacgcaa aggtctcgat aaaaattctt ataccggaaa
     3721 agatgtcttt gaattagcag aaactggaga tgcaatagca aaagaagaag ttgagagttt
     3781 ttatacgtat cttacaaagg gattattcag tattcaattt tcgtttgatc cagaagtgat
     3841 cgtattaggc ggcggtgttt ctgcaaaaga agggctcatt gatgagatca aaagccgaat
     3901 gaagaagctg acagaacaat tcgatctgca tgatttcgaa ccgcagattt tattatgtga
     3961 atacagaaac gatgctaatc ttgtaggtgc agcagcaaac tatctcgctg tacgtcaagg
     4021 ggagaattaa gagctttaga gaaggtgcag cttgcataaa agaagtgaat gaaatgaata
     4081 ggagattttt acctaaggaa tgttttattt gctgcagtat gataaaatat ctgagaggtg
     4141 aaaatgatgt caaaaaaaaa taaagatgtt gaagtgcgta tagaagaagt gaaaaaagat
     4201 atcaaaggta agaattatga agtcactcag ttatttatag ggaaaaaaat gattggtgag
     4261 atcttagcct atgggccaaa agaatatgaa attttctttg gtgaagaaga ttttggaaaa
     4321 gaaaaaagtc ttgaaaatgc gatagaaaca gtgattcgac actggaatct gcacgaataa
     4381 aaaaatatca aaaaaacttt caataaaggg ttgcaaaaat ctggaaaatt aggtattata
     4441 actaagtcag gttgatacaa acctttcgga ggagtagcga agtggctaaa cgcgacggac
     4501 tgtaaatccg ttccttaggg ttcagtggtt cgaatccact ctcctccatt tcattggggt
     4561 atagccaagc ggtaaggcaa gggactttga ctccctcatg cgttggttcg aatccagcta
     4621 ccccagtata ccacaatcgt aagctctcgt tagttcgaga gc
//
LOCUS       DAZKLD010000172         4589 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.175, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000172 DAZKLD010000000
VERSION     DAZKLD010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4589)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4589)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4589
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.175"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            189..2858
                     /locus_tag="ACG8KR_002673"
     CDS             189..2858
                     /locus_tag="ACG8KR_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HFQ0201982.1"
                     /translation="MKKNLKSFFRENGRYAAASFLIPFLILALVYLSIGIYPGSSRSI
                     LASDAFSQFSNFHASFRNVLLGKQSIFYTWNASLGLNYLSLISYYLGGIFTPLVFFFP
                     NQMMPDALYVLTLLKVGCAGLAFWFLARTFKIPRWGHVALSVSYALISFITAHSEIIM
                     WLDAFVYLPLVILGIHRVMDLKRPKLLFISYLLLFLSSFYMGFMIGVFSFLYFIARLL
                     TNWSEYKKTIVPYGITSLLAGGASMIIVLPAVLDLRSNGEALSQITTLKTEATSYLDL
                     IMKNMIGVYDTTKYGSIPFVYAGLLPLVLCLFYFASRKVPLKNKFLYGSLFAILIASF
                     YLVPLNLFWHGMHAPNMFLFRYAYLFSFLVLLLAGKGWEKIEQDDRGLLLGIILVVAA
                     LFAVAWGIKPEGSYTYVTMTSFVLTVVFLGIYAFAVGFYQHKKLSFNHLSILLLLIMS
                     AEALVNTNSMIHGILDDWNYASRSLYTDPYPDLKALVNETKKDSDSFYRLENLDPVSA
                     NDSINYGYSGISLFSSIRNRNSSSYLDKLGFRSRGTNLNIRYNNNTLLMDGFTGIKYN
                     ISKNESTFSKYGFRQAGQSGDYTLYQNVNALPLAFTAPLSVNDVEQPVTDNLTSQTNL
                     INTLSGLNEQYFTFYTPTLKNQLNTTITKTTTGVTYSETADNQPKELTWEVHVPANTQ
                     AYLSLFPTNFAQLESSSATITVNGTSRKTQINISGQYYDLGYYPQETTVTFTASFYGT
                     KEVSFMEPKVVGLDVVAYQAAMNQIKEKGVEMEATGRKASGTVNTAEDSMLVTTIPYD
                     KGWKAKIDGKPVKVSNFKDAFLMIKVPAGKHTVTFSYLPEGFTIGAILFVLCIALFIL
                     YVWYLDRNRPFYGTIDPAIEEKPRNTHRRRRRK"
     gene            complement(2949..3563)
                     /locus_tag="ACG8KR_002674"
     CDS             complement(2949..3563)
                     /locus_tag="ACG8KR_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFQ0201983.1"
                     /translation="MINATDEILSVNKVFYTVPAEKMSRVSSDINTITSRIGLTKAMH
                     MVLKYNQEDPPVPKTYEAIDLIIVDEIDRLKLQHLEQLRDIYDRNDLAMVLIGMPGIE
                     KRLSRYPQLYSRIGFAHEFDNLSKDETHHILEYKWADLGIDIKLEDFSDYEAITTIIK
                     ITKGNFRLIHRLFAQIDRILRINQMDKITVEVVEAARDSLVIGI"
     gene            3683..4222
                     /locus_tag="ACG8KR_002675"
     CDS             3683..4222
                     /locus_tag="ACG8KR_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0201984.1"
                     /translation="MKKLFSALFILLFGFIGIGTSGNIVKASTMVEQTSEMAKTFSQL
                     TPEEQSYFKSKGFSDKDTFYTQKIEPQDPKTRFSANVLRLTASTKKITNTQGRTEYII
                     TSPNSAMYRISTRLSLGSAKSYTQTVNLANSYGYGGGMYFTFTGKRQYYSVKLAMQVY
                     NTFGVGSVSCTAGGLTIGK"
     gene            4219..4557
                     /locus_tag="ACG8KR_002676"
     CDS             4219..4557
                     /locus_tag="ACG8KR_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0046685 - response to arsenic-containing
                     substance [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenic metallochaperone ArsD family protein"
                     /protein_id="HFQ0201985.1"
                     /translation="MIISYYVLNRSDDEGLFVFQSLHEDTMLKEFYNRIKNKQIETNL
                     YFLSDSILPFISNPKILNLLQTKDLKSLPIIVIDEEIVKYGAYLSVEELEKLTNIGMS
                     IQEDNGVHGT"
BASE COUNT         1422 a          789 c          860 g         1518 t
ORIGIN      
        1 aaaattttta agggtgggac ttttgattcc tacccttttt ttgcgtttat ttttgttata
       61 cgaacaaatt atgaagttat ttattctaaa ttttattttt ttgtgaaaat tagactcttt
      121 ttaagcagag aaaaacaagg atatgataaa ctgaatacaa cttcatagaa atttggtgga
      181 aaataaatat gaaaaaaaat ttaaagtcat tctttagaga aaacggacgc tacgcagcag
      241 ctagtttcct gattccgttt ttgattttag cacttgttta tctaagtatc gggatctatc
      301 ctggaagcag cagaagtatt ttggcaagtg atgccttttc acagttttct aattttcatg
      361 ccagcttccg aaatgttctt cttggaaagc aaagtatttt ttatacatgg aatgcttctc
      421 taggattgaa ttatttatct ttaatttctt actatctagg cgggatcttc acgccactgg
      481 tgttcttttt tccaaaccag atgatgccag atgcactgta tgttctgact ttgcttaagg
      541 tcggctgtgc cggattggct ttttggtttc ttgcacgtac gttcaagatt cctcgctggg
      601 ggcatgttgc acttagcgtc agttacgcat tgatctcatt tatcactgca cattcggaga
      661 tcatcatgtg gctggatgca tttgtttatc ttccattagt gattttgggg attcatcgtg
      721 ttatggatct taaacggccg aaacttttat ttatcagtta tttactcttg tttttatcca
      781 gtttttatat gggttttatg attggcgtat tttctttctt atattttatc gcccgtctat
      841 tgacaaactg gtcagaatat aaaaaaacga ttgttcctta tggtatcact tctttactgg
      901 caggcggcgc ttcaatgatc attgtattgc cagcagtgct ggatcttcgg tcaaatggcg
      961 aggcactgag tcagatcact acgttgaaaa cagaagcgac ttcttatctg gatctaatca
     1021 tgaaaaatat gattggtgtt tatgacacga caaaatacgg atcgattccg ttcgtttacg
     1081 caggtctgct accattagtc ctctgtctgt tttattttgc cagtcggaaa gtaccgttga
     1141 aaaataaatt tttgtacggc agtcttttcg ccatacttat cgcaagtttc tatcttgttc
     1201 cattgaattt gttctggcat ggtatgcatg caccgaatat gtttttattc cgttatgcct
     1261 atctgttctc tttcttagtg ctcctgcttg caggaaaagg atgggaaaag atagaacaag
     1321 acgatcgtgg gctgttgtta ggaatcattt tggtcgtagc tgctctcttt gcggtggcat
     1381 ggggcatcaa acctgaagga agctacacct atgtgacgat gacttccttt gtattgacag
     1441 tggttttctt agggatttat gcttttgctg tgggatttta tcagcataaa aaactttcat
     1501 tcaatcatct gagtatattg ttattattga tcatgtctgc tgaagcactg gtcaatacga
     1561 acagtatgat ccatggcatt cttgatgatt ggaattatgc ttcccgcagt ttatatactg
     1621 atccttatcc tgacttgaaa gctcttgtaa atgaaacaaa aaaagattcc gactcgtttt
     1681 accgcttgga aaatctggat ccagtatctg caaacgattc gatcaactat ggttacagtg
     1741 gtatcagttt gttttcttct ataagaaatc gtaattcttc ctcttacctg gataaattag
     1801 gtttccgttc aagagggaca aatttgaata tccgttataa caataatact ttgctaatgg
     1861 atgggtttac aggaatcaaa tataacattt ctaaaaacga aagcacgttc agtaaatatg
     1921 gcttccgcca agctggtcaa agtggcgact atacactgta tcagaatgtc aatgcattgc
     1981 cactagcatt tactgcaccg ttgtccgtta atgatgttga gcagccagtg acagataatt
     2041 tgaccagtca gacaaacttg atcaatacat tgtctggttt aaatgaacag tattttacat
     2101 tctatacacc tactttgaag aatcagttga atacgacgat cacaaagaca acgactggcg
     2161 tcacttactc agaaacagca gataaccagc caaaagagct cacttgggaa gtccatgtgc
     2221 ctgcaaatac acaagcttac ttaagtttgt tcccaaccaa ttttgcacaa ttagaaagtt
     2281 ctagtgcgac gatcactgtc aatggtacat cgcgaaaaac gcaaatcaac atttctggtc
     2341 aatactatga tcttggctat tatcctcaag agacaacagt gacatttact gcaagttttt
     2401 atggaacgaa agaagtaagt ttcatggagc ctaaagtcgt tggtctagat gttgtagcct
     2461 atcaagccgc aatgaatcag atcaaagaaa aaggtgtcga aatggaagcc actggcagaa
     2521 aagcttctgg gactgtaaat accgctgaag attccatgtt ggtgacaact attccttatg
     2581 acaaaggatg gaaagctaaa attgatggga aaccagtcaa ggtcagcaat ttcaaagacg
     2641 cattcttgat gataaaagtt ccagcaggta aacatactgt cactttcagt tacttgcctg
     2701 aaggatttac gataggtgca atcctttttg tcctatgtat tgcattgttt attctttatg
     2761 tctggtacct tgaccgcaat cgcccctttt atggcacgat tgatccagca atagaagaaa
     2821 aaccgcgaaa tacacacaga agaagacgaa gaaaataaag aaacgatgga atctgtgtca
     2881 ctcttctatt taaatggcaa aaatttcaat ctttaaatga caaaaaccac ctgatttttg
     2941 gtggtttttt atatacctat gactaaacta tctcttgctg cttcgacaac ttctacagtg
     3001 atcttatcca tttgattgat tcttaaaatc cgatcaatct gcgcaaataa acgatgaata
     3061 agcctaaaat ttccttttgt gatcttaatg atagttgtga tagcctcata atcagaaaaa
     3121 tcttctagct tgatatcaat tcctaaatct gcccatttgt attccaaaat atgatgtgtt
     3181 tcgtccttac taaggttatc aaattcatgt gcaaatccta tgcgagagta aagctgcgga
     3241 taacgagaca atcttttttc aataccaggc ataccgataa gaaccattgc cagatcattt
     3301 ctatcgtaaa tatctcttag ctgttctaaa tgctgaagct ttaaacgatc aatttcatcg
     3361 acaataatta agtcaattgc ttcataggtt ttaggcacag gaggatcttc ttgattgtat
     3421 tttagtacca tatgcattgc tttagtcaga cctattctac tagttattgt gttaatatcc
     3481 gaactgacac gagacatctt ttcagctggt acagtataaa aaactttatt tacacttaat
     3541 atttcatctg ttgcatttat caagaacaag aaaaagtctg aatattcaaa aaaaataacg
     3601 gtgtacattt ttaaaaggag atgtataatc gagatttaga aagcgttttc ctcaaaattt
     3661 aatgaattag gaagtgatta ctatgaaaaa attatttagc gcacttttta ttctgttatt
     3721 tggctttatt ggtattggga cttctggaaa tatagttaaa gctagcacga tggttgaaca
     3781 aacaagtgaa atggcaaaaa cttttagtca attaacacca gaagaacaaa gttacttcaa
     3841 atccaaagga tttagtgata aagatacttt ttacactcaa aagatagagc ctcaagatcc
     3901 gaaaacacgt ttttctgcaa atgtattgcg gttaaccgca tctactaaaa aaattactaa
     3961 tacgcagggg cggacagaat atattataac aagtcccaat tcggcgatgt atcgtatttc
     4021 tacacgcttg agtttaggta gtgcaaaatc atatactcaa acagtaaatc tagctaattc
     4081 ttatggttat ggtggaggaa tgtattttac atttactggg aaaagacaat attacagtgt
     4141 aaaattagct atgcaagttt ataatacatt tggtgtaggg tcagtctctt gcactgcggg
     4201 aggattgaca ataggaaaat gataatttct tactatgtct taaatagaag tgatgatgaa
     4261 ggactcttcg tttttcaatc attacatgag gatacgatgc taaaagagtt ttataatcgg
     4321 attaagaata aacagataga aacaaattta tattttttaa gtgactctat cttgcctttt
     4381 attagcaacc ctaaaattct taacttgttg cagacaaaag atttgaaaag tttaccgatt
     4441 attgtcattg atgaggaaat tgtaaaatat ggcgcttatc taagcgtaga agaattagaa
     4501 aaattaacta acattggaat gagtattcag gaggataatg gggtacatgg aacatagaat
     4561 gaatatgcac agttgagaac ggaatttga
//
LOCUS       DAZKLD010000173         4513 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.176, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000173 DAZKLD010000000
VERSION     DAZKLD010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4513
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.176"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(70..411)
                     /locus_tag="ACG8KR_002677"
     CDS             complement(70..411)
                     /locus_tag="ACG8KR_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFQ0201986.1"
                     /translation="MKPNEIVTVYVAFTDKNGGKRRPILVVSDKEDRVEFFGITSQYE
                     NKSDQIKRVYFPIAEWEKVGLKKQSWIDVGSLKAIPKSRENISFKKVGMLSAIDTKKL
                     NRFIERLQRKV"
     gene            complement(401..682)
                     /locus_tag="ACG8KR_002678"
     CDS             complement(401..682)
                     /locus_tag="ACG8KR_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HFQ0201987.1"
                     /translation="MKVKNPKEKSRIQVGIDKDLKENAEMILEELGLNPTTAITILYK
                     QVVARGEFPVEIKLSEEEKQGIRLQQLTMDMPVDVLDTDEKLEEWFNEA"
     gene            complement(789..1373)
                     /locus_tag="ACG8KR_002679"
     CDS             complement(789..1373)
                     /locus_tag="ACG8KR_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0201988.1"
                     /translation="MNENKMVLSEYARLRTINWLNGMQSSGAFDGFDEENMDFESLSN
                     EDIRYYFIEWYFTEPDEEVEDEPELILERQRILFSEQDSKRLYGLYDLQLKGLKAQYV
                     ADSYDDMVEQGADRVVTYSEDDEEEWENYSDDEICFNDGYMPIEITEEEYDKVSDSDE
                     IGMCTVVSGLDIDVQRVEVAYQKMKERIKSKVES"
     gene            complement(1390..1629)
                     /locus_tag="ACG8KR_002680"
     CDS             complement(1390..1629)
                     /locus_tag="ACG8KR_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0201989.1"
                     /translation="MTKFKSKGMQWCSYYYVGNDEELLRKAKQKGYPDKMLKQIGYYM
                     PEERIEKESCKVLFDSEQLLVLLTQNKLICQFENN"
     gene            complement(1648..1896)
                     /locus_tag="ACG8KR_002681"
     CDS             complement(1648..1896)
                     /locus_tag="ACG8KR_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0201990.1"
                     /translation="MEGMDFIDHEDLIDFGYTWKGMVGISRSLANAFYERNYAVYVLY
                     DDNTESLVDEEYKLDLENVLYGIEKEDLAKYIFSWLGQ"
     gene            complement(1911..2477)
                     /locus_tag="ACG8KR_002682"
     CDS             complement(1911..2477)
                     /locus_tag="ACG8KR_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0201991.1"
                     /translation="MFEKRKDQFPNLEAKSQKVIDGEMIELFQKSYGTIETTASCKYY
                     FQGKRNTFKGFADSDEELAKKIIDWLVSSEHVAREDTVRTTIEGEVLTGTRCVCCNHE
                     FVEDEGCYRINEYGDFICQSCHEIEFPTERSWFEYMLQDYEMETENGEVVFCTKEELS
                     KRSDDEIEDWCVWLVGTDDCVAYYTTAE"
     gene            complement(2478..3023)
                     /locus_tag="ACG8KR_002683"
     CDS             complement(2478..3023)
                     /locus_tag="ACG8KR_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0201992.1"
                     /translation="MGERTQALITVKYGNSKTKVSVSIHYQWGYGRFMLMDVLNQGIY
                     LINKAYDLRDESREELAKGIFDRSTGVINFVTYESGVSAYDETGFGGRYEPYQETIED
                     VMKFSDNNDGYAHLEMVFDENGFSLDDKTRLRLFDFEGQQIGLNKFCESGKEFATKDF
                     IKGYRLLLENYGINLEEESVK"
     gene            complement(3049..3816)
                     /locus_tag="ACG8KR_002684"
     CDS             complement(3049..3816)
                     /locus_tag="ACG8KR_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
                     /protein_id="HFQ0201993.1"
                     /translation="MRDRILEVNQEMVNKLTNISGNYEKYDGTLLDNYIFHDTQNIRI
                     GRSKPRAFILIKEKYLNEWNSTYEMVMTDNFEKVLDFRNALLGSDMGNSSLESIEVEN
                     KTENEGEGLIKNVLKIEQKYISEDRSFYRVTSSVGLANFATNLIGNNASESLLVMCLN
                     TKNEVIAYSEVFKGGINQSVAIPREIFQLALLNNSAKIAVSHNHPSGYEFPSENDKSF
                     TTNLKNAGKTIGIELLDHIIVTSKNTSYYSFQENGSL"
BASE COUNT         1277 a          979 c          568 g         1689 t
ORIGIN      
        1 aatatacata aatacgttta tataaacgta tatcttaata tacatataaa caaaaaaaga
       61 aaagccttgt tagacttttc tttgtaatct ctcgataaac ctatttagct ttttcgtatc
      121 aatagctgat agcataccaa cctttttaaa tgatatgttc tctcggcttt tgggaatagc
      181 ctttaaagac cctacgtcaa tccatgattg cttctttaga cctacttttt cccattctgc
      241 aataggaaaa tacactctct ttatttggtc tgatttattc tcatattgac ttgtgattcc
      301 aaaaaattct actctatctt ctttatcact aaccaccaaa ataggtcttc ttttaccacc
      361 atttttatct gtaaatgcaa catagaccgt tactatctca ttaggcttca ttgaaccact
      421 cttctaactt ttcatctgta tctagaacat caactggcat atccatagtt agctgttgca
      481 atctgattcc ttgcttttcc tcttcgctta gctttatctc tactggaaac tcacctctag
      541 cgaccacttg cttataaagg attgtgattg ctgttgttgg atttaaccct agctcttcca
      601 atatcatttc tgcgttttct tttaaatcct tgtctattcc gacttgaatt ctgctttttt
      661 cttttggatt ttttaccttc atttcgttca cctcttttga gtataatata ctcattatta
      721 tatccattaa ctacatttat gattatatta tactcttaat tagctgcttt gcaaacaatc
      781 caatcaaatc aagattctac tttacttttt atacgttctt tcatcttctg gtatgcaacc
      841 tctactcttt ggacatctat atccaatcca cttacaaccg tacacatacc aatctcatcg
      901 ctatccgaca ctttatcata ttcttcttcg gtgatttcaa taggcatata cccatcatta
      961 aagcatatct catcatctga atagttttcc cattcctctt catcatcttc tgaataagtg
     1021 acaaccctat ctgccccctg ttctaccata tcatcgtaac tatcagcaac atactgcgct
     1081 tttaatcctt tcagttgtaa gtcatataaa ccatacaacc tcttactatc ttgttcactg
     1141 aataaaattc tttgacgttc taaaattaat tctggttcat cttccacctc ttcatctggt
     1201 tctgtaaaat accattcgat aaaataatat ctaatatcct cattcgataa actttcaaag
     1261 tccatattct cctcatcaaa tccatcaaaa gcgccactac tttgcatacc attcaaccaa
     1321 ttaatggttc tcaatcttgc gtattcactt aataccatct tattctcatt catggttctt
     1381 caccccatat taattatttt caaattgaca aatcaattta ttttgagtta acaaaactaa
     1441 tagctgttca ctatcaaaaa gaaccttaca agattctttt tctattctct cttctggcat
     1501 gtaatagcca atctgtttta acatcttatc tggataacct ttttgtttgg cttttctcaa
     1561 aagctcttca tcattcccaa cataataata agaacaccat tgcatacctt tacttttgaa
     1621 ttttgtcatg ttgctaccct ctttcgttta ttgacccaac catgaaaaaa tatattttgc
     1681 taaatcttct ttttctatcc catataaaac attctctaaa tccaatttat actcttcatc
     1741 aacaaggctt tctgtattgt catcataaag gacatagact gcataatttc tttcgtaaaa
     1801 ggcattagca agacttctgc taatacctac cattcctttc catgtataac caaaatcaat
     1861 caaatcttca tgatctataa aatccattcc ttccatgctg attcacactc ctattctgct
     1921 gttgtgtaat atgcaacgca atcatctgtc cctactaacc acacacacca atcttcaatt
     1981 tcatcatcgc ttctcttact caattcctct ttggtacaaa aaactacttc accattttct
     2041 gtttccatct cataatcttg tagcatgtat tcaaaccaac ttctttcggt tggaaattct
     2101 atctcgtggc aactctggca aataaaatca ccatattcat tgattcggta gcaaccttca
     2161 tcttctacaa attcatgatt acaacaaaca catctagtac ctgttaggac ttcaccctca
     2221 atcgttgttc taacggtatc ttctcttgct acgtgttcac tgctgacaag ccaatcaatg
     2281 attttctttg ccaattcttc atcagaatcg gcaaagcctt taaacgtgtt tctctttcct
     2341 tgaaagtagt atttgcaact tgcggtagtt tctatggttc cataactttt ttggaataat
     2401 tcaatcattt caccatcaat cactttttga cttttcgctt ctaaattagg gaactggtct
     2461 tttctttttt caaacactta tttcacgctc tcttcttcta ggtttattcc ataattttct
     2521 agcaataatc tgtatccctt aataaaatct ttcgtagcaa actcttttcc gctttcacaa
     2581 aacttgttta gtcctatctg ttgaccctca aaatcaaaca atcgcaatct ggttttatca
     2641 tctagactaa atccattttc atcgaacacc atctctaaat gagcatagcc atcattgtta
     2701 tctgaaaact tcatcacatc ttcaatcgtt tcttgataag gttcatagcg cccaccaaac
     2761 cctgtttcat cataagcact cactccagat tcataggtga caaaattgat aactcctgta
     2821 cttctatcaa agattccttt tgctaattct tctcttgatt catctcgtaa gtcatatgct
     2881 ttattgatta aatatattcc ttgattcaaa acgtccatca acatgaatcg accatatccc
     2941 cactgataat gaattgacac gcttactttc gttttgctgt taccatattt gactgtaatt
     3001 aacgcttgtg ttctttcgcc catcttgttt tcctcttttc tctaattttt ataaagaacc
     3061 attttcttga aatgagtaat agcttgtgtt tttacttgtt acgatgatat ggtctaacaa
     3121 ttcaatacca attgtttttc ctgcgttttt caaattcgta gtaaaacttt tgtcattttc
     3181 acttggaaat tcatatccgc tcggatgatt atgtgatact gcgatttttg ctgaattgtt
     3241 tagcaaagct agttgaaaaa tctctcttgg tatcgctact gattgattaa ttccaccttt
     3301 gaaaacttcg ctataagcaa tgacctcatt ctttgtattc agacacatca ctagcaaaga
     3361 ttcactagca ttgtttccaa ttaagttagt agcaaagttt gctagaccaa ccgagcttgt
     3421 gactctataa aaacttctat cttcactgat atatttttgt tcgattttca ggacattctt
     3481 aatcaatcct tcaccttcat tctcggtctt gttttcaact tcaatagatt caagactgct
     3541 atttcccata tcagaaccca acaaagcatt tctgaaatcc aatacctttt caaagttatc
     3601 agtcataacc atttcatagg tgctattcca ttcattcaaa tatttctctt tgatgagaat
     3661 gaacgctctc ggtttacttc tgccaattct gatattttgt gtatcgtgaa aaatataatt
     3721 atcaagtaaa gtaccatcgt acttttcata gttcccactg atatttgtta acttgtttac
     3781 catttcttga ttaacttcta aaattctatc tctcatgttg aacgcttctt tctgtttttg
     3841 atttttggtt tttcaaaatt cttttttgtt tggattgttt tagagggagg tggttttttc
     3901 ttcatcgcta tttggttttg cattttatgt ttcttccttt cgttcattta tttatctttt
     3961 tcgcctgtct tgccttgtcg ggagtgatta gaatgcgttc ttatctgccg gattaataca
     4021 agtgtttaag taaattattt tcgtaacgct tgcgtttaga aaaaaattta cgccacttgt
     4081 attaatccaa agatgagata agctctaaac acttctgaca aggtatgaca ggcgaaaaag
     4141 ataatgggcg taaggaatta taaatggcaa aaccaaataa agagcgatga atttcaatgc
     4201 tttcaggcgt agcttgaaag cctaaaagtc ttgaagaatg aaagactttt taccttcaaa
     4261 gcagacctag taatcttaaa taggcgatgg ctatttaaga ttacggtgtt gacccactaa
     4321 ataaacagaa atggaaccga tcaagcaaaa tcggactagg cactaggcga tggctaggac
     4381 ggtttgcggt ggctttagga aacgttcatg gtgagatgag catatctagc aatgtcgggc
     4441 taagctatgc ttagaacggc ttgcggtggc tttagaaaac ttttatggag agatgcacta
     4501 atctagcgat gtc
//
LOCUS       DAZKLD010000174         4347 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.177, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000174 DAZKLD010000000
VERSION     DAZKLD010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4347
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.177"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..387)
                     /locus_tag="ACG8KR_002685"
     CDS             complement(<1..387)
                     /locus_tag="ACG8KR_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0201994.1"
                     /translation="MKIKRWLPHLLFMFIIIIIIYPLLFIMANSFKPLKDAYQTITSL
                     IPQPFTLENFCYLFENLPIIKITRNTFFIATIITVVKLGVAFFAAYSFTYCRIKGKKI
                     WYFIIIGTMFIPFTVAMIPNYLLIAKL"
     gene            complement(377..1249)
                     /locus_tag="ACG8KR_002686"
     CDS             complement(377..1249)
                     /locus_tag="ACG8KR_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013669610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0201995.1"
                     /translation="MKRYVGYLYLAPAIILLGVFVFYPLIYTIYLSFFDWNMVAPTKE
                     FVGFANYVKVLTDPVFHKVLKNTLSYTVLFVLLSCVFPYITSFLLGIVIEKGQNLYKP
                     LFFIPAVISLVVAGMVFTWILNPVSGPIAVLLGNFGLRLPMWMNLQGWVIAVIAGITA
                     WKVFGYNFIVIFAAIVGIDQEMIEAAKLDRVPIWRIFFTIVLPMSSSTGLYLLVMTIV
                     QGLQYVFTPIKIITRGGPNNGSANVIYYAYQKAFEMYKVGESSAISVLTLLIFAVFLG
                     VIFKFVEKGVYYEN"
     gene            complement(1334..2623)
                     /locus_tag="ACG8KR_002687"
     CDS             complement(1334..2623)
                     /locus_tag="ACG8KR_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFQ0201996.1"
                     /translation="MLKKNLVIGVVSVLSVISLAACGSGNTKTTKKSEGRVTIEYWHV
                     NADTLGGKTVNELVDKYNQSQDKVKVVAKYNPDMYKGLMQNLQASVASKEIPDVVQVG
                     WAFKDYFAENFKYTNPQSLVEEVDPDNADYFKDHFLDNIMALSEKDGKQVGIPYSISN
                     PVLYLNQDILKAAGLDENGPKTWEEVQTFSKIIKEKTGKYGVYIQEPADNWAQQAILE
                     SNGASVIKDGKATFASKDGIAAYELYQRMVKDQTALHTTWEQGVQSFIDGNVAMVYTT
                     IAQRDNIQKNSTFTAKAVESPSWEGKKVKLPAGGAMLAVTSQTDKKKEATWDFLKYLY
                     SVESLAKWTEGTGYLPPRKDVADAQNGLKAFLAENQMMIPAIDQMDKMVSWSSFPGDA
                     GLEAEQKLLDMRDKILSGNSVNETMKATQKEINELLK"
     gene            complement(2767..3279)
                     /locus_tag="ACG8KR_002688"
     CDS             complement(2767..3279)
                     /locus_tag="ACG8KR_002688"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0201997.1"
                     /translation="MTINCDLKEEGLEMAVFSLAQTYQIWPQIVLSGYVQLSWAAIWG
                     KHIFYNIENQFEGKKLGDITEADLIDTLQKLAYQSVQIVNIHEGWLTKLAREVGQQLG
                     LTFSVWTVNELETIEYLARCGVYNITSRCAWAYLWKKGAQNKLFYNQNRGVKRFGDGI
                     HDHMGALFLA"
     gene            complement(3346..3495)
                     /locus_tag="ACG8KR_002689"
     CDS             complement(3346..3495)
                     /locus_tag="ACG8KR_002689"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFQ0201998.1"
                     /translation="MTMITAHSGSDNTEENSWAFLRFCLDRQLCFEVDVRQQTDGTLI
                     LAHDR"
     gene            complement(3972..>4347)
                     /locus_tag="ACG8KR_002690"
     CDS             complement(3972..>4347)
                     /locus_tag="ACG8KR_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFQ0201999.1"
                     /translation="ITRHVWGDLMDEVEHLRLTDLNKSIYKKRKQTIERIFADAKEKH
                     GMRWTKYRGLEKVATHTMLVFAAMNLKKLATWLWKGKEPLFFCSKIRNEVDKKLFQAR
                     VTSLEQLLSTVWLSVRTAFFVG"
BASE COUNT         1314 a          985 c          650 g         1398 t
ORIGIN      
        1 aagttttgca atcaacaaat aattaggaat catcgcaaca gtaaaaggaa tgaacatcgt
       61 accaatgatg ataaagtacc agattttctt ccctttgatt cggcaataag taaaactata
      121 tgccgcaaaa aaagctacgc ctaacttaac aaccgttata attgtagcaa taaaaaaagt
      181 atttctcgta attttgataa ttggaagatt ttcgaacaaa tagcaaaaat tttccaaggt
      241 aaacggctgt ggtatcaaac tagtaatcgt ctgataggca tctttcaatg gcttaaaaga
      301 attggccata ataaacaata atggatagat aataatgatt atgatgaaca taaataagag
      361 atgtggcaac cagcgtttaa ttttcatagt aaaccccctt ttccacaaat ttaaatatta
      421 cccctaagaa aacagcaaat attaataaag ttaaaactga aattgctgaa gattctccga
      481 ctttatacat ttcaaaagcc ttttgataag cataatagat cacgtttgca ctaccattat
      541 tcggtccacc acgagtgatg atcttaatcg gagtaaacac atactgcaga ccttgaacaa
      601 tggtcatcac caataaatat agtcctgtcg aactactcat aggcaacaca attgtaaaaa
      661 aaatgcgcca gatgggtaca cgatccaact tagctgcttc gatcatctct tgatcaattc
      721 ccacaattgc tgcaaaaatg acaatgaaat tataaccaaa aactttccaa gcagtaatac
      781 ctgcaatcac agcaattacc caaccttgta aattcatcca cataggcagt cttaaaccaa
      841 aatttcccaa caagacagca attggtcccg ataccggatt taatatccat gtaaaaacca
      901 tccccgccac taccaaagaa atcacagctg gaataaaaaa taatggttta tacaaattct
      961 gccctttttc tataactatt cctaataaaa aggatgtaat atatgggaac acacagctca
     1021 gcaagacaaa tagaacggta taggacaaag tattcttcaa cactttatga aagactggat
     1081 cagtcagcac ctttacatag ttggcaaagc caacaaattc tttagtgggg gccaccatat
     1141 tccagtcaaa aaaactcaaa tagatcgtat aaatcaatgg ataaaaaaca aacacgccca
     1201 acaaaataat tgctggcgcc agatacaaat aacctacata gcgcttcatt tccgacagcc
     1261 tcctcatcct tatgtcaaaa gcagacgacc ttgtttttcc tttcttacag gcgctgtcgt
     1321 ctgcttgctt tgcttatttg agtaactcat taatttcctt ttgagtagct ttcatcgttt
     1381 cattgacgga attacctgat aaaattttat cccgcatatc caacaatttt tgttcagctt
     1441 ctaatccagc atctccagga aatgaactcc aactgaccat cttatccatc tgatcaattg
     1501 ctggtatcat catctgattt tcagctaaga aagcttttaa tccattttgg gcgtctgcaa
     1561 catctttacg tggtggtaaa taaccagtac cttctgtcca tttagctaaa gattcaacac
     1621 tataaagata ttttaagaaa tcccaagttg cttccttttt cttatccgtt tgtgatgtaa
     1681 cagctaacat tgcgccacca gcaggtagtt taactttttt cccttcccaa cttggtgact
     1741 caactgcttt agcagtaaaa gtactatttt tttgaatgtt gtccctttga gcaatagtgg
     1801 tatagaccat cgcgacgtta ccatcaataa agctttgcac accttgttcc cacgtagtgt
     1861 gtaatgccgt ctggtctttt accatccttt gatataattc ataagcagcg ataccatctt
     1921 tagaagcaaa ggtagctttg ccatctttta ttacacttgc cccattactt tctaaaattg
     1981 cttgttgtgc ccaattatca gcaggttctt gaatatacac gccatacttt ccagtttttt
     2041 cttttattat cttagaaaaa gtttgaactt cttcccaagt ttttggacca ttttcatcca
     2101 agcccgctgc ttttaatata tcttgattta aatataaaac cggattacta atggagtaag
     2161 gaatacccac ttgtttacca tctttttcag acagtgccat gatattgtct aaaaaatgat
     2221 ctttaaaata atcagcatta tccggatcca cttcttctac taaagattga ggattagtat
     2281 atttgaaatt ttctgcaaaa taatctttaa aagcccatcc cacttgtact acgtccggta
     2341 tttctttgga ggcaacagaa gcttgtaaat tttgcatcaa gcctttatac atatcaggat
     2401 tatattttgc tacaaccttc actttgtctt gagattgatt gtatttatct actaattcat
     2461 tcactgtttt tccacctagg gtatccgcat taacatgcca atactctatc gttacacgac
     2521 cctccgattt ttttgttgtc ttggtattcc cactaccaca agctgccaaa gagatgacac
     2581 ttaaaacaga cacaacccct ataacaagat ttttctttaa cataacatta acttcctcct
     2641 aatttcttta tggccatttt tctaatcaag aaatattatg aactaccaat gtaaatttct
     2701 agttaatttt ctgtaaataa ttcgaaattt gtgcatttcg gggtacaggg tcactggaca
     2761 tatccatcaa gctaagaata aagcccccat atggtcatgt atcccatctc caaatctctt
     2821 aactcctctg ttttgattat aaaatagttt attctgtgct cctttcttcc ataaataggc
     2881 ccatgcacaa cgtgaggtta tattataaac accacagcgc gcaaggtact caatcgtttc
     2941 tagttcattg actgtccata cagaaaatgt caatcctagc tgctgcccca cttcacgggc
     3001 caacttagtt aaccatcctt catgaatatt gacgatttga acgctttgat aggctaattt
     3061 ctgtaaggta tcgatcaaat ctgcttcggt aatatcccca agttttttcc cttcgaactg
     3121 attttcaata ttataaaata tatgctttcc ccaaatcgca gcccaagata attgtacata
     3181 gccactaaga acaatttgtg gccagatttg ataggtttgt gccagcgaaa aaacggccat
     3241 ttccaatccc tcttctttta aatcacagtt aatggtcatg gttgccttct cattggccac
     3301 tgcttgaaac acctgctcaa gggttaccga atctgggaca acttcttacc tgtcatgggc
     3361 tagtattagc gtaccatctg tttgctgacg cacatctact tcaaaacaaa gctggcgatc
     3421 caaacaaaag cgaagaaatg cccagctatt ctcctccgta ttgtcactac cagagtgtgc
     3481 agtgatcatg gtcattttga cccctccttt aattccgtga ttttctccca cacgtccact
     3541 ataccgatga agcgttaatt tcttgttaat ttcttgtaaa ttccctgcgt tttttcagta
     3601 aactagttgt aaatcttgca tgctccttcc tacgaactca tgaatcgtcg attttgccct
     3661 tctagctagt ttcaccaatt aagtagttct acattccccg cttttaacct actacacctt
     3721 tttttgtttt cagctattcc ttcttcttgc tgaaagtcct tgaggttctt tcttacagga
     3781 taccatcaag gtgttcaatg gcttattccc attgaatgac cgagacttac tatcagattt
     3841 ctgctcgttg aaccaagtct ttgatacagg atttcttccg tcatcttctc acaaatacca
     3901 ccttgcgttt ctctagcgat tggtcgatgt gcaccctccc agtggactgt cactacccct
     3961 ttttgctatg cctagcccac aaaaaaagct gtcctcacag acagccagac tgtagacaaa
     4021 agctgttcca aactcgtaac tcgagcttgg aacagctttt tatctacttc gtttcgaatt
     4081 tttgaacaaa aaaataaagg ctccttgcct ttccatagcc aggtggctaa tttctttaaa
     4141 ttcattgcag caaacactag catcgtatgc gtcgcgactt tttccaagcc tcgatacttg
     4201 gtccaacgca tcccatgctt ttcttttgca tccgcaaata ttcgttcaat cgtttgtttc
     4261 cgttttttgt aaattgattt attcagatct gttaggcgaa gatgttccac ttcatccatc
     4321 agatctcccc aaacatgtct cgtgatg
//
LOCUS       DAZKLD010000175         4346 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.178, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000175 DAZKLD010000000
VERSION     DAZKLD010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4346)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4346)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4346
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.178"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            70..1116
                     /locus_tag="ACG8KR_002691"
     CDS             70..1116
                     /locus_tag="ACG8KR_002691"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFQ0202000.1"
                     /translation="MKVLLYFESEKMLAKSGIGRALDHQKRALTEVGISYTLDEKEDY
                     DILHINTYGINSHNMVNKARREGKKVVYHAHSTEEDFRNSFIGSNQLSPIVKKYLVGL
                     YQKADYLITPTPYSKQLLESYGIRVPIQAISNGIDLEKYHPDPMKEQKFREYFKLSPD
                     QKVIICVGLFFERKGIIDFVEIAKKMPEYTFIWFGHVPMYSIPRNIRKIVKEDHPENV
                     LFPGYIRGEIIEGAYSGADLFFFPSYEETEGIVVLEALASKQNVLVRDIPVYNGWLED
                     SKNCYMGKTNEEFIRLIQQITNQELPSTTQAGYQTAKERSINKIGEELKNVYESVLNE
                     KVSSKLKKVNQVKD"
     gene            1133..2356
                     /locus_tag="ACG8KR_002692"
     CDS             1133..2356
                     /locus_tag="ACG8KR_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010706866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFQ0202001.1"
                     /translation="MKIGFFTDTYFPQVSGVATSIKTLKQELERHGHEVYIFTTTDPN
                     ADKLEKDVIRMPSVPFISFKDRRVVVRGMWYAYLVAKELELDLIHTHTEFGAGILGKM
                     VAKKLKIPLIHTYHTMYEDYLHYIGKGKVIRQSHVKYLSRLFANHTTGVVCPSERVID
                     TLRSYGVIAPLRVIPTGIDIEKFKRSDITQQDISKLRESLGLQENHLMILSLSRISYE
                     KNIQALINGFPQVISAYPNARLVIVGKGPYVEELEELISELQLEEFVQFTGEVDNNDV
                     ALYYKAADYFVSASTSETQGLTYTEAMAAGTPCVVEGNAYLNRLFDHPSLGKTFETDE
                     DFAPALIDYISSEVPKDPKVLEAKLYDISSTHFGEAMIEFYQDMVVYHEEEMRRKEEE
                     DSIERIKIKLNSFKR"
     gene            2445..3332
                     /locus_tag="ACG8KR_002693"
     CDS             2445..3332
                     /locus_tag="ACG8KR_002693"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HFQ0202002.1"
                     /translation="MKLGFIGTGVMGSAVARHLLEAGHEVAVYNRTKAKADPLVTEGA
                     IWADTPKAVAEQSNILFTMVGYPKDVEEIYYGQSGIFSADISGHILVDLTTSTPSLAE
                     KIAKTAKEKGADALDAPVSGGDLGAKNGTLTIMVGGEEAVYDQVLPLFKEFGTTFTLH
                     GSAGKGQHTKMANQIMIAGTMTGMTEMLVYAQKNGLDLKKVIETLSGGSAANWSLSNY
                     SPRILKEDYTPGFFVKHFIKDLKIALEEAEKMDLVLPATTQALKLYEELADKGFENDG
                     TQALIKLWWPEGKIPEKKS"
     gene            3418..3648
                     /locus_tag="ACG8KR_002694"
     CDS             3418..3648
                     /locus_tag="ACG8KR_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HFQ0202003.1"
                     /translation="MTHSQLVAIIKRLEAMVESADNELQVRRFEKEGVEKCTVTYDKA
                     TDTFELTETDTHQQYEFDNIDIVAMEIYDLIQ"
     gene            complement(3705..>4346)
                     /locus_tag="ACG8KR_002695"
     CDS             complement(3705..>4346)
                     /locus_tag="ACG8KR_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide excision repair endonuclease"
                     /protein_id="HFQ0202004.1"
                     /translation="VLTDTPTARTYGPFRLYKKMPSILRIMEETYQMPWLSEISLLAL
                     RVQLPDLQEMTFEQKKKELQGLFQGRNKKLLTYLKKRQQHFIYQLNFEKAGMLQKDIE
                     LVTYFIRRIQEQKQFLRTPSLTFSMPLAADESQKKHYLVCYGQLAETMITSGDVSPDF
                     YYEKKEAHLSLKRQLSKEEIDPVQILISYRKKLEKEQFEIELLNKKEAEKQLN"
BASE COUNT         1474 a          725 c          902 g         1245 t
ORIGIN      
        1 aaatagggct taagtttgag tgacagtttg taggctatgt tatactatat aagtgaaggg
       61 ggacgtaagg tgaaggtatt attatatttt gaaagtgaaa agatgcttgc aaaatctgga
      121 atcgggcgtg cgcttgatca tcaaaaacgt gctctgacgg aagtcggtat ctcttataca
      181 ctagatgaaa aagaagatta tgacatcctg cacatcaata catacggaat caatagtcat
      241 aatatggtca ataaagccag aagagaagga aaaaaagttg tctatcatgc gcattctaca
      301 gaggaagatt tccgaaattc ctttatcgga tcgaaccagc tttctcctat cgtaaaaaaa
      361 tatctggtag gattgtacca aaaagcggac tatctgatca cgccaacacc ttattcaaaa
      421 cagttgttgg aaagctatgg tatccgtgtg ccgattcaag ctatttccaa tgggatcgac
      481 ttagagaaat atcaccctga tccaatgaaa gaacaaaagt tcagagaata tttcaaactg
      541 tctccggatc aaaaagtaat catttgtgtc ggtttgtttt tcgaacgtaa aggaatcatt
      601 gatttcgtag aaattgctaa gaagatgccc gaatacacat ttatttggtt tggtcatgtt
      661 cccatgtact cgattcctcg gaacatccgg aaaattgtaa aagaagatca tcctgaaaat
      721 gtcctgtttc ctggttacat tcgtggagag atcatagaag gtgcgtattc aggtgcagat
      781 ctattctttt tcccttctta tgaagagaca gaagggatcg ttgttttaga agctttagca
      841 agcaaacaaa acgtacttgt tcgagatatt ccagtgtata atggctggtt agaagatagc
      901 aagaactgct atatggggaa gacaaacgaa gaatttatcc gactgatcca acagattacc
      961 aatcaagaac taccgagtac gacacaagcc ggttatcaaa cagcaaaaga gcgaagtatc
     1021 aataaaatcg gtgaagaact taaaaatgta tacgaaagtg tactgaatga aaaagtctct
     1081 agcaaattaa aaaaagtaaa tcaggtcaag gactagtttg gaggcgaaaa acatgaaaat
     1141 cggctttttt accgatacct actttccgca agtcagcgga gttgctacat cgatcaaaac
     1201 attaaaacaa gaattagaaa gacatgggca tgaagtctat atttttacaa cgaccgatcc
     1261 taatgcggat aagttggaaa aagatgtgat tcgaatgcca agtgtaccat ttatttcttt
     1321 caaggatcga agagttgttg ttagaggcat gtggtatgct tatttggttg ccaaagaact
     1381 agaactagat cttattcata cccatacaga attcggagca gggatacttg ggaagatggt
     1441 agctaagaag ttgaaaatac ctctgatcca tacttaccat acgatgtatg aagactacct
     1501 tcattatatc ggtaaaggaa aagtgatcag gcaatcccat gtaaaatact tgtctagatt
     1561 atttgcaaat catacgacag gtgtagtttg tccaagtgaa cgtgttattg atacattacg
     1621 cagctatggt gtaatagcac cgcttcgcgt gattcctact ggtatcgaca ttgaaaaatt
     1681 caaacgttct gatatcactc agcaggatat cagcaaatta agggagtcac taggtcttca
     1741 ggaaaaccat ttgatgattc tttctttaag caggatttct tatgagaaaa atatccaagc
     1801 cttgattaat gggtttcctc aggtcatctc cgcttatcct aacgccagat tagttatcgt
     1861 aggaaaagga ccttatgtag aagagttaga ggaattgatc tcagaactgc agttagaaga
     1921 atttgtgcag ttcacaggag aagtggacaa caatgatgta gcactttatt ataaagcggc
     1981 agattatttt gtcagtgctt ctacttcaga aacacaaggg cttacttata cagaagcgat
     2041 ggcagccgga acaccttgtg tcgtggaagg aaatgcttat ttgaaccgat tgttcgatca
     2101 tccttcatta gggaaaactt ttgaaaccga cgaagacttt gcaccagcac ttattgatta
     2161 tatttccagt gaagttccta aagatccaaa agttttagaa gctaaacttt atgatatttc
     2221 atctacacat ttcggcgaag ctatgatcga attttatcaa gacatggttg tttatcatga
     2281 agaagagatg cgaagaaaag aagaagaaga ctcgattgaa cgaatcaaaa taaaattaaa
     2341 ttcatttaaa agataaaatt caaacatttt ttatgaaaaa atccttgttt ctagcgtata
     2401 ctagcataga agcaaggatt tttgattttt aggaggtcga aaaaatgaaa cttggtttta
     2461 tcggaacagg cgtcatggga agtgctgtcg cacgccatct actagaagca ggacacgaag
     2521 tagctgtcta taatcggaca aaagcaaaag ctgatccttt agtaacagaa ggagctatct
     2581 gggcagatac accaaaagcg gtagcagaac aaagtaatat tctctttaca atggtaggtt
     2641 accccaaaga tgtagaagaa atctactatg gacagtcggg gattttttct gcagatatct
     2701 caggacatat tttagttgat ttgactacaa gtacaccaag cctggcagaa aaaattgcaa
     2761 aaacagcaaa agaaaaaggc gcagatgcgt tagatgcacc ggtatccggt ggtgacttag
     2821 gagcaaagaa tggtacactg acgatcatgg tcggtggaga agaagccgtt tatgatcaag
     2881 tactgccttt attcaaagaa tttggtacga catttacatt gcatggttct gctgggaaag
     2941 gtcagcatac aaaaatggct aatcagatca tgattgcagg tacgatgaca ggaatgacgg
     3001 aaatgcttgt ctatgcacaa aaaaacggac ttgatttaaa gaaagtgatt gagacgcttt
     3061 caggtggaag tgcagctaac tggtctttaa gcaattattc tcctcgtatt ttaaaagaag
     3121 attacacacc tggttttttt gtcaaacatt ttatcaaaga tttaaagatt gcattagaag
     3181 aagcagaaaa aatggatctt gtattgccag caactaccca agcattgaaa ctatatgaag
     3241 agttggcaga taaaggtttt gagaatgacg gaacccaagc tttgatcaaa ctttggtggc
     3301 ctgaaggaaa aataccagaa aaaaaatcat aaaaaacgag tttttttaga gaaaatcaat
     3361 gcaagtaggc tatgcttttg atacaatata acagaaataa gaaaaggagg acctccgatg
     3421 acacattcac aattagtcgc aatcataaaa cgactggagg ctatggtcga gtcagcagat
     3481 aatgaactgc aagttcgccg ttttgaaaaa gaaggcgtag agaagtgtac agtaacttat
     3541 gataaagcga ccgatacatt tgaattaact gaaacagata ctcatcagca atacgaattt
     3601 gataatatcg atatcgtagc gatggaaatc tatgacttaa tccaataaat tttaggataa
     3661 ttgacatttg aagttgaaga tgcctaaaag aagccgagaa gcaactaatt tagttgcttt
     3721 tcagcttctt ttttgtttaa cagttcaatt tcaaactgtt ctttttctaa ttttttgcgg
     3781 taactgatca agatttgtac aggatcgatt tcttcttttg acaattgacg tttcaaagat
     3841 aaatgagctt ctttcttttc ataatagaag tcaggagaaa cgtcaccaga ggtaatcatt
     3901 gtttctgcaa gttgaccata acaaacgagg tagtgctttt tctgactctc atctgctgct
     3961 aatggcatgc tgaaggtgag cgatggtgtt cttaaaaact gcttttgttc ctggatccgt
     4021 ctaataaaat aagtaactaa ttcgatatct ttttgaagca taccggcttt ttcgaaattc
     4081 aactgataga tgaaatgttg ttgtcttttt ttcaagtaag tcagtagctt tttatttcgg
     4141 ccttgaaaca gcccttggag ttcctttttt ttctgttcaa aagtcatttc ctgtaggtca
     4201 ggaagctgga ctcgcaaagc taacaaacta atttcagaca accaaggcat ttgataagtt
     4261 tcttccatta tacgcaaaat agacggcatt tttttataga gacgaaaagg cccgtatgtt
     4321 cgagccgtag gagtatctgt taatac
//
LOCUS       DAZKLD010000176         4332 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.179, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000176 DAZKLD010000000
VERSION     DAZKLD010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4332
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.179"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..717)
                     /locus_tag="ACG8KR_002696"
     CDS             complement(<1..717)
                     /locus_tag="ACG8KR_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFQ0202005.1"
                     /translation="MSKKGTIINEWEKLTAPSNRRLSVPFMDTPLKIQWSIDRITIVG
                     NLKENIYYHTTHDVLILDFEQLMRLNEGNGYLRSVSNNGWQLLDQHEENIAYIEILKW
                     QEGKGRIDFNPNKISQFLASSMKNFIHDLFIEPHFSRADIACDIIDVPDEFITQYRVV
                     DPVSFKPIYGRNGKLETAYWGSRSSERQIRMYNKKLEQEKKRKIVPKEIVSWWRLELQ
                     LRRGKATDWHAMVQESLDSFA"
     gene            complement(1166..2572)
                     /locus_tag="ACG8KR_002697"
     CDS             complement(1166..2572)
                     /locus_tag="ACG8KR_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
                     /protein_id="HFQ0202006.1"
                     /translation="MGFSISFKKGTKNTSISHNNRELTDSQKINDWHKHIDFTKSDEN
                     IYLEQTNIREKYEELFGEAVEKYNAKQKRADRKIEDYLAKVRKDKKLEPQREFIVQIG
                     TLDDFRTTRDDGSSTGISEQQAEQNRVIANKILVEYFKEFKERNPNLSVYNAVIHNDE
                     VSPHLHLNIVPVAEGYKRGVQKQPSFNKALQQQGLRADKENGRVIWNNFRNQEVESIE
                     RLMADFGWERELVGTNKIKDVHEYKEIMSEISELRQTAVKERESVSDELRYISRQREE
                     NEREKALVASERVKIEEEKKNMLNVKKEMAVIFQKDIVPLESFKRLPDGSYHLSGNDF
                     VDLYQRATRATNAKRNADNMSEQYLQMLEQNTELNEYVATLEEERHLSGRKQLADLQV
                     ENKKLKEENRSLRQRLERLKNAFQKSITRLSIRFGLAEKDMGLSEELEVLEQEKTLFY
                     RQNVSQRKLERSEDEIEW"
     gene            complement(2793..3155)
                     /locus_tag="ACG8KR_002698"
     CDS             complement(2793..3155)
                     /locus_tag="ACG8KR_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0202007.1"
                     /translation="MGMTLADIKAQEEKLKKEQAKLRQQKKRLIEKENAAMGKALRKR
                     FGFETFADFERWLSSISDDQQHEKSVDNEWLADEKSAPSERDYPDESDGLFGVIPNKK
                     SQMSESKFTSIFNDVNLR"
     gene            complement(3159..3467)
                     /locus_tag="ACG8KR_002699"
     CDS             complement(3159..3467)
                     /locus_tag="ACG8KR_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0202008.1"
                     /translation="MDKIDLREETVDRIELMYERLADYLNLPDEDNYSEMLERIMDVL
                     NSVINADLAKSSVLTNEFVHKHLAQTTDESEKEFWLKLMSHVMQLNQISVINSKEKEM
                     "
     gene            complement(3476..3895)
                     /locus_tag="ACG8KR_002700"
     CDS             complement(3476..3895)
                     /locus_tag="ACG8KR_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0202009.1"
                     /translation="MTDLSEKMTIKNLADELGVSKNTVRNQLVNSGFDLAQYKENGII
                     MLDKELIGVVRSIYRSKSDQLTGQLPVNNTGSDFPLEKENEFLRDQLAEKDKHISELT
                     EMIRDQQKLTLLQQQKNDQLLLENQELKNRKWWQFWK"
     gene            complement(3888..4121)
                     /locus_tag="ACG8KR_002701"
     CDS             complement(3888..4121)
                     /locus_tag="ACG8KR_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0202010.1"
                     /translation="MTEKQKYFGKKNWLVNLTGDYVVVVFLDSKKMSGKLLDVTQYEL
                     ILKNNKIDSPIIIPKHAVKYIYAVAESSGEAND"
     gene            complement(4118..>4332)
                     /locus_tag="ACG8KR_002702"
     CDS             complement(4118..>4332)
                     /locus_tag="ACG8KR_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HFQ0202011.1"
                     /translation="MITALTTEHDYWGQINRKTKYKYRNLLKQESQNDELTNHLRETF
                     AESADDLKKELDTWLLGLDVTEEEEK"
BASE COUNT         1223 a          959 c          621 g         1529 t
ORIGIN      
        1 agcaaaacta tccaagcttt cttgaaccat tgcatgccaa tcagtcgctt tccctcgtct
       61 taattgtaat tctaaccgcc accaactcac gatttctttt ggcacaattt ttcttttctt
      121 ctcttgttcc agttttttat tatacatccg aatttgacgt tcagaagagc gagatcccca
      181 atatgccgtc tctaattttc cattccgtcc ataaataggc ttaaaagaaa ctggatcaac
      241 tacccgatat tgtgtaataa actcatcagg tacatcgata atgtcgcaag caatatctgc
      301 acgtgaaaag tgaggttcga taaacaaatc atgaataaag tttttcatgg aactagctaa
      361 gaattgacta atcttattcg gattgaagtc tatacgccct ttcccctctt gccattttaa
      421 aatttcaata taagcaatat tctcttcatg ttggtctaac aactgccaac cattattact
      481 aacagatctt aaataaccgt taccttcatt taggcgcata agctgttcaa aatctaaaat
      541 caacacatca tgtgtagtgt gataatagat attctcttta agatttccaa caatggtaat
      601 tcggtcgatt gaccactgaa tttttagggg ggtgtccata aaagggacac ttaaccgcct
      661 gttagagggg gctgttaatt tttcccactc atttatgatc gtgccttttt tactcaaatt
      721 ttgttcctcc tcaaagaaaa agagacttct ggaaagaatt ttcaaaagtt cacgtaactc
      781 ttaaaaattc attaccaaaa aatctctagt cttgaagaaa tctaaaaata ttcgtataat
      841 gattaagaag ttacggagct agtgcgatta gtttccgtaa aggtcgagaa cgtgtccagc
      901 gttgctcgac tttatttttt tgctcttttt tcagtttatt tatttgataa ctcaataata
      961 gcacttctct ttagctatgc aaatatccaa aaaataaaac cgcacccttt gaaaagggcg
     1021 cccccaaact tcgctaacgc taggttggcc aaaaaatgga cttgccattt tttagcaaat
     1081 gtcagcttca gggattagtg gtttagccgt caactcctta tcctcggcac aaaccgcctc
     1141 cggtaccgtt gactgcacat cgtaactacc actcgatctc atcttcagaa cgctctaatt
     1201 tacgctgtga gacgttttga cgataaaata aggttttctc ctgctctaac acttctaatt
     1261 cctcagaaag ccccatatcc ttttctgcaa gaccaaaacg gatagacaaa cgagtgatcg
     1321 atttttgaaa tgcgtttttt aatcgctcta aacgctggcg taaagagcga ttttcttctt
     1381 tcagcttttt attttccact tgtaaatctg ctaattgttt tctaccagac aaatgacgct
     1441 cttcttccaa tgtagctaca tactcattta attctgtatt ttgctcaagc atctgtaaat
     1501 attgctcact catgttatca gcatttctct tggcatttgt tgctctcgta gctcgttgat
     1561 acaaatcaac aaaatcattc ccagataaat gatacgaacc atcaggaagc ctcttgaaac
     1621 tttctaaagg aacaatatct ttttggaaaa tgaccgccat ctcttttttt acattcagca
     1681 tatttttctt ctcttcttca atcttcactc tctcagaagc caccagagcc ttttctcgct
     1741 cattttcttc tctttgccta gaaatatacc tcaactcatc agaaacgctc tcacgctctt
     1801 ttacggccgt ttgacgcaat tctgaaatct cgctcatgat ttctttatat tcgtgaacat
     1861 ctttgatttt gttagtaccg actaactcac gctcccaacc aaaatctgcc atcaaacgtt
     1921 caattgactc aacttcctga ttcctaaaat tattccaaat cactcgacca ttctctttat
     1981 ctgctcgtaa accctgctgt tgcaaggctt tgttaaaact tggttgcttt tgaacacccc
     2041 gtttataccc ttcagcaaca ggaacaatat tcaaatgcag gtgcggactt acttcgtcat
     2101 tatgaataac agcattgtag acggataaat taggattccg ttctttaaat tctttgaaat
     2161 attcaactaa aattttgttg gcaatcactc gattttgttc tgcttgttgt tccgatatgc
     2221 cagtagaaga accatcatca cgagtagtac gaaaatcatc aagagtacca atttgcacga
     2281 taaattctcg ctgtggttcc aattttttat ccttgcgaac ctttgctaaa tagtcctcga
     2341 tttttctatc ggctcttttc tgctttgcat tatatttttc tacggcttca ccgaataatt
     2401 cttcatattt ttcacgaata tttgtttgtt ctaaatagat attttcatcc gattttgtga
     2461 aatcaatatg cttatgccag tcattgattt tttggctatc agttaactca cgattattat
     2521 gagaaatgct tgtattcttt gttccctttt tgaacgaaat actgaacccc aagttaaaac
     2581 cctccttcgg ctgtcgctaa taaaaagagt gtaaaccttt ctgttactct ttgtcaaaga
     2641 gtaacatttt cctaattgtg acatggctgt cacaattcca aatgctctcc gagggtattc
     2701 gctgtcacaa gtgacaacga aaaaaggagc cgaaagcgtg tcacgctctc aactcctttt
     2761 acgctttatt ttgcttcacg caaaataatc gcttatcgta aattcacgtc attaaatatt
     2821 gacgtaaatt tactctcact catctgactt ttcttattcg gtatcactcc gaataagccg
     2881 tcagattcat cgggataatc tcgttcgctt ggcgctgatt tttcatcagc aagccactca
     2941 ttatccactg atttttcgtg ttgctgatcg tcagaaatcg acgatagcca ccgctcaaaa
     3001 tcagcaaaag tttcaaaccc aaaacgcttc cgaagagcct tgcccattgc agcattttct
     3061 ttctcaatca agcgtttttt ctgttggcga agtttcgctt gttctttttt taatttttct
     3121 tcttgcgctt taatatctgc taaagtcatt cccataaact acatctcctt ttctttggaa
     3181 ttaataacgg aaatttggtt taactgcata acatgactca ttaatttcaa ccaaaattct
     3241 ttttctgatt catcagttgt ttgcgctaaa tgcttatgaa caaattcatt agtcaaaact
     3301 gaagattttg ctaaatcagc attgataacc gagttgagaa catccataat tcgttcaagc
     3361 atttcactat aattatcttc atccggcaaa ttcaaataat cagctaaccg ttcatacatc
     3421 aattcaattc tatcgactgt ttcttctctc aaatcaattt tatccatgtc acacctcact
     3481 tccagaattg ccaccatttt cgatttttta attcttgatt ttctagtaac aactgatcgt
     3541 ttttttgttg ctgaagcagg gtcaattttt gttgatcacg aatcatttct gttaattcag
     3601 aaatatgttt gtccttctca gctaattgat cacgcaaaaa ctcattctct ttttctaaag
     3661 gaaaatcact gcctgtgtta ttgaccggta attgaccagt caattgatca ctttttgacc
     3721 gataaattga ccgaacaaca ccaatcaact ctttatctag catgataatg ccattttctt
     3781 tatactgagc aaggtcaaac cctgaattga ccagttgatt gcgcactgta tttttcgata
     3841 cacccaattc gtcagcaaga ttttttatgg tcatcttttc tgacaaatca gtcattagct
     3901 tctccactac tttcagctac cgcataaata tatttaacgg cgtgcttagg gataataatc
     3961 ggagagtcaa ttttattatt tttcaaaatc aattcatatt gcgttacatc aagtaactta
     4021 ccggacattt ttttgctatc caaaaaaaca accacaacgt aatcacctgt taaatttacc
     4081 aaccaatttt tcttaccaaa atacttttgt ttttctgtca tttttcttcc tcctctgtta
     4141 cgtctagccc tagaagccat gtgtctaatt ctttttttaa atcgtctgcc gattctgcaa
     4201 aggtttctcg taaatgattc gtcaattcat cattttgact ttcttgcttc agcaaatttc
     4261 gatatttata ttttgtcttt cgattaattt gcccccaata atcatgttct gttgttaaag
     4321 cagtaatcat ca
//
LOCUS       DAZKLD010000177         4316 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.180, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000177 DAZKLD010000000
VERSION     DAZKLD010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4316)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4316)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4316
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.180"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(212..442)
                     /locus_tag="ACG8KR_002703"
     CDS             complement(212..442)
                     /locus_tag="ACG8KR_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="resolvase"
                     /protein_id="HFQ0202012.1"
                     /translation="MDEAMDHPLLNKFIKDLILQIFSMIDEQEPTESKRHRAQGIIKI
                     AKRMFSIKGVLSYTVLIQKIPNHLVYKNNVED"
     gene            complement(672..1490)
                     /locus_tag="ACG8KR_002704"
     CDS             complement(672..1490)
                     /locus_tag="ACG8KR_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFQ0202013.1"
                     /translation="MSYIYLIKENYPFLTKSEKKVADFILNSGKTIIYSTMSDIKKNT
                     KVGDATIIRFCQKIGFSGFSDLKIEIAKEDFSQKKEHPTSVKYYDEIANSLTEALYST
                     IRLLDEEKLNEAIKMITQSKNIYIFGVGSSGNTSLDLENMFLRVGVQAKAVLDPHFQA
                     QVASLLTVNDLVIIFSLSGKTKDTYDSLKIAKKNGAKIIAITNYIHSPIGKSADLVLQ
                     TAIEEFLNGGSLAGKISQLYICDLLVHGYEQNNNIDSVELREKVLRSIIDKSIE"
     gene            1651..2340
                     /locus_tag="ACG8KR_002705"
     CDS             1651..2340
                     /locus_tag="ACG8KR_002705"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009385 - N-acylmannosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HFQ0202014.1"
                     /translation="MDKKRFLDLISKGLIVSCQALPGEPFYKEEESLMPYFALAAQRA
                     GAVGIRANSVRDIIEIRKCVSLPMIGIIKRDYPPESPFITATMREVDELVETGVEVIA
                     LDCTLRNRHDGLSINSFITKIKEKYPHQLFMADISTFEEGKNAYKAGIDFVGTTLSGY
                     TEESKKQDGPDIDLIKKLVEEGICVIAEGRIHTPEQAKEIKKIGVAGIVVGGAITRPQ
                     EIASRFVKALS"
     gene            2354..3856
                     /locus_tag="ACG8KR_002706"
     CDS             2354..3856
                     /locus_tag="ACG8KR_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFQ0202015.1"
                     /translation="MFKKFSQLGRAFMLPIAILPVAGLLLGLGGALTNESAINAYPFL
                     DQPWLHTILSIMSYAGNAVFANLALIFAIGIAVGLANGDKGTAGLAGGVAYLVYTATI
                     SGFLELFSAKDATLDTGVVGSIVVGGTVAFLHNRYRKIELPQFLGFFGGSRFIPIVSS
                     LAAIIIGSFFYLIWPPIQNGLVVAGEQIAQMGSLGTFLYGFLLRLTGAVGLHHTIYPL
                     FWYTSLGGTETVAGSTIAGAQNIFFAQLADPNHTGLFTYGTRFFAGRFATMMFGLPAA
                     CYAMYRAIPKKNRKKNGGLYFSGALTSFLTGITEPVEYMFLFVAPWLYVIHAFLDGLS
                     FYFADILNIRVGNSFSGGLIDFLLFGVLQGNDKTNWLKLIPFGIAWAFIYFFVFTFFI
                     KKFKVAIPGMEEESEVHSEKVINKNTSLHEESLIIIEALGGKENIESVEACATRLRVA
                     VTNGEIIQKQTIKDTGATAVFEVKGGIQAVFGGKADLLSQEINQILGKDN"
     gene            3869..>4316
                     /locus_tag="ACG8KR_002707"
     CDS             3869..>4316
                     /locus_tag="ACG8KR_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFQ0202016.1"
                     /translation="MFEIFKKKKQETFYAPCSGKIISITEVNDPVFSQKLLGDGFAVI
                     PDGSKIYAPLAGRVTNIFPTKHAINIKSNSGIDYLIHIGIDTVELQGEPFSICVQEND
                     EVTPETSLVDVDFDKIIKSGKDTSVIVVFPEKNQFNDLIIDNGRIAH"
BASE COUNT         1435 a          628 c          765 g         1488 t
ORIGIN      
        1 atatttactt cagatggtaa ggcaacaaaa taaaatacaa taggcagatg aactaataaa
       61 taaaatctgt attaaataca gataaattaa tataaaaatc aaaaggatca taaactttta
      121 acaagtgatg aaatgggtgg aaattgattc ttatagacag tttgtctcat aacattgaag
      181 tattttgcaa ttttagcatt gaaacacttt tttaatcttc aacgttgttc ttataaacaa
      241 gatgattggg aatcttttga attaacactg tatagcttag aacacccttt atagaaaaca
      301 ttcgcttagc aatcttaata attccttgtg cccgatgtcg tttagattca gtaggttcct
      361 gctcatcgat catggaaaaa atttgaagga tcagatcctt aataaattta tttaagagag
      421 gatgatccat tgcttcatcc atactcgata aactagtgat aatgagctac acgtttttct
      481 ttcaagttga tcaatagctt ccacgataaa aatatcttta gataaaatca agaaccgtag
      541 gaactacagg gatatttggt caataagagc caccgctatt agtaaaaaat aagctatcaa
      601 gtatgctctc atcccaagaa ttgcctactt caatcgttaa aaaccgttag gtttagctaa
      661 gtgggggtag ttcactcaat acttttatca ataatagagc gcaaaacttt ttctcttaat
      721 tcaactgaat caatattatt gttttgttca taaccatgaa ctaatagatc acaaatataa
      781 agttgtgata tttttccagc taaagatcca ccattaagaa attcttcaat ggctgtttgt
      841 aaaactaagt ctgcagactt tcctataggt gaatgaatat agttagttat agcaataatt
      901 tttgcgccat tttttttagc tattttcaat gaatcataag tatctttcgt tttcccagat
      961 aaagaaaaaa tgattactaa atcattaact gttaataaag aagcgacttg ggcttgaaaa
     1021 tgagggtcta gtacagcttt tgcttgaact cctacgcgaa gaaacatatt ttctaaatca
     1081 agactagtat ttccactaga tcccactcca aatatatata tgttttttga ttgagtgatc
     1141 attttaatgg cttcattcaa tttttcttcg tctaaaagcc taatagtgga atagagagct
     1201 tctgttaaac tattagcaat ttcatcatag tactttacac tagtcggatg ttcttttttt
     1261 tgactaaagt cttcctttgc aatttcaatt tttaaatcag aaaaaccaga aaaaccaatt
     1321 ttttgacaaa agcgaataat agtagcatcg ccaacttttg tgtttttctt aatatcactc
     1381 atcgtactat aaataatagt tttcccagaa tttaaaataa aatcagcgac ttttttttca
     1441 gacttcgtta aaaatggata attttcttta attaaataga tataactcat acaaactcct
     1501 ctacaatttc aacttttctc cattatactc caaaataata aaaaaaactc ctaaatactc
     1561 catttttatc tttactttta tgaaagcgga tgctatattg tttttggagt tgttatccaa
     1621 aaataataaa aaattggagt gtttaggata atggataaaa agaggttttt agatcttatt
     1681 tcaaaaggtt tgattgtttc ctgtcaagca ttacctggtg aaccatttta caaagaggaa
     1741 gaaagtttaa tgccttattt tgctttggct gctcaaaggg ctggagcagt tggtatacgt
     1801 gcaaattctg ttcgagatat tattgaaatt agaaagtgtg tatcattacc aatgattgga
     1861 attattaaaa gagattatcc accagaatct ccattcatta ctgccactat gagagaagta
     1921 gatgaattag tagaaaccgg tgttgaggta atcgctcttg attgcactct tagaaatcga
     1981 catgatggac tatctataaa ttcttttatt actaaaataa aagaaaaata tcctcatcag
     2041 ttatttatgg cagatatttc tacatttgaa gaaggcaaaa atgcatataa agcaggaata
     2101 gattttgtag gaactacttt aagtggatat actgaggaaa gtaaaaaaca agatgggcca
     2161 gatatagatt tgattaaaaa attggtagaa gaagggattt gtgtaattgc tgagggaagg
     2221 attcatactc cagaacaagc aaaagagata aaaaaaatag gagttgcagg tattgttgtg
     2281 ggtggtgcta ttactcgacc acaggaaatt gcatcaagat ttgttaaagc attaagttag
     2341 ggggagaaat aaaatgttta aaaaattttc acaattagga cgcgctttca tgttaccaat
     2401 tgctatatta ccagtagccg gattactatt gggactggga ggagcgttaa caaatgagag
     2461 tgctataaat gcttatccat ttttagatca accgtggttg catacaattt taagtattat
     2521 gagttatgct ggaaatgcag tttttgcaaa cctagcacta atttttgcaa ttggtattgc
     2581 tgtaggactt gcaaatggag ataaaggaac agctggttta gctggtggag tagcttatct
     2641 agtatatact gcaactatta gtgggtttct tgaacttttt tcagcaaaag atgcaacact
     2701 agatacaggt gtagtgggat caattgttgt tggtggtact gttgcatttt tacacaatcg
     2761 ttatagaaaa attgaattac cacaattctt aggttttttt ggaggatcaa gatttattcc
     2821 gattgtttct agtttagctg caatcattat tggttctttc ttttatttaa tttggccacc
     2881 aattcaaaat ggtttagtag ttgctggtga acaaattgcc caaatgggta gtttaggaac
     2941 ttttttatat ggttttttat tgcgtttaac aggtgctgtt ggactacacc acaccattta
     3001 ccctttgttc tggtatacat ctttaggtgg taccgaaact gtagctggat caacaattgc
     3061 tggagcccaa aatattttct ttgcccaact agctgatcca aaccacactg gtctgtttac
     3121 ttatggtact agattttttg caggaagatt tgcaacaatg atgtttggat taccagcagc
     3181 atgttatgca atgtatcgtg caattcctaa gaaaaatcgt aaaaagaatg gtgggcttta
     3241 ttttagcggg gcccttacat catttttaac tggaattact gaaccagttg aatatatgtt
     3301 tttatttgtt gctccttggc tttatgtgat tcatgcattc ttagatggat tatcttttta
     3361 ttttgctgat atcttaaata ttcgtgtagg gaattccttt tcaggtggtt tgatcgactt
     3421 tttattattc ggtgtacttc aagggaatga caaaactaat tggttaaaat taattccttt
     3481 tggtattgca tgggcattta tatatttctt tgtttttaca ttctttatca aaaaatttaa
     3541 ggtagctatt cctggaatgg aagaagaatc agaagttcat tctgaaaaag taataaacaa
     3601 aaatacatca ttacatgaag agtctcttat cattattgaa gctcttggtg gaaaagaaaa
     3661 tattgagtct gttgaagctt gtgcaactcg tttgcgtgtt gctgttacga atggggagat
     3721 tattcaaaaa caaacaataa aagacactgg tgcaactgct gtcttcgaag tgaagggtgg
     3781 catccaagct gtttttggag ggaaagctga cttattaagt caagaaatca atcaaatatt
     3841 aggaaaagat aattaggagg tttttaatat gtttgaaatt ttcaaaaaga aaaaacaaga
     3901 aactttttat gcaccttgtt ccggaaaaat catttccatc acagaagtaa atgatcctgt
     3961 cttttcacaa aaattactag gtgatggttt tgccgtcata cctgatggtt caaaaattta
     4021 tgctccattg gctggtcgag taacaaatat atttcctact aaacatgcta ttaatataaa
     4081 aagcaactca ggcattgatt atttgattca tattggaatt gatacagttg aattacaagg
     4141 agagcctttt tcgatttgtg ttcaagaaaa tgatgaggtt actcctgaaa cctcgttggt
     4201 tgatgtggat tttgacaaaa taataaaatc tggtaaagat acttctgtta tagttgtttt
     4261 tccggagaaa aatcagttca atgatttaat aatagataat ggtcgaattg ctcatg
//
LOCUS       DAZKLD010000178         4190 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.181, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000178 DAZKLD010000000
VERSION     DAZKLD010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4190)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4190)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4190
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.181"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..2885)
                     /gene="brxC"
                     /locus_tag="ACG8KR_002708"
     CDS             complement(<1..2885)
                     /gene="brxC"
                     /locus_tag="ACG8KR_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BREX system P-loop protein BrxC"
                     /protein_id="HFQ0202017.1"
                     /translation="MLIKDIFAKPIDRNIQGVIKVGQAKDENVQQELEEYVVTKELQK
                     HFKTIFDAYQRSINTPTDKMGVWIQGFFGSGKSHFLKIISYLLANREINDKKAIDYFK
                     EDGKIKDPLTLATMERCSQINTDVILFNIDSKSDAGGKKDKNGIVNVFLKVFNEMQGL
                     STIPHLADFERRLIKEEKFDAFKAAFEEINGAKWQEERHEFDWIQDDIKEAALKIDFM
                     SEAAIDNFLEKASGNYLISIDDFANLVKSYLDSKEADHHIAFLVDEVGQYIGGNRELT
                     LQLQTVVEDLGRICEGKAWVLVTSQEAIDHVTNFDNNSDNDFSKIKDRFATTVSLSSA
                     NADEVIKERILKKKEFANDSLALQFETVQTELKNKLLFVDSPELKLFQDAENYAEVYP
                     FIPYQFNLLGQILTEIRNHSYQGSNLSSGERSLLAMFKEAAERNKENDTTSLVSLDQF
                     YYSLERWIDTAISRVITQATNNSHIQRPTDIDNFNVNVLRILFMIKYVDQAIKPNVEN
                     ITSLMIRNINEDRLETKKRVEEALDLLIKENFVRKNLDSYIFQTDEEQEITREIKNQN
                     ITDSDLIVELSKRIFDGVLDRNSFAYEKLGMSGGNKFKNRYTFTYAQVLDQRPYKQAG
                     NAEMTVNFISPYSDLAGQEAELALKSSRNLLFVELPSDKEFKDDIAMYARMNLYLTTT
                     YSSVVKNFDAIRSEKSGELQELSASINDQLNELLKSAKFFYNGAEIQLTGSDFKAKFQ
                     SALQKMAEEVYHKLNQIDAPKEKEDILYLVKESKNEIIEDPSNEQAVQEIFNFINDQK
                     RNHLTITMKTIRDRFETKQPYGFTELDVEWCIAKLFMSGKIDLLYNNVQISRMDDPNS
                     IVMIIQKRRESEKIKIEPSAVISPKAKKLVDEIGRELFQSRNIVDENNNEHTMENFKK
                     YLKGQLDNLRSYFDLQNTYTYPSAYEYEQIKKYLEVIVQTSDVEDFF"
     gene            complement(2910..3485)
                     /locus_tag="ACG8KR_002709"
     CDS             complement(2910..3485)
                     /locus_tag="ACG8KR_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1788 domain-containing protein"
                     /protein_id="HFQ0202018.1"
                     /translation="MSLSIQEHLEKLNEVIQKEDFLLNRGLGNEVGYYIFDYDPKDEL
                     IVRDYINDISGTSVGDNYEVKVYNLYVIMLEYLEKRGFTEKAIKMEERRGLQKLVESI
                     GRLLKMDLPEEENVFFEYVDAHTPEKSVVFLTGIGELYPLIRAHSILNKMHQVFDRVP
                     VVMMYPGEYDELSLTILNTNKDDNYYRAFRI"
     gene            complement(3485..4084)
                     /locus_tag="ACG8KR_002710"
     CDS             complement(3485..4084)
                     /locus_tag="ACG8KR_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1819 family protein"
                     /protein_id="HFQ0202019.1"
                     /translation="MNKKYSAGLVSKRFWFSEFKLYIQLRLEGYTDIEIKEKNEHENI
                     FFAVSSNRQKEIYQNVKRRVSQLDEAGLQLFNQLNIDNQKLVNLISILMIDDLFNEFM
                     TEVYAENLRKGNLQLTPVDYRSFFTEKRRSSEDISKWKDYTIKRLIAAYQTNLIETGL
                     VRGEKEYGRMTPILLDKRLIQWLVDKNRKDIVLALGGIL"
BASE COUNT         1259 a          796 c          567 g         1568 t
ORIGIN      
        1 taaaaaaagt cttcaacatc tgacgtttga acaattactt caagatattt ttttatttgc
       61 tcatattcat aagcactagg gtaggtataa gtgttttgta aatcaaaata agagcgtaaa
      121 ttgtctaatt gaccttttaa atatttcttg aaattttcca ttgtatgttc attgttattt
      181 tcatcaacaa tattacgact ttgaaacagt tctctaccga tttcgtcaac caattttttt
      241 gcttttggcg aaatcaccgc gcttggttca atttttatct tttcactttc tcgtcgtttt
      301 tgaataatca ttacaatact atttggatca tccatccgtg aaatttgtac attgttataa
      361 agtaaatcaa ttttaccaga catgaaaagt ttagcgatgc accattccac atcaagttct
      421 gtgaatccat acggttgttt tgtttcaaaa cgatcacgaa ttgttttcat cgtaatcgtt
      481 aggtgatttc tcttttgatc attgataaaa ttgaaaattt cctgaactgc ttgctcattt
      541 gatggatctt caataatttc atttttgctt tctttcacta aatataagat atcttctttt
      601 tctttaggcg catcaatttg gtttaattta tggtatactt cttcagccat tttctgaagt
      661 gctgattgga attttgcttt aaagtcggag cctgtcagtt gaatttctgc accgttatag
      721 aaaaattttg cagatttcaa cagttcattt aattgatcgt tgatacttgc acttagttcc
      781 tgtaattcac cagatttttc cgaacgaatc gcgtcaaagt tctttactac actcgaataa
      841 gtcgttgtta aataaagatt cattctagca tacattgcta tatcatcttt aaattcttta
      901 tctgatggca attctacgaa taataagtta cgggaactct taagcgcaag ttctgcctct
      961 tgacctgcta aatcagagta tggtgaaata aaattaactg tcatttcagc atttccagct
     1021 tgcttgtatg gacgttgatc taacacttgt gcataagtaa acgtataccg gtttttaaat
     1081 ttatttccac ctgacatgcc taatttttca taggcaaaac tatttcggtc tagaacgccg
     1141 tcaaaaattc gtttagatag ttctacgatc aaatcactat ctgtaatatt ttgattttta
     1201 atctcacgag taatttcctg ttcttcatca gtttggaaaa tataactatc taagttcttc
     1261 cgcacaaagt tttccttaat taaaaggtcc aatgcttctt ctactcgttt tttagtttcc
     1321 aggcggtctt cgttgatatt tcttatcatc aagctcgtaa tattttcaac atttggtttg
     1381 atggcttgat caacatattt aatcatgaat aaaattcgta agacatttac gttaaagtta
     1441 tctatatcag ttggtctttg aatatgagaa ttatttgtag cttgtgtaat cactcggcta
     1501 attgctgtat caatccaacg ttctaacgag taataaaact gatctaaaga aaccaaactg
     1561 gttgtatcat tttccttatt tcgttcagcg gcttctttaa acattgctag taatgaacgt
     1621 tcaccagacg ataagtttga tccttgatag ctgtgattac gaatctcggt tagaatttgc
     1681 cccaataaat tgaattgata gggaataaat ggataaactt ctgcgtaatt ttcagcatct
     1741 tggaagagct ttaattcagg actatcaaca aagagtaatt tatttttcaa ttctgtttga
     1801 acagtttcaa attgtaaggc taaagaatca ttggcaaact cttttttctt caaaatacgt
     1861 tctttaatta cttcatcggc atttgctgaa gataaactca cagttgtggc aaaacgatcc
     1921 ttgatcttag aaaaatcatt atcagaattg ttatcaaagt ttgttacgtg atcaatcgct
     1981 tcctgacttg tcactaatac ccaagcctta ccttcacaaa ttcgacccaa atcttccaca
     2041 acggtttgta actgcaaagt caactcacga ttaccgccga tatattgtcc gacctcatcg
     2101 actagaaagg caatatgatg gtctgcttct ttggaatcaa gatagctttt aactagattt
     2161 gcaaagtcat caatgcttat taaataattt cctgatgctt tttctaaaaa attatcaata
     2221 gcggcttcac tcataaaatc gattttaagt gccgcttctt tgatatcatc ttggatccaa
     2281 tcaaactcat ggcgctcttc ttgccatttt gccccattaa tctcttcaaa agcagctttg
     2341 aatgcatcaa acttttcttc tttaattaac cgacgttcaa aatctgctag atgtggaatc
     2401 gtgcttaatc cttgcatttc gttaaatacc tttaaaaaaa cattcacgat tccgtttttg
     2461 tcttttttac cacctgcatc actttttgaa tcaatattaa acagaataac atctgtattt
     2521 atttgtgagc accgttccat cgttgccaaa gtcagtggat ctttgatttt cccatcttct
     2581 ttgaagtaat caatggcttt cttgtcattt atttctcgat ttgcgagcaa gtaagaaata
     2641 atttttaaga aatgagattt acctgaacca aagaacccct gaatccaaac acccatttta
     2701 tctgttggtg tattgatact ccgttggtag gcatcaaaaa ttgttttaaa gtgtttttgt
     2761 agttctttcg taaccacgta ttcttctaat tcttgttgaa cattttcatc ctttgcttga
     2821 ccgaccttaa taactccttg aatatttcgg tcaattggtt ttgcaaaaat atctttaatt
     2881 agcatttaat ctcctacctt ccatcccaac taaattctaa atgctcgata ataattatca
     2941 tctttattgg tattaagaat tgtcaaactt aattcatcat attcccctgg atacatcatc
     3001 acaactggta cgcgatcaaa tacctgatgc attttattta aaatagagtg cgctctaatt
     3061 aatggatata attcgccaat tcctgtcagg aacacaactg atttttctgg tgtatgggca
     3121 tccacatatt caaaaaatac attttcttcc tcaggtaaat ccattttcaa taaacgacca
     3181 atgctttcca ctaatttttg taaaccacgt cgttcttcca tcttaatcgc tttttcagtg
     3241 aaaccgcgtt tttctagata ctctaacata atcacataga gattataaac tttcacttca
     3301 taattatcac caacagatgt tccagatata tcattaatat aatctcttac aattaactcg
     3361 tctttcggat cgtagtcaaa gatataatag cctacttcat tacctaaacc acgattcaat
     3421 aaaaaatctt ctttttgaat tacttcattc aacttttcca aatgctcttg aatcgataat
     3481 gacattataa aattcctccc agtgctagta caatatcttt acgattcttg tcaactaacc
     3541 attgaatcaa tcgtttatct aataatatcg gagtcattcg tccatattct ttttcccctc
     3601 tgactaatcc tgtttcaatt agattcgttt gataagcggc aataagccgt ttaatcgtat
     3661 aatctttcca ttttgaaata tcttctgaac ttctccgctt ctctgtaaaa aaagaacggt
     3721 aatcaactgg tgttaattgc aaatttcctt tacgtagatt ttcagcatat acctcagtca
     3781 taaattcatt aaaaagatca tcaatcataa ggatggaaat caaattcact aacttttgat
     3841 tatctatatt taactgattg aaaagttgca atccagcttc atccagttga ctaactctcc
     3901 ttttcacgtt ctgatagatt tctttttgac ggttactgct cactgcaaaa aaaatatttt
     3961 catgttcatt tttctcttta atttctatat ctgtatatcc ttctagtcgt aattgaatat
     4021 ataacttgaa ctctgaaaac caaaagcgtt tagaaactag accagcagaa tattttttat
     4081 tcaatagatt gcctcctaat catctataat tcaataagta aacaagtgtt ttttgctcat
     4141 cgttagcatt cattttttag tatctactat acaatacata tttattatag
//
LOCUS       DAZKLD010000179         4186 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.182, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000179 DAZKLD010000000
VERSION     DAZKLD010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4186)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4186)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4186
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.182"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            31..732
                     /locus_tag="ACG8KR_002711"
     CDS             31..732
                     /locus_tag="ACG8KR_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFQ0202020.1"
                     /translation="MRVNKIDFHFSKLRYQPDFEHFSDEEFQQIKEHTVLRSYKKGQI
                     LFDDGDPRQRLYILTKGLVRLERYDESATYYYYDYVNTNNLFPMSGMFGEETYTYTAQ
                     AMTDIETFYLPVSYYEKLARKNPEQLIYMIQKISKVVEIHELRIQIGLTSSAFDRVKQ
                     NLFILKEELGVPTKDGHITIPYPITLKELAVNSGTTRETAGQVIKQLRDSGLLDYDKK
                     RFTFCKTATTPAMRE"
     gene            complement(1304..2320)
                     /gene="tsaD"
                     /locus_tag="ACG8KR_002712"
     CDS             complement(1304..2320)
                     /gene="tsaD"
                     /locus_tag="ACG8KR_002712"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HFQ0202021.1"
                     /translation="MNEELILAIESSCDETSVAVVRNGTEILSNIVASQINSHKRFGG
                     VVPEVASRHHVEQITLCLEDALVEAGVSAEDLSAVAVTYGPGLVGSLLIGISAAKAFA
                     WAHQLPLIPVNHMAGHIYAARLVKPFQFPLMALLVSGGHTELVYMQEDGSYEIIGETR
                     DDAAGEAYDKVGRVLGLSYPSGKEIDQLAHQGKDNYHFPRAMIHEDNYDFSFSGLKSA
                     FINLVHNAQQRGEDLDKNDLAASFQASVIDVLITKTLRACQNYPVKQLIVAGGVAANQ
                     GLREGLQDALATKLPEVELVIPPLRLCGDNAAMIGAAAHVEMQKRNFASYQLNADPSL
                     VLSE"
     gene            complement(2317..2781)
                     /gene="rimI"
                     /locus_tag="ACG8KR_002713"
     CDS             complement(2317..2781)
                     /gene="rimI"
                     /locus_tag="ACG8KR_002713"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HFQ0202022.1"
                     /translation="MICRKKDFDEAYLAECVWMISENAYPHGSPWTKMQFLADIQQPH
                     SDYLLLVEDNQICGFIGYSKVIDEVEITNIAVAVSEQGKGHARHLLQLLITEQIKDSL
                     HVFLEVRLSNEAARKLYESEKFRILGKRKSYYRNPTEDAIIMSRKLKTETMK"
     gene            complement(2788..3330)
                     /gene="rimI"
                     /locus_tag="ACG8KR_002714"
     CDS             complement(2788..3330)
                     /gene="rimI"
                     /locus_tag="ACG8KR_002714"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HFQ0202023.1"
                     /translation="MLKKFRAYARSFFQDKMIYTEKTIEQIDRRYVLRELTTEDIKEL
                     LDVEREVYNGELPWTKSAFISELKSPLPHLYLGIFDNGRMIGFIGSRAFGLDCHITNI
                     AVRPAYQRKRIGSLLIDEIENFAIMNRCETMSLEVRMSNQDAQRLYRKLGFQATTIKK
                     RYYTETKEDALDMIKLLENE"
     gene            complement(3314..4036)
                     /gene="tsaB"
                     /locus_tag="ACG8KR_002715"
     CDS             complement(3314..4036)
                     /gene="tsaB"
                     /locus_tag="ACG8KR_002715"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HFQ0202024.1"
                     /translation="MITLGIDTANQTLAIGVVEDEQILGQIQTNIKRNHSVTLMPAID
                     QLFADLQISPKDIDRIAVSDGPGSYTGLRIGVTTAKTIAYTLDKELVGVSSLKTIAAN
                     CIGVDKIIVPMFDARRKNVYAGAYRWKENRLEIVLEDVHISASDLFTQLQKLNEAIYF
                     VGSDCHKFFYEIKEILPSAMINTVPAWDIPSGVTVAQLGALAEPVAEKQAFLPRYLKR
                     VEAEEKWLETHTPGDESYVEKI"
BASE COUNT         1234 a          896 c          728 g         1328 t
ORIGIN      
        1 tatagtgaac aataaggaga ggacttttta atgcgtgtta ataaaatcga ttttcacttt
       61 tcaaaactca gatatcaacc tgattttgaa catttctcgg atgaagagtt ccaacagatc
      121 aaggaacata ctgttcttcg ttcttataaa aaaggacaga tccttttcga cgatggtgat
      181 cccagacaac ggttgtatat cttgaccaaa ggactcgttc gtttagaacg ttatgatgaa
      241 agtgcaactt attactacta tgactatgtg aatacaaata acctttttcc tatgtcagga
      301 atgtttggag aagagaccta cacttataca gctcaagcga tgactgatat tgaaacattc
      361 taccttcctg tttcctacta tgaaaaatta gcacgaaaaa atcccgaaca gttgatctat
      421 atgatacaaa aaatttctaa agtagttgaa atacatgaat tgcgtatcca aatcggattg
      481 acctctagcg cctttgaccg ggtaaaacaa aatttattca ttttgaaaga agaattgggc
      541 gtaccaacaa aagatggtca tatcaccatt ccttatccga tcaccttaaa agaattagca
      601 gtgaatagcg gcacgacacg tgaaactgct ggacaagtaa tcaaacaact acgtgattcc
      661 ggactgttag attatgacaa aaagcgtttt actttctgta agacagctac tactccagct
      721 atgagagaat aacgcgaaca aaaataagca gtgaccctga tatttatcag ggtcactgct
      781 tattttcctc cctaaaactt ctgaaagatc cggcagtttt agatcactgg gcaagactca
      841 aagattttct atgtgtgttt cagttatgta atattcactg tttgcaaaaa atactgatag
      901 attaactaga ggaagcgtct tgtcctcatc cgacctagat tattcgatat tcctcgacaa
      961 taaacgatta atcagtgagg gcaatcagag gaacggataa tgatgaataa gctgtaaaaa
     1021 atagggcttt gacaaaagcg tgtcacagcc cttatttcga ttaaacggtg tgtacctgtc
     1081 aactcttcgg tcacaagtca taatgttcat aaacatgagg agctgttttt tgaaattgtt
     1141 ccttgtgaat cagagttaaa cgacaggctt acgaactctt gattaaacgg cgttcaaaag
     1201 cttgcatacg cggtaataag aacagccagg tcaaaaacat gagaagccgt ttttcctggg
     1261 ctgttcttat tacttgatgc agggcagatt tttcacaacc tctttattca gataaaacca
     1321 agcttggatc agcattcaac tgatagctag caaaatttct tttttgcatt tccacatgtg
     1381 ctgctgctcc aatcattgca gcattatctc cacaaagtcg aagaggggga atcactagtt
     1441 ctacctcagg gagtttagtt gccaaagcat cctgcaatcc ttcacgcaac ccttgattgg
     1501 ccgctacacc tccagctaca atcagttgct tgactggata attttgacaa gctcgaagtg
     1561 ttttagtaat caatacatcg atcacacttg cttgaaagct agctgctaaa tcatttttgt
     1621 ctagatcttc tccacgctgt tgtgcattat gcaccaaatt gataaatgca ctttttaatc
     1681 cgctaaaact gaaatcataa ttatcttcat ggatcatcgc tcgtggaaaa tggtaattat
     1741 cttttccctg atgagctagc tgatcgattt cttttccgct tgggtaagaa agacctaata
     1801 ctcttccaac tttgtcataa gcttctcctg ctgcgtcatc tcttgtctcc ccaatgattt
     1861 catatgatcc atcttcttgc atatagacta attctgtatg tcctccgcta acaagaagtg
     1921 ccatcaatgg aaactggaat ggtttaacca gacgggctgc atagatatgc cctgccatat
     1981 ggtttacagg aataagcggc aattggtgtg cccaagcaaa agccttagct gcagaaatac
     2041 caatcagcaa cgagccgaca agtcctggtc cataagtgac agcgactgcc gataaatctt
     2101 ctgccgacac accagcttct accagtgcat cttccaagca taaggtgatt tgttctacgt
     2161 gatggcggct agctacttca ggaactacgc caccaaaacg tttatgactg tttatttgag
     2221 aagcaacgat gttagataag atctctgttc catttctaac gaccgctaca ctcgtttcat
     2281 cacaacttga ttcaatcgcc agaatcaatt cttcattcat ttcatcgtct ccgtttttaa
     2341 ctttctgctc ataatgatcg catcttccgt cggattgcga taatatgatt tccgtttacc
     2401 taatatacga aacttttctg attcataaag ttttcttgct gcttcattag atagacgtac
     2461 ttctaaaaaa acatgcagcg aatcttttat ttgttccgta atcagtaatt gtaacaaatg
     2521 ccgtgcatgc ccttttcctt gttctgaaac tgcaacagca atatttgtga tctcgacttc
     2581 atctattact ttcgagtatc caataaagcc acagatctgg ttatcttcaa ctaaaagcag
     2641 ataatcagag tgaggctgtt ggatgtctgc caaaaactgc attttcgtcc aaggtgaacc
     2701 atgtggataa gcattttcgc taatcatcca tacgcattct gccaagtacg cttcatcaaa
     2761 atcttttttt ctgcaaatca actaatctca ctcattttct aaaagtttta tcatatccaa
     2821 cgcatcttct tttgtttcag tgtaatatct ttttttgatc gttgttgctt gaaaacctaa
     2881 cttccgataa agtctttgtg catcttgatt actcatacgc acttctaatg acatcgtctc
     2941 acagcgattc attatagcaa agttctcgat ttcgtcaata agcagcgaac cgattcgttt
     3001 acgctgatat gccggtcgaa cggcaatatt cgtgatatgg caatctaaac cgaacgctct
     3061 tgaaccgata aaaccaatca ttcttccatt atcaaagata cctagatata gatgaggaag
     3121 cggtgacttc aattctgata taaaggcact ttttgtccat ggcaattcgc cattatagac
     3181 ttctcgttcg acatccagca gctctttgat atcttctgta gtcaattctc ttagtacgta
     3241 acgacgatcg atttgttcga ttgttttttc tgtataaatc attttatctt gaaaaaagct
     3301 tctggcatac gctctaaatt ttttcaacat aactttcgtc tccaggtgtg tgcgtctcca
     3361 accatttttc ttctgcttct acccgtttta aatagcgagg aagaaaagct tgtttctctg
     3421 caactggttc agctaaagca cctaattgag ccacagttac accacttgga atatcccatg
     3481 ctggaactgt attaatcatc gcactaggca atatttcctt tatttcatag aagaatttgt
     3541 gacaatcact cccgacgaaa taaatagctt cattcagctt ttgtagttgt gtgaacaaat
     3601 cagaagcaga aatatgtaca tcttcaagta caatttctaa tctattttct ttccagcggt
     3661 atgccccagc gtatacattt tttcttcgag catcaaacat gggcacaata attttatcca
     3721 caccgatgca attagccgcg atggttttca gactagatac tcctactagc tctttatcta
     3781 atgtataagc aatcgttttt gctgtcgtta cacctatacg aagccctgta taagaaccgg
     3841 gaccatcaga aaccgcgatt cggtcaatgt cttttggact tatttgaaga tctgcaaaca
     3901 attggtcgat tgcaggcatc agtgttacac tgtggttgcg tttgatattt gtttgtattt
     3961 gtccaagaat ttgttcgtct tctaccacac cgatcgccaa agtttggttt gcagtatcta
     4021 ttcctaatgt aatcatatat ttgatccttt ctgctatgac attctcgcct tccattgtag
     4081 catacttttt tattctgagg atgaaaatac caaaggcaaa actagtacaa tgatacatga
     4141 ttatctgctg gcgggcaaat tttgcccctt cctgaaacac aaaaaa
//
LOCUS       DAZKLD010000180         4182 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.183, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000180 DAZKLD010000000
VERSION     DAZKLD010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4182)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4182)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4182
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.183"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..418
                     /locus_tag="ACG8KR_002716"
     CDS             <1..418
                     /locus_tag="ACG8KR_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0202025.1"
                     /translation="LLFLEVIHVYGFVFLTNKKAGSTMDHLYIKYIHQFSVLVLGTLI
                     IAGIVAFIIQGRDILTVHQEFFSYAFGIPLIAIRSDNKKWKKRYYILLLFLCFYLPPK
                     IFTLLGIQCLSFSFISYIIVLIFLSFIVWVFYLVKK"
     gene            513..1397
                     /locus_tag="ACG8KR_002717"
     CDS             513..1397
                     /locus_tag="ACG8KR_002717"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFQ0202026.1"
                     /translation="MKKVLLVVNPSSGGEQAKEFEQLAIAKLESVFDEVVVLHTKKAG
                     DAKNFTREAATEGYHSVFVMGGDGTVNEGISGIAEQEHRPNFGFFPLGTVNDLARALG
                     IPLEPEEAINHFSIESVKPLDIGKINDDYFMNVVAIGSIPEAINDVDAEKKTKFGKLA
                     YFMSGIKQLASTQSYSFHVEVDGKKEEIESSILLIGLTNSVGGFETLLPNAKVDDGKL
                     HLVYLKDSSLIDTLKTLPDLLKGVDESTKNLAYLTCEQLVVSLADEEELTTNVDGDEG
                     DKLPVTIKVLPSHLQVYC"
     gene            1619..>4182
                     /locus_tag="ACG8KR_002718"
     CDS             1619..>4182
                     /locus_tag="ACG8KR_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HFQ0202027.1"
                     /translation="MNQYVKKLIASILFVFPLLMVTSVFADVEKTSESTQSENQSFDK
                     TTFSEESITSETTESQSEAPTSNTRQEENKTSETSEVAEQTSDSSEEVQVRVVSADGY
                     SEAATTEELTQLLADESVAKIRLTQPLTLDRELEIKRDIVIDFGGLAHNFGTHHIYIN
                     ETPQSIEFQNFKGTAAHPGGLIPNIDGNAIIIAYMSDFWGNRYHFTGEIKFTGTLDLY
                     DGSKLGLIYAPRATVTLDGVSGVLDVQPVKEGMNAAPGKAYFCRSYQLNVINGSQLYG
                     PYLGKFYGFLDSGDETGSGKTAPGLHILSGSKVSLDYDRADGVSDEGEAVDTLPGNVT
                     FKVSGSGSEFSVNTKINITNDNNRGIIQMRGSGSRVAVSNDGKITINTATTGGFRLQG
                     DSSQIHVSSGGQISVKMAGDGDQPGNNGMRFVGKGLSLIVEGAGSVIDIDKQSGRSSA
                     VRFENGTQKLTVTNGGSLKVRNAGDGKSYVNRLNQGNQAIQFYDASGFLESPGSADFT
                     VTGEKSNIDIRADSGATVDTTGDIDLNFMAGEKTYLVMVGKTGEDKTGIISGDVLNVD
                     LESPTYFDFQNKRIGSETNGIGGWVFQGNGDSSLKIAHSEIALWRKAGVTFDNFEKDP
                     DYFSDMTDLSINGTDLGNFVSSPSAGLTNEFAQTGAGMKNYNRISANNQLPMIESLRV
                     PTNADKKIYGHVVVPEGVEAKPRDAWTGEVEVTLRITYADTTKSPVEITALTQGQTDE
                     NEGTGYNPWGEGEQGGLFEVQVPNEERLTTGDQVQIVAAKKVRGGQEVTELDSAKTTV
                     DVIPPEPAKLSTTLINAASRSITGTGEAGALVSLKKNQAVLSETTIDDTGKFVLSIP"
BASE COUNT         1360 a          709 c          946 g         1167 t
ORIGIN      
        1 tcttttattt ctagaagtta ttcacgtata cggatttgtt tttctcacaa ataaaaaggc
       61 aggaagtaca atggatcatt tgtatataaa atatattcat caattttccg ttttggtatt
      121 aggcacacta attatagcgg ggattgttgc atttattatt caaggtcgtg acatattaac
      181 agtacatcaa gagtttttca gttatgcatt tggtattcct ctgatcgcta taaggtctga
      241 taacaaaaag tggaagaaac gctattacat tctcttgcta tttttgtgtt tttatctgcc
      301 acctaagatt tttactttat taggtataca atgcctatca ttttctttta tttcctatat
      361 cattgtttta attttcctca gtttcatagt atgggtattt tatcttgtca aaaaataaat
      421 gaatgtttca tgaacagtac ttcatcacag cctaattttt ttctttatgc tatcgttata
      481 ttaaagaaat caatcagaag ggaaagaaag tgatgaaaaa agttttatta gtggtaaacc
      541 ccagttcagg cggtgaacaa gcaaaggaat ttgagcaatt agcgatagca aaattggaat
      601 cggtgtttga tgaagtggtc gtcttgcata caaaaaaagc aggggatgca aaaaacttta
      661 ctcgcgaagc ggctacggaa gggtatcata gtgtgtttgt catgggcgga gatggaacag
      721 tcaacgaagg aatcagtgga atcgctgaac aagaacatcg gccaaatttc ggatttttcc
      781 cattgggtac ggtgaatgac ttggctcgag cattagggat accgctagaa cctgaggaag
      841 cgatcaatca tttttcgatt gaatcagtca agccattgga tattggaaaa ataaatgacg
      901 actattttat gaatgtcgtc gcaattggct ctatccctga agcaatcaat gatgtagatg
      961 cggagaagaa gacgaaattt ggaaaactag cttatttcat gtcaggaatc aaacagttag
     1021 cttccacaca atcctactcg tttcacgtag aagttgatgg aaagaaggaa gaaattgaga
     1081 gcagtatctt gctgatagga ctaactaact cagtaggcgg ttttgaaaca ttacttccaa
     1141 atgcaaaagt ggatgatggt aagctccatt tggtttattt aaaagattct tctttgatcg
     1201 atacgcttaa aacattgcca gacctattaa aaggagtaga tgaatctacg aaaaatctag
     1261 cctatcttac ttgtgagcaa ttagttgtgt cattagctga tgaggaagaa ttgacgacga
     1321 atgttgatgg agatgaaggg gataaattac ctgtgacaat caaagtgtta ccctcacatt
     1381 tacaggttta ttgttaaaat ggaggttgtg accgaagagt tgtcaggagg acatcggttg
     1441 ttcggaataa gggactgtga caagactttt gtcacagcct ttttttgtac aaaaaagaaa
     1501 ttttcaccta acagaaaaat ttgccataaa aatacatatt ttaaatatga aagtacaatt
     1561 acaaataaat taaaacgtta aaataaaatt gtcaaaacgt cagagaggat ggtggagaat
     1621 gaatcaatat gtaaaaaagc tgattgcgtc gattttgttt gtcttccctc tcttgatggt
     1681 gacatccgtt tttgccgatg tagagaaaac aagcgaatca acgcaatcag agaatcaaag
     1741 ttttgacaag actacttttt cagaagaatc aattacttct gaaactacag aatcccaaag
     1801 tgaggcgcct acttctaata caaggcaaga agaaaataag acaagtgaga cctcagaagt
     1861 agctgaacaa acgtctgata gcagtgaaga ggtacaggtt agagttgttt cagcggatgg
     1921 gtacagcgaa gctgccacaa cagaagaact gacgcagctt ctcgcagacg aatcagtagc
     1981 taagattcgt ttaactcagc ctttgacttt agacagggaa ttggagatca agcgagatat
     2041 cgttatcgat tttggcggac ttgctcataa tttcggcaca catcacatct atatcaatga
     2101 gacacctcag tcgatcgagt ttcagaattt taaaggaacg gcagctcatc caggtgggtt
     2161 gataccaaat atcgatggaa atgccattat cattgcttat atgtccgatt tttggggaaa
     2221 cagatatcat tttacgggag aaataaaatt taccgggaca cttgacttat atgatggttc
     2281 taaattaggc ctgatttacg cccctcgtgc aactgtcaca ttagatggtg tttccggtgt
     2341 attggatgtc caaccagtga aagaagggat gaatgccgct ccgggtaaag cttatttttg
     2401 tagaagctat cagctaaatg taatcaatgg tagtcagctt tatggaccat atctaggaaa
     2461 attctatggt tttctagatt caggtgatga gacgggcagt ggaaaaacag caccaggact
     2521 ccatatcttg agtggttcaa aggtctctct tgactatgat cgggcagatg gcgtcagcga
     2581 tgaaggagag gctgtcgata cgctacccgg gaatgtcaca tttaaagtca gtggctcagg
     2641 aagtgaattt tcggtaaata caaagatcaa tattaccaac gacaacaatc gcggaattat
     2701 ccaaatgaga ggctcaggat caagagtcgc tgtgtcgaat gatgggaaaa tcacaatcaa
     2761 tacagcgact actggcggat ttcgcctgca aggagattct agtcaaatcc atgtatcaag
     2821 tggtggacaa atctcggtca aaatggctgg agatggcgac caacctggaa acaatgggat
     2881 gcgttttgta gggaaaggac tttctttgat cgtagaagga gccggatcag tcatcgatat
     2941 tgataagcaa agtggtcgtt ctagcgcagt tcgttttgag aacggtacgc aaaaactaac
     3001 agtaactaat ggaggctcat tgaaggtaag aaatgcaggg gatggcaaat cttatgtgaa
     3061 cagactcaat caaggaaacc aagcgatcca attttatgac gcttctggtt ttctagaaag
     3121 tccagggtca gctgatttta cggttactgg agaaaaatca aatatcgata tacgcgcaga
     3181 ttccggagct accgtagata caactggaga tattgatttg aactttatgg ctggcgaaaa
     3241 aacttattta gtcatggtcg ggaaaactgg ggaagataag actggcatca tcagtgggga
     3301 tgtgttgaat gtagacttag aatctcctac ctatttcgat ttccaaaaca aacggatcgg
     3361 aagcgagact aatggcatag gtggctgggt atttcaagga aatggcgatt cttctttgaa
     3421 gatcgctcat tcagaaattg ctttatggcg aaaggcagga gtaacgttcg ataactttga
     3481 aaaagatccg gattattttt ctgatatgac agacttatcg atcaatggaa ccgacttagg
     3541 taattttgta tcaagcccta gtgcaggttt aacaaatgaa tttgctcaga caggtgccgg
     3601 aatgaaaaat tataatcgaa tcagtgccaa taaccagctt ccgatgattg aatccttaag
     3661 agtaccaacg aatgcagata aaaaaatcta tgggcatgtc gttgttcccg aaggtgtcga
     3721 ggcaaagcca agagatgctt ggacaggcga agtagaagtg actttacgta ttacttatgc
     3781 agatacgacg aaatcacctg tagaaattac tgctctcaca caaggacaaa cagatgaaaa
     3841 tgaagggaca ggatataatc cgtggggaga aggcgaacaa ggcggtttgt ttgaggttca
     3901 agtacccaac gaagaaagac taacaacggg ggatcaagtg caaatagttg ctgctaaaaa
     3961 agtaagaggc ggccaagaag tgacagaact ggactctgcc aaaacgacag tcgatgtgat
     4021 tcctcctgag cctgctaaat taagtaccac attgatcaat gcagcttctc gctcaatcac
     4081 aggtacaggc gaagcaggag ctttggtttc attgaaaaaa aatcaagctg ttttgtccga
     4141 aacaactatt gatgatacag gtaaatttgt tctttctatc cc
//
LOCUS       DAZKLD010000181         4152 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.184, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000181 DAZKLD010000000
VERSION     DAZKLD010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4152)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4152)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4152
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.184"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            417..1385
                     /locus_tag="ACG8KR_002719"
     CDS             417..1385
                     /locus_tag="ACG8KR_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFQ0202028.1"
                     /translation="MDVAKTVNAIVEKNDGEISGIYLVACGGSLVDMYVSDYFLKAEA
                     SKCITGLYTANEFNHVTPKQLDDKSVVILCSHGGNTAETVKAGEVAQARGAVTVGLTH
                     NKTADLLKVSDHSFLYEWGDDAKIVNNPMAIILDLTVSLVNVTETFEHYGKFREGMKV
                     IDTVIENGIKQVQDRIKVFADKYKDETMFYILGSGPSYGHAYGFSICSLMEMQWLDST
                     SVHSGEFFHGPFEVTDKDTNFILLMSIGRTRALDERAKRFLDKYAERVEIVDAKELGL
                     DMISDEVSEFFNPILFYSILSEYRSALADARNHDLDVRRYMGKVEY"
     gene            1396..2211
                     /locus_tag="ACG8KR_002720"
     CDS             1396..2211
                     /locus_tag="ACG8KR_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HFQ0202029.1"
                     /translation="MKVLGLGDNVVDKYENLGIMYPGGNSLNFAVFAKKLGHESSFMG
                     VFGSDEEAAHVLSAIKKIGLDNSHSRFEKGENGCARVKIDEGDRIFLGSNSGGVTREY
                     PIQLTEEDREYLNQFELIHMGLYSHVNHLLEELQNVSGKISYDFSDDFTEEEVQRAID
                     KVDFGFFSIEDFSDEEVKQFLMKHFCERNDVLIATRGEKTAIGYDGAKFYEVNPVLEE
                     PVDTMAAGDSFLTAFLLYFLEGDDIVTAMKKGNEFAGKSCLVDGSFGFGIHYD"
     gene            2279..2989
                     /locus_tag="ACG8KR_002721"
     CDS             2279..2989
                     /locus_tag="ACG8KR_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFQ0202030.1"
                     /translation="MLDTNAQLPLYLQLKEILKNKINTGEYPENTKIPAESELIESYE
                     VSRITVRKAVEELVEEGYLLKKQGKGTFVNRHKVFRKIEYVMGFSESCKSNGFTPTSI
                     VLERKIIPATEGMAKKLDIAQGDDVLYIQRKRMADNIPIMLENNYFSYERYSFLMEEE
                     LTDSLYEVLKVQGTLAINPGETALEIVTADDQIAKIMEVAVGTPFFFMDTLINDQNNI
                     PIHVGNQYYLGEVYRFYL"
     gene            3025..>4152
                     /locus_tag="ACG8KR_002722"
     CDS             3025..>4152
                     /locus_tag="ACG8KR_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HFQ0202031.1"
                     /translation="MKQVLRSKMIFDGEQPPFKGYIVINEGIIEKVENNWNYSDLLNE
                     NTELIAYDDQFVMPAIHDNHVFFSGYLAMNAGIDLSDTSSANEAVERIKAVMNHKKAD
                     EPIYAHGWDAALWKTDPSEELLNEINHFGPITAIDKNRSHCWMNAIAKQTYGFTEKET
                     SAESRVLLIKEMLANKKLLAEVYAEFEALLLSKGVISMKEIVFDDAEFLSNIPQRKIL
                     SNLYVQAVQEPLQHELLYEYKEKVFPEKVRFGGVKIMVDGVVADRSGDIYGVYQSGVA
                     SPEIDYEAIKAEVAAFNEVEIPCCLTTEGDKAGYKAASILVEFGKRLPEGVFNSISDL
                     EMVTQKTAEKMNEGQVVAEVYPQILGLNQTLEEAYMSTVIKN"
BASE COUNT         1388 a          625 c          884 g         1255 t
ORIGIN      
        1 actaaaagaa attctgcaat tcaaaataaa gcattttaag ccattttcaa atttaagtgc
       61 acccatctac ccgtcaaatc actttatttt ccactccagt agtttcctaa ttcaactgtg
      121 tgcaaaacga tttcttctaa acaaatcaag acaaacttat aaaccttcat cccagcacca
      181 taattattta aatcataggc atttttcgaa attagtatat gtactcaagt ttcactgcag
      241 attatttata ttttttagag aaataaattt ttcctattga caaacacaaa gaaagcggat
      301 acaatacaat tatgtcacat gacgttatat gacgttatgt aatatcttta catttaatca
      361 gttactttta ttttggataa aattaacatt tttaaaattt taaggaggaa atgttgatgg
      421 atgtagcaaa aacggttaac gcaattgtag aaaaaaatga tggagagatt tctggaattt
      481 atttggtggc ttgtggcggt tcgctggtag atatgtatgt ctcagattat tttcttaaag
      541 ccgaagcatc gaagtgtatt acaggcttat atacagcgaa cgaatttaac catgtaaccc
      601 caaagcaatt agatgataaa tctgttgtca ttctatgttc tcatggagga aatacagcag
      661 aaacggtaaa agcaggagaa gtcgctcaag ctagaggagc tgtgactgtt ggtctgactc
      721 ataataaaac tgcggattta ttaaaggttt ctgatcatag tttcctttac gaatggggag
      781 atgatgcaaa aatagtcaat aatcctatgg ccatcattct tgatttaact gtatctttgg
      841 tcaatgttac cgaaacattt gaacattacg gtaaattcag agaaggcatg aaggttatcg
      901 ataccgtaat tgaaaatggc attaagcagg tgcaggatcg tattaaagtg tttgctgata
      961 agtacaaaga tgaaacaatg ttttatatct taggtagtgg accatcatat ggacatgctt
     1021 atggtttttc aatttgctct ttgatggaaa tgcaatggct tgattcaact tctgttcatt
     1081 ctggagaatt cttccatgga ccttttgaag taacagacaa agatacgaat tttattctct
     1141 tgatgagcat tggacgcact cgtgctttag atgaacgggc aaaacgattc cttgataaat
     1201 atgcagaaag agtagaaatc gttgatgcaa aggaactagg attggatatg atatcagatg
     1261 aagtttctga attcttcaat cccattttat tctacagtat tctttcagaa tatcgttcag
     1321 ctttagcaga tgcacgcaat catgatttag atgttcgtcg ctatatgggg aaagtggagt
     1381 actaggagga ttaaaatgaa ggtattaggc ttaggtgata acgtcgtcga taaatatgaa
     1441 aatctgggaa ttatgtatcc tggaggaaat tcattgaatt ttgcagtttt tgcaaaaaaa
     1501 ctgggacatg aaagtagctt tatgggagtt tttggctcag atgaggaggc agctcatgtt
     1561 ttatcagcaa taaaaaaaat aggactggac aacagtcact ctcgctttga aaaaggcgag
     1621 aatggctgtg ccagagtgaa gattgatgag ggcgaccgga ttttccttgg cagcaattcg
     1681 ggaggtgtta ctcgtgaata cccgattcag cttactgaag aagacagaga atatttaaac
     1741 cagtttgaat tgattcatat gggattatat agccatgtca atcatctatt agaagagtta
     1801 caaaatgttt ctggaaagat ttcttatgat ttttctgacg actttacgga agaagaggtt
     1861 cagcgggcaa ttgataaagt tgattttggc ttcttttcta tagaagactt ttccgatgaa
     1921 gaagtaaagc agtttttgat gaagcatttt tgtgagcgaa atgatgtatt gattgcaacc
     1981 cgaggagaaa aaacggctat tggttatgat ggagcaaagt tttatgaagt gaaccccgtg
     2041 ctagaagaac cagttgatac aatggcagcg ggagattctt tcttaacagc tttcctactt
     2101 tattttctcg aaggggacga tatagtcacc gcgatgaaaa aggggaatga atttgctggg
     2161 aagtcttgtc tggttgacgg ttcgttcggc tttggtattc attacgattg agcttgtttt
     2221 aaattcctgc atttgataac ctaaagaaag aaatcccggt aaagtggagg ttaaaaaaat
     2281 gctggataca aatgcacaat taccattgta cttacaatta aaagaaatat taaaaaataa
     2341 aatcaataca ggggagtatc ccgagaatac gaaaattccc gctgaatcag agctgataga
     2401 aagttatgag gtcagccgaa tcacagtgag aaaagcagta gaagaactgg tagaagaagg
     2461 gtacctactt aaaaaacaag ggaaaggaac ttttgtcaac cggcataaag tttttcgtaa
     2521 aattgaatat gttatgggtt tcagtgagag ttgtaaatct aatggtttta caccgactag
     2581 cattgttttg gaacgcaaga tcattccagc aactgaaggg atggcaaaaa aactcgatat
     2641 cgctcaaggt gatgatgttt tatatatcca gagaaagcga atggcggata acatccccat
     2701 tatgttagaa aacaattatt tttcttatga aagatatagt ttcttaatgg aggaagagct
     2761 gactgattct ttgtatgaag tacttaaagt ccaaggtacc ttagccatca atcctgggga
     2821 aactgcactt gaaattgtca cagctgatga ccaaattgcc aaaataatgg aagtagctgt
     2881 tgggacgccc tttttcttta tggatacttt aatcaacgat caaaacaaca taccaattca
     2941 tgtaggtaat caatattacc tgggagaagt atatcgtttt tacttatgat ttcctgtttt
     3001 gatttcaagg agtagatttt atttatgaag caagtattgc ggtcaaaaat gatttttgat
     3061 ggagagcagc cacctttcaa aggttatatt gtaatcaatg aagggatcat tgaaaaggtg
     3121 gaaaacaact ggaattacag tgatctatta aatgaaaaca cggaattaat tgcttatgat
     3181 gatcaattcg ttatgccagc aattcatgat aatcatgtat ttttcagtgg ttatttggct
     3241 atgaatgctg gcatagattt aagcgataca tcttcagcta acgaggctgt agagcgtatt
     3301 aaagctgtaa tgaaccataa aaaagcagat gaaccaattt atgctcatgg ctgggatgct
     3361 gccctgtgga aaactgaccc atcagaagag ctattgaatg aaataaacca ctttggtcca
     3421 atcacagcga tcgacaaaaa tcgcagccat tgttggatga atgctattgc taagcagact
     3481 tatggtttta cagaaaaaga aaccagtgct gaaagtcgag tattattaat taaggaaatg
     3541 ctagcaaaca aaaagctttt agcagaggtt tacgctgaat ttgaagcatt actgctttca
     3601 aaaggtgtta tatcaatgaa agaaatcgtt tttgatgacg cagaatttct ttctaatata
     3661 cctcagagga aaatacttag taacctatat gtgcaggcag tccaagaacc tctacagcat
     3721 gaacttctat atgagtataa ggaaaaagtt ttccctgaaa aggtccgctt tggtggagtg
     3781 aagatcatgg tagatggtgt tgttgcggat agaagtggag atatttacgg tgtttatcag
     3841 tcaggagtag cttcacccga gattgactat gaggcaatta aagcagaagt tgctgcattc
     3901 aatgaagtag agattccttg ctgtttgaca acggaaggag ataaagcggg ttataaagct
     3961 gcgtcaattc ttgtcgaatt tggaaagcgg ttacctgaag gcgtttttaa ttcgattagt
     4021 gatttggaaa tggttactca aaagacagca gaaaaaatga acgaaggaca agtagtagca
     4081 gaggtatatc cacaaattct cggcttgaat caaacgttag aagaggctta catgtcaact
     4141 gttataaaaa at
//
LOCUS       DAZKLD010000182         4101 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.185, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000182 DAZKLD010000000
VERSION     DAZKLD010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4101)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4101)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4101
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.185"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..575)
                     /locus_tag="ACG8KR_002723"
     CDS             complement(<1..575)
                     /locus_tag="ACG8KR_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0202032.1"
                     /translation="MTESVYVHIDTTSNAVLTKGLTATDFANSIVHFPQNLLLLDPSA
                     SAGEYESHTGLKVIRGTENIQRFFSSSRNRRGFDELKWIDFTDLTMLKELTPLEISEL
                     LYFGHMKTHLHSPFFYKLQNNFVYFDLNDQLNRIYYRYLDEFYRIFASKLSSILSDKI
                     NVKKSFFKKETPVEKISVELLKEMRNLMQEG"
     gene            842..2542
                     /locus_tag="ACG8KR_002724"
     CDS             842..2542
                     /locus_tag="ACG8KR_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HFQ0202033.1"
                     /translation="MEIKKVYFSQKMSLYSPIQQAKQPLPFFQSHQESRDAFWRSAKK
                     QCAFKAVSKKYTVYFTIAKSRGDVIFNNLLIEGETFEWKKFFRYMEACARFFIKENCC
                     FLFQSPLSEEWLRIFHKNGYQGTTQLNKKLVYHTALVLGGGGAHGAYQIGVWQALKEH
                     DINFEIITGTSVGALNGALILQGDMKKAVNLWKKLSTRQVLALPEMAAVEDLRERFYR
                     ERRQMTKTALIEGGVSSAPLEKMVKDNLDLSLLKHNPKIRLYTVSTKLPELAEVVTDI
                     QQTKTEEIPDWILASASFYPAMAYRKIGKNKYADGGYRNKIPIDIAINKGATEAFVVD
                     VQGPGPAKKIRMPDIFIHWKCQTLWTLGSFLLFDSQRNQLNLQLGYLEMKKRLGYYYG
                     NWYTFHSVKPARTYWRGFLSYLTKEIQLELSFFKSIKFWQKLRNLYKNRVVPETCGLA
                     MLELLAKKHFLLPNEIYQVDMMIQMICQQDIKSMPDDLLAQIGQLSTEEWRRYRKYQQ
                     KIQNERTKTVYFDYLLQGKRKDRLQHELLKQPVDTLLILYLYYLKEEQPWHKNFHMKS
                     "
     gene            2512..2730
                     /locus_tag="ACG8KR_002725"
     CDS             2512..2730
                     /locus_tag="ACG8KR_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbC family protein"
                     /protein_id="HFQ0202034.1"
                     /translation="MAQEFSYEIVEEIAILSENNKGWRKELNLVSWNGRPPKFDLRDW
                     APDHEKMGKGLTLTNEEFEQLQKAIENM"
     gene            2750..3640
                     /locus_tag="ACG8KR_002726"
     CDS             2750..3640
                     /locus_tag="ACG8KR_002726"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HFQ0202035.1"
                     /translation="MKSMTGFGKAIRETKAYQLDIEIKSVNQRFLDVQIRSPKILNFV
                     ENDFRQLIKQQLSRGRIEVFVNLAYIGKNEKQIVINWELIDSLITQLTDGIQQRYGSQ
                     NQVSISRMAERFALDENFVTVEEKPEESMEELAQLALDTLKSALDSIEASREKEGQAL
                     ALVLKKNTEEFKEVLKKLDSFVEIYEKEFQIRYQKKLEEFLGAKVDEQRLLTELAILL
                     ERGDIHEELDRLAIHVQKLDELIEAQCPVGRELDFLIQEMNREINTIGSKSNAIEIKN
                     QVVQSKTILEKIREQVQNIE"
     gene            3658..3942
                     /locus_tag="ACG8KR_002727"
     CDS             3658..3942
                     /locus_tag="ACG8KR_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0202036.1"
                     /translation="MKYYDVTFHELSGKTVVKRDIPSEKNGFDVWKDACADYNENELF
                     ILINDGAYVTMNRKFIVRIDTEEVEDPTEKARSRKDEIMGVVNTLSNMGF"
BASE COUNT         1433 a          682 c          816 g         1170 t
ORIGIN      
        1 atcccttcct gcatcaaatt acgcatttct ttcaataatt ccacactgat tttttcaaca
       61 ggtgtttctt ttttgaaaaa agattttttt acattgatct tatctgacaa gatcgaactt
      121 aatttactag caaaaatcct ataaaattca tctaaatagc gataataaat ccgattcagc
      181 tgatcattaa gatcgaagta tacaaaatta ttctgtagtt tataaaaaaa cggcgaatga
      241 agatgtgttt tcatatgacc aaaatataat aattctgaaa tttctaaagg tgtcaattct
      301 ttcagcatcg ttaaatcagt aaagtcgatc cacttcaatt catcgaatcc acgcctgtta
      361 cgagaagagg aaaagaaacg ctgaatattt tctgtaccac ggattacttt cagccctgta
      421 tgggattcat attctccagc agaagcagat ggatcaagca agagcaagtt ttgaggaaaa
      481 tgcacgatac tgtttgcaaa gtcagtagca gtcagccctt ttgttaaaac agcattactt
      541 gtggtatcaa tatgtacata aactgactca gtcatcatgt tcctcctctt atttcctctt
      601 aatctctttt attttacaac atttgcatta aaaaagcttg tttcttttat tttaaaattc
      661 cattacaaaa tcacgttttc attgaaaaca ctctcatttt caattaattt ttgtgatttt
      721 tgtatttttt tatttaaagg aggttttatg aattgttttc ttatggaatc tacaaagtcg
      781 aatacgagac tcatgacctt tttatgacgt actggtatac ttgaagaaaa aggagagcgc
      841 gatggagata aaaaaagttt acttttcaca gaaaatgtcg ctgtattcgc ctatccagca
      901 agcaaaacaa ccccttccat tttttcaaag tcatcaagaa agtagagacg ctttttggcg
      961 ttctgcaaaa aagcaatgtg cttttaaagc agtatcgaaa aaatataccg tctattttac
     1021 tattgctaaa agtagaggag atgttatttt taataatctg ttgattgaag gtgaaacttt
     1081 tgaatggaaa aaattttttc ggtatatgga agcttgcgct cgttttttta ttaaagaaaa
     1141 ctgttgtttt ttatttcaat ctcctctatc agaagaatgg ttgcgtattt ttcataaaaa
     1201 cggctatcaa ggaaccacac aattaaataa aaagctggtc tatcatacag cacttgtttt
     1261 aggtggagga ggagcgcacg gtgcttatca gattggtgtg tggcaggcgc tgaaggaaca
     1321 tgatatcaac tttgagatta tcacaggtac ctcggtaggt gcgttgaacg gcgcattgat
     1381 tttacaagga gatatgaaaa aagctgtgaa tttatggaaa aagctgtcga caagacaagt
     1441 gcttgcgtta ccagaaatgg ctgcagttga agaccttcgt gaacgcttct atcgagaaag
     1501 acgccaaatg acgaaaacag cactaattga aggcggggtt tcttctgctc cattggaaaa
     1561 gatggtcaaa gacaatttgg atttgtcctt actcaaacat aatcccaaaa tacgtttata
     1621 tacggtgtct actaaactgc cagaactagc ggaagtagtg actgatatcc aacagacaaa
     1681 aacagaagaa ataccagatt ggatactcgc ctctgcttca ttttatcccg ctatggctta
     1741 tcgaaagatc ggaaaaaata aatatgcaga cggtgggtat cgcaacaaaa tacctattga
     1801 tattgcgatt aacaaaggag caacagaggc atttgtcgta gatgtgcaag ggccaggccc
     1861 agcgaaaaaa attcgtatgc cagacatttt tatccattgg aaatgtcaaa cattgtggac
     1921 actaggcagc tttctgcttt ttgatagcca aaggaaccaa ttaaatttac aattaggcta
     1981 tctggaaatg aaaaaacgat taggctatta ctatgggaac tggtatacat ttcattctgt
     2041 taaacctgca agaacatact ggcgaggatt tttgtcttac ttaacaaaag aaattcagtt
     2101 agaattatct tttttcaaaa gcataaaatt ttggcaaaaa ctgcgtaatt tatataagaa
     2161 cagggtcgta ccagagacat gcggattggc gatgctagaa cttttagcaa aaaaacattt
     2221 tttactgccc aatgaaatct atcaagtaga catgatgatt caaatgatct gccaacaaga
     2281 tataaaaagt atgccggatg atttattggc acaaattggt caattaagta ctgaagaatg
     2341 gcgaaggtat cgaaaatacc agcaaaaaat acaaaatgag cgcacgaaaa ctgtttattt
     2401 tgattatctg ctacaaggaa aacgcaagga tcgtttgcag catgaattgc ttaagcaacc
     2461 agttgacaca ttactgatcc tatatttata ttatctcaag gaggaacaac catggcacaa
     2521 gaattttcat atgaaatcgt agaagaaatc gctatattgt cagaaaacaa caaggggtgg
     2581 cgtaaagaac tgaacctagt cagctggaat ggtcgcccgc caaaattcga tcttcgagat
     2641 tgggcgccag accatgaaaa aatggggaaa ggcctgacat tgacgaacga agagtttgaa
     2701 caactgcaaa aagcaataga aaatatgtaa tgaggaagga ccctgtcgaa tgaaaagtat
     2761 gactggtttt ggcaaagcca tccgcgaaac aaaagcgtat cagttagata ttgaaataaa
     2821 aagtgtcaac cagcgttttc tagatgttca aatacgaagt cctaaaatcc tgaactttgt
     2881 tgaaaacgat tttcgtcagc ttattaaaca gcagttaagc cgcggtcgaa tcgaagtatt
     2941 tgtgaatcta gcttatattg gcaaaaacga aaaacagatc gtcatcaact gggaattgat
     3001 tgattcactt atcactcaat tgacagacgg aattcaacaa cgatacggta gtcaaaatca
     3061 agtgtcaatt agtcggatgg cggaacgctt cgcgctggac gaaaacttcg taacggtaga
     3121 agaaaaaccg gaagagagca tggaagaatt ggcacagtta gctctagata cattgaaaag
     3181 cgcacttgat tctattgagg cgagtcgaga aaaagaaggt caagctttag ctttagtgtt
     3241 gaaaaagaat actgaagaat tcaaagaagt actaaaaaaa ctcgatagtt ttgtagaaat
     3301 ctatgaaaag gaatttcaaa taaggtatca aaaaaagctg gaggaatttc ttggagcgaa
     3361 agtcgatgag caacggttgt tgacggagct agccatcttg ttggaacgcg gagatattca
     3421 cgaggaactg gatcgtctgg cgatccatgt acaaaaacta gatgaactta tcgaagcaca
     3481 atgtcctgta ggtcgggaat tagatttcct gatccaagaa atgaatcggg aaatcaacac
     3541 gatcggttcc aaatcaaatg caatcgaaat taaaaatcaa gtcgtccagt ctaagacgat
     3601 cttagaaaaa atcagagaac aagtacaaaa tattgaatga cgaaagaggt agagataatg
     3661 aaatattatg acgtaacgtt ccacgaatta agtggaaaaa ctgtggttaa acgggatatc
     3721 ccttctgaaa aaaacggctt tgacgtatgg aaagatgcct gtgcagacta taatgagaac
     3781 gaactgttta ttttgatcaa cgacggtgct tacgtgacga tgaatcggaa atttattgtt
     3841 cgaattgaca cagaagaggt agaagatccg acagaaaaag cacgttctcg aaaagatgag
     3901 atcatgggtg tagtgaatac tttgtccaat atgggctttt agcaaggatg ctgaacaaag
     3961 aaatacaata aataatcaga attggatttc ttcgcttagc ttctttgctc taggaataca
     4021 tttaaaggtt gtgacaaaat tcgtgtcaca atcttttttt attttgaata agtgatgttc
     4081 aaaaaccatt ttctcggtat t
//
LOCUS       DAZKLD010000183         4042 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.186, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000183 DAZKLD010000000
VERSION     DAZKLD010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4042)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4042)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4042
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.186"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            237..557
                     /locus_tag="ACG8KR_002728"
     CDS             237..557
                     /locus_tag="ACG8KR_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HFQ0202037.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGLLLISIIGIKI
                     TSGH"
     gene            718..1623
                     /locus_tag="ACG8KR_002729"
     CDS             718..1623
                     /locus_tag="ACG8KR_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0202038.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSEGTIRIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVKQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDQAARTILTDAGYPLEDPDKIKLLKKNEELLGTVIQLLSAENVHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            1620..2393
                     /locus_tag="ACG8KR_002730"
     CDS             1620..2393
                     /locus_tag="ACG8KR_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0202039.1"
                     /translation="MTLLIKQELFKLIKKKSTAVLSVLLVALLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMESQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSIYLYLLAIIVTVLMKLILFPSISFTEKISTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIALFLALGYLVFKRKNV"
     gene            complement(2570..3994)
                     /locus_tag="ACG8KR_002731"
     CDS             complement(2570..3994)
                     /locus_tag="ACG8KR_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="basic amino acid/polyamine antiporter"
                     /protein_id="HFQ0202040.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNVASIVMSTIFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFINNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGFLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
BASE COUNT         1280 a          819 c          747 g         1196 t
ORIGIN      
        1 ctatgaaatt gcaaagcatt catcttcttt tctgctaacg atatatccgt caatgcttgt
       61 tacactttac taaaaagtat gagattgccg atatctggat aggtgtgcta tactacagaa
      121 aaagatatac gatctgccac cgggtagtaa aaaagaggat gatcattcca ttaggtcttt
      181 gaagggatga ttctcctctt tttgtgtatt ttttatattg aaggaggaac gtatgtatgg
      241 catggatgga attaatcata gcaggaattt tggaagtatt ctggtcaacg atgatgaaat
      301 ggagtgatgg tttttctaaa atcaattatt ctttttacac agtgattgga atgattgcta
      361 gtttttactt tttgtcaaag gcaatcaaaa gtttgcctat gagtctctct tatcctattt
      421 ggacagggat tggcgcagtt ggttcggtca ttattggtgt ggtcttgttt catgataagc
      481 tgaatctttc cacttggttc tttgtcgggt tgctgctcat cagcatcatt ggcatcaaaa
      541 tcactagcgg tcactagata cttagacaag cagacaagga ctgatcagaa aaagcggggc
      601 tttttatttg atattcctct ttctatgaaa aaacaagaaa ataagactaa tgacctagcg
      661 acttctctac tcatcaggtg tatgcttgta ctatctatag aaaaggagtg aatatttttg
      721 aaaagtcttt cggtccaaga attatccaag aactttggtc acaaacaagc cctcagcgat
      781 gtcagtttta gcttatctcc tggcaaaatc gttggattag taggtcctaa tggcgctgga
      841 aaaacaacga tcatgaaagc tattttagga ctgatccatt actcagaagg aacgatccgt
      901 attggtgaaa atgagatttc tccctctagt cataaaggat tagaacaagt aggagcattg
      961 atcgaatatc cgggtatcta tccattctta acaggttatg atcatctgaa gttattctct
     1021 gaaacaaacg atgtatctgc tatcgacact atcgtgaaac agctcaagat ggaaaaatac
     1081 attcacaaaa aagcgaaatc ctactcgctg ggaatgaaac aaaaattggg aattgctctt
     1141 gccttactga accatccgtc tatcgttatt ttagatgagc cgatgaacgg tttggatcca
     1201 caagcaacaa gagatgtgcg aaaaatcatt accgacttgg ctactcaggg cacctctttc
     1261 ttgatctcca gtcacttact cagcgagctt gaaaaaatgg tcgatgagct gatcctgatc
     1321 aatcaaggaa aaatcatcaa acaatgtaca atggctgaat tgaaccaaac agcgcaagat
     1381 ttcatcgtta tctctacgac aaatgatcaa gctgctcgaa cgatcttgac agacgctgga
     1441 tatcccttgg aagatcctga taaaatcaag cttctaaaaa aaaacgaaga gttattagga
     1501 actgttatcc agttattgtc tgcagaaaat gtccatgtga cagatgtcca gcatctgacg
     1561 aatgatttgg aatcttccct attggaaatt ctggattcag taaaggggga agaagaaaaa
     1621 tgacgttgct tataaaacaa gaattattta aattgatcaa gaaaaagtca actgctgttt
     1681 tatctgttct attagttgct ttgctcatag gaacagcact tctagccaaa aaatacacga
     1741 cgatcattga tccagttgaa atgactgctc agcttttcag tgccacatct tggattgtct
     1801 ttatcatgat tgctgctgca agtacgatca tttcaatgga atcacaatat ggtacattga
     1861 aaaatctcct ttatcgaaag tattctcgag gagaaatatt agtaagtaaa tggatcacat
     1921 tagttattta ttctatttat ttataccttt tagctattat tgtcaccgtg ctgatgaaac
     1981 tgatcctttt cccttcgatt tcctttacgg agaagatttc tactggtcag acactgatcc
     2041 aaagcttatt cacttatact ttgggtagtt atatcggttt atggctaatt ttaagtttag
     2101 tgttgatgat tgcttgtttt atcaatagtt ccggtgcctc catctcagca gggatcgtct
     2161 tttattttgc ttcatcaatc atatctggta tcctcatcgc actcattcaa aaatgggagt
     2221 ggatcaaatg gaatccaatc agtatgctga atctgcaaaa ccaaattggc aacgaagaga
     2281 ttatgaagca attgactcat ctttctacta atcaaatgct gtttggcaat ttagcttaca
     2341 ttgctttgtt cttagcattg ggatacctcg tattcaagcg aaaaaatgta tgatggttca
     2401 ttaacaaaga agttgtgaca gaagtggcca gtttcaagaa atttcggctt atttttagaa
     2461 gaagctactt cttgaacacc atttattcaa aaaaagaagc gatgacacga atgttgtcac
     2521 cgcttctttt ttgaataaac cacaggcttc acagcctctc tttatacttc tatacgtgaa
     2581 gcatcccagt ggcaaataaa accattgcga ttactccgac catcgtcaca ccgataagta
     2641 aaatcttatc taagtggata tgttgtacag gttcgttttg ttctttacgt gctttgaggt
     2701 aaaaataaat acctggtaag aaaagaacaa gaacaagcat cacttcatta aaggctacaa
     2761 ctagcatggc ccatacttga aaagcacttg ctaaaaaccc tactaaaaat tggacccact
     2821 ctttccgctg ataagaaagc ttcatttggt acagccctaa gaaaagccac gtgaagaaaa
     2881 tcgctgctgt accaagtgta taagcaaagt tatatgcact gtcagtgaat aataatgaaa
     2941 gtaaaaagac attcgtacat aacgtcgtca atacgatagc ataagttggt gccccatttt
     3001 tgttgagttt tccccatgct ttaggcaaaa gtccgtcctc agccatattc cccattgttt
     3061 caatcggcaa gattgtccat gagagccaaa ccccaactaa agaaatgatc aaaccgccgt
     3121 tgatcacgac cgccccccat ttaccaacca tcatttcaaa tacatatccc atagcaggtt
     3181 gcgctttgat cgtttgcagc tgattcatcg tcattgtgcc atatggaagc atggaaacca
     3241 agacgtaaat aatgatcaaa ccgaatattc ctagaatcgt tgctttccca acatccgatt
     3301 gtttttttgc tcgtgaacct acgacagaag ctccttcaat accaacgaat acccagagca
     3361 tcaccatgat gcaactcaat acttgactag aaaacgggat cggctgccca ttcgcattca
     3421 atatgatatc gctagaaaag ttattaatga attgttggat cgaaaaggtg ctgatagcta
     3481 ctacaataaa taaaaataat ggcactaatt tacaaaatgt gacgatagca ttgacaaagc
     3541 tagcattttc aactccacga ttcactagcc atgcatatcc ccataagaag atcgtactca
     3601 tcacgatcga tgccacattt tgaccacctt cgaatacggg gaagaaataa cctaatgcac
     3661 tcatcaacaa tgttgcgaac gccacgtttc ctaaccagca tgtgagccag taaccccaac
     3721 cgctgataaa accagcgaaa cggccaaaac cttcttctgc gtaactaaag atcccgcctt
     3781 ttaaatctgg acgtttttta ttgatattat tgatcgataa ggagagcatc aacattgctg
     3841 ttcctacgag tacccagctg aagatagctg ggccaccagc cgatccggag atgtccgtca
     3901 taataccaaa tacgccacct ccaatcgctg aaccaatcac taaggcagca agtggaatca
     3961 aaccaattcc ttttttctgt tgctctgtat ccatgtaagc atcctccaaa gtaaaaccgc
     4021 ttttatcaaa aagtataaca aa
//
LOCUS       DAZKLD010000184         3994 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.187, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000184 DAZKLD010000000
VERSION     DAZKLD010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3994
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.187"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            21..1961
                     /locus_tag="ACG8KR_002732"
     CDS             21..1961
                     /locus_tag="ACG8KR_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-specific PTS transporter subunit EIIC"
                     /protein_id="HFQ0202041.1"
                     /translation="MKKIIAITSCPVGIAHTYMAAENLEKAGKAVGAEVKVETHGSIG
                     IENELTARDIEEAAGVIIAADTKIDKSRFGGKPLITVGVQEGIHHADELVRDILAGKA
                     PVYKSTEAIISSENKSESENLGKKIYKSLMNGVSYMVPFVVTGGLLIAISLTLGGTAT
                     PEGIKIPEGTIWATMNSIGSIAMGLMVPILSAFIAQSIADRPGLVPGFVGGMLAANGA
                     LYGSDANAGFLGGIITGFLAGFIALGIKKVRVPKALQSIMPIIVIPILGSLAVGFVFI
                     YVIGEPVALLFSSLTHFLAGMQGGSEIVLAMILGAMIAFDMGGPFNKVAFLFGSGLIA
                     EGNYSIMGPIAVAICIPPLALGLASLILKTKFTKTEREAGKASLAMGLFGITEGAIPF
                     ASTDPLRVIPSIVIGAMTGSVIAMLSGVTDHVAHGGPIVAVLGAVDHVAMFFVAVIAG
                     IAVTVLMLRILKPTLQQTAVAAANGVTVADLSETETSDVYTKTETFDSITDIMSDEAI
                     VLGETATTKDGVIEDLVQRLEKTAAITDKTRFKEVIYDREHESTTGIGMGIAIPHGKS
                     AYVLKPTVAFARSQSGVDWHSLDGKPAHMIFMIAVPENSQGDMHLKILQRLSRKLMDD
                     DFRQALMEAPDKTTVHQLLSEM"
     gene            2048..3970
                     /locus_tag="ACG8KR_002733"
     CDS             2048..3970
                     /locus_tag="ACG8KR_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFQ0202042.1"
                     /translation="MNQRQQSLIYQLSENNNYITTKKLAEHFHVSERTIHSDLNTIET
                     WLRDKHLPLMFERKKGTGILLNGLPHEREQLKRALNEETQTEMDKKQLRQLLIFHLLV
                     AKDGFSLEELSEKLYVGKRTIRKELTELQSFFEYHHLQLVSKTKLGTFLKGDEQEKRQ
                     LLVKTLRNMQKEDPQSPSLKEFFAKDTLKVIQHTLQDVFYENHLEQPNGLASVEIHIY
                     FMLERMKQYQKVKLSKDENEVVEHTQAQKLSSQILAKLATIYPIEFSPDEINYLALRI
                     ANLFTNSQAATRFQKESSALADHLIEQVEQFLGYSLKEDQLLKQNLRSHLSSTYFRLH
                     YGLQISNPLTKNVFSTYTQLFLVLQLILEDYFEKENFYVPQEETAYLTIHFQAAIERH
                     KHQKSRRYQTVLVSEYSKAMATFLEARLNRELPELTIMDLVEYKPELKRQDFPSVDFI
                     LATLPFKHISIPVIEISPMITETDLAYLTKYMLEHVPIKKKKTFDLASFTHPFLIFPQ
                     LERTDPADILNFMGNVLVEHHYVEPEFIDSVLERDSHSSTRVAPFVTIPHGNPLYVKH
                     SMISIATMKEPVLWHGEQIRIVLLLAIKKNDLKHAEFKKIFSVIHYLEKQPEQMAQLM
                     QSNHPLEILTLLSSYE"
BASE COUNT         1344 a          688 c          842 g         1120 t
ORIGIN      
        1 tgtagaaagg cggtaggaaa atgaaaaaaa ttattgcaat cacatcctgt ccagttggta
       61 ttgcccatac atacatggca gcagaaaact tagagaaagc ggggaaagca gttggcgccg
      121 aggtaaaagt cgaaacacat ggttccatcg gtattgaaaa tgaattgact gcaagagata
      181 tcgaagaagc agctggtgta atcatcgcag cagatactaa aatagataaa tcacgattcg
      241 gcggtaaacc tttgataacc gtaggtgtgc aagaaggaat ccaccatgca gatgagttgg
      301 tgagagatat tttagcagga aaagcgccag tctataaaag tacggaggca attatttctt
      361 ccgaaaataa aagtgaatca gaaaatttag gtaaaaaaat ctataagagt ttgatgaatg
      421 gcgtttcgta catggtacct ttcgtagtta caggagggtt attgatcgcg atttctttaa
      481 cattaggagg aacagctaca cctgaaggga tcaagatacc agaaggaacg atttgggcaa
      541 cgatgaatag tatcggaagt atcgctatgg ggttgatggt tccaattcta tctgcattta
      601 ttgcgcaaag tattgcagat cgtccaggac ttgttcctgg atttgttggt gggatgttag
      661 cggcaaacgg agcattatat ggtagtgatg cgaatgcggg attcttaggc gggatcatca
      721 ctggattttt agcaggattc attgctttag gaatcaagaa agtgcgagta ccgaaagctt
      781 tacaatcgat catgccaatt attgttatcc caattttagg atcattggca gttggttttg
      841 tgtttatcta tgttattgga gaaccagtag ctcttctatt tagttcattg actcactttt
      901 tagcagggat gcaaggagga agtgaaattg ttctagctat gatcttagga gcgatgattg
      961 cttttgatat gggggggccg ttcaataaag tagccttttt gttcggatct ggcttgattg
     1021 cagaaggcaa ttattccatt atggggccaa ttgctgtagc tatctgtatc cctccattag
     1081 cattggggct ggcatcattg attttaaaaa ccaaatttac gaaaacagaa cgagaagctg
     1141 gtaaggcgtc cttagcaatg ggactgtttg ggattacaga aggcgctatt ccgtttgctt
     1201 caacagatcc actacgggtg attccaagca ttgtaatcgg ggcaatgaca ggaagtgtga
     1261 tcgcgatgct ttcaggagtg acagatcatg tagctcatgg cggtccgatc gtagcggttt
     1321 taggagcagt ggatcacgta gccatgtttt ttgtagcagt gattgctggt attgctgtaa
     1381 ccgtgctaat gttaagaatc cttaaaccga cattacaaca aactgcggtt gctgcagcaa
     1441 atggtgtgac agtagcagat ctatcagaga ctgaaacaag tgatgtttat acgaaaactg
     1501 aaacttttga ttctatcacg gatattatgt cagatgaagc aatcgtttta ggtgaaacag
     1561 caacaacaaa agacggagta atcgaagatt tagtccaacg tttagaaaaa acagcggcga
     1621 tcacagataa aaccagattt aaagaagtca tctatgatag agaacatgaa agtacaacgg
     1681 gaatcggtat ggggattgct attccacatg gaaaatcagc ttatgttctt aaaccaacag
     1741 tggcatttgc cagaagtcaa tctggggtag attggcattc tttagatgga aaacctgcac
     1801 atatgatctt tatgattgct gtacctgaaa acagccaagg tgatatgcac ttgaaaatac
     1861 tgcaacgtct atcacgtaaa ttgatggatg atgacttccg tcaagcactg atggaagcgc
     1921 cagataaaac aacagttcac caattattaa gcgaaatgta acataagatg aaaaatcgac
     1981 cgtgatgaga cataatagat gtgttatcat gatcgatttt ttatttagaa gggagtttct
     2041 gcacattatg aatcagcgtc agcaatcttt gatctatcaa ttaagtgaga acaataatta
     2101 tataacaaca aaaaagctag cagagcattt ccatgtgtca gaaagaacga ttcatagcga
     2161 tttgaatacc attgaaactt ggttgagaga caaacatctg cctttaatgt ttgaaagaaa
     2221 aaaaggtaca ggtatcttgt tgaatggtct gcctcacgaa agggaacaac tgaaacgagc
     2281 gcttaatgaa gagactcaaa cagaaatgga taaaaaacag cttcgtcagt tattgatttt
     2341 ccatctgcta gtagcaaaag acggatttag tcttgaagaa ctttcagaaa aactatatgt
     2401 tggaaaacgt acgattcgta aagaattgac agagttgcaa agcttttttg agtatcacca
     2461 tttacagcta gtatccaaaa caaaactagg aacttttctt aaaggcgatg aacaagaaaa
     2521 aagacagttg cttgttaaga cattacgaaa tatgcagaaa gaagatccac aaagtccgtc
     2581 gttgaaagaa ttcttcgcta aagatacatt aaaagttatc caacatacat tacaagatgt
     2641 tttttatgaa aaccatttag aacagccgaa tggactggct agtgtggaga ttcacatcta
     2701 ctttatgctc gagcgaatga aacagtacca gaaagtgaaa ttaagtaaag acgaaaacga
     2761 agtcgtggaa cacacccaag cacaaaagtt aagtagtcaa atattagcaa aactagccac
     2821 catctatcca attgaatttt ctcctgatga aatcaattat ctggctttgc gtatcgccaa
     2881 tctttttaca aattcacaag cagccacacg ttttcaaaag gaatctagcg cacttgctga
     2941 tcatttgatc gaacaagtgg aacagtttct aggctattca ttaaaagaag atcagctact
     3001 aaaacaaaat ttaaggtctc atttatcttc aacttatttt cgactccatt atggtttgca
     3061 gatttctaat ccattgacga aaaatgtgtt tagtacgtac acacaacttt tcttagtttt
     3121 acagttaatt ttagaagatt attttgaaaa ggaaaatttt tatgttccac aagaggagac
     3181 agcttattta acgatccatt tccaagcagc gatcgagcga cacaaacatc agaaaagtag
     3241 aagatatcaa acggttttag ttagtgaata ttcaaaagct atggctactt ttctcgaagc
     3301 acggctgaac agagaacttc cagaattaac gattatggat ctagttgaat ataagccaga
     3361 attaaagaga caagattttc cctctgttga ttttatcttg gctacgttac catttaaaca
     3421 tataagcatt ccagtaatcg agatctcacc aatgatcaca gaaactgatc tagcctattt
     3481 gacaaaatat atgctggagc atgtgcctat caagaagaaa aaaacatttg atctagcaag
     3541 ctttactcat ccatttctga tctttcctca acttgaacgg acagacccag ccgatatttt
     3601 aaactttatg ggaaacgtct tagtggaaca tcattatgtg gagccggaat tcatcgattc
     3661 tgtcttggaa agagacagtc actccagtac tagagtagca ccatttgtaa caatccctca
     3721 cggtaatccg ctgtatgtga agcattcgat gatttctatt gctacaatga aagaaccagt
     3781 cctatggcac ggggaacaga tacgtatcgt actgctatta gcaataaaga aaaatgattt
     3841 gaaacatgca gagttcaaaa aaatattttc tgttattcac tatctagaaa agcagccaga
     3901 acaaatggct cagttgatgc aatccaatca tccgctagaa atcttaacgt tgttatctag
     3961 ttatgaataa gtagcgatta aaaaactgcc ttta
//
LOCUS       DAZKLD010000185         3992 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.188, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000185 DAZKLD010000000
VERSION     DAZKLD010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3992)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3992)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3992
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.188"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            54..1355
                     /locus_tag="ACG8KR_002734"
     CDS             54..1355
                     /locus_tag="ACG8KR_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFQ0202043.1"
                     /translation="MEKLMKWVEEKLVPPMAKIGTQRHLLAIRNGVVSTLSLILIGTF
                     FMVFINLPFPGWNEFIAPYSATIVLPFRMTMGLMAIYATFVMGSDLAKSYGLDSVTGG
                     ILSLGTFFMLQVPVNVLTPEEAPLGWVLPMSSLGASGMFAGILSMIFAVEVYRFFKNR
                     NITIKMPEQVPPAVARSFEALIPGAVILTITWLIRSVAGFDVNAALLSLFEPLTNILG
                     NNLLGVLLPMFLIHLLWSFGIHGMSIVGAVVRPMWLIMLDENAKALADGTAATQLPYI
                     APEQFYQWTVTIGGAGATIVVSVLFIFFCKSKFLKEVGRFSIIPGIFNINEPIIFGAP
                     MMLNPYMFIPFNLVPLVLTIISYFAVKLEMVNRFTVLPTWTLPAPIGGYLSAGNDWRV
                     VILIMINTLIAFIIYYPFVKAYDKKMLSDEQSRGENEVQTV"
     gene            1382..1642
                     /locus_tag="ACG8KR_002735"
     CDS             1382..1642
                     /locus_tag="ACG8KR_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFQ0202044.1"
                     /translation="MYWLILFFVFIFLLTASHLILNMLATYHIQINRWIWALASFLIV
                     ILPKIIVTHLNVLFSWGTYVLCGIFAINFMIEQHRWFVTSKL"
     gene            complement(2641..3876)
                     /locus_tag="ACG8KR_002736"
     CDS             complement(2641..3876)
                     /locus_tag="ACG8KR_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFQ0202045.1"
                     /translation="MKTILFAPAVFNLAETTRMIEVAKAMKDRYYCVFFGFSKTFAPL
                     ITESGFEYHLLAPILTKKQEEQIIKFDQLRTLKNPFTKEMVASRITSEQSLIAKFEPR
                     LIVTGSNVTIFLSARNKKIPLVYVKPYALSEPFFQSEHALMPHELRFFGPLSNPLWHW
                     TKKRIVNTRWIPNAFKHAAQKEMLPLPKTSLQMMDADLNLITTPKLFLEEEQLPENYR
                     IVGPIFAKLSTSLPEEVLTFIQRKQQERKRIIYFAMGSSGNPRMLRKILFFLQKQPDL
                     AVIAPIDYLLDDIQSDSEDLLITEYLSSHLLHEFIDFSFIHGGEGTVQTACESGKPFI
                     GVGMQFEQSCNIDYCVKYGNAIALTKPVTERKLQIALREVSSPKIRQQARTLQAVFPI
                     NGPQQAIQEIETFLSNHPI"
BASE COUNT         1124 a          648 c          847 g         1373 t
ORIGIN      
        1 ttgcgttgtc atgcaatgaa tggttcatat tttattgaaa aggagtggtt ttagtggaaa
       61 agctgatgaa atgggtcgaa gagaaacttg tcccaccaat ggcaaaaatt ggtacacagc
      121 gtcatttatt agcaattcgt aatggggttg tttcaacatt gtcattgatt ttgattggga
      181 cattttttat ggtctttatc aatttaccgt ttcctggatg gaatgaattt attgcccctt
      241 attcggcaac gattgtttta ccctttagga tgacgatggg attgatggct atttatgcaa
      301 cttttgttat gggttcggat ctagctaaat catatggatt agatagtgtg acaggtggta
      361 tattatcttt aggaacgttc tttatgttac aagttcctgt aaacgtttta actcctgaag
      421 aggcacctct tggctgggtc ttacctatga gttctttggg agcatctggg atgtttgcgg
      481 ggatcttatc tatgattttt gcagtggaag tatatcgttt ctttaaaaat cgaaatatta
      541 ctatcaaaat gccagaacaa gtgccacctg ctgttgcccg tagttttgag gcattgattc
      601 caggtgcagt cattttaaca attacttggc tgattcgaag cgtcgcgggc tttgatgtaa
      661 acgctgcctt attatcgtta ttcgagcctt taaccaatat tttagggaat aatttgcttg
      721 gtgttttact accaatgttt ttgatccatt tgttgtggtc atttggtatc catggcatga
      781 gtattgtagg ggctgtcgtt cgtccaatgt ggctgatcat gttggatgag aatgcaaagg
      841 cattggcaga tggtacagct gccacccaat taccctatat cgctcctgaa cagttttatc
      901 aatggacggt aacaattggt ggtgcaggag caacgatcgt ggtatctgtt ttgtttatat
      961 tcttctgtaa atcaaaattc ttgaaagagg ttggtcgttt ttctattatt cctggtatct
     1021 tcaatatcaa tgagcccata atttttggag ctcctatgat gttgaatcct tacatgttca
     1081 tcccgttcaa cttagttcct ttagttttga ctattatcag ttattttgca gtcaaattag
     1141 aaatggtcaa tagatttact gttttgccaa catggacatt accagctcca atcggcggtt
     1201 atctttcagc aggaaatgac tggcgtgttg ttatcttgat catgatcaac acattgatag
     1261 catttattat ctattatcca tttgtaaaag cttatgataa aaagatgctg tcagatgaac
     1321 aaagtcgtgg agaaaacgaa gtccagactg tctaagtttg gtttttagaa ggtgggaaac
     1381 aatgtattgg ctgattttat tttttgtttt tatcttttta ttaacggcta gtcaccttat
     1441 attgaatatg ctagctacat atcatattca aatcaatcga tggatttggg cactggcatc
     1501 atttttgatc gtgattcttc caaagatcat tgtaacgcat ttgaatgttt tgttttcatg
     1561 ggggacatat gtactttgtg gaatttttgc tatcaatttt atgatcgagc agcatagatg
     1621 gtttgtcaca tcgaagcttt aaatgttcta aaagtggttg taacagaagt ggttagcttc
     1681 aagaaataag aagaaattta cgaaaattgc ttcttaaatt tttgtgaaat gttggcttat
     1741 ttttcgtttg gttgaactta ttaccgcaga tgcaagcttt tgaccaccgt ttattcggaa
     1801 tatgggactg tgacaaggct tttgtcacag ccctttttta cataaagatt attattgttt
     1861 taactgtttt ggcagaaaga aggaggaaaa aatggaaatt ttatcagcaa tggatttaga
     1921 attagtagat ctactaccaa agtgatcaag tcaaatatag atttgggagc ttacaaccat
     1981 atagtagggg ctgcagtatg gtgtatgaat ggtaatatgt atgatgtgag tgtttattca
     2041 aatcatgaaa cgtgtataga aataattgtg ctgggaatag caatcagtca tggtgaacgt
     2101 gacttttagt gtatcgttgc tgacgacata gaacattctg acatgatcta ctcaatatgt
     2161 ggggattgct ggcagtttat ataggagtat gcatcggatt gcgagatcat tgtctcaaca
     2221 gaagagggag gttgattatc aaagataaag tggtaaatcc tacatttgat ttagtaattg
     2281 ttgaggggac tagcggcaca aatctctaca aagcgatgct tctagatcta tatgacacat
     2341 tttaagaaaa tgtatttgcc taattattat ggtctttctt ttgaagaaac gttgagatga
     2401 tccaaccaga gaaaggtgtc aaagaatttg actaagaata aatggcagac gctacctatg
     2461 ctcatttgga tagcgtcaga tcttttattt taaggatgat tttgtgtttt ttagacaccg
     2521 taatttaagt aactctaact tttcaatcca ctatttactt tttttgtatt ttcactgtct
     2581 gggtctcgaa tagcgaaatt actgatgagt ctctattaaa aaagcttttt ctacttgaaa
     2641 ctatattggg tggttgctca ggaaggtttc gatttcttga attgcttgtt gtggaccatt
     2701 tattggaaaa acggcttgca gcgtgcgtgc ctgctgacga atcttgggtg aagaaacttc
     2761 gcgcagagca atctgcagtt ttctttcagt cactggtttt gtgagggcga ttgcgtttcc
     2821 gtattttaca caatagtcaa tattacagct ttgctcaaat tgcatcccca caccgataaa
     2881 cggtttgccg gattcacagg cagtttggac ggtcccttcc ccgccgtgga taaaagaaaa
     2941 atcaatgaat tcatgcaaaa gatgactaga cagatattct gttattaaaa gatcttctga
     3001 gtcagattga atgtcatcaa gcagatagtc aataggtgca ataaccgcta gatccggttg
     3061 tttttgtaag aagaacaaaa ttttgcgtaa catacgcgga ttgcctgaac tacccattgc
     3121 gaaatagata atacgttttc tttcttgttg ctttcgctgt ataaaagtta gcacttcttc
     3181 aggcaaggaa gtactgagct tagcaaaaat tggaccaacg attcgataat tttccggcag
     3241 ctgttcttct tccaaaaata gtttaggcgt tgtgatgaga tttagatcag catccatcat
     3301 ttgtaaactt gtttttggta aaggaagcat ttctttttgt gcagcgtgtt tgaaggcatt
     3361 ggggatccat cgggtattta ctatacgctt ttttgtccag tgccaaaggg gattgcttaa
     3421 agggccgaag aaacgtaact cgtggggcat cagcgcatgc tctgattgaa aaaaaggttc
     3481 actcaaagca tagggtttaa catatactaa aggaatcttt ttattgcggg ccgataaaaa
     3541 aattgtcaca ttcgaacctg tcacaatcag tcgaggctca aacttagcaa tcagactctg
     3601 ttcactagtg atacgagagg caaccatttc tttggtaaaa gggtttttaa gtgtacgcaa
     3661 ctggtcaaat tttatgattt gttcttcttg ttttttcgta aggatcggcg ctagtaaatg
     3721 atactcaaag ccactttctg tgatcaaagg agcaaaagtt ttagaaaagc caaagaatac
     3781 acagtaataa cggtctttca tagctttggc aacctcaatc attcgtgttg tctcagctaa
     3841 attgaatact gcaggagcaa aaagaattgt cttcatgaaa tcctccttat tggaatgagt
     3901 ctatcccttc gtttcttact agtatactaa aaaacacagc gttcgagtag cctatgtata
     3961 ttttgaatat taaactgtct ctcgctgttt tc
//
LOCUS       DAZKLD010000186         3818 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.189, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000186 DAZKLD010000000
VERSION     DAZKLD010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3818)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3818)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3818
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.189"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            748..1716
                     /locus_tag="ACG8KR_002737"
     CDS             748..1716
                     /locus_tag="ACG8KR_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFQ0202046.1"
                     /translation="MSNEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMRDVGDKKVQ
                     FTFEQLKDLSNYKATANVRFIDDLETTYDKLMDLRFGRRSADGLQRERFVLFNQFKID
                     GKSDIPFAEIQVHEKALPLLNNLEEWVRYSLQQFNELESSYSKTMFRLLKQYRTKGFA
                     YFSKEDFHELLDIPKSYNQGTIDQRVFNPIRQELTAIFKGLTIKKKYGKGRGKPVIGY
                     QFTFKPEIKKSDDFNKHAPTKEEQNKALELANQQVQSEKAPTSLQEKIKEQKRKLELL
                     QQLEKLEREQNTQNKENNAQKSEINSDIPSELKKDLLDNLQNLFKD"
     gene            1733..2257
                     /locus_tag="ACG8KR_002738"
     CDS             1733..2257
                     /locus_tag="ACG8KR_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HFQ0202047.1"
                     /translation="MTEKTIKELAEELGVSKTAINKKVSDENRKLWFSKIGNKFVINE
                     TGQKAIKSMFFNENENSNQQLVGEKTKTENHKNANQVPELEVLSLLEQQLNQKDMQLQ
                     EKDEQIKQMQKLLDHQQQLTLQANQQIEKLQEQLQLTYTEESSENKSTTLSEETESIE
                     KQSKAEKKWWQFWQ"
     gene            2633..2914
                     /locus_tag="ACG8KR_002739"
     CDS             2633..2914
                     /locus_tag="ACG8KR_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000353845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0202048.1"
                     /translation="MDILVRNINPKIIARIDKRCQELTTKTGKKWSRNKYLKVLIESD
                     FDRQLMNYKKDQFDLLLEKFIAIQTENTNILNDYVHTNNQLIETLIGGI"
     gene            2916..3332
                     /locus_tag="ACG8KR_002740"
     CDS             2916..3332
                     /locus_tag="ACG8KR_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0202049.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDVNNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIMAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     ENQLNPSRRNQPKNRQKETNQTFTSGNFDPTNFLKR"
BASE COUNT         1463 a          678 c          629 g         1048 t
ORIGIN      
        1 aaccgtcagg tttagctaag tggtgagtag ttcacaaaag tcggcacacg agcaaatatc
       61 tttatcaagc tctaatttgc gttttaagac gtttgaaacg tctaagtata taaatatata
      121 agagtaggtt taagagtgct taaatcgaat tttagagggc attcagacaa ctttagggtt
      181 gttagggctt gccctgaccg cctgtaaggc gctttaagaa caagataagg gatttatact
      241 aagactgtct taaaacgtct caaacgaagg ctcatagctt ctcaaagagt tccttatgaa
      301 catttttagc aaaggagccg gaagcgactg cgcagtataa tgtgagagca gagcgatcac
      361 acctttttaa gcgactgtag ggagtaagat tcccagttga gaaaaatgcg cgaatctgtt
      421 tggagaaaaa agaagtggtt ttttaggcgg gaaattttgt ccccctcttt tataatctct
      481 ttttaaacct ctttttaaac ctctttaccg tcggctatat ccttactctc ccaagggaat
      541 gacgctttta tctacatcgt tttgtctgtt tatctacatc gttttgtctg tttatctaca
      601 tcgttttgtc tgtttatcta catcgttttg tctgtttgta cattgaatta gtacatcgac
      661 gatgttaata taagatgtag ataaacaggc attttgtatt tcataacgtg tatctcttgt
      721 gcgagtaaga aaggagctat tttaaatatg tctaatgaaa tcgtaaagta tcatcatgaa
      781 ttaaatacaa ttccgttgcg aaaatttaca ccagtcgaaa tgaatttgtt cttttctatc
      841 gtatcaagaa tgcgtgatgt aggtgataaa aaagtacaat ttacattcga gcaattaaaa
      901 gatttaagca attataaagc aactgcaaat gttcgattca tagatgattt agaaacaacg
      961 tatgacaaat taatggattt acgttttggt agaagaagtg ctgacggttt gcaacgtgaa
     1021 cgttttgttt tatttaatca atttaaaatc gacggaaaat ctgatattcc ttttgctgaa
     1081 atacaagttc atgaaaaagc cttaccctta cttaataatc ttgaagaatg ggtgcgctat
     1141 tctctccaac aattcaatga attagaaagc agttattcaa aaacaatgtt tcgattacta
     1201 aaacaataca gaaccaaagg tttcgcttat ttttcaaaag aagactttca tgaattactt
     1261 gatattccta aaagttacaa ccaaggtacg attgatcaac gtgtgttcaa tccaatacga
     1321 caagaactaa ccgcaatctt taaaggttta acaatcaaaa agaaatacgg aaaaggacgg
     1381 ggaaagcctg tcattggcta tcaatttaca tttaaacctg agataaaaaa atcagacgac
     1441 tttaacaaac atgccccaac aaaagaagaa caaaacaaag cattagaact agctaaccaa
     1501 caagttcaat ctgaaaaagc acctacttca ttacaagaaa aaatcaaaga acaaaagcga
     1561 aaattggaac ttttacaaca gttagaaaag ttagaaagag aacaaaatac ccaaaataaa
     1621 gaaaataatg cacaaaaatc agagataaat tccgatattc cttcagaact aaaaaaagac
     1681 ttattagaca atcttcaaaa cttattcaaa gattgagggg tttttagatt ttatgacaga
     1741 aaaaacaatc aaagaacttg ctgaagaatt aggagttagt aaaacagcaa ttaacaaaaa
     1801 agtttctgac gaaaatcgaa aactttggtt ttcgaaaatc ggaaacaagt ttgtgattaa
     1861 tgaaaccgga caaaaggcta taaaatcaat gttttttaac gaaaacgaaa actcaaatca
     1921 acaactagtt ggcgaaaaaa cgaaaaccga aaaccacaaa aacgccaacc aagttcccga
     1981 attagaagtc ctttctctcc tagaacaaca gttaaatcaa aaagatatgc aactacaaga
     2041 aaaagacgag caaataaaac aaatgcaaaa attactcgac caccaacaac aactcaccct
     2101 gcaagccaac caacaaatcg agaaactgca ggaacaacta cagttgactt atacggaaga
     2161 atcttcggaa aataaatcaa cgactctttc tgaagaaaca gaatcgatag aaaaacaatc
     2221 aaaggcggag aaaaaatggt ggcaattctg gcaataaaac cagcaattta aattaataaa
     2281 aattgctggt tttattcttc tttttgcttt tgcgagcaga gcaacatgga cccatgttgc
     2341 gatattttgg tggcgcccca atccccgttt tttcgttgaa tcttactcgg actgcactcg
     2401 ttttacagct gatttttctg aattttgaaa gagcataagg aaacgtggta tcactttctt
     2461 acgaaaggtg ctagataggc tgtgcctatc acgtttctca gaaaaaaatt tgctatcact
     2521 tttttgccat tgtggctagg ctatgtatag tgttttggcg aaaaatagct atacaaatac
     2581 tatatttgta tggtatacat aaattatacg ctgatacgaa aggaaaattt ccatggacat
     2641 tttagttcga aatatcaatc ccaaaattat tgcaagaata gataaacgtt gtcaggaact
     2701 cacaaccaaa acgggcaaaa aatggagtcg aaataaatat ctaaaagttc taatcgaaag
     2761 tgattttgac cgtcaactta tgaattacaa aaaagatcaa ttcgatttat tactcgaaaa
     2821 atttattgcc attcaaacag aaaatacgaa catactgaat gactatgttc acacaaacaa
     2881 ccaactcatt gaaacactta tcggaggaat ataacatgag aaaaacttct gttcaaaaac
     2941 acgtaaccct ttatcccagt cacgttgacg ttttagattc catcatcgaa aaaaatccag
     3001 acgtcaacaa ttattcagaa gccattcgtt ttttatgtat gagttatgaa cctgatacca
     3061 atacaaccac aaccagtaac caagcaaaac tcaatgccat gggaaaagaa atttctatca
     3121 tgacagaaat catggcagaa tttgctgatt tatatctaaa agatactggt gttcttatcg
     3181 gtacaaattc taaagtgtat caagaagtga aagaaaaaat tgaaaatcaa ctcaatcctt
     3241 cccgacgaaa tcaacccaaa aatcgtcaaa aagaaaccaa tcaaaccttt acttctggaa
     3301 attttgaccc aacaaacttt ctaaaaagat aaaaaaagaa accgatccct aaaaaaattc
     3361 ctcgatttct ttttagggat cagtcttcca gcaagatttg caagttggta ccaacttgcg
     3421 aaaaaaaaga aaaatccgct tatgcaactt cttctttttg tattttgggg cgctacccca
     3481 atccccaagt atcggttatt taatagcgcg gaaatccaaa gaataggacg taccctgaaa
     3541 caaaaaaaga ctgctctcac gagcagccca aaatgagatt atcctattca tcaacataaa
     3601 aatattacaa agtgaagaac tagagaaaga caaaaaaagc atatcaaatc ctctcaaaaa
     3661 taacaagtct ttaaaaaatg acagataaaa atgcacaaag aactaaaaaa gagaaatcgc
     3721 gtaaaatcaa gcattagaag ctagtaacta gctagaccaa ttaaaaatca caaagtgagc
     3781 gtctctatcc attggtagag gggctacttg gcatagtg
//
LOCUS       DAZKLD010000187         3807 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.190, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000187 DAZKLD010000000
VERSION     DAZKLD010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3807
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.190"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(459..1088)
                     /gene="udk"
                     /locus_tag="ACG8KR_002741"
     CDS             complement(459..1088)
                     /gene="udk"
                     /locus_tag="ACG8KR_002741"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HFQ0202050.1"
                     /translation="MTKSKPIIIGVTGGSGSGKTSVSRAIFNNFPDHSIMMLEQDSYY
                     KDQSHLSFEERLNTNYDHPFAFDNDLLIQHVGDLLNYKAIEKPVYDYVAHTRSQATII
                     QEPKEVIILEGILILEDERLRDLMDIKVYVDTDDDIRIIRRIKRDMEERGRTLDSVIE
                     QYLTVVKPMYHQFIEPTKRYADIIVPEGGENHVAIDLITTKVASFLNHK"
     gene            complement(1172..1867)
                     /locus_tag="ACG8KR_002742"
     CDS             complement(1172..1867)
                     /locus_tag="ACG8KR_002742"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HFQ0202051.1"
                     /translation="MKYKTLLFDVDDTLLDFQLTEANALSALFAEQGITLTPEIETSY
                     KALNHHLWQEFEKGRLSREEIIGSRFGLLFQQFGKTVDSDQMEKRYRHYLNQGHDLVP
                     GSLEILKKVHPHADLYIVTNGVSHTQHRRLTDAGMLDYFKDIFVSEAVGAQKPMKEFF
                     DHVFSHIPNFDKKQTVIIGDSLTSDIKGGNMAGIDTIWFNRNKLPEIEEIQPTYRIDS
                     LEELYSLLEIEKD"
     gene            complement(1880..2596)
                     /locus_tag="ACG8KR_002743"
     CDS             complement(1880..2596)
                     /locus_tag="ACG8KR_002743"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0140098 - catalytic activity, acting on
                     RNA [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase"
                     /protein_id="HFQ0202052.1"
                     /translation="MRLDKLIEEKLETTRKEMKRLFALKQVMIDGTVEFRQNRNVDSL
                     LHQIEVAGEHLETNESYYLLNKPEKAVTAVSDPEKTTVIDLIASADRFQPLYPVGRLD
                     RDTTGLLLITSNGQLGYEMLLPEKKVSKTYQVVVNERVTPADVTAFENGIVFADGYQC
                     KPADLKILKADKHQSTVLLTIQEGKFHQVKKMFLAQGKKVTALKRLSMGPLELDEQLG
                     VGEYRSLTLAEMKKLSIYFK"
     gene            2926..3534
                     /locus_tag="ACG8KR_002744"
     CDS             2926..3534
                     /locus_tag="ACG8KR_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HFQ0202053.1"
                     /translation="MSVSLEVSKREVRPRSLRNKLRHEGKVPAVVYGYKVESTPISFD
                     GLTLSKLLREHGANTVITMDIDGQKVNTLVHKIQTNTFTNHFEHAEFLSVNMSEETEV
                     ETEVVLVGESVGAKSGGVLAQNLYTVVVSATPDKLPERIEVDVTNLELGDSITVADLP
                     KNDDYKFVTDSEEQIAAVVAQSEEEESTGEGGEPEVIGSTEE"
BASE COUNT         1088 a          777 c          674 g         1268 t
ORIGIN      
        1 gggggtgcga caaaagagct tcttcaaaaa aataagtcga aatctcacac aaattcgaga
       61 aataattttc gtaagtttct tcttatttat tgcatctgac cacttctgtt acaacctctc
      121 gttaaacggt gttcaaaagc ttgcatctac gataataagc ccaacccaac gaaaaacgtg
      181 aggagctgtt ttatttggat gctcttatcg cttgatgcaa agacagcttt tttacaacct
      241 ctacttcacg gtgctcaatc agctgacctt cggtattagc tcaaaaaacg gtaaaatata
      301 aacagctatt ttccgtttct tctttttaat acttaggtca agatgctgtt ttcacaacct
      361 cttgttataa ataaaaaaac agttggctgg tgactgttca aaaagcagaa tccttgcttt
      421 tcaaacggtc gcagtccgtt caactgtttc gctgcgcact atttatggtt taagaaacta
      481 gctacttttg ttgtaatcaa atcaatagca acgtggtttt ctccgccttc tggaacaatg
      541 atatccgcat agcgcttcgt tggttcgata aattgatgat acatcggttt aacaactgtc
      601 aaatattgtt cgatcacgga gtccaatgtg cgtccccgtt cttccatatc ccgtttgata
      661 cgacgaatga tacgaatgtc atcatctgta tctacgtata ctttgatatc catcaagtca
      721 cgcagacgtt cgtcttctaa aatcaggatt ccttctagaa tgatcacttc ttttggttcc
      781 tgaatgatcg ttgcctgact tcttgtatgc gctacatagt catacacggg tttttcaatg
      841 gctttatagt tcagtaagtc gcctacatgc tgaatcagca gatcattatc aaaggcaaat
      901 gggtgatcgt aatttgtgtt caatcgttct tcaaaactca aatggctctg atccttgtaa
      961 taagaatctt gttcaagcat catgatggaa tggtccggga agttgttgaa aatcgcacgg
     1021 ctgacacttg tctttccgct tccggatcca cccgtcacac caatgataat tggcttgctt
     1081 tttgtcattg actaaaacct ctttcgtatc cgttttttca ttctaatcta ttatagcgaa
     1141 aaaacacgaa aatgctatat ttttctgaaa attaatcctt ttcaatttcc aataatgaat
     1201 aaagttcctc taatgaatcg attcgataag taggctggat ttcttcgatt tctggtaatt
     1261 tatttctatt gaaccaaatc gtatcgatcc ctgccatatt tcctcctttg atatcagaag
     1321 tcaaagaatc accaatgatc acagtttgct ttttatcaaa attaggaatg tgggaaaaga
     1381 catgatcaaa aaattctttc atcggctttt gcgctccgac agcttctgag acaaagatat
     1441 ccttgaagta atcaagcatt cctgcatctg ttaatcgccg atgttgcgtg tgcgacacac
     1501 cgttcgtcac aatataaaga tcagcatgcg gatggacctt tttcaggatt tctagacttc
     1561 ctggaacaag atcatgacct tggttcaagt agtggcggta gcgtttttcc atttgatcac
     1621 tatctactgt tttcccaaat tgctggaaaa gtaacccaaa acgactgcct attatttctt
     1681 ctcgtgatag tctgcctttt tcaaactcct gccataaatg atgattcaaa gccttatagc
     1741 tggtttcgat ttctggtgtc aatgtgattc cttgttctgc aaacaaagca cttaaagcat
     1801 tggcttcagt taattgaaaa tctaacaatg tatcatctac atcaaacaat aaagttttat
     1861 atttcatctt tttgacctcc tatttaaaat aaatacttaa ctttttcatc tctgctaatg
     1921 tcaacgatcg atactcgcct actcctagct gttcatctaa ttccaatggt cccatgctta
     1981 gccgtttcaa tgcggtcact ttctttcctt gggctaagaa catctttttg acttgatgga
     2041 attttccttc ttggatcgtc aataaaaccg tactttggtg cttgtctgct ttgagtattt
     2101 ttaaatcagc cggcttacat tgatacccat cagcgaatac gattccattt tcaaatgctg
     2161 ttacgtctgc cggtgtcacc ctttcgttca caactacttg ataagtcttt gaaacctttt
     2221 tttctggaag tagcatttca tatcccaatt gtccattcga agtgatcagc aatagtcctg
     2281 ttgtatctct atctagtctt cccactggat ataatggttg gaaacgatcg gcacttgcaa
     2341 tcagatcgat tactgtcgtc ttttctggat ctgatacagc tgtaacagct ttttcaggtt
     2401 tattgagaag ataataggac tcatttgttt ctaagtgttc acctgctact tcgatttgat
     2461 gcagtaaact atctacattt cgattttgtc gaaattcgac tgtgccatct atcatcactt
     2521 gcttcaaagc aaacagccgc ttcatttcct tgcgtgtcgt ttctaatttt tcttctatta
     2581 atttatctaa acgcatgctg tttcctttct tattactact aattaattat accttgaata
     2641 ttcttgcttg caaatacatc atcatagtta ttccttgact taattaacag ttcacgttac
     2701 ggttaaatag taaagtgaat tggatttctc atacacagcg taatgacctg gtggagaaaa
     2761 gttgaatcct gactttctaa ggggagatgc gcgcatctct ccttttgcgc ttttttaagc
     2821 gccctgcctt cgatgatgtg cacgagatta gcctttagaa ttcacttaaa taaaatctgt
     2881 ccgttaccaa agcagacagt cacattttaa ggaggaattt tggttatgtc agtatcatta
     2941 gaagtaagta aaagagaagt acgtccccgt tcattaagaa acaaacttcg tcacgaaggg
     3001 aaagttccag ctgttgttta tgggtataaa gtagaaagta cccctatctc attcgatggt
     3061 ttgacactaa gcaaattgtt gcgtgaacat ggagcgaaca cagtcatcac gatggacatc
     3121 gatggacaaa aagtcaatac attggttcac aagatccaaa cgaacacttt taccaatcat
     3181 ttcgaacatg cagaatttct atctgtaaac atgtcggaag aaacagaagt cgaaacagaa
     3241 gtcgttcttg ttggcgaatc agtgggtgct aaatctggtg gagtgttagc tcaaaacttg
     3301 tatacagtag tcgtatctgc tacaccagac aaattgcctg aacgaattga agttgacgtg
     3361 acaaacttgg aattaggtga ttctattaca gtagccgatc ttccaaaaaa cgacgattac
     3421 aaattcgtga ccgacagtga agagcaaatc gctgctgtag tcgctcaaag tgaagaagaa
     3481 gaaagcacag gtgaaggtgg cgaaccagaa gtgatcggtt caacagaaga ataatactga
     3541 aacaatcagg tggtgttaga agcggcgggt cagcagcttt taacacgcct tttttatttc
     3601 taatcgttta ttttcatatg gggcttccac ttttgtaaat ggctttacaa acgaaaaatc
     3661 ctaatttacc aagcttttcc tcgcatactt ttatcgtacc tttataaaaa tttatacttt
     3721 gaaaaaaagt attcaattat tgttgcattt ttcctgattc tttattagaa tgaaccctcg
     3781 ttgaagtaag tatttctagt ttatcct
//
LOCUS       DAZKLD010000188         3758 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.191, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000188 DAZKLD010000000
VERSION     DAZKLD010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3758)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3758)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3758
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.191"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(280..513)
                     /locus_tag="ACG8KR_002745"
     CDS             complement(280..513)
                     /locus_tag="ACG8KR_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HFQ0202054.1"
                     /translation="MVQIKTTTILIYLLAIQELSKRKKKLKNNDLAKVLSVSPASVSE
                     MLAKLRYEDYLDIGFQLTDKGKKFIDDYKKGFK"
     gene            complement(528..1478)
                     /locus_tag="ACG8KR_002746"
     CDS             complement(528..1478)
                     /locus_tag="ACG8KR_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding
                     lipoprotein"
                     /protein_id="HFQ0202055.1"
                     /translation="MMRKWKAVLGSLGILIALFIFGACSINSKDKDKVASNEKLKVVV
                     TNSILADITENIAKDKIDLHSIVPIGKDPHEYEPLPEDVQKTSKADLIFYNGVNLETG
                     GNAWFTKLVKNANKEENKDYFAASDGIDVIYLEGQSEKGKEDPHAWLNLENGIIYAKN
                     IEKQLAEKDPDNKKFYKENLDKYIEKLDSLDKEAKSKFASIPNDKKMIVTSEGCFKYF
                     SKAYNVPSAYIWEINTEEEGTPDQIKHLVEKLRTTKVPSLFVESSVDDRPMKTVSKDT
                     NIPIYSTIFTDSIAEKGQDGDSYYAMMKWNLDKIAEGLSK"
     gene            complement(1475..2317)
                     /locus_tag="ACG8KR_002747"
     CDS             complement(1475..2317)
                     /locus_tag="ACG8KR_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease subunit"
                     /protein_id="HFQ0202056.1"
                     /translation="MLQNFINGLYDFHFLQNALITSVVIGIVSGTVGCFIILRGMSLM
                     GDAISHAVLPGVAISYILGINFFIGAIVFGLLASIIITFIKSNSVIKGDTAIGITFSS
                     FLALGVILIGVANSSTDLFHILFGNILAVQDIDKWITIGVSVLVLVIVVLFFKELLIT
                     SFDPLMAKAIGMNVSFYHYLLMVLLTLVAVTAMQSVGTILIVAMLITPAATAYLYANS
                     LKTMIFISATVGASSSLLGLFIGYSFNIAAGSSIVLTAAFLFVISFLISPKQKFLRKK
                     ERSL"
     gene            complement(2319..3038)
                     /locus_tag="ACG8KR_002748"
     CDS             complement(2319..3038)
                     /locus_tag="ACG8KR_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HFQ0202057.1"
                     /translation="MLEVKKLNVCYNDFLALNEISLKIQEGALTGIIGPNGAGKSTLL
                     KAMLNIIPHQGEVSINNEDINKKLVKIAYVEQKADIDFTFPIKVKECVSMGTYAGMKV
                     FQRIKNAEWQRVSKALEKVDMGKYSNHQIGELSGGQFQRVLLARCLAQNADFIFLDEP
                     FVGIDLVSERIIMDTLKKLKHQGKTVLIVHHDLSKVKKYFDNIILLNRNLIAHGSVES
                     VFNEENLKKAYGDTIFIGEGE"
BASE COUNT         1274 a          686 c          466 g         1332 t
ORIGIN      
        1 ctaataagat gaattaactt tattatctta cacaggactt gaagtttcaa tcgttaacgt
       61 ttattactga actcattcct tagagctaaa ctaatgagaa agagcagaat aactgataca
      121 aaaagcaatc ctacaagaat taatagactc atctaaatca ttcacctttt ctaaaataat
      181 ccgagaaaga tttaaaaaaa ttatatccag ctattaggat aagtcctatc ataatcactc
      241 caatagccat tttcttatcc tccctaaaaa taaagtcttt tacttaaaac cttttttgta
      301 atcgtcaata aattttttcc ctttatcagt taattgaaat cctatatcta aatagtcctc
      361 atatctaagt ttagccagca tctcacttac agatgctgga ctaacactta gaacttttgc
      421 taaatcatta ttttttaatt tcttttttct ttttgataat tcctgaattg ctagtaaata
      481 aattagtata gtagtcgttt taatttgtac catacctacc ctccaaatta tttcgaaagg
      541 ccttcagcaa ttttatccag gttccatttc atcatcgcat agtaactatc accatcttgt
      601 cctttttctg caattgaatc agtaaaaatc gttgaataga taggaatatt ggtatctttt
      661 gatactgttt tcattggtct atcatccaca ctgctttcta cgaataagga gggaactttt
      721 gttgtgcgta atttttcaac taagtgtttt atttgatctg gtgttccttc ttcttcagtg
      781 ttgatttccc aaatgtaagc agaaggcaca ttatacgctt tcgagaaata tttaaagcat
      841 ccttcacttg taacaatcat ttttttatca ttcggaattg aagcaaattt agatttagct
      901 tctttgtcta gagaatccaa tttttcaata tacttatcta gattttcttt atagaatttt
      961 ttattatcag gatctttttc cgctaactgt ttttcaatat ttttagcgta aataataccg
     1021 ttttctaaat ttaaccaagc atgaggatct tccttccctt tctcactctg accctctaag
     1081 taaataacat ctatgccatc acttgctgca aaatagtctt tgttttcctc tttgttcgca
     1141 tttttaacta attttgtaaa ccaagcattt cctccagttt ccaagttaac accgttataa
     1201 aaaatcaaat ctgcttttga agttttttga acatcttcag gcaaaggttc atattcgtgg
     1261 ggatctttcc caataggtac gatactgtgt aaatcaattt tatcttttgc tatattttcg
     1321 gtaatatctg ctaaaatcga attagtaact actactttta atttttcgtt cgaagccact
     1381 ttatctttgt ctttactatt tattgaacat gccccgaata taaaaagagc aatcaaaatc
     1441 cccagacttc ccagtactgc tttccatttt ctcatcataa cgatctctcc ttctttctta
     1501 aaaatttttg ttttggtgag attagaaaac taattacaaa taagaatgcc gctgttaaaa
     1561 caatacttga tcctgctgct atgttaaaac tataaccaat aaacaatcca agcaatgatg
     1621 aacttgctcc gactgttgca gaaataaata tcatagtttt caagctattt gcatataaat
     1681 atgctgtagc tgctggtgta attaacattg caacaattaa tatagttccc acactttgca
     1741 tagctgttac tgctactaaa gttaatagaa ccatcaatag ataatgataa aaactgacat
     1801 tcatccctat tgcttttgcc attaatggat caaaagaagt gattaataat tctttaaaaa
     1861 atagaactac tataaccaac actaaaaccg aaacgccaat tgtaatccac ttatcaatat
     1921 cttgtacagc taaaatattt ccaaataaaa tatgaaataa atccgttgag ctatttgcaa
     1981 ctcctataag gataactcct agtgctaaaa aagaactgaa agtaatacca attgctgtat
     2041 ctccttttat tacactattg ctttttataa aagtaataat gatagaagct aatagaccaa
     2101 atacaatcgc accaataaaa aaattaatac ctaaaatata agaaattgct actcctggca
     2161 aaacagcatg agaaatagca tcacccataa gagacattcc tcttaaaata ataaaacaac
     2221 ctactgttcc agaaacaatc cctataacta cagatgtaat tagagcattc tgtaaaaagt
     2281 gaaagtcata cagaccatta ataaaatttt gaagcatatt attccccctc tccaataaat
     2341 atagtatctc cataggcttt ttttaaattt tcttcattaa atacagattc tacacttccg
     2401 tgagcaatta gatttcgatt caaaagaata atattatcaa aatacttctt taccttgctt
     2461 aaatcatgat gcacaattag cactgttttt ccctgatgtt tcaatttttt taacgtatcc
     2521 ataattattc gttcactaac taagtctatc cctacaaatg gttcatctaa aaatatgaaa
     2581 tccgcatttt gagcaagaca tcttgctaaa agtacacgtt gaaattgtcc tccagataat
     2641 tctcctattt gatgattaga atattttccc atatcgacct tttccaaagc ctttgaaact
     2701 ctttgccatt ctgcattttt aattctttga aaaactttca ttccggcata tgttcccata
     2761 gatacacatt cttttacttt tattggaaaa gtaaaatcaa tatcagcttt ttgctctaca
     2821 tatgcaatct tcaccagttt tttattgata tcttcattat taatggagac ctctccctga
     2881 tgagggataa tatttaacat tgctttaagc aatgtggatt ttccagcacc atttggacca
     2941 ataatacctg ttaaagcacc ttcttgtatt tttaaagaaa tctcgttcaa tgctaaaaaa
     3001 tcgttataac aaacattaag ctttttcact tctaacataa ctttctcctt ctaaatgata
     3061 atgattatca cgatcatatg gtatacaaaa attatatttt agtcaaccct aaattttata
     3121 gtttagattt ttttctgttg ctgaagatta gaatgacaaa actatttgaa actacgcgta
     3181 tagtaagtag ctttcaaaat atataaaaaa ggagcgttct caaatgagaa catgctccta
     3241 aaactacttt attggcgtat ggaaaaatta tacttgaagt ggatagcggg ttgaataagt
     3301 tgataagcat caaaacattt ataaaaatta ttcaagacta tgtctgttcc atatacaaat
     3361 atgaaaatca gtaaactcca tttaactttt ttcgtagtgt gtctttttat acatgtttat
     3421 cgttattaat agtgaatagg aaaaaaacta tttttgatca ttgacaatta aatacaattt
     3481 ttgaataacg atccctgcta ttcgagctag aaactcaagt acgccatgac ctgattagcg
     3541 acaaatactt agagatttct ttttgttctg ggaaaacaaa atgccatgac aatagcagca
     3601 aataatgata cttccgtaag gctcgccgta aactggggag aggtgaaatt cctatgtgct
     3661 tacccaaagc aattcaaaaa tttcataact actattttag gagatgattt tatagaatcc
     3721 agagtctacc tgaataaaaa acaaacctgt gcatttct
//
LOCUS       DAZKLD010000189         3682 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.193, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000189 DAZKLD010000000
VERSION     DAZKLD010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3682)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3682)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3682
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.193"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            14..1141
                     /locus_tag="ACG8KR_002749"
     CDS             14..1141
                     /locus_tag="ACG8KR_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFQ0202058.1"
                     /translation="MAFLFVSLNYTNGEDINDDGSRKKVEKVGGDTRPEAEAALRKVL
                     SDIDETGQYFLGTDTRVKQYLDFWMEEYVKLNLKYNTYENYRFTIKNHINGYLGKKKL
                     TDLSPALLQNFINAEFKKGYSKKTMTITHSVLKNALNMAVYPWGLIKQNPMLYVKIPK
                     YEARPTTKKDLKIISLEDFDHMLEITPEGHPFYIPLNIGFYTGMRVGEVCGLTWDDVD
                     FSNGTITVEKQMVKNDGAWVYGTPKTSSSNRTIFIGQTLLAILKKHKKQQLENRMKYG
                     KLYIDSNAVCTKEDGELVTPSVVKWNTRRISNALSLSFNFHSLRHTHATLLLENGAKM
                     KEISERLGHSRISITMDTYSHVTDKMRNETVDIMENLRKNS"
     gene            complement(1359..2501)
                     /locus_tag="ACG8KR_002750"
     CDS             complement(1359..2501)
                     /locus_tag="ACG8KR_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFQ0202059.1"
                     /translation="MRKIANVYKDRKRGTWFFSATLGQDRFGKRIQVTRRGFKTQGEA
                     RRGLEELKQSFSDSQGIYLQGITFHDFYREYFFPWYKLGTVEKTYMKTDHILQRALDF
                     FGEMQVVKIRPIHIQEFQQFLSKIHVVKKGIEQPLSPNYIKQIFNKLRVVFKRAIVLE
                     IIEENPVDTIGKIKTQRPQVEFWTVDEFKKVYNSVYHGDFYEEFYKRLMRFIFVTGVR
                     SEELLALQWKDFDLINGRCSVNKSIYIRTRQDYEFAETKNTSSIRIISLDRQTVEDLK
                     TWKKTQQSIGKNIELVFSFDGLPPSPRTILSRIKKLAEIAGVKPIHMHGLRHSHVAFL
                     IEHNKNIYAISKRLGHASIKTTLDKYGHLYPETNQALANEITRFDI"
     gene            complement(2506..2691)
                     /locus_tag="ACG8KR_002751"
     CDS             complement(2506..2691)
                     /locus_tag="ACG8KR_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HFQ0202060.1"
                     /translation="MKTATRKDLINLGFSQYYANKIFKECKELLVEKGYFFYRNSKIK
                     RIPISILEEVLHISWEE"
     gene            complement(3045..3290)
                     /locus_tag="ACG8KR_002752"
     CDS             complement(3045..3290)
                     /locus_tag="ACG8KR_002752"
                     /inference="COORDINATES: protein motif:HMM:NF024059.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0202061.1"
                     /translation="MVDKSKKRQYLLVPLSIILSALEGDPISIKMILKHYHSYMAKLC
                     LTNGFNEVGQFVTYVDEYMISQLEIKLIEAILKFKIN"
     gene            complement(3284..>3682)
                     /locus_tag="ACG8KR_002753"
     CDS             complement(3284..>3682)
                     /locus_tag="ACG8KR_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010818872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HFQ0202062.1"
                     /translation="QQHYNDISIQHQFDAYCKKVLRNEAKSIRKRNANIKDNEEPLAD
                     SNEGRYSQTHFDEQDVYFLFGMEILISDQKLSAAIDQLSEIRRKIILLYYFAGFNDAE
                     IGKILNMSTSGIWYQRKKAVEQLKMGYDLW"
BASE COUNT         1204 a          660 c          570 g         1248 t
ORIGIN      
        1 gagtgtaaca taaatggctt ttttatttgt ctcgcttaat tatacaaacg gcgaagatat
       61 caatgatgat ggctcaagaa aaaaagtgga gaaagttggc ggagacaccc gaccagaggc
      121 cgaagctgct ttacgaaaag ttttatcaga tattgacgaa acaggacaat actttttagg
      181 tacggatact cgagtaaaac aataccttga tttttggatg gaggaatacg ttaaactaaa
      241 tctaaaatac aatacctatg aaaactaccg atttaccatc aaaaatcata taaacggtta
      301 tttaggaaag aaaaaactta cggatctctc ccctgctctt ttacaaaatt tcatcaatgc
      361 tgaatttaaa aagggttact cgaagaaaac aatgactatt actcactctg tccttaagaa
      421 tgcgctgaat atggcggttt atccttgggg attaatcaag caaaatccta tgctgtatgt
      481 aaaaatacca aaatatgaag cacgaccaac gactaaaaaa gatctaaaaa tcatttctct
      541 tgaggacttt gatcatatgc tagaaattac tcctgaagga catcctttct atattccttt
      601 gaatattgga ttttatacgg gaatgcgcgt tggcgaagtt tgtggtctga cgtgggatga
      661 tgtcgatttt tcaaatggaa caattactgt agagaaacaa atggtaaaga atgacggcgc
      721 atgggtatat ggtacgccaa agacaagcag ttccaatcga acgattttta ttggacaaac
      781 cttgctagca attctgaaaa aacataagaa acaacaatta gaaaatcgaa tgaagtatgg
      841 aaagctctac attgattcaa atgcagtatg tacgaaggaa gacggtgagc tagttacgcc
      901 aagtgtagtg aaatggaata ctaggaggat atcgaatgca ctctccctct cttttaactt
      961 ccattctctc agacatactc atgctacact tcttctcgaa aatggcgcaa aaatgaaaga
     1021 aatctctgaa cgattgggcc acagcagaat ttcaattacg atggatactt actcgcatgt
     1081 aacagataag atgagaaatg aaacggtcga tatcatggaa aatcttagaa agaattcttg
     1141 atttttgcca ccgaaaaaaa ccaccggtgg caaaccggtg gcaaatccat gtaaacatgg
     1201 tttatttttt acactgtttt caaacaaacc cttgctattt cttcggttcg tcctcgtcca
     1261 ttttcgatac cgacagctag cttataaccc aaggtatatc aacataaaaa aataaaaata
     1321 ggctatgaca gcagcgtgac agcaaaaaag atggaaagtt aaatatcaaa cctcgtaatt
     1381 tcatttgcga gtgcttgatt tgtttctgga tataagtgtc catatttgtc caaagtagtt
     1441 ttaatagagg catgacctag acgcttagat attgcataga tatttttatt atgttcaatt
     1501 aaaaaggcaa catgcgagtg tcttaatcca tgcatatgaa ttggttttac tcctgcaatt
     1561 tcagctagtt tttttattct gcttaaaata gttcttggag atggcggtaa tccatcaaaa
     1621 gaaaaaacca attctatatt tttcccaatt gattgttgag tttttttcca tgtttttaaa
     1681 tcttcaacgg tttgtctgtc taacgaaatt atccgaatac ttgatgtatt ttttgtttca
     1741 gcaaattcat aatcttgtct tgtacggatg taaattgact tatttacgct acatctgcca
     1801 tttattaagt caaagtcttt ccattgtaac gctaagagct cctcactcct tacacctgta
     1861 acaaaaataa aacgcatcaa acgtttataa aattcttcat aaaaatcccc gtgataaaca
     1921 ctattatata ctttcttaaa ttcatcaact gtccaaaatt ctacttgagg acgctgagtt
     1981 tttattttac ctatagtatc tacaggattt tcttcaataa tttcaagtac aattgccctt
     2041 ttaaaaacta ctctcaactt attgaatatc tgtttaatat aatttggtga taatggttgc
     2101 tcaatgccct tttttacaac atggattttc gataaaaact gttgaaattc ctgtatatga
     2161 attggtcgaa tctttacaac ctgcatttct ccaaaaaaat ccaatgcacg ttgtaaaata
     2221 tgatctgttt tcatatatgt tttttctaca gttcccaact tataccaagg aaaaaaatac
     2281 tctcgataaa aatcatgaaa cgttattcct tgcagataaa ttccttgtga atcagaaaaa
     2341 ctttgtttta actcttcgag tccccttcta gcttctcctt gagttttgaa gcctcttctg
     2401 gtcacttgaa ttctttttcc aaagcgatct tgtcctaaag ttgcagaaaa aaaccaagtg
     2461 cctctttttc tgtctttata aacattcgca attttcctca ttttactatt cctcccaaga
     2521 aatatggaga acttcttcta aaattgaaat aggaattctt tttatttttg aattacgata
     2581 aaaaaagtat cctttctcaa ctaaaagttc tttacattcc ttgaagattt tattagcata
     2641 gtactgagaa aaacctagat taatcaaatc ttttcgagta gctgttttca ttatagcacc
     2701 ttccttactt tttttcattt atttctatca gctgcttttg tcaaaagcac ataggaattt
     2761 cacctctccc caagtttacg gcgagcctta cggaagtatc attatcccaa ctttaaatca
     2821 tcgcttttta ttgactataa aaattatttt agaatattta tcgctccttc tttacagcaa
     2881 gaaatcatgg cgcaactaaa atatgctcct taaagtgtta acgtactgat agaatctatt
     2941 taattttcaa tgaacaatag gttttctacc taaaagaatc tactaattcc ttaactatta
     3001 gaagattctt ttaaacagaa aaagtggaaa ggggattttt ttaattagtt aattttaaat
     3061 tttaagatcg cttctattaa tttaatttct agctgactta tcatatattc atctacatag
     3121 gtaacgaatt gacctacctc gttaaatcca tttgtcaaac ataacttagc catataagaa
     3181 tgataatgtt tgagtatcat cttgatcgaa attggatctc cttctagagc agataagata
     3241 atggatagtg gaactaatag gtactgccgc tttttagatt tatctaccat aggtcatacc
     3301 ccattttcaa ttgttcaact gccttctttc gttgatacca gataccactt gtgctcatat
     3361 tgagaatttt accgatttca gcatcattaa aacctgcaaa ataataaagt aggataatct
     3421 ttcttcttat ctccgataac tgatcaattg ctgctgataa cttttgatct gaaattaata
     3481 tttccattcc aaataagaaa tagacatctt gttcatcaaa atgagtttgg gaatacctgc
     3541 cttcatttga atctgccaat ggttcctcat tatcctttat gttagcgttt cgttttcgaa
     3601 tagacttagc ttcgtttcgt aatacttttt tacagtaggc atcaaattgg tgttgtatag
     3661 atatgtcatt gtaatgttgt tg
//
LOCUS       DAZKLD010000190         3605 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.194, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000190 DAZKLD010000000
VERSION     DAZKLD010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3605)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3605)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3605
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.194"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..189
                     /locus_tag="ACG8KR_002754"
     CDS             <1..189
                     /locus_tag="ACG8KR_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFQ0202063.1"
                     /translation="LVEDASLSGENERINELIEFIRKNKFRTLEYDDKLVRKIIQSVT
                     VYEGHFVIAFKSGIEMEI"
     gene            332..556
                     /locus_tag="ACG8KR_002755"
     CDS             332..556
                     /locus_tag="ACG8KR_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018579902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0202064.1"
                     /translation="MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLD
                     ELKAIADVLGVKFVQAFILPDGEEIKISNE"
     gene            571..1440
                     /locus_tag="ACG8KR_002756"
     CDS             571..1440
                     /locus_tag="ACG8KR_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HFQ0202065.1"
                     /translation="MVGNVIQIVTEKLSSLPFIEGIVLGGSRARGTHTENSDIDIGIY
                     YNSDSFDLTAINQIATELDDKNRNNLVVPPGAWGDWINGGGWLFINGYHVDLILRDIK
                     RVEQIIKDTEQGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAEIY
                     PTALKKSLMNFFIFEAEFSLMFVKANAGVEDKYYIAGHVFRIISCLNQVLFACNNAYC
                     INEKKAIKLLETFEYKPEKYAERVNHIFEVLGLSLFECYDMTEKLYKEVNEIVSEINN
                     FLNEESSDERKQI"
     gene            1421..2155
                     /locus_tag="ACG8KR_002757"
     CDS             1421..2155
                     /locus_tag="ACG8KR_002757"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008425 -
                     2-polyprenyl-6-methoxy-1,4-benzoquinone methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0061542 - 3-demethylubiquinol-n
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0102208 - 2-polyprenyl-6-hydroxyphenol
                     methylase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFQ0202066.1"
                     /translation="MKENKYDDNIFFQKYSQMSRSQKGLAGAGEWETLKKMLPDFKGK
                     RVLDLGCGYGWHCIYAMENGASSVVGVDISHKMLEVAKGKTHFPQIEYECCAIEDVDF
                     PEESFDVILSSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNE
                     KGEILHFPVDNYYYEGKRTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPE
                     NMMDIPGMADEMRRPMMLIVSAKKKM"
     gene            2188..3096
                     /locus_tag="ACG8KR_002758"
     CDS             2188..3096
                     /locus_tag="ACG8KR_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(6)-Ia"
                     /protein_id="HFQ0202067.1"
                     /translation="MRSEKEMMDLVLSLAEQDERIRIVTLEGSRANINIPKDEFQDYD
                     ITYFVSDIEPFISNDDWLNQFGNIIMMQKPEDMELFPPEEKGFSYLMLFDDYNKIDLT
                     LLPLEELDNYLKGDKLIKVLIDKDCRIKRDIVPTDIDYHVRKPSAREYDDCCNEFWNV
                     TPYVIKGLCRKEILFAIDHFNQIVRHELLRMISWKVGIETGFKLSVGKNYKFIERYIS
                     EDLWEKLLSTYRMDSYENIWEALFLCHQLFRAVSGEVAERLHYAYPEYDRNITKYTRD
                     MYKKYTGKTGCLDSTYAADIEERREQ"
     gene            3093..3573
                     /locus_tag="ACG8KR_002759"
                     /pseudo
     CDS             3093..3573
                     /locus_tag="ACG8KR_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
BASE COUNT         1252 a          521 c          789 g         1043 t
ORIGIN      
        1 ttagtagaag atgcctccct cagtggcgag aacgagcgaa tcaatgagct gattgaattt
       61 atccgcaaga acaaattccg caccttagaa tatgatgata agcttgtaag gaagataatc
      121 cagagcgtta cagtctatga aggccacttc gtcatagcct tcaaatctgg catcgaaatg
      181 gaaatatgaa aaccagaagt aaatagccca tgactctaca gtagagttgt gggttttctt
      241 ttgcttgctg attataataa tattttaata actattgtgt ttatcaacca aatggtttat
      301 aatattctta gcgtaaactt ggaggtgctg tatgactacg gcagaaatga ttaaagaact
      361 gtgtgagcaa atgaatataa gtgtttccga acttgctaga cgtattggcc agactccaca
      421 gaatttcaat aaaaaattac aacgagaaac agtaaccttg gatgagttga aggccattgc
      481 tgatgtgcta ggtgtcaagt ttgtgcaggc atttatttta ccagatggag aagaaataaa
      541 aatatctaac gaataaagga ggatgtttat atggtaggta atgttattca aatagtaaca
      601 gagaaattat catctttgcc ttttatagaa ggcatcgtat tagggggctc acgtgcaaga
      661 ggcacccata cagaaaattc tgatatagat atcggaatct attacaattc agactcattt
      721 gacctgacag cgattaatca aattgctaca gaattggatg ataaaaatag aaacaacctt
      781 gttgtgcctc ccggagcgtg gggtgattgg attaatggcg gcggatggtt atttataaat
      841 gggtatcatg ttgacttgat tttacgtgat ataaaacggg tagaacaaat aatcaaagat
      901 acggagcaag gaattgttac tgccaattat cagaccgggc atccccatgg ttatataagt
      961 gcaatgtatc gtggagaatt agcgattagt aaaatactat atgctaagaa cgaaagctta
     1021 tgcgaattaa aaaagcaggc agaaatttac cctactgctc taaagaaaag cttgatgaac
     1081 ttttttatat ttgaagctga attctcttta atgtttgtaa aagcaaatgc gggggtagag
     1141 gataaatatt atattgcagg ccatgttttt cgtattattt catgcttaaa tcaagtacta
     1201 tttgcatgta ataatgctta ttgcattaac gaaaagaaag ctataaaact gcttgaaact
     1261 tttgaatata aacctgaaaa atatgccgaa agagttaatc atatttttga agtactcggt
     1321 ctttctcttt ttgaatgcta cgatatgacc gagaaacttt ataaagaagt gaatgaaatt
     1381 gtatcggaga taaataactt tttaaacgag gagagttcag atgaaagaaa acaaatatga
     1441 tgataatata ttttttcaaa aatacagtca aatgagtcgc tcgcagaaag gactggctgg
     1501 tgcgggagaa tgggagactt tgaaaaagat gctacctgat tttaagggta agcgtgtgct
     1561 tgatttagga tgcggctatg gatggcactg tatatatgcg atggaaaacg gtgcttcctc
     1621 tgtagtaggt gttgatattt ctcataaaat gctcgaagta gcaaaaggaa aaacccattt
     1681 tccacagatt gaatatgaat gctgtgccat agaagatgtg gatttcccag aggagagctt
     1741 tgatgtaata ctaagttcgc ttgcgtttca ttatgtagca gactatgaga atttaataaa
     1801 aaagatatat aggatgctga aggctggtgg caatttagtt tttacagttg aacatcctgt
     1861 ttttactgct catggaacac aagactggta ttataacgaa aaaggagaaa tactgcattt
     1921 cccggtggac aattattatt atgagggcaa acggacagct atgtttttgg aagaaaaggt
     1981 tacaaaatat catagaacac tgaccacata tctaaataca ctgctttcaa atagttttat
     2041 aataaatcag attgtggagc cacagccgcc agagaacatg atggatattc cggggatggc
     2101 ggatgaaatg cgacgcccaa tgatgctgat tgtatcggca aaaaagaaga tgtaataata
     2161 tagaaaaaat aaacgaggag tatgtaaatg agatcagaaa aagaaatgat ggatttagta
     2221 ctttctttag cagaacagga tgaacgtatt cgaattgtga cccttgaggg gtcacgcgca
     2281 aatattaata tacctaaaga tgaatttcag gattatgata ttacatattt tgtaagtgat
     2341 atagaaccgt ttatatctaa tgatgactgg cttaatcaat ttgggaatat aataatgatg
     2401 caaaagccgg aggatatgga attattccca cctgaagaaa agggattttc ctatcttatg
     2461 ctatttgatg attacaataa aattgatctt accttattgc ccttggaaga gttagataat
     2521 tacctaaagg gcgataaatt aataaaggtt ctaattgata aagattgtag aattaaaagg
     2581 gacatagttc cgactgatat agattatcat gtaagaaagc caagcgcaag ggagtatgat
     2641 gattgctgca atgaattttg gaatgtaaca ccttatgtta ttaaaggatt gtgccgtaag
     2701 gaaattttat ttgctattga tcattttaat cagattgttc gccatgagct gctgagaatg
     2761 atatcatgga aggtcggcat cgaaacaggc tttaaattaa gtgtaggcaa gaactataag
     2821 tttattgaaa ggtatatatc cgaggatttg tgggagaaac ttttgtccac ctaccggatg
     2881 gattcctatg aaaacatatg ggaagcatta tttctatgcc atcaattgtt cagggcggta
     2941 tccggtgagg tggcggaaag gcttcattat gcctatccgg agtatgatag gaatataaca
     3001 aaatatacca gggacatgta taaaaaatac actggtaaaa ccggctgcct ggatagcaca
     3061 tatgccgctg atatagaaga gaggcgggaa cagtgattac agaaatgaaa gcagggcacc
     3121 tgaaagatat cgataaaccc agcgaaccat ttgaggtgat aggtaagatt ataccgaggt
     3181 atgaaaacga gaattggacc tttacagaat tactctatga agcgccatat ttaaaaagct
     3241 accaagacga agaggatgaa gaggatgagg aggcagattg ccttgaatat attgacaata
     3301 ctgataagat aatatatctt ttatatagaa gatatcgccg tatgtaagga tttcaggggg
     3361 caaggcatag gcagcgcgct tatcaatata tctatagaat gggcaaagca taaaaacttg
     3421 catggactaa tgcttgaaac ccaggacaat aaccttatag cttgtaaatt ctatcataat
     3481 tgtggtttca aaatcggctc cgtcgatact atgttatacg ccaactttga aaacaacttt
     3541 gaaaaagctg ttttctggta tttaaggttt tagaatgcaa ggaacagtga attggagttc
     3601 gtctt
//
LOCUS       DAZKLD010000191         3574 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.195, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000191 DAZKLD010000000
VERSION     DAZKLD010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3574)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3574)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3574
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.195"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            47..1036
                     /gene="galE"
                     /locus_tag="ACG8KR_002760"
     CDS             47..1036
                     /gene="galE"
                     /locus_tag="ACG8KR_002760"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HFQ0202068.1"
                     /translation="MTILVLGGAGYIGSHAVDQLVQKGYQVAVVDNLLTGHKQAVHPD
                     AHFYEGDIRDKEFLRSVFEKEPIEGVIHFAASSLVGESVEKPLMYFNNNVYGMQILLE
                     VMHEFNVNKIVFSSTAATYGEPKESPITEDTPANPKNPYGESKLMMEKMMKWCDQAYG
                     MRYVALRYFNVAGAKADASIGEDHTPETHLVPIILQVALGQRKALAVYGDDYDTPDGT
                     CIRDYVQVEDLIAAHILALEYLKEGNESNFFNLGSSKGYSVKEMLEAAREVTGKEIPA
                     EIAPRRAGDPSRLVASSEKAREVLGWKPEYTDIKAIIKTAWDWHVSHPNGYEE"
     gene            1048..2532
                     /gene="galT"
                     /locus_tag="ACG8KR_002761"
     CDS             1048..2532
                     /gene="galT"
                     /locus_tag="ACG8KR_002761"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0017103 - UTP:galactose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019388 - galactose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HFQ0202069.1"
                     /translation="MSISQTICDFATLAIEAGGWMEMDRLYLQNRLLGMVGEDALESI
                     EVRPVKEESVDLLDQLVAKARENGVLSELPAEQEIFEAQLMDFLTPPPSVVNAFFAQH
                     YSKSPEEATEYFFNLSKKNDYIKTRAIAKNIKFPAESEYGELEITINLSKPEKDPKQI
                     AAEREAVKVDYPTCMLCMENEGYRGRLNYPARTNHRIIRMNLDGESWGFQYSPYAYYN
                     EHSIILSEQHRPMKITKETFARLLRITEVLPHYFVGSNADLPIVGGSILSHDHYQAGR
                     HEFPMAKAPIEKLIKLADYPQVIAGVVKWPMSVIRLQSTEKKLLIEAANSVLEKWKAY
                     SDETVSIRAFSADGTPHHTITPIARRRDNYFELDLVLRDNNVSEEHPDGIFHPHKDVQ
                     HIKKENIGLIEVMGLAVLPPRLKPELVEVEHFLLGEENQMAEYHRPWAEQLKKEHPDL
                     TKDTVTDVVQKSVGQVFARVLEDAGVFKRDEKGQAAFERFTAIL"
     gene            2556..3536
                     /locus_tag="ACG8KR_002762"
     CDS             2556..3536
                     /locus_tag="ACG8KR_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0202070.1"
                     /translation="MATIKDIAQLAGVSPATVSRVLNYDPDLSVGLATKQKIFEAAER
                     LNYTKHRKNAANTKQTLRLIQWYDDTEELEDLYYLSIRLGIEKKAEEANVTLLKETWD
                     SISDLPTDGTIALGKFDEAQLLEMKGSQNALLLVDSDGTSQGIDSLVVDFKSSVQKVI
                     EHFVSHKAQHIAMLAGTEYTKKNHQRLKDPRIMAFQQVLAEKGYDEGPIIEADFSVES
                     GYQAVKQYLKTNPHVPDALFAANDALAIGALKAIQEKGLVVPEDISVMGFNDVSVAKY
                     VTPSLSTVKVYTEWMGELAVETILQLSNSKAPVSRKIIVETELIIRESTK"
BASE COUNT         1215 a          650 c          788 g          921 t
ORIGIN      
        1 tatataatca acaaacacaa ttttactaat caaaggagtg ttttttatga caattttagt
       61 actaggcgga gccggttata tcggttcgca cgctgtagac cagctagttc aaaaggggta
      121 tcaagtagct gtggtagata atttgctcac aggtcacaaa caagcagtac atccagatgc
      181 tcatttttat gaaggagaca tccgggataa agaatttcta cgttccgtat ttgaaaagga
      241 accgattgaa ggagtaatcc attttgctgc aagctcttta gtaggagaat ctgtcgaaaa
      301 accattgatg tatttcaaca acaacgtata cggtatgcaa atcttacttg aagtcatgca
      361 cgagttcaat gtgaataaaa tcgtgttctc ttctactgca gcaacttacg gagaaccaaa
      421 agagtctcca atcacagaag atacaccagc taatcctaaa aatccgtatg gtgaaagtaa
      481 attgatgatg gaaaaaatga tgaagtggtg tgaccaagca tatggcatgc gctatgtcgc
      541 tttgcgctac ttcaatgtag ccggagcaaa agcagatgca tctatcggag aagatcatac
      601 accagaaacg catcttgtcc caatcatttt acaagtagct cttggtcaaa gaaaagcatt
      661 ggctgtttat ggagacgatt atgatacacc agatggaaca tgtatcagag attatgttca
      721 agtagaagat ttgattgctg cacatatttt agctttggaa tacttgaaag aaggaaatga
      781 aagcaacttc ttcaatttag gcagcagcaa aggatactca gtcaaggaaa tgctagaagc
      841 agctagagaa gtgacaggaa aagaaattcc agcagaaata gcaccaagaa gagctggaga
      901 tcctagccga ttagtcgctt caagtgaaaa agcgagagag gttctgggtt ggaaacctga
      961 atacacagat atcaaagcaa ttatcaaaac agcatgggat tggcatgtca gccatccaaa
     1021 tggttatgaa gaatagggga atgagatatg tcaatcagtc aaacgatttg tgattttgcc
     1081 acacttgcga tcgaagctgg cggatggatg gaaatggacc gcttatattt acagaatcgt
     1141 cttttaggga tggtaggaga agatgctcta gaaagcattg aagtaagacc agttaaggaa
     1201 gaatctgtag acttattgga tcagcttgtc gctaaggcaa gagaaaatgg tgttctttca
     1261 gaattgccag cagaacaaga gatttttgaa gcgcaattaa tggattttct cacaccgcca
     1321 ccttcagtcg tcaatgcatt ctttgcacag cactattcga aaagcccaga ggaagcaact
     1381 gagtatttct ttaatttaag taagaaaaat gactacatca aaacaagagc catcgcaaaa
     1441 aacatcaaat ttccggcaga gtcggagtat ggtgaactgg aaatcacaat caatttatct
     1501 aagccagaaa aagatccaaa acaaatcgca gcagaacgcg aggcggtaaa agtagactat
     1561 cctacctgca tgctttgcat ggaaaacgaa ggatatagag gtcgcttgaa ctatccagcc
     1621 cgcaccaatc accggattat tcgaatgaat ctagacggtg aaagctgggg attccaatat
     1681 tctccttatg cttattacaa tgaacattcg atcattttat cagaacaaca ccgcccgatg
     1741 aaaatcacaa aggaaacatt tgctagactg cttcggatta ctgaagtctt gcctcattac
     1801 tttgttggtt cgaatgctga cttgccgatt gttggaggat cgattctttc tcatgatcat
     1861 tatcaggcag gacgtcatga atttcctatg gcaaaagcac cgatagaaaa acttatcaaa
     1921 ctagcagatt atccgcaagt catcgcagga gtcgtgaaat ggccgatgtc tgtgatccgt
     1981 ttgcaatcaa cagagaaaaa attgttgatt gaagcagcta actcagtatt ggaaaagtgg
     2041 aaagcatatt cggatgaaac agtatctatc agagcttttt cagcagatgg tacaccacac
     2101 catacgatta cgccaattgc acgaagaagg gacaattact ttgagttgga ccttgtcctt
     2161 cgagacaaca atgtttcaga agaacatcct gatgggattt ttcatccaca taaagatgtc
     2221 caacatatca aaaaggagaa cattgggttg atcgaagtaa tggggcttgc tgttttacca
     2281 cctcgtttga aaccagaact agtagaagta gagcatttct tattaggaga agaaaaccag
     2341 atggctgaat atcatcgtcc gtgggcagaa caattaaaaa aagaacatcc tgatttgacg
     2401 aaagatacgg tcaccgatgt cgtacaaaaa tctgtaggtc aggtttttgc acgtgtactg
     2461 gaagacgctg gagtattcaa gcgagatgaa aaaggacagg cagcgtttga acgatttact
     2521 gcaattttat aaacgtaaaa aaggaggaga gacaaatggc aacaattaaa gacattgcgc
     2581 agttggcagg agtttctcct gctactgttt caagagttct caactatgat cctgatcttt
     2641 ccgttggctt agccacaaag caaaaaattt ttgaagcagc agaacgctta aattatacga
     2701 aacatcgtaa aaatgcagca aatactaagc aaacgcttcg cctgatccag tggtacgacg
     2761 atacggaaga gcttgaagat ctttattatt tatctattcg tctaggaatc gaaaaaaagg
     2821 cagaagaagc aaacgttact ttattgaaag aaacgtggga ttccattagt gatcttccga
     2881 cagatggaac gatcgcacta ggaaaattcg acgaagctca gttattagaa atgaaaggat
     2941 cccaaaatgc cttgcttttg gtagattctg acggcacttc tcaaggaatc gactctctag
     3001 ttgtcgactt taagtcaagt gttcaaaaag tcattgaaca ttttgtctcc cataaagcgc
     3061 aacatatcgc tatgcttgct ggaacagaat atacgaagaa aaaccatcag cggctaaaag
     3121 atcccagaat tatggcattt caacaagttt tggcagaaaa aggctacgac gaaggtccaa
     3181 ttatcgaagc agatttttct gtcgagtcag gatatcaagc agtgaagcag tatttaaaaa
     3241 cgaatccgca tgttcctgat gcgctttttg cagctaatga tgcattagcg attggcgcac
     3301 tgaaagcgat tcaagaaaaa ggtttagtgg taccagaaga tatttctgtg atgggtttca
     3361 atgatgtcag tgtagcgaaa tatgtaacgc cctctctttc tactgtaaaa gtttataccg
     3421 aatggatggg agagctagca gtggagacga ttcttcaatt aagtaattcc aaagcaccgg
     3481 tttcaagaaa gattattgtg gaaacagagt tgattattcg agaatcaacg aaataaaaga
     3541 tggaaaagct gtggggaaat gaagtgatac cgca
//
LOCUS       DAZKLD010000192         3469 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.196, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000192 DAZKLD010000000
VERSION     DAZKLD010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3469)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3469)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3469
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.196"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(398..1372)
                     /gene="sgrA"
                     /locus_tag="ACG8KR_002763"
     CDS             complement(398..1372)
                     /gene="sgrA"
                     /locus_tag="ACG8KR_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored fibrinogen/nidogen-binding
                     adhesin SgrA"
                     /protein_id="HFQ0202071.1"
                     /translation="MKKTATVLFVILGLSIIAAMIGISTSANERANENLSFTVKTDRI
                     VYDMTQQVITIPVKPNKSVNASDVHAVLTYGWDGNGSSEKVIGEVYLKDVQWTAGIEY
                     TIMISAELSIDEIKSKDKVDLIVFYDGQMTITENLKPSSWTVVGPPSSTDSTSSESST
                     ENTSGESSTESTSSESSTENTSSESSTESTSSESSTGSTSSESSTESTSSESSTGSMS
                     SESSTGSTSSESSTGKVNNELDLVPIIPTKEQKRNAGSNLKSVLKQPDFVSRGIDHQE
                     KAKETDNRKSDLPETGSRTLNKLITWMGVLLILIVGASYFRSLFHRVK"
     gene            complement(1568..2407)
                     /locus_tag="ACG8KR_002764"
     CDS             complement(1568..2407)
                     /locus_tag="ACG8KR_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HFQ0202072.1"
                     /translation="MIRIIRLIGSVLIVLVGFVLGILVNNALADMFIGNSEWIGAIAG
                     TVGHLAVFLLALFLASKVEGKRIEDYGVSVRGKDWGYLAGGLVVGIGVFLLITSPLYL
                     TGAYRLDSGNANIVPLITSFILFIAVGASEELLFRGFFQHQMLRFGPLIAMIGSAALF
                     ALLHGLNPNMTFLAVFNIFLAGCFFSALIYSTGSLFTAIGAHITWNWTQGAILGIPVS
                     GTQEQGFFSTLIQSQNTIITGGNFGAEASISTTLFLLVLIVGLLLYAYKTKRMKKYTA
                     VYQ"
     gene            2592..>3469
                     /locus_tag="ACG8KR_002765"
     CDS             2592..>3469
                     /locus_tag="ACG8KR_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFQ0202073.1"
                     /translation="MGNFLKKQLLIMCLSACAILLFSGCSKSNETIATFKGGTLTVQD
                     FYDNPKVKSNNQTLLKKMIVFKAFDDVYGKEISSKEVTKEYNEQIKKLGPNYKEQLKA
                     VGQTEETYKKLFKQMLAFQYGLKANVKLTDKDLDTAWKEFYPEVSTQIILFSTKEEAD
                     KAKKEANEGENFSKLVQAYGKNKTLKETDGKMNFDSTNPAIPTEVKKAAFKLKNGEVS
                     DIIPVTDPTTYQQSYYLVKMVKKQDKGSNKDKYKSELEKIATEAKTLDTEFMDKTIRK
                     VMKKDNVTIKDPYVKN"
BASE COUNT         1161 a          717 c          508 g         1083 t
ORIGIN      
        1 cgatatcctt caccacatat aatttctcgt ctctttcata acaatagttc aaaaattgat
       61 atacttgcct ttcattgata tcaataaatt cttcaaacaa agtacatact cctttttatc
      121 tattatttag acttattccg acagctacat gaataacgta caaatagatt cgttactgat
      181 ttttcagaga tactaccatg cgcagtgtgc attgtcaatt attcttgctc ttattttcca
      241 tttgtgttcc tgttatttcc tgtttctcaa atatgttagt aaacaaaaat agatctggca
      301 aagctacaac ttcgccaggc aatacagcac tctcgaatga aatattcact caaaaatcta
      361 tcgttttatt caattttaga tctgttgctt ttattcttta tttcactcga tggaagagag
      421 aacgaaaata gcttgctcct acaatcaaaa tgagtaacac acccatccat gtaattagct
      481 tgttcaaggt tctactacca gtttctggta aatcgctttt tctattatct gtttctttcg
      541 ctttttcttg atgatcaatt ccacgagaaa caaaatcagg ctgttttagc acacttttta
      601 ggttgctccc tgcatttctc ttttgttcct tagttggtat gataggtacc aaatcaagtt
      661 cattatttac ttttcctgtg ctgctttcgc tgctcgtgct tcctgtgcta ctctcactac
      721 tcatgcttcc tgtgctactt tcactactcg tactctccgt actgctttcg ctgctcgtgc
      781 ttcctgtgct actttcacta ctcgtactct ccgtactgct ttcgctgctc gtgttttctg
      841 tgctactttc actgctcgta ctctctgtac tgctttcacc gctcgtgttt tctgtactgc
      901 tttcactact tgtgctatcc gtactgcttg gcggtccaac aactgtccaa ctgcttggtt
      961 tcaaattctc cgtgatcgtc atctgtccat cataaaaaac aatcaaatca actttatctt
     1021 tactcttgat ctcatcaatc gatagttctg cagaaatcat tattgtatac tcaataccag
     1081 cggtccattg tacgtctttt aaataaactt ccccaataac tttttctgaa cttccattac
     1141 catcccaacc atatgttaga acagcatgta catcactagc attcacgctt ttgtttggtt
     1201 tgactggaat agtaatgact tgttgtgtca tgtcataaac aatccgatca gttttcacag
     1261 taaatgaaag attctcattt gcccgttcat ttgcagaagt actgattcca atcattgctg
     1321 caattatact cagcccaaga ataacaaata atacggtcgc tgttttcttc atttctatca
     1381 ctctccatat aattaccttc tatttgataa aggaattata taaaatgaaa atgatgagaa
     1441 cacgacagtc agttgtgaaa atctaacaaa agatgtcgtg tattcgcttg aatttgttta
     1501 ctttttcgtt taatcaatga cagacttaaa aaagcaagac cgatatcagc cttgctttct
     1561 tcatctttta ttgataaaca gcagtgtatt tcttcatccg ctttgtttta tatgcataga
     1621 gtaacaaacc aacaatcaag actaacaaga ataatgtagt tgatatagat gcttcagccc
     1681 caaagtttcc cccagtaatt attgtatttt gcgattggat cagagtggag aaaaaacctt
     1741 gttcttgcgt tccgctgact ggtataccca aaatagctcc ttgtgtccaa ttccaagtaa
     1801 tatgagcacc aatagccgta aataaacttc ctgtactgta gataagtgcg ctgaaaaagc
     1861 aacctgctaa aaagatattg aaaactgcca gaaatgtcat gttaggattc aatccgtgca
     1921 aaagagcaaa taatgcagca gatccaatca ttgctattaa cggaccaaat ctaagcatct
     1981 gatgctgaaa aaagccacgg aataaaagct cttctgatgc ccccactgca ataaataaaa
     2041 taaaactcgt tatcagcggc acaatgtttg cgtttcctga atctaaccga tacgcccccg
     2101 tcaaatataa tggactagta ataagcagaa acactccaat tccaacaacg agacctcccg
     2161 ctaaataccc ccagtctttt cctcgaacag aaacaccata atcttctatt ctttttcctt
     2221 cgaccttact agcaagaaat aaagctagta aaaaaacagc taaatgtccg accgtaccgg
     2281 caattgctcc aatccattca ctatttccaa taaacatatc cgcaagtgca ttattgacaa
     2341 gaattcctaa aacaaaacca actaagacta ttaaaacaga tccaatcaac cgaataatcc
     2401 tgatcatctt tcatccctcc tttatattaa gtttaacaac ccttttcttt cttgtctcgt
     2461 gaaaactatg taaatgttct ccatcataga gagaatagat ttttacgaac taagaatatc
     2521 tacagcaacc ttacgaaaat gaaagactgt ctccctaatt cttctgttag actgttttta
     2581 ataaggaaag gatgggaaat tttttgaaaa aacaactact aattatgtgc ctttcagcat
     2641 gtgcaatttt actattctca ggttgtagca agtccaatga aacgattgca acgtttaaag
     2701 gtggaacact tacagttcag gatttttatg ataacccaaa agttaaatca aataatcaaa
     2761 cactattaaa aaaaatgatt gtcttcaaag catttgatga tgtatacgga aaagagattt
     2821 cttcaaagga agtaacaaaa gaatataatg aacaaatcaa aaaactcggt ccaaactaca
     2881 aggaacaatt aaaagcagtt ggccaaacag aagagacata taagaaactc tttaaacaga
     2941 tgctggcttt ccaatacggt ttgaaagcca atgtaaaatt aacagataaa gatctagaca
     3001 ctgcttggaa agaattctat ccagaagtta gtacacagat cattctattt tcgactaaag
     3061 aagaagcaga taaagcaaaa aaagaagcaa atgaaggaga aaatttctcc aagcttgttc
     3121 aagcttatgg aaaaaataaa acattaaagg aaactgatgg aaaaatgaat tttgattcaa
     3181 ccaatccagc gattcctact gaagtaaaaa aagcagcatt taaactaaaa aacggggaag
     3241 tttctgatat cattcctgta acagatccaa cgacttatca gcaaagctat tatctagtaa
     3301 aaatggtaaa aaaacaggat aaaggttcta ataaagacaa atacaaatct gagctagaaa
     3361 aaattgctac cgaagcaaaa actcttgata cggaattcat ggataaaaca atccgtaaag
     3421 tgatgaaaaa agacaatgta actattaaag atccttatgt aaaaaatat
//
LOCUS       DAZKLD010000193         3419 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.197, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000193 DAZKLD010000000
VERSION     DAZKLD010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3419
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.197"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..418
                     /locus_tag="ACG8KR_002766"
     CDS             <1..418
                     /locus_tag="ACG8KR_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFQ0202074.1"
                     /translation="TTWGVIIPQFADAMGIFLLTQTMREIPVSLLDVAALDNIKQTTV
                     MSKIVFPLTRHAITSTGIWFFITSWNEYVWPVLILRTVDNYTLPLAMQTYISSEGGTN
                     FTVAMAISLITMFVPLVLYAIFQKYIIGTFVSAGIK"
     gene            434..1558
                     /locus_tag="ACG8KR_002767"
     CDS             434..1558
                     /locus_tag="ACG8KR_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0202075.1"
                     /translation="MGETLELRNIGKKYGEHTILKEIDLAVAAGERIVLLGPSGSGKS
                     TLLRMIAGLEKITSGELFLGTQLANKLPCGEREIAMVFQNYALYPHMTVKENIIFALR
                     ANKIKTADIQHRLEEALKMLGLTPYQDRLPKDLSGGQRQRVALARAVVKRSNYFLLDE
                     PLSNLDVRLRLDARKELVKIHQRYKQTFVYVTHDQIEAMTLADRIVLLNEGRIQMIDT
                     PKNVYTKPKNTFTANFIGSPGMNIMPVTYDQGTLKVASQFLTLNPAWQKQLLQVSGSL
                     YLGIRPEHVTFSKETSDLHGQVKYRELLGQSYAYTVKVDGIEMIVLNEKPTWQIGDAV
                     NLRIEPERMHFFDYQTQENLGYPDYFIEERGNSHALFASL"
     gene            1536..2339
                     /locus_tag="ACG8KR_002768"
     CDS             1536..2339
                     /locus_tag="ACG8KR_002768"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFQ0202076.1"
                     /translation="MRFLHLSDIHFRENYQTNLFTAELFPTIKNPQLALEKLLQELSL
                     TVYDFILLTGDLVHEGGISDYQVLRKMLDQYFPTTPYYFCRGNHDRRSVFFEGMSVES
                     NETGEYIHCTNQNGLRIISLDSAQDTQHEGKISIGQMIQLQEWLGTPAEKGTILLVHH
                     PLSWEDPSIVTEVPEGFAELIAESDILGIFVGHIHQGSSAEFAGKKQFMAESLSFGVD
                     EYPDYSLFTNRTGFTSGSLIDGKIFTYQHYLTPHQIEIGRMKKPITGNH"
     gene            2799..3062
                     /locus_tag="ACG8KR_002769"
     CDS             2799..3062
                     /locus_tag="ACG8KR_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HFQ0202077.1"
                     /translation="MAKLQISMDEKLKNEAESIIDELGMSPKTAVTVFYKQIVEQGKI
                     PFSIELSEKSKRIHNIQRLAEKLPVQKLDTDEKIEEWFNEDEY"
     gene            3046..3381
                     /locus_tag="ACG8KR_002770"
     CDS             3046..3381
                     /locus_tag="ACG8KR_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFQ0202078.1"
                     /translation="MKMNTNDIATVYVAFTAAEGGKRRPVYILKNGLDTISFLSITSK
                     YHTKSDKIKLKYVEIRDWKQAGLKKESWIDVGTLNELPKNNKKVKWKKIGELSPSDLQ
                     RLEERMEEL"
BASE COUNT         1126 a          546 c          694 g         1053 t
ORIGIN      
        1 tactacttgg ggtgtgataa taccacaatt tgctgatgcg atgggcattt ttttattgac
       61 gcaaacaatg cgggaaattc cagtgagcct tctagatgtg gcggcattgg ataacatcaa
      121 acaaacaacc gtcatgagta aaattgtttt tcctcttact agacatgcga ttacttctac
      181 cggtatttgg ttttttatta cctcttggaa tgagtatgtt tggccggtat taattttacg
      241 gacagtagat aattatactt tgccgttggc gatgcagacc tatattagtt ctgaaggtgg
      301 gacaaatttt accgtagcaa tggcgattag tttaattaca atgtttgtac cgttagtctt
      361 gtatgcaatt tttcaaaaat atattatcgg gacttttgtt tctgcaggta ttaaataaat
      421 tggaggacaa atcatgggag aaacgcttga attaagaaat atcggaaaaa aatatggaga
      481 acataccatt ttaaaagaaa ttgatttagc tgtggccgct ggagaacgaa ttgtgttact
      541 gggaccatct ggttcaggaa aatctacttt gctgagaatg attgctggtt tggaaaaaat
      601 tacgagtgga gagctttttt taggtacaca actagcaaat aagctacctt gtggagaacg
      661 agagatcgca atggtttttc aaaattatgc tttatatcct cacatgactg taaaggagaa
      721 tattattttt gcattaaggg cgaataaaat taagacagct gacattcagc accgtttgga
      781 agaagctttg aagatgttag gtttaacgcc ataccaagat cgacttccta aagatttgtc
      841 aggagggcaa cggcagcgtg tagcattggc tcgtgcggtg gttaaacgga gtaattattt
      901 tttattagat gaaccgttat caaatttgga tgtgcgttta cgattggatg cacgaaaaga
      961 attggtaaaa attcatcaac gttataaaca aacttttgtc tacgtcactc atgatcaaat
     1021 tgaagcgatg acgttagctg accggattgt tttattgaat gaaggtagaa ttcagatgat
     1081 tgatacgccg aaaaatgttt atacgaaacc taaaaatact tttactgcaa attttattgg
     1141 ttcgccaggc atgaatatta tgccagttac ttatgatcaa ggaacattaa aggtagccag
     1201 tcaattttta acattgaatc cagcttggca aaaacaactt cttcaggtta gtggctcatt
     1261 gtatttaggc ataagaccag aacacgtaac cttttcaaaa gaaacaagcg atttacacgg
     1321 tcaagtgaag tatcgtgagc tattaggtca aagttatgcg tacaccgtta aagtcgatgg
     1381 tatagaaatg attgtcctta atgaaaaacc tacttggcag attggtgatg cagtcaatct
     1441 ccgcatagaa cctgaaagaa tgcatttttt tgattatcag acgcaagaaa atttaggata
     1501 tcctgattat tttattgaag aaagagggaa tagccatgcg ctttttgcat ctctctgata
     1561 tccattttag agaaaactat caaactaact tgtttactgc tgaactattt ccaacaatta
     1621 aaaatccaca gttggcattg gaaaaattgt tacaagaact ctcgttaaca gtgtacgatt
     1681 ttattctatt aacaggtgat ttagtacatg aaggggggat ttctgattat caagtcttac
     1741 ggaaaatgtt ggatcaatat ttcccaacca caccttacta tttttgtcgt gggaatcatg
     1801 atcgtcgcag tgttttcttt gaaggaatga gtgttgaaag caatgaaacg ggagagtata
     1861 tccattgtac gaatcaaaat gggttacgaa ttatttcctt ggattctgct caagataccc
     1921 agcacgaagg aaaaatatcg ataggacaga tgattcaatt acaagagtgg ttgggtacac
     1981 ctgctgaaaa gggaacgatt ttattggttc atcatccatt gtcgtgggaa gatccatcta
     2041 ttgtaactga agttccggaa ggttttgctg aattgattgc cgaaagtgat attttaggta
     2101 tttttgtagg acatatccat caaggtagca gtgctgaatt tgctggtaaa aaacagttta
     2161 tggctgaatc tttatcattt ggtgtagatg aatacccaga ctattctctt tttaccaata
     2221 gaacaggatt cactagtggc agtttaattg acggaaaaat ttttacttat cagcattatt
     2281 taactccaca tcaaatagaa atagggcgca tgaaaaaacc aattactggt aatcattaaa
     2341 aattattgat taaaaataaa gacttcattc actttttagc cgtggtgctc aatgtctggt
     2401 tccacttgaa gaacttggaa aatcgctatc aataaaccac tatccctcca attacaaaaa
     2461 gggtggagaa agttttccaa gaaccatatc ggtcttcgtg gttgtgccat tcgtttttta
     2521 ccagagcata ccacatcttt gtggagtttt cagacacgtc ctagttaatt tggttctttt
     2581 aggagtcttt tcctgttctg tgtgtcgttg agtgttcgct tcaattatcg ctgtggcgga
     2641 ttttgaaact tttctatcta tcaaggccat ttagcactat atcctttcct tatatttaga
     2701 taaatacact gcatttcaag gagttcaaaa gtagttgtat taattccatt tgaactcttt
     2761 ccgtgatata atgtgtttgt cagaaagagg tggagaaaat ggctaagtta caaatttcta
     2821 tggacgaaaa attgaaaaat gaagcagaaa gcattattga tgaactaggc atgagcccca
     2881 aaacagccgt caccgttttt tataagcaaa ttgttgagca aggaaaaatc ccattttcta
     2941 ttgaattgtc ggaaaaaagt aaacgaatcc ataatattca aagattagcg gaaaaattgc
     3001 ctgtccaaaa actagacact gacgaaaaaa ttgaagaatg gttcaatgaa gatgaatact
     3061 aatgatatag caaccgtcta cgttgctttc acagctgcag agggcggtaa acgccggcct
     3121 gtctatattc taaaaaatgg actggacaca atttcgtttc tgtcaattac ttcaaaatat
     3181 catactaaat ccgataaaat aaagctgaaa tacgttgaaa taagggattg gaaacaggca
     3241 ggactaaaaa aagaatcttg gattgacgta ggaactctaa acgaattacc caaaaacaac
     3301 aaaaaggtca aatggaaaaa aattggggaa ttaagtccaa gcgatttaca gcgactggaa
     3361 gaacgcatgg aagaattata ataatactaa aaagaagggc tctttgtcaa taaggactg
//
LOCUS       DAZKLD010000194         3384 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.198, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000194 DAZKLD010000000
VERSION     DAZKLD010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3384)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3384
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.198"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(561..1601)
                     /locus_tag="ACG8KR_002771"
     CDS             complement(561..1601)
                     /locus_tag="ACG8KR_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HFQ0202079.1"
                     /translation="MNDFNYYKSKEIYREKYYQMPKVFFTNEKYMDLSNDAKIAYMLL
                     KDRFDYSVKNNWVDSDDNIFFIFTVEELMKLLQCREGKVSKIKKELEKAGLLKQKRGR
                     VNKRDGKIESMPNLLYLGKPEVTNQDVFKIMEEEDNTDTTVIAKIANTAKPREINDTT
                     VIAKIANTAKSSHIKDSTVIAKIADNLFYSNSIDTNRHIIDTETDQLQNQVLLDNFVD
                     IMKEDSINTFVPENVLNLIKTFSSTYEDAQKTVQTIHNAKKKAEELEQTVVVYEELES
                     YGIDADKGLYMTLLKAYQKQKTEKVNNLQNLIFVYVKNWFVEKAIAAKLANEQRETFN
                     ELPTVSTENWLE"
     gene            complement(1950..2276)
                     /locus_tag="ACG8KR_002772"
     CDS             complement(1950..2276)
                     /locus_tag="ACG8KR_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0202080.1"
                     /translation="MAEDNRSQAAVSRLLGNKNKKNFKSKEVEEIELTKKNISKVIKD
                     QGSSVRTADSIRINPILMSALKYWTTIAESDKSKPDVIEDALLKSIPEEYLIEGYKLA
                     KKQNKI"
     gene            complement(2263..3051)
                     /locus_tag="ACG8KR_002773"
     CDS             complement(2263..3051)
                     /locus_tag="ACG8KR_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFQ0202081.1"
                     /translation="MARILSVANQKGGVSKTTTSVNLAACLKFVYGKNVLLVDIDPQG
                     NATDNVGFDIDGTNQPTIYEVLKGEADITDAILDYKGIDVLPADIALSSAEREFTQVG
                     SEHRLKRVLQPIKDNYDYIIIDCPPSLGMLTVNAFTVSDEIIIPVEAAYFSLKGLIKL
                     SETIETVKEYTNDKLEVRGVLFTKYNKRYNISKEMTSAATEISRVIGAKIFKTYIRRT
                     ITVDEAQAAGSDLINFNKPSTAEDDYKAFTEEYLKEVGDFDGRR"
BASE COUNT         1029 a          565 c          466 g         1324 t
ORIGIN      
        1 tccgttgcgg gtaataccgt aacactgaag ggcacgcatt aaggcgtcat cttaatgctt
       61 tcctgtagga cactgacgga gattaccagt ctcccgagcc ttacaggggc ctttcttttt
      121 tttgtatttt caattatcac aatttatcgt ccaaaagtgc agaacgaaaa tcaattcatc
      181 acaagcatat cagacaatta aatttaaaac aactaaaaat atacaaaaaa gtgtaattga
      241 atattagcag cactgcctga aaagaagtaa tatcaggctt ttcagcttgg ggaaataagt
      301 gaattacctc tcttcatgaa ttattataac tcaaaaaagc acatatgcaa acactagttc
      361 cctatttttt tgaatgaaaa ataactaaaa tatctaacga ttatgacttt aaaacctacg
      421 tttgtgattg ttttcataaa cttttaatct aaaaactccg gaataatagc tatttatttg
      481 taacatttgt aataaaatta aaatcataaa aaagttctaa tttaaaattg atcattttta
      541 tagtgatcgc tttttatcaa ctactctagc caattttccg tactaactgt tggtaattcg
      601 ttaaatgttt ctctttgttc gtttgcaagc tttgccgcaa tagctttttc gacaaaccaa
      661 ttttttacat aaacgaaaat tagattctgc aaattgttta ctttttcagt tttttgtttt
      721 tgataagctt tcagcaaagt catatataat cctttgtcag catcaattcc ataagattcc
      781 agttcttcat aaacaacaac tgtttgctct aattcttcag cttttttctt tgcattatga
      841 atagtttgga cggttttttg agcgtcttcg taagtactag aaaatgtttt tatcaagttt
      901 aatacatttt ctggtacaaa tgtatttata ctgtcctctt tcatgatgtc gacgaagtta
      961 tctagtaaaa cttgattttg taactgatca gtttcagtgt ctattatgtg tctattagtg
     1021 tctatagaat tagaatagaa tagattgtct gcgattttcg caattacggt tgagtccttg
     1081 atatgactgg attttgccgt atttgcgatt ttcgcaatta cggtggtatc attgatttct
     1141 cgcggttttg ccgtatttgc gattttcgca attacggtgg tatcagtatt gtcttcctct
     1201 tccatgattt taaagacgtc ttgattcgtt acttctggct tgcctaagta caataaatta
     1261 ggcatggatt caatcttacc gtcacgttta tttactcttc ctctcttctg tttcagaaga
     1321 cctgcttttt ctaattcctt tttgatttta gatactttac cttcgcggca ttgtaacaat
     1381 ttcattaatt cttcaactgt gaatatgaaa aatatattat catcagaatc tacccagtta
     1441 tttttgactg aataatcaaa tcgatcttta agtagcatat aagcaatttt tgcatcattt
     1501 gatagatcca tgtatttttc gttagtaaaa aataccttag gcatttgata atatttttca
     1561 cgataaattt cttttgattt gtaataatta aaatcattca tagccaatcc tctttctgta
     1621 ggatagcgtt ttgttcaaaa ctatgttaca atgaattaaa caaatatatc tatccactat
     1681 atatttttct actgctgata agtcgccaaa ctttgttaaa gcagtagtct ttttttatct
     1741 ttttttagtt taccttaaaa aataaattaa agcaaaccgc tctatttcaa ggtttaagct
     1801 catagtaact atatcacgat attacaataa tattatatca cgatatcgta atattacaat
     1861 atgaagataa gcgacttatc agctttgata ctactgattt ttataaaaaa aatatcacga
     1921 tattatgata tcgtgatatt gaatgtaaat taaattttat tttgtttctt tgctagttta
     1981 tatccttcaa tcaagtattc ttcaggtatg gactttaata gcgcatcttc tataacgtct
     2041 ggtttacttt tgtctgattc agcaatagtc gtccaatatt tgagtgctga cattagaatt
     2101 ggatttatac gaatgctatc tgctgttctt acactacttc cttgatcttt tatgacttta
     2161 cttatatttt tcttggttaa ttcgatttct tctacttctt ttgatttaaa attcttttta
     2221 tttttatttc ctagtaatct acttactgca gcttgactgc gattatcttc tgccatcaaa
     2281 atcaccaact tcttttaaat attcttctgt aaatgcttta tagtcatctt cagctgttga
     2341 gggtttatta aaattgatta gatctgatcc agcagcttgc gcttcgtcaa cggttattgt
     2401 cctacgaata taagttttga aaatttttgc accaatgact ctagaaattt cggttgctgc
     2461 agacgtcatt tctttagaaa tgttataacg cttattatac ttagtaaata gaactcctcg
     2521 tacttcaagc ttgtcgttcg tatattcttt taccgtttca atagtttcac ttaactttat
     2581 tagtcctttt aatgagaaat acgcagcttc aactggaatg ataatttcat cagaaacagt
     2641 aaatgcgttg actgtaagca ttcctaaact tggtggacaa tcaattataa tataatcata
     2701 gttgtccttg attggttgaa gtactctttt aagcctgtgt tcacttccta cttgagtaaa
     2761 ttccctctct gcagaagaaa gggctatgtc ggctggcaaa acatcaattc ctttatagtc
     2821 cagaatagca tctgtaatgt cagcctcccc ttttaatact tcataaattg taggttggtt
     2881 tgttccatct atatcgaaac ctacattatc tgtagcattt ccctgaggat caatgtccac
     2941 taaaagtaca tttttaccgt aaacaaattt caaacatgct gctaaattaa cgctagtcgt
     3001 agttttagaa actccacctt tttgattggc tactgacaat attcttgcca ttaaaatatc
     3061 ccccattcat cttatattat aatataatta tatcataata ttgtgatatt gcaatatcgt
     3121 atatgcatta tattgtaata ttgtgtgatt attttgtttt aaagatatat atcggttcgg
     3181 atataaagat tattcatatg ttattcgaaa ttaaaataat cactttttat tactttaaaa
     3241 ttttttatta atgataagaa atatgttttg cgagcagcaa ctagaataat ttaaacatta
     3301 ctaaactgtc tatattgtta ctaataattc ccttcttata gtgcattttc ttagaaaaaa
     3361 acaatattac gatatcatga tatt
//
LOCUS       DAZKLD010000195         3335 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.199, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000195 DAZKLD010000000
VERSION     DAZKLD010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3335
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.199"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(368..1486)
                     /locus_tag="ACG8KR_002774"
     CDS             complement(368..1486)
                     /locus_tag="ACG8KR_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0202082.1"
                     /translation="MAKKDKELLTQLTSEGAKGPFVTIMLNTHVAHQDVEKDQIKFKN
                     FAKEAKKRFEKKYSEHDWSPFQEKIENLLGDPSFWRSATTSVAVIITADQTYIHRLSI
                     PVDDQYYVSDLPYLLAIIKNGQFNYSYYLLGLNRDSMKLYEVSNKQVKEVSLPEGAPV
                     DVVTALGSELTGGGLNFSSNGGGGARDGVTFHGVNPKDEEVEIDWVNYYQAVDTYLKD
                     SFENPEQWPIYLFALPENQTMFKKIAKNPYYCADVAISASPAQLSFRDIEEGAKKLSS
                     ELAAQESKEYNKLLDKKFIDQLVDIVPAAKEGKVSHLFIATSNLADGFGEDPETEYDR
                     RQVLNALADDVIINGGKVFILDQKDAPDEKSLTAILRY"
     gene            1719..1841
                     /locus_tag="ACG8KR_002775"
     CDS             1719..1841
                     /locus_tag="ACG8KR_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0202083.1"
                     /translation="MHLIMIFSLLSIISLFAFSASRLLDAETQPIPVKVRKNDR"
     gene            1957..3264
                     /locus_tag="ACG8KR_002776"
     CDS             1957..3264
                     /locus_tag="ACG8KR_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HFQ0202084.1"
                     /translation="MSIKKTRLVLLLASLILCLASFLPAVAVQAEDTFKVNAKAAFAV
                     DAQSGKILYDQDGEKTMGIASITKIIGLYIVLDQVKEGKLSWGDKVSISDYAENLSIT
                     PDLSNVPLHKENTYTVKELFDSAIIQSANASMVALAEKISGSEAKFTERMKEQLKDWG
                     IKDATIVNASGLNNSYLGENRPEGTGENDENQMSAQDVAIVARHLILDFPEILDVSST
                     TTQMFGENTQSPVEMVNWNWMLPGFVNYKEGVDGLKTGTTELAGACFVGTITKNDQRI
                     ITVVLNADGHAENPSARFNETDRLMDYCYDNWSEKELGKANASIPSLKTIDVKDGKES
                     TVPVVLKSPVKVWVRNDMDTGKLTITPTLDKKQVTDNEIQAPMDKGTKIGTATITLAD
                     DKLGYLEDNNAPSTEIITDKTVEKANIFVIGWRRVAEFFGNLF"
BASE COUNT         1045 a          688 c          597 g         1005 t
ORIGIN      
        1 tttttaaacg gtgttccaga agtagcttct tcggaaataa gccgaaattt cacaaaaatt
       61 tgagaaataa tttttgtcaa tctcttctta tttcttgaag ctgaccactt ctgtcacaac
      121 ctcttcctta aacggtgttc aaaagcttgc atctgcggta ataagcccac caaacaaaaa
      181 atatgaagag ctatttttgc ttgctgttct tattacttga tgcagaacag ctttttcaca
      241 acctctcata cacggtgttt aatcagctga ccttcggtat tagctcaaaa aacggaaaaa
      301 tatgtgaaac tatttttccg tttgtttctc taatactcag gtcaaaacac tgatttcaca
      361 acctctttta ataacgaaga atcgctgtca gactcttttc atctggcgca tctttttgat
      421 ccaaaataaa cactttcccg ccattaatga tcacatcatc tgctaatgca ttcaacactt
      481 gacgccgatc atattctgtt tcaggatctt caccgaaacc atcagctaag tttgacgtag
      541 cgataaataa atgtgacact tttccttctt tcgcagctgg aacgatatca actaactgat
      601 cgatgaattt cttatctaag agtttattgt attcttttga ttcttgagct gcgagttcac
      661 ttgacagctt cttcgcccct tcttcgatat ctctaaagga tagctgcgca ggagaagcag
      721 aaatcgcaac gtctgcacaa taatagggat ttttagcaat ttttttgaac atcgtctgat
      781 tttctggtaa tgcaaataaa taaatcggcc attgttcagg attttcgaaa gaatccttca
      841 agtacgtatc aacagcttgg taatagttga cccaatcgat ctctacttct tcatcttttg
      901 gatttacgcc atggaaagtt actccatctc gggctccgcc tcctccatta gaagagaagt
      961 tcaaaccgcc acctgtcagt tcagatccca atgcagtaac gacatcaacc ggtgcccctt
     1021 ctggtagaga tacttctttt acttgtttgt tgctgacctc atacaatttc atggagtcgc
     1081 ggttcaatcc caataaataa taagaatagt tgaactgccc atttttgata atagccagta
     1141 aataaggaag atcacttaca tagtattgat cgtctaccgg gatactcaaa cgatggatat
     1201 aggtctggtc agcagttata atgaccgcta cactcgtagt tgcactacgc caaaacgaag
     1261 gatctcctaa caaattttca attttttctt ggaacgggga ccagtcatgt tcggaatatt
     1321 ttttctcaaa acgtttcttt gcttctttcg caaaattttt gaatttgatc tgatcttttt
     1381 ccacatcttg atgggcgaca tgcgtattca gcatgatcgt gacgaatggt cctttagcgc
     1441 cttctgaagt caattgtgtc aataattctt tatccttttt tgccataaac gtacccctcc
     1501 taaaaaaatc attcctttgt ttctatctaa agtctaccac aacactattt ttccatacaa
     1561 taaaacgtat ctaaaaaaat aaaacggttt ctacggtttt aaatttagct atttttttaa
     1621 gttatcttca taatttcctc aattatttag ccccagtctt caactattct tcatgaaatg
     1681 ctgataaact aagaaaaaga ataaaagagg tggatcttat gcatttgatt atgattttca
     1741 gtttattaag tattatcagt ttatttgctt tcagcgcttc ccgcttatta gatgcggaaa
     1801 cacagcctat tcctgtaaaa gtacgtaaaa acgaccgcta aacttccata acgcgcgttc
     1861 ttatcctaaa gttttttcag aaacattgaa actctctccc ctctctgata aaattttaat
     1921 ttgtagcgaa acaaattaat tacaaggaga tcggatatgt ccataaaaaa aacacgtcta
     1981 gtcctattat tggctagtct cattctttgc cttgcttctt ttctaccagc tgttgctgtg
     2041 caagctgaag atacgttcaa agtaaatgca aaagcagctt ttgctgtaga tgcgcaaagc
     2101 ggtaagattc tttatgatca agatggagaa aaaaccatgg ggatcgcttc aatcacaaaa
     2161 atcatcggtc tttacatcgt ccttgatcaa gtaaaagaag gaaaactgtc atggggtgat
     2221 aaagtttcga tttcagatta tgcagaaaat ttgagtatca ccccagattt atcgaatgtt
     2281 cctttgcata aagaaaacac ctacactgtc aaagaattat tcgactctgc aatcatccag
     2341 tcagccaatg cttcaatggt ggccttggca gaaaaaatct ctggcagcga agctaaattc
     2401 actgaacgaa tgaaagaaca attgaaagat tggggaatca aagatgcaac aatcgttaat
     2461 gcatcaggat tgaacaactc ttatcttggc gaaaaccgac cagaaggcac tggagaaaac
     2521 gacgaaaatc aaatgtctgc tcaagatgtg gccatcgttg cccgccattt gatcttggat
     2581 ttcccagaaa tactggatgt atcaagtact acgacccaaa tgtttggcga aaatacgcaa
     2641 tcaccagtgg aaatggtcaa ctggaactgg atgctcccag gattcgttaa ttacaaggaa
     2701 ggcgtggacg gattaaaaac tggtacgact gagcttgcag gtgcttgttt cgttggtacg
     2761 atcactaaaa acgatcaacg gatcatcact gtcgtattga atgcggacgg ccatgcggaa
     2821 aacccgagtg cccgcttcaa tgaaacagac cgtttgatgg actactgcta tgacaattgg
     2881 tcagaaaaag agctcggaaa agctaacgca agtattccta gtctaaaaac cattgatgta
     2941 aaagacggaa aagaatcaac agttcctgtt gtgcttaaaa gccctgtaaa agtatgggta
     3001 cgaaacgata tggatactgg caaattaacg atcacgccaa cacttgacaa gaagcaagtc
     3061 actgacaatg agatccaagc acctatggat aaaggtacaa aaatcggtac cgctacgatc
     3121 accttggcag acgataaatt aggctattta gaagacaaca atgcaccatc aacagagatc
     3181 attacggata aaacagtgga aaaagcgaat atattcgtga ttggctggcg acgcgtcgca
     3241 gaatttttcg gcaacttgtt ttaattgaac aataagaaaa acatctttcc atcggaaaga
     3301 tgtttttttg ctttttctag tttaaataac agctt
//
LOCUS       DAZKLD010000196         3306 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.200, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000196 DAZKLD010000000
VERSION     DAZKLD010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3306)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3306)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3306
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.200"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..2177)
                     /locus_tag="ACG8KR_002777"
     CDS             complement(<1..2177)
                     /locus_tag="ACG8KR_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prealbumin-like fold domain-containing protein"
                     /protein_id="HFQ0202085.1"
                     /translation="MRNKFHKLLSMFLVMLIVINQFGNITVLAESLAEQPERSLTIEV
                     DQEEAALGEQFQLRVRGTPDLLNALVIDKSEDLIEGEVKQLNDYERLITYSSQHSGKY
                     ELTARSGDIQSNTVSIEISVPEESTSESFNVPEESTSESFNIPEESLSESFNEARSSE
                     PSITQESQIERQEELLNYDIGISAERPTLPSRHTQTLKGYIQGGAGDILEEYGEMVVR
                     VPRASIGLIEFIRVEVADDSPLKPLAFYQEGTDVVFPFTFDYEGLNEAAQSYTFSITF
                     EVAFFQSGGTEPNNDFQVSLTYAERAANTEFSITPIPSSRPIFSKLYYGSREIDSQGN
                     LGNLLIDGQDVTNSNKFILQVNYEGSRKDGVVVTDVLPQGTTLAPNPTTWSSTGNREP
                     ISGGVRIAEVTNPGEANVRTHYVSEEFVDKVSFNKETNTLVVDFGNLAEGQSYFIEYA
                     LEVTDVDLFNEGRQEDGFSRNRASMTYNNQVMSRDYRLRIPESNLPAISLRKSVDKNV
                     INLNENELTYTLHVSMFSGTRNTLVIEDVLEEGMEFVSFLSDIPSFIDAEVNNGIVRF
                     SANNTIGGPTSFTLSFKVNVEAYAVGDQILNFATLVNSDNRLNTSTVRTRKIDGRIKV
                     IKTDENGQRLAGAKFEILSSDSQVIQEGITAENGEFLSEPLPVGSYQVRETEAPEGYL
                     LDATVHDVFINEYSIEEVTLTIENILDTGGVELIKHAEGSGEVL"
     gene            complement(2849..3117)
                     /locus_tag="ACG8KR_002778"
                     /pseudo
     CDS             complement(2849..3117)
                     /locus_tag="ACG8KR_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720767.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS607 family transposase"
BASE COUNT          915 a          733 c          531 g         1127 t
ORIGIN      
        1 aggacttcac cactgccttc agcatgttta attagttcaa caccacccgt atcaaggatg
       61 ttttcaatgg ttaacgtcac ttcttctatg gagtattcat ttataaaaac atcatgtacc
      121 gtcgcatcta aaagataccc ttccggtgct tctgtttctc ggacttgata gctacctaca
      181 ggaagcggtt cacttaaaaa ttctccgttt tctgccgtta taccttcttg tatcacttgt
      241 gaatcgcttg ataaaatttc aaattttgct cctgccagcc tttggccatt ctcatcagtt
      301 ttaattactt taatacgccc atcgattttc cttgttctta cagtactcgt attcaatcga
      361 ttgtcactat tcacaagcgt tgcaaagttt aaaatttgat cgccgaccgc atacgcctct
      421 acattgacct taaaagagag ggtaaaactt gtcggaccac caatcgtatt attcgcactg
      481 aaacgaacta ttccattatt gacttcggca tcaatgaaag agggaatatc tgatagaaat
      541 gacacaaatt ccatgccttc ttccaaaaca tcttcaataa caagcgtatt tcttgttcca
      601 gaaaacatcg agacatgcag agtatacgtt agttcatttt cattgagatt gataacattt
      661 ttgtccacac tttttcttaa agagattgcc ggaagattac tttcagggat tctcaagcgg
      721 tagtctcggc tcattacctg attattgtag gtcatactcg ctcgattccg agaaaaccca
      781 tcctcttgtc ttccttcatt gaacaagtcg acatcggtca cttcaagagc atattcaata
      841 aaatacgatt gaccctctgc tagattacca aagtccacaa caagtgtatt cgtttcctta
      901 ttaaaagata ctttgtccac aaattcctcg gaaacataat gagttctaac gtttgcttca
      961 cctggattcg ttacttctgc gatccgaacc ccacctgaaa taggttctct atttccagtg
     1021 gaactccatg tagtcggatt cggtgccaaa gttgttcctt gcggtagtac atcggtaacc
     1081 actacaccgt cttttcgtga tccttcataa tttacctgca aaataaattt attactgttg
     1141 gtcacgtctt gcccgtcaat cagcagattc ccaagatttc cttggctgtc gatttctcta
     1201 gagccatagt ataatttcga aaaaatgggt cttgatgaag gaataggcgt aattgaaaat
     1261 tccgtgtttg ctgctcgctc agcataagta agactcactt gaaagtcatt atttggctca
     1321 gtaccgcctg attgaaagaa agcaacttca aacgtgatac tgaatgtata cgactgagcg
     1381 gcttcattta atccttcata atcaaaggta aatggaaaaa caacgtctgt gccttcttgg
     1441 taaaatgcca gtggctttaa aggtgaatca tcagcgacct ccactctaat gaactcaatt
     1501 aagccaatcg atgctctagg aactcgtacg accatctccc catattcttc aagaatatcg
     1561 ccagcgcctc cttgaatgta tccctttaac gtttgtgtat gcctgctcgg caaagtgggg
     1621 cgctcagcag agatgccgat gtcataattc aagagctcct cttgtctttc aatctgagat
     1681 tcctgcgtta tagatggctc agatgatctg gcttcattaa aagattcact taagctttct
     1741 tctggtatat taaaagattc acttgtgctt tcttctggta cattaaaaga ctcacttgtg
     1801 ctttcttctg gtacactgat ttctattgag accgtatttg attgaatatc accgcttctt
     1861 gcggtcaatt cgtatttacc tgaatgctgg gaagaataag taatcagacg ttcgtaatca
     1921 ttgagttgct tcacttcccc ttcaatcaaa tcctcactct tatcaatgac caatgcattt
     1981 agcaggtctg gtgttcctct gactcgcaac tggaactgct cacccaaagc agcctcctct
     2041 tgatcaactt cgatcgtcag cgaacgctct ggctgttctg ctaaagactc agctagaacc
     2101 gttatgttcc caaattgatt gatcacaata agcataacta gaaacatact taacagttta
     2161 tgaaatttgt tcctcactat tactccccct tataattttg actaaacaac aaggtacccg
     2221 ataattttct aaaaagattc cccaactcaa aatggctgcg ttacctccct cgtattttcg
     2281 gttcaactca agcgttgcaa tgtaagctgt aaacaaagct aatgattgcc gattgtcttc
     2341 tgttgattga ttcgatagct ttgtatgact tttagttgtc cgtgtggctt aaccataatt
     2401 atgatttcac gattcctcct gccagtgcct tgcacaagct ggagcgtaac cctaatagtt
     2461 attttgttaa tgtacttata tacttttata cttttccatt agaaacaatc actaaatcgt
     2521 tgataccaag gtcagactct agaatttcat cagtgagtcc ctgtattggt taaccttcat
     2581 caatcccaaa aaaatatatc aatgcttagc atcaaaatga atatatagtt tagaatactt
     2641 gggattcatc gataactgat cgcttatttg gccatattat ttggcacttg gttcaactaa
     2701 acgcatttag gatgttcctt cattctcgtc atggatgttc tcaaaccgtc attactaata
     2761 aactgtccac cgaatcgatg gttcatttct tgcattttcc acgtccaatt actctttttg
     2821 ctctttattt gggcgtagtt tcactttctt aacttgtatc gtcttccatc aactccttaa
     2881 tcatcttctt agctttattg gctcggtttc cttgtaaaag gtatctacaa atagtgacaa
     2941 tctgtattaa atcttcatca ttttctgtaa tgtcaataat ttctatattc tgtaccatat
     3001 attgcaaagg gtttcactaa gttcataacc gaagcgaatt aaacaacttt tgtatagaat
     3061 aactaccttt tctatttttc cattggtaat tctatctatt aattggttta atccttttgt
     3121 tgtagttgat tccactacca agatgattct ctcgaatttt aaagcttgaa gatgtattgc
     3181 tatttttgct aactggcgta tttgcaacta tttttccctt ctattcttcc ttcattgatt
     3241 cccctctaaa attactatcc ataaaaaatt gcataaatga caatagtatt tatattagtc
     3301 atactg
//
LOCUS       DAZKLD010000197         3287 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.201, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000197 DAZKLD010000000
VERSION     DAZKLD010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3287
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.201"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            600..686
                     /locus_tag="ACG8KR_002779"
                     /pseudo
     CDS             600..686
                     /locus_tag="ACG8KR_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339775.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
     gene            1240..1464
                     /locus_tag="ACG8KR_002780"
                     /pseudo
     CDS             1240..1464
                     /locus_tag="ACG8KR_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877485.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            1724..1978
                     /locus_tag="ACG8KR_002781"
     CDS             1724..1978
                     /locus_tag="ACG8KR_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFQ0202086.1"
                     /translation="MGKELKVRKIGNSVGVILPSSLGLKSGDTIQAKQEGNLFILDTT
                     QIAKEHDRKLIEESFQDFEKGLTVSEIEMVKAFGKYGWSE"
     gene            1979..2296
                     /locus_tag="ACG8KR_002782"
     CDS             1979..2296
                     /locus_tag="ACG8KR_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFQ0202087.1"
                     /translation="MKEYTLVYSKSFQTSLETIIEEWENELALSPASIKRFVGAIQKA
                     LELTKNFPTMHEEVSEIYGMDIPTYRIVIGKSYALFYRIDHEKKEILVGRIFQSKQMK
                     LGF"
     gene            2855..>3287
                     /locus_tag="ACG8KR_002783"
     CDS             2855..>3287
                     /locus_tag="ACG8KR_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0202088.1"
                     /translation="MNILNEKRYVWFAGKKYITTFKRLSELMQQTVKSAMPEGYKFNF
                     VQSSVSTESIYFSILSESRLFHFRISSHVKKTDVDIYTFRTNNYKNLNDLSRSISEYL
                     KGNSYLKIRFSHILILLPYLNENFTLIQVDKSFIASGNQGNM"
BASE COUNT         1181 a          451 c          557 g         1098 t
ORIGIN      
        1 attaatactt aaattagata ctacctatat ttatattaag tactaccgtt ctaggaataa
       61 gataataaaa aaaaactacc aaattgcaat taaatactac ttaaaatgta aaaaataaga
      121 actacccaaa ttaggtagtt cttaataaga gaatatcaga taaatttgta caagtcataa
      181 aaaaattaat cttaaatttt taaatatata tacctgttaa ttataaacta ctttatttag
      241 gtagtacata attaacagat aaaactacat gcttgtagtt tgtctagcta atgagttgca
      301 tatgatttat ataaaggtgt gcagcataac ttagttgaag ttaagagaat agaataaata
      361 ttttttgttt tgctaataat taaacactac ctaaatatgg tagtgtttaa ttatataata
      421 ttcattttgt tacaaataat cgtattataa aagcataaca attgggataa ctatttttta
      481 gattatttaa gtactactta aattaggtag tacttaaatt ttggtttaag gtagtaggta
      541 attaagaaat aatatatatc ttcttttata atagaagggg agaggcctag ttaaaaacag
      601 cgacaattaa taacaatgta tttaatgctt cacaaaatcc atttggtgga caatgcattg
      661 ttattagcga tgatctaccg ttctaagtag ttttttctat tggttgcatt cgtgactaat
      721 tatggtataa ttatgagtaa caaagaggta tttcattata tcatcaaacc ccatcagcgt
      781 gtaggctgat ggggtttttt ttacgtttct atattataga attaactggt aggcttactg
      841 tcttttttgg caattttgca atcaagccac ttgcagatgt agtgtgcagt tacctctgca
      901 aatggcttga ttgcaaaaac aaaatcaaaa aggtatttca ttatatctcc tcctcccctt
      961 gccagattag gccggggtag cagacagcaa aactattata acataactga aatacctaaa
     1021 tgacatatac agatatgata atttatgtat tgatttttct aacaaaagca catattttaa
     1081 tatatactac ctattttagg tagtatatat ttttagatac aatatcgatc agttttgagg
     1141 aaatgaaatt ctatatattt tttgctacat agagttactt tcaatatttt ttaacgtaat
     1201 taggcagaag tcactcatcc tctataggtt tgtgatgaaa ttgctattga tccatttaag
     1261 taagagcaaa aaactggagg tttatcaggt aattggtcta atggatttaa gtatgcggtc
     1321 accgtgtata gagttgctta tgagattcaa gaaaataaag ttattccagt attgctatat
     1381 ggaacacata acaattttta tgaattagcc gagaacactc gcaactttat tcgtgtagat
     1441 gaatcggctt cggttgagtt ataactttag ctatatcaat caaccgaccg acatatgtta
     1501 aatctatata taataaaaat acctacttaa atggatgtta acggaagtgt atggtgagta
     1561 gttcactcgt attttgatat cgtttgtata tgttttcagc gttctaagtg gcttattttg
     1621 tccaataata atatggagtg gaatccctta agaaacaccg ttggcaattg tcgttataac
     1681 atgttataat ttatttatca aaaactaaaa gaagtgtgat aacatgggaa aagaactaaa
     1741 ggttagaaag atcggcaata gcgtgggagt gatccttcca agctctcttg gattaaaaag
     1801 cggagacact atccaagcca aacaagaagg caacttattc atattagata ctactcaaat
     1861 agcaaaagaa cacgatcgca aattgattga agaaagtttc caagactttg agaaaggttt
     1921 aacagtttca gaaattgaaa tggtaaaagc tttcggaaaa tacggctgga gtgaatagat
     1981 gaaagagtac acgctagttt attctaaaag tttccagacc agccttgaaa cgataataga
     2041 agaatgggaa aatgagcttg ctctttctcc agccagcata aaaagatttg taggtgcgat
     2101 ccagaaggct ttagaactaa ccaaaaattt tccaactatg catgaggaag tttccgaaat
     2161 ctatggaatg gatatcccaa catatcggat cgtgatcggc aaaagctatg ccttatttta
     2221 tcggatcgac catgaaaaaa aagagatact tgtaggacgt attttccaat ctaaacaaat
     2281 gaaactaggc ttttaacaat aattaggcaa aagtcgctca tcctctacag gtgggtgatt
     2341 aatgccgctc gatatggttg ttaaccgtcg gttactcgaa gattgatata taagacttag
     2401 taccttttta ttttgctcca tgttatggcc gaaggtctaa agatacaatc attagtctag
     2461 agaaaaatta agaaccgtag gaactacggg gatcgcttgg tgaataagag acactgctat
     2521 tagtgaaaaa ataagatatc aagtacgctc tattcccaaa aatttcgtga ctttactcat
     2581 gagaggttca agtagtatat ttttaatcaa aattgacgta acttttaata aatactacct
     2641 aaataaggta gtatttattt ttgtgtttta aaagaggaag tccaatatat ctatgggaaa
     2701 cttcttaaat attatgttgg tacatgaaaa tatacatccc tgctagacta tttacaacat
     2761 tttactttgt acgtattctt gttttaaaag gaatgggata aaatataggt acaataacta
     2821 tttgatgcta aatataatta atggaggaga gatagtgaac atacttaatg aaaaaagata
     2881 tgtatggttt gctggtaaaa aatatattac tactttcaaa agattatccg agttaatgca
     2941 gcagactgtt aaaagtgcta tgcctgaagg atacaagttt aattttgttc aatcttcagt
     3001 atctactgaa agtatctatt tttcaattct ttctgagagt aggctttttc attttagaat
     3061 aagttctcat gtaaagaaaa ctgatgttga tatatatacg ttccgtacaa ataattataa
     3121 aaatttaaat gatttgagta gaagtatcag tgaatatctc aaggggaata gctatctcaa
     3181 gattcgattt tcacatatat taattttact accatatttg aatgagaatt tcaccctaat
     3241 tcaagtagat aagtctttca ttgcctcagg taatcaagga aatatga
//
LOCUS       DAZKLD010000198         3259 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.202, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000198 DAZKLD010000000
VERSION     DAZKLD010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3259)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3259)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3259
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.202"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(108..605)
                     /locus_tag="ACG8KR_002784"
     CDS             complement(108..605)
                     /locus_tag="ACG8KR_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0033745 - L-methionine-(R)-S-oxide
                     reductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HFQ0202089.1"
                     /translation="MWTEKQKKDAYEILLLQQQGLLEAEDNWLANLANSSALLNETLP
                     ETVFAGYYLFDGNELILGPFQGRVSCTRIKMGKGVCGESAEKQETLIVDDVKTHANYI
                     SCDSAAQSEIVVPMVKEGNLIGVLDIDCGVTKGYDEIDQAFLEKYVDLLLANFNINAF
                     SFINN"
     gene            complement(735..1445)
                     /gene="budA"
                     /locus_tag="ACG8KR_002785"
     CDS             complement(735..1445)
                     /gene="budA"
                     /locus_tag="ACG8KR_002785"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047605 - acetolactate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0045151 - acetoin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HFQ0202090.1"
                     /translation="MTEKILYQHGTLGALMAGLMDGTETIAHILEKGTLGLGTLHGLD
                     GEVIFLDGVAYQGRSDGSVAQLDGSELTPYAAITDFTPDTSFSVSKTADGEQLKKDIL
                     VREAGENLFLAVKITGLFKNMHIRIMPKQEKPYRRLAKISESQPEFQQSNVQGTLVGF
                     FTPELFQGVAAAGFHLHFIDDTHTFGGHVMDFEVAEGKVEISRISSLVQHFPVDDPTF
                     VHETIDYADLAAEIQQAE"
     gene            complement(1458..3122)
                     /gene="alsS"
                     /locus_tag="ACG8KR_002786"
     CDS             complement(1458..3122)
                     /gene="alsS"
                     /locus_tag="ACG8KR_002786"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0034077 - butanediol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HFQ0202091.1"
                     /translation="MAEKMKQGSKSVVESLINHHVDYVFGIPGAKIDGVFNELEDQGP
                     ELIVTRHEQNAAFMAQAIGRITGEPGVVIATSGPGASNLATGLVTATAEGDPVLAIAG
                     QVKRSDLLKLTHQSMDNAALFQPITKYSAEIQDPETISEVIANAYRMAKSSKKGASFI
                     SIPQDVVDAPVQGNVIKPLSDPKLGSASADDIRYLAERIREAKLPVLLVGMRGSSEKE
                     TLAIRQLVEKTALPVVETFQAAGVISRELEAHFFGRVGLFRNQPGDMLLKRSDLVIAI
                     GYDPIEYEARNWNAEKDARIIVIDEAPAEIDPFMQPERELIGDISATLDLLTGSLEPQ
                     QVSEDAKEYLASLQAKLTERDIVQSKGEAGILHPLEVINTLQSKVTDDMTVTVDVGSH
                     YIWMARHFRSYEPRHLLFSNGMQTLGVALPWAISAALVRPNTQIVSVSGDGGFLFSAQ
                     DLETAVRKKLNIVHLIWNDGHYNMVEFQEKMKYQRASGVDFGPVDFVKYAEAFGAKGI
                     RATSVEELEKALEEGFATEGPVIIDIPIDYRDNEKLGETILPDQFY"
BASE COUNT          905 a          718 c          532 g         1104 t
ORIGIN      
        1 cttgtgattt tataaaaaaa taaaaaaacc gaaaaacaga aatgcccact gtctttccgg
       61 ctctttttat tcttgatttc tcttcttttt tgtttcaata gacgatttta gttattaata
      121 aatgaaaaag catttatatt aaaatttgct aataaaagat ctacatattt ttctagaaat
      181 gcttgatcga tttcatcata ccctttcgtt actccgcaat cgatatctaa aacaccgatt
      241 aaattacctt cttttaccat tggcactacg atttctgatt gtgcggctga atcgcaggaa
      301 atgtaatttg catgggtttt gacatcatca acgatcaatg tttcttgttt ttcagctgat
      361 tctccacaaa ctccttttcc catcttgata cgtgtgcaag aaactcgccc ttggaacggt
      421 cctaaaatca attcattacc atcgaataaa taatatcctg caaagacagt ctcaggcaaa
      481 gtctcattca acaatgcaga tgaatttgct aaatttgcta accagttatc ttctgcttct
      541 aataatcctt gttgctgtaa taataaaatt tcgtatgcat cttttttctg tttttctgtc
      601 cacatgataa aaaccgctcc tttttgatct atggagaaga ttatagctca gtttttttgt
      661 aaacgctagg ttttctcctc aaaaaaacga gatacgtcag gttaatcacc ttgaacgcat
      721 ctcgctgtct ttttctattc tgcttgttgg atttctgctg ctaaatcagc ataatcaatc
      781 gtttcgtgaa caaaagtcgg atcatcaact ggaaaatgct gtacaaggga cgagatgcgg
      841 ctaatctcaa cttttccttc agccacttca aaatccatta catgtccgcc aaatgtatgt
      901 gtatcatcga taaaatgcag gtgaaatccc gctgcagcaa ctccttggaa taattcaggc
      961 gtaaagaacc cgactaatgt tccttggaca ttgctttgtt ggaattctgg ttgagactct
     1021 gaaatttttg ctagtcttct gtaaggtttt tcttgcttcg gcatgatacg gatgtgcata
     1081 tttttgaata atccagttat cttcactgcc aaaaataggt tttccccggc ttctcttact
     1141 aatatgtctt ttttcaattg ttctccatct gcagtttttg agacagaaaa actagtatct
     1201 ggcgtaaaat ctgtaattgc tgcatagggg gtaagttcac ttccatctaa ttgggcaact
     1261 gagccatctg atcttccttg ataagctacg ccatctaaaa agatgacttc accatcaagc
     1321 ccatgcaatg ttcccaatcc cagagttcct ttttctaata tatgagcaat cgtttctgtt
     1381 ccatccatca agccggccat caaagctcct aatgtcccgt gctggtataa gattttttca
     1441 gtcatgtcga cacttcctta atagaattga tctggtaaga ttgtttctcc caatttctca
     1501 ttatcacggt aatcaatcgg aatatcaata attacgggtc cttctgtcgc aaatccttct
     1561 tctaaagctt tttctaattc ctctacactt gtagctcgga ttcctttagc gccaaaagct
     1621 tctgcatatt tcacaaaatc aactggacca aagtctacac ctgacgcccg ctgatatttc
     1681 attttttctt ggaactctac catattgtag tgtccatcat tccaaatcaa atgaacgata
     1741 ttcaattttt tacgtaccgc cgtttctaaa tcctgggcag agaataaaaa tcccccatcg
     1801 ccggaaactg atacgatctg tgtatttgga cgcaccaatg cagcagaaat cgcccatggc
     1861 aatgcaacac caagcgtttg cataccatta ctgaataaca aatgacgagg ttcataactt
     1921 ctaaagtggc gtgccatcca aatataatga ctacctacat ctacggtaac agtcatatca
     1981 tctgttactt ttgactgaag cgtgttaata acttctaaag gatgcaagat acctgcttct
     2041 ccttttgatt gaacaatgtc ccgctccgtt aattttgctt gaagagatgc taaatattct
     2101 tttgcatctt ctgacacttg ttgaggctct aatgaacctg tcaataaatc aagagtggct
     2161 gagatatcac cgatcaattc tctttcaggc tgcataaaag gatcgatttc agctggcgct
     2221 tcatcgatca caatgatccg cgcatctttt tccgcattcc agtttcttgc ttcatattcg
     2281 attggatcat agccaattgc aataactaga tcacttcgtt tcaaaagcat atctcctggt
     2341 tggtttctaa ataaacccac acgaccaaag aaatgcgctt ctaattcacg tgagatcact
     2401 ccagctgctt ggaatgtctc tacaacaggc aatgctgtct tttcaactaa ttgcctgatg
     2461 gctaacgttt ctttttcact tgatcctcgc atacctacta atagtaccgg tagttttgct
     2521 tctctaatgc gttcagctag gtaacggata tcatctgcag aagcagaacc taatttagga
     2581 tcgctcaatg gcttgatgac attaccttgg acaggtgcat ccactacatc ttgcgggata
     2641 ctgataaagc tcgcgccttt ttttgaactc ttagccatac gataagcatt ggcaatcact
     2701 tctgaaattg tttctggatc ttggatttct gcactgtatt ttgtgattgg ctggaacagt
     2761 gctgcattat ccatgctttg atgggtcaac ttcaatagat cgcttcgttt tacttgtccg
     2821 gcaattgcta aaactgggtc accttctgct gtcgctgtta ccaaacctgt agctaagtta
     2881 ctagcaccag gaccacttgt tgcgataact actcctggct ctcctgtaat acgtccgatt
     2941 gcttgtgcca taaatgcagc attttgttcg tgacgagtca cgattaattc aggaccttga
     3001 tcctctaatt cattaaatac accatcgatt tttgcacctg gtataccgaa tacataatca
     3061 acatgatgat taatcaaact ttctactaca gatttcgaac cttgtttcat tttttcagcc
     3121 attgaacatc gtccttttta tgctttattt ttaattatta gttagaaata atcacaaata
     3181 agtgatacag tttctaaacc tgtttcattc aaaagtatag catgttttat ttcatttgtt
     3241 tagccatatg tttcaaaaa
//
LOCUS       DAZKLD010000199         3255 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.203, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000199 DAZKLD010000000
VERSION     DAZKLD010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3255
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.203"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            156..1862
                     /gene="efbA"
                     /locus_tag="ACG8KR_002787"
     CDS             156..1862
                     /gene="efbA"
                     /locus_tag="ACG8KR_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding protein EfbA"
                     /protein_id="HFQ0202092.1"
                     /translation="MSFDGVFTHAMVNELRETLLSGRISKIHQPYENEIVLVIRSRGK
                     NHRLLLSAHPSYARVQITQIDYQNPDNPPNFVMMLRKYLDGAILEDIEQIENDRVIHF
                     HFAKRNELGDLQNIILIVELMGRHSTIVLVNRETGKILDAIKHIGSSQNTYRSLLPGV
                     EYVAPPKQEVLNPFSSEKEKIFQRLSQTELDPKAIQRQFQGIGFDIAQELTKRLLERP
                     NEKMVVWDEFFSAISHQPIPTFYETENKDFFTPIAYQVLSEQASAVTAYPTLSQLLDS
                     YYHEKAEKDRAKQQGGELIRKIENELKRNKNKLKKREQTLKESENAENYRRNGELLTT
                     FLTQVPRGAKEVVLPNYYEEDRPIKIALDPALTPNQNAQKYFHRYQKLKNAVKLIGEQ
                     IQEAKDEIQYLESVLSQLEIAGPMDIEAIKEELTSEGYLKKKNLKKQKRKKPSQPDQY
                     FSSDGTLILVGKNNLQNDQLTMKTAKKTDYWLHAKNIPGSHVIIKSDKPSDETITEAA
                     ELAAYFSKYRYSAQVPVDLVQVKHIRKPNGAKPGYVIYENQKTIIVTPEEEKITAMKQ
                     NR"
     gene            2095..>3255
                     /locus_tag="ACG8KR_002788"
     CDS             2095..>3255
                     /locus_tag="ACG8KR_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa5 family transposase"
                     /protein_id="HFQ0202093.1"
                     /translation="MNDSIKKMLRLIEKDLMITEVSYETFQKKKTLIVDAVFSPAPHT
                     CRNCGSTVVDGNGKVIVVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKNCQVSLTTVIRTLKEFKSYLPKQSK
                     RILPRVLMVDEFRSHASIEDKMSFICADGETGQLIDVLPTRKLPRLTSYFLGCANPEE
                     VKFLVTDMNAAYFQLTKRILPNAKVVIDRFHIIKHMNQAFNELRIREMNELRKVGQKS
                     QAEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLGFSTSLKEAY
                     PYFHELVEAFRDKDPDLFFSLLAELPETLDDGFREKLQNLLTYEEGITNAMIY"
BASE COUNT         1124 a          634 c          604 g          893 t
ORIGIN      
        1 acctaaaaat ttggatttca acgatagaaa cactgttttt tatgtaatta gtccaagatt
       61 tttctagact tttgctttac tattcggcca aattggtttt tcttcttttt ttcgttataa
      121 tgaatatact tatgaaaaga aatgaggaat gaaccatgtc ctttgatggt gtatttaccc
      181 acgcgatggt caacgaattg cgtgaaacgt tactatctgg ccggatctcg aaaatccatc
      241 agccttatga aaatgaaatc gtgctggtga ttcgttcacg cggcaaaaac catcgattat
      301 tattatctgc gcacccaagt tatgctcgtg tacagattac ccaaatcgat tatcagaatc
      361 ctgataatcc gcctaacttc gtgatgatgc ttcgaaaata tttggatggc gctattttag
      421 aagacatcga acaaatcgaa aatgatcgag tgatccattt ccactttgct aaacgaaacg
      481 agttgggtga tctacaaaat atcatattga tcgttgaatt aatgggacgt catagtacga
      541 tcgtcttagt taatcgagaa actggaaaaa tactggatgc gatcaaacac attggaagct
      601 ctcaaaacac ttatcgttct ttgcttcctg gtgtagaata tgttgctccg ccaaaacaag
      661 aggtcttgaa cccatttagc tccgagaaag aaaaaatctt tcagcggctt tcccaaactg
      721 aactagatcc aaaagcaatc caacgtcagt ttcagggaat cggtttcgat atcgctcaag
      781 agctgaccaa aagattactg gaacgtccta atgaaaagat ggttgtatgg gacgaatttt
      841 tctcagccat cagccatcag cctattccta ctttttacga aacggaaaat aaagattttt
      901 tcacaccgat tgcttatcag gtattgtccg aacaggcatc tgctgtaaca gcttatccaa
      961 cactcagtca attattagat agctattatc acgaaaaagc agagaaagat cgggcaaaac
     1021 aacaaggcgg agaactgatt cgaaaaatcg aaaatgaact aaaacgaaat aaaaataaat
     1081 tgaaaaaacg tgaacaaaca ctcaaagaat ccgaaaacgc tgagaattac cgtagaaatg
     1141 gcgaactact tactactttt ctgacacaag taccacgcgg cgcaaaagaa gttgttttac
     1201 ctaattatta tgaagaagat cggccaatca aaatagcact agatccggca ttgacaccaa
     1261 atcaaaatgc acagaaatat ttccatcgat accaaaagct gaagaacgct gttaagctga
     1321 ttggcgaaca aatccaagaa gccaaagatg aaatccaata tcttgaatct gtcttatctc
     1381 aactggaaat tgccggccca atggatatcg aagcaattaa agaagaattg acttcagaag
     1441 gatacctgaa aaagaagaac ttaaaaaaac aaaaacgtaa gaaaccatct cagcctgatc
     1501 aatacttttc ttctgacgga acgctcattc ttgttggtaa aaacaatctt caaaatgacc
     1561 agctgacaat gaagacagct aaaaaaaccg actattggct tcatgcaaaa aatatacccg
     1621 gttcgcacgt catcattaaa agtgacaagc cctcagatga aacaattaca gaagctgccg
     1681 agttagctgc ctatttttca aaataccgat actctgccca agtgccggtt gatcttgtac
     1741 aggtaaagca tatccgaaaa ccaaatggcg ccaagcctgg ttatgttatt tatgaaaacc
     1801 aaaagacgat tatcgtgaca ccagaagaag aaaaaattac agctatgaaa caaaacaggt
     1861 aatcaatgag tgtttctaga atcatatttg cgtactttcc cacaacagca attagttata
     1921 aattgtttgc tacaaaaaga ggagttgtga caaaattcgg ctctataatt tatgggactg
     1981 atgaatcaga attgattctt cagccccatt tttattttag gtattaaaaa acatatcgtt
     2041 ctctagtatg attaaatcac gacaaaataa acaactgaga ggacgatatg ctatatgaat
     2101 gattctatca aaaaaatgct gcgattaata gagaaagatt tgatgattac agaggtctct
     2161 tacgagacct ttcagaagaa aaagacgttg atcgtcgatg ctgttttctc acctgctccc
     2221 catacctgta gaaattgtgg ttctactgtg gtagatggaa acgggaaagt aattgtagtg
     2281 aaaaatggga agaaggaaac gattgtccgc tttgaacaat acaatcacat gcctttggtt
     2341 atgcgcttaa aaaagcagcg ctatacctgt aaaaactgtc gaacccattg gacggctcaa
     2401 agttattttg ttcaacccag acattcaatc gcgaatcatg tcagatataa aattgcttct
     2461 ttactgactg aaaaagtatc tttatctttt attgcgaaaa attgtcaggt atctttgacc
     2521 accgttattc gtacgttgaa agagtttaaa agctacttac caaagcaatc taagaggatt
     2581 ctccccagag tattgatggt tgatgaattt cgttcgcacg cttccataga agataaaatg
     2641 agctttattt gcgcagatgg cgaaacagga caattaattg atgtcttgcc tacgcgtaaa
     2701 ttgcctcgat taacaagcta tttcttaggc tgcgctaatc ctgaagaagt aaaattttta
     2761 gtcacagaca tgaacgccgc ctacttccag cttaccaaac gtattctgcc aaatgcgaaa
     2821 gtagtgattg atcggtttca tattatcaaa cacatgaatc aagcctttaa tgagttgcgt
     2881 atccgtgaaa tgaatgaact tcgtaaagta ggacagaaaa gtcaggcaga aaaactgaaa
     2941 aagaactggc gctttttgct aaaaaatcgt gcaaacatca accattatga atacaaaaca
     3001 tggaaaagtt ttcgagcacc aaaataccca tttcttacgg aagcgatgat gattgatcga
     3061 ttgcttggct tttctacgtc cctgaaggag gcgtatccct attttcatga attagttgaa
     3121 gcctttcgag acaaagaccc tgacttattt ttctccttat tggcagaact tcccgaaacg
     3181 ttagatgacg gctttcggga aaagctccaa aaccttctga cctatgaaga aggcatcacc
     3241 aacgcgatga tctat
//
LOCUS       DAZKLD010000200         3204 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.204, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000200 DAZKLD010000000
VERSION     DAZKLD010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3204)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3204)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3204
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.204"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            679..765
                     /locus_tag="ACG8KR_002789"
     CDS             679..765
                     /locus_tag="ACG8KR_002789"
                     /inference="COORDINATES: protein motif:HMM:NF033608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HFQ0202094.1"
                     /translation="MFTNFICSLLVGLLSALFSYWLNNRNKK"
     gene            complement(1067..1291)
                     /locus_tag="ACG8KR_002790"
     CDS             complement(1067..1291)
                     /locus_tag="ACG8KR_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000410576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0202095.1"
                     /translation="MEKFPYEYFQNLEKLEQAIQAIKFDLTDEKLLEYKNMTPERKRS
                     LEKLMQLVNEDGGISDAAFDAWSEFHGEYN"
     gene            1657..1941
                     /locus_tag="ACG8KR_002791"
     CDS             1657..1941
                     /locus_tag="ACG8KR_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0202096.1"
                     /translation="MDILVRNIDPKFVATIDKRCQELTNKTGEKWSRNKYLKVLIESD
                     FDRQLTTYKKDQFDLLLDKFIAIQTENTQILSEYVYTNNQIIEMLIGGMI"
     gene            1944..2360
                     /locus_tag="ACG8KR_002792"
     CDS             1944..2360
                     /locus_tag="ACG8KR_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0202097.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDINNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     EAKLNPSNRNRPKNHQKETSQTVTSRNFDPTNFLKR"
     gene            complement(2497..>3204)
                     /locus_tag="ACG8KR_002793"
     CDS             complement(2497..>3204)
                     /locus_tag="ACG8KR_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFQ0202098.1"
                     /translation="TELTVKYQNELNLVPLKNFNAKQMDLFFALCARMKDRGLKKVRF
                     SFEELKELSDYKMTATKAFVGDLEKLYKDMLNLTYRTEDEDSIRYFVLFTGFEINKTK
                     KFVEVSVNPDLEYIINGLTAEFSRFELSAFTSLRSTYAKTLFRLLMQYRSTGYYVVRI
                     EEFRSLMDIPEYYQMGNIDQKVLNPALRELKKYFNDLKVTKIKAKKGNKIAKLEFTFS
                     SLTSDLPKISLRNWLEE"
BASE COUNT         1065 a          599 c          500 g         1040 t
ORIGIN      
        1 tccgtttttt catccattcc tttcttcccc ccttacaaat aaataataac ctttaacaac
       61 caactcgtaa ataggttggt tgttattata caaaaggata ttagtactca gataaaaagt
      121 tgttagtact cagataaaaa gttgttagta ctcagataaa aagttgttag tactcagata
      181 aaaagttgtt agtacccttc tgtatccgtt gggagagtaa ggctcaactg ccccctaaaa
      241 agggttctaa aaaatcttta aaaaataaat aaaataaacg cgcgcgcgcg agcaaaattt
      301 cccgccaaaa aatcacccac tttttaaaat aaacaggttc gcgcatgata ttaaactgcc
      361 aatctagctc cctatggtcg ctgtaagaag gagtgatcgc tacgctctca cattttactt
      421 gcagcagtcg cctttaggct ccttcgccaa aaataattca ttagggactc ctttgtggcg
      481 tcctgagagc tcgtctaagc cgttctaaga caagattcga taaaaactat gtccttcgtt
      541 ctaaagcgcc ttacaggcgg tcagggcaag ccctaacaac cccaaggcaa taaaaaactt
      601 aggcttattt ttgctttttt attgtataat tgattgtcgc ctatccgaca gtcttgcgac
      661 tggaaggagg tgtgtttcgt gttcactaac tttatttgct cccttcttgt tgggctttta
      721 agcgctttgt tcagttactg gttgaataac cgaaacaaga agtaaaactt aattaaggcg
      781 actcagccca caaaaaaagc cccagagaat ccgacctctc tggggctttc gtgtgtttcg
      841 tgttcactta tttgcaatca aattataaca gaaataaata tttatttcaa aaatatccta
      901 caaaagtcta aaatccgtaa ttatttctta tatgcaacat ttacaaataa agtcgctctg
      961 agagctcgta tttttcgatt taagacgttt tatttagttt ttggtgtatt tcattggtcc
     1021 aagctaaaaa actcttagaa gcgattttag agccatttaa acccacttaa ttatattctc
     1081 catgaaattc gctccatgcg tcaaaagctg cgtctgaaat tccgccgtct tcgttcacta
     1141 gttgcattaa tttttccaac gatcgttttc tttcgggtgt catattttta tattccaaca
     1201 atttttcgtc tgttaaatcg aacttgattg cttgaattgc ttgctctaat ttttctaagt
     1261 tttgaaaata ttcgtaagga aatttttcca ttttttcttt ctccagtctc acaatttttc
     1321 tagaaataaa atcacttctt tgccgtctat actctcaaaa aaaatgtcgt ttgaaaaact
     1381 aaataaattt ttgatcgtgc atttttaaat ttggttatcg attacacgtg cagcactccc
     1441 ccccgaattt taaagagcat aaggaaacgt gtacccactt tctaaaaaaa gtgggtagat
     1501 aggctgtgcc tatcacgttt ctcagaaaaa aatttgatat cactttttcg ccatcgtggg
     1561 tagcttatgt atagcatttt ggcaaaaaac agctatacaa atatagtatt tgtatggtat
     1621 acataaatta tacataattg caaaaggaga ttttttatgg acattttagt tcggaatatt
     1681 gatccaaagt ttgttgcaac aatagacaaa cgttgccaag aactcacgaa taaaacagga
     1741 gaaaaatgga gtcgaaataa atatctaaaa gttttaatcg aaagtgattt tgaccgtcaa
     1801 cttacgactt acaaaaaaga ccaattcgat ttattgctcg ataaatttat tgctatccaa
     1861 acagaaaata ctcaaatatt aagcgagtat gtctacacaa ataatcaaat cattgaaatg
     1921 cttatcggag gaatgatata accatgagaa aaacttctgt ccaaaaacat gttactttgt
     1981 accctagtca tgttgacgtt ttagattcca tcatcgaaaa aaatccagat atcaataact
     2041 actcggaagc cattcgcttt ttatgtatga gttatgaacc cgataccaac acgaccacta
     2101 ccagcaatca ggcaaaacta aatgctatgg gaaaagaaat ttccatcatg actgaaatca
     2161 tcgcagaatt tgctgattta tatctaaaag atacaggcgt tcttattggc acaaattcta
     2221 aagtatatca agaagtgaaa gaaaaaatcg aagctaaact aaatccatca aatcgaaatc
     2281 gaccaaagaa tcatcaaaaa gaaaccagtc aaacagtcac ttctagaaat ttcgacccaa
     2341 caaattttct aaaacgataa aaaacagata ggagttattc ctatctgttt tttatttaga
     2401 agccccatac aatatgctag aatgtactag caatcttagt cggttgtaag ggacttgtct
     2461 acttgcttaa tgtaaggggg gcgagttctt ttttatttat tcttctaacc agttacgcag
     2521 agaaatcttt ggcaaatctg acgttaggct tgaaaaagta aattctaatt tggcgatttt
     2581 attgcccttt tttgctttta tttttgtgac tttaagatca ttgaagtatt tttttagttc
     2641 tcgtaatgca ggatttaata ctttttgatc aatgttaccc atttgatagt attctgggat
     2701 atccattaag ctcctaaact cttcaatacg aactacataa taaccagtag aacggtactg
     2761 cataagcagt cggaatagag tttttgcata cgtagaacgc aacgacgtga aagcagataa
     2821 ttcaaatcta ctaaattcag ctgttaaacc attaataata tattctaggt ccggattaac
     2881 actgacttca acaaactttt ttgttttatt gatttcaaaa ccagtaaaca aaacaaagta
     2941 gcgaatagaa tcctcatctt cagtacggta agtaagatta agcatatctt tatagagctt
     3001 ttctaaatca ccgacaaagg cttttgtagc agtcatctta taatcactta actcttttaa
     3061 ttcctcgaaa gaaaatctaa cttttttcaa accacgatct ttcatacgag cgcataatgc
     3121 aaaaaataaa tccatctgct tagcattgaa atttttcagc ggtactaaat tcaattcatt
     3181 ttgatatttg actgttaatt ccgt
//
LOCUS       DAZKLD010000201         3199 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.205, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000201 DAZKLD010000000
VERSION     DAZKLD010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3199)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3199)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3199
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.205"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(251..619)
                     /locus_tag="ACG8KR_002794"
     CDS             complement(251..619)
                     /locus_tag="ACG8KR_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HFQ0202099.1"
                     /translation="MEKFQPIINQIKEKEHQQKIKDLFAKITETYPQLDTTVKWNQPM
                     FTDHGTFIIAFSFAKNHFSIAPEKAAIRALEKNIQEAGYVYTDNVIKVPWKSVINWEL
                     IEQLISFNIEDKKGHDKFWR"
     gene            769..1251
                     /locus_tag="ACG8KR_002795"
     CDS             769..1251
                     /locus_tag="ACG8KR_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HFQ0202100.1"
                     /translation="MIDLAKKFLHENIYFELYHHRAIYTNEDALVIKKEQGFTGTETK
                     SLFLKDKKDNNYVYLTFTTKQTDFKALGKIVGKRLSIVSSERMEEQTGQKQGAVSPFG
                     YEKQIPIIIDEELLSNEKLVFAPGRPDQTMVIQTVDLNKIIQLLEMPIFLMPANEKNN
                     "
     gene            1288..2298
                     /locus_tag="ACG8KR_002796"
     CDS             1288..2298
                     /locus_tag="ACG8KR_002796"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S66 peptidase family protein"
                     /protein_id="HFQ0202101.1"
                     /translation="MISYPLLERNATIGVTAPSSGVSEEHHSLLESALKRMEEKSFQV
                     VTGKTLWTQDKEKSGPARKRADEFNQMMQDENIQMIFPPWGGELLIEILDFVDFDALQ
                     PKWIVGYSDISTLLLAVTLKTGIATAHGTNLIDLRGEYLDETTAMWESVLKTREGESI
                     LQQPSEKYQKEWPHEHSSSSVFHLTEPTNWQSVSGDAVRMKGRLLGGCIDTISHLIGT
                     PYGDVQSFKRKYNDGKPILWFLENCELSSVDLRRSLVQMKLAGWFDGSSGIMFGRSAA
                     EDLLDYKMKDVYEDLSKELKLPILYDIDCGHVPPQLTLINGAYAEVAVEDRKATVLQT
                     FI"
     gene            complement(2413..3126)
                     /locus_tag="ACG8KR_002797"
     CDS             complement(2413..3126)
                     /locus_tag="ACG8KR_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0202102.1"
                     /translation="MKKLGFILLLVSLFLVGCTSPSKENSDPSATTDKSSIKQTSSAE
                     KSSTQKSASTETNTSSTSSKVSEPNTSSTSSSTVETNISSSTIDTTSSQSSQSIDPLA
                     GYSDLQIEYARVWLAIRGNTFRDTSGDFELHVIHYPAGTPIAPYDAGSAVYPKDVVML
                     TGKYSYQGVLVYSSNHDGTITSYPVPSHWQMPADQTSYPAFIRETTQEIIDNASVVAI
                     PTGNPNDVKQLINVIVMDN"
BASE COUNT         1079 a          517 c          621 g          982 t
ORIGIN      
        1 tttctcgtat tttttcctta ttcaaaagtt ttgcaggaaa attgctgaaa aaataatttt
       61 actaagtgga aaattcaaag ccattcaatc agtgattttt ttcagcacaa gaaagactcc
      121 ttataaaaat agcagatact ttgtcaatta agtgtttaac cagggatgat aaaaaggata
      181 ggattgaaca aaacctgtat aagctgatga atagtttcct cgccagtaaa aggtttattc
      241 gtataaagct ttatcgccaa aatttatcat gacctttttt atcttcaata ttaaatgaaa
      301 tcaattgttc aatcaattcc cagttgatta cacttttcca aggaactttg atgacgttat
      361 ccgtatatac gtagcctgct tcttggatat ttttttcaag ggcacgaatc gctgcttttt
      421 ccggtgcgat agaaaaatga tttttagcaa aactaaatgc aataataaag gtaccatggt
      481 cagtaaacat aggttggttc cattttacag ttgtgtctag ctggggatac gtttctgtta
      541 tcttagcaaa aaggtctttg atcttttgtt gatgttcttt ttctttgatc tgattgatga
      601 tcggctgaaa tttttccatt ctaaaacgcc tccattaagt ggtttgattt attttaccag
      661 aaaaacaata gttatgtata aaaaacgaac aaaacacaag taatgttgta accgctatat
      721 ttatatgata agatgtctca aataataaaa tagaaaagag acataaaaat gattgacttg
      781 gcaaagaaat tcttacacga aaatatatat tttgagttgt atcatcatcg agccatttat
      841 acaaatgaag atgctttggt aataaagaaa gaacaaggat ttacaggtac agagaccaaa
      901 agcttattct taaaagataa aaaagataat aactacgtat atctgacttt tactacgaaa
      961 caaacggatt tcaaagcatt agggaagatc gtcggtaaaa ggctttctat tgtttcatca
     1021 gaacgcatgg aagaacaaac gggacaaaag cagggcgctg tttctccgtt tggttatgaa
     1081 aaacagattc cgattattat tgatgaagag ctcttatcaa atgaaaaact tgtttttgca
     1141 ccaggtagac cagatcaaac gatggtcatc caaacggttg acttaaacaa aatcatccag
     1201 ctacttgaga tgccgatatt tttgatgccg gcaaacgaaa aaaacaatta accaagtaaa
     1261 atagtgcata cttggaggtg aagcaacatg ataagttatc cattattaga aagaaatgca
     1321 acgattgggg ttactgcacc atcctcaggc gtatcagagg aacaccattc gttactcgaa
     1381 tcagctttaa agagaatgga agaaaaatct ttccaagtag ttactggaaa gacgctttgg
     1441 acgcaagata aggaaaaatc aggtccagca agaaaacgag cagatgaatt caatcaaatg
     1501 atgcaagatg aaaatatcca aatgatcttt ccgccttggg gaggagaatt attgattgag
     1561 attctcgatt ttgtcgattt tgatgctcta caaccaaaat ggatagtagg ttattcagat
     1621 attagtacgt tgctacttgc tgtcacatta aaaacaggga tagccactgc acatggaacc
     1681 aaccttattg atttaagagg agagtacttg gatgaaacga cagcaatgtg ggaaagtgta
     1741 ctgaaaacaa gagaaggaga atcgattcta cagcaacctt ctgaaaagta tcaaaaagag
     1801 tggccccatg agcattcatc atcttctgtt ttccatctca ctgaaccaac aaattggcaa
     1861 tcagtcagcg gagatgcagt aaggatgaag ggacgattat taggtggctg tattgatacg
     1921 atcagtcatt taatcggtac cccttatgga gatgtccaat cttttaaaag aaagtataat
     1981 gatggaaagc cgatcttgtg gtttctagaa aattgtgagc tatcgtcagt tgatttaaga
     2041 agatcacttg ttcagatgaa actggcagga tggtttgatg gcagctcggg gatcatgttc
     2101 ggtagaagtg cagcagagga tttgttagat tataaaatga aggatgtata tgaagatctg
     2161 tcaaaggaat taaaactacc gatcttgtat gatattgatt gtggtcatgt accgccacaa
     2221 ctgacattga tcaatggtgc ctatgcagaa gtagcggtag aagatagaaa agcgactgtt
     2281 ttgcaaacgt ttatttaaaa aattttttcc tgccttggag tttatcaaag accaacaaat
     2341 ctaaatagaa aagaaaatga aagaagctga cagtattttg ccagcttctt tttgtatgat
     2401 ctattagaaa ttttaattat ccataactat cacattgatc agctgtttca catcatttgg
     2461 attacctgta ggaatagcta ctacagatgc attatcaata atttcttgag ttgtttctct
     2521 aatgaacgct ggataagatg tttgatctgc aggcatttgc caatgtgaag gaactggata
     2581 actagtgatt gtcccgtcat ggttacttga atacactaaa actccctggt aactgtattt
     2641 cccagtgagc ataaccacat ctttcggata gactgcactc cctgcatcat aaggggcgat
     2701 aggtgttcct gcaggataat gaataacatg tagttcgaaa tcacccgatg tatctctaaa
     2761 agtattacct ctaattgcta gccaaacacg tgcgtactca atttgtagat cagaatatcc
     2821 tgctaacgga tctattgact gagaagactg tgaggaagtt gtgtctattg ttgaagaaga
     2881 aatatttgtc tcaactgtac tactagatgt agaagaagta ttgggctctg agactttact
     2941 gcttgttgaa gatgtattcg tttcagtaga agctgatttc tgagtacttg atttttcagc
     3001 tgaactggtt tgtttgattg aacttttgtc tgtggtcgca gatggatcac tattttcttt
     3061 actaggagac gtacatccta caaggaataa agagaccaac aataaaataa aacctaactt
     3121 tttcatataa aatacctcat tattttttta ataccagtat agcataatcc tgaatttttg
     3181 aatccgtaaa accttgtct
//
LOCUS       DAZKLD010000202         3184 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.207, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000202 DAZKLD010000000
VERSION     DAZKLD010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3184)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3184)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3184
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.207"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..456
                     /locus_tag="ACG8KR_002798"
     CDS             <1..456
                     /locus_tag="ACG8KR_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0202103.1"
                     /translation="TISFEKSYNEMEFKKGNELIVNFADLCSGECLINDKSVMSFGDS
                     QYIRNFKSKILAMKSKEQSRGVFSKGGHGRKRKLDQDRWEAIKKRESNFVKTYNNQCS
                     AKIVKTAIRNKAQIIRIIEPDKAYGEWAYYQLKEQLVNKGKRYGIEVRS"
     gene            1306..2631
                     /locus_tag="ACG8KR_002799"
     CDS             1306..2631
                     /locus_tag="ACG8KR_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HFQ0202104.1"
                     /translation="MFDYSREPVNDYFLIDMKSFYASVECIERNLDPMTTPLIVMSRS
                     DNTGSGLTLASSPTAKKKYHITNVSRATDLPYPLPKELHIVPPRMNLYIKKNMEVNQI
                     FRKYVADEDLLIYSIDESILKVTKSLNLFTSSGTRSERRRELAKIIKDEIQKETGLIA
                     TVGIGDNPLLAKLALDNEAKSSKEFIAEWTYETVPEKIWKINEMTDFWGIGSRMKKRL
                     NHMGIMSIEDLANWNPYTIKSQLGVIGLQLYFHTNGIDRTDIAIPPEPTKEKSYGNSQ
                     VLPKDYTRREEVELVVKEMAEQVAIRIRQHNCKTGCVRLAIGTSILEEKSGFSHQMKI
                     PITDNTKELQNYCLFLFNKFYEGQEVRHIGVTYSRLVYTDSLQLDLFSDPRKKIAEEN
                     LDRIIDKIRQKYGFTAIIHASSMLEGARSITRSTLIGGHAGGLGGLHND"
     gene            2624..2971
                     /locus_tag="ACG8KR_002800"
     CDS             2624..2971
                     /locus_tag="ACG8KR_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0202105.1"
                     /translation="MIEYNDRGRLKWSGFYLSDHTERINADNVQRANHNLAKEQMTTE
                     EIAKILRTAVIKNRPVCIQKEERNAEGYYPDDVVGKIIGYDELGVFVEEEKVHYDEIR
                     NVSFSELLKWSQI"
     gene            2968..3150
                     /locus_tag="ACG8KR_002801"
     CDS             2968..3150
                     /locus_tag="ACG8KR_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0202106.1"
                     /translation="MNIIKKYEFGYITYEQLIEELWGYGQQLINEVGIDCFCFYIESG
                     AGYHKNRYYIFPYPPK"
BASE COUNT         1222 a          427 c          610 g          925 t
ORIGIN      
        1 acaatttctt ttgaaaaaag ctataatgaa atggaattca agaaaggtaa tgaattgatc
       61 gtaaactttg cagatttgtg tagcggtgaa tgtttaatta atgataaatc tgtaatgtct
      121 tttggtgata gccaatatat aagaaacttc aaaagtaaaa ttttagcaat gaaatcaaaa
      181 gaacaaagcc gaggagtctt ttctaagggc ggtcatggtc gaaaaagaaa attagatcaa
      241 gatcgatggg aagcaataaa gaaaagagaa agtaattttg ttaaaactta taataatcaa
      301 tgttctgcaa aaatcgttaa aactgctatt cgcaataaag ctcaaattat tcgtatcatt
      361 gaaccagata aggcttatgg cgaatgggct tactatcaat tgaaagaaca actagtaaac
      421 aaaggaaaaa gatatgggat agaagtcaga agttagtaaa ttaaacaatc gttgatttgg
      481 caacgaaata gtgagggaaa atccactcac tgaagggtaa actccgtcaa gttgtgtgac
      541 cagacggaag gacgaatgtt gttgaggttt tagaaccata taaaacagca tgtttctcaa
      601 atttttagga taagggaaat tgctagcagt ttgttttaga accatataaa acagcatgtt
      661 tctcaaatga gtactttaaa ttaattgatg gcgttcctgc tttagaacca tataaaacaa
      721 catggattta aaacaagaag taagtcagtt ctccttttac tttagtaagg aaaaataaag
      781 tattctcaat aaaacttaaa ataagcaaac ctttgtttta atagctaatc gaagtaaaac
      841 ccaaagccat atgaacccct gaatcagcga aagtttatga tgatttggat tactaatagt
      901 ttaggaatta gatggtaaat ctaaattaaa caatcgttga tttggcaacg aaatagtgag
      961 ggaaaatcca ctcactgaag ggtaaactcc gtcaagttat gtgaccagac ggaaggacga
     1021 atgttgttga ggttttagaa ccatataaaa cagcatagtt ctcaaattgt ttaaaatgat
     1081 tgtcaagtta aaaaaatctg ggatcatata aaatagtata gttctcaaat atgattagaa
     1141 accctattaa agagcaatat taagccaact tacttaaatc gtttaattaa gaacaataat
     1201 ctcttttagt cagactttta gtctgacttt tttgtatttc caaaatttat tctttacaaa
     1261 aacgaacata cgtttgtata attgcttgtg aatgggggaa taaaaatgtt tgattacagt
     1321 agagaaccgg tgaatgacta ttttttaatc gacatgaagt ctttctatgc tagcgtggaa
     1381 tgtattgaac gaaatttaga cccaatgact acaccgttaa ttgtcatgag tagatcggac
     1441 aacactggtt caggtctgac tctagcttct tcacctactg caaagaaaaa atatcatatt
     1501 actaatgtta gtagagctac tgatttacct tatccattac caaaagaact tcatattgtt
     1561 ccaccacgaa tgaacttata tataaaaaaa aatatggaag ttaatcagat ttttagaaag
     1621 tatgtcgcag atgaagattt attgatttat tcgattgatg aatcaatttt aaaagtgaca
     1681 aaatccttaa atttatttac tagttcagga acaagaagcg agagaagaag agaattagct
     1741 aaaataatca aagatgagat tcagaaagaa acaggtctaa tagcaacagt cggaatagga
     1801 gataacccat tattagctaa acttgcactg gacaatgaag cgaagagtag taaagaattt
     1861 atagctgagt ggacttatga gactgtacct gaaaaaatat ggaaaataaa cgagatgact
     1921 gacttttggg gcattggatc acgcatgaaa aaaagattga atcatatggg aattatgagt
     1981 atagaagatt tagcaaattg gaatccctac acaattaaaa gtcagttagg tgtaatcgga
     2041 ctccagttat atttccatac caatggaatt gatcgtacgg atattgctat tccaccagaa
     2101 ccaaccaaag aaaagtctta tgggaattca caagttctac caaaagatta tacgaggaga
     2161 gaagaagtag aactagtagt caaggagatg gcagagcaag tagccattcg aattagacaa
     2221 cacaattgca aaactggttg tgtacgctta gcaattggta cgtcaatttt ggaagaaaaa
     2281 tcaggtttct cgcaccagat gaaaatacca attacagata atacgaaaga attacaaaat
     2341 tattgtcttt ttttattcaa taagttttat gaggggcagg aagtacgaca tataggagta
     2401 acttatagca gattagtata cacagattcc ctacaattag atttatttag tgatccaaga
     2461 aaaaagattg ctgaagaaaa tttggatagg attattgata agattagaca aaaatatgga
     2521 tttacagcta ttatacatgc ttcatctatg ttagaaggtg caagaagtat tacgagaagc
     2581 actttaatag gaggacacgc aggaggatta ggaggtttgc ataatgattg aatataacga
     2641 tagaggacgt ttaaaatggt cgggatttta tttgtctgat catactgaaa gaatcaatgc
     2701 agacaatgta caacgtgcta atcataattt ggcaaaagaa caaatgacga ctgaagagat
     2761 tgcgaaaata ctgagaactg ctgtcataaa aaatagaccc gtctgcattc aaaaagagga
     2821 acgaaacgct gaaggttact atccagatga tgtagtagga aaaattattg gatatgacga
     2881 gcttggtgta tttgtggaag aagaaaaagt tcactatgat gaaattagga atgtttcttt
     2941 ctcggaatta ttaaaatgga gtcagatatg aacattatta aaaaatacga attcggttat
     3001 attacgtatg aacagctaat agaggaatta tggggatatg gtcaacaatt aattaatgaa
     3061 gtggggattg attgcttttg tttctatatt gaatccggag caggatacca taagaacaga
     3121 tactatatat ttccatatcc accaaaatag tctcttagat atttttaaat tttctccaaa
     3181 tact
//
LOCUS       DAZKLD010000203         3175 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.208, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000203 DAZKLD010000000
VERSION     DAZKLD010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3175)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3175)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3175
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.208"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(61..2424)
                     /locus_tag="ACG8KR_002802"
     CDS             complement(61..2424)
                     /locus_tag="ACG8KR_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase"
                     /protein_id="HFQ0202107.1"
                     /translation="MDYSSKEYFDKMTAWWRAANYLSVGQLYLKDNPLLRRTLKPEDV
                     KKHPIGHWGTIPGQNFIYVHLNRVINKYDLNMFYIEGPGHGGQVMVSNAYLDGSYTEI
                     YPEVTEDETGMQKLFKRFSFPGGIASHAAPETPGSIHEGGELGYSLSHAVGAVLDNPE
                     VISAVVIGDGEAETGPLAGSWFSNVFINPVIDGAVLPILHLNGAKIANPTILARKSDG
                     ELANYFNGLGWEPFFIEGNDPEKLNPVMAEKMDQAIEKIKSIQKEARLKTATDVVMPK
                     WPVLIVRTPKGWTGPKEWDGEPIEGTFRAHQVPIPVDQEHMDHADALLRWLKSYEPEK
                     LFDAQGRILEEIREIAPTGDQRMAKNPITNGGIDPRPLIMPDWKKYTLQFEKPGSVKA
                     EDMTELGKFVREIIEKNPENFRIFGPDETKSNRLNQVFKTTNRQWMEKIEPENDEWLS
                     PSGRVTDSQLSEHQDEGFLEGYVLTGRHGFFASYESFLRVVDSMLTQHFKWIRKSHDL
                     SWRKDYPSLNLIASSTVFQQDHNGYSHQDPGILTHLAEKKAEFIREYLPADANTLLAV
                     MDKAFRSSEKINLIISSKHPRAQFYSAEEAAVLVNEGLKIIDWASTAKEEEPELVIAA
                     AGTESNLEALAAVTLLLEEFPKLKIRFINVVDLLKLRHPSQDPRGLSDEEFDQYFTKD
                     KPILFAFHGYETLVRTIFFDRHNHHLMIHGYKENGDITTPFDMRVVNELDRYHLAKDA
                     ALKIKGSQAEDFAEKMDQKLQEHQNYIRENGIDLPEVLDWKWKNLDQ"
     gene            2589..3093
                     /locus_tag="ACG8KR_002803"
                     /pseudo
     CDS             2589..3093
                     /locus_tag="ACG8KR_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035543.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          860 a          662 c          574 g         1079 t
ORIGIN      
        1 cttaatttga caaataatac aatgtaaaaa attctgtttt gcgggatcga ttttatcatt
       61 ttattggtca agatttttcc atttccaatc caatacttct ggcagatcaa taccattttc
      121 acgaatatag ttttgatgtt cttgtaattt ctgatccatt ttttccgcaa aatcttctgc
      181 ttgacttcct ttgattttta aagctgcgtc ttttgccaaa tgataacgat caagttcatt
      241 tactacacgc atgtcaaatg gtgtcgtgat atctccattt tctttgtagc catgaatcat
      301 taaatgatgg ttatgacgat caaagaaaat cgtgcggacc aatgtttcat aaccgtggaa
      361 agcaaataag atcggtttgt cttttgtaaa atattgatca aattcttcat cgctcaaccc
      421 acgcggatct tgacttggat gacgtaattt caataaatct acgacattga tgaaccgaat
      481 ttttagtttt gggaattctt ccagcagcaa agttacagct gctaatgctt ctaagttaga
      541 ttccgttcct gcagcagcaa tgaccaattc aggttcctcc tcttttgctg tcgaagccca
      601 atcaatgatt ttcaagcctt cattcactaa gacggctgct tcttctgctg agtaaaattg
      661 agcacgcgga tgtttggaag agatgatcaa attgattttt tctgacgagc ggaatgcttt
      721 atccatgact gcgagcaatg tatttgcatc cgcaggcaga tattcacgga tgaactccgc
      781 cttcttttct gccaaatgtg tcaatattcc tggatcttgg tggctatatc cattgtgatc
      841 ttgctggaag actgttgagc ttgcaatgag attcagcgaa ggataatctt ttcgccaaga
      901 aaggtcatgt gatttccgta tccatttgaa gtgctgagtc aacatagagt caaccactcg
      961 caagaatgat tcgtaacttg caaaaaaacc atgccgtcca gtcagaacgt acccttctaa
     1021 gaacccttca tcttggtgtt ccgataattg actgtcggtg acgcgaccag atggcgaaag
     1081 ccattcatca ttttctggtt cgattttttc catccattgg cgatttgtcg ttttgaaaac
     1141 ttgattcaat cggtttgatt ttgtttcatc tggtccaaat atacggaaat tttctggatt
     1201 tttctcaatg atctcgcgaa cgaatttacc aagttcggtc atatcttctg ctttaacgga
     1261 gcctggtttt tcaaattgca aagtatattt tttccaatct ggcataataa gcggtcttgg
     1321 atcgattcca ccattggtga taggattttt tgccattctt tggtcaccag taggcgcaat
     1381 ttctctgatt tcttctaaaa tacgtccttg tgcatcaaat aatttttctg gttcatagga
     1441 tttcagccaa cggagtaaag catctgcatg atccatatgt tcttgatcta caggaatcgg
     1501 tacttggtgt gctcggaagg ttccttcaat tggttccccg tcccattctt ttggaccggt
     1561 ccatcctttt ggtgtgcgta cgatcaatac tggccatttt ggcatcacga catctgttgc
     1621 tgtcttcagg cgagcttctt tttggatgga tttgattttc tcgattgctt gatccatttt
     1681 ttcagccata acaggattta atttttctgg atcatttcct tcaataaaga aaggctccca
     1741 gccaagtccg ttaaagtaat ttgctaattc tccatctgat ttacgtgcca agatcgttgg
     1801 gttggctatt tttgcaccat ttaaatgaag gatcggtaaa acagctccat ctatcactgg
     1861 attgataaag acatttgaga accagcttcc agctaaagga ccggtttctg cttcaccatc
     1921 accaatcacg acagcagaga ttacttcagg attgtctaat acagctccga ctgcatgtga
     1981 aagagagtat ccaagctctc cgccttcatg gatagaacct ggtgtttctg gtgctgcatg
     2041 agaagcaatg cctccaggaa atgaaaatcg cttgaataat ttttgcattc ctgtctcatc
     2101 ttccgttact tctggataga tctctgtgta acttccatct aaataagcat tcgataccat
     2161 gacttgtccg ccgtgtcctg gcccttcgat ataaaacatg ttcaaatcat atttattgat
     2221 gactcggttc aaatggacat aaataaaatt ttggccagga atcgttcccc aatgaccgat
     2281 tggatgtttt ttgacatctt ctggtttcag tgtacgtctc agtaaggggt tatctttaag
     2341 ataaagttgc ccaacagaaa gataattagc agcacgccac caagccgtca ttttatcaaa
     2401 gtattcttta ctagaataat ccatacataa cactcctttt cattattact cttattctag
     2461 cgcagatact cccaattgaa acctaataag cacttctgtt catttgtttg gattttatat
     2521 ctagccaaat gaacaggtat aaaaaaccac gaaatttttc ctttcgctga cgttttttac
     2581 attgatccaa aaaacttgtc gttatcacaa tggctgttcc gcagttcatt tcgctcctca
     2641 tcatgcataa tttgctgaat gacgctaggc cagttaacac attgttggaa aactggggct
     2701 ggatcgagca attcctttcc tgattgatcc gttttatgta aaagtctctg ttatctttgt
     2761 caatatgtga attggtatcc cagctactat gctaatttct acagggatta tccaaaattt
     2821 ggatcgtgac caaatcgaag cggcgtaatc ggaaatatca ataacttcaa cgtgattttt
     2881 cttttaactg tcaacggacc gacaaactca aacttttatg gtgcaggttc cactgatctt
     2941 ctagttactt ggctatacct gactgtcact aaaatagatt acaatctagc ttccgttatc
     3001 ggtatcttga tctttatcta tatgcaaaaa tttatatact atagaaaaaa gaaagtcgaa
     3061 ttttttactt ttctttttct cttttttggt tagctgacac gctaccagac taaagtcatg
     3121 gaactcttag gtaatacgtc ttaatgaaaa caaaaaaatc ccgcaacccc tgatt
//
LOCUS       DAZKLD010000204         3086 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.209, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000204 DAZKLD010000000
VERSION     DAZKLD010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3086)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3086)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3086
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.209"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..211
                     /locus_tag="ACG8KR_002804"
     CDS             <1..211
                     /locus_tag="ACG8KR_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HFQ0202108.1"
                     /translation="VTGGEGWYQEEGKPARRLMAGDVVVTHKGIKHWHGATKDSWFEH
                     LAITAGNSEFLEPVTDEVYEGLENQ"
     gene            complement(303..653)
                     /locus_tag="ACG8KR_002805"
     CDS             complement(303..653)
                     /locus_tag="ACG8KR_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0202109.1"
                     /translation="MEKELSKRIIDELLTYLYSHEITEIRIGVEFAEEGLFIEIQGTA
                     ETQPEDLDVLADLLNNPRDPSMEIYYQELLGNPRHDREDYHLLGSLIDDVEIMYAQPI
                     INIKIFRRASHSYY"
     gene            complement(619..1950)
                     /locus_tag="ACG8KR_002806"
     CDS             complement(619..1950)
                     /locus_tag="ACG8KR_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HFQ0202110.1"
                     /translation="MENETDVIYIHPQKRIVSQKRKYFYLGFTGVFFLFIGLLSNTPT
                     DNWSGLLTILTSPSNLLTDYFALGVFGSAFINVGILTLLSVLLAYRHKVILNGPLFAS
                     ILTVTGFSFFGKNLYNSISIILGVYLYAVFVNKPFSQYIMIGLFGSVLSPVVSYITFG
                     MRFPLLIGILLGNLAGIAIGILLPPLAAQTLVFHRGFTLYNIGFTSGLIAMTFTAVLR
                     LFSYSIVENTLVFNEYHFPLIWIIFGFFSLTVGIGFYYNSFRLSGIREIFDSSGKLTT
                     DFIANSGIGATLINMGLVGLMLSSYVLLVGGQLNGPVIGAILSAVGFSAFGCHLKNSF
                     PILVGIFIASLFGTFHEITSTGMLVAAVFGTGLAPISGFYGSFYGVIAGVLHIALVHN
                     VSTLHGGLNLYNSGFSTGFVAGILVPILDNFTAVRKEKKTLGKRIIKKNHR"
     gene            2206..2772
                     /locus_tag="ACG8KR_002807"
     CDS             2206..2772
                     /locus_tag="ACG8KR_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFQ0202111.1"
                     /translation="MRRKVYTKDQILKAAYDVVAKEGFKGFTARNIAKKMGISTQPIY
                     LEFKNMEDLKNTLLDSIFEQLKEDVFPIERTGDKIIDLGLNYIYFAKKHHSLYMALYI
                     DEHGDGKKMHDFSYNYFREIVKEDERYNQLSDEYVDALHTGTWVAVTGLASLMSSNIM
                     HPTEEQIIALIKRTIDSILALDNPEIIR"
     gene            2917..3004
                     /locus_tag="ACG8KR_002808"
     tRNA            2917..3004
                     /locus_tag="ACG8KR_002808"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2949..2951,aa:Ser,seq:cga)
BASE COUNT         1135 a          541 c          584 g          826 t
ORIGIN      
        1 tgtgaccgga ggagaaggct ggtatcagga agaaggaaag ccagcaagac gattaatggc
       61 tggagatgtc gtcgtaacgc acaaagggat taaacattgg catggtgcga caaaagacag
      121 ttggtttgaa catttggcga tcactgctgg taattcagag tttttagaac ctgtaacaga
      181 tgaagtgtat gaaggattgg aaaatcaata atgtcaagct tgaacagctc ctgaaagtag
      241 attttaatcg aactttcagg agctgctttt ttgattcagt cttttataaa tagaaaatag
      301 ggttaataat aggaatgaga agcacgtcga aagatcttaa tattgataat cggctgggca
      361 tacatgattt ccacgtcatc aatcaaacta cctaacagat gataatcttc ccgatcatgt
      421 cggggattac ctaatagttc ttgatagtag atttccatac tagggtcccg aggattgttc
      481 agcaaatcag caagtacatc taaatcttct ggctgagttt ctgccgtacc ctgtatctct
      541 atgaaaagcc cttcctcagc aaattctact ccaatacgta tttcagttat ttcatgggaa
      601 tataaataag taagaagctc atcgatgatt ctttttgata attctttttc caagtgtctt
      661 tttttccttt ctgacagcgg taaagttatc tagtatcggt acaaggatac ctgcaacgaa
      721 accagttgaa aaaccactgt tatatagatt tagtcctccg tgcaaagtgc tgacgttatg
      781 gacaagagca atatgtagca caccagctat tacaccgtaa aaactgccgt aaaaaccgct
      841 tatgggcgca agacctgtcc caaacacagc tgcaacaagc atgccagtac tggtgatctc
      901 atgaaaagtt ccaaacaaag aagcaataaa tattccgact agtatcggaa aactgttttt
      961 taaatgacaa ccaaaagcac tgaaacccac cgcagataaa atagcaccga tgacaggtcc
     1021 atttaactga ccgccaacca gcaaaacata agaagacagc atcagaccaa ctaagcccat
     1081 gttgatcaaa gtagcaccaa taccagaatt agcaatgaaa tcagttgtca attttccaga
     1141 tgaatcgaaa atttctcgaa tccccgaaag ccgaaaggaa ttataataga aaccaattcc
     1201 gactgtcagc gaaaaaaagc caaaaataat ccaaatgaga ggaaagtgat attcattgaa
     1261 aaccaatgta ttttcgacga ttgaatagct gaataacctc aaaacagcag taaaagtcat
     1321 cgcaatcaac ccagaggtga atccaatatt ataaagtgtg aatccacgat ggaaaactaa
     1381 agtttgagca gctaagggcg gaagaagtat accaattgct atgccagcta aattccccaa
     1441 taaaatacct atcagtaaag gaaaacgcat accaaaagtg atataactaa cgaccggtga
     1501 taatacgcta ccaaacagtc cgatcatgat gtactgagaa aaaggtttat tgacaaatac
     1561 agcgtataga tacacaccta aaatgattga gatagaatta taaaggtttt tgccaaaaaa
     1621 agagaaaccg gtaactgtta gaatcgaagc aaataaggga ccgtttaaaa tcaccttgtg
     1681 ccgataagct aaaagcacag aaagtaaggt caaaatccct acatttatga atgcgcttcc
     1741 gaaaacacct aaagcaaaat aatctgtcaa aagattgctt ggcgaggtaa gaatagttag
     1801 caatccactc caattatcag taggagtatt agaaagcaag ccaataaaaa gaaaaaaaac
     1861 acctgtaaaa ccaagataaa aatactttct tttctgtgaa acgattcgtt tttgaggatg
     1921 aatataaata acgtcagttt cgttttccat tacacacctt ctttttcatt ttctttaact
     1981 ataacttgaa atgaaagatt tggacaacta tcatttaggt caaaactaaa aatgtaaggg
     2041 aacccattga atttaaccaa atttttttaa ttttgcaaaa aaaagttaaa gaagactgaa
     2101 aaaaaattta aaatataacg ttttgagtaa aatgtttgca aaaaatgcta aaaatagggt
     2161 acaataaaac aacatatact gcagtaaaga agtgggggag ttttaatgag aagaaaagtc
     2221 tataccaagg atcaaattct aaaagctgct tatgatgttg ttgcaaagga agggtttaaa
     2281 ggatttactg cgagaaatat tgctaaaaag atggggattt cgacacagcc gatttatctg
     2341 gaattcaaaa acatggaaga tttaaaaaat acgttgctgg attctatctt tgaacaacta
     2401 aaagaagatg ttttccctat tgaacgtact ggtgataaaa tcattgatct cggtttgaac
     2461 tatatctact ttgcaaaaaa gcatcatagt ctttacatgg cattgtacat tgatgaacat
     2521 ggggacggaa agaaaatgca tgatttttca tacaattatt tccgtgaaat agttaaagaa
     2581 gatgaaagat acaatcaatt atcggatgaa tatgtcgatg ctctgcatac tggtacatgg
     2641 gtagcagtga ctggcttagc gtcattgatg tcatcaaaca tcatgcatcc aacagaagaa
     2701 caaatcattg cactgatcaa acggacaatt gactcgattt tagcattaga taatcctgaa
     2761 ataatcagat agtgttctat cagtatgaat ttgatcggta agtccgttgg ctgcaaactg
     2821 tcaatggact tttttgctaa aaaaagcgat ttctgttatt tatctattag aaataggctt
     2881 gacatttcgt gaaaaaagtt taaaaatagt acttacggaa ggttggcaga gtggtaatgc
     2941 accggactcg aaatccggcg aaccggctaa taccggcgcg caggttcaaa tcctgtacct
     3001 tccttatatc aacgattaga ggttgtgaag aagctgtcct gcatcaagta ataagaacgg
     3061 ccaaacaaaa atagcttttc ctattt
//
LOCUS       DAZKLD010000205         3071 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.210, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000205 DAZKLD010000000
VERSION     DAZKLD010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3071)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3071)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3071
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.210"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(291..905)
                     /locus_tag="ACG8KR_002809"
     CDS             complement(291..905)
                     /locus_tag="ACG8KR_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFQ0202112.1"
                     /translation="MSLCYFDYYHQLEEKLRIEEPLMLQAGTWIAKQINQGGRLQIYA
                     SRTLSGVAFEFWDQTPDLVPSILIENPAGGVYETLEGAGQAIIDQLSVKSEDIFLLLS
                     NEGRNPAIIELAQWIKENGHPLIIVTGFDLSRSIKSQHSSGLRLFEFADLVLDNHANL
                     HDAVLTLPNLDPAICGTASLATIFLLQQVLYFTAKQLVQDTSGK"
     gene            complement(1511..1888)
                     /locus_tag="ACG8KR_002810"
     CDS             complement(1511..1888)
                     /locus_tag="ACG8KR_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0202113.1"
                     /translation="MGKLVAVAQSLLDILKWLMEGSNWIFVLPLLLISLFIMFRMVRK
                     KETFLIFIWYAASVGVATLMVELALGSWEACVFALFFAVLYLIIYIPYMKRKSRIGRK
                     GRHTTTFSSTDSLEQSRRTRRRR"
     gene            complement(2188..3069)
                     /gene="galU"
                     /locus_tag="ACG8KR_002811"
     CDS             complement(2188..3069)
                     /gene="galU"
                     /locus_tag="ACG8KR_002811"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFQ0202114.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LDSGIEDILIVTGKEKRPIEDHFDANIELENNLREKEKTELLALVEETTQVNLHFIRQ
                     SHPRGLGDAVLQAKAFIGNEPFVVMLGDDLMKDDIPLTQQLMMDYEETQASAVAVMRV
                     PENETSKYGIIDPKTDSEKGRCRVKGFVEKPEMGKAPSNLAIIGRYLLTPKIFEILET
                     QEPGAGNEIQLTDALQTLNQTEAVYAREFKGKRYDVGDKLGYMKTNIEYGLQHSEIGT
                     SLSAYIIQLSKKLRTES"
BASE COUNT          930 a          642 c          473 g         1026 t
ORIGIN      
        1 agacaagaaa gaatccatat ggattctttc ttgtctcttt ttccactact ttttattttt
       61 ctgacactac atattcttat aactcatttc tcataactgt gaaacaactt ataaaaataa
      121 caaactttaa acaaaacaag cttaaaatca aagatgatcc atcttaaaat aactaaaaca
      181 aaaaaagtag tcgttgttaa tcctcattaa taatcgtttt ctttttttca aacgctccat
      241 ttttatgtgg aacattattt ttttaaggaa ctttgtcaac atgtggataa ctattttcca
      301 cttgtgtcct ggactagctg ttttgctgta aaatacaaaa cttgctgcag caaaaagatc
      361 gttgcaagag aggctgttcc gcagatcgct ggatctagat ttggcaaagt caagactgca
      421 tcatgtaaat tggcgtggtt gtccaaaacc aaatcagcaa attcaaataa tcgcaaacca
      481 ctgctatgtt gcgatttgat acttcgtgac aaatcaaatc cagtaacaat gattaatgga
      541 tgaccatttt ctttgatcca ctgagctagt tcgatgattg ccgggtttct tccttcattc
      601 gaaagaagaa gaaaaatgtc ttctgatttg acagaaagct gatcaataat tgcctgtcct
      661 gcaccttcta gcgtttcata gacacctccg gctggatttt caatcaatat acttggtaca
      721 agatccgggg tttgatccca aaattcaaat gctacaccac ttagtgtgcg gctagcatat
      781 atctgcaatc taccaccttg attaatttgt ttcgcgatcc aagtgccagc ttgaagcatc
      841 agcggctctt ctatccttaa tttctcttcc aattggtgat aatagtcaaa ataacataaa
      901 ctcatttatc tctcccgcct agctatactt gacatgaata gaaacagctg cctaatctaa
      961 ggaagtacat aaaaaaacag tagttagaca gtaacacatg tggtctttgc tgtttcaaac
     1021 acaaaaaagg cattgaacga atacgcccaa taccttgaca tagcaaataa aacaacgtgc
     1081 agaccatgga aaatatctac ataggggtag aattttcacc ctagattagg tacaataaat
     1141 taggtcgatg cactgcattg actatggcaa aatgatcggt cagggtgtat gtgtgtgact
     1201 ggtcattccc tgattcttag cgccaactaa gggtcaggga gccttttttt attcagctgt
     1261 ttctatctga atttattgta agtaatctct tatccaagtg caagcaagaa aatacaaaaa
     1321 aacattggta tgatttatat atatataaat aacataaaaa aaggaatggc ttatcccgat
     1381 acaacaagca ttcctttaaa aaagattctt ttatataagt gaaaaaagct tttttccacc
     1441 aattaaataa atctctcatc tcaatagttt atccattatt ttatcttttt ttgcgtatac
     1501 ctactaaata ttaccgtctt cttctcgttc ttctactttg ttcgagagaa tctgtactag
     1561 aaaaagtagt tgtatgtcta ccttttctgc ctatccgaga ctttcttttc atatatggga
     1621 tataaatgat tagatacagt acagcaaaaa ataaagcaaa tacgcaagct tcccaactac
     1681 ctaacgccaa ttcaaccatc aaggtagcaa ccccaacgga tgctgcatac cagataaaaa
     1741 tcagaaacgt ttcttttttt ctgaccatgc gaaacatgat aaatagacta ataagcaata
     1801 gtggcaacac aaatatccaa ttgcttcctt ccatcagcca cttgagtata tcaagcagcg
     1861 actgtgcaac agcgactaat tttcccatag tgaacacaca ccttttttta taattaaaaa
     1921 attcgctttt ttattctact atgaatcttc tggcttggct ttcatttctc ttatgtaatc
     1981 attttctttt atacaactta aaaaaaaatt aaaccattag cataatattt tctgaatcta
     2041 ttcacgaaag tctttttcta tctttcgttt tccacgactc aactgatcta gagtcaaaaa
     2101 actaaaacga cagttgcgca aatgacgcac aagctgccgc tttaataaca aatagccttt
     2161 cattctcagc aggccactta tttccagtta actttctgtt cttagctttt tactcaactg
     2221 gataatatat gcacttaatg aagtcccaat ttccgaatgt tgaagtccat attcaatatt
     2281 tgtcttcata tatcctaact tatcgcctac gtcgtatctt tttcctttaa actctctcgc
     2341 atagaccgcc tctgtctggt taagagtctg aagtgcatcc gttagctgta tctcgtttcc
     2401 tgctcctggt tcttgagttt ctaatatctc aaatatttta ggagtcagta gataacgtcc
     2461 gataatggct aaattacttg gtgcttttcc catttcaggt ttttctacaa acccttttac
     2521 tctgcaacga cccttttcgc tatctgtttt aggatcaata attccgtatt tagatgtttc
     2581 attttctggt acacgcatga cggcaacagc ggaagcttgt gtttcttcat aatccatcat
     2641 caattgttga gtcaatggga tatcatcttt catcaaatca tcccccagca tgacgacaaa
     2701 cggttcattt cctataaatg cttttgcttg aagcacggca tcccccagtc ctcttggatg
     2761 agattgacgg atgaagtgaa gattgacttg agtcgtttct tcaactaaag cgagtaattc
     2821 tgttttttcc ttttcccgca aattgttttc caattcgata tttgcatcaa agtgatcttc
     2881 aatgggtctt ttttcttttc ccgttacgat caagatgtct tcgattcccg aatccaatgc
     2941 ttcttccaca ataaactgga tcgttggttt atccacgatt ggaagcattt cttttgccat
     3001 ggcttttgtt gccggcaaaa aacgtgttcc tagcccagcg gcaggaatga ctgctttttt
     3061 gaccttcact t
//
LOCUS       DAZKLD010000206         3064 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.211, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000206 DAZKLD010000000
VERSION     DAZKLD010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3064)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3064)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3064
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.211"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            107..853
                     /gene="truA"
                     /locus_tag="ACG8KR_002812"
     CDS             107..853
                     /gene="truA"
                     /locus_tag="ACG8KR_002812"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HFQ0202115.1"
                     /translation="MVRYKAIIAYDGTNFNGFQKQPNGRTVQEEVEKTLQKMANGKEI
                     TVFGSGRTDAGVHAIGQVIHFDYPEERPLERMRFALDTQSPEDIAVRQVEIVSEDFHA
                     RYLVKEKTYQFRVDIGKPRSPFRRHYASYFPYPLDLEKIQRALPDLLGTHDFTSFCAS
                     GSSIEDKVRTIYEAKMEVNETKDELLFTFRGNGFLYKMIRILVGTLLKIGNGRLPEDS
                     IPDIIAKKDRNAAGPTAHPEGLYLYEVKYE"
     gene            1517..1711
                     /locus_tag="ACG8KR_002813"
                     /pseudo
     CDS             1517..1711
                     /locus_tag="ACG8KR_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1899..2675)
                     /locus_tag="ACG8KR_002814"
     CDS             complement(1899..2675)
                     /locus_tag="ACG8KR_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFQ0202116.1"
                     /translation="MNIHHIRNATAILDYGGKKFLIDPMLAEKGTLPPFISKGPFPSP
                     RKDQMNPLVDLPMPVEDIINGIDAVIVTHLHLDHWDDIAKEVLPKEIKLFVQDENDAN
                     EIRLYGFKNVEVLREDTVFESIQLVKTKGKHGRGEILNYVGNVCGIVFKHSTEKTLYF
                     TGDTVWYEAVKQTIDQYNPEIIVANAGDNHFLVGESLIMGKDDLYELHKAIPDSTIIA
                     VHMEAVNHWGLSREELKSFNKEKGISEKVLVPEDGDNIFI"
     gene            2798..>3064
                     /locus_tag="ACG8KR_002815"
     CDS             2798..>3064
                     /locus_tag="ACG8KR_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HFQ0202117.1"
                     /translation="MLDQTDINIIEELSKNSRITMKELGEKVHLTGPAVSARVTKLEE
                     SGVIENYTVKLNMEKLGFSIHAFLIIITQNLNHKPYLSFIKNHEK"
BASE COUNT         1033 a          514 c          549 g          968 t
ORIGIN      
        1 actaaggtca caggcctctt tcttttatct gaaaagaaag aacgaaaaaa ttttataaaa
       61 aaatcattat tccagataac cttttaggaa caaaggagag aaaaacatgg tacgttacaa
      121 agcaatcatt gcatacgacg gaacaaattt taatggattc caaaaacagc caaatgggcg
      181 tactgttcaa gaagaagtag aaaaaacatt acaaaaaatg gcaaatggaa aagaaatcac
      241 ggtctttggt tcgggaagga cagatgccgg agtacatgcg attgggcaag taatccattt
      301 tgattatcca gaggagcgtc cgttagaacg aatgcgtttc gcgctagata cccagtcacc
      361 agaggatatt gctgtaagac aagtagaaat tgtttcagaa gacttccacg caagatattt
      421 ggtcaaagaa aagacgtatc aatttcgagt ggatataggg aaaccacgca gtccttttcg
      481 acgccattat gcaagttatt ttccttaccc tcttgatttg gaaaaaattc agcgggcgct
      541 tcctgattta ttagggactc acgatttcac ctctttttgt gcatcaggaa gctcgataga
      601 ggataaagta cgtacgatct acgaggctaa gatggaggta aatgaaacaa aagacgaatt
      661 gttgtttaca tttaggggaa atggcttctt gtataagatg atacggatct tagtaggaac
      721 gcttttgaaa attggcaatg gccgattgcc tgaagatagt atcccagata ttattgcgaa
      781 aaaagatcgt aatgcagcag gacctacagc acatcctgaa ggactttatt tgtatgaagt
      841 gaaatatgaa taagaggttg tgacagaagt ggtcagcttc aagaaataag aaggaattta
      901 cgaaaattac ttctcaaaat tttgcaaaat ttcggcttat tttcgaagga gctacttctg
      961 gaacaccgtg aatcagaggt tgtgaaaaag ctgccctgca tcaagtaata agaacaacca
     1021 aacaaaaata gctcgtcata tttttcgttt ggttgggctt attaccgcag atgcaagctt
     1081 ttgaacaccg tttaaggaag aggttgtgaa atcagcgttt tgacctgagt attagagaaa
     1141 caaacggaaa aatagttcct catattttgc cgtttgttga gctaataccg aaggtcagct
     1201 gattgaacac cgtttaagaa agaggtcgtg agcctgtcat ttaactctga gtcacaagta
     1261 acaatttcaa aaaacagctt ctcatctttg atgaatatcg tgacttgtgg acgaggcgtt
     1321 gataagtgaa tcatcttttt gttacctatt ttttctgtaa ccaaaaaacg atggaacgtt
     1381 gttttgattg cccttaatga ggacagtcgg aacaggtttg tcatttttat atttaaatgg
     1441 cacaaatttt tctaattatt tgtcatttag cgctgaaaat ttttgctatt tatctaaaaa
     1501 agtgataact acttaagatg aagcagaata tttacgccat acggcataca accgcgaaaa
     1561 atataaaaaa aagaaaacga ttgaacgcgt ttttgcagtc gcaaaggaaa agcatggaat
     1621 gcgctacatc aagtatcgag acttggaaaa caatcaggtg tatacgctga ttgtttttgc
     1681 tgcaatgaat ctgaaaaagt tgtccacttg gcagttggaa catatgtctt catatctttt
     1741 attatacaaa aatagcaaaa gcaccagtct acactcttag taaagaggaa actgatcctt
     1801 ttgtctacat tcttaaagta cttaaactaa gtactttatt ttactcttta ggattgatac
     1861 agtagcatct atccctttat taataatctt ttattttttt aaataaagat gttgtctcca
     1921 tcttcaggca ctaaaacttt ttcggaaatt cctttttctt tattgaaaga ctttaattct
     1981 tctcttgaca gtccccaatg gtttacagct tccatatgaa cggcaataat tgtggaatct
     2041 ggtattgctt tatgcaattc atataaatcg tctttcccca taataagaga ttctccaact
     2101 aaaaaatgat tatctccagc atttgctact atgatttctg gattatactg atcaatagtt
     2161 tgtttaacag cttcatacca aactgtatct cctgtaaagt atagtgtctt ctcagtcgaa
     2221 tgtttgaaaa caataccgca aacattacct acataattca agatttctcc tctgccatgt
     2281 ttgccttttg ttttaaccaa ttgtatagat tcaaaaactg tgtcttctcg tagtacctcc
     2341 acatttttaa aaccatatag tcgaatttca ttcgcatcat tttcatcttg cacaaataat
     2401 tttatttctt taggtaggac ctctttcgct atatcgtccc aatgatctag atgtaaatgt
     2461 gttacaataa cagcatcaat accgttgata atatcttcga ctggcattgg caaatcaact
     2521 agaggattca tttgatcttt tcttggtgat ggaaaaggac ctttcgaaat aaatggtgga
     2581 agtgttcctt tttccgctaa cattggatca attaaaaatt ttttacctcc atagtctaag
     2641 atagctgttg cattacgaat gtgatgtata ttcataaacg ttgtctcctt tttgttgata
     2701 tgaaatattt ttagtataat tcaattccat acaataaata aaccgaacaa aaaaagtctt
     2761 ttactatttt tacttaattt atgaaaggtt gtttaccatg ttagaccaaa cagatataaa
     2821 tatcattgag gaactatcaa aaaatagtcg aatcacaatg aaagaacttg gagaaaaagt
     2881 acatttgact ggacccgctg tctcggcgag agtgacaaaa ctagaggaaa gtggagttat
     2941 tgaaaattat acagtaaaat taaatatgga aaagctaggg ttttcgattc acgctttttt
     3001 aattataatt acgcaaaatt taaatcataa accctattta tcatttatta aaaatcatga
     3061 aaaa
//
LOCUS       DAZKLD010000207         3054 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.212, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000207 DAZKLD010000000
VERSION     DAZKLD010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3054)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3054)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3054
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.212"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            181..948
                     /locus_tag="ACG8KR_002816"
     CDS             181..948
                     /locus_tag="ACG8KR_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0202118.1"
                     /translation="MEKMIEVKHLSKSYGSKLSPVKALDNISFTVEEGEFVGIMGPSG
                     AGKTTLMNILSTINLPTMGSVSIQGKEITKMKNSELSDFRRKKLGFIFQEFNLIDTLN
                     AKDNILLPLAVERMTKEEMEKRVRHVAQLLNIEELLKRYPDELSVGQRQRIAAARALV
                     VQPQVIFADEPTGSLDSKSATELLNYLKTMNQKEKATILLVTHDPYTASYCDRILFIK
                     DGVIFSEVVRRGTRREFFEKVIDMQATIGGGGKMNAV"
     gene            938..2980
                     /locus_tag="ACG8KR_002817"
     CDS             938..2980
                     /locus_tag="ACG8KR_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0202119.1"
                     /translation="MLYKLAVKSFFRQSRGYLVYFFSLTLSVMIYYSFSAMTYDQSLV
                     RRASQDTSIDGGLSFGGFIITVVLLFFVFSANRFFLNRRQKEIGIYQLFGMNKLQISG
                     IYVLEIMIIGLFACISGILLGIIFSKLFSMILVRMMDMDLTSPFFISIPSVVDTLFAF
                     FIILLAVSVYSIWKIWRYPMIRSFGEKEQADSSTMRFRTRHRLLAVIGLLLLTSGYFG
                     ASHFREITSWMISYHYLDTLVFFFPFLIFFVCVVGTYLFFCYTFRLFLIILSKWKIYY
                     YGINFLIMGNTQVHLLKSWRTNSLITVILGISLMTIGGTASISTILSYQEKVSSPMDY
                     QFDVETEKKIKPILAEEGQKITKEITLHYKVIGSIYSQNLEEPMFQTANLITETQYQA
                     FRKVNPDLPNISLKSPGHTVLLDQMQTLFRNISLYGSSIGLPDNQELKIQQMLPSVLG
                     DETMVYLSPVLIVDDTVFEKAKGVDYQVVNVDVSGGNNEKIDERLSNDLSIKWLNAVY
                     YSYDIVNGSLQGEISTKKLPDDQMKEAQFSQESSRLNFSSSYSKLRAVNRRIGINIFV
                     TLFVGMIVIIATGSILTVRQLAEAEEEKENYHLLTKLGIPQRRIRRMIFEQNALTFFP
                     PMILGITHAVFAINVFTQYIKTADYWLAYLVSGLLILIYLLLYVITSQLYCRIIEE"
BASE COUNT          985 a          487 c          616 g          966 t
ORIGIN      
        1 aggtgaaaac ttgtagctgg tggatttgtt ttttgcatat ttaattttgc tgaataaaat
       61 aattgaataa aattttttat tcagtccaag taaaagtgac aaaaacataa gagcaaatta
      121 ttcgtagata tgctactatg caaataaata gaaatgaaaa atgaaagagg aggagaagaa
      181 ttggaaaaga tgatcgaagt gaaacatcta agtaaaagct acggcagcaa actttctccc
      241 gtcaaagcct tagataatat ctcattcaca gtagaagaag gcgagtttgt tgggattatg
      301 ggcccaagtg gtgcaggaaa gacaacgctg atgaatatct tgtccacgat caatttgcct
      361 acgatgggca gtgtctcgat ccaaggaaaa gaaatcacaa aaatgaaaaa cagtgagctc
      421 agtgactttc gtcgaaaaaa actaggattc atttttcaag agttcaattt gatcgatacc
      481 ctaaatgcaa aagacaatat cctattgccc ctagcagttg aacggatgac taaagaggaa
      541 atggaaaaga gagtaagaca tgtagctcaa ttgttgaata tagaagaact gctcaaacgg
      601 tatcccgatg aactttctgt cggacagaga caacgaattg cagctgctag agcacttgtt
      661 gttcagccac aagtgatttt tgcagatgaa ccaactggct ctttggactc gaagtcggct
      721 acagaattgt tgaactactt gaagaccatg aatcaaaaag aaaaagcaac gatactgtta
      781 gtcacccatg atccatatac agcaagctac tgcgatagaa ttttatttat taaagatggt
      841 gtgatttttt ctgaagttgt cagaagaggg acaagaagag agttctttga gaaagtaata
      901 gacatgcagg cgacgattgg cggaggagga aaaatgaatg ctgtataaac tagccgttaa
      961 aagttttttt cggcagtcac gaggctatct tgtttatttt ttcagtttga ctttatccgt
     1021 aatgatctat tattccttca gcgcaatgac ctacgatcaa tctttagtca gaagagcaag
     1081 tcaagatact tcgatagatg gtggattgag ttttggtgga tttatcatta ctgttgtttt
     1141 gctttttttc gtattcagtg ccaaccgatt tttcttgaat agaaggcaaa aggaaatagg
     1201 catctatcag ctttttggta tgaataaact gcagatatca ggtatatatg tgttagaaat
     1261 catgataatt ggattgttcg cctgtatttc tgggatatta ctcggtatta ttttttccaa
     1321 actcttttca atgattttgg ttcgaatgat ggatatggat cttactagtc cattttttat
     1381 ctccattcca tcagtagtag atactttatt tgcttttttc atcatccttt tagcagtatc
     1441 tgtatattca atatggaaaa tatggcgcta tccaatgatt cgaagttttg gagaaaaaga
     1501 acaagcagat agttcaacaa tgcgcttcag gacaaggcac cgattactcg cagtcatcgg
     1561 gttactactt ttaacgagcg gatattttgg agctagtcat tttagggaaa tcacttcatg
     1621 gatgatcagt tatcattatc ttgatacatt agttttcttt tttccttttt taattttttt
     1681 cgtttgtgtg gtcggtactt atttgttttt ctgttataca tttcggttat ttttgatcat
     1741 attgagtaaa tggaagattt attactatgg tatcaatttt ttgataatgg gtaacacgca
     1801 agttcatttg ctcaaaagtt ggcgtacaaa ttctctgatt acagtcattc tcggaatttc
     1861 tttaatgaca attggcggca cggctagtat ctctactatt ttatcctacc aggagaaagt
     1921 ttcctccccg atggactatc agtttgatgt tgagacagag aaaaaaatca agccgattct
     1981 agcagaggaa gggcagaaga taactaaaga aatcaccttg cattataaag tcattgggag
     2041 tatctatagt caaaatctcg aagaacctat gtttcaaact gctaatctga tcacggaaac
     2101 acaatatcaa gccttcagaa aagttaatcc agatttgccg aacatttctt tgaaatcacc
     2161 tggtcatact gttttgcttg atcaaatgca aactcttttt cggaatattt cattatatgg
     2221 atctagtatt ggactgccag ataatcaaga attgaagatt caacaaatgc ttccaagtgt
     2281 attaggtgac gaaacaatgg tatatctaag tcctgtactg attgtagatg ataccgtttt
     2341 tgaaaaagca aaaggtgtgg attatcaagt agtcaatgta gatgtatcag gtggaaataa
     2401 tgaaaaaata gatgaacgac tgagcaatga cctgtcgata aagtggctca atgctgttta
     2461 ttattcttat gatatcgtaa atggaagtct acaaggagaa attagcacaa agaaacttcc
     2521 ggatgaccaa atgaaggaag cacaatttag ccaagaaagc tctcgtttga atttctcttc
     2581 cagctattcg aaattgcgtg cagttaatcg gcgaatcggt atcaatattt ttgtcacact
     2641 atttgtcggg atgatcgtga ttattgctac tggtagtatt ttgacagtcc ggcaattagc
     2701 agaagcagag gaagaaaaag aaaattatca cctattgact aagctgggga ttccacaaag
     2761 aaggatcaga cggatgatct tcgagcaaaa cgcacttacc ttctttccac ccatgatatt
     2821 agggatcact cacgcagtat ttgcaatcaa tgtcttcact cagtatataa aaactgccga
     2881 ttactggttg gcttatcttg tgagtggctt attgatcctt atctatcttt tgctttatgt
     2941 cattacgagt cagttatatt gtcggattat cgaggaatga tatacggaat aagacaagat
     3001 aataaataga actaaatttt gaataccatt tatttggaat aagaggttgt gaca
//
LOCUS       DAZKLD010000208         3045 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.213, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000208 DAZKLD010000000
VERSION     DAZKLD010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3045)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3045)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3045
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.213"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(159..1469)
                     /locus_tag="ACG8KR_002818"
     CDS             complement(159..1469)
                     /locus_tag="ACG8KR_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004650 - polygalacturonase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 28 protein"
                     /protein_id="HFQ0202120.1"
                     /translation="MRTKKGIINALNFKTNDTQQDDTIILQKAIDQGAKEHLPVFIPK
                     GIYLVGALFLKDKSHLIFEEGAVLKGRTDIEAFPEIDTRVAGVEMKWPAAILNVLSAK
                     DILIEGKGIIDGQGDHWWELYWGKDQKSGTRAEYDQKGLRWIADYAIKRPRACLLYHA
                     EHVVVRDLIFQKSGFWNLQITYSNDVLVEKVIIRNNDGPSTDGIDIDSSTNVRVYECD
                     LACGDDCIAIKSGRDGNGARVNRKSSRIEVARCKIRSGYGVTIGSEVSAGVSDVYIHD
                     IDFFQSDCGIRMKSSKERGGVIENIRVENLNMIDVQFPFSWIMDWHNEYNRKNSNDLA
                     QMPESWQAVAEEIPESKQMTKVKDIHIKNVQACLSPGYSLPSRAFDLVAFPEKPIEDV
                     CFSHCQITAKEFGRIEAVQNLCFKSCDLSIETGNTAANNTFDNR"
     gene            complement(1481..2899)
                     /locus_tag="ACG8KR_002819"
     CDS             complement(1481..2899)
                     /locus_tag="ACG8KR_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFQ0202121.1"
                     /translation="MLSQNAVTANTIKKEKKEFGISDKIGYMFGDLGNCFILGLVNSF
                     LMIYYTNVLGVSGAIVGTLFLISRILDAFADVMVGRLCDVSELTETGRFIPWIRKMKY
                     PFCLVTVILFLPFVQHLPMTVKIAYIFVSYIVYGIFLSTINIPYGAMAAAISSNPNDR
                     TSLSTFRSIGSAIGGSTTGFFIPILMYTHNKSGQQVVSGNHFFWISIGCAAIAFVAYM
                     LTCKLTTERVRVVKNEKVSASQLVKGLLANRALIILVIVDIFIVINQILAGTNMTYLF
                     NDYFHNKQAMSVALLFTYGTVVVLAPFATKLTKLFGKKEASVFALLASSCMYLLMYFL
                     HITNAWVYLILLFIATLGSGLFNLMVWAFITDVIDYHQYKTGLREDGTVYGVNSFARK
                     VGQACAGAVGGFMLALIGYQSSTTGGAIQSVLVQERIYTMANLLPAFCLFVSAIILLV
                     GYPLNRSKTVFIGEELKKINER"
BASE COUNT          962 a          625 c          470 g          988 t
ORIGIN      
        1 acgacttagt tatcttatca cgtctaaaaa catgtgtcaa ctcttttttg gaattttcat
       61 aaaatgtttc aatgaagcgc gacaacgttg tttaatatat cattaaaaaa aataaaggtc
      121 aagctaaaat attatttaag cttgacctta ttataatttc aacgattgtc aaaagtattg
      181 tttgcagctg tgttgccagt ttctatagat aaatcacagc ttttgaaaca taagttttgt
      241 actgcttcga ttcgcccgaa ttcttttgca gttatttggc aatgagaaaa gcagacatct
      301 tctataggct tttcaggaaa tgctactaaa tcaaaagctc gggaaggaag tgagtaacct
      361 ggagaaaggc atgcctgtac gtttttgatg tgtatgtctt tgactttcgt catttgcttt
      421 gattcaggta tttcttcagc cactgcttgc caactttctg gcatttgagc aaggtcattg
      481 ctattttttc tattgtattc attatgccag tccattatcc aagaaaaagg aaattgtaca
      541 tcaatcatat tcagattttc taccctgata ttttcgatca ctccccctct ttcttttgaa
      601 gatttcattc gaataccaca gtctgactga aaaaaatcga tatcgtgaat atatacgtca
      661 ctaactccag cactaacttc gctaccaata gttacaccgt atcctgaacg gattttacat
      721 ctagcaacct cgattcttga tgattttcga ttgactcttg caccattgcc atctctgcca
      781 gacttaatag caatacaatc atcaccacag gccaaatcac attcatagac acgcacattt
      841 gttgaagaat caatatcaat cccatctgta cttggtccat cattgttgcg gatgattact
      901 ttttctacta atacatcgtt agaataagtg atttgcaagt tccaaaaacc tgatttctga
      961 aaaatcaaat cacgaacaac aacatgttct gcatgataca gtaaacaagc gcgtggacgt
     1021 ttaatagcat aatccgcaat ccaacgaagt cccttttgat cgtattcagc tcttgtcccg
     1081 cttttttgat cttttcccca atacagctcc caccaatggt ctccttgacc gtcgattatt
     1141 ccttttcctt ctataagaat atctttcgcc gagagaacat tcaaaatcgc tgctggccat
     1201 ttcatttcta cgccggctac tcgtgtatcg atttctggaa atgcttcgat atctgttctc
     1261 cccttgagga ctgcaccttc ttcaaagata agatgactct tatcttttaa aaaaagggct
     1321 ccgactaaat aaatgccttt tggaataaat acaggtaagt gttctttcgc accttgatcg
     1381 attgcttttt gcagtattat tgtatcatct tgttgagtgt cgtttgtttt aaagttcaaa
     1441 gcattgataa tccctttttt tgtccgcaat acgtttctcc ctatctttca ttgatttttt
     1501 tcaattcttc accaataaat acagtttttg aacgattcag tgggtaacct actagcaaaa
     1561 tgattgcgga tacaaataag cagaatgctg gtaacaagtt tgccatcgta taaatccttt
     1621 cttgtactaa aactgattgg atggctccac ctgttgtaga cgattggtaa ccaatgagtg
     1681 ccagcataaa tccgcctacc gctcctgcac atgcttgtcc tacttttctc gcaaaagaat
     1741 ttactccata tactgttcca tcttctctta agcctgtctt atattgatga taatcaatca
     1801 cgtcagtaat gaatgcccag accatcaaat tgaataaacc agatcctaaa gtagcgataa
     1861 ataaaagaat cagatatacc catgcatttg ttatatgaag gaaatacatc agcaagtaca
     1921 tacatgaaga agcaagtaac gcaaatacgc ttgcttcctt tttaccaaat aattttgtca
     1981 gctttgtagc aaatggtgct aaaactacta ctgttccgta agtaaataaa agagcaacag
     2041 acattgcttg tttattatgg aagtaatcat tgaataaata agtcatattc gtcccagcta
     2101 aaatctgatt gatcacaatg aaaatgtcaa caatgactaa aataatcagt gctcgattag
     2161 ctaacaaacc ttttaccaac tgagaagcag atactttttc gtttttgaca actcttactc
     2221 tttcagtagt caatttacaa gttaacatat atgcgacaaa tgctattgca gcacatccga
     2281 ttgaaatcca aaagaaatga ttccctgaaa cgacttgttg tccactcttg ttatgggtat
     2341 acattaaaat cggaatgaag aatcctgtag ttgatccgcc aatcgcagaa ccaatgcttc
     2401 ggaacgtgga caacgaggtg cggtcattag gatttgagct aattgctgct gccattgctc
     2461 catacgggat attgattgtt gagagaaaaa tcccataaac aatgtatgaa acaaaaatat
     2521 aagcaatctt caccgtcatt ggaagatgtt ggacgaatgg caaaaacaaa atgacagtca
     2581 ctaagcaaaa tggatacttc atttttctga tccaaggaat aaatcggcca gtttctgtta
     2641 actctgagac atcacataat cttccaacca tcacatcagc aaacgcatcc aaaattctcg
     2701 aaatcaagaa taacgtacct acgattgccc ctgaaacgcc aagaacattc gtataataaa
     2761 tcatcaagaa actattgact aaacctaaaa taaaacagtt acctagatca ccgaacatgt
     2821 agcctatctt atcggaaatc ccaaattcct ttttttcttt ttttattgta tttgctgtaa
     2881 cagcattttg tgaaagcatt tgaattcctc ctacctcttc tagtaaacgt tttcaaaatt
     2941 ataactcttt ttttcttgtt tttatattca caatgttggc tttttattgc attatcttga
     3001 cagataaaaa acaaaaaata atctaaaaac attgatgcat aagat
//
LOCUS       DAZKLD010000209         2998 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.214, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000209 DAZKLD010000000
VERSION     DAZKLD010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2998)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2998)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2998
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.214"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..586)
                     /locus_tag="ACG8KR_002820"
     CDS             complement(<1..586)
                     /locus_tag="ACG8KR_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0202122.1"
                     /translation="MIERLKDFMISVWDVAMLADESNYTSSIYRLEDSNWIEISLELK
                     TLPNSVNYIIRDITSEAQQLVNYMHGHESIPFSLILLKRSKNISNTRDRFISIITACE
                     IGVKEFYQQEKPDLTLIFENLQSPPVDKLLGSMFKGYFNIEFPKEIRKEIKNFIEKRN
                     KLVHSPQGSKPTIEECFECYMAVLKTFNFFYKIND"
     gene            complement(1224..1481)
                     /locus_tag="ACG8KR_002821"
     CDS             complement(1224..1481)
                     /locus_tag="ACG8KR_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HFQ0202123.1"
                     /translation="MIKAWSDDAWDDYLYWHEQGNKSNIKKINKLIKDIDRSPFAGLG
                     KPEPLKHDLSGKWSRRITDEHRLIYRVENETIFIYSAKDHY"
     gene            complement(1474..1743)
                     /locus_tag="ACG8KR_002822"
     CDS             complement(1474..1743)
                     /locus_tag="ACG8KR_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFQ0202124.1"
                     /translation="MEAVAYSNFRQNLRSYMKQVNEDAETLIVTSKDVEDTVVVLSKR
                     DYDSMQETLRTLSNNYVMEKIRRGDEQFSKGAFKTHDLIEVESDD"
     gene            complement(1874..2053)
                     /locus_tag="ACG8KR_002823"
     CDS             complement(1874..2053)
                     /locus_tag="ACG8KR_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0202125.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESRIMCIALDLL
                     IAQEKAGFEVPALKK"
     gene            2151..2387
                     /locus_tag="ACG8KR_002824"
     CDS             2151..2387
                     /locus_tag="ACG8KR_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0202126.1"
                     /translation="MKQNTSIAKNTTKFKCTAAAWRISISPTNRTNNRGMILFIWISF
                     FLLGYRVLPAYFAPPKRRGRLERLVRNKNHSLSY"
     gene            complement(2434..>2998)
                     /locus_tag="ACG8KR_002825"
     CDS             complement(2434..>2998)
                     /locus_tag="ACG8KR_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0202127.1"
                     /translation="EQLYLESETSQTKVTVSHNRLDVYKRRPETVKKGTELLRLETEK
                     YIMNVYLERSELVENFVSYTICRKHYYSESEYYSIFESMELMEEEMEELNSKDATIDD
                     YYLVFTRFLTCGTESIVLMGMDYELAVPEAFLREYVEEVDNPGDDWYTTLTDFLDTYT
                     YDDTEEIQNELDRKRMPYQRFERKKRQ"
BASE COUNT          914 a          562 c          396 g         1126 t
ORIGIN      
        1 tatcattaat tttataaaaa aaattaaatg tttttagcac tgccatataa cattcaaaac
       61 attcttctat agttggttta cttccttgag gagaatgaac caatttatta cgtttttcta
      121 tgaaattttt aatttccttt cttatttctt tcggaaactc aatattaaag taccctttaa
      181 acatgctccc taataatttg tctacagggg gagattgtaa attctcaaat attagagtta
      241 aatcaggttt ctcttgttga taaaattcct ttacccctat ttcacaggct gtaattatcg
      301 aaataaacct atctctagta tttgatatat ttttacttct tttaaggagt ataagagaaa
      361 aggggatact ttcgtgtcca tgcatgtaat taactaattg ttgagcttca gaagttatat
      421 ctctaattat atagttaacg gaattaggaa gtgtctttaa ctctaatgaa atttctatcc
      481 aattactatc ttctaatctg tatatgctcg aagtataatt tgattcgtca gcaagcatcg
      541 ctacatccca aacactgatc ataaaatctt ttaaacgttc aatcatttca gcaatattgc
      601 taaaatcagc taatttttct ttagaataaa aaaaaacatt tatatcagaa tcaataaatt
      661 ctattttttt attactatat cgtcatcatt atcttgacca tttatgttta ttgttccctg
      721 agtggggagt acttctttca aattcaagtt aaatttatat tccattactt ctcctccaat
      781 atataaaaat tacttaactg tatccaaatt taatatgtaa ataaatcatt aatttttagc
      841 taaattctat gagaagttta tttttgttgt ttttgctaat taactaaaac tagtacaaaa
      901 aatttaaaat agattattaa acctattgct tattctattc agtcatatat ataagcgtca
      961 tcctttagta actaacatcc cacactacaa tagtattata acatatctat tcaacttcaa
     1021 attgttgaaa tttaagccat ttatatttac ttttcctcaa ctttagaaat gattttacta
     1081 accttatatc cgtaatacgc gtaacaaaca cattcattaa taacaatcaa cactaagcta
     1141 tattagcttt ttaaatgact agggtaaata aaataaggat caaaaacttt taagtttctg
     1201 accctttcct tacttccgat tggttaatag tgatcttttg cagaataaat aaatatcgtt
     1261 tcattttcaa ctctatatat cagtctatgt tcatctgtaa ttcttctgga ccattttcca
     1321 gataaatcat gctttaatgg ctcaggtttt cctaatccag caaaggggga acgatcgata
     1381 tcttttatta acttgttaat cttttttata ttgcttttgt ttccttgctc atgccaataa
     1441 agataatcat cccaagcatc atcagaccaa gccttaatca tcagattcaa cctcgattaa
     1501 gtcatgtgtt ttaaatgcac ctttggagaa ttgttcatct cctcgacgaa ttttttccat
     1561 gacgtaatta ttagaaagtg ttctcaacgt ttcttgcata gaatcataat ctctttttga
     1621 taatacaaca actgtatctt ctacatcttt acttgttaca ataagtgttt cagcatcctc
     1681 attaacttgt ttcatataac tacgtaaatt ttggcggaaa tttgaataag ctactgcttc
     1741 cattcctttc accccttatt tatttctata aaacaattgt acattatatt gtacaattaa
     1801 gcaaatgatt aatattctaa cttcttataa ttaaaagtgc tatcactttt agtttttagg
     1861 ataaaaagtg acgtcacttt ttgagtgctg gtacctcaaa tcctgctttt tcttgggcaa
     1921 tcaataaatc taacgcaata cacataatcc tggattcact tttgccgtac ttctgcttta
     1981 gttgctgcaa ttctttttgt ctcttttcat ccaacgtaac gggtaaccgt ttatttccta
     2041 ttgcaattgg cattagtact ctctcctttc atcgaaataa ccctatgaaa aatatggctg
     2101 caatcatctt gaatagtaga ctcacaaggg ctaatacaaa gcgccaaaag atcaaacaaa
     2161 ataccagtat tgccaaaaat actacgaagt ttaaatgcac agctgcagca tggcgcataa
     2221 gtatttcgcc aacaaatagg actaacaata gaggaatgat tttattcata tggatctcct
     2281 tctttctctt aggttatcgg gtgttgcccg cttatttcgc accacctaag cggcgggggc
     2341 ggttggagcg attggtgcga aataagaacc attcccttag ttactaggtt ttttgtcctt
     2401 atttcacaca ttctcacttt tcggtataaa tgattattgc ctttttttcc tttcaaatcg
     2461 ttgatatggc attcgtttac ggtcaagttc attttggatt tcttctgtat cgtcgtaagt
     2521 ataggtgtct aaaaaatcag ttaaagtcgt gtaccagtcg tcgcctgggt tatctacttc
     2581 ttctacatat tccctcaaaa atgcttcggg tacagcgagt tcataatcca ttcccattaa
     2641 cacgatagat tccgttccac acgtcaaaaa tctagtaaat acaaggtaat aatcgtctat
     2701 cgtcgcatct ttgctgttta gctcttccat ttcttcttcc attaattcca tgctctcaaa
     2761 aattgaataa tattctgatt ctgagtaata gtgtttcctg caaatcgtgt agctgacaaa
     2821 gttttcgacc agctcactcc tttctaggta aacattcatg atgtattttt ctgtttctag
     2881 tcgtagaagc tccgttcctt tttttaccgt ttcaggacga cgtttataca cgtctaaacg
     2941 attatggctg actgtgacct tcgtttgact cgtttcactt tccaaataca attgttct
//
LOCUS       DAZKLD010000210         2990 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.215, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000210 DAZKLD010000000
VERSION     DAZKLD010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2990)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2990)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2990
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.215"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1220)
                     /locus_tag="ACG8KR_002826"
     CDS             complement(<1..1220)
                     /locus_tag="ACG8KR_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFQ0202128.1"
                     /translation="MEELKERIVNYVIENSEIKNKWGNNTAQKISEKLNVSRNIVSQY
                     LNEFVKDGEFGKINTRPVIFFSLPEKVPNQQIFSSSEEAEKWYDSSFRVGIERVIGSQ
                     GSLRNVIAQIKAAIYYPPNGLPILLHGETGTGKSYLANYVYKDLLANNYIEEKASFIS
                     VNCSEYANNPELFLSNFFGHVKGAYTGAEDGRKGLAELANNGVLFLDEIHALSNECQE
                     KLFQFMDTRKFHRMGDNENWYTSSCWIIFATSEPPEDKLLSTLLRRIPVKLTLPTLDS
                     RSLYERKQLIKIFFKSEEERLAKKILISQQLLDFLLNYRFSENVGNLKNIIQLTCANA
                     FYFLDDLEELYIQMYHLPQWVNFNGMKVMKRQNELKYIQMDDLEKVDEVFNINELIEY
                     LYQTWKKDTDKQQL"
     gene            complement(1284..1643)
                     /locus_tag="ACG8KR_002827"
                     /pseudo
     CDS             complement(1284..1643)
                     /locus_tag="ACG8KR_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit M
                     N-terminal domain-containing protein"
     gene            complement(1821..2141)
                     /locus_tag="ACG8KR_002828"
                     /pseudo
     CDS             complement(1821..2141)
                     /locus_tag="ACG8KR_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328113.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            complement(2257..>2990)
                     /locus_tag="ACG8KR_002829"
     CDS             complement(2257..>2990)
                     /locus_tag="ACG8KR_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0202129.1"
                     /translation="LCSKCNSFFGSEYVEEYIRFAEDNKNFLYNNTSLKNGKSDLTHS
                     IKKMNSLRVAKAIVGMFFSLNGDEDSMDKPFLDSVREYLSNPKSTLFPHEDYKIIMNY
                     YLPIGAPQNSKIKIPNDIVNDGDTHNVIKGFLPLDLSTGRKIRFSEIQYEGIGFTLID
                     LKNSTFEIKEGLDLKNLLCADDKIKKIQLYKVPILTPTVLTMLEMENNINYIDSGSDL
                     LIRWQVITNFLMELKKYSNTQTKFS"
BASE COUNT          928 a          556 c          402 g         1104 t
ORIGIN      
        1 aaaagctgct gcttatcagt gtctttcttc cacgtttgat aaagatactc tataagttcg
       61 ttgatattaa aaacttcgtc tactttttcc aaatcatcca tttgaatata ttttaattca
      121 ttctgtcttt tcataacctt catgccgttg aaattcaccc attgtggtaa atgatacatc
      181 tgaatataaa gttcttccaa atcatctaag aaataaaagg cattggcaca ggtcaattga
      241 atgatatttt tcaaattgcc aacattttca ctaaagcgat agtttaataa aaaatctaat
      301 aattgttgag atatcagtat tttctttgct agtctttctt cttctgattt aaaaaatatc
      361 ttgattaact gttttctttc ataaagagaa cgactgtcta atgttggaag tgttagcttt
      421 acaggtattc ttctcaacaa agttgataat agtttatcct caggtggttc tgatgttgca
      481 aatataatcc agcaacttga tgtgtaccag ttttcattat cacccatccg atgaaatttc
      541 ctcgtatcca taaactgaaa aagtttctcc tgacattcat tgctcaatgc atgaatttca
      601 tctaaaaata agactccatt attagctaat tctgctaaac cctttcttcc atcttcggct
      661 cctgtatatg caccttttac atgcccaaaa aaattggaca agaaaagttc tggattattg
      721 gcatattcac tacaattaac tgaaatgaaa gatgccttct cttctatata attattggcc
      781 aacaaatcct tatacacata atttgctaaa tagctttttc cagttccagt ctcgccatga
      841 agtaaaatgg gtaagccatt tggcggatag tagattgctg ctttaatttg agcaattaca
      901 tttcgcagac ttccttgact tccgataacc ctttctattc caaccctaaa agagctatca
      961 taccattttt ccgcttcctc tgaagagcta aaaatttgtt gatttggaac tttctccggt
     1021 aaagaaaaga agattactgg acgagtattg atttttccaa attctccatc ttttacgaat
     1081 tcattcaaat attgagagac aatattacga ctaacattta atttttcaga tattttctgt
     1141 gctgtattgt tcccccattt atttttaatc tcagagttct ctatgacata attgacaatg
     1201 cgttctttta attcttccat cacagcacct cctcgacact attcgaattt tgccgacagt
     1261 atttttccat aaatatgact tgctaaaaag gctttgttta gatcattcaa ttggaacgtt
     1321 gcttgttttg cttgatccgc taagacgttg aaaagatact ctggcgaaat cgcatacccc
     1381 aaaatatcaa cgattgttgc aatcagatct tcttttgatt cttcatctga aagtaattca
     1441 cgatacaaca tcgtttgttt gcttactgtg tcatattcct ctaaggattc atctgctaaa
     1501 agaaccactt gttccaacaa acggtcggat aaatatttat agaaaatcag ccccaataaa
     1561 taatttttat attctgacgc atccatttta ctacggagat tatctgccgc actaaataac
     1621 ttctgattta attctgcacc caaaaagata cactcctatc tcttatttaa agttgctata
     1681 taccctctta ttttagcata ctcagtttta gaaaaagcaa aaaaataaag aaacttattc
     1741 ttgataacaa gctacgtgac gtatatccct aacacaaaaa tagccatatc ctttggtttg
     1801 gatacggcta tttgatatta ctcattcaat tatcaaatca cagtaggtgt gaatctcatc
     1861 gggatcggca atatctactt ctgattagga tcaaaggtga ttttctcaat aaaggtgtga
     1921 tacaaagctt taacttgctt cttctcactc tggataagaa agcaatccaa actagtcaat
     1981 atctgttgga catctttgac ttgaatcggt tccccttgtt gttgaaagat ccctaagatt
     2041 tcattctctc gaatttgtaa ctgtcttcgt ttttcagtca attcatttaa acgatctttt
     2101 aacatcggaa acaattcggg actatcttcc aaagcagctt cctgtttttg ctctaatagt
     2161 tagattttgc gtaccatttt cttcttttat tttccttcga ttgctgattt taatatagtc
     2221 taaaggaaat ttatatttat tgtattcttt ataacttcaa gagaacttcg tttgagtatt
     2281 actgtatttt tttaattcca taagaaaatt agttattacc tgccacctaa tcagcaaatc
     2341 tgaacctgag tcaatataat taatattatt ttccatctcc aacattgtaa gtactgttgg
     2401 tgttaatata gggactttgt acaattgtat ttttttgatt ttatcatctg cacaaaggag
     2461 attttttaaa tctaatcctt cttttatttc aaaagttgaa ttttttaaat ctataagagt
     2521 aaaaccaatt ccttcatact gaatttctga aaatcgaatt tttctcccag ttgatagatc
     2581 aaggggcaga aaacctttta ttacattgtg agtgtcacca tcatttacaa tgtcattagg
     2641 aattttgatt ttagaattct gtggagcacc tattggaaga taataattca ttataatttt
     2701 gtagtcttca tgaggaaata aagtactttt aggattgcta agatattctc gtaccgaatc
     2761 taagaatggc ttatccattg aatcttcatc cccatttaaa ctaaaaaaca ttcctacaat
     2821 tgcttttgca actctaagtg aattcatttt ctttattgaa tgtgttaaat cagacttacc
     2881 attctttaaa cttgtattgt tataaaggaa atttttattg tcttctgcaa atctaatata
     2941 ttcctcaaca tactcactac caaaaaaact attacattta ctacataacg
//
LOCUS       DAZKLD010000211         2957 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.216, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000211 DAZKLD010000000
VERSION     DAZKLD010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2957)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2957)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2957
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.216"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            113..1009
                     /locus_tag="ACG8KR_002830"
     CDS             113..1009
                     /locus_tag="ACG8KR_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HFQ0202130.1"
                     /translation="MIQYYYTKKEWGVVMEKEKLKILEELRRILNNKNEAIIILNNYF
                     KGGVGKSKLSTMFAYLTDKLNLKVLMIDKDLQATLTKDLAKTFEVELPRVNFYEGLKN
                     GNLASSIVHLTDNLDLIPGTFDLMLLPKLTRSWTFENESRLLATLLAPLKSDYDLIII
                     DTVPTPSVYTNNAIVASDYVMIPLQAEEESTNNIQNYISYLIDLQEQFNPGLDMIGFV
                     PYLVDTDSATIKSNLEELYKQHKEDNLVFQNIIKRSNKVSTWSKNGITEHKGYDKKVL
                     SMYENVFFEMLERIIQLENEKE"
     gene            1101..1316
                     /locus_tag="ACG8KR_002831"
     CDS             1101..1316
                     /locus_tag="ACG8KR_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002401482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HFQ0202131.1"
                     /translation="MIVGNLGAQKAKRNDTPISAKKDIMGDKTVRVRADLHHIIKIET
                     AKNGGNVKEVMDQALEEYIRKYLPDKL"
     gene            1334..1606
                     /locus_tag="ACG8KR_002832"
     CDS             1334..1606
                     /locus_tag="ACG8KR_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HFQ0202132.1"
                     /translation="MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELDTKNTRLLEV
                     NLLNQLEVAQEVDLFQQPFEELQAIHEYWRSMNQYSKQILTKEKVA"
     gene            1608..2471
                     /locus_tag="ACG8KR_002833"
     CDS             1608..2471
                     /locus_tag="ACG8KR_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HFQ0202133.1"
                     /translation="MANITDFTEKQFEDRLEKNVERLTKNRLAVESPTAFLLGGQPGS
                     GKTSLRSAISEETQGNVVIIDNDTFKQQHPNFDELVKLYEKDVVKYVTPYSNRMTEAI
                     ISRLRDKGYNLVIEGTGRTTDVPIQTATMLQAKDYETKMYVMAVPKINSYLGTIERYE
                     TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETP
                     SISPKETLERELNRKVSGKEIQPTLERIEQKMVQNQHQETPEFKAIQQKMESLQPPTP
                     PIPKTPKLPGI"
BASE COUNT         1167 a          468 c          516 g          806 t
ORIGIN      
        1 gttcaacttt tggggttcag ttcataaaga gggcttttat atattaatca caaatcactt
       61 atcacaaatc acaagtgatt aatcacaaat cacttgtgat ttgtgattct taatgataca
      121 atattactat acaaaaaaag aatggggcgt agttatggag aaggaaaaac taaaaatact
      181 tgaagaatta agacgtattt taaacaataa aaatgaagca attattatct tgaataatta
      241 ctttaaaggt ggtgttggaa agtccaaatt atcgactatg tttgcttact tgacagacaa
      301 attgaattta aaagttttaa tgatcgataa ggacttacag gcaacattga caaaagactt
      361 agcaaaaact tttgaggtag aattgccacg tgtcaatttt tatgaaggct tgaaaaatgg
      421 aaacttggct tcttctattg ttcatttgac tgataattta gacttgatcc ctggcacgtt
      481 tgatttgatg ttactgccaa aattaactcg ctcatggact tttgaaaatg aaagtagatt
      541 gcttgctact cttttagcac ctttaaaaag tgactatgat ctcattatta ttgatactgt
      601 accaacgcca agcgtttata caaataatgc aatcgtggcg agtgattacg ttatgatccc
      661 tttacaagca gaagaagaaa gtacaaacaa cattcaaaac tatatttcct atttgattga
      721 tttacaagaa caatttaacc ctggactaga tatgatcggt tttgttcctt atttagttga
      781 tacggacagc gcaacgataa aatcaaacct ggaagaactg tacaagcaac ataaagaaga
      841 taacttggtt ttccaaaata ttatcaagcg aagtaataaa gtaagtacct ggtctaaaaa
      901 tggaattaca gaacacaaag gctatgacaa aaaagtttta tccatgtatg agaacgtatt
      961 ttttgaaatg cttgagcgaa ttattcaatt agaaaacgaa aaagaataga atcacaaatc
     1021 acaagtgatt aatcacaaat cacttgtgat ttgtgattgt tgatgataaa ataagaataa
     1081 gaagaaatag aaagaagtga gtgattgtgg gaaatttagg cgcacaaaaa gcaaaacgaa
     1141 atgatacacc aatcagtgca aaaaaagata taatgggaga taagacggtt cgtgttcgtg
     1201 ctgacttaca ccatattata aaaattgaaa cagcaaaaaa tggcggaaac gtaaaagaag
     1261 ttatggatca agccttagaa gaatatatac ggaaatattt acctgataaa ctttaaaaag
     1321 gagtgaaaat gaaatggcag ttacgtatga aaaaacattt gaaatagaga tcattaacga
     1381 attatcggca agcgtttata atcgagtatt aaactatgtt ttgaaccatg aactagatac
     1441 taaaaatact cgtttactag aagtgaatct tttaaatcaa ttagaagtgg cacaagaagt
     1501 tgatttattt caacaaccat ttgaagaatt acaagctatt catgagtatt ggcggtcaat
     1561 gaatcaatat tcaaaacaaa ttttgacaaa agagaaagtg gcttaacatg gcgaatatta
     1621 ctgacttcac cgaaaagcaa tttgaagatc gtttagaaaa gaatgttgaa cgactaacta
     1681 aaaatagact agcggttgaa tcgccaaccg cttttttact tggtgggcaa ccaggatcag
     1741 gtaaaactag tttgcgatcg gcaatttccg aagagacaca aggaaatgtt gttatcattg
     1801 ataatgatac gttcaaacaa cagcacccta attttgatga actagtgaaa ctttatgaaa
     1861 aagacgtagt aaaatacgtt accccttatt ctaatcgcat gacagaagcg atcataagcc
     1921 gtttgagaga taaagggtat aatttagtga ttgaaggtac aggacgaaca acagacgttc
     1981 ctattcaaac cgcaacaatg cttcaagcca aagattatga aacaaaaatg tatgtcatgg
     2041 cagtacctaa aatcaactca tatttaggaa caattgaacg gtatgaaact atgtatgcag
     2101 atgatccaat gacagccagg gcaacaccaa aacaagcgca tgatattgtt gtcaaaaact
     2161 taccgaccaa tttagaaacc cttcataaaa cgggcttatt tagcgatata aggctttaca
     2221 atagagaagg agtgaaactc tattcaagct tagaaacgcc ttccattagt ccgaaagaga
     2281 ccttagaaag agaattaaat cgtaaagtat cagggaaaga aattcaaccg actttggaga
     2341 gaatagagca aaaaatggtt caaaatcaac accaagaaac ccctgaattt aaagcaattc
     2401 aacaaaaaat ggaaagcttg cagccaccta caccaccaat acccaaaaca cctaaacttc
     2461 cagggattta aaccttaaaa aagtaaaaga gtagttacca aaaaacggta actactcttt
     2521 ttttataaaa acatttcctc attttttaaa agctgatcga gaaataaagc acacaactgt
     2581 attctttctt ttttcagttg gtacattttg cagtctgtaa cgtgccattt actgtttgtt
     2641 accttaacat atccagtcga aaacacaagg gtatataaac gagcaagctc gcccttgtgt
     2701 tttctttgtg tctatgttgc gttctcgtca cagcaaacga cacgaacaca ctggcgaaaa
     2761 tgaaccaaca aaacgaaaaa agaaaggagc gtacactgta atttatttct agcttttaaa
     2821 acatgaggaa aacagcttca cttcctcaaa cgcctaacaa aaatggttgt caaaaaacta
     2881 aaaatcaaaa aaagaaaaga ggaaaagaaa atggaaaaaa caaaaaaatt acaattagaa
     2941 gattttacag aaaatgg
//
LOCUS       DAZKLD010000212         2894 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.217, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000212 DAZKLD010000000
VERSION     DAZKLD010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2894)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2894)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2894
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.217"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..227
                     /locus_tag="ACG8KR_002834"
     CDS             <1..227
                     /locus_tag="ACG8KR_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0202134.1"
                     /translation="KTTEFDILHALMSTPNKVFSRSDLIEKVKGIEFDGLDRSIDSHI
                     KNLRHKIENDAKSPMYILTVHGTGYRFGGH"
     gene            227..1620
                     /locus_tag="ACG8KR_002835"
                     /pseudo
     CDS             227..1620
                     /locus_tag="ACG8KR_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294791.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
     gene            1635..1841
                     /locus_tag="ACG8KR_002836"
     CDS             1635..1841
                     /locus_tag="ACG8KR_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0202135.1"
                     /translation="MFQKQIKIAKLVCECWAKKIFFQLISIDSVEKVHLDILSNSVVI
                     FSKKDLSIATIKKEVFGLQIYEMT"
     gene            1862..2458
                     /locus_tag="ACG8KR_002837"
     CDS             1862..2458
                     /locus_tag="ACG8KR_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdhK family protein"
                     /protein_id="HFQ0202136.1"
                     /translation="MKTVKKMTKLVFASLGIIVFLGACSNQSESNNSNSTNEESTSTA
                     SSEMNSMEGMNHEGMVPSSMKDAANPKFPVGSNVILLGDHMEGMKGANAQVVGAFDTT
                     MYEVSYEPKTGGPMVNNHRWVVQEELKDTETVANEGDTVILNADHMDGMMGAEAKVDK
                     SITGTVYVVNYTPTDGQEEVKNHMWVTEDEMEYDENNE"
BASE COUNT         1050 a          396 c          573 g          875 t
ORIGIN      
        1 ttaaaacaac tgaatttgat attctacacg ctctaatgtc cacacctaat aaagtctttt
       61 ctcgtagtga tttgatagag aaagttaagg gaatagaatt tgatggacta gataggagca
      121 tcgattctca tataaaaaac ttaagacata aaattgagaa tgatgctaaa tcacctatgt
      181 atattttgac agtccatggt acaggataca gatttggtgg ccactaatga gaaaaagaac
      241 gataaaaact caattagctg tttccttttt agcaatcgca acattaataa taggatcgat
      301 tagtttagtc gcattaagtt tgatgaacaa tcattttagt aaatacgtgg aagagcggca
      361 agaagatttg ttgaatcagt atgtatatac aattgattta ttgtggttaa atagtgggga
      421 aacatggaat agtgaagaac ttgcagcact atctgagaaa gtattagaga ataatattta
      481 tttttctata gaagacgaac aaggcaacat ggtttgggaa cttactggaa aagatttgaa
      541 gtcagcacag gagaaactga aaaaaatgca ttgaaagttt ctgagaaaaa cagtgtgaaa
      601 ctagacgaaa caatagaagt gaaaaagaag ttaattaatg atggaaacga gtttggaaaa
      661 gtaacgtttt attatttcgg accttttgct tatacagagc acgatgcctt gtttatctcc
      721 agtatgaaac aaagtttaat gtatgttgca attgcagctc ttcttgtatc attcatactt
      781 gcttcgtgga tctctgctag attaggattg ccactaaagc atgtaagcga ttttacacat
      841 aaattaactc gtggggaata tgctgataaa atcccacaag aaacttcaat tattgaaata
      901 aattccttaa ttgattccct aaatgattta agtaatcagt tggagaaaca gcatgggctt
      961 cgaaaaagat taactaccga tatttcacat gaattaagaa ctccattagc tacgcttaaa
     1021 ggcaatgtgg aaggaatgat tgacggagtc tggaaaataa ctcccgagcg cctacaatct
     1081 tgttatgacg aaatagatcg tctaactcgt ttaattggaa atatagaaat aatcaacaaa
     1141 attgaagcaa aatatgatca tttaaataaa actgagttta acatatataa gttgattgaa
     1201 tccgttattg aaaatttcgc cagcaaaatt gagtcaaaaa accttcatgt tgaaatccaa
     1261 ggggataaca tcaatatatc tgcagataaa gataaaatga accaagttgt tatcaatctt
     1321 ttgaataatg ctattaaatt tacgcaaaag gagggaacca ttaagttttc tataagtaaa
     1381 aataaagacc atgttctttt aattgttgaa gacaacggca tcggtataga aaaagatcag
     1441 caacttcata tttttgatag attttatatg gctgacccct ccagaagtag agctttaggg
     1501 gggcaaggta tcggattagc tattgtaaag agcgttattg aagcgcataa aggtagtata
     1561 actgtaaaaa gtaaattagg tctaggaact aaatttgtta taaagttacc atttcaataa
     1621 aggaggagag ttatttgttt caaaaacaga taaaaattgc taaactagta tgtgaatgtt
     1681 gggcaaaaaa aatatttttt caactgattt cgatagatag tgtcgaaaaa gttcatttag
     1741 atatcttatc aaattctgta gtaatctttt ctaagaagga cttatcaata gctacaatta
     1801 aaaaagaagt ttttggatta caaatttatg agatgaccta aatgtgatta attaaaggag
     1861 gatgaagaca gtgaaaaaga tgacaaaatt agtatttgca agtcttggaa ttattgtatt
     1921 tttaggagcg tgtagcaatc aaagtgaatc gaataattct aatagcacaa acgaggaatc
     1981 gacttctaca gcgtctagtg aaatgaatag tatggaggga atgaatcacg aaggtatggt
     2041 tccttcttca atgaaagatg ctgcgaaccc taaattcccg gtgggaagta atgttatact
     2101 gctaggcgat catatggaag ggatgaaagg tgcaaatgct caagttgtag gggcctttga
     2161 tactacaatg tatgaagtaa gctatgagcc aaaaactggg ggaccaatgg ttaacaatca
     2221 tcgatgggta gtacaagaag agttaaaaga tactgagact gtagcaaatg agggcgatac
     2281 tgttatcttg aatgccgatc acatggatgg gatgatggga gctgaagcaa aagtagataa
     2341 atctattaca ggaactgttt acgttgttaa ttacactcca accgatggtc aagaagaagt
     2401 taagaatcat atgtgggtaa ctgaagatga gatggaatat gacgaaaata atgagtaatt
     2461 ttatatacta aaccaacaag ctggtacatg gtcttcgtat tctcaacatg aggaatatga
     2521 gctctttgtt aataaggact gatgagttgt gcaaaatcaa atctgggtca gaacgaacct
     2581 cattctggct cagatttttg gctcttcgac atttagtgct ggtagagaga caactgaaaa
     2641 tttagtcata accagttagg cagagtgctt aactggtttt tgttcgcatt tattattctt
     2701 ttgatattgg ctgcttgccc ttcaatttta tttggtttct taatcaactc ttcatataac
     2761 cccaaatcat atattaatga ggtgcctcct attcctataa atctatctct tctttaatag
     2821 tatgagaaaa gtctactctt gtagatttaa attgtcaaca aaaaatttac agatgtaaat
     2881 gataagttga atag
//
LOCUS       DAZKLD010000213         2828 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.218, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000213 DAZKLD010000000
VERSION     DAZKLD010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2828)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2828)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2828
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.218"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            614..1915
                     /locus_tag="ACG8KR_002838"
     CDS             614..1915
                     /locus_tag="ACG8KR_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFQ0202137.1"
                     /translation="MPDLHHIKLLLGIKDKNIFITNVESKSIKNIKSLVVSATLSKEI
                     RRCPLCKQMNHEGMIVKNGKKKSLIQLNKCANQLTYLALAKQRYHCRGCHTYFTANTY
                     IVDRNCFIAKQVRYKILEELTEKQAMTTIAKHCGVSWSTVSRTLASLIPMTKVKRNWL
                     PRCLLMDEFRSLKNQVGPYSFSCMDGDTGKLLDILPSRKKKALVSYFMQFERRARLKV
                     KVIVTDMNVSYASLIKECFPKAKLVVDRFHIVKHLIRKFEDIRVRIMKSFDRNDPTQA
                     KYYRQLKALSRLLIKRQDKLVYDKWTKWRNFGWAYLTESEVVDRLLSISDELRIAYFY
                     YQEILQAFHDKEADTFFKLVRTMPNSVPKELHSIKKAFINYESGIRLALELPYSNAKI
                     ENLHTHIKALKRVAYGFRSFRKMKTRIFLLNNLITYESKNI"
     gene            complement(2019..2219)
                     /locus_tag="ACG8KR_002839"
     CDS             complement(2019..2219)
                     /locus_tag="ACG8KR_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFQ0202138.1"
                     /translation="MNNGTVKWFNADKGFGFITGEDGNDVFAHFSAIQGDGFKTLEEG
                     QAVSYDVEDGQRGPQATNIVKA"
     gene            2615..2764
                     /locus_tag="ACG8KR_002840"
     CDS             2615..2764
                     /locus_tag="ACG8KR_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0202139.1"
                     /translation="MTFYNKDIQYRVTLDTDLNLFVVFDKENTNHVATGVTIEQAVQE
                     LKKSA"
BASE COUNT          919 a          542 c          463 g          904 t
ORIGIN      
        1 atcactctac aataacggcg aaagcacaaa aaataaaaaa attattacgt tattaaaaaa
       61 acagatgaaa gatcaggttt tattagatct cattctttca tcacttgaag ggatttaatg
      121 aactactttt tcctctttgt cgtttttata tattcgtatt ccttcaaaca gtaggatagc
      181 ctctttcaat gcttgatatt tgaactgtcc gttacttaaa aatttagatg tgccgccctc
      241 ttcctagaac atattctttt ttactttttc attaattgac agaccaatcg aacgaaactg
      301 taaatttcga taggataatg attggtcaaa ctcattcatt ttcaagacat ctcgattcat
      361 attcaagata ggaatctttt tatcaacatc actttattcg tattttcctc taccataaaa
      421 gcttcacgct ccacaaaatt gttttataca ctactattca gcccattcaa cgaccaatga
      481 attttttcgg ctctataatt tatgggactg atagaaaatc attctatcgg tcccttttga
      541 gcatgaaaaa acaaaacata cagaagctcc gttagaattg aatcaccaca acacaatcaa
      601 aggagcgttc tgtatgcctg atcttcatca tatcaaacta ctacttggaa tcaaagataa
      661 aaacattttc atcacaaatg tggaatcgaa gagcattaag aatatcaaga gtcttgttgt
      721 ttctgcgacg ttaagtaaag aaattcgtcg ctgtcctttg tgtaaacaga tgaatcatga
      781 aggcatgatc gttaagaacg gaaagaaaaa atcgctcatt caacttaata aatgtgcgaa
      841 ccaactgact tatcttgctt tagcgaaaca acgctaccac tgtcgtggct gccacaccta
      901 ttttacggcg aatacctaca ttgtcgatag aaactgcttt atcgctaaac aagtgcgcta
      961 taaaatttta gaagagctaa ctgaaaaaca agcaatgacg acaattgcta agcattgcgg
     1021 cgtttcttgg tcgaccgttt ctcggacact cgcttctctg atccccatga caaaagtaaa
     1081 aagaaattgg ttaccgcgtt gtttattaat ggatgaattt cgttcactga aaaatcaagt
     1141 cggtccctac tccttttctt gtatggatgg cgatactgga aaattactgg atattcttcc
     1201 ttcacggaag aaaaaggctc tcgtttctta ttttatgcaa tttgaacgac gcgcacgatt
     1261 gaaggtaaag gtcatcgtga cggacatgaa tgtttcttat gcctctctga taaaggaatg
     1321 ttttccaaag gcaaaactcg ttgtggatcg ttttcacatt gtcaaacatc tcattcgtaa
     1381 atttgaggat attcgcgtac gaatcatgaa aagctttgat cgaaacgatc caacacaagc
     1441 caaatattat cgacaattaa aggcgttgtc tcggcttctg atcaaacgac aggacaagct
     1501 tgtttatgac aaatggacca aatggcgcaa ttttggttgg gcgtatctca ccgaaagtga
     1561 agtggtggat cgtctgcttt cgatctctga cgaattacga atcgcctatt tttactatca
     1621 agaaattctt caagcgtttc acgacaaaga ggcggacact ttctttaaac ttgtaagaac
     1681 gatgcccaat tccgttccaa aagaactgca ttctataaaa aaagctttta taaactatga
     1741 atccggcatt cgtttagccc tcgaattgcc ttattcaaat gccaaaatag aaaatctgca
     1801 tacccacatc aaagcattga aacgagtcgc ttatggcttt cgaagctttc ggaaaatgaa
     1861 gaccagaatt ttcttactaa ataatctgat tacctatgaa tcaaaaaaca tctaaagtag
     1921 aaaaggaatt ctaccttaga tgttcttatc tcactctatc agtcctattt gacaaagagc
     1981 ccttttcttt cgaaaagacg gttaaaagtc aatttttatt aagctttaac aatgtttgtt
     2041 gcttgagggc cacgttgacc atcttcaaca tcgtaagaca ctgcttggcc ttcttctaaa
     2101 gttttgaagc cgtcaccttg gatagctgaa aaatgtgcaa atacatcatt tccatcttca
     2161 ccagtgataa agccaaaacc tttgtctgcg ttgaaccatt ttactgtacc gttattcata
     2221 aataaattcc tcctgatacg tatcaatgta cgttttttcg ttgcaataaa tatttgatag
     2281 gcaaaaggag ttttatctat gtagatatat cgctcaaatt acgtttcaaa atagattact
     2341 catttaatat atcatgcttt ttctaataac acaaggataa tgcaatgaaa tataaaattc
     2401 attgtcttct attgtcttcg aatcgaagaa aaagttgaat aaccgcgatt gattcacttt
     2461 ttgttttctt gtttatttgc ttttatctgc aacttttgac tttaaaattt cttaactatg
     2521 aaaacctttc aattggtggt atacttataa atggaatttc gttatcaacg cgttcgttgc
     2581 ctttcagcaa gtatttaaag aaaggagaat ggctatgaca ttctataaca aagatataca
     2641 atatcgagta accttggata cagatttgaa tctatttgtc gtttttgaca aagaaaatac
     2701 caatcatgta gcgacaggtg ttacgatcga acaagcagtg caagaattaa aaaaatctgc
     2761 ataaggagtg gaaaccctca tatggacgat tctcgttctt ctctttaaac gcatctactg
     2821 gtagataa
//
LOCUS       DAZKLD010000214         2797 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.219, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000214 DAZKLD010000000
VERSION     DAZKLD010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2797)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2797)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2797
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.219"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            234..1559
                     /locus_tag="ACG8KR_002841"
     CDS             234..1559
                     /locus_tag="ACG8KR_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HFQ0202140.1"
                     /translation="MMDYSKEPVNDYFLIDMKSFYASVECIERNLDPLTTELVVMSRA
                     DNTGSGLILASSPAAKKKYGISNVSRPRDLPQPFPKTLHVVPPRMNLYIKRNMQVNNI
                     FRRYVADEDLLIYSIDESILKVTRSMNLFTTEGTRSQRRKKLAQMIQENIKEELGLLA
                     TVGVGDNPLLAKLALDNEAKHNDGFLAEWTYQNVPDKVWNIPEMTDLWGIGSRMKKRL
                     NQIGIMSIKDLANWNPYTIKSHLGVIGLQLYFHANGIDRTDIAIPPEPTKEKSYGNSQ
                     VLPRDYTRRNEIELVVKEMAEQVAIRIRQHNCKTGCVHLYIGTSILETRPGFSHQMKI
                     PITDNTKELQNYCLFLFDKYYEGQEVRHVGITYSKLVYTDSLQLDLFSDPQKQIDEEN
                     LDKIIDKIRQKYGFTSIVHASSMLEGARSITRSTLVGGHAGGAGGLKND"
     gene            1552..1902
                     /locus_tag="ACG8KR_002842"
     CDS             1552..1902
                     /locus_tag="ACG8KR_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0202141.1"
                     /translation="MIDYNDRGRLKWAGFYLSDHTEKIDADNEQRANQNLAKEQMTTE
                     EVAEVLNAAVIKNRSVCIQKEERNAEGYYPEDIVSKILGSDELGIYVGKNKVHYDEIR
                     HIEFYQDNKWGRLE"
     gene            1899..2069
                     /locus_tag="ACG8KR_002843"
     CDS             1899..2069
                     /locus_tag="ACG8KR_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0202142.1"
                     /translation="MNIIQQYELKYITFDQLSEEIWGYGQRLINEVGVERFSFYVEAA
                     AGYHKYEYYIGY"
BASE COUNT          986 a          442 c          539 g          830 t
ORIGIN      
        1 tttttttcta atataactaa gcaaaactgg ataatacgac acttattttc aaattttcac
       61 aagactaata gtaaatatcc atttaataaa gtgatacaat gtgttgattt tacaaatcaa
      121 cagaagaagt agggaaataa cttacctatg cttctttttt tgtacattta atcgattctt
      181 ctttacaaaa acgaacgtat gtttgtataa tgtggaagaa tggagtgtta tttatgatgg
      241 attattcaaa agagccagta aatgattact ttttgatcga tatgaaaagc ttttacgcaa
      301 gtgtcgaatg tatagaaagg aatttagatc cattaacgac tgaattagtc gtcatgagtc
      361 gagcggataa tacaggatca gggttgattt tagcttcttc accagctgca aagaagaagt
      421 acggtatttc taatgtcagt cgaccacgag atctaccgca accttttcct aaaacgttac
      481 atgtcgttcc accacgcatg aatctctata ttaagcgaaa tatgcaggta aacaatattt
      541 tcagaaggta tgtagcagac gaagatttat tgatttactc gatcgatgaa tcgatcttaa
      601 aagtgaccag atcaatgaat ctttttacga ctgaaggaac aagaagtcaa cggagaaaga
      661 agctggctca aatgatccag gagaatatta aagaagagct aggattgctt gctacagtag
      721 gtgtcggaga taatccctta ttagctaaac ttgctttaga caatgaagca aaacataatg
      781 atggattcct tgctgaatgg acgtatcaaa atgttcctga taaagtatgg aatattcctg
      841 aaatgacaga cctttggggt attggatctc gaatgaaaaa aagactcaac caaattggta
      901 tcatgagtat taaagactta gctaattgga atccatacac tattaagagc catttaggtg
      961 tgattggctt acagctgtat tttcatgcga acgggatcga tcgaacagat atagcgatcc
     1021 caccagaacc gacaaaggaa aaatcatatg gtaattcaca agtgttacca cgagattata
     1081 ccaggagaaa tgaaatcgaa ctggtggtaa aagaaatggc ggaacaagta gctattcgta
     1141 tcaggcaaca taactgtaaa acaggttgtg tccatttata cattggcacg tcgatcttag
     1201 aaacgagacc aggtttttca caccagatga agatccctat aacagataat acaaaggaat
     1261 tgcagaatta ctgtcttttc ttgtttgata agtattatga aggacaagaa gtaaggcacg
     1321 ttggaataac ctatagcaag cttgtgtaca ctgattcgct tcagctggat ttatttagtg
     1381 atccacaaaa gcaaattgac gaagaaaatt tagataaaat tattgacaag attcggcaaa
     1441 aatatggttt tacgtcgatt gtacatgctt cttctatgtt agaaggcgct cgtagtatta
     1501 ctagaagtac attggttggt ggccatgcag gcggcgcagg agggcttaaa aatgattgat
     1561 tataatgatc gtggacgttt aaagtgggca ggtttttatt tatctgatca tactgaaaaa
     1621 attgatgccg acaatgaaca acgagccaat caaaatttgg caaaagaaca aatgacgact
     1681 gaagaggttg cggaagtatt gaacgctgca gtcataaaaa atagatcagt atgtattcaa
     1741 aaagaagaac gaaacgctga aggatactat ccagaagata ttgttagcaa gatcttaggt
     1801 agtgatgagc tggggatcta cgttggaaaa aacaaagttc actacgacga aatacgtcat
     1861 atcgaatttt accaggataa taaatggggt cgattagaat gaacattatc cagcaatatg
     1921 agctaaagta tataaccttt gatcagctat cagaagagat ttggggttat ggccagcgtt
     1981 tgataaatga ggttggtgtt gaacgcttct ctttctatgt agaagctgca gcaggatacc
     2041 ataaatacga atactatatt ggctattaaa gtgcaataaa attatatttc ttttatgttt
     2101 tacattttgt atcaattctg ataaaattac ctataggtaa ttaaaaaata taaaaaaact
     2161 agttacaacc ttaggacgtt gtaactagct tgaagcatac tagagctaac taaaatagca
     2221 ctagaaagca atgttaaacc cgtccaacaa acttgattta acattgagac cagcttattc
     2281 agctaaatat agttgaataa gcttcttttg ttttaaaaaa acaaaaggtg tactacgaat
     2341 aacgcaagta aaagaaatac tattacaacc agtaggctac aagtaagtat tactaaacat
     2401 tgcgtagtta gaacctttgc tctattgtct aaagccacaa aattagagat cagctttatg
     2461 atgctaagag cgagctgcat ccatgcggac ataggttctt ttgacactat taattctcct
     2521 ttttaggata agggaaattg ctagtagttt tttcattagc accatccccc ttcatttgat
     2581 tgagtctagt gctgctgtga tagtttgctg tttaatatgc ttacattctt attataacat
     2641 aaatgaaaca aacacatggt tagaggtcgt atcaatcaaa atagtgtctc acaaacgatt
     2701 tattacttac tcctgctgac taacggactg ttcttcagta actgtaacta tatgtttgtt
     2761 aaaaccacca ttacctgtag ataaaaacca atatatt
//
LOCUS       DAZKLD010000215         2658 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.220, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000215 DAZKLD010000000
VERSION     DAZKLD010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2658)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2658)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2658
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.220"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            272..>2658
                     /gene="esp"
                     /locus_tag="ACG8KR_002844"
     CDS             272..>2658
                     /gene="esp"
                     /locus_tag="ACG8KR_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repetitive surface protein Esp"
                     /protein_id="HFQ0202143.1"
                     /translation="MVSKNNKRVFLEKTKKRVLKYSIKKLSVGVASVLIGVGLVLGTT
                     ELVKAQDEISPSSPLETAISSVQVGDKVASGNTFQENPGYTKNYNFSDLQFSPQELTG
                     DTLKGNTIGFEVYGKHNIAASTKNWEIRLQLDERLAKYVEKIQVDPKKGIGSSRRTFV
                     RINDSLGRPTNIWKVNYIRASDGLFAGAETTDTQTAPNGVITFEKSLDEIFKEIGIDN
                     LKTDRLMYRIYLVSHQDDDKIVPGIDSTGYFLTDSDDFYNSLDVSENNPDQFKHGSVS
                     AKYEKPNTQTKDGSGSTGANGAIILDHKLTKNYNFSYSSSAKGTPWYANYKIDERLVP
                     YVAGIQMHMVQADKVTYDVSFESGTKVADLAIERREGHENYGIGSITNNDLTKLIDFA
                     NASPRPVVIRYVLQLTKPLDEILEDMKATAQVEENKPFGEDFIFDSWLSDTNKKLIQN
                     TYGTGYYYLQDIDGDGNPDDKEESGDTNPYIGKPELEEVYDVDTTVKGKVFIHELAGT
                     GHKAQLVDKEGTVLAEKTIAPNEKDGAPISDTVEFEFTGVDSSKLIAKDELKIQIVSP
                     GFDKPEEGSTVIKESPKAVDKQTVVVGFKPDAKESIRNNKNLPEDAEYSWKTEPDTSN
                     VTDSTKGIVTVKIGNRTFDVDVEFAVKASQAMENDATYVPITTTPETTVQSGKPTFDK
                     PDVPLAKDAFSISDVYNKYFGNASVDANTGIVTFTPAKGVGESEPITGTIPIKIVYQD
                     GSVGTTDLAVTVSKDIYENPGENIPAGYHKVTFTAGEGTSIESGTTVFAVKDGVS"
BASE COUNT          969 a          409 c          533 g          747 t
ORIGIN      
        1 aaaacatgct tcatcgcata gaaatgaact taatttcaga aagaatacat acatatcttt
       61 ttatatgttt tcagctgctg aatggttaaa aagcacaaat atccgaaaaa aacatgaaga
      121 aatgtacttt ttttgttgta atgttcatga aattctctaa gtaaattagt taacataagg
      181 ttagtaagat tcctattctg tgaataatac gtatcagatt tatcttttat tcttactagg
      241 aaagtaatta ttaagggagt tgatttgaat aatggttagc aagaataata agagagtatt
      301 tctagaaaaa acaaagaaac gagtattgaa gtacagtatc aaaaaattaa gtgttggggt
      361 tgcatctgtt ttgattggtg ttggtcttgt cttgggaaca actgaactag ttaaagcaca
      421 agatgaaatt tctccaagca gcccacttga aacagcgata agctccgtac aagtaggtga
      481 caaagtagct tcggggaata cttttcagga gaatcctggt tatacgaaaa attataactt
      541 ttcggattta caatttagtc ctcaagaact aactggagac acactaaaag gcaatacaat
      601 tggatttgaa gtttacggaa aacataatat tgcagcatca actaaaaact gggaaatccg
      661 tcttcaatta gatgaacgat tagctaaata tgtagaaaaa attcaggtcg atccaaagaa
      721 aggaattgga tcttctagac ggacctttgt aagaattaat gattcgctgg gtagacctac
      781 aaatatttgg aaggtcaatt atattcgtgc aagtgatgga ttatttgctg gggcagaaac
      841 aaccgatacg caaaccgctc ctaatggggt aattacgttt gaaaagagcc tagatgaaat
      901 ttttaaagag ataggcatag acaatctcaa gaccgatcgt ttaatgtatc gaatctactt
      961 ggtaagtcat caagacgatg ataaaattgt accgggtatt gatagtacgg gctacttttt
     1021 aacagattca gatgatttct ataatagctt agatgtatct gaaaataatc ctgaccagtt
     1081 taaacatgga tcagtaagtg caaaatatga gaaacctaat actcaaacaa aagatggttc
     1141 aggatccaca ggtgctaatg gagctataat cttagatcat aagttaacga aaaattataa
     1201 tttctcatat tcatcttccg caaagggcac gccttggtat gctaattaca aaattgatga
     1261 acgattagtt ccatatgtag cgggtataca gatgcacatg gttcaggctg acaaagtaac
     1321 atatgatgtt tcgtttgaat ccggaacaaa agtagctgat ttagcaatcg agcgacggga
     1381 aggtcatgaa aattatggca taggttcaat aactaataat gatttaacta aacttatcga
     1441 ttttgctaat gcaagtccac gtccagtcgt tattcgatat gttttacaat tgactaagcc
     1501 attagatgaa atcttagaag atatgaaagc aacagcacaa gttgaagaaa ataaaccatt
     1561 tggtgaagat ttcatctttg attcttggtt gtcggatacg aataaaaaat taatccagaa
     1621 cacttatgga acaggttatt attatttgca agatattgat ggtgatggaa accctgacga
     1681 taaagaagag agcggagaca cgaatccata tatcgggaaa cctgaattag aagaagtata
     1741 tgatgttgac acaacagtta aggggaaagt attcatccac gagttagcgg gaacaggtca
     1801 caaagcccaa cttgttgata aagaaggtac tgtattagca gaaaaaacta tcgctccaaa
     1861 tgaaaaagat ggggctccaa tttcagatac tgtagaattt gaatttacgg gtgtagattc
     1921 aagtaaatta atcgcgaaag atgaattaaa aatccaaatc gtttctccag gttttgataa
     1981 accagaagaa ggttcaaccg ttattaagga atcaccaaaa gcggttgata aacaaaccgt
     2041 ggtagttgga tttaaaccag atgctaaaga atcaattcgg aataataaaa acttacctga
     2101 agatgcagag tattcatgga aaacagagcc tgatacttct aacgttactg atagtacgaa
     2161 aggtattgta actgttaaga tcggaaatcg aactttcgac gtggatgtag agtttgctgt
     2221 aaaagcttct caagctatgg aaaatgatgc aacatacgta cctataacaa caactccaga
     2281 aacgacagtt caaagtggaa aacctacatt tgataaacca gatgttcctt tagctaaaga
     2341 tgccttttca atttcagatg tctataataa gtacttcggt aatgcaagtg ttgatgcaaa
     2401 cactggtatt gttacattca ctccagctaa aggtgtagga gaatcggagc cgattactgg
     2461 aacaattcct attaaaattg tttaccaaga tggttctgta ggcacgaccg atttagcagt
     2521 aactgtaagt aaagatattt atgaaaatcc aggagaaaac attcctgcag gctaccacaa
     2581 agtaaccttc accgcagggg aaggaacaag tattgaaagt ggaacaacag tctttgcagt
     2641 gaaagacggc gtaagctt
//
LOCUS       DAZKLD010000216         2620 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.221, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000216 DAZKLD010000000
VERSION     DAZKLD010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2620)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2620)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2620
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.221"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            446..1045
                     /locus_tag="ACG8KR_002845"
     CDS             446..1045
                     /locus_tag="ACG8KR_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFQ0202144.1"
                     /translation="MKGVEPIRDKKKIDAMKAILASGKYGQRNLVLFSIGINTAYRIS
                     DLRQLKLRDVLEISRGRVIVKERLAMKEQKTAKHNSVFISNKLRKVILDYVQSEFPEQ
                     LQAQDFSKYLFPSRKGADTPLTRQSLWRIIHEAGTAVGLKEIGPHSMRKTFGYFLYKQ
                     GTKTEIIQSLLNHSSQRETLRYIGITQEDKDTAVKSLDL"
     gene            1094..1810
                     /locus_tag="ACG8KR_002846"
     CDS             1094..1810
                     /locus_tag="ACG8KR_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HFQ0202145.1"
                     /translation="MNEEKQPNFPDKYHLSRKESVYLLKKNIVELVYNAGKFEGLNTT
                     LLQTEGIIKYNRANNVAVDDVLTVVNLKRGFEMLLNDVQEPLLETSKRINRIVAAEDA
                     LFPGEIRTGGVELSTIQGRYVPPMLTEDEVNNQYGEIMNQEISDTEKALRLFLFISKN
                     QIFWNGNVGTALLIANKIMFSNGLGLLSIPENVFMKFNELLSKYCNSNLLVNESNIIS
                     FLYEQCIFGINYLKIIQHTI"
     gene            1824..2603
                     /locus_tag="ACG8KR_002847"
     CDS             1824..2603
                     /locus_tag="ACG8KR_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002396205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="abortive infection family protein"
                     /protein_id="HFQ0202146.1"
                     /translation="MMTIDEAKQLATYFSVGSAGYILDFTNVTWNIFTKKIVGIEIQN
                     HYGGSKGVSFQKFLLDDQFDDSLKNRLIQSLKGIMDRDKKYESTEMNPIVQLLFEEVF
                     KEDTSTALRKMKFSSEIDLLYIRQLPIRIQTDIKNKDFDSVLTKANTLIDEVLKFIIE
                     NTEGPSVNINLFKSKELRKEVFGRLNIKTEKDMDNRIKALVGALNTLSDKILEMRNSQ
                     GDAHAHGSNRIVIKEEEAILVANSSMVLCEYVFTKYKGLNR"
BASE COUNT          955 a          385 c          500 g          780 t
ORIGIN      
        1 aataactaaa agtgtcaaaa caatgatgaa atcatttagt tcttataaat tagacaagag
       61 aaaataaata ttccatatct actttattgg atccataaca tgactaaatt ctgaatactt
      121 tttaaagctt atcatttggt aaaactgaat ataagttaat tatatagact gaaaattttg
      181 aattttcaga atagaataac ttaaaagtac tgacaaattt tttaaagggg caatattaat
      241 gttctgagat aatcaaatct taccgaaaat atcaaaagtc caatgtgaca caattggatt
      301 ttgtcacatt cgttaaaatc gctccatcct tagaggctct aataggaaat cacttttctt
      361 ttagcgcctt ttttctatag cttgtttaaa tgtgacttat tatctgttat aatcgtatta
      421 agtaacattt gagaggtgtc taaaaatgaa aggcgtggaa ccgattcgcg acaaaaaaaa
      481 gatcgatgcc atgaaggcca ttttggcttc cggaaaatat gggcaacgaa atttggtgct
      541 tttttcgatc gggatcaata cagcctatcg aatctccgat ttaagacagt taaaattaag
      601 ggatgtgcta gaaatttccc gtgggcgggt gatcgtcaaa gaacgtctgg ccatgaagga
      661 acaaaaaacg gccaaacaca attcggtctt catttcgaac aaattgcgaa aagtgattct
      721 tgattatgtt caatccgaat ttcctgagca gttgcaagca caagacttca gcaagtattt
      781 gtttccaagt cgaaaaggag cagatacgcc tttgacccga caaagccttt ggcggatcat
      841 tcatgaagca ggaactgcag ttgggttaaa agaaattggt ccccattcga tgcgtaagac
      901 ctttggctat tttttataca agcaaggaac caaaacagag atcattcagt cgctgctaaa
      961 ccattcttca caacgggaga ccttacgcta tattggaatc acccaggaag acaaagatac
     1021 tgcagtaaag agtttggatt tatgacagaa taatgcccta tataatagaa ggaaaaattc
     1081 aagaggtgag aaaatgaacg aagaaaagca gcctaacttt cctgataaat atcacttatc
     1141 tcgcaaagag tctgtgtatt tattgaagaa aaacattgta gaactggtgt ataatgcggg
     1201 gaaatttgaa gggttaaata caacgttgtt acaaacagaa gggattatta agtataatcg
     1261 ggccaacaac gtggctgtag atgatgtcct gaccgtcgtt aacttaaaaa gaggctttga
     1321 aatgctttta aatgacgtac aggagcccct gttagagaca agtaagcgga ttaatcggat
     1381 cgttgcagca gaggatgcgc tttttcccgg tgaaatacga accggtgggg tagaattatc
     1441 gacgattcaa ggaagatatg tgccaccaat gttaactgaa gacgaggtaa acaatcaata
     1501 tggtgagata atgaatcaag agatatcaga tacggaaaaa gccctgcgac tgtttctatt
     1561 catttcgaaa aatcaaatat tctggaatgg taatgtgggt acagccttgt tgattgcgaa
     1621 taagattatg tttagcaacg gtttaggact attatcaata cccgaaaatg tatttatgaa
     1681 atttaacgaa cttttatcaa agtattgcaa cagtaatcta ttagtcaatg agtcaaatat
     1741 tatatcattt ttatatgaac agtgtatctt tggtattaat tatttgaaaa taatccaaca
     1801 tacaatttaa gaggtataag taaatgatga ctatagacga agcaaaacaa ttagcaactt
     1861 atttttcagt tggttcagcg ggatatattt tagactttac taatgtaacg tggaacatat
     1921 ttacaaagaa aatcgtaggg attgaaatac aaaatcatta tgggggctca aagggagtat
     1981 cctttcaaaa gtttctgctt gacgatcaat ttgatgattc gttaaagaat cggttaatcc
     2041 aatctttaaa aggtattatg gatcgagata aaaaatatga atctactgaa atgaatccca
     2101 tagttcagtt gctttttgag gaagtattta aagaagatac ttcaactgca ttaagaaaaa
     2161 tgaaattttc aagcgagatt gatttacttt atattcggca gctacccatt aggattcaaa
     2221 cagatattaa aaacaaggat tttgactctg ttttgacaaa agcaaatact ttaatagatg
     2281 aagttctaaa atttattatt gaaaataccg aaggaccgag tgttaatatt aatttattta
     2341 agtcaaaaga attaagaaaa gaagtatttg gccgcctcaa tattaaaact gaaaaagata
     2401 tggataatag aatcaaagcc cttgttggag cactcaatac attatcagat aaaatactcg
     2461 aaatgagaaa tagtcaagga gatgctcatg cacatggctc aaatagaata gtaattaagg
     2521 aagaagaagc aattcttgtt gcaaattctt ctatggtttt gtgcgagtat gtgtttacta
     2581 agtataaagg attaaataga taataaaatg catattttat
//
LOCUS       DAZKLD010000217         2556 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.222, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000217 DAZKLD010000000
VERSION     DAZKLD010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2556)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2556)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2556
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.222"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..399
                     /locus_tag="ACG8KR_002848"
     CDS             <1..399
                     /locus_tag="ACG8KR_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HFQ0202147.1"
                     /translation="ADGLTLDIWLRKKRDTQAAYAFLKRLHKQFGEPKAIVTDKAPSL
                     GSAFRKLQSVGLYTKTEHRTVKYMNNLIEQDHRPIKRRNKFYRSLRTASSTIKGMETI
                     RGIYKKNRRNGTLFGFSVSTEIKVLMGIPA"
     gene            482..1099
                     /locus_tag="ACG8KR_002849"
     CDS             482..1099
                     /locus_tag="ACG8KR_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011834674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0202148.1"
                     /translation="MWIFKQEIFAKLFSLDGLGWGILFFIFLFLGSMWIWFVDKKNSY
                     EKVIVKKMNRNEFDDFSKEHRVELYDSHEINSTSPSYYWVFGCEEKPLEIKLLKMLEN
                     PPVIEDFSAKNVEKQMFEQVQIRGQNKISLEPDEYAAFFLETNSGTSMPVFKVKLKMN
                     GRLCTYDLVANMRYGSTGDRRTILKFEQTFRSFLKNRVSQIFNLD"
     gene            1263..1595
                     /locus_tag="ACG8KR_002850"
     CDS             1263..1595
                     /locus_tag="ACG8KR_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0202149.1"
                     /translation="MNNREILAKEMADLKAKEAKIRAKRKQIRQKEMISLAKLYCKKE
                     GFKTPAEALDFLESNKRNHSVNDEVLVSLRKFADGLVWQENGAYWRTNDVKGLTQFMA
                     QFRSENVN"
     gene            1919..2080
                     /locus_tag="ACG8KR_002851"
     CDS             1919..2080
                     /locus_tag="ACG8KR_002851"
                     /inference="COORDINATES: protein motif:HMM:NF013258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
                     /protein_id="HFQ0202150.1"
                     /translation="MAYMVARTQKHKVGSLNGLQNHVDRKTKNHTNKDIDITKSGSVA
                     KFSKKSILV"
     gene            2112..2556
                     /locus_tag="ACG8KR_002852"
                     /pseudo
     CDS             2112..2556
                     /locus_tag="ACG8KR_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
BASE COUNT          893 a          361 c          524 g          778 t
ORIGIN      
        1 gcggacggct taaccttaga tatctggtta cgaaagaaac gggatacgca agcagcctat
       61 gctttcttaa aacgactcca taaacagttt ggtgagccga aagcaattgt gaccgataaa
      121 gcaccttctc ttggctccgc ctttagaaag ttacagagtg tgggtttata tactaagaca
      181 gagcaccgaa ctgtgaagta tatgaacaat ttaatagaac aagaccatcg acctattaag
      241 cgacggaata aattttatcg aagtctccgg actgcctcat ccacgattaa ggggatggag
      301 accattcgag gaatatataa aaagaaccga agaaatggaa cgctcttcgg cttttcggtg
      361 tctactgaga tcaaggtatt aatgggaata ccagcctaag atatttggag ttcagagagg
      421 gcgcgtttga ttttcaaact tcgcaacaga accattattt taaagtggga ggaaaattgt
      481 tttgtggata tttaaacaag aaatctttgc taaattattt agtttagatg gattaggttg
      541 gggaatctta ttttttattt ttttattttt aggatctatg tggatctggt ttgttgataa
      601 aaaaaattct tatgagaaag taatagtgaa aaaaatgaat cgaaatgaat tcgatgattt
      661 ttcaaaggaa catagagttg aattgtacga tagtcatgaa attaacagta cttctccatc
      721 ttattattgg gtatttggtt gcgaagaaaa accattggag attaaattgt tgaaaatgct
      781 tgaaaatcct ccagtcattg aagatttttc tgcaaaaaac gttgaaaaac agatgtttga
      841 acaggtacaa ataagaggtc aaaacaaaat ttcattagaa ccagatgaat atgcagcctt
      901 ttttttagaa acaaattcag gtacttcaat gcctgttttc aaagttaaat taaaaatgaa
      961 tggtcgttta tgcacttatg atcttgttgc aaatatgcgc tatggttcta ctggtgatag
     1021 acgtactatt ttaaaatttg agcaaacctt tagaagtttt ttgaaaaata gagtatccca
     1081 aatttttaat ttggattgaa aaaattaaaa atttggccaa aaatactaca gttttttaga
     1141 gcaatcatct tatgatgatt gctctttttt tgttgttttt gtgtcattcg ttgttcaaat
     1201 cctctattcg taaaataaat attttaaact agaaatatgc cttattttta ggaggaatca
     1261 aaatgaacaa tagagaaata ttggcaaaag aaatggcgga tttgaaagca aaagaagcga
     1321 agattagagc gaaacgaaaa cagattagac agaaagaaat gatttcttta gcgaaattat
     1381 attgtaaaaa ggaaggcttt aagacacctg ctgaagcatt agattttttg gaatcaaata
     1441 aaagaaatca ttcagtaaat gatgaggttt tagtatcgct aagaaaattt gcagatggat
     1501 tagtttggca agaaaatggt gcatattggc gtacgaatga tgttaagggt ttgactcaat
     1561 ttatggcaca atttcgttca gaaaatgtga actaaattga ggaggggttg aagtgaaaag
     1621 caatactttt cacttttggt tttttgcgga ataaaaattg tgaaaacaat gacgcaaaaa
     1681 gccaatggtt ttgaacgaaa aaaggacgta aaaaaggaat gcgaaggcag tcgtttttcg
     1741 ttcgcttttt cgtttgagag aaacggagtt tctcatcggc tgttaagccg atatagccct
     1801 cggcagagct tacagacttt acgcagtata agtctagtaa gttatacctt acatggaggt
     1861 ttttccgaat ttagcttgtg attctcgcaa taattttaac gaaaggaagt acacagttat
     1921 ggcctatatg gtggcacgaa cacaaaagca taaagttggg tcgttaaatg gacttcaaaa
     1981 tcatgtagat cgtaaaacga aaaatcatac gaataaagac attgatatta cgaaaagtgg
     2041 ttctgttgca aagttttcca aaaaatctat tttagtgtaa aattgagaaa aaagacagag
     2101 aggatagagt aatgaatcat tttaaaggca aacaattcaa aaaatgacgt cattattgtc
     2161 gctgttagtt actacctgcg ttacaatcta agctatcgtg aagttcagga attgttatat
     2221 gatcgtggaa taaatgtttg tcatactacg atttatcgtt gggtgcaaga gtacagcaaa
     2281 gtcctcgatt atctttggaa gaagaaaaat agacaatcct tctattcatg gaaaatggac
     2341 gaaacctata tcaaaattaa gggacattgg cattatcttt atcgtgcaat tgatgcggac
     2401 ggcttaacct tagatatctg gttacgaaag aaacgggata cgcaagcagc ctatgctttc
     2461 ttaaaacgac tccataaaca gtttggtgag ccgaaagcaa ttgtgaccga taaagcacct
     2521 tctcttggct ccgcctttag aaagttacag agtgtg
//
LOCUS       DAZKLD010000218         2534 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.223, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000218 DAZKLD010000000
VERSION     DAZKLD010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2534
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.223"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(256..405)
                     /locus_tag="ACG8KR_002853"
     CDS             complement(256..405)
                     /locus_tag="ACG8KR_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0202151.1"
                     /translation="MTFTKTNMQYRVAFDTTSNRFMAIDAQNENNVAFGITIEQAVRA
                     LNESR"
     gene            867..1079
                     /locus_tag="ACG8KR_002854"
     CDS             867..1079
                     /locus_tag="ACG8KR_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0202152.1"
                     /translation="MVKAEVGRSYICKPIGVSHELIGCIERTYINTAVVYVESYDQRD
                     YQLINESKYRMLVKFCDISAPAELVS"
     gene            complement(1357..>2534)
                     /locus_tag="ACG8KR_002855"
     CDS             complement(1357..>2534)
                     /locus_tag="ACG8KR_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HFQ0202153.1"
                     /translation="SWSLTKSRLLAITGQFIVIGGTILLLSSLGYIVVILAQSMVEQF
                     FPDYALISAVFAMTLLQGILLFNIVMSTVGIFYIIVDFMDDEGFLPEIPKWFIPQAPN
                     LRFSALKNTGLTLFAVFFGIGVCLYNMDYLTSAVQTKPVTVSHRGVSTQNGAQNTLEA
                     LEKTSRDYHPDYVEMDVQETKDGHFVVMHDANLRHLTGVNGTPQDLTLKELTNLTVTE
                     NGVQAPVCSFDSYLEKAHKLNQKLLIEIKTSKKDSDDLVERFVHKYRKVILENHHILQ
                     SLTYQTVSDLKEAEPNFYVGYILPFNIVGPPVTPADFLTMEYSTINRNFINSAHQDGK
                     EVYVWTINDSDAMSRMMFYGVDGIITDEMGLLNESVKQTDEEITYSDKLLNFVLGVG"
BASE COUNT          862 a          437 c          443 g          792 t
ORIGIN      
        1 tttttatttt tattatatta aatattatac agataataac ggaatattat catcgaaaca
       61 gctaacaaaa ttgtcataaa aaacgagatg gttgatatta gaacttgtgt ttttgtttag
      121 gtctagagat aaagagagac aatttccaaa tatatgtaat gaatacaaaa agacgtttgt
      181 cgcggattac cgttacaaac gtcttttatt gattttccga gatctcatca gtcgccattt
      241 ggaatggttc actccttatc tagattcgtt taatgctcgg actgcttgtt cgattgtaat
      301 accaaacgca acgttatttt cgttttgtgc atcaatcgcc ataaaacggt tagaagttgt
      361 atcaaaagct acgcggtatt gcatatttgt ttttgtaaac gtcataaaat tcatctcctt
      421 tgtaatgtaa tttagttctt gaaagtttcc tttaaacttt cgaacttata ccaagtttag
      481 catcgtacgt tttttgatgc aattaaaaag catttatcat tttccttatt tattttttct
      541 taaatggttt agctattttc ttttaatccg ataagtagcc caatcatcaa ggttacaaat
      601 actattgctt gaatataaat catatgctca cctctttata aataacttag caatataaag
      661 aaaaaaacgc acgaacaaca taggaatata aacaaacgtt tttgtcacaa cgtctaagat
      721 cgtgttaaaa aatagttatc taaaaagcag aaagatactc aaaataaaaa ataaaaaaat
      781 ggataaaaac gttttcaaat acaaggtaat ttgttattat aaaataaaga aaaaacaaaa
      841 taaaaataaa gaaaagaggg aatgaaatgg taaaagcaga agtaggaaga tcttatatat
      901 gcaaaccgat cggagtatct catgaactaa tcggctgtat tgaacgtaca tatatcaaca
      961 cggctgtcgt gtatgttgaa agttatgacc aacgagacta tcaacttatc aatgaaagca
     1021 agtatcgcat gctcgtaaaa ttctgtgata ttagcgcacc tgctgaattg gtttcttaaa
     1081 cagtattttt gagaattcgc aagaactccc ttataatata aataaagaca gaagggagcg
     1141 agaagatatg ccaaaaagaa aaagttcagg gaaaatggga attattcttg cagtgatctc
     1201 tgcaattcct gctattttag ctattgttcg tgcgatgaag gaatcaagag aaagttaagc
     1261 acacattatg agcgtcaaac aaaaaacgca tatacctgcg gtggcaggtt atgcgttttt
     1321 tgttgtataa agactttttt cattaacgat ttttgattag ccgactccca aaacaaaatt
     1381 tagcagttta tctgaataag tgatttcttc atctgtttgt ttcacacttt catttagtag
     1441 tcccatctca tcggttatga taccatccac accataaaac atcatgcgac tcatggcatc
     1501 tgaatcgttg attgtccata catatacttc ttttccgtct tggtgggcag aattaatgaa
     1561 gttacggttg atcgtactat attccattgt cagaaaatca gcaggcgtca cagggggccc
     1621 aacaatattg aaaggtaaga tgtagcccac ataaaagttt ggttctgctt cttttaaatc
     1681 tgaaacggtt tggtacgtca aactttgcaa aatatgatgg ttctctaaga taactttgcg
     1741 gtatttatgg acaaatcgtt cgacaaggtc atccgaatct tttttagagg tcttgatctc
     1801 aataagtaat ttttggttta gcttatgtgc tttttccaaa taagaatcaa aagaacaaac
     1861 aggggcttgc actccgtttt cagtaactgt aagatttgtc aattctttca atgtaagatc
     1921 ctgtggtgtt ccatttaccc ctgttaagtg acgaagattt gcatcatgca tcactacgaa
     1981 atgaccatct tttgtttcct gaacatccat ctctacataa tcaggatgat agtctctgct
     2041 tgttttttca agtgcttcta aagtattttg cgccccattt tgggtactca cacctctgtg
     2101 agagacagtc actggttttg tctgaacagc tgaagtcaga taatccatat tgtaaagaca
     2161 gacgccaata ccgaaaaaaa ctgcaaaaag agtcaaacca gtgtttttca gcgcagagaa
     2221 acgcaagttg ggggcttgtg ggataaacca cttagggatc tcaggcaaga agccttcgtc
     2281 atccatgaaa tcaacaatga tataaaaaat ccctacggta gacatcacga tattgaataa
     2341 taaaatccct tgtaaaagcg tcatagcaaa cacagcgctg atcaatgcat agtctgggaa
     2401 aaattgttca accatacttt gtgccaaaat aacaacgata tatcctaaag aagaaaggag
     2461 taaaatcgta ccgccaatca caataaattg gcccgtgatg gccaaaagac gcgatttggt
     2521 caatgaccag cttt
//
LOCUS       DAZKLD010000219         2475 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.224, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000219 DAZKLD010000000
VERSION     DAZKLD010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2475)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2475)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2475
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.224"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            581..1540
                     /locus_tag="ACG8KR_002856"
     CDS             581..1540
                     /locus_tag="ACG8KR_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFQ0202154.1"
                     /translation="MTYTHLTPTELVMIEAYFNQSHSVSKVAHLVQRSRQTIYNVYRF
                     LKSGGSAISYYEQYKKNKRNCGRRPIVLPDGQQEYIQKKVAQGWTPDVLIGRAEFPIA
                     CSVRTLYRMFKDKTFDPHDLPMKGKRKPNGHKEKRGKQAFRRSIQDRTTDYIQFNHEF
                     GHLEGDTIVGGKHKSAVITLVERLSKVIITLKPAGRQAIDIENRLNQWFQSVPKHLFK
                     SITFDCGKEFSNWKKISNTNDISIYFADPGTPSQRGLNEHSNGLLRKDGLYKAMDFNK
                     VSETFIQSVASKRNHIPRKSLHYRTPLEVFLSYVGKDDLSSLI"
     gene            complement(1527..2132)
                     /locus_tag="ACG8KR_002857"
     CDS             complement(1527..2132)
                     /locus_tag="ACG8KR_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0202155.1"
                     /translation="MINSAYFHIKYLKKYEYEEVVLDYGLNRQSFLIYAINNGNLPSN
                     KKNIIIETYAYLEGGSKKLIKSCNIEYQLSVGEIKCVFEEDLELDLKKIFASIHDYIG
                     LIYKIIEDDKQTELNMCYFDREQDFFYYGGMGGGSIVPKINLVELDVNNYRELYVFNS
                     LDFEIPMKQMKNIDIHILPSESCQIEYSIEYFYKVSFVKLS"
     gene            complement(2372..>2475)
                     /locus_tag="ACG8KR_002858"
     CDS             complement(2372..>2475)
                     /locus_tag="ACG8KR_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HFQ0202156.1"
                     /translation="WTCPICHTHHDRDINASINILTEGLRLHSMGLA"
BASE COUNT          825 a          406 c          384 g          860 t
ORIGIN      
        1 aacaataaat gaagactttt ttattgttaa ttgtttattt ttctattatt agatatgaaa
       61 atcgcattta gataaaatag aaaaatatga atatatttta aatattaaga tgagagtcat
      121 taacttatga tagtagtcaa tttgcttttc actaattaca aaatatgtga tataggaagt
      181 ttagttattg ttctcataat gccatcctgt ttttagatta tctagtaatt tttttgttat
      241 ttaaaattag gagcagtgcc aattgtttta attttaaagg ctttttttat atcttctaaa
      301 ttagaagata tttgtatatc aatatggaat atattacctt ttttgtattt ttttatgtta
      361 tctttagcta aaacatcaaa tacatgtcct tgaaattgta tcgaggcagc tggatttagt
      421 ttaaattgag gaaactttac cacagctgat tcaaaacact tgtcacattt ttttattttt
      481 ttaccttcgc cgattgtaaa attaagctag acaactaaaa aagtcatttg tgctacactc
      541 aaaatagttc cgtcgaaaga attataagga gtgagtacaa atgacctata cccatcttac
      601 accaactgaa ctcgtaatga tagaagctta tttcaatcaa tcacactctg tttcaaaagt
      661 agcccatctg gtacaacgtt ctcgacaaac catttataat gtctatcgtt ttcttaaatc
      721 tggtggaagc gcaataagct actatgaaca atataagaaa aacaaacgta actgcggaag
      781 acgtccgatt gtgttaccag atgggcaaca agaatacatt caaaaaaagg tggctcaagg
      841 gtggactcca gatgtcctta ttggtcgagc agagtttcct attgcttgtt cggtacgtac
      901 actatatcgg atgtttaaag ataaaacctt tgaccctcac gatttaccaa tgaaagggaa
      961 aagaaaacca aacggacata aagaaaaaag aggaaaacaa gcttttcgcc gttcgattca
     1021 ggatagaaca actgattata tacagttcaa tcatgagttt ggtcacctag aaggtgacac
     1081 cattgtcggt ggaaaacata aaagtgcggt gatcacgtta gttgagcgat tatctaaagt
     1141 gatcatcacc ttgaaacccg ctggtaggca agctatagat attgagaata gactgaatca
     1201 atggtttcaa tctgtaccga aacacttgtt taaatccatc acatttgatt gtggaaaaga
     1261 gttctcgaat tggaaaaaga ttagtaatac caatgatatt tcgatttatt tcgctgatcc
     1321 aggaacacct tctcaacgag gactaaatga acattcgaat ggcttattac gtaaagatgg
     1381 cttatataaa gctatggatt ttaataaggt atccgaaacc tttatccaat cggtggcatc
     1441 aaaacgaaat catattccta gaaaatcatt acactatcgc acaccattgg aagtattttt
     1501 gagttacgta ggtaaagatg atttgtctag cttaatttga caaacgaaac tttgtaaaaa
     1561 tattcgattg aatattctat ttggcacgac tcacttggca gtatatgtat gtcaatattt
     1621 ttcatctgtt tcataggtat ttcaaaatct aaactattaa aaacatataa ttccctgtaa
     1681 ttatttacat ctaactctac taaatttatc tttggaacaa tagatcctcc acccattcca
     1741 ccataataaa aaaaatcttg ttctctatca aaatagcaca tattaagctc agtttgttta
     1801 tcatcctcaa taattttata aatcaaacct atataatcat gaatgctggc aaaaattttt
     1861 tttaagtcaa gttctaaatc ttcttcgaaa acacatttaa tttctcctac tgataattga
     1921 tactctatat tacatgactt aattagtttt ttagatcccc cctccagata ggcatatgtt
     1981 tcaataatta tgttcttttt attgcttggc aaatttccgt tatttattgc ataaattaaa
     2041 aatgactgtc tatttagccc ataatctaat acaacttctt catactcata tttctttaaa
     2101 tatttaatat gaaaatatgc tgaattaatc aaaactcgag tgttactgtt gttatttatt
     2161 gttgttctat tgtgtataaa attgtatagg ttgtcatatt ttcttggttt agtgaactac
     2221 tcaccactta gctaaaccta acggtttctt acgtttgaag tgggagcttc ttgggaagag
     2281 agcatacttg atagcgtatt actttactaa cagaggtgcc tcttatttac caagctatcc
     2341 ccgtagttcc tacggttctt gtttatttga tctaagccaa tcccatagag tgtagcctta
     2401 gaccttcggt caggatattg atactagcgt tgatatctcg gtcatgatga gtatgacaaa
     2461 tagggcaagt ccatt
//
LOCUS       DAZKLD010000220         2407 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.225, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000220 DAZKLD010000000
VERSION     DAZKLD010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2407
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.225"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(101..708)
                     /locus_tag="ACG8KR_002859"
                     /pseudo
     CDS             complement(101..708)
                     /locus_tag="ACG8KR_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313657.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0019172 - glyoxalase III activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase family
                     protein"
     gene            1231..1905
                     /locus_tag="ACG8KR_002860"
                     /pseudo
     CDS             1231..1905
                     /locus_tag="ACG8KR_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297358.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
     gene            1898..2059
                     /locus_tag="ACG8KR_002861"
     CDS             1898..2059
                     /locus_tag="ACG8KR_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0202157.1"
                     /translation="MTEKEQVQQIVKKYNKSIADLSENASAKEFKTVMNYVADEANRK
                     QRKLVGLDK"
BASE COUNT          861 a          366 c          401 g          779 t
ORIGIN      
        1 tatgaatata aatagagtgt atcttctttg ataaatgtca gaaaaagtgt gacaaataac
       61 catcagtaaa attacttgtc atactattga taaggagtat ttaattgaag agaatgatta
      121 ggagaaagga tctccgtact ttttatagta atcatagaat ccaagtttgt atagacgaat
      181 ctttttttct cttatttctt ctgtttcaaa atggactgct tgaagaatca agtctgtgaa
      241 ttctatagga gctgttccat tcgcagtaat caaattacga tctttcacag cttgttgttc
      301 tataaaatct tctttattct gataattttc cgattctaac caaggaaatt gcgcatttcc
      361 ggtgtgtttg taaccttgca ataatccgct ctgagctaaa taatcaactg ccccacaaat
      421 agcagctact ggtacgtctg tttttaacaa tttatcgatt atttgatata aatgatgatt
      481 ttttatgtcc catgaattgc caccgatcat aataaaaagg tcaacttttt caggtatttg
      541 ttcgattaag taatcgattt ttgtttttaa tcctccaatg gaagtgacct ctgattggat
      601 agaagcagtt ttgttttgcc atccctcttt ttggtttagt gtgctagtaa tataagcacc
      661 ttcccaatcg gcatattcct ctaatagaaa cgtaatcgca gttttcattt tcaagctctc
      721 ctttactatc tatgacaagg acaacagttt taacatacag acattcatta aatttggata
      781 gcgacagcga aaacaacaat tcttaagttt tagacagagt atacagacag tcaacttaat
      841 taaatacgaa ctaaataaaa atgacaggta atcatttttg tttaccagca ttataacatt
      901 ttgcatacat ggatgacaaa agaaccaatt cccacttgct tgagtacaat cttaaaagaa
      961 actggaaggc aaaaagtaat tatagaaacg aaaaataaat catatcgggc actcagacat
     1021 gaaaacaacc ctagaaatat ataactagtc caatgaaaaa aaagcgaatg ccgtagaaaa
     1081 caaggattct gcgtacattc atatctcatt ttgcgagcat atctgctatt actgtgacta
     1141 aatgttaata tggttaaaat gaccgttata ttttttatat aaatatcgta taattagtat
     1201 gaatatagaa gaaggaaaga aggtgcaatc ttggaaccta aagacaagta tttagattac
     1261 gctagggtca ataaagataa atttattgaa acaatcatca agggaaaaat ccctgatgat
     1321 gaaaaagatg cggtattcat ggctggaagc ccaggagcag gtaaaacaga ggttgcacta
     1381 ggtttagctt aaaattatga caaccatgtt gtgattgatg cggactattt tagagcacaa
     1441 tttccagaat acaatggaaa gaactcaagt gtttttcaga aagcttcttc ttggttagtt
     1501 gaacaatcat taaaatatgt actggaacat ggctattctt tcatactaga ggatactttt
     1561 gctatcctta gtgcagagaa aaatattatt cgagccctta aaaataactt tcgcgttacg
     1621 attttctatg tttaccaaga tcccaaagtt gcctgggatt tcactcgaaa acgtgaacta
     1681 gctgaaggac gtcatgtgcc aaaagaaatg tttatcaatg cgttttttaa gtccagagag
     1741 aatattgaaa aagtcaaaaa tagacatcct gaagtagtcc ttcacataat gattaaggat
     1801 tatcaaaacg acatttctga agtgcattat gcagcagata atattcaatt agtactacca
     1861 atacaatata cttcaaaaga gttggaggag gaattacatg actgagaaag aacaagtaca
     1921 acaaatcgtg aagaagtata ataaaagtat cgctgattta tcagaaaacg cctcagcgaa
     1981 agaatttaaa acagtaatga attacgtagc agatgaagca aatcgtaagc agcgaaaact
     2041 agttgggttg gataaataaa ttttttaaac catatccttg taaagttgag ttttttattt
     2101 taatgaatta agactattaa atagataatt attgtaactt aaaaaacaca gctaaaaatt
     2161 agttgtgttt tttattaggt ttacttttct ggtataaaag gaactagcta ctttttcaaa
     2221 atttctaaat cattcaatat gtagcgctta ctaaattatt cgccataaaa ttttataatt
     2281 ggatcactca ttgttttacc taagtcatat ttaagtaata accggtgttt ttacataaga
     2341 agacttcggg tattttttag tttgattagc tctgatatac ctataaagaa tatgctaagg
     2401 taagaat
//
LOCUS       DAZKLD010000221         2403 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.226, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000221 DAZKLD010000000
VERSION     DAZKLD010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2403)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2403
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.226"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(844..1362)
                     /locus_tag="ACG8KR_002862"
     CDS             complement(844..1362)
                     /locus_tag="ACG8KR_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HFQ0202158.1"
                     /translation="MRTYKNKEELKKEINRTYTKYILEFETIPEELKDKRVEEVDRTP
                     AENLAYQVGWTNLILKWENDERNGLSVKTPSDQFKWNQLGELYKWFTNTYAHLPLKEL
                     EEILNRNIDDINMMIDSMSDEDLFTAHKRKWADEATKTAVWEVYKFIHVNTVAPFGTF
                     RTKIRKWKRLAL"
     gene            1729..1977
                     /locus_tag="ACG8KR_002863"
     CDS             1729..1977
                     /locus_tag="ACG8KR_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0202159.1"
                     /translation="MVKLKMLYIGLQHRSFFDCALGKKYYEVKIDDDTFLTAHQMKQL
                     QKRNDQLEENLILKKRLPYSRLARRTIKGQDLPEDHPF"
BASE COUNT          777 a          442 c          352 g          832 t
ORIGIN      
        1 tgatggtatt tagataacac gaaagaataa aaataacagc tgatagtaaa gtttgttgac
       61 gattgctact caatcgaaag tgagttagtg cgtatgaact tgcttctgaa aacttatgaa
      121 atgaaagacc ttttacatct tgcagaaatc gttactggga ttaaactaag caataaaaac
      181 taatgttccc ctcattcttt gaacttctac tatttgattt atttattctg ataaatgacg
      241 atttcacctt caggatcttc aaatcccgta tagaatttat ttaagatctc ttcagtatca
      301 gtaataggat aggcgatagg ccacataccc tttgatggtt agaataaact gctcaaaatc
      361 tgtatcgtat gttggcttta gcatattaac aaaggctatc tccaaattat aaaccataag
      421 cgttgaatcg ttcttaacaa tttcaattct tcctaaccca aagtcagaag atataattac
      481 cggacttgcc ttacccttca tgtgggttgt tgagattcca tatttaaagg gaattgtcgg
      541 tactatcggc ggtctgaaaa acatcccaaa aataaaaaaa tctttcccct gctagtctcc
      601 taagtccctt tgtagcggat atggcaccat aatctcattt tatcgaagtg agtcatggtt
      661 tatctgttgg ccaaccaaaa ttgatacaat ctaacgatgc accctctacg gcattctagg
      721 ggagtgcact tgcattcacc caataaaaac cggcagttag caaagaactt atgtagtcgt
      781 atgttcttac tgttaacata caagaggtct cctttccaca attgcttagc agtaaacggg
      841 aatttacaaa gctaatctct tccattttct tatctttgtt ctaaaagtcc caaatggagc
      901 aactgtattt acatgaataa atttatatac ttcccataca gccgttttgg ttgcctcgtc
      961 tgcccatttt cttttgtgtg cagtgaatag atcttcatca ctcattgaat caatcatcat
     1021 gttaatatca tcaatattac gattgagaat ttcctctaat tctttcaatg gtagatgtgc
     1081 ataggtattt gtaaaccact tatataactc tcctaactga ttccatttaa attgatcaga
     1141 aggcgtttta acagataatc catttctttc atcattttcc cattttagaa ttaaattcgt
     1201 ccaaccaact tgatatgcta aattttcagc aggtgttcta tccacttcct caactctttt
     1261 atccttcaat tcttcaggaa tagtttcaaa ttctaaaata tatttagtat aagtcctatt
     1321 tatttctttt tttaattctt ctttattttt atatgttctc attatattac ctccataaat
     1381 tcctaattgt ctcctttaat tcatctataa tttcatccac gatattttaa tccgttctac
     1441 aatgcatatc tgatatgcat tgactgtcta actcatatgt gaatgattta taaaatatcc
     1501 caacgtctca tctaaagaaa tgttgatcgt cagcaaggag ctaaaaaata cgaatatatt
     1561 tcttttgtag taaaatcgga ctggtttgca tatcattttc ttcgtaaata tcaaaatcat
     1621 gttaactact atcaattact aataaaaact accactttaa gcgttaagaa ctgttaagtt
     1681 tagttaagtg aggatagttc acaaaatcca ttgttaaact tcacgaaagt ggtaaaactc
     1741 aaaatgcttt atataggatt acagcatcgc tctttcttcg attgcgcgtt gggtaaaaaa
     1801 tattatgaag taaaaattga cgatgacacg ttccttacgg ctcatcagat gaaacagcta
     1861 caaaaacgaa atgatcaact cgaagaaaat ttaattctaa aaaagcgatt gccatattca
     1921 cgcctcgctc gacgaactat taaaggtcaa gatttacctg aagatcatcc tttttaggaa
     1981 attgaagctt tttatgttgc acaatgcatt tataatacta caatgatgtt ttcaccagat
     2041 attcttctag ggtgcggtgt aatgaaacag atacacttgc aaaagaaaat tcaccgcgaa
     2101 tttactaaaa ttatgaataa ttatgttaat cttccgaaat ttttttccaa aattaaggga
     2161 tcaagctggt gttatagatt gtttaaggtt ggcaaaaaaa taacttcttt ttaaacaatt
     2221 atagaaaacc gatactctgt taagttggtt gacacggtgt cgatttttat gactttctta
     2281 ttctgaaact tcactaacgg acagtaccta aaaagtctat agtatggacg tttacgagta
     2341 ctattttatc aaaaatgctg ctcatttcaa cttatatgtt ttagtgaact acccccactt
     2401 agc
//
LOCUS       DAZKLD010000222         2400 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.227, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000222 DAZKLD010000000
VERSION     DAZKLD010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2400
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.227"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1097)
                     /locus_tag="ACG8KR_002864"
     CDS             complement(<1..1097)
                     /locus_tag="ACG8KR_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HFQ0202160.1"
                     /translation="MARKKRNPDAEKLAESILNTYQPESVEDMQDVLKDVFGPLFEKM
                     LQGELNNHLGYDAHSKEPKKHDNRRNGYGNKTLKTSFGKVNIDVPRDREASFEPELIP
                     NRKRDVSDIEGKVLSMYARGMSQCDIAATVEDIYGFDISHEMISDITDAVLPELEEWQ
                     ARPLAKCYAFLFVDCMYVTLRENYEAKEYAVYTILGYDLKGNKEILGLWLNQTESKNR
                     WMQVFDELKVRGVEDVFFISMDGVSGLKEGAKAIFPSVIVQRCIVHLVRNALRYLPSK
                     DYKEVCRDMKKFYSASSLNAAHAAFDSFQNRWSHYSGAVDVCKRNFSHVEQLFDYGSA
                     IRKIMYTTNAVESIHSSFRKVTKKGVFSNENA"
     gene            complement(1227..>2400)
                     /locus_tag="ACG8KR_002865"
     CDS             complement(1227..>2400)
                     /locus_tag="ACG8KR_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0202161.1"
                     /translation="RGGGNVKGEDIKKISQTDSIDSYVKRINSVADLVDYDIIETQET
                     LANQSPERAKNFKRTVMLTGVNDSTKETKFVSEAYKLVEGKHLENEDKNKILMHKDLA
                     EKNNLKVGDKIKIKSNLFDADNEKGADETVEVEIKGLFDGHNSGGVSAAQELYENTLI
                     TDIHTAAKVYGNTEDTAVYQDATFFVKGDKNLDSVIKDLGKLDINWREYNLIKSSSNY
                     PALQQSISGIYSISNKLFFGSLIFAGVVVSLLLFLWMNARKKEIAVLLSLGISKLEIF
                     GQFLIEMVFISIPAFVGSYFLAQYTADKLGNNILNKVTGDIAKQIAKQSASSQLGGGA
                     EAEGFNKTLSSLDINVLPKFMIYVVIFMSLVLLVSLIISSFNILRRNPKELLIDNN"
BASE COUNT          730 a          468 c          353 g          849 t
ORIGIN      
        1 gcattctcat tagagaacac tccctttttt gtgacttttc tgaagctaga gtgaatactt
       61 tcaactgcat tagtagtgta catgattttt ctaatggcgc tgccataatc aaagagttgt
      121 tcaacatgag aaaagtttcg tttgcataca tctactgcac cagaataatg agaccaccga
      181 ttttgaaaac tgtcgaaggc agcgtgtgca gcgtttaaag aagaagcact gtagaatttt
      241 ttcatgtctc gacagacttc cttataatcc ttacttggga ggtatcgtaa cgcatttcga
      301 acaagatgaa caatacagcg ctgcacaatg acagaaggaa aaattgcttt tgcgccttct
      361 ttgagaccgg aaacaccgtc catcgaaatg aaaaaaacat cttctacacc acgtactttc
      421 agttcatcaa atacttgcat ccagcgattt ttagattctg tttgattgag ccataggcct
      481 aaaatctcct tgtttccttt aagatcatag ccaagaatgg tgtacacagc atattcttta
      541 gcttcatagt tttcgcgtaa agtgacgtac atacaatcaa cgaatagaaa tgcataacac
      601 ttggctaatg gccgagcttg ccattcttcc aattcaggaa ggacggcatc cgtgatatca
      661 gaaatcattt cgtgggaaat atcaaagccg taaatatctt caacggttgc ggcgatatcg
      721 cattgactca ttcctcgtgc atacatggaa agaaccttac cttcgatgtc ggagacatct
      781 cgttttctat taggaatcaa ctcaggttca aaagaagcct cccggtctct gggaacatca
      841 atgtttactt taccaaagct cgttttaagc gtcttattgc cataaccgtt tcgtcgattg
      901 tcatgtttct ttggctcttt agaatgggcg tcatacccta aatggttatt caattctcct
      961 tgaagcattt tttcaaataa gggaccaaac acatctttta aaacatcttg catatcctct
     1021 acagattcgg gttgataagt attcaaaatg gattcagcta acttctcagc atcaggattt
     1081 cttttctttc tagccatcgt gtaatcacct cttgatctta ttttagaaaa ataaaaaccg
     1141 tgaagcaacc acccgcctag gattgatggt tacttacacg gtttatatta cactctcatt
     1201 tttggtgcta aaaagcaccg aaaattttaa ttattgtcaa ttaataattc ttttggattc
     1261 cttcttaata tattgaatga agaaataatc aatgatacaa gaagtactaa actcataaat
     1321 ataactacat aaatcatgaa tttaggtaat acattgatat ctaaacttga taatgtcttg
     1381 ttaaatcctt cagcttctgc cccaccacct aattggctag atgcagattg tttagctatt
     1441 tgtttagcta tatcaccagt aaccttattc aatatattat tcccaagctt gtcagctgta
     1501 tattgagcta agaaatagga accaacgaat gcggggattg atataaatac catctcaatt
     1561 aggaattgac caaaaatttc taattttgat atacctaatg atagtaatac tgctatttct
     1621 ttctttctag cattcatcca taagaataat aataatgaaa ctacaactcc tgcaaatatt
     1681 aatgagccaa aaaataattt atttgaaatt gagtaaatac ctgatataga ttgttgtaat
     1741 gcagggtaat ttgatgaact tttaatcaaa ttatattctc tccaatttat atctaatttc
     1801 ccaagatctt ttattacact atcaagattc ttatcacctt ttacaaagaa tgttgcatct
     1861 tggtatacag ctgtatcttc ggtattacca taaactttag cagctgtatg aatatcagtg
     1921 attaatgtat tttcgtatag ttcttgtgct gcacttactc caccgctatt atgcccatcg
     1981 aatagacctt taatttctac ttctactgtt tcatctgcac ctttctcatt atcagcatca
     2041 aataaattag attttatttt tatcttatct ccaactttaa ggttattttt ttcagctaaa
     2101 tctttatgca ttaagatttt atttttatct tcattttcta aatgctttcc ttctactaat
     2161 ttatatgctt ctgatacaaa ttttgtttct tttgttgagt cgttaacccc agttaacata
     2221 actgttcttt taaaattctt tgctctttca ggtgattgat tcgcaagagt ctcttgagtt
     2281 tcaataatat cataatcaac taaatctgca acactgttta ttctttttac ataggagtct
     2341 atactatctg tttgagatat ctttttaata tcttcacctt ttacatttcc cccacctcta
//
LOCUS       DAZKLD010000223         2388 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.228, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000223 DAZKLD010000000
VERSION     DAZKLD010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2388)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2388)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2388
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.228"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            197..2059
                     /locus_tag="ACG8KR_002866"
     CDS             197..2059
                     /locus_tag="ACG8KR_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFQ0202162.1"
                     /translation="MQQLTKRERQLLIYLVEMSEPVTIKKLAEKENVSVRTIKYDLDD
                     IREWLNERKQVLYSKRSQGIWMNISSSDRIKLKSELMDVNRFEIFADQSLRIKRLLIM
                     LLVTKEAITGMKMANALDVSKDTIMNDLDVLENNFLKEGLTLNRQARKGFWIIGEERL
                     VRLTIEEILQKEFTDYDIYKLMSLLLNGGETEYFEMYSATATPIQEVFNQVIIRMRHL
                     LEFENLEKLNYAELPNILIRVTIATVRLRKEATIGRYQLVAEQELDKNDLSYRVMQQV
                     FDYYQLPLFEDEYHYIYSDTFDTIHQQDVMKLVENVIYKVSKAINHSFYQDPQLLTNL
                     YAHLSMRLSRKKKFVNEYNPFKEDIKQKYPELFDAIKQAVRQEISEQNILINDSFVAY
                     IALHFLVSYEREEDLRSVRAVYICSTGLGVTSLIKQKINEELNNITIVAFASVLNAQE
                     MIATKDPDLVISIFPINGIDRPFIKVHPLPTEQDLKKIQQIIKKILASSNQQERHNLN
                     LRETSINKDTLEDFSKELILKAYTIYERLLVILEAELIQEYREAFLLHVFLMVHRITF
                     DQQYMAEGRQVDQIILDKGTLVGKIEAVFAENNLGINQSEISALFSYIKEGASS"
     gene            2059..>2388
                     /locus_tag="ACG8KR_002867"
     CDS             2059..>2388
                     /locus_tag="ACG8KR_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFQ0202163.1"
                     /translation="MKMSDEALKIIEESQNREKLKQVIDLIEERLVEHAIVPTELQWT
                     ILINHLNEMLKRSQRKETIPVVDRDLFKEVSKEAITISDEIVRSIGNLTDDEIYVLSI
                     HFETAKNN"
BASE COUNT          859 a          354 c          465 g          710 t
ORIGIN      
        1 aaaagcgttt gattatttat attacgactt agtgcaataa aaaacgcttt ttaacagtac
       61 atgtagtcgt ctttataata gataaactct gtaatattag ttaaaaagct ataagttgca
      121 cttagttaag tgcaaacatt tcatattgtc gttagtttag aaaacgctta tattagcaat
      181 gtaaggagag atgataatgc agcagctaac aaaaagagaa aggcagcttt tgatttactt
      241 ggtggaaatg tctgaaccgg taacaatcaa aaagttggct gaaaaagaaa atgttagtgt
      301 tcggacgata aaatacgatc tagatgatat aagagagtgg ctgaatgagc ggaagcaagt
      361 cctatatagc aaacgtagtc agggaatttg gatgaatatt agtagctctg atcgaatcaa
      421 attaaaaagc gaattgatgg atgttaatcg ttttgaaata tttgcagatc aaagtctccg
      481 aatcaaacgt ttactaatta tgcttttagt aacaaaagaa gctattactg gaatgaaaat
      541 ggccaacgca ttagatgtca gcaaagatac gatcatgaat gatttagatg tgctggaaaa
      601 taattttctt aaggaaggat tgaccttgaa tcgtcaggcg cgaaagggtt tttggatcat
      661 aggagaagaa cgtctagttc gattaacgat tgaagagatt cttcaaaaag agttcacgga
      721 ttatgacatt tataaattaa tgtcattgtt attgaatggc ggtgagactg aatactttga
      781 aatgtattct gctactgcta cacctatcca agaggtattt aatcaagtaa tcattcgaat
      841 gcgacactta ctggaatttg aaaatctaga gaaactgaat tacgcggaat taccaaatat
      901 tctgattcga gtgacaattg ctaccgttcg cttacgcaaa gaggctacga ttggtcgtta
      961 tcaattggtt gctgaacaag aattagacaa aaacgatctt tcttatcgcg tcatgcagca
     1021 ggtctttgat tattatcagt tgccactgtt cgaggatgaa tatcattata tctatagtga
     1081 tacattcgac acgattcacc agcaagacgt catgaaacta gttgagaatg tgatttataa
     1141 agtaagtaaa gcaatcaacc attcttttta tcaagatcca caattgttga cgaatctgta
     1201 tgctcattta tccatgcgat tatcgagaaa gaaaaaattt gtcaatgaat ataatccttt
     1261 taaagaagat atcaagcaga aatacccgga attatttgat gcgatcaagc aagcagttcg
     1321 acaagaaata agtgaacaaa atattctgat caatgattct ttcgtggcat atattgcctt
     1381 acactttttg gtttcctacg aaagagaaga agatctacga agcgttagag ccgtctacat
     1441 ttgttcaaca gggttgggtg tgactagtct gatcaagcaa aagatcaatg aagaattaaa
     1501 taatatcact atcgtggcat ttgcttcagt attgaatgcg caagaaatga ttgctaccaa
     1561 agatcctgat ttggtgatta gtatttttcc aattaatggc atcgatcgtc ctttcatcaa
     1621 agtccatcca ttacccactg aacaggactt gaaaaaaatt caacaaataa taaaaaaaat
     1681 acttgcttct tccaatcagc aggaaaggca taacttgaat cttagagaaa cgtccatcaa
     1741 taaagatact ttagaagact ttagcaagga attgattttg aaagcttata cgatttatga
     1801 acgtttactt gtcattttgg aagcagaatt gatccaagaa tatcgagaag cttttttgct
     1861 tcatgtattc ttaatggttc atcgtatcac atttgatcaa caatatatgg cagaaggtcg
     1921 tcaagtagac cagattattt tagacaaagg aacactggta ggaaaaatcg aagcagtttt
     1981 tgcagaaaat aatttaggaa tcaatcagtc agaaatcagt gcattattct catatataaa
     2041 agaaggagcg tcaagttagt gaaaatgtca gacgaggctt taaaaattat cgaggaaagt
     2101 caaaatcggg aaaagttaaa acaagtgatt gatctaattg aagagcgatt agtagaacat
     2161 gctattgtac caaccgagtt gcagtggacc attctaatta atcacttgaa tgagatgctg
     2221 aaacgttctc aaagaaagga aacgattccc gtagtcgatc gggatttgtt caaagaagta
     2281 tccaaagaag ccataactat ttctgatgaa atagttagaa gtatcggtaa tttaaccgat
     2341 gatgaaatat atgttttgtc gattcatttt gaaacagcaa aaaataat
//
LOCUS       DAZKLD010000224         2322 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.229, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000224 DAZKLD010000000
VERSION     DAZKLD010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2322
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.229"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(274..1461)
                     /locus_tag="ACG8KR_002868"
     CDS             complement(274..1461)
                     /locus_tag="ACG8KR_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit R"
                     /protein_id="HFQ0202164.1"
                     /translation="MQNTAHKNPYKTVDEATQLDLVIVVNQLLTGYDSKWVNTLYLDK
                     SLEYQNIIQAFSRTNRLNGPEKPFGTIRYYRKAHTMEQNIEEAIELYSGKQAKGLFVE
                     KLYNNLEKLNDIFEEIKSVFNISGIENFSHLPEVPGEIKQFVNLYNQLIHCYESAMIQ
                     GFTWAKSTYEIQDYEPITVVITEYEFNILAQRYSEIEREPRDGNDSIAYDIDYQLPEG
                     SRSRINYEYLNTNFKKYMNIKEERDSNLIQSLLNDLHRTFAQLPVELQPYAEMILNDV
                     NNGELVIKEGWEFTDYLNEYQLSEEDDFIQDLVDSTNINEALLREMLDLRLTEVNINE
                     YSRFDKLKSSVNVGHFSGYIEKNLGKMVIPIKVNMLIDRLLRAFLLEDVFDVTEYIKN
                     YFN"
     gene            1497..2270
                     /locus_tag="ACG8KR_002869"
                     /pseudo
     CDS             1497..2270
                     /locus_tag="ACG8KR_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
BASE COUNT          719 a          441 c          369 g          793 t
ORIGIN      
        1 attttaatac aatttatgat acaaaaaatg acctcctttc ttaaataaaa gaggtcattt
       61 tgttttgtct cataatcgat tgtttttgat acagtagtta tattcagaaa aaataaagac
      121 tcttctgagt ttataatcct ttggattata tacggataca gatgtagaca taaaacaagc
      181 tcaattttca aaaatggcat tactcacctt tttggtgaat tattaaacta aaaaagatga
      241 atttttttat gtactataag atgtaggtgt actctagtta aagtaattct ttatgtactc
      301 tgtaacatcg aatacatctt ctaataaaaa tgcacgcaat aatctgtcaa ttagcatatt
      361 taccttgatt ggtattacca ttttacctaa atttttctcg atatatccac tgaaatgacc
      421 tacgttaaca cttgatttta atttatcaaa tcgactatat tcattaatgt ttacctcggt
      481 taagcgtaaa tccaacatct cacgtaataa tgcctcatta atattggttg aatccactaa
      541 atcttggata aaatcgtcct cttcactaag ctgatattca ttgaggtaat cagtaaattc
      601 ccatccttct ttgataacta attccccatt atttacatca tttagaatca tctcagcata
      661 tggctgtaac tccactggta attgcgcaaa tgtgcgatgt aagtcgttta acaatgattg
      721 aattaaattc gaatcacgtt cttcttttat attcatgtat tttttgaagt ttgtgtttaa
      781 atactcataa ttaatacgac tacgactacc ttcaggtaat tggtaatcaa tgtcatacgc
      841 tatactatca tttccgtctc taggttctct ctctatctcg ctgtaacgtt gtgccaagat
      901 attaaattca tattctgtga ttactactgt tataggttca taatcctgga tttcataagt
      961 cgattttgcc catgtgaatc cctgtatcat cgctgattca taacaatgga ttagttgatt
     1021 ataaagattt acaaattgct taatttcccc tggaacctcc ggtaaatgac tgaagttttc
     1081 tatgccagaa atattaaata cggacttaat ttcttcgaat atatcgttca acttttctaa
     1141 gttattatat aatttttcaa caaataaccc ttttgcttgt ttgcctgagt ataactcaat
     1201 agcctcttca atgttctgtt ccatcgtatg tgctttacga taataacgaa tggttccgaa
     1261 cggtttttca ggaccattaa gacgatttgt gcgtgaaaaa gcctgaatta tgttctgata
     1321 ttcaagtgac ttatctaagt atagggtgtt gacccatttt gaatcgtaac ccgttaagag
     1381 ctgattaaca actataacaa gatctaattg ggttgcttca tctactgttt tgtaaggatt
     1441 cttatgcgct gtgttttgca atagttttgt acacaagatt gcaaagaaaa gtacacaatg
     1501 ttgccacttc tggttccatg agttttattt gtgttgatgg gacaaccaat cagctattta
     1561 cgacccttga agatcgcaga ctttacaaac taactcaata cttcctgcgc tttcctagaa
     1621 aggcccgttt aaaggtcaag tacctagtga tggacatgaa tgccagctat tgtcagttgc
     1681 ttaaaacggt ctttccaaat gcggagatcg taactgaccg ctttcatatt gtccaacaca
     1741 tcaatcgcag cttcaatcaa ttacgtgtcc agatcatgaa tcgttttcga acctcacact
     1801 ctgaaaatca gaagaagtac cgtcggctca agcgatattg gaaactctta ctcaaagatt
     1861 ccacgacatg ggaggcactc aaacggcatt atcatcgact gtttaaacga ccaattagtc
     1921 aaacagaaat cgttaatgag ctgttaagct acaacgaaga attgcgaact gcgtatcatt
     1981 tttgtcaatt attacgctat tatttcgtta aacgagatac ggtaggcttt caagaaaccc
     2041 ttaaaacaat ttcatctacg ttacctcagt cgtttaaaaa gaaattcacg atcttccatc
     2101 gctaccaatc gggtatttca aatgccttta aggtgtccca ctcaaacggc gtcactgagg
     2161 ggctgtataa caagatcaag ctgattaaac gaattgcgtt tggttatcgc aacttttata
     2221 actttcgggc acggatctat ttacaacaag gtctgatctt tgaaaactaa aaaaatatgc
     2281 ggcgcacaga acgcgccgca tatcggacta agttttattg gc
//
LOCUS       DAZKLD010000225         2316 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.230, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000225 DAZKLD010000000
VERSION     DAZKLD010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2316)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2316)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2316
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.230"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            288..542
                     /locus_tag="ACG8KR_002870"
                     /pseudo
     CDS             288..542
                     /locus_tag="ACG8KR_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002405927.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
     gene            612..1124
                     /locus_tag="ACG8KR_002871"
     CDS             612..1124
                     /locus_tag="ACG8KR_002871"
                     /inference="COORDINATES: protein motif:HMM:NF013677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFQ0202165.1"
                     /translation="MTNKNNERYSTELKATAIKKMMPPENRGIRQLSQTLGIPEATLY
                     SWRKKARIQGNTTPGTKKNVEKWSSEDKFLIVMESFSMNQVELAEYCRKKGLFKEQIE
                     AWKKTCLSANEQEENRTRELATELKEEKKQARQLEKELRHKEKALAEAAALLLLRKKA
                     HAIWGDQEEE"
     gene            1121..2158
                     /locus_tag="ACG8KR_002872"
     CDS             1121..2158
                     /locus_tag="ACG8KR_002872"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFQ0202166.1"
                     /translation="MISSSDRKQAVELIQEANRNGARLTYACNELNISVRTYERWTRE
                     GTIAHDQRPLAKRPVPKNKLTDQEREKIIETVSKKEFMNLPPSQIVPKLADCSIYIAS
                     ESSFYRVLREKNMQHHRGRSQVSQKRIPPSHLATKPNEVWTWDITWLKGPIKGLFYRL
                     YLIIDLFSRKMVGWEIWESEEARHAEKLIKQTVLKERIQGQPLVLHSDNGSPMKAATF
                     QVLLEKLGIQSSYSRPRVSNDNPYSEAAFRTLKYRPEYPNNGFKTIDDARKWTKEFVD
                     WYNDSHLHSGIQFVSPAACHEGRHLAILEKRKQVYEDAKKRHPERWSKGVRNWTPHQS
                     VALNPLKETES"
BASE COUNT          842 a          419 c          465 g          590 t
ORIGIN      
        1 ataaattatc taaagagaat taagttttga ttatgtaata agttttggta ctaaaaaaga
       61 cttgatctaa ttagaccaag tcttttgtta gtgttatatt aataacaaaa taaaaataag
      121 aagtcgctca ctccccgacc aaagtttgtg agcaaaataa acagtcgatg tttgtttctt
      181 aattgtatat cttgatatta aacgatttaa gattattctt caagatatat attcgcaaat
      241 gtaattggaa aagcgacttc ttaaacaaaa ttaaggagtc gctttttatg gcaaaaataa
      301 cgaaaaatga gcaagaaaat ttggtggaaa attacacaaa aaaaccggcg tactctaata
      361 gccggttaga cgcacatttc gtgtgcactt ctaatccgaa attgagcttt gacgctatga
      421 cgatagtcgg aaaccttaat aaaaacagcg ctaaaaagct atccgatttt atgagtttag
      481 atccacaaat tagactttgg gatatacttc aaacaaaatt taaagctaaa gcactacaag
      541 accccctatg ttaggctagt tgtcgtatag gataataagt tataataaag actatacgga
      601 ggatgattac catgacaaat aaaaacaatg aacgttactc taccgaactg aaagcgacag
      661 ccatcaagaa aatgatgcca cccgaaaata gggggattcg ccaattaagt caaactttgg
      721 gcattccaga agcgactctt tattcttggc gtaaaaaagc tagaattcaa gggaatacta
      781 cacccggaac gaaaaaaaat gtcgaaaaat ggtcaagtga agataaattt ttaattgtga
      841 tggaatcatt ctcaatgaac caagtagaac ttgcggaata ttgccgaaag aagggcttat
      901 ttaaagaaca aattgaggct tggaagaaaa cgtgcttatc tgcgaatgaa caggaagaaa
      961 atcgaacgag agaacttgca actgaactaa aagaagagaa aaaacaagcg cgtcaattag
     1021 agaaagaatt acgtcataag gaaaaagcat tagcggaagc cgctgctctc ttattactaa
     1081 gaaaaaaagc acacgcgatt tggggggacc aagaggaaga atgatttcat cttcagatcg
     1141 caaacaagct gttgaactta ttcaagaagc caatcgcaat ggggcccgtc tcacctatgc
     1201 ctgtaatgag ctgaatatta gcgttcgaac ctacgaacga tggacgcgtg aaggtacgat
     1261 agcgcatgat caacgccctt tggcgaaacg tcctgtaccc aaaaataaac tgacggatca
     1321 agaacgcgaa aaaataatag aaaccgtttc aaaaaaagaa tttatgaatt tgccacctag
     1381 ccaaattgta ccaaaacttg cggattgctc gatttatatt gcatcagagt ctagcttcta
     1441 tcgtgtattg cgagaaaaaa atatgcaaca tcaccgtggc cgaagtcaag tttcacaaaa
     1501 acgcataccg ccaagtcacc ttgcgacgaa gccaaatgaa gtttggactt gggatattac
     1561 ttggctaaaa ggtccaataa aaggactctt ttaccggtta tatctcatca ttgatttatt
     1621 tagccgtaaa atggtaggct gggagatttg ggaatcagaa gaagctcgtc atgccgaaaa
     1681 acttattaaa cagacggtgc ttaaggaacg tatccaagga cagccgttgg ttttacactc
     1741 cgataatggc agccctatga aagccgcgac ttttcaagtc ttactcgaaa aattaggaat
     1801 tcaaagttct tattcaagac cacgagtgag taatgacaat ccttattcgg aagcggcttt
     1861 ccgaacgtta aaatatcgac cagaatatcc caataacggc tttaaaacaa tagatgatgc
     1921 tcgaaaatgg acgaaagagt ttgtcgattg gtataacgat tctcatttgc atagtgggat
     1981 tcaatttgtg tctcctgcag cgtgtcatga aggacgacat ctcgctattt tagagaaacg
     2041 aaagcaggtt tacgaggacg ctaaaaagcg ccatcctgaa cgctggtcaa aaggtgttcg
     2101 aaattggact ccacatcaat cggtggcgtt gaatccttta aaagaaacag aaagttaaca
     2161 ggagcataat ctttcaagat tatcaaataa ccctcgtcac ccaatagggg aaaaagttat
     2221 tcttccccta ttggataacg aggccaaccg accataggga ggttctgctt tttgataggt
     2281 ggaaaataaa aatacgacaa ctatattgac aaacac
//
LOCUS       DAZKLD010000226         2315 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.231, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000226 DAZKLD010000000
VERSION     DAZKLD010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2315)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2315)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2315
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.231"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..300)
                     /locus_tag="ACG8KR_002873"
     CDS             complement(<1..300)
                     /locus_tag="ACG8KR_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6056 family protein"
                     /protein_id="HFQ0202167.1"
                     /translation="MNKHNRKITNNKELILLLIGIWGIFLLFNMYMPTMRADDVVYAS
                     RLDELGYLGASIEHYKTWSSRIIIELFLMFFSKHFMLWKLLNSTIMLGIVVLLCKY"
     gene            393..742
                     /locus_tag="ACG8KR_002874"
                     /pseudo
     CDS             393..742
                     /locus_tag="ACG8KR_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741963.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            complement(1014..1304)
                     /locus_tag="ACG8KR_002875"
     CDS             complement(1014..1304)
                     /locus_tag="ACG8KR_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0202168.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            complement(1512..2203)
                     /locus_tag="ACG8KR_002876"
                     /pseudo
     CDS             complement(1512..2203)
                     /locus_tag="ACG8KR_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
BASE COUNT          668 a          435 c          334 g          878 t
ORIGIN      
        1 atacttgcaa agtaatacta caattcctaa cataatcgta gaatttaata atttccatag
       61 cataaagtgt ttagaaaaga acattaagaa tagttctata ataattcttg aactccatgt
      121 cttataatgt tctatcgaag cacccaaata gccaagttca tctaatctac ttgcataaac
      181 tacgtcatcc gctctcatag tcggcatata catattaaag agtaaaaaga taccccaaat
      241 ccctattagt aacaagatca gttctttgtt attcgttatt tttctattat gtttgttcat
      301 ttttatactc cttcttatat ttcctaatag agtataacat agctttaagc attacttaaa
      361 gataacttaa aagttatctt ttaaatactt aattgttaaa taccttattt gatgcaaaag
      421 aaatctacga tagtcaacaa aaaaattttc attatatcca gaaaaatttt tcttagttaa
      481 ggacctatgg attgcagtaa tgcagaattc gtcgaataaa ctgccaaaga catacaatca
      541 tattgctttt aaaattgacg aacaagatat agattcgttt gtctcaaaaa tccaaatgtt
      601 aggtttaact gtcgaaccag gacgttctcg tgtgaaaggc gaggcttctt caatttactt
      661 ttacgactat gataatcacc tcttcgagtt acataccggt actctccagg aacgtttaaa
      721 atcctacaat tccactacct gagactagta atgactagtc tctttttatg gcctctgatc
      781 cccccattga gcgaatacta atagggcttc gaattgatct gttcaatctt taagcaatat
      841 ccctcaaatg agtttggaaa gtttagtgat tcatcacaaa tacttaaata catgtagtta
      901 tatatagatt gaggttcttg tgagcgtatg ctcgtgtggg ttcaaatccc atcgtctgca
      961 tccatctttc caaaacaagc ggctaggaca tttttgtctc agccgcttgt tttctagttt
     1021 attctttcat cttgtcggac cttttttctt gggtatagac ttttctcata ttctaccaca
     1081 attttgggtt tccctaacac atgataggat ctcacaacaa attgctttct attattataa
     1141 taacctgtaa cgactaactt catcccttcg tctacttcag ctaggaagtt caaactatgg
     1201 gaagcaatca aacaattgac cccatttaaa ctgaaacgga tcaacggtct ggcactaact
     1261 tgcaaagtac ggatcttatc tactgttcca tattttttct ccataacctt cacctcaata
     1321 ccattatacg aacatacatt ccctttgtaa agaagaaact ctgttttatc tttttgactt
     1381 tttgtctatt acctttctta aaatcatatt ggcacttcct gcgaagatga aggacatgta
     1441 ctaaaaaaaa gaggcagcag tatagattac tgttgcttct ttactaattt aggtttactt
     1501 gttaagaaag attatttacg caaatgttct tcaacaattt gctgagattt atcagctgta
     1561 tcttccactt catcttttgc tttttctaac ttttgaatat attgtttcaa gcttgagttt
     1621 tcagcagttg cttcgtctaa tttcttttgt gtatctgtta actgatttct acatttgttt
     1681 tttcttgtgt cagtgttgcg atttgttgat tcaaaccatt tacagtattt tgtaattctg
     1741 aaagttttgc ttgtgcttct tgtagtcttg ctgtccaatc tgcattcaga tgattgattt
     1801 catcattttt ttggttgatc tcatctagtt tgacattgat ttcattttgt ttgctttgta
     1861 gttgtgcatc cttgctgact aattgacttt ctaagttttg tttttctgct gccgattgat
     1921 ttttttggtt ttctaattca tttacttttg tttggtattc ttggacatct gcctttgaat
     1981 cttgcaatgt ctgttctttt tgtgctagtc gagcggtaag atctgtaatc gcttgctctt
     2041 tatctgtcaa tgtatgatca agctgtgtca gattctcatt gattgtatca atatctgtct
     2101 ctccacccca caaaataatt tcatttgcgt accgcgtgcc tgtgtagcca attagacata
     2161 gaaatgcaac gacaatccca ccagtaataa atttcttttt catttgcata gctccttaat
     2221 ccaatacttt tacatgctaa aagtaagtga tctcaaacac aatgtcaagc gtttttaaat
     2281 tacatatgta attttaatat aaatactatc aaata
//
LOCUS       DAZKLD010000227         2314 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.232, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000227 DAZKLD010000000
VERSION     DAZKLD010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2314
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.232"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(616..801)
                     /locus_tag="ACG8KR_002877"
     CDS             complement(616..801)
                     /locus_tag="ACG8KR_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0202169.1"
                     /translation="MSKNSKGFLTILLAFIGYMLVGLLKSYSNELLNFSTFINDTLVP
                     SLFFIVFFAVGYFIIKI"
     gene            complement(1651..1992)
                     /locus_tag="ACG8KR_002878"
     CDS             complement(1651..1992)
                     /locus_tag="ACG8KR_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFQ0202170.1"
                     /translation="MSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRK
                     TMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQLKSLDFKERKLKKIENLPLQDM
                     AKIDQIIQYMF"
     gene            complement(1992..2222)
                     /locus_tag="ACG8KR_002879"
     CDS             complement(1992..2222)
                     /locus_tag="ACG8KR_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFQ0202171.1"
                     /translation="MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLV
                     PKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL"
BASE COUNT          772 a          406 c          314 g          822 t
ORIGIN      
        1 tttatttatt agatattcaa cagaaatccc gataacacta gccaacaata tatttatcag
       61 aagtgtaaaa taaggatgct ttttatagaa tagaactagt gttgttttca tagactctcc
      121 tttcttcata tttccaatta atcacaaatt cacggcatta ctagaaaaat taaaggggcg
      181 cagaagtgga aaaatactct tcgactttcc ctacaaacca ggccatcttg tgtttaataa
      241 ctataccaaa tctaaggtac ctctaaatct aaattccatt tgtggcggtg acctccaatt
      301 tttaactctt ctatctccca tccagtatac ataaccttga tacttcgacc acttatatat
      361 cgaacacaca aatacctatc cattgcagga gtactaccta acttagttgc tacttgcatc
      421 taaataacag ctgaaaagat aggacttact cttagtaaag tatctgaact attaattttt
      481 aaactatcat ttttatcctc aactacatgc acatctgttg caacttcact ttctgtctca
      541 tccagataaa tgattaagga atcgggaatt gcaatatctt cagctgacgc tggaatagtt
      601 gagctaaatt agtcatcata tctttattat aaaataacca acagcaaaaa atacaatgaa
      661 aaacaaactt ggaactaatg tatcattaat aaatgttgaa aaattcagca actcattact
      721 gtatgatttt agcagcccaa ccaacatata ccctataaaa gctaataaga tggttaggaa
      781 tcctttagaa tttttactca ttcatagaca cttccttaaa taacttttat tatagaaggt
      841 attgtaaata ataatattga caaaaagtga ctcaaaataa aaaaaacctt acattatact
      901 ggaatgaact gtattgatag tgtacctaaa agctctactg acataagatc gtgtaatcgg
      961 taaagtaatt ttattaatta tacgggatgt aatcaattag agaataaact aatcataaaa
     1021 ggtactttta cattcataat tgtcgctttg agattctact ttggttcact atctgaccca
     1081 attgcacatt ttcgatcatt acaagtattg gtgtcgttag aacaactaaa taaaaactag
     1141 cggtagattt ttcagaatct atgctaattt ctgtatggat gttccagtga tggcaaattt
     1201 ctcttttcat tgtttttctt gtcgcatgat atgccttttt gtgttattca gttaaactat
     1261 agaaattctt tcctagacag atcttgatcc caaattttac gtttttgagc atcgatatag
     1321 tttaaaatga cattactcat atctaaagag tttagatttt ttaggactaa aactgtcagt
     1381 tgttcttttt cttctttctg ttaaagtatt gtcgcgactt ttcgtatacg ttaagatatg
     1441 tcgtgaaaaa acaagttttg tgacacaaaa aaacgaatga cttgaggaaa tcacctcaat
     1501 cattcgttta aaatgctata tattctttag tttaagatac ggaaattaat gccatcgacc
     1561 acactagtag aatgaacgac atttacttgg ccttttttta atttcgttat gtttagcata
     1621 taaaaatatt atagagttta cttacaatgc ttaaaacata tactgtataa tctgatcaat
     1681 tttagccatg tcttgcaatg gtaagttttc tatttttttg agttttcgtt ccttaaaatc
     1741 aagagatttt aattgtgtaa taattacttg tccttttata tctagctctt caggtaatga
     1801 gtaacgtgtc ggtaaattct ttatagtgga agtaattgga catatgaccg aaaacattgt
     1861 tttacggtta aattcataac gagagacgac tagtcctggt cgtctttttt gaatttcttt
     1921 tcctacggag ggatcaaaat caatccatac aatatcacct tttttaggaa tataaattct
     1981 ttcttctgac actataaaat ttctctccct tcaggagtta aatcttccca ttcgtctttc
     2041 tcataatact cggcctcttc cccaccacta aatggatctt caatttttgg aactaacgta
     2101 attgttccat catcagaata cacaacgata tattcttggt tttctgaagg tttctttccg
     2161 ttatcagaag gtaaagttac cactacagaa cttccttgta atctagattt tgtaacaagc
     2221 attatcatca ctcccttaat aaaagtatac tatataacac taagtaacgc aaaattcccc
     2281 tttgtaaact atctagaaaa ttgttttgtt taaa
//
LOCUS       DAZKLD010000228         2245 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.233, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000228 DAZKLD010000000
VERSION     DAZKLD010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2245)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2245)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2245
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.233"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            82..1695
                     /locus_tag="ACG8KR_002880"
     CDS             82..1695
                     /locus_tag="ACG8KR_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFQ0202172.1"
                     /translation="MKVVVIGGVAGGPSFATRFRRLNEAHEIIIYERGENISYASCAL
                     PYYLGGVITDRDSLIERTPEILKTKNNIDVFTKHEVTAIDPSTKRLTVKDLSTNEEIK
                     TDYDKLIISSGARPDYPDIPGVFEAENGFVLRSVTDADRIKSFLEEKNPQHVVILGAG
                     VMGLELAENFKHRGLDVTLIDQLPQVAFPYDPEIANLVYDKLLKEGLAVHLETRVTEI
                     RDKGREIVLSDGSVLSADMLIFAVGVSPKNEVVKATGIQLSDTGQIIVDDQLQTNLPD
                     IYAIGDIIETTSVVTGQPIQSMLSSAANRQGHMLADILNGTPMRYRGYIGAGVAKIFD
                     HTASYAGMTEHELKASGITNYKTVFITPFDHAYFYPEATRLNLKLIFDADSGRILGGQ
                     AFGEKGVDKRMGELSVAITGNLTVFDLPDLELPYSPPYSTTRDPLNIAGYVAINQMTN
                     IVETIKASDIPENDLKEAFFLDIREPNKAPSGSISATKNIPMNELRDRINEIPKDKKV
                     YITFRRGLNTYTSARILAGLGIKAILIEE"
BASE COUNT          702 a          399 c          444 g          700 t
ORIGIN      
        1 taaacaataa ttttccggca caattatttg ctgtttttta ccataacata gtacgatcaa
       61 cgtagaaaag gagatgtttt tatgaaagtt gttgtcattg gcggtgtagc tggcggtcct
      121 tcatttgcca ctcgtttccg tcgattgaat gaagcgcacg aaatcattat ctatgaacgc
      181 ggagaaaata tttcttatgc aagttgtgct ttgccttatt atttaggagg tgtgatcact
      241 gaccgtgact cgctgatcga acgtacacca gaaatattga aaacaaaaaa caacatcgat
      301 gtctttacta aacacgaagt aacagcaatc gatccttcta ctaagcgatt aacagttaaa
      361 gacctatcca caaatgaaga aataaaaaca gattacgata agttgatcat ctcttctggt
      421 gctagaccag attatccgga tattcccgga gtttttgaag cagaaaacgg ctttgtcctc
      481 cgtagtgtga cggatgcgga tcgaatcaaa tcgttccttg aagaaaaaaa tccacaacat
      541 gtcgtcattc ttggtgcagg tgttatgggt ctggaattag ctgagaattt caagcatcgc
      601 ggcttagacg tgactttaat cgatcaattg ccacaagtcg ctttccctta tgatccagaa
      661 attgctaatt tagtttatga caaattgctg aaagaaggat tagccgttca tttagaaaca
      721 agagttactg agatccgtga taaaggtcga gaaatagtat tatcagatgg ctccgtcctt
      781 tctgctgata tgctaatttt tgctgttggt gtttctccga agaatgaagt ggtgaaagca
      841 accggcatac aattatcaga tacaggtcag atcattgttg atgaccagtt acaaaccaat
      901 ctgccggaca tctatgcgat tggcgatatt atcgaaacga ctagtgtagt gactggtcag
      961 ccgatccaaa gtatgctttc cagtgcggcc aatcgtcaag gacatatgct ggcagatatt
     1021 ttaaatggta cgcctatgcg ctatcgcgga tatattggtg caggtgtcgc aaaaatcttt
     1081 gatcatacag caagttatgc gggaatgaca gaacatgaat taaaagcatc aggcataaca
     1141 aattataaaa ctgtttttat cactcccttt gaccatgcct atttctatcc agaagctaca
     1201 agattaaatc taaagctgat ttttgatgca gatagcggtc gtattttagg tggacaagca
     1261 tttggagaaa aaggtgtcga taaacggatg ggagaacttt ctgtagcaat caccggaaac
     1321 ttgacagtct ttgatttgcc tgatttggag ttgccttact ctccaccgta ttctactacc
     1381 cgtgacccgt tgaatatagc tggttatgtc gcaatcaatc aaatgacgaa tatcgtagaa
     1441 acgatcaaag caagtgatat acccgaaaac gatttgaaag aagcgttctt tttagatata
     1501 cgtgaaccta ataaagcacc ttctggcagt atctcggcaa caaaaaatat tccaatgaat
     1561 gagttacgag atcgaatcaa tgaaatccca aaagataaaa aagtttacat tactttcaga
     1621 agaggattga atacttatac ttctgcccga atcttggcag gtttgggtat caaagcgatt
     1681 ttgattgaag aataaaaaaa cgaccgcggt gggtagccgc ggtctaggag ttatttttac
     1741 ttgaggagta aaaatgaaaa agtatatagg gttgttggtt ggtatgtata tagaatagaa
     1801 gataaatgtg aagaaattgt gcattatttc ttcacattta ttaggttata gtttaagaaa
     1861 gtttaaacat tgcgcaattt aattatttta gctgtttctt ttcataaaaa aacatgttgt
     1921 gactctgaga caaatcatag tccatagtga tgtctctaga gtaactacgc ctaatattac
     1981 gaggcatcat attaatctat cagcactagt acctaaacta gcatcccttt cgaatatttt
     2041 ttcttatata ataaagtttg ctttcactcc tctaatctca ccaaatcgac tggcttgcgt
     2101 tgtgtgatac taggaactga ttggtttaga acgacttttc tattgtaatc atatttttcg
     2161 ccatcctcgt tgtcttctaa tccttcaatg aatgatccct aatgtgtgta ttaaataatt
     2221 taattttttt gttcctattt attta
//
LOCUS       DAZKLD010000229         2222 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.234, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000229 DAZKLD010000000
VERSION     DAZKLD010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2222)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2222)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2222
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.234"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(89..1174)
                     /gene="xerS"
                     /locus_tag="ACG8KR_002881"
     CDS             complement(89..1174)
                     /gene="xerS"
                     /locus_tag="ACG8KR_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HFQ0202173.1"
                     /translation="MKNTEIYQKIDTIILHMPDYIKDFVQDREDKDQSPRTTLEYLKN
                     YKLFYEWLLSESIVPDTSITSIRQLTAYDLNLYKSHLKRRTKENVKKETTKTKLKDNN
                     NLGLSTSTINRNITALKVLFKYLSKSSNNPLGKPYLENNPMDQVATITDKTTLAARAN
                     AIEKKLFLDEDTQNYLDYIANEYKKTLSKRALIYYQRDVERDLAINALILGSGLRLSE
                     VVNINLEDLSLEKNNVVVTRKGNKRDAVNIAAFAMEYLANYLAIRKERYKVTENEKAL
                     FLAIYQGEAKRISGIAIERMVAKYSKGFRVQVSPHKLRHTLATRLYQQTNSLVLTAQQ
                     LGHTSTNTTTLYTHIDNAATIDALNAL"
     gene            1523..1718
                     /locus_tag="ACG8KR_002882"
                     /pseudo
     CDS             1523..1718
                     /locus_tag="ACG8KR_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290351.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module antitoxin"
BASE COUNT          737 a          320 c          363 g          802 t
ORIGIN      
        1 taaaaatgat aatgaagctt gcctttaaag ttgggctttt atctaatttt agagacaggc
       61 ttcattatta tactattttt tactacattt acaaagcgtt caaagcatca attgtagctg
      121 cattgtctat atgagtatat aaagttgttg tatttgtact tgtatgtccc aattgttggg
      181 cagttaatac caatgaattt gtctgttgat agagtctagt agccaaagta tgacgtaatt
      241 tatgtggact gacttggact ctgaatcctt ttgaatattt tgctaccatc cgttcgattg
      301 caataccgga tatacgcttg gcttctcctt gataaattgc tagaaataaa gctttttcgt
      361 tttctgttac tttgtatcgt tctttccgaa tagctagata attagctaaa tattccattg
      421 caaatgctgc tatatttact gcgtctctct tatttccttt tctagttact accacattat
      481 tcttttctaa agataggtcc tctaaattaa tatttacaac ctcggagagt cttaatccag
      541 atcctaaaat caatgcattg atcgccaggt ctctttctac atctcgttga taatagatca
      601 aagctcgttt gctcaaagtt tttttatatt cattggcaat ataatctaga taattttgtg
      661 tatcttcgtc tagaaataat tttttctcga ttgcgtttgc acgggcagct aacgtagttt
      721 tatctgtgat agttgctact tgatccatag gattattttc taagtatggt tttcctaaag
      781 gattattgga acttttcgaa agatatttaa atagaacctt taatgctgtg atatttctat
      841 tgatagtaga agtagataga cctaagttat tattatcttt gagcttagtt tttgttgtct
      901 ctttttttac attttccttt gtgcgacgtt tcaaatgaga tttgtacaag tttagatcat
      961 aggcagttaa ctgtcttatc gaggtgattg aagtatcggg aacgatagac tcactcagaa
     1021 gccattcata aaagagctta taatttttta gatattctaa agtagttcga ggtgattgat
     1081 ctttatcctc acgatcttga acaaaatctt ttatataatc gggcatatga agtatgattg
     1141 tatctatttt ttgatatatt tctgtatttt tcatgttatt catcctttcg ataagtaggg
     1201 gagaggagta tctctataat tggactatac caattataga gtgttttgtc aattggtata
     1261 ctctgaaatt tattaattta tttcaaaaaa gcagataaat aggtatagat ttaataattt
     1321 aaaataaatg gtatacatga ataggaaagg aaacagaagg aggcaatctt aattttttaa
     1381 attgtctaaa ttatatagaa agaacaatct ttctatataa ttagacataa gagagactac
     1441 ggttgatttg atattaacaa gcgtatatga atgtatatac aaataaaaaa ggggagggag
     1501 aagttaaaga aaattcagag aagtggggca ctcagtagta ttaacaattt aaaaggaatt
     1561 tcttaaagaa aatgggttgc atcctggaga tacaattttt ttagataaag aaaaaattca
     1621 agcaacaatt caaaaaaatc ttaactgctg agattgatgt agcaatatct caatcatcaa
     1681 caaagtacga tcctgccttt caaaagctta ttgataaatg tttattcatt ataaaaataa
     1741 aagaaaaatt tatgtcagct tttttcatca aagtggtaat gataattcgt ctattaattg
     1801 tattttttag gaaatatggt ataattgttt taacgaaaag gtaattcatg atacaccaaa
     1861 aaccactagc tggcgggcta gtggttttct tttacgttct attcagatta acgggtaggc
     1921 ttactgtctt ttttggcaat aattttgcaa tcaagccatt tacagatgta atgcgcggat
     1981 acttctgcta agattgcaaa aaacatatcg aaaaggtaat tcataataca acctcctccc
     2041 cttgccataa taggccgggg tagcagacag cataaatatt ataccataac tgaaaatttc
     2101 attttagtac atttcaatat attgatgaac cagcacaata aaacatgcca tgactaacag
     2161 aaaaaaactt attaaaccag atataacttg taaacatcca actatataga aaacgtaaat
     2221 tt
//
LOCUS       DAZKLD010000230         2205 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.235, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000230 DAZKLD010000000
VERSION     DAZKLD010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2205)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2205)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2205
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.235"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(147..>2205)
                     /locus_tag="ACG8KR_002883"
     CDS             complement(147..>2205)
                     /locus_tag="ACG8KR_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="peptidoglycan D,D-transpeptidase FtsI family
                     protein"
                     /protein_id="HFQ0202174.1"
                     /translation="SHVPFRLNFLFFIIFTLFVALIARLGYLQIVNGEEMEARVKSTS
                     TITVQESTPRGMIYDSTGKALVTNKANQAITYTRGTQVTAKDLLEIATKLNSLIDVPV
                     DENLTDRDKKDFWLADPKHLKEATKRLSAKEKQLGNSEQYSAMVNKVTTDEINLNEEQ
                     LKIATIFKRMNSAYELNTVYIKNSGVTDQELAVVAERASELPGVSTGTDWTREYNAAS
                     SLKSILGSVTTEKQGLPADEAEKYLAKGYSRNDRVGQSYLEKQYEDVLQGTKTQYEVS
                     LDNEGNVSNQKEIFSGEKGSNLMLSMNAEFQSKVEEILKRNYQTLINNGKAQYSPGAY
                     AVAMNPQTGEVLAMTGFSHEQGSKEITENALGTITSAFDPGSVVKAGTLTAGWASNAI
                     SGNQVLIDEPIRLQGASEKSSVFNRSGQVALDAVKASELSSNTYMIKVALKMLGLDYT
                     PGMGLPSLDEEAKAYQQLRDSFKEFGLGTTTGIDLPNESPGISRSVDYMKKFNADNGK
                     EWYAPGNFTDLAFGQFDTYTPIQLAQYASTVANGGKRVQPHLVKAIYGNDENGNLGEV
                     KKEIGTTVENTVNISAENMAILREGFHQVVHGTDPYTTAKPLASAKMDLSAKTGTAET
                     VAEGHPDIATVNSNIVAYGPTDNPEIAISVVLPNLLDEQDHINLTIAKEIMDAYYDMF
                     MAK"
BASE COUNT          566 a          494 c          402 g          743 t
ORIGIN      
        1 tttgtcacaa ccttttttgc cgagtaaacg gtgttcaaaa gccagtttct cggtatgaag
       61 tcaaatttaa gcaaaaatgt gagaagctat tttcacaaaa tttttcttca tactcgaaac
      121 taagcggctt tttcacgacc tcttttttat tttgccatga acatatcata atacgcgtcc
      181 attatttctt tagcaatcgt caagtttatg tggtcttgtt cgtctaataa gttcggcaag
      241 acaacactga tggcaatttc cggattgtct gttggtccgt atgccacgat attactattg
      301 actgtcgcaa tatctggatg accttccgca accgtttctg cagtccctgt ttttgcggaa
      361 aggtccattt tcgcactggc taatggtttg gctgttgtgt aaggatcggt accatggaca
      421 acttggtgga atccttcacg gaggatcgcc atattttctg cagaaatatt gaccgtattt
      481 tctactgttg tgccaatttc tttcttcact tcacccaagt ttccattttc gtcattgcca
      541 tagatcgctt tgactaggtg cggctgtact cgtttcccgc cattagcgac tgttgaagca
      601 tattgagcta attggattgg tgtatatgtg tcaaactgtc caaatgccaa atcggtaaag
      661 ttacctggag cataccattc tttgccatta tccgcattga acttcttcat ataatcaaca
      721 gaacgtgaga tcccaggaga ttcattcggc aaatcgatcc cagtcgttgt accaagacca
      781 aactctttga aagagtctct caattgctga taagctttag cttcttcatc taaactaggt
      841 agtcccattc caggtgtata gtctagtcct aacatcttta acgccacttt gatcatatac
      901 gtattagaag acaattccga tgctttgact gcatcaagag caacttgtcc gcttcgatta
      961 aagacagagc ttttttctga agcaccttgc agacgaatcg gctcatcaat caaaacttga
     1021 tttccgctga tcgcgttact tgcccagcct gctgtcaaag taccagcctt gactacagac
     1081 cctggatcaa aggcgctagt aatcgtgcct aaagcattct cggtgatttc tttggaacct
     1141 tgttcatgac tgaaaccagt cattgctagc acttctcctg tctgaggatt catagccact
     1201 gcataagcac caggcgaata ctgtgctttg ccgttgttga tcaatgtttg ataattcctt
     1261 ttcaaaattt cttctacttt tgattggaat tctgcattca tagataacat taaattagaa
     1321 cctttttccc cagagaagat ttctttttga ttcgaaacat ttccttcatt atcaagggaa
     1381 acttcatatt gggttttggt tccttgcagg acatcttcat attgtttttc caaataactt
     1441 tgtcctacac ggtcattccg agaataacct tttgctaggt atttctctgc ttcatctgct
     1501 ggaagtcctt gcttttcagt cgtcacactg ccaagaatac ttttcagtga acttgctgca
     1561 ttatattctc gtgtccagtc tgtgccagtc gaaactcctg gaagttcaga agcgcgttct
     1621 gccacaacag ctaactcttg atcagtaaca ccagagtttt tgatatagac agtgtttaat
     1681 tcatatgcac tattcatccg tttaaagatg gtagcaattt ttaattgctc ctcgtttaaa
     1741 ttaatctcat cagttgtgac tttattgacc atcgcactat attgttcgct gttccctaat
     1801 tgcttttctt ttgccgaaag tctttttgtg gcttctttca aatgtttggg atcagccagc
     1861 caaaaatctt ttttatctct atctgtcaga ttttcatcta ccggaacatc gatcagcgag
     1921 ttcagcttcg ttgcgatttc aagcaaatct ttagccgtaa cttgtgttcc tctcgtatat
     1981 gtgatggcct gattcgcttt attcgtaact aaagcttttc ctgtggagtc gtagatcatt
     2041 ccgcgaggag tactttcttg gaccgtaatc gtagaagtag acttcactcg cgcctccatc
     2101 tcttcgccgt tcacgatctg caaataaccc aatctagcaa tcaacgcaac aaataacgta
     2161 aatataatga aaaaaagaaa atttaagcga aaaggaacat gggac
//
LOCUS       DAZKLD010000231         2196 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.236, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000231 DAZKLD010000000
VERSION     DAZKLD010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2196)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2196)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2196
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.236"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(552..2018)
                     /locus_tag="ACG8KR_002884"
     CDS             complement(552..2018)
                     /locus_tag="ACG8KR_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFQ0202175.1"
                     /translation="MNNKQLRWFTVALIAFNMVWGMGNVVNNYAQQGVSVVTSWLLIL
                     AIYFIPYALIVGQLGSAFKDSKGGVSSWVENTTSNKRLAYYAAWTYWVVHIPYLAQKP
                     QAILIAGGWAVQGNGNIVNTMSVQMVAVISLIIFLAFLYLSTKGLSTLKVIGGLAGTA
                     MFVMSILFILLAVTAPFINPTMEFATPHMNQVKTYIPTFDFSYFTTVSMLVFAVGGAE
                     KISPYVNSTRNPAKEFPKGMIFLAAMVGICAVLGSVAMGMLFASDNIPKDLMANGAYS
                     AFQILGNYYGIGNSLMIIYALTNMIGQISALAFSIDAPLKILLADADPEFVPNWLRKR
                     NRKGTLVNGYLLTGILVGIIILLPIFGIKDMNELVKWLTNLNSVVMPMRYLWVFLAYM
                     MLNKAYKKFNSEYKFVKNPKIGFIFGAWCFAFTAFACILGMVPKVDYTADPKAWWFQL
                     FTNIITPIVLILLGMILPAIARRDKNKEITEPETVVQP"
BASE COUNT          682 a          447 c          375 g          692 t
ORIGIN      
        1 caagggactg tgacaaaagt cgtgtcaaag tcccttgttt cgaataaatg gtgttcaatc
       61 agctgacctt cagcattaac tcaaaaaacg gcaaaatatg aggagctatt ttttcgtttg
      121 ttcctctaat actcaggtca aatcgctgat tttacaacct cttccttaaa cggtgttctc
      181 ctgccaacac ctcggtcaca aggcacaata gtcaacaaac atgagaagtt gttttttgaa
      241 attgctcctt gtgactcgga gttaaacggc aggctcacta cctctttctt aaacggtgtt
      301 caaaagcttg catctgcggt aataagccca gcaaaacgaa aaatatgagg ggctattttt
      361 gtttggctgt tcttattact tgatgcagga cagctttttc acaacctctg attcacggtg
      421 ttccagaagt agcttctgcg aaaaacaagc cgaaacttca caaaaatttg agaagcaatt
      481 ttcgtaaatt ccttcttatt tcttgaagct gaccacttct gtcacaacct cttgtaacaa
      541 gtatttctat tttaaggctg aaccactgtt tctggttcag tgatttcttt gtttttgtct
      601 cgacgagcaa ttgctggcaa gatcatacct aataagatca agacaattgg tgtgatgata
      661 ttcgtgaata attggaacca ccatgcttta ggatcagcag tataatctac ttttggcacc
      721 attcctagga tacatgcaaa cgcagtgaag gcaaagcacc aagcaccaaa gataaaaccg
      781 atttttggat ttttaacaaa tttatattca gaattgaatt tcttataagc tttattcaac
      841 atcatatatg ccaagaacac ccataggtaa cgcattggca tgacaactga gttcaagttc
      901 gtcaaccatt ttactaattc attcatatct ttgataccaa agatcggcaa caaaataatg
      961 attcctacta gtatacctgt cagaagataa ccattcacca atgtaccttt acgattacgt
     1021 ttacgtagcc agtttggtac aaattctgga tccgcatctg ctaacaagat ttttagtggt
     1081 gcatcaattg aaaaggctaa tgcggagatt tgtccaatca tgtttgtcaa tgcgtaaatg
     1141 atcatcaatg agttaccgat accatagtaa ttacctaaaa tttgaaaagc actgtatgcc
     1201 ccatttgcca tcaagtcttt aggaatatta tcgctagcaa acagcatacc cattgcgact
     1261 gagcctaata cagcacaaat ccctaccatt gctgctaaga agatcatccc tttaggaaat
     1321 tcctttgcag gatttcttgt agagttgaca taaggggata ttttttccgc tcctccaact
     1381 gcaaatacta gcatcgaaac ggttgtgaaa taactgaaat caaatgttgg aatatatgtt
     1441 ttcacttgat tcatatgtgg tgtggcaaat tccatcgttg gattgatgaa cggtgccgtt
     1501 acagctaaaa gaataaataa gatcgacatg acgaacatcg ctgttccggc cagtccgcca
     1561 atcactttca aagtagaaag tccttttgtt gataaatata aaaaggcaag aaatataata
     1621 agtgaaataa ctgctaccat ttgtactgac atcgtattta cgatatttcc atttccttgg
     1681 actgcccaac cgccagcaat caggattgct tgaggctttt gtgccaaata aggaatatgg
     1741 acgacccagt aagtccaagc ggcatagtat gccagtcgtt tattagatgt cgtattttcg
     1801 acccatgaac tgacgcctcc tttactatcc ttgaaggctg accctaattg tccaacgatc
     1861 aatgcgtatg gaataaaata aatggccaaa atcaatagcc aagaggtgac aactgatact
     1921 ccttgctgag catagttatt aactacattc cccatgcccc aaaccatgtt aaatgcaatt
     1981 aacgcaacag taaaccagcg taattgtttg ttgttcattc gttctctcct ttatgataaa
     2041 aatttgttat ccagatgtaa tcatattcaa tttttaacaa acttacaaga cagtgttatt
     2101 ttatgtgtgt attcttttat ttaatacaca tcgttttttc atattttttc catcaaaaaa
     2161 ataagttatt ttcttttatt tcatttacct tattga
//
LOCUS       DAZKLD010000232         2076 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.237, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000232 DAZKLD010000000
VERSION     DAZKLD010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2076)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2076)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2076
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.237"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..425)
                     /locus_tag="ACG8KR_002885"
     CDS             complement(<1..425)
                     /locus_tag="ACG8KR_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HFQ0202176.1"
                     /translation="MMETITYSPLNLFTNFQEAAANFSETPIIFDEPLSAFPELGLET
                     TYKKCCESVLKRAYQLAHLGVKSGDKVIIYKSSAFDTYLLAVSVSYLGAVPVMTSYHL
                     PTTTMEVFIDRLEDPFILFDDETKKRVREISNGTKSKQIP"
     gene            complement(534..1565)
                     /locus_tag="ACG8KR_002886"
     CDS             complement(534..1565)
                     /locus_tag="ACG8KR_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFQ0202177.1"
                     /translation="MDSKTRKQLQEKLKQRTHLLPSVFQQFLEENRSSLSLGSRYEYA
                     KDFHLFTDYLRKVFSEEVDDQLIIHLEKQDYQNFLAKIYRHTRSFQTTANQETEQLFE
                     NSYYGISRKQYSLNHFLRFLYQKGLIEEEISLLGTSLPETTKAAPRYIDAELFKDFEH
                     LFIPIEGFQTSREASFEEKNRPRNLAITAILLYCGLKIHEVVELKRKDVSLTKQILNL
                     NRKENRLNKVPIPKEAMPFLENYFKLTAQNTDENSFVFRSSHDQQISPRTIRQILSKI
                     TVYKNVSPELCRKTYRYYAELYLDDADAVNYLCGNRYLSSHSFQDIWEKMVDFSYHEL
                     AAFVRVSSI"
BASE COUNT          642 a          336 c          360 g          738 t
ORIGIN      
        1 ggaatctgtt ttgattttgt tccatttgaa atttctctca ctcgcttttt tgtctcatca
       61 tcaaataaaa tgaaagggtc ttctaaacga tcgataaata cttccattgt tgttgttggt
      121 agatgataag aagtcatgac aggcactgca ccaaggtaag acacagaaac agctagaagg
      181 tatgtgtcaa aagcggaact cttataaata ataactttgt ctccggattt cactccaaga
      241 tgagccaatt gataagcccg tttcaacaca ctttcgcagc attttttgta agttgtttca
      301 agtcctaatt ctgggaaagc tgaaagtggc tcatcaaaga taatcggggt ctcggaaaag
      361 ttagctgcag cttcttgaaa attcgtaaat aagttcaaag gggaatacgt tatggtctcc
      421 atcattcaca ctccattatc ttttgttaag taatatacaa attcaagata atcgtaacat
      481 aatcctttat ataataacat agtttattgt gaaaaaaaga tcatacattt gttttatatg
      541 gaagaaaccc gaacgaatgc cgctaattcg tgataagaaa aatcaaccat cttttcccag
      601 atatcttgaa aagaatggga tgagaggtat cgattaccac ataaataatt taccgcgtca
      661 gcatcatcta aatatagttc tgcataataa cgatatgttt ttctgcatag ttctggcgat
      721 acgtttttat atactgtaat tttgctcaaa atttggcgaa tagtccgagg agaaatttgc
      781 tgatcatgtg aggaacgaaa aacaaaagaa ttttcgtccg tattttgtgc tgttaactta
      841 aaatagttct ccagaaatgg catcgcttct tttggtattg gtactttatt cagtctgttt
      901 tcttttctat tcagattaag aatctgcttg gtcaaagata catctttacg tttaagttct
      961 actacttcat ggatctttaa tccgcaatac agaagaatgg cagtaattgc aaggttcctc
     1021 ggtctattct tttcttcaaa gctagcttct ctgcttgttt gaaagccttc aatcgggata
     1081 aacaaatgct caaaatcttt gaaaagttct gcatcgatat atcgcggagc tgctttggtt
     1141 gtttccggta aacttgtacc caaaagagaa atctcttctt caatcagtcc tttttgatac
     1201 aaaaatcgta aaaaatgatt taatgaatat tgcttccttg aaatcccgta atagctattt
     1261 tcaaacaatt gctccgtttc ttggttcgct gtagtttgga acgaacgagt atgtcgatag
     1321 attttagcta agaaattctg ataatcttgt ttttctaaat ggataatcag ctgatcatcc
     1381 acttcttcag aaaatacttt tctcaaataa tcagtaaaca gatgaaaatc ttttgcgtat
     1441 tcatagcgac tgcctaatga aagggaagaa cgattttctt ctaaaaactg ttgaaagacg
     1501 gaagggagca agtgtgtacg ttgctttaac ttttcttgca gttgttttct agttttgctg
     1561 tccattacaa attccctcca atagtgttca ttttctatat tttaccgcat ttcatcaaat
     1621 gaagatagac agtttgtctt gatcttagga agcaaatttt taactaagta ctagctaaaa
     1681 gatatgaagt aagaagatac atttttagtt attttattag tgcagtcatt cttttgatct
     1741 tttaattgat ttatcctctt tttagctatg tggttacttc atgagcaatg aaatggattt
     1801 ggttcgttct aatggcagga ggctctttct tttatttccg aaaaattaga aaacattttt
     1861 tcagaatcga ttggctctcc atttattcaa taatggtttg tctaacgata ttgttgggat
     1921 aattcttaaa gaagtattag aaatagttgg tacttcatta ctttacatga agatttattc
     1981 tatggtagga gcaattatat tgagaattaa tcctttttta tataagttgt ttgcttattg
     2041 atggattgga taaattaaac catcacaacg agtgga
//
LOCUS       DAZKLD010000233         2054 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.238, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000233 DAZKLD010000000
VERSION     DAZKLD010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2054)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2054)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2054
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.238"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(366..1658)
                     /locus_tag="ACG8KR_002887"
     CDS             complement(366..1658)
                     /locus_tag="ACG8KR_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HFQ0202178.1"
                     /translation="MARKKRNPDAEKLAESILNAYQPESVDDMQDALKDVFGPLFEKM
                     LQGELNNHLGYDAHSKEPKEHDNRRNGYGTKTLKTSFGEVAIDVPRDREASFEPELIP
                     KRKRDVSDIEGKVLSMYARGMSQRDIAATVEAIYGFDISHEMISDITDAVLPELEEWQ
                     ARPLAKCYAFLFVDCMYVTLRENYEAKEYAVYTILGYDLKGNKEILGLWLNQTESKNR
                     WMQVFDELKARGVEDVFFISMDGVSGLEEGAKAIFPSVIVQRCIVHLVRNALRYIPSK
                     DYKEVCRDMKKFYGASSLNAAHAAFDSFQNRWSHYSGAVDVWKRNFAHVEQLFDYGSA
                     IRKIMYTTNAVESVHSSFRKVTKKGAFSNENALLKLLYLRTKELHAKWSGGRIQNWAM
                     VLNQLMINETFSSRMKSMRFTFRNNGRFIQLCVRSKFI"
BASE COUNT          663 a          396 c          330 g          665 t
ORIGIN      
        1 gtatgggcta ctctgtgata cttattagcc attaaactat tttacccaca tagcatatat
       61 tttgaaatcg ttcattccgt aaattcaact aaatatgcct aaaaaaataa aaaatctaag
      121 atagaataag atttattcag tcttagatgg atttatagca actttttcgt aacaacttta
      181 aaaaatacga aaagaaagag ctgttttact ctgactacgt tgctaaattg tcgtttaatc
      241 attcatttaa accaaatctt tttttataga attcgtttta atcctcctga atccaaaaac
      301 tatgaaaacc gtaattaaat agttaagagt ttttaaccac cataattctc tcggctattt
      361 tgaaatcaga taaattttga tcggacacat agttgaataa acctaccgtt gttacggaag
      421 gtaaatcgca tacttttcat tctagaggag aaggtctcgt tgatcattaa ctgattaagc
      481 accatagccc agttttgaat tcgaccacct gaccacttcg cgtgtaattc tttcgtgcgt
      541 aaataaagta gttttaggag tgcattctca ttagagaacg ctcctttttt tgtgactttt
      601 ctgaagctgg agtggacact ttcaaccgca ttggtagtgt acataatttt tcgaatggca
      661 ctaccataat caaatagttg ttcaacatgt gcaaagttcc gtttccagac atctacagca
      721 ccagaataat gagaccaccg attttgaaaa ctgtcaaaag cagcatgtgc agcgtttaga
      781 gaagaagcac cgtagaactt tttcatatct cggcagactt ccttatagtc cttacttgga
      841 atatagcgca atgcatttcg aacaagatga acaatacagc gctgaacaat taccgatgga
      901 aagatcgctt ttgcgccttc ttcgaggcca gaaacaccgt ccatcgaaat gaaaaagaca
      961 tcttcgacac cacgtgcttt cagttcatca aatacttgca tccagcgatt tttagattct
     1021 gtttgattta accataatcc taaaatctcc ttatttcctt tgagatcata gccaagaatg
     1081 gtgtatacag catattcttt ggcttcataa ttttctcgta aagtaacata catacaatca
     1141 acgaatagaa aggcataaca ctttgctagg gggcgggctt gccattcttc caattcagga
     1201 aggacagcgt cagtgatatc tgaaatcatt tcatgggaaa tatcaaagcc ataaatagct
     1261 tcgacggttg cggcaatatc tcgttgactc attcctcgtg catacatgga aagaaccttc
     1321 ccttcgatgt cggagacatc tcgttttctc ttaggaatta actctggttc aaaggaagct
     1381 tcccggtctc taggaacatc aatagctact tcaccaaaac tggttttaag cgttttagtt
     1441 ccatagccat ttcgacggtt atcgtgttcc ttaggctctt tagaatgggc atcataacct
     1501 aaatgattat tcaattctcc ttgaagcatt ttttcaaaaa ggggcccaaa cacatctttc
     1561 aaagcatctt gcatgtcatc gacagattca ggttgatagg cattcagaat ggattcagct
     1621 aacttttcgg catcaggatt tcttttcttt ctagccatcg tgtaatcacc tcttgatctt
     1681 attgtagaaa aagaaaaacc gcgaagcaac cacctgccta ggattaatgg ttacttacac
     1741 ggtttatatt acactctcat cattaaaaaa gtatctttcg atattactaa acaatgaaat
     1801 tatctatttg aaatttaaat ctgataatat attgagtgtt ccaatgaggg ctttgatcct
     1861 attatccata tctttttcag ttttaatatt gatacacatt ataagaaaaa gaaacttttg
     1921 gttaatttat ttcatcccag tgcaaaatat aaaaaagtaa aatagaccat aggcaataga
     1981 ttaacaatta atataataca agcaaatatt ctattcattg tatttctgct ttcaaaaagt
     2041 tctaatattg aaag
//
LOCUS       DAZKLD010000234         2030 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.239, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000234 DAZKLD010000000
VERSION     DAZKLD010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2030)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2030)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2030
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.239"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(60..302)
                     /locus_tag="ACG8KR_002888"
     CDS             complement(60..302)
                     /locus_tag="ACG8KR_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFQ0202179.1"
                     /translation="MGFIWALIVGGIIGAIAGAITNKGSSMGIIANIIAGLVGSAIGQ
                     ALLGTWGPTIAGMAIIPSLIGAIILVLVVSFFARKL"
     gene            complement(334..891)
                     /locus_tag="ACG8KR_002889"
     CDS             complement(334..891)
                     /locus_tag="ACG8KR_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFQ0202180.1"
                     /translation="MENKNMNTTNGSVLANEKGENRMMNGELTYEDKVIQKIIGYALK
                     DVDGLLTLDGGFFSNMAEKLVNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYN
                     QIKELISTEVNNMTHLDVIEVNVNVADIKTQEEYQKDQETVQDKVTEAAKSTGQFASR
                     QTDKAKSAVGKGAQKVKENNEPRVQ"
     gene            complement(904..1092)
                     /locus_tag="ACG8KR_002890"
     CDS             complement(904..1092)
                     /locus_tag="ACG8KR_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HFQ0202181.1"
                     /translation="MKELFSAYKLPIIFGGLGLLLAVLWLTIGFFKTLLIIIMTAIGA
                     GIGFYLKQSGIVDQFFKS"
     gene            complement(1105..1671)
                     /gene="amaP"
                     /locus_tag="ACG8KR_002891"
     CDS             complement(1105..1671)
                     /gene="amaP"
                     /locus_tag="ACG8KR_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HFQ0202182.1"
                     /translation="MNKGLKIIGVIMSLLLLSVFLAIALINAPYIMPEQLERFRFFTL
                     TNYYMQQYIFWAAVVFAVLAIIILLVVLFYPKSRGTFVMKREDGKLTIDKKAIEGLVR
                     SHLHEEEFIHSPKVRIRSTKNRIHIHVNGDLKRTSSLVGKTGVLMQDIEEEVTKVLGT
                     KETVKVAVTYSGYEEQEDTRDYKHSRVE"
BASE COUNT          558 a          408 c          307 g          757 t
ORIGIN      
        1 gtgtcgctac ggttctcaaa tttgttcttc atttcaacat aagtttttct tcaatttgct
       61 tataatttac gtgcaaagaa tgaaacgact aatactaaaa tgattgcacc gatcaatgat
      121 ggaatgatag ccattcctgc aattgttggt ccccaagttc ctagtaaagc ttgtccaatt
      181 gctgaaccta ctaaaccagc aatgatgttt gcgatgatcc ccattgatga tcctttattc
      241 gtgattgctc ctgcaattgc tccaatgata ccgccgacga ttaatgccca aataaatccc
      301 attttaaatt cctcctaact gacttcttac ttcttattgt acacgtggtt cgttattttc
      361 tttgactttt tgtgcacctt tgcctacagc agattttgct ttatctgttt ggcgtgaagc
      421 aaattgtcct gtggattttg ctgcttctgt tactttgtct tgtactgttt cttgatcttt
      481 ttggtattct tcttgtgttt tgatgtctgc aacattcaca ttcacttcga taacatctaa
      541 atgtgtcata ttgtttactt cagtactgat caattctttg atttggttgt aaatatcttc
      601 gatgtcttta ccgtattcta cgatcacatc tagatccaca gctacttgtt tcttacctac
      661 ttctgtattg atacctgctg ttacgttatc tgtgttgact aatttttcag ccatatttga
      721 gaaaaatccg ccatctaatg tcaatagacc atctacatct ttcaatgcat aaccgatgat
      781 tttttggatg actttgtctt cataagtcaa ttcaccattc atcatgcggt tttctccttt
      841 ttcatttgcc aaaactgatc catttgttgt attcatgttt ttgttttcca tctttgtttc
      901 ctcctatgat ttaaaaaatt gatcaactat tcctgactgt ttcaaataaa aaccgattcc
      961 tgcgccaatt gctgtcatga ttatgatcaa caatgtcttg aaaaatccaa tcgttaacca
     1021 taaaacagct agcagtagtc caagaccccc aaagataata gggagcttgt atgctgaaaa
     1081 tagttctttc attatttggc cctcctattc cactcgagag tgcttgtagt ctcttgtatc
     1141 ttcttgttct tcataaccgc tgtatgtgac tgccactttg accgtttctt ttgttccgag
     1201 tactttagtt acttcttctt cgatatcttg catcaataca cctgttttcc ctactaatga
     1261 agaagttcgt ttcaagtctc cgttgacatg gatatggatt cgatttttcg ttgaacgtat
     1321 ccgtacttta ggtgaatgaa tgaactcttc ttcgtgtaaa tgggaacgta ccaacccttc
     1381 gatcgctttt ttgtcgattg ttagtttgcc atcttcccgt ttcataacga aagttcctct
     1441 tgactttgga tagaaaagaa cgactagcaa gatgataata gcaagaacgg caaatactac
     1501 agctgcccag aaaatatatt gttgcatgta gtaattcgtt aaagtaaaga agcgaaatcg
     1561 ttccaattgt tcaggcataa tatacggcgc attgatcaac gcaatcgcta gaaatactga
     1621 aagtagcagt aaactcatga taacaccgat tatttttaac cccttattca ttttccacct
     1681 cctataaact tatttttacc ctgtaaataa ctctaccgac aatcacagta tagatagcga
     1741 acagttagca tagcatcagt aaataaagcg ctaccaaaca ttgctggatc ataacggcgt
     1801 tttattgcca gtctgatact gaaataacaa ttgcttactt attctctatt tacttttgat
     1861 tgctggctat acttaaagga tattattcaa gattaaaaat tgcaaagatt atgctcagtt
     1921 attattacaa gattctattt atttattaaa ttttttcgga gaattaaaaa atacaataaa
     1981 taactttagt tactttaatg cgttcttact ttatttttat attatcattt
//
LOCUS       DAZKLD010000235         2023 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.240, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000235 DAZKLD010000000
VERSION     DAZKLD010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2023)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2023)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2023
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.240"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            142..744
                     /locus_tag="ACG8KR_002892"
     CDS             142..744
                     /locus_tag="ACG8KR_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbiJ-NTD4 domain-containing protein"
                     /protein_id="HFQ0202183.1"
                     /translation="MEDIILRDRLTDPLKNSLANVIYDILREADKVYSNYNGKVSWVI
                     YSFLIKEFFKEQINNSYELGYHFTVYYEDIIKRLEDLEWYAYYDLLEVFASAKILDKK
                     KREEVNKILESENSGYRLSESNEFIPITDDISIKSINAVSESPFNYAKNHIHKSLTYI
                     SEKENQDYNTVVREAISAVESCLIEFSGLPANKKILWELL"
     gene            1099..1335
                     /locus_tag="ACG8KR_002893"
     CDS             1099..1335
                     /locus_tag="ACG8KR_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HFQ0202184.1"
                     /translation="MNIKKRKIREVGNSIVVTLSKESLLQKGLKPGDTIFIDEDKMME
                     AIVKEESKIDLEIDMYVNQALSEYDAAFKELVEK"
     gene            1332..1745
                     /locus_tag="ACG8KR_002894"
     CDS             1332..1745
                     /locus_tag="ACG8KR_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HFQ0202185.1"
                     /translation="MIYLSEKDIVKLNVIQIKKYSPRETIGVIDKNALSMAVNQPKQH
                     IFGKDLYPDKFSKAAVLLINLIKKHPFHNANKRTAFLAAYVFLKLNGYSLKMKNQEVV
                     EFVVEIATYQGDFDDLKEATTDILKKNSTKTDSKK"
BASE COUNT          779 a          245 c          346 g          653 t
ORIGIN      
        1 ccattaaaca atcttaacag atatttcatt ttatttatgt aaaacaacac gaataattat
       61 tgcttcgtaa agttatatga gtataagtta atttactgga ggtagtgcaa tgcttttttc
      121 tgaaagattt ggttttaaaa aatggaggat atcatactga gagatagatt gacagaccct
      181 cttaaaaata gtttagcaaa tgtgatttat gatatattac gagaggctga taaagtttat
      241 agtaattata atggaaaagt ctcatgggta atatattctt ttttaattaa agagtttttt
      301 aaagaacaga taaataatag ctatgagttg ggttatcatt ttactgttta ttatgaagat
      361 attatcaaga ggttagaaga cttggagtgg tatgcttatt atgatttatt agaggttttt
      421 gcaagtgcta aaatcttgga taaaaagaaa agagaggaag taaataaaat tctggagtcg
      481 gaaaattctg gttacagatt aagcgaaagc aatgaattta ttccaatcac agatgatatt
      541 tctattaagt ctataaatgc tgtatctgaa tcaccattta attacgcaaa aaatcatatc
      601 cataaatctc ttacatatat aagcgagaag gaaaatcaag attataatac tgtagtgaga
      661 gaagcaatta gtgctgtcga aagttgtctt atcgaatttt caggattacc agctaataaa
      721 aaaatacttt gggaactgct ataaaagcaa ttaaagacaa atcgaataac aatgtcgatc
      781 ttacattttt aaaaccattt gagacaatgt atggtttggc gagtaataat ggaatacgac
      841 atgcagaaaa tgaaaaaaca attgtctcag atttacctga tgcagttttg gtgttaacaa
      901 catgttcagc cgtaattaat tatttgggag taaaaatagc atatgactcc aataaatcat
      961 gaaaagttaa aattcatcca agagctacat tataactatt aatttccaac tctttaaaag
     1021 tttagtaatt gaaggaagta ataagggtga ttgaacaata cggatatacg gtgtatactt
     1081 atcgtaggag ttgatgatat gaatattaaa aaacgcaaaa tcagagaagt aggtaattca
     1141 atagttgtaa ctttgtcaaa agagagttta ttgcaaaaag gtttgaaacc tggagatact
     1201 atttttattg atgaagataa aatgatggaa gctattgtaa aagaggaaag caagatagat
     1261 cttgaaattg atatgtatgt taaccaagct ttatctgagt atgatgcagc tttcaaggaa
     1321 ttagttgaaa aatgatctat ctaagtgaaa aagatattgt aaaattgaat gttattcaaa
     1381 taaagaaata ttcaccgcga gaaacaatcg gagtcattga caaaaatgcg ctgagtatgg
     1441 ctgtaaatca acctaaacag catatttttg gaaaagatct gtatcctgat aaatttagca
     1501 aagcagctgt tctgctaata aatctgatca aaaagcatcc atttcataat gctaacaaac
     1561 gtacagcatt tttagccgcg tatgtttttc tcaagctaaa tggctattca ctaaaaatga
     1621 aaaatcagga agtagtagaa tttgtagttg aaattgctac gtatcagggt gattttgatg
     1681 acttaaaaga ggcaacaaca gatatactaa aaaagaattc aacaaagacc gattcaaaaa
     1741 agtagaagca aactagggat tcagttaaat ctgaagattt agtttgttct ttattttagt
     1801 ttacataaga tacattatgc gaagttcatt attttaaaag cttaaataat aaactttttt
     1861 ataataccaa tttatttatg taaaccaatg accttaatcg cttaagcaag catgttatgt
     1921 aatactctac tctctccaaa actaatgact atctattttc aaagaagcta gaatgattat
     1981 ctgatcaaat gataatttat atttaattcc tggtctactg ctc
//
LOCUS       DAZKLD010000236         2003 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.241, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000236 DAZKLD010000000
VERSION     DAZKLD010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2003
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.241"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..378
                     /locus_tag="ACG8KR_002895"
     CDS             <1..378
                     /locus_tag="ACG8KR_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HFQ0202186.1"
                     /translation="KNVSTEDFFNYCRLEESGVTVTSGTDLPLFITDIPESIIRAVFR
                     RFPEGEEVWEKETSFTTTELLKSFTSNSFKVNGLSEYGEIKVGKSASLAVFSEDLCTD
                     DWQKVMAAEVIATYLDGDLVYKK"
     gene            426..1826
                     /locus_tag="ACG8KR_002896"
     CDS             426..1826
                     /locus_tag="ACG8KR_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HFQ0202187.1"
                     /translation="METMKRKFTFPHSYTLIFILIILAAILTWIIPSGQFETVVENVA
                     GMEKTTVVPGTYHQIDKADYGQGFSSILMAPAEGIISAVEVVAFVLIVGGAFGIILKT
                     GAIDRGLFTLAKVLGDKGILVIPMSMILFSLGGSTFGMSEEVIPLFAVFISLMFSLGY
                     DSMTAILILFLSTQAGYVGSTINPFNVLIAQGVAGVYGNPLLWFRMLSWIVITGISIV
                     FTMLYASRVKKNPLSSIVYESDLKVKHKFQNFDTEKEFSTQDKLIIAGFIVGLGIMIW
                     GIISKGWYMVEISALFLGLGLFAGGIARFSQKEIAEAFVEGCSDFAYAAVIIGLARGV
                     LVILEGGMIIDTILNSLANLLGGLPKFLFTTILLISQVIVSFFVPSSSGAAALTMPVM
                     APLGDLMNVSRDVVVLSNQFGNGLMNIISPTSGVLLAGLAIAEINFSKWFKVGFKIFA
                     ILTLVSAVLLYIATLF"
BASE COUNT          555 a          339 c          432 g          677 t
ORIGIN      
        1 aaaaatgttt ccacagagga cttctttaat tattgtcgat tagaggaaag tggtgtaaca
       61 gtcactagcg gtacagattt accgctgttt attactgata ttcctgaatc gattataaga
      121 gcagtattcc gtcgttttcc tgaaggagaa gaagtctggg agaaagaaac aagcttcacg
      181 acaaccgagc tgctgaaaag ttttacaagc aacagcttca aagtaaatgg tttatcggag
      241 tacggagaaa taaaagtagg gaaaagcgcc agcttagctg ttttcagcga ggacctctgt
      301 actgatgact ggcaaaaagt gatggctgcg gaagttatcg ctacctatct tgatggtgac
      361 ttagtttaca agaaataatg cttttcgata taacgttata aaacgaatta aagagaggtt
      421 aaaaaatgga aacaatgaaa agaaaattca ccttcccaca tagttataca ttgattttta
      481 tcttaattat tttggctgca attttaactt ggataattcc tagtggtcag tttgagacag
      541 ttgttgaaaa cgtcgcaggt atggaaaaaa caacggttgt tccgggaacc tatcaccaaa
      601 tcgataaggc agattatgga caaggttttt cctctatact aatggcacca gctgaaggga
      661 ttatttcagc tgttgaggtt gttgcttttg ttctcatcgt tggtggtgcc ttcgggatta
      721 tcctaaaaac gggagccatc gatcgagggc tgtttacctt agcaaaagtt ctgggagaca
      781 aggggatctt agttattccg atgtcgatga ttctattcag cttaggagga tcaacctttg
      841 gcatgagcga ggaagtcatt cccttatttg cggtgtttat ttctctgatg ttttctcttg
      901 gctatgattc catgactgcg atcttgattc tatttttaag tacacaagcg ggatatgttg
      961 ggtcgacgat taatcccttc aacgttttaa tcgcacaggg agtagctggc gtttacggaa
     1021 atcccctgct ttggtttcgg atgctatcct ggattgtcat tactgggatt tcaattgttt
     1081 tcaccatgct ttatgctagc agagtcaaaa agaatccact gtcctctatt gtttatgaga
     1141 gtgatttaaa ggttaagcat aagtttcaaa attttgacac tgaaaaagag ttttcaacac
     1201 aagataaact aattatcgcc ggttttatcg taggtttagg aatcatgatt tggggtatca
     1261 tttccaaggg atggtatatg gttgaaattt ctgcactatt cttaggatta ggtctttttg
     1321 ctgggggcat cgctagattt tctcaaaagg aaattgctga agcttttgtc gaaggctgtt
     1381 cagatttcgc ctatgctgct gtaatcattg ggctagctag aggagtcttg gtaatcttgg
     1441 aaggtggaat gattattgac actattctaa acagcttggc taacctttta ggtggtcttc
     1501 caaaattcct gttcactacg attctactca tttctcaagt aattgtttcc ttttttgttc
     1561 catcctcctc aggagctgcg gcattaacga tgccggttat ggcaccattg ggtgatctaa
     1621 tgaatgtaag tcgtgatgtt gttgttctaa gtaaccagtt tggaaatggt ttaatgaata
     1681 ttatctcccc aactagtggt gtattactgg caggattagc catcgcagag attaatttct
     1741 ctaaatggtt taaggttggc ttcaaaatat ttgccatctt aacccttgtt tctgctgttt
     1801 tgttatacat tgctacactc ttttagtctt aatcatccat taataggagg caatagttat
     1861 gattaaaata aatcaattag caggaatgaa tatccactat ttattttatt ctctcaaaga
     1921 atttttcgct tatcaacaga aaattggtat ttcttctgtt gaactttggg gaggtgctcc
     1981 gcattttttc atagatgctt tta
//
LOCUS       DAZKLD010000237         1942 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.242, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000237 DAZKLD010000000
VERSION     DAZKLD010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1942)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1942)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1942
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.242"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            99..356
                     /locus_tag="ACG8KR_002897"
     CDS             99..356
                     /locus_tag="ACG8KR_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0202188.1"
                     /translation="MANLYIENKGDLNAIITLIVVMVKSIGNITIGMLFIILAAVLIF
                     MIVIWFLNERSKDNQHRRDMEIKSIESIDYLSIITLKKPRS"
     gene            complement(608..1180)
                     /locus_tag="ACG8KR_002898"
     CDS             complement(608..1180)
                     /locus_tag="ACG8KR_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFQ0202189.1"
                     /translation="MTKYGYARVSTASQSTKEQIQQLLENGVEKKNIFSEKFTGTKRD
                     RPKFNQLLAGLKPGDEIVVTKLDRFARNTREALEIIEPLLDDNVTIKVLNLGTIENTS
                     MGRMVTRTLLSVAEMERDMIVERTQEGKMFARKNNPNFREGRPKATITPKKRHAYELL
                     TSGKSYKEVEAITGFSRSTLFRIKKKIEES"
     gene            complement(1196..1801)
                     /gene="fic"
                     /locus_tag="ACG8KR_002899"
     CDS             complement(1196..1801)
                     /gene="fic"
                     /locus_tag="ACG8KR_002899"
                     /EC_number="2.7.7.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0070733 - AMPylase activity [Evidence
                     IEA];
                     GO_process: GO:0018117 - protein adenylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein adenylyltransferase Fic"
                     /protein_id="HFQ0202190.1"
                     /translation="MILENKLGLTNQVELAKVEEKLSKQKAKELYDCGKINEIEVGTF
                     KGLSEIHEFLFSDIYDFAGKIRSVNIAKGNFRFAPVMYLEHSLQHIDQMPQTSFEEII
                     KKYVEMNIAHPFREGNGRSTRIWLDLILKEELQKVVDWNLIDKADYLSAMERSPINDL
                     EIRYLISNALTDKIDDRELYMKGIDVSYFYEGYSEYTIDDL"
BASE COUNT          583 a          383 c          291 g          685 t
ORIGIN      
        1 actattagaa cacttgctat tacagaaaaa ggtatgctag ccacgcttac tatttattgg
       61 tatcaaacaa gtatcttaat ttgaaaggaa gatgatttat ggctaattta tatatagaga
      121 acaaaggaga cttgaatgcc attattactt taatcgtagt tatggtaaaa tcaataggta
      181 atatcacaat aggaatgctg tttattatat tagcagcagt gctgattttt atgatagtta
      241 tttggttctt aaatgaaaga tctaaagaca atcaacaccg tagggatatg gagataaaat
      301 caatagagtc aatagattat ttatcgataa taacactaaa aaagccacgt tcctaaacgt
      361 gacttacgct gaacgatatg tgttgtttgg tagacgaaca aatcgttcat tataaatata
      421 tgatggtatc ctaatatagt aagcgtggct agcatacctt taattacttt accttttaca
      481 gttaccatac acattttttg agcagaaatc aagtaaataa aaaaatatta tatttcacaa
      541 catgctaata gactcatatc tagaacgcat taagtattta cctgtctggt tcgctgagac
      601 ttctttttta actttcttca attttcttct ttattctaaa aagcgtactg cgactgaaac
      661 cggttatagc ttcaacttcc ttatacgatt tacctgaagt aagcagttca tatgcgtgcc
      721 tcttcttcgg tgtgattgtt gctttcggac gtccttctct gaaattagga ttattctttc
      781 ttgcaaacat ttttccttct tgggtacgtt ccacaatcat atcacgttcc atttctgcta
      841 cactcaacaa cgtccgtgtc accattcgcc ccatggacgt attttcaatc gttcctagat
      901 tcaaaacctt gatcgttacg ttatcatcga gtaaaggttc aatgatctct aatgcttctc
      961 tcgtgttacg agcaaatcga tcaagcttcg taacaactat ttcatctcca ggcttcagac
     1021 cagccaacag ctgattgaat tttggccgat cccgttttgt acccgtaaat ttttctgaaa
     1081 aaatattctt cttctctacg ccgttctcaa gcaactgttg aatttgttct tttgtacttt
     1141 gagaagcggt tgagacacgt gcatatccat atttcgtcat ttggtccacc tacttttaca
     1201 aatcatctat cgtatactca gaataccctt catagaaata gctgacatcg attcctttca
     1261 tatataactc gcgatcgtca attttatcag tcagagcatt ggaaattaga tagcgtattt
     1321 ccaaatcgtt tatgggactt cgctccattg cagaaagata gtctgcttta tcgattaagt
     1381 tccaatctac aactttttgc aattcttcct ttaaaatgag atccagccaa attctagtac
     1441 ttcggccatt tccttctcga aatgggtgag cgatattcat ctccacatat ttcttgataa
     1501 tctcttcaaa agaagtttgt ggcatctgat caatatgctg taacgaatgt tctaaataca
     1561 taactggtgc aaatcgaaaa tttccttttg caatattgac cgatcggatt tttccggcaa
     1621 aatcgtaaat atccgaaaat agaaattcat gaatttcaga taaaccttta aatgtaccaa
     1681 cttcgatttc atttattttt ccacaatcgt acaattcttt cgctttttgt ttactgagtt
     1741 tttcttcaac cttagccaac tcaacttgat tggttaatcc caatttattc tctaaaatca
     1801 tgcgaaatct ccttaacttt atgacataag tttatgaaac actctcaacc ccattatatc
     1861 gcgattttgt ttatgtgtca aaaactatta agtttctgac ccccgataga tttttcaagt
     1921 gtctctattt tgcacagtct ag
//
LOCUS       DAZKLD010000238         1892 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.243, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000238 DAZKLD010000000
VERSION     DAZKLD010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1892)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1892)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1892
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.243"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(336..791)
                     /gene="tnpA"
                     /locus_tag="ACG8KR_002900"
     CDS             complement(336..791)
                     /gene="tnpA"
                     /locus_tag="ACG8KR_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HFQ0202191.1"
                     /translation="MSNDDKSLAYTRWNCKYHIIFTPKYQRKVIYGQLRKDLGKILRK
                     LCEMKDVEIIEAHAMLDHIHMLVRVPPKICISSFMGYLKGRSAVLIHEQHANLKYKYG
                     NKSFWSKRYYVSTVGLNQKTNEKYIREQEADDRIRYIISKREYVEPFKK"
     gene            1034..1892
                     /locus_tag="ACG8KR_002901"
                     /pseudo
     CDS             1034..1892
                     /locus_tag="ACG8KR_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEfa10 family transposase"
BASE COUNT          600 a          368 c          355 g          569 t
ORIGIN      
        1 cttacgtaac tcaaaaatac ttccaaaggt gttcgataat ccagtgattt tctaggaaga
       61 ttatttctgc gccagcattt tctaatgttt gaatttgtgt tttgtaatct tggttgcttg
      121 tgctaattct tgaatagccg taaattgcca aaatttcatc accctttctg gacataagtt
      181 tctgacacca gttaaaatca agtctatcag taacccatac acccgtgagg acgggtgtat
      241 ttttaatccc cctagaaggg gtcgacactc gctgcccctt aaaaggggat ttttaaaaga
      301 ccgccaactg ctcatcttca ctcacttact tctctttatt ttttaaatgg ttctacgtac
      361 tctcttttac ttataatata tcgaatgcga tcatcagctt cttgttcacg gatgtatttt
      421 tcatttgtct tttgattcaa tccgactgta ctcacatagt atctcttcga ccaaaaggat
      481 ttgtttccgt atttatattt gagatttgcg tgctgctcgt ggattagtac cgcactacga
      541 cctttcagat accccataaa actggatata catatttttg gtggtactcg aacaagcatg
      601 tgaatatgat ctagcattgc atgtgcttct atgatttcca catctttcat ttcgcacaac
      661 ttcctaagta tttttccaag atcttttcgt aattgcccat agattacttt tcgttgatat
      721 tttggtgtaa aaataatatg atacttacaa ttccaacgag tatatgctaa acttttatcg
      781 tcatttgaca tgaaaaaact cctttcgatc tgatgagcgg ttggtagcca ctcactagta
      841 tatcgaaagg agtttttatc gctaaagata cttcttccac gcgtagaaac gcgcggtttt
      901 cttgtttcgc acgctttacg cacaaaactg actgaagtca ttaagaaaat aaaagggtct
      961 aaaactttta agttttgtac cgacccccaa aagttagacc aaaaaatcta acttttggag
     1021 gtcggtattt tttatggcta agtatacttt tgaacttaaa ttaaagatcg ttcatgatta
     1081 cttggatggt aaaggcggaa gtgattatct ggcaaagaaa tattccatta aggctccatc
     1141 acaagtgaag cgctggatca atgcctatca agaatttggt gaagagggtc tagtaagaaa
     1201 gcgacaaaag aaaaaatatt ctgttcaatt taagctggat gcgatagagt tatatctaac
     1261 aagtgagctg tcctatcgtg aagttgccaa cacacttaac atgaacaacc ctaatttgat
     1321 cgctagttgg atgcgtcaat tccgtgaggg tgggatcgat ggactctcaa aaacgaaagg
     1381 atgtccacct atcttgtcta agaaaaatga acccaaaaat aagaaaaaat ctacaactaa
     1441 agcaacgtct aaagaacgtg aacgcatcga ggagttggag aaacgagtcc gttcccttca
     1501 aattgaaaat gcctttttaa aagaattgag gaagctgcga aaacaggaag cccaacaacg
     1561 acgaatgaag caatcgcacg aatcatcgca agcctccgag gaccgtttaa attagttgag
     1621 cttctagaaa ctttaaagtt ccctaaggca atctttatgt actggcaaaa acgattggac
     1681 cgtaaaaatc cagatcaaga gattgaagcg gaaatgctta aagttcggga gaagcacaag
     1741 gattatggct gtttacggat gaccaatgaa ctaagaaacc gcggtttctc tatcaataaa
     1801 aagaaagttc agcgcctgat caagaagcta ggaataaaag taacgacata cacacgtaaa
     1861 tcacggcgct acaattctta tcgaggacaa gt
//
LOCUS       DAZKLD010000239         1862 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.244, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000239 DAZKLD010000000
VERSION     DAZKLD010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1862)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1862)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1862
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.244"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1765)
                     /gene="uidA"
                     /locus_tag="ACG8KR_002902"
     CDS             complement(<1..1765)
                     /gene="uidA"
                     /locus_tag="ACG8KR_002902"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HFQ0202192.1"
                     /translation="MLYPIMTETRQVIDLSGLWRFKLDTNDDLQEELALEPINEKESY
                     PISVPSSYNDLFETENIRNHVGWVWYEKEVTLPKRLLEERLVLRFGSATHEAKVFLNG
                     KFITQHKGGFTPFESEINRFVKDGNNRITVAVNNIIDDSTLPVGIMKETVQENGDIKK
                     ENLVNFDFFNYAGIHRPVKLYTTPKDYISDITINTRITDLDASVEFNIDTEWTGKILI
                     AIYDEEAQLVAEAEGKKGMIQLKNPIFWEPLDAYLYSMKISLIDDNKVIDTYTEEFGI
                     RSVQVTNGKFLINNKPFYFKGFGKHEDSYVNGRGLNEAVNIKDFNLMKWIGANSFRTS
                     HYPYSEEIMRLADREGIVVIDEVPAVGLHLNFMATLLDDNVEKHNTWKEIRTENSHKT
                     VIKELIERDKNHPSVVMWSIANEPDSDSEGAKEYFEPLVELTKELDPQKRPVTIVTYL
                     KSTPDVCKVGDIVDVLCLNRYYGWYVSGGDLAHAKYLLKKELDGWMERCPDTPIMFTE
                     YGADTVAGFHDTVPTMFTEEYQVAYYEANHEVIDTCENFVGEQVWNFADFATSQGILR
                     VQGNKKGIFTRDRKPKMIAYYL"
BASE COUNT          576 a          392 c          226 g          668 t
ORIGIN      
        1 gtagatagta agcaatcatt ttaggttttc tatctctagt aaagattcct ttcttatttc
       61 cttgcactct taaaatccct tgacttgttg caaaatctgc aaaattccac acttgttcac
      121 caacaaaatt ttcacatgta tctattactt cgtgatttgc ttcgtaataa gccacttgat
      181 actcttctgt aaacattgtt ggtactgtat catgaaatcc agctactgta tctgctccat
      241 attctgtaaa cattattggc gtatcaggac atctttccat ccatccatct agctcttttt
      301 tcaacagata ttttgcatgt gccagatccc ctcccgaaac ataccaacca taataacgat
      361 ttaaacataa aacatcgaca atatctccta ccttacaaac atctggcgtt gattttagat
      421 aagtaacgat tgttacaggt cttttctgcg gatcaagttc tttagttaat tcaactaaag
      481 gttcaaaata ttctttagca ccttcggaat ccgaatctgg ttcatttgca atagaccaca
      541 ttacaacact tgggtggttc ttgtcgcgtt ctatcaattc cttgatcacc gttttatgag
      601 aattttctgt tcgtatctct ttccacgtat tatgtttttc cacattatca tctaataaag
      661 tggccatgaa atttaaatgt aaccctactg caggtacttc atcaataact acaatacctt
      721 ctcgatctgc taatcgcata atttcttctg agtaaggata atgtgatgtt ctaaaagaat
      781 tagcacctat ccatttcatt aaattaaaat ctttaatatt tacagcctca ttcaaaccac
      841 gtccattaac atacgaatcc tcatgcttac caaatccttt aaaataaaaa ggtttattat
      901 taatcaaaaa tttgccattg gtaacctgaa cactgcgaat accaaattct tctgtatagg
      961 tatcgatcac tttattatca tcaattaaac taattttcat actatataaa taagcgtcta
     1021 gaggttccca aaaaattgga ttcttaagct gaatcattcc tttctttcct tcagcttcag
     1081 ctacaagttg agcctcttcg tcatatatcg ctattaagat ttttccggtc cattctgtat
     1141 cgatattgaa ttctacactt gcatccaaat cagttattct tgtattaata gtaatatcac
     1201 ttatatagtc tttcggagtt gtatataact taacaggtct atgaatacct gcataattga
     1261 agaaatcaaa attaactaag ttttcctttt ttatatcccc attttcctgt actgtttcct
     1321 tcataatccc cacaggtaaa gtactatcgt caataatatt gtttacagca actgtaattc
     1381 gattattccc gtcttttaca aaccgattaa tttcagactc aaatggtgta aacccacctt
     1441 tgtgttgagt aataaacttt ccattcaaaa aaacttttgc ttcatgtgtt gcagatccaa
     1501 aacgaagaac taatctttct tctaataatc ttttaggaag agtcacttct ttttcatacc
     1561 acacccatcc aacatgattt ctaatgttct ctgtttcaaa taaatcatta taagaagagg
     1621 gaactgaaat cggataactc tctttttcat ttattggttc taaagcaagt tcttcctgta
     1681 agtcatcatt tgtatctaat ttaaatctcc agagaccgct taagtcaatt acttgcctcg
     1741 tctctgtcat aatgggatac aacatataaa ccactcctat ctaaatatta aataaatgta
     1801 tcctttaaac cacaataagc atctattgga cttactccct taaattcaga aagacccatt
     1861 ag
//
LOCUS       DAZKLD010000240         1841 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.245, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000240 DAZKLD010000000
VERSION     DAZKLD010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1841)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1841)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1841
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.245"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            673..1005
                     /gene="traE"
                     /locus_tag="ACG8KR_002903"
     CDS             673..1005
                     /gene="traE"
                     /locus_tag="ACG8KR_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraE"
                     /protein_id="HFQ0202193.1"
                     /translation="MSYTGILSLEDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYAGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIS"
     gene            1624..1800
                     /locus_tag="ACG8KR_002904"
                     /pseudo
     CDS             1624..1800
                     /locus_tag="ACG8KR_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 family protein"
BASE COUNT          634 a          262 c          375 g          570 t
ORIGIN      
        1 agttaataat taatttccaa cacaaaaaag agaacaaacg tttgcatgtt gctgtttttt
       61 agttataata atacgcgaag agaggtaatt tcatttttta aaaaagctaa aaaccttgat
      121 attacttctt ttcaggcaat acgaatatta ttcgcttaca tttttttgta taaatttagt
      181 tgttttaaat ttaattgtct gctacgcttg tgatgaatta attttcgttc tgcacttttg
      241 gacgataaat ttggacaatt gaaaatacaa aaaaaagaaa ggccctgtga gactcggaag
      301 gctggtaacc ttcgtcgacg tccacaggtg gcattaagac aacctttaat cccttaatgc
      361 gtttaccttt cagtgttacg gtattacccg caacggattt agtataacag atattcaaaa
      421 gtgataccac tttttttggc tgtgttaagg ttaaaaatac gtgatattac gatgaatcta
      481 tgcctaagca taatgttata aaaatgtccg tgtggctctt tgccgtatga cgaacgctga
      541 aattagaggg aacgcatgtt tggatgcgtt ccctcttttt tgtttgtcat gaacgaaaat
      601 caattctcta tttttgaaaa ataacaagaa aaacttatta aaaatattta aagtaagtgg
      661 gtggattatt ttatgtcata tacaggaatt ttgagtctcg aagatatttg tcactacggt
      721 aaacggtgta ccgctacaga gaaaatcacg aagaaactat caactggcca aagtaaaacc
      781 gttgtgcagt gtaagaaata tataatccag aaagacaaag tctcagagga aatgatttat
      841 tacgctggta aacaaaagca aataatttta aaagatccta tccctttaaa agaactatat
      901 ccaacaatca aacatgttta tgaccaaaac ggtgtactga ttggtcgaag aaaaaatgga
      961 gtcttgcgtt gtaccgcaaa agggatgggg cgtttaatca gctgaattga tcattctcat
     1021 aacttgtatt ttatcgtttt tcgcttgctt gaaaatattg tcgagacttc taaaagaact
     1081 tagaattact agaaaaataa ggagtagact tgatctatca acaattcctt tcggggggtg
     1141 acggggtagg tttttacgat tggtaacgta aaaaattggt caatcgagta aatgcccttg
     1201 tttggatagt ggtgaaatga atttgtgtaa ataatatgca cagagtaaac aagagggaat
     1261 ttagaagtta gacaagttag aatagcgcct agattaatca tgagcagcta ctaagggatc
     1321 taggctttga cgcaaactca tggaataagg agtaggtctt ctcgtaatgg ggaggacgca
     1381 caagaaattg tgtcgtgtgc tgcagtacat gtagatactt gcgaggacgt tggtcattgg
     1441 atcaatcgag catttaccga tttctactag ggtttctcgt ttcggacgcc ataaacgagt
     1501 cgatattttt tatgtatttt taagtaaacg aaaaacgata aaatatgaac gagtcctgaa
     1561 taaagtgaaa aaattgatat aaaacataga gaaaatgaaa tttacgtaat ggaggaatga
     1621 caaatgaaaa attatgatcc gaatatacga ttgggcacac atactattaa ggtttctttc
     1681 caacgttggg attacaaagg atttgtgacg tttagaagag gcggaaattg taaaggatta
     1741 gatgttttag cattagacga ggatgattta tactatcaaa aattgactga taatccaatt
     1801 tgattcggct tgctacctga agatgatgaa ggtaatgaat g
//
LOCUS       DAZKLD010000241         1775 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.246, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000241 DAZKLD010000000
VERSION     DAZKLD010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1775)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1775)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1775
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.246"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            254..1180
                     /locus_tag="ACG8KR_002905"
     CDS             254..1180
                     /locus_tag="ACG8KR_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003953 - NAD+ nucleosidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HFQ0202194.1"
                     /translation="MTGRNPKVFISYSWENKENKEWARELADKLLENGIDAYIDQYDL
                     SLGDRLPQFMEQQISDSDYVLIICTPEYKKKANNRTSGVGYEGHIISAELLSGKNERK
                     FIPIIKEGRASEVLPTFLAGKLGIDLSDSQHYDENLDDLITTIFGVKRKPALGDIPRH
                     IKGSPLLPIEHNENKDIEILGIITDKVTVPKMDGSRGSALYKIPFRLNRVPSQLWKEL
                     FVRNWNSPPRYTTMHRPGIASVVDDEILLNGTTIEEVREYHRETLKLCIEKSNTQEKK
                     ILLEEEQKKERENERVRSHKESVQKLADELEF"
     gene            complement(1177..1773)
                     /locus_tag="ACG8KR_002906"
     CDS             complement(1177..1773)
                     /locus_tag="ACG8KR_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFQ0202195.1"
                     /translation="MQQNPSIDQNEQRQIEEMRKFGAEKIFIEKQSGATITHRPIFQE
                     VLDFVRDQDIFIVEAIDRLGRNYDEIIDSVNYLKKKNVRLIITNLPIMAEAIGNPLLD
                     KFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSANTKDPQRRLV
                     YRSIVQDQENGVAISKIATDYNVTRQTVYRIKKELTIN"
BASE COUNT          609 a          262 c          335 g          569 t
ORIGIN      
        1 aattaatgtc taacgtactg tacacgataa agtaggaaga cttattttca acttcggatt
       61 cattttttta attcggtgaa aatcactaaa ttaagagttt atccaagttc attttagctg
      121 agaatacgct tgaaaataat tttgatgaat tttcctgaat taaaatttat taacttgttg
      181 agtgatataa ttgtaatgta ttaccaaata gttagcactt agattagtga tggaaaggaa
      241 tagtgagaac aagatgactg gtagaaatcc caaagtattt atatcatatt catgggagaa
      301 taaggaaaat aaagaatggg ctagagaact agcagacaaa ttacttgaaa acggtattga
      361 tgcatatata gatcagtatg atttaagttt aggtgataga ttacctcagt ttatggagca
      421 gcaaatatcc gattcagatt atgttctaat tatatgtaca ccagagtata aaaaaaaggc
      481 aaataatcgt acaagtggtg ttgggtatga aggtcatatt atctcagcag aattgctttc
      541 tggaaaaaat gaacgtaaat ttatcccaat aattaaagaa ggacgtgcaa gcgaagtact
      601 tcctactttt cttgctggaa agcttggaat tgatttatca gatagccagc attatgatga
      661 aaacttagat gatttaatta caactatatt tggtgttaag agaaaaccag cattaggtga
      721 tatacctagg catattaaag gcagtccttt actacctata gaacataatg aaaataaaga
      781 tattgaaatc ttaggaatta tcactgataa agtaactgta cctaaaatgg atggctcaag
      841 gggttctgca ttatataaaa ttccatttag attaaataga gtaccaagtc agctttggaa
      901 agagttgttt gttagaaatt ggaattcacc accacgatat acaacaatgc atagaccagg
      961 aattgcttct gttgtagacg atgaaatttt acttaatgga acaactattg aggaagtccg
     1021 ggaatatcat cgggaaactt taaaactttg cattgaaaaa agtaatactc aagaaaaaaa
     1081 aatattgcta gaggaggaac aaaaaaaaga gagagagaat gagcgagtca gatcacataa
     1141 agaaagtgtt cagaaattag cagatgaatt agagttctag ttgatagtca gttctttctt
     1201 aattcgatac acagtttgtc tagttacatt atagtctgtt gctatttttg aaatagcaac
     1261 accattctcc tggtcctgaa cgatgcttct ataaacaagg cgacgttgag gatcttttgt
     1321 gttcgcactg tataacttag gacgtccttt atagacgccg tttgctttag caatctttat
     1381 cccttgtgcc tggcgtcgtt ttgattcagt acgctcttgt tcagcaatca tggccaagat
     1441 ttgaatgatt aggtctttga taaatttatc caggagagga tttccgattg cttcagccat
     1501 gatcggtaaa ttagtgataa taagtcgcac atttttcttc tttagataat ttactgaatc
     1561 gataatctcg tcataattac gtccaagtcg atcgatagcc tctacaataa aaatatcttg
     1621 gtctctaaca aaatctagaa cttcctgaaa aatcggtcga tgagtaatag tcgctccaga
     1681 ttgtttctcg atgaaaattt tttcagcacc aaattttctc atttcctcga tttgacgttg
     1741 ttcattttga tcgatcgatg ggttctgttg caaag
//
LOCUS       DAZKLD010000242         1771 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.247, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000242 DAZKLD010000000
VERSION     DAZKLD010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1771)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1771)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1771
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.247"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            144..542
                     /locus_tag="ACG8KR_002907"
     CDS             144..542
                     /locus_tag="ACG8KR_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HFQ0202196.1"
                     /translation="MTIFETVVTDVGIEAEVFRKEKIMILFGDEAPETLTDYCYKIEL
                     NQVRKEITCDMKLCFDKQMYVITAVGDVVQTNLNELGHITIKFDGSHEPELPGTMYVE
                     DKEMPHLRKGTIIKIDQEDQNGGKLYGNHA"
     gene            526..1221
                     /locus_tag="ACG8KR_002908"
     CDS             526..1221
                     /locus_tag="ACG8KR_002908"
                     /EC_number="4.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HFQ0202197.1"
                     /translation="MEIMLDTANTKSIYSCQQFLNLTGVTTNPTILKKEGASNFFSHM
                     KEIKKIIGQIPIHIQTIGRTEEEMIEDARVIIEELGKDTFIKVPVNQAGLQAIKKLKK
                     EGYLITGTAIYTELQGYLAINNGADYIAPYYNRMQNQGIDAMKVIDSLRTEIERTQRP
                     TKILAASFKNCEQVTTAIRSGAHAITLGKELLDQVLEMPSIDKAVRGFQEDWVQTFQA
                     ETIGELKKVADYF"
BASE COUNT          676 a          255 c          342 g          498 t
ORIGIN      
        1 tccatacatc cattgtactc tgagaaatca cacaatatcg aaacaaattc cgttaggaat
       61 gtgttattca ataaatttta taatctagaa acattttgaa taaaaactat tagacaaatg
      121 atattttacg aggaggaaca gagatgacga tctttgaaac ggttgtaaca gatgttggta
      181 tagaagcaga agtattcagg aaagaaaaaa taatgatttt atttggagat gaagcaccgg
      241 aaacattaac agattattgt tataagattg aattgaatca ggtaagaaaa gaaataactt
      301 gtgatatgaa gctctgtttt gataaacaaa tgtatgtaat cacagcagta ggagatgttg
      361 tccaaactaa tttgaatgaa ttaggacata tcacgataaa atttgatgga tctcatgagc
      421 cggaacttcc aggaacaatg tatgtagaag ataaggaaat gccgcattta agaaagggga
      481 caatcattaa aatcgatcaa gaggatcaga atgggggaaa gctatatgga aatcatgctt
      541 gatacagcaa atacaaaaag tatttattct tgtcaacagt ttttgaattt aacaggcgta
      601 acaacaaatc ctacaattct gaaaaaagaa ggagcgtcaa acttcttctc gcatatgaaa
      661 gaaatcaaaa aaataatcgg acagattcct atccatattc agacaatagg aagaacggaa
      721 gaagaaatga tagaggatgc tcgagttatc atagaagaac tgggaaaaga tacgtttatc
      781 aaggtgccgg tgaatcaagc aggccttcaa gcgatcaaaa agttgaaaaa agaaggttat
      841 ttgattaccg gtacagctat ttacacagaa ttacaagggt atttagctat taataatgga
      901 gcagattata ttgcacctta ttataacagg atgcaaaatc aaggcattga tgctatgaaa
      961 gtgattgatt cgttgcggac tgagatcgaa cgtacgcaaa gaccaacaaa aatattagca
     1021 gctagtttca aaaattgtga acaagtcact acagccattc gatctggagc tcatgcgata
     1081 acgttaggta aagagctact agatcaagta ttagagatgc cctcgattga taaagcagtg
     1141 aggggcttcc aagaagattg ggtgcaaact ttccaagcag aaacgattgg tgaactaaaa
     1201 aaggtcgctg attacttctg aatcaatttc tccataaaat aaacgatagc cttctcgaaa
     1261 actcgaggag gctatctgac gattcaatat tcattaattt aaagtttggg tgactattac
     1321 taactattac ttatttctca tttaaacgat gtattcccta acctctaata catccttgta
     1381 actctgaagt tacgccagtt ttgaataaat ttcataaaaa caaatgaaaa gtgtcgcata
     1441 aaaatatctt tcgtgtttct ctagtataac agaataagaa gaacaagtcc agaagggata
     1501 tttttagtct tttttaaaat aacttttcag tcgagtttga gtttatgata gttgtttttg
     1561 aagcgatacg gcaagtgaaa aaatagggaa gcatgaaaga atctacttaa tggcgcattg
     1621 attcatgtga tagaaataaa aaactttttt aaattatatc ctcatgattg taacaaatcc
     1681 agtgatttgt tttggtacaa ataaaaaaag gccccgatga agttgtggca aaagtggttt
     1741 gcctcaaaaa taagaaggaa tttccaaaaa t
//
LOCUS       DAZKLD010000243         1737 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.248, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000243 DAZKLD010000000
VERSION     DAZKLD010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1737)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1737)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1737
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.248"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            485..>1737
                     /locus_tag="ACG8KR_002909"
     rRNA            485..>1737
                     /locus_tag="ACG8KR_002909"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          521 a          349 c          462 g          405 t
ORIGIN      
        1 tttacatgat atattcattt ctgttgtttt aagtagcaaa gcaataaaat agtattttaa
       61 aaaacatata aaatagttgt tgacatgtta gaaatgacat gataagataa ctgagttgtt
      121 aaaaaagaac cacttgtgaa gcttgattct atcgaaaaaa acttcaaaaa agttcttgac
      181 aaataacaaa tgataagtta tgataaatga gttgctgtaa gatttcgaaa aacgaaatca
      241 aaaaaacttt taaaaaagag ttgacaatta cttgtctctc tgatatgata taaaagttgc
      301 tacggcagcc aaaaacgaat cggggaacac tcctgatgaa gtagaccttt gaaaactgaa
      361 caaagtaaga caaaccaaat gtgtagggcg tcttgattca attcaagaca acaaacattt
      421 tttaacaagc aagcaatatg ctagcaaaca aattgagctt aacaatcgca agattgttcg
      481 aactttttat gagagtttga tcctggctca ggacgaacgc tggcggcgtg cctaatacat
      541 gcaagtcgaa cgcttctttt tccaccggag cttgctccac cggaaaaaga ggagtggcga
      601 acgggtgagt aacacgtggg taacctgccc atcagaaggg gataacactt ggaaacaggt
      661 gctaataccg tataacaatc gaaaccgcat ggttttgatt tgaaaggcgc tttcgggtgt
      721 cgctgatgga tggacccgcg gtgcattagc tagttggtga ggtaacggct caccaaggcc
      781 acgatgcata gccgacctga gagggtgatc ggccacattg ggactgagac acggcccaaa
      841 ctcctacggg aggcagcagt agggaatctt cggcaatgga cgaaagtctg accgagcaac
      901 gccgcgtgag tgaagaaggt tttcggatcg taaaactctg ttgttagaga agaacaagga
      961 tgagagtaac tgttcatccc ttgacggtat ctaaccagaa agccacggct aactacgtgc
     1021 cagcagccgc ggtaatacgt aggtggcaag cgttgtccgg atttattggg cgtaaagcga
     1081 gcgcaggcgg tttcttaagt ctgatgtgaa agcccccggc tcaaccgggg agggtcattg
     1141 gaaactggga gacttgagtg cagaagagga gagtggaatt ccatgtgtag cggtgaaatg
     1201 cgtagatata tggaggaaca ccagtggcga aggcggctct ctggtctgta actgacgctg
     1261 aggctcgaaa gcgtggggag caaacaggat tagataccct ggtagtccac gccgtaaacg
     1321 atgagtgcta agtgttggag ggtttccgcc cttcagtgct gcagctaacg cattaagcac
     1381 tccgcctggg gagtacgacc gcaaggttga aactcaaagg aattgacggg ggcccgcaca
     1441 agcggtggag catgtggttt aattcgaagc aacgcgaaga accttaccag gtcttgacat
     1501 cctttgacca ctctagagat agagcttccc cttcgggggc aaagtgacag gtggtgcatg
     1561 gttgtcgtca gctcgtgtcg tgagatgttg ggttaagtcc cgcaacgagc gcaaccctta
     1621 ttgttagttg ccatcattca gttgggcact ctagcaagac tgccggtgac aaaccggagg
     1681 aaggtgggga tgacgtcaaa tcatcatgcc ccttatgacc tgggctacac acgtgct
//
LOCUS       DAZKLD010000244         1699 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.249, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000244 DAZKLD010000000
VERSION     DAZKLD010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1699)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1699)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1699
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.249"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            81..692
                     /locus_tag="ACG8KR_002910"
     CDS             81..692
                     /locus_tag="ACG8KR_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFQ0202198.1"
                     /translation="MIDIISAITVNVLTALYQPFLFAVLTAYLFMYWYLFIVDNSRDP
                     KNLKDSVLIWLRYFKASSKFRRLFFLAFYFTLILFKTLLNRNMWMNPLSNVIGQWSLY
                     IIDNATGESRLTTECVENTMLFIPFCMLLLWWRDIKCDIIRTIYVGIKYVFLFSLSIE
                     FTQLFFRLGTFQLSDLFYNTLGGLIGALLYWLFYRLNKYIDLK"
     gene            874..1389
                     /locus_tag="ACG8KR_002911"
     CDS             874..1389
                     /locus_tag="ACG8KR_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase family
                     protein"
                     /protein_id="HFQ0202199.1"
                     /translation="MVVVMVKVFDIIKFSDFRGYTAVALDNETLFSIGIEFHIKQINQ
                     GFSHNKYTMRGLHYQSGEHAQGKIVMCLHGEIMNIALDIRQGSSTYGCVYVNTLSESN
                     GKCAYIPRGYAHGYLTMEDNTLVQWCVDNDFNSNAAKCISYESVDIEWPVSRQCFVIS
                     EKDKNVERLSF"
     gene            complement(1524..>1699)
                     /locus_tag="ACG8KR_002912"
     CDS             complement(1524..>1699)
                     /locus_tag="ACG8KR_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HFQ0202200.1"
                     /translation="TVETVFSSLEKLGCQNFNSRSVKGLESRFESILLAYSVLLSRAQ
                     RRFEGTLRYSLGY"
BASE COUNT          554 a          232 c          329 g          584 t
ORIGIN      
        1 aattatattt agaaaacgat tgctactttt agtaaggaga attatgagca aataaacatt
       61 tgctcataga gagatagaga atgatagata ttatttctgc tataacggta aatgtattaa
      121 cagcattata tcagccgttc ttatttgccg tattaacagc gtatctgttt atgtactggt
      181 atctatttat agttgataac tctcgagatc ctaaaaactt aaaagattct gtattaatct
      241 ggttgagata ttttaaagca tctagtaaat ttcgcagact cttttttctt gcgttttatt
      301 ttactttgat attatttaaa accttgctta atcgaaatat gtggatgaac ccgctttcaa
      361 acgttattgg acagtggtca ttatatatta tagataatgc tacaggagag agcagattga
      421 caacagaatg cgtggagaac acgatgctgt ttatcccttt ttgcatgtta ttgttatggt
      481 ggcgtgacat taaatgtgat attatacgca ccatctatgt tgggattaag tatgtgtttt
      541 tattctcatt aagtatagaa tttacacaac tgttttttag gcttggtaca tttcaactat
      601 cggatttgtt ttataacacc ctagggggct taattggtgc tttgctatat tggctattct
      661 accgattaaa taagtatata gacttaaaat agaactgctt tgaattttaa tatggttaat
      721 atttttctgc agacaggatt gtgtaatctc atcaagaata ctttacatgc catctatttt
      781 tgctaaaaca ttgttgttat atcttcaaga ggtagggaat tttattgctt taaatgaggt
      841 cgagcgaatt aaaaaattgg taaaatatat aacatggtgg tagttatggt aaaagtgttt
      901 gatataatta agttttcgga tttcagagga tatactgctg ttgcattgga taatgagact
      961 cttttctcga ttggtattga gtttcacatt aagcaaataa atcaaggatt tagtcataat
     1021 aaatatacta tgcgagggct acattatcag agtggagagc atgctcaagg aaagatagtg
     1081 atgtgcctac atggtgaaat tatgaatatc gcacttgata ttcgacaagg atcttccact
     1141 tatggatgtg tgtatgtcaa cacattgagt gaaagcaatg gtaaatgtgc gtatatccca
     1201 cgtggatatg ctcatggcta tcttactatg gaggacaata cgctagttca gtggtgcgta
     1261 gataatgatt tcaattctaa tgctgccaaa tgtataagtt atgaaagcgt agatattgaa
     1321 tggccggtca gtcggcagtg tttcgttata agtgagaaag acaaaaatgt ggaaagatta
     1381 agcttttgat taaaatgagc tgtggagaaa agaaggatac tggaaaataa gaagaacaat
     1441 aaccgagcaa atgctcggtt atttctatga ggaatacata aaactaaatt tagatataga
     1501 tatacccgtg gtgctagtta attttaatat cctaaagaat atctcaaagt tccttcaaaa
     1561 cgtcgttgtg ctcgacttaa tagaacactg taagccagta atatgctttc aaatctactc
     1621 tctagccctt taacagaacg tgagttgaaa ttttgacaac ctaatttttc taaggaagaa
     1681 aaaacagtct caactgttt
//
LOCUS       DAZKLD010000245         1667 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.250, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000245 DAZKLD010000000
VERSION     DAZKLD010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1667)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1667)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1667
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.250"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..238)
                     /locus_tag="ACG8KR_002913"
     CDS             complement(<1..238)
                     /locus_tag="ACG8KR_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HFQ0202201.1"
                     /translation="MFSDKEVLLKSLHVCLKLPIVVFNSEFSIIKEYRSDRTTYFFYD
                     YKKLLTDNIKKTKDFHFFTGDFNEMFLLYMFKTRY"
     gene            932..1318
                     /locus_tag="ACG8KR_002914"
     CDS             932..1318
                     /locus_tag="ACG8KR_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFQ0202202.1"
                     /translation="MSDNDERVNAQLIRFFEKVILNTANTYYKKQEEIQKHEQLDQLP
                     PYLFTKDTIKFKQPINVLNVTLLVEDIKLAKILPLLKEPEQIFLIEKFIFDKTDKEIG
                     KVLGITRQGATNLKHRLYKKIQKMLV"
     gene            1412..1603
                     /locus_tag="ACG8KR_002915"
     CDS             1412..1603
                     /locus_tag="ACG8KR_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0202203.1"
                     /translation="MNSLKEDFILAKAGNEEAVEAILKRFSSLIHKQSWRTGKYDQDC
                     YQECMLAIYLAISKFEIKE"
BASE COUNT          625 a          250 c          229 g          563 t
ORIGIN      
        1 aatatctggt tttaaacatg tatagaagaa acatctcatt aaaatcacca gtaaagaaat
       61 ggaaatcttt agttttttta atattatcag ttagtaactt tttataatca taaaaaaaat
      121 aagtagttct atctgagcga tactctttta taatactgaa ttcggaatta aaaaccacga
      181 taggtaactt taaacaaaca tgaagagact ttaataagac ttctttatct gaaaacactt
      241 ttgtttctct ctttcctttc tacatcatta aatagtattt tatacgttat ctaaaatact
      301 attcctctgt caaaataagc taacttactt cattataaaa tatccactta tcgctttcat
      361 gtatattcca ccgtaaaaag tacattttat caccgagaac tcatcatttt tcatcatgat
      421 gaatactatt tacatatatc tagttcaaag acaataatta ttatgatccg ttattatcta
      481 agcactatac agataatagg aatttttcgt ttctaaatat tccaatttgg gaatccaata
      541 aaaattttcg ttcaaatacg tgtctattcg aactcttttc tagttgttaa ttatgaaggg
      601 ggaagaaata aaaaatacaa tctatacacg aataagaata ttatccaaaa ggaggtttta
      661 gaaaagattt attatacgta aaaagctcaa gacattttct tagccataag gacatgtttg
      721 atatttaaga agatatatga attttctttt ctaagtataa agataactat ccattatcaa
      781 ctaaaaaaac atttttgaaa ataaacatca gcagtcctat caatgcatgt gagcttatag
      841 aagttattcg cagcgcagca taattgcatt agcttctttt cgtgtttttt gcctcttttc
      901 cctttaaatc ttaatggagg aggtgtgcaa tatgtcagat aacgacgaac gcgtaaatgc
      961 ccagttgatt cgattctttg aaaaggtcat tttgaacacc gctaatactt attataaaaa
     1021 acaagaagaa attcaaaaac atgaacaact tgatcaactc ccaccatatt tgtttactaa
     1081 agatactata aaatttaaac aaccgattaa tgttttaaat gtgactctcc ttgttgaaga
     1141 tataaaatta gcaaaaatcc ttcctttgtt gaaagaacct gaacaaattt ttcttatcga
     1201 aaaatttatc tttgataaaa ctgataaaga aattggaaag gttctgggga ttacaagaca
     1261 gggtgcgaca aatttaaaac atcgacttta caaaaaaatt caaaaaatgc tggtttaata
     1321 tctgcataat aactaatttt gtatgcttct ttgctcgttc tcctccttcc caaagaaata
     1381 gattataagt ccatgggaag aaggtagatt tatgaattct ttaaaagaag atttcatttt
     1441 agcaaaggct ggaaatgaag aagctgttga ggcaattctc aaaagattta gctctttgat
     1501 tcataagcaa tcctggagaa ctggaaaata tgatcaagat tgttatcaag aatgtatgct
     1561 tgcaatatat ctagctatct cgaaattcga aattaaagaa taactcaact aagggagtct
     1621 gtgacataag taaaaagata gtttcaaatt tcgaacaata aatttga
//
LOCUS       DAZKLD010000246         1618 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.251, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000246 DAZKLD010000000
VERSION     DAZKLD010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1618)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1618)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1618
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.251"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..649)
                     /locus_tag="ACG8KR_002916"
     CDS             complement(<1..649)
                     /locus_tag="ACG8KR_002916"
                     /inference="COORDINATES: protein motif:HMM:NF024252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0202204.1"
                     /translation="MFDKNINSEIGKRIQEIRVSLGYTLEEFGENLTPPASKSNVSRW
                     EKGKNIPNKARLLSIAKLGGVSTEYLLTGNSQKTKKYIKYLFNEIFFDNGTFHNKFKD
                     FGKDRDRLGLYDLFLAVSKTYSLDKSPSLKDNFVEMIIDDVYKKLLQTDKSDDQLILG
                     FIYICLDEKLSNRQKSLKSINYEVFNYLGVAFGLIDSEIKSTNAGNDEDLLNYLKE"
     gene            813..1019
                     /locus_tag="ACG8KR_002917"
     CDS             813..1019
                     /locus_tag="ACG8KR_002917"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFQ0202205.1"
                     /translation="MKPKDFRKFCGLSQSELAEKYGISVQAVRNKESGKTNYSPEEMI
                     ILKYLVKEKGIENITIDQIFFNEK"
     gene            1070..1351
                     /locus_tag="ACG8KR_002918"
     CDS             1070..1351
                     /locus_tag="ACG8KR_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0202206.1"
                     /translation="MKSEIQVILPSDLDQELRRYFYRVAKEAVDQVRIETSLSKEVFR
                     LGEACKYLSCSRSTLKKFIREKGLKVSIIGDCQFIRKEELARFIKEYEV"
BASE COUNT          598 a          209 c          281 g          530 t
ORIGIN      
        1 attcttttaa ataatttaat aaatcttcat cgttacctgc gtttgtagat ttgatttcgc
       61 tatcaattaa accaaatgca acacctaaat aattgaatac ctcataattg atacttttaa
      121 gacttttctg tctgtttgaa agtttttcat caagacaaat atatatgaaa cccaggatga
      181 gttggtcatc tgatttatca gtttgcaaga gttttttata tacatcatct ataatcattt
      241 ctacaaagtt atcttttaaa gaaggtgatt tatctagact atatgttttc gatactgcta
      301 aaaataagtc atatagacca agcctatctc tgtctttacc gaaatctttg aatttgttat
      361 ggaaggtacc gttgtcaaaa aaaatctcat tgaataaata cttgatatat ttcttagttt
      421 tttgagaatt acctgtcagt agatattcag tagaaacacc accaagttta gcaatactta
      481 gtagccgcgc tttgttgggt atgttctttc ctttttccca tctagaaaca ttgcttttgc
      541 ttgcgggagg tgtcagattt tcgccaaatt cttcaagagt ataccctaaa gaaacacgta
      601 tttcttgtat tcgttttcca atttcagaat taatgttttt atcaaacaaa atggtttcac
      661 caccttcatc tattctattc tacagtttac actacattct catataatga aatgaaaaag
      721 tagataaaag ttgaatttta cgttgcggta tgaataaata tatgttattc ttttagtgac
      781 gaaaagttga aaagaaggag gagtgaggag tcatgaagcc taaagatttt agaaagtttt
      841 gtggattatc acaatctgaa cttgctgaaa aatatggcat ttcagtacaa gcggtaagaa
      901 acaaagaaag cggtaaaaca aactattctc cagaagaaat gattattttg aagtatcttg
      961 tgaaagaaaa aggaattgaa aatatcacaa ttgatcaaat tttttttaat gaaaagtgac
     1021 gaaaagttga atttgaatac atgtagtcaa attagtgggg gcaaagaaaa tgaaatctga
     1081 gattcaagtt attcttccat cggatttaga tcaagaattg cgaagatatt tctatcgagt
     1141 tgcaaaagaa gcagtagatc aagttcgaat tgaaacatct ctaagcaaag aagtttttag
     1201 attgggggaa gcttgtaagt atttaagctg tagcagaagt acattaaaaa aatttatcag
     1261 agagaaaggt ttaaaagtaa gcataatcgg agattgtcaa tttattagaa aagaagaatt
     1321 agcaaggttt atcaaagaat atgaagtata aaaagttgcg tggcgtgctt ttgatagaaa
     1381 aaaggaagtg tttgtttgaa taatgatgaa gtatagaaaa tataaaaata ctttgaaaaa
     1441 aattaaaagg agtgttcccg acagtttggc gaccacagga aacactcgac aataaagttc
     1501 taagaaaact ttatttgact acattttaac atttattaga aagaagagga ataaatggaa
     1561 gaaatagata aattaattgc tcagtatgca tactcaaaag atgacgtata tgctgaaa
//
LOCUS       DAZKLD010000247         1592 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.252, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000247 DAZKLD010000000
VERSION     DAZKLD010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1592)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1592)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1592
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.252"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..483)
                     /locus_tag="ACG8KR_002919"
     CDS             complement(<1..483)
                     /locus_tag="ACG8KR_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0202207.1"
                     /translation="MFEFNNVSFSYKDVKEQALVDLNFSIKKGSLTLITGSSGSGKST
                     LLKLMNGLIPRLYDGKLVGEILFENNSLQSLTQESISKNIGYVSQDPRGQFFTTNTTS
                     ELVFSMENFGFTKEHMDKRINELAELLDLSNILDKNIFSLSSGERQKISIGCSLSLNS
                     K"
     gene            complement(494..1162)
                     /locus_tag="ACG8KR_002920"
     CDS             complement(494..1162)
                     /locus_tag="ACG8KR_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HFQ0202208.1"
                     /translation="MSKINPLTLITLNIFFPVIIFLGKGNIFQVGCFIIATVLVLMFR
                     KIKTCIYFILIYSVLFLVMKIIDAIKIEFFTIFFATSLYIVFRMLPVVMISWLLVSVY
                     TSNELLSSLEKIHLPKKIMLSITVVIRFFPTYRSEIRMIKESLKMRNIRLSFFQPLQY
                     LEFLLVPVLIRATMIAEEMTANAITKGIESPVKRSSYYKTNMSLLDYIILFSVTIFFL
                     SILI"
     gene            complement(1292..>1592)
                     /locus_tag="ACG8KR_002921"
     CDS             complement(1292..>1592)
                     /locus_tag="ACG8KR_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFQ0202209.1"
                     /translation="KRIFDLILFQSAGTKYQDIVEKITSFVTKKTVCCLDKLKTIGMI
                     NQNLVFNEEEIHFYIYSFYATFYDILKHSYSKEKTMLLTRRLYQFSVPGWIELLS"
BASE COUNT          578 a          265 c          213 g          536 t
ORIGIN      
        1 tttagagttt agcgataaac tacatccaat agaaattttc tgccgttcac cacttgatag
       61 cgaaaaaatg tttttatcta atatatttga aagatctaat aattcagcta attcattgat
      121 tcttttatcc atatgctctt ttgtaaatcc aaagttttcc atcgaaaata ctaactcact
      181 cgttgtattt gttgtaaaga attgtcctct aggatcttga cttacgtagc caatattttt
      241 tgatatactt tcttgtgtta aggattgtag cgagttattt tcaaataaaa tttccccaac
      301 caattttccg tcataaagtc ttggaataag tccattcatt aactttaata gtgtggattt
      361 cccactccca gaagaaccag taatcaatgt aagactgcct ttttttattg aaaaattaag
      421 gtcaacaagc gcttgttcct taacatcctt atatgaaaaa gaaacattgt taaattcaaa
      481 caattctttc acctcaaatc aaaatactta agaaaaaaat agtaacggaa aacaggatga
      541 tatagtctaa taagctcatg tttgttttat agtaagaact tcttttcaca ggagattcta
      601 tcccctttgt tattgcatta gcggtcattt cttcagcaat catagttgca cgtataagaa
      661 cgggaactaa cagaaattcc aaatattgca gaggctgaaa aaaagacaag cgaatgtttc
      721 tcatttttaa agattcttta atcattctta tttcagaacg ataagtcgga aagaatctga
      781 tcactactgt aatacttaac attatttttt tcggtaaatg tattttttct aaagaggata
      841 ataattcatt gctagtatag acactaacca acaaccatga gatcattacg accggcaaca
      901 ttcgaaatac aatatataag gatgtcgcaa aaaatatagt aaaaaactct atcttgatag
      961 catcaataat tttcattact aaaaacagaa ctgaataaat caagatgaaa taaatacatg
     1021 tctttatttt acgaaacatc aatactaaaa ctgtagcaat aatgaaacac cccacttgaa
     1081 atatatttcc ctttcctaaa aaaataatga ccggaaaaaa aatatttaaa gtaatcaagg
     1141 ttaacggatt gatttttgac actctttcac ctccaaaaaa taagatcgca ctgttatctt
     1201 aagctgataa aaatgcaccg ttattcagat aacagtgcga tcttatcaaa aagcataact
     1261 gagaacaatt ctcaatgcaa gcttttttta attaactcag taattcaatc cacccaggaa
     1321 cagaaaattg atacaatctt ctggttaata gcatagtctt ttcttttgaa tagctatgct
     1381 tcaatatatc atagaatgta gcgtagaatg agtaaatgta gaagtggatt tcttcttcat
     1441 taaagactaa attttgattg atcattccga tagtctttaa cttatctaga caacagactg
     1501 tctttttcgt cacaaatgat gtaatttttt caacaatatc ttgatatttg gttccagctg
     1561 attggaataa aatcaaatcg aatatacgtt ta
//
LOCUS       DAZKLD010000248         1570 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.253, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000248 DAZKLD010000000
VERSION     DAZKLD010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1570)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1570)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1570
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.253"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(145..459)
                     /locus_tag="ACG8KR_002922"
     CDS             complement(145..459)
                     /locus_tag="ACG8KR_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0202210.1"
                     /translation="MDFYSNFILIIAILLLLNIWFFDKSRNAGIGFRTKRSTSSEKKW
                     VYSQTIFYGGVISISLLSSTLYSFNVIDVSMSNFISIIGILISAIITQLLLVFEEKSK
                     NN"
     gene            745..984
                     /locus_tag="ACG8KR_002923"
     CDS             745..984
                     /locus_tag="ACG8KR_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0202211.1"
                     /translation="MKRILFISLSNLLLLTACGTSASKNIPSDTQISNSIDSTISTNE
                     KNSPVETSDIKVSTSAKNRVKKLSARLLYFKQTLN"
     gene            complement(1346..>1570)
                     /locus_tag="ACG8KR_002924"
     CDS             complement(1346..>1570)
                     /locus_tag="ACG8KR_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0202212.1"
                     /translation="ICQPIGLKQKVIGEVISKLENCVVICVDEYHDVDRNEILEKCGK
                     VVAKYTHVYELETTEYLYNYSNKYEHTLVS"
BASE COUNT          545 a          277 c          216 g          532 t
ORIGIN      
        1 tataaactct agagaaatta attcatccta acataaaata attgttcact caaaagtttt
       61 tctagaacaa ttaaaacagc aggcttcaca ttcgctatac atttgtaaga agtaataatt
      121 cctgtactaa aaggcataat tgctttaatt attttttgac ttctcttcaa acacaagaag
      181 tagttgtgta ataattgcag aaataaggat tccaataata gatataaaat ttgacatgga
      241 aacgtctata acattaaaag aataaagtgt tgaagaaagt aaactgattg aaataacccc
      301 tccgtaaaaa atagtctgtg aatataccca ctttttttca gaagacgtac ttcgtttggt
      361 tctaaaacct atccctgcat ttctactctt atcaaaaaac caaatattta gaagtaataa
      421 tattgctatt attaagataa aattactgta aaaatccaaa agctatcctc ctagcaacta
      481 tttaaatacc caaaaaaatt acctctagat aatatcatac ctacgttttc acttataaat
      541 tttttcataa aaaaagcgtt tagaggcata atcgttaata cagatggtaa ccaatgtatc
      601 tggtttgaac tgggatatca ccctctccat atatgtatta ctaagactaa tagatactat
      661 aaaagacttg atagttttgc attgattttg cctaatactg agcataggtg tatgctaaac
      721 gatactataa ggagtgtgta cataatgaaa agaattctgt ttatcagttt atcaaattta
      781 ttgttactta ctgcttgtgg gacttctgca tcaaagaaca taccttcaga tacgcaaatt
      841 tcgaatagta tcgattcaac aatttctaca aatgagaaaa attctccggt agaaacttcc
      901 gatataaaag tttctacctc tgcaaaaaac agggtaaaga aactgtcagc acgtctcctt
      961 tatttcaaac aaactctgaa ttagcttctt catcttcttc agcaaataat gatgatagtt
     1021 tcgcgtttac tcctaactaa atgcaacctt tacaaatgct ttacgataac aagcctgagc
     1081 taaatgatcc tgatatcagt tttgcatttt ttgaaatgat tggatcggat gatcttttta
     1141 aaactactag tttgtctatc cgaaaatagg acagttctgg cagtataggt ttttatcgag
     1201 tttctccacc aggcctcgtt acagagacag attctctagg cgaacctttt gaataaatca
     1261 aaaatcatga acaatcactg ccaactaaat aaagtgcctt caaaagcttg gtcttctcgc
     1321 ttttgaaggc actttattta aatctttatg aaactaaagt atgttcatat ttgttgctat
     1381 agttatatag atattctgtt gtttccaact cataaacgtg agtatatttt gccactactt
     1441 ttccgcattt ttctaatatt tcattgcgat cgacgtcatg atattcgtct acgcagatca
     1501 caacacaatt ttctaattta ctgattactt ctccaatcac tttttgtttc aagccaatag
     1561 gttggcaaat
//
LOCUS       DAZKLD010000249         1553 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.254, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000249 DAZKLD010000000
VERSION     DAZKLD010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1553)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1553)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1553
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.254"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            74..496
                     /locus_tag="ACG8KR_002925"
     CDS             74..496
                     /locus_tag="ACG8KR_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0202213.1"
                     /translation="MKNKFILSLGILTIGISVTGTTIPTFADSTNEPTTVVDNNQELT
                     PLDRNGIAIGNPTNDLTVTENYLIVSPLLARAASKTIRVTGTMIFDKVGWPSRPPQSR
                     NWKEKRNGYWYYGKLYLQSYKSAGNNWIATYSGTLTRK"
     gene            506..709
                     /locus_tag="ACG8KR_002926"
     CDS             506..709
                     /locus_tag="ACG8KR_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0202214.1"
                     /translation="MPEFVNTPNFYQDSDILSKINLPKEESALQVQRTLQETQKIQKS
                     ISNLEDSEEKRKKRDQKTYQKEK"
     gene            920..1504
                     /locus_tag="ACG8KR_002927"
     CDS             920..1504
                     /locus_tag="ACG8KR_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFQ0202215.1"
                     /translation="MKIGYARVSTGLQNLDLQEDSLNKFGCEKIFTDHMSGAKSNRPG
                     LEMAIDFVRSGDTLVVWRLDRLGRNMEDLISIVNRLNERGVSFHSIQENITMDKSSST
                     GQLMFHLFAAFAEFERNLILERSAAGRAAARARGRFGGRPEKLSEQDLELLKTLVDSG
                     TPIKTIAERWNVSRTTIYRYLAKMEEKNGKTNSH"
BASE COUNT          550 a          222 c          287 g          494 t
ORIGIN      
        1 tatataactt gatttatctg ttaagattta ttaagaaaac gttttcttat ttttgatttc
       61 gaggtgatag acaatgaaaa ataagtttat tttatcttta ggtattttaa ctattggaat
      121 tagtgtaact ggaactacaa ttcctacatt tgctgatagt acaaatgaac caactactgt
      181 agtagataat aaccaagaat taactccttt agataggaac ggaatagcca ttggtaaccc
      241 aactaacgac ttaactgtta ctgaaaatta tttaatagtt tctccattac ttgctcgagc
      301 agcttctaaa acgatacgag tgacaggaac gatgattttt gataaagttg gttggccttc
      361 tagacctcct caatcacgga attggaaaga aaaaagaaat ggttattggt actatggaaa
      421 attgtattta caaagttata aatcagctgg aaacaattgg attgcgactt attcaggaac
      481 cttaactaga aagtagggat tttttatgcc agaatttgtg aacacaccaa atttttatca
      541 agatagcgat attctaagta aaattaattt accgaaagaa gaatcagctt tacaagttca
      601 gcgaacttta caggagacac aaaaaattca aaaatcaatt tctaatctag aagattctga
      661 agaaaaaaga aaaaaaagag accaaaaaac atatcaaaaa gaaaaataat gattttaaaa
      721 aaagagtgtc tagtcgacat tctttttttc taattgaaag tgttcataaa atcacccatt
      781 tctggacact ttcttttttt gctttatttt tcaaaaaaac aattcatttc atgttcataa
      841 acccataaca atatctatgt tcagttaaac tatatggata aagttatgtt acaataaaat
      901 taatgaagga gtgatttttt tgaaaatagg atacgcccgg gtatctactg gtttacaaaa
      961 tttggattta caagaagaca gcctaaataa atttggttgt gaaaaaattt ttactgatca
     1021 tatgagtggg gcaaaaagta atcgaccagg attagaaatg gccattgatt ttgttcgctc
     1081 tggcgataca cttgtcgttt ggcgcttaga tcgtttaggt agaaatatgg aagatttgat
     1141 ttcaatcgtc aatcgactga atgagcgtgg tgtgagtttt catagtattc aagaaaatat
     1201 tacgatggac aaatcaagtt ctacaggaca gctcatgttt catttgtttg cggcatttgc
     1261 agaatttgag cgtaatttga tcctggaacg atccgcagca ggacgagcag ctgctcgtgc
     1321 aagaggacgg tttggtggtc gtcctgaaaa attgtctgaa caagatttgg aattactgaa
     1381 gactttagtg gatagcggta cacctattaa gactattgcg gaacggtgga acgtctcaag
     1441 aaccaccatt tatcgttatt tggcaaaaat ggaggagaaa aatggaaaga caaattctca
     1501 ttagtttttg tcatttaatg ctgaaaattt ttgccattta tctagaaaag tga
//
LOCUS       DAZKLD010000250         1547 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.255, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000250 DAZKLD010000000
VERSION     DAZKLD010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1547)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1547)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1547
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.255"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(192..1007)
                     /gene="thiD"
                     /locus_tag="ACG8KR_002928"
     CDS             complement(192..1007)
                     /gene="thiD"
                     /locus_tag="ACG8KR_002928"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HFQ0202216.1"
                     /translation="MEDQYVQAMTIAGSDSDGSAGMQADMHTFFTRNVYGVSVMTACV
                     AGNSYGIGASVTLPTDFIDKEFELIAKDFQIRAAKTGMLADSKLIETVVKNYQKYDFG
                     PLVVDPVIVTKHGNLLLEESALQSLKEKLVPLAEVLTPNFYEAEKLTGRTLAAEEDFV
                     QAAKELQDMGAKNVMIKGHHAKNAQQETVNDYVLFESGKSFWLNAPFYPTDRINGTGD
                     SLSACIAAELAKGKTVEQAIRLAKDYVNRAIREEINVGHKYGPINHWAAGELE"
     gene            1301..>1547
                     /locus_tag="ACG8KR_002929"
     CDS             1301..>1547
                     /locus_tag="ACG8KR_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0202217.1"
                     /translation="MKLEDKKVILKKIKRLPDQIDGYTICSFKKDRSIKAYTQNTEKG
                     IIYFLHEEGYDTQTFMFTEKKEFHKQMKKLIEKEFPRS"
BASE COUNT          470 a          310 c          246 g          521 t
ORIGIN      
        1 agaagctgtt ttttgaactt gttccttgtg actcggagct ggacggcagg ctcacgacct
       61 ctttcttaaa cggtgttcaa aagcttgcat ctgcggtaat aagtccaacc aagttaaaaa
      121 cataagaagc tgtttttact tggctgttct tattacttga tgcagggcaa ctttttcaca
      181 acttctttct tttactctaa ttcaccagct gcccaatgat tgatggggcc atatttgtgc
      241 cctacattta tttcttcacg aattgcacgg tttacatagt cttttgccaa tcggatcgct
      301 tgttcaactg tttttccttt cgctaactca gctgcaatac atgcagatag cgaatcgcca
      361 gttccgttga tccggtcggt aggataaaat ggtgcattta accaaaatga ttttcctgat
      421 tcgaacaata cgtagtcatt gactgtttct tgctgcgcat ttttagcatg atgtcctttg
      481 atcataacgt tttttgcacc catatcttgc agttcttttg ctgcttgcac aaaatcttct
      541 tcagctgcta atgtccgtcc agtcagcttt tctgcttcat aaaaattagg tgtcagtact
      601 tcggccaaag ggacaagctt ttctttcaag ctttgcaaag cagattcttc tagtaaaaga
      661 tttccatgtt tcgttacgat tacgggatcc acgactaatg gtccaaaatc atatttttgg
      721 taatttttta caactgtctc aatcaacttt gaatctgcta gcatacctgt tttagctgca
      781 cgtatttgaa agtccttcgc aatcagttca aattctttgt cgataaaatc tgttggcaat
      841 gtcacacttg ctccgattcc gtaagaatta cctgcaacac atgcggtcat gacagaaaca
      901 ccgtaaacat ttcttgtaaa gaatgtatgc atatctgctt gcatccctgc gcttccatcg
      961 ctatctgatc ctgcgatcgt cattgcttgt acatactgat cttccatcaa atcgattcct
     1021 ccatttttat aaaaagattt ttcataatac tgttactgtt tctatcatat atttttcaaa
     1081 gtatccctca gtaaatatac cactaccttt tcttggtaat caaatttatt tgaaaaaaca
     1141 tttgcaataa atcagaaaat agtggtcgtt tatttgtatt cgctattcta ataataaata
     1201 accactattt gatttatata tatttcagta tccttcttgt tttcatataa ccaaaggcgc
     1261 tatgatttcc atatgaaaaa aataaagtca ggtgaaaaaa atgaaacttg aggataaaaa
     1321 agtcattttg aagaaaatca aacgactacc agaccaaata gatggctata cgatctgttc
     1381 tttcaaaaaa gacaggagta taaaagctta tacgcaaaac acagaaaaag gaataatcta
     1441 tttcctacat gaagaaggtt acgataccca aacctttatg tttactgaaa aaaaagaatt
     1501 tcataaacag atgaaaaaac tgattgaaaa agaatttcca agaagtc
//
LOCUS       DAZKLD010000251         1544 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.256, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000251 DAZKLD010000000
VERSION     DAZKLD010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1544)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1544)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1544
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.256"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(50..1090)
                     /locus_tag="ACG8KR_002930"
     CDS             complement(50..1090)
                     /locus_tag="ACG8KR_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HFQ0202218.1"
                     /translation="MTDFNFFKADRVYNELFYQFPKVFIVSDEYKKMKDSTKIAYMLL
                     KARLEIAISKRQIDEEGNVYFTYTTNELCSVLNCQKQKAIAIKKELESFGLLLQKQMG
                     FNKQLGKNNPNRLYLAELKVSENDIYLLEKFDRENRENVDKSEGMKIIPTLDEKSDAE
                     SLGAQEGMKIIPCQNVDKSEGMKIIPELNNNILDTNRHNIDTEKDRLQDQLLLDNFET
                     IMTNDSIATFIPERVLNLIKTFSSSYSEAQKTVQTIHNAKKKAETESGISIIFEELDS
                     YYVNAEQELYTTLLKAYQKLKTEKVENIQNLIFVYVKNWFIEKPIAAKVSSEKRLNSE
                     SFPKTITKDWLE"
BASE COUNT          499 a          241 c          203 g          601 t
ORIGIN      
        1 taattttaat gctactccat gaagcaacaa tcaatttgtc agtgaaattt cactctaacc
       61 agtctttcgt aatagttttt gggaagcttt cagaattcaa acgtttttca cttgatactt
      121 tagcagctat tggtttttcg ataaaccaat tttttacata gacaaaaatc aggttctgga
      181 tattttcaac tttttcggtt tttaattttt gataggcttt taacagtgtc gtgtataatt
      241 cttgttctgc attgacataa taactatcga gttcttcaaa aattattgaa ataccacttt
      301 ctgtttcggc ttttttcttt gcattatgaa tagtctggac agttttttga gcttcactgt
      361 aacttgaaga aaatgttttt attaaattta atactcgttc agggataaac gtagcaatgc
      421 tgtcgtttgt cataattgtc tcaaaattgt ctaacaacaa ttgatcttgt aggcggtctt
      481 tttcagtgtc tatattgtgt ctattagtgt ctaatatatt attattaagt tctggtatga
      541 ttttcatacc ctctgattta tcaacgtttt ggcacggtat gattttcata ccctcttgag
      601 ccccaaggga ttcagcgtct gatttttcgt cgagggtggg tatgattttc ataccctctg
      661 atttatcaac gttttcccta ttctctctat caaatttttc gagtaagtag atatcatttt
      721 ctgagacttt taattctgct agatatagtc tattaggatt atttttccct aactgtttgt
      781 taaatcccat ctgcttttgt aataataaac caaaggattc caactctttt ttgattgcta
      841 tcgctttttg tttttggcag tttaatacgc tacatagttc atttgtcgta taagtaaaat
      901 aaacattacc ttcttcatcg atttgccgtt tgctgattgc gatctctaat cttgctttta
      961 aaagcatata ggcaatctta gttgaatctt tcattttttt gtattcgtca gaaacaataa
     1021 agacttttgg aaattgataa aataattcgt tgtaaactct atccgcctta aaaaaattaa
     1081 aatcagtcat tgtagatcct ctactttcac gttgttttat ttatgtaaaa ttacctactc
     1141 ttttcgaaaa tagcaaagat aaataaaaaa tgcaaacgca ttaaaactgt tttttaatta
     1201 aatactacct aaaatcagaa ataaaatgaa ttatgtacta cctttaaagt cattaagaac
     1261 tactttaaat aaatattatt tataattaaa tactaccaga agaacgtatg aataaaattt
     1321 agttttaaaa ttaaagataa ttaagtacta ccagtgtcaa aacaaaaaat aaattaagaa
     1381 ctaccttatg catataaaac atactaccat tactagtaat atatactacc ttatttcagt
     1441 agtatatatt atattcttct tataagacaa ggctttaggc tagaaaacaa tagattatat
     1501 actactaaaa aatacaataa gtactaccaa tgtcagatat atat
//
LOCUS       DAZKLD010000252         1542 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.257, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000252 DAZKLD010000000
VERSION     DAZKLD010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1542)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1542)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1542
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.257"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            3..845
                     /locus_tag="ACG8KR_002931"
     CDS             3..845
                     /locus_tag="ACG8KR_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HFQ0202219.1"
                     /translation="MKKDEIIMGLLVPRANTYTIPLTKRQLIAKRYQRITRAINREFW
                     NSESDTAHSLYVGSYGRGTAISTSDIDIIVELPMAEFDRFKNYLSNGPSKLLQVIKNA
                     FQEILPNSDIRADGQVVKINFHDGIKFEIVPAFNEKDYLGESKGFIYPDSNMGGNWKA
                     TNPKKEQEAMKLKNTKSNNLLYATCKHFRHVRDTEFTSYHLSGIVIDSFVYEAMGNWK
                     FVENNSGGQNISSVSYETALLEYYNSHKVMGGLNLYSPGSNQFVNSDSSIICLEKVLK
                     KIAL"
     gene            865..1425
                     /locus_tag="ACG8KR_002932"
     CDS             865..1425
                     /locus_tag="ACG8KR_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLATT domain-containing protein"
                     /protein_id="HFQ0202220.1"
                     /translation="MGDEKKRLEVQLREAYGRVTYTYTTHLKQADNIISDNKILRCLQ
                     IGLSALTTGGVLSTIITDNSELTFVAGLCSTISLGLNLYFKNFDLAEEAKNHQIVADK
                     LWLIREEYVSLLTDLNRLSVEDIVKKRDQLMNKTHEAYKNAPKTNSKSYESAQKALKS
                     EEEQFFTEEELDKMLPEHLRKKSSKY"
BASE COUNT          588 a          202 c          277 g          475 t
ORIGIN      
        1 taattaaaaa ggatgagatt ataatggggt tactggttcc gagagctaat acttatacta
       61 tacctttaac gaaacgccaa ttaattgcta aaagatatca aaggattact agagccatca
      121 atagagagtt ttggaattct gaaagtgata ctgctcacag tttatacgta ggttcttatg
      181 ggagaggaac ggcaattagt actagtgata tagatataat agttgaatta ccgatggcag
      241 aatttgacag attcaaaaat tatttatcaa atggaccgtc taagttatta caagttataa
      301 aaaacgcatt tcaggaaata ctaccaaata gtgatattag agctgatgga caagttgtta
      361 aaataaattt ccatgatgga atcaaatttg aaatagttcc agcttttaat gaaaaagatt
      421 atttgggtga gtcaaaaggg tttatttatc cagattcaaa catgggaggg aactggaaag
      481 ctacaaatcc caaaaaagag caagaagcca tgaaattaaa gaatacaaaa agtaataatt
      541 tattatatgc tacgtgtaaa cattttcgac atgtacgaga tacagaattt acgagttatc
      601 atctttcagg aatagtaatt gacagtttcg tatatgaggc tatgggaaat tggaaattcg
      661 ttgaaaataa cagtgggggt caaaacatta gttctgtatc ttatgagaca gctttattag
      721 aatactataa ctcacataaa gttatgggcg gtttaaattt atactctcct ggaagcaatc
      781 aatttgttaa ttctgatagt agtatcatat gtttagaaaa agtattaaaa aaaatagctt
      841 tataaagttt tatggaggaa caacatggga gacgaaaaaa agcgcttgga agttcaatta
      901 cgtgaagcat atggacgagt aacgtatact tacaccactc atttaaagca agccgataat
      961 attatttctg acaataaaat tttaaggtgt cttcaaattg gtttgtcagc ccttactact
     1021 ggaggggtac ttagtaccat tattactgat aatagtgagt taacctttgt tgctggatta
     1081 tgttcaacaa tttctcttgg attaaactta tactttaaga attttgatct tgctgaagaa
     1141 gctaaaaatc atcaaattgt tgccgataaa ttatggttga tcagagaaga atacgtaagc
     1201 ttactaacag atttaaatcg tttaagtgtt gaagatattg tgaaaaaaag agatcaatta
     1261 atgaacaaaa ctcatgaagc atataaaaat gcccctaaaa caaatagtaa aagctatgaa
     1321 tcagctcaga aagcattaaa atctgaagaa gagcaatttt ttacagaaga ggaattggat
     1381 aagatgttac cagagcatct tagaaaaaag agttctaaat attgaactct ttttttatgc
     1441 ttaaaatttt cttgtaattt ttctattcaa tcaataaata atctatattt aagaagaaat
     1501 atgatgatag aaacatctcc taaagccacc gcaagccgtc ct
//
LOCUS       DAZKLD010000253         1508 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.258, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000253 DAZKLD010000000
VERSION     DAZKLD010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1508
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.258"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..672)
                     /locus_tag="ACG8KR_002933"
     CDS             complement(<1..672)
                     /locus_tag="ACG8KR_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFQ0202221.1"
                     /translation="MDNHTRKLLGLTDKHLFFDEEWLIEKEYKGVQAQFIKGRLDDSP
                     SHCEHCGSIRIIRNGSYTTRTQILKVKEKLTILELKRTRFLCYDCGQTFSAKTDLVDE
                     HHQLTKELKQAILIELYENQSRKLIAKKYFVSDGTVTRILREATKHYQPRMNFLPTVL
                     CMDEFKSMKSVSGSMSFICVDGTTHQLFTILEDRRLYKLTQYFLRFPRKARLKVKYLV
                     MDMNAS"
     gene            960..>1508
                     /locus_tag="ACG8KR_002934"
     CDS             960..>1508
                     /locus_tag="ACG8KR_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFQ0202222.1"
                     /translation="MKILTVEDDNMIREGISEYLSEFGYTVIQAKDGREALSKFNSDI
                     NLVILDIQIPFINGLEVLKEIRKKSNLPILILTAFSDEEYKIDAFTNLVDGYVEKPFS
                     LPVLKARIDSLIKKNYGHLEKFEYKDLSVNFNSYTAKINGEEIDVNAKELEVLKCLLD
                     NNGQVLTRMQIIDYVWKDSEETP"
BASE COUNT          515 a          227 c          289 g          477 t
ORIGIN      
        1 gctggcattc atgtccatca ctaggtactt gacctttaaa cgggcttttc taggaaagcg
       61 caggaagtat tgagttagtt tgtaaagtct gcgatcttca aggatcgtaa atagctgatg
      121 ggttgtccca tcaacacaaa taaaactcat ggaaccagaa acagacttca ttgatttgaa
      181 ctcatccata cacaacaccg tcggaaggaa gttcatacgt ggctggtaat gcttcgttgc
      241 ttcacgcaag atccgtgtca ccgtcccatc cgagacgaag tacttcttag cgattagttt
      301 acgtgattga ttttcgtaca gttctatgag aatcgcctgc ttcagttcct ttgttagttg
      361 atgatgttca tcaactaagt ccgttttcgc agaaaaggtc tgcccacaat catagcatag
      421 aaaacgggta cgttttaatt ctaaaatagt caacttttct ttgactttca atatctgagt
      481 acgtgtcgta taggaaccgt tgcggataat acgaatcgat ccacagtgtt cacaatggga
      541 aggtgagtca tccaatctcc ctttaataaa ctgcgcttga acgcctttat attctttttc
      601 aatcagccat tcttcatcaa aaaataaatg tttatctgtt aatccgagta attttctagt
      661 atgattatcc atagagcgac tcctttggta gggtgatgtt ttggtcgact aaattttacc
      721 aaaagtgagt ctctttttga gttgaaacga agaaaaagtg ttagtagaaa atcgccttcg
      781 attttccaac aacacaaaat attatagaac cctttattct aaaggatttc aaggtgtctg
      841 tttgcattta ttagaaaaaa gaaaaagtat tggcaacaga aggtgtcacc aacactagat
      901 attatagagc cataatatgc tgtataatat ctagtgtata ggcttaagtg gaggtatata
      961 tgaaaatatt aactgttgaa gatgataata tgataagaga gggaataagt gaatatcttt
     1021 cagaatttgg atatactgtt attcaagcta aagatggaag agaagccttg tcaaaattta
     1081 atagcgatat aaatttggtt atcttagaca ttcagatacc ttttataaat ggtttagaag
     1141 tgttgaaaga aattagaaag aaaagcaatt taccaattct aatcttgact gcatttagtg
     1201 atgaagaata taaaattgat gcatttacta atttagtaga cggatatgta gaaaagccat
     1261 tttcattgcc ggtcttaaaa gctagaatag attctttaat taagaagaat tatggacatt
     1321 tagagaagtt tgaatataag gatctatcgg ttaattttaa tagctataca gctaaaataa
     1381 atggtgaaga aatagatgta aatgcaaaag aactagaagt attaaaatgt ttattggata
     1441 ataatggaca agtgttaaca agaatgcaaa ttattgatta tgtatggaaa gatagcgaag
     1501 aaactcct
//
LOCUS       DAZKLD010000254         1484 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.259, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000254 DAZKLD010000000
VERSION     DAZKLD010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1484
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.259"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..822
                     /gene="panB"
                     /locus_tag="ACG8KR_002935"
     CDS             <1..822
                     /gene="panB"
                     /locus_tag="ACG8KR_002935"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HFQ0202223.1"
                     /translation="NTAVTFKESKLRNEKLTMLTAYDYSTAKIIDEAGINGILVGDSL
                     GMVCLGHEDTLSVTMEDMIHHTRAVTRGAKNTLVVADMPFMSYQTSVYDSVVNAGRLI
                     KEGRAQVVKLEGGIEVCDKIEAIVKASIPVMAHIGLTPQSVNAFGGFKVQGKDKEAAK
                     ELIRAAKAVEKAGAFAVVLECVPTKLAELISKEISIPTIGIGAGAGCDGQILVYQDML
                     GMYSDFTPKFVKKYANLSEEMNKAFTKYIEEVKDGVFPGPEHGFKISDDVLEKLY"
     gene            837..>1484
                     /gene="panC"
                     /locus_tag="ACG8KR_002936"
     CDS             837..>1484
                     /gene="panC"
                     /locus_tag="ACG8KR_002936"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HFQ0202224.1"
                     /translation="MVIFKTVNDVRSQVKEWKKQGLKVGLVPTMGYLHEGHESLIRKA
                     SKENDKVVVSIFVNPTQFGKNEDLGSYPRDLERDIEVCTRGRATAIFNPEVEEMYCDN
                     ASTFVNITGLTEGLCGASRPIHFRGVCTVVSKLFNIIPADRAYFGEKDAQQLAVIKRM
                     VRDLNIDIDVVGCPIIREEDGLAKSSRNTYLSLEERSSATILNKSLTLAKEALNNG"
BASE COUNT          566 a          161 c          326 g          431 t
ORIGIN      
        1 aatacagcag ttacatttaa agaatctaaa ttaagaaatg aaaaattaac aatgcttaca
       61 gcttatgact attcaacagc aaaaataatt gatgaagctg ggataaatgg aatattagta
      121 ggagattctc ttggtatggt atgtctagga catgaagata cactttcagt aacaatggaa
      181 gacatgattc atcatactag agcagttaca agaggagcta agaacacttt agttgttgca
      241 gatatgccat ttatgtctta tcaaacttca gtatatgatt cagtagttaa tgcaggaaga
      301 ttaataaaag aaggacgagc tcaggttgtt aaattagaag gagggataga agtatgcgat
      361 aaaatagagg ctatagtaaa ggcttctata cctgttatgg cgcatatagg tttaacacct
      421 caatctgtta atgcatttgg aggatttaag gtacaaggaa aggacaagga agctgctaaa
      481 gaattaataa gagctgctaa agctgtagaa aaagcaggag cattcgctgt agtgttagaa
      541 tgtgttccaa ctaagcttgc agagttaatt tctaaagaga tatcaatacc tacaattggt
      601 attggagcag gtgcagggtg tgatggacaa attcttgtat atcaagatat gttggggatg
      661 tatagtgatt ttacccctaa atttgtgaag aaatatgcta atttatcaga agagatgaat
      721 aaagcattta caaaatatat agaggaagta aaagatggag tttttccagg tccagaacat
      781 gggtttaaaa taagtgatga tgttttagaa aaattatatt aaaagggtca agaaatatgg
      841 ttatatttaa aacagtgaat gatgttagaa gtcaagtaaa agagtggaaa aaacaaggct
      901 taaaggtagg attagttcct actatgggat atttacatga aggtcatgaa agtttaatta
      961 gaaaagcatc aaaagaaaat gataaagttg ttgtaagtat ctttgtaaat ccaactcagt
     1021 ttgggaaaaa tgaagattta ggtagttatc caagagactt agaaagagat atagaagtat
     1081 gcacaagagg tagagcaaca gcaatattta atcctgaagt tgaagagatg tattgtgaca
     1141 atgcaagtac ttttgttaat ataactggat taacagaagg tttatgtgga gctagtaggc
     1201 caatacattt tagaggggtt tgcactgttg taagtaaatt atttaacata ataccagcag
     1261 atagagctta ttttggagaa aaggatgcac aacagttagc tgtaattaaa agaatggtta
     1321 gggaccttaa tattgatata gacgttgtag ggtgtccaat tataagggaa gaggatggac
     1381 tagctaaaag ttctagaaac acatacttaa gtttagagga aagatcttca gctactattt
     1441 taaataaatc tttaacttta gctaaggaag ctttaaataa tgga
//
LOCUS       DAZKLD010000255         1455 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.260, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000255 DAZKLD010000000
VERSION     DAZKLD010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1455
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.260"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..572)
                     /locus_tag="ACG8KR_002937"
     CDS             complement(<1..572)
                     /locus_tag="ACG8KR_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphoryl diester phosphodiesterase
                     membrane domain-containing protein"
                     /protein_id="HFQ0202225.1"
                     /translation="MNYLKRSLGNAWDFLKGTQAYFRDVLLMHGFILFICLPLLSSTT
                     RFILHRGAIDYLSYDNIPSIFSQHPGVLLSLIVVLMTILLLVYFEFTFLLMSVFFIKK
                     KEPISLKQLLHLTILQLKKVRPITFLFFLAYFLLILPISGLSFNSDLLSKIKIPAFIM
                     DFIFANRWIIVSSFLLVYIFLGYIGIRLIFA"
     gene            complement(696..1292)
                     /locus_tag="ACG8KR_002938"
     CDS             complement(696..1292)
                     /locus_tag="ACG8KR_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFQ0202226.1"
                     /translation="MNIAASRKLINFISIIGLGLSIALTIYFINLGVFKDLNALRGLV
                     GDSIILGPIIFVFIQILQVVIPIIPGGISTAAGVLIFGPYAGFIYNYIGICIGSIIIF
                     LLGRRYGKPFILSMISDKTYNKYIGWLDNQNRFEKLFALAIFLPVAPDDALCLMAGLT
                     NMSVKRYTLIILIAKPLSIFLYSMALIYGGQYLSGLLG"
BASE COUNT          573 a          261 c          234 g          387 t
ORIGIN      
        1 gcaaaaatca gtcgaatccc aatatagcca aggaaaatat aaacgagtaa aaaggaactg
       61 acgatgatcc aacgattagc aaaaataaaa tccataatga aagcggggat ttttattttt
      121 gacagtagat cagaattaaa acttaatccg ctgatcggta gaatcaataa aaaataagca
      181 aggaaaaaca aaaaagtaat gggtctcact ttcttcagct gaagtatcgt caagtgtaag
      241 agttgcttca aagagattgg ttctttcttt ttaataaaaa aaacactcat caaaagaaaa
      301 gtaaattcga aataaactaa cagtaaaatc gtcatcaaaa ctacgattaa cgagagtaga
      361 accccaggat gttgagaaaa aattgaagga atattgtcat aagacaaata gtcaattgcc
      421 cctcggtgta aaataaaacg ggtcgtactg ctcaataagg gtaaacaaat aaataaaata
      481 aagccatgca tcagcaagac atcacggaaa taagcttgtg ttccttttaa aaagtcccaa
      541 gcattaccta agctacgttt caaataattc atcctatcac ctgctcttca cattgtagcg
      601 caaatctaat gaaaaaggaa agggtgcagt gtgttttgca gaccctttcc cagatttaac
      661 attgtcaatc aatgactaat tttacaaaat aaatattatc caagaagacc acttagatat
      721 tgacctccat aaatcaaggc catactatat aagaaaatcg acagtggttt agcaatcaaa
      781 atgattagtg tatatcgttt cactgacatg tttgtcaatc cagccatcaa acataacgca
      841 tcgtcaggag caactggaag aaaaatagct agagcaaaca gcttctcaaa acggttttga
      901 ttatccagcc agccaatata tttgttatat gtcttatcgc taatcatgga aagtatgaat
      961 ggctttccat aacgacgccc tagcagaaaa atgataatcg aaccaatgca gattccaata
     1021 tagttataga taaatccagc ataagggcca aatatcagga caccagctgc agtactgatc
     1081 cctccaggaa tgattggaat aactacttgc aggatttgga tgaagacaaa aataatcggt
     1141 cctaaaatga tggaatcacc tactaatcct cttaacgcat tcaaatcttt gaaaacacct
     1201 aaattaataa aataaatcgt cagcgcgatt gataggccta atccgataat ggatatgaaa
     1261 tttatcagtt ttcgagaggc agcaatattc attattcatc aactccttag aggttctttg
     1321 ttaactatac tttactagaa aagcaactaa ctgcgccatt ttttgatatg acagaaaaca
     1381 aacaattttg taagttcatc ataagtataa aagattgatc tcaaaaaaac atccaactaa
     1441 agacttattt aataa
//
LOCUS       DAZKLD010000256         1421 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.261, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000256 DAZKLD010000000
VERSION     DAZKLD010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1421
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.261"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            78..193
                     /gene="rrf"
                     /locus_tag="ACG8KR_002939"
     rRNA            78..193
                     /gene="rrf"
                     /locus_tag="ACG8KR_002939"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            203..276
                     /locus_tag="ACG8KR_002940"
     tRNA            203..276
                     /locus_tag="ACG8KR_002940"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:237..239,aa:Asn,seq:gtt)
     gene            316..405
                     /locus_tag="ACG8KR_002941"
     tRNA            316..405
                     /locus_tag="ACG8KR_002941"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:350..352,aa:Ser,seq:gga)
     gene            420..491
                     /locus_tag="ACG8KR_002942"
     tRNA            420..491
                     /locus_tag="ACG8KR_002942"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:453..455,aa:Glu,seq:ttc)
     gene            507..579
                     /locus_tag="ACG8KR_002943"
     tRNA            507..579
                     /locus_tag="ACG8KR_002943"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:540..542,aa:Val,seq:tac)
     gene            589..661
                     /locus_tag="ACG8KR_002944"
     tRNA            589..661
                     /locus_tag="ACG8KR_002944"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:622..624,aa:Asp,seq:gtc)
     gene            697..772
                     /locus_tag="ACG8KR_002945"
     tRNA            697..772
                     /locus_tag="ACG8KR_002945"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:730..732,aa:Phe,seq:gaa)
     gene            774..854
                     /locus_tag="ACG8KR_002946"
     tRNA            774..854
                     /locus_tag="ACG8KR_002946"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:808..810,aa:Tyr,seq:gta)
     gene            861..934
                     /locus_tag="ACG8KR_002947"
     tRNA            861..934
                     /locus_tag="ACG8KR_002947"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:893..895,aa:Trp,seq:cca)
     gene            937..1009
                     /locus_tag="ACG8KR_002948"
     tRNA            937..1009
                     /locus_tag="ACG8KR_002948"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:970..972,aa:His,seq:gtg)
     gene            1021..1092
                     /locus_tag="ACG8KR_002949"
     tRNA            1021..1092
                     /locus_tag="ACG8KR_002949"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1053..1055,aa:Gln,seq:ttg)
     gene            1138..1208
                     /locus_tag="ACG8KR_002950"
     tRNA            1138..1208
                     /locus_tag="ACG8KR_002950"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1170..1172,aa:Cys,seq:gca)
     gene            1229..1313
                     /locus_tag="ACG8KR_002951"
     tRNA            1229..1313
                     /locus_tag="ACG8KR_002951"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1263..1265,aa:Leu,seq:caa)
BASE COUNT          340 a          315 c          373 g          393 t
ORIGIN      
        1 actcggaaaa aaacttatgt gacttcaaat ccagttttga gtgaacaaaa tttactcaat
       61 aaaagataac accacagtgt ggtggcgata gcgagaagga tacacctgta accatgccga
      121 acacagaagt taagcttctt agcgccgatt gtagtgaggg gttgcccctt gtgagagtag
      181 gacgtcgcca cgctgatgtt attccggcat agctcagttg gtagtagcgc atgactgtta
      241 atcatgatgt cgtaggttcg agtcctactg ccggagttct cattttgaga aagagtattg
      301 agcttctttc actctggaga gttgtccgag aggccgaagg agcatgattg gaaatcatgt
      361 agatggtgcc aaactgtctc aagggttcga atcccttact ctccgtagca aatttacctg
      421 gcccgttggt caagcggtta agacaccgcc ctttcacggc ggtaacacgg gttcgaatcc
      481 cgtacgggtc attttaattg aatgttggag gtttagctca gctgggagag catctgcctt
      541 acaagcagag ggtcagcggt tcgatcccgt taacctccat aatgaactgg ttccgtggtg
      601 taggggttaa catgcctgcc tgtcacgcag gagatcgcgg gttcgattcc cgtcggaacc
      661 gtcgttcttg ttattcaatt aataatttta aaatatggcg cggtagctca gttggtagag
      721 caacggattg aagctccgtg tgtcggcagt tcgattctgt cccgcgccac catggaggag
      781 tagcgaagtg gctaaacgcg acggactgta aatccgttcc ttagggttca gtggttcgaa
      841 tccactctcc tccattttat aggggcatag tttaaaggta gaacagcggt ctccaaaacc
      901 gttagtgtgg gttcaattcc tgctgcccct gccatggcga atgtggcgaa gtggttaacg
      961 caccggattg tggctccggc attcgtgggt tcgattccca tcattcgccc tttttattct
     1021 tggggtatag ccaagcggta aggcaaggga ctttgactcc ctcatgcgtt ggttcgaatc
     1081 cagctacccc agtttctata atttattctt tattttatag attgataata gtataatggc
     1141 ggtatagcca agtggtaagg cagaggtctg caaaaccttc atcaccggtt caaatccggt
     1201 taccgcctta gtactaataa atcaacttgc cggcgtggcg gaattggcag acgcgctgga
     1261 ctcaaaatcc agtgcccgtt gagggcgtgc cggttcgacc ccggccgccg gtattgacta
     1321 agatgtcttc atcttagttt tttattgaga aattaaataa catcttttat gatggcgaat
     1381 gtggcgaagt ggttaacgca ccggattgtg gctccggcat t
//
LOCUS       DAZKLD010000257         1417 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.262, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000257 DAZKLD010000000
VERSION     DAZKLD010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1417
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.262"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(150..1337)
                     /locus_tag="ACG8KR_002952"
     CDS             complement(150..1337)
                     /locus_tag="ACG8KR_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS16 family transposase"
                     /protein_id="HFQ0202227.1"
                     /translation="MTQVHFTFESEEIQAIINESGANDTAKTLMTIMFNQLMEEQRNQ
                     YIQANAYERSEERQSQRNGYYDRSFTTRIGTLELHVPRTRDGKFSPTIFERYQRSEKA
                     LIAAMIEMVISGVSTRKVTKTVELLTDGATVSKSFVSNLMKQLDPFVFEWRNRSLEGS
                     EYPFFMCDALYMKVRENHRIVSKGVYIGIGIDSDGRRTILGFDVQDGESEDNWDTVFQ
                     SFVQRGLFGVKLVISDAHKGLVKAVRKNFLGASWQRCQAHFLRNIFDKLPKKVSSDVK
                     DELKSIFKASELELTRERKEHFLEKYGCDSKLSAACDILENGFEDAIQILSFPENIRR
                     RIRTTNVLERLNEEIRRRERVIRIFPNINSITRIIGTLLMEKDTEWLASPRKYLEFNS
                     NNI"
BASE COUNT          412 a          308 c          249 g          448 t
ORIGIN      
        1 taaaagttca acctcatcaa aagcacaatg ttttgcgttg tgaacctaac tgccatgcct
       61 ttctttctga ggagagaggt caggagcagt agcgcagcga ctcttgatgt ctctcctcag
      121 aaagaagaga ctcgcacagg ttcatggtgc taaatgttat tcgaattaaa ttctaaatat
      181 tttcgtggag aagccagcca ctcagtgtct ttttccatca aaagtgttcc aattatccga
      241 gtgatggaat tgatatttgg aaaaatccga atcactcgtt ctctccggcg aatctcttcg
      301 ttcaaccgtt ccaacacatt ggttgtacga atccttcggc ggatattttc aggaaaagac
      361 aaaatttgaa tagcatcttc aaagccgttt tctaaaatgt cgcaagcagc acttaatttt
      421 gaatcacaac catatttttc caagaagtgc tcttttcttt cacgtgtcaa ttcaagttca
      481 gaggctttaa agatactctt caactcgtct tttacatcag aagaaacttt ctttggcagc
      541 ttatcaaaaa tatttctcaa aaagtgggct tggcatcttt gccagctcgc acctaaaaag
      601 ttcttgcgaa cagccttaac taaccctttg tgtgcatcag aaatgaccag ttttacaccg
      661 aacaaacctc gttgaacgaa cgattgaaaa acggtatccc aattatcttc cgattcgccg
      721 tcttgaacat caaaaccaag aatcgtacgt cgtccatcag aatcaatgcc gataccaata
      781 tatacacctt ttgagacaat acgatgattt tcccttactt tcatatacag ggcatcacac
      841 atgaaaaatg gatattccga gccttctagg ctccggtttc tccactcaaa aacaaatgga
      901 tccaactgtt tcatcagatt tgaaacgaat gatttagaaa cagtcgcacc atccgttagc
      961 agttcaactg ttttagttac tttgcgagtg gagacaccag aaatgaccat ttcaatcatg
     1021 gcagcgatca aagctttttc gcttcgctga tatctttcaa agatagtagg actaaactta
     1081 ccgtctctgg ttcgtggaac atgaagttct aacgtaccaa ttcgagttgt aaacgaacga
     1141 tcgtagtaac catttctttg tgattgccgt tcttctgaac gttcataggc atttgcctga
     1201 atatattgat ttcgttgttc ttccatcagc tgattaaaca tgatagtcat aagtgttttt
     1261 gcggtatcat tcgcaccgct ttcgtttata attgcttgaa tttcttcgct ttcaaaagta
     1321 aaatgtactt gagtcattga tatcagtcct attcgagtat gttttctgca aaaaacattg
     1381 tactcctaac tgagatcaat ggcttttttg atgcttc
//
LOCUS       DAZKLD010000258         1409 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.263, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000258 DAZKLD010000000
VERSION     DAZKLD010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1409
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.263"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1359
                     /locus_tag="ACG8KR_002953"
     CDS             <1..1359
                     /locus_tag="ACG8KR_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HFQ0202228.1"
                     /translation="LFSDEEISVSVPEAAATIIPVKGHQRVVGTKADKIKHLPITEKE
                     HLLPLEEQFCEHCGSQMKDIGRTKVREEIRFHQAMLDCLTHYQHTYCCKSCEKEGLSS
                     FKKAIVPKPLISNSLGSNSLVAETIRMKFGQKVPAYRQENYWKQTHGLDISRDNITNW
                     HIKAVQNALDPLGERLRVYLNQEEILHGDETSYRVIESAKTDTYYWQFCTGKDSQHPI
                     VYYHHDESRAGDVPKTFLKEFTGYLHCDGYSGYNAVESVRLVYCFAHVRRKFFEAIPK
                     GKKNTDIPAAQAVKQLDKWFVLEKKWKDFSPEKRLSCRQQELRPLFIAFYEWMATIDP
                     VAKSKLDAAVQYACKLRSGFEPIFEDGRLELTNNRAERNIKELVIGRKNWLHSTSLEG
                     ARTSGIILSVYKTAELNGLNPVKYLEFLFDKIPNLPVLSAETLDQLLPWNKDVQQHFS
                     RN"
BASE COUNT          466 a          255 c          299 g          389 t
ORIGIN      
        1 ctttttagtg atgaagaaat ttctgtaagt gttccagaag cagctgccac cattattcca
       61 gtaaaaggac atcaacgagt tgtaggaacg aaagcagaca aaatcaaaca cttacctata
      121 acagagaaag aacatctcct tcctttggaa gaacaatttt gtgaacattg cggttcacag
      181 atgaaagata tcggacggac aaaagtacga gaagaaattc gtttccatca ggctatgttg
      241 gattgtctca cccattatca acatacctat tgttgtaaaa gctgcgaaaa agaaggactt
      301 tcttctttta aaaaagcgat cgttcctaaa cctttgattt ctaatagctt aggttccaat
      361 agtttagtgg cggaaacgat ccggatgaaa tttggtcaga aagttcctgc ctatcggcaa
      421 gagaactatt ggaaacaaac acatgggttg gatatttcga gagataatat caccaattgg
      481 catattaaag cagtgcaaaa cgcgttagac cctttaggag aacgattaag agtctatctt
      541 aatcaagaag agattctaca cggggatgaa acaagttatc gtgtgattga aagtgccaaa
      601 acagatacct attactggca gttttgtaca ggaaaagaca gtcagcatcc catcgtttat
      661 tatcaccacg acgaaagtcg tgctggagat gttccgaaaa cttttctcaa ggagtttaca
      721 gggtatttac actgtgacgg ctatagcggg tataatgccg tggaaagtgt acgacttgtc
      781 tattgtttcg cgcatgttcg gcggaaattt ttcgaagcaa ttccaaaagg aaagaaaaac
      841 acggatattc ccgcagctca agcagtcaaa caattggata aatggtttgt cctagaaaag
      901 aaatggaaag acttttctcc tgagaagcga ttaagctgtc ggcaacaaga actgcgtcca
      961 ttatttattg ctttttatga gtggatggca acgatagatc ctgttgcaaa atccaagttg
     1021 gatgcagccg ttcaatatgc gtgtaaacta cgaagtggtt ttgaacctat ttttgaagat
     1081 ggtcgtttag aactcacaaa taatcgtgcg gagcgaaata tcaaagagct ggtgatagga
     1141 cggaaaaatt ggttgcattc cacaagtctt gaaggagcac gaacttcagg aatcattctg
     1201 agtgtatata aaacagcaga acttaacgga ttgaatcctg taaagtatct agagtttcta
     1261 tttgataaga taccaaatct tcccgtactt tcagcagaaa ctttagatca actgttgcct
     1321 tggaacaagg atgtacaaca acatttttct cgtaactaaa aacgccaaac attcccgatt
     1381 ctattctata ggtcgggtta tttgacgta
//
LOCUS       DAZKLD010000259         1408 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.264, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000259 DAZKLD010000000
VERSION     DAZKLD010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1408
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.264"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            793..1005
                     /locus_tag="ACG8KR_002954"
     CDS             793..1005
                     /locus_tag="ACG8KR_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFQ0202229.1"
                     /translation="MKNRLEELRKQRGIKQEDLASALEVSRQTIGSLENGRYNPSIIL
                     AFKIARYFQMSIEEIFIYEEESEDEK"
     gene            995..1390
                     /locus_tag="ACG8KR_002955"
     CDS             995..1390
                     /locus_tag="ACG8KR_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0202230.1"
                     /translation="MKNKGQIWYIGYIVSLFLIVTILVTDFPKMVDIGLAILFSAVFG
                     VSHVQILHDKMIKNDADYKINVMDERNIIIKEKAGNITNMFNTILLGVATVIFICLDY
                     IIPAIIVGSIIAIQPIILIIISSVLEKKM"
BASE COUNT          491 a          210 c          238 g          469 t
ORIGIN      
        1 tggtttccca tataattgaa ggctgtccta atttttacct actatatgat cataattgat
       61 taactcttat atatttccga aaattatctt gaagatttct attggtatgg agaatatcca
      121 tttcattgcc aataaggata taaattctca aaataacaat cgatgtctgt attaaacaag
      181 cctagaatgg taaatctgat tcgccaaaac cattggattg tatcgatttg ctttacgcga
      241 acgccaccta cccattttat aaaaataaaa tgggcaaaag atctttcttt ttgtcataca
      301 aaaagaagaa aaaaaagaga cgattttctt cttccaaaat cgtccctctt ttttctaaaa
      361 ccatggatga acgaacaaaa aatctctcgt attttgtttg ttcatccact aaaaatatgc
      421 gaacagtatg gtttcattca atcctgttct tacaagtcaa aagcatcgaa tcgtccttgg
      481 acgattcaac aaaaaaagta gtcgtttttg gatgactact aagttctaaa cattcattac
      541 ccaaaatcaa tttggtctaa catcgtttct cgactggctt gatctaatcg gctcttgaca
      601 gggctttttc ttttctgttg atagcagata agaggaaata agaataagtg tgttcatgtg
      661 tcataaaaac agtaactaaa acattaaaat caaaatttat atggaaagct tccttgacat
      721 tttgtgctcg caggagtatt ataaaagcac gaaaggaaag taaactttcc attttttaga
      781 aaggagcatg ttgtgaaaaa tcgtttggaa gaattacgaa aacaaagagg aattaaacaa
      841 gaagatttag cttctgctct tgaagtatct cgtcaaacaa taggttcttt agaaaatgga
      901 cgttataatc cctctattat attggcattt aaaattgcaa ggtactttca aatgagcatt
      961 gaagaaattt ttatctatga ggaggaaagt gaagatgaaa aataagggtc agatttggta
     1021 tatcggttat attgtttcgt tgtttttgat tgtgacaatt ttggttacag attttcctaa
     1081 aatggtagat attggacttg ctattttatt cagtgccgtt tttggcgttt ctcatgttca
     1141 aattttgcat gataaaatga ttaaaaatga tgcagactac aaaatcaatg ttatggacga
     1201 acggaatatc ataatcaaag aaaaagcggg aaatataaca aatatgttta acacaatact
     1261 gttaggggtg gcaacagtta tatttatttg tttagactac atcattccag ctattattgt
     1321 cggaagtatc attgcaatcc agccgattat tttaatcatt atcagtagtg tgcttgagaa
     1381 aaaaatgtag aaataaaggt tcatacaa
//
LOCUS       DAZKLD010000260         1391 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.265, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000260 DAZKLD010000000
VERSION     DAZKLD010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1391)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1391)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1391
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.265"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            278..820
                     /locus_tag="ACG8KR_002956"
     CDS             278..820
                     /locus_tag="ACG8KR_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0202231.1"
                     /translation="MLLKVAIKCYCVKNKGGIIYEKIVPRGACLLLALCLSLSAVGCQ
                     KSNENNKIKEGQSISSEEEGMSINANGKNETFKPSDYTLEAKKEYVYEYLGLKFKLSD
                     KFRNYIADKKIAMLDDQSPIDKELKYAILTFEKMTEEQKNAVIEKMGDGYKNWQNELE
                     RIGTIGIFEKNTSEEKNLKL"
     gene            976..1391
                     /locus_tag="ACG8KR_002957"
                     /pseudo
     CDS             976..1391
                     /locus_tag="ACG8KR_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330699.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
BASE COUNT          551 a          164 c          266 g          410 t
ORIGIN      
        1 catgaaaaat cctccagtgt gttttttatt attctacaca ccttattttt tctgtctagt
       61 ctagtgtagc cgattcaaat tgatgaagca gatcccaaag ggctgcttca tcaatttttt
      121 cgtaagtgtc cgtacactta ctgtgcttgc tcatatatta atcgatcata tactaatcgt
      181 gtaagaacaa ctttaagcat aatacttata ttgttatacc atagtttgga aaaataaaaa
      241 attatatttc atcaacgttt cataaaaggt gtgtagaatg ttattgaaag tagctataaa
      301 atgctattgt gtaaaaaata aaggaggaat aatttatgaa aaaatagtac caagaggagc
      361 atgcctatta cttgcattat gtttgagctt aagcgcagtt gggtgtcaaa aaagtaatga
      421 aaacaataaa ataaaagaag gacaatccat aagttctgaa gaggaaggca tgtcaattaa
      481 tgcaaatggc aaaaatgaaa cttttaagcc atcagattat acattggagg caaagaaaga
      541 atatgtttat gaatatttag ggcttaaatt taaactctca gacaaattta gaaattatat
      601 agctgataaa aagatagcga tgttagatga tcaaagtcct atagataaag aactaaagta
      661 tgctattttg acatttgaga aaatgacaga agaacaaaag aatgcagtta tagaaaagat
      721 gggcgatgga tataaaaatt ggcaaaatga gcttgaaaga attgggacta ttggtatatt
      781 tgaaaaaaat acatcagaag aaaaaaatct aaaattataa aatgtgatac tcatactaaa
      841 ataggagttt caagtgatgg aaaatacgat tgctatttaa gcacaaatag cggagctgaa
      901 agtaatcttc tggatgaatt aaaaagaact gagattcaaa ttatagataa aaaagaacgt
      961 cctaaaaatg gttttgtgct atcagaaaaa actgatttag aaaatacaga agcatttaac
     1021 aaagaatcgg ttaaagattt acgtaaacta ccaacaaagg acataaatgg caaggatttt
     1081 acaagtaaag attttgagaa gtatgatctt actatggtaa atgtatttgc aacttggtgt
     1141 acagcttgtg taaaggaaat tcctgatttg gttgaggttc agaatgaaat gaaaagcaag
     1201 ggtgtaaata tcgttggagt tgttacagat cctgttgatg ataacggtga aaacaaggaa
     1261 gcaattgaaa agtcaaagtt aatacatgaa aagacaaagg cttcatatcc tttcttaatg
     1321 ccagatgtgg aatggcaaca ctttttttga caaaaattat aaggtgttgt gaatgctgcg
     1381 gtgttatggc t
//
LOCUS       DAZKLD010000261         1378 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.266, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000261 DAZKLD010000000
VERSION     DAZKLD010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1378)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1378)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1378
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.266"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(131..1300)
                     /locus_tag="ACG8KR_002958"
     CDS             complement(131..1300)
                     /locus_tag="ACG8KR_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEfa13 family transposase"
                     /protein_id="HFQ0202232.1"
                     /translation="MNDFTTEIMEILINKGDLDDLFRRHLELAINALLQAELTVFLDY
                     EKYDRAGFNSGNSRNGNYSRSFKTEYGELNLVIPRDRNGDFSQQTLPAYKRTNDSLET
                     TIIQLVQKGITMSEISDLIEKMYGHYYKPQTISNMSKIVSEDVSAFKERTLEAKYSVI
                     FMDATHIPLKRQTVSKEAVYIVIGIRLDGTKEVLGFTIAPTESAYVWKEILQDLKDRG
                     LEEVLLVVTDGLSGIDDSIHSVYPNAQFQQCCVHISRNIAHKVRVSDRQEICSDFKLV
                     YQASSKEEANNQIRFMIDKWKKQYPRVVKLLMNPAILTFYNFPPSIRRTIYSTNLIEG
                     FNKQLKKYTKRKEQFPNEESLERFLVSQFNNYNQKFLCRVHKGFKEIHDTLESMF"
BASE COUNT          435 a          230 c          207 g          506 t
ORIGIN      
        1 tttttttaca tttttttata aaaaataacc acccgaaggt ggctgaagaa taagctttta
       61 tttgtaagtt tccagaatag gttgtgtcaa taattatgtg taaatggaaa agcccattct
      121 gttgatcaag ttaaaacatt gattctaatg tatcatgtat ttctttaaaa cctttatgaa
      181 ctcggcatag aaacttttga ttatagttgt tgaattgaga tactagaaat ctctccagag
      241 attcttcatt aggaaattgc tcttttcttt tggtatattt ctttaattgt ttgttaaaac
      301 cttcaatcaa attagttgaa taaatagttc ttctgattga cggagggaaa ttataaaagg
      361 ttaatatagc aggattcatg agcaatttaa ccactcgtgg atactgcttt ttccatttat
      421 ctatcataaa ccttatttga ttatttgctt cttcttttga acttgcttgg taaactaatt
      481 tgaaatcgct acagatttct tgtcgatcac taacacgaac cttatgagcg atatttctag
      541 atatatggac acaacattgt tgaaattgag catttggata aacactatga atactatcgt
      601 caataccact taaaccatct gttacgacta acaaaacctc ttctaaacca cggtctttta
      661 gatcttgaag aatctctttc caaacataag cagattcagt tggagctata gtaaatccta
      721 gaacctcttt agttccatcc aacctgatac caattactat atagacagct tcttttgata
      781 cggtttgtct ttttaaggga atatgagtag catccataaa aatgactgag tatttagctt
      841 ctaaagttct ttctttaaat gccgaaacat cttcagacac aattttactc atatttgaaa
      901 tagtttgtgg tttgtaataa tgaccataca ttttttcgat tagatcagag atttcggaca
      961 tagtaattcc tttttgaact agctgaataa tagtggtttc taaagagtca ttggttcttt
     1021 tataagcagg taaagtttgt tgagaaaagt ctccattcct atctctaggt attactaaat
     1081 tcaattcacc gtactctgtt ttaaaagagc gcgaataatt cccattacgg gaattacctg
     1141 aattaaatcc agctctatca tacttttcat agtcaagaaa taccgttaat tcagcttgta
     1201 ataaagcgtt aatagccaat tctaggtgac gacgaaacaa atcatccaaa tcacctttat
     1261 tgattagtat ttccataatt tctgtagtaa aatcgttcat atggaagtcc tcttttctgt
     1321 gaatgtgttg tggtaactct attctacaga agggacttcc ttttttctat ttacacaa
//
LOCUS       DAZKLD010000262         1330 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.267, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000262 DAZKLD010000000
VERSION     DAZKLD010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1330
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.267"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            287..694
                     /locus_tag="ACG8KR_002959"
     CDS             287..694
                     /locus_tag="ACG8KR_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0202233.1"
                     /translation="MSYRVQLIISEDVESQQFGTNVINKVINPALSINAPLIPTALSF
                     SVTAVVSEIEDTEKIKIVEIEVLNKNEKQIFSTGEVSVSLPPQVNDINFNINARNVLV
                     EEAGEHYAVVKFNGTEIGRQIFDIKVNKPVEKN"
     gene            708..1109
                     /locus_tag="ACG8KR_002960"
     CDS             708..1109
                     /locus_tag="ACG8KR_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0202234.1"
                     /translation="MLDIVPSNTHRSGSLINFAAIFACASVILASPVVVDASSTPKTK
                     NDNQLVITTKNEINTISSNDGKIFDVINTVLKRKAEINIKYDEDLNLYFFVIKTTSEL
                     FSSDYDVLDTLDNVLSDYKYMGKSVVATLGE"
     gene            1111..>1330
                     /locus_tag="ACG8KR_002961"
     CDS             1111..>1330
                     /locus_tag="ACG8KR_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0202235.1"
                     /translation="MFDFNEYLSISKQIESDDKYNSKESYRRTAVSRAYYSAFKKSDE
                     YLKENYDIIYNGSSGKGSHQTVWNLFSTV"
BASE COUNT          500 a          163 c          229 g          438 t
ORIGIN      
        1 tgaggggaag tacatatagt tgttgatata gttgctaata ttgttagcta atttgttagt
       61 aaaactgtat acgaatttag atagtaaaaa aacagtttgt tccttatata gaggcacttt
      121 tactactgat taaatgtcag atatgcttac aaatatactt ataaatatgc ttacaaaatt
      181 agctaatatt tccgttgaca aagctttgtt actattgtta aactaaattg actagcaaaa
      241 gtgaaacata aatttattaa ataaatgtaa aaattaggag aaatcaatgt cttatagagt
      301 acaattaata atttcagaag atgtagaaag tcagcaattt ggcactaatg ttattaataa
      361 agtaattaat ccagctcttt ctattaatgc gcctttaatt cctactgctt tatcattttc
      421 tgtaacagct gtggtgtctg agatagagga tacagaaaag ataaaaatag tagaaattga
      481 agttttaaac aagaatgaaa aacaaatttt ttctacgggt gaagtatcgg taagcttgcc
      541 tcctcaagtt aacgatataa actttaatat aaacgccaga aatgtcttgg ttgaagaagc
      601 aggagagcat tatgctgttg ttaaatttaa tggaactgag attggaaggc aaatctttga
      661 tatcaaggtc aacaaaccag tggaaaaaaa ttaaggagac gatacggatg cttgatatag
      721 taccatcaaa tacacatcgg agtggtagct taataaattt tgctgcgatt tttgcttgcg
      781 catcggtaat tctagcatct ccagttgttg tggatgcaag ttctactccg aaaactaaaa
      841 atgataatca acttgtaatt actacaaaaa atgagattaa cacaatttca agtaatgatg
      901 gcaaaatttt tgatgtgata aatacggttt taaaaagaaa agctgaaatc aatataaaat
      961 atgatgaaga tttaaacttg tatttctttg ttatcaaaac aacatctgaa ttgttttcct
     1021 cagattacga tgttttggat actctcgata atgtattatc tgactataaa tatatgggga
     1081 aatctgtagt agcaactttg ggggaataaa atgtttgact ttaatgaata tttatctatt
     1141 tccaaacaaa ttgaatcaga tgataaatat aattctaaag aatcttatag aagaactgct
     1201 gtttcaagag catactacag tgcttttaaa aaatctgatg agtatttgaa agaaaattat
     1261 gatattattt ataatggaag tagcggaaaa ggaagccacc aaaccgtttg gaatttgttt
     1321 tcaacagtta
//
LOCUS       DAZKLD010000263         1316 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.268, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000263 DAZKLD010000000
VERSION     DAZKLD010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1316)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1316)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1316
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.268"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            82..>1316
                     /locus_tag="ACG8KR_002962"
     CDS             82..>1316
                     /locus_tag="ACG8KR_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa11 family transposase"
                     /protein_id="HFQ0202236.1"
                     /translation="MNDSIKKMLRITEKDLMITEVSYETLQKKKTLVVDAVLSPTPRA
                     CRSCGSTVVDGNGKAIIVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKSCQVSLTTVIRTLKEFKSYLPKQSK
                     KILPRVLMVDEFRSHASIEDKMSFICADGETGKLIDVLPTRKLPRLTSYFLGCTNPEE
                     VEFLVTDMNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQAFNELRIREMNELRKAGQKS
                     QAEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLEFSPPLKEAY
                     PFFHELVEAFRDKDPDLFFSLLAELPETLDDSFREKLQNLLTYEEGITNAMIYPYSNG
                     KIEAKNTHIKTMKRVSYGF"
BASE COUNT          446 a          257 c          251 g          362 t
ORIGIN      
        1 attttaggta ttaaaaaaca tatcgttctc tagtatgatt aaatcaccac aaaataaaca
       61 actgagagga cgatacgcta tatgaatgat tctatcaaaa aaatgctgag aataacagag
      121 aaagatttga tgattacaga ggtctcttac gagacccttc agaagaaaaa gacgttggtc
      181 gtcgatgctg ttctctcgcc tactcctcgt gcttgtagaa gttgtggttc tactgtggta
      241 gatggaaacg ggaaagcaat tatagtgaaa aatggaaaaa aggaaacgat tgtccgtttt
      301 gaacaataca atcatatgcc tttggttatg cgcctaaaaa agcaacgcta tacctgtaaa
      361 aactgtcgca cccattggac ggctcaaagt tattttgtcc aacccagaca ttcaatcgca
      421 aatcatgtta gatataaaat tgcttcttta ctgactgaaa aagtatcttt atcttttatt
      481 gcgaaaagct gtcaggtatc tttgaccacc gttattcgta cattgaaaga gtttaaaagc
      541 tatttaccaa agcaatctaa gaagatcctc cccagagtat tgatggttga cgaatttcgt
      601 tcgcatgctt ccatagaaga taagatgagc tttatttgcg cggatggcga aacaggaaaa
      661 ttaatagatg ttttgcctac gcgtaaatta cctcgattaa caagctattt cttaggttgt
      721 accaatccag aagaagtaga attcttggtg acagacatga acgccgccta cttccagctc
      781 accaaacgtg ttctgccaaa tgcgaaagtc gtgattgatc ggtttcatat tgtcaaacac
      841 atgaatcaag cgttcaatga gttgcgtatc cgtgaaatga atgaacttcg taaagccgga
      901 cagaaaagcc aggcagaaaa actgaaaaag aactggcgct ttttgctaaa aaatcgtgca
      961 aacatcaacc attatgaata caaaacatgg aaaagtttcc gagcaccaaa atacccattt
     1021 cttactgaag caatgatgat tgatcgattg cttgagtttt ctccgcccct aaaggaggcg
     1081 tatccctttt ttcatgaatt agttgaagcc tttcgagaca aagaccctga cttatttttc
     1141 tccttattgg cagaacttcc cgaaacgtta gatgacagct ttcgggaaaa gcttcaaaac
     1201 cttctgacct atgaagaagg catcaccaac gcaatgatct atccttattc caatggaaaa
     1261 atagaagcga agaataccca cataaagaca atgaaacgag tatcctacgg atttaa
//
LOCUS       DAZKLD010000264         1282 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.269, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000264 DAZKLD010000000
VERSION     DAZKLD010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1282
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.269"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1201
                     /locus_tag="ACG8KR_002963"
     CDS             <1..1201
                     /locus_tag="ACG8KR_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFQ0202237.1"
                     /translation="VVDAVLSPTPRACRSCGSTVVDGNGKAIIVKNGKKETIVRFEQY
                     NHMPLVTRLKKQRYTCKNCRTHWTAQSYFVRLSHSIADHVRYKIASLLTEKVSLSFIA
                     KSCQVSLTTVTRTLKEFKSYLPKPSKKILPRVLMVDEFRSHASIEDKMSFICADGETG
                     NLIAVLPTRKLPRLTSYFLGCTNPEEVEFLVTDMNAAYFQLTKRVLPNAKVVIDRFHI
                     VKHMNQALNELRIREMNELRKAGQKSQAEKLKKNWRFLLKNRASINHYEYKTWKSFRA
                     PKYPFLTEAMMIDRLLEFSAPLKEAYPFFHELVEAFRDKDPDLFFSLLAELPETLDDG
                     FREKLQNLLTYEEGITSAMIYPYSNGKIEAKNTHIKTMKRVSYGFKSFENMRIRIFLI
                     NQLINVR"
BASE COUNT          410 a          257 c          251 g          364 t
ORIGIN      
        1 ggtcgtcgat gctgttctct cgcctactcc tcgtgcttgt agaagttgtg gttctactgt
       61 ggtagatgga aacgggaaag caattatagt gaaaaatggg aagaaggaaa cgattgtccg
      121 ttttgaacaa tacaatcata tgcctttggt tacgcgccta aaaaagcaac gctatacctg
      181 taaaaactgt cgtacccatt ggacggctca aagttatttt gtccgactca gccattcaat
      241 cgcagatcat gttcgatata aaattgcttc tttactgact gaaaaagtat ctttgtcttt
      301 tattgcgaaa agttgtcagg tatctttgac taccgttact cgtacattga aagagtttaa
      361 aagctattta ccaaagcctt ctaagaagat tctccccaga gtattgatgg ttgatgaatt
      421 tcgttcgcac gcttccatag aagataaaat gagctttatt tgcgcagatg gtgaaacagg
      481 aaacttaatt gctgtcttgc ctacgcgtaa attacctcga ttaacaagct atttcttagg
      541 ctgcaccaat ccagaagaag tagaattctt agtcacagac atgaacgccg cttacttcca
      601 gcttaccaaa cgtgttctgc caaatgcgaa agtagtgatt gatcggtttc atattgtcaa
      661 acacatgaat caggcactca atgagttgcg tatccgtgaa atgaatgagc ttcgtaaagc
      721 aggacagaaa agtcaggcag aaaaactgaa aaagaactgg cgctttctgc taaaaaatcg
      781 tgcaagcatc aaccattatg agtacaaaac atggaaaagt ttccgagcac caaaataccc
      841 atttctcact gaagcaatga tgattgatcg attgcttgag ttttctgcgc ctctaaagga
      901 ggcgtatccc ttttttcatg aattagtcga agcctttcga gacaaagacc ctgacttatt
      961 tttctcctta ttggcagaac ttcccgaaac gttagatgac ggctttcggg agaagcttca
     1021 aaaccttctg acctatgaag aaggtatcac cagcgcaatg atctatcctt attccaatgg
     1081 aaaaattgaa gcgaagaata cccacataaa gacaatgaaa cgagtatcct acggatttaa
     1141 atcattcgag aacatgagaa ttagaatctt tttgatcaat caattaatca atgtaagata
     1201 acaaaaaatc tgaaccagaa tgtggttcat tctgacccgg atttgatttt gcacaactca
     1261 tcagtcctta ttgacaaaga gc
//
LOCUS       DAZKLD010000265         1277 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.270, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000265 DAZKLD010000000
VERSION     DAZKLD010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1277)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1277
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.270"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..701
                     /locus_tag="ACG8KR_002964"
     CDS             <1..701
                     /locus_tag="ACG8KR_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
                     /protein_id="HFQ0202238.1"
                     /translation="KELQREIMPEIIEKLITKEMQVADFNIGDKTWQKTFQMMSVITK
                     NFPSNIPNNYLEYYLYPDMVVEHTDKEYTRANMVMDGREKNTKEMADKIRRGIVEEVL
                     NFNFGEHGQYIVDIAISLLNDDRRRFMLIVPNQGAIPNLRSDAVVEIPAYVGATGVEP
                     ITLRKPISDFHKGLMEAQVAVEKLLVDAYFEGSYQKALQAFTLNQTVPNARVAKKILD
                     EMIKVNKKYWPALK"
     gene            714..1172
                     /locus_tag="ACG8KR_002965"
     CDS             714..1172
                     /locus_tag="ACG8KR_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFQ0202239.1"
                     /translation="MHFFKTRNILKAVASGQIIPLTAVKDEVFSSKMIGVGYAITNHD
                     GYIHAPVKGIIDNIFPTRHAITIESESGRKILIHMGLDTVELEGSPFQIRVESNQEVK
                     EGTLLAIIDLKKLEQAQKDTVIIVVSPGIFEGNLLKQNQEVKIKDSCFEF"
BASE COUNT          474 a          179 c          260 g          364 t
ORIGIN      
        1 ataaagaatt gcaacgagaa attatgccag agattattga aaaactaata acaaaagaaa
       61 tgcaagtcgc cgattttaat ataggagata aaacttggca aaaaaccttt caaatgatgt
      121 cagtcatcac gaaaaatttt ccaagtaata tcccgaataa ttacttagag tattacttat
      181 atcctgatat ggtcgttgag catactgaca aagagtatac acgtgctaat atggtcatgg
      241 atggacgaga gaaaaatacc aaagaaatgg ctgataaaat acgccgcggt attgtggaag
      301 aagtattgaa ctttaatttt ggggagcatg gacaatatat cgtcgatata gctatttcct
      361 tgttgaatga tgatcgtcgt cgctttatgc taatcgttcc taatcaaggg gcgattccga
      421 atctacggtc agatgcagtt gttgaaatac cggcatatgt tggtgcaact ggtgtggagc
      481 caattacact aagaaaacca atcagtgatt ttcataaagg attgatggag gcacaagttg
      541 cagtagagaa gttactagta gatgcttact ttgaaggttc ttatcaaaaa gctttacaag
      601 catttacatt gaatcaaacc gtaccaaatg ccagagtcgc aaaaaaaata ctagatgaaa
      661 tgatcaaagt aaacaaaaaa tattggccag cattaaagta gaggtgataa aaagtgcatt
      721 tctttaaaac aagaaacatt ctaaaagctg tagctagtgg tcaaattatt ccattaacag
      781 cagtaaaaga cgaggtcttt tcttcaaaaa tgataggagt tggatacgct ataacgaatc
      841 atgatggata tatccatgcg ccagttaaag gaataatcga taatatattt cctacccgac
      901 atgccataac gatcgaaagt gaatctggca gaaaaatact gatccatatg ggtttagata
      961 cagttgaact cgaaggaagt ccgtttcaga tacgggttga aagtaaccag gaggtaaaag
     1021 aagggacact gttagctatt atagatttga agaagttgga gcaagcacaa aaagatacgg
     1081 tgatcattgt agtttctcca ggcatttttg aaggaaattt attaaaacaa aaccaagaag
     1141 taaagattaa agatagttgt tttgaatttt agtgtacgat gaaaaaagtt aaatagggtg
     1201 atatttatgg gtgaatttac aaaatttttt tgaaatgata tgtttcaatc actagattta
     1261 atcattttga tttttgt
//
LOCUS       DAZKLD010000266         1274 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.272, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000266 DAZKLD010000000
VERSION     DAZKLD010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1274)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1274)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1274
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.272"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1059
                     /locus_tag="ACG8KR_002966"
     CDS             <1..1059
                     /locus_tag="ACG8KR_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0202240.1"
                     /translation="EKLKEIEEKIIQYDGLAKLKDAKVVSGEQRINREDLSDEFKNVV
                     SLEATNNTKRNILFSSGVFTIKEGKNIEENDKNSIIVHEEFAKQNNLKLGDEVDLELL
                     DIEKSGKIKSHKFKIIGIFSGKKQETYTGLSSDFSENMVFVDYSTSQEILNNSENNEI
                     ANKILMYSGSAESTDLALNKLKELKIDESKYFVEKDSNAFEESLESVSGIKYIIKVMT
                     YSIMLGGMVVLSLILILWLRERIYEIGIFLSIGTSKIQIIMQFIFELIFISIPSIISS
                     LFLGNVLLKVIVDGFINSEDSMISGGSLINNSSFMSNITTLGQSYLILISIIVLSVVF
                     ASSLMLIKKPKEILSKIS"
     gene            1069..>1274
                     /locus_tag="ACG8KR_002967"
     CDS             1069..>1274
                     /locus_tag="ACG8KR_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFQ0202241.1"
                     /translation="MDILEIKNVAYSYANSKEKVLSGVNQKFELGKFYAIVGKSGTGK
                     STLLSLLAGLDKPQTGKILFKNED"
BASE COUNT          527 a          115 c          215 g          417 t
ORIGIN      
        1 gaaaaattaa aagaaattga agaaaaaata attcaatatg atggattagc aaaactaaaa
       61 gatgctaaag tagttagcgg agaacaaaga ataaatagag aagatttatc tgacgaattt
      121 aagaatgttg tttcactcga agctacaaat aatactaaaa gaaatatttt atttagtagt
      181 ggagtattta caattaaaga aggtaaaaat atagaagaaa atgataagaa ttcaattatt
      241 gttcatgaag aatttgctaa acaaaacaat ctaaaattag gtgatgaagt tgatcttgaa
      301 ttactagata ttgaaaaaag tggaaaaata aaaagtcata aatttaaaat tatagggatc
      361 ttttctggta aaaaacagga aacatataca ggattatctt ctgattttag tgaaaatatg
      421 gtgtttgtag attattcaac aagccaagag atattaaaca attcagaaaa taatgaaatt
      481 gcaaataaaa ttttaatgta ttctggtagt gcagaatcta cagacttagc cttaaacaaa
      541 ttgaaagagc ttaaaattga tgagtcaaag tattttgttg aaaaagattc taatgcgttt
      601 gaagagtctt tagagtcagt gagtggaata aaatatataa taaaagtaat gacttattct
      661 attatgttgg gtgggatggt tgttctttca ttaatcttga ttctatggtt aagggaaaga
      721 atttatgaaa taggtatatt cttatctatt ggaacatcta agatacaaat tataatgcaa
      781 tttatattcg agttaatatt catatcgata ccaagtataa tatcttcctt atttttagga
      841 aatgtattac taaaagtaat tgtagatgga ttcattaact cagaggattc aatgatttcc
      901 ggtggaagtt taataaataa tagtagtttt atgtcaaata taacaacact tggacaaagt
      961 tatttaatat taataagtat tattgtttta tcagttgtat ttgcttcttc attaatgtta
     1021 atcaagaagc ctaaagaaat cttatcaaaa ataagttagg agaaaataat ggatatatta
     1081 gaaataaaga atgtagcata tagttatgca aattctaaag aaaaagtttt gtcaggagta
     1141 aatcaaaaat ttgaacttgg gaagttttat gcgatagtag gaaagtcagg aacaggaaaa
     1201 tctacacttc tttccttact tgctggactt gataaacctc aaacaggaaa aatattgttt
     1261 aagaatgaag atat
//
LOCUS       DAZKLD010000267         1257 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.273, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000267 DAZKLD010000000
VERSION     DAZKLD010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1257)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1257)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1257
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.273"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            131..505
                     /locus_tag="ACG8KR_002968"
     CDS             131..505
                     /locus_tag="ACG8KR_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HFQ0202242.1"
                     /translation="MYLKITEQAQERLQKYIDEGATVILDLDDGVGEYSKMGVCSLDT
                     SFRLLLLDKEQRKNDYKLQLDSDIGDVYIKDYSKMYMDEHMTLSLDPRLGVFTLESPS
                     GSLDSHVQLVDLRPEKFSGIKQ"
     gene            complement(619..1248)
                     /locus_tag="ACG8KR_002969"
     CDS             complement(619..1248)
                     /locus_tag="ACG8KR_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFQ0202243.1"
                     /translation="MKIVFLHGLGQDEQSWKDVISYLPNSYSCKSLSLFNHLNSTDTA
                     TLDDLTKYIEVQLNQIKEPFILCGLSLGAVIALNYLATPSPYLKKVVLVAPQFESPNR
                     LLLTFQNIYFYCVPSKIFEKLGLSKKQTLTLLRSLETLDCKKQLRTSRLPKLILCGTK
                     DHANKPAAKRLYKLSTNGRLLFIKNGKHELNREKPEIMAQAMRSFIENS"
BASE COUNT          425 a          178 c          226 g          428 t
ORIGIN      
        1 ctacactctg agatgtaaga aatatcataa gatatttctt acatcttttt gttttgagca
       61 ggactttaga taattttatt aatatatgct agcatgcttt tgtaagaaaa agaaagaggg
      121 cgtattagtt atgtatctta aaattacgga acaagcacaa gaaagattac aaaaatatat
      181 tgatgaaggc gcaacagtta tattggattt agatgatggc gttggggaat attctaaaat
      241 gggtgtttgc tctttagata ctagttttcg tttacttctt cttgataaag aacagagaaa
      301 aaatgattat aaactccaat tagatagtga tattggggat gtttatataa aagattattc
      361 aaaaatgtac atggatgaac atatgaccct ttctttagat ccacgtctag gtgtatttac
      421 tcttgaaagc ccaagtggtt ctttagacag tcatgttcaa ttagtagatc tacgaccaga
      481 aaaattctct ggaataaaac aataactttc tctctattgt ccataaaact agtctcaatc
      541 aagctgtatt gaaaaaacaa gacaacattc ttaccatggt aaataaaaag acgacacatt
      601 ttgtcgtctt tttttaaatt aactattttc gataaaagat cgcatagctt gtgccattat
      661 ttctggcttt tctctattca attcatgttt tccgttttta ataaagagta gccggccatt
      721 cgttgaaagt ttatataaac gttttgctgc tggtttattc gcatgatctt tggtaccgca
      781 taaaatcaat ttaggaagtc gtgatgtacg aagctgtttt ttgcagtcta atgtttcaag
      841 tgaacgaagt agggtaaggg tttgtttttt agatagacct aatttttcaa atatcttaga
      901 aggtacgcag taaaaataaa tattctggaa agtaagcaat aagcgattag gagactcaaa
      961 ttgtggagcg actaaaacaa ccttttttaa atatggagaa ggagttgcaa gatagtttaa
     1021 agcaataaca gcgcctaaag ataatccaca taaaataaaa ggttccttta tctgattcaa
     1081 ttgtacttcg atgtatttgg ttaagtcgtc taaagtagca gtgtctgtgc tgtttaaatg
     1141 attgaacaat gaaagacttt tgcaagaata actattgggt agataagaga ttacatcttt
     1201 ccatgattgt tcgtcttggc ctagaccatg caaaaaaaca attttcatat tgctctc
//
LOCUS       DAZKLD010000268         1253 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.274, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000268 DAZKLD010000000
VERSION     DAZKLD010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1253)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1253)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1253
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.274"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            34..906
                     /gene="scrK"
                     /locus_tag="ACG8KR_002970"
     CDS             34..906
                     /gene="scrK"
                     /locus_tag="ACG8KR_002970"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008865 - fructokinase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase ScrK"
                     /protein_id="HFQ0202244.1"
                     /translation="MLYGAIEAGGTKFVCAIGDEEMTIKERVSFPTTTPEETMPLVID
                     FFKQYQADLAGIGIGSFGPIDIHRDSATYGYITSTPKLAWQNFDFIGTMKKEFPIPIS
                     WTTDVNAAAYGEYVFGSGKGLSSVVYYTIGTGVGGGALQEGRFIEGFSHPEMGHMLVV
                     PHPKDDFAGSCPFHGNCLEGMAAGPAIENRLGKKGQEVAEDDPYWEIEASYIAQCAYN
                     TTLMFSPDVIIFGGGVMKQRHMLQNVHDAFAKLVNGYVETPELSKYIVTPALEDNAGT
                     LGCLALAREAVLHS"
BASE COUNT          377 a          230 c          301 g          345 t
ORIGIN      
        1 tgatttcatt ctaaaataaa gggagaaatg tctatgctat acggtgcaat tgaagcaggc
       61 ggcacaaaat ttgtgtgtgc cattggcgat gaagaaatga cgatcaagga aagagtgagc
      121 tttccgacaa cgacacctga ggaaacaatg cctttagtca ttgacttctt caaacaatac
      181 caagcagatt tagcaggaat tgggattggc tcatttgggc ctatcgatat ccatcgcgat
      241 tccgccactt atgggtacat tacctcgacc ccgaaattgg catggcaaaa ttttgatttc
      301 atcggtacca tgaaaaaaga atttccgatc ccgatttctt ggacaacaga tgtcaatgcg
      361 gctgcctatg gtgagtatgt cttcggcagt ggcaaaggat tgagcagtgt cgtttactat
      421 acgatcggta cgggtgttgg cggcggtgcc ttacaagaag ggcgctttat cgaagggttt
      481 agtcatccag aaatggggca tatgctcgtc gtccctcatc caaaagatga ctttgctggc
      541 agctgtccat ttcatggcaa ctgtttagaa gggatggccg ctggacccgc catcgaaaac
      601 cgcttaggga aaaaaggaca agaggttgca gaagatgatc cttattggga aatcgaagcc
      661 tcctatattg ctcaatgtgc ctacaataca accttgatgt tttctccaga tgtgatcatt
      721 tttggcggtg gtgtgatgaa acaacgccat atgctgcaaa acgtccacga cgcatttgca
      781 aaattggtca atgggtatgt cgaaacacct gagttaagca aatatatcgt gaccccagca
      841 ttagaagaca acgctggaac gctcggttgt ttggcattgg ctcgagaagc ggtcttacat
      901 tcataaacga aaaaaggctc ttcgacattt agtgttggta gagaaagata caactgaaaa
      961 tttagtcata accagttagg cagagtgctt aactggtttt tgttttactt ggaagatcaa
     1021 gccttgaatg agataaattc ttgctcgaaa gttatggaaa tttcgatagc cgtaggccac
     1081 ttgtattgtg caatagtttt ttacaaaaaa aagggggtgc ggatattttt ttggaccttt
     1141 ttaacaaata ggttctgttg caaagtttta aatctactat cagataaggt agaataatag
     1201 aaaaagatag caggaggaat gacgatgagt cattttaaag gaaagcaatt tca
//
LOCUS       DAZKLD010000269         1225 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.275, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000269 DAZKLD010000000
VERSION     DAZKLD010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1225)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1225)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1225
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.275"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            260..460
                     /locus_tag="ACG8KR_002971"
     CDS             260..460
                     /locus_tag="ACG8KR_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0202245.1"
                     /translation="MLKRIAWDKDNKTDIELKEYKYNYFSRCIDRKLLFKKEGLEFSF
                     DEDEFEKLVMDWRKNLLDTVQF"
     gene            651..>1225
                     /locus_tag="ACG8KR_002972"
     CDS             651..>1225
                     /locus_tag="ACG8KR_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0202246.1"
                     /translation="MTKKIEVYVDGSQEERDSFFDFFWKNSREYVKALNLVYKQLMFE
                     ELIKDAIKEMDQGYAEREESIQNRIEKLAYTDPIDQKKLDKAKAELSKLRNEKNKEAR
                     EILTQAIGLKSQTRTRDVAKKLDFDFGDFVDSANMKASKDFGNDFIGIVTGQRTPRIY
                     KNRNKYGLLPFRGRDIKIHKDDKFYIYLPKG"
BASE COUNT          487 a          117 c          228 g          393 t
ORIGIN      
        1 gcttttgatt gaattagtat caagtcttag tagttttgga aaaatgaaaa gatttaatgt
       61 gatattagaa atttttgctt ggttaatccc ttcatttatt tggttttttt atattttcat
      121 agttgaatct gagttaatag ccaaggtctc tcttggactg ttaattccga tataaatagg
      181 ttcagcaatt tactttaata acttgtatac acgtattcaa ataaaaaagc agttattaca
      241 tgaaggatta agtggtaata tgttaaaaag aattgcatgg gataaagata ataaaacaga
      301 tattgagctt aaagaatata aatacaatta tttctcgaga tgtatagata gaaagttact
      361 gtttaaaaaa gaaggattag aatttagttt tgatgaagat gaatttgaaa aattagtgat
      421 ggactggcgt aagaatttac tggatacggt acaattctaa aagcaattga ggtcgaaatt
      481 ccgatacttg aatcatgatc tatattatcg tgttatgagt gaaatttcta ataaagataa
      541 gatatttcat ttggacagtc atgtgaatat gagcttaaca attaaacgta tttatgatct
      601 tttatatgtt tataaacatt aatgaggtgc taaaattgac aacaataact atgacaaaaa
      661 aaatagaagt atatgttgat ggatcacaag aagaaaggga ttcctttttt gatttttttt
      721 ggaaaaatag tagagaatat gtaaaagcgt tgaatctggt atataaacag ctaatgtttg
      781 aagagttgat aaaagatgca attaaagaaa tggatcaagg ttatgctgag agagaagaaa
      841 gtattcaaaa tcggattgaa aagttagcat atactgatcc aatagatcaa aaaaaacttg
      901 ataaagcaaa agctgaatta tctaaattga gaaatgaaaa aaataaagaa gcgagagaaa
      961 tattaacgca agcgataggg ttgaagagtc aaacgagaac acgagatgta gcaaaaaaat
     1021 tagactttga ctttggagat tttgttgatt ccgcaaatat gaaagctagt aaagattttg
     1081 gcaatgattt tatcggaatt gtgaccggac aaagaacgcc tagaatatat aaaaatagaa
     1141 acaaatatgg attactacct tttagaggtc gagatattaa aatccataaa gatgataagt
     1201 tttatattta tttacctaaa ggctt
//
LOCUS       DAZKLD010000270         1209 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.276, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000270 DAZKLD010000000
VERSION     DAZKLD010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1209)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1209)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1209
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.276"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..590
                     /locus_tag="ACG8KR_002973"
     CDS             <1..590
                     /locus_tag="ACG8KR_002973"
                     /inference="COORDINATES: protein motif:HMM:NF015839.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF334 domain-containing protein"
                     /protein_id="HFQ0202247.1"
                     /translation="SEQDRNERTNILMKRLEASTSNFNNKLDVFEEKTKHARLNFETT
                     AKHYIKRLDEDNLKLDFQQAIQDELSDTKDEIREVTKQAREETKEYKEILESKIKDHN
                     KVVDKSNTALKVMTKGVTNIFFVLIIFVLVMLVTGPIGHFFGIEHLYSFINGFIDDHE
                     SAWRYLMLILYAVPYVFFAIILWLIALFFRVFNDL"
     gene            1058..>1209
                     /locus_tag="ACG8KR_002974"
     CDS             1058..>1209
                     /locus_tag="ACG8KR_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HFQ0202248.1"
                     /translation="MNHFKGKQFKKDVIIVAVGYYLRYNLSYREVQELLYDRGINVCH
                     TTIYRWV"
BASE COUNT          454 a          165 c          234 g          356 t
ORIGIN      
        1 agagcgagca agacaggaac gagagaacaa acatactcat gaaaagacta gaggcttcga
       61 cctcgaactt taataataag ttagatgttt ttgaagaaaa gactaagcat gcaagattaa
      121 actttgaaac aactgcaaag cactatatta aacgacttga tgaagataat ttgaaattag
      181 attttcaaca agctattcaa gatgaattaa gcgatactaa agacgaaata cgtgaagtga
      241 cgaaacaagc gagagaagaa acaaaagagt ataaagaaat cttagaaagt aaaataaaag
      301 accataacaa agtggttgat aaatcgaata cagcgctaaa agtgatgaca aaaggcgtta
      361 caaatatatt ttttgtgcta attatttttg tacttgtaat gcttgtgaca ggtccgatag
      421 gtcatttctt tggtattgaa catttatata gctttattaa tggttttatt gatgaccacg
      481 aaagtgcatg gcgatatttg atgttgatac tttatgcagt accctatgta ttcttcgcta
      541 tcattctgtg gctcatagca ttgtttttta gagtatttaa tgacctttag gtagatttat
      601 atataaaaag ggctaaatac gcaaaatacc cccttaaaat gcaaaataaa tgcaaataag
      661 ggggtaactc cttatgaaaa aacaactaat agtattataa cacgttttta tgccgagaaa
      721 acttattggt tggaatgggc tatgtgttag ctaacttgtt agcgagttgg ttggacttga
      781 attgggatta atcccaagaa agtaccaact caacaacaca taaagccctg taggttccga
      841 ccaataagga aattggaata aagcaacgaa aggagttgag taaaatgagt gagaaaacga
      901 agaaaatcat tcgagtatca ttagcttttt cacaagtagt agtgcaatca ttgatagttt
      961 attatactta ccagcaatat aaatggttct gttgcaaagt tttccaaaaa atctatttta
     1021 gtgtaaaatt gagaaaaaag acagagagga cagagtaatg aatcatttta aaggcaaaca
     1081 attcaaaaaa gacgtcatta ttgtcgctgt tggttactac ctgcgttaca atctaagcta
     1141 tcgtgaagtt caggaattgt tatatgatcg tggaataaat gtttgtcata ctacgattta
     1201 tcgttgggt
//
LOCUS       DAZKLD010000271         1172 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.277, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000271 DAZKLD010000000
VERSION     DAZKLD010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1172)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1172)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1172
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.277"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(67..513)
                     /locus_tag="ACG8KR_002975"
     CDS             complement(67..513)
                     /locus_tag="ACG8KR_002975"
                     /inference="COORDINATES: protein motif:HMM:NF013964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFQ0202249.1"
                     /translation="MKIKIYNQEENQTRHIRNLSNAKLEEEARKVSNYNKKMAAKYVN
                     EEEFCFSKTKIYKRNPFVSEYVKRLAKGICQLCQEKGPFIKDGVPYLHCHHIEYLSQG
                     GKDVIENCIALCPNCHARIHELELPDDKEKLLKIVEKRENSFSKIP"
     gene            complement(494..1090)
                     /locus_tag="ACG8KR_002976"
     CDS             complement(494..1090)
                     /locus_tag="ACG8KR_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HFQ0202250.1"
                     /translation="MNPTLITKKELLKKLDISTGVLANLIRNGMPKEGEMFNLDKIIT
                     WRENWSKNILGELEVGRVYTNKEISEKFKCSKQGGMRRSHQTNTLVLFSDQTGSNVYK
                     DKWLNGILQYTGMGLKGDQVLDKNQNKVLANSKSNFVKIHLFETFKPKEHTYLGEVYL
                     AGQIYTVNEKDSSGNSRKVYKFPLALINQEQLIEDKDI"
BASE COUNT          359 a          207 c          136 g          470 t
ORIGIN      
        1 ataatagaac ttagtcttat atagaaggca tagctcacat tagcttattt gtcctataaa
       61 aataaattac ggtattttag aaaagctatt ttctcttttt tcaacgatct tcagcaattt
      121 ttctttatcg tcaggtaact caagttcatg aattctagca tgacaattgg gacataacgc
      181 gatacagttt tcaatcacat cttttccacc ttgcgacaaa tattctatat gatgacaatg
      241 taaataaggt acaccgtctt ttataaatgg ccctttttct tggcaaagtt gacaaatacc
      301 ttttgcaagt cgtttaacat attcagatac gaagggatta cgtttatata tttttgtttt
      361 tgagaagcaa aactcctctt cgttaacata ttttgcagcc atttttttat tataattact
      421 aacttttctt gcttcttcct ctaatttagc attagataaa ttacgaatat gtctagtttg
      481 attttcttcc tggttatata tctttatctt caatcaattg ctcttgattt attaaagcta
      541 atggaaattt atatactttt ctgctgttgc ccgaactatc tttttcatta acagtatata
      601 tttgtcctgc taaatatact tctcccagat atgtgtgttc ttttggttta aatgtttcaa
      661 ataaatgaat ctttacaaaa ttactttttg aattggctaa aactttattt tgattcttat
      721 ccaatacttg atcccctttt agccccatac cggtatattg taaaatacca tttaaccact
      781 tatctttata aacattactt cctgtttgat cactaaacag tacaagagta ttagtttgat
      841 gagatcttct cattccaccc tgtttggaac atttaaactt ctctgaaatt tctttgttag
      901 tatatactct accaacttct aactcaccta atatgttttt agaccaattt tctcgccatg
      961 taataatctt atcaagatta aacatttctc cctcttttgg catgccattt ctaattaaat
     1021 tagctaatac accagtagaa atatctaatt tttttagtaa ttctttctta gtaatcaatg
     1081 ttggattcat aacaatgcag ctcctaatga tatcaatata attgcattag ataacttgaa
     1141 aaatcgaaaa aaactcattt tgtgtcaagt ta
//
LOCUS       DAZKLD010000272         1142 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.278, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000272 DAZKLD010000000
VERSION     DAZKLD010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1142)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1142)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1142
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.278"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(23..1138)
                     /locus_tag="ACG8KR_002977"
     CDS             complement(23..1138)
                     /locus_tag="ACG8KR_002977"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFQ0202251.1"
                     /translation="MGGASIVASKIAKGLIDSGHEVFFVSYLESEKPQVESKYYDLYE
                     KKIFFFKNFSRFHKILEYRLFGGFVPSRYIKKEKEQLKKVLKKEKPDVIIFNTFIPSV
                     LFSEFLKYEFPTVKLITWMHSDPDYSLNHIAKFYKKEYRKSFERVDSIVCLSQDTKEK
                     LSLYNNHIKVIYNPLTLSGSSLISNLDKKIISFTARYDINIKGFDYLCEVAKNLPVGW
                     AIRIAGDGTEEERKELKRLIEKNGVLDKIICAGALFGDELINHYIESSIFISTSRSEG
                     LPLVLIEAMTYGLPVISFDHGGAKEILQDGNYGILIKNFDVQDMNNEIEKLSNNRLLM
                     EKYQKFSLERAKDFNEKKIIGEWQTLLNELGNEDVRN"
BASE COUNT          376 a          199 c          122 g          445 t
ORIGIN      
        1 ttaaaatttt taatcgaagt cctcaatttc taacatcctc atttcctaat tcatttaaca
       61 atgtttgcca ttcaccaatt attttttttt cattaaaatc cttagctctt tctaaagaga
      121 atttttgata tttttccata agtaaacgat tattagacaa tttttctatc tcattattca
      181 tatcttgtac gtcaaaattc ttaattaaaa ttccataatt tccatcttgt aatatttcct
      241 tagcaccacc atgatcaaaa cttattacag gtaatccata agtcattgct tctatcaaca
      301 ctaatggtag tccctcactt ctagaagtag atatgaaaat agaactttct atataatgat
      361 tgattaattc atctccaaat aatgcacctg cgcaaataat tttatctaac acaccatttt
      421 tctcaattaa tcttttcagc tctttacgtt cttcttctgt accatctcca gcaatcctta
      481 tagcccatcc aacaggtaag tttttagcta cttcacataa ataatcaaac cctttaatat
      541 ttatatcgta tctggcagta aatgaaataa tttttttatc aaggttactt attaaactag
      601 agccgcttaa tgtcaatggg ttataaataa ctttaatatg gttattgtat aatgaaagtt
      661 tttcttttgt atcttgcgaa agacaaacaa tggaatccac gcgttcaaaa ctttttctat
      721 attctttttt ataaaattta gcaatatgat ttaagctgta atcaggatca gagtgcatcc
      781 aagtaattaa tttcactgtt ggaaattcat actttaggaa ttcggagaat agtacactag
      841 gtataaaagt attgaatatg atgacatcag gtttttcttt ctttagaact ttttttagtt
      901 gttctttttc tttttttata tatctactag gaacaaagcc tccaaacaat ctatactcta
      961 atatcttatg aaatcgagaa aaatttttaa aaaaaaaaat ttttttttcg tataaatcat
     1021 aatacttaga ttctacttgt ggtttttcac tttctaagta agaaacaaaa aaaacttcat
     1081 ggccactatc tattaatcct tttgcaattt ttgaggccac gatactagct cctcccattg
     1141 ag
//
LOCUS       DAZKLD010000273         1120 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.279, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000273 DAZKLD010000000
VERSION     DAZKLD010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1120)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1120)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1120
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.279"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>1120
                     /locus_tag="ACG8KR_002978"
     CDS             <1..>1120
                     /locus_tag="ACG8KR_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HFQ0202252.1"
                     /translation="TYEFIGIFIGIVGVLIIPLLPLFIKNSTDIPHMYRYYVLFLLNS
                     VLSYFFTYKRSLAIADQKNYLVAINDFAFLFFMNIVQVIALYFYSNFTLFLIVQILFT
                     LFSNINISRTVDKRYSYLKEKDVVSLDKQTKDEVKKNVIGNMSSKIGGVIVMGTDNIL
                     ISTFVNLTAVGIYSNYSLIVTSVQNLCKQITNSITASVGNFAVSNKKKDSFELFKKHF
                     FINHSLIFFSSILLLTLMNPFISLWVGAKYMLPMSTTILIVLNYSVQVYRNTSFVFIE
                     SFGLYWSQRKKPIIEAGVNLLLSLLLLIVFDMGINGVLIGTIGSSLGFVIWYEAFIIY
                     KTVFEKRFIHFFLTFWRSFIELIISAIVVFLLNEYLANG"
BASE COUNT          365 a          138 c          174 g          443 t
ORIGIN      
        1 acatatgaat ttattggtat ttttatcggc atagtaggag tattgattat tccgctgtta
       61 cctctattta ttaaaaatag cacagacata ccacatatgt atcgatatta tgtattattt
      121 ttattaaatt cagttcttag ttattttttc acgtataaaa gatcacttgc aatagcagat
      181 caaaaaaatt atctggttgc gattaatgat tttgcctttt tattctttat gaacattgta
      241 caagtaattg ctttgtactt ttattctaat tttactctat ttctaatagt gcagattttg
      301 tttactctat tttctaatat aaatatttcc agaacagttg acaagagata ttcatattta
      361 aaagagaaag atgtagtttc tctagataag caaacaaaag atgaagtaaa gaaaaatgtc
      421 ataggtaata tgtcctcaaa aataggcggg gttattgtaa tggggacaga taatatatta
      481 atttctacat ttgttaattt aacagctgta ggaatttatt ctaactattc attgattgtc
      541 acaagtgtgc aaaatttatg taagcaaatt actaattcta tcacggcaag tgtaggaaat
      601 tttgctgtat caaataaaaa aaaggattca tttgagctat ttaaaaaaca tttttttatc
      661 aaccactcac taatcttttt ttcttctata ttgttattga ctttaatgaa tcccttcatt
      721 agcttgtggg taggagccaa atacatgcta cctatgtcaa cgactatttt aattgtttta
      781 aattatagtg tacaggttta tagaaataca tccttcgttt ttatagagtc atttggttta
      841 tattggtctc aacggaaaaa gcctataatt gaagcaggtg taaacttgtt attatctttg
      901 cttttgttaa tagtttttga tatgggaatc aatggagtac ttatagggac tatcggatcg
      961 tctctaggat ttgtgatttg gtatgaagca tttatcatat ataaaacggt atttgagaaa
     1021 cgttttattc atttcttttt aactttctgg cgaagtttta tagaattaat aatttcggca
     1081 atagtcgttt ttctattaaa tgaatattta gcaaatggag
//
LOCUS       DAZKLD010000274         1080 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.280, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000274 DAZKLD010000000
VERSION     DAZKLD010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1080)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1080)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1080
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.280"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            289..>1080
                     /locus_tag="ACG8KR_002979"
     CDS             289..>1080
                     /locus_tag="ACG8KR_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0202253.1"
                     /translation="MVKIAGLSKGGKTVSQLYKQNYRDWRQFRKNIKDSYFILPTELE
                     KYLPNNKAINLYLYYCFKAKNEGGDSWHSVNTIAESLGVTTKTINTWNKTLVDLGMIA
                     RIDDNHLSKSTILLPLSSYYKLALDSSLKDVTESTVHEIDGNLVAVYHLFEWRKNEAD
                     ENYNVPYNTLCCVFKRKTQKDTIYKFILVQDEKISNFELSTPSSKIYDDAYRFECQDL
                     EALLSANNLNNIEHENFVVNTRFNLVSEKDSDLLDLLIELTKNLDK"
BASE COUNT          418 a          147 c          174 g          341 t
ORIGIN      
        1 aaaaataaga aattcaaaaa agaaaaaact ttccttaaaa acaaaaaata ggagtattca
       61 gtttcttaat agttttgtaa tagttgtatg tagaagtttt cttaatagtt tttgtaataa
      121 tttattaatc gtagtttata taacttattt gtaagtgact agagaattaa ataaaaaata
      181 attactatct agtacgaaat taattacata ggataatata ttaaggtgct ggactacaag
      241 taaaaaatac tgtatgatca tgacagattc aagaaaggag caataagatt ggttaagata
      301 gcaggacttt ctaaaggtgg aaaaaccgtt tcacaacttt ataaacaaaa ctacagagac
      361 tggagacagt tcagaaaaaa cattaaggat tcttacttta ttctaccaac agaacttgaa
      421 aaatatttac cgaataacaa agctattaat ttatatctgt attactgttt taaggcaaag
      481 aatgaaggcg gggattcctg gcattcggtc aataccatag cagagtctct aggagtgaca
      541 actaaaacta taaacacctg gaataaaacc ttagttgatt taggtatgat tgcacgtata
      601 gacgataacc atctcagtaa atcgacaatt ttacttcctc tctctagtta ctataaatta
      661 gcccttgata gttctttaaa ggatgttacc gagagcactg tccatgaaat tgatggcaac
      721 ttggttgcag tttatcattt gtttgaatgg agaaaaaatg aggctgatga aaattataac
      781 gttccttata acacactctg ttgtgttttt aaaagaaaaa cacaaaaaga tactatctat
      841 aaatttatct tggtccaaga tgagaaaata agtaatttcg aattaagcac tccttctagt
      901 aagatttatg acgatgcata tagatttgaa tgtcaagatc tagaagccct cctttccgca
      961 aataatctaa acaatataga acatgaaaac tttgttgtaa acactagatt taatttagtt
     1021 tctgaaaagg actcagattt gttagatttg ctgatagagc ttactaaaaa tctagataag
//
LOCUS       DAZKLD010000275         1060 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.281, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000275 DAZKLD010000000
VERSION     DAZKLD010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1060)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1060)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1060
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.281"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(38..994)
                     /locus_tag="ACG8KR_002980"
     CDS             complement(38..994)
                     /locus_tag="ACG8KR_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0202254.1"
                     /translation="MELYTLPIIIKTEKMLKDLKQYYPLSAYEKKKLFHFLAINLLAE
                     QADSLDIASNQSLFFSDIGFYHLKGVLGEKSVIFINKTLSWLYLHDKLDQKYLRKNED
                     ETIEWLNERFIESFENQFNLLREETSQLIRRELTKIHFKLLHYTINKADEFPLDVSFF
                     RENYPAFYCYLIEYINDLTSKRKELLQDKFFLFFSYLLLLINHIPIQLISEPVKIIID
                     FSYGAAYNQFIKKNLSLYINLNTEVIEAGKPEFPDVIITNRNNLYEESASQVVVWLDP
                     PRAVDWGNLTKILLTIQEEKYKDSKIDKQIDDFPEEIIDRIE"
BASE COUNT          344 a          176 c          160 g          380 t
ORIGIN      
        1 tttagaaaaa gcagtcggac tttctaaaag aagttcacta ttcgatcctg tcgatgattt
       61 cttcaggaaa atcgtctatt tgcttgtcaa ttttgctgtc tttgtatttc tcttcttgga
      121 tagtcaacaa aatctttgta agattccccc agtctactgc tcttggcgga tcgagccaaa
      181 ccactacttg agatgcactt tcttcatata gattattcct attcgtgatt ataacatccg
      241 ggaactcagg ctttcctgcc tctatcactt ccgtgttcag attaatgtac aagcttaaat
      301 ttttcttgat aaattgatta taggcagcac cataagaaaa atcgatgatg atctttactg
      361 gttcagaaat cagttgaatg ggtatgtgat tgatcaataa taataaataa ctgaaaaaaa
      421 gaaaaaattt atcttgaagc aactctttgc gttttgacgt tagatcattt atatactcta
      481 tcaaatagca ataaaatgct ggatagtttt ctctaaaaaa agaaacatca agtggaaatt
      541 catcagcttt attaatagtg taatgcaaaa gtttaaaatg tattttggtt aactctcttc
      601 ggatcaattg gctcgtctct tctcgcaaca gattaaactg attttcaaaa ctttcaatga
      661 atcgttcatt caaccattcg attgtttcat cttcattttt tctcaagtat ttttgatcca
      721 atttatcatg aagataaagc caagagagtg tcttattgat aaaaataacg gatttttctc
      781 ctaatactcc ttttaagtgg taaaatccta tatccgaaaa aaatagcgac tgattgctag
      841 caatatctaa agaatcagct tgttcagcca acaggttgat tgctaaaaag tggaataatt
      901 tctttttttc ataagcagac agaggataat actgttttaa atcttttaac attttttctg
      961 tttttataat tataggtagc gtatacaatt ccacaaaact tatctctgtt gagttaaaaa
     1021 gattatataa aaaaagccga atgtttttag gattccctgt
//
LOCUS       DAZKLD010000276         1057 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.282, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000276 DAZKLD010000000
VERSION     DAZKLD010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1057)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1057)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1057
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.282"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(170..907)
                     /gene="erm(B)"
                     /locus_tag="ACG8KR_002981"
     CDS             complement(170..907)
                     /gene="erm(B)"
                     /locus_tag="ACG8KR_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011100845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HFQ0202255.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESRASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTITYEQVLSIFNSYLLFNGRK"
BASE COUNT          310 a          170 c          185 g          392 t
ORIGIN      
        1 gggtcccgag cgcttagtgg gaatttgtac cccttatcga tacaaattcc ccgtaggcgc
       61 tagggacctc tttagcttct tggaagctgt cagtagtata tctaataatt tatctccatt
      121 ccctttagta acgtgtaact ttccaaattt aaaaaagcga ctcatagaat tatttcctcc
      181 cgttaaataa tagataacta ttaaaaatag acaatacttg ctcataagta atggtactta
      241 aattgtttac tttggcgtgt ttcattgctt gatgaaactg atttttagta aacagttgac
      301 gatattctcg attgacccat tttgaaacaa agtacgtata tagcttccaa tatttatctg
      361 gaacatctgt ggtatggcgg gtaagtttta ttaagacact gtttactttt ggtttaggat
      421 gaaagcattc cgctggcagc ttaagcaatt gctgaatcga gacttgagtg tgcaagagca
      481 accctagtgt tcggtgaata tccaaggtac gcttgtagaa tccttcttca acaatcagat
      541 agatgtcaga cgcacggctt tcaaaaacca cttttttaat aatttgtgtg cttaaatggt
      601 aaggaatatt cccaacaatt ttatacctct gtttgttagg gaattgaaac tgtagaatat
      661 cttggtgaat taaagtgaca cgagtattca gttttaattt ttctgacgat aagttgaata
      721 gatgactgtc taattcaata gacgttacct gtttacttat tttagccagt ttcgtcgtta
      781 aatgcccttt acctgttcca atttcgtaaa cggtatcggt ttcttttaaa ttcaattgtt
      841 ttattatttg gttgagtact ttttcactcg ttaaaaagtt ttgagaatat tttatatttt
      901 tgttcatgta atctctcctg aagtgattac atctataaac aaatacagaa gttaaacgat
      961 ttgtttgtaa ttttagttat ctgtttaaaa agtcataaga ttagtcactg gtaggaatta
     1021 atctaacgta tttatttatc tgcgtaatca ctgtttt
//
LOCUS       DAZKLD010000277         1053 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.283, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000277 DAZKLD010000000
VERSION     DAZKLD010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1053)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1053)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1053
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.283"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            189..>1053
                     /locus_tag="ACG8KR_002982"
     CDS             189..>1053
                     /locus_tag="ACG8KR_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFQ0202256.1"
                     /translation="MKTKMCRISIIVPVYNVEKYLKKCVDSILGQTFTDFELLLIDDG
                     STDNSGSICDELAKTDNRIKVIHKENGGLSDARNIGIEVAKGDFIGFIDSDDYIDEDM
                     YAFLYNNILKYDAELSMCGIYDVHKNKEIKKLTPFSQLVTKSEAIELVLDGKLVVANA
                     VSKLYKKELFENVRYPKGKIAEDAAVILKIINQCNKIHVDTSQKYYYYHRGDSITSKK
                     FTKKDFDIIDVWNENEKWIIKNYPQMYDKVHTRVCWAYFVVLDKLVLTEEKGLYQEKK
                     QVREYLIDNFFF"
BASE COUNT          433 a           93 c          190 g          337 t
ORIGIN      
        1 cgtaaaaaaa cagtaattga atttttaaag aagaacagaa attttatatt aacttataag
       61 ggattcacta aatcgcgcaa aatagctatt attgctctga gctttagagt agattggtat
      121 aggaaattgg ttgaacaaaa aaataaaaaa aattagaatg tttaaattag taaataaaag
      181 aaaaggaatt gaaaactaaa atgtgcagaa taagtataat agttccagtt tataatgttg
      241 aaaaatattt aaaaaagtgt gtcgattcaa ttctaggcca aacatttacc gattttgaat
      301 tattattaat tgatgatggt tctacagata attcaggaag tatatgcgat gaacttgcga
      361 aaacagacaa ccgaataaaa gtgatacata aggaaaatgg gggtctaagt gatgccagaa
      421 atattggtat tgaggtagca aagggagatt ttattgggtt tatagatagt gatgattaca
      481 ttgatgaaga tatgtatgca tttttatata acaatatatt gaaatatgat gcagagttat
      541 caatgtgtgg tatttatgac gtccataaga ataaagaaat taaaaagtta actccttttt
      601 ctcaattggt tactaaatct gaagcaattg aattagtatt agatggtaag ctagttgttg
      661 ctaatgcagt aagcaagcta tataaaaaag aattatttga gaatgtgaga tatccaaaag
      721 gaaaaattgc tgaagacgca gcagtaatac tgaaaattat caatcagtgt aataaaatcc
      781 atgttgatac ttctcaaaaa tattattatt atcatcgtgg agatagtatt actagtaaaa
      841 aatttacaaa aaaagatttt gatataattg atgtatggaa tgagaatgaa aaatggatta
      901 ttaaaaatta tcctcaaatg tacgataaag tacatactag agtttgttgg gcttactttg
      961 ttgtgttaga taaattagtt ttaactgaag aaaaaggact gtatcaagaa aaaaaacaag
     1021 taagagagta tttaattgat aatttctttt tta
//
LOCUS       DAZKLD010000278         1004 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.285, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000278 DAZKLD010000000
VERSION     DAZKLD010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1004)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1004)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1004
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.285"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            37..432
                     /gene="panD"
                     /locus_tag="ACG8KR_002983"
     CDS             37..432
                     /gene="panD"
                     /locus_tag="ACG8KR_002983"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HFQ0202257.1"
                     /translation="MKVTMLKGKIHRATVEQAELDYVGSITVDMNLLEAAGIYEYEKV
                     QIVDVNNGNRFETYTIAGERGTGMICLNGAAARCVSTGDKIILMAYCELNENEVKDHR
                     PKVVFVDDNNKVERVTSYEKHGRLSDIIL"
     gene            634..1004
                     /locus_tag="ACG8KR_002984"
                     /pseudo
     CDS             634..1004
                     /locus_tag="ACG8KR_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          403 a          129 c          195 g          277 t
ORIGIN      
        1 gataatttta catttgaatt acaatagggg gctataatga aagttacaat gttaaaagga
       61 aaaatacata gagctacagt tgaacaagct gagttagatt atgtaggaag tattactgtt
      121 gatatgaatt tattagaagc agcaggaata tatgaatatg aaaaagtaca aatagttgat
      181 gttaataatg gaaatagatt tgaaacttat acaatagctg gagagagagg aacagggatg
      241 atttgcttga atggagctgc agctagatgt gtatcaactg gagataaaat aattttaatg
      301 gcatattgtg aattaaatga aaatgaggtt aaggatcata gacctaaggt tgtttttgtc
      361 gatgataata ataaagttga aagagtaaca tcatatgaaa aacatggaag gttatcagat
      421 ataattcttt aggttttgta atgttattta tatatagcta gactattatt gaaatattta
      481 ttgattacta attggataga tgtcaataaa gtagacacaa aaagagaaga tacagactgt
      541 agacaaagtc cattttatgt gaatcggcta cactagacta gacagaaaaa ataaggtgtg
      601 tagaataata aaaaacacac tggaggattt ttcatgtcaa gacgtcaacg aagaacctat
      661 tcaaaagaat tcaaacaaca aatcgtcaat ctctatctcg ctggtaagcc tcgcgcagaa
      721 attattcgag agtatgagct tacgccttct tctttcgata aatggatgaa gcaagcacaa
      781 tcaacgggct cattcaaaaa aagagacaac ttaacaccag aacaagcaga attgatcgca
      841 ctaagaaaga aaaataagca actcgaaatg gagaatgata ttttaaagca agcggcgctg
      901 atattcggac gaaaagacaa gtaattgatg ccaacaagca taaatattcc atatcagcga
      961 tgtgcaaaat tctaaatatt tctcgtcaga cctactatta tcaa
//
LOCUS       DAZKLD010000279         1003 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.286, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000279 DAZKLD010000000
VERSION     DAZKLD010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1003
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.286"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            16..>1003
                     /locus_tag="ACG8KR_002985"
     CDS             16..>1003
                     /locus_tag="ACG8KR_002985"
                     /inference="COORDINATES: protein motif:HMM:NF033550.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFQ0202258.1"
                     /translation="MILLNRSGNKRTYLRLKKQRYLCRACKKYFTARTYLVTPFCFIS
                     KQIHYKILEELTERQSIKAIGKHCDVSVTTVQRTLSALSSDIRQNLEYLPRCLLFDEF
                     RSLSTWHGKFSFSCMDGETGKLFEILPSRRKKDLVAFFMRFSLKARLGVHYIVTDMNA
                     PYFSLVKECFPNAQVIVDRFHIVQHLNRFFDAVRKRVMKTLDQKDPIQAKQYRQLKSL
                     FKLLLKSEDRLDYNTLTKRRNFNWRLLTETEVVDRLLKISPELKTAYRYYQDLLTAYQ
                     EKDPDTFFQLLRAMPGEVPLELHHIKKAFFKYEKGIRLALLKKYSNARLENLH"
BASE COUNT          301 a          209 c          191 g          302 t
ORIGIN      
        1 ggcaagaaga tctctatgat tctcctgaat cgtagcggta ataaacggac ctatcttcgg
       61 ttgaagaaac agcgatacct ctgccgagca tgtaaaaaat attttaccgc tcggacgtat
      121 ctcgttaccc cattctgctt tatttctaag cagattcatt acaagatctt agaagaattg
      181 acagagcgtc aatctataaa agcaattggt aaacattgcg atgtttctgt gacaactgtt
      241 cagcggacat tgtccgcctt gtcatcagat atccgtcaga atcttgagta tttacctcgg
      301 tgtctattat tcgatgagtt tcgttctctt tctacatggc acggtaaatt ttctttttct
      361 tgtatggacg gtgagaccgg gaaactgttt gagatcttac ctagccgccg taaaaaagat
      421 ctcgttgctt ttttcatgcg tttttctctc aaagcgaggt tgggtgtaca ttacatcgta
      481 acggatatga acgctcctta tttctcacta gttaaagagt gttttccaaa tgcacaagtg
      541 atcgtcgatc ggtttcacat tgttcaacat ctgaaccgct tcttcgatgc ggttcgaaaa
      601 cgtgtgatga aaacacttga tcaaaaagac ccaatacaag ctaaacagta tcgccaattg
      661 aaatccttat tcaaattact tttaaaatca gaggatcgtt tagattacaa tacactaacg
      721 aaaagacgca atttcaattg gagactattg actgaaacag aagtagttga ccgccttcta
      781 aagatttctc ctgaacttaa aacagcttat cgttactacc aagacctgct tactgcgtat
      841 caggaaaaag atccagatac ttttttccag ctgcttcgag caatgcctgg agaagttcca
      901 ttggaactgc accatatcaa aaaagccttt ttcaaatacg agaaagggat tcggctggca
      961 ttactgaaga agtattcgaa tgcacgactg gaaaatctcc ata
//
LOCUS       DAZKLD010000280          999 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.287, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000280 DAZKLD010000000
VERSION     DAZKLD010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 999)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 999)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..999
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.287"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(81..635)
                     /locus_tag="ACG8KR_002986"
     CDS             complement(81..635)
                     /locus_tag="ACG8KR_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFQ0202259.1"
                     /translation="MSYNVQPLRTNEEIQDFQFWLRRTSNPERDSFLFLFGINNGLRM
                     SDIIGLKVGDIRGKSKPIIVEHKTGKRKPIFIDNLREEILLYTEGKEENDWLFPSRQQ
                     GRHITRDRVYQIYADVAEKLGRDDIGTHTLRKTFGYHYYKKTRDIATLMFIFNHSSQA
                     ITKRYIGITEDEIGASLRGFKLGG"
BASE COUNT          323 a          186 c          107 g          383 t
ORIGIN      
        1 cttccgaaaa ttcagttatc ttcattaatc tcaccttcta agttttcatt atacacaaaa
       61 aatatatcct aatatctcaa ttagcctccc aatttaaaac cacgtaacga tgcccctatt
      121 tcatcttcag ttataccaat gtaccttttt gtaattgctt gcgagctatg attaaaaata
      181 aacattaagg tagcaatgtc tctagttttc ttgtagtagt gatacccaaa agtttttctt
      241 aatgtatgtg ttcctatatc atctcttcct aatttttctg ctacatctgc atatatctga
      301 tagacacgat cccttgttat atgtcgacct tgttgccgac ttggaaataa ccaatcattt
      361 tcttctttac cttcagtata taataaaatc tcttctctca aattatctat aaaaatcggt
      421 tttcgcttac ctgttttatg ttcaacaata ataggcttac ttttacctcg tatgtctcca
      481 acttttaatc ctataatatc tgacatacgt aacccattat tgattccaaa caaaaataaa
      541 aaactatctc tttctggatt acttgttctt cttagccaaa actgaaaatc ttgtatctct
      601 tcatttgtcc ttaacggttg tacattatag ctcactattg tttcccttct taaaaatatt
      661 ctgagttcat gattatatat atattttacc ataatcatga acttatatat atactctgtt
      721 aaaagtgaag aaaatcaagg ggtttagttt atgattttat atcatttcag taactcagaa
      781 tattccgact tttttgtaca aaaaaattaa ccttacttat atgaagattc taagctctta
      841 tttaaatttt agacgggcag ttatccgtag aagttcttag aatagctaat ttacataatt
      901 aaattatatg aaatttaagt ttgaacaata tactaagaac tccccttact ataaagtgaa
      961 ataaattagc atatgaataa ccgtgctacc aaacttttc
//
LOCUS       DAZKLD010000281          979 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.288, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000281 DAZKLD010000000
VERSION     DAZKLD010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 979)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 979)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..979
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.288"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            159..383
                     /locus_tag="ACG8KR_002987"
     CDS             159..383
                     /locus_tag="ACG8KR_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II bacteriocin"
                     /protein_id="HFQ0202260.1"
                     /translation="MKKKVLKHCVILGILGTCLAGIGTGIKVDAATYYGNGLYCNKEK
                     CWVDWNQAKGEIGKIIVNGWVNHGPWAPRR"
     gene            403..690
                     /locus_tag="ACG8KR_002988"
     CDS             403..690
                     /locus_tag="ACG8KR_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFQ0202261.1"
                     /translation="MDFTKEEKLLNAISKVYNEATIDDYPDLKEKLFLYSKEISEGKS
                     VGEVSMKLSSFLGRYILKHKFGLPKSLIELQEIVSKESQVYRGWASIGIWS"
BASE COUNT          376 a          101 c          190 g          312 t
ORIGIN      
        1 taaataactg atcttataat tttatttaaa cgattatgaa ttcaaaacta ctttttatga
       61 cgttaatcat tgttatataa taaaatgtta tataataaaa ttactaagat tatttaatat
      121 aacaagcaat tttaaaaaaa cgaaaaggaa aaacaatcat gaaaaagaaa gtattaaaac
      181 attgtgttat tctaggaata ttaggaactt gtctagctgg catcggtaca ggaataaaag
      241 ttgatgcagc tacttactat ggaaatggtc tttattgtaa caaagaaaaa tgttgggtag
      301 attggaatca agctaaagga gaaattggaa aaattattgt taatggttgg gttaatcatg
      361 gtccatgggc acctagaagg tagtggtagg gaggttatta taatggattt tactaaagaa
      421 gaaaaacttt taaatgcaat tagtaaagta tacaatgaag caactataga tgactatcct
      481 gacttaaaag aaaagctctt tctttattct aaagaaatca gtgagggaaa aagtgttggt
      541 gaagttagta tgaaattaag tagttttctt ggaagatata ttttaaaaca taaatttgga
      601 ttacctaaat ctttaataga attacaagaa attgttagta aggaatctca agtatataga
      661 ggatgggctt ctattggtat ttggagttaa ttctttggaa taaaatgaaa atgggactga
      721 agaatcaatt ctgattcatc agtcccataa attatagagc cgaaaaatta tcatatttat
      781 agatttatta atagtaaatg gcataagaat ctttgaacaa cgaaaaaaat gtagctctac
      841 ctaattattg atttgcaaca atttcaccga aggtgaaaca agcgaaactt tacaaaaagt
      901 ggctagcatt tgctagtctt ttttgtattg tggagagtgg gggggagggg gattcaaggg
      961 ggagcttgcg cccctttga
//
LOCUS       DAZKLD010000282          949 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.289, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000282 DAZKLD010000000
VERSION     DAZKLD010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 949)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 949)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..949
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.289"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            68..388
                     /locus_tag="ACG8KR_002989"
     CDS             68..388
                     /locus_tag="ACG8KR_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFQ0202262.1"
                     /translation="MNTYEIIKDLAKRKKISIRQLEINFGYSNGYLAKWKNNTPNSDE
                     LSRIADYFDVSVDYLLGREERETPKHVDLSEDDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
     gene            406..837
                     /locus_tag="ACG8KR_002990"
     CDS             406..837
                     /locus_tag="ACG8KR_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HFQ0202263.1"
                     /translation="MYLKLKEMLSEYNLKVVYMEMKEPGFYYPKPRIIFLNENLYGET
                     AEAFHLSHELAHFSASHFEFSVLYDTSTTFHSKFETEADKIAILILLNIYIENELTDE
                     SQFNLEKFMEYYSIQNKLRYTCYAVCQCYFKKKYSYARQYV"
BASE COUNT          342 a          139 c          160 g          308 t
ORIGIN      
        1 tagtcaaatt atttctgcgt atttttctaa actttttttg taaacattta gaaaggttgt
       61 tttatcgatg aatacctatg aaataataaa agacttggcg aagaggaaaa agatatctat
      121 acggcagtta gaaattaatt ttggatattc taacggttat ctagcaaagt ggaaaaataa
      181 tactcctaat tcagatgaat taagtcggat tgctgactat ttcgatgtct ctgtagacta
      241 ccttctaggt agagaagaaa gagaaacccc taaacatgtg gatttatcag aagacgatac
      301 tgttttttct tttgatggaa aagaaatatc taaggagaca atgcgtaaag cgattgcaat
      361 tgctaaagct ttagaggaaa atgaatagtt ggagtgatgg tttgtatgta tttgaaattg
      421 aaagaaatgt tgagcgaata caatttgaaa gtagtttata tggagatgaa agagcctggt
      481 ttctactatc caaaacccag aatcattttt ttgaatgaaa atctatacgg tgaaactgca
      541 gaagcctttc atttatctca tgaacttgca catttcagcg cctctcattt tgaattttca
      601 gttttgtacg atacttctac aacttttcac tcaaaatttg aaacagaagc agataaaata
      661 gctattctaa ttttattaaa tatctatatt gagaacgaac tgactgatga atctcagttt
      721 aatctcgaaa aatttatgga gtattattct attcagaata agctcagata cacttgttat
      781 gctgtctgcc aatgctattt taagaaaaaa tattcttacg caaggcaata tgtatgaata
      841 catcaagaat tattaaaaaa accgatttca gcaaaacatt aaaaaagccc gtgctgcaac
      901 acgggctctt tcctcatata tgagcttcta caaaaaaaat catatcaca
//
LOCUS       DAZKLD010000283          943 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.290, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000283 DAZKLD010000000
VERSION     DAZKLD010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..943
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.290"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>943
                     /locus_tag="ACG8KR_002991"
     rRNA            <1..>943
                     /locus_tag="ACG8KR_002991"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          293 a          187 c          274 g          189 t
ORIGIN      
        1 attgtgtgaa tacatagcac attcgaggta gacgcagaga actgaaacat ctaagtacct
       61 gcaggaagag aaagaaaatt cgattccctg agtagcggcg agcgaaacgg gaaaagccca
      121 aaccagcaag cttgcttgtt ggggttgtag gactccaata tggtagttct ttcagatagt
      181 cgaatgactt ggaaaagtca gtcaaagagg gtaaaaaccc cgtagacgaa atgtggaaga
      241 cacctaggag gatcctgagt acggcggaac acgagaaatt ccgtcggaat ccgggaggac
      301 catctcccaa ggctaaatac tccctagtga ccgatagtga accagtaccg tgagggaaag
      361 gtgaaaagca ccccggaagg ggagtgaaat agaacctgaa accgtgtgcc tacaacaagt
      421 caaagcccgt taatgggtga tggcgtgcct tttgtagaat gaaccggcga gttacgattg
      481 catgcgaggt taagttgaag agacggagcc gcagcgaaag cgagtctgaa tagggcgttt
      541 gagtatgtag tcgtagaccc gaaaccatgt gatctaccca tgtccaggtt gaaggtgcgg
      601 taaaacgcac tggaggaccg aacccacgta cgttgaaaag tgcggggatg aggtgtgggt
      661 agcggagaaa ttccaaacga acttggagat agctggttct ctccgaaata gctttagggc
      721 tagcctcgga attgagaatg atggaggtag agcactgttt ggactagggg cccatctcgg
      781 gttaccgaat tcagataaac tccgaatgcc attcattcat atccgggagt cagactgtga
      841 gtgataagat ccatagtcga aagggaaaca gcccagacca ccagctaagg tcccaaaata
      901 tatgttaagt ggaaaaggat gtggggttgc acagacaact agg
//
LOCUS       DAZKLD010000284          922 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.291, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000284 DAZKLD010000000
VERSION     DAZKLD010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 922)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 922)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..922
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.291"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(127..675)
                     /gene="aac(6')"
                     /locus_tag="ACG8KR_002992"
     CDS             complement(127..675)
                     /gene="aac(6')"
                     /locus_tag="ACG8KR_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HFQ0202264.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT          268 a          192 c          138 g          324 t
ORIGIN      
        1 gtacacggtg ttccaaaagt agcttcttcg gaaataagtt ggaatttcac aaaaattcaa
       61 gaagtaattt tcgtaaattc cttcttattt cttgaagctg attacttctg tcacaacctc
      121 tttttattat tgagaatttg gtcgaggaat aatcgttttt gccatccaaa tatccggttt
      181 gtcccagcca tttgcatttg gtaagacacc tacgatttta taacctaatt tttcatagaa
      241 ttcatacgga tgttcacgaa ggttctggat ggaagccact ttatcaaatg tatgctcata
      301 caggtccgtt tgacttaacg ttgttccatg gtctaaatca tccgtaccta aataaatcgt
      361 gattcctcct ctggaagcta cttctttttc taagtaattg actaatcgag tacctatttg
      421 gttctttcgt cgggagcttt ctacaactaa tggatgcaat tcccaacctg tgataccgta
      481 ttgagggatt gcaccaataa atcctactaa ctcatcttgg tctaccgctg ctaccgcgat
      541 tcgttctgga ttcatcattt cttctacttc ttctgccgag ctgtctccat attcttccgg
      601 ccaagtcagt ctcagtaaat cagaaagctg atctttcaat actggattat tacggtcaaa
      661 ttcactgatt atcatacgtc ttcccccttg ttcttgctta ttctaacagt ccttttgaaa
      721 aaacgaaatg ttctcgctca aaaatcttta gctaccaatc agtatctcta ttctttcatt
      781 atttatctta cttataaaat aaaaaaatta tacagaaaca ctttattcaa aataggaaag
      841 atcaggaaag ccatcaattg gaggaaaaaa ctctgtttgt tcatcatgat atagattatc
      901 ttttgcttta aaaaaaacca at
//
LOCUS       DAZKLD010000285          895 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.293, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000285 DAZKLD010000000
VERSION     DAZKLD010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 895)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 895)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..895
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.293"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            253..876
                     /locus_tag="ACG8KR_002993"
     CDS             253..876
                     /locus_tag="ACG8KR_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFQ0202265.1"
                     /translation="MYLAYLRVSSDDQSLARQHKLIENWSVKNNIAEDDLKIFEEKVS
                     GKNIENRPELQELISFIRERDTIIIPSLDRLARNSKDTKDLLQLFRSKGASIEILDLP
                     SFNGVTDPALRDLLTNLVIEVFSYVAENERKKIKERQKQGIEIAKKNGRYTGKKIQYH
                     ANATGKNKLIYDEIVDGLKKKETISKIAKNAGVTRKTVYRVKRDLGM"
BASE COUNT          330 a          129 c          167 g          269 t
ORIGIN      
        1 aaactaatgt ccttattttt gtgttcacat ttctgttacc taagaaatac ttaaaggagg
       61 tgatgtagcc agcaacgact acaacttgaa cccgctatct acagtttatt tgttgtttct
      121 tttgggtata ctctaatggt acaaatccat atttgcttaa agcgttgatt tttcagcggt
      181 ttttctttat tgcaaagtgt accctaagac ctgtatacta atagtaaata gattggatag
      241 gaatgatggc aaatgtattt agcttattta agagtttctt ccgacgatca aagtttagct
      301 cggcaacata agctaattga aaattggtca gtcaaaaata atattgctga agacgattta
      361 aaaattttcg aggaaaaagt ctctggaaaa aatattgaaa acagaccaga gctgcaagag
      421 cttatttctt tcatacgtga acgagatacg attattattc ctagtcttga tcgattagca
      481 agaaatagta aagatacaaa agacttactt caattgtttc gtagtaaagg tgcttctatt
      541 gaaatattag acctaccgtc gtttaatggg gtaactgacc ctgcattaag agacttgcta
      601 acaaatttag tcatagaagt gtttagctat gttgctgaaa acgaacggaa aaaaatcaaa
      661 gagcgtcaaa aacaaggcat tgaaattgca aagaaaaatg gtcgttatac tggtaaaaag
      721 attcaatacc atgccaatgc aacaggaaaa aacaaattga tttacgatga aattgttgat
      781 ggtctgaaga aaaaagaaac tatttctaaa atagcaaaga acgcaggagt tacaagaaaa
      841 accgtttatc gagtaaaaag agatttagga atgtaatatg ttccagttag aaaga
//
LOCUS       DAZKLD010000286          878 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.294, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000286 DAZKLD010000000
VERSION     DAZKLD010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 878)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 878)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..878
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.294"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..679)
                     /locus_tag="ACG8KR_002994"
     CDS             complement(<1..679)
                     /locus_tag="ACG8KR_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element IS1476 family transposase"
                     /protein_id="HFQ0202266.1"
                     /translation="MDNHTRKLLGLTDKHLFFDEEWLIEKEYKGVQAQFIKGKLDDSP
                     SYCEHCRSVRIIRNGSYTTRTQILKVKEKLTILELKRTRFLCHDCGRTFSAKTDLVDE
                     HHQLTKELKQAILMELYENQSRKLIAKKYFVSDGTVTRILREATKHYQSRMNFLPTVL
                     CMDEFKSMKSVSGSMSFICVDGTTHQLFTILEDRRLYKLTQYFLHFPRKARLKVKYLV
                     MDMNASYC"
BASE COUNT          263 a          172 c          164 g          279 t
ORIGIN      
        1 gacaatagct ggcattcatg tccatcacta ggtacttgac ctttaaacgg gcttttctag
       61 gaaagtgcag gaagtattga gttagtttgt aaagtctgcg atcttcaagg atcgtaaata
      121 gctgatgggt tgtcccatca acacaaataa aactcatgga accagaaaca gacttcattg
      181 atttgaactc atccatacac aacaccgtcg gaaggaagtt catacgtgac tggtaatgct
      241 tcgttgcttc acgcaagatc cgtgtcaccg tcccatccga gacgaagtac ttcttagcga
      301 ttagtttacg tgattgattt tcgtacagtt ccatgagaat tgcctgcttc agttcctttg
      361 ttagttgatg atgttcatca actaagtccg ttttcgcaga aaaggtcctt ccacaatcat
      421 ggcagagaaa acgggtacgt tttaattcta aaatagttaa cttttctttg actttcaata
      481 tctgagtgcg tgtcgtatag gaaccgttgc ggataatacg aaccgatcga cagtgttcac
      541 aataggaagg agagtcatcc aattttcctt taataaactg cgcttgaacg cctttatatt
      601 ctttttcaat cagccattct tcatcaaaaa ataaatgttt atctgttaat ccgagtaatt
      661 ttctagtatg attatccatt gagagactcc tttggtagga tgacgttttg gtcgactaaa
      721 ttttaccaaa agtgagtctc tttttgagtt gaaacgaaga aaaagtgtta gtagaaaatc
      781 gccttcgatt ttccaccaac acaaaatatt atagaagcaa aatcaattct accttagatg
      841 ttcttatatc actctatcag tcctatttga caaagagc
//
LOCUS       DAZKLD010000287          870 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.295, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000287 DAZKLD010000000
VERSION     DAZKLD010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 870)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 870)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..870
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.295"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>870
                     /locus_tag="ACG8KR_002995"
     CDS             <1..>870
                     /locus_tag="ACG8KR_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4041 domain-containing protein"
                     /protein_id="HFQ0202267.1"
                     /translation="EINQKYDETLKNVYSSLIVSNIEETSSSEIKNKLSLLKLKEKEY
                     IIDDAVSVPLILNKREKNNLKKKMLIPFDSEVKGLLNKLTISNIDSTREKIIKIFDKI
                     NNLFKNDNAELRKGLLEFKLQELELHYSYLVKITEEKEQQKAIKEQMIEEEKVRREID
                     REKKRIDKEQRQFNSEISKLIAYMQKANADVEKELYANKIQELEEKLKELEVIRENVL
                     QRELNTRAGYVYVISNIGSFGEDIYKIGMTRRLEPMDRIKELSSASVPFEFDVHAMIF
                     SEDAPSLETKLHN"
BASE COUNT          374 a           97 c          142 g          257 t
ORIGIN      
        1 atgaaataaa tcaaaagtat gatgaaacat taaaaaatgt ctattcttca cttatcgttt
       61 caaatataga agaaacttca tcttctgaaa taaaaaataa gctttctttg ttaaaattaa
      121 aagaaaagga atatattatt gacgatgctg tttctgtccc tcttatttta aataaacgag
      181 agaaaaataa tttaaagaaa aaaatgctca ttccatttga ttcagaagtg aaaggattac
      241 tcaataaact aactatttct aatatagact cgactaggga aaaaatcata aaaatatttg
      301 ataaaattaa taatttattc aagaatgaca atgctgaatt aaggaagggt cttttagagt
      361 ttaaacttca agaactagaa cttcattact cttatttagt gaaaattacc gaagaaaaag
      421 aacaacaaaa agcaataaaa gaacagatga tagaagagga aaaagttcgt agagaaattg
      481 atcgtgaaaa gaaacgtatt gataaagaac aacgtcaatt caattctgaa atttccaaat
      541 taatcgcata tatgcaaaaa gctaatgcag atgtggaaaa agaattatat gcaaataaaa
      601 ttcaagaact ggaagaaaaa ttaaaagaat tagaagttat acgtgaaaat gtattacaaa
      661 gagaattaaa tactagagcc ggttatgtgt atgtaattag taatattggg tcttttggtg
      721 aagacatata taaaattggt atgactagaa gattagaacc tatggataga ataaaagaat
      781 tgagtagtgc atcggttcca tttgaatttg acgttcatgc gatgattttt tcagaggacg
      841 ctccgtcttt ggaaactaaa ttgcataatc
//
LOCUS       DAZKLD010000288          846 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.296, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000288 DAZKLD010000000
VERSION     DAZKLD010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 846)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 846)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..846
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.296"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            68..643
                     /locus_tag="ACG8KR_002996"
     CDS             68..643
                     /locus_tag="ACG8KR_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytS/YhcK type 5TM receptor domain-containing
                     protein"
                     /protein_id="HFQ0202268.1"
                     /translation="MNKNKMATRVLTVMALCIGINYIGGTIALWLRLPIYLDSIGTIF
                     AGALLGPVPGMLTGLSSGILSGVTTDIFSLYYSPIQILTGLLAGLILPRYLGKDKNSL
                     KLLLLALILSVPGTIVSSIITVQLFGGITSSGSSMIVQLLHGLGLSQTTSVILVQAGT
                     DYLDRLLSIVVVSLVITMLPQRLIYSKRKIG"
     gene            complement(712..>846)
                     /locus_tag="ACG8KR_002997"
     CDS             complement(712..>846)
                     /locus_tag="ACG8KR_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFQ0202269.1"
                     /translation="FNQVNQGFISSVASKRNHIPRKSLNYQTPLEVFLSYVNGKFCLA
                     "
BASE COUNT          236 a          143 c          181 g          286 t
ORIGIN      
        1 gttcttttaa atgcacaaga agaaacgatt atgaaagatt tggaaaggtt aaagatgggc
       61 taacaaaatg aataaaaaca aaatggcaac tcgcgtactt acagtgatgg ctttgtgtat
      121 tgggatcaat tatattggag gaacgattgc tttatggctt cgtttgccaa tctatctcga
      181 ttctatcgga acgatttttg ctggtgcatt gcttgggcct gtacctggaa tgctgacagg
      241 attgtccagc ggtattttaa gtggagtgac tacagatatt ttttctctgt attattcgcc
      301 aatccaaatt ctcacaggct tgcttgctgg attgattttg ccaagatatc tcggcaaaga
      361 taaaaacagc ttgaaactgt tattactcgc tttgattttg tcggtacctg gaacgatcgt
      421 ttcttcgatc attaccgttc agttatttgg cgggatcacc tcctcaggat ccagtatgat
      481 cgttcagcta ttacatgggt taggtttaag tcagacaaca agtgtgatct tggtacaggc
      541 gggtacagac taccttgatc gcttgttgtc tatcgtcgtt gtttcattag tcatcactat
      601 gttgccccaa cgcttgatat attcaaaaag aaaaataggc tgaataaaca gagctggatt
      661 agcaaaataa tgctaatctg gctctgtttt ttcattttat tatttgtcaa attaagcgag
      721 acaaaacttt ccatttacgt aactcaaaaa aacttctaat ggtgtttggt aatttagtga
      781 ttttctaggg atatggtttc ttttggacgc aacggatgag atgaatcctt gattgacttg
      841 gttgaa
//
LOCUS       DAZKLD010000289          845 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.297, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000289 DAZKLD010000000
VERSION     DAZKLD010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 845)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 845)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..845
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.297"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..660
                     /locus_tag="ACG8KR_002998"
     CDS             <1..660
                     /locus_tag="ACG8KR_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease TnpB family protein"
                     /protein_id="HFQ0202270.1"
                     /translation="YISLLCKEEVRELPKSNSAVGIDLGIIDFAILSTGQKFDNNKFT
                     SKMEKKLKREQRKLSRRALLAKQKGINLFEARNYQTQKRKVARLHEKVMNQRTDFLNK
                     LSTEIIKNHDIICIEDLNTKGMLRNHKLAKSISDVSWSSFVSKLQYKADWYGRKIIKV
                     DKWFPSSQICSNCGHKDGKKSLEIREWTCPICHTHHDRDINASINILTEGLRLHSMGL
                     A"
     gene            736..>845
                     /locus_tag="ACG8KR_002999"
     CDS             736..>845
                     /locus_tag="ACG8KR_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFQ0202271.1"
                     /translation="MSKRTRRTFSQEFKQQIVNLYLAGKPRVEIIREYELT"
BASE COUNT          312 a          140 c          155 g          238 t
ORIGIN      
        1 tatatttctt tgttatgcaa agaagaagtt cgcgaactac ctaaatctaa ttctgctgtt
       61 ggtattgacc taggtattat agactttgca attctttcta ccggtcaaaa atttgataat
      121 aataaattta cgtccaaaat ggaaaagaaa ctcaaacgtg aacaacgtaa gttgtcaaga
      181 cgtgccttat tagctaaaca aaaaggtatc aatctgtttg aagctagaaa ttatcaaaca
      241 caaaaacgta aagtagcacg attgcatgaa aaagtaatga atcaacgtac tgattttctg
      301 aataagttga gtacagaaat tatcaaaaat cacgatatca tctgtattga agacttaaac
      361 acaaaaggca tgttgcgtaa tcataaacta gcaaaaagta tttcggatgt ttcttggtct
      421 agttttgtgt ctaaattaca atacaaggct gactggtacg gtcggaaaat catcaaagta
      481 gataagtggt ttccatctag ccaaatctgt tcgaattgtg gacataaaga cggcaagaaa
      541 tctctcgaaa ttcgagaatg gacttgccct atttgtcata ctcatcatga ccgagatatc
      601 aacgctagta tcaatatcct gaccgaaggt ctaaggctac actctatggg attggcttag
      661 tggataggct acaattaact agacagaaaa attaaggtgt gtagactaga agaaaacata
      721 ccaggaggaa tttttatgtc taagagaaca cgaagaactt tttcacaaga attcaagcaa
      781 caaatcgtca atctttactt agctggaaag ccacgtgtag aaatcattcg agaatatgaa
      841 ctaac
//
LOCUS       DAZKLD010000290          810 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.298, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000290 DAZKLD010000000
VERSION     DAZKLD010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 810)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 810)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..810
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.298"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          281 a           97 c          172 g          260 t
ORIGIN      
        1 tattatagaa aaaatttata aaaggaggtt tcttgatatg ataagaaaca aaaagggttt
       61 gttgaaagtt gctttactgg cagctttttt ctaagtaact ttttgacaag ctcaatggca
      121 acacaagcgg aagaagtaat acaagaacta actgttatga ataacagcta gataaaatgg
      181 gctatgtatg ttatccaaca atttagtaat cactagaagc ctagcgaaag ttcgcttgat
      241 aagttataga tgagtattga tttgtagtgt ttccaaatca aaagggtaca gagccacatg
      301 aaatatgttg tcctctgcag cgtcaggatg ggaatccata ccatggagat tcggagatct
      361 gtgagggaat acttacgtgg ttaatatgtc agttgtgttg gacgtagtaa tggtcatagt
      421 gggtagattg actgaattct tatatgagaa aatgaaacca acaagaagat agggttattt
      481 atacagaatt ggggatcttt tctggaaaag taatcggata atgcgagatt cgtacggtct
      541 tttttaaatt tctctaaaaa aaacggcatc gaatgggttg ggtgattgat aagcaatgaa
      601 tcatagactg atatagaata aaaaatgatt ctatttatct ttttacatct atcgtttttt
      661 caagtagccg caaattatat cagcattcaa caatttttgt atacaagttc tagtaaaagg
      721 atctgaagaa aaagttggtt gttgtgaaga taaaaaaaga atcaggtttt actgagtttt
      781 tcttcaaaat ttgtgttatt aggttaggac
//
LOCUS       DAZKLD010000291          796 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.299, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000291 DAZKLD010000000
VERSION     DAZKLD010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 796)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 796)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..796
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.299"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            45..737
                     /locus_tag="ACG8KR_003000"
     CDS             45..737
                     /locus_tag="ACG8KR_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFQ0202272.1"
                     /translation="MRKKIIINAGVIIGLMLVIFSGKQYADNILLWGGEDNIEKINNN
                     LTILDTALENKEQKISILNSQLSSNNQTLEQLRTNIEVSKQKVASLENDKNQLVSEKT
                     NLENQLSAKDSELQGKQNEINAKIDEINQKVAEINRLNSDWNNRLQNEKNKFNEEQST
                     INSLNQQINQMLSEKNNIQNQLQATQQKLEEEKSKNSSLNNYIEKLQKAKNDVEDTAN
                     KAQQIVNEHTIQ"
BASE COUNT          372 a           89 c          123 g          212 t
ORIGIN      
        1 tttaataact tatttaagga aattttttga aggagaagat aaaagtgaga aaaaagatta
       61 ttattaatgc tggagtaatt ataggattga tgttagtaat tttctccgga aagcaatatg
      121 ctgataatat tttattatgg ggaggggaag ataatattga aaagatcaac aacaatttaa
      181 ctatattaga cacagctcta gaaaataaag agcaaaaaat tagtatttta aattcacaat
      241 tgtcttcaaa caaccaaact cttgaacaac taagaactaa tattgaagtt tccaaacaaa
      301 aagtggcatc tttagaaaac gacaagaatc aattagtatc agagaaaact aatttagaaa
      361 atcaattgtc agcaaaagac tctgaattgc aaggaaaaca aaatgaaatt aatgcgaaaa
      421 tagacgaaat taaccaaaaa gtagctgaaa taaatcgatt aaatagcgat tggaataatc
      481 gtttacaaaa tgaaaagaac aaatttaatg aagaacaatc tactattaat agtttaaacc
      541 agcaaattaa tcaaatgttg agtgaaaaaa ataacattca aaatcaactg caagctactc
      601 agcaaaagct agaagaagaa aagtcaaaaa atagtagttt gaataattac attgaaaagc
      661 ttcaaaaagc taaaaatgat gtagaggata ccgcaaataa agcacaacaa attgtaaatg
      721 aacatacaat ccaatagata tagttttaat ttaacaaatg gtatgtttgg agataatgag
      781 atagaatata aatgtc
//
LOCUS       DAZKLD010000292          793 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.300, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000292 DAZKLD010000000
VERSION     DAZKLD010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 793)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 793)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..793
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.300"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..782
                     /locus_tag="ACG8KR_003001"
     CDS             <1..782
                     /locus_tag="ACG8KR_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFQ0202273.1"
                     /translation="DMLDYYYFEKNLSYNYVIHLKTFTGMIFKFANRRYGLKYNPVNG
                     VSLARKPKTNEEIKKEKDGYLSKEEVQMIASKQKSSHQQSRYSLITKFLFLTGLRYGE
                     LISLTESDYDGNKITVSGTYDYELKIKTTTKNTGSYRTIELSSNAKEIIDQLIEENKL
                     IKNNKDKYIFISKNGNPISIQAYNQSLRAVSKELNIDKKVSSHMLRHSHISLLTELGI
                     PLKAIMDRVGHEDSKTTLKIYTHTTKNMQNQLVEKLGKIEI"
BASE COUNT          340 a          107 c          102 g          244 t
ORIGIN      
        1 aagatatgct agattactac tattttgaaa aaaatttatc atataattat gtaatacatt
       61 taaaaacttt tacaggaatg atttttaaat tcgccaaccg tcgatatgga ttaaaatata
      121 atcccgtaaa tggggtttct ttagcaagaa aacctaagac aaacgaagaa ataaaaaaag
      181 aaaaagatgg ttatttatca aaagaagaag ttcaaatgat tgctagtaaa caaaaaagtt
      241 cacatcaaca gtcacgttat tctttaatta ctaaattttt gtttttaact ggactacgtt
      301 atggtgaatt gatatctttg actgaatctg attatgatgg taataaaatt acggtttctg
      361 gtacttatga ttacgagtta aaaattaaaa caacaactaa aaatacaggt tcttatagaa
      421 ccattgaatt atcaagtaat gctaaagaaa tcatagatca attaattgaa gaaaacaaat
      481 taattaagaa taataaagat aaatacattt tcatctctaa aaatggtaac ccgatatcta
      541 ttcaagcata taatcaatct ttaagagcag tatccaaaga attaaacata gataaaaaag
      601 tttcttctca catgttaagg cattcccaca tttctctact tacagaatta ggaatcccat
      661 taaaagccat tatggatcgt gtaggacatg aagattcaaa aacaacatta aagatttata
      721 ctcatacaac taaaaatatg caaaatcaac tagtagaaaa actaggaaaa atagaaattt
      781 aaattgcccc ttt
//
LOCUS       DAZKLD010000293          790 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.301, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000293 DAZKLD010000000
VERSION     DAZKLD010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..790
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.301"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(248..>790)
                     /locus_tag="ACG8KR_003002"
     CDS             complement(248..>790)
                     /locus_tag="ACG8KR_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
                     /protein_id="HFQ0202274.1"
                     /translation="KEKSIVIESMKDGLHIYDNPVGVLTNNPSFDYQLFNLNNYRVLS
                     SETPKNNFSNQISLNAYSRGMGGIGLPGDLSSVSRFVKATFTKLNSVSGDSESESISQ
                     FFHILGSVEQQKGLCDVGDGKYEYTIYSSCCNVDKGIYYYRTYEDSQITAIDMNKEDL
                     DSHKLISYPIIEKQQIKYIN"
BASE COUNT          292 a          129 c          100 g          269 t
ORIGIN      
        1 catgcttaaa aggtctatgg attaagttat gtaaaaaagt gcctctttaa acgtcctaaa
       61 atccaaacaa ggatagcata atatacacta tcgttgatcc agaaaacagg taaataaatt
      121 ttgcaaaagc aataagttaa aaacattaca aaaaagacaa ggatatcaca tcaagaaaaa
      181 atgttttgaa aatatctttg cctttttgta ttatatctat attaaataat caatcacaac
      241 acattaacta atttatatat ttaatttgtt gtttttctat aattggataa ctaattaact
      301 tatgactatc taagtcttct ttattcatat caattgcagt aatttgactg tcttcatatg
      361 ttcgataata atagattcct ttgtcaacat tgcaacaaga agaataaatt gtatattcat
      421 attttccatc accaacatca cacaaacctt tttgttgttc tactgaacct aagatatgga
      481 aaaattgact aatactttct gactctgaat ctccagatac agaattcagc ttcgtaaaag
      541 tcgctttaac aaaacgagat actgaggata aatctccagg caagcctatc cctcccatac
      601 cgcggctata ggcattcaaa cttatttgat ttgaaaaatt atttttagga gtttcactcg
      661 ataagacacg ataattgttt aaattaaata attgatagtc aaatgaagga ttattggtaa
      721 gaacgcccac agggttatca tatatatgaa gtccatcttt catactttca atgacaattg
      781 atttttcttt
//
LOCUS       DAZKLD010000294          786 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.302, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000294 DAZKLD010000000
VERSION     DAZKLD010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 786)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 786)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..786
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.302"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>786
                     /locus_tag="ACG8KR_003003"
     rRNA            <1..>786
                     /locus_tag="ACG8KR_003003"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          223 a          172 c          232 g          159 t
ORIGIN      
        1 gaaataatag taccgaagtt cctgatgtca cactgccgag aaaagcttct agtgagaaaa
       61 cagcggcccg taccgcaaac cgacacaggt agtcgaggag agaatcctaa ggtgagcgag
      121 agaactctcg ttaaggaact cggcaaaatg accccgtaac ttcgggagaa ggggtgctga
      181 tcatacgatc agccgcagtg aataggccca agcgactgtt tatcaaaaac acaggtctct
      241 gcaaaatcgt aagatgaagt ataggggctg acgcctgccc ggtgctggaa ggttaagagg
      301 agtgcttagc gcaagcgaag gtacgaattg aagccccagt aaacggcggc cgtaactata
      361 acggtcctaa ggtagcgaaa ttccttgtcg ggtaagttcc gacccgcacg aaaggcgtaa
      421 cgatttgggc actgtctcaa cgagagactc ggtgaaattt tagtacctgt gaagatgcag
      481 gttacccgcg acaggacgga aagaccccat ggagctttac tgtagtttga tattgagtgt
      541 ctgtaccgca tgtacaggat aggtaggagc cgtagaaatc ggaacgctag tttcgatgga
      601 ggcgctggtg ggatactacc cctgcgttat ggccactcta acccgcacca ctaatcgtgg
      661 tgggagacag tgtcagatgg gcagtttgac tggggcggtc gcctcctaaa aggtaacgga
      721 ggcgcccaaa ggttccctca gaatggttgg aaatcattcg aagagtgtaa aggcagaagg
      781 gagctt
//
LOCUS       DAZKLD010000295          758 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.303, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000295 DAZKLD010000000
VERSION     DAZKLD010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 758)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 758)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..758
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.303"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(504..728)
                     /locus_tag="ACG8KR_003004"
     CDS             complement(504..728)
                     /locus_tag="ACG8KR_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0202275.1"
                     /translation="MKSDKYKNRKHTSWKTFREIIGSYLAVVVFLPLLYMAMTEQTRF
                     IGVDTQYIAETGFLFIFILIFAFYKSFHTK"
BASE COUNT          267 a          109 c          133 g          249 t
ORIGIN      
        1 ctctatttac ttctttttta atttgttctt gcaatctttt ttgctacgta atttttgtaa
       61 aaaagtgggt tgagagaaat ctaattgatt ttcaaagaag gagtatgaaa atgagaaaaa
      121 tatttattcg tttattgtgg gtactatctt agaggaataa ataatagtgg agaaaactat
      181 ggtttggcgc agattctatc aggtttgatg gaacgaagac aacctcccga catcaagtca
      241 aaagctactt gagattttga tctgaaaata aaaaaagaaa acaaggtagg aacatcaatg
      301 tttctacctt gttttcgtaa tcaatgggaa aaaactaaga gtggtgtacg taatcaataa
      361 aaaggatggt gacaacagta cctctttact ctctgaaagc atcataactc gtttttcctc
      421 attatgccaa taaattgttg ttataccaca tataaggatt tgtaatagct aaaaacagac
      481 aggaaagttg aaatctgtga atcttatttt gtatggaagg atttatagaa cgcaaaaatc
      541 aagataaaga tgaataaaaa tcctgtttcg gctatatatt gcgtatctac gccaataaaa
      601 cgagtttgtt ctgtcatagc catataaagc aaaggcaaaa atacaactac agcgagataa
      661 gatccgatga tttctcgaaa agttttccag gaagtatgtt tcctgttttt atatttatcc
      721 gatttcattg aatagtcata gcctccttat tctttcat
//
LOCUS       DAZKLD010000296          757 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.304, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000296 DAZKLD010000000
VERSION     DAZKLD010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 757)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 757)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..757
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.304"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>757)
                     /locus_tag="ACG8KR_003005"
     CDS             complement(<1..>757)
                     /locus_tag="ACG8KR_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="O-antigen ligase domain-containing protein"
                     /protein_id="HFQ0202276.1"
                     /translation="IIRAINFYSLLPSTFDSIFFKLIGISGVLLLGIDFIIKFNKRKV
                     TYNILLIAYMVILVITSFVHKEYGLGENLKTIMWAILQYFLIYQFAFENSDNKRFFKR
                     ISWGFIYSWFVLVSMSMVLFFGKFGYEKYYNARHRIRIGFLESRLFGTFSDINNAAIA
                     SLVVIVLILFYLYNKKVTGLNKIIFLINLPLQFIYIVLSGSRSTYVVSIIIVAISSFI
                     VVLKRNGTKKDLKNYILAIVSSIGSVLILFALF"
BASE COUNT          315 a          121 c           75 g          246 t
ORIGIN      
        1 ttaaacaaag cgaataaaat cagaacagaa ccaatagaag atactatagc aaggatatag
       61 ttttttaaat ctttttttgt tccatttcgt tttaacacaa caataaaaga tgatatggca
      121 actattatta tacttaccac ataggtagat cgtgacccac ttaatactat ataaataaat
      181 tgtaatggaa gattaattaa aaaaatgatt ttatttaatc ctgtcacttt tttattataa
      241 agataaaata agattaggac aataacaact aaagatgcta tagcagcatt gttaatatca
      301 gaaaacgtcc caaacagtct tgattctaaa aaaccaattc tgatcctatg tctagcatta
      361 taatatttct catagccgaa tttaccaaag aaaagaacca ttgacattga tactaaaaca
      421 aaccaactat aaataaatcc ccaacttatc cttttaaaaa atcgtttatt atcgctattc
      481 tcaaaagcaa actgataaat caaaaaatac tgtaagattg cccacattat agtctttaag
      541 ttttctccta gaccatattc tttatgaaca aaactcgtta ttactaaaat caccatatat
      601 gctatcaata aaatgttata agttactttt cttttattaa atttaattat gaaatctatc
      661 cctaatagca aaactcctga aattcctata agtttaaaaa aaatactatc aaaagtggac
      721 ggtaataaac tataaaaatt tattgcacga ataatca
//
LOCUS       DAZKLD010000297          747 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.305, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000297 DAZKLD010000000
VERSION     DAZKLD010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 747)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 747)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..747
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.305"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..672
                     /locus_tag="ACG8KR_003006"
     CDS             <1..672
                     /locus_tag="ACG8KR_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease TnpB family protein"
                     /protein_id="HFQ0202277.1"
                     /translation="SGKYYISLLCKEEIIELPKTNSTIGIDLGIMDFAILSDGQKIDN
                     NKFTSKMEKKLKREQRKLSRRALLAKNKGTNLFEAKNYQKQKRKVARLHEKVMNQRTD
                     FLNKLSTEMIKNHDIICIEDLNTKGMLRNHKLAKSISDVSWSKFVTKLQYKADWYGRK
                     IIKVDKWFPSSQICSECGHKDGKKSLEIREWTCPVCHTHHDRDINASINILTEGLRLH
                     SMGLA"
BASE COUNT          280 a          108 c          141 g          218 t
ORIGIN      
        1 agcggtaaat actatatttc tttgttgtgt aaagaagaaa ttatcgaatt acctaaaact
       61 aattccacaa ttggtattga cttaggtatt atggactttg cgattctttc tgatggacaa
      121 aaaattgata ataataaatt cacgtccaaa atggaaaaga aactaaagcg tgaacaacgc
      181 aaattgtcta ggcgtgcttt attggctaaa aataaaggta ccaatctatt tgaagctaaa
      241 aattatcaaa aacaaaaacg caaagtagct agattgcatg aaaaagtaat gaatcaacgt
      301 actgattttc tgaataagtt gagtacagaa atgatcaaaa atcacgatat tatctgtatt
      361 gaagacttaa acacaaaagg tatgttgcga aatcataaat tagctaaaag tatttctgat
      421 gtttcttggt caaagtttgt gactaaatta caatacaagg ctgactggta tggacgaaaa
      481 atcatcaaag tagataaatg gtttccgtct agtcagattt gttcagagtg cggacataaa
      541 gatggcaaga aatctcttga aattcgagaa tggacttgtc ctgtttgtca tactcatcat
      601 gatcgtgata tcaatgctag tatcaatatt ctgaccgaag gtctaagact acattctatg
      661 ggattggctt agatccaatc atcaagaacc gtaggaacta cggggatagc ttggtaaata
      721 agagacaccg ctgttagtaa agaaata
//
LOCUS       DAZKLD010000298          736 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.306, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000298 DAZKLD010000000
VERSION     DAZKLD010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..736
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.306"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          247 a          128 c           95 g          266 t
ORIGIN      
        1 gtatgtccta tgatgaaaag attgtactat tcattgatca taacgattgg ctatttgatt
       61 gtatcaaatc taggaaatat ggtatttggt atcagtaaag aattcagttg gactactaca
      121 ttatgggaat ctctattctt tttcatcttc gtatttttgt tacaaaatta tcgaaaaaaa
      181 taaacatgtg ccatctatct gttggcacat gttttaggat ttatggtact ttttattcgg
      241 caatccattt tatttttcta cttcttctta atacctctaa cacttttgat ttataaacta
      301 atccaatgca tctacttgcg cttattaagc tatttcagct agatagtacc accccatgcg
      361 tcaatgcttg attttccttt tgtataacat tgtataagca ataaaggaca aataagtggt
      421 gattacacca aaccattgaa taagaaaaat tctgatacgt aaataaacat gaacttgaaa
      481 aaattaatga atcggttagc tactacgtag ttgtcttttt tctcatacag ttctgttaac
      541 ttacacttct cttcgctaga taataaaaaa tcctccaact gtattgtcca atataatccc
      601 aggcatctaa actagcatta tagaagagac acacatagaa tctaaaataa ctggttttaa
      661 atgtttctta caatcaaaaa aagctgttaa atgcactcca caccttcata ctctatcatt
      721 ttttataaac gatata
//
LOCUS       DAZKLD010000299          725 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.307, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000299 DAZKLD010000000
VERSION     DAZKLD010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 725)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 725)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..725
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.307"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>725
                     /locus_tag="ACG8KR_003007"
     CDS             <1..>725
                     /locus_tag="ACG8KR_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0202278.1"
                     /translation="YRFTAESREEINKSLGENQQITYTKGFFLLTFIATHPELQHDFS
                     DETIQNLQISINDFKPKQQVLTEIEYYYRLRSLAVLGNNNEELQNHLNSLVDPILQQK
                     MRVSSLPSSTLETIYNQYLYFGEKEMFIDFMIEHLTDASSFRLADKDMEVLPWIYNKL
                     NQEQFNKLLYNLNGNNQFYKNSNFPPFISNLLDFYNQKFQVNLRKHYSGLLYPNAVTM
                     DQGFQLKDKELKKLYELAESKSD"
BASE COUNT          287 a          111 c          103 g          224 t
ORIGIN      
        1 gtataggttc acagctgaat caagagagga aattaataaa tcacttggtg aaaatcaaca
       61 gatcacgtat acaaaagggt tctttttact tacttttata gctacccatc cagaattaca
      121 acatgatttt tcagacgaga caattcaaaa tttacaaata tcaataaatg attttaaacc
      181 gaaacaacaa gtactaacag aaatagaata ttactaccgt ttacgttccc ttgcagttct
      241 aggaaacaac aatgaagaat tacaaaatca cttaaatagt ctagttgatc ctatactcca
      301 gcaaaaaatg agagtttctt cgctaccatc ttcaacatta gaaacaatat ataatcaata
      361 tttatatttt ggtgaaaaag agatgttcat agattttatg atcgaacatc ttaccgatgc
      421 tagttcattc aggttagcag ataaggatat ggaagtatta ccatggattt acaataaatt
      481 aaatcaggag caatttaaca aattactata caacctaaat ggaaataatc aattttacaa
      541 aaacagtaat tttccgcctt ttatttctaa tctgttagac ttttataatc agaaattcca
      601 agtgaattta cgtaaacatt atagtgggct tctatatcca aatgcagtga ctatggatca
      661 gggttttcaa ttaaaggata aagaattaaa aaaactgtat gaattagctg aatcgaaaag
      721 tgata
//
LOCUS       DAZKLD010000300          685 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.309, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000300 DAZKLD010000000
VERSION     DAZKLD010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 685)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 685)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..685
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.309"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(69..359)
                     /locus_tag="ACG8KR_003008"
     CDS             complement(69..359)
                     /locus_tag="ACG8KR_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0202279.1"
                     /translation="MGEKKIKGAGILLVMSKALFLLPCTFMFIVLCYFKRRKKWCWID
                     VLLFLPFFPLIYFLEEIWDDMACDSAFLFSHSLGLMISLTIFAIVLFFLNKK"
     gene            complement(477..683)
                     /locus_tag="ACG8KR_003009"
     CDS             complement(477..683)
                     /locus_tag="ACG8KR_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0202280.1"
                     /translation="MIMTSAFFYLFKRPNKYLLLSACPMIYFIHKIRGFYYYDSGISL
                     FCYEIGLLASAIFFIFVLEYFRKK"
BASE COUNT          288 a          111 c          104 g          182 t
ORIGIN      
        1 agataacacc caactttcaa aaatatgttt tggaagttgg gacaaacgag ttaatacatt
       61 ttaatacatt attttttatt caaaaaaaat agcacaatgg caaagatcgt aagagagatc
      121 ataagcccca atgaatgact aaaaagaaaa gcagaatcac atgccatatc atcccatatt
      181 tcttcaagaa aataaataag cgggaaaaag ggaaggaata gtagaacatc gatccagcac
      241 catttttttc ttcttttgaa ataacataaa acaataaaca tgaacgtgca gggaagtaaa
      301 aacaaagctt tactcataac taacaaaata ccagctcctt ttattttttt ctcccccaca
      361 ttcaaagagt aacaatggta tgtacttaat aatagaaaaa tggatcaact tgtatgaata
      421 acagctgaaa agatctgcac caaataaaaa acttccagcc aaaactgact ggaagtttat
      481 ttttttctga aatattctaa cacaaatatg aaaaagatgg ccgaagctaa taagccgatt
      541 tcgtagcaaa acaatgagat accagagtcg taataataaa atcctctaat cttatggata
      601 aaatagatca tcggacaagc agataagagt aagtatttgt taggcctttt gaataagtaa
      661 aagaaagcac tggtcattat gataa
//
LOCUS       DAZKLD010000301          673 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.310, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000301 DAZKLD010000000
VERSION     DAZKLD010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 673)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 673)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..673
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.310"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>673
                     /locus_tag="ACG8KR_003010"
     CDS             <1..>673
                     /locus_tag="ACG8KR_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0202281.1"
                     /translation="DEIKKVKNPRIIPLLEEVRSSFYQGNYRSAIAVNYSALILDLID
                     KLSDLANIYEDNTSKKILEDIARLRKSDPNSPKWEMELLEKAYSSTELIDSYEYEDLK
                     YIKDQRNYSAHPVVTYNGNTWELRDITKETCQDVIRKSYEIIFLKNPILGKKVTIEII
                     QYASRIHAMSVTPEEFHSALKNSYLNKLSEKAKENLCKTAFKFVFQLSYGNEEADRDR
                     ESTFRL"
BASE COUNT          276 a           88 c          111 g          198 t
ORIGIN      
        1 tgatgaaata aaaaaagtta aaaatcctag aattattcca ttattagaag aagttagaag
       61 tagtttttat cagggaaatt atcgttctgc aatagctgtc aattacagtg cgcttatttt
      121 agatttaatt gataaattat ctgatttagc aaatatttac gaggataata cttctaagaa
      181 aattttagaa gatatagcta gactaagaaa aagtgatcca aactctccaa aatgggaaat
      241 ggaactacta gaaaaagctt atagttcaac tgagctaatt gatagctatg aatatgagga
      301 tttaaaatat attaaggatc aaagaaatta ttcagctcat cctgtagtca cttacaacgg
      361 taatacttgg gaattaagag atataacaaa agaaacttgt caggatgtta tacggaagtc
      421 ctatgaaatt atattcctaa aaaatccaat attaggaaaa aaagtaacga tcgaaattat
      481 acaatatgca agtcgcatac atgctatgtc tgttacacct gaagaatttc actctgcact
      541 aaaaaatagc tatctaaata aactaagcga aaaagccaaa gaaaacctat gtaagacagc
      601 ctttaagttt gtttttcaat tatcttatgg aaatgaggaa gcagatagag atagggaaag
      661 tactttcaga cta
//
LOCUS       DAZKLD010000302          657 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.311, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000302 DAZKLD010000000
VERSION     DAZKLD010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 657)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 657)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..657
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.311"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            211..>657
                     /locus_tag="ACG8KR_003011"
     CDS             211..>657
                     /locus_tag="ACG8KR_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA];
                     GO_process: GO:0043170 - macromolecule metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043412 - macromolecule modification
                     [Evidence IEA];
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IbrB-like domain-containing protein"
                     /protein_id="HFQ0202282.1"
                     /translation="MNKDITFPVMDVRLVPITKIQANDYNPNRVASPEMNLLELSIIE
                     DGFTQPLVCYYKEKEEKYILVDVLHRYRVAKERLNLKVVPVTVIDKPIEERMASTIRH
                     NRARGTHEIRGMEIVIQRLVKNGWSDKRISKELGMDIEEIFRFKQVS"
BASE COUNT          229 a          100 c          131 g          197 t
ORIGIN      
        1 agaattttta tggatttttt aggatacaaa ttaattattt ttgcttttat cctgattacc
       61 agtccagtac tgtcggtatc ccattttgca gcaaaatcat gttgataaag tgacaagaag
      121 actgatgaga tatattatcc aaattgagaa gataattaat cggattcttg agtataccct
      181 actttttaag aaagtagggt atacaagcgt atgaataaag acataacttt ccctgtgatg
      241 gacgttcggc ttgttccaat taccaaaata caggcgaacg attataatcc caatcgagta
      301 gcgtctccgg aaatgaactt attggagctt tctatcattg aagatggctt tactcaacca
      361 ttagtttgtt attacaagga aaaagaagag aagtatatct tggttgatgt gcttcatagg
      421 tatcgtgttg ctaaggaaag actcaactta aaagttgtac cggtaacagt tattgataaa
      481 cccatagaag aacgtatggc ttcaacaatt cgacacaatc gtgcaagagg gacccatgaa
      541 ataagaggaa tggaaattgt catccaaaga ttagtaaaaa atggatggtc agacaagaga
      601 atatccaaag aattaggcat ggacattgaa gaaatttttc gctttaagca agtgtct
//
LOCUS       DAZKLD010000303          631 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.312, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000303 DAZKLD010000000
VERSION     DAZKLD010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 631)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 631)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..631
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.312"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            3..359
                     /locus_tag="ACG8KR_003012"
     CDS             3..359
                     /locus_tag="ACG8KR_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFQ0202283.1"
                     /translation="MEKISFETFKRPNGHDEFLEWLETLPKKDSAKLLRTIEETEKNG
                     MLVAQRLKWVKKLDTNLYELRSKVGSNIQRAIYFHVDNGRYVITHGFTKKTEKTPITE
                     LKHAKELREEWFTNDD"
     gene            349..615
                     /locus_tag="ACG8KR_003013"
     CDS             349..615
                     /locus_tag="ACG8KR_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFQ0202284.1"
                     /translation="MMIKKLIEEKKKDKDFAEAYDREGRKLETAVALYHAREEAGLTQ
                     AELAQKAHTTQSTIARIERGDNVSFDKLDDIARALGKKLVVEFA"
BASE COUNT          254 a           89 c          139 g          149 t
ORIGIN      
        1 gtatggaaaa aatatctttt gaaacattta aaagacccaa tggtcatgat gaatttttag
       61 aatggttgga aacattacca aaaaaagata gtgcgaagct tttacgtaca atagaagaaa
      121 cagaaaagaa tgggatgcta gtagctcaac ggttgaaatg ggtgaaaaaa ttagacacta
      181 atttgtacga attaagaagt aaggtaggat caaacatcca aagagccatc tatttccatg
      241 tggacaatgg aaggtatgtc attactcacg gatttactaa aaaaacggag aaaacaccaa
      301 taactgaact caagcatgca aaagaactta gagaggagtg gttcacaaat gatgattaaa
      361 aaattgattg aagaaaagaa aaaagataaa gattttgcag aagcatatga ccgtgaagga
      421 agaaaactcg aaactgcggt tgctctttat catgctcggg aggaagcagg gctaactcaa
      481 gcggagcttg cacaaaaagc acatacaact caatctacta ttgctcgaat tgagagaggg
      541 gataacgtat ctttcgataa acttgatgat atcgccaggg cattaggtaa aaagctggta
      601 gtagaatttg cttaaaagcg agttgaaaat a
//
LOCUS       DAZKLD010000304          629 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.313, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000304 DAZKLD010000000
VERSION     DAZKLD010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 629)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 629)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..629
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.313"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>629
                     /locus_tag="ACG8KR_003014"
     CDS             <1..>629
                     /locus_tag="ACG8KR_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS30-like element IS6770 family transposase"
                     /protein_id="HFQ0202285.1"
                     /translation="LYRLFSKGIFDIDTLPMKGKRKPNGHQEKRGKQQYQRSIHDRPD
                     NYPDFNSEFGHLEGDTIVGIHHKSAVITLVERLSKVIITIKPNGRKALDIETALNQWF
                     SRFPKNFFKSITFDCGKEFSNWKAISNQHDIDIYFADPGTPSQRPLNENSNGILRRNG
                     LPKSMDFRKVNQTFISSVSNQRNHIPRKSLNYRTPIEIFLSYVQEAFYS"
BASE COUNT          214 a          121 c          105 g          189 t
ORIGIN      
        1 cactttatcg tttattttct aaaggaatat ttgatattga cacactaccg atgaaaggta
       61 aaagaaaacc caatggccat caggaaaaac ggggaaaaca acagtatcag cgctcaatcc
      121 atgatcgacc tgataattat cctgatttca attctgagtt tggtcacctt gaaggtgata
      181 cgatcgttgg cattcatcat aaaagtgccg tcattacttt agttgaaaga ttatctaaag
      241 tcattatcac gattaaaccc aacggccgta aggcattaga tattgaaact gcccttaatc
      301 aatggttttc tcgcttccct aaaaacttct ttaaatctat tacgtttgac tgtggaaaag
      361 aattttctaa ctggaaagcc attagtaacc aacatgatat tgatatatat tttgcggacc
      421 ctggaacacc ttctcaacgc ccattaaacg agaattctaa cgggattctg cgtcgtaatg
      481 gactgccgaa atcaatggat tttagaaaag tgaatcagac atttatttcc agtgtcagca
      541 atcaacgtaa tcatattcca agaaaatcat tgaattacag aacaccaatc gaaatatttt
      601 tgagctatgt acaagaagca ttttattct
//
LOCUS       DAZKLD010000305          595 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.314, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000305 DAZKLD010000000
VERSION     DAZKLD010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 595)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 595)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..595
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.314"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            132..344
                     /locus_tag="ACG8KR_003015"
     CDS             132..344
                     /locus_tag="ACG8KR_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014834536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0202286.1"
                     /translation="MAVWQIQLKERGAYGLISLSDVLCRKEMPDLFSLDQIIAIIGAA
                     AVVITAVAKASDSILAWLKFKKEQKK"
BASE COUNT          210 a           90 c          104 g          191 t
ORIGIN      
        1 aaaacactat atattccgaa aagtgagtat ctccattcat aaaataatca acatatttaa
       61 aaaatctaat tagctttttt agtcaatcta aagttcttta ttctatgata taatgttact
      121 gtagaaagtt tgtggcggtt tggcaaatcc aattgaagga gcgtggtgcc tatgggctta
      181 ttagcttatc ggacgtactc tgtagaaagg aaatgcctga tttgttctca ttggatcaaa
      241 tcattgctat tatcggagca gcagcagtag ttattactgc cgtagcgaaa gctagcgatt
      301 cgattttagc ttggttaaag ttcaaaaaag aacaaaaaaa atagagccgt ctcaactttt
      361 ggcgaggtag acgagctcta aagctttatt tacatgccat ccgctttgaa cggactacac
      421 tgggcatgtg acagcatgcc ctttcattta tattatatct cattctttta tataatgaaa
      481 gtaaataaat catggaggat aaaatatgga taaatttaat agattaacat taataaatca
      541 gtttgaaatt ttaaaagctt taaatcctaa cgaagaaaaa tactatgctg aaaaa
//
LOCUS       DAZKLD010000306          595 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.315, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000306 DAZKLD010000000
VERSION     DAZKLD010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 595)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 595)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..595
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.315"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            44..379
                     /locus_tag="ACG8KR_003016"
     CDS             44..379
                     /locus_tag="ACG8KR_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0202287.1"
                     /translation="MKQYLNEWKAIEGSLVTQRIKELPDCLEKDHLFQIREMLKKEQF
                     DPDQFLVVEYPTIGVYCCNHINDGKYFIIQEYGGQLTPFYTTWEMSEEGINNFPCESI
                     EDSISHTEC"
BASE COUNT          231 a           66 c          107 g          191 t
ORIGIN      
        1 aatttcctag gaaaaatagt taacataaaa ggaatgaatt gttatgaaac aatatttaaa
       61 tgaatggaaa gcgatagagg ggtcattagt aacgcaaaga ataaaggaat tacctgattg
      121 cttagagaag gatcatttgt ttcaaatacg agaaatgttg aaaaaagaac agtttgatcc
      181 agaccaattt ttagtagtcg agtatccaac tattggagtg tactgttgta accatatcaa
      241 tgatggaaaa tattttatta ttcaggagta tggaggacag ttaactcctt tctatactac
      301 atgggaaatg agtgaagaag gaatcaataa ttttccatgt gaaagcatcg aagattcaat
      361 ttcacacacc gaatgttaat tgattataac tataaaaaag gattgaacat tatgttcaat
      421 ccttttttca atgaaatgtt ttccgccatt tcattgtttg gtgcgatttc tattttatta
      481 tatcaaaata aagagagatt ctaaagaaag aagagggaaa tatataaatc aagcgacagt
      541 atatttatta ttaaatgcta ttgtgagaat ataactaatt agtttaattt gaaag
//
LOCUS       DAZKLD010000307          593 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.316, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000307 DAZKLD010000000
VERSION     DAZKLD010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..593
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.316"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            23..593
                     /locus_tag="ACG8KR_003017"
                     /pseudo
     CDS             23..593
                     /locus_tag="ACG8KR_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
BASE COUNT          205 a           96 c           88 g          204 t
ORIGIN      
        1 taagtaaaaa ggagcctctt tcatgcaaac tcaagaagcg attaaaacgt ttatcttgaa
       61 gaaaaaaagc aatacaaaat tagatatttt tcttttccta tctgaacatc gtttctttat
      121 tactacacaa tatttagcag atcatttcca tatgtctgaa tcaaattttt tactatacat
      181 caaagaatta gaacaagact ttgagcgtct caatctaaca gaactgcata ttgataagca
      241 aaaacctttt ttaaagttga attttgaagg aattgatccg gcttactgct attatcgtct
      301 atttggaaga tactgcaacg aatcagtcag ttatcagatc ttgacttctt tgttcagctg
      361 tcagacaaat tcgattatca gtttcagtca gcaaacaaac tatagtgcct cttatttgta
      421 tacgaaaatg aaaaagatta atgcattttt agcatagtac ggtttgtcta tctccttttc
      481 taataatggc agaaagtctt tttctggaaa agaggtacaa attcaatatg cagcattaga
      541 tatttattgg aacatctttt caagtactgc tacagaattt tatgatgaag atc
//
LOCUS       DAZKLD010000308          589 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.317, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000308 DAZKLD010000000
VERSION     DAZKLD010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 589)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 589)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..589
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.317"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>589
                     /locus_tag="ACG8KR_003018"
     CDS             <1..>589
                     /locus_tag="ACG8KR_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0202288.1"
                     /translation="DSRFFVSNLQDDELIRQIDSLLETITESDKRIFLNYVELTRHII
                     ELDKLFNVFRYNLTNLLKHFTIFTNDLIESTGEKLTEDQYYYQINALTINLISSAKTL
                     TESIEVCMKNFLAEKDFKSFKNKILSKPYDEHFSYRFLLHMRNYSQHGHLPVNIHQQR
                     VYFDLDEILAMPHFDFNGQLKKELDVLRKDIYNQFR"
BASE COUNT          224 a           65 c           93 g          207 t
ORIGIN      
        1 gatagtaggt tttttgtcag taatttacaa gatgacgagc taataaggca gattgattca
       61 ttattagaaa caataacaga atctgataaa agaatatttt taaattatgt agaattaaca
      121 cgtcatatta tagagttgga taaacttttt aatgtgttta gatataattt aactaatttg
      181 ctcaaacact tcacaatttt tactaatgat ttaattgaaa gtacaggtga aaaattaacg
      241 gaagaccagt attattatca gataaatgct ttgacgataa acttaattag ttcagccaaa
      301 actttgactg aatccattga agtttgtatg aaaaactttt tagcagaaaa agattttaaa
      361 tcttttaaga ataaaattct gagtaagcct tatgatgagc atttttcgta taggttttta
      421 ttacatatga gaaattattc tcagcatggt catctgccag taaatattca tcaacaaaga
      481 gtgtactttg atttggatga aattctagca atgcctcatt ttgattttaa cggacaatta
      541 aaaaaggagt tagatgtttt aaggaaagat atatataacc aatttagag
//
LOCUS       DAZKLD010000309          586 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.318, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000309 DAZKLD010000000
VERSION     DAZKLD010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 586)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 586)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..586
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.318"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          224 a           76 c           76 g          210 t
ORIGIN      
        1 attagcaata tataaaagaa ggtatcaaga gtcacagtga atttattccg aaaagtgaaa
       61 agagcaaata taaaatttaa taaaaataag aactaaatag gaaaacgtag acgtaatttt
      121 ctacgttttc cttttcatat atattaaata ttacggaaaa ttatataaac taatttagtg
      181 ttttgtttgt aattcctaat tttgctccac ttttagataa tttttaggtc tatttaactc
      241 attagacatt ctatgctcat ttttctccaa atttgtttca actctttcgt acttaatttt
      301 gcgtatttga aaattcttct tttagagtac ttttattacg atactagtat gatatatatc
      361 tacagcaaac agttaaaatc ttcctaaaat aagcgaatag cattcctaaa acctataaaa
      421 tttttagatg aagcgacaag tggacaatta ataaactact tgaacatgaa attatggatc
      481 acctttcaaa tttagaaaat ggaattctta tacttattgc gcaacattta ttgattgcaa
      541 aattatgtga caaaaagaac tagtagaaga aggaataatg gcttga
//
LOCUS       DAZKLD010000310          585 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.319, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000310 DAZKLD010000000
VERSION     DAZKLD010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 585)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 585)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..585
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.319"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>585)
                     /locus_tag="ACG8KR_003019"
     CDS             complement(<1..>585)
                     /locus_tag="ACG8KR_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0202289.1"
                     /translation="EIQRRTTNFLHLGSFFATLTILFFLCYSYLKTSHAPSSALGWLL
                     LDHSDITEAEENPFFLILSSLCGLLFSVYFGAYFFHRHPGTLNEASSVLIAFKTFSFL
                     AILFYSFNHNTLFFFIMNGLVVILSIFTYFIFSLIFSQLKCPLFFRLVPVTCFIYSLF
                     SFFVLALMPLTNPSTIQVAGNLLDMLFIVSLGVF"
BASE COUNT          259 a           87 c          116 g          123 t
ORIGIN      
        1 taaatacacc tagactgacg atgaagagca tatccaacag atttcctgcg acttggatcg
       61 tcgaagggtt ggtcagtggc attaaagcaa ggacaaaaaa ggaaaacaag ctgtaaataa
      121 aacaggtgac aggaactaat cgaaagaaga gcggacattt caattggcta aaaatgagtg
      181 aaaaaataaa ataagtaaaa atggataaaa tcacgactaa accattcatg ataaagaaga
      241 aaagcgtatt gtgattaaac gaataaaaaa gaattgctaa aaaagaaaac gttttaaatg
      301 caatcaatac gcttgaagct tcgtttaacg taccaggatg tcgatgaaaa aaataggcac
      361 cgaaataaac agaaaacagt aagccgcaaa gagaagacaa aatcaaaaaa aacggatttt
      421 cttcggcttc tgtgatatca gaatgatcca aaagcaacca gcctaatgca ctagaaggcg
      481 catgtgaggt cttaaggtat gaataacata gaaaaaataa gatcgtcaaa gttgcaaaaa
      541 aagagcctaa atgtaaaaaa ttagtggttc tacgttgaat ttcca
//
LOCUS       DAZKLD010000311          573 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.320, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000311 DAZKLD010000000
VERSION     DAZKLD010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..573
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.320"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>573
                     /locus_tag="ACG8KR_003020"
     CDS             <1..>573
                     /locus_tag="ACG8KR_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS982-like element ISEfm1 family transposase"
                     /protein_id="HFQ0202290.1"
                     /translation="IACVIWGIINGYTSQRATYRAVCSVLFPNGDFPSRSRFTRLSSN
                     LAYTIKIIRYFFIKKLTKGELVGIIDSFPSPLCKPVRNRQAKLLNQIAKVGYNSTKKS
                     YFYGLKIHMIVTKTGFPITYSITNPGVHDVKVLETLSEEANLPNILGDKGYISHKIHE
                     KLALKGITISVPPRKNMDKSEKLDHSLLGKQ"
BASE COUNT          195 a          110 c          100 g          168 t
ORIGIN      
        1 attgcttgtg ttatatgggg cataattaat ggctatacta gccaaagagc cacgtataga
       61 gcggtttgtt ccgtcttatt tcctaatggt gactttccta gtaggagtcg attcactcgc
      121 ttaagttcaa acttagctta cactatcaag attattcgat actttttcat caagaagctc
      181 accaaaggtg aactagtcgg aattatagat agttttccta gtccattatg taaaccagtt
      241 agaaatagac aagcaaaact attgaatcaa atagccaaag ttggctataa ctcaacaaaa
      301 aaatcttatt tttatggtct taaaattcat atgatcgtta ctaagactgg ctttccgatt
      361 acttactcaa tcacgaatcc aggtgtccat gacgtgaaag tgttagagac tttatcggaa
      421 gaagcgaatc ttcccaatat cctaggggac aaaggatata ttagccataa aatccatgaa
      481 aagcttgccc ttaagggcat tactatatcc gttccgccac gtaaaaacat ggataaatca
      541 gaaaaactag accacagctt acttggaaaa caa
//
LOCUS       DAZKLD010000312          561 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.321, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000312 DAZKLD010000000
VERSION     DAZKLD010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 561)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 561)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..561
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.321"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..381
                     /locus_tag="ACG8KR_003021"
     CDS             <1..381
                     /locus_tag="ACG8KR_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFQ0202291.1"
                     /translation="DYLSPIENIRLVNKSADESILFELGLDKKQIKRNVMKLSGGQQQ
                     RVAIARALVSDAPIILADEPTGNLDSVTAGEIINILKTLAKDRNKCVIVVTHSKEVAD
                     SADIILELSGKKLKKVNKMNLEVE"
     gene            381..>561
                     /locus_tag="ACG8KR_003022"
     CDS             381..>561
                     /locus_tag="ACG8KR_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0202292.1"
                     /translation="MIKNAFAYVTRKSLKSLIIILVILSMSTLSLISLSIKDATDRAS
                     KETFANITNSFSMEIN"
BASE COUNT          231 a           57 c           96 g          177 t
ORIGIN      
        1 gattatttat caccaattga aaatattaga ttagtaaata aatcagcaga tgaaagtatc
       61 ttgtttgaac taggtttaga taaaaaacaa ataaaaagaa atgttatgaa attatctggt
      121 ggacagcaac aaagagtagc tattgctaga gcattggtat cagatgctcc aataatacta
      181 gctgatgagc ctactggtaa tctagacagt gttactgctg gagaaataat taatatatta
      241 aagacattag ctaaagatag aaataaatgt gtaatagttg taacacatag taaggaagta
      301 gcagattctg cggatatcat tttagaacta agtggtaaaa agttgaaaaa agtaaataaa
      361 atgaatttgg aggttgaata atgataaaaa atgcatttgc ttatgtaact agaaaaagtt
      421 taaaatcatt aattatcata ttagttattt tatctatgtc aactttaagt ctcattagtt
      481 tatctattaa agatgctacg gacagagctt caaaagaaac atttgctaat ataacaaata
      541 gtttttctat ggagataaat a
//
LOCUS       DAZKLD010000313          558 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.322, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000313 DAZKLD010000000
VERSION     DAZKLD010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 558)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 558)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..558
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.322"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            155..316
                     /locus_tag="ACG8KR_003023"
     CDS             155..316
                     /locus_tag="ACG8KR_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HFQ0202293.1"
                     /translation="MQDKDKCINCGKCKKVCQMNKDSVENCNHLECIRCGRCKNACPV
                     DAISYGVRK"
     gene            335..>558
                     /locus_tag="ACG8KR_003024"
     CDS             335..>558
                     /locus_tag="ACG8KR_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0202294.1"
                     /translation="MIKNAIAYITRKKNRTLIIFIILTIVLSCLYSCLTIMKSSNEIE
                     KALYESSNSSISITKKDGKYFNVNQFKDIE"
BASE COUNT          204 a           62 c          103 g          189 t
ORIGIN      
        1 ctgcgcaagg cttaccagcg agatagagat cgacgatttg ttgtttgaat tcttttgaat
       61 aggttcttcg ttgacgtctt ggcatgaaaa atcctccagt gtgtttttta ttattctaca
      121 caccttattt tttctgtcta gtctagtgta gccgattcag gataaggata agtgtattaa
      181 ttgtggaaaa tgtaaaaaag tttgccaaat gaataaagat tcagttgaga attgcaatca
      241 tctggagtgt atcagatgtg gacgatgtaa aaatgcttgt ccagtggatg caattagtta
      301 tggagtaaga aaatagaaag gagtagttag gaaagtgata aaaaatgcta tagcatatat
      361 tacaagaaag aaaaatagaa ctttaataat ttttatcata ttaactatag ttctttcttg
      421 cttgtattct tgtttaacaa taatgaaatc aagcaatgaa atagaaaagg ctttatatga
      481 aagttctaat tcttcaatat caataacaaa aaaagatggt aaatatttta atgttaatca
      541 atttaaagat attgaaaa
//
LOCUS       DAZKLD010000314          558 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.323, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000314 DAZKLD010000000
VERSION     DAZKLD010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 558)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 558)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..558
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.323"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..434)
                     /locus_tag="ACG8KR_003025"
     CDS             complement(<1..434)
                     /locus_tag="ACG8KR_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0202295.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYE
                     EVKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRF
                     KSKKNPVQSYTTNNQNGTVALIDSKFIKVPKLKSLVRIKLHR"
     gene            complement(451..>558)
                     /locus_tag="ACG8KR_003026"
     CDS             complement(451..>558)
                     /locus_tag="ACG8KR_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFQ0202296.1"
                     /translation="WKEMFWSKSFCLLTTGGAPIDVIKKYIQNQGNNHK"
BASE COUNT          164 a           96 c           86 g          212 t
ORIGIN      
        1 tgtctatgaa gcttaattct tactaatgac ttcaatttag gaactttgat gaatttacta
       61 tcaatcaaag caactgtacc attttgatta ttcgttgtat aactctgaac aggatttttc
      121 ttacttttga aacgtggaaa tccaacggat ttatcccgaa aaaaattctt gtatgcttta
      181 tctaaattaa gttgggcatt ggctaaagca aggctatcaa tttctttcaa aaacggaaat
      241 tctttcttgt atttagctgg tgtcggaaaa gtcatttttt tagaagaatc atttttaact
      301 tcttcatacg ccttttttcg gtcatcaagc attagattgt agaccttacg gacacaacca
      361 aaagatttgg caaagaagat ttcttgttcc tctgttggat agattctgaa tttgtatgct
      421 tttagttgtt ccataaagtt cacctacttt ctatttatga ttgtttcctt gattttggat
      481 atacttctta atcacatcaa ttggtgcgcc accagttgtc agaagacaaa aacttttaga
      541 ccagaacatt tctttcca
//
LOCUS       DAZKLD010000315          544 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.324, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000315 DAZKLD010000000
VERSION     DAZKLD010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 544)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 544)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..544
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.324"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            199..>544
                     /locus_tag="ACG8KR_003027"
     CDS             199..>544
                     /locus_tag="ACG8KR_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0202297.1"
                     /translation="MWLEKINDKKFKYTERYTDPLTEKKRKVSVTLSSNSRQAWNQAN
                     LLLNEKIAEKIKRNEELPLTFGELKTKWDEKYKPTVKESSYRTTQVYLSLISKYIKDD
                     VLVKNVNSNLIQD"
BASE COUNT          241 a           70 c           80 g          153 t
ORIGIN      
        1 atttttcaaa gtttcactag acgaaataga gaaagttgtt ttagaaaatt ataatggaac
       61 agtaacattt acaaaattag ctaaagctga acaatataga cgatctttag aattgtcaaa
      121 agattaaata tataaaaagc cttcgggctt tttcttttaa caattaatag gaacgtatat
      181 tcgaaaggaa tagaaataat gtggttagaa aaaataaatg acaaaaaatt taagtacacc
      241 gaacgttata cggatccctt aactgaaaaa aagagaaaag tgtccgttac tttatctagc
      301 aattcacgcc aagcttggaa tcaagctaac ttacttttga atgaaaaaat cgcagaaaaa
      361 ataaaacgaa atgaagaatt accactaact tttggagaat taaaaactaa atgggatgaa
      421 aaatacaagc caactgtaaa agaaagttcc tatcgaacaa ctcaggttta tctatcatta
      481 atttcaaaat atataaaaga tgatgtactt gtaaaaaacg ttaatagtaa tcttatacaa
      541 gata
//
LOCUS       DAZKLD010000316          521 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.325, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000316 DAZKLD010000000
VERSION     DAZKLD010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 521)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 521)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..521
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.325"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>521
                     /locus_tag="ACG8KR_003028"
     CDS             <1..>521
                     /locus_tag="ACG8KR_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rib/alpha-like domain-containing protein"
                     /protein_id="HFQ0202298.1"
                     /translation="ENPGDNIPAGYHKVTFTAGEGTSIESGTTVFAVKDGVSLPEDKL
                     PVLKAKDGYTDAKWPEEATQPITADDTEFVSSATKLDDKSDADKYNPEGQKVTTELNK
                     EPDAFEGIKNKEDLPKDTKYTWKEKVDVSAAGNKKGTVVVTYPDGSSDEVEVDVTVTD
                     NRSDADKYEPTVEG"
BASE COUNT          209 a           81 c          121 g          110 t
ORIGIN      
        1 gaaaacccag gggataacat tccggctggc tatcataaag taacctttac tgcaggagaa
       61 ggaacaagta ttgaaagtgg aacaacagtc tttgcagtga aagacggcgt aagcttacca
      121 gaagataaac ttccagtgtt gaaagcaaaa gatggttata cagatgcgaa atggccagaa
      181 gaagcaactc aaccaattac agcagatgat acagaatttg tatcaagtgc aacaaaactg
      241 gatgataaat ctgacgctga caaatataat cctgaaggtc aaaaagtgac tacagaattg
      301 aataaggaac cagacgcatt tgagggaatt aagaacaaag aagatttacc aaaagatact
      361 aagtatactt ggaaagaaaa agtagatgtt agcgcagctg gaaataaaaa agggactgtt
      421 gtagtgacat atccagatgg atcatctgat gaagttgaag tagatgttac agtgacagac
      481 aatcgttctg acgccgataa atatgagcca acagtagaag g
//
LOCUS       DAZKLD010000317          511 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.326, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000317 DAZKLD010000000
VERSION     DAZKLD010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 511)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 511)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..511
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.326"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..342
                     /locus_tag="ACG8KR_003029"
     CDS             <1..342
                     /locus_tag="ACG8KR_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001226076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0202299.1"
                     /translation="DNIRDKKASLKSLKDTWLDLSEDNPYSQFLITVMAGVNQLERDL
                     IRMRQREGIELAKKEGKFKGRLKKYHKNHAGMNYAVKLYKEGNMTVNQICEITNVSRA
                     SLYRKLSEVNN"
BASE COUNT          206 a           68 c           93 g          144 t
ORIGIN      
        1 gataacatac gagataaaaa ggcaagttta aaatcactaa aagatacatg gcttgattta
       61 tcagaagata atccatacag ccaattctta attactgtaa tggctggtgt taaccaatta
      121 gagcgagatc ttattcggat gagacaacgt gaagggattg aattggctaa gaaagaagga
      181 aagtttaaag gtcgattaaa gaagtatcat aaaaatcacg caggaatgaa ttatgcggta
      241 aagctatata aagaaggaaa tatgactgta aatcaaattt gtgaaattac taatgtatct
      301 agggcttcat tatacaggaa attatcagaa gtgaataatt agccattctg tattccgcta
      361 atgggcaata tttttaaaga agaaaaggaa actataaaat attaacagcc tcctagcgat
      421 gccgaaaagc cctttgataa aaaaagaatc atcatcttaa gaaattctta gtcatttatt
      481 atgtaaatgc ttataaattc ggccctataa t
//
LOCUS       DAZKLD010000318          509 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.327, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000318 DAZKLD010000000
VERSION     DAZKLD010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..509
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.327"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          228 a           70 c           81 g          130 t
ORIGIN      
        1 aattccttag gcagcttttg ctgtgtagaa aggaataaat aataacgaaa gttcaaaagc
       61 taatgccgac tattctagac attaaagaag acgaaaacga acgataaaga aagaaaagtt
      121 atgaataatc gtttattgaa aaaaacttga aaaaatacaa aaaattcgtt gaccaagcta
      181 aatttagttg gtatattatt acttgtccaa aagacagaag aaaatattta ttcaaaaaca
      241 taacaaaaaa gttattgaca tgttactaac gacatgataa gataactgag tcgttaaaaa
      301 agaaccactt gcgaagcttg attctatcga aaaaaacttc aaaaaagttc ttgacaaata
      361 acaaacgata agttatgata aatgagttgc cgaaagattt cgaaaaacga aatcgaaaaa
      421 aacttttaaa aaagagttga caatcgcttg tctctctgat atgatataaa agttgctacg
      481 gcagccaaaa atgaatcggg aaacactct
//
LOCUS       DAZKLD010000319          508 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.328, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000319 DAZKLD010000000
VERSION     DAZKLD010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..508
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.328"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..508
                     /locus_tag="ACG8KR_003030"
     rRNA            <1..508
                     /locus_tag="ACG8KR_003030"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          122 a          111 c          159 g          116 t
ORIGIN      
        1 agtgatccgg tggttccgca tggaagggcc atcgctcaac ggataaaagc taccctgggg
       61 ataacaggct tatctccccc aagagtccac atcgacgggg aggtttggca cctcgatgtc
      121 ggctcgtcgc atcctggggc tgtagtcggt cccaagggtt gggctgttcg cccattaaag
      181 cggcacgcga gctgggttca gaacgtcgtg agacagttcg gtccctatcc gtcgcgggcg
      241 ttggaaattt gagaggagct gtccttagta cgagaggacc gggatggact taccgctggt
      301 gtaccagttg ttctgccaag ggcattgctg ggtagctatg tagggaaggg ataaacgctg
      361 aaagcatcta agtgtgaagc ccacctcaag atgagatttc ccatttcttt aagaaagtaa
      421 gatccctgag agatgatcag gtagataggt caggagtgga agtacagtga tgtatggagc
      481 ggactgatac taatcgatcg aggactta
//
LOCUS       DAZKLD010000320          507 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.329, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000320 DAZKLD010000000
VERSION     DAZKLD010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..507
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.329"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>507
                     /locus_tag="ACG8KR_003031"
     CDS             <1..>507
                     /locus_tag="ACG8KR_003031"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0202300.1"
                     /translation="LKKHYTEPWEDEVFRVIIEYNNVPIGYGQIYKMYDELYTDYHYP
                     KTDEIVYGMDQFIGEPNYWSKGIGTRYIKLIFEFLKKERNANAVILDPHKNNPRAIRA
                     YQKSGFRIIEDLPEHELHEGKKEDCYLMEYRYDDNATNVKAMKYLIEHYFDNFKVDSI
                     EIIGSGYD"
BASE COUNT          207 a           46 c           93 g          161 t
ORIGIN      
        1 cattaaaaaa acattataca gagccttggg aagatgaagt ttttagagta attattgaat
       61 ataacaatgt tcctattgga tatggacaaa tatataaaat gtatgatgag ttatatactg
      121 attatcatta tccaaaaact gatgagatag tctatggtat ggatcaattt ataggagagc
      181 caaattattg gagtaaagga attggtacaa gatatattaa attgattttt gaatttttga
      241 aaaaagaaag aaatgctaat gcagttattt tagaccctca taaaaataat ccaagagcaa
      301 taagggcata ccaaaaatct ggttttagaa ttattgaaga tttgccagaa catgaattac
      361 acgagggcaa aaaagaagat tgttatttaa tggaatatag atatgatgat aatgccacaa
      421 atgttaaggc aatgaaatat ttaattgagc attactttga taatttcaaa gtagatagta
      481 ttgaaataat cggtagtggt tatgata
//
LOCUS       DAZKLD010000321          503 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.330, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000321 DAZKLD010000000
VERSION     DAZKLD010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 503)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 503)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..503
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.330"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..174
                     /locus_tag="ACG8KR_003032"
     CDS             <1..174
                     /locus_tag="ACG8KR_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010868911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HFQ0202301.1"
                     /translation="HRSIKRRHKLYQSLRTASTTIKGMETIRGIYKKSRKEGTLFGFS
                     VCTEIRILLGIPA"
     gene            complement(171..449)
                     /locus_tag="ACG8KR_003033"
     CDS             complement(171..449)
                     /locus_tag="ACG8KR_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0202302.1"
                     /translation="MKTINLIKVFIASPGDIAAKRNEVEETIYRWNIENTDNTKVILM
                     PIRWETNAIPTYVNNSSGQSVINKQIVENRVLLQSFKNKISFCIISSF"
BASE COUNT          160 a           90 c           68 g          185 t
ORIGIN      
        1 catcgatcga taaaacgtcg acataaattg tatcaaagtc tacgtacagc ttccaccaca
       61 attaaaggga tggaaaccat tcgaggaatc tacaagaaaa gccgaaaaga aggcactctc
      121 ttcggctttt cagtctgcac tgaaatcagg atattattgg gaattccagc ttaaaatgaa
      181 gaaataatac aaaaactgat tttattttta aaactttgca gcagaaccct attttctaca
      241 atttgtttat taataacact ttgcccagag ctattattta cataagtagg aatcgcatta
      301 gtttcccatc gtataggcat caaaatcact tttgtattat ctgtattttc tatattccat
      361 ctatatatgg tttcttctac ttcattcctt tttgctgcta tatcacctgg tgatgctata
      421 aatactttta taagatttat ggttttcatt tattgtacct ttctctctag ttctctttaa
      481 agtaatttta gtataaaatt tat
//
LOCUS       DAZKLD010000322          501 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.331, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000322 DAZKLD010000000
VERSION     DAZKLD010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 501)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..501
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.331"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>501
                     /locus_tag="ACG8KR_003034"
     CDS             <1..>501
                     /locus_tag="ACG8KR_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
                     /protein_id="HFQ0202303.1"
                     /translation="MSYNEVVTITPRNYRFDFRKVKNLDKHYAMIGIAAGVSNYPLYY
                     EATNEKGLSMAGLNFPGNADYKELQEGKDNVAPFEFIPWILGQCSTIDEAKELLATIN
                     LVNIDFSEKLPLSPLHWLLADKEKSIVIESMKDGLHLYDNPVGVLTNNPPFDYQLFNL
                     NNYRSLS"
BASE COUNT          177 a           77 c           92 g          155 t
ORIGIN      
        1 gatgtcttat aatgaagtgg tcactattac tccaagaaat tatcgtttcg attttagaaa
       61 ggtaaaaaat ttagataaac attatgcaat gataggaata gcagctgggg tatccaacta
      121 tccactctat tatgaggcta caaatgaaaa agggttgagt atggcaggac tgaattttcc
      181 tggcaatgct gattataaag aattacaaga aggaaaagat aacgttgcac catttgagtt
      241 tattccatgg atactagggc aatgctcaac catagacgaa gctaaagaat tattggctac
      301 tatcaatcta gtcaatatcg attttagtga aaaactaccc ttgtcacctt tacactggtt
      361 attagctgat aaagaaaaat cgatagtaat tgaaagtatg aaagatggac ttcatttata
      421 tgataatcct gtcggtgtgt tgacaaataa tccgccattt gattatcaat tatttaatct
      481 gaacaattat cgctcgctat c
//
LOCUS       DAZKLD010000323          493 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.332, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000323 DAZKLD010000000
VERSION     DAZKLD010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..493
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.332"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            258..493
                     /locus_tag="ACG8KR_003035"
                     /pseudo
     CDS             258..493
                     /locus_tag="ACG8KR_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611995.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
BASE COUNT          169 a           88 c           69 g          167 t
ORIGIN      
        1 ttttacactc tctattattc ttttcataaa tattgtaaat agaagtatta aaaatatata
       61 caaaaagtat tatttcgaga aagataaata ttttcaaaaa ccttttcata atcctcaccc
      121 tttcttacac tgataaatca tgcgtctaat attcaaatta tactcgatat gctttagcta
      181 atcagccaac tctgtttgtc tactatcatt atatcaatag acaatatctg ctcccgtctt
      241 tatgatcgtg ccaaagcaat caaaagaaaa ttgcgaatca cttagatcga aagtttactc
      301 aaacaaaagc attgtcagcg gttgtcacgg atttaactta tgttcgggtc gggacaaatt
      361 gggcttatat ttgtttgatg attgatttat tcaatcgtga aattattggc cattcttgcg
      421 gaaataaaaa agatgcgcag cttgtaaaaa gggccattca aagcattcct tactcacttc
      481 aagaaatcga act
//
LOCUS       DAZKLD010000324          487 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.333, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000324 DAZKLD010000000
VERSION     DAZKLD010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 487)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 487)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..487
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.333"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>487)
                     /locus_tag="ACG8KR_003036"
     CDS             complement(<1..>487)
                     /locus_tag="ACG8KR_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFQ0202304.1"
                     /translation="IHRRFYTSICHQKITTDTTEFKYHEVDAKGIIRQKKLYLDPFMD
                     MFNSEILSYRISEKPNALAVMEGLEEAIQTTNDCPYRRTFHSDQGWAYQMKAYRNKLK
                     EYKIFQSMSRKGNCLDNSLMENFFGLLKQEIFHGKVYNCFEELKSAIDSYIYYYNNER
                     IK"
BASE COUNT          142 a           92 c           86 g          167 t
ORIGIN      
        1 gttttatccg ttcattattg tagtaataga tatagctatc gatggctgat tttaattcct
       61 cgaaacagtt ataaactttc ccgtgaaaaa tctcttgttt taataagcca aaaaaattct
      121 ccataagtga attatctaag cagttccctt tccgagacat gctttgaaag attttgtact
      181 ccttcaattt gtttctataa gctttcattt gatacgccca accttgatct gagtgaaacg
      241 tacgacgata gggacaatcg ttagtggtct ggatagcttc ctccaaacct tccattaccg
      301 ctaatgcatt gggcttttca gagatacgat aggataaaat ctcactattg aacatatcca
      361 taaaaggatc tagatacaat ttcttttgac ggataatgcc tttggcatcc acttcatggt
      421 atttaaattc agttgtatcc gtcgtgattt tctgatggca gatactggtg tagaaacgac
      481 gatggat
//
LOCUS       DAZKLD010000325          436 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.335, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000325 DAZKLD010000000
VERSION     DAZKLD010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 436)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 436)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..436
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.335"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(45..>436)
                     /locus_tag="ACG8KR_003037"
     CDS             complement(45..>436)
                     /locus_tag="ACG8KR_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFQ0202305.1"
                     /translation="EDILKKYEILANQKKITVNNYILDENVYIGKTALKIILSNLISN
                     AVKYTDVNGVINIGIVNDWLYIENSYGNNKISNMDKIFDVKFDLNKENSNGLGLYIVS
                     NILNNYNIEYKVLQDEEFFIFKIKIFS"
BASE COUNT          156 a           66 c           23 g          191 t
ORIGIN      
        1 ataagtctct atttttcaat aactattgca aaattatatc ttaattaaga aaatattttt
       61 attttaaaga taaaaaattc ttcatcctgc aatactttat attctatatt gtaattattc
      121 agaatattac ttacaatata taaacctaat ccattactat tttccttatt taaatcaaat
      181 ttaacatcaa atattttatc catattcgaa attttattgt taccgtatga attctctata
      241 tataaccaat cattaactat cccaatatta attaccccat ttacatcagt atattttacc
      301 gcattactaa tcaaattaga tagaataatc tttaaagctg tttttcctat ataaacattc
      361 tcgtctaata tataattatt gacagttatt ttcttttgat ttgctaatat ttcatacttt
      421 tttaatatgt cttcta
//
LOCUS       DAZKLD010000326          429 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.336, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000326 DAZKLD010000000
VERSION     DAZKLD010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..429
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.336"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>429
                     /locus_tag="ACG8KR_003038"
     CDS             <1..>429
                     /locus_tag="ACG8KR_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HFQ0202306.1"
                     /translation="LIEYTENLSKTIEQIKIDNLLSQNYDPEIYLFLYENKILEYVKN
                     GDIRNLENMVFNLSNGIIPSVSGDTIRSEKNYSIIVFEKLAQTSITLGMDIIEAYQSR
                     DALIQENELAVSLPEVLKVRDSGIVYYTKEIGKTKIEHLSP"
BASE COUNT          170 a           49 c           77 g          133 t
ORIGIN      
        1 gttaattgaa tatacagaaa atctaagtaa aactatcgag caaatcaaaa tagataatct
       61 gttatcacaa aattacgatc cagaaatata tctgtttctt tatgaaaata aaatactgga
      121 atatgttaaa aatggagata ttcgaaattt agaaaacatg gtttttaatc tgagtaatgg
      181 aataattcca agtgttagtg gagatacgat tcggtctgaa aaaaattatt ctatcattgt
      241 ttttgaaaag ttagcacaaa cgtctataac attaggaatg gatataatag aagcgtatca
      301 aagtagagat gctttgatac aagaaaacga attagctgta tcgttacctg aggtcttaaa
      361 agtgagggat tctgggattg tttattatac taaggaaatc ggaaaaacta agatagagca
      421 tctttctcc
//
LOCUS       DAZKLD010000327          416 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.337, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000327 DAZKLD010000000
VERSION     DAZKLD010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..416
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.337"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>416
                     /locus_tag="ACG8KR_003039"
     CDS             <1..>416
                     /locus_tag="ACG8KR_003039"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFQ0202307.1"
                     /translation="KDNRVRVIHKENGGLSSARNAGIDVARGKYLGFVDSDDYIDEDM
                     YEILYENLKIHDADISSVELIPFYGDRYKKANKEKKVIILNKKEAIKSVLEGTQFYAY
                     AWNKLYRKELFKDNRYLDGKTFEDAYIIIDLLFQTE"
BASE COUNT          163 a           40 c           86 g          127 t
ORIGIN      
        1 aaagataatc gagtacgtgt gatccataaa gaaaacggcg gattaagcag tgctagaaat
       61 gcggggattg atgttgcgag aggcaaatac ttaggctttg ttgacagtga tgattatatt
      121 gatgaagata tgtatgaaat tttgtatgag aatttgaaaa ttcatgatgc cgacattagc
      181 tctgttgaat tgattccttt ttatggtgat agatataaga aagctaataa agaaaagaaa
      241 gtaattatat taaataaaaa ggaagcaata aaatctgttt tagaaggaac gcaattttat
      301 gcatacgcat ggaataaact ttatcgtaag gaacttttta aagataatcg ttacttggat
      361 ggaaaaacat ttgaggatgc atatataata atagatctat tatttcaaac agaaaa
//
LOCUS       DAZKLD010000328          408 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.338, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000328 DAZKLD010000000
VERSION     DAZKLD010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..408
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.338"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>408)
                     /locus_tag="ACG8KR_003040"
     CDS             complement(<1..>408)
                     /locus_tag="ACG8KR_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFQ0202308.1"
                     /translation="DTNVAYRWFLGYSFEEKIPHFSTFGKNYVRRFRETTVFEDIFAY
                     ILEQAVKAGFVTEDNLYLDSTHIKANANKHKFTKEMTHDEAKAYQDELEDEINRQRIE
                     AGKRPFTLDLEKEVKLKERKISKADPESGYYVKG"
BASE COUNT          104 a           89 c           72 g          143 t
ORIGIN      
        1 cctttgacgt agtagccgct ttccggatca gctttactta tttttctttc tttcagcttc
       61 acttcctttt ctaagtccaa agtaaaaggg cgttttccag cctctattcg ttgacgattg
      121 atctcatctt ctaattcatc ctgataggct ttcgcttcat catgggtcat ttctttcgta
      181 aacttatgtt tattggcgtt agctttgatg tgggtagaat ccagatacaa attatcctct
      241 gttacaaaac ctgctttaac tgcttgttct aaaatatagg caaaaatatc ctcaaacacc
      301 gtcgtttctc taaatcggcg aacataattc tttccaaaag ttgagaaatg ggggattttc
      361 tcctcgaagg agtagcctaa aaaccagcgg taagcgacat tggtatcg
//
LOCUS       DAZKLD010000329          405 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.339, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000329 DAZKLD010000000
VERSION     DAZKLD010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..405
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.339"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          168 a           64 c           48 g          125 t
ORIGIN      
        1 gaataagatt agaaaataac accccgagcc aatctgtgaa aattactata tttactaata
       61 tccctattaa catacctatc atatttatta aatcaatttt tttcattaga tatttctcct
      121 ttattaaatc tgaagtactt atattaaaat aattctaaac tatttacctc aaaaagtgca
      181 ataaattatt tctgaacaat aaaatgatag tcaaaacaaa aaaatattgt taatcgttca
      241 gagccatccg agagtgtaaa atattttgtg taaatgaaaa aatccataca aaaaaggaag
      301 gcgcttctgt agaataaagt taacgacaac caattcacag aaaagaggac ttccctatga
      361 atgattttac tacagaaatt gtgcaaactc tagtcactaa aggcg
//
LOCUS       DAZKLD010000330          374 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.340, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000330 DAZKLD010000000
VERSION     DAZKLD010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..374
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.340"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            160..270
                     /locus_tag="ACG8KR_003041"
                     /pseudo
     CDS             160..270
                     /locus_tag="ACG8KR_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          126 a           59 c           43 g          146 t
ORIGIN      
        1 atctttcagc attttactat atttggtata tagtgctaag gttcatattc gattgacaag
       61 agaatataaa ttgatctatt tctatttcct aagttgagta aacataactt tgccctttat
      121 aaatatcttc cctctctctc ttctctctat attttatatt tgaataatca tatcaaggta
      181 ttccaacgaa atgccaatgg atttcgaagc ttttataact ttaaattacg gatcatgatt
      241 tggcatgggg aatcattact aataaaataa aaaaatgaac agaaagaaaa cagttctttc
      301 tgttcaccaa cttcatttga caaatatcct atttagctta ttttatagct ttttatatac
      361 acttttttaa aaag
//
LOCUS       DAZKLD010000331          370 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.341, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000331 DAZKLD010000000
VERSION     DAZKLD010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 370)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 370)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..370
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.341"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..202)
                     /locus_tag="ACG8KR_003042"
     CDS             complement(<1..202)
                     /locus_tag="ACG8KR_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0202309.1"
                     /translation="MRLYHLRSLEELRKLDFNKKFFWEISDLSCFVESERLSNGNNSV
                     LNEYDKVVINKVKSLCEEARESI"
     gene            complement(219..>370)
                     /locus_tag="ACG8KR_003043"
     CDS             complement(219..>370)
                     /locus_tag="ACG8KR_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFQ0202310.1"
                     /translation="LKSNHTISEVSLGLGFSDSSHFSRVFKKYAGVSPKQYQLGLVDN
                     FNNIS"
BASE COUNT          132 a           73 c           38 g          127 t
ORIGIN      
        1 tgatagattc tctagcttct tcacataaac tttttacttt attaattact actttgtcat
       61 attcatttaa cacactatta ttaccattac ttagacgctc agattctacg aaacaactca
      121 aatcagaaat ttcccaaaaa aattttttgt taaaatccaa tttcctcaac tcctccaaac
      181 tacgtaaatg atatagtctc ataataaaat aaaacctctc agctaatgtt gttgaaattg
      241 tccactaaac ctaattgata ttgcttagga gatacacctg cgtacttttt gaatactctc
      301 gaaaagtgtg aagaatctga aaatcctaaa cctaaagaaa cttcactgat agtatggttt
      361 gatttcaaca
//
LOCUS       DAZKLD010000332          367 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.342, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000332 DAZKLD010000000
VERSION     DAZKLD010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 367)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 367)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..367
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.342"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          123 a           52 c           47 g          145 t
ORIGIN      
        1 aaaaatcgtc atttttccat caaaatctgc ttatgcaaaa ttataaaaaa attttgcagt
       61 ttttcttact ttttttgtcg tttaattgaa tgataaaaac cagtttcttt attcagttaa
      121 tcaaatatga acgaattaga tatgaatcat tgtatttgcc gttgcggaag gattttttaa
      181 ttaaccacag ataaacattg ataacgattc gcttattgta atcaacagat taaatttcat
      241 atgttcatat gtacggatac attttcggac cgattaattt taaccattct gtttatttgc
      301 ttacctatta gatatcaata aaaaactgct taacagctat ttcagctgtt aagcagtttt
      361 aggattt
//
LOCUS       DAZKLD010000333          355 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.343, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000333 DAZKLD010000000
VERSION     DAZKLD010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..355
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.343"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..220
                     /locus_tag="ACG8KR_003044"
     CDS             <1..220
                     /locus_tag="ACG8KR_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="arsenate reductase"
                     /protein_id="HFQ0202311.1"
                     /translation="DYFTQADLIITLCGDAKDKCPAVPNNHEHLHWDLKDPAQAVGSE
                     EEILTEFRKVREGIKRKVLDLNQRTSIV"
BASE COUNT          133 a           69 c           62 g           91 t
ORIGIN      
        1 agattacttt actcaggcag acttgatcat tacactttgt ggtgatgcca aagacaagtg
       61 ccctgccgtt ccaaacaatc acgaacacct tcactgggac ctaaaagatc ctgcacaagc
      121 tgtcgggagc gaagaagaaa tattaacgga attcagaaaa gtcagagaag gaatcaaaag
      181 aaaagtttta gacttaaatc aacgtacatc gatcgtataa agaagtattc aaaatgattt
      241 ttcaagcact aagaaacaat gttaaaaaaa tagctttcca tattttacaa aattgtgcct
      301 tattgccgac aaattgtctc ttgaacaccg tttaccagta gtcaagacat cgtaa
//
LOCUS       DAZKLD010000334          345 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.344, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000334 DAZKLD010000000
VERSION     DAZKLD010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..345
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.344"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>345
                     /locus_tag="ACG8KR_003045"
     CDS             <1..>345
                     /locus_tag="ACG8KR_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000774078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0202312.1"
                     /translation="GVIANILFSCLNFRFTQKNKKNKLSIEQMKKDIETDLKNDHKER
                     RKIEEYTSLMEKCKATDAIFSFLRKSDEIITRENIDLENEISKLESIFSDINSLKYLI
                     VEENSDSFLEFEK"
BASE COUNT          149 a           35 c           53 g          108 t
ORIGIN      
        1 ggtgttattg ctaacatact tttttcgtgt ttgaatttta gatttactca aaagaacaaa
       61 aagaacaaac tcagtataga acaaatgaaa aaagacattg aaactgattt aaaaaatgac
      121 cataaagaaa gaagaaaaat tgaagaatat acttctttaa tggaaaagtg taaagcaact
      181 gatgcaattt ttagcttttt aagaaaaagt gatgagataa tcacacgtga aaatatagat
      241 ttagaaaatg agattagtaa acttgaatct atcttttccg atataaattc tcttaagtat
      301 ttaatcgtcg aagaaaattc ggatagcttt ttagaatttg aaaaa
//
LOCUS       DAZKLD010000335          343 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.345, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000335 DAZKLD010000000
VERSION     DAZKLD010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..343
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.345"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          122 a           43 c           57 g          121 t
ORIGIN      
        1 gataggcagt ggtatttctt cagtaagatt aaaagagaaa gaatatactc aaaagattat
       61 tcatattcct aagcctgaaa ttaagcgttt tgagataaaa gactattctg ctttagggct
      121 gttctcaatt ggttactggt gataaaaaat aagaatacta aaactatacg tagaatataa
      181 gtaatcggca tcaatcctaa ttatgtgatt gaatgccgat ttttgttacg cgctattttt
      241 aattatctgt aagaagaata attttatttt actcatattc gttttcactt tgaagaagta
      301 aattgcaatt tcttataagt taagcagcta tgcaaaaggc tat
//
LOCUS       DAZKLD010000336          342 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.denovo.346, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000336 DAZKLD010000000
VERSION     DAZKLD010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 342)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 342)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..342
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.denovo.346"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          131 a           53 c           72 g           86 t
ORIGIN      
        1 taatatacga attataagat acattttata tttaatgtat gtaaataaat aaaatgactg
       61 acggatattg aatagtaata aataacaata attcagacgg atacagtgag tgaagtagct
      121 tgctacgaac gaacaggcag aatgaaacgg tagtttcatt agagcaagtc gcttgcgacg
      181 tagcgttacc actgatgtaa ctggcaagtt acatagagag agtttgcttg caaacgaacg
      241 ttacccactt gaatagaaaa caaaaataac atattagtga attttgaata ggagccacaa
      301 ctcaaccaac cgcttgcggt gggtgagtcc cacggaggaa aa
//
LOCUS       DAZKLD010000337         7915 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.guided.132, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000337 DAZKLD010000000
VERSION     DAZKLD010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7915)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7915)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7915
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.guided.132"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(97..969)
                     /locus_tag="ACG8KR_003046"
     CDS             complement(97..969)
                     /locus_tag="ACG8KR_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFQ0202313.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKVFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            1097..1666
                     /locus_tag="ACG8KR_003047"
     CDS             1097..1666
                     /locus_tag="ACG8KR_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HFQ0202314.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            1733..2470
                     /locus_tag="ACG8KR_003048"
     CDS             1733..2470
                     /locus_tag="ACG8KR_003048"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFQ0202315.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            2701..3969
                     /locus_tag="ACG8KR_003049"
     CDS             2701..3969
                     /locus_tag="ACG8KR_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HFQ0202316.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            4456..4920
                     /locus_tag="ACG8KR_003050"
     CDS             4456..4920
                     /locus_tag="ACG8KR_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFQ0202317.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIEH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            4904..5533
                     /locus_tag="ACG8KR_003051"
     CDS             4904..5533
                     /locus_tag="ACG8KR_003051"
                     /EC_number="1.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016657 - obsolete oxidoreductase
                     activity, acting on NAD(P)H, nitrogenous group as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFQ0202318.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMQPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITTFE"
     gene            5887..7365
                     /gene="msr(C)"
                     /locus_tag="ACG8KR_003052"
     CDS             5887..7365
                     /gene="msr(C)"
                     /locus_tag="ACG8KR_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HFQ0202319.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRPDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            7412..7858
                     /locus_tag="ACG8KR_003053"
     CDS             7412..7858
                     /locus_tag="ACG8KR_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HFQ0202320.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
BASE COUNT         2640 a         1396 c         1624 g         2255 t
ORIGIN      
        1 aggaacaaat taagaaactt atgttattta atcattggat cttttgctta acttattcgt
       61 tgttaaaaag ctgaaagcga aggaaaaaaa ctctacttaa ctgtatttct ccgttaggac
      121 atctaaaaat ttacgagcag caggagaaag aggctggtct tttttccaaa tgagattcag
      181 tgaagcttgt aatggcggat cgagtgggat gaatactttt tccgtcccat aagtattgat
      241 gatcccatct agagctaatg caccaaaagt cccgttatcg accatcaaag aagcattgta
      301 caataagtta tatgttccga cgatcgataa ctgatctatg ctgaaaccta gccattcagc
      361 tagttgactg tccactgacg tttgccggga aatgatcaaa ggaacagttt tcagttcctg
      421 cggatggatg acggtttgct ttgctaaagg atgatttcga tgtatcaaaa gtccccaacg
      481 atcttgggct ggcagtcgaa atgactggta tttttgtttg tttgtcggtt cgattacgag
      541 tccaaaatca agtaatccgc tatccagttt gtctatgata tcatctgcat tcccgctata
      601 taaatgaaac tggatatcag gatgcgcctt gatcaacgaa tgaaggctat cagaaatgaa
      661 agagattgct ttagtttcgc ctccgccaat gtagatttct ccagagatga ttgcgtcatt
      721 tgttaaattg ttcgccgttt tttctaccaa cgacaagatt tcttttcctt ttgaataaag
      781 atatcgacca gaatccgtca actgtatctc tcgggagccc cgagtgaaaa gtgtcacacc
      841 aagttcttct tctaaatctt tcagttgttt tgagagagta ggttgcgtga tatgcagaga
      901 ttttgctgct cggctgatat ttttttctct agctactgct aaaaaatacg tcagtatacg
      961 aatatccatt ttttccctcc tgtttttaaa aaagcatacc actaagtata gttaaaagga
     1021 atagttaagt atgttttata agtattagac atgataagcc gtccgttata aagtaaggat
     1081 aaaaggaggg agcaaaatga caagcctcat cgaaaaacaa atcgtcaata aagagattcg
     1141 agtaacggat ccgctctttg aagtgatcca tcagatccaa gctgaaaacg aagagccttt
     1201 agcagcgctg aatactgggt accatgaacc agcagatatc cgcaaacggc tagaaaatat
     1261 cattagtgac aaagtagatg atacggtgac tgttctgctg cctttttata cagatttcgg
     1321 caaacatatt agtataggga aaaatgtctt tatcaatcgt caagtgatgt tcgttgactt
     1381 aggtggcatc tgtttagaag attcagtatt gattggtcct cgtgtcaacc tcatcacggt
     1441 gaaccatcta accgatccaa aagaacgaag aggcttgtca gtaaagccaa tccacatcaa
     1501 gaaaaatgct tggatcggag caggagcaac tattctgcca ggcgtaacga ttggcgagaa
     1561 tgccattgtt gcagcgaatg caactgttac gaaagatgtc ccagacaata ccatcgttgc
     1621 aggaatccct gccaaaatcg tcaaacctgt cgaacggatt atttgaacag taagatgaaa
     1681 taagagatag tgagtataag caagagattt ttcaaaacag gaggagaata gaatgaatga
     1741 tttatcagaa aaagtgattg tcatcatggg cgcttcaagc ggaatcggtg aagcaacaac
     1801 gaagctatta gcagaaaaag gggctaaact agttatagct gcacgccgag aagatcgcct
     1861 aaaagcaata aaagaatctt taccagaagc tgagctatat attcaaacag cagatgtaag
     1921 agattttgca caagtccaag cagttatcga tttagcgatg gaaaagttcg gtcgaatcga
     1981 tgtattgtac aataatgcag gaatcatgcc aaccgctcca ttagttgaag gtcaccgtga
     2041 tgaatggcaa aatatgcttg atatcaacat catgggtgtg ttaaatggaa tttccgccgt
     2101 acttccaatc atggaaaaac aaaaatctgg gcatatcatt tcaactgatt ctgtggcggg
     2161 acatgttgtt tatcctgatt ccgccgttta ttgcggaaca aaatttgctg tccgtgcaat
     2221 catggaagga ttacgtcaag agcaacggca aaataacatc aaatctacga tcatctctcc
     2281 aggagctgtt caaactgaat tatatcaaac catcagcgac aaaaaggctg ctcttgaact
     2341 tcatgaagcg caaaaagaat ggggattgac ctccgaagat atagcttctg ctgtagcatt
     2401 tgcgatagaa acgccagacc ggatgtctgt cagtaacatg attatccgtc cgacgacaca
     2461 ggaagtataa tccataacgg ataatatcca aagagaatta cttgttaaat agttattcaa
     2521 acgaccatca atttaaggcg tgaatggaga agaacattct tcatttacgt ctttttatgt
     2581 agaaaaagca aatttatcaa aaagaaacaa atgttattta aatatggaca ttgacttgct
     2641 tttacttttt tagttacact gttatttagg aaaagtttca taattcaaag gagaaaaaag
     2701 atgaaagcaa agaatttatt atttagcgtt attcttttag gaaatctagc agttgccaac
     2761 acaacagtgc tagctgaaga atcgacagaa actgtcgcaa ctcagacatc gagttcttca
     2821 gaaacaggaa ttgaaaacga tacagagagt actttgcagt cagctactgt ctcagaaaat
     2881 acaatttcaa ccgaagaacc aacaactgaa acaacagaac aaacacaaac cgaagacagt
     2941 acatcagcag aagctcagaa caatccagag aatgcagaag aaatcacgaa ccaattgaca
     3001 gatgggcaaa cattgacaca tacccatcaa gcagatttgt ctgaattcga aaactatact
     3061 ggctatgctt tttatgctcg ctcatctgct aattctcaac aagcgttcat tgatagtatt
     3121 gcctcaactg cacaaagttt ggcaagtgcc aatgacttat atgcatcagt catgattgct
     3181 caagccatcg ttgaaagtgg ctgggggaac agtactttgg cttctgcccc taattacaat
     3241 ctatttggga ttaaaggaag ctataatggc caatctgtta cgatgcctac ttcggaatat
     3301 gtgaatgggc aatggattac ggtgaatgcc gcttttagaa aatatccttc gtacaaagaa
     3361 tcattgcaag ataacgtaac ggtattgaag acgacttctt ttcagccagg cgtttactac
     3421 tattccggtg catggaaaag caatacgaac tcttacaaag atgctacggc ttggctaaca
     3481 ggtcgttatg ccactgcgcc taattatggt tctacattga acaatgtaat cgaaacctat
     3541 aatctgacac agtatgatac agcaccaact tcaacttctc cgatgtatcg tctgtataac
     3601 cgtcatacag gagaacattt atatacattg aatgccggag aaaaaaatta tctgccaact
     3661 gtaggctggg aatatgaagg gattgcttgg caagcaccaa acagcggaca acctgtctac
     3721 cgtttataca atccttacag tggagaccat cattacacaa tggcacaatc tgagatcaac
     3781 tctttaacaa aaatcggttg gcgttatgaa ggtctaagtt tttattctgg tggttccaag
     3841 ccaatttatc gactatttaa tccaaatgaa aaaactggta cccatcacta taccttgagt
     3901 gctaaggaaa gagattggct aacaccgatg ggctggagat atgaaggaat aggattttat
     3961 ggttattaaa aagaggtagt gaaaaagctg ccctgcatca agtagtaaga acagccaaac
     4021 aaaaacagat tttcatgttt tttgtttggt tgaacttatt accgcagatg caagtttttg
     4081 aacaccgttt actcaaaaaa ggttgtgaca acatcttgtc ataacttttt tattactgtt
     4141 taagcatact gatccaattt ctaatattcg acgggattct tgcaaaaggc gatttcggaa
     4201 atttttggcg gtgtaaaatt ttctactgca gaagagctat cttctatagt ttgataagaa
     4261 tcaataatct tggttttagc gttgtgcttt ctttatcatt ttttccgttc attctacaaa
     4321 aaatttcttt tctctatact tttctattga gagcgttttt tttattacac agctgattct
     4381 ttgacttttt tcttttttta ttctattatt gacaagtcaa tcgttgacga gtcaattatt
     4441 agaaaggagg aaattatgtc tttagaagag attagtcacc ttttatatca aatcaaatta
     4501 gccgatcaaa aaatcgtcca gatctttgaa gaaaatatcg gtataagtct tactagatac
     4561 gagatcatga tgagactgaa agaagaaaat ccattgctac aaagcagttt gcaagaaaag
     4621 ttgcagattg atcaaggagc agttactcgt catctgaagg tattggaagc aaatggctat
     4681 gtttcgcgtc agagaaatcc tcagaacaac cgggaagtgt atgtagcgct aacagaaaaa
     4741 gcccagaaga aaatcgagca ttgtgaaatg gaccataaag atacaggttc cgtgctatcc
     4801 ccggctttca gcgatgtaga aatgaaacaa tttctaacac ttttagataa attgaatacc
     4861 caactttgca atataacaac agaaacgaaa gaggttaata ataatggaac aaaaaaatga
     4921 tttttcaaca attatgactg gtagaagatc cgttcgcgta tatgatgaaa catacaaaat
     4981 cccacacgaa gaaatgctgg aaatgatcaa agaagcgacg actgcacctt cttctgtcaa
     5041 tatgcaacca tggcgctttg tggtcgctga aagcgaggaa gcaaaagaaa tcttgcgtcc
     5101 tttgatccgt ttcaatacga gacaaaatga tacttcttct gcaatggtca tgatttttgg
     5161 cgatatgcaa tgttacgaat atggcgaaga aatctataac caagcttacg aatcagggaa
     5221 gatgccaaaa gaagtcagag atcaacaatt agctgcgatc attccttatt acaagagttt
     5281 ttcaagagaa gaaatgaacg atgtagtcaa agtcgattct agcttagcgg ctatgcaatt
     5341 catgttagtt gcgcgtgatc atggctatga aacaaatcca attggtggat tcgaagcaga
     5401 tcagttggca gaggcatttg gtttggataa agaccgttat gttccagtta ttattttatc
     5461 aataggaaaa gcggtagaag aaggttacga gtctgttcgt ttagcaccag aaaagatcac
     5521 tacgttcgaa taaaaggttt tcagttacta ttcagtagct attgcttttt ttgaatcgtg
     5581 tgtggtaacc taataaaaaa ggaggtcgga aaaatgactg catcgatgaa attacgtttc
     5641 gaacttttga ataacaacta aataaattca aaagacatgt ataaacaaca atttcatgga
     5701 tggagtatgt ccattttttc gaacgacagt agaataggtg aaagctatga cagatttggt
     5761 gatttagtca tagttatttc gttcagagta ggaaaagatg tgatgcagtt tttttgcaaa
     5821 cagatttttc tgctcttttt tagtatcgat aattgttgtt ttacatgaaa taggagtgta
     5881 aaacatatgg aaaatttagc agtaaatata acaaatctac aagtcagctt tggcaaccag
     5941 ctagaactat ctattgattc tcttcgtgtc tatcagcaag atcgtatagg gatcatagga
     6001 gaaaatggtg tcggtaaatc gactttgctc aaactaatag ccggtgaact ttttcccgat
     6061 catgggaaaa tccaaacaga gatcaccttc aactacctgc ctcaattaac ctatctcgct
     6121 gaagcaaagg acctaaattt ggaattagcc agtcatttcc agttaagact ggaagaaact
     6181 tcagagcgga aatggagcgg aggggaagaa cgaaagatcg agttgatacg tcttctttct
     6241 tcttatgaac aagggatgct tctagacgag ccaacaaccc atctagatag aaaaagtatt
     6301 gatcgactga ttgaagagct tcgttattat tatggtacgc tggtttttgt tagtcatgat
     6361 cgctattttc tagatgagct agcatcgaaa atctgggaag taaaagacgg agaaatccga
     6421 gagttttcgg ggaattatag tgcctatctc actcaaaagg aattggagaa aaagactcag
     6481 ctacgagaag cagagtcgat catgaaagag aaaaaacgat tggaaaaatc gatccaagaa
     6541 aagaaaaaac aagcggaaaa gttggaaaaa gtgtccagta aaaagaaaaa gcaacaaatc
     6601 agaccggatc ggttgtcttc ctctaaacaa aaagacagtg tacaaaaagc catccaaaag
     6661 aatgcgaaaa cattagagag aagactccaa aaaataggag aaacaaccaa gccgcagcag
     6721 atgaaacaaa tccgttttcc agtaccaaaa tctcttgaac tccacagccg ctatcccatc
     6781 atgggacaaa atgtccaatt ggaaagaagt gggagaacat tactggtaaa tggtgatttt
     6841 cagttttctt taggtaaaaa aatcgcgatt gtcggtgaaa atggttcagg taagacaacc
     6901 ttattagaac atatccgcaa acaaggagaa ggaatccttc tctctccgaa agtaagcttt
     6961 caagtatatc agcaaaaggg ttatcaaatg acatctgaag aatccatcat tcgttttgtc
     7021 atgagacaaa cagagttttc agaatcgctt gtccgtagtt tgctgaatca cttagggttt
     7081 gctcaggaaa ctttgacgaa accgttatgt acattaagtg ggggagaagc gacccgtctg
     7141 acgattgctt tgctttttac taagccaagt aatgtgttgc tgttagatga accgactaat
     7201 tttattgata tggcaacgat cgaagcttta gagaagctaa tgcaaatata tccgggaacg
     7261 attttgttta cttctcatga ttcttacttt gtcgagcgta cggctgatga agtttatgaa
     7321 ataaaagggc agaaaataaa aaaagtactt acgagaaatt tttaaatgtg ctacgatgat
     7381 tttcgtgaca aatttaaagg agtggtacag attgcctaca aataaaaaag aaggaataat
     7441 ttttacaaca atgatgtgtt ttttgatggt cttggggatg agcgcgtata atttatggct
     7501 gcatcaagat cttcatttat caagtttgtt gattggtcta gtgcctggat tcgtggtggc
     7561 tttcattttg gatgtcttca tcatcggtgt gctggcgaaa aaaattgcct ttaaactacc
     7621 agtaaatcca gaaagcaagg tccaaatgat cttaactatt tcatgcttga tggttttagg
     7681 aatggtgaca tgtatgtctt tattcggtgt gatcatggaa caaggaatac ctgatcaatt
     7741 attctctgct tatatgaatg cttggaaaat gaatgtgatt gccgctttac cgttgcagtt
     7801 gatcatcgtt ggcccgcttt caagaagtgt gctaaaagca attcaaagta atgaatgatg
     7861 gaacactgtt atttgaaaaa aggccttggc atgctttttg tcatagtctc ttgaa
//
LOCUS       DAZKLD010000338         5792 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.guided.156, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000338 DAZKLD010000000
VERSION     DAZKLD010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5792)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5792)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5792
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.guided.156"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            178..1074
                     /locus_tag="ACG8KR_003054"
     CDS             178..1074
                     /locus_tag="ACG8KR_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HFQ0202321.1"
                     /translation="MNKNDVLLIAKNKSLYFFPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMDFSSSEPKTDLFFGATNNGRKERAEKYKQEFTKR
                     GVTTNYFILPLRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYQFYHSENIFLLQERDLDELPEFLHTPFQPIPSELLEFFDAENWAKR
                     FFTTDSAIYEQYEYDLSSVKNK"
     gene            complement(1217..2158)
                     /locus_tag="ACG8KR_003055"
     CDS             complement(1217..2158)
                     /locus_tag="ACG8KR_003055"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HFQ0202322.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVVLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNKHVDLIVALTKANQLDDMFRSIQSIITENTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEAKLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAEGM
                     VRTIVSEFASIAAKEGITLDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAVWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            2284..3405
                     /locus_tag="ACG8KR_003056"
     CDS             2284..3405
                     /locus_tag="ACG8KR_003056"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HFQ0202323.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALGGTWRTIDGMDLLFRQRKISYLLTNWQGKNTAVIESAQVLGLDLLDKP
                     TDQTIRLASSYALGELIKDTLLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTRKLHLDRRNVLVSKVKVIIAADVTNPYTGQQGAAWQFGRQKGGTDTTLAFLD
                     KQAEKIAQQIKDVWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFELVSQITGLEKQI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDRELEGLFDFLPIV
                     LSIQLGPIDLEEAMEHRQTLEKTEILGQTLSRFFNILPK"
     gene            3663..5165
                     /gene="eat(A)"
                     /locus_tag="ACG8KR_003057"
     CDS             3663..5165
                     /gene="eat(A)"
                     /locus_tag="ACG8KR_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection-like protein
                     Eat(A)"
                     /protein_id="HFQ0202324.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQAFVYFPQQTKDKERLTYYVLNEIMDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEIAKNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKKVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDALTMNSQVSHHKRLLSVENLQLGYENLLFEPVHF
                     TIEPHQRMAISGPNGAGKSSIIHYLLGTFNGKVVGEKSQPKHLTISYVRQNYEDNRGT
                     LAEFAKKNRVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYI
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLEKI"
     gene            5232..5759
                     /locus_tag="ACG8KR_003058"
     CDS             5232..5759
                     /locus_tag="ACG8KR_003058"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFQ0202325.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQALAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVVDRLNLPMTADERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECMEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELISYE"
BASE COUNT         1902 a         1023 c         1221 g         1646 t
ORIGIN      
        1 taattacaac tattcagaaa acttttgggc atattttaat ggaagaatga tttatttcat
       61 cgtattttca tagaaatgtt aaatttttat aaaaataata ggaagcgttt tactggcttt
      121 ttactattta aacaggctat cataggaaaa agtgacagag aaagtgaggc ggaacagatg
      181 aacaaaaacg acgtattgct aattgcgaaa aataaatcac tttatttttt tccttccatc
      241 cgctctttag atgtttggtc cggaaaagat ttactacagt cggaattcat taaatcagct
      301 cgacaacagt tggcaaatta tcagctgatc gtttttttag attatggatt tgatatggag
      361 atggctcgac gcgtgcgtcc atttaccaat gcaaaaatcg tgttattttt ttggaaccac
      421 tttaaagcag aacacgaggc gatgctgaag caggcacaag atgaaccggc aatcgatgag
      481 atttatcatt ttgatttttt agaagcgaaa gaacttggac taaaacataa tagttccttt
      541 tattcgaaac atatggattt ttcttctagc gaaccaaaga cggatctatt ttttggtgca
      601 acaaataacg gcagaaaaga acgggcagaa aaatataagc aggaatttac aaaaagaggt
      661 gttacgacca attactttat tcttccgctt cgaggaaatg aacaacctgg ataccttact
      721 tatgaagaat atctgaaaaa aaccggggaa tcgaggggga ttctagaact gttgcgggaa
      781 gggcagcaag gagtgacttt gcgaacgttt gaatctatgt attttcaaaa gaaactagtg
      841 acagataatc aagccatagc tcattatcag ttttatcatt ctgaaaatat ttttttgctt
      901 caagaacgtg atttagatga attaccggaa tttttgcata cgccatttca accaatccca
      961 tcagaactgt tggaattttt tgatgcagaa aattgggcaa aacgtttttt cactactgac
     1021 tccgctattt atgaacaata tgaatacgat ctttcctctg ttaaaaataa ataaaagtgc
     1081 caaccaatca tagatgaaaa taaacaagcg caattcttat ggcaacatga gaattgtgtt
     1141 tttctttttg aactttcatg ggggtaaaaa aatgagaacc agctgctgat ccagctgatt
     1201 ctcatcagag agccttttat tctgcgccaa ggattccttc ttttgcatgg actaattggg
     1261 tcaaaaaggc acaatatggt gtagcaatat catatttttg tccttttcgc caaacggcac
     1321 cattgatgta gtcgatttca gtcaaacgat ggtttttgat caggtcttgg tacatagatg
     1381 ggtaatgcaa cccgatgttg tcaggatcat aagtagaagc aatatgttgg tacacttctt
     1441 cttgatctaa tgtgattcct tcttttgctg cgatagaagc aaactcagat acaatcgttc
     1501 gaaccatgcc ctctgccgct ttttgcgcac ctaattcagc aatattacaa tccaatatcg
     1561 tgcataatcc atttaatgtt ccgtttacac aagcttttcg ccaaatggaa taacgaacat
     1621 tatgactata gattggattc aattttgctt cttgaaagac ttctacaact ttttttgtaa
     1681 atgcttcgcc ttctggttcc aagttctcta gttcgatttc tccatcacct aataatgtga
     1741 cttttccggg accagctaga ccagctgtcc acatcgtgat cccaaataag atatttttct
     1801 ttggtacata tttttccagt acatcttcat gacctaatcc attcagtaaa cacaaaacat
     1861 aagtattctc agtaataatg gattggatcg aacgaaacat atcatctaat tgattcgctt
     1921 ttgtcaaggc tacgatcaaa tccacgtgtt tgttgctttc gatgatttct tctggtgaat
     1981 agattggcag ttttgcaact acttctttgc cattaaagtc agcaatcaat ccatttgtac
     2041 gaatggcttc aatgtgagca ggccagcgat cgatcaagac gacctcattt ccgctttgat
     2101 gcaacattaa tccgattcgg ctgcccattg ctccggctcc agctattgcg attttcatgt
     2161 tttttctctc ctttattcaa tgagttccct tttagtatat agaaaaacag aagaaaatag
     2221 cattgttttg tttgaaaaaa gtgaaaaaaa gtgtcttact taagaagaaa gtgagggata
     2281 tagatggata ttttggcggc aatcgattca tttaaaggaa gtgcttcttc atttgaatta
     2341 aatcaggcag ttctaaatgg ttgggaagac cgtaaaggaa gaaaaatcaa tgtgccgatt
     2401 gcagatggcg gagaaaatac tgcagaagca atctattacg cgctcggggg aacctggcgt
     2461 acgattgatg ggatggatct cttatttcgt cagcgaaaaa tcagttattt attgacgaac
     2521 tggcaaggaa aaaataccgc agtcattgaa tctgcacaag tattaggatt agatttactt
     2581 gataaaccaa cggatcaaac catccgactt gcttctagtt atgccttggg agagcttatc
     2641 aaagatacac tgcttcaaga agtggaacaa atcattgtca ctttaggagg aagcggatgt
     2701 agcgatggtg gtctaggatt actccaatct ttgggtgctt ctttagaagg gtacacagaa
     2761 ggaaatcctt tgttatctac tagaaaactt catttggatc gaaggaacgt tcttgtttct
     2821 aaagtaaaag tgatcatcgc tgcagacgtg accaacccat atacaggtca acaaggagct
     2881 gcttggcaat ttggaagaca aaaaggagga acggatacga cgttagcttt cttggataaa
     2941 caagctgaaa agattgctca gcagataaaa gatgtttggg aggtggattt agatgacatc
     3001 ccaggaagcg gagcagcagg cggtattggc ggagcccttt ttttgttagg agcgaagatg
     3061 gtctctggtt ttgagttggt cagccaaatc actggattag aaaaacagat cgaacaagcg
     3121 gatgtcatca ttacaggaga agggcggatt gatcagcaaa caatccacgg aaaagttcca
     3181 tatggcgtag cagtgcttgc aaaaaaatat cgaaaaccaa taatcgctat ttgcggaagt
     3241 agagaccgag agctggaagg actctttgat tttctcccca ttgtcttaag cattcaatta
     3301 ggaccaatcg atttagagga agcaatggaa cacaggcaaa cattagaaaa aacagaaatc
     3361 ttaggacaga cactcagcag atttttcaac attctcccga aatagttgac aaaaacaatt
     3421 tctttatgct atcgtaattt caaatatagg acaaagtgag ggatccgtgc attgaaaaat
     3481 taagtcgatt gatgaaagct gcattcagac tgtaccttgt ggagacaagg gagcagtgtg
     3541 cagattttcg ggattggctt ggttctttcg atgtatgggt ctttttgtgt gccccaaccc
     3601 ttgcatcctc tttagccagt tctgaaaata ggactggtct ttttttacga ggtgatggaa
     3661 taatgtctaa aatcgaaata aaaaatctga cattcggcta tgacagccaa ggcacattat
     3721 tatttgaaca agccaatcta aactttgaca cacaatggaa actaggactt atcggacgaa
     3781 acggtcgagg aaagacaact ttactgaata tcctgcaaaa caaactccct tatcaagggc
     3841 aagtgatcca tcagcaagcg tttgtctatt tcccgcagca aacaaaagat aaagagcgtt
     3901 tgacctatta tgtgttaaat gagattatgg attttgagat atgggaaatt gaaagagagc
     3961 tccaactgat gcaaacagat cctgaaatct tatggagaga attcagcaca ctatcaggag
     4021 gagagaagac aaaagtccta ctggcactct tatttgtgga tgacactcat ttcccgttaa
     4081 tcgatgaacc aacgaatcat ttggatatct ctggcagaaa acaagtagcg gcttacttga
     4141 aaaagaaaaa acaaggattc atcgtggtga gtcatgatcg gggatttatc gatgaagtag
     4201 tggaccatgt tttagcaatc gaaaagagcc aactggaact ttatcaaggg aatttctcta
     4261 tctatgaaga acagaaaaaa cttcgtgatg aatttgaaat agctaaaaat gaaaaattga
     4321 aaaaagaagt cagcaggcta aagaaaacag cagctgaaaa agcagaatgg tctcgttccc
     4381 gagaagggga caaaacaaag aaaaaagtcg gattcatcga tacggaatct aggcgagtga
     4441 ataaaggagc agtaggcgct gatgctgcac ggacgatgaa acgatctaaa gcaatcgtga
     4501 atcggatgga aacccagatc agtgagaaag aaaaattatt aaaagatatc gagtatatcg
     4561 atgctttgac gatgaatagc caagtgtctc atcataaacg gctgttaagc gtagaaaatc
     4621 ttcaattagg gtatgaaaat ctgttattcg agccagttca ttttacgatc gaacctcatc
     4681 agcggatggc aatttcaggt cctaacggcg cgggaaagtc atccattatc cattatcttc
     4741 tggggacatt caacggcaag gttgtaggag aaaaatccca gcccaaacat ttgaccatta
     4801 gttacgtacg ccaaaattat gaagacaatc gagggacatt ggcggaattc gcgaagaaaa
     4861 accgagtaga ctatcaagca tttttgaata atctccgaaa gcttgggatg gaaagagatg
     4921 tttttcataa taaaatcgaa cagatgagta tgggacaacg gaaaaaagtg gaattggcta
     4981 aatctttatc ccaaccagct gagctctata tatgggatga accattgaat tatttggatg
     5041 tcttcaatca agaacaatta gaacaactga ttttgaacgt gaaacctgct atgttactag
     5101 tggaacatga tcaaaccttt ctagataaag tatctactga gattatttct cttgagaaaa
     5161 tctaaacctt gtaatctcaa agacaacaag gtatggttaa ggagaacttt gtcaacgaag
     5221 gagagaatca catgctttat gtaacgagac acggagaaac aacttggaac gcacaaggat
     5281 tagtttgcgg gcgagcagat gtgccgctga ctgaaaaagg acagatgcag gcacaggcac
     5341 tagcagaaaa ggtagtagat ttgcctgttc caatcacgaa aatcatccat tcccctcttc
     5401 aacgagcaag agatacagca caagcagtag ttgatcgtct caatttgccg atgaccgctg
     5461 atgagcgatt ggtagaaatg gattttgggg attatgacgg actgccaagc aaagatgaaa
     5521 actttcaaaa agcgcgttta gcgtttgctg tacgtttccc aaatggagag tcggtcttag
     5581 atgtgtacgc acggatcgtt cctttattga aagaatgcat ggaagacgaa gaaaatgtct
     5641 acttgttagt ctgtcataat gccttgataa gagtgatcaa tgcttatttt catccaatgc
     5701 ctaatgaagg gttctttaca ttcatggtag acaatacaga attaatttct tatgagtaat
     5761 tagacttttg tgttatttaa ataaaatgtt tc
//
LOCUS       DAZKLD010000339         4803 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.guided.169, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000339 DAZKLD010000000
VERSION     DAZKLD010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4803)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4803)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4803
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.guided.169"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            92..787
                     /gene="vanR-A"
                     /locus_tag="ACG8KR_003059"
     CDS             92..787
                     /gene="vanR-A"
                     /locus_tag="ACG8KR_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance response regulator
                     transcription factor VanR-A"
                     /protein_id="HFQ0202326.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            765..1919
                     /locus_tag="ACG8KR_003060"
     CDS             765..1919
                     /locus_tag="ACG8KR_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance histidine kinase VanS-A"
                     /protein_id="HFQ0202327.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
     gene            2134..3102
                     /locus_tag="ACG8KR_003061"
     CDS             2134..3102
                     /locus_tag="ACG8KR_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HFQ0202328.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            3095..4126
                     /gene="vanA"
                     /locus_tag="ACG8KR_003062"
     CDS             3095..4126
                     /gene="vanA"
                     /locus_tag="ACG8KR_003062"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HFQ0202329.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALVVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            4132..4740
                     /locus_tag="ACG8KR_003063"
     CDS             4132..4740
                     /locus_tag="ACG8KR_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HFQ0202330.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPKRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
BASE COUNT         1482 a          922 c         1119 g         1280 t
ORIGIN      
        1 catggccttt tatcttttac acaattatat ggactttatc ctataatctg ataaattatt
       61 aagggcaaac ttatgtgaaa gggtgataac tatgagcgat aaaatactta ttgtggatga
      121 tgaacatgaa attgccgatt tggttgaatt atacttaaaa aacgagaatt atacggtttt
      181 caaatactat accgccaaag aagcattgga atgtatagac aagtctgaga ttgaccttgc
      241 catattggac atcatgcttc ccggcacaag cggccttact atctgtcaaa aaataaggga
      301 caagcacacc tatccgatta tcatgctgac cgggaaagat acagaggtag ataaaattac
      361 agggttaaca atcggcgcgg atgattatat aacgaagccc tttcgcccac tggagttaat
      421 tgctcgggta aaggcccagt tgcgccgata caaaaaattc agtggagtaa aggagcagaa
      481 cgaaaatgtt atcgtccact ccggccttgt cattaatgtt aacacccatg agtgttatct
      541 gaacgagaag cagttatccc ttactcccac cgagttttca atactgcgaa tcctctgtga
      601 aaacaagggg aatgtggtta gctccgagct gctatttcat gagatatggg gcgacgaata
      661 tttcagcaag agcaacaaca ccatcaccgt gcatatccgg catttgcgcg aaaaaatgaa
      721 cgacaccatt gataatccga aatatataaa aacggtatgg ggggttggtt ataaaattga
      781 aaaataaaaa aaacgactat tccaaactag aacgaaaact ttacatgtat atcgttgcaa
      841 ttgttgtggt agcaattgta ttcgtgttgt atattcgttc aatgatccga gggaaacttg
      901 gggattggat cttaagtatt ttggaaaaca aatatgactt aaatcacctg gacgcgatga
      961 aattatatca atattccata cggaacaata tagatatctt tatttatgtg gcgattgtca
     1021 ttagtattct tattctatgt cgcgtcatgc tttcaaaatt cgcaaaatac tttgacgaga
     1081 taaataccgg cattgatgta cttattcaga acgaagataa acaaattgag ctttctgcgg
     1141 aaatggatgt tatggaacaa aagctcaaca cattaaaacg gactctggaa aagcgagagc
     1201 aggatgcaaa gctggccgaa caaagaaaaa atgacgttgt tatgtacttg gcgcacgata
     1261 ttaaaacgcc ccttacatcc attatcggtt atttgagcct gcttgacgag gctccagaca
     1321 tgccggtaga tcaaaaggca aagtatgtgc atatcacgtt ggacaaagcg tatcgactcg
     1381 aacagctaat cgacgagttt tttgagatta cacggtataa cctacaaacg ataacgctaa
     1441 caaaaacgca catagaccta tactatatgc tggtgcagat gaccgatgaa ttttatcctc
     1501 agctttccgc acatggaaaa caggcggtta ttcacgcccc cgaggatctg accgtgtccg
     1561 gcgaccctga taaactcgcg agagtcttta acaacatttt gaaaaacgcc gctgcataca
     1621 gtgaggataa cagcatcatt gacattaccg cgggcctctc cggggatgtg gtgtcaatcg
     1681 aattcaagaa cactggaagc atcccaaaag ataagctagc tgccatattt gaaaagttct
     1741 ataggctgga caatgctcgt tcttccgata cgggtggcgc gggacttgga ttggcgattg
     1801 caaaagaaat tattgttcag catggagggc agatttacgc ggaaagcaat gataactata
     1861 cgacgtttag ggtagagctt ccagcgatgc cagacttggt tgataaaagg aggtcctaag
     1921 agatgtatat aattttttag gaaaatctca aggttatctt tactttttct taggaaatta
     1981 acaatttaat attaagaaac ggctcgttct tacacggtag acttaatacc gtaagaacga
     2041 gccgttttcg ttcttcagag aaagatttga caagattacc attggcatcc ccgttttatt
     2101 tggtgccttt cacagaaagg gttggtctta attatgaata acatcggcat tactgtttat
     2161 ggatgtgagc aggatgaggc agatgcattc catgctcttt cgcctcgctt tggcgttatg
     2221 gcaacgataa ttaacgccaa cgtgtcggaa tccaacgcca aatccgcgcc tttcaatcaa
     2281 tgtatcagtg tgggacataa atcagagatt tccgcctcta ttcttcttgc gctgaagaga
     2341 gccggtgtga aatatatttc tacccgaagc atcggctgca atcatataga tacaactgct
     2401 gctaagagaa tgggcatcac tgtcgacaat gtggcgtact cgccggatag cgttgccgat
     2461 tatactatga tgctaattct tatggcagta cgcaacgtaa aatcgattgt gcgctctgtg
     2521 gaaaaacatg atttcaggtt ggacagcgac cgtggcaagg tactcagcga catgacagtt
     2581 ggtgtggtgg gaacgggcca gataggcaaa gcggttattg agcggctgcg aggatttgga
     2641 tgtaaagtgt tggcttatag tcgcagccga agtatagagg taaactatgt accgtttgat
     2701 gagttgctgc aaaatagcga tatcgttacg cttcatgtgc cgctcaatac ggatacgcac
     2761 tatattatca gccacgaaca aatacagaga atgaagcaag gagcatttct tatcaatact
     2821 gggcgcggtc cacttgtaga tacctatgag ttggttaaag cattagaaaa cgggaaactg
     2881 ggcggtgccg cattggatgt attggaagga gaggaagagt ttttctactc tgattgcacc
     2941 caaaaaccaa ttgataatca atttttactt aaacttcaaa gaatgcctaa cgtgataatc
     3001 acaccgcata cggcctatta taccgagcaa gcgttgcgtg ataccgttga aaaaaccatt
     3061 aaaaactgtt tggattttga aaggagacag gagcatgaat agaataaaag ttgcaatact
     3121 gtttgggggt tgctcagagg agcatgacgt atcggtaaaa tctgcaatag agatagccgc
     3181 taacattaat aaagaaaaat acgagccgtt atacattgga attacgaaat ctggtgtatg
     3241 gaaaatgtgc gaaaaacctt gcgcggaatg ggaaaacgac aattgctatt cagctgtact
     3301 ctcgccggat aaaaaaatgc acggattact tgttaaaaag aaccatgaat atgaaatcaa
     3361 ccatgttgat gtagcatttt cagctttgca tggcaagtca ggtgaagatg gatccataca
     3421 aggtctgttt gaattgtccg gtatcccttt tgtaggctgc gatattcaaa gctcagcaat
     3481 ttgtatggac aaatcgttga catacatcgt tgcgaaaaat gctgggatag ctactcccgc
     3541 cttttgggtt attaataaag atgataggcc ggtggcagct acgtttacct atcctgtttt
     3601 tgttaagccg gcgcgttcag gctcatcctt cggtgtgaaa aaagtcaata gcgcggacga
     3661 attggactac gcaattgaat cggcaagaca atatgacagc aaaatcttaa ttgagcaggc
     3721 tgtttcgggc tgtgaggtcg gttgtgcggt attgggaaac agtgccgcgt tagttgttgg
     3781 cgaggtggac caaatcaggc tgcagtacgg aatctttcgt attcatcagg aagtcgagcc
     3841 ggaaaaaggc tctgaaaacg cagttataac cgttcccgca gacctttcag cagaggagcg
     3901 aggacggata caggaaacgg caaaaaaaat atataaagcg ctcggctgta gaggtctagc
     3961 ccgtgtggat atgtttttac aagataacgg ccgcattgta ctgaacgaag tcaatactct
     4021 gcccggtttc acgtcataca gtcgttatcc ccgtatgatg gccgctgcag gtattgcact
     4081 tcccgaactg attgaccgct tgatcgtatt agcgttaaag gggtgataag catggaaata
     4141 ggatttactt ttttagatga aatagtacac ggtgttcgtt gggacgctaa atatgccact
     4201 tgggataatt tcaccggaaa accggttgac ggttatgaag taaatcgcat tgtagggaca
     4261 tacgagttgg ctgaatcgct tttgaaggca aaagaactgg ctgctaccca agggtacgga
     4321 ttgcttctat gggacggtta ccgtcctaag cgtgctgtaa actgttttat gcaatgggct
     4381 gcacagccgg aaaataacct gacaaaggaa agttattatc ccaatattga ccgaactgag
     4441 atgatttcaa aaggatacgt ggcttcaaaa tcaagccata gccgcggcag tgccattgat
     4501 cttacgcttt atcgattaga cacgggtgag cttgtaccaa tggggagccg atttgatttt
     4561 atggatgaac gctctcatca tgcggcaaat ggaatatcat gcaatgaagc gcaaaatcgc
     4621 agacgtttgc gctccatcat ggaaaacagt gggtttgaag catatagcct cgaatggtgg
     4681 cactatgtat taagagacga accatacccc aatagctatt ttgatttccc cgttaaataa
     4741 acttttaacc gttgcacgga caaacggttc tgttgcaaag ttttaaataa agaataaaat
     4801 ccc
//
LOCUS       DAZKLD010000340         3686 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.guided.192, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000340 DAZKLD010000000
VERSION     DAZKLD010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3686)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3686)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3686
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.guided.192"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..230
                     /locus_tag="ACG8KR_003064"
     CDS             <1..230
                     /locus_tag="ACG8KR_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0202331.1"
                     /translation="DFILYLKLCNRTLLVEDASLSGENERINELIEFIRKNKFRTLEY
                     DDKLVRKIIQSVTVYEGHFVIAFKSGIEMEI"
     gene            373..597
                     /locus_tag="ACG8KR_003065"
     CDS             373..597
                     /locus_tag="ACG8KR_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018579902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0202332.1"
                     /translation="MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLD
                     ELKAIADVLGVKFVQAFILPDGEEIKISNE"
     gene            612..1481
                     /locus_tag="ACG8KR_003066"
     CDS             612..1481
                     /locus_tag="ACG8KR_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HFQ0202333.1"
                     /translation="MVGNVIQIVTEKLSSLPFIEGIVLGGSRARGTHTENSDIDIGIY
                     YNSDSFDLTAINQIATELDDKNRNNLVVPPGAWGDWINGGGWLFINGYHVDLILRDIK
                     RVEQIIKDTEQGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAEIY
                     PTALKKSLMNFFIFEAEFSLMFVKANAGVEDKYYIAGHVFRIISCLNQVLFACNNAYC
                     INEKKAIKLLETFEYKPEKYAERVNHIFEVLGLSLFECYDMTEKLYKEVNEIVSEINN
                     FLNEESSDERKQI"
     gene            1462..2196
                     /locus_tag="ACG8KR_003067"
     CDS             1462..2196
                     /locus_tag="ACG8KR_003067"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008425 -
                     2-polyprenyl-6-methoxy-1,4-benzoquinone methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0061542 - 3-demethylubiquinol-n
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0102208 - 2-polyprenyl-6-hydroxyphenol
                     methylase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFQ0202334.1"
                     /translation="MKENKYDDNIFFQKYSQMSRSQKGLAGAGEWETLKKMLPDFKGK
                     RVLDLGCGYGWHCIYAMENGASSVVGVDISHKMLEVAKGKTHFPQIEYECCAIEDVDF
                     PEESFDVILSSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNE
                     KGEILHFPVDNYYYEGKRTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPE
                     NMMDIPGMADEMRRPMMLIVSAKKKM"
     gene            2229..3137
                     /locus_tag="ACG8KR_003068"
     CDS             2229..3137
                     /locus_tag="ACG8KR_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(6)-Ia"
                     /protein_id="HFQ0202335.1"
                     /translation="MRSEKEMMDLVLSLAEQDERIRIVTLEGSRANINIPKDEFQDYD
                     ITYFVSDIEPFISNDDWLNQFGNIIMMQKPEDMELFPPEEKGFSYLMLFDDYNKIDLT
                     LLPLEELDNYLKGDKLIKVLIDKDCRIKRDIVPTDIDYHVRKPSAREYDDCCNEFWNV
                     TPYVIKGLCRKEILFAIDHFNQIVRHELLRMISWKVGIETGFKLSVGKNYKFIERYIS
                     EDLWEKLLSTYRMDSYENIWEALFLCHQLFRAVSGEVAERLHYAYPEYDRNITKYTRD
                     MYKKYTGKTGCLDSTYAADIEERREQ"
     gene            3134..3614
                     /locus_tag="ACG8KR_003069"
                     /pseudo
     CDS             3134..3614
                     /locus_tag="ACG8KR_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
BASE COUNT         1279 a          533 c          801 g         1073 t
ORIGIN      
        1 gggattttat tctttattta aaactttgca acagaaccct tttagtagaa gatgcctccc
       61 tcagtggcga gaacgagcga atcaatgagc tgattgaatt tatccgcaag aacaaattcc
      121 gcaccttaga atatgatgat aagcttgtaa ggaagataat ccagagcgtt acagtctatg
      181 aaggccactt cgtcatagcc ttcaaatctg gcatcgaaat ggaaatatga aaaccagaag
      241 taaatagccc atgactctac agtagagttg tgggttttct tttgcttgct gattataata
      301 atattttaat aactattgtg tttatcaacc aaatggttta taatattctt agcgtaaact
      361 tggaggtgct gtatgactac ggcagaaatg attaaagaac tgtgtgagca aatgaatata
      421 agtgtttccg aacttgctag acgtattggc cagactccac agaatttcaa taaaaaatta
      481 caacgagaaa cagtaacctt ggatgagttg aaggccattg ctgatgtgct aggtgtcaag
      541 tttgtgcagg catttatttt accagatgga gaagaaataa aaatatctaa cgaataaagg
      601 aggatgttta tatggtaggt aatgttattc aaatagtaac agagaaatta tcatctttgc
      661 cttttataga aggcatcgta ttagggggct cacgtgcaag aggcacccat acagaaaatt
      721 ctgatataga tatcggaatc tattacaatt cagactcatt tgacctgaca gcgattaatc
      781 aaattgctac agaattggat gataaaaata gaaacaacct tgttgtgcct cccggagcgt
      841 ggggtgattg gattaatggc ggcggatggt tatttataaa tgggtatcat gttgacttga
      901 ttttacgtga tataaaacgg gtagaacaaa taatcaaaga tacggagcaa ggaattgtta
      961 ctgccaatta tcagaccggg catccccatg gttatataag tgcaatgtat cgtggagaat
     1021 tagcgattag taaaatacta tatgctaaga acgaaagctt atgcgaatta aaaaagcagg
     1081 cagaaattta ccctactgct ctaaagaaaa gcttgatgaa cttttttata tttgaagctg
     1141 aattctcttt aatgtttgta aaagcaaatg cgggggtaga ggataaatat tatattgcag
     1201 gccatgtttt tcgtattatt tcatgcttaa atcaagtact atttgcatgt aataatgctt
     1261 attgcattaa cgaaaagaaa gctataaaac tgcttgaaac ttttgaatat aaacctgaaa
     1321 aatatgccga aagagttaat catatttttg aagtactcgg tctttctctt tttgaatgct
     1381 acgatatgac cgagaaactt tataaagaag tgaatgaaat tgtatcggag ataaataact
     1441 ttttaaacga ggagagttca gatgaaagaa aacaaatatg atgataatat attttttcaa
     1501 aaatacagtc aaatgagtcg ctcgcagaaa ggactggctg gtgcgggaga atgggagact
     1561 ttgaaaaaga tgctacctga ttttaagggt aagcgtgtgc ttgatttagg atgcggctat
     1621 ggatggcact gtatatatgc gatggaaaac ggtgcttcct ctgtagtagg tgttgatatt
     1681 tctcataaaa tgctcgaagt agcaaaagga aaaacccatt ttccacagat tgaatatgaa
     1741 tgctgtgcca tagaagatgt ggatttccca gaggagagct ttgatgtaat actaagttcg
     1801 cttgcgtttc attatgtagc agactatgag aatttaataa aaaagatata taggatgctg
     1861 aaggctggtg gcaatttagt ttttacagtt gaacatcctg tttttactgc tcatggaaca
     1921 caagactggt attataacga aaaaggagaa atactgcatt tcccggtgga caattattat
     1981 tatgagggca aacggacagc tatgtttttg gaagaaaagg ttacaaaata tcatagaaca
     2041 ctgaccacat atctaaatac actgctttca aatagtttta taataaatca gattgtggag
     2101 ccacagccgc cagagaacat gatggatatt ccggggatgg cggatgaaat gcgacgccca
     2161 atgatgctga ttgtatcggc aaaaaagaag atgtaataat atagaaaaaa taaacgagga
     2221 gtatgtaaat gagatcagaa aaagaaatga tggatttagt actttcttta gcagaacagg
     2281 atgaacgtat tcgaattgtg acccttgagg ggtcacgcgc aaatattaat atacctaaag
     2341 atgaatttca ggattatgat attacatatt ttgtaagtga tatagaaccg tttatatcta
     2401 atgatgactg gcttaatcaa tttgggaata taataatgat gcaaaagccg gaggatatgg
     2461 aattattccc acctgaagaa aagggatttt cctatcttat gctatttgat gattacaata
     2521 aaattgatct taccttattg cccttggaag agttagataa ttacctaaag ggcgataaat
     2581 taataaaggt tctaattgat aaagattgta gaattaaaag ggacatagtt ccgactgata
     2641 tagattatca tgtaagaaag ccaagcgcaa gggagtatga tgattgctgc aatgaatttt
     2701 ggaatgtaac accttatgtt attaaaggat tgtgccgtaa ggaaatttta tttgctattg
     2761 atcattttaa tcagattgtt cgccatgagc tgctgagaat gatatcatgg aaggtcggca
     2821 tcgaaacagg ctttaaatta agtgtaggca agaactataa gtttattgaa aggtatatat
     2881 ccgaggattt gtgggagaaa cttttgtcca cctaccggat ggattcctat gaaaacatat
     2941 gggaagcatt atttctatgc catcaattgt tcagggcggt atccggtgag gtggcggaaa
     3001 ggcttcatta tgcctatccg gagtatgata ggaatataac aaaatatacc agggacatgt
     3061 ataaaaaata cactggtaaa accggctgcc tggatagcac atatgccgct gatatagaag
     3121 agaggcggga acagtgatta cagaaatgaa agcagggcac ctgaaagata tcgataaacc
     3181 cagcgaacca tttgaggtga taggtaagat tataccgagg tatgaaaacg agaattggac
     3241 ctttacagaa ttactctatg aagcgccata tttaaaaagc taccaagacg aagaggatga
     3301 agaggatgag gaggcagatt gccttgaata tattgacaat actgataaga taatatatct
     3361 tttatataga agatatcgcc gtatgtaagg atttcagggg gcaaggcata ggcagcgcgc
     3421 ttatcaatat atctatagaa tgggcaaagc ataaaaactt gcatggacta atgcttgaaa
     3481 cccaggacaa taaccttata gcttgtaaat tctatcataa ttgtggtttc aaaatcggct
     3541 ccgtcgatac tatgttatac gccaactttg aaaacaactt tgaaaaagct gttttctggt
     3601 atttaaggtt ttagaatgca aggaacagtg aattggagtt cgtcttgtgg ttctgttgca
     3661 aagttttaaa taaagaataa aatccc
//
LOCUS       DAZKLD010000341         3190 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.guided.206, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000341 DAZKLD010000000
VERSION     DAZKLD010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3190)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3190)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3190
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.guided.206"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            260..1171
                     /locus_tag="ACG8KR_003070"
     CDS             260..1171
                     /locus_tag="ACG8KR_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase VanY-A"
                     /protein_id="HFQ0202336.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYEPWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHTNSRR"
     gene            1324..1809
                     /gene="vanZ-A"
                     /locus_tag="ACG8KR_003071"
     CDS             1324..1809
                     /gene="vanZ-A"
                     /locus_tag="ACG8KR_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance protein VanZ-A"
                     /protein_id="HFQ0202337.1"
                     /translation="MGKILSRGLLALYLVTLIWLVLFKLQYNILSVFNYHQRSLNLTP
                     FTATGNFREMIDNVIIFIPFGLLLNVNFKEIGFLPKFAFVLVLSLTFEIIQFIFAIGA
                     TDITDVITNTVGGFLGLKLYGLSNKHMNQKKLDRVIIFVGILLLVLLLVYRTHLRINY
                     V"
     gene            2328..2612
                     /locus_tag="ACG8KR_003072"
     CDS             2328..2612
                     /locus_tag="ACG8KR_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFQ0202338.1"
                     /translation="MELAFRESLKKMRGTKSKEKFSQELEMSRSNYSRIESGKSDPTI
                     KTLEQIAKLTNSTLVVDLIPNEPTEPEPETESEQVTLDLEMEEEKSNDFV"
     gene            2978..>3190
                     /locus_tag="ACG8KR_003073"
     CDS             2978..>3190
                     /locus_tag="ACG8KR_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0202339.1"
                     /translation="MNHDECKTYIKNSLLEIRKLANIYTLETFKKELEKRNIYLETKS
                     DKYFSSEGEDYIYKLIENNKIIYSISG"
BASE COUNT         1188 a          448 c          587 g          967 t
ORIGIN      
        1 ccttatttga tagtagattt aaaactttgc aacagaaccc ccattgtcaa caggcagttc
       61 agcctcgtta aattcagcat gggtatcact tatgaaaatt catctacatt ggtgataata
      121 gtaaatccag tagggcgaaa taattgactg taatttacgg ggcaaaacgg cacaatctca
      181 aacgagattg tgccgtttaa ggggaagatt ctagaaatat ttcatacttc caactatata
      241 gttaaggagg agactgaaaa tgaagaagtt gtttttttta ttgttattgt tattcttaat
      301 atacttaggt tatgactacg ttaatgaagc actgttttct caggaaaaag tcgaatttca
      361 aaattatgat caaaatccca aagaacattt agaaaatagt gggacttctg aaaataccca
      421 agagaaaaca attacagaag aacaggttta tcaaggaaat ctgctattaa tcaatagtaa
      481 atatcctgtt cgccaagaaa gtgtgaagtc agatatcgtg aatttatcta aacatgacga
      541 attaataaat ggatacgggt tgcttgatag taatatttat atgtcaaaag aaatagcaca
      601 aaaattttca gagatggtca atgatgctgt aaagggtggc gttagtcatt ttattattaa
      661 tagtggctat cgagactttg atgagcaaag tgtgctttac caagaaatgg gggctgagta
      721 tgccttacca gcaggttata gtgagcataa ttcaggttta tcactagatg taggatcaag
      781 cttgacgaaa atggaacgag cccctgaagg aaagtggata gaagaaaatg cttggaaata
      841 cgggttcatt ttacgttatc cagaggacaa aacagagtta acaggaattc aatatgaacc
      901 atggcatatt cgctatgttg gtttaccaca tagtgcgatt atgaaagaaa agaatttcgt
      961 tctcgaggaa tatatggatt acctaaaaga agaaaaaacc atttctgtta gtgtaaatgg
     1021 ggaaaaatat gagatctttt attatcctgt tactaaaaat accaccattc atgtgccgac
     1081 taatcttcgt tatgagatat caggaaacaa tatagacggt gtaattgtga cagtgtttcc
     1141 cggatcaaca catactaatt caaggaggta aggatggcgg aatgaaacca acgaaattaa
     1201 tgaacagcat tattgtacta gcacttttgg ggtaacgtta gctttttaat ttaaaaccca
     1261 cgttaactag gacattgcta tactaatgat acaacttaaa caaaagaatt agaggaaatt
     1321 atattgggaa aaatattatc tagaggattg ctagctttat atttagtgac actaatctgg
     1381 ttagtgttat tcaaattaca atacaatatt ttatcagtat ttaattatca tcaaagaagt
     1441 cttaacttga ctccatttac tgctactggg aatttcagag agatgataga taatgttata
     1501 atctttattc catttggctt gcttttgaat gtcaatttta aagaaatcgg atttttacct
     1561 aagtttgctt ttgtactggt tttaagtctt acttttgaaa taattcaatt tatcttcgct
     1621 attggagcga cagacataac agatgtaatt acaaatactg ttggaggctt tcttggactg
     1681 aaattatatg gtttaagcaa taagcatatg aatcaaaaaa aattagacag agttattatt
     1741 tttgtaggta tacttttgct cgtattattg ctcgtttacc gtacccattt aagaataaat
     1801 tacgtgtaag atgtctaaat caagcaatct gatctttcat acacataaag atattgaatg
     1861 aattggatta gatggaaaac gggatgtggg gaaactcgcc cgtaggtgtg aagtgagggg
     1921 aaaaccggtg ataaagtaaa aagcttacct aacactatag taacaaagaa agcccaatta
     1981 tcaattttag tgctgaggaa ttggtctctt taataaattt ccttaacgtt gtaaatccgc
     2041 attttcctga cggtaccccc actagcagcc atgctttttt tgaatctttg taggacgttt
     2101 taaagagaat tacccacttt aaaattaaac atgcgcttag aatcgcttta ggaaacacga
     2161 tccagtccaa taatcgtcga taaaaacttt tgaaaaaggt tggtgaaatt acctactttt
     2221 ggaataatca caaatcacaa gtgattaatc acttgtttat taagatatta aaagctataa
     2281 tttaaataaa gcgtgaattt tattacacaa aaagaggggg gagaaacttg gaactagcat
     2341 ttagagaaag cttaaaaaag atgagaggta ccaaatcaaa agaaaaattc tcccaagaat
     2401 tagaaatgag tagatcaaat tattcacgaa tagaatcagg aaaatcagat ccaaccataa
     2461 aaacactaga acaaattgca aagttaacta actcaacgct agtagtggat ttaatcccaa
     2521 atgagccaac agaaccagaa ccagaaacag aatcagaaca agtaacattg gatttagaaa
     2581 tggaagaaga aaaaagcaat gacttcgtgt gaataatgca cgaaatcgtt gcttattttt
     2641 tttaaaagcg gtatactaga tataacgaaa caacgaactg aatagaaacg aaaaaagagc
     2701 catgacacat ttataaaatg tttgacgaca ttttataaat gcatagcccg ataagattgc
     2761 caaaccaacg cttatcagtt agtcagatga actcttccct cgtaagaagt tatttaatta
     2821 actttgtttg aagacggtat ataaccgtac tatcattata tagggaaatc agagagtttt
     2881 caagtatcta agctactgaa tttaagaatt gttaagcaat caatcggaaa tcgtttgatt
     2941 gctttttttg tattcattta tagaaggtgg agtttgtatg aatcatgatg aatgtaaaac
     3001 ttatataaaa aatagtttat tggagataag aaaattagca aatatctata cactagaaac
     3061 gtttaagaaa gagttagaaa agagaaatat ctacttagaa acaaaatcag ataagtattt
     3121 ttcttcggag ggggaagatt atatatataa gttaatagaa aataacaaaa taatttattc
     3181 gattagtgga
//
LOCUS       DAZKLD010000342         1276 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.guided.271, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000342 DAZKLD010000000
VERSION     DAZKLD010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1276)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1276)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1276
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.guided.271"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..545
                     /gene="catA"
                     /locus_tag="ACG8KR_003074"
     CDS             <1..545
                     /gene="catA"
                     /locus_tag="ACG8KR_003074"
                     /EC_number="2.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type A chloramphenicol O-acetyltransferase"
                     /protein_id="HFQ0202340.1"
                     /translation="TLFKDMIKKKGYEIYPSLIYAIMEVVNKNKVFRTGINSENKLGY
                     WDKLNPLYTVFNKQTEKFTNIWTESDKNFISFYNNYKNDLLEYKDKEEMFPKKPIPEN
                     TIPISMIPWIDFSSFNLNIGNNSSFLLPIITIGKFYSENNKIYIPVALQLHHSVCDGY
                     HASLFMNEFQDIIHRVDDWI"
BASE COUNT          449 a          147 c          208 g          472 t
ORIGIN      
        1 ttactttgtt taaagatatg ataaaaaaga aaggatatga aatttatccc tctttaattt
       61 atgcaattat ggaagttgta aataaaaata aagtgtttag aacaggaatt aatagtgaga
      121 ataaattagg ttattgggat aagttaaatc ctttgtatac agtttttaat aagcaaactg
      181 aaaaatttac taacatttgg actgaatctg ataaaaactt catttctttt tataataatt
      241 ataaaaatga cttgcttgaa tataaagata aagaagaaat gtttcctaaa aaaccgatac
      301 ctgaaaacac cataccgatt tcaatgattc cttggattga ttttagttca tttaatttaa
      361 atattggtaa caatagcagc tttttattgc ctattattac gataggtaaa ttttatagtg
      421 agaataataa aatttatata ccagttgctc tgcaacttca tcattctgta tgtgatggtt
      481 accatgcttc actatttatg aatgaatttc aagatataat tcatagggta gatgattgga
      541 tttagttttt agattttgaa agtgaattta attttataca cgtaagtgat cataaaattt
      601 atgaacgtat aacaaccaca ttttttggtt gcttgtggtt ttgattttga atttggtttt
      661 gaacttatgg actgatttat tcagtccatt ttttgtgctt gcacaaaaac tagcctcgca
      721 gagcacacgc attaatgact tatgaaacgt agtaaataag tctagtgtgt tatactttac
      781 ttggaagatg caccgaataa aaaatattga agaacaacta gcaaaagatt ttaaagagtt
      841 attttatttt aagtctttat aacatgagtg aagcgaattt ttaaatttcg atagaaattt
      901 ttacatcaaa aagccccctg tcaaaattga cgaagggggt tttttggcgc acgcttttcg
      961 ttagaaatat acaagattga aaatcgtgta taagtgcgcc ctttgttttg aacttagcac
     1021 gttacatcaa ttttttaaaa tgatgtataa gtgcgccctt ttaaattttg agtgattata
     1081 ttttttgagt tagaaaaagg gattgggaaa atttcccaaa ataatttaaa aaataagcaa
     1141 aaattttcga tagagaatgt gctatttttt gtcaaaggtg tataccttga ctgtgcttgc
     1201 tgttacatta agtttatttt taagttatta aaaaagaaat agcttttaaa gtttggctcg
     1261 ctgtcgcttt ataaag
//
LOCUS       DAZKLD010000343         1037 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.guided.284, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000343 DAZKLD010000000
VERSION     DAZKLD010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1037)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1037)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1037
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.guided.284"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            90..827
                     /gene="erm(B)"
                     /locus_tag="ACG8KR_003075"
     CDS             90..827
                     /gene="erm(B)"
                     /locus_tag="ACG8KR_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011100845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HFQ0202341.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESRASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTITYEQVLSIFNSYLLFNGRK"
BASE COUNT          380 a          180 c          171 g          306 t
ORIGIN      
        1 atgacttttt aaacagataa ctaaaattac aaacaaatcg tttaacttct gtatttgttt
       61 atagatgtaa tcacttcagg agagattaca tgaacaaaaa tataaaatat tctcaaaact
      121 ttttaacgag tgaaaaagta ctcaaccaaa taataaaaca attgaattta aaagaaaccg
      181 ataccgttta cgaaattgga acaggtaaag ggcatttaac gacgaaactg gctaaaataa
      241 gtaaacaggt aacgtctatt gaattagaca gtcatctatt caacttatcg tcagaaaaat
      301 taaaactgaa tactcgtgtc actttaattc accaagatat tctacagttt caattcccta
      361 acaaacagag gtataaaatt gttgggaata ttccttacca tttaagcaca caaattatta
      421 aaaaagtggt ttttgaaagc cgtgcgtctg acatctatct gattgttgaa gaaggattct
      481 acaagcgtac cttggatatt caccgaacac tagggttgct cttgcacact caagtctcga
      541 ttcagcaatt gcttaagctg ccagcggaat gctttcatcc taaaccaaaa gtaaacagtg
      601 tcttaataaa acttacccgc cataccacag atgttccaga taaatattgg aagctatata
      661 cgtactttgt ttcaaaatgg gtcaatcgag aatatcgtca actgtttact aaaaatcagt
      721 ttcatcaagc aatgaaacac gccaaagtaa acaatttaag taccattact tatgagcaag
      781 tattgtctat ttttaatagt tatctattat ttaacgggag gaaataattc tatgagtcgc
      841 ttttttaaat ttggaaagtt acacgttact aaagggaatg gagataaatt attagatata
      901 ctactgacag cttccaagaa gctaaagagg tccctagcgc ctacggggaa tttgtatcga
      961 taaggggtac aaattcccac taagcgctcg ggaccccttg taggaaaatg tcctaagtgt
     1021 ggcaacaata ttgtatt
//
LOCUS       DAZKLD010000344          919 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.guided.292, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000344 DAZKLD010000000
VERSION     DAZKLD010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 919)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 919)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..919
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.guided.292"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            77..426
                     /locus_tag="ACG8KR_003076"
                     /pseudo
     CDS             77..426
                     /locus_tag="ACG8KR_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741963.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            complement(698..>919)
                     /locus_tag="ACG8KR_003077"
     CDS             complement(698..>919)
                     /locus_tag="ACG8KR_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0202342.1"
                     /translation="FSLNGVNCLIASHSLNFLAEVDEGMKLVVTGYYNNRKQFVVRSY
                     HVLGKPKIVVEYEKSLYPRKKVRQDERIN"
BASE COUNT          288 a          180 c          138 g          313 t
ORIGIN      
        1 atatttccta atagagtata acatagcttt aagcattact taaagataac ttaaaagtta
       61 tcttttaaat acttaattgt taaatacctt atttgatgca aaagaaatct acgatagtca
      121 acaaaaaaat tttcattata tccagaaaaa tttttcttag ttaaggacct atggattgca
      181 gtaatgcaga attcgtcgaa taaactgcca aagacataca atcatattgc ttttaaaatt
      241 gacgaacaag atatagattc gtttgtctca aaaatccaaa tgttaggttt aactgtcgaa
      301 ccaggacgtt ctcgtgtgaa aggcgaggct tcttcaattt acttttacga ctatgataat
      361 cacctcttcg agttacatac cggtactctc caggaacgtt taaaatccta caattccact
      421 acctgagact agtaatgact agtctctttt tatggcctct gatcccccca ttgagcgaat
      481 actaataggg cttcgaattg atctgttcaa tctttaagca atatccctca aatgagtttg
      541 gaaagtttag tgattcatca caaatactta aatacatgta gttatatata gattgaggtt
      601 cttgtgagcg tatgctcgtg tgggttcaaa tcccatcgtc tgcatccatc tttccaaaac
      661 aagcggctag gacatttttg tctcagccgc ttgttttcta gtttattctt tcatcttgtc
      721 ggaccttttt tcttgggtat agacttttct catattctac cacaattttg ggtttcccta
      781 acacatgata ggatctcaca acaaattgct ttctattatt ataataacct gtaacgacta
      841 acttcatccc ttcgtctact tcagctagga agttcaaact atgggaagca atcaaacaat
      901 tgaccccatt taaactgaa
//
LOCUS       DAZKLD010000345          704 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.guided.308, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000345 DAZKLD010000000
VERSION     DAZKLD010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 704)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 704)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..704
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.guided.308"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            91..639
                     /gene="aac(6')"
                     /locus_tag="ACG8KR_003078"
     CDS             91..639
                     /gene="aac(6')"
                     /locus_tag="ACG8KR_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HFQ0202343.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT          253 a          110 c          160 g          181 t
ORIGIN      
        1 atactgattg gtagctaaag atttttgagc gagaacattt cgttttttca aaaggactgt
       61 tagaataagc aagaacaagg gggaagacgt atgataatca gtgaatttga ccgtaataat
      121 ccagtattga aagatcagct ttctgattta ctgagactga cttggccgga agaatatgga
      181 gacagctcgg cagaagaagt agaagaaatg atgaatccag aacgaatcgc ggtagcagcg
      241 gtagaccaag atgagttagt aggatttatt ggtgcaatcc ctcaatacgg tatcacaggt
      301 tgggaattgc atccattagt tgtagaaagc tcccgacgaa agaaccaaat aggtactcga
      361 ttagtcaatt acttagaaaa agaagtagct tccagaggag gaatcacgat ttatttaggt
      421 acggatgatt tagaccatgg aacaacgtta agtcaaacgg acctgtatga gcatacattt
      481 gataaagtgg cttccatcca gaaccttcgt gaacatccgt atgaattcta tgaaaaatta
      541 ggttataaaa tcgtaggtgt cttaccaaat gcaaatggct gggacaaacc ggatatttgg
      601 atggcaaaaa cgattattcc tcgaccaaat tctcaataat aaaaagaggt tgtgacagaa
      661 gtaatcagct tcaagaaata agaaggaatt tacgaaaatt actt
//
LOCUS       DAZKLD010000346          439 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3802
            SAMEA115706932-rid22570133.guided.334, whole genome shotgun
            sequence.
ACCESSION   DAZKLD010000346 DAZKLD010000000
VERSION     DAZKLD010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706932
            Sequence Read Archive: ERR13196666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 439)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 439)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287832.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:42:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 16 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..439
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706932-rid22570133.guided.334"
                     /isolate="VRE3802"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>439
                     /locus_tag="ACG8KR_003079"
     CDS             <1..>439
                     /locus_tag="ACG8KR_003079"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0202344.1"
                     /translation="DFILYLKLCNRTESLKKHYTEPWEDEVFRVIIEYNNVPIGYGQI
                     YKMYDELYTDYHYPKTDEIVYGMDQFIGEPNYWSKGIGTRYIKLIFEFLKKERNANAV
                     ILDPHKNNPRAIRAYQKSGFRIIEDLPEHELHEGKKEDCYLMEY"
BASE COUNT          178 a           44 c           77 g          140 t
ORIGIN      
        1 ggattttatt ctttatttaa aactttgcaa cagaaccgaa tcattaaaaa aacattatac
       61 agagccttgg gaagatgaag tttttagagt aattattgaa tataacaatg ttcctattgg
      121 atatggacaa atatataaaa tgtatgatga gttatatact gattatcatt atccaaaaac
      181 tgatgagata gtctatggta tggatcaatt tataggagag ccaaattatt ggagtaaagg
      241 aattggtaca agatatatta aattgatttt tgaatttttg aaaaaagaaa gaaatgctaa
      301 tgcagttatt ttagaccctc ataaaaataa tccaagagca ataagggcat accaaaaatc
      361 tggttttaga attattgaag atttgccaga acatgaatta cacgagggca aaaaagaaga
      421 ttgttattta atggaatat
//
